LOCUS       DAMZET010000001       149008 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.001, whole genome shotgun sequence.
ACCESSION   DAMZET010000001 DAMZET010000000
VERSION     DAMZET010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 149008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 149008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..149008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.001"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..726)
                     /locus_tag="POB34_000001"
     CDS             complement(<1..726)
                     /locus_tag="POB34_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HDI5917029.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSSRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRK"
     gene            complement(1098..1988)
                     /gene="malG"
                     /locus_tag="POB34_000002"
     CDS             complement(1098..1988)
                     /gene="malG"
                     /locus_tag="POB34_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HDI5917030.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(2003..3547)
                     /gene="malF"
                     /locus_tag="POB34_000003"
     CDS             complement(2003..3547)
                     /gene="malF"
                     /locus_tag="POB34_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HDI5917031.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(3701..4891)
                     /gene="malE"
                     /locus_tag="POB34_000004"
     CDS             complement(3701..4891)
                     /gene="malE"
                     /locus_tag="POB34_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HDI5917032.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            5256..6371
                     /gene="malK"
                     /locus_tag="POB34_000005"
     CDS             5256..6371
                     /gene="malK"
                     /locus_tag="POB34_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HDI5917033.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            6443..7783
                     /gene="lamB"
                     /locus_tag="POB34_000006"
     CDS             6443..7783
                     /gene="lamB"
                     /locus_tag="POB34_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HDI5917034.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            8026..8946
                     /gene="malM"
                     /locus_tag="POB34_000007"
     CDS             8026..8946
                     /gene="malM"
                     /locus_tag="POB34_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HDI5917035.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            9175..10755
                     /locus_tag="POB34_000008"
                     /pseudo
     CDS             9175..10755
                     /locus_tag="POB34_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            10978..11475
                     /gene="ubiC"
                     /locus_tag="POB34_000009"
     CDS             10978..11475
                     /gene="ubiC"
                     /locus_tag="POB34_000009"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HDI5917036.1"
                     /translation="MSHPALTQLRALRYFKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            11488..12360
                     /gene="ubiA"
                     /locus_tag="POB34_000010"
     CDS             11488..12360
                     /gene="ubiA"
                     /locus_tag="POB34_000010"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HDI5917037.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(12515..14938)
                     /gene="plsB"
                     /locus_tag="POB34_000011"
     CDS             complement(12515..14938)
                     /gene="plsB"
                     /locus_tag="POB34_000011"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HDI5917038.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLREEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            15109..15477
                     /gene="dgkA"
                     /locus_tag="POB34_000012"
     CDS             15109..15477
                     /gene="dgkA"
                     /locus_tag="POB34_000012"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDI5917039.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            15587..16195
                     /gene="lexA"
                     /locus_tag="POB34_000013"
     CDS             15587..16195
                     /gene="lexA"
                     /locus_tag="POB34_000013"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HDI5917040.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            16268..17593
                     /gene="dinF"
                     /locus_tag="POB34_000014"
     CDS             16268..17593
                     /gene="dinF"
                     /locus_tag="POB34_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HDI5917041.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            17709..17918
                     /locus_tag="POB34_000015"
     CDS             17709..17918
                     /locus_tag="POB34_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDI5917042.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(17960..18475)
                     /gene="zur"
                     /locus_tag="POB34_000016"
     CDS             complement(17960..18475)
                     /gene="zur"
                     /locus_tag="POB34_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HDI5917043.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            18793..19809
                     /locus_tag="POB34_000017"
     CDS             18793..19809
                     /locus_tag="POB34_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HDI5917044.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNKDANKDKLDNIDATKDKLDNR
                     GTIKGKLNNIYGKSIDYSALRHRDIIIAKIDLFIQRITHNLWHARKKNVFLIEQITNL
                     KVWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNKDATPIERCTFDVE
                     TKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHDDNSLKLFASLLIS
                     LSYSSAYTDLSETVYINENNESYLKAQFEKLSQRDMKKYLGEMKRLADGGEMNFDGYL
                     DKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKILTDTSKLIYDLFGV
                     KSEK"
     gene            20172..21209
                     /gene="dusA"
                     /locus_tag="POB34_000018"
     CDS             20172..21209
                     /gene="dusA"
                     /locus_tag="POB34_000018"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HDI5917045.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            21343..21585
                     /gene="pspG"
                     /locus_tag="POB34_000019"
     CDS             21343..21585
                     /gene="pspG"
                     /locus_tag="POB34_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HDI5917046.1"
                     /translation="MLELLFVFGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(21751..22734)
                     /locus_tag="POB34_000020"
     CDS             complement(21751..22734)
                     /locus_tag="POB34_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HDI5917047.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            22817..24232
                     /gene="dnaB"
                     /locus_tag="POB34_000021"
     CDS             22817..24232
                     /gene="dnaB"
                     /locus_tag="POB34_000021"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDI5917048.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            24285..25364
                     /gene="alr"
                     /locus_tag="POB34_000022"
     CDS             24285..25364
                     /gene="alr"
                     /locus_tag="POB34_000022"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDI5917049.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            25617..26810
                     /gene="tyrB"
                     /locus_tag="POB34_000023"
     CDS             25617..26810
                     /gene="tyrB"
                     /locus_tag="POB34_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HDI5917050.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVMCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(27032..27535)
                     /locus_tag="POB34_000024"
     CDS             complement(27032..27535)
                     /locus_tag="POB34_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917051.1"
                     /translation="MNISYVNSNKTTSLPVELDALNNKDISYAKDFSYAKDFFLYIET
                     QLKIAKDFCRPGEEVSSSIASKVFHAFIDLVNKIRGKKDFMYICTLCCFAEEVKGDYS
                     HYRTFLFDIGNQYKVKLTQSGKKELSLTLEFNDTIIESQKVTGNKAKHILEDIEKFYR
                     NKPDTYY"
     gene            27937..28650
                     /gene="aphA"
                     /locus_tag="POB34_000025"
     CDS             27937..28650
                     /gene="aphA"
                     /locus_tag="POB34_000025"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HDI5917052.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPVT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            28761..29177
                     /locus_tag="POB34_000026"
     CDS             28761..29177
                     /locus_tag="POB34_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HDI5917053.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            29181..29537
                     /locus_tag="POB34_000027"
     CDS             29181..29537
                     /locus_tag="POB34_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDI5917054.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(29572..32394)
                     /gene="uvrA"
                     /locus_tag="POB34_000028"
     CDS             complement(29572..32394)
                     /gene="uvrA"
                     /locus_tag="POB34_000028"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDI5917055.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            32648..33184
                     /gene="ssb1"
                     /locus_tag="POB34_000029"
     CDS             32648..33184
                     /gene="ssb1"
                     /locus_tag="POB34_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HDI5917056.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            complement(33283..33564)
                     /locus_tag="POB34_000030"
     CDS             complement(33283..33564)
                     /locus_tag="POB34_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HDI5917057.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            33994..35580
                     /gene="pdeC"
                     /locus_tag="POB34_000031"
     CDS             33994..35580
                     /gene="pdeC"
                     /locus_tag="POB34_000031"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HDI5917058.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFLDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(35583..35906)
                     /gene="soxS"
                     /locus_tag="POB34_000032"
     CDS             complement(35583..35906)
                     /gene="soxS"
                     /locus_tag="POB34_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HDI5917059.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            35992..36456
                     /gene="soxR"
                     /locus_tag="POB34_000033"
     CDS             35992..36456
                     /gene="soxR"
                     /locus_tag="POB34_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HDI5917060.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            37002..38351
                     /gene="ghxP"
                     /locus_tag="POB34_000034"
                     /gene_synonym="yjcD"
     CDS             37002..38351
                     /gene="ghxP"
                     /locus_tag="POB34_000034"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HDI5917061.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            38502..40151
                     /locus_tag="POB34_000035"
     CDS             38502..40151
                     /locus_tag="POB34_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HDI5917062.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(40305..41597)
                     /locus_tag="POB34_000036"
     CDS             complement(40305..41597)
                     /locus_tag="POB34_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDI5917063.1"
                     /translation="MRYNGLNNMFFSLCQINDNHSFTSSSHTKKTKSYNYSKHHKNTL
                     IDNKALSLFKMDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKHFIDIALYANNK
                     CDDRITRRVYLSKEKEVSIKVVYFINNVAVHNNTIEIPQTVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYIAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTDSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     RQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(41775..43424)
                     /gene="actP"
                     /locus_tag="POB34_000037"
     CDS             complement(41775..43424)
                     /gene="actP"
                     /locus_tag="POB34_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HDI5917064.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(43421..43735)
                     /locus_tag="POB34_000038"
     CDS             complement(43421..43735)
                     /locus_tag="POB34_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HDI5917065.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(43936..45894)
                     /gene="acs"
                     /locus_tag="POB34_000039"
     CDS             complement(43936..45894)
                     /gene="acs"
                     /locus_tag="POB34_000039"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HDI5917066.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            46286..47722
                     /gene="nrfA"
                     /locus_tag="POB34_000040"
     CDS             46286..47722
                     /gene="nrfA"
                     /locus_tag="POB34_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HDI5917067.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            47767..48333
                     /gene="nrfB"
                     /locus_tag="POB34_000041"
     CDS             47767..48333
                     /gene="nrfB"
                     /locus_tag="POB34_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HDI5917068.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            48330..49001
                     /gene="nrfC"
                     /locus_tag="POB34_000042"
     CDS             48330..49001
                     /gene="nrfC"
                     /locus_tag="POB34_000042"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HDI5917069.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            48998..49954
                     /gene="nrfD"
                     /locus_tag="POB34_000043"
     CDS             48998..49954
                     /gene="nrfD"
                     /locus_tag="POB34_000043"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HDI5917070.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPTVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            49974..51692
                     /locus_tag="POB34_000044"
     CDS             49974..51692
                     /locus_tag="POB34_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HDI5917071.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGAVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            51685..52068
                     /gene="nrfF"
                     /locus_tag="POB34_000045"
     CDS             51685..52068
                     /gene="nrfF"
                     /locus_tag="POB34_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HDI5917072.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            52065..52661
                     /gene="nrfG"
                     /locus_tag="POB34_000046"
     CDS             52065..52661
                     /gene="nrfG"
                     /locus_tag="POB34_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HDI5917073.1"
                     /translation="MKQPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASKQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            53003..54316
                     /gene="gltP"
                     /locus_tag="POB34_000047"
     CDS             53003..54316
                     /gene="gltP"
                     /locus_tag="POB34_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HDI5917074.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(54394..55083)
                     /locus_tag="POB34_000048"
     CDS             complement(54394..55083)
                     /locus_tag="POB34_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDI5917075.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(55177..57324)
                     /gene="fdhF"
                     /locus_tag="POB34_000049"
     CDS             complement(55177..57324)
                     /gene="fdhF"
                     /locus_tag="POB34_000049"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(56905..56907),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDI5917076.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(57522..58988)
                     /gene="mdtP"
                     /locus_tag="POB34_000050"
     CDS             complement(57522..58988)
                     /gene="mdtP"
                     /locus_tag="POB34_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HDI5917077.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(58985..61036)
                     /gene="mdtO"
                     /locus_tag="POB34_000051"
     CDS             complement(58985..61036)
                     /gene="mdtO"
                     /locus_tag="POB34_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HDI5917078.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRAMGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(61036..62067)
                     /gene="mdtN"
                     /locus_tag="POB34_000052"
     CDS             complement(61036..62067)
                     /gene="mdtN"
                     /locus_tag="POB34_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HDI5917079.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMIRIGA
                     SAVANLEPQ"
     gene            complement(62086..62361)
                     /locus_tag="POB34_000053"
     CDS             complement(62086..62361)
                     /locus_tag="POB34_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917080.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(62570..64555)
                     /gene="yjcS"
                     /locus_tag="POB34_000054"
     CDS             complement(62570..64555)
                     /gene="yjcS"
                     /locus_tag="POB34_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HDI5917081.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            complement(64828..65757)
                     /gene="alsK"
                     /locus_tag="POB34_000055"
     CDS             complement(64828..65757)
                     /gene="alsK"
                     /locus_tag="POB34_000055"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HDI5917082.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPG
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNPGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPHQVV
                     RFIAASSSDFNGAQGAAILAHQRFLPQSCAKVP"
     gene            complement(65741..66436)
                     /gene="alsE"
                     /locus_tag="POB34_000056"
     CDS             complement(65741..66436)
                     /gene="alsE"
                     /locus_tag="POB34_000056"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HDI5917083.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLATKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHDMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            complement(66447..67427)
                     /gene="alsC"
                     /locus_tag="POB34_000057"
     CDS             complement(66447..67427)
                     /gene="alsC"
                     /locus_tag="POB34_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HDI5917084.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSP
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGV
                     DPFLAAMIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            complement(67406..68938)
                     /gene="alsA"
                     /locus_tag="POB34_000058"
     CDS             complement(67406..68938)
                     /gene="alsA"
                     /locus_tag="POB34_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HDI5917085.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNISYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHETVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNMAISRSLKDGGYKGAMGLFHEVDEQRTAENQ
                     RELLALKCHSVNQNITELSGGNQQKVLISKWLCCCPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     QE"
     gene            complement(69065..70000)
                     /gene="alsB"
                     /locus_tag="POB34_000059"
     CDS             complement(69065..70000)
                     /gene="alsB"
                     /locus_tag="POB34_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HDI5917086.1"
                     /translation="MNKYLKYFSSTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEAFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKVLVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            complement(70059..70949)
                     /gene="alsR"
                     /locus_tag="POB34_000060"
                     /gene_synonym="rpiR"
     CDS             complement(70059..70949)
                     /gene="alsR"
                     /locus_tag="POB34_000060"
                     /gene_synonym="rpiR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="als operon DNA-binding transcriptional repressor
                     AlsR"
                     /protein_id="HDI5917087.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            71309..71758
                     /gene="rpiB"
                     /locus_tag="POB34_000061"
     CDS             71309..71758
                     /gene="rpiB"
                     /locus_tag="POB34_000061"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HDI5917088.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEVDGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
     gene            71827..72156
                     /gene="yjdP"
                     /locus_tag="POB34_000062"
     CDS             71827..72156
                     /gene="yjdP"
                     /locus_tag="POB34_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HDI5917089.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(72303..73061)
                     /gene="phnP"
                     /locus_tag="POB34_000063"
     CDS             complement(72303..73061)
                     /gene="phnP"
                     /locus_tag="POB34_000063"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HDI5917090.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVVPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(73063..73497)
                     /gene="phnO"
                     /locus_tag="POB34_000064"
     CDS             complement(73063..73497)
                     /gene="phnO"
                     /locus_tag="POB34_000064"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HDI5917091.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            complement(73484..74041)
                     /gene="phnN"
                     /locus_tag="POB34_000065"
     CDS             complement(73484..74041)
                     /gene="phnN"
                     /locus_tag="POB34_000065"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HDI5917092.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(74041..75177)
                     /gene="phnM"
                     /locus_tag="POB34_000066"
     CDS             complement(74041..75177)
                     /gene="phnM"
                     /locus_tag="POB34_000066"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HDI5917093.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CHARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            complement(75174..75854)
                     /gene="phnL"
                     /locus_tag="POB34_000067"
     CDS             complement(75174..75854)
                     /gene="phnL"
                     /locus_tag="POB34_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HDI5917094.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTIGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            complement(76031..76789)
                     /gene="phnK"
                     /locus_tag="POB34_000068"
     CDS             complement(76031..76789)
                     /gene="phnK"
                     /locus_tag="POB34_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HDI5917095.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(76786..77631)
                     /gene="phnJ"
                     /locus_tag="POB34_000069"
     CDS             complement(76786..77631)
                     /gene="phnJ"
                     /locus_tag="POB34_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HDI5917096.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            complement(77624..78688)
                     /gene="phnI"
                     /locus_tag="POB34_000070"
     CDS             complement(77624..78688)
                     /gene="phnI"
                     /locus_tag="POB34_000070"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HDI5917097.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEKNHG"
     gene            complement(78688..79272)
                     /gene="phnH"
                     /locus_tag="POB34_000071"
     CDS             complement(78688..79272)
                     /gene="phnH"
                     /locus_tag="POB34_000071"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HDI5917098.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(79269..79721)
                     /gene="phnG"
                     /locus_tag="POB34_000072"
     CDS             complement(79269..79721)
                     /gene="phnG"
                     /locus_tag="POB34_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HDI5917099.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRYFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(79722..80447)
                     /gene="phnF"
                     /locus_tag="POB34_000073"
     CDS             complement(79722..80447)
                     /gene="phnF"
                     /locus_tag="POB34_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HDI5917100.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(80468..81247)
                     /gene="phnE"
                     /locus_tag="POB34_000074"
     CDS             complement(80468..81247)
                     /gene="phnE"
                     /locus_tag="POB34_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDI5917101.1"
                     /translation="MQTITIAPSKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(81309..82325)
                     /gene="phnD"
                     /locus_tag="POB34_000075"
     CDS             complement(81309..82325)
                     /gene="phnD"
                     /locus_tag="POB34_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI5917102.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKMIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(82350..83138)
                     /gene="phnC"
                     /locus_tag="POB34_000076"
     CDS             complement(82350..83138)
                     /gene="phnC"
                     /locus_tag="POB34_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDI5917103.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSSKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            complement(83271..83714)
                     /gene="yjdN"
                     /locus_tag="POB34_000077"
     CDS             complement(83271..83714)
                     /gene="yjdN"
                     /locus_tag="POB34_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HDI5917104.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAMPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(83874..84209)
                     /locus_tag="POB34_000078"
     CDS             complement(83874..84209)
                     /locus_tag="POB34_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HDI5917105.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            84611..86839
                     /gene="crfC"
                     /locus_tag="POB34_000079"
     CDS             84611..86839
                     /gene="crfC"
                     /locus_tag="POB34_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HDI5917106.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELVNNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEMRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSAVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            86836..87714
                     /locus_tag="POB34_000080"
     CDS             86836..87714
                     /locus_tag="POB34_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HDI5917107.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGVFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLA"
     gene            87978..89480
                     /gene="proP"
                     /locus_tag="POB34_000081"
     CDS             87978..89480
                     /gene="proP"
                     /locus_tag="POB34_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HDI5917108.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            89592..89681
                     /gene="pmrR"
                     /locus_tag="POB34_000082"
     CDS             89592..89681
                     /gene="pmrR"
                     /locus_tag="POB34_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HDI5917109.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(89657..90757)
                     /gene="pmrB"
                     /locus_tag="POB34_000083"
     CDS             complement(89657..90757)
                     /gene="pmrB"
                     /locus_tag="POB34_000083"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HDI5917110.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(90758..91426)
                     /gene="basR"
                     /locus_tag="POB34_000084"
     CDS             complement(90758..91426)
                     /gene="basR"
                     /locus_tag="POB34_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HDI5917111.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMSGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(91423..93066)
                     /gene="eptA"
                     /locus_tag="POB34_000085"
     CDS             complement(91423..93066)
                     /gene="eptA"
                     /locus_tag="POB34_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HDI5917112.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            complement(93170..94507)
                     /gene="adiC"
                     /locus_tag="POB34_000086"
     CDS             complement(93170..94507)
                     /gene="adiC"
                     /locus_tag="POB34_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HDI5917113.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(94644..95405)
                     /gene="adiY"
                     /locus_tag="POB34_000087"
     CDS             complement(94644..95405)
                     /gene="adiY"
                     /locus_tag="POB34_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HDI5917114.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(95730..97997)
                     /gene="adiA"
                     /locus_tag="POB34_000088"
     CDS             complement(95730..97997)
                     /gene="adiA"
                     /locus_tag="POB34_000088"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HDI5917115.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPMAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(98196..99104)
                     /gene="melR"
                     /locus_tag="POB34_000089"
     CDS             complement(98196..99104)
                     /gene="melR"
                     /locus_tag="POB34_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HDI5917116.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            99387..100742
                     /gene="melA"
                     /locus_tag="POB34_000090"
     CDS             99387..100742
                     /gene="melA"
                     /locus_tag="POB34_000090"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDI5917117.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALHDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            100845..102266
                     /gene="melB"
                     /locus_tag="POB34_000091"
     CDS             100845..102266
                     /gene="melB"
                     /locus_tag="POB34_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HDI5917118.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(102405..103034)
                     /locus_tag="POB34_000092"
     CDS             complement(102405..103034)
                     /locus_tag="POB34_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HDI5917119.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(103157..104803)
                     /gene="fumB"
                     /locus_tag="POB34_000093"
     CDS             complement(103157..104803)
                     /gene="fumB"
                     /locus_tag="POB34_000093"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDI5917120.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(104881..106221)
                     /gene="dcuB"
                     /locus_tag="POB34_000094"
     CDS             complement(104881..106221)
                     /gene="dcuB"
                     /locus_tag="POB34_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HDI5917121.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(106792..107511)
                     /gene="dcuR"
                     /locus_tag="POB34_000095"
     CDS             complement(106792..107511)
                     /gene="dcuR"
                     /locus_tag="POB34_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HDI5917122.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(107508..109139)
                     /gene="dcuS"
                     /locus_tag="POB34_000096"
     CDS             complement(107508..109139)
                     /gene="dcuS"
                     /locus_tag="POB34_000096"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HDI5917123.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            109320..109550
                     /locus_tag="POB34_000097"
     CDS             109320..109550
                     /locus_tag="POB34_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDI5917124.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            109562..109834
                     /locus_tag="POB34_000098"
     CDS             109562..109834
                     /locus_tag="POB34_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI5917125.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            110061..110357
                     /gene="ghoS"
                     /locus_tag="POB34_000099"
     CDS             110061..110357
                     /gene="ghoS"
                     /locus_tag="POB34_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDI5917126.1"
                     /translation="MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            110385..110558
                     /gene="ghoT"
                     /locus_tag="POB34_000100"
     CDS             110385..110558
                     /gene="ghoT"
                     /locus_tag="POB34_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HDI5917127.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(110677..112194)
                     /gene="lysS"
                     /locus_tag="POB34_000101"
     CDS             complement(110677..112194)
                     /gene="lysS"
                     /locus_tag="POB34_000101"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDI5917128.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(112431..113888)
                     /gene="dtpC"
                     /locus_tag="POB34_000102"
     CDS             complement(112431..113888)
                     /gene="dtpC"
                     /locus_tag="POB34_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HDI5917129.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMVKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(113947..116094)
                     /gene="cadA"
                     /locus_tag="POB34_000103"
     CDS             complement(113947..116094)
                     /gene="cadA"
                     /locus_tag="POB34_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HDI5917130.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(116174..117508)
                     /gene="cadB"
                     /locus_tag="POB34_000104"
     CDS             complement(116174..117508)
                     /gene="cadB"
                     /locus_tag="POB34_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HDI5917131.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(117874..119412)
                     /gene="cadC"
                     /locus_tag="POB34_000105"
     CDS             complement(117874..119412)
                     /gene="cadC"
                     /locus_tag="POB34_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HDI5917132.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(120029..120104)
                     /locus_tag="POB34_000106"
     tRNA            complement(120029..120104)
                     /locus_tag="POB34_000106"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(120069..120071),aa:Phe,seq:gaa)
     gene            complement(120211..120786)
                     /locus_tag="POB34_000107"
     CDS             complement(120211..120786)
                     /locus_tag="POB34_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI5917133.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(120823..122520)
                     /gene="dsbD"
                     /locus_tag="POB34_000108"
     CDS             complement(120823..122520)
                     /gene="dsbD"
                     /locus_tag="POB34_000108"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HDI5917134.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(122496..122834)
                     /gene="cutA"
                     /locus_tag="POB34_000109"
     CDS             complement(122496..122834)
                     /gene="cutA"
                     /locus_tag="POB34_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HDI5917135.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(122950..124251)
                     /gene="dcuA"
                     /locus_tag="POB34_000110"
     CDS             complement(122950..124251)
                     /gene="dcuA"
                     /locus_tag="POB34_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HDI5917136.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFLLGSFML"
     gene            complement(124369..125805)
                     /gene="aspA"
                     /locus_tag="POB34_000111"
     CDS             complement(124369..125805)
                     /gene="aspA"
                     /locus_tag="POB34_000111"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDI5917137.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            126142..126618
                     /gene="fxsA"
                     /locus_tag="POB34_000112"
     CDS             126142..126618
                     /gene="fxsA"
                     /locus_tag="POB34_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HDI5917138.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            complement(126634..127890)
                     /gene="yjeH"
                     /locus_tag="POB34_000113"
     CDS             complement(126634..127890)
                     /gene="yjeH"
                     /locus_tag="POB34_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HDI5917139.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            128166..128459
                     /locus_tag="POB34_000114"
     CDS             128166..128459
                     /locus_tag="POB34_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDI5917140.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            128503..130149
                     /gene="groL"
                     /locus_tag="POB34_000115"
     CDS             128503..130149
                     /gene="groL"
                     /locus_tag="POB34_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDI5917141.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            130287..130640
                     /locus_tag="POB34_000116"
     CDS             130287..130640
                     /locus_tag="POB34_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HDI5917142.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(130833..131702)
                     /locus_tag="POB34_000117"
     CDS             complement(130833..131702)
                     /locus_tag="POB34_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HDI5917143.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGNKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLVGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(132097..133125)
                     /gene="epmB"
                     /locus_tag="POB34_000118"
     CDS             complement(132097..133125)
                     /gene="epmB"
                     /locus_tag="POB34_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDI5917144.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            133167..133733
                     /gene="efp"
                     /locus_tag="POB34_000119"
     CDS             133167..133733
                     /gene="efp"
                     /locus_tag="POB34_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDI5917145.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            133785..133910
                     /gene="ecnA"
                     /locus_tag="POB34_000120"
     CDS             133785..133910
                     /gene="ecnA"
                     /locus_tag="POB34_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HDI5917146.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            134021..134167
                     /gene="ecnB"
                     /locus_tag="POB34_000121"
     CDS             134021..134167
                     /gene="ecnB"
                     /locus_tag="POB34_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HDI5917147.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            134342..134659
                     /gene="sugE"
                     /locus_tag="POB34_000122"
     CDS             134342..134659
                     /gene="sugE"
                     /locus_tag="POB34_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDI5917148.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(134656..135189)
                     /gene="blc"
                     /locus_tag="POB34_000123"
     CDS             complement(134656..135189)
                     /gene="blc"
                     /locus_tag="POB34_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDI5917149.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(135278..136411)
                     /locus_tag="POB34_000124"
     CDS             complement(135278..136411)
                     /locus_tag="POB34_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDI5917150.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(136474..136833)
                     /gene="frdD"
                     /locus_tag="POB34_000125"
     CDS             complement(136474..136833)
                     /gene="frdD"
                     /locus_tag="POB34_000125"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDI5917151.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            complement(136844..137239)
                     /gene="frdC"
                     /locus_tag="POB34_000126"
     CDS             complement(136844..137239)
                     /gene="frdC"
                     /locus_tag="POB34_000126"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDI5917152.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(137250..137984)
                     /gene="frdB"
                     /locus_tag="POB34_000127"
     CDS             complement(137250..137984)
                     /gene="frdB"
                     /locus_tag="POB34_000127"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HDI5917153.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(137977..139785)
                     /gene="frdA"
                     /locus_tag="POB34_000128"
     CDS             complement(137977..139785)
                     /gene="frdA"
                     /locus_tag="POB34_000128"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDI5917154.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            140110..141087
                     /gene="epmA"
                     /locus_tag="POB34_000129"
     CDS             140110..141087
                     /gene="epmA"
                     /locus_tag="POB34_000129"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDI5917155.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIDALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            141306..142808
                     /gene="yjeM"
                     /locus_tag="POB34_000130"
     CDS             141306..142808
                     /gene="yjeM"
                     /locus_tag="POB34_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HDI5917156.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGMTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTIFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(142959..146282)
                     /gene="mscM"
                     /locus_tag="POB34_000131"
     CDS             complement(142959..146282)
                     /gene="mscM"
                     /locus_tag="POB34_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HDI5917157.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(146304..147272)
                     /gene="psd"
                     /locus_tag="POB34_000132"
     CDS             complement(146304..147272)
                     /gene="psd"
                     /locus_tag="POB34_000132"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDI5917158.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(147369..148421)
                     /gene="rsgA"
                     /locus_tag="POB34_000133"
     CDS             complement(147369..148421)
                     /gene="rsgA"
                     /locus_tag="POB34_000133"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDI5917159.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            148516..>149008
                     /gene="orn"
                     /locus_tag="POB34_000134"
     CDS             148516..>149008
                     /gene="orn"
                     /locus_tag="POB34_000134"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDI5917160.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDI"
BASE COUNT        35769 a        37132 c        38842 g        37265 t
ORIGIN      
        1 tttgcgcagg ataccttccg cctgttcttg cttgccgcgt gacatcagcc agcgaggact
       61 ttctggcacg gtatacagca gcattaagaa cagcagtgca gggatacatt ccgaggcaaa
      121 catataacgc cagccgtcag tattcagcca gctggcgtcg ccggaacggg caataaaata
      181 gtttacgcag taaactaaaa gctgcccgaa aataatcgca aactggttaa aagagaccag
      241 tttcccgcga atatgagcag gagcaagttc cgcaatatac attggtgaaa gcattgaggc
      301 taaaccaacg ccaataccgc caataatgcg ataaataaca aattccggga cataacctgc
      361 cagataaaca ggcactgtgt tgtccgggtt tatagaggta aaaccaagtt ctggccaggc
      421 agaacctaca ccagaaataa aaaacaggac agcagcaatc ttaagtgaat cacgacgacc
      481 gaagcggcta ctgcaataac caccgagggc accgccgatg atgcaaccaa tcagagcgct
      541 ggccacgcaa aaccctaaca gggagttggc agcggattca cttaagtttt gtggagcaac
      601 aaagacggta ttgagtgact caacagtacc ggaaataacg gcggtgtcgt agccaaataa
      661 taaaccacct aatgtagcga ctaaggtaat cgaaaatata taactggaat tatactgggt
      721 attcattcag acctgcctta gaccattctg atcttttatc agtgcattga tgttggaatt
      781 gtcaggcagt taaggcaatt atcatttttt acaatgcgtt tacgtaatgt cttttctgtg
      841 atcttaattg tgataattat caaaaattga tgaaaacaat actattgccg tgactcagag
      901 cacgaaagag aattatcgta agtgggaaaa caaataacgt aaaaataata agttctatga
      961 tggaaatatt aaccggcgaa cgattcagat tgcagacgaa agaaaaaaag gcgctccgtg
     1021 gagcgccgaa taacagtcac aagttgggat aacgtaagtt gagggtgcag cggcataaca
     1081 ttggcagaac aacatcttta acctttcaca ccacctgccg tcaggccgtt gaccagccag
     1141 cgttgagcca gcaagaagac gatggtgatc ggtaatgcag acatcacggc agcggcggca
     1201 aagtcacccc acaggtagtt ttgcgggttg aggtattgct gcatccccac ggccagggtg
     1261 tagctgttta cgtcacgcag taacagcgac gcgaccggaa cttcagtaat ggcagcgata
     1321 aacgacagga tgaacaccac cgccagaatc ggtactgaca gcggcaacag gacaaggcgg
     1381 aaggcctgcc acggtgtcgc accatccagc gccgccgctt cttccagcga actgtcgatg
     1441 gtttcgaaat agcctttgat ggtccagacg tgcagcgcaa tcccacccag atacgcgaaa
     1501 attacgccgc cgtgagtatt caggccaatg aatggaatgt actcacccag acgatcaaac
     1561 aacgcataca acgcgaccag tgaaagtact gccgggaaca tctggaaaat cagcattcct
     1621 ttcagcagcg tcgctttgcc tggaaagcgc atacgggcga aagcgtaggc gcaggtggtg
     1681 gagagcgcca caatgccaat cgcggaaatc ccggcgacct ttaccgagtt ccacagccac
     1741 agcagtaccg ggaatggcgg tggcgtaatg cgaccatcag cctgttccac gctaaaacct
     1801 aatgccagtt tccagtgatc ccaggagatt tgctccggga tcaggctacc ggtcgcaaag
     1861 ttcccctggc gcagtgagat ggcgacgacc atcagcagcg ggaacataat cgccgcgata
     1921 aaaagtagca gtagcaggtg agtaataaac aaacgtgctt tttgcgattt cggttggacc
     1981 attgccattt ttgttatctc ccttaatcaa acttcattcg cgtggctttc aggttcacta
     2041 tcgccagcgc acccaccagc aggaagatca gcgtggctat tgctgctgcc agaccgaagt
     2101 cctgaccccc gccgccttca aaagcgatgc ggtaggtgta gttaacaagc aggtcggtat
     2161 aaccggctgg cgtggtcgtg ccaagacgat ccgggccgcc gttggttaac agttgaatca
     2221 gcacgaagtt gttaaagtta aaggcgaagc tggcgatcat cagcggcgtc agcggtttaa
     2281 tcagcagcgg cagcgtaatc ttaaagaagt tctggaaggg acctgcacca tccattgctg
     2341 aggcttcata caaatcgtcc ggaatcgctt tcagcaagcc catgcagagg atcatcatgt
     2401 acggataacc cagccaggta ttgacgataa tcagcatcgt tcgggcggtg gtcggatcgc
     2461 tgaaccaggc gggcttcacg ccaaacagcg cgctcaacat catgttgatc tcaccgaagc
     2521 tctggttaaa caaccctttg aaaatcaaga ttgaaatgaa tgatggcacc gcgtagggca
     2581 gaatcagcag gacgcgatag accgctttgc cgcgcaacgc ttcccactgc accagacacg
     2641 ccagaaccat gccgaccgcc accgttaaaa agacagtgat cagcgagaac accacggtcc
     2701 agacgaaaat ggcgaggaac ggtttctgaa tgccttcgtc ggtgaagacg cgggtaaagt
     2761 ttttccagcc agtagtcacg gtgtaaccgg ggcttagctt ttcatcgccc cagttgccgt
     2821 cggcggtaat ggactggtaa aagccaattt ggttattcgg acgatatttc acgccgctct
     2881 gattattcgt caacgtgccg tcaccgtcga gtgtgtagag cggctgcgtg ccagaaaact
     2941 ggcgcaggga gctcatcatc actttgttgc catccggcag aatggcggta atgtcactca
     3001 gcgcctgacg attctgggta atcacgcgca gatttgcgcg ttcgccttcg ggctgggcgg
     3061 tcgtttcttt cagttgcagt ttttgctcgc cgccaaattt aaaagcgtcg gagaggtaat
     3121 ttttgccggt ttcgccgtcg ctgagggcta gttgccactc atcgcccgcc gggtaaagac
     3181 caaaattata ggttttgcct gcttgccagg agcgatctaa caacacttcc tgcgcacgtt
     3241 caaaagtcag ctggttagtg ctgctgtagt tggtgaaggc aatggcgatg gtgcagacca
     3301 gagggaagag gacgaataat cccattccgg ccattcccgg gtaaacatag cgccaggcgt
     3361 aggctttacg attggcgaaa atatacagcc ccgctgaact caatatcagc gtggtaatgg
     3421 caaacaggta ttccccttgt gcgtacatta aaacaacaag gtaacccacc agcaggccga
     3481 gcagacctag cactgaccat ttcagcgcgt cgctttgcca ccaatgtttc tttttaatga
     3541 catccatggg gttcttcctc attccaggac ggataaggct ttcacgcctt atccgacaac
     3601 aactgcctga tgcgacactg atgcgtctta tcaggcctac atacgtttcg gttttgtagg
     3661 ccggacaagg cgttcacgcc gcatccggca tttcacagca ttacttggtg atacgagtct
     3721 gcgcgtcttt cagggcttca tcgacagtct gacgaccgct ggcggcgttg atcaccgcag
     3781 tacgcacggc ataccagaaa gcggacatct gcgggatgtt cggcatgatt tcacctttct
     3841 gggcgttttc catggtggct gcaatacgtg gatctttcgc caactcttcc tcgtaagact
     3901 tcagcgctac ggcacccagc ggtttgtctt tattaaccgc ttccagacct tcgtcagtca
     3961 gcagatagtt ttcgaggaac tctttcgcca gctctttgtt cggactggcg gcgttaatac
     4021 cggcgctcag cacgccaacg aacggtttag atggttgacc cttgaaggtc ggcagtaccg
     4081 ttacaccata attcactttg ctggtgtcga tgttggacca tgcccacggg ccgttgatgg
     4141 tcatcgctgt ttcgccttta ttaaaggcag cttctgcgat ggagtaatcg gtgtctgcat
     4201 tcatgtgttt gtttttaatc aggtcaacca ggaaggtcag acccgctttc gcgccagcgt
     4261 tatccacgcc cacgtcttta atgtcgtatt tgccattttc atacttgaac gcataacccc
     4321 cgtcagcagc aatcagcggc caggtgaagt acggttcttg caggttgaac atcagcgcgc
     4381 tcttaccttt cgctttcagt tctttatcca gcgccgggat ctcttcccag gtttttggtg
     4441 ggttcggcag cagatctttg ttataaatca gcgataacgc ttcaacagcg atcgggtaag
     4501 caatcagctt gccgttgtaa cgtacggcat cccaggtaaa cggatacagc ttgtcctgga
     4561 acgctttgtc cggggtgatt tcagccaaca ggccagattg agcgtagcca ccaaaacggt
     4621 cgtgcgccca gaagataatg tcagggccat cgccagttgc cgcaacctga gggaatttct
     4681 cttccagttt atccggatgc tcaacggtga ctttaattcc ggtatctttc tcgaatttct
     4741 taccgacttc agcgagaccg ttatagcctt tatcgccgtt aatccagatt accagtttac
     4801 cttcttcgat tttggcgaga gccgaggcgg aaaacatcat cgtcgttaat gcggataatg
     4861 cgaggatgcg tgcacctgtt tttattttca taatctatgg tccttgttgg tgaagtgctc
     4921 gtgaaaacac ctaaacggac tctagtttct ttatacggca acctctttcc atcctccttg
     4981 cccctacgcc ccaccgttgc tttgtgtgat ctctgttaca gaattggcgg taatgtggag
     5041 atgcgcacat aaaatcgcca cgatttttgc aagcaacatc acgaaattcc ttacatgacc
     5101 tcggtttagt tcacagaagc cgtgttctca tcctcccgcc tcctccccca taaaaaagcc
     5161 agggggtgga ggatttaagc catctcctga tgacgcatag tcagcccatc atgaatgttg
     5221 ctgtcgatga caggttgtta caaagggaga agggcatggc gagcgtacag ctgcaaaatg
     5281 taacgaaagc ctggggcgag gtcgtggtat cgaaagatat caatctcgat atccatgaag
     5341 gtgaattcgt ggtgtttgtc ggaccgtctg gctgcggtaa atcgacttta ctgcgcatga
     5401 ttgccgggct tgagacgatc accagcggcg acctgttcat cggtgagaaa cggatgaatg
     5461 acactccgcc agcagaacgc ggcgttggta tggtgtttca gtcttacgcg ctctatcccc
     5521 acctgtcagt agcagaaaac atgtcatttg gcctgaaact ggctggcgca aaaaaagagg
     5581 tgattaacca acgcgtcaac caagtggcgg aagtgctgca actggcgcat ttgctggatc
     5641 gcaaaccgaa agcgctctct ggcggtcagc gtcagcgtgt ggcgattggc cgtacgctgg
     5701 tggccgagcc aagcgtattt ttgctcgatg aaccgctctc caacctcgat gctgcactgc
     5761 gtgtgcaaat gcgtatcgaa atctcccgtc tgcataaacg cctgggccgc acaatgattt
     5821 acgtcaccca cgatcaggtc gaagcgatga cgctggccga caaaatcgtg gtgctggacg
     5881 ccggtcgcgt ggcgcaggtt gggaaaccgc tggagctgta ccactatccg gcagaccgtt
     5941 ttgtcgccgg atttatcggt tcgccaaaga tgaacttcct gccggtaaaa gtgaccgcca
     6001 ccgcaatcga tcaagtgcag gtggagctgc cgatgccaaa tcgtcagcaa gtctggctgc
     6061 cagttgaaag ccgtgatgtc caggttggag ccaatatgtc gctgggtatt cgcccggaac
     6121 atctactgcc gagtgatatc gctgacgtca tccttgaggg tgaagttcag gtcgtcgagc
     6181 aactcggcaa cgaaacccaa atccatatcc agatcccttc cattcgtcaa aacctggtgt
     6241 accgccagaa cgacgtggtg ttggtagaag aaggtgccac attcgctatc ggcctgccgc
     6301 cagagcgttg ccatctgttc cgtgaggatg gcactgcatg tcgtcgactg cataaggagc
     6361 cgggcgttta agcaccccac aaaacacaca aagcctgtca caggtgatgt gaaaaaagaa
     6421 aagcaatgac tcaggagata gaatgatgat tactctgcgc aaacttcctc tggcggttgc
     6481 cgtcgcagcg ggcgtaatgt ctgctcaggc aatggctgtt gatttccacg gctatgcacg
     6541 ttccggtatt ggctggacag gtagcggcgg tgaacaacag tgtttccaga ctaccggtgc
     6601 tcaaagtaaa taccgtcttg gcaacgaatg tgaaacttat gctgaattaa aattgggtca
     6661 ggaagtgtgg aaagagggcg ataagagctt ctatttcgac actaacgtgg cctattccgt
     6721 cgcgcaacag aatgactggg aagctaccga tccggccttc cgtgaagcaa acgtgcaggg
     6781 taaaaacctg atcgaatggc tgccaggttc caccatctgg gcaggtaagc gcttctacca
     6841 acgtcatgac gttcatatga tcgacttcta ctactgggat atttctggtc ctggtgccgg
     6901 tctggaaaac atcgatgttg gcttcggtaa actctctctg gcagcaaccc gctcctctga
     6961 agctggtggt tcttcctctt ttgccagcaa caatatttat gactatacca acgaaaccgc
     7021 gaacgacgtt ttcgatgtgc gtttagcgca gatggaaatc aacccgggcg gcacattaga
     7081 actgggtgtc gactacggtc gtgccaacct gcgtgataac tatcgtctgg ttgatggcgc
     7141 atcgaaagac ggctggttat tcactgctga acatactcag agtgtcctga agggctttaa
     7201 caagtttgtt gttcagtacg ctactgactc gatgacctcg cagggtaaag gtctgtcgca
     7261 gggttctggc gtcgcgtttg ataacgaaaa atttgcctac aatatcaaca acaacggtca
     7321 catgctgcgt atcctcgacc acggtgcgat ctccatgggc gacaactggg acatgatgta
     7381 cgtgggtatg taccaggata tcaactggga taacgacaac ggcaccaagt ggtggaccgt
     7441 tggtattcgc ccgatgtaca agtggacgcc aatcatgagc accgtgatgg aaatcggcta
     7501 cgacaacgtc gaatcccagc gcaccggcga caagaacaat cagtacaaaa ttaccctcgc
     7561 acaacaatgg caggctggcg acagcatctg gtcacgcccg gctattcgtg tcttcgcaac
     7621 ctacgccaag tgggatgaga aatggggtta tgactacaac ggcgatagca aggttaaccc
     7681 gaactacggc aaagccgttc ctgctgattt caacggcggc agcttcggtc gtggcgacag
     7741 cgacgagtgg accttcggtg cccagatgga aatctggtgg taatagcaaa acctgggccg
     7801 gataaggcgt ttacgccgca ttcgacaacc aacgcctgat gcgacgcttg cgcgtcttat
     7861 caggcctaca acggctgtca aatgtaggcc ggataaggcg tttacgccgc atccggcata
     7921 aaaacaggtt gtcattatct gaaaggggcg aaagcccctc tgattatcgg gtttagcgcg
     7981 ctattgcctg gctaccgctg agctccagat tttgaggtga aaacaatgaa aatgaataaa
     8041 agtctcatcg ccctctgttt atcagcaggg ttactggcaa gcgcgcctgg aatcagcctt
     8101 gccgatgtta actacgtacc gcaaaacacc agcgacgcgc cagccattcc atctgctgcg
     8161 ctgcaacaac tcacctggac accggtcgat caatctaaaa cccagaccac ccaactggcg
     8221 accggcggcc aacaactgaa cgttcccggc atcagtggcc cggttgctgc gtacagcgtc
     8281 ccggcaaaca ttggcgaact gaccctgacg ctgaccagcg aagtgaacaa acaaaccagc
     8341 gtttttgcgc cgaacgtgct gattcttgat cagaacatga ccccatcagc cttcttcccc
     8401 agcagttatt tcacctacca ggaaccaggc gtgatgagtg cagatcggct ggaaggcgtt
     8461 atgcgcctga caccggcgtt ggggcagcaa aaactttatg ttctggtctt taccacggaa
     8521 aaagatctcc agcagacgac ccaactgctc gacccggcta aagcctatgc caagggcgtc
     8581 ggtaactcga tcccggatat ccccgatccg gttgctcgtc ataccaccga tggcttactg
     8641 aaactgaaag tgaaaacgaa ctccagctcc agcgtgttgg taggaccttt atttggttct
     8701 tccgctccag ctccggttac ggtaggtaac acggcggcac cagctgtggc tgcacccgct
     8761 ccggcaccgg tgaagaaaag cgagccgatg ctcaacgaca cggaaagtta ttttaatacc
     8821 gcgatcaaaa acgctgtcgc gaaaggtgat gttgataagg cgttaaaact gcttgatgaa
     8881 gctgaacgcc tgggatcgac atctgcccgt tccaccttta tcagcagtgt aaaaggcaag
     8941 gggtaattac gccccacagt gctgattttg caacaactgg tgcgtctcct ggcgcacctt
     9001 tttttatgct tccttcctgg gatatgagcg attttttata gtaactcact tcttcttcac
     9061 taagaatatc cattatctca atgccttatc agagattctt ttcctttcgc cggtagtgtc
     9121 tggacattca ggctactttt ccaggttatt ttatttctgt tatgcagagg ttttatgata
     9181 agtcatatcc taaattctgg cggcaataac tctttgatga aacatgatgt ggtgcaagga
     9241 aataatatag tagatcttga tttactacgt aatttcaatg gggtgccagg tttaaacaga
     9301 gataacttta tttatatcag caatattttt tcaaatataa aacaacggaa cgaaaaaatc
     9361 atgcaataaa tatgtttcgt gaagtctcaa tcagtaatga tactataagt gtaaaattct
     9421 acagaaatga aaaaaattga atgcgcttgc aattttctga tggataaaga tgcgcagggg
     9481 tatatcgacc tgtctgattt ggatttaaca agttgtcatt ttaaaggtga cgttatatcg
     9541 aaggtgtctt ttttatcatc aaatctacaa catgtaacat tcgaatgtaa agaaattggg
     9601 gattgcaatt ttactactgc aatagttgat aatgtcatat ttagatgtcg acgtttacac
     9661 aatgtgattt ttatcaaagc gagtggtgaa tgtgtcgatt tcagcaaaaa tattcttgat
     9721 acagttgact tctcgcagag tcaacttact cagagtaatt ttcgcgaata tcagattaga
     9781 aattcaaact tcgataattg ttatctttac gcttcgcact tcaccagagc agagtttctg
     9841 tctgccaaag aaatatcatt tattaaatcg aatatgacag ctgttatgtt tgatcatgtg
     9901 cgaatatcga cagggaattt taaagattgc attacagaac aattggaatt aactattgat
     9961 tattcagata tatttgggaa tgaagatctc gatggttata tcaataacat tataaaaatg
    10021 attgatacat tgccagataa tgcaatgata ttgaaatccg ttctggcagt aaaactggtt
    10081 atgcaattaa aaattcttaa tattgttaat aaaaacttta ttgagaatat gaagaaaaca
    10141 tttagccatt gtccttatat aaaagatcca attatacgca gttatatcca ttctggtgaa
    10201 gataacaagt tcgatgattt tatgcgtcaa catcgattca gcaaggtgga tttcgatacc
    10261 caacagatga tccattttat taacaggttt aatatgaata aagggctgat tgataaaaat
    10321 aacaattttt ttatccaact tatcgatcag gccttacgat caacggatga tatgatcaaa
    10381 gcaaatgcct ggtatcttta taaagagtgg attcgtagtg atgatgtttc acctatattt
    10441 atagaaactg aagaaaagtt aagaaccttt aacactaata aattaacacg aaacgataat
    10501 atatttatcc tgttctcctc tgtcgatgat gggcctgtta tggtggtaag ctcccagcgc
    10561 ttacatgata tgttgaatcc tacaaaagat accaattgga attccacgta tatctacaaa
    10621 tccagacatg agatgttgcc tgttaatctt actcaggaaa cacttttcag ctccaaatct
    10681 caggataaac atgcgctttt ccccattttt actgcgagtt ggcgagctca tcgtataatg
    10741 aataagggtg tttaagtaaa ggaaaacatc accgttcctg gcatcctggg cggtgatgcc
    10801 cctatggttg ccctctccag cgcgggcatc ggcaaagcgt aaggttcaac agcgttttac
    10861 cacttcatgc gattgttgcg tttttgttgc gtattagatc acttaatttg ctttacatct
    10921 cccgtaaaca cttttctgcg atacaatgcc tttacgttat gtaacggaga gttcggcatg
    10981 tcacaccccg cgttaacgca actgcgtgcg ctgcgctatt ttaaagagat ccctgccctg
    11041 gaaccgcaac tgctcgactg gctgttgctg gaggattcca tgacaaaacg ttttgaacag
    11101 cagggaaaaa cggtaagcgt gacgatgatc cgcgaagggt ttgtcgagca gaatgaaatc
    11161 cccgaagaac tgccgctgct gccgaaagag tctcgttact ggttacgtga aattttgtta
    11221 tgtgccgatg gtgaaccgtg gcttgccggt cgtaccgtcg ttcctgtgtc aacgttaagc
    11281 gggccggagc tggcgttaca aaaattgggt aaaacgccgt taggacgcta tctgttcaca
    11341 tcatcgacat taacccggga ctttattgag ataggccgtg atgccggact gtgggggcga
    11401 cgttcccgcc tgcgattaag cggtaaaccg ctgttgctaa cagaactgtt tttaccggcg
    11461 tcaccgttgt actaagagga aaaaaatatg gagtggagtc tgacgcagaa taagctgctg
    11521 gcgtttcatc gcttaatgcg tacggataag ccaattggcg cgttactgct gctctggcca
    11581 acattatggg cgttgtgggt ggcgacaccg ggcgttcctc agctctggat cctggcggta
    11641 tttgtcgcgg gtgtctggct gatgcgcgct gctggatgtg tggtgaatga ttatgctgac
    11701 cgaaagtttg atggtcatgt taagcgcacg gcgaaccgac cacttcccag cggcgcggta
    11761 acagagaaag aggcgcgcgc gctgtttgta gtgctggtac tgatttcgtt tttactggta
    11821 ctgacgctga atacgatgac cattctgttg tcgattgccg ctctggcgtt ggcgtgggtg
    11881 tacccgttta tgaagcggta tacccatctc ccgcaagtgg tgctgggcgc ggcgtttggc
    11941 tggtcgattc caatggcttt tgccgctgtg agtgagtcgg tgccattgag ttgctggtta
    12001 atgttcctcg ccaatattct ctgggcggtg gcttacgaca cgcagtatgc gatggttgac
    12061 cgcgatgatg atgtgaagat tggcattaaa tccacggcaa tcctgttcgg ccaatacgat
    12121 aaattgatta ttggtatttt gcagattggc gtactggcac tgatggcgat catcggtgag
    12181 ttaaatggct taggctgggg atattactgg tcaattgtgg tggctggcgc gctgtttgtt
    12241 tatcaacaaa aactgattgc caaccgcgag cgtgaagcct gctttaaagc atttatgaat
    12301 aataactatg ttggtctggt actgttttta ggactggcaa tgagttactg gcatttctga
    12361 tgatgtaaaa aagccggatg atcatccggc tttcttctgg gttgccttat gcgcggcgct
    12421 tctcaggcct acacaacaca tcgcaattta ttgaatttgc agattatgga aggccggata
    12481 aggcgttttc gccgcatccg gcaattctct ctgattaccc ttcgccctgc gtcgcactct
    12541 caatcgtcaa acgcacgtct gatgtaatca actccgccag caactgataa accttcatcg
    12601 tttctgccgg ttcggcgtcg ccgctatcgc tgatataccc ttcttcacgc agcgtcagca
    12661 ccagagaact gaacaccgcc ttgtcgaaga actccggcgc gttgatgccg tgcagcacgg
    12721 agagacgttg cgcgacggtg cggctctctt tctccagcgt accgcggttg atcgacgggt
    12781 tggcactcaa caaccagaag gtgatggcat aacgttgcag cgtttcgcgc gcgcctgcgg
    12841 ccagcagctg tagggtgcga gaatgcgccg ggttgatatg caactcatca tcttgcaggg
    12901 taatcagccc ctgacgttgc atctcatttg ccagcgcatc aataacgtcc ggcaactcgt
    12961 cgcgatccca gcgcaggaac agctccgctt tcagcattgg gtaaagcaca ttgacgtgct
    13021 ccatcaatac gtcgcgggag atgtggcgat gctgggtgac aattgccgcc atcagcgaag
    13081 gcagcaccaa catatgcgca atgttgttgc gatagtaggt catcagcacc gcttgctcgc
    13141 gtggcagaat gatgatgtcg ccgatagtgt ctttctcgac ttcaaacttg ttcatttgca
    13201 gcgcgtgatc gataagctcg ctggcgctgg ctgaaggaac ggtagagtcc gtggagtaag
    13261 gtacgttgcg catcagatcc aggtagcagt tgagttgctc ggttaactgc tcgcgggtga
    13321 gtgagcgctg acgtgatgcc agaagcgcag tacaacacag gttcatggcg tttgccgcgc
    13381 ctgcgttgtt aatgcgtacc atcagatcgg cagcaatatt attgaccgtc ggcgttaacc
    13441 aggccggacg caccgcttcg atgggatcga tagattcacg ccagtctggt acgtgctggt
    13501 taaggtaggt catcaacggc attggttcac cgaagttgac gtaaccctga ccgagattac
    13561 gcagcttgct taaaccgcgc agcatctgcg gcaggctctc tttctctttc gtcgcgccgc
    13621 gcagttcttt ggcgtaagta cccacttcca tgacgtgctc ataaccgata tagatcggaa
    13681 tcagcgtaat cggacgcgtg ccgccacgca gcatcgcctg aatggtcatc gacagcgtac
    13741 cggttttcgg atccagcaaa cgccccgtgc gggaacgacc gccttccacg aagtactcga
    13801 cggaataacc acggctgaac agttcgccga gatactcacg gaaaacggtg gaataaagtt
    13861 tattgccttt aaaagtacgg cgaataaaga acgcccccag acggcggaaa atcggcccgg
    13921 ccggccagaa gttcaggttg atcccggcgg cgatatgcgg cggcaccagc ccctggtggt
    13981 acagcacgta agaaagtagc aggtagtcca tgtgactgcg gtggcaaggc acatacacca
    14041 gctcatgacc gtcgtgggcc agctggcgaa cgcgctcggc gttatggacg ttgatgccct
    14101 gataaagtcg gttccaggtg aagcccagaa tgcggtcagt caggcggatc atctcgtaag
    14161 agaaattcgc cgcaatctct tccatcagcg caatcgcgtt ctgctgcgct ttttcatggg
    14221 agattttttt gctgcgcgct tcatcttcta ccgctttggc aatggcgcgg gaggcgagca
    14281 gcttattaaa cagatcctga cgagcaggca gacgtgggcc cacagcagcc agacgttgac
    14341 gggcaaagtg catacgcgcc acgcgcgcca gtttctgtgc gatagtttta tccgtgccgt
    14401 gttcatccgc catacggcgc agcgaaactg acggcgagaa acgcacaaaa ctgtcgcgac
    14461 cgagccacag tacggcgaaa aatttctgta cgccgttaag catacgcagc ggcgggttta
    14521 cttcgccttt ttcacgcccc ggcgcgcgac caaacatcac cgatactggc accatctgca
    14581 catccagatt tgggttgcta cggtgcaaat cgagatagtc gtggaacagc ttaatagact
    14641 cttctttcgg cgtgtaatag gtgaatacac gcggcccgcc gtgaatgaac acatagcgcg
    14701 gcagtagcgt gccgtcgatt tccagcggct ctaacgggtc aggcaagtca tgagccagac
    14761 actgggcgcg taacgtcagc aaatctgctt tcgagttgta cggtaaaacg tacataattg
    14821 gacgagaagt atccagcccc agttccgggg caggatctgc cggaatagac ttgcttttta
    14881 ccaggatgct taatggtaaa ttcagtaatt tgtagtaaat tcgtggccag ccggacataa
    14941 acgatgtaaa gcctctggtt aataatgcaa atgcgcggca aggatagcag aaagtcatgg
    15001 gaaattctgt ggtatccgct catgtttcgc gcggcgttac gcaaacccga atcatcggat
    15061 ttaacggtac actgatattg acgctcataa tgtaaaaagg ttctttcaat ggccaataat
    15121 accactggat tcacccgaat tattaaagct gctggctatt cctggaaagg tttacgcgct
    15181 gcatggatca acgaagcggc attccgtcag gaaggcgtag cggtattgtt ggcggtggtc
    15241 atcgcctgct ggctggatgt ggacgcaatt acccgcgtgc tgcttatcag ctccgtgatg
    15301 ctggtgatga ttgtggaaat cctcaatagc gccatcgaag cagtggttga ccgaattggc
    15361 tctgaatacc atgagctttc cggacgcgca aaagatatgg gatccgccgc ggtgctgatt
    15421 gccattatcg tcgccgtgat tacctggtgc attctgttat ggtcgcattt tggataaccc
    15481 ttccagattt cgataaatct ctggtttatt gtgcagttta tggttccaaa atcgcctttt
    15541 gctgtatata ctcacagcat aactgtatat acacccaggg ggcggaatga aagcgttaac
    15601 ggccaggcaa caagaggtgt ttgatctcat ccgtgatcac atcagccaga caggtatgcc
    15661 gccgacgcgt gcggaaatcg cgcagcgttt ggggttccgt tccccaaacg cggctgaaga
    15721 acatctgaag gcgctggcac gcaaaggcgt tattgaaatt gtttccggcg catcacgcgg
    15781 gattcgtctg ttgcaggaag aggaagaagg gttgccgctg gtaggtcgtg tggctgccgg
    15841 tgaaccgctt ctggcgcaac agcatattga aggtcattat caggtcgatc cttccttgtt
    15901 caagccgaat gctgatttcc tgctgcgcgt cagcgggatg tcgatgaaag atatcggcat
    15961 tatggatggc gacttgctgg cagtgcataa aactcaggat gtacgtaacg gtcaggtcgt
    16021 tgtcgcacgt attgatgacg aagttaccgt taagcgcctg aaaaaacagg gcaataaagt
    16081 cgaactgttg ccagaaaata gcgagtttaa accaattgtc gttgaccttc gtcagcagag
    16141 cttcaccatt gaagggctgg cggttggggt tattcgcaac ggcgactggc tgtaacatat
    16201 ctctgagacc gcgatgccgc caggcgtcgc ggtttgtttt tcatctctct tcatcaggct
    16261 tgtctgcatg gcattcctca cttcatctga taaagcactc tggcatctcg ccttacccat
    16321 gattttctcc aatatcaccg ttccgttgct gggactggtc gatacggcgg taattggtca
    16381 tcttgatagc ccggtttatt tgggcggcgt ggcggttggt gcaacggcga ccagctttct
    16441 ctttatgctg ttgctgtttt tacgcatgag caccaccggg ctgactgcgc aggcttatgg
    16501 tgccaaaaat cctcaggcat tagcccgtgc gctggtgcaa ccgttgctgt tggcgttagg
    16561 ggctggggcg ttaattgcgc tgctgcgtac accgattatc gatctggcgc tgcatattgt
    16621 tggcggtagt gaagcggtcc tggaacaggc gcggcgcttt cttgaaatcc gctggttaag
    16681 cgctccggcg tcgctggcga atctggtatt actcggttgg ttactcggcg tgcaatatgc
    16741 ccgtgcgcca gtaattttgt tagtggtcgg caatatcctc aacattgtgc tggatgtctg
    16801 gctggtgatg gggctgcata tgaacgtgca gggcgcggcg ctggcgacgg ttattgcgga
    16861 atatgcaaca ttgctgattg gtctgctaat ggtgcgtaaa atcctcaaac tacgcggaat
    16921 ttccggcgaa atgctgaaaa ctgcctggcg aggaaacttc cgtcgcttgc tggcgcttaa
    16981 ccgcgatatc atgctgcgtt cgctgttgtt gcaactctgt ttcggcgcga tcaccgtact
    17041 tggtgcgcga ctggggagtg acattatcgc tgttaacgcg gttctgatga cgctactcac
    17101 ctttaccgcc tatgcgcttg atggttttgc ctacgcggtt gaagcgcact ccggtcaggc
    17161 atacggtgcg cgcgacggta gccagttgct ggatgtatgg cgggcagcgt gccgccagtc
    17221 ggggattgta gcgttactgt tttcggtggt ttatttgctg gcaggggaac acatcattgc
    17281 gttgctgacg tcgttaaccc agatccagca gctggctgac cgctatctta tctggcaggt
    17341 gattttgccg ttggttggcg tctggtgtta tctgctggac ggcatgttta taggcgcaac
    17401 gcgtgccgcc gaaatgcgta acagtatggc ggtggccgcc gcaggttttg cgctgacgct
    17461 ccttacgctg ccgtggctgg gtaatcatgg tttgtggctg gcattaaccg tctttctggc
    17521 gttgcgcggg ctttctctgg cggctatctg gcggcgtcac tggcgcaacg gtacctggtt
    17581 tgccgcaacg tgacggttaa aaattctgaa taaattatcc taagccaaat tgctgactac
    17641 acttaatctc acgttcagaa gaaaagtgaa cgtactctca ttcacaacct aacgatgagg
    17701 tcttgattat gaataaagat gaagccggcg gtaactggaa acagtttaaa ggtaaagtga
    17761 aagagcaatg gggcaaactg accgatgatg atatgacgat cattgaaggt aaacgtgatc
    17821 aactggtcgg taaaatccag gaacgttatg gttatcagaa agatcaggca gaaaaagagg
    17881 tcgtggattg ggaaacccgc aatgaatatc gctggtaatt aatccctcct gcccgacgtg
    17941 tacaaggatg tacgccctct taacgcggtt tctttttcac ctgcacagag tgatcatgct
    18001 ggcactgttc aggatgacga cacgcttcca cttccacgca tgccgcacag agcccatgtg
    18061 cttcaatcac attatgccgc agggcaaacc ccatttttgc cgccagcgta tgcataatgt
    18121 cttccacgcc ttctgcacac tcttctttca ccgcgccgca gcgatcgcaa ataaacatgg
    18181 ctgacgtatg cgtgggctgg tcgaacagat gacagagcac ataactgttg gtggattcca
    18241 ccttatgcac aaaaccttgc tcaagcagaa aatccagcgc gcgataaacc gttggcggct
    18301 tggcttgcgg ttcagcttcg cgcagcaaat caagcagatc ataagcgctg atagcgccat
    18361 cctgcagact catcaggcgc aacacttcca ggcgctgtgg ggtcaggcgc acattacgct
    18421 gcgcgcagat tttttcagcc tgcgctaata actcctgcgt tgtggtcttt tccatcgggc
    18481 acctcaaagt gggggttaag aatgtcctta ctttaccatg ttccaggaaa aacaccgaga
    18541 acccttattg ttgccgtaat gttgattttc tgttttgtac gtaaggtgtt gtgttgcttg
    18601 gtcgtaccat tatcaacacg ataataatta ataattcatt tttaaataaa ttgcttcaag
    18661 ttaactcgct atttatttaa ctgggtttga tcgcatacct gtagttcaga aattatgagc
    18721 gatttttgat agtgaccaga tggcaaaaca cttataagga ggacacggta cgacaaaaaa
    18781 ggtagatgaa tcatgttgaa gataataccc ggtgcaactg gatactttaa taaaacactc
    18841 aattcaaatc aattcgataa taaagatgca aacaaagata aattagataa tatagatgca
    18901 accaaagata aattagataa tagaggtaca atcaaaggta aattaaataa tatatatggt
    18961 aagtctattg attattcagc gctgcgtcat cgggatataa ttattgcaaa aatagatttg
    19021 tttattcaaa gaatcactca taatttatgg catgcacgta aaaaaaatgt gtttttaatt
    19081 gagcaaataa ctaatctcaa agtgtgggtc aataagtata ttgacgattg cactgatgag
    19141 gatttaaacg atcgtgactt tattgcatca gttgttgacc gggctatttt tcatttcgcg
    19201 attaatagta tatgtaatcc tggggataat aaagatgcga ctcccattga acgatgtact
    19261 tttgatgtag aaactaagaa tggccttccc tccacggttc agctatttta tgaggaatct
    19321 aaggataatg aacctttagc gaatatacat tttcaagcaa taggttctgg ttttttaacg
    19381 tttgttaatg cctgccagga acatgatgac aacagcttaa aattatttgc ttcgctgtta
    19441 atttcacttt catattctag tgcctacaca gatttatcag aaacagtgta tattaatgaa
    19501 aataatgaga gctacctgaa agctcagttt gaaaaattat ctcaacgtga tatgaagaag
    19561 tacctgggag agatgaagcg tctggctgat gggggagaaa tgaattttga tggctatctg
    19621 gataagatgt cacatctggt gaatgaagga acgctcgatc ctgatatttt aagcaaaatg
    19681 cgagatgctg caccacaatt aattagcttc gcgaagtcgt ttgacccaac ctcaaaggaa
    19741 gagattaaaa tacttacaga cacttctaaa ttaatttatg atttgttcgg ggttaaatcg
    19801 gagaaataat atgtgaagtt cttcgatagt atggaaggca ttatataaaa ggacccaata
    19861 tttattgggt tcttttttct ctatcaatgc tattagcagg gagatatatc accagagttt
    19921 aatgtgtgat tttttattta tcgtcgaacc tggattgttt atcattggcc ttaacaaagt
    19981 taacggctaa taagattatt tccatcactt cgtgagagct tcatgccttg agaggatctc
    20041 aattttcttt tgcaatgaga caggcgcttc ctgttgttat ggtatagtac cccgctattg
    20101 agtctcctga atagtgatgc tgaataacat aaccccatga tatatcgata aaataatctc
    20161 tacatttgaa aatgcacggt aattctgaaa tgcaaaaaat caaccaaacc agcgcaatgc
    20221 ctgaaaaaac tgacgttcac tggagtggtc ggtttagcgt tgcaccaatg ctcgactgga
    20281 cggacagaca ttgccgctat ttcttgcgtc tgctttcccg caatacgttg ctgtataccg
    20341 aaatggtgac cacaggggcg attattcacg gtaaaggtga ttacctggcg tacagtgaag
    20401 aagaacatcc ggtagcgttg caactcggcg gtagcgatcc ggcggcgctg gcacagtgtg
    20461 cgaagctggc agaagcgcgt ggatatgatg agatcaacct gaatgtcggc tgcccgtctg
    20521 accgggtgca gaacggcatg tttggtgcgt gtctgatggg taatgcgcag ctggttgccg
    20581 actgcgtgaa agcgatgcgc gatgtggtgt cgattccagt gacggtgaaa acgcgtattg
    20641 gcatcgatga ccaggacagc tatgaatttc tctgcgattt catcaacacc gtttccggca
    20701 aaggcgagtg tgagatgttt atcatccacg cacgtaaagc ctggctttcg gggttaagcc
    20761 cgaaagaaaa ccgtgaaatc ccgccgctcg attatccgcg tgtgtatcaa ctgaagcgtg
    20821 actttccgca tctgacaatg tcgattaacg gtggtatcaa gtcgctggaa gaggccaaag
    20881 cacacctgca acatatggat ggcgtgatgg tcgggcgcga ggcgtatcag aatccgggta
    20941 ttctggcggc ggtagaccgg gagatctttg gttcctcgga taccgatgcc gatccagtgg
    21001 cggtagtgcg cgccatgtat ccgtacattg agcgtgaact cagccagggg acgtatctcg
    21061 gccatattac ccggcatatg ttgggcttgt tccagggtat tcctggcgcg cggcagtggc
    21121 ggcgttattt aagtgaaaat gcccataaag cgggtgcaga cattaatgtg ctggaacacg
    21181 cgctcaaact ggtggcggat aagcgttaac ttttcaccaa aaagtagtca aattcaccac
    21241 gccctgcgca ccgtcgcggg gcattttgct gttaaatcaa tagattattt ttggcatgat
    21301 tcttgtaatg ccagcaagag atttcatatt tgggagagca tcatgctgga actacttttt
    21361 gtgtttggct tttttgtcat gctgatggtc accggcgttt cgttgctggg cattatcgcc
    21421 gcgctggttg tggcgacggc cattatgttc ctcggcggta tgctggcatt gatgattaag
    21481 ttgctgccgt ggttactact ggcgattgcg gtggtgtggg ttattaaggc gattaaagca
    21541 ccaaaagtgc cgaaatatca gcgttatgac cgctggcgtt actaagggat tgtgcggatg
    21601 atcacaacct aaggttttat ccttagaaca aaataggaat tgataatcaa atctgtcact
    21661 attgcgcctc taacagattc atcgtgctgt accctacata cagccgaact ataaaaagaa
    21721 agggcttccc aggtggaagc cctatttctt ttatggaatc agcaggctgg aaccttgcgt
    21781 cgcccggctt tccagaatct catgcgcacg ctgcgcatcc ttcagcggat atttctgctg
    21841 ctcggcgaca tcgaccttaa tcacaccgct ggcaatcaaa gagaacagtt cattactggc
    21901 ctcggttaat tcctcccgcg tggtgatata gccttgcagg gaagggcgtg tcacatacaa
    21961 cgagcctttt tgattgagaa tgcctaagtt cacaccggta accgcacctg atgagttgcc
    22021 aaaactgacc attaagccgc ggcgttgcag gcaatccagc gaccgttccc aggtgtctct
    22081 gcccacggaa tcgtacacta cgcgcacttt cttaccgccg gtgatctctt tcaatcgctc
    22141 gaccagattc tcttcacgat agttaataac ctgccacgcg ccggctttta gcgcgctctg
    22201 cgctttttgc gcggttccta cggtgccgat aagtttcgcg cccagggctt ttgcccactg
    22261 gcaggcaatt aagccaacgc cgccagccgc tgcgtggaac aggaactgtt catcgggttt
    22321 aatttcatag gttttgcgca gcagataata aaccgttaag cctttcagga aagatgctgc
    22381 agcttgttca aaagaaattg ccgcaggcag aatcgccgct ttatccgcat taatgttatg
    22441 cacagagctg taagcgccta acgccgactg cgcatagact acacgatcgc ctgccttaat
    22501 atgctttaca ccactgccga ctttactcac gatgcctgcc gcttcggtac ctaatccgct
    22561 gggtagtgat ggcggcgggt aaaggccgct gcggatatac gtgtcgataa aattgatgcc
    22621 gatggcttta ttttcgacct ggatttcatt ctctgccgga tcggcaggag tgaactctac
    22681 ggcttgaagt acttccgggc caccgtgctt gtgaaattca attcgtgttg ccatgtgtcc
    22741 tccagaacgt atcgtcaggg tctgcttcat atggtaaagt ttcgacccat tctttatctc
    22801 ggtaactcca ttcactatgg caggaaataa acccttcaac aaacagcagg ctgaaccccg
    22861 cgaacgcgat ccacaagttg ccgggctgaa agtgcctccg cactcgatcg aagcggagca
    22921 gtcggtgttg ggcggtttaa tgctggataa cgaacgctgg gacgatgtag ccgagcgtgt
    22981 ggtggcagac gatttttaca cccgcccaca ccgccatatc tttactgaaa tggcgcgttt
    23041 gcaggaaagc ggtagtccta tcgatctgat tacccttgcg gaatcgctgg aacgccaggg
    23101 gcaactcgat agcgtcggtg gttttgctta tctggcagag ctgtcaaaaa atacgccaag
    23161 tgcggcgaac atcagtgcct atgcggacat cgtgcgtgaa cgtgccgttg tccgtgagat
    23221 gatttcggtt gcgaatgaga ttgctgaagc cggttttgat ccgcaggggc gcaccagcga
    23281 agatctgctg gaccttgctg aatcccgcgt ctttaaaatt gctgaaagtc gtgcaaacaa
    23341 agacgaaggg ccgaagaaca tcgccgatgt gctcgacgca accgtggcgc gtattgagca
    23401 gttgtttcag cagccacacg atggcgttac cggagtaaac accggttatg acgatctcaa
    23461 caaaaaaacc gctggcttgc agccgtcgga tttgatcatc gtcgccgcgc gtccgtcgat
    23521 gggtaaaaca acatttgcga tgaacctcgt cgaaaacgcg gcgatgttgc aggataaacc
    23581 agtacttatc ttctcgctgg agatgccttc agaacagatt atgatgcgtt ctctggcgtc
    23641 gctgtcgcgc gttgaccaga ctaaaatccg taccgggcag ctcgatgatg aagactgggc
    23701 gcgcatttcc ggcaccatgg gtattttgct cgaaaaacgc aatatctata tcgatgactc
    23761 ctccggcctg acgccaacgg aagtgcgttc ccgcgcacgc cgtattgccc gtgaacacgg
    23821 cggcatcggg cttatcatga tcgactacct gcaactgatg cgcgtaccgg cgctttccga
    23881 taaccgtacg ctggaaattg cagaaatctc tcgctcgctg aaagcactgg cgaaagaact
    23941 gaacgtgccg gtggtggcgc tgtcccagtt gaaccgttct ctggaacaac gtgccgacaa
    24001 acgcccggtc aactccgacc tgcgtgaatc tggctctatc gagcaggatg cggacttgat
    24061 catgtttatc tatcgtgatg aggtgtatca cgaaaacagt gatttaaaag gcatcgcgga
    24121 aattattatc ggtaaacaac gtaacggccc aatcgggacg gtacgcctga cctttaacgg
    24181 tcaatggtcg cgcttcgaca actatgcggg gccgcagtac gacgacgaat aataattatt
    24241 ttatgaatta ggtaattaaa gcaaacactt atcaaggaac acaaatgcaa gcggcaactg
    24301 ttgtgattaa ccgccgcgct ctgcgacaca acctgcaacg tcttcgtgaa ctggcccctg
    24361 ccagtaaaat ggttgcggtg gtgaaagcga acgcttatgg tcacggtctt cttgagaccg
    24421 cgcgaacgct ccccgatgct gacgcctttg gcgtagcccg tctcgaagaa gctctgcgac
    24481 tgcgtgcggg gggaatcacc aaacctgtac tgttactcga aggctttttt gatgccagag
    24541 atctgccgac gatttctgcg caacattttc ataccgccgt gcataacgaa gaacagctgg
    24601 ctgcgctgga agaggctagc ctggacgagc cggttaccgt ctggatgaaa ctcgataccg
    24661 gtatgcaccg tctgggcgta aggccggaac aggctgaggc gttttatcat cgcctgaccc
    24721 agtgcaaaaa cgttcgtcag ccggtgaata tcgtcagcca ttttgcgcgc gcggatgaac
    24781 caaaatgtgg cgcaaccgag aaacaactcg ctatctttaa taccttttgc gaaggcaaac
    24841 ctggtcaacg ttccattgcc gcatcgggtg gcattctgct gtggccacag tcgcattttg
    24901 actgggtgcg cccgggcatc attctttatg gcgtctcgcc gctggaagat cgctccaccg
    24961 gtgccgattt tggctgtcag ccagtgatgt cactaacctc cagcctgatt gccgtgcgtg
    25021 agcataaagc cggagaacct gttggttatg gtggaacctg ggtaagcgaa cgtgataccc
    25081 gtcttggcgt agtcgcgatg ggctatggcg atggttatcc gcgcgccgcg ccgtccggta
    25141 cgccagtgct ggtgaacggt cgcgaagtac cgattgtcgg gcgcgtggcg atggatatga
    25201 tctgcgtaga cttaggtcca caagcgcagg acaaagccgg ggatccggtc attttatggg
    25261 gcgaaggttt gcccgtagaa cgtatcgctg aaatgacgaa agtaagcgct tacgaactta
    25321 ttacgcgcct gacttcaagg gtcgcgatga aatacgtgga ttaatcgttc tgtaatattt
    25381 gattgtctgt gccggatgcg gcgtgaatgc cttatccggc caataaaatc ctaaaaattc
    25441 aataagttga tgttctttca tgctcttata aaggtcgtgc ctctggcgga tgtacgtttg
    25501 tcatgagtct cactctgttg ctaattgccg ttcgctcctg aacatccact cgatcttcgc
    25561 cttcttccgg tttattgtgt tttaaccacc tgcccgtaaa cctggagaac catcgcgtgt
    25621 ttcaaaaagt tgacgcctac gctggcgacc cgattcttac gcttatggag cgttttaaag
    25681 aagaccctcg cagcgacaaa gtgaatttaa gtatcggtct gtactacaac gaagacggaa
    25741 ttattccaca actgcaagcc gtggcggagg cggaagcgcg cctgaatgcg cagcctcatg
    25801 gcgcttcgct ttatttaccg atggaagggc ttaacagcta tcgccatgcc attgcgccgc
    25861 tgctgtttgg tgcggaccat ccagtactgc aacaacagcg cgtagcaacc attcaaaccc
    25921 ttggcggctc aggggcattg aaagtgggcg cggatttcct gaaacgctac ttcccggaat
    25981 caggcgtctg ggtcagcgat cctacctggg aaaaccacgt agcaatattc gccggggctg
    26041 gattcgaagt gagtacttac ccctggtatg acgaagcgac taacggcgtg cgctttaatg
    26101 acctgttggc gacgctgaaa acattacctg cccgcagtat tgtgttgctg catccatgtt
    26161 gccacaaccc aacgggtgcc gatctcacta atgatcagtg ggatgcggtg attgaaattc
    26221 tcaaagcccg cgagcttatt ccattcctcg atattgccta tcaaggattt ggtgccggta
    26281 tggaagagga tgcctacgct attcgcgcca ttgccagcgc tggattaccc gctctggtga
    26341 gcaattcgtt ctcgaaaatt ttctcccttt acggcgagcg cgtcggcgga ctttctgtta
    26401 tgtgtgaaga tgccgaagcc gctggccgcg tactggggca attgaaagca acagttcgcc
    26461 gcaactactc cagcccgccg aattttggtg cgcaggtggt ggctgcagtg ctgaatgacg
    26521 aggcattgaa agccagctgg ctggcggaag tagaagagat gcgtactcgc attctggcaa
    26581 tgcgtcagga actggtgaag gtattaagca cagagatgcc agaacgcaat ttcgattatc
    26641 tgcttaatca gcgcggcatg ttcagttata ccggtttaag tgccgctcag gttgaccgac
    26701 tacgtgaaga atttggtgtc tatctcatcg ccagcggtcg catgtgtgtc gccgggttaa
    26761 atacggcaaa tgtacaacgt gtggcaaagg cgtttgctgc ggtgatgtaa tgcaggaaag
    26821 caggctggag ctacccagcc tgcagtgaaa ttaaactgtc gtcgctttca ctctttcttt
    26881 atagatgatt tttttgatgc catcgttcta cgtgagagat aataaacgtt attagttctt
    26941 ttattgttaa gcttatccta attatctgga attccttatc ctgttttttt gggtggagta
    27001 ataatttctg cacatctaac tattattaaa tttaataata agtatcgggt ttgttacgat
    27061 agaatttttc tatatcctca agaatatgct ttgccttatt gcctgtgact ttctgagatt
    27121 caattatagt atcgttaaat tctaaagtta aagagagctc tttttttccg ctttgtgtaa
    27181 gtttaacctt atattgatta ccaatatcaa ataaaaaggt cctgtaatga gaataatcac
    27241 ctttaacctc ctcggcaaaa cagcaaagcg tgcaaatata cataaagtct ttcttacccc
    27301 tgattttgtt aactaaatca ataaatgcgt gaaaaacttt acttgcaata gaacttgata
    27361 cttcttctcc aggtctacaa aaatcttttg caatttttaa ctgtgtttct atgtataaaa
    27421 agaaatcttt agcataagaa aaatctttgg cataagagat atctttatta tttagtgcat
    27481 ctagctcaac aggtaaagat gtcgttttgt tgctattcac atatgatata ttcatatcat
    27541 catctccatc gggagtaata aaattagtca ccattgtagg atagggttct ggtcaatcag
    27601 gaatcatctt tataaacttc gattgttttt gtaatgctgt atcattaagt tcattaaatc
    27661 gtacagcaga taaatgttct atcaaatttc gctcatttgc agaggattca tcataataaa
    27721 cgtaaaatta atgtatcctt acatcgagta ataaacattt tttatacaaa aaaagacagg
    27781 aacgtattta ctgggttaaa cataatcatc ctgcttttca tcacaaaaac cgcagataat
    27841 ccttcctttc cccggcagct ggcgttatgg tcagatggtt tttgcaacaa atctcacaat
    27901 aaaaagtttc aacatactga ctatttaggg aaaaatatgc gcaagatcac acaggcaatc
    27961 agtgccgttt gcttattgtt cgctctaaac agttccgctg ttgccctggc ctcatctcct
    28021 tcaccgctta accctgggac taacgttgcc aggcttgctg aacaggcacc aattcattgg
    28081 gtttcggtcg cacaaattga aaatagcctc gcagggcgtc cgccaatggc ggtggggttt
    28141 gatatcgatg acacggtact tttttccagc ccgggcttct ggcgcggcaa aaaaaccttc
    28201 tcaccagaaa gcgaagatta tctgaaaaat cctgtgttct gggaaaaaat gaacaatggc
    28261 tgggatgaat tcagcattcc aaaagaggtc gctcgccagc tgattgatat gcatgtacgc
    28321 cgcggtgacg cgatcttctt tgtgactggt cgtagcccga cgaaaacaga aacggtttca
    28381 aaaacgctgg cggataattt tcatattcct gtcaccaaca tgaatccggt gatctttgcg
    28441 ggcgataaac cagggcaaaa tacaaaatcg caatggctgc aggataaaaa tatccgaatt
    28501 ttttatggcg attccgataa cgatattacc gctgcacgcg atgtcggcgc tcgtggtatc
    28561 cgcattctgc gcgcctccaa ctctacctac aaacccttgc cacaagcggg tgcgtttggt
    28621 gaagaggtga tcgtcaattc agaatactga cagagcggga gagcgtgaca ctctcccgaa
    28681 tgctgttttt ttaatcacac ctttatcctt tcgctgtctt gctgcaaact gattaagaga
    28741 gttttatcaa ggagcagcac atgtggtatc aaaagacgct cacgcttagc gccaaatctc
    28801 gtgggtttca tctggtaacg gatgaaattc tgaatcagct ggctgatatg ccgcgcgtta
    28861 acatcggctt actgcatctg ttgctgcaac atacctccgc ctctctgaca cttaatgaga
    28921 actgcgatcc caccgtacgc cacgacatgg agcgtttttt cctccgcacc gttcccgaca
    28981 acggaaatta tgagcatgac tatgagggag cagacgatat gccttctcat atcaaatcct
    29041 caatgctggg aacatcgctt gtattgccgt tacataaagg gcgtattcag accggcacct
    29101 ggcaaggcat ctggctgggg gaacatcgca tccacggcgg atcgcgtcgc atcatcgcga
    29161 cactacaagg ggagtaaaaa atgaccattt cggagttgct acaatattgc atggcaaaac
    29221 caggcgcaga acagagcgtg cataacgact ggaaagcgac gcagatcaaa gtggaagatg
    29281 tactgtttgc gatggtgaaa gaagtagaaa atcgcccagc tgtttcgctg aaaaccagcc
    29341 cggagctggc ggagctgcta cgtcagcagc acagcgatgt gcgtcctagc cgccatctga
    29401 ataaagcgca ctggagcacc gtgtatctcg acggttcgct gccggattcg caaatctatt
    29461 atctggtgga tgcgtcttat cagcaggcag tgaatttact accggaagaa aagcgtaaat
    29521 tgctggtaca actctgaaag gaaaaggccg ctcagaaagc ggccttaacg attacagcat
    29581 cggcttgagg aagcgtgccg tatgcgaagc ttcgcactcc gcgacggttt ctggcgtacc
    29641 ggagacgagg atttcgccgc cgccactgcc gccttctggt cccaggtcga caatccagtc
    29701 agcggttttg atcacgtcga gattgtgctc aatcaccaca atggtattgc cctgatcgcg
    29761 cagtttatgc agcacgtcga ggagttgctg aatatcggcg aagtgcagac cggtggtcgg
    29821 ctcgtcgaga atatacagcg tctgcccggt gccgcgtttt gacagctcac gcgccagctt
    29881 cacgcgctgg gcttcaccac cagaaagtgt ggttgcggac tgccccaggc gaatgtacgt
    29941 cagaccaacg tccatcaacg tttgcagctt acgcgccaga gctggcaccg catcaaagaa
    30001 ctcacgcgcc tcttcgatgg tcatatccag cacttcgtgg atggttttgc ctttgtactt
    30061 aatttccagc gtttcacggt tatagcgttt acctttgcac tgatcgcacg gcacgtagat
    30121 atccggcagg aagtgcatct ccactttgat cacaccgtcg ccctgacagg cttcgcagcg
    30181 cccgccacgg acgttaaagc tgaaacgtcc tggcgtataa ccacgcgcac gggattccgg
    30241 tacgcccgca aacagttcgc gcacaggtgt aaacacgccg gtataggtcg ccgggttaga
    30301 acgtggagta cgaccaattg ggctttggtc gatatcgatc actttatcga aatgctccag
    30361 cccctgaata tcgcgatacg gtgccggttc ggcgatggtc gcaccattca actggcgttg
    30421 ggcaatcggg aacagtgtgt cgttaatcag cgtcgattta ccggaacctg aaacccctgt
    30481 gatgcaggta aacagaccga ctggcagcgt gagcgtcacg tccttcaggt tgttgccgcg
    30541 tgcacctgtc agcttcagca ctttttctgg attcgccgga acgcgtttct tcggcacttc
    30601 aatcttgcgt ttaccgctca tgtactgccc ggtcaacgat tcaggcaccg ccataatcgc
    30661 ttccagcgga ccttctgcga ccacttcacc gccgtgaaca cccgcaccag gaccgatatc
    30721 gatcacatgg tcagcggcgc gaattgcgtc ttcgtcatgc tccaccacaa tcacggtatt
    30781 accgagatcg cgcagatgga taagcgtacc caacaggcgc tcgttatcgc gctggtgcag
    30841 gccgatagac ggctcgtcca gtacatacat aacgccaacc aggcccgcac caatctggct
    30901 cgccagacgg atacgctggg cttcaccgcc ggaaagtgtt tctgccgagc gggaaagtgt
    30961 caggtaattc aggccgacgt ttacgaggaa tttcaggcga tcgccgatct ctttaagaat
    31021 tttttccgca atcttcgccc gctgaccagc tagtttgaga ttgttgaaga attccatcgc
    31081 atgaccgatg ctcatgtcgg agatagcagg cagcggcgta ttctcgacat aaacgtggcg
    31141 cgcttcccga cgcagacgcg tcccttcgca gctggcgcat gggcgattgc tgataaactt
    31201 ggctaattct tcacgtaccg cactggattc tgtctcttta taacggcgct ccatattgtg
    31261 cagcacgcct tcgaacggat gacgacggat ggaggtatcg ccacgatcgt tcatgtattt
    31321 gaattcaatg ttttctttgc cagaaccgta caacaccact ttatgcacgt tcgcgctcag
    31381 gctgccccac ggcgcttcga cgtcgaactt atagtgatct gccagcgatt tcagcatctg
    31441 gaagtaatag aagttgcggc gatcccagcc acggatcgca ccgccagcca gtgacagctc
    31501 ggggttttgg atcacgcggt caggatcgaa atattgctgt acgccaaggc cgtcacaggt
    31561 tgggcaggca cctgccgggt tgttaaacga aaacagtcgc ggctccagtt cacgcatact
    31621 gtagccgcaa attgggcagg cgaagttggc ggagaacagc agctcttccg ctttcgggtc
    31681 gtccatatcc gccactaccg ctgtaccacc ggaaagctcc agcgcggttt caaacgactc
    31741 cgcaagacgt tgggtaagat cgtcacgcac cttgaagcga tcaaccacca cttcaatggt
    31801 atgtttcttt tgcagttcca gtttcggcgg atcggaaaga tcgcagactt cgccatcaat
    31861 acgagcacgg atgtaaccct ggcttgccag gttctccagc gttttggtgt gttcgccttt
    31921 gcgctcttta atgattggcg cgagcagcat cagacgcttg ccttccggct gcgacagcac
    31981 gttatccacc atctggctga cggtttgcgc cgccagcggg acgtcgtggt ccggacagcg
    32041 cggttcgccg acgcgggcga acaacaaacg caaatagtcg tggatttcgg tgattgtccc
    32101 caccgtagaa cgcgggttat gagacgtcga tttctgctca attgagatgg caggagaaag
    32161 cccctcaata tgatcgacgt ccggcttttc catcagtgac agaaactgcc gcgcgtaggc
    32221 ggaaagggat tcaacgtaac ggcgctgccc ttcggcatat aaggtgtcga aagcgagcga
    32281 ggatttgcca gaacccgaaa gcccggtcac gacaatgagc ttgtcgcggg ggataacgag
    32341 gttgatgttt ttgagattat gggtgcgggc gccccgaact tcgatcttat ccattcacct
    32401 ttcccggatt aaacgctttt ttgcccggtg gcatggtgct accggcgatc acaaacggtt
    32461 aattatgaca caaattgacc tgaatgaata tacagtattg gaatgcatta cccggagtgt
    32521 tgtgtaacaa tgtctggcca ggtttgtttc ccggaaccga ggtcacaaca tagtaaaagc
    32581 gctattggta atggtacaat cgcgcgttta cacttattca gaacgatttt tttcaggaga
    32641 cacgaacatg gccagcagag gcgtaaacaa ggttattctc gttggtaatc tgggtcagga
    32701 cccggaagta cgctacatgc caaatggtgg cgcagttgcc aacattacgc tggctacttc
    32761 cgaatcctgg cgtgataaag cgaccggcga gatgaaagaa cagactgaat ggcaccgcgt
    32821 tgtgctgttc ggcaaactgg cagaagtggc cagcgaatat ctgcgtaaag gttctcaggt
    32881 ttatatcgaa ggtcagctgc gtacccgtaa atggaccgat caatccggtc aggatcgcta
    32941 caccacagaa gtcgtggtga acgttggcgg caccatgcag atgctgggtg gtcgtcaggg
    33001 tggtggcgct ccggcaggtg gtaatatcgg tggtggtcag ccgcagggcg gttggggtca
    33061 gcctcagcag ccgcagggtg gcaatcagtt cagcggcggc gcgcagtctc gcccgcagca
    33121 gtccgctccg gcagcgccgt ctaacgagcc gccgatggac tttgatgatg acattccgtt
    33181 ctgatttgtc attaaaacaa taggttatat tgttttaagg tggatgatta aagcatctgc
    33241 cagccataaa aaagaagcct ccgttatgga ggcttctacg tatcaggtca aaatcattgg
    33301 ccattgtggc ggtgtctgat tcatctcttc tatcatcacc gactcgatgt tgtgccagat
    33361 agcggtgatg tccgccattg tgatgccaag caaccccatt gcgaaccaac aggcggaaac
    33421 aacgcccaga ccgctgctga tcaccgcaag cccgacataa tcagaccgac gaaaacggat
    33481 gttgagtgtg ctggccagaa acatcattac ggcactaaga agttgccagc gaagaagaac
    33541 cacgccagtg gtgagggtag ccatcaaaca attcctctga aaagagccga tgccctggac
    33601 agcgcggcgt tgttcacggg aggcgtggta cactctggct atcgcggggc ttgcagaaca
    33661 caaaaatgaa acactctgtt tgtttcatta attttgtgaa ctatatcaca attgattgtt
    33721 tgttagccat attaggccgt gacttttatt gctgtacaga ttatgtggtt tttcagtggc
    33781 attaagggca tatcttcccg ccgcctctgc attcctgtag gaaattaatt ttgaatatca
    33841 atgaattatt ttcatccagg tgacgattag aaaggtatca atttcaaatc aggcaaaagt
    33901 gctatttata ccgtaagatt tatctaaaga cgtcggtacc cagggttttc accttgcaat
    33961 ggccgggtat aaacaggcag gaaattgata gcaatgagtc atcgtgcacg acaccaatta
    34021 ctggcgttgc cgggcattat ctttttagtt ctctttccca tcattctttc gctatggatt
    34081 gccttccttt gggcaaaatc agaagtgaat aatcagctcc gaacctttgc tcaactggca
    34141 ctggataaat ccgagctggt cattcgccag gcagatttag tgagcgatgc agctgaacgc
    34201 tatcaggggc aagtttgcac tccagcccat caaaagcgaa tgttgaatat tattcgtggc
    34261 tatctttata ttaatgaatt gatctatgcc cgtgataacc attttttatg ctcatcgctg
    34321 atagcgcctg taaacggcta tacgattgca ccggccgatt ataagcgtga acctaacgtt
    34381 tctatctatt attaccgcga tacgcctttt ttctctggct ataaaatgac ctatatgcag
    34441 cggggaaatt atgtggcggt tatcaaccct ctcttctgga gtgaagtgat gtctgatgac
    34501 ccgacattgc aatggggtgt gtatgatacg gtgacgaaaa cctttttctc gttaagcaaa
    34561 gaggcctcgg cagcaacgtt ttctccgctg attcatttga aggatttaac cgtacaaaga
    34621 aatggctatt tatatgcgac agtttattcg acaaaacgcc caattgcagc cattgttgcg
    34681 acttcatatc aacgtcttat aacccatttt tataatcatc ttatttttgc gttgcccgcc
    34741 ggtattttgg ggagtcttgt tctgctatta ctctggctac gtattcgaca aaactattta
    34801 tctcccaaac gtaaattgca acgcgccctc gaaaaacatc aactttgtct ttattaccag
    34861 ccaataatcg atatcaaaac agaaaaatgt atcggcgctg aagcgttgtt acgttggctt
    34921 ggtgagcagg ggcaaataat gaatccggca gagtttattc cgctggcaga aaaggagggg
    34981 atgatagaac agataactga ttatgttatt gataatgtct tcctcgatct gggcgcgtac
    35041 ttggcaacac atgccgatcg ctatgtttct attaacctgt cggcctccga ttttcatacg
    35101 tcacggttga tagcgcgaat caatcagaaa acagagcaat acgcggtgcg tccgcagcaa
    35161 attaaatttg aagtgactga gcatgcattt cttgatgtcg aaaaaatgac gccgattatt
    35221 ctggctttcc gccaggctgg ttacgaagtg gcaattgatg attttggtat tggctactct
    35281 aacttgcata accttaaatc attgaatgtc gatattttga aaatcgataa atcgtttgtt
    35341 gaaacgctga ccacccacaa aaccagtcat ttgattgcgg aacacatcat cgagctggcg
    35401 cacagcctgg ggttaaaaac gatcgctgaa ggcgtcgaaa ctgaggagca ggttaactgg
    35461 ctgcgcaaac gcggcgtgcg ctattgccag ggatggtttt ttgcgaaggc gatgccgccg
    35521 caggtgttta tgcaatggat ggagcaatta cccgcgcggg agttaacgcg cgggcaataa
    35581 aactacaggc ggtggcgata atcgctggga gtgcgatcaa actgccgacg gaaaacgcgg
    35641 gagaaggtct gctgcgagac ataacccagg tccattgcga tatcaaaaat cggacgctcg
    35701 gtggtgcgca actcaacggc ggccagtaac aggcgacgtt ggcgaatgta atcgccaagc
    35761 gtctgatgcg tcaccgtgcg gaacattcgt tgcaagtacc actttgaata gcctgatttt
    35821 tttgcgacta catcaatgtt aagcggctgg tcaatatgct cgtcaatcca tgcgataaga
    35881 tcctgaataa ttttctgatg ggacataaat ctgcctcttt tcagtgttca gttcgttaat
    35941 tcatctgttg gggagtataa ttcctcaagt taacttgagg taaagcgatt tatggaaaag
    36001 aaattacccc gcattaaagc gctgctaacc cccggcgaag tggcgaaacg cagcggtgtg
    36061 gcggtatcgg cgctgcattt ctatgaaagt aaagggttga ttaccagtat ccgtaacagc
    36121 ggcaatcagc ggcgatataa acgtgatgtg ttgcgatatg ttgcaattat caaaattgct
    36181 cagcgtattg gcattccgct ggcgaccatt ggtgaagcgt ttggcgtgtt gcccgaaggg
    36241 catacgttaa gtgcgaaaga gtggaaacag ctttcgtccc aatggcgaga agagttggat
    36301 cggcgcattc ataccttagt agcgctgcgt gacgaactgg acggatgtat tggttgtggc
    36361 tgcctttcgc gcagtgattg cccgttgcgt aacccgggcg accgcttagg agaagaaggt
    36421 accggcgcac gcttgctgga agatgaacaa aactaaagcg ccacaagggc gctttagttt
    36481 gttttccggt ctttgtcttt ctctctatcc cgctggtaca caggagggtt tcccccgacg
    36541 tcaacacacc ccattcgagc acgtggtgga ggttccggtt ggtgttgatg ctttaattgt
    36601 atgtcaccgg catttcttcg ccagtgtaaa agtatacttt ttatccgcaa tatttttgtc
    36661 atctcagacg attttttatc gcaatcctga acggtatacg gctcgataac gctgcaatct
    36721 tgcgcaccga cgataacgtt tgcgcatcaa ttgcctggtt tttcatcgtc aagacaataa
    36781 aagagaaaaa agcagcaaac ttcggttgaa aaagccgcta tgatcgccgg ataatcgttt
    36841 gcttttttta ccacccgttt tgtatgcgcg gagctaaacg tttgcttttt tgcgacgcag
    36901 taaattgtcg caaacctgga gcaggaagat aacgtttcgc tggcagggga ttgtccgcca
    36961 cgcatcttga cgaaaattaa actctcaggg gatgttttct tatgtctacg ccatcagcgc
    37021 gtaccggcgg ttcactcgac gcctggttta aaatttcaca acgtggaagc actgtccgtc
    37081 aggaagtggt tgccgggtta acaacgtttc tggcgatggt ctactcggtc atcgtcgttc
    37141 caggtatgtt gggtaaagca ggcttcccgc ctgcggcagt tttcgttgca acctgtctgg
    37201 ttgccggact cggttctatc gtgatgggtc tgtgggctaa tctgccgttg gcgattggtt
    37261 gcgccatctc cctgacggcg tttaccgcat tcagcctggt gctggggcaa catattagcg
    37321 tacctgtcgc gctgggtgcc gtgttcctga tgggggtgct gtttacggta atttctgcca
    37381 cgggtatccg tagctggatt ttgcgcaact tgccgcacgg tgtggcgcac ggcacgggga
    37441 tcggtatcgg tctgttcctg ctgctcattg ccgctaatgg tgtcggtctg gtaattaaaa
    37501 acccgcttga tggtctgccc gttgcgctgg gtgatttcgc gaccttcccg gtgattatgt
    37561 cactggtagg tctggcggtg atcatcggcc tggaaaaact gaaagtccct ggtggcattc
    37621 tgctgaccat tatcggtatc tcaattgtcg gtttgatctt cgatcctaac gtccatttct
    37681 ccggcgtttt cgccatgcct tcattgagcg atgaaaacgg caattcactg attggcagcc
    37741 tggacattat gggcgcgctg aatcctgtag tcctgccaag cgttctggcg ctggtaatga
    37801 cggcagtatt tgatgccacc ggaactatcc gtgccgtcgc cggccaggcg aacctgctgg
    37861 ataaagatgg gcaaattatc gacggtggga aagcactgac cactgactcc atgagcagcg
    37921 ttttctctgg cctggtgggt gcagctccgg cagcggtata catcgagtct gcggcgggta
    37981 cggcggcggg cggtaaaacc ggtttgacgg ctatcaccgt tggcgtgctg ttcctgctga
    38041 ttctgttcct ctctccgctc tcttacctcg ttccggggta tgcaacggct ccggcgctga
    38101 tgtacgttgg cctgctgatg ctgagcaacg tggcgaaaat cgactttgct gattttgttg
    38161 atgcgatggc gggtctggtt acggcggtat tcatcgtgct gacctgtaac atcgtaacag
    38221 gcatcatgat cggcttcgcg actctggtga ttggtcgtct ggtttccggc gaatggcgca
    38281 agttgaacat cggtacggtc gttatcgccg tggcgctggt gaccttctat gcgggtggct
    38341 gggctatcta atctttcatc ggattctgaa aacgggtggc aatggctgcc cgtttttatt
    38401 ttctccgcac attgtgtgag cttttgcgat attctgaaaa aatgagaatt caggcataac
    38461 gtctttctca gatcataaaa aacatcgcaa acagggaacg catggaaatc ttcttcacca
    38521 tactgataat gaccctcgtg gtctcgctgt ccggggtggt cactcgtgtc atgccctttc
    38581 agatcccgct tccgcttatg caaatcgcca tcggtgcgct actggcgtgg ccgacgtttg
    38641 gtttgcatgt ggagtttgat cctgaactct ttttagtctt gtttatcccg ccgttgctgt
    38701 tcgctgatgg ctggaaaacg ccgacccgtg aatttctcga acatggtcga gagattttcg
    38761 gcctcgcgct ggcgctggtg gtggtcaccg tggtcggcat tggcttcctt atttactggg
    38821 tggtgccggg cattccgctg atccccgcct ttgcgctggc ggcggtactt tctccgaccg
    38881 atgctgtggc gctctccggg attgttggcg aagggcgcat cccgaaaaaa atcatgggca
    38941 ttttgcaggg cgaagcgttg atgaatgacg cttccggcct ggtgtcgttg aagtttgccg
    39001 tggcagtggc gatggggacg atgatcttca ccgtcggcgg tgcgacggtc gaatttatga
    39061 aagtagccat tggtggtatt cttgccggtt ttgtggtgag ctggctgtat ggtcgctcgc
    39121 tgcgattcct cagccgctgg ggcggtgatg aacccgcgac gcagatcgtc ctgctgttcc
    39181 tgctgccatt cgcttcttat ttgattgccg aacatattgg cgtttccggc atcctcgctg
    39241 cggttgccgc cgggatgacc atcacccgct ccggtgtgat gcgccgtgcg ccactggcaa
    39301 tgcgcctgcg cgcaaacagc acctgggcga tgctggaatt tgtctttaac ggcatggtgt
    39361 tcctgctgtt aggtctgcag ctgccaggta ttctggagac gtcgctgatg gcggcagaaa
    39421 tcgaccctaa cgtcgaaatc tggatgctgt ttaccgatat tattctgata tatgcggcgc
    39481 tgatgctggt ccgtttcggc tggctgtgga cgatgaaaaa gttcagcaac cgcttcctga
    39541 agaagaagcc gatggagttt ggttcgtgga ccacacgaga aatcctgatc gcgtctttcg
    39601 ccggggtgcg tggggcgatc actctggccg gtgtgctctc tatcccgctg ctcttgccgg
    39661 atggtaacgt cttcccggcg cgctatgagc tggtgttcct ggcggctggc gtcattctct
    39721 tctcgctgtt tgtcggcgtg gtgatgttgc ctattctgct acaacacatt gaagtcgcgg
    39781 accattcgca acaattgaaa gaagaacgta ttgcgcgagc ggcaacggca gaagtggcga
    39841 ttgtggcgat ccagaaaatg gaggagcgtc tggcggcgga taccgaagag aatatcgata
    39901 accagctgct tacggaggtc agttctcgcg tcattggtaa cctgcgtcgt cgcgccgatg
    39961 gacgtaacga cgttgaaagt tccgtgcagg aagagaacct tgagcgtcgc ttccgtctgg
    40021 cggcattgcg ttctgaacgt gctgaactgt accacctgcg cgccacgcgg gagatcagca
    40081 acgaaacgct gcaaaaatta ctgcacgatc tcgatttgct tgaagcgttg ctaattgagg
    40141 aaaatcagta agccgaaagc gacgtaacca ggcccgaggt aatctgaaaa aggtgcaatc
    40201 ttttgcacct ttaacaggtt agctcgggct aacattcatg attctaaaac aaataagtta
    40261 tctctcttgt aaagtcaatt cattggctgt tcttcgattt aacctcaatc tgcgtgttgc
    40321 ataatatctg cagtaatctt catagcgact gcattaatat tatcctcagt ttgtgacatg
    40381 ccagtatgag aggtacaatc ttcttcaaag aatttctgtc gtgttaccag ggtatcacta
    40441 tcaatgggga tattttcctc cccacggagt atggaaatgc ccattccctg gacgctagcc
    40501 tcatcaaaat taacccgata gagacgggta ttaaacatac aggcttttgt taaaatagaa
    40561 cagttgaaat ggcagttgct taaattggca ttattaaaat taacattagc catattagag
    40621 tcggtacata taaccatttc gaggaatgta tttgttagcc acgtacaaaa gagtttgcta
    40681 tcaattaagt ttatattctg gaggattgct ttgttcagat tgacgcgaga cagatcagca
    40741 tgttcaaagt taattaaatc gagtttagag aaactaaggt cggctttaaa taaattaaca
    40801 tatggcgcta ttactttata aagatttacc tcagccatgt acgctatata aaaatttgaa
    40861 tagtcaagaa aggtattttt aatattggct ctattcattg ctgcattgta gaattgaaca
    40921 tgcatagcgg agcatgcatt tagagttccg tcggccattt ttatctgagc gaaatttgag
    40981 tcatcgagaa tacaattatc aaaaagtatt ttttccatta tggcgtaata aaaattcgtt
    41041 ttatacatat tacagtcctg gaaaatagcg ttttgtagtc tgcaacctgc aaaattcgaa
    41101 ttggataaat cttcatcttt aatcacgata cctttcaggc taaggtgtga aaaatcgtaa
    41161 ccaccattta ctgtctgtgg aatttcgata gtattattat ggacggcgac attatttata
    41221 aaatatacca ccttaatgga tacttccttt tctttactaa ggtaaactct tctcgtaatt
    41281 ctatcgtcac atttattatt tgcatataaa gctatatcta taaaatgttt atgtattttc
    41341 ctgtccgttt ccttcgtgat ttttcctgat ggtaaactat cataaattct tttcattttc
    41401 tgaatcaaac ctatcacttt ttcatgatca tccatcttga aaagagagag ggctttattg
    41461 tcaattaacg tgtttttatg atgtttgctg taattatatg attttgtttt ctttgtatgt
    41521 gatgaacttg tgaaagagtg gttatcgtta atctggcaaa gagagaaaaa catattattt
    41581 aaaccattat agcgcataaa atatccttat aattaacaag aaagaaaagg catattctct
    41641 gcattatcat tttctacgac tgtcaaaaat cgctcatttt ttaatgagtt tatttgttta
    41701 atattatggg aaaaggtgat gcatgctgga gaggaagagt attccccggt caggcgaccg
    41761 gggaagggga gagattaatg tgcgcggcct tgctcaacgc caaagccggt ctgggagcgg
    41821 ataaactgtg cacggaacag ttcacgctcg cgcgcacctt ccgctgagtt atcggttgcc
    41881 gagaagaacc agatgccgag gaatgccacg ctgatagaga acagcgccgg gtattcatac
    41941 gggaagatgg ctttttcgtg accaaggatc tgtacccaaa tcgtcgggcc gaggatcatc
    42001 agtaccactg cggtaatcag ccccagccag ccacccatca tcgcgccacg agtggtcagt
    42061 ttcgaccagt acatagaaag cagaatgatc gggaagttac agctcgctgc gatggcaaac
    42121 gccaggccta ccatgaaggc gatgttctga ttctcaaaca gcacgccgag gataatggca
    42181 atcacaccta aaatcagcac ggtgattttc gatacccgca gttcttcacg ttcggtcgcg
    42241 ccttttttga agacgttagc gtacaggtca tgcgaaaccg ctgatgcgcc cgccagcgtc
    42301 agaccagcaa ccaccgcaag aatggtggca aaagcaaccg ctgaaataaa accgaggaac
    42361 aggttgccgc ccactgcatt cgccaggtga acggccgcca tgttgttacc accaatcaga
    42421 tggcccgccg cgtctttata ttccggattc gcaccaacca gcatgatcgc gccgaagccg
    42481 ataataaagg tcagaatata gaagtagccc ataaatccgg tagcgtagaa cacgctctta
    42541 cgcgcttcgc gggcatcgct gaccgtaaag aagcgcatca gaatgtgcgg caagccagcc
    42601 gtaccaaaca tcagtcccag acccagagag agcgcggaga tcggatcttt caccagtccg
    42661 cccggtttca tgatgtcgac acctttcggg tgtaccgcca tcgcttcgct gaacagattg
    42721 ttgaagctaa agccgacgtg tttcatcacc ataaaggcca taaagctggc accgaacagc
    42781 aacagcacgg ctttgataat ttgtacccag gtggtcgcca gcatgccgcc gaacaggacg
    42841 tacatcatca tcagcacgcc gaccagcacc accgcaatgt gatagttaag gccaaacagc
    42901 agctcgatca gtttacctgc acccaccatc tgggcgataa ggtaaagcgc caccaccacc
    42961 agagaaccac aggccgaaag aatacgaatc ggcccttgtt tcagacggta agaggccaca
    43021 tcggcaaagg tgtagcgccc caggttacgc agacgttcgg caatcaggaa cagaatgatc
    43081 ggccagccca ccaggaagcc cagcgagtaa attaagccgt catagccgga ggtaaacacc
    43141 agcgcggaga tccccaaaaa tgaggcggcg gacatatagt caccggcaat cgccagcccg
    43201 ttctggaagc cagtgatatt gccgcctgcg gtgtagtagt cgctacgaga acgtacgcgt
    43261 tttgatgccc agtaggtaat gccgagcgta aacacgacga aaatcaggaa cataataatc
    43321 gcctgccagt tcgttggctg gcgctctacg gccccgctaa tagcatccgc ggcgttagct
    43381 gcgaaaggga gtgtggcggc aagcgccgtc agaactcttt tcatgatgct tgtacctcat
    43441 gcaggacttc gttattaaga cggtcgaatt cgccgttcgc ccgccagatg tagataccgg
    43501 tgagaacaaa ggagatcaca atcactccaa caccaatcgg aataccgcgt gtgacgctgg
    43561 tgttcggatt cagcggcgtg cccagccagc cgggcgcgaa ggcgatcagt aaaataaagc
    43621 cgatataaac tgccagcata ataatcgaca ggatggtggc aaaccgttgc cgtttttcga
    43681 ctaactccct gaaatgcgca ttgtcttcta tccgctgata aatagtgcca ttcatcacag
    43741 attctccaga ggtaatgtag ggattgtttt aattcccgct cccttatggg agaaggttaa
    43801 cgctcgggta acccttgccg aatgtaggcc ggataaggcg ttatatgccg catccggcaa
    43861 tcaatgcctg atgcgacgct gtcgcgtctt atcaggccta caaaccgtta ccgattcgca
    43921 tccggcaatt gtgggttacg atggcatcgc gatagcctgc ttctcttcaa gcagcttctc
    43981 gacaacgcca ggatcggcaa gcgtcgaggt atcgcccagg ttgctggtat cgcccgccgc
    44041 aattttgcgc agaatacggc gcataatttt gccggaacgg gttttaggca gggagtcggt
    44101 ccagtgcagc acgtctggcg tcgccagcgg gccaatctct ttacgcaccc agttgcggac
    44161 ttctgcgtac agttctggtg acggttcctc cccgtgatta agcgtgacgt aggcgtagat
    44221 cgcctgacct ttaatattgt gcggaatacc tactacggcg gcttcggcaa tcttcggatg
    44281 cgccaccagc gccgactcaa tctctgccgt ccccagacgg tgaccggaga cgttcagcac
    44341 gtcgtccaca cgcccggtga tccagtaata gccatcttca tcgcgacgcg cgccgtcgcc
    44401 gctgaaatac atatttttga aggtggagaa gtaggtctgt tcaaaacgtt cgtgatcgcc
    44461 aaacagcgta cgcgcctgac ccggccagga gtcggtgatc accaggctgc cttcggtagc
    44521 gccttccagc gggttacctt cgttatcgac cagcgccggt tgcacgccga agaacggacg
    44581 tgttgccgaa ccggctttca gctcggtagc gccaggcagc ggcgtgatca tgaaaccgcc
    44641 agtttcggtc tgccaccagg tatcgaccac cggacatttc tcgttgccga tttttttcca
    44701 gtaccactcc cacgcttctg ggttaattgg ctcgcccacg gaaccgagaa tgcgcagcga
    44761 cgaacggtcg gtaccctcga tcgctttatc gccttccgcc atcagcgcgc ggatcgccgt
    44821 gggtgcggta tagagaatat tgacctgatg cttgtccacc acctgcgcca tacgggcagg
    44881 cgtcggccag ttgggtacgc cttcaaacat cagcgtggtc gcaccgcagg ccagcgggcc
    44941 gtacagcaag taactgtgtc cggtcaccca gcccacatcg gcggtgcacc agtagatatc
    45001 acccggatga taatcaaaga catatttaaa ggtcagcgcc gcgtacacca gataaccgcc
    45061 ggtagtatgc agcacacctt ttggcttacc ggtagaaccg gaggtgtaga gaataaacag
    45121 cggatcttcg gcgttcatct cttccgcctg gtgctgatcg ctcgcttgct caaccaggtc
    45181 gtgccaccac aggtcgcgcc cttcctgcca gtcaattttc ccgccagtac gcttcagtac
    45241 caccacatgc tctacgctgg tgacgttcgg gtttttcagc gcgtcatcaa cgtttttctt
    45301 cagcggaata ctgcgcccgg cacgcacacc ttcgtcggaa gtgatcacca gtcgtgagtt
    45361 ggaatcaata atgcgcccgg caacggcttc cggcgagaag ccgccgaaaa tcaccgaatg
    45421 caccgcgcca atgcgggcgc aggccagcat cgcaaccgcg gcttccggca ccatcggcat
    45481 ataaatcgcc accacatcac cttttttaat gcccagctcg agcagggtat tggcgaagcg
    45541 gcagacgtcg cggtgcagct ctttatagct gatatgtttg ctctggctgg cgtcgtcgcc
    45601 ttcccagatg atggcggtgc gatcgccgtt ttcttgcaga tggcggtcaa ggcagtttgc
    45661 cgccagattc agcgtgccgt cctcgtacca tttaatggac acattaccgg gggcaaagga
    45721 ggtatttttc accttctggt acggtttgat ccagtcgaga atttttccct gttcgcccca
    45781 gaaggtatca ggtacgttaa tagattgttg atacatcgcc tcgtactgct gagggtttat
    45841 caggcaacgg tctgcgatgt tggcaggaat ggtgtgtttg tgaatttggc tcatgctttt
    45901 gttctccttg taggatgtta ataatatgtg gcataagcgt taaatgtagg ggtattggca
    45961 gttttgttta gtatttgggc gacagatcac gcaaaagtag aattgtgcaa ataaacggca
    46021 gggtaattat tgaaggtcac caacaaaagt tgattaattc tttgaggaac atgcagttat
    46081 gcatgctgtt gaaaaagagg aagatactga ctaactctaa agtggtattt tacatgcact
    46141 tacaattgat taaagacaac attcacagtg tggttatttg ttacacatag gggcgagcaa
    46201 tgtcatgaca gtgtaggtgc ggttactgtc gtgaaaacaa taaaaaccgc cattgcaaca
    46261 atggcgcaat tcggatgaag cccctatgac aaggataaaa ataaacgcac gccgtatctt
    46321 cagcttattg attccttttt tctttttcac ttctgttcac gctgaacaaa cggcagctcc
    46381 cgcaaaacct gtaactgtgg aagcgaagaa tgaaaccttt gccccgcagc atcccgatca
    46441 atatctctcc tggaaagcca cctcggaaca gtcagagcgt gttgacgccc tggcggaaga
    46501 tccacggctg gtgatcctgt gggcggggta tcccttctcg cgcgattaca acaagccgcg
    46561 tggacatgcc tttgctgtga ccgatgtgcg tgaaaccctg cgtaccggtg cgccgaaaaa
    46621 cgctgaagat ggtccgctac cgatggcgtg ctggagttgt aaaagcccgg atgtggcgcg
    46681 tctgatccag aaagacggcg aagatggata cttccacggt aagtgggcgc gcggcggccc
    46741 ggaaatcgtc aacaacttag gttgtgccga ctgccataac accgcctcac cagagttcgc
    46801 caaaggcaaa ccggagttaa ccctttcccg tccgtatgcg gctcgcgcga tggaagccat
    46861 tggtaaacct tttgagaaag ccggacgttt cgaccagcaa tcgatggttt gcggtcagtg
    46921 ccatgtggag tattacttcg acggcaaaaa caaagcggtt aaattcccgt gggatgacgg
    46981 catgaaagtc gaaaatatgg agcagtatta cgacaaaatt gccttctctg actggactaa
    47041 ctccctgtcg aaaacgccaa tgctgaaagc gcagcacccg gaatatgaaa cctggacagc
    47101 gggcattcac ggtaaaaaca acgtgacctg tatcgactgc catatgccaa aagtgcagaa
    47161 cgccgaaggc aaactctaca ccgaccataa aattggtaat ccgtttgata acttcgccca
    47221 gacttgtgcg aactgccata cccaggacaa agctgccttg caaaaagtgg tcgcggaacg
    47281 taagcagtcg attaacgacc tgaaaatcaa ggttgaagat caactggttc acgctcactt
    47341 cgaagcgaaa gcagcgctgg atgcaggcgc gacggaagcc gaaatgaagc caattcagga
    47401 cgatatccgt catgcccagt ggcgctggga tctggcgatc gcttcccacg gcattcatat
    47461 gcacgcaccg gaagaaggtt tacggatgct cggtacggcg atggataaag cggcggatgc
    47521 acgcaccaaa ctggcgcgcc tgctggcgac caaaggcatc acccatgaaa tccagatccc
    47581 ggatatctca accaaagaga aagcccagca ggccattggc ctgaacatgg aacaaatcaa
    47641 ggccgagaag caggacttca tcaaaacggt gatcccgcag tgggaagaac aggcacgtaa
    47701 aaacggtctg ttaagccaat aaccccgttc cgcctcgcaa ggggcggaaa acacaatgga
    47761 gtgaatatga gcgtattacg ttcgttatta actgccgggg tgctggcgtc gggcctgttg
    47821 tggagcctga acggaattac cgctacccct gcggcgcagg catctgatga tcgctacgaa
    47881 gttacccagc agcgtaaccc ggatgccgcc tgtctggact gtcataagcc agataccgaa
    47941 ggtatgcatg gaaaacatgc ttccgttatc aacccgaata acaaactgcc ggtcacctgc
    48001 accaactgtc acggccagcc atcaccgcaa caccgcgaag gggtgaaaga tgtgatgcgc
    48061 tttaacgagc cgatgtacaa ggttggagag cagaacagcg tctgtatgtc ctgtcatctg
    48121 ccggaacagt tgcaaaaagc gttctggccg cacgatgtcc acgtaaccaa agtggcgtgc
    48181 gccagctgcc attccctgca tccacagcaa gatacgatgc agacgttaag tgacaaagga
    48241 cggattaaga tttgcgtcga ttgccacagc gatcagcgca ccaatccgaa ctttaacccg
    48301 gcgtctgttc cgttgcttaa ggagcagcca tgacctggtc tcgtcgccag tttctcaccg
    48361 gcgtcggcgt gctggcagcc gtcagcggca ccgcagggcg cgtggtggcg aaaacgttga
    48421 atatcaatgg ggtgcgttac ggcatggtgc atgacgagtc gttatgcatc ggctgtaccg
    48481 cctgtatgga tgcttgtcgg gaagtgaaca aggtgccgga aggcgtctcg cgcctgacga
    48541 ttattcgtag cgagccgcag ggcgaatttc ctgatgtgaa atatcgcttc ttccgtaagt
    48601 cttgccagca ctgcgatcat gcgccgtgcg ttgacgtctg cccgaccggt gcgtcttttc
    48661 gtgatgccgc tagcgggatt gtcgatgtta atcctgatct ctgcgtcggt tgtcagtact
    48721 gcatcgccgc ctgtccgtac cgcgtgcgct ttatccatcc ggtcacgaag acggcggaca
    48781 aatgcgattt ctgccgtaaa accaatttgc aggccggtaa gctgcccgcg tgcgttgagg
    48841 cttgcccgac gaaggcgctg acgtttggca atctggacga tcctaacagt gagatttcgc
    48901 aactgctgcg ccagaagcct acttaccgct acaagctggc gctgggaacc aaaccgaagc
    48961 tgtaccgcgt accgtttaaa tacggggagg tgagccaatg acgcagactt ccgcatttca
    49021 ttttgaatcg ctggtgtggg actggccgat tgccatctac ctgtttttga ttggtatttc
    49081 tgccggtctg gtgacgctgg ccgtgctgtt acgtcgcttc tacccgcagg cgggcggtgc
    49141 agacagtacg ttgctgcgca ccacgctgat tgtcgggccg ggcgcggtga tcctcggtct
    49201 gttgatcctc gtcttccacc tgacaagacc gtggaccttc tggaagctga tgttccacta
    49261 cagttttacc tcggtgatgt cgatgggggt gatgctgttt cagctctaca tggtggtgct
    49321 ggtgctgtgg ctggcgaaaa tctttgaaca tgatttgctt gccctgcaac aacgctggtt
    49381 gccgaagctg gggatcgtgc aaaaggttct gagcctgctg acgcccgttc atcgcggact
    49441 ggaaacattg atgctggtgt tggcggtgtt gttgggggct tataccggct ttctgctgtc
    49501 ggcgctgaaa tcgtatccgt tcctcaataa cccgatcctg ccggtgctgt tcctcttctc
    49561 cggcatctcg tccggtgcgg cggtggcgct gatcgccatg gcgatacgcc aacgcagtaa
    49621 cccgcattcc acggaagcgc agtttgtaca ccgtatggaa atccccgtgg tatggggtga
    49681 aatcttcctg ctggtggcgt tttttgtcgg tctggcgctg ggcgatgacg gtaaagtgcg
    49741 tgcgctggtg gcggcattag gtggcggttt ctggacgtgg tggttctggc ttggtgtcgc
    49801 cgggctgggg ctgattgtgc caatgttgct caaaccgtgg gtcaatcgca gttccggcat
    49861 tcctaccgtg ctggcggcgt gtggggccag tctggtcggc gtgttgatgc tgcgcttttt
    49921 cattctctac gccgggcagt taacggtggc gtaagccaga aaagaggtgg tttctggacg
    49981 tattccttcc tgaagtcggt tttctggcgt tgttgttaag tctcggggtc aacgtgttga
    50041 ccccgttgac ggcctttgcg ggagtgcggt tgcgctggcc tgccatgatg cgactcactt
    50101 gcatcggcat tctggcgcag ttcgcgctcc tgctgctcgc ctttggcgta ctgacgtatt
    50161 gttttctcat cagcgatttc tcggtcattt atgtcgccca acatagctac agcctgctgt
    50221 cgtgggaact caaactggcg gcggtgtggg gcggccatga aggttcgctg ctgctttggg
    50281 tgctgctgct ttccgcctgg agcgcgctgt ttgcctggca ttatcggcag caaaccgatc
    50341 cgctatttcc gctgacgcta gccgttttat ctctcatgct cgccgcactg ctactgtttg
    50401 tagtgctgtg gtccgatccc ttcgtgcgga tatttccacc agcaatcgaa ggccgcgatc
    50461 tcaatccgat gctgcaacat cccggtctta tctttcatcc accgctgctt taccttggct
    50521 atggcggttt gatggtggcg gcgagcgtgg cgctggcgag tttactgcgc ggcgagtttg
    50581 atggtgcctg cgccagaatt tgttggcgct gggcactacc tggctggagc gcattaacgg
    50641 cggggatcat cctcggttcc tggtgggcct attgcgaact gggctggggc ggctggtggt
    50701 tctgggatcc ggtggaaaac gcctctttat taccctggct ttctgccact gcgctgctgc
    50761 acagtttgtc cctgacacgc cagcgggaga ttttccgcca ctggtcgctg ttgctggcga
    50821 tagtaactct gatgctgtcg ctactgggta ccttaattgt ccgttctggc attctggttt
    50881 cggttcatgc gttcgcactg gataacgtcc gcgccgtgcc gttgttcagc ctgtttgcac
    50941 tgattagcct tgcgtctctg gctctgtatg gctggcgagc gcgggacggt ggtgcggtgg
    51001 tgcgtttttc ggggttatcg cgggaaatgt taatcctcgc tacgctgttg ctgttttgcg
    51061 cagtgctact gatcgtgctg gtgggaacgc tttatccgat gatttacggt ctgctgggct
    51121 ggggacgcct ctccgttggc gcgccgtatt ttaaccgcgc gacgttaccg tttggcctgt
    51181 tgatgctggt ggtgattgtg ctggcgacgt ttgtctctgg caaacgcgtg cagcttccgg
    51241 cgctggtagc tcatgcgggc gtgctgttat ttgccgcggg gatcgtggtt tccagcgtca
    51301 gccgtcagga gatcagcctg aatttacagc cgggtcagca ggtgacgctg gcaggataca
    51361 ccttccgttt tgagcgcctc gatctgcaag ccaaaggcaa ttacaccagc gaaaaagcga
    51421 tagtggcact gtttgaccat cagcaacgca ttggtgaact gatgccggag cggcgttttt
    51481 acgaagcacg tcgtcagcaa atgatggaac cgtcaattcg ctggaacggc atccatgact
    51541 ggtatgcggt catgggtgaa aaaaccggag cggatcgtta cgcttttcgt ttgtatgtac
    51601 aaagcggtgt gcgctggatc tgggggggag gattgttgat gattgcgggc gcattgttaa
    51661 gcggatggcg ggggaagaag cgcgatgaat aaagggcttc tcacgctact gctgttattt
    51721 acctgttttg cgcgcgctca ggtcgtagac acctggcaat tcgccaatcc acaacaacag
    51781 caacaggcgt taaatattgc cagccagtta cgttgtccgc agtgccagaa tcaaaactta
    51841 ctggaatcca acgcgccggt ggcagtcagt atgcgccatc aggtttacag catggtggcg
    51901 gaggggaaga acgaagtcga aatcattggc tggatgaccg aacgctacgg agattttgtt
    51961 cgctataacc caccgttaac gggtcagacg ctggtgttgt gggcgctgcc agtggtgttg
    52021 ttactgctga tggcactgat cctctggcga gtgagggcga agcgatgaaa cagcccaaaa
    52081 taccggtgaa aatgctgaca acactcacga tattgatggt atttctctgt gtcggcagtt
    52141 atctgttaag cccaaaatgg caggcggtac gtgcggagta tcagcgtcag cgcgatccgc
    52201 tacatcagtt tgccagcaag caaaccccgg aagcgcagct tcaggcatta caggataaaa
    52261 tccgcgctaa tccacaaaac agcgaacagt gggcgttact gggcgagtat tatctgtggc
    52321 aaaacgatta cagcaattcg ctgctggcgt atcgtcaggc gttgcaacta cgtggtgaga
    52381 acgctgaact gtatgcggcg ctggcgacgg tgctttatta ccaggccagc cagcatatga
    52441 ccgcccagac tcgcgcaatg atcgataaag ccctcgcgct ggactctaac gaaatcaccg
    52501 ctctgatgct gctggcttcc gatgcgttta tgcaggcgaa ttacgcgcaa gccatcgaac
    52561 tatggcaaaa agtgatggat ctcaactcac cccggatcaa ccggacacag ctggtcgagt
    52621 cgattaatat ggcgaaattg ttgcagcgga gatcagatta atcatctggt gatgaagatt
    52681 atgttattga gttgtataaa aaataatcga atgaacatat gccaaaaata atcaccaatc
    52741 agtattattg cagattaaac aaataaaaat ctttccataa caaatggtta ttcattaatc
    52801 ctgccataaa ctcgcgttat tatgcattaa tgcagcgaaa agctctgttg ttaaagggtt
    52861 gcgcaacata ccgcgcaaat gatactgatc ataagcgtta aaaaaatcta caaaccaacg
    52921 caacacaatt catgccctgg cagtatgtca cgttctcgcg tttctgaacg gggaacggcg
    52981 ctccattgag gaagttattc tgatgaaaag tataaaattc agcctggcct ggcagattct
    53041 gtttgctatg gtgctgggca ttctcctggg aagctacctg cactaccata gcgacagccg
    53101 cgactggctg gtcgtcaatt tgctctctcc ggcgggtgat atcttcatcc atctgatcaa
    53161 aatgattgtt gtgccgattg tgatctccac gctggtggtg ggtatcgcgg gtgttggtga
    53221 tgccaaacag ctcgggcgta ttggcgcgaa aaccattatt tacttcgagg tgatcaccac
    53281 cgtcgccatc attttgggga tcactctggc gaacgtcttc cagcccggtg ccggggtgga
    53341 tatgtcgcag ctggcgaccg tcgatatctc gaaatatcag agcactacgg aagcggtaca
    53401 aagcagttcc cacggcatta tgggcacgat tttgtcgctg gtgccgacga acattgtggc
    53461 gtcgatggcg aaaggcgaaa tgctgccgat catcttcttc tcggtgctgt ttggtctggg
    53521 gctttcctcc ctgcccgcga cgcatcgtga accgctggtg accgtgttcc gctccatctc
    53581 tgaaaccatg tttaaagtga ctcacatggt gatgcgttat gcaccggtgg gtgtgtttgc
    53641 gctgattgcg gtgacggtgg ctaactttgg tttctcttct ctgtggccac tggcgaaact
    53701 ggtgctgctg gtgcatttcg ccattctgtt cttcgcgctg gtagtgctgg gaattgtggc
    53761 gcgcctgtgc gggttaagcg tctggatcct gattcgtatt ctgaaagatg agctgattct
    53821 ggcgtactcc actgccagct ctgaaagcgt actgccgcga attattgaga agatggaagc
    53881 ctacggagca ccggcgtcga tcaccagctt cgtggtgccg accggttact cttttaacct
    53941 tgatggttcg acgctgtatc aaagtattgc cgctattttc atcgctcagc tgtatggcat
    54001 tgacctgtcc atctggcagg aaatcattct ggtgctgacg ctgatggtga cctcgaaagg
    54061 gattgctggc gtgccgggcg tgtcgtttgt ggtgctgctg gcaacgctgg gtagcgtagg
    54121 tattccgctg gaaggcctgg cgtttattgc tggtgttgac cgtatcctcg acatggcgcg
    54181 tactgcgctg aacgtggtgg gtaatgcgct ggcggtgctg gtgattgcca agtgggaaca
    54241 caaatttgac cgcaaaaagg cgctggctta tgagcgtgaa gtgctgggca aatttgataa
    54301 aactgcggat caataattga agattgccgg ggttatccac ccggcaatgt gtggatgcct
    54361 gatgcgacgc tggtgcgtct tatcaggcct accttaaccg ttggttaatt tttcaaactc
    54421 ttcacacccg gtatcaaacc cttccataca gctcaggttc aaccagtgca gcgccttttg
    54481 tttgttcttc tcgataaaac cttcctcacc gtttaagaac atcattcccg cccagtactc
    54541 ggaataaccg gttcgggaaa ttgcagagct gcgtttgaaa taccaggttg ccttgtcatc
    54601 gtcggcctta atgcccacgc cgttggcgta aatcaaacca agcagcattt gggcatcgac
    54661 ggcggagtca ttctccagat cttccgaggc gttttctaac agcgaaatcg cctttggata
    54721 atccggttta cccgcttgag tatttaccag aatatgcgcc agcgttactt caccttcttt
    54781 actgcccgct tgcgccgctt tctctgcaag aacttttgct tgtggatagt ccagactgac
    54841 cggattggtg attttgatct gcgccagcag cgcgcaggca tcagcatcac cgctgtcggc
    54901 ggctttctgt gcccaatatt cggctttgct caaatcgccg gagctaaacc agctgtcggc
    54961 aagaaaatat tgtgcgcgtc ggtccccggc ctctgctgcc tttaaatact ggctgccagg
    55021 ctcggagtcg ttcgcgtggg caaagaatgt caaaaacaac atcaatgcga taattttttt
    55081 cattttgata atacgattgg gtagggagta accagtataa agagttcgaa tggataaaaa
    55141 aacagcctcc gaaaggaggc tgtagaaagg actgtattac gccagtgccg cttcgcgcag
    55201 gcgagttttc aacttgttgt actcgtcaat cacgtactgc tcggcggcgc gctgatcggc
    55261 gatcggctcg acgcgcacgg cgcagtattt gtattccggc gttttcgtaa tcgggcttaa
    55321 gttttcggta accagctcgt tacaggcacc aatccaccac tggtaggtca tgtaaatcgc
    55381 ccctttgttc ggacgatcgc tgacctgcgc acgggtgata attttgcctt tacgcgagtg
    55441 cacccaaacc aatgcctcat cttcaatacc cagacgtttg gcgtcttcgg tattgatttg
    55501 tgcgtagcca ggttcatcag ccagcgccgc cagtgccgca cagttaccgg tcatcgaacg
    55561 gcaagagtag tgaccaactt cacgcaccgt tgacagtacc atcgggtact cgtcggtgag
    55621 tttgtcgatt ggcgctaccc agtcgcaggt gaagaactgt gccagaccgt tcggggtatc
    55681 aaacttctct ttaaacagat aagaagtccc ctgatcggca tctgaagtat cgcggcaagg
    55741 ccactgaatg aagcccagtt cgcccatttt ctcgtaagtc gcaccgtaga aatccgggca
    55801 cagatgacgc aactcatccc agatctcctg ggtgttgttg tagtgcatcg gataacccat
    55861 acgggtggcg atttcactga tgatttgcca gtccgttttc agatcccatt tcggttcaac
    55921 tgctttgaag aaacgctgga agccacggtc agccgcagta aacacgcctt catgctcgcc
    55981 ccacgacgtt gacggcaaaa tgacatccgc cgccgacgcg gttttggtca taaagatgtc
    56041 ctgaacgata accagttcca gatcttcaaa ggctttacgt actgccgaca gctccgcgtc
    56101 agtttgcagc ggatcttcgc ccataatgta cgcggcacgc acttcgccat gcgctgcgcg
    56161 gtgcggcagc tcgctgatgc gataaccggt atgagcaggc aggctttcca cgccccaggc
    56221 tttggcgaat ttctcgcggt tagccggatc tttcacgtac tggtatcccg gatacgtatc
    56281 cggcagcgcg cccatatcgc aggcaccctg cacgttgttc tgaccacgaa ccgggtttac
    56341 acccgcatgc ggcttaccga ggttaccggt cagcatcgcg aggctggtca gagaacgcac
    56401 ggtttccacg ccctggtaga actgggttac acccatgccc cacaggatgg cggcgctttt
    56461 cgcctgggca tacatccgtg ccgcctgacg aatctcactg gcgctgacgc cggtgatatc
    56521 ttcaaccgac tccggcgtgt agccttcaac gattttacga tactcttcaa agccttctgt
    56581 acgggaagcg acgaacgctt tgtcgtacag attttcttca ataatgacat ggcccatcgc
    56641 attcaacagc gcgatgttcg agccgttttt cagtgcaatg tgcatgtcag caatgcgcgc
    56701 ggtttcaatt ttgcgcggat cgcagacgat aattttcgcc ccgttacgtt tagcgttaat
    56761 tacgtgattc gccacgattg ggtgggaatc cgccgggttg tacccgaaaa cgaacactaa
    56821 atcggtatta tcaatttcgt taatagcatt gctcattgcg ccattaccga ccgattggtg
    56881 cagacctgca accgatgggc cgtgtcagac acgagcgcag cagtcaacgt tattggtacc
    56941 aataacggcg cgcgcaaatt tttgcattac atagttggtt tcgttacccg taccacgcga
    57001 ggagccggtc gtctggatgg catccggacc gtacttctct ttgatggcgc tcaggcgctc
    57061 ggcaacgtaa ttcagtgcct catcccagga aacaggttcg agtttgccgc cacgctggcg
    57121 acggatcatg ggggttttca ggcgcggggt caggatctgg gtatcgttaa tgaaatccca
    57181 gccataataa cccttcagac acagggtacc ctggttggtt ttcccctgcg ctgcctccgc
    57241 ccggacgatt ttgccgttat cgacgaccag gttgattttg caacctgatg cgcaataggg
    57301 gcaaaccgtg acgacttttt tcatcggtct cgctccagtt aatcaaatca cgcatacgcg
    57361 ctctcgacta cagtatgcat cttttatgcc acattttatt tggggtcatt ccctgatatt
    57421 acgggcacta tttattcaaa actctgacga aaaacaggct gtcgtcagtt ttgacgtgac
    57481 gaaacgaaat accgcgtgac agccatcacg cggcagacat tttatttttt ctcgacgacg
    57541 ggagccgcct gatacccgcc gcccagcgat ttcatcaact gaatgctttg gatcacccgg
    57601 cggctgtcca gcatcagtaa tgacatctct tcggcaagta ctggcaaccg ggcttcagtg
    57661 gcctgtaagc ggctggttaa gccgcgctga taggcggcct cggcagcgcg ctgggtgaag
    57721 cgcgttgctt ccacgcgttc agcctgcatt tctcgctcgt cgttgagcgt ttgcagacgc
    57781 gtgccgttga cggcaacgtc acgcaccgcg ttcagtactg actggttgta acgttcaatc
    57841 atcatgttgc tggcggcgcg cgtgccttcg agattggcat tcaaccgtcc accgtcaaac
    57901 agcggcaatt tcagacccgg gatgaagttg aactggcgac tggttttttt gaataaggta
    57961 tccaggtgga tggcatcaag gccgaaaaac gctttgatat caaagctcgg gtagaacagc
    58021 gcccgcgcgg aatccacctg atctaatgac gcctgaacat accagcgcat ggcttgcaga
    58081 tccgggcgtc tggcgagcaa ctcataagag agtgtcgccg gaatgccggt ctggactcgt
    58141 ggtaatgcca ccggtttgat ctccggcata tcgctggccc ccgcgccaat caatgcacgc
    58201 agagattctc gcgtttcggt gatttgccct ttgacggcag caatttgttt atcgaccgcc
    58261 agaatctgtg cccgcgcgcc gtggaaaggc acttgtgctt ccagaccgtg cgccacttta
    58321 ctctggtgcg ctttcaccgc gtaatcaatc acatcgcgag tttgttctaa cagatcgagc
    58381 atctgatagc tggcctgcat actgtaataa agctgcgcta cgcccgtggt cagcgatagc
    58441 tctactgctg cggtttctgc cagcgcggca ttatgcgcgc caatggcggc ggcaaccgct
    58501 gagcgatgca caccccacaa atcaagatcc agtccggcaa acaaacctac tgtggcttcc
    58561 gtatagtacg gcccgtccat acccagtgcg ggcgcatcca tcgcataagg gcttaaaaag
    58621 ccgttcgccg agacgcgttg gcggttgagc atccctaacg ctgcgacctg taattgtgaa
    58681 ccggcatcta acaaatcggc ctgggactgc gctttttctt cccgcagttt cgcttcggcg
    58741 agggtgtgtg aaccacttag cgtccgttgg atcagcgcat ccagctgcgg gtcattgagt
    58801 tgtttccacc actgcgcctg cggccagccg gagctggcaa gatgaatatc gtcggccagt
    58861 ttgatttgtt ccggtttgag ctgttgatga ggtgcagaat ccttacgtac cagggcacag
    58921 ccggaaatca gcgtcgtgct gccgagaatg ctgcacaaca gcagacgtga aagttgacga
    58981 ttgatcattg cgtggctcct tgtgcctgtt ccgtggcggg cgttaatgcc ggggctgtcc
    59041 agtctggcaa gcgggcgaaa agctggcaga cgtgttgctc ctgttttaat aaggtgggca
    59101 ggatggcctg cgaggcgggt gtctcttcaa gcgttatttg aggagaacgg ctgagtgcgg
    59161 ttgccagacc ggcagcgtat ttctccaggg cgtcggcaaa ctgagctgct ctgtccggct
    59221 gtagtgcgtt atccagcgcc tgcgccgagt tccaggtggc atcccaggcg tgaagaatat
    59281 cgcggccctg acgaataacc gtttgcgaac gttcaatcag taaggcgcgt tcttcgctgt
    59341 ccagttgacg ctccagcgcc acgcgttggc acatctcttc acaggcatta aacgccgcat
    59401 gcagaccgat acgaatttgc agataagtgc gcagagccgt gacttcctgc tggcgtggga
    59461 tccgcattac tttacttaac atacccagcg tgccagccag tttttgcggc agtgtgcgcg
    59521 cttcactttc aggccagaca aaggtgtaaa tcaccgcgga caccacggta ccaatgatga
    59581 tacccatggc gcgatcgcga atttccacca ggtcgtacac cgggccaaaa acgttttcga
    59641 gcgtggcgag cgcgaaggtg accaccatct gtgtgccgat ataagaagag cgttcagagc
    59701 tggtggcaat ccatgcgccc aacaggaaaa tcggtgccag cacaaacagc aattcgacaa
    59761 tattgtccag ccagggcatg accagtagcg tgaataacag cgccagaatc gcgccgcaaa
    59821 aggccccgcc aaaacgcagc accatcttct ggtacgacga accgacattt gggttagcga
    59881 cgatcacgca tgtcagcata caggtgtgaa tgccttccca gtccacgccg ctgtagaagg
    59941 tgtaacagat caaacaggcg agcagtgttt ttaccgcgta gcgcatatag tctggattgg
    60001 taaaagcatc ggcgaccatt gatggcggtt tggcggcggg cgttggcggc gtattcgggt
    60061 ccatctgacc cagttgtaac aacgtctggc agatattctc caggttacat tcccgtgccg
    60121 ccatcgcttc actttcagtg atccgccagt cgctttgcca gcactgacct tcagcaacgg
    60181 catgctgcag cttattgatt tctgaggcta atttttgccg aaattcaata attgcctgag
    60241 aatcagcaaa agaggtggga tcgtagcgat tcagcgtcga gtaaatgtag gttaccgttg
    60301 ccacgcagct ttgccaccat gcgctttgag ttcgccagtt ggcatcgtcc gcgagacaaa
    60361 agacattcag tttttgcagc gccagcgcct ctctttcaat ccgcgtttcg ggtagcggtg
    60421 cgaggctgtc cgtcaggtga ctaatggcat catcaagccg atcattaagc gcttgatgca
    60481 tttgcgtaat ggcacgactg ggaaaccaca gcacgccgat taacgtcatc agcaaggttg
    60541 gatagaggcc aacaacgata caccacagcg ttaagcgcac gaccacttcc ggatagtcga
    60601 gcatggcggg gaaggtttgc ccgtaaatgg cgacaatggc gacagcgaaa aagaccagtc
    60661 ccagccgatg ggtgcgcatc aaaaacatac agcccatcag gatcggcccg gcgatgatca
    60721 atcggatcaa cggttcgccg tatgaccatt tgtagatcaa aaacaggctg ccgatctcca
    60781 gcaccgtggc aaccacaaac aagatcgcga caaatttggt gtaaaacgcg ttcgactgaa
    60841 taccgtaaaa cagcactgcc agcgataacg ccacaaaagg gatctcaaaa gtcatcgaga
    60901 tcagaatcac cagcaggcag cctacccaga gttgcacgat ctgcggcacg cggccgggtc
    60961 gccgctcgct taactcttca tgaaagaacg ccagcagcct gaccaccggt aatggcaggg
    61021 agttgagcgc gctcattatt gcggctcaag attagcgact gccgaagcgc cgatgcggat
    61081 catttccggg tcaggtttat cgaccatgat tttgaccgga aaacgctggg caacgcggac
    61141 ccagttaata gagcgagaaa ctttcggcag gccgcccagc accaggccgc cgtcatccgg
    61201 tagcacgccg tagccaatcg aatccacttt accctcgaag gttttgccgc tgtcactcat
    61261 caggcgaatc gttgcgggtg tacctgagcg aatatttttc agatcggttt cgcggaagtt
    61321 ggcgatcacg taccagtgac gagtgtcgat tagggtaaaa atagggcgca tggcagaagc
    61381 aaattgcccg acggaggttt tgagggaaat gacccggcca tcaaacggcg cgcgaacggt
    61441 cgccatttcc agatgaagtt tggtcagggc aatatccgct tcgaccgccg cacgttgcgc
    61501 caccagcgca tcgacgccgc tgacggcgct ggcggctgac tgcgcctgta acaatacggc
    61561 attaagatcg gcttctgcgg cgcgctgcgc agttcttgca cggtcaacgt cttccgctga
    61621 gacaaaaccc tctttcagta atggctcggt gcggcgtaat gtatctgtgg cctgtttcgc
    61681 ggcggcacgg gctttttcta ccgtggcatt aatcgagtcg gcaccaaact gttgcgcatc
    61741 aacgctacgc tgggtgagca taatttgctt atccagcgcc gcgagggagg cttcagcttt
    61801 cgccagattg gcttcgtacg ggcgcgggtc aatgcggaac agcaaatcgc cctgtttgac
    61861 tgcctggttg tcggtgaccg ccagttctac aatgcggccg ctgacttccg gcaccacatc
    61921 aatggtatct gctgacgcgt aagcgtcatt agttgatggc gcactgtcta cgcgccagat
    61981 aacgaaaaca agggcaacca gcgccaatgc aaccactaac agagcaggga atttactgcg
    62041 aggagctttt ttcggcgtac tttccatgag cgcgtctcaa atagattaga aaaatgccag
    62101 ccagaacagc atggcgtaga gagcaaaaag ggcggtataa ataattccga caaatgccag
    62161 attgatattt gcccgctgaa taactcgcct cgtgatgagc atcaaaataa ggctggcaat
    62221 ggcgcagaaa aaccagctgg gataataagc accgatcacc gggattgccg gagataatga
    62281 gcatcccgaa accatgaaga cgggaattat ccaggctgtt tttttgagtt gcatcgccat
    62341 gcgagagaga actgttggca tattaaatac atcctgtctg ctgcgaacaa tatctgtagt
    62401 gaatatcaat atccattgaa ggcgtcagta tggctatttt ctcgatgaat aaaaatagct
    62461 ttattcagcc accgcattca taacttatat attttattta ttaattggct gtatatattt
    62521 ttaaaataaa tgcttttggg ggcttcagcg cccccaaaag cgagtgacat taatttgggg
    62581 ttacgatatt cacccacaaa tcgaaattat ccagacaggc gataatttct tccagtttcg
    62641 cgccattgcc aataattttg gctttcttcg cttttaccag atccgccatt ttggcttgcc
    62701 cggtcagcac ggcgtgcaga tcttcacggc tgatgtagaa agaggcgtca gcttgggatt
    62761 gcagtgtttt acggtagtta agcacgctat cgttcagcgt caggttgagg ttatcgccat
    62821 tgctcatatt gaagttcagg ctgatatttt tacccgcggc tttcgcgcta tcgaggcgaa
    62881 cggacataaa gtcgaacagc atttcgaccg acatcccgcg aatggtgtcc ggggaaccgg
    62941 tggtgccgtg gctgaactta tgcacccctt cgcgcagctc tttcgcgccg gtcaggtaga
    63001 aaccgcgcca ggtggcggac tcggcctgat agcccagctg ttcaaagtta ttcgcttgca
    63061 gattcttcgc gacctggtca cccgggttgg cggcaatcac ctgtttcagc agttctgccg
    63121 accagcggta atcaccttgc ttgttcgctt cttgcgccag gttgatgaca cgggcagaac
    63181 cgcccagcgc ctgcacgtaa cgtttaccca tctccacctg accatacgga tgcaggttag
    63241 ccgggttacc gtcgtaatag ccaagataga agttatacac cgcgcgggcg ttgtggctga
    63301 cagaaccgta atagccacgg ctggcccagt tattggcaag tgcaggcggc agtttaatca
    63361 tgtcgccgat ttcattcatg gtgtagccct ggttcgccag gtgcagggtc tggtcgtgaa
    63421 tgtacttgat agtatcgcga tatttaccaa tataatcatt gatatgctta ttgccccaga
    63481 ccggccaggt gtgcggcata aacaggactt ccgcgtcgtt accccacata tccagcgttt
    63541 cgttcagata ctcggtccac ttgctggtgt cgcgggtttt cgcgccgcgc agagtgtaga
    63601 agttgtgcag ggtatgcgtg gcgttctcgg cggtacacag cgctttcagg gccggaatat
    63661 agaagtgcat ttcggctggt gcttcgctac ctggggtcat caggaagtca aactccaggc
    63721 cgtcgataat catcttctca ccggttttgg tgatggtttt gttcggtgcg atgatgctcg
    63781 ggtagcccga tgccagcgtt acgccaagac cattgccgat gttaccctga gggttatgcg
    63841 ccagcagcag gccgtaggag taaagtgcac ggcggctcat aatattaccc gccagtacgt
    63901 tttcgctgat ggcttcgtcc ataaagccag caggggcgat aacctgcact ttaccggatt
    63961 tcacatcagc ttcagagata atgcctttca cgccgccata gtggtcggta tggctgtggg
    64021 tataaataac cgcaacaatc ggtttttgcg ggcgattttg gaagtaaagg tcaagggcgg
    64081 cttttgcgct cggtggcgtc accagcgggt cgatgacaat aatcccggta tcaccttcga
    64141 taaaggtgat gttggagata tcctgaccgc gaacctggta cattctgtcg gtgactttaa
    64201 acaggccgga aataccgttt aactgcgact gacgccacag gctagggtta acggtttcgg
    64261 gcgcgggcgc attgatatca aatttataat cttccccgcg ataatatggt ttgccattag
    64321 catcgcgcag aataccttca tccagcagcg gagcgataaa tccacgctga gcgtcgtcaa
    64381 aatcctgacg atcgctaaac ggtaatgatt tggcgtaatt ctgattaacc tgttgggtat
    64441 attgagtggc ggctttcgct tcatctgttg cgtaagcggt atttaccatc atgccgctgg
    64501 cggcagttaa cgcaataaca attctgctca aacggaataa ccgagagtta ttcatataaa
    64561 tctccattta tcatttatgt cgtaaatatg taatccatgc aaaatacgtc atttaaagct
    64621 ggcgttatca taattgggaa attgcgactt taataagtgg aagtgtgagc ggaacgatcc
    64681 attttattag gcatttgtgc tgaagggggg gaattacagt agaactatca ttgctggaaa
    64741 taatatgcac cgttttatgt atttattata tatattgtac ctgtcgttgg cacagcggga
    64801 cattgcgcta ctgtgccaac gctttactca tgggacttta gcacaggact gtggcaaaaa
    64861 acgttgatgc gccaatattg ctgccccctg agcgccatta aagtcagaag atgaggcggc
    64921 aataaagcgc acgacctgat gcggcagtgg acggcgcagg tacttttggg tcatggcaat
    64981 gagagtctcg cgtggaaagg cgggcatatc catcacgcca ccgcccagaa tcaccgcatc
    65041 gggatcgaac agattaatgc tggtggcaat ggctcgagcc gcgttttcaa gcagactctg
    65101 gacgaaagga gcgttttccg catggacgaa aagatcgctc aatgggtaat ttcggggctg
    65161 ttgttcgtac cagcgtctta gcgccattcc agagcaattg gtttccaggc atccaggatt
    65221 gccacacgcg cagtgttggg tcatatctcc cagggggata tgacccagtt cgcctgccac
    65281 accgtgtgca cccgtccacg gcgcaccgtt catccacact gcgaacccca tccccgtacc
    65341 gagataggcc gccagaacca gttgttgcgt aaggcggttt tctactacgt cccaggagag
    65401 ttgcaggtta acgtcgcggg aaaactcaac cggacaattc agcgtatttt cgagcttatc
    65461 ggcgagatca tataaatccg ccgctgttaa cggcaggtta ggcgtagaaa taatggtgcg
    65521 tttatcttta ctgaccagcg ccggaaatcc catcaccaga ccatgacagc gagcgttaaa
    65581 gcgtctgagt tgctcgtcaa tcatttcgcc gatacccgac accaggccgg gagcaatgac
    65641 ttctgcggtc cgcttttttt cgcagtgtag cgtttcacct tctgctgtcc gcagacaaaa
    65701 gcggatatgc gttgccccca tatccacgcc cgctacgacg ttatgctgtt tttgcatgag
    65761 gctgtacctc gctttttgca gccagaatct gcgcggtcat aattctccat gcttcgtcga
    65821 tattttccgc atgattaaac aggccggaag tgccgacgat aaagacatcc gctcctgccg
    65881 ccattagttt ttcgtaagtt gcctggttgc aggaaccgtc cacctcaatt tcgtactcca
    65941 gaccttctcg ttcacgccat gccttcagtt cggcaagttt atccagcatt tcaggaatga
    66001 acggttgtcc ggcaaagccg ggatcgacag tcatgaccgt aattttatcg gccttatgga
    66061 tatagtattt catggcctca actggcgtct ccggattaag gatcagcccc actttcatgt
    66121 catgacggcg gatttcatca atcaggcgga acgcctggcc gttgatggtt tccggatgca
    66181 gagtgataaa atctgctccc gcacgtgcca gttgagcaat gtaatcctgc ggccgcgtca
    66241 ccatcagatg acagtcgagc ggtttagttg ccagtttttt aacctgactt acgaagaacg
    66301 gtgagagtgt cagattgggg acaaagtgac cgtccatgat atcgatgtgg aagtaatcgg
    66361 catggctgtc gataaattcg atctgttctt taaatttcag cagatccata cacattaacg
    66421 agggggagat tttcatgttc aattccttac ttactgataa gacggtcaag ggcgacagcc
    66481 gcgataatta atccgcccat caccaccagt tggtaatagg tttgtacctg caaaatattc
    66541 agaccgttgt tgatggtgcc gatgatcaac ccgccaatca ccacagagaa aatgcgcccc
    66601 ttgccgccga agaaactggt gccgccaatg atggcgctgg caatggcata ggtttcaaaa
    66661 cccataccgg caagcggttc tgcggcaccg agtcgtgcag ttgagacgac gcctgccaga
    66721 cctgcacaaa caccggagat gataaacacc accaggatgt ggaatttcac gtcaatcccg
    66781 gaatagaacg ccgaattttt gttgccgccc agtgcgtaga tgttgcgccc gagccgcata
    66841 cgcgttgtca gaaaccaaag gatgagcgcg acaattagcg agaagataac ggggacaggt
    66901 atcccaatta cgctggcggc aaagaagttc acgaagtcaa aggagaagcc gtataccgag
    66961 ttggcatcgg agatcaccag cgtgatccca cggaaaatcg cgttggtgcc aagggtgatg
    67021 atgaacgggt gtagccccgt ccagttgacc aggcagccgt tgatcgcccc cagtgcgccg
    67081 ccaaccagta caccgccaat catcgctgcg agaaacgggt caacacctgc caacatcagt
    67141 ttggcggtca ccataccgga aagcgccaga atcgcgccaa ccgagaggtc gataccagcg
    67201 accaggatag cgaaaaactc gcccatgccg atcaataccg tcacggagct ttgaacaaaa
    67261 atctgggtaa tattattggt ggtcagaaaa tattctggcg acagcgaacc aaaaatggcg
    67321 acgatgatcg ccaggataaa aaaggtgccg tatttatccc agaacagcgc aaagttgaac
    67381 ggtttcttct cgctcgcttc gctttttact cttgtggtaa agcccatgcc ataatctcct
    67441 cttcgctcat gtcatcgcga ttcgtcagga tttgcgtcag tcgtccttcg cagaacacgg
    67501 cgatgcggtc gcagacggtg ataatttcag gtagttcaga tgacaccatc aggatgactt
    67561 ttccgtcgtc cgccagttgg cgcatcactt tgtaaatttc ggctttcgcg ccaacgtcga
    67621 tgccgcgggt aggttcatcg aaaataatca cttccgggca acagcacagc catttggaga
    67681 tcaggacttt ctgctgattt cccccggaga gttcggtgat attctggttt accgaatgac
    67741 atttcagcgc cagcagttcg cgttgatttt cagcggtacg ttgctcgtca acttcatgaa
    67801 acaagcccat cgcgccttta tagccgccgt ctttcagact gcggctgatc gccatgttct
    67861 gagcgatgga aaagttgggg aaaaaaccgt tatcccggcg gctttcagtg atgtaagcca
    67921 tccctttttt cacggcatcc aggggggaac gtggagagat atctttgcca ttaagacgga
    67981 tttctccgcc agcgcgttta tcaacgccaa acagacaatt catcagttca gtacgtccgg
    68041 aaccgaccag cccggcaaag cctaatattt ctccccggca gacgctaaat gagatatccc
    68101 ggaccttttt tctgtcacgg ctggtgacgt tgcgcacctc aaaaaccgtt tcgtgcgcaa
    68161 ggttgctgac attctccttc atcgcgttaa aacggttttg cagttcgcgg cctaccatca
    68221 gacggacgat atcgtcattt gacacatcgc tcaccatgcc gctgcaaacg ctgctaccgt
    68281 ctttcatcac cgtatagcgg tcgcaaatac ggcgaatttc cgccaactta tgcgagatat
    68341 agacgatggc cgtaccctct ttacgcaact gattcatgat cagaaacaga tagtccacct
    68401 ctttattggt gagtgaggag gtgggttcat ccatgatgat gactttggca tcgagcatca
    68461 gcgttttggc aatttccagc atctgcttgt ggctgataga taaattcgcc actttctcat
    68521 ctagatcaac tttcaagccc acgcgtaata acatcatggc ggcgcggaca cgcatttctc
    68581 gccagtcgat aatattgacg ccacagattt ttttcgtcag atgacgacca atatataaat
    68641 tttccagtac ggttaattca tcaataacgc tgagttcctg ataaataatc ccgataccga
    68701 gttgtgccgc taatttatga tccagcttgt tatagctaat gttattaatg gtaatggtgc
    68761 ctttggtcgg ctcatgtatt ccggataaaa ctttcattag cgtggattta cccgcgccat
    68821 tttctcctag taatgcatgt atttcaccag gataaaccgt taaattaacc gactttaatg
    68881 cgtgaaccgg accaaaggac ttgccgatcc ccgccatcga tatatatggc gtggccataa
    68941 ataacctctc tgcacgaaat atcgtattat tgcctgatgg ctacatttat caggtctacg
    69001 ttcaggttat tttgttggcc ggataagaca cttcattagc atcatcatcc ggcatcattc
    69061 agttttattg agtgaccagg attgaatcga ccagtttaaa ttccggtgct ttatccagcg
    69121 ggataacctt gccggatttc tcagcgtcaa ccatcagctt cagacccgtt gcgccgatat
    69181 ccgccgggtt ctgggcaacc gtcgcggtca tttgtccggc ttccaccatt ttgcgggctt
    69241 ccggaatgcc atctgtaccg acgaccagca cttttcccgt ttttccggcg tttgcgactg
    69301 cctgagcaac acccattgcc atcgtgtcat tcgcgcaata gatcgcttta atattcggat
    69361 tacgttgcaa cacgttagtg gcgacatcca gtgctttaat gcggtcccag tcggcaggct
    69421 ggctggcgac aagcttgatc tggcttgctt ttttgaaggc ttcggtggca ccattacgac
    69481 gcgcttcacc ggaggcgtta ccggctttac cctcaatgat tgcgacttca ccaccttcag
    69541 cgcccaattt gtcaataatg aacgacgcgc ctttcgcccc gacagcaacg ttatcggtgg
    69601 tgacaaaagc ttccacattg ccgccagctt ttttcagatt atccatgtcg attttttcat
    69661 cgagattaac cagataaatg ccttttttcc atgcgcgggc gacaggcatg accagattca
    69721 ctgaggataa tggagcgaag gcgatacctt tgtaattttt attactgaga tcttcaaata
    69781 actgcaattg agattgaaaa tcgccttctg aaggagaggc aaaaatatca acgctgacgc
    69841 ccagtgtttt tgcttcatct tcaatgcctt ttttcatatc tacccaaaat gggttggaga
    69901 gggttttcaa tacgacagca tattcggcgg cagcaaaagc gctggttgac aacattaagc
    69961 ccacgagtgt gctgctgaaa tatttcagat atttattcat aatattgttc tcacggtaag
    70021 gggacaggta actccgcgat ggcggaatta cctttttctt atttcagcgc gcctggtgag
    70081 aagaaatcaa caattgcgcc ggttttttgc atattaatat tagcttgttc aatgttgagc
    70141 tgggcgacag agacaaaaaa agcgtccagc aaagttagtt gtaatattct tgccgaggca
    70201 ttacgaccta ataacggcgt ttccggggct ggtgagcaaa taatataatc ggccagtttc
    70261 gctattggtg aatggtagct atgcgttata caaataatct ttgccccgtt cttttttgcc
    70321 agttctacgg ccgcttttac atcactggtt cgcccggaat gggtcactac cagcacaaca
    70381 tctccttcct gtaacaacga agcggacatc atcatgatgt gagcgtccgg ataggcctga
    70441 cagcgtacgc caatgcgcaa gaacttgtgc tgtacatcag cacagatagc atttgatcct
    70501 ccggcaccgt ataaatcccg ctgtctggcc tgatagaaaa agcgggcggc acggtggatc
    70561 tcatcaacgt tgacgatcga ctgaccttcc ataatggtgc gtaaagtgat gttaaatacc
    70621 ttattcacca catcctgcgg cgcttcatca aaagccaact cggaaggcaa tacttgttct
    70681 gactgagaaa aataatcttc cagcgcactg cgtaagttac gaaagccgct aaaccccagc
    70741 agctttgata ccttaactat catcgcttca gataccgcca gagcttctgc gacatcttta
    70801 attgcgggtg cacaactcag gttaccgggt ttgagtaacc actccacgat gcggctttca
    70861 ttttctgtca ttccttcctg tttcattcgc aggtaaggcg ctaaccctat accgttcgga
    70921 agcgctgaat caaactctga ctggctcatc acgttctctt gtccttgaat tgaagacgcc
    70981 actatatcac cacagcaaga acagaaaaga tgctggctac ttcacaaaaa aataaaattt
    71041 ataaagtttg ttttgttgtg aaaaatggat agtggcttaa aattttattt cattcaatca
    71101 ctttaaaaac atgatgttat ttgtttttct ggttttttag atgatagatg ttgctggata
    71161 gtaagtgtct ttaacgctat tgcgctgtca gatgtaactc tgtaaaacag atcaggaagg
    71221 cggatttcat cgattttggg tgtttttaat tcatttacat cacaaatgtt ttttgattgt
    71281 gaagtttttg cacgaacggg gaagatgaat gaaaaagatt gcatttggct gtgatcatgt
    71341 cggtttcatt ttaaaacatg aaatagtggc acatttagtt gagcgtggcg ttgaagtgat
    71401 tgataaagga acctggtcgt cagagcgtac tgattatccg cattacgcca gtcaagtcgc
    71461 actggctgtt gctggcggag aggttgatgg cgggattttg atttgtggta ctggcgtcgg
    71521 tatttcgata gcggcgaaca agtttgccgg aattcgcgcg gtcgtctgta gcgaacctta
    71581 ttccgcgcaa ctttcgcggc agcataacga caccaacgtg ctggcttttg gttcacgagt
    71641 ggttggcctc gaactggcaa aaatgattgt ggatgcgtgg ctgggcgcac agtacgaagg
    71701 cggtcgtcat caacaacgcg tggaggcgat tacggcaata gagcagcgga gaaattgaga
    71761 ttcatccact acttgcatgg atgagtaatg attaatgtgg atagagtttc tttttgaggt
    71821 tggctaatga aacgctttcc gctatttctt ttattcaccc tgctcacgct gtccaccgtt
    71881 ccggcccagg ctgatattat cgacgatact attggtaata ttcagcaggc gattaatgac
    71941 gcttataacc ctgaccgtgg gcgagactat gaagattcgc gcgacgacgg ctggcagcgt
    72001 gaagtgagcg acgatcggcg cagacaatat gacgaccgcc gccgccagtt tgaagatcgc
    72061 cgcaggcagc tggacgatcg ccagcgccag cttgatcagg agcgtcggca actggaggat
    72121 gaagagcgga gaatggaaga tgagtatggg cgatgaggtt gggtatggag caggcatcgc
    72181 ccctcacccc aatggggaga gggagaaaac gagcgcaata ttcaatatcg agcacaatcg
    72241 gtcccctcgc ccctctgggg agagggttag ggtgaggggg ccagcgcgca cattccctct
    72301 catcacgcca ccccaatctc catcccatca aaccccacct caaaccctga cggtagtgcg
    72361 ttttccatca gccacgcatc aaactggtgg ctgatatggg tcagaatcac ctgtggcgag
    72421 cggataacct gattcagcgc aagcacggta tttaaatcac agtgattacg cggtgcatcc
    72481 gcgcgcggcg ggtgactgca atcaatcacc atcacctgcg gatgattatt gagtaaaaat
    72541 ttcagcgttt tttccggcag gccagcggtg tcagacagcc acgccacccg gctatgtgcc
    72601 gtttccagca gataaccgaa ggtcagtttt gagtggttaa gcggcagcgg cgtgacctgc
    72661 aacccctgca aatcaaacac cacaaacggt accactgtgt ggctgaaatc aagcaggccc
    72721 ggatgtttaa acagatcgtc gcagccctgt tcatccggcg ggccgtagac cgggatcaca
    72781 tcgcccacgc cccagcgtag cggaaatagc ccctggacgt gatccatatg gtagtgcgtc
    72841 agcaaaaact gctggaacga tccaggcgac cagcgatcgg tgagatcgtg ccgcccggcg
    72901 tcgatcaggg tgatcgcgtc gttaaacttc actacgccgc tgcatggctg gcggcgatac
    72961 tgcggcgagc gccgcgctct ggcgcaggcc gcgcactcgc agccccatgc cggaacgccc
    73021 tgtgcgccgc cagtgccggt gagcgtgagg gtcaggctca tgttacagcg ccttggtgaa
    73081 gcggaagtgg ctctgctcgt agccttcacg caggtaaaaa cggtgtgcgt cgtggcgctt
    73141 cacgttggtg gaaagctcgg tcatttcggc cccggcctgg cgggcttctt cttctgccca
    73201 tgccagtaat ttactgccga cgttcagacc gcgcgcctgc ggcatcacca ccagttcctg
    73261 aatttcgccg atccagttga catgatgcag atgaaactgc aaatgcaggc cgatcatgcc
    73321 gacaacttcg ccatccagca gcgccagatg gtagcgcatg tttgggtcgc gcaggttggc
    73381 gttaaagccc acgcgaaacg catggtggtc aaactccgcc tgttttagct cacaaatcag
    73441 cgcgtaaacc gcgtcggtat cgtactgcgt ggccgggcga agctcacaag caggcatggt
    73501 gtttctcctt ctgatggatc agcgtcagca gcgtgtcgac cgactggcgc aggctgccgt
    73561 cattgttgag cgtatggcaa tcctgtggag tatagcgggc ggcgcgcgcc aggcgggcgt
    73621 taatttcact ggcgttttca cggccacggt tttccaggcg ctggcgcagg atctccggcg
    73681 aaacctgtaa acagacgggc agcagcgccg attgatagcg cgcccgtgcc tgcggcaaat
    73741 gcgcgcgtga accgttgacg actacgtcga atccagcgtg cagccagagg tcaatctcga
    73801 tgccgacgcc ataatacaga ccgttagcgt gccagcttaa ggccagcagg ttttgccccg
    73861 cgcgggtaaa aaactcccgc tcactcaggg cgatatggtt ttcacttccg gcgctggcgt
    73921 cgcgcgtgat gtagcgatgc gccaccagta actgcgtttg ttcccgcagg cggagttccg
    73981 ccagcaggct gtctttcccg gagccggacg gccccattaa ccaaatcagt tttcccatca
    74041 tcagaacacc cttttaccct gacgccagac gtggtcgata tgaatgtgat tgccctggcg
    74101 atgcgccagc accaggtcag cgcgtttacc ctcgccaatc accccgcgat cctgaagatt
    74161 cagcgcctgc gccggatttt tggtcaccag cctcaccgcc tgcggtagcg taaagcggtt
    74221 gctctcgtca tcggcgacgc ggaacgccgc atccagcagg ctggcggggt agtagtcgga
    74281 agagaggata tccagcagac caagctgcgc cagttcactg gccgccacat tgccggagtg
    74341 cgagccgcca cgcacgatat ttggcgcacc catcagcacg ttcatgccat gcttacgcga
    74401 ggcttccgcc gcttcgaacg tggtgggaaa ttcggcgatc acgctgccaa gttggtggga
    74461 ttcggcaacg tgggcgtgcg tggcgtcatc gtggctggcg agcgcaattt gtcgcgcgtg
    74521 gcacagggcg gcgatggatt cgcgattcgg ctgcgaccag cgtgcggcga gcgccaattg
    74581 ctcttcttcg tactgctgca tctgcgcatc ggtgagggag tatttgccct gataatattc
    74641 gcgatacttc tcgcggttag cgaactggcg ctggcccggt gagtggtcca tcagcgacac
    74701 cagcgtcacc ggttcgcgct gaaccagttt ttcaaacagc ggcagtgtgg tgtgatgcgg
    74761 cagttcgcag cgcaggtgca ggcggtgctc ggcgcggttg acgccgcgtt tttgcgtctc
    74821 ttcgatggcg ttgatcatct tctccagatt ctccagccga tcgccgccgt cgcgcacatc
    74881 accaatcgcc acggcgtcca gtacggtggt gatgccgctt gccaccatca gcgcgtcgtg
    74941 gctgctcatc gccgaatggg cgggccagtc gactttcggg cgcggggtga agaatttatc
    75001 gaggttgtcg gtatgcagct caatcaggcc gggtagtagc cagccacctt cgccgtccat
    75061 cgcttctggc aggcggctct ggctttcggc aaaggcgcgg atttcgccat cctgcacctc
    75121 cagcgaaccg tgcaccacct cgttttccag caccagctta acgttattga taatcatgaa
    75181 gaggctccca ttgggtgcag gcggtcggcg acgtcattac gtacagcttc gtcatggaag
    75241 atgccgacga tggctgcgcc acgggctttg gcttcgtgaa tcagttccac caccgcggcg
    75301 ctgtttttgg cgtccagcga ggcggtaggt tcgtcgagca gcagaatggg gtagtcgacg
    75361 ataaagccgc gggcgatgtt gacgcgctgc tgttcgccgc cggagaaggt cgatggtgcc
    75421 aggtgccaca ggcgttccgg cacgttcagg cgggtgagaa gacgcgcggc tttagcggcg
    75481 caggcttcac gcggaacgcc tgtatcgagc agcggctgca tcaccacttc cagtgctgag
    75541 atacgcggga tgacgcgcag aaactggctc acccagccga tggtagtttt gcggatctcc
    75601 accactttgc gcgctggcgc ggtgaccagg tctacccatt cgtcaccgtg tttgatctgg
    75661 atttgccctt cgtcgggcag atagttggcg tacagcgagc gtagcagagt tgatttgccg
    75721 ctgccggaat ggccgtggag caccacgcat tcacctgcgt tgacggtgag cgaggcgcgt
    75781 tgtaagacgg gcaggcgcac gccgttttgc tggtgcagga tgaaggtttt actgacgttt
    75841 tgtacgttaa tcatttttcg cctcgtggtt cgcatattgg ggagcggtgc gcgcattgcc
    75901 cctcacccta accctctccc cgaaggggcg aggggaccga ttgtgcgcgg tgttgaatat
    75961 tgcgccggtt ttctccctct ccctccggga gagggccggg gtgaggggat cagcgcgcac
    76021 ggtgttgcat tcaattctgc aaaaccgatg acaccagcag ttgtgtatac ggatggtgcg
    76081 ggtcgtcgag cacgcggtcg gttaacccac tctccaccac ttgcccctgc ttcatcacca
    76141 gcaaacggtc cgccagcagg cgggcgacgc ctaaatcgtg ggtgacaatc actaccgcga
    76201 ggttcagctc caccaccagg ccgcgcagca ggtcgagcag gcgtgcctgt accgacacat
    76261 ccagtccgcc ggtcggctcg tccataaaca ccagcttcgg atgcgtcacc aggttgcggg
    76321 cgatctgcaa acgttgctgc atgccgccgg aaaaggtggt cggcaggtcg tcgatccggt
    76381 tggcgggaat ttccacctct tccagccact tctgcgcggt ggcgcgaatg tcgccgtaat
    76441 gacgcgcccc ggtcgccatc agccgctcgc cgatattgcc gcctgccgag acctggcggc
    76501 gcaggccgtc gagcggatgc tgatgcacca cgccccattc ggtgcgcagc aggcgacggc
    76561 ggtcggcctc gctcattgcg tacagcgaac ggctctcata gtgaatctcc ccctgctgcg
    76621 gcgtcaggcg cgcggagatt gatttcagca gcgtggtctt cccggagccg gactccccga
    76681 caatgcccag cacttccccc ggccataaat caaaagagac atcgctaaag cctttgcccg
    76741 gcgcgtaaag gtgggtcagg ttattgaccg aaagtaacgg ttgattcatt ggttttttgc
    76801 ctcgctctgt tggcggcaat aatcggtatc ggagcagaca aacatgcggt ttccggcgtc
    76861 atccagcacc acttcatcaa gatagctgtg ggtcgatccg cagatggcgc atggctcatc
    76921 ccactgctga acggtgaacg ggtgatcgtc gaaatcgaga ctttccacgc gggtaaacgg
    76981 cggcaccgca tagatgcgct tctcgcgccc cgcgccgaac agttgcaggg cgggcatcat
    77041 gtccattttt gggttatcga atttcgggat cggcgacggg tccattacgt agcgcccgtt
    77101 caccttcacc ggataggcgt aggtagtggc gatatgaccg aagcgggcga tatcttcata
    77161 cagtttcacc tgcatcacgc cgtactcttc cagcgcgtgc atggtgcggg tttccgtttc
    77221 gcgcggctcg ataaagcgca gcggttccgg gattggcacc tggaagataa tgatctgatc
    77281 ttcggtcagc ggcgtttcgg ggatgcggtg acgcgtctgg ataagcgtcg catcgtccgt
    77341 acgttcagtg gtgtttaccc cggtcacgcg cttaaagaag ttgcgaatcg acacggcgtt
    77401 ggtggtgtca tccgcaccct ggtcaatcac cttcagcacg tcgctttcgc caatcacgct
    77461 ggcggtgagc tgtatgccgc cggttcccca gccgtatggc atcggcatct cgcgcccgcc
    77521 aaacggcacc tgataaccgg ggatcgccac cgcttttaag atagcgcggc ggatcatgcg
    77581 tttggtctgc tcgtcgaggt aggcaaagtt gtagccgctc agattagcca tggttcttct
    77641 cctgttgcag acgtttgagt agctccagtt cggcctggaa atcgacgtag tgggggagtt
    77701 tgaggtgcga gacaaagcct gcggcttcga cgttgtcggc atgtgccagc acaaactctt
    77761 catcctgcgc cgggcctgtc gcgtgctcgc cgtattccgg agcctgcaac gcacgatcga
    77821 ccagcgccat tgccatcgct ttgcgctcgc tcatgccgaa taccagcccg tagccgcgcg
    77881 tgaagtgcgg cggctcatcc ggcgggtcga taaaaccgtt gaccatttca cactcggtca
    77941 tcagtagttc gccgacgttt accgcaaatc ccagctcttc cggcacaatc gacacgtcga
    78001 tgtaaccact gcggatctcg cccgcgaacg ggtgattgcg cccgtaacca cgctgggtgg
    78061 agtaggccag cgccagcaaa tagccttcgt ccccgcgcat taattgttgc aggcgggagg
    78121 agcgtgagca ggggtaaacc ggcggcgtgc gggtgatgtc atccggctgt gcgccgctat
    78181 cttcttcaaa cttcgccagc ccctgacgcg ccagcaggct gaaaacgtgc ggcgacggct
    78241 gttgttcgct gtcggcggtg gtcagcgtcg gcgcttcgcc gtttgccagc agggtgaaat
    78301 cgagcaggcg atgggtgtag tcgtaggttg ggccaagcaa ctgaccgccg ggaatatctt
    78361 tataaaccgc tgaaatacgc cgttcgagac gcatctccgt ggtgtcgagc ggctcgctta
    78421 ccgccagctt cgccagcgtg gtgcggtagg cgcgtagcag gaaaatcgct tcgacgttat
    78481 cgccgctggc ctgtttcagc gccagcgccg ccagttcgcg gtcggcaatg ccgccttcgg
    78541 tcatcacgcg atctactgca agattaagct gctgttcaat ctgggcgacg ctcagttcag
    78601 gcaaatcggt attgccccgg cgtcggctct cttgcagggc gtgggcggcg tcgatcgcct
    78661 tctcgccccc tttcacggca acgtacatca gcacacctcc acatgagtgg ttcgcgggat
    78721 agccagcagc cgctcgccgc aggtcaggat caggtcgatg ccgagcggga acgggtgcgg
    78781 gcgctcggtg agttcgtgca gaatgcactc cggcagctgc ggagcgatca ttcgttcttc
    78841 ggcaataccc gcgccagtaa ggcgcagcat gcgcccgccg ctcaggctgg cgacctgtaa
    78901 aatcagcgtt gcacccgctt ccggcgcaac ggcggtgccg gtggaaaggg cgttaagctg
    78961 ttcgctggaa atcgcctcat ccgtcaccgc gaaggtcgcc tgttccggct ggctgaccag
    79021 cggcgcgttg gtatgaaaac gcaggctctg gctgacgata tcgttactca atggcgcggc
    79081 aagccacacc ggcgtgtcgt tatcggccag cgtcagcagc acgctggtgg tggcgatatt
    79141 cagcggctgc cagccgcgct tgagctgatg cagggcgaca atcacgcccg gctcgctcat
    79201 ggcctttaac aggcgacgaa aactgtgctg ggcatcctgc acgggaagca taaaagcggt
    79261 ttccagggtc atgcgttgtc tccgcgaacc atcgtaaaga agtcgacccg gctggcgttc
    79321 acttcggcct ggcgtgcggc aatgcgtgcc atacggtcag cgtccagcgg ggcaataagg
    79381 gtttctgata agttttgaaa atagcgagat tgctgcatca gcgcgtcaat cagcgcgcag
    79441 cgttcggcgt gctgtttgtc gcgccccagc acccagctgt agccgagcgt gccgtcagtc
    79501 aggcgtacgg cggcgcgggt gagcgtggcg tcgccggcaa aaaagcgttc gccggtgccg
    79561 cccatccgcg cctgaatctg caccaggccg gtttcggcgg cgcggataac ctcatagtcg
    79621 gcggtgatgt tcagtgcttt caggcgggct gccagttcag ccggttggct gtgcgccagt
    79681 acggacatcc agtgctggcg ggtcgcggta tctgcgtgca ttcagtgctc catagtgaat
    79741 tcaatcatgt cggcgcgcgt caggctgacg gagtactccg ccgggctgct ttcaccgtca
    79801 cggtggttaa gggtgcgcac gcacagcagc ggcgacatat tcggaatttc aagacgctgg
    79861 cactctttgg cctgggcgcg acgggcgctg atccgcgtct ggctgcggcg cagcgcaatt
    79921 ccggtttgct cgcgcagaaa atcgtgcagc gaaccgctgt cgaagcgttg cagcgtcggc
    79981 caaagggtga ggtccgcaaa gtagtggtcg attaaacaga gcgcgacgcc gttgacgcga
    80041 cgcagggtgc gcaaatggat aacgttctcg ccctcggtaa tccccagcgc gtcagcgacg
    80101 tgaccggacg cggggcgcaa taccgacagc agtttttcgc tggtgggatg gctaccctgg
    80161 tccagcaggt tctggctaaa acgcgcctgg gcgttgagcg ggtaatcgaa cgggcgcatc
    80221 agcaccagca cgccgacgcc ctgacggcgc tgtacccagc ctttttccac cagttggtcg
    80281 atggcgcggc gcagggtgtg gcggttcacc tcaaagcgcg ccgccagttg ctgctcggcg
    80341 ggaagatagt cgccgcagcg gtagtgttga cgaagctcct gctcaagttt tgcggctatc
    80401 tcctgatagc gtgttgggta gctggtcgga tgtgtagaca agtgcataga tatcaatgcc
    80461 tcgcttatca gataaagtgc ttacgcaacc gttgagagag gaaatccagc aggctgaccg
    80521 tgacgatgat aagcaccatc agggcgcagg tttgctggaa ctggaaaccg cgaatcgctt
    80581 cccacagggt gacgccgatc ccgcctgcac cgaccatgcc gacgacggtc gccgagcgga
    80641 cgttggattc gaagcgatag agggagtagg agatcagcag cggcattacc tgtggcagca
    80701 cgccgtaaag gatctcttcc agcttgttgg cgccggtggc gcgaatgcct tccaccgggc
    80761 cgggttcaat cgcttccacc gcttcggaaa gcagtttcga gagcacgccg gtggtgtgaa
    80821 taaatagcgc cagcacgcca gcgaacggtc cgagaccgac ggcgaccacg aacagcatgg
    80881 cgaagaccat ttcgttaatg gcgcggcagg cgtccatcag gcggcgaacg ggctggtaaa
    80941 cccaccacgg caccaggttt tcggcgctca tcaggccgaa ggggatggag agaaccactg
    81001 ccagcgcggt gccccagacg gcgatttgca gcgtgacggc catttcggtg aggtaatcct
    81061 gccactggct gaaatcgggc gggaagaagt cggcagcgaa ggttgccatg ttgccgccgt
    81121 ctttaatcag cgtaagcggg gccatttccg cgccctgcca cgagacgacc agcacggcga
    81181 gaacaacggc ccagctcaga agcgagaacc agctacgctt ggatggggcg atggtgatgg
    81241 tttgcatgtt tggctccgtt taatgttttc tccctctccc tcaagggaga ggggaccgat
    81301 cgagcacatt actgcaccgc tttactcacc gaactcatcg cgcttaacgc gttgttcagg
    81361 cggtccaggt catccagctg cgcctgaatc tcggtggttt tcgccagctt gtcctgctca
    81421 ttcagtcctt tattgctttt tacgccctgc atctctttaa acagtgcgag ctggcgaatc
    81481 ggcaccagtt gcaggtcgct ggaggcgcgg aacggggccc agcccaggcg ttccagcacc
    81541 gctttctctt ccggcgtttt tccgtagttc ataaagaagt cgtagatctt gtctttggtg
    81601 gtttcggaga gattcttgcg ccagacgatc ggatcgcctg ggatcagcgg cgacttccag
    81661 atcatcttca gttctttcag cttctctggc gcggaggttt tcagcttgtc gaggttttcg
    81721 gtgttgttgg tggcaacatc cacctgcttg ttggcgacgg ccagcgcgtt ggtttcatgc
    81781 ccagcgttga cggtgcgctt aaagtcgctg gcggagatat tgtttttggc aaagacgtag
    81841 taaccgggga cgaggaagcc agaggtggag ttaggatcgc cattgccgaa ggtgagatct
    81901 ttccgcttcg ccagcagatc gttcaggttg ttgatcggac tatctttgtt gacgatcaac
    81961 acgctccagt aacctggcga tccatccgcc gcgaccgtct gggcgaagac ctggccgttg
    82021 gcgcgatcca ccgcttccat tgccgacagg ttgccgtacc aggcgatatc cactttattg
    82081 aagcgcattc cctggataat gcctgcgtag tctggggcaa agaaggcgtt caccttcacg
    82141 cccagcttct tctccatatc ctgtaagaat ggcgtccatt gcggtttcag gttttgctgt
    82201 gattccgttg aaataatgcc gaaattcagc gccttttcct gctcttcggc gtgtgccggg
    82261 cttaacaggg tgctgaggct gaacatgctg gtgaaggcca gcgaggcaat tatcttagcg
    82321 ttcattcgtt ttcctcaatg atggggatgt caggcagctt tcgcgttctc ttcgatgcga
    82381 ttaatgctgc ggtagagatg gtcaaaacgt tcgttatcaa actgttggct gctgccgtcg
    82441 tagaaaacgt gcccctggcg cagggcgacg atgcgttcgc agtagcgcag ggcgtaatcc
    82501 acctgatgca gcgtgacgac cacggtgatg ccgtcgttct gattgatgtc gcgcagggtg
    82561 tccatcacga tgcgggcgga ttccgggtcc agcgaggcga tgggttcatc ggccagaatc
    82621 accttcgcct gctgcatcag cgcgcgggca atcgccacac gctgctgctg tccgccggag
    82681 agggtggaaa cgcgttgatg ggcaaaatgc accatgccaa cgcgggtcag cgcctgtaac
    82741 gcgcgttgtt tctgctcgcg ggtaaaccag ctaaaacagg tgcgccagaa cggcgtgctg
    82801 ccgagcgcgc caatcagcac gttctccagt acgctcaggc ggttcaccag gttgaattgt
    82861 tggaagatgt agccggtgtt ggcgcggctt ttgcggatat cgcgcgccag acggccttcg
    82921 cgctggactg tgcggcccag cagctcgata tggctgccgg cggatttatc gccggtaatc
    82981 aaaccgctta agtgacgtaa aagggtggat ttgctggaac ccgacggccc aagcagagcc
    83041 accatttcac cgtgatgaat gttcagatca accgcatgca gcgcctgatg ctgattgaag
    83101 gttttggcga gcttctcgac acggataatc gtttgcatga tgcagcctcc ctaaaaaagt
    83161 ggctccatcg tggcggatta ttgtgacgat ttggttaatt aaaagtgact aacagatgaa
    83221 gagttaacgg gaattcgatg acagtgcggg agggcgggcc tcccggcgga ttattgcgtt
    83281 ggttgttgtt tgacgacatt aatcatccac ggtacgccaa atttatcggt gactttgcca
    83341 aagccatgcg cccagaaagt ttcctgccag gccatttcga tttttccgtt agcggcaaga
    83401 ttgtcaaacc agcgttttcc ttcttcgacc tgttgcgaat cgagcaccag cgtaaagccg
    83461 gagtagctgg cttttcctga cggcatggca tcgctcatca tgatgtcgct tccggcaatg
    83521 cgcacgttgg catgagcgat ggcggtatcg ggaaattgca ttccggaagg gcagttctcg
    83581 gcgctgtcct gcgctgattt tggcatttcg ccgaagctga ttttatagag cagttcagcg
    83641 cccaacgtac gttgataata agcaattgcg tcggcacagt taccggcaaa agagaggtag
    83701 ggacttaacg gcatgatggt gacctcagtt aagagaagcc agttaagtgt agttcgaaat
    83761 ttatacagat gagagggaag cctgataaga cgcggtcagc gtcgcatcag gcagtcggca
    83821 tcgttgccgg atgcggcgcg agcgccttat ccggcttacc gatcacaata caatcagttc
    83881 tttttcacaa actcagattt cagtttcatc ggaccaaaac cgtcgatttt gcaatcgatg
    83941 ttatggtcgc cttcaaccag gcggatgttt ttcactttgg tgccaatttt cagcatcgaa
    84001 gagctacctt tcaccttcag atctttaatg atggtaacgc tgtcgccgtc agccagcaga
    84061 ttgccgttag catctttaac gatcagctcg tcgctttcct gtgcaggttc tgcgtcgttc
    84121 cattcgtagg cacattccgg gcagatgtac atgccgttat cttcgtaagt gtattcggag
    84181 ttgcattttg ggcagtgtgg taatgacatg tggatttcct caaaagtcag cagggcaaaa
    84241 gcgccaaaaa acggcagatt gccgaaaaag gccgcaatta tacacaaaat ccctgagttt
    84301 gtcgggggta ctgctgattt tagacgacga tcgttttcat ttgaaaggaa acgcaaaaag
    84361 aaaataatct ttttgcctct atttattatt taagataatg ttaatgctct acttgttctt
    84421 ttttttattc acatgatatt tttatctttc tgacgtttta aacgtatcag aagacgttat
    84481 ttattctgat aaaaaacaac acactaaata taaaattatt tcttgcagta attataaaag
    84541 ccgaaaacag aactcaaaaa caatctggct aaataaaagt aacaaaattt gctttaagga
    84601 agaattttct atgtacacac agaccctgta tgagttaagt caggaggctg aacgcctgtt
    84661 acagctttct cgccaacagt tgcagttact ggaaaaaatg cctctctctg tacccggaga
    84721 cgacgcgcca caactggctt taccctggag tcagcctaat atcgccgaac gtcacgcgat
    84781 gctgaataat gagttgcgta aaatttcccg actggaaatg gtgctggcaa ttgtcggtac
    84841 catgaaagca gggaaatcaa ccaccattaa tgccattgtt ggtacggagg ttctgcctaa
    84901 tcgtaatcgc ccaatgactg cgctgccgac gcttattcgc catacgcccg ggcaaaagga
    84961 accggtactg catttttcac atgtcgcgcc aatcgattgt ttaattcaac aattacaaca
    85021 gcgcctgcgt gattgcgata ttaagcatct gaccgatgtg ctggaaatag ataaagatat
    85081 gcgtgcgctt atgcagcgga tcgaaaatgg cgtcgctttc gaaaaatatt atctgggtgc
    85141 ccagcctatt tttcattgtc tgaaaagttt gaatgattta gtgcgactgg cgaaggcgct
    85201 ggacgtcgat tttccttttt ctgcttacgc cgccattgag catattcccg tgattgaagt
    85261 tgagtttgtc catctggcgg ggctggagag ttatcccggt cagttgacgt tactggatac
    85321 ccccgggcca aatgaagccg ggcaaccgca tctgcaaaaa atgcttaacc agcagctggc
    85381 acgcgcctcg gcggtactgg cggtgctgga ttatacgcaa ctgaaatcga tctccgatga
    85441 agaggtccgt gaggcgattt tggcggtggg gcaatcggtg ccgctgtatg tgctggtcaa
    85501 taagttcgat caacaggatc gtaacagtga cgacgccgac caggtgcggg cactgatttc
    85561 cgggacgctg atgaaaggct gtattacgcc acagcagata tttccggtgt cgtcgatgtg
    85621 gggctacctg gcgaatcggg cgcgccatga gttagtcaac aacggtaagt taccagcgcc
    85681 agagcaacaa cgctgggtgg aagattttgc ccatgccgcg ctcggcaggc gctggcgtca
    85741 tgccgatctg gcggacctcg aacatattcg tcatgctgcc gatcagttgt gggaagattc
    85801 gctgttcgcc cagccaattc aggcgttgct tcatgccgct tacgctaacg cctcgttgta
    85861 tgctttgcga tctgctgcgc ataaactgtt gaattacgcg cagcaggcgc gggaatacct
    85921 ggattttcgt gcgcacgggt taaacgtcgc ttgtgaacaa ttgcggcaaa atatccatca
    85981 ggtcgaagaa agtttgcagc tattgcaact caatcaggcg caggtgagcg gcgagattaa
    86041 acatgaaatc gagctggccc tgacctccgc caaccacttt ctgcgtcaac aacaagatgc
    86101 gctgaatgcg cagttagccg ccttgtttca ggatgattcg gagccgttaa gcgagatgcg
    86161 tacccgctgt gagacactgt tacagacggc gcagaacacc atcagtcgcg actttacgct
    86221 gcgttttgcc gagctggaat ccaccctttg ccgagtgtta accgatgtta ttcgccccat
    86281 tgagcaacaa gtcaaaatgg aattgagcga gtcagggttt cgtcctgggt ttcattttcc
    86341 tgtttttcac ggcgtagttc cccacttcaa cactcgccag ctgttcagtg cagtcatttc
    86401 gcgccaggat gcaacggacg agcagagcac gcgtttaggc gttgtgcgtg agactttttc
    86461 gcgctggttg aatcagcccg actggggacg gggaaatgag aaatccccga cagaaacggt
    86521 tgattacagt gtgttgcaac gagcattaag cgcagaagtc gatctttatt gccaacaaat
    86581 ggctaaagtt ctggcagagc aggtcgatga atctgttacg gcaggcatga atactttttt
    86641 cgctgagttc gcttcatgtt tgacggaatt acagacgcgt ttacgcgaaa gcctggctct
    86701 gcgtcaacaa aatgaatcgg tggtcaggct gatgcagcag caattgcagc agactgtgat
    86761 gactcacggc tggatttaca ccgacgccca gctgttacgc gatgatattc aaacactttt
    86821 cacggcagaa cgatattgac caagacgtta ctggacggcc ccggtcgcgt gctggagtcg
    86881 gtttatcccc gctttttagt ggatctggcg cagggcgatg atgcccgcct tccacaagcc
    86941 catcagcagc agtttcgtga acgactgatg caggaacttc tttcgcgtgt gcagcttcag
    87001 acatggacga acggcggcat gttaaatgcg ccgcttagcc tgcgactgac aatggtggaa
    87061 aaactggcgt cgatgctgga tcccggtcat ctggcgctga cgcagatcgc gcagcatctg
    87121 gcgctgctgc aaaaaatgga tcaccgccag cactctgctt tcccggagct cccccagcaa
    87181 attgccgcct tgtatgagtg gttttcagcc cgttgtcgct ggaaggaaaa ggcattaacg
    87241 caacgaggcc tactggtgca ggcgggtgaa cagagcgagc aaatttttac ccgctggcgt
    87301 gctggggcgt ataacgcctg gtcgttgcct gggcgctgtt ttatcgttct cgaagagttg
    87361 cgttgggggg tatttggcga tgcctgtcgt ctgggaagcc cgcaagcggt ggcgttgttg
    87421 ctgggtgatt tgcgcgtgaa agcgacacaa catctggcag agagtatcaa tgcggccccg
    87481 accacgcgtc actattacca tcagtggttt gcctcttcga ccgttccgac gggcggggat
    87541 catgctgatt ttttaagttg gctgggaaag tggaccacgg cagataaaca acccgtttgc
    87601 tggtcagtga cccaacgctg gcaaactgtc gcgctgggga tgccacgact ctgttcagcg
    87661 cagcgtctgg cgggggcaat gctcgaggaa atcttctctg taaatttggc gtaaataatc
    87721 agttacatca atgagtccta aacgaaatcc atgtgtgaag ttgatcacaa atttaaacac
    87781 tggtagggta aaaaggtcat taactgccca attcaggcgt caactggttt gattgtacat
    87841 tccttaaccg gagggtgtaa gcaaacccgc tacgcttgtt acagagattg catcctgcaa
    87901 ttcccgctcc ccttttgcgg ccgtcgcgct gatttttctg gcgtttgcgg aaatgggcca
    87961 actctgcgag gaaagctatg ctgaaaagga aaaaagtaaa accaattacc cttcgtgatg
    88021 tcaccattat tgatgacggt aaactgcgta aagccattac cgcagcatca ctgggtaatg
    88081 caatggaatg gtttgatttt ggtgtttatg gttttgttgc ttacgcatta ggtaaagttt
    88141 ttttcccggg ggctgacccc agcgtgcaga tggttgctgc acttgccact ttctccgttc
    88201 cctttctgat tcgaccgctt ggcgggctct tctttggtat gttgggcgat aaatatggtc
    88261 gccagaagat cctcgctatc actattgtga ttatgtcgat cagtacgttc tgtattggct
    88321 taataccgtc ctacgacacg attggtattt gggcaccgat tctgctgttg atctgtaaga
    88381 tggcacaagg tttctcggtc ggcggtgaat ataccggggc gtcgatattt gttgcggaat
    88441 actcacctga ccgtaaacgt ggctttatgg gcagctggct ggacttcggt tctattgccg
    88501 ggtttgtgct gggtgctggc gtagtggtgt taatttcgac cattgtcggc gaagcgaact
    88561 tcctcgactg gggctggcgt attccgttct ttattgctct gccgttaggg attatcgggc
    88621 tttacctgcg ccatgcgctg gaagaaactc cggcgttcca gcagcatgtc gataaactgg
    88681 aacagggcga ccgcgaaggt ttgcaggatg gcccgaaagt ctcgtttaaa gagattgcca
    88741 ccaaacactg gcgcagcctg ttgacatgta ttggtctggt aattgccacc aacgtgactt
    88801 actacatgtt gctgacctat atgccgagtt atttgtcgca taacctgcat tactccgaag
    88861 accacggggt gctgattatt atcgccatta tgatcggtat gctgtttgtc cagccggtga
    88921 tgggcttgct gagtgaccgt tttggccgtc gtccgtttgt gctacttggt agtgttgccc
    88981 tgtttgtgtt ggcgatcccg gcgtttattc tgattaacag taacgtcatc ggcctgattt
    89041 ttgccgggtt actgatgctg gcggtgatcc ttaactgctt tacgggcgtt atggcttcta
    89101 ccttgccagc gatgttcccg acgcatatcc gttacagcgc gctggcggcg gcatttaata
    89161 tttcggtgct ggttgccggt ctgacgccaa cactggcggc ctggctggtc gaaagctcgc
    89221 agaatctgat gatgcctgcc tattacctga tggtagtggc ggtgattggt ttaatcaccg
    89281 gcgtaaccat gaaagagacg gcaaatcgtc cgttgaaagg tgcgacaccg gcggcgtcag
    89341 atatacagga agcgaaggaa attctcgtcg agcattacga taatatcgag cagaaaatcg
    89401 ataatattga ccacgagatt gccgatttgc aggcgaaacg tacccgcctg gtgcagcaac
    89461 atccgcgaat tgatgaataa gctgaaacgg atggcctgat gtgacgctgt cttatcaggc
    89521 caattgaact cttaaggttc acttaatctc tgacgcgcat actctcctcc aggttaacgg
    89581 aggagagtgc aatgaaaaac cgtgtttatg aaagtttaac taccgtgttc agcgtgctgg
    89641 tggtcagcag ctttctttat atctggtttg ccacgttctg atctttcttc agccgtaccc
    89701 aggcccgcgt gccggaagtc tcttgccggt tttgcaggaa aaactgcccg tgatgcaact
    89761 gtgtaatgcg gctgacaata cttaacccca gaccaatccc gccataacgg ctgtccatac
    89821 gtacaaacgc tttactcaac tccccgcatt tactctcatc aatacctggt ccttcatctt
    89881 caactgccat gaccgctccg ccatcttctt gcagcttaat cataatgttg ctgccttgcg
    89941 ggctgtaacg atgggcgttt tctaccaggt ttcgcaataa catccgcagc agggttgcat
    90001 caccctgaac ggtgatgtcg gcggcgctct ctggcaatag cagggtttgc tgtcgctggt
    90061 cgagcatggt actgagttcg tcatacgagg ggagaatgac atcttccagc agttttacat
    90121 gttgataatt accggaagaa aatgactgtc cggcacgcgc cagttgcagc agctgggaga
    90181 cgctctccat catctgatca agccgtgcca ctaacggtgc tacatcaatg tgatgcgttt
    90241 tcgccagcag ttccagatgc aaacgcaccc ccgccagtgg cgttcgcagt tcgtgcgcga
    90301 cgtcagcggt aaacaacctt tcgttatcca gcgtgctggt caggcgactg accagatcgt
    90361 tcaacgccga aaccaccgct tcgatttcga gggtggcgct gtgaatggca atgggcgtta
    90421 agttgtcggc ggtgcgcgct tccagctctt tttgcagctc cgccagcggg cgggtgatgc
    90481 ggcgtaccgc ctgatagcag ataaatagcg tcaggctgac cataaagacg ccggggacaa
    90541 tcaggctggc gaccgcctcg cggatctcac gcatgatgtg gcgatcgttg ttgcgattgt
    90601 cgcgtagcgc ctgctcaaac agctgaatct gctcggtact ttcatgccat agccagaaga
    90661 cgctgatcag ctcaaacacc aacaaaatgg ccccgatggt caatatcagc cgttggcgca
    90721 gcgatattgg tcggcgcaga aaatgcatca gattcaatta gttttcctca ttcgcgacca
    90781 gcatatagcc aaagccgcgc acggtgcgga tacgggcttt gcccactttg tcgcgcagat
    90841 tgtggatatg cacttccagg gtgttggtcg agggttcatt gtcccagtta tagatgtcgt
    90901 tgtagagaat ttcccgatgc accggactgc ctgctttgag cattaaccgt gacagcagag
    90961 catattcttt gggcgtcaga atcaactctt caccgctcat ccatacctga cggcgaccca
    91021 tattcagcgt cagattgcca acaatcagct cactttcgcc ctgattatta tggcgtcgta
    91081 gcagggcgcg gatacgggcg tgtaactctt ccagcgcaaa aggcttcacc agatagtcat
    91141 cggcaccgac atccagcccg gcgattttgt cggtcagcgt atcgcgagcg gtgaggatca
    91201 gtactggcag ggtgtatttt ttctgccgga tacgggcgag aaaatgcagt ccatcttcgt
    91261 cgggtaaccc taaatccagt accaccaggc tgtaatgacc ggcctcaagg ctttgttccg
    91321 ccatccgcgc ggttgtcacg ccatcgcacg cgtagccttc ggtttgcgcc gccagaatca
    91381 atccctgcaa taacagcgta tcgtcttcaa caatcagaat tttcattcac tcactctcct
    91441 gcaagtttgc agaatatcat ccgcagcctg gtaatacttc gtctcaacgc cagttaatcc
    91501 caatagcgtg gagaataaat tgtcttgtga atagtgttgc gtttgcgcct gtttttgcag
    91561 gcagttctgg tcaacctgat accgtttttg ataatcctcc gacagccaca gcagcattgg
    91621 cacctgtttt tggctatccg gggcgatggc ataaggcaga ccgtgcagat agatgccatt
    91681 ttcacctaac gattcaccgt ggtcagaaag ataaaccagg ctggtggtaa atttatcctg
    91741 atgttctttc agcagattaa tcgctttatc aacaatatag tcgacgtaaa ccagcgtgtt
    91801 gtcgtaagtg ttcaccagtt gctctttgct acaggtctgg atctcattgg tgtcgcaggt
    91861 tggggtaaat ttcctgaact gcggcggata gcggttgtaa taggtcggac cgtggctgcc
    91921 gatggtgtgt aagacaatca cgccatcacc ctgcaggtta ttgatgtact cttccagccc
    91981 gtggaacagc acttcgtcat agcattcgcc gttgatgcac tgaccaggca gattcagcgc
    92041 ggtgacgttc tggtgaggca cgcggtcgca ggcaccttta cagccgccat cgttgtcatt
    92101 ccacagcacg ttgatgcccg ctcgctgaat gatatccagc acgccttcct ggtgctgtgc
    92161 cagctcttct ttgtaatgct cacgcggcat atccgagaac atgcacggta ctgaaactgc
    92221 cgttgccgtg ccgcaagatg cggtattagg gaaatagacc acgttatctt tcgccagccg
    92281 cgggttagtt tcacgcgggt agccgttgag ggagaagttc tccgcccgcg aggtttcgcc
    92341 gacaatcagg atggtcaaat ttttacgttt ttcgttctgc attaacgggt tgcggtgggc
    92401 gtcttcacca attcgcacca gcggtagatt tgctagtcgc tgatgggagt accatgacca
    92461 gctggcaaca atgctgttag aggggcttaa ggatttcacc agctctttgt tattgcggaa
    92521 caacgaggcg tagtctttat aaaacagtgc ggcgaccagc aaaatcagta gtacagaaac
    92581 cagaatattg gctccacgga aaagaacact gcgcagacgc gaggtggcag gtttgatttt
    92641 tatccagcag gcaatcagcg cagcaagcac gccgctgaat cccagcgtta ataacatttg
    92701 cggtgtcatc agcgcataac tttctgccgg agtggtatca ataatattgg caatcatcga
    92761 gcggtcgatg acgatgccgt aagtcattat gaaatattgt gcagccgcgc caaccagaat
    92821 aaacaggcag gccagtggtc gattaagcca taagaaagag cttagtgtca ggacaatatt
    92881 aatcacgctg aaagcgacga ccggcatcga caagaaaacc agtacgttat gcagcgaatc
    92941 cagcggcagc gcctgcaaca cctgtttaaa aaaggcaata ttcaggcaga tagagatata
    93001 aaaagcggcc aacaatagcc aggcgagtaa attcaaagag ggtcttttta gtaggcgctt
    93061 caacatcacg gtgtttccat cgaacaaagt gcgcatatgc tcgcaaagca aaattaagcc
    93121 aaccttaagt tcttaaggtt ggcttttatg tttgctggat taaggggaat taatctttgc
    93181 ttattggtgc atctaaggga tacgggtttt tatgtagccg gttgtaattc agcgcataca
    93241 tggcggtgat gaccatcagg gtgacaaatg accacataac ctctttcgct ccggacccca
    93301 ccacggccca gatgcagtag aggaaggcaa tggtagtaac tgccagatat gccgggcgtg
    93361 ctttaccaaa gtgaccgtgt ccgagcagca gtaacgccgc acaggtgtaa agatatggca
    93421 ccagtgtaaa gatgaccgag acggaagaaa ccagaccgaa ctctttggtc gcgtttggtg
    93481 aaatgctgct gagctggaag atggtcatca aaataccgac gataatcagc cccgccactg
    93541 gcgtacccgc tttatttaca cgggcaaaaa ttggtgggaa cagtccgtca tcggcagcgg
    93601 ctttcgccgt ttgacccgcc agcaacgtcc agccgcccag tgaacctaag caacccgcag
    93661 ctgcgcagaa ggaaacaatg gccccggcgg tgtcacccaa cgccatccgt gcggcatcac
    93721 cgaatggcga agcagaaacg cgcagtgcgg cattagggat catccccata atcgcggtgg
    93781 tagaaagtac atagcaaacg gcggcaatca ataccccacc aatggtggcg atagggacat
    93841 tgcgtttcgg gtttttcacc acacctgcgg caacggaggc actttccaca ccgatgaacg
    93901 accacagcgt aacgttaagg gtactttgaa ttgcaccgaa ggtgcccagg ccgctgacgt
    93961 tccatgccgc catataggtt tcaccacgga accagaacca gccaaatacg gcaatcccga
    94021 cgatggggat cagcgccagc acggtggcaa ctgcctgcac acgggtgatc attttcggac
    94081 cgacaatgtt cagcaggacg aagatccaca gcaccacgac gcaggtgatg gttaatacca
    94141 atggatcttt cagaatcggg aagaagtaac ttaaatatcc tacgccaatg accaccatgg
    94201 cgatattgcc gatccagcag gccagccagt agaggacgtt ggtttgataa ccgagaaacg
    94261 ggccaaagca gcggcgggcg tacgcgtaag aaccaccagg acttggatcg aggaacgaca
    94321 ttttggcgta taccatcgag agccccagcg caccgataat cgtcaccaac catccataaa
    94381 tggcaatccc gccagtagag gccaggtttg caggtaacag aaaaacacct gaccccataa
    94441 tattccccga caccatcagg gtgacgggga ttaagcccac tttgtgagca tcagcatccg
    94501 aagacataat taaactcctg cgaaggcgag cttcgtgaca ataaattacg tcatatcata
    94561 cgcctgcatt cgatagttga attattatcg accgggttat cgctgatgcc gttattaata
    94621 tagctcttcg ttaaggttaa aaatcaggcg acagtgcgtt ctctgtgctg actaacataa
    94681 tgcagcggcg tcataccgta gaagtcttta aagacagaaa taaagtacga cgtactgttg
    94741 tagccgcagg actgtgatac ctgagagata tttttaccgt ccatcattaa ttcatttacg
    94801 gcataacgca tgcggcaggt ggtgattatt tggctataac tggtgttttc gcttttcaac
    94861 tttttcttta acagacttgg gctaagacat aaacaactgg ctaccatact aagattccag
    94921 tctttgtgaa tatcgctttc aataatttga taaacgctgt cacttacaca attacgtaac
    94981 atatacatca ttaatgaaag gaatttttta ctgtcgagaa aacgggatag caccgtgaat
    95041 aataatgcgc gtgttcgttc cttttctgac tcagtttctg cgggcatcat gctgtgttgc
    95101 gcggcaacgc gaaagacgtc tggcgtcagg catggcaggg tgagtatggg cgtagcgcta
    95161 cgttgccata ctggtatttg cgagagatct ttattcagaa accggaggta atctttgatg
    95221 atgtcgtgac taatatgagc aaccaaagtg ttgttaagag aggagacgtc gataatatta
    95281 ttttcacaat ttaataacgc catatggtta gctttaaggc taataggttc tttcccattc
    95341 acccttatcc agacatcttt ttcagtcaat aaaacaatac aaggttggtc gctgcaaatc
    95401 ctcatacatg tactcctgag tgcaaataaa agaccagttc tcgctaaagc aaagcgatac
    95461 cgggaaatgg ttatataatt gtatgatgaa tataaacatg cataaaaata acgagtttta
    95521 ctaatattac aaatttttat agtaatttct catgtacata tttttttaca ggataaataa
    95581 caggcaaaaa aaagcgcggg gtggggccgc gcaaaaagta tgacattttt gtttgcagca
    95641 atttttatga aaacgttgcc atactcaaaa ggcctaaggc caggcggcgt aatgttattt
    95701 aaacaattac gccttcagcg gaatagtggt tacgctttca cgcacataac gtggtaaata
    95761 ccgtcaataa tttcagtccc ttcagtttcg tgttcaaatc cagggaaatg gtggtcccag
    95821 gattgcagcg agcgtaaata acttacttgc ggactgtttt tatcgccgaa gttttcacca
    95881 gacagcagca tcgggattcc tggcggatac gggataactg agtttgccgc gatgcgtcct
    95941 ggcagatttt caatggatac cagttcgaca ttgttgtcga caatcgcgtt gtacgcttca
    96001 cgcggggtga tttccgccat cggcaggccg gaataggctt cgttcaaccg tgcgccaggg
    96061 ttgttttctt tcagccaggc aaacatggtg tcacccagat cgtgaatccc catgttcgcg
    96121 taagtgtcag gatattgttc aacaagttcc ggcatcacct gcgccagcgg tgtgttggcg
    96181 tcatagtggc gtttgaagga gcaaagggtg ttaaccagag tcccccattt cccacgggtt
    96241 acgcccatag agaacaggaa cataatttgg aagtcagtgg tgcgggtagg cacaatgccg
    96301 tggcgaccaa gccaggcagt gaccagcgcc gccggaacac cggtttcttc caattcacca
    96361 tcttcaccca ttcccggagc aaggatgctg actttaattg ggtcgagcat actccagtta
    96421 tccggaatat ctttgaagcc gtgccagctt tcgcccggat gcattaccca gcagtcctga
    96481 acggtggtca gcagtttggt tggtgcgtca gcaaagtcat aggttttgcc ggtttgtggg
    96541 tcggtgacga cttctttgtt ccacggtttg aagaaccagc taccgtcagc ggtgaactct
    96601 ttatatagcc gcgccatcgc ctgacggaaa tcaaccgctt cgtcaatcac ttcctgtgtc
    96661 agtgacaggc cgctgttgcc gtccatcatc gacaccgcca cgtcgttgga tgcgcagatg
    96721 gcatacagcg gggaggtggt ggcatgcatc atgtaggcct ggttgaagcg ggagaagtta
    96781 atcgccccac gaccttcacg tacatgaata taagaagcct gtgacagcgc attcagcagt
    96841 ttgtgggtgg agtgggtggc gaaaacggta ggaccgttgt gatcgccagg ttcgccgcgc
    96901 atggcatagt gatcggcata gatcgggttg aaacgtgcat agccgtacca ggcttcgtca
    96961 aagtgcagac gatcggaggt tttttccagc agatcctgcg cttctttagc gttataacac
    97021 acgccgtcat aggtgcagtt ggtcaccacg cagtaagacg gtttttgccc ggctttgtct
    97081 ttggtcagcg ggctttcact gattttcttc tgcaaggttt caggttgcat ttcctgcgga
    97141 tagattggcc cgataatgcc gtagcggttg cggcttggca ccatatagac cggtttcgcg
    97201 cctgtcagca tcaaaccttg ttcgatggat ttatggcagt tacggtcaac gaccacgaca
    97261 tcgttatcgg tcatgcaagc ctgcatgatg gtgcggttag agccggaagt accgacgact
    97321 accgaccagg agcgatcggc accaaatacg cgtgcggcat atttttcgct ttcgccaaat
    97381 gcgccagtat ggtcaagcaa agaaccgagg gaagttcgtt cgatgcccat gtcggtgcgg
    97441 aacagatttt caccatagta gtcatggtag aaacgtccgg cgggtgtttt ggtaaaacca
    97501 acgccgccct ggtggcctgg cgctgcccag gaatattcat ggatgtcact atatttcatc
    97561 agcgcgctga acagtggcgg caacagctgc tggcggtagc gggtcatcgc ggcaacggcg
    97621 cgtccggcga taaagtcggc ggtatcttcc agaatccagg cgaattcatc gacaagctcc
    97681 agcagatccc ggtccattgc ggcgagggct ttttcccgat cgcccaacag gaagaccggc
    97741 acgttttgtt ggcgctcatg aagcttaccg atcaattgtc tgacgttttg atgttcgtcc
    97801 ggatgttcca tttgatagct gaacatcagg cagtcaatgg cttcgtttga agagagaatg
    97861 gcaaaaccat catcaaagga ggtggattta atcacggtaa cattttgctg gcttaaagca
    97921 tctgccagac gctcaacggc gttaccgacc caggtgtctt gatggagaaa ctcgctttca
    97981 acaattaata ctttcatcat tgcttacccg gttatgaagg aatcttcgtt gcgggcaagt
    98041 atcttccggc cttttcgggc tgtaaagcgc gtgaaaaaca aacatttttg aaatatttag
    98101 ttttataaac aatacgttat gtgattattt taaattattt tcactgctta ttatcgacgg
    98161 ctaaactatt tttttggctg atactgatat cgtctttagc cgggaaacgt ctggcggcgc
    98221 tgttggctaa gtttgcggta ttgttgcggc gacatgccga catatttgcc gaacgtgctg
    98281 taaaaacgac tacttgaacg aaagcctgcc gtcagggcaa tatcgagaat acttttatcg
    98341 gtatcgctca gtaacgcgcg aacgtggttg atgcgcatcg cggtaatgta ctgtttcatc
    98401 gtcaattgca tgacccgctg gaatatcccc attgcatagt tggcgttaag tttgacgtgc
    98461 tcagccacat cgttgatggt cagcgcctga tcatagtttt cggcaataaa gcccagcatc
    98521 tggctaacat aaaattgcgc atggcgcgag acgctgtttt tgtgtgtgcg cgaggtttta
    98581 ttgaccagaa tcggttccca gccagagagg ctaaatcgct tgagcatcag gccaatttca
    98641 tcaatggcga gctggcgaat ttgctcgttc ggactgttta attcctgctg ccagcggcgc
    98701 acttcaaacg ggctaagttg ctgtgtggcc agtgatttga tcaccatgcc gtgagtgacg
    98761 tggttaatca ggtctttatc cagcggccag gagagaaaca gatgcatcgg cagattaaaa
    98821 atcgccatgc tctgacaggt tccggtatct gttagttggt gcggtgtaca ggcccagaac
    98881 agcgtgatat gaccctgatt gatattcact ttttcattgt tgatcaggta ttccacatcg
    98941 ccatcgaaag gcacattcac ttcgacctga ccatgccagt ggctggtggg catgatatgc
    99001 ggtgcgcgaa actcaatctc catccgctgg tattccgaat acagcgacag cgggctgcgg
    99061 gtctgttttt cgtcgctgct gcacataaac gtatctgtat tcatggatgg ctctctttcc
    99121 tggaatatca gaattatggc aggagtgagg gaggatgact gcgagtggga gcacggtttt
    99181 caccctcttc ccagagaggc gaggggagcg tccgagcatc atgaggccga aaactctgct
    99241 tttcaggtaa cttattacca taaactcaga tttactgctg cttcacgcag gatctgagtt
    99301 tatgggaatg ctcaacctgg aagccggagg ttttctgcag attcgcctgc catgatgaag
    99361 ttattcaagc aagccaggag atctgcatga tgactgcacc caaaattaca tttatcggcg
    99421 ctggttcgac gattttcgtt aaaaatattc ttggtgatgt gttccatcgc gaggcgctga
    99481 aaacggcgca tattgccctg atggacattg atcccacccg cctggaagag tcgcatattg
    99541 tggtgcgtaa gcttatggat tcagcagggg ccagcggcaa aatcacctgc cacacccaac
    99601 agaaagaagc cttacatgat gccgattttg tcgtggtggc atttcagatt ggcggttatg
    99661 aaccttgcac ggtgactgat ttcgaggtct gtaagcggca tggtctggaa caaaccattg
    99721 ccgatacgtt ggggccgggc ggtattatgc gcgcgctacg taccattccg catctgtggc
    99781 aaatttgcga ggacatgacg gaagtctgcc ccgatgccac catgctcaac tatgttaacc
    99841 caatggcgat gaatacctgg gcgatgtatg cccgctatcc gcatatcaaa caggtcgggc
    99901 tgtgccattc ggtgcaggga acggcggaag agctggcgcg tgacctcaat atcgacccag
    99961 ctacgctgcg ttaccgttgc gcaggtatca accatatggc gttttacctg gagctggagc
   100021 gcaaaaccgc cgacggcagt tatgtgaatc tctacccgga actgctggcg gcttatgaag
   100081 cagggcaggc accgaagccg aatattcatg gcaatactcg ctgccagaat attgtgcgct
   100141 acgaaatgtt caaaaagctg ggctacttcg tcacggaatc gtcagaacat tttgctgagt
   100201 acacaccgtg gtttattaag ccaggtcgtg aggatttgat tgagcgttat aaagtaccgc
   100261 tggatgagta cccgaaacgc tgcgtcgagc agctggcgaa ctggcataaa gagctggagg
   100321 agtataaaaa cgcctcccgg attgatatta aaccgtcacg ggaatatgcc agcacaatca
   100381 tgaacgctat ctggactggc gagccgagtg tgatttacgg caacgtccgt aacgatggtt
   100441 tgattgataa cctgccacaa ggatgttgcg tggaagtagc ctgtctggtt gatgctaatg
   100501 gcattcagcc gaccaaagtc ggtacgctac cttcgcatct ggccgccctg atgcaaacca
   100561 acatcaacgt acagacgctg ctgaccgaag ctattcttac ggaaaatcgc gaccgtgttt
   100621 accacgccgc gatgatggac ccgcatactg ctgccgtgct gggcattgat gaaatatatg
   100681 ctcttgttga cgacctgatt gccgcccacg gcgactggct gccaggctgg ttgcaccgtt
   100741 aaaacgcgac taaacgctac tgcgccgggg gatttattcc ggcgcacacc tctgacgata
   100801 ccaataacag aaggcgggcg ttggtaacag cgacccgata ccctatgagc atttcaatga
   100861 ctacaaaact cagttatgga tttggagcgt tcgggaagga ttttgcgatc ggcattgtgt
   100921 atatgtacct catgtattac tacaccgatg tcgttgggct gtctgtgggt ttggtcggta
   100981 ctttgtttct ggtggcgagg atctgggatg ctattaacga tccgattatg ggatggattg
   101041 taaatgctac gcgatcgcga tggggtaagt tcaaaccctg gatcctgatc ggtacgttgg
   101101 caaactctgt aatcttattt ctcctcttta gtgcgcatct gtttgaaggt actactcaga
   101161 ttgtctttgt ttgcgtgacc tacatcctct ggggcatgac ttacaccatt atggatattc
   101221 ccttctggtc gctggttcca accattacgc tcgataaacg tgagcgcgaa caactggttc
   101281 cttatccgcg tttttttgcc agtctggcag gctttgttac ggcaggtgtg acgctaccat
   101341 ttgttaatta tgtcggcggt ggcgatcggg gatttggctt tcagatgttc actctggtac
   101401 taatcgcctt ttttattgtt tcaaccatca tcactctgcg caatgtgcat gaagtctttt
   101461 cgtcagacaa tcaaccgtct gctgaaggaa gccatctgac acttaaagcc atcgttgcgc
   101521 taatttataa aaacgatcag ctttcatgcc tcttgggtat ggctcttgct tataatgtag
   101581 ccagcaacat tattaccgga tttgctatct attatttctc atatgttatc ggtgatgcgg
   101641 atttgttccc ctattatctg tcgtatgcgg gagctgctaa cctggtgacg ttagtattct
   101701 tcccacgctt agttaaatca ttatcccgac gcattttatg ggccggagca tctattcttc
   101761 cggtgttaag ctgtggtgtt ctcctgttaa tggcattaat gagctatcac aacgtcgtcc
   101821 tcattgtgat tgcgggtatt ttgctgaatg tgggaacggc gcttttctgg gtattacagg
   101881 tcatcatggt ggcagatacc gttgattacg gtgaatataa actgcacgta cgctgtgaaa
   101941 gtatcgctta ctccgtgcag actatggtgg tgaagggcgg ttcagccttt gcggcttttt
   102001 tcattgcggt tgtgttaggg atgattggct atgtaccgaa tgttgaacag tctacgcaag
   102061 ccctattagg tatgcagttt attatgattg ctctaccaac tctgtttttc atggtaacgc
   102121 tgattctcta cttccgtttc tatcgcctca atggcgacac gctgcgcagg atccagattc
   102181 atctgctgga taaatatcgc aaagtaccgc ccgagcctgt tcatgctgat attccggtcg
   102241 gtgcagtgag tgatgtgaaa gcctgacgtg gacaatagct aacgaaaatg gcctgatgcg
   102301 atatgtttat caggccacgt cttaatgtaa tgctttggat ttgcaaattt tgtagaccgg
   102361 ataaggaatt cacgccgcat ccggcatcaa caaagcgcac gttgttatcc ggttatcaag
   102421 ccaaagcgcc gtagctggcg gcaatgaaag cgagcgagga atatcaccgt cgttaatgcg
   102481 ccaagcagcg cgcagaacat atcggattgc gtgtcccact ggtcgccctg ggtgccgaga
   102541 aaatcatccg ctccctggcc cattgccagc gccgcccacc actcgattaa ttcatacatc
   102601 gcgcttatcg ccagcgccac gcagcagacc aggaacgcca ccattttacg tccgcgcacg
   102661 tacatcccgc gcacgagaat ttctcgtgcc accagtgcag gcaccagccc ctggaaaaaa
   102721 tgccccagct tgtcatacgg attacggctc aaccccagcc attcctgcac ctcaaaaccg
   102781 atggggactt tcgcgtaggt gtattgcccg ccgaccatca ggatgatggc gtgaaagaaa
   102841 atgagcgtat agagcagcgg cgttaacgga taacgtttag cggtggcaag cagtagcggc
   102901 acgacaataa tcaccggtgt cacttccatc agccaggtga gtttgtcatg ggccgaaatg
   102961 ccggtataaa tcaggattaa cgttagcgcc agtgcgccgg tgttaagaat taacggcttg
   103021 agtgtgcggg tcatggtgag ttcgcaagtc agggaaaata ctgactattc accagcaagc
   103081 gtgaaattac aatcgcaaaa gaggtggccc agggggatca cctggcagca tgctgccagg
   103141 cgctgggccg aagaggttac ttagtgcagt tcgcgcactg tttgttgacg atctgctgga
   103201 agaagtcgtt acctttgtca tcgaccagga taaacgccgg gaaatcttct acttcgattt
   103261 tccagatagc ttccataccc agctccggat aagcgacgca ttccagatgc ttgatgctct
   103321 gctgcgccag taccgccgcc ggaccgccga tgctaccgag gtagaagccg ccgtgtttat
   103381 gacacgcgtc ggtaacctgc tgactgcggt tacctttcgc cagcatgatc atgctgccgc
   103441 cgtgggattg cagcagatcc acgtaggagt ccatacggcc tgcggtggtt gggccaagtg
   103501 aacctgatgg ataaccggca ggggttttcg ccggacccgc gtagtagatc gggtgatctt
   103561 tgatgtactg cggaagttct ttaccggcgt caatcagctc tttcagcttg gcgtgtgcaa
   103621 tatctcggcc cacgataatg gtgccggtga gcgacaaacg agtggatacc gggtattgcg
   103681 aaagctgggc gaggatctct ttcatcgggc ggttaaggtc aactttcacc gcttcgcctt
   103741 caccggcctg gcgcagttct tgtggaatgt actggcctgg gttgtgttcc agtttttcga
   103801 tccagatacc ttcgcggttg attttcgctt taatgttacg gtcagcggag caggagacgc
   103861 ccatgccgac cgggcaggat gcgccgtgac gtggcagacg gataacgcga atgtcgtgcg
   103921 cgaagtattt accgccaaac tgcgcgccaa gaccgagttt ctgggcctct tccagcagtt
   103981 cctgttccag ctggacatcg cggaacgcct gaccatgttc gttcccttcc gtcggcagtt
   104041 catcgtaata gtgagcgctt gctaacttga ctgttttcag gttggtttcc gcagacgtac
   104101 cgccaatcac aaacgcgata tggtacggcg ggcaggctgc agtaccgagg gtacgcattt
   104161 tctcgacgag gaagtttttc agtttgccgg gagtcagcag ggctttggtt tcctggtaga
   104221 gatacgtttt gttggcagag ccgccgcctt tcgcaacgca aaggaatttg tactcatcgc
   104281 catctaccgc gtacaggtcg atttgcgcag gcaggttagt gccggtgttg acctctttgt
   104341 acatgtccag cgccgcattc tgtgaatagc gcaggttatc ttcgatatag gtgttataga
   104401 cgcctttcga cagcgcttct tcatcaccgc cgccggtcca cacgcgctgg ccttttttac
   104461 cgacgatgat cgcggtgccg gtatcctggc aggtcggcag cacgcctttg gcggcgattt
   104521 cggagtttct taagaattgc agcgccacgt acttgtcgtt ttcgctggct tctggatcgt
   104581 gaagaatagc cgcaacctgt ttctggtgtg ccgggcggag cataaaagaa gcgtcgtgaa
   104641 aggcttgctg cgccagcagg gtcagggctt ctggttccac tttcaggatg gtttcgccgt
   104701 cgaagtcggc aacgctaacg taatcggaag tgagtagata gtattcggta ttgtctttcc
   104761 ccatcgggaa aggtgcctgg tagataaagg gtttgtttga catagcttcc agcctgtaac
   104821 tctgtatttg ttattcgaaa atggcgtgcc gcgtagtcac gggcggcacg caaagtgcat
   104881 ttataagaac ccgtacatcg cggcgaagat ccagccgaag acgcacgata cgctcacacc
   104941 aatcaacccc ggcagaataa agctgtggtt gatgacgaag cgaccgatgt gggtggtgcc
   105001 ggaacggtca aactgaatcg ctgccagatc gctcggataa gtcggcagga tgtaataacc
   105061 gtagcaagcc ggtgctgaag ccacgatgta tgccggatca acgccgatcg ccagcgcgac
   105121 cggaacaatc gccgccagcg ccgcagcctg agagtttaca aacttggaaa ccagcagcag
   105181 aacaatggca taggcccacg gatactcttt caccatttca cccagtacgc cctgaatttc
   105241 agacatatgc gcaccgaaca tggtttctgc catccatgcg ataccgtaca ccgccacgat
   105301 ggcgatcata ccggaacgga agacttcgtt ttttgagata gacgcgggat tggttttggt
   105361 caggataata atcagcgccc cggtcagcag cataaacatc tgaataacca gtaccatcga
   105421 cagcggtttg ccgccgaagg atggacgcag gtccgaatca gcaccaagaa gggcgactac
   105481 agcgattgca ccgaggaaaa tccacattgc cagccagttg cttttcggca gttttttatc
   105541 gagcagcgtc gcggtatcac cgtaaacata ctcacggttt tccggtacgg agatgaattt
   105601 ctggaactct tcgtctttat ccagatcttt accgcggaac cagctgaaga taccgatcgc
   105661 caggataccg attaacgtcg atggaatggt gattgccagc aggtcgagga actcaagatg
   105721 gcgaccatca aaggtgacat tacccagcat tgcaaccaga gacacgaccg caaccgacac
   105781 cggactggcg ataatcccca tctgtgcacc gatagaactt gccgccatcg gacgttccgg
   105841 acggatgttg ttcttaatgg cgacgtcgta gatgatcggc agaatggtgt aaaccacatg
   105901 acccgtaccg caaagaatgg tcagtgtaca ggtcacaaac ggcgcgacaa ttgagacata
   105961 tttcgggttg cggcgcagca acttctcggc aatttgcagc atgacatcaa gaccgcccga
   106021 agcttgcaag gtcgccgatg ccgccaccac cgcaatgata accagcatga catcaactgg
   106081 tggtttacct ggctgaaggt ggaagacgaa gaccagaatg accagaccga taccgcctaa
   106141 taaacccagc gcgataccac cctttctggc accataaaac agacatatca gtattatgat
   106201 aagttggata gtaaataaca tgtgtgaacc ctcgcgataa tcctatttaa atttttgctg
   106261 aatagatcac agtcacgttc tgttttgtat gaactgtttt cagagcctga ctaaatatcc
   106321 gtttggtcgt taccggcttt agcaaatacc tcacagtaaa tattggctga ttgaagcagc
   106381 ggcaaattta tgcactgtga taagcggctt tttcagagag aaaaactctc tggcgatggg
   106441 tttatagttt gtactgcgca gatgttaatg ttttgtttgc tgttatctta ttgatattta
   106501 tcggtctatt ttttgttctc agggcagata tcattaggtt taaagattaa tccgatctac
   106561 ccatttgtgg gtaaaaatac acataatgcg ggtgacataa tagttaatta acttttgtta
   106621 gcgttttgaa attaaaaaca ccgttcacct gaagagatat taatttttag cgatgatgga
   106681 gggataatta tatttgatct ggcacaagtt ttactgatga aggatgtaac ttgtgccagg
   106741 ggtattttgc attacggtaa taattattac ttacagataa ccagcgatcc gttattggca
   106801 atattgtttc agtagtgagt agtgttctgc ctgaatacgg taacggtaaa ccggacgccc
   106861 cgtgacgcca taatggatac tggtgaacaa gatgtggcag ttgaccagcc agatgaggta
   106921 tttacggcag gaaacacgcg agatgttaac ctcgttagcc agctcgtcgg ttgaaaactc
   106981 atagtcctga tgcgcgtcaa tccactggca cagcgtgcgt aacgtctgcg gcgttaagcc
   107041 ttttggcaag cgacgaggat cctgttcgtt ggagctgctg ccgtggatta gctgatcaag
   107101 ctcggcctgg tcataatact gatgtttttc cagcgccatt ttcttttgcc gccagccggt
   107161 gagcgcctct tcaaagcggg aagcctggaa gggtttgatc aggtaatcca cgacaccgta
   107221 atgcagcgaa tctttaatgg ttgccgcatc ggctgcggag gagatgacaa tcacatcact
   107281 tttgcaacgc gcgttatgca ggacaggcag taaatcgagc ccgttctctt tttgcatata
   107341 gatatcgagc aatatcaggt cgataggcgt atcgctattg aagataatct ctttggcttt
   107401 ctccagcgtc gaggctgttc cacagcattg aaagcctggg atttgtgcta cgtatcggcg
   107461 attcagctcc gcgaccattg cgtcgtcatc gataattaat acattgatca tctgttcgac
   107521 ctctccccgt cccagggtat ctggacaaaa aattgtgtga aaatcccggg ttccgattcc
   107581 acggcgatgc tgccgccgag attttctacc tgttgtttga caagtgctaa accgacgcct
   107641 cgctcgcttc cttttgtcga gacacctttg tcaaaaatgt gatcgatttt gtcgggtgcg
   107701 atccccggtc catcatcgtt aacttcgcag tgcagccagc cgtgacggta gtgcaatgtt
   107761 acgctaattt cgccaccggg ttccggccct aatgcttcca gcgcgttttc tatcagattt
   107821 cccaatgtgg taatcagcgt cgcgacctgg tcctcactgc cgctgtctgg cagctggctt
   107881 tcactgttta aaatcagcgt atggcctaaa tcggtcgcgc gattaatctt gctgattaaa
   107941 aaaccagcga taaccggaga tttgatctta cccagcagag agccaatctc ttcctgatag
   108001 ttattggctg ttttgagaat gtaatcttcc aactgcttat aactcttcag atgcaataat
   108061 ccgagaatca catgcaattt attcataaat tcgtgggatc gttcacgaag tgcatcagca
   108121 tagttgacca gaccgtcgag tcgctgcatc agtttacgaa cttcagtttt gtccctgaag
   108181 gttgaaatgg caccgatgat aacgccatta ctgcgcaccg gaacggtgtt gatcagtaat
   108241 agccggtctt taatcgtaat ctcttcgtcg cggcgcggcg taccgtcgcg taacacttcc
   108301 gagacatcta ccacctgtga ccatgagtgg ctgagcgtcg acagtttctc atcgtcctgc
   108361 gacttacggt aattcagcaa ttcttgtgcg gcatcgttga tcagcgtgac ctcgccgcga
   108421 tcgtccacgg caacgacgcc ttctttgata gactgcaaca tggcctggcg ctgctcaaac
   108481 agcgtggaga tttcgtaggg ttccaggccg aaaaggattt ttttcagtac cttaaccaga
   108541 atgcaggtgc caatcagtcc gaccagcatg ccaaataata ccgaccagat aatgctccag
   108601 cgactgtcat tgatttgttg ggtcacgcgg cttaactcaa ggccgatcgc caccacgcca
   108661 atttgtttat gattttcatc gtagatgggg gtaaatacgc gtaaagcctg cgccagaaaa
   108721 ccgcgattga tagcgacatt ttctttgcca ttcagcgctt taaggatgtc atcaccttta
   108781 aatggctgac caatacgctg ggcttcagga tgcgagtagc gaagactttg catatcggta
   108841 acgacaataa acagcagatc gttgcgtttg cgcacggctt ccgcgatggc ctggattccg
   108901 ctctcctgcg gttttttctg caagccctga cggatttccg gcgagtcggc gagggtacgc
   108961 gccactgcca gtgccttgtt ggccagcccg tctcgcgtca tatcactgat ttgcgagaag
   109021 taaatcagat gcaccaccaa tagcaccgag aacagtaccg cgctgaccat taagatcact
   109081 gtggtactca atttcatcgg acgtttgcgt aacatgcggt agggcaatga atgtctcatc
   109141 agcttccttg tgtgacaaat ttcttaagca ttatctctga tgaggcgggt aattcaaagg
   109201 gagtaagaat gattggctat ataggggaag agactctggc aacggaaact gccagtgctg
   109261 tatgaagatt ccagggctat gcttatagcg ataatcatac tgatgagaga gggaaagtca
   109321 tggatcaggc gctactggac gagggttatc gctgttatac cggcgaaaag atcgatgtct
   109381 atttcaacac tgcgatatgt cagcattctg gcaattgcgt acgtggcaac ggcaagttat
   109441 ttaatctcaa acgaaagccg tggatcatgc cggatgaagt cgacgtcgtc actgtggtta
   109501 aagtgattga tacgtgcccg agcggcgcgc tgaaataccg tcataaataa gcgagggtaa
   109561 aatggaaata cgcgaaggcc acaataaatt ttacattaat gatgaacagg gcaagcaaat
   109621 cgctgaaatt gtctttgtgc cgaccggaga gaatttagcg attatcgaac ataccgatgt
   109681 cgatgaaagc ctgaaagggc aagggattgg taaacagctg gttgcgaaag tcgtggaaaa
   109741 aatgcgtcgg gaaaaacgaa aaattatccc attatgtcca tttgcgaaac acgaatttga
   109801 taaaacgcgg gagtatgatg atattcgcag ttgatgggag agtacagatt cacgatattt
   109861 ttcatgctct ctgcggtgtg atgctggaga gcatctgaag ggtaggggga tgcacaaaga
   109921 atgggcagag agcgcgtttt tttgtcccaa gtcatcccct ttactgagca aaaaaaagaa
   109981 tatctcctat atgagaatca tcaatcgggg ttaataagtt ttgcgtcccc agagtgttca
   110041 atattgatag gagtcatatt atggaaggta aaaacaagtt caatacttat gttgtttctt
   110101 ttaattatcc atcatcttat tcctcagtgt tcttaaggtt aagatcattg atgtatgata
   110161 tgaatttctc ctctatcgtg gctgatgaat atgggatacc acgacaattg aatgaaaact
   110221 ccttcgcaat aacgacatcg ttagccgcaa gtgaaatcga agatttaatc aggcttaaat
   110281 gcttagactt accggatatt gactttgacc tcaacattat gacagttgat gactatttcc
   110341 gtcagtttta caagtagcca ggaaggataa gaagaacata aaaaatggca ctattctcta
   110401 aaatattaat tttttatgtg attggtgtga acatatcctt tgtcattatc tggtttatct
   110461 cacatgagaa aacacatatt cgtttactta gtgcattcct ggtcggaata acctggccaa
   110521 tgagtctgcc tgtggcatta cttttttctc tcttttagga gctgtagttg agtggacccg
   110581 ctggttatcg tgaaacgaag cgtctctcct caaaactgga tttgcggaac gatgaacgaa
   110641 gcagtcgcct gactgcttcg ttcattaaag tgaaatttat ttctgtgggc gcatcgccgg
   110701 gaagagaata acgtcacgaa tagtatgact gtttgtaaac agcataatca ttcggtcgat
   110761 gccaataccc agaccagcgg ttggcggcag accatattcc agcgcagtca cgtaatcttc
   110821 gtcatagaac atggcttcgt cgtcacctgc agctttagcg ttaacctgtt cctggaaacg
   110881 ttcagcctga tcttctgcgt cgtttaattc gctaaaacca ttaccgattt cacgaccacc
   110941 gatgaagaat tcaaaacggt cggtgatttc cgggttaaca tcattacggc gcgccagcgg
   111001 ggacacttct gccggatatt ccgtaataaa ggttggctga atcagatgtg cttctgccac
   111061 ttcatcaaag atctccgtga caatacgtcc cagcccccag cttttctcta ccgtaattcc
   111121 gatagattca gctaatgctt tagcagcatc aaaattatcc aggtcggcca tatcggtttc
   111181 tggacgatat tttttgattg cttcgcgcat ggtgagtttt tcaaacggtt tgccgaaatc
   111241 aaacacatgc tcgccataag tgactttagt ggtacccaga acctcttgtg ccagggtgcg
   111301 gaacagtgac tctgtcagtt caatcaaatc gtggtaatcc gcatacgcca tgtagagttc
   111361 catcattgtg aactcaggat tatggcgaac agaaatacct tcattacgga agttacggtt
   111421 gatttcgaat acccgttcaa aaccgcctac aaccagacgt ttcagataca gctccggcgc
   111481 gatacgcaga tacatatcta aatccagagc attatgatgg gtaataaacg ggcgagcaga
   111541 tgccccacct ggaattacct gcatcatcgg ggtttctact tccataaagc cgcgcgcgac
   111601 catgaactgg cggatagcgg ccagaatttt tgaacggaca acaaacgttt gacgggattt
   111661 atcgttagcg atgaggtcca gataacgttg acgataacgg acttcctgat cctgcagacc
   111721 atggaattta tctggtaaag gacgtagtgc ttttgtcagc aggcgcaact cagtacagtg
   111781 aatggaaagc tcaccggttt gcgtcttaaa cagcgtaccg cgggcaccga taatgtcacc
   111841 cagatcccat tttttaaact gatcgttata aacaccttct ggcaggctat ctcttgcaac
   111901 gtacagttga atacggccac cgacatcctg caacgttaca aaggaggctt tccccatgat
   111961 acgacgggtc atcattcggc cagcaaccga gacttcaatg tttaaggatt ccagttcctg
   112021 gttatccttc gcatcaaact cttcgtgcag ctggtcagag gtatggtcgc ggcgaaaatc
   112081 attgggaaac gccacacctt gctgacgtag tgccgccagt ttttcgcggc gatttctcag
   112141 ttcatcgtta aaatcaatag cctcattggc tccccgtgtt tcttgttcag acattttggt
   112201 tcctctaaat ccagctttca atttagcgta gataaagaga cagatcggta cactgtatta
   112261 tctgcctcga ctattaataa ctcagtttat cttatcgaaa ctaacgctta cctcaagttg
   112321 atatcactaa taaataacca tcaaaatggt ttaaatgact tattaatcta gatttgctag
   112381 tgttataaac tgaaagttaa tttattcata accaattgtt tttacgacag ttaatcgttg
   112441 ctctcctgta tcatattcgt cggcgtgcta aacagaaaac gggtagcaaa ggccaatacg
   112501 acgatgatcg cgacacaagc caacgtccac tctcccatct gagaaaagaa tcgctggtaa
   112561 gctgcaattg ccataccgct aatttgcgac tctgtcgtct gctgtgccac gacgcctgcc
   112621 agccagttgg cgaccgcgcc tgttgccagc atataaatac cggttaatac gccagacatt
   112681 ttcagacgcg tgatttgcgc aatcgccacc gggtcaataa agagttcggc aaagcccatt
   112741 agcgccaacc cggatatcat cacgcccatt gacgcttgac cgtcagccgc tgcatgtcgg
   112801 gcatcaaatg ccaacaacat aaagccacaa gccatcagca gtaagccaaa ggcaaacttc
   112861 agccagacgc gcaatgttga gttgccgcgg ctttctggcg acgccagcca ggccagtaca
   112921 accccagcga gcatcaccgc aattgcattc accgactgga atagtgctgt aggtacttca
   112981 atattgaatg cctgacgatt cacaaagcga tcgataaaca agctgatggt actgccgccc
   113041 tgttgtgcca gtacccagaa caatgtcccg acaaacatca acaatacaat ttgccaaaga
   113101 gcacggcggt gttcggggaa tttgaccatc atgcgggcaa tgatttgtgc ggcaatgagg
   113161 caaacgatcg ccagcaaata tccggaccag tcgttctcca gcagcagggt aaaaaatact
   113221 ggggctaaac agagcatcac cactaaccag ctccataccg gtaaggcaaa tttgacgcta
   113281 gtgagcgctt ttttatccat actacgcgtg gactggaaat gacgatgccc gcttaagaaa
   113341 atcaacaaac cgataaacat gccgccaccc gcaagggcaa agccaacatg ccatccatac
   113401 cactgagcag ccaggccgca ggcgattggg gcggcgatag aaccgatatt gcccgcagca
   113461 tacagcagcg aaaaaccgcc atcacgtcga tgatcgttct cgtcgtaaag ctcgccaagc
   113521 aaacagctga tgtttgattt gaataaaccg tagccacaaa taatgattgc cagcgccaga
   113581 tacaggctaa aggttgaatt tgtatcaatg cccagcacca catggccaag ggtcattaac
   113641 agcgcgccgg caatcactgc agtgcggttg ccgagcaggc ggtcggcaag ccagccgccg
   113701 agaatagggg taacgtaaac cagagaagca tatgcgctga acaggctgat ggcatggtta
   113761 tcatcaaaac caagctgatg ggtgagatag agaatgagta aggcacgcat gccgtaaaaa
   113821 ctgaagtact cccagatttg gatcgccacg atatagtata tcgcgcgcgg ctgtgagggt
   113881 gttttcatgt gttctcctta tgagcaaaaa agggaagtgg caagccactt cccttgtacg
   113941 agctaattat tttttgcttt cttctttcaa taccttaacg gtatagcggc catcagcctg
   114001 acggtatgca ccgtgaatat cggtttcaaa gcccggatag tgagcgccga tttcacacag
   114061 catctgcagg aactccagaa ccggacggct ttcttcggtg atcatttcac ccggcattac
   114121 cagaggaact cccggcggat atggaaggat catattggcg ttaatacgac ctaccatttc
   114181 gtcgaggtaa acttcttcgg tcataccgtg cagctctttc tggaacgcag catacggagt
   114241 cattaccatc gtcggcagca cttcaaatgc gcgatacatc agatccggca gattgtggtg
   114301 aacaatcagt ttgtggatat tctgagccag ttcctgaata cgcatgtttt catagaattc
   114361 aggatcttca cgatacagag acggcagcat gtttttcaca cgcaggttca ggtcgaacgc
   114421 acgtttgaag tcagtcagag cacgcagcag gctcagtgct ttggtcttat cgataccgat
   114481 gctgaacagg aacagcaggt tatacggacc ggttttctca acaacgatgc catgttcgtc
   114541 gaggtatttc gccacgatgc tggccggaat accaaagtcg ctcatggtgc cgtctttttc
   114601 catccccgga gtcagcaggg tgactttgat cgggtcaaga tacatgtgct cgttatcgat
   114661 gtttttgaag ccgtgccagg tgctgtcaga acgcagcggc cagcattcag tggtatcgat
   114721 atgatccggc tgccatacat caaagaacca gccatcagat tccgttctca gacgtttgat
   114781 ctctttacgg aatttgatcg cacgttcaat ggaaccgttg atcagacgct taccagcatt
   114841 gcctttcatc atcgccgctg cggtttcagt ggacgccacg ataccgtagt gcggagaagt
   114901 ggtggtgtgc atcatgtagg cttcgttaaa ggtttcttcg tttacgtcac ctttaacgtg
   114961 gatcatggaa gcctgagaga acgccgccag cagtttgtga gtggactggg tttcgtaaat
   115021 cactttccct tctacacggc caccgctcat accgcattta ccttcgtaaa tcggtgagaa
   115081 gttggtgtaa ggcacccacg cggagtcaaa gtggatggat ttcacatcca gtgttttctt
   115141 gataaagtcg gtgttgtaca gcagaccatc ataggtagag ttggtaatta cagcatgtac
   115201 cggccaggtt gcgtttggtg tttctttcac gcgcttagca atggtagcgt gctggaattc
   115261 actctgtggg ataccaccaa gaataccgta agcgttacgg gtcgggcgga aatagattgg
   115321 cgtaacatcg ctcatcatca tcaggtgggt cagcgatttg tggcagttac ggtcaatcag
   115381 aatggtgctg cctgctggag cagagtacat accaacaatt ttgttcgcag tggaagtacc
   115441 gttggtcacc atgtagctgc ggtctgcgtt aaagacgcga gcgatatact gttctgcttc
   115501 tttgtgtgga ccactgtgat ccagcagaga acccagttca gatactgaaa tggaaatatc
   115561 agatttcatg gtgtttggac caaagaaatc atagaacagg ctacctaccg ggcttttctg
   115621 gaatgcagta ccgcccatgt gaccaggagt acagaaagta tatttacctt cacgaacata
   115681 tttaaacagt gctttagtca gcggaggcag aatagtgttg atatattcgt cagtggtctg
   115741 cttgatctta ttagcaatat cttcagcagc acccagcgca tattcaaaga agctaatctg
   115801 taaacgcagg tcattcaggc ttacatcgag agtggaatac gtattagcga acgcgtacaa
   115861 cggcaggttc tcgttcattt tgctaatttc ttcgcacagc tcgagattat atttatccca
   115921 gtcaaaaata acgccgcaca gacgcgcatt gttttcgatc agttttaata agtcgtcacg
   115981 gtcgttcggg taaacaatct ggaagttcag acgttcaagc gcgcgatgaa gttcacggat
   116041 gggttcttct ttaaaataaa cccccatgtg attcaatatt gcaataacgt tcatagtcat
   116101 atctccaggt aaaaaaggcc cctcccaaca catgggacaa aatgaaagga ggagcctcgg
   116161 aaaatacttt taattaatgt gcgttagacg cggtgtggtt atccattgag tggctctggc
   116221 gctcgtgcat tttgcgagcg tagaacatca ggataatcag gctgacgatg aaggtacctg
   116281 ccagctcgaa ggagcttgcg cccatcagcg cgatgaagca gaacacgcaa cccagtacag
   116341 agcagatcag gctgacaaag ttgcggatgt taacgccttc aaaacgaatc aggtcaacgc
   116401 aagagtagaa gtacggcagc atagtcagca gtactgcgat accggtcagt tcaccgaaca
   116461 ggtcagatgc tttaccaccg gcagagttca tcagagtgat gaggatcatc agggcagtca
   116521 ttttcactgc agccagcagc agaccttttt tcggaatacc gttgctgtcg acttcaccat
   116581 aaactttcgg gaagttaccg tcgttagcgg cacgtacacc tgcctggcct accaacatca
   116641 tccaggagcc cagagaagtc aggcacgcaa aggcggtgaa tgcagaaacc agcggcgcag
   116701 cccagttacc gaggatagtt gaagcactga ttgcaaacgg agcaccggaa gccgccatta
   116761 cagaagacgg atacataccg gaaagcacct gagtcgcagc gatataaaca atacctgcta
   116821 aaccagtacc cagcatggtt gccagcggaa cggtacgttt cgggttttta accataccag
   116881 tacttactgc tgcggattca acacccacga aggcccacag gcagagcaga atacttttaa
   116941 tgatcgcatg accatcagtg gtatccgcag tattccagtt agctgcataa gttgccgcat
   117001 caaaccaatg ccagccaaca atagcagtca tcaccacagg aataagaacc agcaccagac
   117061 caatagtggt taaacggctt acccaggtac cgccgagcat atttacaaag gtaaataccc
   117121 agacgatagc aatacaggcg atacccgccg gaacaggatc atttaatact gggaagaagg
   117181 tggaaagata agatacagcg gtaataccaa tcgccaggtt accaatccag ttagcatggt
   117241 aataaagaac acctgtctga aaaccaaatg caggggaaat ttctccggca taagcaattg
   117301 ggccaccttg ttgcgggttt tttgttgcca gtcgggcata tacatacgcc agcgacattg
   117361 caccaataat agagataatc caaccccaga tagcaatacc accgatactt gctaggttcg
   117421 caggtaataa tgcaataccg ctccccatca tattaccggc aacaacaccg gtacaggcaa
   117481 atagcccgat cttcttggca gaactcatgc tcttctccta atttcatttt tgaatttgga
   117541 gtccgggtca tgatgtataa ctatttcctg accagaccaa actggcgcta agaatactca
   117601 cgaaaaaagg attaatccta aagattaggt gaaataacac aaaagtttct gtaagtgaga
   117661 acttgaggtt ttttattaac acatcaggat cgcaagttga tatcatgaaa agataaacat
   117721 tcaatgttta caatggattg cgggatattc tctggttaaa tttatgtaat aaaaattatg
   117781 cggcaaataa attgccgcaa catattatac caacagaaac atacaaaaac tcaacaacaa
   117841 atattcccga gcataaatca acccgggagt tacttattct gaagcgagaa atttgtcgag
   117901 ataaggtaca acataaggaa cagaagtctg gaatatacca ttttcaatcc agtaaagggt
   117961 gtttgcccct gggcgtaaat taaaggcggt gagatatgca tcagccgctt cccggttcat
   118021 ccccttcatt tcataaacct tgccaagcaa cacataattt agccaggaca tttcaagatc
   118081 aatgccagta tttatcgcct ggtaagactc atctgtttta ccttttacca gagcactgac
   118141 cgcttttatt tgatatataa tggataggtt attcaattcc ggtagtgtaa caatgttgtc
   118201 tatttctgtg ttcagtgctg ctaattgttt ttcatctaaa gggtgttgag aatggcgcac
   118261 gatatcaact aatgcttttt ctgctctcgc gtaggtaaat tctggggatg attgaacaat
   118321 ctcacctaat aattcactgg cacggttcaa tgatttatca tcgccatgca gtaaataatc
   118381 atgtgcctga taaaaattag ttaataacgc accacgatga ggcaaaattt gctggagcgt
   118441 ctcctgcatt cgttgtggcc acggttggtt taatgctttt gataaactct ccagtaaatc
   118501 attttgaatc gccagctgat taccgttagt gatgacataa cgtttatcca gcatggttga
   118561 accatctgca ttgtctacca attttatcga cataaagcat tgttgagcac ggtattggcg
   118621 ctgattaaca aaggcaatag ataatgtttt accggaactg ctcggttcat caatgttgta
   118681 gttgattttg tcatgcacca taaaggtgga gaaggtgtta agtgatgtcg ccaccaaatc
   118741 acccacgcct atcgcgtaag agagctgata cggggaactc cagctgttac aacttttatt
   118801 taccatatta atgtcaatat cgcgtggatt gagcaaaata cgcgatttgc tcataggaag
   118861 acgtgtatca agagttgaaa acgctaccag tgctacacag atacctaacg acaacaggaa
   118921 aaaaaaccat acccaaaagg tagtgaatcg tttgctttta actggggatt gttcaggtgg
   118981 cgttgcggtg ttttgaatgt taagactgtg ggagggagaa tctgtggcag gaaccgcctc
   119041 tggtataggg ggaggcgaag atagcattat ttcctctccc tcttcttcgc tgtaccagat
   119101 aaccggcacc attaatttat agccgcgctt tggtacagta gcgatataga caggactatc
   119161 ttcatcatta tcttttaatg acttacgtag ttctgagata ctctgcgtca caacatgatt
   119221 ggtgacaata cttctcttcc agacattatc gataagttca tccctgctaa gtacttcgcc
   119281 actgtgttga gcaaagaaaa ccagaagatc gattaatctc ggctcaaggg taagttgacg
   119341 cccattgcgg ctaatttggt ttatggacgg agtaacaagc cattcgccaa cgcgaactac
   119401 aggttgttgc ataatagaaa ctcattcgaa aagggaatga tgcaatgata attgccacaa
   119461 cctattttta ccatctatag atgggtttat ttacatatta ttggtgaatg taagacgtta
   119521 tttttaccag ccataaactt cttgattaca tagtattacg aaaggatttt actgagaacc
   119581 agaagtaata ttccttacca tcaaaattca tcatctttgc caaagaaaaa tgttcagaaa
   119641 ataatccatg gaaaattgtc cggagcgctt actattttaa tggattgtta gtctttgcat
   119701 gagcaagcgg actgatacat ttctctttgt tctcattcag aaaatctcat cagtcggcgt
   119761 tcagaccggt gttgtgcttt atggatgcgt tacgctcctg atgacgtcat ttgacgttca
   119821 acaacatcac ggggccgcac gacatttcac gtcagttagt gctatcgctc aggaacaaat
   119881 tttcccgaat tgggatatgc ccgcaaattg ctggtgatgt ggggagaatc tggttgagtt
   119941 cgggagaatt gatttggagg cagaacgctt aaattgtggc gtccagaaac gaaaaacgga
   120001 cctccgtgga ggtccgttga tatgaatttg gtgcccggac tcggaatcga accaaggaca
   120061 cggggatttt caatcccctg ctctaccgac tgagctatcc gggcaacggg gcgcattaaa
   120121 ccgtaatctg cacatctcgt caacctaatt tcagtaaaag cgattcaact gcttaagatt
   120181 gcggcaaatc gcttcttttc tgtgtttctg tcaggtcaat gcaccaccct ggcggcagcg
   120241 ggcgaagcgc aggatatctt ccgccagccg atgtgcagtg tcgacatccg cctggctacg
   120301 attcaccagc attcggctta aacagccttc cagcaccagt tccatctgct ttgctaccat
   120361 cgccggatcg tcaacttcca gcgtggttaa cagttcgtgg gtgaaatcgt aggccgcgct
   120421 tttttgctga tcggccagtt gatgaatagg gtggccagga tcgggataaa acgtacaggc
   120481 agcgataaat agacagcccg gatagcggtt gtttttaacg cactccgata acgcctgata
   120541 acgtgccagc aacttttgtt cggcggtttg cgtttcgtcc agcatcagct gacgacgcca
   120601 gacatctatc tgttggctaa gataacgcag cgcatcgtag aggattgcct ctttgtctgg
   120661 ccagaagcgg cgtagctcgt ccagtggata atccacacgt tcagcaacca tctccagcgt
   120721 ggtgttggca atcccttgta attctaataa tttcagggct tctcccagta catcttcacg
   120781 ttgcacgcta ttttcctccg tctttcccac tgcaagtgtc gttcacggtt ggcgatcgcg
   120841 caaatgtgcg ctgaaggttt cagcatccat aaagcccgtg acgcgtgctt gtggatgctc
   120901 ctggccttgt ccgtcaaaaa agagaattgt cggtaggcca aggacattaa gatgctttaa
   120961 cagcgccaca tcttgtgcgt cgttggccgt gacgttggcc tgaagtaaga ccgtgtctgc
   121021 taacgctttt tgcacctgcg ggtcgctgaa ggtgtatttc tcaaactctt tacaggcgac
   121081 gcaccagtcg gcataaagat ctaacatcac cggtttgcct ttggcttcaa cgagcgcctg
   121141 atttaactca tctaccgttt tgatttgtgt aaagttgaga tgcgtctgag tttgcgcggt
   121201 atgcgtcgca ccaaatgccc aatcctgaag tgggcgcacg ctaaccaatg ccgctgccag
   121261 caggattatt tgcaccacgc gcatccagcc gcgtttagcc tgtaggctgg tgataaaggc
   121321 ccagccaaag aatgcgacac ccagcgccga ccacaagcgt aatccccata catcaccaat
   121381 cactcgctcc agcaggaaga ccggcagtgc gaggatcaca aaaccaaacg cggttttgac
   121441 ttgttccatc cacgggccgc ttttcggcag caagcggtta ccaaagacgg taattagcat
   121501 cagcggcagg cccatgccca acgcatagag ataaagcgtg ccgccgccca gccacatgtt
   121561 cccgctttgg gcgatataca gcagaatcgc gctaagcggt gcggtggtgc atggtgaaca
   121621 gatcagtccg gcaatcgccc ccataacaaa cacaccgcca ggtgagccgc cctgttggcg
   121681 attgctcatc aacgtgagac gtgtttgcag cgaagagggg agttgtagag taaacaagcc
   121741 aaacattgac atcgccagca aggtaaagac gatggcgagg ccaatgagca cgtatgggtg
   121801 ctgtagcgcc gcctggaact gtaaccctgc ggcggcaact accagaccca gcgccgtgta
   121861 agtcagtgcc atcccctgca cataaataaa ggtcagcaac aatgctctgg cagtggagag
   121921 ccgctgttta ccacccagca cgatgccaga aatcagtggg tacattggca gcacgcatgg
   121981 cgtaaaggcg ataccaatac cgatcaacaa cgcccagagc gcggaaaagg gcaattgcgc
   122041 ggtgtgctgc tcttgctgcg aaacagacac aggctgtgac gctgcgttgt tggcgaccac
   122101 ttcgcttaac ggaacggttt tggtttctgg cggataacag aaaccggcat cagcgcagcc
   122161 ctggtaggtg acagttaacg tcgctcccgc actcgcctgg ttgatggtta cgggaagcgt
   122221 cagccgatcg cggtaaatct cgcttttgcc gtaaaactca tcttcatgcc agacgccttg
   122281 cggcagctgc acgtcggcaa ttttcgcgtg ttccggcgta atgcggatct gtttacggta
   122341 gaggtagtaa ccgtctttga tctgccaggt cagattaagg tcatgttggt tttgctgaaa
   122401 atcaaaagta aaggcttgat ccgcggggac aaattgtgaa cgtcccggcg cgtcgaataa
   122461 tccggcaaaa acggaagtgc tgcaaagtag caggatcagc gtaaagatgc gttgagccat
   122521 gagaggtaat ctgtgtctcc gtgtgtaaca ggtaaaacca gaagttccgg ggtttgatat
   122581 ggatgatgag acttcaggca ttccagcagt gcctgctggt gagatacggt agtttttaaa
   122641 atcatctgca cttcgtattc ttgctccagc ttaccttccc agtaatagag agaggtagcg
   122701 ccggggatca aggtcgcgca ggccgccagt ttttccgcca gcactttggc ggctaaatcc
   122761 tgggctgtcg cttcatccgg tgcggtacat agcaccacga cagacgcggt attcgaactt
   122821 ttttcatcaa gcataaacac ctcgcaagaa cagatggaac cgcaagagag aggtcactat
   122881 acaacggacg ggggaaggat gttagtcggc gggaaagaaa gcggggcgtg aacgccccgc
   122941 gattggtcat tacagcatga agctacccag caagaagccg aagcaaacgg ccagggcaac
   123001 acccagagta cccgggatga agaacggatg gttgaagacg aatttaccga tacgggtagt
   123061 acccgtgtca tccatctgta ccgcagcaac cagcgtcggg taggttggca gaatgaacag
   123121 accagacacc gcagcgaaag aagcaacagc ggtcagcgga gaaacgttca gtgccagagc
   123181 catcggcatc agtgcttttg cggttgcagc ctgagagtac agcagagcag aagcaaagaa
   123241 gaagatgacg gccagcagcc acggatgacc ctgaatcact tcaccagcgg tatctttgat
   123301 ccagtcgatg ttgttggaaa cgaaagtatc gcccagccac gcaacaccca ggatacaaat
   123361 acaggcgctc atacctgctt tgaaggtgct ggagttgagg atgttgtcgg tatcgacttt
   123421 acagataacg gtggtcagag ttgcaacgct gagcatgatg atcaggattg cgttggtggt
   123481 gttcatcagc ggtttttcaa ccagacccat gcttgggctg ttgatgattg catagataac
   123541 cacgccaact acgcccagca ggaacagcca gacggacgtt tttgcaccgg atttgatttc
   123601 aatctgcttt tcaccgcgca gttcaaccag gccctcttcc agacgcttac gataaatcgg
   123661 atcgtcagag agtttggagt tgaacagcat agtgaccagg aaggacatca ccagaaccgc
   123721 cagcagggtg gacgggatga ccacggagag cagatggagg tagctgatgc catgaccttc
   123781 catcacggaa gacatgtaaa ccactgccgc tgagattggc gatgcggtga tcgcaatctg
   123841 cgcggatacc actgcagtag acagcggacg gcaaggtttg acgccttgtt ccttcgcaac
   123901 ttcagcgata actggcagtg tcgccagaga tatgttgcca gtaccagcaa agatagtcag
   123961 gaaataggtc acgatcggtg cgaggatcgt gatgtatttc gggttacggc gcagcagctt
   124021 ttctgtctga tgaaccagat agtccagacc gccagcaacc tgcatggcag aaatagcggc
   124081 gataaccgcc atgataatgg aaatgacatc gaacgggatg ttaccgggtt taacgccaat
   124141 agcggcaaga accagcaccc ccaatccgcc tgcaaaacca atacctattc cccccaatct
   124201 ggcgcccaag aagatcgcca gcaaaactat gatgagttct acaactagca tattagcctt
   124261 ccttgttttt taacaagttg atattaggtt gttattttta agttactgct cacaagaaaa
   124321 aaggcacgtc atctgacgtg ccttctttat ttgtactacc ctgtacgatt actgttcgct
   124381 ttcatcagta tagcgttttg ctttgtaagc cgggtgcatc agattctgta cggagaaaat
   124441 atcgtcaagt tccgcttcag tcaacagacc gcgttccaga acgacttcac gtacactctt
   124501 accggtttcg gcacagattt tacccacgat gtcaccgttg tggtgaccga tgaacgggtt
   124561 caggtaagta acgataccga tagagttgta aacgtaacct tcgcacactt ctttgttagc
   124621 agtgatgccg ttaatgcatt tttccagcag gttgtagcaa gcgttggtca gaatgtgaac
   124681 ggattcgaac atggcctggc caatgaccgg ctccataacg ttcaactgta gctgacctgc
   124741 ttctgctgcc atggtaacag tggtgtcgtt accgatgact ttgaagcata cctggttaac
   124801 cacttccgga acaaccgggt ttactttagc tggcatgatg gaagagcccg cctgcagttc
   124861 cggcaggttg atctcgttca ggccggcacg tggaccagaa gagagcaagc gcaggtcgtt
   124921 acagattttg gacatcttca cagccaggcg tttcagcgcg ccgtgaacca taacataagc
   124981 gccgcagtca gaggtcgctt cgatcaggtc ttcagccggt acgcatggga agccagtgac
   125041 ttcagccagt tttttcactg ccagcggaga gtactctttc ggcgtgttca gaccagtacc
   125101 gattgctgtt gcgccaaggt taacttccag cagcagttca gcggtacgtt ggatattttt
   125161 cacttcttct ttcagcagga tgctgaaagc gcggaattcc tgaccgaggg tcatcggtac
   125221 tgcgtcctgc agctgggtac gacccatttt caggatgtcc tggaattcga cagctttacg
   125281 ttcaaagcct tcacgcagtt ggttaatcgc atctaccagc ttaatcaggg aagagtaaac
   125341 tgcgatacgg aaaccggtcg ggtaggcgtc gttagtggac tgacatttgt taacatggtc
   125401 gttcgggttc aggtactgat attcaccttt ctggtgaccc atcagttcca gaccgatatt
   125461 ggccagcact tcgttggtgt tcatgtttac ggaagtacct gcgccgccct ggtagacgtc
   125521 taccgggaac tgatccatgc attttccgtt gttcaggact tcatcacatg cggcaatgat
   125581 ggcattcgct acacttttag gaatggtttg cagctctttg tttgccatag ctgcggcttt
   125641 tttaaccatt accataccgc gaacaaattc aggaatatca ctgattttgt tgttgctgat
   125701 atagaagttt tcaatcgctc tcagagtgtg aacaccatag taggcatcag ctggaacttc
   125761 cctggtaccc aacagatctt cttcgatacg aatgttgttt gacatgtgaa ccttcttttt
   125821 caagctgcca atgatttgct ttaaacacac agaatatatg tggtttcgaa tgtttttcga
   125881 ccgacgatta tcccctgcat cgaccgaata cccgagatca tatgctgctt gaggatttct
   125941 accgtaatct ggatcacttt aagtgtcggt ttttacccct taattattaa tttgtgaaat
   126001 agatcaccgc tttgggatta ctaccaaaaa tagttgcgca aacatcttga aattttgcta
   126061 atgaccacaa tataagctga acgcgattcg caacccattc aggtagccgg gttttaccgg
   126121 ctgctattac aggagaaacc tttgcgctgg ttacctttta ttgccatttt cctttatgtc
   126181 tatattgaga tttcaatctt tattcaggtt gcccatgtat tgggggtatt gctgaccctc
   126241 gtgctggtta tattcacgtc agttatcggg atgtcactgg tacgtaacca gggctttaag
   126301 aatttcgtgc tgatgcagca aaaaatggcg gcgggtgaaa acccagcggc ggagatgatt
   126361 aaaagtgttt cgctgatcat tgccggtttg ctgcttttat taccgggctt tttcaccgac
   126421 ttcctcggtc ttctgctttt attaccgccg gtgcaaaagc atctgacagt gaagttgatg
   126481 ccgcatttgc gcttttctcg tatgccaggc ggcggtttta gcgccgggac cggtggcggt
   126541 aatacttttg atggtgagta ccagcgaaag gatgatgagc gcgaccgcct tgatcataaa
   126601 gacgatcgcc aggattaatg tcgaaacgcc ggattatgtg gttatgccat tttccggcgt
   126661 ttttcgtttt ggcagcaaca gccataaccc cgccagcatg atcagcgcat agagactttt
   126721 ccagccgacc attgccagta acagaacgca taacagcccg ccaaccaccg ccagtagtcg
   126781 ataacgtcct tgcaataatt tacagcctgc cagcatgcat aacagataaa tcataataaa
   126841 gatgccattg gcataaataa taagagcgtc cagattgatc tctaaagcat gaatcaccaa
   126901 cgtgctcacc acacagcagc cgagcaccgc attgagggca ttattcggga tatggcgaga
   126961 agagaggcgt gccaggtagt ggtcaggatt atgttgcgcc tgcgaccaga ccaggcgggc
   127021 gaagctctgt atataaatgt tgagactggc aaagcaggcc agatagccaa tcacgcaggc
   127081 aatccataac gctcctacac cgaacaactg cactacaatt tttggaagcg atgctgccgc
   127141 cgccattttt tcaccatagg cgtcgaagtg taagacgact accgtacagc cccagtagac
   127201 taatcctgcc agcagcagac caatcatcaa tgcgcgagga aaatcacgct ctggattttt
   127261 aaattccgag gcaagatggg caaatgcttc cagaccgaca aaacaccaga acatcactga
   127321 taacgcagca aataacccgg taagttcgat attaccaggt gccgggaagg ggatattcgc
   127381 aggtttgata tcgcccgccc accagatagc gacaatcagc gcgacgataa gtccggcaat
   127441 aacggtttgt agattagcac tggaactggc accgcgagta ccgatatacc acaccagcgc
   127501 cagcgtaccg agttctgcca acaacagttg ccagctatgc cagccaaaca ttgcctggcc
   127561 gaacccggcg gcaatttgta gcgcggcagg caaacccacg ggaatgaccg ataaaaacaa
   127621 ccagccggtg actcgctcaa gtcgcgaacc aaacgccata ccgacgaagt gcgcgacacc
   127681 gcctgcgctg ggatagtggc gacccagaat cgcaaacaca atcgcaatcg ggaacactaa
   127741 gataatcaaa acgggccacg cccacaggct gttattgccc gctaccagcg cagctaacgc
   127801 aggaacggca aacacgccag tgcctaataa tgacgtcgat agcagtccaa tgccctgggc
   127861 cagccccagt tcttgtttga gtccactcat gggttgatat ccgattgcgc ccaaattttg
   127921 ggcaactgcg tagattttcg atggtagcac aatcagattc gcttatgacg gcgatgaaga
   127981 aattgcgatg aaatgtgagg tgaatcaggg ttttcacccg attttgtgct gatcagaatt
   128041 ttttttcttt ttcccccttg aaggggcgaa tcctcatccc catttctctg gtcaccagcc
   128101 gggaaaccac gtaagctccg gcgtcaccca taacagatac ggactttctc aaaggagagt
   128161 tatcaatgaa tattcgtcca ttgcatgatc gcgtgatcgt caagcgtaaa gaagttgaaa
   128221 ctaaatctgc tggcggcatc gttctgaccg gctctgcagc ggctaaatcc acccgcggcg
   128281 aagtgctggc tgtcggcaat ggccgtatcc ttgaaaatgg cgaagtgaag ccgctggatg
   128341 tgaaagttgg cgacatcgtt attttcaacg atggctacgg tgtgaaatct gagaagatcg
   128401 acaatgaaga agtgttgatc atgtccgaaa gcgacattct ggcaattgtt gaagcgtaat
   128461 cctcgcacga cactgaacat acgaatttaa ggaataaaga taatggcagc taaagacgta
   128521 aaattcggta acgacgctcg tgtgaaaatg ctgcgcggcg taaacgtact agcagatgca
   128581 gtgaaagtta ccctcggtcc gaaaggccgt aacgtagttc tggataaatc tttcggtgca
   128641 ccgaccatca ccaaagatgg tgtttccgtt gctcgtgaaa tcgaactgga agacaagttc
   128701 gaaaatatgg gtgcgcagat ggtgaaagaa gttgcctcta aagcgaacga cgctgcaggc
   128761 gacggtacca ccactgcaac tgtactggct caggctatca tcactgaggg tctgaaagct
   128821 gttgctgcgg gcatgaaccc gatggacctg aaacgtggta tcgacaaagc cgttaccgct
   128881 gcagttgaag aactgaaagc gctgtccgta ccgtgctctg actctaaagc gattgctcag
   128941 gttggtacca tctccgctaa ctccgacgaa accgtaggta aactgatcgc tgaagcgatg
   129001 gacaaagtcg gtaaagaagg cgttatcacc gttgaagacg gtaccggtct gcaggacgaa
   129061 ctggacgtgg ttgaaggtat gcagttcgac cgtggctacc tgtctcctta cttcatcaac
   129121 aagccggaaa ctggcgcagt agaactggaa agcccgttca tcctgctggc tgacaagaaa
   129181 atctctaaca tccgcgaaat gctgccggtt ctggaagctg ttgccaaagc aggcaaaccg
   129241 ctgctgatca tcgctgaaga tgttgaaggc gaagcgctgg caactctggt tgttaacacc
   129301 atgcgtggca tcgtgaaagt tgctgcggtt aaagctccgg gcttcggcga tcgtcgtaaa
   129361 gctatgctgc aggatatcgc aaccctgact ggcggtaccg taatctctga agagatcggt
   129421 atggagctgg aaaaagcaac cctggaagac ctgggtcagg ctaaacgtgt tgtgatcaac
   129481 aaagacacca ccactatcat cgatggcgtg ggtgaagaag ctgcaatcca gggccgtgtt
   129541 gctcagatcc gtcagcagat tgaagaagca acttctgact acgaccgtga aaaactgcag
   129601 gaacgcgtag cgaaactggc aggcggcgtt gcagttatca aagtaggtgc tgctaccgaa
   129661 gttgaaatga aagagaaaaa agcacgcgtt gaagacgccc tgcacgcgac ccgtgctgcg
   129721 gtagaagaag gcgtggttgc tggtggtggt gttgcgctga tccgcgtagc gtctaaactg
   129781 gctgacctgc gtggtcagaa cgaagaccag aacgtgggta tcaaagttgc actgcgtgca
   129841 atggaagctc cgctgcgtca gatcgtcctg aactgcggcg aagaaccgtc tgttgttgct
   129901 aacaccgtta aaggcggcga cggcaactac ggttacaacg cagcaaccga agaatacggc
   129961 aacatgatcg acatgggtat cctggaccca accaaagtaa cccgttctgc tctgcagtac
   130021 gcggcttctg tggctggcct gatgatcacc accgagtgca tggttaccga cctgccgaaa
   130081 aatgatgcag ctgacttagg cgctgctggc ggtatgggcg gcatgggtgg catgggcggc
   130141 atgatgtaat tgccctgcac ctcgcagaaa taaacaaacc cccgggcaga aatgtctggg
   130201 ggtttttctt ttggtcatct ttctagtata agattcagac acggacgacg cgagtggcgt
   130261 ccagctcatt gattatgggg aataacatgc acgtaaaata cttagcaggg attgtcggtg
   130321 ccgcgctact gatggcgggt tgtagctcca gcaacgaatt gagtgctgcc ggtcagagtg
   130381 tacgcattgt ggacgagcag ccaggcgcag agtgccagct gattggtact gcgacaggta
   130441 agcaaagcaa ctggctttcc gggcaacacg gagaagaagg cggttctatg cgcggcgcag
   130501 caaacgatct gcgcaaccag gcggctgcaa tgggcggtaa cgtgatttat ggcatcagta
   130561 gcccgtcgca gggaatgttg tccagttttg tcccgacgga tagccagatt atcggccagg
   130621 tttataagtg cccgaactga tgtgatggtg acaaacgtat aattgccaga tgcgcttcgc
   130681 ttatcaggcc tacataatgc attgcaactg attgaatgct tgagaatttg taggccggat
   130741 aaggcgttta cgccgcatcc ggcatgacca acgatcacgt tatcagtcat ttcaggctgg
   130801 atggctccag cctcgcatta gcaaagatga gattatttcg cctgtggtgc agttttgctg
   130861 gtggattgaa cacgtaagtg atgcagcgcg cgtaagcatt gatcctgggt tagcggttgg
   130921 gcgttattcg ccgctacggt gcgaacatag acctgacatg gcacacccac gagttttttc
   130981 agtcgtgggc tgaaatcaag cattcggcga gtgatcgcct ctacttcttt acccggcgtt
   131041 gcttcacgcg ttttcaccac tgcgccgcta aattgttctt tgccactttc atctttaaag
   131101 cggtaaagca ccgccagata atggtcaaac aagttatgtt tccattccgg ttgggaatag
   131161 gtgtcatatt ccagattgcc attttcctgg cagtcaacgt catataacgg caggaaatct
   131221 aacagtgagg taatacgtaa tgccagttca cgcatttccg cattattaat tgcatgaatg
   131281 attgctcgcg ccagcatttc aatctgcaac tcattgacga ctaactcaca tttgttaccg
   131341 tcatgtagcg ttaatacgaa ggtaagattt tcgccttgat tatcggtaag ttccagcgtg
   131401 ttaacccgac gattaatatc ggcattttta agctcatcaa ccagaatttc tgggatgttt
   131461 tctgccattt tcttaatgac tttatcgcga gcttgctcat actgaaggcg tgcagcagca
   131521 tccagtttat gtttttgatg cagacgactt tccagcgcaa tcagcaggtc gcgcagttcc
   131581 atgacagaca taaagaacaa cgattcttta ttgcgaggtt ctttaatttt cagcgccaat
   131641 gcaatgaaat tattactctt gcgaataaca ccggtattaa cacccttgat gcttatggcc
   131701 atgtaggttc tccctaacca tttctcaatt aaataattaa ttttaattta taagccagat
   131761 aaatgggctt ggtagtaata gttgttaaaa taacataaat agccgtactc actctatata
   131821 aacagaaaag aaaggttaat tattggtgtt agttatataa agagtaacaa cagcaatgta
   131881 tatgaataat taaaggttat gcaacgggca aagattaaat ttcccagttt aatgacaggt
   131941 tgctgtactt gcgtttgttg ctcttgccgg atgcggcgta aacgccttat ccggcctaca
   132001 gttcattgca aatccattca atttcaatgc acgatgtagg cctgataagc gtagcgcatc
   132061 aggcaatttt aatgtttgac ttccctgttt aatcagttac tgctggcgta gctggagatc
   132121 cagcggcgtt ttgctgggtt cgccgccaat ttctcgcgcc agtttcggca ccagatatcc
   132181 cgacaccagt gtcagcaact cacgcataat ctgccgtgct tcgtcatcac tcaccataaa
   132241 atgcgccgcg ccctgtactt tatcgagcac atgcaggtaa tacggcatta cgccggcatc
   132301 gaacaacgca ttactcaggt ttgccagcgt ttgtgcgtta tcgttcacac cacgtaacag
   132361 aacgctctgg ttaagcaggg tgacaccgac acggcgcaat ttagccatcg cctgacggaa
   132421 cgtttcatct acctcattgg catgattgat gtgattcacc agcaagattt gcagcgtaga
   132481 acgggcaaag cgttcaacca gcgcctcggt gatacgtgcc gggatcacaa tcggcagacg
   132541 gctgtgaatc cgcagacgtt tgatatgcgg gatggcttcc agttgtgtga gcaaccagtc
   132601 cagctcgtga tctttcgcca tcagcggatc gccgccggag aaaatcatct cgtccagttc
   132661 cggatgcgca gcaacatact caagagcagt ctgccagtta cgcttgttgc cctgattttc
   132721 ggcatagggg aagtggcgac ggaagcaata gcggcaattt accgcgcagc cgcctttgac
   132781 cagcaaaagc gcccggttgt ggtatttatg caacaaacca ggcactacgc tgtgctgttc
   132841 ttccagcggg tcggtggaga atccggacgc gacgacaaac tcatcttgcg aggtaagtac
   132901 ctgacgcaaa agaggatcgt ccggattgcc tttctccatg cgatcgataa atgagcgggg
   132961 cacacgcagg gcaaacagct ttttggcgct gcgtccggcc aacagttttt cgtccgcgtc
   133021 tatattcaaa agacgcagaa gttcatcagg atcggtcaca acatcggcaa gttgcgttaa
   133081 ccaatcttct ctggatgggg tatttagggt tacaatatgc gccattttgt ggcttagcta
   133141 ccaattaaca aatttcagag ggccttatgg caacgtacta tagcaacgat tttcgtgctg
   133201 gtcttaaaat catgttagac ggcgaacctt acgcggttga agcgagtgaa ttcgtaaaac
   133261 cgggtaaagg ccaggcattt gctcgcgtta aactgcgtcg tctgctgacc ggtactcgcg
   133321 tagaaaaaac cttcaaatct actgattccg ctgaaggcgc tgatgttgtc gatatgaacc
   133381 tgacttacct gtacaacgac ggtgagttct ggcacttcat gaacaacgaa actttcgagc
   133441 agctgtctgc tgatgcaaaa gcaattggcg acaacgctaa atggctgctg gatcaggcag
   133501 agtgtatcgt aactctgtgg aatggtcagc cgatctccgt tactccgccg aacttcgttg
   133561 aactggaaat cgttgatacc gatccgggcc tgaaaggtga taccgcaggt actggcggca
   133621 aaccggctac cctgtctact ggcgctgtgg ttaaagttcc gctgttcgta caaatcggcg
   133681 aagtcatcaa agtggatacc cgctctggtg aatacgtctc tcgcgtgaag taatgcggtt
   133741 gtggtgcggc ctgcaggctg caccatcact tattcaggtc agagatgatg aaacgcctta
   133801 tcgttcttgt tttgcttgcc agcacgctgc tcacgggctg taacaccgct cgcggtttcg
   133861 gcgaagacat caaacatctc ggcaactcca tctcccgcgc tgccagctaa tttttcttct
   133921 cttccgaaaa atcatcagat ttccatcatt tttggcgatg ttgtctatta ttaatttgct
   133981 ataggcaaac ataaataaca ttacctaaaa ggaagacgtt atggtgaaga agacaattgc
   134041 agcgatcttt tctgttctgg tgctttcaac agtattaact gcctgcaaca ccacgcgtgg
   134101 cgttggtgaa gacatttctg atggcggtaa cgcgatttct ggtgcagcaa cgaaagcgca
   134161 gcaataagca ataacggtac gacagttatg tcgtgccgtt tgtttttttg cgatagtcac
   134221 aaaggtaata gttgaaattc ccctgccacc tggcaaaata tccgttcaac catcagcttt
   134281 gcaggacgac ctgcaaacgc ctcttttcac cggggacggc cccaattctc cggagcctga
   134341 tatgtcctgg attatcttag ttattgctgg tctgctggaa gtggtatggg ccgttggcct
   134401 gaaatatacc cacggcttta gccgtttgac gccgagtgtt attaccgtga cggcgatgat
   134461 agtcagtatg gcgctacttg cctgggcgat gaaatcgtta ccggtaggga cggcttatgc
   134521 cgtgtggacg ggtattggcg cagtcggcgc ggccatcacc ggcattgtgc tgctcggtga
   134581 gtccgctaac ccgatgcgcc tggcgagtct ggcgttaatc gtattgggga ttattggtct
   134641 gaaactcagc actcactaac taccgggctg ctgtacccaa ataaacttac tgacatcaaa
   134701 cccttcccgg gtcgcgactg ccagcatctc ctgtttcact tcgtcagaaa tggttggcgt
   134761 gcgggagagt atccacaggt agtcgcggtc cgggccgcaa accagcgcat ggcggtattc
   134821 ccgatcaagt gcaatgacgt tataaccgcc atagaaagga ccaaagaatg acactttcag
   134881 cgcagcgcgt gttggtgcgc cggtaaagta cgctttccct tcactctgct gccacatttc
   134941 tctgtcaggg ttatagcctt tattgatgac attcaggccg ccgtcatcac gcagactgta
   135001 tgttgcggtg actttttcca gtccacgttc aaaacggtga tcaaaacggg caatctcata
   135061 ccaggtaccg agataacgtt ttgcgtcgaa attattcact actgttacgc cacgcggcgg
   135121 cgtaggagaa ctgcaggcaa cgaccagaaa tgcagctgtc gctgcggcaa cgagagggag
   135181 caggcgcata aatgtttcct tactggtttt tttctaagtg tagatgacag caaggaaaag
   135241 cgcagaaaag gtccgaaaat tcggacccga tgaaatttta ctgtagagcg ttgagaatct
   135301 gccaggcggc ggcgactctc gctggattgg gatagttttt gttagccagc atcactatac
   135361 ccagctcttt ttctggaata aacgcgacat agctaccaaa tccgccggtt gcccctgttt
   135421 tatgtaccca tgatgcgcgt actgcaggag ttgggggcgt aatcgctttt acagggtgtg
   135481 ctgccagtgc aattttattg ccactgccgt taatgatgct gtcaggattt accggccagt
   135541 ccagcatttc ccagcccagg ccctgataca tatcgccggt ttgccagtag cgagattgtg
   135601 ccagttgtat cccttgctga agtgttttgt cgttgatatc acggggattc atattgcttc
   135661 gtacccagcg ggccatatct tcaatggtcg acttcacacc ataagcttca gcatctaacg
   135721 cccctggcga aacatgcact gccttacctt cgcgatatcc ccaggcgtaa ttcttttctt
   135781 ctgggggagg tacattaatc cacgtatggt tgagtttgag tggctggaag acacgagttt
   135841 gcatcgcctg ctcaaaactc aaaccagacg gcttcacagc cagtgcgccg aacaaaccga
   135901 tactggagtt ggcatacaga cgttgtgttc ctggagccca tgcaggctgc cagttttgat
   135961 agaagcgcag caagtcgctt gaggatttca cttcatccgg cacctgcaat ggcaggccgc
   136021 cagcggtgta ggtcgcaaga tgtaatagtg tgatcccatt ccactgttta gcggtaagtt
   136081 caggccagta ttttgttgcg ggatcgctta acttgatttc ccctcgagca atagcgtcgc
   136141 caccaagcac gcccgtaaat gttttgctga ccgaacctaa ctcaaacaac gtttgctgtg
   136201 tgacgggctg ctttttggcg atgtccgcat agccccaggt aaagtaataa ggtttaccct
   136261 gataaattac cgccaccgcc ataccgggga tcttttgttg ctctataagc ggggtaattg
   136321 tgcgatgcac aatatcgttg atttgttgag gggcagcaaa tgtggagcaa gaggcggtaa
   136381 ttaataaggc gcagagcgtc gttttgaaca taaggtctgg tttccataca aaacggcccg
   136441 ccataggcgg gccggattta cattggcgat acgttagatt gtaacgatac caatcagcgt
   136501 gacaactgtc aggatagcag ccagaccgta gaaaacccat ttgcccgcag gcacgtgaat
   136561 tttcagatcg tgcatcgcgt ggtgcatacg gtgtaaacca caccacagcg gcagaacgat
   136621 catcaggaac aggaatacgc gaccaatgaa gctctgcgcg aacgccagaa cgcgctcgta
   136681 gctcagcgca tcacccggaa acagccccag tggcagcaga atacccacca gcaggatcat
   136741 caccggcgca ataattgcgc tccacatacc accggccccg aagaggcccc agaataccgg
   136801 ttcgtcagaa cgctttggat ttggattaat catctcaggc tccttaccag tacagggcaa
   136861 caaacaggat tacgatggtg gcaaccacag ttaccgccca gagacttttg ataattggct
   136921 ctggtcccat tttttcgtct tttacaatga tattggccgc tttcggtgcc agttcaaacc
   136981 aggttttggt gtgcagcaga gctgccgcca gagtgatcag gttaatgatc acgataaccg
   137041 ggttttgtaa aaagtcgacg aatcccgccc aggcttccgg gccatttttc agggcaaaca
   137101 gcccgaaaat cagttcaatg ctgaaccaca cagccggaac cgccgtgcct tcgcgcagca
   137161 tgtaaaagcg ataaaacggc aattttttcc accaggtgga cgtcattggc cgtacatacg
   137221 gtttacgttt agtcgtcatg ttgcactcct tagcgtggtt tcagggtcgc gataagaaag
   137281 tctttcgaac tttctacttt gccctgctga atggccgcag ccggatcgac gtgtttcggg
   137341 cagacttcgg agcaatagcc cacgaaagta cagctccata cgccgttctg gctgttcaac
   137401 tgcgccatac gctccttctt accgtggtcg cggctatctt cgttataacg atgcgccagc
   137461 gtaatggcag ccggaccgat gaactctggg ttcaggccaa actgcgggca cgcggcgtag
   137521 cacagaccac agttgatgca accggagaac tggtgatact ttgccatctg cgccggggtc
   137581 tggatgttag taccctgatc cgcggtgcgg gagttgccga tgatgtacgg tttgatcgct
   137641 tccagacttt cgatgaagtg ggtcatatcg accaccagat cgcgttcaat cgggaagtta
   137701 gctaacgctt caaccttcat accgtcggtg taatcacgca ggaaggtttt acatgccagt
   137761 tttggcacgt tgttaaccat catgccgcag gaaccacaaa tcgccatacg gcaggaccag
   137821 cggtaactca ggtccggtgc caggttgtct ttgatgtagc ccagcgcatc cagtaatgag
   137881 gtagttgcgt cataaggcac ttcatagaat gcgctatgcg gtgcggtatc gacttccggg
   137941 ttatagcgca ccacctcaat tttcaggttt ttcatctcag ccattcgcct tctccttctt
   138001 attggctgct tccgccttat cggctgcatc cgcttcgcca ccgtaaacgc gtttagctgg
   138061 cggcagcgta gtaatcttca cgtcgctgta ctccaggcga gtcgtgccat cagcatcgcg
   138121 gaaggcgagg gtgtgtttga ggaagttgac gtcgtcacgc tcggtgcaac cttcgtccag
   138181 acgctggtgt gcgccgcggg actctttacg tgccattgcg gagtgcgcca tacattcagc
   138241 aacgttcaga ccgtggccca gttcaatggt gtagagcagg tcggtgttga acacgctgga
   138301 ggtgtcggtg atgcgcacgc gcttgaagcg ttcctgcagc tctgccagct tgtcgatggt
   138361 tttctgcatc agttccggcg tacggtagat accgcaacct tcttccattg ccaggcccat
   138421 ttcgtcgcgg atcttcgccc agttttcgcc gccatcctgg ttaaccagat ctttcagacg
   138481 ttgttcaacg ccagctgcct gcgcttcaat tgccgcttcg ttgccattac cggcagttgc
   138541 tgcacgctct gtcgcttgtt caccggccag acggccgaag accaccagtt ccgccaggga
   138601 gttggagccc agacggtttg caccgtgcag accaacagag gaacattcac ccacggcgaa
   138661 cagaccttta atgcgggttt cacagttctg atcggtttcg ataccgccca tggtgtagtg
   138721 tgcggtcgga cgtaccggaa tcggttcttt aaccggatcg acgccaacgt acgctttcgc
   138781 cagttcgcag atgaacggca gacgttcatg cagttttttc tcgccgaggt gacgcaggtc
   138841 gagataaacc acatcgccac gcggcgtgga gatggtgttg cctttacgcc attcgtgcca
   138901 gaaggcctga gaaactttgt cgcgtggacc cagttccata tatttgtttt tcggctcgcc
   138961 cagcggagtt tccgggccca tgccgtaatc ttgcagataa cggtagccat ttttgttgac
   139021 cagaataccg ccttcaccgc ggcagccttc ggtcatcagg ataccggaac ctggcagacc
   139081 ggttgggtga tactgaacga attccatgtc acgcagcgga acgccgtggc ttagcgccat
   139141 acccataccg tcaccggtaa cgatgccgcc gttggtgttg taacgataaa cgcgacccgc
   139201 accgccagta gccataacga ccgcgttagc acggatctgc accagcgtgc cttccatcat
   139261 gttcattgct accaggccgc gaacatgacc atcatcaacc agaatatcca gcacgaaatg
   139321 ttcgtcaaaa cgctggatct gcgggaattg cagagaggtc tggaacagcg tgtgcagcat
   139381 atggaagccg gtcttatcgg cggcgaacca ggtgcgctcg attttcatgc cgccgaagcg
   139441 acgtacgttg acgctaccat ccgggcgacg gctccatggg catccccaca gttccagttg
   139501 ggtcatttcg gttgggcagt ggtggacgaa ataatccacg acatcctgct cacacaacca
   139561 gtcgccaccc gctactgtat cgtgaaagtg atattcgaag ctgtcatgat cctgcgcgac
   139621 agcggcggag cccccttctg cagcaacggt atggctacgc atcgggtata cttttgagat
   139681 tagtgcgatt tttgcattcg gatttgcctg cgcggcagca attgcagcac gtaatcccgc
   139741 gccaccggcg cctacaatgg caagatcggc ttgaaaggtt tgcacgacat tcctccagat
   139801 tgtttttatc ccacagccac gtacttcagg gtaagtacct gaaagttacg gtctgcgaac
   139861 gctattccac tgctccttta taggtacaac agtatagtct gatggataag tctgaaattt
   139921 gacgagatcg atttttttag tgcgcaagag ggctaaatta tcactgaaga tgattaattt
   139981 aattactaaa ccatcagatc gtgctttttt tagtcactgc ccaccgctgt ttgattcctg
   140041 cgtaaaattt gtctcgccgt cgcgttgcga gtagacttcg tgcccttgtc aaaaactgga
   140101 gatttaacta tgagcgaaac ggcatcctgg cagccgagcg catccattcc taacttatta
   140161 aaacgcgcgg cgattatggc ggagatccgt cgtttctttg ccgatcgtgg agtgctggag
   140221 gtggagacgc cttgtatgag ccaggcgacg gtaaccgata ttcatttggt cccgtttgag
   140281 acacgtttcg ttggccccgg gcattcgcag gggatgaatc tctggttaat gaccagcccg
   140341 gaataccata tgaaacgcct gctggttgcc ggttgtgggc cggtattcca gctgtgccgc
   140401 agcttccgta atgaagagat ggggcgttat cacaaccctg agttcactat gctggagtgg
   140461 tatcgaccgc actatgatat gtaccggttg atgaacgagg tggacgatct cttacaacag
   140521 gtgctggact gcccggcagc agaaagcctt tcttatcaac aagctttctt gcgttatctg
   140581 gaaattgacc cactctctgc cgacaaaacg caactgcggg aagtggcagc gaaactggat
   140641 ttgagcaatg ttgctgatac cgaagaagac cgcgacacgt tgctacaatt gctgtttacc
   140701 tttggcgtag agccaaatat tggtaaagaa aaaccgacct ttgtgtacca ctttccagcc
   140761 agccaggcat cactggcgca aatcagtacc gaagatcatc gggtcgctga acgctttgag
   140821 gtttattata aaggtattga gctggcgaat ggtttccatg aattgacgga tgcccgtgag
   140881 cagcaacaac gctttgaaca agataaccgt aagcgcgcgg cgcgcggttt gccgcagcac
   140941 cccattgacc agaatctgat tgacgccttg aaagtcggta tgcctgactg ttccggcgtg
   141001 gcattaggtg ttgatcgtct ggtgatgttg gcgctgggcg cggagacact ggctgaagtc
   141061 atcgccttta gcgttgaccg ggcataattc tgaaaaatta ctgaattaac agcgaagaat
   141121 ggcgtaataa gcgccattct gtagcatatg ttttcatttt tccgctaaag gccacaattt
   141181 aagactggat atttgatatc atccaggtat caattctgta ttggttttta cgctgcctgc
   141241 tcaatgttgc gcagtgcagt tcagtgggca cgtgttatac acgcgctgaa atgaaggatg
   141301 gtttcatgcc tcacacgata aaaaaaatga gtctgatagg actcatattg atgatcttta
   141361 cttccgtatt tggttttgcc aatagcccat cggcttatta cttaatgggt tatagtgcga
   141421 ttccctttta tatattttct gcattgttat tctttattcc attcgcctta atgatggctg
   141481 aaatgggagc tgcttatcgc aaagaagaag gtggtatcta ttcctggatg aataatagtg
   141541 tcggaccacg ttttgccttc attggtacgt ttatgtggtt ttcctcttat atcatctgga
   141601 tggtgagtac ctccgcgaaa gtttgggtac cgttctcaac attcctctat ggtagcgaca
   141661 tgacccagca ctggcgtatt gccggactgg agcctacgca ggtggttggt ctgctggcag
   141721 tggcatggat gattctggtc accgtcgttg cttctaaggg gattaataaa attgcccgca
   141781 ttactgcggt gggcggtatt gcagtaatgt gtctgaattt agtattgctg ttagtaagca
   141841 ttactatttt gttattaaat ggtgggcatt tcgcgcagga tattaatttc cttgcatcac
   141901 cgaacccggg ttatcagtcc ggtctggcaa tgctatcgtt tgtggtattt gccatttttg
   141961 cctatggcgg aattgaagcg gttggtggtc tggtcgataa aacggaaaat ccagaaaaga
   142021 actttgccaa aggtattgtt tttgccgcta ttgttatttc aatcggttat tcgctggcaa
   142081 tatttttatg gggcgtcagc acaaactggc agcaggtatt aagtaatggt tccgttaacc
   142141 tcggcaatat tacctatgtg ctgatgaaga gccttgggat gacgctgggt aatgcactgc
   142201 atttgtcacc tgaagcgtca ttgtcgctgg gcgtatggtt tgcgcgtatt actggacttt
   142261 cgatgttcct cgcctatacc ggtgcgttct ttacgctttg ctattcaccg ttgaaagcca
   142321 tcatccaggg gacgccgaaa gcattgtggc cggaaccgat gacgcgcctg aatgcgatgg
   142381 ggatgccgtc tatcgccatg tggatgcagt gcgggttggt tactatcttc attctgctgg
   142441 tttcgtttgg tggcggtacc gcatcggcgt tctttaacaa gctgacgctg atggcgaacg
   142501 tgtctatgac gcttccttac ctgttcctcg cgctggcttt cccattcttt aaagcacgtc
   142561 aggatctcga cagaccattt gtgattttca aaacgcgtat gtcggcaatg atcgcgacgg
   142621 tggttgtcgt actggtggtg acatttgcga acgtcttcac catcattcaa cctgtggttg
   142681 aagctggaga ctgggacagc acattgtgga tgattggcgg ccctgtcttc ttctcgctgt
   142741 tagcgatggc gatttaccag aactattgca gtcgcatggc gaataaacct gagttagctc
   142801 tcgactgatc atatgaaatc ctgcaactca tcgtctctcc ggcgatgagt tgtcaggaag
   142861 taatgcgtat ggatgatact ctccagggcc ggtgataact gaagatgatg ttaagctgct
   142921 aaatgagcca cgttgacatc atttggcagc ttcatgcatt acaaacttcc cgcctgacga
   142981 cctttgcccg cagaagttaa tgttctcccc gtttgtttac cattcaggct ttccagacgc
   143041 atctggaagg gcgggaatgg catatcgata ccgtgggcat ggaagcctgc cagaatcagc
   143101 tggtggatct catggcgtag cggcatacgg tgacccatct cagcggcgta aatacgcagc
   143161 tcgaaaatct gaatcccttg ttgcagatcc accaggaaga cttccggtgc cgggttgtcg
   143221 atcaccagcg agcagcgacg cgctgcggtg agcaggattt ccgtcacttc ttcactgttg
   143281 gcatcggcag gggccggaat cgtcaacacc acgcgcgtga ccgagtcaga gagcgaccag
   143341 ttgataaact gctcggtaat aaacgccttg ttcggcacga ttatctcttt acggtcccag
   143401 tcgctgatgg tggttgcgcg ggtattaatt ttcgtcacgc taccagtaag atcgcggatc
   143461 gttaccgtat cgccaatgcg aatcggtttt tcgaacagga tgatcaggcc agagataaag
   143521 ttggcgaaaa tttcctgtaa accaaaaccg agaccaacac cgagcgcggc aaccagccac
   143581 tgcaatttcg accactcaat accaatcatt gagaagccga ccagcccgcc aatcagcatc
   143641 agcagatatt tggtgatggt ggtgatggcg taacccgtac ccggcgttaa atccaggtgc
   143701 tgcaaaatcg ccagttccag cagcgcgggc aggttgcgca ccagctgcgt ggtgatgata
   143761 aacaccagaa tggcaatcag caccgcaccg agggtaattg gctccagact ttctacgccc
   143821 tgtaccgtgg aggtgacatc ccacagcgaa atattttcga ggaagccgaa agcggaatgg
   143881 atttctgacc acagcacaat gaccgaaagc agggcgatca acatcaaaat tgagcgcacc
   143941 agccgcaagg attgcgcact gatggcatcg agatcgactt cgctttcatc gacttcaatt
   144001 gccccttccg ggctactgtg atgatgtgct tcctcttcgc cacgcgcacg ttgcgctaac
   144061 atctctgccc ggcgatgctt cgcccggtca aacgccagcc tgcggcgttg gatcagcatc
   144121 cagcggcgga taacgtgata aaccaccagt agcaggaacc agatggcaac cgaggtttca
   144181 agcctcgcta acagcgcctg tgccgttgcc agataaccca ccgccgacgc cagaatggca
   144241 accagtggcg cgccaatcat catgttccac agcatatggt tggtaatgtt gtcgccgctg
   144301 ccctctttgt tgagatacag tgggatcccg gcctttttca ggctgagggt gaccaccgcc
   144361 agcgcaccgc aaatgaggat aaagcaaagc cgtcccagcg aaccggagaa ttcacggtcg
   144421 tcgaggttat cgaacatcat cagcgccata atcagcggca caataagccc gatgctcatc
   144481 aggtagtagc gcatcccacg ggaaacacgt tcgcgcggcc agccaaaatg agcgataaac
   144541 aagccgttcg ggcgggcaaa ggtggcgcaa atcatcacta cccacagcag cggcacggtg
   144601 gccgttacac catcgccaat cgcgaccgcc agcggataag gccacgcctc gcgcaagccg
   144661 taacccagcg tcatccacag caccggtaac ggtgacgcga cgagaatcga ccagaaaaga
   144721 gtgcgcaacg tcagccagaa gtgatcctga gtcactttgc caactttcgc cgccgaacgt
   144781 tcaagaaaac gggtgaaata gcggcgcgag taaatactgc aaccgaccag aatcaacgcg
   144841 ccaaacagcg gcaaaattgt ctctttgctg gtcagcatca tcacactggc tttgcccaac
   144901 tgactgaagg tgtccagcga aatgagacga cgcagatcct gggcgatttc cagcggccag
   144961 gcgatggtca tcgggcgcac gtcagaggtc cagaacagat agcggtgcgt tgcttcgttc
   145021 acctctttca gcgcatcctc cagttgcccg ttggagactt tcagcttggt cagttccaat
   145081 agtagcgtgt cgccaccctg caacaatgag ttcagcaact cacgctgagt gcgcagttgt
   145141 gcttccagaa tacggttttg ctcggcggtc agcggctgac cgtcggcctg atggatttgc
   145201 cgtagcagcg gctgtttatt aagcagatcc tcataacgta accgttgcac acgcaactgc
   145261 gccatttcgg tatcaagctg ttgtggtttt ggcatttctg gcagccgtgc cacctgcgcc
   145321 cgcagcgctt cgccgagcag attggacgat cccagccatt gcgactgttc acgcagcgta
   145381 ttcaacgcct gccggacctg taacgtctgg ctggcagcct gacgctgttg cgaggcaacg
   145441 agatccatcc gctgcgcctg ttgattcaaa gccgccgata gttcgcggtt aattttgaat
   145501 tgcgcgacga tatctttcgg caaatcggca ctgttttctg ccagtaattc ggtactttcc
   145561 agcgcccgct ccgcctcaag ctgacgttgg ctgtttaatt gattacgcaa ggcctgcaaa
   145621 tacgcatcca gttgctggct ctctttttcc gccagctctg agcgtaagcg cgctaattcc
   145681 tggcggttat tggcagacag ctgcgccagc tccagttcat caacgagcgc cttaagacgt
   145741 gcagagtcag actgcaacgc gaaattttgt gcctgattga gcggagtatt gccagtaagc
   145801 gtccccaggc ggcgctcgat ctcatttaac tgacggcggg cgtcggtttg ctgttgcggc
   145861 agttgattca gcgaatcggc aatctcgcgg gcgcgctcct gctcttgctg ggcctgacga
   145921 cttttatcca gcaactggct gctgacctgg agaatttcct gattcagcgc gtcggtagac
   145981 attcccggcg acacgctgcg cggctcgtca cgcatgttgt ttaattgtgc gcgcagagta
   146041 gcggagagtt tcggataatt atcgataact tgctgatatt gtttgatgcg ctcaagggaa
   146101 ccttttcgtt cctcaagcgc atttaaggca gactggagcg cctctacgac ttccggctgt
   146161 gcgggtttcg ccgcttttgc ctgctccagt tcctgagtga tttgtttgct atcgggggcc
   146221 gtcgcggcgt acgcccccca actgaggcac caggccatca gaaaagtgat aatcaggcgc
   146281 acgtcagcgt ttcctttgat ggattagacc tggtcttttt tgtcgtcaac caatgggctg
   146341 gcgtcgtgtt ctgcttcgat ctcttcagca ggaagcgggg caggttcagc gtctggcgta
   146401 acaaaggttt cggtagatac tgccagcggc tggccaattt tcgtgacaga caggctttcc
   146461 agttgctcaa ccagattcac tttacccggt gcaaacaggt tgataacggt ggaaccgagt
   146521 ttaaagcgac ccatttcctg gcctttcagc agtgccacag aaccgtcgtt ttccccggca
   146581 ggccaggtcc agcgcttgat gataccttcg cgcggcggcg taatggtgcc cgcccagacc
   146641 gtctcaatgc tgccaacaat cgtcgctccg accagaatct gcgccattgg gccaaattcg
   146701 gtatcgaaaa ggcaaatcac gcgttcgtta cgggcaaaca gattcggcac gttctgagcg
   146761 gtgagatggt taacggagaa gagatcgccc ggcacgtaga tcatctcacg cagaataccg
   146821 ttgcacggca tgtgtacgcg gtggtagtca cgcggggaga ggtaagtggt cacaaacgta
   146881 ccgttgcgga acaggtccgc catcagatag ttgcctgcca gcaaggcttc gaggctgtag
   146941 ttgtggcctt tggcttgcag gattttatct tcttcgattt tacccaactg gctgataacg
   147001 ccatcggcag gcatgaccag gacattcgga tcggtatcga ttgggcgtac ttcgtcacgc
   147061 agcggacgga caaagaattc gttaaaggtg cggtagctgg cggtgtccgg cttttgcgcc
   147121 tctttcatgt cgaccttgta gtatttaacg aacagatcga taaccagttt tgtcagccat
   147181 cctgcccgct tgcttgcgcc ccaacccgcc aggcgagtaa gccatagttt cggcagaatg
   147241 tactgtagcg aaagtttaaa tgaatttaac aaggtagcct ccaggccatt gttttgtcgt
   147301 tcctgatccg gcctacatgc cggatcctga aaaaaagggg acgattctaa cgacggttag
   147361 cttaattgtc agtcatccgt atcagaaaag tttttacgcg tttttacctg cgccatgctt
   147421 tccagaatac ggtgatagtt ttcgaaacgg gtttccgcga ttttcccttc ctcaaccgct
   147481 tcccggatag cgcagcccgg atcggtatcg tgtttgcaat cgcgatattt acacagacct
   147541 aagtagtcat ggaattcgac aaagccctga gtgatttgtt ccggctccag gtgccagagg
   147601 ccgaactcac gcacaccagg ggaatcaatc acatcaccgc cgtgcgggaa gtgatacagc
   147661 cgagcggcgg tcgtggtgtg ctgaccgagc cccgagttgt cagagacatc gttggtcagg
   147721 atctcttttt gcagccccag caacgcattc agcaggctgg atttgccgac gccagactgc
   147781 ccggcaaaaa tgctgatgcg cccggtcaac gcctcttcca gcggttttag cccatcctga
   147841 gtatggctgg aaaccatcaa tacgcgataa ccgatattgc ggtagatatc catctgctcg
   147901 ttgacgaacg ccatgccttc gtcgtccagc aggtctatct tgttgagcac aataatcggc
   147961 tcaatctgca aggtttcgca ggccaccagg taacggtcga taatattgag cgacagctcc
   148021 ggcaaaatgg cggagacaat gacaatctgg tcgatgttgg cggcaatagg ttttacgccg
   148081 tcgtagaaat ccggacgcgt caacaccgag gtacgctcat gcaccgcttc cacgatccct
   148141 ttgacattta cgccttccgc cgccggttta cccggacgcc agactacgcg gtcgccggtt
   148201 accagcgaac ggatggtacg gcgaatattg cagcggtgaa cgtcgccatc ggcggattcc
   148261 acatcagcgt gcataccaaa gcggctgatg acgatacctt catcaggctc gccaaacaga
   148321 ttgtcgtcgt agtcgggctt ctccttagac gttttaagac gacgctggtg attggcgttc
   148381 acgcggcgct gctggccttt ggagagttta tttttactca atcgtacaga ctcctggtcg
   148441 cccctgatgg gcaaaacatc tatgatacac gcaattgtgg atcaatatag tcactgtgaa
   148501 tgggtggaaa atagcatgag tgccaatgaa aacaacctga tttggatcga tcttgagatg
   148561 accggtctgg atcccgagcg cgatcgcatt attgagattg ccacgctggt gaccgatgcc
   148621 aacctgaata ttctggcaga agggccgacc attgcagtac accagtctga tgaacagctg
   148681 gcgctgatgg atgactggaa cgtgcgcacc cataccgcca gcgggctggt agagcgcgtg
   148741 aaagcgagca cgatgggcga tcgggaagct gaactggcaa cgctcgaatt tttaaaacag
   148801 tgggtgcctg cgggaaaatc gccgatttgc ggtaacagca tcggtcagga ccgtcgtttc
   148861 ctgtttaaat acatgccgga gctggaagcc tacttccact accgttatct cgatgtcagc
   148921 accctgaaag agctggcgcg ccgctggaag ccggaaattc tggatggttt taccaagcag
   148981 gggacgcatc aggcgatgga tgatatcc
//
LOCUS       DAMZET010000002        95135 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.002, whole genome shotgun sequence.
ACCESSION   DAMZET010000002 DAMZET010000000
VERSION     DAMZET010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 95135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.002"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(4..1668)
                     /gene="glnS"
                     /locus_tag="POB34_000135"
     CDS             complement(4..1668)
                     /gene="glnS"
                     /locus_tag="POB34_000135"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HDI5917161.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1936..3882)
                     /gene="nagE"
                     /locus_tag="POB34_000136"
     CDS             complement(1936..3882)
                     /gene="nagE"
                     /locus_tag="POB34_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HDI5917162.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVRAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4215..5015
                     /gene="nagB"
                     /locus_tag="POB34_000137"
     CDS             4215..5015
                     /gene="nagB"
                     /locus_tag="POB34_000137"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDI5917163.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5075..6223
                     /gene="nagA"
                     /locus_tag="POB34_000138"
     CDS             5075..6223
                     /gene="nagA"
                     /locus_tag="POB34_000138"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDI5917164.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6232..7452
                     /gene="nagC"
                     /locus_tag="POB34_000139"
                     /gene_synonym="nagR"
     CDS             6232..7452
                     /gene="nagC"
                     /locus_tag="POB34_000139"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HDI5917165.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7500..8252
                     /gene="nagD"
                     /locus_tag="POB34_000140"
     CDS             7500..8252
                     /gene="nagD"
                     /locus_tag="POB34_000140"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HDI5917166.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8649..10313
                     /gene="asnB"
                     /locus_tag="POB34_000141"
     CDS             8649..10313
                     /gene="asnB"
                     /locus_tag="POB34_000141"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HDI5917167.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVTDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10692..10768
                     /locus_tag="POB34_000142"
     tRNA            10692..10768
                     /locus_tag="POB34_000142"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10726..10728,aa:Met,seq:cat)
     gene            10777..10861
                     /locus_tag="POB34_000143"
     tRNA            10777..10861
                     /locus_tag="POB34_000143"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10811..10813,aa:Leu,seq:tag)
     gene            10885..10959
                     /locus_tag="POB34_000144"
     tRNA            10885..10959
                     /locus_tag="POB34_000144"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10917..10919,aa:Gln,seq:ttg)
     gene            10994..11068
                     /locus_tag="POB34_000145"
     tRNA            10994..11068
                     /locus_tag="POB34_000145"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11026..11028,aa:Gln,seq:ttg)
     gene            11084..11160
                     /locus_tag="POB34_000146"
     tRNA            11084..11160
                     /locus_tag="POB34_000146"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11118..11120,aa:Met,seq:cat)
     gene            11209..11283
                     /locus_tag="POB34_000147"
     tRNA            11209..11283
                     /locus_tag="POB34_000147"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11241..11243,aa:Gln,seq:ctg)
     gene            11321..11395
                     /locus_tag="POB34_000148"
     tRNA            11321..11395
                     /locus_tag="POB34_000148"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11353..11355,aa:Gln,seq:ctg)
     gene            complement(11549..12724)
                     /gene="ubiF"
                     /locus_tag="POB34_000149"
     CDS             complement(11549..12724)
                     /gene="ubiF"
                     /locus_tag="POB34_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HDI5917168.1"
                     /translation="MTNQPTEIAIVGVGMVGGALALGLAQHGFTVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRHDDLQELELKDGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPILKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFMRNLGLMAAERAGVLKRQALKYALGL"
     gene            12870..14294
                     /gene="miaB"
                     /locus_tag="POB34_000150"
     CDS             12870..14294
                     /gene="miaB"
                     /locus_tag="POB34_000150"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDI5917169.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14447..15487
                     /locus_tag="POB34_000151"
     CDS             14447..15487
                     /locus_tag="POB34_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDI5917170.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15484..15951
                     /gene="ybeY"
                     /locus_tag="POB34_000152"
     CDS             15484..15951
                     /gene="ybeY"
                     /locus_tag="POB34_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDI5917171.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPHFQKESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            16041..16919
                     /gene="corC"
                     /locus_tag="POB34_000153"
     CDS             16041..16919
                     /gene="corC"
                     /locus_tag="POB34_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HDI5917172.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16944..18482
                     /gene="lnt"
                     /locus_tag="POB34_000154"
     CDS             16944..18482
                     /gene="lnt"
                     /locus_tag="POB34_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HDI5917173.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPSLWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(18560..20437)
                     /locus_tag="POB34_000155"
     CDS             complement(18560..20437)
                     /locus_tag="POB34_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDI5917174.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGAGTYHKNQR
                     RVFSLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSNKPGAWV
                     MALSPDEKILLIPGMVRGDIVRINTITHQKESYPASDARGTISAMRFRPENGDVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQLDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            20892..21800
                     /gene="gltI"
                     /locus_tag="POB34_000156"
     CDS             20892..21800
                     /gene="gltI"
                     /locus_tag="POB34_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HDI5917175.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            21970..22710
                     /gene="gltJ"
                     /locus_tag="POB34_000157"
     CDS             21970..22710
                     /gene="gltJ"
                     /locus_tag="POB34_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HDI5917176.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            22710..23384
                     /gene="gltK"
                     /locus_tag="POB34_000158"
     CDS             22710..23384
                     /gene="gltK"
                     /locus_tag="POB34_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HDI5917177.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            23384..24109
                     /gene="gltL"
                     /locus_tag="POB34_000159"
     CDS             23384..24109
                     /gene="gltL"
                     /locus_tag="POB34_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HDI5917178.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            24227..25162
                     /gene="rihA"
                     /locus_tag="POB34_000160"
     CDS             24227..25162
                     /gene="rihA"
                     /locus_tag="POB34_000160"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HDI5917179.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            25246..26916
                     /gene="hscC"
                     /locus_tag="POB34_000161"
                     /pseudo
     CDS             25246..26916
                     /gene="hscC"
                     /locus_tag="POB34_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(26976..28427)
                     /gene="djlC"
                     /locus_tag="POB34_000162"
     CDS             complement(26976..28427)
                     /gene="djlC"
                     /locus_tag="POB34_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HDI5917180.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(28424..29131)
                     /locus_tag="POB34_000163"
     CDS             complement(28424..29131)
                     /locus_tag="POB34_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDI5917181.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            29233..29787
                     /locus_tag="POB34_000164"
     CDS             29233..29787
                     /locus_tag="POB34_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDI5917182.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(29797..31224)
                     /locus_tag="POB34_000165"
     CDS             complement(29797..31224)
                     /locus_tag="POB34_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HDI5917183.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYAQSWREAEPDNEDAPYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALMLQWCRQHRVDYYPLLVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQACQPT
                     EESLMPIVSRLDAENRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(31221..31928)
                     /locus_tag="POB34_000166"
     CDS             complement(31221..31928)
                     /locus_tag="POB34_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDI5917184.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
     gene            32092..33069
                     /locus_tag="POB34_000167"
     CDS             32092..33069
                     /locus_tag="POB34_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HDI5917185.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLEQKYTEDKSRHKDNEHAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNETLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQGSVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIREIEDLL"
     gene            complement(33139..33621)
                     /locus_tag="POB34_000168"
     CDS             complement(33139..33621)
                     /locus_tag="POB34_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HDI5917186.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            33856..36438
                     /gene="leuS"
                     /locus_tag="POB34_000169"
     CDS             33856..36438
                     /gene="leuS"
                     /locus_tag="POB34_000169"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDI5917187.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            36453..37034
                     /gene="lptE"
                     /locus_tag="POB34_000170"
     CDS             36453..37034
                     /gene="lptE"
                     /locus_tag="POB34_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HDI5917188.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            37034..38065
                     /gene="holA"
                     /locus_tag="POB34_000171"
     CDS             37034..38065
                     /gene="holA"
                     /locus_tag="POB34_000171"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDI5917189.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            38067..38708
                     /gene="nadD"
                     /locus_tag="POB34_000172"
     CDS             38067..38708
                     /gene="nadD"
                     /locus_tag="POB34_000172"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDI5917190.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDNLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            38732..39343
                     /locus_tag="POB34_000173"
     CDS             38732..39343
                     /locus_tag="POB34_000173"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HDI5917191.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARLVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSTIDINQKFATLRVLNSRAIGVENA"
     gene            39603..39920
                     /gene="rsfS"
                     /locus_tag="POB34_000174"
                     /gene_synonym="rsfA"
     CDS             39603..39920
                     /gene="rsfS"
                     /locus_tag="POB34_000174"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDI5917192.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            39924..40391
                     /gene="rlmH"
                     /locus_tag="POB34_000175"
     CDS             39924..40391
                     /gene="rlmH"
                     /locus_tag="POB34_000175"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDI5917193.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            40422..42323
                     /gene="mrdA"
                     /locus_tag="POB34_000176"
     CDS             40422..42323
                     /gene="mrdA"
                     /locus_tag="POB34_000176"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HDI5917194.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            42326..43438
                     /gene="mrdB"
                     /locus_tag="POB34_000177"
                     /gene_synonym="rodA"
     CDS             42326..43438
                     /gene="mrdB"
                     /locus_tag="POB34_000177"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HDI5917195.1"
                     /translation="MTDKPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            43449..44537
                     /gene="rlpA"
                     /locus_tag="POB34_000178"
     CDS             43449..44537
                     /gene="rlpA"
                     /locus_tag="POB34_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HDI5917196.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGASSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLESSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            44677..45888
                     /gene="dacA"
                     /locus_tag="POB34_000179"
     CDS             44677..45888
                     /gene="dacA"
                     /locus_tag="POB34_000179"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HDI5917197.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            45999..46262
                     /locus_tag="POB34_000180"
     CDS             45999..46262
                     /locus_tag="POB34_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HDI5917198.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            46363..47004
                     /gene="lipB"
                     /locus_tag="POB34_000181"
     CDS             46363..47004
                     /gene="lipB"
                     /locus_tag="POB34_000181"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HDI5917199.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            47263..48216
                     /locus_tag="POB34_000182"
     CDS             47263..48216
                     /locus_tag="POB34_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDI5917200.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQQTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            48425..49390
                     /gene="lipA"
                     /locus_tag="POB34_000183"
     CDS             48425..49390
                     /gene="lipA"
                     /locus_tag="POB34_000183"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDI5917201.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(49491..49694)
                     /gene="tatE"
                     /locus_tag="POB34_000184"
     CDS             complement(49491..49694)
                     /gene="tatE"
                     /locus_tag="POB34_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HDI5917202.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(49823..50611)
                     /locus_tag="POB34_000185"
     CDS             complement(49823..50611)
                     /locus_tag="POB34_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HDI5917203.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            50704..51087
                     /gene="crcB"
                     /locus_tag="POB34_000186"
     CDS             50704..51087
                     /gene="crcB"
                     /locus_tag="POB34_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HDI5917204.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLTANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(51141..51350)
                     /gene="cspE"
                     /locus_tag="POB34_000187"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(51141..51350)
                     /gene="cspE"
                     /locus_tag="POB34_000187"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDI5917205.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(51525..52085)
                     /gene="pagP"
                     /locus_tag="POB34_000188"
     CDS             complement(51525..52085)
                     /gene="pagP"
                     /locus_tag="POB34_000188"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HDI5917206.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            52674..54059
                     /gene="dcuC"
                     /locus_tag="POB34_000189"
     CDS             52674..54059
                     /gene="dcuC"
                     /locus_tag="POB34_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDI5917207.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(54100..54780)
                     /gene="dpiA"
                     /locus_tag="POB34_000190"
                     /gene_synonym="citB"
     CDS             complement(54100..54780)
                     /gene="dpiA"
                     /locus_tag="POB34_000190"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HDI5917208.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(54749..56407)
                     /gene="dpiB"
                     /locus_tag="POB34_000191"
     CDS             complement(54749..56407)
                     /gene="dpiB"
                     /locus_tag="POB34_000191"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HDI5917209.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            56787..57845
                     /gene="citC"
                     /locus_tag="POB34_000192"
     CDS             56787..57845
                     /gene="citC"
                     /locus_tag="POB34_000192"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HDI5917210.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            57860..58156
                     /gene="citD"
                     /locus_tag="POB34_000193"
     CDS             57860..58156
                     /gene="citD"
                     /locus_tag="POB34_000193"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HDI5917211.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            58153..59061
                     /gene="citE"
                     /locus_tag="POB34_000194"
     CDS             58153..59061
                     /gene="citE"
                     /locus_tag="POB34_000194"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HDI5917212.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            59072..60604
                     /gene="citF"
                     /locus_tag="POB34_000195"
     CDS             59072..60604
                     /gene="citF"
                     /locus_tag="POB34_000195"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HDI5917213.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            60608..61159
                     /gene="citX"
                     /locus_tag="POB34_000196"
     CDS             60608..61159
                     /gene="citX"
                     /locus_tag="POB34_000196"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HDI5917214.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            61134..62012
                     /gene="citG"
                     /locus_tag="POB34_000197"
     CDS             61134..62012
                     /gene="citG"
                     /locus_tag="POB34_000197"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HDI5917215.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            62063..63526
                     /gene="citT"
                     /locus_tag="POB34_000198"
     CDS             62063..63526
                     /gene="citT"
                     /locus_tag="POB34_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HDI5917216.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            63640..64446
                     /gene="rna"
                     /locus_tag="POB34_000199"
     CDS             63640..64446
                     /gene="rna"
                     /locus_tag="POB34_000199"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HDI5917217.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            64676..65086
                     /gene="rnk"
                     /locus_tag="POB34_000200"
     CDS             64676..65086
                     /gene="rnk"
                     /locus_tag="POB34_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HDI5917218.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(65317..66555)
                     /locus_tag="POB34_000201"
     CDS             complement(65317..66555)
                     /locus_tag="POB34_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HDI5917219.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            66776..67204
                     /gene="uspG"
                     /locus_tag="POB34_000202"
     CDS             66776..67204
                     /gene="uspG"
                     /locus_tag="POB34_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HDI5917220.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(67325..68890)
                     /gene="ahpF"
                     /locus_tag="POB34_000203"
     CDS             complement(67325..68890)
                     /gene="ahpF"
                     /locus_tag="POB34_000203"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDI5917221.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(69061..69624)
                     /gene="ahpC"
                     /locus_tag="POB34_000204"
     CDS             complement(69061..69624)
                     /gene="ahpC"
                     /locus_tag="POB34_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HDI5917222.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            69995..70741
                     /gene="dsbG"
                     /locus_tag="POB34_000205"
     CDS             69995..70741
                     /gene="dsbG"
                     /locus_tag="POB34_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HDI5917223.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            70950..71852
                     /locus_tag="POB34_000206"
     CDS             70950..71852
                     /locus_tag="POB34_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI5917224.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIIAEEFTQLISKSAGVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            72000..73220
                     /locus_tag="POB34_000207"
     CDS             72000..73220
                     /locus_tag="POB34_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDI5917225.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            73193..73822
                     /locus_tag="POB34_000208"
     CDS             73193..73822
                     /locus_tag="POB34_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDI5917226.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(73823..74983)
                     /gene="ybdL"
                     /locus_tag="POB34_000209"
     CDS             complement(73823..74983)
                     /gene="ybdL"
                     /locus_tag="POB34_000209"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HDI5917227.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            75092..76180
                     /gene="hcxA"
                     /locus_tag="POB34_000210"
     CDS             75092..76180
                     /gene="hcxA"
                     /locus_tag="POB34_000210"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HDI5917228.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAEIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNHAEIDKVITHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(76190..76387)
                     /locus_tag="POB34_000211"
     CDS             complement(76190..76387)
                     /locus_tag="POB34_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDI5917229.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(76569..78674)
                     /gene="cstA"
                     /locus_tag="POB34_000212"
     CDS             complement(76569..78674)
                     /gene="cstA"
                     /locus_tag="POB34_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HDI5917230.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(78855..79268)
                     /gene="entH"
                     /locus_tag="POB34_000213"
     CDS             complement(78855..79268)
                     /gene="entH"
                     /locus_tag="POB34_000213"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HDI5917231.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(79271..80017)
                     /gene="entA"
                     /locus_tag="POB34_000214"
     CDS             complement(79271..80017)
                     /gene="entA"
                     /locus_tag="POB34_000214"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HDI5917232.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(80017..80874)
                     /gene="entB"
                     /locus_tag="POB34_000215"
     CDS             complement(80017..80874)
                     /gene="entB"
                     /locus_tag="POB34_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HDI5917233.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(80888..82498)
                     /gene="entE"
                     /locus_tag="POB34_000216"
     CDS             complement(80888..82498)
                     /gene="entE"
                     /locus_tag="POB34_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HDI5917234.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(82508..83683)
                     /gene="entC"
                     /locus_tag="POB34_000217"
     CDS             complement(82508..83683)
                     /gene="entC"
                     /locus_tag="POB34_000217"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HDI5917235.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            84058..85014
                     /gene="fepB"
                     /locus_tag="POB34_000218"
     CDS             84058..85014
                     /gene="fepB"
                     /locus_tag="POB34_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HDI5917236.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSATQVLERLKALF"
     gene            complement(85018..86268)
                     /gene="entS"
                     /locus_tag="POB34_000219"
     CDS             complement(85018..86268)
                     /gene="entS"
                     /locus_tag="POB34_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HDI5917237.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAIMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            86379..87383
                     /gene="fepD"
                     /locus_tag="POB34_000220"
     CDS             86379..87383
                     /gene="fepD"
                     /locus_tag="POB34_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HDI5917238.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            87380..88372
                     /gene="fepG"
                     /locus_tag="POB34_000221"
     CDS             87380..88372
                     /gene="fepG"
                     /locus_tag="POB34_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HDI5917239.1"
                     /translation="MIYVSRRLIITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            88369..89184
                     /gene="fepC"
                     /locus_tag="POB34_000222"
     CDS             88369..89184
                     /gene="fepC"
                     /locus_tag="POB34_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HDI5917240.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(89181..90314)
                     /gene="fepE"
                     /locus_tag="POB34_000223"
     CDS             complement(89181..90314)
                     /gene="fepE"
                     /locus_tag="POB34_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HDI5917241.1"
                     /translation="MSSLNIKQGSDAHFPEYPLASPSNNEIDLLNLIAVLWRAKKTVM
                     AVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISTLVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKAHVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGGVLLRYAMASRKQDAMMADHLV"
     gene            complement(90530..94411)
                     /gene="entF"
                     /locus_tag="POB34_000224"
     CDS             complement(90530..94411)
                     /gene="entF"
                     /locus_tag="POB34_000224"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HDI5917242.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSELPSAWSVAHYVELTGEVDSPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALTFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPDVQAQTHTLATGPVNDLELALFPDVHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEAKGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(94408..94626)
                     /gene="ybdZ"
                     /locus_tag="POB34_000225"
     CDS             complement(94408..94626)
                     /gene="ybdZ"
                     /locus_tag="POB34_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HDI5917243.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEAHWRTLTPTNFTQLQEAQ"
     gene            complement(94629..>95135)
                     /gene="fes"
                     /locus_tag="POB34_000226"
     CDS             complement(94629..>95135)
                     /gene="fes"
                     /locus_tag="POB34_000226"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HDI5917244.1"
                     /translation="DAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRGDRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
BASE COUNT        23390 a        25046 c        23810 g        22889 t
ORIGIN      
        1 aaattactcg cctactttcg cccaggtatc acgcagccca acggtgcggt taaataccgg
       61 tttttccgcc gtagaatggc ggctatcgag gcagaagtaa ccttcacgct caaactggaa
      121 tgctttaccc gcaaccgcat ctttcagcga cggttcagca aagccctgtt tgatcaccag
      181 cgattccggg ttaatcaccg acaggaaatc atccgcagca cctgggttag gcacgctgaa
      241 cagacggtca tacaaacgga tttcaaccgg cagcgcatgt gccgcgctca cccagtgaat
      301 aacacctttg actttacgac catctgccgg atctttgctt aaggtatcgg cgtcataagt
      361 acagaagatg gtggtgatat taccttcggc atctttctcg acgcgttctg ccttaatcac
      421 ataagcatta cgcagacgca cttctttacc cagcaccaga cgtttgtact gcttgttagc
      481 ttcttcgcgg aaatcggcgc gatcaatcca aatctcaccg ctaaacggca cctgacggct
      541 gcccatttcc ggtttgttcg gatggttcgg catggtaacc atttcgcctt cgccctgata
      601 gttttcgata accagtttca ccggatcgat aaccgccatt gcgcgcggcg cattttcgtt
      661 gagatcttca cggatgcagg attccagcga cgccatctca atggtgttgt cctgcttggt
      721 cacgccgatg cgtttgcaga actcacgaat agaagccgca gtgtaaccac gacgacgcag
      781 accggaaatg gtcggcatac gcgggtcatc ccagccttca acgtgcttgt cggtcaccag
      841 caggttcaac ttacgcttgg acatcacggt gtattccaga ttcaggcgcg agaactcata
      901 ctggcgcggg tgaacaggaa tcgtgatgtt gtccagtacc cagtcgtaca gacgacggtt
      961 gtcctggaac tcaagcgtac acagagagtg cgtaatacct tccaaggcat cgctgatgca
     1021 gtgggtgaag tcgtacatcg ggtagatgca ccacttgttg ccagtctggt ggtgttcagc
     1081 aaatttaata cggtacagca ccggatcgcg catcacgata aacggtgaag ccatgtcgat
     1141 tttcgcacgc aggcaggctt taccttcttc aaaaccaccg gcacgcattt tttcgaacag
     1201 cgccaggttc tcttcaacgc tgcggtcgcg gtacgggctg tttttacccg gttgcgtcag
     1261 ggtgccgcgg tattcgcgga tctgttccgg cgtcagttca tcaacgtacg ccaggccttt
     1321 attgatcagt tcgatcgcat aggcgtggag ctgatcaaaa taatcggagg agtaacggac
     1381 gttaccagac cagtgaaaac ctaaccactc tacgtcgttt ttgatcgact caacatactc
     1441 gatatcttct tttaccgggt tagtgtcgtc gaaacgcagg ttgcactggc ctttatagtc
     1501 ctgggcgatc ccgaagttca ggcagataga tttcgcatgg ccaatatgca gatagccatt
     1561 cggctccggc gggaaacggg tgtgtactgt ggtgtgctta ccactggcca gatcttcatc
     1621 gatgatctga cggataaagt tagtcgggcg ggcttctgcc tcactcatcg tggattcctc
     1681 aaagcgtaaa caacgtataa cggcgtatga tcttataagc gggacaggct gacaactgtt
     1741 agttttttaa aattgataat ctaacaagga ttatggggat gattgatgaa aaaaaaagcg
     1801 gcagagaaga tctccaccgc ttttgccagt cctgcgtgat tgtcggatgc aacgctaaag
     1861 cgtcttatcc aacctggact gagcttttgt aggcctgata agacgcgtca agcgtcgcat
     1921 caggcatgaa gcagattact ttttgatttc atacagcggt gtttgacccg ccacaacatg
     1981 gccctgagct ttaatgatca agccactgaa atcgtcgata ttgctgcaaa ccaccggact
     2041 aatcatcgag cgggcgttag cgttcaggta atccagatcc atttccagaa tcggttgccc
     2101 tgctcttacc tgcgcgccct cttccaccag acgtttaaag cctttacctt ccagcgctac
     2161 ggtgtcgata cccatgtgga cgacgatctc cgcgcctttt tcggtttcca gacagaacgc
     2221 gtggttggtg ttgaagattt tcacgattgt ccctgcggct ggtgatacga cgattttatc
     2281 tgtcggtttc accgccacac cgtcacccac cgctttgctg gcgaatgctt cgtcaggaac
     2341 ctgatccagt gccacgacat caccggtaat cggcgatacc agctccgcga tagataccgc
     2401 gtttggtaca gcctgcggtt ttgctacagg ggcggcagtt gccggagttg cttcagctga
     2461 cgcagctgct accgggccac gggcaacgac tttcttcatc gcatcgccaa tggattctgc
     2521 tttcgcgcca acaatcacct gaatggtctg tttgttcagt tttactacac cagaagcacc
     2581 cagacgttta cacatcgcat cgttgacgcg agctgagtca gccacggtaa ggcgcagacg
     2641 ggtaatacag gcgtcaatcg ctttcaggtt gtcagtaccg ccaaccgcag caatatagtt
     2701 ggttgccagt tgagtcagac cttcttcagt attgctgtta gcctcttcag ttacgatctc
     2761 gtcttcttta tcttcacgac ccggcgtttt cagattgaac atgcggataa ccaaactgaa
     2821 caccacgaag tagatagcga agaagacaac acccataacc agcagcatcc agacgttctg
     2881 gctggcggcc ggcaggttat acatcaacgc gtagtcgata gcacccgcag agaaagagaa
     2941 gccagcatgg atacccagca gcgttgccac aaacaggctg ataccggtca gcagtgcgtg
     3001 caggaggtac agcagcggtg caaggaacat gaacaggaat tccagcggct cagtcacacc
     3061 ggtcaggaac gcagtaacag caacagaaag cagcatcccg ccaaccatcg gacgacgctc
     3121 tttcggtgct gcgaagtaca tcgccagcgc cgcacccggc agaccgaaca tcatgatcgg
     3181 gaagaagccg gacatgaaca tccccgcggt gccgtcacca gcgtagaaac ggttgatgtc
     3241 gccgtggaaa accgtacccg ccgcgttggt gaattcacca atctggaacc aggcgatggt
     3301 gttcagcacc tgatgcagac cggttgggat cagcagacgg ttgatgaaac caaagatacc
     3361 ggaacccagc gcgcccgcag aaacgatcca ctcgccgcct gcatggatag cgtgctgtac
     3421 cggcggccag acgtaaccaa aaatggccgc cagcaccaga cagaagaagc cggtggcgat
     3481 cggcacaaag cgtttgccgc cgaagaagct caggaagtcc ggcagtttaa tatcggacca
     3541 acggttatag gctgcgccac caaccagacc ggtaatgata cccgccagta cacccatgtt
     3601 aatttctggg ttgatggtca ccatcgcttt ggttaacaca aagtaaccta ccgcacccgc
     3661 cagcgccgcc gcacctgcgc tgtctttcga ccagctggat gccacaccga tggcgaagat
     3721 taatgcgagg ttatcaaaaa tcgcaccgcc cgcctgggca ataaacgcaa cgttaagtaa
     3781 atctggctga ccgaatcgca gcaacagtgc cgccaccggc agcaccgcga tagggagctg
     3841 taacgcccta ccgagtcgct ggaaaaaacc taaaatattc atcttattcc ccctacgaga
     3901 accctatttg gctcgtttaa agccgtattt ttattttgct gcaaattgta ctgccgatgt
     3961 tctgtaatca gattgttaga tcatctgcta cagagtgtgt gaaaatttaa ttcgtatcgc
     4021 aaattaaatg cgtgtctttt gtgagttttg tcaccaaata tcgttatcat cactcccttt
     4081 tactggctaa accagaaaac ttattttatc attcaaaaaa tcaggtcgga ttgacgcctg
     4141 tctgcgcaaa tccaggttac gcttaaagat gcctaatccg ccaacggctt acattttact
     4201 tattgaggtg aataatgaga ctgatccccc tgactaccgc tgaacaggtc ggcaaatggg
     4261 ctgctcgcca tatcgtcaat cgtatcaatg cgttcaaacc gactgccgat cgtccgtttg
     4321 tactgggcct gccgactggc ggcacgccga tgaccaccta taaagcgtta gtcgaaatgc
     4381 ataaagcagg ccaggtcagc tttaagcacg ttgtcacctt caacatggac gaatatgtcg
     4441 gtctgccgaa agagcatccg gaaagctact acagctttat gcaccgtaat ttcttcgatc
     4501 acgttgatat tccagcagaa aacatcaacc ttctcaacgg caatgccccg gatatcgacg
     4561 ccgagtgccg ccagtatgaa gaaaaaatcc gttcttacgg aaaaattcat ctgtttatgg
     4621 gcggtgtagg taacgacggt catattgcat ttaacgaacc ggcgtcttct ctggcttctc
     4681 gtactcgtat caaaaccctg actcatgaca ctcgcgtcgc gaactctcgt ttctttgata
     4741 acgatgtcaa tcaggtgcca aaatatgccc tgactgtcgg tgttggtaca ttgctggatg
     4801 ccgaagaagt gatgattctg gtgctgggta gccagaaagc actggcactg caggctgcag
     4861 ttgaaggttg cgtgaaccac atgtggacca tcagctgtct gcaactgcat ccaaaagcga
     4921 tcatggtgtg cgatgaacct tccaccatgg agctgaaagt taagacttta agatatttca
     4981 atgaattaga agcagaaaat atcaaaggtc tgtaattgtt atccctgccc tggctccttg
     5041 ctcagggcaa tattttttaa aatcgggggt cagaatgtat gcattaaccc agggccggat
     5101 ctttaccggc cacgaatttc ttgatgacca cgcggttgtt atcgctgatg gcctgattaa
     5161 aagcgtctgt ccggtagcgg aactgccgcc agagatcgaa caacgttcac tgaacggggc
     5221 cattctctcc cccggtttta tcgatgtgca gttaaacggc tgcggcggcg tacagtttaa
     5281 cgacaccgct gaagcggtca gcgtggaaac gctggaaatc atgcagaaag ccaatgagaa
     5341 atcaggctgt actaactatc tgccgacgct tatcaccacc agcgatgagc tgatgaaaca
     5401 gggcgtgcgc gttatgcgcg agtacctggc aaaacatccc aatcaggcgt taggtctgca
     5461 tctggaaggt ccgtggctga atctggtaaa aaaaggcacc cataatccga attttgtgcg
     5521 taagcctgat gccgcgctgg tcgatttcct gtgtgaaaac gccgacgtca ttaccaaagt
     5581 gaccctggca ccggaaatgg ttcctgcgga agtcatcagc aaactggcaa atgccgggat
     5641 tgtggtttct gccggtcact ccaacgcgac gttgaaagaa gcgaaagccg gtttccgcgc
     5701 ggggattacc tttgccaccc atctgtacaa tgcgatgccg tatattaccg gtcgtgaacc
     5761 gggcctggcg ggcgcgatcc tcgacgaagc tgacatttat tgcggtatta tcgctgatgg
     5821 cctgcatgtt gattacgcca acattcgtaa cgctaaacgc cttaaaggcg acaaactgtg
     5881 tctggttacc gacgccaccg cgccagcagg tgccaacatt gaacagttca tttttgcggg
     5941 taaaacaata tactaccgta acggactttg tgtggatgag aacggtacgt taagcggttc
     6001 atccttaacc atgattgaag gcgtgcgtaa tctggtcgaa cattgcggta tcgcactgga
     6061 tgaagtgctg cgtatggcga cgctctatcc ggcgcgtgcg attggcgttg agaaacgtct
     6121 cggcacactc gccgcaggta aagtagccaa cctgaccgca ttcacacctg attttaaaat
     6181 caccaagacc atcgttaacg gtaacgaggt cgtaactcaa taagagaaag tatgacacca
     6241 ggcggacaag ctcagatagg taatgttgat ctcgtaaaac agcttaacag cgcggcggtt
     6301 tatcgcctga ttgaccagta cgggccaatc tcgcggattc agattgccga gcaaagccag
     6361 cttgcccccg ccagcgtaac caaaattacg cgtcagctta tcgaacgcgg gctgatcaaa
     6421 gaagttgatc agcaggcctc caccgggggc cgccgcgcta tctccatcgt caccgaaacc
     6481 cgcaatttcc acgcaatcgg cgtacggctt ggtcgtcatg acgccaccat cactctgttt
     6541 gatctcagca gcaaagtgct ggcagaagaa cattacccgc tgccggaacg tacccagcag
     6601 acgctggaac atgccctgct gaatgccatt gctcagttta ttgatagcta ccaacgcaag
     6661 ctgcgcgagc tgatcgccat ttcggtgatc ctgccagggc ttgttgaccc ggacagcggc
     6721 aaaattcatt acatgccgca tattcaggta gaaaactggg ggctggtaga agcactggaa
     6781 gaacgtttta aagtgacctg tttcgttggt cacgatatcc gtagtctggc gctggcggag
     6841 cactacttcg gtgcaagtca ggattgcgaa gactccattc tggtgcgtgt ccatcgcgga
     6901 accggggccg ggattatctc taacgggcgc atttttattg gccgcaacgg caacgtcggt
     6961 gaaattggcc atattcaggt cgaaccgctg ggtgaacgct gccactgcgg caactttggc
     7021 tgcctggaaa ctatcgccgc caacgctgcc attgaacaac gggtgttgaa tctgttaaag
     7081 cagggctacc agagccgtgt gccactggac gactgcacca tcaaaactat ctgcaaagcc
     7141 gcgaacaaag gcgatagcct ggcctcggaa gtgattgagt atgtcggtcg tcatctgggc
     7201 aaaaccatcg ccattgctat caacttattt aatccgcaaa aaattgttat cgctggtgaa
     7261 atcaccgaag ccgataaagt gctgctccct gctattgaaa gctgcattaa tacccaggcg
     7321 ctgaaggcgt ttcgcactaa tctgccggtg gtacgttctg agctggatca ccgctcggca
     7381 atcggcgctt ttgcgctggt aaaacgcgcc atgctcaacg gtattttgct ccagcatttg
     7441 ctggaaaatt aatgtgcttt tatagtggcg cttattgttg tcaatattct gggtagtcca
     7501 tgaccattaa aaatgtaatt tgcgatatcg acggcgtgct gatgcacgat aacgtcgccg
     7561 taccgggtgc agcggaattt ttgcacggga ttatggataa aggcctgccg ctggtgttgc
     7621 tgaccaacta tccttcgcag accgggcagg atctggcgaa ccgctttgcc actgccggtg
     7681 tcgatgtacc tgacagcgtg ttttatacct ctgcgatggc gaccgccgat ttcctgcgtc
     7741 gccaggaagg caagaaagcg tatgtggtgg gcgaaggcgc actgattcat gaactgtaca
     7801 aagccggttt cactattacc gatgtgaacc ctgattttgt gattgttggc gaaacgcgtt
     7861 cctacaactg ggacatgatg cataaagcgg cctatttcgt cgctaacggt gcacgtttta
     7921 tcgccaccaa cccggacacc cacgggcgcg gtttttatcc cgcctgtggc gcgttgtgtg
     7981 cagggatcga aaaaatctcc gggcgtaaac cgttctatgt tggtaagccg agtccgtgga
     8041 tcatccgcgc ggcattaaac aaaatgcagg cgcattcgga agaaacggtg attgtcggcg
     8101 ataacctgcg taccgatatt ctggccggct tccaggcagg tctggagacg attctggtgc
     8161 tttctggtgt ttcgtcgctc gacgatatcg acagtatgcc tttccgcccc agctggattt
     8221 acccgtcagt cgctgaaatc gacgttatct gaatagcaac cacgtctcag gacgtggttg
     8281 gctctgatga caaacgccaa actgcctgat gcgctacgct tatcaggcct acgcagctcc
     8341 tgcaatatat tgaattttca tgcttttgta ggccggataa ggcgttcacg ccgcatccgg
     8401 catgaacaaa tcgcactttg tcagcaattt ggcccccgta aaatataaac attcaataaa
     8461 acccgctaaa aattatcgat tcatcaataa atatatcctc acaccagacc ttttttcacc
     8521 aatcgacaat caccattacg ttttttattt tttcaccgcc aaatcgcttg cgcacagtgc
     8581 tgctttgcgg cattttttta aacaagcaaa cacaacaagc aacaaataca aggttaacgg
     8641 agaaggttat gtgttcaatt tttggcgtat tcgatatcaa aacagacgca gttgagctgc
     8701 gtaagaaagc cctcgagctg tcacgcctga tgcgtcatcg tggcccggac tggtccggta
     8761 tttatgccag cgataacgcc attctcgccc acgaacgtct gtcaattgtt gacgttaacg
     8821 cgggggcgca acctctctac aaccaacaaa aaacccacgt actggcggta aacggtgaaa
     8881 tctacaacca ccaggcattg cgcgccgaat atggcgatcg ttaccagttc cagaccgggt
     8941 ctgactgtga agtgatcctc gcgctgtatc aggaaaaagg gccggaattt ctcgacgact
     9001 tgcagggcat gtttgccttt gcactgtacg acagcgaaaa agatgcctac ctgattggtc
     9061 gcgaccatct ggggatcatc ccactgtata tggggtatga cgaacacggt cagctgtatg
     9121 tggcctcaga aatgaaagcg ctggtgccag tttgccgcac gattaaagag ttcccggcgg
     9181 ggagctattt gtggagccag gacggcgaaa tccgttctta ctatcatcgc gactggttcg
     9241 actacgatgc ggtgaaagat aacgtgaccg acaaaaacga gctgcgtcag gcactggaag
     9301 attcagttaa aagccatctg atgtctgatg tgccttacgg tgtgctgctt tctggtggtc
     9361 tggattcctc aattatttcc gctatcacca agaaatacgc agcccgtcgc gtggaagatc
     9421 aggaacgctc tgaagcctgg tggccgcagt tacactcctt tgctgtaggt ctgccgggtt
     9481 caccggatct gaaagcagcc caggaagtgg caaaccatct gggcacggtg catcacgaaa
     9541 ttcacttcac tgtacaggaa ggtctggatg ccatccgcga cgtgatttac cacatcgaaa
     9601 cttatgatgt gaccactatt cgcgcttcaa caccgatgta tttaatgtcg cgtaagatca
     9661 aggcgatggg cattaaaatg gtgctgtccg gtgaaggttc tgatgaagtg tttggcggtt
     9721 atctttactt ccacaaagcg ccgaacgcca aagaactgca tgaagagacg gtgcgtaaac
     9781 tgctggccct gcatatgtat gactgcgccc gtgccaacaa agcgatgtcc gcctggggcg
     9841 tggaagcgcg cgttccgttc ctcgataaga aattccttga tgtggcgatg cgtattaacc
     9901 cacaggataa aatgtgcggt aacggcaaaa tggaaaaaca catcctgcgt gaatgttttg
     9961 aagcgtatct gcctgcaagc gtggcctggc ggcagaaaga gcagttctcc gatggagtcg
    10021 gttacagttg gatcgacacc ctgaaagaag tggcggcgca gcaggttacc gatcagcaac
    10081 tggaaactgc ccgcttccgc ttcccataca acacgccaac ctctaaagaa gcgtatctgt
    10141 accgggagat ctttgaagaa ctgttcccgc ttccgagcgc cgctgagtgc gtgcctggcg
    10201 gtccttccgt cgcgtgttct tccgctaaag cgatcgaatg ggatgaagcg ttcaagaaaa
    10261 tggacgatcc atctggtcgt gcggttggtg ttcaccagtc agcatataag taagaaatgc
    10321 gaaacagccc cggtgaaatc cggggctgtt tcagttattg aggtgcttgt tgattctgat
    10381 atgcagtaag gaaatgttcc gggtttgcca catcactgtt tgacaactca tcactgtagg
    10441 ccagtatttc atagattaat ttcccaccac tattgttgaa ttccgaattt catcctgtat
    10501 gacaaaagaa tggctgtagg gtgggaaaaa acacaaagta aacaataatt gacgaatata
    10561 gcgccacgct gttcgcaacc taaccaaaca gtcactttcg agcaattttc cttgaaaaag
    10621 aggttgacgc tgcaaggctc tatacgcata atgcgccccg caacgccgat aaggtatcgc
    10681 gaaaaaaaga tggctacgta gctcagttgg ttagagcaca tcactcataa tgatggggtc
    10741 acaggttcga atcccgtcgt agccaccatc ttttttgcgg gagtggcgaa attggtagac
    10801 gcaccagatt taggttctgg cgccgcaagg tgtgcgagtt caagtctcgc ctcccgcacc
    10861 attcaccaga aagcgttgta cggatggggt atcgccaagc ggtaaggcac cggtttttga
    10921 taccggcatt ccctggttcg aatccaggta ccccagccat cttcttcgag taagcggttc
    10981 accgcccggt tattggggta tcgccaagcg gtaaggcacc ggtttttgat accggcattc
    11041 cctggttcga atccaggtac cccagccatc gaagaaacaa tctggctacg tagctcagtt
    11101 ggttagagca catcactcat aatgatgggg tcacaggttc gaatcccgtc gtagccacca
    11161 aattctgaat gtgtcgaata cgttcggcaa attcaaaaac caatttgttg gggtatcgcc
    11221 aagcggtaag gcaccggatt ctgattccgg cattccgagg ttcgaatcct cgtaccccag
    11281 ccaatttatt caagacgctt acatcgtaag tgcacccagt tggggtatcg ccaagcggta
    11341 aggcaccgga ttctgattcc ggcattccga ggttcgaatc ctcgtacccc agccacatta
    11401 aaaaagctcg cttcggcgag ctttttgctt ttctgcgttc attcaatgtc gaatgcgatg
    11461 ttgacacgtc ttatccttca atgtcggatg cgacgctgcc gcgtcttatc cgacctacgg
    11521 ttggcacgca tccggcaatg ttgtaaggct acaaccctaa cgcatatttc agcgcctgac
    11581 gtttcaacac gccagcacgc tccgccgcca ttaacccgag attacgcata aaacgcagtg
    11641 gtggcagatt attgctgaat ccggcataaa acagatccat accgctttgc ataatgaagt
    11701 tatccgccat gcgccgcatc tggtaacgct tgaggattgg ataactggcc cacgcttcgc
    11761 cgtagctgcg ggcgttgacc agaacatcaa tcagggcatc gacatcacga taaccaagat
    11821 tcaccccctg ccccgccagc ggatggatgg tatgcgcggc atcgcccacc agcgcaagcc
    11881 ctggctgcac atactgcaac gcatggcgac gcgtcagcgg aaacgcacca gcggcaagcg
    11941 gtgtaacgta acccagacgc gacgggaaat gcttcgcgat ttccgcctgt aactgcgcca
    12001 tgttcatatt ctgcaactgg cgaatacgcg ccggagagtc ataccacacc agcgatgccc
    12061 agttatcaaa caacggcaga aacgcacgcg gtccgtccgg agtaaattgc tgccaggtgc
    12121 tgtcgccggg atcgttctca cactgtacgc taatcaacat acacgactgc gcatactgcc
    12181 aggcatgaac gccaataccc gccatctgcc gcacctgcga atttgcgccg tcggcaccaa
    12241 tcaccagctt cgcgcggatc gtttctccat ccttcagttc cagctcctga agatcatcat
    12301 ggcgatgcag cgcaatcagt gagcctggca cacgtaacgt tactttcgga tgcgcttcca
    12361 gcgcctgcca cagcgcctgt tgcaggacgg tgttttccac catatagcca agcaacggta
    12421 gcttaagttc agcggcgtca aacaccacat gcgccgtttc ccactcccac gtttccagtc
    12481 tgcggtaagg atggcaacgc atagcctgta ctgcatccca gacccctaac cctttaagca
    12541 atgataccga agccgcgctg atcgccgaga tccgcacatc cggttggcta tcagcgacaa
    12601 acggtgcagg ttctgcgtgc tcgatcaccg ttaccgtaaa tccgtgctgt gccagcccca
    12661 gcgccagtgc gccgccgacc attcctacgc cgacaatggc aatttccgtt ggttgatttg
    12721 tcatggtcaa tcatcctgtt agcaatatgc tttaagttta caggattttt acccccaagg
    12781 gctgataacg ctcatactgg tcacaacggc agcaaagcat tacactatgc gccctgcatt
    12841 cctggctact atttcgcaag agcaagtcga tgaccaaaaa actccatatt aaaacctggg
    12901 gctgtcagat gaacgagtac gattcatcga agatggccga tctgctggat gccacccacg
    12961 gctatcaact gaccgacgtg gcggaagaag cggatgtgct gctgctgaac acctgctcaa
    13021 tccgcgagaa ggctcaggaa aaagtcttcc atcagttggg tcgctggaaa ctgttaaaag
    13081 agaagaatcc agacctgatt atcggcgtcg gtggctgcgt ggcatcgcaa gaaggcgagc
    13141 acattcgcca gcgcgcccac tatgtcgata ttatttttgg gccgcaaacg ctgcatcgtc
    13201 tgccggagat gatcaactcc gtgcgcggcg accgcagccc ggttgtagat atcagcttcc
    13261 cggaaatcga gaagtttgac cgtctgccgg aaccgcgcgc cgaagggccg accgcgtttg
    13321 tctccatcat ggaaggctgc aataaatatt gcacctactg cgtggtgcct tacacccgtg
    13381 gtgaagaggt aagccgtccg tccgacgata ttctgtttga aattgcccag cttgcggctc
    13441 agggcgtgcg tgaagtcaac ctgctcggtc agaacgtgaa cgcctggcgt ggtgaaaact
    13501 acgacggcac cactggatcg tttgccgatc tgctgcgtct ggttgcggca atcgacggga
    13561 tcgatcgtat tcgctttacc accagccatc cgatcgaatt caccgacgat atcatcgaag
    13621 tgtatcgcga cacgccggag ctggtgagct tcctgcatct gccggtacag agcggttccg
    13681 atcgcattct gaacctgatg ggccgtaccc atacggcgct ggagtacaaa gcgatcatcc
    13741 gtaaactgcg tgcggcgcgt ccggatattc agatcagttc tgacttcatc gttggcttcc
    13801 ctggagaaac caccgaagac ttcgagaaaa cgatgaagct gattgccgac gtcaatttcg
    13861 acatgagcta cagctttatc ttctctgcac gtccaggaac gcctgccgct gatatggttg
    13921 atgatgttcc ggaagaagag aagaagcagc gtctgtatat tctgcaagaa cgtatcaatc
    13981 agcaagcgat ggcatggagc cgtcggatgc tcggcaccac ccagcgtatt ctggtagaag
    14041 gtacatcgcg taagagcatc atggagcttt ccgggcgtac cgaaaataac cgtgtggtca
    14101 acttcgaggg cacaccggat atgatcggta aattcgtcga tgtagaaatt accgacgtct
    14161 acccgaactc tctgcgcggt aaagtggtgc gtactgaaga tgaaatgggt ctgcgcgtgg
    14221 cagaaacacc cgaatcggtg attgcccgta ctcgcaaaga aaatgacctt ggcgtgggtt
    14281 attatcagcc gtaattctca ggcccgccgt tccggtgggc cttgtttttt ctctctttat
    14341 ccccatcttt atcgcaatgc ttgcgccttt ttcctgtggc gtgaataatt tccttatagg
    14401 ccggtgaatg ttcagcttcc ggcggcatac agataagagg aacggtttga acatagacac
    14461 tcgcgaaatc accctggagc cagcagacaa tgcgcgtctg ttgagcctgt gcggcccgtt
    14521 tgatgacaac atcaagcagc tcgaacgtcg tctcggcatc gagatcaatc gccgcgataa
    14581 ccactttaaa ctgaccggcc gtccgatttg cgtcaccgct gcggcagaca ttctgcgtag
    14641 cctgtatgtc gatactgccc cgatgcgcgg tcagattcag gatatcgaac cggaacagat
    14701 ccaccttgcg attaaagaag cgcgggtact ggagcaaagc gcggagagcg tgccggagta
    14761 cggcaaagcg gtcaatatca aaactaaacg cggcgtaatt aagccgcgca cgccaaacca
    14821 ggcgcagtac atcgccaata ttctcgacca tgacattacc ttcggcgttg gcccggcggg
    14881 tacgggtaaa acctacctgg cagtggctgc ggcagttgat gccctggagc gtcaggaaat
    14941 tcgccgtatt ctgctgactc gtccggcagt agaagccggt gagaaactgg gcttcctgcc
    15001 tggcgattta agccagaaag tagacccgta tctgcgccca ctgtacgacg cgctgtttga
    15061 aatgctgggc tttgagaaag tcgagaaact gattgagcgc aacgttattg aagtcgcgcc
    15121 tctggcctat atgcgtggtc gtacgctgaa tgacgccttt atcattctcg atgagagcca
    15181 gaacaccacc atcgaacaga tgaagatgtt cctgacccgt atcggtttta actcaaaagc
    15241 ggttatcacc ggcgacgtca cacagatcga cctgccgcgt aatactaaat caggtttgcg
    15301 tcatgccatc gaagtgctgg ccgatgtcga agagatcagc tttaacttct tccacagcga
    15361 agacgtggtt cgtcacccgg tggtggcgcg tatcgttaac gcttatgaag cctgggaaga
    15421 agcagaacaa aaacgaaaag cggcgctggc ggcagaacgc aagcgcgaag aacaggaaca
    15481 aaaatgagtc aggtgatcct cgatttacaa ctggcatgtg aagataattc cgggttaccg
    15541 gaagagagcc agtttcagac atggctgaat gcggtgatcc cgcactttca gaaagaatcg
    15601 gaagtgacta ttcgcgtggt cgataccgcc gaaagccaca gtctgaatct gacctatcgc
    15661 ggtaaggata agccgaccaa cgtgctctcc ttcccgtttg aagtgccgcc tggcatggag
    15721 atgtcgctac tgggcgatct ggttatctgc cgtcaggtgg ttgagaagga agctcaggag
    15781 caaggcaaac cactggaggc gcactgggcg catatggtgg tgcacggcag tctgcatttg
    15841 ttaggttacg atcacatcga agatgacgaa gcagaagaaa tggaagccct cgaaacagag
    15901 attatgcttg ctctgggcta tgaggatccg tacattgccg agaaagaata atttgccagg
    15961 catttgactg gcagacgtcc ccgccgttgg tgattaacga cggcgttttg attaactaac
    16021 atgagaaccc atacgacgcc atgagcgacg acaattcaca cagtagtgac acgataagca
    16081 acaagaaggg atttttctcc ctgttactca gccaactttt ccacggtgaa ccgaaaaacc
    16141 gtgacgaact gctggcgctg atccgtgatt ccgggcagaa cgaccttatc gacgaagata
    16201 cgcgcgatat gctcgaaggg gtgatggaca tcgcagacca acgcgttcgc gacatcatga
    16261 tcccccgctc ccagatgatt accctgaaac gcaaccagac gctggacgaa tgccttgatg
    16321 tcatcatcga gtccgcccac tcacgtttcc cggtgattag cgaagacaaa gatcacattg
    16381 aagggattct gatggcgaaa gacttgctgc cgtttatgcg cagcgatgct gaagccttca
    16441 gcatggacaa agtgttacgt caggcggttg tcgttcctga aagtaagcgc gtagaccgga
    16501 tgctgaaaga gtttcgctct cagcgttacc acatggcgat cgtcattgac gaattcggtg
    16561 gggtttccgg tctggtgacc attgaagaca tcctggaact aattgttggt gagattgaag
    16621 acgagtatga cgaagaagat gatatcgact tccgtcagct gagtcgtcat acctggaccg
    16681 tgcgcgcact ggcttccatt gaagacttca acgaagcgtt cggcacccac tttagcgatg
    16741 aagaggttga cactatcggt ggtctggtga tgcaggcatt tgggcatctt ccggcgcgtg
    16801 gcgaaactat cgacatcgac ggttaccagt tcaaagtggc gatggccgac agtcggcgta
    16861 ttattcaggt tcatgtcaaa atcccggatg actcacccca gccaaagctg gatgaataaa
    16921 accgaaactg gatagataac tacatggctt ttgcctcatt aattgaacgc cagcgcattc
    16981 gcctgctgct ggcgttatta ttcggtgcct gcggaacgct ggccttctct ccttacgacg
    17041 tctggcctgc ggcgattatt tcgctgatgg ggcttcaggc gttgaccttt aaccgtcgcc
    17101 cactccagtc tgccgctatt ggcttttgct ggggatttgg cctctttggc agcggtatta
    17161 actgggtcta tgtcagcatc gcgacctttg gcgggatgcc tggcccggtt aacatcttcc
    17221 tggtggtgct gctggcggcg tatttgtcgc tgtataccgg actgtttgct ggcgtgctgt
    17281 cgcgtctgtg gccgaaaacc acctggctgc gcgtggcgat tgccgcccct tctctctggc
    17341 aagtgaccga gtttctgcgc ggttgggtac tgacaggctt cccgtggtta cagttcggct
    17401 atagccagat tgatggcccg ttaaaagggc tggcaccgat aatgggcgtg gaagccatta
    17461 acttcctgct gatgatggtt agtggcctgc tggcactggc gttggtcaaa cgcaactggc
    17521 gtccgctggt ggtggccgtc gtgctgtttg ccctaccctt cccgctgcgt tacatccaat
    17581 ggtttacccc acaaccggag aaaaccattc aggtttcgat ggttcagggc gatattccgc
    17641 aatcactgaa atgggatgga gatcagcttc ttaatacgct gaagatttac tacaacgcaa
    17701 cggctccgct gatgggcaaa tcatcgttga ttatctggcc ggagtcggcg ataaccgatc
    17761 tggaaattaa tcagcaacca ttcctcaaag cactggacgg tgagttgcgc gataaaggta
    17821 gctcgctcgt gaccgggatt gtcgacgcgc gtctcaataa gcagaaccgc tacgatacct
    17881 acaacaccat catcacactg ggtaaaggtg caccgtacag ctacgaatca gccgatcgct
    17941 ataacaaaaa ccatctggtg ccgtttggcg agtttgtccc gctggagtcg attctgcgtc
    18001 cgttagcacc gttctttgat ctgccgatgt cgtcgttcag ccgtgggcca tatatccagc
    18061 cgccgctgtc ggtaaatggt attgagctta ctgcggccat ttgctacgag atcattcttg
    18121 gcgagcaagt gcgcgataac ttccgcccgg ataccgacta tctgctgact atctccaacg
    18181 atgcgtggtt tggtaaatct attggtccat ggcaacactt ccagatggcg cgaatgcgtg
    18241 cgctggagct ggcgcgccca ctgttgcgca gcaccaacaa cggcattacg gcggtgattg
    18301 gcccgcaggg tgagattcag gcgatgatcc cgcagttcac ccgcgaggtg ttgaccacta
    18361 acgtgacgcc gaccaccgga ctcacaccgt atgcacgaac cggcaactgg ccgctgtggg
    18421 tgctgacggc actgtttggt tttgccgctg tgctgatgag tctgcgagca cgtaaacgtt
    18481 gatgtgcctg atgtatcctg catgagcaaa acgtactttc tcattgagct ttggggcatg
    18541 gttatcatgc ccctttttct tacggttcag ggatacaagc cacattgcca tattggggat
    18601 gacgaataat tgcctgcgga tcaagcgagc gcgtcctgtc cagttggatc aacccgttct
    18661 tcgccataac aaatattttt ctaccatccg ctgaaaaacg aaggtgatta acgtagctgg
    18721 caggataaac gccaataatc tgcccggcat taatattgag caggatgaca taaccttgca
    18781 ggccatatga caccaacgct aaatactgac cgtccgggct aataccctca acggaataaa
    18841 cgctcctgct acaccattgc gtcataatgc tggcttgttg atcccctacg cttatacgtg
    18901 aaatcccctg gctgtcggca aaaattacat ccccgttttc aggtcgaaaa cgcatcgccg
    18961 atatcgttcc tcgcgcatca ctagccggat agctttcttt ctgatgcgtg atggtattga
    19021 tgcgtacaat gtcacctctg accataccgg gtatcaacaa tattttttca tccggggaaa
    19081 gagccataac ccaggcacca ggtttattac tcgtccccgt cccgtcgtat ttctccagcg
    19141 ggatatccgc cacatgctga taagtcacca gatcaatcgc tgacacacta ttactggtgc
    19201 gattcatcac ccaggctttt gtgccatcac gcgagaggat aaggctgtca ggatgtgcac
    19261 cggtggtaat cgactgaata atctcccctg tcgccacgtt aatgaccgat aacgcattcg
    19321 cttcaaatga agtcgcataa atcaactttt catccgggct taatgccagc gatctgacgc
    19381 cctgatcttt acagccacca caatgtttga ctgacttttc tttcgctatg ggcgctgtaa
    19441 aggtatgcag gattttcttt tgtccgaggt cgaaaacaac aatgctgttc gtgttttcaa
    19501 ttgcggcata caacatatta ccatcccgac tgatcgccat atcatggata tcggcaatct
    19561 cagcgagcaa atcaccactg gcctgttcac gacttaccgg agcggataat gtttccctga
    19621 gggcgttgcg ttcctccgct aactgctgct ttttttgccg tcgctcttgt tcaatctcct
    19681 gtggataaaa cgcttcacgc aggctttgcc tgacttgtaa aactgactca tccatctgct
    19741 gctgcgcaag ccagatagcc ccggtcagaa gaagactgcc cgcaacaata atcccgcctt
    19801 ccgtaacgag tcggttttgc ccagataaac gcgcgctcag ccaacccaac gcgccacccg
    19861 caatcaggcc gccaatgtga caagcattat ctattcctgt ttgccgggca ccatacaaca
    19921 gtgtcagcgc caccataccc aacagagaaa atactcgcct ctggttttta tggtatgtgc
    19981 ccgcacctga agcaaactgt gttgctatcg ctgcaccagc tattcccaga atcgccccgg
    20041 atgccccaac gctgatgact atctgctcac tttcgcgtaa cgcataacag gcgctgatga
    20101 gcccaccaaa gacgccggag aataaccagg taatcaggag tcgccatttc cccagaatct
    20161 gttccgccac gcctcccacg accaggaatg caaacatatt catcagcaga tgagaaacac
    20221 tactgtgcag aaagatactg ctcaataagc gccagggttc acccgacaaa gtaagaggcg
    20281 caacgtttgc gccgagatgg ataagtgcgg attgatcgac ctcttcataa ctcatccatt
    20341 gtgcgagata acaaaatatc ccaatacaga gtgcaaaaag gataagggtt attgcgggta
    20401 attgaacatt taacggctta accgaagatg cagacataag gtgtttcttc cttgaaaaga
    20461 caggtaaaaa gcatttttcc cgtgtgcagt tatcatcccg gcaaacgtcc catgccgaaa
    20521 agataattct gtccgaaata tgactttttc agcctaaaat tacaatcgta attaattcgc
    20581 ccctaagtat tttttggcat gtaagttgct ttatttactg tgtaagcgat gaaatcagcc
    20641 gcgacgcaac caggttgcac caccacaaat cagcggtagc accaaaaagg tgcggtttaa
    20701 gaattttgct tcggaatggt gcggcgcatc tcgcaaaaac aaacaataaa acagcaatat
    20761 ctttacattt agccaaacta aatgttacct aacaggcaca acactgcaca ataaagttgc
    20821 agacgataac aacacaaaca ctcacaacgg gtatccatgc gttcttaacg cagaagataa
    20881 aggagttgga tatgcaatta cgtaaacctg ccacagcaat cctcgccctg gcgctttccg
    20941 caggactggc acaggcagat gacgccgccc cggcagcagg cagtacgctg gacaaaatcg
    21001 ccaaaaacgg tgtgattgtc gtcggtcacc gtgaatcttc agtacctttc tcttattacg
    21061 acaatcagca aaaagtggtg ggttactcgc aggattactc caacgccatt gttgaagcag
    21121 tgaaaaagaa actcaacaaa ccggacttgc aggtgaaact gattccgatt acctcacaaa
    21181 accgtattcc actgctgcaa aacggcactt tcgattttga atgtggttct accactaaca
    21241 acgtcgaacg ccaaaaacag gcggctttct ctgacactat tttcgtggtc ggtacgcgcc
    21301 tgctgaccaa aaagggtggc gatatcaaag attttgccga cctgaaagac aaagccgtag
    21361 tcgtcacttc cggcactacc tctgaagttt tgctcaacaa actgaatgaa gagcaaaaaa
    21421 tgaatatgcg catcatcagc gccaaagatc acggtgactc tttccgcacc ctggaaagcg
    21481 gtcgtgccgt tgcctttatg atggatgacg ctctgctggc cggtgaacgt gcgaaagcga
    21541 agaaaccaga caactgggat atcgtcggca agccgcagtc tcaggaagcc tacggttgca
    21601 tgttgcgtaa agacgatccg cagttcaaaa agctgatgga tgacactatc gctcaggtgc
    21661 agacctccgg tgaagcggaa aaatggtttg ataaatggtt caaaaatcca attccgccga
    21721 aaaacctgaa catgaatttc gaactgtcag acgaaatgaa agcactgttc aaagaaccga
    21781 atgacaaggc actgaactaa ttacaagaac caggggcgga aattccagcc ctctcgattg
    21841 ttacgtagca cggacagact atacgcctga tggtcgttcc ccatcgggcc tgaaaaccgc
    21901 aatacgctgg gtaacaatct tcgagggtag cagttaacgc tgctaccctc ttttttctgg
    21961 agtagattta tgtctataga ctggaactgg ggtatttttt tacaacaagc cccgttcggc
    22021 aacaccacct atcttggttg gatctggagt ggttttcagg tgacgatcgc tttgtcgatc
    22081 tgcgcctgga ttatcgcttt cctcgtcggt tcatttttcg gcattttacg taccgttcca
    22141 aaccgttttc tctccggtct gggtacgttg tatgtcgaac tgttccgcaa cgtgccgttg
    22201 attgtgcaat tctttacctg gtatctggtg atcccggagc tgctgccgga gaaaatcggc
    22261 atgtggttta aggccgagct ggatcccaat attcagtttt tcctttcatc catgctctgc
    22321 ctggggctgt ttaccgccgc ccgtgtttgc gaacaggtac gcgccgctat tcagtcgctg
    22381 ccgcgtgggc aaaagaatgc cgcgctggcg atgggcctga cgctaccgca ggcttatcga
    22441 tacgtactgc tgcctaatgc ttatcgcgtt atcgtcccgc cgatgacctc agagatgatg
    22501 aacctggtga aaaactccgc catcgcctcg actattggtc tggtggatat ggcggcgcag
    22561 gcgggtaaat tgcttgatta ctcagcccac gcctgggagt cgtttaccgc catcacgctg
    22621 gcgtatgtat tgattaacgc ttttatcatg ctggtgatga cgctggttga acgtaaagtt
    22681 cgcctgcctg gcaacatggg gggtaaataa tgtacgagtt tgactggagt tccattgtcc
    22741 cttccctgcc atatctgctc gacgggctgg tgatcaccct gaaaatcacc gtcacggcag
    22801 tcgtgattgg tattttgtgg ggcaccatgc tggcggtgat gcgcttgtcc agctttgcgc
    22861 ctgtagcgtg gtttgccaaa gcctacgtta acgtattccg ctcgattcct ttagttatgg
    22921 ttttgctgtg gttttacctg attgtgccgg gttttctgca aaacgtgctg ggattatcac
    22981 caaaaaatga tatccgttta atttcggcga tggtggcgtt ttcgatgttt gaagcggcct
    23041 actattcaga gattatccgt gctggtattc aaagtatttc tcgtggtcag tcgagcgccg
    23101 cgctggcgtt gggaatgact cactggcagt cgatgaaact gattattctg ccgcaggcgt
    23161 tccgcgcgat ggtgccgctg ctgctcactc agggcatcgt actgttccag gatacctcgc
    23221 tggtgtatgt gttatctctg gccgatttct tccgtaccgc ctcaaccatt ggtgagcgtg
    23281 atggtacgca ggttgagatg atcctgtttg ccggatttgt ttatttcgtt attagtctta
    23341 gcgcgtcgtt gttggtcagc tacttgaaaa gaaggacagc ataatgatta ccctgaaaaa
    23401 tgtttcaaaa tggtatggtc actttcaggt gctgaccgac tgctcaaccg aagtgaaaaa
    23461 aggcgaagtg gtggtggttt gcggcccgtc tggttccggc aaatcaacgc tgattaaaac
    23521 cgtcaacggc ctcgaaccag tgcagcaagg tgaaatcacc gtcgatggta tcgtggttaa
    23581 tgacaagaaa accgatctgg caaagctgcg ttcccgcgtc gggatggtat tccagcattt
    23641 cgagctgttc cctcatctgt cgattatcga aaacctgacc ctggcgcagg tgaaagtgct
    23701 taaacgcgat aaagcgccgg ctcgtgaaaa agcattgaaa ttgctggagc gcgtgggact
    23761 ttccgcacat gccaataaat ttccggcgca gctttccggc ggtcagcagc agcgtgtggc
    23821 gatcgctcgc gcattgtgta tggatcctat tgcgatgctg ttcgacgaac cgacgtcggc
    23881 gctggatccg gagatgatca acgaagtgct ggacgtgatg gtggaactgg cgaacgaagg
    23941 aatgaccatg atggtggtga cccacgaaat gggctttgcc cgcaaagtgg cgaatcgggt
    24001 gatctttatg gatgagggta aaattgtcga agactcgccg aaagacgctt tcttcgatga
    24061 tccgaaatcg gaccgcgcaa aagacttcct cgcgaaaatc ctgcattaat cactctggcg
    24121 cgcataccca agtgcgcgcc atctcacgct ttatcgtttc gcccctcgta tctgttacat
    24181 cctggcgtta cccttgtcgc aaagaagcac acaacaagga gcaacaatgg cactgccaat
    24241 tctgttagat tgcgacccag gtcatgacga cgctatcgca atagttctcg ccctcgcctc
    24301 accagagctt gatgtcaaag caattacgtc ttccgccgga aaccagacac cagaaaaaac
    24361 cttacgcaat gttcttcgta tgctgacctt gctcaatcgc accgatattc cggtagcagg
    24421 cggcgcggta aaaccgttaa tgcgtgagtt gattatcgcg gacaatgtgc acggcgaaag
    24481 cggtctcgac ggcccggcat taccggaacc ggcgttcgca ccgcaaaact gtacggcggt
    24541 agagctaatg gcgaaaacgc tgcgtgaaag tgcggaacct gtcacgattg tgtctaccgg
    24601 accgcaaact aacgttgcct tgctgctcaa tagccatccg gaactgcata gcaaaattgc
    24661 ccgtatcgtg attatgggcg gcgcaatggg gcttggtaac tggacgcctg cggctgaatt
    24721 taatatttac gttgacccgg aagcggcaga aattgtcttc cagtcaggga tcccggtggt
    24781 gatggctggt ctggatgtta ctcacaaagc acaaatccac gttgaagaca ctgagcgttt
    24841 ccgcgcgatt ggcaaccctg tttcaaccat tgttgccgaa ctgctggatt tcttcctcga
    24901 atatcataaa gacgaaaaat ggggctttgt cggcgcaccg ctgcatgacc catgcaccat
    24961 cgcctggctg ttgaaaccgg agttgtttac cactgttgag cgctgggttg gcgtggaaac
    25021 acaggggaaa tatactcagg gtatgacggt tgttgattat tattatctga ctggcaataa
    25081 accgaatgcc accgtaatgg tcgatgttga tcgtcagggc tttgttgatt tactggctga
    25141 tcgtctgaaa ttttacgctt aacacacgtc attgattaag atcaccacgc gttacctggg
    25201 tggtcttaat ctgtttatct ctttttcttt caagatataa atgcaatgga taatgcagaa
    25261 ctcgccattg gtatcgatct cggtactacc aatagtttaa ttgccgtctg gaaagacggt
    25321 gccgcgcaat taattccaaa taagttcggt gaatatttaa caccatccat aattagcatg
    25381 gatgaaaata atcatatttt agtcggaaaa ccggctgtat cacggcgtac ttcgcatccg
    25441 gataaaacgg cagcgttatt taaacgtgca atgggcagta ataccaactg gcggttaggc
    25501 agcgacacat ttaacgcgcc agaactgtcc tctttggtat tacgctcatt aaaagaagat
    25561 gccgaagaat ttctgcaacg tccgattaaa gatgtggtga tctccgttcc ggcttatttc
    25621 agcgatgaac aacgcaagca tacccgttta gcagcggagt tagccgggtt aaatgcggta
    25681 cgcttaatta atgaacccac agcagctgcg atggcgtatg gcctgcatac ccaacaaaat
    25741 acccgttcgc tggtgtttga tctcggtggc ggcacgtttg acgttacggt gcttgagtac
    25801 gccacgccgg tgattgaagt tcacgcctcc gctggcgaca acttcctcgg tggcgaagat
    25861 tttacccata tgttggtcga tgacgtttta aagcgcgcgg atgtcaccag gaccacgctt
    25921 aacgagagtg aactggcagc cttgtatgcc tgtgtggaag cggcaaaatg tagcaatcaa
    25981 tcgccattac acattcgctg gcagtaccag gatgaagcac gggaatgcga attttatgag
    26041 aatgaactgg aagatttgtg gttgccgctg ctcaatcgct tgcgagtgcc gattgaacag
    26101 gcgttgcgcg atgcgcgtct gaagccaagt caaatcgaca gtctggtact ggttggcggc
    26161 gcgtcacaaa tgccgctggt gcagcgaatc gccgtgcgtc tgtttggcaa attaccgtat
    26221 caaagttacg acccgagcac cattgtcgcg ctgggcgcag caatccaggc cgcctgccgc
    26281 ttacgcagtg aagatattga agaggtgatc ctcactgata tttgccctta ctcgttgggc
    26341 gttgaagtta accgctaggg catttccggc attttctcgc caattattga acgaaacacc
    26401 acagtaccgg tgtcgcgtgt agaaacttac tcgaccatgc acccggaaca ggattctatt
    26461 acggttaacg tctatcaggg agaaaaccac aaagttaaaa ataacattct ggttgaatcc
    26521 ttcgatgtgc cgttgaagaa aaccggggct tatcagtcga ttgatattcg ctttagttat
    26581 gatatcaacg gcttgcttga agttgacgtg cttctggaag atggcagcgt taagtccaga
    26641 gtcattaatc atagcccggt aacattgagc gcgcagcaga ttgaagagag tcggacgcgg
    26701 ttatccgcat tgaaaattta tccgcgcgat atgctcatca accgcacctt taaggccaaa
    26761 ctggaagagt tgtgggcgcg ggcgctgggt gacgagcgag aagagattgg ccgggtgatc
    26821 accgattttg atgcggcgtt gcagtcaaac gatatggccc gcgtcgatga ggtcaggcgg
    26881 cgggcgagcg tttatttagc cattgagacc tcataatcac ggctgccgga tgcggcgtaa
    26941 atgctgcatc cggcatcggc gcaaaaaaca catcgtcaac taaacaacat cgcgattttg
    27001 ccgagagcat aacctataac cagcaccgtt aaactgattt tgataatgct ggtaatgctc
    27061 tgtggttttt caccctcatc atttttttct tcatggtttt caggtgttgg ctcatcgaca
    27121 tcttcatcct ccggctcgag ttttactttc gtcaacagca tccgggtgat aatggcctct
    27181 aatggatgag catctccgcc ttccagcctt gtattaagac gggccagcat ctcttgtttc
    27241 ggaaacgctt caagtactac ctgttccgct acccgataca aatccagtaa cggtgaatct
    27301 tcttctgtct cgctaccgac tctcgatttc caccacgccc atgctttgcg tttgaagaat
    27361 aacgactcaa taaacgattg acctaacggc gataattttc cgctatgtaa aatttccgcc
    27421 cataacagcc gcgtccgggc gctctcacca ggaacatatt tcagcggttg accttgcgca
    27481 tctaccatgc tccgcgcttc gataaccatc actaataacg cgaaataatc cgggcaatga
    27541 cgcttgctcc actgcaacaa caaattatca gcttgtgtag atgggtaact ttcccggtac
    27601 gcgcagagat cggcaagcag gctttccccc tcgccgcagt acaacctttg agcacacaag
    27661 taatatcggg catcttcgct ttctggttct tccatttgcc actgtttggc tacaggaatg
    27721 agttccgcca tacctgtttg caccgagctg taccagcgca acagtttgcg atgtaacttt
    27781 gcatcatcag gaatgaacca cggaccttcc atcgccaaaa acgcagtgac gtagtcagga
    27841 tggtaacggc agcaacgttc cagcgcgaag taatactcaa cagtctggtt ctgcaacgca
    27901 acgggatatt ccagaaggct aaggaaatcg aaacaatcac cagcctgaat ggcctcaaga
    27961 aaagcctccc tttcttcctc atccattcct tcgctgtcat taaacgaatg ccagttcaaa
    28021 cgctctgcca gcaaagaagc acaatttaat gaaagatatc gcgcttctat agcgattgca
    28081 cacagcggcc agcgtaattg atcgacatcc tccatgttcc aggtatttaa ttgctgaata
    28141 aatttctgcc aggcggaggg ctgaaaacga tcactttccg aatccagtaa tgtcctgaat
    28201 gcacgcagta tttcatgttc agcggcggca tctttttctt catcatctgc ttcctcgaca
    28261 ggatttaccg ccagtcgcag cgcctcttca taggcctggc gtaatgcttt aaacccctgc
    28321 ggatcggttt cgggatgaca taacggtaag cgagcaagat aagcctggcg gataatgtct
    28381 atttgcgtcg tgctttcaat ttcgaggatt tgccaacatg ttttcatagg tcgctcacat
    28441 ccttaagcgt taccgggcat tcaggctgga cagttaatgg ctgccaggga agcgtggcat
    28501 agaaacgatc gcttcccgct gccagctctt cgaacatccg actcccggcg ctatcgtatt
    28561 tgtacagggt tagcgcctcg cgtagcgaag tgttgtcttc gcactgagaa gattgccagt
    28621 agagacgtcc taacgaatag gcggaaaaat agtgcatcca gctgtgatag taatggtgag
    28681 ctcgtacata gacgcgcgat tgcagccata aactctcttc ttccgtcagc cagttgttga
    28741 gcacaccgac ccgtgacaga aaccccattc gaacataatc ccaggctttg attccccctt
    28801 ctccacagca catagcggtt tgggcaacga attgggtata aatcaaccct ctctccgagc
    28861 caccagcaat taatgctttc cactcttcag gggaagagcg atgccatgtc agatataagc
    28921 cagctaaatc attggcatga ccatcatcag tcatctgata aatagtctga attaaatcat
    28981 cacgattatt tattccccag gatgatgaga gatcgaattc ttttatcccg gtatacattt
    29041 ttggcgagtc atgcgcgggt ttgcaaccct gattaagtat ttccatgggc gcagaaagcg
    29101 caaaaagaag atattgttct tccttattca tttgttaata atcccttaag tgcattaact
    29161 gactactttt ttattattga tgagtataaa cttgacgctt tcaaaataaa aagaaaatcg
    29221 aagcattcac acatgaataa aaaattaatg tatatattcg caatttttat agttgcagca
    29281 attacctgta ttagccaacc caagaaaacg acgttgcgtg ataaagccat ggtgaattat
    29341 gcctttgatt atttaagatc accgggcagt cttccattca ccacggcagc cacggagctt
    29401 tccgcgattc atggtcactc aacgtcgcaa tatcgccttg gagaatttta tcttcatggt
    29461 agcgacggta aaccactgga ttatacacag gcgagatact ggtatgagca atcagcggaa
    29521 caggaaaatc cacgcgcgca aagtaaactg gggtggatct acctcaaagg tctgggggtc
    29581 aaacccgaca cccgtaaagc aattctctgg tataaggaag cagctgaaca agggtatgct
    29641 catgctcaat atactttagg tttgatctac agaaatggct caggtattaa tgttaaccat
    29701 tatgaatctc aaaaatggtt aaaactggcc gccaaacaac attacaaaaa tgcggaaaga
    29761 ttacttgccg ggcttcccgc acattaacgt cgactgtcac ccgaacagat ggagtatttt
    29821 ccctatcaaa ccagcaaaga taaaaatata gaaaataatt ttaatgatgc ccagacagcc
    29881 tggcccacca tcgtcaacgg gcattggctg cggttgcggc tccggttcaa cagtttgctt
    29941 ctcggtaaaa ttcgccgcct gaattaataa tcgacgaata attaatgctt ccagtggaca
    30001 acgattttct gcatcaaggc ggctcacaat cggcattaat gattcttctg taggttggca
    30061 ggcctgcact aattgtttgg caactagata taatggccat aagggatgtt ctgtatcacg
    30121 cgtgagtgag atccgcggct tacgtccttt gtgcaaaacc atctcgacca gtgaacgccc
    30181 cagcgcagag agtttttcat cgctgagtat ttcgtataaa tgaaaacgcg tacgggcgct
    30241 gtcgccgggg acataaagta gcggttttcc ctgatcgtta accagatcac gcgcttcaat
    30301 catcatcacc agtaatgggt aataatcgac ccgatgctga cggcaccatt gcaacattaa
    30361 agcatccgcc tgggtggagg gatactcgcg ccagtagtca caaagttctg ccaacaggct
    30421 ttcgccttct ccacaataga cgcgttgcgc gtattcataa taaggcgcat cttcattatc
    30481 aggttcagct tcccgccatg actgggcgta atccagaagt tcggggattc ccaagcggct
    30541 tgcgctatac cagcggagta aatttctgtg cagttttgca tcatcgggga tgatcactgc
    30601 gcgatgttgc gctaaataca atgtcagcca ttctggatgc gatgaccaaa tccgagcgaa
    30661 catttggtaa aaatcgatgg tgtcattttg cacggcaacg ggcagatgca gaatggtctg
    30721 gaagttaaaa acattcccct tcgcaatggc atataaaaag gattccagtt cttcttcgtc
    30781 tatttccccg acgttttcct cctgcaacca ccgcaatctt tctgctaata acaccacgca
    30841 ctcgaaggat aaatgggcag tgttcatggc gattgtgcac agcgaccagc gtaattcatc
    30901 aatctcttcc atcgagcaat aatttaattg ctgaatgaat cgctgccagg cggagggcag
    30961 aaaacgttcg ctatcagagg caagtaacgc gcgaaaggcg agcagaattt catgttctgc
    31021 tacctcatat tcttctggtt gccaaacaga tttagcaggc gactgcgcaa tccggagcgc
    31081 ctcctcatac gcctgacgaa gttgtttaaa accctgcgga tcggtttctg gatgaaagga
    31141 cggtaacagc gccagataag cgcggcggat aatatcgacg tcagtcgttt cctctatatc
    31201 gaggatcttc cagcaatttt tcataggtcg ctcatatcct taagcgtttc cgggcatgca
    31261 gaatagtaag ccaatggttg ccaggacaag gtagggtaat acgcatcatc actggcaaat
    31321 aattgataaa acatatcatt gccggaatcg tcgtattctt tacgcgctaa gaattcgcgt
    31381 aataaaggca gatgatcatc ttcgggagac tgccaatatt gccgaccaaa ggtataaccg
    31441 gcaaagtatt gccgccagtt actataataa cgtagagccc gtagatgaat gcgcgactgg
    31501 atccatagac tttcttcttc gctcaaccag ttattaagta cgcccattcg gcttaaaaaa
    31561 cccatccgga cataatccca ggctttgatt cccccttcgc cacagcattc ggcggtgctg
    31621 gcgacaaatt gcgcataagc ctgaccttgc tcatttagtt cagcaagata gtcacgccat
    31681 tcacatggcg aataacgaaa ccagcggtga taaaaccctg ccaaccgagc ggcatgaccg
    31741 ttatcgatca ttcggtgaat tgtgcgcagc aggtcatcac ggtcatcaat tccccatgag
    31801 gaagaaaggt caaaaaagca tgtacctaaa tacattttgg gtgatgaatg cgaaggtaaa
    31861 cagcattcat tgcgtatttc catcggagtg gaaagtgcaa acaatatttt ttgcgactcc
    31921 atgtccatat aatttatcct taaatattca ttaaatgaat attagccgca ttgcgaggct
    31981 cgtttattta tacttttctg acgcgacata atacgcataa aataaggctt acgcaaaaat
    32041 ggaacacata aaaatataat atgttttatc tgcaaccata aggaaatgat aatgattttc
    32101 acgtcaagtt gctgcgataa tttatcaata gatgagatta tcgaacgtgc tgaaaaaggc
    32161 gattgcgagg ctcaatatat tgttgggttt tattataatc gcgatagcgc aattgattct
    32221 ccggacgacg aaaaagcctt ttactggctg aagctggccg ccgagcaagg tcattgtgaa
    32281 gcacaatatt ccttagaaca aaagtatact gaggataaaa gccgtcataa agataatgag
    32341 catgcaatct tttggctgaa aaaagctgcc ctacaaggcc atactttcgc ttccaacgcc
    32401 cttggctgga tactggatcg tggagaagac cccaattata aagaagcggt tgtctggtat
    32461 cagatagccg cggagagcgg aatgtcttat gcgcaaaata atcttgggtg gatgtacaga
    32521 aatggcaacg gagtcgcaaa agactatgcg ctggcatttt tctggtacaa acaagctgca
    32581 ttacaaggcc atagtgacgc gcaaaacaat ctggccgatc tttatgaaga cggaaaaggc
    32641 gttgctcaaa acgagacact cgccgcattc tggtatttga aaagcgcaca gcagggtaat
    32701 cggcatgctc agtttcaaat tgcatgggat tataacgctg gcgaaggggt ggaccaggac
    32761 tataagcaag cgatgtactg gtatctgaag gctgccgctc aggggagcgt cggcgcttac
    32821 gtcaacatcg gttatatgta taaacacgga caaggcgttg agaaagatta tcaggctgcc
    32881 tttgaatggt ttacgaaagc cgctgaatgc aatgacgcca ctgcctggta taacctggcc
    32941 attatgtatc actacggaga aggaagacct gtcgatctcc gacaggctct cgacctgtat
    33001 cgtaaagttc agtcatccgg aaccagagat gtcagtcaag aaattcgtga gattgaagat
    33061 ttactgtaga gataaaaaca agggccgggg aatttcattc ggcccatatt ctctttattg
    33121 ctgtgcgaaa tcagactatt acggctcaaa cgggcgtctc tggaactggt catgaccaca
    33181 tttcgggcat agcgtcagca cttccggtgt gtagatcggg agatggaagt gacatttctc
    33241 gcagaccaga tttcccagcc cgaccacttc tccgctgtga taaaccccat gatgattgag
    33301 gtcctggaaa acttcgcgcc attcaagctg cgttttatcg gtgatgtctg ccagctcctg
    33361 ccacaagctt tctttaatca cccgcataaa gacgctgtca gattcttctt tcaggctctc
    33421 ttcatagctc atggcgaact cttccaggtc acgtctgaca gctcgcgtca gctcatcgac
    33481 ctcggttcgc gttaactccc ctgtttttat tacgcgctcg cgcgcctgtt ccaccagtgc
    33541 gtcgatatca cgttcgccat tgcgcaggcg ttcgctcagt gacgcgacca gttcacggta
    33601 atattgagca accttgttca tcgtttcatc tcccgggtcg ttaactgact ataataatag
    33661 acgttctttg ttccttgctt tgctaatacg cccacaaact ctgtaaatta atgtatgaat
    33721 aatccgggca tcatttcaac gtcgagtgcg cgaaaggctg ttttgacgcg gacgtttggg
    33781 ctatgctatg cggatctgaa aaaccacctc aacgctacat ttgtagccgt attgaaaaca
    33841 ggaccactgg ctgccatgca agagcaatac cgcccggaag agatagaatc caaagtacag
    33901 cttcattggg atgagaagcg cacatttgaa gtaaccgaag acgagagcaa agagaagtat
    33961 tactgcctgt ctatgcttcc ctatccttct ggtcgactac acatgggcca cgtacgtaac
    34021 tacaccatcg gtgacgtgat cgcccgctac cagcgtatgc tgggcaaaaa cgtcctgcag
    34081 ccgatcggct gggacgcgtt tggtctgcct gcggaaggcg cggcggtgaa aaacaacacc
    34141 gctccggcac cgtggacgta cgacaacatc gcgtatatga aaaaccagct caaaatgctg
    34201 ggctttggtt atgactggag ccgcgagctg gcaacctgta cgccggaata ctaccgttgg
    34261 gaacagaaat tcttcaccga gctgtataaa aaaggcctgg tatataagaa gacttctgcg
    34321 gtcaactggt gcccgaacga ccagaccgta ctggcgaacg aacaagttat cgacggctgc
    34381 tgctggcgct gcgataccaa agttgaacgt aaagagatcc cgcagtggtt tatcaaaatc
    34441 actgcttacg ctgacgagct gctcaacgat ctggataaac tggatcactg gccagacacc
    34501 gttaaaacca tgcagcgtaa ctggatcggt cgttccgaag gcgtggagat caccttcaac
    34561 gttaacgact atgacaacac gctgaccgtt tacactaccc gcccggacac ctttatgggt
    34621 tgtacctacc tggcggtagc tgcgggtcat ccgctggcgc agaaagcggc ggaaaataat
    34681 cctgaactgg cggcctttat tgacgaatgc cgtaacacca aagttgccga agctgaaatg
    34741 gcgacgatgg agaaaaaagg cgtcgatact ggctttaaag ctgttcaccc attaacgggc
    34801 gaagaaattc ccgtttgggc agcaaacttc gtattgatgg agtacggcac gggcgcagtt
    34861 atggcggttc cgggtcacga ccagcgcgac tacgagtttg cctctaaata cggcctgaac
    34921 atcaaaccag ttatcctggc tgctgacggc tctgagccag atctttctca gcaagccctg
    34981 actgaaaaag gcgtgctgtt caactctggc gaattcaacg gtcttgacca tgaagcggcc
    35041 ttcaacgcca tcgccgataa actgactgcg atgggcgttg gcgagcgtaa agtgaactac
    35101 cgcctgcgcg actggggtgt ttctcgtcag cgttactggg gcgcgccgat tccgatggtg
    35161 acgctggaag acggtaccgt aatgccgacc ccggacgacc agctgccggt gatcctgccg
    35221 gaagatgtgg tgatggacgg cattaccagc ccgattaaag cagatccgga gtgggcaaaa
    35281 actaccgtta acggtatgcc agcgctgcgt gaaaccgaca ctttcgacac ctttatggag
    35341 tcctcctggt actatgcgcg ctacacttgc ccagagtaca aagaaggtat gctggattcc
    35401 gaagcggcta actactggct gccggtggat atctacattg gtggtatcga acacgccatt
    35461 atgcacctgc tctacttccg cttcttccac aaactgatgc gtgatgcagg catggtgaac
    35521 tctgacgaac cagcgaaaca gttgctgtgt cagggtatgg tgctggcaga tgccttctac
    35581 tatgttggcg aaaacggcga acgtaactgg gtttccccgg ttgatgctat cgttgaacgt
    35641 gacgagaaag gccgtatcgt gaaagcgaaa gatgcggcag gccatgaact ggtttatacc
    35701 ggcatgagca aaatgtccaa gtcgaagaac aacggtatcg acccgcaggt gatggttgaa
    35761 cgttacggcg cggacaccgt tcgtctgttt atgatgtttg cttctccggc tgatatgact
    35821 ctcgaatggc aggaatccgg tgtggaaggg gctaaccgct tcctgaaacg tgtctggaaa
    35881 ctggtttacg agcacacagc aaaaggtgat gttgcggcac tgaacgttga tgcgctgact
    35941 gaagatcaga aagcgctgcg tcgcgatgtg cataaaacga tcgctaaagt gaccgatgat
    36001 atcggccgtc gtcagacctt caacaccgca attgcggcga ttatggagct gatgaacaaa
    36061 ctggcgaaag caccaaccga tggcgagcag gatcgcgctc tgatgcagga agcgctgctg
    36121 gccgttgtcc gtatgcttaa cccgttcacc ccgcacatct gcttcacgct gtggcaggaa
    36181 ctgaaaggcg aaggcgatat cgacaacgcg ccgtggccgg ttgctgacga aaaagcgatg
    36241 gtggaagact ccacgctggt cgtggtgcag gttaacggta aagtccgtgc caaaatcacc
    36301 gttccggtgg acgcaacgga agaacaggtt cgcgaacgtg ctggccagga acatctggta
    36361 gcaaaatatc ttgatggcgt taccgtgcgt aaagtgattt acgtaccagg taaactcctc
    36421 aatctggtcg ttggctaagc gcgggaggaa gcgtgcgata tctggcaaca ttgttgttat
    36481 ctctggcggt gttaatcacc gccgggtgtg gctggcatct gcgtgatacc acgcaggttc
    36541 cttccactat gaaggtcatg atcctggact caggcgatcc gaacgggcca ttaagccgtg
    36601 cggtgcgtaa ccagttacgt ctgaatggtg tcgagttgct tgataaagaa accacgcgta
    36661 aggacgttcc atccctgcgt ttgggtaaag tgagcatcgc gaaagatacc gcatcggtat
    36721 tccgtaacgg tcaaacagca gagtatcaga tgatcatgac ggtgaatgcg accgtgttga
    36781 tccccggccg tgatatctac ccgattagcg ccaaagtctt ccgttcgttc tttgataacc
    36841 cgcaaatggc gttagcgaaa gataacgaac aagacatgat cgtaaaagag atgtacgacc
    36901 gtgctgccga acagctgatt cgtaagctgc caagcatccg tgctgcggat attcgttccg
    36961 acgaagaaca gacgtcgacc acaacggata ctccggcaac gcctgcacgc gtctccacca
    37021 cgctgggtaa ctgatgattc ggttgtaccc ggaacaactc cgcgcgcagc tcaatgaagg
    37081 gctgcgcgcg gcgtatcttt tacttggtaa cgatcctctg ttattgcagg aaagccagga
    37141 cgctgttcgt caggtagctg cggcacaagg attcgaagaa caccacactt tttccattga
    37201 tcccaacact gactggaatg cgatcttttc gttatgccag gctatgagtc tgtttgccag
    37261 tcgacaaacg ctattgctgt tgttaccaga aaacggaccg aatgcggcga tcaatgaaca
    37321 acttctcaca ctcaccggac ttctgcatga cgacctgctg ttgatcgtcc gcggtaataa
    37381 attaagcaaa gcgcaagaaa atgccgcctg gtttactgcg cttgcgaatc gcagcgtgca
    37441 ggtgacctgt cagacaccgg agcaggctca gcttccccgc tgggttgctg cgcgcgcaaa
    37501 acagctcaac ttagaactgg atgacgctgc aaatcaggtg ctctgctact gttatgaagg
    37561 taacctgctg gcgctggctc aggcactgga gcgtttatcg ctgctctggc cagacggcaa
    37621 attgacatta ccgcgcgttg aacaggcggt gaatgatgcc gcgcatttca ccccttttca
    37681 ttgggttgat gctttgttga tgggaaaaag taagcgcgcg ttgcatattc ttcagcaact
    37741 gcgtctggaa ggcagcgagc cggttatttt gttgcgcaca ttacaacgtg aactgttgtt
    37801 actggttaac ctgaaacgcc agtctgccca tacgccactg cgtgcgttgt ttgataagca
    37861 tcgggtatgg cagaaccgcc ggggcatgat gggcgaggcg ttaaatcgct taagtcagcc
    37921 gcagttacgt caggccgtgc aactcctgac acgaacggaa ctcaccctca aacaagatta
    37981 cggtcagtca gtgtgggcag agctggaagg gttatctctt ctgttgtgcc ataaacccct
    38041 ggcggacgta tttatcgacg gttgatatga aatctttaca ggctctgttt ggcggcacct
    38101 ttgatccggt gcactatggt catcttaaac ccgtggaaac gctggcgaat ttgattggtc
    38161 tgacgcgggt cacaatcatc cctaataatg ttcctccgca tcgtccccag ccggaagcga
    38221 acagcgtgca gcgtaaacac atgcttgaac tggcgattgc cgacaagcca ttatttactc
    38281 ttgatgaacg cgagctaaag cgcaatgccc cctcttacac tgcgcaaaca ctgaaagagt
    38341 ggcggcagga acaagggccg gacgtgccgc tggcgtttat tattggtcag gattcattgc
    38401 tgacctttcc gacctggtac gaatacgaaa cgatactcga caatgcacat ttgatcgtct
    38461 gtcggcgtcc aggttaccca cttgaaatgg cgcaaccgca ataccagcaa tggctggaag
    38521 ataatttgac acataacccg gaagatcttc accttcagcc tgccggtaaa atttatctgg
    38581 ctgaaacgcc gtggtttaac atctcggcga ccatcatccg cgaacgtttg caaaacggtg
    38641 aatcgtgtga ggatttattg ccggaaccgg tactgactta cattaaccaa caaggcttgt
    38701 atcgctgata cctgtcgtta cggagggcat gatgcgactg tggttaattc gtcatggcga
    38761 aacgcaagcg aatgtcgatg gtctgtacag cggtcatgcg cccacacccc tgaccgcacg
    38821 cggtattgag caagcgcaaa atctgcatac gctgctacat ggtgtttcct ttgatctggt
    38881 tttatgcagt gaactggaac gggcacagca taccgcgcga ctggttctca gtgaccgcca
    38941 gctccccgtg caaatcatcc ctgaactcaa cgaaatgttt tttggcgact gggagatgcg
    39001 acatcatcgc gacctcatgc aagaagatgc cgaaaactat agcgcgtggt gcaatgactg
    39061 gcagcatgca atccccacga acggtgaagg atttcaggca ttttcgcaac gtgtggaacg
    39121 ctttatcgca aggcttagtg aatttcagca ctatcagaat attttagtcg tcagccatca
    39181 gggtgtactg agtctgttaa tcgcccgttt aattggcatg cctgccgagg ctatgtggca
    39241 ttttcgcgtt gaccaggggt gttggagcac cattgatatc aaccaaaaat tcgcgacgct
    39301 acgcgtcctc aatagccgtg ccatcggggt cgagaatgca tgacttttct gtttttttac
    39361 gggtaagccg caacgaccat tgacagaccc gggcaggctg atattctccg cagccagact
    39421 tttttcgcca gacacgactt tgtagaaatt gttttacaaa aatggcgatg caatctgcgg
    39481 cgcggggtgg gatgatagcc cactttcgaa agccgattcg gcgacaattg tcccgaaatc
    39541 gcctctggtt caggtatact gacagaccat ttttatctat ttgattcacc cagggggaaa
    39601 acttgcaggg taaagcactc caggattttg ttatcgacaa aattgatgac ctcaaaggtc
    39661 aggacatcat cgccttagac gttcaaggca aatccagcat caccgactgc atgatcatct
    39721 gtacgggtac gtccagccgt catgttatgt ccattgctga ccacgttgtg caggagtctc
    39781 gcgcagcggg cctgttaccg ctcggcgtag aaggtgaaaa cagcgccgac tggattgtcg
    39841 tggatttggg cgatgtgatt gtccatgtca tgcaggaaga gagccgtcgc ctgtatgaac
    39901 tggaaaaact ctggagttaa tgcgtgaagc tgcaacttgt cgccgtggga acgaaaatgc
    39961 cggactgggt acaaaccggt tttaccgagt acctgcgtcg ttttccgaaa gatatgccct
    40021 tcgagctgat tgaaattccg gccggaaaac gcggcaagaa tgcggacatc aagcgcatac
    40081 tcgacaaaga gggtgagcag atgttggcgg ccgcaggcaa aaaccgcatt gtcaccctcg
    40141 atattccagg caagccctgg gatacaccgc agttagccgc tgagctggaa cgctggaagc
    40201 tggatggtcg cgacgtcagt ctactgattg gcgggcctga agggttgtcg cctgcctgta
    40261 aagcggcggc ggagcagagc tggtcgctgt cggcgcttac cctcccccat ccgctggttc
    40321 gcgtgctggt cgcagagagt ctgtaccggg cgtggagcat caccaccaac catccttatc
    40381 accgtgagtg ataagggagc tttgagtaga aaacgcagcg gatgaaacta cagaactctt
    40441 ttcgcgacta tacggcagag tccgcgctgt ttgtgcgccg ggcgctggtc gcatttttgg
    40501 ggattttgct gctgaccggc gtgcttatcg ccaacctgta taatctgcaa attgttcgct
    40561 ttaccgacta ccagactcgc tctaatgaaa accgcattaa gctggtgcct atcgcgccca
    40621 gccgcggcat tatctacgac cgtaatggta tccctctggc cctcaaccgc actatctacc
    40681 agatagaaat gatgccagag aaagtcgata acgtgcagca aacgctggac gctttgcgca
    40741 gcgtggttga tctgaccgat gacgatattg ctgcattccg aaaagagcgc gcacgttcac
    40801 accgtttcac ctctattccg gtgaaaacta acctgaccga agtacaagta gctcgctttg
    40861 ccgtcaatca gtaccgtttt ccgggtgtcg aagttaaagg ctataaacgt cgttactatc
    40921 cttacggttc ggcgttgacc cacgtcatcg gctatgtgtc gaaaatcaac gataaagacg
    40981 tcgaacgcct gaataatgac ggcaaactgg ccaactatgc ggcaacgcat gatatcggta
    41041 agctgggcat tgagcgttac tatgaagacg tgctgcacgg tcagaccggt tatgaagagg
    41101 ttgaagttaa caaccgtggg cgtgttatcc gccagttaaa agaagtacca ccgcaagccg
    41161 gacacgatat ttacctgacg ctggatctca aactccagca atatattgaa acgctgctgg
    41221 cgggtagccg cgcagctgtg gttgtcaccg atccgcgtac aggtggggtg ctggcgctgg
    41281 tttccacgcc tagttatgac ccaaacctgt ttgttgacgg tatctccagc aaagattatt
    41341 ccgccttgtt gaacgacccg aatacaccgc tggtgaaccg cgccacacag ggggtttatc
    41401 ctcccgcgtc tacagttaaa ccctatgtgg cggtttcggc attgagcgcc ggggtgatca
    41461 cgcgcaatac gacgctgttt gacccaggct ggtggcaact gccaggttcg gaaaaacgtt
    41521 atcgtgactg gaaaaaatgg ggccacgggc gtctgaatgt cacaagatcg ctggaagaat
    41581 ctgcggatac cttcttctat caggtggcct acgatatggg gatcgatcgc ctctccgaat
    41641 ggatgggtaa attcggttat ggtcattaca ccggtatcga cctggcggaa gaacgttccg
    41701 gcaacatgcc tacccgcgaa tggaaacaga aacgctttaa aaaaccgtgg tatcagggtg
    41761 acaccattcc ggttggtatc ggtcagggtt actggacagc gaccccaatc cagatgagta
    41821 aggcactgat gatcctgatt aatgacggta tcgtgaaggt tccgcatttg ctgatgagca
    41881 ccgccgaaga cggcaaacag gtgccatggg tacaaccgca tgaaccgccc gtcggcgata
    41941 ttcattccgg ttactgggag ctggcgaaag acggtatgta cggtgttgct aaccgcccta
    42001 acggtacggc gcataaatac tttgccagcg caccgtacaa aattgcggcg aaatctggta
    42061 ccgctcaggt cttcggtctg aaagcgaacg aaacctataa tgcgcacaaa attgccgagc
    42121 gtttacgtga ccacaaactg atgaccgcct ttgcgccata caacaatccg caagtggctg
    42181 tcgccatgat tctggagaac ggcggcgcgg gtccggcggt tggtacgctg atgcgccaga
    42241 tcctcgacca cattatgctg ggtgataaca acaccgatct gcctgcggaa aatccagcgg
    42301 ttgccgcagc ggaggaccat taatcatgac ggataagccg aataaaaaaa cattctggga
    42361 taaagtccat ctcgatccca caatgctgct gattttactg gcattgctgg tttacagcgc
    42421 cctggttatc tggagcgcca gcggtcagga tattggcatg atggagcgca aaatcggcca
    42481 aatcgcgatg ggtctggtca tcatggtggt gatggcgcaa attcctccac gcgtttatga
    42541 aggctgggcc ccctatctct atatcatctg tattattttg ctggtggcgg tagatgcttt
    42601 cggtgccatc tctaaaggtg ctcaacgctg gctggacctc ggtattgttc gttttcagcc
    42661 gtcggaaatt gccaaaatag ccgtaccact gatggttgcg cgctttatca accgcgacgt
    42721 ttgcccgcca tcgttgaaga acactggcat cgcgctggtg ctgatattta tgcccacgct
    42781 gctggtggct gcacagcctg acctgggaac atcaatcctc gttgcgcttt ccggtctgtt
    42841 tgtactgttc ctctctggcc ttagctggcg tctgattggc gtcgcagtag tgctggtagc
    42901 ggcgttcatt ccgattctgt ggttcttcct gatgcatgat taccagcgcc agcgcgtaat
    42961 gatgctcctg gacccggaat cagacccact aggcgcgggc tatcacatta ttcagtctaa
    43021 aattgctatt ggctccggcg gattacgcgg caaaggctgg ctgcacggca ctcagtcaca
    43081 gcttgaattt ctccccgaac gccataccga ctttatcttc gcggtactgg cggaagagct
    43141 gggattagtg ggcattctga ttctgctcgc tctctacatt ctgctgatca tgcgcgggct
    43201 gtggatagcc gccagagcgc aaaccacctt tggtcgcgtc atggctggtg gcttaatgct
    43261 gatattattc gtttatgtct tcgtaaatat tggtatggta agcggtattc tgccggttgt
    43321 aggggttccg ctcccactgg tcagttatgg aggatcggcg ctaattgtgc tgatggctgg
    43381 gttcgggatt gtaatgtcaa tccacaccca caggaaaatg ttgtcgaaaa gcgtgtaaga
    43441 ggtgcgcaat gcgtaagcag tggctcggga tctgcatcgc ggcaggaatg ctcgcggcat
    43501 gtacaagcga tgatggtcag caacagacgg taagtgtacc gcagcctgcg gtatgtaacg
    43561 gccccatagt tgaaattagc ggggcggacc cgcgtttcga accactgaac gcgacggcaa
    43621 atcaggatta ccagcgcgac ggtaaaagct acaaaatcgt gcaggatccg tctcgattta
    43681 gccaggcggg actggcggca atctatgatg ccgaacctgg cagtaacctg acggcctctg
    43741 gcgaagcttt cgatccgaca cagttgacgg cggcccatcc aacgcttccg atccccagct
    43801 acgccagaat cactaacctg gctaacgggc gaatgatcgt ggtgcgcatt aatgatcgcg
    43861 gcccctacgg caacgaccgc gtaatttcgc tttctcgcgc agcagctgac cgtcttaaca
    43921 cgtcaaacaa caccaaagtt cgtatcgatc cgattattgt cgcccaggat ggttcgcttt
    43981 ctggtcctgg tatggcttgt accacagtcg ccaaacagac ttacgccctg cctgcacctc
    44041 ccgatttaag cggtggcgcg ggggcaagtt cagtgtctgg cccgcagggt gacattcttc
    44101 cggtcagtaa ctcgacgcta aaaagcgaag atccgaccgg cgcgccggta accagcagcg
    44161 gtttcctcgg cgcaccaacg accttagcgc ctggtgtact ggaaagcagc gaaccgacac
    44221 ctgcaccaca gcccgttgtt acagctccgt cgacaacgcc tgcaacctcg cctgcaatgg
    44281 tgacaccgca agccgcctcg caaagcgcca gcggcaactt tatggtgcaa gtcggggccg
    44341 ttagcgatca ggctcgtgcg caacagtacc aacagcaact gggacagaag ttcggcgtcc
    44401 ccggtcgcgt aactcaaaat ggcgcggtct ggcgtatcca gcttggccca ttcgccagta
    44461 aagccgaagc cagtaccttg cagcaacgtt tgcaaaccga agcccaatta cagtcattta
    44521 ttaccaccgc gcagtagcgt aaagcaggca tctgatgtgt caatttctgt aaatgacgtt
    44581 aacaaagtca cgcggaaagt cagatgcctg ccggtagtgc atttgctata gtagggcact
    44641 ttttttaatt ccatcacgga tgtcgtagtt cagaccatga ataccatttt ttccgctcgt
    44701 atcatgaagc gcctggcgct caccacggct ctttgcacag cctttatctc tgctgcacat
    44761 gccgatgacc tgaatatcaa aactatgatc ccgggtgtac cgcagatcga tgcggagtcc
    44821 tacatcctga ttgactataa ctccggcaaa gtgctcgccg aacagaacgc agatgtccgc
    44881 cgcgatcctg ccagcctgac caaaatgatg accagttacg ttatcggcca ggcaatgaaa
    44941 gccggtaaat ttaaagaaac tgatttagtc actatcggca acgacgcatg ggccaccggt
    45001 aacccggtgt ttaaaggttc ttcgctgatg ttcctcaaac cgggcatgca ggttccggtt
    45061 tctcagctga tccgcggtat taacctgcaa tcgggtaacg atgcttgtgt cgccatggcc
    45121 gattttgccg ctggtagcca ggacgctttt gttggcttga tgaatagcta tgttaacgcc
    45181 ctgggcctga aaaacaccca cttccagacg gtacatggtc tggatgctga tggtcagtac
    45241 agctccgcgc gcgatatggc gctgatcggc caggcgttga tccgtgacgt accgaatgaa
    45301 tactcgatct ataaagaaaa agaatttacg tttaacggta ttcgccagct gaaccgtaac
    45361 ggcctgttat gggataacag cctgaatgtc gacggcatca aaaccggaca cactgacaaa
    45421 gcaggttaca accttgttgc ttctgcgact gaaggccaga tgcgcttgat ctctgcggtg
    45481 atgggcggac gtacttttaa aggccgtgaa gccgaaagta aaaaactgtt aacctggggc
    45541 ttccgtttct tcgaaaccgt taacccactg aaagtaggta aagagttcgc ctctgaaccg
    45601 gtttggtttg gtgattctga tcgcgcttcg ttaggggttg ataaagacgt gtacctgacc
    45661 attccgcgtg gtcgcatgaa agatctgaaa gccagctatg tgctgaacag cagtgaattg
    45721 catgcgccgc tgcaaaagaa tcaggtcgtc ggaactatca acttccagct tgatggcaaa
    45781 acgatcgagc aacgcccgct ggttgtgttg caagaaatcc cggaaggtaa cttcttcggc
    45841 aaaatcattg attacattaa attaatgttc catcactggt ttggttaaaa attgaacact
    45901 tgaaagtgta atttccgtcc ccatatacta agcatcagta aaaaaactcc cgccttctgg
    45961 cgggagttgc tatttaatta cgttacgccg gagctgacat gaaaaccaaa cttaacgaac
    46021 tgcttgaatt ccctactcct tttacttaca aagttatggg gcaggcgtta cctgagctgg
    46081 ttgatcaggt ggttgaagtg gtacagcgcc atgcgccagg tgactacacc ccaacggtaa
    46141 aaccaagcag caaaggcaac taccactcgg tatctatcac tatcaacgcc actcatatcg
    46201 agcaggttga aacactgtat gaagaactgg gcaaaatcga tattgtccgc atggttctgt
    46261 aactcgcttc tccgttaccc ggctcctggt cgggtaactc ccctctcctc gctgtgatat
    46321 acttttcccc cacttttact cattctccac ggagatgccg ttttgtatca ggataaaatt
    46381 cttgtccgcc agctcggtct tcagccttac gagccaatct cccaggctat gcatgaattc
    46441 accgataccc gcgatgatag tacccttgat gaaatctggc tggtcgagca ctatccggta
    46501 ttcacccaag gtcaggcagg aaaagcggag cacattttaa tgccgggtga tattccggtg
    46561 atccagagcg atcgcggtgg gcaggtgact tatcacgggc cgggacaaca ggtgatgtat
    46621 gtgttgctta acctgaaacg ccgtaaactc ggtgtgcgtg aactggtgac cttgcttgag
    46681 caaacagtgg tgaataccct ggctgaactg ggtatagaag cgcatcctcg ggctgacgcg
    46741 cctggtgtct atgtcgggga aaagaaaatt tgctcactgg gtttacgaat tcgacgcggt
    46801 tgttcattcc acggtctggc attaaacgtc aatatggatc tttcaccatt tttacgtatt
    46861 aatccttgtg ggtatgccgg aatggaaatg gctaaaatat cacaatggaa acccgaagcg
    46921 acgactaata atattgctcc acgtttactg gaaaatattt tagcgctact aaacaatccg
    46981 gacttcgaat atattaccgc ttaattccat acatcaatgg cccaatttac actgggtcat
    47041 tactcataat acctgctaat gctttacatc tccttcgcgt ttgtatattt tctttatcac
    47101 ttgcagaaat gcggtgccac gccttgctcc gtaactcacc ccattgatta acctattgaa
    47161 atagttgatt atggtgagca aaagcgttcc gttccaatta gacataagat tattgtaatt
    47221 ttttaaatat tatttttcca tgaataaaat tggagtaagt gcgtggatag taataatcaa
    47281 attgaaccct gtttaagcag aaagtcgtca gaaggtaaac cacaaatatt tacaacctta
    47341 cgaaatattg atcttaacct tctgactatt tttgaagctg tatatgtaca taaagggatc
    47401 gttaatgcag cgaaagtgct taatctgacc ccctcggcaa tcagtcagtc tattcagaaa
    47461 ctgcgcgtta tattccctga cccattgttt attcgcaaag gccagggtgt cactcctacc
    47521 gcatttgcga tgcatctaca tgagtatatc agtcagggcc ttgagtccat tcttggcgcg
    47581 ctggatatcg aaggaagcta tgataagcaa caaacgataa ctattgcgac cactccctcg
    47641 gtcggagccc tggtccttcc tgtcatctat cgggcgatta aaactcacta tccgcagctt
    47701 ttactgcgca acccgcccat cagcgacgcg gaaaaccaac tcagtcagtt tcaaaccgat
    47761 ctcatcatcg ataacatgtt ttgcaccaat cgtacggtgc aacatcatgt tctgttcacc
    47821 gacaatatgg tgttaatttg ccgcgaggga aatccactac tctctttaga agatgacaga
    47881 gagactatcg acaacgctgc gcatgtactt ctgttaccgg aagagcaaaa tttcagcggt
    47941 ctgcggcaga gagttcaaga aatgtttccg gaccggcaaa tcaatttcac cagctacaac
    48001 attttgacaa tcgctgcact ggttgccaac agtgacatgt tagcgattat tcccagccgt
    48061 ttttataacc tgtttagccg ctgctggccg ctggaaaaac tgccttttcc gtccttaaat
    48121 gaggagcaaa tagatttctc tatccactac aacaaattca gcctgcgtga tcccatcctg
    48181 cacggagtaa tagatgttat ccgtaatgca ttttgaaaaa gtattgttac agccagatca
    48241 cagcgcagaa cgataaaatg gcacaatcca acaacaattt tacattttcg cgaccgcttt
    48301 ggctgctttc aggtccgttt caatgatata ctgccagtcg ttaattcaaa aatagttgat
    48361 aattacaaca atctattgaa ttgaaacgct ttccttcgta attcgcaact ggaacacgca
    48421 cgctatgagt aaacccattg tgatggaacg cggtgttaaa taccgcgatg ccgataagat
    48481 ggcccttatc ccggttaaaa acgtggcaac agagcgcgaa gccctgctgc gcaagccgga
    48541 atggatgaaa atcaagcttc cagcggactc tacacgtatc cagggcatca aagccgcaat
    48601 gcgcaaaaat ggcctgcatt ctgtctgcga ggaagcctcc tgccctaacc tggcggaatg
    48661 cttcaaccac ggcacagcaa cgtttatgat cctcggcgct atttgtaccc gccgttgtcc
    48721 gttctgtgat gttgcccacg gtcgcccggt agctcctgat gccaatgaac cagtgaaact
    48781 ggcgcagacc attgccgata tggcgctgcg ttatgtggtt atcacctccg ttgaccgtga
    48841 tgacctgcgc gatggcggtg cccagcactt tgcggattgc attactgcca ttcgggaaaa
    48901 aagcccgcaa atcaaaattg aaactctggt gccggatttc cgtggtcgta tggatcgtgc
    48961 tctggatatt ctgactgcaa cgccaccaga tgtgttcaac cataacctgg aaaacgtacc
    49021 gcgtatttac cgtcaggtac ggcctggtgc agattacaac tggtcgctga agctgctgga
    49081 acgctttaaa gaagcgcatc cggaaatccc gaccaagtct ggtctgatgg tgggactggg
    49141 tgaaaccaat gaagaaatta ttgaggtaat gcgcgacctg cgccgtcatg gtgtgacgat
    49201 gttaacgctg gggcaatatt tgcagccaag ccgccatcac ctgccggttc aacgttacgt
    49261 tagcccggat gagttcgacg aaatgaaagc cgaagcgctg gcgatgggct ttacccatgc
    49321 tgcatgcggt ccgtttgtcc gctcttctta ccacgccgat ttgcaggcga aagggatgga
    49381 agttaagtaa tcctgtaata attacttaca tctgtctgat aaaaaacccg ctttttgaag
    49441 cgggtttttt gtatcgaaca agatattgag ggagcgtcct gctcgccacg tcactcttta
    49501 tgagagagct tttcagcctg aagatcaacg tctgcgcctt ttttcgccgc agcatcgtca
    49561 tcattcatcg ccttcttgaa ccctttaatg gccgctccaa ggtctccgcc cagcgtacgt
    49621 aacttcttag tcccaaacag cagaacgacc agcgccgcaa ctaccagcag tttggtaata
    49681 ctaatctcac ccatagatac cttcttgaca tataaccggc aacagaacgc gccttaaaaa
    49741 ccagagaata ttaacggtca ttcgacgcgc gcacacaata gcaatctgta acaaggtgaa
    49801 tcaagcgccg agtagaaaaa catcataata attgcggcgg cgcaaagcga cggttgttta
    49861 agacgggcag ttgcgcgcgc acctgacgca cacgttcggg cgtcacttcc gccataatga
    49921 gtgcaggcat ttctgacgct gccgcaatgg tgacgccaaa gggatctata atccggcttt
    49981 gaccgatatt tttgttcccg cactcccccg ccgccaccat ataacaggtg gtatccagcg
    50041 cacgagcggc aagcaacgtt gaccagtgat gctctttgag cggcccgcga acccaggcgg
    50101 caggaagtac caggatttca gctccctgta atgcctgtgc cagcgccagc tctggaaagc
    50161 gtaagtcata acaggtcatc agaccgacct tcatcccttc cacctccagt aacggagcga
    50221 tttcattacc agcatcaaca cggcgtgatt cctgaatggc aaatgcatca tagagatgca
    50281 gtttggcata acgggcgacg atgtttcctg cctgaagtgc caccagcata ttccatgcgc
    50341 gccccggcgt tgaaggaaca tgaatcgtca gaattgtcgt catcatgtta cgtttacttt
    50401 ctcgccgtaa acgtccgagg aattcgcctt ccagcagctg tgctgattta accgatagat
    50461 ctgcatcatg atcatcgcgc gccagcaatg cttccggcag gacaaacagc gatacgtcgt
    50521 tttccgccgc ctgcgccatc aacgaggcac aaatctcagc gttcttttcc cacacagatg
    50581 taacagcaaa ctgtcctgcg gcaaccagca ttgccacctc ctgtcagagt cgtaaaattc
    50641 gccaacgacg attattcggc gttacactta tcactcatac aaatcaaata gcaggatttt
    50701 gcagtgttac aacttctttt agcagttttt attggcggtg gtacgggaag cgtggcgaga
    50761 tggctgttaa gtatgcgatt taacccactg catcaggcga ttccgttggg gacgctgaca
    50821 gcaaacctga ttggggcatt catcatagga atgggattcg catggttcag caggatgacg
    50881 aacattgatc cagtgtggaa agtattaatc accaccggat tttgtggcgg tctaacaacc
    50941 ttctcaacat tttcggcaga agtggtgttt ttgttacaag agggccgctt tggctgggca
    51001 ttactgaacg ttttcgtcaa ccttctgggg tcttttgcca tgaccgcact ggcattctgg
    51061 ctgttttcgg cctcaaccgc acactaaaga ccaaaaaaaa cccgctgatt aagcgggttt
    51121 tgaattcttg ctgacgtatc ttacagagcg attacgtttg cagcagaagg gcctttggca
    51181 ccgttagtga tttcgaactc tacgcgctga ccttcagcaa gagttttaaa accattagtc
    51241 tggattgcag agaagtgtac gaacacgtct ttgctgccgt cttccggagt aatgaaaccg
    51301 aatcctttgg actcattaaa ccacttaacg ttacctttaa tcttagacat caaaattacc
    51361 tttacatgaa aaatagacac aaatgctgtg tcggttacca gtacaccaat tgtggtacgc
    51421 tttgtccagt gcaactttac taaaaagtga taaaagtcgc gttatttttg atgttactta
    51481 ttttagctat tgattttaaa gaagttacta aaacttcatt tgcctcaaaa ctgaaagcgc
    51541 atccaggcaa agtacacatt gccattgttg taggtacccg gaatgtaggt catctgaaaa
    51601 gtcactgggc cataacccac ggaggccaat ggcagtagaa ccgggagagg gatgtaattc
    51661 cagttatcgc gtgccgttac gccagcggtg aatcccagac ctaaatgaaa attttcatcc
    51721 gccagcggtc gccaggtact ttcccatccg tatccggcaa tcggttccca tttgttccac
    51781 gagtccttaa atgccatggc atacaggcca tgccagtttc ctttttcatc ccaacgcgac
    51841 aggccaaaac cgccacccca cggtcgctcg ttatagcgat cggttttttc tttgtcgtaa
    51901 gcgaaacgtg catgccaggt gatggcagga atatataaat cataatgttc aggctgttgc
    51961 caggtttgtg caatattttc tctaaacgtt gtcatccact catctgcgtt agcaaaaact
    52021 ttaccaacgc tgattaactg aataaaaaca aaggaaaaga tagcgacata tttactcacg
    52081 ttcatttgtg accataaaac atttatcaaa aatctactac tagcatagca aagctacaat
    52141 taacataacc ttaatagacc caacataaag aataatctga atagctgctc tatttatttc
    52201 cgcttgttca agttaaatat ttccgcgcat cgattaaaga tgatgcgtcg ttttctgcga
    52261 tgggaatagt caaaaaagaa aaaccaagtc ttttttgatg acaaaatgca atcaaggaaa
    52321 aattatttta ttttttaacg agatacaaca atcatcttaa cgaagtatat aatatatatc
    52381 gtcaacctat gctcatatta gattattttt tgagcgcaca gccgcagcta acaccattgc
    52441 aattaacaaa tttgcatcaa tccaccatca atttgcacac attattatgt gataattgcc
    52501 aaccgctaaa tatgcgtttt gttatctatg tataaaaaca gcaacttcaa tgtcttaatg
    52561 gcagtttttc ttgattttaa tcagcattca tcgccaattt attgggcata ttttttcctt
    52621 aagctttagg aattttttat tatttacttt ggggcctgga gacaggaaaa attatgctga
    52681 cattcattga gctccttatt ggggttgtgg ttattgtggg tgtagctcgc tacatcatta
    52741 aagggtattc cgccactggt gtgttatttg tcggtggcct gttattgctg attatcagtg
    52801 ccattatggg gcacaaagtg ttaccgtcca gccaggcttc aacaggctac agcgccacgg
    52861 atatcgttga atacgttaaa atattactaa tgagccgcgg cggcgacctc ggcatgatga
    52921 ttatgatgct gtgtggattt gccgcttaca tgacccatat cggcgcgaat gatatggtgg
    52981 tcaagctggc gtcaaaacca ttgcagtata ttaactcccc ttacctgctg atgattgccg
    53041 cctattttgt cgcctgtctg atgtctctgg ccgtctcttc cgcaaccggt ctgggtgttt
    53101 tgctgatggc aaccctattt ccggtgatgg taaacgttgg tatcagtcgt ggcgcagctg
    53161 ctgccatttg tgcctccccg gcggcgatta ttctcgcacc gacttcaggg gatgtggtgc
    53221 tggcggcgca agcttccgaa atgtcgctga ttgacttcgc cttcaaaacg acgctgccta
    53281 tctcaattgc tgcaattatc ggcatggcga tcgcccactt cttctggcaa cgttatctgg
    53341 ataaaaaaga gcacatctct catgaaatgt tagatgtcag tgaaatcacc accactgctc
    53401 ctgcgtttta tgccattttg ccgttcacgc cgatcatcgg tgtactgatt tttgacggta
    53461 aatggggtcc gcaattacac atcatcacta ttctggtgat ttgtatgctg attgcctcca
    53521 ttctggagtt cctccgcagc tttaataccc agaaagtttt ctctggtctg gaagtggctt
    53581 atcgcgggat ggcagatgcg tttgctaacg tggtgatgct gctggttgcc gctggggtat
    53641 tcgctcaggg gcttagcacc atcggcttta ttcaaagtct gatttctatc gctacctcgt
    53701 ttggttcggc gagtatcatc ctgatgctgg tattggtgat tctgaccatg ctggcggcag
    53761 tcacgaccgg ttcaggcaat gcgccgtttt atgcgtttgt tgagatgatc ccgaaactgg
    53821 cgcactcttc cggcattaac ccggcgtatt tgactatccc gatgctgcag gcgtcaaacc
    53881 tgggtcgtac cctatcaccc gtttctggcg tagtcgttgc ggttgccggg atggcgaaaa
    53941 tctcaccatt tgaagtcgta aaacgcacct cggtgccggt gcttgttggg ctagtgattg
    54001 ttatcgttgc tacagagctg atggtgccag gaacggcagc agcggtcaca ggcaagtaaa
    54061 tagtaatgcc ggaggcaagt ttcctccggc tttatcatgt cacccactgt ggtatatgcg
    54121 ttgtggtctg ccaactttgc cgtgaacaat ttcagcaata atcagatggc ggctggcgca
    54181 atattcaaga taacgcctgg cagtggtgcg actgatggtc agtgcctgcg ccaccgtttc
    54241 tgccgtatgt tgcacaccag gctctttaaa cagttttcgc accgcgttta gcgtcaaggg
    54301 atcaatgccg gtcggcagtt cgtctttagg ttcaccgcga gcataggcat taaacatctc
    54361 atcaatttgc ttctggctgg cgctatcaat actttccagc atatgtttac gctggcggaa
    54421 acgggttagc gtttgcccca gccgttcata ggcaatgggc ttaatgagat aatcaaatac
    54481 accacaacgt acggcttcag acaccgtttc catatcgctg gctgcagtgg taaacaccac
    54541 gtcgccggga taatgcgcct gcaccagttc atgcaataaa ttaatccctc tgccgtcagg
    54601 aagatagtta tcgagcaaga ttagccccgg cttaaaacgc tcgatcatca ttcgggcctg
    54661 cgccaggttt cccgccagta atatctgact gaatccggga atgtgacgaa tatattccgc
    54721 atgcatctct gccagcggcg tttcgtcctc aacgatcaat agggttaatg gagctgtcat
    54781 taggtttcac tttcggaata tagattgaaa acaaggtacc gcagggatca ttatcttcga
    54841 gagtgataac accaccgcag cgcgttacgt agctggcaat caagtacaac ccaatgccat
    54901 gttcaccggg ctcgtcagca cgcgtactga ccccctgctc aaatattttg tctcgtagag
    54961 actctggaac gccgcagccc tgatcggcga cttcaatcac cacatcatcg ccttcatcgc
    55021 tgaggaataa ttcaacgatc ttgtttcctt catcgctacg caggctggct tcgaaggcgt
    55081 tatcaagtaa attgcccaca atggctgcaa actcggtgct atccagtcct ggcggcagtt
    55141 gcgaaagctg gctaccgggg acaatgatca tttttagccc cagttcccgg gcgcgctgca
    55201 ctttaccaaa aagcagcccc gccacctggc gatcggcaaa cgcctcacgc aggctgtcaa
    55261 taagctgttg ctgggcctga gactccccct gcaccatcgc cagcacgcga tcatactctt
    55321 tcatctgcaa cagaccattg agcgtcgaca tccaattgag atgctcgtgt cgcaatgtac
    55381 gaaggctctc gacgtattgt tttatttgcg tcagttgcgc attgagggtg gaaatttcgt
    55441 ctttactacg aaagctgata atggccccca gcaaatcatc acctgaacga atagcttccc
    55501 gattggcaat aacgctcaga ccgttaaagt tagccaccac atcctgacgt ttttcatcaa
    55561 tctgttcggt aaagaaatcg gcgggcctga ccacttcagc aatgggttta cccaaccatt
    55621 gccgtccggg agagctcagc cccagcatct ttcttgcgtt acgattgatg gcggtaatgt
    55681 aaccatgcgg atccaccgca atcagccctt cataaaccga actaaacagc gcctcttgct
    55741 gacggaccac gcgtgcgatt tgctttggct ccatgcccat catttgccga cggatatgcg
    55801 ctgccaggaa ccacgacagc aacatcagaa tccctaacag cacgacaaac acgcctgcca
    55861 tcggtaataa aaactcagcc cgccagctat cgattttact caccaggtag ccaatcgaca
    55921 ccacgccgat gacttttcca tcgtcatcaa agattggcgt tttggcgcgc atcgccatcc
    55981 ccattgaccc tttcccggta atgaagtagc tctccccttt ctccagcgcg ccctgtttgg
    56041 tgaactgcat aggataacca attttctccg gattaggatg gtaaaggcgg atcgagtgcc
    56101 ggtccccaat caccacataa tcaaaatcgg tatctctttg taatttgtta gcgatggtcg
    56161 ccagccgttt gtagtcacgc gttttcaccg cagagatgac actgtcattg gaggcaataa
    56221 ttttcgcctg attcattgcc atgtcgcgta catgaagcgt taaatagtcc tcaaaactgg
    56281 ccgtaaaata ttgcgccagg gctgcaatga caaatattga gaacaccaga atcagcaaaa
    56341 agatacgcag cggaaatgcc agtctttgga aaaatgcaaa ctgtttattc tcgttaagct
    56401 gcaacattgt tttccttgcg ggttgtcccg agcctctttg acggatgtaa ttaaaatgtt
    56461 gaatcaaaat acatagatat taattatgat cgtggcaaat tttattcatt aaatcaatta
    56521 aatcacttaa acctgcaggt catttcgtaa ttacattaat taaaaaactt aaaaccatta
    56581 aataaataaa aaaccactaa ctctatgtga aataaatcaa aatttcacgc agaaatactc
    56641 cttaggatgt atagcgaaag gagaaaaaga tatacctcga tcacccccct ttctcccaag
    56701 tgaaaataaa aggttatcag tttgcaacat tgaacaacat tcgttgcaaa tcgataacaa
    56761 catgcacctt caggatacta tttattatgt tcggcaatga tattttcacc cgcgtaaaac
    56821 gttcagaaaa taaaaaaatg gcggaaatcg cccaattcct gcatgaaaat gatttgagcg
    56881 ttgacaccac agtcgaagta tttattaccg taacccgcga tgaaaagctt atcgcgtgcg
    56941 gtggaattgc cggaaatatt attaaatgcg ttgcgatcag tgaatccgtt cgcggtgaag
    57001 gactggcgct gacattagcc actgaactga taaacctcgc ctatgagcgg cacagcacgc
    57061 atctgtttat ttataccaaa accgaatacg aggcgctgtt ccgccagtgc ggtttttcca
    57121 cgctgaccag cgtacccggc gtgatggtgc tgatggaaaa cagcgccacg cgactgaaac
    57181 gctatgccga atcgctgaaa aaatttcgtc atccagggaa caagattggc tgcattgtga
    57241 tgaacgccaa tcccttcacg aatggtcacc gttatctgat tcaacaagct gcggcacagt
    57301 gcgactggtt gcatctgttt ttagtcaaag aagattcttc acgcttcccc tatgaagacc
    57361 ggttggatct ggtgttaaaa ggcaccgccg atattccacg cctgactgtg catcgcggct
    57421 ccgaatacat catctcccgc gctacgttcc cttgctactt cattaaagaa cagagcgtca
    57481 ttaaccattg ttacaccgaa attgatctga agattttccg tcagtacctc gctcccgcgc
    57541 tgggtgtaac tcaccgcttt gtcggtactg aacccttttg tcgcgttacc gcccagtaca
    57601 accaggatat gcgctactgg ctggaaacgc cgactatctc cgcaccgccc atcgaactgg
    57661 ttgaaattga gcggctgcgt taccaggaga tgccgatatc cgcttcccgg gtacgtcaac
    57721 tgctggcgaa aaacgatctc acggctatcg cgccgctggt ccctgcagtc acgctgcatt
    57781 atttgcagaa cctgcttgag cactcccgcc aggacgcggc agctcgtcaa aagacccccg
    57841 catgagaaac aggtgaaaaa tgaaaataaa ccagcccgcc gttgcaggca cccttgagtc
    57901 tggggatgtg atgatacgca tcgccccact cgatacgcag gatatcgacc tgcaaatcaa
    57961 tagcagcgtt gagaaacagt ttggcgatgc aattcgcacc accattctgg acgttctcgc
    58021 ccgctacaac gtgcgcggcg tacagctgaa tgtcgatgat aaaggcgcac tggactgcat
    58081 tttacgtgca cgactggaag ccctgctggc acgcgccagc ggtatcccgg ctctgccatg
    58141 ggaggattgc caatgatttc cgcttcgctg caacaacgta aaactcgcac ccgccgcagc
    58201 atgttgtttg tgcctggtgc caatgccgcg atggtcagca actccttcat ctacccggct
    58261 gatgccctga tgtttgacct cgaagactcc gtagcattgc gtgaaaaaga caccgcccgc
    58321 cgcatggttt accacgcgct gcaacatccg ctgtatcgcg atattgaaac cattgtgcgt
    58381 gtcaacgcgc tggattccga atggggtgtc aacgacctgg aagccgtcgt tcgcggtggt
    58441 gcggacgttg tgcgtctgcc gaaaaccgat accgctcagg atgttctgga tatcgaaaaa
    58501 gagatcctgc gtatcgaaaa agcctgtggt cgtgaacccg gcagcaccgg cctgctggcg
    58561 gcgattgaat ctccgctggg cattacccgc gcagtggaaa tcgctcacgc ttccgagcgt
    58621 ttgatcggta tcgccctcgg tgcagaagac tatgtgcgca acctgcgtac agaacgctcc
    58681 ccggaaggaa ctgaactgct gttcgcacgc tgttccattt tgcaggccgc gcgctctgcg
    58741 ggtattcagg cgttcgatac cgtctattcc gacgctaaca acgaagccgg attcctgcaa
    58801 gaagccgccc acatcaaaca gctgggcttt gacggcaaat cgctgatcaa cccgcgtcag
    58861 attgatctgc tgcacaacct ctacgcaccg acccagaaag aagtggatca cgcccgccgc
    58921 gtcgtagaag ccgctgaagc cgccgctcgc gaaggcctcg gcgtggtttc cctgaacggc
    58981 aagatggtgg acggtccggt tatcgatcgc gcccgtctgg tgctctcccg tgcagaactt
    59041 tccggcatcc gcgaagaata aggcaatcaa aatgacgcag aaaattgaac aatctcaacg
    59101 acaagaacgg gtagcggcct ggaatcgtcg cgctgaatgc gatcttgccg ctttccagaa
    59161 ctcgccaaag caaacctacc aggctgaaaa agcgcgcgat cgcaaactgt gcgccaacct
    59221 ggaagaggcg attcgtcgct ctggtttaca ggacggcatg acggtttcct tccatcacgc
    59281 tttccgtggc ggtgacctga ccgtcaatat ggtgatggac gtcatcgcga agatgggctt
    59341 taaaaacctg accctggcgt ccagctccct gagtgattgc catgcgccgc tggtagaaca
    59401 cattcgccag ggcgtggtta cccgcattta tacctctggc ctacgcggtc cactggcgga
    59461 agaaatctcc cgcggtctgc tggcggaacc ggtgcagatc cactctcacg gcggtcgtgt
    59521 gcatctggta cagagcggcg aactgaatat cgacgtggct ttcctcggcg tcccgtcctg
    59581 tgatgaattc ggtaatgcca acggctacac cggtaaagcc tgctgcggct ccctcggcta
    59641 tgcaatggtt gatgccgaca acgcaaaaca ggttgtgatg cttaccgaag aactgctgcc
    59701 ttatccgcat aatccggcaa gcattgagca agatcaggtt gatttaatcg tcaaagttga
    59761 tcgcgttggc gatgctgcaa aaatcggcgc tggtgctacc cgtatgacca ctaacccgcg
    59821 cgaactgctt attgcccgta gcgctgcgga tgtgattgtc aactcaggct acttcaaaga
    59881 aggtttctcc atgcaaaccg gcaccggcgg cgcatcgctg gcggtaaccc gtttcctgga
    59941 agacaaaatg cgtagccgcg atattcgcgc cgacttcgcc ctcggtggta ttaccgcgac
    60001 gatggttgat ctgcacgaaa aaggtctgat ccgcaaactg ctggatgtgc agagctttga
    60061 cagccatgct gcgcaatcgc tggcccgtaa ccccaatcac atcgaaatca gcgccaacca
    60121 gtacgctaac tggggttcga aaggcgcatc ggttgatcgt ctcgacgtgg tggtactgag
    60181 cgcgctggaa attgacaccc agttcaacgt taacgtgctg accggctctg acggcgtact
    60241 gcgtggtgct tccggtggtc actgcgatac cgcgattgcc tctgcgcttt ccatcatcgt
    60301 cgcgccgctg gtacgcggtc gtattccgac tctggtggat aacgtgctga cctgcatcac
    60361 cccgggctcc agtgtcgata ttctggtcac agaccacggt atcgcagtta acccggcacg
    60421 tccggaactg gcagaacgtc tgcaggaagc gggcattaaa gtggtttcca ttgagtggct
    60481 gcgcgaacgt gcgcgtctgc tgaccggtga accacagccg attgaattca ccgaccgcgt
    60541 cgttgccgtt gtgcgttacc gcgatggctc ggtgatcgat gttgtgcatc aggtgaagga
    60601 ataagccatg cacctgcttc ctgaactcgc cagccaccat gcggtgtcaa ttcccgagct
    60661 gctcgtcagc cgggatgaaa ggcaagcacg gcaacacgtc tggctcaagc gccatcctgt
    60721 tccactggtc tcctttaccg tggttgcgcc tgggccgatt aaagacagcg aggtcacacg
    60781 ccgaattttt aatcatggcg tgacagcctt gcgtgcctta gccgcaaaac agggctggca
    60841 aattcaggag caggctgcac tggtttccgc cagcgggccg gagggcatgt tgagcattgc
    60901 cgccccggct cgcgacctca agctcgccac cattgagctt gaacatagtc atcctctcgg
    60961 gcggttatgg gatatcgatg tcctgacgcc cgaaggcgaa attctctccc gccgcgacta
    61021 ttcactgccg cctcgccgct gcctgttgtg cgagcaaagc gcagccgtct gcgcgcgtgg
    61081 aaaaacccat caactgaccg atttactcaa ccgcatggag gcactgctga acgatgtcga
    61141 tgcctgcaac gtcaactaaa accacaaagc ttgcgacgtc attaatcgat gagtacgccc
    61201 tgctgggctg gcgcgccatg ctgactgaag tcaatctgtc accgaaacca ggcctcgtgg
    61261 atcgcattaa ctgcggtgcg cacaaagata tggcgctgga agatttccac cgcagcgcgc
    61321 tggcgattca gggctggtta ccccgtttca ttgaatttgg tgcctgtagt gcagaaatgg
    61381 caccggaagc ggtactccac ggattacgcc caattggtat ggcttgcgaa ggtgatatgt
    61441 tccgcgccac tgcgggcgta aacacgcata aaggcagcat tttttcttta gggctgctat
    61501 gtgcggcaat tggccgtttg cttcagctca accagccggt aacgccaaca accgtttgtt
    61561 ctacggcggc aagtttctgc cgtggcctga ccgatcgcga actgcgtacc aataattcac
    61621 aactgacggc aggtcaacgg ttgtaccagc agcttggcct taccggcgca cgcggtgaag
    61681 ccgaagcggg ttatccactg gtgatcaatc acgccttgcc gcattacctc actctgctgg
    61741 atcaggggtt agatcctgaa ctggcattgc tcgatacctt gctcctactg atggcgatca
    61801 acggcgatac caacgttgca tcgcgcggtg gcgagggggg cctgcgctgg ctacagcgcg
    61861 aggcgcaaac attattgcaa aaagggggca ttcgaacccc cgccgatctc gattatctcc
    61921 ggcagttcga cagggagtgt atcgaacgaa atctcagtcc aggcggcagt gccgacctgc
    61981 tgatccttac ctggttttta gcacagattt aattatttaa gcacttgata aatttggaaa
    62041 tattaatttt cggagaaccc gtatgtcttt agcaaaagat aatatatgga aactattggc
    62101 cccactggtg gtgatgggtg tcatgtttct tatccctgtc cccgacggta tgccaccaca
    62161 ggcatggcat tacttcgctg tgtttgtggc aatgattgtc ggcatgatcc tcgagccaat
    62221 tccggcaaca gcgatcagtt ttattgcggt tactatttgc gttattggca gtaattacct
    62281 gctctttgat gccaaagaat tagctgaccc agcgtttaat gcgcaaaaac aggcgctgaa
    62341 atggggtctg gctggttttt ccagcaccac ggtatggctg gtatttggcg catttatttt
    62401 tgcattaggg tatgaagttt ccgggttagg tcgtcgcatt gcccttttcc tggtgaaatt
    62461 catgggcaaa cgcacgctga cgttgggtta tgcgattgtc attatcgaca ttctgctggc
    62521 accgtttaca ccgtccaaca ccgcgcgtac cgggggtacg gtttttccgg tcattaaaaa
    62581 cctgccgccg ctgtttaaat cattccccaa cgatccgtcc gcgcgtcgta ttggcggcta
    62641 tttgatgtgg atgatggtca ttagtaccag tctgagttcg tccatgtttg tcaccggcgc
    62701 ggcaccaaac gtgctgggtc tggagttcgt cagcaaaatt gccggtatcc agattagctg
    62761 gttgcagtgg ttcctctgct tcctgccggt tggggttatc ttgcttatca ttgcgccgtg
    62821 gctttcctac gtgctgtaca aaccggaaat cacacacagt gaagaagtgg caacctgggc
    62881 gggtgatgaa ctaaaaacca tgggtgcgct gacacgcaga gagtggacgc tgattggcct
    62941 tgtattgctc agcttaggtt tgtgggtatt tggcagtgaa gtcattaatg ctactgcggt
    63001 tggtctgctg gcagtttcgc taatgctggc cctgcacgtt gtgccttgga aagatattac
    63061 ccgctataac agcgcatgga acacgctggt caacctggca actctggttg tgatggctaa
    63121 cggcctgact cgttctggct ttattgactg gttcgccggt accatgagta cgcacctgga
    63181 aggattctca ccaaacgcaa cggtgattgt actggttctg gtgttctact ttgcacacta
    63241 cctgtttgcc agcctgtctg cgcacaccgc aaccatgctg ccggttattc tggccgtcgg
    63301 taaaggtatt ccgggcgtac caatggaaca actgtgtatc ctgctggtgc tgtctatcgg
    63361 tatcatgggc tgtctgacgc cgtatgcaac cggtcctggg gtgattattt acggctgtgg
    63421 ctatgtgaaa tcaaaagatt actggcgtct tggcgcaatc ttcggggtga tttacatctc
    63481 tatgttgctg ttggttggct ggccgattct cgccatgtgg aactaatacg ttcgccaaac
    63541 gctgaataaa tcatcacgcc cgccaggtaa gcccacctgg cgggcttttt tatgatttaa
    63601 tagatagtat tcagtgtatg tatgagttcc acacccatta tgaaagcatt ctggcgtaac
    63661 gccgcgttgc tcgcggtttc tctgcttccc ttctcttctg ccaacgcctt agcgttgcag
    63721 gcaaaacagt atggcgattt tgatcgctat gtcctggccc tctcctggca aaccggattt
    63781 tgccagagtc aacacgatcg aaatcgtaac gaacgagatg aatgtcgcct gcaaaccgaa
    63841 acgaccaaca aagctgattt tctgaccgta catggtctgt ggccaggatt gcctaaatcg
    63901 gttgctgccc gtggtgttga tgaacgccgc tggatgcgct tcggttgcgc tactcgccca
    63961 atcccgaatc taccagaagc gcacgccagc cggatgtgtt catcgccgga aaccggatta
    64021 tcactggaaa cggccgctaa actaagtgaa gtcatgccag gagctggcgg acgttcctgc
    64081 ctggaacgct acgaatatgc caaacacggt gcctgctttg gttttgatcc ggacgcatac
    64141 ttcggtacga tggtacgcct gaatcaagaa attaaagaga gcgaagccgg aaaattcctt
    64201 gcggataatt acggtaaaac agtgagccgc cgtgactttg acgccgcctt tgccaaaagc
    64261 tggggaaaag agaacgtgaa agcagttaag ctaacgtgcc agggtaaccc tgcgtatttg
    64321 actgaaattc agatctcgat caaagctgac gccatcaacg ctccgctttc tgcaaactca
    64381 ttcttgccac aaccacaccc aggcaactgt ggcaaaacct ttgtgattga taaagtgggt
    64441 tattaatcat ctgatttaca aaacacgcta attcgtcttc cataaaccgt gacgattaag
    64501 aattatacat aatgcgctgc attgaggaat tccctgaatg tgacgcaaat cacttcaaag
    64561 agtgagcaac agtcagtatc atcatgtgag ttaaaccctc gccgcctgac ggtgagggtt
    64621 ttcttttggg tatgtttatc tgcgacactc gcagtaccga cgacatggag taaaaatgtc
    64681 cagaccaact atcatcatta acgacctgga tgccgaacgc atcgatattc tgctggagca
    64741 acccgcctat gctggtttgc caatcgccga cgcgttaaac gcagagttgg atcgcgccca
    64801 aatgtgttcg ccagaagaga tgccacacga cgtggtgaca atgaacagcc gggttaaatt
    64861 ccgcaatctt agcgatggcg aagtgcgtgt gcgcacgctg gtgtatccgg caaaaatgac
    64921 cgatagcaat actcagcttt ccgttatggc tccggtaggt gccgcactgc tggggctgcg
    64981 cgttggcgat tccattcact gggaacttcc gggcggcgtt gcaacccacc ttgaagtgct
    65041 ggaactcgaa taccagccag aagctgctgg cgactacctg ctttaatccc atctgcacaa
    65101 gcgataactc gctttgccca gaggatgcac tcgcatcctc tttacgcaaa atcagcaatt
    65161 tttcctggtt ctcactgttc acttttgctg caaaattatt ccgtttcgtt tgtgggaata
    65221 accatggcag aagcatttta catcctgata ggatttctga ttatggcggc gattatcgtc
    65281 atggctgtcc tttacctcga aaaccactcc tgacgatcat attgttcccc ccggcatcgc
    65341 attcaccaga cctgaaaccg ccttctgcgc cgcctctgcg ctttgtgcac cgggtaccag
    65401 aatcacctta cggcactcct cttcacgttt ttcgaaaatc tcatatcccc gggcggcctc
    65461 ttcaaacggc atatagtggg taacaatttc ttctggtttc agtaatcctt tctcaattaa
    65521 cggtaataat tctcccagcc atgcgtgaac gtgggtctgt cccattttaa acgtcaaccc
    65581 tttatcaaag gcgtcgccaa acaggaaacc gtgaataaat ccagcgtaga cgcccggtac
    65641 gctaacaatg ccgccacgcc tgaccgccgc aatacactgg cgcaacgctt taccgctgct
    65701 gccctccagt ttcaggttag tcagcaccgt ttccgtggtg ctgcctttcg cttcaaaacc
    65761 gacggcgtct attactgcat ccacgccccg gtgacctgcc gtttgttcaa taattgactg
    65821 tgccggatcg ctatcttcat cgaaattaat cgggatcgcg ccgtagcggt cagcggcgaa
    65881 acgcaagcgg tagggatgat gatcaacaac aaaaatctgt tccgcaccga gcaaccgtgc
    65941 acaggcgatt gtcaacaatc ccactggacc ggcaccatag actgcaacgc ttgaaccttg
    66001 ttggatctgc gcattttttg ctgcctgcca tgccgttggc agaatatcag aaaggaaaag
    66061 ggctttatca tctgaaagca aaggcggtac tttaaacggc cccacattcc ctttagggac
    66121 gcggacatat tccgcctgtc caccaggaac gccaccatac aggtgactat aaccaaacaa
    66181 tgccgcggga gctggtatct gttttttatt gagcgcagcg cctttacccg cattggtatt
    66241 ttcgcaggcg gcatattgct gcattcgaca gaaaaaacag tcgccacaag caatgacaaa
    66301 cggaattacc acccggtcgc ctttttgcaa attttttacg tcctttccgg tttcaacgac
    66361 ttcccccata aattcatgac caaaaatatc gccatgttta acctggggta ttttgcctcg
    66421 ataaagatgg aggtcagagc cacagatcgc cgttgccgta atacgcagaa taatatcatc
    66481 tgcctgttca atgcccggat cgggaacatt ttctacctga acgtgatgtg ggccgtgata
    66541 agtcaatgct ttcatgcaac ctccagagac agttcaaaaa agagtcagtc tttaagggta
    66601 gctatcacag cgggaaaacg ggcggtaccg caggttttgg gagaattcct ggtgttcagt
    66661 gagaatatat taatcacaga aaccgttcgt aaggaaaaag aaccagacaa atatcaggga
    66721 taaatattat aatcatggcg aatgatttgt tcatgattaa cagggagaaa gggttatgta
    66781 taagacaatc attatgccag ttgatgtatt tgaaatggaa ttgagcgaca aagctgttcg
    66841 ccacgctgaa ttcctcgccc aggatgacgg agttattcat ctacttcacg tactacccgg
    66901 gtcagccagc ctgagcctgc accgttttgc cgctgatgtg cgtcgttttg aagagcatct
    66961 gcaacatgaa gcagaagaac gtctgcaaac gatggtcagc cacttcacca tcgatccttc
    67021 ccgcattaaa caacatgtcc gttttggtag cgtgcgggat gaagtcaatg agttggcaga
    67081 agaactgggg gctgatgttg tagttattgg ttctcgcaac ccatcgattt cgacccatct
    67141 gttaggttct aacgcctcga gcgtgatccg ccatgccaat ctgccggtgc tggttgtgcg
    67201 ttaattgcca gaataaatat cccgccctgc ccggagccat cccggcaggg ctttttaatg
    67261 ccataaaaaa gccgccaggt ttgaccccgg cggctaagca attgcaggtg aatcttactt
    67321 cttcttatgc agttttggtg cgaatcaggt agtcaaaagc actcagagag gctttggcac
    67381 cttcgccagt ggcgatgata atctgcttgt acggaaccgt cgtacagtca cccgctgcga
    67441 acacgccttt gacgttggtt tcgcatttcg catcaatgat aatctcaccc atgcggttac
    67501 gttcgactgc gccttccagc cagttggtgt tcggcagcag accaatctgg acgaaaatac
    67561 cggccagttc gatgttgtga atatcgccgc tgacacgatc tcgatattcc agaccaacga
    67621 ctttgctgcc gtcgcctttc acttccgtgg tttgcgcatt cagaataatg tcgacgtttt
    67681 tcaggctgcg cagtttgtcc tgcagaacct ggtcggcttt catttctggc gcaaattcga
    67741 gcagcgttac gtgctcaacg atacccgcca ggtcgattgc cgcttccacg ccggagttac
    67801 cgccgccgat aaccgctacg cgtttgcctt taaacagcgg gccgtcgcag tgcgggcagt
    67861 aggtcacgcc tttggtgcga tactgatctt cgcccggaac gttcatgttg cgccattttg
    67921 caccggtcgc cacgataatg ctgcgtgctt tcagtaccgc gccagaagct gtttcaatct
    67981 ggtgtagacc accttcaacc gctgccggga tcagtttgct ggcgctctgg ctgtcgatca
    68041 catcaacgtc gtattcatca acgtgaactt tcagtgcgcc tgccagtttc tgcccttcag
    68101 tcttcggtac agaaatgtag ttttcgatat caacggtatc gaggatctga ccaccaaaac
    68161 gttcgcccat cagaccggta cggatgcctt tacgtgcgga gtaaattgct gccgctgcac
    68221 ccgccgggcc ggaaccgacg attaatacgt cataagcatc acgcttgttc agctcttctg
    68281 ccgcacgttt ttccgcgcca gtatcaattt tggcaacgat ttcagtcaac gtcatgcggc
    68341 cctgaccaaa ctctttcccg tttacgaaca ctgccggaac gcccatcacg ttgcgatcgg
    68401 tgatttcgtt ctggaaggtg ccgccgtcaa ttgcagtgtg cttgatgcgc gggttcaata
    68461 cgctcatcag gttcagcgcc tgcaccacgt ccgggcagtt atggcaagag agcgagtaat
    68521 aggtttcgaa ttcaaaatca ccgtcaatat ggcgaatctg ctccagcaga gactgcgctt
    68581 ctttcgacgg atgaccaccg gtccacagta acgccagtac cagcgaggtg aactcgtggc
    68641 ccagcgggga gcctgcaaaa cgtggcccct ggttggaacc tgggttggtg atcaggaaag
    68701 acggcttacg caccggcaag ctgttatctt ctttaaaggt gactttgtct gacagttctg
    68761 cgatttcagc caacagttcc ttgatttctg ccgatttagc gctgtcatcc agcgtggcaa
    68821 ttaactcaac aggcttggtc aatttctcaa ggtaagcctt gagttgagtt ttcatatttg
    68881 tgtcgagcat gtttatctcc tgggctttaa acatcatcat gcaagcggcc tctgcggcta
    68941 cctggatgca gcttgcatca tggtgcgggg aagaaaatcg ggtgccgcag cacccgaaga
    69001 attaggtgaa tttccgaagt atttcgcgcc ggagttggta gcgcgaaaga cgaaggaaat
    69061 ttagatttta ccaaccaggt ccagagacgg agccagagtt gcttcacctt ctttccattt
    69121 agccgggcaa acttcacctg ggtgagaagc tacgtactgt gctgctttga ttttacgcag
    69181 caggtcagac gcgtcacggc caatgccttc agcggtaact tcgattgcct ggatgatacc
    69241 ctgcgggtca acaacgaagg tcgcacgatc agccagacct tcatcttcac gcatgttgtc
    69301 gaagttacgg gtcagggcgc cagtcgggtc gccgatcatc gcatatttga ttttagcgat
    69361 ggtttcagag ctgctgtgcc atgctttgtg ggtgaagtga gtatcggtag atactgcgta
    69421 tacgtctacg cccagtttct gcagttcttc gtagtggtca gcaacgtcac ccagttcggt
    69481 cgggcatacg aaagtaaagt cagccgggta gaagaagaag acgctccagc ggccttctgt
    69541 atctttttcg gtgatttcga tgaattcgcc gtttttgaat gcctggtttt taaaaggttt
    69601 aattttggtg ttaatcaagg acatctatac ttcctccgtg ttttcgatga gatgtaaggt
    69661 aaccaatttt tgccgattcg gctaatgcgt ttccattatc aaatcgataa gttttacctt
    69721 acaaccttcg taagacaact ttgtgaactt tgcaccgcca gcggcgataa aaagtaaaat
    69781 ggcccccttt ccaggtgacc gtgtaatctg aactaccctt tggtatcttc agcgactgcc
    69841 tgcaggctga gtgttgccct tacaaatgca acaacgacat ggattacaac accctcataa
    69901 acaaagggca atcacctgat ctaagctctt acctatgaca gtgataggtt atgcctttta
    69961 ctcgactttt gcactgactg aaaaggacaa attaatgtta aaaaagatac ttttactggc
    70021 tctgcttcct gcaatcgcct tcgcagagga acttcctgct ccagtaaaag cgattgaaaa
    70081 acagggcatt acaatcatca aaacgttcga tgcccccgga ggaatgaaag gttatctcgg
    70141 aaagtatcag gatatgggcg tcaccatcta cctgactcca gatggtaagc acgctatctc
    70201 tggttacatg tacaacgaga aaggtgaaaa cctgagtaac acacttatcg aaaaagaaat
    70261 ttacgcacca gccggacgcg aaatgtggca acggatggaa caatcccact ggctcctcga
    70321 cggtaaaaaa gatgcgccgg tcattgtcta cgtcttcgcc gatccgttct gcccatattg
    70381 taaacagttc tggcagcagg cgcgcccgtg ggtagattct ggcaaagtgc aattaagaac
    70441 attgttggtt ggggttatca agccagaaag cccggcgaca gcagcggcaa ttcttgcctc
    70501 caaagatccc gcaaaaacct ggcaacaata tgaagcctct ggtggcaagc ttaagctaaa
    70561 cgtgcctgca aacgtaagta cagagcaaat gaaagtgtta agtgacaatg agaaactgat
    70621 ggacgatctg ggggcaaatg tcacgccggc tatctattac atgagtaagg aaaatacgct
    70681 acaacaggcc gtggggttgc ccgatcagaa aacgcttaat atcattatgg ggaataaata
    70741 aaagataatt catggatggt tgaaatacat ctctgctttt caaccatcct aaaaaagcat
    70801 gaattgctcg ctctgcccta agtggctatg ctattttaga aaaacttaat cctgagttaa
    70861 gcaaaaaaat ataatcaata aaaatatata cgcaaaaaac attgattaag tgaatatacc
    70921 atggaagaaa aatataaccg gagtagtgta tggccaatct ctacgacttg aaaaagttcg
    70981 acttaaacct tcttgtcata tttgagtgta tttaccagca tttaagcatt agcaaagctg
    71041 ctgaatcact atatatcacc ccatcagccg tgagccagtc gttacaacgc ttacgtgcgc
    71101 aatttaatga ccctttattc attcgctcag gaaaaggtat cgcgccgacg acaacagggt
    71161 tgaatctgca tcaccatctt gagaaaaacc tcaaaggcct tgaacaaaca attaatatag
    71221 tcaataagtc cgaactaaag aaaaacttta ttatctacgg ccctcagctc atttcttgct
    71281 ctaataatag tatgctgatc cgctgcctac ggcaagatgc ctctgtcgag attgagtgcc
    71341 atgatatcct catgtcagca gagaatgctg aagaactgtt ggttcaccgc aaagccgatc
    71401 tggtgatcac ccaaatgcca gtgatcagcc gttccgtcat ttgcatgcca ttgcatacaa
    71461 ttcgcaatac tctaatttgt agtaataagc atccgcgaat tactgacaat agcacttatg
    71521 aacaaattat agcagaagag ttcacccaat taatatccaa atcagcagga gttgatgata
    71581 ttcaaatgga aatagacgag agatttatga atcgcaaaat ctcatttcgt ggttcctctt
    71641 tgctgacaat cattaatagc attgcagtta cagatttact gggaatagtc ccctatgaac
    71701 tttataattc ctaccgtgac tttcttaatt taaaagagat aaaactagaa catccgttac
    71761 cttcaataaa gctttacatt tcctataata aatcttcttt aaataatttg gtgttctccc
    71821 gatttattga ccgtctaaat gagagttttt gatatataac tacagacagc tactataact
    71881 ttcatctatt tattcacagc gcgaatgaaa aaataaaaca ctctgcttta cagtgtttac
    71941 tttataatta acagtcttat ctcactgttc accctgtaac gttcagagag cgtcttcaga
    72001 tgtctattta taaaattcct cttccgctca atattctgga agctgcacga gagcgtatta
    72061 cctggacact caacacccta ccccgcgttt gtgtctcatt ttctggcggc aaagactctg
    72121 gtttaatgct gcatctgaca gccgaacttg cccgacaaat gggcaaaaaa atctgcgttt
    72181 tgtttatcga ctgggaggcg caattctctt gcaccattaa ctatgttcag tccctgcgcg
    72241 agttgtacac cgatgtcatc gaagagtttt actgggttgc gctcccgctt acgacgcaaa
    72301 attccctttc acaataccaa cccgaatggc agtgctggga acctgatgtc gaatgggtgc
    72361 gtcagccccc gcaagatgcg ataaccgatc ctgacttttt ctgcttttac cagccaggca
    72421 tgaccttcga acaatttgta cgtgagtttg ccgaatggtt ttcacaaaaa cgtccggcgg
    72481 cgatgatgat cggcatccgt gcggatgagt cctacaaccg ttttgtcgcc atcgccagtt
    72541 taaataaaca acgttttgcc gacgataaac cctggactac ggccgcacca ggcggtcata
    72601 gctggtacat ttaccccatt tacgactgga aagtggctga tatctggacc tggtatgcaa
    72661 atcatcaaag tctctgcaac ccactgtata acctaatgta tcaggcaggc gttcctctgc
    72721 gtcatatgcg aatttgcgaa ccttttggcc cggagcaacg acaaggatta tggctctatc
    72781 acgttatcga accggatcgc tgggctgcta tgtgcgcacg agtcagcggg gtaaaaagtg
    72841 gcggcattta cgccggacat gacaatcatt tctatgggca tcggaaaatc ctcaagccag
    72901 aacatttaga ctggcaagaa tatgcattat tgctgctcaa tagcatgccg gaaaaaacag
    72961 ctgagcatta ccgcaataaa atcgccattt atttgcactg gtatcagaaa aaaggcatcg
    73021 aggttccaca aacccagcaa ggggacattg gcgcgaaaga tatcccctcc tggcggcgga
    73081 tatgcaaagt tttactcaat aacgattact ggtgtcgggc attatcattt agtcctacga
    73141 aagcgaagaa ctatcagcgt tataacgaac ggataaaagg aaaacgtcag gaatggggga
    73201 tactatgcaa caacgattaa cgcaggattt aacgcaattt ctcgccagct tgccggaaga
    73261 tgatcgcatc aacgcgatta atgaaatccg catggcgatc catcaggtta gcccctttcg
    73321 tgaagagcca gtggattgcg ttctgtgggt taaaaacagc caacttatgc ctaatgatta
    73381 caacccaaac aacgtggcac cacccgaaaa aaaactgctg aaaaaatcca ttgaaataga
    73441 tggttttacc caacccattg tggtcacgca tacggataaa aatgccctgg aaatcgtgga
    73501 tggttttcac cgccatgaaa ttggtaaagg ctcatcatca ttaaaattac gcttaaaagg
    73561 ttatttgccg gtgacctgtc tggaaggtac gcgtaatcaa cgcatcgccg cgactattcg
    73621 tcataatcgc gctcgtggac gccatcaaat caccgccatg tcggaaatcg tccgtgaact
    73681 tagccagttg ggatgggacg ataataaaat tggcaaagag ctgggaatgg acagcgacga
    73741 agtgctgcgc ctgaagcaaa ttaacggtct gcaagagctg tttgccgacc gtcaatattc
    73801 ccgcgcctgg actgtcaaat aactacagct ggcgcagtcg ttcagctgct gccagcaacg
    73861 tcgattcctt cttggcaaaa cagagacgaa tcagtttatg agggaaggga tcggcgcaaa
    73921 acaccgatag cggaatcgcc gctacgccgt gctcctgcgt cagccactgg caaaactcaa
    73981 catcatccag ggtagatacg gcgctgtaat ccaccagcaa aaagtatgta ccttcacacg
    74041 gtaaaatctc cagccggctt tcatttaaag cattcaccag aatatcgcgc ttctggcgat
    74101 aaaagtccgg taacgcaaga taatgctcag gttctgcacg tagcatatca gcaagcgcca
    74161 gctgtgccgg ggtattcacc gaaaaggtca gatactgatg taccttgcga atttcggcgc
    74221 tgatgggcgc tggcgcaaca caataaccca ctttccagcc ggtcatatga taggtcttgc
    74281 caaatgaaga aaccgccact gcccgctcac gcagctgcgg atgcgccagc acactggcat
    74341 ggccctgttg tgaaaagttg atgtgctcgt agacttcatc gctaatgaca aaaatctcgt
    74401 gcccggcgat cgcctgccac aaagcggcga aatcagcctg ctgccagaca gttgcactgg
    74461 ggttatgcgg agtgttgagg atcaccagtc tggtgcgctc gcttaacaat gcggcaaatt
    74521 cctgccagtc aacgcgaaaa tgcggtggtt gcagtgccat acgcttcact attcccccag
    74581 aaagcgcgat ggcgggggag taactgtcat agctgggatc aaaacaaatc acttcatcgc
    74641 cattgcgcac cagtgcggta atcgccgcgt ataacgcttc cgtcgcccct gccgttacgg
    74701 tgatatcgct atcggcatct ggttgatagc catacaaacg ttccgttttc tgagcaatcg
    74761 cctcgcgcaa ggcctgcacg ccggtcatgg gcgcgtattg gtttgccccc tgggcaacgt
    74821 ggtacgccag ccgctcctgt aaatagcgcg gaccatcaaa atcaggaaag ccttgcgaca
    74881 ggttaatcgc ctggtgttgc tgcgccagcg cgctcatctg ggtgaaaata gtggtgccaa
    74941 gttgtggaag tttgctttgt ggaatcagag ggttatttgt catttttatt gtacctgacg
    75001 aagttgcgtg ttgaaggcac tataacacga tgctagtatt tggcaatcaa gacgtttaga
    75061 tgtctaaata aaacaataag gacaacacaa catgcctcac aatcctatcc gcgtggtcgt
    75121 cggcccggct aactactttt cacatccagg aagtttcaat cacctgcacg attttttcac
    75181 tgatgaacaa ctttctcgcg cggtgtggat ctacggcaaa cgcgccattg ctgcggcgca
    75241 aaccaaactt ccgccagcgt ttggacttcc aggggcaaag catattttgt ttcgtggtca
    75301 ttgcagcgaa agcgatgtac agcaactggc ggctgagtcc ggtgacgatc gcagcgtggt
    75361 gattggcgtc ggcggcggcg cactgctcga caccgcgaaa gccctcgccc gccgtctcgg
    75421 tctgccattt gttgccgctc cgacgatcgc cgccacctgc gcagcctgga caccgctctc
    75481 cgtctggtat aacgatgccg gacaggcgct gcattatgag attttcgacg acgccaattt
    75541 tatggtgctg gtggaaccgg agattatcct caatgcaccg caacaatatc tgctggcgga
    75601 gatcggtgac acgctggcga aatggtatga agcggtggtg ctggctccgc aaccagaaac
    75661 gttgccgcta accgtgcgac tggggatcaa taatgcgcag gccattcgcg acgtattgtt
    75721 aaacagtagc gaacaggcgc tgagcgatca gcaaaatcaa cagttaacgc aatcattttg
    75781 cgatgtggtg gatgctatta ttgctggtgg tgggatggtc ggtggtctgg gcgatcgttt
    75841 tacgcgtgtg gcggcggctc atgccgtgca taacggtctg actgtcctgc cgcaaaccga
    75901 gaaatttctc cacggcacca aagtcgctta tggcattctg gtacaaagcg ccttgctggg
    75961 tcaggatgat gtgctggcgc aattaaccgg ggcgtatcag cgttttcatc tgccgaccac
    76021 gctggcggag ctggaagtgg atatcaataa tcatgcggag atcgacaaag tgattaccca
    76081 caccctgcgt ccggtggaat ccattcatta cctgccagtc acgctgacac cagatacgtt
    76141 gcgtgcagcg ttcgaaaaag tggaatcgtt taaagcctga cgtgcaaatt tagcagcagc
    76201 gcgcgccgcc ttttccaccg tagcgcgcgt cctgccgctc acggaaaaac tcttcgtagg
    76261 tcatcggcgt ttgatcggga tggttaaccc gcatatgttc gacatagttg tcgtaatcag
    76321 gcataccaat catcagcttc gccgcctgac ctaaatattt tccggctttt gccagtgaat
    76381 caaacatctt ggcctccgct aaccatgttg ctgaaagagt gccggatgcg gcgtgaacgc
    76441 cttatccggc ctacaaatgc tatcaacgcg ctcggtcagt cccctcgccc catcggggag
    76501 agggttaggg tgaggaggaa ccctcggcac tatgccaaca ttcgccaaca tcccccctca
    76561 ctctgacttt agtgtgcgcc ttttgcctgc gccacgatct cctcgacatt ttccggcatt
    76621 ggctcatacg gcgtttcttt cgccgttggc ttcggatctt tcaatgccgc cagtgccgtc
    76681 ttaatcgaga acagcgccag aaccaccacg accaccataa agaagatggt taacccggca
    76741 tccagacggt tgttgaacac cagttgcgcc agctgtgact cagtatactg cgacggaata
    76801 ttgccgctgt cgatcattgc ctggaactta ttagcaatgg ccaggaagcc gactttcgca
    76861 tccgggctaa acgctttctg ccagcctgcg gtcagggtac aaatcagcag ccaggccgtt
    76921 ggtaccagcg ccacccaggc gtaacgttga cgtttcatct tgaacaacac cacggcacag
    76981 agcatcagcg ccatccctgc cagcatctgg ttggcaatac caaacagcgg ccacagagtg
    77041 ttaataccgc ccagcggatc gaccacgccc tgatggagga agtagcccca cgccagtacg
    77101 cacagcgccg ttgccagcag gttagcaggc agtgaatcgg tccgcttcag gccaggagac
    77161 accacgccca gcagatcctg caacataaag cgcgcagcac gcgtacccgc atctaccgcc
    77221 gtcagaataa acagagcttc aaacagaatg gcgaagtgat accagaacgc cacatccatc
    77281 ataccaccca gcgcaccgtg caggatgtaa gccatcccca ccgccaacgt cggcgcaccg
    77341 cccgcacggg aaatgatcga ctgttcaccc acttcgctgg caatctggtt gagcgtatct
    77401 ggtgtaatgc taaagcccca gctactcacc acctgcgcgg cagaagcgac cacatccgcc
    77461 gtccctgccg gagccagcac cgccattggg ctgttcatgg caaagtaaac gcccggatcg
    77521 atgatacagg cggaaaccag cgccataatc gccacgaagg attccattaa cattccaccg
    77581 tagccgataa aacaagcctg cccttcgttc gccagcatct tcggcgtggt gccggaagag
    77641 atcagcgcat ggaagccaga caccgcacca caggcgatgg tgataaacag gaacgggaac
    77701 aggttaccgg tccataccgg gccagtgcca tcgacaaatt tggtcagcgc aggcatggtc
    77761 agcgtcgggc gcataatcaa aatgcctacc gccagaccga cgatagtccc gattttcagg
    77821 aaggtcgaga gatagtcacg cggggccagc agcaaccaca ccggcagcac cgccgccaca
    77881 aaaccgtagc ccaccagcat ccaggtcagc tgcacgccgg taaagtcaaa gtacggtgcc
    77941 caggtcggac tttctgccac ccagccgcca gagataatgg cgaaaatcag gaacaccagg
    78001 ccaatcactg acacttcgcc aatgcggccc ggacgcagat agcgcaggta gattcccata
    78061 aaaatcgcca gcggaatggt gaacgcaacg gtatacgttc cccacgggct atgagtcagg
    78121 gctttcacca cgatcatcgc cagtactgca aggataatga ccatgatcat aaagcaggct
    78181 accagcgcaa tcaccccggc agttggcccc atctcttctt tgaccagctc acccagcgat
    78241 cgaccgtcac ggcgcgtaga aacaaacagc accatgaaat cctgcaccgc accagcgaga
    78301 accaccccgg cgagcagcca gatcatccct ggcaggtaac ccatttgcgc cgccagtacc
    78361 ggcccaacca acggacctgc tccggcaatg gccgcaaaat ggtgaccgaa cagcactttc
    78421 ttgtccgtcg gcacatagtc cagcccgtcg ttatggcgca ccgctggcgt catacgcgtc
    78481 gggtcaaccg ccagcacatt tttggcgata tacagcccat aaaaacggta agcgatcaga
    78541 tagatacaga ccgacgccac cacaatccac agcgcgttga tctgttcccc acgatttaaa
    78601 gcaatgtatc ccagagcaaa tgctcccatt acagagagca ctgtccagac gaggtatttc
    78661 cctgatttgt tcatagttgt tatccgtgtg cgtgtccata gagatgttac attttgtttc
    78721 tataacatct ttgtacgatt taacaacacg gtaaccatgt aaatcggagt tgaaaccaat
    78781 atttaactta acaatgttaa ctcgatcact ccgttaaccg agttttcctg caatctcatt
    78841 taattctgtc gggctcatcc caaaactgcc gtacccagcc gacaagtgca gcaacgccgc
    78901 ccctgttcat cgaaaacgac gatttcccag ctctgatttt gccgcccaag atgcagcggc
    78961 tggcagacgc cgcgtacctt gccttcagac accgggcgat gatgggtggc gttaagttcc
    79021 gtacccacca cacactgccc gtcacgggtc atcataaatc cggccatcga tcccagcgtt
    79081 tcagccagcg ccgccgacgc gccgccatgc agcaggccaa acggctgatg agtacgggta
    79141 tcaaccggca tttcggcttc cagcacatca tctcccagac gggtatacac aattcccaga
    79201 tgcgccacca ttgtgttatc gctggtggcg ttcagttcgt cgagcgttaa atggcgtttc
    79261 cagatcatgc ttatgccccc agcgttgagc cgccatcgac cacaatatcc tgtagggtga
    79321 tatggctggc gaggtcagag gcgaggaaca aaatcgtgtt ggcgatctct tgtggacggg
    79381 cgattttccc cagcggaatg ccgagtttaa actgctcgcc aaagccgcga atacgctgtt
    79441 cttcggcgtc atcgctcacc cacagcgtgc gttgcatatc ggtgtcggtg gagccaggcg
    79501 aaaccacatt acagcgcacg ccgctacccg ccagttccag cccgacgctc aacgccaggc
    79561 ttttcagcgc cgctttcgat gcgccataag cactcatacc aatacgcggc gtgtgtgcgg
    79621 cgtcggacgc cacagtgaca atcgccccgc cccgctgacg gcgaaactgg ttcatggttt
    79681 gctggaacag gttaaacgca ccgccgacgt taaccgcaaa agtctgctgc cagtcctctt
    79741 tactgagctg atcggtcgcg cccatgcgca aaattcccgc cgcattgatc agcacgtcca
    79801 gtcgctccgt ttcagctaac agtcgctgac atacctgcgc cacctgcccg gcgtcggcaa
    79861 catccattac ttcggtcgca aagggatatt gctcctgagt gaacgcttga tcaaaacctg
    79921 taactttcgc tcccgcctca acaaacgcca gcgccgtggc gtagccgata cctttacctg
    79981 cgccggttac ccagacattt ttaccgctga aatccattat ttcacctcgc gggagagtag
    80041 cttccaccag gcgtcgatgg tcgggttttt cgccagcatg acaaagtcga tatcaccatg
    80101 cactttgcgc cagcgcgccg ccagcgccat catgcgcacc gaatccagac cgtagtcgat
    80161 caggttgtcg tcatcgaacg gttcatcgga ctcgtccagc aacggcagga taacctcacg
    80221 cagcgccgct ttgctggcgg ggacaggtgc tggcagtaat tcttcagtca tcaccacccg
    80281 gccagaccgc ccggccacat atttcagcga catcaaatgc tcgtcacggc tgaaatcggc
    80341 cagcgcatcc gccaccataa acggtttaat atcgcgcata aatgcgtcgg ttgcggtggt
    80401 catacagcca atgtgggcat ataccccggt aataatcagc tggttacgtc cactctcttt
    80461 gagcatttgc tccagcggag aacgatgaaa cgcgctgtag cgccacttca ccagcaccgt
    80521 gtcgtcggca tctggcgtca ggcgatccac caccttttgc tgttccggcg aacgggtcag
    80581 gcccggcccc cacatatcat tcaacagcgc ccgatcttca tcgctctgct ctttcggctg
    80641 ggcggtgtaa taaaccggga tattgtgctg tttgcagtag tcgcgcagcg cagcaatatt
    80701 cgcgatcacc tgctccatca tcgggcagtt ctcgccccag aagctgacaa aatagtcctg
    80761 catatcatgg attaacaacg cggcacgttg cggttcaaag gcccagtcaa ctttattctg
    80821 cggaatatcg tgagactccg gcagtgcgta agcctgtaat tttggaatag ccatagtgtt
    80881 ctctccttca ggctgatgcg cgtgacgcca gccactgacg taattgtttt ttatcgactt
    80941 tcccgaccgc cgtcagcgga agtgaatcca cacactccac gcgatccggt aatttaaatt
    81001 cggcaatacc ctgttcacgc aggaaacgac gcacctgcac cgcgcgcagc ggctctttta
    81061 ccaccagata agcgcagctt ttttcgccca tcagctcatc ttccatgctc accagtgcgg
    81121 catagatcac tgccggatgg cgcagcagca ggttttcgat ctcttcggca gcgatcttct
    81181 cgccgccacg gttgatctga tctttctcgc gcccctgcac ggtgatgtaa ccctctggat
    81241 caatagagat cagatcgccg gaacagtaaa aaccgttggc atcaaaggcg ctggcattgt
    81301 gctgcggact tttgtaataa ccgcggaagg tgtacggccc gcgcgtcatc aggcgtccga
    81361 cttccccttg cggcagtgga tttccttcgg catcggcaac ccacacttca tcgtccggac
    81421 acattgggta accctgggta tggataattt tctccgcgct atcatcaaga cgggtgtagt
    81481 tcaccagccc ttccgccatg ccaaacacct gctgcaactg acagccaatc tcagcgggaa
    81541 tacgcgccgc aagggtggca gaaagacgtg cgccgccgac ctgtaacagt ttcagcgagg
    81601 caagctgcgc ccggctttcg ccttcggtca gcgcctgcaa ccacaggctg actgcgggtg
    81661 gcaccagcgc ggtaacgtta acctgatgtt tttcaatcaa tgggaaacag agcgtggcgc
    81721 tgggatcggc cgccagaaca accgttcctc cggcaagaaa gacgcccagc gatcccggcg
    81781 aactcatggc gtagttatga gccgccggga tcgcgcacag gtagcgtgtc tgttgtgtga
    81841 actgacaaat ctcgacgcta cgacgcacgc tgtagtagta gtcgttatga gtgcgcggga
    81901 tcagtttcgg tgtgccggtg gtgccgccgg aaagctggaa ataggccact tcatcagcag
    81961 gtgatggcgt ggcagtaaaa tcctcagccg gatggttaat cgcatcctgc aagttatgct
    82021 caccgctgtc gttgtgcagt tgcaccacgc gaatggagga atgttctgtg acgaaagtat
    82081 tgaggaaatc atccccgcta aacagcgcat gttggcgatc ggcaatcagc aatgcgggtt
    82141 caatctggct ggcataggcg ttcagttcac tacgctgatg gctgaacagc gccagcaccg
    82201 gcgcaacgcc cagtttcagc agcgcgaaaa aggtaatata caattcagcg acgttaccca
    82261 gttgtaccag cgcggtttca ccaggtttaa tgccctgacg gcgtaaacta cacgcgaggt
    82321 tatccgccgc ctgattcagc tcccggtaac tcaactgtcg ctcgccgtcg ataaccgcga
    82381 tgctgtcact cgcagcgtgg cgagtcagaa tgtcggtcag cggcaaatcc tgccagtagc
    82441 ctttttcccg atagcgacgg gcaaactctt ccggccagcg ggtgaatgga atgctcatcc
    82501 tcgctcctta atgcaatcca aaaacgttca acatggtaga aagtttgacg cctgtttcgc
    82561 gccactcacc caacggtgac gacgcaggca caatccccgc tccggcaaac agacgcacct
    82621 gattttcccg cagcttcgcg cagcggatgg tcaccaccca ttcgccgtta ccttcgctgt
    82681 cacaccaacc cacaatgccg ccaaacagtt cgcggtcgaa tggctccagt tcagcaataa
    82741 cctgggtcgc ggcctgatgc gggaaaccgc tcagcgcggg ggttggatgc agcagacagg
    82801 ccagagtcag tgcgttttct tgcgaattcg ctttaccttc aaagggagtt gcgagatgcc
    82861 acagcgtcgg cgtggtaatc aattgtggag aggagggaac gtgtaactca ctactgcgtt
    82921 cgcgcagtac ctctttcatc gcctgagtca ccagttcatg ttcatggcga tctttttctg
    82981 acgccagcag acgattaccc gcttcgcgat cgagcacttc atccggctga cgacgcgcgg
    83041 aaccggctaa cggaatggag ctaaaacgct cgccgtcttt acgtagcagc agttccgggc
    83101 tggcccccag caggacgcca ccatcagcca gcgggacatg gaagttgtaa ctaaccgggt
    83161 tttgcgcaat caaccgttcc agcaatacgc cactatcaat ggcggcgtca gtggtgatat
    83221 caatcaatcg tgacaacacc actttgtcga cctgcggcgt ggcggtaagt gcggcggcgc
    83281 gagcaaccat ctgttcaaac gtggtttgtt caggaattgc ctggcgttcc accacgttca
    83341 gcgactggct gcgggtaaaa cggcgggctg aggtctgttt ttcctggcgg gagaacgact
    83401 gccaggattc gggaatatac agcgacgaag gctgacgtgg atcgaaggga atcgccccga
    83461 ccatcaccgg atttttgatg ccctgcgctt tggcatcggc aaacagcgcg gcgagttttt
    83521 gctggaaggg actgtcgggc gaatccccgt tcacagccgg ttcatcgaag cgggcgaaac
    83581 atcctgacgt cgtaaaactg cggtacggcg acataaagaa aaagcgattg ggcgcaagtg
    83641 ttgccatggt ctgctgtact tcctcagcca gtgacgtatc catatcatcc tccacaaaat
    83701 gataaaggct ttaataatga ttatcattta tattttcggt cgctaaccta aaagcaaacg
    83761 cgcactatgt caactcttga ggtaacgcaa tctgcaactc ggggttgcat ctggttgtgg
    83821 ttacaatgaa aatgagaagc attattgatg gattcgcata agcgcaatgt gatgtcctgc
    83881 gccgttctgc cccctctccc ttccagggtg agggctgggg tgagggttaa tgttcgcacc
    83941 agtgctggct gttcccctca ccctaaccct ctccccaaag gggcgagggg acggattgtg
    84001 cgctttgtcg aatttgtcat tacgccctta accttattaa taacaggaag ctgatttgtg
    84061 agactcgccc cgctctaccg caacgccctt ctattaacag gacttttgct ttcaggaata
    84121 gccgcagttc aggccgctga ctggccgcgt cagattactg acagccgtgg cacacataca
    84181 ctggaaagcc agccgcagcg tattgtttcc accagcgtca ccctgaccgg ctcactgctg
    84241 gcgattgatg ctccggtgat cgccagcggc gcgaccacgc cgaataaccg cgtcgcggat
    84301 gaccagggct ttttacgcca gtggagcaag gtggcgaaag aacgcaaact gcaacggctc
    84361 tatatcggcg aaccgagcgc cgaagccgtt gccgcgcaaa tgccggatct gattttaatt
    84421 agcgcaaccg gcggggattc ggcgctggca ctgtatgatc agctttccac catcgccccg
    84481 acattaatca tcaattacga cgacaaaagc tggcagtcgc tgttaacgca gcttggcgaa
    84541 attaccgggc atgagaaaca agcggcagag cggattgcac agtttgataa gcaactggca
    84601 gcggcgaaag agcaaatcaa attaccgccg cagccggtca ctgccattgt ctataccgcc
    84661 gctgcacaca gtgccaatct ctggacgccg gaatcagcac aagggcagat gctggaacaa
    84721 ctcggcttta cgctggcgaa gttgcccgca gggttaaacg ccagccaaag ccagggcaaa
    84781 cgccatgaca tcattcagct tggtggggaa aatctggctg ctgggttaaa tggcgagtca
    84841 ctattcctgt tcgccggtga tcagaaagat gccgatgcga tttatgccaa tccactgctc
    84901 gcgcaccttc ccgcagtaca aaacaagcag gtttatgcgc tgggaaccga gacgttccgt
    84961 ctggattact acagcgccac gcaagtgctg gaacgactta aggcgctgtt ttaagcatta
    85021 actgtcagac gctgtcacct gcggcggcgt ctggcgaaaa cgtcgcaact ccaccagcac
    85081 cagcaataac aacacgccga taatcaacaa accaaaaccg cttgcgcttg cggaagcaac
    85141 cggtgtcatg atcgcgccca aaccacccag cagcgccgcg cctatggcat cgcccgtcac
    85201 gttctgcgcc gtccacaaac cgttaatccg ccctaacatc gcttccgggg tttgcgtttg
    85261 cagcattgta tattgcagca acgaactaac cgcgctcagc cagccgaaca gcgccagaca
    85321 aaccacgcct aaaatccaca tcggcatcag gccaaacaga ccaatggcga ggaacgatcc
    85381 cagcgtggag agcagcatca ataaccctgg tcgcgcacta tgtgccagct tcccgctggt
    85441 taacgcacca atagccgcgc cgagcgggat cgccgcgtag agaaaaccaa tctgtgccgc
    85501 tgacatctgc cagttgtcag ccagcgccgg atacagtacc cgcaccgcgc tcgccatcgt
    85561 caataaacca cccagcagcg caatcccgcc caccagcggg ctggcaagca gaaaacgaaa
    85621 tcctgccagt aatgatttca acggatgctc acgcggctgc ggtggcggtg gcaacgccgg
    85681 aaggcttaac aacggtagca aggtaataaa cgtgcccgcc gccgccagcc cgtagttcca
    85741 ggctacgcca ccggtcgcca gcaataaacc gccaatcatg ggcgaaatca ccgaccccag
    85801 acgcacggtc aacatggtga tcgccccggc ctgcattaag ttttcacgcc ctaccagtgc
    85861 tggtgtcgcc gccaatagcg ccgtaacgcc aagcgatgcg aaaaaaccat cccataaacc
    85921 aagtaaatag attgccagca atgacggctc cggcagcagt gcattaaggc acagtccaat
    85981 gaagccaatg ccacaggtgc cgcgcgccag caaaatcact tttttgcgct catagcgatc
    86041 cgccagcaca ccgccaacca tcaggccaac aaacatcgcg ccgccggtca gcgtcaccga
    86101 aagccccacc tgccaggtag aatgcgtcat catctggatc tgcaccggca ccgcgacgcc
    86161 gagcaaaccc agagacacaa ttgagataaa acgagcgagg aatactgcgc gaaacgccgg
    86221 gtgcgttttc aacaggctga ggttaagcag ccaggattgt ttattcatta caatgccttg
    86281 ccatcaaata ttttaaatac ctattcttag gctgcacatg ctaacatatc caaataagat
    86341 cgataacgat aattaatttc attatcatgg aagttcgtat gtctggttct gttgccgtga
    86401 cacgcgccat tgccgtgccc ggattgctgt tattactgat tatcgcgacg gcattaagcc
    86461 tgctcattgg ggcaaaatca ctccccgctt ccgtagtgct ggaggccctc tccggcacct
    86521 gccagagcgc cgactgcacc atcgtgctcg acgcgcgact gccgcgcacc cttgccggtt
    86581 tacttgcagg cggcgcgctt ggccttgccg gggcgttgat gcaaaccctc acccgaaatc
    86641 cacttgccga ccccggcttg cttggcgtga atgccggagc cagctttgcc attgtgctgg
    86701 gtgcggcgct gtttggttac tcatccgcgc aggaacaact ggcgatggcc ttcgccgggg
    86761 cgctggtggc ctcattgatt gttgccttta ccggcagtca gggcggcggg cagttaagtc
    86821 cggtgcgttt aaccctggcg ggcgtggcgc tggcggcggt gctggaaggg ctgaccagcg
    86881 gcatcgccct gcttaatcct gatgtctacg atcaactgcg tttctggcaa gccggttcgc
    86941 tggatattcg caatctacat accttaaaag tggtgctgat cccggtgctg atcgccggag
    87001 caaccgcgct attactgagc cgcgcgctga acagtttaag cctcggcagc gacaccgcga
    87061 cggcgctggg cagtcgcgtg gcgcgcacac agttaattgg tctgctggcg attaccgtgc
    87121 tttgtggtag tgcgacggcg gtagttggcc cgattgcctt tatcggcctg atgatgccgc
    87181 acatggcgcg ctggctggta ggtgccgatc atcgctggtc gctgcccgtc acgctacttg
    87241 ctacccctgc cctgctgctg tttgccgata tcatcgggcg ggtgattgtg cccggcgaac
    87301 tgcgcgtttc tgtggtcagt gcgtttattg gtgcaccggt gctgatcttc ctcgtgcgac
    87361 gtaaaacgcg aggtggcgca tgatttacgt ctctcgccga ttaatcatca cctgtttgct
    87421 gttggtttcc gcctgtgtgg ttgcaggtat ctggggatta cgcagcggtg ccgtcacgct
    87481 ggaaacctcg caggtattcg ccgcgctgat gggcgatgcg ccgcgcagta tgacgatggt
    87541 ggtcaccgaa tggcgtttac cacgcgtgct gatggcgctg ttgattggcg cggcactggg
    87601 cgtcagcggc gcgatttttc agtcgctgat gcgtaacccg ctcggcagcc ctgacgtaat
    87661 gggctttaac accggggcgt ggagcggcgt gctggtagcg atggtgctgt ttggtcagga
    87721 cctgacggct atcgcgctgg cagcaatggt gggcggcatt atcacttcgc tgttggtctg
    87781 gctgctcgcc tggcgtaacg gcatcgacac ctttcggttg attattatcg gtatcggcgt
    87841 tcgtgccatg ctggtggcct ttaatacctg gctgttgctg aaagcgtctt tagaaacggc
    87901 gctaacagcg ggtttgtgga atgcaggatc gctcaacggc ctgacgtggg caaaaacctc
    87961 gccttccgca cccatcatta tattgatgct cattgccgcc gccttactgg tacgccggat
    88021 gcgcttgctg gaaatgggcg atgataccgc gtgtgcgctg ggcgtcagcg tcgaacgttc
    88081 gcgtctgtta atgatgctgg ttgcagtggt gcttaccgct gcggcaacag cgcttgccgg
    88141 gccgatttcc tttattgctt tagtcgcacc gcacattgcc cgacgcatta gcggcaccgc
    88201 tcgctggggg ctaacccagg cggcgctatg cggggcgctg ttactgctgg cggccgatct
    88261 ctgcgcccaa caactgttta tgccgtatca acttccggtt ggcgtcgtta ccgtcagcct
    88321 cggcggtatt taccttatcg tcttgttaat tcaggagtct cgcaaaaaat gaccgaatca
    88381 gtagcccgtt tgcgcggcga acagttaacc ctgggatatg gcaaatatac cgttgcggaa
    88441 aatctgactg tagaaatacc tgatggtcac ttcacggcaa ttatcgggcc aaatggctgc
    88501 ggtaaatcca cgttactgcg taccttaagc cgcctgatga cgcctgctca tgggcatgtc
    88561 tggctggatg gcgagcacat tcaacattac gccagtaaag aggttgcacg ccggattggt
    88621 ctgttggcgc aaaatgctac cacgccgggc gatatcaccg tgcaggagct ggtggcgcgt
    88681 ggacgttatc cgcatcaacc gctgtttacc cgctggcgca aagaggatga agaagcggta
    88741 acgaaagcaa tgcaggccac gggaataact catctggcag atcaaagcgt ggataccctt
    88801 tctggcggac aacgccagcg agcgtggatc gcgatggtgc tggcccagga aacggcaatt
    88861 atgctgctcg acgaacccac gacctggctg gatatcagtc atcagattga tttgctggag
    88921 ttgttaagcg aactgaaccg cgaaaaaggc tataccctgg cggcggtgct gcacgatctt
    88981 aatcaggcct gtcgttacgc cagccatttg attgcattgc gggaagggaa aattgttgct
    89041 cagggagcgc cgaaggagat tgtcactgct gaactgattg agcgcattta tggtctgcgc
    89101 tgcatgatca ttgacgatcc agttgccgga acgccgcttg tggtgccgct cggacgaacg
    89161 gcaccttcaa ccgcaaatag ttaaactaag tggtctgcca tcatggcatc ctgttttctg
    89221 gatgccatcg catagcgcaa cagcacgcca ccacaagcca ccatcccgcc gatcaacgcg
    89281 gaaaggatca caataatcgc cttacccgga ccgtcttttt tcactggcaa tgacgggctt
    89341 aactgatatt taaacggcgt aaaattcaca tcgttgacat gtgcttttgt taattgctcg
    89401 acaagatact gccgattacg taattcaccg ttcagttccg caacgtcagt gaccgctttt
    89461 tctatttcca gtttgcgttc aataccgtct gcaccgagag aaatagaaaa atcggggtca
    89521 tctttaacgg cctgaccatt actgtaaacg ggctttttaa ttcctgccgc gttggcaatg
    89581 tcgagtgaat aattgaggcg ctgaatgttt gcatcaagtt gatttttcgt tttaatgcga
    89641 tcctgagcca gtttttcttt ttcaaactgg gttttgatct ccagtttatt acggacgttt
    89701 tctagcgact ctttcaccac caacgtagag atataatcga tatacccgct caaaacggtc
    89761 tgcgcctctt cactggttgg cgcggtaaaa cttagcgtcc aggaggtata cagtgacggt
    89821 tcatcttttt tcttactggc attgtcatca accgctttca ttttttcgct caatgcgaca
    89881 attgcgcgat gcaaatccag ttcgtcgatt ttcgcctctt ttaattggtc catcacataa
    89941 ggtgatgaac gcaggtactc ttccagcaag ctaaccgact gaaacttctt gataaacagg
    90001 ttaaatgctt ctgtacgatc aattttgata tccagatcca gcactcgaag cttagtgaat
    90061 gttttctcca gctcttgcca ctgaacaggt tctggaggcg tgacaaccgc cgcgctggtc
    90121 catttttgcg gcaggatgaa agagatcagc aagcctgcgc aggcaaacgc aaaaatgacc
    90181 gccatgaccg tttttttggc ccgccataaa actgcgatta gattaagtaa atcaatttca
    90241 ttattactgg gcgacgccag aggatactca ggaaaatgag cgtcacttcc ctgtttaata
    90301 ttcagtgatg acatggggta taacctgaaa atgagtccaa taggggaaat ctccacggcg
    90361 ctcactttac cagttggtga taattatccg aagctgaagt ttgtaaattc cttccactga
    90421 atgcaacatt cactctattt aagagaaata ttcatcaaac ctataaatca aacaaataaa
    90481 aaaacccata attattttct gtaattatgg gtttatagat attattaatt tacctgttta
    90541 gcgttgcgcg aataatcggc ccaattttta caaacgcccc tggagagata atatccacat
    90601 gcgcacaatc ctgacgatag atatccagct ccgcaatcca cggcgaccag gcacgttcgg
    90661 gactcatacc ttcctgaagt gtgcgctcag caacaaataa cgttgctttt ccatcaaacg
    90721 gaacgctatg ggcggtggtt aacaagcgca cagcatcagc gtagttgcct tcaatggtgg
    90781 taaacaactc cgttgaagta cttccctgct gtgccgccag gaaggcctcg cgttcgcggt
    90841 taatctccgc cagcacttcc gggtccagac ctttagcctc tttttcctgc cagttttgcg
    90901 tctctggcgg ccaggtatcc agcaaaccaa gaaatgccac ctgttcgcca cgggcacgca
    90961 gtcgcgccgc aataccctgc gccagcgtac cgccaaggga ataccccagc aggtaataag
    91021 ggccgtgcgg ttgttgctca agtaacgttg ccagatgcgc ttcgcagact tcatccaggt
    91081 ttgtcgctgt ctgcatgggt ccatgagggc gcggcgactg aatgccaata atcgaccatt
    91141 gtggatcgag ataacgcgag agcacgctaa actgccaggc aaaaccggac gccggatgga
    91201 aacaaaacag cgtcgggcca ttaccttcac gcaacggcag aatggtttcg aatcccatgc
    91261 gccgggagct gtcctcttca ccatcaataa tcgttgccag tttggcgacg gttgacgcga
    91321 ccatcacctg ccccggcgtc acctgacgag caaactgtcg acttaactgc gctgctagtt
    91381 tcattgccag tagcgaatga ccgcccagcg caaagaaatc agcatcggca tcctgcacgt
    91441 cacaacccag caacgacgcg aatgccgcgg cgataatcgt ttcactgccc gctttcggtg
    91501 cacgtcccga cgcttgtgtc ttcagttcag gcaacggtaa ggctttgcga tccagcttgc
    91561 cgttggcgct aagcggcaac tgcggaagtt gcagcagaac gaccggcacc atatgcggcg
    91621 gcaatgtttc gcgaagctgt gcctgtaatg cgctggtatc caacggcagt cccgattgcg
    91681 acaccagata gcccaccaac tgacgcgcat caccaccggt ggctgccgcc tggttaatca
    91741 cacaggcgtg ggtaactgcc tgttcgacat ccggcagcgc ctgcatcacg cgatcgattt
    91801 cgcccagttc gatacgctgc ccgcgaattt ttagctgatc gtcactgcgc ccgaggtact
    91861 ccaccgcgcc gttatccagc cagcgggcga cgtctccggt acgatacatc cgttcacctg
    91921 gggcaaaagg atcggcaata aaacggctgg cggtcagatc gggtcgtcca agatacccct
    91981 gcgccagttg gatgccggtg agatagagat cacccgccac acccggcggc accggatgca
    92041 tcatcgcatc aagaatacgc agacccgtat tccataccgg ataaccaatc ggcacactgc
    92101 tgccgcgcac ctgtgccagt tcctcgccaa aagccggata ccagctgaca tctaccgccg
    92161 cttccgtcgg gccatacagg ttatgcaacg gcgcgccggt taactgttgc cattcgcggc
    92221 ataaatcagc cggtaaggcc tcaccactac agaaaacctg tttcaacgtc gcgcaattct
    92281 ggcgagcggt ttgcggcgtc agcgatgcaa caaatgccgc cagcatcgac ggcacaaagt
    92341 gcgtggtcgt tacgccatat tcggcaaaga attgctgcat agcgagcggg tcacggtgcg
    92401 cttccggttc agccatcacc agttttgccc ctgcgataaa cggccagaaa aactcccaca
    92461 ccgagacatc aaaactgcac ggcgtttttt gggcaacgac atcttcgcct gtaagcgggt
    92521 agtgattttg catccataac aggcggttaa cgatagccgt ctgcccgacc attacccctt
    92581 tcggcctgcc ggtggagcca gaggtaaaga tgatataagc cgtgtggtgc ggttgtgaaa
    92641 gttgcagcgg cgcactgccc tggggggtaa gcggggcgtt atagcaaagg cttgttaaat
    92701 tgggaacatc gctaaagcgc ggcagttgat cgtcggtggt aattaacagc gacggacgcg
    92761 catcttccag catcattttc aggcgatcgt ccggatagcc ggtatccagc ggtagccagg
    92821 ccgcgccagc ttcaactatt gcatggagtg ccagggtcaa aaagaccgag cgcggtagtg
    92881 ccaccgccac gctgtcccct ggtttaacgc cgcgctcacg cagcagattc gccagcgcca
    92941 ccacctgctc gcgcatttcc cgatagctga acaggtaacg cgcatctgcc agcgccggag
    93001 catccggtgt ttttgccgct tgttctgcca ccagcgcgct cagcgtggtt tctggaatct
    93061 caacctgagt ggcgttgatc tgcgccagct gcgcatactc acctggcagc ataatatcga
    93121 catcgccgca caacagcgcc ggatccgcgg cgaactgggc aatcagcatt ttcaggcgtt
    93181 cagcatgctg gattaacgtt ggctcatcgt aacgctgttt attggcgagg atctcaatac
    93241 tcaaatcacc gtgtacatcc gggaacaggg ccagttcaag gtcattaacc ggaccggttg
    93301 ccagggtatg ggtttgcgcc tgaacatcag gaatatccag ttggtaatca aataccttga
    93361 tattgagtac cggaccaaac agcggttcat cacctgccgc tcgcccgctg tcacggacaa
    93421 tttgttcggc atcgtaacgt tgatgacgac gcattttttt cagttgtgct gccagtcggg
    93481 ttgccagttc cggcagcgtt tcttgcgccg caatgtgaat acccaacggc aaaacgttga
    93541 gcacgggtcc ggtagccgtc agcgccgccg agcccagtcg acgcataaag ataaatccgg
    93601 cggcgtagtc catacgattg cacaatcgcc ccagccacaa ggctgccagc gcaagggcta
    93661 aatcggtacg ctgcacacct gaaagttgcg tagccagctg gcggaattcc ccgtcggtaa
    93721 attccagttt caggcgcaga atatctgccg aggcgctgcg ccccggtaaa ggtgccggag
    93781 aaagtgacgc gggcggcggc agttgacgac gctgttctgc ccagaatgcc gcatcacgct
    93841 gccaggcttc gctttcgcgg tattgctggt actcttccac tacatcagcg aaaggcgtaa
    93901 atggcgaagc aggcgttggt tcgccacgca gccatgtgca gtaaatattg gcgatctggc
    93961 gggtaatggc cgggaaactg aagccatcga ccagcaaatg gtgataacgc tgataccagt
    94021 accagcggtt atccgccacc tgtatcagct gatgaaagac cagtggttta ccgctatcga
    94081 cgcgcagatc ttgttgcaaa tccgcctgca ttaatgcctg cgcagtaccg tgcggatcaa
    94141 tgttggttcg taggtcgata atttctggca gttcgaacgt cagcgcatca tcgacccact
    94201 gccagacttc gccgttatct tccgtaaaac gcatccgcag cgtatctgct tgcgctagtc
    94261 cggcaaccac cgcgcgggcc agtaatggcg aatcaacctc tccggttaac tcaacgtaat
    94321 gcgccacgct ccaggcggag ggtaattctg acagtttttc tgccatccag atgccgggct
    94381 gtgcggcgac caaaggtaaa tgctggctca ttgtgcctcc tgcaactggg taaaattcgt
    94441 cggtgtcaga gtacgccagt gggcttccag ccactgctgg caggacgcct gtgactgcgg
    94501 ctgacacaca atgtcccagc ctgccggtaa ggcgcattgt tgcggccaca gactgaattg
    94561 cccctgcgca ttgcgcaaaa tgtaaaacgc tccctgcgga tcatcgaagg gattactgaa
    94621 tgccatattc aactcctgtc atggaaaagt ggttgccaga ggtcgattag cccctgcatc
    94681 aagccaccgc gccaacaaag cgcatcatgt ccgccgtcaa cctgacgcca gaaaatggat
    94741 tcttttatgg ggtgtaattg cgcatacagc gcctgattgg cccgcataat catcggctcg
    94801 cgaatacccg cttccagcac aatgcgcaga ccttcggcgc taacttcacc agctttaagc
    94861 ttttcaagta acacgccctc ttgctgcccg ccccgatgcg gccaccagta cgatcctgac
    94921 tggcttaata cacagccaaa gcgttcaggc cagtgcagtc cggcatacag cgcggacagc
    94981 ccaccaaaac tctgcccggc aaccacggtg cgatcgccgc gatcgctaaa aggggcaata
    95041 gctttcacca ggggtaataa ctcttgctgt actgccagcc agaaatccgc attacacggc
    95101 agttcgtggg cgcggtgcgt ggtgtcgata gcgtc
//
LOCUS       DAMZET010000003        86407 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.003, whole genome shotgun sequence.
ACCESSION   DAMZET010000003 DAMZET010000000
VERSION     DAMZET010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 86407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.003"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(136..681)
                     /gene="hemG"
                     /locus_tag="POB34_000227"
     CDS             complement(136..681)
                     /gene="hemG"
                     /locus_tag="POB34_000227"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HDI5917245.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
     gene            complement(693..2144)
                     /gene="trkH"
                     /locus_tag="POB34_000228"
     CDS             complement(693..2144)
                     /gene="trkH"
                     /locus_tag="POB34_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HDI5917246.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2183..2797)
                     /locus_tag="POB34_000229"
     CDS             complement(2183..2797)
                     /locus_tag="POB34_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HDI5917247.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2797..4128)
                     /gene="pepQ"
                     /locus_tag="POB34_000230"
     CDS             complement(2797..4128)
                     /gene="pepQ"
                     /locus_tag="POB34_000230"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HDI5917248.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4318..6507
                     /gene="fadB"
                     /locus_tag="POB34_000231"
     CDS             4318..6507
                     /gene="fadB"
                     /locus_tag="POB34_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HDI5917249.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLQFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6517..7680
                     /gene="fadA"
                     /locus_tag="POB34_000232"
     CDS             6517..7680
                     /gene="fadA"
                     /locus_tag="POB34_000232"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HDI5917250.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(7864..8565)
                     /gene="fre"
                     /locus_tag="POB34_000233"
     CDS             complement(7864..8565)
                     /gene="fre"
                     /locus_tag="POB34_000233"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HDI5917251.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8611..10104)
                     /gene="ubiD"
                     /locus_tag="POB34_000234"
     CDS             complement(8611..10104)
                     /gene="ubiD"
                     /locus_tag="POB34_000234"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HDI5917252.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10271..10759
                     /gene="rfaH"
                     /locus_tag="POB34_000235"
     CDS             10271..10759
                     /gene="rfaH"
                     /locus_tag="POB34_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HDI5917253.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10756..11538)
                     /gene="tatD"
                     /locus_tag="POB34_000236"
     CDS             complement(10756..11538)
                     /gene="tatD"
                     /locus_tag="POB34_000236"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HDI5917254.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(11580..12356)
                     /gene="tatC"
                     /locus_tag="POB34_000237"
     CDS             complement(11580..12356)
                     /gene="tatC"
                     /locus_tag="POB34_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HDI5917255.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12359..12874)
                     /gene="tatB"
                     /locus_tag="POB34_000238"
     CDS             complement(12359..12874)
                     /gene="tatB"
                     /locus_tag="POB34_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HDI5917256.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(12878..13147)
                     /gene="tatA"
                     /locus_tag="POB34_000239"
     CDS             complement(12878..13147)
                     /gene="tatA"
                     /locus_tag="POB34_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HDI5917257.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13226..14866)
                     /gene="ubiB"
                     /locus_tag="POB34_000240"
     CDS             complement(13226..14866)
                     /gene="ubiB"
                     /locus_tag="POB34_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HDI5917258.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(14863..15468)
                     /gene="ubiJ"
                     /locus_tag="POB34_000241"
     CDS             complement(14863..15468)
                     /gene="ubiJ"
                     /locus_tag="POB34_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HDI5917259.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15482..16237)
                     /gene="ubiE"
                     /locus_tag="POB34_000242"
     CDS             complement(15482..16237)
                     /gene="ubiE"
                     /locus_tag="POB34_000242"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HDI5917260.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16332..17759)
                     /gene="rmuC"
                     /locus_tag="POB34_000243"
     CDS             complement(16332..17759)
                     /gene="rmuC"
                     /locus_tag="POB34_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HDI5917261.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQYAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(17900..18661)
                     /gene="udp"
                     /locus_tag="POB34_000244"
     CDS             complement(17900..18661)
                     /gene="udp"
                     /locus_tag="POB34_000244"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HDI5917262.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            18923..19738
                     /locus_tag="POB34_000245"
     CDS             18923..19738
                     /locus_tag="POB34_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDI5917263.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            complement(19777..22038)
                     /gene="metE"
                     /locus_tag="POB34_000246"
     CDS             complement(19777..22038)
                     /gene="metE"
                     /locus_tag="POB34_000246"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDI5917264.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            22156..23109
                     /gene="metR"
                     /locus_tag="POB34_000247"
     CDS             22156..23109
                     /gene="metR"
                     /locus_tag="POB34_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HDI5917265.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            complement(22997..23896)
                     /gene="bioP"
                     /locus_tag="POB34_000248"
     CDS             complement(22997..23896)
                     /gene="bioP"
                     /locus_tag="POB34_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HDI5917266.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(23972..24772)
                     /gene="yigL"
                     /locus_tag="POB34_000249"
     CDS             complement(23972..24772)
                     /gene="yigL"
                     /locus_tag="POB34_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HDI5917267.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTAHGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            complement(24780..25802)
                     /gene="pldB"
                     /locus_tag="POB34_000250"
     CDS             complement(24780..25802)
                     /gene="pldB"
                     /locus_tag="POB34_000250"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HDI5917268.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            25913..26533
                     /gene="rhtB"
                     /locus_tag="POB34_000251"
     CDS             25913..26533
                     /gene="rhtB"
                     /locus_tag="POB34_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HDI5917269.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            complement(26595..27215)
                     /gene="rhtC"
                     /locus_tag="POB34_000252"
     CDS             complement(26595..27215)
                     /gene="rhtC"
                     /locus_tag="POB34_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HDI5917270.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQIELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(27279..29108)
                     /gene="recQ"
                     /locus_tag="POB34_000253"
     CDS             complement(27279..29108)
                     /gene="recQ"
                     /locus_tag="POB34_000253"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDI5917271.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            complement(29241..30110)
                     /gene="pldA"
                     /locus_tag="POB34_000254"
     CDS             complement(29241..30110)
                     /gene="pldA"
                     /locus_tag="POB34_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HDI5917272.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            30275..30742
                     /locus_tag="POB34_000255"
     CDS             30275..30742
                     /locus_tag="POB34_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HDI5917273.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            30794..31684
                     /gene="rarD"
                     /locus_tag="POB34_000256"
     CDS             30794..31684
                     /gene="rarD"
                     /locus_tag="POB34_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDI5917274.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            31743..32159
                     /locus_tag="POB34_000257"
     CDS             31743..32159
                     /locus_tag="POB34_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917275.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRRRYIFSFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIVLHYLVINMNCQRLRDSGFIYIKTYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            32173..32553
                     /locus_tag="POB34_000258"
     CDS             32173..32553
                     /locus_tag="POB34_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HDI5917276.1"
                     /translation="MSKEYMNDGSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(32596..33546)
                     /gene="corA"
                     /locus_tag="POB34_000259"
     CDS             complement(32596..33546)
                     /gene="corA"
                     /locus_tag="POB34_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDI5917277.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            33916..34680
                     /locus_tag="POB34_000260"
     CDS             33916..34680
                     /locus_tag="POB34_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HDI5917278.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLI
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKEIQFAVQSGP
                     MLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFSCYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(34827..36989)
                     /gene="uvrD"
                     /locus_tag="POB34_000261"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(34827..36989)
                     /gene="uvrD"
                     /locus_tag="POB34_000261"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HDI5917279.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(37073..37789)
                     /gene="yigB"
                     /locus_tag="POB34_000262"
     CDS             complement(37073..37789)
                     /gene="yigB"
                     /locus_tag="POB34_000262"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HDI5917280.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(37789..38685)
                     /gene="xerC"
                     /locus_tag="POB34_000263"
     CDS             complement(37789..38685)
                     /gene="xerC"
                     /locus_tag="POB34_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDI5917281.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(38682..39389)
                     /locus_tag="POB34_000264"
     CDS             complement(38682..39389)
                     /locus_tag="POB34_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HDI5917282.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMVLLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(39386..40210)
                     /gene="dapF"
                     /locus_tag="POB34_000265"
     CDS             complement(39386..40210)
                     /gene="dapF"
                     /locus_tag="POB34_000265"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDI5917283.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(40247..40450)
                     /locus_tag="POB34_000266"
     CDS             complement(40247..40450)
                     /locus_tag="POB34_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDI5917284.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            40609..40751
                     /locus_tag="POB34_000267"
                     /pseudo
     CDS             40609..40751
                     /locus_tag="POB34_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            40913..41233
                     /gene="cyaY"
                     /locus_tag="POB34_000268"
     CDS             40913..41233
                     /gene="cyaY"
                     /locus_tag="POB34_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HDI5917285.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(41273..43819)
                     /gene="cyaA"
                     /locus_tag="POB34_000269"
     CDS             complement(41273..43819)
                     /gene="cyaA"
                     /locus_tag="POB34_000269"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDI5917286.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            44206..45147
                     /gene="hemC"
                     /locus_tag="POB34_000270"
     CDS             44206..45147
                     /gene="hemC"
                     /locus_tag="POB34_000270"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDI5917287.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            45144..45884
                     /gene="hemD"
                     /locus_tag="POB34_000271"
     CDS             45144..45884
                     /gene="hemD"
                     /locus_tag="POB34_000271"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDI5917288.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            45906..47099
                     /gene="hemX"
                     /locus_tag="POB34_000272"
     CDS             45906..47099
                     /gene="hemX"
                     /locus_tag="POB34_000272"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDI5917289.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQQETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPQADTSAA
                     APQGE"
     gene            47102..48298
                     /gene="hemY"
                     /locus_tag="POB34_000273"
     CDS             47102..48298
                     /gene="hemY"
                     /locus_tag="POB34_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HDI5917290.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            48977..49138
                     /locus_tag="POB34_000274"
     CDS             48977..49138
                     /locus_tag="POB34_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917291.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(49222..50457)
                     /locus_tag="POB34_000275"
     CDS             complement(49222..50457)
                     /locus_tag="POB34_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDI5917292.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDKFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVSK"
     gene            complement(50604..50680)
                     /locus_tag="POB34_000276"
     tRNA            complement(50604..50680)
                     /locus_tag="POB34_000276"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50644..50646),aa:Pro,seq:tgg)
     gene            complement(50723..50809)
                     /locus_tag="POB34_000277"
     tRNA            complement(50723..50809)
                     /locus_tag="POB34_000277"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50773..50775),aa:Leu,seq:cag)
     gene            complement(50830..50905)
                     /locus_tag="POB34_000278"
     tRNA            complement(50830..50905)
                     /locus_tag="POB34_000278"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50870..50872),aa:His,seq:gtg)
     gene            complement(50964..51040)
                     /locus_tag="POB34_000279"
     tRNA            complement(50964..51040)
                     /locus_tag="POB34_000279"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51004..51006),aa:Arg,seq:ccg)
     gene            complement(51143..52528)
                     /locus_tag="POB34_000280"
     CDS             complement(51143..52528)
                     /locus_tag="POB34_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI5917293.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(52719..53459)
                     /gene="rffM"
                     /locus_tag="POB34_000281"
     CDS             complement(52719..53459)
                     /gene="rffM"
                     /locus_tag="POB34_000281"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HDI5917294.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(53462..54814)
                     /gene="wzyE"
                     /locus_tag="POB34_000282"
     CDS             complement(53462..54814)
                     /gene="wzyE"
                     /locus_tag="POB34_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HDI5917295.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(54811..55890)
                     /gene="rffT"
                     /locus_tag="POB34_000283"
                     /gene_synonym="wecF"
     CDS             complement(54811..55890)
                     /gene="rffT"
                     /locus_tag="POB34_000283"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HDI5917296.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALCAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(55887..57137)
                     /gene="wzxE"
                     /locus_tag="POB34_000284"
     CDS             complement(55887..57137)
                     /gene="wzxE"
                     /locus_tag="POB34_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HDI5917297.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(57139..58269)
                     /gene="rffA"
                     /locus_tag="POB34_000285"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(57139..58269)
                     /gene="rffA"
                     /locus_tag="POB34_000285"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HDI5917298.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVIST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(58274..58948)
                     /gene="rffC"
                     /locus_tag="POB34_000286"
                     /gene_synonym="wecD"
     CDS             complement(58274..58948)
                     /gene="rffC"
                     /locus_tag="POB34_000286"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HDI5917299.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGVGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(58926..59807)
                     /gene="rfbA"
                     /locus_tag="POB34_000287"
     CDS             complement(58926..59807)
                     /gene="rfbA"
                     /locus_tag="POB34_000287"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDI5917300.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(59826..60893)
                     /gene="rffG"
                     /locus_tag="POB34_000288"
     CDS             complement(59826..60893)
                     /gene="rffG"
                     /locus_tag="POB34_000288"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDI5917301.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(60893..62155)
                     /gene="wecC"
                     /locus_tag="POB34_000289"
     CDS             complement(60893..62155)
                     /gene="wecC"
                     /locus_tag="POB34_000289"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDI5917302.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(62152..63282)
                     /gene="wecB"
                     /locus_tag="POB34_000290"
     CDS             complement(62152..63282)
                     /gene="wecB"
                     /locus_tag="POB34_000290"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDI5917303.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(63338..64384)
                     /gene="wzzE"
                     /locus_tag="POB34_000291"
     CDS             complement(63338..64384)
                     /gene="wzzE"
                     /locus_tag="POB34_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HDI5917304.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(64396..65499)
                     /gene="wecA"
                     /locus_tag="POB34_000292"
     CDS             complement(64396..65499)
                     /gene="wecA"
                     /locus_tag="POB34_000292"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HDI5917305.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(65739..66998)
                     /gene="rho"
                     /locus_tag="POB34_000293"
     CDS             complement(65739..66998)
                     /gene="rho"
                     /locus_tag="POB34_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDI5917306.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(67083..67184)
                     /gene="rhoL"
                     /locus_tag="POB34_000294"
     CDS             complement(67083..67184)
                     /gene="rhoL"
                     /locus_tag="POB34_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HDI5917307.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(67325..67654)
                     /gene="trxA"
                     /locus_tag="POB34_000295"
     CDS             complement(67325..67654)
                     /gene="trxA"
                     /locus_tag="POB34_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HDI5917308.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            67785..69050
                     /gene="rhlB"
                     /locus_tag="POB34_000296"
     CDS             67785..69050
                     /gene="rhlB"
                     /locus_tag="POB34_000296"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HDI5917309.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            69184..70668
                     /gene="gppA"
                     /locus_tag="POB34_000297"
     CDS             69184..70668
                     /gene="gppA"
                     /locus_tag="POB34_000297"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HDI5917310.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGAFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(70715..72736)
                     /gene="rep"
                     /locus_tag="POB34_000298"
     CDS             complement(70715..72736)
                     /gene="rep"
                     /locus_tag="POB34_000298"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HDI5917311.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            72953..73402
                     /locus_tag="POB34_000299"
     CDS             72953..73402
                     /locus_tag="POB34_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDI5917312.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGPFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            73601..73882
                     /gene="ppiC"
                     /locus_tag="POB34_000300"
     CDS             73601..73882
                     /gene="ppiC"
                     /locus_tag="POB34_000300"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HDI5917313.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(73969..75444)
                     /gene="ilvC"
                     /locus_tag="POB34_000301"
     CDS             complement(73969..75444)
                     /gene="ilvC"
                     /locus_tag="POB34_000301"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDI5917314.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     SAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            75594..76484
                     /gene="ilvY"
                     /locus_tag="POB34_000302"
     CDS             75594..76484
                     /gene="ilvY"
                     /locus_tag="POB34_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HDI5917315.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(76481..78025)
                     /gene="ilvA"
                     /locus_tag="POB34_000303"
     CDS             complement(76481..78025)
                     /gene="ilvA"
                     /locus_tag="POB34_000303"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HDI5917316.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(78028..79878)
                     /gene="ilvD"
                     /locus_tag="POB34_000304"
     CDS             complement(78028..79878)
                     /gene="ilvD"
                     /locus_tag="POB34_000304"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDI5917317.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(79943..80872)
                     /gene="ilvE"
                     /locus_tag="POB34_000305"
     CDS             complement(79943..80872)
                     /gene="ilvE"
                     /locus_tag="POB34_000305"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HDI5917318.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(80892..81155)
                     /gene="ilvM"
                     /locus_tag="POB34_000306"
     CDS             complement(80892..81155)
                     /gene="ilvM"
                     /locus_tag="POB34_000306"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HDI5917319.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(81152..82798)
                     /locus_tag="POB34_000307"
     CDS             complement(81152..82798)
                     /locus_tag="POB34_000307"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HDI5917320.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQLHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(82801..82851)
                     /locus_tag="POB34_000308"
     CDS             complement(82801..82851)
                     /locus_tag="POB34_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917321.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(82938..83036)
                     /gene="ilvL"
                     /locus_tag="POB34_000309"
     CDS             complement(82938..83036)
                     /gene="ilvL"
                     /locus_tag="POB34_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HDI5917322.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            83389..84909
                     /locus_tag="POB34_000310"
     CDS             83389..84909
                     /locus_tag="POB34_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDI5917323.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(84934..85272)
                     /gene="maoP"
                     /locus_tag="POB34_000311"
     CDS             complement(84934..85272)
                     /gene="maoP"
                     /locus_tag="POB34_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HDI5917324.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            85391..86230
                     /gene="hdfR"
                     /locus_tag="POB34_000312"
     CDS             85391..86230
                     /gene="hdfR"
                     /locus_tag="POB34_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HDI5917325.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(86326..86401)
                     /locus_tag="POB34_000313"
     tRNA            complement(86326..86401)
                     /locus_tag="POB34_000313"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(86366..86368),aa:Trp,seq:cca)
BASE COUNT        20974 a        23460 c        21927 g        20046 t
ORIGIN      
        1 aggagaaccc cgctgacccg gcggcgtgtt tgccgttgtt ccgtgtcagt ggtggcgcat
       61 tatagggagt tattccggcc tgacaagagg aaatttaaaa taattttccg accgcgcatt
      121 ttttattctt tatgcttatt tcagcgtcgg tttgtcggtt aaatgggcga tttctcgggc
      181 gaaattcgcc acctgctccc aatcggtata gacaacttct ttgcgcgtat ccgtttcacc
      241 gcctgacatc ttcataatta gcttgatcat aaaacggtcg taccagcgat aacgtgggta
      301 acgcagcgcc ccggcaatga ccgcgcagcg atcgggacgc cattgcgagt tcatcagaaa
      361 cttccgcgcg tagctgttgg tctgtggagt gcgcttctcc ggtttacgcg ccaccaggtt
      421 tacggagtaa aaggcgctcg gcatcgaatt cagccgcgtc gcatgctttt tgacaaattc
      481 ctggaacgca gagtggtagt gaccatagcg aatagaagca ccaatgacca cacggtcgta
      541 gttttcccac tgtggttctt caatgcggtg cacattggcg acatccgcct ggatccccag
      601 ttctttcagt tccgaagcca agtaggaggc aatctcgcgc gtttgtccgt cccttgttga
      661 gaaaagaatt aatgttttca cgtattactc catcattcac gccagaaagt cggggtaaag
      721 agcaccagca atgtaaagac ctcgagacga ccaaacagca tgttggcaat caggatccat
      781 ttagccaccg ggttcatact ggtaaagtta tcagcaacca cgccaagccc cggccccagg
      841 ttattcaatg tcgcaacaac cgaggcaaaa gcagaaaagt catccacgcc cgtggcgata
      901 atcgccagca tactgacaat aaacaccaat gcataggcgg agaaaaatcc ccaaacggct
      961 tcgaggatac gttccggcag tgcgcgattc cccagcttaa tgctatagac ggcgttcgga
     1021 tgcaccagtc gtttcagctc acggttcccc tgcttaaaca gcagcaggat gcggatcact
     1081 ttcaggccac cgcccgttga cccggcacaa ccgccgataa aagctgaaca taaaagcagt
     1141 accggcaaaa agagcggcca gcgggcaatg ctgtcagtcg taaacccggc ggttgtcgcc
     1201 atcgacacca cctggaaaaa cgcctggtta attgtcatca gcgccgaact gtagacattg
     1261 tgaaaccaca gtacgagggt acaaataacc accagcgtaa actgtacgcc gataaacatg
     1321 cgaaattccg gatcgcgcca ataaaccttc agactacgcc cacttaacag tgaaaagtgc
     1381 agaccgtagt tacagccgga gatcagcagg aagatagcaa tgatagtgtt aatagtcggg
     1441 ctgtcgaaat aaccgatact ggcatcatgt gtcgagaagc cgccaatagc gatagtcgca
     1501 aagctatggc cgatggcatc aaaggcatcc attccggcaa accacaacgc cagcgcacag
     1561 gcgacggtca gcaagacata aatcaaccac agggttttcg ccgtttccgc aattcgcggg
     1621 cgcattttgt tatctttcag cgggccgggc atttctgcgc gatagagctg catcccaccc
     1681 acgccgagga taggcagtat cgcaaccgct aacacgatga tccccatccc gccaaaccat
     1741 tgcagcatct ggcgataaaa gaggatggcg tgagggagcg aatccagccc caccagcgta
     1801 gtggccccgg tggtggtcag gccagagaaa gattcaaaaa acgcatcggt aatcgtgagg
     1861 ttcgggcttt ccgagaagat aaaagggagc gcaccgacac tacccagcac ggtccagaac
     1921 agcaccacta tcagaaaccc ctcacgggat ttcagttcgc ccttctcttt acggttcggc
     1981 caccacagca tagaaccaat ggcgagggcg acaaaaaagg tctgggtaaa agcgcggccc
     2041 gctccatccc ggtagatgag tgctaccagc ccagggataa tcatggtccc tgaaaataag
     2101 atgaccagta gtccaacgat tcgggtaatg gcgcgaaaat gcatctctgc cgcttcctta
     2161 gttctgcaaa acgaagtggg gattattctt caatcgctaa caattgcaat gaaccacggc
     2221 taaaatccgc cagctttgcg gaaaattcag ccactttcgc cgccggaagc gccacccgca
     2281 gcagaacgaa tgcctgataa tcactgttga taattttgcc gtcacactgc cccagcaacg
     2341 cttcaatgcc ggttaactga ctatattcac attgcaaagt atattcggtt aatggcgtct
     2401 tgcgttgggt cgttagctgg cgcagcgcct gattcacgcc gccgccatac gctttcacta
     2461 acccaccggt gccaagcaat atgccaccgt agtagcgcac taccacagcg gtaatttccc
     2521 cgacgccgct gcccattagc tgggcgagca tcggtttacc tgccgttcct gccggctccc
     2581 cgtcgtcaga gaaacccagc tgttgagaat catccggcgc acccgccacc cacgccacgc
     2641 aatggtggcg ggcatcgggg tgttctgccc gcaccgattc aacaaacgct ttcgccgcct
     2701 caacgccatc ggtatgcgcc aacagtgtta tgaagcggct ctttttgatc tcttcaacga
     2761 ccgtgaccgg tgccgcagga attaaccagc tttccatcac gccagtttca gatcccgggt
     2821 catgttttcc acgttgtttt cgtggatcac cacgttgtct tcgatacgaa tgccgccgaa
     2881 cggtttcagt gcttcaattt tctgccagtt gaagtgcttg ctgaactgcc cttcacgcca
     2941 cggtgccagt agcgattcaa tgaagtagat acccggttcg atggttaaca ccatgcccgg
     3001 ctggagaata cgggtgcagc gcaggtacgg atattttgcc ggtgccgcga ggtgtgtacc
     3061 gctatcatcc tgcataaaac cggctacgtc atgcacctgc aggcccagcg gatgaccgat
     3121 accgtgcggc ataaacggtc cggtgagatc gttttcgacc atcgcttctt cactcatatc
     3181 ggtgatgatt tgatgtttac gcagcagttt ggcgatgcgc tgatggaact ggatgtggta
     3241 atccacatag ctaacgcctg ctttcatggt ggcgatcagc gccagttgtt catcatttac
     3301 gtctttcacc agctgtgcgt agtcgttgtc gctttttgcc gaccaggtac gagtcaggtc
     3361 agccgcatag ccgttatatt cggccccggc atccagcagg aagctgcgca tctcttccgg
     3421 cgcctgatga tccagtttgg tgtaatgcag caccgcagcg tgttcgttaa gcgccacaat
     3481 gttgctgtaa ggtacgtcgg tatcacgatg accggtcgcg gtcagatagg caatgttgat
     3541 atcgaactcg ctcatgccag aacggaacgc ttcttctgcc gcacgatgac cgttgaccgc
     3601 cattttctgc gcttcacgca tacaggccag ttcgtactcg gttttgaagg agcgatagta
     3661 atgcaggtag tcgataaccc ctttcgggtt gatgttgctg gcctcaatac ccagttgcag
     3721 cgcacgctcc ggcaccggac cgatataacc gatattgccg cgcgcagcag gcagcaggct
     3781 accaatgcca tcggctttcg gcagcgcgat cacttccaca tcttcagtcc agaaggaggt
     3841 cggcagcggt tcgacgttgt gccagtaatc aaccggcaga taaaaccaca gtttcggctt
     3901 gttcacgcca tccaccagca accagcagtt tggcacctga gttaccggca cccacgcttt
     3961 gaattgcggg ttcactttaa acggataggg atgatcgtcg agaaaaacat tgaacagctc
     4021 gccggagtga ataagtaacg catccagctt gaagcgcgcc agcgcatcgc gagtccgttc
     4081 ttgtaaggta gctatatgat ttttatagag cgaggccagt gattccattt tttacccttc
     4141 tgtttttttg accttaagtc tccgcatctt agcacatcgt tcatccagag cgtgatttct
     4201 gccgagcgtg atcagatcgg catttcttta atcttttgtt tgcatatttt taacacaaaa
     4261 tacacacttc gactcatctg gtacgaccag atcaccttgc ggattcagga gactgacatg
     4321 ctttacaaag gcgacaccct gtaccttgac tggctggaag atggcattgc cgaactggta
     4381 tttgatgccc caggttcagt taataaactc gacactgcga ccgtcgccag cctcggcgag
     4441 gccatcggcg tgctggaaca gcaatcagat ctaaaagggc tgctgctgcg ttcgaacaaa
     4501 gcagccttta tcgtcggtgc tgatatcacc gaatttttgt ccctgttcct cgttcctgaa
     4561 gaacagttaa gtcagtggct gcaatttgcc aatagcgtgt ttaatcgcct ggaagatctg
     4621 ccggtgccga ccattgctgc cgtcaacggc tatgcgctgg gcggtggctg cgaatgcgtg
     4681 ctggcgaccg attatcgtct ggcgacgccg gatctgcgca tcggtctgcc ggaaaccaaa
     4741 ctgggtatca tgccgggctt tggcggttct gtacgtatgc cacgtatgct gggcgctgac
     4801 agtgcgctgg aaatcattgc cgccggtaaa gatgtcggcg cggatcaggc gctgaaaatc
     4861 ggtctggtgg atggcgtagt caaagcagaa aaactggttg aaggcgcaaa ggcggtttta
     4921 cgccaggcca ttaacggcga cctcgactgg aaagcaaaac gtcagccgaa gctggaacca
     4981 ctaaaactga gcaagattga agccaccatg agcttcacca tcgctaaagg gatggtcgca
     5041 caaacagcgg ggaaacatta tccggccccc atcaccgcag taaaaaccat tgaagctgcg
     5101 gcccgttttg gtcgtgaaga agccttaaac ctggaaaaca aaagttttgt cccgctggcg
     5161 cataccaacg aagcccgcgc actggtcggc attttcctta acgatcaata tgtaaaaggc
     5221 aaagcgaaga aactcaccaa agacgttgaa accccgaaac aggccgcggt gctgggtgca
     5281 ggcattatgg gcggcggcat cgcttaccag tctgcgtgga aaggcgtgcc ggttgtcatg
     5341 aaagatatca acgacaagtc gttaaccctc ggcatgaccg aagccgcgaa actgctgaac
     5401 aagcagcttg agcgcggcaa gatcgatggt ctgaaactgg ctggcgtgat ctccacaatc
     5461 cacccaacgc tcgactacgc cgggtttgac cgcgtggatg ttgtggtaga agcggttgtc
     5521 gaaaacccga aagtgaaaaa agccgtactg gcagaaaccg aacaaaaagt acgcccggat
     5581 accgtgctgg cgtctaacac ttcaaccatt cctatcagcg aactggccaa cgcgctggaa
     5641 cgcccggaaa acttctgcgg aatgcacttt tttaacccgg tacaccggat gccgttggta
     5701 gaaattattc gcggcgagaa aagctccgac gaaaccatcg cgaaagttgt cgcctgggcg
     5761 agcaagatgg gcaagacgcc gattgtggtt aacgactgcc ccggcttctt tgttaaccgc
     5821 gtgctgttcc cgtatttcgc cggtttcagc cagctgctgc gcgacggcgc ggatttccgc
     5881 aagatcgaca aagtgatgga aaaacagttt ggctggccta tgggtccggc atatctgctg
     5941 gatgtcgtgg gcattgatac cgcgcatcac gctcaggctg tcatggcagc aggcttcccg
     6001 cagcggatgc agaaagatta ccgcgatgcc atcgacgcgc tgtttgatgc caaccgcttt
     6061 ggtcagaaga acggcctcgg tttctggcgt tataaagaag acagcaaagg taagccgaag
     6121 aaagaagaag acgccgccgt tgaagacctg ctggcagaag tgagccagcc gaagcgcgat
     6181 ttcagcgaag aagagattat cgcccgcatg atgatcccga tggtcaacga agtggtgcgc
     6241 tgtctggagg aaggcattat cgccactccg gcggaagcgg atatggcgct ggtctacggc
     6301 ctgggcttcc ctccgttcca cggcggcgcg ttccgctggc tggacaccct cggtagcgca
     6361 aaatacctcg atatggcaca gcaatatcag cacctcggcc cgctgtacga agtgccggaa
     6421 ggtctgcgta ataaagcacg tcataacgaa ccgtactatc ccccggttga gccagcccgt
     6481 ccggttggcg acctgaaaac ggcttaagga gtcacaatgg aacaggttgt cattgtcgat
     6541 gcaattcgca ccccgatggg ccgttcgaag ggcggtgctt ttcgtaacgt gcgtgcggaa
     6601 gatctctccg ctcatttaat gcgtagcctg ctggcgcgta acccggcgct ggaagcggcg
     6661 gccctcgacg atatttactg gggttgtgtg cagcagacgc tggagcaggg ttttaacatc
     6721 gcccgtaacg cggcgctgct ggcagaagtg ccgcactctg tcccggcggt taccgtcaat
     6781 cgcttgtgtg gttcatccat gcaggcactg catgacgcag cacgaatgat catgaccggc
     6841 gatgcgcagg catgtctggt tggcggcgtg gagcatatgg gccatgtgcc gatgagtcac
     6901 ggcgtcgatt ttcaccccgg cctgagccgc aatgtcgcca aagcggcggg catgatgggc
     6961 ttaacagcag aaatgctggc gcgtatgcac ggtatcagcc gtgaaatgca ggatgccttt
     7021 gccgcgcggt cacacgcccg cgcctgggcc gccacgcagt cggccgcatt taaaaatgaa
     7081 atcatcccaa ctggtggtca cgatgccgac ggcgtcctga agcagtttaa ttacgacgaa
     7141 gtgattcgcc cggaaaccac cgtggaagcc ctcgccacgc tgcgtccggc gtttgatcca
     7201 gtaaacggta cggtaacggc gggcacatct tctgcacttt ccgatggcgc agctgccatg
     7261 ctggtgatga gtgaaagccg cgcccatgaa ttaggtctta agccgcgcgc tcgtgtgcgt
     7321 tcgatggcgg tcgttggttg tgacccatcg attatgggtt acggcccggt tccggcctcg
     7381 aagctggcgc tgaaaaaagc ggggctttct gccagcgata tcggcgtgtt tgagatgaac
     7441 gaagcctttg ccgcgcagat cctgccgtgc attaaagatc tcgggctaat ggagcagatt
     7501 gacgagaaga tcaacctcaa tggtggtgcg atcgcgctgg gtcatccgct gggctgttcc
     7561 ggtgcgcgta tcagcaccac gctgctgaat ctgatggaac gcaaagacgt tcagtttggt
     7621 ctggcgacga tgtgtatcgg tctgggtcag ggtattgcga cggtgtttga gcgggtttaa
     7681 ccacttgccg gatgcggcgt aaacgcctta tccggcctac ggttcggact atttgtaggc
     7741 acgataagac gcgacagcgt cgcatcgggc atggtttgcc agatacgtca tagatctaaa
     7801 tgccactgaa taaagtttta gttgccgttc ttcccgcctg tcaggggcgg gtttttttat
     7861 atctcagata aatgcaaacg catcgccaaa caggcgatct tcccgcgcat tacgctcact
     7921 acaaaacagg tcacgggcta ttttcgccat ctcaaaacgt ccggcaatat agatatcatg
     7981 ctctgccagc gtaccgtgat cctgcaatac cgccgttaac acggtgccag tacgcccacg
     8041 ccagcccgct tccggttgtt caaccaccgg caccacttgc agaccagggt gctttaacga
     8101 aagcgcttca agctcgcaga gatcatacag atgctgctct tcacgcccgc cccagtaaat
     8161 ggtgatatca cggtttgggt tacgcgccaa cgctgtcagc aaaatcgagc gggcataaga
     8221 gaacccggtg ccgcccgcaa tcaaaatcat cggacgctct tcatcatcgc gcagccacgc
     8281 ttctccgtgg ggaatatcga ccacgatttg atgatctttg aggatgcggt ccatgactgc
     8341 tttcgcgtaa aggttgattt cagaagcgcc aatatgcagc tcgataaacc ctttttcatc
     8401 cggcgtcgag gccattgaga acggacgttt gtcgcgctca tccatcacta ccatcaaata
     8461 ctgaccagca cgaaaagaaa aggccgcgtc tggcacgatg cggacacgat atacggtatc
     8521 cgtgatagct tctaccgagg tcactttaca gcttaaggtt gtcatgcgct ttctctgtcg
     8581 gatcgataaa tagggcaaaa caaacgcaca tcaggcgctt ttaccgttgt taaaaatagc
     8641 cagttcatcc cagatggcgt caatatgcgc gacaacatct ggatcttttt tgatgggacg
     8701 tccccattca cgctgggttt cccccggcca tttattcgtg gcatccagcc ccatttttga
     8761 acccagcccg gaaacaggcg aggcaaaatc cagataatca ataggcgtat tttctaccag
     8821 aacagtatcc cgcgccggat ccatacgggt ggtaatcgcc caaatcacat cgttccagtc
     8881 gcgtgcgttg acgtcatcat cgcaaacgat cacaaactta gtgtacataa actggcgtaa
     8941 gaacgaccag acgcccatca tgacgcgctt cgcgtgtccg gcgtactgtt tttttatcgt
     9001 cactaccgcc agacgataag aacagccttc cggcggcagg taaaaatcga caatttccgg
     9061 gaactgtttt tgcagaatcg gcacaaacac ttcgttcagt gcgacaccca gcaccgcggg
     9121 ttcatccggc ggacgaccag tataggtgga gtggtaaatc gcatcttcac gctgggtaat
     9181 atgcgtcacg gtaaataccg ggaaactatc gacttcgtta tagtaaccgg tgtggtcgcc
     9241 atacggccct tccggagcag tttcgccttg ttctatatac ccttccagca caatctccgc
     9301 actggcgggc acttcaaggt cattggagat acacttcacc acttcggtct tggtgccacg
     9361 tagcaatccg gcaaacgcat actctgaaag cgtatccgga acgggggtga ctgcaccgag
     9421 aatcgtggcg ggatcggcac ccagcgccac agaaaccggg aaacgttcgc ccggatgcgc
     9481 cgcacaccac tcctgataat ccagcgcgcc gccgcgatgc gacagccagc gcataatcag
     9541 tttgttttta ccaatcagct gctgacgata aatgcccaga ttctgccgct ctttatgcgg
     9601 gccgcgcgtt acggtcagcc cccaggtaat cagcggcgcg gcatcttccg gccagcaggt
     9661 cataatggga atgcgattga gatcgacgtc atcgccagag acgatttttt gttggcaggg
     9721 cgcaccacgc agtcgctttg tcggcatgtt caatacttgc ttaaactgcg gcagtttatc
     9781 aaacaggtcg cggaaacctt ttggcggctc cggctctttc agaaacgcca ataatttacc
     9841 aacttcacgc agcgccgaaa catcttcctg ccccatgccc atcgccacgc gctttggcgt
     9901 accgaacagg ttgcacagca ccggcattga gtagccttta gggttttcga acaacagcgc
     9961 aggcccaccg gcacgcaaag tgcggtcagc aatttcagtg atttccagat gcggatccac
    10021 cgggagcgtg atacgtttta gctcaccctg ctgttcaagc agcgtcagga agtcgcgtaa
    10081 atcgttatat ttcatggcgt ccattgtagc ctcttaatct acgcccatta tacggcgttc
    10141 atctttgcga tgctgtaaat ttgttaaatt agcgtgaact ctgacggtat aacgcaaacc
    10201 ggggaatata attaacttag cgtaaagctt ttgctatcct tgcgccccga ttaaacggat
    10261 aagagtcatt atgcaatcct ggtatttact gtactgcaag cgcgggcaac ttcaacgtgc
    10321 ccaggaacac ctcgaaagac aggctgtgaa ttgcctggca ccgatgatca ccctggaaaa
    10381 aatcgtgcgt ggaaaacgta ctgcagtcag tgagccattg tttcccaact acctgtttgt
    10441 cgaatttgat ccagaagtga ttcataccac gactatcaac gcgacccgcg gtgtcagcca
    10501 cttcgtgcgc tttggcgcgt cgccagcgat agtcccatcg gcggtgattc atcagctatc
    10561 ggtatataaa ccgaaagaca ttgtcgatcc ggcaacccct tatccgggtg ataaggtgat
    10621 tattaccgaa ggcgcgttcg aaggctttca ggccattttc accgaacccg atggtgaggc
    10681 tcgctccatg ctattgctta atcttattaa taaagagatt aagcacagtg tgaagaacac
    10741 cgagttccgc aaactctaaa acgcaatccc aaacagtgtt ttgacattag catccgtggt
    10801 ggcagccagc catgcggcat cttctccacg ccagtgcgca atacgttgca aaatatgggg
    10861 cagatgggct ggctcgttgc gccgggatga tggctttggc gtgagatcgc gagggagcag
    10921 atacggcgca tcagtttcga tcagtaattt ttccgccgga atcaacggca aaagttcccg
    10981 cagctccagt ccgcgtcgtt catcgcaaac ccaaccggta atgccgatat aaattccatg
    11041 cgccacgcac gcctgcatct cttcgcgtgt gccggtaaag caatgaagaa ccgcaccagg
    11101 cagtttatcc agccacggct ccagcaatgt cataaaccgc tcgtgggcat cgcgacagtg
    11161 cataaatacc ggcatgttta aatctgcggc aatgcgtagc tgggcaacaa aagcgcgttc
    11221 ctgctcttcc ggcgtcgaaa agttgcggtt aaagtcgaga ccacattcac caatcgccac
    11281 cacttctggc tgcgcggcca gctcaataat cgcttcttca gtcgcagctt gccactggct
    11341 gctgtcgtga ggatgtacgc ccgccgttga ccaacaggac gaatactgac gcgccagctt
    11401 ttgcgcctgc tggctttcac gcaggttagt gccggtgatg agtagcccat taactcccgc
    11461 gtcaaaagcg cgcgctacaa catcatcacg gtctttcgca aattgcgaac tggtcaaatt
    11521 aacgccgata tcaaacatcc tgtactccat atgacaaccg ccctgacggg cggttgaatt
    11581 tattcttcag ttttttcgct ttctgcttca gcgtcgtttt cctcttcccg atttcgccct
    11641 ttaccaacgt aaaagcgaga gaagaagaca ccgatttcaa acagacagta catcgggatc
    11701 gccaacagcg tttgcgagaa gacatccggc ggcgtcagca acatcccgac aacgaatgca
    11761 ccaaccagca catacgggcg ttttttgcgt aagtcttctg gcgaggtaat ccccatccag
    11821 cacagcagca caattgctac cggcacttca aaggagacac caaacgccat aaacagcgcc
    11881 ataacgaagc ttaaatagct ggcgatgtcg gtggatacct gcaccccttc cggcgcggta
    11941 ttggcaagga agccaaatgc cagcggaaag accacaaagt aggcgaatgc catgccgata
    12001 taaaacagca gagagctgga aaccagcagc ggcaccacca ggcgacgttc atgcttatac
    12061 agcgctgggg cgataaatgc ccacacctga tagagaatca ccggcgctga cagaatcagc
    12121 gacaccataa aggtcagctt gatcggcgta aagaacggcg aggccacgtc ggtggcgatc
    12181 atcgttgaac cttgcggcaa ctgcttgatc aatggcgcgg ataccaggtg atagatgtca
    12241 ttggcgaaat agaccagaca caggaatatc acgatcaccg cgataatgca gttcagcaga
    12301 cgcttacgca gctcaatcag atgcgtgata agcggttgag tatcttctac agacatgttt
    12361 acggtttatc actcgacgaa ggggaaggtg cagcggtttt cggttcagcg tccgcagcag
    12421 gttttaccac cggctctggc gtggtttctg gcttctgttc cggcgaactg gcctgcgttt
    12481 gtgcagcggc aggcgttacg ccctcatgcg cagcttcatt atctttcacc accgggttat
    12541 ggatggtgtg cgcttcatcg ctcgcctttt caggatcgtt tgcaacgtag gaacgcttca
    12601 tcgactccgc ggcctggcgt agttcatcca tcgacgcttt cagttcgggt gtcaggttag
    12661 tgaggctcgc cttttcaacc tttttcagac tgtcctgaaa ctcctggagt tttaactcct
    12721 gggtcagttc gttctgcacc gttgtcgcca gtgaacgcaa cgcgcgaatc cagcccgcta
    12781 ccgtttttac cgccacaggc agtcgttgcg gccccagaac gacgaggccg atgatgaaca
    12841 ccaatagcag ttcgctaaaa ccgatatcaa acacggatta cacctgctct ttatcgtggc
    12901 gcttcgcgtc ttctgtttta gcctgttcct gattcgtatc cgcctgctta tcggcgatag
    12961 ttttcgcagt aaaatcagca tcctgactgg ttttatcctg ctttggttca tcatcgctca
    13021 ttgctttttt aaagcctttg atcgacgcac caagatcgga accgatggag ccgagctttt
    13081 tggtgccaaa aagcagtaca acgatgacgg caataatcaa taactgccaa atactgatac
    13141 cacccataca tgttcctctg tggtagatga tgattaaaca aggccgcatt atacgttaca
    13201 cggcccgcct tgagcgatga aaaaatcagc gtgttttgcg ccaaccgaca aaccaggcga
    13261 tcagaccacc tgccattaac cagccgggca tcagccccca ttcaggtcgg ctgaccaaca
    13321 agaatgtgcc acttaatact aacgtagcgc caattccgag aaaataacgc gattgtccct
    13381 gacgtacatg atttgactga agctcgcggg caatcttatc aacactgtgc tgtaaatact
    13441 tgccctggcg caaactgtcg taaaccagtt caggcagttc tggcattttt tcgacccaga
    13501 acggcgcttt ttctttaaat gctctcacca gcgcaggaat accgacctga tctttaatcc
    13561 acgactccag gaaaggcttc gccgttttcc ataaatcgag ttgcggataa agctggcgtc
    13621 ctaccccttc gacgtagagc agggttttct ggagtaacac cagttgcggc tgcacttcca
    13681 tattgaagcg acgcgccgta ttaaacagat ttaacagtac atgtccaaac gaaatttcgg
    13741 ccagcggttt ctcaaagata ggttcacaga ccgtacgaat ggcaaattcg aactcttcaa
    13801 cgttggtatc tggtggcacc cagcctgaat cgacgtgtag ctctgccact ttgcgatagt
    13861 cgcgattaaa gaaggcgata aagttttctg ccagatagcg tttatcttct ttgtttagcg
    13921 agccaacaat cccgcaatca atgccgatat atttcgggtt ttccgggtgt tcatagctta
    13981 cgaagatgtt gccagggtgc atatcggcat ggaaaaagct gtcgcgaaag acctgagtga
    14041 agaacacctg cacgccgcgt tccgccagca atttcatgtt agtgccgttt ttctccagcg
    14101 ccgcaacgtc agacaccgga atgccgtaaa tgcgctccat caccatcatc ccttcactac
    14161 aatagtcagg gtaaacttcc gggatgtaga gcatcgggct gtcttcaaaa ttgcgccgaa
    14221 gctgaatggc gttggcagat tcccgcagca aattcagttc atcaatcaat gtcttttcgt
    14281 actcgcgcac cacttcggtt gggcgcagac ggcgaccatc cggcagcaaa cgcggcaccc
    14341 agcgagccag acggtagata agtttcagat ccgctttaat aaccggcaaa atatccgggc
    14401 ggatgacttt aatcaccacc tctttaccat tcgatttcaa tcgcgcggta tgaacctggg
    14461 cgatagaagc agaagccagc ggcttgattt caaaatcgtc aaaccacgct tctaccggca
    14521 agccgcccat tgcagcttca atctgctgct tcgccagctt gccatcaaac ggagcaactt
    14581 tgtcctgcaa taacgccagc tgatcggcaa tatgcggtgg aaaaagatcg cggcgggttg
    14641 ataacatttg cccgaacttg atccaaaccg gccccagttc ttgcagggcc agtcgtagtc
    14701 gctcacctaa aagtttgtct ttatgccgat ttggcatcca gaataatgag tatcgccata
    14761 gccgtagcgg cagggtgata cgcattttgg ggatcagttc atcaagtccg tagcttaaaa
    14821 aagtgcgaat gatgaaatat aggcgccgta cttcacctgg cgtcatttag cctccagttt
    14881 ttccagccgt ttggtcaggg catcaacagc acgctcgacg gcagccgtct cttccgcaaa
    14941 ccaggccact tcaagcggac cgggtgccat acgccactct tcagtaatgg cttccgccac
    15001 ataacgttgc tggcgcttaa tgccgtgatg caggaacttt gcgcctccgc gcatggcttt
    15061 gctgattcct tcagcggcga tatcaccggt ataaggggcc agcagttccg cagggtcgaa
    15121 ctctgccaga tctgccagcg caacgaagtt ttgcaccacc tgaatatcgc cctgcacttc
    15181 cagctcacca ctgcgaatca gtgcggtaag ctgctggcga tcgcgaagtt tcggcaacac
    15241 actggcgtag gcgataacgg tgcagtcagc atcgcctgcc cattcgccca gtacatcaac
    15301 ctggcgttcg ctgaacacca gaatcaatga cgtcgaaaag ccttttacct ccacgcgcaa
    15361 tactttaccc agcagacgcg agcgggccgt tttcagcgcg ggtgagcgat acaggaaggt
    15421 gttgagcaga ctttcaattc ctgccgtcac taaaggttta aaaggcattt ccggtctcct
    15481 gtcagaactt ataaccacga tgcagcgcca caacccctgc cgtcagattg tagtagtcga
    15541 cactttcgaa tccggcatcc tgcatcatgg ctttcagggt atcctgatcg ggatgcatac
    15601 ggatggattc tgccagataa cggtagctgt cggcgtcgtt cgcgaccagt gagccaatac
    15661 gcggcagcac atggaaggag tatgcatcat aggctttgct cagcggctcg ataattggct
    15721 tcgagaactc aagcaccagc aggcggccgc cgggtttcag cacgcgatac attgaacgca
    15781 gtgctttatc tttgtcggtg acgttacgca gaccaaacga aatggtgatg caatcaaagg
    15841 tgttatccgg gaacggcagc gcctcagcgt tcgcctgaac atactcaacg ttgccaatca
    15901 caccgatatt acgcagcttc tcgcggccca ttttgagcat ggattcattg atatcagcaa
    15961 ggaccacttt gccagtttct ccgaccaggc gggagaattt cgctgtcagg tcgccggtgc
    16021 caccagccag atccagcacg gtctgcccac ggcgtacgcc gctgcaatca atcgtgaatc
    16081 gcttccacaa acgatgaata ccaaatgaca tcaaatcatt catgacatcg tattttgatg
    16141 ccacggaatg gaaaacgtgg gcgaccatat ccgctttttg ttccttcgcg acggtctgaa
    16201 aaccaaagtg cgtcgtttct tgtgacttat ccaccatctc aatgcctgct catcaaaaaa
    16261 ttgttccaga agtgtaacag attgggcgtc gatgccctag atttctaccc ggcttaacta
    16321 cccctaatgg gctagcgcga ctgctgatta tattcatcat cgcgttgata agcttcatca
    16381 ttcggctgct ccggaaccga ccgaagtcga tactcttcat cctggctcac cgcctgttca
    16441 gccaaatccg gattaatctc gcgtttaatt tctactccta aaccgcgaaa cgcttctgcc
    16501 tgcgccagca catttccgcg acctgaagag agttttttca ttgcctggcg gtagttatcc
    16561 tgcgctttgt cgagactttg accaatcgcg gacatgtcat cgatgaacaa acgcatcttg
    16621 tcgtacagct tgctggcacg atcggcgatt tgctgggcgt tgcggctttg atgctcataa
    16681 cgccacaggt tggcaatagt acgcaacgcc accagcagcg tagtcgggct aaccagcatg
    16741 atattgtttt tcaacgcttc ggtgatcagc tccggctggc ggtcaagcgc cagtaaaaaa
    16801 gcgggttcaa cgggaataaa catcagcacg taatccagag ttcgcagccc cggcagctgt
    16861 tgataatctt tgcgtcccag caaacggata tggttacgca ccgacgcgat atgttcctgt
    16921 agcgcgcttt cgcgggtgta gtcgtcttcg gcgttaaaat agcgttcata ggcgaccagc
    16981 gtcattttgg cgtcgatcac cacatctttt ccctgcggca gccgcacgat gacatccggc
    17041 tgcatccgcg agcgggcgtc attttcgatg ctgacctggg tttcatattc atacccttca
    17101 cgcagaccgg aagcctccag cacccgcgtc aatactacct cgccccagtt gccctgggtt
    17161 ttattgtcgc ctttcagcgc gcgcgtcagg ttgatcgctt cctgggccat ttgcgcgttg
    17221 agttgctgga gattgcgaat ttcgtgggtc agggtatggc gttcttgtgc ttctttaccg
    17281 aagctgtcct gaacctgacg gcggaaaccg tccagttgtt cacgtagcgg cgacaacagg
    17341 ctgttcagac tctgacggtt ttgctcatca acccggcgat tgctgtgctc aaaaatacgg
    17401 ttggcgaggt tttcaaactg ctcactgagg cgctgctcgc tgttaatcat ctggcgaatt
    17461 ttatcgtcag catgttgctg tgcggcttcc atccgcgtgg ttacttcacg cagatcggcc
    17521 tccagagagg tgttaatact ttgcaggctg cgcacttcgt tattgagtaa ctcgcactct
    17581 gcacgccagt gctcgctttg ggtaatttgt tgttttgccg cgcttaactc cgcgaccatc
    17641 tcttcacgtt cagctaattg ctcggctttt tgctgcgcat attgataact ggcaaacagc
    17701 cagccaattg ccacacccac caacgcaata actgcgtaaa ccatgattga gaaatccaca
    17761 acgcctcctg acgcaacaac ttacccgcac aaaatagaat tgtgctgtac aaacgtccag
    17821 ttggaaaggc atttcctgcg aggcgctacg caaaaataca aaaggccgaa cgcgtcggcc
    17881 ttcagacagg agaagagaat tacagcagac gacgcgccgc ttccaccacg attttcaccg
    17941 cttggctttc agtttgtttc atcgtctcag cattcgggat ctcttgctgg gtgcggttaa
    18001 cgataacacc cgctaccata ccggcacgca ggccctgact tgcacacatg gtcagcaggg
    18061 ttgcagattc catttcatag ttcattacgc ccatcgcctg ccactcttcc atagaacctt
    18121 taaagtgacg aactacgcga ccagagtaag tatcgtaacg ttcctgacct gggtagaagg
    18181 tatcagaaga agctgtcacg ccaacgtgag ttgtcgcgcc aatggatttc gcagcttcaa
    18241 ccagcgcagt cgtacattcg aaatcagcga cagccgggaa ttccagcggt gcgaagtgca
    18301 ggctcgcgcc atccagacgg acagacgccg tggtaaccag gacatcaccc acattaatat
    18361 gcggctgaat agcgcccgtt gtaccgatac gcaggaaggt gcgaatgccc agctgtgcca
    18421 gctcttcaac agcaatagag gtagacgggc cgccgatacc ggtagagcag acgataacag
    18481 gtttaccatc cagctctgca cgccaggtag tgaattcgcg gtgagatgcc agcttaaccg
    18541 gcttatccat cagcgcggcg atcttttcca cacgatccgg gtcgccaggg acgatggcaa
    18601 gcgtagcccc ttgtaaatcg tttttagtga ggccgagatg aaaaacatca gacttggaca
    18661 tatacaactc ctctgtgaat cggtttagtc agaagaagca aaaagacact ttaccgaagg
    18721 gtttaacatt ttttcgtgat actcatcacc atgacgcaaa tgcgttgcat aaatttaccc
    18781 accaaaagtg attcaaatca cataaatcac tcttcattct gcaggactgc ataaaagatg
    18841 agccgattca ggcaatgtga tttgttgcat gctgactata gttaacattg cgcaacattt
    18901 ttataagggt acggagaata ccatggcaac aacacaacaa tctggatttg cacctgctgc
    18961 atcgcctctc gcttcgacca tcgttcagac cccggacgac gcgattgtgg cgggcttcac
    19021 ctctatccct tcacaagggg ataacatgcc tgcttaccat gccagaccaa agcaaagcga
    19081 tggcccactg ccagtggtca ttgtagtgca ggaaattttt ggcgtgcatg aacatatccg
    19141 cgacatttgt cgccgtctgg cgctggaggg gtatctggct atcgcacctg aactttactt
    19201 ccgcgaaggc gatccgaatg attttgccga tatccccacg ttgcttagcg gtctggtagc
    19261 aaaagtgcct gactcgcagg tgctggccga tctcgatcat gtcgccagtt gggcttcccg
    19321 caacggcggc gatgttcatc gtttaatgat caccggattc tgctggggtg gacgtatcac
    19381 ctggctgtat gccgcgcata atccacagct aaaagctgca gtggcgtggt acggcaaact
    19441 gacgggcgac aagtcgctga attcaccgaa acaacctgtt gatatcgcaa ccgatcttaa
    19501 cgcgccggtt ctcggcttat atggtggtca ggataacagc attccgcagg agagcgttga
    19561 aaccatgcgc caggcgctgc gggctgctaa tgcgaaagca gagattatcg tctacccgga
    19621 tgccgggcat gcattcaacg ccgattatcg cccgagctat catgccgaat ctgcgaaaga
    19681 tggctggcag cgtatgctgg agtggtttaa gcagtatggt gggaagaagt cgctgtaatg
    19741 agaaaagacc gggtggtatt accacccggt ttggatttac ccccgacgca aattctgcgc
    19801 cgcctgcacc atgttcgcca gtgccgcgcg ggtttctggc cagccgcgcg ttttcaggcc
    19861 acagtccggg ttgacccaca ggcgctctgc cggaatgcgt tttgccgctt tcttcagcaa
    19921 ggcttcaatc cattccacgc tcggtacgtt tggcgagtga atgtcataga cgccaggacc
    19981 gatttcattt ggataatcaa actcttcaaa cgactccagc aactccatgt cggaacgcga
    20041 ggtttcgatg gtgatgacgt ctgcgtccag cgccgcaatc gaatccatga tgtcgttgaa
    20101 ctcgcaataa cacatgtgag tgtggatttg tgtgtcatct ttcgccacgg cggcgttgat
    20161 acggaaggcc tctacgcccc actggagata ggcgtcccag tcgctacgac gcagcggtaa
    20221 accttcgcgc agcgccggtt catcaatctg gatgatgcca atccctgccg cttccagatc
    20281 ggccacctca tcacgcagcg ccagcgcaat ctgtttggcg atggtttcac gactgacatc
    20341 ttcacgcggg aacgaccagc agagtatggt caccggcccc gtcagcatcc ctttcaccgg
    20401 tttgtcggtc agcgattgcg catacttcgc ccactccacg gtaatcggtg ccgggcggct
    20461 aatgtcacca atgacaatcg gtggcttcac gcagcgggaa ccgtagctct gtacccaacc
    20521 gttttgcgta aagacaaatc cgtcgaggtg ctcgccaaag tattccacca tgtcattacg
    20581 ctcggcctcg ccatgtacca gcacatccag tcccaaacgt tcctgctcaa caatggcctg
    20641 cttgatatgt tccgcaatgc ccgtgcggta gttgttggcg tcgagattgc cctttttgaa
    20701 atccagacgc agggtacgaa tttccgtggt ttgcgggaag gaaccaatcg tggtggtcgg
    20761 ccacgctggc agtttaaaac gcgcacgctg ggcttcagca cgcacttcat agacattcgc
    20821 acgctggctg tcctgggcgg tgatcgccgc cagacgcttt tctaccgccg gattatgtac
    20881 acgagtagag tgacgacgcg cctgaattgg ggcgctccac tctgccagag ctgccgtgtc
    20941 accactgttc agcgcatcgc gcagcagtgc cagttcatgg catttttgta gagcgaaggc
    21001 aaaccagctt ttcacttctg catcaagacg cgtttccacg ctcaggtcga tggggctgtg
    21061 cagcaacgag caggaagatg ccacccacaa atcacgtttg ccgacaatgt ccttaatttg
    21121 cgcatatttc tcagtaagat cggcgcgcca gacgttacga ccattgatca gtcccgcaga
    21181 cagcagccag tcagaaggca ggcgcttgtg cagttcaaca acgtcatctt taccatgtac
    21241 aaggtcaaca tgcagaccct gaacaggcag cgcagtaatc gtgtcgagat ttggcgttac
    21301 gccttcaaaa taggtggtca gcagcagttt cacctgtccc tggagcgcgt cgtaagcggg
    21361 tttgtatgcg tccagccacg cctgtggtag ttccagtacc agcgcgggtt catcaatctg
    21421 tacccactcg atgccgcgtt tcgccagttc tgccagcact tgctgataaa ccggcagaat
    21481 gtcgttcagc aggctaaggc ggtcaaactg ttcacctttc actttcccca gccacaggta
    21541 agtaaccggc cccagcagca caggcttaac gttgtggccc agcgccagcg cctcgtccac
    21601 ttcctccagc agctgcgtcc aggtcagttt gaactgttgg cctttaacga attccggcac
    21661 catgtagtga tagttggtgt taaaccattt ggtcatttcc gctgccgccg caggttcgcc
    21721 agtcggcgca cgtccacgac caatacggaa cagggtgtcg atatctaccg aaccgtcttt
    21781 gttctgatga cgtggcggaa cattacccag cagcagactg gtggtcagaa catgatcgta
    21841 ccaggcaaaa tcgcccaccg gcagcaggtc gatacccgct tgcttttgtt gatcccagtg
    21901 acgagcacgc agttcacgcc ctactgtcag cagttcttca cgcgtggagt tccccgccca
    21961 ataactttct tgcgcttttt tcagctcgcg acgcaggcca acgcgaggga aaccgagggt
    22021 gtgattaaga attgtcatta tatgcccctt atggaatttt tagtatttag ccgtccagat
    22081 gtttacacat ccataattgg cggttactgt atattcctca agcgcaaatt attcatgccg
    22141 aagtgaagga ctttcatgat cgaagtaaaa cacctgaaaa cgctacaagc gttgcggaac
    22201 tgcggctctc tcgcagccgc tgcggcgacg ttgcatcaga cgcaatccgc cctgtctcac
    22261 cagtttagcg atctggaaca acgccttggc ttccggctat ttgtgcgtaa gagccagccg
    22321 ctacgcttta caccgcaggg agaaatcctg ttgcaactgg caaaccaggt gctgccgcaa
    22381 attagccagg cactgcaagc ctgcaatgaa ccgcagcaga cgcgtctgcg cattgccatt
    22441 gagtgccata gctgtattca gtggctgacg cccgcgttag aaaatttcca taagaactgg
    22501 ccgcaggtag agatggattt taaatcgggc gtgacgtttg acccgcagcc cgcgttgcaa
    22561 cagggcgagc tggatctggt gatgacctcc gatatcctgc cgcgcagtgg cctgcattat
    22621 tcgccgatgt tcgactatga agtgcgtctg gtgttagcac ctgaccatcc actggcggcg
    22681 aaaacgcgaa ttacaccgga agatctcgcc agcgagacgc tgttgattta tccggtgcag
    22741 cgtagtcgac tggatgtctg gcggcatttt cttcagccgg caggcgtcag cccgtcactg
    22801 aaaagcgtcg ataacacctt attgttgatt cagatggttg ccgcgcggat gggtattgcc
    22861 gcgctaccgc attgggtagt agagagtttt gagcgccagg gtctggtggt gaccaaaacc
    22921 ctgggcgaag gcttgtggag ccgactgtac gccgccgtgc gcgatggcga gcagcgtcag
    22981 ccgattacag aagcatttat tcgctcagcg cgcaatcacg cctgcgatca tctgccgttt
    23041 gtgaagagcg cggagcgacc cacttacgat gcacccacag tgaggccagg atcaccagcg
    23101 cgcctgtaat gaacgttggc cagtgcggct gttggtgcca gatagccagg tttaccagca
    23161 gccctgccgg aacgtgcata ttattcataa tgcccagcgt tccggcgtcc acctgcgtcg
    23221 cgccgtagtt ccacataaag tagccaatcc cggaagccac cacgccaaga aacaccagaa
    23281 tgccccattg caacgtggtt tgcggcattt tctgcgcatt tcccagcaag aaccatgcaa
    23341 ttaccgccac caggaacgcg ccaagataga accacgcaaa ggcgttatgc tgcggcatcg
    23401 ggcgagtttc catcaggcgt ttgtaaccca ccatgccgat ggcaaaagtg atattggaga
    23461 gttgcaccag caacaagcca gtccagaaat ggtcggtgac ctgatcatag cgaataatcc
    23521 cggcaccaat caccgccaac aaggcactaa aagcatatcc ccagcgcaga cggcgcttac
    23581 tcatgatgtc ataaatcagc gtgatgtaga gcggcgtcag tacggtgaac agcagcagtt
    23641 cggaaaccgt caggtagaga taagcgcgga aactcagcat atacatcacg ccgagctgca
    23701 tcgcgcccac cagcatatac aggccgactg ttttcaggct attgccacgg gtacgcagaa
    23761 acggcagaaa aacgagtgcc gccaggccaa cgcgcaccag caccgcaaaa tagctatcga
    23821 cgtgccccgc aaggtactcg ccataaaagc taaaggagaa ggcccacaga atcgtggtga
    23881 tgataagtag cgccacaatg gaaaactctt tgattaacga gtatccattg tagcgaagta
    23941 tcaggttgac aactgaccaa ataaagaacg attacgataa atagagttta cgcagataat
    24001 gcggcaccgc gtcgtcggca ttagtaccaa tcacttccag ctcgggatga aggtctttca
    24061 gacgctggtg cgcactgccc ataatgcagc ctttccccgc catcgacagc atttcggcat
    24121 cgttcatccc gtcaccaaac gcaatacaat ccttcaggct gtagcccagt ttcttcgcca
    24181 ccgcttccag cgcatggcct tttgaaacgc cgcccgccat cacttccaga caggttaagg
    24241 tagagaaact aacgttgacg cgatcgcccc aacgagcgtt aatcgcctgc tccagcggca
    24301 gcagttgttc atgggaatcg caggtgaaaa acactttgct gacgccttcc ggctccagta
    24361 atccaggctc atacagcgca tatttgaaca ccgcttcttt aaaaaagcgc atctcttccg
    24421 ggcgatggcg attcataaac cattcgtcgt cgcgataaac gttagtaatg atgtccggat
    24481 tgtcgttgac tacgccaaac agatcgctgg caatgtcgcg atccaggtta tgagcaaaaa
    24541 tcagattgcc atccagatcg tgaacgcgcg cgccgttgga ggtaatcatg taagacttaa
    24601 tctccagatt atcgcgaatt tgccccacat caacgtggtg acgaccggtc gcaaacacaa
    24661 agttgatgcc gtgcgcggtg agcagcttca gggtttcttt ggcgtaaggg gataacgtat
    24721 ggtcgggaga aagtaacgtg ccatctaaat cagacgcaac aacctggtac ataagaaatt
    24781 taaacctctg tagagcggtt tccgctgggt gagttatgcc tgttgaaaaa atcaacaatg
    24841 gcgtggagcg cgactgagcg cattgcgtcc ttttcaaaaa ggatctcatg gtaagcacct
    24901 ttaattacca acggtcgtcc tccttcgaca ggatggcccg cggcggtgcg gagttcacaa
    24961 aaacggtcat gcatgcggtt atccaccacg cgttcctctt cagcctgcaa gagaagcgtt
    25021 ggcgtggcgt catcacccgc accggctaac acctgttcgc cagccagaat actttcgcgt
    25081 acccaatggt aggtcggccc accgacgcga atcgttgggt catcagcata gaagcgtaag
    25141 ttacgtcgat atcgctgtct gctgtgggtc agtacgttga tagcaaacgg caacgcgcgc
    25201 cagcgcccgg tgcctattgc atagccatca cggaaacgtg gatgcgcttc ggcccagttg
    25261 aggatctgcc gtgccataaa tgacggcata cgaatcacga tcccaaacat tggcgcagtt
    25321 agcgcaatgg cgtcacatac acctggatgg cgttgcagaa ataatgtgga gatcgcaccg
    25381 cccatcgaat gtgccagtat atagcgttta cgccacggac cgggctgaac ctcctgctgc
    25441 cagaatgccg ccagatcatc aacataatca ttaaagcgat taacatgccc gagatgcgga
    25501 tcggctaaca ggcgaccgga acgtccctgc ccgcgatggt cgatgattaa gacatcaaac
    25561 cccaaatgga acaggtcgta ggccagttcc gcatatttta cgtagctctc aatacgcccc
    25621 gggcagatga ctaccacccg gtcatggtgc tgtgcgcgaa aacggacaaa gcgcaccgga
    25681 atgtcatcca caccagtaaa ctctgcttca tcacgctgac gccagaaatc agtcagcggt
    25741 cccatggtaa aagcagcaaa cgcgttttct cttgtttccc agtctttttg ctgctgaaac
    25801 atcgggtaat ctgcctctta aaccacgtaa aatcgttttt tttagcgtgc ctgacacaac
    25861 gctgcgacag tagcgtattg tggcacaaaa atagacacac cgggagttca tcatgacctt
    25921 agaatggtgg tttgcctacc tgctgacatc gatcatttta agcctgtcgc caggctctgg
    25981 tgcaatcaac actatgacca cctcgctcaa ccacggttat cgcggcgcgg tggcgtctat
    26041 tgctgggctt cagaccggac tggcgattca tattgtgctg gttggcgtgg ggttggggac
    26101 gctattttcc cgctcagtga ttgcgtttga agtgttgaag tgggcaggcg cggcttactt
    26161 gatttggctg ggaatccagc agtggcgcgc cgctggtgca attgacctta aatcgctggc
    26221 ctctactcaa tcgcgtcgac atttgttcca gcgcgcagtt tttgtgaatc tcaccaatcc
    26281 caaaagtatt gtgtttctgg cggcgctatt tccgcaattc atcatgccac agcagccgca
    26341 actgatgcag tatatcgtgc tcggcgtcac cactattgta gtcgatatta ttgtgatgat
    26401 cggttacgcc acccttgctc aacggattgc tctatggatt aaaggaccaa agcagatgaa
    26461 ggcgctgaat aagattttcg gctcgttgtt tatgctggtg ggagcgctgt tagcatcggc
    26521 gaggcatgcg tgaaaaataa tgtcggatgc ggcgtaaacg ccttatccga cttactctga
    26581 agacgcgtct ggcatcaccg cgaaataatc aaatgaatgc caaatccggc aaataacgcc
    26641 ccggcaaaac catcaatcca cttcgccaga cgttgataac cacggcgcat ttgcggcagg
    26701 gcaaacaggc tggcaacgac ggtaaaccac gccagtgttt cgacaatgat cagcgcaaaa
    26761 atgccccagc gcgcggtagt gccaacgtta tcaccgacaa acaatgagaa caccgagcca
    26821 aagtagataa tcgctttcgg attagcgaga ttggtcagta aacctttcag gaaactgcgc
    26881 ccgcttttcg ccagctcaat ctgtggtgca ggcgcagata cagcctcttt tttcagtgca
    26941 ccgcgtagca tctggtaacc catccagcag agatacaggc caccgccaac cataatcagc
    27001 gtatgcagcc aggccatttt ttcgataatc aaatgcaggc caagcaacgc aatcccagcc
    27061 caaaccatta caccgcaggt aatgcccagc acgcccatca tcgcttcttt acgggaacga
    27121 ctgacagcgg tctgagagac aaaaaagaaa tcgggaccgg ggctcataag cgccacaata
    27181 tgcaccatgg cgacggtgag aaataacatc aacatacatt tgactcgcgg gggaaacgtt
    27241 tacggagtct tcatcctggc acttttttat gccgctgact actcttcgtc gtcgccgtca
    27301 acatgcgcgc ggataagcgc cataaacggt ttaccaaagc gttccagctt gcgcatccca
    27361 acgccgttaa cgctgagcat ttcgctggcg gtgatcggca tctgttcagc catctcaatc
    27421 aaggttgcgt cgttaaacac cacgtacggc gggacattgc tttcatcggc aatcgattta
    27481 cgcagtttgc gtaatttggc gaacagtttg cgatcatagt tgccgccgaa cgatttctgc
    27541 atcgctttcg gtttgagcgc cacgatacgc ggcacggcaa gttgcaaaga ggattcgccg
    27601 cgcagcaccg ggcgcgcggc ctctgtcagt tgtagggcag aatgctgggc aatattttgc
    27661 gtcaccaggc cgaggtgaat cagctggcgg atcacgctca cccaatgttc atggctttta
    27721 tcacggccca tgccatagac tttcagtttg tcatgaccat agtcgcggat acgctggtta
    27781 ttagcaccac gaatcacttc caccacataa cccatcccaa accgctgatt cacacgacca
    27841 atggtggaaa gggcaatctg agcatcggtt gaaccgtcgt actgtttcgg cggatcgagg
    27901 cagatatcgc agttcccgca cggctcctga cgcccttcgc caaaatagtt cagcaatacc
    27961 agacgacggc aagtttgcgc ttcggcaaac gcgcccatcg cattgagttt gtggcgctcg
    28021 atatcctgca actgcccctg cggcttctct tccagacaac ggcgcagcca cgccatatca
    28081 gccggatcgt aaaacagcat cgcttccgca ggcaggccat cacgcccggc gcgaccggtt
    28141 tcctgataat aggattcgat attgcgcgga atatcaaagt ggaccacgaa gcgaacgttt
    28201 ggtttattga tgcccatgcc gaacgccacc gtcgccacca caatttgcag gtcatcgcgc
    28261 tggaatttct cctgcacgtc ggcgcggaca ttattttcca gcccggcatg ataggccgcc
    28321 gcgctaatac ccttgctttg caggcgcgca gcggtgtctt ctactttcgc ccggctgttg
    28381 cagtagataa tgccggactt accgcgctgt tcctgcacgt agcgcatcaa ctgatcgagc
    28441 ggtttgaatt tctccatcag catgtagcga atattcggac ggtcaaaact gctgatttga
    28501 atcagcggat cgttcagccc cagcaggcgc acgatatcct ggcgcgtggt gtcgtcggct
    28561 gtggcggtca gcgccataaa cggcagcgtc gggaaccgct ggcgcaactg accgagcgcg
    28621 gcatattccg ggcggaaatc gtggccccat tgggagatac agtgcgcttc atcaacggct
    28681 aataacaccg gattccagtg cgccagatgc tcaagaaagt tatccagcat caggcgttcc
    28741 ggcgcgatat aaagcaggcg gatttgcccg gtgcgacagc ctgtcatcac ttcaagttgc
    28801 tgctcgcggg tttgcgtcga gttaaggcac gccgccgcca cgccgttggc ttgcagttga
    28861 tccacctgat ctttcatcaa cgaaatcagc ggtgaaacaa ccacggtaag gccgtttagc
    28921 aataaggcag gaatttgata gcaaagggat tttccgccac cggtgggcat gacgaccagg
    28981 caatcgcgac cggaaagcac agtgtcgata atttcttcct ggccggggcg aaactgttgg
    29041 tagccaaagg tttcttgtaa aacctgttta gctccggact ccagattcaa cacttccgcc
    29101 tgcgccacat tcaccccgat tgcctgaaat aaaaacaggc gctattttca gcgcctgcgc
    29161 gagaaactgc aatgctttta gtctctgaga gggtaatcct ggatgctgga ttccagattt
    29221 gccaaccact caaccgttca tcaaaacaaa tcgtttagca taacccccac accgacacgg
    29281 gtctggttga agttatagtc gatgagcgat tcgccatagc cgctgtaaac ctgagtataa
    29341 aggcgcacat gtttggtgat cgggtaactt aagcctaact ccgcgccgcc gtagccggtg
    29401 ttccagttgt actgtccttt cgcactgagc accgcatcac cgaggtgata gccgatttta
    29461 agctggtagt aacccatata tttggtgata tccgggttat cgtcagtatt acccaccaca
    29521 taccacggct tcacttctac cagccagtta ccgttttctg ccatcaggcg agtataaagg
    29581 cggttccagc tgcgggaggt cgggtctgaa cgcccgttag agtcgtggtt ataccccatc
    29641 tccacatcgc gcagcgtcca acctgcaaaa cggtaatcgg tggcaaaacc gaggaacaat
    29701 tgcggttcgt agttggtttc acgaaacggt gaagactctt cgctattgga cagttgccac
    29761 caggattttt gcgtataaga cgcacccaac accgagttcg ggcctaaaat cccacgccac
    29821 agtggaaatg ccaggctcaa ctgaaacttt acttcatcct tacgcgcatt ttccgcccag
    29881 tcgtaactgg caatcgcttc tttgttcaga tcgctggttt gggtgtagat gaggtagttg
    29941 gtgtcataag gatagagcgt gaacggattg tcatgctcct gcagcatatt ggcgataata
    30001 ctgccacgca ccgctggcgc gtcatgcacc tctttcaccg ttgcctcttg tgcatatact
    30061 gccataggca acataaacac cggcaacaac cagccctgca gagtccgcat cggtcgtgtt
    30121 ctccgtaacg aattattgat aaggtgaatg tttttctgcg ggctatttta catacttaac
    30181 tccattgccg ttagttatcc cttcctaaag tagaaaccaa caacaaagaa gcataaaatc
    30241 aacatttcat taaccaatga agtataagga tgatatgtct gccgtactga ccgctgaaca
    30301 agccctgaaa ttagtgggtg agatgtttgt ttatcacatg ccatttaacc gcgcgttagg
    30361 gatggagctg gagcgttacg aaaaagagtt cgcacagctg gcctttaaaa atcagccgat
    30421 gatggtgggt aactgggcgc aaagcatttt gcacggcgga gtcattgcgt cggcgctgga
    30481 tgtcgccgcc ggtctggtgt gcgtgggaag taccttaacc cgccacgaaa ccatcagtga
    30541 agatgaacta cgccagcggc tatcgcggat ggggaccatt gatcttcgcg ttgattatct
    30601 gcgcccaggc aggggcgagc gttttactgc taccagtagc ctgttgcgtg caggcaataa
    30661 agtcgccgtc gcccgcgttg aattacacaa tgaagaacag ctttatattg ccagtgccac
    30721 cgccacctat atggtaggtt gaagacataa aagcaggtaa actgctgtta ccaattaaaa
    30781 atgagtattc ccgatggatg caaaacaaac gcggcagggc gtattactcg ctcttgccgc
    30841 ttattttatt tggggtatag cgccagcgta cttcaagttg atttactacg tgcccgccga
    30901 tgaaatcctg acgcatcgcg tgatctggtc gtttttcttt atggtggtgc tgatgagcat
    30961 ttgccgccag tggtcctatt taaaaacgct gattcagacg ccacagaaaa tttttatgct
    31021 ggcagtctct gccgtgctga ttggtggcaa ctggctactg tttatctggg cggtgaacaa
    31081 tcaccatatg ctggaagcga gccttggtta ctttattaac ccgctggtga acattgtgct
    31141 ggggatgatt ttcctcggcg agcgattccg ccggatgcaa tggctggcgg tgattctggc
    31201 gatatgtggc gtgttagtcc agctgtggac ttttggttcg ctacctatta tcgcgctggg
    31261 gctggcattt agttttgcct tctacggcct ggtgcgcaag aagattgcgg tagaagcaca
    31321 aaccggcatg ttaattgaaa ccatgtggct gctgcccgtg gcggcgattt acctgtttgc
    31381 tattgccgac agctcaacca gccatatggg gcaaaacccg atgtcgctga atttactgct
    31441 gatcgccgcc ggtattgtca ctaccgtacc gctgttgtgt tttactgccg ctgccacgcg
    31501 cctgcgtctc tcaacgttag gctttttcca gtacattggc ccgacgctga tgttcctgct
    31561 ggctgtgacg ttttatggtg aaaaaccggg tgccgataag atggtgactt tcgcctttat
    31621 ttgggtggcg ctggcaattt ttgtgatgga tgcgatttat acgcagcgta gaacgtccaa
    31681 ataaaaaata aatatattct gaaaaaatat gccgactctt gtatgagagt gggcatattg
    31741 ccatgctgac aggagactca cacaaggatg taaaatttat gttacgtatg tttatcccaa
    31801 catctaacgg taagatttcc agacgccgat atatattttc atttatttta ataaatttca
    31861 tttttgcctt tcttattatc ttttttaacg atggggaagc aggttttctt gttatagtct
    31921 ctaccatcgt actccattat ctcgtcatca acatgaattg ccagcgactc cgtgatagtg
    31981 gtttcatcta tataaaaact tatgtttttg gtacattagc agtatatatc atctctatca
    32041 tcacaatgat agccgaagat tttgcctgtt caggcaatgg ttcgatgatt tttttgattt
    32101 gttatttttc cactttcagt atgctaatgc ttgcacctac tgattcatca aaacaatagt
    32161 tatggagtat ttatgagtaa ggaatatatg aacgatggtt cattaagtga aaaatggaaa
    32221 taccgattta atttttatga tcaacatggc tttcctggat tctggggagc aacaccagaa
    32281 tataaagcag catttaaagc attaaaagta agacaacgat taactattca aatgaatttc
    32341 attgctttct tctgttcctg gatctattta tttgttctgg gcttatggaa aaaagcaatt
    32401 atagttctat tacttggcat tctatctcta tttgttggtg cattaattgg cgttaacatt
    32461 ctgggaattg ccgtagcagc ctatgttgct gttaacacga acaaatggtt ctatgagaaa
    32521 gaggtaaaag ggttaaatac ctggagcctt tgaataacag gtggcttagc cagactaagc
    32581 caccgctctc gttttttaca accagttctt ccgcttaaag tacagatacg gtgccaggcc
    32641 cgcgaggatc ataaagataa tcgcgccagg gtagccgaaa ctccacttca gttctggcat
    32701 aaactcaaag ttcatcccat aactggaagc aaccagcgtt ggcggcagga acacgacgga
    32761 taccaccgag aaaattttga tgatgcggtt ctgctcgatg ttgataaaac ccattgccgc
    32821 ctgcatcagg aagttcacct tctggaacag ggattcgtta tgcggcagca gggattcgat
    32881 atctcgcagg atttcacgcg cctgctccag ttgcccaccc ggtaaacgcg ctttacgcac
    32941 caggaagttg agcgcacgct gggtatccat cagacacagg cgcactttcc agccgatatc
    33001 ttccagttcc gccagagtgg agagcgcctc gtcgtactca tcgccctgat gcccttccat
    33061 aatcacccgg ctcaactgct ccaggtcgct atagatattt tcaatttcat ctgccaactg
    33121 ttcgattttg gtttcgaaca gatccagcag cagctcgtag gcgttaccgt ctaccatcga
    33181 ctggctacgg gcacgcatac gatacagacg aaaagcgggc agttcacgct cacgcagagt
    33241 aaacagacga ccatcacgga tggtaaatgc cacagtggag ttaccggcgt gatcttccgc
    33301 atcttcaaag aagaagaagg agtgaatatg caggccgtcg tcgtcttcaa agaaacgtgc
    33361 cgatgcttcg atgtcttcca gttccgggcg ggttgccagg ctctggccaa gttcagattg
    33421 tacgcgcagt cgctcgtcgt cgtccggttc gacaagatca atccatactg catttacaag
    33481 gggttgtgac tcttcgactt ccagccgggt cagtcggtta ttttccagtt gaaatgcgct
    33541 cagcatgacc gggactccca atgcttaaaa tatcggacag ttcggtgggc acacagaaac
    33601 aaattgggtt tcagaccatt aaacagcctg actcagcgcg acgggaaaaa ttgaggtcgc
    33661 tgacaaccgc taaggctatc agcaaaaagg gatagcctta ggagttgatc ctggatgaca
    33721 ggatagtgag ccagtatcta ctgggtgtgt ccaaggcgaa tgtcctctta gagtgatcgt
    33781 gcgcgcatgt tacgccagca aaattttgcc gtcaacacgc aacagaacac cacagaacaa
    33841 tagttgaact tcgcgctttt tggtactcgt caaggtcgct aacaggataa aaaaatccca
    33901 catccaggac ctataatggc gcatcggcta ctcattggta aaggaatgat caccttgaat
    33961 ctcaaacgga tttttctcgc cctcacctta cttcccttgt ttgctgttgc cgctgatgat
    34021 tgcgcactct cagatccgat gctgatcgta caggcgtata ccgttaatcc tcaaacagag
    34081 cgggtgaaaa tgtactggca aaaagccaat ggcgaagcgt ggggaacttt acatgctctg
    34141 ctggtggata tgaatagtca gggtcaggtg cagatggcga tgaacggcgg catctatgat
    34201 gaaagctatg cgccgctcgg tttgtacatc gaaaacggtc agcagaaggt ggcgttaaat
    34261 ctcgcttcag gtgaagggaa tttctttatc cgtcctggcg gcgtgtttta tgtcgcggga
    34321 gataaagtcg gcatcgttcg tctggatgcc tttaaagcca gtaaagagat tcagtttgcg
    34381 gtgcagtcag ggccaatgtt gatggaaaac ggtgtaatta atccgcgtat tcatcccaac
    34441 gtcgcctcac gtaaaattcg taacggtgtt gggattaata aacatgggaa tgccgtgttt
    34501 ttgttgagcc agcaggcaac aaatttttat gatttttcct gttatgccaa agcgaaactg
    34561 aacgttgagc agctgcttta tcttgatggc acgatttcac atatgtatat gaaaggcggg
    34621 gcaatcccgt ggcaacgcta tccttttgtc accatgattt ccgtcgagcg gaaaggttag
    34681 aatgatagtt ttgtgtcaga tactaaatat gctgctgatg ccggatgcgg cgtaaacgcc
    34741 ttatccggcc tacatgacgt tgcaatttat tgaatctgaa tgatttttta ggccaaataa
    34801 ggtgcgcagc accgcatccg gcaacgttac accgtctcca gccgggcgta agccgccacc
    34861 agccatttga ttccctggcc ctggaacgcc acctgcaaac ggctatgttc accgctgcct
    34921 tccatattga cgatggtgcc ttcaccaaac ttagcgtggc gtacacgctg accgagcttg
    34981 tagccgctgt cgttctcgac catcggcgtc cccatccgtt gatggctgac cggacgactt
    35041 accgtagcgc gcaggcgcac ctcttccaca cactcttccg gcagctcgcc gataaagcgc
    35101 gacgggcgat ggtaaacctc tttgccatac agacggcgag tttccgcgta ggtcaacgtc
    35161 agtttctgca tcgcgcgggt tacgccaacg taggccagac ggcgttcttc ttccagacgc
    35221 ccgccttcat ccagcgacat ctggcttggg aacatgccct cttccatacc gacaataaac
    35281 acctgcggaa actccagacc tttcgccgag tgtagcgtca tcaactgcac cgcgtcctgc
    35341 caggtatccg cctgcccttc gcccgcttcc agcgccgcat gggagagaaa cgcctgcaac
    35401 ggcattaaat cttcatcttc ttcgttgtag ctgaactggc gcgttgccgt caccagttcc
    35461 tccaagtttt cgatacgcgt ctgacctttt tcgcccttct cctgctcgta catggtgcgc
    35521 aggccggagt ctttaatgac ccggtcagtc tgtacatgta gcggcatatc ggcagtttct
    35581 tgcgctaagg cgtcgatcag ttccataaac cgctgcaagg cgctggctgc acgtccggcg
    35641 agcgcttttt cctgcaacag ttcacggcac gcctgccaca gcgttaactg gcgatcgcgc
    35701 gatgtctgac gtaccacgtc cagcgtccgg tcaccaatac cccgcgttgg tgtattcact
    35761 acgcgctcaa aggccgcgtc gtcgttgcgg ttggcaatca ggcgcagata cgagagcgca
    35821 tctttgattt cctggcgttc gaagaagcgc atcccgccgt aaatacggta cggcatactc
    35881 gcctgcaaca gtgcttcttc cagcacgcgc gactgggcgt tactgcggta gagaatggcg
    35941 cactcggcaa gcgctccgcc gttatcctgc caggttttga tgcggttaac cacaaaacgc
    36001 gcttcgtcga gttcgttaaa ggcgcaataa agagaaatcg gctcaccgtc cgcgccatcg
    36061 gtccacaatt ttttacctaa gcgcccattg ttattttcaa tcagggcgtt agccgcgctc
    36121 agaatattgc tggtagagcg gtagttttgc tccagacgaa tagtttcggc accggggaaa
    36181 tcattaagga aacgctgaat attctctacc tgcgccccgc gccagccgta gattgactgg
    36241 tcgtcatcgc caacgatcat cactttgccg gtatcgcccg ccagcaggcg gatccacgcg
    36301 tactgaatgt tgttggtatc ctggaattcg tccaccagga tgttggtaaa acgttcgcgg
    36361 tagtgttgca ggatatgcgg cttgttaagc cacaactcgt gagcgcgcag cagcagctcg
    36421 gcgaagtcca ccaggcccgc acgatcgcac gcttcctgat acgcctgata caccttctgc
    36481 caggtctgct ccaccggatt accgtagctt tgaatatgat gcggacgcag gccttcatct
    36541 ttctggctgt tgatgtacca cattgcctgg cgcggcggcc actgcttctc gtcgaggttc
    36601 atggctttga tcagacgctt aagcaggcgt agctggtctt cactgtcgag gatctggaaa
    36661 tcctgcggta gattggcgtc catatggtgc gcacgcagca aacggtgcgc cagcccgtgg
    36721 aaggtgccga cccacatgcc gccctggctg gtgcccatca gttgcccgat acgatgacgc
    36781 atttccgccg ccgctttgtt ggtaaacgtc accgccataa tcgagtatgg cgagcagttt
    36841 tccacgctca tcaaccaggc gatacgatgc accagtacgc gcgtcttacc actgcccgcg
    36901 cccgccagca ccagaaggtt gctgcgtggc gcggccaccg cttcgcgctg tttgtcatta
    36961 aggctgtcga gcaggtaaga aacgtccatt ggcaccgccg cgtaaaaata ggttgggcgg
    37021 agaagcgcaa cgccctccgc caaaaagctg ggtatatata cagatttgct gattatatca
    37081 gcgaggtcag agatgccaac cgggaaattt ccagatgcgg cagtaaacgg ctgtcccagg
    37141 tttgcatcag atcgccattt tccggtctga tccaacaagc ctgcattccg ctgcgaattg
    37201 ccccacccac gtcagtggtg agatcgtccc caacatgtaa gatctcgccg atcggcacgt
    37261 tgagtttttc cgcagccaaa aagtacatat cgctgaacgg ttttgagcgc ccgtgcgggc
    37321 cagcgcgcag cacaaactca aaataatccc ccaaaccaaa cagctccggc tgggcgttac
    37381 cgttggtgat cgccaccagc ggccatttct tcgccagctg ttttaaggtg tcgtgagttt
    37441 gctgcgggac gtcgattcgg ctgcgccatt tggcaaagtt gatcattgct gcgtgtgcgc
    37501 ctgcactggc ttcttcggca ctcagcccgg cgtcgagcat cgcttgttca atcgaacgaa
    37561 aacgccagcg cgtcacgtcg tgataaatct cgggttccgc ttcccgtacc gcctggcgca
    37621 ggcgttgcag atcttcattc tggaagctgc gcagcgccgg atgataattt tgcacaaagg
    37681 taagcgcctc tcgctcggtg cgcaaaatca ccggacggtt atcgtaaagg gtatcatcca
    37741 ggtcaaaggt gagcgccgag atgcgcccca aaggccggta aaaacgcatt atttcccccg
    37801 tttggcgcgt ggatgcgccg catcgtacac cgaggcaagg tgttgaaaat caagatgagt
    37861 atagatttgc gtggtggaga ggttggcatg acccagcagc tcctgcacac cacgaagatc
    37921 gccgctcgac tccagcatat gcgtagcgaa cgagtgacgt aatttatgcg ggtgaacgtg
    37981 attattcagc ccttgtttta tgccccattc ggcaaagcgt ttctgcacat tacgcgcgga
    38041 gatacgcttg cccagtttcg acagaaaaag cgcgtcgtct tcgctaccaa acaggtcgcg
    38101 caaatcaagc cagtgctcaa tccacgccac agcgttgcga ccaatcggca ggcggcgctc
    38161 tttgctgcct ttccccatta cccacacttc accagactcc aggtcgaggt gtttgatatc
    38221 cagccccacc agctcggaaa gacgcagacc cgcgccgtac atcacttcca gcattgcacg
    38281 gtcgcgtaca gcgaggggat cgttgatatc aatatccagc agccgattca tatcgtcgac
    38341 gtcgatgttt ttcggcagat gacgcggcgc tttcggtgcc gaaactcctt tcgccgggtt
    38401 agctttgagt tcgttctggc tgaccagcca gtcaaaaaag ctacgtagcg cagaaagccg
    38461 taacgccaga cttgctgctc ccagcccttt acggcgactg cgtacagcaa aattgcgtac
    38521 catcgctgca tcgcattgtt gccagctttg caggccgttt tcgctggcaa aattgatgat
    38581 cgcctcaagc tgacgctggt agttaagtag ggttatcggg ctaagctgac gctccacgct
    38641 cagataacgt aggtagcgtt ctacatcggt gtgtaaatcg gtcatacgcg ttcaatccaa
    38701 cgctccagaa gctccggcaa catcagcgca atttcatgaa gtaactgcgt tccctgccct
    38761 tgttgatagt gactggcatc acgactggta aacagcacga cacccaaatc agcatcgctt
    38821 cccagcatcg acatcgccac cgatcccacc gctttcgctt ccggtagcac caccagcagc
    38881 tctggtccgt taagcggccc aagatagtgc tgttcctgcc ccaaacgctg aatacgcagc
    38941 ggttcgaaag actgacggct taatgccaga tgagtgtggt tcgacggcgc acctaagcgc
    39001 cagcgatccg gaaacaggcg cagactcgca cctgccaggc cgagatcgcg cgcccagcgg
    39061 tgaaagcgca tcagcatatc gtcgagactg ctggcggcgg tgagactgcg ctgcaggtag
    39121 agtaggcgat aaaacaggcc ttcgttggcg atagcctgtt ccatcaacag caccatgttc
    39181 tcttccagaa catgaatatg attacgtgcg cgggccatgt gccactcgac caacgaaacg
    39241 gtgccgcgta ccggatgcgg cacacgtatc gcttctactg cgcgcgcatt gcggataaaa
    39301 aactcaggat ttttaatcag ataatcgaca accgcccggt catcaagctc cgtgagtgtt
    39361 tcctgcagtt cttcccctgg ttgcttcata gatgaataaa tccgtcgtag acatgtaccg
    39421 ccgggccagt catatataac gggtgacccg gacctttcca ggcgatatca agacgaccgc
    39481 cggggagttc cacgcgtact tcttcggcca gcaaaccttg ctgaatccct actgcaaccg
    39541 ccgcacacgc gccgctgccg caggcctggg tttctcctgc cccacgctca taaacgcgta
    39601 aacgaatatg ctcgcgctta accacttgca taaaaccgat attggctcgc tccggaaaac
    39661 gctcgtggct ttccagaaca ggaccaagcg tttctaccgc cgcggtatcg acatcatcga
    39721 cctgaatcac gcaatgcgga tttcccatcg acaccacgcc gcataagatt gtctgctcgg
    39781 cggcgcgcat aatataggtc ttttccgctt tgttagcgcg aaacggcaca gcggaaggtt
    39841 cgaagttggg ttcgcccata tttacgcgga ccaaatcatc atcggtgacg gtcagaacca
    39901 tccgcccgtt ggcggtgctg acgcggatat cgcgcttatt ggtaagccct ttcaggcgca
    39961 caaaacgggc aaagcagcgc gcaccgttgc cgcactgcgc cacttcactg ccatcagcat
    40021 tgaaaatgcg atagtgaaaa tccagttcag gatcatacgg cggctcaacc accagcagct
    40081 ggtcaaaccc tacccccagg tgccgatccg ccaggcgacg aatcagctcc ggtgaaaaaa
    40141 agacattctg cgttaccgcg tcgacgacca taaaatcgtt gccaaggcca tgcattttcg
    40201 agaactgcat catttactcc aatcacgcgg gtacagaaac tgacttttag taattcacct
    40261 gggatggacc atcgccagtg gcgcgatcgt ttttatccgg caccgtggat tgcgtttgcg
    40321 tctctaccgg tttggtcggc ggcggtgcat ttttatctgc aggcgggaaa tagagcggac
    40381 ctttcagacc gcagcccgtc aggctgaaga gagtaagtaa tacagtgagt gccttaaaca
    40441 cgtttttcat tatggattgc ctgtaaagtt catcgctttc tgctttctat catcgcagga
    40501 gaaggcgtaa aagcaagcat ttagcgactc tttatgaagt ctgaaaagtg aggaaatgct
    40561 gcgagtataa cgcttactcg tggggattta tgttctgctg gcagtttcgt gatcaacacc
    40621 tcgcgccagg atggctttat gcactatcgc aatgtggttt gtttgttggc ttttttagtt
    40681 ccgtcagcgt gaggatgctc gctcggtaaa cctgatcata atgtttacca gaaacaggga
    40741 aaggcgtggt gtaactgcgc cctaatgaag agacaaatct ttcgctacac tgagacatca
    40801 ttattccggt gacggatgaa tcgtgccgga acgcagccgc agaggtgcaa actttgaagc
    40861 cctgacccca tgccgctata ctgccgccat cacttaaaaa caggatacaa caatgaacga
    40921 cagtgaattt catcgcctgg ctgatcaact ctggctgacc attgaagaac gcctggacga
    40981 ctgggatggc gacagcgata tcgactgcga aatcaacggc ggcgtactga ccattacctt
    41041 tgaaaacggc agcaaaatca ttatcaaccg acaggaaccg ctgcaccagg tatggctggc
    41101 aaccaaacaa ggcggctacc attttgatct gaaaggcgat gagtggattt gcgatcgcag
    41161 cggcgaaacc ttctgggatt tgctggaaca ggcggcgacg cagcaggcgg gtgaaacagt
    41221 cagtttccgc taagattgca tgccggataa gcctcgcttt ccggcacgtt catcacgaaa
    41281 aatactgctg taatagcggc gtatcgtgat cctgattggc aggcggcatg ttaccgatag
    41341 attttgtgcg gaacggaatc acctgttcac gaccatcaac cttcacaatc tgatagaact
    41401 gcggcaggtt gaagttgatg aagcttgagc cgtaggtaaa acggtcatgc gatgacgagt
    41461 agaagcgact gacgtcacgt accagctcct ctttgctgcc ttcgcagtgg tgatacacct
    41521 caacccggtt gctttcgtcg agaatgtaga tattaaagcc attctcgtct tgcgtttctt
    41581 cgaaaaagaa ctggatgatc ccttcgctgg caaagccgtc caccaccgcc ggtaatttga
    41641 cgtgattggt ttcaacctgc actgacaggc cgtgcagttt gttatgcgaa atcgcgccat
    41701 aaaactcgat ggcgttttcc agtttctgta ccgatacatt caggcgttcg aagaacaacc
    41761 cccaggtttg accagaaacg cgcagcgcct tgaaacgccc ggtttcctgg cgggtgctgg
    41821 aaagacgcaa ttcaatacac tcagaaacca gttgctgcac gcgagtacga attaagccgc
    41881 gcagatgctg gctataacag aagacttcca cgctatctgg cggtgcggcg tcctgatgca
    41941 ttttgccgag aatagttttc agggcttcga tcatcgattg ctcgccgttg aaatgcagcg
    42001 tacgcacttc gttccacgag ttgcggtaca gcaggtcaac gctacctacc aggcaatttt
    42061 gattctcgcc aaagctgaag acatccagct tacggaaatc gaaatgcacc acctgattgc
    42121 ggaacgccgc tgtcgggtca tattccaggt taacgataat cgccagatgg cggatctcac
    42181 acgggctgta gagcgccttc ggtgtcggtg caggtaagcg cagcgggaaa tggtgcgaca
    42241 cgtcggcgac catctcctgc aacttaggca aatcgacaat gccgttacct ttaatataca
    42301 aacgggtgcg cgaggtcagc aggccgttaa accatgccca cgccaccagt ttattcaggt
    42361 aacggttata ttccagcggc tgatggctga tgatcgactc aatatttggc gcgcggttat
    42421 acagatacca acctgaacgg ttagcacggc ccggcggcac ataaataaag gtcagattcg
    42481 gttccgagag atcgggtgaa atctgcgggt ttaccagcgt cactttacct ggtaatgctt
    42541 caaacgcggc atacagctta cgcgtcagca cgccgatatc ctgcggactg gcggagacgc
    42601 taaggttatt gcgacgcgca aagcggatca gattacggta gctctgcatc atcgcgtcga
    42661 gcaactcgtt gtgcgcctca cgcacctgat caatcttcca gttagcgcgg ttatcgagca
    42721 ttgccagacg agcttcgtcc caaccccact cgctcactaa ctggctcaac actgcgcgac
    42781 gccagcctac gcaggcgcgt tcacggctga gcttttcgca cacttttaaa tagaagcagc
    42841 gacgtactaa atccagacgg gtaaaatctt caatcgccgt caggtattca gtaacacgct
    42901 ccagcatcat gcagtatgga tcgagaccaa acgatacaat ctcgccgtcg tgcaaacgct
    42961 gtttgatatc tttcgccagc agacgtgggt tcgggtattc ccaggaatag gcttccagca
    43021 gcagtgtttt cagcaccgct ttgtatgggg aatcgatact cttgtagagc tgccaaaggc
    43081 tggcaccaaa gtactcttca gcagaaagcg agcttaagcc accgagatcc agccattcat
    43141 ttggcgtcag cacgccctgc gcgtaaagcg tcatcacata gtcgtcgtaa tgttcttctt
    43201 cgtcgcacgg caccatattc cacagaatac gcttaccggc gagacgcacg gcggtacgat
    43261 aaaattcgtc aagcagcagt atatgctggg tggagccaca atcttcgccc cccaggctgc
    43321 cgctttcatt atgacggaag cggttttcat caatcaggaa gaagctgact tccacaccca
    43381 gcgaggcggc ccagctttcc agcagactac atttacgttg tagcaactgg cgctcttcgc
    43441 tatcgagcca ggattgatga cagacccaga tatccaggtc agaggaacaa ctttgcccta
    43501 cggacgaggt gctgcccatg gtgtatacac cagtaattgg aagctcacct ttcggcgggt
    43561 cctgtactga cattccacga tacagttcaa gctcgttcag gtagtggcgt tgagtttcat
    43621 caggcgtgta aaggcaaatg cctttgggaa cgttaccatc aaggtaaccc ggcattagcg
    43681 gatggtgata gtgcaacaat gtcggcagta gactgtagac ctgttggaat gcaggcccca
    43741 tagcagcaag cgcgcgatcc acacgcaatt gatttatggc atccagtctc tgtttcagag
    43801 tctcaatata gaggtacaag acgtatcgcc tgatttgcta cccgtcatga ctgtgattcc
    43861 gccaacatca acggtaacac gcggcattcg ggatatttcg tatgtcaaag gtaaccgtta
    43921 ccacttttcg cgcctggttt ttttagtttc acgacgaaaa aatggtctaa aacgtgatca
    43981 atttaacacc ttgctgattg accgtaaaga aagatgcgct acatacaagt gtagcaccgt
    44041 ttattctctg taaattcctt attacgacgg cgtgaaacgc ctgtcaggat ccactgccag
    44101 acctcatttt acggtttgcg caggcgtcta cgtttcacca caacactgac atcactctgg
    44161 caaggatgtt aggatgaacc acggatgata atgacggtaa caagcatgtt agacaatgtt
    44221 ttaagaattg ccacacgcca aagcccactt gcactctggc aggcacacta tgtcaaagac
    44281 aagttgatgg cgagccatcc gggcctggtc gttgaactgg taccgatggt gacgcgcggc
    44341 gatgtgattc ttgatacgcc gctggcgaaa gtaggtggaa aaggcttatt tgtaaaagag
    44401 ctggaagtcg cgctcctcga aaatcgcgcc gatatcgccg tacactcaat gaaagatgtg
    44461 ccggttgaat ttccgcaagg tctgggactg gtcactattt gtgagcgtga agatcctcgc
    44521 gatgcctttg tgtccaataa ctatgacagt ctggatgcgt taccggcagg cagtatcgtc
    44581 gggacgtcca gtttacgtcg ccagtgccaa ctggctgaac gccgcccgga tctgattatc
    44641 cgctccctgc gcggcaacgt cggcactcgc ctgagtaaac tggataacgg cgaatacgat
    44701 gccatcattc ttgccgtagc cggactaaaa cgtttaggtc tggagtcccg cattcgcgcc
    44761 gcgttaccgc ccgagatttc tcttccggcg gtagggcaag gtgcggtggg tattgaatgc
    44821 cgccttgatg atgcacgcac tcgcgagctg cttgccgcgc tgaatcacca cgaaactgca
    44881 ctgcgcgtta ccgcagaacg cgccatgaat acccgtctcg aaggcggatg tcaggtgcca
    44941 attggtagct acgccgagct tattgatggc gaaatctggc tacgtgcgct ggtcggcgcg
    45001 ccggacggtt cgcagattat tcgcggtgaa cgccgcggtg cgccgcaaga tgccgaacaa
    45061 atggggattt cgctggcaga agagctactg aataacggcg cgcgcgagat cctcgctgaa
    45121 gtctataacg gagacgcccc ggcatgagta tcctggtcac ccgcccgtct cccgctggag
    45181 aagagttagt gagccgtctg cgcacactgg gacaggtggc ctggcatttt ccgctgattg
    45241 agttttctcc gggtcgacaa ttaccgcaac ttgctgatca actggcagcg ctgggggaga
    45301 gcgatctgtt gtttgccctc tcgcaacacg cggttgcttt tgcccaatca cagctacatc
    45361 agcaagatcg taaatggccc cgactaccta attatttcgc cattggacgc accaccgcac
    45421 tggcactaca taccgtaagc ggacagaaga ttctctaccc gcaggatcgg gaaatcagcg
    45481 aagtcttgct acaattacct gaattacaaa atattgcggg caaacgtgcg ctgatattac
    45541 gtggcaatgg cggtcgtgag ctaattgggg ataccctgac ggcgcgcggt gctgaggtca
    45601 ctttttgtga atgttatcaa cgatgcgcaa tccattacga tggtgcagaa gaagcgatgc
    45661 gctggcaatc ccgcgaggtg acgacggtcg ttgttaccag cggtgaaatg ttgcagcaac
    45721 tctggtcgct gatcccacaa tggtatcgtg agcactggtt actacactgt cgactattgg
    45781 tcgtcagtga gcgtttggcg aaactcgccc gggaactggg ctggcaagac attaaggtcg
    45841 ccgataacgc tgacaacgat gcgcttttac gggcattaca ataactctca taacaggaag
    45901 ccataatgac ggaacaagaa aaaacctccg ccgtggttga agagaccagg gaggccgtgg
    45961 acaccacgtc acaacctgtc gcaacagaaa aaaagagtaa gaacaatacc gcattgattc
    46021 tcagcgcggt ggctatcgct attgctctgg cggcgggcgt cggtttgtat ggctggggta
    46081 aacaacaggc cgtcaatcag accgccacca gcgatgccct ggctaaccaa ctgacggcat
    46141 tgcaaaaagc ccaggagagc caaaaagccg agctggaagg cattattaag caacaagctg
    46201 cacagcttaa gcaggcgaat cgtcagcaag aaacgctggc aaaacagttg gatgaagtcc
    46261 aacaaaaggt cgccaccatt tccggcagcg atgctaaaac ctggctgctg gctcaggccg
    46321 attttctggt gaaactcgcc ggacggaagc tgtggagcga tcaagacgtc acgaccgctg
    46381 cagcgttgct gaaaagtgca gatgccagcc tggcggatat gaatgacccg agtctgatta
    46441 ccgttcgccg ggcaattacc gatgatatcg ccagcctttc tgcggtatcg caggtggatt
    46501 atgacggcat catccttaag ctcaatcagc tttcaaatca ggtagataac ctgcgtctgg
    46561 ccgataatga cagcgatggt tcgccgatgg attccgacgg tgaagagctt tccagttcca
    46621 tcagcgaatg gcgtatcaat ctgcaaaaaa gctggcagaa ttttatggac aacttcatta
    46681 cgattcgccg tcgtgatgac accgccgtac cgctgttagc gccaaatcag gatatctatc
    46741 tgcgcgaaaa tattcgctct cgcctgctgg tcgcagcaca agctgtaccg cgtcaccagc
    46801 aagagactta tcgccaggcg ctggagaacg tctccacctg ggtacgtgct tactacgata
    46861 ctgatgatgc caccaccaaa gcgttcctcg acgaggtgga ccagttaagc cagcaaaata
    46921 tctcgatgga tcttccggaa accctgcaaa gccaggcgat gctggaaaaa ctgatgcaga
    46981 ctcgcgtgcg taacctgctg gcacaaccgg cagcggggac aacggaagct aaacctgcac
    47041 ctgcacctgc acctgcaccg caagctgata cttcggcagc tgcgccgcaa ggagaataac
    47101 catgctaaaa gtgttattgc tctttgtgtt gctgattgcg gggatcgtgg ttggcccgat
    47161 gattgccggt catcagggtt atgtgctgat ccagaccgac aactacaata tcgaaaccag
    47221 cgtcacgggc ctggcgatta ttttgattct ggcgatggta gtgctgtttg ccattgagtg
    47281 gctactgcgg cggatcttcc gcactggcgc gcacacccgt gggtggtttg tcggacgtaa
    47341 gcgtcgccgt gcccgtaagc agaccgaaca ggcgctgctg aaactggcgg aaggcgatta
    47401 tcagcaagtt gaaaagctga tggcgaaaaa tgccgatcac gcggaacaac cggtggtgaa
    47461 ctatctactg gctgccgaag ccgcgcaaca acgtggtgat gaagcacgcg ccaaccaaca
    47521 tctggaacgc gcagcggagc tggccggcaa tgacaccatt ccggtagaaa tcacccgtgt
    47581 acgtctgcaa ctggcccgta atgaaaacca tgctgcacgc cacggcgtgg ataagctgct
    47641 ggaagttacg ccacgccatc cggaagtatt acgtctggcg gaacaggcgt atatccgcac
    47701 aggtgcatgg agttcgctgc tggatattat cccatcaatg gcgaaagccc atgttggtga
    47761 tgaagaacat cgcgcaatgc tggaacaaca ggcatggatt ggcctgatgg atcaggctcg
    47821 tgccgataac ggtagcgaag gtttgcgtaa ctggtggaaa aaccaaagcc ggaaaacgcg
    47881 tcatcaggta gcgttgcagg tggcaatggc ggaacatctt attgaatgtg acgatcatga
    47941 tactgcccag caaattatca tcgacggcct gaaacgccag tatgacgatc gcctactgct
    48001 gccgatcccg cgtctgaaaa ccaataaccc ggaacagctg gaaaaagtgc tacgccagca
    48061 gatcaaaaac gtcggcgatc gcccgctgtt gtggagcaca ctgggccagt cactgatgaa
    48121 gcacggagaa tggcaggaag catcgctcgc cttccgcgcg gcgctgaaac aacgtccgga
    48181 cgcctacgat tacgcatggc ttgccgacgc gctggacaga ctgcacaagc cggaagaagc
    48241 tgcagcgatg cgtcgcgacg gtttgatgtt aacgttgcag aacaacccac cacagtagtt
    48301 ccttctcacc cggaggcaag cacctccggg acctttctga tgcataaaaa aacgcctgct
    48361 cttattacgg agcaggcgtt aaaacaggtc tgtatgacaa caagtgggtg cttcactcaa
    48421 cgttgtgtcc atggtgtctg atgaggcata agcgacatct gtcagtggac gataagcacc
    48481 gtaaacggct ctgcgtcatt ccggagttta tgaggcacta aggcgaacat aagagatgga
    48541 atgagcatct acccgtttat tatgccacag agaatcggga aataacatcc cttaacactt
    48601 gttatgagat aattctgtaa tcctctttgc ttcctgagta ataacttcct gagtgaatat
    48661 ttaacctgag cttgatcctg cacatactta ttatgaatga taaaattcat tcaattaata
    48721 acacatatat taattgccgt taaaactaaa aaaagcatca ataatcaacg cgatataata
    48781 aacctgcctt acatatcaac tgcgccagag gtaggattga aaacgctctc ctgattttcc
    48841 aattcatttt ctggataaat aaataattta tttttgtcac tattatttat gtaatcatcc
    48901 tgtcagggag agggatctca attatcaatg cttaattacg tcatcatttt gatgacatga
    48961 aaccggagca ttttgcatgg aattttcgtt ttcacccaaa cttcttgttg ttgctgtcgc
    49021 cgccgctctt cctctcatgg ccagcgcagc agataccccg tcaactgcca ccgcacgcaa
    49081 aggctttgcc ggatacgatc acccaaacca gtatccacag aacgctgcaa tttattgaat
    49141 ttgcacgatc atgtaggccg gataaggcgt ttacgccgca tccggcaatc aaccgcaggc
    49201 ggccacaaaa tggccgcttt tttacttact cactaccagc aaatgtgcct gcataatgtc
    49261 actggccggg cgaccgtgcg ccagcaaatc agccattgct ttaagatatg gcggtagatg
    49321 gcggaaataa cgctgatacc cggcacacaa ataattcagc cccggtttgc cgctggcatc
    49381 gagcatgaag cggtgtttcg ggcagcctcc ccagcacgct tttaacacgt tacaactgcg
    49441 acactgcgcc ggtaattgct taaatttatc ttcaccaaac acctgctgtt gcggggaatc
    49501 gatcatttct gcaattgttt gctgatgcat attccccagc cgatattgcg gataaacata
    49561 gtgatcgcag gcgtaaacgt cgccgttgtg ctcaacaatc accgagcgcc cacaggttgg
    49621 ctgatgatgg caaaccgcac ccggcgcacc gacaaaattg gcaaacgccc attcgatatt
    49681 catcacgaaa atcttgccga cgtcgcgttt gatccagtgg tcgaatatcg ccaccagaaa
    49741 ttcaccaaat ttatcggggc gcaccgacca ttccgtcagt tccccctgaa tatcaccagg
    49801 cgcatgcaat ttcagtccat cgcgggcagc tgtttcatca gccagacgct cgaccaccgg
    49861 aataaactgg atgaattcga ctcccgcatc gcacaaaaaa tcataaacct gcaacggttg
    49921 ctgcgcgctg gtgcggttga cgcagaccag cacgttatag tcgacatgat gtttttgcag
    49981 cagcgtcagg gcacgcatca ccagcttatg cgttggtctg ccacctttgg tcacgcgata
    50041 ttgattgtgg atctcagccg gaccgtccag cgataaccca acaagaaaat gattttctgc
    50101 cagaaatgca caccatttat catcgagcag cacgccgtta gtctggaagc tgttacttat
    50161 cttcctgcca gcaccgtatt tcgcctgtaa ctttacggca cggcggtaaa aatccagccc
    50221 gagtaacgtt ggttcgccgc cctgccaggt aaaagccact tcgttttgcg gttcgctggc
    50281 agcgatatag tgacggacat acgcttccag cgtgtcatcg tccatatgcg tgactggctt
    50341 ttcgcggtaa agggattgtt tttcgagata aaaacagtag tcacagttca gattacaatc
    50401 ggaaccactc ggtttcgcca tcacatgaaa agcacgcgtt ggaacctgtt gcagcatggt
    50461 cactccttgc ctgaagtcat ccttcaatac gttaagggcg aaatcatacc gtggggcaat
    50521 gagttattac gggcgtttat tgcgaaggga gggtgacgaa atgcacagaa aacaaaaaac
    50581 cccgccgaag cggggttcaa aattggtcgg cgagagagga ttcgaacctc cgacccactg
    50641 gtcccaaacc agttgcgcta ccaagctgcg ctactcgccg aaatactgct ttttgaattt
    50701 ttagttcaat tctttaaagt cgtggtgcga ggggggggac ttgaaccccc acgtccgtaa
    50761 ggacactaac acctgaagct agcgcgtcta ccaattccgc caccttcgca ttgtcacaac
    50821 ttctaataat ggggtggcta atgggattcg aacccacgac aactggaatc acaatccagg
    50881 gctctaccaa ctgagctata gccaccacta caaatcttgt tacgcggtat tactaccacc
    50941 gcagctcaag cgccgggact aaatggcgcg cccgacagga ttcgaacctg agacctctgc
    51001 ctccggaggg cagcgctcta tccagctgag ctacgggcgc ttagcgccgt tgcgggggtg
    51061 gataatacgg acttcccacc cctctgtcta gtcccttttt aaataaattg cgcgtttggt
    51121 tacggtttgt gcgttttgct gcttattcct ccagtttatg cgctttcccg tggcgattaa
    51181 ggccaaaaac tttataaatc gccgtcaccg ccagcataaa gatgatgcca acaaacagcg
    51241 acatacgcgt atcttcatta aagtacatgc cgatcaaaac gcaaatcagg aatgccattg
    51301 ttacgtagtt ggcccaaggg aacaggattg agcggaacgg atggctggca atcgccgctt
    51361 tatgtgcacg ccgaaaacgc agctggctta tcaatatcac aaaccatggc accatccccg
    51421 gaagcacgct ggcactgtag acgtagacaa acacacgctg cggattggga atgatgtagt
    51481 tcaggcatga gccaattagc agaatagcaa tagatactgc cacacccgca actggtacgc
    51541 cgtgacggga aactttcgcc atcgccgccg gtaactgacg gtttttcgcc agtgcgtaga
    51601 gcatacgtcc gcaactgtac atgccgctgt tacagccaga gagcgcagcc gtcagcacca
    51661 caaagttgat aatgcccgcc gctgcggtaa taccgatttt ggcaaaagtc agtacgaacg
    51721 ggctgccgtt gctgccaatt tcattccacg ggaagatggt gacgataacg aaaatcgcgc
    51781 ctacgtagaa aatcaggatc cgccacagca ctttgcctac ggcactgcgc agcgtcacct
    51841 gcggattctt cgcttcaccg gcagtaatgc caatcagctc cacgccctgg taggacgcca
    51901 ccacaataca cagcgcggtc aggaaccctt tccagccacc cgcaaagaaa ccgccatgct
    51961 ctgtgagatt gctaaaacca atcgactgcc cgccattgcc aaagccaaag aaaatcacgc
    52021 ccaggccaat gacaatcatc acgataatcg tggtgacttt gatcatcgcg aaccagaact
    52081 cgatttcgcc gtacaaccgc accgccgcca gattcgccaa cgccaccagc gccactgcga
    52141 tcaatgcggg tatccactgc gccatctccg ggaaccagaa ctggacataa acgccaatgg
    52201 cggtgatttc tgagatcccc accgccatcc acataaacca ataagaccag gcggtgagat
    52261 aaccaaagaa cgggctcata taacgatgcg cataaacggc gaacgaaccg gtaaccggtt
    52321 cgaggaacaa catttcgccc attgaacgca tgatgaaaaa gacgaatagc ccggcgatga
    52381 tataggccaa caatacggat ggcccggccc atttcaaggt actggcggcc cccataaaca
    52441 ggccgacgcc aatggtgccc cccagggcga tgagttcgat atgtcgagct tccagcccac
    52501 gctgtagctc tggtttgtta tctgccataa atcctcgtgt tgtgtttgca tgctttccgg
    52561 tgttaccggt tatcgttatg ggtacatcga gtgttgcgaa tgttttcgta attcaggaga
    52621 aatggcaaat aaagcattaa aaatttgaat gctttgtgta ataaaaaagc agacaggcga
    52681 cgaagtgacc actccgtcgc tttacaaaga gaggaaaatc agaggttgcc ggtgtaatgc
    52741 cagcgtaaat aacgcagcaa acgaagctga cgcttaatgc ggctcggctg cgaaagcagg
    52801 cggtagagcc actccagtcc cagcgtttgc cagattttcg gtgcgcgttt tacgtgaccg
    52861 gtgaaaacat cgtaagtccc gccaacgccc atatacagcg catctggatg caccagacgg
    52921 cagtcgcgca tgaagatctc ctgctttggc gatcccatcg caacggtgac gatttgcgct
    52981 ccgctggcat gaatgcgttc aaacagcgcc tgacgctgct cgggtttaaa ataaccatcc
    53041 tgactgccaa cgatattcac attccattgg ttgcgcagtt tagcttcagt ttgcgccagc
    53101 acttcaggtt taccgcccac aagaaatacc ggcgtccctt ctttgcctgc gcgcgccatc
    53161 agctcttccc agagatcggc accggcaacg cgggaaacct gcgcctgcgg gtacttttta
    53221 cgtactgaac gtacaacgct gatgccatcc gcatatttaa attcggcagc gttaattaac
    53281 tccctgacct cggcgttatc ttcaatggtc agcatttttt cggcattaat ggcaaccagc
    53341 gttccctgct taagctgccc gtcagcaaac agataatcga gggcgtgctg catatcacgc
    53401 caaccaatca actgtaagcc acgcagcgta taggtcggtg ccgtggtgtt gttattcatt
    53461 gttatccttc aacctgcgtc cggagcgatg attttgtacg tttatgaatg agtccggcgc
    53521 tttcaaaaag ccagtacaac agttttgcga tcatcagaca tgcgccgaag accacgataa
    53581 aaaagactac gcgtgagaca aacgaatcca gcccttcacg tgccagcacg atcatattga
    53641 agatcgcccc aaagcagaaa ctgtgcaata tcgcagcctt atagcgatta gtctcgcggt
    53701 tgcccagctc atacagccag tcgaaccatt tgatgatcag tccaaccaca atcgccccga
    53761 gtgggatgaa caacgcgccg cccatcacca ccagtgagcc tataagcgta ggcgagatcg
    53821 ccagtccgga gtggttattc agcacttccc aggtaaagta gttggctgag ttcagcacca
    53881 tactcgggcg acccggccac agccaggaag ggataaagac atagaaatcg cggacaattg
    53941 gagccaggcc ctggaagtcg atgttgtcgt agttctgcaa cagcaacgcc agattctccc
    54001 atggcgagaa ggtgtcgcga gtgagataga gaaacgtata gaacgcttca tcgccgctca
    54061 cattcattcc atagcgtttt agcgccagcc agaacatgcc cacaatcccc agcacgcccg
    54121 ccgccgccag catccacaac gaaatccagc cgcgaataat gccaataaac aggaagatag
    54181 cgaatgcgat gatgatattg gcgcgagtgc cgccgacaat catataagtc agcaagccaa
    54241 aggcgaccgt gctgacgagg aaaaacagcc acgctttgct atcctggcgc agaaagtaga
    54301 ccaccagcat cgccgggatg aaaaagtaaa agaagcgttt taacgccacg ccggagactt
    54361 cactggagaa gatctgactg taggagttaa gccggaacag caaaaagccg ttgtgcatga
    54421 agaagatgcc gacgcttacc agcgcgatgc ccatcaggat cacccacgta agattggtct
    54481 ccacgcggtt catggtaaac agcggacggc gcggtacatc agcaacgcgt ttgcgtaggc
    54541 gggttttgta ggtgacatag taaaccgcgt agaagcagcc cgcagaaagc aacgcctgca
    54601 acaagatttc tggcggcgcg acaccaacat caaagcgaaa taccagcacg ctggtcagcg
    54661 ggaagccgaa gaaaaaggtg agcaaaaaca gcaatgaaaa gaagacattg aagttaaagc
    54721 gcacacggcg aaactcaaac caggtcagcg tggcaataaa cagcgtgcag agcagccaga
    54781 caacaaacag gccactgaat tgcagcagac tcatgcgacc tccccggcgg caatcgccaa
    54841 cgcccgctgc cagccttgta gatagttagg gctaaagaag gcaatggtgt ttttatccac
    54901 cgacgccaac tggcgctgcg cttcacgcac aatatcctcg ttgagatcgt cggtagtaaa
    54961 cagcaccggc aaatgctgtt ccgtcatatc ctgccagaac ggattttccc ggttaagcac
    55021 acaaggaatg cccgcctgaa tcagtaagca aagcgtacca atgccctgct ggcgggcaaa
    55081 aataaagtaa ccaagatcgc actgacgaag tagcgccaga taggcgtcaa attccagttt
    55141 ttcgctcaaa atttgcagat tttcttcgct gaataactcc agccccgcct gacgaacttc
    55201 ctcaatgtac gcttcgttat taggcggata tcccatcggc accaccactt ttaccgtatc
    55261 gccaaattgc tgatgaacgg cgcacaaggc agcaatatgg tcattgctgc ggtcaccgga
    55321 gttccccacc agaatggtca ttttcccttc acgttgccga tcgttcgcca tcgtattgag
    55381 agaagggtcc atccgcgtcg ggaagtacag cagttcgccc cgcacctttg gatgcgtttt
    55441 ggcaaaaaag ctcaaatcgc cgcgggtggc aaatacacag ccgactcgct tttgcgccag
    55501 gcgacgtagt gggtaaaaaa gcttatatct caagccactg gaaagctcat acaggtctgc
    55561 cccccagata tgccagaaaa actggctggg cttaatgcca ccactcagca gagccagcca
    55621 cagtgtggga ttgaactgac cgtggaagaa aaaacgctgc tgacggttag cttttgcttt
    55681 cgcgatgacc gcttccgcca gcgatttttt cccagggaaa aattgcacag aaagcgccgg
    55741 acagctatca cttaagccgt cgtccttgcc aacaaccata aactcgcgcg cgtgctcgct
    55801 cgtcgcggcc agcgcgtcat tgaaaaaccg caaaacggtt cggttatggt gagggatatc
    55861 cgatcccagt acgtgaatca gtacagtcat gcccgcctac gccagagtaa aaacacgcca
    55921 caacaaagag aaaaatagac gatataagtt gccatatatg cctgcgccgc gcccagtgca
    55981 ccatgcgcag ggattagcca gtgggcaaat accatcaata aagtgaactg gctgacttcc
    56041 gccagaatat aaaaccgcag tgacgctttg gcgatcacca gataaccaaa gacataagcg
    56101 cccactttta acacatcacc cactaactgc caggcaaaga gatcgcgcat agcggtaaat
    56161 ttattcgaca acagcaacca gatagcaaaa tcacgcagca accagacggt aaaactcgcc
    56221 gccgccactg ccggtaagac gaatttcagc gatttaacca cttcccgggt gatatcgcgc
    56281 ttttccgtta gccgcgacaa cgtgggcagc aaatatacgc tgaacgatgc cgtaataaat
    56341 tgcaggtagg catcggaaat actgctcacc ccttgccaga tccccacctc atcccagcta
    56401 tactgcgccg ccagcagttt acgcatcatg atgtaagcaa caggcaaggt caccgacgta
    56461 atcaacgcca tgagcgtaaa tttgctcaac tgccctgcca gaccgttatc ccagctgggt
    56521 ttcagatagc ttaacgggat gacaccacgt ttgatcaaca tgatggcggc aggaatcacc
    56581 accagcgcgg gaatcagcgc cagacccagc aacgcccctt cataaccgcc caaacggtaa
    56641 ctgacgtagt acgcgagaac gccaatcaag ctgccgacaa tcagcgataa cgcattacct
    56701 gcggcatcgc gaaagccttt catcagcgcc agtaacaggt tgccccaggc gatccccatt
    56761 tgcaccagcg ccactaaacg caccagcccc tgatagtcgg tattaccaaa caatccctgg
    56821 ctgattggcg cagctgccag cacaaaaacc agcgccatca gcgtagagaa accaagtacc
    56881 atcgctgatg aagtgccgac cacgcggcgc agctgttgcg gattatcatg gtactgggca
    56941 acgtatttgg ttacaccgtt aaagatgcca gctccggcaa gcacgccgag cacggtaatc
    57001 aactggcgga aatttgccgc cagcccaagc cccgccggac caaatgacac agccagcaac
    57061 ttaccgacca gtaacccggc accaatcttg accagtgtac tggccgccgt ccacaaggac
    57121 gcttttgcca acgacatatc aggagaagta gttcaacaaa gtcgcaatta ccgtacgctg
    57181 attgacgggc gacaggttgt agaacagcgg caggcgcagc aggcgctcgc tctctttggt
    57241 ggtgtagcga tcttcaccgt ggaactcacc aaagcgttcc cccgcagggc aaccgtgcag
    57301 cggaatgtaa tggaacaccg ccatgatttc cgcttctttc agaaagttaa tcaacgcgct
    57361 ccggtcatca atatcccgca gtttaatgta gaacatatgc gcgttctgca cgcagccatc
    57421 gggaatcgac ggcagctcga tacgcccggc tttcgccaga ggcgctaacg catcgtagta
    57481 gttttgccac agcgccagac gttgctggtt gatacgatcc gctgcttcca gttgcgccca
    57541 caggtacgca gcttgcagat cggacatcaa atagctggag ccaatatcgc gccaggtata
    57601 tttatcgacc tgaccacgga agaactggct gcggttagtg cccttttcac ggatgatctc
    57661 ggctcgttcg attaacgctt tatcgttaat cagcgtcgcg ccgccttcac cacccgccgt
    57721 atagtttttg gtttcatgga agctaaagca gccaatatga ccaatggttc ccagtgcacg
    57781 ccctttgtaa gtggatatca cgccctgagc ggcatcttct accacaaaca gattatgctt
    57841 tttcgccaac gccataatgg tgtccatttc gcaggccaca cccgcgtaat ggaccggcac
    57901 gataacgcgc gttttgtcgg tgatcgccgc ttcaatcagc gtttcgtcga tgttcatggt
    57961 gtccgggcga acatccacaa aaacgatttt tgcgccacgc agcacaaagg cattggcggt
    58021 ggagacaaag gtgtagctcg gcatgatcac ttcatcgcca ggctggatat cgagcagcag
    58081 cgccgccatc tccagcgaag cggtgcagga cggcgtcagt aacactttgg cgctgccaaa
    58141 acgttgctcc agccactgct ggcagcgacg ggtaaaaccg ccatcgccac acagtttgcc
    58201 gctacccatt gccgactgca tatagtcgag ttcggttccc accaccggcg gtgcgttaaa
    58261 tggaatcatg tgatcacctg tataaccagt acgcggtgct ttctacattc gcaccacttt
    58321 gtatgtatcg tttaagcgcg gcggtgttgc ccatttgggt cgccacccgc aaagttgttt
    58381 taccgcgagc atacgcccag tttagcgccg tttgcatcag ctcagcacct acaccgcgtc
    58441 cagccagcag gccaattcgc gcatctgtcg cattgagttc ccgtaaagag acataaccgc
    58501 gaataccgcc ggacgccgca cgtaaaatca gacattgatg atcaaaggtg ccgcgcacgg
    58561 cattttcaat ccactgtgca taaaagcgtc tgctggcgtc aggcgcatac cacggcgcac
    58621 gaaaacggct ttgcgcaaat gcggcgctgg ctaactgacg taatgcggga atatcggtct
    58681 cttgtgccac tacagcacca ctatcactga cattgttcac gggtagtgcc aaatcaactt
    58741 caccttctac cagggagaat cccagctgtt gcagggcatc cagttcaccc gtatttgatg
    58801 ccgcaatttt ggcctgcacc cgtgaccacg gcgctaacac gtctggcgtc aggagcggtg
    58861 cttcagacgt aatgcgcacg atggcgctgt taacaccaaa gaaggcgttt tcccaggtta
    58921 gtggctcaat actggcgcgg acgggcacga agtaactcca gcagatattg gccgtagcca
    58981 gttttcgcta atgaactggc agcacgcttc acaccctcgt catcgagcca gccgttacgc
    59041 caggcgatct cttccaggca ggcaatctta aagccctggc gtttttctac tgtctgcaca
    59101 aaggtactgg cttcaatcag gctgtcgtga gtgccagtgt ccagccaggc aaatccgcgc
    59161 ccgagcagtt caacggtcag gttgcccgcc tcgaggtaca tctggttgat ggaggtaatc
    59221 tccagttcac cacgctccga cggcttcacc tgctttgcgt actccacgac tttactgtcg
    59281 tagaaataaa gcccggtcac cgcccagttt gacttcggct gttttggctt ttcttccagc
    59341 gagatagcgc ggaaattgtc gtcaaactcc accacgccaa agcgttccgg gtccatcacc
    59401 tgatagccaa ataccgtcgc cccttccgtg cgcgccgcaa catgacgcag cttcggactg
    59461 aagccctgac cgaagaagat gttatcgccc agcaccagac aagaaggttc accgttgagg
    59521 aaggtttcac cgatgataaa ggcctgcgcc agaccgtccg ggctgggctg ttcggcgtat
    59581 tccagctgga taccgaactc actaccatcg cccagcaggc gctggaaata acctttatct
    59641 tccggcgtag tgatgatgag aatttcgcgg ataccggcca acatcagcac cgacagcgga
    59701 tagtaaatca ttggcttatc gtaaatcggc aacagttgct tcgatacgcc gcgcgtaatc
    59761 ggatgcaatc gggtgccgga accgcccgcc aggataatac ctttcatgtt tgcctccgct
    59821 gaaaattagc ctttcagacc taaacgctcg ccctgatagc tgccgtcctg cacctgcttc
    59881 caccagcttt cattagccag ataccactgc accgttttac gcattccact ttcaaaggtt
    59941 tcctgtggca gccaaccaag ttcacgggca attttcgaag catcaatggc ataacgcaga
    60001 tcatgccccg gacggtcagc gacaaaggtg atcaagtcac gatagtgcgc cacgccgtgc
    60061 ggcttgttcg gagccagttc ttccagcagc tcgcaaatgg tttccacaac atcgagattc
    60121 ttacgctcgt tgtgaccacc aatattatag gtttcaccga ctttcccggt ggtcgccacg
    60181 caatacagcg cgcgggcgtg atcttccaca tacagccagt cacggatttg ctgcccgttg
    60241 ccatataccg gcagcggttt acccgccagc gcgttgagga tcatcagcgg gatcagtttt
    60301 tccggaaagt ggtaaggacc gtagttattc gagcagttgg tgatcagcgt cggcagaccg
    60361 taggtccgca gccaggcgcg caccagatgg tcgctgctgg cttttgacgc ggaataaggg
    60421 ctgctcggcg catacggcgt ggtttcggtg aagaaatcat ccgtcgagtg caggtcacca
    60481 tatacttcgt cggtggagat atgatgaaaa cggaacgctg attttttatc ttccgtcagc
    60541 gtattccagt aagcccgcgc cgcttcaagc aatgtataag tcccgacaat gttggtttca
    60601 ataaacgctg ccgggccgtc aatagaacgg tcaacatggc tttcggctgc cagatgcatg
    60661 acacagtctg gctgatgctc agtgaatacg cgtgccagtt ctgcccgatc gcagatatca
    60721 actttctcaa aggcaaagcg ctcgctttgc gcgaccggtg ccagcgacat caggtttccg
    60781 gcgtaggtca gcttatcgac cactaccacc gcgtcgctcg tttcgttgat gatataacgc
    60841 accagcgccg agccaataaa cccggcacca cctgttatca gaatttttct catcagcgcc
    60901 agactccttt ggcatcgacg acatactgct gatggacatt gtcgccattg ataactttga
    60961 actgactatg atcgaccagc atcaccagca cgtctgccgt tgccagcgcc tcgtcaagct
    61021 gcgccagagt acaaagcccg gtcagttttt tcggcaactg gtggatgtta ggctcaacaa
    61081 ccagagtttc gccgctatgc cactgggcga tcagttcagc gatttccatc gccgggcttt
    61141 cgcgcaggtc atcaatattc ggtttaaacg ccagaccaaa gcaggcgatt ttcagttcac
    61201 tggcgcgttt atcggtagca gccaggcaat cagccaccgc cgctttcacc tgatcgataa
    61261 cccagaacgg tttgtgatcg ttcacttcgc gcgcggtacg gataagccgc gcctgctggg
    61321 ggttctgtgc cacgataaac cacggatcaa cagcaatgca gtgaccgccc acgccagggc
    61381 caggctgaag aatattaacg cgagggtgac gattcgccag gcgaatcagt tcccagacgt
    61441 taatcccctg atcggcacaa atcagcgaca attcattagc aaaagcgata ttcacatcgc
    61501 ggaagctgtt ttcggtgagc ttacacattt ccgccgtccg cgagttagtg acgacacact
    61561 caccttcgag gaaaattttg tacagttcgc tggcgcgggc cgaacaaacc ggcgtcatac
    61621 caccaatcac gcgatcgttt ttaatcagct cgaccataac ctggcccggt aacacgcgtt
    61681 ccgggcagta agcaatgttg acgtccgcct gctcgcccac ctgctgtggg aaagtgagat
    61741 ccggacgcat ctctgctaac cattctgcca tcttctcggt tgaccccacc ggcgaggtgg
    61801 attcaaggat caccagcgcg ccttttttca gcactggcgc aatggagcga gcagccgatt
    61861 caacgtaggt catatctggc tcatgatcgc ctttaaacgg cgtaggtaca gcaatcagcc
    61921 aggcatccgc ttcaactggc gtcgtgctcg ctcgtaaaaa accgccttct acggcagttt
    61981 ttactacgct cgccaaatca ggttcgacga tatggatttc gccacgattg atggtatcaa
    62041 ccgcgtgttg gttgatatcg acaccaatca cctgtttttg ccgtgaggca aacgcagctg
    62101 ctgtaggcag cccgatgtaa cccagtccga taacagaaat ggtcgcaaaa ctcatagtga
    62161 tatccgatta ttttttaacg cttccagaat gcgagagcat gcctgaccat caccatacgg
    62221 gttatgggcg cggctcatag cttgatattc gttttcgtct tttaaaagac gcgtcacttc
    62281 ctcgacaatt cgctgcttat ccgtgcctac cagacgcacc gtacccgccg tcaccgcttc
    62341 cggacgctca gtggtatcgc gcatcaccag caccggtttc cccagcgaag gcgcttcttc
    62401 ctgaatgccg cctgagtcgg tcaaaatcag ccaggcgtgg ttcatcagcc agacaaacgg
    62461 taaatactcc tggggatcga tcagaatgac atttttcaca tgccccagaa tgcgattgac
    62521 cggctctctg acgttcgggt tgagatgcac cggatagaca atctggatgt cctggtgcgt
    62581 ggtggcgatg tctgccagcg cgtggcagat ttcttcaaag ccacgaccga aactttcacg
    62641 cctgtgaccg gtcaccagaa tcatcttttt atcggggtcg ataaacgggt aatttgccgc
    62701 cagttctgaa cgcagcgtgt cgctgctcat cacctgatca cgcacccata acagtgcatc
    62761 aatgactgta ttaccagtaa tgaagattcg gctatccgca acgttttcac gcagcaagtt
    62821 ttgccgggaa gtttcggttg gagagaagtg atacatcgcc agatgcccgg tcaatgtacg
    62881 gttagcctct tccggccacg gcgaatagag atcgcccgtg cgtaaaccag cctcaacgtg
    62941 accaacagga atacgctgat aaaacgccgc caggctggtt gccagcgtcg ttgttgtgtc
    63001 gccgtgaacc agcacgacgt ctggtttgaa ctcggcaaga ataggtttta gcccttccag
    63061 aatccgacag gttatctctg tcaggccctg tcctggctgc attatgttga gatcgtagtc
    63121 aggtacaatg gaaaagagtt tcagcacctg atcgagcatc tcccgatgct gcgcagtgac
    63181 gcaaacttta gcctcaaaaa aaggatcttt tgccaacgca tgcaccaacg gagccatctt
    63241 gatggcttcc gggcgcgtac caaatacagt cagtactttc acatcgattc tcttcgaata
    63301 agcggcgagc gcctttgcgc tcgcagcagc agtgttgcta tttcgagcaa cggcgggtta
    63361 atgcgacacc agccccgatc agccccccga caatgcccca cataatcatc aggaaggcac
    63421 gacgtgggct atcgcgtttt accggttctt ccggcgtacg caaatagcga taggtctgaa
    63481 aacgcggatc cagggttgga ccaacattca gggtgtttaa catggcccga ttctgatcat
    63541 agtcgagatc aaaggccgga ccgacggcct gtaaattttc cagtcgagcc tggagcattg
    63601 gacgcccaag caggaacatt tctgaatcag gtaattcctc ggcaggcaca tctgtcgcac
    63661 tgcgcgaaat attatgctgc tcagcaattt tcagcgcctg ctcaatgctg ttcatccggc
    63721 ggtcgtagat ggctttcgcc acctcttcct gacgcttcac ctgagctttc atctggatgg
    63781 tgcgtgccgc ccatgcgcct ttcagctcat cattcagatg gctggctgca cgctggctgg
    63841 caaaagcaac atactgacgt aacaggttat tagcgtcagg cgcggtttcg gcaataagct
    63901 tcacgctgtc attgaccgcg cgggtaaagt ctccggggat aaactggatg ttgttaatca
    63961 tttcatccag caacgccgca tcggctttgc tgttgcccac catccgctgt ttgtaatagt
    64021 cggtttgcag ccagaactct ctgcgggtat cccacgaggc cagttgcata acaaactctt
    64081 tataggcttc gtccatgacc gatggttgat cggcagaagc catgtttgaa cggacatcca
    64141 ggttacgcaa aaattgctgc tgcgagtaat atccccccag catattcacc gttggacgat
    64201 cggtaatcgc cgtcgagctc cactcctgac gagcaaaaaa agtatacgcc agcgcgatta
    64261 acgcaaacgc cagccccatg ccaataatcc atagcttccc agcccacaag gtacgaaaca
    64321 acccacgaat atccagttca ttttcagcgt cttcggccgg tttcccaggc attggttgtg
    64381 tcatcacatc ctcatttatt tggttaaatt ggggctgcca ccacgatttc tacgcagtct
    64441 gcgttttacg cgcttaataa agcgagcaac tttccaggca cgcttaatgc aatatccata
    64501 gaggaagaat gctagcaaaa agagcaccag catgacccac tccgggacaa aatgagaata
    64561 ttctgccagc acgccaatgg aagcgagcag tgctgcggca agggtaatca gcacaaacgc
    64621 ctgacgggaa gtaaacccgg cacgcatgat caaatggtga atatgctgac ggtcaggaga
    64681 gaatgggctc atgcctttac gcaggcgacg gtacataatc gccaccatat ccattagcgg
    64741 aatggcgatt atccacaaag cggtaaccgg gctgatggga tgggttttgc cctgggtcgt
    64801 ttcgagcagg atccagataa cggtaaaacc aatcagcgta ctgcccgcat cacccataaa
    64861 gactttgtag cggcgaccca ggataccaag gttaagcatg atgtatggca ggatggcggc
    64921 gatcatcgca aagcaccaga ttgcgaggct ggtttgcccg tcgaaccaca aaatcatacc
    64981 gattgctgca aacgagacgc aggacaaccc gcccagcaag ccatcaatgc catcaaccat
    65041 gttgaacgca ttaatggccg cccagacggc aaatagcgtc aggaagtaac caaacggtcc
    65101 gagcaccatc tcccaggagc caaagatata acccaggcta ctgagataaa gtttgccgaa
    65161 caccatcata acaatgccaa cagcggcctg tatggtggca cggattttta cgctgatatc
    65221 aaaacggtca tccagcgcgc caatgaaaac aagcacaccg gcacaagcga gatagagaga
    65281 tgcatgcgga atatagtaat cgacaattcc gaacgtgaag caaatccctg cgtaaaccga
    65341 aatcccccca acgagaggta tcaatccctg gtgacgtttg cggaagtttg gtttatccac
    65401 taaaccgact ttttttgcca ccttacgggc aaaaaacaga aacagtgtcg tgaataaaaa
    65461 aatactgatg agatcagtac tcactgtcag taaattcaca atgcatgctc tcagagaaaa
    65521 ttattagcag aagtataacc acgaagacct ttattcagaa gggaaagtcc gttccgaacc
    65581 caccagtcct gtgcaaaaat taaccatttg atatgcaaat tgcataacat gccagcaaat
    65641 tccaggaatt ttcctaacat cgccagcgcg gcattaagat tacagatata aaagcaaaac
    65701 gccacgtaaa cacgtggcgt ttttggcata agacaaattt atgagcgttt catcatttcg
    65761 aagaaatcgt cattggtctt ggtcatggcc agtttattaa tgaggaattc cattgcatcg
    65821 atttcgccca tcgggtgaat gattttgcgc aggatccaca ttttctgcag ttcttcctga
    65881 gtcgtgagca gctcttcttt acgggtaccg gaacggttgt agtcgatagc cgggaagacg
    65941 cgtttttcag cgatcttacg agagaggtgc agttccatgt tgcctgtacc tttaaactct
    66001 tcgtaaataa cttcgtccat tttagaaccg gtatcgataa gcgccgtcgc gataatggtc
    66061 aggctgccgc cctcttccac gttacgcgcc gcaccgaaga agcgtttcgg acgatgaagg
    66121 gcattggcgt ccacaccacc ggtcaacact ttacctgacg ccggaacaac agtgttgtaa
    66181 gcgcgcgcca gacgagtgat ggagtcgagc agaatgataa cgtctttctt gtgctcaacc
    66241 aggcgtttgg ccttctcgat caccatttcc gcaacctgaa cgtggcgaga tgcgggttcg
    66301 tcaaaggtag aagcaacaac ttcacctttt accagacgct gcatctcggt tacttcttcc
    66361 ggacgttcgt cgatcagcag aaccatcagc acacaatccg ggtggttgta agcaatgctc
    66421 tgagcaatgt tctgcagcag catagtttta ccggctttcg gcggtgccac aatcagacca
    66481 cgctgaccac gaccgatagg tgatgccaga tccagtacgc gagcggttaa atcttccgta
    66541 gaaccgttac cacgttccat acgcagacga gagtttgcgt gcagcggggt taagttctca
    66601 aagaggattt tgttgcgggc gttttcaggt ttgtcgaagt taacttcgtt aactttcagc
    66661 agcgcaaaat agcgttcacc ttctttcggc gggcgaatct taccagagat ggtatcacca
    66721 gtgcggaggt tgaaacggcg gatttggcta ggggaaacgt agatgtcatc aggaccggcg
    66781 aggtaggagc tgtctgcgga acggaggaaa ccaaatccat cctgcaatat ctccagtacg
    66841 ccatcaccaa agatatcttc gccactcttt gcgtgctgct tcaggatggc aaaaataatg
    66901 tcctgcttac gcatacgagc caggttttcc agccccatat tttcgccgag agtgatcagc
    66961 tcagaaaccg gcgtattctt taattcggta agattcatag tggtgtgagt tcttaaactt
    67021 ggggtaaatc tcgaacttaa tgttgtgaat ggtatggcag gatcatccat gcctgtttac
    67081 ggtcatcgac tcatgtcttt tcgctgcctg gtcacaggag agtacgcaga actgaaacga
    67141 caagacggat tgagtgacga gccagaaatc tgttcacttc gcatttaaga taaaacggga
    67201 agcgttggaa attacaagat tcaaacttaa taaggtatgt ttaatacgaa gtcaacacta
    67261 agttagcatg actcacgccg ggcgtccagt ttttagcgac ggggcacccg aacatgaaat
    67321 tcccttacgc caggttagcg tcgaggaact ctttcaactg acctttagac agtgcaccca
    67381 ctttggttgc cgccacttca ccgtttttga acagcagcag agtcgggata ccacggatgc
    67441 catatttcgg cgcagtgcca gggttttgat cgatgttcag ttttgcaacg gtcagtttgc
    67501 cctgatattc gtcagcgatt tcatccagaa tcggggcgat cattttgcac ggaccgcacc
    67561 actctgccca gaaatcgacg aggatcgccc cgtccgcttt gagtacatcc gtgtcaaaac
    67621 tgtcgtcagt caggtgaata attttatcgc tcatatataa ctccacagga ataagcctgg
    67681 cgtgttggtt tcgttgttgg tgtaacatta accaactaaa ggttgacttt atttcaccgg
    67741 atacgctttc gtaaagcaat agtaagctga tattctacca cactatgagc aaaacacatt
    67801 taacagaaca gaagttttcc gacttcgccc tgcatccgaa ggttgtagaa gcccttgaaa
    67861 aaaaagggtt tcataactgt acgcccattc aggcactggc ccttccgctg acgctggcgg
    67921 gtcgtgacgt agccgggcag gcgcaaaccg gtaccgggaa aacgatggcg tttcttacgt
    67981 caacgtttca ttatcttctc tctcatcctg cgattgccga tcgcaaggtg aatcaaccac
    68041 gtgccttaat tatggcaccg acgcgtgaac ttgccgtgca gatccatgcc gacgcagaac
    68101 cgctggcgga agctaccggc ctgaagctgg gtctggctta cggtggtgat ggctacgaca
    68161 aacagctgaa agtgctggaa agcggcgttg acattctgat tggcactacg gggcgtttaa
    68221 ttgactacgc caagcagaac cacattaacc tcggtgccat tcaggtggtg gtactggacg
    68281 aagccgatcg catgtacgat ctgggcttta ttaaagatat ccgctggctg ttccgccgta
    68341 tgccgcctgc aaaccagcgc ctcaacatgc tgttctccgc cacgctttcg taccgggtac
    68401 gtgaactggc gttcgagcag atgaacaatg ccgaatatat tgaagtggaa ccggaacaga
    68461 aaacgggcca ccgtattaaa gaagagcttt tctacccttc taacgaagaa aaaatgcgtt
    68521 tgctgcaaac gctgatcgaa gaagagtggc cagacagagc gattattttc gccaatacca
    68581 aacaccgttg tgaagagatc tggggccacc tggcggcaga tggtcatcgt gtcggtttgt
    68641 tgacaggcga tgtcgcacag aaaaaacgtc tgcgtattct tgatgaattt acccgtggcg
    68701 atctggatat tctggttgcc accgacgttg ccgcgcgtgg tttgcatatt ccggcagtga
    68761 cgcacgtctt taactacgat ttacccgatg actgtgaaga ttacgttcac cgtattggtc
    68821 gtacaggtcg cgcaggcgca agcggtcact ctatcagcct ggcgtgtgaa gagtatgcat
    68881 tgaatttgcc tgctattgag acctatattg gtcactcaat tccggtaagc aaatacaatc
    68941 cggacgcatt gatgaccgat ctgccaaaac cgctgcgcct cacgcgccca cgtacaggca
    69001 atggtccgcg tcgtactggc gctccgcgta atcgtcgtcg ttcaggttaa ataaaatgtc
    69061 atactcaaaa tcattcaaac tgtatcaaga tggcaagaga atgaatcccg atgagcatac
    69121 tgtagtaagt gattcaggtg aaagaatgca gccaacacag agacagattg aaggatgaag
    69181 agtatgggtt ccacctcgtc gctgtatgca gccattgatc tcggttcgaa tagttttcat
    69241 atgctggttg tgcgcgaggt ggctggaagc atccagacgc tgacgcgaat taaacgcaaa
    69301 gtgcgtctgg ctgctggcct gaatagcgaa aatgccctct ctaatgaagc aatggagcgc
    69361 ggttggcaat gtctgcgcct gtttgctgaa cgtctgcaag atatccctcc ctcgcaaatt
    69421 cgcgttgtcg ctacggcgac attacgtctt gccgtcaatg cgggtgcttt tattgccaaa
    69481 gcgcaggaaa tcctcggttg tccggtacag gtgatcagcg gtgaagagga agcacgtctg
    69541 atttatcagg gcgttgctca cacaactggc ggtgccgatc agcgcctggt ggtggatata
    69601 ggcggtgcca gtactgaact ggtaaccggc acgggtgcac aaaccacctc gttgttcagc
    69661 ctgtcgatgg gctgcgtcac ctggctggaa cgctattttg ccgatcgtaa tctggggcag
    69721 gaaaattttg atgctgcaga aaaagcggca cgcgaagtgt tacgtccggt tgccgatgaa
    69781 ttacggtatc acggctggaa agtgtgcgtt ggtgcttccg gcaccgtgca ggcgttacag
    69841 gaaatcatga tggcacaggg gatggatgaa cgcattaccc tggaaaagtt gcagcaattg
    69901 aaacagcgag ccattcattg cggtcggctg gaggaactgg agattgacgg gctgacgctg
    69961 gaacgtgcgt tagtgttccc gagtggtctg gcgatcctga tcgccatttt taccgaactg
    70021 aatattcagt gtatgaccct ggcgggcggt gcgctgcgtg aaggcctggt ctacggcatg
    70081 ttgcatctgg ccgtcgagca ggatattcgc agccgtacgc tgcgtaatat tcagcgccgc
    70141 tttatgatcg atattgatca ggcgcagcgc gtagccaaag ttgcggctaa cttcttcgat
    70201 caggtggaaa aggaatggca tcttgaagca ataagccgcg atttgctcat cagcgcctgt
    70261 cagcttcatg aaatcggcct gagcgttgac tttaaacaag cgccgcaaca cgctgcttat
    70321 ctggtgcgta atctggatct tcccggtttt acccccgcac agaaaaaatt gctggcgacg
    70381 ctactgctca accagactaa tccggtcgat ctctcatcgc tgcatcagca aaatgccgta
    70441 ccgccgcgcg tcgcagaaca actctgccgt ttactccgcc tggccatcat ttttgccagc
    70501 cgtcgccgtg acgatctcgt gccagagatg acattacagg ctaaccatga actgttgacc
    70561 ttgacgcttc cgcaaggttg gctaacccaa catccgctgg gtaaagagat tattgctcag
    70621 gaaagccagt ggcagagcta tgtccactgg ccgctggaag tgcattaatg agtaagtgcc
    70681 ggatgcgatg ctgacgcatc ttttccggcc ttgattattt ccctcgtttt gccgccatca
    70741 tcgctttcag attcgccaga tggctttgcc ctttctgcat ccgttcttcg gcgctgacca
    70801 ctttgcgctc ctgttcccaa atcagatcat cctgcggcag ctccagcaaa aagcggctcg
    70861 gctccgggcg caccagctcg ccgtactgac ggcgttcttt acacagcgta aaggtcaatt
    70921 ccttctgggc gcgggtaatg ccgacatagg ccagccgccg ctcctcatcg atattatctt
    70981 catcgatgct gctctggtgc ggcaaaaacc cttcttccat accgaccatg tagacataag
    71041 gaaactccag cccttttgac gcgtgcagag tcatcagttg cacctgatcc agctcttctt
    71101 cactctcacc acgctccatc atgtcgcgca aagtaaagcg cgtcaccacc tgggtgagcg
    71161 tcatcggctc atccagttca ctgccttcca gcatctccgt catccagcta aacagttggt
    71221 tgacgttctt catgcgcatt tcggcggctt tcgggctggg cgatgtttcg tacagccagg
    71281 attcataatc catgccatgg atcagatcac gcaccgcagc aatcggctcc cgctccgcca
    71341 ggcgctggat ttctgccaac cagtgagtga agcgggtcaa tgcttcataa ccacgtccgc
    71401 taagcgtctg actcaggccc atatcaaagc tggcggtaaa catgctttta ttgcgcgtca
    71461 tcgcccactc acccagcttt ttcagcgtcg ccgggccaat ctctcgcttc ggcgtgttaa
    71521 cgatacgcag aaatgcgctg tcatcgtccg ggttagtcag cacgcgcaga taagccagca
    71581 agtccttgat ttcaggacga gagaaaaacg acgtaccacc agaaattttg tacgggatgc
    71641 ggttttgcat caggaatttt tcaaacaccc gcgactgatg gttaccgcga taaagaatgg
    71701 cgtaatcttt gtactgcgtt ttattgacga agtgatgggc gatcagctcg ccagtaacgc
    71761 gctcggcttc atgttcctcg ttattcgcgc ttaaaacttt tagctctgtg ccgtaaccca
    71821 gttcggagaa cagacgcttt tcaaagacgt gcggattatt ggcgatcagg atgttcgccg
    71881 ctttcagaat acgcccggaa gagcgatagt tctgctcaag cttaatcacc ttcagcgccg
    71941 gaaaatcctg actcagcagc accaggtttt gcggacgtgc accgcgccag gagtagatcg
    72001 actggtcatc gtcacccacc acggtaaagc gcgcgcggct gcccaccagc agtttcacca
    72061 gttcatactg gctggtgttg gtgtcctgat actcatccac cagcaggtag cgaattttgt
    72121 tctgccagcg ttcgcgtact tcttcattgc gttgcagcag caacgtcggc aataaaatca
    72181 ggtcatcgaa gtcgagaacg ttacaggctt tcaggtgtgc atcatacagc ccataacaat
    72241 gggcaaaaat acgatcccgc tcgccctttg cctctgccgc cgcctgcgcc ggagttttga
    72301 gatcgttctt ccagttagag atggtcgaaa tcagttgttg caggagaact ttgtcatctt
    72361 caatcagccc ctcggtcaac tctttaagca aagcaagctg atcggtatcg tcaaacaacg
    72421 agaagttcgc tttcatccca agcgccgcat actcgcgttt gatgatatcc agccccaacg
    72481 tatggaaagt ggagatcatc agcccacgcg cctctttgcg ccccagcgtc tgccctacac
    72541 gctctttcat ctcgcgcgcc gccttattgg taaaggttac cgccgcgata tgtcgcgcct
    72601 gataaccgca accgcggatc agatgggcga ttttattggt gataacacga gttttaccgg
    72661 aacccgcgcc cgccagcacc aggcaggggc cggtaacgaa ttcgacagct tgttgttggc
    72721 cggggtttag acgcataggt gtattgctca atcttcgaac ggggggagga ttgtagcaga
    72781 atcgcatttg aatgtgaccg cgtgtaagcc gctaaccggt tggctgcgga tgtttaaatc
    72841 agagagagaa gaccgaaatc ggagttcatc gtgaatccat tagacttaga aaatatcggg
    72901 tcgtaacgtt gtgttattac tttttcagag tttactaagg agaggtttac tcgtggcgat
    72961 taatttcagc cctaaggttg gtgaaatact ggaatgcaac tttggtaact atcctgttag
    73021 ccaaaatgga ccgttcagca cgacgtatta cgatggccgc attcctcctg aaatgataaa
    73081 aaacaggtta gttgtcgtgc tcaacggaaa aattaatggc aacgcatgta tcgtcgttcc
    73141 attatcaacc actcgcgatc atgacaagtt aaatcgtgga atgcatgttg aaatagccag
    73201 taatgttatc aatggcctgc aattctttga tcagcagatt cgttgggcca aagccgacct
    73261 ggtacaacaa gtcagtcgaa accgattaaa tagagcaagg acttacagag ggtatcttaa
    73321 tcaatgtttg ccacatgagt tggttgcaga catacagagg gcggtcatca agtcaatcaa
    73381 tgccatatcg ctcataaatt gacaataaaa taaccatccc cgataatgac tttgtagccc
    73441 atagaggccc taagttttct ccccttgagg gacctgcgaa gcccggattt tccgggcttt
    73501 tgccatatat gtaccagccc ctccctgact gacaattacg ttagcatctg cggatcatgc
    73561 taaaatcgcc gcctgacaat tattcactca aggcacgatc atggcaaaaa cagcagcagc
    73621 actgcatatc cttgtaaaag aagagaaact ggctctggat cttctcgagc agattaagaa
    73681 cggtgccgat ttcggcaagc tggcgaagaa acactccatt tgcccatcag gcaaacgcgg
    73741 cggtgattta ggtgaattcc gccagggtca gatggttccg gcgttcgata aagttgtgtt
    73801 ctcttgtccg gtactggagc cgaccggccc gctgcacacc cagttcggtt accacatcat
    73861 taaggtgctg taccgcaact aatagcaagg ccttctccag gagaaggcct tgagtgtttt
    73921 ctccctctcc ctgtgggaga gggtcggggt gagggcatca gcgcgcactt aacccgcaac
    73981 agcaatacgt ttcatatctg tcatatagcc gcgcagtttc ttacctacct gctcaatcgc
    74041 atggctgcga atcgcttcgt tcacatcacg cagttgcgcg ttatctaccg cgctttccgg
    74101 aatagcttta cccaagtcgc ccggttgcag ctctgccata aacggtttca gcaacggcac
    74161 acaagcgtaa gagaacagat agttaccgta ctcagcggta tcagagataa ccacgttcat
    74221 ttcgtacaga cgcttacggg cgatggtgtt ggcaatcagc ggcagctcgt gcagtgattc
    74281 ataatatgca gactcttcaa tgatgccgga atcgaccatg gtttcgaacg ccagttcaac
    74341 gcccgctttc accatcgcga tcatcagtac gcctttatcg aagtactcct gctcgccgat
    74401 tttgccttca tactgcggcg cggtttcaaa cgcggttttg ccggtctctt cacgccaggt
    74461 cagcagtttc ttatcatcgt tggcccagtc cgccatcata ccggaagaga attcgccgga
    74521 gatgatgtcg tccatatgtt tctggaacag cggtgccatg atctctttca gctgttcaga
    74581 aagcgcgtaa gcacgcagtt tcgccgggtt agagagacgg tccatcatca gggtgatgcc
    74641 gccctgcttc agcgcttcgg tgatggtttc ccaaccgaac tgaattagtt tttctgcgta
    74701 tgccggatcg gtgccctctt ccaccagctt gtcgaagcac agcagcgagc cagcctgcaa
    74761 cataccgcac aggatggttt gctcacccat caggtcagat ttcacttccg caacgaagga
    74821 cgattccagc acgcccgcac ggtgaccacc ggttgctgcc gcccatgctt tagcgatcgc
    74881 cataccttcg cctttcggat cgttttccgg gtgaacggca atcagcgtcg gtacgccgaa
    74941 tccacgtttg tactcttcac gcacttcggt gccagggcat ttcggcgcaa ccatgactac
    75001 ggtgatatct ttacggatct gctcgcccac ttcgacgatg ttgaaaccgt gcgagtaacc
    75061 cagcgccgcg ccgtctttca tcagtggctg tacggtgcgc actacatcag agtgctgctt
    75121 gtccggcgtc aggttaacca ccagatccgc ctgcgggatc agttcttcgt aagtaccaac
    75181 tttaaaaccg ttttcggtcg ctttacgcca ggatgcgcgc ttctcggcaa tcgcttcttt
    75241 acgcagagcg taggagatat cgagaccaga atcacgcatg ttcaggccct ggttgagacc
    75301 ctgtgcgcca cagccgacga tgaccacttt tttaccctga aggtagctcg cgccatcggc
    75361 gaattcatcg cgccccataa agcgacattt gcccagctgt gccagctgct ggcgcaggtt
    75421 cagtgtattg aagtagttag ccatggtgat tcctcgtgat gttgtgcttc ttattgttcg
    75481 gttcgcgttt gcgagattga attcactata tgacaggaaa tttattgcgg aaattgatat
    75541 attcacaacg tcacattgca atttctgcaa cgtcaacatt gagggctgcg ccattggatt
    75601 tacgcgatct gaaaaccttc ctgcatctgg cggaaagccg ccattttggc cgcagcgcgc
    75661 gggcgatgca cgttagcccg tccacgctct cacggcagat tcagcgcctg gaagaagatc
    75721 tcggtcagcc gctgtttgtg cgcgataacc gcacggtgac gctgactgaa gcgggcgaag
    75781 agttgcgcgt tttcgcccag caaacgctgt tgcagtatca gcagttgcgc cacaccatcg
    75841 atcagcaagg gccatcgctc tctggcgaat tacatatctt ctgctcggtg accgctgcct
    75901 acagccatct gccgccgatt ctggatcgct tccgcgcgga acacccgtcg gtggagatta
    75961 aactcactac tggtgatgcg gcagatgcga tggaaaaggt agtcactggc gaagcggatc
    76021 tggcgattgc gggtaaaccg gaaaccttgc ccggcgcagt ggcgttttcg atgctggaga
    76081 atctggcagt agtgctgatt gcccccgcgc tgccctgccc ggtgcgtaat caggtgtcgg
    76141 tagagaagcc ggactggtca acggtgccgt ttatcatggc cgatcagggg ccggtacgcc
    76201 gccgcattga actgtggttt cggcgtaaca aaatcagtaa cccgatgatt tacgccacgg
    76261 ttggcgggca tgaagcgatg gtatcgatgg tggcgctcgg ctgtggcgtg gcgctgttgc
    76321 cggaagtggt gctggaaaac agcccggaac cggtgcgtaa ccgcgtgatg atactagagc
    76381 gaagcgatga gaaaacgccg tttgagctgg gcgtgtgtgc gcagaaaaag cggctgcatg
    76441 agccgctgat tgaagcgttc tggaagattt taccgaacca ctaacccgcc aaaaagaacc
    76501 tgaacgccgg gttattggtt tcgtcgtggc aatcgtagcc cagttcattc agccgggttt
    76561 cgaaatccgg ttcatggtcg ccaagctcga acgccgccag tacgcgcccg tagtcggtac
    76621 cgtgactgcg ataatggaac agagaaatat tccagtgcgt acccagcgtg ttgaggaagc
    76681 gcagcagcgc acccggcgat tccggaaatt cgaagctgta gagacgttcc tgcaacggat
    76741 gcgatggacg cccgccaacc atatagcgca catgtagctt cgccatttca tcgtcggaga
    76801 gatcaaccac gctgtagccg ccgtcgttga gcatctgcaa aatttctttg cgctcttcga
    76861 ggccgcggct taagcgcacg ccgacaaaga tgcaggcgtt tttggcatcg gcaaagcggt
    76921 agttgaattc ggtgaccgaa cgtccgccaa gcaactggca gaatttgagg aagctacctt
    76981 tctcttccgg aatggttacc gccagcaacg cttcacgctg ttcacccagc tcacaacgtt
    77041 ccgagacgta gcgcaggccg tgaaagttca cgttagcacc ggaaagaata tgcgccagcc
    77101 gttcgccgcg aatgttgtgc tgggcgatat attttttcat tcccgccagc gccagcgcgc
    77161 cagagggttc cgccaccgca cgcacatctt cgaataaatc cttcatcgcc gcacagatcg
    77221 catcgctatc gacggtgatg atgtcgtcga gatactcctg gcataaacgg aaggtttcgt
    77281 caccgatgcg ttttaccgcg acaccttcgg cgaacagtcc cacgcgtggc agatcaaccg
    77341 gatgacccgc atccagcgct gctttcaggc aggcggagtc ttccgcttct acggcgatca
    77401 ctttgatttg cggcatcagt tgtttgatca gcaccgctac gcccgccgcc agaccgccgc
    77461 cgccgactgg cacaaatacg cggtcgagat gggcgtcctg ctggagcagt tccagtgcca
    77521 gcgtaccttg cccggctatc accatcggat ggtcgaacgg cggcacccag gtgaagccct
    77581 gctgctgcga cagttcgatt gctttggctt tcgcttcgtc aaagttcgcg ccatgaagca
    77641 gcacttcgcc gccgaatcca cgtaccgcat cgactttgat atcggcggtg gcggttggca
    77701 tgacgatcag cgccttcacg cctaaccgtg cggaagaaaa cgccacaccc tgcgcgtggt
    77761 tacccgcaga agcggtgatc acgccgtgcg ctttttgttc ttccgtcagg cccgccatca
    77821 tggcgtacgc gccgcgcagc ttaaagctat gaactggctg gcgatcttcg cgcttcacca
    77881 gaatgacgtt atcaagacgc gacgacagtt tttccatttt ctgtagcggc gtgacctgcg
    77941 ccgcttcgta gaccggcgcg cgcagcaccg ctcttaaata ttcggcacct tcaggggcac
    78001 cggacagggg ttgtgagtct gccatcatta accccccagt ttcgatttat cgcgcaccgc
    78061 gcctttgtcg gcgctggttg ccaggctggc gtaggcacgc agcgcaaagg agacctgacg
    78121 ttcacgattt ttcggcgtcc aggctttgtc accccgagct tcctgcgctt cacgacgcgc
    78181 cgccagttcg gcatcgctta cctgtaactg aatgccacgg ttcgggatgt cgatagcgat
    78241 caggtcacca tcttcaatca gaccaatgct gccgccgctt gccgcttccg gtgagacgtg
    78301 gccgatggaa agaccagagg tgccgccaga gaaacgaccg tcggtgatca gcgcacaggc
    78361 tttaccgaga cccattgatt tcaggaagct ggttgggtag agcatttcct gcatgccagg
    78421 gccgcctttc gggccttcat agcggatcac caccacatcg cccgcgacaa ctttgccgcc
    78481 gagaatcgct tctaccgcgt cgtcctggct ttcgtacact ttcgccgggc cggtgaattt
    78541 gaggatgctg tcatcgacgc ccgcggtttt aacgatgcag ccgttttctg cgaaattacc
    78601 gtagagcacc gccaggccgc cgtctttgct gtaggcgtgt tccagcgagc ggatacagcc
    78661 attggcgcga tcgtcatcga gagaatccca acggcaatcc tgcgagaatg cctgtgtggt
    78721 acgaatgcct gcaggacctg cgcggaacat attttttacc gcgtcatcct gggtcagcat
    78781 aacgtcgtat tgttccagcg tttgcggcaa cgtcaggcca agtacgtttt tcacatcacg
    78841 gttcagtaac cccgcgcgat ccagttcgcc gagaataccg ataacaccac cagcacggtg
    78901 aacatcttcc atatggtatt tctgggtgct cggcgcaact ttacacagct gtggaacctt
    78961 gcgggaaagc ttatcgatat cactcatggt gaagtcgatt tccgcttcct gcgccgccgc
    79021 cagcaggtga agtactgtgt tagtcgatcc acccatcgcg atatccagcg tcatggcgtt
    79081 ttcaaacgcc gccttactgg cgatattacg cggcagtgca ctttcgtcgt tttgctcgta
    79141 ataacgtttg gtcaattcaa caatgcgttt accagcatta aggaacagct gcttacggtc
    79201 ggcgtgggtt gccagcagcg agccgttgcc cggctgcgac aggcccagcg cttcggtcag
    79261 gcagttcatt gagttagcgg taaacatccc ggagcaggaa ccgcaggtcg gacacgcgga
    79321 acgttcaacc tgatcgctct gggagtcaga tactttcggg tctgcgccct ggatcatcgc
    79381 atcaaccaga tcgagcttga tgatctgatc ggaaagtttg gttttcccgg cctccatcgg
    79441 gccgccggaa acaaagatca ccggaatatt caggcgcagg gaagccatca gcatccccgg
    79501 ggtgattttg tcgcagttag agatgcagac catggcgtcg gcgcagtggg cgttgaccat
    79561 atactcaacg gaatcagcga tcagttcgcg agatggcagt gaataaagca tccccccgtg
    79621 gcccatggca atcccatcat ccaccgcaat ggtgttgaac tctttggcaa cgccgccagc
    79681 cgcttcaatt tgttcggcga ccagtttacc gagatcgcgc agatggacgt gacccggtac
    79741 aaattgggtg aacgagttca caaccgcgat aatcggcttg ccaaaatcgg cgtcggtcat
    79801 tccggtggcg cgccacagcg cacgagcacc cgccatatta cgaccatgag tggtggtggc
    79861 ggaacggtac ttaggcatac tttatttact cccagtgtct gtctcgtaaa tgggacggtg
    79921 cgtgccgtcc cattttttgt atttattgat taacttgatc taaccagccc catttatctt
    79981 cggtttcgcc agtgaagagg ccgaagaagg cttgctgaat gcgtttggta accgggccac
    80041 aacggccttc gccaacctga ataccgtcta cgctgcgcac tggcgtgatt tctgccgcag
    80101 taccggacat aaacacttca tccgccaggt acagggattc gcgcgacaga acctgctcac
    80161 gcacttcaat tcccagctct ttcgccagtt tgatgatggc atcacgggta atacccggca
    80221 gcgcggagga ggtgaacggt ggggtgaaca gcacaccatc tttcacttca aacaggtttt
    80281 cgcctgcgcc ttcagagata taaccgttca catccagcgc gataccttcc tgataaccgt
    80341 ggcggcgcgc ttcgctaccc accagcaggg aagagagata gttaccaccg gcttttgccg
    80401 cggttgggat ggtgtttggt gctgcgcggt tccaggagga aaccatcgca tcgatcccct
    80461 gctccagcgc ttctgcgccc agatacgctc cccacgggaa agcggcgata atcacatcgg
    80521 ttgagtatcc cgctggcggg tttacaccca tgccaacatc accaacgaag atcagcggac
    80581 ggatataggc gctggtgaga ttgtttttgc ggatcacgtc acgacaagct tccatcagct
    80641 catcaatgct ctgcgaaacc gggaagcgat agattttggc ggagtcatgc agacgctgca
    80701 tatgctcacg atggcggaat acaaccggtc ctttgtgcga gtcgtagcaa cggatgcctt
    80761 caaaaaccga agtgccatag tgcagcgcgt gcgacatcac atgcaccttc gcgtcttccc
    80821 agcgaaccat ctccccattg aaccaaatgt aatcagcttt cttcgtggtc atttttatat
    80881 tccttttgcg ctcaggcgcg gatttgttgt gatgtggttg tgctctgaca gatggcaacg
    80941 tgtgcgacgt ccaccagttt atttaactga ctaaacagta agtcgaccga ccgtgggctg
    81001 gcaacggtca attcgatatt tatattttgt gcatcgctgg cggcggccat attcattgag
    81061 cagacgtgga aaccacgatg acgcaccacg cgtaaaacac gttctaaggt ttctggattg
    81121 aagcgagccg atacattgac ctgatgttgc atcatgataa tttctccaac atttctgaat
    81181 tactggcgcc aggcggcacc agcggccaga cgttctcaag ttcgtcgatt gagacatgaa
    81241 gcaggtatgg cccatcactg ttcagcatgg tgtcgagtgc cgcttcaacc tggtctttac
    81301 gggtgatgtg ttggccaggg atgccgaagg cgctggctaa catgaggaaa tcggggttat
    81361 cagtaagggt ggtttcgctg tatcgttcct gaaaaaacag ttgctgccat tgtcgaacca
    81421 tccctaaccg ttggttatcg agtaagacga ttttcaacgg taactgcttg cgttttacgg
    81481 tgcccagctc ttgcacattc atcatgaaag agccgtcacc ggagatacag acaacggtat
    81541 cgttcggtcg cgcgacttgt gcgccaaccg ccgccggtaa accaaaaccc atggtaccta
    81601 agccgctgga ggtgatgaaa ttttccgggc gagtgtgggc gatgtgctgc gcagcccaca
    81661 tctggtgctg ccccacatct gtggtcacga cgcaatccgc aggtttacga tccgacagtt
    81721 gttttaacaa caatggcgcg tagatagcgt caccgggatg gtcgtaacgc caggcatgtt
    81781 catcacgcag ctgcgcgcag tgtagctgcc agtcattgat atttaacggc tgctgtaatg
    81841 ctggtaacag agcatttaaa tcaccttgta atgccacatg tgcctgacgc agcttgttca
    81901 tttctgccgg gtcgatatcc atatggataa cactggcgtg tggtgcgaag gtgttcagtt
    81961 tgccggtcac ccggtcatca aaacgtgcac ccacggcgat cagcaagtcg cactcctgca
    82021 ccgcgaagtt cgccgctttg gtgccatgca ttcccagcat gcccagatag tacggataat
    82081 ctgcttcaac tgcgcccagc cctttcagcg tgcaggtggc aggcattttt gtggtagcga
    82141 gaaattctcg taaagcagga actgcctgcg ccatacccac gccaccacca acgtacagca
    82201 tcggtttttg cgcttttgcc agcatctggc gcgcttgctc gacttcggca tgtgggaaag
    82261 tcacttcgtt ttcaacggtg gtgaaccacg gttccaggtc accgctggct agctggatat
    82321 cttttgggat atcgaccaga accggaccag gacgacctga gctggcaacg tcgaatgctt
    82381 cagccatgat gcgcggcaat tcttccagcg actgcaccag aaagctgtgc ttggtacagg
    82441 ctaacgacaa tcccaggaca tccacttcct gaaatgcgtc agtgccgata aacggtgcgg
    82501 acacttgacc ggtgatggca acaacaggga tagaatctaa cagtgcgtcc gcaagcccgg
    82561 ttatcaggtt ggttgcgccc ggaccagacg tggcgataca tacgccagtt ttgccggtag
    82621 cacgggcata accgatagcc gccattgccg caccctgctc atgtcggcac agcaagtgct
    82681 ccacgccgcc gtcatacaat gcatcgtaaa ccggcataat tgcgccaccc ggataaccga
    82741 aaacggtgtt cacaccctgt gcccgcaacg catgtaccac ccactgtgcg ccattcatag
    82801 ttagttcccc gtcctgaatc ttgagaaaca gaattttgtg ctgttattca ttgtctgctc
    82861 ctcggttatg tttttaaggt caaaaaaaac ccccggacct ttcggtgcgg gggtcttagt
    82921 tcgttaaggc ttgatctcta agcctttcct cgtccaagtg cagccccgca cggtgggata
    82981 ataatcacca ccacgctaat cacgaccagg ctaatcactc gtagaagggc tgtcattttg
    83041 tcttttcttg catcttgttc gaaggaatgc ctaaagagtt accataggtt ttgtaaatag
    83101 tacaagatat tttttggccg atttttatga tgcgtttaat aatttcaata taaattgttg
    83161 ttttttaaaa ataaaatagt gagtgaaaaa aattcaattt cagtatgaat gttgttccct
    83221 tcctcgtagt tcatcctttg tccgctgcat ttttgcgagc atcattccac cgccagaaca
    83281 actcaacttc acttgcacaa ataaacagga atcagccact aaaattcacg aattagcggg
    83341 ctacagcagc gatatcttat gcacataatg gtgactcaag gagggcttat gtcactgtca
    83401 attgttcata cccgcgcagc cctgggagta aatgcgcccc cgatcactgt tgaggtacat
    83461 atcagtaaag gtctacccgg cttaacgatg gtgggcttac cagaaacaac ggtaaaagaa
    83521 gctcgcgatc gcgtgcgcag cgccattatc aatagcggat atgaatatcc ggcgaaaaaa
    83581 atcaccatca acctggctcc agccgatctg ccaaaagaag ggggacgata tgatttacct
    83641 atcgccattg cgttgctggc ggcctcagaa cagcttacag ccaataagtt agatgaatat
    83701 gaattagtcg gagaactggc tcttacaggc gctctgcgtg gcgttcccgg tgcaatctcc
    83761 agtgcaactg aagctattaa gtcgggcaga aaaattatcg tcgcgaaaga taacgaagat
    83821 gaagtggggc taattaacgg tgaaggatgc ctgatagccg atcatctgca agctgtctgt
    83881 gcgtttctgg aaggtaagca cgctctcgaa cgcccgaaac caactgatgc agtatcccgg
    83941 gcgctacaac atgatctcag tgatgttgtc ggtcaggaac aaggaaagcg aggactggaa
    84001 attaccgccg ctggcgggca caacctttta ctgattgggc cgccgggaac aggtaaaaca
    84061 atgctcgcca gccgtattaa tggcctgttg ccagatttaa gcaatgaaga ggcactggag
    84121 agcgctgcga tattaagtct ggtaaatgct gaatcagtac aaaaacaatg gcggcagcgc
    84181 ccgttccgct cacctcatca cagtgcgtcg ttaactgcga tggtgggcgg tggtgcaatt
    84241 ccagggccag gtgaaatttc gttggcgcat aacggcgtgc tttttcttga tgagctacct
    84301 gaatttgaac ggcgtacact ggatgccttg cgagagccga ttgaatccgg gcagatccat
    84361 ctttcacgca cacgagcaaa aataacctat ccagcccgtt tccagcttgt tgcggcgatg
    84421 aatcccagcc ctaccggaca ttatcaggga aaccataacc gctgcacgcc agaacagaca
    84481 ttacgttatc tcaaccggct ctcggggccc tttctcgacc gcttcgatct ctcactggag
    84541 atcccattac caccccccgg cattttgagt aaaacggtag tgccgggaga aagcagcgcc
    84601 accgttaaac aacgcgtaat ggccgccaga gagcgccaat ttaagcggca gaataaactg
    84661 aatgcctggc tggatagtcc ggaaatacgc caattctgca aacttgagag cgaagatgcg
    84721 atgtggttgg aaggaacact gatccatctg gggttatcga ttcgtgcctg gcagcggtta
    84781 ttgaaagttg cacgaaccat tgctgatatt gatcagtctg acattatcac acgtcagcat
    84841 ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc tcatccatct gcaaaaacta
    84901 ctgacataaa aaaagggcat ttcgcccttt ttattaatca tcagaatcgg tgtagtcttc
    84961 agcaccttca acctgcggtt taccgccgga aagggtgtga aaacgttttg gacgcttaat
    85021 acgcgtcata tacttggacc acacgcgttc tgcttctgtc actggctcac gttcgccacg
    85081 gcatactgct acgaagagtt tctcttcctc ggtaaccggc tcgcgtttgc caagatccaa
    85141 ctcattgaag gcataaccat gacgctcaag cagttgtgcc tctttgatgg tgaaatcacc
    85201 atgacgagag aatccacgtg gataatgttt attgtcgaaa tatcgattag tcgtcgtaaa
    85261 gctttccgcc atcctgcacg ctcctaattc tttgaccgag ctagttatgg cgcggagtat
    85321 tagttacgct tgacagagtg taaaacaaaa catttaaatc ataacgacaa ataattttgc
    85381 ggagagcact gtggatacgg aattgttaaa aactttcctg gaagttagcc gaacgcgtca
    85441 ctttggtcga gcggctgaat cgctctatct gacccagtca gcagtgagct ttcgaatcag
    85501 acaactggaa aatcaactgg gtgtgaacct tttcacccgc cacagaaaca atatccgttt
    85561 aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg ctcatgagca cctggcaggc
    85621 cgcccgtaag gaggtggcgc atacctcacg acataacgag ttttctatcg gtgccagcgc
    85681 ctcgttgtgg gaatgtatgc ttaatcagtg gctgggacgt ttgtatcaaa atcaggatgc
    85741 ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg cagtctctgg taaaacagct
    85801 gcatgaacgc cagcttgatc ttcttattac cactgaagcg cccaaaatgg acgaatttag
    85861 tagtcagttg ctgggatatt tcactttagc gctttatacc agtgcccctt caaaactaaa
    85921 gggagatctt aattatctgc gacttgagtg ggggccagat tttcaacagc atgaggcagg
    85981 tttgatcggt gctgacgaag tgcccattct gacaaccagt tctgctgaac tggcacagca
    86041 acagattgcg atgcttaatg gttgcacctg gctacccgtc agctgggcgc gtaaaaaagg
    86101 cggcctgcat accgttgtcg atagcacaac actttcacgg ccgctttatg ccatatggct
    86161 gcaaaatagc gataaaaata cgttgattcg cgatcttttg aaaattaacg tgctggatga
    86221 agtgtattaa tatgaatggc tggcaaggat gccggtagaa ggatttactt cggagagggt
    86281 tatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggca ggggcggaga
    86341 gactcgaact cccaacaccc ggttttggag accggtgctc taccaattga actacgcccc
    86401 taattag
//
LOCUS       DAMZET010000004        84554 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.004, whole genome shotgun sequence.
ACCESSION   DAMZET010000004 DAMZET010000000
VERSION     DAMZET010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 84554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.004"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            66..181
                     /gene="rrf"
                     /locus_tag="POB34_000314"
     rRNA            66..181
                     /gene="rrf"
                     /locus_tag="POB34_000314"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(413..1171)
                     /locus_tag="POB34_000315"
     CDS             complement(413..1171)
                     /locus_tag="POB34_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDI5917326.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(1179..2282)
                     /locus_tag="POB34_000316"
     CDS             complement(1179..2282)
                     /locus_tag="POB34_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDI5917327.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2292..3473)
                     /locus_tag="POB34_000317"
     CDS             complement(2292..3473)
                     /locus_tag="POB34_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDI5917328.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3541..4566)
                     /locus_tag="POB34_000318"
     CDS             complement(3541..4566)
                     /locus_tag="POB34_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI5917329.1"
                     /translation="MKKMMIATLAAASVLLAFANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4997..5218)
                     /locus_tag="POB34_000319"
     CDS             complement(4997..5218)
                     /locus_tag="POB34_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917330.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5471..8574)
                     /gene="acrF"
                     /locus_tag="POB34_000320"
                     /pseudo
     CDS             complement(5471..8574)
                     /gene="acrF"
                     /locus_tag="POB34_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(8586..9743)
                     /gene="acrE"
                     /locus_tag="POB34_000321"
     CDS             complement(8586..9743)
                     /gene="acrE"
                     /locus_tag="POB34_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HDI5917331.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            10142..10804
                     /gene="acrS"
                     /locus_tag="POB34_000322"
     CDS             10142..10804
                     /gene="acrS"
                     /locus_tag="POB34_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HDI5917332.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(10807..10986)
                     /locus_tag="POB34_000323"
     CDS             complement(10807..10986)
                     /locus_tag="POB34_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HDI5917333.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(11070..11954)
                     /gene="yhdJ"
                     /locus_tag="POB34_000324"
     CDS             complement(11070..11954)
                     /gene="yhdJ"
                     /locus_tag="POB34_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDI5917334.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NRSKRCRVSEVDPDLIAK"
     gene            12242..13465
                     /locus_tag="POB34_000325"
     CDS             12242..13465
                     /locus_tag="POB34_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023332881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDI5917335.1"
                     /translation="MAGGTNKLSDTSLRKMLGRESPGDSFYADGDGLSVKVSRSGVLT
                     WYFTFRIGGRESTSQRIKLGNYPDLSLKAAREKREQCRAWLAEGKNPKHQLSVTTQET
                     LKPVTVRDALEYWIREYATHNRANVEKHIEQLNKHIFPYIGTYPLSMCETRHWLECFA
                     RVRNEAPVAAGYLLQMCKQALKFCRVHRYAVSNVLDDLTIDDVGRKQNKRDREHTRQE
                     LADIWRECSGLKFKPYYSALLRLLVVFGCRTQELRLSRVTEWDLNDWVWTVPKEHSKG
                     GEKILRPIPVDIRPFIKQLLEQHQSTGLLLGEIKKPEAVSQWGRMLYKRLGHSEPWTL
                     HDLRRTFSTTLNNMGIAPHVVEQLLGHALGGVMSVYNRSQYLPEKLDALNKWMQRLDV
                     ITSDISNVTILEVAR"
     gene            13462..14304
                     /locus_tag="POB34_000326"
     CDS             13462..14304
                     /locus_tag="POB34_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917336.1"
                     /translation="MIKKINSKRDLPKSFSLEKYDDLENMSDKDLFRQLYWRCDDLTI
                     KNTDCPDYGLQYGAKYPLNNNFGDPFGELKAEEWFLEKQKEYEYKTQPDLLKLSYGDG
                     IKPLMRFELAFLNKINADKGHWKGKPIVVDDDLVGDLFTADNGMFWAVMREPVNLLSD
                     VVENVMITVDLNNRDDLLIEAFTNLLPKWREELGIPEPDKPVSGDWESVRRKIIDYRI
                     IPLIDLMSWESATDSKISLGVLAVSLFPDGEKESFAIAQTVKPFLDKIMRSDSLDKIR
                     KELS"
     gene            14420..14629
                     /locus_tag="POB34_000327"
     CDS             14420..14629
                     /locus_tag="POB34_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HDI5917337.1"
                     /translation="MNNIKQSQQPAERVIREAECRQLTGICRTTRYMMEKEGSFPARR
                     KLGGRAVGWLLSEVTAWQQSRSKAA"
     gene            complement(15239..15430)
                     /locus_tag="POB34_000328"
     CDS             complement(15239..15430)
                     /locus_tag="POB34_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HDI5917338.1"
                     /translation="MSTIKRDKTPKGDGWSPTFQIRINPDLRQQLNDAAEREGVSLGN
                     WIKELARAELRRQGIEPKG"
     gene            15564..15776
                     /locus_tag="POB34_000329"
     CDS             15564..15776
                     /locus_tag="POB34_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HDI5917339.1"
                     /translation="MAELQHTQTRPEFTWRFISASERYPTAKPLVIYVNASSEQEARD
                     TMPGVNLIFAARLPFHAFQAMEVRHA"
     gene            15769..15954
                     /locus_tag="POB34_000330"
     CDS             15769..15954
                     /locus_tag="POB34_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917340.1"
                     /translation="MRELTNKSASIACELAALLMVVEECDVDQVERENLISLARRVSD
                     QLAASMVEQNSTGGLNG"
     gene            15947..16174
                     /locus_tag="POB34_000331"
     CDS             15947..16174
                     /locus_tag="POB34_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HDI5917341.1"
                     /translation="MDNFYTYRSKKDLLLLAQEVAALLSCAAYLATTRGEEERIHVMS
                     LTHLAQRLSDELANSLDISTFSDPESREAKS"
     gene            16171..16539
                     /locus_tag="POB34_000332"
     CDS             16171..16539
                     /locus_tag="POB34_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917342.1"
                     /translation="MISNVKFYELAKRVDLLVEKVLHLEAQVKSLTDSQGGEIPPGMT
                     PIATLAAEYGISTKKAEELAKNTGVMLVKLKSGGFVAPDEKFREAARLVLRSAKRKYG
                     SAYWFHPLIGKFQMSGGIPK"
     gene            16662..16946
                     /locus_tag="POB34_000333"
                     /pseudo
     CDS             16662..16946
                     /locus_tag="POB34_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229111.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
     gene            16948..18162
                     /locus_tag="POB34_000334"
     CDS             16948..18162
                     /locus_tag="POB34_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDI5917343.1"
                     /translation="MWWPFSRKKSEQRNLSIDDFLALSGVPNTGSGEYVSAGTAESLP
                     AVMNAVSVIAEAVATMPCYLYLVRNDKGREAREWLDSHPVDILLNEQPNSCQTPYQFK
                     RTMMRHCLLNGNAYAVIEWGRDGQPKSLHPYAPGCVVPERTGAHKYRYTITEPCTGTV
                     RTYLQEEVLHLRYASDDGFLGRSPVTICREALGLGLAQQRHGASIMKDGMMAAGIITS
                     GEWLDGVKGKQALDALERYKGAKNAGKTPILEGGMDYRQLGMSNQDAEWLASRRFSIE
                     DIARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKAALLVASPVP
                     GTRYLVEFDSADLLRATPTERYATYEKGIKNGIMNPNEAREREGMPPREGGDEFSQAW
                     KQTVEIKGEKDE"
     gene            18155..18457
                     /locus_tag="POB34_000335"
     CDS             18155..18457
                     /locus_tag="POB34_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDI5917344.1"
                     /translation="MSESRITLNEVMAHLRLDDDFYGEVELLQIYTDAALEACQKHIG
                     KRFEDGLEFTPAIRVGCLMYIAFLYENREAVSPVEHSELPMAISALWSVYRDVGVY"
     gene            18457..18897
                     /locus_tag="POB34_000336"
     CDS             18457..18897
                     /locus_tag="POB34_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDI5917345.1"
                     /translation="MPWQPLRRCTEPGCNKRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRALYLSKNPLCVRCLAKGIYTPALVVDHIIPINGGGDVLFWPEWNHQAL
                     CQTCHNRKTTREDPATKANRKAGMYREQEERAAHRNDWMYGDDD"
     gene            18881..19066
                     /locus_tag="POB34_000337"
     CDS             18881..19066
                     /locus_tag="POB34_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917346.1"
                     /translation="MAMMTEQEQTRLIRGLIRQRDTWKTQETEHKANRTGRTKRTTAK
                     RLTDRDREVMECFRNRW"
     gene            19186..19542
                     /locus_tag="POB34_000338"
     CDS             19186..19542
                     /locus_tag="POB34_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDI5917347.1"
                     /translation="MARPPKAPAYLDEIAVRQWKEKSRQLSGREDLTPADWSNLELYC
                     VNYSIYRKAVEDLATRGFSIVNSQGSESRNPALSAKADAERIMIKMASLLGFDPVSRR
                     RNPPETEEEDELDRLA"
     gene            19526..21187
                     /locus_tag="POB34_000339"
     CDS             19526..21187
                     /locus_tag="POB34_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDI5917348.1"
                     /translation="MTAWHEYAEGVKNGKITACKRLKQAVKRYFSDLENSLYTFDPEV
                     VERFIAFSRVCPHVKGAMRGSPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTVAAILANWFLVMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRKRVNI
                     QAHKVIHPKTNSLLKPLAAKAATIEGTNPSLAIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEMNESMFVLIYELDDESEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMLTKRFNIWCQGATPWMGNGAWAECAGT
                     FAEADLYGQECYAGLDLSSTSDISSVCYAFPVGKKIMLVSRHYLPEFQLQNPANKNRA
                     IYRQWVKAGWIRTTPGDCIDYDRIRDDIMADAENFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRKVIVHRGDPVLAWSMSNVVMQSDANANIKPNK
                     KKSSNKIDPSVAALMAFGTFQAEHEEFAFDMSDSHKERLAAFDGV"
     gene            21193..21474
                     /locus_tag="POB34_000340"
     CDS             21193..21474
                     /locus_tag="POB34_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917349.1"
                     /translation="MTETDLLKIIRRITGISQQPDEQATQPDSVIAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRSQQRRESAKNTYQWKKPERLRR"
     gene            complement(22256..22552)
                     /gene="fis"
                     /locus_tag="POB34_000341"
     CDS             complement(22256..22552)
                     /gene="fis"
                     /locus_tag="POB34_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HDI5917350.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(22578..23543)
                     /gene="dusB"
                     /locus_tag="POB34_000342"
     CDS             complement(22578..23543)
                     /gene="dusB"
                     /locus_tag="POB34_000342"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDI5917351.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(23872..24753)
                     /gene="prmA"
                     /locus_tag="POB34_000343"
     CDS             complement(23872..24753)
                     /gene="prmA"
                     /locus_tag="POB34_000343"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDI5917352.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(24765..26216)
                     /gene="panF"
                     /locus_tag="POB34_000344"
     CDS             complement(24765..26216)
                     /gene="panF"
                     /locus_tag="POB34_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HDI5917353.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLVAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(26206..26448)
                     /locus_tag="POB34_000345"
     CDS             complement(26206..26448)
                     /locus_tag="POB34_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HDI5917354.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEIACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(26557..27906)
                     /gene="accC"
                     /locus_tag="POB34_000346"
     CDS             complement(26557..27906)
                     /gene="accC"
                     /locus_tag="POB34_000346"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDI5917355.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(27917..28387)
                     /gene="accB"
                     /locus_tag="POB34_000347"
                     /gene_synonym="fabE"
     CDS             complement(27917..28387)
                     /gene="accB"
                     /locus_tag="POB34_000347"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDI5917356.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(29365..30339)
                     /locus_tag="POB34_000348"
     CDS             complement(29365..30339)
                     /locus_tag="POB34_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDI5917357.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEMLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            30491..32431
                     /gene="csrD"
                     /locus_tag="POB34_000349"
     CDS             30491..32431
                     /gene="csrD"
                     /locus_tag="POB34_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HDI5917358.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            32736..33779
                     /gene="mreB"
                     /locus_tag="POB34_000350"
     CDS             32736..33779
                     /gene="mreB"
                     /locus_tag="POB34_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HDI5917359.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            33845..34948
                     /gene="mreC"
                     /locus_tag="POB34_000351"
     CDS             33845..34948
                     /gene="mreC"
                     /locus_tag="POB34_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDI5917360.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            34948..35436
                     /gene="mreD"
                     /locus_tag="POB34_000352"
     CDS             34948..35436
                     /gene="mreD"
                     /locus_tag="POB34_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDI5917361.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            35445..36038
                     /gene="yhdE"
                     /locus_tag="POB34_000353"
     CDS             35445..36038
                     /gene="yhdE"
                     /locus_tag="POB34_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HDI5917362.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            36028..37497
                     /gene="rng"
                     /locus_tag="POB34_000354"
     CDS             36028..37497
                     /gene="rng"
                     /locus_tag="POB34_000354"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HDI5917363.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            37565..41365
                     /gene="yhdP"
                     /locus_tag="POB34_000355"
     CDS             37565..41365
                     /gene="yhdP"
                     /locus_tag="POB34_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HDI5917364.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGVRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            41521..42966
                     /gene="tldD"
                     /locus_tag="POB34_000356"
     CDS             41521..42966
                     /gene="tldD"
                     /locus_tag="POB34_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HDI5917365.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(43094..44023)
                     /gene="aaeR"
                     /locus_tag="POB34_000357"
     CDS             complement(43094..44023)
                     /gene="aaeR"
                     /locus_tag="POB34_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HDI5917366.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            44206..44409
                     /locus_tag="POB34_000358"
     CDS             44206..44409
                     /locus_tag="POB34_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HDI5917367.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            44417..45349
                     /gene="aaeA"
                     /locus_tag="POB34_000359"
     CDS             44417..45349
                     /gene="aaeA"
                     /locus_tag="POB34_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HDI5917368.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            45355..47322
                     /gene="aaeB"
                     /locus_tag="POB34_000360"
     CDS             45355..47322
                     /gene="aaeB"
                     /locus_tag="POB34_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HDI5917369.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            47414..47686
                     /locus_tag="POB34_000361"
     CDS             47414..47686
                     /locus_tag="POB34_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HDI5917370.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(47742..48005)
                     /gene="yhcN"
                     /locus_tag="POB34_000362"
     CDS             complement(47742..48005)
                     /gene="yhcN"
                     /locus_tag="POB34_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HDI5917371.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(48370..48840)
                     /gene="argR"
                     /locus_tag="POB34_000363"
     CDS             complement(48370..48840)
                     /gene="argR"
                     /locus_tag="POB34_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDI5917372.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            49275..50213
                     /gene="mdh"
                     /locus_tag="POB34_000364"
     CDS             49275..50213
                     /gene="mdh"
                     /locus_tag="POB34_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDI5917373.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(50275..51342)
                     /gene="degS"
                     /locus_tag="POB34_000365"
     CDS             complement(50275..51342)
                     /gene="degS"
                     /locus_tag="POB34_000365"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HDI5917374.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(51432..52799)
                     /gene="degQ"
                     /locus_tag="POB34_000366"
     CDS             complement(51432..52799)
                     /gene="degQ"
                     /locus_tag="POB34_000366"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HDI5917375.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(52953..53351)
                     /locus_tag="POB34_000367"
     CDS             complement(52953..53351)
                     /locus_tag="POB34_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HDI5917376.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            53545..54672
                     /gene="zapE"
                     /locus_tag="POB34_000368"
     CDS             53545..54672
                     /gene="zapE"
                     /locus_tag="POB34_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HDI5917377.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMMRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            54891..55319
                     /gene="rplM"
                     /locus_tag="POB34_000369"
     CDS             54891..55319
                     /gene="rplM"
                     /locus_tag="POB34_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDI5917378.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            55335..55727
                     /gene="rpsI"
                     /locus_tag="POB34_000370"
     CDS             55335..55727
                     /gene="rpsI"
                     /locus_tag="POB34_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDI5917379.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            56122..56760
                     /gene="sspA"
                     /locus_tag="POB34_000371"
     CDS             56122..56760
                     /gene="sspA"
                     /locus_tag="POB34_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HDI5917380.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            56766..57263
                     /gene="sspB"
                     /locus_tag="POB34_000372"
     CDS             56766..57263
                     /gene="sspB"
                     /locus_tag="POB34_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HDI5917381.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(57306..58673)
                     /gene="dcuC"
                     /locus_tag="POB34_000373"
     CDS             complement(57306..58673)
                     /gene="dcuC"
                     /locus_tag="POB34_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDI5917382.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKALDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            59053..59844
                     /gene="nanR"
                     /locus_tag="POB34_000374"
     CDS             59053..59844
                     /gene="nanR"
                     /locus_tag="POB34_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HDI5917383.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            59966..60859
                     /gene="nanA"
                     /locus_tag="POB34_000375"
     CDS             59966..60859
                     /gene="nanA"
                     /locus_tag="POB34_000375"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDI5917384.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            60968..62458
                     /gene="nanT"
                     /locus_tag="POB34_000376"
     CDS             60968..62458
                     /gene="nanT"
                     /locus_tag="POB34_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HDI5917385.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            62506..63195
                     /locus_tag="POB34_000377"
     CDS             62506..63195
                     /locus_tag="POB34_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDI5917386.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            63192..64067
                     /gene="nanK"
                     /locus_tag="POB34_000378"
     CDS             63192..64067
                     /gene="nanK"
                     /locus_tag="POB34_000378"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDI5917387.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            64064..64528
                     /locus_tag="POB34_000379"
     CDS             64064..64528
                     /locus_tag="POB34_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDI5917388.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(64588..65715)
                     /locus_tag="POB34_000380"
     CDS             complement(64588..65715)
                     /locus_tag="POB34_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDI5917389.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHTPTETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(65899..67317)
                     /gene="gltD"
                     /locus_tag="POB34_000381"
     CDS             complement(65899..67317)
                     /gene="gltD"
                     /locus_tag="POB34_000381"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HDI5917390.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(67330..71790)
                     /gene="gltB"
                     /locus_tag="POB34_000382"
     CDS             complement(67330..71790)
                     /gene="gltB"
                     /locus_tag="POB34_000382"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HDI5917391.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPHIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            72465..73394
                     /locus_tag="POB34_000383"
     CDS             72465..73394
                     /locus_tag="POB34_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDI5917392.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
     gene            73490..75826
                     /gene="arcB"
                     /locus_tag="POB34_000384"
     CDS             73490..75826
                     /gene="arcB"
                     /locus_tag="POB34_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HDI5917393.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            76056..76709
                     /gene="elbB"
                     /locus_tag="POB34_000385"
     CDS             76056..76709
                     /gene="elbB"
                     /locus_tag="POB34_000385"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HDI5917394.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            76706..77434
                     /gene="mtgA"
                     /locus_tag="POB34_000386"
     CDS             76706..77434
                     /gene="mtgA"
                     /locus_tag="POB34_000386"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HDI5917395.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(77431..78063)
                     /gene="yrbL"
                     /locus_tag="POB34_000387"
     CDS             complement(77431..78063)
                     /gene="yrbL"
                     /locus_tag="POB34_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HDI5917396.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(78277..78549)
                     /gene="npr"
                     /locus_tag="POB34_000388"
     CDS             complement(78277..78549)
                     /gene="npr"
                     /locus_tag="POB34_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HDI5917397.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            complement(78546..79400)
                     /gene="rapZ"
                     /locus_tag="POB34_000389"
     CDS             complement(78546..79400)
                     /gene="rapZ"
                     /locus_tag="POB34_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDI5917398.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYRVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            complement(79446..79937)
                     /gene="ptsN"
                     /locus_tag="POB34_000390"
     CDS             complement(79446..79937)
                     /gene="ptsN"
                     /locus_tag="POB34_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HDI5917399.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            complement(80055..80342)
                     /gene="hpf"
                     /locus_tag="POB34_000391"
     CDS             complement(80055..80342)
                     /gene="hpf"
                     /locus_tag="POB34_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HDI5917400.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            complement(80365..81798)
                     /gene="rpoN"
                     /locus_tag="POB34_000392"
     CDS             complement(80365..81798)
                     /gene="rpoN"
                     /locus_tag="POB34_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDI5917401.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            complement(81846..82571)
                     /gene="lptB"
                     /locus_tag="POB34_000393"
     CDS             complement(81846..82571)
                     /gene="lptB"
                     /locus_tag="POB34_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HDI5917402.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            complement(82578..83135)
                     /gene="lptA"
                     /locus_tag="POB34_000394"
     CDS             complement(82578..83135)
                     /gene="lptA"
                     /locus_tag="POB34_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HDI5917403.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            complement(83104..83679)
                     /gene="lptC"
                     /locus_tag="POB34_000395"
     CDS             complement(83104..83679)
                     /gene="lptC"
                     /locus_tag="POB34_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HDI5917404.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            complement(83676..84242)
                     /gene="kdsC"
                     /locus_tag="POB34_000396"
     CDS             complement(83676..84242)
                     /gene="kdsC"
                     /locus_tag="POB34_000396"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HDI5917405.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            complement(84263..>84554)
                     /locus_tag="POB34_000397"
     CDS             complement(84263..>84554)
                     /locus_tag="POB34_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDI5917406.1"
                     /translation="TRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
BASE COUNT        20665 a        21129 c        22154 g        20606 t
ORIGIN      
        1 cagttcgact ctgggtatca gcaccacttt ttaggttaaa gttcggcaga ttagaaaaga
       61 atttgtctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga
      121 aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccaga
      181 catcaaataa aacaaaaggc tcagtcggaa gactgggcct tttgttttat ttgatgtttg
      241 tcggtgaaca ctctcccaac caggacaaat ccgccgggaa cggatttgaa cgttgcgaag
      301 caacggcccg gaggctggcg ggcaggacgc ccgccataaa ctgccagaca tcaaatcaag
      361 caaaaaggcc atccgaaagg atggcctttt tgcttttcga actaacattc aattaatgga
      421 ttacctgcga taaaaatgcc ctcgtacgct ctgatttagg atgcgtaaaa aattcatcag
      481 gtgcagcttg ctccactatt tccccacgat ccataaaaat cacccggtca gcgacggttc
      541 gcgcaaaccc catctcatgc gttacacaca acattgtcat acccgattgc gccagcccaa
      601 tcatcgtatc cagcacctct ttcaccatct ccggatcgag cgccgacgtt ggctcatcaa
      661 acaacataat tttcggcttc atacacagcg aacgcgcaat ggcaacgcgt tgctgctgac
      721 caccagaaat ttgtccggga aacttatgcg catgttcggc aattctcacc cgctcaaggt
      781 aatgcatcgc cagagcttca gcctctttct ttggcatctt gcgtacccaa atcggtgcca
      841 gggtacagtt ctgtagaacg gtcagatgag ggaagagatt gaaatgctga aagaccattc
      901 ccacttcctg ccggacgcgc tcaatattgc ggatatcttc attaagttcg atgccatcta
      961 ccacgatccg tccctgttga tgttcttcca gatgattaat acaacgaatg gttgtcgatt
     1021 taccggaacc tgaagggcca cacagaacga tccgttctcc cggttgcacg gttaaattta
     1081 tatttttcaa aacatggaat tgtccatacc atttattgac gttttccagc gtaatcatcg
     1141 cgttagcagg ttgcagtaaa atttggctca tagtgtcctc aatgcggtgt acgcccggtg
     1201 ttaaaacgtt tttccagatg ctggctatag cgcgacatgc tgaaacagaa gatccagtag
     1261 atcagcgcgg cgaaaacata cccttccgtc gacataccca accaggtggg atcaacggtt
     1321 gcctgctgaa cgctactgaa aagatcgaac aacccgataa tgatcaccag gctggtatct
     1381 ttgaagagtg cgatgatggt atttaccagc ccaggaatta ccagcttcaa cgcctgtggc
     1441 agaataacca gcccctgagt tttccagtaa cccaacgcca gcgactctgc cgcttcatac
     1501 tgccctttag gcagtgcctg taatccgcct cgcacgactt ccgcaacata tgctgactga
     1561 aacaggatca cgccaaccag cgcgcggatc agtttgtcga tactggtgcc ttctgccata
     1621 aacaacggca gcatgaccga agacataaac aatacggtaa tcagcggtac gccgcgccag
     1681 aactcgataa aaatgaccga taagatacgc acaatcggca tatgggagcg gcgacctaac
     1741 gccagtaaga tcccccacgg tagcgccccc gcaatcccaa cggatgcaat aattaaagtt
     1801 agcgtcagcc cgccccattg ccgggtttca acccgctcca gaccaaaaaa accgccatac
     1861 atcagccacc agacaatcag tgggtaaatc accgcccaga ccgcaatata gcgaccgcga
     1921 tgcgggagta ttttccagaa cattggtgcg attgacacaa ggccaatcag taatgccagg
     1981 ttaattcgcc agcgttggtc gtgtgggtaa agtccataca taaactgacc aaatcgttcg
     2041 tggatgaaga cccaacaggc accggctttt gtgcagtcgg cacgcgttga gccaacccaa
     2101 ttagcctgca aaaatgccca gttcagcaac ggtgggatca gttcccacat caaccatatg
     2161 cagccaatag tcagcaggct attgctccag ctggagaaca gattttttcg cacccacacc
     2221 atcgcgcggc ttgagttatg gctcgctggg cgcgagggct gagacagcaa tacttttgtc
     2281 atcatgactc cttagcgttc aacgatcgcg atgcggcggt tatagatatt catcagcagc
     2341 gagatagtca ggctgataat cagatagacc gacatggtca tggctatcgt ctcgatggct
     2401 tgccccgtct ggttcagcac ggtgccagca aacagcgaaa ccatatcggg atagccaata
     2461 gcggcggcaa gagaggagtt tttgacgatg ttgagatact ggctggttaa cggcggaata
     2521 atcactcgca gtgcctgggg aataatgacc tggcgtagcg taaccgggtt gggtaatccc
     2581 agtgaccgag ccgcttcatg ttgaccataa ggcactgcct ggatcccagc gcggataatc
     2641 tcggcgataa atgcagatgt ataaaccgaa agtgccagcg ttaaggctgc cagttcagga
     2701 attaaaacca tcccgccgcg gaagttaaag cctcgtaggg ctgggacatc ccagtgtagt
     2761 gctgcgccaa aaagccattg cgccagtaaa ggcaaaccaa tgatcaacac tgcggcgatc
     2821 ggccaggtgc ggcgcagttg tccggtcttt atctggtatg ttttattaaa gcggaatagc
     2881 ccaacagaaa ggactatagc cataacaaca gccaggataa acgcaataaa tccgtctccc
     2941 agctgcggtg acggaatata aagcccacga ttgctcaaaa aggccagatc aaacgcgctc
     3001 acagcctggc gtggtccggg caaattgcgc aacacggcaa agtaccagaa gaagatttgc
     3061 agcagcgggg gaatattacg gaagatctca atataaattg tggaaagctt tcgtagcagc
     3121 cagttatccg aaagtctcgc cagaccgata aagaagccca aaacagaagc gaacacaata
     3181 cacaatgcag aaaccagtag cgtattgagt aagccgacaa taaaaacgcg tccgtaggtg
     3241 tcgccctgct ggtaatcgat caaatgctgg acaataccga agccagcgcc gcgatccaga
     3301 aaggcaaaac ctgaagtaat gccacgatta ttgagattcg ttacagtgtt gtgaaataac
     3361 caaccaacaa tgccgacaac agcaacaacg gcaaggatct ggaataacca ggcacgaacc
     3421 gtagggttgg caaaggagag tgagccttta acggttgagc ggcgatgaga catagaaacc
     3481 tcggaaagca taacgctgaa gagagcgccg ccgtcgcgac gctcgttacc tcacaccgac
     3541 ttaacgcacg ggcggtgcgt actgaatacc gccgttattc cagagattat tttgcccacg
     3601 tttaattttc agcgggcttt ctgaacctac gttacgctca aaaatttccg agtagttacc
     3661 cacctgtttg atgatgttat aggcccattt attatccaac ttcagatcct tgccgtaatc
     3721 gccttcttta cccagcagat gtgccatatc aggcgttgct ggattagccg ctttttcatc
     3781 aacgttctgg gaattgatgc ccatctcttc agcattcagc atggcgaaaa gcgtccagcg
     3841 tacaatcgag aaccattcat catcgccacg acgaactacc ggcccaagcg gttctttaga
     3901 gataacctcc ggtaagacaa tccattcagc tgggttgctt aatttgatgc gcagggcata
     3961 cagttgcgac tgatccgagg ccagcgtatc gcagcgacca gattccagtg ccttcgctga
     4021 ttcgtcagag cgatcgaaag tcactggtgt gtacttcata ttgtttgcct tgaaatagtc
     4081 ggcaacgttg agttcggtat cagtacccgc ctgaatacag acggtagcgc catccagttc
     4141 tttcgcgctt tttagccccg ctttatcgtg cgtcaggaag ccaatgccgt cgtaataagt
     4201 gacgccagta aatgccattc ccatcccggc atcgcgagat gaagtccagg tcgtattacg
     4261 ggagagcaaa tccacttccc ctgactgtaa agccgtgaag cgttcttttg ctgtgagtgg
     4321 ggtatatttc actttcgtgt cgtcaccaaa tacagcagcg gcaacaccac gacaaacatc
     4381 aacatcaata cctgaaaact taccgtctgc atcggcataa gagaacccag gtaatccatc
     4441 actgatcccg cattgcacaa aacctttttt ctgaacggca tcaagcgtcg cgccagcatg
     4501 cgcctgattt gcaaaggcaa gcagcacgct ggcggcagcc agtgtggcta tcatcatctt
     4561 tttcataatg cttcctgtga ggcgaaaatt atcgttatag gacgtttggg aaacgtcttt
     4621 tacctgtctg tggccatcct cttataagca aaagtagtgc cagcttttgc gcccgatttc
     4681 gcgtcgcaat aagctgagtg tagacaggaa ataataaaaa cagcgccccg caatggtgcg
     4741 aaaatgaaac atgcgccaca ttttggcgca aaatatttac gcgtgtttta ctaagcagaa
     4801 taatgattgt tagcgtgagt atatttctgg tgggaagtgt gaaagttaaa ttgatgcgtt
     4861 atttcgaaag cagcgaaata acgcgatata acatgctaaa tcagcagaga attagccagc
     4921 agaatatagc aatattttat cttatgtaaa gatactgtat cgttaaacag agcgcgaaac
     4981 tccgcgccct gttcagttat ttcgacagag cgatgatccc catcgtcagg ccagcaacag
     5041 ccgctgcccc gccagcaata gccccagccg tttcccagtt atcctgagtc gtgcctttca
     5101 tacaggtatt ggtgtgaacc agacgaccct gatcgtcata aaccggaacg cagggcgaat
     5161 cgtgagcaca gcccgccagc aatgcggtga gcaatgccac aggaattaat cttttcatgg
     5221 tgttacctca gtaataaacg ctaacgctta ataagttaac tctcttatta acgagctgaa
     5281 tgccatttta tcaccggagg ttataaatct tgcggaatga ataatgacaa attatgtgaa
     5341 ttgtaaaaat agtgaaggga gcgggaacta tagaaagctt ttaaagagtg agcagaagaa
     5401 tatcttatta atttttatgg gtttcactgg aaataataaa ggcacccgaa agcgccttta
     5461 tgtttctgat ttatccttta aagcaacggc ggatcaccac aaagaacacc ggtacgaaga
     5521 agattgccag caacgttgca gagaccattc ctcccattac cccgataccc acagcgttct
     5581 gcgcgccact gccggcaccg ttactgatag ctagcggtaa tacgccgaga ataaaggcga
     5641 gagaggtcat caggatagga cgcagacgca tacgtactgc cattagtgtc gcttcaacaa
     5701 cacctttacc ctctttctcc atgagatctt tagcgaactc aacaatcaaa atagcgtttt
     5761 tggccgacaa gccaattgtc gttagcaagc ccaccataaa gtagacgtca tttttttgat
     5821 taaagagtgt cgccgccagc agcacgccga caatccctaa cggcacgacc aacataaccg
     5881 agacaggaat tgaccagctt tcatagagtg cagcaaggca caggaaaaca accacaaagg
     5941 aaattgctac cagagcggga gcctggtttc ccgataagcg ttcctgatac gacatacccg
     6001 tccagtcata accaatgcca gcaggtaatt ttgacgcaag gttttccatc aacgccatgg
     6061 catcgccgga actggttcct ggcgcggctt ccccctgaat ctccattgac ggcagaccgt
     6121 tgtagcgttc cagtcgcgga gagccataca cccaatgtga agtggtaaag gccgagaatg
     6181 gcaccatttc gccgttggcg ctgcggacat aaagtttatc gacatcttct ggcagcatac
     6241 ggaatttggc atccgcctga acatacacct ttttcacgcg accacggtcg atgaagtcgt
     6301 taacgtaagt cccacccaac gccgttgaaa tggtctgatt gatgtcagaa agtgaaacac
     6361 ctaatgcctg cgccttttcc tggtcaactt ccagtttaaa ctgcgcggtg tcttccaggc
     6421 cattaggacg cacgctgact aagctggcag gatgttgtgc cgccatacca agcaactggt
     6481 tacgggcctg ggttagggca tcgtgaccca gcccagcctg atcaattaac tcaaagtcga
     6541 aacccgttgc cgtgcccagt tcaacaatgg ctggcatatt gaatggaatg acaaaaccgt
     6601 cgcggatctt gccaaattcc attttggcac gatggattac cgcttccgca ctgttttcat
     6661 caccattacg ctcttcccac ggtttcagac tgacgaaggc cataccggca tttgtgcctg
     6721 gccgctgaag ctaaagccgt taaccgtaaa gacactttca acgttcgctt tctcgttctt
     6781 cagatagtaa tccgtcactt gatccaacac tttttgcgtc cgctcttgcg tcgcgccagc
     6841 gggtaactga atcatggtca gaaagacacc ctgatcctct tcaggtaaga aggaagacgg
     6901 aagacgtaaa aacaacacca ccattcctgc aacaatcagc gcatagatca gtaaatatcg
     6961 tcctgtggaa ccgaggattt tgccgacgct gttggtgtag tggttaacgc tatgatcgaa
     7021 ggtagtatta aaccagccga agaaaccgcc cttattttcg tgatgctcag cagagacggg
     7081 tttaagcagc gttgcacata acgcaggggt aagaatcaat gccaccagaa cagaaagcgc
     7141 cattgccgaa acgatggtga tagagaactg gcgataaatt gccccagtag aaccgccgaa
     7201 gaatgccatc ggaataaata ccgctgacag caccatcgcg atacccacca gtgcgccctg
     7261 gatttgcgac attgattttt ccgtcgcttc ttttggcggg agcttatcct ccatcatcac
     7321 gcgctcgacg ttctccacca ccactatcgc atcatcgacg agcagcccga tggcaagcac
     7381 catcccgaac atcgttagtg tgttgatgga gtaaccaaaa gcggcgagga tggcaaacgt
     7441 ccctaacaac accacgggta ccgcaatggt ggggatcagc gttgctcgca tattctgcaa
     7501 gaacagatac atcaccagga acaccagcat aatggcttcg aacagcgttt ttaccacttc
     7561 gtgaatagaa agctggacga atggcgtggt gtcataaggg tagagaacct tcattccctg
     7621 cgggaagaat ggctgtaatt ccgccagttt tgccttaatg gctttcgcgg tatcgagagc
     7681 attcgcgccg gttgccagct taatccccag gcccgccgcc ggttttccgt tgatacgagc
     7741 gataacgtta tagttttcac cgccaagttc aacccgtgcg acatctttca ggcgtaccac
     7801 cgagccgtca ctgtttacgc gcagggtcac tttgccgaat tcttccggat tttttaaccg
     7861 cgtttgagca ataatcgagg cattcaactg ttgccctggt aacgctggcg ttccgcccaa
     7921 ctgtccggca gcgatctgat cgttctgtac cttcaactgg ttaatcacat caaccggtgt
     7981 cagtttatat ttgtttagca gatcggcgtc cagccagata cgcatcgcat actgtgcgcc
     8041 gaaaagctgt acgtcaccga cgccattcag acggctaagc gtatctttaa cgttagaggc
     8101 cacatagtcc gagatatcgt cctgtgtggt gtctgggtta tcagagacaa agcccgccac
     8161 catcaaatag ctgctactgg acttttcaac actgatcccc tgctgctgaa cctcctgcgg
     8221 cagcaacggc gtggcgagct ggagtttgtt ctgcacctgc acttgcgcga tatcaggatc
     8281 ggtcccggac tggaaggtaa gggtaattgt cacgctaccg gcggaatcgc tggtggagga
     8341 catatacatc aggttatcga taccattcat attctgttcg ataacctgcg tcaccgtatc
     8401 ctgcacggtc tgcgcatcag cacctggata gtttgccgaa acagaaaccg caggcggtgc
     8461 aatggttgga tactgagcga cgggcaattg taggatcgcc agtgcgcccg ccatcatcag
     8521 aataatggcc agcacccatg caaatatcgg tcgtcgaata aaaaagtttg ccatgtcaga
     8581 ttaccttact tcgatgcagt atctgcgggg gtatcggtag tggctttaac ctgctctccc
     8641 ggacgcgctt tttgcaggcc gctgacaatg acttgatcgc cagatttcag accttcgcta
     8701 atcaaccatt tatcgccaat cgcctgactg gcaacgacag ggcgcgcttc cacctgactt
     8761 ttatcgttaa caatcagcac ggttgcatca ccacgcggtg tgcggctaac gccttgttgc
     8821 gggataagaa tggcgtcagg ttggacgcct tcatcaatcc gtgcacgcac aaacataccc
     8881 ggcaaaagcg tatgttgcgg gttagggaag acagcacgta gggttatgga gccggtgctt
     8941 tcatcaacgg tcacatcaga gaattgcagc gtacctttca ggggataggt ttgaccgttt
     9001 tccatgacca actctacgtt gctggtggcg ttttccttat gcaaatttcc ttgctctacg
     9061 gattgcttca gcctcataaa atcgttgctg gattgggtca catcaacgta gataggatcg
     9121 agctgctgga cagtcgccag ttcagtcgtt tgcccattag tgacaagagc gccttcggtc
     9181 acggtcgatt tgccgatacg tccgctaatt ggcgcagtca ctttggtata agcgagattg
     9241 atgcgagcgc tttcgactgt ggcttttgcg gcaatcacgg cggcatcggc ctggcgagca
     9301 tcagcaatgg cctggtcgta ctcctgctgg ctgatgtatt tcgtacccac gagcggaacg
     9361 taacgtttta ccgtcaaatg cgcgatggcg gcggcggctt cacttttcgc cagttcgcct
     9421 ttcgcgctgt cataatttgc ctgataggtc gcgggatcga tctggtacag ggactggcct
     9481 gcttgtacat cgctgccttc agtgaaatta cgattcagta cgatcccgct aacctgtggg
     9541 cgaacttcgg ctatacggta agcattggtg cggcctggta attcagtctt aacttctaac
     9601 ggggccgttt tcacaatatg aacggtaacc tgcggttcac cgacgtgagc tttctcttct
     9661 cccttatcgt tacaaccggc gattaaagcc gcggagatca gaataaagga gggcaggagg
     9721 aaaaacctgg catgtttcgt cattactatt cctcaaaaaa ccaaaagcgc gttatttacc
     9781 cagaaaggca acgcgttaac tcgcagaaag aaaaatacag tttgctatcc tacaaattat
     9841 cattcgtcga tgtaaggaat agttatgaat actggcgtct caaggcacat aaacacaaaa
     9901 aaagattaat attctactgt tttattttga cgcgggttga aagaggcaga attaaaacct
     9961 cgtaaattga aatatatatt catgtggtga atgtatttta ggtaaataat atatattatt
    10021 tatttgacga tttatagcaa ctcaatatta gcctctcgta taaatacaca ttaggtgata
    10081 gattaacctt cgctattttc tcactctgtg tcgaatatat ttatttcctg aataattaat
    10141 catggcaaaa agaaccaaag ccgaagctct gaagacccgg caagaactga ttgaaactgc
    10201 catcgcccag tttgcgcagc atggcgtaag caagacgaca ctcaacgaca ttgccgacgc
    10261 tgctaacgtt acgcgtggcg ctatctactg gcacttcgaa aacaagactc aactgtttaa
    10321 tgagatgtgg ttgcaacagc cttcattgcg ggagttaatc caggaacact tgacggctgg
    10381 attagagcat gacccgtttc aacaattgcg tgaaaaattg attgtcggct tgcaatatat
    10441 tgccaaaatt ccccgccagc aggcgttgct gaaaatctta tatcacaaat gtgaatttaa
    10501 tgatgagatg ctggccgagg gagtgatacg cgaaaagatg ggctttaatc cgcagactct
    10561 ccgcgaagta ttgcaggcgt gtcagcaaca aggttgtgta gcaaataacc tcgatttaga
    10621 tgttgtgatg attattattg atggtgcctt cagcggaatt gttcaaaact ggttaatgaa
    10681 tatggcgggt tatgatcttt ataaacaagc acccgcgctg gtcgataacg tattaagaat
    10741 gttcatgcca gatgaaaaca taacgaaatt aattcatcaa acgaatgaat taagtgtcat
    10801 gtaaattcac attctgtcct gaaaattcag ttcgtcacag ttaaccagtt ttagtggatt
    10861 aactgagttc atattgcggc atttgtctgt ttctgttgcc agcagttcaa tccactgcat
    10921 cagtaaagta tcaaacagaa aaacgaagac agcgaaaacg acgagccacc aatacttgcg
    10981 aatcattacc tgagcctgtg catgtttaac caagtctggc aggaaatata cacgaaatga
    11041 ataatctgaa aaccaggctt acacttcctt tactttgcaa tgagatcggg gtcaacttcg
    11101 ctaacccggc atcgttttga ccggttgccc gtctttcttt ttttcacttt cgccagttct
    11161 tccgcggagt aatgcgacgc gacatccagc cgtcgaagcc ccattttgat gtactcgctg
    11221 ttgatctcaa taccaatgaa ttttcgtccg ctggcgatgg ctacggcacc ggtagtaaag
    11281 ctaccggcaa acgggtcgag aacgatatcg cctgggttgg aagaggcgag aataatgcgt
    11341 ttcagtaagg cttccggttt ttgcgtcggg tggttctcat attcatccat taaataacgc
    11401 acgcgcggaa aatcccagac gttacccggt actttttgat gattgtatgg ctgtggagga
    11461 tttttgcgat aatcgatcaa cgcgcgctgc gatccggttt tggcttctac cagaatagca
    11521 tcaccgttga atgtgtagtt ctttgcgtct ttcaccatca tcaggatggg ttcgtacatg
    11581 gagccgtagt gttttttcgc ctgtactcca gaactgtcat atgaccagac gatgcgactt
    11641 ttgatggtaa aaagcttgcg gcactggaga tcgataaagg gcatgttttc cgtactgttc
    11701 ataatgtaca tgctgccctg ctttttcaga acgcggtggc actctgcaat cacttcaaac
    11761 agccagtcga taaacagatc ttctttccag gcttcgatca gaccatcaaa atttttaccg
    11821 atgttatacg gtgggtcggc aaagatcaga tcgacgcttt cagtaggtag ctttttaagt
    11881 tcggcaaggg catcaccgtg aataatggtc ttagcttcat taccaaaccg ggtcggttca
    11941 catcctgttc tcatggtcac tccctttgtg acacctataa aaaaggcgct tccccatgcc
    12001 gagtagcgcc tttttaatca agcatttagc taacctgaat tagttcatgc cgtattttca
    12061 aaacctacgt tttttaccgt tcggatatgt ttgctaatgt tcatctatga tactgatata
    12121 aaacaataaa attgatttat cggtttgcct ttgttcattg ccgttcatag agtaacattg
    12181 atatgtgcag tcagtggtgc agtcagtttg gcgtaccaaa tgatatgaac tgaggttttc
    12241 aatggctggt ggaacgaaca aattaagcga tacatcgctt cgtaaaatgc tgggaaggga
    12301 gagccccgga gacagcttct atgctgatgg cgatgggcta agtgtgaagg tgtccagatc
    12361 gggcgtgttg acttggtatt tcactttccg cataggtggc cgggaatcaa catctcagcg
    12421 tataaagctg ggtaattatc cagacctttc actcaaagca gctcgtgaga aacgagagca
    12481 gtgtcgcgca tggctcgcag agggaaagaa tccaaagcac cagttgagcg ttacgactca
    12541 ggaaacgtta aagccggtga cagtcaggga tgcccttgaa tactggatca gggaatatgc
    12601 cacccataac cgcgctaatg ttgaaaagca cattgagcag ctcaataagc atatctttcc
    12661 ttatataggt acttatccgc tttctatgtg tgaaactcgc cactggctgg aatgtttcgc
    12721 cagggtaagg aacgaagcgc ctgtagcggc tggctatctt ttgcagatgt gtaagcaagc
    12781 actcaagttc tgccgggttc atcggtatgc ggtgagtaac gtcctggacg atctgaccat
    12841 tgacgatgtt ggacgtaaac agaataagcg ggacagggag catacccggc aagaacttgc
    12901 tgatatatgg cgagaatgct caggcctgaa atttaagccc tactactcag ctctattacg
    12961 tctgctggtg gtgtttggct gccgaactca ggagctgaga ttatctcgcg taaccgaatg
    13021 ggatctaaat gattgggttt ggacggtacc taaagagcac agcaaaggtg gtgagaaaat
    13081 actacggcct attcccgtag atattcgccc atttatcaaa cagcttttag agcagcacca
    13141 aagcacaggg ctattgctgg gagagataaa gaaaccggaa gctgtcagtc agtggggaag
    13201 gatgttgtat aagcgtctgg gccactctga accgtggacg cttcacgatt tacgccgtac
    13261 attttccacg actctgaaca acatgggaat tgctccgcat gttgtcgagc agcttctggg
    13321 ccatgcgctg ggcggtgtca tgtccgtata taaccgcagt cagtatttac ctgaaaagct
    13381 ggatgcactg aataaatgga tgcagagatt agatgttatt acctctgata tctcaaatgt
    13441 tactattctt gaggtcgcaa gatgataaaa aaaattaaca gtaaaagaga cttaccaaag
    13501 tcattcagct tagaaaaata tgatgacctt gaaaatatgt ctgataagga tttgtttcgt
    13561 cagctttatt ggcgatgcga tgatttaaca ataaaaaata cagactgccc tgactatggt
    13621 ttgcaatatg gtgctaaata cccgcttaat aataattttg gtgatccatt cggggaactc
    13681 aaagcagaag aatggtttct tgaaaagcaa aaggaatacg agtacaagac tcagcctgat
    13741 ttgctgaaat taagttacgg cgatggtata aaaccattaa tgcgatttga gttggcattt
    13801 ttaaataaaa tcaatgctga taaaggtcac tggaaaggaa agccaatagt agttgacgat
    13861 gatttggtgg gggatttgtt tacagcagat aacggtatgt tttgggctgt catgcgtgag
    13921 ccagtaaact tactttctga tgttgtggaa aacgtgatga ttactgttga cttaaacaac
    13981 agagatgatc ttttgattga agcattcacg aacctcctcc caaaatggcg cgaagaactc
    14041 ggaatacctg aaccagataa gccagtctca ggggactggg agagcgtcag acggaagata
    14101 attgactaca gaataattcc acttattgat ttgatgtcat gggaaagcgc taccgacagc
    14161 aaaatatccc ttggcgtact cgcagtatcc ctgttcccgg atggggaaaa ggagtccttt
    14221 gccatagctc aaacggtgaa acctttcttg gataaaatta tgagaagtga ttctttagat
    14281 aaaatcagga aagaattatc ttgatataac tgaatcaggg taactccttt agaggaatat
    14341 aatttttttc tcaccgtcgc ggtgataatt tttttgaata agaatgctct cgaaccctag
    14401 cgaacgagag cattttttta tgaacaatat taaacaatcc cagcagccag cagaaagagt
    14461 tatccgtgag gctgagtgcc ggcagctaac cggtatttgt cgaaccactc gatacatgat
    14521 ggaaaaagag gggagctttc ccgcccgccg caagctggga ggtcgtgctg ttggttggct
    14581 tctgtctgag gtcacagcat ggcagcagag ccgcagcaaa gcagcgtgag gggtgggaaa
    14641 tgtcacataa aacaaaagcg gccatgcagg gccgccaatg tcactaccaa aaacttaagc
    14701 aaagtcagga taccagggtt aatgctagtg gtcaaagcca gagcgctgct gggattaaag
    14761 gaactgcggc attggcgcac accctatttc cccaaatctg gggagaactg gatcatcatg
    14821 cggggtgtgc tgcaatgcag cataccccgg agaaatcccg tagtttcgac aaatacccag
    14881 atattgggtg ttatggcctg cataaaatca cacctgcggt tcttattcct gtccccggct
    14941 cttctttagc tcatttgcaa tggcggatat ttaatcacgt ggtaaccagc ctttcgcatt
    15001 ccttttgggg attggaaggt tcttttcctt caaaatacgt aaaatggcag gttctgaatg
    15061 tggtccccaa ccatcccaat taccttccca acagtcgtta cagcagaaag ccctaccgta
    15121 taattgcagt acacgtcctt ggtaaaggtc tgggccaaat cgtgtttctg atccacagac
    15181 ttcacatgtg caataaaaat gccctttctc atctatgcgc atcatcttct tcctcaaatc
    15241 aaccttttgg ttctattcct tgccgacgca attccgcgcg agctaattct tttatccaat
    15301 taccaagact tacgccttcg cgctccgctg catcgttcag ttgctggcgt agatcagggt
    15361 tgattctgat ctggaaagta ggtgaccagc catcaccttt aggggttttg tctcgtttaa
    15421 ttgttgacac gtacgtacat aacctcatag catgtatttt gttatgtacg tacgttatca
    15481 cgtctggcat agaaaagaca acgccccaaa ctgttggagc agtattgggg cgtctaacca
    15541 aaccgttagt tgaggtaaca gttatggctg aattacagca tacccaaact cgcccggaat
    15601 ttacatggag atttatctct gcttctgagc gctaccctac cgccaaacca ttggtgatct
    15661 acgtcaacgc atccagcgaa caggaggccc gcgacactat gccgggtgta aacctcattt
    15721 tcgcagcacg tctcccattc catgcctttc aggcaatgga ggtacgccat gcgtgaatta
    15781 accaataaaa gtgcgtcaat agcctgtgaa ctggctgcgt tgttgatggt tgtcgaagag
    15841 tgcgacgttg atcaggttga gcgtgaaaac cttatcagtc tggccagacg agtatcggat
    15901 caactggcgg caagcatggt cgagcagaat tcaacggggg ggctcaatgg ataactttta
    15961 cacctaccgc agcaaaaaag atttgctgtt actggcgcaa gaggttgccg cgcttctgtc
    16021 atgtgctgct taccttgcga ctaccagagg cgaggaagag cgtatccatg taatgagttt
    16081 gacgcacctg gctcaacggc tttctgacga gctggcaaac tcactggata tttctacttt
    16141 ttcagaccct gaatcgcggg aggcaaaatc atgatcagca atgtgaagtt ttacgagctg
    16201 gctaagcgcg ttgatctgct ggttgaaaag gttttgcatc ttgaggcaca ggttaagtca
    16261 ctcactgata gtcagggggg ggaaatccct ccgggtatga cgccaatagc aacactggcc
    16321 gctgaatacg gtatctcaac caaaaaggct gaggaactgg cgaaaaacac aggggtgatg
    16381 ctggttaagc tgaaatctgg cgggttcgtt gcacctgatg aaaagttcag ggaagcggcg
    16441 cgtctggtgc tgcgcagcgc taagcgcaaa tatggctctg cgtactggtt ccatcctctg
    16501 atcggcaaat tccagatgag cgggggcatt ccaaaatgac ggtacaactg acagctgtag
    16561 aaaccgtttc tgatgccctg ttcacctgtt cgtatctgtg ggcgcacggc aagcaataca
    16621 gtcgcagcga tttggataaa gcgctccacc agcataagga ccccactacc cagcttggaa
    16681 aagatgtgct tacgctggtg gagcgtggcg acattacagg aatgagcttt ggttttcgcg
    16741 cattatgcga ggagtggagt atcgcgcaaa aaccgtatct gcgtaccgta accgccgctg
    16801 aactccgtga aatcacaata acgtcgatgc ctgcttatcc agaatctggc gtggagattg
    16861 cccgccgttc gttgtttgca cagcaccctg aattacgtcc gacaggaaat aatcgtcatc
    16921 gttggtctga gttggcgggg ttgtgatatg tggtggcctt ttagtcgtaa aaaaagcgag
    16981 cagcgtaacc tgtccattga tgattttctg gcgctgtccg gcgtaccgaa taccggatcc
    17041 ggagaatatg tttctgccgg gacggctgaa tcattgcctg cagtgatgaa cgcggtttct
    17101 gtcatcgctg aggcggtggc cacgatgccg tgttatctgt atctggtacg taatgacaag
    17161 ggcagggagg cgcgggaatg gctggacagt cacccggtag atattctgct gaatgagcag
    17221 cctaattcgt gccagacacc ttaccagttt aaacgcacaa tgatgcgtca ctgcctgctg
    17281 aacggtaacg cctatgcggt tattgagtgg gggcgggacg ggcagcccaa atcacttcat
    17341 ccttatgcac cgggttgtgt tgtaccggaa cgcacaggcg cacacaaata ccgatatacc
    17401 atcaccgaac cctgtacagg aacggtgcgc acgtatttac aggaagaagt tctgcatctc
    17461 cgctatgcct cggatgatgg ctttctgggg cgctcccccg tcacgatttg ccgtgaggca
    17521 cttgggcttg gccttgctca acagcgccac ggagccagca ttatgaaaga tggcatgatg
    17581 gcggcaggga ttatcacgtc aggcgaatgg ctggacggcg tgaaaggtaa acaggcatta
    17641 gatgctctgg aacgctacaa gggggcgaaa aatgccggaa aaacgccaat ccttgaaggg
    17701 ggcatggatt acaggcaact gggaatgagt aaccaggatg cggaatggct ggcctcccgt
    17761 cgcttctcca ttgaagacat cgcccgcatg ttcaacgtat cgcctatttt tctgcaggaa
    17821 tacagcaaca gcacctacag caatttcagc gaggcaagcc gcgcgtttct gaccatgaca
    17881 atgcgcccat ggctggcgaa cttcgaacag caaatcaagg ccgctttgct ggtggcttct
    17941 cccgtacctg gtacccgtta tctggttgag tttgattcag ccgatttatt acgcgccaca
    18001 cccaccgaac gttatgccac gtatgagaaa gggattaaga acgggatcat gaatccgaac
    18061 gaagcccgtg agcgtgaggg tatgccgccg cgtgaaggtg gtgacgagtt cagccaggca
    18121 tggaagcaga ctgtggaaat taaaggtgaa aaagatgagt gaatccagaa ttacgcttaa
    18181 tgaagtaatg gcccatcttc gccttgatga tgatttttat ggtgaggttg aacttctgca
    18241 aatatatacc gatgcggcgc tggaagcctg ccagaagcat atcgggaaac gttttgaaga
    18301 cggcctggaa tttaccccgg caatacgtgt tggttgcctg atgtacatcg cttttctgta
    18361 cgagaaccgg gaagcggttt caccagttga gcactcggaa ctgcctatgg ctatttctgc
    18421 gctctggtcg gtttatcgtg atgtgggggt gtactgatgc cgtggcaacc attaaggcga
    18481 tgcactgagc cgggctgtaa taagcgcgtg aagtccggca agtgtgaaga gcacaggcgg
    18541 gctgcatggc gtgcagagga tgccagacgg ggacaccgcc gcgcacgtgg gtattcccga
    18601 cagtgggaca aataccgcgc cctgtacctg agcaaaaacc cgttatgcgt gcgttgtctg
    18661 gctaagggta tttatacacc agctcttgtg gtggatcaca tcattcccat caatggcggc
    18721 ggtgatgttc tcttctggcc tgagtggaat caccaggcat tgtgccagac gtgccacaac
    18781 cgtaagacga cacgggaaga tccagccacg aaagcaaacc gtaaagcggg catgtatcgc
    18841 gagcaggaag aacgggcggc acaccgtaac gactggatgt atggcgatga tgactgaaca
    18901 ggagcaaacc aggctgatac gtggactgat aaggcagcgt gacacatgga agacacagga
    18961 gacagagcac aaagccaaca ggacagggcg cacaaaacgc accacagcga agcgattaac
    19021 cgaccgtgac cgcgaggtca tggaatgttt tcgcaatcgc tggtgaggcc gtcagagggg
    19081 gtgggggtgg ttttcaggac gaaaccgtcc ctgccggaca ccgaccgccc cctcaaattt
    19141 ttgtgcacgg taattttttt gaaaataatt gggcgaaaaa agaacatggc aagaccacca
    19201 aaagcccccg cttacctgga tgaaatcgcg gtcaggcagt ggaaggaaaa atcgcgccag
    19261 ctttccgggc gggaagacct tacccccgcc gactggagca atctggaact gtattgtgtt
    19321 aactactcca tataccgcaa agccgtcgaa gaccttgcga cgcgcgggtt cagcattgtt
    19381 aacagtcagg gcagcgagag cagaaacccc gcactgagcg caaaggctga cgcagaaaga
    19441 ataatgatca aaatggcttc tttgctgggt tttgacccgg taagccgtcg cagaaatcca
    19501 ccggaaacag aggaagagga cgaacttgac cgcctggcat gagtacgcag aaggcgtaaa
    19561 aaacggcaaa attacggcct gtaaacgact gaaacaggcc gttaaacggt atttttctga
    19621 ccttgaaaac tccctttaca cgttcgatcc ggaggtcgtg gagcggttta ttgccttttc
    19681 ccgggtgtgt ccgcacgtaa aaggcgcaat gcgcggtagt cccattgaac tggagccgtg
    19741 gcagcagttc gcctttgcct gcatcctggg ctttaaggtt aaggccaccg gacggcgcaa
    19801 atacaccagc gcattcattg aagtaccgcg aaaaaatgcc aaatccacgg tcgccgctat
    19861 cctggctaac tggtttctgg ttatggaaaa cgggcagcag gatatttaca ccgccgccgt
    19921 gagtcgtgat caggcgcgga tcgtgtttga tgatgcgcgt cagatgtgcc ttttatcccg
    19981 accgttacga aagcgggtaa atattcaggc gcacaaggtg atacacccga aaaccaacag
    20041 cctgttaaag ccactggcag caaaagcggc aaccattgag gggacaaacc cgagtcttgc
    20101 cattgtggat gaatatcacc tgcacccaga caacggggtt tattccgcac ttgagctggg
    20161 gatgggggcg cgtccggagg ggctgttatt tgccatcacc acatcgggga gcaacgttgt
    20221 ttcagcctgt aaacaacact atgactattg ctgtcagata ctggatggtg aagagatgaa
    20281 cgaatccatg ttcgttctga tttacgagct ggatgatgaa agcgaggttg acgatccggc
    20341 gatgtggata aaggcgaatc caaatatcga tgtttccgtc gatcgtgaaa aactggcctc
    20401 aaccatccag aaagcgcggg gtattccgtc gcagtgggtg gaaatgctca ccaagcgatt
    20461 caatatctgg tgtcaggggg ctacgccgtg gatgggtaac ggtgcatggg cggagtgcgc
    20521 cggaacgttc gcggaggcgg atttatacgg gcaggagtgc tacgcggggc tggacttatc
    20581 atcaaccagc gatatttcca gcgtgtgcta tgcctttccg gtcggtaaaa agattatgct
    20641 ggtttcccgt cactatctgc cggaatttca gctacagaac cccgccaata aaaaccgcgc
    20701 catctatcgc cagtgggtaa aggcgggctg gatacgcaca acaccgggtg actgcattga
    20761 ttatgaccgt atccgtgatg acatcatggc ggatgcagag aatttcaata tcaggctggt
    20821 gggcttcgat acatggaacg ccacgcacct gaggacgcag ctacaggggg caggatttga
    20881 ggtggagccg ttcccgcaaa cataccttcg tttcagtccg gcggcgaaat cgttcgaagt
    20941 ttttgttaac cggaaggtga ttgtgcatcg tggtgatccg gtgctggcct ggtcaatgag
    21001 taatgttgtg atgcagagtg acgcgaacgc caatatcaag ccgaacaaga aaaaatcatc
    21061 caacaagata gacccgagcg ttgcggcgct gatggcgttt ggcacattcc aggcagagca
    21121 tgaggaattt gcatttgata tgagcgacag ccacaaagag cgacttgcgg cgtttgatgg
    21181 ggtatgacga gaatgactga aactgatcta ctaaaaataa ttcgccgcat taccggaatc
    21241 agtcagcagc ctgacgaaca ggctacacag ccggacagcg tgatagccga aaattacgcg
    21301 cgtgtggtgg ctgaggtgat gcgccgtgac ggtattgagc ttaacggcgt ggatatgcgc
    21361 aacatacgaa ccagagtcct tgagttgctg gcataccgtc gccgttctca acaacggagg
    21421 gagagcgcga aaaatactta ccagtggaag aagccggaac gactgcggcg gtaacttgct
    21481 gatattcccg ataacgcaaa attgcgttgg ctggcatagt gcaaatttgc acgatgacct
    21541 aacccattga cttttcttca atcctcaatt ttgaggaggc cggaagataa aaaactgtca
    21601 aaacttgtca tgcaaaacgt taccagcacg gctattgatt tattcggccc ctgcaactgc
    21661 tcccagaatg gtggggatct gaaatccgac cctgaaaaag tgcgcaaaag tggtacgcaa
    21721 aaattagcgt ccagccacag ccaccagcta gacagaacgc ccagagcatt gtcagtaact
    21781 ggttgatatt cccgacagcc ccaaaatggg ttggctggtg ggtgagttgc agatctgcaa
    21841 ctcgaccatg aaactacgga aactacccgt agtttgggta gtaagagtaa cacccagatt
    21901 ctggggctta ctcgcgatac ccaaataaag ggtatcggtg gacgaaatat tatttctcat
    21961 atgtgagcac cgaaggcgaa attccgccct cggttatcta tcatgcacag ggagaagcgg
    22021 ggcaaaactc ttgccatggc gtcttccgga ccgctcacca caccgttttt atccccatta
    22081 aaaatgaaaa aacacatcac tgcggagaca taaccactgg caggtatcta ttctatttat
    22141 tattgttcgg tttcgttcgt gcttatgtgc agtcagtggt gcagtcattt taataaaaaa
    22201 ggcgcttccc catgccgagt agcgcctttt taatcaagca tttagctaac ctgaattagt
    22261 tcatgccgta ttttttcaat tttttacgca gcgtaccacg gttgatgccc atcatcagcg
    22321 cagcacgggt ctggttacca cgggtgtatt gcatcaccat gtccaacagg ggctgttcta
    22381 cttcagccag taccagctca tagaggtcat tcacatcctg accattcagt tgagcaaaat
    22441 agttcttcag tgcctgttta accgagtcac gcaggggttt ttgggttacc tgatcctgag
    22501 agttaacggt agaaacggtc agtacgtcag aatttacgcg ttgttcgaac atagttctgt
    22561 cagctcttta tttctgttta cgcaaaattt tcgaagtatg cctccaacgc ctccagctgt
    22621 tcgctggcat cctcaatggc gttgaatgtg cgccgaaact ggtcatttgg agcgtgttcc
    22681 tggagatacc aggaaacgtg tttacgtgca attcggtacc cttttgccgg accataaaag
    22741 tcatgcagtt cccgaacgtg cgcgcaaagc aagcgcttaa cctctgccaa aggcagcggg
    22801 ggcagcaact ccccagtgtc cagataatgc tggatttccc gaaagatcca gggtcttccc
    22861 tgagctgcgc ggcctatcat cagggcatcc gcccctgtat agtcgagcac agctctggct
    22921 ttaagcgggt cagtaatgtc gccattcgcg ataaccggaa tggaaacttt ctgcttaact
    22981 gcccgaatac tgtcgtactc agcttctcca ttgaacaaac aggcgcgtgt acggccatga
    23041 atggtcagag cctgaatgcc acagtcttca gccagttggg caatctcttc gcagttacgg
    23101 tgttccggtg cccagccggt gcgaatcttc agggtaacag gaacgtccac tgcattgacg
    23161 acctcggtaa ggatcgattt aacgacatcc gggtactgca aaagggctga acctgcgagc
    23221 ttgcgattca cttttttagc cgggcaaccc atattgatat caataatctg ggcaccgctt
    23281 tccacgttaa tacgtgctgc atctgccatt tctttcggat cgctaccagc aatttgcacg
    23341 gtgcgaatac cgggttcatc aatgtgcacc atccgtaaac gagatttgtc gctttcccaa
    23401 acctgtgggt tagaagacat catctcggat actgtcaatc cggctcccat ctcgtagcac
    23461 aacgtccgaa aaggtctgtc tgtaatgcca gccatgggcg ctgcgatcag gcgatttctg
    23521 agctgatatt gtccgatgcg catgagttaa gaaatgacca tactgtgact gcaaggcggc
    23581 gtatattacg cattttttgc acgagatgaa aggccaaact ttgaccaatc ctctgagatg
    23641 gatcaaagaa ttgcatttaa aatgagcgtg gtgcgataat tactcataaa aatcatcata
    23701 ttagaaaata gtgactaaaa tttacactca aagaaatttg agtaagttct caatttttct
    23761 ttatgaatga aaattttggc acgcaaattt gcgtaaataa tcggcaaatt tacgtgcctt
    23821 ttgtgagctt gctcgcactt cgccccgcgt caccctacgg cgatgcgaag gttaattctt
    23881 acgaccggta atgcggcacc actcttcttt ttccacgacc gggtccagtg cgaagctgtc
    23941 gacataagct tcacaaacgc tctctgcctg gcttgccaga ataccggaaa ggcccagcaa
    24001 accgcctgaa accggcagga cgctgattaa cggtgccagt tcacgtaatg ggcctgcaag
    24061 gatgttagcg accaccacgt cggctttcat ttcttctggc tgatctttcg gtaagtagag
    24121 ttccagacgg tcagaaacgc cattacgttc ggcgttgtcg cggctggcct gaatcgcctg
    24181 cggatcgata tcaataccaa tggcttttgc tgcacccagt ttcagcgccg cgatcgccag
    24241 aatgccggaa ccacagccaa agtcgatgac tgttttaccg gttaaatcga ggctgtcgag
    24301 ccattgcagg cacagagagg tggttggatg ggtacccgta ccaaacgcca gccctggatc
    24361 taacatcacg ttgacggcgt tttcgtccgg cacatcacgc cagctagggc agatccacag
    24421 tcgttcacca aagcgcatcg ggtggaaatt atccatccat tcgcgctccc agtctttatc
    24481 ttctagttgt tcgattttat gcgcgaagcc tgcgccgagc agcggatggt tttccagaat
    24541 cgccaccacg tcgttcatat cggtttcagc gtcgaacaga ccaatcacat cggtgtcgcc
    24601 ccacaggcgc gtttcgcccg gcagcggctc aaatactggc gtatcgtggg tatcctgaaa
    24661 agtgatagaa acggcacccg cttccatcag cgcatcgcta agatcttccg cgttcgcgcc
    24721 ggtggtgttc agtttcagtt ggatccaagg catggcaaaa ctctttattt atcagtagtc
    24781 aaaacggtag cttgcgggac ggatgtaccg aaacggtttc cgaccaggaa agccaccaaa
    24841 cttagtagta acgagggcac gatagggtgg aagcccaggt actgaatatt cagcgtcgcg
    24901 agtacggcat acagcacgcc gccaacgatc atcgcactta gcgcgccttt ggcgttggcg
    24961 cgttcccagt aaagacccag caccagcggc cacaggaaaa cggcttccag cccaccgaag
    25021 gccagcaaat tcagccagat gatcatttct ggcggcttcc aggcggcaag cagcagcaac
    25081 gcgccgagaa ctaacgtaat taccgccgac atccgcttca gacgcgtctc gttttgcatt
    25141 tgatccggac ggatattcag atagagatct ttaatgatcg tagcggaact ttgtagcaat
    25201 tgggcgttaa ttgtcgacat gatcgcagcc ataggtgcag ccagaaagat cccggcagca
    25261 aacggtggca gcacttttac cattaacgtc gggatcacca gatccggtac ggtgagatcg
    25321 gggatcactg cacgacctaa cgctccggcc aggtgcatac cgaacatcag gattgcgact
    25381 acaatcgtac cgatgatgat cccccggtgt actgctttgc tgtctttata agagatacag
    25441 cgcaccgcag tatgcggcag gccaatcacg ccaaagcaca ccagtaccca gaacgacgtc
    25501 ataaaggcag gcgacagaat atcgtcagcg ccttgtggcg taaccagttg cggatcgatg
    25561 gtttgcaagg tctgtactgc gttgcttaag ccgccagcgg catgtaccac gccaataagc
    25621 agcacaacgg tgccaatcag catcacaagc ccttgcatgg tgtcgttcag cacgctggcg
    25681 cgaaagccgc caaaggcggt atacaacgca atgctgatac cgaaaatcag cagcccggtt
    25741 tcataaggaa tacccgccgc ggtttccagc aggcgcgcac cgccgataaa ctgcacggtc
    25801 attgccccaa cgaacgcaac cagcaaactc aaactcgcca gccacaccag aagacgactc
    25861 tggtagcggg caaacagcat atcgttcagc gtcactgcat tgtagcggcg cgcaagaatc
    25921 gcgaacttct tgccgagaat accgagcgaa agccagactg caggaagctg aatcatcgcc
    25981 agcaataccc aacccaggcc gtatttataa gcagctccag gcccgccgat aaacgaactg
    26041 gcactgatat aggtcgcggt gagcgtcatc gccagcacaa taccgcccat agagcggctg
    26101 ccgaggaaat actcattaag gaaggtgccg gtgctccgtt tacgcatcgc ataaaccgag
    26161 ataccgaaca ccaccaccag ataggcgacc agcggtagaa ttacttcaag ctgcatcgtc
    26221 atcctccagt gggatatcgc gatagataaa tttcaccatc gcccagcaca gtccaataaa
    26281 cagtagcggc gtcaggatgc aggctatctc aaaccagcgc ggaaagccgg taaaaccggg
    26341 ggcaacgcca ggtaagtaag cggctactaa ccaaactgcc agatacaaaa gggtcagccc
    26401 cagcgcccag cgcgcctctt tatgggcctg aacaaaacga gtgtccattt tttgtccctg
    26461 atgggtgaag aaagcgagga ttgtacctta tgggggttgt cgatccccag taataaaaaa
    26521 ggccggaaaa tccggccttt tgacgctttt gcagtcttat ttttcctgaa gaccgagttt
    26581 tttctccaga tagtggatgt tagtgccacc atgctggaag ttctcgtcat tcatgatgcg
    26641 gatctgcaga tcaacattgg ttttgatacc gtcgatgatc agctcctgca gcgcattctt
    26701 catgcgggca atcgccacgt cacggttttc accgtagcaa atcagcttac cgatcattga
    26761 gtcatagtac ggcggtacgg tgtagcccgc gtagatatga gactcccaac gtacgccaaa
    26821 accgccaggt gcgtggaaac gggtgatttt gcctgggctt ggcaggaagg tgttcggatc
    26881 ttcggcgttg atacgacatt ccaccgcatg gccgcgaacg tgaacttctt cttgcttgat
    26941 cgacagcggt tgaccggcag cgatacgcag ctgttctttg atcaggtcaa cgccggtgat
    27001 catttcggta accgggtgtt ctacctgaat acgggtgttc atttcgatga aatagaactc
    27061 gccgttttcg aacaggaact cgaaagtacc tgcaccgcga tagccgatat caacacacgc
    27121 tttagcgcaa cgttcgccga tgtagcgacg cagttccggg gtaatgcccg gtgctggcgc
    27181 ttcttcgacc actttctggt ggcggcgctg catggagcag tcacgttccg ccagatagat
    27241 agcgttgccc tgaccgtcag ccagtacctg aatctcgacg tggcgaggat tttccaggta
    27301 tttctccatg taaaccatat cgttgctgaa agcagctttc gcttccgcac gggtcatgga
    27361 gatggattgt gccagttcag cgtcgccgcg cactacgcgc ataccgcgac cgccgccgcc
    27421 gccggaggct ttgataatca ccggataacc aatgcgttta gcaatggcgc ggtttttatc
    27481 catatcgtcg cccagcgggc cgtcagaacc aggtacgcaa ggaacgcccg cttttttcat
    27541 cgccgcgatt gcggatactt tgtcgcccat caggcgaatg gtttctgctt tcgggccgat
    27601 gaagataaag ccggagcgtt caacctgctc ggcaaagttg gcgttctcgg agaggaagcc
    27661 gtaacccgga tggattgcta ctgcgccggt gatttcagcg gcgctgatga ttgccgggat
    27721 gttcagataa ctttttactg acggagcagg gccaatacag accgtttcat ctgccagtaa
    27781 tacgtgtttt agatcgcgat ccgcgctgga gtgcacagcg acagtcttga tgcccagttc
    27841 tttacaggca cgaagaatac gcaatgcaat ctcgccgcgg ttggcaataa caattttatc
    27901 cagcatgttc gcctcgttac tcgatgacga ccagcggctc gtcaaattct accggttgtc
    27961 cactttcgac cagaattgct ttcacggtac cggatttgtc cgcttcgatc tggttcatca
    28021 ttttcatggc ttcaacgatg cacagggtat cgcccacgtt gactttctga cccacttcga
    28081 tgaacgcttt tgcgtccggg cttggggtgc ggtagaaagt accaaccatc ggggaacgta
    28141 cgatgtgacc actgatttcc gctgctgctg gcgcttccat ggaaggaacg gtcgccggag
    28201 cggctgcgtt agattgagct ggctgctgca tcattggtgc agcgtaagct tgttgcatca
    28261 cagggaaact tgcggcagga gctgcacggc taatgcgtac tgactcttcg ccttcagaaa
    28321 tttccagttc ggagatgcct gattcttcaa ccagctcgat cagtttttta atcttacgaa
    28381 tatccatgag tgggttccgt actctttgtt tagtgtgatt gtgacaggcg tttcaccgcc
    28441 gtctgtaaag cgtatgcccc gacagctgta tgcatagcga taaattccag caggccggga
    28501 cgccggtcta ttttgcccca gagtgtcaat gttagacttg acggacattg tgcagcgatc
    28561 tgctgctatc ctccggcaaa aaacaaaata taccttcgtt gcgccaatgt caccttttcc
    28621 gcagcgcaaa aactgcgtca gggaggacga ggtcgcacat tataacgatt tcgtagcaat
    28681 tggcagctaa atactggtct tatcagggaa gataatcaac agctaacatg taaataacct
    28741 tcaacaccgt gtaatttgca acaagccgca caattcacga aattagcgcc atcatcgacg
    28801 gaacttctta taacgtaagg tgaaaaactg taaagccagc cctgtgaaga tgaggggctg
    28861 caatgagata atcttcacag accacaaata atgtagggat gccaggatcg cgacaagata
    28921 gacgacatta cgcagcggtt gctgaggatt acccaatttt cactgcatcg tctggataga
    28981 cgtgaatgct aaagcacgca aaatcaaccg actgacaatg ctcagcatta aataggtttg
    29041 agtggttaat ttgtagtcaa ataaaatcag attattcacc ccatggtacc agaaataagt
    29101 aaggggctaa gcgtagcgtc gcccaggcaa agcaccataa acctaacagg cgacggaggc
    29161 gtatcaataa tagctgttta gtggagtatc aatgcgcatc aatttgtggt cagtcgaaat
    29221 catgacttta cagtttggat gatgttgtat aactttctga ccaaggtgtt cgacgaaagc
    29281 tgtccggcta accgcttttc ccgttaaccg attgaataat gatttcaaaa tgagttccct
    29341 ctcttattat tccctgctaa atggttagtt aaccttcacc agcgtgcgac cctggatctg
    29401 gttattaatg atggcctcgg caaagttcgg tgcctctgac agagatatct cttttgccgc
    29461 ctgggtatag aatgattccg gtaaatcggc gaccagtcgc tgccaggctt gtgcgcggcg
    29521 ttctggtggc gtcattactg aatccacccc ttgcaaacgg acattacgca gaataaatgg
    29581 catgaccgtg gttggcagag taaaaccacc cgccagacca caggcagcca cgcagccgcc
    29641 gtaattcatt tgcgccagca cttttgccag cactttgtcg ccaacagtgt caattgcccc
    29701 agcccagact tgtttttcca gaggacggga ttcggcaaac tcatcacgag ggagaatacg
    29761 actggcacct aaacttttca gatattcatg ggtactttcg cgaccggaaa cggcaacgac
    29821 ctgataaccc aacttatgca gcagcgccac ggcggtactg ccgacgccac cactggcacc
    29881 cgtcacgaca atctccccgt cctgcgggcg aacaccggca tcttccagcg ccatcacaca
    29941 cagcatggcg gtaaaaccgg cagtaccgat aatcattgct ttacgcgcgt ccagcccttg
    30001 cggcatggca accagccagt cacctttcac tcgcgcctgc tccgccagcc caccccagtg
    30061 gttttcacca acgccccagc cagtgagtaa catctcctga ccggcatgaa aacgcggatc
    30121 ttcgctggtg cgtacagttc cggcaaaatc gatcccagga atcatcggaa aattacggat
    30181 gatttttccc ttaccggtaa tcgccagcgc atctttatag ttcaggctcg accagtgaac
    30241 atcaaccgtg acatcgccct ccggcaggcg actttcgtca agtgtctgta ccgatgcgag
    30301 agttttgccg tcctgctgtt ctaaaagtaa cgcctgcata agtggtcctc atgtgcatga
    30361 tggattggaa aataatttct gaagactata ctcgctaatg gaaaagcaaa gccgatgaag
    30421 cgcaataaat tgcgagataa atctgatttg ctagtatgcc cgcttcctca ctatcggagt
    30481 taacacaagg atgagattaa cgacgaaatt ttcggccttt gttacgctgc tcaccgggtt
    30541 aacaattttt gtgactttgc tgggctgttc gctaagtttc tacaacgcaa ttcagtataa
    30601 gtttagtcat cgcgttcagg cggtggcgac ggcgattgat acccaccttg tgtcgaatga
    30661 cttcagcaca ttaaggccac aaattaccga attaatgatg tcggcagata tcgttcgtgt
    30721 agacctgctc catggtgata aacaggttta taccctggcc agaaatggta gttatcgtcc
    30781 ggttggctcc agcgatctgt ttcgcgaact gagtgttccg ttgataaagc atccggggat
    30841 gtcgctgcgt ctggtttatc aggatccgat gggcaactat ttccattctt taatgactac
    30901 cgcgccgctc acgggggcga ttggctttat cattgttatg ctcttcctgg cggtacgctg
    30961 gttacaacgg caacttgccg ggcaagaatt gctggaaacc cgggctactc gtatcttaaa
    31021 cggtgagcgt ggctctaatg tgttgggaac catctatgaa tggccgccca gaaccagcag
    31081 tgcgctagat acgctgcttc gtgaaattca gaacgcacgc gaacaacaca gccgtcttga
    31141 tacgctgatc cgctcttatg ccgcccagga cgtgaaaacc ggcctcaata accgactctt
    31201 tttcgataat cagttagcaa cgttactgga agatcaggag aaagtaggta cccacgggat
    31261 cgtgatgatg attcgtctgc cggatttcaa tatgttgagc gatacttggg ggcacagcca
    31321 ggttgaagaa cagttcttca ctctgacgaa tctgctgtcg acatttatga tgcgctaccc
    31381 tggcgcactg ctggcgcgtt accaccgcag tgattttgct gcgctgttac cgcaccggac
    31441 gttaaaagag gcagagagca tcgccggtca gttaatcaaa gccgtcgata ccttgccgaa
    31501 caataaaatg ctcgatcgcg acgatatgat ccacattggt atctgcgcct ggcgtagtgg
    31561 tcaggatacc gagcaggtaa tggaacatgc agagtctgcc acgcgtaatg cgggattgca
    31621 gggcggcaat agctgggcta tttacgatga ctcgttgcct gaaaaaggac gcggtaatgt
    31681 tcgctggcgt acgcttatcg agcaaatgct cagtcgcggc ggcccgcgcc tttatcaaaa
    31741 accggcggtt actcgcgaag gtcaggttca tcatcgcgaa ctcatgtgcc gcatcttcga
    31801 tggtaatgaa gaggttagct cggcggagta tatgccgatg gtcttgcagt ttggcttatc
    31861 ggaagagtat gaccgtctgc aaatcagccg tcttattcca ctattgcgtt actggccaga
    31921 ggaaaatctg gcgattcagg ttaccgttga gtcgctgatt cgcccgcgtt ttcagcgttg
    31981 gctgcgcgat acgttaatgc aatgtgaaaa atcacaacga aaacgcataa ttattgaact
    32041 tgcagaggcc gatgtaggtc aacatatcag tcgtttacaa cctgttattc gtttagtaaa
    32101 tgctttaggg gtacgggtag ccgtcaacca ggctggtttg acgctggtaa gcaccagttg
    32161 gatcaaagaa cttaatgttg agttactcaa gctccatccg gggctggtca gaaacattga
    32221 gaagcgaacg gagaaccagc tgctggttca aagcctggtg gaagcctgct ccgggaccag
    32281 cacccaggtt tacgccaccg gtgtgcgttc gcgaagcgag tggcagaccc tgattcagcg
    32341 cggtgttaca ggcgggcaag gggatttttt cgcgtcctca cagccacttg atactaacgt
    32401 gaaaaaatat tcacaaagat actcggttta acctgccgtt taatccgttt tcacgtagaa
    32461 taacgcgcgc tgcgtctcat gggagtgtgc ttgtctgctc gccagattgt tgcagcacat
    32521 atgcagatga atgaccttac gcggttgcaa acaggcgagg aatgctgctg atgcattaag
    32581 cctttctgga ctcaggcaga gatttgtaac aaaggaaacg aactgcacta attttcaccg
    32641 tagcagatga tttttgcgcc ttgtcgctgc tgcgtgtggt tggtaaagta agcggatttt
    32701 cttttccgcc ccagctttca ggattatccc ttagtatgtt gaaaaaattt cgtggcatgt
    32761 tttccaatga cttgtccatt gacctgggta ctgcgaatac cctcatttat gtaaaaggac
    32821 aaggcatcgt attgaatgag ccttccgtgg tggccattcg tcaggatcgt gccggttcac
    32881 cgaaaagcgt agctgcagta ggtcatgacg cgaagcagat gctgggccgt acgccgggca
    32941 atattgctgc cattcgccca atgaaagacg gcgttatcgc cgacttcttc gtgactgaaa
    33001 aaatgctcca gcacttcatc aaacaagtgc acagcaacag ctttatgcgt ccaagcccgc
    33061 gcgttctggt ttgtgtaccg gttggcgcga cccaggttga acgccgcgca attcgtgaat
    33121 ccgcgcaggg cgctggtgcc cgtgaagtct tcctgattga agaaccgatg gctgccgcaa
    33181 ttggtgctgg cctgccggtt tctgaagcga ccggttctat ggtggttgat atcggtggtg
    33241 gtaccactga agttgctgtt atctccttga acggcgtggt ttactcctct tccgtgcgca
    33301 ttggtggtga tcgtttcgac gaagctatca tcaactatgt gcgtcgtaat tacggttctc
    33361 tgatcggtga agccaccgca gaacgtatca agcacgaaat cggttcggct tacccgggcg
    33421 atgaagtccg tgaaatcgaa gttcgtggcc gtaacctggc agaaggtgtt ccacgcggtt
    33481 ttaccctgaa ctccaacgaa atcctcgaag cactgcagga accgctgacc ggtattgtga
    33541 gcgcggtaat ggttgcactg gaacagtgcc cgccggaact ggcttccgac atctccgagc
    33601 gcggcatggt gctcaccggt ggtggcgcac tgctgcgtaa ccttgaccgt ttgttaatgg
    33661 aagaaaccgg cattccagtc gttgttgctg aagacccgct gacctgtgtg gcgcgcggtg
    33721 gcggcaaagc gctggaaatg atcgacatgc acggcggcga cctgttcagc gaagagtaat
    33781 cggatgcagg caggggaaat gtctgtttac cctgcctggt ctgatacgag aatacgcata
    33841 acttatgaag ccaattttta gccgtggccc gtcgctacag attcgcctta ttctggcggt
    33901 gctggtggcg ctcggcatta ttattgccga cagccgcctg gggacgttca gtcaaatccg
    33961 tacttatatg gataccgccg tcagtccttt ctactttgtt tccaatgctc ctcgtgaatt
    34021 gctggatggc gtatcgcaga cgctggcctc gcgtgaccaa ctagaacttg aaaaccgggc
    34081 gttacgtcag gaactgttgc tgaaaaacag tgaactgctg atgcttggac aatacaaaca
    34141 ggagaacgcg cgtctgcgcg agctgctggg ttccccgctg cgtcaggatg agcagaaaat
    34201 ggtgactcag gttatctcca cggttaacga tccttatagc gatcaagttg ttatcgataa
    34261 aggtagcgtt aatggcgttt atgaaggcca gccggtcatc agcgacaaag gtgttgttgg
    34321 tcaggtggtg gccgtcgcta aactgaccag tcgcgtgctg ctgatttgtg atgcgaccca
    34381 cgcgctgcca atccaggtgc tgcgcaacga tatccgcgta attgcagccg gtaacggttg
    34441 tacggatgat ttgcagcttg agcatctgcc agcgaatacg gatattcgtg ttggtgatgt
    34501 gctggtgacc tccggtctgg gcggtcgttt cccggaaggc tatccggtcg cggttgtctc
    34561 ttccgtaaaa ctcgataccc agcgcgctta tactgtgatt caggcgcgtc cgactgcagg
    34621 actgcaacgt ttgcgttatc tgctgctgct gtggggcgcg gatcgtaacg gcgctaaccc
    34681 gatgacgccg gaagaggtgc atcgtgttgc taatgaacgt ctgatgcaga tgatgccgca
    34741 ggtattgcct tcgccagacg cgatggggcc aaagttacct gaaccggcaa cggggatcgc
    34801 tcagccgact ccgcagcaac cgacgacagg aaatgcagct actgcgcctg ctgcgcccac
    34861 acagcctgct gctaatcgct ctccacaaag ggctacgccg ccgcaaagtg gtgctcaacc
    34921 gcctgcgcgt gcgccgggag ggcaatagtg gcgagctatc gtagccaggg acgttgggta
    34981 atctggctct ctttcctcat tgcgctgttg ctgcaaatca tgccctggcc ggataacctg
    35041 attgttttcc ggccaaactg ggtgttactc atcttgttgt attggatcct ggccttgcct
    35101 catcgcgtaa atgtgggcac aggttttgtg atgggtgcca tactggatct gatcagcggc
    35161 tcgacgcttg gcgtacgcgt attggcgatg agcatcattg cttacctggt ggcgctgaaa
    35221 taccagcttt tccgcaacct cgcattatgg cagcaggcgc tggtcgtcat gttgctttcg
    35281 ctggtggtgg atattattgt tttctgggca gagtttttag tgattaacgt ctctttcaga
    35341 ccggaagtgt tctggagtag tgtagtcaat ggggtgctct ggccgtggat tttcttgctg
    35401 atgcgtaaag tccgtcagca gtttgcagtg caataaaggt ttctatgact tctctgtatt
    35461 tagcttccgg ttctccgcgt cgtcaggagt tacttgcgca acttggcgtg acctttgaac
    35521 gtattgttac gggcattgag gagcagcgtc agccgcagga gagcgcgcag cagtatgttg
    35581 tgcgtctggc gcgcgagaaa gcacaggcgg gtgtcgcgca aacggcgcag gatctcccgg
    35641 tgctgggtgc ggatactatc gttatcctga atggagaagt gctggagaaa ccgcgcgacg
    35701 cagagcatgc ggcgcagatg ttgcgcaaat tatcgggtca gacccatcag gtgatgacgg
    35761 cagtggcgtt ggccgacagc cagcacattc tcgattgcct ggtggtcacc gatgtgactt
    35821 tcagaacgtt aacagacgaa gacatcgcgg gctatgtcgc cagcggtgaa ccgttagata
    35881 aagcaggtgc atacggtatt caggggctgg gtggctgttt tgtcaggaag ataaatggca
    35941 gctatcacgc cgtagtcggc ttaccgctgg ttgaaacgta tgaattatta agtaatttta
    36001 acgcactgcg tgagaaaagg gataaacatg acggctgaat tgttagtaaa cgtaacgcct
    36061 tcggaaacgc gagtggcgta tattgatggc ggtattctgc aggaaattca tattgaacgt
    36121 gaggcgcgac gcggaatagt aggcaatatc tacaagggtc gtgtaagtcg tgtacttccg
    36181 ggtatgcagg cggcttttgt agatattggg ctggataaag ccgcgtttct tcatgcatcc
    36241 gatatcatgc cgcacaccga atgtgtggcg ggtgaagaac aaaagcaatt cacggtgcgc
    36301 gacatctcgg aactggttcg tcaggggcaa gatctgatgg tgcaggtggt gaaagatccg
    36361 cttggcacta aaggtgcgcg cctgaccacc gatatcacgc tgccttctcg ctatctggtg
    36421 tttatgccag gggcttctca cgttggggtt tcccaacgta ttgaaagcga atcagaacgt
    36481 gaacgcctga aaaaagtggt cgcagagtat tgcgacgagc agggcgggtt tatcatccgt
    36541 accgcagcgg aaggggttgg cgaggctgaa ctggcctccg atgccgctta tctgaaacgc
    36601 gtctggacca aagttatgga gcgtaaaaaa cgcccgcaga cccgttatca gctgtacggc
    36661 gaactggcgc tggcgcagcg tgttctgcgt gatttcgccg acgccgaact ggaccgcatt
    36721 cgcgttgact cacgcctgac ttacgaagca ttgctggagt ttacctcgga gtacattccc
    36781 gagatgacca gcaagctgga gcattacacc ggacgccagc cgattttcga tctctttgat
    36841 gtcgaaaacg aaatccagcg agcgctggaa cgcaaagtag aactgaaatc cggtggttat
    36901 ctgattatcg accagaccga agcgatgacc accgtggaca tcaataccgg agcgtttgtc
    36961 ggtcatcgca atcttgacga caccattttc aataccaata ttgaagcgac gcaggctatc
    37021 gctcgccagt tacggttgcg caatctgggc gggattatca ttattgattt catcgatatg
    37081 aataatgaag atcaccgccg ccgagtgctg cactcgctgg agcaggcgtt gagcaaagac
    37141 cgggtgaaaa ccagcgttaa tggtttttcg gcgctggggc tggtggagat gacgcgtaaa
    37201 cgcacccgcg aaagcattga gcacgtactg tgtaacgaat gcccaacctg ccacggtcgc
    37261 ggaacggtga aaaccgtgga aacggtatgc tatgaaatca tgcgcgagat tgttcgtgtc
    37321 caccatgctt acgactccga ccgtttcctg gtctatgctt ctccggcagt agctgaagcc
    37381 ttgaaaggcg aagagtcaca ctcgctggcg gaagtggaaa ttttcgttgg caaacaggtt
    37441 aaagtacaaa ttgaaccgct ctataaccag gagcagtttg acgtcgtaat gatgtaaaca
    37501 gatgctgggc cgccatccgg caaagggttt ttgagtcaca tttttagcag acaaggagtg
    37561 acgggtgagg cgattgccgg ggattttact gcttactgga gccgcgctcg ttgtgatcgc
    37621 tgccctgctg gttagcggcc tgcgtattgc tttaccgcat cttgacgcct ggcgtccgga
    37681 aatcctcaac aaaatagaat ccgcgactgg catgccggta gaagccagtc agctctcagc
    37741 cagctggcag aattttggcc cgacgcttga agcacacgac atccgtgcag aactaaaaga
    37801 tggcggcgaa ttttcggtta aacgcgttac tctggcgctg gatgtctggc agagcttgtt
    37861 acatatgcgc tggcagtttc gcgacctcac tttctggcag ctgcgctttc gcaccaacac
    37921 tcctatcacc agcggtggtg gtaatgatag cctggaagcc agtcacatca gcgatctgtt
    37981 tcttcgtcaa tttgaccatt tcgatctccg cgacagtgaa gtcagttttc tgacgccatc
    38041 cggtcagcgc gccgagctgg cgatcccaca actcacctgg ctgaacgatc cacgtcgaca
    38101 ccgtgcggaa ggcctggtaa gcctctccag ccttaccgga cagcacggcg tgatgcaggt
    38161 gcgtatggat ttgcgcgatg atgaggggtt gttaagcaat ggtcgcgtct ggctacaggc
    38221 ggatgacatc gacctgaagc cgtggctcgg taaatggatg caggacaata ttgctctgga
    38281 aacggcacag ttctcccttg aaggctggat gacgatcgac aaaggcgatg taaccggcgg
    38341 tgacgtctgg ctgaaacagg gcggtgccag ctggttgggc gagaagcaaa cgcatacgct
    38401 gtcggtggat aatctgaccg cgcatattac gcgtgaaaat ccgggctggc agttctctat
    38461 tcccgataca cggatcacga tggacggcaa accctggccg agcggagcat tgacgctggc
    38521 ctggataccg gaacaggacg ttggcggcaa agacaataaa cgcagtgacg aactccggat
    38581 tcgcgccagt aatctggagc tggcaggcct ggagggcgta cgcccgctgg ccgcgaaact
    38641 ttcacctgca ctgggtgatg tttggcgctc cacacaaccg agcggcaaga ttaacactct
    38701 ggcgctggat atcccgcttc aggcggcaga caagacccgt tttcaggcat cgtggagcga
    38761 tctggcctgg aagcaatgga aattattacc gggtgcggaa cacttctccg ggacgctttc
    38821 cggcagcgtt gaaaatggtt tgcttaccgc gtcgatgaag caggcaaaga tgccttacga
    38881 aacggtattc cgtgcgccac tggaaatcgc cgacggccag gcaactataa gctggctgaa
    38941 caatgacaaa ggtttccagc tggatgggcg taatattgac gttaaagcca aagccgtcca
    39001 tgcgcgcggc ggttttcgtt acctgcaacc tgctaacgat gaaccctggc tgggtattct
    39061 ggctggcatc agtaccgatg atggttcaca agcctggcgc tatttcccgg aaaacttgat
    39121 gggtaaagac ctggttgatt atttaagtgg tgcgattcag ggcggtgaag cggataacgc
    39181 gacgctggtt tatggtggca atccgcaact cttcccctat aaacacaacg aaggtcagtt
    39241 tgaagtgctg gtgccgctgc gcaacgcgaa gtttgccttc cagccggact ggcctgcatt
    39301 aactaacctt gatattgaac tggactttat taacgacggt ttatggatga aaaccgatgg
    39361 cgttaatctg ggcggcgtgc gcgcgagtaa tctcaccgca gtgatccctg actactcaaa
    39421 agaaaaactg ctgattgacg ctgacattaa aggtccgggt aaagccgtag gcccttactt
    39481 tgatgagaca ccgctgaaag attccctggg tgcgaccctg caagaactcc agctcgacgg
    39541 cgatgtgaat gctcgcttac atcttgatat cccgctgaac ggcgagctgg taaccgcgaa
    39601 aggtgaagtg acgctgcgta ataacagtct gtttatcaaa ccactcgaca gcaccctgaa
    39661 aaatttgagc ggtaaattca gctttatcaa tggcgatctg caaagtgaac cactgacagc
    39721 aagctggttt aatcagccgt tgaacgtgga tttttccacc aaagaagggg caaaagccta
    39781 ccaggttgcg gtgaatctca acggtaactg gcaaccggcg aaaaccggcg ttttgcctga
    39841 agcggtgaac gaagcattaa gtggcagcgt ggcgtgggat ggtaaagtgg gcattgatct
    39901 gccttatcat gctggcgcga cctataacgt agagctgaac ggcgatctga agaatgtgag
    39961 tagtcactta ccttcaccgt tagccaaacc tgcgggtgaa ccactagcgg taaacgttaa
    40021 ggttgatggc aatctcaaca gctttgaatt aaccggacag gctggtgcgg ataaccattt
    40081 caatagccgc tggttgcttg gtcaaaagct gacgctcgat cgtgctattt gggcggcaga
    40141 cagtaaaacg ctcccgccgt tgccggaaca aagtggcgtt gaactcaata tgccgccgat
    40201 gaatggtgcc gagtggctgg ccctgttcca gaaaggcgct gcggagagtg tcggtggtgc
    40261 agcgagtttc ccacaacaca taacgttacg tacgcctatg ttgtcactgg gaaatcagca
    40321 atggaataac ctgagtattg tttcgcaacc gacggcaaat ggcaccctgg ttgaggcgca
    40381 agggcgtgaa atcaacgcca cgctagcgat gcgtaataac gcgccgtggc tggcgaatat
    40441 caaatatctt tattacaacc cgagcgtggc gaaaactcgt ggtgattcaa cgccgtcatc
    40501 acctttcccg acaacggagc gcattaactt ccgtggctgg ccggacgccc aaatacgatg
    40561 cacagagtgc tggttctggg ggcaaaaatt cggtcgcatt gacagtgata tcaccatttc
    40621 tggcgatacg ttaacgctga ccaatggact gattgatact ggtttctcgc ggcttactgc
    40681 cgatggtgaa tgggttaata atccggggaa tgaacgtacc tcgctgaaag gaaaactgcg
    40741 cgggcagaaa attgatgccg ccgcagaatt ttttggtgtc acgacgccca tacgccagtc
    40801 gtcatttaat gtggattacg atttacactg gcgcaaagca ccctggcagc cagatgaagc
    40861 gacgttgaat ggcatcattc atactcaact gggtaaaggc gaaattaccg aaatcaatac
    40921 cggacatgcc gggcaattgc tgcgcttatt gagcgtagat gccctgatgc gtaagctgcg
    40981 ttttgatttc agagacactt ttggcgaagg gttttatttt gactccattc gcagcaccgc
    41041 gtggattaaa gacggcgtta tgcacaccga cgacacgctg gtggatggcc tggaggcgga
    41101 tatcgccatg aaagggtcgg taaatctggt acgtcgcgac ctgaatatgg aagcggttgt
    41161 cgcaccagag atttctgcga cggtgggcgt ggctgcggct tttgcggtta accccattgt
    41221 tggcgcggca gtgtttgccg ccagtaaagt gctggggccg ctgtggagca aagtctccat
    41281 tttgcgctat cacatttcgg gtccgctgga cgatccgcaa atcaacgaag tgttgcgcca
    41341 accgcgtaaa gaaaaagcgc aatgatttga cgagggcgcg taattgcccc aatctcatat
    41401 gataatcgtt gccaaaggcc aacgagccag aacataaccg taggtcggat agggcgttca
    41461 cgccgcatcc ggcagccgta aaaaatcctc tactgcagta actaacgagt agcaaaaacg
    41521 atgagtctta acctggtaag tgaacaattg ctagcggcga acggcctgaa acatcaggac
    41581 ttgttcgcga tcctcggtca actggccgaa cgtcgccttg attatggcga tctctatttt
    41641 cagtcgagct atcacgaatc ctgggtttta gaagaccgca ttattaaaga tggttcttac
    41701 aacatcgatc agggcgttgg tgtgcgtgca atcagtggtg aaaaaaccgg atttgcttac
    41761 gctgaccaaa tcagcctgct ggcgctggaa cagagtgcgc aagcggcgcg caccatcgtc
    41821 cgtgatagtg gtgatggcaa agtacagacg ctgggcgcgg tagagcatag cccgttgtat
    41881 acctcggtag atccgctgca aagcatgagc cgtgaagaga agctggatat cctgcgtcgc
    41941 gtcgataagg ttgcccgcga agcggacaag cgcgtacaag aagtgactgc cagcctcagc
    42001 ggcgtttatg aattaattct ggttgcggcc accgacggca cgctggcggc ggatgttcgt
    42061 ccgctggtgc gtctttccgt gagcgttctg gtcgaagaag atggcaaacg cgaacgcggt
    42121 gccagtggcg gcggcggtcg ttttggttat gagttcttcc ttgccgatct tgacggcgaa
    42181 gtccgtgcgg atgcatgggc aaaagaagca gtgcgtatgg cgctggtcaa tctttctgcc
    42241 gttgctgcac cagcgggcac catgccggta gtacttggcg caggttggcc gggcgtgctg
    42301 ttgcatgaag cggtaggtca cggtctggaa ggcgacttca accgccgcgg cacttcagta
    42361 tttagtgggc aggtcgggga gctggtggct tcagaactgt gtaccgtggt tgatgacggc
    42421 acgatggtcg atcgccgtgg ttcggtggcg attgatgacg aaggtacgcc aggccagtac
    42481 aacgtgctga ttgagaacgg cattctgaaa ggctacatgc aggataaact caacgcgcgt
    42541 ttgatgggga tgacgccgac tggcaacggt cgccgtgaat cctacgccca tctgcccatg
    42601 ccgcgtatga ccaacaccta tatgctgccg ggtaaatcga ccccgcagga aattattgaa
    42661 tccgttgagt acggtatcta tgcaccgaac tttggtggcg gtcaggtgga tattacatcc
    42721 ggcaaattcg ttttctccac ttcagaagcg tatctgattg aaaacggtaa agtaacgaag
    42781 ccggtgaaag gcgcgacgtt gattggttcc ggtatcgaaa ccatgcagca gatttcgatg
    42841 gttggcaacg acctgaaact ggataacggc gtgggtgtct gcggtaaaga agggcaaagt
    42901 ttgccggttg gcgtgggcca gccaacgctg aaagttgata acctgactgt tggcggtact
    42961 gcgtaataat ttcattattt tcagaaggat aattaatctt tctacgtgca cgaacggtcc
    43021 cctcgctcct ctggggttag ggtgagggga acccgttggc acaggtttgt agaacgtaac
    43081 agtacaatat gaattacttc tctttcccgc gcccgtgcat ctcctgaaac aatttaccga
    43141 cctcaacaaa ataatccgtc agcgagttga tcacgacctg taccttcagc ggcagcttat
    43201 ctttttcggt atataacgca taaaccgggc gtggatctga ctggtaacgt ggtagcagga
    43261 tctccagctc cccgcgattg atctcgttga tcacccacat cagcggcacg taggcgatcc
    43321 cagcgcctgc agtcagccag cgcaccagcg tcatcggatc attagtcaca aatctcccct
    43381 gcgggatcag gcgagtcgag atcccttccg gtgcgatcag ttcaaattca ttgtcgggcc
    43441 gcacgctgta ttcaagccat gaatggctac ttaaatcggc gggtttttcc ggtatgccgt
    43501 attgtgtgag atagcttttt gcggcgcaca ccaccattgg catcgcgccc agacggcggg
    43561 aaaacaggct ggaatcctgc aacgcgccga cgcggatcac cacatccaga ccgtcggcaa
    43621 tcaggtcggg ggcaggaatt ccggtaacca gattgacgct caaacctggg tattctttca
    43681 gcatttttgc tgtcagcccg gcgagaacat tttgtgccat agttgaagaa cagccaatgc
    43741 gcagcgtccc gatgggggtg ttattgaagg catacagttg ctcatgaaca tcctgcactt
    43801 catgaagcat acgacggcag ccctggtagt aaattctacc ggcttcggtc aggccaatgc
    43861 tgcgtgtgct acggtttaac agctttacct gcaactcatc ttccagtttt gacaccgtct
    43921 gactgatgga cgaaacgctc atctgtagct gtctggcggc ggcggtaaaa gagccaaatt
    43981 caactacttt ggcaaacacc gacatgcgtt ttagtcgttc cattattcac tctgacttaa
    44041 aagtgattta gatcacataa tatagataac agcataacag ttacgctaat atattaaata
    44101 tcaatctaca gcaatgttgc tctcgcccgg ctttccatgc ccttctctgc ggcgacagat
    44161 gctgaaaata ataacgcctg ctctctcttt acaaccaagg tcaacatgag tctgtttccc
    44221 gttatcgtgg tgtttgggct gtccttccca ccgatatttt ttgaattgct tttatcactg
    44281 gcgattttct ggctggtgcg ccgggtactt gtgccaacag gtatctacga ctttgtctgg
    44341 catccggcgt tgttcaacac cgcgctctat tgctgcttgt tttatttgat atcgcgactg
    44401 ttcgtttgag gttgaagtga aaacactaat aagaaaattc tcccgtacgg ccatcacggt
    44461 cgtattagtc attctggcct tcatcgcaat ttttaatgcc tgggtctatt acaccgaatc
    44521 cccctggacg cgtgacgcgc gctttagcgc tgacgtcgtt gcgatcgcgc cggacgtttc
    44581 tggactcatt acccaggtga atgttcatga taaccagctg gtgaaaaaag gacagatact
    44641 gttcaccatc gaccagccgc gctatcaaaa ggcgcttgag gaagcgcaag ccgatgttgc
    44701 ttattatcag gtactggcac aggagaaacg ccaggaggcc ggacgtcgta accgtctcgg
    44761 tgtgcaggcg atgtctcgcg aagagatcga ccaggctaac aacgtactac aaacggttct
    44821 gcatcagtta gcgaaagcgc aggcgacccg cgatctggca aaactggatc ttgaacgcac
    44881 ggtgatccgc gcgccagcag atggctgggt gaccaacctc aacgtctata ccggtgagtt
    44941 tattactcga ggatcaacgg cggttgcgct ggtgaaacag aactccttct atgtactggc
    45001 ctatatggaa gaaactaagc tggaaggggt gcgtccgggg tatcgtgcag agatcacgcc
    45061 gcttggcagt aacaaagtgc tgaaagggac tgttgatagt gttgccgcag gggtcaccaa
    45121 cgccagcagc acgcgtgacg acaaagggat ggcgactata gactctaacc ttgaatgggt
    45181 gcgtcttgcg caacgtgttc cggttcgtat tcgtctcgac aaccagcaag agaacatctg
    45241 gcctgcgggc accactgcta cagtggtggt cactggcaaa caagatcgcg acgaaagcca
    45301 ggattcgttc ttccgtaaaa tggcccatcg cctgcgtgag tttggttaat cacgatgggt
    45361 attttctcca ttgctaacca acatattcgc tttgcggtaa aactggcgac cgccattgta
    45421 ctggcgctgt ttgttggctt tcacttccag ctggaaacgc cacgctgggc ggtactgaca
    45481 gcggcgattg ttgccgccgg tccggccttt gctgcgggag gtgaaccgta ttctggcgct
    45541 attcgttatc gtggcttttt gcgcatcatc ggcacattta ttggctgtat tgccggactg
    45601 gtgatcatca ttgcgatgat ccgcgcacca ttattgatga ttctggtgtg ctgtatctgg
    45661 gccggttttt gtacctggat atcctcgctg gtacgaatag aaaactcgta cgcgtggggg
    45721 ctggccggtt ataccgcgct gatcattgtg atcaccattc agccggaacc attgcttacg
    45781 ccgcagtttg ccgtcgaacg ttgtagcgag atcgttatcg gtattgtgtg tgcgattatg
    45841 gcggatttgc tcttttctcc gcgatcgatc aaacaagaag tggatcgaga gctggaaagt
    45901 ttgctggtcg cgcaatatca attaatgcaa ctctgtatca agcatggcga tggtgaagtt
    45961 gtcgataaag cctggggcga cctggtgcga cgcaccacgg cgctacaagg catgcgcagc
    46021 aacctgaata tggaatcttc ccgctgggcg cgggccaatc gacgtttaaa agcgatcaat
    46081 acgctatcgc tgacgctgat tacccaatcc tgcgaaactt atcttattca gaatacgcgc
    46141 ccggaattga tcactgatac tttccgcgaa ttttttgaca cgccggtaga aaccgcgcag
    46201 gacgtccaca agcagctcaa acgcctgcgg agagttatcg cctggaccgg ggaacgggaa
    46261 acgcctgtca ccatttatag ctgggtcgcg gcggcaacgc gttatcagct tctcaagcgc
    46321 ggcgttatca gtaacacaaa aatcaacgcc accgaagaag agatcctgca aggcgaaccg
    46381 gaagtcaaag tagagtcagc cgaacgtcat catgcgatgg ttaacttctg gcgaaccaca
    46441 ctttcctgca ttctgggcac gcttttctgg ctgtggacgg gctggacttc cggcagtggt
    46501 gcaatggtga tgattgcggt agtgacgtca ctggcaatgc gtttgccgaa tccacgcatg
    46561 gtggcgatcg actttatcta cgggacgctg gccgcgctgc cgttagggct gctctacttt
    46621 ttggtgatta tccctaatac ccaacagagc atgttgctgc tgtgtattag cctggcagtg
    46681 ctgggattct tcctcggaat agaagtacag aaacggcgac tgggctcgat gggggcgctg
    46741 gccagcacca taaatattat cgtgctggat aacccgatga ctttccattt cagtcagttt
    46801 ctcgacagcg cattagggca aatcgtcggc tgtgtgctcg cgttcaccgt tattttgctg
    46861 gtgcgggata aatcgcgcga caggactgga cgtgtactgc ttaatcagtt tgtttctgcc
    46921 gctgtttccg cgatgactac caatgtggca cgtcgtaaag agaaccacct cccggcactt
    46981 tatcagcagc tgtttttgct gatgaataag ttcccagggg atttgccgaa atttcgcctg
    47041 gcgctgacga tgattatcgc gcaccagcgc ctgcgtgatg cgccgatccc ggttaacgag
    47101 gatttatcgg cgtttcaccg acaaatgcgc cgcacagcag accatgtgat atctgcccgt
    47161 agcgatgata aacgtcgtcg gtactttggt cagttgctgg aagaactgga aatctaccag
    47221 gaaaagctac gcatctggca agcgccaccg caggtgacgg aaccggttca tcggctggcg
    47281 gggatgctcc ataagtatca acatgcgttg accgatagtt aagtcaaaac cgacgccaaa
    47341 agcgtcggtt ttttcatggc tatacttagc gatgaacggc agaactcgcc gcgaaacgtg
    47401 acggtggcaa cagatgaata tttatacctt tgattttgat gagattgaga gtcaggagga
    47461 tttttatcgc gacttcagcc aagcctttgg tctggcgaaa gataaggtac gcgatctcga
    47521 ctcactatgg gatgtgttaa tgaacgatgt cctgccgcta ccacttgaga ttgaatttgt
    47581 tcatctggga gagaaaacgc gtcgccgttt tggcgcgtta atattgctgt ttgatgaggc
    47641 agaggaagag ctggaagggc atttgcgttt taatgttcgt cattagcgca aaaaaaagcc
    47701 cccgaaccgg gggcaatatc gtcggacaag acgatgaggg tttatttgta cagttcagcc
    47761 gtagcgtgcc aggtgtcacc gctacgagct tcagtaatct ggtaggccgt tgcgcctttc
    47821 tcttccgctt ttttgttcag catttcacgt atatccattg gcgaagacgc cacaccactt
    47881 acggatacgg tcccgattgc ttcacggttt tgtgcttgtg cagcatcaat ggagtcggca
    47941 gcgaatgcac cgaaagagag aacagaaagt acgcttaatg cagcaacagt ggttttgatt
    48001 ttcatgattt ttacctcgac ataatctttt tgctgggtct ttgtttcgtg acccttatca
    48061 caaaatcaag tatacactaa ttattgaact aattaatacc acgctaactg tttctgttga
    48121 tactaagtgt taaaaatgtg aatgttaata acaaaaaaga attaaaaata cggttattta
    48181 tcgttaattc tagtgttttc aattagataa tgaattttgc ataatgtgct ttttttacct
    48241 tatgtattca ttgtgtgaat gacatgtcgc agtaaaacgc actattcgtt aaatgtatcg
    48301 caggagaaag cagggggttg agagggataa gcaacatttt ccccgccgcc agaagcgacg
    48361 gggcagagat taaagctcct ggtcgaacag ctctaaaatc gcttcgtaca ggtctttgac
    48421 ggtgaaaccg ttagcagggg tggtaaagat ggtgtcatcg ccagcgatgg tgcccagaat
    48481 accttctgct ttgcccagtg agtccagcag gcgagcaatt aactgcgccg cgccagggct
    48541 ggtatgaatc acgacaactg catcgttgta gtcgatatcc agcaccagat ttttcaatgg
    48601 actggaggtg gttggtacac ccagttcagc tggcaggcag taaaccattt ccattttggc
    48661 attgcgtgta cgtacagcac caaacttggt caacatccgc gagactttag actgattaat
    48721 attgtcaaag ccttgctcct gcaacgcggc gacgatttcg ccctgggagc taaatttctc
    48781 ttctttaagt aatgctttaa atgctttaac tagttcttct tgcttagccg agcttcgcat
    48841 aagtcacccg atatggtggt tgatacaaca ttattgtgca tacagatgaa tttttatgca
    48901 aacagtcagc cctgaagaag gctgaaataa tgttatgaaa gagcgggatt ttatcaaatt
    48961 tcgttattga gaaacatgcc tgcgtcacgg catgcaaatt ctgcttaaaa gtaaattaat
    49021 tgttatcaaa ttgatgttgt tttggctgaa cggtagggta tattgtcacc acctgttgga
    49081 atgttgcgct aatgcataag cgactgttaa ttacgtaagt taggttcctg attacggcaa
    49141 ttaaatgcat aaacgctaaa cttgcgtgac tacacattct tgagatgtgg tcattgtaaa
    49201 cggcaatttt gtggattaag gtcgcggcag cggagcaaca tatcttagtt tatcaatata
    49261 ataaggagtt taggatgaaa gtcgcagtcc tcggcgctgc tggcggtatt ggccaggcgc
    49321 ttgcactact gttaaaaacc caactgcctt caggttcaga actctctctg tatgatatcg
    49381 ctccagtgac tcccggtgtg gccgtcgatc tgagccatat ccctactgct gtgaaaatca
    49441 aaggtttttc tggtgaagat gcgactccgg cgctggaagg cgcagatgtc gttcttatct
    49501 ctgcaggtgt agcgcgtaaa ccgggtatgg atcgttccga cctgtttaac gttaacgccg
    49561 gcatcgtgaa aaacctggta cagcaagttg cgaaaacctg cccgaaagcg tgcattggta
    49621 ttatcactaa cccggttaac accacagttg cgattgctgc tgaagtgctg aaaaaagccg
    49681 gtgtttatga caaaaacaaa ctgttcggcg ttaccacgct ggatatcatt cgttccaaca
    49741 cttttgttgc ggaactgaaa ggcaaacagc caggcgaagt tgaagtgccg gttattggcg
    49801 gtcactctgg tgttaccatt ctgccgctgc tgtcacaggt tcctggcgtt agttttaccg
    49861 agcaggaagt ggctgatctg accaaacgta tccagaacgc gggtactgag gtggttgaag
    49921 cgaaagccgg tggcgggtct gcaaccctgt ctatgggcca ggcagctgca cgttttggtc
    49981 tgtctctggt tcgtgcactg cagggcgaac aaggcgttgt cgaatgtgcc tacgttgaag
    50041 gcgacggtca gtacgcccgt ttcttctctc aaccgctgct gctgggtaaa aacggcgtgg
    50101 aagagcgtaa atctatcggt accctgagcg catttgaaca gaacgcgctg gaaggtatgc
    50161 tggatacgct caagaaagat atcgccctgg gcgaagagtt cgttaataag taattgatta
    50221 gcgaataata aaaaaccgga gcacagactc cggtttttgt tttgagcact cgacttaatt
    50281 ggttgccgga tattcctgaa tggtgacctg cagcgttaac tgcttatcat cacgcatcac
    50341 tacaaccggg atcaccgaac cagggcgaat ttccgccacc tgatccatcg tctccagagc
    50401 agagatggcc ggtttgttat ccaccgaaat aatcagatcg ttgacctgaa tacccgcatt
    50461 cgccgccggg ccgtcaggtg acacttcatt aaccacgatc ccttgcagtt gatctatacc
    50521 accgccctgc gcgtgcagtg gtgcgatctc gcgtccgccg ataccaatgt agccgcggat
    50581 cacgcgacca tcgcggatca gcttatccat aattttggtt gctaactgga aaggaatcgc
    50641 aaagccgata ccttccggcg tttcgccatc gttactctta tcaaacgaca gcgtgttaat
    50701 gcccatcagt tcgcccagcg agttcaccag cgcgccgcca gagttaccgt ggttaatgga
    50761 agcatcggtt tgtaggaagt tttgccgccc ggtcgggttc agaccgattc gacccgtggc
    50821 actaataatc ccctgggtaa tggtctgccc gaggttgtac gggttaccga tcgccagtac
    50881 tacgtcgcca atgtgcggta cgcgacgtgc attgatcgga atggtaggta aaccgccagt
    50941 ggcattaatt ttaagtaccg ccagatcggt tagagagtca gatcccacca gcaatgcttc
    51001 aaatacacga ccatcctgta aggcgacgat gatctgatcg gcgtcgttga tgacgtgttt
    51061 attggtgatg atataaccgc gttgatccat gattacaccg gatcccaggg tgcggatctc
    51121 aagctggttg tgagagttgg tgttcaaacc acggttgtaa acgttaacca ccgctggcgc
    51181 ggcgcggcga accgccagat tatagctggc aggcgtctca tcggtactgt caaattgcgg
    51241 agtggaaagc gggttaaggc tgcgcagcga aggcatggca accagcagaa tagcgccgac
    51301 aattaatcca atcgcaacgg aacgtaagag cttcacaaac atgatggagg cgtcattaaa
    51361 aaagggaacg gcagcagcat accacgagtt aaccggacat cacacgtaag cctgatgccc
    51421 ggtttacgac attaacgcat cagcagatag atgctttcat tgccgcgtac aatttgcagg
    51481 gcgatgatgg ccggttttgc cgccagcact ttacgcattt cagcaatcga gttcacccga
    51541 tcgcggttga cgccaatgat cacatcgtct ttttgcaagc cagcctgagc agctgggctt
    51601 cctttgacaa cttcatcgat cttaatacct ttgccgccat cttttagctg accatcgctc
    51661 aacgttgcac cttccagcgc tggcgtgatc atttcagcgc tggccgacga agaggtgctg
    51721 gtatcgagcg tcacttctac ttccagtggc ttgccgttac gcagcaggcc aagcttcact
    51781 ttcgtgcccg gctcggtggt cgcgatacga gagcgcaact cagcaaagct attcagcggt
    51841 ttgccgttga ggctggtaat aatatcgccc gctttgacgc ccgctttcgc tgagccagaa
    51901 cctggcaaca cttcgctgac aaacgcgcca cgctgcacgt caaggttgaa ggctttggcg
    51961 atatcggcac tcatctcggt gcctttgatg cctaacaaac cgcgtttgat ttcaccaaag
    52021 tcgataagct gctgcgccag tgttcgcgcc atattactgg ggatggcaaa tccaatcccg
    52081 acgctcccgc cgccaggcgc aaggattgca gtgttgatgc caattaactc accgttaagg
    52141 tttaacagtg caccgccgga gttaccgcgg ttaatggaag catctgtctg gataaagttt
    52201 tccagacctt caagattcaa cccgctgcgt cctaatgcgg aaacaatgcc agaggtggcg
    52261 gtttgcccaa ggccaaatgg gttaccgacc gctacggcaa aatcaccgac gcgcaattta
    52321 tcggagtcgg caatagcgat ttgcgttaat ttgctcgggt tttgaatttg taacagggcg
    52381 atatcgctct ggtcatcgct accaatcagt tttgcatcaa actcgcgccc atcattgagc
    52441 tgaatactga ttttctgtgc ctgattaatc acatggttgt tggtcagcac atagccttta
    52501 ctggcgttga tgatgacacc ggaacctaaa ccttcgaagg gttgtgcagg ttgatccggt
    52561 aaatcatcac caaaaaactt tttgaattct tccgggattt tctgtccctg actggccgtt
    52621 ccttccaccc gtacgctcac cactgccgga agcacttttt ccagcattgg agccagactg
    52681 gggagagggg cctgatcggc aacctggcct ggaatcgacg cgacggcctg aaatgacgcc
    52741 gagagagtta acccgacact taacgctaat gcactcaaca gctgggtttg ttttttcatt
    52801 attcctgctc tcgtacctga atgataagaa aagagattca aaacagtttg atgttattga
    52861 attttcagac ggctaacaat gagacaccag attaaatgat agtcggggag ggagagaaga
    52921 ggagggcgca atggctgcgc ccgaaaaata aattagtcgc gcttcgcgcc agtacgcagc
    52981 aggccggatg cgccttcaga atagtcgcga ggcatctgca ccggtgcctg atcgttgctg
    53041 gcttcagact ctgccagacg attacggaac gggtttgctt cagcagacag ttccggcagc
    53101 aggctgctgg agcttttcgc catgtgctga tacagctggc gatagtcgtg cgccatggta
    53161 tccagtaatt ccgcgctgcg ggcaaagtgg ctaaccagct cttcgcgata ctcgtccagt
    53221 tcagctttat tcttttccag ttcgtactgc aacgcctgtt gctggcgtag tttacgatta
    53281 ccaaaacgca tggccacagc accaataatg atgccgacga ctaacccaat tagcgcatat
    53341 tcccaggtca tgaacatctc ccgttgtctt ttgtttccgt agggtgttgg cttcaggctc
    53401 catgcctgcg gctgattatg ccactataac cgttaattcc acagaagtgg aatcccgact
    53461 gcatatcgcg tagtgtagaa cggccttttt ttcgtcaacc gtgaacaacg gcgcaccgat
    53521 tattcaagga ataacaataa gatcatgcaa agcgttaccc caacatcgca atacctgaag
    53581 gcgctcaatg aaggcagcca tcaacccgac gacgttcaaa aagaggccgt cagccgcctg
    53641 gaaattattt atcaggaact catcaatagc acgccaccag cccccaggac gagtgggcta
    53701 atggcgcggg tcggtaagct gtggggtaaa cgcgaagaca caaagcatac gccagtgcgt
    53761 ggcttatata tgtggggcgg tgtaggacgc gggaaaacct ggctgatgga ccttttctat
    53821 caaagcctgc cgggagagcg gaaacagcgc ctgcactttc accgttttat gatgcgggtg
    53881 catgaagagc taactgcctt acaggggcag accgatccgc tggaaattat tgccgatcgc
    53941 tttaaagccg aaactgacgt gctctgtttt gacgaatttt ttgtttctga tattaccgac
    54001 gccatgctac ttggcggtct gatgaaagcc ctgttcgccc gcggcattac cctggtagcg
    54061 acgtcaaata ttccgccgga tgaactttat cgaaatggcc tgcaacgtgc gcgttttctg
    54121 cctgcaatcg atgccattaa acagcattgt gatgtaatga acgtggacgc tggtgttgat
    54181 tatcgactgc gtacactcac tcaggcgcat ctgtggcttt cgccactcaa cgatgaaacc
    54241 cgggcgcaga tggataaact atggttggcg ctggcggggg cgaaacgaga aaattcaccg
    54301 acgttagaaa tcaaccatcg gccattggcg acaatgggcg tcgagaacca gacgctggcg
    54361 gtctctttta ctacgctgtg cgtcgacgcc cgcagtcagc atgactatat tgcgctctca
    54421 cgtctctttc atacggtcat gttgtttgat gtaccagtta tgacgcggtt gatggagagc
    54481 gaagcgcggc gctttattgc gctggtggat gagttttacg agcgccatgt caaattagtg
    54541 gtgagtgcag aagtgccgct gtatgaaatt tatcagggcg atcggctgaa gtttgagttc
    54601 cagcgttgcc tgtcacgtct gcaagagatg caaagcgaag agtatctgaa gcgcgagcat
    54661 ttggcgggtt aaaacctgtc acaaatcaca aaaaggggtc gatctttgac cccgacttct
    54721 ctataatcct gcgaccccac gttacaagaa agtttttttc ccaaaacttt ttgtgtgctg
    54781 gcataggcta ttcgaagggg taggtttgcc ggactttgtc gtgtgaacct caacaattga
    54841 agacgtttgg gtgttcacca acgtgtaact atttattggg taagctttta atgaaaactt
    54901 ttacagctaa accagaaacc gtaaaacgcg actggtatgt tgttgacgcg accggtaaaa
    54961 ctctgggccg tctggctact gaactggctc gtcgcctgcg cggtaagcac aaagcggaat
    55021 acactccgca cgtagatacc ggtgattaca tcatcgttct gaacgctgac aaagttgctg
    55081 taaccggcaa caagcgtact gacaaagtgt actatcacca caccggccac atcggtggta
    55141 tcaaacaagc gacctttgaa gagatgattg ctcgccgtcc tgagcgtgtg attgaaatcg
    55201 cggttaaagg catgttgcca aaaggcccgc tgggtcgtgc tatgttccgt aaactgaaag
    55261 tttacgcggg taacgagcac aaccacgcgg cacagcaacc gcaagttctt gacatctaat
    55321 cgggattata ggcaatggct gaaaatcaat actacggcac tggtcgccgc aaaagttccg
    55381 cagctcgcgt tttcatcaaa ccgggcaacg gtaaaatcgt aatcaaccaa cgttctctgg
    55441 aacagtactt cggtcgtgaa actgctcgca tggtagttcg tcagccgctg gaactggtcg
    55501 acatggttga gaaactggac ctgtacatca ccgttaaagg tggtggtatc tctggtcagg
    55561 ctggtgcgat ccgtcacggt atcacccgcg ctctgatgga atacgacgag tccctgcgtt
    55621 ctgaactgcg taaagctggc ttcgttactc gtgacgctcg tcaggttgaa cgtaagaaag
    55681 tcggtctgcg taaagcacgt cgtcgtccgc agttctccaa acgttaattg gcttctgctc
    55741 cggcagaaaa caatttgcga aaaaacccgc ttcggcgggt ttttttatgg ttaaattctg
    55801 aatcagcgta aaaattggaa agttgctttt tgctaccgtc tgacagacag gcaaaacaaa
    55861 aaccacatcg ccaataaggg actaagtcaa ctatttcaga ctaaagcgca tctctttttc
    55921 cccatttccg gcatcgactc accacaaagg tcgcaaaatc tggtaaacta tcatccaatt
    55981 ttctgcccaa atgtcgggta ttgctcattt tttgtttgat tttcgaacaa agagagtcgt
    56041 tccttactgg gtaacacaac ttctgactgg ccacctggtg gctggtagca gtaaaaattc
    56101 tgactatacc tggaggtttt catggctgtc gctgccaaca aacgttcggt aatgacgctg
    56161 ttttccggtc ctactgacat ctatagccat caggtccgca ttgtgctggc tgagaaaggt
    56221 gtaagtttcg agatcgaaca cgtggaaaag gacaatccgc ctcaggatct gattgacctc
    56281 aacccgaatc agagcgttcc gaccctggtg gatcgtgagc tgaccctgtg ggaatctcgc
    56341 atcattatgg aatatctgga tgagcgtttc ccgcatccgc cactgatgcc tgtttacccg
    56401 gtagctcgcg gtgaaagccg tctgtacatg catcgtatcg aaaaagactg gtacacgctg
    56461 atgaacacca tcatcaacgg ttcagcttct gaagcagatg ccgcacgtaa gcaactgcgc
    56521 gaagaactgc tggcgattgc gccggtcttc ggtcagaagc cgtacttcct gagcgatgag
    56581 ttcagcctgg tcgattgcta tcttgctccg ctgctgtggc gtctgccgca actgggcatc
    56641 gagttcagcg gcccgggtgc gaaagagctg aaaggctata tgacccgcgt ctttgagcgt
    56701 gactctttcc ttgcttcttt aactgaagca gaacgtgaaa tgcgtctggg ccggagttaa
    56761 tctgtatgga tttgtcacag ctaacaccac gtcgtcccta tctgctgcgt gcattctatg
    56821 agtggttgct ggataaccag ctcacgccgc acctggtggt ggatgtgacg ctccctggcg
    56881 tgcaggttcc tatggaatat gcgcgtgacg ggcaaatcgt actcaacatt gcgccgcgtg
    56941 ctgtcggcaa tctggaactg gcgaatgatg aggtgcgctt taacgcgcgc tttggcggca
    57001 ttccgcgtca ggtttctgtg ccgctggctg ccgtgctggc tatctacgcc cgtgaaaatg
    57061 gcgcaggcac aatgtttgag cctgaagctg cctacgatga agataccagc atcatgaatg
    57121 atgaagaggc atcggcagac aacgaaaccg ttatgtcggt tattgatggc gacaagccag
    57181 atcacgatga tgacactcat cctgacgatg aacctccgca gccaccacgc ggtggtcgac
    57241 cggcattacg cgttgtgaag taatacaaaa acaggccctg gtggcctgtt ttgtctttta
    57301 atggcttaat aaaatagcgt gattgtggca atcatattca ccacgaaacc gactgccatg
    57361 gggatcgctg ttcgcttcac caccgcaaaa ggcgaaacgc ctgcaattcc tgacgtaacg
    57421 accaccaccg cagtaatcgg cgaaaccgcg cgcgctagcg tcgtggcaaa atgcatcggc
    57481 ataatcatta caaccgctgg tacatgtagt ccggctgcga tattcggaat aagactggca
    57541 aatgacataa acggcgcatt gccagagccc atcacaatgg cacaaatggc aatgactagc
    57601 gccataataa tcatcacgcc aataccgccc agaccagaat gctccgcacc cctgataacc
    57661 gcatcaacag tgccaatcgt cgttaagcct ttcgcaaata tttcacccgc gaccaccagc
    57721 gttaccacgt tggcaaactg cgtacccatg ccgtcaaaaa acgcctgcac atcgtccatt
    57781 gtttcgcgca agttatgctt gcggaagaac tcaacaaaca tcgtcaccgt caaactcagt
    57841 aacatcacca ccaccagatg cagttctgac tgcatcagcc cgacgtgggc gaggaacagc
    57901 gagccgagca tcaggattaa cggcatcaca ggtaaaatgg cgtaatagag cggcgggaca
    57961 ttatcgagag ctttttgctc tgcttgttcg tgattgatat ttttatcttt tttgtcaaaa
    58021 gcgcgttgca cgaaaaagtg ggagatcgcc accgagataa tgacgcaaga ggcgaccgga
    58081 agctggtagt ggaagaagta agtggcaatt ttcattcccg cgacctgggc tgcaaaaatg
    58141 gagttcgttt ccagaattcc ccactcaatg gacattgtgg ttgcgataac cgccaccgca
    58201 gagagacgac ttactcccag actcaccagt gtcggaaata aggtgaccat cagcaacata
    58261 cccagaccgg aggcgctggt aataaactgc gccatgattt gaccgatgac gtaagttgcc
    58321 gacagaataa tatacggcga gcgaatgagt tttaacgggc ggcttaacaa gctcaccatc
    58381 gcgcgactgg cccctatgcg ctccatgtag cgggcataac cgcccaccgc cataatcgat
    58441 agccccaatc cggcaatgcg gttggacagc atgcgcagga tttcattata aatatcgacg
    58501 atcaagtagc cgctgctttt ggcgggatcg agtacaccac cgaaccctaa ccagacaccg
    58561 cacatcatca ggaagatacc tgcggcagcc agcaccacct gaggtttgta gtttttcagg
    58621 atcaaatagc ccatcgtaat taatacgatg acagatataa ttatgccgaa cattgttgaa
    58681 ctccgtgtca aaagaaaacg gtcaatccca taaacggcag attgaaaaca atgatgttat
    58741 attttttgca agtctattta tggtacggat gtcgtgtttt taattgtagg tgaggtgatt
    58801 tttcattaaa aaatatgcgc ttatgattat tttgtaagaa cacattcata atattcataa
    58861 tgctcgtgaa tagtcttata aataattcaa acgggatgtt tttatctgcg ttacattaat
    58921 ttttcgcaat agttaattat tccgttaatt atggtaatga tgaggcacaa agagaaaaac
    58981 ctgccatttt cccctacttt caatcctgtg ataggatgtc actgatgatg ttaatcacac
    59041 tgaccttaca gaatgggcct tatgaacgca tttgattcgc aaaccgaaga ttcttcacct
    59101 gcaattggtc gcaacttgcg tagccgcccg ctggcgcgta aaaaactctc cgaaatggtg
    59161 gaagaagagc tggaacagat gatccgccgt cgtgaatttg gcgaaggtga acaattaccg
    59221 tctgaacgcg aactgatggc gttctttaac gtcgggcgtc cttctgtgcg cgaagcgctg
    59281 gcagcgttaa aacgcaaagg tctggtgcaa atcaacaacg gcgaacgtgc ccgcgtctca
    59341 cgtccttctg cggacaccat catcggcgag ctttccggca tggcgaaaga tttcctttct
    59401 catcctggtg ggattgccca tttcgaacaa ttacgtctgt tctttgaatc cagcctggtg
    59461 cgctatgcgg ctgaacatgc caccgatgag caaatcgatt tgctggcaaa agcactggaa
    59521 attaacagtc agtcgctgga taacaacgcg gcattcattc gttcagacgt tgatttccac
    59581 cgcgtgctgg cggagatccc cggtaaccca atcttcatgg cgatccacgt tgccctgctc
    59641 gactggctta ttgccgcacg cccaacggtt accgatcagg cactgcacga acataacaac
    59701 gttagttatc aacagcatat tgcgatcgtt gatgcgatcc gccgtcatga tcctgacgaa
    59761 gccgatcgtg cgttgcaatc gcatctcaac agcgtctctg ctacctggca cgctttcggt
    59821 cagaccacca acaaaaagaa ataatgccac tttagtgaag cagatcgcat tataagcttt
    59881 ctgtatgggg tgttgcttaa ttgatctggt ataacaggta taaaggtata tcgtttatca
    59941 gacaagcatc acttcagagg tatttatggc aacgaattta cgtggcgtaa tggctgcact
    60001 cctgactcct tttgatcaac aacaagcact ggataaagcg agtctgcgtc gcctggttca
    60061 gttcaatatt cagcagggca tcgacggttt atacgtgggt ggttcgaccg gcgaggcctt
    60121 tgtacaaagc ctttccgagc gtgaacaggt actggaaatc gtcgccgaag aggcgaaagg
    60181 taagattaaa ctcatcgccc acgtcggttg cgtcagcacc gccgaaagcc aacaacttgc
    60241 ggcatcggct aaacgttatg gcttcgatgc cgtctccgcc gtcacgccgt tctactatcc
    60301 tttcagcttt gaagaacact gcgatcacta tcgggcaatt attgattcgg cggatggttt
    60361 gccgatggtg gtgtacaaca ttccagccct gagtggggta aaactgaccc tggatcagat
    60421 caacacactt gttacattgc ctggcgtagg tgcgctgaaa cagacctctg gcgatctcta
    60481 tcagatggag cagatccgtc gtgaacatcc tgatcttgtg ctctataacg gttacgacga
    60541 aatcttcgcc tctggtctgc tggcgggcgc tgatggtggt atcggtagta cctacaacat
    60601 catgggctgg cgctatcagg ggatcgttaa ggcgctgaaa gaaggcgata tccagaccgc
    60661 gcagaaactg caaactgaat gcaataaagt cattgattta ctgatcaaaa cgggcgtatt
    60721 ccgcggcctg aaaactgtcc tccattatat ggatgtcgtt tctgtgccgc tgtgccgcaa
    60781 accgtttgga ccggtagatg aaaaatatct gccagaactg aaggcgctgg cccagcagtt
    60841 gatgcaagag cgcgggtgag ttgtttcccc tcgctcgccc ctaccgggtg aggggaaata
    60901 aacgcatctg taccctacaa tttttatacc aaagcgtgtg ggcatcgccc accgcgggag
    60961 actcacaatg agtactacaa cccagaatat cccgtggtat cgccatctca accgtgcaca
    61021 atggcgcgca ttttccgctg cctggttggg atatctgctt gacggttttg atttcgtttt
    61081 aatcgccctg gtactcactg aagtacaggg tgaattcggg ctgacgacgg tgcaggcggc
    61141 aagtctgatc tccgcagcct ttatctctcg ctggttcggc ggcctgatgc tcggcgctat
    61201 gggtgaccgc tacgggcgtc gtctggcaat ggtcaccagc atcgttctct tctcggccgg
    61261 gacgctggcc tgcggctttg cgccaggcta catcaccatg tttatcgcgc gtctggtcat
    61321 cggcatgggg atggcgggtg aatacggttc cagcgccacc tatgtcattg aaagctggcc
    61381 aaaacatctg cgtaacaaag ccagtggttt tctgatttca ggcttctctg tgggggccgt
    61441 cgttgccgct caggtctata gcctggtggt tccggtctgg ggctggcgtg cgctgttctt
    61501 tatcggcatt ttgccaatca tctttgctct ctggctgcgt aaaaacatcc cggaagcgga
    61561 agactggaaa gagaaacacg ggggtaaagc accggtacgc acaatggtgg atattctcta
    61621 ccgtggtgaa catcgcattg ccaatatcgt aatgacactg gcggcggcta ctgcgctgtg
    61681 gttctgcttc gccggtaacc tgcaaaatgc cgcgatcgtc gctgttcttg ggctgttatg
    61741 cgccgcaatc tttatcagct ttatggtgca gagtacgggc aaacgctggc caacgggcgt
    61801 aatgctgatg gtggtcgtgt tgtttgcttt cctctactca tggccgattc aggcgctgct
    61861 gccaacgtat ctgaaaaccg atctggctta tgacccgcat actgtcgcca atgtgctgtt
    61921 ctttagcggc tttggggctg cggtggggtg ctgtgtcggc ggcttcctcg gtgactggct
    61981 gggaacccgc aaagcctacg tttgtagcct gttggcctcg cagctgttga ttattccggt
    62041 atttgcgatt ggcggcgcaa acgtctgggt gctcggtctg ttactgttct tccagcaaat
    62101 gcttggacaa gggatcgccg ggatcttacc gaaactgatt ggcggttatt tcgataccga
    62161 ccagcgtgca gcgggcctgg gctttaccta caacgttggc gcattgggcg gcgcactggc
    62221 cccaatcatc ggcgcgttga tcgctcaacg tctggatctg ggtactgcgc tggcatcgct
    62281 atcgttcagt ctgacatttg tggtgatcct gctgattggt ctggatatgc cttcccgcgt
    62341 tcagcgttgg ctgcgcccgg aagcattgcg tactcatgac gctatcgacg gcaaaccatt
    62401 tagcggtgcc gtgccatttg gcagcgccaa aaacgattta gtcaaaacca aaagttaatc
    62461 ctgttgcccg gtctatgtac cgggcctttc gctaagggaa gatgtatgtc gttacttgca
    62521 caactggatc aaaaaatcgc tgctaacggt ggcctgattg tctcctgcca gccggttccg
    62581 gacagcccgc tcgataaacc cgaaatcgtc gccgccatgg cattagcggc agaacaggcg
    62641 ggcgcggttg ccattcgcat tgaaggtgtg gcaaatctgc aagccacgcg tgcggtggtg
    62701 agcgtgccga ttattggaat tgtgaaacgc gatctggagg attctccggt acgcatcacg
    62761 gcctatattg aagatgttga tgcgctggcg caggcgggcg cggacattat cgccattgac
    62821 ggcaccgacc gcccgcgtcc ggtgcctgtt gaaacgctgc tggcacgtat tcaccatcac
    62881 ggtttactgg cgatgaccga ctgctcaacg ccggaagacg gcctggcatg ccaaaagctg
    62941 ggagccgaaa ttattggcac tacgctttct ggctatacca cgcctgaaac gccagaagag
    63001 ccggatctgg cgctggtgaa aacgttgagc gacgccggat gtagggtgat tgccgaaggg
    63061 cgttacaaca cgcctgctca ggcggcagat gcgatgcgcc acggcgcgtg ggcggtgacg
    63121 gtcggttctg ccattacgcg tcttgaacac atttgtcagt ggtacaacac agcgatgaaa
    63181 aaggcggtgc tatgaccacg ctggcgattg atatcggcgg tactaaactt gccgccgcgc
    63241 tgattggcgc tgacgggcag atccgcgatc gtcgtgaact tcctacgcca gccagccaga
    63301 caccagaagc cttgcgtgat gccttatccg cattagtctc tccgttgcaa gctcatgcgc
    63361 agcgggttgc catcgcttca accggaatta ttcgcgacgg cagtttgctg gcgctgaatc
    63421 cgcataatct cggtggattg ctacactttc cgttagtcaa aacgctggaa caacttacca
    63481 atttgccgac cattgccatt aacgacgcgc aggctgcagc atgggcggag tatcaggcgc
    63541 tggaaggcga tgtaaccgag atggtgttta tcaccgtttc cactggcgtc ggcggcggtg
    63601 tagtgagcgg cggcaaactg cttaccggcc ctggcggtct ggcggggcat atcgggcata
    63661 cgcttgccga tccacacggc ccggtctgtg gctgtggacg cacaggttgc gtggaggcga
    63721 ttgcttctgg tcgcggcatt gcggcggcag cgcagggtga actggcgggc gcggatgcga
    63781 gaactatttt cacacgcgcc gggcagggcg acgagcaggc gcagcagctg attcaccgct
    63841 ccgcacgtac gcttgcaagg ctgatcgctg atattaaagc cacaactgat tgccagtgcg
    63901 tggtggtcgg tggcagcgtt gggctggcag aagggtatct ggcgctggtg gaaacgtatc
    63961 tggcgcagga gccagcggca tttcatgttg atttactggc ggcgcactac cgccacgatg
    64021 caggtttact tggggctgcg ctgttggccc agggagaaaa attatgatga tgggtgaagt
    64081 acagtcatta ccgtctgctg ggttacatcc tgcgttacag gacgcgttaa cgctggcatt
    64141 agctgccaga ccgcaagaaa aagcgccggg tcgttacgaa ttacagggcg acaatatctt
    64201 tatgaatgtc atgacgttta acactcaatc gcccgtcgag aaaaaagcgg aattgcacga
    64261 gcaatacatt gatatccaac tgttattaaa cggtgaggaa cggattctgt ttggcatggc
    64321 aggcactgcg cgccagtgtg aagagttcca ccatgaggat gattatcagc tttgcagcac
    64381 cattgataac gagcaagcca tcatcttaaa accgggaatg ttcgccgtgt ttatgccagg
    64441 tgaaccgcat aaaccaggat gcgttgtcgg cgagcctgga gagattaaaa aggttgtggt
    64501 gaaggttaag gctgatttaa tggcttaaaa cttagttcgt tttaaagatt ctgcagacac
    64561 tgtctgcaga attcgaaacc gctgaaatta acggcgtcta tagccttcct ctagcaccgc
    64621 ctgtgttcga acgagttcat ctgcgagtcg tttctcatca ggtagttgca tcttatattc
    64681 gcttgccaga acggtgttag gcaaacctgc cagagcataa tgtgcttctc cggctccttt
    64741 ctctgcacag agaaccagac cgatgggcgg attttcatcc ggtagcgtcc agtgctcttt
    64801 ggcgtagttg agatacatat tcatctgtcc ggcatcgcta tagctgaatt tgcccacttt
    64861 tagatcgacg attagtaggc agcgtaaacg gcggtggaaa aacagcagat cgacacgaaa
    64921 ccagttgtca tcaatgcgta accttcgctg ccgaccaaca aaggcaaaat catccccaag
    64981 ttccagcatg aaatccatca ggtggttgat cagcgcctcc tcgaaatcag attctgaata
    65041 ttcatctttc aattccagaa attcgagcac aaagggatcg cgtatcgcct gttgcggaag
    65101 aatatgcgtc tcggtaggag tgtgttgttg cagcatggct gatttgtcat gtgacagtag
    65161 tgttcgctca taaaattggg tcgcaatttg ccgttctagc tggcgaacag accagccaca
    65221 gcggagcgtc tctttttcat aaaagctgcg ggcgtcagcg tttttaacgg aaagtaaacg
    65281 gacgtaggtg gaccagggca gggggaaaga tttggcagac agcgtctgcc aaatctttgc
    65341 cgatgggaat tctgcagatg gtgtctgcgg aattcccaat ggtgttaatt cgccagacac
    65401 tgtctggcga atttcaacat gttgaaaaaa gaggtaaaaa agcctcattt ggcgtaagtt
    65461 ttttgcagag aacccacgct tataccttag acttaaatcc tttgatagtc gcttgattag
    65521 ctgcgcacca tacgcagccc tggcctcgcc accttgttca aattcgacaa ttcgtcggcc
    65581 aatttcccag tatgtcgcgg tcatcagcgc gttaacgcta cgtaccgttt ccgtccgggc
    65641 gctatcgacc agatgaataa taccgtcgtg gatttgctgg tagcctgttg aggttctctc
    65701 tgagagcgat tccattatct gtccctttat atggcgctgc gttgaataat ggaatcagct
    65761 atacgccggg catctaccgg taacaatcca gatgagcgta ttacattggt tttcttcaga
    65821 ttatgcgagg cgctttccag aaagaaagga gaatttcagc gccgactctt ttctgaggag
    65881 gcggcgctgg gacagagctt aaacttccag ccagttcata ataccgtctg ccgccttacg
    65941 accttcggca atagcggtta ccaccagatc ggaaccacgg acgatatcgc cgccagcaaa
    66001 gattttcggg ttgctggtct ggaaggcgtt gtcgctgcct tccggggcga tgatgcggcc
    66061 ttgtgaatcc agctcgacgc tgtgttttgc cagccattcc atgttgtgtg gacggaaacc
    66121 aaacgccatg atcactgcat ctgccggaac gatatgttcg gaacctgcaa cgatctccgc
    66181 gcggcgacgg cctttggcat ccggttcgcc catttcggta cgtaccattt ttacgccgct
    66241 gactttaccg ttaccgttca cttcaatccc cagtggctgg acgttgaatt tgaactctac
    66301 gccttcttcc cgcgcgtttt tcacttcgcg gcgggaaccc ggcatgttct cttcatcacg
    66361 acgataggca caggtaacgt gcttcgctcc ctggcgcacg gacgtacgca cgcagtccat
    66421 cgcagtgtcg ccaccgccaa ggaccaccac gcgtttgcct tccatgctga cgaacggttc
    66481 gtcgcgggtt tcaccaaagc ccattaactg tttggtgttg gcgataagga acggcagcgc
    66541 tgcgtacacg ccatcggcgt cttcgttttc cagcccgccg cgcattgact gataagtccc
    66601 gacgccaagg aagacggcat cgtaatcact cagcagatcg tccagctgca cgtcgcggcc
    66661 cacttcggta ttgagtttga attcaatacc catgccggtg aagatttcac ggcgacgcgt
    66721 cattacctct ttttccagct tgaaggccgg aataccgaag gtcagcagcc cgccaatttc
    66781 tggatgacgg tcgaagacaa cggcttttac gccgttacgc gtcaggacat ccgcacacgc
    66841 cagacctgcc gggcctgcgc cgataatcgc cactttttta ccggtttgct tcacgccgga
    66901 catatccgga cgccagccca tttcgaacgc tttatcattg atataacgct caatgttgcc
    66961 gatggtcacc gcgccaaact catcgttcag agtgcaggaa ccttcgcaca gacggtcttg
    67021 cgggcagact cgtccgcaaa cttccggcag ggtgttggtc tggtgcgaca gttccgccgc
    67081 ttcaaaaata cgcccctcgt tggcgagctt cagccagttc gggatgtagt tgtgtaccgg
    67141 acatttccac tcgcagtatg ggttgccgca cgacaggcag cggtcagcct gcgctttggc
    67201 ctggccttcg gaaaacggct cgtaaatttc aacaaactca attttgcgga tcttcagcgg
    67261 cttctttggc ggatcaacgc gctgcaggtc gataaattga taaacattct gactcattgt
    67321 tgctacccct tactgcgcct gcacgcgcaa ctctgctgcg ctacgactac gatgacccag
    67381 cagtgctttt acatcactgg acttcggttt aaccagcgca aatttagtgg cgaaggttga
    67441 ccagttcgcc agaatctctt caccgcgctg agagccagta tgctgaacat gctcggtgat
    67501 aagaccgcgc agatgctctt catggatcgc cagatcgtca acgcttaaga cctcgaccag
    67561 ttccgggtta acgcgtttgc ggaaatcacc gctttcatcg agaacgtaag cgaagccgcc
    67621 ggtcatgccc gcaccgaagt taacgccggt tttacccaga atacagacaa taccgcccgt
    67681 catatattca cagccgttgt cgccaatgcc ttctaccacg gtgatagcac cggagttccg
    67741 cacgccgaaa cgttcacccg cgcggcctgc ggcatacaga cgaccaccgg tcgcgccata
    67801 caggcaggtg ttgccgataa tacttgcttc atggctgcgg aaggcggaac caaccggagg
    67861 acgaatggcg attaagccgc ccgccatgcc tttaccgaca tagtcgttgg catccccggt
    67921 caggtacagt tccacgccgc ccgcgttcca cacaccgaaa ctctggcctg cggtgccgtt
    67981 gaagtacgct ttgataggat cggctgccag cccctgatcg ccgtgcgtct gggcgatata
    68041 gcctgaaagc gacgcgccga cagaacggtc ggtgttgcga atatcgaacc agaaggtttt
    68101 gctctggcgt tcatcgacaa acggtttcgc ctgttgcagc aactgcgcgt tcagcaggcc
    68161 gttatcaaac ggtgggttgt tttcggtgca gtagagtgcc ttacctggat gcggttcggc
    68221 ggtctccagc agcttcgaca gcgccagttt ttgctgtttg gcggtgaaac cgtccagttc
    68281 tttcagcagg tcggtgcgac caatcagatc caccagacgc gttacgccca gctgtgccat
    68341 cagctcgcgg gtttcacggg caataaattc aaagtaattg gtaaccttga acggcaggcc
    68401 gtgatagtgg ttcttacgca gtttgtcatc ctgagttgct acacccgttg cgcagttgtt
    68461 cagatggcaa atacgtagat atttacagcc gagcgccacc atcgggccag tgccgaagcc
    68521 gaagctttct gcgccgagaa tcgccgcttt gatgatatca acaccggttt tcaggccgcc
    68581 atcgacctgc aaacggatct tatgacgcag accattggca accagcgcct gctgggtttc
    68641 aacaagcccc agctcccacg ggcaacctgc gtatttcact gacgacagcg ggcttgcgcc
    68701 ggtgccaccg tcatagcctg cgatggtgat taaatcggca taagcttttg ccacgccagt
    68761 cgcgatggtg cctacgcccg gttcggaaac cagcttcacg gagatcatcg ctttcgggtt
    68821 aacctgcttg aggtcgaaaa tgagctgcgc taagtcctcg atagagtaga tatcgtggtg
    68881 cggcggcggg gagatcagcg tcactccggg caccgaatag cgcagtttgg cgatgtaagg
    68941 agtgactttg tcgcccggca actgaccgcc ttcgcccggc ttcgcgccct gggcgacttt
    69001 aatctgaatg acgtcggcat tgaccagata cgccggagta acgccgaagc gaccagaagc
    69061 cacctgcttg atgcgcgaca ctttgttagt gccatagcgc gccgggtctt cgccgccttc
    69121 accggagttc gagttaccgc cgatgctatt catcgcttcc gccagcgcct cgtgggcttc
    69181 cgggcttaac gcgccgatag acatcgcggc ggtatcaaag cgtttaaaca gttcgcttgc
    69241 cggttcaaca tcagcaatgt tgaccgcgtt ttcacccggc gtaattgcca gcagatcgcg
    69301 cagcgtggtt gccggacgct cattaaccag cttcgcgtat tcctgatagt cgctatactc
    69361 gccgctctgt accgcctgtt gcagcgtgcg caccacgtcc gggttgtagg cgtggtattc
    69421 gccgccgtgg acgtatttca gcaggccgcc ctggctgatg ggcttgcgcg ccagccaggc
    69481 acgtttcgac agattcagca gatcctgctg gaagtcttca aagctcgccc caccaatgcg
    69541 gctgaccgcc ccctggaagc acaggcccac tacatcatcg tgcagaccga ccgcttcaaa
    69601 cagtttcgag cagcggtaag aggcgatggt ggagatgccc attttggaca tgattttgta
    69661 caagcctttg ttgatgccgt tacggtagtt gagcatcacg gtacgataat ctttggcaat
    69721 cgcatgggtg tctaccaggc ggcccagcgt ttcataggca aggtatggat aaatagccgt
    69781 cgcgccgaag cccagcaaca cggcgaagtg gtgcggatcg cgggcgctgg cggtttcgac
    69841 gatgatgttg gcatcgcaac gcaggctttg atcgaccaga cgggtctgga tcgcgccaac
    69901 cgccatcggg gctggaaccg gcaggcgatc tttagcgata ttccggtcgg agagcaccag
    69961 cagcacggtg ccgctacgta ccattttttc ggctttgtcg cacagctctt tgactgtcgc
    70021 ttcgagcgtg gttttagtga cgtcaaaggt gatatccagc gtatctgcgc ggtagtgctc
    70081 ctctttcatc gtcgtgagct gtttgaaatc ggagtagagc agaatcggcg atttaaagct
    70141 taaacggtgc gcctggccct ctgcttcgca aaagacgttc atttcacgac cgatactggt
    70201 ggcgagcgac ataacatgcg cttcacgcag cgggtcgatt ggcgggttag tcacctgggc
    70261 aaactgctgg cggaagtagt cgtaaataat gcgcggctga ctggagagca cggcgaatgg
    70321 ggtatcatcg cccatcgaac cgaccgcttc ctgaccgttt tcgcccagta cgcgaattac
    70381 ggagtccagc tcttccgcgc tgtagttaaa ctgtttctgg tagctggcaa gcgtgtcgtc
    70441 gtccagttcg cggctaccca cttcttcatc gggcagatct tcaaacggta ccagtcggcg
    70501 gacgtttttc tccatccact ctttatatgg atggcggctt ttcagatcgt catcggtttc
    70561 tgccgagtgc agaatacgcc cactgcgggt gtcgatcacc atcagttcgc ctggcccgac
    70621 gcggcctttt tcgaccactt cgtcaggctg ataatcccag ataccgactt cagaggcgca
    70681 ggtgatgagc ttgtctttgg taatgacgta gcgtgccgga cgcagaccgt tacggtcgag
    70741 gttacaggca gcgaagcgac catcggacat cacgatgccc gccgggccat cccacggctc
    70801 catatgcatg gagttaaagt caaagaaggc acgcagttcc gggtccatat ccgggttgtt
    70861 ctgccaggcg ggtggtacta atagacgcat ggcgcggatg atatccatcc cgcctgccag
    70921 cagcagttcc agcatgttat ccatcgaact ggagtcagaa ccggtttcgt tgacgaacgg
    70981 tgcggcgtcg tgcagatcag ggatcagcgg cgtctggaat ttataggtac gcgcacgcgc
    71041 ccattggcgg ttaccggtga tggtgttgat ttcaccgtta tgcgccaggt agcggaacgg
    71101 ctgcgccagc ggccagcgcg gtacggtgtt agtggagaag cgctggtgga acaggcagat
    71161 ggccgattcc agacgcaggt ccgcaagatc cagataaaag cgcggcagat ccgccggcat
    71221 acacagacct ttatagatgt tcaccagatt cgacaggcta cagacgtaga agtctttgtc
    71281 ggcttcgaga cgcttttcaa tgcggcggcg ggcgataaac agacggcgct ccatatcgcg
    71341 cggacgccag cctgccggag cgttaacgaa aatttgctca atgtggggca gagaggagag
    71401 ggcgatttca cccagcacgc cttcgttagt ggggacatca cgccagccca caatcgacaa
    71461 ggtttcgcgt tgcagctctt cttcaacgat gcggcgtgcg gcagcggcga gttcaggatc
    71521 tttattcagg aagagcatcc cgacagcgta gttttttgct aaacgccagc cgcgctcctg
    71581 cgcaacgatg cgaaaaaagc gatccggttt ttgtaacagc aagccgcaac cgtcgccggt
    71641 tttaccatcg gcgagaatcg cgccacggtg ctgcatgcgg gccagtgcgt gtattgcagt
    71701 acgcactacc ttgtggctag gttcgccttc tatgtgggcg atcaggccga aaccacagtt
    71761 atccctctca agggatttat cgtacaacat atcgtgaacc tcccccaggc tctgcgggaa
    71821 ccccacttcc gaaccgctgc gcacgggcac agaaagagcg tggcgacggg gtttgcgtgt
    71881 catacgcgcg cctcgcattc gcccccttgt tgtccttttc gcatcggtta atacggtgaa
    71941 gaggggatct caattactgc ataaatatga tgagcaggct gctcatccag aaagcttcca
    72001 gcggatttcc aacttatcgg gaaagctgat acaggtcaaa tggcaagctt attggtacag
    72061 aaatgtgctt tagcgcaaaa ttctttatta aattactgaa attaaaatgg attttatctt
    72121 taaggctaaa agggaagtcg ttagagaaac agtcaattcc tgactgtcat aaaggcgtgt
    72181 taacccttat cctgccttga actgacgaga aaaccagcat tttatactgc cttaattggt
    72241 aatgacgttt catgctactg tttttgccta aaatccataa ggagataaaa aaatctgaca
    72301 caagataagc aaaagtgatt tatgtgatta tgcatgaaat ttcggtaatt ttgattgatt
    72361 atgcagttga taaaagcggg ccgggacgat tgatccaggt catcgccgaa ttgagctaaa
    72421 aatggcaggc tttggcctct tttcaaggcg cggtctatca gattatgcag ttacagaaat
    72481 tagtcaatat gtttggtggt gatctcaccc gtcgttatgg gcaaaaggtg cataagctga
    72541 cgctacacgg cggttttagc tgccctaacc gtgacggtac catcgggcgt ggtggctgca
    72601 cattctgtaa tgttgcctcg tttgccgatg aagcgcagca gcatcgttcc attgccgagc
    72661 aactggcgca ccaggcgaat ttagttaacc gcgctaaacg ctatctggcc tactttcagg
    72721 cgtataccag cacctttgcg gaagttcagg tgctgcgttc tatgtatcag caggcggtga
    72781 gccaggccaa tattgtcggt ttgtgtgttg gtacccgccc ggactgcgtg ccggatgcgg
    72841 tgctggatct gctttgcgaa tataaggacc agggttacga agtgtggctg gagctggggc
    72901 tacaaaccgc ccacgacaaa acactgcatc gcatcaaccg cggccatgat tttgcctgtt
    72961 atcagcgtac aacccagctg gcacgtcagc gcgggctgaa ggtgtgttcc cacttaattg
    73021 tcggcctgcc tggcgaaggg caggctgaat gtttgcaaac gctggagcga gtggttgaaa
    73081 ccggcgtgga cggcataaag ctgcatccgc tgcatattgt gaaaggcagc attatggcga
    73141 aagcctggga agcggggcgt ttgaacggta ttgaactgga ggattacacg ctcactgccg
    73201 gagaaatgat tcgccatacg ccaccggaag tgatctacca ccgcatttcc gccagcgccc
    73261 gtcgcccgac gctgcttgct cctctgtggt gcgaaaatcg atggacggga atggtcgagc
    73321 tggatcgcta tctgaatgaa catggcgtac agggatcggc gctggaacgt ccgtggatac
    73381 ctccaacgga gtaggtcgtt gaggggaatt ccgcatttct cacacaattt ataacgtaac
    73441 tgtcagaatt gggtattatt ggggcaggtt gtcgtgaagg aattccctaa tgaagcaaat
    73501 tcgtctgctg gcgcagtatt atgttgacct gatgatgaag ttaggtctgg tgcgcttctc
    73561 aatgttgctg gcgctggccc tcgtcgttct tgccattgtg gtacaaatgg cggtaaccat
    73621 ggtgctgcat ggtcaggtcg aaagcattga tgttattcgt tctatcttct ttggtttgct
    73681 gattacgccg tgggcggtct actttctatc ggtggtcgtc gagcaactgg aggagtcacg
    73741 acaacgtctg tcacggctgg tgcaaaaact ggaggagatg cgcgagcgcg atttgagcct
    73801 caacgttcag ttaaaagata atattgccca gctaaatcag gaaatcgccg ttcgtgaaaa
    73861 agcggaagca gaactgcagg aaaccttcgg ccaactgaaa attgaaatca aagagcgcga
    73921 agagacacaa attcagctcg agcagcaatc ctcattctta cgttccttcc ttgatgcttc
    73981 acccgacctg gttttttatc gtaacgaaga taaagagttt tccggctgta accgcgcgat
    74041 ggagctgctg accggaaaaa gcgaaaaaca actggttcac ctgaaacctg ctgatgttta
    74101 ctcaccggaa gccgccgcaa aagtcattga aaccgatgaa aaagtgttcc gtcataatgt
    74161 gtcactgacc tatgaacagt ggctggatta cccggacggg cgcaaagcct gctttgaaat
    74221 ccgtaaagtg ccgtactacg accgcgtggg taaacgtcac ggtttgatgg gctttggtcg
    74281 cgacattacc gagcgtaagc ggtatcagga tgcgcttgaa cgggccagcc gcgacaaaac
    74341 gacgtttatc tccaccatca gtcacgaatt gcgtacaccg ctgaacggta tcgtcggcct
    74401 gagccgtatt ctgctggata ccgaactcac cgccgagcag gaaaaatatc tcaagaccat
    74461 ccatgtttcg gccgtcacgc tggggaatat ctttaacgat attatcgaca tggataagat
    74521 ggaacggcgc aaggtccagc ttgataatca accggttgat ttcaccagct tccttgccga
    74581 tctggaaaat ctctccgcat tgcaggcgca acaaaaagga ttgcgcttta acctggagcc
    74641 gacgctgcca ttaccgcatc aggtcattac cgacgggacg cgtttacggc agatcctgtg
    74701 gaacctcatc agtaacgccg tcaaattcac ccagcaaggc caggttaccg tgcgcgtgcg
    74761 ctacgatgaa ggcgatatgc tgcattttga agtggaagac tctggtatcg gcattccgca
    74821 ggatgagctg gataaaattt tcgccatgta ttaccaggtg aaagacagtc atggcggtaa
    74881 acctgccacc ggcaccggta ttggtctggc cgtttctcgt cgtctggcga aaaatatggg
    74941 cggcgatatt acggttacca gcgaacaggg caaaggttca acctttacgt tgacgatcca
    75001 cgcaccgtcg gtagcagaag aggtcgatga tgcgtttgat gaagacgata tgcctttacc
    75061 ggcgctgaat gtgctgctgg tggaagacat tgaactgaac gtgattgttg cgcgttctgt
    75121 gctggaaaaa ttaggtaaca gcgttgatgt cgccatgacc ggcaaggcgg cgctggagat
    75181 gtttaaaccg ggcgaatacg acctggtgtt gctggatatt cagttgccag atatgaccgg
    75241 gctggatatc tctcgtgaac tgacgaagcg ttatccgcgc gaggatttac cgccgctggt
    75301 ggccttaacc gctaacgtgc tgaaagacaa acaagagtac ctcaatgctg gaatggatga
    75361 tgtgctgagt aagccgcttt cggttccggc gctaaccgcg atgatcaaga aattctggga
    75421 tacccaggat gatgaggaga gtacggtgac gacagaagag aacagtaaat cagaagcatt
    75481 gctcgatatt cccatgctgg aacagtatct cgaacttgta ggaccgaagc tgatcaccga
    75541 cgggttagcg gtgtttgaga agatgatgcc gggctatgtc agcgtgctgg agtcgaatct
    75601 gacggcgcag gataaaaaag gcattgtaga ggaaggacat aaaattaaag gtgcggcggg
    75661 gtcagtgggg ttacgccatc tgcaacagct gggtcagcaa attcagtctc cggaccttcc
    75721 tgcctgggaa gataacgtcg gtgaatggat tgaagagatg aaagaagagt ggcgtcacga
    75781 cgtagaagtg ctgaaagcgt gggtggcaaa agccactaaa aaatgacccc ggctagaccg
    75841 gggtgcgcga atactgcgcc aacaccaggg aaatcttggc tgcgccgtaa attattatga
    75901 tgagttacaa gggcacagca ctgtttttca ggccgcacgc agttaagata gcaaaactta
    75961 aatgatttgt tacatgaatc agttaaatgt gtgatgcgta ccatttttat taaaaaaatt
    76021 aatgtgtttc agtcaattgc gaaaggatga tcacaatgaa gaaaattggc gtaattctga
    76081 gcggatgcgg cgtctatgac ggttctgaaa ttcatgaagc ggtgttgacg ttgttagcta
    76141 tttcacgcag cggtgcgcag gcggtctgct ttgcaccgga taagcagcag gttgatgtta
    76201 tcaaccattt aactggcgaa gcgatgacgg aaacgcgcaa cgtgctgatt gaagcggcac
    76261 gaataacgcg cggtgaaatc cgtcctctgg cccaggccga tgccgctgaa ctggatgcgt
    76321 tgattgtgcc gggggggttt ggcgcggcga agaatttaag caattttgcc actcttggta
    76381 gcgaatgcac cgttgaccgt gaattaaagg cgctggcaca agcgatgcat caggccggaa
    76441 aaccgcttgg ttttatgtgt attgccccgg cgatgctgcc gaaaattttc gatttcccgc
    76501 tgcgtttgac catcggtact gatatcgata ccgcagaagt gctggaagag atgggcgcgg
    76561 agcatgtgcc ctgtcctgtc gatgatatcg tggttgatga agacaataag attgtcacca
    76621 ccccagcata tatgctggcg cagaacattg cagaagcggc gagcggcatt gataagctgg
    76681 tttcccgcgt gctggttctg gctgaatgag taaaagccgc ttaacggtgt ttagtttcgt
    76741 tcgccgtttt cttttgcggt taatggttgt cctcgccgtt ttctggggcg ggggcatcgc
    76801 gttgtttagt gttgcgcctg ttcccttctc agcggtaatg gtcgagcgac aggtcagcgc
    76861 ctggctgcat ggcaattttc gttacgtggc acattctgac tgggtcagta tggatcaaat
    76921 ctcgccgtgg atgggactgg cggtgattgc cgcagaagat cagaaatttc ctgagcactg
    76981 gggctttgat gtcgcttcca ttgagaaagc cctggcgcac aacgagcgca atgaaaaccg
    77041 tattcgcggt gcttcaacga tttctcagca gacggccaaa aatctctttt tatgggatgg
    77101 gcgtagctgg gtacgaaaag ggctggaagc tggattaacg ctggggatag aaacagtctg
    77161 gagcaaaaag cgtatcctga cggtttacct gaatatcgcc gaatttggcg acggtgtgtt
    77221 tggcgtcgaa gctgcggcac aacgttattt ccacaaaccc gcgagcaaac ttacccggtc
    77281 ggaagctgca ttactggcag ctgtattacc taatccactt cgtttcaaag tctcctcgcc
    77341 atcgggctac gtgcgtagcc gtcaggcgtg gattttacgg cagatgtatc agttaggcgg
    77401 tgagccgttt atgcaacaac accagctgga ttaagcttcg cgcgaagtga gggccagcgt
    77461 tttgctctca cgaggttgca gatctttctg cagggcaata gccagcgccg gttgggcgat
    77521 aaatcgtttc cataaccgct cttgcttacg cagacaacac cacttcgacc aggtcgccag
    77581 tggaatcaat gtgctttcac caatgttgtc acagacgacc gggataacct ctgactcact
    77641 gatgcggtga cagaggatat tctgcggctt cagcgacatc gtcacgatac ggttatcctg
    77701 caaataacgc ttcagctgtt tcagtagctg gcgcagttgt gcaatgtctt cttcgtaacg
    77761 gcattgttca gcaaattcgg tcagcgtaat ggagggtttg ccgtcaaaat ctgcaatcac
    77821 atcgtagaca taaccggttc cgcaatccgt ctcaacggta ccgtgatagc gcggtattcc
    77881 actccagtct tttaaccggc gaccaagatg cgcgtagtac tttaactcac ggcggatctc
    77941 tttatcgcca ccatcgccac gatggtagac aatcttgata cagcgttggg catcttccgg
    78001 atgcgcataa catttccgat gtcttccggt gcccagggga ctttgttcag ataagcgaat
    78061 catgccatct cctggaaata ttttctgacg atctccagta tggtcgatta acttaaacaa
    78121 aacctaaaca atgcctctta aggttttctt aaggttcttc tgaaagtgaa aagcgcacaa
    78181 aatgtacaaa tgacgttgat tgagaacgat ttcagtacgt gattttctta ttgtcggggg
    78241 gagtttgaag ggagttgtat gtcaaagtga tgaagattaa tcttcatcaa aaccagagtt
    78301 aaagagggcg ataacggcgg ccagtgcttc ctcttcctgt gggccggtcg cttcaacttc
    78361 aatctgccgt cctttggcag aatccaacat cagcagcgca ataacgctgt tggcttcggc
    78421 ctcggtgcct tcgtcattac gtaacagcac ttcagcgtca aaaccctgca ttaattcaaa
    78481 cagcttcatt gcaggccggg catgcatgcc cagcttgttt gtgatttcaa cagtttgctt
    78541 gacggtcatg gtttacgttt ttccagcgta cgatggcgtg actggacgtt tttaccgcgc
    78601 gagcggaagt agtctgccag ttgctctgca atatacaccg aacggtgctt tccgccggta
    78661 caaccaatgg cgaccgtcaa gtagctacgg ttgttggttt ccagcatagg taaccatagt
    78721 tcaagatagc ttcgcgtctg gtagataaaa ttgtgtactt ctgtgtggcg gtcgaggaac
    78781 gcggcgacag gtttatcaag acctgtcatt ggacgcagtt tcggatccca gtgcgggttc
    78841 ggcaagaagc gcacgtcaaa gacgtaatct gcatcgatag ggataccgtg tttgaagccg
    78901 aaagactcaa agaccatggt cagttcgcgt tcacgtttac ccagcagacg ggtacgcagc
    78961 atttcagcca gctcgtgaac ggacatttct gaggtatcga caatcagatc cgctcgcgaa
    79021 cgcagaggct ccagcaaatc gctttctttg tcgatagcac tttccagcga caggtttttg
    79081 ctggaaagcg gatgcagtcg gcgcgtgtca ctgtaacgac gaattaaggt attacggtcg
    79141 gcatccagga acagtagttg cggtgagaaa gcgtcaggca ggttactcat cgcctgttcg
    79201 aatatttctg gtgactccgg catattacga acatcaatgc tgacggcggc agaaatctcg
    79261 cgatcggcca gagttcgagc cagatcgggt aacaacacta cgggaaggtt atccacgcgg
    79321 taaaaaccca tatcttccag cgcacgcagg gcgacagatt tacctgaacc tgaacgtccg
    79381 ctgacgatca tcagtaccat gtactgtttc tcctcacaac gtctaaaaga gacattaccg
    79441 aataactacg cttcatccgg agtaccttcg gtatccgtaa tgatttgata cagctcttca
    79501 tcgctctggg ctgcgcgtaa acggcggcag atggttttgt ccgccagacg tttcgccacc
    79561 agcgacagag tatgcaggtg cgttttagtt tggtctgccg gcaccagcag ggcaaaaagt
    79621 aagtccaccg gttggttgtc gatggcatcg aaagctatag gcgtttcgag ctgaacgaaa
    79681 acgccaacgg cgcgcagagt atcttcttcc agtttgccat gcggaatggc aataccattg
    79741 ccgataccgg tactgcccat tttttcccgc gtcaggatag cttcaaaaac cacctgaggc
    79801 ggcaggctaa gttgtttcgc cgccagctcg ctgatgattt ccagggcgcg ttttttgctc
    79861 tgacagtgga cgcggcttcg cgtacattcc ctgttaagaa cactgctaag ctgtagagtt
    79921 gtatcattat ttgtcataat ttcacctaag aacctgccca ctcaaacagt ggaacaggct
    79981 cggagcatta accgtacaaa tggcccgttg tgccgcacaa caggccgcca tgcacatgct
    80041 aattgcccgg acaattagtg ttgtttcagt ttatctttat gtttggtcaa ctggcgtgcc
    80101 agcttatcaa ttaagccatc aatggcagcg tacatgtcct gaccttccgc gctggcatga
    80161 atttcaccac cgtttacatg cagtgttgca tctgaggtgt gggtgacttt ctccactttc
    80221 agaacaacat agacctggtt gattcggtca aaatattgct caagtttggc aaatttggct
    80281 gtaacaaatt cgcgcagtgc ctcggtgatc tcgacgttat ttccggtaat gttgagctgc
    80341 atagtgtctt ccttatcggt tgggtcaaac gagctgttta cgctggtttg acggcggaat
    80401 ggataaagac tctcggtact tcgcaacagt gcggcgtgcc accatgatac cttgttccga
    80461 cagcaaagag gttaacttgc tgtcgctcaa cggtttcgct gggttttccg ccgcgattaa
    80521 tttcttcacc agcgcacgaa tcgccgtgga ggaagcttcg ccgccgccct cggtattgac
    80581 gtgactggaa aagaaatact tcagttcaaa aatgcctcgt ggactatgca ggtatttttg
    80641 cgtggtcacg cgagatatcg tcgattcatg catttcgaca gcctgggcga tatcggccag
    80701 taccatcggt ttcatatatt cttcgccttg ctcaaagaag gcttgctgct gttcaacgat
    80761 acagcgactc acgcgcagta gcgtatcgtt acggctttcc agactcttga tcaaccattt
    80821 ggcatcctgc agattgctgc ggataaactg gctgtcacca tcattgcgag cgttattgca
    80881 catcgaggcg tagtgctggt tgatttgcag acgcggaatg ctgtcactgt tgagttctac
    80941 tgtccagtga ccgttatgct tacgcaccag cacatctgga atgacatact caggttcgcc
    81001 agtctggatc gactgcccgg ggcgcggatc gagcgactgg atcagattga cggcttcttt
    81061 cagcacatct tctttcagac gcgtgacgcg cattaaagtg cggaagtcgt gattggctaa
    81121 cagatcgaga tgatcgctaa tgatcaatct ggcctcttcc agccacggcg tggttttatc
    81181 gaattgggag agttggatta gcaggcagtc acgcagatct tttgccgcta caccgaccgg
    81241 atcaaaccgt tggatccgct taaggacggc ttcaacctcg tcgatgtcga tctcttcatc
    81301 gcccatactt tcgagaatat cttccagcgg gacagtcaga taaccggtgt catcaacggc
    81361 atcgacgata gaggtagcaa tagcgcggtc agtgtcggaa aacggtgtca gctcgacctg
    81421 ccacatcagg taatcctgca aggtctgcgt cgtttcgccc tgatagaccg gcagctcatc
    81481 gtcaatgtag tcaccgctgg tgccggatgg tgtaccagcg gtgtaaatgg tgtcccaact
    81541 ggcatcgagc ggcagctctt ccggcatctc tttttgttcg agcgcgtcgg cggtgtccag
    81601 cgtttcactg tcttgcgttt cgcgggtgtc gatttcttca tgagtgtcga tttgctcaag
    81661 cagcggatta ctctccagcg cctgctgtag ctcctgctga agttccagcg tcgacaactg
    81721 caacagacga attgcctgtt ggagctgtgg cgtcatcgcc agttgttggc taagcctgag
    81781 ttgcaaacct tgcttcatgt tcagaatcgt actctcctgc taaaacgtcg caaacttcta
    81841 ccctatcaga gtctgaagtc ttccccaagg tatacacgct taacgtgttc gtcttgtaag
    81901 atttctgtag gcgtgccgtg ggcgatcaaa tgcccctgac tgacgatata agcgcgttca
    81961 caaaccgcca gtgtttcacg cacgttgtgg tcagtgatca gcacgcccag gccgctgtcg
    82021 cgcaggtgct caatgatgcg tttaatgtcg ataaccgaga tcgggtcaac accggcaaac
    82081 ggttcgtcga gcagaataaa tttcggattc gcagccagtg cgcgggcaat ttctacacgg
    82141 cgacgttcac ccccggagag tgactgcccc atgctgtcac gcaggtgctc aatgtgaaac
    82201 tcttccatca gctcgttcgc gcggtcttca cgttgttcag cagacaagtc gtcacgaatt
    82261 tgcagtaccg ccatcaggtt atcgtaaacg ctgaggcgac ggaaaatgga ggcttcctgt
    82321 ggcagatagc cgataccgcg gcgcgcgcgt gcatgcagag gcagcagact gatatcgtca
    82381 tcatcaataa tgatgttgcc cgcatcgcgc ggcacaatgc ctacaaccat atagaaagtg
    82441 gtggtcttac cggcaccgtt tggccccagc agaccgacaa tttccccgga gttgacggtc
    82501 aggctgacgt cttctaccac gcgacggcct ttataggctt ttgcaaggtt ctttgcagtt
    82561 aatgttgcca taacgaatta attacccttc ttctgtgccg gggtctggcc tttgttgttt
    82621 ttatcctgca gctgcgacgg caccagaacg gtggttacgc gcttgccttt gtcgctgaaa
    82681 gcctgcattt tctgctcttt taccaggtaa gtgatcttat cgcccttaat gttgctatcg
    82741 acctgctgca gataagcatt acccgtcaga acgacaaaat cttttgccag ttcgtagtgc
    82801 atctgggaag cgtgaccttc aacgggttta ccgttgtcct gcatctggta gaacgttgcc
    82861 ggtttaccgt agccgtcaat cacttcttta ccttgttcgc cgcccggacg ggtaacgacc
    82921 actttgtcgg cattaatttt gatggtgccc tgggtgacga tgacattacc ggtaaaggta
    82981 accacgttgc cttgcatatc aagagattgc tggtccgatt caatgtggat cggctgatca
    83041 gtgtctccgg ttacggcaaa tgccggaatg ctggcggcca gaagtgagct ggcaagcaca
    83101 agattaaggc tgagtttgtt tgttttgaat ttcataggat gttctaacct tttcaatcag
    83161 ctcggcgttc ttgctgcgta agttgccgcg cattttcaga ccgctggagt taaatgttgt
    83221 tccgtataac gtgacgaggt cttcagaggt aacatcctga gtcaccagat tgatctgcgc
    83281 gttatccgtc gtgattctgc gaagttgaga gtctggcaca agtgcgttga cttcaacgtg
    83341 tccatataaa tagagcatcc ggtcattggt cagcttggct ttatctgctt ttacggacca
    83401 tgtcgggatt ttatccttat caaacgtggt aagtaccggc tgcgtaaacc acgaaacggc
    83461 ctgatcggaa taatattcaa cgtgttgagc aatcaatcga tagcttagtg ccccttctgg
    83521 gttatagacg agcgtgtccg tatgctcgct tttataggtg ggatcattgt tgttgacgac
    83581 cacctgggcg gtatcgtctt tttcggccat attaatgccg atcatcacca gaaccgccag
    83641 tgatagcaca atgataaccc aacgtctggc tttactcata tcgattgccc tttggcttca
    83701 tccagtttgc cctgcgccag gagtaataag tcgcaaactt cgcgcactgc gccacgaccg
    83761 ccagcaatgc gcgtcacgta atcggcgcgc gggatcaaca gtggatgcgc atcggccacg
    83821 gcgacgctta aacccacttt ttccattacc ggccagtcga tgagatcatc gccgacataa
    83881 gccacatttt ccggggcaat cgccagtttt tccagcaaat cgctaaaggc gatcagtttg
    83941 tttgactgcc cctgatacaa gtgagtgatc cccaatgtgg cacaacgatc ttctaccagt
    84001 ttagcctttc gcccggtaat gatagcgact tcaatatcag aggtgagcgc acaacgaatg
    84061 ccataaccgt cacgaacatt gaacgctttc agctcttcgc cgttattgcc catataaatc
    84121 aggccatctg acagtacgcc atcaacgtcg aggatcagca gacgaatgtt ctctgctttt
    84181 gccataacgt cggcgctgac agggccgtaa caggtcgcaa gcgacgcacc tgctttgctc
    84241 attgttgttt atccttgaat ctttacacta cgcctgcacg cagtaaatca tgcatatgta
    84301 acacaccgag taaatggtcg ccatcggcaa ccatcacgga ggtgatatgg cgggactgca
    84361 ttaagttcag tgcctcaacg gccagaatgc cagggcgcac acgtattccc cccggcgtca
    84421 tcacatcggc aatacttaac tgacgaacat ccacgcccat atcgaagaca cggcgtaaat
    84481 caccgtcggt aaagatgcct tcaatcatca tattgtcatc gcaaatgaca gtcataccaa
    84541 gatttttgcg ggta
//
LOCUS       DAMZET010000005        77329 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.005, whole genome shotgun sequence.
ACCESSION   DAMZET010000005 DAMZET010000000
VERSION     DAMZET010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 77329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.005"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..384)
                     /locus_tag="POB34_000398"
     CDS             complement(<1..384)
                     /locus_tag="POB34_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HDI5917407.1"
                     /translation="MKNRNRMIVNCVTASLMYYWSLPALAEQSSSEIKIVRDEYGMPH
                     IYANDTWHLFYGYGYVVAQDRLFQMEMARRSTQGTVAEVLGKDFVKFDKDIRRNYWPD
                     AIRAQIAALSPEDMSILQGYADGMNA"
     gene            complement(736..900)
                     /locus_tag="POB34_000399"
     CDS             complement(736..900)
                     /locus_tag="POB34_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HDI5917408.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQARRVLQQMSDERLR
                     DIGLRREDVE"
     gene            1077..2489
                     /locus_tag="POB34_000400"
     CDS             1077..2489
                     /locus_tag="POB34_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDI5917409.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGISISTVQ
                     QAYQTLETMKFITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DNSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQTYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLYRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGDAVYRAME"
     gene            complement(2732..3688)
                     /locus_tag="POB34_000401"
     CDS             complement(2732..3688)
                     /locus_tag="POB34_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDI5917410.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVILLVTECANDSQITALLNYIL
                     LTGDEARFKKFISELTRRMPQHRERIMTIAERIYNDGWLLGRERGRKEGKVEGERSLL
                     RLLLQNGADPEWIQRYTGLSAEQMQALDQPLPESKREPWIEY"
     gene            4155..5387
                     /gene="mdtM"
                     /locus_tag="POB34_000402"
     CDS             4155..5387
                     /gene="mdtM"
                     /locus_tag="POB34_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HDI5917411.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPLQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            5428..6708
                     /locus_tag="POB34_000403"
     CDS             5428..6708
                     /locus_tag="POB34_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HDI5917412.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLSNF"
     gene            6824..7975
                     /locus_tag="POB34_000404"
     CDS             6824..7975
                     /locus_tag="POB34_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HDI5917413.1"
                     /translation="MSLITDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            7985..8752
                     /locus_tag="POB34_000405"
     CDS             7985..8752
                     /locus_tag="POB34_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917414.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            8749..9006
                     /locus_tag="POB34_000406"
     CDS             8749..9006
                     /locus_tag="POB34_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HDI5917415.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            9071..9931
                     /locus_tag="POB34_000407"
     CDS             9071..9931
                     /locus_tag="POB34_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HDI5917416.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNR
                     FYRFTPKSSH"
     gene            9999..11177
                     /locus_tag="POB34_000408"
     CDS             9999..11177
                     /locus_tag="POB34_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5917417.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFAFSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMNWWLLAIL
                     YGLAGFGYIIVATFLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(11190..11744)
                     /gene="kptA"
                     /locus_tag="POB34_000409"
     CDS             complement(11190..11744)
                     /gene="kptA"
                     /locus_tag="POB34_000409"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HDI5917418.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            11994..12677
                     /locus_tag="POB34_000410"
     CDS             11994..12677
                     /locus_tag="POB34_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917419.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            12674..13135
                     /locus_tag="POB34_000411"
     CDS             12674..13135
                     /locus_tag="POB34_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDI5917420.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            13148..14320
                     /gene="iadA"
                     /locus_tag="POB34_000412"
     CDS             13148..14320
                     /gene="iadA"
                     /locus_tag="POB34_000412"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDI5917421.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPP
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            14385..15296
                     /gene="hypT"
                     /locus_tag="POB34_000413"
                     /gene_synonym="qseD"
     CDS             14385..15296
                     /gene="hypT"
                     /locus_tag="POB34_000413"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HDI5917422.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     GAPFDHIRLFESQLFPVCASDEHREALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(15289..15681)
                     /gene="iraD"
                     /locus_tag="POB34_000414"
     CDS             complement(15289..15681)
                     /gene="iraD"
                     /locus_tag="POB34_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HDI5917423.1"
                     /translation="MMRQSLQAVLPEISGNKTSPLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDESLTFDLVWDNGGWRSATLENVS"
     gene            complement(15678..15761)
                     /gene="idlP"
                     /locus_tag="POB34_000415"
     CDS             complement(15678..15761)
                     /gene="idlP"
                     /locus_tag="POB34_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HDI5917424.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            16354..17184
                     /locus_tag="POB34_000416"
     CDS             16354..17184
                     /locus_tag="POB34_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HDI5917425.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPKVDNELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIGMGN
                     DPIIGIEAYPGTAEIHAKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(17325..18098)
                     /gene="uxuR"
                     /locus_tag="POB34_000417"
     CDS             complement(17325..18098)
                     /gene="uxuR"
                     /locus_tag="POB34_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HDI5917426.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(18313..19773)
                     /locus_tag="POB34_000418"
     CDS             complement(18313..19773)
                     /locus_tag="POB34_000418"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HDI5917427.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(19854..21038)
                     /gene="uxuA"
                     /locus_tag="POB34_000419"
     CDS             complement(19854..21038)
                     /gene="uxuA"
                     /locus_tag="POB34_000419"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDI5917428.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            21378..22721
                     /gene="gntP"
                     /locus_tag="POB34_000420"
     CDS             21378..22721
                     /gene="gntP"
                     /locus_tag="POB34_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HDI5917429.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(22964..23866)
                     /gene="fimH"
                     /locus_tag="POB34_000421"
     CDS             complement(22964..23866)
                     /gene="fimH"
                     /locus_tag="POB34_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HDI5917430.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(23886..24389)
                     /gene="fimG"
                     /locus_tag="POB34_000422"
     CDS             complement(23886..24389)
                     /gene="fimG"
                     /locus_tag="POB34_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HDI5917431.1"
                     /translation="MKWCKRGYVLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(24402..24932)
                     /gene="fimF"
                     /locus_tag="POB34_000423"
     CDS             complement(24402..24932)
                     /gene="fimF"
                     /locus_tag="POB34_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HDI5917432.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(24942..27578)
                     /gene="fimD"
                     /locus_tag="POB34_000424"
     CDS             complement(24942..27578)
                     /gene="fimD"
                     /locus_tag="POB34_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HDI5917433.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGVQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCI
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(27645..28370)
                     /gene="fimC"
                     /locus_tag="POB34_000425"
     CDS             complement(27645..28370)
                     /gene="fimC"
                     /locus_tag="POB34_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313302.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDI5917434.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILIFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKRENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGARVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(28407..28946)
                     /locus_tag="POB34_000426"
     CDS             complement(28407..28946)
                     /locus_tag="POB34_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDI5917435.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLQECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(29011..29559)
                     /gene="fimA"
                     /locus_tag="POB34_000427"
     CDS             complement(29011..29559)
                     /gene="fimA"
                     /locus_tag="POB34_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDI5917436.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALALDGATFSSETTLNNGTNTIPFQARY
                     FATGAATSGAANADATFKVQYQ"
     gene            complement(30040..30636)
                     /gene="fimE"
                     /locus_tag="POB34_000428"
     CDS             complement(30040..30636)
                     /gene="fimE"
                     /locus_tag="POB34_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HDI5917437.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(31114..31716)
                     /gene="fimB"
                     /locus_tag="POB34_000429"
     CDS             complement(31114..31716)
                     /gene="fimB"
                     /locus_tag="POB34_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HDI5917438.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            33170..33886
                     /gene="nanC"
                     /locus_tag="POB34_000430"
     CDS             33170..33886
                     /gene="nanC"
                     /locus_tag="POB34_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HDI5917439.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            33906..35012
                     /gene="nanM"
                     /locus_tag="POB34_000431"
     CDS             33906..35012
                     /gene="nanM"
                     /locus_tag="POB34_000431"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HDI5917440.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGDLYVFGGIGKNSKGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGDNLKWNRLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGESAGGKAVTDSVLISVKDNKVTVQN"
     gene            35076..36056
                     /locus_tag="POB34_000432"
     CDS             35076..36056
                     /locus_tag="POB34_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HDI5917441.1"
                     /translation="MNAIISPNYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFTHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPHNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQFHSQLNNIT
                     AAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYFGSAYRSAENWTTSLRSSHFSAAARRGIISDRFVEAILQFWRER"
     gene            complement(36639..37865)
                     /locus_tag="POB34_000433"
                     /pseudo
     CDS             complement(36639..37865)
                     /locus_tag="POB34_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378101.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            37943..38242
                     /locus_tag="POB34_000434"
     CDS             37943..38242
                     /locus_tag="POB34_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5917442.1"
                     /translation="MLGVLTLARDIQLAIVNKMTQVLIGDIYLRQQCDVFWLGYTISP
                     AYTRQGYAIEAITATIDWIKENGFSLIKAGVNPENTLSKKLLIRIGFNFSSIEDG"
     gene            38619..38876
                     /locus_tag="POB34_000435"
     CDS             38619..38876
                     /locus_tag="POB34_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjhX family toxin"
                     /protein_id="HDI5917443.1"
                     /translation="MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLT
                     DCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRAQLDNR"
     gene            38888..39439
                     /locus_tag="POB34_000436"
     CDS             38888..39439
                     /locus_tag="POB34_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014837489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI5917444.1"
                     /translation="MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDES
                     ARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMHILAPLAVIPEYQGMGVGGRLIR
                     TGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
                     QPMNVTGHIQCAQALMKPEHWRE"
     gene            39491..40237
                     /locus_tag="POB34_000437"
     CDS             39491..40237
                     /locus_tag="POB34_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDI5917445.1"
                     /translation="MDIPRIFTISESEHRIHNPFTEEKYATLGRVLRMKPETRILDLG
                     SGSGEMLCTWARDHGITGTGIDMSSLFTAQARRRAEELGVSERVHFIHNDAAGYVANE
                     KCDVAACVGATWIAGGFAGTVELLAQSLKPGGIMLIGEPYWRQLPATDEIAQACGVSS
                     TTDFLTLPGLVSAFDDLGYDVVEMVLADQEGWDRYEAAKWLTMRRWLEANPDDDFAAE
                     VRAELNISPKRHVTYAREYFGWGVFALIAR"
     gene            40365..40625
                     /locus_tag="POB34_000438"
     CDS             40365..40625
                     /locus_tag="POB34_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917446.1"
                     /translation="MSQHEQFCQACGMPMSAPDAHSASDQYCAYCSYSNGNLKPWEEA
                     VSGLASFLDSWQKVGPEEAHKRAKRYLTAMPAWAHKAESDLS"
     gene            40663..40779
                     /locus_tag="POB34_000439"
                     /pseudo
     CDS             40663..40779
                     /locus_tag="POB34_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227376.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            41024..42145
                     /locus_tag="POB34_000440"
     CDS             41024..42145
                     /locus_tag="POB34_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDI5917447.1"
                     /translation="MSFSVQETLFSLLQHNAISGHENAVADVMLCEFRRQAKEVWRDR
                     LGNVVARYGSDKPDALRLMIFAHMDEVGFMVRKIEPSGFLRFERVGGPAQVTMAGSIV
                     TLTGDKGPVMGCIGIKSYHFAKGDERTQSPSVDKLWIDIGAKDKDDAIRMGIQVGTPV
                     TLYNPPQLLANDLVCSKALDDRLGCTALLGVADAISTTELDIAVYLVASVQEEFNIRG
                     IVPVLRRVKPDLAIGIDITPSCDTPDLHDYSEVRINQGVGITCLNYHGRGTLAGLITP
                     PRLIRMLEQTALEHNIPVQREVAPGVITETGYIQVEQDGIPCASLSIPCRYTHSPAEV
                     ASLRDLTDCIRLLTALAGMSAAHFPVEPDSGTTQEAHPL"
     gene            42142..42420
                     /locus_tag="POB34_000441"
     CDS             42142..42420
                     /locus_tag="POB34_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDI5917448.1"
                     /translation="MKKILVACGTGMSTSTMIAHKLQEFLTEQGISATTAQCCLNEIP
                     LNCNGMDLIVTSMRTNSDYGIPTLNGAALLTGINDDALKQQIKALLTQ"
     gene            42432..43745
                     /locus_tag="POB34_000442"
     CDS             42432..43745
                     /locus_tag="POB34_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDI5917449.1"
                     /translation="MFDYILSLGGTVFVPIIMIVIGLIFRIPWLQAIKAGVTVGIGFV
                     GMGLVIVMAIDSLSPPIKVMIERFGLALHVFDVGAGPASGVGYATAIGAMIIPVIFLL
                     NVAMLVTRLTKTMNVDIYNYWHYAITGTVVQLMTGSLIYGVLGAICHAALSLKMADWT
                     AKRVQNIVGLEGISIPQGYGSSSVPLFVLLDAIYEKIPFMKGRNIDAQEIQKRYGMVG
                     DPVIIGVVLGLIFGLAAGEGFKGCASLMITVAAIMVLFPRMIRLIVEGLMPISDGARK
                     FFQKYFKGREVYIGLDTAVTLGHPTTIAVGLLLIPIMLILASILPGNKVLPLADLPVA
                     PFFICMATVIHRGDLVRTLISGVIVMITVLLIATQFAPYFTEMALKGGFSFAGESAQI
                     SALSVGNMFGWSISELMSLGIIGVVVAVGIVASVVLFLRKRELSE"
     gene            43758..44564
                     /gene="sgcQ"
                     /locus_tag="POB34_000443"
     CDS             43758..44564
                     /gene="sgcQ"
                     /locus_tag="POB34_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA family protein SgcQ"
                     /protein_id="HDI5917450.1"
                     /translation="MSWLKEVIGTEKAVIAMCHLRALPGDPSFDAQLGMNWVIDKAWD
                     DLMALQNGGVDAVMFSNEFSLPYLTKVRPETTAAMARIIGQLMSDIRIPFGVNVLWDP
                     VASFDLAMATGAKFIREIFTGAYASDFGVWDTNVGETIRHQHRIGAGEVKTLFNIVPE
                     AAVYLGNRDICSIAKSTVFNNHPDALCVSGLTAGTRTDSALLKRVKETVPDTVVLANT
                     GVCLENVEEQLSIADGCVTATTFKKDGVFANFVDQARVSQFMEKVHHIRR"
     gene            44695..45126
                     /locus_tag="POB34_000444"
     CDS             44695..45126
                     /locus_tag="POB34_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDI5917451.1"
                     /translation="MINDIKWVQAQREATDWRQAVEIATRPLVAYGAAQPCYVNGIIE
                     NTLNWGPYYLIAPGIALPHARPEQGANYNQVSITTLRTPVAFGNEECDPVWLLLCVSA
                     TDANAHILTIQRISQFIDSPQRLAAVGNASTDDALFALVSG"
     gene            45138..45770
                     /locus_tag="POB34_000445"
     CDS             45138..45770
                     /locus_tag="POB34_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3 epimerase family protein"
                     /protein_id="HDI5917452.1"
                     /translation="MILHPSLASANPLHYGRELTALDNLDFGSLHLDIEDSSFINNIT
                     FGMKTVQAVARQTPHPLSFHFMVARPQRWFNALAEIRPAWIFVHAETLDYPSETLTEI
                     RHTGARAGLVFNPATPIDAWRYLASELDGVMVMTSEPDGQGQRFIPSMCEKIQKVRTA
                     FPQTECWADGGITLAAAQQLAAAGAQHMVIGRALFSSSDYRATLAQFATL"
     gene            45787..46569
                     /locus_tag="POB34_000446"
     CDS             45787..46569
                     /locus_tag="POB34_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDI5917453.1"
                     /translation="MSQQRPDRIKQMLHYLWQHRHLSTQQAMELFGYAEATVRRDFQY
                     IANQYPGMIRGHGCLDFDDSTDDKEYVFDVKRTLQSVAKREIAALARTMIKDGDCFFL
                     DSGSTCLELAKCLADARVKVICNDIKIANELGCFPHVESYIIGGLIRPGYFSVGESLA
                     LEMINAFSVERAFISCDALSLETGITNATMFEVGVKTRIIQRSREVILMADHSKFDAV
                     EPHAVATLSCIKTIISDSGLPETIAQRYQRAGCQLFLPHSIK"
     gene            46872..47660
                     /locus_tag="POB34_000447"
     CDS             46872..47660
                     /locus_tag="POB34_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDI5917454.1"
                     /translation="MVRKGCNSLVRAEKILTHIAWVGMASYMELLNKFQYPKSSLLNL
                     LNVMVECGFLIKNKNGYYSLGIKNYELGCQALHRQNIFEVTKRPMQELSLKSGLVCHL
                     GAMESISAIYLDKIESPDSVPTSKSWIGKKLELHITALGKALLAWKTREELDYFLEAL
                     TLTPHTRNTFTDKKLFLEELQKTRLRGWAIDNEESTYGAVCLSMPVFNMYNRVNYAIS
                     LSGDPVVYSGNKIDSYLELLRKCAEQISYGLGYRNENEHLRKGN"
     gene            47665..48570
                     /locus_tag="POB34_000448"
     CDS             47665..48570
                     /locus_tag="POB34_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HDI5917455.1"
                     /translation="MKKFSGIIPPVSSTFHRDGTLDKKAMREVADFLINKGVDGLFYL
                     GTGGEFSQMNTAQRMALAEEAVTIVDGRVPVLIGVGSPSTDEAVKLAQHAQAYGADGI
                     VAINPYYWKVAPRNLDDYYQQIARSVTLPVILYNFPDLTGQDLTPETVTRLALQNENI
                     VGIKDTIDSVGHLRTMINTVKSVRPSFSVFCGYDDHLLNTMLLGGDGAITASANFAPE
                     LSVGIYRAWREGDLATAATLNKKLLQLPAIYALETPFVSLIKYSMQCVGLPVETYCLP
                     PILEASEEAKDKVHVLLTAQGILPV"
     gene            48581..50548
                     /gene="yjhG"
                     /locus_tag="POB34_000449"
     CDS             48581..50548
                     /gene="yjhG"
                     /locus_tag="POB34_000449"
                     /EC_number="4.2.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylonate dehydratase YjhG"
                     /protein_id="HDI5917456.1"
                     /translation="MSVRNIFADESHDIYTVRTHADGPDGELPLTAEMLINRPSGDLF
                     GMTMNAGMGWSPDELDRDGILLLSTLGGLRGADGKPVALALHQGHYELDIQIKAAAEV
                     IKANHALPYAVYVSDPCDGRTQGTTGMFDSLPYRNDASMVMRRLIRSLPDAKAVIGVA
                     SCDKGLPATMMALAAQHNIATVLVPGGATLPAKDGEDNGKVQTIGARFANGELSLQDA
                     RRAGCKACASSGGGCQFLGTAGTSQVVAEGLGLAIPHSALAPSGEPVWREIARASARA
                     ALNLSQKGITTREILTDKAIENAMTVHAAFGGSTNLLLHIPAIAHQAGCHIPTVDDWI
                     RINKRVPRLVSVLPNGPVYHPTVNAFMAGGVPEVMLHLRSLGLLHEDVMTVTGSTLKE
                     NLDWWEHSERRQRFKQLLLDQEQINADEVIMSPQQAKARGLTSTITFPVGNIAPEGSV
                     IKSTAIDPSMIDEQGIYYHKGVAKVYLSEKSAIYDIKHDKIKAGDILVIIGVGPSGTG
                     MEETYQVTSALKHLSYGKHVSLITDARFSGVSTGACIGHVGPEALAGGPIGKLRTGDV
                     IEIKIDCRELHGEVNFLGTRSDEQLPSQEEATAILNARPSHQDLLPDPELPDDTQLWA
                     MLQAVSGGTWTGCIYDVNKIGAALRDFMNKN"
     gene            50655..52004
                     /locus_tag="POB34_000450"
     CDS             50655..52004
                     /locus_tag="POB34_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDI5917457.1"
                     /translation="MPLIIVVAGIALLLLLTIKIKLNTFVSLIIVSIAVAIASGMDLS
                     KVVTSVESGLGGTLGHIGLIFGFGVMLGRLLADAGGAQRIALTMLNYFGKNKLDWAVV
                     CSAFIVGIALFFEVGLILLVPILFAIAREAKISPMFMCVPMLSGLLVAHGFLPPHPGP
                     TVIAREYGADVGLVLIYGIIVGIPTFILCGPVLNKFCQRIIPDAFKKEGNIASLGATR
                     RFSESEMPGFGISFLTAMLPVILMAVVTIIQMTHAKSAADSGLFYNVILFLGNSTIAM
                     LISLLFAIYTMGLGRGKTIPDLMDSCGKAIAGIAGLLLIIGGGGAFKQVLIDSGVGQY
                     ISTLVSGMDINPILMAWGVAAFLRICLGSATVAAISTAGLVIPLLAVHPNTNLALITL
                     ATGAGSCICSHVNDASFWMIKDFFGLTTKETLLSWTLMSTLLSISGLIFILLASLVL"
     gene            52351..53337
                     /locus_tag="POB34_000451"
     CDS             52351..53337
                     /locus_tag="POB34_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDI5917458.1"
                     /translation="MDRDQTNSSLFNDDPVLHATWLYYQEGKSQTEVAAIMGVSRVTV
                     VKYLQTARENGLVHINLDVNVFGSIDAALQIRDKFNLQRVIIVPDGEHAGKRDDTKLM
                     RTRLSRAGGMYLNQVIENGDVLGVAWGRTIHQMSKTMTPKSCKNVTVIQMLGSMPSQP
                     DLTIIESSSQIAYKLSGRVASLHVPAVVSSARLAMELQAEPIIRSNFDVLTRCTKAFF
                     VVGNALDENPLIRVGVLNKKEMQTYRDLGAVGVICGRFYDKEGMPVVADVDQRILGIS
                     LAQLRQIERKIFLAGGERGYDATLGALLGGYVTDLIVDEGTAEFLLACELPH"
     gene            complement(53378..54382)
                     /locus_tag="POB34_000452"
     CDS             complement(53378..54382)
                     /locus_tag="POB34_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HDI5917459.1"
                     /translation="MFIVESYAVAIIMCFITMICWGSWANTTKLVSNKKWEFPLFYWD
                     YSIGLLLCSLLFAFTLGSMGEAGRSFIPDIQQASSSSLMSAILAGIIFNISNILLVAS
                     INLAGMAVAFPVGVGLALALGVITTYIGNPQGDPLILFLGVACVVIAIIFTAIAYGRV
                     TQEADKSRRNKGLITAILAGIIMGWFFRFLADSMSDNFSQPASGLMTPYSALVLFAVG
                     LFLSNFVLNTLVMKKPISGEPVNGKMYFSGSLRDHVCGWLGGMIWCVGLAFSLIASGQ
                     AGYAISYGLGQGATMIAVIWGVFIWREFASAPAGTNKLLLTMFISYIVGIVLIIAANQ
                     "
     gene            complement(54486..55127)
                     /locus_tag="POB34_000453"
     CDS             complement(54486..55127)
                     /locus_tag="POB34_000453"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDI5917460.1"
                     /translation="MERIKLAEKIISTCREMNASGLNQGTSGNVSARYTGGMLITPSG
                     IAYSKMTPDMIVFVDDKGIPEAGKIPSSEWLFHLACYKARPELNAVIHTHAVNSTAVA
                     IHNHSIPAIHYMVAVSGTDHIPCIPYYTFGSPELADGVSKGIRESKSLLMQHHGMLAM
                     DVTLEKTLWLAGETETLADLYIKCGGLHHDVPVLSEAEMTIVLEKFKTYGLKA"
     gene            complement(55158..56273)
                     /gene="mtnA"
                     /locus_tag="POB34_000454"
     CDS             complement(55158..56273)
                     /gene="mtnA"
                     /locus_tag="POB34_000454"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HDI5917461.1"
                     /translation="MNIKGKHYRTVWVSGDGKAVEIIDQTKLPFKFEVVALTSAEMAA
                     TAIQEMWVRGAPLIGVVAAYGIALGMNHDASDMGLQRYYDLLIKTRPTAINLKWALDR
                     MIDTLKDLCVSERKDVAWALAAEIAEEDVALCEQIGLHGTEVIREIAQKKPAGSVVNI
                     LTHCNAGWLATVDWGTALSPIYKAHENGIPVHVWVDETRPRNQGGLTAFELGSHGIPH
                     TLIADNAGGHLMQHGDVDLCIVGTDRTTARGDVCNKIGTYLKALAAHDNHVPFYVALP
                     SPTIDWTIEDGKSIPIEQRDGKEQSHVYGINPQGELSWVNTAPEGTRCGNYAFDVTPA
                     RYITGFITERGVCAASKSALADMFADLKSKALQGEQH"
     gene            complement(56283..57542)
                     /locus_tag="POB34_000455"
     CDS             complement(56283..57542)
                     /locus_tag="POB34_000455"
                     /EC_number="2.7.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose kinase"
                     /protein_id="HDI5917462.1"
                     /translation="MTDSIPSGYKPLTCDTLPGYLSSRLTPSCEPGGLPEEWKVSEVG
                     DGNLNMVFIVGGTHKTIIVKQALPWLRAGGEGWPLSLSRAGFEYNVLCQEAKYAGHTL
                     IPQVYFYDPEMALFAMEYLTPHVILRKELINGKKFPKLAEDIGRFLAQTLFNTSDIGM
                     SAEQKKALTAEFALNHELCKITEDLIFTEPYYNAERNNWTSPELDDAVHKAWADVEMI
                     QVAMRYKYKFMTEAQALLHGDLHSGSIMVTDTDTKVIDPEFGFMGPMAFDIGNYIGNL
                     LLAYFSRPGWDANEQRRADYQEWLLQQIVQTWSVFTREFRQLWDNKTQGDAWPTEMYQ
                     QNRAALEDAQDQFFATLLEDSLVNAGMEMNRRIIGFAGVAELKQIENTELRAGCERRA
                     LTMARDLIVNARQFKNMDSVIQSAKVK"
     gene            58342..58596
                     /locus_tag="POB34_000456"
     CDS             58342..58596
                     /locus_tag="POB34_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDI5917463.1"
                     /translation="MKNGIDVSVIIPAYNAAETSVSLVTKILSEPHLAIELIIVDDGS
                     TDETGNVLRQIHDDRLILIEQANQGVYAARNAALALHQGE"
     gene            59700..61775
                     /locus_tag="POB34_000457"
     CDS             59700..61775
                     /locus_tag="POB34_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI5917464.1"
                     /translation="MDLVDSVEAGKLTISELIDALAKDKNYSASKWDQRYREFTTLLQ
                     QTSTFSEPETDGLVKRLWYERDNGIASIRQGVPSLAEYQQSLPLLRELTERIRQQPDE
                     ETYQYVGNALQQAKENGLLKRMYRSLRNRVFAAFSPENYTSTVDENAFSKAAEFLNQH
                     FHLGLALTGNWLQKNYELKQAIHAQSPDTDPYYVNMAIWHLYELLRERDNEQKQEKIA
                     STTFITSNEPIENKIILHSPTNVIFFGPPGTGKTFRLQQKMKEYTSHAVPADRDAWLD
                     SRLESLNWMQVITLVLLDLGKRAKVRQIIEHMWFQRKALLNGRNGNLSNTAWAALQSY
                     TVPESLTVDYKNRREPAVFNKTDNSEWLLVDSQLEQVEDLVELYAELKRGPKSAEAIQ
                     RFAVVTFHQSYGYEEFIEGIRARSDESGNISYPIEPGIFMRLSQRANADPGHRYAIFI
                     DEINRGNISKIFGELISLIEVDKRAGMPNAMSLQLAYSGDHFSVPANVDIIGAMNTAD
                     RSLALMDTALRRRFDFVEMMPDLSLLSGAKVKGIELESLLEKLNSRIEALYDREHTLG
                     HAFFMPVKNALDAGDEEAAFKQLKIAFQKKIIPLLQEYFFDDWNKIRLVLADNQKQDD
                     NLQFVIEKTDDLDTLFGNNHGLRHHDQQSTAYELKDFDQEIWNIPQAYRSIYQPQQTP
                     LDEQAVNHG"
     gene            61768..63054
                     /locus_tag="POB34_000458"
     CDS             61768..63054
                     /locus_tag="POB34_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HDI5917465.1"
                     /translation="MGEVISVFEYDLLGSDKAASVGAKLVPPLVFNYLEALSLASNQG
                     SQFLKLTSRSGFKLLQVQNYAGMLSTPHGFQLEILPKVGKNLTAANARQTLLTMLSHL
                     PGFRHIETQQATLQAQRMPLLEIFIHQFLHSVSQLLKQGLRSDYVSEQGNLAFMKGKL
                     MLSAQLRHNAVNRHKFCVDYDDYMPDCAANRLLHSALDKLLSLKLSSENQRWLYELRF
                     AFDGIPLSRDIERDINNLRLERGMAHYNEPMAWAQLILRGMSPSALQGNTKAISLLFP
                     MEAVFESFVAQTLPYELPSHLKVIPQAATYSLVKHGSNDCFKLRPDLLIQSRRPVQTK
                     LVMDTKWKLINSNSQKTTQYGLAQADFYQMFAYGQKYLNGIGEMYLIYPAHDQFNQPI
                     QQHFAFSKTLKLWVVPYQITANRGERMMWVKDVSSI"
     gene            63164..64903
                     /locus_tag="POB34_000459"
     CDS             63164..64903
                     /locus_tag="POB34_000459"
                     /EC_number="3.1.21.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Alw26I/Eco31I/Esp3I family type II restriction
                     endonuclease"
                     /protein_id="HDI5917466.1"
                     /translation="MKMGKIAVTPNNDKAASHSKDENLATNTIKKNVKPKYGDAAFLE
                     YTRLIVNHPNYFGMPDPFGEKGEIQWEAPSNRASGKFKHTHQRRYEWWKNKARSIGID
                     PDTEKAWISKTAKLIHPLGVKPCKKCGKEMELSYSYPNEHFFSRVRKLNYIDETFELS
                     QNEHIVDLLTRLDDRFGERIYLDLPHLFSTKSITIPDISSNLEAWIEYLKEQYIPQES
                     RMLSPGAMANPPDRFDGFHSFNRCCRSIADKGRTKENLKSYVTDRRVFEYWVDGDWVA
                     ADRLMGQVRTNNIFINEECLNAGNGGLHPTPCQADHIGPISLGFSHRPQFQLLCKSCN
                     SAKNNRMYLSDIISLLEAENEGHTVISWFAEEVWNRLKHSVDDSEKALRLSKILRDNR
                     HTYMNLLKKIMDEGYYTFLASLLHLEVANYNPIFEGLCISNHLTHYKSLKKIKRESKY
                     AAVQKTRRIRIAFTSLNDYHRKENRNAFIVSNELSEKFFSEAMDNLKSLSEITSCLDE
                     KISGIISENSDSKNEFRTIITDLREIVTNNKEKFNLILKYLISGMSEIGKELESYWEN
                     DRYVRSIPEEFIE"
     gene            complement(64916..66106)
                     /locus_tag="POB34_000460"
     CDS             complement(64916..66106)
                     /locus_tag="POB34_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDI5917467.1"
                     /translation="MTKSETFMIPNHKAAKLSELDMMIVNSVPPGGNWKNIPLDVPSK
                     RIEQIRDSYAQGKGSRSTYYGRLLPDMPAYTINTYFNRPGNGCHIHYEQDRVLSQREA
                     ARLQSFPDDFIFFGGQTAINTQIGNAVPPFLAFLIAKEIEKAIGNTGYYIDLFSGAGG
                     LGLGFKWAGWTPLLANDIEEKYLQTYSNNVHKEVLCGSISDNETFSKIADKISGFKKL
                     YFDKQLWILGGPPCQGFSTAGNARTMDDPRNSLFMHYKSLLNEIKPNGFIFENVAGLL
                     NMEKGKVFERVKEEFSSTMKTMNGWILNSEHYAIPQRRKRVILVGSNDPLFSIEPPQK
                     LTEDKESWVSVKDALSDLPPLQHGEDGSGKYYIHHPENDYQLFMRGNITPSEYYERNI
                     KPSL"
     gene            complement(66099..67739)
                     /locus_tag="POB34_000461"
     CDS             complement(66099..67739)
                     /locus_tag="POB34_000461"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Alw26I/Eco31I/Esp3I family type II restriction
                     adenine-specific DNA-methyltransferase"
                     /protein_id="HDI5917468.1"
                     /translation="MEEIFYMKHIHLINSLSLDETTKFTKKATGKYYTDPKIALLMIE
                     KLLPLINSCDKKSYNVADPFSGDGRLITLLIKQWMINGFPDVEWNVYLFDIENTGLTY
                     AKNALSELKLAGANINITIKNSDVFYEFKKYVDYFDCVITNPPWENIKPDSRELDFFE
                     PSMKSMYIDSLREFDDYLSRVLPYSQPKRKFAGWGTNLSRVGAELSLEICNKNGLVAI
                     VMPASFFADEQSYILREKFFNSGRIDCINYYPAEAKLFGGADVSSCSFIFNKGESLND
                     NIQLSVYDKNLNIKSLGFFDLSSIDSQYLSIPVSQGVHAVHLLRKLQEGYPTWGSLEK
                     NGEIWAGREIDETGSSDWTQKSGGGLLFIKGKMIGRYNFHNEKSLRITKKIDKVLSNS
                     NFVRIAWRDISRPSQKRRMIATIIPPNSLAGNSLGVVYYKSGSQDSLFSLLGIINSLC
                     FEFQLRSFLATGHVSLSALRKTAIPSEKILLQHSELKQLVISCIEGCCDAELKIEAYV
                     AKNIYKLDLNEFNKLLSSFDKIELAEKESLLRIFQHYD"
     gene            complement(68135..68881)
                     /gene="istB"
                     /locus_tag="POB34_000462"
     CDS             complement(68135..68881)
                     /gene="istB"
                     /locus_tag="POB34_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="HDI5917469.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTA
                     EELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNIL
                     LTGATGSGKTFLACALGHNACRQGYKVYYYRLKALMEQCYQGHADGRYSKLLTRLNNS
                     DLLLLDDWGLEPLSSEQRSDLLEIVDLMYQRGSIIVVSQLPVENWYKMIGDSTHADAI
                     LDRLVHGSIKIELKGESMRKIQSPLTEGDQ"
     gene            complement(68896..69264)
                     /locus_tag="POB34_000463"
                     /pseudo
     CDS             complement(68896..69264)
                     /locus_tag="POB34_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            69421..69570
                     /locus_tag="POB34_000464"
     CDS             69421..69570
                     /locus_tag="POB34_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HDI5917470.1"
                     /translation="MEKVISHTRYKLTPAELEAFNSAVDNRLAELTMNKLYDRVPASV
                     WKYVT"
     gene            70300..70869
                     /locus_tag="POB34_000465"
     CDS             70300..70869
                     /locus_tag="POB34_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDI5917471.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLERDDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            71129..71530
                     /locus_tag="POB34_000466"
     CDS             71129..71530
                     /locus_tag="POB34_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917472.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            71518..71952
                     /locus_tag="POB34_000467"
     CDS             71518..71952
                     /locus_tag="POB34_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HDI5917473.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            72307..72687
                     /locus_tag="POB34_000468"
     CDS             72307..72687
                     /locus_tag="POB34_000468"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDI5917474.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            72684..73031
                     /gene="tnpB"
                     /locus_tag="POB34_000469"
     CDS             72684..73031
                     /gene="tnpB"
                     /locus_tag="POB34_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDI5917475.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            73081..74409
                     /locus_tag="POB34_000470"
     CDS             73081..74409
                     /locus_tag="POB34_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDI5917476.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVPGVIAD"
     gene            complement(74705..76063)
                     /locus_tag="POB34_000471"
     CDS             complement(74705..76063)
                     /locus_tag="POB34_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HDI5917477.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            76345..76476
                     /locus_tag="POB34_000472"
                     /pseudo
     CDS             76345..76476
                     /locus_tag="POB34_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(76814..77071)
                     /locus_tag="POB34_000473"
     CDS             complement(76814..77071)
                     /locus_tag="POB34_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HDI5917478.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT        19865 a        18790 c        18354 g        20320 t
ORIGIN      
        1 ggcattcatt ccatcagcgt agccttgcag aatggacata tcctctgggg aaagggcagc
       61 aatttgcgcc cggatagcat ccggccagta gttacgacgg atatctttat caaatttcac
      121 aaaatctttg ccaagcactt ccgcgacagt cccttgagta ctgcgacgtg ccatttccat
      181 ctgaaaaagg cgatcttgtg ctactacata gccatagcca taaaataggt gccatgtatc
      241 attggcataa atatgcggca tgccgtattc atcgcgaaca atctttatct cacttgacga
      301 ctgctcagcc agtgcaggta agctccaata atacatcagg gaagcagtaa cacagttcac
      361 gatcatacga tttctatttt tcattgtatc ctctggcagg tgtataatta gcgaattgat
      421 actagcaacg aagcttcggt tatctatttg cagagaattg tttcattatg aactgtgagc
      481 gcgataacta atttttgata caaaaatttc attaatgtga tctaaaacga atcgctttat
      541 tcaccgctga tgattttgtt ctattccttt ttgtgacaga tcccccggac gcatagatga
      601 tgacccccag agagacagct caggtcatta tctgtatgta aatcagaaaa atatgttgta
      661 aacgactttt atggttggat tttgtagcaa ttttgaatgc agacaggcag catttgccaa
      721 cacgccgccc atagttcact ccacatcctc cctgcgtaac ccgatatccc tcaaccgttc
      781 atcactcatc tgctgtaaca cccgtcgggc ctgcatttgc cgccgccaac gcctcaccgc
      841 ctgccagagt tgcacaagtc tgataaacgg cgttttggct ttgttttcgt gaaattccat
      901 ctgctgcctc ctcatctttc agaggcttca tcatccgtct tctggcagtt cacaatacag
      961 atgcaaatat tagttttatt taacatacag acaggttaaa acagcatctg catccggttt
     1021 tttagcctca tctgtaccgg tttttctttc tgtatgggcc aaataaggaa tacagcatga
     1081 cgcgttatca acatctggcg actctacttg ccgagcggat tgagcaaggg ctgtatcgtc
     1141 acggggagaa attgccgtcg gtgcgcagct taagtcagga gcacggcatc agcatcagca
     1201 ccgtgcagca ggcgtaccag acgctggaga cgatgaagtt catcactccg cagccgcgtt
     1261 cgggttattt tgtcgcacaa cgtaaagccc agccgcctgt accgccgatg acgcgtccgg
     1321 tgcagcgccc ggtggaaatt acccagtggg atcaggtgct ggatatgctg gaggcgcata
     1381 gcgacaattc cattgttccg ttaagcaaaa gcacgccgga tgtcgaaacg cccagcctga
     1441 aaccactctg gcgtgagcta agccgggtgg tgcagcataa tctacaaacc gtgctcggtt
     1501 atgacttgtt agccggtcag cgagtgttgc gggagcagat tgcccgcctg atgctcgaca
     1561 gcggctcggt ggtcaccgcc gatgacatca tcatcaccag cggctgccat aactcgatgt
     1621 cgctggcgtt aatggcggtg tgtaaaccgg gcgatattgt cgcggtcgaa tctccctgtt
     1681 attacggttc gatgcagatg ctgcgaggca tgggcgtgaa agtgattgaa atcccaaccg
     1741 atccagaaac tggcatcagc gttgaagcac tggaactggc gctggaacag tggccgatta
     1801 aaggcatcat tctggtgcca aactgtaata atccgctggg atttattatg ccggacgcac
     1861 gcaaacgggc cgttctctct ctcgctcagc gtcatgatat tgtgattttt gaagatgatg
     1921 tctacggcga actggcaacg gagtatccgc gcccgcggac catccattca tgggatatcg
     1981 acgggcgagt gctgttgtgc agctcgttca gtaaaagtat tgctccaggc ctgcgcgtgg
     2041 gttgggtcgc accggggcgt tatcacgata aactgatgca tatgaaatac gccatcagca
     2101 gctttaatgt gccgtccacg caaatggcgg cggcaacgtt tgtgttggaa ggccactatc
     2161 atcgccatat ccggcggatg cggcagactt atcagcgcaa tctggcgctt tatacctgct
     2221 ggatacggga atattttccc tgcgaaatct gtattacgcg cccgaaaggc ggatttttac
     2281 tgtggatcga attgcctgaa caggtcgata tggtctgcgt cgcgcggcag ctgtaccgca
     2341 tgaaaatcca ggtggcggca ggctcgattt tctcggcttc cggcaaatac cgtaattgtc
     2401 tgcgcatcaa ctgcgctttg ccgctcagcg aaacctatcg cgaagcacta aagcaaattg
     2461 gcgatgccgt gtatcgggca atggaataaa ttcgaattag tgccgaggcc cctgatttac
     2521 tctcacgaat aacctgtgac tcgttttatc tttatagcga catcgccacg ttccggcatg
     2581 gcttaggcaa agtgtgcttc gttcatgcca gatgccatgt gaacgcctta ccaggtctac
     2641 aaaatattaa aagttcaata tatttaatgg attatgtaga cctgataagc gtagcgcatc
     2701 aggctgcttt gcgtttgtca tcagcctctg attagtactc gatccatggt tcgcgcttgc
     2761 tttcaggcaa gggctgatct aatgcctgca tttgctctgc cgaaagtccg gtatatcgtt
     2821 gtatccattc aggatcggcc ccattctgca acaacaatcg gaggaggctc cgttcccctt
     2881 ctactttccc ttctttcctc cccctctccc ttcccaacag ccatccatca ttataaattc
     2941 gctctgcaat cgtcattatt cgctccctgt gttgtggcat tcgacgggta agttcgctga
     3001 taaacttctt aaaacgcgct tcatcgccag tcagtaaaat gtaatttaac agcgcagtta
     3061 tctggctgtc attagcacac tctgtaacca gtaatattac cagttgatcg ataagcccca
     3121 tcagatcgcg ctggcgaata tgcttttgga tcaactccaa cagggcgact ctgcggtgct
     3181 gcacaatctc gtcgtctggc acgacagtga catccaccag cgggaacgct gcggtataaa
     3241 gcttccgtgc ggtggtcggg tcagcaaatt cgtccagcca gcacagggac cagggataag
     3301 gactacggct accgtgataa aacagcatcg ggatgaccag cggtagcggc cggcgtttat
     3361 catgctcgat atggcgctgc atcaccgcca tggaatatcg catcaggcga aaggccatat
     3421 ggatatcctc gcggctctga tgttcaatca ctacgtaaat ataaccatca ccttcacggg
     3481 tctttaccga ccacagaata tcggagtgta gcgcccgcaa tttttcatcg acgaagctgg
     3541 cggattccag ttttaagctg tcgagatcgc acagttcacg taaatctttg ggtaagtgga
     3601 tctccataaa atcccgcgcg gtgtcagggt gcgtgagaaa ggatttaaat aatgcgtcat
     3661 gcggcgtgct ggttgtgaag tttgtcatgg cgatccgtca cctgtagata agatggcccg
     3721 actgtagcga ctaacaaaac cattctcacc cggcaatttc tcgttccccg aggcgctttc
     3781 caggctattt ttgttcctct gcaaacgata aatttttccg gaaacaggct cgcgttttac
     3841 atttcctgta cccggcagat attccccttt ccaccgcact cttttgatta accgcaaatt
     3901 ttaattacac ttaaggtgta tattttctat gcaacccatc aattcaagag gggtaatgtg
     3961 ctgatgacta tttgtaatcg ttatacattc tgacctggag tcagaaggta tttctctgtc
     4021 tgtgtgttca caggcagtgt ggttgattac atgaaattca gtacatttgt ccttctcgcc
     4081 tgcctttagt cattaccgac ggtattgaat ttcgttttcc ccattggggt tctccggaca
     4141 aggagttgtt tgttatgcca cgtttttttg cccgccatgc cgccacgctg tttttcccga
     4201 tggcgttgat tttgtatgac tttgctgcgt atctgtcgac ggacctgatc cagccaggga
     4261 tcattaatgt ggtccgtgat tttaatgccg atgtcagtct ggcccctgct gccgtcagtc
     4321 tctatcttgc tggcggtatg gcgttacagt ggctgctggg gccgctttcc gacagaattg
     4381 gccgcaggcc ggtgctgatt accggggcgt taatttttac ccttgcctgc gccgcgacaa
     4441 tgttcaccac gtcaatgaca caatttctta tcgcgcgtgc aattcagggc acaagtatct
     4501 gttttattgc caccgttggt tatgtcacgg tgcaggaggc gttcggacag acaaaaggga
     4561 tcaagttgat ggcgattatc acctccatcg tactgattgc gccgattatt ggcccgcttt
     4621 ccggcgcagc tctgatgcac tttgtgcact ggaaagtcct ttttgccatc attgcggtta
     4681 tgggttttat ctcatttgtt ggcttactgc tggcgatgcc agagacggtg aagcgcggcg
     4741 cggttccatt tagcgccaaa agcgtcttgc gcgattttcg taatgtcttt tgcaatcggc
     4801 tgttcctctt tggcgcagca accatctctt taagctatat cccaatgatg agttgggtgg
     4861 ctgtctcgcc ggtgatcctg atcgacgcag gcggcttaac gacctcgcag ttcgcctgga
     4921 cacaggttcc ggtgttcggc gcggtgattg tcgcgaatgc catcgtggcg cgtttcgtta
     4981 aagatccgac cgaaccgcgc tttatctggc gtgccgtacc ccttcaactg gttggcctcg
     5041 cactgttgat tatcggcaat ctgctgtcac cgcacgtctg gctgtggtcg gtgctgggca
     5101 ccagtctgta tgctttcggg attggtttga ttttcccgac cttattccgc tttacgctgt
     5161 tttccaataa cttaccgaaa gggaccgtct ccgcatcgct gaatatggtg atcctgatgg
     5221 tgatgtctgt ctcggtcgaa atcggccgct ggctgtggtt taacggcggt cgcttgccgt
     5281 ttcatctgtt agccgtcgtg gcgggcgtta tcgtcgtttt caccctggca ggattgctca
     5341 atcgcgtgcg ccaacatcag gctgccgagc tggcggagga gcggtgattt ttgcgcgatc
     5401 ctgccgtcag gctctattct taacgttatg aataaactca ttgaactcag acgcgccaaa
     5461 atgttggcgc tctctttact gcttatcgcc gctgctacct ttgtcgttac gctgtttttg
     5521 ccgcccaatt tttgggtgag cggcgtgaag gcgattgctg aagcggcgat ggtcggcgcg
     5581 ctggcggact ggtttgcggt ggtggcgctg tttcgccgcg tgccgattcc gatcatttct
     5641 cgtcatacgg cgattatccc gcgtaataaa gaccggattg gcgaaaatct cggtcagttc
     5701 gtgcaggaaa aatttctcga tacccagtcg ctggtggcat tgattcgacg tcacgaaccg
     5761 gcgttgttga ttggcaactg gtttagtcag ccggagaacg cccgccgcgt tggtcagcat
     5821 ctgttgcaga tcatgagcgg ttttcttgaa ctgaccgatg atgcgcgtat tcagcgcctg
     5881 cttaagcgcg cggtccatcg ggcgattgat aaggtcgatc tttccggaac cagtgcgttg
     5941 atgctggaga gtatgaccaa aaacgatcgt catcaggtgc tgctcgatac gctgatcgca
     6001 cagttgatcg cccttctcca gcgcgataaa tcgcgcaagt ttattgccca gcaaattgtt
     6061 cgctggctgg agagtgagca tccactgaaa gccaaaattc tccccaccga atggttgggc
     6121 gaacatagcg cggagttggt ttctgacgcg gtgaattctt tgcttgatga tatcagccgt
     6181 gatcgtgcgc atcagatccg tcatgcgttt gatcgcgcca cttttgccct gatcgacaag
     6241 ttgaaaaacg atccggaaat ggcagcgcga gccgatgccg taaaaagtta tctgaaagaa
     6301 gatgaagctt ttaaccgcta tctcagtgaa ttgtgggggg atttacggga gtggctgaaa
     6361 gcggatatca acagtgaaga ttctcgtgtg aaagaacgta tcgcgcgggc gggtcaatgg
     6421 tttggcgaaa cgttaattgc cgatgatgcc ttgcgggcgt cgttaaatgg tcacctggaa
     6481 caagccgcgc accgcgtcgc gcctgagttt tccgcattcc tgacgcgcca catcagcgat
     6541 acagtaaaaa gctgggatgc tcgggatatg tcgcggcaaa tcgagttaaa tatcggcaaa
     6601 gatctgcagt ttatccgtgt caacggtacg ctggttggcg gttgtattgg gctaatttta
     6661 tatttgttgt cgcagctccc ggccttgttc cccctcagca atttttagaa atccatgaaa
     6721 aactaagtgg ctaaccgcga gagagatcaa ataaaccaca ctaaaagaat ggtaataatg
     6781 tggcctcttt tcattatctc ccgtggtacg gggaaggaaa atcatgtcac ttatcaccga
     6841 tctacccgcc attttcgatc agttctctga agctcgccag aaaggctttc tcaccgtcat
     6901 ggatctcaag gagcgcggca ttccgctggt tggcacttac tgcaccttta tgccgcaaga
     6961 gatcccgatg gcagccggtg cggttgtggt ttcgctctgt tctacctctg atgaaaccat
     7021 tgaagaagcg gagaaagatc tgccgcgcaa cctctgcccg ctgattaaaa gcagctacgg
     7081 cttcggcaaa accgataaat gcccctactt ctacttttcg gatctggtgg tcggtgaaac
     7141 cacctgcgac ggcaaaaaga aaatgtatga atacatggcg gagtttaagc ccgttcatgt
     7201 gatgcagttg ccgaacagcg ttaaggacga tgcctcgcgt gcgttatgga aagccgagat
     7261 gctgcgcttg caaaaaacgg tagaagaacg ttttgggcac gagattagcg aagatgctct
     7321 gcgcgatgcc attgcgctga aaaaccgcga acgtcgcgca ctggccaatt tttatcatct
     7381 tgggcagtta aatcctccgg cgcttagcgg cagcgacatt ctgaaagtgg tctacggcgc
     7441 aaccttccgg ttcgataaag aggcgttgat caatgaactg gatgcaatga ccgcccgcgt
     7501 tcgtcagcag tgggaagaag gccagcgact ggacccgcgt ccacgcattt taatcaccgg
     7561 ctgcccgatt ggcggcgcag cagaaaaagt ggtgcgcgcg attgaagaga atggcggctg
     7621 ggttgtcggt tatgaaaact gcaccggggc gaaagcgacc gagcaatgcg tggcagaaac
     7681 gggcgatgtc tacgacgcgc tggcggataa atatctggcg attggctgct cctgtgtttc
     7741 gccgaacgat cagcgcctgc aaatgctcag ccagatggtg gaagaatatc aggtcgatgg
     7801 cgtagttgat gtgattttgc aggcgtgcca tacctacgcg gtggaatcgc tggcgattaa
     7861 acgtcatgtg cgtcagcagc acaacattcc ttatatcgct attgaaacag actactccac
     7921 ctcagatgtc gggcagctca gtacccgtgt cgcggccttt attgagatgc tgtaaggagt
     7981 ggcagtgaca tattcgattg gcattgattc tggttctacc gccaccaaag ggatcttact
     8041 ggcagacggc gtgattacgc gccgtttcct cgttccaaca ccctttcgtc cggcaacagc
     8101 aattactgaa gcctgggaaa ctctgcgcga agggttagag acaacgccgt ttctgacgct
     8161 caccggctat ggacggcaac tggtggattt tgccgataaa caagtgacgg aaatctcctg
     8221 tcacgggctg ggcgcacggt ttcttgcgcc agcaacgcgc gcggtaatcg acatcggtgg
     8281 tcaggacagc aaagtgattc agcttgatga tgacggtaac ctgtgcgatt tcctgatgaa
     8341 tgacaaatgc gcggcgggca ccgggcgttt cctggaggtg atctcgcgca cgcttggcac
     8401 cagcgtcgag caactcgaca gcattaccga aaatgtcacg ccgcatgcca tcacgagtat
     8461 gtgcacagtg tttgccgaat cagaagtgat cagcctgcgc tcagcgggcg tcgcgccaga
     8521 agcgattctc gccggagtga ttaacgcgat ggcgcggagg agtgccaatt tcattgctcg
     8581 tctctcctgt gaagcgccga ttctgtttac tggtggcgtt agtcattgcc agacgtttgc
     8641 ccggatgctg gaatctcatc tgggaatgcc ggtaaatacc catcctgatg cgcaatttgc
     8701 tggcgcaatt ggcgcggcgg taattggtca acgagtgagg acacgccgat gaaagagttt
     8761 ttatttttat ttcactccac agtcggcgtc atacaaaccc gcaaagcgtt gcaggcagcg
     8821 ggcatgactt ttcgcgtcag cgatattccc cgtgatttac gcggcggctg cgggttatgc
     8881 atttggctga cctgtccccc cggcgaggaa atacaatggg tgatccctgg gctgactgag
     8941 tcaatttatt gtcagcagga tggtgtctgg cgctgcattg cacattaccg ggtttctccg
     9001 cgttaaggat tcattaaggt cgctacgtat acttctcgat atcttattga tttgccttaa
     9061 agggattacc gtgacaaaaa gtatttcgtt aagtaaacgc atctccgtta ttgtgattct
     9121 ttttgccatt gtggcagtgt gtactttctt cgtgcaatct tgcgcccgta aaagcaatca
     9181 tgcagcatca tttcagaatt accacgccac cattgacggc aaagaaattg caggcataac
     9241 gaataatatc tcttcgctga cctggtcggc acaaagcaat actcttttta gcactatcaa
     9301 caaacctgcc gccattgttg aaatgaccac caatggtgat tttatccgca caattcctct
     9361 ggattttgtt aaggatctcg agacgatcga atacattggc gataatcagt ttgtcattag
     9421 cgatgagcgc gattacgcca tttatgtgat ttcactgacc ccaaactcgg aagtaaaaat
     9481 cctcaaaaaa ataaaaatcc cgctgcaaga ctccccaacc aattgcggtt ttgaagggtt
     9541 ggcttattct cgccaggatc acactttctg gttttttaaa gagaaaaatc cgattgaggt
     9601 ctacaaagtt aacgggctgt taagtagcaa tgaattacac atcagcaaag acgaggcact
     9661 acaacggcaa tttactttag atgatgtgtc cggcgcagaa tttaaccagc aaaaaaatac
     9721 gcttctggtg ctgtctcatg aatctcgcgc actccaggaa gtaaccctgg ttggcgaggt
     9781 tatcggtgaa atatcactaa ccaaaggtag tcgaggtttg tcgcataaca tcaaacaggc
     9841 cgaagggatt gcgatggatg cctctggtaa tatttatatc gttagcgaac caaatcgttt
     9901 ttatcgcttt acccctaaat catcgcatta acaggctggc atatttcacc tttttgttat
     9961 taacttgtta acaacaaaaa tgacatacaa ggttaaacat gccttcgtcc tcgcatcccg
    10021 tagaacgctt ttctttcagc accgcgcttt tcgggatgct ggttctgacc ctaggtatgg
    10081 gattaggtcg cttcctttat acgcctatgt tgcccgtcat gatggcggaa ggggcgtttt
    10141 catttagcca gctctcgtgg attgccagcg gcaactatgc ggggtatctg gctggcagtc
    10201 tgctattttc gtttggcgca tttcaccagc cgtcgcgcct acgcccattt ctgttggttt
    10261 ctgccctggc gagcgggttg ttgatcctcg caatggcatg gttgccgcca tttattctgg
    10321 tgttattgat tcgcttcctg gcgggtgtcg ccagcgccgg tatgctgatt tttggttcga
    10381 cgctgattat gcagcacacg cgccatcctt ttgtgctggc agctttgttt tctggcgttg
    10441 gcattggcat tgcactgggc aacgaatatg ttctggcagg cctgcatttt gccttctcct
    10501 cgcaaacgtt atggcaaggc gccggggcgc tttctggcat gatgctgatt gcacttacgc
    10561 ttttaatgcc ctcgaaaaaa cacgccatcg caccaatgcc attggcaaaa acggagcaac
    10621 agataatgaa ctggtggtta ctggctattc tgtacggcct ggcgggattt ggttatatca
    10681 tcgtcgcgac tttcctgccg ctcatggcga aagatgcagg ctcaccattg ttaaccgccc
    10741 atctctggac gttagtcggc ttatcaattg tgccaggctg ctttggctgg ctatgggcag
    10801 caaaacgttg gggggctttg ccttgcctga cggcgaattt gctggtgcag gctatcagtg
    10861 tgctgcttac tctcgccagc gactctcctc tcttgcttat cattagtagt atcggttttg
    10921 gcggtacctt tatgggcacg acatctctgg tgatgactat cgcccgccaa ctgagcgttc
    10981 cgggaaatct caatctttta ggctttgtga cgctcattta cggcattgga caaatcctcg
    11041 gcccggcgct gaccagtatg ctcggcaatg gaacatcggc gcttgccggc gccactctct
    11101 gcggcgcggc ggcgttgttt attgctgcgc ttatcagcac agtacaatta tttaagctgc
    11161 aagtggtcac ttcatgagga gtgtttccct taccactcaa gaaactcaac agcaaccgtt
    11221 gatgtcagcc agaccccgtt ttccgcttgc cagaaaggga tgcctcgttt cgccatttcc
    11281 tgtgctttga cggttaagat aaccggcgat ccgtgtcttg ctcccacttt gcgtgccgtt
    11341 gcttcatcag cagagagatg tacgtaatga cgctcccctg caatcagccc ctgttttttt
    11401 atctcgtcaa ggaaacggct tgccgtaccg tggtagagaa actgtggcgg tgttttttcg
    11461 gcaaaggaaa tggcgacctg cgaagtcgaa tgcccctgaa ccgcgcggat gcacctacca
    11521 tcactggaat aactaaaacg ttttttatca ctggtagcga ccacggtatc cagcaatgca
    11581 cgggttaacc gcttgcccgc tttttgggca caaagtatga gcttatcgat atccgcccat
    11641 ccttcacggt ccagtacgat acctatcgcc tccggtttgt gtcggaggac aaaacttaaa
    11701 aacttgctcg tatccgccag ctctttttcg ttgtactttg ccatcgcgtt ttcgtccctg
    11761 gattttctgt ttttatttac tgatgtcatt gtcactgggt ttatgaattt tctgcaactc
    11821 aatcctgcgc agagcctttt ccacacagca taatttttcc gctatgcaaa aaacgcatag
    11881 ccagaaacat acttgcattt tccccatacc cacatggctg ataaggtttt tccgctccag
    11941 gaattttcct ggctcaagca acacacaaac acaaaaaaac aatggggatt gttatgacac
    12001 aacaagggga tgctgtagcc ggggagctgg cgacagaaaa agtgggcatt aaagggtatc
    12061 tggctttttt tctcaccata atcttttttt ccggtgtttt ttccggaaca gacagctggt
    12121 ggcgcgtttt tgatttttcc gttctgaacg gatcgtttgg gcaactgcct ggtgcgaacg
    12181 gagccaccac ctcatttcgc ggggcgggtg gcgcgggggc taaagatggc tttctctttg
    12241 cgctggagct ggcaccgtca gtcattctct ctctggggat tatttcaatt accgatggtc
    12301 ttggtggatt acgcgccgcg cagcagttaa tgacgcctgt gctaaaaccg ttgttgggca
    12361 ttccagggat ctgctcgctg gcgctgatcg ctaacctgca aaacactgat gccgccgccg
    12421 gtatgaccaa agagctggcg caggaagggg aaattaccga acgcgataaa gtgattttcg
    12481 ccgcatatca aaccagcggt agcgccatca tcaccaacta cttctcttcc ggcgtcgcgg
    12541 tgtttgcctt tctgggcacg tccgtaatcg tacctttagc cgtcatcctg gtgtttaaat
    12601 ttgtcggagc caacattttg cgcgtctggc ttaacttcga agaacgtcgc aacccaacgc
    12661 aaggagcgca agcatgacaa ctcaggtccg caaaaacgtc atggatatgt ttatcgatgg
    12721 cgcgcgccgt ggctttacta tcgccacgac caacctactg ccaaacgtgg tgatggcgtt
    12781 tgtcatcatt caggcgctga aaattaccgg cctgctcgac tgggtggggc atatctgtga
    12841 acccgtaatg gcgctgtggg gattacccgg tgaagcggca accgtccttt tagccgcatt
    12901 aatgagtatg ggcggcgcag tcggtgttgc cgccagtctg gcgaccgccg gcgcattaac
    12961 cggtcacgat gtcaccgttt tactgcccgc gatgtattta atgggcaacc cggtgcaaaa
    13021 tgtcggtcgc tgcctgggga cagccgaagt gaatgccaaa tattacccgc atatcattac
    13081 ggtctgcgtg attaacgcat tgctgtcgat ctgggttatg cagcttattg tttaacaagg
    13141 agttaccatg attgattata ccgcagccgg ttttaccctg ctgcagggag cgcatttgta
    13201 tgcgccggaa gatcggggaa tttgcgatgt cctcgtcgct aacggcaaaa ttatcgccgt
    13261 tgccagtaat atccctcctg acattgtacc ggactgcacg gttgtcgatc tcagcgggca
    13321 gatcctctgc ccaggtttta ttgatcaaca cgtccatttg attggcggtg gcggcgaagc
    13381 aggtcccacg acgcgcacgc cggaagtggc gttaagtcgt ctgacggaag caggcgtcac
    13441 gtcagtggtt ggtctgctgg gcaccgactc tatctctcgc cacccggaat ccctgctcgc
    13501 caagacccgt gcgctcaatg aagaaggcat cagcgcctgg atgctgaccg gcgcttatca
    13561 tgtcccttcc cgcactatta cgggttccgt ggaaaaagac gtggcgatta tcgatcgcgt
    13621 gattggcgtg aaatgcgcca tctctgatca ccgttctgcc gcaccggacg tttatcacct
    13681 ggctaatatg gcggcggaat cccgcgttgg cggtttgctc ggcggtaaac ctggcgtcac
    13741 cgtgttccac atgggcgaca gcaaaaaggc gttacagcct gtctatgacc tgctggaaaa
    13801 ctgcgatgtg ccgattagca agttgctgcc gacccacgtt aaccgcaacg ttccgttgtt
    13861 tgagcaggcg ctggagttcg cgcgcaaagg cggcaccatc gatatcacca gcagcattga
    13921 cgaaccagtc gcccctgccg aaggtattgc ccgcgccgtt caggcgggta ttccgctggc
    13981 acgcgtcacc ctcagctccg acggcaacgg tagccagccg ttcttcgatg acgaagggaa
    14041 tttaacccat atcggtgttg ccggttttga aacgttgctg gaaaccgtgc aggtgctggt
    14101 caaagattat gatttcagta tcagcgatgc cctgcgcccg ctcaccagta gcgtagccgg
    14161 tttccttaac ctgagcggga aaggcgaaat tctgccaggc aatgacgcag acttactggt
    14221 catgacgcca gaattacgca ttgagcaggt atacgctcgc ggcaagctga tggtcaaaga
    14281 cggcaaagcc tgcgtgaaag gaacgtttga aacggcataa acggttgtca gccgcccgaa
    14341 tggacgtaga atgcccgcaa gggcggctga cagagtaaaa cgtaatggat gactgtggtg
    14401 cggttttgca taatattgaa actaaatggc tttatgattt tctgaccctg gaaaaatgcc
    14461 gcaatttttc ccaggcggca gtcagtcgca acgtctcgca accggcattc agccgccgca
    14521 tccgtgcgct ggaacaggcg attggtgttg aattgtttaa ccgccaggtg acgccgctgc
    14581 aactctcgga acaaggtaaa atattccatt cgcagatccg ccatctgttg caacagttag
    14641 agagcaacct tgcagaactc cgtggtggca gcgattacgc gcaacgcaaa attaaaatag
    14701 ccgctgcaca ctctctttcc ctcgggctgt taccgtccat tatcagccag atgccgccgc
    14761 tctttacctg ggcaattgaa gctattgatg tcgatgaagc ggtcgataaa ctgcgtgaag
    14821 ggcaaagtga ctgtattttt tcctttcacg acgaagattt gctgggagcg ccgtttgacc
    14881 acattcgctt atttgaatct caattgttcc ccgtttgcgc cagtgacgaa cacagagaag
    14941 cactttttaa cctcgcgcag ccacactttc cgttactgaa ttacagccgc aactcctaca
    15001 tggggcgatt gattaatcgc accctgacgc gccacagtga gttaagtttc agcacctttt
    15061 ttgtctcttc gatgagcgag cttttaaagc aggttgccct cgacggctgt gggattgcct
    15121 ggctaccgga atacgccatc caacaagaaa ttcgcagcgg gcagctcgtt gtactgaatc
    15181 gggacgaact ggtgatcccg attcaggcct atgcataccg gatgaatacc cgcatgaatc
    15241 cagttgccga acgcttctgg cgtgaactgc gcgagctgga gattgtgctt agctgacatt
    15301 ctccagcgtc gcactgcgcc aaccgccatt atcccagacc agatcaaacg ttagcgattc
    15361 atcgtcgtga tacagcatta tctcaaggct gatcgccaga gtgtggcagt acgcttcttt
    15421 caaattaacc tgcaacgctg caatccgtgg ctcatgaaat aacagtgcag aacgcagctg
    15481 acggcaatac cagctctgga gatgtttgtc gctcgggtta tgcacttgcc actcgggcat
    15541 gcctgaaaca agtaatgacg gtaatgccga atgaggagaa ttaaaaagag tgaggagatc
    15601 gctgcatact gatttacgca gcggtgacgt tttattgcct gaaatttcag gtaaaacagc
    15661 ctgaagtgat tggcgcatca ttttgcgcac tcctgacgtt tagcaacata tgcggcttgc
    15721 cctgaacacc gggcaccact gtattgatgc tcattttcca tagccattcc cttttgttca
    15781 ttacatccat gtcagtctga gacaggcctg aatcctgtca gcaaagtata aaaccttact
    15841 tgcctatgaa tatctactga cttttcagga aaaaagtcat gatttgataa aaatcatcaa
    15901 actcaaaaaa aaaccgctag tatattctga atattccgaa tagttacgta gatgaaaata
    15961 cttgtcacca ccgctactta attccggtgg gaaattattt atttccgcat aatcagccat
    16021 gtattattta aaacaattag tttgtaaata atataaattg ataacatatc aaatacatca
    16081 aaaataccat tcacaggcat tattaatata gacatcaata tagcaacatc aaattccaat
    16141 tagtaataag atatgctttt aaattgcatg caaataattt taaatgagcg ttttttgata
    16201 ctttagagtg tttcctaccc tttaatatca ccgtgtagag ttgatatcac tacaacagaa
    16261 acatgcatgt catttgttta cttctctttt tactacagaa aaatttatct atttttctaa
    16321 acatcagtga aatactccta aaaggtaact attatgtcta tgccattaag caatgccctt
    16381 cagagtcaaa ttattacaga taatcacttc cttcatcacc caaaagtcga caatgagtta
    16441 acacgtaaat atgaacgagc tcgcctggat acggaaaata tctatcttct gcctcttgct
    16501 cgtggcaata accataatta cgatggaaaa agcgttgtcg aaattagaaa acttgatatt
    16561 agtaaagagt cctggccatt taattatgtg acagaagcat gcagagaatc cgatggtatt
    16621 acaacaacag gacgtatgct ctatcgaaac ttgaaaatca cctcagcttt agacgaaata
    16681 tatggtggta tatgtaaaaa ggcgcatgct gctacagaat tagcagaagg tttacggcta
    16741 aatttattta tgaagagccc tttcgatcct gttgaggact atacagttca tgaaataaca
    16801 ttaggccctg gttgcaatgt tccaggttac gccggaacca cgataggata tatttcaacc
    16861 cttccagttt ctcaggcaaa aagatggacc aatgagcagc caagaattga tatctatatt
    16921 gatcagataa taactgtctc aggggtagca aattcttccg gattcgccct tgccgcatta
    16981 cttaatgcta atattggaat gggaaatgac ccaataattg gtatagaagc ctatcccggt
    17041 accgcggaga tccatgcaaa aatgggatat aacgttatcc ccggtgatga agatgcgcca
    17101 ctcaaacgca tgactctgca accgtcatct cttccagaac tgtttgaatt aaaaaatggt
    17161 gaatggaatt atattggtaa atagccactg agaagaatga gtactaatat tcaggttata
    17221 cagatattta aacaataccc tgcaatttac aaagtaatac tcattgcgac ttcttaattt
    17281 aagaaagcgc aaggaacgtt tacccttgcg cttattataa taagtcaggc gtcgacttta
    17341 tccagcggcc atgaatcaaa cagatagcca tcaaaataaa tatcgtcaac gttcgagaat
    17401 tccagcagac gttgcttaac gttttccaga tgctgccaca ttgccagctt cgccgctcgg
    17461 gcatcttttt tgattaacgc ggcgaggatc tgtttgtgat cgcccaacca ctctttgcga
    17521 tacaggctgt catccagatg gctgtgcaac tggatccaca ttggattgtt ttcccgccac
    17581 tgccaggact gacggaacag ctccaccagc atgctgttat gcgttgcttc ggcaatagcg
    17641 agatggaact gcatgtcacc gctttcgctg ctgcctggcg cactggaagc cagttcacgc
    17701 tcttccagtt gcaatgcctg acgcattttg acgatatctt cgcgggtagc ctgcaaagcg
    17761 gcaaactcgg cgatgttgct ctccaataac tgccgcgcct gtaacagctc aaaaggaccg
    17821 gcatcgttgc agacgttggc atccggactg tctgtgttct ggctgcctga gttatcaaga
    17881 acatagatac cggcaccccg gcgtacttcc accagccctt tgatctccag catgatcagc
    17941 gcttcacgta ccaccgtccg cgtgacatca agcatttctg caatttcacg ctccggcggc
    18001 agccgttcgc cagggttgta cggcgtcttt atgatcagat cgcggatcat cgccccgact
    18061 tcctggtaag gtctttgcgc agaggtggca gatttcatgg tgttgtgtcc gttaggtgcg
    18121 gttaatcaaa tttttgccgg atgcggcgtg aacgccttat ccggcctaca acgggcaatt
    18181 ttacggcccc cgttaatctc aaactatacc attagcactg gaagttggca cgtacaggtg
    18241 ggcaaaaaaa cgaaaactgg tcaaccaaat atcagtttga ttgaccagta agtctgtagg
    18301 gtaatcagtt agttacagcg cagccacaca ctcgcgcgca ccgcgttcgc acagctgctg
    18361 atatgccgcc gtcactgcgc caacaaagtc ggcattctgc ggcagatcat cggcaaaaat
    18421 accgctcagg cccagcagcg ctttcacgcg gtctgcgccc tgatactgcg cgttaatctt
    18481 ctggaactcg gccagcatcg ggtcgaccac gtcaatggca ttaccctgct catccacgcc
    18541 ctgggtgtaa cgcatccagc cagccacgcc cagcgccagg tgacgccagc taccgccgtt
    18601 ttgcaggtgc agacgcaccg gatccagcag acgctgcggt aacttctggc tgccgtccat
    18661 tgcaatctgc caggtacggt gacgcagaga tgggttgctg aaacgctcga tcagcagcgt
    18721 cgcataggcg ttcaggtccg taccttccgg catcgacagc gttggcgctt gttcctgcat
    18781 catcagggca aaggccgctt tgcgataatc cgggttagtc atggtgtcgg caatagtttc
    18841 atagccgccg aggtaaccga ggtacgccag gaaagagtgg ctgccgttca gcatacgcag
    18901 cttcatcatt tcgaacggca caacgtctgc aacgaactgt gcgcccactt tatcccagtc
    18961 cgggcgacca ttaacgaagt tatcttcaat cacccactga cggaacggtt cgcaggcaat
    19021 ggcgcacggg tcgtaaacac ccagctggtc agcaatttcc tgtaaggttt ctggcgtcgc
    19081 cgccggaacg atgcggtcaa ccatggtgca tgggaaggtg acgttctctt caatccatgc
    19141 cgccagctgc ggatcgcgtg cctgagccag tcccagtacc gcgaccttcg ccacatgacc
    19201 gttttcacgc acgttatcgc aggacatcac cgtaaacgct ttcaaccctt tttcacgacg
    19261 cagacgcaag gcttcgacga tgtaaccaat cgcggacttc ggcgcagtcg ggttttccag
    19321 atcgtgcttg atcagtgggt tattgagatc cagttgaccg cttgccgcat cagcgcagta
    19381 gcctttttcc gtgaccgtta gagagacaat cgccgtttgc ggacgcgcca tcgcgttgag
    19441 aataccttcg cagccatcga tttccggatg cagcgcttct ttcatcgaac cgataatttt
    19501 cagctcggtg ctctctgcgc ctttttccgc tacggtgtac agcagttgct gttttttcag
    19561 gttttcgatc agcacgcggt cgttgcctgg catcaggtta acttcgcaga tgccccagtc
    19621 gctgtcggtg ctttccagca gatgatgggt atacagcgcc tggtgcgcgc ggtgaaacgc
    19681 cccgcaaccg agatgcacaa tgcgtgattc cagacgagaa tgatcccatg acgggcgggc
    19741 aaccggcaga ttgctgtcaa caatagtagt catcgtaaac tccagaatcc gtcttcaaag
    19801 agtgacggca aggaagaagg aaccggcacg gcgcgcagcc atgccggtgg atattaacgg
    19861 ctaaagaaag cgcgctggat cgccagttcg acaccgcgaa cttcggccag gcctttcaga
    19921 cgaccaattg cggagtaacc tgggttggtt ttcttcttca ggtcgtccag catctgatga
    19981 ccgtggtccg gacgcatcgg gatcaggtct tctttgcctt ccgctttacg acggtgttct
    20041 tcttcaacaa tcgctttcac cacttcgtac atatcaacgt caccgttcag gtgcgccgct
    20101 tcgtggaagg ttttcgggtt atcttcacgc atggtggagc gcagatgggt gaagtaaata
    20161 cgcggaccga actgcttgat catatcaacc agatcgttgt cagcacgcac gccgtaggaa
    20221 ccggtgcaca tggtaaaacc gtttgccatg ctgtttacgg tatcaaccat ccactgcata
    20281 tcttcaatgg tggaaacaat gcgcggcagg ccgaggatcg ggcgcggcgg atcgtccggg
    20341 tgaacagcca tacgcacgcc aacttcttcg gcaaccggaa taatcgcttt caggaagacg
    20401 gcaaagtttt cgcgcagttt ggctttgtcg atatctttgt acagctccag gtgcttacgg
    20461 aactggtcga gggtgtaccc ttcttccgcg cccggcagac cagcgatgat gttacgggtc
    20521 agacgcgctt tgtcttcgtc gctcatagtg gcgaagcgaa cagcagcctg agcaatttct
    20581 tcttcggtgt aatccgcttc cgcgcctgga cgtttcagga tatgcatttc gaatgcagcg
    20641 aattcgatct ggtcgaagcg cagagctttg gagccgtctg gcagcacgta ttcgaggtca
    20701 gtacgggtcc agtcgagcac cggcatgaag ttgtagcaca cggtgcgaat gccacactgc
    20761 gccaggttgc gcagagtctg ctgatagtta gcgatccact gctcatagtt gccagtgtgg
    20821 gttttgatat cttcgtgaat tggtacgctt tctacgacag accacaccag gcctgcgtct
    20881 tcaacgatcg ccttgcgttt gaggatctct tctacggacc atacttcgcc gttcgggata
    20941 tggtgcagcg cggtaaccac gccagttgcg cccgcctgac ggacatcagc taaagaaacc
    21001 ggatcgtttg ggccgtacca gcgccaggtc tgttccatga tttttcctct ttcacatagt
    21061 gggttccaca gcaaagagac gcggaaccct ggagttgttt aggtatccgg atgaacaacc
    21121 agaccgcagc cgtggcgttg catcggatga gatggcgtat aagttctacc ttttggtaag
    21181 acatgtcaac ccgaattctg aaattggtta accacatcac aagaatttta ctctcctggt
    21241 tgttgctgca aattgcagca aaaggttatc gatggttatt gatcagagga tgtgacattc
    21301 atcgcaacaa tggttgacca atttacataa catgtcgccc aaaataacaa cggttcaacc
    21361 aggtaaggta aaacgttatg catgtgctta acattctctg ggtagtattc ggcattggcc
    21421 tgatgctggt actgaatttg aagttcaaaa tcaattcaat ggtggctttg ttggtggcgg
    21481 cgctgtccgt cgggatgctg gcgggcatgg atttgatgtc gctgctgcac actatgaaag
    21541 cgggcttcgg caacacgctg ggggaactgg ctatcatcgt ggtgttcggt gcggtcatcg
    21601 gtaaattgat ggtcgactcc ggcgcggctc accagatagc gcatacgctg ctggcgcgtc
    21661 tcggtctgcg ctatgtacag ctgtcggtga ttatcatcgg cttgattttt ggtctggcga
    21721 tgttctatga agtggccttt atcatgttag cgccgctggt tattgttatt gccgccgaag
    21781 ctaaaattcc gttcctgaaa ctggcgatcc cggcagtagc agctgccact accgcacatt
    21841 cactgttccc accgcagccg ggtccggtgg cgctggtgaa tgcttatggc gcggatatgg
    21901 ggatggttta tatctatggc gtactggtga cgatcccaag tgtaatctgc gcaggtctga
    21961 tcctgccgaa gttcctcggc aatcttgagc gcccaacgcc atcattcctg aaagcagatc
    22021 aaccggtaga tatgaataat ctgccctctt tcggcgtttc gattctggtg ccgctgatcc
    22081 cggcgatcat tatgatctct accaccatcg ccaatatctg gctggtaaaa gatacccctg
    22141 cctgggaagt ggttaacttt atcggttcct cgccgattgc aatgtttatt gcgatggtgg
    22201 ttgcattcgt actctttggc accgcacgtg gtcatgacat gcagtgggtg atgaacgctt
    22261 ttgaaagcgc ggtgaagagt attgcaatgg tgattctgat catcggtgcg ggtggcgtgc
    22321 tgaagcagac tatcatcgac accggcattg gcgacaccat cggcatgttg atgtcccacg
    22381 gcaatatctc gccctacatc atggcatggc tgatcactgt gctaattcgt ctggcgacgg
    22441 gtcagggtgt cgtttcggcg atgaccgccg ccgggattat aagtgctgca atccttgatc
    22501 cagcaactgg tcagctggtt ggcgtgaatc cggcgctgct ggtactggcg acggctgcgg
    22561 gttccaacac cctcacccac attaacgatg catctttctg gttgttcaaa ggttactttg
    22621 acctgtcagt aaaagacacg ttgaaaacct ggggtctgct ggagctggtc aactccgtgg
    22681 ttgggctgat tattgtgctg attattagca tggtagcgta aaaggacacc agagcctgcc
    22741 aatggcaggc tctgactgat gacaaagtct aaaaatgtgc ccagacagtc ctctcgcccc
    22801 tctggggaga gggttagggt gaggggaaaa gaccagcaaa gtgcgctttg tttatgccag
    22861 atgcgacgct aacgcgtctt atctggccta caaagggcta acgtgcaggt ttttagcttc
    22921 aggtaatatt gcgtacctgc attagcaatg ccctgtgatt tctttattga taaacaaaag
    22981 tcacgccaat aatcgattgc acattccctg cagtcacctg ccctccggta cgtgcgtaat
    23041 ttgccgttaa tcccagactt accgccgaag tccctactgc tcctaacgat accgtgttat
    23101 tcgctggaat aatcgtaccg ttgcgcgtca actgtacgcc gacgccctgt gcaggtgaaa
    23161 acgacgcggt attggtgaaa atcgagttgc ccgcatctgc ggttgtgccg gagaggtaat
    23221 accccaggtt ttggcttttc gcacaataaa cggtaagagg aattggcact gaaccagggt
    23281 agtccggcag agtaacggtg acatcacgag cagaaacatc gcagccgcca gtaggcacca
    23341 ccacatcatt attggcgtaa atattccaca caaactggaa atcatcgctg ttatagttgt
    23401 tggtctgtcg caaaataagc acggcaatta atgagccagc tttaatcgcc accccgcccg
    23461 cactgctcac aggcgtcaaa taaagcgcca ccggccacgg cttatccgtt ctcgaattat
    23521 aaacaacgcg cggcgtttcg ctggtggtcg gaaatggata gctactgcca ctatatttta
    23581 cggtcccgga aaaattagat aacacgccgc cataagccga gcctcgttgc agtgtgacat
    23641 agtctgtaat ggtttccgga taatcgttat ggcaaaagat ttgcgtcgaa agatctacga
    23701 ccaggttttg ccccacattc acggcaggcg caaggtttac ataaacatta gcgctgccac
    23761 cgccaatagg gatagctgta ccattggcgg ttttacaggc gaatgaccag gcatttaccg
    23821 accagcccat cagcagtaca gcaaacaggg taataactcg tttcattaca atcatctctt
    23881 cgggttcagc tgtaggtata ggtgatgcta atcactgcct gaatggttcc ctgagtggct
    23941 ccgccattta ctgtcaatgc tctgacctgt aacgggaagt gcgctgattg tgaggaatca
    24001 tccacctgaa ctgttttggt tgcgccagta ttcaatgtgt tgccactgtc atcctgtagc
    24061 tctaactgga tgttttgcgc ggtcccctgg tttttataat atccggtact gtcggctgcc
    24121 ccgctgaagc tggcagtgac cctcgacgtt cccaccggac aattagtcaa ctcaagcgca
    24181 acatcatgcc aggccgatgc cgccccggca gacatcagac tgaaagaata aagatcgccg
    24241 agatcaaccg tggcattggt ggtggaaact gtgcacggtt tggcgacgac cttaccgttc
    24301 accgtgatgg tgacatcggc tgcctgtatc gtcgcacttg cgagcgccaa tattgccgcc
    24361 aatacatacc cacgtttgca ccatttcatg agcatctcca gttactgata ttcaagagtg
    24421 aaggtagccg tggcattgat atgccccgca gtgacaggca cctgtgtcgc cattagccgg
    24481 gcgtaaaaat tcaacgtatt tggtttaccc ggcgtcaggg tcgtccacga aatcgcggac
    24541 gatggagcat taaggggtat ctgattttgc tgctcattca gaagctgtat tcccagtccc
    24601 gaagccgctg ataccgtatt ttcaagtgca agcaggttgg cattgtggct atctgcaacg
    24661 ccggtaaacc caacctttac ggcagaaacg gcgttaccac agggtgacag caaaatacga
    24721 aatggaacga caggagtcgt tgcgccaatg ttgttaaatt gcttcgccgc gttttccatc
    24781 agatcaacag taaaattggt tgattcagcg gccacactac agccgttatc tctgacatag
    24841 ccgcggatag taatcgtgct atccgcagcc aaaacgcgac ttaccgccag ccacaaaaaa
    24901 gcgcacagaa gataaaaagg tttgtttctc atcacgcccc cttaacgaca ttcagctgat
    24961 agctgggtta ataactgctg ctgactctct ggtggcagtt gataattggc gatacagtga
    25021 gcattttcct cttctcccca tttcacctga acttttcccg ctaaaggcat tccgctgagg
    25081 taaacctgac cattatccgc aacaatgccg ctactctggc tactctctga tgtcaccatc
    25141 gccccaaacg gcagcggctt attattgtgg gttagcgtca tgagcagttt tatcccaacg
    25201 cgcgctttaa actctgctcg cacgatcgcc ccacgagtgg gaacaacgtt agcgaccgcg
    25261 ttatctaaat cgacgttatc agccagggta ttggtatcca gcgccactct attttcccga
    25321 tattcagtgg cataaggcag cacggcataa ccacgccagt cggtacgcac ccccgtctgg
    25381 ttttcgactt ttgcatcttt tgcgccaggc gctttaacaa gcaccaccgt atcgtttaac
    25441 ggctgcccca gcgttacgcc attggcatga gccagtaccc caccgctgac tccgtaatag
    25501 agctgcttaa tatcatcact atggctgtaa ccgatattgg cattgccgta accaccgcga
    25561 taattcagcg tggcgtagcc tgtgcttccg ctattaccat cgcctccccc ggcatagccg
    25621 gtttgcacgc tatagctgag gttgttgtct tccagcaacg taccgtatac accagccaga
    25681 ttggtcattc gaccgttgag atcgtgtgac atgctgtagc tggcactggc atgtcgccac
    25741 tgagatttac tgtcagaacg cagccagtgg ctgaaaggaa tattgacgtt aagcgctaac
    25801 atctgatcac gtcctttttg ccaggcgttt ttcgtcaggc tatagctgag cgtccagttg
    25861 atatcttcga acgcagtatt taatccagcc tggaattgct catcgacatt actcgttccc
    25921 caataagttt gatggctacc actcaaatac agtgttgatg agcgcccgag ttgctgagta
    25981 acggtgagtt gtaatttccc gcgtttgtta taagcgaggt tgtaatagtc ggtgaatttc
    26041 ggcttaacct gaataactcc gtcctgtgtt tcgatgttgt agccattcat tcgactgtat
    26101 gttgtatcag cgaaattaaa atatccgctg gtcgaataac ggtaacccac taactgaata
    26161 ttcgtgcctg actcattgag cgatttgtta tagagaaaac gcaccgattg tccgtcatgc
    26221 tgactgtcat cgggaagtgt ggaattagcc tgcgtcatat ccacagacag agcgcccagt
    26281 gcccccatgt ttttcccgat accgaaatta aaagcacgat aacgatccgc cagttgcgtt
    26341 ccaccatata ttgtccagcc agccggaagg ccgtggagta atgtactctg gaaaaagcgg
    26401 ggtttttcct gctgcgcatt tccactacgg tattctcctg ccgtaatgga ataacgagta
    26461 tgcccttcac gttgcaaaag cgggactgac gaatagggta cggtaaaaat ctgcgtgctg
    26521 ccgtcagcct ctttgatcgt tacctgcaag tcaccactat tacctgcggc atagatatcg
    26581 ttgatggtaa aaggccccgg tggcaccgta ctattataaa tgtcataccc attttgttta
    26641 atagtgacct gtgcagtacc acgagcaata ccgtggatca ccggggcaaa tcctctttgg
    26701 ctatcgggta acatattgtc atctgaggcc aattgtacgc cgcgaaagtt aataccatcg
    26761 aaaatatcgc cctgagtata accatcaccc agcgtcagcc gggaacgtaa cggtattatg
    26821 tctcgctcaa gccaggtatt gatatgctgc catttatttt tgctacctga tgatctgtcg
    26881 ctactgttat aactccaggt ggtattgtcg cgtaaacgcc acgcaccaat atttaaccca
    26941 ctctgtaggt ttaaatatgc ataatggctg ttacccccaa tccgattctg tacactattt
    27001 ccgctgaaat tataattgag caatccggca ttaataccgg gatcccataa ctcaggagga
    27061 atataaccac gcgcgcgatt actcataaat gcctgaggga tcgtcaggtt gagtcgctgc
    27121 tgaccaacat ctaaatgcgc agtagcgtca tggatcattg aggttaatgg cacgcaggca
    27181 tcatccgcca gcagattcat accggagaca gaagccgtat tcagccccat actggcaagc
    27241 tgcgcgcgtg tcaggcaggg aacaatccct tgttcactgt cgcccgtatt aaatgtgaca
    27301 tcacgcgttg ccatataacc attattcaaa tagatatcga cacgatacgt ccctggcggt
    27361 aattcttgcc cattttcaaa acgcgataaa tcagccacag cctggggatc atccgctaaa
    27421 aaacgcgggt taaaatagag ttcggcagat gacaaaggtg cctgtgcggc aaaagcacag
    27481 gcgacaaaga gccggacaaa aaaaccagcc aaacgatgct tacgaatatg caagcattgt
    27541 gtgtttcgct ggtaaagtct taaattcaga tatgacatta ttcctccggt ttaaaatcac
    27601 ccggcagtca attcttttta ttcaggcgaa aaattccccc tgcgttattc cattacgccc
    27661 gtcattttgg gggtaagtgc gccataatca tttattgttc ggtaagtaat attgcttccc
    27721 gcatcagaag gcaatttaac cgtgctttcg cccattggag gcaccaaggc attttcaaga
    27781 acccgggctc cggcattcaa ctctgttacc gtcaggtaat agggtgtcgg gttaatcagc
    27841 gtcagagaat tcgcgctacg acgaaatctt aatttttctg cggcctgatc gggtggcaac
    27901 gctaatttag ccggacgata gtacagttta atgcggctga taattgcgag ctgtagcgta
    27961 ttctcagtca attttgattt atccattgac ggaatcgctt taacgttcat ccagaataaa
    28021 ctttcccggt cctgtggcaa ttggttattt gttgcatcaa gaatacgcaa ggtattctct
    28081 ctttttccct tcatcgcaaa cagaggaggc gtcacgataa aacgaccatc ctttacacca
    28141 tcggcatttt ccacccatga ttgaattaaa taggtgctat tttcatcatt atttgtcacg
    28201 gcaagttgca cttgtttttg ccctgccgga taaattacgc gagtcgcacc taaggccact
    28261 cccgcttcag cgcgtccggc aaccatcatt gccatgaata tcaggatacc tgccagcaag
    28321 cagaatgtta tttcctgcga tttccttaca ttgacgtttt tattactcac tgtcctgttc
    28381 ctgttatcac attatctgct gaacaattac tgataggtta aagagaacca ggcctgggca
    28441 ttggcgatgc cgccagtaac ccgacgcccg gtagcacgat atttggcgat gaaatgtagc
    28501 gaagtagagc ctgagtaaag ccgtttccag tttgctggag gacgattaat cggtacgagg
    28561 tttccttcat catcaaacaa cgctacgcca atattggtgg caatccctgg cccctctccc
    28621 acggaaagca catccggatt tttaccatcc gcgacaccgt gaaacgccac acccacacgt
    28681 tcactcacca ccgtgctaca ttcctgtaaa tgaataacaa aaggcaccgg tgcgctatct
    28741 tcccctaccg catgaaaccg gttactgctg atttgcccca tattgaccgt catttgttta
    28801 tcaccggctt caatccggca agtttccgca ataatgacgc cctgaaattg catatttccg
    28861 ccgggcagcg tggtgttcca tttatttccg gccagagcaa acattggcag caacgacgct
    28921 aatagaaata atctcttcct tttcatcgtt atttttatcg cacaagggtg ggcatccctg
    28981 cccgtaatga cgtccctgaa cctgggtagg ttattgatac tgaaccttga aggtcgcatc
    29041 cgcattagca gcacccgagg ttgcggcacc ggttgcaaaa taacgcgcct ggaacggaat
    29101 ggtgttggtt ccgttattca gggttgtttc tgaactaaat gtcgcaccgt ccagcgccag
    29161 cgcagcaccc gttctgtcca ggatctgcac accaacgttt gttgcgctac ccgcagctga
    29221 actctgcaga gccagaacgt tggtatgacc cgcatcaatc gccgtaccta aaaaggcaac
    29281 agcggcttta gatgcaacat tggtatcgca atcattcagc tgaatgttaa aaccgacagc
    29341 agaactggtc gctccttcct gtgccagcga tgcggtacga acctgtccta actgaacggt
    29401 ttgatcaaca gagcctgcat caactgcgca agcggcgtta acaacttccc ctttaaagtg
    29461 aacggtccca ccatttaccg tcgtggcagc ggccagagcc gctgtagaac tgagggacag
    29521 agccgacaga acaacgattg ccagagtttt aattttcatg ctgctttcct ttcaaaaaac
    29581 tatttctaaa tcgacatggg cagtcgttct gtacactttg ttttgtcaac gagtttgact
    29641 gccaacactg cacagttttc ccccaaaaga tgaaacattt ggggccattt tgactcatag
    29701 aggaaagcat cgcggacaaa ctttttcagt ttatttgttg gcttaatatt ctattgttat
    29761 ctttatttat agatttttat attgcatgag gtggtttttg gagagaagaa tgaggaagat
    29821 gcgtcgagcc acagaaacgt tagctttaca tatagcggag gtgatgtgaa attaatttac
    29881 aagagaaata atttacatat caaacagtta gatgcttttt gttgtttttt aatattttta
    29941 tgcttgagaa aaaatacgta acttatttat gatatggaca gtttggcccc aattgtcttg
    30001 tatttatttg ctttttaact ttattatcaa taagttaaat taaacttctt ctctttttaa
    30061 tttttcgttt atgagattat ttctttccca taatccggca aaacgagcag cattactggc
    30121 ggtataacgc acagtatggc gaatatttcg atgcccgaga taatcctgaa ttaaacgggt
    30181 atcggcacca cgctccgcca gttcataacc gcaagcatgc cttaacatat gaggatgagt
    30241 ctgcgttacg gttccagctt caataccggc atcgcgaata atgcgatagg cctgctggcg
    30301 agaaagccga ctcccgcggc gagaaataaa tatagcgtca gtccggtcag cgcctttcca
    30361 gttagcacgt tcctgggtcc atcgttccac ggcttcacgc tcatcaaaac gtaacgggtg
    30421 aacggtagaa aatccgttct tcagtcggcg aatatttatt ctaccttcat taaggtcaag
    30481 gtcctgataa tgcagatcaa gcagttcact aatacgcatc ccatgccgat atgccaacag
    30541 aataagacaa taatctctgg ctcccgttgc cccgtaacaa accgcctgca tcatggcctg
    30601 aacttcttta ccggtaagat aacgacgttt actcacaata gtagtactcc taactgagat
    30661 atatttgaat gaatacctat aggaaacctc aatcggtcaa aattagccca atagataaaa
    30721 agataaccac acacggagtg atgtggctat cagtcaatta cattaaatat catacaaaaa
    30781 taaaatatca ctgatgtgac aacatctatt ttcattgatt tgattcaata taacaataga
    30841 ttgcagtgga ttaacttcca gttgcttaaa aaataaagaa aaaagtgcgg aaacaggaga
    30901 atttaagtga tgtgtaaatt tatgaatgat tttgtttatt tattttgaag aacataaacc
    30961 attgtatttt gtatggatat ggttaactta atgtttatta tgataattaa gaatcacact
    31021 tacaataaca tcaattaata aatggttaat catttttgat atatcgtaag aataatgtag
    31081 ttttaaacac catccctggt atctcaacta tctctataaa acagcgtgac gctgtcgtcc
    31141 tctggctcta tcccagatgc cgtaaaaacg ccctgcattg ctggcggtat accagacagt
    31201 atgacgaata ttgcgatgcc caagataatc ctggataagt cgcgtatcta ttcccatatt
    31261 cgccaaagca aaaccacacg aatggcgtaa catgtgcgga tgaatctcca gtgacaaccc
    31321 ggcattacca ccggaagtcg agataatatg gtaaaactgt tgccgagaaa gcggatttcc
    31381 cttacgagat aaaaataccc actcgctctc agcatgcggg tacgaagtac ggatactcaa
    31441 ccagtttttt aaagcctgaa cttctttgtt caatagcgga tgcgttgttg aaaagccttt
    31501 ttttaatcga tggatatata tacactttgc cttaagatca atatccgaaa tcctcaatcg
    31561 acaaatttca ctcgcccgga aaccatgaat aaaacaaagc aaagtcagac aataattacg
    31621 tgctgcatga ggcccggtat ttgctgcttt aaggagtgat tcgatttcac tatgggtcag
    31681 gaagttcctt tttttgttat cagccttatt cttcatcgtt tttcccttag aattacagac
    31741 gcgcactagc tgtgctgggt tagcaataat ccaacatttt tatcctgatt atgtttataa
    31801 aagcgaacgt ttgcttaata actaacgtaa gtggaccagt tcttctgagt gaacttaaat
    31861 ggagtagcga tagttactta taataacttt gccatacaaa cgccatgctc ttgcatgcta
    31921 tgtaccttta tatatttatc aatcggatac gaatctaata ataaccctca tcattaatgc
    31981 aactaatctt atctatatat catgtgatat gttctgtaac aagtaaaatc aacataaaaa
    32041 attcagcaaa atttaatatt cgttcaacta gccgtctcac atataacatt attagtcaac
    32101 tccatttacc acaaaatggc tatttcatgg aacgcagtaa tttcttagtg gctattatca
    32161 agctaattct taggtcccta gcgattattc ctggctggtt cgtcagttta ggtcatgaga
    32221 ttgttacatt cctttttatc ctggagatat gggggaacgc aggcgacgct ttttcatcta
    32281 catccgttgg taaatatggc tttggtcact acgacttaaa ccctcctcaa tcacagctac
    32341 tcgccgggcg atatggcaaa caaactaatt gaaacatcat cctgaaaaca ccagcatcaa
    32401 caagcctctc cagatcgaca tcaggagtga ccagttacaa gccacaaacc aagaaacata
    32461 tttcatgatg agaattatgc tcagcctgat ggcgggtaaa cacttatttt caccaagctc
    32521 actgagttag cttgcaaagc tttctttgtt tagattctaa ataaattatt tataaaacat
    32581 atagttaaaa gaaaatcttt agtttttgaa gaccaccgca agtgttcgtc tggcttcaca
    32641 tggcatctgc ctcttagaaa aagatcgaca tattttgtga cacgaattgc aaatctggtt
    32701 ttgttgtatg gattgcgtga tttttgatct ggtataacag gtataaaggt gcaccacgat
    32761 agtcaatgaa actgggcatc tcgcaatcta tggcaaacat cacttcagtt ctttctcatc
    32821 gggtgatgaa aacgcacttc agtctgaaag gaatatgaaa atgagatcaa cagacactct
    32881 attttatgac tctgggtaaa atggattgag taagcgatat agcttacgaa aattcaaatc
    32941 aattaaacat cagcagagat tttatactca ggtatttaat ctggctctct gtttatttaa
    33001 ataatgtgga aagagatttt tcacaggaga cattatacaa aaaatataaa atacagctac
    33061 ccgttgccaa agacactata agcctggcaa aaaatattac acaacataaa tgctaattgt
    33121 ttatgcgggc tttgtattgc tttctgtatc ctacaaatga gtgaaattta tgaaaaaggc
    33181 taaaatactt tctggcgtat tattactgtg cttttcgtcc ccattaattt ctcaggctgc
    33241 gacactggac gtacgtggtg gatatcgtag tggaagccac gcctatgaga ctcgactcaa
    33301 agtcagtgag ggatggcaaa atggatggtg ggcaagcatg gaaagtaata cctggaatac
    33361 cattcatgat aataaaaagg aaaatgccgc actcaatgat gttcaggttg aagttaatta
    33421 cgcaattaaa cttgatgatc aatggacggt acgcccggga atgttaacgc attttagcag
    33481 caacggcaca cgttacggac cctacgtaaa actgtcctgg gacgcgacaa aagatcttaa
    33541 ttttggcatt cgctatcgtt acgactggaa agcttaccga caacaagact tatccggtga
    33601 tatgtctcgt gataacgttc atcgttggga tggatatgtc acttaccata ttaatagtga
    33661 tttcaccttc gcatggcaaa cgacgctata cagcaaacag aacgattatc gctatgcaaa
    33721 ccataagaaa tgggcgacgg aaaatgcatt tgttctacaa taccatatga cgcccgatat
    33781 tacgccatac atagaatatg actaccttga ccgtcagggt gtttacaacg gcagagataa
    33841 tttatcggaa aacagttatc gcattggtgt gtcatttaaa ctgtagtaga caggagacag
    33901 tcacaatgaa taaaacaata acggcgcttg ctatcatgat ggcttcattt gccgcaaacg
    33961 cgtctgtatt accggaaact cctgtgccat ttaaaagtgg taccggagca attgataacg
    34021 acactgtcta cattggttta ggtagcgcag gtacggcatg gtacaagctg gatacacagg
    34081 ccaaagataa aaaatggaca gcgttagctg cattccctgg tggaccaaga gatcaagcaa
    34141 cctcggcatt tattgatggt gatctgtatg tgtttggcgg cattggcaaa aatagcaagg
    34201 gcttgactca ggtatttaat gacgtacaca aatacaaccc caaaaccaat agctgggtta
    34261 aattgatgtc gcacgcgcca atgggcatgg cgggccatgt gacttttgta cacaacggca
    34321 aagcttatgt tactggcggt gttaaccaga atatcttcaa tggctatttt gaagatctca
    34381 acgaggctgg aaaagattca accgctatag ataaaatcaa cgctcactat tttgacaaaa
    34441 aagcagaaga ttatttcttc aataagtttc tgttgtcttt tgatccctca acccagcaat
    34501 ggagttacgc tggcgaatct ccctggtacg gaacggctgg tgcggcggtt gtgaataaag
    34561 gtgataaaac ctggcttatt aatggcgaag ccaaacccgg attgcgaacg gatgccgtat
    34621 ttgaacttga tttcaccggt gataatttaa aatggaatag gcttgctccc gtctcatcac
    34681 cagatggcgt cgctggcggt tttgcgggga taagcaatga ttctcttata tttgccggag
    34741 gggccggatt caaaggttca cgagaaaatt accagaacgg taagaactat gcgcatgaag
    34801 gcctgaaaaa atcatatagc actgatattc atctttggca taacgggaaa tgggataaat
    34861 cgggtgaatt atcgcaaggt cgggcctacg gagtatcatt gccctggaat aatagtctat
    34921 tgattattgg cggtgaatct gcaggcggca aagcggtgac ggattcagtt ttgatctctg
    34981 tgaaggataa taaagtcaca gtacaaaact aacgcttcag ggccccggta aagggccctg
    35041 ctatcaattt gtcatttcaa ttacgcgaaa attatatgaa cgcaataata tcgcccaatt
    35101 attactatgt tcttaccgtt gctggtcagt ctaatgccat ggcgtatggc gaaggactgc
    35161 cattaccgga cagggaagat gcgcctcatc ccagaatcaa acaattagcg agatttacgc
    35221 atacgcatcc cggaggcccg ccatgtcact ttaacgacat tattccactg actcactgcc
    35281 cacacgatgt tcaggatatg cagggttatc accatcctct ggcaacgaat catcaaacac
    35341 agtacggcac cgttggccag gcactgcata ttgcacggaa attactgccc ttcattcctg
    35401 ataatgcagg gattctcatc gtcccgtgtt gccgtggcgg atcggctttt accgcgggca
    35461 gcgaggggac atattcagaa cggcacggag ccagccatga tgcttgtcgt tggggaacgg
    35521 ataccccgct ctaccaggat ttagtcagca gaacgcgagc cgcactggca aaaaatccgc
    35581 ataacaaatt cctcggcgta tgctggatgc aaggcgaatt tgacttaatg accagtgact
    35641 acgcgtcaca ccctcaacac tttaatcata tggttgaagc ctttcgtagg gatctaaaac
    35701 aattccattc tcagcttaat aatattactg ccgcgccgtg gttttgcggc gataccacct
    35761 ggtactggaa agaaaatttc cctcatgcgt atgaagctat ttatggcaat tatcaaaata
    35821 atgttttagc caatattatt ttcgtcgact tccagcaaca aggtgaaaga ggactgacga
    35881 acgcgcctga tgaagatccg gacgatttaa gcacgggata tttcggttca gcgtaccggt
    35941 cagcggagaa ctggacgacg tcactgcgaa gcagtcattt cagcgcggca gcccgtcggg
    36001 ggattatttc tgacagattt gtagaagcaa ttttgcagtt ttggcgcgaa aggtgagcgg
    36061 ggagtttatt catgggggat gtccttgtcc attcccagag cagacaacaa aaaacaaatg
    36121 agatcaaggt aatatctctt cacttgcggg ctcgaacagc aggcaattgc caatataata
    36181 atctggcaca agggtgaaaa acgcgcaccg cataaaccgt tgttattgct tacagattag
    36241 ccggatgcct taatagacat ccgcgccttt tcggttatgg ctcggaaatc tacgaacatc
    36301 tacacagttt actggaacgt tttgggccac agcgttcgca gcaccgtcct gatatgccgt
    36361 tctggcgatt ataagtcgac ggattctggc aaatagacga tgatgatatg cttaatgatg
    36421 ttcaaagaaa ttaggccatt attatattat tgcttgcatt ttctttcatt cctaaaagca
    36481 cccgccatta atcccaggcg cattatgcaa aggaaaatga ttaggtaaac aataagattt
    36541 ataatcagga aaggcgactc gcaataaaac ggaaagtcgc cttaacattg gtaaaatatg
    36601 ccctttacta ctcctgtatc atgaacggct catcacattt aaagctgacg ttgatccaga
    36661 ctaccatata ttgcctttat ttcatttttt acgccttcgc cacggtacac caaagacgta
    36721 tcgtatcgtt gatacttatc ttcccgtgca gcactgaacc agttaaaaga tccgacacac
    36781 agcagttctt catcctcaat cacaattttg ctgtggacac gattaaccaa tttcgtagcg
    36841 atacccatct tattgagctt ttcaacagca tcattcagca gatgttgttt ctcctgacgc
    36901 ttatcatcat cgacatgcgc tatattgcag tttttatccg tcaccacggt aatatcaatg
    36961 ccccgggaac gggccaaagc cattgatgcc agaaatcccg tttgctccac tttttgccaa
    37021 gataaccaag gggaaataat cgttattttc ttttgagctc cggcaagagt cttgttcaaa
    37081 aatccatcat gctgctccac accatgcagc gttgaaattt gtgtatgtgc ggaaattaaa
    37141 tcctgtcgtt tctgaaactc aaattgcaac gcattattat ctgaagaaaa gagatatttt
    37201 gccaataacc ctcgcggcga aaatgcgggc tgcatctcga taaggtccat atcgccaaat
    37261 accagaaaac tatctttagc gcgtgagaca gcaacattga ggatactgct gttgctatca
    37321 agaaacctgc catcttcatg ttttgagtag acaggagaaa agagaacaat cgccctttcc
    37381 gccccctgca aggagtgcac agtccccact gtcagtgaac cctgctcgtc tttgccatta
    37441 atttccagct tacgcaatga cgttttgata gcattaacct gcgccgaaaa tggcgtcaca
    37501 acaccaacaa ctttgtacag tggttccccg taatggcgct ctatatcatc cttatgtgcc
    37561 accagccatg ctgctattgt ttcagcttca agagggttat aacggcttcc tccatttggc
    37621 ttcatgcctc taccatcaat atgtaaatat cccattgcgg ggaaaatcgt ccctttttcc
    37681 atccctcttt taggctgcaa cttaccgtga tagcaaagtg agttacagta gccgatgatg
    37741 ttattgaaac agcgacgatg ctcatacaga tacattccgc gctcgaactt gggatcatac
    37801 tggtagtgag agttgaactg ggctacctgc attacgctgc cagatgctgc actttttcca
    37861 gacccattta ttatcgaata cggtgaccgc ctgagcggcc acctttaatc gtgggcagtt
    37921 acacagaatt atttacaggg ccttgctggg agttcttaca cttgctcgcg atattcaatt
    37981 ggccattgtc aacaagatga cgcaggtttt gattggtgat atctatttac gtcagcaatg
    38041 cgatgtgttt tggctcggct ataccatcag tcccgcttat actcgccagg gatatgcaat
    38101 tgaagcaatt accgccacga ttgactggat aaaagagaac ggtttttcac tcatcaaagc
    38161 cggtgtgaat ccggaaaata ccctttcaaa aaaattactt atcaggattg gcttcaattt
    38221 ttcgagcatc gaagatgggt gagcatattt atgttctgga ttaaacaaaa ggagaataat
    38281 ctcccgaagg gaaaagccga tgaggccatt tcaggcgtac ttcaacatgc attcgtcagt
    38341 gtcatctgcc ttttccagta aatgggttgg cattcaattt ccaaagtaca tcacactttt
    38401 tctactgaaa catatccctc ctataactac gtgttaatat tcgctccgta aatacctttc
    38461 aactcaaccc gaggctttga tgctggagaa tgtaatcatc cgataatcag ttccccgacc
    38521 ttttcaggcc ggactgatta tcaatgcgcc gaaatcgaat gcggacaccg cggtgtgttt
    38581 gcacgttttg cacatgatga ttaaacaggt tcttcagtat gaatttatcc cgtcaggaac
    38641 aacgtacgtt acacgttctc gctaaaggtg gacgtattgc gcacgtccgc gattcttcag
    38701 gccgcgtcac ttccgttgaa tgctacagcc gcgaagggct gttgctgacc gactgcacgc
    38761 tcgccgtctt caaaaaactc aaaaccaaaa aacttatcaa gtccgtcaat ggtcagccct
    38821 atcgaatcaa cactaccggg ctgaataacg ttcgcgctca gctcgataat cgctaaggag
    38881 agtttcgatg acagcacacc acttcacttt tcaaatcaca gatgaaagtg atgccagtga
    38941 tatccgggaa gtcgaaaccc gcgcatttgg cttcagcaaa gaagccgatt taacggcctc
    39001 tttacttgag gatgaaagcg cgcgccctgc gttatcgctg ctggcccgac atgaaggcaa
    39061 agccgtcggg catattttat ttacccgggc aaccttcaaa ggggaaatgg attcaccgct
    39121 gatgcatatc ctcgctcccc tggcagtcat accggaatac caggggatgg gcgtgggagg
    39181 acggttgata cgcactggca tcgagcacct gaggttgatg ggctgccaga cggtttttgt
    39241 tctcggccac gccacctatt acccccgaca tgggttcgaa ccctgtgccg gagacaaagg
    39301 ctatccggca ccttatccca tcccggagga gcacaaagcc tgctggatga tgcaagcact
    39361 caccgctcag cccatgaacg tgaccggcca catccagtgc gcgcaggccc tgatgaaacc
    39421 ggagcactgg cgtgaataac aatcttgttg tgcccttttt aatgcttaac ccaataacag
    39481 gaattaacta atggatattc cacgtatttt taccatcagt gaaagcgaac accgcatcca
    39541 caacccgttc accgaagaga agtacgccac gctgggtcgc gtgctgcgca tgaaaccaga
    39601 gacccgaatt ctggacctcg gcagcggctc gggcgagatg ctctgcacct gggcccggga
    39661 tcatggcatt acggggactg gaatcgacat gagttcgctc ttcaccgcgc aggccagacg
    39721 ccgcgcggaa gaactcggcg tcagcgaacg cgtacatttt attcataatg atgcggcggg
    39781 ttacgtcgcc aatgaaaaat gtgacgtagc agcctgcgtt ggcgcgacct ggattgccgg
    39841 aggctttgcc gggacagtcg aactgctggc gcagagcctt aaaccgggcg ggatcatgct
    39901 catcggtgaa ccttactggc gtcagcttcc ggcaacggac gagatagccc aggcctgtgg
    39961 cgtcagctcg acgaccgatt ttctgacgct accagggctt gtcagcgcgt ttgacgacct
    40021 cggctacgac gtggtggaaa tggtactggc ggaccaggaa ggctgggacc gatatgaggc
    40081 cgcgaaatgg ctgaccatgc gccgctggct ggaggccaat cctgacgatg acttcgccgc
    40141 cgaagtcagg gccgagctaa acatctcgcc gaaacgccac gtaacgtacg cgcgggaata
    40201 ctttggctgg ggcgtgttcg cactcatcgc taggtaacaa caggttcccg gtatttctga
    40261 ctggagatac cgggggctta gcctttgctt taatctgatg catcacctgc acaatacata
    40321 ttcagtatgt ttattaatca agatgtcacc ataaggagca tcatatgtca caacatgaac
    40381 agttctgtca ggcttgcgga atgccgatgt cggcaccaga tgcacacagt gccagtgatc
    40441 aatattgtgc gtactgcagc tacagcaacg gcaatctcaa accctgggaa gaagcggtat
    40501 caggtttagc cagcttcctt gattcctggc aaaaagtcgg cccggaggaa gcacataagc
    40561 gtgcaaaacg ttatctgacg gcaatgccag cctgggctca taaagcggag agtgatttga
    40621 gctgattatc tatactccct ctgaatctat tcaggagatc cgatgttttc ttacattatg
    40681 ctgggaacca acgacctgcc acgcgccatt acattctaag atccgctgat ggagatgctg
    40741 gggtatccac gtgcaggacg taatgaagag ggagcgtcct gcaacaaagt ggtgcgataa
    40801 cagttaccgc gttttattca ttctcattat ttcaaccata atcactggtc tctctagccc
    40861 gtaactttca caaacgttga gcgcttttat tccactcggc tcacataatt ttttatttgt
    40921 tcagctttgc gcgttttcac gtgaacgttc tcacaattat tcgaccagtc tgcgcctata
    40981 aaacgctcaa cgagtgattg ttttacggtc tacggagata gctatgtcat tttctgtgca
    41041 ggaaaccctg ttctcgcttt tgcagcacaa tgcgatttca ggacacgaaa acgctgtcgc
    41101 tgacgtcatg ctgtgcgaat tcaggcgtca ggcaaaagag gtctggcgag acaggcttgg
    41161 gaatgtcgtc gcgcgctacg gtagtgataa acccgatgcg ctgcgactga tgatttttgc
    41221 gcatatggat gaagtcggtt ttatggtgcg caaaatagag ccttctggat ttttacgctt
    41281 tgaacgcgta ggcggtcctg cgcaggtcac tatggctgga tccatcgtca ccctcaccgg
    41341 ggacaaaggg ccagtcatgg ggtgtatcgg cattaagtcc taccactttg ccaaaggcga
    41401 cgagcgcacg cagtcacctt ctgtcgacaa actgtggatt gatattggtg ccaaagacaa
    41461 agacgacgct atacgaatgg gcattcaggt cggtacgcct gtcactctgt ataacccgcc
    41521 gcaactcctg gcaaacgatc tggtgtgcag taaagcacta gacgatcgtc tgggctgtac
    41581 tgccctgctc ggtgtagcgg atgctatcag tactacggag cttgatatcg ccgtttatct
    41641 ggtggcttcg gtacaggaag aatttaatat ccgcggcatt gttcccgtat tacgccgtgt
    41701 aaaacctgac ctggcgattg gtattgatat cactccatcg tgtgacaccc ctgatttaca
    41761 cgattattcc gaggtcagga ttaatcaggg cgttgggatc acctgcctga actaccatgg
    41821 tcggggaacg ctggccggat taatcacgcc tcctcgcctg atacggatgt tggaacagac
    41881 ggctcttgaa cacaacattc cggtgcagcg agaagtggct cccggcgtga taacagaaac
    41941 cggttatatc caggttgagc aggatggtat tccctgcgcc agtctctcta ttccttgtcg
    42001 ctatacccat tctccggcgg aggttgccag cctgcgtgat ttgactgatt gcatccgcct
    42061 attgaccgct ctggcaggta tgtcagcagc acatttcccc gttgagcctg attcaggcac
    42121 tacacaagag gcacatccat tatgaaaaag atccttgtgg catgcggtac cggcatgtcg
    42181 acttcaacca tgattgcaca caagctgcaa gagttcctga ctgaacaggg catttcagcc
    42241 actaccgccc aatgctgtct gaatgagatc cctcttaact gtaacggtat ggaccttatt
    42301 gtaacctcca tgcgaaccaa cagtgattac ggcatcccta cgcttaatgg cgccgccctg
    42361 ctcacaggga tcaacgacga cgcgttaaaa caacaaatca aggctctgtt aactcaataa
    42421 aaggaggcat tatgtttgat tacatcctgt ctctcggcgg taccgttttc gtccctatca
    42481 tcatgatcgt gattggcctt atcttccgca tcccctggct gcaggcgatt aaggcagggg
    42541 taacggttgg gattggtttt gtcggtatgg gactggtgat cgtcatggcg atcgacagcc
    42601 tcagtccccc tatcaaagtg atgattgagc gtttcggcct ggccctgcat gtctttgacg
    42661 ttggcgcagg ccctgcctcc ggcgttggtt atgcgacagc cattggcgcc atgattatcc
    42721 cagttatttt tcttctcaat gtcgccatgc tggtcacccg tctgaccaaa acgatgaacg
    42781 tcgatattta caattactgg cactacgcca ttacgggtac ggtggtccag cttatgaccg
    42841 gtagcctgat ttatggtgtg ctgggggcaa tctgccatgc ggcgctatcg ttaaaaatgg
    42901 ccgactggac ggcaaaacgg gtacagaaca tcgtcgggct ggaaggtatt tcgataccgc
    42961 aaggctacgg ttccagttca gtaccgcttt tcgttctact tgatgctatc tatgaaaaaa
    43021 tccctttcat gaagggacgc aatatcgatg ctcaggagat ccagaaacgc tacggcatgg
    43081 tcggcgatcc ggtgattatc ggcgtagtgc tcggtctgat ttttggcctg gcggctggtg
    43141 aaggctttaa aggttgcgcc agtctgatga ttaccgttgc ggcaatcatg gtgctgttcc
    43201 cgcgcatgat ccgcctgatt gttgagggcc tgatgcccat ctccgacggt gcaagaaagt
    43261 tcttccagaa atatttcaaa ggacgtgaag tgtatatcgg tctggatact gctgtgacgc
    43321 tggggcatcc aaccaccatt gctgtcggtc tgctactgat acccattatg ttaattctgg
    43381 ccagcatttt accgggcaac aaagtgttac ccctcgcaga cctgccggta gcaccgttct
    43441 ttatctgtat ggctacggtt atccaccgcg gagacctggt caggacgttg ataagcgggg
    43501 tcatcgtgat gattaccgtc ctgctgatcg ccacccagtt cgcgccttac ttcactgaaa
    43561 tggcgctcaa aggcggtttc agttttgccg gtgaaagtgc gcaaatctca gcgttgtccg
    43621 tgggcaatat gttcggttgg tcgatttctg aattgatgtc gcttgggatt attggcgttg
    43681 tcgtggcggt cggtattgtc gccagcgtcg tgctgttctt acgtaaacgt gaattatcgg
    43741 aataaggagt actttcaatg agttggctga aagaggttat tgggacggaa aaagcggtca
    43801 tcgccatgtg ccatctacgt gcattgcccg gcgacccgag ttttgatgcg cagttgggga
    43861 tgaactgggt tatcgacaaa gcctgggacg atctgatggc gctccaaaat ggtggggtgg
    43921 atgcagtcat gttctccaac gagtttagtc ttccctatct cacgaaggtg cgtccggaaa
    43981 ccactgcggc aatggcacga atcatcgggc aattaatgag cgatatccgc atcccgtttg
    44041 gcgtgaatgt tctgtgggat ccggtcgcct cattcgacct ggcgatggct accggcgcta
    44101 agtttatccg cgagatattt accggtgcct atgccagcga ctttggcgtc tgggacacta
    44161 acgtcggtga gaccatccgt catcaacacc ggattggtgc aggcgaagtg aaaaccttgt
    44221 tcaatatcgt cccagaggcc gccgtgtatc tgggcaatcg cgatatctgt tctattgcta
    44281 aatcgacagt atttaacaac catcctgatg cgctctgtgt ttctggcctg accgccggga
    44341 cgcgtacaga cagcgcgctg ttaaaacggg tcaaagaaac ggtgcctgac accgtcgtgt
    44401 tggccaacac gggcgtctgc ctggaaaacg tggaggaaca actcagtatc gccgatggct
    44461 gtgtgaccgc aaccaccttt aaaaaggacg gcgtttttgc caatttcgtt gaccaggcgc
    44521 gagtcagtca gtttatggag aaagtgcatc atatacgccg ataagatagg gataagcaga
    44581 gcgcttatcc ccggcaaaga gcaataaccc ttctgcgatc cctgccgcct tcgtcaaaag
    44641 tatcgacggg gatcgctacc agattaaccc gaatcggcat ggagtcaacg acggatgatt
    44701 aacgatatta aatgggtaca ggcacaacgt gaagccaccg actggcgtca ggccgtggaa
    44761 atcgccaccc gcccgctggt agcctatggt gccgcgcagc catgctacgt taatgggatc
    44821 atcgaaaata cccttaactg ggggccttat tatctgatag ccccaggaat cgccttgcct
    44881 cacgcccgac cagagcaagg cgcaaactat aatcaggtca gtatcaccac gctacgcacc
    44941 ccggtcgcct ttggtaacga ggagtgcgac cccgtctggc tgcttctctg cgtcagcgcc
    45001 acagatgcca acgcacatat tttgaccatt cagcgcatca gccagtttat tgattcgcca
    45061 caacgcttag cggcagtggg aaatgccagc acagacgatg cactgtttgc gctggtatca
    45121 ggttaacgga ggcgcttatg atcctgcacc cttctctcgc cagcgcaaat cctctgcact
    45181 atggtcgtga gctgacggca ttggacaatc tggattttgg gagcctgcat ctggatattg
    45241 aggacagcag cttcattaac aacattacgt ttgggatgaa aacggtgcag gccgtcgcca
    45301 gacagacgcc tcatcccttg tcctttcatt tcatggttgc ccggccacag cgctggttta
    45361 acgcgctggc tgaaatcaga cccgcgtgga tcttcgttca tgcagaaacg ctggactatc
    45421 cttcggaaac gttaactgag atccgccata ccggcgcacg agccggactg gtatttaatc
    45481 cggcaacccc gatcgacgcc tggcgatatc tggcgtctga gctggacgga gtgatggtga
    45541 tgaccagtga gcccgatggg cagggacagc ggtttattcc ctccatgtgc gagaaaatcc
    45601 aaaaagtacg cacggctttt cctcagaccg agtgctgggc cgacggtgga attacgctgg
    45661 cagctgcaca gcaactggca gcagccgggg cgcaacatat ggtgattggg cgcgccctgt
    45721 tctcttcatc tgactatcgc gcgaccttag ctcaattcgc caccctgtaa gaggagattg
    45781 tttctgatga gccaacagag acctgaccgt atcaaacaga tgctgcacta cctgtggcaa
    45841 catcgtcacc tctccacaca gcaggccatg gagctttttg gctatgcgga agcgactgtc
    45901 cgtcgtgatt tccagtatat tgccaaccag tatccaggca tgatccgcgg tcacggttgc
    45961 ctggacttcg acgacagtac agacgacaaa gagtacgtct tcgacgtgaa acgtacattg
    46021 caatctgtgg cgaaaagaga aatagctgcc cttgcccgaa caatgatcaa agatggcgac
    46081 tgcttttttc tcgactccgg ctcaacctgc ctcgaactgg cgaaatgcct ggccgatgcg
    46141 cgggtaaagg tcatctgcaa cgacattaaa atcgccaatg aactgggctg ttttccccat
    46201 gtggaaagtt acattatcgg aggactgatt cgcccaggtt atttttcggt aggagagagt
    46261 ctggcgctgg agatgatcaa tgccttttcc gtagaacgtg cgtttatctc ctgcgatgcg
    46321 ctgtcgctgg agacggggat caccaatgcc accatgtttg aggtcggggt gaaaacacgg
    46381 attattcaac gctcgcgtga agttattctc atggccgatc actcaaaatt tgatgccgtt
    46441 gagccgcatg ctgtagcaac actatcatgt attaaaacga tcatcagtga cagcgggtta
    46501 cctgagacta ttgcacagcg ttatcagcga gccggatgtc agttgttttt acctcattca
    46561 ataaagtgat aagtaagttc gttcgagagg gatttcaagc aaaaataatc aatggcaccc
    46621 aatagaaaat attggcgatg cgctcgaacg aataaagaag ccctatgcgc aatccacacg
    46681 ctacgatgtt gcaacaacac gccatctact ttttattctc attcactaaa tgtggttgtt
    46741 ctggattcat tattcaaagt gtgtacaaga tcacatttaa tcacttcatt acagttcagc
    46801 atgctgaaca aagcatattt tccactatgt aatgccgata ccatttattc catgagcaag
    46861 aaggagccat tatggttcgc aaagggtgta attcattagt ccgtgctgaa aaaatattaa
    46921 cccacattgc atgggttggt atggcaagtt atatggagtt attaaataaa ttccagtacc
    46981 ccaaaagcag cctgcttaat ttattgaatg taatggtgga gtgtggtttc ttaattaaaa
    47041 acaaaaacgg ctactattca ctgggaataa aaaactacga attgggttgt caggcgttac
    47101 accgtcaaaa tatatttgag gtgactaagc ggccaatgca ggagttgtcg ctgaaaagtg
    47161 gactggtttg tcatcttggg gcgatggaga gcatttcggc tatctatctc gacaagatcg
    47221 aaagtccgga ctctgtaccg acgagcaaaa gctggattgg caaaaaactt gagttgcata
    47281 ttaccgcgtt gggtaaggca ttattagcct ggaagacacg agaagaactg gattattttt
    47341 tagaagcact cacgttaact ccacatacgc gcaatacatt taccgataaa aaattgtttc
    47401 tggaagaatt acaaaaaaca cgccttcggg gatgggccat agacaacgaa gaatcaacct
    47461 atggggcggt atgtttaagt atgccggtat tcaatatgta taacagagtt aactatgcaa
    47521 tctcactttc tggcgatccg gtagtttatt caggaaataa gatagacagc tatctggaat
    47581 tgctccggaa atgcgctgag caaatatcat atgggctggg atacagaaac gaaaatgagc
    47641 atttacgaaa aggaaactga ggtaatgaaa aaattcagcg gcattattcc accggtatcc
    47701 agcacgtttc atcgtgacgg aacccttgat aaaaaggcaa tgcgcgaagt tgccgacttc
    47761 ctgattaata aaggggtcga cgggctgttt tatctgggta ccggtggtga atttagccaa
    47821 atgaatacag cccagcgcat ggcgctcgcc gaagaagctg taaccattgt cgacgggcga
    47881 gtgccggtat tgattggcgt cggttcccct tccactgacg aagcggtcaa actggcgcag
    47941 catgcgcaag cctacggcgc tgatggtatc gtcgccatca acccctacta ctggaaagtc
    48001 gcaccacgaa atcttgacga ctattaccag cagatcgccc gtagcgtcac cctaccggtg
    48061 atcctgtaca actttccgga tctgacgggt caggacttaa ccccggaaac cgtgacgcgt
    48121 ctggctctgc aaaacgagaa tatcgttggc atcaaagaca ccatcgacag cgttggtcac
    48181 ttgcgtacga tgatcaacac agttaagtcg gtacgcccgt cgttttcggt attctgcggt
    48241 tacgatgatc atttgctgaa tacgatgctg ctgggcggcg acggtgcgat aaccgccagc
    48301 gctaactttg ctccggaact ctccgtcggc atctaccgcg cctggcgtga aggcgatctg
    48361 gcgaccgctg cgacgctgaa taaaaaacta ctacaactgc ccgctattta cgccctcgaa
    48421 acaccgtttg tctcactgat caaatacagc atgcagtgtg tcgggctgcc tgtagagaca
    48481 tattgcttac caccgattct tgaagcatca gaagaagcaa aagataaagt ccacgtgctg
    48541 cttaccgcgc agggcatttt accagtctga ggagaaactc atgtctgttc gcaatatttt
    48601 tgctgacgag agccacgata tttacaccgt cagaacgcac gccgatggcc cggacggcga
    48661 actcccatta accgcagaga tgcttatcaa ccgcccgagc ggggatctgt tcggtatgac
    48721 catgaatgcc ggaatgggtt ggtctccgga cgagctggat cgggacggta ttttactgct
    48781 cagtacactc ggtggcttac gcggcgcaga cggtaaaccc gtggcgctgg cgctgcacca
    48841 ggggcattac gaactggaca tccagataaa agcggcggcc gaggttatta aagccaacca
    48901 tgccctgccc tatgccgtgt acgtctccga tccttgtgac gggcgtactc agggtacaac
    48961 ggggatgttt gattcgctac cataccgaaa tgacgcatcg atggtaatgc gccgccttat
    49021 tcgctctctg cccgacgcga aagcagttat tggtgtggcg agttgcgata aggggcttcc
    49081 ggccaccatg atggcactcg ccgcgcagca caacatcgca accgtgctgg tccccggcgg
    49141 cgcgacgctg cccgcaaagg atggagaaga caacggcaag gtgcaaacca ttggcgcacg
    49201 cttcgccaat ggcgaattat ctctacagga cgcacgccgt gcgggctgta aagcctgtgc
    49261 ctcttccggc ggcggctgtc aatttttggg cactgccggg acatctcagg tggtggccga
    49321 aggattgggg ctggcaatcc cacattcagc ccttgcccct tccggtgagc ctgtgtggcg
    49381 ggagatcgcc agagcttccg cgcgagctgc gctgaacctg agtcaaaaag gcatcaccac
    49441 ccgggaaatt ctcaccgata aagcgataga gaatgcgatg acggtccatg ccgcgttcgg
    49501 tggttcaaca aacctgctgt tacacatccc ggcaattgct caccaggcag gttgccatat
    49561 cccgaccgtt gatgactgga tccgcatcaa caagcgcgtg ccccgactgg tgagcgtact
    49621 gcctaatggc ccggtttatc atccaacggt caatgccttt atggcaggtg gtgtgccgga
    49681 agtcatgttg catctgcgca gcctcggatt gttgcatgaa gacgttatga cggttaccgg
    49741 cagcacgctg aaagaaaacc tcgactggtg ggagcactcc gaacggcgtc agcggttcaa
    49801 gcaactcctg ctcgatcagg aacaaatcaa cgctgacgaa gtgatcatgt ctccgcagca
    49861 agcaaaagcg cgcggattaa cctcaactat caccttcccg gtgggtaata ttgcgccaga
    49921 aggttcggtg atcaaatcca ccgccattga cccctcgatg attgatgagc aaggtatcta
    49981 ttaccataaa ggtgtggcga aggtttatct gtccgagaaa agtgcgattt acgatatcaa
    50041 acatgacaag atcaaggcgg gcgatattct ggtcattatt ggcgttggac cttcaggtac
    50101 agggatggaa gaaacctacc aggttaccag tgccctgaag catctgtcat acggtaagca
    50161 tgtttcgtta atcaccgatg cacgtttctc gggcgtttct actggcgcgt gcatcggcca
    50221 tgtggggcca gaagcgctgg ccggaggccc catcggtaaa ttacgcaccg gggatgtaat
    50281 tgaaattaaa attgattgtc gcgagcttca cggcgaagtc aatttcctcg gaacccgtag
    50341 cgatgaacaa ttaccttcac aggaggaggc aactgcaata ttaaatgcca gacccagcca
    50401 tcaggattta cttcccgatc ctgaattgcc agatgatacc cagctatggg caatgcttca
    50461 ggccgtgagt ggcgggacat ggaccggttg tatttatgat gtaaacaaaa ttggcgcggc
    50521 tttgcgcgat tttatgaata aaaactgaaa gcataaataa aaacatccag acacctgaat
    50581 tatccgactc tgcatcattc gttatttgct cagtcggata atgatgggtc gctaccctgt
    50641 tatgagagga cgttatgcca ctaattatcg ttgtggcagg gattgcttta ctcctgcttt
    50701 taaccataaa aattaagctt aatacgtttg tttcgttaat tattgtctcg attgctgtcg
    50761 ccatcgccag tggtatggat ttgagtaaag tcgtcacctc agtcgaatcc gggcttggcg
    50821 gtacgctggg ccatattggt ttgatatttg gcttcggtgt tatgctcggt cgcttgctgg
    50881 ccgatgcggg tggtgcgcag cgaatagcgc tgaccatgct gaattatttc gggaaaaata
    50941 aacttgactg ggcagtggtc tgttcggcgt ttattgtcgg tatcgcactt tttttcgaag
    51001 tcggtttaat tcttctggta cctattttat tcgctattgc ccgtgaagca aaaatatcac
    51061 cgatgtttat gtgtgtgccg atgctttctg gtttgctggt cgcacacggt tttttgccgc
    51121 cgcaccccgg cccgactgtt atcgccaggg aatatggcgc tgatgtagga ttggtactga
    51181 tatacggtat cattgtcggc attccaacct ttattctttg cggcccggta ttgaataaat
    51241 tctgccagcg cattattccg gacgccttta aaaaagaggg gaatatagcc tctctcggcg
    51301 cgacccgccg attcagcgaa agcgaaatgc caggctttgg gatcagtttt ctcacggcaa
    51361 tgctgccggt gatcctgatg gcggttgtca ccatcatcca gatgactcat gccaaaagtg
    51421 ccgcggattc tggcttattt tacaacgtga tactgttctt aggtaattcg acgatcgcca
    51481 tgctgatttc actgctgttc gcgatctata ccatgggcct ggggcgcggg aaaacaatcc
    51541 ctgacctgat ggattcttgc ggaaaagcta tcgcgggtat tgccggtctg ctgctgatta
    51601 tcggtggtgg tggtgcattc aagcaagttc tcatcgactc tggcgttggt cagtatattt
    51661 ccaccctggt ttcgggtatg gatattaacc cgatcttgat ggcctggggc gtggccgcat
    51721 tcctgcgtat ttgtctgggt tcagccaccg tcgcggcgat ctctaccgcc ggtctggtca
    51781 tcccattgtt ggcagtacat cccaatacca acctggcctt gattacgctg gctaccggtg
    51841 ccggatcctg tatttgttct cacgttaacg atgccagttt ctggatgatt aaagactttt
    51901 ttggcctgac caccaaagaa acgctgttat cctggacgtt gatgtccaca ctcttatcca
    51961 tcagtgggct gatttttatt ctgctcgcca gcttagtttt ataatcgggt tcttgccagt
    52021 gagccaacct gttgttcact gtcccccata atttagtgag tgacggcaac cgtctgcacg
    52081 gtgacgaatc gctggcgatc aaaaaggggc gatattgaac cgtcgattat gcttgatggg
    52141 tgtactctca gtaatactgt aaagccggag catgtctccg actttacagt atgggggaac
    52201 agcaggcgat cgctcaaatg gtatgctggt ttatttcatg taaaagaata aaatatctgt
    52261 ataaaatcat gccactggca atctgcatca actctccggg taaacagggt taagatgagc
    52321 atttccccgg tttaatacag gtatacaaaa gtggatagag atcaaacaaa cagcagcctg
    52381 ttcaatgatg atcccgtact tcatgcaaca tggctgtact atcaggaagg caaaagccag
    52441 actgaagttg cagctatcat gggggtgtcc cgcgtcacgg ttgtcaaata cctgcaaaca
    52501 gcccgggaaa atggactggt tcatatcaac ctggatgtga atgtctttgg ctctatcgac
    52561 gctgctttgc aaattcgcga taaattcaat ctccagagag tgatcatcgt gccggatggc
    52621 gaacatgccg gaaaacgtga tgatacaaag ctgatgcgga ctcgcctttc acgtgccggt
    52681 ggcatgtatc tgaatcaggt gattgagaat ggtgatgtgc tgggggttgc ctggggaaga
    52741 acaatccatc agatgagcaa aaccatgacg ccgaagtcat gtaaaaatgt caccgttata
    52801 cagatgctcg gttccatgcc atctcagcca gacctgacga tcattgaatc ctcatcgcaa
    52861 atcgcctaca agctttcagg ccgtgtcgcc tcgctgcacg tgcccgccgt ggtatcaagc
    52921 gccagactgg ccatggagtt acaggcagag cctattattc gatcgaattt tgacgttctg
    52981 actcgctgta cgaaagcctt ttttgtcgtg ggtaacgcgc ttgatgaaaa tcccctgatt
    53041 cgggtgggcg tgctgaataa gaaggaaatg cagacgtatc gggatttagg cgcagtaggt
    53101 gtcatttgtg gtcgcttcta tgacaaggaa gggatgcccg tcgtggctga tgttgaccag
    53161 cgtattctgg gaatcagtct ggctcagcta aggcagattg aacggaagat cttccttgct
    53221 ggtggggaaa gaggctatga cgcaacgttg ggtgccctgc tgggaggata tgtcacggac
    53281 ctgattgttg atgaaggaac ggcagagttt ttgctggcgt gtgaattacc acactaaaag
    53341 aatgaacaac tgccccatgt ggatatgggg caggatttta ttgattagcg gcgataataa
    53401 gaacaatacc cacaatgtat gaaataaaca tggtcagcag cagtttattg gttccggctg
    53461 gcgcactggc aaattctctc cagatgaaga ctccccagat cacggcaatc attgtggccc
    53521 cctgtcccag accgtaggaa atggcatacc ccgcctgacc agacgcaatc agactgaatg
    53581 ccagaccgac acaccaaatc ataccgccca gccagccgca aacatgatcc cgtaagctcc
    53641 cggaaaaata cattttcccg ttgaccggtt ctcctgaaat aggttttttc atgaccagcg
    53701 tgttaaggac aaagttcgac aagaacagcc ccacggcaaa cagaacaagt gctgagtaag
    53761 gcgtcatcag accgctggca ggctggctga aattgtcaga catggagtcc gccaagaagc
    53821 ggaaaaacca tcccatgatg atacccgcca gaatggcagt aatgagacct ttattacggc
    53881 gagatttatc cgcctcctgc gtgacacgac cataggctat cgcggtaaaa ataatggcga
    53941 tcacaacgca ggcgacaccg aggaacagaa tgagcggatc gccctgcgga ttgccgatat
    54001 aggtggtgat cacacccaga gccagtgcca gccccacacc cacaggaaaa gctaccgcca
    54061 taccggcaag attaatagaa gcgaccagaa gaatattgga aatattgaag ataatccctg
    54121 ccagtattgc cgacatcaga ctactgctgc tcgcctgctg aatatcaggt ataaaactgc
    54181 gaccggcttc gcccatagaa cccagcgtga aggcaaataa caaagaacac agcaataatc
    54241 caattgaata atcccaatag aatagaggga actcccattt tttattgctg acaagtttag
    54301 ttgtattcgc ccatgaaccc cagcaaatca tggtaataaa acacattatt atggcgacag
    54361 catacgattc cacgataaac ataaataact ccttgtggta gttataatca atcgttgtgg
    54421 acagagggaa tagcagccat tctgcgaatg gctgtcatat gttattaatt aaaataaagc
    54481 tgtaattacg ctttcaaacc atacgttttg aatttttcaa ggacgatggt catttcggct
    54541 tcagacagca cgggaacatc gtgatgtaat ccgccacatt tgatgtataa atcagccagc
    54601 gtctcggttt cacccgccag ccacaacgtc ttttccagcg tgacgtccat ggccagcata
    54661 ccgtggtgct gcatcagcaa ggacttactt tctctgattc ctttagatac accgtcggcc
    54721 agttcaggac tgccaaaggt ataataggga atacagggaa tatgatccgt ccccgatacc
    54781 gccaccatat aatgaatggc cggaattgaa tgattatgta ttgccaccgc cgtggaattc
    54841 accgcatgcg tatgtatgac agcattcaat tctggtctgg ctttataaca cgccagatga
    54901 aataaccatt cacttgatgg tattttaccc gcttcaggta ttcccttatc gtctacaaag
    54961 acaatcatgt ccggcgtcat ctttgaataa gcgatcccac tcggcgtaat caacatgcca
    55021 ccggtatacc tggcactcac attacctgat gttccttgat taagacctga cgcattcatt
    55081 tcccgacaag tggaaatgat tttttctgct aacttaatcc gttccattcc tgatccttaa
    55141 tagcaatggc cgccgtttta atgctgttcc ccctggagtg ctttactctt caggtcagca
    55201 aacatatccg ccagcgctga cttactggca gcacagactc cccgctcagt aatgaagccg
    55261 gtaatgtatc gggcaggcgt gacgtcgaaa gcgtagttcc cacaacgggt tccttcaggg
    55321 gcggtattga cccagctcaa ttctccctga gggtttatcc catagacatg ggactgctct
    55381 tttccgtcac gttgctcaat cggaatgctt tttccgtcct caatagtcca gtcaatagtg
    55441 ggagaaggta acgcgacgta gaagggaaca tggttatcat gggccgccag cgccttaaga
    55501 taggtaccaa ttttattaca gacatcccct ctggccgtgg ttcgatcggt cccaacaatg
    55561 cagagatcaa cgtcaccgtg ttgcatcagg tggccacccg cgttgtcggc gatcagggtg
    55621 tgaggaatac cgtgtgagcc cagttcaaat gccgtgagtc cgccctgatt acgtggccgc
    55681 gtttcatcca cccagacgtg aacagggatt ccgttttcat gcgctttgta gatgggagaa
    55741 agcgcggttc cccagtctac agtcgccagc cagcctgcgt tacaatgtgt caggatattg
    55801 accacactcc cggccggttt tttctgggct atttcgcgga tgacttctgt accatgtaat
    55861 ccaatttgtt cacacaacgc gacatcttcc tcggcaattt ctgccgccag cgcccaggcc
    55921 acatccttgc gctctgatac acagagatcc ttcagtgtgt ctatcatcct gtcgagggcc
    55981 catttgagat taattgccgt tggtcgggtt ttgataagaa ggtcatagta acgctgcaga
    56041 cccatgtcac tggcatcgtg attcattccc agcgcgatgc cataggctgc aacgacacca
    56101 ataagcggag cgccacgaac ccacatctcc tgaatagccg tagcggccat ttccgcagag
    56161 gtaagcgcca ccacctcgaa cttaaaaggc aacttagtct gatcgatgat ttctaccgct
    56221 tttccatccc cggaaaccca gaccgtacga tagtgtttac ctttaatatt cataatgacc
    56281 tcttacttaa ccttcgcaga ctggatgacg gaatccatat ttttaaactg gcgggcattg
    56341 acgataagat cgcgcgccat ggtcaatgca cgtcgttcac atcctgcgcg aagctctgta
    56401 ttttcaatct gtttcagctc ggcaacgcca gcaaaaccaa tgatccggcg attcatttcc
    56461 atgccggcat tcaccaggga atcttcaagc agcgtggcaa aaaactgatc ctgtgcgtcc
    56521 tcaagagcgg ccctgttctg ttgatacatt tctgtcggcc acgcgtcgcc ctgtgttttg
    56581 ttgtcccaga gctggcggaa ctcccgggtg aaaacggacc aggtttggac aatctgctga
    56641 agcagccatt cctgatagtc agcgcgacgt tgctcattcg catcccaccc agggcgtgag
    56701 aagtacgcca gcaggaggtt gccaatatag ttgccgatat caaacgccat tggccccatg
    56761 aaaccgaact ccggatcaat cactttggta tccgtgtcgg tcaccatgat tgagcctgaa
    56821 tgaaggtcgc catgtaacaa tgcctgcgct tctgtcataa atttgtactt ataacgcatg
    56881 gcaacctgga tcatctctac atcagcccag gccttatgga cggcatcgtc cagctcagga
    56941 gaagtccagt tattccgttc agcgttgtaa tagggctctg tgaagatcag atcttccgta
    57001 attttgcaca gctcatgatt caacgcgaac tcggcagtaa gcgctttttt ctgttctgct
    57061 gacatgccaa tgtcagacgt attgaagaga gtctgtgcta aaaatctgcc gatatcttca
    57121 gctagtttag ggaatttttt accgttaatc agttccttac gcagaatcac gtgaggagtc
    57181 agatactcca tggcaaacag cgccatttcc gggtcgtaaa aatagacctg cggaatcagt
    57241 gtgtgacccg cgtacttggc ttcctgacat aagacgttgt actcaaagcc cgcacggctc
    57301 agagataaag gccatccttc gccccctgca cgaagccagg gcagagcttg ttttacaatg
    57361 atggttttat gtgtcccccc aacgataaac accatgttca ggtttccgtc ccccacttct
    57421 gaaactttcc actcttcagg taaccctccc ggttcgcatg aaggggtcag tctggacgag
    57481 agataacccg gcagcgtgtc acatgtcagc ggcttgtaac ctgatggaat tgaatccgtc
    57541 ataacagttc tccttgtcta cacagaatga taatcaactt gtcatgtgac aactatacat
    57601 tatcaaatgt aaaattattt gtgacacatg tagatttttt gaactgtata atattaaaaa
    57661 tgatgtcgta tggagtcatg atctttacag gaatcagcag aggaaaacat ctattattac
    57721 catgtaattc atgttgttat gtcgcaacaa tagtactgca cggggaatta ttacgggcaa
    57781 acttaggcga aaggaagtac tgaagtgaca gaataaatca cactttttgt acatatgtac
    57841 ttttctattg tcttttgttg tggaaacaaa tagtgtttgt catgccgatg ctaatagaca
    57901 gatagattcg agggccaaca gccccatcat catgatggag caacagaggt gagtctaacg
    57961 tttccgcact tcttatcgat cggcactaac gatcaatatc gataaactga tctatcaaaa
    58021 cgattatctt tcttgcaacc tgcctggtta tccttctctt acattgctcg cagaaaagcc
    58081 cttttattac ggttagttat ctagctgaga cttgctacgc ccattttcca tgtaccccaa
    58141 atcacgaggt cacaaaggcg cggaaagatg aagagatgtg cacgttgcaa caaaaaaaga
    58201 agcgtccttg aacagaggct ctcactcgat aaagaattct gcgacgagtg cttattcgat
    58261 atcgatgagg aaatcaggga aagaaggatt atcacgatca gacgcctcaa agatgacaac
    58321 gaaagaaaat cgtagctcaa tatgaaaaat ggaatcgatg tcagcgtcat tatccctgct
    58381 tacaacgccg ctgaaaccag cgttagtttg gttactaaaa tcctcagtga accccacttg
    58441 gctattgaac ttatcattgt cgatgacggt tctactgatg aaactggcaa cgttcttcgt
    58501 caaatccacg atgatcggct cattctcatt gagcaggcca atcaaggtgt ctatgccgcc
    58561 cgaaatgctg ctctagcctt gcaccaagga gaataggtta tattccttga tgcggacgac
    58621 gatatagcag atgggttcat agaaaaaaga tggcaggcag ttagtacgtc gcaagccgac
    58681 gtcatgatct ttaatggctg gcattcctgt gctgaccaac atcgaagagc ggttcacaaa
    58741 aagcagccct atggtacgcc tctgtcaggt tattaatgga ttagacactg tgttacacac
    58801 cgcgaatggc cgcattatct gtggctgcag atggtcaggc catcctatgt caggcagcat
    58861 tcactgcgct tccagtccgg caggagccat aaagacattt tttaaacggt tcatcttgca
    58921 gcgagcaatg gttgattctg tacattggat ataaaggact atatctatat caacaataag
    58981 gcctctatca cacatcggcc ggattactac gttgttcgtg ctataaacta tgttgaagtg
    59041 atcgcgaaaa tcatccaagt agcggaacaa gaacaaaaca aaaaataaag gtatttttgt
    59101 accggcatgc gctggtagag gcacgtcatt ttttgggact ctaccgcaat cgcgttgggg
    59161 ataaatcggg catgaaggac tgcttcaaag cgaacatatt cgcagcccat ttattcagag
    59221 gtatcagcag ttttagcgat gtgtttttct ttattaagct gctgggcaag ctattctgat
    59281 aagttgtgcg ttgctacacg acaccagcgc acagcttaat ttcacgcttt ctgactaaat
    59341 tgttcaggcc ttttctatag gtgcaaacac aactccccgt actttcctct tccaactcaa
    59401 aatcatcgca ccttgctgac ccaagaaggg agcataccct gcactagtat ccgcacagct
    59461 aatactctat tgataccaac aacgcttcag cacaacaaag taactcgata atcacaactc
    59521 aaagtgaaga tgccaaaaac tcgtttgaga gcaataaatt caataacatg aaaaaaatgt
    59581 attttgcatt gaaaatctta cttattgcgc ttggctacaa ttgcatgctt ggtaatatag
    59641 attataaaac accagactag ctgcctggta agtaatgtaa tagcagagga aatatcacga
    59701 tggatcttgt agatagtgtc gaagcaggta agctgacgat cagtgaattg attgatgccc
    59761 tggcgaaaga taaaaattac tcagcttcca agtgggatca gcgataccgt gaatttacga
    59821 ccttgctaca acaaacctca actttttctg aacctgaaac ggatggttta gtcaagaggc
    59881 tctggtatga acgcgataac ggtattgcca gtattcgcca gggcgttcca tccttagcag
    59941 aatatcagca aagcctgcca ctgcttagag aactaactga acgaattcgg caacagccgg
    60001 atgaagaaac ttaccaatat gttggcaatg cactacaaca agctaaagaa aacggacttc
    60061 tcaagcgtat gtataggagt ttgagaaatc gcgtctttgc cgcgttctcg ccagaaaact
    60121 acaccagtac tgtggatgag aatgctttta gtaaagcagc agaattccta aatcagcact
    60181 tccatctcgg tctggcactg accggaaatt ggttacagaa aaactatgaa ttgaaacaag
    60241 ccatacacgc ccaatctcct gacacagatc cttattacgt gaatatggcc atctggcatc
    60301 tctatgaatt gctccgtgaa cgcgataatg aacaaaagca ggagaaaata gcgagcacta
    60361 cattcataac cagcaatgag cccatcgaga acaagatcat cctacattca ccaactaacg
    60421 tgatcttctt tggcccccct ggcactggca aaaccttcag gttgcagcaa aaaatgaaag
    60481 agtacacttc tcatgctgtt ccagctgatc gtgatgcctg gctggattct cgccttgaat
    60541 cgttgaattg gatgcaggtt ataacgctgg tgctgctcga tcttgggaaa cgagccaaag
    60601 ttcgccaaat tattgaacat atgtggtttc aacgtaaggc attattaaac ggtcgtaatg
    60661 gcaacctatc gaatacagcc tgggcagctt tgcaatccta tacagttccc gagtcgttaa
    60721 ccgttgatta taagaatcgg cgtgagcctg ccgtatttaa caaaacagat aacagcgaat
    60781 ggttgctagt tgattcacag ctcgagcaag tggaggattt ggtagagctc tacgccgaac
    60841 ttaaacgagg ccctaaatct gccgaagcca tccagcgttt tgcggtggtt acgttccacc
    60901 aatcttacgg ctatgaagaa tttattgaag gtatacgcgc tcgctctgac gagagtggca
    60961 atatctctta tcccattgag ccgggtatct ttatgcgcct ttcccaacgt gccaatgccg
    61021 atccaggaca tcgctacgcc attttcattg atgagatcaa tcgcggtaac atatccaaga
    61081 tctttggtga actaatctca ctcattgaag tagacaagcg tgcaggcatg cccaatgcaa
    61141 tgagcctaca actggcttat agcggtgatc acttcagcgt acccgccaat gtcgatatca
    61201 tcggagccat gaatacagcg gaccgttctt tagctctgat ggacacggct ttgcgccgtc
    61261 gctttgactt tgtcgaaatg atgcccgatc tctctttact gagtggagct aaggtgaaag
    61321 gcatagagct cgagtcgttg ttagagaaac tcaatagccg catcgaggct ctttacgatc
    61381 gtgaacatac gctggggcat gcgttcttta tgccggtaaa aaatgcactc gatgccggtg
    61441 atgaagaagc tgcgtttaaa caattgaaga tcgcattcca gaaaaagatc attccgcttt
    61501 tacaggaata ctttttcgat gactggaaca agatccggtt ggtgctggca gacaatcaaa
    61561 agcaagacga caacctgcaa ttcgtgattg agaaaaccga cgatctcgat acgctttttg
    61621 gtaacaacca tggtttacga caccatgatc agcaatcaac agcttacgaa ctcaaagatt
    61681 tcgatcaaga gatctggaat attccacagg cttatcgttc aatttatcag ccccagcaga
    61741 ctccccttga tgagcaggct gtaaatcatg ggtgaagtta tctccgtttt tgaatatgac
    61801 ctattgggga gtgacaaagc ggcttcagtt ggcgcaaaac tggtgcctcc actggtcttt
    61861 aattatttag aagcgcttag cttggcaagt aatcaaggga gccagttcct caaactcacc
    61921 tctcgctcag gcttcaaatt gcttcaggtt caaaactacg cggggatgct ttcaacacct
    61981 cacggtttcc agcttgagat actgcccaag gttggtaaaa acctcacagc ggctaatgca
    62041 cgtcaaacat tactgacgat gctaagccat ttgcctgggt ttcgacatat tgaaactcag
    62101 caagccactc ttcaggctca gcgtatgcct ttgcttgaga tttttattca ccagtttttg
    62161 catagcgtta gccaattact taaacaaggt ttacgctccg actatgtgag cgagcaaggt
    62221 aatctggctt ttatgaaggg caagctgatg ctttctgcac agctgcgaca taacgcggtg
    62281 aatcgccata agttttgtgt cgattatgat gactacatgc ctgattgtgc agccaatcgg
    62341 ctcttacact ccgcgctaga taagcttctt agtctaaagc tgtcatcaga gaatcaacgc
    62401 tggctttacg aactgcgctt tgcgtttgac ggtattccac ttagccggga tattgaacgt
    62461 gatataaaca acttacgcct cgagcgtggt atggctcatt acaacgagcc aatggcgtgg
    62521 gcgcaattga tcctgagagg aatgagtccg agtgctttgc agggaaacac caaagcgata
    62581 tcacttttgt tccctatgga agcggtattt gaatcctttg tggcacagac cctaccctac
    62641 gaattacctt ctcatctcaa agtaatacca caagcggcta cttattctct tgtgaagcac
    62701 gggtctaacg attgctttaa acttcggccg gatttgttga ttcagtcacg tcgaccggta
    62761 caaaccaaat tggtgatgga cacaaaatgg aagctaataa atagcaactc ccaaaaaaca
    62821 acacagtatg ggctagcgca agccgacttc tatcaaatgt ttgcctacgg tcaaaaatac
    62881 ctaaatggga ttggcgaaat gtacttaatc tatcctgcgc atgatcaatt taaccaaccg
    62941 atacagcagc attttgcttt ctctaaaaca ttaaaactat gggttgttcc gtaccagata
    63001 acagcaaacc gcggggagag gatgatgtgg gtgaaggatg tgtccagtat ataaatttct
    63061 agccaagctg gcgatgaacc agtcctctaa aaaatgggta taatcatagt ttatttacta
    63121 attaaattaa tataaagtaa ttactttatt atatggagaa tttatgaaaa tgggaaaaat
    63181 tgcagttact cctaataatg ataaagcagc cagccactct aaagatgaaa atttagccac
    63241 aaatacaatt aagaaaaacg taaaacctaa atatggcgat gcggcatttt tagagtacac
    63301 tcgactaata gttaaccacc cgaattattt tggaatgcca gatccctttg gggaaaaagg
    63361 tgagattcaa tgggaagctc catcaaatag ggcatcgggg aaatttaaac acactcatca
    63421 acggcgctat gaatggtgga aaaataaagc cagaagtatt gggatagacc ctgacactga
    63481 aaaagcatgg attagtaaaa cagctaaact aattcatcca cttggagtta aaccatgcaa
    63541 gaaatgcggt aaagaaatgg aattatctta ctcctaccca aatgagcatt tcttttctcg
    63601 ggttaggaaa cttaattata tagatgagac ttttgagcta tctcaaaatg aacacatagt
    63661 tgatttgttg actcgtttag atgatagatt tggtgaaaga atatatttag atttaccaca
    63721 tttattttct acaaaatcca tcacgattcc agatatttca agcaatctcg aagcatggat
    63781 tgagtatcta aaagaacaat acatacctca agaatcacgt atgcttagcc ctggagccat
    63841 ggcaaatcct ccagatcgtt ttgatggatt tcattcattt aaccgctgtt gtcgctctat
    63901 tgctgataaa ggaaggacca aagaaaatct taaatcttat gttactgaca gaagagtttt
    63961 cgaatattgg gtggatggtg attgggtagc tgcagacagg ctaatgggcc aagttagaac
    64021 aaataatata tttattaacg aggaatgttt gaatgccgga aatggtggtt tacatccaac
    64081 tccttgccaa gcagatcaca ttggcccaat ctcactaggg ttttcccatc gcccacaatt
    64141 tcaactactt tgcaaatcat gtaatagtgc caaaaacaat cgtatgtatt taagtgatat
    64201 aatctcttta ttagaagctg agaatgaagg tcatacggtt atttcatggt tcgcagaaga
    64261 agtatggaat cgactaaaac actctgtaga tgatagtgaa aaagccttaa gactaagtaa
    64321 aatactacgt gataatagac atacatatat gaatcttctt aaaaaaataa tggatgaagg
    64381 ttattatact ttccttgcaa gtcttttaca tcttgaggta gccaattata acccaatatt
    64441 tgagggatta tgcatttcaa atcatttaac tcattataaa tcattaaaaa aaataaaacg
    64501 tgaatcaaaa tatgctgctg tgcaaaaaac aagacgaatc agaatagcat ttacttcatt
    64561 gaatgattat catagaaaag aaaatcggaa cgcatttatt gtatcaaatg aattatctga
    64621 gaagtttttc tctgaagcta tggataattt aaaatcatta agtgaaatta catcttgctt
    64681 agatgaaaaa atatccggaa taatatctga gaattcagat tcaaaaaatg aatttcgaac
    64741 aatcattaca gatctacgag aaatagtgac aaacaataaa gaaaaattca acttgatatt
    64801 aaaatatcta atatcaggga tgagcgaaat aggaaaagaa ctggaatcat attgggaaaa
    64861 tgatcgttat gttcgctcaa tcccagaaga atttatagag taagacatag ccctcttata
    64921 aagagggctt tatattcctt tcataatatt ctgaaggagt aatattacct ctcatgaata
    64981 attgatagtc attttcgggg tgatggatat aatatttacc acttccatct tcaccatgtt
    65041 gcaaaggagg cagatcagat aatgcatcct ttacagatac ccaagattcc ttatcttctg
    65101 ttaatttttg tggaggttca attgaaaata aagggtcgtt tgaaccaacc aatataaccc
    65161 tttttcggcg ctgagggatt gcataatgtt cagagtttaa tatccatcca ttcatagttt
    65221 tcatggttga tgagaattct tctttgactc tttcaaagac cttacctttt tccatattca
    65281 acaaaccagc aacattttca aaaataaaac cgtttggttt tatctcattt aagagagatt
    65341 tataatgcat aaaaagtgaa tttcttggat catccattgt tctggcattt cctgccgtag
    65401 aaaatccctg gcatgggggt ccaccaagta tccataattg tttatcaaaa tataatttct
    65461 taaatcctga tattttatct gctatttttg aaaaagtttc attatcagaa atgctgccac
    65521 ataaaacttc tttgtgaaca ttgtttgaat atgtttgtag atatttttct tcaatatcat
    65581 tcgccaataa tggagtccat ccagcccatt tgaatcctag tcccagccct ccagctccgg
    65641 aaaataaatc gatataataa ccagtattac ctatcgcctt ctctatttct ttcgcaatca
    65701 gaaaagctaa aaatgggggc actgcattac cgatttgggt attaattgct gtttgacctc
    65761 cgaagaaaat aaaatcatcc gggaacgatt gtagtcttgc agcttctctc tgggatagaa
    65821 cacgatcttg ctcatagtga atatggcaac cattcccagg tcggttaaaa tatgtattga
    65881 tagtatatgc aggcatatct ggaagtaatc taccgtagta tgttgaacgg cttccttttc
    65941 cttgtgcata actatcccta atttgctcta ttcttttaga cggtacatct aaaggaatat
    66001 ttttccagtt gccacctggc ggtactgagt taacaatcat catatccaat tcacttagct
    66061 ttgctgcctt gtgattggga atcataaatg tttcactttt agtcataatg ttgaaaaatc
    66121 ctcaataaac tttctttttc agctaactca atcttatcaa atgaagacag aagcttatta
    66181 aattcattca aatccaattt gtaaatattt ttggcaacat aagcctcaat tttcaactca
    66241 gcatcacaac atccctctat gcaagaaatc acaagttgtt ttaattcaga atgttgtaat
    66301 aatatttttt cagacggtat tgcagttttt cgtaaggctg acaatgatac atgaccagta
    66361 gctaaaaagc ttcttaattg aaattcaaaa caaagggagt ttataatacc aagcaaacta
    66421 aagagcgaat cttgcgaacc acttttataa tagaccaccc ctaatgaatt ccctgccaaa
    66481 gagttaggag gtattatcgt tgcaatcatt cgcctttttt gactcgggcg tgaaatgtca
    66541 cgccatgcaa tccttacgaa attagaattg gatagaacct tatcaatttt tttcgttatt
    66601 cttaagctct tctcattatg aaaattatac ctcccaatca tttttccttt aatgaataat
    66661 aatccgccgc cacttttttg tgtccagtca ctactaccag tttcatctat ttctcttcct
    66721 gcccatatct ctccattttt ttccagacta ccccatgttg ggtatccctc ttgcagcttt
    66781 cttaacaaat gaacggcatg aacaccttga gataccggaa tgctaagata ttgagaatct
    66841 atactagaca aatcaaaaaa acctagtgat ttaatattta agtttttatc atacacacta
    66901 agttgaatat tatcattaag ggattcacct ttattaaaaa taaaagaaca tgaagaaaca
    66961 tcagcaccgc cgaataattt agcctcagct ggataataat ttatgcaatc aatcctccct
    67021 gaattaaaaa acttttcacg taatatgtac gattgttcat ctgcgaaaaa agatgcaggc
    67081 ataactatag ctaccaatcc atttttatta catatttcta agctaagttc tgcaccaaca
    67141 cgtgaaagat tagtccccca cccagcaaat ttacgcttcg gctgagaata tggcaatact
    67201 cttgacaaat aatcatcaaa ctcacgaaga gagtctatat acatcgattt catagatggt
    67261 tcaaaaaaat cgagctctct agaatctggt tttatattct cccaaggcgg attggttata
    67321 acacaatcaa aataatcaac atatttttta aattcataaa aaacatcact attttttata
    67381 gtaatgttaa tatttgcacc tgctaactta agttcagaca acgcattttt agcataagtg
    67441 aggcctgtat tttctatatc gaacagataa acattccact ctacatctgg aaaaccattt
    67501 atcatccatt gtttaataag caatgtaata agtcgaccat cccctgaaaa tggatctgcg
    67561 acattataac tttttttatc acaggaattt atcaatggta ataacttctc tatcatcaaa
    67621 agagctattt ttggatctgt atagtattta cctgtcgctt ttttagtgaa ttttgttgtc
    67681 tcgtccaagg acagtgaatt aattaaatgg atatgtttca tgtaaaatat ctcttccatt
    67741 aataatatgg attatatcat ttaacttgtg aacactcaac aataactgta cataaaaaca
    67801 gtcattaaag atttccccta gagtgagcat cgctataaaa atttaaatta attttatcct
    67861 gctccgaaag aaacagttat catttattcc ataaagaata acttgcttta taatcacttt
    67921 aaatatcgat atcaatttta atactatggt tacttaccat atcaataaat agcttaaccc
    67981 gttatctcga taccccttaa gaaccccttc cgcaactgca acataatcta cgctcatcca
    68041 catgcctggt gcgtattacc gtgaaggaga tcggtgagta acatcgatgg agatcggttc
    68101 gtgtcacttt cacagaaccg tttttaaatt accttcactg atctccttcg gtcaacggag
    68161 attgtatttt ccgcattgat tctcctttaa gttcgatctt gatactgcca tgaaccagtc
    68221 gatctaggat ggcatccgca tgtgtggagt ctccgatcat tttgtaccag ttttccaccg
    68281 gcaactggct cactacgatg attgagcctc gttggtacat cagatccact atttccagca
    68341 ggtcgctacg ctgttctgat gagagaggtt ccagccccca gtcatccaga agcagcagat
    68401 cgctattatt cagcctggtc aaaagtttgc tgtatcttcc atcagcatgc ccctgatagc
    68461 actgttccat cagcgcttta aggcgataat agtagacctt gtatccctgt cggcaggcat
    68521 tatgaccaag tgcacatgcc aggaacgttt taccgctgcc ggtggccccg gtcagtaaaa
    68581 tattttgttt cagggttaac cagtttccct gactgagtga acggatgagg gccctgtcca
    68641 gccctctatt gttacgataa tccagctttg ataactcagc attaagtctg aaccgtgcat
    68701 gtttgatcag acgctctgcc ttcctgtttt cacggcaggt tagttcttct gctgtcagta
    68761 atgacaggcg ttcttcgaag cccagctcct ggtatgtgcc cggttgagca agttgctttt
    68821 taagcgcatc acggaagccg gtgagtttaa gtgcggtcag ttgttcgtaa agatgattca
    68881 tcattggatc ccgtatcagt ggtaatactc actgccgcgt acgttttcgt gttccatcgt
    68941 ggataacaga tctggttttg gatcctgaac aggttgttta tccagacctt tttccaggat
    69001 cgatttaata cctgacagac gccatacctt tgttttcaga gctcttgcac atgctgcatt
    69061 aagtctggct ttactgtatt ttttatgaag gttcaggagt ccaaggcaga agcgatagct
    69121 ttgttccgga tgtggacgag agttcagtat atgaagcaca taactatgag tttcactgcc
    69181 tatgtgcccc gcccattcca gaagacgctc tggcgtccag gtggcatgct gtcgatgagc
    69241 ttcaggcatg tgctcgttgc gggtaagtgg tggcacggga gtaatgagtt tggggagggt
    69301 accagaatgc caggctggtg cggtatgctg acagcatcct gttaaccgca gcataaagta
    69361 atgagaggct aatggaagtt aaaaccacag cagttccgtc gttgttcatc ccgggagacg
    69421 ctggaaaaag tgatttccca cacgcgttat aaacttaccc ctgcggagct ggaagccttt
    69481 aactctgccg ttgacaaccg gctggcagaa ctgacaatga acaaacttta cgatcgcgtg
    69541 ccggcttccg tctggaaata tgtcacctga atcttgaatc cgcgctacga tgactccggt
    69601 gttatctgtg caccggaaga tcgggaacat taagcagtat aacggcaaca aagaattcga
    69661 aatcaaaagg gaatggtgta cagaggggca ggcgttcagg cattaccggc ctgtatcttc
    69721 tgaaggggac tcgtatttgt cggggctgag tattccgctc gattcgatca cggattggcg
    69781 gaatacctga ctgtgattga ttcaggtacc attttgcagg gagccggtgc gttactgtct
    69841 ggcacaacaa acgcagcggg tcgctctgcc ccgccgattc ctgataatcc ctcccctgta
    69901 acctactgca acagtacaaa tacactgacg cctgaaacgg taaccagaat cagcaccagc
    69961 agtaacgtca gtaaacagag cagacgatcg gtcttccggt aataaccgaa ggggtcgaca
    70021 gcgccacaat acgggcaaca aactgtatta tgagtaattg cgttaccaca gtccctgcac
    70081 ttctttttgc gtatcacgat agctccctcg ccacgcctta tccgtaaccg gttttttaca
    70141 ttaaaaaaaa caacgccggg aattcaatcg tcagtccgga gacgaccgtt cgggttatca
    70201 cagagtgcct gagacagtgt cctgccggag gtcacttcag ggactctgcg tattttttac
    70261 gacgtggcta ttccgtatga accatacgga gatttaacca tgacctacaa atacaacccc
    70321 ttctggcagc aacgtattcg tgagacggtg cggcacgcac tgaatgttca tccccgcctg
    70381 acggcattgc gggttgacct gcgtttcccg gatgtaccgg cagcaacgga cgcagctgtg
    70441 atatcccgct tcatcaatgc cctgaaagcc cgaatcgacg cttaccagaa acgtaagcat
    70501 cgggaaggta aacgcgtgca tcccacaacc ctgcattacg tctgggcccg ggagtttggg
    70561 gagtgcaaag gtaaaaaaca ctatcacctg atgctgctgg ttaaccggga tacctggtgt
    70621 cgtgccggtg attaccgtgc tccgggatca ctggccggga tgattaaaca ggcgtggtgc
    70681 agtgccctgg gagtggatgt cgggtgccat gccacgctgg tgcattttcc ggcctggccg
    70741 gcggtgtggc tggaacgcga tgatgacact ggctttcagc aggtgctgga acgtgctgac
    70801 tatctggcga aggaacatac caaagctcac tgcaccggtg agcgcaattt tggctgtagc
    70861 cggagttgag cacgactggc gcgccaatac ccggcaaacc aaaagtatgg gttgttgtct
    70921 ggcggcaacg atgtatccac tcgcccactg actgtttttt acgtcagtgt tccattgtgt
    70981 gacaaccaca gtgctgtaac agcaaccagc ctgctttacc caaactccag tccagatttt
    71041 aactgaaccg ccattcacac cctgaccacg ctgcccgtac cgggacggct gttgcctgcg
    71101 tgtcgtctgg cggctgagga atatgactat gtacgcaaaa tcctttatcg ctcttgatgg
    71161 caacggacgt ctgacgggcg cccgtactgc acaggccgca ccttatgctc actacacctg
    71221 ccacttgtgt ggcagtgcac tcagatacca tccgcaatac gacactgaac ttccctggtt
    71281 tgaacacact gacgacaggc tgacagagca cggtcaacag tgcccttatg tcaggccgga
    71341 gcgcagagaa atacagttga ttaaacgtct gcagcaattc gtaccggatg ccttacccgt
    71401 ggtgcgtaaa gccagctggc actgcagaca atgtcaccac gattattatg gggagcagta
    71461 ctgcacacac tgccagaccg gaggattcag tattccccgg acaactcagg aggaaatatg
    71521 cgaattctga actgttatat ggcgaatgac agcaaaggcc attttgttac ggcgaaagaa
    71581 gctgcgaagc acaaccgaca ggacgttttg tgctgtgtgt cctgtggatg cccgttaaca
    71641 cttcagcggg gcaatgacgg acaaccaccg tggtttgaac atgaccagat gactgtcgct
    71701 gaaaaaatcc tgctgcgatg tacctggctt gacccggcag agaaagaggc ccgtcgtttg
    71761 catctgcagg gcatgacggt tccggattat acggtgaagg tgagaaagtg gttttgtgtg
    71821 atgtgtgacg aagattatga gggggaaaag tgctgcccac gctgcggtac cggggtatac
    71881 agcagggcgt gggggcggca ggaggtgccg tcggaagatg ccagggctga taatccgtta
    71941 cagaggctgt aatggttgcc tccggagcag tttgcggtga tgcctttccc gggaatggat
    72001 tgccagtgcg gggactgtgg agcttaattc cggtgttggt ggcctttcca ttgattttat
    72061 gccaacagcc ccctgtgact gacaggctgc ctcgtcattc cattcgtatc tccagtaaca
    72121 gggggttgta ttgtatttta ttgtaccggt tcaggtgtgc gtttcccggc gcgtctgcac
    72181 cggcttaacc aaattcaaca gggataaaat agtggtaagc gtacagcctg aaccgtctgg
    72241 tcagaatctg acgaattaga caaagtggtg tccaccaaat aagtagtggg aaccaaagtg
    72301 tcagatatgc agaaaaatgt gactcccggc aggcgaaaag gctgccctaa ttatcctccc
    72361 gaatttaaac agctgctcgt tgctgcctcc tgtgaacccg ggatatccat ctcaaaactt
    72421 gctcttgaaa atggcattaa cgccaatctg ttgttcaaat ggcgacaaca atggcgcgag
    72481 ggaaagctgc tattaccttc ttcagagagc ccccagctac ttcctgtgac tctcgatgca
    72541 gctgccgaac agccagaatc gctcgcagag gacccggaaa ccctcagtat cagctgtgag
    72601 gtaacgttcc ggcacgggac gctccgcttc aatggcaatg tcagcgaaaa gctcctgact
    72661 ctgctgatac aggaactgaa gcgatgatcc cgttaccttc cgggaccaaa atttggctgg
    72721 ttgccggtat caccgatatg agaaatggct tcaacggcct ggctgcgaaa gtacaaacgg
    72781 cgctgaaaga cgatcccatg tccggccatg ttttcatttt ccggggccgc agcggcagtc
    72841 aggttaaact gctgtggtcc accggtgacg gactgtgcct cctgaccaaa cggctggagc
    72901 gtgggcgctt cgcctggccg tcagcccgtg atggcaaagt gttccttacg caggcgcagc
    72961 tggcgatgct gctggaaggt atcgactggc gacagcccaa gcggctgctg acctccctga
    73021 ccatgctgta aatctcttta tcctggttgt cacagaataa gcccggtaaa atacgggctt
    73081 atgaacgaca tctcttctga cgacatcttc ctgctaaaac agcgcctggc cgaacaggaa
    73141 gcgctgatcc acgccctgca ggaaaagctg agcaaccggg agcgcgaaat agaccatctg
    73201 caggcgcagc tggataaact ccgccggatg aacttcggca gtcgttccga aaaagtctcc
    73261 cgccgtatcg cacaaatgga agccgatctg aaccggcttc agaaagagag cgatacgctg
    73321 actggtaggg tgtatgaccc ggcagtacag cgtccgttgc gtcagacccg cacccgtaag
    73381 ccgttccctg aatcactacc ccgtgacgaa aagcgactgt tgcctgcggc gccgtgctgc
    73441 ccgaactgcg gcggttcact gagctatctg ggcgaggata ccgccgaaca gctggagttg
    73501 atgcgtagcg ccttccgggt tatccggacg gtacgggaaa aacatgcctg tactcagtgc
    73561 gatgccatcg tgcaggcacc tgcaccttcg cggcccatcg agcggggtat cgccggaccg
    73621 gggctgctgg cccgcgtgct gacctcgaag tatgcagagc acaccccgct gtattgccag
    73681 tcagaaatat acggccggca aggtgtggag ctgagccgtt cactgctgtc gggctgggtg
    73741 gatgcatgct gccggctgct gtctccgctg gaagaggcgc ttcatggcta tgtcatgact
    73801 gacggcaaac tccatgccga tgataccccg gtccaggtac tgctgccggg taataagaag
    73861 acgaagaccg ggcggttgtg ggcgtatgtt cgtgatgacc gcaatgccgg gtcagcgttg
    73921 gcacctgcag tgtggttcgc ttacagcccg gacagaaaag gcatccatcc gcagactcat
    73981 cttgcttgct tcagcggtgt gctgcaagcg gatgcgtacg ccgggttcaa cgagctgtat
    74041 cgcaatggtg ggataacgga agctgcctgc tgggctcatg cccgccgaaa gatccacgat
    74101 gtgcacgtcc gcatcccgtc agcactgacg gaagaagccc tagagcagat cggtcagttg
    74161 tacgccatag aggcggatat aaggggaatg ccggcagagc agcggcttgc tgaacgtcag
    74221 cgaaaaacga aaccgctgtt gaaatccctg gaaagctggt tgcgtgaaaa gatgaagacc
    74281 ctgttcttcg gctctggcca tggtggtgag cggggagcgc tactgtacag cctgatcggg
    74341 acgtgcaaac tgaatgacgt ggatccagaa agctaccttc gccatgtgcc tggcgtcata
    74401 gcagactgac cggtcaaccg ggtcagcgaa ctgcttccgt ggcgcatagc actgccagct
    74461 gaataacaca tccccgtcaa tacggccctc gctgtacgct tacttttgtt cggactgctt
    74521 atggcctttt tccactggtg tagcaggaaa gttaaccggc tgccaccatg agatgacgtg
    74581 taaaccgtgc aggcatgttt tcacgtggtg acgctttcat tgggagtcta cgccggaacc
    74641 ccaatattgt tcacatacat gaatttgaaa aactaagcga tactgctgtc cgtaccttat
    74701 aaacttattt caacgcttcc ggcagcgtca caatccacag ttcactgtca gactccggtt
    74761 tctccagtgg cttcactctg agggtcgttt caacctgctt gtcgtcaagc gtcagctttc
    74821 cttccgcgtt cacccgataa tccttgaatg tcttgccctc gaccggatgt tgcatcgcca
    74881 ccttcacgcc aaagtcgtta tcgttcgcca ccgccagcgt tttgctgtcg atcagcgcca
    74941 acccttcagc cttctcctgc tgccagccca aggagcgcag atcaacaacc tgcgttttct
    75001 gcgcaagggt aatgccgcgc tgtgacaggg ttttctcatc atcaaactcc ggatactcac
    75061 ccggcttgtc gaagcctgac aggtcgctcg ccttattgag atcgacctcg taaatcaggt
    75121 tacgcattct gttgttttta tctcttccct gttcgatcag caggatgtgc tggttatcga
    75181 gcgccacgat gtctccgatt ttagcgtcgc tgtttttgct gtaggccgcg ctgtcgatag
    75241 gataaccgta catcgctgtt tttccgcttg ccggatcgaa gctcaccaga cgcgtaaaca
    75301 gcgccttttt cttgctctta gcgtcgatat ccagcgtact ttgcacggcg acaataatac
    75361 gcccgtccgg catacgggtg aggccttcaa agccgcggtt cgcctgacgc catttgagga
    75421 tatttggcaa accgcccgca atcgcttttt ccccttccgc cgcctgcgga ccgtggattg
    75481 ccaggatttt ccctttactg tcgatgttaa tcaggaacgg gccatactca tcgcacagcc
    75541 agtaacctcc tttaccatcc ggagtgatcc cttccgtatc cagcccgcga ttatccccct
    75601 tcagtctatg taacgtgtcg ctgaaggcca cttcattggt ggagccaatc acgtcgcttg
    75661 ccagcggcag gccgttgatc gcgcctttat cgtcatgcag aggacgggga tcgatggcct
    75721 ccgctttgcc gttttgtaca cgaatcgtca tcagcagcgg cgcgaaatct ggcgtaacga
    75781 aaattttagt ttcgtttttc ccctctttcg gtgaatccgc gttcggaccg cgatcggtaa
    75841 tggttgcgaa tgtcagggca tcgccctgtt taccggtgaa cagcaggcca gaacctatcc
    75901 caaccggaag gccgttcggg aatgcactgg caaatgcgcc ggcgtagttc acgtgcgtgc
    75961 cttctggaaa actgacgaca tagcgctcag ccgtaggttg tgccgccagg gcagagaaag
    76021 agagcgtaca accgattagc acgggaataa tttttctttt catattgtta gcatccataa
    76081 agtgaagatc acactacacc ataaagaaga aacgagtcag gaaaatgaca tcgaatggca
    76141 acatgcgcgg gcctgaggcc aaagaaatgt aaaacactgt tgttatttgt aacaacggaa
    76201 acatttacgg aaattatttg tctttcttcg tgatcaaaaa tgctgtgtct tgttggattg
    76261 gagtgatgag ggctgcgttt aggaaacgcc ctatattgta cggttgcatt atcgcggtag
    76321 gggtaatgca cgtgtcagta tgatgagctg accacactca cgtgggtgga ctggtataac
    76381 aatcgacgat tgctggaaag gcagggccat atcccaccgg cagaagcaga aaaagcttat
    76441 tatgcttcca tcggaaacga tgatctggca gcctgagttc acagataaaa cactctccag
    76501 gaaacccggg gcggttcact tagccgcgtg tccattattg ctggataaga tcataacgat
    76561 acaccgggtt aaacaggtgg tcactgcttc actgaagctg cgtgattatg atggtcaggt
    76621 agcagaagct atgcccatga taccaaaatg acgaaagccg aaatgtctga aagcgtgtgt
    76681 attacctgaa aatatgaccg gctccggggt acgtaaccga ttcaacaaag ccaatcgata
    76741 ttacaaaacg agagggacta tatatttcgt tttcagataa gactccttga gagattatct
    76801 tttatgtaaa atctcaaata tcacctgatg taatgtcaat gataatatta agtgcgctct
    76861 gaacaacatc tttcttggtg tgattaacat ctgacaaaaa gaacatcagc ttctttatta
    76921 tgttcttgtt gtttattatt tcacgctcac agaataaagc gttgactgcc tcttctatga
    76981 catttctctc gtaaaaaaat tgagagttag cttcatataa aatattggcc aatttttcac
    77041 caaaaaccat attataataa tcactgtgca tctttctccc ccagggttca gtcaataatt
    77101 ttaccatgcc taattatgac aggtgtaaca agcatatttt gctatataaa taaaatacat
    77161 aatactgatt attgtaagaa caggaagcag actaatggtt ttttttgtga gcacattcct
    77221 tcttatgtag gaagaggctg tattatattc agtcaactct tttttactaa aaacattatc
    77281 tatgccatca aagttgcctg ctagctaatt tttaggatat cgccttgtc
//
LOCUS       DAMZET010000006        74270 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.006, whole genome shotgun sequence.
ACCESSION   DAMZET010000006 DAMZET010000000
VERSION     DAMZET010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 74270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.006"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(48..424)
                     /gene="rnpB"
                     /locus_tag="POB34_000474"
     ncRNA           complement(48..424)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="POB34_000474"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(457..1602)
                     /gene="garK"
                     /locus_tag="POB34_000475"
     CDS             complement(457..1602)
                     /gene="garK"
                     /locus_tag="POB34_000475"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HDI5917479.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1699..2589)
                     /gene="garR"
                     /locus_tag="POB34_000476"
     CDS             complement(1699..2589)
                     /gene="garR"
                     /locus_tag="POB34_000476"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDI5917480.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2619..3389)
                     /gene="garL"
                     /locus_tag="POB34_000477"
     CDS             complement(2619..3389)
                     /gene="garL"
                     /locus_tag="POB34_000477"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HDI5917481.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     IDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     IEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3405..4739)
                     /gene="garP"
                     /locus_tag="POB34_000478"
     CDS             complement(3405..4739)
                     /gene="garP"
                     /locus_tag="POB34_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HDI5917482.1"
                     /translation="MILDTVDVKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWSVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALIFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5114..6685
                     /gene="garD"
                     /locus_tag="POB34_000479"
     CDS             5114..6685
                     /gene="garD"
                     /locus_tag="POB34_000479"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HDI5917483.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGGWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNIGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            6834..7169
                     /gene="prlF"
                     /locus_tag="POB34_000480"
     CDS             6834..7169
                     /gene="prlF"
                     /locus_tag="POB34_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HDI5917484.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7169..7633
                     /gene="yhaV"
                     /locus_tag="POB34_000481"
     CDS             7169..7633
                     /gene="yhaV"
                     /locus_tag="POB34_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HDI5917485.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(7688..8497)
                     /gene="agaR"
                     /locus_tag="POB34_000482"
     CDS             complement(7688..8497)
                     /gene="agaR"
                     /locus_tag="POB34_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HDI5917486.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            8746..10026
                     /gene="kbaZ"
                     /locus_tag="POB34_000483"
     CDS             8746..10026
                     /gene="kbaZ"
                     /locus_tag="POB34_000483"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HDI5917487.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10049..10522
                     /gene="agaV"
                     /locus_tag="POB34_000484"
     CDS             10049..10522
                     /gene="agaV"
                     /locus_tag="POB34_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDI5917488.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            10533..11312
                     /locus_tag="POB34_000485"
     CDS             10533..11312
                     /locus_tag="POB34_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HDI5917489.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11302..12180
                     /locus_tag="POB34_000486"
     CDS             11302..12180
                     /locus_tag="POB34_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDI5917490.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12198..12632
                     /locus_tag="POB34_000487"
     CDS             12198..12632
                     /locus_tag="POB34_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDI5917491.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            12629..13762
                     /gene="nagA"
                     /locus_tag="POB34_000488"
     CDS             12629..13762
                     /gene="nagA"
                     /locus_tag="POB34_000488"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDI5917492.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLCIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEKVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            14113..15267
                     /locus_tag="POB34_000489"
     CDS             14113..15267
                     /locus_tag="POB34_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HDI5917493.1"
                     /translation="MPKNYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDDET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESSDIVAASPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15280..16140
                     /gene="kbaY"
                     /locus_tag="POB34_000490"
     CDS             15280..16140
                     /gene="kbaY"
                     /locus_tag="POB34_000490"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HDI5917494.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            16307..16783
                     /gene="agaB"
                     /locus_tag="POB34_000491"
     CDS             16307..16783
                     /gene="agaB"
                     /locus_tag="POB34_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDI5917495.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            16822..17625
                     /gene="agaC"
                     /locus_tag="POB34_000492"
     CDS             16822..17625
                     /gene="agaC"
                     /locus_tag="POB34_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HDI5917496.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            17615..18406
                     /gene="agaD"
                     /locus_tag="POB34_000493"
     CDS             17615..18406
                     /gene="agaD"
                     /locus_tag="POB34_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HDI5917497.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            18407..19162
                     /locus_tag="POB34_000494"
     CDS             18407..19162
                     /locus_tag="POB34_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HDI5917498.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
     gene            19563..20147
                     /locus_tag="POB34_000495"
     CDS             19563..20147
                     /locus_tag="POB34_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDI5917499.1"
                     /translation="MNKVTKTAIAGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSAGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            20227..20922
                     /locus_tag="POB34_000496"
     CDS             20227..20922
                     /locus_tag="POB34_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDI5917500.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDNVNANELTLVFKTRIKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            20952..23468
                     /locus_tag="POB34_000497"
     CDS             20952..23468
                     /locus_tag="POB34_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDI5917501.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYSGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSVNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            23479..24570
                     /locus_tag="POB34_000498"
     CDS             23479..24570
                     /locus_tag="POB34_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI5917502.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLKANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(24613..25473)
                     /gene="rsmI"
                     /locus_tag="POB34_000499"
     CDS             complement(24613..25473)
                     /gene="rsmI"
                     /locus_tag="POB34_000499"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDI5917503.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            25538..27574
                     /gene="lpoA"
                     /locus_tag="POB34_000500"
     CDS             25538..27574
                     /gene="lpoA"
                     /locus_tag="POB34_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HDI5917504.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            27532..27927
                     /locus_tag="POB34_000501"
     CDS             27532..27927
                     /locus_tag="POB34_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDI5917505.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            27947..28537
                     /gene="diaA"
                     /locus_tag="POB34_000502"
     CDS             27947..28537
                     /gene="diaA"
                     /locus_tag="POB34_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HDI5917506.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            28547..29122
                     /gene="yraP"
                     /locus_tag="POB34_000503"
     CDS             28547..29122
                     /gene="yraP"
                     /locus_tag="POB34_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HDI5917507.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(29327..30367)
                     /locus_tag="POB34_000504"
     CDS             complement(29327..30367)
                     /locus_tag="POB34_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDI5917508.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWEPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGTVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(30440..31075)
                     /locus_tag="POB34_000505"
     CDS             complement(30440..31075)
                     /locus_tag="POB34_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDI5917509.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAESMRPEHEGVTILSSSELRKRAE"
     gene            31203..31721
                     /gene="yhbO"
                     /locus_tag="POB34_000506"
     CDS             31203..31721
                     /gene="yhbO"
                     /locus_tag="POB34_000506"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HDI5917510.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(31701..32144)
                     /locus_tag="POB34_000507"
     CDS             complement(31701..32144)
                     /locus_tag="POB34_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HDI5917511.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            32195..32497
                     /gene="yhbQ"
                     /locus_tag="POB34_000508"
     CDS             32195..32497
                     /gene="yhbQ"
                     /locus_tag="POB34_000508"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HDI5917512.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(32484..32987)
                     /locus_tag="POB34_000509"
     CDS             complement(32484..32987)
                     /locus_tag="POB34_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI5917513.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(32981..33505)
                     /locus_tag="POB34_000510"
     CDS             complement(32981..33505)
                     /locus_tag="POB34_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HDI5917514.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            33714..34709
                     /locus_tag="POB34_000511"
     CDS             33714..34709
                     /locus_tag="POB34_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDI5917515.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            34718..35596
                     /locus_tag="POB34_000512"
     CDS             34718..35596
                     /locus_tag="POB34_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDI5917516.1"
                     /translation="MKYSLGPVLWYWPKETLEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            35802..36809
                     /locus_tag="POB34_000513"
     CDS             35802..36809
                     /locus_tag="POB34_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDI5917517.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPNVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(36927..38171)
                     /gene="mtr"
                     /locus_tag="POB34_000514"
     CDS             complement(36927..38171)
                     /gene="mtr"
                     /locus_tag="POB34_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HDI5917518.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(38325..40214)
                     /gene="deaD"
                     /locus_tag="POB34_000515"
     CDS             complement(38325..40214)
                     /gene="deaD"
                     /locus_tag="POB34_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HDI5917519.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(40207..40287)
                     /gene="yrbN"
                     /locus_tag="POB34_000516"
     CDS             complement(40207..40287)
                     /gene="yrbN"
                     /locus_tag="POB34_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HDI5917520.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(40394..41278)
                     /gene="nlpI"
                     /locus_tag="POB34_000517"
     CDS             complement(40394..41278)
                     /gene="nlpI"
                     /locus_tag="POB34_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HDI5917521.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(41387..43522)
                     /gene="pnp"
                     /locus_tag="POB34_000518"
     CDS             complement(41387..43522)
                     /gene="pnp"
                     /locus_tag="POB34_000518"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDI5917522.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(43769..44038)
                     /gene="rpsO"
                     /locus_tag="POB34_000519"
     CDS             complement(43769..44038)
                     /gene="rpsO"
                     /locus_tag="POB34_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDI5917523.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(44187..45131)
                     /gene="truB"
                     /locus_tag="POB34_000520"
     CDS             complement(44187..45131)
                     /gene="truB"
                     /locus_tag="POB34_000520"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDI5917524.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(45131..45532)
                     /gene="rbfA"
                     /locus_tag="POB34_000521"
     CDS             complement(45131..45532)
                     /gene="rbfA"
                     /locus_tag="POB34_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDI5917525.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(45696..48368)
                     /gene="infB"
                     /locus_tag="POB34_000522"
     CDS             complement(45696..48368)
                     /gene="infB"
                     /locus_tag="POB34_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDI5917526.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(48393..49880)
                     /gene="nusA"
                     /locus_tag="POB34_000523"
     CDS             complement(48393..49880)
                     /gene="nusA"
                     /locus_tag="POB34_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDI5917527.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(49908..50360)
                     /gene="rimP"
                     /locus_tag="POB34_000524"
     CDS             complement(49908..50360)
                     /gene="rimP"
                     /locus_tag="POB34_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDI5917528.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(50567..50643)
                     /locus_tag="POB34_000525"
     tRNA            complement(50567..50643)
                     /locus_tag="POB34_000525"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50607..50609),aa:Met,seq:cat)
     gene            50991..52334
                     /gene="argG"
                     /locus_tag="POB34_000526"
     CDS             50991..52334
                     /gene="argG"
                     /locus_tag="POB34_000526"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDI5917529.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(52342..53967)
                     /locus_tag="POB34_000527"
     CDS             complement(52342..53967)
                     /locus_tag="POB34_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDI5917530.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVILFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(54426..54512)
                     /locus_tag="POB34_000528"
     tRNA            complement(54426..54512)
                     /locus_tag="POB34_000528"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54476..54478),aa:Leu,seq:gag)
     gene            complement(54527..54859)
                     /gene="secG"
                     /locus_tag="POB34_000529"
     CDS             complement(54527..54859)
                     /gene="secG"
                     /locus_tag="POB34_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDI5917531.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(55087..56424)
                     /gene="glmM"
                     /locus_tag="POB34_000530"
     CDS             complement(55087..56424)
                     /gene="glmM"
                     /locus_tag="POB34_000530"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDI5917532.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(56417..57265)
                     /gene="folP"
                     /locus_tag="POB34_000531"
     CDS             complement(56417..57265)
                     /gene="folP"
                     /locus_tag="POB34_000531"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDI5917533.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(57355..59289)
                     /gene="ftsH"
                     /locus_tag="POB34_000532"
     CDS             complement(57355..59289)
                     /gene="ftsH"
                     /locus_tag="POB34_000532"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDI5917534.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(59389..60018)
                     /gene="rlmE"
                     /locus_tag="POB34_000533"
     CDS             complement(59389..60018)
                     /gene="rlmE"
                     /locus_tag="POB34_000533"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HDI5917535.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            60144..60437
                     /gene="yhbY"
                     /locus_tag="POB34_000534"
     CDS             60144..60437
                     /gene="yhbY"
                     /locus_tag="POB34_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDI5917536.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(60593..61069)
                     /gene="greA"
                     /locus_tag="POB34_000535"
     CDS             complement(60593..61069)
                     /gene="greA"
                     /locus_tag="POB34_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDI5917537.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            61317..62750
                     /gene="dacB"
                     /locus_tag="POB34_000536"
     CDS             61317..62750
                     /gene="dacB"
                     /locus_tag="POB34_000536"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDI5917538.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(62936..64108)
                     /gene="cgtA"
                     /locus_tag="POB34_000537"
     CDS             complement(62936..64108)
                     /gene="cgtA"
                     /locus_tag="POB34_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HDI5917539.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(64124..65089)
                     /locus_tag="POB34_000538"
     CDS             complement(64124..65089)
                     /locus_tag="POB34_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDI5917540.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(65216..65473)
                     /gene="rpmA"
                     /locus_tag="POB34_000539"
     CDS             complement(65216..65473)
                     /gene="rpmA"
                     /locus_tag="POB34_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDI5917541.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(65494..65805)
                     /gene="rplU"
                     /locus_tag="POB34_000540"
     CDS             complement(65494..65805)
                     /gene="rplU"
                     /locus_tag="POB34_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDI5917542.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            66064..67035
                     /gene="ispB"
                     /locus_tag="POB34_000541"
     CDS             66064..67035
                     /gene="ispB"
                     /locus_tag="POB34_000541"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HDI5917543.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            67262..67540
                     /gene="sfsB"
                     /locus_tag="POB34_000542"
     CDS             67262..67540
                     /gene="sfsB"
                     /locus_tag="POB34_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HDI5917544.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(67588..68847)
                     /gene="murA"
                     /locus_tag="POB34_000543"
     CDS             complement(67588..68847)
                     /gene="murA"
                     /locus_tag="POB34_000543"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDI5917545.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(68902..69156)
                     /gene="ibaG"
                     /locus_tag="POB34_000544"
     CDS             complement(68902..69156)
                     /gene="ibaG"
                     /locus_tag="POB34_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HDI5917546.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(69316..69609)
                     /gene="mlaB"
                     /locus_tag="POB34_000545"
     CDS             complement(69316..69609)
                     /gene="mlaB"
                     /locus_tag="POB34_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HDI5917547.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(69609..70244)
                     /gene="mlaC"
                     /locus_tag="POB34_000546"
     CDS             complement(69609..70244)
                     /gene="mlaC"
                     /locus_tag="POB34_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HDI5917548.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(70263..70814)
                     /gene="mlaD"
                     /locus_tag="POB34_000547"
     CDS             complement(70263..70814)
                     /gene="mlaD"
                     /locus_tag="POB34_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HDI5917549.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(70819..71601)
                     /gene="mlaE"
                     /locus_tag="POB34_000548"
     CDS             complement(70819..71601)
                     /gene="mlaE"
                     /locus_tag="POB34_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HDI5917550.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(71609..72418)
                     /gene="mlaF"
                     /locus_tag="POB34_000549"
     CDS             complement(71609..72418)
                     /gene="mlaF"
                     /locus_tag="POB34_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HDI5917551.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            72628..73605
                     /locus_tag="POB34_000550"
     CDS             72628..73605
                     /locus_tag="POB34_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDI5917552.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            73619..>74270
                     /locus_tag="POB34_000551"
     CDS             73619..>74270
                     /locus_tag="POB34_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HDI5917553.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIP"
BASE COUNT        18242 a        19872 c        18325 g        17831 t
ORIGIN      
        1 gctcctccaa aagcaaaaac ccgccgaagc gggtttttac gtaaatcagg tgaaactgac
       61 cgataagccg ggttctgtcg tggacagtca ttcatctagg ccagcaatcg ctcactggct
      121 caagcagcct acccgggttc agtacgggcc gtaccttatg aacccctatt tggccttgct
      181 ccgggtggag tttaccgtgc cacggactgt taccagccgc gcggtgcgct cttaccgcac
      241 cctttcaccc ttacctgatc ccgcttgcgc gggccatcgg cggtttgctc tctgttgcac
      301 tggtcgtggg tttccccccc aggcgttacc tggcaccctg ccctatggag cccggacttt
      361 cctcccctcc gcccgtctcc cccgaagagg acgacgacga agcggcgact gtctggtcag
      421 cttcggcgcg cagtatagag ggtttgcgcg cccttgtcac cccgcgttgc gcattccaat
      481 cgccagtgtc gcggcgatat tacgtgaagc acggcagata ttgtcataag ccccgcggaa
      541 tgcttcgtcc aacgtaccta tgctggtcaa tacgctgaag accgcatcga tgccatgctg
      601 atgtacaacg ccaacatcat cggtcaggct acccgcaatg ccaatcaccg gtttatggta
      661 cttcttcgcc acgtttgcaa cgccaatcgg taccttgccg tgaatactct ggctgtcaat
      721 acgcccttca ccggtgatca ccagcgtaca atcgtgaata tgttcctcca gattcagcgc
      781 cgtagtgacg atttcaatac cacttttcag ttccgcacca agaaacgcca tcagcgccgc
      841 acccataccg cccgcagctc ctgcgccggg gacatctttc acatcaacat gcagcgcttt
      901 tttaatgaca tcggcatagt gagagaggtt attgtccagc tcaacaatca tcgcttcact
      961 ggctcccttt tgtgggccaa agatgcgcga tgcgccgtta tcgcccacca gcggattggt
     1021 gacatcacaa gcgacgcgaa tgacgcaatc ttttaagcgc ggatcgaggc cggaaatatc
     1081 aatatcattc agagtattaa gactaccgcc gccaaaacca atttcattgc cgttggcgtc
     1141 gcataatttc gcccccagcg cctgtaccat gcctgcgccg ccatcatttg tagcgctacc
     1201 gccaatgccg ataataatgt ttgtcgcgcc gctctccagc gcctgcagga ttaactcgcc
     1261 tgtgccgcgt gaagtggtca cgagtggatc gcgtttttcc gcaggtacca gctccagccc
     1321 actggccgcc gccatttcaa taaacgcggt tttgccatcg ccggagatcc cccaactggc
     1381 attcactttc tcgcccagcg gccctgtaac ccaggcgtga cgttcagaac cctgggtggc
     1441 tgcaatcatc gcttccaccg ttccttcgcc accgtcggca accggaatag aaacgtactg
     1501 tgcatcagga aaaatttccc gaaatccttt ttctatcgcc tgcgcaacct cgctggcaga
     1561 taaactttct ttataagagt ctggggcgat tacgattttc atacctatgc ctgttaccac
     1621 atgacgccgg agggcgtttc tcttattcgg cctggattcc aggcccggat tgcaatacgc
     1681 catccgggca cgacgtcatt aacgagtaac ttcgactttc gccagttttt cgtagtagca
     1741 cgccagggcg ctatgatccg ccgttcctaa accatctgct cgcagtgcct gcatcatctc
     1801 cataaccgca gctgtgagcg gcagttgcgc gccgacgccg tgggaagtat ccagcgcatt
     1861 tgccagatcc ttaatatgca gatcaatacg gaagcccggc ttgaagttgc ggtccatcac
     1921 catcggcgct ttggcatcca gcacggtact gcccgccagt ccaccgcgaa ttgcctgata
     1981 caccaggtcc gggttaacgc ccgctttggt tgccagcgtt agcgcttctg acatcgcggc
     2041 aatattcagc gccacaatga cctgatttgc cagtttggtg acgttacctg caccgatttc
     2101 cccggtatgc accacggaac ccgccatcgc tttcatcaaa tcatagtatt tgtcgaaaat
     2161 agccttgtcg ccgcccacca tcactgacag cgtaccgtcg atggctttcg gttcaccgcc
     2221 gctcaccgga gcatccagca tatcaatgcc tttcgctttc agcgcttcgc tgatttcacg
     2281 gcttgccagc ggtgcgatag aactcatatc gatcaatacc gtacctggct tcgcgccttc
     2341 aatgatgcca ttctcaccca gcgccacctc tttcacatga ggggagtttg gcagcatggt
     2401 tatgatgacg tcgcactgtt cagcgatcgc tttagccgta gacgctgttt ctgcacctgc
     2461 agcaatcacg tcagcaatag cttctgggtt acggtcagca accaccagcg agtaacctgc
     2521 tttcagaagg tttttactca ttggtttacc cataatcccc aggccaataa aaccaacttt
     2581 catagtcata tcaatcatct ctcttgttgc ggtggtggtt attttttaaa ggtatcagcc
     2641 agtttctgag tggcagagcg gaagacgccg agatcgctgc cgacagccac aaacgtcgcg
     2701 ccccattcca gataacgacg cgcatcggct tcgatcggtg cgaggatacc gctgggtttg
     2761 ccgtgcgcac tggcacggtt aaaaatgtgc tgaattgctt tttgtacatc cgggtgcgag
     2821 gcattgccga gatggcctaa tgccgcagcc agatcgctgg ggccgacgaa gatgccgtct
     2881 acgccttcgg tagcggcaat ggcatcgatg ttatctacgc cctgctgact ttctatctgg
     2941 accagaatag tgatgttctt gttcgactga gcgaaataat ccgccacggt gccaaacata
     3001 ttggcgcggt gagaaacgga gacgccgcga atgccttccg gtgggtaacg ggttgatgcc
     3061 accgcctgct ctgcttcctc ttttgtttct acaaaaggaa tcaggaagtt atagaaaccg
     3121 atatccagaa gacgcttaat aattaccggc tcgttggtcg gcactcgcac tactggcgcg
     3181 ctggcgctgc ctttcagggc cattaactgc ggaataaacg tggaaatatc gtttggcgca
     3241 tgttcgccat ccagcaccag ccagtcaaac ccagccaaac caagaacttc agtgctaatc
     3301 gggttagaga gtgctgacca gcaaccaatt tgtacctgtt tcgcagccag tgcggctttg
     3361 aatttattcg ggaaaacatc gttattcatc gcttatacct ttgcttattt ctgcaattcc
     3421 atacgtttaa tgtcgccaac tacgaagagg tagcagacca tcgccatcag cgctgaacat
     3481 cccacgaaaa tcagtgctgc attgaaggag tgcagttcac ttaccaggta gccaatcacc
     3541 agtggagtga caatggaggc aacattgcca aagacgttaa agacgccgcc gcagaggcca
     3601 acaatctctt tcggcgcggt gtcagaaatc accgaccagc ccagagcacc aaatcctttg
     3661 ccaaaaaaag ccagcgccat cagcatgacc accagcgtgg tgttgttggt gtagttacat
     3721 aagatgatgg tggaagccag caacattccc agcacaatcg gtagcttacg tgccagggtc
     3781 agggataaac cgcgtttgat cagataatcc gagaagacac ctcccagcac gccgcccgca
     3841 aaaccacaca gtgccggaat cgaggcgacc agacccactt tcagaatcga catgcctttt
     3901 tcctgcacca gataaatcgg gaaccaggtg aggaagaacc aggtgatggt gttgataaaa
     3961 tattgtccga aaaatacgcc caacatcatg cggttagaga gcaattgctt gatgtaatgc
     4021 agtttgggac cgcttgctgc cgcgctgccc ggctttttgt ggtccatatc gaccaccgcg
     4081 ccattttcag agataaactt cagctcttcc gcagacatac gtgggtgatc tgtcgggtta
     4141 tgaatcaact tgatccacag cgccgtcagc acaaaaccaa tcacccccat aacggtgaag
     4201 acgtgctccc agccccaggc gaaagtcagc cagccaagca gcggcgaaaa gagcgccagc
     4261 gagaaatatt gcgccgagtt aaagatggcg gaggcagtac cacgttcttt cgtcgggaac
     4321 caggcggcga caatacgggc gttcgccggg aatgatggcg cttccgagaa ccccagcata
     4381 aagcgcataa agaacatgga gatccctgcc caggccagcg ggaacatatc aacaaagcct
     4441 tgcaggaagg tgaacagcga ccagaaaaag aggctgtagg tgtaaacttt tttcgagcca
     4501 aacttatcaa gcagccagcc gccgggaatt tgcatcagca ggtaggccca gccaaaagcg
     4561 gagaagatgt aacccatcga aaccgcactt aactgcaact cttttgccac ttcggtacca
     4621 gcaatagaca gcgttgcacg atcggcgtag ttaacggcgg taacaataaa aataatcagt
     4681 aatattaaat agcgggtatg cacgcctttc ttttttacgt caacggtgtc cagaatcatt
     4741 ttatttacct cgggtactta tgctgatttt tattattatg gtgaaggtgt tatttatgag
     4801 ttttatttat gccgttacga caatgaactc gggaattagt ataagcagcg cgagaataat
     4861 aatcattgtg caaatgctaa tttaattaat accatttaaa tattattttg agcatatgca
     4921 cataaggttg cgcgctaaag cacagatttg cgctttacct tgccgggcgg cactgcaatc
     4981 cctgaaatga ttgacattga tcacatttct gcgtttaaac tcctgacatt cttatttcac
     5041 ccaatgaagt catttatttt taaatgagac caggtcctca ttttaataac ccctggctgg
     5101 agaatattgc acaatggcca acatcgaaat cagacaagaa acgccaactg cgttttatat
     5161 aaaagttcac gacacagata atgtggcaat tattgttaat gataatggcc tgaaagcagg
     5221 aacgcgtttt ccggatgggc tggaattaat tgaacatatt ccccaggggc ataaagtcgc
     5281 attgctggac attccggcta atggtgaaat tattcgttat ggcgaagtga ttggttacgc
     5341 cgtgcgcgca atcccacgcg gaggctggat cgacgaatca atggttgtac taccggaagc
     5401 gccgccgtta cacacgctgc cactggcaac caaagtcccg gaacccttac cgccgctgga
     5461 aggatacacc tttgagggct atcgcaatgc cgatggcagc gtgggcacca aaaacctgct
     5521 cggcatcacc accagcgtcc actgtgtggc aggcgtggtg gactacgtag taaaaatcat
     5581 tgaacgcgat ctgctaccga aatacccgaa cgtcgatggc gtggtggggc tgaatcattt
     5641 gtacggttgt ggcgtggcga ttaacgcacc ggcggcagtt gtgcctattc gtaccattca
     5701 caatatttcg ctgaacccta actttggcgg cgaagtaatg gtgattggcc tgggttgtga
     5761 aaagttgcag cctgagcgcc tgctgactgg aacggatgat gtgcaagcta ttccagtaga
     5821 aagcgccagc attgtcagtt tgcaggatga aaagcatgtc ggttttcagt ccatggtcga
     5881 ggatattttg caggtcgccg aacgccatct acaaaaactg aatcaacgtc agcgagaaac
     5941 ctgtccggct tcagaactgg ttgtcggcat gcagtgcggt ggcagcgatg ccttttctgg
     6001 cgtaacggca aacccggcgg ttgggtatgc gtctgatcta ctggtgcgct gcggcgcaac
     6061 ggtgatgttc tcagaagtca cggaagtgcg tgacgcgatc catctgctga caccacgcgc
     6121 agtgaacgaa gaggtcggca aacggctgct ggaggagatg gagtggtacg ataactatct
     6181 caatatagga aaaaccgacc gcagcgccaa cccttcgccg ggcaacaaga aaggcggtct
     6241 ggcaaacgtg gtagagaagg cactcggctc cattgctaaa tcgggtaaaa gcgcaattgt
     6301 tgaagtgctg tcgcccggtc aacgcccgac taaacgcgga ttaatttacg ccgcgacgcc
     6361 agccagcgat tttgtctgtg gcacgcaaca ggtggcttcg ggtatcaccg tgcaagtgtt
     6421 tacgaccggt cgtggtacgc cgtacggcct gatggcggta cccgtcatta aaatggcgac
     6481 ccgcaccgag ctggcgaacc gctggtttga tttaatggat attaacgcgg gcactatcgc
     6541 caccggcgaa gaaaccattg aagaggtggg ctggaagttg ttccacttta ttctcgacgt
     6601 cgccagcggg aagaagaaaa ccttctcgga tcaatggggg ctgcataacc agctggcagt
     6661 gtttaacccg gcaccggtga cctgatttct tttggaacgt ccctcgcaaa acatggcctt
     6721 agtgccatgt ttttattgtt taaagccccc acgtccatta ataatgcatt tgcattacct
     6781 tagttcaagc ttataatttg agcagaaaac aggctgtaaa aggacagtga atcatgcccg
     6841 ctaatgctcg ctctcacgct gtactgacca ctgaatcaaa ggtcacgata cgcggacaaa
     6901 caactatccc cgcgccagtg cgtgaggcct taaaactgaa gccaggccag gacagcattc
     6961 attacgaaat tctgcctggt gggcaagtct ttatgtgccg actgggagat gaacaggagg
     7021 atcatactat gaatgcattt ttgcgttttc tggatgcaga tatccagaac aacccgcaaa
     7081 aaactcgtcc attcaacatt caacaaggaa agaaacttgt cgctggcatg gacgtcaaca
     7141 ttgatgatga gattggcgac gacgaataat ggattttcca caaagggtta atggttgggc
     7201 gctatatgct catccctgtt ttcaggaaac ctacgatgct ttagttgccg aagtcgaggc
     7261 attaaaggga aaagatcctg aaaattatca gagaaaagct gccacaaagt tattggcggt
     7321 ggtccataaa gtgattgagg agcatatcac ggtcaatcca tcatcaccgg cattccgtca
     7381 tggcaagtcg ttaggctctg ggaaaaataa agactggtca cgggtaaaat ttggtgctgg
     7441 tcgctatcgt ctcttctttc gttacagtga aaaagagaaa gtcatcattc tgggatggat
     7501 gaacgatgaa aacacgctgc gcacctacgg taaaaaaaca gatgcctata ccgtattcag
     7561 taaaatgtta aaaagaggac atcctcctgc cgactgggaa agcctcaccc aagagacaga
     7621 agaaaaccat tgatggtgtt cacaatgccc gccgaacgta ttcgtgttga ccagcgggca
     7681 ccctttctca ctccccgacc agaatcactt caaccccagc ctttcgcagt ccttccagac
     7741 tatccgcagg aatgccttca tcaacaatga tcatgtcgat acgttgagta tcaatgatct
     7801 tatgtaaact ggaacgattg aacttactgg aatcggtgac tacgatgatc cgttccgcaa
     7861 cttcgcacat ccggcggttt aaacgggctt catcttcatt atgcgtgctg acgccgcgct
     7921 ccagatcgat cgcatctaca ccaagaaaca gcatatcgaa gtggtaattt tgcagcgatt
     7981 gttcagcctg atcgccgtaa aaagattgcg actgacggcg caaatgcccg ccggtcatca
     8041 gcagctcaac gccttccgct tccagcaaag cattagccac gttcataccg ttggtcatcg
     8101 caattacgtc agtgtgcttg cgcatcagac gagcaatctc aaaagtggta gtcccggaat
     8161 cgaggatcac ccgatgacct ggctgaatca actcaacggc agctttcgca acgctgcgtt
     8221 tcatcgcggt gttcagtgcg cttttatctt ccactgatgg ctcgactgac ggcgtcgtgc
     8281 tatcgcagat caacgcgcca ccataggcac gcacagcgat cccctgcttt tccagaaacg
     8341 ccagatcgtt gcggatcgtc acggtagata cgccatacaa tgccgacaga tcgttaacct
     8401 gcacactccc ttgctgtcgc agacgctgaa tgatctgttc tcgtcgctcg ctggtgcctg
     8461 tcactcgctt ctcacctgaa gcgtcggtat tactcatagt aagtcctttc gtaaaacttt
     8521 cgtttcattt cgttttgcct attaacgcct ttctattaag caaatgcaag cccaccttgc
     8581 ccattgacgc aagctactct cgtttcagtg actttcatta tgtttctttt gtgaatcaga
     8641 tcagaaaacc attatctttc gttttatttt tatctcacca tgacgcagta tcaactgaaa
     8701 caaaacgaaa gattaatatc gcagtaatct gaactggaga ggaaagtgaa acatctaaca
     8761 gaaatggtga gacagcacaa agcgggcaaa acaaatgcaa tttatgccgt ttgttccgca
     8821 catccgctgg tgctggaagc tgcaatccgc tacgccagtg caaaccaaac gccgttactg
     8881 attgaagcaa cctccaatca ggtagaccag ttcggcggtt ataccggaat gacgcccgcc
     8941 gattttcgcg gctttgtttg tcagctcgcc gactcgttga atttcccgca ggatgcgttg
     9001 attctgggtg gtgaccatct ggggccaaac cgctggcaaa acctgccagc cgctcaggca
     9061 atggccaatg ccgatgattt gattaaaagc tacgttgcgg caggattcaa aaaaatccac
     9121 cttgattgca gcatgtcctg tcaggacgat ccaattccct taactgatga catcgtggct
     9181 gaacgcgccg cccgtctggc gaaagtggcg gaagaaacct gtcttgaaca ctttggcgaa
     9241 gccgatctgg agtatgtcat tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc
     9301 ttaagcgagc tggcggtcac cacgccggat gccgcccgcg ccacgctgga agcccatcgt
     9361 cacgcctttg aaaagcaagg tttgaatgcc atctggccac gcatcattgc cctggtggtt
     9421 caacccggcg tcgaattcga tcacaccaac gttattgatt atcagcccgc caaagcgagc
     9481 gccttaagcc agatggtcga aaactacgaa acgctgattt tcgaagcgca ctctaccgat
     9541 tatcaaacgc cgcaatcgct gcgccagctg gtgattgacc actttgccat tctgaaagtt
     9601 ggcccagcgc tgaccttcgc cctgcgtgaa gctctgttct ctctggcggc gattgaagaa
     9661 gaactggtgc cagcgaaagc ctgttctggt ctgcgtcagg tgctggaaga cgtgatgctc
     9721 gaccgcccgg aatactggca aagccactac cacggtgacg gcaacgcgcg tcgtctggcg
     9781 cgtggttata gctactcgga tcgcgtgcgc tattactggc cggacagcca gattgatgac
     9841 gctttcgctc atctggtacg taatctggcg gattcaccaa ttccgctgcc gctgatcagc
     9901 cagtatctgc cgctgcagta cgtgaaagtt cgctccggcg agctgcagcc aacgccacgg
     9961 gaactcatta tcaaccatat tcaggacatc ctggcgcagt accacacagc ctgtgaaggc
    10021 caataagcaa aacaaagagg aacacgctat gccaaatatt gttttaagcc ggattgatga
    10081 acgcttgatt cacggtcagg tcggcgttca atgggtcgga tttgcggggg caaatctggt
    10141 gctggtagcc aacgatgagg ttgccgaaga tccggtacaa caaaacctga tggaaatggt
    10201 actggcagaa gggatcgccg tacgtttctg gacgctgcaa aaagttatcg acaacattca
    10261 tcgcgccgcc gatcgacaga aaatcctgct ggtttgtaaa acacccgccg atttcctgac
    10321 gctggtgaaa ggtggcgttc cggtgaatcg cattaacgtt ggcaatatgc actacgccaa
    10381 tggcaaacaa caaatcgcca aaacggtttc tgtggatgcg ggcgatatcg cagcatttaa
    10441 cgacctgaaa gccgctgggg tggaatgctt cgttcagggc gtcccgacag agcctgctgt
    10501 ggacctcttt aaattacttt gagggattca tcatggaaat cagcctgttg caggcatttg
    10561 cgttgggcat tatcgccttt atcgctggcc tggatatgtt taacggccta acacatatgc
    10621 accgcccggt ggtcctcggc ccgttggtcg ggctggtact tggcgatctg cataccggaa
    10681 ttttaaccgg cggtacgctg gaactggtgt ggatggggct ggccccgctg gcgggcgcac
    10741 agccgcctaa cgtgattatc ggtactatcg tcggcacggc gtttgccatt actactggcg
    10801 tgaaacccga tgtcgcagtc ggtgtcgccg tacctttcgc tgtcgcagta cagatgggga
    10861 ttaccttcct gttctcggtg atgtccggcg tgatgtctcg ctgcgaccgg atggcggaga
    10921 acgccgatac tcgcggcatt gaacgcgtga actatctggc actgctggca ctcggcactt
    10981 tctattttct ctgcgctttc ctgcctatct acttcggcgc ggaacatgca aaaaccatca
    11041 ttgatgtcct gccgcaacga ttaatcgacg gcctcggcgt cgcgggcggc atcatgccag
    11101 caatcggttt tgccgtgctg ctgaaaatca tgatgaaaaa cgtctacatc ccctacttca
    11161 tcctgggctt tgttgccgca gcctggctca agttaccggt gctggctatt gctgccgctg
    11221 cgctggcgat ggcgctgatc gacctgctgc gtaaatctcc tgaaccgact caacctgcgg
    11281 cacagaaaga ggaatttgaa gatggcatct aatcaaacca ccctgccgaa cgtcagtgaa
    11341 aacgaagaaa cattgctgac tggcgtcaat gaaaacgtgt atgaagatca gagcattggc
    11401 gcggagctga cgaaaaaaga tatcaaccgc gtcgcctggc gttccatgct gttacaagcg
    11461 tcttttaact acgaacgtat gcaggcttct ggctggttgt acggtctgct gcctgctctg
    11521 aaaaagatcc acactaataa acgcgacctg gcgcgcgcca tgaaggggca tatgggtttc
    11581 ttcaataccc atccgtttct ggtgacattt gttatcggca ttatccttgc gatggagcgt
    11641 tctaagcagg acgttaacag tattcagagc accaaaattg ccgtcggtgc gccgctcggc
    11701 gggattggcg atgcaatgtt ctggctaacg ctactgccga tttgtggcgg gattggagcc
    11761 agtctggcat tgcaaggctc cattcttggc gcagtcgtct ttattgtgct gttcaacgtg
    11821 gtacacctgg ggctgcgttt tggtctggcg cattatgctt accgcatggg cgtggcggcg
    11881 attccactca ttaaagcgaa taccaaaaaa gtcggccatg ctgcgtcaat cgttgggatg
    11941 acggtaatcg gcgcgctggt ggcaacctat gttcgtttga gcaccacgtt ggaaatcaca
    12001 gcgggcgacg cagtggttaa gttacaggct gatgttatcg acaaactgat gcctgccttc
    12061 ttaccgttgg tctacaccct gaccatgttt tggttggtac gccgcggctg gagtccgctg
    12121 cgtctgattg cagttaccgt ggttctcggc atcgtcggta aattctgcca tttcctttaa
    12181 atacaatgag gtattagatg ttaagtatta ttttgacagg gcatggcgga tttgccagcg
    12241 gcatggaaaa agcaatgaag caaatcctcg gcgagcaatc gcagtttatc gccatcgatt
    12301 ttccggaaac ctcaagcacc gcgctgctta catcgcagct tgaagaggcc atcgcccaac
    12361 tggattgtga agacggcatt gtttttctga cggatttgct gggcggcacg ccgtttcgcg
    12421 tagcttcaac gctggcgatg caaaaaccgg gctgtgaagt gatcaccggc accaatttgc
    12481 aactgctact ggagatggtg ctggagcgcg aagggttaag cggtgaagag ttccgtgtgc
    12541 aggcgctgga gtgtggacac cgtgggctga ccagtctggt agacgagtta ggccgctgcc
    12601 atgaagaatg tccggtcgag gaaggaatat gacacacgtt ctgcgcgcca gaaggctgct
    12661 gactgaagag ggatggctcg atgaccatca gttgtgtatt gctgacggtg taatcgcagc
    12721 aatcgaaccg attccagtgg gcgtgactga acgcgatgcg gaactgctct gccccgctta
    12781 catcgacacc catgtacacg gtggtgcggg cgttgatgta atggatgacg cgccggatgt
    12841 actcgacaag ctggcaatgc acaaggcacg cgaaggtgtc ggcagttggt tgccgactac
    12901 cgtaaccgcg ccgcttaaca ccattcatgc ggcgctgaaa cgtattgctc aacgttgcca
    12961 acgcggcgga cctggtgcgc aagtgctggg gagttatctc gaaggaccgt acttcacgcc
    13021 gcagaataaa ggcgcgcatc cgccggagct gttccgcgag ctggaaattg ccgagctgga
    13081 tcagttgatt gccgtttctc agcacacctt acgtgtggta gcgctggcac cggaaaaaga
    13141 aggggcattg caggccatcc gccatcttaa acagcaaaac gtaagggtga tgctggggca
    13201 tagcgcggcg acctggcaac aaacccgcgc cgcatttgat gctggtgccg acggcctggt
    13261 gcattgctat aacgggatga caggtttaca tcaccgcgaa ccgggaatgg ttggcgcggg
    13321 attaacggac aagcgcgcct ggctggaact gatagccgat ggtcatcatg tgcatccggc
    13381 ggcgatgtcg ctgtgttgtt gctgtgcgaa agagagaatc gtgctgatca ccgacgcgat
    13441 gcaggcagcc gggatgccgg atggtcgcta tacgttatgt ggtgaaaaag tgcagatgca
    13501 cggtggcgtt gtccgtaccg cgtctggtgg gctggcgggc agtacgctgt ctgttgatgc
    13561 ggcagtgcgc aacatggtcg agttgacggg cgtaacgcct gcggaagcca ttcatatggc
    13621 atcgctgcat ccggcgcgaa tgctgggtgt tgatggtgtt ctgggatcgc ttaaaccggg
    13681 caaacgcgcc agcatcgttg cgctggatag cgggctgcat gtgcaacaaa tctggattca
    13741 gagtcaatta gcttcgtttt gatagtttgc tcctttattg ggccttcact tcccccgtaa
    13801 ggcctttctt tttctttcgt tttgatctgt gcagcggtgt cggatgcgac gctaacgcgt
    13861 cttatgcgac ctacggtacg ccgcatccag caatcggtga acatagcgac gtcattacgc
    13921 aattctttaa tcttcccttg tcttttcttt caatgatcac agatttcgtt ttatttcctt
    13981 tttctccatt gaactttcgg tttcttttct atagatttta atcaacgaaa gacatcacca
    14041 agtgaaatga aacgaaaggc aagtgaaagc gacaacgccc gacgtcaagt tcatcagact
    14101 aaggattgag ttatgccaaa aaattacacc cctgctgccg ccgcaaccgg tacatggact
    14161 gaagaagaga tccgccatca gcctcgcgca tggatccgtt cactcaccaa catcgacgcg
    14221 ctacgttccg cgctcaataa cttccttgaa ccgttactgc gcaaagagaa tctgcggatc
    14281 atcctgaccg gagccggaac ctcggcattt atcggtgaca tcatcgcgcc gtggctcgcc
    14341 agccataccg gtaaaaactt cagcgccgta ccgaccaccg atctggtcac taatccgatg
    14401 gactacctga acccagccca tccgctgctg ttgatctcct tcggtcgatc cggcaacagc
    14461 ccggaaagcg tcgcagccgt ggaactggca aatcaatttg taccggaatg ctatcacctg
    14521 ccgatcacct gcaacgaagc gggcgctctt taccaaaacg cgatcaacag cgataacgcg
    14581 tttgccctgc tgatgcccgc agaaacgcac gatcgcggct ttgcgatgac cagcagcatt
    14641 accaccatga tggccagctg cctcgcggtt ttcgcacctg agacgatcaa cagccaaacc
    14701 ttccgcgacg tggcggatcg ttgccaggcg atcctgacct cactgggcga tttcagcgaa
    14761 ggtgtgtttg gttacgcacc gtggaaacgg atcgtttatc tcggtagcgg tggcttacag
    14821 ggcgcagcac gcgagtcggc gctgaaagtg ctggaactga cggcgggtaa actggcggcc
    14881 ttttatgatt ctccaaccgg attccgtcat ggaccaaaat cgctggtcga tgacgaaacg
    14941 ctggtggtgg tatttgtctc cagccaccct tacacccgtc agtatgatct tgatctgctg
    15001 gctgaacttc gccgtgacaa ccaggcaatg cgtgtaatcg ccatcgccgc ggaaagcagc
    15061 gacatcgtcg ctgccagtcc acatatcatc ctgccaccgt cacgtcactt tatcgacgtt
    15121 gagcaggcat tttgcttcct gatgtacgcc cagacgtttg cactgatgca gtcgctgcac
    15181 atgggcaata cgccggatac cccatcagcc agtggcaccg ttaaccgcgt ggtgcaaggc
    15241 gtaatcattc atccgtggca ggcataagag gatcgcatta tgagcattat ctccactaaa
    15301 tatctgttac aggacgccca ggccaatggc tacgcggtgc ctgcttttaa cattcataac
    15361 gccgagacga tccaagcgat cctcgaagtg tgcagtgaaa tgcgatcgcc ggtgatcctc
    15421 gccggaacgc cggggacctt taaacacatc gcgctggaag agatctacgc cctgtgtagc
    15481 gcctattcca caacctacaa catgccactg gcgctgcatc tcgaccacca cgaatcgctg
    15541 gatgatattc gccgtaaagt ccacgcaggc gtgcgcagtg cgatgatcga cggcagccac
    15601 ttcccgtttg ccgagaacgt aaagctggtg aaatcggttg tcgacttctg ccactcccag
    15661 gattgcagcg tggaagcaga actgggccgc ctgggcggtg ttgaagatga catgagcgtt
    15721 gacgccgaaa gtgcattcct gaccgatcca caagaagcta aacgctttgt cgaactgact
    15781 ggcgtcgaca gcctggcggt agcgattggt acggcgcacg gcttatacag caaaacgccg
    15841 aagattgatt tccagcggct ggcggaaatt cgtgaagtgg tggatgttcc tctggtgctg
    15901 catggtgcca gcgatgttcc ggatgaattt gtccgtcgca ctattgaact tggcgtcaca
    15961 aaagtgaacg ttgccacaga attaaaaata gccttcgctg gcgcggttaa agcctggttt
    16021 gcggaaaatc cgcagggtaa tgatcctcgt tattatatgc gcgtcggaat ggatgcgatg
    16081 aaagaagttg tcagaaataa aattaatgtc tgtggttcag cgaatcgaat ttcagcataa
    16141 tcattagttt tttatgattt atcccaatgt acttcccgat taatatcggg gagtgcctta
    16201 atggaaaagg agataactaa accttaataa atacatcagt acaatatcgc aacaataata
    16261 tatttaaaaa aattatatta ttcaacttta tggtgaggat tacacaatga gcagtccaaa
    16321 tattctctta acccgtattg ataaccgtct ggttcatggc caggttggcg tgacctggac
    16381 atccaccatc ggtgcaaatc tgctggtagt cgtggatgat gttgtcgcta acgatgatat
    16441 tcaacagaaa ttaatgggta ttaccgcgga aacctacggc tttggcattc gtttctttac
    16501 tatcgaaaaa accattaacg tcatcggcaa agctgcacca catcagaaga tcttcctgat
    16561 ttgccgtacg ccacaaacgg tacgtaaatt ggtagaaggt ggcattgacc tgaaagatgt
    16621 caacgtcggc aatatgcatt tctcggaagg gaaaaagcaa atcagcagta aagtttatgt
    16681 cgatgaccag gatctcacgg acttacgttt tattaaacaa cgtggcgtga atgttttcat
    16741 tcaggatgtc cctggcgatc aaaaagaaca aatccctgac taaatctaaa atcgccttaa
    16801 tattggtttg aggtaataaa aatgcatgaa ataaccctac ttcagggatt atccctggcg
    16861 gcgttagttt tcgttctggg gattgatttt tggctggaag ccttattttt attccgcccg
    16921 ataatcgttt gtacccttac aggtgctatt ctcggtgata ttcagactgg cttaattacc
    16981 ggtggtctga cagagttggc tttcgccgga ttaacccctg caggtggtgt tcagccgccc
    17041 aacccgatta tggcgggtct gatgaccacc gtcattgcat ggtctacggg cgttgatgcc
    17101 aaaacagcaa ttggtcttgg cctgccgttt agtttgttaa tgcagtacgt cattctgttc
    17161 ttctattccg ctttctcatt atttatgacc aaagccgata aatgcgcgaa agaggcggat
    17221 acggcagcgt tttcccggct taactggaca acgatgctca tcgtcgcttc agcgtatgcg
    17281 gtgattgctt tcctctgtac ttacctggca caaggggcga tgcaggcgct ggtgaaagcg
    17341 atgcccgcct ggctgaccca cggctttgaa gtggctggcg gtattctgcc tgccgttggt
    17401 tttggcttgc tgctgcgcgt gatgttcaaa gcacaatata tcccttacct gatcgccggc
    17461 ttcctgtttg tttgctacat ccaggtcagt aacctgttgc cggttgccgt actgggcgca
    17521 ggctttgcgg tgtatgagtt tttcaatgcg aaatcccggc agcaagcgca accgcagccc
    17581 gttgccagta aaaatgaaga agaggactac agcaatggga tctgaaatca gtaaaaaaga
    17641 tatcacccgt ctgggctttc gttcgtcact gctgcaagcg agctttaact acgaaaggat
    17701 gcaggcgggc ggttttacct gggcgatgtt gccgatcctg aaaaagattt ataaggatga
    17761 caaaccgggc ctaagcgcag cgatgaaaga taacctcgaa tttattaata cccacccgaa
    17821 tctggtcgga ttcctgatgg ggttattaat ttcgatggaa gaaaaaggag aaaaccgcga
    17881 caccattaaa ggcctcaaag tggcactgtt tggcccaatc gccgggattg gcgatgcgat
    17941 tttctggttt actttgttgc cgattatggc gggaatttgc tcatcatttg ccagccaggg
    18001 aaacctgctg gggccgattc tatttttcgc cgtttacctg cttatctttt tcctgcgcgt
    18061 cggctggacc cacgtcggtt attcagtcgg cgtgaaggcg atcgataaag tacgagagaa
    18121 ctcgcagatg attgcccgtt cggcaaccat cctcgggatc acggtaatcg gcgggctgat
    18181 cgcttcgtat gtgcatatta acgtggtgac atcgtttgcc atcgacagta cccacagcgt
    18241 cgcgctgcag caggatttct tcgataaagt cttcccgaac attttaccga tggcctacac
    18301 cctgctgatg tattacttcc tgcgggtgaa aaaagcgcat ccggtgctgt taatcggcgt
    18361 gacttttgtg ctctctattg tttgttccgc attcggcatt ttgtaaatgg aacgaggcac
    18421 tgcgtctggt ggtgcctctt tacttaagga atttcatcct gtgcaaaccc ttcagcaagt
    18481 tgaaaactat acggcgttaa gtgaacgtgc cagcgaatat ttattggctg tgatccgtag
    18541 caaaccggat gccgtgattt gcctggcgac tggagccacg ccattactga cgtatcatta
    18601 tctggtagaa aaaatccatc agcagcaggt tgatgtcagc caactcacct tcgtgaagct
    18661 cgacgaatgg gtggatctgc cattaacgat gcctggcacc tgcgaaactt tcctgcaaca
    18721 gcatatcgtg cagccgctgg ggctacgtga agaccagctc atcagctttc gctccgaaga
    18781 gataaatgag acagagtgcg aacgggtaac gaacctgatt gcgcgcaaag gcggtctgga
    18841 tttatgcgtt ctcggattgg ggaaaaacgg tcatcttggg ctgaacgaac cgggagaaag
    18901 cctgcaaccg gcctgccata tcagtcaact tgatgccaga acacagcaac atgagatgtt
    18961 aaaaaccgcg ggtcgccccg tgactcgtgg gatcacctta ggcctgaagg atattctcaa
    19021 tgcccgcgaa gttttgttac tggtgactgg cgaaggaaag caggatgcga cagaacgttt
    19081 tctcacggct aaagtctcca ccgctatccc ggcttcgttt ttatggctgc acagtaactt
    19141 tatttgttta attaacacct aaccagatga aaaatttgct caaaatgttt aaattaactt
    19201 atgtaacagt catgcattat attaaataac atttgactgg gttgaacata acgccaatag
    19261 caaaaggagt tatgttcaac aatttaattt tagatataca tataacttgt tctattaata
    19321 aaccatctcg tattctgact cgccccctgg cgcagcaaag tcatgcactt atactctttt
    19381 gaatacatag aaaattgata tcaatataat gaaaatatca aatatttgca tataaatata
    19441 atcttaaagt ttagtctatt taatgttcaa tgaaatattt ctacctgtat aatctttaaa
    19501 gatgttgaac atatattcac attaaatatg attatgtact tgttacaagg ataaggttat
    19561 aaatgaataa agttacaaaa acagctattg cgggcttact tgcgcttttc gcaggtaatg
    19621 ccgctgcaac cgatggtgaa atagtttttg atggcgagat tttaaaatcc gcttgtgaaa
    19681 tcaatgactc tgataagaaa attgaagttg ctcttggtca ctataatgcc gaacagtttc
    19741 gttctgtagg cgatcgtagc ccaaaaattc cattcactat tcctttagtt aactgcccag
    19801 tgactgggtg ggagcacgat aacggcaacg ttgaagcgtc tttccgttta tggctggaaa
    19861 cgcgggataa tggtaccgtc cctaacttcc ctaacttggc aaaagtgggc tcttttgctg
    19921 gcacagcagc aacaggtgtg ggtatccgta ttgacgatgc ggaaagcgga aaccttatgc
    19981 cactaaatgc tatgggcaat gataacacag tttatcagat ccctgcggac tccgctggaa
    20041 ttgtcaatgt tgacctcatc gcttactacg tatcaaccgt tgaagcatca gaaatcaccc
    20101 caggggaagc agacgctgtc gttaacgtaa cgctggatta ccgttaatac gttacggcgt
    20161 tatctgacct ttcagataac gcccttttcc ttcctctttc tcgttgtatc aggttgaaaa
    20221 ataactatgt caaaacgaac attcgcggtg ataataacct tgttgtgtag cttctgtatc
    20281 ggccaggcgc ttgcaggggg tatcgtttta cagcgaacgc gagtgatcta tgatgccagc
    20341 cgcaaagagg ctgcgttacc tgtcgcaaac aaaggcgcag aaacgcctta tttactgcaa
    20401 tcatgggtag ataatataga tggtacaagc cgtgccccat ttattataac cccaccgcta
    20461 tttcgtcttg aagctggcga tgattcatca ctgcgaatta ttaaaacagc tgataacctg
    20521 cctgaaaata aagagtcgct gttctacatt aatgttcgtg ccattccagc aaagaaaaaa
    20581 tcagataatg ttaatgctaa cgagttgacg ctggtattta aaacacggat caaaatgttt
    20641 tatcgccccg cacacctgaa gggacgggta aacgatgcgt ggaaatcact ggaatttaaa
    20701 cgtagtgacc attcactcaa tatatataac ccaactgaat attacgtcgt atttgccgga
    20761 ctggcagtcg ataaaaccga tctcacaagc aaaattgaat atatcgcacc cggagaacat
    20821 aaacagttac cacttcctgc atctggcgga aagaacgtga aatgggctgc gatcaatgat
    20881 tatggcggca gttccgggac agaaactcgt ccactgcaat aacaaatata aaaaacacag
    20941 gtcatcaggg aatgccacaa cgacaccacc agggacataa acgcacaccg aaacagttgg
    21001 cgctcattat caaacgctgt ttgccgatgg tgctcactgg cagcggcatg ctttgcacta
    21061 ccgctaacgc cgaagagtat tatttcgacc ccattatgct ggaaaccaca aaaagtggta
    21121 tgcaaacaac cgatctgtca cgattttcaa agaaatacgc acaactacca ggaacttatc
    21181 aggttgatat ctggctgaat aaaaagaagg tttcacagaa aaaaattaca tttaccgcca
    21241 atgccgagca acttctgcag ccacagttta cggttgaaca actacgtgag ctgggtatta
    21301 aggtggatga aatcccggcg ctggctgaaa aagatgacga tagcgtgatc aactcgcttg
    21361 aacaaatcat tcccggtaca gctgctgaat ttgatttcaa tcatcagcga cttaatttga
    21421 gcattcccca aattgcactg taccgtgatg caagaggtta cgtctcccct tctcgttggg
    21481 acgatggtat accaacgctg tttaccaact actcgtttac aggttctgat aaccgttacc
    21541 gccagggcaa tcgtagccaa cgacagtacc tgaatatgca aaatggtgct aattttggcc
    21601 cctggcgatt acgcaactat tccacatgga cacgcaacga tcagacgtca agctggaaca
    21661 ctatcagtag ttatttacaa cgtgatatca aggcgttgaa atctcagttg cttctgggag
    21721 aaagcgccac cagcggcagt attttttcca gctacacctt tactggcgtg caactcgctt
    21781 ccgacgataa tatgttgcca aacagccagc gcggatttgc cccaacggta cgcggtatcg
    21841 caaacagtag tgcaatcgtg actatcaggc aaaatggtta tgtgatctat caaagcaacg
    21901 tgccagcggg tgcctttgaa attaacgatc tctacccctc ttccaacagc ggcgatttag
    21961 aagtcacgat tgaagaaagt gacggtacgc aacgtcgctt tatccagcct tattcttcat
    22021 tacccatgat gcagcgacct gggcatctaa aatatagcgc gaccgctgga cgctatcgcg
    22081 ctgatgcaaa cagtgatagc aaggaacccg aatttgctga agccacggca atatatggtt
    22141 tgaataatac ttttacgctg tatagtggcc tgctcggttc tgaagattat tatgcgctgg
    22201 ggatcggtat cggcggcaca cttggcgcac tgggcgcgtt gtcgatggat atcaacagag
    22261 ctgacaccca attcgataac cagcactctt ttcatggcta tcaatggcgt acgcagtaca
    22321 tcaaagatat cccggaaacc aacaccaata tcgctgtcag ctactatcgc tataccaacg
    22381 atggctattt tagttttgat gaagccaata cccgcaattg ggactataac agtcgccaaa
    22441 aaagtgaaat tcaattcaac atcagccaga caatatttga tggggtaagt ctgtatgcct
    22501 ccggttcaca gcaagactat tggggcaata acgagaaaaa caggaatatc tctgttgggg
    22561 tttccggcca gcaatgggga attggttaca gcctgaatta tcaatacagc cgctacactg
    22621 atcaaaataa tgaccgtgca ctctctttga atctcagtat tccgttagaa cgctggttac
    22681 cgcgtagccg ggtttcctat cagatgacca gccagaaaga tcgcccaacc caacatgaaa
    22741 tgcgtcttga tggttcactg ctggatgatg gtcgcctgag ctatagcctg gaacaaagtc
    22801 tggatgacga taacaaccat aacagtagcg tgaacgccag ttaccgttca ccttatggaa
    22861 ccttcagtgc cggatacagt tacggtaatg acagtagcca atacaattac ggcgttaccg
    22921 gcggcgtggt tatccatcct catggtgtga cgctctcgca atatctgggc aacgcttttg
    22981 cgcttattga tgctaacggg gcatctggcg tgaggataca aaactatccg gggattgcta
    23041 ctgatccctt tggctatgca gtggttcctt atctcacgac ttatcaggaa aaccgtctct
    23101 cggtagatac tacgcagctg cccgataacg tcgatcttga gcaaacaaca cagtttgtgg
    23161 tgcccaacag aggtgcaatg gtagcggcgc gtttcaacgc caatatcggt tatcgcgtac
    23221 ttgttacagt cagcgatcgc aacggtaaac cgttgccctt tggcgctctt gccagcaacg
    23281 atgagacggg gcaacaaagt atcgtcgatg agggcggcat actatatctc tctgggatat
    23341 cgagtaaatc acaaagctgg actgtacgct ggggaaatca ggcagatcaa caatgtcagt
    23401 ttgcttttag tacaccggat tcagaaccaa caacctctgt attacaaggc acagcgcagt
    23461 gccattaagg ataaaaaaat gaaaagagcg cctcttataa caggactttt gttgatatcc
    23521 acatcctgcg cttatgcctc ctcaggaggg tgtggagccg acagcactag cggtgcgaca
    23581 aattacagca gtgtggttga tgatgttacg gtgaaccaga cagataacgt gacaggacgg
    23641 gagtttacct ctgcaacgct aagtagcact aactggcaat acgcctgttc ctgctctgcg
    23701 ggtaaggcag ttaaacttgt ctatatggtc agccctgtac tcaccaccac tggacatcag
    23761 gcaggatatt acaaactcaa tgatagcctg gatattaaaa ccaccttaaa ggcgaatgac
    23821 attccaggac tcgtgactga ccaaaccgtt tctgttaaca cccgattcac acagataaaa
    23881 agcaacaccg tatattctgc tgcaacccaa acgggtgttt gccagggtga cacgtctcgt
    23941 tatggacccg ttaatattgg tgcaaatacc acctttaccc tgtatgtcac caagccattt
    24001 ctcggctcga tgaccattcc gaaaacggat attgccgtca ttaaaggcgc gtgggtcgat
    24061 ggaatgggaa gcccgtctac aggtgacttc catgatttag tcaagttatc gattcaagga
    24121 aatctcaccg ccccgcagtc gtgcaaaatt aatcagggcg atgttattaa ggttaatttt
    24181 ggattcatca atggtcagaa gtttaccacc cgcaatgcca tgccagacgg ttttactcca
    24241 gtagactttg atatcactta tgactgtggt gatacttcaa agattaaaaa ctcgttgcaa
    24301 atgcgcatcg acggtacaac tggggtagta gaccagtaca accttgtcgc cagacgaaga
    24361 agttcagaca atgcgcccga tgtcggtatt cgtattgaaa atctcggcgg cggagttgca
    24421 aatattcctt ttcagaacgg tatccttccc gttgatcctt ccgggcatgg caccgtcaat
    24481 atgcgcgcct ggccagttaa tctggtcggt ggtgagctgg aaacaggaaa atttcagggc
    24541 acagccacca ttaccgtcat cgtgcggtaa aagaagacgt tgcgttttga ttaacgcaac
    24601 gcttcggcac tcttacccct gctgctccag cgcatactta tacagcgcat ttttcttcac
    24661 gccatgaatc tctgcggcca gcgccgccgc ttttttcagc ggtagttctg cctgtagcag
    24721 cgccagcgtg cgcagggcat cggcgggtaa gtcttcttcc tgcgctttat ggccttcgac
    24781 aatcagcacc atttcgcctt tgcgacggtt ttcatcttct ttgacccacg ccagcagctc
    24841 gccaacgggc gcgccgtgaa tggtttccca ggttttggtg agctcacgcg ccagaaccac
    24901 gtagcgggat tcgcctaata ccgcaacgat atcttccagg ctgtctaaca gacggtgggt
    24961 tgattcataa aaaatcagag tgcgcggctc cgcttcaatg gcttttagcg catcacggcg
    25021 gccttttgat ttggcaggta aaaagccttc gtaacagaaa cggtcagagg gtaaacccgc
    25081 ggcgcttaac gcagtgatag cagcacacgg cccgggtagc ggcaccacgc gaatccccgc
    25141 ttcacggcag gtacgcacca gatggtagcc aggatcgtta attagcggcg ttccggcatc
    25201 ggaaaccagc gcaatgtttt gcccctcttg cagcttcgcc agcagcgttt cggctttttg
    25261 ttgttcgtta tggtcgtgca gcgcaaacaa ccgggcatta atcccaaaat gttgcagcaa
    25321 taaaccggtg tgacgagtat cctcggcggc aatcagatca acggcctgta atacctctaa
    25381 cgcacgctgg gtgatatccg ccagattgcc gattggcgtc ggtacaatgt aaagctggcc
    25441 ctgagaatta tccgccgatt ggtgttgttt cattgtgtcg tccgtattgc cgatttaata
    25501 ttgagcattg cgtaaaaaaa atatcactgg atacattatg gtaccctcaa cattttctcg
    25561 tttgaaagcc gcgcgttgtc tgcctgttgt tctggcagcc ctgattttcg ccggttgtgg
    25621 cacccatact cccgatcagt ccactgctta tatgcagggc acggcgcagg ctgattctgc
    25681 cttttatctt cagcagatgc agcaaagctc tgatgatacc aggatcaact ggcaattact
    25741 cgccattcgt gcactggtga aagaaggtaa aaccgggcag gcggtggagt tgtttaacca
    25801 actaccgcaa gaactgaacg attctcagcg tcgcgagaaa acactgctgg cggtagagat
    25861 taaactggcg cagaaagatt ttgctggcgc gcaaaatttg ctggcgaaaa tcacacctgc
    25921 cgatttagaa caaaaccaac aagcccgtta ctggcaggca aaaatcgatg ccagccaggg
    25981 gcgtccttcc attgatttac tgcgcgcgtt aattgctcag gaaccgctgc tcggcgcgaa
    26041 agaaaaacag cagaatattg atgccacctg gcaggcgctc tcctccatga ctcaggaaca
    26101 ggcgaatacg ctggtgatca acgccgacga aaatattctg caaggctggc tggatctgca
    26161 gcgcgtctgg tttgataacc gaaacgatcc cgacatgatg aaagccggga tcgccgactg
    26221 gcagaaacgt tatccgaaca atccgggcgc gaaaatgctg ccaacgcagt tggttaacgt
    26281 aaaagcgttt aaaccagcat caaccaacaa aatcgccctg ctgttgccgc tgaatggcca
    26341 ggcagcggta tttggtcgca ctattcagca aggctttgaa gcggcgaaaa atatcggcac
    26401 tcagccagtg gcggctcagg tagctgccgc acctgccgca gacgtagcag aacaacctca
    26461 gccgcaaacc gtggatggcg ttgccagccc ggcacaagcc tcggttagcg atctgaccgg
    26521 tgaacagcct gcagcccagc cggtgcctgt aagcgccccg gcgacaagca ccgcagcggt
    26581 aagcgcaccc gcaaatccat ccgcagagct gaaaatctac gatacctcat cacaaccact
    26641 tagccagatc ttaagccagg ttcagcagga tggcgcgagt attgtggtcg gtccgttgct
    26701 gaaaaataac gttgaagagt tgctgaagag caacaccccg ctgaacgtgc tggcactgaa
    26761 ccagccggag aatatcgaaa accgcgtcaa tatttgttac ttcgcgcttt cacctgaaga
    26821 cgaagcgcgc gatgcagcgc gtcatattcg tgaccagggt aaacaagcgc cgctggtgct
    26881 gatcccacgc agttcattgg gcgatcgcgt agccaatgcg tttgcgcaag agtggcagaa
    26941 actgggcggc ggcaccgttc tgcaacaaaa atttggttcc accagcgaat tacgcgcggg
    27001 tgttaacggc ggttctggta ttgctttaac gggtagcccg attactccca gagcgacaac
    27061 cgactccggc atgacgacca acaatccaac gctgcaaacc acgccaaccg atgaccagtt
    27121 caccaataat ggcggtcgtg tcgatgcggt gtacattgtg gcaacgccgg gtgaaatcgc
    27181 ttttatcaaa ccgatgatcg ccatgcgtaa cggtagccag agcggtgcaa cgctgtacgc
    27241 cagctcccgc agtgcgcaag ggaccgctgg cccggatttc cgactggaga tggaaggctt
    27301 gcagtacagc gaaatcccga tgctggcagg cggtaatctg ccgttaatgc agcaggcgct
    27361 cagcgcggtg aacaacgatt attcgctggc tcgcatgtat gcgatgggcg ttgatgcctg
    27421 gtcgttggca aatcatttct ctcaaatgcg ccaggttcag ggttttgaaa tcaacggtaa
    27481 taccggaagc ctgacggcaa acccggattg cgtgattaac aggaagttat catggctaca
    27541 gtaccaacaa ggtcaggtag tccccgccag ttaaccacca aacagaccgg cgatgcgtgg
    27601 gaagcacaag cgcgtcgctg gctggaaggc aaaggactgc ggtttatcgc cgctaacgtg
    27661 aacgagcgtg gcggcgagat cgatctgata atgcgtgaag gccggaccac cgtttttgtc
    27721 gaggtacgct atcgccgctc tgcgctttat ggcggcgcgg cagccagtgt gacccgcagc
    27781 aaacaacaca aattattaca gactgcccgc ttgtggctcg cgcgtcataa tgggagtttt
    27841 gatactgtgg attgccggtt cgatgtggta gccttcaccg ggaatgaggt tgagtggatt
    27901 aaggatgcct ttaatgacca ctcataatta aggtttaagg attagcgtgc aagaaagaat
    27961 taaagcttgc ttcactgaaa gcattcaaac tcaaattgcg gcggcagagg cgcttccgga
    28021 tgccatctcc cgtgcagcca tgacgctggt tcagtctctg ctcaatggca acaaaatcct
    28081 ctgttgtggt aatggaactt ccgctgccaa tgcacagcat tttgctgcca gcatgatcaa
    28141 ccgtttcgaa acggagcggc ccagcttacc tgccattgca ctaaatactg ataatgttgt
    28201 cttaacggcg attgccaacg atcgcttaca tgatgaagtg tatgcaaaac aggtgcgggc
    28261 gctgggtcat gcgggagatg tattgttagc catttccacg cgtggcaaca gccgcgatat
    28321 tgtaaaagcg gtagaagctg ccgttacgcg tgatatgacc attgtggcat taaccggcta
    28381 tgacggcggt gaacttgcag gtttgttagg gccacaggat gtggaaatcc gcattccttc
    28441 gcatcgtagt gctcgcattc aggaaatgca tatgctgacg gtaaattgcc tgtgcgatct
    28501 gatcgataac acgcttttcc ctcaccagga tgattaagga gaatacatga aggcattatc
    28561 gccaatcgca gtccttattt ccgcgctgct gttgcaaggt tgtgttgccg ctgccgtagt
    28621 gggtaccgct gctgtgggta ccaaagccgc aactgaccca cgcagtgtcg gcacccaggt
    28681 ggacgatggt accctggaag tgcgcgtgaa cagcgcattg tcgaaagacg aacagattaa
    28741 gaaagaagcg cgcattaatg taacggccta tcagggcaaa gtgctgctgg ttgggcagtc
    28801 accaaacgct gaactttcgg ctcgcgccaa acagattgct atgggcgtag acggtgccaa
    28861 cgaagtgtat aacgagattc gtcagggcca accgattggt ctgggcgaag cgtctaacga
    28921 tacgtggatc accaccaaag tgcgttcgca gctcttaacc agcgacctgg tgaaatcgtc
    28981 caacgtgaaa gtgaccaccg aaaacggtga agtgttcctg atggggctgg tgactgaacg
    29041 tgaagcgaaa gcggcggcag atattgccag ccgggtaagc ggcgtgaaac gggtaactac
    29101 ggcgtttacg tttattaaat agcagcccct tgtaatgcct gatgcgacgc ttgccgcgtc
    29161 ttatcaggcc tacaacgaca caaaacgtag gtcagataag gtgtttacgc cgcatccgac
    29221 tgctgttgcc tgatgcgacg cttgccgcgt cttatcaggc ctacaacgat acaaaccgta
    29281 ggtcagataa ggcgtttacg ccgcatccga catgttttcc ctcaaatcag aacaacagcg
    29341 ccagcccgcc gacaatcaca ccagacactg ccaccatcgc ccccgtcagc cataaggctt
    29401 tcgccgggaa cgctttgcgc agcataatca gtgacggcaa actcaccgcc gggagcgtca
    29461 tcaacaatgc cagcgccgga gcagttccca tacctgccag catcatcgtt tgtacaatcg
    29521 gaatttctgc tgccgtggga atgacaaaca agcatcctgc caccgccatc gccaccaccc
    29581 acatcaggct gttatcgaca gtaccatcgg catgggggaa taaccagacg cgagcggcac
    29641 ccaacaccag tactgcaagg atgtaaaccg ggatcgtact ccagaaaagc gtccatagcg
    29701 ccctgcccca gcggctaaaa aaaccgccct gtgcttccgg tatgtcaatt tcgaccggtg
    29761 cctgcgtttg cggtgtttca cgcacccatt tttgcaccag cgtcgcaatc agcaacacca
    29821 tcaccagccc ggccaccaga cgaatcgccg caaaacccca gccgaggaca aagcccataa
    29881 acaccagcgt cgccgggttt aacaccggat tgcccatcca gaatgccagc gcaccgccca
    29941 tcgacacctg ttggcgacgc attcccgccg cgaccggagc cgcacagcag gtacacatca
    30001 tgcccggcaa cgaaaacagc gttcccagca gcgtgccgcg aaagcgcgat tgcccaagcg
    30061 tacgcaacaa ccagtcacgc gggatcagca cctgaatcaa cgaaccgagg atcaccccca
    30121 gcaccgccgc tttccatacc gcgaggaaat agatcatcgc gtaatccaac gccgcctgcc
    30181 atgggttagc atccgcctgc gcaaggatag atttaccgat actgtgggtt tcggcagcag
    30241 taaacgcttt gccgtagtaa ggttcccatt tcacatacca gagaccggca atgacaacga
    30301 gaaagaaaag cgcgggtttc caccactgaa tgggcgttgc cgcctgagat gaagactgac
    30361 cagtcatagc attccccagg aataagttat gataattgag cgcgtgaata ttacgctcac
    30421 tatcaattct tgggaataat tattcagctc ttttgcgtaa ttctgaagag cttaaaatcg
    30481 tcacgccttc atgctcaggc cgcatcgact ctgccagcat gacccgcgcc acgtctctcg
    30541 catcaatgga tttccagtta cctggtaaca aacggaacag cggcgcaaaa agcgtttcgt
    30601 tcatccgctg tttgctacga tcgcccagta acatcgacgg gcgagcaatg gtcaatttcg
    30661 gccagttctg ggcaattaat gcttcttcca tctccccttt gacgcggtta tagaaaaacg
    30721 gcgagtgggc attggcaccc atagcactga ccaccagcat atgctgcgcg cccagccgcc
    30781 gcccggttaa tgcggtatcc actaccagcg tgtaatcggc atgaataaac gcctctttgc
    30841 tccccgcttc tcgccgcgtg gtgccgagac aacaaaacac aatgtcgatg ggatcggtga
    30901 cctgcgccag cgcgtcagtc agttgcggat catggggatt gaacactcct ggcatatctc
    30961 ccaacggacg tcgcgtcggt gcagcaatag cgttaacttt cggttcgtta atcaacatcc
    31021 gcagcaggtg accgcccacc agccccgttg cgcctgtaat cagtacctga ctcatcttcg
    31081 ctcctttaca gaattgtctg ccttgcgctc cccggctcat gcaccaggct taatagacca
    31141 tgaggtaatt atccccgatt gtgagaaatt cgcctcatcc aatgcaacaa cgcggaggaa
    31201 gcatgagtaa gaaaattgcc gttttaatca ctgatgaatt tgaggattca gaatttactt
    31261 cacccgcaga cgaattccgt aaagccggac acgaagtgat taccattgaa aaacaagcgg
    31321 gtaaaacggt gaaaggcaaa aaaggagaag ccagcgtgac catcgataaa tccatcgatg
    31381 aagtgacgcc agcggagttt gatgccctgt tgctaccggg cggtcattca ccggattatc
    31441 tgcgtggcga caaccgtttt gtcaccttta cccgtgattt tgtgaatagc ggcaaacctg
    31501 ttttcgccat ctgtcacggt ccgcagttgc tgatcagcgc cgatgtgatt cgggggcgca
    31561 aactgaccgc agttaaaccg atcattattg atgttaaaaa tgcgggcgcg gaattttacg
    31621 atcaggaagt cgtggttgat aaagatcagc tggttaccag ccggacaccg gacgatctgc
    31681 cagcgtttaa ccgcgaggcg ttacgcctgc tcggtgcctg agtcgcgtag ccaaatcatt
    31741 tttttaccaa agcccagcgt gttgtcggtg aatttgattt catcgagccg gatttcccac
    31801 accggggccg acagcattct ggcaaccgga aagcgacgat tgtacgcctt gcgcgcgagg
    31861 tcgctttcct caccttccag cctgcggatc tcacctttaa actgcacacc gcgaattaac
    31921 gctaccgttt tcggctgacc gtttaccgtt ccggcaactg ccgcctgcgg cccgctcatc
    31981 tgcgcgtggc gcgttttttc ttccgtcaaa atgtagaagg cgactttctg cgcatcaaaa
    32041 agataaaagg cattagcgca ccagagttcc ccttcctgct gcacacacca ggtgacaaca
    32101 tgttgttttg ccagccaacg gctgatggcg atgagtgttt ccattgctgt tctcttttat
    32161 actgtgggcc tgaccttaac atgcgatgca gacgatgaca ccctggtttc tttacttgat
    32221 ccgtaccgcc gacaataagc tttataccgg gatcaccacg gatgtcgaac gccgctatca
    32281 gcagcaccaa agcggcaaag gggcgaaagc actgcgcggg aaaggagaac tgacgctggc
    32341 gttttctgct ccagtgggcg atcgttcgct ggcgttacgg gcggaatatc gcgttaagca
    32401 actgaccaaa cggcaaaaag agcgtctggt agcggaaggc gcagggtttg cagagctgtt
    32461 aagcagtctg caaaccccgg agattaaaag cgattgaaat gctcgtgata ctcaaccagg
    32521 ccggtaacgc cattcagcgc gtcatccgct aaacgatgta cctggaaggc gctttcagtg
    32581 cccggccagc ggcaacgcag atcgtgatgc gccgccagtt caaagccgaa acgactgtac
    32641 agcgccggat cgcccagcgt caccactgcg gcatagccga actcattaag cgaatcgagt
    32701 ccttcataga ccagttggcg tgccagcccc tgtccacggt atttttcatc aaccgccaac
    32761 ggtgccatgc cgacccattg caggtcttcg ccctgcacat caaccggact aaatgccaca
    32821 tagccaatga cctgaccttc gtcatctgtc gccaccagcc ccagcgtcag aaagccatct
    32881 tcacgcagat cgtgaaccag cttcgcttcc gcatcacttt cgaatgagcg acgcagcagg
    32941 gcatcaatac ccggcgcatc aatgggaatt tctactcgaa ttagcatggt tcacctaccg
    33001 atgtctgttt ggtttcaggc gcggttttca ttcccgcctc aacaaaatcc gccagttgca
    33061 gcagcatcat gcgtaaggct ttcggcattt gctccagttc aatggcgtcc atcaggtttt
    33121 tcacatacag ccccagctcc gtatcgcctt caatcaccag ccgacgctgg aagaagagcg
    33181 tatccggatc ttgtttacgc gccgcaatca tcagcagatc gctggcgtcg gcactaaaac
    33241 tcacatcagc ttgcgcgttc tggctgacca ccagtttgcc attcaccacc gaggtaaacc
    33301 attgcaggtc aatatcacgc acatgaatac ttaaccagcg gccttcaaga aactccagct
    33361 cgccatcatc cagcgcctga cggaattgcc agcttaagac ctgctcaaga acctggcgtt
    33421 ttagcgcaaa tggcgtcagt tttaccggta cactcaacag agatggcccc aaatgcacaa
    33481 tacgggaacg cagtttatcc aacacgagtt ttactccctg tttcaacaat catcctattt
    33541 tgccatatca gaaaaataac atagcggtat aaatcaacaa ttccatatga aattgctgct
    33601 accaccaata caactttaac tgccttaaat caaaaattgt cgcagcaagg ttaactaaaa
    33661 tcccagttcg ttaacatttt tgcgttttga tagcgcaacc ttcaggaaaa attatggagc
    33721 tgctctgccc tgccggaaat ctcccggcgc ttaaggcggc catcgaaaac ggcgcagatg
    33781 ctgtttatat cgggctaaaa gatgatacta atgcccgtca cttcgccggc cttaacttta
    33841 ccgagaaaaa attgcaggaa gcggtgagtt ttgtccatca acatcgccgc aaacttcaca
    33901 tcgcgattaa cacttttgcg catccggacg gttacgcccg ttggcagcgc gccgtggata
    33961 tggcggcgca gctgggtgcc gacgcgctga tcctcgccga cctcgccatg ctggagtacg
    34021 ccgccgagcg ttacccgcat attgagcgtc atgtgtcagt gcaggcttcg gcgaccaatg
    34081 aagaggcgat taacttttat catcgccatt ttgacgttgc tcgcgtggtg ctgccgcgcg
    34141 tgttgtcgat tcatcaggtg aaacagctgg cacgggttac tcctgtcccg ctggaagtgt
    34201 ttgctttcgg cagcctgtgc attatgtcgg aaggtcgttg ctatctgtcg tcgtatctga
    34261 cgggtgagtc gcccaacacc gtgggagcgt gttctccggc ccgtttcgtg cgctggcaac
    34321 aaacgccgca ggggctggaa tcccgcctga acgaagtgct gatcgaccgt tatcaggacg
    34381 gcgaaaacgc aggttatccg acgctgtgta aagggcgtta tctggtggac ggcgagcgct
    34441 atcacgcgct ggaagaacca accagtctca ataccctgga actgctgccg gagttaatgg
    34501 ctgcgaatat tgcttcggtg aaaattgaag gccgccagcg tagcccggcg tatgtcagcc
    34561 aggtggcgaa agtctggcgt caggctatcg accgttgtaa ggccgatccg caaaactttg
    34621 taccgcaaag cgcgtggatg gagacgctcg ggtcgatgtc cgaaggcacg caaaccactc
    34681 ttggcgcata tcaccgtaaa tggcagtgag aaaagcaatg aaatattcct tagggccagt
    34741 gctgtggtac tggccaaaag agacgctgga agaattttat cagcaggccg ccaccagcag
    34801 cgccgacgtg atttatcttg gtgaagcggt atgcagcaag cgtcgggcaa ccaaagttgg
    34861 cgactggctg gagatggcaa aatcgctcgc cgggagtggt aagcagattg tgctctccac
    34921 gctggcgctg gtgcaggcat catctgaact gggcgaactg aaacgctatg ttgagaacgg
    34981 tgagtttctg attgaagcca gcgatctcgg cgtggtgaat atgtgcgctg aacgcaaact
    35041 gccgttcgtc gccgggcacg cgctgaactg ctacaacgcg gtgacactga aaatattgct
    35101 caaacagggc atgatgcgct ggtgtatgcc agtggagctt tcccgcgact ggctggtgaa
    35161 tctgcttaat cagtgcgatg agctgggcat tcgtaaccag tttgaagtgg aagtcctgag
    35221 ctacggtcat ctgccgctgg cctactccgc ccgctgcttt accgcgcgtt cggaagaccg
    35281 cccgaaagat gagtgtgaaa cctgctgcat taagtatccg aacgggcgca acgtgctgtc
    35341 gcaggaaaac caacaagtgt ttgtactcaa tggcattcag accatgagcg gctacgttta
    35401 caacctcggt aacgagctgg catccatgca gggcctggtt gatgtggtcc gcctgtcacc
    35461 gcagggtact gacactttcg cgatgctcga cgccttccgc gctaatgaaa atggcgctgc
    35521 accactgccg ttgacggcga acagcgactg taacggctac tggcggcggc tggccgggct
    35581 ggagctgcaa gcctaagtaa atatgctcgt catacttcaa gttgcatgtg ctgcatctgc
    35641 attcgttcac cccagtcact tacttatgta agctcctggg gattcactct cttgtcgcct
    35701 tcctgcaact cgaattattt agagcacagc tcactttgtt aacaacttta actactcttt
    35761 aatgcagtat taaagattaa tcggtaacaa agtgagctgt tatgactgat aaaaccattg
    35821 cgttttcgct actcgatctg gcccccattc ccgaaggttc ttcagcgcga gaagcattct
    35881 cccactctct cgatctcgcc cgtctggctg aaaagcgcgg ctatcatcgc tactggctgg
    35941 cggaacacca caatatgacc ggcattgcca gtgctgcaac atcggtattg attggctatc
    36001 tggcggcgaa taccaccacg ctgcatctgg ggtctggcgg cgtgatgttg cctaaccact
    36061 caccgttggt cattgccgaa cagttcggca cgctcaatac gctctatccg gggcgaatcg
    36121 atttggggct gggtcgtgct ccgggtagcg atcagcgaac catgatggcg ctacgtcgtc
    36181 atatgagcgg cgatattgat aatttccccc gcgatgtcgc ggagctggtg gactggtttg
    36241 acgcccgcga tcccaatccg aatgtgcgcc cggtaccagg ctatggcgaa aaaatccccg
    36301 tgtggttgtt aggctccagc ctttacagcg cgcaactggc ggcgcagctt ggtctgccgt
    36361 ttgcgtttgc ctcacacttc gcgccggata tgttgttcca ggcgctgcat ctttatcgca
    36421 gcaacttcaa accgtcggct cgactggaaa aaccatacgc gatggtgtgc atcaatatta
    36481 tcgccgccga tagcaaccgc gacgctgagt ttctgtttac ctcaatgcag caagcctttg
    36541 tgaagttgcg ccgtggcgaa accgggcaac tgccgccacc gattcaaaat atggatcagt
    36601 tctggtcgcc gtccgaacag tatggtgtgc agcaggcgct gagtatgtcg ttggtaggtg
    36661 ataaagcgaa agtgcgtcat ggcttgcagt cgatcctgcg cgaaaccgac gccgatgaga
    36721 ttatggtcaa cgggcagatt ttcgaccacc aggcgcggct gcattcgttt gagctggcga
    36781 tggatgttaa ggaagagttg ttgggatagt gtgtcttaac gcgggaagcc ttatccgagc
    36841 tggcaacgct gtcctacgta gacctgataa gcgaagcgca tcaggcattg tgtaggcagc
    36901 agaaatgtcg gataaggcac cgctgattac tgatacaccg gcagtaaatt aaagctcgat
    36961 aaaatatgga ccagtgcgtt gccaacgcca aacaccagaa tcagcgtaat catcggcttg
    37021 ccgccccaga cgcggaattt cgggctgcca aagcgtttac gcgatgcgcg ggctaacagc
    37081 gccggaacaa ttgccgccca gatggtagct gctaaaccag cataaccaat ggcgtacagg
    37141 aatccgttcg ggaacagtag accacccaca acaggcgggg caaaggtcag caatgccgtt
    37201 ttcaagcgcc ccagggctga gtcatcgaaa ccaaacagat ctgccagata gtcaaacaaa
    37261 cccagcgtta cgccaaggaa cgaactcgct accgcaaagt ttgagaacac gaccagcaga
    37321 aggtccaggc tacggctgtt cagcacgccg cttaatgcct gtaccagcac atcaatatta
    37381 ccgcccttct ctgcaatacc gataaactcc ggacgcggga tgttacccat cgtcgccagc
    37441 aaccagatgg tatacagcgc cagtgccatc agcgtaccgt acaccaggca tttcacgatg
    37501 gttttcggat ctttgccgta atacttcatc aggcttggca cgttaccgtg ataaccaaac
    37561 gacgccaggc agaacggcaa tgtcatcagc agatacggcg cataagatgc attgctttct
    37621 gcaacgttga acaatgtcgc aggctgcaca tgccccaaca ggctgccaaa ggtgaggaag
    37681 aaggtaatga ctttcgcccc cagcacaatc gccgtcatgc ggctgacggc cttagtactc
    37741 aaccacacca caaacgctac cagcagtgca aaaccaaaac ccgccgcccg tgccgggacg
    37801 tttagcgaca tctctgcgaa ggtgtgatgc agaatcgaac cactggcaga aatataggca
    37861 taggtcagga tatagagcac aaaggcaatg gaaataccgt tgaccacgtt ccagcctttg
    37921 cccagcaaat ctttggtgat ggtgtcaaaa ctcgaaccga ttcggtaatt caggttagct
    37981 tccagaatca tcaagccgga atgcagcata cagaaccagg taaagatcag cgccgccatt
    38041 gaccagaaaa accacgcccc ggacatgacc actggcagag aaaacatccc tgcgccaata
    38101 atggtgccgc cgataatcac cacgccgcca agcagcgacg gtgacgtttg ggtggtggtt
    38161 agtgttgcca tgagggcttc tctccagtga aaaatagtgc gactgcgttg ttatgcattg
    38221 cactgtacca gtacacgagt acaaaagaca gaaaaaaagc cccgatggta aaaatcgggg
    38281 ctgtatatat tattttacag attgtgttcg ctgttcagcg atgattacgc atcaccaccg
    38341 aaacgacgac gaccggtaga gtcatcacga cgcggagcgc ggccttcacg acgttcgccg
    38401 ctaaaacgac gaccatcacc acggccacct tcacggcgtt caccgctgaa gttacgaccg
    38461 ccttcacgac gttcgccacc gaaaccacga ccaccgccac gacgctcacc gccagtatgc
    38521 ggctgtgcat cgcccagtaa ctgcatgttc atcggcttgt tgagaatgcg agtgcgcgta
    38581 aagtgttgca gcacttcacc cggcatacct ttcggcagtt cgatggtgga gtgagaagca
    38641 aacagcttga tgttaccaat gtaacggctg ctgatgtcgc cttcgttagc aatcgcacca
    38701 acgatatgac gaacttcaac accatcatcg cggcccactt caatgcggta cagctgcata
    38761 tcgccaacat cacgacgttc acgacgcgga cgatcttcac ggtcaccacg cgggccacgg
    38821 tcgttacgat cgcgcggacc acggtcatca cggtcacgga attcacgttt cggacgcatc
    38881 ggcgcatctg gcggtacgat cagagtacgt tcaccctgtg ccattttcag cagtgccgca
    38941 gccagagttt cgagatccag ctcttcacct tctgcagtcg gctgaatttt gctcagcagt
    39001 gcgcggtatt gatccagatc gctgctttcc agctgctgct gtactttagc ggcgaatttt
    39061 tccagacggc gtttgcccag cagttctgcg ttcggcagtt ctacttccgg aatagtcagc
    39121 ttcatagtac gttcaatgtt gcgcagcaga cgacgctcgc ggttctcaac gaacagcagc
    39181 gcgcggccag cacgacccgc acgaccggta cgaccgatac ggtgaacgta agactcagaa
    39241 tccatcggga tatcgtagtt aactaccagg ctgatacgct caacgtccag gccacgggct
    39301 gcaacgtcgg tcgcaatcag gatgtccaga cgaccatctt tcaggcgttc cagtgtctgt
    39361 tcacgaagcg cctggttcat gtcaccgttc agcgcggcgc tgttgtagcc gttacgctca
    39421 agagcttcag ccacttccag agtcgcgttt ttggtacgaa cgaagataat cgccgcatca
    39481 aaatcttccg cttccaggaa acgtaccagt gcttcgtttt tgcgcatacc ccagacagtc
    39541 cagtagctct ggctgatgtc aggacgggta gtcacgctgg actgaatgcg cacttcctgc
    39601 ggctctttca taaagcggcg ggtaatgcga cgaatcgctt ccggcatggt tgcagagaac
    39661 agagcggtct gatgaccttc cgggatctgc gccataatgg tttcaacgtc ttcgatgaag
    39721 cccatgcgca gcatttcgtc agcttcatcc agaaccagac cgctcagttt agagaggtcc
    39781 agagtgccac gtttcaggtg gtccagcaga cggcccggag taccgacaac gatctgcggc
    39841 ccctgacgca gggcgcgtaa ttgcacgtca taacgctggc cgccgtacag agcaaccaca
    39901 tttacgccgc gcatgtgttt agagaaatcc gtcattgctt cagcaacctg taccgccagt
    39961 tcgcgggtcg gtgccagcac cagaatctgt ggtgctttca gctcaggatc aagattctgc
    40021 aacagaggta aagagaatgc tgcagttttt ccgctccccg tctgggccat acccagaacg
    40081 tcgcggccat tcagcagatg tggaatacac tctgcctgaa ttggagatgg tttttcgtaa
    40141 cccagatcgt taagggcttc aaggatagga gccttcaggc ccagatctgc aaaagtggtt
    40201 tcgaattcag ccatgtagta cgtgtgcctc aaaattaatg gcggccagtc tacataactc
    40261 atcatgaaat tgatcagcaa ttttcattga aaagtgtgaa ccggctcaaa gtaggtgtat
    40321 taacgaacaa caacgccctc acccgttaag gtgatggcaa tcaaaaaaga ttacgggctg
    40381 atgtgtacgt cagctattgc tggtccgatt ctgccaggtc atcttggtcc tggcccagga
    40441 gcgataattc caacaatgcg tatcggtgct caacaaagtt atgaacgttg ttggcaaccg
    40501 ccagtttgaa cagtgccgtg gcgctgtcca aatcccccag acttaggtag tacttaccta
    40561 aatagaagtt ggtttcactg agatgctcag cgagcgaggt gttatccgtt gcgtccgcct
    40621 tgagcctttc cattaacgtt tgttcgctaa tgttgcccag gtagaactcg acaatgttcc
    40681 atccccactg ttccttatcc gatttttcga agtgctgttt caacacttct ttagcctgct
    40741 tctcatcgag cttctgctcg gcgagataaa gccacagact acggaaagga tcattgggat
    40801 cgtcttgata aaacgccagc agatcatctt gcgctaactt gtcacgaccg ccgtaatata
    40861 atgcgatccc gcgattcaag tgcgcgtagt tgtaagttgg atcaagctca agtacagaat
    40921 caaacgcttc ataggcagca tcaaaattgc ctgcctgcgt taaatatatg cctaagtaat
    40981 tgaatacttc aggcatatcc ggtcggattg ccagcgcttg cgaaaaatcg ttacgcgcta
    41041 atgccctcag accgagacta tcatacaaca ctccgcgctc atataaaagc tgtgcgcgtt
    41101 cgtcatcggt taaagcccga ctggcaagga tttgttccat acgtgccaga atcacttcct
    41161 gctgtaaagt cggttgcaat ggtaccgcga ggacttcact tttacgccag gaagtattac
    41221 tgcatcctgc aagcgtaagt gctgtcgcaa cgaaacacca gcgcaaaaaa ggcttcattt
    41281 cccactcccg aagaccacgg ttgaatgaac gtcctgttcc cggttgctaa caaggcgtcc
    41341 tgcccggtta aaagcccccc gccgcagcgg agggcaaatg gcaaccttac tcgccctgtt
    41401 cagcagccgg agcttccggt gctgcagcag gttgagactg ctcagtcgct tctttaatgc
    41461 tcagacggat acggccctgg cgatcaactt ccagaacttt caccggtact tcctgaccca
    41521 tctgcaggta atcggtcact ttctcaacgc gtttgtcagc gatttgagag atgtggacca
    41581 gaccttcttt accgccgccg atggcaacaa atgcgccaaa gtcaacgata cgggtcactt
    41641 taccagtgta gacgcggccc acttcgattt ctgcagtgat ctcttcgata cgacgaatag
    41701 catgtttcgc tttctcgccg tcggtcgctg cgatcttcac agtaccgtca tcttcgattt
    41761 cgatggtagt gccagtttct tcggtcaggg cacggataac agagccgcct ttaccgataa
    41821 cgtctttgat cttatccggg ttgatcttga tggtatggat acgcggtgcg aactcagaga
    41881 tatcgccacg cggcgcgttg atcgcctgtt ccattacgcc caggatatgc agacgcgcac
    41941 ctttagcctg gttcagcgca acctgcatga tctctttggt gataccttca attttgatat
    42001 ccatctgcag tgcagagata ccgtcgcggg aacctgcaac tttgaagtcc atatcgccca
    42061 ggtgatcttc gtcgcccaaa atgtcagaca gtacaacgta gttgtcgcct tctttcacca
    42121 gacccattgc gatacccgca acggcagctt tgattggcac acctgcgtcc atcagcgcca
    42181 gagacgcgcc gcacacggaa gccatagaag aagaaccgtt ggattcggtg atttcagaca
    42241 ctacacgtac ggtgtacggg aatttgtcca tatccggcat gactgccagc acgccgcgct
    42301 tcgccagacg accgtgacca atttcacgac gcttcggaga accgaccatg ccggtttcgc
    42361 ctacggagta cggagggaag ttgtagtgga acaggaaggt atcggtacgt tcgcccatca
    42421 gttcatcaag aacctgcgcg tcacgagcgg tacccagcgt tgcagtaacc agtgcctgcg
    42481 tttcaccgcg ggtgaacagc gcagaaccgt gagtacgcgg cagcacgcca gtacgcacat
    42541 ccagaccacg gatcatatct ttttcacgac cgtcgatacg cggttcgcct gccagtacgc
    42601 ggctacgaac aacgtttttc tcgatagcgt gcagaatttc acccagttcg ttttcgtcca
    42661 gggtttcgtc ttcagcaagc agcgtcgcga tggtttcaga tttgatgaca tcaacctgcg
    42721 cataacgctc ttgtttgtcg gtgatgcggt aagcatcgct caggcgagct tcagccagtg
    42781 cagcaacgcg cgcgttcagc gcttcgttta ccggctccgg ctgccagtcc caacgtggtt
    42841 taccggcttc tttcaccagt tcattgatgt tctgaataac aacctgctgt tgttcatgac
    42901 cgaacactac tgcgcccagc atctggtctt cgctcagcag ttcagcttca gattcaacca
    42961 tcagaacagc ggcttcagta ccggcaacaa ccagatccag tttgctctct ttcagctcgt
    43021 cctgagtcgg gttcagtacg tactggtcat tgatgtaacc tacgcgagca gcaccaatcg
    43081 gaccattgaa cggaatacca gacagagaca gtgctgcgga agcaccaatc atcgcgacga
    43141 tatccgggtt aacttgcggg ttaacagaaa ccacggtggc gataacctga acttcgttga
    43201 cgaagccttc cgggaacagc gggcgaatcg ggcggtcaat cagacgcgcg atcagggttt
    43261 cgccttcgct tgggcggcct tcacgacgga agaagctacc cgggatacga ccagcagcgt
    43321 aggtacgctc ctgatagtta acggtcagtg ggaagaagtc ctgacctggt ttggcttttt
    43381 tctggccaac aacggtaaca aataccgcgg tgtcatccat gctaaccata acagcggcag
    43441 tagcctgacg agccatcatg ccggtttcca gagtcacggt gtgttggccg tactggaatt
    43501 tacgaacgat cggattaagc aatgtaatat cctttctctt tcttagacag taccttacgg
    43561 cactggtgtt aatacccgat cttctgcgca tcctcgcgac taatgacaac cctaacccag
    43621 ctctatgtgg gtaaagcctc tcattagccg cgcgaacctc tgcaacggaa gatcattcat
    43681 agcaacaata cattagtttc cagtgaattg ctgccgtcag cttgaaaaaa ggggccactc
    43741 aggccccctt ttctgaaact cgcaagaatt agcgacgcag acccaggcgc tcgatgagct
    43801 gggtgtaacg tgctacgtct ttacgtttca ggtagtcgag cagtttacga cgctgagaaa
    43861 ccatgcgcag cagaccacga cggctgtggt gatctttttt gtgctctgca aagtggccct
    43921 gcaggtggtt gatctgtgca gtcagcagtg ctacctgaac ttcggtagaa ccggtgtcgt
    43981 ttgcgtcacg accaaactca gaaacgattt tagctgttgc ttcagtactt agagacattt
    44041 taaaactcca aagtatatag aatgaaagga cgccgatctc taattcagcg atcccagtgt
    44101 acgttacgca aagtgttaaa caatttacgc gacgttaagc ggcagtattc tactcgtagc
    44161 gacctgttat cgcaagacgg ttaacattac gccgggtatt caaccaccag gcgacgaggc
    44221 gcaacgcggc cttcatcgtc aatttcgccc ataccgataa atttgccgtt ctcaccttct
    44281 gtaacgcgaa ccagtccttc cagcggcgca ccggatgtac gaactgggtt accatttttg
    44341 aagtaaacag aagacgttaa cggaagattc accaccgggt agtccgaagc tggactgtcc
    44401 attggcatca gtaatggatc aagtaactcc gcagccggaa tatcctgctg ttcggcttgt
    44461 tcgaccagtt cacgcagatg ttccagggtc accatccgtt caaccggata tttacttacc
    44521 gccagacgac gcaggtaaat aacatgcgcg ccacaaccga gtttttcacc caggtcatca
    44581 atgatggtgc ggatataagt gccttttgag cagtgaattt ccagctccag ctcattgcct
    44641 tcgtggcgaa taaacagcaa ttcataaacg gtaatcgggc gcgcttcacg cggtacttca
    44701 atgccctgac gcgcatattc gtacagtttt ttaccctgat atttcagtgc tgaatacatc
    44761 gaagggatct gttcgatatc gccacggaaa gtatccagcg ccgccgccag ttgctctgcg
    44821 ctaaaggtta ccggacgttc ttcaacgatt tgtccgtcgg catcagaagt atcggtacgc
    44881 tgtccaagac gcgcaatgac ccgatagcgt ttgtcggagt ccagcagata ctgggaaaac
    44941 ttcgtcgctt ccccgaggca aattggcaac atgccagttg ccagcgggtc cagcgcaccg
    45001 gtatgcccgg cacggttggc gttataaatc cgtttcactt tttgcagcgc atcattgctg
    45061 gacataccct gcggtttatc cagcaacaaa acgccgttaa tgtcgcgacc gcgacgacga
    45121 ggacgactca ttagtcctcc ttgctgtcgt ccgggttaac acgacgttct tcgtcatgtt
    45181 tgaccacgct ggtcaccagg tttgacatgc gcatcccttc aaccagagag ttgtcgtaga
    45241 agaaggtcag ttccggcacg atacgcaggc gcatcgcttt ccccagcagg ctgcggatga
    45301 aaccagaagc ttcttgcaac gctttgatgc ccgctttaac cgcgtcttca tctttgtcgt
    45361 tgaggaacgt cacatatact ttggcatacg ccaggtcgcg agacatttcg acaccggaaa
    45421 cggtggtcat catgcccagg cgaggatctt taatttcacg ctgcaggatg agagcgatct
    45481 ctttttgcat ttcctgcgct acgcgctgcg ggcgaccaaa ttctttcgcc ataataaatt
    45541 ctcctgacaa aaaaggggct gttagcccct ttttaaaatt aatttcaggt ggaagggctg
    45601 ttcacgttgg cctgataaga cgcgccagcg tcgcatcagg caatccatgc aggatgtggc
    45661 gtaaacgcct tatcccgcat ggaaccctaa aaaccttaag cgatggtacg ttggatttcg
    45721 atgatttcga atacttcgat cacatcgcca gtgcggacgt cgttgtagtt cttaacgccg
    45781 ataccacatt ccataccgtt acggacttcg ttaacgtcat ctttgaagcg gcgcagggac
    45841 tccagctcgc cttcgtagat aaccacgttg tcacgcagaa cgcggatcgg gttgtgacgt
    45901 ttaaccacac cttcggtaac catacagcct gcgatggcac caaatttcgg tgatttgaac
    45961 acgtcacgaa cttccgccag accgataatc tgctgtttca gttccggaga cagcataccg
    46021 ctcatcgccg ctttcacttc gtcaatcagg ttatagatga cggagtagta acgcagatcc
    46081 aggctttccg cttcaatcac tttacgtgca gaggcatcag cacgtacgtt gaagccaacc
    46141 aggatggcgt tggacgccgc agccagggtg gcgtcggttt cggtgatacc acctacgcca
    46201 gaaccgatga tcttcacttt aacttcgtca gtagacagtt tcagcaagga gtcggagatc
    46261 gcttccacag aaccctgtac gtctgccttc aggacgatgt tcacttcgtg aacttcgcct
    46321 tcggtcatgt tggcgaacat gttctcgagt ttagatttct gctgacgcgc cagtttaact
    46381 tcgcggaatt taccctgacg atacagtgca acttcacgcg ctttcttctc gtcacgtaca
    46441 acggtaactt catcacccgc agccggtacg ccggacaggc cgaggatttc caccggaatg
    46501 gacggacccg cttccagcac ttcctgaccc agttcgttac gcatcgcacg aacacgaccg
    46561 tattcgaagc cacacagaac gatatcgccc ttgtgcagag taccttcacg taccagaacg
    46621 gtagcaaccg gaccacgacc tttatcgagg aaggattcga taaccgcacc gctcgccata
    46681 cctttacgta ccgctttcag ctccagaact tccgcctgca gcaggatagc gtccagcagt
    46741 tcatcgatac cagtacccgc tttcgcagac acatgtacga actggctttc accgccccac
    46801 tcttccggca ggatgccgta ctgggagagt tcgttcttaa cgcgatccgg atcggcttct
    46861 ggtttatcga tcttgttcac tgcaacgacc accggaacct gcgccgcttt cgcgtgctgg
    46921 attgcttcga tcgtctgcgg cattacgccg tcgtcggcag caacaaccag gactacgatg
    46981 tccgttgcct gcgcaccacg agcacgcatt gaagtaaacg cggcgtgccc tggggtgtcc
    47041 aggaaggtga tcatgccgtt ttcagtttca acgtggtatg caccaatgtg ctgggtaatg
    47101 ccgcccgctt cgccagaggc cactttcgtt gaacgaatgt agtccagcag agaggtctta
    47161 ccgtggtcaa cgtgacccat gatggtcaca accggcgcgc gcggttcagc cgcagcaccc
    47221 gtgtcacggt cgctcattac cgcctcttcc agctcgtttt cacgacgcag gataacttta
    47281 tggcccatct cttcagcaac cagctgtgcg gtttcctgat cgataacctg gttgatggtt
    47341 gccattgcgc ccagtttcat catcgctttg atgacctgag agcctttaac cgccatcttg
    47401 ttcgccagtt cgccaacggt gatagtttcg ccgatcacaa cgtcacggtt aacggcctga
    47461 gcaggcttct ggaagccttg ctgcagggaa gaacctttac gttttccgcc tttaccgcca
    47521 cgtactgctg cgcgtgcttc ttcacgatca gcttttgatt cagcgtgttt gttacctttc
    47581 ttcggacgcg ctgctttcgc gttacgacca cggccacggc cgccttcgac ttcacgatcg
    47641 ctttcgtctt cggcctggcg agcatgttga gaagtagtga cgtgataatc gctggaatct
    47701 tcagtcggtt ccgcgttatc agtccatttg ttttcttccg ccatacgacg tgcttcttca
    47761 gcaacgcgac gtgcttcttc ttcgagttta cgacgcgctt cttcttcagc tttacgcttg
    47821 agctctgcag cttcctgctc acggcgggct ttttcagcct gggcgttttt agtcatatcg
    47881 tcttgttgat tgctcacttt gtctttttcc gcagcttcac gtttcgcttg ttcagcagct
    47941 tcacgcttag cttgttctgc ggcctcacgt tcagcttttt gttgcgcctc gcgtttagcc
    48001 gattcttctg cctcacgacg ggcttgctct tccgcttcac gctgcgcttg ctcttccgct
    48061 gcaaggcgtt cagcctcttg cggatcgcgt ttcacaaagg tgcgtttctt gcggacttcg
    48121 atttgtaccg atttgctttt tccaccggta ccaggaatgt taagggtgct gcgtgtttta
    48181 cgctgcagcg tcaatttgtc cgggcctgaa tttttctgat tcaggtggtc aatcaaagtc
    48241 tgtttctctt gtgcagacac agagtcgtca gcagacttcc ggatacctgc atcagcaaat
    48301 tgctgtacca ggcgttccac ggaggtctgt cgctctgcgg ccagcgtttt aatcgttaca
    48361 tctgtcatgc tgttccttcc tgctacagtt tattacgctt cgtcaccgaa ccagcaaata
    48421 ttacgggcag ccataatcag tgctccggct ttttcgtcgg tcaacccttc gatatcagcc
    48481 agatcatcaa tgccctgttc ggcgagatct tccagcgtac aaacgccacg ggcggccagt
    48541 ttgaatgcca aatcacgatc taccccttca aggttcagca gatcgtcagc cggtttgtta
    48601 tcaccgaggc tttcttcctg ggcctgtgca atggtggcca gtgcattttt agcacgctcg
    48661 cgcagtgctt caacggtcgg ctcatcaagg ccttcgattt ccaacagctc tttcatcggc
    48721 acataggcca gttcttccag cgtcgagaag ccttcttcta ccagaacagt cgcgaagtct
    48781 tcgtcgatgt cgagatattt ggtgaaggtg tcgatcgctg cgtgcgcttc cgcctgatgc
    48841 ttagcctgca ggtcgtcaac ggtcatcacg ttgagttccc aaccgctcag ctgcgaagcc
    48901 agacgcacgt tctgaccgtt acggccaatc gcctgcgcca ggttaccggc ttcaacggcg
    48961 atatccatgg tgtgtttatc ttcatccacc acgatagaag caacgtctgc cggtgccatt
    49021 gcgttaatca caaactgcgc cgggttatca tcccacagga cgatatcgat acgctcgccg
    49081 cccagttcag tagaaaccgc ctgaacacgc gcgccacgca tacctacaca ggcacctacc
    49141 ggatcgatac gtttatcgtt ggttttcacc gcgattttcg cacgagaacc cggatcgcga
    49201 gccgctgctt taatttcaat cacttcttcg ccgatttctg gcacttcaat acggaacagt
    49261 tcgatcagca tttccggctt ggaacgagtg acgaacagtt gcgcgccacg cgcttccggg
    49321 cgaacggaat agagcacgcc acgaacgcgg tcgccagggc ggaagttttc acgcggcagc
    49381 atatcttcgc gcaggatcac ggcttcagca ttgttgccca gatccagaga gatgttgtcg
    49441 cggtttactt ttttcaccac gccggtgatg atttcacctt cgtgttcacg gaactgatca
    49501 accaccatcg cacgttcggc ttcacgcact ttctgcacga taacctgttt tgccgtctgg
    49561 gtagtgatac ggtcaaaggt aacagactca atctgatctt caacgtaatc gcccaggttc
    49621 aggctttcat cttcataacg tgcggcttca agggtgattt ccttggtcgg ctgggtgact
    49681 tcatcaacaa ctaaccagcg acggaaggtg tcaaaatcac cgcttttgcg atcgatctgt
    49741 acgcggacgt cgatctcttg ttcatatttt ttctttgttg ctgtcgccag cgcgctttcc
    49801 aatgcttcga aaatcttctc gcgaggtagc gccttttcat tggatacggc ttcaactaca
    49861 gccaaaattt ctttgttcat cgcgggcttt tcacctcatc cagactatta aaagtgggga
    49921 accaggttcg ccttctggat attactcagc gcgaacactt catctttacc ttcgacggta
    49981 actgtgatca tttcaccgtc taccgctttg ataacgccct gccatttacg acggttttgt
    50041 accgccatac ggagaaccag agtcacctct tctccgacaa aacgggcgta gtgttcagcc
    50101 gtgaacagtg ggcgatcgag acccggtgag gagacttcca ggttataagc aacggtgatg
    50161 ggatcttcaa catccagcac agcacttacc tggtggctca catcagcaca atcatcaaca
    50221 ttgatgccat cttcactatc aatatagatg cgcagtgtgg atgtgcgacc gcgaataaat
    50281 tcgatgccaa ccagttcaaa acccagggcc tcaactggcg cagtaatcat ctctgttaat
    50341 ttttgctcta atgtggacaa gcccaccccc aagacataaa aaaagggcct aaagcccagt
    50401 tattctgtag tcagataaca aaaaaccccg ataaatcggg gctttatata actgaaccct
    50461 ataaccgcaa ctgcggtctg gagcactttc cagaaggatt ttttcaaatc ccactacgaa
    50521 ggccgaagtc ttcacagtat atttgaaaaa ggactctaag ggaaagtggt tgcgggggcc
    50581 ggatttgaac cgacgacctt cgggttatga gcccgacgag ctaccaggct gctccacccc
    50641 gcgcctgaaa cgtggcaaat tctactcgtt ttgggtaaaa aatgcaaata ctgctgggat
    50701 ttggtgtacc gagacgggac gtaaaatctg caggcattat agtgatccat gccacatttt
    50761 gtcaacgttt attgctaatc acgtgaatga atatccagtt cactttcatt tgttgaatac
    50821 ttttgccttc ccctgctctc ccttaagcgc attattttac aaaaaacaca ctaaactctt
    50881 cctgtctccg ataaaagatg attaaatgaa aactcattta ttttgcataa aaattcagtg
    50941 agagcggaaa tccaggctca tcatcagtta attaagcagg gtgttatttt atgacgacga
    51001 ttctcaagca tctcccggta ggtcaacgta ttggtatcgc tttttccggc ggtctggaca
    51061 ccagtgccgc actgctgtgg atgcgacaaa agggagcggt tccttatgca tatactgcaa
    51121 acctgggcca gccagacgaa gaggattatg atgcgatccc tcgtcgtgcc atggaatacg
    51181 gcgcggagaa cgcacgtctg atcgactgcc gcaaacaact ggtggccgaa ggtattgccg
    51241 ctattcagtg tggcgcattt cataacacca ccggcggcct gacctatttc aacacgacgc
    51301 cgctgggccg cgccgtgact ggtaccatgc tggttgctgc gatgaaagaa gatggcgtga
    51361 atatctgggg tgacggtagc acctataaag gaaacgatat cgaacgtttc tatcgttatg
    51421 gtctgctgac caatgctgaa ctgcagattt acaaaccgtg gcttgatact gactttattg
    51481 atgaactggg cggccgtcat gagatgtctg aatttatgat tgcctgcggt ttcgactaca
    51541 aaatgtctgt cgaaaaagcc tactccacag actccaacat gcttggtgca acgcatgaag
    51601 cgaaggatct ggaatacctc aactccagcg tcaaaatcgt caacccaatt atgggcgtga
    51661 aattctggga tgagagcgtg aagatcccgg cagaagaagt cacagtacgc tttgagcaag
    51721 gtcatccggt agcgctgaac ggtaaaacct ttagcgatga cgtagaaatg atgctggaag
    51781 ctaaccgcat cggcggtcgt cacggcctgg gcatgagcga ccagattgaa aaccgtatca
    51841 tcgaagcgaa aagccgtggt atttacgaag ctccggggat ggcgctgctg cacattgcgt
    51901 atgaacgtct gttgaccggt attcacaatg aagacaccat tgagcagtat cacgcacatg
    51961 gtcgtcagtt gggccgtctg ctgtaccagg ggcgttggtt tgactcccag gcgctgatgc
    52021 tgcgtgactc tctgcaacgc tgggttgcca gccagatcac tggtgaagtt accctggagc
    52081 tgcgccgcgg gaacgattat tcaatcctga ataccgtctc agagaacctg acctacaagc
    52141 cagaacgtct gacgatggaa aaaggcgact cggtgttctc gccagatgat cgtattggtc
    52201 aattgaccat gcgcaacctg gatatcaccg atacccgcga gaaacttttc ggttatgcca
    52261 aaactggcct gctctcctcc tctgccactt caggcgtgcc gcaggtggag aatctggaaa
    52321 acaaaggcca gtaattcgac atcaaccctg cccttcaggg gtggggttac cttgcttatc
    52381 ctctgtaaac tcctttctga gcatcacata atcgaaaaca tcatggtttg catccacaac
    52441 ccgtgagtct cctttatagt gtgcaatcac ttcctcaagc gtttgcggct gttccggctt
    52501 tgttaccccc atccacgcat taataaggta attattgtaa gctgtcgaaa agatgttgtt
    52561 ttccgttgtg cgatccacgc catccccaag aacggggcta taccagataa acatcgggac
    52621 atgatatgcc tgctggctag cctccctgcc tccatgaaaa tagacgttct tcttcgtagg
    52681 gtcacgttcc aggccgtggt cggcaaaata catgaccgag gcgcggcgat cttttaataa
    52741 ttcaaaaacc tgacctagca aactatctgt gtaatgaatg gagttgtcat agcaggcatc
    52801 ctgatcgtcc tgcggttgaa acacggcgct ggattgcgga taggcgctac aagccggttc
    52861 atggcttcca tttaaatgaa gaacaatcag tttcttttgc tgcgtatttt gctgtaatgc
    52921 ttgcgataaa tgcggcaaca acaattcatc aaatcctctg acataaactg tttccatggc
    52981 gcgcatggcg atactggtaa ctgctgtacc attctgccga aaagcggatt gcgagcttag
    53041 ccagaaagtc tgaaatcctg cttgattagc catattaata atgttgtcgg ggtaattatg
    53101 aatgtcatga ctcaaaacag aatcagcagt taaagaaagg ggaaccgaca gcgcagtgta
    53161 aggtgcgccg cttattgctt gattaaacag tttgatctgt tttctttgtg cttcaacttg
    53221 cggtgtcgta gagcgtgtat atccatacaa agacatattg tcgacacgta cagactcccc
    53281 cacaatcaac acgtaggtat caatacctgt atccctgact gataattgaa aatacggcac
    53341 cgtgtttgca attgagagta atctttgatg ctctttcgct gctaaagcaa aatagttgag
    53401 attgaaaaat ggcgtatagg tagcaaatcg cgacgctagt atatatggac tgaacgcttt
    53461 tttatttttt gcatctttat aagcaaattg acaagcggaa aataaactgc ccgaaatgac
    53521 aatcagcaat aatattcctg tcactttttt tgtcggcaag gaaacatcat attttattat
    53581 tactgccaaa aaaagaaggg ataaaaaagc aaaggcacat agataaggga tatacatccc
    53641 cagcattttg acaacttcat ccggatcact ctggagcaca ctaatcgcaa atccatcatt
    53701 gaaagttgta ccaaaggtac accatgaata gaggctaata ctcatgtctg ccgcagtcag
    53761 taacacaaat ggtgcggcaa caagcaggcg aagcggaagg gatgatatac gttttacaac
    53821 caggatggtc aggacgaaga acagcaatcg ctgaaccata tgcgcgccgg aagaggcgac
    53881 taagttcgta ataccaaaaa gaataacaca aagatacaac aaccaatgag atttaaaact
    53941 tctagcaaat ttgttgaata ctgtcatcca gaaatccaag ttcacaacca atataaacaa
    54001 ttatcaacaa tgttcaattt tatatcagac atgtttttca actctgatcc atatcagacc
    54061 gaaacactgt tagttttttc tttttaaatg atgcggccat tgcgttctca gtaattaaat
    54121 aatattatca atgcgtctct aaacattacg cagaacgtag ccatcagtag ttatactgac
    54181 aggagcagga tggtttatcg agaagtaaac atatccgtat tcgtaccgga actggagttg
    54241 acacaataaa gtgccaatta tgtcagcaga agggaaaagt ttataactaa ggcgtaccgg
    54301 caccatcgtt tcaaggtacc agctacgagt aaagcaactg gacgagatac agatacctga
    54361 caaccattcc tcagaccagg accaaaacga aaaaagacgc ttttcagcgt ctcttttctg
    54421 gaatttggta ccgaggacgg gacttgaacc cgtaagccct attgggcact accacctcaa
    54481 ggtagcgtgt ctaccaattc caccacctcg gcacggatac tactttttag ttcgggatat
    54541 cgctggtcgg cttagccgga gcagctggtt gagtttgttc ggttttcacc ggtgcactca
    54601 gattttccca ttcgctacct ttattggttt tgttgctatt gatgttaccc agcaccagac
    54661 tgatgatgaa gaataacgtt gccagcagcg ccgtcatgcg ggtcatgaag ttaccagaac
    54721 cacttgaacc aaacagcgta gcggaagcgc ctgctccgaa ggaggctccc atatcagcgc
    54781 ctttaccttg ctgcagcatg atcagaccaa caaggccaat tgccacaata aggaaaacta
    54841 ctaaaagagc ttcgtacata atcaacctgt tccttgcgga gttgccgcgt accaatgctt
    54901 caaccaataa agcgggagtt tttaatcttt cccactgaag cgggtgtgaa tactaaccaa
    54961 agcgaatgac cttcgcaagg gcaattttat cgcattgtat caactgcgga aaaaaacagc
    55021 aaaaccgatg tgttcgctga gaaaaaaggc gacaatcgcc gcctttttag ccagttatct
    55081 aacgctttaa acggctttta ctgcatcggc gatgcggtgt gcaaattcag tcacctgcgc
    55141 ttcgtcttcg ccttccacca tcacgcgaat taacggttcg gtgccggatt tacgcagcaa
    55201 cacgcgtcca cggttgccca gcgcagcttc aacctctgcg gtcacggctt taactgactc
    55261 atgctcaagt ggatcgccgc tacctgcggt gtaacgtacg ttaaccagaa tctgcgggaa
    55321 cattttcatg ccgctgcaaa ggtcgtgcag gctcatatgg ttacgtgcca tcgccgccag
    55381 cacctgcaag ccagcaacga tgccgtcacc ggtagtagtt ttatccagca ggatcacatg
    55441 accggaattc tctgcaccga tacgccagcc tttctcctgc attttttcca gtacgtagcg
    55501 gtcacccact ttcgcgcgcg caaatggaat tcctaactgt ttcagcgcca gttcaagccc
    55561 catgttgctc atcaatgtac ccacagcgcc accacgcagc tggccctgac gaagaccttc
    55621 acgcgcgatg atatacatga tctgatcgcc atcgacttta ttgccttcat ggtcaaccat
    55681 aatcacgcga tcgccatcgc cgtcgaaggc aataccgaga tccgcttttt cagccagcac
    55741 acgagcctgg agcgcgcgaa cgtcggtagc ccccacttcg gcattgatgt ttacaccgtt
    55801 tggctcacaa ccgatagcga taacgttcgc ccccagttcg cgcagcacgt tcggcgcgat
    55861 gtgataagtc gcaccgtttg cacaatccac cacaatcttc agttcactga ggctaagttc
    55921 gttcgggaac gtggctttgc aaaactcgat atagcgaccc gcggcatcaa cgatacggct
    55981 ggctttaccc agttctgccg aatcaacgca gctgatctcc ttttccattt ccgcttcgat
    56041 ggcctcttct accgcatccg gcagtttggt gccgtcgata gagaagaatt taatgccatt
    56101 atcgtagaac gggttatgcg atgcagatat cacaattccg gcctctgcgc ggaaggtacg
    56161 cgtcagataa gccacggccg gtgttggcat cgggccagtg aagagtgcgg aaaggcccgc
    56221 tgccgccaga cccgcttcca gtgctgactc cagcatatag ccagaaatac gcgtgtcttt
    56281 accaataata atcttacggg agccgtggcg cgccagcact ttacccgcgg cccaacccag
    56341 cttaagcaca aaatcaggtg tgatcggcgc atcccctaca cgaccacgaa tcccatcggt
    56401 accgaaatat ttacgattac tcatagcgtt tgttttcctt tgcagacaga gtggcttcca
    56461 ccacccgcat cgcttctacg gtttctttga cgtcatgaac acgaatgatg tgcgcgcctt
    56521 gcattgcggc aatgaccgca caggccagac taccgctcag gcgctcggac ggccccacgt
    56581 tcagcagctg cccaatcatc gattttcgtg acatacccac caacagcggc aggttgaaat
    56641 ggtgaaattc agccaggcgc gccagtaatg aatagttatg ggagagattt ttaccgaaac
    56701 cgaatccggg gtcgagcaac aatttctctt ttgcgatacc cgcctgctcg caacgtgcta
    56761 tttgctcaat aaagtagcga ttcacttctg caaagacatc gtcatacttc ggagcttcct
    56821 gcatggtttt tggatttccc tgcatatgca tcagacaaac cggtaaaccg gtttctgcag
    56881 ccgcctccag agcgccaggt tcggaaaggg agcggatatc attaataatg tgagcgccaa
    56941 ctttcgctga ctcacggatg acttctggtt tggatgtatc gaccgagatc cagacttcga
    57001 agcgttgagc aattgcctca accacaggaa taacacgttg caactcttct tcaacgctaa
    57061 cttccgccgc ccctgggcgc gtggactcgc caccaacgtc aatgatcgtc gcgccagcat
    57121 tgatcatcag attcgcatgt ttcaccgcat ctatcagcga gttatgcgtg ccaccatccg
    57181 aaaaggaatc aggcgtgacg ttgaggatcc ccattacgtg aggatggcta aggtccagtg
    57241 aagtaccctg ggcaaagagt ttcatgatgt tatccctggt atgaattgat aagaaaaaac
    57301 cccggagcac gccccggggt tttcggtaca aatacagtca tctgatgcgg gaacttactt
    57361 gtcgcctaac tgctctgaca tggtgttacc cgggttcggc gtacgcggtt catcaaccgg
    57421 acgaggagcc tttggactac cattgtcgcc agaattgtta gaagcgcctg gttcttccca
    57481 gcccgctggc ggacgtacat cgcgacgtgc catcaggtca tcaatctgcg gtgcgtcgat
    57541 agtctcatat ttcatgagag catctttcat cgcatgcaga atatccatat tgtcggtcag
    57601 aagctgacgc gcacgattat agttacgctc aatcagtgct ttcacttcct ggtcgatgat
    57661 acgtgcagtt tcatcggaca tatgtttcgc tttcgctacg ctacggccga ggaatacttc
    57721 accttcttct tccgcgtaca gcagtggacc caatttctca gagaagcccc actgggtcac
    57781 catgttacgt gccaggttgg tcgcaacttt aatatcgttg gacgcaccgg tagaaacatg
    57841 ttccggcccg tagatgatct cttctgccag acgaccaccg tacagcgtag aaatctggct
    57901 ttccagtttc tgacggctgg cgctgattgc gtcgccctca ggcaagaaga aagtcacacc
    57961 cagcgcacga ccgcgtggga taatcgtcac tttgtgcacc ggatcgtgtt ccggcaccag
    58021 gcgaccgata atcgcatgac ccgcttcgtg gtaagccgtt gattctttct gcgcttccgt
    58081 catcaccatg gagcgacgtt ccgcacccat catgattttg tctttcgctt tctcgaactc
    58141 aaccatcgac acaacgcgtt tgttgccacg agcagcgaac agtgccgctt cgttcaccag
    58201 gttcgccagg tcagcaccgg agaaaccagg agtaccacgg gcaatgattg ccgcgtcgat
    58261 atcgggggcc aatggtacgc gacgcatgtg aactttcagg atctgctcac gaccgcgaac
    58321 atctggcaag ccgaccacaa cctgacggtc gaaacggcca ggacgcagca gggccgggtc
    58381 gagaacgtcc ggacggttag tcgcggcgat aacgatgata ccttcgttac cttcgaagcc
    58441 atccatctca accagcatct ggttcagagt ctgttcacgt tcatcgtgac caccgcccag
    58501 accagcgcca cgctggcggc ctacggcgtc gatttcatcg ataaagatga tgcacggtgc
    58561 cgctttcttc gcctgttcga acatgtcacg aacacgggat gcacccacac cgacgaacat
    58621 ttctacgaag tcagaaccgg agatagtaaa gaacggaact ttcgcttcgc ctgcaatcgc
    58681 tttcgccagc aacgttttac cggtacccgg aggaccgacc atcaggacgc ctttcgggat
    58741 cttaccgccg agtttctgga agcggctcgg ctcgcgcaga tactctacca gttcagcaac
    58801 ttcttctttt gcttcgtcgc agcccgcaac gtcagcaaag gtcgttttga tctgatcttc
    58861 cgtcagcatg cgcgctttgc tcttaccaaa cgacatggca cctttgccac cgccgccctg
    58921 catttgacgc atgaagaaga tccagacacc aatcagcaac agcatcggga accaggagat
    58981 gaagatagaa gccagcaggc ttggttcttc aggcggttca ccgacaacct tgacgttctt
    59041 ggtcaacagg ttatccagta atttcggatc ctgaaccgga atgtaagtgg tataacggtt
    59101 actatctttc ttggtaacgt tgatttcacg tccgttgata cgcgcttcac gaacctggtc
    59161 gttattgacc tcttgtagga aggtagagta atccacctta cggccattag actcgctggg
    59221 cccaaagctc tggaatactg acatcagcac aacggcaatg accagccaga gtattaggtt
    59281 tttcgccatg tcactcaagg gattaacctc ttattacaac tgtgttaaaa acagcgtcag
    59341 gatactctat atccagcatc tttcaaactt tcgtctgaaa tctcccggtt agggtttacg
    59401 cccggtcgct acaatataca cttcccgcga acgtgcacga gaagagtccg gcttacgaac
    59461 tttgaccttc gtaaacaggg agcgaatttc ccttagatac tcatcgaaac cttcgccctg
    59521 gaacaccttc actacaaaac tgccacctgg cgctaataca tcacgacaca tttctagcgc
    59581 cagttccacc agatacatgg cacgggggat atccaccgcc ggtgttccgc tcatgtttgg
    59641 tgccatatcg gacatgacaa cctggacttt gctgtcgcca acgcgctcca gcagtgcttt
    59701 catcaccagt tcatcacgaa aatcgccctg aagaaagtcc acaccaacga taggatccat
    59761 aggtaaaaga tcgcaagcga tgatgcggcc tttgccgcca atttgggtga ccacatattg
    59821 tgaccaacca cccggagcag caccaaggtc gacaaccgtc attcccggtt taaagagttt
    59881 gtcactttgc tgtatttcat caagtttaaa ccaggcacgg gaacgtaacc cctttttctg
    59941 tgcctgttga acatatttat cgctaaagtg ttcctgaagc cagcggctgg agctggcaga
    60001 acgcttctta cctgtcattt aactttccca tggggataac tcatcgtaac caattgcgta
    60061 aatttttacg cgcctatttg gtgatatatg ggagatggcg gtagaatgac ccgttttcaa
    60121 tcccaacgta agcaaaaaat acgatgaatc tgagtactaa acaaaaacag cacctgaaag
    60181 gtctggcaca tccgctcaag ccagttgttc tgcttggcag taatggtttg accgaagggg
    60241 tgctggccga gattgaacaa gcgttagagc accatgaact catcaaggtg aaaatcgcca
    60301 ccgaagatcg cgaaactaaa accttgatcg tggaagctat cgtgcgcgaa accggcgcct
    60361 gtaatgtaca ggtcatcggt aaaacgctgg tgctttatcg cccaactaaa gaacgtaaaa
    60421 tctcgctgcc acgctaagat tatcctaaag ttacacacat ttgctgtgta aaacgagggg
    60481 ttttccgcag gcaggagagc aaaatgccac gctctgttcg ttgataaaag gccgcatagc
    60541 ggcctttttc ctttctttac aatacatcaa catcttgagt attgggtaat tcttacaggt
    60601 attccacctt aattacttca aattctactt cgccgcccgg cgttttgatg accacaacat
    60661 catcttcttc tttgccgatc aggccacgag caataggtga gtttacagaa atcaggtttt
    60721 gtttaaagtc agcttcgtca tcgccaacga tgcgataagt ctgttcttcg tcagaatcca
    60781 gattcagcac cgttacggta gcaccaaaaa taacgcgccc attgttgggc attttggtga
    60841 catcaatcac ctgcgcgttc gacagcttgg cttcgatgtc tttgatacgg ccttcgcaga
    60901 agccctgctg ttcacgagct gcgtggtatt cggcgttttc tttcaggtcg ccatgctcac
    60961 gcgcttccgc gatagcagca atgatttcag gacggcgcac agatttcaga aaatccagct
    61021 cttcgcgtaa tttttcagcg ccgcgtaagg tcatcggaat agcttgcatt tgttatacct
    61081 cttgaatatt cctgatgggg caagtcttca cccaccccgg ctgttcggcc tgcccggcat
    61141 aactcctcac cgggaccaga agcaaaaaaa taccgacccg ggtacaagtc ccaggtcagc
    61201 tacaattcac attttgatag tcattttacc ctgaagttcc cgaagggtca tcgtttactt
    61261 tatagggcgt tgcgccgtag tatgacggct cgattccagg ttgttagcgc gagattatgc
    61321 gattttccag atttatcatc ggattgacca gctgtatagc gttcagtgtt caggccgcga
    61381 atgttgatga gtacgttact caactccccg ctggtgccaa ccttgccctg atggtgcaaa
    61441 aagtcggcgc gtcggccccc gctattgatt accacagtca gcagatggcg ctgcccgcca
    61501 gtacgcagaa agtgattact gcgctggcgg cgttgattca actcggcccc gattttcgtt
    61561 ttaccacgac gcttgaaacc aaaggcaatg tggaaaacgg cgtacttaag ggtgacttag
    61621 tggcgcgatt tggtgccgat ccgacgttaa aacgtcagga tattcgcaat atggtcgcga
    61681 ctttgaaaaa atctggcgtc aaccaaatcg atggcaatgt gttgatagat acctccattt
    61741 tcgccagcca cgataaagcc cccggctggc catggaatga catgacacaa tgctttagcg
    61801 ctccgcctgc cgccgccata gttgaccgca actgtttctc cgtctcgctc tacagtgccc
    61861 aaaaacccgg tgatatggcc tttatacgcg tggcatctta ttaccccgtt acgatgttca
    61921 gccaggtacg caccctcccc cgtggttctg ccgaagcgca atactgcgaa ctggatgtgg
    61981 tgccaggcga cctgaaccgc tttacgctga cgggatgcct gccacaacgt tctgagccgc
    62041 tcccgttggc ttttgccgta caggatggag ccagctatgc cggggcaatt ctgaaagatg
    62101 agttaaaaca ggcgggtatc acctggagtg gaacactgct gcgccagact caggttaacg
    62161 aacctggaac ggtagttgcc agtaaacagt cggccccgct gcacgatctg cttaagatta
    62221 tgctgaaaaa gtcagacaac atgatcgccg atacggtttt ccgcatgata ggccatgcgc
    62281 gcttcaatgt gcctggaaca tggcgggccg ggtcggacgc cgtgcgtcag atcctgcgcc
    62341 agcaagccgg tgtcgatatt ggaaacacca ttattgccga tggttcaggg ctttcgcggc
    62401 ataacctgat tgcccccgcc accatgatgc aggtgctgca atacattgcc caacacgaca
    62461 atgaacttaa ctttatctcc atgctgccgc tggcgggcta tgacggctct ttgcagtacc
    62521 gtgcaggtct gcatcaggcg ggcgtggatg gcaaagtctc agcgaaaacc ggttcgttgc
    62581 agggggtata taacctggcg ggattcatta ccacagcgag cgggcaacga atggcgtttg
    62641 tgcaatatct ttctggctat gcagtagaac cggcggatca gcgtaatcgc cgtattccgt
    62701 tagtgcgttt cgaaagccgt ttgtataaag atatttacca gaacaattag tcaaaaagaa
    62761 accccggcat atggccgggg tttcagatta ttgacaaagt gcgctttgtt tatgccggat
    62821 gcggcgtgaa cgccttatcc ggcctacaaa atcgtgcaaa ttcaatatat tgcaatcatc
    62881 ttgtaggcct gataagtgta gcgcatcagg cttatttggc attggtcatt agtgattaac
    62941 gcttgtaaat gaactcaacg ccttcttcgt cgtcttcgtc ccagtcgtca tcccagtcct
    63001 catcatcctc ttcagcaatc tcttcaagct gctggcgatg ataatcatcc cacatgaatt
    63061 cgactttctc tggctgtttc gcttcttcag cctgcacgac cgggttttca atgataaagg
    63121 tcatcacatc ccagcagaga tctttcacgc ccagtccgct cgccgcagag atcagataat
    63181 atttatcttc ccagcccagc gcctcagcga tcgctttcgc tttctcttcg gcttctgcct
    63241 tatccagcag atcgatcttg ttgaacacta accaacgcgg tttcgccgcc agatcctggc
    63301 tgtatttttc cagctcgctg ataataatac gcgcgttttc aaccggatcg gtgccgtcaa
    63361 tcggatcgat atcgatgagg tgcaacagga cgcggcaacg ttccaggtgc ttcaggaagc
    63421 ggatacccag acctgcgcct tccgcagcgc cttcaatcag tcctggaata tcggcaacaa
    63481 cgaagctctt ttcgttgtcc attcgtacca cacccagact tggcaccaga gtggtaaacg
    63541 gataatccgc cactttcggt ttagccgccg ataccgcgcg aataaaggtc gatttccccg
    63601 cgtttggcat ccccaacata ccgacgtcag ccagcagcat cagctccagc agcagctcac
    63661 gcttatcacc cggcgtaccg ttggttttct gccgtggtgt acggttaacg gacgatttga
    63721 aacgggtatt gcccagaccg tgccagccgc ccttagcaac cagcagacgc tgaccgtgtt
    63781 tagtcatatc gcccatggtt tcaccagtac cctggtcgat tacacgcgta cctaccggca
    63841 ctttaatcgt gacgtcttta ccgcgcttac cggtacagtc gcggcttgcg ccgttttgac
    63901 cgcgctctgc acggaaagat ttttcaaaac gataatcgat aagcgtgttc aggttctcgt
    63961 cggcttccat ccatacgtca ccaccatcac cgccgtcgcc gccatccggg ccgcctttcg
    64021 gaatatactt ttcgcggcgg aagctcacgc aaccattacc gccatcacct gcaacgacca
    64081 gaatcgatgc ttcatcaaca aacttcattt tattctccgt aaatcattcg cctgcgcggg
    64141 gttgcgatac caccgttgta tgcttacgta atccgcccca aatacgatga ccaatggcgg
    64201 aatacatcgc gcccgcaacc acgacaaacg caccgagata acctaaaagg tttaacatcg
    64261 gtctggcgaa gaaatcgggc caggccagtg ataaaagatc tgaaaaaaac agcgtaaaca
    64321 gtggggtgag cgtgatgatc gcgctcacct gcgctgcctg ccagcgagcc atcgcttccg
    64381 ccagggcgcc atatcctacc aatgtattca gtccgcaaaa aattaaacat gcgagctgcc
    64441 agtggctaag ctgcgctatc actccaggct ttgccagagg gaagagcgcg attgtacata
    64501 aagtgtacag taaaaacaga atctgcggtg aggccagccg acgcaataaa accttttgcg
    64561 ccacgccata actcacccaa accgtcgccg caccgacccc aaagataact ccccaggtgt
    64621 aatcagtgag ctttgtaaat atctcgacca gactggtgtt aaaaaacatc accaggccgc
    64681 tcaggagcat caatgcccct acaacctgag tgctgcgcat tttctctttc aggataaata
    64741 cgctggcaac catcatgcca actggcgaga gttgcccaat cacctgcgaa gcggtcggac
    64801 tcaggtattg caaggatgag ctgaacagga tgaagttccc aaacagcccg gcggtcgcca
    64861 ctgccaacaa aatcaaccag cgtggcttac gaaacacgcg taatggcggc aacctcttct
    64921 tcaccgcaag aatggcacca aggccaatac tcgccatcaa gaaacggtaa aacacgattg
    64981 tcggaggttc catcacctcc agcacctgct tcattgcgat tggcaacgcc ccccagcaaa
    65041 ttgctgtggt gagcgccaaa agaatgccaa tgcctgcctg ctgcttcata ccgtattccc
    65101 tgcaaagcgc atttgcggat taacgtcgca ccatgctcat ttttaccggt tatcgaatgt
    65161 aaaaagcccc gcaacgtgtt gcggggcttt catccgttac cgggacgcga aaaacttatt
    65221 cagcttcgat gctgataaat ttacggtttt tcgggccttt aacttcgaat ttcactttac
    65281 cgtctgcttt agcaaacaga gtgtggtcac gaccgcaacc tacgttagcg ccagcgtgga
    65341 atttggtacc acgttgacga acgatgatgc tacccgccag aacggattcg ccaccgaaac
    65401 gcttaacgcc caggcgttta gcttctgaat cgcgaccgtt acgtgtggag ccgccagcct
    65461 ttttatgtgc catttgaaat ctctcctcag gtcttaggcg ctgatgccag taattttcac
    65521 atcagtgaac cactgacgat ggccctgctg cttacgatag tgtttacgac gacgaaactt
    65581 aacgatttta actttctcgc cacgaccgtg agcaacaact tcagctttga ttacgccgcc
    65641 atcaacgaaa ggaacgccga ttttgacttc ttcaccgttt gcgatcatca gcacttcagc
    65701 gaactcaaca gtttcgccag ttgcgatgtc cagcttttcc aggcgaacgg tctgaccttc
    65761 gcttactcgg tgttgtttac caccactttg gaaaaccgcg tacatataaa actccgcttc
    65821 cgcgcacacc ttttcaatga ttcagagtgc gctataaata ttcacaatag ggcgcgaata
    65881 ttacgcaaaa cgcacgcctt tgacaagtgc tacagtcaat acacgaagaa aaaaaacaca
    65941 acttgtacgg taacgtttat ctgtgccatt ttttcagtac aatcacccta tattcctaac
    66001 cataaaccct aagttgcctt tgttcacagt aaggtaatcg gggcgaaaag cccggctttt
    66061 gcgatgaatt tagaaaaaat caatgagtta accgcgcaag atatggcggg tgttaatgcg
    66121 gcaatccttg agcagcttaa ttccgacgtc caactgatca atcagttagg ctattacatc
    66181 gtcagcggcg gcggtaaacg tattcgtccg atgattgctg tactggctgc acgagctgtt
    66241 ggctatgagg gaaatgcgca tgtcaccatt gctgccttga tcgagtttat ccacacggcg
    66301 actctgctac acgacgacgt tgtggatgaa tcagatatgc gcaggggtaa agctaccgcc
    66361 aacgctgcat ttggcaatgc cgccagcgtg ctggtaggcg attttattta tacccgcgcg
    66421 ttccagatga tgaccagcct cggttcgctc aaagtgctgg aagtcatgtc agaagccgta
    66481 aacgtcatcg cagaaggtga agttctgcaa ctgatgaacg ttaacgatcc ggacatcact
    66541 gaagaaaact acatgcgcgt tatctatagc aaaaccgcgc gtctgtttga ggctgccgcg
    66601 cagtgttccg ggattctggc tggctgtacg ccggaggagg agaaaggcct gcaggattat
    66661 gggcgctatc tcggcactgc tttccagttg atcgacgatt tactcgatta caatgccgat
    66721 ggcgaacagt taggtaaaaa cgtcggcgac gatctgaacg aaggtaaacc gacgctgccg
    66781 ctgctgcatg cgatgcatca tggcacacca gaacaggcac agatgatccg taccgccatc
    66841 gaacagggta acggtcgcca tcttctggaa ccggttctgg aagcaatgaa cgcttgtgga
    66901 tctcttgaat ggacgcgtca gcgcgccgag gaagaagcag acaaagccat cgcagcgtta
    66961 caggtgctcc cggacacccc ttggcgagaa gcactcatcg gcctcgcgca catcgctgtt
    67021 caacgcgatc gttaatcccc tcccctcatc ccgcgcagcg cgcggggtgg ttccagtaaa
    67081 ttccataaaa acacttactc agctctataa cacctgaaaa acaggctgtg catctttata
    67141 tattctgaat attcacactc cttacatgaa ctttttagag caatcggcca tcaggagtat
    67201 agtgatgctc gacagaagaa gtgttctgaa tgaaagcgac taacttaagg agtgaggaaa
    67261 gatggaaagt aatttcattg actggcatcc cgctgacatc attgcgggtt tgcgcaagaa
    67321 gggaacgtca atggcggcgg agtctcgcag aaatggtttg agttcctcaa cgctggcgaa
    67381 tgcattatcg cgcccatggc cgaaaggaga gatgattatt gcgaaagccc tgggaactga
    67441 cccctgggtt atctggccat cacgctacca tgatccgcag acccatgagt ttatcgacag
    67501 aacgcagttg atgcgtagct acactaaacc gaaaaaatga gtggtctggc ggtagccccg
    67561 cgaacggggc tgccagctct cagacgatta ttcgcctttc acacgctcaa tatttgcacc
    67621 taaagcgcgc agtttgtctt caatgcgttc gtagccacga tcgatgtgat aaatacgatc
    67681 aaccaccgtc gtcccttccg caatacagcc agccagcacc aggcttgctg atgcacgcag
    67741 atcggttgcc ataacctgtg cgccagaaag tttttcaaca ccgtgacaaa taacggtatt
    67801 gctttcgatt tcggcatgcg cgcccatacg gctaagctcc ggcacatgca taaagcggtt
    67861 ttcaaagacc gtttcggtga tgaatccggt cccttctgcc accaggttca acagcgtgaa
    67921 ctgggcctgc atatcggtcg ggaatgccgg atgcggcgcg gtacgtacgt taacagcctt
    67981 cggacgtttg ccatgcatat ccaggctaat ccagtcttcg ccgacttcga tgtccgctcc
    68041 agcatcacgc agtttcgcca gcacggcgtc cagagtatct ggctgcgcgt tacggcagat
    68101 aattttgccg cgagaaatcg ccgccgccac caggaaagta ccggtttcga tacgatccgg
    68161 tagcacgcga tagacaccgc cgcctaaacg ttccacacct tcgatgacga tacgatcggt
    68221 gccctgaccg ctaattttcg cacccagcgt aatcaggaag ttcgcggtat cgacgatttc
    68281 cggttcacgc gctgcgtttt caataatcgt ggtaccttcc gccagggttg cagcacacat
    68341 gatggtcacc gttgcgccaa cgctgacttt atccatcacg atatgtgcgc ctttcagacg
    68401 accatcgacg gaagctttaa cgtaaccttc ttccagtttg atggtcgcgc ctaattgttc
    68461 gaggccagaa atgtgtagat caaccggacg cgcaccgatc gtacaaccgc caggcagtga
    68521 aacttgcccc tgaccaaagc gcgctaccag cggccccagt gcccagatag aagcacgcat
    68581 ggttttaacc agatcgtaag gtgcgcagaa tacattaacg tcgcgggcat caatatgcac
    68641 agaaccatta cgttctactt tcgcacccag ctggcttagc agcttcattg atgtatcgac
    68701 gtctttcagt ttcgggacgt tctggatctc taccggttct tccgccagta gagcggcaaa
    68761 aaggataggc agagcagcat ttttagcgcc ggaaattgtg acttcgccct ggagcttcgt
    68821 tggcccctga acacgaaatt tatccattta gtttgttctc agttaacaat tcatatccgc
    68881 taccggcgaa tcgcccatag ctcaaaagcc gttcagtttg cgatcgcgcg cccactccgc
    68941 aggggtatac gctttgatcg acacagcatg aatgcggtta tccgcaatat attccatcag
    69001 cgggccatag accgtctgct gttttttaac ccgactcatg ccgtcaaaca actcacccac
    69061 ggcaataacc tgaaagtggc tgccatcgcc ggaaacgtgg acttcctgga gggagagagc
    69121 gttcatcaac acgctctgaa tttcattatt ttccatggga tcttcaatca tcagttaata
    69181 aaccagcgaa acatcttaga gcaaagttgc gctggcataa ataagcaaaa agcctcgctg
    69241 ataaatcaga caaggctcga cttgcaggca ggtttgccgg acaggcggtt aacgccatat
    69301 ccggcctgaa aaaatttaac gaggcagaac atcagcaggc aaattataca atttcgccag
    69361 ggtatacact ttgtcgttta ccccctgaag cgtcacattg ttgccctgct ttttcgccag
    69421 atcgataaga tggagcagca gtgccagtcc ccccgtatcc acgcgggaga cacggctaag
    69481 atcgatgcag gtaatcccct tcaccgcttc ctcacgcatt tcccaaagcg gtagcaaaac
    69541 gtcctgatcc agctctccgg ataacgccag cgtgtcaccc gtctgcatcc agctcagtga
    69601 ctcgctcatt attttttctc ttccagagtg attttctgtt gagaaatcga tttcagttgc
    69661 gctgtcaggc cgtcgatacc tttggtacgc agcagcgttc cccactcgtt ttgtttggtg
    69721 gtgatcatac tgacgccttc agcaatcatg tcgtaagcct gccagttgcc cgtctgggag
    69781 tttttacgcc actggaagtc cagacgcacc ggcggacggc cattcgggtc aataatggta
    69841 acgcgaatag gcacaatggt tttatcgccc agcggctgtt ctggcgcaat ctgataggtt
    69901 tgaccgtgat acatcgccag cgcctgaccg taagcctgct tcaggtactc acggaaagcg
    69961 gcaaagtagg cttcacgttg agcaggggtc gcactcttgt aatactggcc cagcaccagc
    70021 gcaccggcgt atttcacctg tacgtatggc agcagttcct gatcaacaat ggtacgcaga
    70081 taatccgggt tggcccgaat ttgcggttgc tcattcttca ggcgatcgaa cgttttctgc
    70141 gccgcctcgt ccatcagctt atacggattg gtctggtctg ccgcggttgc cgcactcaga
    70201 ggtgcaatca ccagcaaagc gaccatcatt aaacgtttaa acatgcgtcg gttctcctga
    70261 aattatttcg ttgtacccac aggttcagtg gtttcattat tacctggcgc agcagctggc
    70321 gcatcgccac tattcttatt gtcatcgcct ttactaccgt aaaggaactg accaatgaga
    70381 tcttccagca ccatcgcaga cttagtgtcc tgaattgtat cgccatcctt caggatagca
    70441 gtccccagtt ccgggtcttc aaaaccgacg tttaatgcca gatattgttc ccccagcagg
    70501 ccggaagtac gaatgctcag cgaactggta tctggaatgt ggttataacg ttgttcaatt
    70561 tccagcgtta cgcgcggcag ataggttttc gggtccagcg taatatccgc cacccgaccc
    70621 acaacaacgc caccaatact gaccggagag cgcgctttca ggccgccaat gttatcgaac
    70681 gtcgcataaa gcgtgtaggt cggttcagta cgtatggacg tcacgttcgc cgccttcagg
    70741 caaacaaaca gcgccgccag cagtgctgct aataaaaaga tacccaccca aatttcattt
    70801 tttttcgttt gcatgaactc aattcccaaa catcaatgcg gtcagcacaa aatccagccc
    70861 cagaacagcc agagacgagt ggacaacggt gcgagtggtt gcccggctaa tcccggcaga
    70921 cgtcgggatg gcgtcgtagc cgttaaacaa cgaaatccac gtcaccgtga tggcgaacac
    70981 cacgctctta atcagacagt tgaccagatc catacgccag tcgacggcat tttgcattgc
    71041 cgaccagaag aacccgctat caatgccttt ccaactgacg ccgaccagag atccgcccca
    71101 gatccccacg gcgacgaaaa taaccgtcaa cagtggtaat gaaataaccc cagcccagaa
    71161 acggggagaa ataacccgac gcagcggatc caccgccatc atctccatac tggagagttg
    71221 ctctgtagcg cgcatcaggc cgatttctgc ggttagcgcc gaaccagcac gcccggcaaa
    71281 caacaacgcg gcaaccaccg gccccagttc acgcagtagc gataacgcca ccagcatacc
    71341 cagactggtt tccgcactat aagtggtcag aaccagataa ccttgcagcc ccaacaccat
    71401 tccgatgaac acgccagaaa ccacaataat cagcatcgac aggacgccga cattatagag
    71461 ctggcgcacc agcagcggcg catgtttgcg aaattccggt ttgccgacca gcgcattgaa
    71521 taacattaac ccggcccgcc cgaacgttct cagggtttta atccctttat gtccgagcga
    71581 cgccagcgca tttaacagca tgagtggctt aactccctgg taaaagatca gcgtgataat
    71641 cgccggcagg atagcggaac ggaacaggcc cgtcagctat cccgtccaga aactgacgta
    71701 cgcgcggatc aggattcgcc tgcaacgcct gggcactgcc atgagcgaca atttttttgt
    71761 ccgccaggat ccaggcgtga tccgcaatac ttaacacttc cggcacatcg tgagaaacca
    71821 ccacacaagt cacgcccagc gcgctgttca gctcagaaat cagcttcacc agtacgccca
    71881 tggtgatggg atcttgccca acaaaaggtt catcaaacat gatgagatcc ggctccagcg
    71941 caatcgcacg cgccagcgct gcacgccgcg ccatcccacc ggaaagttca gaaggcatta
    72001 gtttagccgc tccacgcagc cccacggcct ccagcttcat catcaccgta ctatgcaaca
    72061 atggcgcggg aagttgggta tgttcgcgca gtggataggc gacgttgtca aatacgttca
    72121 tatcagtgaa caacgccccg gactgaaata acatgctcat ccgtttgcgc actgtataca
    72181 ggcgcgaacg agacatcgcc ggaatattct caccatcgaa aaggatctca ccatgatctg
    72241 gtgcgatttg cccgccaatc agacggagta gcgtcgtttt accgatgccc gatggcccca
    72301 tgatcgccgt gatcttccct cgcggcacgg tcagggaaat attatcgaag atgcagcgat
    72361 tgccacgcgt aaaactgaca tcgcgcatat cgactaaatt cgccacagac tgctccataa
    72421 ttcacccttc gtcttgcgtt gattttctaa gcatggcgct caatttaacc ttgaacccaa
    72481 catatttaca gaatattacc cgccgtggtt agcgaaagct ggcatttgtt ttacttttta
    72541 gccgcataaa gtcaaaatta agaatccgtt acggctttct gaaaatcttc agcggaccgg
    72601 cgagtatacc tgaagaaagg acgttagatg cttttagcta cggcactgtt aattgttggt
    72661 ttacttttgg tcgtttacag tgccgaccgc ctggtttttg ccgcgtctat tctttgccga
    72721 acctttggca tcccgccgct gatcatcggc atgacggtgg tcagtattgg tacatcgtta
    72781 ccagaaatca tcgtctcgct tgccgcgtct ctgcacgaac aacgcgattt agccgttggt
    72841 acagccctcg gctcaaacat tatcaatata ttgctgatcc tcggtctggc tgcgctggtt
    72901 cgtcctttta ccgtccattc tgatgttcta cgccgtgaat tacccttaat gttgttggtc
    72961 agcgtggtgg ccggttccgt actctatgac ggacaactta gtcgaagcga tggtatcttt
    73021 ctcctctttc tggctgtgct atggctgctg ttcattgtta aacttgcacg tcaggctgaa
    73081 cgtcagggga ctgacagcct gaccagagag cagcttgcag agctgccgcg tgacggcgga
    73141 ttgcccgtcg cgtttttatg gctcggcatt gcgcttatca tcatgccagt ggccacgcgg
    73201 atggtggttg ataacgccac ggtgctggcg aattactttg ccatcagcga gttgacgatg
    73261 ggtctgacgg caattgctat cggaaccagc ctgccggaac tggcaaccgc aatagcgggg
    73321 gttcgcaaag gtgaaaacga cattgctgtc ggaaatatca ttggcgcaaa catttttaat
    73381 attgtcatcg tgttgggttt acccgcgctg ataacgccag gagagattga tccactggcg
    73441 tacagtcgtg actacagcgt gatgttgctg gtgagcatta tttttgcgtt gctgtgctgg
    73501 cggcgctccc cgcaaccggg ccgtggtgta ggggtattat taactggcgg atttatcgta
    73561 tggctggcga tgttgtactg gttatcgcca atactcgttg aataactgga aacgcattat
    73621 gtcgcacgta gagttacaac cgggttttga ctttcagcaa gcaggtaaag aagtcctggc
    73681 gattgaacgt gaatgcctgg cggagcttga tcaatacatc aatcagaatt tcacgcttgc
    73741 ttgtgaaaag atgttctggt gtaaagggaa agttgtcgtc atggggatgg ggaaatcggg
    73801 gcacatcggg cgcaaaatgg cggcaacgtt tgccagcacc ggtacacctt catttttcgt
    73861 ccatcctggt gaagccgcgc atggtgattt aggcatggtt accccacagg atgtggtgat
    73921 tgctatctct aactcaggtg aatccagcga aatcacggcc ttaattccag tgcttaagcg
    73981 tcttcacgta ccgttaatct gcatcaccgg tcgcccggag agcagcatgg cgcgcgccgc
    74041 agatgtgcat ctgtgtgtta aagtagcgaa agaagcctgt ccgttagggc tggcaccgac
    74101 cagcagcacc accgccacgc tggttatggg cgatgccctc gctgtcgcgc tgttaaaagc
    74161 acgcggcttt actgctgaag attttgcgct ctcacaccca ggcggcgcac tgggtcgtaa
    74221 acttctgctg cgcgtaaacg atattatgca tacgggcgat gagatcccgc
//
LOCUS       DAMZET010000007        68002 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.007, whole genome shotgun sequence.
ACCESSION   DAMZET010000007 DAMZET010000000
VERSION     DAMZET010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 68002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.007"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(31..987)
                     /gene="ytfQ"
                     /locus_tag="POB34_000552"
     CDS             complement(31..987)
                     /gene="ytfQ"
                     /locus_tag="POB34_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HDI5917554.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            1297..1827
                     /gene="ppa"
                     /locus_tag="POB34_000553"
     CDS             1297..1827
                     /gene="ppa"
                     /locus_tag="POB34_000553"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HDI5917555.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(1907..2257)
                     /gene="chpB"
                     /locus_tag="POB34_000554"
     CDS             complement(1907..2257)
                     /gene="chpB"
                     /locus_tag="POB34_000554"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HDI5917556.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(2251..2502)
                     /gene="chpS"
                     /locus_tag="POB34_000555"
     CDS             complement(2251..2502)
                     /gene="chpS"
                     /locus_tag="POB34_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HDI5917557.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            complement(2715..3056)
                     /locus_tag="POB34_000556"
     CDS             complement(2715..3056)
                     /locus_tag="POB34_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HDI5917558.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(3059..6838)
                     /gene="tamB"
                     /locus_tag="POB34_000557"
     CDS             complement(3059..6838)
                     /gene="tamB"
                     /locus_tag="POB34_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HDI5917559.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(6835..8568)
                     /gene="tamA"
                     /locus_tag="POB34_000558"
     CDS             complement(6835..8568)
                     /gene="tamA"
                     /locus_tag="POB34_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HDI5917560.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            8774..9412
                     /gene="msrA"
                     /locus_tag="POB34_000559"
     CDS             8774..9412
                     /gene="msrA"
                     /locus_tag="POB34_000559"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDI5917561.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            9735..11078
                     /locus_tag="POB34_000560"
     CDS             9735..11078
                     /locus_tag="POB34_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDI5917562.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(11140..11346)
                     /locus_tag="POB34_000561"
     CDS             complement(11140..11346)
                     /locus_tag="POB34_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HDI5917563.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            11671..12228
                     /locus_tag="POB34_000562"
     CDS             11671..12228
                     /locus_tag="POB34_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDI5917564.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            complement(12218..12958)
                     /gene="cysQ"
                     /locus_tag="POB34_000563"
     CDS             complement(12218..12958)
                     /gene="cysQ"
                     /locus_tag="POB34_000563"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HDI5917565.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            13148..15091
                     /gene="cpdB"
                     /locus_tag="POB34_000564"
     CDS             13148..15091
                     /gene="cpdB"
                     /locus_tag="POB34_000564"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HDI5917566.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(15220..15600)
                     /locus_tag="POB34_000565"
     CDS             complement(15220..15600)
                     /locus_tag="POB34_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDI5917567.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            15689..16549
                     /gene="qorB"
                     /locus_tag="POB34_000566"
     CDS             15689..16549
                     /gene="qorB"
                     /locus_tag="POB34_000566"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDI5917568.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKSVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            16657..17622
                     /locus_tag="POB34_000567"
     CDS             16657..17622
                     /locus_tag="POB34_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDI5917569.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLIRQQMPPLMTLSGIALLVVGVVIAVRAKPEKPLTESVSES"
     gene            17730..18392
                     /gene="ytfE"
                     /locus_tag="POB34_000568"
     CDS             17730..18392
                     /gene="ytfE"
                     /locus_tag="POB34_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HDI5917570.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLSRAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(18639..19706)
                     /locus_tag="POB34_000569"
     CDS             complement(18639..19706)
                     /locus_tag="POB34_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917571.1"
                     /translation="MAILHPQECWLLERIMSPEYYRRRFEGWQEFVELCERQVAEWSK
                     TMPLDVRRRPLCEQIDVVWGGRVLPNIRSTLKSVQYDFIQLQQGDLRVLQSGGNISSD
                     MKGLIDYPSDWMSLAAQKQYDRLKWRGAHYNNLIRRTSGGYWYDGELTYYYEESLHGP
                     LALPMQLPLYELDSSVYLREDDSVTVAGLYLPDIPDASAQLLYRSEHIPEAWQGRVRT
                     KYVNEAGIQEYYWESGAWAKCNWKRIRRVANRFINVPPEGFFPQGMPEELYNWPQREA
                     QYVTDRQRIAAFSGEACPHSGEWSIFVEGRQATVTLEQGEQMPEWTDRKMEGEYKRGE
                     KFHVLWSLMNRHDGGSVWVEA"
     gene            complement(19805..20239)
                     /locus_tag="POB34_000570"
     CDS             complement(19805..20239)
                     /locus_tag="POB34_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917572.1"
                     /translation="MAKHSWLLIAALPLSISPSWGADFYYRQQEKGTVYVAEQKGEKD
                     EILSELPGVNFSRLWRIANLANNQETRLLSDFNPDKFDCDDRNNCQHTWLTDGRSVLW
                     SGKVLKNPPGEPNVDAASFQAFGAFAADKRSVYFDGQRTDDR"
     gene            complement(20506..21909)
                     /gene="cycA"
                     /locus_tag="POB34_000571"
                     /gene_synonym="dagA"
     CDS             complement(20506..21909)
                     /gene="cycA"
                     /locus_tag="POB34_000571"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HDI5917573.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVIVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRMAK"
     gene            complement(22218..22838)
                     /gene="fklB"
                     /locus_tag="POB34_000572"
     CDS             complement(22218..22838)
                     /gene="fklB"
                     /locus_tag="POB34_000572"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDI5917574.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            23057..23695
                     /gene="ytfB"
                     /locus_tag="POB34_000573"
     CDS             23057..23695
                     /gene="ytfB"
                     /locus_tag="POB34_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HDI5917575.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(23679..24326)
                     /locus_tag="POB34_000574"
     CDS             complement(23679..24326)
                     /locus_tag="POB34_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDI5917576.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKHNIFKPDIDPLQVNI
                     NIAALGGYYLINQYTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            24539..25330
                     /locus_tag="POB34_000575"
     CDS             24539..25330
                     /locus_tag="POB34_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HDI5917577.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDLPDADSVE
                     KLHITQQECIAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            25340..26182
                     /locus_tag="POB34_000576"
     CDS             25340..26182
                     /locus_tag="POB34_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDI5917578.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIAHQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            26192..26968
                     /locus_tag="POB34_000577"
     CDS             26192..26968
                     /locus_tag="POB34_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDI5917579.1"
                     /translation="MSDQPVLFSRVAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEIEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            26978..28519
                     /locus_tag="POB34_000578"
     CDS             26978..28519
                     /locus_tag="POB34_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDI5917580.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DAFFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHITQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            28516..30597
                     /locus_tag="POB34_000579"
     CDS             28516..30597
                     /locus_tag="POB34_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDI5917581.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDMAQLVAPT
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARIILDRDGNSIP
                     QTRNGFCDAWRFRSERTEDKKDKLLIHYCNGIFHVRETPVREFPIIRGIWFEKNWPGF
                     LNGTIYQPQDE"
     gene            30618..31094
                     /locus_tag="POB34_000580"
     CDS             30618..31094
                     /locus_tag="POB34_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDI5917582.1"
                     /translation="MRNIIRSINALLAALNITILAIIVACVTWQVAARFIFTSPSIFT
                     DELSRLLFICLGLFGGAYTAGQNRHLAIDLLPMMLKGKARRHLFLCIQIIVIIFATII
                     MVYGGGLLTMDTFDSGQTSPALGWQMGYIYMSIPISGVLIIIYTIDMVLTELKQPL"
     gene            31137..32438
                     /locus_tag="POB34_000581"
     CDS             31137..32438
                     /locus_tag="POB34_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDI5917583.1"
                     /translation="MDYWLPIIVLFGAFFFMLALGVPIVYAIGLSTLASISTQLDFNS
                     ALSVVSQKLASGLDSFTLLAIPFFILSGNIMNHGGIARRLINFARILGGRLPGSLAHC
                     NILANMLFGAISGSAVASAAAMGGVMHPQQVKEGYDPAFSTAVNVASAPTGLLIPPSN
                     TLIVYSLVSGGTSIAALFLAGYVPGILLGLALMVIAGIIAVRRGYPKPERPTLRQAGV
                     AIWMAIPSIFLIILIMGGVLSGIFTPTEASAIAVIYTLFLALVLYREISVKDLPKIFL
                     ESVITTAIVLLLIGSSMGMSWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIG
                     TFMDMTPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVS
                     KLPINKIIKPMLPFYAVMVLVLAMVTYIPQISMALPRALGY"
     gene            32491..33462
                     /locus_tag="POB34_000582"
     CDS             32491..33462
                     /locus_tag="POB34_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDI5917584.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKSKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(33574..34023)
                     /gene="rplI"
                     /locus_tag="POB34_000583"
     CDS             complement(33574..34023)
                     /gene="rplI"
                     /locus_tag="POB34_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDI5917585.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(34065..34292)
                     /gene="rpsR"
                     /locus_tag="POB34_000584"
     CDS             complement(34065..34292)
                     /gene="rpsR"
                     /locus_tag="POB34_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDI5917586.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(34297..34611)
                     /gene="priB"
                     /locus_tag="POB34_000585"
     CDS             complement(34297..34611)
                     /gene="priB"
                     /locus_tag="POB34_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HDI5917587.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQVITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(34618..35013)
                     /gene="rpsF"
                     /locus_tag="POB34_000586"
     CDS             complement(34618..35013)
                     /gene="rpsF"
                     /locus_tag="POB34_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDI5917588.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            35340..35615
                     /locus_tag="POB34_000587"
     CDS             35340..35615
                     /locus_tag="POB34_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDI5917589.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(35744..36430)
                     /gene="ulaF"
                     /locus_tag="POB34_000588"
     CDS             complement(35744..36430)
                     /gene="ulaF"
                     /locus_tag="POB34_000588"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HDI5917590.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGKYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(36430..37284)
                     /gene="ulaE"
                     /locus_tag="POB34_000589"
     CDS             complement(36430..37284)
                     /gene="ulaE"
                     /locus_tag="POB34_000589"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HDI5917591.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(37294..37944)
                     /gene="ulaD"
                     /locus_tag="POB34_000590"
     CDS             complement(37294..37944)
                     /gene="ulaD"
                     /locus_tag="POB34_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDI5917592.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(37958..38422)
                     /gene="ulaC"
                     /locus_tag="POB34_000591"
     CDS             complement(37958..38422)
                     /gene="ulaC"
                     /locus_tag="POB34_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HDI5917593.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(38432..38737)
                     /gene="ulaB"
                     /locus_tag="POB34_000592"
     CDS             complement(38432..38737)
                     /gene="ulaB"
                     /locus_tag="POB34_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HDI5917594.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            complement(38753..40150)
                     /gene="ulaA"
                     /locus_tag="POB34_000593"
     CDS             complement(38753..40150)
                     /gene="ulaA"
                     /locus_tag="POB34_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDI5917595.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNIMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            40505..41569
                     /gene="ulaG"
                     /locus_tag="POB34_000594"
     CDS             40505..41569
                     /gene="ulaG"
                     /locus_tag="POB34_000594"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDI5917596.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            41677..42432
                     /gene="ulaR"
                     /locus_tag="POB34_000595"
     CDS             41677..42432
                     /gene="ulaR"
                     /locus_tag="POB34_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HDI5917597.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(42429..43178)
                     /gene="yjfP"
                     /locus_tag="POB34_000596"
     CDS             complement(42429..43178)
                     /gene="yjfP"
                     /locus_tag="POB34_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDI5917598.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGAGYFTSLARSLFPPLIPETTA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            43360..43689
                     /gene="bsmA"
                     /locus_tag="POB34_000597"
     CDS             43360..43689
                     /gene="bsmA"
                     /locus_tag="POB34_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HDI5917599.1"
                     /translation="MFSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            43838..44113
                     /gene="yjfN"
                     /locus_tag="POB34_000598"
     CDS             43838..44113
                     /gene="yjfN"
                     /locus_tag="POB34_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HDI5917600.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(44230..45855)
                     /locus_tag="POB34_000599"
     CDS             complement(44230..45855)
                     /locus_tag="POB34_000599"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HDI5917601.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELRREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(45939..47102)
                     /locus_tag="POB34_000600"
     CDS             complement(45939..47102)
                     /locus_tag="POB34_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDI5917602.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(47105..47743)
                     /locus_tag="POB34_000601"
     CDS             complement(47105..47743)
                     /locus_tag="POB34_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HDI5917603.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPHNPFEPRRNRYAQKYLTLVL
                     MGGAVFLIVKGWSESSDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNLTQQNCQSKYENCYYDNVEQSWIPVISGFLLSRVIRKDRDEQFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(47753..48151)
                     /locus_tag="POB34_000602"
     CDS             complement(47753..48151)
                     /locus_tag="POB34_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HDI5917604.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(48169..48828)
                     /locus_tag="POB34_000603"
     CDS             complement(48169..48828)
                     /locus_tag="POB34_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HDI5917605.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(48879..49577)
                     /locus_tag="POB34_000604"
     CDS             complement(48879..49577)
                     /locus_tag="POB34_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HDI5917606.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(49596..49997)
                     /locus_tag="POB34_000605"
     CDS             complement(49596..49997)
                     /locus_tag="POB34_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HDI5917607.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(50124..50855)
                     /gene="rlmB"
                     /locus_tag="POB34_000606"
     CDS             complement(50124..50855)
                     /gene="rlmB"
                     /locus_tag="POB34_000606"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDI5917608.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(51035..53476)
                     /gene="rnr"
                     /locus_tag="POB34_000607"
     CDS             complement(51035..53476)
                     /gene="rnr"
                     /locus_tag="POB34_000607"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDI5917609.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(53515..53940)
                     /gene="nsrR"
                     /locus_tag="POB34_000608"
     CDS             complement(53515..53940)
                     /gene="nsrR"
                     /locus_tag="POB34_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HDI5917610.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(54145..55443)
                     /gene="purA"
                     /locus_tag="POB34_000609"
     CDS             complement(54145..55443)
                     /gene="purA"
                     /locus_tag="POB34_000609"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDI5917611.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(55547..55744)
                     /locus_tag="POB34_000610"
     CDS             complement(55547..55744)
                     /locus_tag="POB34_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HDI5917612.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(55826..56830)
                     /gene="hflC"
                     /locus_tag="POB34_000611"
     CDS             complement(55826..56830)
                     /gene="hflC"
                     /locus_tag="POB34_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HDI5917613.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(56833..58092)
                     /gene="hflK"
                     /locus_tag="POB34_000612"
     CDS             complement(56833..58092)
                     /gene="hflK"
                     /locus_tag="POB34_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HDI5917614.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(58178..59458)
                     /gene="hflX"
                     /locus_tag="POB34_000613"
     CDS             complement(58178..59458)
                     /gene="hflX"
                     /locus_tag="POB34_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDI5917615.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(59535..59843)
                     /gene="hfq"
                     /locus_tag="POB34_000614"
     CDS             complement(59535..59843)
                     /gene="hfq"
                     /locus_tag="POB34_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDI5917616.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(59929..60879)
                     /gene="miaA"
                     /locus_tag="POB34_000615"
     CDS             complement(59929..60879)
                     /gene="miaA"
                     /locus_tag="POB34_000615"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDI5917617.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(60872..62719)
                     /gene="mutL"
                     /locus_tag="POB34_000616"
     CDS             complement(60872..62719)
                     /gene="mutL"
                     /locus_tag="POB34_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDI5917618.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQYGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(62729..64066)
                     /gene="amiB"
                     /locus_tag="POB34_000617"
     CDS             complement(62729..64066)
                     /gene="amiB"
                     /locus_tag="POB34_000617"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HDI5917619.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(64085..64546)
                     /gene="tsaE"
                     /locus_tag="POB34_000618"
     CDS             complement(64085..64546)
                     /gene="tsaE"
                     /locus_tag="POB34_000618"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDI5917620.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(64518..66065)
                     /gene="nnr"
                     /locus_tag="POB34_000619"
     CDS             complement(64518..66065)
                     /gene="nnr"
                     /locus_tag="POB34_000619"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDI5917621.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            66064..67203
                     /gene="queG"
                     /locus_tag="POB34_000620"
     CDS             66064..67203
                     /gene="queG"
                     /locus_tag="POB34_000620"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDI5917622.1"
                     /translation="MSEPLDLNHLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(67186..67239)
                     /locus_tag="POB34_000621"
     CDS             complement(67186..67239)
                     /locus_tag="POB34_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917623.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(67473..67548)
                     /locus_tag="POB34_000622"
     tRNA            complement(67473..67548)
                     /locus_tag="POB34_000622"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67513..67515),aa:Gly,seq:gcc)
     gene            complement(67584..67659)
                     /locus_tag="POB34_000623"
     tRNA            complement(67584..67659)
                     /locus_tag="POB34_000623"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67624..67626),aa:Gly,seq:gcc)
     gene            complement(67696..67771)
                     /locus_tag="POB34_000624"
     tRNA            complement(67696..67771)
                     /locus_tag="POB34_000624"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67736..67738),aa:Gly,seq:gcc)
BASE COUNT        16428 a        18255 c        16762 g        16557 t
ORIGIN      
        1 cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct tcttctctaa
       61 ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca gcttttcagg
      121 catggtgccg tcttttttgt atttctccag cgcgtcaaaa gcgggtcccg ccatattcgg
      181 cgtcagttca acactggcgt tcgcttcgcc atcaatcatc gctttgtaga tgtccggtac
      241 accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt ctttaattgc
      301 ctgaattgcg ccaatcacca tgtcatcgtt atgggcgtaa accatgcaga tgtttttgcc
      361 gttgttttcc gctttgataa agctttccat gacttctttg cctttactgc gggtaaagtc
      421 acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt cggcaaagcc
      481 tttcttgcgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca ccacgttgca
      541 tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt cgaggatgtt
      601 gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg aacggtcgag
      661 caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc aacctgtcgc
      721 gaccaccgga gcgataaaga tcgcatccac cccttgcgcg acgaaggaac gtaccgcttt
      781 aatctggttt tcctgctttt gctgaccatc ggcaattttc agcgtaattc cgcgtttttc
      841 ggcctcactt ttcgccacat tggtttctgc agcgcgccag ccggattccg atccgacctg
      901 cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg ccgagactgc
      961 agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat tattattggt
     1021 gaaaagatgt tcgcggaaaa actatagaca attcgttatg taacggattg cgttacatca
     1081 cacttcataa aagtaaataa aacgttaatc acaagtttgt aatcgctttc atctcactat
     1141 gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa aactggcgaa
     1201 aaaggaaaat gaagacgaaa acaagcgaag acattcggcg cgagttggct ataatactcg
     1261 gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa cgtccctgcg
     1321 ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa cgcagatccg
     1381 atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt catgtccacc
     1441 gcgatgttct atccgtgcaa ctacggctat atcaaccaca ctctgtctct ggacggtgac
     1501 ccggttgacg tactggtccc gactccgtac ccgctgcagc cgggttctgt gatccgttgc
     1561 cgtccggttg gcgttctgaa aatgaccgac gaagccggtg aagatgcgaa actgattgct
     1621 gttccgcaca ccaagctgag caaagaatac gatcacatta aagacgttaa cgatctgcca
     1681 gagctgctga aagcgcagat cgctcacttc ttcgagcact acaaagacct cgaaaaaggc
     1741 aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga aatcgttgcc
     1801 tccttcgagc gcgcaaagaa taaataagtt cttatagcgc aataaccctg aacaccgggc
     1861 ttcggttagt aagggttttt ttatgcccgc gataaataaa cacaccttat tccaccaccg
     1921 cctgcaagcg taataacgcc tcttccacca cctcatccgc agccagacca atacgctttg
     1981 ccagccgggc gcgtagatcc atcatccgca cctgattcac cagcaccacg ccgtgcacat
     2041 cgccttcttc gcaatgtaaa ggaacgctaa atccggcata acgggcaaaa tttccgccct
     2101 gcgtaatggg ggccaccagc gtcattccca gttgattaaa ggcttgaacg gagagcacaa
     2161 gcgcaggtcg accagcacct tgctgttcat ggccgcttgc tggatcaaag ccaaccagca
     2221 caatgtctcc ccgttcaaat tcacttttct ttaccatatt tcgtcacccg caggggtgga
     2281 tttaccccag acatcctgct cgctaagttc cgcggcgttc atgtcacact gtgccagcag
     2341 ttcatcaagc gagtagcgcc tggagatggg tgtcagaatc agttggttgt tgctcacctg
     2401 cgcttccacg ctctgccccg gctgtaagtt aagttctttc attacgatat tgggaatgac
     2461 catacctgca ctgttccccc atctttttat ggtaatacgc atctgcacct ccttaagtta
     2521 tacaaagtat aactcacggt gctacaggaa acgttttacc cctgcacatt tgtgaacgcc
     2581 agcagagttt tttatgcgat tgcagatatg gaaagcggct cggagagaaa agcaaaagat
     2641 ggaaaagtta caaaaaaaaa tcgcgagata catcgcataa aacaacgcca cccgaaggtg
     2701 gcgtgctttt attattactt atccctgtct aaccagtcgc cgctttcaat tagctttaat
     2761 ccatcgacgg gtcgttgata aacgtacatc catgcactcc cgtacggcgt ctgaatcaac
     2821 tggcgcgcgt attcaccgcc cctggtgcgc aaggcatcaa gttcggccag cgtggcgttg
     2881 tcaatacgat aaacttcacc gtgtactgtt ccgttccccg gaactgcgcc tggatagtgg
     2941 cccaggctat acaactggta gttatcgata ctgaaatcgc ccagtaactg ggcattggtc
     3001 atccagtgac tgttgccttg tttgtggcgt aaactgccgt agacaaatat tcgcattgct
     3061 aaaactcgaa ctgatagagc aaatccagtg cctggtctac accagacacg gcttccagat
     3121 atagcttagg catcaggcga taacgtaacg tgagtgttgc tatagagtca aatataccca
     3181 cgccgtattt cacttgcaga cctggcaata catagccgct gaccactacc tgggaggagt
     3241 cgcctactcc ctgggtgtcg agcgctaaat tgcttacgcc aaacgtctcg ccgattttac
     3301 ccacaatctg gccactttgc gcaaccccca ggccaatcag cattgaggtc atcgccgcac
     3361 tgtcgctctg atcgctctcc agcccttgtc cacgtagcaa ataagacaat gcagcttgtt
     3421 gcgacatagc cgggtcagag aagatctccg ctttcggttc gtccgccaga ccagtgacgc
     3481 gaactccggc gattacgtcg tcttctgtag catccgggtt acgaatagct tcaatattaa
     3541 gatacggttg atctggcgga ccagagaaca gtagctcacc cttacgcaca atcagatcct
     3601 gaccataggc atggaagcgc ccttcaggga tgttgatctg cccgttcagg cccagccctt
     3661 gtttgtcctg aacgacgtta agatcgcccg tcagccgcgc tttcaggcca aaggcgtcaa
     3721 tgcgcacatt gttgccaacg tggacaatca ggttactgtt aatcggaatc gacgccgttt
     3781 tcggctcttc cggttgcagg ttatcgttaa gcatcaccac atcgctggag acgcctactg
     3841 cgctttccgg cagatcgtgc accacgatgc gcgcccacgg gacatccacg cgaccatcga
     3901 gggtaaacag gtttggtgta gcctcgaata caacatctgg cgatacatcc attcgtacca
     3961 tcggcggcac ggtgatccgc actttactgc ctttcgccgt tactcgtgcc cgccagtttt
     4021 caatttggct ccagtcggca tcaccgttca ggtagatttc accctgctgg gtccgtactg
     4081 taccggcaag cgtcgagcgc ataccgttaa agttgaccgc aagctggctc ggctgcatat
     4141 caaacggcat aaagttgccg tcgatatcca cacccgtaac ctgaagctga ccaaacaact
     4201 gcgggctttg cacatcacca cccagacgca agttggcact caccatcccc gctgcttttt
     4261 ccccacgggt aaagatgggg tttatcatcg caaggttgaa gttacggata ttgacgttgc
     4321 caccaagatt acggcggcct tgcggatcgg tcacctgcac ctgtccatca aactggccgt
     4381 tattggtcag gcggatggtc cagcccaatt cggcacggtt gttacgcaat tccgccgtca
     4441 gattcagtgt ctgaaacgcc accggcagcg ccgcatcgtt gacggtttgc gttacctgca
     4501 cgttacgccc cgaaagggtg atactgccct gcggcagccc ctctttcgtg gtgtcccagg
     4561 caacatccgc tttacccgtg aagataccgc tggcctgagt ggtttctggc ataaacggtt
     4621 tcagcatggc gaggtcgaag cggttgagat tcaccaccgc acgcccttcg gcccccgcat
     4681 cgatagtttg cggcacgcac agttccgcat tcgggttaag ccagcagtgt ggcccgatgc
     4741 tgattttttg ctccttattg cggtaatcca gcgcaatatc gcgggtcagc gaccacgggc
     4801 cgaccggcgt ctggaagcgg gtattgctaa gagttccctt ccagcgttct tctttgcgat
     4861 caaaacttcc tgccagatta agctgcccgg agacaggctc gccctgaatc cgcaactgta
     4921 gctcgtgctg cttttcgctg cctttggcat tcagggtgac gaggttgata tttacatccg
     4981 gctgcgaaat ttgctcaacg cgtacgtcga gtttcccggc gatctgatcg gtggatttaa
     5041 tgtcgccttc cacgcgaacc tgcgccacgg aaagttcctg ccagcgcagg ccgcgcgcgg
     5101 tgatatctgc cagtagttgt ggcgcttcca ccgtgccgcg tactttcacc agccctttcg
     5161 ctgtaccgcc aagccccggc agcgcgttat ccagccccgg cgcgttgatg gtggcatcaa
     5221 gattgagatc ttttaccccc agctcgcctt tcacttcggc actgtttggc ccgagttcca
     5281 gatgaagccc agggatcatc cactgcatat aactgttgcc tttcagcgtg ccgtcaacgt
     5341 tcactttgtt ctgtttgacg ttaccggtca gcttcagttc tggcacctcc atctgccagg
     5401 tgccgccgta caggctaccg cgggttttaa tcaagccatt gagtttcgac ggccactccg
     5461 ggaactcttt ggcggtgtta atgccattaa gcgttagctc accgcgccaa ctaatggcct
     5521 gctgccagtc gagcaacgcc ttgagttcag ttttcccttc cagcgccgcg acggtgagtt
     5581 tgtcgagatt gacctgctgt tcattacctt tggcatcaag ggtaatggtg gctggcggga
     5641 tctcctgtcc cttcactgcc gtacgcatag agagcgtgta atcggtcatt ttgccggtaa
     5701 gtttcagttt cagatcatcc gcctgatact gcttctcacc agtgaacggc cagtaaagct
     5761 gtttgctgtt cacttccacg ttgagcggca atccggcttc cgccagtcgc gtctgggcgc
     5821 gtaaatccat atccaccgga ccggaaagat taacgccaat ctccagctgt tcgcgcagcg
     5881 cgccgcccac ttttagcttc actttttcac ctttcaacgg ctccacgttc agtgtgctgt
     5941 tgagggtgat atccaccggc cagttgtctg acagctgcgc cgtgccgctg gcgttgacga
     6001 tcccctggtt ggaatcgata tccagggcgt ccagtttagt attgccgtca atgctgctca
     6061 ctttcagcag catggtgcgc acggtgatgt ccgtgtcgcc cgtcacgcgt aactgctcgc
     6121 ctttaaactc ttcaatgttc aggttaagcg gcaaatgcaa gtcggtcatt tccggcaata
     6181 ccgggcgaga aaagagatct ttcagcgttt cgccgagcgg cttttcctcc ggctgcggat
     6241 tttcaatttt cggttcgacc acttcttcct gcgccacttc cgccactttc ggcagagcaa
     6301 tcagcaggcc tttcagcgac gtcggtttca gggtcagggt tttctcctgc cagttcaggc
     6361 cggaggtgaa gtccatcacc gacaccgtgg tgtcatcaat cttgatgttg acgttgtcca
     6421 gcgccacccg tgtcagggtg atgggatacg gcgtggagag atccagcgga ccgctatctt
     6481 cctcttcttc aacctgttca gaaggaggca tttttttact gtcgatgttg acctgaatgt
     6541 ctttcagcgc caggtcatta atacaaacgc tactgttcca caggcactca agaccgaccg
     6601 ccagatgcag attacccgct tttaccgcca cgcctggctg ctcataacga acgtcagaca
     6661 aggtgagatc gcgccagccg ccggtgacct tgccaatatc cagtcctggc acccagcgat
     6721 ccgccgcttt aaataccaga tgcaggccgc tggtggtgcc caccagaaac gccaccgatc
     6781 ccagcaacag taagataacg ataaccacgc cgaggctgat ttttttccat aaactcataa
     6841 ttctggcccc agaccgatgt aaaactgtaa cccgtgctcg tctttatccg cgaccggtac
     6901 ggcaaaatcg agtttgattg gcccgaccgg cgattcccag cgtacgccga ccccggtacc
     6961 ggttttaaag tcgctgcggc gaatatcgct taccgcttcg ccactatcga caaacaccgc
     7021 gccccaccat tttccggtca cgttgtactg gtattccagc gatccggtta tcagcttcga
     7081 ggcccctttc aggtcaccgt tggcgtattt cggggcgata gatttgtatt tgtagccgcg
     7141 aatactgcga tcgcccccgg cgaagaaacg cagatccggc ggtactttgt cgaaatcacc
     7201 cgtttcaatc cagcccagcg tgccgcgtgt cacaaaacga tggcgatcgt acagtgtgcg
     7261 gatccagacg ttctgcgcct ggaaaacgga gaaatcgaca tctgagcccc aggccgtgtt
     7321 ggagtagtcg atagagtagc gttgcgagtc gccccaggtt ggcatcaggc caccacgaga
     7381 acgcgtgcgg ctaatcatca ccccaggata aaacagcatc gtggtgttgg taatttcacc
     7441 ctgggtaaag tggtcgaggc tccagcgcag gttaatggca cgctgccagc cgctggagag
     7501 atcccagtag cgagaagcca ccagcgtagt ggagtcagat tcggtatcgt tcaggtcagt
     7561 gcgcttaaaa ccgccctgca ccaaataata ttgttccagt ggattcttca gcagcggcat
     7621 tttatagctg aagtcgaggg tctgttccgg cgcggaaata ctggtactgg tggtcagact
     7681 gtgaccataa gagttcatcc acggcttttt ccacgtcgct ttcacgcgcg gtcccacgtc
     7741 cgtagagtaa ccgaccccgg tttcgatggt gttttcagtt cgcggcgaaa ccacgcccgt
     7801 caagggtaat actttcgttt cgcgcgcttt atcaaattgt ggagccacca ccaccgagtt
     7861 aaaccagccg gtagcagaaa gtcggcggtt cagttccgcc agatctttcg attcgtactc
     7921 atcgccctct ttaaacggca ccagattttg caggtattca tcgcggattt gtgatccttc
     7981 aaaggtcaca tgcccaaagc ggtaacgttc gccactgtta taatcaatat cccagaaggc
     8041 tttatgcagg ccgagcgcaa tgcccagctg cgctttggta aattcgctat cgaaataacc
     8101 tttacgcaac gcaatgctgg ttaaggactt tttgaaattt tcataatcgc cctggttcag
     8161 caccgtgcca atagccgggc gagtatcgag caatttcaaa tagtctttat cggtccgcgc
     8221 gccgccgcgc aataccacat cggtgccgcc aattaacacc ggcacacctg gcgtgacttt
     8281 ggcgatcaat acctgccgcc ctttctttgg cggtggacgg agatcaaatt caatggtcgg
     8341 ctggtaataa cccagcgctt tcagaccttc acggatggca tcatcgacgc gtgcgcgaaa
     8401 gcgacggtct ggcgtcactt catcactttc aatcgtagaa agctgcgcac gaacgttctt
     8461 ttccagctgt cccgataacc cctcgacctg tagacggacg ttcgcggcga cggcagatcc
     8521 gcttaagcag agtaagctta cacagcataa ctgtcggata tagcgcacat tttctcctga
     8581 atatcctttt ttcctgcccc tggaacgccg ttaaacggct taacaaaaat ccagtaatat
     8641 ggattaaaaa gcagactaaa ccccaaatat ttcttatgtt ttactttaga cctattcacg
     8701 gtggttattg tgtgcaaata cgcctcttgt tacaacctta accccaatga ccgattttcg
     8761 ggagagcgac accatgagtt tatttgataa aaagcatctg gtttcccccg ccgatgccct
     8821 gcctggacgt aacaccccga tgcccgtagc cacgctgcat gcggtcaacg gtcactcaat
     8881 gaccaatgta cctgatggaa tggagattgc catttttgcg atgggttgtt tctggggtgt
     8941 ggagcgtctg ttctggcagt tacccggcgt ttacagcacc gccgcaggct ataccggagg
     9001 ctatacgcca aatccgactt atcgggaagt gtgctccggt gatacgggtc atgccgaagc
     9061 ggtacgcatt gtttacgatc cttccgtcat cagctatgag cagttgctac aggtattttg
     9121 ggagaatcac gatcccgccc agggaatgcg tcagggcaat gaccacggca cgcagtatcg
     9181 ttcagcgatt tatccgctga ccccagaaca ggatgccgca gctcgcgcca gtctggaacg
     9241 ttttcaggcg gcgatgcttg ccgccgatga tgatcgtcac atcaccacgg aaatcgctaa
     9301 cgccacaccg ttttattatg ccgaagatga ccaccagcaa tatctgcata aaaacccgta
     9361 tggttactgt ggaattggcg gaattggcgt ctgtctgcca ccggaagcat agcgttacgg
     9421 gtacaaatgt agattgttga taaagtgcgc tttatttatg ccagatgcgg cgtaaacgcc
     9481 ttaccaggcc tacaaaatcg tccaaattca atatattgca aggactgcgt aggcctgatc
     9541 ggcatagcgc atcaggcagt attgcattta tcagcagtct gaattttaac cctctggcga
     9601 ctttacagta ccttacgcta tactagccac tgaaaatgcc ggttcacttt cttcgaatcg
     9661 gctttcaatg tgtatttcac acaaattaat caacttcccc ttccgaggat ctggcctgaa
     9721 aggtcggata agatatgtta aacagtattt tagtcatact ctgcttgatc gctgtaagtg
     9781 cgttcttctc gatgtccgag atctcacttg ccgcctcacg caaaatcaaa cttaaactgc
     9841 tggctgatga aggcaatata aatgcccaac gcgttctgaa tatgcaggaa aatcccggca
     9901 tgttctttac cgtggtccaa atcggtctga acgcagtggc gattctcggc ggtatcgtcg
     9961 gtgatgcggc attttctcca gcttttcaca gcctgttctc ccgctatatg tcggcagagc
    10021 tctctgagca actgagcttt attctctctt tctcgttagt gactggcatg tttatcctgt
    10081 ttgcggattt aaccccgaaa cgcatcggta tgattgcgcc agaagctgtc gctttgcgta
    10141 tcatcaaccc gatgcgcttc tgcctgtacg tttgcacccc gctggtgtgg ttcttcaacg
    10201 gcctggcgaa catgatcttc cgtattttca aactgccaat ggtacgtaaa gatgacatca
    10261 cttctgatga catctacgcg gtagtggaag ccggtgcgct ggcgggcgtg ttacgtaaac
    10321 aggaacacga gctgattgaa aacgtctttg agctggaatc ccgtaccgtt ccgtcttcaa
    10381 tgacaccgcg tgaaaacgtg atttggtttg atctccacga agatgagcaa agtctgaaga
    10441 ataaggtggc ggaacatccg cactctaagt tcctcgtctg taatgaagat attgaccaca
    10501 tcatcggtta tgtcgattct aaagacctgc tgaaccgcgt gctggctaac caaagcctgg
    10561 cactgaacag cggcgtacaa attcgcaaca cgctgattgt gccggatacg ttaacccttt
    10621 cagaggcgtt ggaaagtttt aaaaccgcag gtgaagactt cgcggtgatc atgaacgagt
    10681 acgcgctggt ggtggggatc atcaccctca atgacgtgat gaccacgctg atgggcgatc
    10741 tagtcggtca ggggctggaa gagcagattg tcgcccgtga tgagaactca tggctgattg
    10801 acggcggcac cccaattgac gacgtcatgc gcgtgctgga tattgacgag ttcccgcagt
    10861 cgggcaacta cgaaaccatc ggcggcttta tgatgtttat gctgcgtaag atcccgaaac
    10921 gcaccgattc ggtgaaattc gccggctaca aatttgaagt ggtggatatc gataactacc
    10981 gcatcgacca gctgctggtg acccggatcg acagcaaggc caccgccctt tcgccaaaac
    11041 tgcctgacgc taaagataaa gaagaaagcg tcgcgtaacc cccagaaaca tcaacggctc
    11101 ctgaatcagg agccgtctta ttactgcata gcactttggt taagccatct ctgtttgcag
    11161 acgcataacc tgacggttaa cttcggacat cactgacaaa tgcagcttat ccttcacttt
    11221 tgggataaga atcttaccct tatcaaattc aaaagcgcca acgtccttga tgtataaccg
    11281 tccacggaac aggatcttta cgtacttcgc cacctgaagt gggttgtacc gttggaaaat
    11341 tttcattgtt atctcctgct gagtattacg cctgtgcggt gccacaatcg gcccaactat
    11401 tatgaggcgc aaattttaat gcttagtgac tatagactat ccgggcaatg tttccaccgc
    11461 gtataacttt ttttacctaa aggttacaat tattcagaat tatcttttta ccgaagcgcg
    11521 gtcttcagta taagcattca tttttcatat gatttgtgcg cttgaccgca aactggcatc
    11581 acacttgcgg gaaattcgat aaatagcaca tatgattaaa actcagaccc aagtggtcgg
    11641 atcacctgca tatcataaga aggaaacacc atgaccctac gcaagattct ggcactcacc
    11701 tgcctgctgt tgccgatgat ggcttccgca catcagttcg aaaccggtca gcgagtgccg
    11761 ccgattggca ttaccgatcg gggcgagttg gtgcttgata aagatcagtt tagctacaaa
    11821 acctggaaca gcgcgcagtt agtgggaaaa gtgagagtac tgcaacatat tgctggtcgc
    11881 acctcagcaa aagagaaaaa cgcgacgctg attgaagcga ttaaatcagc gaagttaccg
    11941 cacgatcgtt accagaccac caccattgtt aacaccgacg acgcgattcc gggttccggc
    12001 atgtttgtgc gcagcagtct ggagagtaat aaaaagcttt atccctggtc gcagtttatt
    12061 gtcgatagca atggcgtcgc gcgcggtgcc tggcagctgg atgaagaaag ttccgctgta
    12121 gtggtgctgg ataaggatgg tcgcgtgcaa tgggccaaag acggggcgct cacacaggaa
    12181 gaggtgcagc aagtgatgga cctgctgcat aaattaatta ataaatagac actctgaacc
    12241 ccggattcag gaacgactcg cgcggagtgt aatccagcgg tttaccctgc cagtcgtgaa
    12301 cgtgcgctcc ggcagccgca gccacagcat gaccagcggc ggtatcccaa atattcgttg
    12361 gcccgaaacg cgggtacagc tgcgcctgtc cttccgccac caggcagaat ttcagcgaag
    12421 agccgatgga cgtggtctga tgttcgccaa gctgttgcag atactctttc agctccgcat
    12481 ctgcatggga acggctgata accaccagcg gcgggcgcgc atcgcggacc tgaatctgct
    12541 tgcgcacacc gcactcttct ttccaggcct tgccttctgc cgcgctgtac attacgttca
    12601 ttaccggcgc atacaccaca cctaaaatcg gtttgccatg gtcaatgagc gcaatgttaa
    12661 cggtgaattc gccattacgt ttaataaact ctttagtacc atccagcggg tctaccagcc
    12721 agtaacgctg ccagtgctga cggacttccc aaccgggagg atcttcttca gaaaggaccg
    12781 gaatatccgg tgtcagcgta cgtaaaccgt ccatgataac ggtgtgtgcg gcaatatccg
    12841 ctgccgttac cggagaattg tccgctttgc tgacgacgtc catcggtttc gtcccgtcgt
    12901 agacctgcat aatggcatcg cctgcattcc gtgcaagctg gcatacttga tctaacattt
    12961 ctccacctcg tctctgtgag cggtgttaac ttattgtttt acttataccc tatcgttaat
    13021 gaatgcgcca actgtgatag tgtcatcatt ttcaaagcgt aaaattgtgg cattcttcac
    13081 tgttctataa gtaagacgtt tattctccct tttctttcgt attcccgatg ataaaaggat
    13141 gtccctgatg attaagttta gcgcaacgct cctggccacg ctgattgccg ccagtgtgaa
    13201 tgcagcgacg gtcgatctgc gtatcatgga aaccactgat ctgcatagca acatgatgga
    13261 tttcgattat tacaaagaca ccgccacgga aaaattcgga ctggtacgta cggcaagcct
    13321 gattaacgat gcccgcaatg aagtgaaaaa cagcgtactg gtcgataacg gcgatttgat
    13381 tcaggggagt ccgctggccg attacatgtc ggcgaaagga ttaaaagcag gtgatgttca
    13441 tccggtttat aaggcgctga atacgctgga ttatacggtc ggtacactcg gcaatcatga
    13501 atttaactac ggtctggatt acctgaaaaa tgcgctggcg ggagcgaaat tcccttatgt
    13561 aaatgccaac gtcattgacg ccagaaccaa acagccaatg tttacaccgt atttaattaa
    13621 agacaccgaa gtggtcgata aagacggaaa aaaacagacg ctgaagattg gctatattgg
    13681 cgtcgtaccg ccgcaaatca tgggctggga taaagctaat ttatccggga aagtgacggt
    13741 gaatgatatt accgaaaccg tgcgcaaata cgtgcctgaa atgcgcgaga aaggtgccga
    13801 tgtcgttgtc gttctggcac attccgggct gtctgccgat ccgtataaag tgatggcgga
    13861 aaactcagtt tattacctca gtgaaattcc gggcgttaac gccattatgt ttggtcatgc
    13921 tcacgccgtt ttcccgggta aagattttgc tgatatcgaa ggggctgata tcaccaaagg
    13981 cacgctgaat ggtgttccgg cggtaatgcc gggcatgtgg ggcgatcatc ttggggtggt
    14041 cgacttacaa ctcagtaatg acagcggtaa atggcaggtg acgcaggcga aagcggaagc
    14101 tcggccgatt tacgacatcg ctaataaaaa atccctcgcg gcggaagaca gcaagctggt
    14161 agaaacactc aaagccgatc acgatgccac acgccagttc gtcagcaagc caatcggtaa
    14221 atctgccgac aatatgtata gctatctggc gctggtgcag gacgatccga ccgtgcaagt
    14281 agtgaacaac gcgcaaaaag cgtatgtcga acattacatt cagggcgatc cggatctggc
    14341 aaaactgccg gtgctttcag ctgccgcacc gtttaaagtt ggtggtcgca aaaatgaccc
    14401 ggcaagctat gtggaggtgg aaaaaggcca gttgaccttc cgtaatgccg ccgatcttta
    14461 tctctacccc aatacgctga ttgtggtgaa agccagcggt aaagaggtga aagagtggct
    14521 ggagtgctcc gccggacagt ttaaccagat tgatcctaac agcacgaaac cgcaatcact
    14581 catcaactgg gatggtttcc gcacctataa ctttgatgtg attgatggtg tgaattatca
    14641 gattgatgtt acccagcctg cccgttatga cggcgagtgc cagatgatta atgccaatgc
    14701 ggaaaggatt aagaacctga cctttaatgg caagccgatt gatccgaacg ccatgttcct
    14761 cgttgccacc aataactatc gcgcttacgg cggcaaattt gccggtacgg gcgacagcca
    14821 tatcgctttt gcttcaccgg atgagaaccg ctcggtgctg gcagcgtgga ttgctgatga
    14881 gtcgaaacgt gcgggggaaa ttcacccggc ggcagataac aactggcgtt tagcaccgat
    14941 agctggcgat aagaaactgg atatccgttt cgaaacttct ccgtcagata aagccgcagc
    15001 gtttattaaa gagaaagggc aatatccgat gaataaagtc gcgaccgatg atatcgggtt
    15061 tgcgatttat caggtggatt tgagtaagta aaacacttct ttttcggcct ataaatcatc
    15121 aaccgcatcc ggcatttatt ggcggatgcg atgctggcgc atcttatccg ccctacaagc
    15181 catgcaccgt agaccagata agctcagcgc atccggcagt tatgccgcac gttcatcccg
    15241 caccgccaac acctcgggca aattcaactc aatccagtcc gccagtgcag caaccttttc
    15301 gctcacctgc tcgcccagcg gcgtgaggct gtattccaca tgcggcggca ccaccggata
    15361 cgcgatacgg ttaagaaaac catcctgttc taacgcctgt aacgactgcg caagcatctt
    15421 ttcactcacc ccaccgattt tgcgccgcag gtcgctaaag cgatgagtac cttcgcgtag
    15481 cgccaccaga atcaataccc cccaacggct ggtgacgtgt ttcaacacct cgcgcgacgg
    15541 gcactgttcc gcaaagagat taccctcttt cagttgttgc gacaggctaa cctgactcat
    15601 ttcatactta cctttttgta cgtacttact aaaagtaagt ttaggtgtta gcgtatttaa
    15661 acacaagaca aaacgatgga gacttcccat gatcgctatt actggtgcca ctggccaact
    15721 tggtcactat gttattgaat ccttgatgaa aacggttcct gccagccaaa tagtggctat
    15781 cgttcgtaat ccggcaaaag cccaggccct ggcagcacaa ggcattaccg tgcgtcaggc
    15841 tgactacggc gatgaagccg cactgacatc tgcacttcag ggagtggaaa aactactgct
    15901 gatctcttcc agcgaagtgg gtcaacgtgc cccgcagcat cgtaatgtta ttaatgccgc
    15961 aaaggcggct ggcgtgaaat ttatcgctta taccagcctg ctacatgcag atacctcccc
    16021 gctcggcctc gccgatgagc acatcgagac ggagaaaatg ctggctgatt ctggcatcgt
    16081 ttacaccctg ctgcgcaacg gctggtacac cgaaaactac ctcgccagcg ccccggcagc
    16141 actggaacac ggcgtattta tcggtgcggc gggcgatggc aaaatcgcct cagcaacgcg
    16201 ggcagattat gcggcagctg cggcacgcgt gattagcgaa gccggtcacg aaggcaaggt
    16261 ttacgaactg gcgggcgata gtgcctggac gttgacacag ttagcggcag agctgaccaa
    16321 acagagcgga aaatccgtta cctatcaaaa tctgagcgaa gccgatttcg ccgcggcact
    16381 gaaaagcgtc ggactgcccg acggactggc ggatatgctg gcggattctg acgttggcgc
    16441 atcgaaaggt ggtctgtttg ataacagcaa aacgcttagc aaattgattg gccgcccaac
    16501 gacaacgtta gccgaaagcg taagccatct ttttaatgtt aataactagt taattaaagt
    16561 ggcatcctcc cgcatcctct ctgataatga cgggatgccg ggagcaatca tgtctgcttc
    16621 ctgaactttc ttctgacaga ccaatggatg ccagtaatga ttagcggcgt gctgtacgcc
    16681 ctgttagcag ggttgatgtg ggggcttatt tttgtcgggc cgttgatcgt accggaatac
    16741 ccggcgatgt tgcagtcgat ggggcgttat ctggcgttag ggttaattgc gctgcccatt
    16801 gcctggctgg gacgcgtgcg tctgcgtcag ttggcgcgtc gggactggct taccgccttg
    16861 atgctcacta tgatgggcaa cctcatttat tacttctgcc ttgccagtgc cattcaacgt
    16921 actggcgcgc ctgtttccac gatgattatc ggcaccctgc cggtggtcat tcctgtcttt
    16981 gccaatctgc tttatagcca gcgcgacggc aaactcgcgt ggggaaaact cgccccggca
    17041 ctgatttgta ttggcatcgg cctggcgagt gtgaatattg ctgagttaaa ccacggactc
    17101 cccgattttg actgggcacg ttatacctct ggcatcgtgc tagcgttagt ttccgtggtc
    17161 tgctgggcat ggtatgccct gcgcaacgcc cgctggctgc gggagaatcc cgacaaacat
    17221 ccgatgatgt gggcgacggc gcaggcgctg gtcacactgc cggtttctct catcggctat
    17281 ctcgtcgcct gttactggct gaatacgcaa acgccggact tctccctacc ttttggcccc
    17341 cgtccgctgg tgtttattag tctgatggtt gcgatagccg tgctttgctc atgggttggc
    17401 gcactctgct ggaacgtcgc cagccagcga ttaccgacag tgattctcgg gccgctgatc
    17461 gttttcgaaa cactggcagg tttgctgtac acctttttga tacgccagca aatgccgccg
    17521 ctgatgacgc tgagcggtat cgcgctgtta gtggttggcg tggtcattgc agtcagagca
    17581 aaaccagaaa aacccttaac tgaatctgtc tcagaaagtt gacatactgg ctgtgagtta
    17641 aataagcctc tgctacgtaa gggttatagc ttttgcctta aagatgcatt taaaatacat
    17701 cttatcttat taagaatgag gtatcagcta tggcttatcg cgaccaacct ttaggtgaac
    17761 tggcgctctc tattcctcgc gcttcagctc tgtttcgtaa atatgatatg gattactgct
    17821 gtggcggtaa gcagacgctg tcgcgcgcgg cggcacgtaa agaactggat gttgaggtca
    17881 ttgaagctga actggcaaag cttgctgaac aaccgattga gaaagactgg cgtagcgccc
    17941 cgctggcaga aatcattgac catatcatcg tgcgctacca cgatcgtcac cgcgagcagc
    18001 tgccggagct gatcctgcaa gcgactaaag tcgagcgcgt tcacgccgac aaaccgagcg
    18061 tgccaaaagg gctgacaaaa tacctgacta tgctgcatga agagctttcc agccacatga
    18121 tgaaagaaga gcagatcctc ttcccgatga tcaaacaagg catgggcagc caggcaatgg
    18181 ggccaatcag cgtaatggaa agcgagcacg atgaagcggg cgaactgctg gaagtgatta
    18241 aacacaccac caataacgtc acaccgccgc cggaagcctg caccacctgg aaagcgatgt
    18301 ataacggtat taatgaactg attgatgacc tgatggatca catcagcctg gaaaacaatg
    18361 tactgttccc acgcgcgctg gcgggtgagt aatttgttcg atcaacgcac ctggtttaca
    18421 caatcaggtg cgttttcaaa tgctttgagt tattcaccac tccgggacaa tcttttgtat
    18481 ctactgaact gggaaaattc attattcagc caataataag ttccttcaac ggttactaac
    18541 ttattttttt tattaatata attacttatt catcgctcct gtataagcca gaataaaatc
    18601 acccattccc tggtttttac gtgtacatcc agctaccgct acgcttccac ccacaccgac
    18661 ccgccatcat ggcggttcat cagcgaccac agcacatgga atttctcgcc tcttttatac
    18721 tccccctcca ttttcctgtc cgtccattct ggcatctgct ctccctgctc cagtgtcacc
    18781 gtcgcttgtc taccttctac aaaaatcgac cactccccgc tatgaggaca agcttcaccg
    18841 ctaaaggccg ctatccgctg ccggtctgta acatactgcg cttcgcgctg tggccagttg
    18901 tacaactcct ccggcatgcc ctgtgggaaa aatccctccg gcggcacatt gataaatcgg
    18961 tttgccaccc gccgtatacg tttccaattg cattttgccc acgccccgct ttcccagtaa
    19021 tattcttgta tcccagcctc gttgacatac ttcgttcgta cccgcccctg ccaagcctcg
    19081 ggaatatgtt cactccggta caacagttgg gcgctggcat ctgggatatc cggcagatac
    19141 aggccagcca ccgttaccga gtcatcctca cgcaggtata cgctactatc cagctcatac
    19201 aacggtaatt gcatcggcag ggccagcggg ccgtgcagac tttcctcata ataataagtc
    19261 agttcgccgt cataccagta tccgcccgag gtacgtctga ttaaattatt ataatgtgca
    19321 cctcgccatt tcagtctgtc atactgtttt tgcgcggcga gtgacatcca gtccgacgga
    19381 taatcaatta gtcctttcat atcactgcta atattgcccc cggattgcag tacccgcagg
    19441 tccccctgct gtagctgaat aaagtcatac tgtaccgact ttaaagtact gcggatattg
    19501 ggcaacactc gcccgcccca gacgacgtca atctgctcgc acagcggacg acggcgcaca
    19561 tccagcggca tggtcttgct ccactctgcc acctgtcgct cgcacagctc gacaaactcc
    19621 tgccagccct caaaacgacg ccgataatat tccggcgaca ttattctttc cagcaaccag
    19681 cactcctgtg gatgtaatat agccacttta tctcctgatt tattaattaa ttgtctattg
    19741 atacttttat ttcggtacat aaatatttgt ttgcaactga gacacagcta tatacatatc
    19801 cacttcatct atcatcggta cgctgaccgt caaaatagac gctgcgttta tcggcagcga
    19861 aagcaccgaa tgcctgaaaa ctggcggcat cgacattagg ttcaccgggc ggatttttca
    19921 ggactttgcc agaccaaaga acagagcgtc catcggtgag ccaggtatgc tggcaattat
    19981 tccgatcatc gcaatcgaac ttatcgggat taaaatcgga cagtaagcgg gtttcttggt
    20041 tattggctaa attggcaata cgccagaggc gggaaaaatt aacgcccggc agttcggaga
    20101 ggatctcatc cttttctccc ttctgttcgg caacataaac cgtgcctttc tcctgctggc
    20161 ggtaataaaa atctgcgccc caggaaggtg aaatggagag aggtaatgcg gcgattaata
    20221 accaactgtg cttagccata gtgaactctg caatgagtgg caaagatcca cggtaagccc
    20281 gcgcctgaga gctgtcgatg acgggaagta tttttaggaa tgtataaaga caaaggagat
    20341 tgctgacaaa gtgtgctttg ttcatgccgg atgcggcgta aacgccttac ccggcctaca
    20401 aaagcacgca aactcaatat attgcagagg ttctgtaggc ctgataagcg tagcgcatca
    20461 ggcaattttg cagcctgaaa gacacccgca ggtgccttag cggcattact tcgccatgcg
    20521 ttttttacca ataaacagcc agcccaaccc cagcgcgata aaccacagcg gggtaaccag
    20581 cagcgcctgg cgagtgtcat cttccagtgt cagcagcacg atcacgaaca caaagaacgc
    20641 catacatacc cagcacatca gcttgccgag cggcatcttg tagatcgact tctcatgcag
    20701 atgaggacgc tgtttgcgat acaccaggta cgagcaaagg ataatcgtcc agacgaacat
    20761 aaacagaatc gcggaaaccg tcgtgatcat cgtgaacgcg ccaatcacgc tgggattcac
    20821 atacagcatc accacgccgc cgagcagaca gatgcaggag aaggtcagcc ctttcgcggg
    20881 tactgcgcgc ttagagagtt tagcgaacgc tttcggtgca acaccttcct gggccaggcc
    20941 aaacagcata cggctggtag agaagacgcc gctgttagcg gaagacgccg cagaggtcag
    21001 caccacaaag ttgatcacgc tggcagccgc aggcaaaccc accaacacga acagttcaac
    21061 aaacgggctt ttctccggga ctaccgaact ccacggcgtc acggacataa ttacaatcag
    21121 cgcgaagacg tagaacataa tgatacggat cggaatagag ttaattgcac gcggcagtga
    21181 tttctccgga tctttggttt ccgcagcggt tgtacctacc agctcaatcc ctacgaaagc
    21241 gaaaaccgct atctggaatc cggcaaagaa gccacttaaa cctttcggga accagccgcc
    21301 atcattccac aaatgcgcga atgacgcttc cacaccagtc ggtgactgaa agtgcatcgc
    21361 caccataacc aggccgacga caatcagcga cacaatggcg acgattttga tcatcgcaaa
    21421 ccagaactcc atctcaccga acattttcac ggtggcgaga ttgagcgtca gcagcagcac
    21481 tatcaccgcc agcgaggcga cccagtcgga gagatcgggg aaccagaact gagcataagc
    21541 cgtgatcgcc accacgtctg ccataccggt tacaacccag cagaaccagt aagtccagcc
    21601 ggtgaaatat cctgcccacg gcccgagtaa atcggaagcg aagtcactaa aagatttgta
    21661 ttccagattc gaaagcagca attcccccat tgcccgcatc acgaaaaaga gcataaaacc
    21721 gatgatcata taaacgaaaa tgatcgacgg cccggcaagg ctaatcgttt tgccagaccc
    21781 cataaacaac cccgtaccaa tggcaccgcc aatggcaata agctgaatat gtcggtttgt
    21841 gagattgcgc cgtagcgact gttcagccgg agcctgatca tcggcaacga cttttacctg
    21901 atctaccatg tttttttctt cctgtacctg tctgtgttgt tcaggctcta cggcctttag
    21961 tcagtctatg atacgacgaa tctgtatcgg gactcatcga tattaggtaa caatacgcgg
    22021 gatgaatact aagatttaga tctaatgtta attttatgtt taaagtgagt gtcatatcac
    22081 ggtgataacg cgaaacagct cacaaaaata cacgcttacg gggtctttgc aacacaaaat
    22141 agcgccgttt ggcaaactga agggtttatt gctgaatgcc tgctcccctc tggttcgagg
    22201 ggaacagaat gcgctgctta gaggatttcc agcagttcga cttcaaacac cagggtgctg
    22261 aacggaggga tggatgcgcc tgcgccgcgc tcgccatatg ccagttcctg cgggatagtc
    22321 agttcccatt tagaacctac cggcatcaga gtcagtgctt caatccagcc agggatcacg
    22381 ccattaaccg gaaattcagc gggttcacca cgagcaacgg agctgtcaaa cacggtgccg
    22441 tcgatcagtt taccggtgta atgaacacga acgcggtcgg tacgtgccgg aattgcgcct
    22501 tcaccctggt tgatcacgcg gaattgcagg ccagattcgg tgctattcac accttctttt
    22561 ttggcgtttt cttccaggta tttcacacct tcagcagcca tcgcctggaa acgctgacga
    22621 cgaacggcat cggcgcgctc gtggatttca cgcagcgcgc gatgcaccac atcaaccgga
    22681 acagccggat gtttgccttc cagcgcatcg gcaatacctg caaccagtgc ttctggcagc
    22741 agcccttcca ggccagattc actcagttgt tgccctacct gcaaaccaat gccgtagctt
    22801 gcttgcgctt cgatggtgtc aaaagttggg gtggtcatcg tatttccttt cttagggtat
    22861 aaaagtagcg ggcagcatat cagccacact tctttgggta aaactttgtc gcaggaatga
    22921 tgacaaatcc cggggaaaaa gaaacaatag caatatcccg tatgaatata ataaaggcgg
    22981 gtttaccacg gacatcatag cgttagctta ttcataggct atgattgagg aacaagacgc
    23041 ggagcaggag gaaaccatgc ccgggcgctt tgaactaaaa ccaaccctgg agaaagtctg
    23101 gcacgcgccg gacaattttc gcttcatgga cccgctgccg ccgatgcatc gtcgggggat
    23161 cattattgcc gccattgtat tggtggtcgg atttctgctt ccatctgatg atacgcccaa
    23221 cgcgccggtg gtgacgcgtg aagcgcagtt agatattcag tcgcaatcac aaccgccgac
    23281 agaagaacag ctgcgggcgc aattagtcac cccacaaaat gatcctgatc aggtggctcc
    23341 ggtcgctcca gaacctatac aggaaggcca gccagaagag cagccgcaaa ctacccaaac
    23401 gcaaccattc cagccagata gcggaattga taatcaatgg cgctcttacc gtgtcgaacc
    23461 cggtaaaacg atggcgcagc tgttccgcga ccacggactg cccgctaccg atgtctatgc
    23521 aatggcgcag gtagaaggcg cgggtaagcc gctgagtaat ttgcaaaacg ggcaaatggt
    23581 gaaaatccgc cagaacgcca gcggcgtggt gacgggctta accattgata cggggaataa
    23641 tcaacaggtc ctgtttaccc gccagccgga tggcagtttt attcgtgcgc ggtagatgaa
    23701 gggtcaacca aaagccagct aaggattgtc tctttgatga ccttacgtct ggcttccagt
    23761 gcctgggggg aaaccatact gatatgataa accaggccaa gcgtatactg gttgatcaaa
    23821 taatatcccc caagcgcagc aatattaata ttcacctgca atgggtcgat atctggctta
    23881 aaaatattgt gtttcttccc ttcatccaat aaggattcca ttaactgcaa gtgcgcatgg
    23941 ttaatttcta agagacgctg tgattttgca taatgcacac ctttactttg attttcacta
    24001 tgcacaattt ttaaaaacca ggggttagca atataataat cccatgtaaa atcaataatt
    24061 ttctcaatag ctttttgtgg cggaagatct gagaaatcaa gggccttttc tttttgtcga
    24121 atatcgttcc aggtatattc cagtacttca ataaataaat tttctttatt accaaagtga
    24181 tgataaaccg tctgtttact acaccccgca gccttaacaa tattatcgac cctggcccct
    24241 tcataaccat attcggcaaa ttcattaacc gcgcataaca gcagcttttc cttcaggctc
    24301 tgtacgttgc tggtatcaag atacataacg cttccccacg acctcttcaa gataactatc
    24361 ttaacccgat taattagaag ctccagtaaa aaggataaaa acttgtactt tcatcaagtt
    24421 atcaaatgta ctgtgatcac tatcgcatat caaccaacca gatggttgat ctttcttgcg
    24481 gcacgtcgca agctaattgt cacttagaaa acaatattca aaaaaagaga gctattgtat
    24541 gaaatccact cgtcctgttg ccgtgattac cggagccgct cgtggtattg gaaagggctg
    24601 tgcgctggag cttgctcgtg gcggatttaa cctgctgatt aacgatcttc ccgatgccga
    24661 cagcgtagaa aaactgcata tcacacaaca agaatgtata gctgaaggcg tggaagtgat
    24721 ttgcttccct gccgatgtcg gcgatctctc cttgcatgaa gagatgctcg acgcggcgca
    24781 aaaccagtgg ggacgtctgg actgcctgct caacaacgca ggcatttcag tgaaaaaacg
    24841 cggtgacctt ctcgacctcg aaccagacag cttcgaccaa aatattgcca ttaacacccg
    24901 cgcgccgttc ttcctcgctc aggcattcag taaacgtctg ctcgcacagc cgaaaccgga
    24961 agcagaactg ccgcaccgct cgattatttt cgtcagttcc atcaacgcca tcatgctggc
    25021 gatgaatcgc ggcgaatata ccattgccaa aaccgccgtt tctgccgctg ctcgtctgtt
    25081 tgctgctcgc ctttgtaatg aacaaattgg cgtttatgaa gtgcgccccg gtctcatcaa
    25141 aaccgatatg actattccgg caacggctta ttacgacgaa ctgattgcca aaggtctggt
    25201 gccgtgggga cgttggggtt atccggctga tattgcttcc actgtccgcg cgatggcgga
    25261 aggtaaactg atttacacct gtggtcaggc ggtcgccatc gacggcggct taagtatgcc
    25321 gcgcttctga ggctgaatca tgagagatct gttacaacgc ccggatttat tcagtatcaa
    25381 taccgcaaca ctgggctata aaacgccgct acccgccatt attgatgcct gcgccgcgcg
    25441 tggtattggc gcgattgctc cctggcgcag ggagttgcaa ggtgaagatc tccagcaaat
    25501 cgcccaccag cttgccgcca gtaacatgag cgtttccggt ttgtgccgta gcacttacta
    25561 caccgcgccg acactggcgg aacgcaaact ggcgattgat gataaccgcc gggcgctgga
    25621 cgacgcagcg gtgcttaacg ccgcctgtta tatgcaggtg gttggcggtc tgccaacggg
    25681 cacaaaagat ctgtacgaag cgcgggaaca agtaaaacaa gggattcgcc agttgctgcc
    25741 acattcgaaa gatgtcggcg tgccaattgc gctggagcca ctgcacccga tgacagctgc
    25801 cgatcgctct tgcctgtgca cgttacgcca ggcgctggac tggtgtgatg aactcgatcc
    25861 cgatggtgag tttggcctcg gcgtggcggt ggatgtttat cacgtctggt gggatccgga
    25921 tctcgccagt cagatcctgc gtgccggaaa acgcatcctc gcgtttcatg tttccgactg
    25981 gttagtgccg accactgatc tggtcaatga ccgtggtatg ccgggagatg gcgtcatcaa
    26041 tattccgtca attcgtcggc tggttgaaaa cgccggattt aatggtgcta ttgaactgga
    26101 aatattctcg ccttactggt ggcagaaaga tattaacagc acgctggata ttagcgtcga
    26161 tcgcatcgcg cattattgtt aaggaatgat catgagtgac caacccgttc tgttcagcag
    26221 agtggcggct tcctgccgcc tgacattgaa ccgtgaagat aaatgtcatg ctattaacga
    26281 agagatgatc gaatctctcg accattattt aaatgaaatt gaaaacgaca ccacattacg
    26341 attagtcgaa ttaacggcaa ccggcgataa attcttttgt gcgggcggcg atattaaatc
    26401 gtggtcggcc tattcgccat tagatatggg cagaaaatgg attaaacgcg gtaatgacgt
    26461 ttttaatcgc ctgcgaaact taccccaatt aaccgttgcg aatctcaacg gtcataccat
    26521 cggcggtggt attgaactgg ccttgtgctg cgatattcgt attgcccgcc ccggcgcgaa
    26581 attctctaac ccggaagtga tgctcggcat ggtgcctggc tggatgggca ttgaacgtgt
    26641 gcttaatcag gttggtccgg tggtcggtcg ccagatgctg atgctgggta aacgcctgac
    26701 ggcgcaggaa gcccaggcgg cgaatttaat tgatgaagtg gtagaaatag aacaggtgga
    26761 aagctggatg gcgaaccagt tagcgcagct ggaaaaatgc ggtccggtgg cactggcgca
    26821 tattaagcaa ctgattctgg cactggaaaa taaacatgcc gattacccgc accagctact
    26881 ggccggatta atgtccgcca ctcaggattg ccagcaggcg acgcgcgcct ttgccgaaaa
    26941 aagtagcgtg agcttccata atcaataagg aaattgtatg cgtaagataa caaccgccga
    27001 ggcactggcg gcgcaaattc aggacggcgc aaccattgcc attagcggta acggcggcgg
    27061 catggtggaa gccgaccata ttctggctgc tattgaagcg cgtttcctgc aaaccggaca
    27121 ccctcgcgac ctgacgctaa tccactcgct gggtattggc gatcgcgact gcaaaggcac
    27181 taaccgtttt gcgcacgcag agatgctcaa acgcattatt gccgggcact ttacctggtc
    27241 gcccaaaatg caggcgctgg taaaaaataa cacgattgaa gcctactgct ttcctggtgg
    27301 cgtgattcag gcactgctac gggagatcgg cgccggacgt ccggggcttt ttacccacgt
    27361 tgggctggga tcgtttgttg atccacgcaa tggcggcggt aagtcgaatg aatgcactac
    27421 cgacgacctg gtagaactga ttgaaatcga cggtgaaacc aaacttcgtt atcgcccttt
    27481 caaagtggat tacgcgattt tgcgtggcac ttatgccgat cctcgaggca acgtcagcct
    27541 tgaagaagaa gcgattgata tggatagcta ttccatggcg ctggcagcac acaacagcgg
    27601 cggcaaagtg ttcgtacagg tacgcgatgt gctggaagct ggcgcgattg aaccacgccg
    27661 ggtcaaatta ccgggcattc tggttgatgg catcgttgaa caccgcgaac aaccacaaac
    27721 ctatcttggc ggttacgacc tgaccatcag cggccaacat cgccgtctga gttctaacga
    27781 cgccattgaa ctggttagtc atccggtacg tcgcctgatt gcccgtcggg cggcacgcga
    27841 actggtagct ggcgcttcaa ccaactttgg ctttggtatt cctgggggta ttccaggcgt
    27901 agcgctgcgc gaaggcgtgc cttaccaaag tttatggctg agcgtcgagc aaggcgtgca
    27961 taacggtatg atgctggatg atgcgttttt cggctgcgcc cgcaacgccg atgccattat
    28021 tccatcactc gatcaatttg agttctacag tggcggaggg atcgatatca ccttcctcgg
    28081 catgggagag atggatcagt acggtaacgt caacgtatcc catctcaatg gcaatctcat
    28141 tggtcccggc ggttttctcg aaattgcgca aaacgcccgt aaagtggtgt tctgcggcac
    28201 gttcgacgcc aaaggtagca agattgatgt aacgccagat ggcttgcata tcacccagtc
    28261 aggtcaaatc cctaaactgg ttacccaggt ggaaaaaatc acttttagcg ccgcctacgc
    28321 acagcaaagt ggtcaggaag tgttgtatat cactgaacgc gcagtattcc agttaacggc
    28381 agaaggcgtt gaattaattg aaatcgcgcc gggtgtggag attgagcgcg acattctgcc
    28441 gtatatggct ttccgtccaa ttatcaagca cccacgcctg atggaaagta gcctgtttac
    28501 gccgatggag gatgcatgag tcagcttgat gacaccatcc ttgatgcgct gacgcacgtt
    28561 actttcccaa agggttttgc acaggcagag cccgcatggg ttgtcacggt agacggtgtt
    28621 gattacccac tctggcaaac agatgccctg gtagtcggca gtggtgcagc aggattacgt
    28681 gcggcagttg aacttaaacg ccgccagcaa aatgtgctga tcgctaccgc cgggttatat
    28741 atggggacgt cggcatgttc tggttccgat aaacagacac tatttaccgc cgctaccgcg
    28801 ggcaacggcg acaacttcac caaactggca gaggcactgg cgagcggtgg ggcgatggat
    28861 cacgacaccg cttatgtcga agcggtaggt tctctgcaca ctctaggcgg gttgcaatat
    28921 cttggtctgg aattaccgga agatcgctat ggcgcgattc ttcgttatca aaccgaccat
    28981 gacgaagccg ggcgtgcaac ctcgtgtggg ccgcgtacct caaggttaat ggtgaaagtg
    29041 ctgttggaag aagtacagcg cctcgccatt ccagtgttga ccagtgcaac agtgattaaa
    29101 ctgctgcatc agcgtgacga aaacggcgaa gaccgcgtgg caggggcaat cctcgcgacc
    29161 ggtcatcgcg cccataaccc atgggggctg gcaattgtga ctgcgcccaa tgtggtactg
    29221 gcaacaggag ggcctggcga gctttatcgc gacagtgttt atccacataa atgcttcggc
    29281 tcgctggggc tggcgctgga ggaaggtctg acgctaacca atctgaccga aagccagttt
    29341 ggtataggca cgccgcgtag cacgtttccg tggaatttat ccggcaccta tgtacaggtg
    29401 atcccgtata tctattccgt ggatgctgag ggtaacgagt ataacttcct cgcggattac
    29461 tatcgcacca cccaggagct ggcttcaaac attttccgta aaggctatca gtggccgttc
    29521 cacgccacgc gggtgatgga ttttggctcc agcttgttag atatggcagt agcgcaagag
    29581 cagcaatcag ggcgtcaggt atttatggat ttcaatcgca atcctgaagc ggtgccgggt
    29641 gacctgccgt tctcattaga tcgactggac gacgacgttc gcgcgtatct ggaaaataac
    29701 gacgctctgg caccatcgcc catcgaacgg ctgcaacgaa tgaatccgct gtctatctcg
    29761 ctgtataaga tgcacggtta cgatctcacc acacagcctt tgcagtttgc catgaataat
    29821 cagcatatga atggcggcat tgaagtggat atctggggac aaacctccct gcccggctgt
    29881 ttcgccgtgg gggaagttgc tggcacacac ggcgtcactc gccctggtgg tgcagcattg
    29941 aatgccgggc aggtttttgc tgttcgtctg gcacgtttta ttggttgcac gcaaaagcgg
    30001 aatattgatg gagatatggc acagctggta gctcctacac tggcttctat aagagagatc
    30061 attactcagg cgcacgataa cgggaccggg atgccgttgt cggttgtgag agaaaaaatt
    30121 caggcacgaa tgtctgacca tgcgggattt atttgccatg ccgataaagt ccgacgcgcc
    30181 actcgcgatg ccctgctatt gagcgaattt gtccaacggc atggattggc tatcaaacat
    30241 gtgggtgaag ttgccgagct gtttatgtgg cggcatatgg cactgacttc tgccgccgta
    30301 ttaacgcaat taacgcatta tattgacgca ggtggtggta gtcggggagc acgtattatt
    30361 ctcgacaggg atggaaattc tattccgcaa acacgaaatg gtttttgtga tgcctggcgc
    30421 tttcgttcag agcgtactga ggataaaaaa gataaactac ttattcatta ttgcaacggt
    30481 atctttcacg tcagagaaac acctgttcgt gagtttccta ttatccgagg tatttggttt
    30541 gaaaaaaact ggcctggttt tcttaatggg acaatttacc agccacagga tgaatgatat
    30601 tgcaaaggtt aatcactatg cgaaatataa ttagatctat taatgctctg cttgcagcat
    30661 tgaacattac cattttggcg atcatcgtgg catgtgtgac gtggcaggtg gctgcacgat
    30721 ttatatttac tagccccagt atttttacag atgaactcag ccgtttgctc tttatttgtc
    30781 ttggactttt tggtggggca tacaccgcag ggcaaaaccg gcatctggcg attgacctgc
    30841 tacctatgat gctgaaagga aaagcccgac gtcatttatt tttgtgtatt cagattatcg
    30901 ttattatctt cgcaaccatc attatggtat atggtggtgg gctattaacg atggatacat
    30961 tcgattcagg tcagacctcg cccgctttag gctggcaaat gggatatatc tatatgagca
    31021 tcccgattag tggtgtactt attattatat acaccataga tatggtttta accgaactta
    31081 aacaaccact ctaaagataa caaacaacaa ccaaccatag taggaatgga gaaattatgg
    31141 attactggtt accgattatt gtgttatttg gtgccttctt ttttatgttg gcattaggtg
    31201 tacctattgt ttatgccatt ggtttatcga cactggcatc aatttccaca caactcgatt
    31261 tcaactcagc attgagtgtg gtttcccaaa agttagcctc cgggttagat agttttacgt
    31321 tgttggcaat cccatttttt atcttgtctg gcaacattat gaaccatgga ggaatagcgc
    31381 gacgattaat caactttgcc cgtattctcg gcggtcgtct gccaggctca cttgcccact
    31441 gtaatattct cgccaatatg ctgttcgggg caatttctgg ttcagccgtg gcttcggcgg
    31501 ctgcgatggg cggcgtcatg catccgcaac aggttaaaga gggttacgac ccggcattca
    31561 gtactgcggt taacgttgcc tctgccccga cgggtttgct tattccacca agtaatacgt
    31621 taatcgtcta ttcactggtc agtggtggta cttctattgc cgcactgttc ctggcgggtt
    31681 atgttccggg aattttgctg ggtctggcac tgatggtaat agccggtatt atcgcggtac
    31741 gtcgcggtta tccaaagcca gaacgcccga ctttacgcca ggctggcgtt gcgatatgga
    31801 tggctatccc cagtattttc ctgattattt taattatggg gggtgtatta agtggtattt
    31861 ttaccccgac agaggcctcc gcaattgcag taatttatac ccttttcctt gcactggttc
    31921 tttatcgtga aatctcagtt aaagatttac ctaaaatatt tctggaatcc gttattacca
    31981 ctgcaattgt cttgttatta attggttctt cgatgggtat gtcatgggca atgtcaaacg
    32041 ctgacgttcc cttcctgatt ctcgatttgc ttaacactat ttctgacaac ccaataataa
    32101 tcctgcttat tatcaatatt atcctgttga ttatcggcac ttttatggat atgacaccgg
    32161 cggtactgat ttttacgccg atatttttac cggttgtcac cgaactgggc atggacccga
    32221 ttcactttgg tattgtcatg gtactgaaca tgtgtatcgg tatttgtact ccgcctgtag
    32281 gcagtgtgtt atttgtcggt tgtagtgtat ctaaattacc gattaataaa attatcaaac
    32341 ctatgctgcc attctatgcc gtgatggtat tggtgctggc aatggtgacc tatattccgc
    32401 aaatcagtat ggcattgcca agagcactgg gctactgatg cgcattcact ggcggcacct
    32461 gccgccatat ttattagaaa aggaacatct atgaaaataa aagtatccgc tggcattatc
    32521 ggtgctgttc ttatgttatc cgcaagccag tcctgggcag tgacattaaa actgagtcat
    32581 aatcaggata agtctcatcc tgttcataaa gcgatggagt tctttgcgaa aaagagcaaa
    32641 gagtactcta acggtgatat tactattcgt atttatccaa atggaacatt gggtactcaa
    32701 cgagaaacaa tggagctgat tcgttctggc gctattcccc tggtaaaaac caatgcggca
    32761 gaaatggaag catttgaaaa ttcctataaa ttatttagcc tgccttattt gttccgcgat
    32821 cgtgatcatt attatcaggt catgcagggc gatatcggga gaaaaatcct cgactcaacg
    32881 aaaagcaaag gttatttcgg gctgactttt tatgatggag gcgcccgcag tttctatggc
    32941 aataaaccag tactgaaacc agacgatctg aaaggcatga aagtccgtgt ccagccaagc
    33001 cctggcgcag ttgaaatgat caaagtcatg ggcggtaacc cgacgccact ggattacggc
    33061 gagttgtata cagccttaca gcagggtgtg gtcgatatgg cagaaaacag cgtgatggcg
    33121 ctgactacca tgcgtcacgg tgaagtggca aaatccttca gccttgacga acacactatg
    33181 gttcccgatg tggttctgat gagcaatgct gcgtttgata aacttagccc ggaaaatcag
    33241 gcagttatat taaaagcagc taaagaatca atgagctaca tgaaagactt gtggagcgag
    33301 gaagagaaac aagaatttgc aaaactggat aaaatgggcg tgaaagtcta ccaggtagat
    33361 aaagctccgt ttatcgagaa agtacagcca atgtacgcaa acttcgctaa ggacaaccca
    33421 gcccttgccc caatgctggc tgatattcag gcagctaagt aattatcttc gtagggctgc
    33481 ttattgtggc ccttatcgat atctgcaagt gctcgctaca aaagcaaaac gccgaccaat
    33541 ggtcggcgtt tttacgtctc gttgaataac gaattattca gctactacgt ttacgatcac
    33601 tttcgcgaat acttcgctgt gaacctggaa gctcacttcg tgttcgccag tggtacgcag
    33661 aacgccgttc ggcagacgaa cttcgctctt agccacttca acgccagctg cagttacagc
    33721 gtcagcgatg tcgcgagtac cgatggaacc gaacagttta ccttcgtcgc cagctttaga
    33781 cgcgatggta acagtttcca gtgcattgat tttctcagcg cgagcattag cagctgccag
    33841 aacttcagcc agtttagctt ccagttcagc gcgacgtgct tcgaagaatt caatgttttt
    33901 cttggtagct ggaacagctt taccctgcgg taccaggaag ttacgagcat agcccgcttt
    33961 aacgtttacc tgatcaccca ggctacccag gtttgctact ttatcaagca gaataacttg
    34021 cattacctta tcctctcaaa gtcgtattaa tggaccgtga ccgattactg atggcgatca
    34081 gtgtacggca gcagggacag gtagcgagcg cgtttgatag cgcgagccag ctgacgctgg
    34141 tattttgcac gggtaccggt gatacggctt gggacaatct taccgctttc ggtgatgtag
    34201 tttttcagcg tagcgatatc tttatagtcg atctcttgaa cgccttccgc ggtgaaacgg
    34261 cagaacttgc gacgacggaa ataacgtgcc atatggctag tctccagaat ctatcaattc
    34321 aatctgctcg gcatgcaaaa ccattttgct cagtccgttc tttgccttgt ggcatgaaat
    34381 gaacccctga acggttatgc gactgccgac cgttatactg tgagtaatga cctggttttc
    34441 gtgtccgcta acaataacgg gcatttgaca ccatgcctgc cggtgaaagc cggcttcctc
    34501 ctgcacagaa cgatgctcaa gcacgaactg gcagtgagga attcctgatg gactgacctt
    34561 tcgaagggga gtcctgcaca cggtgccgga caacaccaga cggttggtca tcagaaatta
    34621 ctcttcagaa tccccagctt cagcatcatc agcggtttcg tttgcgaaat catcgcgacg
    34681 ctcacggcgc tcgtctttcg ctttaaccat cggagatgct tcggtaacag cgtgcttggt
    34741 acgcataacc atgctgcgga taacggcatc gttgaagcgg aaggtagttt ccagctcatc
    34801 gatcacttcc tgcggagctt caacgttcat cagaacgtag tgtgctttgt gcagtttgtt
    34861 gatcgggtaa gccagctgac ggcggcccca gtcttccaga cggtggatct tgccttctgc
    34921 accagtgatg gcagcagtgt agcgctcgat catgcccgga acctgttcgc tctggtcagg
    34981 atggaccata aaaacgattt cgtaatgacg catcgaattg ctccttacgg attattcagc
    35041 ctcctgtctg ggtcagccga atcccgggga ggcaaggaac gtgttaaagg tcggctgaaa
    35101 aatgacgcgt tatagtactt gtatgccccg ctaaactcaa ggccagccga caaataaatc
    35161 tcatctttcg ctcaaaagaa ctaactccct gatttgaagt tcatcacatt ccgtcacagt
    35221 gttttttcga acaactgcat cagaaatggt caccatcaac ttctttatca ttgattaaaa
    35281 ttaaatcaga agatcatcac agaggagagc acacaaagtc agccgtcgag gaggtatcta
    35341 tgttcagtcg tgttttagcc cttctggctg tgcttttgct aagtgcaaat acatgggcag
    35401 ccattgaaat caataaccac caggccagaa atatggacga tgtgcaaagc ttaggcgtga
    35461 tttatatcaa tcataatttc gccactgaaa gtgaagcacg tcaggcatta aatgaagaaa
    35521 cagatgcgca gggcgcaacg tactaccacg taattctgat gcgggaaccg gggagtaacg
    35581 gcaatatgca cgccagcgcg gatatttatc gctagcacca ggtataacca acgaaacatt
    35641 gccatagttt gctttgcccc cttcgcaggg ggcttttttt gaaaaacggc tgcggaatta
    35701 gaccagttat ctcccgaaga aggaaatttc cgcagcgcgt gttttacttc tgcccgtaat
    35761 aagcgttagg accatgctta cgcatgaagt gtttattcat caggaagctg tcgatgtgat
    35821 tgagttgtgg attaatactg cgcgcaatcc acgccatttt cgccacttct tccatcacta
    35881 ccgcgttatg caccgcgtcg tgggcatctt tcccccaggc gaacggtccg tgctgataca
    35941 ccacaattcc cggcgtatgc agcggctcgg cgttgcccag cgtttcgata atcactttgc
    36001 cagtgttcag ttcatactca ccctgcacct cttcttcgct taatccacgc gtacacggga
    36061 tgtcaccaaa gaagtagtcg gcgtgcgtgg tgcctaacgc cgggatcgcc agccccgcct
    36121 gcgcccatgc ggtggcatga gtggaatggg tatggacaat gccgccgagc gacgggtaac
    36181 gacggtagag ttcaagatgc gtcgcggtgt cagaagacgg gcgatacttc ccttctacca
    36241 ccttgccgct catatcgacc accaccatat cgtctgcttt catggtttcg taggcaacgc
    36301 cgctgggctt gatcaccacc agcccgcgtt cgcggtcgat agcgctgacg ttgccccagg
    36361 taaaggtcac cagcccatag cgcggcagat ccatgttggc ttcaaatacc tgctgtttta
    36421 gcttttgcat tatgccgcct ccaccatgcc cgctttcgcc atgcgcgctt tcacccaatc
    36481 acgcgctttc gccacttctg ccgccgggtc ttccgccgtt tcgctccaca tctcaatcag
    36541 gtacggcccg caatagccac tctgtttgag cgtttcgaaa caacgttcga aatccactac
    36601 accttcgcca aacggcacgt ttttgaagac gccaggtttg gtgtctttca catgtaccgc
    36661 gacgatatgc ccgattccgg cctgcaactc catctgcaca tcgttgtccc acgccgacag
    36721 gttgccgata tccgggtaga gctggaacca cgggttgttg agatagtgcg cgtaacccag
    36781 cgccttgctg atggagttca tcaacggata atccatgatc tccatcgcca gcgtcacctg
    36841 cgcgcggctc gccatctcaa cgctctcttt caggccgtca cggaaacgac gacgcgtttc
    36901 gttattggct tcctgatagt aaacgtcata gcccgccagc tggatcacgc gaatgccgac
    36961 gtcctgggcg aactggatag ctttacgcat aatctccagc ccctgcgccc gcaccgcgtc
    37021 atcttcactg cccagcggga aacgacgatg agcagaaagg cacatggacg gcacgcgcac
    37081 gccggtttca acaatcgcat tcaccagcgc cagacgctgc tcgcggctcc agtcgaggcg
    37141 cgacaggcgc tcgtccgttt catctaccga catctcgaca aaatcgaagc ccaacgtttt
    37201 tgccagttgc aggcgttcca gccagcactc cccggcgggg agcgcttttt catagatgcc
    37261 aagcgggatt tgtttggaca acatatccgc tccttagccc cacagttcgg cgatggaacg
    37321 tttgaactga cgtgcagctt caaccggaga agcggcatca cggatgctgc gaccagcgat
    37381 aaacacatga atcggaatac ctttgaacag cggcagatct tccagcgcca ggccaccggt
    37441 gacggtgact ttgaagccca tatcggaaag acgtttgatc gcggtaatgt ccgcttcgcc
    37501 ccacgctacg cctgcggcct gcgcgtcacg gctgcggtga taaaccacct gctgaatgcc
    37561 tgcgtcacgc cactgctgcg cctgttccca ggtccagtag ccggtcaatt cgatctgcac
    37621 gtcgccgtta aactcttttg ctacgtccag cgcgcctttg gcggtgttga tatccgcaca
    37681 gcaaattacc gtcacccagt cagcgttggc ttcgaagcac atacgcgaaa ggattttgcc
    37741 cgcatcagca attttagcgt ctgccagtac gattttgtgc gggtagagcg ctttcaggtc
    37801 acgaaccgca cgcacgcctt cgcccacgca cagaatggta cccacttcga taatgtcgac
    37861 ttcttcggca atcaggcgag tggtttcgta ggcgctatcc atagtctggt tgtccagcgc
    37921 gacttgcaac atcggtaatg acatcttcaa ttccttctta agctgccgcg ttggtgcggt
    37981 caatgagatc cagtacttcc tgctcggtac ggcaggcacg taaacggtcg aaattttctt
    38041 catcttcaaa caggttgacg atctgcatga tgcccacttc ctggtgagtg ttggcatcga
    38101 ccgccgccat ggtgatgagt atatccaccg ggtcattatc ttcgtggttg aactccagcg
    38161 gctttttcag cgtcaccagc gcaaaaccgg ttttcttaac accctcttcc gggcgaccgt
    38221 gcggcattgc caggcctggt gcgagcacaa agtacggacc gtgctgttca accgcatcca
    38281 gaatcgcctg gtagtaacgc ggctcgacaa catccgccgc caccagcagg tcaacgccga
    38341 tttttactgc gtcctgccat gtctcagctt cagcctgcag gcggatggat ttattctccg
    38401 ccagcgaatc acgtaatttc atggtgcgtc cttacttcac atcctgcggg aaatgctctt
    38461 tgatcacttc cagcagtttc gggccaaagt cagcaggaga gagcatgttg cgcacgccaa
    38521 ccacgtattt gttgccggtc acggtgattt cgcccgcaat gtgcgtagaa gcgatgatga
    38581 tatccgcgcc gctcaactcg cttttgtatt cgccaaccgc gcagctgttt accgtgtggt
    38641 caatgtttga ttgggttaaa aactggtcca ctttcatctt catgatcatg gaactgcctt
    38701 gtccgttgcc acacacagcc agaatacgta cggtcataat caaaactcct tattaagcag
    38761 actgttctgc cagttgtttt tctgcatctt cttctgcgcg cagtgcgcgg ccagcgaaga
    38821 acatataagc cagtgcaatt acaatgatga cggccataaa ggcgataccg atggagaaga
    38881 agccctgcat catcggcggt gccagaatcg accagtccgc catacccatc caggcactca
    38941 taccggtaag tttcaccgcc cagacgcaac caaagatttc gatcatcccc atcaccagac
    39001 aaatcttcag cgccgcacgc cagccgccga agtggttagc gaacacgccg atggtggcgt
    39061 tggagaagaa catcgggata aagccaggaa taatcaggat tgaggaaccg caggcgacca
    39121 ggatgccaac cgcaatcagc tgaccgatgg tgccccacat aaacccccag accacggcgt
    39181 tcggcgcgaa gctatagata gccgcacagt caatcgccag aaccgcacct gggatcaggc
    39241 gctgggaaat gccgttaaat gcttcagaga gttccgccac aaacatgcgc acaccctgag
    39301 tgatgatgaa gatcgccacc gcgaaggaga aaccagtttg caggatgtac accgtccagt
    39361 tcactttgcc tgccatcgcc tgcacggtgt cgataccgaa ggagagcaga atggcaccaa
    39421 agaagatggt catcacaatc gccgtggaga cgatgttgtc gtggaaaata ttcagccagc
    39481 ctggcagttt gaggtcttca acgctttcct cttttttgcc gaggaacggc gcaactttat
    39541 aggcaatcca tgatgcaaac tgttgctggt gaccgatgga gaaaccgcag ccgtccgtca
    39601 cttcctgagt cggcttgtac attatgttgg aggtaatgcc ccagtagagc gaaaccagaa
    39661 tcgccgtgca gataatggtc gtccacatgg agtagccgaa aataaacaac gtaacggcaa
    39721 tcagccccgc ctgctggaac atgatatgac cagtcagcat gatggtacga ataccggtaa
    39781 tgcgacgcag cagcacgtaa cagatgttca acgccagcgc taacaacacc gcgtaaccta
    39841 cccagctata ggcatcgccc atgcggtcga tggttgccat catcgacgcg taagtatcgg
    39901 aaattgcgcc gttaatgccg tagacttcgg acattttcgc caccaccggt ttgaaggtgc
    39961 tggtgaggat gccggaccct gcctgcaaca acatgaaacc aattatggtt ttaatcgtgc
    40021 ctttaataat aacgctgaca cttttgcgca gtaggatgta gcccagacag gtcacaatac
    40081 ccagcaacaa cggggcattg gtcatgacct ggttaaaaaa cacggtaaag atgttgtaga
    40141 ggatctccat aacgatctcc tgaagaagag gtaaccgggt attccacaca cccggaatgt
    40201 tgtgcactca ctctaatttt caaaagtaat cacaacaaga ttatttatga ttaaatgtga
    40261 cgcgacccgc aaataattcc acatggatag aatgggattt acacaacgaa acaactattc
    40321 atttaaattc atttagttaa ttgaattaca cattccatta aatctttcca cactcaaaat
    40381 caacaaccac ttccctggca aaaagtcaaa aaatggtcat tgccttcctg aaaaaatagt
    40441 gtcaggatta atcaaaacca atcacatatt gattcggatt gaactatcat gaaggtaaat
    40501 ggcgatgagt aaagtgaaaa gtatcacccg agaatcctgg atcctgagca ctttcccgga
    40561 gtggggtagc tggctgaatg aagaaattga acaagagcag gtcgcccctg gcacatttgc
    40621 gatgtggtgg ctaggctgca ccgggatctg gttgaaatca gaaggtggca ccaacgtttg
    40681 cgttgatttc tggtgcggca ctggcaaaca aagtcacggt aacccgttaa tgaaacaggg
    40741 tcaccagatg cagcgcatgg ctggcgtgaa aaaactgcag ccaaacctgc gcaccacccc
    40801 gtttgttctt gatccgtttg cgattcgcca gatcgacgcg gtactggcga ctcacgatca
    40861 caacgatcat atcgacgtta acgttgctgc tgccgtgatg cagaactgtg ctgatgacgt
    40921 accgttcatt ggaccgaaaa cctgtgtgga tttgtggatt ggctggggcg taccgaaaga
    40981 gcgttgcatc gtggtcaaac cgggcgatgt agtaaaagtg aaagacattg aaattcatgc
    41041 gcttgacgct ttcgaccgta ctgcactgat caccctgcct gccgatcaaa aagcggctgg
    41101 cgtactgcca gatggcatgg acgatcgcgc agtaaactac ctgttcaaaa cccctggcgg
    41161 ttccctatat cacagtggcg attcccacta ctctaactat tatgcgaagc acggtaacga
    41221 acatcagatc gatgtggcgt taggatcgta tggtgagaac ccgcgcggta ttaccgacaa
    41281 aatgaccagc gccgatatgc tgcgtatggg tgaagccctg aatgcgaaag tggttatccc
    41341 gttccaccac gacatctggt caaacttcca ggccgatccg caagagatcc gcgtgctgtg
    41401 ggagatgaaa aaagatcgtc tgaagtatgg cttcaagccg tttatctggc aggttggcgg
    41461 caaatttacc tggccgctgg ataaagacaa cttcgagtac cactatccgc gcggtttcga
    41521 tgattgcttc actattgaac cggatctgcc gttcaagtca ttcctgtaat ctgtcgttta
    41581 taccggatag cgtggagaag ttcgcgctat ccggcgtagt atttcctggc tatttcaaat
    41641 atcatctaaa aaaatcaaat tttatcggaa tagctcatga ctgaagcaca aagacatcaa
    41701 atcctcctgg aaatgctcgc acaattgggc tttgtgaccg ttgagaaagt cgttgagcgt
    41761 ctgggaattt cgcctgccac tgcgcgacgc gatatcaata aacttgacga aagcggcaaa
    41821 ctgaaaaaag tgcgcaatgg cgcagaagct attacccaac agcgcccgcg ctggacgccg
    41881 atgaatctgc atcaggcgca gaatcacgat gaaaaagtac gtatcgctaa agcggcctcg
    41941 cagctggtta atccgggcga aagcgtagtc atcaactgcg gctccaccgc gtttctgctt
    42001 gggcgggaaa tgtgtggcaa gccagtgcaa atcatcacta attatctacc gctggcaaat
    42061 tacctgatcg atcaagaaca tgacagcgtg atcattatgg gcggacagta caacaaaagt
    42121 cagtccatca ctttaagccc gcagggcagc gaaaacagtc tctatgccgg gcactggatg
    42181 tttaccagcg gcaaaggact gacagcagaa gggttgtata aaaccgatat gctgacggca
    42241 atggcagagc agaagatgct gagcgtggta gggaaactgg tggtactggt tgatagcagt
    42301 aagattggcg aacgcgcggg aatgcttttt agccgtgccg atcaaattga tatgctgatc
    42361 accggcaaaa atgctaaccc ggaaatcctg caacaactgg aagcgcaagg ggtcagcatt
    42421 ctgcgtgttt aaagatgctg gcggaaaaat gtcactgcag catctaacgc ctcaggcgta
    42481 atgcggtgac gcacgcctgg ctgccatgaa caggttagca gtttatcccg tcccgtctcg
    42541 cttaaggcct gctgcaaacg tagtgattcg tcggcaggca caacgtcatc gtcgaggcca
    42601 tgccacagca gtagaggtct gtcactaagt tgttccaggt ggtttgtcgc ttcccactct
    42661 gccagcggcg cgacaatgtt attgaattca ttctgctgtg ctgtcgtttc aggtatcagc
    42721 ggtggaaaca gtgaacgggc gagtgatgta aaatagcccg ctcccatcat gctggcggta
    42781 catctcaccg tggggtggcg agcggtaatc cccagtgccg tcatcgcgcc catcgacgca
    42841 ccaccgactg ccagacggtc atcaagcagc cagttttctt cggctattgc cgcacgtaaa
    42901 gtagtgaatt cctgcatact ttgtagcaag atttgccaga attgatttaa ccgccgcgct
    42961 gcgtcaccac taaaacggct accgtgatcg ggcgcatccg gcatgatcac ccgcaaacca
    43021 gcctgcgcca gcgcaacggc aaaatagcta tacaccagac tggatgaagt aaagccgtga
    43081 taaaaaatta cgcacggtaa cggggtatct ttttgcccga caggataagc atgaagaacg
    43141 ggaatatctg ccagctcgcg tgattctatt tcaatcatct gtctctcctt tttgtctggt
    43201 atgacatgaa gaaatcctta gcacaagttt ctgctggcgg tattagttca taatgttgag
    43261 atgtgggtta cgctttcgtc acgttttcat tcgaaaatcg cgtaccaggt aacaattcgg
    43321 gaacattccc ccaaagttaa attaataagg cactacacta tgtttagcag gaaacgtaat
    43381 agcgttattt accggtttgc cagtttatta ttggtgttga tgttaagtgc ctgtagcgca
    43441 ctgcaaggta cgccacagcc agcaccgcca gtaacggacc atccgcaaga gattcgccgc
    43501 gaccagacgc aagggttaca gcgaataggc agcgtaagca cgatggttcg gggttctccg
    43561 gatgacgcat tagcagaaat cagagcgaaa gctgtcgctg caaaagctga ttattacgtt
    43621 gtcgtcatgg tggacgaaac catcgtgaca ggacagtggt attcacaagc cattttgtac
    43681 cgtaaataaa tcagacaact tacatagtca gctttacatt gctttgcgtc cctatgcgtt
    43741 tacctgtgca caataaatta ccggctggaa gcctgggcgt ttattaccgc gatggaatgc
    43801 cctgcaacat gtggggaaac gaaaaatgga gctgacgatg aaacaattac ttgcctcacc
    43861 ctcgctgcaa ttagtgactt atcctgcgag cgccacggca cagtctgcag aattcgccag
    43921 tgctgactgc gtgacgggcc tgaatgaaat aggtcagatc tcagttagca atatttcagg
    43981 ggatccgcag gacgtggaac gtatcgtggc gttaaaggcc gatgaacaag gtgcgtcatg
    44041 gtaccgcatt attaccatgt acgaagacca gcagcctgat aactggcgcg tacaggcgat
    44101 actgtatgca tgagtgtcat ctacagcaaa tgaacaatgc cggatgcggc gcgagcgcct
    44161 tgtccggcct acaattttca catatttcat ttagttaatc gaaaccagcg tcgcatcagt
    44221 cgtatacgct tacacacaca ctccccccgt cgcccgcagc aataaatcat tctggatctg
    44281 ctctgacaac cgtacgccgc cgcgcgtatc taacatcacc tgacaccacg cctgcgccat
    44341 tggcggagaa gcatatttca acatttgcgc accgcagccc agcaggaata gctgatgagt
    44401 aatctctcgc ctcagttctt cagctggctt acgcagctgc tgctgtaaac gacgaaccgc
    44461 gcgatcaaaa tagcgatcct gccctttcac ttccacaaac gcttccgaca ataagtcgta
    44521 tacgcccgct tgcttattga gaacgcgcag cacatccagg cacataatat tgccggaacc
    44581 ttcccaaata ctgtttaccg gcatctcccg gtaaagccgc ggcagctcgc tctcctcgca
    44641 ataaccaatg ccgcccagca cctccatcgc ttcggccaca aacggcatac ctcttttgca
    44701 gatcacaaat tttgccgcag gcgtaaataa acgcgcccac agggcttctt tggcatcggc
    44761 acgccggtcc cacgctcgcg caagacgaaa cagcaacgcc gtttgccctt caagctgaag
    44821 tgccatgcga cttaagacat gacgcataag gggctgttgg atcaatggat taccaaaaac
    44881 atggcgttga tgtgcatgat aaatcgccag cgaaaatgca cggcgcatca tggcatggct
    44941 acccagggcg caatcaaaac gcgtcatccc acccattttc aggatcagac gaattccttc
    45001 cccttcctgc cccaacaacc agccaatggc atcctgaaac tccacttcgc agctggcgtt
    45061 agagcgatta cccagcttat ctttcagccg ctcgaggcga atcgcgttgc gttgcccgtc
    45121 aggcaaaaag cgcggcacaa aaaagcagga caaaccgccc gtagtctgcg ccagcaccag
    45181 atgcgcatcg ctttgcggca ccgagaaaaa ccatttatgc cccaccagcc gataagagcc
    45241 atcttccagg cgctctgcgc gggtggtgtt gctcatgaca tcggaaccgc cctgcttttc
    45301 cgtcattccc atgccaatca acaaaccgcg tttttgccca cctggcaata agtgagaatc
    45361 gtagcgatcg ctcagcagcg gcgtggtcca gtcctgaaac ggcgcgggta acatctgtaa
    45421 cagcaatggc gtggcggcaa aggtcatggt tatcggacat aacgaccctg cctcaacctg
    45481 tgcgtgcaac ataaaacgcg ccgcgcgcgc cacaaatgcg ccggagcgag cgtcttcttc
    45541 ccaggcaaga ttgtgcaccc gattggtaca tagcgcctgc atcagcaggt gccaggcggg
    45601 gtgaaaacgc acatcgtcca gacggcgtcc ttgcgcatcg tagcgcaata attcaggcgg
    45661 attcacattc gccagccgcc ccagttcaag ggattcagcc gttcctaact gctgaccaat
    45721 actggctaga aaatcgctat cccagccagc accttcacgc gttaccgctt cgcagagcgc
    45781 gccatcagac aggtataagt tgctgttatt taatggtata ggttgattaa aaacggtgtg
    45841 agtttgccag tgcactgtgt ctccctccat caatggcagt caccattagt atggtcactg
    45901 ccatggattc atgacagaaa gagattgcta aaacattctt atccagcaat gtaatgcgga
    45961 acgaaacgtg aggtgtcttt ggtgatcagt gtgttatctt cacggatccc cattccgcac
    46021 gcttcatcat cgacaatcca gctaccgatg agtgtgtagc tatcgccaaa tcgcggcaga
    46081 ggttgaaacg cctggtagat catcggttca tcggcgtaat caccatcagc gtggtcaacg
    46141 acgttattct gaccgtcgaa aatggtgacg tttccgcctt cgcgcgagta gatcggttta
    46201 cgcacatagc tttcgccagc ggcaatctgc gatttttcac cctcaaacca ggatgcaaga
    46261 aggttaggat gaccagggaa gaagcgccaa agcagcggca ttagcccttt attactcaag
    46321 atacttttcc ataacggctc cacccattgc tcgcgacgct tgcacagcag cggaccgtta
    46381 tcgtcacgca tcatccactc cagcggatac agcttaaatg cacgctggat gacattatca
    46441 tcaagatcgg tcagtacacc gccgacgccc aaaccgagat cttcaatgta gataaaccgc
    46501 gactcctgcc ctgcctgctg ggcgcagtcc tgcaagtaca gcacggtagt cctgtcttca
    46561 tcggtgtcct gacagcagca gaaataaaac ggctcccggc tataaagctc gctaaagcgt
    46621 gaaatcaggc gttcctgaat ggcattgtac tgatcggcat cacgcggaat aatgccgctg
    46681 cgccgggcat cctccagcca caaccactgg aaataagccg actcatacaa cgaggtcggc
    46741 gtatcggcgt tgtactccag cagcttcacc ggcgcattgc cacaccaggc aaaatccatc
    46801 cggccataca gcgaaggatc gcgtgcgcgc cagctttcgg cgatcacatc ccagtacaac
    46861 ggcgggatcg ccagttgcgt caggatctct tcgtctttaa ccgcgcgatc caccacctca
    46921 aggcacatct gatgcagttc cgcagtcggt ttttcgatct gctcttcaat ctggcgcaga
    46981 gtaaaacggt aagcccgact ctcgtcccaa tagatttcat tgtcgatgat atgaaagtcg
    47041 aaaccgttgt tagcggcgat ctggtccaga tcccgtcgca caggaacgtt gtgtctcagc
    47101 atgattagcc tccccaatgc ccacgggcgc tggaagaacg accatagccg ccgcgcgaaa
    47161 cggttgacgc ttttttggtg gtaaagccgc ctgaagagta agactctttt ttgccagaac
    47221 cggagcgcca ggagtaatca ccggaagtgt tgcgccagac ggggcgggaa gcaaatgagg
    47281 atccgccgct gttataaaca aactgctcat cgcgatcttt acgaataacc cggcttaaca
    47341 aaaatccaga tattaccggg atccaactct gttcaacgtt gtcgtagtaa caattttcat
    47401 acttagactg acagttctgc tgagtcaaat tcttcggaac atcggcataa aacgccgctt
    47461 tggcgttatt ccagccacgc gcgcaaatgt cggcattatt accgtcatca atacaatcct
    47521 gcactgtcga gtaaaacgtt ccgtcgccgt cgttatcgac atcgctactt tcactccatc
    47581 ctttgactat taaaaaaacg gcaccgccca ttagcaccaa cgttaagtat ttttgtgcgt
    47641 agcgattgcg gcgcggctca aaaggattat gcggtcttcc aatgcgactg atcgctccgt
    47701 gaccaatttt actgttattt cttgatttgc gttttctggc catgatcgtc cattaccatg
    47761 tcatacaagc tgcgttaaaa ataccgccag ccagcgcggc ggttcccata aacattcctg
    47821 ctgcggtatt gtgattaata attttttcgc ttaatgcggg catataaagt ctgacaccag
    47881 caaaaacgag taactgaatc accagtgcaa tcccgcccca ggcgaaatag tctggaatac
    47941 tcaccgcatt gattgccgca ctggataatg ggataacgta acctaacaat gtaccactga
    48001 acgccagtga agccgcggta ttattgtttt tgattaactg ccattcgttg tgcggtgtaa
    48061 ttttagaata gataaaaaga aaaataatca ccatagccac gccaataaaa aaataggcgc
    48121 taaaggcaag aagtgaatcc agtatatgca ttgtaaacgt ccttgtaatt aaccaataat
    48181 atgaagtgat gtcagcggga tatcgacacc caacgcgcgc gaaaataacc actctggctc
    48241 gccgagatcg ttaaaagatt cctcaccatt taacagcaaa tattcgtaag tatcttcggt
    48301 tacttggcgt tggtagccca tggtaaaatt atggacctcc catttggcat ggttttgatt
    48361 ttctactttt tccagcatgt agacgggttc gatatttccc ggttcttcgc tgttaaaaaa
    48421 tcgctgccag cgtttttcct gccagtttaa gatcattgcc cccatcactt tggcgttgat
    48481 cgcctcgcgc cagtgactct ctttactgat accgtagctc tcttcataga caaagagctt
    48541 gatatcatca atgtcatcaa tatcttcgcc gccggtggta ttgatttgca gaaattcatc
    48601 gcctgaagtg tagtagcgga aaatctgact accgccgccc agatcgatgt ggctgacagc
    48661 ggctaccgta aattcctcac ccggcagcgc aatcagcaat tcatcttcca gcaaacgaaa
    48721 cgctaacgtg tcgagcgtaa agccactatt gagatgaagt cccagcggac cgcgagcgat
    48781 agcaggctta ttatccttgc caaacagacg ctggaaaaaa ccagacatat tttatcctta
    48841 aaatagttgc cagcctttta gcgaggctgg caaaaaaatt actcgccctg ttggcgttgc
    48901 agtcttgcta atacatcctg ggcgctactt ttattgctac cgccaattcc ggcttccgcc
    48961 agcttttcat caagatcgcg accgtctgcg actttttcca actgtgcagc agcatccagg
    49021 cgagcctgac gttcggcctg acgcgtttgc aggcgtttta aggattctgc cgctgtcgaa
    49081 acgctggaag atgcgccaac ggtagaggtt gttacagcct gttgtgcacg ctgcatggct
    49141 tcagtggctt taacgacttc catttgctgc tcaaactgag cgatacgctg cgctgtcgct
    49201 gtaactgctt tttccacgcc atcacgggag acttccagat tcgacaacac ttgctcttca
    49261 gcggtaatga gattctcaag gcgcgcgatt tcttcagcaa cttcgttaat caacgacgga
    49321 ttaacgttct tgcttaacgc ttccagcgca cgggcttcca gactggcttt gcgctcacgt
    49381 aaatctttca gcttatcgtg acttaatttc acccgcgcca gcagatcaac gcgagatttt
    49441 ccggctttat cgagttcagc tttagcgtca cgaatatgct gttccagcat gcgcacgccc
    49501 tgggtttctt caatggattc ttccgcctgg gagataaacg atttccccag cgtaaataaa
    49561 ctttttaaaa ttcccataaa tacccgtcct tgtaattagt gtgaatattc ttctgtaatt
    49621 tcagccagat ccaatgcgtt atctaccagc gaggttatct caagcaggat atcttcaaga
    49681 gaagatttaa gcgataacgc cccgaaaaca atgtaatact cttcctgttg cacgctggag
    49741 attcctaccg atgacaacgg catcatcttc tgatttctta ataagaaggt attaaattca
    49801 tctggattgc tgatactact taccggacaa ataaaggttt cgataatcat ttggcgggaa
    49861 gtaaaaagaa tattgatttg caaatcgcca taatcattca ttttaataat taatgcgctc
    49921 tcgctatttg tcacatcaat attttgttca ggtacagttt gcagcgccgt cgccagcgcc
    49981 aacggattcc atgtcatata ttattccata tagatttagt ttaaatatta ataaaatgaa
    50041 tatttgcagt acgtaattat cttaccagct atagacaaaa aaaaaccatc caaatctgga
    50101 tggcttttca taattcagac gaactagctg cgctgacgaa ccgcttcaaa taagcaaatt
    50161 ccggttgcaa ccgaaacgtt cagggaagaa acacttcctg ccattgggat gctgatcaac
    50221 tcatcgcaat gttcacgggt caggcgacgc ataccttcac cttccgcgcc catcaccagc
    50281 gccaggcgtc cggtcatttt gctctgatag agtgtatgat ccgcctcgcc tgccgtaccg
    50341 acgatccaga tattctcttc ctgcaacata cgcatggtgc gcgcaaggtt agtcacccga
    50401 atcagtggaa cgctttctgc cgcgccgcag gctacttttt tcgccgtagc gttgagctgt
    50461 gcggagcgat ctttcggcac aatcaccgcg tgaacgccag cagcgtccgc gctacgcagg
    50521 cacgcgccga ggttgtgcgg atcggttaca ccgtcgagga tcagcaggaa cggttgatcg
    50581 agcgaagcga tcagatccgg cagatcgttt tcctgatact gacgtcctgg cttcacgcgg
    50641 gcgataatgc cctgatgcac ggcaccgtcg cttttctcgt cgagatattg gcggtttgcc
    50701 aactggataa ccacgccctg agactcaagg gcgtgaatca gcggtaacag acgtttatct
    50761 tcacggcctt ttaaaataaa gacttcctga aaacgttcag gggcgcgctc cagcagggcc
    50821 tgcactgcgt ggatgccgta aatcatttcg ctcattaatg tactcgttgt ttacgttttg
    50881 cctgatgcac tacgtttatc cggcctacat gatctctgca atatattgaa tttgcgtgat
    50941 tatgtaggcc ggataaggcg ttcacgccgc atccggcatg aacaaagcgc attttgtcag
    51001 caatctaacc ctcttctttt aaagagggta ttgatcactc tgccactttt ttcttcgccg
    51061 cacgcttcgc tttggtcgct gcggctattt tctgcgtttt cgccgatggc tttttcgctt
    51121 ttctcgcgtc tttcttcgcc gctttcggct tcgttttttt ctcaccgcgg aaggcgctgt
    51181 ctggctcaaa gtttaccttt ttaccgacct gacgacgctt gccgcctttt ttgcctgcat
    51241 cgcctttttt cgctttctcg cgcgccgttt taccgacgtt gcgcggtgcg cgttcactgg
    51301 agatcagact aaagtcgatt ttgcgctcgt ccatattaac cgcttcgact cgaacttcca
    51361 cgcgatcgcc caggcgataa gtctggccgc tggattcccc catcaggcgt tgccctacct
    51421 ggtcaaagcg atagtagtca ttgtccagcg aagagacatg gaccagacca tcaatgaaca
    51481 agtcgtccag acggacgaag aagccaaagc cagtgacgct ggaaattacg cctttaaaga
    51541 cgttacctac ctggtcgagc atgaagtcac acttcagcca gtcagcgaca tcgcgcgttg
    51601 cttcgtcggc acgacgttcc gccatcgaac agtgctgacc cagttgcaac atctcttcca
    51661 tcgaataatg gtagccgccg gtttcagtgg tgttgccctg atgcccctgc tctttcgcca
    51721 gcagatattt aatggcgcgg tgcagcgtca ggtcaggata acgacgaatc ggcgaagtaa
    51781 agtgcgcata ggactgcaat gccagaccga agtgaccacg gttttctgga tcgtaaatcg
    51841 cctgtttcat cgagcgcagc agcatggttt gcagcatttc tgcatcagga cggtcggcaa
    51901 ccgactccag cagttccgcg tagtcacgcg gttccggctt gttaccaccc ggcagctcca
    51961 gccccagctc cgccagcact gaacggaaag aggtaatcgc ttcggtgctc ggcttgtcgt
    52021 gaatacggaa cagtgccggt tctttcgctt tctcaacgaa acgcgccgcc gagatattcg
    52081 ccagaatcat gcactcttca attaatttgt gcgcgtcgtt acgctgggtc tgttcgatac
    52141 gttcaatacg gcgttcagcg ttgaaaatga acttcgcttc ttcgctctca aatgagatcc
    52201 caccgcgttc ttcacgggct ttatccagca ctttatagag gttatgcaac tcttcgagat
    52261 gcttaaccag cggggcgtac tgctcgcgca gatcctgatc gccctgcaga atatgccaga
    52321 ctttggtgta ggtcagacgc gcgtgagagc tcatcaccgc ttcgtagaat ttgtagcccg
    52381 tcaggcggcc tttcgacgaa accgtcatct cgcacaccat acacaggcgg tctacctgcg
    52441 ggttgagcga acacaggccg ttagagagca cttccggcag catcgggata acctgcgaag
    52501 ggaagtacac cgacgtgcca cggttacgcg cttctctgtc cagcggcgtt ggcggacgca
    52561 catagtagct gacgtcggca atcgcgaccc ataaacgcca gccgccgccg cgttttttct
    52621 cgcagtaaac tgcatcgtca aagtcacggg cgtcttcgcc atcaatggtg accagcggta
    52681 aatcgcgcag atcaacacgg cccgcttttg cttcttccgg cacttcttct ttcagcccgg
    52741 caacctgttg ctcaacagcc tgcggccaga tgtacggaat ttcatgggta cgcagagcga
    52801 tatcaaccgc catgccggtg cccatattgt cgcccagcac ttcgacgatt ttacccaccg
    52861 ctttggtgcg gcgagtcgga cgctgagtca gttcgactac gaccacaaag cccatccgcg
    52921 cgcccatgat ctgatcgggc gggattaaga tatcgaagct cagacggcta tcgtcaggaa
    52981 ccacaaagcc gacgcccgct tcggtaaagt agcgaccaac aatctggctg gtttttggca
    53041 ccagtacgcg gacaatacgc gcttcacgac gacctttacg gtcagcaccc agcggctgcg
    53101 ccagcacctg atcgccatga atgcaggttt tcatctgctc gctggagaga tacaaatcat
    53161 ctttacgccc ttcaacccgc agaaagccgt agccatcacg gtggccaata acggtacctt
    53221 tcaccaggtc gaggcgttcc ggcagcgcat agcactgacg acgagtgaag accagttgac
    53281 catcgcgctc catcgcgcgc aggcggcgac gcaggccttc aagctgctct tcgccttcaa
    53341 tgtgcagttc taccgccagc tcatcacggc tggccggttt ttcacgtttg gttaaatgtt
    53401 cgaggataaa ttcccgacta gggatgggat tcgcgtattt ttcagcttcg cgttcctgga
    53461 aaggatcttg tgacatctcg gttcctccgt tgtcatctct gatgaagatt ttcgtcactc
    53521 caccagcaat aatttataaa gcggttgatt ctcttcaacc aaatcggcaa gcgtgtagtt
    53581 atccagttcc gtaagaaaac tttgcacggc cttagaaagc gcctgtttca accgacaggc
    53641 aggtgtaatg tggcaaaact cactgctgca attcaccagc gataagggct ccagctcgcg
    53701 caccacatca ccaatacgta tcgcactcgc cggtttaccc aggcgaatgc cgccattttt
    53761 tccacgaaca gcagtcacgt agccggcacg actaagttga ttgattattt tgaccatatg
    53821 attacgggag acgccgtaga cgtcagtcac ttcagaaata ctggtcatcc gcccttctgg
    53881 caatgacgcc atgtagatca gcgcacgtaa tccgtaatca gtgaaactcg ttaactgcac
    53941 atcaacctca aaagggaaat cgggaaaaaa tacatttata ttgatgataa accagccaca
    54001 agctgtgtcg ctaatttatt tcagttcggg gaaggaaaaa agcgaggatt taacactgtg
    54061 tcggatagcg taatggctta accgacacag caatgtatca cactgttagc ccggcaggca
    54121 aaatatctgc caggcgtacc agaattacgc gtcgaacgga tcgcgcagaa tcatggtttc
    54181 agtacgatcc ggaccggtag agatgatatc gatcggcaca ccagtcagct cttcaatacg
    54241 cttgatgtag ttcagcgccg cctgcggcag accgctacga tctttcacgc cgaaggtgga
    54301 ttcagaccag cccggcatgg tttcgtaaat cggctctaca cctttccagt cgtcagctgc
    54361 cagcggagtg gtagtcactt cgcgaccatc cggcatacgg taagccacgc agagtttaac
    54421 ctctttcagg ccatccagaa cgtccagttt agtcaggcag aagccagaca gggagttcag
    54481 ctgtaccgca cgacgaacgg caacggtgtc cagccagccg gtacgacgac gacgccccgt
    54541 agttgcgccg aattcgttac cctgcttgca gaggaactcg ccagtttcat caaacagttc
    54601 ggtcgggaac ggacctgcac ctacacgagt tgagtaagct ttgaggatac ccagaacata
    54661 atcaacataa cgcgggccca ggccggaacc ggtcgccacg ccaccagcag tggtgttgga
    54721 agaagttacg tacggataag taccgtggtc gatatccagc agcgtaccct gcgcaccttc
    54781 aaacatgacg aaatcgccac gctgacgcgc ctggtcgagc aggtcagaaa cgtcaaccac
    54841 catagaagtc aggatgtcgg caactgccat cgtatcatcc agaactttct ggtaatcaac
    54901 cgcttcagct ttgtagtagt taaccaactg gaagttgtga tattccatca cttctttcag
    54961 tttttcagcg aaggtttctt tgtcgaaaag gtcgccaaca cgcagaccgc gacgtgctac
    55021 tttatcttca taagcaggcc cgataccacg accggttgtg ccgatcgctt tcgcgccacg
    55081 cgctttctca cgcgcgttat ccagcgcaac gtgataatca aggatcagcg gacatgcttc
    55141 agacagcagc agacgctcac gaacggggat gccacggtct tccagttctt tcatctcttt
    55201 catcagcgcg gccggagaca gcacaacacc gttaccgatg atgctggtta cattctcgcg
    55261 gagaatacct gatggaataa gatggagaac ggttttttca ccgttgatta cgagagtatg
    55321 gcctgcgttg tgaccgccct ggtagcgtac aacatattta gcccgttcag tcagaagatc
    55381 gacgatctta cctttacctt cgtcacccca ttgggtgccc agtacgacga cgttgttacc
    55441 catttttcaa aatcaccgtt tgcttaaaaa tggattctac catcgctttt tcagagttac
    55501 agcacttttt gcactcaaaa atgacctcga tcagtctgtt ttttgttcag ccaatcgttt
    55561 tcctcaacat gtagtagacc acaacgcccg caaccacaag tccaccgcca aaacgacgta
    55621 aaatattatc gggcaaattg gtcatcgcag agatcatctt cttccatgcc ttcgggtaaa
    55681 gcatcggccc taaaccttcc agtaccaaaa ccagggcaag cgccagccag attgtcgaat
    55741 tcattcatta tccttataga aaaagaaaac caccgacatc cctgaggatg cggtggcttt
    55801 attgacctgt accgcagtcg ttatattaac gcgttgcgga agtcggcgtc ttcatgtagc
    55861 ggaagaaatc gctatccggg ctcatgacca tcacgtcctg attgccagag aagctgttct
    55921 cataagcacg caggctacgg atgaatgcgt agaagtccgg atctttactg aatgcatcag
    55981 caaacagttt ggctgcttcg gcatcgcctt caccacgcat gatgcggccc tgacgctcag
    56041 cttctgccag cgttctggtc acttcatagt cggcagtcgc gcgcagtttt tccgcttctt
    56101 cctgaccttg tgaacggtga cgacgcgcta ccgcttcacg ctcggcgcgc atacggttgt
    56161 agatcgcttc agacacttcg gtcggcaggt tgatctgctt gatacgcaca tcgacaactt
    56221 caatacccag cgccgccata ctgttcgggt tgatgaccgg aactttgccc ttcgtctcag
    56281 ccgttacgcg ctctgccgct tcggcaatgg cgttatctgc cgccggggta gtaacttcat
    56341 cttctgtacc cgcagaaccg gagttcagcg cgtcacgtac ttcgagggtc agacgaccac
    56401 gggaatcggt gacgatgtct ttcacgtcca ggcgaccaat ttcagaacgc agacggtcag
    56461 agaacttacg tttcaacagc acttccgctt gcgaaatgtc gccaccaccc gttgccaggt
    56521 agtaacggct gaaatcgctg atgcgccatt tgatgtaaga gtcgacgatc aggtctttct
    56581 tctctttggt cacaaagcgg tcggcctggt tgtccatggt ctgaatacgt gcgtcgagca
    56641 ttttcaccgt ttcaatgaac ggtatcttga aatgcagacc cggctcataa accagaggtt
    56701 tgttgtcatc gtcacgcagt accttaccaa aacgcagcgt aataccgcgc tcaccttctt
    56761 tgacgacaaa gacagacatg taaagcacta ccagcacgat gatgataatc gcgataactg
    56821 acttacgcat cgttattccc cctgacgctg gtagtcgtta cgctgcgcgt tggcgcggcg
    56881 ttggtccata atatcgccct gactggtgga cgacgtgttg cttgctccac tggttgtgga
    56941 ggaagaggct ggcggcaaac gcagcagatt gctggcaccg ttatcgctct tcgccgcagg
    57001 ggcgttacca cctttcagca tctggtctaa cggcagaacc atcaggttgc cacctttatc
    57061 gttaaccagc actttgcggg tgttacccaa cactttttcc atcgtctcga tatacagacg
    57121 ctcgcgagta atttccggcg cggctttata ttccggcaga agtttagcaa agcgcgccac
    57181 ttcaccctga gcttccagga tggtctgggc cttgtacgca cgcgcctctt cgaggatacg
    57241 ttgcgcctga ccgttcgcac gcggctgaac ttcgttggta tacgcttctg cttcacgaat
    57301 gtattgctgt tcgttttcac gcgcggcaat cgcatcgtca aacgccgctt ttacttcttc
    57361 cggcggacga gcagcctgga agttgacgtc cagcagagtg atgcccatgt catacggacg
    57421 aatcgtctct tccagttcgc gctgggtatc gctacgaatc acggtacgac cttccgtcag
    57481 aatgcggtcc atggtgtatt taccgataac cccgcgcagg gcgctgtcgg tagcctgacg
    57541 caggctgtca tccgggctgg tcacgctata cagatatttt tccggattgg taacgcggta
    57601 ctgcacgttc atctcaacgc gcactacgtt ctcgtccgac gtcagcatca caccggaagc
    57661 ggccagttca cgcacggctt ccacgtttac cggtttgact tcgtcgataa acgtcggttt
    57721 ccagttcaga cccggctcaa ccagatggct gaatttacca aagcgtgtta ccacgccgcg
    57781 ttcggcttct ttaatggtat agaaaccact ggccgcccag ataatgacaa tcgctgccgc
    57841 tgcgatggta acgacacgac cgccaagctg cgggcgcggg ccttgcgatg aactgccacc
    57901 gccagatccg gtgcctttac cgccgcccag accaccgagc tttttgctca gtttgcggaa
    57961 gatatcatct aaatcaggtg gcccttgatc gcgaccgcct ttgtttccat ttccctcaga
    58021 gttgccgcca ggtttgctgc ttccccacgg gtcgcggtct tgtccgttat taccgggctg
    58081 attccacgcc atgtttgtgc tccatatttg ttatgcggtg atccctgttg tctttcgacg
    58141 ccagaggata tgactccacg cttcagacgc tacgccgtta gatcaggtaa tcgatcaacg
    58201 ccggttcttg tttacagagg cgacgccagt caacgatcgg catacgaact tgcagactta
    58261 cgctgccgtc ctcctccatc cactcttttt ctattgcctg aagctgataa aaacgacttc
    58321 tcagacgccc ttcctgcggt ggcagacgca atgtatgctg cgccacctcg ccggaaagcc
    58381 gctccgtcaa agcctgaaaa agctgtggta tccccgctcc ggtctgtgcg gaaagccaga
    58441 cacggatcgg tttgttctct tcgtcccgat caatacgcgg ttcgaaatct tccagcatat
    58501 cgatcttgtt catcaccagc agggttggga tctcgtgagc gtcgatctct tcaagaaccg
    58561 tattcaccgc ttcgatgttt tcttgtacac gcacatccgc cgcatcaatg acgtgcagca
    58621 gtaatgtggc ttgccgcgtc tcttgtaacg tggctttaaa tgccgccacc agatcgtgcg
    58681 gcaggtggcg aataaaccct acggtatctg caagtacggt ttcaccgaca tctgcaacgt
    58741 caatacgccg caacgtcggg tcgagggtgg caaacaactg gtctgccgcg tagacccgcg
    58801 cttcggtgat gcgattgaaa agggtagatt taccggcgtt ggtatatccc accagcgaaa
    58861 cagtaggaac gtcggctttg atacgcgatt gccgcccctg ctcacgctgc ttttcaactc
    58921 tttccaggcg tgactgtatc tgcacgatgc gattacgcaa caaacgacgg tcggtttcga
    58981 gctgggtttc acccggacca cgcaaaccta tcccgccttt ctgtctttca aggtgggtcc
    59041 agccacgcac caggcgcgta gccagatggc gcagctgcgc cagctcaacc tgcaacttac
    59101 cctcatgggt acgcgcacgt tgggcgaaaa tatctaaaat aaggccggtg cggtcgataa
    59161 cacgacactc gcacaaacgc tccaggttac gctcttgcgc cgggctcagg gcatggtcaa
    59221 aaagaacgac cgaagcaccc gtcgctttga cagcttccgc aatttcaact gctttacctt
    59281 cacctacaaa atactttggg tgcggcgctt tacggctacc ggtaatcacc tgcaatgctt
    59341 cgacaccggc ggaagagacc agagattcaa actcctggag gtcttccata tctttgtctt
    59401 gcgtaaaata gatgtgtacc agtaccgcct gctcaccagc atcataacgg tcaaacaagc
    59461 gtataaccct ctaaatagat cagcggggaa cgcaggatcg ctggctcccc gtgtaaaaaa
    59521 aacagcccga aaccttattc ggtttcttcg ctgtcctgtt gcgcggaagt attctgcgcg
    59581 ctgctaccat gatggtagtt actgctggta ccgccaccgg cgttgttact gtgatgagaa
    59641 accgggcgag acgggacaac agtagaaatc gcgtgcttgt aaaccatctg gctgaccgtg
    59701 tttttcaaca ggatcacgaa ctgatcaaaa gactcgattt gcccttgcag cttaatacca
    59761 ttcaccaaat aaatagaaac tggaacacgt tcccgacgca gtgcgttcag gaacggatct
    59821 tgtaaagatt gccccttagc cattctctct tttccttata tgcttatttg tactttgaac
    59881 ctttcgattc tgaaaaaatt gcgcacgata cgtctcaatt gtacacattc agcctgcgat
    59941 agcaccaaca acctgtaata cttcgtcacg cgcctgttct ggtttttcac tgtcaagcca
    60001 gtgaacccct tcccaaccac gcagccaggt tatctgccgc ttcgccaact gtctcgtggc
    60061 gcaaacacct cgataaacca tttcatcgta tgagatttcg ccttcaaggt aagaccacat
    60121 ctggcgataa cccacgcaac gaatggaagg caagtccgta tgcaaatctc ctcgggcaaa
    60181 aagcgcccgg acttctgctt caaaacctga agccaacatc tgatgaaaac gctgctcaat
    60241 gcgttgatgg agcagttcac ggctcgccgg ggcgatggcg aactgatgca cctgatacgg
    60301 tagagcgtct cctgacgttt gcgtcagttc cgttaaagtt ttacccgaaa tgaaaaaaac
    60361 ttccagtgcc cgggaaagcc tttgtggatc atttggatga atccttgctg ccgcaaccgg
    60421 atctacctcc tgaagttgac gatgcaatga ctcccaacct tgctctgccg cctgttgctc
    60481 aattctggcc cgcacttccg ggtctgccga cggtagcggc gacaaccctt ccagcaatgc
    60541 cttgaaatac aacatcgtac cgcccactaa cagtgggatc cgccccgccg cggtgatatc
    60601 ggccatttcc gccagcgcat cgcggcgaaa atcagcagcc gagtaagcct gcgacggatc
    60661 gcgaatatcc agcaatcggt gcggcgcggc gagtaactct tcagcgttcg gcttcgccgt
    60721 cccgatatcc atccctttgt aaataagggc agaatcaacg cttatcaact ctactggtaa
    60781 aattttacgc agctcaatgg ctaacgccgt tttaccggag gccgtcggcc ccatcaaaaa
    60841 aatcgcctta ggcaggctcg ccttactgat atcactcatc tttcagggct tttatcgccg
    60901 gatgtaaatc aacagattgt aacagaccac ccggcggcgt tttcacaagt tgcggacata
    60961 accgttccac gtccgccagc agggttatgg cctgtgccat tgaccactgc gcatgttcgc
    61021 tcatcagatt tcgtgcaatc cactgcgcaa tattgccagg ttcgaatacg gactgcttcg
    61081 ccaggtagcc tatcagttca ggaatcaaga tttgtaaatt ttgttggcgt aagggtaaag
    61141 gcacggccct gatggtcaca tgctgtgcat ctgactggaa atcaataccc aattccgcca
    61201 gggcagactg cgctttttct aatgccgatt tttcttcggc agaaactttt agccgcaacg
    61261 gaatcagcag cggctgggcg caaacgggcg cttcacccgg cgtcaattgt gcctgacgta
    61321 gccaacgttc tgccactggc aaggataaaa gtgaaatgtt gccatcgcgc tccagcaacg
    61381 cacagtcgga atggacgata gtcagtaccc gaccaaaact ctgactgttc gccgcaagtg
    61441 caggttcctg cggttccggc gcttttaatt tttgcatcgg cgcgggcgtt tgcaaaagct
    61501 ggcgatacac ttcaccttgc tgtttctggt agcctggctg cgcattcggc cagggggcag
    61561 ccggacgact gcctgatgct ggcgcaggag tgtagcgcgg agctaccggc tcacgagctg
    61621 ccggttctgc aaagtgattg cgccccgccg ccacgcggtt ttccgggatg gcacgcggtg
    61681 cgggttgtgg ttcatcgtcc agcggtagcg gcgtttccag ttgctgctgt agcacgctca
    61741 gcacgccctg atagataaag tcatgcacca gacgcgactg atggaaacgc acttcgtgtt
    61801 tggcggggtg tacgttgacg tccacctgat gcgggtcgat ctccagatac aacacaaacg
    61861 ccggttgttg atcggccccc agtttgtctt cgcaggcctg gcggatcgca tgattgatca
    61921 ggcgatcgcg catcatgcga ccattcacgt agcaatactg aatttccgcc agtgcgggcg
    61981 tagtgtgatt tggatcggcc acccagccgc gcagggtgag atcgccgtat tgccattcaa
    62041 tcgccagcgc ttgttcgaga aaagcggtgc cgcaaatcgc gcctaagcgc cgttcttttt
    62101 gcccgccttc cggcactgcg cggtactgac gcacaatttt accgttatgc gacaggttga
    62161 tcgtgacgtc gaaacgcgcc agcgcaatgc ggcggatgat ctcatcaatg tggttaaatt
    62221 cggttttctc ggtgcgcagg aatttgcgcc gcgccggggt gttgtagaac agatccagca
    62281 cctccagcgt cgtccccaca ggatgcgccg ccggttttac cgtcacgttc atatcgcgcc
    62341 cttcggcata ggcctgccag gcttcctgct gttctgcggt gcgtgaagtg agcgtcaggc
    62401 gggaaaccga actgatactc gccagcgcct caccgcgaaa gcccaggctg ataatggctt
    62461 cgagatcgtc cagagaggcg attttactgg tggcatgacg cgccagcgcc agcgccagct
    62521 cgtctttttt gataccgcag ccgttatcac gaatgcggat aagtttcgcc ccaccgcgtt
    62581 cgatatcaat atcgatacgt gtcgcacctg catcgaggct gttttccacc agttctttga
    62641 ctaccgacgc aggtcgctcg accacctcac ctgcggcaat ctggttcgcc agttgtggcg
    62701 gtaagacctg aattggcatc aatcgtcctt agtttggcag cgtgcgatct ggcgtcgtca
    62761 ccgtactggc cgtttgtgcc gttgccccct gcggcgcaga ttgcatcgga tgcgcaagga
    62821 aataattgcg caggcctttg taaatggctt ctgccagctg ttgttggtaa tcgtcgctcg
    62881 ccagcaaacg ttcttcgctg ttgttgctga taaaaccggt ttcgaccagt actgatggga
    62941 tatccggcga acgcagaacg ccgagactcg cgtgttctgg tcggcgttta tgtatttcgc
    63001 caatgcgttg caactgactg atcatactgg tcgctacatc ataccctacc cgttgggaat
    63061 gaccgaactg taaatccagc accgcctggc ttaaataggg gtcagactga ctgttcgcca
    63121 gcacatcacc cgccccaccc agcagctccg actgtttctc gtgctgctcc agccagctgg
    63181 ccatttcgct gttggcgcga cggttagaga gcacccatac ggaagcgcca gtcgcactgc
    63241 ggttcggtgc ggcatcagcg tgaatcgaca cgaggaaatt ggcgttttgc ttacgtgcca
    63301 catcactgcg ccccatcacc gagataaagt aatccccgtc acgggttaaa acgcccttaa
    63361 acatcggatc gtcattgagc aaagtacgca atttacgcgc gatggcgatg gtgacatttt
    63421 tctcccgcgt accaccgggg ccgatagcgc cagggtcctg accgccgtgt ccggcatcaa
    63481 tagcgatgat aattttatcg ccagtgttag ccgtcgcgcg cgctgccgga cgcgttaccg
    63541 tattactgct gataacaccc gtagtgcggt tactttccgt tttaaacgga ttgcgcgccg
    63601 gttcgctgac gcgcggtgcg acaaccgcag gcgtttcaac gcgtttcgca accacgggcg
    63661 gcggaggagg aggtggcggc gcatcggcgt taatcgtaaa gacgacagtg taattgctgc
    63721 cattctgccg cttcaccgct tcggttttac cgttttcggt aagatcgacc accagccgta
    63781 gcgtttgtgc atctttaggc gttccagagc gaatcgcctt caccagatta ttgccgctga
    63841 acaacaacgg cagtccctga atcacgcccg tttgtttgat atcgagcgcc acggtgcgtt
    63901 tgctttgatg gctaaacgca taatcaggat cgccaataaa actcaacgtt atccgcgcct
    63961 gttgattacc gttagaaacc tgaatatcag agagcgtcgc ggcacccacc ggcgtgcaca
    64021 gcagcagcag cgtcgctacc aaccaatttc tgatgcgata catcatcccg ccacctttca
    64081 aaggttaacc ggctaaacgc gccagcaaca attcacccgc agaggaaacc gcactcacgc
    64141 gcgcctcacg gccttgtgcc tgataatcaa tgtgtatttc gacatccggg tcaggaagaa
    64201 cacctgtacc ttgttgtggc cactccacca ggcagatggc atcgttggca aaataatcgc
    64261 ggatccccat aaactccagc tcctcgggat cggcaaggcg gtacaaatca aagtgataaa
    64321 ccattaaatt gtcgagcgta tagggttcga ccagcgtata agtggggctt ttgacattac
    64381 cctgatgacc cagagcttgt aaaaagcccc ggctaaaggt ggttttacct gcgcctaaat
    64441 cgccatacag atagattacg gttgcgccat cgcaggcttt cgctacccgc tcgcccaggt
    64501 ctaatgttgc ctgctcatca gggagcggaa ttactcgatt catcatggtt tttatcagtc
    64561 acttccgggt taacaatacg ctgtagcgtg gaaaagagat cggttgccag catcccgcgc
    64621 gttccaaaac gcgccgccag tacgtcagct gcagcaccgt gcgcgacaca gcccgcacag
    64681 gcggcatcat acggcgacag tttttgccca agcaatgcgc caataatacc agagagcaca
    64741 tcgcccatgc cgccgctcgc catgcctgca tttccggcat caataatgcc taaagcgtca
    64801 ggatgggcgg cgaccacggt tccggcacct ttcagcaccg ctacgccgcc ataacgttgt
    64861 accagacgtt tggcgcaatg taagcggtca ctttcaattt cagcgacgga acagcctaac
    64921 aaccgtgcgg cctcgccagg atgcggcgtg atcacgcgat tgtgacgctt atcgggattg
    64981 attgccagca ggttcaatgc atcggcatcc cacaacatcg gtttgcgaaa attctcaact
    65041 ttttgcagtg cttttttccc ccactcttgc tggcccagac cgggaccaat caccaccaca
    65101 tcggcccatt ccaggctttc ggtaagagag tccatcgtca gttcatgcac catcaattcc
    65161 ggtcgtgcag tcagcagcgg cgcaatgttt tcactgcggg tcagtactcg gactaaacca
    65221 gcaccagcac gtagcgccgc ttcccccgtc atacgaatag cccccgccgt gccgtgatcg
    65281 ccaccgataa ttaccagccg cccgtgatcg cctttatgcg aagtcgggcg acgcggtttt
    65341 agccagtgag aaagttgttc tgccgaaaac cgctgaattt tcgtctcctg acctgccagc
    65401 caactatcca gccccagtga gtcaaaatgc agttgtccgg taacatcccg cgcttttcca
    65461 gtgagcaagc ctggtttcag cgcaataaaa gtgatggtgt gatcggcgtt gatcactgcg
    65521 cctggcgtag cgccggtttc agccagcagg ccggaaggga tatcaaccgc cgcaatcggc
    65581 gcaggatggg aattagcgtg gtcgattaac tggctaatgg attcgcgggg cgcttgctgc
    65641 aagccggtac cgagcagcgc atcaacaatc agatctaccg attcgggcca gacaatattc
    65701 gaagcatgga tctcgcctcc cgcgtttaac catgcttcgc gtgccagcgc ggcctcttcc
    65761 ggcaacggtt tgtcgctctc ctgggccagc agcgtgacct caatgccgac cgctgtggcc
    65821 agccgcgcga ccacgtagcc atcgccgccg ttattaccat gaccgcacag caccagccaa
    65881 tggcgggcgt caggatacgc cgaacgacac acctggaatg cggcttcgcc agcgcgaagc
    65941 atcagctcat agagtgtaag ccccagcgca tctgccgcct cacgttctcc gcggcggata
    66001 tcgtcggcgt gccagacggt gtgtggtata cttacggggt ttttcttcat tgtatggtcc
    66061 gtcatgtcag agcccctcga tctcaatcat ttagcgcaaa aaattaaaca gtgggggctg
    66121 gaactgggct ttcagcaggt aggtattacc gataccgatc tcagcgagtc cgagcccaaa
    66181 ctgcaagcat ggctggacaa acaataccac ggcgaaatgg actggatggc acgtcacggt
    66241 atgctgcgcg ctcgccccca tgagttattg cccggtacgc tgcgcgtgat cagcgtgcgg
    66301 atgaattacc ttcctgctaa cgccgcattt gccagcacgc tgaaaaaccc caaactcggc
    66361 tatgttagcc gttatgcgct gggccgtgac tatcacaaac ttctgcgcaa ccgactcaaa
    66421 aagctgggcg agatgattca gcaacattgt gtttcgctga attttagacc gtttgtcgat
    66481 tctgcgccta ttctcgagcg cccgttagct gaaaaagctg ggctcggctg gacaggtaag
    66541 cactcactta tcctcaatcg cgaggccggt tctttcttct ttttaggcga attgctggtc
    66601 gatattccgc tgcccgtgga tcaaccagtc gaggaaggat gcggtaaatg cgtggcctgt
    66661 atgacgattt gcccgaccgg tgccatcgtc gagccatata ccgtcgatgc tcgccgctgt
    66721 atctcttatc tcaccatcga acttgaaggg gcgatcccgg aagagttgcg accgttaatg
    66781 ggaaaccgta tttacggttg cgatgactgc cagcttatct gcccgtggaa tcgctattca
    66841 caactcacca cagaagagga tttcagcccg cgtaagccgc tacacgcacc ggaactcatt
    66901 gagttattcg cctggagcga agagaagttt ttaaaagtca cggaaggatc ggcgattcgt
    66961 cgtattggtc acctgcgttg gctgcgtaat atcgccgtag cattaggcaa tgccccttgg
    67021 gatgaaacga ttttgacagc gctggaaagt cgtaaaggtg agcacccact tctcgatgag
    67081 cacatagcgt gggcgattgc gcagcaaata gagagacgaa atgcgtgcat agtcgaagtg
    67141 caattgccga aaaaacagcg tctggttcgg gtgattgaaa aagggttacc gcgtgacgcc
    67201 tgattcatcc acagcttgtg aataaaaata aaaacgcatt gcaattcaag acgaataaaa
    67261 tcgtcaagtg atcgactcga caaaacggaa tcttaatttt taacttatct atcaataggt
    67321 taaaaaaata ctattcaccg tgcgaagttt ctttgaaatt aagattacag cctgaggctg
    67381 tggataactc tgtttacaag agtttgtcag agacaacgaa aaccagcaat aacgcatcgc
    67441 atcgctgtgg atattttatt gagagaagaa tttggagcgg gaaacgagac tcgaactcgc
    67501 gaccccgacc ttggcaaggt cgtgctctac caactgagct attcccgctt gggtggtctg
    67561 tgccttacag cactttcaaa ttttggagcg ggaaacgaga ctcgaactcg cgaccccgac
    67621 cttggcaagg tcgtgctcta ccaactgagc tattcccgct tgggtggtct gtgctttgca
    67681 gcacttttca aattttggag cgggaaacga gactcgaact cgcgaccccg accttggcaa
    67741 ggtcgtgctc taccaactga gctattcccg cgtactactt aattttgctt tcgtaatttt
    67801 cgcatttctg cgtcgttacg ggaggcgcat tatacgagaa atctttccct ctgcaacccc
    67861 ccaaaaaagt tttttttgcg tttttatctc aagtgctgaa attatcagca agacgttcaa
    67921 tttagcgtca aaatcacgcc gccgcaagtg gattccggcg cttacgtgac caggaaaaat
    67981 tttacagctt gataaaatgc tc
//
LOCUS       DAMZET010000008        67493 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.008, whole genome shotgun sequence.
ACCESSION   DAMZET010000008 DAMZET010000000
VERSION     DAMZET010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.008"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(151..864)
                     /gene="livF"
                     /locus_tag="POB34_000625"
     CDS             complement(151..864)
                     /gene="livF"
                     /locus_tag="POB34_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HDI5917624.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(866..1633)
                     /gene="livG"
                     /locus_tag="POB34_000626"
     CDS             complement(866..1633)
                     /gene="livG"
                     /locus_tag="POB34_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HDI5917625.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(1630..2907)
                     /gene="livM"
                     /locus_tag="POB34_000627"
     CDS             complement(1630..2907)
                     /gene="livM"
                     /locus_tag="POB34_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HDI5917626.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(2904..3830)
                     /gene="livH"
                     /locus_tag="POB34_000628"
     CDS             complement(2904..3830)
                     /gene="livH"
                     /locus_tag="POB34_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HDI5917627.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTVFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(3878..4987)
                     /gene="livK"
                     /locus_tag="POB34_000629"
     CDS             complement(3878..4987)
                     /gene="livK"
                     /locus_tag="POB34_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HDI5917628.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            5411..5794
                     /gene="panM"
                     /locus_tag="POB34_000630"
     CDS             5411..5794
                     /gene="panM"
                     /locus_tag="POB34_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HDI5917629.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQGDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLCVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(5791..6318)
                     /locus_tag="POB34_000631"
     CDS             complement(5791..6318)
                     /locus_tag="POB34_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5917630.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
                     FFPTKSIELLFTQSD"
     gene            complement(6325..6591)
                     /locus_tag="POB34_000632"
     CDS             complement(6325..6591)
                     /locus_tag="POB34_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDI5917631.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
     gene            complement(6741..7844)
                     /gene="livJ"
                     /locus_tag="POB34_000633"
     CDS             complement(6741..7844)
                     /gene="livJ"
                     /locus_tag="POB34_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HDI5917632.1"
                     /translation="MNMKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(8116..8970)
                     /gene="rpoH"
                     /locus_tag="POB34_000634"
     CDS             complement(8116..8970)
                     /gene="rpoH"
                     /locus_tag="POB34_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HDI5917633.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(9215..10273)
                     /gene="ftsX"
                     /locus_tag="POB34_000635"
     CDS             complement(9215..10273)
                     /gene="ftsX"
                     /locus_tag="POB34_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HDI5917634.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(10266..10934)
                     /gene="ftsE"
                     /locus_tag="POB34_000636"
     CDS             complement(10266..10934)
                     /gene="ftsE"
                     /locus_tag="POB34_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDI5917635.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(10937..12430)
                     /gene="ftsY"
                     /locus_tag="POB34_000637"
     CDS             complement(10937..12430)
                     /gene="ftsY"
                     /locus_tag="POB34_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDI5917636.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLF
                     RGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMG
                     EILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFRA
                     AAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSH
                     LMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDG
                     TAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            12580..13176
                     /gene="rsmD"
                     /locus_tag="POB34_000638"
     CDS             12580..13176
                     /gene="rsmD"
                     /locus_tag="POB34_000638"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HDI5917637.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            13166..13435
                     /locus_tag="POB34_000639"
     CDS             13166..13435
                     /locus_tag="POB34_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HDI5917638.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(13438..13797)
                     /locus_tag="POB34_000640"
     CDS             complement(13438..13797)
                     /locus_tag="POB34_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HDI5917639.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            13938..14564
                     /locus_tag="POB34_000641"
     CDS             13938..14564
                     /locus_tag="POB34_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HDI5917640.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            14638..16836
                     /gene="zntA"
                     /locus_tag="POB34_000642"
     CDS             14638..16836
                     /gene="zntA"
                     /locus_tag="POB34_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HDI5917641.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQATDEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(16938..17183)
                     /gene="tusA"
                     /locus_tag="POB34_000643"
     CDS             complement(16938..17183)
                     /gene="tusA"
                     /locus_tag="POB34_000643"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HDI5917642.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            17404..18069
                     /locus_tag="POB34_000644"
     CDS             17404..18069
                     /locus_tag="POB34_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HDI5917643.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            18142..18699
                     /gene="dcrB"
                     /locus_tag="POB34_000645"
     CDS             18142..18699
                     /gene="dcrB"
                     /locus_tag="POB34_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HDI5917644.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(18703..19920)
                     /locus_tag="POB34_000646"
     CDS             complement(18703..19920)
                     /locus_tag="POB34_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5917645.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     SVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            20052..21101
                     /locus_tag="POB34_000647"
     CDS             20052..21101
                     /locus_tag="POB34_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDI5917646.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            21156..21743
                     /gene="acpT"
                     /locus_tag="POB34_000648"
     CDS             21156..21743
                     /gene="acpT"
                     /locus_tag="POB34_000648"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HDI5917647.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            21854..23428
                     /gene="nikA"
                     /locus_tag="POB34_000649"
     CDS             21854..23428
                     /gene="nikA"
                     /locus_tag="POB34_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HDI5917648.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            23428..24372
                     /gene="nikB"
                     /locus_tag="POB34_000650"
     CDS             23428..24372
                     /gene="nikB"
                     /locus_tag="POB34_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HDI5917649.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            24369..25202
                     /gene="nikC"
                     /locus_tag="POB34_000651"
     CDS             24369..25202
                     /gene="nikC"
                     /locus_tag="POB34_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HDI5917650.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            25202..25966
                     /gene="nikD"
                     /locus_tag="POB34_000652"
     CDS             25202..25966
                     /gene="nikD"
                     /locus_tag="POB34_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HDI5917651.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            25963..26769
                     /gene="nikE"
                     /locus_tag="POB34_000653"
     CDS             25963..26769
                     /gene="nikE"
                     /locus_tag="POB34_000653"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HDI5917652.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            26775..27176
                     /gene="nikR"
                     /locus_tag="POB34_000654"
     CDS             26775..27176
                     /gene="nikR"
                     /locus_tag="POB34_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HDI5917653.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(27296..27655)
                     /locus_tag="POB34_000655"
     CDS             complement(27296..27655)
                     /locus_tag="POB34_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDI5917654.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(27652..27927)
                     /locus_tag="POB34_000656"
                     /pseudo
     CDS             complement(27652..27927)
                     /locus_tag="POB34_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            complement(27986..29110)
                     /locus_tag="POB34_000657"
     CDS             complement(27986..29110)
                     /locus_tag="POB34_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5917655.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(29110..31845)
                     /gene="rbbA"
                     /locus_tag="POB34_000658"
     CDS             complement(29110..31845)
                     /gene="rbbA"
                     /locus_tag="POB34_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HDI5917656.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(31842..32909)
                     /locus_tag="POB34_000659"
     CDS             complement(31842..32909)
                     /locus_tag="POB34_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDI5917657.1"
                     /translation="MDKSKRHLAWWVVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAHRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(33275..34897)
                     /locus_tag="POB34_000660"
     CDS             complement(33275..34897)
                     /locus_tag="POB34_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HDI5917658.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSDMRAHILRR
                     DNNYQFIVTINSKNYGCYLDNTDINWCSIVYLLNNMTVNDDANDVAVTESYKPVWDWK
                     ISQFNVSDIKFETMINPQFADRTYFSNCSPVDPTGTRPTYFGDTDGSVGAVLYALFAT
                     GHLRIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYAGIKDYPVCFYDPKYKIMANHHGITFDEQRKRYIIGPIT
                     VSIDEMTNALDPVELAAIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(35159..36748)
                     /locus_tag="POB34_000661"
     CDS             complement(35159..36748)
                     /locus_tag="POB34_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HDI5917659.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKTHRAKIVEFD
                     KLDNDSQIDNDFGLHIIYFLQHGHWKVNYRSHQMEKVWFYNSEPSIDIQEYNRFADNT
                     TDTFIFTIIPDNNHVLKLSSPITVTVECKGGYYFINSSGDKSDIIYKVDGLSIIARNF
                     FTLLSGNFKPDWRWDVSKETFTKEKFDSYVKPVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHEEMASSAYKHKNNKVLYDLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVVVLAGNHEINFNGNYTARLANHKLSAGDTYNLIKTLDVCNYDSERQVLTSHHGI
                     IRDEEKKCYCLGALQVPFNQMKNPTDPEELANIFNKKHKEHMDDRFIHLIRSNAIAST
                     PVYDNYFNNTTAFRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPH"
     gene            37132..38183
                     /locus_tag="POB34_000662"
                     /pseudo
     CDS             37132..38183
                     /locus_tag="POB34_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
     gene            complement(38498..39700)
                     /locus_tag="POB34_000663"
     CDS             complement(38498..39700)
                     /locus_tag="POB34_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDI5917660.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            39932..41431
                     /gene="pitA"
                     /locus_tag="POB34_000664"
     CDS             39932..41431
                     /gene="pitA"
                     /locus_tag="POB34_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HDI5917661.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGEIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(41502..41837)
                     /gene="uspB"
                     /locus_tag="POB34_000665"
     CDS             complement(41502..41837)
                     /gene="uspB"
                     /locus_tag="POB34_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HDI5917662.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            42228..42662
                     /gene="uspA"
                     /locus_tag="POB34_000666"
     CDS             42228..42662
                     /gene="uspA"
                     /locus_tag="POB34_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HDI5917663.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            42980..44449
                     /gene="dtpB"
                     /locus_tag="POB34_000667"
     CDS             42980..44449
                     /gene="dtpB"
                     /locus_tag="POB34_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HDI5917664.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(44498..45250)
                     /gene="rsmJ"
                     /locus_tag="POB34_000668"
     CDS             complement(44498..45250)
                     /gene="rsmJ"
                     /locus_tag="POB34_000668"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HDI5917665.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(45258..47300)
                     /gene="prlC"
                     /locus_tag="POB34_000669"
     CDS             complement(45258..47300)
                     /gene="prlC"
                     /locus_tag="POB34_000669"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HDI5917666.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            47503..48345
                     /gene="rlmJ"
                     /locus_tag="POB34_000670"
     CDS             47503..48345
                     /gene="rlmJ"
                     /locus_tag="POB34_000670"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HDI5917667.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            48417..49769
                     /gene="gorA"
                     /locus_tag="POB34_000671"
     CDS             48417..49769
                     /gene="gorA"
                     /locus_tag="POB34_000671"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDI5917668.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(49823..49906)
                     /locus_tag="POB34_000672"
     CDS             complement(49823..49906)
                     /locus_tag="POB34_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HDI5917669.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            50646..50999
                     /gene="arsR"
                     /locus_tag="POB34_000673"
     CDS             50646..50999
                     /gene="arsR"
                     /locus_tag="POB34_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HDI5917670.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            51053..52342
                     /gene="arsB"
                     /locus_tag="POB34_000674"
     CDS             51053..52342
                     /gene="arsB"
                     /locus_tag="POB34_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HDI5917671.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLVALALRLSFTL"
     gene            52355..52780
                     /gene="arsC"
                     /locus_tag="POB34_000675"
     CDS             52355..52780
                     /gene="arsC"
                     /locus_tag="POB34_000675"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HDI5917672.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGITVRALLRKNVEPYEELGLAEDKFTDERLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            53405..54520
                     /locus_tag="POB34_000676"
                     /pseudo
     CDS             53405..54520
                     /locus_tag="POB34_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            54875..55441
                     /gene="slp"
                     /locus_tag="POB34_000677"
     CDS             54875..55441
                     /gene="slp"
                     /locus_tag="POB34_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HDI5917673.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            55597..56127
                     /locus_tag="POB34_000678"
     CDS             55597..56127
                     /locus_tag="POB34_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI5917674.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(56169..56816)
                     /locus_tag="POB34_000679"
     CDS             complement(56169..56816)
                     /locus_tag="POB34_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDI5917675.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(56880..57206)
                     /gene="hdeB"
                     /locus_tag="POB34_000680"
     CDS             complement(56880..57206)
                     /gene="hdeB"
                     /locus_tag="POB34_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HDI5917676.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            complement(57322..57654)
                     /gene="hdeA"
                     /locus_tag="POB34_000681"
     CDS             complement(57322..57654)
                     /gene="hdeA"
                     /locus_tag="POB34_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HDI5917677.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            57909..58481
                     /gene="hdeD"
                     /locus_tag="POB34_000682"
     CDS             57909..58481
                     /gene="hdeD"
                     /locus_tag="POB34_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HDI5917678.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            59280..59807
                     /gene="gadE"
                     /locus_tag="POB34_000683"
     CDS             59280..59807
                     /gene="gadE"
                     /locus_tag="POB34_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HDI5917679.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(59808..60086)
                     /locus_tag="POB34_000684"
     CDS             complement(59808..60086)
                     /locus_tag="POB34_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917680.1"
                     /translation="MRDLQTSGIVGLSASKVGYRYSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            60146..61303
                     /gene="mdtE"
                     /locus_tag="POB34_000685"
     CDS             60146..61303
                     /gene="mdtE"
                     /locus_tag="POB34_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HDI5917681.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            61328..64441
                     /locus_tag="POB34_000686"
     CDS             61328..64441
                     /locus_tag="POB34_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDI5917682.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLIKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(64804..65532)
                     /gene="gadW"
                     /locus_tag="POB34_000687"
     CDS             complement(64804..65532)
                     /gene="gadW"
                     /locus_tag="POB34_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HDI5917683.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(65900..66724)
                     /gene="gadX"
                     /locus_tag="POB34_000688"
     CDS             complement(65900..66724)
                     /gene="gadX"
                     /locus_tag="POB34_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HDI5917684.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            complement(67094..>67493)
                     /locus_tag="POB34_000689"
     CDS             complement(67094..>67493)
                     /locus_tag="POB34_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDI5917685.1"
                     /translation="GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAV
                     CFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAEL
                     LLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
BASE COUNT        16560 a        17447 c        16418 g        17068 t
ORIGIN      
        1 gcgtaaacgc cttatccggc ctacaagatc gtgcaaattc aatatattgc aacccaccca
       61 gtaggcctga taagcgcagc gcatcaggca attttacatt tgtcacctgt ctcaaagaag
      121 tcttttgact ccttatcaat caacgtgtta ttacccgcct aaatacgcac ttctcaccgc
      181 ttcgttcgcc agtagtgcat caccagtatc ggagagcacc acatggccgt tttccagcac
      241 gtagccgcga tccgccagct ttagcgcctg gttggcgttc tgctcgacga ggaagatagt
      301 catcccctgc tcgcgcagct gctcgatggt gtcgaaaatt tgctggatga taatcggcgc
      361 aagaccgagc gatggctcat caagtagcag caaacgcggg ttgctcatca gcgcgcgacc
      421 aatcgccagc atctgctgtt caccgccgga catggtgccc gcccgttgaa cacggcgctc
      481 atggagacgt ggaaacagct catacaccca ctttatgcgc tcctgaaact gatcacgttc
      541 ggcaaaaaag ccgcccatcg ccaggttctc ttccaccgtc atccgcgaga agacgcgacg
      601 cccttccggg acaatcgcta ccgcttcgcg catgattttc gccgtttgcc agtcggtaat
      661 gtctttatca tcaaacacaa ttcgcccgct ggtggcacgc ggatcgccgc ataacgtgcc
      721 gagcaaggtg gttttccccg ccccgttcgc accaatgagg gtgacaatct cgccctgatt
      781 gatatgcagg ctcacctcat gcagcgcctg gattttgccg tagtgggcgc tgactttgtc
      841 aaaggacaac atgacttttt ccatcttatg cctcacctaa ataggcacgg atcacgtccg
      901 ggttattacg gatttgctcc ggcgtaccgt ttgccagtgg cgtcccctga ttgaccacgt
      961 aaattcggtc cgaaattccc atcaccagct tcatatcgtg ttcaatcaac aagatagtgg
     1021 tgttgtgatg attgcgcagt tcggcaatca gctcatccag ctctttcgtc tctttcgggt
     1081 taagacctgc cgccggttcg tcgagcatta aaatctccgg ctgcgtcacc atgcagcggg
     1141 caatctccag acggcgttgg tcaccatagg ccaggttact cgcctgacgg ttggcgtgtt
     1201 ccagcaaacc aatgcgctca agccaggtgg cggcgcggtc gagcgcttcg ctctgggcgc
     1261 gacggaaaga tggcgttttc aacaggccag agaacagccc ggttttcagt tgctgatgct
     1321 gcgccaccag caggttttca attaccgtca tttcacggaa cagacgcaca tgctggaagg
     1381 tacgcaccac gcccatgcgg gcaatttgct gccccggtaa accttccaga tgctgatcac
     1441 gcagtaaaat agtgccgccg gtgggtttgt agaatccggt caggcagtta aaaaccgtgg
     1501 tttttccggc accgttaggg ccgattaacg agacgatctc ctgcgggtac agttcaagat
     1561 tgacgttgtt caccgccagc aagccgccga agcgcatcat cagaccgtta acagataata
     1621 atggctgact catgcctgct ctcctttcgc tgcgccgttt ttcagcttca gttgcgggcg
     1681 cgtcatgggc agcaagccct gcggacgcca gatcatcatc agcaccatca aaccaccgag
     1741 cattaacatg ctgtattcgt tgaaatcacg catcaactcg cgcgacacca ccagcaaaat
     1801 tgccgccaga atcaccgcaa attgcgagcc cataccgccg agcaccacta tcgccagcac
     1861 aaacgccgat tcggcaaagg tgaaggattc cgggctgaca aagccctgac gcgcagcaaa
     1921 cagcgttccg gcaaaaccgg caaacgcggc actgatggta aaagcggtca gcttgatacg
     1981 acgcgggctt aaacccagcg aacggcaggc gatctcgtct tcacgcaacg cttcccacgc
     2041 acgccccagc ggcatccgca gcaggcggtt aatgacaaac agacttaata ccaccagcag
     2101 caacgccacc aggtagagga agatgacacg atcggaggga tcgtatttca ggccaaagaa
     2161 attactgaac gtgtcccagc cgccttcacg agcggtacgg ctgaactcga gtccgaagag
     2221 tgtcggtttc gggatctgac tgattccgtt cgggccgccg gtaatttcgg tattattgag
     2281 caacaatatg cgcacaattt cgccgaaacc gagggtaacg atcgccagat agtcaccgcg
     2341 caagcgaagc accggaaagc cgagcaggaa gcccgccgcc gcggccatta acccggcaat
     2401 cggcagacag gtccagaagc ccaggccgta atagtgattg agcagcgcaa aagtgtaagc
     2461 gccgatggcg taaaagccgc cgtaccccag caccagcaga ccagaaagac caacaaccac
     2521 attcagcccg agaccgagga taatgtagat catggtcaga gtggcgatat ccaccgtccc
     2581 acgtgacacc ataaacggcc acgccaccgc aagcaccagc agcgccacga ggaacagttt
     2641 ctgcttcacc gtggagccat caatcgcggg cagaataaac ttcggtccgg aaacactttt
     2701 caaccctttc tggaaagcag gtcgcaaaag ctgaaagaaa aagaccaccg ccgtgccgat
     2761 aaacacccac tgccaacgga tgtctgaagc cgtgtcgacc accagtttgg tgccatccag
     2821 ctccagttgc acgcccataa agacgcccgc caggacaaaa aacatcgcgg cagagagcag
     2881 cgccattgca atatgcatcg gtttcatact ttctctacct ccgggcgacc cagaataccg
     2941 gtcggcatca ccagcagcac cagaatcagc agggcgaatg agaccacatc tttatattcc
     3001 gtactcagat aggcagaaga gagcgcctcc gcaatcccca gaatcagccc gccaatcatc
     3061 gctcccggaa tgctgccaat cccaccgagc accgcagcgg taaaggcttt catcccggcc
     3121 ataaagccga tgtaggggtt aattacgccg tagaactgac cgagcagcac acctgccacc
     3181 gccgccatcg ccgcgccaat cacaaaggtc agcgcaatca cccggtcggt gttaatgcca
     3241 agcaggctcg ccattttcag gtcttccgcg caggcacgac acgcgcgccc catacgggaa
     3301 tagcgaatga aaaccgtcag cgccagcatg gcgaggaagg tgacaatcca gatcaccgcc
     3361 tgcatggtgg taatagaggc agagaagttt tcgctatgcc ccaccaccca ctgaccgtta
     3421 aacagactgg gcagcgccac gtcacgcgaa ccttcagtca ggctaacgta gttttgcagg
     3481 aagatggaca tgccgattgc cgagatgagc gcaatcaggc gcttagagtt acgcaccggg
     3541 cgataggcta cccgttcgat actccagccg taggcgctgg cgatgacgat tgcgccgacg
     3601 aatcccgccg ccaccagcag ccagccggta tcaatgccca tcatcatcag cgcggcgatg
     3661 atcataaatg agacatagct gccgatcata taaacctcgc cgtgggcgaa gttgatcatg
     3721 ccgataatgc cgtaaaccat ggtgtagccg atggcaatca gcgcgtaggt actgcccagc
     3781 gtgacgccgt taaacatctg ctgcaagaaa tataaaaact gctcagacat aaggtaacct
     3841 ttctaaaccc gcccgttttt cacgggcggt gggatgatca cttggctgcc gtggatgaac
     3901 cgtcggcgtg ccactggaag acaccaaaat caaatccctt aagatcgcct ttttcatccc
     3961 agttcagcgg cccaatcacg gtgtttgcac cgttagcttt taaatctttc accagcgcca
     4021 gcggctcatc gctgccagta cgctcaaggg cagtcgccag agattgcacc gccgcgtagg
     4081 tgatccagac ataaggcccg gacggatctt tcttgtctgc tttcagcgca tcaacgatgc
     4141 cctggtttgc cggatcctgg tcatagcgtt ttggcatagt gaccaacatg ccttcggcag
     4201 catcgccagc aatattcgac aatgatgcgt tacccacacc ttccggcccc ataaactgag
     4261 ttttcaggcc aacggaacgg gcctggcgca gcatctgccc catttccggg tagtaaccgc
     4321 cgtagtaaac gaagtcgatg ttttcttttt tcaggcgggc gatcagcgcg gagaaatctt
     4381 tctccccggc ggtaataccg tcgaagaaga cgacgttggc gttagccgct ttcagcccgt
     4441 cctgcaccga acgcgccagc ccttcgccat actgttgttt gtcgtgaatg atggcgatgc
     4501 gctggggctt caccgtctca agaatgtatt ttgccgccgt aggcccctgg gaagagtcca
     4561 gcccggcagt acgcataatg tgttgataac cgcgttgggt cagctccggg ttggtcgctc
     4621 ccggcgagat catcagaata ccttcgtctt cgtagatatc tgacgcaggt tgggtagaag
     4681 aagaacacag atgaccaata acgtatttaa tgccgtcgtt aacgattttg ttggcgaccg
     4741 caacggcttg tttcgggtcg caggcgtcgt catattccac gcccaccagc ttatcgcctt
     4801 taattccccc tttggcatta atgtctttaa tcgcctgacg cgcgccgtta aattccatat
     4861 cgccccactg ggcaatcggg ccggacatcg cgccgacaac ggcaacttta atatcgtcag
     4921 ccatagcggt gtgtgaaatt gccagtgcaa tcatccctgc gatgatagtt ttcgcattcc
     4981 gtttcatagt caaaaatccc cattcgtgat gttgtgttgc tttgttttta tgtgttaaca
     5041 aatcagactg ttcttttttt atactgcact gtttttgcct gtctgattaa gggtttagcg
     5101 cagtattttg tgataatagc gattaaaatc cctatttttc agtcgattaa gaacagataa
     5161 tattctgaat ttattgatag ataaacagaa aaaagcgccg ttgtcagcat aaaataacgg
     5221 tacaaagagc ggaataattc actaccattc aggggattat gctgggtatt tttcattctc
     5281 taatgtttta actttgtaat tattgctgtt aaaaaattaa tcacctgcca aaagaaataa
     5341 aaaagagaaa gcctccgatt aaattatttc gctacactgg ttccactttt gtgatttaca
     5401 cgggttcccc atgaagctga ccatcattcg attagaaaac tttagcgacc aggaccggat
     5461 tgacctgcaa aagatctggc cggagtattc cccttcctct ttacagggtg acgataacca
     5521 ccgtatctac gccgcgcgtt ttaacgagcg cctgctagcg gccgtgcggg tgaccttaag
     5581 cggcaccgag ggagcactgg attccctgtg cgtgcgggaa gtcacccgcc gtcgcggtgt
     5641 ggggcaatat ctgctggaag aggttttgcg taacaatcct ggcgtttcat gctggtggat
     5701 ggcggatgca ggcgtggaag atcgcggtgt gatgacggcg tttatgcagg cactgggatt
     5761 tacggcgcaa cagggcggct gggagaagcg ttaatcgctc tgtgtgaaaa gcagttcgat
     5821 ggatttggtt gggaaaaata acgcattttc gttttctccg accaacggga taaagcccag
     5881 cgattgataa aacgtatggg cttttttatt cagcgcctcg acaaaaagac cgtgaatacc
     5941 taccgctaaa gaggctgacc agacgacttt catggcatga gcaaccagtg ttgctcccca
     6001 tccctgcccc tgtaatgaac gatcaattgc cagacgccca agagtaacgc tgggaatatt
     6061 tttgtaggga atttttttct gtttcgattt cgagggcaat gcggctcgtt caaaacaact
     6121 gccgcataat gtgtaatatc ccagcacctg acgttctgga gtgttgcgac aaaggatata
     6181 cgctcgcaga attttgttgc gatgttgacg aacgagatgt gtcgtcagaa agagatttaa
     6241 cgcttcctcg ccgcagtcga atcgctgtag atcataatct gcatcatcgg tcagaatctc
     6301 tatcgtcaga tcatccataa attattacat tccctgaaga cgttttgccg cacgttttag
     6361 cttttcacca ggtgacggtg gattactcag cgcatccatc acccgcgtcc aggattcctc
     6421 attgaggatc acccgccgat gctgttcaat cacttccgca gcccgctgcg aagcgctgtt
     6481 caacataaat tggctgacgg actgattaga tatcgctgcc gcttcctcaa tcatactttt
     6541 gtcatcgtca gttaaacgca gatcgatacg ctgcttttta acagcagaca tgtgaactct
     6601 cctgctccgt ataatatgta cggtatgtca ccgtacatta agcatatcct ggattgttta
     6661 atctttctac aaccatttcg acaaacacaa aaaaacccga tggcagttca gcgcatcggg
     6721 cattttcatt tatctatctg ttacttcgca tcggttgccg tgccgttggc gtgccagtca
     6781 aatacgccga actcaaagcc tttcagatcg cctttctcat cccacgtcag cggccccatc
     6841 acggtatcaa ccgagttcgc tttcaggtat ttggcgattt cagccggatc gtcagactga
     6901 ttcaggcccg cctgcaaaga ttgcagcgcg gcgtaggtgg tccagacgaa cgcgccgctt
     6961 gggtcctgtt ttttcgcttt gatcgcgtca acaatgggtt tgttcgccgg aacctgatcg
     7021 tagttcttcg gcttggtcac cagcagccct tccgctgatt cgcccgcaat gttagacagc
     7081 gaaacgttag ccacaccttc cggccccata aactgagttt tcagccctgc cgcgcgtgcc
     7141 tgacgcagga tctgccccat ttccgggtga taaccgccgt agtaaacgaa gtcgatattc
     7201 tcttttttca gacgcgccac cagcgtagag aaatcttttt ccccggcggt gatgccatca
     7261 aagaacacca cgttagcatt gcctttcttc aggccgtcct ggaccgcacg cgccagacct
     7321 tcgccgtact gctgtttgtc gtggacgata gcaatacgct gcggtttcac cttctcaaga
     7381 atatatttcg ccgccgtcgg cccctggtcg gagtccaggc cggtggtgcg caggatcagc
     7441 tgatagccac gagcggtcag ctctggtgcg gttgccgctg gggtgatcat taaaatgcct
     7501 tcatcttcat agatgtctga cgctggctgc gttgatgaag aacagaggtg accaatcaca
     7561 tatttaatgc cgtcgttaac gactttgttc gccaccgcaa ccgcctgttt tgggtcacag
     7621 gcatcgtcat atttcacgat ttgcagtttg ttgcctttaa tgccgccttt agcgttgata
     7681 tccgcaaccg cctgctctgc gccagtaaac tcctggtcac cgtactgggc caccggaccg
     7741 gacattgcgc ccacgaccgc gactttaata tcttctgcca gagccatatt gctgaatgcc
     7801 agcgcgatac atcctgccag taacgcttta cccttcatgt tcatcctgag gttccccatt
     7861 cttctggtta ttacgtgtgt tgtgatgttg ttgttcagca ctttatttcg ttttatgcat
     7921 gactacccgt gctttagcag catactctgc taaaacatac ccgattttta tgatattgga
     7981 atagctattt tgacagttta ttaacaatct gcgtggggat tagcgttttg ccggaggggg
     8041 aaactgatta ttgcagaggc ccaaaaaaca aaaaccccgg attctcatcc agggttctct
     8101 gcttagtagt agaaattacg cttcaatggc agcgcgaagt tttttcatcg cgttcttttc
     8161 cagctggcgc acacgctcgg cggaaacacc gtaacggtca gccagttcct gcaacgtgga
     8221 cttgttgtct tcgtccagcc agcgcgcacg gatgatgtcc tggctgcgtt cgtccagacc
     8281 ctgcatcgcg tcggtcagac ggtttgccgc ctgctcttcc cagttatcat cttcgatgcc
     8341 gtcggcaaag ttagatgatt tatcctgcag atagagcacc ggtgccatcg gctggctgtc
     8401 ggaatcgtcg tcggaagaca ggtcaaaggt catgtcctgt gccgccatac gtgattccat
     8461 ctcacgaacg tctttgctgg ttacgcccag ttcacgggcc accatttcga cttcatcctg
     8521 gttaaaccag cccagacgct gcttggtttt acgcaggttg aagaacagtt tgcgctgcgc
     8581 tttggtggtc gcaactttga cgatacgcca gttacgcaga acgtattcgt ggatctctgc
     8641 tttgatccag tgaacggcga aggagaccag gcgcacaccc acttccgggt tgaaacggcg
     8701 cactgctttc atcaggccga tgttaccttc ctgaatcaaa tccgcctgtg gcaggccata
     8761 gcccgcataa ttacgagcaa tatgaacaac aaaccgcagg tgagacagga tcagcgtttt
     8821 agctgcttcc agatcgccat ggtaatgcag cttttcagcc agcgcccgct cctcgtcagc
     8881 cgacaacatc ggccacgcgt tagctgcccg gatgtaggaa tccaggttgc caactggggc
     8941 taaagctaaa ctttgcattt tgtcagtcat tcaaatcctc tcaatcgata tcttctggcg
     9001 cttcagtggt agcaacaact gtgccagagc ttaagagcaa cgaggttatc attcactgtt
     9061 ttatcagacc gtgattttat ccacaagttc aatgcaagct tgtgaataaa ttacgcacaa
     9121 aatgtgacat agagatgaaa taccgggaag agacaacggg atctctttcc ctgctacgga
     9181 acccattgca gggaaagagt ataacacgct tttattattc aggcgtaaag tggcgtaaat
     9241 gttgtaccgt ggcaagccac gctgccaccc agccaatcat cgagcatacc agcagcaata
     9301 gcaggcattc atcgaacgat aaaccattga tatcaaactt cgttccgaaa acctgtgcca
     9361 cttccgcaac cgccgatgac aatcgcagca ccagaatttc tgacaaaatt aatgacaaca
     9421 atgcgccaga aaatcccagc agtgcgccac catacaggaa cgggcgaagg atgaaaccat
     9481 ccgtcgcacc aatcagtttc tgcacgttga tggagtcacg gcgagcaaag atgctcagac
     9541 gtacgctgtt gccgatgacg aggaacactg ccgccaccat caacacgccg atcatcgccg
     9601 aaacgcgccc gaccagcccg gtcaacgccg ccagacgggc aaaccagctg tcatccatcc
     9661 gcacttcgtc aatgccgtta atctgcgtga tacgatcacg cagcgtattg agtgattccg
     9721 tcccctgaaa atcgagtttc gggatcacca ccgccactgc cggaagcggg ttttcttcca
     9781 gcatatccag cgcaccacca aaaccagacc agttacggaa ctcgcccagt gcgtcttcac
     9841 gagaaagata gttcactttc tccacgcctt gctcggcctg caactgtgcc accacgcccg
     9901 cagcggcgtc atcgtccagc gttttttgca agtaaacagt gatttgcggt gacggataat
     9961 actgcgtcgc cgcctgatta acgtttttgt acaccatata acagacgctg ggcagcgtca
    10021 gggagatggc gataaccatc accgttaaaa acgtggcgaa cggtttgctt ttcagatcct
    10081 gcaatgcgcc gtggaaggca tagcgcacct gttcgttgaa aacgttggtt ttgcgattta
    10141 ccggttttgg cgaggatttc gcgcgtttcg gtgcgttacg accgccgtcg cctgagccgc
    10201 cgaccgattt acggaagcga tccagacgcc cgccaaactg ccgaatatga ttgattgcat
    10261 cgcgcttatt catggcccac gcctccatgc aagtgaccat cgctcagggt gagcatgcga
    10321 taggaacgcc gcgaaatcag gttgatgtcg tgcgttgcca tcaatacggt taccccaacg
    10381 cggttaaact cttcaaacag acgtagaatc ccttccgaca acgcgtcgtc caggttacca
    10441 gtcggttcgt ccgccagcag taccgcgggc ttgttcacca ccgcgcgggc aatgccaaca
    10501 cgctgttgtt caccgccgga aagctgaata gggaagttct tcgctttgtc cagtagcccg
    10561 actttatcca gcgccgccga cacccggcga cgaatatcgt caccgctggc accggcgata
    10621 atcagcggga tcgccacgtt atcgtagaca gtacggtcca tcagtagatg gtgatcctgg
    10681 aaaatcatgc caatctggcg gcgcagaaac ggaacttcac ggtttttcag acgcgtgatg
    10741 tcatggccgc taaaccagat tttcccggcg ctgggccgct caatcccaca gatcagcttc
    10801 aggagggtac ttttccctgc gccggaatga ccggtcagaa acgccatctc acccggctgc
    10861 atatggaacg taacgccctg cagcgcctgt ctcccaccga gataagcctt gctgacatgt
    10921 tcaaagcgaa tcattgttaa tcctctcggg caaaaagtgc ctctataaag tcgtccgcct
    10981 taaacggacg caaatcctca atacgttcgc cgacaccaat gtagcggata gggataccaa
    11041 actggtcagc caccgagaaa attaccccgc ctttcgccgt gccgtccagt ttcgttagcg
    11101 tgatgccggt taagccaaca gcttcatgga acagtttggc ctggcttacc gcgttctgcc
    11161 cggtgctggc atcgatagtc agcataactt catgcggcgc ttcaacgtcg agtttcttca
    11221 tcacgcggac gattttcttc aactcttcca tcaggtgcga tttgttctgc aggcgtccgg
    11281 cggtatcggc aatcaggacg tcgatattac gcgctttagc ggcctgaatg gcgtcgaaga
    11341 taacagaggc ggaatccgca ccggtatgct gggcaatcac cggaatattg ttgcgctgcc
    11401 cccagacctg aagctgttca accgctgctg cacggaaagt atcgcccgcc gccagcatca
    11461 ccgatttacc ctgctgctca aactgacgcg ccagcttacc aatcgtcgtg gttttaccca
    11521 caccgttgac gcccaccatc aggatcacga acggcgtttt accttcaaca ttcagcggct
    11581 catcgacttt cgccagaatc tcgcccatct cttctttcag caggccatag agggcctcgg
    11641 catcacgaag ctgcttgcgg gatgcgcctt ccgtcagatt ggtgatgatt ttacgtgtgg
    11701 tttccacgcc cacatcggcg atcaacagct gttcttccag ctcttcaaac agatcatcgt
    11761 cgattttttt accgcggaac aggctgataa atccggaacc gagattttct ttggttttta
    11821 acaggctgcg tttcaggcgc gcgaagaaac cttctttggt cggtttttcc tgctcctgag
    11881 cgatttcttc caccggctgc tcttctaccg gaggaaccac catcaccgcc tcttctgccg
    11941 cttcggcagc cagcgcctgt gcttccagct cttcgtcggt gatttcttct ttagccgctt
    12001 cttcttccgc cgcttcgaca atctctacgg tttccgcttc agcctgccac tcttctggcg
    12061 aaacctcttc ggcgttgacg tcttccggca acggcagctc ttcacgctcg atagccactg
    12121 gctccggcgt ttcttctacg accggttccg gctgtgcaac gacttccgct tcaggctgcg
    12181 ctttttcact ttcagcaacc tgttcagtga cttccacaac gtcggcagca aaagtttccg
    12241 cctcggcttc agtatgcgcc tgcggctgct cttcgacggc ttgttcagaa gccttcacag
    12301 gctcttgcgc ctgaacgatt tcttctacaa ccggttgttc attctgaact tctgtctctt
    12361 tttccggggt ctgctctttt tgaccaaagc ccagccagga aaaaaagcca cgtttttttt
    12421 ctttcgccat ttgcgactac actcctcgct gttgcttcat ggcacagcgt taacgctatg
    12481 tacatagcag ctaaaaaaat gatgaaatag tctatcactt aacttaattc acatcaccgc
    12541 ctgcaagtat gtgttatctg gcggattgag caatttatca tgaaaaaacc gaatcattcc
    12601 ggcagcggcc aaatccgcat tattggcggg cagtggcgag gccgtaaact cccggttcct
    12661 gatagcccag gtctgcgccc caccaccgac cgcgtacgcg aaacgttgtt taactggctg
    12721 gctccggtca ttgttgacgc ccaatgtctg gattgcttcg ccgggagcgg cgcgctgggg
    12781 ctggaagcgt tatcgcgcta cgctgcgggg gccacgttga ttgagatgga tcgcgcagtt
    12841 tctcagcagt taattaagaa tctggcgaca ctaaaagcag gcaatgcacg cgtggtgaac
    12901 agcaacgcaa tgtcattcct ggcgcaaaaa ggcacaccgc ataatatcgt gtttgtcgat
    12961 ccaccgttcc gccgtggctt gttagaagag acgataaatt tactggaaga taacggctgg
    13021 ctggctgacg aagccctgat ttatgtcgaa agcgaagtcg aaaacggtct gcccactgtt
    13081 ccggcaaact ggtcattaca tcgggaaaaa gtggcgggtc aggtggctta tcggctgtat
    13141 caacgcgaag cacaaggaga aagtgatgct gattaatatt ggtcgtttgt taatgctctg
    13201 cgtttgggga tttttaatcc tcaatctggt gcatcccttc ccgcgcccgc tgaatatttt
    13261 cgttaacgtg gcgctgattt ttaccgtgct gatgcatggt atgcaactgg cgctattgaa
    13321 atccacttta ccgaaagatg gcccgcagat gactaccgcc gaaaaggtgc ggattttcct
    13381 tttcggcgtg tttgaactgc tggtctggca gaagaaattt aaagttaaaa aataacctta
    13441 ttgttcgcct acaaagctga caaagcgcgt tcctttatag ctcagcgtac ctttatcgcc
    13501 cactgtcagg gcgtggtact gctgggcgtc gaggcgaaac gtctgctcca ttcctccgct
    13561 ttgcggtttg aagcttgcct catagcgcat actggtgcct gccggagtca cttcctgctg
    13621 gcgcgaacgg cgatcgttaa tcggtttttc ccgcttgttg ctcaccacca ccagcttttg
    13681 ctggagcgga gccatatcat tatcagcttt ttcccgtcgc tgttgcataa aacgaaacga
    13741 tgcggcgacg acaattaagc caatgataac aataaagaaa agaggtggtt tgctcatctt
    13801 tatccctcat cggaaaatgc ggaaataagc ataccctgcc agttatggtg ttgtcatccg
    13861 tccaccctcg ccactaaact ggaagcaaga ccgtaggcat tccgcttacg aaaaaataac
    13921 gaattcaagg aactaagatg ctttggtcgt ttatcgctgt ctgtctttcc gcatggctat
    13981 ctgtggatgc atcgtatcgt gggccaacct ggcaacgctg ggtgtttaaa ccgttaaccc
    14041 ttcttctcct gctgttactg gcctggcaag cgccgatgtt cgacgccatt agctatctgg
    14101 tgctggcagg gctgtgcgcc tcactgctgg gcgatgcgct aaccctgttg ccacgtcaac
    14161 gtctgatgta cgccatcggc gcgtttttcc tctcgcacct gctgtacacc atctatttcg
    14221 ccagtcagat gacgctctct ttcttctggc ctctgccact ggtgttgctg gttctgggcg
    14281 cgctgttact ggcgattatc tggacgcggc tggaagagta tcgttggcct atctgcacgt
    14341 ttatcggcat gacgctggtg atggtgtggc tggcaggtga actgtggttc ttccgtccga
    14401 ccgctccggc actctctgcg tttgttggcg cttcattgct gtttatcagt aactttgtct
    14461 ggctgggtag ccactatcgc cgccgcttcc gggcagataa cgcgattgct gcggcctgct
    14521 actttgccgg tcatttcctc atcgtccgct cgctgtatct ctgataaaac ttgactctgg
    14581 agtcgactcc agagtgtatc cttcggttaa tgagaaaaaa cttaaccgga ggatgccatg
    14641 tcgactcctg acaatcacgg caagaaagcc cctcaatttg ctgcgttcaa accgctaacc
    14701 acggtacaga acgccaacga ctgttgctgc gacggcgcat gttccagctc gccaacgctc
    14761 tctgaaaacg tctccggcac ccgctatagc tggaaagtca gcggcatgga ctgcgccgcc
    14821 tgtgcgcgca aggtagaaaa tgccgtgcgc cagcttgcag gtgtgaatca ggtgcaggta
    14881 ctgttcgcca ccgaaaaact ggtggtcgat gccgacaatg acatccgtgc ccaggttgaa
    14941 tctgcggtgc aaaaagcggg ctattccctg cgcgatgagc aggccaccga cgagccacaa
    15001 gcatcgcgcc tgaaagagaa tctgccgctg attacgctaa tcgtgatgat ggcaatcagc
    15061 tggggtctgg agcaatttaa tcatccgttt gggcaactgg cgtttatcgc gaccacgctg
    15121 gttgggctgt acccgattgc tcgtcaggca ttacggctga tcaaatccgg cagctacttc
    15181 gccattgaaa ccttaatgag cgtagccgct attggtgcac tgtttattgg cgcaacggct
    15241 gaagctgcga tggtgttgct gctgtttttg attggtgaac gactggaagg ctgggccgcc
    15301 agccgcgcgc gtcagggcgt tagcgcgtta atggcgctga aaccagaaac cgccacgcgc
    15361 ctgcgtaacg gtgagcggga agaggtggcg attaacagcc tgcggcctgg cgatgtgatt
    15421 gaagtcgccg caggtgggcg tttgcctgcc gacggtaaac tgctctcacc gtttgccagt
    15481 tttgatgaaa gcgccctgac cggagaatcc attccggtgg agcgcgcgac gggcgataaa
    15541 gttcctgcag gagccaccag cgtagaccgt ctggtaacgc tggaagtgct gtcagaaccg
    15601 ggtgccagcg ccattgaccg gattctgaaa ctgatcgaag aagccgaaga gcgccgcgcc
    15661 cccattgagc ggtttatcga ccgtttcagc cgtatttata cgccagcgat tatggccgtc
    15721 gccctgctgg tgacgctggt gccaccgctg ctgtttgccg ccagctggca ggagtggatt
    15781 tataaagggc tgacgctgct gctgattggc tgcccgtgtg cgttagttat ctccacgcct
    15841 gcggcgatta cctccgggct ggcggcggca gcgcgtcgtg gggcgttgat taaaggcgga
    15901 gcggcgctgg aacagctggg tcgtgttact caggtggcgt ttgataaaac cggtacgctg
    15961 accgtcggta aaccgcgcgt taccgcgatt catccggcaa cgggtattag tgaatctgaa
    16021 ctgctgacac tggcggcggc ggtcgagcaa ggcgcgacgc atccactggc gcaggccatc
    16081 gtacgcgaag cacaggttgc tgaactcgcc attcccaccg ccgaatcaca gcgggcgctg
    16141 gtcgggtctg gcattgaagc gcaggttaac ggtgagcgcg tattgatttg cgctgccggg
    16201 aaacatcccg ctgatgcatt tgctggtttg attaatgaac tggaaagcgc cgggcaaacg
    16261 gttgtgctgg tagtacgtaa tgatgacgtg ctgggtatca ttgcattgca ggataccctg
    16321 cgcgccgacg cagcaactgc catcagtgaa ctgaacgcgc tgggggttaa aggagtgatc
    16381 ctcaccggcg ataatccacg cgcagcggcg gcaattgccg gggagctggg gctggagttt
    16441 aaagcgggcc tattgccgga agataaagtc aaagcggtga ccaagctgaa tcaacatgcg
    16501 cccctggcga tggtcggtga tggtattaac gacgcgccag cgatgaaagc tgccgccatt
    16561 gggattgcaa tgggcagtgg cacagacgtg gcgctggaaa ccgccgacgc agcattaacc
    16621 cataaccacc tgcgcggcct ggtgcaaatg attgaactgg cacgcgccac tcacgccaat
    16681 atccgccaga acatcactat tgcgctgggg ctgaaaggga tcttcctcgt caccacgctg
    16741 ctagggatga ccggactatg gctggcggtg ctggcagata ccggtgcgac cgtgctggta
    16801 acagcgaatg cgttaagatt gttgcgcagg agataaggca aaccgatcgc aacattgagc
    16861 gcgatcggtc ccctcgctcc tctggggaga gggttagggt gaggggaaaa ggcggcatcg
    16921 aagccaatct ttccccatca accgccttta cgaatcaaat aacgataagg cagtccatcc
    16981 gtctctttcg caaccagttc gtgttccata aaggtacaaa acccaggaat atcgcgggtc
    17041 gtcgccggat cgtcggcgat aatcagtaaa gtttcgccag gctgcatatt gcgcacggtt
    17101 ttgcgcacca tcatcaccgg ttccgggcag cgcaggccaa gcgcgtcgag tgtgtggtca
    17161 gggctggaaa agagatcggt cattttcttc tcatcactta aaaaaacggc gctagtttac
    17221 gccctgtgag tccgtaagca aaccaggtta acgattgcgt gaaaattaac cattgcattg
    17281 tcaacgtaaa gcagtatcat gcggcggctc gaaaaaaggg taagcacgtt attatgttaa
    17341 gataacagac gtgtcgtacg tattgggttc cctcacccca atggttaatc aaaaaggtac
    17401 aatatgaacg ttttctcgca aactcaacgc tataaggcgt tgttctggtt atcgttattt
    17461 catctgctgg tgatcacctc cagtaactat ctggtccagc ttccggtctc catttttggt
    17521 tttcatacca cctggggcgc gtttagtttt ccgtttatct ttctcgcgac cgacctgacc
    17581 gtgcgtattt ttggcgcacc gctggcccga cgcattatct tcgcggtaat gatccctgcg
    17641 ttattaatct cctatgtcat ctcgtcgctg ttctatatgg gttcatggca gggattcggc
    17701 gcactcgccc acttcaacct gtttgtcgcc cgtatcgcca ccgccagttt catggcctac
    17761 gcgctggggc aaatcctcga cgtgcacgtt tttaaccgcc tgcgtcaaag tcgccgctgg
    17821 tggctggcac cgacagcgtc cacactgttc ggtaacgtca gtgacacgct ggcctttttc
    17881 ttcattgcct tctggcgtag cccggatgcc tttatggctg aacactggat ggaaatcgcg
    17941 ctggtcgatt actgtttcaa agtgttaatc agtatcgttt tcttcctgcc aatgtatggc
    18001 gtattactca atatgctgtt gaaaagactg gcagataaat ccgaaatcaa cgctttgcag
    18061 gcgagttaaa ggttcgttat cagagttgtg ataagatgga tgaatgagcc gttatggccg
    18121 tttatcgaaa ggaagaagtc aatgcgcaat ctggttaaat atgtcggaat tggcctgctg
    18181 gttatggggc ttgcggcctg tgatgataaa gacactaacg ctacggcgca gggttcggtc
    18241 gcggaaagta acgctaccgg gaatcccgtc aacctgcttg atggcaagtt aagtttctcg
    18301 ctgccagcgg atatgaccga ccagagcggt aagctgggaa cgcaggccaa taacatgcat
    18361 gtctggtccg acgccaccgg gcagaaagca gtcatcgtca tcatgggcga tgatccgaaa
    18421 gaagatctgg cggtgctggc gaagcgtctg gaagatcagc aacgtagccg cgatccgcag
    18481 ctgcaagtgg taaccaataa agccattgag ctgaaaggtc acaaaatgca gcagttagac
    18541 agtattatct ccgcgaaagg ccagacggcg tactcttccg ttattctggg taacgtgggt
    18601 aatcaactgc tgaccatgca aattacgctg cccgctgacg atcagcaaaa agcgcagacc
    18661 accgcagaaa acatcattaa tacgctggtt attcagtaag ttttaagatg atgaggcggc
    18721 ctcagggacg tgttccggag gccgtttttt taatcgccac gtcagtaata acgcgattgc
    18781 gaccagtccc gccgccgcca gataaatcac cggtacgccc gcccagctca tcaccagccc
    18841 agccagtggt ccagtcacgc caagcgataa atccataaat acggtgtaag ttgccagcgc
    18901 cgccccctga ttttgctgcg gaaccgcttt taccgccact acacccaatg ccgggaacac
    18961 cagcgaaaac ccggcccccg ccagtaagac gccgattttc gccatccacg gcatagtcgc
    19021 cacgccaacc agtagcaggc cgattatctc aacgctaaag caaatcatcg ctacgtttaa
    19081 gccaccgata cggttaatgc cgttagggaa taacaaacgc gtaccgacaa acgcacagct
    19141 aaacagcgtc agcgcgaaag ccgcaccgtc ccaaccttta gcgtcataaa acagcgtgat
    19201 aaaggtggcg atgacgccaa atccggcgga agccagtgcc agcgccatac cgtacagcca
    19261 gacgctccca agcaccgcgc gaaacggcag cggtttgcct ttactggctt ttaccgtcgg
    19321 acgcgggatc gccaacaaaa tggccaccag cgccacgccc ataatgatta acgctaacgc
    19381 ctgcaagccg ccccagtgat aaaacacgac gcctaacggc gcacccatcg ccatcgcccc
    19441 gtaagtgaca atgccgttcc acgaaatcac ccgcccgata tgcagcgagc caaccacgcc
    19501 aacgccccac agggtcgatc ccgttccggc aaaactttgc ccaatcccaa ggatgacgcg
    19561 ccccaggcaa agtaataaca ggctgatgac aggcagactg gcggttaatc ctgccgtcag
    19621 ataccccaga ccgctcaaaa agcagccgca taaaccgaag acgacaatct ttttgggtcc
    19681 cagcaaatcg gcataacgtc cggcatgagg gcggctcagc aaggtggcga aatattgcag
    19741 gctgataacc aatcctgccc agaaagcgct aaagcccatc acatcatgga catagcccgg
    19801 taatacagcg agcggcaacc cgatggtgag gtagctggcg aagttaaaca tgacaatgga
    19861 gacaatgcgc aaattcaggc gcaatccgtt tagcgcgggt tcggctacgg gttcgggcat
    19921 gaggatcacc acatttttac aacagtgttt catttttacc atgtgctgac gtgaaaatca
    19981 gcagtaagaa tcagaatatt gctggcgtga ctcccgctac acttaataca aaaagtcaca
    20041 aggaagcccc aatggaaacc cctcaacccg ataaaacggg catgcacatt ctgctcaagc
    20101 tggcctcgct ggtagtgatc ctcgcgggca ttcacgcagc ggcagatatc attgtgcagc
    20161 tgttactggc gctgtttttt gccatcgtcc tcaaccctct cgtcacctgg tttattcgtc
    20221 ggggagtaca acgccccgtt gccattacga ttgtagtggt ggtgatgctg atcgcactaa
    20281 ccgcgctggt cggcgtactg gcggcatcgt ttaacgaatt tatctctatg ctgccgaagt
    20341 ttaataagga gctgacgcgc aaacttttta aattgcagga gatgttgcct tttcttaatt
    20401 tgcatatgtc gccggagcga atgctgcagc ggatggactc ggaaaaagtg gttaccttca
    20461 ccacagcgct aatgaccggg ctttccgggg caatggcgag cgtgcttttg ctggtgatga
    20521 ccgtagtttt tatgctgttt gaagtgcgcc acgtccctta caaaatgcgt tttgcgctga
    20581 ataatccaca gattcacatc gcgggattac accgcgcact taaaggcgtt tcgcactatc
    20641 ttgcattgaa gacgctactc agtttatgga caggtgtcat cgtctggctg gggctggagc
    20701 tgatgggggt acagtttgcg ctgatgtggg cagtactggc gtttttgctt aactacgtgc
    20761 ccaatatcgg cgcggtaatt tccgccgtac cgccaatgat tcaggtgctg ctgtttaatg
    20821 gtatttacga atgtattctg gtcggcgcat tgtttttagt tgtccatatg gtcatcggca
    20881 atattttaga gccacggatg atgggccatc gcctggggat gtccaccatg gtggtatttc
    20941 tttcattgtt aatctgggga tggctgctcg gcccggtagg gatgctactt tcggtgccat
    21001 taaccagcgt gtgtaaaatc tggatggaaa cgacgaaagg tggcagcaaa ctggcgattt
    21061 tactggggcc gggcagaccg aaaagtcggt taccgggatg aggcgacaag tgatacgata
    21121 cgcactttca ttttccatta aacgttggcc ctgatatgta tcggatagtt ctggggaaag
    21181 tttcgacctt aagcgcagct ccactgccac cgggtttacg cgagcaagca ccgcaaggtc
    21241 cacgacgcga acgctggctg gcggggcgtg cattgctttc gcacacgctt tccccgctac
    21301 cggagatcat ctatggcgaa caaggcaaac ctgcattcgc gccggaaacg ccgctatggt
    21361 tcaacttaag ccatagcggt gacgatatcg ctctgctgtt gagtgatgaa ggcgaagtcg
    21421 gctgcgatat cgaagtgatt cgcccgcgcg ccaactggcg ctggctggcg aacgccgtat
    21481 tcagcctcgg ggaacacgct gagatggacg ccgtgcatcc tgatcagcaa ctggaaatgt
    21541 tctggcgcat ctggacgcgc aaagaagcca tcgttaaaca gcgtggcggc agcgcctggc
    21601 aaatcgtcag cgtagacagc acctatcact cctcgctgtc agtcagccat tgtcagcttg
    21661 aaaatttaag cctggcgatc tgcaccccca ccccctttac gctcaccgcc gacagtgtgc
    21721 aatggatcga ttcagttaac tgatccgccc acccgactgc ccatctattg atccagaaca
    21781 ggtaatcagt atgacgaata cttaaaatcg tcatacttat ttccgccatc tattttaatc
    21841 cattggggtt accatgctct ccacactccg ccgcactcta tttgcgctgc tggcttgtgc
    21901 gtcttttatc gtccatgccg ctgcaccaga tgaaatcacc accgcctggc cggtgaatgt
    21961 cgggccacta aacccgcacc tttacacgcc taaccagatg ttcgcccaga gcatggttta
    22021 tgaaccattg gtgaaatatc aggcagacgg ttcggtgatc ccgtggctgg caaaaagctg
    22081 gactcattca gaagatggta aaacctggac cttcaccctg cgtgatgacg tgaaattctc
    22141 caacggtgaa ccgttcgatg ccgaggcggc ggcagaaaac ttccgcgcag tgctcgataa
    22201 ccgtcaacgt cacgcctggc tggagctggc aaaccagatt gttgatgtta aagcactcag
    22261 taaaacagag ctgcaaatta ccctgaaaag cgcctactat cctttcctgc aagaactggc
    22321 cctgccccgt cctttccgtt ttatcgctcc ctcgcagttt aaaaaccatg aaaccatgaa
    22381 cggaattaaa gcgccgattg gcaccggacc gtggattttg caggaatcga aactgaatca
    22441 gtacgatgtc ttcgtccgta acgaaaacta ctggggcgaa aagccagcga ttaaaaagat
    22501 cacctttaac gtcatcccgg acccgactac ccgcgcggtg gcgtttgaaa ctggcgatat
    22561 cgacctgctg tacggaaacg aagggttatt accgctcgat accttcgccc gctttagcca
    22621 gaatccggct taccacaccc aactgtcaca gccgatcgaa accgtgatgc tggcgctcaa
    22681 taccgccaaa gcccccacca acgagctggc agtacgtgaa gctcttaatt acgcggtaaa
    22741 caaaaaatcg ctgattgata acgcgttata tggcacccag caggtcgccg acaccctgtt
    22801 tgccccttct gtgccttacg ctaatctcgg cctgaaaccg cgccagtacg atccgcagaa
    22861 agcgaaagag ttgctggaaa aagccggttg gacgctgcct gcgggcaaag acatccgcga
    22921 gaaaaatggt cagccgctgc gcattgaact gtcgttcatc ggcaccgatg cgttaagcaa
    22981 atcgatggcg gaaatcattc aggctgatat gcgccagatt ggcgcagatg tctcgctgat
    23041 tggcgaagaa gagagcagta tctatgctcg tcagcgcgac ggtcgttttg gcatgatttt
    23101 ccaccgcacc tggggcgcgc catacgatcc acacgccttc ctgagttcaa tgcgcgtacc
    23161 gtcgcacgct gacttccagg cacagcaagg attagccgac aagccgctga ttgataaaga
    23221 gatcggcgaa gtgctggcga cccatgacga aacgcaacgt caggcgctgt atcgcgacat
    23281 tctgacccgt ctgcatgacg aggcggttta tctgcctatc agctacatct cgatgatggt
    23341 ggtatcaaaa ccggagctgg gtaacatccc ctacgcgccg atcgccaccg aaattccgtt
    23401 cgaacagatt aaaccggtga aaccttaatg ttgcgttacg tattacgccg ctttctgctg
    23461 ctgatcccga tggtgctcgc cgcctcggtg atcatttttc tgatgctgcg cctcggtacc
    23521 ggcgacccgg cgctcgatta tttgcgtctg tctaacctgc cgccgacgcc ggagatgctg
    23581 gcctctaccc gcaccatgct gggactggat cagccgctgt acgtccagta cggcacctgg
    23641 ttgtggaagg cgctgcatct tgactttggt atctcattcg ccagccaacg cccggtactg
    23701 gacgatatgc tgaacttcct gcccgccacg ctggaacttg caggtgcggc gctggtatta
    23761 attctgctca cttccgtacc gctcggtatc tgggcggcgc gccaccgcga ccgtctgccg
    23821 gatttcgccg tacgtttcat cgcgtttctt ggcgtgtcga tgcctaactt ctggctggcg
    23881 tttttactgg tgatggcgtt ttcggtgtat ctgcaatggc tacccgcgat gggttacggc
    23941 ggctggcagc acatcatttt gcctgcggtt tccattgcct ttatgtcgct ggcgattaac
    24001 gcgcgtttac tgcgcgccag tatgctggac gtcgccggtc agcgtcacgt cacctgggcg
    24061 cgtctgcgcg gcctgaacga caaacagacc gaacgtcgcc acatcctgcg caatgcctcg
    24121 ctgccgatga tcaccgccgt ggggatgcac atcggcgaac tgatcggcgg gacgatgatt
    24181 atcgaaaaca tctttgcctg gccgggcgtc gggcgctatg cggtgtcggc gatttttaac
    24241 cgtgactatc cggtgatcca gtgctttacg ctgatgatgg tggtggtttt tgtggtctgt
    24301 aatttgattg tcgatttgct caacgccgcg ctggacccgc gcattcgtcg tcatgaagga
    24361 gcgcacgcgt gaactttttc ctctcttccc gctggtcggt acgcctggcg ctgatcatta
    24421 tcgccctgct ggcgctgatt gcgcttacca gccagtggtg gctgccgtat gaccctcagg
    24481 ctattgatct gccgtcgcgc ctgctttcgc cggatgcgca gcactggctg ggcaccgatc
    24541 acttagggcg tgatattttc tcgcggctga tggcggcgac ccgcgtgtcg ctcggttcgg
    24601 taatggcctg cctgctgctg gtgctgacat tagggctggt gattggcggc agcgccggac
    24661 tgattggcgg acgcgttgat caggccacca tgcgcgtcgc cgatatgttt atgaccttcc
    24721 cgacctcgat tctgtcgttc tttatggttg gcgtgctcgg caccgggctg accaacgtaa
    24781 ttatcgccat cgccctgtcg cactgggcgt ggtatgcacg catggtgcgc agcctggtga
    24841 tttcactacg ccaacgcgag tttgtgctgg cgtcacggct ttccggtgcg ggccatgtgc
    24901 gggtgtttgt cgatcatctg gcaggcgcgg tgatcccttc gctgctggtg ctggcaacgc
    24961 tggatatcgg ccatatgatg ctgcacgtcg cggggatgtc tttccttggc ctcggtgtga
    25021 ccgcgccgac cgccgaatgg ggcgtgatga ttaacgacgc gcgccagtat atctggaccc
    25081 agccgctgca aatgttctgg ccggggctgg cgctgtttat cagcgtgatg gcctttaacc
    25141 tggtgggtga cgcactgcgc gatcatctgg atcctcatct ggtgacggag cacgcacact
    25201 aatgccgcaa cagattgaac tacgtaatat cgcgctacag gccgcgcagc cgctggtaca
    25261 cggtgtatcg ttaaccctgc aacgcgggcg cgtgctggcg ttagtcggcg gtagcggcag
    25321 cgggaaatca ttaacctgcg ccgcgacgct gggcattttg cccgctggcg ttcgccagac
    25381 ggcgggggaa attttagccg atggcaaacc ggtttcgcct tgcgccctgc gcggcatcaa
    25441 aattgccacc atcatgcaga acccgcgcag cgcctttaat ccactgcaca ccatgcacac
    25501 ccacgcgcgg gaaacctgcc tggcgttagg gaaacccgcc gatgacgcta cgcttaccgc
    25561 tgccatagaa gcggtggggc tggaaaacgc cgcgcgcgtg ctgaagctgt acccgttcga
    25621 gatgagcggc ggcatgttgc agcgcatgat gattgcgatg gcggtgctgt gtgaatcacc
    25681 gtttatcatc gccgatgaac cgaccaccga cctcgacgtg gtagcacagg cgcgcatcct
    25741 cgatctgctg gaaagcatta tgcaaaaaca agcgccggga atgctgctgg tcacccatga
    25801 tatgggcgtt gtggcgcgtc tggcggatga cgtggcggtg atgtcacaag gtaaaattgt
    25861 cgaacagggc gatgtagaaa cgctgtttaa cgcccccaaa catacggtga cgcgcagcct
    25921 ggtttccgct catctcgccc tctacggtat ggagctggca tcatgacctt acttaacgtc
    25981 tgcggccttt cccatcacta tgcccacggt ggatttagcg gaaaacatca acatcaggcg
    26041 gtgctgaata acgtttccct gaccctgaaa agcggcgaaa ccgtcgccct gctgggacgc
    26101 agcggctgcg gaaaaagcac cctcgcgcgg ctgctggtag gcttagagtc tccctcacaa
    26161 gggaatatta gctggcgcgg cgaaccgctg gcgaaactca atcgcgccca gcgtaaagcg
    26221 ttccgccgcg atattcagat ggtatttcag gactccatca gcgccgtgaa tccgcgcaaa
    26281 accgtgcgcg agatcctgcg tgaaccgatg cgccacctgc tatcactgaa aaaatccgaa
    26341 caactggcgc gcgccagcga aatgctgaag gcggtcgatc tcgatgacag cgttctcgac
    26401 aaacgcccac cgcagttaag cggcggccag ctccagcgcg tctgcctggc tcgcgcgctg
    26461 gcggtcgaac cgaaactact gattctggat gaagccgttt ctaaccttga tctcgtgtta
    26521 caggcgggcg tcattcgcct gctgaaaaag ctacaacaac agtttggcac cgcctgcctg
    26581 ttcatcaccc acgacttacg cctggtggaa cgcttctgtc agcgggtgat ggtgatggac
    26641 aacggacaaa tcgtcgaaac ccaggtggtg ggagataaat taaccttttc ctctgacgcc
    26701 ggacgtgtgc tacaaaacgc ggtattaccc gcattccccg tgcgccgtcg caccacagaa
    26761 aaggtttaac gcaaatgcaa cgagtcacca tcacgcttga tgacgattta ctggagacgc
    26821 tggacagcct gagccagcgt cgtggttata acaaccgttc cgaagctatc cgcgatattc
    26881 tgcgtagcgc cctggcccag gaggccactc agcagcacgg cacgcaaggt ttcgcggtgc
    26941 tctcctatgt gtatgaacac gaaaaacgcg acttagccag ccgcattgtc tccacccagc
    27001 accatcacca cgatctctcc gtcgccacgc tgcatgtgca tatcaatcac gacgactgtc
    27061 tggaaatcgc cgtgctgaaa ggtgatatgg gcgacgtgca gcattttgcc gatgacgtta
    27121 tcgcccagcg cggcgtgcgg cacgggcatt tgcagtgctt gccgaaggaa gattgagtta
    27181 tttctgaaac gaggaaagaa aagatattaa ctgaacttaa tttataaaat taagttcagt
    27241 tgcatagata gatcctgtaa aggcagatat tctgcgtaat gtacgccgct aaaatttata
    27301 aatgattcaa acgctcattc agtgtttcaa taatataggt attaacagag acttgttgct
    27361 gcgcagccgc attattaagg cgttcactca gcgattctgg atagcgtaac gtaaaggtct
    27421 taatcttttc tgtacgcgca tacggctcaa tacccgcagc tttacaatcc tcaagatact
    27481 ctcgcaggga aagttcccct tctttttgca gcccctgaat gctgtcagag acaaaatcac
    27541 aataacctga caagcccagg aatttgccac gaaatgcatt cagttctggc acgtaagtga
    27601 taacagcagg ttgcccggca atctccatag agtttggcgt cttcagtttg atcatggttt
    27661 tacccctata cttagtaacc agtcacgcac gctttctaca gcgcctttat cggtatctgg
    27721 cgacggatga ggccgatgga aacacgcaac gctcatattt agtatgaact tacagcgcga
    27781 ccccctacct tccttaattt ctccgcctaa cgctttaacc agagattcta tatcagtcca
    27841 tttgattcct tgaggtaaag gtgatttaaa tagctgatcc agtgtgtttt tttgtttctt
    27901 tcgtaaagcc tgtacctgtg tttacatgac attcccttat gacttcactt tatgacatca
    27961 cagtatgaag tcaagtttcc cttccttacg ccattgtccc aatcgtcttc ctgaatcgca
    28021 gcagcgcgat ggtgaaaaac gcgccgccaa ttgccatcag cgtcaggaac tgcggccaga
    28081 cgatttcgaa tccggcaccc cggtagagaa tggcctgcgc gaggctaaca aagtgtgtcg
    28141 tcggcatggt cagcataatg tcctgcacca tctgcggcat actttcgcgc ggcgtggatc
    28201 caccggaaag catttgcagc ggcagcagca ccagaatcac cagcagcccc agttgcggca
    28261 ttgaacgcgc tatcgtcccc ataaaaatgc cgattgacgt ggtggcaaac agactgagcg
    28321 ccacgcccag cataaacagc gggatcgagc cttcaatcgg tacacccagc acgcctttca
    28381 ccatcagcac cagtgataat cccgacacca ccagcaccac cagccccatc gaccagacct
    28441 ttgccatcat gatctcaaac ggcgttatcg gcatcaccaa caagtgttcc accgtgccgt
    28501 gttcacgctc gcggatcagc gccgatccgg tcaatacaat cgccagcatg gtaatgttgt
    28561 tgatgattgc catcaccccg ccaaaccacg cgggatcaag gttcgggtta aagcgcatcc
    28621 gggtttccag cgataccaac ggttcgctgt tatcacggta gcgcgcgaca aagctgttca
    28681 cttcaccgtt gataatattc tggatatacc cattgccggt aaatgcctgg ctcatgcgcg
    28741 tggcatcgac gttcacctga atatccggct ggcgtccggc gaggacatca cgctgaaaat
    28801 taggcggaat atttatcgca aaggtataac gcccggcgtc cagtccggca tccatctcat
    28861 cggcggtgat catctccggt ggcaaaaacc acgggcgata gaagctgtta acgatccggt
    28921 tcgataactg cgattgatcc atatcggcaa tggcgatcgg cgcgaggttc aacgatcctg
    28981 gcgtaacggt cgctgacgaa tacaccgaca ccgtaaacga gaagacaatc agcgtcagca
    29041 tcgctttatc accgagcaga ctgcgcaact ctttgatacc cagattaaaa atattgcgta
    29101 aatggcgcat catccctcct gttttttcag cagcaggata cttaagccca tcaccagcgg
    29161 gatggctatc agcaacggga taaaaagttg ccacaaatca gtcagatcca gcgctttcga
    29221 gaacgttccg cgggcgatag tcagaaaatg actggtcggg taaacctcgc cgatccaacg
    29281 tccaggccct tccagcgaag ctaccggatc gatcatcccg gaaaactgtg tcgccgggat
    29341 caacgtgata atcgccgttc cgaaaatggc ggcaatctgg cttttcataa aggtggagat
    29401 cagcagcccc attccggtgg caatgatgat atacagcagc gccgccaggg tgagcgtcag
    29461 gaaactgcct ttatgcggta cgccaaacac aaacaccgac aggccgcaga gcaggaaaaa
    29521 gttcagcatc cccagcgcga tgtatggcaa ctgtttacca agcaaaaatt cactacgcgt
    29581 ggtgggggtc acgtaaaggt tgataatcga cccaagctct ttttcccgca ccacgctaag
    29641 ggcgcttagc attgacggga tcatcatcag cagaagcggg atcaccgccg gaacaatcgc
    29701 tggcaggctt tttacgtccg ggttatagcg atagcgcgtc tcaatattca tcagcccgct
    29761 ttggctggcg ggtgtggatt gtcggctcgc cacatcctgt agccagctct ggtgcattgc
    29821 ctgcacgtaa ccttttaccg tttcggcacg gctcggcatc gctccgtcga tccagacgcc
    29881 gagttccaca ggcgtaccac gcgcgatatc gcgcccgaaa ttgggaggga tctcaattgc
    29941 caccgttata tcgcccgcac gcatccgacg atcaagctcg tcataactgg tgagcggcgg
    30001 ctgttcgata aagtaacggg aaccggagag attgagcgtc cacgcctggc tactgacggt
    30061 ctggtcgcgg tcgagcaccg caaagcgcag gttttccaca tccatactga tgccgtaacc
    30121 cattatcagc atcaggatca ctgttcccat cagcgccagc gtcgaacgta ccggatcgcg
    30181 tcgcagttcc agtgcttcgc ggcggctgta gctaaacaga cggcgcaggc taaatccctg
    30241 acgcggcgcg tgggtggtgt cgtgtatcac gggcggcgct tcggcttcgt tgctctgtcc
    30301 tgccgcttcc tgcaaatagg cgataaatgc ctcttccaga ctggcggctc cgcgtttctc
    30361 aaccagttcc tgcggtgtac cgctggcgag cacttttccg gcgtgcatca gtgagatgcg
    30421 gtcgcaacgt tccgcttcgt tcataaagtg ggtggagata aaaatggtta ctttgtcctg
    30481 gcgcgagaga tcgaccatca actgccagaa catatccctc gccaccggat cgacgccaga
    30541 agtaggttca tcgaggatta acatctccgg gcgatgaatc accgccaccg ccagcgaaag
    30601 ccgctggcga atgccgagcg gcaatgactc cggcagaaca tcttcaacgt cgttgagctt
    30661 aaaacgctcg ctcatttcag ccactcgcgc gggaatttcc gcttccggga tgtgaaacaa
    30721 acgggcatgt aactcaaggt tttgccgcac ggtgagttcg ttatagagcg aaaacgcctg
    30781 cgacatatag cccacccgac ggcgggtatc gatatctttt ggatcaaccg gttgcccaaa
    30841 cagccacgcc tcaccttcgc tggcgggcag cagtccggtg agcattttca tggtggtgga
    30901 tttaccgcag ccgttcgatc caagaaaacc aaaaatctcc ccgcgtggaa tgcggaaatt
    30961 gacgtgatca acggcaacga aggaaccaaa acgcatggtc agatcgcgcg cttcgatggc
    31021 aatctctgca ttttcaggtt gatacggtgg gatcactacc gcctgatgcg cctggcgttg
    31081 cgcttgcggt aacagattta taaacgcctc ttccagcgtg gcgctttgcg tttgctgccg
    31141 cagctcttcg gcgctgccag ttgccagcac ttctccggca ttcatcgcta ccagccagtc
    31201 gaagcgttcg gcctcttcca tataggcggt ggcgaccagc acgctcatat tgctctgccg
    31261 ctggcgaata ctgtcgatca gatcccagaa ctgggcgcgg gagagcgggt caacccccgt
    31321 tgttggctca tcaaggatca acagttccgg gtcgtggatt aacgcgcagc acagccccag
    31381 tttttgcttc atcccgccgg agagtttacc tgccggacga tcgcgaaacg gtgctaaccc
    31441 ggtgctggtc agcagctcat taattcggac ttcccgctcc gctttatcgt gaccgaacaa
    31501 gcgagcgaaa aaatcgacat tttcatacac cgataaggtg tggtagagat ttttgcccag
    31561 cccctgcggc atccaggcga tgcgcgggca gacgtcgcgg cgatgcttcg ggtcgcgcat
    31621 atcgccgccc agcaccatca cattgccctg ttcaatgacg cgggcaccgg aaatcaacga
    31681 caacaagctc gacttcccga cgccgtccgg gccaatcagc ccgaccatgc aacgggccgg
    31741 aatatcgagt gtgatattgt tcagcgcaac ggtttttcca taatgctggc tcacgcccgc
    31801 cagttgcgcg acaggcggga cgggaaccag ttccagatgc gtcattgcgg caacctcacc
    31861 acgaggtcgt caggccacgg aagttcttca ttcacccgca cccacgctac gcccggtaaa
    31921 ccggttttga catattccag atgctgctgg agtaattccg gtgggatacg cgctttgacg
    31981 cggaacatca gtttcagccg ttcatcgctg gtttcgacgg tttttggcgt gaactgggcg
    32041 acactggcga caaaactgat ggttgcagga atacgcagat ctggcgcggc atcgaggatc
    32101 agccgggctt caccgcccag tttcagcgtg cccgcctgtt cggttggcag gaagaaagtc
    32161 atatagacgt cgctgagatc gaccatattc agcacccgac cgcctgccgc cagcacttcg
    32221 cctggctcgg caacccgata ctgcacgcgt ccgtcacgcg gggctttcag ttcgctgtca
    32281 tcgatatctg cggcaatgcg ccgttcagtg gcttgtgcgg cttcgacgcg agtttgcgcc
    32341 tgaatgatat tggtgcgtgc cgcttctata gcggctttag aagccgaaac ctgagctttt
    32401 gccgattcca gcgcagctcg ggcgctctca gcggcggcgc ggtcgtcatc caactgttgc
    32461 gcagaaatag ccccacgatg ggcgagcgag cgggaacggg tatgacgttt ggctacggag
    32521 tccagttcag cttgccgttg attaaccagc gactgagcgg cgcgagtttc actttggcgt
    32581 tgctccagca aagcctgcgc ggcagcaacg gcgctttgtg cttctttgat ttgcgcgatg
    32641 gcttccagtc gctgttcctg caacacgcga gtatccatct tcgccagcac ttcaccttct
    32701 cgaacaaact ggccttcttt caccagaatg gtgtcaatac gcccggcaat tttgctggcg
    32761 atatccactt ccgtcgcttc aatgcgccca ttgctgacag caaagccttc cggcacacct
    32821 gccgggcgca acagccacca cgccacgata gccgccaccg ccagtaaccc gacaacccac
    32881 cacgccagat ggcgcttact cttatccata atcgacccgc cataatccct gtaagcaaac
    32941 gacgcgtgtt acaactgccg ccgaacgacg ttcagcggat tcttccatcc gggataagaa
    33001 ggaaacggca aatacgacca gcgcgcagag cattgagcgc caacggggaa ggcttagtga
    33061 aaacgggtag taatgttgtc atcatcagca ttcctggccg taaatgaaac acgcatactc
    33121 ataagtgtga tctataaaac tggctgacaa aacggtatcc atcctgttgg atagcgtttc
    33181 acgcctgttc agttgcagga acagagcatt cctttttacg ttttatcaac agtctcaacc
    33241 agatagtaac attaatattc atgttgtcta tggttcagag ctgtaacatt gtgcgccttc
    33301 taatcaaagg cataagtcca tttctgtttt tttcatgctg gtaagacttc cagggagtgg
    33361 ttatctcagg ccctgcacca gggttaattt ggcaattatg catgctgaac attcttccag
    33421 catgtttact gtcacgggcg ttaagaccta aaactgtccc cgtttcgcct atgcgttctc
    33481 ttccaccatt tccgttatgt gcaacaactt gatttatacc taacatttgt gaacatgcta
    33541 atagtgcttc aagtttaggt ctgtaatctg tcgaaacaga aaaataacga ttaaaggatc
    33601 gacaagagat agcccggcta gttttaaact ttttgccatt aattatagca tgatgctttt
    33661 tattgataat tgccgctaat tcgaccggat cgagagcatt ggtcatttca tcaatagaga
    33721 ctgttatcgg tcctataatg taacgttttc gctgctcatc aaaagttatc ccatgatgat
    33781 ttgccattat tttgtatttg gggtcataga aacatacagg ataatctttt atcccagcgt
    33841 aggtatcctc atcaagtggt ttcatacgcg tgaagttctg catatagtta ccattgcaat
    33901 tcgtttcatg atttccagct aatacatgaa cattttcatt gatactcatt atgcgtttta
    33961 acaaatcgat gataaattga tcgccacaga tattactaaa tcgatcgccg gtttggtcgc
    34021 caagaaacgt acatggcgtc acagcgtctt tattaataac taaataaggc tgaagacttt
    34081 ctaaaactat atctcttctg ttcagaatac tgattattgt attaacatta gtgtcaagtt
    34141 gctcattaaa gttctccctt agcaatacgt tgagaacttc atcttctata ttaagcaact
    34201 ggctaaggaa attttctcct tcagccatga ttctcaaatg tcccgttgca aaaagcgcgt
    34261 acaaaactgc accgactgag ccatcggtat cgccaaaata ggttggtctg gtacctgtgg
    34321 gatcaacagg tgaacaattg gagaaatagg ttctgtcagc gaactggggg tttatcattg
    34381 tttcgaattt aatatcagat acattgaatt gcgatatttt ccagtcccag acgggtttgt
    34441 acgactcagt aactgcaaca tcattagcat catcatttac tgtcatatta ttaagtaaat
    34501 aaacaatgct gcaccaatta atatcggtat tatcaagata gcagccgtaa tttttactat
    34561 taattgtcac aataaactga taattattat ccctacgtag aatatgagcg cgcatgtccg
    34621 atagcatact atatttttct gaatgattcg tttgtatggt aaatgatatt ttgtcatctc
    34681 gaatagattg ttggaaacgt tctatttgcc aatcatcaat tttttccaca acttcaccga
    34741 gcattgtcct ggcaatactt gatatatcat cacctatacc atttgcaata tacccaaaca
    34801 gttctttagt gaagttagta acatgctctt gcaccatatc atttgttgcg attgtcgttg
    34861 tactgtcgtt agtgcctgcc acagttccaa ttttcatctt ttacctcata taattcaccg
    34921 cgaggaatgc atagttaaca tttaatctta tgacatacac ctcatgttcg atggaaaaat
    34981 aattatattt tgcacaaatc attcagcctt aaagcataat atgaaatttc ttatctcata
    35041 gcagagaaag tccctaaaga ttgtagaggc gtcatcagaa catgcctcta caatcgatta
    35101 agaaaaccta tgaaatactg gcaggattaa aaagatatac cacagcactc atggagtttt
    35161 aatgtggttg aaaacaacta agccctggga cacctttttt atcaccacgt tcgttaggag
    35221 ctatcttcca ggaatttagc cccatggtat aattatcaat tttttgatgc tgatctaccc
    35281 ctaatccata atggccatat ttctgtttag tttttttgag cgtctgtcca catttgaaga
    35341 tatcttcagg ttttggtctg aaagcagtag tgttattaaa ataattgtca tatacaggtg
    35401 tggatgctat agcattgctt ctaattagat ggataaaacg atcatccatg tgttctttat
    35461 gttttttatt aaaaatattg gctaattcct caggatcagt aggatttttc atctgattaa
    35521 atggaacctg caaagcaccg agacaataac attttttttc ttcatctctt attattccgt
    35581 gatgactggt taaaacttgc ctttctgagt cataattaca aacatcgagc gttttaatta
    35641 agttatacgt atcaccggca gagagtttat gatttgctag tcgtgcagta tagttaccat
    35701 taaaattgat ttcatgattt ccagccagaa cgacgacatt tttattaata cggctatctt
    35761 tattaccctc catatttcgc atagaattta gcaaagtaag gatatattta tcaccaaaaa
    35821 tagtactaaa tctatcgcct gtatgatccc caaggaagat acaaggggtt atcgcatcgt
    35881 cctttaaaaa aacatgttga tgcaactcat tcaagatcat gtctctcgta tttaataaat
    35941 cgtagagcac tttattattt ttatgtttgt atgcagatga agccatctct tcgtgtttta
    36001 ataattcaca taataaactc caaccctttt ccctgatgcc tatatgccct gaaacgagca
    36061 atgcataaag tacagcgcct actcttccat ctgtatcgcc aaaataagcc gtgttggcat
    36121 tttgtgggtt aatcacacca cactgtttat agaagtctat ttttgaaaac acaggtttaa
    36181 catagctatc aaacttttcc ttagtaaacg tctctttaga gacatcccaa cgccagtcag
    36241 gtttgaaatt accactaaga agagtaaaga aatttctcgc tatgatagaa agcccatcaa
    36301 ctttataaat tatatctgat ttatctccag aggaattaat aaaataataa ccgcccttac
    36361 actcaacagt aacggtaatg ggagacgata attttaacac atggttattg tctggtataa
    36421 ttgtaaagat aaacgtatca gtagtattgt cggcgaatct attatattct tgaatatcta
    36481 tagaaggttc actattataa aaccatactt tctccatctg gtgactacga taatttacct
    36541 tccagtgacc atgttgcagg aaatagatga tatgcaggcc aaaatcgtta tctatttgcg
    36601 aatcattatc cagtttatca aattcaacta tctttgcacg atgtgtttta tcaaccttgc
    36661 catggtgttg atgactgtta actttatctg aatgcttatc ttgatggttt gaagagaagt
    36721 gcttaaatat attgtgaaat actttcataa ataacctgta atatttttac ttgttagtaa
    36781 caaaataaac aatacatgtc tgaaagacat atttacgatg atacaaagca ataatactta
    36841 tatgttcgat gattttaagc accgagacta tccggtcttt tactaaataa tggaaaacaa
    36901 tccatccctg gaagataatt gtaaaaaaaa caacagaata agaattaatc ttaggataaa
    36961 tatttattta tcatggcctt ttaaccggta aaaaatggca ctcggcacag taaaccccgc
    37021 tatagttatg ctatgcattt gtctttaatg atatctcgtc gttatattaa caggctgaaa
    37081 ttatcattaa tgcacatttc attattagca gggatacctg aggagtttaa agtgaacata
    37141 tatatcgggt ggcttttcaa attaatccct ttgattatgg gattaatttg catcgcgtta
    37201 ggtggctttg tgctggaaag ttcagggcaa agcgagtatt tcgtcgcggg tcatgtgctg
    37261 atttctctgg cggccatatg cctggcatta ttcactaccg catttattat catttcgcag
    37321 ctcacgcgcg gcgttaatac gttttacaat acattgttcc ccattattgg ctatgcgggg
    37381 tcaattatca ccatgatatg gggttgggca ctgttagcag gcaatgatgt gatggcagac
    37441 gagtttgtcg ccggccatgt tattttcggc gttggtatga ttgccgcctg tgtatcgacg
    37501 gtggcagcgt catccggtca ctttctgctc attcccaaaa atgcagcggg gagcaagagc
    37561 gatggaacgc cggtacaggc ttattcttca ttaatcggta ttgcctcatt gccgttcccg
    37621 ttttactcac cctgctcggt ttcatttggt ctattacgct gttacgtagt gctgacataa
    37681 ctccgcatta tgtcgcgggt cacgtattgc ttgggttaac cgcaatctgt gcctgtctaa
    37741 ttggccttgt tgccacaatt gtccatcaaa cacgtaatac gttttcaact aaagaacact
    37801 ggctgtggtg ttattgggtt atttttctcg gctcaatcac ggtactgcag gggatatacg
    37861 tcttagtcag ttccgatgca agcgcccgac tggctcccgg cattattctt atttgcctcg
    37921 gaatgatctg ttacagcata ttctcaaaag tctggctact ggcactggta tggagacgta
    37981 cctgttcgtt agccaacaga ataccgatga ttcccgtctt cacctgcctg ttttgccttt
    38041 tcctggcatc gtttcttgcg gaaatggcgc agaccgacat gggatatttt attccttcgc
    38101 gagttctggt cggtttggga gcggtatgct ttacgttgtt ctcaatcgtt tcaatattag
    38161 aagcgggttc tgctaaaaaa taattgcaac gtaccggata aaaccagcgt tgaccatttg
    38221 cgtaacgctg gtttttctta ggcatcatga aataacgcag cattaatgca tagtggttaa
    38281 gtataaaaaa cagcaaagtt actgtttttt tcaacctgtt catatttcat aaagatctgg
    38341 ccactgggtg agttttcaat cacctttcca tccaccttat attaagcatg gagggtttca
    38401 gttcacgggc tcattagaaa ataatcacaa gataaccact atcaaaatta gctcattttt
    38461 aatgcgactc taataatttt catctttagg aaataggtca ggacgacttt gctgcaatca
    38521 aatcctgcgc acaagcccac gcactcgacc acgcccactg gaagttatag ccccccagcc
    38581 agccggtaac gtccatcact tcgccgatga agtacagccc aggcactttg cgcgcttcca
    38641 tcgtccgtga agagagttcg ttggtgtcca cgccgccgag cgtcacttcg gcagtgcgat
    38701 agccttcagt gccgttgggt tgtacgcgcc agtcggtcaa tgtgctaatc agtgcctgtt
    38761 ggtcacgcac gttgagctgt tttagcgaaa catccgggat ttgcccgagt tgctgtaagc
    38821 gttcaaccaa ccgcttcggt agatgaaccg ccagtgtgtt tttcaggctc tgattcggat
    38881 gtgcgttacg ctgctcattc aggaaggttt cgagatccac atccggtagc agattgatac
    38941 tgacaaattc acccggttgc cagtagctgg aaatctgcaa caccgccggt ccagacaagc
    39001 cgcgatgggt gaagagtaag ttctcacgga aaacgatgcc gttttcagcg gtaatcacgg
    39061 aaggcaccgc cacgcccgcc agcacctgta actcttcgag caacggttta tgcagagtga
    39121 atggcaccag acccgcgcgg gtcggcagca cgttgaggcc aaattgttcg gcaatcttat
    39181 aaccaaacgg cgacgcgccc agccccggca ttgacagccc accggtcgcg atgaccagct
    39241 tttcgcaacc gacagtcatg ccgttcagtt caagcgtgaa gcctgtttca tccttcgcca
    39301 cactcagcac ttcgctacgc aatctgaagg ttacattgcc cttctcgcac tcatccacca
    39361 gcatgtcgac aatctgctgc gcggagtcat cgcagaagag ttgtcctaac gttttctcgt
    39421 gccaggcgat gccgtgttta ttgaccagat caatgaaatc ccactgggta aaacgcgcga
    39481 gtgcagactt acaaaaatgc ggattctggc tcagataagc gcctggttcg acataaaggt
    39541 tggtaaagtt gcagcgccca ccgccagaca taaggatttt gcgccctggt tttttaccat
    39601 tatcgatcag cagaacccgg cgtcctgcct gacctgccag cgcagaacag aacatacccg
    39661 ccgcaccagc gcctataata atggcatcaa acctttccac gttgcgctcc tcttagaaaa
    39721 aacgggcgtg aattgtaaag attcctcagt ggtcgcacca gcatcaatat tactaaaagg
    39781 aagtatttgc ctgaattata taagataatt attttttgag tgaaatccat acaggaggca
    39841 aatcaaaaaa agtctatatt tcactttgcc cgcgccgcga aagtcactga taatgcgccg
    39901 cgttcatgtc ctcaaaatgg cgtaacgtcc tatgctacat ttgtttgctg gcctggattt
    39961 gcataccggg ctgttattat tgcttgcact ggcttttgtg ctgttctacg aagccatcaa
    40021 tggtttccat gacacagcca acgccgtggc aaccgttatc tatacccgcg cgatgcgttc
    40081 tcagctcgcc gtggttatgg cggcggtgtt caactttttg ggtgttttgc tgggtggtct
    40141 gagtgttgcc tatgccattg tgcatatgct gccgacggat ctgctgctta atatgggatc
    40201 gtctcatggc cttgccatgg tgttctctat gttgctggcg gcgattatct ggaacctggg
    40261 tacctggtac tttggtttac ctgcatccag ctctcatacg ctgattggcg agatcatcgg
    40321 gattggttta accaatgcgt tgatgaccgg gacgtcagtg gtggatgcac tcaatatccc
    40381 gaaagtatta agtattttcg gttctctgat cgtttcccct attgtcggcc tggtgtttgc
    40441 tggcggtctg attttcttgc tgcgtcgcta ctggagcggc accaagaaac gcgcccgtat
    40501 ccacctgacc ccagcggagc gtgaaaagaa agacggcaag aaaaagccgc cgttctggac
    40561 gcgtatcgcg ctgatccttt ccgctatcgg cgtggcgttt tcgcacggcg cgaacgatgg
    40621 tcagaaaggc attggtctgg ttatgttggt attgattggc gtcgcgccag caggcttcgt
    40681 ggtgaacatg aatgccactg gctacgaaat cacccgtacc cgtgatgcca tcaacaacgt
    40741 cgaagcttac tttgagcagc atcctgcgct gctcaaacag gctaccggtg ctgatcagtt
    40801 agtaccggct ccggaagctg gcgcaacgca acctgcggag ttccactgcc atccgtcgaa
    40861 taccattaac gcgctcaacc gcctgaaagg tatgttgacc accgatgtgg aaagctacga
    40921 caagctgtcg cttgatcaac gtagccagat gcgccgcatt atgctgtgcg tttctgacac
    40981 tatcgacaaa gtggtgaaga tgcctggcgt gagtgctgac gatcagcgcc tgttgaagaa
    41041 actgaagtcc gacatgctta gcaccatcga gtatgcaccg gtgtggatca tcatggcggt
    41101 cgcgctggcg ttaggtatcg gtacgatgat tggctggcgc cgtgtggcaa cgactatcgg
    41161 tgagaaaatc ggtaagaaag gcatgaccta cgctcagggg atgtctgccc agatgacggc
    41221 ggcagtgtct atcggcctgg cgagttatac cgggatgccg gtttccacta ctcacgtact
    41281 ctcctcttct gtcgcgggga cgatggtggt agatggcggc ggcttacagc gtaaaaccgt
    41341 aaccagtatt ctgatggcct gggtgtttac ccttccggct gcggtactgc tttccggcgg
    41401 gctgtactgg ctctccttgc agttcctgta atcgtacgca ccaaaacgag cgggtcagtt
    41461 tactggcccg ctttttttat gcgttattat tgctgaagtg cttaatgcca aatcatcaat
    41521 gcaatcaggc tgaccaccac cagaccacac aatgcgctag tcagaataaa ctgccgacgc
    41581 acccgctcac agcggcgaat aaactcatca tcgtgatgat cgcgataacg ttgggcatag
    41641 atataccaaa cgagacgcac ctgtttgttg ggttggccat gtgaggtaaa aaagccccct
    41701 ccatcaacat attgatagag caatggatcg cagttacgca gtaccactaa caacgcgcgt
    41761 agtgatgaga aatagcgcgc catgttaaca atgcaaacga cacataaagc ccaaaataat
    41821 gcgacggtgc ttatcatacc tcctccccgg cgacctgccc gcggagttcc accccggggc
    41881 taccgctccc gatacgctgc caatcagtta acaccaggtc ctggagaaac cgcttttgtg
    41941 gtgaccaaca tccgagcggc tctatagata gtgtaggaga tcaggttgtt ttttttccag
    42001 aaggttaacc actatcaata tattcatgtc gaaaatttgt ttatctaacg agtaagcaag
    42061 gcggattgac ggatcatccg ggtcgctata aggtaaggat ggtcttaaca ctgaatcttt
    42121 acggctgggt tagccccgcg cacgtagttc gcaggacgcg ggtgacgtaa cggcacaaga
    42181 aacgctagct ggccagtcat cgacaacttt atggaaggag taacactatg gcttataaac
    42241 acattctcat cgcggtcgac ctctccccgg aaagcaaagt tctggtagag aaagcagtct
    42301 ctatggctcg cccctacaat gcgaaagttt ctctgatcca cgtagatgta aactactctg
    42361 acctatacac cgggcttatt gatgtgaatc tgggtgatat gcagaaacgc atctctgaag
    42421 agacacatca tgcgctgacc gagctttcca ctaatgcagg ctacccaatc actgaaaccc
    42481 tgagcggcag cggcgacctg ggccaggttc tggtcgatgc aatcaagaaa tacgatatgg
    42541 atctggtggt ttgtggtcac caccaggact tctggagcaa actaatgtct tccgcacgtc
    42601 agctgatcaa caccgttcac gttgatatgc tgattgttcc gctgcgcgac gaagaagaat
    42661 aatcatcccc ccacgacgtg ttcctgaacg cccgcatatg cgggcgtttt gctttttggc
    42721 gcgccttgtt acctgatgca gcgtaaaccc cttatctggc ctacgatctg cgtacgcaat
    42781 caaaatctcc tgccaataca acatttaaca ccatcatatt ttccatcatt agtgtgatca
    42841 gctggttatt ttctgttgta atagtgtatt aatctattca ccgcatcaat attaagaatc
    42901 tctgacagat gtaaactttt tcgcgcgtta tcccttacgc gttcatactt ttcaggatgg
    42961 tattggaagg ttaataaata tgaatacaac aacacccatg gggatgctgc agcaacctcg
    43021 cccatttttc atgatctttt ttgtcgagtt atgggagcga ttcggctact acggcgtgca
    43081 gggcgtactg gcggttttct tcgttaaaca gcttggattc tcgcaagagc aggcttttgt
    43141 cacttttggt gcttttgctg cgctggtcta tggcctcatt tccattggcg gctatgtcgg
    43201 cgaccacctg ctggggacca aacgcaccat tgttctcgga gcacttgtgc tggcgattgg
    43261 ctacttcatg accggcatgt cgctacttaa gcctgacctg attttcatcg ccctggggac
    43321 tatcgctgtc ggtaacggcc tgtttaaagc taacccagcc agcttgcttt cgaagtgcta
    43381 tccgccgaaa gatccgcggc ttgatggcgc attcaccctg ttctatatgt cgatcaatat
    43441 cggctcgttg atagcgttat cgctggcccc tgtgatcgct gatagattcg gttattcagt
    43501 cacctacaac ctgtgcgggg cggggttaat tatcgcatta ctggtttaca tcgcctgtcg
    43561 tggaatggtg aaagacattg gttctgaacc cgacttcaag ccgatgagct tcagcaaact
    43621 gttgtacgta ttacttggca gcgtggtgat gatcttcgta tgtgcatggc tgatgcacaa
    43681 cgtagaagtc gccaatctgg tgctgattgt tctctccatc gtcgtcacca tcatcttctt
    43741 tcgtcaggca ttcaagctgg ataaaaccgg gcgcaataaa atgtttgtcg cctttgtcct
    43801 gatgctcgaa gcggtggtgt tttacattct ctacgcccag atgccaacat cgctgaactt
    43861 ctttgccatc aacaacgtgc atcatgaaat tctcggtttt tccatcaacc cggtcagctt
    43921 ccaggcgctt aacccgttct gggtggtact cgccagccca atactggcag gcatttacac
    43981 gcatctgggt aacaaaggca aagacctctc gatgccgatg aaatttactc tcggcatgtt
    44041 tatgtgctct ctgggctttt tgacggctgc agctgcggga atgtggtttg cggatgccca
    44101 ggggctgaca tcgccatggt ttatcgtgct ggtgtactta ttccagagct taggtgaact
    44161 gtttattagc gcccttggcc tggcgatgat tgctgccctg gtgccgcagc atttgatggg
    44221 ctttattctc gggatgtggt tcctgacgca ggctgccgcg ttcttgctgg gcggctatgt
    44281 ggcaacattt accgcggtgc cggacaacat taccgatccg cttgagacgt tgcccgtcta
    44341 taccaacgtg tttggtaaga ttggtctggt cacactgggc gttgcagtag tgatgctgtt
    44401 gatggtgccg tggctgaaac gcatgattgc gacgccggaa agccattaat tattcttgca
    44461 gaaagcaggg tagcgttatc gctaccctgc tttactttta cacgggcgta cctgcataga
    44521 tatcaaagcg atgcccttta gtcacaaccg cgtttggcgt ggcgacattc gctagcggtg
    44581 gcgcgtagtc cgggcgcttt actaccacgc gtttggtcgc cagcaagcgt gcaggctcca
    44641 gcaatccatc ggcatcaaga tccggcccca ccagcgactg aaagacacgc atctcttttt
    44701 tcaccagcgc gcttttctgc ttatgcggga acatcgggtc gagatagacc acctgcgggc
    44761 gtggggtaat atcggttagc gccgtcaggc tggaggcgtg aattaactgc aaacgctcct
    44821 gcaaccagcc gccgatttcc gcatccgcat aaccacgcgc caggccgtcg tcgagcaatg
    44881 cggcaacgac tggattacgc tccagcatcc gcacgcggca accgactgaa gccagcacaa
    44941 aggcatcgcg ccccagccct gcggtggcgt ccaccacatc cggcaaataa tcgcctttaa
    45001 tgcccaccgc tttcgccacc gcctcaccgc gaccaccgcc gaatttgcgt cggtgcgcca
    45061 tcgctccgcc aacaaaatca acaaatatgc cgccaagttt tggctcatca cgcttgcgca
    45121 attccagatg ttccggcgtt aacaccagcg ccatcaggtt gtcttcatcg tgctccagcc
    45181 cccagcgggc cgccagaaca gataaggcgc cgtctccggc gcctgtttca tcaattaagc
    45241 agattttcac tgaatgatca gcccttaatg ccgtaatgct ccagcatcgc atccagctgc
    45301 ggttcacgac cacggaagcg tttgaacaga tccatcggct cttctgaacc gccacggctc
    45361 agaatgttgt cgaggaacga ctgcccggtt tcacggttga aaatgccctc ttcctcaaag
    45421 cgcgagaaag catctgccgc cagtacgtca gcccacaggt agctgtagta acctgccgca
    45481 taaccaccgg cgaaaatatg gctgaaagcg tgcgggaaac ggccccagga cggagatggc
    45541 accacggcaa ccagtttctt gatttctgcc agagtttcga ggatttttgc cccctgatcc
    45601 gggcggaact cagcatgaag gcggaaatca aacaggccga actccagctg acgcagaata
    45661 aacagcgccg cctggtagtt cttcgccgcc agcattttat ccagcaactc tttcggcagc
    45721 ggttcgccgg tttcatagtg accggagata aacgccagcg cctccggctc ccagcaccag
    45781 ttttccataa actgactcgg cagttcgacc gcatcccacg gcacaccgct gataccggaa
    45841 acaccagcgg tttcgatgcg ggtcagcata tggtgcaggc cgtgaccgaa ctcgtggaac
    45901 agggtgatca cttcgtcgtg agtgaacagc gccggtttac catttaccgg gcggttgaag
    45961 ttacaagtca aatacgcgac cggtttttgc agcgaaccat cagctttacg catctggcct
    46021 acgcagtcat ccatccacgc cccgccgcgc ttgttttcac gggcatacag atcgaggtag
    46081 aagctgccgc gcagttcgtt attttcgtca tacagttcga agaaacgtac atccggatgc
    46141 cagacatcaa catctttacg ctctttagcg gtgatgccgt aaatacgttt caccacttca
    46201 aacaggccgt taaccgcttt gttttccggg aagtacggac gcagctgttc gtcactgatg
    46261 ctgtagaggt gctgtttttg tttttcgctg tagtacgcga tatcccacgg ctgcaactca
    46321 tcgacgccaa attccgcttt ggcgaaggcg cgcagttgtg ccagctcttt ttcgccttgc
    46381 ggacgcgcgc gttttgccag atcggttaag aaatccagca cctgctgcgg gttttctgcc
    46441 attttagtag caagggattt aaaggcgtag ttttcaaagc ccagcagttg cgccagttcg
    46501 tgacgcagag cgaggatctc ttccatcacc ttgctgttat cccacttacc ggcgttcggg
    46561 ccttgatcgg aagcgcgggt gctgtaagcg cgatacatct cttcacgcaa ggcctggttg
    46621 tcgcagtagg tcattaccgg caggtagctt gggatatcca gcgtcagcag gtagccttcc
    46681 agctctttcg cttcggcctg ggcttttgcc gcagccagcg cgctttcagg catccccgcc
    46741 agctccgctt cgtcggtaac gagtttggtc cagcccatcg tcgcatcgag gacgttgttg
    46801 ctgtactggt tgcccagctc ggaaagacga gtggcaatct caccgtagcg ttgttgtttc
    46861 tctattggca gaccgatgcc agagagttcg aagtcgcgca gtgcgttatc aaccgctttt
    46921 ttctgagccg tgttcagcgt ggcgtaatga tcgccatcgc gcaggtcgcg atacgcttta
    46981 tacagccctt catgttgccc tacccaggtg ctgtattccg acagcagcgg cagggtttgt
    47041 tcgtacgctt cacgcagttc cgggctattt ttcaccgagt tcaggtggct gaccggggag
    47101 aagatacgcc ccagcacatc gtccacttcc gccaacggct ggcagagatt ttcccaggtg
    47161 tacggtgctc cttgcgctac tacgcgctcc acattttcgc ggcagtcgtt cagcgcctta
    47221 gtcacggctg gaacgacatg ttccgggaga attttagaaa acggaggcaa ttcaaaggga
    47281 gtcagtaacg gattcgtcat tcgcgcagtc ctggttaaag aggttaagga agcgctcaac
    47341 aggcgcttta cataatgtgt gtagcatggg gttaagtgta gtgaatttca atgagaaacg
    47401 ttacgctttc gcggcggcgg cctcttttcg gtatactgtc ctgataagct tttgtgcgcc
    47461 ccgaatacgg gccgattttt acttaccgga acacctttac ccatgctcag ttatcgccac
    47521 agctttcacg ctggcaacca cgccgacgtc cttaaacata ccgttcagag cctgatcatc
    47581 gagtcgctga aagagaaaga taaaccgttt ctctatctcg acacccacgc aggggccggg
    47641 cgttatcagt taggcagcga acatgccgag cgtaccggcg aatatctcga aggcatcgcc
    47701 cgtatctggc agcaggacga tttgcccgca gaactggagg cgtacatcaa tgtggtaaaa
    47761 cacttcaacc gtagcggtca gttgcgttac taccccggtt cgccattgat tgctcgccag
    47821 ctactgcgcg aacaagacag cctgcaactg accgaactgc acccgagcga ttacccgttg
    47881 ctgcgttctg aatttcagaa agacagccgt gcgcgcgtcg aaaaagccga tggtttccag
    47941 cagcttaagg ccaaactgcc gccggtttcc cgccgtggtt taatccttat cgacccgccg
    48001 tatgaaatga aaaccgacta tcaggcggtg gtcagcggga tagcagaagg ttacaaacgt
    48061 ttcgccactg gtacttacgc actgtggtat ccggtggtgc tgcgtcagca aattaagcgc
    48121 atgatccacg atctggaagc gaccggtatt cgcaaaattc tgcaaattga actggcggta
    48181 ctgccagaca gcgatcgccg tggcatgacc gcttccggca tgattgtgat taacccgccg
    48241 tggaaactgg aacagcagat gaataacgtg ctgccgtggc tgcacagcaa actggttccg
    48301 gcaggcaccg ggcacgccac cgtaagctgg atcgtgccgg agtaattgca gccattgctg
    48361 gcacctatta cgtctcgcgc tacaatcgcg gtaatcaacg ataaggacac tttgtcatga
    48421 ctaaacacta tgattacatc gccatcggcg gcggcagcgg cggtatcgcc tccatcaacc
    48481 gcgcggctat gtacggccag aaatgtgcgc tgattgaagc caaagagctg ggcggcacct
    48541 gcgtaaatgt tggctgtgtg ccgaaaaaag tgatgtggca cgcggcgcaa atccgtgaag
    48601 cgatccatat gtacggcccg gattatggtt ttgataccac tatcaataaa ttcaactggg
    48661 aaacgttgat cgccagccgt accgcctata tcgaccgtat tcatacttcc tatgaaaacg
    48721 tgctcggtaa aaataacgtt gatgtaatca aaggctttgc ccgcttcgtt gatgccaaaa
    48781 cgctggaggt aaacggcgaa accatcacgg ccgatcatat tctgatcgcc acaggcggtc
    48841 gtccgagcca cccggatatt ccgggcgtgg aatacggtat tgattctgat ggcttcttcg
    48901 cccttcctgc tttgccagag cgcgtggcgg ttgttggcgc gggttacatc gccgttgagc
    48961 tggcgggcgt gattaacggc ctcggcgcga aaacgcatct gtttgtgcgt aaacatgcgc
    49021 cgctgcgcag cttcgacccg atgatttccg aaacgctggt cgaagtgatg aacgccgaag
    49081 gcccgcagct gcacaccaac gccatcccga aagcggtagt gaaaaatgcc gatggtagcc
    49141 tgacgctgga gctggaagat ggtcgcagtg aaacggtgga ttgcctgatt tgggcgattg
    49201 gtcgcgagcc tgccaatgac aacatcaacc tggaagccgc tggcgttaaa actaacgaaa
    49261 aaggctatat cgtcgtcgat aaatatcaaa acaccaatat tgaaggtatt tacgcggtgg
    49321 gcgataacac gggtgcagtg gagctgacac cggtggcagt tgcagcgggt cgccgtctct
    49381 ctgaacgcct gtttaataac aagccggatg agcatctgga ttacagcaac attccgaccg
    49441 tggtcttcag ccatccgccg attggtactg ttggtttaac ggaaccgcag gcgcgcgagc
    49501 agtatggcga cgatcaggtg aaagtgtata aatcctcttt caccgcgatg tataccgccg
    49561 tcaccactca ccgccagccg tgccgcatga agctggtgtg cgttggatcg gaagagaaga
    49621 ttgtcggtat tcacggcatt ggctttggta tggacgaaat gttgcagggc ttcgcggtgg
    49681 cgctgaagat gggtgcaacc aaaaaagact tcgacaatac cgtcgccatt cacccaacgg
    49741 cggcagaaga gttcgtgaca atgcgttaaa tgttaaaggg ctaagagtag tgtgctctta
    49801 gcccttaatt acgtttccgc tatcagttca gaagctgaag cagaaagcgg atcagttcca
    49861 gcagcgcaat taacgcccca agaacgatga ttgctttatc aatcacccgt tttctccatg
    49921 cgatggagtg agaatgcatc cgcttactca tccactgcct gtcacggcgc atgccctatt
    49981 gttagataat ggcattctca ctccggccag agcatcagtt aacggcacca cccgtacttc
    50041 tgaccaggac tttgaaagcg tttatgcgca ttaccagagt gaaaatgcct cagagctaac
    50101 tggataatca tacagtacat tcaggttata aaaccagcat gtccttgcaa tagtttcagt
    50161 atggtattag cattgatgcg ttagataatg gcattctcac tccagccagt gccaccaact
    50221 cagggctgga aagtaaaaaa ccgacgcaaa gtcggttttt ttacatccgg attcggacaa
    50281 ggcttaatat gacgatgacc cagtgaaagt atataaatcg tcattgcgat atataccgaa
    50341 gtgccccctc cgccagctga agaaatcgct aattcttgca atgttagcca ctggctaata
    50401 gtattgagct gttagataag aactctctca ctccagccag agccaccaac tcagggctgg
    50461 aaagtaaaaa accgacgcaa agtcggtttt tttacgtcct gattcagacc tcctttcaaa
    50521 tgaatagcca actcaaaatt cacacctatt accttcctct gcacttacac attcgttaag
    50581 tcatatatgt ttttgactta tccgcttcga agagagacac tacctgcaac aatcaggagc
    50641 gcaatatgtc atttctgtta cccatccaat tgttcaaaat tcttgctgat gaaacccgtc
    50701 tgggcatcgt tttactgctc agcgaactgg gagagttatg cgtctgcgat ctctgcactg
    50761 ctctcgacca gtcgcagccc aagatctccc gccacctggc attgctgcgt gaaagcgggc
    50821 tattgctgga ccgcaagcaa ggtaagtggg ttcattaccg cttatcaccg catattccag
    50881 catgggcggc gaaaattatt gatgaggcct ggcgatgtga acaggaaaag gttcaggcga
    50941 ttgtccgcaa cctggctcga caaaactgtt ccggggacag taagaacatt tgcagttaaa
    51001 aatttagcta aacacatatg aattttcaga tgtgttttat ccgggaggca ttatgttact
    51061 ggcaggcgct atctttgtcc tgaccatcgt attggttatc tggcagccga aaggtttagg
    51121 catcggctgg agtgcaacgc tcggcgcagt actggcgtta gttacgggcg tggtccatcc
    51181 gggtgatatt ccggtggtgt ggaatatcgt ctggaacgcg acggctgcgt ttatcgccgt
    51241 cattatcatc agcctgctgc tggatgagtc cggctttttt gaatgggcgg cgctgcacgt
    51301 ctcacgctgg ggtaatggtc gtggtcgctt gctgtttacc tggattgtcc tgctcggtgc
    51361 tgccgttgcc gccctgtttg ccaatgatgg cgcggcgctt attttgacac cgattgtcat
    51421 cgccatgctg ctggctttag ggttcagtaa aggcactacg ctggcgttcg tgatggcggc
    51481 cggattcatt gccgataccg ccagcctgcc gcttattgtc tccaacctgg tgaatatcgt
    51541 ttccgctgat ttctttggcc tcggctttcg cgaatacgcc tcggtgatgg tgccggtgga
    51601 tatcgccgcg attgttgcca cgctggtgat gttacatctc tattttcgca aagatattcc
    51661 gcagaactac gatatggcgc tgctgaaatc tcccgcagaa gcgatcaaag atcctgctac
    51721 gttcaaaact ggctgggttg ttttactgct tctgctggtg ggatttttcg tcctggaacc
    51781 gctcggcatt ccggtgagcg ccattgcagc tgtgggcgcg ctgatattat ttgtcgtcgc
    51841 taaacgcggt catgcgatta atacgggtaa agtcctgcgc ggtgccccct ggcagattgt
    51901 catcttctcg ctcggcatgt atctggtggt ttatggcctg cgcaatgccg gattaacgga
    51961 atatctttct ggcgtactca acgtgctggc ggataacggc ctgtgggccg cgacgctcgg
    52021 caccggattc ctcaccgcct tcctctcttc tattatgaac aatatgccga cggtactggt
    52081 tggcgcgttg tccattgatg gcagcacggc atctggcgtt atcaaagaag cgatggttta
    52141 tgccaatgtg attggctgcg atttgggacc gaaaattacc ccgattggta gcctggcaac
    52201 gctgctctgg ctgcacgtac tttcgcagaa gaatatgact atcagctggg gatattactt
    52261 ccgtacaggg attatcatga ccctgcctgt gctgtttgtg acactcgtcg cgctggcgct
    52321 tcgtctctct ttcactttgt aatgagatac tgatatgagc aacattacca tttatcacaa
    52381 cccggcctgc ggcacatcgc gtaatacgct ggagatgatc cgcaacagcg gtacagaacc
    52441 gaccattatc cattatctgg aaacaccacc aacgcgcgat gaactggtaa aactcattgc
    52501 cgatatgggg attaccgtac gcgcgctgct gcgtaaaaac gtcgagccgt atgaggaact
    52561 gggccttgcg gaagataaat ttactgacga gcggttaatc gactttatgc ttcagcaccc
    52621 gattctgatt aaccgtccga ttgtggtgac gccgctggga actcgcctat gccgcccctc
    52681 ggaagtggtg ctggaaattc tgccagatgc gcaaaaaggc gcgttctcca aggaagatgg
    52741 cgagaaagtg gttgatgaag cgggtaagcg gttgaaataa agcggcgata tccactgccg
    52801 atagttggta aagtgcgctt tgtttatgtc ggatgcgacg ctaacgcgtc ttatccgatc
    52861 tacaaagtca tgaaaattca ataaattgta tgttatttgt agacctgaca ggcgtagcgc
    52921 atcaggcgat tttgctattt acacattact ccctgcgtga gattccaatt atcgcgtcca
    52981 gcatggtgta tcagtgatct gcttagtgat cagcgataca gtgcagcagt ttaaagacgt
    53041 actggattat gatttatcag tgctttacac aacaaattat taaataatta taagatacct
    53101 taacccattt tattattaca catctaaaac atcacaaaaa tatcatttca cacactatcg
    53161 gagaacatca taacttttta aaatgagcgc tttttgatag ttgttaacgt tttcttttac
    53221 gacactatca atgccctttt ttatacacaa atattaccgg gtggagataa aagggaagtc
    53281 aaaggtaatt atatcaggaa gatatgacgc atgatattta ttgtgcgtag aagaaaatcc
    53341 attcctgctc taattatcca atttaaaaat cttagcataa aacagataat gaataaggaa
    53401 tgttatgtca attgacttta ccccaggtat gataaataca tatcacggcg atatttataa
    53461 ccgcacaaca gataccgaca atgttaaaac accagacaca ccgatgtggc cttgtgataa
    53521 tcgggaagaa caacaaccca ttaattccac attctctgga gaaggataca accctgaaca
    53581 atatgattta gcgcggcaac acctcccaca aatttatgcg tgccgcacga acaccaaata
    53641 tacagatgca gactactcaa aagttgtggc tcaacttgta agtcttatta ctaacattga
    53701 atcgataagt tcgacaccac ttacgcggca ggcacaggaa atacttaatc aaattaataa
    53761 cattagatac gaaaagaaca aaagtgctga atgtcgaatc attgttgtcg ctaatcctaa
    53821 acccgacaag gcaatcataa ctaaaattag cgtagaagag ggaataccag taagattcag
    53881 tgtccaaacc atgttttcag atactaattt tattgctgaa caacgagctg acttaccgac
    53941 taatataaaa gatattcaaa gcctgtatca aaaaatgacg aagttatata ttgagcacag
    54001 tgaaaataaa aacaggatga aagtctttgc cggaaccaac tttattgatt tcaatatgac
    54061 aggacagaac ctgtccgggt tcgttctaac cttgtcaagg ttttatttcg aagatctact
    54121 taatataaat ttcactgacg caaatttagg tgacactatt ttttacataa agaacaccct
    54181 acccccaaat tatataaaga tggacaatat cttgacaaac aaatcgaagg tttattttca
    54241 acattattaa ccattaatga caacttttta cgagcaaaag cggaaattgc ttcgacaata
    54301 attaaatttt tagaggccag gataaccaat ctttcataca atgacatatt aacatatcag
    54361 caagaattcc aaaaacaatg ttataagcaa gttaaagcct ttacgacact ctctcgatac
    54421 aataaaattc aaacatgggc agaaatgtct gaatatcagt tcgaagtatt tcaatacgag
    54481 acgcttaacc ccaaaaaaat gtcacacacg ccttatttaa agaggccact tcctaatgaa
    54541 aaagatataa attatggggt ggaaatagaa ataccatctg gcaaaagaat tcgcttatca
    54601 aatcattatc agaatataat accataatat tgattattag cactttataa ttattgaata
    54661 aaaatagatt ttatgtaatt ttaaaacaaa ggattatatt atggggcgat ggatactcat
    54721 gccacgcccc aaaatcaatg gaattgaact aaacaaaagg gatataaaca tcagacaggt
    54781 ttacgttact atcaggcata tcacctcaga atcagatgaa aactataaag aaatatctat
    54841 tatggcttta atatttgttg ataaggatag taacatgaac atgacaaaag gtgcactcat
    54901 cctcagcctt tcatttttgc ttgccgcatg tagttcaatt ccgcaaaata tcaaaggcaa
    54961 taaccaacct gatattcaaa aaagttttgt tgctgttcat aaccagccgg ggttatatgt
    55021 tggtcaacaa gcgcgctttg gtgggaaggt tatcaacgtt atcaatggca aaacggatac
    55081 gttgttagaa atcgctgtat taccgttgga tagctatgcg aagcctgata ttgaagccaa
    55141 ctatcagggc cgactgctcg ccagacaaag cggcttcctt gatccagtga actatcgtaa
    55201 tcactttgtt accatcctcg gtaccattca gggtgaacaa ccaggcttta tcaataaagt
    55261 cccgtataac ttcctggaag tgaatatgca gggcatccag gtgtggcatt tgagagaagt
    55321 ggttaatacc acctataacc tgtgggatta cggctatggt gcattctggc cggaaccggg
    55381 ctggggtgcg ccttactaca ccaatgcggt gagtcaggta acacctgagc tggtcaaata
    55441 acaccaccga aagatgcaga tgctttctca gcatctgcat catgtattac ataaaattaa
    55501 tatacagtaa gctaactatt attattataa gccctgtcct gttaattacc tttggcaaac
    55561 tgattataaa gttaatgtcc gcaccaggag tcggttatgt ttcttataat taccagggat
    55621 acgatgttct tcaccgcgat gaaaaacatt ctgagtaaag gtaatgtcgt tcatatacag
    55681 aacgaagaag agatcgacgt aatgttgcat cagaatgcct tcgtcattat tgatacatta
    55741 atgaataatg tatttcattc taattttctc actcaaattg aacgattaaa acctgtccat
    55801 gtgattattt tctccccctt taatattaaa cgctgtctgg ggaaagtacc ggtgaccttt
    55861 gttccgcgga ctatcactat cattgatttt gtcgcactca tcaatggcag ttactgctct
    55921 gtgcctgaag cgaatgtgtc actttcgcgc aagcaacatc aggttctgag ctgcattgcg
    55981 aatcaaatga caacggaaga tattctggag aaactgaaaa tatcgctaaa aacgttctac
    56041 tgccataaac acaatatcat gatgatcctc aatcttaagc ggatcaatga gctggtacgc
    56101 catcagcata ttgattatct ggtgtgaatt tcaggcttac ggtgagtctg gctacgctgc
    56161 cacacagatt agctaattga aacgcctttc acccctgcca taccttttaa taatcgcaac
    56221 aggtcttcta tcgacgttgt ggcatgaagc tggatatcaa tggcgaccac ttcatgatct
    56281 tcattttcct gcaacgaaac taaatccgtt tttatttttt gctgcttaaa ccagtcgagc
    56341 attgataccg tattaccgtt cactaatgtc agctgtaaat ggtaattttt gttcatcagg
    56401 cggaaggtta attgatggaa gacttccagc accaacaagg tcatcactga accataaata
    56461 cccagttcgt acataccgct gccaataacc atacctatgg cggcggtcac ccagatatcc
    56521 gctgccgtcg tcagacctac aatattttgg ttacgaacga gaatgttacc tgcaccgata
    56581 aacccgacgc ccgtcaccac ctgagcagca atacggctgg ggtcgagtcc gacgtgatct
    56641 aaagacagca cgtcagcaaa accatatttc gaaacaatca taaacagggc gcttcccatg
    56701 ccaattaata catgtgtgcg taaccctgct cctttgccgc gcatttgcct ttccatgcca
    56761 atagcgccac aggcaattgc cgcgagtata agacgtataa taaattctgc tgtcatataa
    56821 aaatgcaaaa aggagcagca agatggctca acttgctact cctttttact tgtacctcat
    56881 taattcggca agtcattaga tgcttgattt ttgaaggtat acaaattttt ctgcgggttt
    56941 ttcttacagt attcgatcac tttaggaatt tgagtgagat cggtttcatt taaagtaacg
    57001 gtatcgccac ctttatatac tgtttcttca tgcagcatcc accatgcaac cggggtcatt
    57061 gcttttggat tcagatcaat aaattcctgg caggtcatat ctttagcgga ttcattggct
    57121 gccagcgcag attgtgcgtt caccagtgac aaagccgcta cagcgcccat aaaaataaac
    57181 gctttacgga gagatgaaat attcattttg taacccattc aatatagaga ttatatatga
    57241 acaggaggaa tagctgcttt taaagaagat attccggcct gttcaatatg caaggaagta
    57301 cgatgtaacg cattcccgga attacatatc tttcttaatt ttgtcccatt cgcctttaac
    57361 tttatcttta aagttggctt gtttatcctg agtacaagcc tgaacgatag ctggggttac
    57421 ggttgcaata ccctgaacat ctaaaaccgc atcttctggt ttatctttgt tgttcagcgc
    57481 ttcagcaaaa ccaactgcag ttggctggaa ggattcgtcc acagccagga aatcttcaca
    57541 ggtccaggag ttgaccggtt ttttgttatc agctgctttt tgcgcatccg ctgcattgct
    57601 tacaactggc agaagaagca gaccaccaag aataacgcct aatacttttt tcatcgtaat
    57661 atcctcaact ataaagtgaa agagccgtca cgaatcaatt tcgacactga ggttataacc
    57721 tggttttctg tatatgtcat gttgatagaa aatatcaaaa tcagatattt ttatttcaat
    57781 acaatgagtt acagatgcat cagatactgt aattaggaaa tttttattaa atcgactgca
    57841 ttcttagacg cgtttttggc atagattgat agcaggggat tttcttctta attttatagg
    57901 gtggttctat gttatatata gataaggcaa caattttgaa gtttgatctg gagatgctta
    57961 aaaaacatcg cagagcaatc cagtttattg ccgtgctgtt gtttatcgtc gggttgctgt
    58021 gtatcagttt cccgttcgtc tctggcgata ttttaagcac agtagtgggt gcattattaa
    58081 tctgctcggg cattgcgctt attgtcgggt tattcagcaa ccgcagtcat aatttctggc
    58141 cggtattatc cggtttcctc gtcgcagtcg cctatttatt gatcggctat ttcttcatcc
    58201 gcgcaccgga gctgggcatt tttgccattg cggcatttat tgccggtttg ttctgtgttg
    58261 caggggttat tcgcctgatg agttggtatc gtcagcgttc aatgaaaggc agctggctac
    58321 agctcgttat tggtgtgctg gatatcgtca ttgcctggat attccttggt gcaacgccga
    58381 tggtgtctgt aacgctggtt tccacactgg tgggaattga actgatattt agcgccgcca
    58441 gcctgttcag cttcgccagt ttgttcgtta agcagcaata attatcccgg ttgtcacccg
    58501 gatcatagtc acttgatgtg actatgatcc gattaatact ctcaccgcta cgcagcgttg
    58561 tagatcaatt gcgcactatc attgaaataa ttacctgcta gtgattattt caacctactg
    58621 aatttcatct aatttttttc actccatggc aaattagcca tttcaaacat tatcatggct
    58681 gatattttcc gtagtcaggt ttaatgtttt aaaagtgcag tgggaaagtg aacaaagagt
    58741 tccgtaagcg ttgatgctat gggcggttaa ataagtaatc cgggttcatt tttttgcaac
    58801 tggcattgat tacattgcat aaatatccgt gtctccagac gctatataaa aacctgaaga
    58861 catgaatgcg ttatttactc aggtaatttc aatgcgttaa aagaaagctg gcaatccaat
    58921 tgccagctta agtcgaaaca aggagactcg atatttaaat cggattacat tttaacttta
    58981 gtaatattct tcagagatca caaactggtt attgataact tattcttggg cagtaatccg
    59041 caaacgttaa ctttttgttt actgtttaca aactgataac aaccaggaat cttacttagg
    59101 atcaatatat ggagtgcgtg atggataaat ctgaagtatt gattagtgtt aatagacgta
    59161 ttagttcacg aagggcaaag ttcttatagg cgtttactat attgaacaac gattcggaca
    59221 aggatgtaaa taatgaaaag gatgacatgt tcgaaacgat aacggctaag gagcaagtta
    59281 tgatttttct catgacgaaa gattcttttc ttttacaggg cttttggcag ttgaaagata
    59341 atcacgaaat gataaaaatc aattccctgt cagagatcaa aaaagtaggc aataaaccct
    59401 tcaaggttat cattgatacc tatcacaatc atatccttga tgaagaagcg attaaatttc
    59461 tggagaaatt agatgccgag agaattattg ttttggcacc ttatcacatc agtaaactaa
    59521 aagctaaagc gcctatttat tttgttagcc gcaaagaaag tatcaaaaat cttcttgaga
    59581 ttacttatgg taaacacttg ccccataaga attcacaatt atgtttttca cataatcagt
    59641 tcaaaattat gcaactgatt ctgaaaaata aaaatgaaag caatatcacg tcgacgctca
    59701 atatttcgca acaaacatta aagattcaga aattcaacat tatgtacaag ctgaaactaa
    59761 gacgtatgag cgacatcgtc accctgggta tcacatctta tttttagtca ggacataagc
    59821 aactgaaatt gatggctggc atgacgaggg atgcagatgc tgatttcatt acccccggtg
    59881 attactaaag gagaggctaa aacgacttta ttcccctggt atgtgtatcc accagtagaa
    59941 cccttcgttg cccgaatgct ggcaggaact gttggcagaa cggcaacatt ttttttgtcg
    60001 ttgacctcac catgtcgatc actatacctg tatcccacct tactggctga caaccccact
    60061 atgccgctgg tctgtaaatc cctcatatct ctcctcgcgc gcaatttaaa gaaccgttat
    60121 ttctcaagaa ttttcaggga ctaaaatgaa cagaagaaga aagctgttaa taccgttgtt
    60181 attctgcggc gcgatgctca ccgcctgcga tgacaaatcg gcggaaaacg ccgccgccat
    60241 gacgcctgag gtcggtgtcg tcacactctc ccccggttcg gtcaatgtgt tgagcgaatt
    60301 gcccggtaga accgttcctt atgaagttgc cgagatacgt ccccaggtgg gcggtattat
    60361 cattaaacgc aactttatcg aaggcgataa agtgaaccaa ggcgattcgc tgtatcagat
    60421 tgatcctgca cctttacagg ccgagctaaa ctccgccaaa ggctcgctgg cgaaagcgct
    60481 ctctaccgcc agcaatgccc gcatcacctt taaccgccag gcatcgttgc tgaagaccaa
    60541 ctacgttagc cgtcaggatt acgacaccgc gcgcacccag ttgaatgaag cagaagccaa
    60601 tgtcaccgtc gccaaagcgg ctgttgaaca ggcgacgatc aatctgcaat acgcgaatgt
    60661 cacctcgccg attacgggcg tcagcgggaa atcgtcggtg accgtcggcg cactcgttac
    60721 cgctaatcag gcagattcgc tggttaccgt acaacgtctg gacccgattt atgtcgatct
    60781 cacgcagtcg gtgcaagatt tcttacgcat gaaagaagag gtcgccagtg ggcaaatcaa
    60841 acaggttcag ggcagtacgc cagtacagct caatctggaa aatggtaaac gctacagcca
    60901 gaccggcacg ctgaaattct ccgacccgac ggtggatgaa accacgggct ccgtgacgtt
    60961 acgggcgatt ttccccaacc caaatggtga cttgctgcct ggcatgtacg tcacggcatt
    61021 agtggatgaa ggtagccgcc agaatgtatt actggtgccg caggaaggcg tcacccacaa
    61081 cgcccagggt aaagcaacgg cgctcattct ggataaagac gatgttgtgc agctacgcga
    61141 aattgaagcc agcaaagcca tcggcgacca gtgggttgtc acctctggct tgcaggctgg
    61201 cgatcgggtg atcgtttccg gtttgcaacg cattcgtccg ggtatcaaag cacgagcaat
    61261 ttcctccagc caggaaaacg ccagcaccga atcgaaacaa taacgttgca ggcttaaggg
    61321 gactttcatg gctaactatt ttattgatcg cccggttttt gcctgggtac ttgccattat
    61381 tatgatgctt gcaggtggtc tggcgatcat gaacttaccg gttgcgcagt atccgcagat
    61441 tgcgccaccg accattaccg tcagcgctac ctatccaggt gccgatgcgc aaacggtaga
    61501 agactcggtc actcaggtga ttgagcaaaa tatgaatggg cttgatggcc tgatgtacat
    61561 gtcttcaacc agtgatgcgg cgggtaatgc ctctatcact ctgaccttcg agactgggac
    61621 atctcctgat atcgcacagg ttcaggtgca aaataaactg caactcgcta tgccttcatt
    61681 acctgaagcg gtgcagcagc aggggattag cgttgataag tcgagcagta atattctgat
    61741 ggtagcggcg tttatttctg ataacggcag cctcaaccag tacgatatcg cggactatgt
    61801 agcgtctaat atcaaagacc cgctaagccg taccgcgggc gttggtagcg tacagctctt
    61861 tggttccgag tacgccatgc gtatctggct ggacccgcaa aaactcaata aatacaatct
    61921 ggtaccttcc gatgttattt cccagattaa ggtgcaaaac aaccagattt ccggtggtca
    61981 actgggtggc atgccacagg cggcagacca gcaactaaac gcctcgatca ttgtgcagac
    62041 gcgtctgcaa acgccggaag aatttggcaa aatcctgttg aaagttcagc aagatggttc
    62101 gcaagtgctg ctgcgtgatg tcgcccgcgt cgaacttgga gcggaagatt attccaccgt
    62161 ggcgcgctat aacggcaaac ctgctgccgg gatcgccatc aaactggcta ccggagcaaa
    62221 cgccctggat acctcgcggg cagtcaaaga ggagctgaac cgcttatcag cctatttccc
    62281 cgcaagtctg aagacggttt atccttacga caccacgccg tttatcgaaa tttctattca
    62341 ggaagttttt aaaacactgg ttgaggctat catcctcgtc ttcctggtca tgtatctgtt
    62401 tttgcagaat ttccgtgcca caatcatccc gacgattgcc gtaccggtgg ttattctcgg
    62461 gacgtttgcg atcttgtcgg cggtcggttt caccatcaac acgttaacta tgttcgggat
    62521 ggtgctggcg atagggctac tggtggatga cgccatcgtg gtggtggaga acgtcgagcg
    62581 tgtcattgcg gaagataagc taccaccgaa ggaagcgacg cataaatcga tggggcagat
    62641 ccaacgtgcg ctggtcggta ttgctgttgt tctttccgca gtgtttatgc cgatggcctt
    62701 tatgagcggc gcaaccgggg agatctaccg ccagttctcc atcacgctga tctcctccat
    62761 gctgctttca gtatttgtgg caatgagcct gacccctgcc ctgtgcgcca ccattctgaa
    62821 agccgcgccg gaaggcggtc acaaacctaa cgccctgttc gcacgcttca acacgctgtt
    62881 tgaaaaatca actcagcact ataccgatag cacccgctcg ctgttgcgtt gtaccggtcg
    62941 ctacatggtg gtctacctgc tgatttgcgc cgggatggcg gtgctgttcc tgcgtacacc
    63001 gacctctttc ttaccagaag aggatcaggg ggtatttatg accaccgcgc agttaccttc
    63061 cggtgccacc atggttaaca ccacgaaagt gctgcaacag gtgacggatt attatcttat
    63121 aaaagagaaa gataatgttc agtcggtgtt taccgttggc ggctttggct tcagcggcca
    63181 ggggcaaaac aacggcctgg cgtttatcag tctcaagccg tggtctgaac gtgtcggtga
    63241 ggaaaactcg gttaccgcga tcattcagcg ggcaatgatt gcgttaagca gtatcaataa
    63301 agccgtcgtc ttcccgttca acttacccgc ggtggctgaa ctgggtaccg cgtcaggttt
    63361 tgatatggaa ctgctggaca acggtaacct ggggcacgaa aaactaaccc aggcacgaaa
    63421 cgagctgtta tcactggcag cgcaatcgcc tgaccaggtc accggggtac gcccgaacgg
    63481 cctggaagat acgccgatgt tcaaagtgaa cgtcaacgcc gcgaaagctg aggcaatggg
    63541 tgtggcgctg tctgatatca accagacaat ttccaccgcc ttcggcagca gctacgtgaa
    63601 cgacttcctc aaccaggggc gggtgaaaaa agtgtatgtc caggcaggca cgccgttccg
    63661 tatgttgccg gataacatca accaatggta tgtacgcaac gcctctggca cgatggcacc
    63721 gctttctgcc tactcgtcta ccgaatggac ctatggttca ccgcgattgg aacgctacaa
    63781 cggcatcccg tcaatggaga ttttaggtga agcggcggcc gggaaaagta ccggtgacgc
    63841 tatgaaattt atggcagacc tggtcgctaa acttccggca ggcgtcggct actcatggac
    63901 cggactgtcg tatcaggaag cgttatcctc aaaccaggct cctgcactgt atgccatttc
    63961 actggtcgtg gtgttcctcg ccctcgccgc actctatgag agctggtcaa ttccgttctc
    64021 ggtgatgttg gttgttccgt taggcgtcgt tggcgcatta ctggccaccg atctgcgcgg
    64081 cttaagtaat gacgtctact tccaggttgg tttgctgacc accatcgggc tttccgccaa
    64141 gaacgccatc ctgattgtcg aatttgccgt tgagatgatg cagaaagaag ggaaaacgcc
    64201 gatagaggca atcatcgaag cggcgcggat gcgtttacgc ccaatcctga tgacctctct
    64261 ggcctttatt ctcggcgtgc tgccgctggt tatcagtcat ggtgccggtt ctggcgcgca
    64321 aaacgcggta ggtaccggcg tgatgggcgg gatgtttgcc gcaacagtgc tggcaattta
    64381 cttcgttccg gtctttttcg ttgtagtgga acatctcttt gcccgcttta aaaaagcgta
    64441 acgtgtaaat gagagtaagg ttgaacgtga aggttcagcc ttactctttc ctgctaacca
    64501 ttcaccactc aacaaccagc taaccgtcag gaataccagg cttagtacac agcactgaaa
    64561 gtagaaacca ctccagccat tcgtcatcaa tttgaacagc acaatactga cccacattcc
    64621 cgtaatcgta tgaattgtca gcttaataat cccaaacatg ccgagtcttt tcctcctgaa
    64681 aatacctata ccgcctggca aactgcgaaa gagaactagc cggtagcccg gcagaaatca
    64741 tcagggaaga gtttcacatg aagcaggtgt gagatcctga ccaatattca aatgcaaaat
    64801 atgtcaggaa aaggtacctg gcgaatgttg cgcaaactga tgtggcgtta caccataata
    64861 ttgtcgaaat gtgtttataa agtacgacgt actgctatag ccacattttt ctgcaatagt
    64921 atgcagagga atttgacgta actcgagtaa tcgtctggcc atcgacatcc tggaagcgag
    64981 taatatttta ctgaaacagg tattttcatc ctgcaacttt tttttgatga gactctcgct
    65041 ggtatacatt ctttccgcga tatcgcgcag ataccaacgt ttggcgatat caaagctaat
    65101 aaggcgttca acttttcctg aaacagtgct gatgctattg aaaagtaagg ggatcagttc
    65161 tttcttatga gaaaacatcg ataaacagga aaggtagagc atattgctca gtatcggtga
    65221 atttaccccg gattcattga agctatcaaa aatcgccgtc accagcggca tggggggtga
    65281 ctggttaatt aatagacggt ctttgctatg taaccctaaa ttgcgtggta gctgacgaat
    65341 attttgtaag taatggcaaa ctgtcagctc atcgatatcc agtcgtcgcg tatccggtga
    65401 acaaaacgca aaatcgtctg ccaaattttt ctccagcagc acgatactct cgttatgcag
    65461 cgatatttta tgctgttcat gataaatatc gaatgaacga cgaatgagga tcaccgagca
    65521 gacatgagtc atgattatcc cttatatttc atactgcgat tatttcaatt tcagtatact
    65581 aatgaaatgt tgccagactg tttcttagcg aaaaaactgc cagatttggt aggaccaaaa
    65641 tttatccaca aagtagtttt gcataacagt taattgataa taagtaaatg cagcacgaat
    65701 atattttcgc actgcgtata gcttatgttt ataaaaaaat ggctgatctt atttccagta
    65761 aaagttatat ttaacttact gagagcacaa agtttcccgt gccaacaggg agtgttataa
    65821 cggtttatta gtctggagac ggcagactat cctcttcccg gtcccctatg ccgggttttt
    65881 tttatgtctg agtaaaattc tataatctta ttccttccgc agaacggtca gtgccgtaaa
    65941 aatttccttg cgcaacaata cttgccgccg agtcacggtt cggcaatccc tgcgccgatc
    66001 gctcctgata ctctgtgggc gtcatcccat aataatttcg aaagacgtaa atgaaatacg
    66061 acacgctgtg atatccacag gacactgcga ctcgcttaat tgaaaagcca tgtataacaa
    66121 taagttgcaa agcacgttgc attctacact cagtaagcaa ctgtgaatat gatgtctctt
    66181 cttcgcgcaa ttttttcttt aacaggcttg gactcatcaa cagctcgctg gcgattcggg
    66241 ctagtgtcca ctcatgggcg atattattat tgataaccgt acaaactcgt gttcgcatgt
    66301 tcggttgcaa aacgttcaaa agcagcggta taaagtgctc atcctcaaga aaaacagata
    66361 acaacgcaaa gattaatgct cttttacgta gcatttctgc atagcgaaga ttcttattct
    66421 gcgatagttg cgcaacttcc tgaaaaacag gaatatctct gctacagtga tgaacgagta
    66481 atgaagaaat ttttttatgt gcatgaagat ttaatgcctc ctccttgagc atcggcagaa
    66541 ataatgaaag cgtatccctt gatactaata caaaattttc aacacactta tctactcgaa
    66601 tttggcttgc atccgcaaaa accaggtcac cgccattaaa atagcgatat tcaccattaa
    66661 ccatggtgag aatatattta tgtcttgcgt acgcaattag acaattccca tgtaatgatt
    66721 gcatagttga cttaatatta cataaacata ttactgttaa ttaatgtagc acgccaccct
    66781 cttaagtcaa attgattgat aaataaattt aaacgaagcg ggagaacagg atgataatgt
    66841 gtgatgttct acgggcagga tgactggatt tataatacaa aaacgtgttt aagaacgcag
    66901 tgaaattaat agcaagcaaa taatcccttt tcgtgacatt aaaggcaatc gctacattta
    66961 ataaacattc atataacata tatcttatca acacgatgaa tagacagcca atatattatt
    67021 gcgattaata agcaaccgaa tgcccagctg tttttttaaa ggctgggcat tcggttttta
    67081 caacgttatg ttatcaggtg tgtttaaagc tgttctgctg ggcaataccc tgcagtttcg
    67141 ggtgatcgct gagatatttc agggaggctt tgtagtcttc cagcaacagt tcagcaaagt
    67201 ccatttcgaa gccgcgacga cacataatgc gcatcaccac gatgtcggtg gcttcaccgc
    67261 cgagagtgaa ggccggaacc tgccagccgc gcagacgcag acgttcagag aggtcgtaca
    67321 gggtgtatcc cggatcttca ccatctttca gtttgaagca aaccgccggg atgccttcgt
    67381 ccgggcgacc cgtacagatg aactcatacg gccccagttt ggcgatttca tccgccagat
    67441 aagcggcaac ctggtaagag gcgttctgta ctttggtata gccttcacga ccg
//
LOCUS       DAMZET010000009        58441 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.009, whole genome shotgun sequence.
ACCESSION   DAMZET010000009 DAMZET010000000
VERSION     DAMZET010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.009"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(657..809)
                     /locus_tag="POB34_000690"
     CDS             complement(657..809)
                     /locus_tag="POB34_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HDI5917686.1"
                     /translation="MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATL
                     AYEVKR"
     gene            1101..2309
                     /gene="trbA"
                     /locus_tag="POB34_000691"
     CDS             1101..2309
                     /gene="trbA"
                     /locus_tag="POB34_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HDI5917687.1"
                     /translation="MSYNRQPVAEDPMQIWGAVGVLLILLLFVIWLFLPEVVYASCLI
                     LHTLWGLVDWGPFHNYAAPRYNLLAMTGNNAANISYSQWVNVMEQTIGILWMYLLPVT
                     LWCLWEWYQHPGQSRFTRRPVDITRLPYIFASLSPAIAPVLADGDPEKLFHGGKRPER
                     RVALTPEAFVEQHTLITNMQLDVAAARRCFMAQLGKPLTSWKDMAPHEKALFAIFGLQ
                     YFLDDRKAALKLMDTLNLSCRIKSKRDSGKFCTPVYSLAKSAFQRVIKSNGAQQWLKQ
                     HRYVRSGLVWLYAHDLRLTPPNWIWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     EAEAMRFGLPCPEPCVDEAVEGLRRDMLSLGLIWDEPQPDRDRKRRILTNWSLTDDIL
                     PRTPATDNEF"
     gene            2328..3398
                     /gene="trbB"
                     /locus_tag="POB34_000692"
     CDS             2328..3398
                     /gene="trbB"
                     /locus_tag="POB34_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbB"
                     /protein_id="HDI5917688.1"
                     /translation="MTIEYFATRIAKHISVQAIWPDGRTEIIAVLPRDALSGLVTRNN
                     QLIHIAFDAMGTRNRETVLIDKQEHNADQILTAIDSCLRLEYLMERRFSRTPLFRGII
                     ASVVLFVMATIGFSLFRYVDRVFWDDTTPEAVQTAGEPRLLPPHLNHTVPLNEGIQLP
                     VPPKKDVQVPEKTITSKNPEAAAARHNLATVLKRNADRGMFTINLSSGHERTLFAFLD
                     PACPNCRLLEPALKRLASDFNVVIYPVSVIGGEESTDRVAPLLCEKDAQKRAAGWHRL
                     YSADNGMMTPSEETTPADETCLKAARAAIDVNNVAFRKFGFAGTPWVLSDTGWHLPTG
                     ILQETGTLNLFLKTTDSESGHE"
     gene            3391..5682
                     /locus_tag="POB34_000693"
     CDS             3391..5682
                     /locus_tag="POB34_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HDI5917689.1"
                     /translation="MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIW
                     PLALPACLLFTLITMLVFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQY
                     EVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINP
                     LCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTR
                     PQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTS
                     AHTTSALGRMFITQKSMILARDLGYRLEGTDSDALEVKKYKGRFPYLCFLDEVGAYYT
                     DRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLMQNTGTKFAGRIVSEGSTA
                     RTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELHPGEFFS
                     IFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPE
                     NVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESA
                     LWETAVNTLKTTTREERRIRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNH
                     PTGRPVNGFHHKKTNRPDWDGMY"
     gene            complement(5719..8418)
                     /gene="nikB"
                     /locus_tag="POB34_000694"
     CDS             complement(5719..8418)
                     /gene="nikB"
                     /locus_tag="POB34_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxase NikB"
                     /protein_id="HDI5917690.1"
                     /translation="MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQ
                     PHRSRFSRLVDYATRLRNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEY
                     IARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVRHTLKSLGLADHQYVSAVHTD
                     TDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRI
                     VRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQ
                     MDGKFLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNP
                     DFIAADVRPEKIAETESLQQYACRHLGERLPEMAREGRLENCLAIHRTLAEAGLWMRV
                     QHGHLVICDGYDHNQTPVRADSVWSLLTLDNVNQLDGGWQPVPTDIFRQVTPTERFRG
                     RRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGYSISHCSPQIEEMITQGEF
                     TWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEPQAGPFV
                     SAPADLFDRVQPENRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAW
                     REQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLK
                     EDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTT
                     DRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGEFVCTDYGDRVVFRNHHDR
                     NALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAWHNVTGRTGHEDYRITR
                     PDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG"
     gene            complement(8429..8761)
                     /locus_tag="POB34_000695"
     CDS             complement(8429..8761)
                     /locus_tag="POB34_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HDI5917691.1"
                     /translation="MSDKRQRSGSERRQKTILRAVRFSPDEDETICKNAEDSGLTVSA
                     YIRNAALNKRINSRTDDAFLKELMRLGRMQKHLFVQGKRTGDKEYAEVLVAITELTNT
                     LRKQLMEG"
     gene            8994..9329
                     /locus_tag="POB34_000696"
     CDS             8994..9329
                     /locus_tag="POB34_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HDI5917692.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(9415..10263)
                     /locus_tag="POB34_000697"
     CDS             complement(9415..10263)
                     /locus_tag="POB34_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDI5917693.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRRIDKPEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNACKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPG
                     ALPPRV"
     gene            10612..10809
                     /locus_tag="POB34_000698"
     CDS             10612..10809
                     /locus_tag="POB34_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917694.1"
                     /translation="MQGAPHTARLKKNDGTTMACGGHWHTTPAQVKTEKTARHLQAKK
                     VKFLHFFTLIYRADLAIVMIK"
     gene            10843..11094
                     /locus_tag="POB34_000699"
     CDS             10843..11094
                     /locus_tag="POB34_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI5917695.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
     gene            complement(11125..11322)
                     /locus_tag="POB34_000700"
     CDS             complement(11125..11322)
                     /locus_tag="POB34_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917696.1"
                     /translation="MPGFFVSRSRANSMTIAERLIQKGALEVAREIACRLRDMGWTPE
                     RIQEATGLSGEELKKLFPDEQ"
     gene            complement(11345..12283)
                     /locus_tag="POB34_000701"
     CDS             complement(11345..12283)
                     /locus_tag="POB34_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDI5917697.1"
                     /translation="MSQKKTTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGRMEGRMEGRMEGAL
                     EKALAIACQLQKMGMTPEQIKQATGLSDDELKKITH"
     gene            complement(12348..12614)
                     /locus_tag="POB34_000702"
     CDS             complement(12348..12614)
                     /locus_tag="POB34_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917698.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(12708..13142)
                     /locus_tag="POB34_000703"
     CDS             complement(12708..13142)
                     /locus_tag="POB34_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HDI5917699.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            complement(13871..14371)
                     /locus_tag="POB34_000704"
     CDS             complement(13871..14371)
                     /locus_tag="POB34_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDI5917700.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRLARDEGCEEAYRLWVEDTGETD
                     FDTFRDAWWGEADSEEAFAVEFASDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(14833..15429)
                     /locus_tag="POB34_000705"
     CDS             complement(14833..15429)
                     /locus_tag="POB34_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917701.1"
                     /translation="MMKSDEKYQVPAWMRPLLPLLCNTGGNDPEELLNDTETTASANV
                     VRYVLIVAVRSQVDLLQLLYRKGLLRTEIPGGFSPEEAQALLDNLVRSHISKALSGER
                     MAARDRNADLAWIGQQLVDDAWFVRATLEAHGMSVGNESPPAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSCVVREAQDWLRDRKLVDITDILR"
     gene            complement(15426..16145)
                     /locus_tag="POB34_000706"
     CDS             complement(15426..16145)
                     /locus_tag="POB34_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HDI5917702.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYATAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENVI"
     gene            complement(16142..16576)
                     /gene="psiB"
                     /locus_tag="POB34_000707"
     CDS             complement(16142..16576)
                     /gene="psiB"
                     /locus_tag="POB34_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HDI5917703.1"
                     /translation="MNTELTLNALQSMNAQEYEEIRAAGSDMRRNLTHEVMRKVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPAAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            complement(16631..18589)
                     /locus_tag="POB34_000708"
     CDS             complement(16631..18589)
                     /locus_tag="POB34_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDI5917704.1"
                     /translation="MPVTKCEPETTRKASRKSVKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDATDDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFDSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFLALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGRTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCSPRPQTDATERADNLADAA"
     gene            complement(18648..18881)
                     /locus_tag="POB34_000709"
     CDS             complement(18648..18881)
                     /locus_tag="POB34_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HDI5917705.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDCAGYWMNQYIRDFGILK"
     gene            complement(18939..19466)
                     /locus_tag="POB34_000710"
     CDS             complement(18939..19466)
                     /locus_tag="POB34_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI5917706.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTVQMLGRAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            complement(19850..20164)
                     /locus_tag="POB34_000711"
     CDS             complement(19850..20164)
                     /locus_tag="POB34_000711"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917707.1"
                     /translation="MRGAAPAALPARLSGQKVVYGCMQGTGVIFRDTVTVSGCLLLRP
                     LRLPAAWRHPVVRPDSDRQLLAHPPVSAEPAGAVSGCRYGPMRENPACSDRSGRAAPV
                     AP"
     gene            complement(20235..20426)
                     /locus_tag="POB34_000712"
     CDS             complement(20235..20426)
                     /locus_tag="POB34_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917708.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(20423..20845)
                     /locus_tag="POB34_000713"
     CDS             complement(20423..20845)
                     /locus_tag="POB34_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HDI5917709.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEYGADVSVVHGVVRELMEEKRASRQVTVPAVMLEKVLALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(20892..21194)
                     /locus_tag="POB34_000714"
     CDS             complement(20892..21194)
                     /locus_tag="POB34_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDI5917710.1"
                     /translation="MMPEYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSA
                     DAAGIILTSLVINRQLWLYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(21735..22511)
                     /locus_tag="POB34_000715"
     CDS             complement(21735..22511)
                     /locus_tag="POB34_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917711.1"
                     /translation="MNENTTLNALICRHARNLLLAQGWPEETDVDQRNPNHPGWISIY
                     VLLDAPRLATLLINRHGGALPPLLASAIHKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLAEDEIRAVLDAVRDAVRCVSYQVAEDTRRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSWLAWDV
                     LRIPDEPPRRWFDRGVLREVVREARNEIRSMADALAKIRK"
     gene            complement(22557..22991)
                     /locus_tag="POB34_000716"
     CDS             complement(22557..22991)
                     /locus_tag="POB34_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HDI5917712.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRTESGISSGVAMEIINNVSENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQAVINQARTLLKNNRHEND"
     gene            complement(23005..23226)
                     /locus_tag="POB34_000717"
     CDS             complement(23005..23226)
                     /locus_tag="POB34_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917713.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELEACFKD"
     gene            complement(23227..23910)
                     /locus_tag="POB34_000718"
     CDS             complement(23227..23910)
                     /locus_tag="POB34_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HDI5917714.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMFRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIENF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(23986..24298)
                     /locus_tag="POB34_000719"
                     /pseudo
     CDS             complement(23986..24298)
                     /locus_tag="POB34_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24295..25197)
                     /locus_tag="POB34_000720"
     CDS             complement(24295..25197)
                     /locus_tag="POB34_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HDI5917715.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLL
                     QTTEDVWFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYDLNISSQGDNFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            complement(25878..26270)
                     /locus_tag="POB34_000721"
     CDS             complement(25878..26270)
                     /locus_tag="POB34_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HDI5917716.1"
                     /translation="MYEGEERKKLSLYLHPEDSADCLALAEIETVPRKKRGELYRQAL
                     ITGLIMHQLDERIPAVLTALFTRELNADEVISQIARITGWKPSSGDLKEVLKALGGLQ
                     STVSPEHSQDDGEQARLKAARVKMQNLI"
     gene            complement(26274..27248)
                     /locus_tag="POB34_000722"
     CDS             complement(26274..27248)
                     /locus_tag="POB34_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StbA family protein"
                     /protein_id="HDI5917717.1"
                     /translation="MRIFCDDGSTNVKLAWFEGKTLKSAVSVNSFRHNWKVEGLGSSR
                     TYNYLLDGRKYTYDPVSEDAISTTHIEYQYSDTNVLAVHHALLNSGIEPQEIDLTVTL
                     PISEFYTADCQKNTLNIERKIRNLMREVTLNKGGTFTIKSVEVMPESLPAVFTRLVAD
                     NVGQYEKSLVIDLGGTTLDVGVIVGQFEDVSAVHGNPDIGVSMVTKATLTALKMASSD
                     TSPMIADELIKNRNNLDFVGQVVNEVSKLNLVLDTIDTAINKLGELVVDDLLQYRNVN
                     RVYIVGGGAALIADAVRKAWNHLGEKVVLMDEPQTALVQAIARFKAEE"
     gene            complement(27487..27861)
                     /locus_tag="POB34_000723"
     CDS             complement(27487..27861)
                     /locus_tag="POB34_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917718.1"
                     /translation="MADASKLRTKRTRSGLGVPPGPDEVATSLNAPEIAPAVAATDVQ
                     RQQADSSPVHHEPVVTQTVTEVYTRRDGRSARKTNRTLAFATRVTPAFDQEIRDIAER
                     EGLKLVEVLEKAVEAYKEKQGY"
     gene            complement(27861..28493)
                     /locus_tag="POB34_000724"
     CDS             complement(27861..28493)
                     /locus_tag="POB34_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDI5917719.1"
                     /translation="MAWIVGFISQKGGVGKSTKARALAREASACGIKTKLADLDLEQA
                     TSAEWHRRRLSAGLSPVASVEVFSTAKQAIESAGDFDLLILDGPARASKGTSEIAKVA
                     DLIVQPTGASLDDLVPAIKVFNALVKEGIPRSKLVFALSRVGTEAEELDARAYISEAG
                     YETLSGCLFEKPAYRKAMNSGLAVTETRYKGLNERADELIQALIDKIGEE"
     gene            complement(29077..29862)
                     /locus_tag="POB34_000725"
     CDS             complement(29077..29862)
                     /locus_tag="POB34_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HDI5917720.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLAPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDAWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFAMPEADAVALIKQLERR"
     gene            complement(29864..30277)
                     /locus_tag="POB34_000726"
     CDS             complement(29864..30277)
                     /locus_tag="POB34_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917721.1"
                     /translation="MFFIENEGQAVAGTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGALHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            30846..31106
                     /locus_tag="POB34_000727"
     CDS             30846..31106
                     /locus_tag="POB34_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917722.1"
                     /translation="MDEERVFSLSYEQLTRFTERRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRKLIWPEDDWK"
     gene            31103..31693
                     /locus_tag="POB34_000728"
     CDS             31103..31693
                     /locus_tag="POB34_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917723.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLEVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGIEVRVQVNESVTP"
     gene            complement(31711..32058)
                     /locus_tag="POB34_000729"
     CDS             complement(31711..32058)
                     /locus_tag="POB34_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917724.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLCILWRLGIRLPPLPF
                     MPFWQVTLLMGSLWGISWGCAMWFIYRGPSGMVAGEAIIISITGGFLFGLLTASFHWW
                     RRKVNRLPPWGDV"
     gene            32177..32524
                     /locus_tag="POB34_000730"
     CDS             32177..32524
                     /locus_tag="POB34_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HDI5917725.1"
                     /translation="MKLDISVKYLLKSLIPSLIILTVFYLGWKDSQENARMFYAFIGC
                     IISAITFPFSMRIIQKMVIRFTGKEFWQKDFFTNPVGGSLTAIFELFCFVISVPVVAI
                     YLIFIFCKALSGK"
     gene            complement(32542..34422)
                     /locus_tag="POB34_000731"
     CDS             complement(32542..34422)
                     /locus_tag="POB34_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="HDI5917726.1"
                     /translation="MSDPVRITNPGAESLGYDSDGHEIMAVDIYVNPPRVDVFHGTPP
                     AWSSFGNKTIWGGNEWVDDSPTRSDIEKRDKEITAYKNTLSVQQKENENKRTEAGKRL
                     SAAIAAREKDENTLKTLRAGNADVADITRQEFRLLQAELREYGFRTEIAGYDALRLHT
                     ESRMLFADADSLRISPREARSLIEQAEKRQKDAQNADKKAADMLAEYERRKGILDTRL
                     SELEKNGGAALAVLDAQQARLLGQQTRNDRAISEARNKLSSVTESLKTARNALTRAEQ
                     QLTQQKNTPDGKTIVSPEKFPGRSSTNHSIVVSGDPRFAGTIKITTSAVIDNRANLNY
                     LLTHSGLDYKRNILNDRNPVVTEDVEGDKKIYNAEVAEWDKLRQRLLDARNKITSAES
                     AVNSARNNVSARTNEQKHANDALNALLKEKENIRSQLADINQKIAEEKRKRDEINMIK
                     DAIKLTSDFYRTIYDEFGKQASELAKELASVSQGKQIKSVDDALNAFDKFRNNLNKKY
                     SIQDRMAISKALEAINQVHMAENFKLFSKAFGFTGKVIDRYDVAVELQKAVKTDNWRP
                     FFVKLESLAAGRAASAVTAWTFSVMLGTPVGILGFAIIMAAVSAFVNDKFIEQVNKLI
                     GI"
     gene            complement(34701..36047)
                     /locus_tag="POB34_000732"
     CDS             complement(34701..36047)
                     /locus_tag="POB34_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917727.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKKPATMALHNLA
                     NDILREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSSGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGESSSVRKKRLRLA
                     RLLLSLIDDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRSLSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFEKHMYGVFMHLRY"
     gene            36401..37003
                     /locus_tag="POB34_000733"
     CDS             36401..37003
                     /locus_tag="POB34_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proQ/FINO family protein"
                     /protein_id="HDI5917728.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSLPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRMQAQQENISGQ"
     gene            37059..37553
                     /locus_tag="POB34_000734"
     CDS             37059..37553
                     /locus_tag="POB34_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917729.1"
                     /translation="MCDSEAEKILFILNKHERAYRRLIPVYIFLAIVLWILPILLIVY
                     LYFCIVDNEFLRIGFCIAWTAYYFLSLFKYFVKYFFPLDREDLLKLMELAKDSPDAKQ
                     ELLERLLSGKRMTGRDEMDIKRILKTKEALDNIRNFIDKQQLTNSDTPKKSTVPNEDK
                     KGIR"
     gene            37553..37819
                     /locus_tag="POB34_000735"
     CDS             37553..37819
                     /locus_tag="POB34_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917730.1"
                     /translation="MITGNGINTVTVNGKVKHITELDDITLCLEWTKLREENNRLYEI
                     NNQANRGWRGFILRLIGVNLPDKRTEFTQRLLLTRKISGSVMKK"
     gene            complement(38296..38712)
                     /locus_tag="POB34_000736"
     CDS             complement(38296..38712)
                     /locus_tag="POB34_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004026565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917731.1"
                     /translation="MFRFLRRPLAEVANYSNDRWEAPQRASRLAASVKRYKTSEMLRF
                     IFATIAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVFGEKGRQHRSADSNPEMVEK
                     MAARYRHAAELHWSATLAEIERVKRLYQTKIKKSKK"
     gene            complement(38721..41687)
                     /locus_tag="POB34_000737"
     CDS             complement(38721..41687)
                     /locus_tag="POB34_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HDI5917732.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(41690..42250)
                     /locus_tag="POB34_000738"
     CDS             complement(41690..42250)
                     /locus_tag="POB34_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI5917733.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEPKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(42376..42726)
                     /locus_tag="POB34_000739"
     CDS             complement(42376..42726)
                     /locus_tag="POB34_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HDI5917734.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(42929..43942)
                     /gene="intI1"
                     /locus_tag="POB34_000740"
     CDS             complement(42929..43942)
                     /gene="intI1"
                     /locus_tag="POB34_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HDI5917735.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            44091..44882
                     /gene="aadA1"
                     /locus_tag="POB34_000741"
     CDS             44091..44882
                     /gene="aadA1"
                     /locus_tag="POB34_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HDI5917736.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            45049..45948
                     /gene="aac(3)-VIa"
                     /locus_tag="POB34_000742"
     CDS             45049..45948
                     /gene="aac(3)-VIa"
                     /locus_tag="POB34_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(3)-VIa"
                     /protein_id="HDI5917737.1"
                     /translation="MTDPRKNGDLHEPATAPATPWSKSELVRQLRDLGVRSGDMVMPH
                     VSLRAVGPLADGPQTLVDALIEAVGPTGNILAFVSWRDSPYEQTLGHDAPPAAIAQSW
                     PAFDPDHAPTYPGFGAINEFIRTYPGCRRSAHPDASMAAIGPDAAWLVAPHEMGAAYG
                     PRSPIARFLAHAGKILSIGAGPDAVTALHYAEAVARIEGKRRVTYSMPLLREGKRVWV
                     TTSDWDSNGILDEYAAPDGPDAVERIARDYLARTRVAQGPVGGAQSRLIDAADIVSFG
                     IEWLEARHAAPAAAALKPKQRRD"
     gene            46155..46475
                     /gene="groES"
                     /locus_tag="POB34_000743"
     CDS             46155..46475
                     /gene="groES"
                     /locus_tag="POB34_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDI5917738.1"
                     /translation="MGSPTRKICAAINIKPLYDRVVIKRTEEEKVSAGGIVIPDSATE
                     KPIKGQVVAVGEGKVFDNGNVRAPKVKVGDQVLFGKYAGTEVKLDGTEYLVVKEDDIF
                     AVLG"
     gene            46531..48168
                     /gene="groL"
                     /locus_tag="POB34_000744"
     CDS             46531..48168
                     /gene="groL"
                     /locus_tag="POB34_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411226.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDI5917739.1"
                     /translation="MAAKDIRFGEDARARMVRGVNVLANAVKATLGPKGRNVVLEKSF
                     GAPTITKDGVSVAKEIELADKFENMGAQMVKEVASKTSDNAGDGTTTATVLAQALIRE
                     GMKAVAAGMNPMDLKRGIDKAVTSAVEELKKISKPCSTSKEIAQVGSISANSDTDIGE
                     LIAKAMDKVGKEGVITVEEGSGLENELDVVEGMQFDRGYLSPYFINNPQSMQAELEDP
                     FILLHDKKISNVRDLLPILEGVAKAGKPLLIVAEDVEGEALATLVVNTIRGIVKVCAV
                     KAPGFGDRRKAMLEDMAILTGGTVISEEVGLSLEKATINDLGRAKKVQVSKENTTIID
                     GAGDTADIEARIKQIKAQIEETTSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGIVPGGGVALIRAKAAIAELKGANEDQNHGIAIALRAMEAP
                     LREIVTNAGDEPSVVLNRVAEGTGAFGYNAANGEFGDMIEFGILDPTKVTRSALQNAA
                     SIAGLMITTEAMVAEAPKKEEPAAPGGGMGGMGGMDF"
     gene            48357..49886
                     /locus_tag="POB34_000745"
     CDS             48357..49886
                     /locus_tag="POB34_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HDI5917740.1"
                     /translation="MPRLTAPRRQAEVCAPLPAAHAAARYARHLPERTLLYALVQAHY
                     PDFIARLEAEDRPLPEYVREEFETYLRCGVLEHGFLRVVCEQCRAERLVAFSCKKRGF
                     CPSCGARRMAESARHLVEEVFGPRPVRQWVLSFPYPLRFLFASKPEAIGPVLGIVHRV
                     IAGWLADQAGVDHASAQCGAVTLIQRFGSALNLNVHFHMLWLDGVYEDTTERPQRKPR
                     LHRTRAPTSAQLTELAGTIAHRVCRHLSRRGWLEGEDESVFLSDSAGSDDGMDGLRMS
                     SMTYRIATGRDAGRKVVTLQTLPGDAGSLEGDAGKVGGFSLHAGVAAEAHESHKLEKL
                     CRYITRPAISEQRLSISPQGRVRYQLKTPWRNGTTHVEWDAVDFIAKLAALVPPPRAH
                     LTRFHGVFAPNANLRAQLTPSGRGRRPAGDAVPVDASAHDEPRSPEQKRRAMSWAQRL
                     KRVFSIDVTTCAHCGGAVRIVASIEDPKAIRAILAHFEKHGALEQAHYRPAARAPPPA
                     A"
     gene            50154..51389
                     /locus_tag="POB34_000746"
     CDS             50154..51389
                     /locus_tag="POB34_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEc58 family transposase"
                     /protein_id="HDI5917741.1"
                     /translation="MPRKKKTPVVTPAIDLPKEFLEKLIPGPMDAAGVEAVFQQLKKA
                     VIERALGAELGLHLADAEGGSGNHRNGRSGKTVLTDEGPLRIDVPRDRTGTFEPQLIP
                     KHERRFAGFDDRIVSMYARGMTVREIQGHLAEMYSVEVSPEFISKVTDEVMAEVTAWQ
                     ARPLEAMYPVVFFDALRVKIREDGVVRNKAVYLALGVLADGTRDILGLWIENTEGAKF
                     WMKVFNDLKTRGVNDILIGVADGLKGFPEALEAVFPATTLQTCIVHLIRNSLDYAGWK
                     DRKALAAAIKPIYTAPTAEAAEAELDAFEAGDWGERFPTVVAAWRRAWTRVVPFFAFP
                     PDVRRVIYTTNAIESVHARLRKIIKTRGHFPSDEAATKLIWLALRNITAEWSRASQHW
                     KAAMNQFAIIYEDRFTLAR"
     gene            51607..51954
                     /locus_tag="POB34_000747"
     CDS             51607..51954
                     /locus_tag="POB34_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HDI5917742.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            51948..52787
                     /gene="sul1"
                     /locus_tag="POB34_000748"
     CDS             51948..52787
                     /gene="sul1"
                     /locus_tag="POB34_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HDI5917743.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            52915..53415
                     /locus_tag="POB34_000749"
     CDS             52915..53415
                     /locus_tag="POB34_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5917744.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAEDPEFWPDALKGEAAYLHKLAVRRTHA
                     GRGVSSALIEACRHAARTQGCAKLRLDCHPNLRGLYERLGFTHVDTFNPGWDPTFIAE
                     RLELEI"
     gene            complement(53591..54373)
                     /gene="istB"
                     /locus_tag="POB34_000750"
     CDS             complement(53591..54373)
                     /gene="istB"
                     /locus_tag="POB34_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS1326 family helper ATPase
                     IstB"
                     /protein_id="HDI5917745.1"
                     /translation="MQHEGHVRILKSLKLFGMAHAIEELGNQNSPAFNQALPMLDSLI
                     KAEVAEREVRSVNYQLRVAKFPVYRDLVGFDFSQSLVNEATVKQLHRCDFMEQAQNVV
                     LIGGPGTGKTHLATAIGTQAVMHLNRRVRFFSTVDLVNALEQEKSSGRQGQIANRLLY
                     ADLVILDELGYLPFSQTGGALLFHLLSKLYEKTSVILTTNLSFSEWSRVFGDEKMTTA
                     LLDRLTHHCHILETGNESYRFKHSSTQNKQEEKQTRKLKIET"
     gene            complement(54363..55886)
                     /gene="istA"
                     /locus_tag="POB34_000751"
     CDS             complement(54363..55886)
                     /gene="istA"
                     /locus_tag="POB34_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS1326 family transposase"
                     /protein_id="HDI5917746.1"
                     /translation="MINVAILSAIRRWHFRDGASIREIARRSGLSRNTVRKYLQSKVV
                     EPQYPARDSVGKLSPFEPKLRQWLSTEHKKTKKLRRNLRSMYRDLVALGFTGSYDRVC
                     AFARQWKDSEQFKAQTSGKGCFIPLRFACGEAFQFDWSEDFARIAGKQVKLQIAQFKL
                     AHSRAFVLRAYYQQKHEMLFDAHWHAFQIFGGIPKRGIYDNMKTAVDSVGRGKERRVN
                     QRFTAMVSHYLFDAQFCNPASGWEKGQIEKNVQDSRQRLWQGAPDFQSLADLNVWLEH
                     RCKALWSELRHPELDQTVQEAFADEQGELMALPNAFDAFVEQTKRVTSTCLVHHEGNR
                     YSVPASYANRAISLRIYADKLVMAAEGQHIAEHPRLFGSGHARRGHTQYDWHHYLSVL
                     QKKPGALRNGAPFAELPPAFKKLQSILLQRPGGDRDMVEILALVLHHDEGAVLSAVEL
                     ALECGKPSKEHVLNLLGRLTEEPPPKPIPIPKGLRLTLEPQANVNRYDSLRRAHDAA"
     gene            complement(55988..56848)
                     /locus_tag="POB34_000752"
     CDS             complement(55988..56848)
                     /locus_tag="POB34_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniB family NTP-binding protein"
                     /protein_id="HDI5917747.1"
                     /translation="MDEYPIIDLSHLLPAAQGLARLPADERIQRLRADRWIGYPRAVE
                     ALNRLEALYAWPNKQRMPNLLLVGPTNNGKSMIVEKFRRTHPASSDADQEHIPVLVVQ
                     MPSEPSVIRFYVALLAAMGAPLRPRPRLPEMEQLALALLRKVGVRMLVIDELHNVLAG
                     NSVNRREFLNLLRFLGNELRIPLVGVGTRDAYLAIRSDDQLENRFEPMMLPVWEANDD
                     CCSLLASFAASLPLRRPSPIATLDMARYLLTRSEGTIGELAHLLMAAAIVAVESGEEA
                     INHRTLSMAC"
     gene            complement(56851..>58441)
                     /locus_tag="POB34_000753"
     CDS             complement(56851..>58441)
                     /locus_tag="POB34_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI5917748.1"
                     /translation="ISPLAQSETVGHEAADMAAQALGLSRRQVYVLIRRARQGSGLVT
                     DLVPGQSGGGKGKGRLPEPVERVIHELLQKRFLTKQKRSLAAFHREVTQVCKAQKLRV
                     PARNTVALRIASLDPRKVIRRREGQDAARDLQGVGGEPPAVTAPLEQVQIDHTVIDLI
                     VVDDRDRQPIGRPYLTLAIDVFTRCVLGMVVTLEAPSAVSVGLCLVHVACDKRPWLEG
                     LNVEMDWQMSGKPLLLYLDNAAEFKSEALRRGCEQHGIRLDYRPLGQPHYGGIVERII
                     GTAMQMIHDELPGTTFSNPDQRGDYDSENKAALTLRELERWLTLAVGTYHGSVHNGLL
                     QPPAARWAEAVARVGVPAVVTRATSFLVDFLPILRRTLTRTGFVIDHIHYYADALKPW
                     IARRERWPSFLIRRDPRDISRIWVLEPEGQHYLEIPYRTLSHPAVTLWEQRQALAKLR
                     QQGREQVDESALFRMIGQMREIVTSAQKATRKARRDADRRQHLKTSARPDKPVPPDTD
                     IADPQADNLPPAKPFDQIEEW"
BASE COUNT        13150 a        16606 c        15781 g        12904 t
ORIGIN      
        1 atatgccgga cagaacatgc ctcagagctg acatgccgag tatacactta tccagtactt
       61 gcagaaatat aattccagac gtactatcta caggtcagtt atagacagtt cttttccgac
      121 cagagccacc cactctgtaa cggaacagta aaaaaaccga cctcaccagt cggttttttt
      181 acatctgttg tttctcaaaa tccgcccgac atgcccgtca caccggggaa actcatgctt
      241 tgaatcagaa ccaaccatct caactttgcc gtctcctgaa aaaactggca cattcagttc
      301 gataactacc cataaataca gagagaaaat ccgatgtcag aaaagtggag taaagaattc
      361 gactcttgct ttcaggtggg gagcatagaa taaattgccg atgctctcga gaaacgcagc
      421 ggaagcgagg aaaatgcgtt agccatgctg gcaatgacag ccttcacact catgacccga
      481 cgtggtatct gcgaaatgca gaacgtggcc ccggatggca aaggcattcg gctggagcta
      541 atcggcttca ggggaaatga aacaataccg gatacgcttc attgaagaag aaagggcgca
      601 atgaggtcag ccttcgcaac aaagcggcgg cagaaaacct gccgccgtgt tgcccgttaa
      661 cgtttaactt cgtaggctaa cgttgccaca agcactgtgt ttccctgctg gagccggagt
      721 ccgcaaagcg aatccctcac catccagaca aaacacagaa tcgtcacaca gatgacgatt
      781 aacatcatta aaaacgttcg ctgtggcatc atagcccctc cattgcctta cggcggttat
      841 cgggctacta taatgttgtc taggcattac agtagagccc ttggttgatt gataaaaaaa
      901 tctccagggg ctttctgctg tataccgtcc acaaaacgct ttcggcatac agcctcaagc
      961 gcccgcagct attctaacga ctttcgtatc tgcaacaatc cttttatcac acggctaatt
     1021 cctctggtta gctcttatcg cctgcaaata aatttttaac ctttccgttt gatttaaatt
     1081 ccagaaatgg agactttgtc atgtcttaca atcgacagcc tgtcgcagaa gatcctatgc
     1141 agatatgggg cgctgttggt gtccttctta tcctgctgct attcgttata tggttattct
     1201 tgccggaagt ggtttacgcc agctgcctga ttcttcatac cttatgggga ctggttgact
     1261 ggggaccatt tcataactat gcagcaccac gatacaacct gcttgccatg accggtaaca
     1321 acgccgccaa cataagctat agccagtggg ttaacgtcat ggagcagacc ataggtattc
     1381 tgtggatgta cctcttacct gtgacactct ggtgtttgtg ggagtggtat caacatccgg
     1441 gacaaagtcg tttcacccgc agaccagtag atattacccg cctgccatac atctttgctt
     1501 cgttgtcccc tgctatcgcc cctgtactgg cagatggtga tccggaaaaa ctctttcatg
     1561 gtggcaaacg acctgaacgg cgggttgctc tcaccccgga agcatttgtc gagcagcata
     1621 ctctcatcac caatatgcag ctcgatgttg cagctgcaag acgatgtttc atggcccagt
     1681 tgggtaaacc tttaacgtcc tggaaagaca tggcaccaca tgaaaaagcg ctctttgcca
     1741 tatttggtct tcagtatttc ctggatgatc gtaaggcagc attaaagctc atggatacac
     1801 tgaacctctc atgcagaatt aaaagcaaac gggacagtgg gaaattctgt acccctgttt
     1861 attcactggc caaatcagct ttccagagag tgattaaaag caacggagca cagcaatggc
     1921 tgaaacaaca tcgctacgtc cgcagtgggc tggtctggct gtatgcacat gacctgcggc
     1981 ttacgccacc caactggatt tggctgaaag gcgtggacag gacgctgttt tatgcattac
     2041 accgcgccaa taccacgaaa ggatttattg aaggcgctgg cgtggtggcc gttgcacgtg
     2101 cagaggccga agccatgcgt tttggccttc cctgccctga accctgtgtt gatgaagcgg
     2161 tggaaggctt acggcgggat atgctcagtc tcggtcttat ctgggatgaa ccacaaccag
     2221 atcgcgaccg gaaacgccgc atcctgacca actggtccct caccgacgac atcctgcccc
     2281 gcacacctgc tacagacaac gaattctgat tatccggaga actgaaaatg acaatcgagt
     2341 actttgcaac acgtattgca aaacatattt ccgtgcaggc aatctggccg gatggcagga
     2401 cagaaattat tgccgtcctt ccacgggatg ccctgtcagg gctggtaaca cgaaataatc
     2461 aactgattca tatagccttt gacgccatgg gcacccgtaa cagagaaacc gtcctgattg
     2521 ataaacagga gcataacgct gaccagatac ttacagctat tgattcctgc ctgcgccttg
     2581 aatatctgat ggaacgccgc ttcagcagaa cacctttgtt ccgtggcata atcgccagcg
     2641 ttgtgctgtt cgtaatggca acgattggct tctctctgtt ccgctatgtt gatcgtgtat
     2701 tctgggatga tacaacgcct gaagcagtac agacagcagg tgagccacga ctgcttcccc
     2761 ctcacctgaa tcacactgtg ccattaaatg aaggcattca actcccggtt cccccgaaaa
     2821 aagacgtcca ggtaccggag aaaacaatca caagcaaaaa tccggaagcc gcagctgcgc
     2881 gacataatct ggctactgtt ctgaagcgga acgccgatcg gggcatgttt accatcaacc
     2941 tgtcatccgg tcatgaacga accctgttcg catttctcga tcctgcctgt cctaactgca
     3001 gacttctgga gccggcctta aaacgcctgg ccagcgactt caatgtcgtt atctacccgg
     3061 tttcagttat tggtggagag gaatccaccg accgtgtggc accactgctg tgtgaaaaag
     3121 atgctcagaa acgtgctgcc ggctggcacc gactgtattc cgcagataac ggaatgatga
     3181 cgccatcaga agaaacaacg cctgcagatg aaacatgcct gaaagcagcc agagctgcga
     3241 ttgatgttaa caatgtcgcc ttccgtaagt tcggatttgc cggtacccca tgggtgctgt
     3301 ccgacaccgg ctggcatctg cctacaggaa ttctgcagga aactggcacg cttaatttat
     3361 tcctgaaaac caccgacagc gagtctggcc atgagtgaac atcgcgttaa ccccgagctt
     3421 ttacatcgta cagcctgggg aaatcctgtc tggaatgcac tccagagtct caatatctat
     3481 ggcttttgcc tggtggcaag tcttgtggcc agttttatct ggccacttgc attaccggca
     3541 tgtctgttgt tcacactgat aacaatgctg gtatttagtc tgcagcgatg gcgctgccct
     3601 cttcgtatgc ccatgacgct ggaatgtgct gacccgtcac aggacagaat gattaaaaga
     3661 agtcttttca gcttctggcc aaccctgttc cagtatgagg tcattctgga atcaccggcc
     3721 agcggcatat tctatgttgg ttatcaacgc gtcagggata ttggcaggga gttatggctg
     3781 agtatggacg atctgacccg ccatataatg tttttcgcca ccaccggtgg cggtaaaacg
     3841 gaaaccatct ttgcctgggc gattaaccca ctttgctggg caagaggatt cacccttgtc
     3901 gatggcaagg cacagaatga tacggcaaga acgatctggt atctggccag acgctttgga
     3961 cgtgaagacg atgtggaagt cattaacttc atgaacggag gaaaatcccg cagtgaaatc
     4021 attctgagtg gtgaaaaaac gcgtcctcag tcaaatacct ggaatccctt ctgttacagc
     4081 accgaggcat tcacagcgga gacaatgcag tcaatgctgc cccagaacgt ccaggggggc
     4141 gagtggcagt cccgggccat tgccatgaat aaggcactgg tatttggtac taaattctgg
     4201 tgtgtgcgcg aaggcaaaac aatgtcgctg cagatgctcc gtgaacatat gactctcgaa
     4261 gggatggcca aactttactg tcgtggcctc gatgatcagt ggccggaaga agctatcgca
     4321 ccgttacgta actaccttca ggacgttccc ggtttcgacc tgtcgctggt caggacacca
     4381 tcagcctgga cggaagaacc cagaaaacaa catgcttatc tctccgggca gttttctgaa
     4441 actttcagca cattcacgga agcattcggg gatatttttg ctgaagattc cggtgatatc
     4501 gatatccgtg acagcattca cagcgacagg attctgatgg taatgatccc cgctctggat
     4561 acatcagcgc atacaacgtc tgctcttgga cgaatgttta tcacccagaa aagtatgatt
     4621 ctcgcccgtg atttgggcta ccgcctcgaa ggaactgatt ccgatgcact ggaagtcaaa
     4681 aaatataagg ggcgtttccc ttatctgtgt ttccttgatg aagtaggggc atactatacg
     4741 gaccgtattg ctgtggaagc cactcaggtg cgttcactgg attttgctct gattttgatg
     4801 gcacaggacc aggaacgtat agaaggacag acaacagcca cgaatactgc aacactgatg
     4861 cagaacacgg ggacaaaatt tgccggcagg attgtcagcg agggtagtac ggccagaacg
     4921 ctgaaaagcg ccgccggtga agaagccagg gcgagaatga acaacctgca gcgtcaggac
     4981 ggcatatttg gtgaatcatg gattgacagc ccacaaatca gcatcctgat ggaaagcaag
     5041 ataaatgttc aggagttgat tgaacttcat cctggcgagt tcttttctat tttccgggga
     5101 gaaaccgtac catcagcatc attcttcatc cctgacgatg agaaaagctg cagcagcgat
     5161 cccgttgtta taaacaggta tatcagcgtg gatgcgccac gcctggaccg tctccgccgt
     5221 cttgttcccc gtactactca acgtcgaata ccgtcaccgg aaaatgtcag tgccatcatt
     5281 ggcgtcctga ctgcaaaacc ctccagaaaa agacgaaaaa ttcgcacaga gccgcacacg
     5341 atcgtggata catttcagca acgcattgca ggacgtcagg cggctatggc aatgctggaa
     5401 gagtacgaca cagatatcaa cgcacgtgaa agtgccttgt gggaaacagc ggtgaatacg
     5461 cttaaaacga ccacacggga agagcgacga attcgctata tcacgctgaa cagaccagaa
     5521 ctacctgaaa caaaagagga aaatcagata tcagtgagag cagaaagagc aggtataaac
     5581 ctgttaacgc ttccacagga caataaccac ccgacagggc gtcctgtaaa cgggtttcat
     5641 cataaaaaaa ccaaccgtcc ggactgggac ggaatgtact gaacagtggc cgcgccggat
     5701 tctggcgcgg cctttttttt aacctggaga aggcggttcc cagcgtttat cctgctccgg
     5761 cggaggcagt gatgcgtcat cgttgctgtt cgccgcgata taatcgcggt agtagcgttc
     5821 acttccttca cggtgatgat ccacgtccgg tcgcgtaatc ctgtagtctt cgtgaccggt
     5881 acggcctgtc acattatgcc aggcaaccat ttcagcaaag acctggttaa attgtttgtc
     5941 gtttccttct ggttcaaacc ccatgcgtgg atcacgcccg aataataccg gtgcaatcaa
     6001 atccagttta tctgccagcg cattgcggtc atggtgatta cggaaaacaa ctctgtcacc
     6061 ataatccgta cagacaaact ctcccgtccg acggtcacgg aaacgaacac tcccgttctt
     6121 ctgcagacgg gcctcgagat caccggtatt accgtatacc ggcgttccgc ccggttcaca
     6181 aagcacgaca cagcggtctg tcgtcgttgt gcgtgacttg tctttacggc gatcgcgata
     6241 atcccagcct ctcaactgcg atacagcagc cctgtctccc tgagcagcct gaatttcgac
     6301 ccactgacgg taagagggtg gataccattt cccgtcagca ataagtttct gtcgctcatc
     6361 cctgatgtct tctttcagcc tgatcaatgc ctgcattcgc tgaacttctg caatgtgata
     6421 atgcagctta cgtaatgccg gatcatcgta ctgcgcacga atgtgtgact tccgcagacg
     6481 gcatgcctga tgaatttccc gacaacgttc cccataacgc agatccggtt tacgccattg
     6541 ttcacgccat gccagatagc gggcacgcag gtcagcacgg gcctcagccc tggcttcacg
     6601 tcgctgccgg cgcagcattg ggtcacgttt actgctgacc ccgtacctgt cactgacagc
     6661 cagctccggg ttatagcgat tttcaggttg caccctgtca aacaaatctg ctggagcact
     6721 cacaaaaggt ccggcctgtg gctccgcacg ccccagtgtc agatcaggat gaatgctgct
     6781 ggctttcacc ggcgtttgct catgattaaa agcatcaacg acaacaagcc cgtgatgttg
     6841 tttctgtagc atcagtccct gttgtgcaaa caactcatga caacgctgcc aggtaaactc
     6901 accctgtgtg atcatttctt caatctgagg gctacaatgg ctgatgctgt atccccacag
     6961 actttctttg tcagaaaaca gttcgcgttt gatagccccc tgcggccccg tacctgtacg
     7021 catacggtgc cattctttat cggtcgcagg acagctctcc atacgacgac cacggaagcg
     7081 ttctgttggc gtaacctggc ggaaaatatc tgttggtaca ggctgccagc caccatcgag
     7141 ttgattcaca ttgtcgagcg tcagcagtga ccatacgctg tcagcacgaa cgggagtctg
     7201 gttatgatca taaccgtcac agataaccag gtgaccatgc tgaacacgca tccataatcc
     7261 cgcttcagcc agtgtgcggt gaatagccag gcagttttcc agccgtcctt cccgtgccat
     7321 ttccggcaga cgttctccaa gatgacgaca ggcatactgc tgcagacttt ctgtttcagc
     7381 gattttttcc gggcgaacat cagctgcaat gaagtccgga ttatagcgtc ctggtgcttc
     7441 cacctggctg aaaatgtctt ttggcactgg cgtgtagtca cccatttttt tcatgagttt
     7501 ttctgcacac caggagggac caaacgagtc aagctgtaca ccttccctgt tgcgatccca
     7561 gccatccatc accagaaatt ttccgtccat ctgagacagg tacagaccat cttccgcaag
     7621 acgacgatgc agggataacc agtcatgtac aggttcactg cgtaaaccag cgaccgctgt
     7681 ctgcgcaaca tactcacgaa aagtttgttt ttttccgcgt gtccaggcgt tctggcgatc
     7741 acgttcaacg gcagttttgc gaacgatacg atttcccggc gcatggaccc agcaaccgtt
     7801 atccggagca aaaccatgct tcagttcaag ctcacggcag gcacgactga gtttttcctg
     7861 actccatgat aaccggttca gataacccgt ttcagggtgt acccggttaa cagccacatg
     7921 gacgtgcaga ttatctgtat cagtatgcac ggcagagaca tactgatggt cggcaaggcc
     7981 gagtgatttc agcgtatgac gtacactgtc ataaatctgt tccggacgcg ggctttcatg
     8041 tgactgccag gaaaggatgt agtgaaaaac agggtcagtg tcatcttttg catagtgtgc
     8101 ctgccgggca atgtactcca tatctgcagc ggcagtttca agagaagtac agttatgaaa
     8161 gcaggtgaca ccataaaagt tgacccattc gcagccgtcc ttcatgacat caaccagcgc
     8221 cacgaatgac tcatttcgaa gacgtgtcgc ataatcaaca aggcgactga aacggcttcg
     8281 gtgcggttgt tcagcctgcg aagaagaaga taaatccagt tcctcatcgg tcatgtcatc
     8341 ccggacagag acgtaggata ccaggtcttc gaacgaagac ttgccgtccc ttctcttttt
     8401 cggaatgact gcattcaccg gagaatcctc aaccttccat taactgttta cgcaatgtat
     8461 ttgtaagttc ggttatggca acaagcactt ctgcgtattc cttgtcaccg gttctttttc
     8521 cctgaacaaa cagatgcttc tgcatccttc ccagtctcat taactccttc agaaaagcat
     8581 catctgtacg ggagttaatt cgcttattca gtgctgcatt tcggatgtaa gcagatacag
     8641 taagtccgga atcttcagca ttcttacaga tagtttcatc ctcatccggt gagaaccgga
     8701 ccgctctcag gatcgtcttt tgccgtcttt cactaccact tctttgacgt ttatcactca
     8761 cggtcagctc cttactgggg tgtcggggcg aagccctgac caggaggcaa ttgtataatc
     8821 gcgcgtgcgc ggttatacaa ttgcacatcc tgtcccgttt ttcaggccat tatagcctgt
     8881 tatttttgtg catcatagtc ttatgcagac ggcagaggca gggcgcagcc ctgccaggag
     8941 tcagtttttg atgataaaat cagacttcaa catgaaacgg agagaaagtg ccaatggctt
     9001 ctaaaaaatt ttactcagat gatgacatcc agctggcaaa agctgcttta tcggaattgc
     9061 cagatctcac cgctcaacgg aaaacattgc gtgattttct tgatgccatt cgtgatgaca
     9121 ttattgtcct ggtaaggaca aagggttata cactggcaga tgttcgtgac acgctcaaaa
     9181 atgcagggta tgaagtagga gagaaggcac ttcgtgacat tattcgggaa gcagaatcga
     9241 aaaaaccatc ccgccgttct tcctctaaaa cagcacctaa aaagacagat tcaggcagga
     9301 aagatggcat cgatatgaac aacaattgat gtgacaatgt cacatcgtgt tctgagatta
     9361 tgatgattac agaaaaaaaa gccccggcat aaataccagg gcttttctgt ttaatcagac
     9421 tcgtggcggc aatgcacctg gataatgccg tgcaataata tcattcagtt tttcaacaag
     9481 gtcagaacgc ttaaagacaa tgtgtacggt ccctttctgg tagccacgca ccgtaaaata
     9541 ttccccttct aacagcttac cgacattgtc gccgttcccg agaaattcac catactgcgc
     9601 cccgatactg ttcctgaagt caggaacgtt gcgcccctcc agcagataaa acggacgtac
     9661 caggtcatcc agtgcctccc gtccgctgca gtccagcgtc acacgcccgt tactccagcg
     9721 gtacagcagg ttttcaataa tgattttctt acccagacgg cagggattat tggttctgta
     9781 atcccatgac aatttccggt agacatcaat cagtccctgt tcaaatgtct gcatcttgca
     9841 ggcattcaga tggcggaatg ttgccagtac attgtccagg gtgatttcag gacaggtgtc
     9901 gctcatcagc tggctgttcc actcatcacg ctggcatgaa ctcatgaagg tgtacatgcc
     9961 cgtatctgtc atcaggcggt cccagatttt ccggtcggca agacgggtga tattcctttt
    10021 cgcttcttcc ggtttatcta tgcgacgaac ggaatcagtc acgcagtctt caaacccgta
    10081 aagaaaaata tcacctgacg catcaagcag aatcctgcgc gctgtcgcca ggcattccat
    10141 cgcctgcatg aacagtgcaa taccttcgtt acgctgcgcg atgatgcggt cgatggccac
    10201 agacggaata acatctgtcg tgccggcagt attcaggtca gcagtgggta aagtctgatt
    10261 catcacattg tctcctcatc gtcacgactg ccgggacagc caggtcattt cccggaggag
    10321 cgaagctcct tctgccgtcc gggtcttcgg ggatttcccc gcagtggacc tttccgacgg
    10381 gggacgctca ccacgtcacg gggcagttgt ccgggggcag acgcaaaagt ttttgacgcc
    10441 tgcggcaaaa agttttgtgg ctggtacatc tcccccctgg acgactgaaa cgggatgtgg
    10501 tcatgtatgt acccccggaa agtccgtctg cggagaacag gccgcagtgc ggcggcagac
    10561 aggcagagac agctgcgcca tcaggcgcag tggccttaca caccgtaagg tgtgcaggga
    10621 gcgccgcata cggcgcgact gaagaaaaac gacggcacaa caatggcatg tggtggtcac
    10681 tggcacacca ctcccgcaca ggtgaaaaca gagaaaaccg cacgacattt acaagcaaaa
    10741 aaagtgaaat ttcttcactt ttttacactt atttatcgcg ctgatttggc aattgtgatg
    10801 ataaaataaa gtgaagtttt ttcacttttt caaaggtgtt ttatgaccgg ctgggaactg
    10861 cgaatctggc gaaaaagcat gctctggtca cgcgaaaaag cggccagaga atttggcgtg
    10921 acgcagagaa catggcacgc ctgggaaaat gcggaacagg tggacgtcac cgtctggagg
    10981 accacccagg cactttcagt ccgtgacctg ctgcctcaca tgcaggggat gcgtaaggca
    11041 gatatcatca ggcgactgga aaatgaactg ggggaaacgg cagaggatgt gtgagcgtga
    11101 ttcacagcct ggctgaatgc caggctactg ctcatcagga aacagctttt tcagttcttc
    11161 accggaaagt ccggttgcct cctgaatccg ttccggcgtc cagcccatat cccgcagccg
    11221 gcaggctatt tcccgcgcca cttcaagtgc gcctttttga ataagacgtt ctgcaatcgt
    11281 catgctattt gctctgctcc tgctgacaaa aaagcccggc attttgccgg gctgtgatgt
    11341 ttactcagtg agtgattttc ttcagttcgt catcggaaag tccggtagcc tgcttaatct
    11401 gctccggcgt catccccatt ttctgaagct ggcacgcaat agccagggct ttttcaagcg
    11461 ctccttccat gcgtccttcc atgcgtcctt ccatgcgtcc ttccatcctg ccctcctggc
    11521 gacctttttg ctcaagacgt tctgcgatag tcatcagttt atcctcatgc tgcggcagac
    11581 gctcagccag cgcccgcata aattccggtg atacagagtc ccctgcgtta acgatgtaat
    11641 gaatcagaac acgcacctgc tcgtctgaaa tttccaccat gacctgcggg agtttgtcca
    11701 gcagctccat catgtcacga tggcggatat gcttcatcag cagcgtcagt gcggccatcc
    11761 ggcgatggtt catgatttca ttgtcatcaa cgacagtgat atcaaccagc ggaaacggct
    11821 ttgtgtatat tttagccgca agtgccggat tctcaaaaca gtccagccag ttcatgctgt
    11881 acgggtacgg gctgcgctca ccatgataga acagtacagg aatcaccagt ggcaacgttt
    11941 tgtgctgctc cagatgtctc tgcatcgccg ccacactgta acgtaacatg cggaaaggca
    12001 tgtacagatt ttcggtgctc tggtgctcga tgagcgtata aacatagcca tcttcacccc
    12061 cggtggtttt cacactccag aggatatcgc tggcgtactg atgcaggtcc ggctcaacaa
    12121 aggtggcggg ttccagcttc agcgtggaca ggtcgcacaa ctgccggtac tccgccggaa
    12181 gatgcagttc cagaaaatct ctggcgacgt cgggattcgc caggaacgac cggaacgcgg
    12241 catcatgcgg cgtgggcgtc gtggtggttt tcttctgact cacggttcct cctggtgaga
    12301 tgtctgaacg tatcctgccg cctgcacagg cagacggcaa gggttaatca gtggcagacc
    12361 cgctcaattt caagcgcgcg gagcaggtgg ccccattcgc tgactttcat agcggggata
    12421 cgcacccgct tctgctcact gcagcgtctg gcgatatggc cgctcatcgc caggacgtcc
    12481 gggttaatct catcctgtgc cagctcctga cgtctgaagc gggtatggcg gtgcaggggt
    12541 ttattctccc gcaggcactc ttcacgcaga cgctttacat actcggcggt tgcgaagata
    12601 attgatggtc tcatggcata atctccatag atagtgattg agatgccgga cttcctggcg
    12661 gggaagcgcc ggcggagaga ggcagggttt atcgccctgc ctttttttca tttcggacag
    12721 cgacgcagtg ccagcgcgag cgctttcatc ttccagcctt ccggcgtgcg gtacatcacc
    12781 ttccagtagc cgtcgatgaa cacataatgg aagcgcacct cttcagtgct ttcagtggca
    12841 atagcctccc tgaaagaacg gtactcacgc gcctcactgt ccatccggaa accactgtca
    12901 cgtccgtaat aagccgtcac accgtccacc gggttctgaa gcgtatgccc ggcgggtttg
    12961 tcgcaacggg gaccgaggca acggatatca ccgtgttcaa ccagggcttc ggcctgctga
    13021 ccggtggcat agtgggtatg caggatgcga ccgttccagg tcggctcccc gtgcaggtgg
    13081 cagtaaacgg tacggaacac cccgtcgcgg caaaaaacgg aaatcgtgct gctgactgtc
    13141 atgaaaacgt ctccttctgc tgaatcaggt cgccgggccg accggttgct ccccggaggg
    13201 gcgaaagccc cttctgccgt ccacttcttc ggggtttctc cccgcagtgg acctttccga
    13261 tggggcgtcc gtcaccacgt cacggggcag ctgtccgggg gcggacgcaa aattttttgc
    13321 cgggtttatg gctaaaaatt ttgtggacgg tatatcttcc cctggactgc tgaaacggga
    13381 tgtggttact ccagcccccg gaaagtccac agcgggaaac aggccgcagg ggtggcggcg
    13441 gaagggttga ctgcgccatc aggcgcagtg gccttacaca ccgtcaggtg tgcagggagc
    13501 gccgtttccg gcgcgacagg ggcggcgcga ccggaacggt cgctgcaagc ctggtttttc
    13561 agacatcgga ccgtagcggc agcccgaaac cgcgccagcg ggttcggcgg agactggcgg
    13621 gtgcgccagc agctgcctgt cggagtcggg cctgacgacc ggatgccgcc aggcagccgg
    13681 gagccgaagg tgtccccgcc tccggaaatc agaacaacag aaacgacata acagaaaaat
    13741 gcctcacacc atgacgggca ggaagggcag cgccgcaggc gcggccctcc tgcatttcag
    13801 gcaggcgtaa gcctgccagg gttttccggc ggcctggcag gccatacccc cgccggagcg
    13861 ggggagtaaa tcaccgacgg aacacatgac cgtcaataaa ggtgaaggag tccaggaata
    13921 aatcccgcgc atacgcctca tagtcaaaat agagcgccac cgtctccggc acgtcagcca
    13981 gcaggccggt atcactggcg aactcaaccg caaaagcctc ctcactgtca gcctcgcccc
    14041 accaggcatc gcggaaggtg tcaaaatccg tctcaccggt atcctccacc cagagacgat
    14101 aagcctcttc gcagccttca tcccgtgcca ggcggaagcc ttcaacccag gcccagttga
    14161 tatggcattc agaggccata ttccccggga atccctcata atcctgaaac atcagttcag
    14221 gatcggcttc atcctggtga agagcacggc aggcggcgaa aaagtcgcgc tcatcatcaa
    14281 acgtggtcag gtcaaaccag cgtccggcga tgcttccaca gttgtatttg tgccaggttc
    14341 caacgtatac agcaggtgca acaacagaca ttcccgaatc ctccatagtc acatgttctg
    14401 cgacaccggg cggtgccgtg cctccataaa agccgggcga taaggcgggg atgcaggggc
    14461 gcaagggaag ccgcagcctg acgcacacgg ctgacgggag ttgcagggtg aggccgcgaa
    14521 acctgagcgg acgaacgacc ggaaaatcac ggcagacggg aaggagggac cgggtgaccg
    14581 cagcggcgaa gcgtaccctt gcatccctgc cagcccggct aagatggagg cacggcacag
    14641 cccgcacacg acgctgaggc cacagagctg aaaccccaca aggccatgcc ccgcatggcc
    14701 gggtaaaagc ggagccgccg gcgacttcct tattccggcg agtgcagagg acacgccgca
    14761 tgacctccag accacccctg atatgaaacg atcatgatcg gcgtgtccct gcgtaaaata
    14821 tacatgcctg gttcaccgga gaatatctgt gatatccaca agttttctgt cgcgcagcca
    14881 gtcctgcgct tcacgcacca cacaactgtc cggtttcacc gctttcagcg atgatgccag
    14941 tcgcttgatc aacatcacca gttcccgtgt ctgtatgtcc ggcattgtct ccggcggagc
    15001 agggggactc tcatttccga cgctcatccc atgcgcttcc agtgtggcac ggacaaacca
    15061 ggcgtcatcg accagttgct gtccaatcca ggccagatcg gcatttctgt cacgggctgc
    15121 cattcgctcg cccgacagcg ccttgctgat atggctgcgc accagattat ccaggagtgc
    15181 ctgcgcttct tccggtgaaa agccacctgg tatctctgtg cgcaacagcc ctttcctgta
    15241 cagaagctgc agcagatcaa cctgcgaccg cacagcaact atcagtacat aacggacaac
    15301 attcgcactg gcagtggttt ctgtatcatt cagcagttct tccggatcgt tccccccggt
    15361 gttgcagagc aacggcaaca gaggtcgcat ccacgcggga acctggtatt tttcgtctga
    15421 tttcatcata ttacgttctc cagcttgttc gggatcatcc actgctcaag tgcacgtacc
    15481 tgtaccggtg caccacgacc ggcctcaccg gcttcaaaaa tcagccgcca gagtggttcc
    15541 tcctgccaga aaaaccggtc aaatgatgac agtgatggaa aacgcccgca ccacccccag
    15601 aaaatcgttt ccagatcatg ctcctcaaca acaccagacc agcgggacaa ccagcttccg
    15661 accgtggcgg gagtatgaaa cgcaaggtca atctgcgcct gagcaaaaca tgtctggtat
    15721 tccagttccc gtcgttctgc ctcacgttcc cgctggcggc tgtatttctg ctctgtcagc
    15781 gtggttttct gacgacgacg ctcaccttca cgaaaaggca cggacgggaa cagacggtgc
    15841 cccgcatcgg atggcagggg aagaacacag acttccccaa aactttcaat cagtttatcc
    15901 acggcagcca gccacaggag acggttgcca tgaaattcct ccttcgtcag cgcaggggca
    15961 aaaaagcgga gatcggtcag cgaaatccgg cgactgccat tcaggcagcg aaagaaagcg
    16021 gtggcataag gccatgctga aagtgtgcgt ccctgacgac gacgttgttc tgtgacagcc
    16081 accgcctgca tggcggcctg ttgttctgcg ctgaggggga tcagtgcccg tgaacgtgcg
    16141 ctcatgcctg acctccttct gccatcagga tatggatgat gtcgttaaca ctgtatccct
    16201 gcgcatccag tgtcgcggca agcgccagcg tgtgactgat cgcggcaggc gtgaagatat
    16261 gttgcacctg aacgacggcg aaaggctgtc caccaccatt caccagaacc agcatccaga
    16321 gctgagagac gtctcccggc gaacataatg ccaggtggaa acgttcgtgg gctggcgtga
    16381 aacgaacctg gacggggaaa aagcccccga actcgctgcc atactctccg ttcatcatcc
    16441 agttagccgg tgcgtccact ttacgcatca cctcgtgagt gagattacgg cgcatatcgc
    16501 ttcccgcagc acggatttct tcatattcct gggcattcat ggactgcagg gcattcagtg
    16561 tcagttcggt attcatcatc gtatttctcc ctctgccgcc ccgtctccgg cggggcggtg
    16621 tggttgttca tcaggcggca tcagccaggt tatcggcgcg ttcagtggca tccgtctgtg
    16681 gacgtggtga acacatccag ccaggcaccc agcggttgtc tttcatatgg aactctgcat
    16741 gttcagcggc atcgcctttc ttcatcttct ctgcgtcccg tgcggcaccg gacagtccgg
    16801 catcattcag ggctgcgata atctgcggct ttttcaggtg tccgaagaag tttgctttcg
    16861 tcggctgcca ccagtcgcgc atgtgaaaac cgatggcgct ctccagtgag tcaagcggac
    16921 tgcgtgacgt gcgaccacac tcgcgggtct ggacaccgtt aaggctgcat gcggtgcaga
    16981 aactgagcag ggataacagc acctcctgac tgagtgacag gaacgtcgtc atgtcccgtg
    17041 accatccctc cggtaacagg gccgcaagac gggctttttc tgccatcagc gccaggaatg
    17101 cggcaccttc cttccctgac ggcgcatcgc tggtcagcga ataatgttta cattccaggc
    17161 tgatttgtgc tggtttactg tacgctccgc tgtcaaacac attcaggcag agcgtccatg
    17221 ccagcagtgc cagagatttg tccggttgct gcatcagtgc tgcctggact gccagcgtgc
    17281 gttcggaaga cattttcgtc agcaacggca gactgatttc ctccactgac gctttctcct
    17341 gcacctgttc cgtgcggtca gcgtcatcag tcgcgtcatc ttcactgcgc aactgcacac
    17401 cacgctggac acatacgttt ccataacgcc aggacaccac cacgccactt ccggcacgca
    17461 tctccggcgt ccacgccctg accttcgcca tgcagcgcat cagcttcatt tctgcttcca
    17521 gcaggtcgga ttcctcacac tggttttcca gcgcgtcgta acgcgtcatc agttcgttca
    17581 ggcgttcttc ttctgcctcc gtcagcaccg cttccggctc cggcagacag cggtatgttc
    17641 cggcatcctc acggcactca ccgacaggct ccatgcgtcc ggcgcaccat tcccagcctt
    17701 cggcttcccg aaggtgttca gcgacagcct ggagtttttc cagcagggca gcatcgagcg
    17761 ccacgcggtc gacataaccg tccccgtcat cgctgaacaa atcagtacgc agttcgtcag
    17821 gtgagaaagc atcagccccc acgaagcgga atttactgtt acctgccacc gccacttcac
    17881 tttcggtcac cagacgacga atggtctgta cttccggttt accgccccat cccgactggc
    17941 aggcggcttc aaacacctgc acctgacgcg cggtgtcgtt ctccagcgcc agcgcctgac
    18001 agtgctctgt ggtgatgcgg tcttctgcca gcgcatcgag gatgacaggc gcaaggtctg
    18061 ccagtttcag cattcgctga acgtggcggg gcgaatagcc cagcaaatca ccgatttgtg
    18121 caggcgtttt gccttcctgc gccatcgcac ggaatccggc aatctgttcg gcagggtgca
    18181 tatcccgacg atgaccgttt tcagtcattg atgcagccgt cgccagctcc tgcggaataa
    18241 ccttcacgcg tacaggccag tcagccggaa tgatgccacg ctctgccagc atgttgagtg
    18301 cagccagtcg gcgaccacct gcggcgacac cgtaacggtc accaggcagg gtatgcacga
    18361 ccagattctg cagcagtccg acgcccttaa tggactccgc cagttcgctg acggactccg
    18421 cagaatacgg caccgtgcgc acattcagcg gtgatttaat cagcgaggcc agcggcacgc
    18481 tcacttcctc cgtctgcgcc agcagggcag acaggacagt ttcctgcgtt tttacagatt
    18541 tacggcttgc tttgcgggtg gtttctggtt cacacttcgt tacaggcata atcatcccct
    18601 tataattgct tatgtcctgc cggacgttcg cgcgtccggc acctctctta cttaagaatc
    18661 ccgaaatccc tgatgtactg gttcatccag tacccggcac agtcctcaaa attccaggag
    18721 cgccagatca acgtgccatc ctgacggaca accaggcgaa aatgcgttcc ctgatcatcc
    18781 tcgataaaga cgttccggta ctgtgcggca acggcttccg cctgttcgcg ggtaaaggag
    18841 ccgtccggca gtccctgaag tattctgaaa tattcgctca tcctcatctc tccggtgatg
    18901 ccccgcgcag ggcggggcgg ttatcacagt caggccagtc agaacgggat atcgtctgaa
    18961 aacccgtaat cctccccctc cggcggttgc ggctgaggct ctggctgcgc ggccttacgt
    19021 ccacggcctt tcgttttcgc gccacctttt ttcgtcgcgt cagcactctg tggctgccca
    19081 ttctgctgag gcttcggttg cgcctgagcg ttctgctgtg gtgcacgtcc cagcatctgc
    19141 acggtgcccg tggtcttaac aagaatttca gtgacgtaac gggtgatacc gttatcttcc
    19201 cagctacggg tgcgaagctg accttcgatg tagacctgcg cacccttgcg cagatattca
    19261 cctgccactt ccgcgagctt gccgaacagc accacgcggt gccattccgt ctgctcccgc
    19321 atctcccccg tctgtttgtc acgccagctt tctgatgtgg ccacctgcag gtttgccact
    19381 gcgcccccgt tggggatgta acggacttcc ggatcattgc ccagacgccc gacgaggatg
    19441 accttgttga taccacgtgc actcataaga acctcctgac tgaaagatgt gagataaacc
    19501 ggataccggc gacgggacgt tcatgtgccc cttcagagga gcgaaagctc ctcctgccgt
    19561 ccacttcaca ggggtttctc cccgcagtgg acctttccga tgggggcccg tttaccgcct
    19621 cacgggacag ctgtccgggg gcggacgcaa aatttttagc cgggttcatg gctaaaaatt
    19681 ttgtggccgg tgcatctcac cctggacaga tggaacggga gggggttacg tctgcccccc
    19741 ggaaagtcca cagcgggaaa caggccgcag gggtggtggc aggagggaag actgctgcgc
    19801 catcaggcgc agtttcctca cacaccgtca ggtgtgcagg gagcgcaggt cacggcgcga
    19861 caggggcggc gcgaccggaa cggtcgctgc aagccgggtt ttcacgcatc ggaccgtagc
    19921 ggcagcccga aaccgcgcca gcgggttcgg cggagactgg cgggtgcgcc agcagctgcc
    19981 tgtcggagtc gggcctgacg accggatgcc gccaggcagc cgggagccga agtgggcgca
    20041 ataaaagaca gccagaaacg gtgacagtgt cacggaatat cacacctgtt ccctgcatac
    20101 agccatacac taccttctga ccggaaagcc gggccggcag cgccgcaggc gctgcacccc
    20161 gcatttccgg caggcgtcag cctgccaggg gttttcgcgc gggctgacag gcggcctcca
    20221 ccgcctgccc tgtatcacct gtttctgctg ccctgaagga tatagtgacc accgaggaaa
    20281 accgcctgct gattcgccac aaaatcgcag atttcatcaa caatctgcgc agtggtgagc
    20341 ggtttctgcc ccatttcacg acgccgcgca ttaatcacgg ctttgtaatt gcgcagaatt
    20401 tcgcgggttt ctgtgctgat attcatgaca tgtgctcccc gtccagcgcc tgtaacacaa
    20461 cgtccattgc ttcgcgttct tccctgacga acgcatcacc gtcgcccccg ttctcactcc
    20521 cgacggcata caggcgcttc atttcactgc ctgccagcgc caacactttt tccagcatca
    20581 ccgccgggac tgtcacctga cggctggcgc gcttttcttc catcagttca cgaacaacgc
    20641 cgtgaacaac gctgacatcc gcaccatatt caaaggcatc gtccagccgc tgcatgacgg
    20701 tttccagctc atcgtctgtc agactccagt cctgggctat gtggcgcaca tccccacggg
    20761 tcagcaccac ggcaaaaacg cattcactgt ccggcacgtc tgtatccagt acatcgcgga
    20821 taatttcttt aacagtacag tacatgttct cgtctccttt acaggggcgg cattgccgcc
    20881 ccgtccgtta atcagtccag tgctgcgtaa atcgcgttgc attcagggtg aaattcgatg
    20941 tgacgccaca actgcgcatc gcgcatccgg taaagctggg tcagtcctgc atccccgctg
    21001 tcgtggtaca gccacaactg gcggttaatc acaagggagg taaggatgat gcctgcggca
    21061 tcagcactca cggtacggtc aaaccagttt gcaccgttca ccatgtgaaa acggtcgcca
    21121 tccggcatca tgtaaccgcc accgtccggc agtcggatga agtgccagaa tccgccttca
    21181 tattccggca tcatttgttt cgccagcgcg tatacagacg cttcggcata cagaaaatcc
    21241 tggccaaaca ggtaaggcag aaaagacaga cggtcgcact cttcaacgtt cagagtgaca
    21301 ggtttcgcat attgcatgat gttttcctcc gttgttcaca tattccgcga caccgggcgg
    21361 tgccgtgcct ccataaaagc cgggcgataa ggtggggatg cacagggcgc aagggaagcc
    21421 gcagcctgac gcacacggct ggcgggagtt ggagggtgag accgcgaaat ctgagcgggt
    21481 gaacgaccgg aaaatcacgg cagacgggaa ggagggaccg ggtgaccgca gcggcgaagc
    21541 gtacccttgc atccctgcca gcccggctaa gatggaggca cggcacagcc cgcacacgac
    21601 acggcggcca cataactgaa aaaccacaag gccatgccct gcatggccgg gtaaagcgga
    21661 gccggcggcg acgtcctttg tgctctgtcc ggggaccggc aggaaaaagg cggggattta
    21721 ccccgccagt gtcatcattt ccggattttt gccagggcat ccgccatgct gcggatttcg
    21781 ttccgggctt cccggaccac ctcccgcaga acaccacggt caaaccagcg gcggggcggt
    21841 tcatccggta tacgcagcac atcccaggcc agccaactgg acaggatatg ttcaatacaa
    21901 tcgctgacca gatacatttc caccgccgaa aaacgtgcgc cccggttcag gatggcgtcg
    21961 agcaccacgg gcaggttttc gtcgtcctca tcgagccagc aggggtgatc gctctccttc
    22021 acgaccaggc gaaagcggcg cgtctggcgg gtcagtaacg tctgaccggt ggtggtcagc
    22081 gccgcccgga tacgccgcgt atcttctgcc acctggtaac tgacacagcg tactgcatcg
    22141 cgtaccgcat caagaactgc cctgatttcg tcctctgcca gccactcacc ggcatacggg
    22201 aaagacacct gcgttccgtc tgccggaagt accggcagcg actgccactg actgccggac
    22261 agtacaagtt ccgccccggt tccggtcagt ttgtgaatgg cggaggccag gagcggcggc
    22321 agtgcgccgc cgtgacggtt gataagtaac gtcgccagcc ggggcgcatc cagcagaaca
    22381 taaatgctga tccagcccgg atggttcggg ttccgctggt caacatccgt ctcttccggc
    22441 cagccctgcg ccagcagcag gttgcgggcg tgacgacaaa tcagtgcgtt cagtgttgtg
    22501 ttttcgttca tgattaatcg ttctccctga atcgccgccc ggaggcggcg ataacatcag
    22561 tcgttttcgt gtctgttgtt tttcagcagg gtgcgggcct gattaatcac cgcctgacgg
    22621 cgggtgacgc attccgggac ggcgaggcca tgatcccggg cggcccattc acgtttccac
    22681 aatgcctgtt cagcggtctg aatcagggac gccagcagtt cagcagggac ggtcacctgg
    22741 cggttttcgc tcacgttatt gatgatctcc atcgccacgc cggaagaaat accggattca
    22801 gtccgctggt cttccgggat gtcctccagg cgcgccagga cggttctgat ttcatgatcg
    22861 gacagggaaa tatccattcc gtcagcgagg gcctgaatct cttccgggga ccagataacc
    22921 agcatcagcg gcatgtctcc cggatacttt actgccagct cccggcatag cgtttcgcat
    22981 gttccgtaca ttctcgtctc cttctcaatc tttaaagcac gcttccagtt ccagaatttc
    23041 ggtatatgcc tccatcagga ggcggtttgc gcgaaacagc gtctgacgca ccagacgttc
    23101 cggcagggtg tcggcattaa agctgtaccg ctgctccatc tcattcatgg ttgtacgcag
    23161 gtcgcacatg gcattggcca cctccgccac ttcggcgcgc agtttttcgt gtcctgcata
    23221 gttcatttac gcagcctccg gcataaacca gtcatcattc gcggccccct gctgcatggc
    23281 ccgttgcacg gcggcaaggc gttgctgccc ggcacggtga tactgctcaa gcagctcgat
    23341 accgatatac cggcgtccgg actggagggc ggcgacgcag gttgagccgc tgcctgcaaa
    23401 cgggtccagc acaattgcgt tcgggtgtgt gaagttctca atcagcggtt gcaggctggt
    23461 aacgggcttt tccgtcgggt gatggcgatt gcccgaatat ttccagccca gcacgtccgg
    23521 cagcgggttt tgtggcagac gtggacggcc ttttgccagg atgtaggcgc attcgtggcg
    23581 atagcccaca tatgcggcct tcgatgtgta gtttttggtg aagaccaggt gaccaacaac
    23641 gctgaatccc gcatttttcc aggcggccat aaagcgatcg acgcggttcc agccgtagaa
    23701 gctcaccatt aacgcgtctt ttttcagtac gcggaacatt tcattacagg ccggttgcag
    23761 ccactcatcg gttttatcgc cggcgatggt gcgcccggaa cggtcacgga aaccgacgag
    23821 atatggcggg tcggtgagga tgaaatcaat ggcgttatca gggatacggg ccataacatc
    23881 gatgcagtta ccgaggacaa aacgggacat accaaactcc ttctcttaca gagggcaccg
    23941 ggagggcacc cctgtggtgc ccgttccccg ctgcctgtgg gctgttcacc gcgtccggta
    24001 acgggtcagg ccgaccgtgg aataccgcag ccgtcgtctt tctgacggca aggttatgcc
    24061 gcgaaagcgg cttgaagggc gttcatcaga aaagggcgac aggctggcgt acactgtcac
    24121 cgacgccagc cgggagcggt aaaagcgccc gtcccgtgcc cggccggacg cgggacactg
    24181 accacaggca gcgtttatgc cgcgccgcgt cagccggcgc agtaaaaaga ggagcacggc
    24241 gacatccttt accggaaaag aaacgggcgg tagcccgccc gtctccgtca tacttcatcc
    24301 gccataatgc gccaccttgt cgactatcca cgcaggtccg gtgacttccg gcagctcatc
    24361 gtcatctgtc ggggaagacc attccagctc cccccacagc acatcaacga gctctccgcg
    24421 ctcatacaac tgccagccac agaaatcaca gccctgttcg gcataccagt gctccagcgt
    24481 gcagctgaaa cggcggctta attctgcaat aacgtcgctt tccggctgac accacggcgt
    24541 gtcgaaatca acctgaatga agttgtctcc ctgactgctg atattcaggt catacccaca
    24601 aggccacttc acgccatact gttcggtata ccagtgatat gcagaaggaa caccgttcag
    24661 aacaccaccg ttaaagccat tcacttcaac atcaaggcgg gtgggcagaa ccgtcagcag
    24721 gtcaaacggc atggtttttt ccggcagtac gttgtcacac agacggttcc accacaccga
    24781 tacatcctcg ttactccaga agccacacca gtcacctctt tttgcggtga aaagcgcggt
    24841 gatggtatcc ctgacatcgt ccggtaatcc ttcccagcga cgctggccag taccgctctg
    24901 cagccagagt tcatgcagca tcaggcagtt ttgctcatcc agcagtacac cgttctgcag
    24961 gtgtgtcagc cagcgggtga acgcgatatt ctccggcgat acaacgccac gtccggcagc
    25021 cgtcagtccg gggcacggtt caaaccacac atcttcagtg gtctgcagaa gtccggcact
    25081 tccggccaga aacagctgaa taccttcatt tgtggcgcgg cgataaaacg gtgtgacggc
    25141 accactggcc agtcgtttaa tctcagcaat ctgtgccggt tcaccagaaa aatgcatacg
    25201 gttactgcac cagtttggca tgggaattct cctctcacat gcggcctcca tcaggccgtg
    25261 ccgcacctgt aaccgggcga ccgcatacga atgcaggtgc ggccccgaag ccgcagcgcg
    25321 tggaggagga acgacgaaac gtgaccgggt gaggaggccg gcgacagcga cacacttgca
    25381 tcggatgcag cccggttaac gtgccggcac ggcctgggta accaggtatt ttgtccacag
    25441 aaccgtgcac aaaatgctgt ggataagctg gacacagcac tccacagcag gcatacagcc
    25501 ataccctcag agaattgagc gttttttagt cagaacaatg acatttgact gtgtgcggat
    25561 tcagttatta gaatactgta tatatataca gcaaaagaaa aggaagatga gaacatgatc
    25621 cgcattgaaa ttcttttcga ccgccagagc acaaaaaaac tcaaatcagg cacacttcag
    25681 gcgctacaga atgaaatcga acaacgtctg aaaccacact acccggaaat ctggctgcat
    25741 atgtgggaat ccccgtcctt cagagtgagg agctgtcaac cagcactcca ttgactcaaa
    25801 tgacgggaaa cgcggaaatc taaaaattaa gccgcacacc ccgagaaatt ttgcaggttt
    25861 cacggagcga gactttttta gatcaggttt tgcatcttca ccctagccgc tttcagacgg
    25921 gcctgttcac cgtcatcctg agagtgttct ggtgaaactg tactctgaag ccctcccaac
    25981 gccttcagta cctcttttaa atcaccggag gagggtttcc agcctgttat cctggcaatc
    26041 tgactaatga cctcatccgc attcaattct ctggtgaata gcgcggttaa cacggcggga
    26101 attcgctcat caagctgatg cattatcagg ccggttatca aagcctgccg atataactcc
    26161 ccccgttttt tccggggaac agtttcgatt tcagccagtg caagacaatc ggcagagtcc
    26221 tccgggtgaa ggtagagtga aagttttttc ctttcttcgc cttcgtacat cgattactcc
    26281 tccgctttga agcgtgcaat agcctggacc agcgctgtct gaggttcatc cataagaacc
    26341 accttttcgc caaggtggtt ccatgcctta cgaacagcat cagcaatgag tgcagccccg
    26401 ccaccgacaa tatatacgcg gtttacgttt cgatactgca gcaaatcatc aacaactagc
    26461 tcgcccagtt tatttatcgc tgtatcaatg gtatccagca ccagatttag tttagagacc
    26521 tcatttacaa cctgccccac aaaatccagg ttattcctgt ttttgatcaa ctcgtcagca
    26581 atcattggac tggtatcact gctggccatt ttcagtgcag tgagcgtggc tttcgtgacc
    26641 attgatacac caatatcagg attaccatgt acagccgaaa catcctcaaa ctggccaaca
    26701 ataaccccga catccagagt tgtaccaccg agatcgataa ccaatgattt ttcgtactga
    26761 ccaacgttgt cagcaactag gcgagtaaat acagccggga gtgattcagg catcacttca
    26821 acgcttttaa tggtaaaagt tccccctttg ttcagggtga cttctcgcat caggttgcga
    26881 atttttctct caatgttaag cgtatttttc tggcagtcgg ccgtgtaaaa ctcgctgata
    26941 ggtaacgtaa cggtgagatc gatctcctgg ggttcaatgc cactgttcag taatgcgtgg
    27001 tgaactgcca acacatttgt atcagagtac tgatactcaa tatgagtcgt gctgatcgca
    27061 tcttcgctga ccgggtcgta ggtatattta cggccatcaa gcagatagtt ataagtacga
    27121 ctgctgccta agccctcgac tttccagtta tggcgaaaag agttcactga taccgcactt
    27181 ttcagcgtct tgccttcaaa ccaggcaagt ttcacatttg tagaaccatc atcgcaaaaa
    27241 attctcatac catctccatt tatactcaac ttgagtattc atctttacac aataactact
    27301 cacattaagt aaatacgctc ataaaccaca ggtaagtact caagctgcgt acttcacttg
    27361 ggcagttaac tactcaattt gagcattata tcatttatct tgcagcaatc tagcatttat
    27421 ctagcataaa tcttgctgca agattgcaca caaaaaaaga atgcagcaag attgctgcat
    27481 cccggattaa tagccctgtt tttccttata tgcctcaact gctttttcca gaacttccac
    27541 cagtttcaac ccctcacgct ctgcgatatc ccggatctct tggtcgaaag caggtgtgac
    27601 acgtgttgca aatgccagcg tcctgtttgt ttttctggca gaacggccat cgcggcgggt
    27661 atatacctcg gtcactgttt gcgtaaccac aggctcatgg tgcactggcg atgagtctgc
    27721 ctgttgtctc tgaacatcag tggcagcgac agccggagct atctcaggcg cattcaagct
    27781 ggtagccact tcgtcggggc ccgggggtac ccccaaccca ctacgggttc ttttagttct
    27841 aagttttgac gcatcagcca ttactcttct ccaatcttat cgatgagtgc ctggataagt
    27901 tcatcagctc tctcgtttaa acctttataa cgtgtttctg tcacagcgag tcctgaattc
    27961 atcgcttttc tgtatgctgg cttctcaaaa aggcatccag ataaagtttc ataacctgct
    28021 tcactgatat atgcacgagc atccagctct tccgcttcgg tacctacccg gcttaaagcg
    28081 aagaccagtt ttgaacgtgg tataccttct tttaccaacg cgttgaaaac tttaattgcc
    28141 gggacaagat cgtcaaggga ggctcccgtt ggctggacga taaggtcggc gaccttagca
    28201 atttctgaag tacctttact tgcacgagca gggccatcaa gaatcagaag gtcaaaatcg
    28261 ccggcagatt ctatcgcctg ctttgctgtt gaaaacactt caacggaagc aaccggtgac
    28321 aaaccagctg acagacgtct acgatgccac tcagcactcg ttgcctgttc caagtcgaga
    28381 tcagctaatt ttgttttgat accacaggcg gatgcttcac gcgctaacgc tctcgcttta
    28441 gtgcttttgc ccacaccacc tttctgagaa ataaaaccaa ctatccaggc catttagccc
    28501 cctttacttg ctgctatctt gcagcaaaca tcaatataaa ccagattgag attaggtaga
    28561 gtaactcttc cagaacaaga taacagcttg cagcaagatt gcaacaataa ataatcttgc
    28621 tacttagcag agtgctggaa ttgacagccg aatacttgtg tcacgttaac aataaaagaa
    28681 acctcattca gaatcgcatc gacagatgaa gcaagattgc cgcaagattg cagatagcat
    28741 aacataaaca gagcgctagg ccgtatgtgt acagcatgat agctgcaatc tattttattg
    28801 ctgctatcat gctgaaatat cattattaga atcaggcagc cctcgccgca gccatgtaac
    28861 tgtgcgtagc gagcgaactg gcgaggaagc gttccggcag atggctctta cgctcggcac
    28921 aagaagagtt tcccatccag gcaagtacta tgggttgtca gcgccagaga tataagactg
    28981 ttctggcctg agaacgtggc ctctatgagt ttggtgctct atcttccata gatgaatgca
    29041 ccagctcccc cttgactgaa ccgcgaatac ggctttttaa cgcctctcaa gttgttttat
    29101 cagcgctacc gcatcggctt ccggcatggc gaactgcaca cgatgacgcg ccgcgacatc
    29161 cagcgcaaag accttcgtgt agacctccgt tgagctgatg gacttatgcc ccatcagact
    29221 ctgcaggacc ttcagcggta taccggcata cagcatgtgc atcgcatagg aatggcggaa
    29281 cgtatgtggc gtgaccggga cagagaacgt cactccatca gcggcagcgg cggcaaccgc
    29341 ctccccaatc caggtcctga ccgttctgtc cgtcacttcc cagatccgcg ctttctctgt
    29401 cctgcctgtt cgtttattac gccgttccat ggggattttc agtgtggcca ccatcgtctg
    29461 cagctggctg acgtaccagg cgtcagagag cggaaccagc cggtgagtct gctgaccggc
    29521 gggcattctt cctgccgtcc tggcggcttt ttcggtccgc tgcttcagag tggccaactg
    29581 cacaaacgga tacggaggcg caagcgaaaa atccccccgc gtcagcgcca gtgcttcatt
    29641 aatgcgcgca ccggtgttcc acagtgtggc cagcagcatt ttgcggcgca gatccggcac
    29701 gtaatggagc agggcgctca cttcaggggc cagcaggtat tttggcagtt catcatgaac
    29761 catcgacatc tggcggagtg ccagcgctgc cggataatcg atggcaacag gaagctgagc
    29821 agcatcccgg gcatggtgga tcggtgccgg caggtgctgc atatcagtgt tcactccacg
    29881 gggaaacgtc gggcaggttt tccacaacac ggtactttcc cggataacgg agctggttac
    29941 ctccacgcgt ccagacggtg acaacaaaac cccctccctg gttgttttcg gggagcaggc
    30001 gatcgcactg ctcactcagc atgtggatca caaacggcgc atcgctgccg tcttcaaata
    30061 ccagttccag cgcatcgcgg ccatgcagtg ctcccttact gatgatgacg tactcagccc
    30121 cccgcatctc cctgagtaaa tgttttgccg catccgggac aagcagcctg gctgcgccgg
    30181 cattccagct gaggtagaca tatccggcct gcgcctgtac agactgccag taatccgttc
    30241 cggcgacagc ctgaccttca ttctcaataa aaaacatggg tgactccttt tttcttttcc
    30301 gggcagggac ggtcatgcga taacgtcatc catcatcact gcccttaccg tcacgccgtc
    30361 tgataacatc atgccgttat catcatcacc atgataacaa gcatccatat aaccacgccg
    30421 ttaaattacc ggagagccgt ttccgtcaga tccgactttc cggaatatca caactttccg
    30481 gaagtgtacc acgctgagaa aatcatcata tcgctgtttt ccgcccctgc gcggcgcatc
    30541 acaggtcagg gagatgacac aacatatagt gtcatcagca gctgaattca gcactacatg
    30601 ttgtgctgac gggagatggc gctgtgcgga gggagaggaa gatccgtaac agccggaaac
    30661 cggatctgtg cggcagagaa cgggcaggag atatgataat taagcagcac acataaaatc
    30721 atatctgata tgattttcac cattaaatat aaaaccatac ctgatattat cataaatcaa
    30781 taacacacaa atcatgcagg atgtgatttt tacctttaag aacattatca gatggagcgt
    30841 gcaggatgga tgaagaaagg gtgttttcac tcagctatga acaactaacc cggtttacgg
    30901 aacgacgcat ccgggaatgc aatctggaca gccagggcgc gatttatctc tgcgagtcag
    30961 ccaaagccgg agccgtgctg atcttctggc atgaactggc cataaatggc tatgcctcaa
    31021 tgaatgccat aaagagacag gagctcattg acgccgactt tcagcgcctc agaaagctca
    31081 tctggccgga ggatgactgg aaatgactca gagccgccga ccgtcgccac tgcagcggag
    31141 agtgctgatt gtgctggctg cccttgatga aaaacggcca ggacccgtgc tgacgcggga
    31201 catagaacgg gtgctggagc gaagcgggga agccccggtc tacgggccaa acctgcgcgc
    31261 ctcctgccgt cgtctggagg atgccggctg gctgcgtacc ctgcgcgctc cgaacctgca
    31321 gctggcagtg gagctgacgg acgccggccg ggccgtggca cagcctctgc tgctggcaga
    31381 acaggaccgc ctccgggcag aacagcgtgc ggcagaagtc gtcgtactgc cgctggtccc
    31441 ggcagcaggt ctgcccgcag atggcacttc tgccactgat ctggaggtgg agctgaacgg
    31501 cataacgtat caggcctgcc gtggggattt tgtggtgcgt ctcgacggca gcacctgtct
    31561 gcagttatgg aataaagaag gcagagtggt acgccgggag ggcgatccgc tggaagtggc
    31621 gcagtggctg caggcctgtc atgatgccgg aatagaagtt cgtgtacagg ttaatgaaag
    31681 cgtcactccg taaaaacatg ccagcacggt ttacacatca ccccatggtg gcagccggtt
    31741 aactttcctg cgccaccagt ggaaagaggc cgtgagcagt ccgaacaaaa aaccacccgt
    31801 gatacttata attatggcct cacctgccac cattcctgac ggcccccggt agataaacca
    31861 cattgcacat ccccaggaaa tgccccataa gctccccatc aacagcgtga cctgccagaa
    31921 cggcataaag ggtaatggcg gaagccggat acccagccgc cagagaatac acagaagagg
    31981 aggggcataa ttgctccgcc acatcttttt gctgtccatc agggcaatgg cccgggcttt
    32041 tttctgctca aaggtcacag gagcacctcc gttccatgat gtaaacgtga cacagcttat
    32101 tgttattgat tattattatc atgctgtgat tatgttatca cgcaccggta cacgataaca
    32161 ataaaggaga aaacagatga aactggatat atctgtaaag tatttactga agagcctgat
    32221 accaagcctc attattctta cagtttttta tctgggatgg aaagatagcc aggaaaatgc
    32281 aagaatgttt tatgcgttca tcggatgcat tatcagtgcc attacttttc ctttttcaat
    32341 gaggataata cagaaaatgg taataaggtt tacagggaaa gaattctggc aaaaagactt
    32401 ctttacaaat ccagttggcg gaagcttaac tgcaatattt gaattattct gtttcgttat
    32461 atcagttcct gtggttgcca tttacttaat ttttatattc tgcaaagccc tttcaggaaa
    32521 atgaaagggc atcattatgt gtcagatacc aataagttta ttgacctgct caataaactt
    32581 atcattaaca aacgcactca cagccgccat aataattgca aaacccagaa tacctacagg
    32641 ggttcccagc atgacggaaa acgtccatgc tgtaactgct gaagcagctc ttcctgctgc
    32701 cagtgattca agttttacaa aaaatggacg ccagttgtcc gtttttacag ccttttgtaa
    32761 ctccacagca acatcataac gatcaataac ttttccggta aaaccaaatg ccttactgaa
    32821 cagcttaaaa ttctccgcca tatggacctg attaatagct tccagggctt tagaaatggc
    32881 catgcgatct tgtatgctat atttcttgtt cagattatta cggaatttat caaaagcgtt
    32941 cagtgcatca tccacactct taatctgttt cccttgagat acagaagcca gctccttagc
    33001 aagttcggat gcttgtttac cgaactcatc atatatcgtt ctgtagaaat cagaggtgag
    33061 ttttatggca tcctttatca tatttatttc atcccttttt cttttctctt cagctatttt
    33121 ctgatttatg tcagcaagct ggctacggat attctctttt tccttcaaca gggcgttaag
    33181 agcatcattt gcatgctttt gttcatttgt tctggcactg acgttatttc tcgccgaatt
    33241 taccgcagat tcagcagagg tgattttatt tctggcatca agcaatcgtt gccgtaactt
    33301 atcccattca gcaacttcag cattataaat tttcttgtca ccttccacat cctctgtcac
    33361 caccggattc cggtcattca gaatattgcg tttatagtcc agaccggaat gggtcagaag
    33421 ataattcagg tttgcacggt tatcgatgac cgcgctggtt gtgattttta tcgtaccggc
    33481 aaatctcgga tcaccactca caacaataga atgatttgtt gatgaacgcc ccgggaattt
    33541 ttcaggggaa actatcgttt tgccgtcagg cgtgtttttc tgttgcgtca gctgttgttc
    33601 agctctggtt aatgcattac gggccgtctt aagcgattcc gtcaccgaac tgagtttatt
    33661 ccgggcctct gaaatggccc tgtcattccg tgtctgctgc ccgagcagac gggcctgttg
    33721 tgcatcaaga acggcaaggg ctgccccgcc atttttttcc agctctgaca accgcgtgtc
    33781 cagaatacct tttctgcgct cgtattcagc aagcatatca gcggccttct tgtctgcgtt
    33841 ctgcgcatcc ttctgccgtt tttcagcctg ttcgattaac gacctggcct cgcggggaga
    33901 tatacgaaga gaatcagcat cagcaaacag catccggctc tctgtatgca gccggagggc
    33961 atcatatccg gcgatttcag tacggaatcc gtattctctc agctctgcct gcaggagtct
    34021 gaactcctgt cgtgtaatat cagcgacatc tgcgtttccg gcacggagtg ttttcagtgt
    34081 gttttcatct ttttcccttg cagcaattgc cgcagaaagg cgttttccgg cttcagtacg
    34141 cttattctca ttctctttct gctgcacgct gagcgtgttt ttgtacgctg tgatttcctt
    34201 gtcccttttt tcgatatcac ttcgggttgg ggaatcatcg acccactcgt ttccgcccca
    34261 gatggttttg ttcccgaagg aactccatgc aggcggggta ccatgaaaaa catcgacacg
    34321 tggagggttt acataaatat caacggccat aatttcatgg ccatctgaat cataccccag
    34381 cgattctgca ccgggatttg taatacgtac agggtcagac attcggtatc tccttcatcc
    34441 tctgaggaaa acaaatgaat taactgctta cgtatatgga tacatataca gtattaattt
    34501 attaaggaga aaatggcatg tcaagattta atgatagtga ttatcattaa catgaagtgg
    34561 tgttaaaccg ttcagaaagc ctgctgacgt tgtggcagca tcacagtctg ggtattctct
    34621 ggtgcaatac aaaaaccgga ctttcccgta acaacagaca atgttacggg aaaagttaca
    34681 gaactgagcc acaaaatatc tcagtacctg agatgcataa aaacaccgta catatgtttc
    34741 tcaaaagggg gatgatcgtt atccagccat ttcggtggat cggtatgcag aacaccaatc
    34801 tgcttcatgc cggtatgctg cctggatttt ctcgcgcagt aatcactgtc ttcatatccc
    34861 atcgtttcga tgaccactct ggccgtcttt ccgtcaggag cccttgctgt aacaatgaaa
    34921 tccggcagta catatttctt ttcaccatta acataaactt ctgtatcaaa caggggcctt
    34981 tccagactta attccggcgt acctggcttt cttttaagcc agctggccac attcagaaga
    35041 gcagtgagcg tgtttcgttc cagtttgctg tccacaggca ccggacatgt cagttgataa
    35101 agagcatgag catacccttc actgcagatg attctgccat cggcactccg gcaaaaagca
    35161 agaataaccc agtaaggggg acgtattccg ccctgcgctt caccattaat actgatcccc
    35221 cgcacaggtc tgatgacctg aatattaccg ttcgcccagt gaatttccac agcatcacga
    35281 cagatgtgct gcgccataaa aatctgaaag aacatatgct ttcttctggc aggccagtgt
    35341 cgttctgaat tctccagcgt ttccattaac cgctcctgag acctttcaga cattccaggc
    35401 cggaaatgga taatttcaga taaggatctt ccccgaatat actcctgctg cagtgtaacc
    35461 tgccgtatgg catcaagaca ctcacgataa ttagtccgga tacggggatt agccagggaa
    35521 tccagtttat tcagcccagc atcgtcaata agagacagga gtaaacgggc aaggcgcaga
    35581 cgtttcttgc gaacggagga gctctcacca ttatggtaag cagagcgggc ctgcagtctg
    35641 ataacagaat tactgtcatc aggtggtaaa aagtttctgt aatttatacg ggtgctaacg
    35701 ggatgccggc ttgctccgga agatgtttcc tcttcatttc cggataactg acgatacatg
    35761 gggcattcag gtgcatgctc atgattaaaa ctggacagat aaagagtacc cgtattgttt
    35821 ttaagagcag caaaattcat ggccggcgaa tctcccggaa tacagtcaca ctgtaaccat
    35881 gcatcatact cacgcatttc ccggagaata tcattggcca ggttatgtag tgccatggtt
    35941 gctggctttt tcagtccaca gaccgtcttt actgcagtct cttcctcact gctaagctcc
    36001 cgaatcactt tgccccgggg attcttcttt acaagtatca ttggcataat gaaagcactc
    36061 cttatgtaca tgcttaaaca gtaagtaaaa tatacaaaga acaccccatg aaacaaaggc
    36121 agagatgaac tgtgcgcccc gatatcttct tctgatatcc tttcaaatga attgttttta
    36181 tttatatcat gacaacatta aagtgagaga atatatcaac catgtttctg tcagggttca
    36241 gtagaatatc aattcgactg tcacgccata actgttacta cctcgagtat aaatattcag
    36301 agaacggact tattagccat gaaaaatcac ctgatgggaa atttactaat ttatgaattg
    36361 tgatattatt aaagagtaaa accagccagc cgaggtattt atgggaaaaa atcaggaaga
    36421 aaggaaaact cccgtcattg tggtgaaaaa acgtcgtact ttttcgctac cgtccttatc
    36481 cgaaaaaaca gatattatcg cacctgtatt tactgaacag acggcagaat cagcccccgc
    36541 cggaataaat tcatctgccg tcgaaacaca tatccccgaa gcgccagcca ggaagaagaa
    36601 aaagaaaaga caccgttttc cacgtccctc ccactggacc cgtgagtata ctcacgagtg
    36661 cgtggaaaaa ataaaggccc tgtttcctca tcttcgtgct gaaggtggtg gatttattcc
    36721 cctgaaaatt ggcataaaca atgatatttc ggcatttctg gcagaacacc cggagacaga
    36781 actgaccatg gacgaatggc tctgtgcagt gtcctgcatc acatcccgcc gcgtatatct
    36841 gcagcggaca gctgtcgccg gcgtcccacg ttatggcctg gatggtcacc ccaaagggca
    36901 ggtctcagac tctgaagcac agagtgccgg tcgcagactg gcgacattag aacaaaagtg
    36961 gctcaggatg caggcacagc aggagaatat ttctgggcaa taaacactcc tgataacagg
    37021 tggacattac gtatcagaaa aaatgggaag agaatgttat gtgtgattca gaagcggaga
    37081 agattttatt catcctgaat aaacatgaac gggcatacag acgcctgatc cccgtatata
    37141 tttttttagc tatagtatta tggattttac ctattctcct gattgtatac ctgtatttct
    37201 gtatagttga caatgagttt ctcagaatcg gtttctgtat tgcatggaca gcatactact
    37261 ttctttcgct tttcaagtat tttgtaaaat acttctttcc tctcgatcgt gaagatttgc
    37321 ttaaactgat ggagctcgcc aaagattctc ctgatgcgaa acaggagctt cttgagcgtc
    37381 ttttgtccgg aaaaagaatg accgggcggg atgaaatgga tatcaagcgc attctaaaaa
    37441 caaaggaagc acttgataac atcaggaatt ttattgataa acagcagtta acgaatagtg
    37501 atactccgaa gaaaagcacg gtcccaaatg aagataaaaa aggcataaga tgatgatcac
    37561 aggaaatgga ataaacactg taactgtcaa tggaaaagtg aaacacatca cagaactgga
    37621 tgatataaca ctttgtttag agtggacaaa actacgagag gaaaacaatc gtctttatga
    37681 aattaataac caggcaaaca gaggatggcg tggtttcatc ctaagattga taggggttaa
    37741 cctgccagat aaaaggactg agtttacaca acgtctctta ttgacgcgca aaatttcggg
    37801 ttcggtcatg aaaaagtaac aaaaccgcgc aacagacata aatatgacag tagccatgaa
    37861 aaagcagaga gagacagggt gatacagaaa agtaactatt ttttagctat agtattattg
    37921 gttttaccta tttttgtgat tttatttctg tatatttgac aatgagtctc tcagaatcgg
    37981 tttctcgaag tgacgagcga tcagaatgga gacaaataaa ttcgggaaac gaaccttcac
    38041 ggtaagtttt gtcacattta tcatccctaa acatgagaat tatcaccaaa actaaccacc
    38101 caaccaacag cagcgccggg agcagcatta taaaatcatg tacagagttg cgtgacgaca
    38161 tgataagttc tcattctcaa tatgttcttt accagattat accagtgata ctttaacgca
    38221 ccatcattaa tgctaatgta tgcatacatt agcattaatg atgaaggaga agaacaacaa
    38281 tggcagtcaa cccacttatt ttttggattt cttaattttc gtctgataaa gccgctttac
    38341 ccgttcaatt tctgccagcg ttgcggacca gtgtagttcg gcggcatgac gatatctggc
    38401 agccattttt tccaccatct ccggattgct gtccgcgctg cggtgctgac gccccttctc
    38461 cccaaaaact tcaacaacca gccactggct tccggttctg ccgaacaatg ccttgcacaa
    38521 acgtctcaca gtcagcgggg ttaaatctgg atcgggatcg taggcaatgg tagcaaaaat
    38581 aaagcgcagc atttctgatg tcttgtaacg ctttacgctg gcagccaggc gtgaggcacg
    38641 ttgcggtgct tcccagcggt cgttactgta attagccact tcagccaggg gtcgtctcag
    38701 aaaacggaaa ataaagcacg ctaagccggt tgcagaggcc gtagcggcct gaacttcccc
    38761 gcgccgatct tggcgctgct gcgccatagg taatcaccgg tcaggttgat gtgctcccag
    38821 ccgagtggcg acaggtactg caatagcgag tcatcgacgg catgaccatt gccgcgcaac
    38881 gcatgcgccg cacgctccag gtagaccgtg ttccacagca cgatggccgc cgtcaccagg
    38941 ttgaggccgc tggcccggta gcgctgctgc tcgaaactgc ggtcacggat ttcaccaagg
    39001 cggttgaaga acacggcacg ggccagcgca ttgcgcgcct cgcccttgtt cagcccggca
    39061 tgcacgcggc ggcgtagctc gacgctttgc agccagtcga ggatgaacag cgtgcgctcg
    39121 atgcggccca actcgcgcag cgcgacggcc aagccgttct ggcgcgggta gctgccgagt
    39181 ttcctgagca tcagcgaggc cgtcaccgtg ccctgcttga tcgaggtggc cagccgcagg
    39241 atttcgtccc aatgggcgcg gacgtgcttg atgttgagcg tgccgccgat catcggcttg
    39301 agcgcgtcat aggcggcatc gcccttcggg atgtagagct tggtgtcgcc caggtcgcgg
    39361 atgcgcggcg cgaagcggaa gcccaagagg tgcatcaggg cgaagacgtg atcggtgaag
    39421 cccgccgtgt cggtgtagtg ctcctcgatc cgcaggtcgg attcgtggta cagcaggccg
    39481 tcgagcacgt aggttgagtc gcgcaggccg acattgacca ccttggtgtg gaatggcgcg
    39541 tattggtcgg agatgtgggt gtagaaagtc cgtcctgggc tgctgccata ttttgggttg
    39601 atgtgccccg tgctctttgc cttgctagcg gttcggaaat tctgtccgtc cgatgatgat
    39661 gtggtgccat cgccccagtg cccggcaaag ggatgccgaa actgagcgtt gaccagttca
    39721 gccaacgctg tcgagtacgt ttcgtcgcgg gtatgccagg cttgcagcca agcgagcttc
    39781 gcgtaggtcg tgccggggca ggactcggcc atcttggtca ggcccaggtt gatcgcgtcg
    39841 gccaggatcg tggtcaacaa caggttcttg tccttggcca gatcgcccga tttcaagtgc
    39901 gtgaagtgcc gggtgaagcc cgtccactca tcgacttcga gcagcagttc ggtgatcttg
    39961 acgtgcggca ggaccatggc tgtctggtct atcagcgcct gcgcggtgtc gggcaccgcc
    40021 gcatccagcg gcgtgatctt caagcccgac tcggtgatga tggcatccgg caggtcgttg
    40081 gctgccgcca tgcggttgac ggtggcaagt tgtgcttcca gcagcgtcag ccgctcatgc
    40141 agatattgtt cgcagtcggt ggccacggcc agcggcaatt cgctggactg cttgaggctg
    40201 gtgaacttct cgggcggtac caggtagtcc tcgaagtcct tgaactggcg tgaaccctgc
    40261 acccagatgt cgcccgagcg cagggagttc ttcaactcgg acagcgcgca cagttcgtag
    40321 tagcgccggt cgatgccggc gtcggtcatc accagtttct gccagcgcgg cttgatgaag
    40381 ccggtcggtg catcggctgg cagcttgcgg gcgttgtcgg tgttcatgcc gcgcagcacc
    40441 tcaatggcat caagcacgtt tttggcggcg ggcgcggccc gcagcttgag cacggcaagg
    40501 aattccggtg catagcggcg cagggtggcg tagctctcgc cgatgcgatg caggaaatcg
    40561 aagtcatcgg gttgcgcgag cttctgcgcc tcggtgacgc tctcggcaaa ggaatcccag
    40621 gacatgacgg cctcgatggc ggcaaacgca tcgcggcctg attgcttggc gtcgatcagc
    40681 gcctgaccga tgcgcccgta cagacgtacc ttggcgttga tggccttgcc tgacgcctgg
    40741 aactgctgct gatgcttatt cttggcagcg ttaaacagct tacccaggat gcggtcgtgc
    40801 aggtcgatga tttcgtcggt gacggtggcc atgccctcgg tggccagcgc cacgagagtg
    40861 gcgtagcgcc gttgcggctc gaatttggcc aggtcggcgg gtgtcatctg gccgccctcg
    40921 cgggcaatct tgagcaggcg gttctggtga accagccgct cgatgccggt aggcagatcg
    40981 agtgcctgcc atgccttgag gcgttcgatg tgttccagca tatgccgcga atttggcttg
    41041 gccggagact ggcgcaacca agccaaccag gtcgtcttgc cgttgtcccg gcgcttgagc
    41101 agatcgtcga ggcggcggcg atgcgcgtcc gccagtggtt cggccaaggc gtcgtagatg
    41161 cgccggttag cacgggtgat cgcctcggca ctcgcccgct cgacggcgtt gagggcgggc
    41221 agaatgaccg actgccgccg caggtgcccg atcaaggcgc tggccagcac gatgcctttg
    41281 tcggtttgca tcgccagctc ggtcagcatc tggacggcct gccggtaatg gctcatggtg
    41341 aagggccgga aaccgaacac ggtttgcagc tcgctcaggt gctcgcgccg ggtctgctcc
    41401 cgctggccgt actcgttcca gctttcgacg ccgaccttga gctggtcggc gaccagcttc
    41461 aacaagggcg ggaacggtag ttcatcgacg cccaggatga cgccgggaaa gcgcaggtaa
    41521 cagagctgca ccgcgaagcc cagccgattg gctggcccgc gccgctgtcg gatgatcgag
    41581 aggtcggtat cgttgaatgt gtaatgtcgg atcaggtcgt ccttggagtc cggcaacgcc
    41641 agcaggcttt cccgctcggc ggcggacagg atggaacgac gtggcatatt tactgatccg
    41701 ttctcaagta ttgatacagg gtttcgcgac tgattccgaa ttcacgagca agcttggtct
    41761 ttggctcgcc agcctcgaca cgttggcgca gttcggcaat acgctcagac gacagggatt
    41821 tcttcctgcc acggtaagcc ccgcgttgct tggcgagcgc aataccctcg cgctgacgct
    41881 cgcggatcag ggcgcgctcg aactcggcga acgcgcccat caccgagagc atcaggttcg
    41941 ccatcggaga gtcttcgcca gtaaaactga ggtgttcctt gacgaattcg atatgcacgc
    42001 cgcgttgtgt cagcgtttgc acgatccggc gcaaatcatc gagattgcgc gccaggcgat
    42061 ccatgctatg caccaccacg gtgtcgccgg tgcgggcgaa gcttatcagc gcttccagtt
    42121 gcggacgctt gacatccttg ccggatgcct tgtcgctaaa agcgcgatca accttgacgc
    42181 cttccagttg ccgttccggg ttctggtcga aggtgctgac cctgatatac ccaatgcgct
    42241 gtccagtcat ggaattccct gcaaaatgtc agggaagact ctatgacctt caacgagata
    42301 tgtcaataaa ttcaaaattc aatcctatcc tgacgcaatt tacacatggc atctgacatc
    42361 aggttagggt atgcctcaac ctgacggcgg cgaatcacaa gcgtccggtt tgacgctggt
    42421 ttcggtcgca gtgctggccc gcgcctggaa gcgctgatag cactccagcc cgcagaaatg
    42481 ctccacgtac tcggcccctt ccggcgtgaa ggcggcatcg agcgggattt ccttgcagca
    42541 cacgcagcaa ctggtgctcg gttcattggc gttcatggtg gtgttcctcc attggttgac
    42601 gaagccgacg aaggccgccg ccggcatcgg cctggcgaac aggaaaccct gcaccgtgtc
    42661 gcaacccgcc tgccgcaacc acgcaaggca gtcgggtgtt tccacaccct cggctaccac
    42721 ctccattgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact gcactatagc
    42781 agcggagggg ttggatccat caggcaacga cgggctgctg ccggccatca gcggacgcag
    42841 ggaggacttt ccgcaaccgg ccgttcgatg cggcaccgat ggccttcgcg caggggtagt
    42901 gaatccgcca ggattgactt gcgctgccct acctctcact agtgaggggc ggcagcgcat
    42961 caagcggtga gcgcactccg gcaccgccaa ctttcagcac atgcgtgtaa atcatcgtcg
    43021 tagagacgtc ggaatggccg agcagatcct gcacggttcg aatgtcgtaa ccgctgcgga
    43081 gcaaggccgt cgcgaacgag tggcggaggg tgtgcggtgt ggcgggcttc gtgatgcctg
    43141 cttgttctac ggcacgtttg aaggcgcgct gaaaggtctg gtcatacatg tgatggcgac
    43201 gcacgacacc gctccgtgga tcggtcgaat gcgtgtgctg cgcaaaaacc cagaaccacg
    43261 gccaggaatg cccggcgcgc ggatacttcc gctcaagggc gtcgggaagc gcaacgccgc
    43321 tgcggccctc ggcctggtcc ttcagccacc atgcccgtgc acgcgacagc tgctcgcgca
    43381 ggctgggtgc caagctctcg ggtaacatca aggcccgatc cttggagccc ttgccctccc
    43441 gcacgatgat cgtgccgtga tcgaaatcca gatccttgac ccgcagttgc aaaccctcac
    43501 tgatccgcat gcccgttcca tacagaagct gggcgaacaa acgatgctcg ccttccagaa
    43561 aaccgaggat gcgaaccact tcatccgggg tcagcaccac cggcaagcgc cgcgacggcc
    43621 gaggtcttcc gatctcctga agccagggca gatccgtgca cagcaccttg ccgtagaaga
    43681 acagcaaggc cgccaatgcc tgacgatgcg tggagaccga aaccttgcgc tcgttcgcca
    43741 gccaggacag aaatgcctcg acttcgctgc tgcccaaggt tgccgggtga cgcacaccgt
    43801 ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
    43861 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
    43921 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
    43981 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
    44041 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag
    44101 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
    44161 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
    44221 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
    44281 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
    44341 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
    44401 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
    44461 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
    44521 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
    44581 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
    44641 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
    44701 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
    44761 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
    44821 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
    44881 aatgtctaac aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta
    44941 gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat
    45001 ttttaagcgt gcataataag ccctacacaa attgggagat atatcatgat gactgatccc
    45061 cgcaaaaacg gcgatttgca cgaacccgcg acggcacccg cgacgccctg gtccaaaagc
    45121 gagctggtcc ggcaattgcg cgacctcggc gtgcgctcag gcgatatggt gatgccgcat
    45181 gtgtcgttgc gcgccgtcgg gccgctggcg gacggaccgc agacacttgt cgatgcgctg
    45241 atcgaggccg tcggccccac cgggaatatt ctcgccttcg tctcgtggcg cgattcgccc
    45301 tatgaacaga cgctgggtca tgatgcgccg cccgccgcca tcgcccaaag ctggcctgcg
    45361 ttcgaccccg accatgcgcc cacctacccc ggctttggcg cgatcaacga atttatccga
    45421 acctatccgg ggtgtcggcg cagcgcccat cccgacgcat cgatggcggc gatcgggccc
    45481 gatgcggcgt ggctggtggc gccgcacgag atgggcgccg cttatggccc ccgctcgccg
    45541 atcgcgcgtt ttctcgccca cgcaggaaaa atcctgtcga tcggcgccgg gcccgatgca
    45601 gtcaccgcgc tccattatgc cgaagcggtg gcgcggatcg agggcaagcg ccgcgtcact
    45661 tattcgatgc ccttactgcg cgaaggcaag cgcgtctggg tcaccacgtc cgactgggat
    45721 tcgaacggca tcctcgacga atatgccgcg cccgacggcc ccgacgcggt cgaacggatc
    45781 gcccgcgact atctcgcccg caccagggtt gcgcaaggcc cggtcggcgg cgcgcaatcc
    45841 cggctgatcg acgcggccga tatcgtttcc ttcggcatcg aatggctcga ggcgcgccac
    45901 gccgcgccag cggcggcagc gctgaagccg aaacaacgcc gcgactgagt cgtcgattat
    45961 tccggacata tattcctccc tctcgcccag ccgcatagca tcaggcccac aagcccgccg
    46021 cctcttcaac aaggatgtcg ggacgttctt cagtaaagaa cagcttggca ccctcgacaa
    46081 ggatgattgc ccgaggcgat caggcggcga atttttgcgc gcgcgcacgg tcgcggcggc
    46141 gcgcgataaa ggcgatgggt agccccacga ggaagatatg cgcggcgatt aacatcaagc
    46201 cgctgtacga ccgcgtcgtc atcaagcgca ccgaggaaga gaaggtctcc gccggtggta
    46261 tcgtgatccc ggattcggcc accgagaagc cgatcaaggg ccaggtcgtc gccgtgggcg
    46321 agggcaaggt gttcgacaac ggcaacgtgc gcgcgccgaa ggtcaaggtc ggcgaccagg
    46381 tgctgttcgg caagtacgcc ggcaccgagg tcaagctcga cggcaccgag tacctggtgg
    46441 tgaaggaaga cgacatcttc gccgtgctgg gctgagcccg ccgcttcgcc gcatcccaac
    46501 ccgcaatcac agaactgagg taattccgca atggctgcca aggatattcg tttcggtgaa
    46561 gacgcccgcg cccgcatggt ccgcggcgtg aacgtgctcg ccaacgccgt caaggcgacc
    46621 ctgggcccga agggccgcaa cgtcgtgctc gagaagagct tcggcgcccc gacgatcacc
    46681 aaggacggcg tgtccgtcgc caaggagatc gaactggccg acaagttcga gaacatgggc
    46741 gcgcagatgg tcaaggaagt cgcttccaag acctccgaca acgccggcga cggcaccacc
    46801 accgccaccg tgctggccca ggccctgatc cgcgagggca tgaaggccgt ggccgccggc
    46861 atgaacccga tggacctcaa gcgcggcatc gacaaggccg tcacctcggc cgtcgaggag
    46921 ctgaagaaga tctccaagcc ctgctcgacc agcaaggaga tcgcccaggt cggttcgatc
    46981 tcggccaact ccgacaccga catcggcgag ctgatcgcca aggccatgga caaggtcggc
    47041 aaggaaggcg tgatcaccgt cgaggagggc tcgggcctgg agaacgagct cgacgtcgtc
    47101 gagggcatgc agttcgaccg cggctacctg tcgccgtact tcatcaacaa cccgcagtcg
    47161 atgcaggccg agctggagga tccgttcatc ctgctgcacg acaagaagat ctcgaacgtc
    47221 cgcgacctgc tgccgatcct cgagggcgtg gccaaggccg gcaagccgct gctgatcgtc
    47281 gccgaggacg tcgagggcga ggcgctggcc acgctggtgg tcaacaccat ccgcggcatc
    47341 gtgaaggtct gcgcggtcaa ggccccgggc ttcggcgacc gccgcaaggc gatgctggag
    47401 gacatggcca tcctcaccgg tggcaccgtg atctccgagg aagtcggcct ctcgctcgag
    47461 aaggcgacca tcaacgacct cggccgcgcg aagaaggtgc aggtctcgaa ggagaacacc
    47521 accatcatcg acggcgccgg cgacaccgcg gacatcgaag cccgcatcaa gcagatcaag
    47581 gcgcagatcg aggagaccac ctcggactac gaccgcgaga agctgcagga gcgcgtggcc
    47641 aagctggccg ggggcgttgc ggtgatcaag gtcggcgccg ccaccgaagt cgagatgaag
    47701 gaaaagaagg cgcgcgtcga agacgccctg catgccaccc gtgcggcggt cgaggaaggc
    47761 atcgtcccgg gcggcggcgt cgccctgatc cgtgccaagg ccgcgatcgc cgagctgaag
    47821 ggcgccaacg aggaccagaa ccacggcatc gcgatcgccc tgcgcgcgat ggaagccccg
    47881 ctgcgcgaga tcgtcaccaa cgccggcgac gagccgagcg tggtgctgaa ccgcgtcgcc
    47941 gaaggcaccg gcgcgttcgg ctacaacgcc gccaacggcg agttcggcga catgatcgag
    48001 ttcggcatcc tggacccgac caaggtcacg cgctccgcgc tgcagaacgc ggcgtccatc
    48061 gccggcctga tgatcaccac cgaagcgatg gtggccgaag cgccgaagaa ggaagagccg
    48121 gccgctccgg gcggcggcat gggcggcatg ggcggcatgg atttctaagc cccgcgatcc
    48181 atcaagcaag accacaaagc ccggcctcgt gccgggcttt gtgcgttctg gcgtccgagg
    48241 cgggagactt cctacccgcc ccgcggcaat gtctgacgcg aagatcagaa aacgccgata
    48301 tgaacgcgtg ctcgcgggcg caaccctgag cagccgtccc tgcaacggag cgctgcgtgc
    48361 cgcgcctgac cgcaccccgg cggcaggccg aggtgtgcgc gccactgccg gccgcccacg
    48421 ccgctgcgcg ttacgcgcgc cacctgcccg agcgcacgct gctgtacgcg ctagtgcagg
    48481 cgcactaccc ggacttcatc gcgcgtcttg aggccgaaga ccgcccgctg cccgagtatg
    48541 tgcgcgagga gttcgagacc tacctgcgtt gcggcgtgct cgagcacggt ttcctgcgcg
    48601 tggtgtgcga gcagtgccgg gccgagaggc tggtggcctt ctcctgcaag aagcgcggct
    48661 tctgccccag ctgcggggcg cggcgcatgg ccgagagcgc gcggcacctg gtggaggagg
    48721 tgtttggccc gcggccggtg cggcaatggg tgctgagttt tccgtacccg ctgcgcttcc
    48781 tgttcgccag caagccggag gccatcggcc cggtgctggg catcgtgcat cgtgtgatcg
    48841 ccggctggtt ggccgatcaa gccggcgtcg accacgccag cgcgcaatgc ggcgcggtga
    48901 ccctgatcca gcgcttcggc agcgcgctga acctgaatgt tcacttccac atgctgtggc
    48961 tcgacggcgt gtacgaggac accaccgagc gtccgcagcg caagccgcgc ctgcaccgca
    49021 cccgtgcgcc cacatcggcg caactgacgg aactggccgg caccatcgcg catcgcgtgt
    49081 gccggcacct gtcgcgccgc ggctggctcg aaggcgaaga cgaatccgtg ttcctgtccg
    49141 acagcgcggg tagcgacgac ggcatggatg ggctgcggat gagttcgatg acctaccgca
    49201 tcgccaccgg tcgcgacgct ggccgcaagg tcgtcacgct gcaaacattg cccggtgacg
    49261 caggctcgct ggagggcgac gccggcaagg tcggcggctt ctcgctgcat gccggcgtgg
    49321 ccgcggaagc acacgaaagc cacaagctcg aaaagctgtg ccgctacatc acgcgcccgg
    49381 cgatcagcga gcagcggctg tcgatctcac cgcagggtag ggtgcggtat cagctcaaga
    49441 cgccgtggcg aaatgggacc acgcatgtcg aatgggatgc ggtggacttc atcgccaagc
    49501 tggcggcact ggtcccgcca cctcgcgcgc atctcacccg cttccacggc gtattcgccc
    49561 cgaatgcaaa cctgcgtgcg cagctgacgc cctcggggcg cggcaggcgg cctgcgggcg
    49621 atgcggtgcc ggttgacgca agcgcccacg acgagccgcg cagccccgag cagaagcgcc
    49681 gtgcgatgag ctgggcgcaa cggctcaagc gggtcttttc catcgacgtc accacctgtg
    49741 cccactgcgg cggcgcggtg cggatcgtcg ccagcatcga agaccccaag gccattcgcg
    49801 ccatcctcgc ccacttcgag aaacacggcg cgctggagca agcgcactac cggcccgcag
    49861 cgcgcgcgcc gccgccggcc gcgtgacgag gtgccggcca cacagccggc agccaagcca
    49921 gagtccgatc cgatgcggcc acgaccccgc agggctgcgc tcggccctgt gccgggtttc
    49981 ggtgagaaat ggctacgcac tgagccgctg cgtggccccg cgatgtcgaa aacccacgca
    50041 tgaacccccg atctgtgccc gatctgtgcc caaagcggcg cttgcgcggc cgcttccgag
    50101 ggtgtcagaa attttgtgtg agaggcatac catgatgctg ccaaggagca tgtatgccac
    50161 gcaagaagaa gacgccggtg gtcacgccgg ccattgatct gccgaaggaa tttctcgaga
    50221 agctgatccc gggtccgatg gacgcggccg gggtcgaagc cgtgttccag cagctgaaga
    50281 aggccgtgat cgagcgggcg ctgggcgcgg agctcggcct gcacctggcc gacgccgagg
    50341 gcgggagcgg caaccaccgc aacggccgca gcggcaagac ggtgctgacc gacgagggtc
    50401 cgctgcgcat cgacgtgccg cgcgaccgga cgggtacgtt cgagccgcag ctgatcccca
    50461 agcacgagcg tcgcttcgcc gggttcgacg atcggatcgt ctcgatgtac gcccgcggca
    50521 tgacggtgcg tgagatccag gggcacctgg ccgagatgta cagcgtcgag gtgtcgccgg
    50581 agttcatcag caaggtcacc gacgaggtga tggccgaggt caccgcatgg caggcgcggc
    50641 cgctggaagc gatgtatccg gtggtgttct tcgacgcgct gcgggtgaag atccgcgagg
    50701 acggcgtggt gcgcaacaag gcggtgtatc tggcgctggg cgtgctggcc gacggcaccc
    50761 gcgacatcct cgggctgtgg atcgagaaca ccgagggcgc gaagttctgg atgaaggtgt
    50821 tcaacgacct caagacgcgc ggcgtcaacg acatcctgat cggcgtggct gacggcctga
    50881 agggcttccc ggaggcgctg gaagcggtgt tcccggcgac cacgctgcag acctgcatcg
    50941 tgcacctgat ccgcaacagc ctggactatg cgggctggaa ggaccgcaag gcgctggcgg
    51001 cggcgatcaa gccgatctac acggccccga ccgccgaggc cgcagaggcc gagctggatg
    51061 cgttcgaggc cggcgactgg ggcgagcgct tcccgacggt ggtcgccgcc tggcgccgtg
    51121 cctggacgcg cgtggtgccg ttcttcgcgt tcccgccgga cgtgcgccgg gtgatctaca
    51181 cgaccaacgc aatcgagagc gtgcatgcgc gcctgcgcaa gatcatcaag acccgcggcc
    51241 acttccccag cgacgaggcc gccaccaagc tgatctggct ggcgctgcgg aacatcaccg
    51301 ccgagtggag ccgcgcgtct cagcactgga aggcggcgat gaaccagttc gcgataatct
    51361 acgaagaccg attcacgctg gctcgctgaa agagacttcc ggactaaacc gcctcacaca
    51421 caaaaaatcg gacacgcccc cgcttcctac ctgccagact cgcaaaaaag ggcggttgaa
    51481 cttcctatac ccacaaagtt agatgcacta agcacataat tgctcacagc caaactatca
    51541 ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca aattgggaga
    51601 tatatcatga aaggctggct ttttcttgtt atcgcaatag ttggcgaagt aatcgcaaca
    51661 tccgcattaa aatctagcga gggctttact aagcttgccc cttccgccgt tgtcataatc
    51721 ggttatggca tcgcatttta ttttctttct ctggttctga aatccatccc tgtcggtgtt
    51781 gcttatgcag tctggtcggg actcggcgtc gtcataatta cagccattgc ctggttgctt
    51841 catgggcaaa agcttgatgc gtggggcttt gtaggtatgg ggctcataat tgctgccttt
    51901 ttgctcgccc gatccccatc gtggaagtcg ctgcggaggc cgacgccatg gtgacggtgt
    51961 tcggcattct gaatctcacc gaggactcct tcttcgatga gagccggcgg ctagaccccg
    52021 ccggcgctgt caccgcggcg atcgaaatgc tgcgagtcgg atcagacgtc gtggatgtcg
    52081 gaccggccgc cagccatccg gacgcgaggc ctgtatcgcc ggccgatgag atcagacgta
    52141 ttgcgccgct cttagacgcc ctgtccgatc agatgcaccg tgtttcaatc gacagcttcc
    52201 aaccggaaac ccagcgctat gcgctcaagc gcggcgtggg ctacctgaac gatatccaag
    52261 gatttcctga ccctgcgctc tatcccgata ttgctgaggc ggactgcagg ctggtggtta
    52321 tgcactcagc gcagcgggat ggcatcgcca cccgcaccgg tcaccttcga cccgaagacg
    52381 cgctcgacga gattgtgcgg ttcttcgagg cgcgggtttc cgccttgcga cggagcgggg
    52441 tcgctgccga ccggctcatc ctcgatccgg ggatgggatt tttcttgagc cccgcaccgg
    52501 aaacatcgct gcacgtgctg tcgaaccttc aaaagctgaa gtcggcgttg gggcttccgc
    52561 tattggtctc ggtgtcgcgg aaatccttct tgggcgccac cgttggcctt cctgtaaagg
    52621 atctgggtcc agcgagcctt gcggcggaac ttcacgcgat cggcaatggc gctgactacg
    52681 tccgcaccca cgcgcctgga gatctgcgaa gcgcaatcac cttctcggaa accctcgcga
    52741 aatttcgcag tcgcgacgcc agagaccgag ggttagatca tgcctagcat tcaccttccg
    52801 gccgcccgct agcggaccct ggtcaggttc cgcgaaggtg ggcgcagaca tgctgggctc
    52861 gtcaggatca aactgcacta tgaggcggcg gttcataccg cgccagggga gcgaatggac
    52921 agcgaggagc ctccgaacgt tcgggtcgcc tgctcgggtg atatcgacga ggttgtgcgg
    52981 ctgatgcacg acgctgcggc gtggatgtcc gccaagggaa cgcccgcctg ggacgtcgcg
    53041 cggatcgacc ggacattcgc ggagaccttc gtcctgagat ccgagctcct agtcgcgagt
    53101 tgcagcgacg gcatcgtcgg ctgttgcacc ttgtcggccg aggatcccga gttctggccc
    53161 gacgccctca agggggaggc cgcatatctg cacaagctcg cggtgcgacg gacacatgcg
    53221 ggccggggtg tcagctccgc gctgatcgag gcttgccgcc atgccgcgcg aacgcagggg
    53281 tgcgccaagc tgcggctcga ctgccacccg aacctgcgtg gcctatacga gcggctcgga
    53341 ttcacccacg tcgacacttt caatcccggc tgggatccaa ccttcatcgc agaacgccta
    53401 gaactcgaaa tctaacgtcc gttcgggcat cgaggtccat gtcggggtgg gacgggcccg
    53461 tggcttcaag atcacttgca gtccgaccgc gatgtcttgg ttgcgcgaga ggttgtcgat
    53521 atctgttgat ttgcacccaa atttgacccg ggatttgcat tgaattttga cccacccctt
    53581 gttgtcagaa ttatgtctcg attttcagtt tgcgggtctg tttttcctcc tgcttattct
    53641 gagttgaact gtgtttgaag cggtaacttt cattgccggt ttccaggatg tggcagtggt
    53701 gggttagtcg gtccaacaac gctgttgtca tcttttcatc gccaaacact cggctccatt
    53761 ccgagaagct caagttggtg gtcagtatca cgctggtttt ttcgtacagc tttgagagca
    53821 ggtgaaacag cagtgcccca ccggtttggc taaaaggcaa atatcccagc tcatccagaa
    53881 tcaccaaatc ggcatacaac agacggtttg cgatttgtcc ctgacgccca gatgatttct
    53941 cttgctccag tgcattgacc aaatccacgg tggagaagaa acgcacccgt cggttcaagt
    54001 gcatcactgc ttgtgtacca atggctgtgg ccaggtgagt cttgcctgtg cctggcccac
    54061 caatcagcac cacgttctgg gcttgttcca tgaagtcgca ccggtgcaat tgtttgaccg
    54121 tggcctcatt aaccaggctt tgactgaagt caaagcccac caagtcccga tacacgggga
    54181 acttggccac ccgcaattga tagttcaccg aacgtacttc acgctctgcc acttcagctt
    54241 taatcaagct gtccagcatg ggcaaggctt gattaaatgc tggtgaattc tgattgccca
    54301 actcctcaat ggcgtgtgcc atgccaaaga gtttcaagga tttgaggatt ctcacatggc
    54361 cttcatgctg catcatgggc tctccttaaa ctgtcatagc ggttcacgtt ggcctgtggt
    54421 tccaatgtca gccttaaccc cttgggaatt ggaatcggtt tgggtggagg ttcttcggtc
    54481 aaacgtccca acagattaag cacatgctcc ttcgatggct tgccacactc caatgccaat
    54541 tccacagcac tgagtaccgc accttcatcg tggtgcaata caagggccag aatttccacc
    54601 atgtcacggt caccgccggg gcgttgcagc aagatggatt gaagcttctt gaacgcgggt
    54661 ggcaattcag caaatggcgc accattgcgc aacgccccag gtttcttctg aagcacagac
    54721 aagtaatggt gccagtcgta ttgtgtgtgg ccacgccgag cgtggccact gccaaacaat
    54781 cttggatgct cggcaatgtg ttggccttcg gcagccatca ccagcttgtc tgcataaatc
    54841 cgaaggctga tggccctgtt ggcgtaactg gcaggaacgc tgtagcgatt gccctcgtgg
    54901 tgaacaaggc aggttgaagt gactcgcttg gtttgctcca cgaatgcatc aaaggcattg
    54961 ggtagcgcca tcaactcgcc ttgttcatcg gcaaaggcct cttgcacggt ttggtccaat
    55021 tcggggtggc gcagctcaga ccacagcgct ttgcagcgat gctcaagcca cacattcaaa
    55081 tcagcaaggc tttgaaagtc tggtgcccct tgccacaggc gttggcggga atcctgcacg
    55141 ttcttctcaa tctggccttt ctcccaaccc gatgctggat tacagaactg cgcatcaaac
    55201 aggtagtggc tgaccatggc agtgaaccgc tgattgaccc tgcgctcttt gccacgcccc
    55261 accgaatcca cagcggtctt catgttgtcg tagatgccgc gcttgggaat gccaccgaag
    55321 atttgaaagg catgccagtg ggcatcaaac agcatttcat gtttttgctg gtagtaagcc
    55381 cgaagcacaa aggcccggct gtgggccaac ttaaactggg caatctgaag tttgacctgt
    55441 ttgcccgcta tgcgggcaaa gtcctcactc caatcgaatt ggaaggcttc gccacaagca
    55501 aagcgcaagg ggatgaaaca acccttgccc gaggtttgcg ccttgaactg ttcggaatct
    55561 ttccactgtc gggcaaaggc acacactcgg tcataagacc cggtaaagcc caaagcgacc
    55621 aaatcccggt acatgctgcg caggtttctg cgcagcttct ttgtcttttt gtgctcggtg
    55681 gagagccact gccttaactt gggctcaaaa ggacttaact tgccaacgct gtctcgcgct
    55741 gggtactgcg gttcaaccac cttgctttgc aaatacttgc gaacggtgtt cctggacagg
    55801 ccgcttcgtc gggctatttc ccgaatcgac gcaccatcgc gaaaatgcca gcgtcgaatt
    55861 gcgctcaata tcgccacgtt tatcactcct tgatttctcc cgccatatcc agacgggaaa
    55921 cagtgtcata cgtgggtcaa atttcgacgc aaatctttac cctaagtggg tcaattttag
    55981 atgcaactca acaggccatg ctgagtgtgc gatggttgat cgcttcctcg ccgctctcca
    56041 cggcgacgat ggccgccgcc atcagcaagt gcgccagttc ccctatggtg ccctcgctgc
    56101 gtgtgagcag gtagcgagcc atgtccagcg tggcaattgg ggaaggccgg cgcagcggga
    56161 gcgaagcggc gaagctggcc agcagtgagc agcaatcgtc gttggcctcc cataccggca
    56221 gcatcatcgg ctcgaagcga ttttccaact ggtcatcgga gcggatggct aggtaggcgt
    56281 cgcgcgtgcc taccccaacc aacgggatgc gcagttcgtt gccgaggaag cgcagcaggt
    56341 tgaggaattc ccggcggttg acgctgttgc cggccagcac gttgtgcagc tcgtcgatca
    56401 ccagcatgcg cacgccgacc ttgcgcagca gtgccagagc cagttgctcc atttccggca
    56461 accgtgggcg tgggcgcagc ggcgcgccca tcgcggcgag cagcgcgacg tagaagcgga
    56521 tcacggacgg ctcggacggc atctgcacga ccaacaccgg gatgtgctcc tggtcggcgt
    56581 cggagctggc cgggtgggtg cggcggaact tctcgacgat catcgacttg ccattgttgg
    56641 tcgggccaac cagcagcagg ttgggcatgc gttgcttgtt tggccacgca taaagggctt
    56701 ccagccggtt cagcgcctcg actgcgcgcg gatagccgat ccagcggtcg gcgcgaaggc
    56761 gctggatgcg ctcgtccgcc ggaagacggg ccaagccctg ggccgccggc agcaggtggg
    56821 acaggtcgat gatgggatat tcgtccacgg ctaccactcc tcaatctggt cgaacggttt
    56881 ggcgggtggc aagttgtctg cctgcgggtc ggcaatatcc gtatccggcg gaacgggctt
    56941 gtccggccga gctgatgtct tgaggtgctg gcggcgatcc gcgtcacgcc gcgccttgcg
    57001 tgtggccttc tgcgcgctgg tcacaatctc acgcatctgg ccgatcatgc ggaacagcgc
    57061 cgactcatcc acctgttcgc gcccttgctg ccgcagtttc gccagcgcct gccgttgttc
    57121 ccagagggtg acagccggat gcgacaaggt acggtaggga atttccaggt aatgctgtcc
    57181 ctccggttcc aggacccaga tacggctgat gtcgcgcgga tcgcgccgga tcagaaagga
    57241 cggccagcgt tcacgccgcg caatccacgg cttgagcgca tcggcgtagt agtggatgtg
    57301 gtcgatgaca aagccggtgc gggtcagcgt gcgccggagg atcggcagaa aatcgaccag
    57361 gaacgaagta gcgcgtgtga cgacggccgg tacgccgaca cgcgccacgg cctcggccca
    57421 gcgcgcggcc ggcggttgga gcaggccgtt gtgcaccgaa ccgtggtagg tgccgaccgc
    57481 caatgtgagc cagcgctcta gctcgcgcag cgtcagggcg gccttgtttt cggaatcgta
    57541 gtcgccgcgc tggtcagggt tggagaaggt cgttcccggc agttcgtcgt gaatcatctg
    57601 catcgccgtg ccgatgatcc gttccacgat gccgccatag tgcggctgtc ccagcgggcg
    57661 atagtccagc cggatgccat gctgctcgca accccggcgc agggcctcgc tcttgaactc
    57721 ggccgcgttg tctaggtaga gcagcaaggg cttgccgctc atctgccaat ccatttccac
    57781 gttcagtcct tccagccaag ggcgcttgtc gcaggcgaca tgcacgaggc acaggccaac
    57841 cgaaacggca gacggcgctt ccagcgtgac gaccatgccg agcacgcagc gggtgaacac
    57901 gtcgatggcg agggtcaggt acgggcggcc aataggttgc cggtcgcggt catcgaccac
    57961 gatcaggtcg atgaccgtat ggtctatctg cacctgctcc agcggcgcgg tcacggcagg
    58021 aggctcgccg cccacacctt gtaggtcacg agcggcatcc tggccttccc gccggcggat
    58081 gaccttgcgc gggtcaaggc tagcgatccg taaggccacg gtattgcgcg ccggcactcg
    58141 cagtttttga gccttgcaca cctgagtgac ttcgcggtga aaggccgcta ggctgcgctt
    58201 ctgcttggtc aggaaccgct tttgcagtag ctcgtggatg acgcgctcga ccggttccgg
    58261 caagcgcccc ttacctttac ctccaccgga ctggccgggc accagatccg tcacgaggcc
    58321 gctgccttgc cgggcacgcc ggatcagaac gtatacctgg cgccgagaca agcccagcgc
    58381 ctgagccgcc atatcggccg cttcgtgccc gaccgtctcc gactgcgcca acggactgat
    58441 g
//
LOCUS       DAMZET010000010        52698 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.010, whole genome shotgun sequence.
ACCESSION   DAMZET010000010 DAMZET010000000
VERSION     DAMZET010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.010"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..888
                     /locus_tag="POB34_000754"
     CDS             <1..888
                     /locus_tag="POB34_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CS1-pili formation C-terminal domain-containing
                     protein"
                     /protein_id="HDI5917749.1"
                     /translation="SWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGN
                     VYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSE
                     QDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIIN
                     GAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGF
                     TLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQ
                     LKQPKLRGNVQTAQREVAP"
     gene            885..1973
                     /locus_tag="POB34_000755"
     CDS             885..1973
                     /locus_tag="POB34_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HDI5917750.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(2061..3359)
                     /gene="exuT"
                     /locus_tag="POB34_000756"
     CDS             complement(2061..3359)
                     /gene="exuT"
                     /locus_tag="POB34_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HDI5917751.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            3842..5254
                     /gene="uxaC"
                     /locus_tag="POB34_000757"
     CDS             3842..5254
                     /gene="uxaC"
                     /locus_tag="POB34_000757"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDI5917752.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGEPLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            5269..6756
                     /gene="uxaA"
                     /locus_tag="POB34_000758"
     CDS             5269..6756
                     /gene="uxaA"
                     /locus_tag="POB34_000758"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDI5917753.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            6839..7390
                     /locus_tag="POB34_000759"
     CDS             6839..7390
                     /locus_tag="POB34_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HDI5917754.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEVSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(7395..8639)
                     /gene="sstT"
                     /locus_tag="POB34_000760"
     CDS             complement(7395..8639)
                     /gene="sstT"
                     /locus_tag="POB34_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDI5917755.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(9038..10003)
                     /gene="alx"
                     /locus_tag="POB34_000761"
     CDS             complement(9038..10003)
                     /gene="alx"
                     /locus_tag="POB34_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HDI5917756.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(10286..11272)
                     /locus_tag="POB34_000762"
     CDS             complement(10286..11272)
                     /locus_tag="POB34_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDI5917757.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(11351..12034)
                     /locus_tag="POB34_000763"
     CDS             complement(11351..12034)
                     /locus_tag="POB34_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HDI5917758.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(12111..12614)
                     /locus_tag="POB34_000764"
     CDS             complement(12111..12614)
                     /locus_tag="POB34_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDI5917759.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            12699..13835
                     /gene="rlmG"
                     /locus_tag="POB34_000765"
     CDS             12699..13835
                     /gene="rlmG"
                     /locus_tag="POB34_000765"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HDI5917760.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKTVKLGRRR"
     gene            14026..14109
                     /locus_tag="POB34_000766"
                     /pseudo
     CDS             14026..14109
                     /locus_tag="POB34_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            14106..14522
                     /gene="higA"
                     /locus_tag="POB34_000767"
     CDS             14106..14522
                     /gene="higA"
                     /locus_tag="POB34_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HDI5917761.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(14567..16585)
                     /gene="fadH"
                     /locus_tag="POB34_000768"
     CDS             complement(14567..16585)
                     /gene="fadH"
                     /locus_tag="POB34_000768"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HDI5917762.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSAFQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPSVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(16911..19262)
                     /gene="ygjK"
                     /locus_tag="POB34_000769"
     CDS             complement(16911..19262)
                     /gene="ygjK"
                     /locus_tag="POB34_000769"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDI5917763.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDHLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTSQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDRVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWVAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWMVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(19279..20349)
                     /gene="ygjJ"
                     /locus_tag="POB34_000770"
     CDS             complement(19279..20349)
                     /gene="ygjJ"
                     /locus_tag="POB34_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HDI5917764.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            complement(20483..21916)
                     /locus_tag="POB34_000771"
     CDS             complement(20483..21916)
                     /locus_tag="POB34_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI5917765.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(21979..22428)
                     /locus_tag="POB34_000772"
     CDS             complement(21979..22428)
                     /locus_tag="POB34_000772"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HDI5917766.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(22425..25517)
                     /gene="ebgA"
                     /locus_tag="POB34_000773"
     CDS             complement(22425..25517)
                     /gene="ebgA"
                     /locus_tag="POB34_000773"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HDI5917767.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLSASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIIRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(25701..26684)
                     /gene="ebgR"
                     /locus_tag="POB34_000774"
     CDS             complement(25701..26684)
                     /gene="ebgR"
                     /locus_tag="POB34_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HDI5917768.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQLLAQEDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            26903..27235
                     /locus_tag="POB34_000775"
     CDS             26903..27235
                     /locus_tag="POB34_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HDI5917769.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            complement(27277..28656)
                     /gene="ygjG"
                     /locus_tag="POB34_000776"
     CDS             complement(27277..28656)
                     /gene="ygjG"
                     /locus_tag="POB34_000776"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HDI5917770.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            29074..30594
                     /gene="aer"
                     /locus_tag="POB34_000777"
     CDS             29074..30594
                     /gene="aer"
                     /locus_tag="POB34_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HDI5917771.1"
                     /translation="MSSHPYVTQQNTPLAGDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVMLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(30748..31371)
                     /gene="nfeR"
                     /locus_tag="POB34_000778"
                     /gene_synonym="yqjI"
     CDS             complement(30748..31371)
                     /gene="nfeR"
                     /locus_tag="POB34_000778"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HDI5917772.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            31659..32423
                     /gene="nfeF"
                     /locus_tag="POB34_000779"
     CDS             31659..32423
                     /gene="nfeF"
                     /locus_tag="POB34_000779"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HDI5917773.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELVID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLEM
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            complement(32477..32552)
                     /locus_tag="POB34_000780"
     tRNA            complement(32477..32552)
                     /locus_tag="POB34_000780"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32517..32519),aa:Ile,seq:cat)
     gene            32677..33183
                     /gene="mug"
                     /locus_tag="POB34_000781"
     CDS             32677..33183
                     /gene="mug"
                     /locus_tag="POB34_000781"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HDI5917774.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            complement(33262..35103)
                     /gene="rpoD"
                     /locus_tag="POB34_000782"
     CDS             complement(33262..35103)
                     /gene="rpoD"
                     /locus_tag="POB34_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDI5917775.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHATAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(35298..37043)
                     /gene="dnaG"
                     /locus_tag="POB34_000783"
     CDS             complement(35298..37043)
                     /gene="dnaG"
                     /locus_tag="POB34_000783"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDI5917776.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            complement(37154..37369)
                     /gene="rpsU"
                     /locus_tag="POB34_000784"
     CDS             complement(37154..37369)
                     /gene="rpsU"
                     /locus_tag="POB34_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDI5917777.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            37607..38620
                     /gene="tsaD"
                     /locus_tag="POB34_000785"
     CDS             37607..38620
                     /gene="tsaD"
                     /locus_tag="POB34_000785"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDI5917778.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            complement(38663..40126)
                     /gene="ttdT"
                     /locus_tag="POB34_000786"
     CDS             complement(38663..40126)
                     /gene="ttdT"
                     /locus_tag="POB34_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HDI5917779.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(40174..40779)
                     /gene="ttdB"
                     /locus_tag="POB34_000787"
     CDS             complement(40174..40779)
                     /gene="ttdB"
                     /locus_tag="POB34_000787"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HDI5917780.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            complement(40776..41687)
                     /gene="ttdA"
                     /locus_tag="POB34_000788"
     CDS             complement(40776..41687)
                     /gene="ttdA"
                     /locus_tag="POB34_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HDI5917781.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            41894..42826
                     /gene="ttdR"
                     /locus_tag="POB34_000789"
                     /gene_synonym="dan"
     CDS             41894..42826
                     /gene="ttdR"
                     /locus_tag="POB34_000789"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HDI5917782.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            complement(42839..43456)
                     /gene="plsY"
                     /locus_tag="POB34_000790"
     CDS             complement(42839..43456)
                     /gene="plsY"
                     /locus_tag="POB34_000790"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDI5917783.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            43561..43929
                     /gene="folB"
                     /locus_tag="POB34_000791"
     CDS             43561..43929
                     /gene="folB"
                     /locus_tag="POB34_000791"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HDI5917784.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            44019..44840
                     /gene="bacA"
                     /locus_tag="POB34_000792"
     CDS             44019..44840
                     /gene="bacA"
                     /locus_tag="POB34_000792"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDI5917785.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTSGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(45021..46259)
                     /gene="cca"
                     /locus_tag="POB34_000793"
     CDS             complement(45021..46259)
                     /gene="cca"
                     /locus_tag="POB34_000793"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HDI5917786.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLALM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(46323..46943)
                     /locus_tag="POB34_000794"
     CDS             complement(46323..46943)
                     /locus_tag="POB34_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDI5917787.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            47185..48486
                     /gene="ygiF"
                     /locus_tag="POB34_000795"
     CDS             47185..48486
                     /gene="ygiF"
                     /locus_tag="POB34_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HDI5917788.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSQIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLHHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            48509..51349
                     /gene="glnE"
                     /locus_tag="POB34_000796"
     CDS             48509..51349
                     /gene="glnE"
                     /locus_tag="POB34_000796"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HDI5917789.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SISAHPEWLTELESQPPQADEWQHYVAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAELHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            51397..>52698
                     /gene="hldE"
                     /locus_tag="POB34_000797"
     CDS             51397..>52698
                     /gene="hldE"
                     /locus_tag="POB34_000797"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HDI5917790.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGD"
BASE COUNT        12459 a        13588 c        14266 g        12385 t
ORIGIN      
        1 tcatggcagt tatcgcatac cttttataac gattcattca gccataaaga acttggcgta
       61 accgtcggag gcctgaacac cgataccata aacagcgcgg ttaacgggcg ttgggatggg
      121 caatacggaa atgtctacgc taccgtatct gatagttatg accgtaagaa tcatgatcat
      181 ctttcggcct ttacgggcac ttatagctct acgctggctg tcagtcgcta tggcgttaat
      241 ttgggagcca gtggtacaga cgatttgctg ggtgcggtac tggtggatgt gaaaggcttc
      301 tctgaacagg atgaagagag tcaggatctg caactcgaag cgcgggtggc aggcagccga
      361 actttgcagc ttggtcaaag tgacagtgtg ttgttccctt atcctggatt tcagtctggt
      421 tttgttgagg ttaacgacag tagccagggc aatcagcagg ggacaacaaa catcattaac
      481 ggtgcaggga atcgtgaatt aatgttgttg cctggcaagc tgcgctatcg cgaagtgtct
      541 gccagcttta actacaacta tatcggtcgt ttattactgc cggcagcggt gaaaaaattc
      601 ccgattgtgg ggctgaacag cgccatgtta ctggtagctg aagatggcgg atttacactt
      661 gaaattaacg gcagcgaaaa agagctctat ctgctttccg ggcagcaatt ccttaagtgt
      721 ccactaagtg ttgtaaagaa acgcgccagc attcgttaca gcggagatgt tacttgtagt
      781 gtggtgactt attcacaatt accggagtcg attcaggttc aggcacagtt gaaacagcct
      841 aaattacgtg gaaacgttca gacggcgcaa agggaggttg caccatgaga aatcgattga
      901 ttgcggcgat attgggcttg tttggcacgc tcactggcgt tcaggcagct cctgacgtga
      961 ccagtgaaat tacgtatgat ttggcatctg gtagagcgga ttattacttc tggaaggatg
     1021 aagcgtcggc aggaaataat ggatatatgt ggtatgaatg ttcgtatcct gacctccaac
     1081 aaacctgtac agctaatgga aatatatcga cagtacaaat ctatttaact gaacaacgca
     1141 gtgggatgcg ttggccggta aaactcaaag gatttaaaac agccattgta agtagcgatg
     1201 aagcgccgcc aggatgcaag gggggcaaag ggcttcagac gaatcttaag gattctaata
     1261 gatcttcatg tacagaagat ggtcaacatt attatatata cgatacaaag tttcttacgc
     1321 tctaccttga gcagacagag atgaagaatt tgccgattgg tggcgtctgg aaggggaaag
     1381 ttaaattaca ttcgaacagc ccggcccagg actatttcgc aaatattacc ctgaatacgc
     1441 tcgaccccaa ccatattgac gtgttcttcc cggagttcgc ccacgccacg ccaagggtgc
     1501 agttagactt gcatccaaca ggaagcgtta acggcagcaa ctacgcgcaa gatctgacca
     1561 tgttggacat gtgcctgtac gatggtttta acggtaatgc catcagttat gaaatcatgc
     1621 tcaaagatga agggcgacca gctgcagggc gcagagacgg ttacttctct atctatcgtc
     1681 agggagggac caccaccgac gagggagaac gcattgatta ccgggtcaaa atgtacaacc
     1741 cggaaaccgg tgggcaaatt gatgtgcgca ataatgaaaa tatggtctgg aacagcatta
     1801 acctgaaacg tgtgcgtccg gtggtactgc ccggtattcg ctatgccgtg atgtgtgtgc
     1861 cgacaccctt gactctggca gtcgataaat tcagcgtgat ggataaacag gccgggtact
     1921 acatgggcaa attgtcagta atctttacgc cttccttgcc aaccatcaat taaccctgca
     1981 aagcggacaa acgctttttt ctatataaac gacgaagccg tctgataaag cggcttcgtc
     2041 attacaactt aagttaaacg ttaatgttgc ggtgcgggat cgttatgtgt ttcctgcgcc
     2101 acctcaatcg ccggtttgtt ctgcaacacg gtccagataa ccagcgcacc taacaggtca
     2161 aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag tgcaccgaca
     2221 accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc gttcgccgtc
     2281 gccacttcgt tacgaccgaa cacgtcagaa gagagcgtaa tcagcgcgcc agacagtgcc
     2341 tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt ggtgaacaga
     2401 ccgatcatac ccgggccaat catcagcaca gcacccagcg ttacgaccat cttacgggaa
     2461 acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc accgaggata
     2521 caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc tttcaggtta
     2581 aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc ccaggccggt
     2641 tctgccagga aacgcggcag cgcgataccc cagaactgac ggttacgcag gatctgacca
     2701 acggacattt tcttcgccgt gctcacctgg tgctgggctt cctgaccatt aataatatag
     2761 tcgcgttctt catcggtcag atgcttctgg tcgcgcggat gtttatagaa aatcagccat
     2821 gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg ccagctgtgc
     2881 attacgattg cccataccac cagcggcggc gcaatcatcg caccaatcga agaacctacg
     2941 ttaaagtaac ctaccgcgat ggaacgctct ttcgccggaa accattcgga gctggctttc
     3001 agacccgccg ggatcatcgc tgcttccgcg gcaccgactg caccacgagc aatcgccagg
     3061 ccaccccagc taccagccag cgcggttgca ccacagaata cggcccacag cacagcaaac
     3121 attgcataac cgattttcgt acccagcaca tccagtacat agcccgctac cggttgcatg
     3181 accgtataag cagcagaata ggctgcgatg atataggaat actgttgggt ggagatgttt
     3241 aactcttcca tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag gtaaccaagc
     3301 acggtgccaa gcgtcaccag tgcgatcata taccaacgta accctttaat tttacgcatg
     3361 taaaacctca tcgttttgtt atatccgcag gaagcgcggc catcatcccg ccagaggctg
     3421 aagatgttag ttacgaccgg gtaaccgaaa aaaaaccggc acgccccgaa cgttgccatg
     3481 aaagtttcgt gctaattcgg cttccgtacc ggttaacccg attcatctaa caaatgaatg
     3541 aatcggcaat ttgcattccg tcgacttatg atttgcgacg gcagaaagat aacttgtcat
     3601 acaactttaa aaggtgagag ccatcacaaa tgtgcgaata tttgtaggga cattacctga
     3661 cgacagcgag gccagtactg gcgcggcctg cagcgagatt taccactttg agagtaattt
     3721 ttttaactac gtttattgat ctaactcacg aaaatatctt cggactctgg aaattggtgt
     3781 gataactttg tcagcatcgc accataagca agctagctca ctcgttgaga ggaagacgaa
     3841 aatgactccg tttatgactg aagattttct gttagatacc gaatttgccc gccgtctgta
     3901 tcacgactac gcaaaagacc agccgatttt cgattaccat tgccatttgc cgccgcagca
     3961 gattgcggaa gactatcgtt ttaaaaacct gtatgacatc tggctgaaag gcgatcacta
     4021 caaatggcgc gctatgcgta ccaacggtgt ggctgagcgt ctgtgtactg gtgatgcgtc
     4081 tgaccgtgaa aaatttgacg cctgggcggc gactgttccg cacactatcg gcaacccgtt
     4141 ataccactgg acgcacctcg aactgcgtcg tccgttcggt atcactggca aattgctttc
     4201 tccgtcaact gccgatgaaa tctggaacga atgtaacgaa ctgctggcgc aggataactt
     4261 ctccgcgcgc ggcatcatgc agcagatgaa cgtgaaaatg gtcggcacca ccgatgaccc
     4321 gatcgattct ctggagcatc acgcagagat cgccaaagac ggctctttca ccatcaaagt
     4381 gctgccgagc tggcgtccgg acaaagcctt taacattgaa caggcgacct ttaacgacta
     4441 catggcgaag ctgggcgaag tttccgatac cgacattcgc cgctttgctg acctgcaaac
     4501 tgccttgacc aaacgtctgg atcacttcgc cgctcacggc tgtaaagtgt ctgaccatgc
     4561 gctggacgtg gtgatgtttg ctgaagcgaa cgaagcggaa ctggacagca ttctggcgcg
     4621 ccgtctggct ggcgaacccc tgagcgagca cgaagtggca cagttcaaaa ctgcggtact
     4681 ggtgttcctt ggtgccgaat atgcacgtcg cggctgggta cagcagtacc acattggcgc
     4741 gctgcgtaat aacaacctgc gtcagttcaa actgctgggg ccggatgtag gctttgactc
     4801 catcaacgac cgtccgatgg cggaagagtt gtctaagctg ctgagcaagc agaacgaaga
     4861 aaacctgctg ccgaaaacca ttctctactg cctgaacccg cgcgataacg aagtgctggg
     4921 caccatgatc ggtaacttcc agggcgaagg tatgccgggc aaaatgcagt tcggttccgg
     4981 ctggtggttt aacgatcaga aagacggtat ggaacgtcag atgacccaac tggcgcagct
     5041 cggtttgctg agccgctttg tcggtatgct gactgacagc cgtagcttcc tgtcatacac
     5101 ccgtcacgaa tacttccgcc gcattctgtg ccagatgatc ggtcgctggg tggaagcggg
     5161 cgaagcaccg gcggacatca acctgctggg cgagatggtg aaaaatattt gctttaacaa
     5221 tgcgcgtgac tacttcgcca ttgaactgaa ctaaggtctg gggttgatat gcaatacatc
     5281 aagatccatg cgctggataa cgtcgcggtc gctttagcag atttggctga aggcacagaa
     5341 gtcagtgtcg ataaccagac tgttacgctg cgccaggatg ttgctcgtgg acataaattt
     5401 gcgttaacgg atatcgcaaa aggggccaat gtcatcaaat atggcctgcc gattggttat
     5461 gcattggcgg atattgcggc gggggaacac gtccacgccc acaatacgcg cacgaatctg
     5521 agcgatctgg atcagtatcg ctatcaacct gattttcagg atctgcctgc gcaagcggca
     5581 gatcgtgaag tgcagatcta tcgtcgcgct aacggcgatg tcggggtgcg taacgagctg
     5641 tggatcctgc caaccgtggg ctgtgtcaac ggcatcgcgc ggcaaatcca gaaccgtttc
     5701 ctgaaagaga ccaacaacgc cgaaggtacc gacggcgtgt tcctcttcag ccacacctac
     5761 ggctgctcac aactgggcga cgatcacatc aacacccgca ccatgctgca aaacatggtg
     5821 cgccacccga acgcgggcgc agtgctggtg attggtctgg gctgtgaaaa caaccaggtt
     5881 gccgcattcc gtgaaacgtt gggcgatatc gatcctgaac gcgttcattt catgatctgc
     5941 caacagcagg atgatgagat cgaagccgga atcgagcatt tgcatcagct gtataacgtg
     6001 atgcgcaacg ataaacgcga gccaggcaaa ctcagcgaac tgaagtttgg tctggagtgc
     6061 ggtggttctg acggtctttc tggtattact gctaacccga tgctggggcg tttctctgac
     6121 tacgtgattg ctaacggcgg tactaccgta ctgaccgaag tgccggagat gtttggcgca
     6181 gagcagttgc tgatggacca ttgccgcgac gaagcaacgt ttgaaaaact ggtcaccatg
     6241 gtcaacgact tcaaacagta ctttattgcc catgaccagc cgatctatga aaacccatcg
     6301 ccggggaaca aagcgggcgg tatcaccacg ctggaagata aatcccttgg ctgtacccag
     6361 aaagcgggtt ccagcgtcgt ggttgacgtg ctgcgttacg gcgagcgtct gaaaacgccg
     6421 gggctgaact tgttaagtgc gccgggtaac gatgccgtag cgaccagcgc cctggcgggt
     6481 gcgggctgcc atatggtgct gttcagtact ggtcgtggca cgccatacgg cggctttgta
     6541 ccgacggtga aaatcgctac caacagtgaa ctggcggcga agaaaaaaca ctggatcgac
     6601 tttgacgcgg gtcagctgat ccacggtaaa gcgatgccgc agttgctgga agaatttatc
     6661 gacaccatcg ttgagtttgc caacggtaaa caaacctgca acgagcgtaa cgacttccgc
     6721 gaactggcga tcttcaaaag cggcgtaacg ctataaagtc gaaatgagtt gttaataaaa
     6781 aacggcgttt cataatgtga agcgccgttt gcttttccat acctgctaag gatttatcat
     6841 gaccgcgtat tggctggccc agggcgtggg tgtcatcgcc tttctgattg gtatcacaac
     6901 atttttcaat cgtgacgaac gtcgcttcaa aaagcagctt tcggtctata gcgccgttat
     6961 tggcgtacat ttttttcttc tgggcaccta tcccgctggt gccagtgcca tccttaatgc
     7021 cattcgtaca ttgattacct tacgcacgcg cagcttatgg gtaatggcga tttttattgt
     7081 gctgactggc ggaattggcc tcgcgaagtt ccatcatcct gtcgaactat tgccggttat
     7141 cggtacgatt gtcagtacct gggcgctgtt ccgctgtaaa gggctcacca tgcgctgcgt
     7201 gatgtggttt tcaacgtgtt gctgggtgat tcacaacttc tgggcggggt cgataggcgg
     7261 cacaatgatt gaggttagtt ttctgcttat gaatggcctg aatatcattc gtttctggcg
     7321 gatgcagaaa aggggaattg atccgtttaa agtagagaaa acccctcccg ccgtagacga
     7381 aaggggttaa acaattaatt acgcagggcg ctatttgcca gacgatcgtc ttccgcctgg
     7441 caagctgccg cagtgaacag cacgtcagtt gaagagttca gcgcggtttc gcaagagtcc
     7501 tgcaatacgc cgatgataaa gccgacggca accacctgca tggcgatatc gttcgaaata
     7561 ccgaacatat tacaggccag cgggatcagc agcagagaac cccccgccac gccggatgcg
     7621 ccacaggcac acagagaagc caccacgctc aacagcagcg ccgtgggcag atcgaccggg
     7681 atacccagcg tattaaccgc agccagcgtc aacacggtaa tggtgattgc tgcgcccgcc
     7741 atattgatgg tggcacccag cggaatagaa acggaatagg tatcgcgatc cagattcagc
     7801 ttttcacaca gtgccatatt caccggaatg ttcgccgcag agctgcgggt gaagaaggca
     7861 tacacgccgc tttcgcgcag gcacagcagc accagcggga acgggttacg acgaattttc
     7921 caccacacca gcaatgggtt aaccaccagc gccaccagta acatacagcc aaccagcacg
     7981 accagcagtt gcgcgtagcc ccacagtgtg gagaaaccgg tggttgccag ggtagaagaa
     8041 accagcccaa aaataccaat cggtgcgaag cgaatgacca gtttcaccat aaaggtaacg
     8101 gcattcgaca tatcgttaac caggtttttg gtggtctcgt taccgtgacg cagtgcgaag
     8161 ccgaggccga tcgcccacac cagaatcccg atgtagttac ctttcagcag cgcgtcgatg
     8221 gggttggaaa ccatgctcat taccagcccg cgcatcactt cgacaatgcc tgacggcggc
     8281 gaaatatcac ccgcgctact ggataaatgc agggtagaag ggaaggcaaa gctgaagact
     8341 actgcggcca gagcagcaga gaaggtgccc agtagataga ggaacaaaat agggcggata
     8401 ttggttttct gcccgtgctg gtggttagca atagatgcca ttaccagcat caacaccagg
     8461 atgggggcaa cggctttcag tgcgccgacg aacaaagtac ctaacagacc aacagcttcc
     8521 gccgcgggtt ttgagatcca tgccagaaga atccccagaa caaggccgac caggatttgt
     8581 tttaccaggc tgccatgagc cagacgccgg aatagccccg gtgaacgttg cgtagtcatt
     8641 tttcgatcct ttcattgtgt tgtcgcacat ccctggtgcg ttctgtcgac gcatctttca
     8701 ggatgtaaca aagtgtttgc aaggtcgagt ataaggaaag ttacagcttc gggaagcgaa
     8761 aaatgctgga ttttaaagag aggtcatatt ttgcaactta atggttacat ttatttgaca
     8821 taaaaataaa gccaacgtgc ggcttccaga ctgatgacaa catcaaaaaa ggactcggtc
     8881 agtcccctcg ccccgtttgg ggagagggtt agggtgaggg gaacaggcca gcatcggtgc
     8941 gaaaaattac cctcaccccg accctctccc tggaagggag agggagaaaa cggtgctcat
     9001 gttaatgaca gggtacacgg cttaggcaga ttaaaaatta tccaccccgc tgcttatcat
     9061 gccgataatt cacccaggcg ttgataataa acgtcatcac cagaatgcca aacaccacgc
     9121 ccagcgagac ggcgattgga atatggtaga agtcgacaat cagcatcttg ataccgataa
     9181 acaccaggat caccgccagg ccatatttga gcatcgagaa acgctctgct acgcccgcca
     9241 gcaggaaata catcgcacgc aggccgagga tcgcaaacag gtttgaggtc agcacaatga
     9301 acgggtcagt ggtcacggcg aagatagccg gaatgctatc cacggcgaaa atcacgtcgc
     9361 tcaattccac cagaatcagt accagcatca gcggtgtggc gtacagcaat ccattcttac
     9421 gcacaaagaa atgctcgttg tcgatggtgt cggtcatgcg caaatgaccg cgtagccagc
     9481 gcaccagcgg cttgtcgcca atgcctgatt catcttcatg ggcgagcgcc atcttcacgc
     9541 cggtaaacag caggaaggca ccgaagatat acaggatcca gtcgaactgc gaaattagcc
     9601 agctgccagt gaagatcatg atggtacgca gaacaatcgc cccgagcacg ccatacacca
     9661 gcacgcggcg ttgtaatgcc gccggaacag agaaatagct gaacaacatc agccagacaa
     9721 agacgttatc gaccgccagc gatttctcaa tcagataacc tgtgagaaag gccagtgcct
     9781 gtggatcggc aacggcgcga ccttcggttt gcaccagata ccaccagaaa gcggcgttaa
     9841 acagtaacga cagcgtcacc cagaccagcg accaggccgc agcctgtttc atggtcatgg
     9901 catgtgcccc acgacgcccc tgcaacaaca ggtcgatagc cagcataatg gcgacaacaa
     9961 cagcgaatcc gccccatagc aacggcgtgc cgacagtatt catagaagtt ccttacacat
    10021 aaaaaaacgg ccaacgtcgg aagacgtcag ccgctgcttt ttatgcatag acctcgcctt
    10081 ccggcaaggt ctcacttaca acaaaaaagg aatacgtctg cgtattcctt tcgggttgcc
    10141 cggtgaccgg atgtggtttt tcacacatcg taatgacgat cgaccggcaa tgaagttact
    10201 cccctttgca ggtaacaaag tatgacagac cattcagcca gtcaatggtc tgttacatac
    10261 cttttacact gttttacttt ggcaattata gttttacgct atctgccgga aaaatcaccc
    10321 cagtctggcg gcggatctcg gtcagcagtt tggcggtgat gcgactgacc gccagccccg
    10381 gatgatccac cagatgctca tccaccaggg tagcgaacag ctctgcttcg tagagcatgg
    10441 tattaatatg ctgcggctgg gtgagatcct gcatttggct gccacgcggc acgaagcaca
    10501 ctttctggca ttcagacagt ttttcaatca ccagcgatcc tgcttcgccc tgaatctcgc
    10561 tcgccaggac agaatcactg acttttgagt gctgcaaggt gacgctgaaa tcaccgtaat
    10621 ccatcaccac cacgccgtgg gcatctacac cgcttgccag caaactggcg gttgcctgca
    10681 cgcttttcgg ctcaccaaat aacgccaccg ccgacgccag gcagtaaaag ccgatatcca
    10741 taattgaacc gttagagaat gacggattaa aggtgttggg attttcaccg tccaggtaac
    10801 gttgatagcg cgaggaatac tggcaatagt tgaaaaagac tttacgcaat ttgccgactt
    10861 tcggcagcgc ctggcgcagc aaatgaaagt tcggcaggca ggcggtttta aatgcctcaa
    10921 acagcaccac ctgattttcc cgcgcacagg caatggcggc atccacttcc gccagattcg
    10981 acgccagtgg tttctcgcaa atcacatgaa ttttatggct aaggaaaagt tgtgtctggg
    11041 aaaagtgcag ggcattcggg ctggcaatat acaccgcgtc aatggcatcg ctttccgcca
    11101 tcgcttccag cgaggtaaac agatgctcga cagaaaaatc attggcgaag tgctgagcct
    11161 gctcaaggct gcgggaatat acggcggtta acttgtattt accgctctca tgggcggcct
    11221 cgacgaactg gcgagtgatc cagttcgtac caatcacagc gaaacgtatc ataacgcgtg
    11281 gatactccat aaagggattc tgtcgtcaat gtagcacgcc tttttcatct tcgacgtgcg
    11341 cagatacgca ttattctttt gccggacagc catgctcact aaacggccca atcatgaccg
    11401 atggcccgag atatttcggc tggcgatgga ggattttggc gtcaatcacg gcgctgacac
    11461 gagcgagttt ctcccgcagt tgagtataaa aaccgtgctt catattgagg tcaggcgcgt
    11521 taaaaccgat agcgtcgatg ccgtactgtt ttgccagcca gatggcgcgt tgattatgaa
    11581 attcttgcga gataatggtg atatggtttt caccaaaaac ctttttggca cgcactaccg
    11641 aatccagcgt tgagaatccg gcatagtcgc agaagatcac tttcgccggt acgcctttag
    11701 cgatcaaagc ctgctgcata cctgaggctt catcgtaatt tttacgtccg ttatcgccac
    11761 ttaccagtag ccatttcact tttccggcgt ggtacaacgc tgctgcggta tcaatacgtc
    11821 gggtaaagta gcgattaccc ggcctggccc ccagcaataa gcccacgtta cgcgccggaa
    11881 ctgcgttgac atcgctccag gtcagctgct tgctggcgtt gaccataacg cgatcggcaa
    11941 taaaaatggt cgctcctgct ataagcaaca acaaacagcc aatcttaaga gtccggcgag
    12001 aaaaacaaat acggagaaga aggcggggct gcatggtcgt tatccttaac gaaagggggg
    12061 aggtcgattt taggcgctaa agaaagaaaa gataacgcct gacatcattt tcagattttg
    12121 tcctgcccaa gcgacaactg cgttagccac agccgggtgt cgaactcaag ctggtggtac
    12181 tgcggctcca tatggcagca caactggtaa aacgctttgt tatgctcttt ctccttcagg
    12241 tgcgccagtt cgtgcaccac gatcatacgt aaaaacggtt ccggcgcgtt gcgaaacaca
    12301 gtagcgacgc ggatctctgc ttttgctttc agcttgccgc cctgcacacg agaaacagcg
    12361 gtatgtaacc cgagcgcgtt ttttagcaca tggatcttat tgtcatacat cactttattg
    12421 atcggcgggg cattgcgcag aaactgattt tttagatcct gagtatattg ccagagggct
    12481 ttatcggtgg cgtagtcgtg tgttcccgga tagcgttttg ccagcacatc gcccagacgc
    12541 tgttcgttaa tcagcgttcg cacctgggaa agtagctgct cgggataacc ctgaagataa
    12601 gtaagattgc tcataaccgc ccacgaaaaa gagaaaaagg gtatactcac gcaccctttt
    12661 caggggcaac gtcgaaattt tatcattcag gagggccgat gagccactta gacaacggtt
    12721 tccgttcact gacactacaa cgttttccgg cgacggatga cgttaacccg ctacaggcgt
    12781 gggaagcggc ggatgaatat ttgctgcaac agttggacga cacagaaatc cgcggcccgg
    12841 tgttgatcct gaatgatgcc tttggtgcgt taagctgcgc gctggcggaa cataagccgt
    12901 acagcattgg cgactcatac atcagtgaac tggcgacgcg cgagaattta cgcctcaacg
    12961 ggattgatga gtcgagcgtg aagtttctcg acagcaccgc cgactacccg caacagccgg
    13021 gtgtggtgct gatcaaagtg ccgaaaacac tggcattgct ggaacagcaa ctgcgtgcgc
    13081 tgcgcaaagt ggtcacgtcg gatacacgta ttattgccgg tgccaaagcc cgtgacattc
    13141 acacttccac gctggagctg ttcgagaaag tgctcggccc gaccaccact acgctggcat
    13201 ggaaaaaagc gcgcctgatt aattgcactt tcaatgaacc gccgctggcc gatgcgccgc
    13261 agaccgttag ctggaagctg gaaggtactg actggactat ccacaaccat gcgaatgtct
    13321 tctcccgcac cgggctggat attggcgcac gcttctttat gcaacatctg ccagagaatc
    13381 tcgaaggtga gattgtcgat ctcggctgtg gtaacggcgt tattggcctg acgctgcttg
    13441 ataaaaaccc gcaggcgaaa gtggtgtttg tcgatgaatc gccgatggcg gtcgcttcca
    13501 gccgaatgaa tgttgaaacc aacatgccag aggcgttgga tcgctgcgag tttatgatca
    13561 acaacgcgct ctccggagtg gagcctttcc gctttaatgc tgtgctctgc aacccgccgt
    13621 ttcatcagca acatgcgctg accgataacg tcgcctggga gatgttccac cacgcccgcc
    13681 gctgcctgaa aatcaacggc gaactgtata tcgttgccaa ccgtcacctg gattacttcc
    13741 ataaactgaa gaagattttc ggcaactgca ccaccatcgc cacgaataat aaatttgtgg
    13801 tgctgaaaac agtgaagctg gggcgtcgtc ggtaagattg ggagttacct gccggatgcg
    13861 gcgttaacgc cttatccggc ctacgttcag cacgttactc ccaggccgga taagacgcgg
    13921 caagcgtcgc atccggcaat gtctgcccag ttctggaatc gcgcttccat aaaacaaaaa
    13981 atccctgtca ccatcctcta cattctcttg tttagcgttg ttcaacgttt atgctacata
    14041 cgtgaagtta tgacgcataa agaatacgat ttctttaccg ctgttcatcg tactaagggg
    14101 aaaaaatgat tgcgattgcc gacatcttgc aagcaggaga aaagctaact gcggtggcac
    14161 cttttctggc gggtattcag aacgaggaac aatacaccca ggcgctggaa ctggtagatc
    14221 atctgctgct caacgatcct gaaaacccct tgctggatct ggtgtgtgcc aaaataaccg
    14281 cgtgggaaga atcagcgccc gaatttgcgg aatttaatgc catggctcaa gccatgcctg
    14341 gcggtatagc cgtgattcgt acccttatgg atcaatatgg tttaaccctt tccgatctgc
    14401 cggaaattgg cagtaaatct atggtgtcac gcgttttgag cgggaagagg aaattaacgc
    14461 tggaacacgc taaaaaattg gcaacgcgat tcggcatttc tcccgccttg tttattgatt
    14521 aacgcgacgt gccggatgcg ttgcatccgg cgcttcaaat aacgatttaa atctccagcg
    14581 ccagccgtgt tccctgggca attgcccgtc gtgcgtccag ctccatagcc acatcgcagc
    14641 cgccaattaa atgcacagtt ttcccgctat caatcagcgg ttgcgccagc gcgcggtttg
    14701 gctcttgccc tgcgcagatc accacattgt ccactgctaa tacctgcgtt tcgccgttga
    14761 tcaccacatg cagcccgtca tcgtcaatct tctgataact tacgcttggg atcattttca
    14821 caccccgcga gagcagggtg gtgcgatgga tccagccggt ggttttgcct aacccctgtc
    14881 ccggtttgct ggctttgcgc tggagcatca caatttgccg tgggctacgc gggatctgca
    14941 ttccctgcgg gcttaagcca ccagcctgtt gtaggctgct gtcgatcccc cactcattac
    15001 agaacctggc gatattctgg ctggtggatt cgcccggctg acttaaatac atcgccgtat
    15061 caaaaccaat cccgccacaa ccgatgatgg caaccttgtt gcctaccggc gctttgtcgc
    15121 gcagtacatc gagataactc aataccttcg gatgatcgat cccgtcgata ggcggaacgc
    15181 gcggcacgat cccactggcg aggatcgttt catcgaacgc ctgtaactga tccgccgtca
    15241 cggtgtgatt gagtttcagc gtcacgcccg tcacttcgat catccggtgg taatagcgca
    15301 gcgtttcgta aaactcctct ttgccgggga tctgtttggc gatattaaac tgcccgccga
    15361 tctcgctgtg ggcatcaaac aatgttacct gatgcccacg cgccgccgcg ttaatggcaa
    15421 acgccagccc agcaggtccc gcaccgacca ccgccagatt ttttttctgc acggcaggaa
    15481 ggattggcat tttggtttcg tggcaggcgc gaggattcac caggcacgag gtgactttgc
    15541 caacgaagat ctgatcgagg caggcctgat tgcagccaat gcaggtgttg atctcatcgg
    15601 ctcgccccga ttgcgctttt gacagcagtt ccgcatcagc aagaaacggt cgtgccatcg
    15661 acaccatatc ggcatcgccg cgcgagagaa tgtcgtcggc aacctgcgga tcgttaatcc
    15721 ggttggtggt caccagcggc agcgagacgt ggcctttcag tttgcgcgtg acccaagtaa
    15781 atgcgccgcg cggcacgggc gtggcaatgg tcggaatacg tgcttcatgc cagccaatgc
    15841 cggtgttgat aatggtcgcg cccgccgctt caatggcctg cgccagttct accgtttcgg
    15901 caaaagtgcc gccgccttcg accaggtcga gcatcgacaa ccggtagata ataatgaagt
    15961 cgttgccgac gcgttcgcgc accgcacgca ctacttctac ggcaaatcgc atccggttgc
    16021 ggtaatcgcc gccccactgg tcactacgct gattggtgcg cagcgtcaga aattcgttga
    16081 tcaaataccc ttcggaaccc atcacctcta caccgtcgta tcctgcctcc cgcgccagtt
    16141 gcgcgcagcg ggcgaaatcg tcgatcagtt gcaggatctc ttcatgggtg agttcgtgag
    16201 gaacgaaacg gttgatgggg gcctgaaatg cggagggagc gaccagatgc ggttggtagc
    16261 tgtagcgccc ggtatgcaaa atttgtaagg caattttgcc gccttcctga tgtacggctt
    16321 cggtaatggt gcgatggtgt gggatctggc tggcgtcatt gagcattgcg ccaccttcca
    16381 tgccgacgcc tgttaaatct ggtgcaatgc cgccgctaac aatcagcgcc acgccgtgac
    16441 gggcgcgttc ggcataaaac gcagccagcc gttcagcacc gtccgggtat tcctccagcc
    16501 cggtgtgcat tgaacccatc aacacgcggt tttttaacgt ggtaaaacct aaatccagcg
    16561 gggcgaacag cgacgggtag ctcataaaat tgtccagtat gtaaaataat tgttatgtgg
    16621 tcggatgagt tctaatttag ccgtcgccgg agaaaaggga aaaggggagt gcggggtttg
    16681 tgatgggatt caaaaagaat acgcccggtg gtaacgccgg atgcgacgct tgccgtgttt
    16741 tatccggcct gggggattgc acatgtaggg cggataaggc gttcacgccg catccgccag
    16801 tggcgcggtg cagttgccgg atgcaacgct tgccgcgttt tatccggcca ggaggattgc
    16861 acatgtaggg cggataaggc gtttacgccg catccgccag cagttgcaaa ttattgctta
    16921 cggaaaaaat cgttatacaa catatacaaa tgcgcggcac tccaggagaa atttggtgcg
    16981 ccttgctgtg cgcctgtcag cgggttgtaa ttttcctgaa ttggtccatc ggcggttaac
    17041 cctttggcgt gccggaagaa cgtatccgcc agcttcaggg catcatcacg ataaccgtaa
    17101 cgctccatcc ctttcagacc aaaccagaac tgatccaccc atacgcgccc acgccagtag
    17161 atatcagcgc caaaagccgg attggttaac gccgccgttc ccagcgggac aaaggtgttg
    17221 aactctttag gatcgagcat caccttcacc accgcgtcgg cgttggcctg cgttgccgca
    17281 ccgttaaaca gcggcgacca gccttccggc cctttaccgc gctcaacaat cggtttgccc
    17341 gcgcagccgt tcgccagcgg tttatcttca atacgcacgt catagtagaa ctgggtagtc
    17401 gggtcgaaca tacaggtgtt gatgtagtcc gcgagctgct gtgccaactg gcgatagcgt
    17461 ttggcctctt ctggcttacc gaggatggtt gccatctccg ccagataatg gttatcgctg
    17521 tacatatagc tggcctgatc caccgactcc tgcaatagcg agtagcccag caacgttccg
    17581 tcctgactgc ggttttcggc gaatttcacc atccagtcgc tacgtttgcc gccattagcg
    17641 acatatttat ccaactgttc tttgtcgata aacccaaaga cggcggcatc atcacgcccc
    17701 gattcccacg atgcagcaac ctgtgccgga atttccagac tgtcgtactg gcctttctcc
    17761 accacgcggg cgtagttgtt cagcccagac tgcgtctctt ctttatcgcc ttttttcacc
    17821 gtaaacagca tctcgccgct ctcagtgttg tgggctttgt cgcgggtcgc gccatattcc
    17881 ggcacgccgt tgccgttgtg atcgcggtta cgtaaccacc agtcgtgata ggccaccagt
    17941 ttcgggtaca tctctgccac ccaggtttta tcttgggtga cgttatacac ttccatcacc
    18001 gaccaggcgg caaggctggg tttggtatta cgttcgttcc agttgccgcc atcgccacca
    18061 cgctcggggc taaggttcca cgctatcagg tcggggacaa agcccacatc ctgcggacgc
    18121 acgcggtcgc ctggctggat ctgccaggag aagaccgcgc ggatattctc tttggcgatg
    18181 tccggattga aatgcgccat cgcaaacgcc tgcttccagg tatcccacgg ccaggtctga
    18241 ttgccggaga accagcgccc ggtcaccgac ggtgtgacgg tgttaaattt caccgccccg
    18301 ccaggcgaac gccagttacc gttgagcgtt tcgatcgctt tcaccgcgac gcgcgtctgt
    18361 tccggcgtcg catccggatt ggttaaccct ttcttcagat actcttccca gcgttgctgc
    18421 gaggcggtga gataaaacgc cggacgcgcc agaatatcgc ggatctgcat ttgctctttg
    18481 ctaacttcct gagaggtcag cagatgggaa taggtggtat agagcgtcgt cgaaccgttg
    18541 atatgtgcct tactggtaaa gcgattgccg ttgatttcag tctgcaccgg cagggattta
    18601 tgcacctgat attctgattc accggaggtc agcagatccc aggtggcgcg cactttgcca
    18661 aaggtgactt tcaggccatc acgggtggcg ctgattttgc gctggtagtc agggtattcg
    18721 ccagcaatgg ttttatcgga aagcggtttc ccttctttcg cttccagttt ttccagcagt
    18781 tcgccatccc acaccagatc cagcggttta ttgctgatga ttttagtttc cagtagtgac
    18841 gtgcgcggcg tggcgaagcg cagagtcatt tcgacctgca catcttttgc tgtcagtttt
    18901 tgcaccagcg cgccgggaat actgtatgcc tccagcgtga agtcgacttt cttgccgtcc
    18961 tgccagacgg tcaggtggtc gaaattgctg gccataaagt tgatgtactc ttccgtcagc
    19021 agcgcaacgc ctggaaaacc gcccatggtg ttagggccgt ctggcaacag atgaccatgc
    19081 caggctccga gatcgaaaaa cggattaaaa cgctgatgat cgtcgtaatc gtaatccttc
    19141 atgtactgcg gcgcaccagt acggttaatc acgtttttat aattgtcggc gttagcggca
    19201 tgggcggaaa aacttatcag cagagcgcag gttactggcg ttaaaatagt tttgattttc
    19261 atgaaattac agtccttatt accagtagaa atattgcatc aggaagattt ggtcgctggg
    19321 atcgtgagag gcatttttca tgtagaagcg gctatcaaaa gccgttgcga aacgtccgcc
    19381 gccgtattcg taccagatac caaactgcat gaaagaggtg gtttcatcgt cagcgtggtc
    19441 agggcgatgt ttggcgtagg tgtaagcggt ggagagataa acgcccttta atgcttcata
    19501 catggcgctc accatcatcc cactttcact tcccggtacg tccagcccgt cggcacgtcc
    19561 ggtatggtgc caggcgcgag cggcaaagtt aggggtaagc aggccggtga gatagagttg
    19621 tcctgtgccg tcggtaattt ccagaccatt catccaggtg atgccttcgg caaggtcata
    19681 ctggatgtaa ccgttcatca ttgccggata ggtgtatttg tcgtcgtagc gatcgtactt
    19741 gccaaaatgt agccaggctt tgctttcgtc atgatgtccg gcaggtgtgg cggtgacgct
    19801 ataacgaaag ttgccgctta agttttgcac cttcagcgca gtcataatgt cgcgggtatt
    19861 gggaatgacg tagcccagat cgggcgtgaa atcgccccac cattgcaggt catccagcga
    19921 ggaatcctga cgcagactga gcatgatttc ggtgccatca ttggtacgat aaccgccata
    19981 gaaacggtta attccgccct cgaaaccacc ccagctatgg tcaggcaccc aggcattgcc
    20041 gtcgtggtcg gcctgcacgg tccagccttc gccgtaaatc aggccgaacc aggagccgta
    20101 cttcatctcc aggccacctt caatataggt gccgtcactg tatttatgtt tattctcacc
    20161 ctctaaatcc atatatccac caacccctaa ttcgccgtaa aaacgaaatg aaggagaaga
    20221 tgtcgaggta atggcgggtg tttttgtgtc gtcatgttct gccggacgtg cttcttctcc
    20281 tgccgcagaa aaggcgtagc aaaacggcag agcaagtaat gctctgcatg gtgcagtgat
    20341 taatttcatt gtgttaatcc atgagctaaa gaaaaagtac agccttttat cgttgtaatt
    20401 ataaaaatat aaaggcagaa actattttcc ctctcctcat ccaggaagag ggaaaatgcc
    20461 tgctcatttt ttattattct gcttaatcaa cattgctggc tggcgtcgct tcaatatggc
    20521 gctcttcagc cgttaatccc tttatatatt tactgtattt ccaccacgca aagccaagga
    20581 agataacaat accgccgacg ttataaaaaa tgatggtcag aatattcgcg ccagtcggga
    20641 atgtcgaagc gacaaaccct acggcaaata tcgcaatcag cattgaaaca acgataatac
    20701 cggtgcggcg ggagcccata cggaaatcgc gtggcaggtg atctaatttg gcgcgtaaat
    20761 tcaggtaagc cagcatgatg aataacggcg gaagcatgga cgctgcggca gtcatattaa
    20821 taatagtatt catcagatcc tgcacggtat tggaaccgag catcgggata atcatcagcg
    20881 ggatgacgat caggaactgg atccacgctg cgcgcgccgg aacgccgttt tcgttcagtt
    20941 cgacggtttt cttaccaaag atgccttccg ggatttcgga gaagaaaatt ttcaccggcg
    21001 ttgcggtcca catcagcagg gaaccgaaca tcgcggtaaa ggacaccaga ccgacaaagc
    21061 gattcatcaa cgcttccggt agaccaaaat acgccgccat gccgtggaat acctgcaccg
    21121 atccgccggt aaatttcaac tctttgctgc tgacgaagac gttaatcagc accgaggaga
    21181 cggaatacag tacgccgata aaaatcccgg cgaggatgat cactttaacg aacgatttcg
    21241 aaccgccttt gacgtcgtta acgtacacag cgacggactc cgcgccacct gcggccataa
    21301 agatccaggt ggtaacgccg aggaacgccc agttgaagtt cgggatcatc gcgtcaacgg
    21361 tgatggggtc agcaggctgt acgccgccaa ccagcgccgt acctgccagt aaaatgtagg
    21421 agagcgttaa cagcagcatc agcgttgaag tgacggaggt aattggcccc aacattttcg
    21481 ccccgttggt ggaaacccag gtggagaagg cgaacagcac catactgata atggtggtgg
    21541 caaccggcgt cataatatat tcgtagccga ggaaggcata cgaagcataa gcaataacgc
    21601 gcggcaacag tgaggtgaaa aagaacaggt ttacgaacca gtaggtatag gcagtaataa
    21661 atgcccaacg tccgcccagc gaacttttta cccacgcgta gacaccggct tctgagtttt
    21721 tatttaacga aacaaattct gcgatgatca gacagaaggg aataaaataa aaaatcgtcg
    21781 cgaggaaaaa catcggtgcc gaggccagtc caagctcaat attattattg ataacgttgt
    21841 taaagctgta aacggcggca aaagtcagcg agagcaagcc gaatttgccg attgtattac
    21901 gtttggtatc agacataaca catctccgaa aatgtgtagg gtagcggtgg cggcaaactc
    21961 cttttgccgc cgtagttgtt atttgttatg gaaataacca tcttcgatgg tgactttgag
    22021 aaccactttt ttgaccgcgt tattgcagat aaaccgatac gcttcatgga tatcgcaaat
    22081 aacgatttgc ccttcgtgga cctcaacggt ttctccgcag ccttttaaat attcacggtc
    22141 agtttcatca cgataatatt ccactacctg taatgtctct ttcggcgcat attcaatttt
    22201 ttgctgccct tgcaggtagt aatgcacttc aaaatagcgt cgatgcccgg taaatagcgc
    22261 atcggtcgcg gagtctgtct ccacgcggta agtcaatgag tcaccgatgg agtgggcgac
    22321 gccaggctga atgttgtcga tattttcaat cgcttcaacg cagcgttgcc acttcttgcc
    22381 agaggcgtaa atctggcgga actgttctaa gttatcgatg atcctcattg cttattctcg
    22441 ctgtgcaaat tcgtggaaaa gaaccctgcg ccgaactcat acgacgccag gctttgcgcg
    22501 gtagcttctc cgccagaaac cggcagcaac gtaaagccgt agctgaagtc acggaaccag
    22561 acgcgccagg agtccagcac ctcgctgccc caggagttgg agccgaggcc aagcagctgg
    22621 tgatcgaggt tcagggtgat gtcatcactg cgctgcagct cgttacagtg ctgggcagcg
    22681 tggatgtttt cctgggtata gtgccaggcg ctgaagttaa ttgggcgctg cggaaccacc
    22741 agcaaaccgt tgccgtggcg gttagtcagt gccgtccagc ggacatgctg acggttgccg
    22801 ttgttctgcg ggaagggata gttctcgaac atggcatcga cggtgctgcg ccagatatcg
    22861 atgatgttag cctgctggct gtcggcgtag ttttcgcccg gtccacgacc gtaatacgcc
    22921 acctgatcgt attcgccgtt aatccccatg gtgaaaccga tgcacggaat gatgtgcgga
    22981 tagtcgccgt aacgctcgcc ggaaagcgcc acgttaacct ggccatcggc agcgatgcgc
    23041 cagatgtagg tgcagcgcat cccgaagtca aacaccggcg gggcaataac tgtgcggctg
    23101 atgatcaaca cttcaccatc gctctgttct acggcaaagt cgcgcagatg ttcctgcatg
    23161 atctgcaaat gattcggttg ccacagccct tcgtactcct gcttgtggtt gtcgatcatt
    23221 ggcttgaaga agttgatctt tggctcgcgg gtcagcagcg actcgccatt cacctgccag
    23281 gatgtcggtt tgccactcat ttttgagaag gtgatcgcga agttgtagcc gcgaacggtg
    23341 cagctcaaac gatcgtcttc cagcgtcagc ggacgcgcat tatttggtgc gaaaggcact
    23401 ggttgcgcgg tgttttcctt cagcgggaac tgataagtgg cgatagaatg cccggcttcg
    23461 ctgtagcggg tgcgggaatc tttggtcacc gtaatgttga ggaacgcttc gcgggcgtcc
    23521 agctgcggca gcgtgatctg caagggggct tcgctgttcg gcgcaacgtc gcgcagttta
    23581 atctgctgcg tcgcgagcgt ttcaccttcg gcgcgcacct ctgcgtgcag ggtgtagtca
    23641 tcaagcgtgg taaaccacag tttattttcg actttcagct cgccgcgagt cagatccagc
    23701 gcgtggattt ttaccggcgc gataacctgt ttgtactctt tcaggcctgg tcccggcgtc
    23761 tgatcggaat agatcaaacc atcaagacag aagttatagt tgttgggata gtcgccgtag
    23821 tcgccgccga atttatacca gacattgccg ttgtcatcct gcgcctggat cccgtggtcg
    23881 caccactccc aaacataatg tccctgaata caatcgtgct tatagaagac gttctggtac
    23941 tccgtcagcc cgcccggtcc gttccccatc gcatgagcat attcacagat gatgcgcggc
    24001 ttcggatgcg ggtattcacc aaactcattc atcagcggca cgcgggtgta catggtggaa
    24061 ataatatcga ccacttcagc atcgcgatct tcttcgtaat gcaccagtcg cgtgtcatcc
    24121 agcgccttcg ctgcgtggta catcgcgcgg atgttacagc catagccgga ttcattgccc
    24181 agcgaccaga tgatgatcga cggatggttt ttctgcgcgt gaatatggcg aataatgcgc
    24241 tcgacgtaga ctttttccca ctgcggatcg tcggtaatac ggctgatatc gccgacatta
    24301 gcaaagccgt gcgattcgac gtcggtttcc gccatcacaa acaagccgta gatatcacac
    24361 agttcgtaaa aacgcggatc gttcgggtag tgagcggtac gcacggagtt gatgttgtgc
    24421 tgcttcatca actggagatc tttctcgacg cgatccattc caacggcgcg gcctttgcga
    24481 tgatcgttgt cgtgacggtt gacgccgtgc agcatcacat aacggttatt gatccagaac
    24541 agaccgtcgc gcactttgat atcacggaag ccaacgcgtt gtggtaccac ttccagaacg
    24601 ttgccgttgg cgtctttcag cgtcatgacc agatggtaaa gataagggga ttctgctgac
    24661 cattgctgcg gctgttcgac agtaaaagca aagctggcgc tggtcagttt ttcaattgcc
    24721 aaatgatcaa tggcgctgct gtgcaccacg cgttcgccat caaacagggt atattccagc
    24781 gtcgtgacga caggggaggc ggagagattt tccagcacca cttcgcagga aagcgtggca
    24841 tcgcaatagg cttcgtcaaa gtcggtacga acggtgaaat cgttaatatg tgttagttgt
    24901 tttccgatca gataaacatc gcggaagatc cccgctgacc accacatatc ctggtcttcc
    24961 acgtaggtag agtccgccca ttgcatcacg cgcacacaca acaggttgtc gccggtttta
    25021 accatcgcgc tgatgtcaaa ctctgcggtc aggcgactgc ccttgctgaa acccacatac
    25081 tgaccgttaa cgtagacttc aaaataggtt tcgacgccgt caaatttaat cagcgtctgt
    25141 ttaccctgcc agccgtcgct gagggtgaaa atacgttgat aggcaccggt tgggttatcg
    25201 ctggggacaa acggcacatc gatggggaac ggaaaacctt cgtcggtata ttgcagtttg
    25261 ccgtgacctt ccatttgcca catggcgggg acggtaatat gcccccagtc agccattaac
    25321 tcagaggtga aggcttcagg cacttgcagc ggatggtcaa aaaagtggaa attccactgg
    25381 ccgcttaagg gcagaaacag gctgctggtt tcgcgggcaa aggtacgcgc ttgcgcaaca
    25441 gaatcatatg aaaaaaagta cgcacgtggc gcaagtcggt tttcgtgggt gagctgaatg
    25501 ttttcccagc gattcatagg gcctcccggt agagagaaat cggaaccatt catggtgatg
    25561 gtagtttgtg taattgctgg ggagtttagt aaaattttta ctaaaaagta gcgggtaaat
    25621 gcatttactt taactggatc acgaaaataa acggcagcac tgtccggcaa aaacgattgc
    25681 cggacagcgt gcaaggggat ttaacgggtc gtgccgcgca gttttaattt gctgggaacg
    25741 aagaccaaca gcggcagcgc gcgaccatcg cgggcttttt catacaccag gttaacgccc
    25801 tgacttccca tcatctcgga atggatgcgc acggtggaga gcggcggaaa ggtaaatcgc
    25861 gcggtgggga tatcgttaac gctgataagc gaaatatcct gtgggatgtt caggccacgt
    25921 tcatgaattg cccgcagtac gccgatagca atggaatcag aagcaacaaa cagtgccttc
    25981 ggatagtctt cctgcgccag cagctgtttt gccagttcat agcccgacga actggaaaag
    26041 cctccgcgcc agatatcctc ttcgcgtacc acttgtttca gtcggccata ttccgcaaag
    26101 gcgacctcac gaatatccgc tttgcctggc tcatcttcac cgccaataaa accaatacga
    26161 ttaacgccct gattgatata gaagtcgatg atttctttac tgatgcgtgc cagatcgata
    26221 tccaccgcat cgtaaccgct gccgggttcg tgaaagtcga taaaacagat attgtcggtc
    26281 aacgcacagg cggcggcgcg cagggcgggc gtgggtttgc cgacaattaa aataccggtg
    26341 acgtttttaa tgtctggtaa tccgctgtgt tcataacagt tggtgagctc aatgcccagc
    26401 ttttcgcact gagtttcaat gccgtggcgg atcgccagat agtaaggatc gttgatctcc
    26461 agctcctgtt ggtagctgta gatagccaga atatggtgtt ggttgactgc gcctgtctgg
    26521 agtttacggg cactactggt cttgtactcc agcttttcgg cgatctcgag aatgcgatgt
    26581 ttcgtctctt ctttcacatt caatgtcgga tcgtcattta agaccctgga tactgtcgcc
    26641 agggatacgc cagcttcgat tgcgatgtct tttagtgttg ccatgtttac cttttttact
    26701 ttccctgatg tgcgccccca ttgtagagga agtgcttatc gggtcacctg ttttcagtaa
    26761 ataaagcgcg tttctttgac gctgtaacgg taacacagcg gctcacgaat gtgatcacgc
    26821 ctgcattctc catttttatc ctacaggatt gagaaaaact tttgctatag tttagtaaaa
    26881 ttttacttga agggaggaag caatggaaac cgtggcttac gctgattttg cacgtctgga
    26941 aatgcgcgtc ggaaagattg tggaagtgaa acgccatgaa aacgccgaca agctgtacat
    27001 cgtacaggtt gatgtggggg aaaaaacact gcaaaccgtg accagcctgg tgccgtacta
    27061 cagcgaagaa gagctgatgg ggaaaacggt ggtggtctta tgcaatctgc aaaaagcgaa
    27121 aatgcgtggt gaaacgtcgg aatgcatgct gttgtgtgcg gaaaccgatg acggcagtga
    27181 aagcgtgttg ttaacgccgg agcggatgat gcctgcgggc gtacgcgtcg tataaaaaga
    27241 tcggatggcg acgtcgtatc gccatccgat ttgatattac gcttcttcga cacttactcg
    27301 catggccgcc agcgccttac gcgccgcttt gatcaccagt tcacactgtt caatggtcag
    27361 tgtcagtggc ggttcaatgc ggatcgtttt ggcgttattg agcgttccgg ccaccagtac
    27421 gcgctggcgg aacatctcgc tggcaaagtt atagccgatt tcgttatcaa caaactcaat
    27481 tgccatcaac atccctttac cacgcgcttc ctgtaccaga tcgggatatt cccgcgccag
    27541 ttgacggaaa ccgtccagca acatatcgcc tttttgctca gcctgagccg gtaagttctg
    27601 ctccagcaac acattgatgg tcgccagcgc cgccgcacag gccagcgggt tgccgccaaa
    27661 ggtggtggta tgcaggaatg ggttgtcgaa cagaactgaa aacacctctt cagtggcgat
    27721 ggtcgcgcca atcggcatca cgccgccgcc gagcgctttg gcaaggcaga ggatatccgg
    27781 ctgtacgttc tcatgctcgc aggcgaacat cttgcccgtg cgccccatgc ccgtttgtac
    27841 ttcatcgagg atcatcagtg cgccgaactc atcgcatagc ttacgtacgg cggtgagata
    27901 gcccggcggc ggcagaatta cgccaccttc accctgaatc ggttcgagga tcaccgcagc
    27961 cacatcatca ccggtttttt tgcactcgtt aagagccgtg cgcatggctt cgatattgcc
    28021 aaacggcaca tgacggaagc ccggcagtaa cggcataaac ggtttgcgga aggtcgattt
    28081 cgccgtggcc gacagcgcgc caagtgattt accgtggaac gcgccgctgg tggcaataaa
    28141 agtaaacttg ccgcgcggtg actggtaagc tttcgccagc ttcagcgctg cttcaacgga
    28201 ctcggtgccg ctattacaga agaagctgta tttcagttta ccgggcgtta gcgcagcaag
    28261 ggttttcgcc aacatcgccc gtaacggatc gagcagctcc tggctgtgca gcggttgttt
    28321 cgcaagttga ttctgtacgg cggaaaccac aactggatta cggtgcccca cgttgaaaat
    28381 tccaaaacct cccaggcagt cgataaactc ctgtccctgg gtgtcgacaa gcgtatttaa
    28441 acctcccgct tgccactcta cggctccgta atccccgccg gcggtaacag atttgcgata
    28501 ctctaaaaac cccggattga catgctcttt gaagtattca atcacctctc ggttaagtgc
    28561 tttcatctcc tcatgatcca gcgttcgctt ctcaatgaga ttcagggcgt gggcgctgca
    28621 cgctaaagcc gatgcgctcg aaggtaacct gttcaaaata tgctccggag gctcgcgtat
    28681 cacatgatac cgatttaagt attgcaggga ttgcgccact ccggctgcga tcgcgaaaat
    28741 cgggataaac acaaatgtgg ttatcatttg cgcaatattt aatgctattt gttaacatct
    28801 gttgttattt cgcacggttt ttgtcgggta tggatttgat gattgctgcg caaatgcacg
    28861 ttttcagggc aataattgcc tgatttttgg gcaagataat taacgttttc gtatagtaac
    28921 agagtgaaag tattttaaat caaagaatta gatttgtgct ttaatcgcaa attgcgatct
    28981 aaatcaaatt aatcggttaa agataaccgc agcggggccg acataaactc tgacaagaag
    29041 ttaacaacca tataacctgc acaggacgcg aacatgtctt ctcatccgta tgtcacccag
    29101 caaaataccc cgctggcggg cgataccact ctgatgtcca ctaccgatct gcaaagctat
    29161 atcactcatg ctaatgacac ttttgtgcag gtgagcggct ttaccttgca agagttacaa
    29221 gggcagccgc acaacatggt gcgtcacccg gatatgccaa aagcggcgtt tgcggatatg
    29281 tggttcaccc tgaaaaaagg ggagccctgg agcggcatcg tgaaaaatcg ccgcaaaaat
    29341 ggtgaccatt attgggtgcg ggccaatgcg gtaccgatgg tgcgcgaggg aaaaatcagt
    29401 ggctatatgt cgattcgtac ccgggcgacg gatgaagaga tcgcggcggt ggagccgctg
    29461 tacaaagcgc tgaacgccgg acgtaccggt aagcgtattc ataaaggcct ggtggtgcgt
    29521 aaaggctggc tgggtaaact gccttcatta ccgcttcgct ggcgggcgcg tggagtgatg
    29581 accctgatgt ttatcttgct ggcggccatg ctttggtttg ttgctgcccc ggtggtgacg
    29641 tatatcctct gtgcgttagt gatgttgttg gcaagcgcct gttttgaatg gcagattgtc
    29701 cgcccgatag aaaatgtcgc ccgtcaggca ctgaaggtgg cgaccggaga gcgtaatagt
    29761 gttgagcatc tgaatcgcag cgatgagctg gggctgacat tacgcgcggt tgggcagctt
    29821 ggcctgatgt gccgttggct aattaacgat gtctcaagcc aggtgtccag tgtcagaaat
    29881 ggcagtgaga cgctggcgaa aggcaccgat gaactgaacg aacataccca gcagacagtt
    29941 gataacgttc agcaaacggt ggcgaccatg aaccaaatgg cggcgtcggt gaaacagaac
    30001 tctgccacgg cgtcggctgc cgataaactt tctatcaccg ccagtaatgc ggcagtgcag
    30061 ggcggggagg cgatgactac ggtgatcaag acaatggacg atattgccga cagtacccag
    30121 cgcattggca ccattacttc gctgattaac gatattgcgt ttcagaccaa tattctggcc
    30181 ctgaatgcgg cggtggaagc ggcgcgtgcc ggcgaacagg gcaaaggttt tgcagtggtg
    30241 gcgggggaag tgcgtcattt agccagccgc agcgctaatg ctgccaacga tattcgcaag
    30301 ctgattgatg ccagtgctga taaggtgcaa tccggttcgc agcaggtaca tgccgccgga
    30361 cggacgatgg aagatattgt ggcacaggtg aaaaacgtca cccagttgat cgcccagatt
    30421 agccattcaa cgctggaaca ggccgatggg ctttccagcc tgacccgtgc agtggatgag
    30481 cttaacctga tcacccagaa aaatgccgag ctggtggaag agagtgcgca ggtgtcggcg
    30541 atggtgaaac accgcgccag ccgactggaa gacgcggtga cggtgctgca ttaatcggta
    30601 cgccggatcc ggctgttgga gcacaatgcc tgatgcgatg ctggcgcatc ttatcaggcc
    30661 tacggggtgt gtagcgggtg taggccggat aaggcgcgtc aagcgtcgca tccggcaatt
    30721 gcaccgcgcc actggcggat gcggcgatta atccagttgc gtaatatcaa aagcggcgcg
    30781 gtcgatcacc gcaatgatct tttttatttg tgcatcagtg atatcgctct ggttgacgcg
    30841 taaatccagc accgctttaa aattatccag cgcccgcttc atttgcgggt tctggcgcag
    30901 cgccgcgcca acgcaacgcg ctttgatgcg ttcttcaatc atctccacct gttcgcggtt
    30961 ttcttccagc cactgcgcgc cttgttcggt cagtgcaatc tgcttcttac caccttcctc
    31021 ttcgcggatg gtaatcagcg actgctcctg cagaaaatcc agcgtcgggt agatgacgcc
    31081 cgggcttggg gtgtaattcc cctgggttag attctcaatc gctttaatca attcgtaacc
    31141 gtggctgtca tcgcgcgaga gaatatccag aatcaccaga cgcaattcac cgtgaccaaa
    31201 gaaacgttgc cgacgaccgc cgccgcgacc gtggcgacca ccgcagcatt gaccgtgttc
    31261 atgctggtga ccgtgtccgc agtgctcgtg ttctgacttc tcacctttgc agcagccctc
    31321 atggcgtggc tggccttcat gtttacaaca cccttcgtga tgatggctca tatcagcctc
    31381 cttttgcttg attagatata tctaatttat atctgatata agtattactg caagtacaaa
    31441 aatgattttt aaacgcaggt ttactcaaca tgtaggccgg ataaggcgtt cacgccgcat
    31501 ccggcattga gcaatgatgc ttgataagag gttgcgcgtc ttatcagact acagatatat
    31561 cagttctaca aatcgcttgc atttatcatg attaacaatc attatcattt gcgagtttta
    31621 tttagatata tctaattacc atcacgaagg cgataacaat gaataactcc ccccgctacc
    31681 cgcagcgcgt tcgcaatgat ctgcgcttcc gtgaactgac tgtgttacgc gttgagcgca
    31741 tcagcgccgg ttttcagcgc attgtcctcg gcggcgaggc gctggacggt tttacatcgc
    31801 gtggctttga cgatcacagc aaactcttct ttcctcaacc tgacgctcac tttgtgccgc
    31861 caacggtaac ggaagagggc atcgtctggc cggaaggacc acgcccaccg tcgcgtgact
    31921 atacgccgct gtatgacgaa ctacgccatg aactggtgat tgatttcttt attcacgacg
    31981 gtggggtcgc cagcggctgg gcgatgcagg cgcaaccggg cgataaactt acggtggcag
    32041 gtccgcgcgg ttcgctggtg gtgccggaag attacgcgta tcagctgtat gtctgcgatg
    32101 aatccggaat gcccgcattg cgccgccgcc tggaaatgtt gagcaaactt gccgttaaac
    32161 cgcaagttag tgcgctggtt agcgtgcggg ataacgcctg tcaggattat ctcgcgcacc
    32221 ttgatggttt taatatcgaa tggctggcac atgatgagca ggcggtagat gcgcgtctgg
    32281 cgcagatgca aatccctgcc gatgattact tcatctggat aaccggcgaa ggtaaagtcg
    32341 ttaagaattt aagccgccgc tttgaagcgg aacagtatga cccacagcgg gtacgtgcag
    32401 cggcttactg gcacgcaaag taactgatat ataaaaagaa aaaggctgac gatttctcgt
    32461 cagcctttgc tatatctggt ggcccctgct ggacttgaac cagcgaccaa gcgattatga
    32521 gtcgcctgct ctaaccactg agctaagggg ccatggtagc ggattataaa gtaactccgt
    32581 gtcgcaatcc agccattacc gcgcgcctgc tgtttttata aacaatgtat tttcaatccg
    32641 ttatactttt cctggtgatg ttagaaagga gtaaatatgg ttgaggatat tttggctcca
    32701 gggttacggg tcgtgttttg cggtatcaac cctgggcttt catccgccgg gactggtttt
    32761 ccctttgctc atccggcaaa tcgcttctgg aaggtgatat atcaggccgg gtttaccgac
    32821 cgtcagttga agccgcagga ggcacagcat ctgctggatt atcgttgtgg cgtcaccaaa
    32881 ctggtagacc gtccaacggt gcaagccaat gaagtttcaa agcaggagct acacgcaggc
    32941 gggcgtaagc tgattgaaaa aattgaagat tatcagccgc aggcgttggc gattctgggc
    33001 aaacaagcat atgaacaggg attcagccag cgcggtgcac agtgggggaa acaaacgctc
    33061 accattggtt cgacgcagat ttgggtgctg ccaaatccca gcggtttaag tcgcgtttca
    33121 ctggagaaac tggttgaagc gtatcgcgag ctggaccagg cgctggtagt gcgtgggcga
    33181 taaaaaacgc caccgaaaag gtggcgtttg tgcagagggc ctagtgccgg gtgcggcgta
    33241 acgcctgatc cggcctaccg attaatcgtc caggaagcta cgcagcactt cagaacggct
    33301 cgggtgacgc agtttgcgca gcgccttcgc ttcgatctga cggatacgtt cgcgggtaac
    33361 gtcgaactgt ttacccactt cttccagcgt gtggtcggtg ttcatatcga taccgaaacg
    33421 catacgcaga acttttgctt cacgcgcggt caggccagcc agcacgtcgt gcgttgccgc
    33481 acgcaggctt tcggtggtcg cagaatccag cggcagctcg agggtggtat cctcgatgaa
    33541 atcccccaga tgcgaatctt catcatcacc gatcggcgtt tccatggaga ttggctcttt
    33601 ggcgatcttc agcactttgc ggatcttgtc ttccggcatc agcatacgtt cagccagttc
    33661 ttccggcgtc ggttcacggc ccatctcttg cagcatctgg cgagaaatac ggttgagctt
    33721 gttgatggtc tcaatcatat gcaccggaat acggatggtg cgcgcctgat ccgcgataga
    33781 gcgggtgatc gcctgacgga tccaccaggt tgcgtaggtg gagaacttgt aaccacggcg
    33841 gtattcgaat ttatcaaccg ctttcatcag accgatgttg ccttcctgaa tcaggtcaag
    33901 gaactgcaag ccacggttgg tgtatttctt agcgatagaa ataaccagac gtaagttcgc
    33961 ttcaaccatc tctttcttcg cacggcgggc tttcgcttca ccgatggaca tacgacggtt
    34021 gatatcttta acctgctcga tggtcaggcc ggtttcttct tcaatctgct gcagtttttg
    34081 cagggcgcga tgcacttctt cagagacatc gtgcagtttt tccgaccacg gcttgttcat
    34141 cgcaattgcc gcgttgaacc aggtatcgct ggtttcgttg ccggtaaaca gggtaatgaa
    34201 gtttttcttc ggcattttgc actgctcaac gcagagcttc atgatcagac gttcttgcgt
    34261 acgaacgcgg tccatcatga cgcgcatgct gttgaccagg tagtcaaact gcttcggcac
    34321 caggcggaac tgtttgaata cttcagacag tttcaggatc tcttcctgag cggtagcgtg
    34381 actgcgacct ttcgctttga tggtgtcacg cgttacaacg tactgagcgc gtagttccgc
    34441 aaatttttcg cgagccagtt ccgggtcgat gctgttgtca tcatcggcgc tgtcgtcatc
    34501 gccatcttct tcgtcttcat cttcgtcatc gtccagatct tcctgggaaa gctcagaacc
    34561 gacgtgagtg gcggtaggtg ccagatcttc ttctgcgttc gggtcaacaa agccggtgat
    34621 cagatcggac agacgcgctt cttctgcttc aacacgatcg tactgttcca gcagataggt
    34681 gatcgcttcc ggatattcag caacggagca ttgaacctgg ttgatcccgt cttcaatacg
    34741 cttagcgatg tcaatttcgc cttcgcgggt caacagttca acggtgccca tttcacgcat
    34801 gtacatgcgt accgggtcag tcgtgcgccc gatttcagat tccacgctgg aaagcacctg
    34861 cgcggcggct tcggcagcat cttcgtccgc ggtgttttca gccagcatca gatcatcggc
    34921 atccggtgct tcttccatca cctgaatgcc catgtcgttg atcatttgga tgatgtcttc
    34981 gatctgatct gaatcgacga tatcttccgg cagatggtca ttgacctcgg cataggtcag
    35041 atagccttgc tccttaccac gggtgacaag aagtttcagc tgtgactgcg ggttttgctc
    35101 cataagacgg tatccacact tatttcatga ggttggtgtt gggcgattga cccgatgtcc
    35161 ttcagccgtt aacgctgaaa tccaacggtt gtttaccgcc aacgataatt acgagggcgt
    35221 acttatatat ttgccgctgc ctctcagtgc ggctgtcggg ggcttcccga tcgctcttcg
    35281 gcacttaagc cgttaaatca ctttttcgcc agctcctggt ttaatgtcca gagctccagg
    35341 cgttcttcgt tgcttaaacc atgcgtgcgc tcacgagcga ttaactcttc ctggcgcagt
    35401 tcaagcagcg aatcaaacat atggttgagt gagtcggtga aggtttgctc agcaatattc
    35461 ttatctgcta tatcgtccca catcgacagt ttttcaaggg tggcagcatt atttgtacca
    35521 cgatagtgct ctaaaagttg cccggtggtc agacctggct gggagagaca agtgttgacc
    35581 agttctctga ataagccaag tccagggagc ttattttcat ccagattctc aagcggcggg
    35641 accaacgtcg ctaattctgg attttgcacc agcaacccta taagtatacg catggtcgtg
    35701 cgttttagct gcggaacagg gcgagaaacg ccgctctctg ccgcttttgg cattaatcgt
    35761 tcaagctggc tgtcatcaag tatgcctaat ttgttgccta attcctgacg aagatatatt
    35821 cgcagcgttt cgcccggcac ttgcgatatc aatggtagtg ccagcgtact caaacgtgcg
    35881 cgcccgtcag gggtactcag atcaacttgc ggcatcagac tgttaaacag aaatgcggag
    35941 agtggcatcg cctgctccat ccgcgcttca aacgcttctt taccttcttt tcgtaccagc
    36001 gtgtcagggt cttcgccatc aggcaaaaac ataaagcgta gctgacggcc gtctgtcatg
    36061 taaggcagcg ccgtttccag cgctcgccag gcggcatcgc ggcctgcacg gtcgccgtca
    36121 taacagcaaa tgacattgtt ggtcgcgcgg aacaacagtt gtatgtgatc ggcggtggtt
    36181 gacgtaccta acgacgcaac ggcgtaatta atgccgtatt gcgccagcgc caccacgtcc
    36241 atatagcctt cgaccacaag cagacgattg ggttcagcgt tatcctgctg cgcttcataa
    36301 agaccgtaaa gctggcggcc tttatggaaa atgtctgttt ccggcgagtt caggtatttg
    36361 ggggtatcgt tgcccagcac gcgcccgcca aaaccaatca cccgaccgcg tttatcgcga
    36421 atggggaaca tcacccgctc gcggaaacga tcgtaactgc gtccctgatc gttagtgacc
    36481 aacatccccg catcaatcaa tgactggcga ttttctggat tgccgccaaa ccgcttcagg
    36541 acgttgtccc agccgggggg cgcaaaacca atcgcaaagc gagcgataac ctcgtggctt
    36601 aatccgcgtt tttccagata ctggcgcgca gacgtggcaa caggttgttg taaagattgt
    36661 tggtaaaacg tattcagacc gtccatcaac tgataaagcg tttgcctctg atggcgctcg
    36721 atctggctgg ggccgctgcc tgcttcaaat ggcacttcaa gattgtgcat tgctgccagc
    36781 tcttcgaccg tttcgacgaa ctcgagcttg tcgtagttca tcaggaagtc gatcgcgttg
    36841 ccgtgcgcgc cacatccaaa gcagtggtaa aactgtttct caccgttaac ggtgaaggac
    36901 ggggttttct cgttgtggaa tggacaacac gcgtggaaat tcttgccctg ctttttcagc
    36961 ttcacacggg catcgatcag atcgacgatg tcagtgcgtg ccagcagatc attaatgaat
    37021 acgcgtggga ttcgtccagc cataggcccc gatttttagc aattcataaa cgaaaataag
    37081 ccgcgcattc ctttcggaag cacggcctta caactacaac tcggtctgat cggagagcaa
    37141 cgctctcggg gaattagtac agacgagtgc ggcgtgcgtt ttcgcgagcc agtttcttcg
    37201 cgtgacgttt cactgcagaa gctttagcgc gcttacgttc ggtagtcggt ttttcataga
    37261 actcacgacg acgaacttcc gccagaacac ctgctttttc gcaggaacgc ttgaagcgac
    37321 gcagagctac gtcgaacggc tcgttttcac gtactttaat taccggcatg tgcctctcac
    37381 ctttgattaa ttcggtttgc cgctggcatc aacgccagct tatttcaaaa tggtgcggaa
    37441 ttttactgca attgctgctg ctttgtaaag caccgcggcc ttttttgaaa gggactttta
    37501 taagggtgag gagtatacac gaggctttct ccaggggcga acaaagtttt acatcaaccc
    37561 gcattggtcc tacactgcgc ggtaataaag cgaggtaaaa caagtcatgc gtgtactggg
    37621 tattgaaact tcctgcgatg aaaccggcat cgccatttac gacgatgaaa aaggtttgtt
    37681 agccaaccaa ttgtatagtc aggtgaaatt gcacgctgac tacggcggcg tcgtgcctga
    37741 actggcctcc cgcgatcatg tgcgtaaaac cgtaccgttg atccaggcgg cgctaaagga
    37801 gtctggttta acggcaaaag acattgatgc tgtggcctat accgcaggcc ctggattagt
    37861 cggcgcgcta ctggttggcg cgaccgtggg gcgttctctg gcgtttgcct gggacgttcc
    37921 ggcgatccct gtacaccata tggaagggca tctgttagcg ccgatgctgg aagataaccc
    37981 gccggaattt ccgtttgttg cgctgcttgt ttccggcggt catacgcagt taatcagcgt
    38041 gactggcatt ggtcagtacg agctgctcgg cgagtctatc gatgatgccg ccggggaagc
    38101 gtttgataaa accgcgaagc tgctggggct ggattatcct ggcgggccgt tactgtcgaa
    38161 aatggcggct cagggtactg ccgggcgctt tgtcttcccg cgtccgatga ccgaccgtcc
    38221 ggggctggat ttcagcttct ccggcctgaa aaccttcgcg gcaaatacca ttcgtgacaa
    38281 cggcaccgac gaccagacgc gtgctgatat cgcccgcgcc tttgaagatg cggtggtcga
    38341 tacgctgatg attaagtgca agcgggcgct ggatcagacg ggctttaagc gactggtcat
    38401 ggcgggcggc gtgagtgcta accgtacgtt acgggcgaag ctggctgaaa tgatgaaaaa
    38461 acgccgcggc gaagtgttct acgcgcgtcc ggaattttgt actgataacg gcgcgatgat
    38521 cgcctatgcc ggaatggtgc ggtttaaagc aggcgcgacg gcggatctcg gcgttagcgt
    38581 gcgtccgcgc tggccgctgg cggagttacc ggctgcgtaa aactattggg tgccggagag
    38641 caatttccgg caccgtcctc acttaaagca acaccacggg catccacagt aagccggtaa
    38701 tcaccagcaa tacgaggaat atcagcccaa aaatcgcccc cagtcgccag taatccgccg
    38761 ttggcagata accactgccg tagtaaatcg gactggggcc ggtggcgtat ggtgtaagaa
    38821 tgctccccag accaattgcc gcaccaacca tcaggcagaa caccggcagc gggatttccg
    38881 gcatcgccag cgcagcggca atcatcatcg gtgcgagagc ggaggtatac gccgtggcgc
    38941 tggcgaaaaa gtagcgcagt agataaaaca ccacaatcaa cgccaccatc accatcgttg
    39001 gcgaataacc gcttaagctg cctgctaaca gtttgccaaa ccagctaata aaaccggtgt
    39061 tgttgagtcc ggtggcgagg gtgataagcg aggccagcca gaagaaaacg ttccacgccg
    39121 ctttattact gacaatgtcg tcccaactga taatgcgcag aagcagcatc agtgccacca
    39181 cgctgtaacc gaccatcgcg gcatcgatat aatcaccgcc gaaaatccac agcaccagcg
    39241 cgcctaccat cagccccagc atccgttttt cacgcgaaca gagcgggccc attgcctgca
    39301 gttccgtctc tgcccagcgc ggcacctgat cgccagactt cagtaccggc gggtacagca
    39361 cgtaagccag ccagggaacc agcagaacca gtaaaatgct gagcggcaac atcccgagga
    39421 accagtcgcc ccaactcagc gtggcgtgag atgcgctttt catcagtcca attaacagca
    39481 agtttggtgc catcgccgtc aagaaaatgg cgctggtcac gcagtcggca acaatcccca
    39541 tccacatgat gtacgagcca atgctgcgcg aactgctgtc gtttggttgt gattgataga
    39601 gcggtggcag gttacggatg atgggataga taatccccgc accacgcgcc gagttggacg
    39661 gtgttacagg tgcgaggatc agctcggaga acatcaccgc atagccgaga aacagcgtgc
    39721 gatgtcccat ctttttcacc agaatcagcg caatgcggcg tccaagcccg gttttttcat
    39781 agcctgtgcc aaacataaag gcggcgaaaa tcagccagat aaccgaatta gaaaaaccgg
    39841 aaacggccca cgagagggat tttgcagtga atttaaagcc tggctgagcg agctgctccg
    39901 ggctgaacag cagccaggga gagagtatgg cgatgatgga tatacccacc atcgccacca
    39961 cggcacccgg cacgggttcg aggatcagtc caacgatcac gccagtaaaa acggcaaagt
    40021 agagccaggt atggttctcc agccctgcgg gaaccggaag tagagcaata atggcgatga
    40081 ccgccagcgg cgcaagatat cgccaccatt cagtggaagg tttcatcgtg ttatgcctgc
    40141 gaaaaaggag aggggtgagc ctctccggga gggttatttg atgtaatgga cgtgctcgca
    40201 gatctcttcc acgatgggat cgcggcgttc ggcgaacagc tttttgtttt cggctatcag
    40261 gttgttgccg tgggtatcaa tagagacaat cagcgggccg aactctttga cccggcagac
    40321 ccacagtgac tccggcattc cgagctctgt ccagtgcact tcttcaatct cttccacctg
    40381 ggttgccgcc agcaccgcgc agcctgccgg gaaaatcaca tgtagcgcct tgaatttctg
    40441 gcagccttct tcggtcagcg gccccatacc acctttgcca accaccagtt tcacgccggt
    40501 ctgctcaata aattcacgtt caaaactttc catacgcata ctggttgtcg ggccgacgga
    40561 gaccatctcc catttgtcgc cgtttttgcg cacgatgggg ccagcgtgga aaatcgcttt
    40621 gccgttgaga tcgtaaggga tcggacgttt cagttcgatc aaacggcggt gacaaacgtc
    40681 gcggcaggtc accagcgtac cggtcaggta gatcacatcg ccgacgcgaa tatcttgcag
    40741 atcttcagct ttgatcgggg ttgtcaggat ctttttcata acgcgctccg ggtgtgagac
    40801 agattttcaa aggtgagatc ggcatgaacc agcagcgtgc cgcgacgatg cgcccagcag
    40861 ccggtagaga cagcaacgcc gatggttgac ggatggcggg cggcagattc gatatgtacg
    40921 cccatcactg aactgttgcc ggtcagccct tgtggaccaa tccccagacg gttgagtcct
    40981 tcttccaggc gtagctccag ttctgccgct tttggattgg gatggcggct gccaatcggg
    41041 cgcaaaatgg ctttacgcga gagtacggcg gcggtttcca ccgaggtggc gatgcccacg
    41101 cccaccagta ccggtggaca ggcgtttacg gcgagggtgg agatattttc gaagacgaat
    41161 ttcaccacgc cttcgtagcc ttctgacggc attaacactt tcgagcggcc aggtagcgtg
    41221 cagccgccgc ctgccatgta aacttcgatt tccgcatcgt cattgtcggg gatgatgtcc
    41281 caggtgaccc acggtacgcc gctaccggta tttttgccgg tgtttacttc gtcaaaaatt
    41341 tctaccgcat tgtgacgtag tggcgctttg acggttgcct cttccacggc ttgtttgagt
    41401 atgctttgca gctcgccaag cagtgggaag cgggaaccga ctttaacgaa gaacataatc
    41461 tccccggtgt cctgacaggc aggacgattc aggtcaatcg ctttttgcat gttgtcgaac
    41521 atcgtatggt agataatttt ccccatcgac gacgtttcgg catcctttag ctgttttagt
    41581 ttatccacca cgtcatcagg cattcgggta gaaatcatgg cggtaaagtt agcgacaatc
    41641 tctgtcaact tattcactgc ctgttgctta ttactttcgc tcatcatggc aactccagtt
    41701 tttgtatttg tctggaaaaa ttcaccgcca caaactacca ctaaaaatgt ggttttatat
    41761 tttttgccac cgtgaatatg taattaacag ttcgtgaatc aacgcgaaaa taagaatgaa
    41821 tattgcgcca gagcagggaa tggcaatgtt ttgcgaaata cactgcgctt atcacaactg
    41881 cggataatta acgatgctga atagctggcc tttagccaaa gacttgcagg tactggtaga
    41941 aatcgtccac agcgggagtt ttagcgccgc agcggcgacg cttgggcaga cgcctgcatt
    42001 tgtcaccaag cggatccaaa tccttgaaaa tacgctggca acaacgctgc tgaaccgctc
    42061 cgctcgcggc gtggcgctga ctgaaagtgg ccaacgttgt tacgaacatg cgctggaaat
    42121 cctcacccaa tatcagcgac tggtcgatga cgtcacgcaa atcaaaacgc gcccggaggg
    42181 gatgattcgt attggctgta gttttggttt tgggcgcagc catattgcgc cagccattac
    42241 cgaacttatg cgcaattatc ctgagttaca ggtgcatttt gaattgttcg atcggcaaat
    42301 tgatttagtg caggataata ttgatctgga tattcgtatt aatgacgaaa ttcctgatta
    42361 ttatattgcg catctgttaa cgaaaaataa aagaatatta tgcgcagcac ctgagtatct
    42421 gcaaaaatat ccgcagccac aatcattaca ggaattaagt cgtcatgact gtctggtgac
    42481 caaagagcgc gatatgaccc acggaatatg ggagttgggg aacggtcagg agaaaaaatc
    42541 ggtgaaggtt agcgggcatc tttcctccaa tagcggcgag atagtgttgc aatgggcgct
    42601 ggagggcaaa gggattatgc tgcgttccga gtgggatgtg ttgccgtttc tggagagtgg
    42661 caaactggtg caagtattgc cggagtatgc acaaagcgcc aatatctggg ctgtttaccg
    42721 ggagccgctc tatcgcagca tgaaattacg tgtctgcgtg gaatttctgg cggcatggtg
    42781 ccagcaacgg ctgggcaagc ccgatgaagg ctatcaggtc atgtagatcc accagaaatc
    42841 actcgggatc cttttcgcgc tttcttttga atttcgtcca gatttttgtc tcctgacgac
    42901 gccacagacg ctggatgttg tcatgatgac gcagcaggat caggcaagag agcatcgaaa
    42961 ccgggaaggt gaattgtggc ttaaaccacc agacataaaa cggagcaatc agtgcactga
    43021 caatcgctcc cagcgacgag tatccgctca atagcacggt cagtaaccag gttcccgcca
    43081 ttactccggt gagatcccag ccaatgggtg cgatggcacc aaaagcggta gcaacgcctt
    43141 ttcctccttt aaatccgaag aaaacgggcc agatgtgtcc aagacaggcg gcgatggcaa
    43201 ttaagcctag ccagaagggg ctgacaccta attcatacgc gccccagacg ggcaacattc
    43261 ctttcagaac gtcgaaaatc agtactgcta cggctgctcc cttgccaccg atacgtaaca
    43321 cattggttgc gcctggattg ccggagccgc tggttcgcgg atcgggcagc ccacacaagc
    43381 ggcaaaccag aatggcactg gaaatggagc cgcagaggta cgcgatgagg atcattccag
    43441 gcgcgattgc actcataagc tgttccgttt tgaaaattcg tgttcaacga ttaatctgtg
    43501 gataatacgc acatttcgcc ggaagtggta tccggttagc caaaaagcag gcaggacgtg
    43561 atggatattg tatttataga gcaactttcg gtaatcacca ctattggtgt ttacgactgg
    43621 gaacagacca tcgaacagaa gttagtgttc gatatcgaaa tggcatggga taaccgtaaa
    43681 gcggcgaaaa gtgatgatgt ggcggattgc ctcagttacg ctgacattgc agaaacggtg
    43741 gtcagccacg tcgagggggc gcgttttgcg ctggtggaac gcgtggctga agaggtggcg
    43801 gagctgctgt tagcacgctt caactcgccg tgggtgcgta tcaaactcag caagccaggc
    43861 gcagtggcgc gggcggcgaa tgttggcgta atcattgagc gtggcaataa tctgaaagaa
    43921 aataattaat tttacagctg ttaaaccaaa cggttataac ctggtcatac gcagtagttc
    43981 ggacaagcgg tacattttaa taatttaggg gtttattgat gagcgatatg cactcgctgc
    44041 tgatagcggc aatattgggt gtggtcgaag gattgacaga atttctgccg gtatccagca
    44101 cgggccatat gattattgtc ggtcacttgt tggggtttga gggcgacacg gcgaaaacct
    44161 ttgaagttgt gatccagtta ggatcaattc tggcggtagt agtgatgttc tggcggcgtc
    44221 tgtttggcct gattggcatc cactttggcc gcccgttgca gcacgaaggt gaaagcaaag
    44281 gtcgtttaac gctgatccac attttgctgg ggatgattcc ggcggtggta ttggggctgt
    44341 tgttccacga cacgattaag tcattgttta acccgataaa tgtgatgtat gcgctggtcg
    44401 ttggcggttt gttgctgatt gccgccgaat gcctgaagcc gaaagagccg cgtgcgccgg
    44461 gtcttgatga tatgacctat cgtcaggcat ttatgattgg ctgtttccag tgtctggcgc
    44521 tgtggccggg tttctcccgt tccggggcga ccatttcagg tgggatgctg atgggggtga
    44581 gccgttacgc tgcttccgag ttttcgttcc tgctggcggt gccgatgatg atgggcgcaa
    44641 cggcgctcga tctctacaaa agctggggct tcctgacaag cggcgatatc ccgatgtttg
    44701 ccgttgggtt tatcaccgct tttgtggtgg cgctgatagc gattaaaacc ttcctgcaat
    44761 tgattaagcg catttcgttt atcccgttcg ccatttatcg ctttattgtg gcggctgcgg
    44821 tgtatgtcgt gttcttttaa ttgaggaata gtgtcggatg cttggcgttg tcaacgattg
    44881 cgtaggccga ataaggcgtt cacgccgcat ccggcaatca acgcctgatg cgacgcttgc
    44941 gcgtcttatc aggcctacaa ctgtcgttga catgtagccg gataaggcgt ttacgccgca
    45001 tccggcaaaa tgcccaatac tcattcaggc tttgggcaac gttgttcctt ccagctggct
    45061 accgccgcaa tccgccgtcg ggtcagctcc tcgcgaatct ccacaccttt aaatcccgct
    45121 tcaacgacgg cttttgtcgg cactgactgc gccacttccc aggcttcgcg caaccagcgg
    45181 ccttgcgggt agtccgctga ttcaaaaccg gttctgccgc gcacgtcagc ctcgctggtc
    45241 agcgccagtt gctcgacacg ctgcggttta cgccaggcat cgatggaatc aaataatttg
    45301 acgatggttt ttgggttcag cattgggaag gtgtggatga gatcgtgaaa ctcagccacc
    45361 agtctggcta aatcgcgaat ttcatttggc acgcgcagac gctggcatag ttgttccact
    45421 aacttaacac ccgccgggcc atgaccatga tgacgcggcc agagttctgg cggcgtcagc
    45481 cctttaccga gatcgtggca taaagttgcg aaacggacat cgacctgcgg actcagcatc
    45541 gccgccattg agagcgtcat taaggtatga atacccgtat cgatttccgg atgccacttg
    45601 gcaggggccg gaacgccaaa cagtgcgtca atttccggga ataaaacgcg cagtgcgccg
    45661 caatcgcgca gtacctggaa gaatacctgt ggattgcggg tggtgagggc gctttccgtc
    45721 tctttccata cccgttcagg cgtcaggtgt tccagttcac ccgcatgggt catctcgcgc
    45781 atcaacgcca gagtttcatc ggcaatacga aaaccgaggt gggcataacg cgcagcaaaa
    45841 cgcgccacgc gcaatacgcg taacggatct tcgccaaaag cgggggaaac atggcgcaac
    45901 agacgatttt gcagatcgcc cagaccgttg tacgggtcga taatctcgcc gttatcgtcc
    45961 tgggccagcg cattaatggt cagatcgcga cgcttaagat catcttccag cgtgacatcc
    46021 ggtgcggcat agcaagtaaa accggtgtaa ccggaaccgg atttccgttc ggtacgtgcc
    46081 agcgcatact cttcatgcgt ttgcggatgc agaaacacag gaaaatcgcg gcctacctgc
    46141 tggtagcccg cgtcgagcat ctcctgtggc gtactgccga ccaccaccca atctctgtct
    46201 ttgaccggta gccctaacaa tgcatcccga acagcaccac cgaccagata aatcttcacg
    46261 ccatcatccc ttcgtcaaat acacaattcc taataataag acagtgcaac gaagaaggcg
    46321 atttagttca tccagcgatc tttgcgtttg cggcttggga tcaggtgcgg cagtaccaga
    46381 ccgagcagca agccaagccc cagcacgcca ccgccataca taaaccattg catgatgatg
    46441 gtgcgctgtt tgtcatccag ctgtacgctg gcggcatcga cctttttctg cgcgacaatc
    46501 agctcgtttt tcagtttctg attttcttct tttaacccgt tgatcacgct gtcgctctgc
    46561 gccacttttt gctgcatttc tgcggtgcgc tgattccagg tgttatcgat attggtgagt
    46621 ttatcggtca gggttttgac ctgattttcc agatctggca cacgggagcg caggcttggc
    46681 tcagtgctaa gttgtttcaa cgggatccag gcggtacggc cagagctgtc tttcacctgg
    46741 gcataattgg tgttggcgtc agtttgtaat aaggtcactt cctcgccggc gttaaccgtg
    46801 cccacgaggc gataatgatc tcccggaccg ctgcggaccc aggtattcag ttcgtcggaa
    46861 acatagcgcg tttcttcagc gtgtgagacg gcagtcgcgc taagtgcgag taaagttaat
    46921 ccgatcaggc gtaattttgg catcaggctg tcgttattgt catggaaagt ggaacgatag
    46981 tagtggcatc agtgtcgcta cgcaaagcat tcgacatcaa tcggaatcct ctgtgtcagg
    47041 tcttgccact gcttcaaact tttacgcccg tcaaattgcc cgttgcatgg caatttggcg
    47101 caaaatacta tctactgaca aaaaagatcg cattaagttc gccgttcatc gtcacttatg
    47161 tgacataacc gataagtaaa ggccatggct caggaaatcg aattaaagtt tattgttaat
    47221 cacagtgccg ttgaggcgtt gcgtgaccat ctcaatacgc tgggcggcga gcaccatgac
    47281 cccgtgcagt tgctgaatat ttactacgaa acgccggata actggctgcg tgggcacgat
    47341 atgggcttac gtattcgtgg cgaaaacggt cgctatgaga tgaccatgaa agttgcagga
    47401 agagtgacag gcggcttaca tcagcgcccg gaatataacg tggcgttgag cgaaccgacg
    47461 ctcgacctgg cgcagttacc gacggaagtc tggccgaacg gcgaattgcc cgccgatctc
    47521 gcctcccgcg tgcagccgct gttcagcacc gatttttatc gcgaaaaatg gctggtggcg
    47581 gtcgatggta gccaaattga aatcgccctc gaccaggggg aagtgaaagc gggtgaattt
    47641 gctgaaccta tctgtgagct ggaactggaa ctgcttagcg gcgacacgcg cgcggtgctg
    47701 aaactggcga accaactggt atcgcaaacc ggattacgcc agggcagcct gagcaaagcg
    47761 gcgcgtggct atcatctggc gcagggcaat ccggcgcgtg aaatcaaacc gaccaccatt
    47821 ttgcatgttg cggcaaaagc cgatgtggaa caggggctgg aagcggcgct cgagctggcg
    47881 ttagcgcaat ggcagtatca tgaagaactg tgggtacgcg gcaacgatgc ggcgaaagaa
    47941 caggtgctgg cagccattag cctggtccgt catacgctga tgctgttcgg tggtattgtg
    48001 ccgcgtaaag cgagcactca cttacgtgat ctgctgactc aatgcgaggc gaccattgct
    48061 tctgcggtgt ctgccgtgac ggcggtctac tctaccgaaa cggcaatggc gaagctggcg
    48121 ttgaccgaat ggttggtaag caaagcatgg cagccatttt tagatgccaa agcgcagggc
    48181 aaaatcagcg actccttcaa acgctttgcc gatatccatc tttcccgcca tgccgctgaa
    48241 ctgaaaagcg ttttctgcca gccgttaggc gatcgctacc gtgaccagtt gccacgcctg
    48301 acgcgtgata ttgactcaat actgttgctg gcgggttact atgatcctgt cgtcgcgcaa
    48361 gcctggctgg agaactggca ggggctgcat cacgctattg cgaccgggca acgcatcgaa
    48421 attgaacatt tccgtaatga ggcaaacaat caggaaccgt tctggttgca cagcggaaaa
    48481 cgttaatcag gcaaggataa aatcgcttat gaagccgctc tcttcaccgt tacagcagta
    48541 ctggcagacc gttgttgagc ggctgccaga gcctttagcc gaggaatcac ttagtgcaca
    48601 ggcgaagtca gtacttactt tcagtgattt tgtgcaggac agcataagtg cgcatccaga
    48661 gtggctgacg gaactggaaa gccaaccgcc gcaggccgac gaatggcagc attacgttgc
    48721 atggttgcag gaggcgctca gtaatgtgag tgacgaagcc gggttaatgc gcgagctgcg
    48781 gctgttccgt cggcgcatca tggtacgtat cgcctgggcg caaacgctgg cactggttac
    48841 tgaagagagc atattgcagc agctcagcta tctggcggag acgctgattg ttgcggcgcg
    48901 tgactggcta tatgacgcct gctgccgcga gtggggaacg ccgtgcaatg cgcagggcga
    48961 agcgcaaccg ctgctgattt taggcatggg taagctgggc ggcggggaac tgaatttctc
    49021 ctctgatatc gatctgattt ttgcctggcc ggaacatggt tgtacgcagg gtggacgccg
    49081 ggaactggat aacgcgcagt tttttacccg catggggcag cggctgatta aagtgctgga
    49141 tcaaccaacg caggatggct tcgtctatcg cgtggatatg cggctgcgtc cgtttggcga
    49201 aagtggcccg ctggtgctga gctttgccgc gttggaagat tattaccagg agcaggggcg
    49261 cgactgggag cgttacgcga tggtcaaggc gcggattatg ggcgatagcg aaggcgtcta
    49321 tgctaacgag ttgcgtgcga tgctgcgccc gtttgttttc cgtcgttaca tcgatttcag
    49381 cgtgattcag tcgctgcgca acatgaaagg gatgattgcc cgtgaagtgc gtcgacgtgg
    49441 tttgaccgac aatatcaaac tcggcgcagg cggcattcgc gaaattgaat ttatcgttca
    49501 ggtgttccag ctcattcgcg gcggacgcga accgtcgctg caatcgcgct ctttactgcc
    49561 aacgctcagc gccattgccg agctgcatct gctttctgaa aacgatgctg aacaattgcg
    49621 agtggcgtat ctgttcctgc ggcgtctgga aaacctgctg caaagcatta acgacgaaca
    49681 aacccagacg cttccttctg atgagcttaa tcgtgcgcgg ctggcgtggg cgatggactt
    49741 tgctgactgg ccgcaattga ccggggcgct gaccgcacat atgaccaatg tgcgccgggt
    49801 gtttaatgaa ttgattggcg acgacgaaag tgaaactcag gaagagtcgc tgtcggaaca
    49861 gtggcgtgag ctgtggcagg atgcgttgca ggaagatgac actacgccag tgctggcgca
    49921 tcttagcgag gatgatcgca aacaggtgct aacgctgatt gccgatttcc gcaaagagct
    49981 ggataagcgc accatcgggc cgcgaggacg tcaggtgctc gaccatctga tgccgcatct
    50041 gctaagtgat gtctgtgcgc gtgaagacgc tgccgttacg ctgtcgcgca ttaccgcctt
    50101 gctggtgggg attgttaccc gcaccaccta tttagaattg ctcagtgaat tccccgcggc
    50161 gcttaaacat ttgatttctc tgtgtgccgc gtcgccgatg attgccagcc agctggcgcg
    50221 ttatccatta ttgctggatg aattgctcga tccaaacacc ctttaccagc cgacggcgac
    50281 cgatgcctac cgcgatgagt tgcgccagta tttgctgcgc gtgccggaag atgacgaaga
    50341 gcaacagctt gaggcgctgc gtcagttcaa acaggcgcag ctgttacgca tcgccgcagc
    50401 ggatatcgcc ggtacgctac cggtgatgaa agtgagcgat cacttaacct ggctggcgga
    50461 agccatgata gatgccgtcg ttcagcaggc gtgggttcaa atggttgccc gctacggtaa
    50521 gccgaatcac ctgaacgaac gcgaagggcg tggttttgcg gtggtcggct acggcaagct
    50581 gggcggctgg gagttaggct acagttccga tcttgacctt atcttcctcc atgattgccc
    50641 aatggatgcg atgactgacg gtgagcggga aatcgacggg cggcagtttt atctgcgtct
    50701 ggcgcaacgc attatgcatc tgttcagtac gcgtacctct tccggcattt tgtatgaagt
    50761 ggatgctcga ctgcgtccgt ccggggcggc gggaatgctg gtgacatccg cagaagcatt
    50821 tgccgattat cagaaaaacg aggcctggac gtgggaacat caggcgctgg tgcgtgcgcg
    50881 tgtagtgtac ggcgatccgc agctcaccgc gcactttgac gcagtgcgtc gcgagattat
    50941 gacgctgccg cgtgaaggta aaactctgca aacggaagtg cgggaaatgc gcgagaaaat
    51001 gcgcgctcat ctcggcaata aacatcgcga tcgctttgat atcaaagctg atgaaggggg
    51061 aattaccgat atcgaattta ttacccaata tctggtgttg cgctacgctc atgaaaaacc
    51121 gaagttaacg cgctggtcag acaacgtgcg tattctggaa ctactggcgc aaaacgacat
    51181 tatggaagag caggaagcga tggcgctgac ccgtgcttac actacgcttc gcgatgaact
    51241 tcatcatctg gcattacagg aattgccggg ccatgtgtcg gaggattgct tcaccgcaga
    51301 gcgtgaactg gtgcgggcaa gctggcagaa gtggctggtg gaagaatgaa gtatggtatt
    51361 atcgcgcgca aattttgaat ctctcaggag acaggaatga aagtaacgct gccagagttt
    51421 gaacgtgcag gagtgatggt ggttggtgat gtgatgctgg atcgttactg gtacggcccc
    51481 accagtcgta tctcgccgga agcgccggtg cccgtggtta aagtgaatac catcgaagaa
    51541 cgtccgggcg gcgcggctaa cgtggcgatg aatatcgctt ctctcggtgc taatgcacgc
    51601 ctggtcgggt tgacgggcat tgacgatgca gcgcgcgcgc tgagtaaatc tctggccgac
    51661 gtcaacgtca aatgcgactt cgtttctgta ccgacgcatc cgaccattac caaattacgg
    51721 gtactttccc gcaaccaaca gctgatccgt ctggattttg aagaaggttt cgaaggtgtt
    51781 gatccgcagc cgctgcacga gcggattaat caggcgctga gttcgattgg cgcgctggtg
    51841 ctttctgact acgccaaagg tgcgctggca agcgtacagc agatgatcca actggcgcgt
    51901 aaagcgggtg ttccggtgct gattgatcca aaaggtaccg attttgagcg ctaccgcggc
    51961 gctacgctgt taacgccgaa tctctcggaa tttgaagctg ttgtcggtaa atgtaagacc
    52021 gaagaagaga ttgttgagcg cggcatgaaa ctgattgccg attacgaact ctcggctctg
    52081 ttagtgaccc gttccgaaca gggtatgtcg ctgctgcaac cgggtaaagc gccgctgcat
    52141 atgccaaccc aggcgcagga agtgtatgac gttaccggtg cgggcgacac ggtgattggc
    52201 gtcctggcgg caacgctggc agcgggtaat tcgctggaag aagcctgctt ctttgccaat
    52261 gcggcggctg gcgtggtggt cggcaaactg ggaacctcca cggtttcgcc gatcgagctg
    52321 gaaaatgcag tgcgtgggcg cgcagatacc ggctttggtg tgatgaccga agaggaactg
    52381 aagcttgctg tagcggcagc gcgtaaacgc ggcgaaaagg tggtaatgac caacggtgtc
    52441 tttgacatcc tgcacgccgg gcacgtctcc tatctggcaa atgctcgcaa actgggtgac
    52501 cgtctgattg tcgctgtgaa cagcgatgcc tccaccaaac ggctgaaagg ggattcccgc
    52561 cccgttaacc cgctcgaaca gcgtatgatt gtgctgggcg cactggaagc ggtcgactgg
    52621 gtggtgtcgt ttgaagaaga cacgccgcag cgcttgatcg ccgggatcct gccagattta
    52681 ctggtgaagg gcggcgac
//
LOCUS       DAMZET010000011        51650 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.011, whole genome shotgun sequence.
ACCESSION   DAMZET010000011 DAMZET010000000
VERSION     DAMZET010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.011"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1235
                     /locus_tag="POB34_000798"
     CDS             <1..1235
                     /locus_tag="POB34_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HDI5917791.1"
                     /translation="KVITTTKKAVPVKQTVTAPVIPSNTVLTASPVITEPATTVISIE
                     PANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEPFIDSFVRGFGYSMGVATTYA
                     LFSSIDWDDDDHDHHHRDGNGWQHNGDNINIDVNNFNRITGEHLTDKNMAWRHNPNYR
                     NGVPYHDQDMAKRFHQTDVNGGMSATQLPAPTRDSQRQAAASQFQQRTHAAPVITRDT
                     QRQAAAQRFNEAENYGSYDDFRDFSRRQPLTQQQKDAARQRYQSASPEQRQAVREKMQ
                     TNPQIQQRRDAARERIQSASPEQRQAVREKMQTNPQIQQRRDAARERIQSASPEQRQV
                     FKEKVQQRPLNQQQRDNARQRVQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQ
                     RSAVRERLSERGARRQER"
     gene            complement(1306..2730)
                     /gene="lpdA"
                     /locus_tag="POB34_000799"
     CDS             complement(1306..2730)
                     /gene="lpdA"
                     /locus_tag="POB34_000799"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDI5917792.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(2938..4830)
                     /gene="aceF"
                     /locus_tag="POB34_000800"
     CDS             complement(2938..4830)
                     /gene="aceF"
                     /locus_tag="POB34_000800"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDI5917793.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(4845..7508)
                     /gene="aceE"
                     /locus_tag="POB34_000801"
     CDS             complement(4845..7508)
                     /gene="aceE"
                     /locus_tag="POB34_000801"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HDI5917794.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(7669..8433)
                     /gene="pdhR"
                     /locus_tag="POB34_000802"
     CDS             complement(7669..8433)
                     /gene="pdhR"
                     /locus_tag="POB34_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HDI5917795.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            8974..10347
                     /gene="aroP"
                     /locus_tag="POB34_000803"
     CDS             8974..10347
                     /gene="aroP"
                     /locus_tag="POB34_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HDI5917796.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(10390..11244)
                     /gene="ampE"
                     /locus_tag="POB34_000804"
     CDS             complement(10390..11244)
                     /gene="ampE"
                     /locus_tag="POB34_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HDI5917797.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(11241..11792)
                     /gene="ampD"
                     /locus_tag="POB34_000805"
     CDS             complement(11241..11792)
                     /gene="ampD"
                     /locus_tag="POB34_000805"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HDI5917798.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            11880..12773
                     /gene="nadC"
                     /locus_tag="POB34_000806"
     CDS             11880..12773
                     /gene="nadC"
                     /locus_tag="POB34_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDI5917799.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            12976..13416
                     /gene="ppdD"
                     /locus_tag="POB34_000807"
     CDS             12976..13416
                     /gene="ppdD"
                     /locus_tag="POB34_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HDI5917800.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTHYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            13426..14811
                     /gene="gspE"
                     /locus_tag="POB34_000808"
     CDS             13426..14811
                     /gene="gspE"
                     /locus_tag="POB34_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HDI5917801.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLARTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVGQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMCTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            14801..16003
                     /gene="hofC"
                     /locus_tag="POB34_000809"
     CDS             14801..16003
                     /gene="hofC"
                     /locus_tag="POB34_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HDI5917802.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAILHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRENTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(16038..17081)
                     /gene="guaC"
                     /locus_tag="POB34_000810"
     CDS             complement(16038..17081)
                     /gene="guaC"
                     /locus_tag="POB34_000810"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDI5917803.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGYEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(17237..17281)
                     /locus_tag="POB34_000811"
     CDS             complement(17237..17281)
                     /locus_tag="POB34_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917804.1"
                     /translation="MIIKTLKMSARKRN"
     gene            17306..17926
                     /gene="coaE"
                     /locus_tag="POB34_000812"
     CDS             17306..17926
                     /gene="coaE"
                     /locus_tag="POB34_000812"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDI5917805.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            17926..18669
                     /gene="zapD"
                     /locus_tag="POB34_000813"
     CDS             17926..18669
                     /gene="zapD"
                     /locus_tag="POB34_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HDI5917806.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            18679..18876
                     /gene="yacG"
                     /locus_tag="POB34_000814"
     CDS             18679..18876
                     /gene="yacG"
                     /locus_tag="POB34_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HDI5917807.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(19092..19481)
                     /gene="mutT"
                     /locus_tag="POB34_000815"
     CDS             complement(19092..19481)
                     /gene="mutT"
                     /locus_tag="POB34_000815"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HDI5917808.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVERWEGKPWGKEGQPG
                     EWMALVDLNADDFPPANEPVIAKLKRL"
     gene            complement(19541..22246)
                     /gene="secA"
                     /locus_tag="POB34_000816"
     CDS             complement(19541..22246)
                     /gene="secA"
                     /locus_tag="POB34_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDI5917809.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(22308..22820)
                     /gene="secM"
                     /locus_tag="POB34_000817"
     CDS             complement(22308..22820)
                     /gene="secM"
                     /locus_tag="POB34_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HDI5917810.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPV
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(23051..23968)
                     /gene="lpxC"
                     /locus_tag="POB34_000818"
     CDS             complement(23051..23968)
                     /gene="lpxC"
                     /locus_tag="POB34_000818"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HDI5917811.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(24069..25220)
                     /gene="ftsZ"
                     /locus_tag="POB34_000819"
     CDS             complement(24069..25220)
                     /gene="ftsZ"
                     /locus_tag="POB34_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDI5917812.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(25281..26543)
                     /gene="ftsA"
                     /locus_tag="POB34_000820"
     CDS             complement(25281..26543)
                     /gene="ftsA"
                     /locus_tag="POB34_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDI5917813.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(26540..27370)
                     /gene="ftsQ"
                     /locus_tag="POB34_000821"
     CDS             complement(26540..27370)
                     /gene="ftsQ"
                     /locus_tag="POB34_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HDI5917814.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPERTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(27372..28292)
                     /gene="ddlB"
                     /locus_tag="POB34_000822"
     CDS             complement(27372..28292)
                     /gene="ddlB"
                     /locus_tag="POB34_000822"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDI5917815.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(28285..29760)
                     /gene="murC"
                     /locus_tag="POB34_000823"
     CDS             complement(28285..29760)
                     /gene="murC"
                     /locus_tag="POB34_000823"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDI5917816.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPARVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(29814..30881)
                     /gene="murG"
                     /locus_tag="POB34_000824"
     CDS             complement(29814..30881)
                     /gene="murG"
                     /locus_tag="POB34_000824"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDI5917817.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(30878..32122)
                     /gene="ftsW"
                     /locus_tag="POB34_000825"
     CDS             complement(30878..32122)
                     /gene="ftsW"
                     /locus_tag="POB34_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HDI5917818.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(32122..33438)
                     /gene="murD"
                     /locus_tag="POB34_000826"
     CDS             complement(32122..33438)
                     /gene="murD"
                     /locus_tag="POB34_000826"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDI5917819.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALHPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(33441..34523)
                     /gene="mraY"
                     /locus_tag="POB34_000827"
     CDS             complement(33441..34523)
                     /gene="mraY"
                     /locus_tag="POB34_000827"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDI5917820.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(34517..35875)
                     /gene="murF"
                     /locus_tag="POB34_000828"
     CDS             complement(34517..35875)
                     /gene="murF"
                     /locus_tag="POB34_000828"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDI5917821.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(35872..37359)
                     /gene="murE"
                     /locus_tag="POB34_000829"
     CDS             complement(35872..37359)
                     /gene="murE"
                     /locus_tag="POB34_000829"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDI5917822.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIVNADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(37346..39112)
                     /gene="ftsI"
                     /locus_tag="POB34_000830"
     CDS             complement(37346..39112)
                     /gene="ftsI"
                     /locus_tag="POB34_000830"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HDI5917823.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(39128..39493)
                     /gene="ftsL"
                     /locus_tag="POB34_000831"
     CDS             complement(39128..39493)
                     /gene="ftsL"
                     /locus_tag="POB34_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDI5917824.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(39490..40431)
                     /gene="rsmH"
                     /locus_tag="POB34_000832"
     CDS             complement(39490..40431)
                     /gene="rsmH"
                     /locus_tag="POB34_000832"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDI5917825.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(40433..40891)
                     /gene="mraZ"
                     /locus_tag="POB34_000833"
     CDS             complement(40433..40891)
                     /gene="mraZ"
                     /locus_tag="POB34_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDI5917826.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(41493..42497)
                     /gene="cra"
                     /locus_tag="POB34_000834"
     CDS             complement(41493..42497)
                     /gene="cra"
                     /locus_tag="POB34_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HDI5917827.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(42579..42665)
                     /locus_tag="POB34_000835"
     CDS             complement(42579..42665)
                     /locus_tag="POB34_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HDI5917828.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(42677..43168)
                     /gene="ilvN"
                     /locus_tag="POB34_000836"
     CDS             complement(42677..43168)
                     /gene="ilvN"
                     /locus_tag="POB34_000836"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDI5917829.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(43171..44895)
                     /gene="ilvI"
                     /locus_tag="POB34_000837"
     CDS             complement(43171..44895)
                     /gene="ilvI"
                     /locus_tag="POB34_000837"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HDI5917830.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVCNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            45014..45058
                     /locus_tag="POB34_000838"
     CDS             45014..45058
                     /locus_tag="POB34_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917831.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(45213..46157)
                     /gene="leuO"
                     /locus_tag="POB34_000839"
     CDS             complement(45213..46157)
                     /gene="leuO"
                     /locus_tag="POB34_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HDI5917832.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            46817..46903
                     /gene="leuL"
                     /locus_tag="POB34_000840"
     CDS             46817..46903
                     /gene="leuL"
                     /locus_tag="POB34_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HDI5917833.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            46996..48567
                     /gene="leuA"
                     /locus_tag="POB34_000841"
     CDS             46996..48567
                     /gene="leuA"
                     /locus_tag="POB34_000841"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDI5917834.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            48567..49658
                     /gene="leuB"
                     /locus_tag="POB34_000842"
     CDS             48567..49658
                     /gene="leuB"
                     /locus_tag="POB34_000842"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDI5917835.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            49661..51061
                     /gene="leuC"
                     /locus_tag="POB34_000843"
     CDS             49661..51061
                     /gene="leuC"
                     /locus_tag="POB34_000843"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDI5917836.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            51072..>51650
                     /gene="leuD"
                     /locus_tag="POB34_000844"
     CDS             51072..>51650
                     /gene="leuD"
                     /locus_tag="POB34_000844"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDI5917837.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYE"
BASE COUNT        12413 a        14280 c        12741 g        12216 t
ORIGIN      
        1 aaaaagttat taccacaacg aagaaagctg taccggtaaa acagacagtc acggcacccg
       61 tcataccatc caataccgtt ttaactgcca gccccgtcat tacagagcct gcaacaaccg
      121 tcatttccat tgagcccgcc aatcctgatg tggtctatat tcccaactac aacccaaccg
      181 tggtttacgg gaactgggcc aatactgcgt atccgccggt ttatctgcca ccaccagccg
      241 gagaaccgtt tattgacagc tttgtgcgcg gattcggcta tagcatgggt gttgctacca
      301 cgtacgcact attcagcagc atcgactggg atgacgacga tcatgaccat catcatcgtg
      361 acggtaatgg ctggcaacac aacggcgaca acatcaatat cgacgtcaac aatttcaacc
      421 gtatcaccgg tgagcatctt actgataaga atatggcatg gcggcacaat ccaaactacc
      481 gtaatggtgt gccctatcat gatcaggata tggcaaagcg gtttcatcaa accgatgtca
      541 acggcggaat gagcgccacg cagctacctg ctccaacacg cgacagccag cgtcaggcgg
      601 cagcaagtca gtttcagcaa cgaacacacg ccgcccccgt cattacacga gatacccaac
      661 gtcaggcagc ggcacagcgg tttaatgaag ctgaaaacta tgggagctat gacgacttcc
      721 gcgacttcag ccgtcgccaa ccactgaccc agcaacaaaa ggacgccgct cgtcagcgtt
      781 atcagtcggc ctcgcctgag cagcgccagg cagtccgcga gaaaatgcag actaacccac
      841 agatccagca gcgaagagac gcagcgcgtg agcgtattca gtccgcctcg cctgagcagc
      901 gccaggcagt ccgcgagaaa atgcagacta acccacagat ccagcagcga agagacgcag
      961 cgcgtgagcg tattcagtca gcctcgcctg agcagcgcca ggtgtttaag gaaaaagtac
     1021 agcagcgccc actgaaccaa cagcaacgtg ataacgcccg ccagcgtgtt caatcagcat
     1081 cacctgaaca acgtcaggtt tttcgggaaa aagttcagga gagccgccca caacgtctaa
     1141 acgacagtaa ccatactgtc aggctgaata acgagcaacg gtcagcagta cgcgaacgtc
     1201 tctctgagcg cggagcaagg cgacaggaaa ggtaaattgc agacgtaaaa aaagcggcgt
     1261 ggttagccgc ttttttaatt gccggatgtt ccggcaaacg aacaattact tcttcttcgc
     1321 tttcgggttc ggcaggtcgg taatgctacc ttcgaacact tctgccgcca ggcccacaga
     1381 ctcgtgcaga gtcgggtgcg cgtggatggt cagtgcgatg tcttcagcat cacaacccat
     1441 ttcgattgcc aggccgattt cacccagcag ctcgccgccg ttggtaccga caatcgcacc
     1501 accgatcaca cggtgagatt ctttgtcgaa aatcagcttg gtcataccgt ctgcgcagtc
     1561 ggaagcgata gcacgaccag aagcagccca cgggaaggtg gcggtttcat agctgatgcc
     1621 tttctctttc gcttctttct cagtcagacc tacccatgca acttctggtt cggtataggc
     1681 gatggacggg ataactttcg gatcgaagta gtgtttctta ccggcgataa cttcagcggc
     1741 aacgtgacct tcgtgaacac ctttgtgtgc cagcatcggc tgaccgacga tatcgccgat
     1801 agcaaagatg tgcggtacgt tggtacgcag ctgtttgtca acgcggatga aaccacggtc
     1861 gtccacttcc acgccagctt tgcctgcgtc gaggttttta ccgttcggca cacgaccaat
     1921 cgctaccagc acggcgtcgt aacgctgcgg ttcagcgggt gcttttttgc cttccatcgt
     1981 cacataaata ccgtcttctt tcgcttcaac ggcggtaact ttggtttcca gcatcaggtt
     2041 gaatttcttg ctgatacgct tggtgaagac tttaacgatg tctttgtcag ctgccgggat
     2101 aacctggtcg aacatttcaa ccacgtcaat ctgtgaaccc agcgcgtggt atacggtgcc
     2161 catttccaga ccgatgatac cgccacccat taccagcagg cgttctggta cttctttcag
     2221 ttccagcgcg tcagtggagt cccagatacg cggatcttca tgcggaataa acggcagttg
     2281 gatcgggcga gaacccgctg caatgatcgc gttgtcgaag ttgatcacgg ttttaccgtt
     2341 ctcaccttca acttccaggg tgttagcccc ggtaaattta cccagaccgt tgaccacttt
     2401 gactttgcgg cctttcgcca taccagccag accaccggtc agctgattga tcactttctc
     2461 tttccaggta cgaatcttgt caatatcggt tttcggttcg ccgaagacga taccgtgttc
     2521 agccagcgct ttggcttctt cgataacttt tgctacgtgc agcagtgctt tagaagggat
     2581 acagccgacg ttcaggcaaa caccgccaag ggtgttgtaa cgttctacga ttacggtttc
     2641 cagacctaaa tcagcgcaac ggaaggcagc ggagtaacct gcggggcctg ccccaagtac
     2701 cacgacctga gttttgattt cagtactcat catgacctct atatatttat ctccggcggg
     2761 tctgacgttt tttttatgac gcccattatc caccgggtcg ttctatccgt cggtatttta
     2821 caaaattgtt aacaattttt aaacaacaaa cggcaaccga tttgtctatt cgctaataac
     2881 ctcaatacat tcatgagatt accagaaaaa agccggccgt tgggccggct cttttactta
     2941 catcaccaga cggcgaatgt cagacagcgt gttgttaatg atggtaatga aacgggcacc
     3001 atcagcaccg tcgatcacgc ggtggtcgaa ggagagagaa atcggcagca tcagacgcgg
     3061 cacgaactct ttaccattcc acaccggctc catcgcggac ttggaaacgc cgaggatagc
     3121 cacttccggc gcgttcacaa tcggcgcgaa gtgggtagta cccaggccgc cgatgctgga
     3181 gatggtgaag caaccgccct gcatttcgcc cgcagtcagc ttaccgtcac gcgctttctt
     3241 agaaatagtc atcagctcgc gagacagctc gatgatgcct ttcttgttga cgtctttgaa
     3301 taccggaaca accagaccgt tcggggtatc caccgccaca ccgatgttga tgtatttctt
     3361 cagggtcaga cgctgaccgt cttccgacag cgaactattg aagcgaggca tctgctcaag
     3421 agctgcagca acggctttca tgatgaagac aaccggggtg atcttcacat ccagcttacg
     3481 tttcgccgct tcttcgttct gctgtttacg gaacgcttcc agctcggtga tatcggtttt
     3541 gtcgaagtga gtaacatgcg ggatcatcac ccagttacgg ctcaggttcg caccagaaat
     3601 tttctggata cggcccagtt ccacttcttc gatttcacca aacttgctga agtccacctt
     3661 cggccacggc agcatgcctg ggataccgcc gccagtcgcc gccggagctg cttctgcacg
     3721 tttgatagct tctttcacgt aagcctgaac gtcttcgcgc aggatacgac ctttacggcc
     3781 agtgcccttc actttcgcca ggttaacgcc aaactcgcgt gccagacggc ggatcagcgg
     3841 agtcgcgtga acgtaagcgt cgttttctgc aaattcagat ttgccttccg ctttcgcagc
     3901 cggtgctgct gccggagctt cagcttttgc tgccggagcc ggcgctgccg cttcctgttt
     3961 cgcaggagct gccgcaggcg ctgcgccttc aacttcgaag atcataatca gcgagccagt
     4021 tttcacttta tcgccaacgt tgactttcag ttccttcacg acgcctgcaa acggagccgg
     4081 aacttccata gaagctttgt cgccttctac ggtaatcagt gactgttcag cggcaacttt
     4141 gtcgcccact ttcaccatca cttcggtcac ttcaacttcg tcaccgccga tatccggaac
     4201 gttaacttct ttcacgccag ccgctggtgc aggggccgct gccggagccg cttcctgttt
     4261 agccgccgga gctgccgcgc ctgcttcacc cgcgacttcg aagaccataa tcagcgagcc
     4321 ggtagacact ttgtcaccca cgttcacttt gatctctttc acggtgccag caaacggagc
     4381 cggaacttcc atagacgctt tatcgccttc tacggtgatc agcgactgtt cagcttcaac
     4441 tttatcgcca actttcacca ggatttcggt cacttcaact tcgtcgctgc cgatatccgg
     4501 aacgttaacg tcttttgccg ctgcagccgc tggtgctgct gccggagctg cttctttctt
     4561 ctcttctgcc tgagcaggtg cagcgtctgc tgcaccgtcg gcggaatcga aaatcataat
     4621 cagtgcgccg gtctgggttt tatcgccaac agagactttg atctctttaa cgatacccgc
     4681 ctgcggagac ggaacttcca tagaggcttt gtcgccttct acggtgatca gcgactgttc
     4741 ggcttcaact ttgtcgccca ctttgaccag gatctcggtg atttcaactt catcagcccc
     4801 gatgtccggt actttgattt cgatagccat tattctttta cctcttacgc cagacgcggg
     4861 ttaactttat ctgcatcgat gttgaatttg gcgattgcgt cagcaaccac tttcttatcg
     4921 atttcgccac gtttagccag ttcgcccagc gccgctacaa ccacgtagga agcatcaact
     4981 tcgaagtggt gacgcaggtt ctcacggctg tcggaacgac cgaagccatc agtacccagt
     5041 acgcggtagt cgtcagccgg tacgtaagta cggacctgct cagcgaacag tttcatatag
     5101 tcggtagatg ccactgccgg agcgtcgttc atcacctgag cgatatacgg aacgcgcgga
     5161 gtttccagcg ggtgcagcat gttccagcgt tcacaatcct gaccatctcg cgccagttcg
     5221 gtgaaggagg tcacgctata aacgtcagaa cctacgccgt aatctttcgc caggatctct
     5281 gccgcttcac ggacgtgacg caggatggaa ccggagccca gcagctgaac tttacctttg
     5341 ctaccttcaa tggtttcgag tttgtagata cctttacgga taccttcctc agcaccttcc
     5401 ggcattgccg gcatgtggta gttttcgttc agcgtagtga tgtagtagta aacgttctct
     5461 tgtttttcac cgtacatacg ctccagaccg tcatgcataa tgacagcaac ttcgtaagcg
     5521 taagccgggt cgtaagagat acagttcggg atagtcagcg actgaatatg gctgtgacca
     5581 tcttcgtgct gcagaccttc accgttcagg gtggtacgac cggaagtacc gccgatcagg
     5641 aagccacgcg cttgctggtc gccagccgcc cagcacagat cgccgatacg ctggaagccg
     5701 aacatcgagt aatagatgta gaacggaatc atcggcagat tgttggtgct gtaagaggtc
     5761 gccgctgcca gccaggaaca acctgcgccc aactcgttga tcccttcctg cagaatctga
     5821 cctttctcgt cttctttata gtaagcaacc tgctcgcggt cctgcggggt gtactgctga
     5881 ccgttcgggc tgtaaatacc aatctgacgg aacagacctt ccataccgaa agtacgcgct
     5941 tcgtcggcga tgatcggtac cagacgatct ttgatcgact tgttcttcag cattacgttc
     6001 agggcacgaa cgaaagcgat agtggtagag atctctttgc tctgctcttc cagcagcgcg
     6061 ccgaagtctt gcaggctcgg cagctcaagc ttctcggtga agttcggctg acggcttggc
     6121 agataaccgt gcagtttctg acgctgtgcg tgcagatagg tatgctcttc agaaccttcc
     6181 gggaaggtga tgtacggcag tttttcgata tctgcatcag acaccggcac attgaaacgg
     6241 tcgcggatgt gacgcacgcc gtccatgttc attttcttaa cctggtgcgc gatgttttta
     6301 ccttcagccg cgtcgcccat gccgtaacct ttaatggtat gagcaaggat tactgtcgct
     6361 ttgcctttgg tttcctgcgc tttcttgaat gcagcgtaga ttttcttcgg atcgtgacca
     6421 ccacggttca gtgcccagat ctgctcgtca gtccagtctg caaccagtgc tgcggtttca
     6481 ggatatttac cgaagaagtg ttcacgaacg tacgcaccat ctttcgattt gaaggtctgg
     6541 tagtcgccgt caacggtttc gttcatcagc tggatcagtt taccgctggt atctttacgc
     6601 agcagttcat cccaacggct accccacatc actttgatca cgttccagcc agcaccttcg
     6661 aagatgcctt ccagttcgtt gatgatcttg ccgttaccgg tgaccgggcc gtcaagacgc
     6721 tgcaggttac agttgataac gaagaccagg ttatccagtt tttcacgggt agcgatggtg
     6781 atcgcacctt tggattccgg ttcgtccatt tcaccgtcgc cgaggaacgc gtaaacggtc
     6841 tgtttagagg tatctttcag gccacggtgt tccagatatt tcaggaattt agcctggtaa
     6901 atagcaccaa tcggccccag acccatagat acggtcggga actgccagaa ttccggcatc
     6961 agtttcgggt gcggatagga agagaggcca ttgccgtgaa cttcctgacg gaagttatcc
     7021 agctgctcct gagtcagacg accttccagg aaagcacgag cgtacacgcc cggggagatg
     7081 tggccctgga agtaaaccag gtcgccgcca tcctgctcgt tgcgtgcacg gaagaagtgg
     7141 ttaaagcaca catcataaat ggttgcggaa gactggaagg acgccatatg gccgcccagt
     7201 tcgaggtctt ttttcgacgc acgcagcacc gtcatgatgg cgttccagcg gatagctgaa
     7261 cgaatacggc gttccagttc cagattaccc ggatactccg gttgttcttc aacggggatg
     7321 gtgttgatgt agttgctgat acctgtgcct gcggctacgt ttacaccgcc tttgcgggct
     7381 tcagcaagca gttggtcgat cagatactga gcacgctcaa caccttcttc acggatgacc
     7441 gattcgatcg cctggagcca gtcgcgagtt tcgatcggat ccacgtcatt tgggaaacgt
     7501 tctgacatgg gttattcctt atctatctaa taacgttgag ttttctggaa cctgtcccat
     7561 tgaactctcg ccggaaagct caataagaca ggttctacgt ttagttgccg cgcttttata
     7621 tgcgcttgat ttacaacatc ttctggataa tttttaccag aaaaatcact aattctttcg
     7681 ttgctccaga cgacgcagag aacgctcacg gcggctctct tcacgacttc tgtcgagcaa
     7741 aatttcttcg ataaaggcca gatggcgatg cgatgcttcg cgcgcttctt ccggcttacc
     7801 ggccataatc gcttcaaata tgcgggtgcg gtgactactc accagcggca gcatctcgcg
     7861 acgcgaatag agcaattcga agttctggcg gacattctgg gccaacatcg gctccataca
     7921 ccttagcaga tgaagcagaa ccacattgtg ggccgcttcg gtgacggcaa tctgatactg
     7981 gagtacggcg tttgattccg cgtccagatc gccagactgc tgcgccagct ctatggcgtg
     8041 gtggagttca cggatgcgtt ccttgtcttc atcggtacta cgcagcgcgg cgtaataagc
     8101 ggcgatacct tccagggcgt gtcgtgtttc gagcaagtca tactgtgact caggatggtc
     8161 ggagagcagc tccaccagcg gatcgctgaa gctttgccat aggctgctct ggacaaaagt
     8221 gccgccaccc tggcgacgaa gcaacaagcc cttcgcttcg agacgttgaa tcgcctcacg
     8281 caaggaggga cgggagacgt caaactgttt tgccagttcg cgttccggtg ggagtttttc
     8341 gcccgggcgg agagtgcctt cgaggatcaa aaactccagt tgctgctcaa tcacatcgga
     8401 gagttttggt tggcggattt tgctgtaggc catgagttcc tgtcttaagc cacttgccga
     8461 agtcaattgg tcttaccaat ttcatgtctg tgacgctaaa gtaacaaagt attcacctta
     8521 tgtccataca ggttttgatt gaaatcatga aactgtgcac attttaacaa cttgacatat
     8581 ataacgtttc aaagttgtaa ctatgcacaa atgtagactt tacgtaggaa aggagttttt
     8641 taacgattat taaactataa aaatccgaat tgaaccgatt cacttaccaa ttttgtgatt
     8701 tttaattcaa ttaaaacgaa tttaaattca ttctacatat tgagaggggt tgaggctgag
     8761 ctttacaaac ggtttctttt taagcaactc atcttcaacc atgcataaag cgggtgcatt
     8821 cgctgccgca taccattatt cttgatctga cggaagtctt tttgtaacaa ttcaaacttc
     8881 tttgatgtaa acaaattaat acaacaaacg gaattgcaaa cttacacacg catcactgcg
     8941 tagatcaaaa aaacaaccac cgcacgaggt ttcatgatgg aaggtcaaca gcacggcgag
     9001 cagctaaagc gcggccttaa aaaccgccat attcagctta tcgcgctggg tggcgcgata
     9061 gggaccgggt tattcctggg tagcgcctcc gtaatacagt ccgcagggcc agggattatc
     9121 ctgggttacg ccattgctgg ttttatcgcc tttctgatca tgcgtcagct gggtgaaatg
     9181 gtggtcgaag aacctgtcgc aggctccttt agccactttg cttataaata ctggggcagt
     9241 tttgccggtt tcgcctctgg ctggaactac tgggtactgt acgttttagt tgccatggct
     9301 gagctgactg ccgtgggtaa atacattcag ttctggtatc cggaaatccc cacctgggtt
     9361 tctgccgccg tattctttgt ggtgattaac gccatcaacc tgaccaacgt taaagtgttt
     9421 ggcgagatgg agttctggtt tgccattatc aaagttatcg cggtggtagc gatgatcatc
     9481 ttcggcggct ggctgctatt cagtggcaac ggcggcccgc aggcgaccgt tagcaacctg
     9541 tgggatcagg gtggtttcct gccgcacggc ttcaccgggc tggtgatgat gatggcgatt
     9601 atcatgttct cgttcggtgg tctggaactg gtggggatca ccgcagcaga agctgataac
     9661 ccggagcaaa gtataccgaa agcaactaac caggttatct accgcatcct gattttctat
     9721 attggttcgt tagccgttct gctctcactg atgccgtgga cccgcgttac cgccgatacc
     9781 agtccgtttg tgctgatctt ccacgagtta ggcgatacct ttgtggcgaa tgcgctgaac
     9841 atcgtggtac tgactgcggc gctctccgtg tacaacagct gcgtatattg caacagccgt
     9901 atgctgtttg gtctggcaca acagggtaat gcgccaaaag cgctggcgtc tgtcgataaa
     9961 cgtggtgtac cagtaaatac cattctggtg tctgcactgg taacggcgtt gtgcgtactg
    10021 attaactacc ttgccccaga gtccgctttc ggactgttaa tggcgctggt ggtatctgca
    10081 ctggtaatca actgggcgat gattagcctg gcgcatatga aattccgtcg cgccaagcag
    10141 gaacaaggcg tggtaactcg cttccctgct ctgctttatc cgctgggtaa ctggatctgc
    10201 ctgctgttta tggcggcggt actggtgatt atgctgatga ccccaggaat ggcgatttcg
    10261 gtatacctga tcccggtatg gctgatcgtg ttaggtatcg gctatctgtt taaagagaaa
    10321 accgccaaag ccgtaaaagc gcattaatct ctctacgccc tcacccgtac agggtgaggg
    10381 caataatctt tacaccaacg ccccgtaaat cgtcagtagt gcaatcacca ccacgaccac
    10441 gaacgaggtt ttcttcgcca ttgaaaccgc tgccttcggc gtctccacct tatcgacatg
    10501 cggttcacgc gccagagaga actgcgccag acgcgttaac acctgatact gcgaagtatg
    10561 gaaatcaccc agcgaagcaa accaggccgg taacgctttc tcaccatgac cgatcaaggc
    10621 atataccaca cccgcaagac gaaccggcac ccaatccagt acatgaagca cggcatcaat
    10681 gccggactgt aaacgatgat gcggcgtctg atatcgcgcc agccagtatt gccatgcacg
    10741 caaaaacgca taccccatca gcgtaacggg tccccaggtt ccccccacaa tcagccagaa
    10801 cagcggtgca agataaaaac gaaagttaat ccacagcaat gcattttgca gctcacgcaa
    10861 atactcacgt tcgtcgcagc ctgccgggac gccgtgaatc atggtgagtt cgccagccat
    10921 cgtggcacgg gcatggctat cattacgtga agcagctgtc agataagcat gataatgaag
    10981 acgaacttta cctgcgccaa tacacagcaa accaatcagc agccacacca gtagcgtggg
    11041 aacgttgaac aatactccct gcaatgcgcg taacagtaaa aaagtcacgc ccatcgcaat
    11101 aatggtcatg cctaacgtgc gcccgagaga aaaatgtttc acccgccgaa agaacgcttc
    11161 aagacgatga tcaagctgcc agtgctcgcc caacttaaac aggcgctcga aaattaacac
    11221 cagtaaggtt gtaaatagcg tcatgttgtc tccttgctga ccagcacacg aaaccgtgcc
    11281 caatcaaatg caggaccggg atcggttttc cgatccggcg caatatcaca atggcccgtc
    11341 atgtttttag cgatatccgg atagcaatca atcagtgccc gcgtaaccgc cgcaagctgt
    11401 tgatactgcg catcggtata cgccagcgta tcggtgcctt caagctcaat cccaatagaa
    11461 aaatcattgc agcgttcgcg cccctgatac tgagagactc ccgcatgcca tgcacgttta
    11521 tcgaaaggaa catactggac tatttcacca tcacggcgaa tcaaacagtg agcggagacg
    11581 cgcaaatggg cgatctcagc aaagaaagga tgtgcctgcg gatcaatagt tccagtgaat
    11641 aatgcgtcga tccacggacc gccaaactcg cctggcggca ggctaatatt gtgcaccacc
    11701 agcagggtgg gtgtttcgtc atccgggcgg caatcgtaat gtggtgaggg aacgcggcgc
    11761 gcgccaacca gccacccctg ttctaacaac atgcaggatc tccttatatg tggtgctaat
    11821 acccggttca gagtagcatg tttctacctt atgattcgtt agctatctgg agttttaaca
    11881 tgccgcctcg ccgctataac cctgacaccc gacgtgacga gctgctggaa cgcattaatc
    11941 tcgatatccc cggcgcggtg gcccaggcgc tgcgggaaga tttaggcgga acagtcgatg
    12001 ccaacaatga tattacggca aaacttttac cggaaaattc tcgctctcat gccacggtga
    12061 tcacccgcga gaatggcgtc ttttgcggca aacgctgggt tgaagaggtg tttattcaac
    12121 tggcaggcga cgatgtcacc ataatctggc atgtggatga cggcgatgtc atcaatgcca
    12181 atcaatcctt gttcgaactt gaaggcccat cccgcgtgct gttaacgggc gaacgcactg
    12241 cgcttaattt tgtgcaaacc ctttcaggag ttgccagtaa ggtacgccac tatgtcgaat
    12301 tgctggaagg caccaacacg cagttgttgg atacgcgcaa aaccttaccc ggcctgcgtt
    12361 cagctctgaa atacgcggta ctttgcggcg gcggagcgaa tcaccgtctg gggctttctg
    12421 atgccttcct gatcaaagaa aaccatatta ttgcctccgg ctcagtgcgc caggcggtcg
    12481 aaaaagcgtc ctggctgcac ccggatgcgc cagtagaagt cgaagtagag aatctggaag
    12541 aacttgatga agccctgaaa gcaggagccg atatcatcat gctggataac ttcgaaacag
    12601 aacagatgcg cgaagccgtc aaacgcacca acggcaaggc gctactggaa gtgtctggca
    12661 acgtcactga caaaacactg cgtgaatttg ccgaaacggg cgtggacttt atctccgtcg
    12721 gtgcgctaac taaacacgta caagcactcg acctttcaat gcgttttcgc taatagttga
    12781 cagatttatc cgctccatcg cgggcggata atctcccctt ccccaacttt cttcgtaacg
    12841 cctcgcaaat ttcttgtctt tcgctgcaac gaaatagaaa acctcggaat ctgctttccc
    12901 gtttcgtttt ttgcccctcg cctgtgctga tggcgtttgc caaagtagcg ccaaccaaat
    12961 caaggagcga aacagatgga caagcaacgc ggttttacac ttatcgaact gatggtggtt
    13021 attggcatca ttgccatttt aagcgccatt ggtattcccg cttatcaaaa ctacctgcgc
    13081 aaagccgcac tcaccgacat gctacaaacc tttgtgcctt accgtaccgc cgtagagttg
    13141 tgcgcgctgg aacatggtgg attagatacc tgcgacggtg gcagcaatgg cattccctcg
    13201 cctaccacca cccactatgt ttcagccatg agtgtggcaa aaggcgtggt gtcgctgacc
    13261 gggcaagaaa gtctcaatgg actaagcgtc gtcatgacac cgggttggga taacgcaaac
    13321 ggcgtcaccg gctggacgcg caactgcaat attcaaagtg acagcgcatt gcagcaagcc
    13381 tgcgaagatg tcttccgctt tgatgacgcc aactaaggag cggcaatgaa tattccacag
    13441 ctcacggccc tgtgtctgcg ttatcaggga gtcttgctgg atgccagcga agaagtggtt
    13501 catgttgcgg tggtcgatgc cccctcacat gagttgctgg acgcattgca tttcgctacc
    13561 accaaacgta ttgagatcac ctgctggacg cgccaacaaa tggaaggtca cgccagtcgc
    13621 acacaacaga cattgcccgt agctgttcag gagaagcatc agcccaaagc agagttgcta
    13681 gctcgaacgt tacaatctgc gctggaacaa cgcgcgtctg atattcatat cgaaccagcg
    13741 gacaatgcct accgcatccg cttgcgtatc gacggcgtat tgcatccttt accggatgtt
    13801 tcaccggatg ccggagtcgc attaaccgcc agattaaaag tgctgggaaa cctggatatt
    13861 gcggaacatc gcctgccgca ggacgggcaa ttcactgtcg aactggcagg aaacgccgtc
    13921 tcatttcgta ttgcgacctt accatgtcgg ggtggtgaaa aggtggtatt aaggttgtta
    13981 cagcaggtgg gtcaggcact ggatgtcaac acgcttggaa tgcagccgtt acaactggcg
    14041 gactttgctc atgccttgca acaaccacag ggactggtgc tggtaactgg ccctaccggc
    14101 agcggcaaaa cggtcacgct ttatagtgcc ctgcaaacgc tgaataccgc tgacattaat
    14161 atttgtagcg tcgaagatcc ggttgagatc cccatagccg gactaaacca gacgcaaatc
    14221 catccgcgtg ccgggctcac ctttcagggc gttttgcgtg cgttattgcg ccaggatcct
    14281 gacgtcatca tgatcggaga gatccgcgat ggcgaaacag cagagatcgc tattaaagcg
    14341 gcgcaaactg gtcacctggt gttgtctacc ctacacacta attccacctg cgaaacgctg
    14401 gtacgtttac agcaaatggg agtcgcccgc tggatgctct catcagcgct tacgctggta
    14461 atagcccagc gtctggtacg taaactttgc ccacattgtc gccagcagca aggggagccc
    14521 atccatattc cagtcaatgt atggccgtcg ccgctgcccc actggcaggc acccggttgt
    14581 gtacattgct accacggttt ttatggtcgt acggccttat ttgaagttct gcccataacg
    14641 ccggtcattc gtcagcttat ttccgctaat accgacgttg aatcgctgga aacgcacgca
    14701 cgacaggcgg gtatgtgtac gctttttgaa aacggctgcc tggccgtgga gcaaggctta
    14761 accacctttg aagagttaat ccgcgtactg gggatgccgc atggcgagta agcaactctg
    14821 gcgctggcat ggcataaccg gcgacggcaa tgcgcaagat gggatgctat gggcagagag
    14881 ccgtgctttg ctgctcatgg cactacagca acagatggtt accccactta gcctgaagcg
    14941 aatcgccatc aattctgcgc agtggcgagg agataaaagc gcggaagtca ttcatcaact
    15001 ggcgacgcta ctcaaagccg ggttaacgct ttctgaaggg ctggcactgc tggcggaaca
    15061 gcatcccagt aagcaatggc aagcgttgct gcaatcgctg gcgcacgatc tcgaacaggg
    15121 cattgctttt tccaatgcct tattaccctg gtcagaggta tttccgccac tctatcaggc
    15181 gatgatccgc acgggtgaac tgaccggtaa gctggatgaa tgctgctttg aactggcgcg
    15241 tcagcaaaaa gcccagcgtc agttgaccga caaagtgaaa tcagcgttac gttatcccat
    15301 catcatttta gcgatggcaa tcatggtggt tgtggcaatt ctgcattttg ttctgccgga
    15361 gtttgccgct atctataaga ccttcaacac cccactaccg gcactaacgc aggggatcat
    15421 gacgctggca gactttagtg gcgaatgggg ctggctgctg gtgttgttcg gctttctgct
    15481 ggcgatagcc aataagttgc tgatgcgccg ccctacctgg cttatcgcgc ggcagaaatt
    15541 gttgttacga atcccgatta tgggttcact gatgagggga caaaaactca cgcagatctt
    15601 tacgattctg gcgctgacac aaagtgcagg cattactttt ttacagggcg tagagagcgt
    15661 cagagaaaca atgcgctgcc cgtactgggt gcaacttctg acacaaatcc agcacgatat
    15721 cagtaacggt catcccatct ggctggcgct aaaaaatgcc ggtgagttta gcccgctctg
    15781 tttgcaatta gtgagaacag gagaggcatc cggctctctg gatctcatgt tagacaacct
    15841 cgcccatcat catcgggaaa acacaatggc gctggcggat aacctcgcag ccttactgga
    15901 accggcgttg ctgatcataa cgggaggaat tattggtacg ctggtggtgg caatgtatct
    15961 gccaattttc catttaggcg atgcgatgag tgggatggga taacgctggc gtgttgctcc
    16021 acgccagcgt tgggagatta caggttgttg aagatgcggt tttcttgttc ctgcacacga
    16081 ataaacgtgg tgcgcttggt cagctctttc aggcgtgaag ccccaacgta tgtacaagct
    16141 gaacgcaggc cgcccaaaat atctcgcgcg gtattttcaa ccgggcctcg cagcggcagc
    16201 ttaacggttt taccttctgc tgcgcgatat tccgcaacgc cgccaacgtg acgtttcatc
    16261 gcagactcgg agctcatgcc gtagaacagc ataaatttct cgccgttctc ctcaacgatg
    16321 cgaccgccgc tctcttcgta gcccgccagc atgccgccaa gcatgacgaa atcggcaccg
    16381 ccgccaaagg ctttcgccac atcgcccggc gtggtgcagc caccatcgct gacgatcatt
    16441 ccgcccagac cgtgcgcagc atcggcacat tcgattaccg cagaaagttg cggataaccg
    16501 acgcctgttt tgacgcgagt tgtacaaaca gaacctgggc caatgccaac tttaacgata
    16561 tcggcacctg agaggataag ctcctcacac atttcaccag tcactacgtt accagcacaa
    16621 atggttttgg tcggccacgc ttcacgcgct ttcgcaacga actgcacgaa gtgttcggaa
    16681 taaccattcg ccacgtcaat acaaacgaag tttaatgccg ggttcaggtc gagaatctgt
    16741 ttagtttttt cgaaatccgc atcagacgta ccggtagaaa ccatcacatg tttcagcaca
    16801 tcagcggaag aattgttgat aaacgcttgc cactcttcga cagaatagtg tttatgcaca
    16861 gcagtcaaaa tatcaaaaga agccagcgca gaggccatag aaaatgtgcc tacggtgtcc
    16921 atatttgcgg cgataatcgg cacgccggac cagctctgac ctgaatgttt gaaggtgaat
    16981 tgacgttcca gttcaacatc ggaacggctt ttaagagtgg agcgtttagg gcggatgaga
    17041 acgtctttaa aacctaactt cagatcttct tcaatacgca tgtgcggatt cctggggtta
    17101 atggctataa gggtaagagc gcaactccag tgacgctatc atacgcacta atcaatgcgg
    17161 cgcaagactg cgaaattgtc tttttttacg ctacaatccc ataaatttac ctggtaaata
    17221 gtgagcgaac gtttcatcaa tttcgctttc gtgcactcat ttttaacgtt ttgataatca
    17281 tgatgaattc ccgggaatat aaattatgag gtatatagtt gccttaacgg gaggcattgg
    17341 cagtggcaag agtaccgttg ccaatgcgtt tgctgatctc ggaattaacg tcattgatgc
    17401 cgatattatt gcgcgtcagg tggttgaacc aggtgcacct gcgctacatg ccattgctga
    17461 tcactttggc gctaacatga ttgctgctga tggaacattg cagcgccggg ccttgcgcga
    17521 gcggatcttc gccaacccgg aagagaaaaa ctggcttaac gccctgctgc atccgctgat
    17581 tcagcaagag acgcaacacc agatccagca agctacttcc ccctatgtac tgtgggttgt
    17641 gccattgctg gtagaaaact cactgtataa aaaagcgaat cgagtgctgg tggtggatgt
    17701 cagcccagaa acgcaactta agcgcaccat gcagcgcgat gatgtaactc gcgagcatgt
    17761 cgaacaaatc cttgctgctc aggcaacgcg cgaagcccgc cttgccgtgg cagatgacgt
    17821 cattgataat aacggcgcac cggatgctat cgcatcggat gttgcccgcc tgcacgcaca
    17881 ctatttgcag cttgcgtcgc agtttgtctc acaggaaaaa ccgtaatgca gacccaggtc
    17941 ctttttgaac atccactaaa tgaaaaaatg cgtacatggc tgcgcattga gtttttgatt
    18001 cagcaactca ccgttaattt acccatcgtt gaccacgctg gcgcgctgca tttcttccgt
    18061 aatgtcagtg aattactgga tgttttcgag cgcggcgaag tccgcactga gctgttgaaa
    18121 gaacttgacc ggcagcaacg taaactccag acctggattg gcgtgcctgg cgtggaccag
    18181 agccgtattg aagcattaat tcagcagtta aaagcggcgg ggagcgtatt aatttccgcg
    18241 ccgcgtatcg ggcaatttct gcgtgaagat cgtttgattg ctctggtgcg tcagcgactg
    18301 agcatcccag gcggctgttg cagctttgat ttacctacat tgcacatttg gctgcatcta
    18361 ccccaggcgc agcgcgacag ccaggtagaa acctggattg ccagcctgaa cccgctcacc
    18421 caggcactta ccatggtgct ggatttaatt cgccagtcgg cccccttccg taaacaaacc
    18481 agcctgaatg gtttttatca ggataacggt ggcgatgccg acttgctgcg cctgaatctg
    18541 tcgctcgatt cacagcttta tccgcaaatt tccggacata agagccgttt tgccattcgt
    18601 tttatgccgc tggacactga aaacggacag gtaccggaac gtctggattt cgaactggcc
    18661 tgttgctaag gagtaaaaat gtcagaaact attacggtga attgcccaac ctgcgggaaa
    18721 acggtggtgt ggggtgaaat cagcccgttt cggccatttt gctccaaacg ttgtcagctg
    18781 atcgacctcg gagaatgggc tgctgaagaa aaacgaatcc ccagcagtgg cgatctttcc
    18841 gaaagcgatg actggagcga agagccaaag cagtgacatt tgtctgatgc cgcacgtagg
    18901 cctgataaga cgcggacagc gtcgcatcag gcatcttgtg caaatgtcgg atgcggcgtg
    18961 aacgccttat ccgacctaca aaaacaaatt tatcaatgag ttagaaatcc ctgtaggcct
    19021 gataagacgc gccagcgtcg catcaggcat cttgtgcgaa tgtcggatgc ggcgaaaacg
    19081 ccttatctga cctacagacg tttaagcttc gcaattaccg gttcattggc tggcggaaaa
    19141 tcatcggcat taagatcgac cagcgccatc cactcaccgg gttgcccttc tttaccccac
    19201 ggcttccctt cccagcgttc gaccagccag aaccacagtg ttatatgcct gtccgggaat
    19261 tcatattcca gtttttcaaa tagcgaaaaa tgttgtgggg taatcccgac ttcttcctga
    19321 agttcacgca ccaccgcctg ttccggcgtt tcacccattt caattttacc gccgggaaac
    19381 tccagtttat tcgccatgtg cgcatctgct gcgcgacgcg ttataaagat ttcattgttc
    19441 tcgttgcgaa taatacctac cgcaatttgc agctttttca ttgtcttaaa cctataaaaa
    19501 aggcgcagaa tcctgcgcct tttacttcaa ctgttagctt ttattgcagg cgaccatggc
    19561 actgcttgta ttttttacca gaaccgcacg ggcaaggatc gttacgtcct actttgcgtt
    19621 caccggtttg cgccgccagt gcagctgcgg ctgcagagtc gtcatcctga tggctaagct
    19681 gctgcatttg cgctaaacgc tcggcttcca tacgacgctg ttgttccagc tcctcaactt
    19741 cttcaggcat acgtacctga actttgctca gcgtactgat aacttcatat ttcaacgact
    19801 ccagcatcgc tgcaaacatg gagaacgatt cacgtttgta ttcctgcttc ggatctttct
    19861 gtgcatagcc tcgcaggtgg ataccctgac gcagatagtc catcgctgcc aggtgctctt
    19921 tccacaggga gtcaagcgtt tgcagcatga cgcctttctc gaagtgacgc atcatctctg
    19981 caccaaccac ttcttcttta cgctgataca cttcgatgga ctgcgccaga atgcgctcac
    20041 gcagcgtctc ttcatgcagt tcgggttctt tatccagcca ctcagcaatt ggtaaatcga
    20101 ggtcgaaatc gttcttcaga cgttcctgca gccccggaat atcccacatt tcttccagcg
    20161 actgcggcgg aatgtaggca tcaatggtgg ctttgaacac atcttcacga atgctgttga
    20221 tggtttcgct cacatcgcta acatccagca gttcgttacg ctgggagtaa atggcgcgac
    20281 gctgatcgtt agccacgtca tcatattcca gcagttgctt acgaatgtcg aagttacgac
    20341 tttcaacttt acgctgggcg ttggcaatcg ctttggtcac ccacgggtgt tcaatggctt
    20401 cgcctggctt catacccagt ttacgcatca tgccggatac tcggtcggaa gcaaaaatac
    20461 gcatcagcgc atcttccatc gacaggtaga aacgggaaga accagcatcc ccctgacgac
    20521 cagaacgacc gcgcaactgg ttatcgatac gacgggattc gtgacgctcg gtaccgatga
    20581 tatgcaggcc acctgctgcc agtaccgcat cgtgacgtac ctgccagtcg gctttaattt
    20641 tttcaatttg ctctgcggtc ggattttcca gcgcggcaac ttctgcctgc cagctaccac
    20701 cgagcacaat atctgtacca cgacccgcca tgttggtcgc gatagtcacc gcagccggat
    20761 aacctgcctg agcaacaatc gccgcttcgt tggcgtggaa tttggcgttc aggacattgt
    20821 gcttaatacc ggctttggtc agttcgtttg acaccagctc cgatttttcg atggagatag
    20881 tacccaccag caccggctgg cctttcgcag tacgttcttt gatatcttca atgatcgcct
    20941 gaattttttc cgcttcagtc atgtagacca ggtccggcaa atctttacga atcattggac
    21001 ggttggtcgg aacaacgacg gtatccagct tgtagatgga gctaaattcg aaagcttcgg
    21061 tatcagcagt accggtcatc cccgccagtt tttcatacag acggaagtag ttctggaagg
    21121 tgatcgaagc cagcgtttgg ttttcgttct ggatctgcac accttctttc gcttccacag
    21181 cctggtgcag accatcggac cagcgacggc cctgcatggt acgaccggtg tgttcgtcaa
    21241 cgatgataac ttcaccatct ttaacgatgt agtcgacgtc gcgggtaaac agcgcatgag
    21301 cgcgcagcgc cgccgttacg tggtgcatca gcatgatgtt ggccggagag tacagagact
    21361 ccccttcatc catgatgccc tctttcacca gcagttcttc aatcagcacc agaccacgtt
    21421 cggtcaggtt cacctggcga gatttttcgt ccaccgagaa gtggccttcg ccctggaagg
    21481 tttcggagtc ttctttttcc tgacggatca ggtgcggaat aattttattc acgcgtttat
    21541 acatttccga gctgtcttct gccgggccgg aaatgatcag cggtgtacgc gcttcatcga
    21601 tcaggatgga gtccacttcg tccaccagcg catagtgcag tttacgctgt acacgttctt
    21661 cagggctgaa cgccatattg tcgcgcaggt agtcaaagcc gtattcgttg ttcgtaccgt
    21721 aagtgatgtc agctgcgtaa gcttcgcgct ttgccggtgc tggcatgccc ggcaggttga
    21781 taccgacagt caggccaagg aattcaaaca gcggacggtt gttttcggcg tcacgttgcg
    21841 ccaggtagtc gttgacggta actacgtgca cgcctttacc ggttagtgcg ttcaggtaag
    21901 caggcagcgt tgcggtcagg gttttacctt caccggtacg catttcggcg atgcagcgtt
    21961 cgttaagaac cataccgccg agtaactgaa cgtcgaagtg acgcatacca aagacgcgct
    22021 tacttgcctc acgtaccacg gcgaaagctt ccgggatcag attttccagc acttcgcctt
    22081 tttccagacg tgcacgaaac tctgcggttt tccctttcag ttcttcgtcg gagagttttt
    22141 ccatctccgg ttccatggca ttgatgatgt tgaccacttt gcgcatccgg cgcagggtgc
    22201 gatcgttacg actaccgaaa actttagtta acaatttgat tagcataata aaatctcaaa
    22261 cgccccgcgt tgcggagtta ataaaatgaa gtaaaggttt attgttgtta ggtgaggcgt
    22321 tgagggccag cacggatgcc ttgcgcctgg cttatccaga cgggcgtgct gaattttgct
    22381 tgtggggtaa aatgcgcata atcaatgcga taaccctttt cagacggcgt gccttcctgg
    22441 gtcagcagcg cgctgagcgt atccagtaat gcaagatgtt gcgcctgaag aggcaaagat
    22501 tcttcagcaa cgggcagtgt ttgcggtgcc attgcgaaag aaagatgacg gattaccgtg
    22561 cgaatggcat gttgatgcca gtaatcaacg gaatagttcg aattcgggcg gcgtgtgttc
    22621 gcttccagca aggccaattg accaaagtta actttggctg aaggctcgtg gttgcgggtt
    22681 gtcgctttta cgggcgcgtt tggttcggcg gcgttgctga gcgcaggcaa acctaaactc
    22741 gccgcaacca tccctaataa gagatgcggc cagaagtagc gtttaccaaa ctgtcgccag
    22801 cgcgtcagta ttccactcac gttattgcca tcccgatcgt attcaaacgt atttagcgca
    22861 caaatcttat cattaaagcc tgcagtccac agcacgaatg aagataagtt gtgcggaaaa
    22921 gtgcttaaga aaacagcgtt cgcaccgttt tttatagctc ttataaaatt caaaaaaaaa
    22981 cgactggttc acctggccgg agagagtgcc agatttgcca gtcgaatttt atacgacagt
    23041 ataaatgtcg ttatgccagt acagctgaag gcgctttgaa ggccaacggc agttctgcgt
    23101 cgtcctggaa ggtcacatat tcccaggctt cctgtttcgc caggacagcc tgcagcagtt
    23161 tgttattcag tgcatgaccg gatttataag cggtaaatgc accaataata ttgtgaccac
    23221 acatgaacaa gtcaccgatc gcatcgagca ttttgtgacg cacaaattcg tcttcaaaac
    23281 gcaggccgtc ttcgttcagt acgcgataat cgtcaacaac gatggcacaa tcgaagctgc
    23341 cgcccaggca caaaccacgg gactgcagat attcgatatc acgcatgaaa ccgaacgtac
    23401 gcgcacggct gatctggcgc ataaacgcat cagcggagaa gttcatcgca tagcgctggt
    23461 tgctggaatc aatagccgga tggttaaaat cgatggtgaa atccagcgaa aaaccattgt
    23521 acggcttaaa ttcagcccac ttatcgccat cttcgacacg aacagtctct ttgatgcgaa
    23581 caaatttttt ggcgcagttc aactcgtcga taccggcgtc aagcagcagg tatacaaacg
    23641 gagcggcgct gccgtccatg atcgggattt ccggcgcgtt aacttcgata acaatgttat
    23701 cgatgcccag gcccgcgaga gcagcattga ggtgctctac ggttgaaatc cgtacatcat
    23761 gctcgttgac cagacacgta cagagcatgg tatcacgcac agatttggca tcggccggga
    23821 aatctaccgg tggattcaag tcggtgcgac gatagatgac cccggtgttg gccggcgcag
    23881 ggcgtaacgt cagggtgact ttcttgccgg tatgtaaacc gacacccgtc gcctgaacga
    23941 tacgtttaag tgtcctttgt ttgatcatcg tattatctcg ccaaattacc tatccaaccg
    24001 aagtgtacta tacattcggc gggccagttt agcacaaaga gcctcgaaac ccaaattcca
    24061 gtcaattctt aatcagcttg cttacgcagg aatgctggga tatccagata atccggctct
    24121 ttcgcagttt gcggcgcatt gtcattcacg actttagcaa ctggcttctg ctcctgggtc
    24181 agcggagcca tcccatgctg ctggtagcga tccatcactg gctgctgaac ctgcttattg
    24241 gtcaccagag tgatttcagg acgtttgtcc atgccgatac ctgtcgcaac aacagttacg
    24301 cgcagctcgt cattcatatc cgggtcaaga gaagtaccga taaccacagt cgcgttgtcg
    24361 gaagcaaatg cacggatggt gttacctacc gtttcgaact catccagacg caggtcgaag
    24421 cccgctgtga tgttaaccag cacgccgcgt gcgccagata ggtcgatatc ttccagcagc
    24481 ggagaagaaa tagccatttc agcagcttct tccgcacggt cttcaccgct cgccacgcca
    24541 gaacccatca ttgcgtagcc catttcagac attacggtgc gtacgtctgc aaagtccacg
    24601 ttcatcaaac ccggacgagt aatcagttcg gcgatgccct gaacagcgcc tttcagtaca
    24661 tcgttcgctg cgccaaacgc atccagcagg gagataccgc ggcccagaac tttcagcagt
    24721 ttgtcgttcg ggatagtgat cagagagtcc acatgcttgg acagttcagt gatcccctgc
    24781 tccgcgaatg ccatacgctt cttgccttca aagttgaaag gcttagtgac gacagcaacg
    24841 gtcaggatac ccaaatcttt tgccacttca gcgacgactg gtgctgcacc tgtaccggta
    24901 ccacccccca tacccgcagc aataaagacc atgtctgcac cttccagcgc cgcacgcagt
    24961 gcatcgcgat cctcatcagc cgcattgcgg ccaacttctg gattagcgcc agcgcccagt
    25021 cctttggtga taccgctacc gatttgaatc gtctgtccaa ccgctgtttt acgcagcgct
    25081 tgtgcatcgg tatttaccgc gaagaattca acaccttcaa tacgctcgcg caccatgtgt
    25141 tcaacagcat taccgccgcc gccgccgacg ccgatgactt taatcaccgc gtcattggta
    25201 agttccattg gttcaaacat agtttctctc cgatttgtgc ctgtcgcctg aggccgtaat
    25261 catcgtcggc ctcataaaaa ttaaaactct tttcgcagcc aactattgag tcgcttgatc
    25321 cacgagccaa ctgatgctgt aacacgtttt tctacttcag cttcaccgtt aagatgtgac
    25381 tctttcccat agtgaagcaa tcccaccgcc gtcgaataat acggctcctg agcataatcc
    25441 gttaaaccgg taatgttcag cggcgcgccg atacgcactt gcgtatgaaa cacgcgctga
    25501 gcacaggctg caagaccttc gatctgcgct gcgccaccgg ttaatacaat gcctgccgcc
    25561 aggtgatgtt taaccccttg ttggcgaagc ttttcctgca actgcaatat ctcttcgttg
    25621 accaggttga gcagctcggt atagcgcggc tcgatcacct ctgccagggt ctgacgttgc
    25681 agactccgcg gcggacgacc acctacgctc ggcacttcca cgctctcatc ttttccaacg
    25741 atggaaccca gcgcacaacc gtggcgaact ttaatcgctt cggcgtcgct tggcggcgtg
    25801 ccaaaggcgt aagcgatatc actggtcacg acattgccag cataaggaat taccttagtg
    25861 tggcgcaatg ccccaccggt ataaacggcg atatccattg taccaccacc gatatcgacg
    25921 acgcagacac ccagttcacg ttcatcttcc gtcaataccg aataacttga tgccagtccg
    25981 gcaaatatca gttggtcaac tttcagccca caacgttcaa ccgctttgac gatgtttttc
    26041 gccatatcgt tgtgacatgt gatcaggtgc acttttgcct gcatccgcac gcccgaaagt
    26101 cctaccggat tcttgatccc ttcctgatag tcaatcgcat actcttgcgg gatcacatgc
    26161 agcacacgat gctcatcgcg cacacgcacc gatttcgcgg tatggacgac gttttccaca
    26221 tcttcttgcg tcacttcttc ttcagaaata ggcaccatac caatttcatt ctggcagctg
    26281 atgtgcttac cagaaagcgc cagatatacc gaagagatct gacaatctgc catcaattct
    26341 gcctggtcaa tggcgcgttg tacgcacttg accacggatt cgaggtcgtt caccccgcct
    26401 ttatccatac cacgcgacgg gcagctgccc acgccaatga tattgaccat accgtcgggc
    26461 agaacttccc ctactaaagc ggcaaccttc gcggtaccaa tctccagtcc tactaccagt
    26521 tttctgtccg tcgccttgat cattgttgtt ctgcctgtgc ctgattttgt tgctgagtag
    26581 attcctctgg cggcaagggc gcccagccta ctgccgctcc agagtcataa cgcaaatcaa
    26641 cgtagctaat ccgtttgcca tcggtttgcg cctgctgctg taaaaccgga taaagttcta
    26701 caaagcgagc caaacgtttc atcgtatcgc cccggccaag attgagctta atatcgttat
    26761 tcagcgtcaa ctgccaggaa cgccgcgcgg tcatcgccgc ttccttcaga gtaaatctgt
    26821 cctttgccag catctgcccc atttcgcgat agccctgcaa cacttcattg gcgctgcctt
    26881 ccgggccata cagcattgga agcacctgct tgctggtgcg ttctggcggc acgctgaagg
    26941 tatttccttc cgcgtctacc atatgttgat cattccaccg cgcaatcggc acatattcaa
    27001 ccagatgaat cttcaattca tcaggccact gctttctgac gctcacctgc ttaatccacg
    27061 gcaggcgttg ttctatttgc gtctggatga tgttgacatc ctgggtcata aaggtacccg
    27121 gctcacccaa tgccaggatc gactgccgga tatcgtcatt acgtgtgtaa tggcgttcac
    27181 cggtcaacac cagctttgag agcggcaggc gttgcgcatc ttccatccag cccaacacga
    27241 cccagccgct caccaacact gtcgttaaaa cggtcagcag gaaaaggatc cccgccagac
    27301 gcgttccatt attgcggcga gaagaaacct cttcttcgct gtttcgcgtg ttcagagcag
    27361 cctgcgacat attagtccgc cagttccaga attcgtacta ccaactgcga gaagctcata
    27421 cctgcctgac gtgccgccat cggcaccagg ctgtggctgg tcatacccgg tgaggtattg
    27481 gcttccagca gataaaactg tccatcgctg tccagcataa cgtcaatacg tccccatcct
    27541 ttgcaaccta acgtcgtcca tgctttcagc actaatgcct gcaaattggc ctcttgtgac
    27601 gcttccagac ctgcggggca gaaatactgt gtctcatcag agagatactt cgcctcataa
    27661 tcatagaagg ttccggacgg ttgaatacgt attgacggta aaatttcttc accgagtatc
    27721 gcaaccgtga actccggccc acttagccat ttttcaatca atacttcttc atcgtgctga
    27781 aatgccaatc ttaatgcatc ttgtagagca ttttctgcta ctacttttga cattcccaca
    27841 ctggaacctt cgcggctcgg cttaacgata accggcaaac ccagagcaga aatttctgct
    27901 aactgcttat cgctcaggcc tttttcaaac tctgcgcggg ttaacgctac ccacggcgcg
    27961 accggtaaac cggcaccttg ccatagaagt ttgctgcgta gtttatccat tgaaagcgca
    28021 gatgccatca ctccgcttcc ggtataaggc aagcccatca gctcgagcat cccctgcagc
    28081 gtaccatctt caccgccgcg accgtgtagc gcgataaaca ctttctgaaa gcccatcgac
    28141 ttcagttgcg tcacgtcgac ttctttcggg tcgacaggat acgcgtcaat accgccttca
    28201 cgcagtccgg ctaacaccgc tgcgccagaa ttcagagaaa cttcccgctc agcggaggtc
    28261 ccacccaaca ggaccgcgat tttatcagtc atgttgttct tcctccggag tttgcggctt
    28321 cagtttgatt tcagctaaag aacgagcaat ttttccaata ttaccagccc cctgaacgag
    28381 aatcaggtcg ttaccggtta ataccggtgc cagcatctcg gctacccgcg ccggatccgg
    28441 caccagaatg ggatcaattt tcccacgtcc acgaattgtg cgacacagcg aacggctgtc
    28501 cgctcccgga attggcgctt cgccagccgg atacacttcc agcatcaaca gggtatcaac
    28561 ctgcgtcagc acattggcga aatcatcata caggtcgcgc gtacgggtaa aacggtgcgg
    28621 ctgaaacagc attaccaggt ttttatccgg ccagcctgcg cgcgccgctt taatggtggc
    28681 gtccacttca gtcgggtggt ggccgtagtc atcgaccagc attgccgtac cgcttttacc
    28741 attcactggc tccagcggga attcaccgag gaaatcaaaa cggcgaccag tcccctggaa
    28801 gctttcaagc gcccgcaaaa tagcctcgtc gtcaatgccc tcttccgtag caaccgcaac
    28861 cgcagctgcg gcgttcagcg cgttatgacg acctggcgca ttcagggtga cgcgcatcgg
    28921 ctctttgtcc tggcgcagca gcgtaaagtg cccctgcggg ccaatctgct gataatcttc
    28981 tacacgcacg tcggcatctt cgctgaagcc gtaagtcgtg gtctgacgcc ccactcgcgg
    29041 taacaattcg cggatcaccg gatcatcaac acacatcacc gcacgaccgt aaaacggcag
    29101 gttgtgcaga aaattaataa aagtctgttt taaattctca aagtcgccct ggtaggtatc
    29161 catgtggtcg gcttcgatat tggtgacaat cgccaccatc ggttgcagat gcaggaacga
    29221 tgcatcactc tcatctgctt cggcaatcag gtaccgacca tgccccaaac gcgcatgaac
    29281 ccccgccgct tttaccagcc cgccgttaac gaaggttggg tcgagccccg cttctgcgta
    29341 gatgctggaa accatcgcgg tagttgtcgt tttgccgtgc gttcctgcaa tggcgatgcc
    29401 atgacgaaaa cgcattaact cagccagcat ttcggcacga cggatcaccg gaatacgcgc
    29461 ttcatgagct gcgacaattt ccgggttgtc ggcagaaatc gcgctggaaa caacgaccac
    29521 gctggcatca cgtacgtttt ccgggcgatg gttgaaataa atcgtcgctc ccagattcat
    29581 taactgctgc gtgaccggat ttggcgctaa atcggaacca ctgatctgat aaccttcatt
    29641 ggccagaact tcggcaatac cgcccatacc ggcaccaccg atgccgacaa aatgtatgtg
    29701 ccgaacgcga cgcatttcgg gcacgatgga acgcagtttt gccaattgtt gtgtattcat
    29761 tctttacgcc attaacttct taaattcata cgatgcaaaa ggcatcgcta caattacgcc
    29821 cgggcagccc ggctcacttc atttgccact cgctcggtgg catccggaat ggatgcagcg
    29881 cgggcgcgtt ctgccatggt taataaggtt tctcgcgacc acccggccag ggtgttggcg
    29941 acagcatcca cgctaagctg tggctgttcg ataattttgg ctgcgcccgc tttttccagc
    30001 ggtagcgcat tccagtattg ctggcggtct ttatgttgaa acggcacaaa caacgccggt
    30061 agtcctgccg cggcgatttc actcaccgtt aacgcaccgg agcggcaaac gacgacatcc
    30121 gcccacgcat acgccgccgc catatcatca ataaattccg tcactttatg ctgcggttgc
    30181 cccgcttcgg catacgcctg ttcaacggat tgttgcgaac ctttgccgct ctgatgccag
    30241 atagtgactg aatcacccag tttcgcagca acctgcggca ttgtctggtt aagaatgcgt
    30301 gcgccctgag acccccccac taccagcaca cgaaccggac cttcacgtcc agccaaacgt
    30361 tgctgcggca acggcagcgc caacacatcg gtacgcaccg ggttacccac tacttccgca
    30421 ttagggaaag cacctggaaa cgcctgcatc actttggtgg caatcttcgc cagccattta
    30481 ttggttaagc ccgcaatacc gttttgttca tgaagtacaa ccggaatgcc taacgaccac
    30541 gcggccagac cacctggacc tgacacatag ccgcccatac cgagcaccac gtcaggtttg
    30601 tacgctttca taatcgcccg cgcctgacgc caggcattga agatacgcag cggcgcagct
    30661 atcagtgctt ttataccttt tccacgcaga ccagagatac gaatgaaatc aatttcgatg
    30721 ccatgttttg gcactaagtc cgcttccata cggtcagcag tccccagcca gcgaacttgc
    30781 caaccctgag ccattagatg gtgcgcaacc gccagtcccg ggaatacatg tccaccggtt
    30841 ccgcctgcca tcaccattaa tcgctttcct tgaccactca tcgtgaacct cgtacaaacg
    30901 cctgcgcttt ctccagacgc gtttcataat caatacgcaa cagcatcatg atggctgtcg
    30961 acataatcag taagctcgaa ccaccgtaac tgatcagcgg caatgtcaga cctttggtcg
    31021 gtaacatccc cgccgccgcg cctacgttaa ccagcgcctg gaagctaaac cagatgccaa
    31081 tggaacaggc gagaaaaccg gaaaaacggt ggtcaatttc taacgctttg cggccaatcg
    31141 acatcgcacg aaaagcgacg aagaatacca ttaaaagtgc cagcaccaca ccgacatacc
    31201 ccagttcttc gccgataatg gcgaaaataa agtcagtgtg cgcttccggc agatactcca
    31261 gtttttgtac cgagttacct aaaccttgcc cccaaagttc gccgcgacca aacgccatca
    31321 gcgattgcgt taactgatag ccgctgccaa agggatcttc ccacgggttc cagaatgcgg
    31381 taacacggcg gatacggtac ggttcggcga gtatcagcaa cacaaccgct gaaatgccca
    31441 taccgataat ggcaatgaac tgccacaatt tcgctcccgc caggaacaac atcgccagcg
    31501 tagtcacaaa caacaccacc accgtaccaa ggtctggctg tgccagcagt aacactgcca
    31561 acaccagaat cacgcccatc ggtttcagga agccgcgcag gttattacgt acttcgtcgc
    31621 ctttacgcac cagatagttg gcgatatagc aaaacagcga cagttttgtc agctccgcag
    31681 gctggatacg cagcaaaccg agatcgatcc aacgcgatgc ccctttaacc gagctaccca
    31741 ctaccagaac gatcatcagc aggatgatag atccgagcag catcgtggca ctgtagcgtt
    31801 gccagaactc catcggcaga cgcagcgtaa tgatcgccag aataaacgcc aaaatcagat
    31861 agacaccatc acgcttcgcg aagaagaacg gatcgttggt taagcgttgc cctatgggca
    31921 ttgacgccga ggtcaccatg ataaagccaa tcgccgcgag gccgaaggtc agccacagta
    31981 aggtgcgatc gtacatgatc aggctgtcgg tatctttttc ccgcgagccc atcacccagc
    32041 cctttagcgc cgtggagatc cagaccagga tactgaatcc tggcaggcgc ggcattttca
    32101 ggcgagggag agataaacgc atcaacctaa ctccttcgcc agacgggcaa actcattgcc
    32161 tcgttgttca aagttcttga actgatcaag gctggcacag gccggggaga gtagaaccat
    32221 atcgcccggc tgaacacgcg gagccagcaa gcgcatcgcc tgttccatag tttcggtttg
    32281 ttctgccact tccgggtgta gcgccgccag ctgcgcgccg tcacgaccga aacaatacag
    32341 acgtacgtta tcgccattca ggtaacgcgc cagtgggcta aagtccgccg atttaccatc
    32401 gccgcccagc aacaaatgca gcgtgccgtc tacgtgcagg ccattcagcg ccgcttccgt
    32461 actgccgacg ttggtcgctt ttgaatcgtt aatccaacgt acgccgttat gctccagcac
    32521 aacttcaaag cgatgcggca gaccagtgaa tgtggttaac gctttcaggc tgctggcacg
    32581 cggtaaccct gcggcatctg ccagtgccag cgccgccagc gcattggtgt agttatgctg
    32641 cccggaaagt ttcatctctt tcacgttcag cactttctcg cctttaaccc gcagccaggt
    32701 ttcgccctgc tgatgattca ggtgatagtc acccatgttg acgccaaagc tgacgcagcg
    32761 ttcatccgca ccgcgaatcg gcattgttaa ggcatcatcg gcattaacca cgcaaacttt
    32821 tgcgttttcg taaatgcgca gttttgctgc acgatactgt tgtaaaccaa acggatagcg
    32881 atccatatga tcttcagtca cgttcagaat ggtcgctgct accgcctgta agctagaggt
    32941 ggtttccagc tggaagctcg acagttccag cacgtacagt tcgcactcat catccagcag
    33001 catcaacgca ggcaggccaa tattgccacc cactccaacg ttaacccccg ccgctttcgc
    33061 catttcaccc actagcgtgg tgaccgtgct tttgccgtta gaaccggtaa tcgccacaat
    33121 cggtgcttgt gcttcgcgac aaaacagctc gatatcgcca acgatttcga ttccggcatc
    33181 agcggcagcg cttaaggatg gatgcgccag tgcaataccg ggactggcga caatcagatc
    33241 tgccgccatc agccattcat cattcagact gcccgtgtgg cgttctacgg cttcgggtaa
    33301 tttatccagg ccaggcggtg tcatacgcgt atccataacg cgcggcgtca caccgcgagc
    33361 gaggaaaaag tccacgcagg aaagcccggt gaggcccagg ccgataatga caacattttt
    33421 accctggtaa tcagccatga ttaacgtacc ttcagcgttg ccagaccaat caggaccagc
    33481 atcagcgaaa taatccagaa acgcacaatg acgcgcggtt ccggccagcc tttcagttca
    33541 tagtggtgat gaatcggtgc catgcggaaa atacgttgtc cgcgcagttt aaaggagccg
    33601 acctgcagga tgacagaaag cgtttctacc acgaacacgc cccccataat caccagcagg
    33661 aattcctgac gtagcagtac ggcgataatg cctaacgcac cacctaacgc cagcgacccg
    33721 acatcgccca taaagacctg cgccggatag gtgttaaacc acaggaagcc cagtcctgcc
    33781 ccgactatcg cggtacagac aataaccagt tccccggcgt gtcgcagata cggtatgtgt
    33841 aaatagctgg caaagttcat gttgccggtc gcccacgcca ccagcgcaaa accaccggcg
    33901 acaaatacgg tcggcataat tgccagaccg tcaagaccat cggtcaggtt taccgcgttg
    33961 ccagtaccca caatgacgaa gtaagccagc agaatgtaga acagccccag ttgcggcatc
    34021 acatctttaa agaatgggac caccagctgc gttgcgggcg tgtctttgcc ggcaaggtac
    34081 agggcgaagg cgacacccag cgcaatgacc gacatccaga aatacttcca acgagcgatc
    34141 aaccctttgg tgtctttacg caccactttg cgataatcat caacaaagcc aataacaccg
    34201 taacctacca gcaccaccaa cacgcaccag acgtacggat tggacgggta agcccacagc
    34261 agtacggaga tcacaatcgc cgtcaggatc ataatcccgc ccatggtcgg cgtaccgcgc
    34321 ttgctgaagt gtgattcagg accgtcgtta cgcaccacct gaccaaagga aagtttttgc
    34381 aaatgagcaa tcatacgcgg gcccatccac aatgagatga acagcgcggt cagcaggctg
    34441 acgatggcgc gaaacgtcag ataggaaaag acgttaaagc cggaataata tttgaccaaa
    34501 tgttcggcca gccaaactaa catgtcccat tctcctgtaa agcgcgtact acctcttcca
    34561 tggcggcact acgtgaaccc ttaactaaaa tcgtaattac ctgttgctca gcaatcagtg
    34621 atttaagacg cgtaattaac gcagttttat cagcaaaatg ttcgccaacg ccgctggcgg
    34681 tgctgatagc atggctttgt ttacccacgc ttaacacgcg gtcaatacca gcagcttttg
    34741 ccgcctcgcc cacctgtaca tggcaggctt cgctttcagc gcccagttcc gccatatcgc
    34801 ccaccaccag cacgcggtag cccggcattt cagccagtac ctggactgct gcagtcattg
    34861 aaccgacatt ggcgttgtag gagtcgtcga gcagcaactg gttttctgcc agttggatgg
    34921 ggaacagacg gcctggaaca gctttcagat ttgccagccc cgctttgata gcatcaagcg
    34981 ttgcgcccac ggacatggag agcgcagcgg ctgccagcgc attcgcaata ttgtgacgcc
    35041 ccggcaacgg cagcagaaca tcgacgctac cggttggggt ttgtagggta aattccgtac
    35101 cgtgcgaggt cacatggata ttggtggcgg tgaaatcgct gttggcggca ttgggtgaga
    35161 aacgccacac tttgcgtgag ccaattacgc tctgccagtt cagccagtcg ttgttgtcgg
    35221 cgttcataat ggcgataccg ttttccggca ggccgctaaa gatttcacct ttcgctttcg
    35281 cgacacccgc aagcgagcca aaaccttcca gatgcgccgc tgccaggttg ttgaccagcg
    35341 cagcttccgg gcgagtcaga ctcacagtcc aggctatttc gccctgatgg ttcgcgccaa
    35401 gttcaataac tgcgtaatcg tattccggcg ttaagcgcaa cagcgtcatc ggtacaccga
    35461 tgtcgttgtt gagattgcct gccgtataaa gcgtgttgcc gcactggctt aaaatcgccg
    35521 ccgtcatctc tttaacggag gttttgccgg aggaccccgt cagagcaacc acgcgcgccg
    35581 gaacttgctg gcgaacccat gcagccagtt caccaaacgc cagacgcgta tccttgacga
    35641 ttaactgcgg caggtcgatg tccagcggac ggctaaccag tagtgcgcct gcgccgccag
    35701 ctttcgcctg gtcggcaaaa tcgtgggcat caaaacgttc gcctttcagg gcaacaaaca
    35761 ggcagcccgg cgtcagtttt cgggtatcag tggttacagc atcaagggtg atatctgcac
    35821 cttgcagttc accgttgaga atgtcggtaa gttggctaag ggttacgcta atcatgcaat
    35881 cacccccagc agacgcgcca ccgtgacgcg atcggagtag tccagacgct gattgccaac
    35941 aatctggtaa tcttcatggc ctttgcccgc gaccagtacc acatcattct ctttagcctg
    36001 cataacggcg caagtcaccg cttcagcacg gccttccatc actttggcat gtccggcatc
    36061 taacattccc gccagaatat cgttgatgat ggcacgcggt tcttcggtac gcgggttatc
    36121 gtccgtcacc accgccacgt cagcaaactc ttcggcaatt gcgcccatca gtggacgctt
    36181 acctttatcg cgatcgccac cacagccaaa gacgcaccac agcttgcccg cacagtgcag
    36241 gcgcgccgcc tgtaaggctt tttccagtgc atccggcgta tgcgcgtaat ccaccaccac
    36301 cgtcggtttg cctggcgcag tgaacacttc catacgtccg caaaccggtt gcagacgcgc
    36361 ggcggttttt aacagatcag ccagcgggta gccaagtgcc agcagtgtcg ccagcgcgag
    36421 cagcaggttg ctgacgttaa aagcgcccat cagatggctt tcaatttcgc catcgcccca
    36481 acttgaacta aagcgaatgg tcgcgccgct gtcgtgatag ttcacttcgg tcgctttcaa
    36541 ccagcgtccg tgacagttcg gattgatatg atcttccatt gataccgcaa ccgcgtccgg
    36601 cagttttgcc agccagcggc ggcccacttc atcgtcggcg ttaacaatcg cctgaccaca
    36661 atgatgttca gaataaagca gccatttcgc ggcttcgtag tgttccatat caccatgata
    36721 atcaaggtga tcgcggctta agttggtaaa gaccgacgcc gcaaatttca atgccgccac
    36781 acggtgctgt accagcccgt gggaggaaac ttccattgcg caaaacgtcg cgccctgatc
    36841 caccagcccc gccagctcat gctgaacatc gactgccgaa ccggtggtat tttctgtcgg
    36901 gatcactttc cccagcagac cgttaccaac ggtgcccatt accgcgctgg tttcgccaag
    36961 cagttggctc cactgcgcca gcagctgggt agtcgtggtt ttgccgttgg tgcccgttac
    37021 gcctacgaga cgtaaattgt cagagggttc atggtaaaag cggcccgcca gtgcagataa
    37081 acgctcgttg agctggctga gatagatgac cggtacgccg tgcatttcac ggatttcacc
    37141 gtcggtcgcc tcacctttcg cctctgcaat aatggcagcc acaccttgcg ctatcgcctg
    37201 cgggatatat cgacgcccgt ccgcctgatg acctactaca gctacaaaga gatcgcccgc
    37261 cgcagccaca cggctgtcga gtgtcatctc tcgcagtgct cgcgaaggtg cgtctggcac
    37321 ccacggagca agaaggtcgc gcaaattacg atctgccacc tgtcccctcg ccttgattaa
    37381 tcacaaattc atttttatcg cccgttgtca gcgcatccgg ctcgatgttc atggtacgca
    37441 atacgccgcc catgatggca ccaaagaccg gcgctgaaac ggcgccgccg tagtatttac
    37501 ccgcctgcgg atcgttgata acaacaacca gcgcgaagcg cggctgactc gcaggcgcaa
    37561 cgcctgcggt ataagcaata tatttattga tgtagcgacc gtccggcccg acctttttcg
    37621 cggtaccggt tttaatggcg atacgatagc ctttaatcgc cgccttcacg ccgccgccgc
    37681 ctggtagcgc cacgctttcc atcatatgca ccacagtgcg gacaatggat tccgggaaga
    37741 cacgttcacc gggaaccggg gggtcaactt tggtaatcga cagtgggcga taaatgccgt
    37801 agctgccgat agttgcgtag actcgcgcta actgtaacgg tgttaccatt agcccgtagc
    37861 cgaaagagaa ggtggccctc tctatgtcag accaccgttg tttttgagga tataagccac
    37921 tgcgttctcc gaccaacccc aaattggtcg cttttcccag tccaaagcgt gagtaagtat
    37981 ctactaacgc tgaggacggc atcgctaacg ccagcttgga aacaccgacg ttactcgact
    38041 tctgtaatac cccggtcagg gttaattcgc tgtagcgtgc cacgtctttg atttcgtggc
    38101 cgttaattcg ataaggaatg gtattgagta ccgagttttc ccgcaccacg ccacgttgca
    38161 acgcggtcat taccaccatc ggtttaaccg ttgagcccgg ttcaaacacg tcggtgatgg
    38221 tacggttacg catcgcctct ttcggcgtgc cgctcagatt gttagggttg tatgacgggc
    38281 tgttagccat cgccagcact tcaccggtgt tgacatccac cagcacggcg ctaccagatt
    38341 cagccttgtt aaaggccacc gcgttgttca gttcgcgata aaccagcgcc tgcaggcgtt
    38401 catcaatact cagcgccagg ttgtgcgctg cctggctgtc agtagaagaa atatcttcaa
    38461 ttacgcgacc atagcggtct ttacgcacaa tgcgctcacc cggctgcccg gtaagccatt
    38521 tatcgaaact cttctcaacg ccctcaatcc cttgactatc gacgttagta aagccgatga
    38581 ggtgagcagt cacttcgccg gacggatagt aacggcgaga ctcttcacgc agatgaatcc
    38641 ccggcagttt cagttttttg atgtagtccg ccatgtcagg gttcacctga cgcgccagat
    38701 aaataaagcg ccctttcggg ttggcgttaa tgcgggctga aagctgatcc agcggaatat
    38761 tgagcgcgtt agccagcgcc ttccagcggt caccgacgct gataccgcca gcgtcatgca
    38821 cttctttcgg gtcagcccaa atcgctttta ccggcacgct caccgctaac gggcgaccag
    38881 aacggtcagt aatcatgccg cgggaggtgg aaacttgctg aacgcgaaga gaacgcatgt
    38941 cgccctcttt caccagcata tccggggaga taacttgtaa ccacgctacg cgtccgagca
    39001 gaaaagccag cgccaggaga atacagccgc ataacaacgc aaaacgccaa ctgataaagt
    39061 tggcatgttc ttcctgacgt tttggtttct gcgttttcgc cgctgctttc atgcgtcgcg
    39121 tttatcctta tttttgcact acgatatttt cttgtgacgg atcaacatgc tgcatttgca
    39181 gcttttccgt ggcgatcctt tccacccggc tatggtcgcc gagcgcattc tcttcaagga
    39241 tcaggttgcg ccattcaatg tctaaagcat ctcgctccag caccagttgt tcgcgctgag
    39301 cggtcagtaa acgggtatgg tgcgccgtgg ttaccacagt caccgccgtc aaaataatgc
    39361 aaatgaacag gcagagtggc agcttcccaa atcgcaaaag atcgtcaccg ataacaccag
    39421 gcaatgcatg gcgctcgtgg cttcccatcg atcctttaac tttgcttaga gcttctgtca
    39481 ctctgctgat catgcattcg tcctctctgc aatacgcaga actgaactac gggcacgagg
    39541 gttctcagcc acctcttctt cgcccggcat taacttgcct agtgctcgca gctgacggcc
    39601 acccagtttt ttgagctgct cttcagtcat cggtaaccct gccggaactt gcggaccgcg
    39661 gctgttttca cgcataaaac gtttcacaat acggtcttcc agcgagtgga agctgatgat
    39721 cgaaagccgc ccacccgggg ccagcacgtt gagcgagctt tttagcgcct gctctatctc
    39781 ctccagttca ctgtttaccc aaatgcgcac cgcctggaag gtacgggtcg cgggatgttt
    39841 aaacttatct ttcaccggcg ttgcagcagc cacgacttcc gccagttctt tggtgcgggt
    39901 catcggctgt tcgcggttac gctcgacaat ggcgcgggca atgcgtttgg caaaacgctc
    39961 ttcaccatag gttttcaata cccaggcgat atcggcttct tctgcggttt gtagccattc
    40021 agcggctgac tgcccacggg ttgggtccat acgcatgtcc agcggaccat cgcgcataaa
    40081 ggaaaagccg cgctcagcat catcaagttg cggtgaagag acgccaagat cgaggagaat
    40141 gccgtcgatc ttgccgataa gatcgcgctc ggcaacgtat tcgcccagcg cggagaaagg
    40201 tccgtggatg atggagaagc gcggatcatc aatagtcttc gcaacggcga tagcctgcgg
    40261 gtcgcgatcg atcgccagca aacgcccctc ttcgccaagc tgcgagagga tcagacgtga
    40321 gtgaccaccg cgaccaaaag tcccatcaat gtagatgcca tcaggacgga tattgaggcc
    40381 attaacggct tcatccagca gcaccgtagt atgtttatag ttttccatca ttttatagag
    40441 acaagtcctg cagtcgctcc gataagtctc cggtagccaa ctgctctgcg tcgatatctt
    40501 ccttgacctg ttgatgccag gttgtttcat cccacagctc aaacttgttg aactgtccaa
    40561 ccagcatcac ttcttttgtc agcccggcat gttgccgcag tactggcgcg attaacaatc
    40621 gacctgcgcc atccatctga cattcgctgg catgacctaa cagtaggcgc tgcacacggc
    40681 gctcaaccgg gttcatgctc gacagacgcg ataatttttg ctcgataatt tcccattcag
    40741 gcagggggta aagcagcagg cacgggtgat gaatgtcaat ggtgcaaacc atttgaccgg
    40801 cagcgttctc aagcagctgt tcccggtaac gggtaggcac tgataagcgc cctttgctgt
    40861 cgagattgac taacgttgct ccccggaaca tgccagcctc accccttatt cccactttgc
    40921 cccacaattt cccactgaaa ggagtttacg gagcggagga aaagcttgtc aagccagtca
    40981 caaggcatac cgaggtaaaa aacccgatgt ttacggctaa tatcagcgtt gtttatttta
    41041 cgtccaacgt gcgcagcaaa ttaacgttat gaatagctgt aagaaaaaat ccctcatcgt
    41101 catcaccgtt ttaaaatgac tcactatttt ccacagcaaa tataaagtgt cagtttgcga
    41161 cgcgagcggc attttaggac atatcttccc cggttaacag tccctgttgc gtctgtgtgg
    41221 cgcttgatgc cagcgggttt gcgcaaaagc aaagaaaagt gcttgttaaa tcggcaattc
    41281 atctctgcat gtaacaaaat aatgcaaaaa cgcgctgtcg atatcactta attattgtga
    41341 atttcagctg gtgagttaat taacgggcgg gtttaaaaga acttatcaat aatagtcagg
    41401 aatagtctta tttactttaa ggatattgat gtccattccc gtactctacg cgccagaggg
    41461 aaattcacct ggcgcgtatt tttgttcgcg gcttagctac ggctgagcac accgcggcga
    41521 tagagattac gtttaatgcg cgttaaacca ggttttggct tacgcggttc gtccaggctt
    41581 gccaggacaa tctccagcac acgctctgcg acatcgcggt gacgttgagc cactgccagc
    41641 accgggcact gtaagaagtc gagcagttcg ttatcgccaa aggtggcaat tgccaggtca
    41701 gaaggcagtt taccatcgcg acgtagcgtg acatccatca ccccttgcaa caacgcaaac
    41761 gacgttgtga acagcgcctg cggcatcgga tgcgtttcca gccatttttc gaataactgg
    41821 gcagccgcct cccgctcata gctgttggca tacaggaaat gcacttcgcg cggatcatct
    41881 ttccaggcag tacggaaacc ttgttcacgc aggaagctga cagaaagctc cggcagcgcg
    41941 ccaagataaa gcaccgtctc ggcgggaaac ttacgtaact cttccgccag catttcggca
    42001 tcatcctgat cggcaccaac cacgctggtg aaatgttcac gatcgagggc gcggtccagc
    42061 gcgacaatcg ggaacgggtc gttagcccag cgttgataaa aaggatgctc aggtggcaac
    42121 gacgtcgaaa caataatggc atcaacctga cgctgtaaaa ggtgctcaat gcagcgcatt
    42181 tcgttgtctg gctgatcttc tgagcaggca atcagcagtt gataaccccg ttgccgcgcc
    42241 tggcgctcaa gatagttagc gatgcgggta tagctggtgt tctccagatc ggggatcaca
    42301 agaccaatag aacgtgtgcg tccagcacga agcccagctg ccacggcgtt cgggtggtaa
    42361 ttgtgctcac gcaccacagc catgactttt tcaacggttt tgtcgctcac acggtattgc
    42421 ttcgctttgc cgttaataac atagcttgca gtggtccgcg acactcccgc cagccgagcg
    42481 atttcatcca gtttcacaat tgccccttgc gtaaaatgta aaaaccatag ccattgtact
    42541 ggtatgggtt aaattcatta acatctaagc gcagaaaatt aactgcggca actgctttta
    42601 ttccaggttc ccactgattt cgcaaaaaaa gcccaacgtg acatgttggg ctgcaaattg
    42661 cgcactgaga tcattctcaa cgcattattt tatcgccgcg cgaaagtccg accacaccag
    42721 agcgagcaac ttccacaatt ttcgccacat cgcgaatcga tgctaaaaat gcatcaagct
    42781 tatcgctggt gcctgctaat tgaacggtat aaagcgaggg ggtgacatcg ataatttgcc
    42841 cacggaatat ttccgtatta cgtttcactt cgtcacgccc gtaaccgctg gcctgaattt
    42901 tcaccagcat gatttcccgc tcaacatgcg cgccctgccc caactcactc acgcgcaaga
    42961 catcgaccag cttgtgtaat tgcttttcga tctgctcaag cactttttca tcgcccacgg
    43021 tctggatggt catacgcgat aatgtcggat cgtcggttgg cgcaacggtc aggctttcaa
    43081 tgttgtagcc acgctgggaa aaaaggccaa tcacgcggga taacgcgcct gattcatttt
    43141 cgagtaagac tgataatatc cggcgcataa tcaggttctc tccgttttgc ttaaccacat
    43201 ttcatccatt ccgcccccgc gaatctgcat cgggtagacg tgctcactgc catcgacggt
    43261 aacatcaaca aacaccaggc gattattgca cacctgttcc agcgcctcgc taagtttgct
    43321 ttccagctca tgcggatgag agatctggat cccgacgtga ccataggctt ccgccagacg
    43381 gacgaaatcg ggtagtgatt gcatataaga ttgtgaatga cggccggaat agatcatgtc
    43441 ctgccactgt ttcaccatcc ccagatagcg gttattgaga ttcaccacca gtaccggcaa
    43501 ctcgtattgc aacgcggtag acagttcctg gatgttcatc tgaatactgc cgtcgccagt
    43561 gacgcaaacc acggtttctt ctggcaacgc cattttgacg cccagtgccg caggtaaacc
    43621 aaaacccatc gtgccgaggc caccggaatt gatccagcga cgcggtttgt cgaatggata
    43681 ataaagtgca gcaaacatct ggtgctgccc gacatcggac gtcacgtaag cgtctccctt
    43741 cgtcaaccgc caaagagtct cgatcaccgc ctgcggttta atcttttcac tgtgagtgtc
    43801 atatttcagg cactgacgag cgcgccactg ttcaatttgc tgccaccagt cgcggatctc
    43861 atccagtggt tgatgggcgg attcttgcga caagagttca agcatttgtt cgaggacctg
    43921 gcgagcatcc cccacaatcg ggatatccgc agtcacggtt ttagaaatgg aagtaggatc
    43981 aatatcgata tgcagaacag tggcatttgg gcagtacttt gccagattgt tcgtcgttcg
    44041 gtcatcaaat cgtaccccga cggcgaaaat cacatccgcg ttatgcatcg tcatattggc
    44101 ttcgtaggta ccgtgcattc ccagcatgcc cagtgcctga cgatgcgttg ccggaaacgc
    44161 ccccagcccc atcaatgagg aaacaacggg cagattcaac gcctccaccg tttctttcaa
    44221 ctgctgatgg cagcccgcca tgattgcccc accgcctacg tagacaaccg gtttttttgc
    44281 cgctaccagc gtttgcagag cgcgcttaat ttgcccttta tgtccggtag tagtgggatt
    44341 gtaagaacgc atactgaccg actcaggcca gacatagggt aatttgttcg ccggattaag
    44401 aatatctttc ggtaaatcaa cgaccactgg tcctgggcga ccacttgccg ccagccagaa
    44461 agcctttttc agcacctgcg gaatgtcttc cgtttgctta accagaaaac tgtgtttaac
    44521 caccggtcgc gaaatcccca ccatgtcgca ctcctgaaag gcatcgtaac ctatcaacga
    44581 ggtcgctacc tgcccggaaa ggacaactaa tggaatggaa tccatataag cggtggcgat
    44641 gccggtaatc gcattggtcg cccctggacc cgacgttacc agcacgacgc cgacttcccc
    44701 ggtcgcgcgc gccagaccat cggccatatg cactgccgcc tgctcatgac gaactaaaac
    44761 atgatcgata ccacccacgg tatgcaatgc atcgtaaata tcaaggactg cgcctccggg
    44821 ataaccgaat acttgtttaa cgccctgatc gataagcgat cggacgacca tctcggctcc
    44881 agacaacatc tccatggcct gcctcactgt ttgacggaaa aaatgtgtaa aacacattaa
    44941 ccgtccgatc ttgcttaagc aattggcagc cagagacata aacgcagggt aataagaata
    45001 aacaggagga aaggtgaaaa gaaacgataa aatcctccat tacgaaggat taaattgata
    45061 ataaatcggc tgaatcccac aacttacagc aatttctcta tttttttaat taatcctaaa
    45121 atagagagcc cacacattca gaataaaatt ctacattcaa taaaaaatac agaataaacc
    45181 agacattcat gtctgaccta ttctgcattc agttagcgtt tgcaaattga gactaattgc
    45241 tcttccatcc actgatggcc tttatcgcgc ccggcagctt catgccagga gagataacag
    45301 gttctgctat tttgttttaa cggcagcggt aatacctgta attctaagga ttcagcgaac
    45361 tcttcagcca gccaacgcgg cgcaatagcg accaaatgcg tttgcgacac cacgctaagt
    45421 acgctcatca ttgccatgcc ctgatacgcg atactggctt gcttatctac cgtgtcatac
    45481 caaggttgac taaatgacgc gaaacgatcg agcgaaaccg ccgcatgttg ttcgttataa
    45541 acatcatgtt tcagtaacgg gcccttaatt gttggatgat ttttgctggc taccagcacc
    45601 atttcatctt taaataatgg cacgctggta aattcaggac gatggaagtc ttcataacta
    45661 atcacaaact ccgtttcctg ataacgcagc tgatgttcag tgttctgatt taatgaagac
    45721 ttgaacataa catgtatatt tggcgcaatc tgctcaatgt gattataaat ctgcgaggtc
    45781 agaatgctgt ctaacgggct gcaaacacaa agatgaaata cacgttcact gctcgcgggt
    45841 tcaaaacctg aaccaggcaa ttcattttgt actagttgca atgcctgacg aactgagcca
    45901 aaaagttgaa atgcgcgagc agtcggttga ataccacggc cataacgaac aaaaagctcg
    45961 tcattaaaca tcaccttcag gcgtgcaaca gcgttactga ccgcaggttg cgacataccc
    46021 agaacatgag cggcacgggt aatgttttgc tcctgcatca cggcatcgaa aacggttaat
    46081 aagttgagat cgaccatgcg tagctgtggc ttgcttaact ccgccgtctc tgaatgatct
    46141 gtttgtacct ctggcatact taactccact gtcacactta actccctttc ccttattgga
    46201 atgcagagaa ataatcctga agaatatgat ttatcatgca tataaaataa gaaaaagcaa
    46261 aatgagtaaa attcggaaaa cataaagata cgggttctga taaaaacaga atccattgcc
    46321 gtggaatgag tcatttacca cagcataata atccataatg tttttgttgc gaaaacaatc
    46381 taatcataac taccgcgaat actcaatcat ctacaaaatg gattaaatgt gcttatttaa
    46441 taattaattt atatatttaa tgcattaatt cttaacatta attgatcaat aatattcacc
    46501 aaatcaatat caaaaaaaat cgcaaaacat ataattcaat acaaatcatc aggataggtt
    46561 ttgcaacgcg tgcattttgt cccctttttc ctcgttgatt agatgcaaaa atttatgctg
    46621 aaatatgtca accgatgaaa agcgtcggta gttaagcaga aattaatatc gcttacttta
    46681 accaccgcag cacaattagc taattttacg ggtgcagaac tcacgctggc gggacgtttt
    46741 tattgcgtca gggttgacat ccgtttttgt atccagtaac tctaaaagca tatcgcattc
    46801 atctggagct gatttaatga ctcacatcgt tcgctttatc ggtctactac tactaaacgc
    46861 atcttctttg cgcggtagac gagtgagcgg catccagcat taagccagca cgcagtcaaa
    46921 caaaaaaccc gcgccattgc gcgggttttt ttatgcccga agcgaggcgc tctaaaagag
    46981 acaaggaccc aaaccatgag ccagcaagtc attattttcg ataccacatt gcgcgacggt
    47041 gaacaggcgt tacaggcaag cttgagtgtg aaagaaaaac tgcaaattgc gctggccctt
    47101 gagcgtatgg gtgttgacgt gatggaagtc ggtttccccg tctcttcgcc gggcgatttt
    47161 gaatcggtgc aaaccatcgc ccgccaggtt aaaaacagcc gcgtatgtgc gttagctcgc
    47221 tgcgtggaaa aagatatcga cgtggcggcc gaatccctga aagtcgccga agccttccgt
    47281 attcatacct ttattgctac ctccccaatg cacatcgcca ccaagctgcg cagcaccctg
    47341 gacgaggtga tcgaacgcgc tatctatatg gtgaaacgcg cccgtaatta caccgatgat
    47401 gttgaatttt cttgcgaaga tgccgggcgt acacccattg ccgatctggc gcgagtggtc
    47461 gaagcggcga ttaatgccgg tgccaccacc atcaacattc cggacaccgt gggctacacc
    47521 atgccgtttg agttcgccgg aatcatcagc ggcctgtatg aacgcgtacc taacatcgac
    47581 aaagccatta tttccgtaca tacccacgac gatttaggcc tggcggtcgg caactcactg
    47641 gcggcggtac atgccggtgc acgccaggtg gaaggtgcaa tgaacgggat cggcgagcgt
    47701 gccggtaact gttcgctgga agaagtcatc atggcgatta aagttcgtaa ggatattctc
    47761 aacgtccaca ccgccattaa tcaccaggag atatggcgca ccagccagtt agttagccag
    47821 atttgtaata tgccgatccc ggcaaacaaa gccattgttg gcagcggcgc attcgctcac
    47881 tcctccggta tccaccagga tggcgtgctg aaaaaccgcg aaaactacga aatcatgaca
    47941 ccagaatcta ttggtctgaa ccaaatccag ctgaatctga cctctcgttc ggggcgtgcg
    48001 gcggtgaaac atcgcatgga tgagatgggg tataaagaaa gtgaatataa tttagacaat
    48061 ttgtacgatg ctttcctgaa gctggcggac aaaaaaggtc aggtgtttga ttacgatctg
    48121 gaggcgctgg ccttcatcgg taagcagcaa gaagagccgg agcatttccg tctggattac
    48181 ttcagcgtgc agtctggctc taacgatatc gccaccgccg ccgtcaaact ggcctgtggc
    48241 gaagaagtca aagcagaagc cgccaacggt aacggtccgg tcgatgccgt ctatcaggca
    48301 attaaccgca tcactgacta taacgtcgaa ctggtgaaat acagcctgac cgccaaaggt
    48361 cacggtaaag atgcgctggg tcaggtggat attgtcgcca actacaacgg tcgccgcttc
    48421 cacggcgtcg gcctggccac cgatattgtc gagtcctccg ccaaagccat ggtgcacgta
    48481 ctgaacaata tctggcgtgc cgcagaagtc gaaaaagagt tgcaacgcaa agctcaacac
    48541 aacgaaaaca acaaggaaac cgtgtgatgt cgaagaatta ccatattgcc gtattgccgg
    48601 gggacggtat tggtccggaa gtgatgaccc aggcgctgaa agtgctggat gccgtgcgca
    48661 accgctttgc gatgcgcatc accaccagcc attacgatgt aggcggcgca gccattgata
    48721 accacgggca accactgccg cctgcgacgg ttgaaggttg tgagcaagcc gatgccgtgc
    48781 tgtttggctc ggtaggcggt ccgaaatggg aacatttacc accagaccag caaccagaac
    48841 gcggcgcgct gttgcctttg cgtaagcact tcaaattatt cagcaacctg cgcccggcaa
    48901 aactgtatca ggggctggaa gcattctgtc cgctgcgtgc agacattgcc gctaacggct
    48961 tcgacatcct gtgcgtgcgc gaactgaccg gcggcatcta tttcggtcag ccaaaaggcc
    49021 gcgaaggtag cggacaatat gaaaaagcgt ttgataccga ggtgtatcac cgttttgaga
    49081 tcgagcgtat tgcccgcatc gcgtttgaat ctgctcgtaa acgtcgccac aaagtcacct
    49141 caatcgacaa agctaacgtg ctgcaatcct ctattttatg gcgtgaaatc gtcaacgaga
    49201 tcgccacgga atacccggat gtcgaactgg cgcatatgta catcgacaac gccaccatgc
    49261 agctgattaa agatccatca cagtttgacg ttctgctgtg ctccaacctg tttggcgaca
    49321 ttctgtctga cgagtgcgca atgatcactg gctcgatggg gatgttgcct tccgccagcc
    49381 tgaacgagca aggttttggt ctgtatgaac cggcaggcgg ctcagcacca gatatcgcag
    49441 gcaaaaacat cgccaacccg attgcgcaaa ttctatcgct ggcactgctg ctgcgctaca
    49501 gcctggatgc cgatgatgcg gcttgcgcca ttgaacgcgc cattaaccgc gcattagaag
    49561 aaggcattcg caccggggat ttagcccgtg gcgctgccgc cgttagtacc gatgaaatgg
    49621 gcgatatcat tgcccgctat gtggcagaag gggtgtaatc atggctaaaa cgttatacga
    49681 aaaattattc gacgctcacg tagtgtacga agccgaaaac gaaaccccgc tgttatatat
    49741 cgaccgccac ctggtgcatg aagtgacctc accgcaggcg ttcgatggtc tgcgcgccca
    49801 cggtcgcccg gtacgtcagc cgggcaaaac cttcgccact atggatcaca acgtctctac
    49861 ccagacgaaa gacattaatg cctgtggtga aatggcgcgc atccagatgc aggagctgat
    49921 taaaaactgc aaagaatttg gcgtcgaact gtatgacctg aatcacccgt atcaggggat
    49981 cgtccacgta atggggccgg aacagggcgt caccttgccg gggatgacca ttgtctgcgg
    50041 cgactcgcat accgccaccc acggcgcgtt tggcgcactg gcctttggta tcggcacttc
    50101 cgaagttgaa cacgtactgg caacgcaaac cctgaaacag ggccgcgcaa aaaccatgaa
    50161 aattgaagtc cagggcaaag ccgcgccggg cattaccgca aaagatatcg tgctggcaat
    50221 tatcggtaaa accggtagcg caggcggcac cgggcatgtg gtggagtttt gcggcgaagc
    50281 aatccgtgat ttaagcatgg aaggtcgtat gaccctgtgc aatatggcaa tcgaaatggg
    50341 cgcaaaagcc ggtctggttg caccggacga aaccaccttt aactatgtca aaggccgttt
    50401 gcatgcgccg aaaggcaaag atttcgacga cgccgttgcc tactggaaaa ccctgcaaac
    50461 cgacgaaggc gcaactttcg ataccgttgt cactctgcaa gcagaagaaa tttcaccgca
    50521 ggtcacctgg ggcaccaatc caggccaggt gatttccgtg aacgacaata ttcccgatcc
    50581 ggcttcgttt gccgatccgg ttgaacgcgc gtcggcagaa aaagcgctgg cctatatggg
    50641 gctgaaaccg ggcattccgc tgaccgaagt ggctatcgac aaagtgttta tcggttcctg
    50701 taccaactcg cgcattgaag atttacgcgc ggcagcggag atcgccaaag ggcgaaaagt
    50761 cgcgccaggc gtgcaggcac tggtggttcc cggctctggc ccggtaaaag cccaggcgga
    50821 agcggaaggt ctggataaaa tctttattga agccggtttt gaatggcgct tgcctggctg
    50881 ctcaatgtgt ctggcgatga acaacgaccg tctgaatccg ggcgaacgtt gtgcatccac
    50941 cagcaaccgt aactttgaag gccgccaggg gcgcggcggg cgcacgcatc tggtcagccc
    51001 ggcaatggct gccgctgctg ctgtgaccgg acatttcgcc gacattcgca acattaaata
    51061 aggagcacac catggcagag aaatttatca aacacacagg cctggtggtt ccgctggatg
    51121 ccgccaatgt cgataccgat gcaatcatcc cgaaacagtt tttgcagaaa gtgacccgta
    51181 cgggttttgg cgcgcatctg tttaacgact ggcgttttct cgatgaaaaa ggccaacagc
    51241 caaacccgga cttcgtgctg aacttcccgc agtatcaggg cgcttccatt ttgctggcac
    51301 gagaaaactt cggctgtggc tcctcgcgtg agcacgcacc gtgggcattg accgactacg
    51361 gttttaaagt ggtgattgcg ccgagttttg ctgacatctt ctacggcaat agctttaaca
    51421 accagctgct gccggtgaaa ttaagcgatg cagaagtgga cgaactgttt gcgctggtga
    51481 aagccaatcc ggggatccat ttcgacgtgg atctggaagc gcaagaggtg aaagcgggag
    51541 agaaaaccta tcgctttacc atcgatgcct tccgccgcca ctgcatgatg aacggtctgg
    51601 acagtattgg gcttaccttg cagcacgacg acgccattgc cgcttatgaa
//
LOCUS       DAMZET010000012        47341 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.012, whole genome shotgun sequence.
ACCESSION   DAMZET010000012 DAMZET010000000
VERSION     DAMZET010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.012"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            154..1251
                     /locus_tag="POB34_000845"
     CDS             154..1251
                     /locus_tag="POB34_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917838.1"
                     /translation="MLHAQLIDVYNAEADSHNSLVDAAENMQLDLGLKGKEIEELQLQ
                     IEHLKRQQQDAIDDATHDANQRADNAERISIELETKLNEMTAMVELRNSQISTLKSQY
                     KEIMKLDPFNLEKRYNKAKSERQELRKQVADLNQQLKKTIKDASEARVAFANKKAEVT
                     ALVNENAKFATLKKEMYGITERRFPASKLHPTLGQISFFPRLLAYGISSPKEFNNERP
                     YIVSKLDFAYQFCCDMGYAIDIRINEWLMPNFQPLAIFREFQPEGWVEFFHELICKEM
                     ESRRPELVRRVEWAQEVMLADAELPFEPEFIDDLATKGLHTLFDVVTRRHEQLVVELG
                     LEETAARRLLDVCYARSDAWEKENGGTIYVR"
     gene            1324..1830
                     /locus_tag="POB34_000846"
     CDS             1324..1830
                     /locus_tag="POB34_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-phosphatase"
                     /protein_id="HDI5917839.1"
                     /translation="MSIFNKHAHQERPYIVIVDIDGTISEATEDRLHLLPPPGKGALT
                     KDWNEFNLACDTDTPITPVIDMVRQLFNVYTVWFVTGRCEIARDKTRAWLRKYVTNGA
                     EPLLSMRPATDDRNDGPAKIDLLKKIGLSKIAFALEDKIEVARVFRRHGVLTLMVREY
                     ENALLHQQ"
     gene            complement(2095..5211)
                     /locus_tag="POB34_000847"
     CDS             complement(2095..5211)
                     /locus_tag="POB34_000847"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HDI5917840.1"
                     /translation="MTHQTHTIAESNNFIVLDKYIKAEQTSDSYQSESDLERELIQDL
                     RNQGYEFISVKSQSAMLSNVREQLQSLNGVMFNDSEWRRFTEQYLDNPSDGILDKTRK
                     IHIDYICDFIFDDGRLENIYLIDKKNLMRNKVQIIQQFEQTGSHANRYDVTILVNGLP
                     LVQIELKKRGVAIREAFNQIHRYSKESFNSENSLFKYLQLFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWAKSDNTLIKDLKDFTATFFQKHTLLNVLVNYSVFDSSQTLLVMRPYQ
                     IAATERILWKIKSSFTAKNWSKPESGGYIWHTTGSGKTLTSFKAARLATELDFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSENTAGLKRNLDKDDNKIIVTTIQKLNNLMKA
                     ESDLPVYNQQVVFIFDECHRSQFGEAQKNLKKKFKRYYQFGFTGTPIFPENALGSETT
                     ASVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKSLETETDEKKLSAAENQQAFL
                     HPMRIKEITQYILNNFRQKTHRTFPGSKGFNAMLAVSSVDAAKTYYATFKRLQEEAAN
                     KSATYKPLRIATIFSFAANEEQNAIGEICDETFDTSAMDSSAKEFLDAAIREYNSYFK
                     TNFSTDGNGFQNYYRDLAQRVKNQDIDLLIVVGMFLTGFDAPTLNTLFVDKNLRFHGL
                     MQAFSRTNRIYDATKTFGNIVTFRDLERSTIDAITLFGDKNTKNVVLEKSYAEYMEGF
                     TDATTGEAKRGFMTVVSELEQRFPDPTSIESEKEKKDFVKLFGEYLRAENILQNYDEF
                     ATLKALQQIDLSDPVAVEKFKAEHYVDDEKFAELQTIRLPADRKIQDYRSAYNDIRDW
                     QRREKEAEKKEKSTTDWDDVVFEVDLLKSQEINLDYILGLIFEHNRQNKGKGEMIEEV
                     KRLIRSSLGNRAKEGLVVDFIQQTNLDDLPDKASIIDAFFTFAQREQQREAEALIKEE
                     NLNEDAAKRYIRTSLKREYATENGTELNETLPKLSPLNPQYKTKKQAVFQKIVSFIEK
                     FKGVGGKI"
     gene            complement(5333..6526)
                     /locus_tag="POB34_000848"
     CDS             complement(5333..6526)
                     /locus_tag="POB34_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDI5917841.1"
                     /translation="MSGLNFLNKLLDGVDVEWLSLGELGDFIRGNGLQKKDFVESGFP
                     AIHYGQIYTRYGLSADKTFTYVLPELANKLRKAQKNDLLLATTSENDEDVVKPLAWLG
                     DNVAISGDMMLFRHEQNVKYLAYFFQSEIFQTQKMKYITGAKVRRVSSGDLSKIKIPI
                     PSSNNPEKSLTIQSEIVRILDKFTALTAELTAELNMRKKQYNYYRDQLLSFKEGEVEW
                     KTLGEVAVIGTGNHDTQDAIEHGKYIFYARGREPLKLNVFDFDETAIITAGDGAGVGK
                     VFHYAKGKYALHQRAYRIVPNAFMNPRFVYHYITAYFFTYIQKASVSSSVTSLRRPMF
                     LKFPIPVPPSEEQARIVEILDKFDTLTNSITEGLPREIELRQKQYEYYRDLLFSFPKP
                     ETVSN"
     gene            complement(6523..8079)
                     /locus_tag="POB34_000849"
     CDS             complement(6523..8079)
                     /locus_tag="POB34_000849"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDI5917842.1"
                     /translation="MTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSSYIEAGDDSICYAKLDDSVITDDIKDDAIKTKGYFIYPSQLFCNVAAKANTNDR
                     LNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNARLAAVLKGVE
                     GLKLGDFNEHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTSV
                     NKIYDPAAGSGSLLLQAKKQFDNHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFD
                     IKLGNTLTEPHFRDEKPFDAIVSNPPYSVKWIGSDDPTLINDERFAPAGVLAPKSKAD
                     FAFVLHALNYLSAKGRAAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVISLAPNLFFG
                     TTIAVNILVLSKHKTDTNVQFIDASELFKKETNNNILTDAHIEQIMQVFASKEDVAHL
                     AKSVAFETVVANDYNLSVSSYVEAKDTREIIDIAELNAELKSTVGKIEQLRKDIDSIV
                     AKIEGYEAQS"
     gene            8262..8483
                     /locus_tag="POB34_000850"
     CDS             8262..8483
                     /locus_tag="POB34_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDI5917843.1"
                     /translation="MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATF
                     EAYKKAALDAEFASLFDTLDSTNKELVNR"
     gene            8483..8863
                     /locus_tag="POB34_000851"
     CDS             8483..8863
                     /locus_tag="POB34_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDI5917844.1"
                     /translation="MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVA
                     YEEITDLFEVSATYLVATARGHIFNDANKRTALNSALLFLRRNGVQVFDSPELADLTV
                     GAATGEISVSSVADTLRRLYGSAE"
     gene            8868..9047
                     /locus_tag="POB34_000852"
     CDS             8868..9047
                     /locus_tag="POB34_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PdcA protein"
                     /protein_id="HDI5917845.1"
                     /translation="MARKYNKLSREALKMLLDGVSRREVKQYLVGKQVGVRTAIAVLC
                     RQEMVVLKQRMPGSR"
     gene            9075..10118
                     /locus_tag="POB34_000853"
     CDS             9075..10118
                     /locus_tag="POB34_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDI5917846.1"
                     /translation="MKAIITPFVQKELGVATFKVDQEVRKLVEAGRKFIMEPVPRELI
                     EHMDDGLVVSEQTMATNEALQPFFNSDELFRRIGGIDALVAWLRRKEGRCQAADRSWC
                     DNHIVHAERDNSAVLLCWHHDNHYRMRGFNELKETLHNNRVNWILDVARQEMGLSDGH
                     DLSIQELCWWAFMRDMMHLMPEEVCRISINKIKAATQDSGPMKEADIRPYDDRATAYV
                     QMMEERAAPMRAKVCPVDVDSDPGMAHFKIPKLQSLKLPEYMDFVASRPCCGCGAAGA
                     GAHITPYIVRHSRLCAHDIYAIPLCQSCQRDIERDRDNWEKTHGRLAMHQRLFFDYAL
                     GVGAITSHSSSVR"
     gene            10207..10659
                     /locus_tag="POB34_000854"
     CDS             10207..10659
                     /locus_tag="POB34_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Late promoter-activating protein"
                     /protein_id="HDI5917847.1"
                     /translation="MLLNWQGRHFMEINHSRITSYEIADYMIRTKSLLSAKELAAILE
                     KEYPHLDVDKRDVYLRLKAIAVSKYSSVLIDDSTRPRRFQIHSLNPEFFRRSRAPRRF
                     DEKLQNELYMTQDEKERREHQPWVMARQLFNKVARQHRHYGNATSARI"
     gene            10745..11938
                     /locus_tag="POB34_000855"
     CDS             10745..11938
                     /locus_tag="POB34_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDI5917848.1"
                     /translation="MTWDDHKKNFARLARDGGYTIAQYAAEFNLNPNTARRYLRAFKE
                     DTRTVDSRKPNKPVRKPLKSMIIDHSNDQHAGDHIAAEMAEKQRVNAVVSAAVENAKR
                     QNKRINDRSDDHDVITRAHRTLRDRLERDTLDDDGERFEFEAGDYLIDNVEARKAARA
                     MLRRSGADVLETTLLEKSLSHLLMLENARDTCIRLVQEMRDQQKDDDEGTPPEYRIAS
                     MLNSCSAQISSLINTIYSIRNNYRKESREAEKHALSMGQAGIVKLAYERKRENNWSVL
                     EAAEFIEAHGGKVPPLMLEQIKADLRAPKTNTDDEERQTAVGGPSLEDLDKVARERAA
                     NRRADAALWIEQRREEIADIVDTGGYGDVDTEGVSNDPWLEQDLDEDEEEDEEVTRKL
                     YGDDD"
     gene            11938..13422
                     /locus_tag="POB34_000856"
     CDS             11938..13422
                     /locus_tag="POB34_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDI5917849.1"
                     /translation="MARSCVTDPRWRELVALYRYDWIAAADVLFGKTPTWQQDEIIES
                     TQQDGSWTSVTSGHGTGKSDMTSIIAILFIMFFPGARVILVANKRQQVLDGIFKYIKS
                     NWATAVSRFPWLSKYFILTETSFFEVTGKGVWTILIKSCRPGNEEALAGEHADHLLYI
                     IDEASGVSDKAFSVITGALTGKDNRILLLSQPTRPSGYFYDSHHRLAIRPGNPDGLFT
                     AIILNSEESPLVDAKFIRAKLAEYGGRDNPMYMIKVRGEFPKSQDGFLLGRDEVERAT
                     RRKVKIAKGWGWVACVDVAGGTGRDKSVINIMMVSGQRNKRRVINYRMLEYTDVTETQ
                     LAAKIFAECNPERFPNITIAIDGDGLGKATADLMYEYYGITVQRIRWGKKMHSREDKS
                     LYFDKRAYANVQAAEAVKSGRMRLDKGNETIEEASKIPVGINSAGQWKVMSKEDMKKK
                     LNLHSPDHWDTYCFAMLADYVPQDEVLSVEDEAQVDEALAWLNE"
     gene            13506..13983
                     /locus_tag="POB34_000857"
                     /pseudo
     CDS             13506..13983
                     /locus_tag="POB34_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908421.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            13980..15098
                     /locus_tag="POB34_000858"
     CDS             13980..15098
                     /locus_tag="POB34_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDI5917850.1"
                     /translation="MRTSLVTREEMIEAIEQHTACISTRDIPGVIANYFMITKQLYRR
                     KDKNAVHRILLSDIREYLLEQGHLNYATVAAESRKEAHRMKATNVKSEKIHAPSVQES
                     ELVVVQNQSDEIPVLEWQGVRVVTTETLARGYGTETIRIRQNHHENKVRFVEGKHFFK
                     VEGESLRELKHRVALNYSVKIARNVRSLTLWTERGAARHAKMLETDQAWAFFEKLEDS
                     YFRQKEQQPVAIPQTLPEALRLAAELAEQKQLLEQKAHQLNQQLVAAAPKVDFADRVS
                     VAKGILIGNFAKVVGLKQNALFAWLRENGILIASGGRKNVPFQQYINAGYFTVKEVVL
                     DDEDGYQIRLTPQLTGKGQQWLTRKLLDAGLLKPVAAE"
     gene            complement(15131..15982)
                     /locus_tag="POB34_000859"
     CDS             complement(15131..15982)
                     /locus_tag="POB34_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917851.1"
                     /translation="MINYVYGEQLYQEFVSFRDLFLKKAVARAQHVDTASDGRPVRPV
                     VVLPFKETDSIQAEIDKWTLMARELEQYPDLNIPKTILYPVPNILRGVRKVTTYQTEA
                     VNSVNMTAGRIIHLIDKDIRIQKSAGFNEHSAKYIENLEATKELMKQYPEDEKFRMRV
                     HGFSETMLRVHYISSSPNYNDGKSVSYHVPLCGVFICDETLRDGIIINGEFEKAKFSL
                     YDSIEPIICDRWPQAKIYRLADIENVKKQIAITREEKKVKSAASVTRSRKTKKGQPVN
                     DNPESAQ"
     gene            complement(16093..16302)
                     /locus_tag="POB34_000860"
     CDS             complement(16093..16302)
                     /locus_tag="POB34_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c1 repressor inactivator"
                     /protein_id="HDI5917852.1"
                     /translation="MAFIQPTIDDVRHCSNALSVDPAETDAARAIAEHYSKISNQEYR
                     ITQDDLDDLTDTIEYLMATNQLDSQ"
     gene            16907..17128
                     /locus_tag="POB34_000861"
     CDS             16907..17128
                     /locus_tag="POB34_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase"
                     /protein_id="HDI5917853.1"
                     /translation="MGYSAAKVSTHLELEKNRGYWRAKGFDRDSCQLSLSRGEEKIER
                     TRGRWRFYDENHKQVKAEPILYTLLKTII"
     gene            17136..18167
                     /locus_tag="POB34_000862"
     CDS             17136..18167
                     /locus_tag="POB34_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDI5917854.1"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKM
                     LLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRS
                     GLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRN
                     LAFLGIAYNTLLRIAEISRIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVT
                     KLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAK
                     DDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGA
                     MVRLLEDGN"
     gene            18218..18529
                     /locus_tag="POB34_000863"
     CDS             18218..18529
                     /locus_tag="POB34_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysogeny establishment protein"
                     /protein_id="HDI5917855.1"
                     /translation="MRKDFNIDGKYVVLSVSTNILSPSVIVTVKLSDRMPDIDSISVA
                     FPVKSMRSAEHFVMNATEEEARRGLTRVMGEFGELLGKVNNALSISSARSKALTASMM
                     K"
     gene            18777..19337
                     /locus_tag="POB34_000864"
     CDS             18777..19337
                     /locus_tag="POB34_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ref family protein"
                     /protein_id="HDI5917856.1"
                     /translation="MKTIEQKLEQRREWQKAARERAIARQREKFADPAWRESQYQKMR
                     DSIDRRIAKQKERPPASKTRKSAVKIKSRGLKGRTPTAEERSIANALGALPCIACYMH
                     GVISEEVSLHHIAGRTAPGCHKKQLPLCRWHHQHAAPAEVREKYPWLVPVHADGVVGG
                     KKEFTLLNKSEMELLADAYEMANIMH"
     gene            19526..20167
                     /locus_tag="POB34_000865"
     CDS             19526..20167
                     /locus_tag="POB34_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maturation control protein"
                     /protein_id="HDI5917857.1"
                     /translation="MDKKICVVSMSVGKPASMTAAWINNELIMAERTSYPERRRDMEL
                     QLLRELREKEEKGFIVLVEEENSFITGRVGQRVRLRDPFMNGRPVLIEAMQIYKELER
                     QKAIKLPRKESGKYILHQSIFDSEHDKKGDEFFNINWSEITTEHVLTLLCCFATEYNN
                     VASADYIRAMAGEVEARQEPSLLSPLINIIRGTQTLAQKQVPKGVLTGKGNYL"
     gene            20224..21528
                     /locus_tag="POB34_000866"
     CDS             20224..21528
                     /locus_tag="POB34_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDI5917858.1"
                     /translation="MSEYIEIAYAAATHRLCFLTGTGFSKAVSDDKAPSWQSLLEQLC
                     GLLKDGDSLKEELFPDGKAKDLSLEEAAQVIALKFILSGKNIYQEIEKIIASIELDPS
                     IEYIQDFFKENTFKVITTNYDKLAEKLAGENRTCTITPGLPIPKYNCEVKVYHVHGSI
                     DSPSDMVVTSEDYFRFINGNSYFSNKLSTVLHENTIVILGYSLSDANLKAIINEYKVF
                     SRDNVMSSNIFLISRGKLLQPIKDYYFSCFGIRVIDKTEVSDFFRKLNKKIPEAKKIK
                     DKLRHSIKSVIKNGREYKIEFLKLEDSFYHIISSISSSGYSWNDEKVLNVFCDIIDKK
                     IDLTKESGAWEQYEHLAKWLIYFGSLFEVKGTNFEKKYIHAVEHSMTYMNKPYETGYS
                     WRAYLAWKTKWPSLTASNRSLIKSKMEEIPLQQIHDIISKFI"
     gene            complement(21570..21818)
                     /locus_tag="POB34_000867"
     CDS             complement(21570..21818)
                     /locus_tag="POB34_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="modulator protein"
                     /protein_id="HDI5917859.1"
                     /translation="MKKRYYTVKHGTLRALQEFADKHNVEVRREGGSKALRMYRPDGK
                     WRTVVDFKTNSVPQGVRDRAFEEWEQIIIDNALLLNAD"
     gene            complement(21815..22255)
                     /locus_tag="POB34_000868"
     CDS             complement(21815..22255)
                     /locus_tag="POB34_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDI5917860.1"
                     /translation="MATLSDTIKPNKTYLEAVLRTALLGKTEDEYVDFFLSGLRGRLL
                     KNPRLYRSYGPYWPEIKKLLLERGYGNFGRLVDRDVRKIYRYDRPALTLIAATLYSQE
                     RFDNGQIYSAWHLLPVPEEVDDQDYEFESYDLEVEALAQAGEKT"
     gene            complement(22289..29056)
                     /locus_tag="POB34_000869"
     CDS             complement(22289..29056)
                     /locus_tag="POB34_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDI5917861.1"
                     /translation="MNKLSMGVFRCSSVSEILKYIRAITSHRAPIKYGVEKVEGKSYD
                     RLRREANQKAIDLLNSLVDGATLTDEQRQILAGYTGEGGIGGSVSEYYTPKPIAEGVW
                     EIMKLYGADVGNTLEPSAGTGVFNETKPVGTVMTATEISSVSGRINQLLHPEDSVQIS
                     PFEQLAVSTPNDSFDHVVGNVPFGGRDNTRNIDKPYAEETDMGSYFMLRMLDKIKPGG
                     FMCVIVPPSIVSGSNMKRLRLRLSRKAEFLGAHRLPTGTFDANGTSTVVDVVLMRKHP
                     AEMAEKIPLVDESTLESANVLWPTFISGKWFEKDGRRFVHGTQEKGFQGRIEVRADGQ
                     IDNQALKAKLIHRFESRIDWSLLDMAEPSPTADVVGEGEMRLINGVWQKYAGGRWIEA
                     DAGKELKIDAASYGADSWEALQRNLTTTEGRLGMTFTQMANVRDKYTTSISDDMVQLV
                     DWINSQPEKYRERLYRGAMIGRMLIEYQDMKAAGHSAEQIEQQRLSLVSRLQAEIDRF
                     GNPGRGPIAKLSGSGARAWFAFRGAIKLDGTISDELTGKLVTHDSSASYDSTSYQDTL
                     RYLYSDLTRDPIQLDDFRLAFTGELPASDDELLNLLASTPGIAVSPYGGIVPFARATS
                     GDINEIVAPKQEFLATLPDGPVKNNVLNQLAAIEEKRIKTPAENIRFKLNSRWFDRSV
                     ILEFLQENGYPDLRYVQSVQLEGDEMVSDTYHGGDGLFVGHRYGVVQRKDKETGEIRY
                     EWDRKSGENATGFPAQLEKYLNGARIGGKDSATANGYREQMALLEDQFNKWIKTHDRY
                     DELVAKYNDVFNSNIPYEHSGDPLGLKGLSGKRQPFDYQNSEVRRLSEDGRGILGFGT
                     GLGKTTTALALEAFNYENGRSTRTAYVVPKSVLENWYYEAKEFLSEEAFSNYLFVGLD
                     VLMDGDQIRQVPVLDENGKPVVGADGTPVMRDALKLADEATITARMNAIPHSNYRAVV
                     FTKEQYARIPLRDDTVDEHAQDMLYDFVAAGRVASAMDSDSHRKEAARRRVLSEYSDT
                     GTEKAEKYPYFEDMGFDSVIADEGHNYRNSYKNGREASQLAYLPTSAVAQSARDMAIK
                     NAYLMKKNGGRGPVLLTATPVVNTPIDAYNMLSHVLPKEYWQKMGIYGPDDFVKFFGK
                     TRLETVQKISGEVEEKMALVGFENLDALRGIFHRWTTLKTAEDVKDTVEIPELDEHQQ
                     DAPLTEEQLAAYEELRQQAEAAAKANNGVTTSVNEDGVIEHEKARPIFSIIRDMDRVC
                     TDMDLYYRRITYRFLPEYADAVQQLADSLPKQATSEDDDSDDSITQQSQYSLIDKGEF
                     IQLQVPEAFEQEVNKRLAKFGIDEQTVTHPVTPKYVKLIATLKEFFPEGKQIIFTDEK
                     TQHQKLKRIICNALNLEPSKVGILNAQTVAEAGKTGKKLKAVKPPKELPDEPTDAQIA
                     KYNEQMALYDAYIAQQNEMSLGGLEKIAADFQEGRTPIIICNKKAEVGINLHRGTTDI
                     HHLTLPWTPASIAQRNGRGARVGSNRASVRVHYYCGKGSFDEYRLKTLKRKAGWISDI
                     LRSDKSEMENADANDMIEMQMYTAKDDGERLAMMQVQMDKAKAAQRARQKEQATIDLH
                     NYIKAQHAAGEDVEVLTAELERSKAELEKTTAEVAKFKQAVMAKAADNADWKARWGSV
                     HHTDRTLLAQYRASLKSAIQRKANISQAISRYEKLLNRTQKAATDIKRLRPLVEDAIN
                     KGILDVDPDLVNHASEFLVIGDRSWRVGQYYDCAGDIVRIKSLDFDSQRADVEIIFTF
                     KGTKSGNWDVKTLDKQVDVTPDEDAVMQKISGGVSIAGINDIISCDDFYRFQQRGMIK
                     ITDSYGVQTTESGYSIDFVGTYSDPLKHAVYPDRRDGALKSSIAKWVLGMMSEGNNRQ
                     IRSAETFLVELFGSNYGDVIASYGDTLSPEAIQEKIADAIARMPEKTSQGATRNGDSE
                     LEVTNAIFGTNEFRASDYEITTAQFGTIGIYSNKDEIKQSMDAASARIAAERKANLNH
                     AVSALTQSWVTAIREAATTGKITPAIADVVNDGSKFMDAYKMDAVQLPSAYGQLSYRM
                     TYNLVSMFSDLAILGLVDLNEVTPELLSMRKNHVEILQRINTVLAGRTDEEKQADADR
                     INLALGNITEEEIAARNEKQEELSSIQGDATSIAQSLGLNYRVSTADLKMMYAPKFAA
                     GEVFGLQEASGMKGVLFRAKDAIKAKFGARWLPAKAKNSDFPGNWWIIETKHNVADVL
                     AVIQQYA"
     gene            29132..30841
                     /locus_tag="POB34_000870"
     CDS             29132..30841
                     /locus_tag="POB34_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDI5917862.1"
                     /translation="MADNKITLSSVRKALAGVFKDNGERDNILLSALAVHGGSGYLFS
                     RAGAPVQLSGFLGGKPGDSGMAGDGLVDGSRFIFDEVQLPEDRLQRYPLLEEMAVYST
                     IATALNIHITHALSFDKKTGQTFSIVPVHNGNDSDYDAAQALCDELMNDIGRTINKEV
                     AGWAFIMSVFGVAYVRPYAKEGIGITSFECSYYTLPSFIKEFEVSGNLAGFSGDYLKD
                     ASGKMVFADPWAIIPMKIPYWRPKSNLMPVHTGHKAYSLLDNPEERTPIETQNYGTSL
                     LEYAYEPYMNLRSAIRSLKATRFNASKIDRIIGLAMNSLDPVKAADYSRTITQTLKRA
                     ADLMERRARGANNMPTVTNTLLPIMGDGKGQMTIDTQTIQADINGIEDILTYMRQLAA
                     ALGLDYTLLGWADQMSGGLGEGGFLRTAIQAAMRASWIQQGVEEFIQRAIDIHLAFKY
                     GKVYPEGDRPYKIEFHSVNTALQQEHNDNRDSQANYATIVTQILDAVSNNSVLANSDA
                     FKRYLFSDVLEIDEKISEALVNELKAKSEDDDHLMDSIIKTPPQELAQILESVFKEGN
                     DND"
     gene            30834..31853
                     /locus_tag="POB34_000871"
     CDS             30834..31853
                     /locus_tag="POB34_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head processing protein"
                     /protein_id="HDI5917863.1"
                     /translation="MTDVLKTVTDRFCLYSNARKGRQNGRQYVLSAVKTMLESKETQE
                     GLRLGELFGYYGHGRRQLTGKLEVPETSVIMVEGRPVVIDNVPACRTVAISVDDNGIV
                     THTQEILNTEPGKIVAAMIESRAGGWSWATGGRESGKIAVTTSFHGVDYVTTPNYISL
                     DHPASAGMFESADSKSLLAESLAAHGYSDESVQAVISHYGKMAELEMMVEATERTAEL
                     ETALLESQGRHLEAMAKIADAEARIALLEKTAGIRDDVLAAMQDELDNLPIFVSAAQK
                     DAFRLKEPGDAKIVATLFESLIKVGARNLPVTKKIKEVPQAANVQAPRETSIITFNNS
                     INPFK"
     gene            complement(32145..32702)
                     /locus_tag="POB34_000872"
     CDS             complement(32145..32702)
                     /locus_tag="POB34_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDI5917864.1"
                     /translation="MKGKTAAGGGAICAIAVMITIVMGNGNVRTNQAGLELIGNAEGC
                     RRDPYMCPAGVWTDGIGNTHGVTPGVRKNDQQIAADWEKNILIAERCINQHFRGKDMP
                     DNAFSAMTSAAFNMGCNSLRTYYSKARGMRVETSIHKWAQKGEWVNMCNHLPDFVNSN
                     GVPLRGLKIRREKERQLCLTGLVNE"
     gene            32872..33360
                     /locus_tag="POB34_000873"
     CDS             32872..33360
                     /locus_tag="POB34_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI5917865.1"
                     /translation="MAQRGVNKVILIGTLGQDPEIRYIPNGGAVGRLSIATNESWRDK
                     QAGQQKEQTEWHKVVLFGKLAEIASEYLRKGSQVYIEGKLKTRKWTDDAGVERYTTEI
                     IVSQGGTMQMIGARRDDSQSSNGWGQSNQPQNHQQYSGGGKPQSNANNEPPMDFEDDI
                     PF"
     gene            complement(33594..34706)
                     /gene="ompC"
                     /locus_tag="POB34_000874"
     CDS             complement(33594..34706)
                     /gene="ompC"
                     /locus_tag="POB34_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDI5917866.1"
                     /translation="MKKITVALSAVAASVLMAMSAQAAEIYNKDGNKLDLYGKVNAEH
                     YFSSSASDDGDKTYARMGFKGETQINDQLTGFGQWEYEFKGNRTETEGSDKDKTRLAF
                     AGLRFGDYGSLDYGRNYGVAYDVGAWTDVLPEFGGDTWTQTDVFMTQRATGVATYRNN
                     DFFGLVDGLNFALQYQGKNDSAAKVNNWKGRDVVESNGDGFGLSATYDYEGFGIGATY
                     AKSDRTDGQVSYANASSLNASGKTAEVWATGLKYDANNIYLAATYSETQNMTVFGDDF
                     IANKAKNFEAVAQYQFDFGLRPSIAYLHSRGENIGAFGNQDLVEYIDVGATYYFNKNM
                     SAFVDYKINLIDESEFTKASEVATDNIVAVGMTYQF"
     gene            complement(35446..35751)
                     /locus_tag="POB34_000875"
     CDS             complement(35446..35751)
                     /locus_tag="POB34_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917867.1"
                     /translation="MQIKIAAPLGGDAIIEFDDNEEVSGRLSIISGDITEDMIAEAIA
                     GANPNSYMGFVNTLDAPASDVLRTLHLYAGWFVDWPAVEGGDEDDDDFGDHVDQIVY"
     gene            complement(35741..38728)
                     /locus_tag="POB34_000876"
     CDS             complement(35741..38728)
                     /locus_tag="POB34_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917868.1"
                     /translation="MSLSDQVVMATSIETLIELLKNLPDYGRVSYVVTAKGDEVKTAF
                     DIVDASALLVSNTLDGKINPDYPQELQPRDRTRASSLLQVNQISKDLRPAQLTDSGLS
                     SHGAPIIGEDNAVESGNGRTMGIIKAYQDGNADRYREYLIDHATEFGIRPEKVESMTA
                     PVLVRRRLTKVDRVQFAKDSNISDLQEMAASEKAFVDADSITPAMMALFNPSESGDLL
                     SRSNDAFIRGFMTQVGATQAAGLVTEDGRPTRQLVDRIQNAIFAKAYKDARLVRMVAE
                     EPDPDMRNVLTALNAAANDFVQMQALSGEAHKQAVTTIVDGIETADSLDKKALAALKD
                     AVDLVRQSKESGQHITDVIAQGDMFSETAPEVKALALFIVANNRSAKRMATAFKLMAQ
                     RINDELQHQGQALGDMFGGGDVSLQDILRQVSQELENEGMQGISGGLFESVSGGSYNG
                     VAPYTSLLLHRASGIKDIIHLIRLLSRTDPQDEQLVQVLAHFVRMPVADVKKWCRLFG
                     ISNSLLRGLLNHASSLGRDGFDEIAQAIKNGDMPPAIDWFSIRPTRVKAFLSAAHSAS
                     PLAEMVQRLSLIFTDHTALGDLTLDEMKEASIQWADQQNEVNSDFLPAFRKAVSKADD
                     ARGILKAFKALQSRVNKHVGDIDGVTAEGRDILKEHGITPEFIDEIRTDMQREVVSSL
                     QIVARALADANPKSAAIVNRVIGDIEASEGMGALKLFLSRAFNPNGNILPGIIGEAKR
                     YVSEEELEQLDQLLKRFSYNPQTRWQMNQRSMGSVHEKVLSAMNSAIANSSVSEEKAL
                     EWADSFITEEVEEARAGQNGGIDLRKELADIYRLTGGKISTLSKVVHHQGRAYANLNG
                     VVAVNLNDENASALWHELGHHLEYSNPGLLEKARSFLKANVEGDKPSFVNIGGRGKPE
                     WCFRSRLSNIYMAKVYPPASVSNSGKIRQKAPTISKTSATEVFSMALQLYHDKEAAAA
                     SLMNGDGLLELLLGVAKELNNAD"
     gene            complement(38741..39106)
                     /locus_tag="POB34_000877"
     CDS             complement(38741..39106)
                     /locus_tag="POB34_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ddrA"
                     /protein_id="HDI5917869.1"
                     /translation="MTLSAIELMDLSDKLDALMSKAATASGMELLDISDEIDQIMQQM
                     GYGASGGSSGEEKQPSVHDGVPKLVADFLADKFVDQSTDAFIGTLQDLSQYVGTYIDL
                     DQVKQHTAAWIAANIEEAA"
     gene            complement(39103..40986)
                     /locus_tag="POB34_000878"
     CDS             complement(39103..40986)
                     /locus_tag="POB34_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917870.1"
                     /translation="MEQFNINKGVTIKPGLDVLPPPVTDDEYRALMAGEDRYLMTESN
                     TLEEIEATFFYDTPIHWCATDLLEAISSTRLQLHRTMQAFVRALNQKLNGTGISAGSD
                     KTGDVAQSGARAIGGAEIGRARNVNGLPVLPAIIPLSDGQTISILFHSPTAENRITNS
                     DTLVAFQFLLNKKDVTHTVAPMSGRDMTLAQVTMKLANLAEKNSSKFQRAQKKKKALV
                     DEITQLQADSDQKEDAMSDLADQVAAVEGQKADLEQKINAVASEADSLYEENERLQTE
                     IDQLNRTGGHSRALTDRLASIKNRMHMDGEATLSNGASMKQFIGDGEGYIQLTDPDGS
                     VYMIKAKSIQGVDMADAIGKLFKAYKAGNVSEYLVQPEEHKPENVEPEPAEDTGSSSP
                     EPEVSVGAYRYALQMRPAAPGAIPEGNKAILPRPDEGDPYYEYARYGIATYDTPLSDQ
                     QMSEYDLKLLPREDSFDFLAKTLTNGPFGKYAQKALELATSSPDEFRVMLKTQFQKTF
                     PNIAFPGGAGTEKMVQSMINALQAEVGEITQPEPAPAQPDETVSEADAEANKAIEYLN
                     NVMDIQSTDMAEIRNARGNVREAIAALQAAGRFEENEELVNGAARHLADLLVAIQKAG
                     VAA"
     gene            complement(40988..41590)
                     /locus_tag="POB34_000879"
     CDS             complement(40988..41590)
                     /locus_tag="POB34_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="odaE"
                     /protein_id="HDI5917871.1"
                     /translation="MTKNKYATVDFDQVNEKGLKSLIAAINKTGVTVIEVDSSNRATT
                     KDGVKVKTAKLVLNDGQILAIQVNDTGDISSVKLNGKAIPNAQSPDIKTLGTVMGQAA
                     RKNSAKFQKSLIAKAKRVANPVDKKPAVKSNFQRLQEAKQRNAQVVAAYKSAQNSVSF
                     TQQQITDLRAKLDKETGRLNNEKARNGELKRRLKQLKAGN"
     gene            complement(41577..42020)
                     /locus_tag="POB34_000880"
     CDS             complement(41577..42020)
                     /locus_tag="POB34_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HDI5917872.1"
                     /translation="MIGWGVCVLALALADRYLLKRKDITHLELGDVEIKPGFIRVPFK
                     YRSKFPFLRGATVRYWIRDVQKPTTVIEGEQRCLTSAEQGENSEWLYIPTEYMGKGER
                     LWHFNVMVTHGDSFINPLYRIFPVTQQIRRSYVINLAQDVSDDEK"
     gene            complement(42017..42346)
                     /locus_tag="POB34_000881"
     CDS             complement(42017..42346)
                     /locus_tag="POB34_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HDI5917873.1"
                     /translation="MLDTQELAPVAIALLLSVIGGIGTFLMDVRDGRQSGNLLGLVTE
                     IFVAVTAGAVAYLLGQHEGWELSITYLMVTIASNNGHEVISGMKRVNIDSILNVLTSL
                     VKKGGGK"
     gene            complement(42407..42691)
                     /locus_tag="POB34_000882"
     CDS             complement(42407..42691)
                     /locus_tag="POB34_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917874.1"
                     /translation="MQILIFLMSIISGTYLFLTGVRRGAYKPCFLHWLEAWLLAIKGG
                     FVGFCLAEVQWGNSFLDVVAFLICSWNSHFVLRITKIWVFHLIYECIGKL"
     gene            42813..43373
                     /locus_tag="POB34_000883"
     CDS             42813..43373
                     /locus_tag="POB34_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI5917875.1"
                     /translation="MLIGYVRVSTNEQNTALQRNALESAGCELIFEDKASGKQTERPG
                     LKKVLRMLSRGDTLVVWKLDRLGRSMRHLVVLVEELRDRGINFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLDAARAEGRIGGRRPKYQEETWQQMRRLLENGIP
                     RKQVAIIYDVAVSTLYKKFPASSFQS"
     gene            43421..44722
                     /locus_tag="POB34_000884"
     CDS             43421..44722
                     /locus_tag="POB34_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI5917876.1"
                     /translation="MSTANTWSARQIFNGGITGALTGNADTATKLKTARTIGGVAFDG
                     TANINLPGVNVAGNQNTSGNAATATKLQTACTINGVSFDGSKNIELTAEDLNLQEFIN
                     KANNAVQRSGDILSGGLTFENDSILAWIRNTDWAKIGFKNDADSDTDSYMWFETGDNG
                     NEYFKWRSKQSTTTKDLMNLKWDALSVLVKALFSSEVKISTVNALRIFNSSFGAIFRR
                     SEECLHIIPTRENEGENGDIGPLRPFTLNLRTGRITMGHGLDVTGDITTNAWVYANRF
                     AINSGSTSWIDMRNQNVIFGRNAVSTSSAQALLRQDHAERKFFVGGLGNYQFGFYMIN
                     NSRTANGTDGQAYMDNNGNWLCGSQVIPGNYGNFDSRYVRDVRLGTRVVQLMARGGRY
                     EKAGHAITGLRIIGEVDGDDEAIFRPIQKYINGTWYNVAQV"
     gene            44726..45253
                     /locus_tag="POB34_000885"
     CDS             44726..45253
                     /locus_tag="POB34_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDI5917877.1"
                     /translation="MQHLKNIKSGNPKTKEQYQLTKNFDVIWLWSEDDKNWYEEVKNF
                     QPDTIKIVYDANNIIVAITKDASTLNPEGFSVVEVPDITANRRADDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(45282..45815)
                     /locus_tag="POB34_000886"
     CDS             complement(45282..45815)
                     /locus_tag="POB34_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDI5917878.1"
                     /translation="MMHLKNITAGNPKTKEQYQLTKQFNIKWLYTEDGKNWYEEQKNF
                     QPDTLKMVYDHNDVIICIEKDVSAINPEGASVVEVPDITANRRADISGKWMFKDGVVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(45818..47119)
                     /locus_tag="POB34_000887"
     CDS             complement(45818..47119)
                     /locus_tag="POB34_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI5917879.1"
                     /translation="MYEAYNNGYPTTYGNVLHLKGAASTGEGELLIGWSGTNGAHAPA
                     FIRSKRDSTAAAWSEWAQIYTSKDSVPGVNTKGNQDTSGNAATATKLQTACTINGVSF
                     DGSKNIELTAADLNLEQTVELAAGALQKNQNGADIPGKDTFTKNIGACRAYSAWLNIG
                     GDSQVWTTAQFISWLESQGAFNHPYWMCKGSWAYANNKVITDTGCGNICLAGAVVEVI
                     GTRGAMTIRVTTPSTSSGGGITNAQFTYINHGDAYAPGWRRDYNTKNQQPAFALGQTG
                     SRVANDKAVGWNWNSGVYDADISGASTLILHFNMNAGSCPAVQFRVNYKNGGIFYRSA
                     RDGYGFEANWSEFYTTTRKPSAGDVGAYTQAECNSRFITGIRLGGLSSVQTWNGPGWS
                     DRSGYVVTGSVNGNRDELIDTTQARPIQYCINGTWYNAGSI"
BASE COUNT        12320 a        11357 c        11108 g        12556 t
ORIGIN      
        1 gcacaaaacc aatcaatcga aaatgtatct atccctgatg tcctgaatgc cggtatcccg
       61 gccattatcc agaacatccg ggccgcgcaa cgccgcgtta gttgtgatga cctcacagcg
      121 cgtttttttg ataatgcggt tcagtcagcg gagatgcttc acgcacagct tattgatgtt
      181 tataacgcag aagctgatag ccataactcc ctggtagatg cagctgaaaa tatgcagttg
      241 gatctcggtc tgaagggtaa agaaattgaa gagcttcagc tgcaaattga acatttgaaa
      301 cgccagcaac aggacgcgat cgacgatgcg acgcatgacg ccaaccagcg tgctgataat
      361 gccgaacgta taagcattga gctggaaaca aaactcaatg aaatgaccgc gatggttgaa
      421 ctgcggaact cacagatttc aacgctaaaa tctcaatata aagagatcat gaaacttgat
      481 ccttttaacc ttgagaaacg ctataacaaa gctaaaagcg agcgacagga actgcgtaag
      541 caggtcgccg accttaacca acagctcaaa aaaactatta aagatgcaag cgaggcgcgc
      601 gtggcatttg ctaataaaaa agcagaggtt accgcgctgg ttaatgagaa tgccaaattt
      661 gcgacgctca agaaggaaat gtatggcatt actgagcgcc gtttccctgc aagcaaactt
      721 catccgacgt tagggcaaat ctccttcttc ccgcgcctcc tggcttatgg gatctcatcg
      781 cctaaagagt tcaataacga gcgtccttat atcgtttcta agctggactt tgcttatcag
      841 ttctgctgcg acatgggcta tgccattgat atccgaatca acgaatggtt gatgccaaac
      901 ttccagccgt tggcaatttt ccgcgagttc cagccggaag gttgggtaga gttcttccat
      961 gaattgatct gtaaagagat ggaaagccgc cgcccggaac tggtccgccg tgtcgagtgg
     1021 gcgcaagagg ttatgttggc agatgcagag ctgccgttcg aaccggaatt cattgatgat
     1081 ctggcaacta aagggctgca taccctgttt gatgtggtta cccgccgtca tgagcagttg
     1141 gttgtcgaat tgggtttaga ggaaactgcg gcaagaagac ttctcgatgt ttgctatgca
     1201 cgtagcgatg catgggaaaa agagaacggc ggcactattt acgttcgctg atagttacag
     1261 tgtcactttt aatgctggtg gagtgctccc accagcattt ttttcgtcca atgaggaggg
     1321 catttgagta ttttcaataa acacgcacac caggaacgtc cgtatatcgt catagtcgat
     1381 attgatggaa caatatcaga ggcaactgaa gacagactgc atttgcttcc gccaccaggt
     1441 aaaggtgcat taacaaagga ctggaacgag tttaatctcg cctgtgacac cgatactccc
     1501 atcactccag ttattgatat ggtgcgccag ttatttaacg tttacacggt ctggtttgta
     1561 accgggcgct gtgagatcgc aagggataaa acacgagcct ggctgcggaa gtacgtaaca
     1621 aacggggctg agcctttgct atctatgcgt cctgccaccg atgacagaaa tgacggccca
     1681 gcaaagattg atctccttaa gaaaattggt ctaagtaaaa tcgcgttcgc gctggaagat
     1741 aagattgaag tggcgcgtgt tttcaggagg cacggcgtgc ttacgttaat ggtcagggag
     1801 tatgaaaatg cgcttcttca tcagcaataa ttgctcaaat aaatattgat ttttaaaaca
     1861 gagaaagtga aaataaaaac atgccgcaag gcgcggcatg tatccaatca atcacaggag
     1921 ctgaaaatat gagcacggca ttcaaaatca ttatggccgc gatctatttc tggctgttct
     1981 ttatcacttt tggcagcatt gtcgcgcata ggtaagggga gtatattagc catttggaac
     2041 cccacggtct cttgcgggtt taattaagaa cccgcaagaa aaacacgaat tgggctatat
     2101 ttttccgcct acgcctttaa acttctcaat aaacgagacg attttctgga aaactgcctg
     2161 ttttttcgtt ttatattgcg gattcaacgg actaagtttt ggtaatgttt cgtttaattc
     2221 tgtgccattt tcggtggcat attcgcgttt taaagacgtg cgaatatagc gttttgctgc
     2281 atcttcattg agattttctt cttttatcaa tgcttctgct tcacgttgct gttcgcgttg
     2341 agcaaacgta aagaatgcgt caatgatgct ggctttgtcc ggtaaatcat ccaggttcgt
     2401 ttgctgaata aaatcgacca ccaggccctc tttagcacgg ttccctaggc ttgaacgaat
     2461 taagcgtttg acctcttcga tcatttcgcc cttgccttta ttttgtctgt tgtgttcgaa
     2521 aatcagtcca aggatataat ccaggtttat ttcctgagac ttcagcaaat cgacctcaaa
     2581 aactacgtca tcccagtcag tggttgattt ctcttttttc tcagcttctt tctcacggcg
     2641 ctgccagtcg cgaatatcgt tataggcaga acgataatcc tgaatcttgc gatcagcagg
     2701 gagacgaatt gtttgcaatt cagcgaactt ttcatcatcc acataatgtt ctgctttgaa
     2761 tttttctacc gcaacaggat cgctaagatc gatttgttgc agggctttta gcgtggcaaa
     2821 ttcatcatag ttttgcagga tgttctcggc acgcaggtat tcgccaaaca gtttaacgaa
     2881 gtctttcttc tctttttcac tttcaatact ggtagggtca gggaaccgtt gttccagttc
     2941 tgaaactact gtcatgaagc cgcgtttagc ttcaccagta gtagcatcag taaagccttc
     3001 catatactct gcataactct tttctaaaac cacatttttg gtgtttttgt caccaaacag
     3061 cgttatggca tcaatagttg agcgttccag atcccggaaa gtgacgatat taccgaaggt
     3121 tttagttgca tcataaatac ggttggtgcg ggaaaatgcc tgcatcaagc cgtgaaaacg
     3181 caggttttta tcgacgaata gcgtgttcaa tgttggagca tcgaagccag ttaaaaacat
     3241 ccccacgaca attaacagat cgatatcctg atttttaacc cgttgggcta aatcgcgata
     3301 gtagttctga aaaccgttac cgtcggtgct gaagttagtt ttaaaatagc tgttatactc
     3361 acgaattgca gcgtcaagaa actctttagc actgctgtcc attgcgctgg tatcaaaagt
     3421 ttcatcgcaa atttcaccaa tggcattttg ttcttcattg gcggcaaagg agaagattgt
     3481 cgcaatacgc agcggtttat aggtagccga tttattagcg gcttcttctt gtaaccgttt
     3541 aaacgtcgca taataggttt tcgcggcatc cacgctgctc actgccaaca tagcattaaa
     3601 accttttgag ccagggaagg tacggtgagt tttctggcgg aaattattca gaatatattg
     3661 cgtgatttcc ttaatacgca tgggatgaag aaacgcctgc tgattttcag ccgcacttag
     3721 ttttttctcg tcagtttctg tctctaaaga cttaaactgt ggccgcacat cgttgtagtc
     3781 caccttgaat ttgagcactt tttcgtcacg aatcgcatcg gtaattacat acgaatgcaa
     3841 ttcacgacca aatacgctgg cggttgtttc tgagcctaag gcgttttccg ggaaaatagg
     3901 ggtgccggta aaaccaaact gataatagcg tttgaatttc ttcttcaggt ttttctgcgc
     3961 ttctccaaac tggctgcggt ggcattcatc aaatatgaac accacttgct gattatatac
     4021 aggcaggtcg ctttctgctt tcatcaggtt attgagtttc tgaatagtag tgacgataat
     4081 tttgttatcg tccttatcca gatttcgttt aaggcctgcg gtattttccg agccgttgac
     4141 gctgtctggc gaaaaacgct gatattcctt catggtctgg taatcgaggt cttttctgtc
     4201 gaccacaaag aagactttat caataaagtc cagctctgtt gccagacgcg cggctttaaa
     4261 gctggtaagg gttttaccag aaccggtagt gtgccagata tacccaccgc tttccggttt
     4321 tgaccagttc ttcgctgtaa aggagctctt aattttccac agaatgcgct cggtggcggc
     4381 aatctggtac ggtcgcatca ccagtagcgt ctgactactg tcaaacacgc tgtagttcac
     4441 cagaacattc agcagagtat gtttctggaa aaaggtagcg gtaaagtctt tgaggtcttt
     4501 aatcagcgtg ttgtctgatt tcgcccagtt catggtgaaa tcaaaactgt ttttatcgcg
     4561 ctttgtggtg ttggcaaagt aacgggtatc ggtgccgtta gaaatgacaa acagttgcag
     4621 atacttaaac agggaatttt cgctgttaaa actctcttta ctgtaacgat gtatctggtt
     4681 gaaagcctca cgaatcgcca cgccgcgctt tttcagttcg atttgtacca gtggtaagcc
     4741 attaaccagg atcgtgacgt cataacggtt agcatgagaa cccgtctgtt caaactgctg
     4801 gataatctgc accttattgc gcatgagatt ctttttatct atcaaataga tgttctcaag
     4861 tcgcccgtcg tcaaaaataa agtcgcagat atagtcgata tggattttac gggttttatc
     4921 cagaatacca tcgctggggt tgtccagata ctgctccgtg aagcgccgcc actcgctgtc
     4981 attaaacatc acaccattga ggctctgaag ctgttcccga acattagaca gcattgccga
     5041 ctgtgatttt acggaaataa attcataacc ctgattccgc aggtcctgaa tcagttcacg
     5101 ttccaggtcc gattcgctct ggtagctgtc gcttgtttgc tcagctttga tgtacttatc
     5161 aaggacgata aagttattgg attcagcaat ggtgtgtgtt tgatgagtca tagcgcatcc
     5221 tttgtgccgc ctggcaagag ccggaagggc gttaagagtg acttccggcg cgtaaaaaat
     5281 agtctatata ctgaccgggt gttaaggtgg cccgatcggt agcaatggtc aattaattac
     5341 tgacagtttc aggtttcggg aaactgaaca gtaaatcacg gtagtattcg tattgtttct
     5401 ggcgcaactc gatttcacgc gggagacctt cagtgatgga gttggtcagg gtgtcgaatt
     5461 tatcgagtat ttcgacaatg cgagcttgtt cttctgaagg tggtacaggt attgggaatt
     5521 ttaaaaacat tggtcgacga agtgaggtaa cagatgaact tactgatgct ttttgaatat
     5581 aggtaaaaaa atatgctgtt atatagtgat atacaaatcg cggattcata aatgcatttg
     5641 ggacaatacg atatgctctc tgatgcaaag catatttacc tttagcgtaa tgaaatacct
     5701 tccccacccc ggcaccatct ccagctgtaa tgattgctgt ttcatcaaaa tcaaatacat
     5761 ttaactttaa tggctcacgt ccacgtgcat aaaaaatata tttgccatgt tctatagcat
     5821 cttgcgtatc atggttacct gttcctataa cagccacttc ccccaaagtc ttccactcaa
     5881 cctcaccctc tttaaaactc aacaactggt cacgatagta gttgtactgt tttttacgca
     5941 tgttaagctc agcggtaagc tcagcggtaa gtgcagtaaa tttatccaga atccgcacga
     6001 tttcagactg gatggtcaga gacttttctg gattgtttga actgggaatt ggaattttaa
     6061 ttttagataa atcgccactg gaaacccgtc ggactttagc tccagtaata tatttcattt
     6121 tttgtgtttg aaatatttcg gactggaaaa aataggcgag atatttaaca ttttgctcgt
     6181 ggcgaaataa catcatatct ccggaaatag caacgttatc gccaagccag gctaaaggtt
     6241 ttactacatc ttcatcgttt tctgatgttg ttgctagcaa taagtcattt ttttgagcct
     6301 ttctcagctt attggctagt tctggaagca cgtaagtaaa tgttttatca gctgataacc
     6361 cataccttgt ataaatctga ccgtaatgta ttgctggaaa accactttca acgaaatctt
     6421 ttttctgtag tccattgcca cgtatgaaat cgccaagctc tcccagtgac aaccattcaa
     6481 catcaactcc atccagcaat ttgttcagaa agttcaaccc actcatgatt gcgcctcgta
     6541 accttcaatc tttgccacaa ttgagtcgat atccttacgt aactgttcga ttttgccgac
     6601 ggtgcttttc agctctgcat tcaactcagc gatatcgata atttcgcgag tatctttcgc
     6661 ttccacatag ctgctcaccg acaggttata gtcattcgcg acaacagtct caaacgcaac
     6721 agatttcgcc agatgagcaa catcttcctt gctggcaaat acctgcataa tctgttcgat
     6781 atgggcatcg gtcaggatat tgttgttggt ctcttttttg aacagttcgc tggcatcaat
     6841 aaactgaacg ttggtatccg ttttatgttt ggacagaacc agaatgttta cggcaatggt
     6901 ggtgccaaag aacaggttcg gagccagtga aattacggtt tcgacatagt tattatcgac
     6961 cagatactga cggattttct gctctgcgcc gccacggtaa aaaatacccg ggaagcagac
     7021 aatcgcagca cgacctttgg ccgaaagata gttcagcgca tgtaatacaa acgcaaagtc
     7081 agctttggat ttgggggcca gaacgccagc cggggcaaaa cgctcatcgt taatcagcgt
     7141 cgggtcatcg ctgccaatcc atttcaccga atacggcggg ttagaaacga tggcatcaaa
     7201 cggtttttca tctctgaagt gcggttcagt cagcgtattg cccagcttga tatcaaactt
     7261 gtcgtagttg atgttgtgca aaaacatgtt catacgcgcc aggttatagg tcgtatggtt
     7321 gatttcctga ccgaaaaaac cttcttcgat gatatggtta tcaaactgtt ttttcgcctg
     7381 caacaacagt gagccggaac ccgctgccgg gtcgtagatt ttgttaacgc tggtctgccc
     7441 gtgcatagcc agttgtgcaa tcagcttgga gacgtgctgc ggtgtaaaga actcaccgcc
     7501 tgacttaccg gcatttgccg catagttaga aatcaggaac tcataggcat caccgaacag
     7561 gtcaatctga tgttcgttga agtcaccaag ttttaaccct tcaacccctt tcagaaccgc
     7621 agccaggcgg gcatttttat ctttaacggt gttacccagg cggttactgg tggtgtcgaa
     7681 atcagcaaac aaacctttga tgtcagcttc tgaaggataa ccgtaagcag aactttcgat
     7741 agcaacgaag atactgttta aatctgcatt caatctgtca ttggtatttg ctttcgcagc
     7801 tacgttgcag aaaagctgac tggggtagat gaagtagcct ttagttttga tggcatcgtc
     7861 tttaatgtca tcagtaatta cgctgtcatc cagtttcgca taacagatac tgtcatcacc
     7921 ggcttcaata taactggaaa aattttcgct gataaaacgg tagaaaagtg cgcccagaac
     7981 gtattgctta aaatcccatc catcgaccga acccctgaca tcgttagcaa tttgccagat
     8041 ttgacgatga agctctgcac gttgttgaat acttgtcatt ttcatccact tatttcaggc
     8101 ttatgtaatt ggcggtgatt ctacagcaac ttggatgctt tagcagttcg gacattaggc
     8161 tacgaatgac ctgcctagag gtttgttaag ccgcaaagtg ctggtgcttt atgcctgtga
     8221 agtttataat tgtgtacaca taacgagtac acgaggtgtt tatgcaatcc attaacttcc
     8281 gtaccgcgcg cggcaacctt tctgaagtgc tcaacaatgt tgaagccggg gaagaagttg
     8341 aaatcacccg cagaggccgt gagccagcag taattgtcag caaggctact ttcgaagcct
     8401 acaaaaaagc ggcgctggat gctgaatttg catccctgtt tgacaccctg gactccacca
     8461 acaaggaact ggttaaccga taatgaggca tatatcaccg gaagaactta ttgcgcttca
     8521 tgatgcgaat ataaaccgct acggcggcct gccgggaatg tcagatccgg gtagggcaga
     8581 ggccattatc gggagagttc aggccagagt tgcctacgaa gagatcaccg accttttcga
     8641 agtctccgcc acctacctgg tggctacagc gagagggcat atattcaatg atgccaataa
     8701 gcgtaccgcg ctaaacagtg cgctgttatt tctacgccgt aacggggtgc aggtatttga
     8761 ttcacctgaa ctggcagacc ttactgtagg cgctgcgact ggcgagatat ctgtatcttc
     8821 tgtcgccgac acgttacgta gattgtatgg ttctgcggag tagattaatg gcacgcaaat
     8881 acaacaaatt gtcccgtgaa gcgttaaaga tgcttcttga tggcgtgagt cgccgcgagg
     8941 taaagcaata cctggttggt aagcaagttg gtgtcaggac cgctattgct gtgttatgcc
     9001 gtcaggaaat ggttgtgctt aaacagagaa tgccgggcag cagataaagc ccaatcagtg
     9061 atgaaaggtg tgatgtgaaa gccattatta ctccctttgt acaaaaagag cttggcgtcg
     9121 ccacattcaa agtggatcag gaagtcagaa agctggtgga ggctggccgt aaatttatta
     9181 tggagccggt gccgcgtgag ttaatcgagc acatggacga cggcctcgtt gtttccgagc
     9241 aaaccatggc aacaaatgag gcgttgcagc cgttttttaa cagcgatgag ctgtttcgtc
     9301 gtattggtgg aattgacgcg ctggtggcgt ggttgcgcag gaaagagggg cgatgccagg
     9361 ccgcagatcg tagctggtgt gacaaccata ttgtccacgc tgaacgagat aatagcgcgg
     9421 tgttgttgtg ctggcatcac gataaccatt accggatgcg tggttttaat gagctgaaag
     9481 aaacgctgca taataatcgc gttaactgga tactggatgt cgcccgtcag gaaatggggc
     9541 tttcagatgg ccatgattta agtattcagg aactgtgctg gtgggctttc atgcgcgaca
     9601 tgatgcacct gatgccggaa gaagtctgcc gtatatcaat aaataagatt aaggctgcaa
     9661 cgcaggatag cggacctatg aaagaggcgg atattcgccc gtatgacgat cgcgctacag
     9721 catatgttca gatgatggaa gaacgcgccg cgccgatgcg tgcaaaagta tgccctgtgg
     9781 atgttgactc cgaccctggc atggcgcatt tcaaaatacc aaaactgcaa tcgctaaaat
     9841 tacctgagta catggacttt gttgcttccc gtccatgctg tgggtgtgga gcggcgggag
     9901 ctggcgctca cattacgcct tatatcgttc gtcatagtcg attatgcgcg catgacattt
     9961 acgcaattcc tctgtgccag tcatgccagc gtgatattga gcgtgaccgc gataattggg
    10021 agaagacgca cggtaggctg gcgatgcatc aacgattgtt ctttgattac gcgcttggag
    10081 tcggcgctat cacaagtcac tcgtcgagcg ttagataaaa ttgctctaat gtattgctat
    10141 ttctttaatc gagggtatta tattccacgt tgattagttg acatgggcta atcagtaggt
    10201 gacaggatgt tacttaactg gcagggacgc cacttcatgg aaataaatca ctcacgaata
    10261 acatcgtacg agattgcgga ttacatgatc cgcactaaat ctcttctatc agcgaaagaa
    10321 ctcgcagcaa ttcttgaaaa ggaatacccg catctggatg tcgataagcg cgatgtttat
    10381 ctgcgcttaa aggctatcgc tgtgtctaag tattcgtctg ttttgattga tgacagtaca
    10441 cgcccacgta gatttcagat ccactctctg aatcctgaat tctttcgccg tagccgcgct
    10501 ccgcgccggt ttgatgaaaa actccagaac gaactctata tgacgcagga cgaaaaggaa
    10561 cgccgggagc accaaccttg ggtaatggcg cgtcaacttt tcaataaggt ggcccgtcag
    10621 caccgtcatt acggtaatgc cacatccgca cgtatctgat tgattgcttg cccgttccgg
    10681 gccttttgac atgtgacttt cgttaccctc gcgtcaaaaa gagtttttac gaaaggaagc
    10741 ataagtgacc tgggacgatc acaagaagaa ttttgctcgc ctggcgcgag atggtggtta
    10801 caccatcgca cagtatgccg ccgagtttaa tcttaaccct aataccgcac gtcgttatct
    10861 ccgtgccttc aaagaagaca ccaggactgt ggacagccgc aagccaaata agccagtcag
    10921 gaagccacta aaaagcatga tcattgatca ctctaatgat caacatgcag gtgatcatat
    10981 tgcggctgaa atggcagaaa aacaaagagt taatgctgtt gtcagtgccg cagtcgagaa
    11041 cgcgaagcgc caaaataagc gcataaatga tcgttcagat gatcatgacg tgatcacccg
    11101 cgcccaccgg accttacgtg atcgcctgga acgcgacacc ctggatgatg atggtgaacg
    11161 ctttgaattc gaagctggcg attacctgat agataacgtt gaagcgcgga aggccgcgcg
    11221 cgctatgttg cgtcgttccg gagctgatgt tctggaaacc actcttctgg aaaagtctct
    11281 ttctcatctc cttatgctgg agaacgccag ggatacgtgt attcgcctgg tgcaggaaat
    11341 gcgcgatcag caaaaagacg atgatgaagg tactccgcct gaataccgta tcgcgagcat
    11401 gctaaacagc tgttccgcgc agataagcag cctgatcaac accatttaca gcatccggaa
    11461 taactatcga aaagaaagcc gggaggcgga aaagcacgct ttgtctatgg ggcaagctgg
    11521 cattgttaag ctggcatacg aacgaaagcg tgaaaataac tggtcagtgc tggaagcagc
    11581 tgaattcatc gaggcgcatg gcggaaaagt gccgcccctg atgctggagc aaatcaaagc
    11641 cgatctgcgt gctcctaaga ccaataccga tgatgaggaa aggcaaacag ccgtcggtgg
    11701 cccttctctt gaagatctgg acaaagttgc gcgagaacgg gccgccaacc gccgcgccga
    11761 tgccgcattg tggattgagc agcgtaggga agaaatcgcc gatatcgttg atacaggcgg
    11821 ttatggagat gttgatactg aaggtgtatc aaacgaccca tggctggaac aagacctgga
    11881 cgaagacgag gaggaagacg aagaagttac ccgcaagcta tacggggatg atgattaatg
    11941 gccagaagtt gcgtaacgga tccacgttgg cgcgagctgg tggcgctata tcgttatgac
    12001 tggattgctg ccgctgatgt gttgtttggg aagacaccaa cctggcagca ggatgagatc
    12061 attgagtcca cgcagcagga cggcagttgg acaagtgtga catccggcca tggtactggt
    12121 aaatcggata tgacgagtat cattgcaata ctcttcatca tgtttttccc cggcgctcgc
    12181 gtcattctgg tcgctaacaa aagacagcaa gtccttgatg gtattttcaa atacataaag
    12241 agcaattggg ctactgctgt tagcagattc ccgtggttgt cgaagtattt cattcttaca
    12301 gaaacgtctt tttttgaggt gactggcaag ggtgtttgga caatattgat aaagtcctgt
    12361 cgccccggaa atgaggaggc gttggctggt gaacacgccg atcatctctt gtatatcatc
    12421 gacgaagcgt cgggtgtgag tgataaagca ttcagtgtga taacaggtgc gctgaccggt
    12481 aaggataacc gtattctgct tctttcccag cctacgcgac cttcaggcta tttctacgat
    12541 tcacaccaca gactagctat tcgcccggga aatcctgatg gattgtttac tgcgataata
    12601 ctgaatagtg aagaatctcc gcttgtagat gcaaaattta tacgagcaaa acttgcggag
    12661 tatggcggtc gtgataaccc catgtacatg atcaaagtac gtggtgaatt tcccaaatct
    12721 caagatggct ttcttcttgg tcgtgatgag gttgagcggg cgacgcggcg aaaggtcaag
    12781 attgccaaag gatggggctg ggttgcatgt gttgacgttg ctggtggcac aggacgagat
    12841 aagtccgtta tcaatatcat gatggtgtcc ggccagcgaa acaaacgccg tgtaatcaac
    12901 tatcgaatgc tggaatacac agacgttaca gaaacgcagc ttgccgccaa aattttcgca
    12961 gaatgtaatc ctgagcgatt cccaaatatc accatagcga tagacggcga tggcctgggt
    13021 aaagcaacag cggatctgat gtacgagtat tatggtatta ccgtacagcg tatacgctgg
    13081 ggtaaaaaga tgcatagccg tgaagataag agcctgtact ttgataaacg tgcttatgcc
    13141 aacgttcaag ccgcagaggc cgtaaaatct ggtcgtatga gactggataa gggtaatgaa
    13201 actattgagg aagcgtcgaa aatccctgta gggattaact ccgcaggtca atggaaagtg
    13261 atgagtaagg aggatatgaa gaaaaaactc aacctgcact caccagacca ttgggataca
    13321 tattgtttcg ctatgctggc ggattatgtt ccccaggacg aagtgcttag cgtcgaagac
    13381 gaagcgcagg ttgatgaagc tcttgcatgg cttaatgaat gaatatttgc tctaataaat
    13441 tgtgtttttt aactaccgat gttacattga acctgacctc ttgcgccttg aggcattttc
    13501 ggtttatgct tatcaggcac ctcagtaaaa cgggtgccgg gattggcctc ccgcttaagt
    13561 ctaaggcgat acagacgccg ctcgcgtctt ttttttgtat cggcgtacac gcacacctct
    13621 acaatggtgg gctgtatggg gctaccttcg ggtaggctgg ttaccttgga cgccagtaag
    13681 gccaactccg tacagtccac cgccagcaag attggtctct tctgcggtgg ttacatacca
    13741 acgtctaagg aggctgccaa tatggctact atccctaccc caactcatcc tgaatttatc
    13801 tggcgctttt actcctgcca aaaacgtcac tatcacttcg ttattgcacc gacagaagat
    13861 gaggcacgtt ctcagcttcc tgacgcccca tgtattttct ctgcccgttt ttccactgat
    13921 tcacgcaatt ctctcagtta ctggtgcctc cctgttaacg cttctgctca ggagggacta
    13981 tgagaacatc gttagttacc cgtgaagaga tgatcgaggc aattgaacag cacactgcct
    14041 gtatcagtac cagggatata ccaggcgtta ttgccaacta cttcatgatc accaaacaac
    14101 tttaccggag aaaggacaag aacgcggttc accgtatcct gttgtctgat atccgcgaat
    14161 acctgctcga acagggtcat ctgaattacg caaccgtcgc agccgaatca cgcaaggagg
    14221 cacacagaat gaaagcaact aacgttaaat cagaaaaaat tcatgcacct tcagttcagg
    14281 aatcggagct ggtggttgtt cagaatcagt ctgatgaaat tcccgttctg gaatggcagg
    14341 gagtgcgtgt agtgacaacc gagactcttg ctagagggta tgggacagaa acaatccgta
    14401 ttcgccaaaa tcatcatgag aacaaagtac gctttgttga agggaagcac tttttcaaag
    14461 ttgaaggaga atcattgcgc gagttgaagc acagagtagc tttaaactac tctgtaaaaa
    14521 ttgctcgcaa tgttcgctca ctcaccctct ggacagaacg cggcgcagcc cgccacgcta
    14581 aaatgctcga aaccgatcag gcatgggcat tctttgaaaa actggaagac agctacttcc
    14641 ggcaaaaaga acagcaaccg gttgcaatcc cccagacgct tccagaagct ctgcgcctgg
    14701 ctgccgaact ggctgaacaa aagcagcttc tggaacagaa agcccaccag ctaaatcagc
    14761 agctggtggc cgccgctcct aaagtcgatt ttgccgaccg ggtatcagta gctaaaggga
    14821 tcctgattgg gaattttgca aaggttgttg gacttaagca aaacgcgctg tttgcctggt
    14881 tacgggagaa cggcatcctg atagcgtccg gtggacgtaa aaatgtgccg ttccagcagt
    14941 acatcaatgc ggggtatttc acggtgaaag aagtggtgct ggatgatgaa gatggctacc
    15001 agatacggtt gacgcctcaa ttaacgggta aaggccagca gtggttgacg cgtaaactgc
    15061 tcgatgctgg cttgttaaaa ccggtggcgg ctgaataatg gaagaatgcc cggttgatgc
    15121 cgggcataat ttattgcgcg ctttcggggt tgtcgtttac tggctgcccc ttcttggttt
    15181 tacggctgcg cgtaactgat gcggctgact taaccttttt ctcttcgcga gtgatggcaa
    15241 tttgtttttt tacattttca atatctgcca ggcgatatat ttttgcttgc ggccagcggt
    15301 cgcagatgat cggttctatg gagtcataaa ggctaaattt tgctttttcg aattcaccgt
    15361 tgatgataat tccatcacgg agagtttcat cgcagataaa cacgccacac agtggcacat
    15421 ggtaactaac tgatttacca tcattgtagt tagggctact ggaaatgtag tggacgcgca
    15481 gcattgtttc gctaaagccg tgtacgcgca tacggaattt ttcatcctcc gggtactgct
    15541 tcattagctc ttttgttgct tccaggttct ctatgtattt cgcactgtgc tcattgaacc
    15601 ccgcgctttt ttggatgcga atgtccttat caatcagatg aataatgcgg ccagcggtca
    15661 tgttgacgct gttcacagcc tctgtctgat aagttgtaac cttacgcaca ccgcgaagga
    15721 tgttaggcac tggatataaa atagtctttg ggatattgag gtctgggtac tgttccagtt
    15781 cccgcgccat taaagtccat ttatcaattt cagcctgaat gctgtcagtt tctttgaacg
    15841 gtagaacgac aaccgggcgt acaggacgac cgtcgctggc tgtatcaacg tgttgggcgc
    15901 gtgcaacagc tttttttaga aagagatccc tgaagctgac gaactcctgg tacagttgtt
    15961 cgccgtagac ataatttatc attgatcctc ctccagaatt gacatggcca ataacgcccg
    16021 gctgagaaaa ccggtcatta ctgacctata ttatagaggg atcaaacaaa aataatagat
    16081 ttattagtgc atttattgtg agtctaactg gttagttgcc atgagatatt cgattgtgtc
    16141 agtgaggtca tccaggtcgt cttgggtgat gcggtactcc tgattggata tctttgagta
    16201 gtgttcagca atggcgcggg cagcgtcggt ttcggcaggg tctacagata aagcgttaga
    16261 gcaatgtcta acgtcgtcga tggttggttg aatgaaagcc ataattatgc ctcactgtat
    16321 tgacaacaca gagcctgaag ctctgaccta ctgtttcacc catgatccat gctggggtaa
    16381 tctaacaaca ttgcgctgtg tgtaagatga gcaatgcata gctgtaatgc cgttgtataa
    16441 ggtttccctg tttgctcatt tccttctgag ccgctctaca acgctgaaga cacattaaat
    16501 agtgaatcca aagtcgtatt acgtaacggc ggcaaaacta taatttatta gagcaattgt
    16561 caaacaacta tgaaaaacaa tccagttttt ggctggtgga gtgggatttt tctctcaaaa
    16621 tttattgctc taataattct tgatttttat gcgcagctgg acgtaaactc ctcttcggac
    16681 ctaataactt cgtatagcat acattatacg aagttatctt aagggttatt gaacatgatc
    16741 aatttacctg taaatccata cagttcaata ccttatcagg tcaaatagtg atcacttgat
    16801 catttgatca aggttgcgct acgtaaaatc tgcgaaatgt tggcagtgtt agtgctccag
    16861 atttcgcgta gcgcacttag caccaccaat caatcagagg tgaaaaatgg gatattcagc
    16921 tgctaaagtg tccactcatc ttgagcttga gaaaaaccgt ggttactggc gggcaaaagg
    16981 gtttgatcgt gatagttgtc aactgtcatt atcgcgcggt gaagagaaaa tagaacgcac
    17041 gcgcggtcgc tggcgtttct atgacgagaa ccataaacag gtaaaggcag agccgatcct
    17101 gtacacttta cttaaaacca ttatctgagt gttaaatgtc caatttactg accgtacacc
    17161 aaaatttgcc tgcattaccg gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg
    17221 acatgttcag ggatcgccag gcgttttctg agcatacctg gaaaatgctt ctgtccgttt
    17281 gccggtcgtg ggcggcatgg tgcaagttga ataaccggaa gtggtttccc gcagaacctg
    17341 aagatgttcg cgattatctt ctatatcttc aggcgcgcgg tctggcagtg aaaactatcc
    17401 agcaacattt gggccagcta aatatgcttc atcgtcggtc cggcctgcca cgaccaagtg
    17461 acagcaatgc tgtttcactc gtcatgcgac ggatccgaaa agaaaacgtt gatgccggtg
    17521 agcgtgcaaa acaggcgctg gcgttcgaac gcactgattt cgaccaggtt cgttcactca
    17581 tggaaaatag cgatcgctgc caggatatac gtaatctggc atttctgggg attgcttata
    17641 acaccctgtt acgtatagcc gaaatttcca ggatcagggt taaagatatc tcacgtactg
    17701 acggtgggag aatgttaatc catattggca gaacgaaaac gctggttagc accgcaggtg
    17761 tagagaaggc acttagcctg ggggtaacta aactggtcga gcgatggatt tccgtctctg
    17821 gtgtagctga tgatccgaat aactacctgt tttgccgggt cagaaaaaat ggtgttgccg
    17881 cgccatctgc caccagccag ctatcaactc gcgccctgga agggattttt gaagcaactc
    17941 atcgattgat ttacggcgct aaggatgact ctggtcagag atacctggcc tggtctggac
    18001 acagtgcccg tgtcggagcc gcgcgagata tggcccgcgc tggagtttca ataccggaga
    18061 tcatgcaagc tggtggctgg accaacgtaa atattgtcat gaactatatc cgtaacctgg
    18121 atagtgaaac aggggcaatg gtgcgcctgc tggaagatgg caattagcca ttaacacgta
    18181 aatgattgct ctaattcttt gatatttatg gtggcatatg agaaaggatt tcaacatcga
    18241 cggaaaatat gtagtgctgt ctgtaagcac taatattctg tcgccatctg tcattgtcac
    18301 cgtaaagttg agcgatagga tgcctgatat cgactcgata tctgttgcgt tccccgttaa
    18361 aagcatgcgg agtgctgaac attttgtgat gaatgcaacg gaggaggaag cgcggcgcgg
    18421 gcttactaga gtgatggggg aatttggcga actcctgggt aaggtaaaca atgccctttc
    18481 aatcagttca gcaaggtcca aagcgttaac agcttccatg atgaaataaa aaaaagcctg
    18541 gcaaggagcc aggctgcaca aaagagcggg tttgtattcc gcatccaatc aatcaagaag
    18601 gagtatagca cacaggtact gaagtgaaaa aatgtgattc gcgataaaca aaatatcgac
    18661 cattgctcta attgattgat ataattgaac cgcagttttt gtcaactacg aagacgttgc
    18721 cattacttca ctccttgaca tcattggcgg ccatcaggcc gccttttttt tgccatatga
    18781 aaacaatcga acaaaaactt gaacagcgcc gcgagtggca gaaggcagcc agagaacgag
    18841 cgatcgctcg gcaacgggaa aagtttgctg accccgcctg gcgagaatcg caatatcaga
    18901 aaatgcggga ttctatcgac cgccgtatcg ctaaacagaa agagcgccca ccagccagca
    18961 aaacgcggaa aagcgcggta aagataaaat ctcgtggctt gaaggggcga acaccgacag
    19021 cggaggaacg gagcatcgcc aatgctcttg gcgctctccc ctgcattgcc tgctacatgc
    19081 atggagtaat atctgaagag gtatctctgc accatatcgc cggtcgtacc gcgccgggtt
    19141 gtcacaaaaa gcaattgccc ctttgtagat ggcaccacca gcatgcagca ccggctgaag
    19201 taagagaaaa atacccctgg ctggtccctg ttcatgccga tggtgtggtt ggaggcaaga
    19261 aagaattcac cctgctgaac aagtcagaga tggagttact ggctgacgcc tatgagatgg
    19321 caaacatcat gcactaataa atatattatt tttaatgata aatgattgac aactgacaag
    19381 tgacttcagt cagaatcatc acatgcccgg tacggatgga tcccttttca aatattccat
    19441 ggacggcaca gtctgagtac cgggcgctac cttcagttgt attgctaagc cgccgctggt
    19501 ggcttttctt ttttgtaggg gcgttatgga taagaaaata tgcgttgttt cgatgagcgt
    19561 cggcaaaccg gcgtcaatga ctgctgcatg gatcaacaat gagctgataa tggctgagcg
    19621 gaccagctac cctgaacgcc gccgcgacat ggaactccag ctgctgcgcg aattgcgaga
    19681 aaaagaggaa aagggtttta tcgtgctggt ggaagaggaa aacagcttta ttactggtcg
    19741 agttggccag cgtgtaaggt tgcgagatcc ctttatgaac ggcaggccgg tactgattga
    19801 agcaatgcag atttacaagg aattggaacg ccagaaagcg atcaagttac cgcgcaaaga
    19861 atccggcaaa tacatcctcc accaaagcat cttcgattcc gaacacgaca aaaaaggcga
    19921 tgaatttttc aacatcaact ggagcgaaat aacgacagag catgttctga cgttactatg
    19981 ttgctttgcg acggaataca acaacgttgc cagcgcagac tacatcaggg caatggctgg
    20041 agaagttgag gcacgccagg aaccatcgtt actaagccct ctgattaaca taattcgcgg
    20101 aacccaaacg ctggcacaaa aacaggtgcc taagggagta ttaacaggaa aaggaaatta
    20161 tctataaacg tcaatgcatt agggtatact tctccgtaga actataagta aatggagtaa
    20221 gtaatgagcg agtatataga aattgcctat gcagcagcta cacataggct gtgctttctt
    20281 acaggcactg gattttcaaa ggctgtttct gatgataaag ccccaagttg gcaatcttta
    20341 ttggagcaac tgtgcggttt attgaaggat ggtgactcac tcaaagagga attatttcct
    20401 gatggaaaag caaaagacct tagccttgaa gaggctgctc aggttattgc actaaaattt
    20461 atactttcgg ggaaaaatat ttaccaggag attgagaaaa tcatagcctc aatcgaactt
    20521 gatccatcaa ttgaatatat tcaagacttt ttcaaagaaa acacatttaa agtaattacg
    20581 acaaattatg acaagttagc agaaaaactg gctggagaga atcgaacgtg tacaatcacc
    20641 cccggcctgc caattccaaa atataattgt gaggttaaag tctaccatgt tcacggctct
    20701 atagactccc catcagatat ggttgttact agcgaggatt atttcagatt tataaatggt
    20761 aattcttact tttcaaataa actaagcaca gttctacatg aaaacactat tgttattctt
    20821 ggctattctc taagtgacgc gaacctcaag gcgattataa atgaatataa ggtattttca
    20881 cgggacaacg taatgtcctc caatattttc cttatttccc gagggaaact attgcaacct
    20941 attaaagatt actatttttc ctgctttggg attagggtta tagataaaac agaggtgtct
    21001 gatttcttta gaaaacttaa caagaaaatt ccagaagcaa aaaaaataaa agacaaattg
    21061 aggcactcaa tcaaatcggt aataaaaaac ggaagagagt ataaaataga gttcttgaag
    21121 ttagaggatt ctttttatca tatcatttcc tccatttcat catctggata tagctggaat
    21181 gatgaaaagg tattaaatgt tttttgtgat ataatagata aaaaaataga tcttactaaa
    21241 gagtctggcg catgggagca gtatgaacac ctggcaaaat ggcttattta tttcggtagt
    21301 ttatttgaag taaaaggaac caactttgag aaaaaataca tacatgcggt tgaacactca
    21361 atgacttata tgaataaacc ttacgaaaca ggctactcat ggcgagcata tctagcctgg
    21421 aaaacaaaat ggccttcact gacagcatct aaccgctctc ttattaagag taagatggaa
    21481 gaaatcccgc tacaacagat tcacgatatt atatctaagt ttatatagta tatttatctc
    21541 cggcctaatc tccctaggcc ggagagttct taatcagcat tcaggagcaa tgcgttatct
    21601 atgatgatct gctcccattc ctcgaatgcc cgatcgcgga cgccctgggg aacgctgtta
    21661 gttttgaaat cgacgaccgt ccgccatttt ccgtcaggac ggtacatgcg cagagcttta
    21721 cttccccctt ccctgcgcac ctcaacgtta tgcttatcag caaactcttg caatgctcgt
    21781 agcgtcccat gctttactgt gtagtatcgc ttcttcaagt tttctctcca gcctgtgcca
    21841 aggcttcaac ttccaaatcg taagactcaa actcatagtc ctggtcgtca acctcttcag
    21901 gcactggcag taaatgccag gctgagtata tctgaccatt atcaaaacgc tcctggctgt
    21961 agagcgtcgc agctatgagc gtcagcgccg ggcggtcata acggtaaatt ttgcgaacgt
    22021 cacggtcaac gagacgaccg aaattaccat aaccgcgctc cagtaataat tttttaattt
    22081 ccggccagta tgggccatag ctgcggtaca ggcggggatt tttcagtaat cgcccgcgta
    22141 gccctgacag gaagaaatca acgtattcgt cttctgtctt tcctaacaac gctgtacgga
    22201 gtaccgcctc aagatatgtt ttattcggtt ttattgtatc agatagtgtg gccatattat
    22261 gcgacgcccg gcgaaccggg cgctcctgtt atgcgtattg ttggatgacg gccagaacgt
    22321 ccgccacgtt gtgttttgtc tcgataatcc accagttacc cgggaaatcg ctgttcttcg
    22381 ccttcgctgg cagccagcga gcgccgaatt tcgccttgat tgcgtctttc gcacggaaaa
    22441 gaacgccttt catgcctgag gcttcctgaa gcccaaatac ctcgccagcg gcgaattttg
    22501 gtgcgtacat catcttcagg tcggcggtgg atacgcgata attcagacca agagactgag
    22561 ctatgctggt ggcatcaccc tgtattgatg ataactcttc ttgtttctcg tttctggcgg
    22621 caatctcttc ctccgtgatg ttgccaaggg ccaggtttat ccgatcagcg tcggcctgtt
    22681 tctcttcatc ggtgcgcccg gcaagaaccg tgttaattct ctgcaatatc tccacatgat
    22741 tcttgcgcat gctgagcaat tccggcgtaa cctcgttaag atccaccagc ccaaggatgg
    22801 caaggtcgga aaacattgat accaggttgt aggtcatgcg atagctgagt tgaccatagg
    22861 ctgatggcaa ctgcaccgca tccattttat aggcatccat aaatttagag ccgtcgttta
    22921 cgacatccgc aattgccggt gtgatttttc ctgtggtggc ggcctccctg attgctgtta
    22981 cccacgattg agtcagcgcg gaaactgcat gattcagatt ggctttgcgt tctgctgcaa
    23041 tgcgcgcgct tgcagcgtcc attgactgtt tgatctcgtc tttattgctg taaatgccaa
    23101 tggtgccaaa ctgtgctgtg gtgatctcat aatctgacgc ccggaactca ttggtaccga
    23161 aaatggcatt ggtgacttca agttcagaat ccccgttacg agtagccccc tggcttgttt
    23221 tttccggcat tctggcgatc gcatccgcta ttttctcctg aattgcttca ggggatagcg
    23281 tatctccgta tgacgcgatt acatcgccat aattggagcc aaacagttca accaggaatg
    23341 tttctgccga acggatctgg cggttattcc cttccgacat cataccaagc acccattttg
    23401 caattgacga cttcagcgcg ccgtcacggc gatccgggta aaccgcatgc ttcagtgggt
    23461 ccgaataggt accaacaaaa tcgatgctat agcctgactc tgtagtctga acgccgtatg
    23521 agtcagtgat tttgatcatg ccgcgctgct ggaaacggta gaaatcgtca caggaaatga
    23581 tgtcgttaat cccggcgatg gagacgccac cactgatttt ctgcataaca gcatcttcat
    23641 cgggagttac atcaacctgt ttatccagcg tcttcacatc ccagttaccc gatttggtac
    23701 ctttgaaggt aaagataatc tccacgtctg cgcgctggct gtcgaagtcc agcgacttaa
    23761 tgcgaacgat atcaccggca caatcgtagt attggcctac acgccatgag cgatcgccga
    23821 taacaaggaa ctcactcgca tggttaacca ggtcaggatc aacatccaga atgcctttat
    23881 ttattgcatc ctccaccagc gggcgcaggc gtttgatatc cgtcgcggcc ttctgagtac
    23941 ggttcaataa tttctcatag cgggagatgg cttgagagat attagccttg cgctgaatgg
    24001 cgcttttcaa cgacgcgcga tactgtgcta acaacgtacg gtctgtgtga tggacgctac
    24061 cccagcgggc tttccagtct gcgttatcag ctgctttggc cattaccgcc tgtttgaatt
    24121 tagctacctc ggcggtggtc ttttcaagtt ccgctttgct tcgctctaat tcagcggtaa
    24181 gtacctccac atcctcgcca gctgcgtgct gcgccttgat gtagttatga aggtcgatag
    24241 tagcctgttc tttctggcga gcgcgttgcg cggctttcgc cttatccatt tgaacctgca
    24301 tcattgccag acgttcgcca tcatccttag cggtatacat ctgcatttcg atcatatcgt
    24361 tggcgtcggc gttctccatt tctgacttat ctgaacggag gatatcggag atccagcctg
    24421 ctttacgctt cagcgtcttc agtcggtatt catcgaaaga acccttgccg cagtagtagt
    24481 gaacgcgaac gcttgcacgg ttggagccaa ctcgggcacc gcgaccgtta cgctgtgcga
    24541 tactggctgg tgtccatggc aacgtcagat gatggatgtc agtcgttcct cgatgcaggt
    24601 tgatacccac ctctgccttt ttgttgcaga tgatgatcgg agtccggccc tcctggaagt
    24661 cggctgcaat cttttccaga ccgcccaacg acatttcatt ctgctgcgcg atataggcgt
    24721 catacagagc catttgctcg ttgtatttcg ctatctgtgc atctgttggt tcatccggta
    24781 actctttcgg cggtttaacc gctttcagtt tcttaccggt tttacctgcc tcggcaaccg
    24841 tctgagcatt caggatcccc acctttgaag gttcaaggtt aagagcattg cagataatgc
    24901 gcttgagctt ctggtgctgc gttttttcgt ccgtgaagat gatttgctta ccttccggga
    24961 aaaactcctt cagcgtggcg atcagcttca cgtatttggg cgtaacgggg tgagttacgg
    25021 tctgttcgtc aatgccaaac ttggccaggc gcttattcac ttcctgctcg aacgcttccg
    25081 gaacctgcaa ctgaataaac tcgcccttat ctatcaggga gtattgcgat tgctgcgtga
    25141 ttgaatcatc actgtcgtcg tcttcgctgg tggcttgttt aggcaaactg tccgccagct
    25201 gctgcaccgc atcggcgtac tccggcagga aacgataggt gatccggcga tagtacaggt
    25261 ccatgtcagt acatacgcgg tccatatccc tgattattga gaagatcgga cgggctttct
    25321 cgtgctcaat cacgccgtct tcattaaccg aggtcgttac gccattgttg gcttttgctg
    25381 ccgcttccgc ctgttgacgc aattcttcat acgccgccag ttgttcttca gtaagtggtg
    25441 catcctgctg gtgttcgtcc agttccggga tctccacggt atccttaacg tcttccgccg
    25501 ttttaagcgt tgtccagcga tggaatatgc cgcgcagcgc atcaaggttt tcaaagccca
    25561 ccagcgccat tttttcttca acttcaccgc tgattttctg taccgtttcc agcctggtct
    25621 tgccgaagaa tttaacgaag tcatcaggac cgtagatccc catcttctgc cagtattcct
    25681 tcggcagaac atgagaaagc atgttgtatg catcgatcgg ggtgttaacg actggcgttg
    25741 cagtcaggag aaccggtcca cgcccaccat tctttttcat caggtacgcg tttttgattg
    25801 ccatatctcg cgccgattgc gccaccgcgc tggtgggcag ataggccagc tgtgacgctt
    25861 cgcgaccatt tttatagcta ttgcggtagt tgtggccttc gtcagcaatc acgctgtcga
    25921 agcccatatc ctcaaagtac ggatacttct ctgctttttc ggtaccggta tctgaatact
    25981 ccgacaatac ccggcgacgc gccgcctctt tgcgatggga atcggagtcc attgcgctgg
    26041 ccacacgccc ggcagcaacg aagtcataaa gcatgtcttg tgcatgctca tctacggtgt
    26101 catcacgtag cggaatgcgg gcgtattgtt ctttggtaaa cacgactgca cggtagtttg
    26161 agtgcgggat cgcgttcatc cgcgccgtga tagtggcttc atctgccagc ttaagggcat
    26221 cgcgcataac gggagtgcca tctgcaccaa cgacaggttt accgttctca tcgagcaccg
    26281 gcacctggcg aatctgatcg ccatccatca gcacatcaag accgacgaac aggtagttac
    26341 tgaatgcctc ttcactcaga aactcttttg cttcgtaata ccagttttcc agcactgatt
    26401 taggcactac atacgcagta cgggtggaac gaccgttctc atagttgaac gcctcaagcg
    26461 ccagcgcggt cgtggtttta cccagcccgg taccgaagcc caggatgccg cgcccatctt
    26521 cggacagtcg gcgcacctcg ctattctggt aatcaaatgg ctgtcgctta ccgcttaatc
    26581 ccttcaaccc aagcggatcg ccagagtgtt catacgggat attgctattg aaaacatcgt
    26641 tgtatttggc aaccagctca tcgtagcgat cgtgcgtctt gatccactta ttgaactggt
    26701 cctcaagcag tgccatctgc tcgcggtagc cgttcgccgt cgcgctatct ttgccaccga
    26761 tacgcgcacc attgagatac ttttccagct gtgccgggaa cccggtcgcg ttttcacctg
    26821 atttacggtc ccactcgtag cggatctcgc ctgtttcttt atccttgcgc tggacgacac
    26881 cgtatcggtg cccgacgaac agaccatcac caccgtgata ggtgtcagaa accatttcgt
    26941 cgccttcaag ctgcactgac tgcacatagc gcagatccgg atagccgttt tcctgcaaaa
    27001 attccagaat gacggaacgg tcgaaccaac ggctattgag cttaaaccgg atattctctg
    27061 ctggcgtctt gatgcgcttc tcttcgatcg ctgccagctg attaaggacg ttgttcttta
    27121 ctggaccgtc ggggagcgtg gcgaggaatt cctgttttgg agccactatc tcgttaatgt
    27181 cgccgctggt ggcgcgggcg aacggaacaa tcccgccata cggtgaaacc gcaatgccag
    27241 gggtgctggc caataaatta agcaactcgt catcactggc tggcagttcg ccggtaaacg
    27301 caaggcggaa atcatcgagc tggattggat cgcgagtgag atcactgtag agataacgca
    27361 gggtgtcctg atagctggtg gagtcataac tggcgctgga atcatgcgta accagttttc
    27421 ctgtcagctc gtcagaaata gtgccatcca gcttaattgc accacggaaa gcaaaccagg
    27481 cgcgcgcacc gctccccgat aatttcgcta tcggaccgcg acctgggtta ccaaaacggt
    27541 caatctctgc ctgcaaacgg gataccagag aaaggcgctg ctgttcgatt tgttcagcac
    27601 tatgcccggc ggccttcatg tcctgatatt caattaacat ccggccaatc atcgccccgc
    27661 gatacaagcg ttcacggtat ttttcaggct ggctgttaat ccagtccacc agctgcacca
    27721 tatcgtcgct gattgatgtg gtgtacttat cgcggacatt tgccatctgg gtaaatgtca
    27781 tgccgagacg gccttctgtt gtagtcaggt tacgctgaag agcctcccag ctatccgcgc
    27841 cataactggc agcatcgatc ttaagttcct tcccggcatc agcttcaatc cagcgaccac
    27901 cagcatattt ttgccatacg ccattaatca ggcgcatttc cccttcacca acaacgtctg
    27961 cggtcggtga cggttcagcc atatcgagca aagaccagtc gatacggctt tcgaaacgat
    28021 gaatcagctt cgctttaaga gcctggttat caatctgacc gtcggcacga acctcaatac
    28081 gcccctggaa gcccttttcc tgggtgccat gaacaaaccg gcggccatcc ttttcaaacc
    28141 acttgccaga aataaacgtt ggccaaagca catttgccga ttcaagagtg ctttcatcca
    28201 ccagggggat tttctcagcc atctctgccg gatgtttgcg catcagcacc acatctacga
    28261 ccgtactggt cccgtttgcg tcaaaagtac cggtaggcag gcggtgggca ccaagaaatt
    28321 cagctttccg tgataggcgc aggcgtaacc gcttcatgtt tgaacctgaa acaatggacg
    28381 gcggcacaat cacgcacatg aatccgcctg gctttatttt gtccagcatg cggagcatga
    28441 agtaagaacc catgtccgtt tcttctgcgt aaggcttatc gatgttgcgt gtgttatcac
    28501 gaccgccgaa cggaacgtta cccacaacat ggtcgaatga atcgttaggc gtgcttacag
    28561 ccagctgttc gaacggggaa atctgtacgc tgtcttccgg gtgtaacaac tggtttatac
    28621 gaccggaaac actgctgatc tcagtcgcgg tcatcaccgt accaaccggt tttgtctcat
    28681 taaaaacgcc ggttcccgcc gatggttcca gagtgttacc tacgtccgcg ccgtagagct
    28741 tcatgatctc ccagacacct tcagcgatag gctttggtgt gtaatattcg gagacggacc
    28801 cgccaatgcc gccttcacca gtgtacccgg ccaggatctg gcgctgttca tctgtcagtg
    28861 tcgcgccgtc caccagcgaa ttaagcaaat ctatcgcctt ctgattcgcc tcccggcgca
    28921 gtcggtcata gcttttgcct tccacctttt ccacgccgta tttaatcggc gctcggtgag
    28981 atgttattgc cctaatgtat ttcaatattt cgctgacact tgaacagcga aacacaccca
    29041 tagatagctt gttcattggt aatccttaac aagtgactag tgttaaattc cgttcaaaca
    29101 cgatgcgaat tattctaatt aaggtgcaat cttggcagac aataaaatca cgctatcctc
    29161 ggtcaggaag gcgctggcgg gggtttttaa agacaacgga gaacgggaca acatcctcct
    29221 gtccgcgctg gctgtgcacg gcggaagtgg gtatttgttt tctcgcgcag gggcaccggt
    29281 acaactgtcc ggcttcttag gcggcaaacc gggcgatagt ggcatggctg gtgatgggct
    29341 ggtggatggg agtcgcttta tctttgatga agttcaactg ccggaagacc gcttgcaacg
    29401 ctatccgcta ctcgaagaaa tggcggttta cagcacgatc gccaccgcgc tgaacatcca
    29461 tattacgcac gcgctctctt tcgataagaa gaccggacaa accttctcta tcgtgccggt
    29521 acacaacgga aacgatagtg actatgacgc cgctcaggcg ttgtgtgacg agctgatgaa
    29581 cgacatcggg cgaaccatca acaaagaggt cgccggatgg gcatttatca tgtctgtatt
    29641 tggggtggct tatgtcaggc catacgccaa agaaggcata gggatcacgt cttttgagtg
    29701 ctcctattac acccttccga gcttcatcaa agagttcgag gtcagcggca acctggcggg
    29761 atttagcggc gattatctga aggacgcgtc agggaaaatg gttttcgccg atccgtgggc
    29821 cattatccct atgaaaatcc cctactggcg gcctaagtca aaccttatgc ctgtgcacac
    29881 tggccataaa gcatacagct tgctggataa tccggaagag cgcacgccga ttgaaaccca
    29941 gaattacggg accagcttgc tcgaatacgc ttacgagccg tacatgaatc tgcgttcagc
    30001 gatccgctcg ctgaaagcaa cgcgttttaa tgcgtcgaaa attgaccgaa tcatcggcct
    30061 ggcgatgaat agtctggatc cggttaaagc agccgattat tcgcgcacca ttactcagac
    30121 gcttaaacga gcagctgacc tgatggaaag acgcgcacgt ggcgcgaata acatgcctac
    30181 ggtgaccaat accctgctgc ctattatggg cgacggcaag ggacagatga ctattgatac
    30241 tcagaccatc caggctgaca tcaacggcat tgaagacatt ctcacctata tgcgccagct
    30301 ggcggcagca cttggcctcg attacaccct cctggggtgg gcagatcaaa tgtccggcgg
    30361 gcttggcgaa ggtggattcc tgcgcacggc aattcaggcc gccatgcgcg cctcatggat
    30421 ccagcagggc gtagaagagt tcattcagcg ggctatcgat attcatcttg ctttcaagta
    30481 cggcaaggta tacccggaag gtgatcgccc gtacaaaatc gaattccact ccgttaatac
    30541 cgctctgcaa caagagcaca acgataaccg cgactcgcag gcgaactacg ccactatcgt
    30601 tacgcaaatc ctcgatgccg tcagcaataa cagcgtcctc gccaattccg atgcattcaa
    30661 acgttacctg ttcagcgatg tgctggagat tgacgaaaaa atctctgaag cactggtgaa
    30721 cgaactgaaa gcgaaaagcg aggacgacga tcacctgatg gattccatca tcaaaacacc
    30781 gccacaggaa ctggcgcaaa tccttgaatc ggtctttaaa gagggaaacg ataatgactg
    30841 atgttttgaa aacggtcact gaccgctttt gtctctatag caatgctcga aaaggtcgcc
    30901 agaacgggcg acagtatgta ttaagcgcgg tcaagaccat gcttgaaagc aaggaaactc
    30961 aggaaggttt acgccttgga gagcttttcg gctattacgg tcacggtcgc cgacagctga
    31021 ctggcaaact ggaagtacca gaaaccagcg tgatcatggt ggaaggtcgc ccggtcgtaa
    31081 tcgacaatgt tccagcgtgc cgcacagtgg ctatatctgt tgacgacaac ggcatcgtta
    31141 cccatacaca ggaaattctt aacacagagc cgggtaaaat tgtcgccgcg atgatcgaaa
    31201 gccgagctgg tggctggagc tgggccactg gcgggcgcga gtccgggaaa atcgctgtaa
    31261 ccaccagctt ccatggtgtg gattatgtga caacgccgaa ctatatcagt ctggatcatc
    31321 ctgccagcgc cggaatgttt gaaagcgcgg attctaaatc tttactggca gagtccctgg
    31381 cggcgcatgg gtactccgac gagtcagtgc aggccgttat atcccattac ggcaaaatgg
    31441 ctgaactgga aatgatggtg gaggcgacag agcgtacggc agaactggaa actgcactac
    31501 tcgaaagcca gggccgccac ctcgaagcaa tggccaagat cgcagatgct gaagcgcgaa
    31561 tcgctttgct ggagaaaaca gcgggtatcc gcgacgatgt gctggcagca atgcaagacg
    31621 aactggataa cctcccgatc ttcgtctccg ccgcccaaaa agacgcattc cgcctcaaag
    31681 aacctggtga tgcaaaaatc gttgccacac ttttcgaatc tctgatcaaa gttggcgcac
    31741 gcaacttgcc tgtcaccaag aaaattaagg aggttccgca agcggctaac gtccaggcac
    31801 cgcgtgagac aagcatcatc acgtttaata attcaatcaa tccgtttaaa taaccaccaa
    31861 aaataacccc ggcggctgcc ggggttatcg ttaactatta tcgccttcgc ctgcgtgcca
    31921 tatatttgcg caccgcgcgg cgtggacaat ctgaagcggt ttctttctgc tgcatcaatc
    31981 ttgcagccat gctcaaaaat gtcaggcaca gtcgaagccc agcatacaat agcggttcca
    32041 gtggccacgt ctcattgagc acatataccg ccatgaaaat cgagtcgaaa actatcgccg
    32101 ccagcgataa cttcattgtc gaaagtcggc ggagctgccg gagtttattc attgaccagt
    32161 cccgtcaggc aaagctggcg ttctttttca cggcgaatct ttaaacctcg caggggcacg
    32221 ccgttactgt tcacgaaatc agggagatgg ttacacatat tcacccattc ccctttctgc
    32281 gcccacttgt ggatggacgt ttctactcgc atgcctcgcg ctttgctgta gtaggtccgt
    32341 aagctattgc atcccatatt gaatgccgcg cttgtcattg cactgaaggc attatcgggc
    32401 atgtctttgc cccggaagtg ctgattaata cagcgttcag cgatcaggat attcttttcc
    32461 caatcagcgg cgatttgctg gtcgtttttt cgcacacccg gcgttacccc gtgtgtatta
    32521 ccgatcccgt cagtccatac ccccgccggg cacatgtatg gatcacgtcg gcaaccttca
    32581 gcgttaccaa tcagctcaag ccccgcctgg ttggttcgca cattgccatt acccatcacg
    32641 atggtaatca tcaccgcgat agcgcaaatt gcaccgcctc ctgcggctgt ttttcccttc
    32701 ataaagacct cataagcgaa ttttttacgc tccaggacaa acactcattc acagccaata
    32761 ccgactgact cgatcccttt agaaggcaca ggataatgca aatcacttgt tagctacgtt
    32821 tcaaagatat acattattgc tctaattaat ttattttatt aggtgagata agtggcacaa
    32881 cgcggtgtaa acaaagtcat cctgattggt accctggggc aagacccgga gatcaggtat
    32941 ataccaaatg gcggagcggt cggaagactc agcatcgcaa cgaatgaatc atggcgcgac
    33001 aagcaagcgg gccaacagaa agagcaaaca gaatggcata aagtcgtttt gttcggaaaa
    33061 cttgctgaaa ttgcgagtga gtatttacga aaaggttctc aggtctacat cgaagggaaa
    33121 cttaaaaccc gcaagtggac agatgacgcc ggtgtagaac gttacacgac ggaaattatc
    33181 gtcagccagg gcggcaccat gcaaatgatc ggcgctcgcc gtgacgattc acagtcctca
    33241 aatggctggg ggcaatcaaa ccaacctcaa aaccaccagc aatacagtgg tggcggtaaa
    33301 cctcagagca acgccaataa cgaacctcca atggactttg aagacgatat tccattttga
    33361 gagggctgaa agttgctcga ttacttaaaa ataactttac ctaattggtg taaaaaaatg
    33421 ggcgcaacaa agcgccccaa caatagagtt tcaaattaat aaaagggtaa taaataacat
    33481 gaagttaaat tgagaagagg caattttatt gcctcttcaa aggtggcatg agggtgccac
    33541 ctatatgcgg gcagtgacat cactcccttc ccgcatattc ttacctacca aaattagaat
    33601 tggtaggtca taccaacagc aacgatgttg tcggttgcaa cctcagatgc tttggtaaat
    33661 tcactttcat caatcaggtt gatcttgtag tcaacgaaag cggacatatt tttgttgaag
    33721 taataagtcg cgcctacgtc gatgtattca actagatcct ggttaccgaa cgcaccaata
    33781 ttttcaccgc gagagtgcag gtaagcgatg gacggacgca ggccgaaatc aaactggtat
    33841 tgagcaacag cttcaaagtt tttcgctttg tttgcgataa agtcatcacc gaagactgtc
    33901 atattctgag tttcagaata agttgcagcc aggtaaatgt tgtttgcgtc atatttcaga
    33961 ccagtagccc atacttcagc tgttttacca gaagcattca gagagcttgc attagcatag
    34021 gatacctgtc catcagtacg gtcagatttt gcataagttg cgccaatgcc aaatccttca
    34081 taatcataag tagctgacag accgaagcca tcaccattag attcaactac gtcgcggcct
    34141 ttccagttgt tcacttttgc agcactatca tttttaccct gatactgcaa agcaaagttc
    34201 agaccatcaa ccaagccgaa gaaatcattg ttacgatagg tcgcgacgcc agtagcacgc
    34261 tgggtcataa atacatcagt ctgagtccag gtatctccac caaactccgg cagcacgtca
    34321 gtccaagcgc caacatcata cgctacaccg tagttgcgac catagtcgag cgaaccataa
    34381 tcgccaaagc gaagccctgc aaacgcaaga cgagttttgt ctttatcaga accttcagtt
    34441 tcagtacggt tacctttaaa ttcgtactcc cactgaccaa aaccggttaa ctggtcatta
    34501 atctgagttt cacctttgaa acccatacga gcataagttt tatccccgtc atcactagct
    34561 gaagaagaga agtaatgctc tgcattaact ttgccgtaca gatccagttt gttaccgtct
    34621 ttgttataga tttctgccgc ttgtgcagac attgccatca ggactgatgc tgcaaccgca
    34681 gaaagtgcca ctgtgatttt tttcattttt ttgcccttta gattgaactt ttcagtagaa
    34741 aagaggtcac tgcggacaaa tgtttatctt ttttggattc gtgtttcaag ttttgcaaat
    34801 aaaaatcaag gtatttttgt gatcaaaatc acaaataata accttgaaaa cccaatggac
    34861 tattagaaaa atctcaattc acagaataaa aattgacaaa atagaacaaa acacaatcaa
    34921 caataagcac aaaagacaaa caattaaatt agcacaaaaa taataactgc aaaaatagat
    34981 agtctattaa ttaatagggt tttaatggat gtggatttga aatctactta aaaagtagag
    35041 tcaaattaat tactgtcgat tgggtattaa actgaatcta tattgtgaga gttaaagacg
    35101 atgtgtttta gttatacatg ttattcccta aaatggagta gtctcatcga attctgatta
    35161 ataacgagct aagccaattc attttagtat gtgtatatct ttaccctctg aatatgcaat
    35221 gacgaagaga tattaaacaa tatgtagctt caggattgac tctggaagaa ttaaaagatc
    35281 ggctaaagtg ttcaatatgt ggtgagcgaa acgcaaaaat taaaattttt tgatctaaca
    35341 tattaggcaa tagcttgctt ccataaccta aagcaagcca ctacgcgttt actttaagta
    35401 cacaattaac atatcagaac agattatttg tgcactatca gggagttaat atacgatctg
    35461 gtctacatga tccccaaaat catcgtcgtc ctcatcgcca ccctctactg ctggccaatc
    35521 gacaaaccag ccagcgtaaa gatgcagcgt tcggagaaca tcacttgcgg gagcatcaag
    35581 ggtgttaacg aatcccatat agctattggg atttgcccca gctatggctt cagcgatcat
    35641 gtcctcggta atgtcaccgg agataatgct taaacgcccg gaaacttctt cattatcatc
    35701 aaattcgata atggcatctc cgcctaatgg cgctgcgatt ttaatctgca ttatttagct
    35761 cctttgccac acctaataac agttccagca atccgtcacc attcatcagt gatgcggcag
    35821 cggcctcttt gtcatgatac aactgaagag ccatagagaa tacttccgtt gctgatgttt
    35881 ttgaaatagt cggtgctttc tgccgaattt tcccggagtt actcactgag gctggcgggt
    35941 ataccttcgc catataaata ttactcaatc gagatctgaa gcaccattca ggcttgccac
    36001 gcccaccgat attaacgaaa gatggcttat ccccttcaac attggccttc aggaatgacc
    36061 gggctttctc taacaaacca gggttactgt actcaagatg atgacccagc tcgtgccaca
    36121 gtgcacttgc attttcatcg ttcaaattga cagcaacaac accattaaga tttgcatatg
    36181 cccttccctg gtggtgaacc acctttgata aggtcgaaat tttaccgccg gtcaggcgat
    36241 aaatatcagc aagttccttg cgcaggtcta tcccaccatt ctgtccagcg cgggcttctt
    36301 ccacttcttc cgtgataaaa gagtcggccc actcaagagc tttttcttca gatacggatg
    36361 agtttgcgat cgcactgttc atggcagata acactttctc gtggaccgaa cccatacttc
    36421 gctgattcat ttgccagcgt gtctgcgggt tatatgagaa tcgcttaagt agttggtcaa
    36481 gctgctcaag ttcttcttca ctgacatacc ttttagcctc accaataatg ccagggagaa
    36541 tattgccgtt aggattaaac gctcgcgaaa ggaagagttt cagcgccccc atgccctccg
    36601 atgcttcaat atcaccaata acccggttaa caatggccgc actcttcgga ttagcatccg
    36661 ccaacgctct ggctacgatt tgcagggacg atacgacctc acgctgcata tcagtcctga
    36721 tctcatcaat aaactctggc gttatgccgt gctctttaag gatatcccgg ccttccgccg
    36781 ttaccccatc gatatcaccg acatgtttat taacacgact ttgcaatgcc ttaaatgcct
    36841 tcagaattcc acgggcatca tccgctttac taacggcctt cctgaatgct ggcaagaagt
    36901 ctgagttaac ctcattttgt tgatcggccc actgaatgga ggcttctttc atctcgtcca
    36961 gagtcagatc acccaacgcg gtatggtctg tgaatatgag cgacaacctc tgaaccattt
    37021 ctgccaatgg tgatgccgaa tgcgccgcgc taaggaatgc tttcaccctg gttgggcgaa
    37081 tggaaaacca gtcaatagct ggtggcatat ctccgttttt tatcgcctgc gctatctcgt
    37141 caaagccatc gcgcccaagg gaggatgcgt gatttaacaa gccgcgaagt aacgaattgc
    37201 tgataccgaa taatcggcac cattttttca cgtcggcaac aggcattcga acaaaatgcg
    37261 caagcacttg tacaagctgt tcatcctggg gatctgtgcg ggaaagcagc ctgatcagat
    37321 gaataatgtc tttgatgccg gatgcccgat gtaatagcaa actggtatac ggagcaacac
    37381 cgttgtaact accgccggaa acggactcga aaagaccgcc ggatatccct tgcatgcctt
    37441 cgttttccag ttcctgagac acctggcgaa ggatatcctg taacgacaca tcgccgccgc
    37501 caaacatatc ccccagcgcc tggccctggt gctgtaactc atcattgata cgttgagcca
    37561 tcaacttaaa ggcggtggcc atacgcttcg cgctacggtt attcgcgacg atgaacaacg
    37621 cgagtgcttt cacttccggg gccgtttcgc tgaacatatc cccctgagca ataacatcgg
    37681 taatatgctg gcctgactcc ttcgattgcc ttaccaggtc taccgcatct ttcaatgccg
    37741 ccagcgcctt tttatcgagg ctatccgctg tctcaatgcc atcaacaata gttgtcacag
    37801 cctgcttgtg cgcttctcct gataaagcct gcatctggac aaaatcattg gctgccgcat
    37861 taagcgccgt cagaacatta cgcatatccg gatcaggttc ttctgcaacc atccttacca
    37921 ggcgcgcatc cttatatgcc ttggcaaaga tcgcgttttg tatacggtct acaagttgcc
    37981 gtgttggtcg cccatcttca gttacaaggc cagccgcctg tgtggcacca acttgcgtca
    38041 taaatccgcg aataaacgcg tcattactgc ggctaagcag atctccgctt tctgacgggt
    38101 taaaaagcgc catcatcgcc ggtgttatgc tgtcggcatc aacaaaagcc ttttcactgg
    38161 ctgccatttc ctgaagatca gaaatatttg agtccttggc aaactgaacg cggtcaacct
    38221 tagttaaccg gcggcgcacc agtaccggag ccgtcattga ttcaaccttt tcaggtcgta
    38281 tgccgaattc ggtcgcatga tcaatcaggt actcacgata ccgatccgca ttgccgtcct
    38341 gataggcttt gatgatcccc atggtccgtc cattacctga ctcaacggca ttgtcctcac
    38401 caattatcgg cgcaccatgg ctggataaac cggaatcagt aagctgagca ggccgcaaat
    38461 ctttggatat ctggttaacc tgaagaaggc tggatgcgcg ggtccggtcg cgcggctgaa
    38521 gttcctgggg atagtcagga ttaattttcc catccagagt attggatacc aaaagagctg
    38581 aggcatcgac gatatcaaac gctgttttta cctcgtctcc cttcgctgtc accacatacg
    38641 aaacccgccc ataatcgggc aggttcttaa gcagctcgat cagcgtttct atgctggtgg
    38701 ccattaccac ctgatcgctt aagctcatcc ctgttacgcc ttatgctgcc tcttcaatgt
    38761 tggcggctat ccatgccgcc gtgtgctgtt taacctggtc caggtcgatg tatgtgccaa
    38821 catattgact caagtcctgc aaggtaccga taaatgcatc tgtgctctga tcgacgaatt
    38881 tatcagccag gaaatcagca accagttttg gcacaccatc atgtaccgaa ggttgttttt
    38941 cctcgccact actgccgccg gacgcgccgt accccatctg ttgcatgatc tggtcaattt
    39001 catcgctgat atccagcaac tccatgccac tcgcggtagc cgctttggac atcagagcat
    39061 ccaacttatc gctgagatcc attaactcaa tagctgatag tgtcatgccg ctacccccgc
    39121 tttctggatt gctaccaaca gatcagccag gtggcgagct gcgccgttaa ccagctcttc
    39181 gttttcctca aaacgtccgg cagcctgaag ggctgcaatc gcttcccgga cattgccccg
    39241 ggcgttacgg atctccgcca tgtcagtgct ttgtatatcc atcacgttat tgagatattc
    39301 aatggcttta ttagcctctg catctgcttc gctaaccgtt tcatcaggct gtgccggggc
    39361 cggttctggc tgagtaatct caccgacttc ggcctgcaat gcattgatca tgctctgcac
    39421 cattttctcg gtgccagcgc ccccaggaaa cgcaatattg gggaaagttt tttgaaactg
    39481 agttttcagc attacgcgga actcgtctgg tgagctggtg gccagctcca gagctttttg
    39541 tgcatatttg ccaaaaggac cattagtaag tgtcttcgcc aggaagtcga aagaatcctc
    39601 gcgaggcaat aacttcaggt cgtactcact catttgctga tcagaaagcg gggtatcgta
    39661 agtagcaatg ccgtagcgtg catattcata atacgggtca ccttcatcag ggcgcggcag
    39721 aatcgctttg ttaccttcag gtattgcgcc aggggccgcc ggacgcattt gcagggcata
    39781 tcgatatgca cctacagaga cttctggttc aggcgaagag ctaccggtat cctccgctgg
    39841 ttcaggttcg acgttttccg gtttatgttc ttctggttgg accagatatt ccgatacatt
    39901 acccgcttta taggctttaa acagcttgcc gatcgcatct gccatgtcca caccctgtat
    39961 ggatttagcc ttgatcatgt atacgctgcc atccggatcg gttaactgga tatacccttc
    40021 gccatcccca atgaattgct tcattgatgc accattactg agcgtcgctt ccccgtccat
    40081 atgcatacga tttttgatac tggcaaggcg atccgtcagc gcgcgagagt gcccaccagt
    40141 gcgattgagc tgatcaatct ccgtctgcaa acgctcattc tcttcataaa gagaatccgc
    40201 ttccgatgca acagcgttaa ttttctgctc cagatctgcc ttctgccctt ctaccgctgc
    40261 cacctgatcc gcgaggtcgc tcatggcatc ctctttctgg tcactgtcag cctgtagttg
    40321 ggttatttca tcaacaaggg cttttttctt cttctgcgca cgctggaatt ttgacgagtt
    40381 tttctctgca aggttggcaa gtttcatggt gacctgcgcc agcgtcatat cacgtccact
    40441 catcggagca acggtgtgag taacgtcttt tttattcagt aagaactgga aagcaaccag
    40501 cgtatcgcta ttggtgatcc ggttttccgc tgtcgggcta tgaaacaaaa tgctgatagt
    40561 ctgaccatca ctgagcggaa taatggctgg caggaccggc agcccgttaa cattacgtgc
    40621 ccggccaatt tcagcgccgc cgatcgcgcg cgcgccgctc tgggccacat cccccgtttt
    40681 atcactcccc gcagagattc cggtaccatt cagcttctgg ttcaatgccc ggacaaatgc
    40741 ctgcatggtc cggtgtaact gcaaacgagt agaactaatc gcctccagta aatccgtagc
    40801 acaccagtgg atcggcgtgt catagaagaa cgtagcctcg atttcctcca gggtgttgga
    40861 ttccgtcatc agatagcggt cctcaccggc cattaatgcg cgatattcat catcagtcac
    40921 tggcggggga agcacgtcaa gcccaggttt gatcgtcacc cctttattga tattgaactg
    40981 ttccatgtta atttcctgct ttcagttgct taagacggcg tttgagttcg ccatttcggg
    41041 ccttttcgtt attgagtcgg cctgtctcct tatccagctt cgcccgcaaa tcagtgatct
    41101 gctgttgagt gaaagacacc gagttctgcg ctgatttata agcggcaacc acctgagcat
    41161 tccgctgttt tgcctcttgc aggcgctgaa agttggattt tactgccggt ttcttgtcta
    41221 ccggattggc aacacgtttc gctttggcga tcagtgattt ctggaatttt gcggagtttt
    41281 tgcgggccgc ttgccccatg acggtaccaa gcgtcttgat atccggcgac tgagcgttag
    41341 gaatagcttt tccattcagc ttcacagacg atatatcgcc agtatcgttt acctgtatgg
    41401 caagaatttg tccgtcgtta agaaccagct ttgcggtttt aactttaacg ccatctttcg
    41461 ttgttgcgcg gttgctggag tcaacctcaa ttaccgtaac accggtttta ttgatcgccg
    41521 cgataaggga tttcagcccc ttttcattaa cctggtcaaa atcgaccgtt gcatacttat
    41581 ttttcgtcat ctgacacatc ctgtgcgaga tttattacgt aacttctgcg gatttgctga
    41641 gtaacaggga aaatccgata caacgggtta atgaacgagt cgccatgcgt aaccatgacg
    41701 ttgaaatgcc acagccgttc tcctttaccc atatattcag tgggtatgta caaccattca
    41761 ctgttttcgc cctgttcagc cgacgtcaga caacgttgtt cgccttcaat cactgtcgtt
    41821 ggcttctgaa catcgcggat ccaatatctg accgttgcgc cgcgcaaaaa cgggaattta
    41881 gaccggtatt tgaacggcac ccggatgaaa cccggtttaa tttccacatc accaagttct
    41941 aaatgcgtga tgtccttgcg ttttagcaaa tagcgatcgg ctaaggctaa cgcaagaacg
    42001 catacacccc agccaatcat ttcccgcctc cctttttcac caaacttgta agaacattca
    42061 gaatgctatc gatattcact cgtttcatcc ctgaaatcac ctcatgaccg ttattgctgg
    42121 ctatcgttac cattaagtac gtaattgata actcccagcc ctcgtgttgc cccaataggt
    42181 acgccaccgc gccagctgtc actgcaacaa agatctccgt aaccaatccc aacaaattgc
    42241 cagactggcg accgtctcgg acatccatca ggaacgtgcc tatcccacca attactgaaa
    42301 gcaggagcgc aatagcaact ggagctaatt cctgtgtgtc aagcacaagt tccctcctac
    42361 gttgtcagga ggtaatggta tgcaaagtaa cttctcatct ggttgttcat aatttgccaa
    42421 tacattcgta aatcaagtga aaaacccata ttttggttat tcttaataca aaatggctat
    42481 tccaactaca tatcaaaaaa gcaaccacat ccaaaaatga gtttccccac tgaacttctg
    42541 caagacaaaa accaacaaaa ccacctttta tggcaagcag ccatgcttca agccaatgta
    42601 aaaaacatgg cttataagct ccacgcctaa ctccagtaag aaataaatag gttccactaa
    42661 taatggacat taaaaaaatc aaaatctgca taagctcctc ccggatctaa caaaaatcaa
    42721 gcaggaacct attcagattg ttatttctgt gcataacgct ttgttcttta aaattcgcag
    42781 actcataaga acaacgcatt agagtaaaaa tcatgctgat cggctatgta cgcgtatcaa
    42841 caaatgaaca aaacactgct ttgcaacgaa atgcccttga aagcgcagga tgtgagctaa
    42901 tttttgagga taaggcgagc ggcaaacaga ctgaacgccc tgggctaaaa aaggttttgc
    42961 gtatgctttc cagaggtgac accctggtcg tatggaagtt agatcgtctt gggcgcagca
    43021 tgcgtcactt ggttgtgctg gtggaagagc tgcgtgacag aggaattaat ttccggagtc
    43081 tcactgactc tatcgacacc agtacaccaa tggggcgctt tttctttcac gtaatgggag
    43141 cgctggcaga aatggaacgt gagcttatcg ttgaacgtac acgcgctgga cttgatgcag
    43201 ctcgcgcaga aggtcgtata ggtgggcgtc ggcctaaata ccaagaagaa acatggcagc
    43261 aaatgcggcg attgctggag aatggcatcc cccgtaagca ggttgcaatc atctatgatg
    43321 tggctgtttc cacgctttat aagaagtttc cggcgtcgtc atttcaatcc taaaccttgg
    43381 tttaagagaa ctcggtacca gcggtgaaaa gatccccctg ttgagcacgg ctaacacatg
    43441 gagtgcgcgc cagattttca acggagggat caccggggca ctgacaggga acgccgacac
    43501 cgcgacgaaa ttgaaaacag cacgcactat tggcggtgtg gcatttgatg gtactgcgaa
    43561 tattaattta cctggtgtta acgttgcggg taatcagaat acatccggta acgcggctac
    43621 agcgaccaag ttgcagacag catgtactat caacggcgtc tcgtttgatg gttctaaaaa
    43681 tattgagcta acggcggaag atttaaatct acaggaattt attaataaag caaataatgc
    43741 cgttcagcgt tcaggcgata tcttgtccgg cggacttact tttgaaaacg actcaatcct
    43801 tgcctggatt cgaaatactg actgggcaaa gattggattt aaaaatgatg ccgacagcga
    43861 cacagattca tacatgtggt ttgaaacagg cgacaacggc aatgaatatt tcaaatggag
    43921 aagcaaacaa agcaccacaa caaaagacct gatgaatctt aaatgggatg ctttgtctgt
    43981 tcttgttaaa gcccttttca gcagtgaagt aaaaatatcg acagtcaatg cactgaggat
    44041 atttaattca tcttttggtg ccatttttcg ccgttctgaa gaatgcctgc atatcatccc
    44101 tacacgagag aatgaggggg aaaatggtga tatagggcca ctacgtccct ttacgcttaa
    44161 tctcagaact ggccgcataa ctatggggca tggtctggat gttacaggag atataacaac
    44221 taacgcctgg gtgtatgcaa acaggtttgc tattaatagt ggctcaacct catggattga
    44281 tatgcgaaac cagaatgtca tttttggtag aaacgcagta tcaacaagtt ctgcgcaggc
    44341 tttgttaaga caagaccatg cagaacgcaa attttttgtc ggtggacttg gtaactacca
    44401 gtttggtttt tacatgatta ataactcaag gacagccaat ggcaccgatg gtcaggcgta
    44461 catggataat aacggtaact ggctttgtgg ctcgcaagtt attcctggca actatggcaa
    44521 ttttgattcc agatatgtga gagatgttcg cctgggtacg cgagttgttc aattgatggc
    44581 gcgtggtggt cgttatgaaa aagccggaca cgcaattacc ggattaagaa tcattggtga
    44641 agtagatggc gatgatgaag ccatcttcag gccaatacaa aaatacatca atggcacatg
    44701 gtataacgtc gcacaggtgt aaatgatgca gcatttaaaa aatattaagt ctggaaatcc
    44761 taaaacgaaa gaacaatatc agctaacaaa gaattttgat gttatctggt tatggtccga
    44821 agacgataaa aactggtatg aggaagtgaa aaactttcag ccagacacaa taaagattgt
    44881 ttacgatgca aataatatta ttgtcgccat cactaaagat gcctccacgc ttaaccctga
    44941 aggttttagc gtcgttgagg ttcctgatat tacagccaac cgccgcgctg atgattcagg
    45001 aaagtggatg tttaaggatg gagctgtagt taaacggatt tatacggcag acgaacagca
    45061 acaacaggcc gaatcacaaa aggccgcatt gctttccgaa gctgaatcag tcatccagcc
    45121 gctggaacgc gctgtcaggc tgaatatggc aacagacgag gaacgcacac gactggaagc
    45181 atgggaacgc tacagtgttc tggtcagccg tgtggatacg gcaaatcctg aatggccaca
    45241 aaagcctgaa taaaaattaa ggcccgctat cgggccttgt ctcattcagg ttgttcggga
    45301 aatgttactg gcaggctgga ggtgtctgta gattcgactt tctgcgcata gagcatccac
    45361 tcagttaatt tttgtttatt ctcgtcggaa atgatgccca gccgtagctg tgagtcccat
    45421 agctgggttt tatccctgac aagttgcaac aggctttgtt tttcattctc tgcctgctgc
    45481 ctttgttcct cctcggtata agttcgcttt atcactacgc catctttgaa catccattta
    45541 cccgaaatat cagcccggcg atttgctgta atatcaggaa cctcaacgac gcttgcgcct
    45601 tctggattaa ttgctgaaac atccttttca atacaaataa taacgtcgtt gtggtcatag
    45661 accattttca acgtatcagg ctgaaagttc ttttgttcct cataccagtt tttcccatcc
    45721 tctgtataaa gccatttgat gttaaattgt ttcgttagct ggtattgctc ttttgtttta
    45781 gggttgccag cagtaatgtt ttttaagtgc atcatcgtta aatactcccc gcgttatacc
    45841 acgtcccatt aatgcaatac tgaattggcc ttgcctgagt tgtatcaatt aattcatcac
    45901 ggtttccgtt aactgaacct gtaacgacat aacctgacct gtcagaccag ccgggaccat
    45961 tccatgtctg aacagatgac agaccgccca ggcgaatacc tgtaataaac cttgagttac
    46021 attctgcctg cgtatatgca ccaacatccc ccgcagaggg tttgcgggtt gtggtgtaaa
    46081 actctgacca gttagcttca aagccataac catcacgcgc tgaacgataa aagataccgc
    46141 cgttcttata attcacgcgg aactgtacag cagggcaact ccccgcattc atattgaagt
    46201 ggaggattaa tgtcgatgcg ccactgatat ctgcatcata aacgccgcta ttccagttcc
    46261 agccaacagc tttatcattt gcaaccctgc ttcctgtttg ccctaaagca aatgcaggct
    46321 gctggttttt cgtgttgtag tctcttcgcc agccaggagc ataagcatca ccatgattaa
    46381 tataagtgaa ttgagcgtta gtaattccgc caccgctgga cgtgctcggc gtagtaacgc
    46441 gtatggtcat tgcgccgcga gtgccaataa cttccaccac agcacctgca agacaaatat
    46501 ttccgcaacc tgtatctgta atgaccttat tatttgcata agcccatgag cctttgcaca
    46561 tccagtaagg atggttaaat gctccctgac tctccagcca cgaaataaat tgcgcggttg
    46621 tccagacctg actatcgcca ccaatattca gccatgcgct atatgcgcgg caggcaccaa
    46681 tatttttggt gaaggtatct tttcccggaa tatctgcgcc gttctggttt ttctgtaatg
    46741 ctcctgcggc taattctaca gtttgctcaa gatttaaatc tgccgccgtt agctcaatat
    46801 ttttagaacc gtcaaacgag acaccgttga tagtacacgc cgtctgcaat ttggtcgctg
    46861 tagccgcatt accagaggtg tcctgattcc ctttggtatt aacgccggga acggaatctt
    46921 ttgacgtata gatctgtgcc cattcggacc atgccgcagc agtgctatct cttttggatc
    46981 gaatgaaagc tggtgcatga gcgccatttg tgccactcca gccaatgagc aactcgcctt
    47041 caccagtgga tgcagctccc ttcaggtgaa gtacattccc gtaagtcgtc ggataaccat
    47101 tgttgtatgc ctcatacatc tgcaatccag tagcagcacc ttgcgttgta ccggtaagcg
    47161 cagtaacgcg tcctctagac gtaattgttg gtatcgaaat gttcgtagaa ccatcgaatc
    47221 tgacgccatt aatgtttatg gctgttttta atttcgtcgc ggtgtcggcg ttccctgtca
    47281 gcgccccggt gatcccgccg ttaaaagtct ggcgtgcact ccatgtgtta gccgtgctca
    47341 a
//
LOCUS       DAMZET010000013        47204 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.013, whole genome shotgun sequence.
ACCESSION   DAMZET010000013 DAMZET010000000
VERSION     DAMZET010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.013"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(109..1194)
                     /locus_tag="POB34_000888"
     CDS             complement(109..1194)
                     /locus_tag="POB34_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDI5917880.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(1206..2510)
                     /locus_tag="POB34_000889"
     CDS             complement(1206..2510)
                     /locus_tag="POB34_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HDI5917881.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(2522..2875)
                     /locus_tag="POB34_000890"
     CDS             complement(2522..2875)
                     /locus_tag="POB34_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HDI5917882.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(2886..3764)
                     /locus_tag="POB34_000891"
     CDS             complement(2886..3764)
                     /locus_tag="POB34_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HDI5917883.1"
                     /translation="MSFDPTGYTLVHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(3761..4987)
                     /locus_tag="POB34_000892"
     CDS             complement(3761..4987)
                     /locus_tag="POB34_000892"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDI5917884.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFCDVIDRVEQALVSAGWQVERRGDELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMIATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(4987..6150)
                     /locus_tag="POB34_000893"
     CDS             complement(4987..6150)
                     /locus_tag="POB34_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HDI5917885.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            complement(6234..6596)
                     /locus_tag="POB34_000894"
     CDS             complement(6234..6596)
                     /locus_tag="POB34_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDI5917886.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AITHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(6613..7518)
                     /locus_tag="POB34_000895"
     CDS             complement(6613..7518)
                     /locus_tag="POB34_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917887.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGGSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRADDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(7787..8791)
                     /gene="trpS"
                     /locus_tag="POB34_000896"
     CDS             complement(7787..8791)
                     /gene="trpS"
                     /locus_tag="POB34_000896"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDI5917888.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(8784..9542)
                     /gene="gph"
                     /locus_tag="POB34_000897"
     CDS             complement(8784..9542)
                     /gene="gph"
                     /locus_tag="POB34_000897"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HDI5917889.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(9535..10212)
                     /gene="rpe"
                     /locus_tag="POB34_000898"
     CDS             complement(9535..10212)
                     /gene="rpe"
                     /locus_tag="POB34_000898"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDI5917890.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(10230..11066)
                     /gene="dam"
                     /locus_tag="POB34_000899"
     CDS             complement(10230..11066)
                     /gene="dam"
                     /locus_tag="POB34_000899"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDI5917891.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(11173..12459)
                     /gene="damX"
                     /locus_tag="POB34_000900"
     CDS             complement(11173..12459)
                     /gene="damX"
                     /locus_tag="POB34_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HDI5917892.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(12551..13639)
                     /gene="aroB"
                     /locus_tag="POB34_000901"
     CDS             complement(12551..13639)
                     /gene="aroB"
                     /locus_tag="POB34_000901"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDI5917893.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEIIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(13696..14217)
                     /gene="aroK"
                     /locus_tag="POB34_000902"
     CDS             complement(13696..14217)
                     /gene="aroK"
                     /locus_tag="POB34_000902"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HDI5917894.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(14618..15856)
                     /gene="hofQ"
                     /locus_tag="POB34_000903"
     CDS             complement(14618..15856)
                     /gene="hofQ"
                     /locus_tag="POB34_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HDI5917895.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(15768..16172)
                     /gene="hofP"
                     /locus_tag="POB34_000904"
     CDS             complement(15768..16172)
                     /gene="hofP"
                     /locus_tag="POB34_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HDI5917896.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(16162..16602)
                     /gene="hofO"
                     /locus_tag="POB34_000905"
     CDS             complement(16162..16602)
                     /gene="hofO"
                     /locus_tag="POB34_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HDI5917897.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(16586..17125)
                     /gene="hofN"
                     /locus_tag="POB34_000906"
     CDS             complement(16586..17125)
                     /gene="hofN"
                     /locus_tag="POB34_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HDI5917898.1"
                     /translation="MNLPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLAHSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(17125..17904)
                     /gene="hofM"
                     /locus_tag="POB34_000907"
     CDS             complement(17125..17904)
                     /gene="hofM"
                     /locus_tag="POB34_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HDI5917899.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQQFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGGFAIALGLALGKAY"
     gene            18024..20576
                     /gene="mrcA"
                     /locus_tag="POB34_000908"
     CDS             18024..20576
                     /gene="mrcA"
                     /locus_tag="POB34_000908"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HDI5917900.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(20741..21301)
                     /gene="nudE"
                     /locus_tag="POB34_000909"
     CDS             complement(20741..21301)
                     /gene="nudE"
                     /locus_tag="POB34_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HDI5917901.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            21621..23756
                     /gene="igaA"
                     /locus_tag="POB34_000910"
     CDS             21621..23756
                     /gene="igaA"
                     /locus_tag="POB34_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HDI5917902.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTNIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            23821..24489
                     /gene="yrfG"
                     /locus_tag="POB34_000911"
     CDS             23821..24489
                     /gene="yrfG"
                     /locus_tag="POB34_000911"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HDI5917903.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            24500..24901
                     /gene="hslR"
                     /locus_tag="POB34_000912"
     CDS             24500..24901
                     /gene="hslR"
                     /locus_tag="POB34_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HDI5917904.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            24926..25804
                     /gene="hslO"
                     /locus_tag="POB34_000913"
     CDS             24926..25804
                     /gene="hslO"
                     /locus_tag="POB34_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDI5917905.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(25939..27663)
                     /locus_tag="POB34_000914"
     CDS             complement(25939..27663)
                     /locus_tag="POB34_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HDI5917906.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            28042..29664
                     /gene="pckA"
                     /locus_tag="POB34_000915"
     CDS             28042..29664
                     /gene="pckA"
                     /locus_tag="POB34_000915"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDI5917907.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETLTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(29740..31092)
                     /gene="envZ"
                     /locus_tag="POB34_000916"
     CDS             complement(29740..31092)
                     /gene="envZ"
                     /locus_tag="POB34_000916"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HDI5917908.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(31089..31808)
                     /gene="ompR"
                     /locus_tag="POB34_000917"
     CDS             complement(31089..31808)
                     /gene="ompR"
                     /locus_tag="POB34_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HDI5917909.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            32036..32512
                     /gene="greB"
                     /locus_tag="POB34_000918"
     CDS             32036..32512
                     /gene="greB"
                     /locus_tag="POB34_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HDI5917910.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            32609..34930
                     /locus_tag="POB34_000919"
     CDS             32609..34930
                     /locus_tag="POB34_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDI5917911.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            35368..35595
                     /gene="feoA"
                     /locus_tag="POB34_000920"
     CDS             35368..35595
                     /gene="feoA"
                     /locus_tag="POB34_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HDI5917912.1"
                     /translation="MQYTPDTAWKITGFSHEISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            35612..37933
                     /gene="feoB"
                     /locus_tag="POB34_000921"
     CDS             35612..37933
                     /gene="feoB"
                     /locus_tag="POB34_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HDI5917913.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            37933..38169
                     /gene="feoC"
                     /locus_tag="POB34_000922"
     CDS             37933..38169
                     /gene="feoC"
                     /locus_tag="POB34_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HDI5917914.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            38372..39250
                     /gene="rpnA"
                     /locus_tag="POB34_000923"
     CDS             38372..39250
                     /gene="rpnA"
                     /locus_tag="POB34_000923"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HDI5917915.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(39279..40049)
                     /gene="bioH"
                     /locus_tag="POB34_000924"
     CDS             complement(39279..40049)
                     /gene="bioH"
                     /locus_tag="POB34_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HDI5917916.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            40087..40770
                     /gene="gntX"
                     /locus_tag="POB34_000925"
     CDS             40087..40770
                     /gene="gntX"
                     /locus_tag="POB34_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HDI5917917.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            40829..41404
                     /gene="nfuA"
                     /locus_tag="POB34_000926"
     CDS             40829..41404
                     /gene="nfuA"
                     /locus_tag="POB34_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HDI5917918.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            41765..43081
                     /gene="gntT"
                     /locus_tag="POB34_000927"
     CDS             41765..43081
                     /gene="gntT"
                     /locus_tag="POB34_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDI5917919.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(43126..45210)
                     /gene="malQ"
                     /locus_tag="POB34_000928"
     CDS             complement(43126..45210)
                     /gene="malQ"
                     /locus_tag="POB34_000928"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDI5917920.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(45220..>47204)
                     /gene="malP"
                     /locus_tag="POB34_000929"
     CDS             complement(45220..>47204)
                     /gene="malP"
                     /locus_tag="POB34_000929"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HDI5917921.1"
                     /translation="GLNYQYGLFRQSFVDGKQVEAPDDWHRSNYPWFRHNEALDVQVG
                     IGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPLRLWQATHAHPFDLTKFNDGDF
                     LRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQCACSVADILRRHHLAGRKLHE
                     LADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAWAITSKTFAYTNHTLMPEALER
                     WDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEKVWAKLAVVHDKQVHMANLCVV
                     GGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTNGITPRRWIKQCNPALAALLDK
                     SLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANKVRLAEFVKVRTGIEINPQAIF
                     DIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVPRVFLFGAKAAPGYYLAKNIIF
                     AINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIPAADISEQISTAGKEASGTGNM
                     KLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVEQVKAILAKGYDPVKWRKKDKV
                     LDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYLVMADFAAYVEAQKQVDVLYRD
                     QEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
BASE COUNT        11197 a        13030 c        11894 g        11083 t
ORIGIN      
        1 tggcgcgttg aattttacat cccgtacgtt cctctcaccc taaccctctc cccaaagggg
       61 cgaggggacc gttcagtacg gaatttagct tgacggtgct atccagcatt aaatactggc
      121 aatactctca cgcaaaatcc gcagcaccgt ctcttcaccg ctgcgattcg ggttaatgcg
      181 aatcgcacaa tgttctgatt gtggattcgc ctggcgaaac gttccggaaa ggcgataaaa
      241 gagcggcggg atttcatatt tcgactctgc acccaccggg taaggcaagg caccgcgctt
      301 ttgcgcctct tccagcactc tggcggcaat cggctgatga aactcgacaa tcaacacctt
      361 cgactgcgca ttagcaatca ccgcgctttt cacttccggc accgcaccac cgttaagcaa
      421 agccagcaac cgttcagata cccctgcctg caccgcgtgc atcactggcg caaacaccag
      481 accacgcaag acttccagcg cctgtgcgcc ctggatctgg ctaccgccgg agtaaagcgt
      541 ggcgcgaata cggttgataa catcagcatc gccgaccact gcaccaacac cctctggccc
      601 aaatagcttg aagcaggaaa atgtcgagac attcgcgccg cattcacagc cgattcgcgc
      661 caccttcatc accgcatagt tgtcatcggt taacgctgga acacctgccg cgcgcaacgt
      721 tgccagcaca tctgccagca cgtagctgtc ctgcggctgc tggcgcgtat gctgcaccag
      781 cgccgcatcc ggttgttgct cgtcgacgac ctgcttcagt gccgacaggt cattgaaatc
      841 aacagtaata agcgtcagcc ccatctgctc aataataacc cgtgtcgtcg ggtaaacagg
      901 cgcgtcatgc accagaagac gctgccccgg tttgagcaaa gccgccagcc cggcgcgaat
      961 cgcgccagtc cccgcgccct gcaccagcgc cgcagcctgt gcgtgaaatg catcagccag
     1021 cacctgttcc acacgctggg taacgcgcgg ttgattcagt cctggcgtta agcctaaatc
     1081 accgccagta agaaactcgc tgccggggaa atggcgacaa atgctatcca ccagcgcaaa
     1141 ctgcttttgc tgcgcctcaa taatcgtcag gctttgcaga ggaaacgtct tcataggtgc
     1201 cttccttaag ccggaacgaa caggccaagc cagtaaagaa cattcagcag aataccggtg
     1261 atcattactg ccacgaccgg tgccgccatt ttctgtaccg gacggcccag ggattcgttg
     1321 aggaagtaaa tggcaaccgc gatagagaat ccggtataac ccgccatctt aattgctgcg
     1381 aaaatcgaac cgaccagcag cgccacttcc atcagcatat tcatggcgtt acggatgtta
     1441 tccgacgcat tacgcaccga cgggtagcgt cccagccatt tgccgatcga acgaagcagc
     1501 aagacttccg ccgaaataac cactgcgcct aataccgctg caaccatcgg attcggcgag
     1561 agatagccca ccgcataaac aaaggtaaag cccgcaactg catacacacc cgttgctaag
     1621 gcggtggtgg caatcaacgg tacaaaaccc agcccgcgca taaattctgc cagtgccgcc
     1681 tggttgatca gcgtttgcga ttgttccggc gttacgcctg cggagtacgc tttctccaga
     1741 gtgaagatcg acacttcact gccggcgaaa atcttcatgc tggcaacggc ggcaatcaat
     1801 gctcccacga tggcgatata gggtaagttt ttgataatcc gcgacgtgcg ttcttcaaac
     1861 accgaaagcc cgctggcatc aatatcattt tcatcacgat gacgcaggtc gtgagttatc
     1921 gcgatcccca gcagcatcac catgccaata aagatttcga tggattcagg gttaagatgt
     1981 gggaaatagc gcacgactac cacacgggtc atcagaacaa ccacggcggc gaccagactt
     2041 tgcttccagc caaactggta gaaaatcgcc accagcggga acagtgcaaa agcagaaacc
     2101 accggcgagc ttaattcccc gaggctacct aatacatcca ccggcagcgc ggtcagcagc
     2161 tggtttactg gcaacaggca agtaaggatc aatacgcccc agatagcgcc aagaccaaac
     2221 gccatcaggc tgtttatcgc cagtacgccg aggatatcgg taggaagaaa gagtaaccat
     2281 gcgttgagca gtccggtttt cagggtaaaa gagatcccca ccgaggccac aaaaccaatg
     2341 ctcagaccaa aagcgatact ccccgcctcg cgacggttca tatagccttc aatcagttgc
     2401 ggcaggatcg ggcggatgcc gtcatgaaaa acagccgccg agcgatgcgc cagaagcgat
     2461 gtcatacccg tcaggcacgc caccacgata atctgaatat acagatccat agtcgtgcct
     2521 tttatttcag atggttaatc agcatcggaa tcgcgtgttc gacgtgctca acggaaaggc
     2581 caaacgccac ttttccttca gcgatcattt tggcgatatg ctcgtctttc gctttaatgc
     2641 ctggtttggc aatggtgcaa cttttgttat agccaatcac cgcgatggca atcgacaatg
     2701 ccgcgcccgc accggtatta cacgcaccga tgtagtaatc cagttgcccg gatttcactt
     2761 tcattgccgc ttccatgtcg ttgtgaatga aaacttcaaa actgccagga gccgtcgctt
     2821 caatggtttt tttaatctgc tcacgctgta agcctgcaac gccaatcttt ttcatgagtc
     2881 tgtccttatt ggaaaaattg agagggagtt tcacgtaaca tcacatccac atcggcctga
     2941 ctgaatcctg actggcgcaa ttgcggaata aaggtggtca ataaatagtc atagccataa
     3001 ccaccattgg cttttaaatg ggagcggcgc gtaatatcca tcgacagcat gacgcggttc
     3061 agcaacccac ggtcacgtag cgcgtggagc atcgcaatgc gcttttcgtc cgggtagtaa
     3121 ctgttcttac caatggtgtc gaactgcacg tacgcgccga gatcgatcat cttcaaaatg
     3181 ttgtcgaggt tgtctttcag atcgcagtga ccaacggtga cgcgcgaaag atcaactccg
     3241 tgggcttgca acagagccag ttgttccagc cccatggtgc tgaacgaagt atgcgtggag
     3301 atcgggcgtc cggtctggtt atgcgccagc gcagcggcaa taaacacctt ctcttccagc
     3361 ggcgtaatct tcccttcgct agagccgatc tccgcgataa tcccggcttt caggtcggtg
     3421 ccatcgatac cctgttcaat ttcatcgacc atctcctgcg ccagttcctg cacgctgcgg
     3481 gtcgccacat gctccgggaa aaaagcgtcc tggtaataac cggtgcaggc caccacgttg
     3541 atccccgtct cgcgcattac atcgagcata aattgcgcat tgcgccccat gtaacggttg
     3601 gtcatctcaa tcacattacg cacgccccgg gtcatcaggt cgttcatctc ctggcaaatg
     3661 aacgcatact gatcaaggcg gcagtccacg ttgtttttaa agccggagag atcaatatgc
     3721 agatgctcat ggaccagggt gtaacccgtc ggatcaaaac tcatagggtg tctcctgcaa
     3781 accggaggga ggaaagaaaa gagcgaccat tttgtggcgg tggcgcacgg aaaaattcac
     3841 acacggtagc ccccacatct gaaagcgtgg tgcgcacacc gagctgcgta gctatcatcc
     3901 cttgctgata aaccagcact ggcaccactt cgcgggtatg gtggctgtga ccaatggtcg
     3961 gatcgttacc gtgatccgcc atcacgacca ggcaatcatc tggctgcatc gcctcaacaa
     4021 gccgggcaag gttacggtca acgacctgca aacgttcggc ataacgtgcg acgtcttctg
     4081 catgaccagc gaggtcggtt tcctggatat tggtgcaaat aaacgccgtc ggatgcgtgt
     4141 taaattcgtc aagggtgata tccataatcc gctggctatc caccaggttt tgccagctca
     4201 cgccataagg attgctgacg atatctgcca ctttacctac cagcacggtt ggcacgcccg
     4261 cctcatacag tttttgcggc acctgcactt tttcatcaac accgtagccc atatgcacga
     4321 cctggaaacc gttgtcataa gcgccagaac gcggcgcatt gatgccaata aagcgccctt
     4381 ctttgctttc tgcggcatcg agaatacgct gactgtcggt taacaagccg ccaaatgtaa
     4441 tgacccgacc gacctgtacc tgctcacgca cgatacgacc aattttgatt gcgtcgtcaa
     4501 aagagatcac agagagattg gcggtaatgt tatagacctg gcctaaatcc gcctcgagat
     4561 tatcgccaat cgcaaccgcc tgattgaccc acagaaattg cagttcatcg ccacggcgct
     4621 ccacctgcca accggcggat actaatgcct gctcaacacg gtcaatcaca tcgcaaaaag
     4681 gcatccgcag cggcggtaac gggcgcgtgc ctaaaatttc ctgatgcccc ataaaggtat
     4741 cgccaccttc atgttgcagc tctgccacgc cccaggttgc ggaatctgac ggctgcatat
     4801 cgcctggcgc ataacccaat gcgttgatta gccccagctt ctccagcgtt ggtagctgca
     4861 aatgcggcaa ctggctcagg atgtgaccac aggtattcgc cccggcatct tgcggacgca
     4921 ccagcgtgac atctttcatt gcccctacgc caaagctatc aatcactaac accacaaatc
     4981 gcgccattac gcccccagag agtttccaag actgtcataa cgaccaacga ttttcggttc
     5041 gccacggtga ataccggaca ccagcaccac atcgctacgg gtgacaaaaa tctgcgtgcg
     5101 gaaacagagc accactgcac tgctcaccgg atactcgcct gccagcggca gggtgtagtc
     5161 gatactgctg tcatccacag ttttgagatt ggtttcagta atcttttgat tttctggcgt
     5221 aaacaccagc gcatgttgcg catgaccacg acggtagtaa ccgccgccgt agcagtagct
     5281 gtcgccacgg aaatgatggg agatttcact taaccagagc atcgcgatac gttcaggctg
     5341 atcgccctgc tggtttgccg gaatagtgcc cgtcagcgca tgaccgggtt cggcatgagt
     5401 cacaccgtat tccgccagca atggcagcga agtgcagctg gtcgctgaag gcgcgttcag
     5461 ttgctcaagt gcaataccag atttcgccag ttgatcccgt gcctgtatca gcgtgtgaag
     5521 attcggtgtc ggcaaaactt ttccggcagc ctcatcccaa agcaggcaag ggaaatgggt
     5581 aagtccggct aaatgcagcc ccggcagttt cttaatttca gcaaccacct cgtgcagcga
     5641 atgctgtaca aaaccgctct cctgccccgg ataaagaaaa tcatcgtcgc tataaacttt
     5701 gagcagcaca aactgcacac gtccggcctt caccgctgcc gcagaaactt cccgcgcttt
     5761 gtcgagagta aacacggtga tgacgtcggt gccctgttca acggcgtcag caacctgatg
     5821 acaagggatt tgtaccagat gcccctgatg cgccaccgac aaaccagcgc ggcgcatgac
     5881 tcgcgcctct ttgtaatcca ccgccacaat gccgctgtaa cctaatgcca gcaatttttc
     5941 cgccagccat gggttacggc caaactgctt ggtcatcaga tacagttcaa tgccgtaaag
     6001 ccgcgccgtc tcaatcagtc gcttaccgtt ttccagtacc tgatccacgt cgatcaccca
     6061 gctgtccggg gcgatcttgc cctgctgcca caaacttagt gcggcggaaa tcagcgccgg
     6121 gttctggcgt ttcaatgctt ctacaaacat cttgttgcct cgtttctgtt ccttgaatta
     6181 aatattcatt tttttgaata tttaggttgt gcgacgtgga tttgtcggga gcttcaaacc
     6241 tcttcgttag cagccatcca taatccataa aggttggcca gcaaatagcc ttcttcacac
     6301 gggttaactt ccagtgcgaa ttccttcaac aatacctgat gcaattgcac aacagcttgc
     6361 cagtggctgg attgcgccag ctctgccagc aattcgttat ccagcggctc tatttcttca
     6421 ccacggcgac tgcgcatcag tgcactcgcc atatgtgtaa tcgccatcgt tccttgctca
     6481 ctgcgcactg gcagatggca ctctgtttcc agtacgttga cgacctgcat catgcctttg
     6541 cagatgtcct tatcaataac gcctgcctcg caaagcaggt tgagtctggt ttccatatca
     6601 gatgcctttc gtttaataac tcggttcctg ttccccactc accacgcgtt gttgatgggc
     6661 gagcagggcg tgtttatcta caacggcacg cagcagttgt tgcattgggt aatcatcggc
     6721 tcgcgtcagg accacggctt cggtggcctg taaaaatcgc ggatcgtctg tgagcggcgt
     6781 cgcctctaat cccagcatgg tcagttcgtt ttccgccacc acgttccaga tcaccgcatc
     6841 gacatcgcct ttaacaatgc gttgtaaact ctcgtgataa gagagatcga ctcgttccac
     6901 atcactaccg ccaaaaaaaa catcggtcat gattttctga tccgccgaac ggttatccag
     6961 ccccacgcgc ttcacgtttg cggactcgcc tttacggcaa atcaactggt gctcgccaac
     7021 gtaggtgtgc ggccccaact ccagcgcgag gcataaccct ttttgcgtga gataactttc
     7081 cgccgccagt cgggaaacca ccgccatgtc atacacgccg ttaagtaaac actccacgcg
     7141 aatatccgcg ccgcgcatat gcgcatagta aaaaggaatg ccatcaaact gagctttcag
     7201 cccgctcgct aaaccttcgt acaagcgggt atagggcaaa ggcatcgcac ataccacgtt
     7261 gttgatatcc acatgactca gcaatgcttt gttatccatc tcgaccagat aactgccatt
     7321 gcgcccacgg cgttcaatcc gtatcgctcc gcttgattcc agcgttttca acgcggcctg
     7381 cgtcaggcca accgatgaac ggcattcagt tgccagctca tctatggttt tcaacctatt
     7441 accgcatttt tcacccaaca aataacgggc cagcgtcgtg ataacgacgc cttctttttt
     7501 gataaacgtt cgacgcataa tatattttca ataaaatgaa tgtttatatc ttcattaaaa
     7561 tgaagataag tggcaactgt gagatgtgga aacggcgagg cgcttcacat tttttcggat
     7621 tattgcaaat gaggataaaa aaaccgggtt tccccggttt cagagtgatg acgaagtgat
     7681 ttattcgaca atgcgagcaa agggtaattc ccactggcgc ttgcctgctc ccctcaccct
     7741 aaccctctcc ccaaaggggc gaggggaccg tccgagcgtt ttgatattaa ggcttcgcca
     7801 caaaaccaat cgcttcgtac accgctttca gcgtacggga agcgtgtaca ctggcttttt
     7861 ctgcgccgtc tttcatcacc tgttgcagga aggcttcatc gttgcggaaa cggtgatagc
     7921 gttcctgcaa ttcagtcagc ataccggaaa cggcatcagc cacttcacct ttcagatgac
     7981 catacatctt gccttcgaac tgtttttcca gttctgggat gctctggccc gttaccgcag
     8041 aaaggatatc cagcaggttg gaaacgcccg ctttgttctg cacatcgtag cgaactaccg
     8101 gcggctcgtc ggagtcagtg accgcgcgtt tgattttctt cactaccgat ttcggatctt
     8161 ccagcaggcc gataacgtta ttgcggttat cgtcagactt ggacatcttc ttggtcggct
     8221 ccagcagcga cattacgcgc gcgccagatt tcggaataaa cggctccggc accttaaaga
     8281 tctcgccata cagcgcgttg aaacgctggg cgatatcgcg actcagttcc aggtgctgtt
     8341 tctggtcttc acccaccggt accagattag tttgatacag caggatgtcc gcagccatca
     8401 gcaccggata gtcaaacaga ccagcgttga tgttctcggc ataacgcgca gatttatctt
     8461 taaactgcgt catgcggctc agttcgccga agtaggtata gcagttcagt gcccagccta
     8521 actgcgcatg ttctggtacg tgggactgaa caaaaatggt gcttttctca ggatcgatac
     8581 cacaagccag atacaaggcc agcgtatcca acgtcgcttt acgcagcttc tgtgcatcct
     8641 ggcgcacggt gatcgcgtgt tggtcaacga tacagtaaat gcaatggtag tcatcctgca
     8701 tgtttaccca ctgacgcagc gcacccatgt agttaccaat ggtcaattca cctgagggct
     8761 gtgcgccact aaaaacgatg ggcttagtca tttttcgatt cctgattttc gctatgcgga
     8821 agccctaatg cgggcagaag gtcatttata gactgataaa ttacatcagg ctggctgaga
     8881 tcgatagcct cgccgtagtt atatccgtag gttaagccaa ctgatgggca acctgccgct
     8941 tttgccgcct gaatatcatt gcgtgagtcg ccgacaaaca gcatctgttg tggggcaatt
     9001 cccatccgct cagccaccag taacagcggg tccggatgcg gttttttgtt ttgcacatca
     9061 tcgccgccga taaccacgct aaagtatttg gcaatatcta aggcttcgag cagcggcgcg
     9121 acgaacggcg tcggtttgtt ggtgaccagg cctagcggca ggcctttagc ctgcaacgcg
     9181 cccaacgtat cggcaacgtg cgggaacaaa aacgtcccct cttcggcaac ctcgccatag
     9241 tagcgatcga acagtttacg cagaatacgt acctgttctt ctgccggaat gtcgtcatca
     9301 acgggcggtt tacccattgt tttacgctga gtcgcacgtt cctgacgcgc ccaggtcaat
     9361 gcgcgctcca tcagaacatc tgcgccgtta ccaatccagg taataacgcg ttcttcacct
     9421 gcgacgggca actccagcgc atacagcgcc atatctaccg cagcagcaag accgggagca
     9481 ctgtcgacca gcgtaccatc aagatcaaaa gcgacgccgc gaatatcttc aaacttattc
     9541 atgacttacc tttgccagtt cactgcgcat ttcatcaatg acttttttgt agtctggctg
     9601 gtcgaagatt gccgaaccgg cgacgaacat atccgcgccc gccgcagcga tttcgccaat
     9661 gttgttcacc ttcacgccac cgtctacttc cagacgaatg tcaaagccag actcgtcgat
     9721 acgacgacgt acttcgcgca gtttatccag tgtttgagga atgaacgact gaccgccgaa
     9781 accaggattg acggacatca gcaggatcac atccagctta tccatcacgt aatccagata
     9841 gctcagcggt gtcgccgggt taaataccag tcccgcttta cagccatttt ctttaatcag
     9901 ttgcagcgtg cggtcaacat gctcggaggc ttctggatga aaggtaatga tgctggcacc
     9961 agcggcagcg aaatcaggca caatgcgatc gacaggtttc accatcaggt gtacgtcgat
    10021 aggggcggta atgccgtagt tacgcaagga tttcagcacc attggcccaa tcgtcagatt
    10081 gggaacatag tggttatcca tgacgtcaaa atgcacgaca tcagcgccag ctgccagggc
    10141 ttttgcggta tcttcaccca ggcgggcaaa atcagccgac agaattgagg gggcaatcaa
    10201 atactgtttc atccgcttct ccttgagaat tattttttcg cgggtgaaac gactcctggt
    10261 ttgtacaaag ccagcagttc gtccaccttt ttacgtgtgc cgccgttgct gcttatactg
    10321 cgtcgaactt tgacgacatg caattttgcg cgctgatacc actcacgcgt taacatcgta
    10381 tcgtgattgg agatcagcac tggaatatgg cgctcaacca gaccttcggc gatctccgcc
    10441 agatgcgctt gttgttcaag cgtaaaactg tttgtgtgat acgccgtaaa gttggcggtc
    10501 gcagacagcg gtgcataagg cggatcgcaa tagacgacgg atgcatcatc tgcgcgcgcc
    10561 atgctatcgg cgtaagactc acaatagaaa aaggcattct gcgctttttc agcgaagtga
    10621 tacaactctg cttccgggaa atagggtttt ttgtagcggc caaacggcac gttaaactca
    10681 ccgcgcagat tgtaacgaca caggccgttg taaccgtagc ggttcaaata taaaaacagt
    10741 accgcccgac ggaacggatc ctggcttttg ttgaactctt cgcggaactg atagtaaacc
    10801 tcggcgcaat ttgtttcggg aacaaacagc tcgcgtgcgg cctgtacgta ctcatcagta
    10861 cgcatcttca caatgttata gagactgatc aggtcgctat tgatatcggc aaggatataa
    10921 cgagaaaagt cggtgttgag aaacaccgac ccggcaccta caaaaggctc aaccagacat
    10981 tcgcccttgg gcaaatgccg tttaatatca tcaagcaggg gatacttgcc ccctgcccac
    11041 ttcaaaaaag cgcgattttt cttcatgctg actaactaat tacaccttct ccggctgtgg
    11101 agaaagctcc gacagcatcc agcgcttcag gcaactcccg caacgataaa ccattgcggg
    11161 agataacctt gattacttca gatcggcctg tacctgacgc agcggtttcg cccacgggtt
    11221 tttggcctga acatctgctg gcaatgtaga taccgctttt ttcgcctctt ctttcgaagc
    11281 gtacacgcca gaaaccagga cataccacgg ctgaccatta cgcgtcgttt catagacaac
    11341 gtagtttttc agattctctt tcttcgccca accgttcagg ttgtcgtagt tagaggaact
    11401 gctcagctgc agagtgtaat ggctggacgg tgccgatttc aacgaaccaa cattacctgc
    11461 ggtcttccct ccagcggctg gtgttgccgt ggtttgcgcc ggggatgccg tctgtactgg
    11521 agccgtagtc gccgtctctt ttggtgctgg cgtagaagtc gcagccggtg ccttcgtgct
    11581 cgccacaggg gcagctggtt cagtacgctt cggcgtctgt gctaccggct tcggctccgt
    11641 tttcacggtt gcttgcggtt ttttcggttc aatcaccgcc tgctgacgag ctgggcgcgt
    11701 agtggacgga cgttcagcgg tttgcgtttt cgccgtgtca cgcgatgcat tgccattgcg
    11761 aacaggcgcg acagtcgcgg gttcggtcgg caatgtggaa ttgaccgcca cattgttcaa
    11821 ctgttgctga ttttgtggct gggtcagcgc attgttcagg tcaccctgca cttcaacacg
    11881 ttgttgacca tccgttgccg ccggggtttg cccttgagtc ggcgtagaag agatcggtgg
    11941 cagagaaaca tcctgctgag tattttccgc agacgtggtt cccggcgctg gctgcacgcc
    12001 attcgcctga tcggtcgcat tgcctgcaag atcaatactc ttctcgccag acgcggtttg
    12061 atcgctggaa gaggtcgagg gggcttttag cgcagaaccg ataccgatga tcaacagcag
    12121 tagaaccaga atgccgacgc ccatcatcat atactgacga gaagcgggtt tactggctgc
    12181 ttttttgcgc ttacgcggac gacgctctac gcgctcttca tccacggttt catcttcgga
    12241 ttcgtcaatt tcttcttcga tttccggttc ctcattgcgc tctttttgcg cacgagtcgg
    12301 acggcgatcg tcagtgtcat caagttcaat atcatcaaaa ttgatctgcg gttcgccacg
    12361 ttcagtacgc tcagaacgtt cagaagattg acgagaacga ccagtacgac gatcgctggg
    12421 atcgggtttc agctcgtctt ctggtttgaa ttcatccatt taacacccca ctaaaaggtt
    12481 aatgcttacc acgttgcaat taacctgaag ctaatagacc gcttgataag cggcctgacc
    12541 tttcttgttg ttacgctgat tgacaatcgg caatggcgtt aagaacaagc tcgtgcgaaa
    12601 cgccgctgcg aacttcactc ttaccaattg ccaacggaag aattaagcgt atctctcccg
    12661 caaggacttt cttgtcacgc agcatatgcg gtaaatacgc ctgcgcggac atttcgcgcg
    12721 gcccattgac cggtaaccca gcccgcttga gcagggttat aatacgctgc gtttcggcag
    12781 aactaaactg cccgagacgt tccgacgtcc gcgccgccat caccataccc gcagcgaccg
    12841 cttcaccatg taaccaattg ccatacccca tttcagcttc aatggcatga ccaaaggtgt
    12901 gtcccagatt cagtaaagca cgtaacccgg tttcgcgctc gtcggcggcg acaacttctg
    12961 ccttcagttc acaacaacgg cgaatacagt acgccattgc cggaccgtcc agacgcaaca
    13021 acgcatccag attctcttcc agccagttaa aaaacgcacc gtcaagaata atgccgtatt
    13081 tgatgatttc tgccagcccc gacgctaact cacgcggggg aagcgttttc agacagtcga
    13141 gatccaccac cactgaagca ggctggtaga acgcgccaat catgttttta ccgaggggat
    13201 ggttgaccgc agttttgccg ccaacggagg aatcgacctg cgacagtaac gtcgtcggga
    13261 cttgaatgaa acgaacaccg cgctgataac tcgccgccgc gaagccggtc agatcgccca
    13321 ctacgccgcc gccaagcgcc accagcgtag tatcgcgacc atgcggcttt tgtaacaacg
    13381 ccgtaaagac ggtatcgagt acagccaggc ttttatactg ctcgccgtca gggaggataa
    13441 cgctatcgac gttaacaccc gcctgttcaa gtacgccgcg gaccttatcg agatacagag
    13501 gagccagggt ttcgttggtg accaacatga cctgctcgcc cgatttcagc ggtaagaatg
    13561 aagctggttc attaaacaaa ccagatgcga tggtaattgg gtaactacgt tccccgagag
    13621 taacgacaat cctctccata acgcgacatc caccttaatt actgtacccg cagacgagtg
    13681 tatataaagc cagaattagt tgctttccag catgtgaata atctggtttg caaccacttt
    13741 agcgctttga tcatcagtac gaatggtcac gtcggcaatc tcttcataca gcggattgcg
    13801 ttcattggcc aacgcttcca gaacttcacg cggcggtgtt tcaacgtgca gcaacgggcg
    13861 ttttttatca cgctgcgtgc gtgcaagttg cttttcgatg gtcgtttcaa gataaacgac
    13921 aacgccacga gcggaaagac ggttacgcgt ttcacgggat ttcacagagc cgccgccagt
    13981 agccagcaca ataccctgtt tctcggtcaa ctcattgatg accttttctt cgcgatcgcg
    14041 gaagccttct tcgccttcta aatcgaaaac ccagcccaca tcagctccgg ttcgtttctc
    14101 aatctcttga tcggaatcgt aaaattccat attgagttgt tgagctaact ggcgcccaat
    14161 agtgcttttt ccggcaccca taggcccaac cagaaagata ttgcgtttct ctgccatttt
    14221 ttcggtacta ctaagactat tcgttaatga taaacccgct tcgctcagag agcgccgcag
    14281 gacatgaact gaaacctcat aagatattgc gagagtcaga ctgaaaatta tctcaatact
    14341 ccagcgggtt tggcaactga ataaatcacc catccctctg catatctggt cgctgcaagc
    14401 gcgctgcctt gctaccaccg ctctggcgat aaatcaccgg gtaagattag cgtaaaaaag
    14461 acagcaaaat gccgcctgaa tgataaatca tcatcatgga acgcaggcag cgaagagcac
    14521 caactctcaa atcggtactc cttgtatgct aaatacctgc gcgcgtcaaa tagatgaaac
    14581 acgttcagca taaaaacatt accgctttta cggctgttta ctcactggaa accagtcgtg
    14641 gcgtgataaa caccactaac tcgcgtcgtt catcttcttt tccgtcatga cgaaataatt
    14701 gcccgaacca gggaatgtcg ccaagcaacg gtacgctatc ctgacccgat ttatttttac
    14761 gggtaaaaat gccgcccagc gccaacgttt ctccgctttt gacctcgacc tgcgtttcga
    14821 tctcctgctt atcaatcgcc agcacttcgc catcggcctg ctgtagcacc tgccccggaa
    14881 cgttctggct gatgtgtaat ttcagccgga tgcgaccttt ttgtaacacc gtgggcgtga
    14941 cctccatccc caggacggcc tctttaaatt ccaccgacgt cgcgccactt tccccgctgg
    15001 aaacctgata tggaatttcg ctcccctgtt taatgctggc aggctgaaga tgtgaggcca
    15061 gcagacgcgg actggcgata atatccagct gctgtttttg ttcgagcgca gaaagctcaa
    15121 gatccagcaa gcgtccgttg atgcgcccaa tgttaaaacc gacatgcgtt gtcgccgtcg
    15181 ctacggagag gtcgctacca agcgtggtga cttgcccaac gccaccagcg tgttgcgcat
    15241 cggccagcgt ccatttcacg cctaactcac gcaaactttt ttcattaatg gtgacaatat
    15301 gcgccgacag ctcaacctgc ccgaccggca gatccatttg cgctacccac tgttcaagcg
    15361 cgcttaacgc cgttttgtta tctcgtagca aaaggcgatt ggtgcgttta tcgacggtca
    15421 tactcccttt ggcactcagt agcttctccc ccgctttcgc cagttctccc gcgtcggcgt
    15481 attgcagggt tatactgcga ttttccagcg gcagatttgc ctgcgcccgc gcctgctccg
    15541 cctcctggcg ggcgatattg ttattctgcc aggcaatgga atgcactgag agaatgttgt
    15601 cttcctgccg cgttatcagt ccggcgcttt tcactacagt ttgtagtgcc tgcttccagg
    15661 gaacatctgt tagatgtaac gacaccgtac cgctgacgtc tggcgacacg accaggttca
    15721 acttctcctg ttcagccagc gcctgcaaca cctgagctac cggaacgtca tccaccatca
    15781 gcgtcacttt ttgcggcttt gccgcctgta cgccgggtat cagcatcaac agtagtgcgg
    15841 ctatccattg cttcatttgt atctccttgc cgttgccaca accattgtgg cggttcgcag
    15901 tttgtcccgg tacccagcgt tagtacgtct ggcgtcagct gtaaaattgt ccagccgttt
    15961 tccagcacat cgttttgctg cacccgtcgc catttctttt gcccgtcttt tattacaccg
    16021 atgatgcgct cgcctcgccc taccatcccc tgatagcgcc actggctaag ttcgctaatc
    16081 cggcatagat cttccggcgg tttaaaaggg tcacgcatac cggttaaaag gcacaatgca
    16141 atacctgcca acaaccagcg tttaaccctc atgcggcgtc tccagttgta gcgtgaacaa
    16201 aagatcatca ccttccacgc ttaacgaaaa acggctcacg ctgacgttgc gctctgccag
    16261 ccgtgtaaat gccgacggca ctgcttccca cagcgttttc aacgccaact cgcctccctg
    16321 cgcggatgga tgccaggaaa ccagttgcgc gccggataac tgaaaatcca gtggcgaaaa
    16381 gggcagcgtt ttttcctcgc tgaagggagt ggtgtctacc aggcgataca gtgcggccca
    16441 ctgctggtga tgacttgccc gtagtcgaat taatgcgtcg cgctcttcat ggtgtgtcga
    16501 tgacagaaaa atgagcgtaa ctaacatcag caaccagaat gcccagcaaa actggcggag
    16561 gcggggtgat gtggcgaacc accagtcaaa gaacatgttc atcgctaacc ttccttgtta
    16621 actgatactc aaattgccag cgtccctgcg catcctgctg cgtggccccc cgctgattga
    16681 gatgaaaaga agcatcctgg cgaagtgacg tttctagtgc gtttaacgcg gtaatgcttg
    16741 ttgtaagccc cttgatctcc agcgttccct gctgccagct tatcgttgtc agccaggcgt
    16801 gttcaggtaa aagagccgcc agtgcttcca gcgcagattg ccagtcgcgg gtaaattgtc
    16861 gctggcgctg cctttgcaaa cgctgctcac gaatttgttg gcgctccagc aaacgtggtt
    16921 tcgttatctg taagctgtgg gcgagttgtt gttccccctg aagcaaaacg gcgtctacgc
    16981 gagcttcggc gctgcttgtc agacgcagta ttagcgttat cccgacggcc agcagcagag
    17041 gcgcaacgaa catcagcaac cagaaacgca gaaaagcggt ccggcgttgc tgtcgccagg
    17101 gcaaaaaatt aattggcagg ttcatcagta cgccttccca agcgccagcc ccagcgcgat
    17161 ggcaaagcct ccacctggcg gcggtagcgg cggctgacga acagaaacgg cctcccaggg
    17221 atcaaatccg ccttcgccac atatcgcgac gctgtcggga tcaacggata acgctgccgc
    17281 cagctccttc gcgctagtca tccctaccgc cagtttgcgc ccccagcgat agcgtgtcgc
    17341 ccacagccac tgttcgttat cacgccaggc cagacattgc tgatgagaag gtaaaaaagg
    17401 caggaattgc tgtaatgcac tggcatccgg ggtgatcgca ctaacatgaa cacgcaacct
    17461 ttctgccagc gtaagcagcg ttgccagctc tttgctttgc gcggcagtca cgttataagc
    17521 ggggctgagt gagtcttcgc tataatcgaa gcgcagggag tccggatcca tatccagctc
    17581 gcgggccatc gtccctgaca gccaggccgt ttgctcccgc tcaccaaggg acatcgacgg
    17641 gcgcggaaat gaccgctgta atgtgcgact ggcgggaaac gccaacataa tgtgatgacg
    17701 ctgcggcagt tcgcgactcc aaggtaacaa cgttttagcc agctgctgcg catcaacaat
    17761 ccgcccatct ttgataatgt cgttctccag cggcaaccgc caccagcgtt gcaaaaagca
    17821 ttcttttgcg ccccgtacga tcgcaaccgc taccgcttct tgctgttgta aatgcaaacc
    17881 aatttgccag atcttaaatg ccattgtgat gatctcctta tcacccgtca ctctgacggg
    17941 tatatcaatg cgtctggctt gcctttatac taccgcgcgt ttgtttataa actgcccaaa
    18001 tgaaactaaa tgggaaattt ccagtgaagt tcgtaaagta ttttttgatc cttgcagtct
    18061 gttgcattct gctgggagca ggctcgattt atggcctata ccgctacatc gagccacaac
    18121 tgccggatgt ggcgacatta aaagatgttc gcctgcaaat tccgatgcag atttacagcg
    18181 ccgatggcga gctgattgct caatacggtg agaaacgtcg tattccggtt acgttggatc
    18241 aaatcccacc ggagatggtg aaagccttta tcgcgacgga agacagccgc ttctacgagc
    18301 atcacggcgt tgacccggtg gggatcttcc gtgcggcaag tgtggcgctg ttctccggcc
    18361 acgcgtcaca aggggcgagt accattaccc agcagctggc gagaaatttc ttcctcagtc
    18421 cagaacgcac gctgatgcgt aagattaagg aagtcttcct cgcgattcgc attgaacagt
    18481 tgctgacgaa agacgagatc ctcgagcttt atctgaacaa gatttacctc ggttaccgcg
    18541 cctatggtgt cggtgctgcg gcacaagtct atttcggtaa aacggtcgac caactgacgc
    18601 tgaacgaaat ggctgtgatc gccgggctgc cgaaagcgcc ttccaccttt aatccgctct
    18661 actcaatgga tcgtgccgtc gcgcggcgta acgtcgtgct gtcgcggatg ctggatgaag
    18721 ggtatatcac ccaacaacag ttcgatcaga cacgcactga ggcgattaac gctaactatc
    18781 acgcgccgga gattgctttt tctgcgccgt acctgagcga aatggtgcgc caggagatgt
    18841 ataaccgtta tggcgaaagt gcctatgaag acggttatcg catttacacc accatcaccc
    18901 gcaaagtgca gcaggccgca cagcaggcgg tacgtaataa cgtgctggac tacgacatgc
    18961 gccacggcta tcgcggcccg gcaaatgtgc tgtggaaagt gggcgagtcg gcgtgggata
    19021 acaacaagat taccgatacg ctgaaggcgc tgccgaccta tggtccgctg ctgcctgccg
    19081 cagtcaccag cgccaatcct caggaagcga cggcaatgct ggcggacggg tcgactgtcg
    19141 cattgagtat ggacggcgtt cgctgggcgc gtccttaccg ttcggatact cagcagggac
    19201 cgacgccgcg taaagtgacc gatgttctgc aaacgggtca gcaaatctgg gttcgtcagg
    19261 ttggcgatgc atggtggctg gcacaagtgc cggaagtgaa ctcggcgctg gtgtcgatca
    19321 atccgcaaaa cggtgccgtt atggcgctgg tcggtggctt tgatttcaat cagagcaagt
    19381 ttaaccgcgc cacccaggca ctgcgtcagg tgggttccaa catcaaaccg ttcctctaca
    19441 ccgcggcgat ggataaaggt ctgacgctgg caagtatgtt gaacgatgtg ccaatttctc
    19501 gctgggatgc aggtgccggt tctgactggc agccgaagaa ctcaccaccg cagtatgctg
    19561 gtccaattcg cttacgtcag gggctgggtc agtcgaaaaa cgtggtgatg gtacgcgcaa
    19621 tgcgggcgat gggcgtcgac tacgctgcag aatatctgca acgcttcggc ttcccggcgc
    19681 aaaacattgt ccacaccgaa tcgctggcgc tgggttcagc gtccttcacc ccaatgcagg
    19741 tggcgcgcgg ctacgcggtc atggcgaacg gtggcttcct ggtggacccg tggtttatca
    19801 gcaaaattga aaacgatcag ggcggcgtga ttttcgaagc gaaaccgaaa gtagcctgcc
    19861 cggaatgcga tattccggtg atttacggtg atacgcagaa atcgaacgtg ctggaaaata
    19921 acgatgttga agatgtcgct atctcccgcg agcagcagaa tgtttctgta ccaatgccgc
    19981 agctggagca ggcaaatcag gcgttagtgg cgaagactgg cgcgcaggag tacgcaccgc
    20041 acgtcatcaa cactccgctg gcattcctga ttaagagtgc tttgaacacc aatatctttg
    20101 gtgaaccagg ctggcagggt accggctggc gtgcaggtcg tgatttgcag cgtcgcgata
    20161 tcggcgggaa aaccggtacc actaacagtt cgaaagatgc gtggttctcg ggttacggtc
    20221 cgggcgttgt gacctcggtc tggattggct ttgatgatca ccgtcgtaat ctcggtcata
    20281 caacggcttc cggagcgatt aaagatcaga tctcaggtta cgaaggcggt gccaagagtg
    20341 cccagcctgc atgggacgct tatatgaaag ccgttcttga aggtgtgccg gagcagccgc
    20401 tgacgccgcc tccgggtatt gtgacggtga atatcgatcg cagcaccggg cagttagcta
    20461 atggtggcaa cagccgcgaa gagtatttca tcgaaggtac gcagccgaca caacaggcgg
    20521 tgcacgaggt gggaacgacc attatcgata atggtgaggc acaggaattg ttctgattaa
    20581 aaaggcgctt cggcgccttt tcagttggct gacaaagtgc atttgtttat gccggatgcg
    20641 ggtgaacgcg ttatccggct aacaaaatcg tgaaaaatca atatattgca ggaaacccat
    20701 aagcctgata agcgttgtgc atcaggcaaa cttcacgcat ttacactcgc ccctgccctt
    20761 tcaaccattc gcgcacgagg aacagcgcgc tgacattacg cgcttcgttg aagtcagggt
    20821 cttccagcaa atccatcata tgcgccagcg gccagcgcac ctgtggtagc ggctctggct
    20881 catcgccttc cagtgattcc gggtagagat cttgcgctac cacgatattc attttgctgg
    20941 aaaaataaga cggtgccatg ctgagcttct tcaaaaaagt cagatcgttc gcaccaaatc
    21001 caacctcttc ttttagctcg cggttagcgg cttcgtagac gctttcaccc ggatcaatta
    21061 aacctttcga aaaacctaat tcgtaggatt cagttcccac tgcgtattcg cggatcagga
    21121 tcaggtgatc gtccacaatc ggcacaatca tcactgcttc ccggttggtt ggacgcattc
    21181 gttcataaac acgccgcacg ccattgctga actccagatc cacgctctcg acggtaaaca
    21241 gtcgggaacg ggctacagtt tcaacattca gaatggtggg tttttgtaat gatttgctca
    21301 tcgtgggatc tatgctgtga aatcagcggt tattgtgcga tatcggacac gctttcggca
    21361 atgtgaattg catgttattt acatttatgt aacttaataa ataatcgtcc tcaaatcaaa
    21421 ttaaaagtca ataggttgaa ataactccag gaatttgctg atattccgcc ttcagagggt
    21481 ttgctatgat cagcggttac tgtgatgtgc ttaatgatgc tcaagttaaa ctccacgctt
    21541 gccgatagcc aaccgcagaa tcatgtattg tgtctggtgc gactgaccac gcctgacaga
    21601 ctaagtaaga tggggaaagc atgagcacca ttgtgatttt tttagctgct ttgctggcct
    21661 gctcactact tgcgggatgg ctgataaaag tgcgatccag acggcgtcag ctgccctgga
    21721 ccaacgcctt cgcggatgcg caaacgcgta aactcacacc tgaagaacgt agcgccgttg
    21781 aaaattatct tgagagcctg acgcaggtat tacaggtgcc tggcccaacg ggagctagcg
    21841 cggcaccgat ctctctggcg ctgaatgccg aaagcaacaa cgtcatgatg ctgacacacg
    21901 ctatcacgcg ttacggcatc tctaccgacg atccgaataa atggcgttac tacctcgatt
    21961 cggtagaagt ccacctgccc cctttctggg aacaatacat caacgatgag aataccgttg
    22021 aactgattta taccgattcg ctgccgctgg ttatttcact caacggtcat acgctgcagg
    22081 agtacatgca ggaaacgcgc ggctatgcct tgcaacctgt tccgtccacg caggcgtcga
    22141 ttcgcgggga agagagtgag caaatcgagc tactcaatat tcgcaaagaa acgcatgaag
    22201 aatatgcgct gagtcgtccg cgcgggctgc gtgaagcgtt gctgatcgtc gcctccttcc
    22261 tgatgttctt tttctgcctg attaccccgg atgtatttgt tccgtggctg gcaggcggcg
    22321 cattactgct gctgggcgca ggtctgtggg ggctattcgc gcccccggca aaatcctccc
    22381 tgcgagaaat tcattgtctg cgcggtacac cccgtcgttg gggattgttt ggcgaaaacg
    22441 atcaggaaca gatcaacaat atttcactcg gtattatcga cctggtctat cccgcacact
    22501 ggcagccata cattgctcag gatctcggtc aacaaaccaa tattgatatc tatctcgacc
    22561 gccacgtagt gcgtcagggg cgatatcttt cgctgcatga tgaagtaaaa aactttccgt
    22621 tacagcactg gctgcgcagt acgattatcg ctgcgggctc gctgctggtg ctgtttatgc
    22681 tgttattctg gatcccgctg gatatgccgc tgaaattcac tctctcatgg atgaaaggcg
    22741 cgcagaccat tgaagccacc agcgtaaaac aactggctga cgctggcgtg cgggtaggcg
    22801 atacattgcg tattagcggt acgggaatgt gtaatattcg cacttccgga acctggagcg
    22861 cgaaaaccaa ttcacctttt ttaccgtttg actgctcgca gatcatctgg aatgacgccc
    22921 gctcattacc attaccagaa tctgaactgg tcaacaaagc gacggcattg actgaagcag
    22981 ttaatcgcca gctgcaccct aaaccggaag atgaatctcg cgtcagtgcc tcattacgtt
    23041 cagcgattca aaaatccggc atggtattgc ttgatgattt tggcgacatt gtactgaaga
    23101 cagcggattt atgttctgcc aaagatgact gtgtgcgact gaaaaatgcg ctggtcaatc
    23161 tcggcaacag taaagactgg gacgcgctgg taaaacgcgc caacgccggg aagctcgatg
    23221 gcgtgaatgt gttattacgc ccggtgagtg cggaatcgct ggataacctg gtggcaacct
    23281 ccaccgcgcc gtttatcacg catgaaacgg cgcgagcggc acaatcacta aacagtccgg
    23341 cccccggcgg attcctgatt gtcagcgacg aaggcagcga ttttgttgat cagccctggc
    23401 cttcggcatc actttacgac tacccgccgc aagaacagtg gaacgctttc cagaaactgg
    23461 cacaaatgct gatgcatacg ccgtttaacg ccgaaggtat cgtcacaaaa atcttcactg
    23521 acgccaatgg tacgcagcat attggccttc atccgatccc ggatcgttcc ggcctgtggc
    23581 gctatctcag caccacattg ctgctactga cgatgctggg tagcgccatt tacaatggcg
    23641 tacaggcctg gcgtcgttac cagcgtcatc gcactcgcat gatggagatt caggcctatt
    23701 atgaaagctg cctgaacccg caactgatca ccccttcaga aagccttatc gaataacacg
    23761 tttgcgcggc aggttatgct accctgtcgc gcaaattgct tcactctgga gatttccctc
    23821 atgcatatca acattgcctg gcaggacgta gataccgttc tgctggatat ggacggcaca
    23881 ttgctcgacc tcgccttcga taactatttc tggcaaaagc tggtgcctga aacatggggc
    23941 gcgaaaaacg gggttacgcc acaggaagcg atggaatata tgcgccagca atatcacgac
    24001 gtacagcata cgctaaactg gtactgtctt gattactgga gtgagcaact gggtctggat
    24061 atctgtgcga tgaccaccga gatgggaccg cgtgccgtac tgcgtgaaga taccattccg
    24121 tttcttgagg cactgaaagc cagcggtaag cagcgaattt tgctcaccaa tgcgcatccg
    24181 cacaacctgg cggtaaaact tgagcatacc ggtctggacg cacaccttga tttattactt
    24241 tccacccaca catttggtta tccgaaagag gatcagcggt tatggcatgc ggtggccgaa
    24301 gctacgggtc tgaaagctga aagaacgctg tttattgatg acagcgaagc gattctcgat
    24361 gctgccgcgc aatttggtat tcgttactgc ctcggcgtga ctaatcctga ttccgggatt
    24421 gccgagaaac agtatcaacg ccatccgtca ctgaatgact accgccgcct gatcccctcg
    24481 ctaatgtgaa ggagacgcca tgaaagaaaa acctgctgtt gaggttcgac tggataaatg
    24541 gctatgggct gcccgttttt ataaaacccg cgcgctggcc cgtgaaatga ttgaaggcgg
    24601 taaggtgcat tacaacgggc agcgcagcaa gccgagcaaa atcgtcgagc tgaatgccac
    24661 gctcactctg cgccagggaa atgacgaacg cacggtgatt gtaaaggcga ttactgaaca
    24721 gcgtcgcccc gccagcgagg cagccttgct gtatgaagag actgcggaaa gtgtagagaa
    24781 acgcgaaaaa atggcgatgg cacgtaaact taatgcctta accatgccgc acccggaccg
    24841 acgcccggac aaaaaagagc gccgcgacct gttacgattt aaacacggcg acagtgaata
    24901 actgtcacct gcaagagaga tgattatgcc gcaacatgac caattacatc gctatctgtt
    24961 tgaaaatttt gccgttcgcg gcgaactggt aaccgtttcg gaaaccctgc aacagatcct
    25021 tgagaaccac gattatccgc agcccgttaa aaacgtgctg gcagaactgc tggttgcgac
    25081 cagcctgtta accgctacgc tgaagtttga tggtgatatc accgtacagc tgcaaggcga
    25141 cggtccgatg aatctggcgg ttattaacgg taacaataac cagcaaatgc gcggtgtggc
    25201 gcgcgtgcag ggcgaaattc cagaaaatgc cgacctgaaa acgctggtcg gcaatggtta
    25261 cgtggtgatc accattaccc cgagcgaagg cgaacgctat cagggcgtgg ttggtctgga
    25321 aggtgatacc ctggcggcct gcctggaaga ttactttatg cgttctgaac agctgccgac
    25381 gcgcctgttt attcgcaccg gcgacgtaga cggcaaaccg gctgcaggcg gtatgttgtt
    25441 gcaggtaatg cctgcgcaaa atgcccagca ggacgacttt gaccacctgg cgacgctaac
    25501 cgaaaccatc aaaaccgaag aactgctgac cttaccggca aacgaagtgt tgtggcgttt
    25561 gtatcacgaa gaagaggtga cggtttacga tccgcaggat gtggagttca aatgcacctg
    25621 ctcgcgtgaa cgttgcgccg atgcgctgaa aacgctgcct gatgaagaag ttgatagcat
    25681 cctggcggaa gatggcgaaa ttgacatgca ttgtgattac tgcggtaacc actatctgtt
    25741 caatgcgatg gatattgctg aaatccgcaa caacgcgtct ccggcagatc cgcaagttca
    25801 ttaatgagta tgtccggcag agagggctct gccggatttt acgacgtaat ggcctgatgt
    25861 gctgcgctta ccaggcctgt gaaataaatc gcaaattatt gttggtcaga tacagcgtcg
    25921 tatccgttaa aagagttgtc acttacttaa gtttatttcc agcgtttcac catctaccgt
    25981 cagatcgcgc cacgctttcg gcctcgtccc cagttttcca tccttcgcag ctgtgagtaa
    26041 tttttctttc gttatttcgt tcggaagtaa actcatatca agcgcgtccc attcttttct
    26101 gtctgagtca aagccataga caatgactct gtcatcatta aaagcaaaca ggatccgctc
    26161 cgcctgacca tcactattca gatcttgctc gaccaggacg caggcgtctt tttcaataca
    26221 ggtcatcacg ttatagcgat cctggattaa ggccgaccag aatgtcgcat ccggtttaac
    26281 agaaccaggg gcaattaaca cattttcggc taatactttt tccgatacct gttcctgaag
    26341 atgttgctct ccatctaacg ccatcagcag atcccgcgcg cgtttcgggt ctttcataaa
    26401 cccggcatcg cttttcagcg actcaagcgc cgcacgtcca tagcgaccgc tctgttcgag
    26461 catgtagata gttacctggt ctggcgtgtt tttgccgctc tgataacgcg ccatatgact
    26521 gttcacgcta atacgcatac tgtccagcac cggcgaatta agaagcacca gtatcaccaa
    26581 caccaataac gaaaccgcaa ggttcacttt gccctgaaga acaaggggat tttgcccatt
    26641 acgccagacg atactgacaa aataccccag cgaccacacc agtaacacca gcaccgccag
    26701 cgcgccctgc aagcggtcga cagtccagcc gtactgagcg acccgcagcc ataacgccca
    26761 ggcggcgacg aacacataca gcggagcaac cagcaaagcg gttttaatca ggcaacgcaa
    26821 cggccccgtc cagggaagtg acgctttttg cggatcgcgg acaatagcca ttaagatcaa
    26881 ttgcaaaaag gccagcgtta acagcaaccc ggcggcggag atgtggcgag aaatcgcgct
    26941 caggcccgta aacggcagag tgatgataaa catcagggtt agcaatgata ctaacggcag
    27001 caaccccgtg gcgattaacg tgaacaactt ttgaatagag tcgattaaac gtgactgtgt
    27061 gcgcgcgaga atcaccgcca gtgcggtaac cagacctaat gtaagataca taaaccagtc
    27121 ggttgcaaaa aagagcgtat tgaaaaactt gatgccaaca agtttaaaca actcacccca
    27181 cagtaaaagc accagccacg tcaggccatt ggcgagaaaa atcaccagta atataagtac
    27241 gttatgccat actgactggt aaaagtagct atagcggcta ctgccattgc ggatacgaag
    27301 gctttgttgt atccacggta gcaacaacat tgccatcaac agcagatagc agccaaaatc
    27361 ccaaatcgcc ttttcagctc tccaggggtt catgccatca gtttgccact tcagccaacc
    27421 gctcatccct gtcgtggcaa taaacaccag cgccagccat ccccaaaggc gcttctgttt
    27481 aaaagagatc acggagaaga gtaaaacgga tgaaaacgca acggtcgcag gcacgccata
    27541 aacaatccaa ctgtgatttt ttccggcaag ccaggcgatc aacaggtagc aaaccagccc
    27601 ctgaagtaat ccggtagcaa tcatccccca gcgtgttgcc ggtgaaagtt caacgttatc
    27661 catgttggtt atccagaatc aaaaggtggg ttaattatcg catccgggca gtagtatttt
    27721 gcttttttca gaaaataatc aaaaaaagtt agcgtggtga atcgatactt taccgattga
    27781 atttgcatca atttcattaa ggaatgcgat tccactcaca atattcccgc catataaacc
    27841 aagatttaac cttttgagaa cattttccac acctaaaatg ctatttctgc gataatagca
    27901 accgtttcgt gacaggaatc acggagtttt ttgtcaaata tgaatttctc cagatacgta
    27961 aatctatgag ccttgtcgcg gttaacaccc ccaaaaagac tttactattc aggcaataca
    28021 tattggctaa ggagcagtga aatgcgcgtt aacaatggtt tgaccccgca agaactcgag
    28081 gcttatggta tcagtgacgt acatgatatc gtttacaacc caagctacga cctgctgtat
    28141 caggaagagc tcgatccgag cctgacaggt tatgagcgcg gggtgttaac taatctgggt
    28201 gccgttgccg tcgataccgg gatcttcacc ggtcgttcac caaaagataa gtatatcgtc
    28261 cgtgacgata ccactcgcga tactttctgg tgggcagaca aaggcaaagg taagaacgac
    28321 aacaaacctc tctctccgga aacctggcag catctgaaag gcctggtgac caggcagctt
    28381 tccggcaaac gtctgttcgt tgtcgacgct ttctgtggtg cgaacccgga tactcgtctt
    28441 tccgtccgtt tcatcaccga agtggcctgg caggcgcatt ttgtcaaaaa catgtttatt
    28501 cgcccgagcg atgaagagct ggcaggtttc aaaccagact ttatcgttat gaacggcgcg
    28561 aagtgcacta acccgcagtg gaaagaacag ggtctgaact ccgaaaactt cgtggcgttt
    28621 aacctgaccg agcgcatgca gctgattggc ggcacctggt acggcggcga aatgaagaaa
    28681 gggatgttct cgatgatgaa ctacctgctg ccgctgaaag gtatcgcttc tatgcactgc
    28741 tccgccaacg ttggtgagaa aggcgatgtt gcggtgttct tcggcctttc cggcaccggt
    28801 aaaaccaccc tttccaccga cccgaaacgt cgcctgattg gtgatgacga gcacggctgg
    28861 gacgatgacg gcgtgtttaa cttcgaaggc ggctgctacg caaaaactat caagctgtcg
    28921 aaagaagcgg aacctgaaat ctacaacgct atccgtcgcg atgcgctgct ggaaaacgtc
    28981 accgtgcgtg aagatggcac tatcgacttt gatgatggtt caaaaaccga gaacacccgc
    29041 gtttcttatc cgatctatca catcgataac attgttaagc cggtttccaa agcgggccac
    29101 gcgactaagg ttatcttcct gactgctgat gctttcggcg tgttgccgcc ggtttctcgc
    29161 ctgactgccg atcaaaccca gtatcacttc ctctctggct tcaccgccaa actggccggt
    29221 actgagcgtg gcatcaccga accgacgcca accttctccg cttgcttcgg cgcggcgttc
    29281 ctgtcgctgc acccgactca gtacgcagaa gtgctggtga aacgtatgca ggcggcgggc
    29341 gcgcaggctt atctggttaa cactggctgg aacggcactg gcaaacgtat ctcgattaaa
    29401 gatacccgcg ccattatcga cgccatcctc aacggttcgc tggataatgc agaaaccctc
    29461 actctgccga tgtttaacct ggcgatccca accgaactgc cgggcgtaga cacgaagatt
    29521 ctcgatccgc gtaacaccta cgcttctccg gaacagtggc aggaaaaagc cgaaaccctg
    29581 gcgaaactgt ttatcgacaa cttcgataaa tacaccgaca cccctgcggg tgccgcgctg
    29641 gtagcggctg gtccgaaact gtaatgattt gaagctggag aatatctatc cagtatctta
    29701 tagaaagcaa aacgggaggc accttcgcct cccgtttatt tacccttctt ttgtcgtgcc
    29761 ctgcgcccgc gttaccggca ctggcagcca ggcgcgaatg gaaagcccgc cccgctcgct
    29821 ggtgccaagc tccagcatcc cgttatggtt atccacgata cgctgcacaa ttgccagccc
    29881 taatcccgtg ccgctaatgg tgcgcgcact gtcgccgcgg acaaacggct ggaacaggtg
    29941 cttacgttgt tccggcgcaa ttcccggacc gtcatcttcc acctggaacc aggcgcgatt
    30001 cggctccgtt ccgctgctga ctttgatcca gccattgcca taacgggcgg cgttgaccac
    30061 catattcgcc accgcgcgtt tgatcgacag cgggtgcatt ttcacttcaa tgctgccggg
    30121 gtaaagcgcg gtttcaattt cccgctcata gccactttcg gcagcaatca cctcaccgag
    30181 tactgcatta agatccgcca tttccatcgg catctcctgc ccggtgcgca ggtagtcgat
    30241 aaactgctca atgatggcgt tgcactcttc gatatcttta ttgatcgatt ctgccagata
    30301 gccatcctgc tcgctcatca tctcagtcgc caggcgaata cgcgtcagcg gcgtgcgcaa
    30361 gtcgtgactt acccccgcca tcagcagcgt gcggtcatcc gccagttgct taacaccagc
    30421 cgccatatgg ttaaaggcac gggtaacgga acgcacctcc gaagcgccat attcacgcag
    30481 cggcggcgga ataatccctt taccaacctg caaggctgcg tgttcgagat cgaccaacgg
    30541 tcggttctgg atacgaataa acagccacgc cccgcctatc gccaatagca taatcgccag
    30601 cgtatagcgg aacagcggag agaaatcgcc ctgatgaatt tcggtcagcg gcacgcgtac
    30661 ccagatattg ggcgacagcc aggttttcag ccagacgaca ggcgaacttt tgttgacctc
    30721 aacgcgcact tccgtcgggc cgcccagttg ctgtgccatc tgatggctta agaattcata
    30781 gtgttgcgcc caacgcagac ctgcctcttc ggcagcctcg ttggagtaga gagagatccc
    30841 cagctcacgg tagatctccc gacggaaagc gggaggcaca accaactgcg tgccgtcctc
    30901 cagttgcagt ttgtcggtca tcaacatacg cacttcgtac gcgaggactt tattaaactg
    30961 ctggaggctc ggcaaaatcg cgaagttcag caccaccaga taagtcgtca ccaggctggc
    31021 gaacagcaag gtgacgatga gcaataacgt acgggcaaat gaacttcgtg gcgagaagcg
    31081 caatcgcctc atgctttaga gccgtccggt acaaagacgt agcccaggcc ccaaacggtc
    31141 tgaatgtaac gcggatgcgc tggatcttct tctaccatgc ggcgcagacg cgaaatctgc
    31201 acgtcgatgg agcgttccat tgcggaatat tcacgaccac gggcaaggtt catcagctta
    31261 tcgcgggaga gcggctcacg cggatggctg accagtgcct tcagtaccgc aaactcaccg
    31321 ctggtgagcg gcatcggctc atcttcgcgg aacatttcgc gcgtaccgag gttaagtttg
    31381 aacttaccga aagcaattac cgcctcttcc tgtgacggtg cgcctggcag ttcgttcgcc
    31441 tgacgacgca gcaccgcacg aatacgggcc agcagttcac gcgggttaaa cggttttgga
    31501 atgtagtcgt cagcgccaat ctccaggcct acgatacggt ccacttcttc ccctttcgcc
    31561 gtcaccataa tgatcggcat cgggttgctc tgactacgaa gacgtcggca aatcgacaag
    31621 ccatcttcac caggtaacat taaatccagt accataagat ggaaagattc acgagtcagc
    31681 aggcgatcca tctgttctgc attagcgacg cttcgaacct ggaagccttg ttcggtgaga
    31741 taacgttcca gcagcgcacg caggcgcatg tcgtcatcga ccaccagaat cttgtagttc
    31801 tcttgcattg tttgtactcc caaaggttcg caacaatttg taagcgtgta ttcttaaaaa
    31861 agctcacgtt cgtcaccagc taaatctggt atgaatttca gcctaaattg ttacaaagca
    31921 tattaaacag cagcttaagt atacaattta ttcggcgaaa cattattgat tctgttgata
    31981 tgatcacgtt atacccaatg tgcgcattat caaacagaca aagggaatca acgagatgaa
    32041 aacgcccctg gttacccggg aagggtatga aaaactcaaa caagagctta attatctctg
    32101 gcgtgaagaa cgcccggagg tcacaaaaaa ggtgacctgg gccgcaagtc tgggcgaccg
    32161 cagcgaaaat gctgactatc agtataataa aaagcgtctg cgtgaaatcg accgtcgcgt
    32221 gcgctatctc actaaatgca tggaaaatct caaaatcgtc gattactccc ctcagcagga
    32281 aggcaaagtc ttttttggcg cgtgggtgga gattgaaaac gacgatggcg tgactcaccg
    32341 tttccgtatt gtcggctacg atgaaatttt tggccgtaaa gattacatct ctatcgattc
    32401 cccgatggcc cgcgcattgc tgaaaaaaga agtcggcgat ctggcggtgg tgaatacccc
    32461 tgccggggaa gcgagctggt atgttaatgc tatcgagtac gtgaaaccgt aaggaagagt
    32521 cttaacctcc tgccgatggc tggcattttt gccagccagt ccgtataact atcccctgat
    32581 ttttgatccg aaaagatgaa ctcaaaccat gatgaatgat tcgttctgcc gcattattgc
    32641 gggtgaaatt caggcgcgcc cggaacaggt tgacgctgcc gttcgcctgc ttgacgaagg
    32701 gaataccgtg ccgtttatcg cacgttatcg taaggaaatc accggcggtc tggatgacac
    32761 gcagctgcgt aatctggaaa cgcgtctgag ctatctgcgc gagctggaag agagacgtca
    32821 ggcgatcctc aagtccattt ccgagcaagg caaactcacc gatgatctgg cgaaggccat
    32881 caacgccacc ctaagcaaaa ccgaactcga agacctctac ctgccctaca aacctaaacg
    32941 ccgcacccgc gggcaaatcg ccattgaagc agggcttgag ccgttggctg acctgctgtg
    33001 gagcgatccg tcacacacgc cagaagtcgc cgctgcacaa tatgttgatg ccgataaagg
    33061 cgtggcagat accaaagccg cgctggatgg cgcgcgctat atcctgatgg aacggtttgc
    33121 cgaagatgcc gcgctgctgg cgaaagtgcg tgattatctg tggaagaacg cgcatttggt
    33181 ttctacggtg gtgagcggta aagaagagga aggggcgaaa ttccgcgact atttcgatca
    33241 tcacgaaccg ttgtccacgg tgccttctca ccgcgcgctg gcgatgttcc gtgggcgtaa
    33301 cgaaggcgta ctccagcttt cgctgaatgc cgatccgcag ttcgatgagc cgcccaaaga
    33361 gagctattgc gagcaaatca tcatggatca ccttggcctg cgcctgaaca atgccccggc
    33421 ggatagctgg cgcaaaggcg tagtgagctg gacctggcgc atcaaggtgc tgatgcatct
    33481 ggaaaccgaa ctgatgggta ccgtgcgcga acgcgcggaa gatgaagcaa tcaacgtctt
    33541 tgcccgtaac ctgcacgatc tgctgatggc ggcccctgcc ggactgcgtg caacgatggg
    33601 cctcgatccg ggtctgcgta ctggggtaaa agtggcggtg gtcgatgcca ctggcaaact
    33661 ggtggcgacc gataccattt acccgcacac cggacaagcc gcaaaagcag cgatgaccgt
    33721 tgctgccttg tgtgaaaaac ataacgttga actggtggcg atcggcaacg gtacagcttc
    33781 ccgcgaaact gaacgtttct atctcgacgt gcagaagcag ttcccgaaag tgaccgcaca
    33841 gaaagtgatc gtcagcgaag ctggcgcgtc ggtttactca gcttccgagc tggcagcgca
    33901 ggagttcccg gatctcgacg tttcgctgcg tggtgcggta tctatcgccc gccgtttgca
    33961 ggatccgctg gcggagctgg tgaaaatcga tccgaaatct atcggcgtag gtcagtatca
    34021 gcatgacgtc agccagacgc aactggcccg caaactggac gcagtagtag aagactgcgt
    34081 aaacgccgtt ggcgtcgatc tcaacactgc ttctgttccg ctgttaaccc gcgtggcggg
    34141 cctgacgcgc atgatggcgc aaaacatcgt ggcctggcgc gatgagaacg gtcagttcca
    34201 gaaccgtcag caactgctga aagtgagccg tctggggccg aaagccttcg agcagtgcgc
    34261 gggcttcttg cgcattaacc atggtgataa cccgctggac gcgtctaccg tccacccgga
    34321 agcctatccg gtggtggaac gcattctggc agcaacacag caggcactga aagatctgat
    34381 gggtaacagc agcgaactgc gtaacctgaa agcgtctgac tttactgatg aaaaattcgg
    34441 tgtgccgaca gtaactgaca tcatcaaaga gctggaaaaa ccgggtcgcg atccgcgtcc
    34501 ggaatttaaa accgctcagt ttgccgatgg cgtcgagaca atgaacgacc tgcaaccggg
    34561 tatgatcctc gaaggtgcgg tgaccaacgt caccaacttt ggcgcgtttg tcgatatcgg
    34621 cgtgcatcag gacggcctgg ttcacatctc ttcattgtcg aacaagtttg tagaagatcc
    34681 gcataccgtg gtgaaagcgg gcgacattgt gaaggtgaaa gtgctggaag tggatcttca
    34741 gcgtaaacgt atcgccctga ccatgcgtct ggacgagcag cctggcgaaa ccaacgcccg
    34801 tcgcggcggc ggtaatgaac gcccgcaaaa caaccgcccg gcagccaaac cgcgcggacg
    34861 tgaagcgcag cctgccggta acagcgcgat gatggacgcg ctggcggcag caatgggcaa
    34921 aaaacgttaa acgccctgat tgacccctaa acctgcgcga taaggtgtcc acgccgcatc
    34981 tggcaacgaa cgccttgtct gacataaaag tgccggagaa tatctccggc atttttattc
    35041 cacagccaaa ctcacaatat attccggcaa catttatcac ttcattaaca actgaaacct
    35101 taattaaaca ttagccagtc ctggtaattc gctattcgaa ttatattttc gctgcgatat
    35161 aaccttgagc cacatcaaca ttgagtcaga ttattattca aaccaacatt cgcacacatt
    35221 ttaagtattg ctgatagaaa ccattctcat tatcattgtg ctgttgatta tttaatctct
    35281 ccttcgttgg caaatcatct ggtctcatgt cgctgtcaaa cgccccatga ggtagttatc
    35341 cagttaatga gaaacaagta ggcacctatg caatacactc cagatactgc gtggaaaatc
    35401 actggctttt cccatgaaat cagcccggca tatcgccaaa aactgctttc tcttggcatg
    35461 ttacctggct cctcttttaa tgtggtgcgc gtcgctccac tcggcgaccc cattcatatc
    35521 gaaacccgtc gtgtgagcct ggtattacgc aaaaaagatc tggccttatt agaagtggaa
    35581 gcggtttcct gttaatacgg tgataacaac aatgaaaaaa ttaaccatag gcttaattgg
    35641 taatccaaat tctggcaaga caaccttatt taaccagctc actggcgcac gtcagcgtgt
    35701 aggtaactgg gctggcgtta ccgtcgaacg taaagaaggg caattctcca ccaccgatca
    35761 tcaggtcacg ctggtggacc tgcccggcac ctattctctg accaccatct catcgcagac
    35821 ctcgctcgat gagcaaatcg cctgtcacta cattttgagt ggcgacgccg acctgctgat
    35881 taacgtggtg gatgcgtcta accttgagcg taacctgtac ctgacgctac aactgctgga
    35941 actcggcatt ccctgcatcg tggcactgaa catgctcgac attgccgaga agcaaaatat
    36001 tcgtattgaa attgatgctc tgtcggcgcg tcttggctgc ccagtgatcc cgctggtttc
    36061 aacccgtggt cgcggtattg aagcgctcaa gctggcgatt gatcgctata aagctaacga
    36121 gaatgtggaa ctggtgcatt acgcacagcc gctactcaac gaagcagatt cactggcaaa
    36181 agtgatgcct tccgacatcc cgctgaaaca acgtcgctgg ctgggcctgc aaatgctgga
    36241 aggcgatatc tacagccgcg cctacgccgg tgaagcgtcg cagcatctgg atgccgccct
    36301 cgcccgtctg cgtaatgaga tggacgatcc ggcgctgcac attgccgatg cgcgttacca
    36361 gtgcattgct gccatctgtg atgtggtaag caacaccctg acggcagaac ccagccgttt
    36421 caccactgcg gtagataaaa tcgtgctcaa ccgtttcctc ggtctgccga ttttcctctt
    36481 tgtgatgtac ctgatgttcc tgctggctat caacatcggc ggggcgttac agccgctgtt
    36541 tgacgtcggc tccgtggcgc tatttgtgca tggtattcaa tggattggct acacgctcca
    36601 cttcccggac tggctgacta tcttcctcgc ccagggcctg ggtggcggca ttaacaccgt
    36661 gctgccactg gtgccgcaga ttggcatgat gtacctgttc ctctccttcc ttgaggactc
    36721 cgggtatatg gcgcgtgcgg cgtttgtgat ggaccgtctg atgcaggcgc tgggcttgcc
    36781 ggggaaatcc tttgtgccgc tgatcgtcgg tttcggttgt aacgtaccgt cggtaatggg
    36841 tgcacgtacg cttgatgcac cgcgtgaacg tctgatgacc atcatgatgg caccgtttat
    36901 gtcctgcggc gcgcgtctgg ctatcttcgc agtattcgcg gctgccttct tcgggcagaa
    36961 cggtgcgctg gcggtcttct cgctgtatat gctgggtatt gtgatggcgg tgctgactgg
    37021 cctgatgctc aagtacacca tcatgcgcgg tgaagcgacg ccgtttgtca tggagttgcc
    37081 ggtctatcat gtaccacacg ttaaaagcct gattatccag acctggcagc gtctgaaagg
    37141 cttcgttctg cgtgctggta aagtgatcat catcgtcagc attttcctga gcgctttcaa
    37201 cagcttctcg ctgagcggga aaatcgtcga taacatcaac gactcggcgc tggcgtccgt
    37261 cagccgggtg atcaccccgg tcttcaagcc aattggcgtg catgaagata actggcaggc
    37321 aacggttggc ctgtttacag gtgccatggc gaaagaagtg gtagtgggta cgctcaacac
    37381 cctctacacc gcagaaaata ttcaggacga agagttcaat ccggcagaat ttaacctcgg
    37441 tgaagagctg ttcagtgcga tagatgaaac ctggcagagc ctgaaagaca ccttcagcct
    37501 tagcgtactg atgaacccca ttgaagccag caaaggcgac ggcgaaatgg gtaccggggc
    37561 gatgggcgtg atggatcaga aattcggtag cgcagcagca gcttacagct acctgatttt
    37621 cgtcctgctg tatgtaccat gtatctcggt gatgggagcc atcgcccgtg aatcaagccg
    37681 tggctggatg ggcttctcca tcctgtgggg gctgaatatc gcttactcgc tggcaacatt
    37741 gttctatcag gtcgccagct acagtcagca cccaacttac agcctggtgt gcattctggc
    37801 ggttatcctg tttaacatcg tggttatcgg tctgctgcgc cgcgcgcgta gccgggtgga
    37861 tatcgaactg ctggcaaccc gcaagtcggt aagcagttgc tgcgcagcca gcaccaccgg
    37921 tgattgccat taatggcttc acttattcag gtgcgcgatt tgctggcgtt acggggccgt
    37981 atggaagcgg cccagataag ccagacattg aacactccac agccaatgat taacgccatg
    38041 ctgcaacaac tggaaagtat gggcaaagcc gtgcggattc aggaagaacc tgacggctgc
    38101 ctctctggca gttgtaaaag ctgcccggaa ggaaaagcct gtctgcgcga gtggtgggcg
    38161 ctgcgttaac cttactccat cgcctgtttt ggaaagcccg gtatgcgtct gcatccgggc
    38221 ttttttgcgt gcggctttcc ataaaaatgc aactcttgca gcacggcgta agttcctttg
    38281 aaagcatctc gcagggatga aaactcgcta atgcacagat gtggagtggc gcgtagagtc
    38341 gcggcattca aacaacaggt gaaggaacgc catgagtaaa aagcagagtt ccaccccaca
    38401 tgatgcgctg ttcaaactct ttttacgcca accggacacg gctcgcgatt ttcttgcgtt
    38461 ccatttaccg gcccccattc acgcgctctg tgatatgaaa accctcaagc tggagtcgag
    38521 cagctttatt gatgacgatc tgcgtgaaag ctattccgat gtgctgtggt cggtgaaaac
    38581 ggaacaagga ccaggataca tctattgtct gattgaacat caaagcacct caaacaaact
    38641 gatcgcattt cgcatgatgc gttacgctat tgccgcaatg caaaatcacc ttgatgctgg
    38701 atacaaaacg ttgccgatgg tggtgctatt gttgttttac cacggtattg aaagccccta
    38761 tccctattcg ctgtgttggc tggattgttt cgccgatccc aaactggcaa ggcagcttta
    38821 tgcctccgca tttccgctga ttgatgtcac cgtcatgcct gatgatgaaa tcatgcagca
    38881 ccgacgtatg gcgctgctgg agttaattca aaaacatatt cgtcaacgcg acctgatggg
    38941 gctggtagag caaatggcct gcttattaag tagtggatac gctaatgaca gacaaatcaa
    39001 agggctgttt aattacatac tgcaaaccgg cgacgcggta cgttttaacg attttatcga
    39061 cggcgttgcc gaacgttcac cgaaacacaa ggagagttta atgacaattg cggaaagatt
    39121 gcggcaggag ggggaacaat ccaaagccct gcatatagcc aaaataatgc ttgaatccgg
    39181 agtccctctt acagacatca tgcgctttac cggggtgtca gaagaagagt tggctgcggc
    39241 gagccagtaa atttctgtct cgccatttca aaagttgcct acaccctctg cttcaacgcc
    39301 accagcaggt gacaaaactc gaccggatgc gaaataaacg gcgcatgggc cgctttggcg
    39361 aagatatatg attcgctgtg aggccaaagt ttatccagca tcggcaccac tttgcgcggc
    39421 accagaccgt cgagatagcc atacaatcgc aaaaacggca tgggcacgtt ttgcagcggc
    39481 tgacggagat cgaccgtttt caggatttcc agcccgccat taagcacgtc aacctccggc
    39541 atcggtaacg ccagaacggt tttcttcaac gcccgcgcat cctggcgcgc cgtttcagtc
    39601 cccatggttt gtaacgccag gaaccgctcc actgtacgct gaaaatcatc actgagttgc
    39661 tgctgaaatc ccgccagcac gtccggtttt atccccggcc actcgtcacg agcactaaaa
    39721 caaggtgacg acgccacggt gaccagcgcc tgaacacgct cgggatgggt taacgcaatc
    39781 tggcttgcca ccagcccgcc cagactccag cctaaccaaa tggctttatc aggtgcctgt
    39841 tgcagcacgg cttcggccat atcagcaagt gacagcgcac caaatccccg gctacgcccg
    39901 aagccgggca ggtcaacaag gtgcagcgta aaatgcgagc taagttcctc gtcaatgcaa
    39961 cgccacactt cggcattcag tccccatccg tgcagcagca caagatgaac attcccctga
    40021 cctttggtct gccaccagat gttattcatc cgctattgtt ctcttttgac ttacaaggat
    40081 gaacatatgc taacagtacc gggattatgc tggctatgcc gaatgccact ggcgttaggt
    40141 cattggggga tttgttcggt ctgctcacgc gctgcccgta cagataaaac gttatgtcca
    40201 caatgtggat tacccgccac acactcacat cttccctgcg gtcgctgcct gcaaaaacca
    40261 ccgccctggc aaagactggt cacggttgcc gactatgcgc cgccgttaag tccgcttatc
    40321 caccagctta aattttcccg gcgcagtgaa atcgccagcg ccctgtcacg tctgttacta
    40381 ttggaagtct tacacgctcg tcgcaccacc gggttgcaat tgccggatcg catcatcagc
    40441 gttccgttat ggcagcggcg tcactggcgt cggggattta atcagagcga tttgctgtgt
    40501 cagccgttat cacgctggtt gcactgccaa tgggatagcg aagccgtcac acgtacacgg
    40561 gccactgcga cccagcattt tctcagtgcc cggctgcgca agcgcaacct gaaaaatgcc
    40621 tttcgtcttg aattgcccgt gcaaggtcgc catatggtga ttgtggatga tgtcgttacc
    40681 accggaagta ccgtcgcaga gattgcgcag ttgcttttac gcaatggtgc ggcgactgtc
    40741 caggtctggt gcctttgtcg aaccttgtag agcctcgatg atgggcgtat tataaccaac
    40801 taaaatagtc aactattagg ccattactat gatccgtatt tccgatgctg cacaagcgca
    40861 ctttgccaaa ctgctggcaa atcaggaaga agggacacaa atccgcgtat ttgtgattaa
    40921 ccctggcacg cctaacgctg aatgtggtgt ttcttattgt ccgccggatg ccgtggaagc
    40981 caccgacact gccctgaaat ttgacctgct gaccgcgtat gttgatgagt taagcgcacc
    41041 atacctggaa gatgcagaga tcgactttgt taccgaccag ttgggttctc agttaacgct
    41101 gaaagccccg aacgccaaaa tgcgtaaagt ggcagacgat gcaccgctga tggagcgcgt
    41161 ggagtatatg ctgcagtcgc agatcaaccc acagcttgct ggtcacggtg gtcgcgtttc
    41221 gctgatggaa atcacagaag acggttacgc cattctgcaa tttggcggcg gctgtaacgg
    41281 ttgttccatg gtcgatgtga cgctgaaaga agggatcgag aagcagctgc tgaacgaatt
    41341 cccggagctg aaaggtgtgc gcgatctcac cgaacaccag cgcggcgaac actcctacta
    41401 ctaagttatc ccctcttttg tagattgccc gatgcgacgc taaagcgtca tttcgggcct
    41461 acgttgaaca tgtgccagac gttggcacag cattgctttg gcaaatccct cccttcgttt
    41521 ccccgcgata atatgaccaa cctctcataa tttaaattta ccccgctctg gtgattctca
    41581 aacgccagat gttacccgta tcattcacat gggtaccaaa catactcctg acatctgact
    41641 acaataatta gttttagtgg gtatcagtcg tggtgccgca atatctctgt tcccgattgg
    41701 gataattaga gtttgtcgtc agaaaattga cgttacccat aacaaatgaa aggccaggta
    41761 aatcatgcca ttagtcattg ttgctatcgg tgtaatcttg ttgttgctcc tgatgatccg
    41821 cttcaaaatg aacggcttca tcgctctcgt cctcgtggcg cttgctgttg gattaatgca
    41881 aggaatgccg ctggataaag ttattggctc catcaaagcc ggtgtcggcg ggacgctcgg
    41941 tagccttgcc ctgatcatgg gttttggcgc aatgctgggc aaaatgctgg cagactgcgg
    42001 tggcgcacaa cgtatcgcca ccacgctgat tgccaaattt ggtaaaaaac acatccagtg
    42061 ggcggtggta ctgaccggtt ttaccgttgg ttttgccctg ttctatgaag tgggctttgt
    42121 gctgatgctg ccgctggtgt ttaccatcgc ggcttctgcg aatattccac tgttgtatgt
    42181 tggtgtacca atggcagctg cactgtctgt gacccacggc ttcctgccac cgcatccggg
    42241 tccgactgcg attgccacca ttttcaatgc cgatatgggt aaaaccctgc tgtacggtac
    42301 tattctggca atcccgaccg tgattctcgc cggtccggtt tacgctcgcg tgttgaaagg
    42361 tatcgataag ccaattccgg aaggtctcta cagcgcgaaa accttcagcg aagaagagat
    42421 gccgagcttt ggcgtcagcg tctggacttc tctggtgccg gttgtgctga tggcgatgcg
    42481 tgcgattgcc gaaatgatcc tgccgaaagg tcacgctttc ctgccggtag cggagttcct
    42541 cggtgacccg gtaatggcaa cgctgattgc cgtgctgatt gcgatgttca cctttggtct
    42601 gaaccgtggt cgttcaatgg atcagattaa cgacacgctg gtttcttcca tcaaaatcat
    42661 tgcgatgatg ctgttgatca tcggtggtgg cggtgcgttc aagcaggtgc tggtagacag
    42721 cggcgtggac aaatacattg cttccatgat gcacgaaacc aacatttctc cgctgctgat
    42781 ggcctggtcg attgctgccg tactgcgtat cgcgctgggt tccgcaaccg ttgcggcaat
    42841 cactgcgggt ggtatcgcgg caccgctgat tgcaacgacg ggtgttagcc cagagctgat
    42901 ggttattgcg gtgggttccg gtagtgtgat tttctctcat gtgaacgatc cgggcttctg
    42961 gctgttcaaa gagtacttta acctgactat cggcgagacc atcaaatcct ggtcgatgct
    43021 ggaaaccatt atctcggtgt gcggtctggt aggctgtctg ctgctgaata tggtgatttg
    43081 ataacactga ctgccggatg catcgcatcc ggcatcaacc gcactctact tcttcttcgc
    43141 tgcagctctg cgccgtctgt ccaaatcctt cagcaacttg ttcacgccat catcggcaaa
    43201 catcgtctca agcgttgcgg aaagcttgcg tcgccagttt ttatactggt aactggtgcc
    43261 aggaatattc accggttcgg ccatatccag ccagtcttcc ggctgtagtc ctaacagagc
    43321 actgttactg tcggcaatgt agcgctgcaa accacggttc agcgtcggcg tcatcgacat
    43381 caacgatgcc ttatgcccgg cacgttttgg cagacaacca tatttatgca gtgcatccag
    43441 cagcccttgc ttcgccagtt cgcgatcctg atacagaccg cgcagtacca cttcatccgg
    43501 atacagcccc agggttttgc ccagcgttag atccccgctc tcccagtaac cgcgtagcgt
    43561 aggcaggtca tgtgtcgccg caaccgccat cgactgctcc ggatacgctt tcggtgcacg
    43621 gaacgtcttc tcgtggtcgt tttcgaaata gagcactttg taggagtaca caccgctgct
    43681 gcgcagctta ccgacaatct ctaccggtac ggtaccgaga tcttcaccaa tcaccataca
    43741 gcgatgacgt ttactttcga gtgccagaat cgagagcaga tcatccaccg gatagtgaac
    43801 atacgcgccc tgatctgccg tctcgccata cggtatccac cacaaacgca gcatcgacat
    43861 cacatggtca attcgtaatg cgccgcagtt ttgcatattg gcacgcaaca gctcgataaa
    43921 cggttcatag gcacgcgcgg tgatgatatg cgggtccatt ggcggtaatc cccagttctg
    43981 ccccaacggg ccgaggatat ccggcggcgc gccaaccgac gctttcaggc aatatagttc
    44041 acggtcacac caggtttccg ccccaccttc cgctacgcca accgccagat cacgatacaa
    44101 gccaatcggc atttcatagc cctggcttat ctcccagcag gcggcaaact ggctgtaagc
    44161 cagccactgc aaccagagat aaaaatcgac gtcattacga tgttcttcgc agaactgacg
    44221 cacttctggt gaatccacgt tctgatacat ctctggccat gcaggccagc cccagcgcat
    44281 ttcgtcctct ttcacttgct gggcatgtag cgcatcaaag gctgcctgcc agaacaggct
    44341 gtcgccctgc tctgcaacaa actggcgaaa cgcggtcatc tgctcatcat cacgttgcgc
    44401 gaaacctttc cacgccattc gtaatgctgt catttttagg gcggtaaccg tggagtaatc
    44461 gacccagtcg gcatcgcgcg cctgttgcag cgtctgttgc gtggtcggca actgccacca
    44521 ggcctgagcc tcttcgctaa gatggaaatc ttcaacggcg ttaacgtcga tataaatcac
    44581 attcagccaa cggcgagaag acgggctgta tgggctggca ctctccggat ttgccggata
    44641 gagcgcatga atcgggttca ggccgatgaa cgacccgcca cgttttgcca catccaccag
    44701 catcgctttg agatcgccaa aatccccaat accccagttt ttttccgatc gcagcgtata
    44761 aagctgaacg caggcacccc acagcttttg tttattcagc aacgcctgcg gttcgtaaca
    44821 gcgtttcggg gcgacaatca cccggcaatg cgcgcgctgg tcgtcctggg tgagtgtcag
    44881 cgtgtgataa ccttccggca gcttcgtcgg tagattgaac gctttgcccc ccgttacatg
    44941 gcctttgtac tgtgttcctt cttcggtggt cagcagccag ctatattcgc cgctgccctc
    45001 caccaccatc ggcatttttt tgccgctggt ataaaccatg acattcggga ctggcgttac
    45061 cgccactttc gtggcggtac gttgatgcat cgcgtcaagc aaacgccgtt tggtttcggc
    45121 gctaatcgac tgcggtttac cgtgggcatt gatgtaattg gggctaatcc ccgccgccag
    45181 cgcggcatta tccagacgtt tgctttccat cgagcttcct tagcgttttg cctgccagat
    45241 acgagcctga taatcgcgaa tagagcgatc cgagctaaac ataccgcagc gggcggtatt
    45301 gaggatcgcc gcgcgagtcc aggcctcctg gtcgcggtac agcacatcca cctgcttttg
    45361 tgcctcaaca taggctgcga aatccgccat caccagatac ggatcgccgc cttgtttgcc
    45421 gatactgtgc agcatctggt cgaaggcatg cttatcgccg tcgctgtatt taccgctttc
    45481 cagctctttc aatactgcgt ccagcacctt atctttcttc cgccatttca ccgggtcgta
    45541 gcctttggcc agaatagcct tcacttgttc cacggtatga ccaaaaataa agatattttc
    45601 ttcaccgact ttctcggcga tttcaacgtt cgcaccatcc agcgtaccga cagtaagcgc
    45661 accattgagc gccagtttca tattgccggt accggaagct tctttacctg cagtcgaaat
    45721 ttgttcggag atatccgccg ccgggatcag tttttccgcc gccgaaacgc aataatccgg
    45781 caggaacacc accttcaact tatcgccaac cagtggatcg ttgttgatca cgtcagccac
    45841 tttgttgatc gcaaagataa tattcttcgc caggtagtag cccggtgccg ctttcgcgcc
    45901 gaagaggaag acgcgcggta cgcgatcagc ctgcgggttt tcacgaattt ctttgtacaa
    45961 cgccagaata tgcagcagat tcaggtgctg gcgtttgtac tcatgcagac gtttgatctg
    46021 aatatcgaaa atcgcctgtg gattgatctc aataccggta cgaactttca caaactccgc
    46081 cagacggact ttattcgcct gcttgatctc gcgatattgc tgacggaatt tcgcatcatc
    46141 agcgaatttt tccagattga tcagctgatc gagatcgtta gcccactctt tttgcagtga
    46201 tttatccaac agagccgcca gtgcagggtt gcactgtttg atccagcgac gtggggtaat
    46261 accgttggtg acgttatgga atttgttcgg ccatagctgg tgatattccg ggaacagatc
    46321 tttcaccacc agatccgagt gcagcgccgc aacaccgttc accgcgaaac cgccaaccac
    46381 acacaggttc gccatatgca cttgtttatc gtgcaccacc gccagtttgg cccacacttt
    46441 ttcatcgccc ggccaggttt tctccaccag cgttttaaag cgagtattaa tttcgttaat
    46501 aatctgcata tggcgcggca gtaagccttt caccagtttc acatcccagc gttccagcgc
    46561 ttctggcatc agggtatggt tggtgtaagc gaaagttttg ctggtaatgg cccaggcgtc
    46621 atcccagctc atctggtgct catcgatcag cacgcgcagc agttctggaa tcgcgatagt
    46681 tgggtgggta tcgttcagct gaataacttc gtaatccgcc agttcgtgca gtttacgccc
    46741 cgccagatga tggcgacgca aaatatccgc taccgaacag gcacactgga agtactgctg
    46801 catcaagcgc agctttttac cggcagtatg gttgtcgttt ggatagagaa ctttggtcag
    46861 tttttccgca ttgatgccct gctgttcggc acgcaggaaa tcaccgtcgt taaatttagt
    46921 cagatcaaac ggatgcgcgt gcgtcgcctg ccacagacgc agcggctgcg ccacgccatt
    46981 acgatagccg acaacgggga gatcccacgc ttgaccggta atggtaaact ccggctccca
    47041 gcgtccgtct ttcgtcactt taccgccaat ccctacctgc acatccagtg cttcgttgtg
    47101 gcggaaccac gggtagttac tgcgatgcca gtcatccggc gcttcaacct gtttgccatc
    47161 gacaaaagac tggcggaaca aaccatattg atagttcaga ccgt
//
LOCUS       DAMZET010000014        45582 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.014, whole genome shotgun sequence.
ACCESSION   DAMZET010000014 DAMZET010000000
VERSION     DAMZET010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.014"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(87..794)
                     /locus_tag="POB34_000930"
     CDS             complement(87..794)
                     /locus_tag="POB34_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDI5917922.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1192..3327
                     /gene="speC"
                     /locus_tag="POB34_000931"
     CDS             1192..3327
                     /gene="speC"
                     /locus_tag="POB34_000931"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDI5917923.1"
                     /translation="MKSMNIAASSDLVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTMRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(3377..4633)
                     /gene="nupG"
                     /locus_tag="POB34_000932"
     CDS             complement(3377..4633)
                     /gene="nupG"
                     /locus_tag="POB34_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HDI5917924.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(4835..5914)
                     /gene="mltC"
                     /locus_tag="POB34_000933"
     CDS             complement(4835..5914)
                     /gene="mltC"
                     /locus_tag="POB34_000933"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HDI5917925.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(5979..6254)
                     /locus_tag="POB34_000934"
     CDS             complement(5979..6254)
                     /locus_tag="POB34_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HDI5917926.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6282..7334)
                     /gene="mutY"
                     /locus_tag="POB34_000935"
     CDS             complement(6282..7334)
                     /gene="mutY"
                     /locus_tag="POB34_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDI5917927.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            7495..8214
                     /gene="trmB"
                     /locus_tag="POB34_000936"
     CDS             7495..8214
                     /gene="trmB"
                     /locus_tag="POB34_000936"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDI5917928.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8214..8540
                     /locus_tag="POB34_000937"
     CDS             8214..8540
                     /locus_tag="POB34_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HDI5917929.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            8724..9443
                     /locus_tag="POB34_000938"
     CDS             8724..9443
                     /locus_tag="POB34_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HDI5917930.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            9619..10665
                     /gene="ansB"
                     /locus_tag="POB34_000939"
     CDS             9619..10665
                     /gene="ansB"
                     /locus_tag="POB34_000939"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HDI5917931.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            10782..11789
                     /locus_tag="POB34_000940"
     CDS             10782..11789
                     /locus_tag="POB34_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HDI5917932.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(11944..13080)
                     /gene="hemW"
                     /locus_tag="POB34_000941"
     CDS             complement(11944..13080)
                     /gene="hemW"
                     /locus_tag="POB34_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HDI5917933.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRAEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(13073..13666)
                     /locus_tag="POB34_000942"
     CDS             complement(13073..13666)
                     /locus_tag="POB34_000942"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HDI5917934.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDLKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(13674..13964)
                     /locus_tag="POB34_000943"
     CDS             complement(13674..13964)
                     /locus_tag="POB34_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HDI5917935.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(13961..14527)
                     /gene="yggT"
                     /locus_tag="POB34_000944"
     CDS             complement(13961..14527)
                     /gene="yggT"
                     /locus_tag="POB34_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HDI5917936.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(14545..15249)
                     /gene="yggS"
                     /locus_tag="POB34_000945"
     CDS             complement(14545..15249)
                     /gene="yggS"
                     /locus_tag="POB34_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HDI5917937.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            15267..16247
                     /locus_tag="POB34_000946"
     CDS             15267..16247
                     /locus_tag="POB34_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDI5917938.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGCVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            complement(16410..16826)
                     /gene="ruvX"
                     /locus_tag="POB34_000947"
     CDS             complement(16410..16826)
                     /gene="ruvX"
                     /locus_tag="POB34_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDI5917939.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(16826..17389)
                     /locus_tag="POB34_000948"
     CDS             complement(16826..17389)
                     /locus_tag="POB34_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HDI5917940.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(17498..18448)
                     /gene="gshB"
                     /locus_tag="POB34_000949"
     CDS             complement(17498..18448)
                     /gene="gshB"
                     /locus_tag="POB34_000949"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HDI5917941.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(18461..19192)
                     /gene="rsmE"
                     /locus_tag="POB34_000950"
     CDS             complement(18461..19192)
                     /gene="rsmE"
                     /locus_tag="POB34_000950"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDI5917942.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(19272..19979)
                     /gene="endA"
                     /locus_tag="POB34_000951"
     CDS             complement(19272..19979)
                     /gene="endA"
                     /locus_tag="POB34_000951"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HDI5917943.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(20074..20571)
                     /locus_tag="POB34_000952"
     CDS             complement(20074..20571)
                     /locus_tag="POB34_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HDI5917944.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(20648..22042)
                     /gene="galP"
                     /locus_tag="POB34_000953"
     CDS             complement(20648..22042)
                     /gene="galP"
                     /locus_tag="POB34_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HDI5917945.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(22478..23632)
                     /gene="metK"
                     /locus_tag="POB34_000954"
     CDS             complement(22478..23632)
                     /gene="metK"
                     /locus_tag="POB34_000954"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDI5917946.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            complement(23936..24151)
                     /locus_tag="POB34_000955"
     CDS             complement(23936..24151)
                     /locus_tag="POB34_000955"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917947.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            24287..24418
                     /gene="yqgB"
                     /locus_tag="POB34_000956"
     CDS             24287..24418
                     /gene="yqgB"
                     /locus_tag="POB34_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HDI5917948.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            24427..26403
                     /gene="speA"
                     /locus_tag="POB34_000957"
     CDS             24427..26403
                     /gene="speA"
                     /locus_tag="POB34_000957"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HDI5917949.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            26549..27280
                     /locus_tag="POB34_000958"
     CDS             26549..27280
                     /locus_tag="POB34_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917950.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGMDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            27416..28336
                     /gene="speB"
                     /locus_tag="POB34_000959"
     CDS             27416..28336
                     /gene="speB"
                     /locus_tag="POB34_000959"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDI5917951.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(28542..29300)
                     /gene="loiP"
                     /locus_tag="POB34_000960"
                     /gene_synonym="yggG"
     CDS             complement(28542..29300)
                     /gene="loiP"
                     /locus_tag="POB34_000960"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HDI5917952.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKALSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            29578..31569
                     /gene="tkt"
                     /locus_tag="POB34_000961"
     CDS             29578..31569
                     /gene="tkt"
                     /locus_tag="POB34_000961"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDI5917953.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            31883..32326
                     /gene="cmtB"
                     /locus_tag="POB34_000962"
     CDS             31883..32326
                     /gene="cmtB"
                     /locus_tag="POB34_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HDI5917954.1"
                     /translation="MRLSDYFPESSISVIYSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            32354..33742
                     /gene="cmtA"
                     /locus_tag="POB34_000963"
     CDS             32354..33742
                     /gene="cmtA"
                     /locus_tag="POB34_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDI5917955.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            33757..35034
                     /locus_tag="POB34_000964"
     CDS             33757..35034
                     /locus_tag="POB34_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDI5917956.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            35031..35996
                     /gene="yggF"
                     /locus_tag="POB34_000965"
     CDS             35031..35996
                     /gene="yggF"
                     /locus_tag="POB34_000965"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDI5917957.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            36018..36527
                     /gene="fumE"
                     /locus_tag="POB34_000966"
     CDS             36018..36527
                     /gene="fumE"
                     /locus_tag="POB34_000966"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HDI5917958.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            36524..37237
                     /locus_tag="POB34_000967"
     CDS             36524..37237
                     /locus_tag="POB34_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HDI5917959.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVSPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(37209..37886)
                     /locus_tag="POB34_000968"
     CDS             complement(37209..37886)
                     /locus_tag="POB34_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HDI5917960.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCGQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(37880..38557)
                     /locus_tag="POB34_000969"
     CDS             complement(37880..38557)
                     /locus_tag="POB34_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDI5917961.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(38545..39252)
                     /locus_tag="POB34_000970"
     CDS             complement(38545..39252)
                     /locus_tag="POB34_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDI5917962.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(39253..39831)
                     /locus_tag="POB34_000971"
     CDS             complement(39253..39831)
                     /locus_tag="POB34_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDI5917963.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(39854..40285)
                     /locus_tag="POB34_000972"
     CDS             complement(39854..40285)
                     /locus_tag="POB34_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDI5917964.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQQQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            40657..41676
                     /gene="epd"
                     /locus_tag="POB34_000973"
     CDS             40657..41676
                     /gene="epd"
                     /locus_tag="POB34_000973"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HDI5917965.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            41726..42889
                     /gene="pgk"
                     /locus_tag="POB34_000974"
     CDS             41726..42889
                     /gene="pgk"
                     /locus_tag="POB34_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDI5917966.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            43104..44183
                     /gene="fbaA"
                     /locus_tag="POB34_000975"
     CDS             43104..44183
                     /gene="fbaA"
                     /locus_tag="POB34_000975"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HDI5917967.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            44541..45401
                     /gene="mscS"
                     /locus_tag="POB34_000976"
     CDS             44541..45401
                     /gene="mscS"
                     /locus_tag="POB34_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HDI5917968.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
BASE COUNT        11099 a        11394 c        12110 g        10979 t
ORIGIN      
        1 gattcgcctc gtctcgtcaa tcaaatttct tcaatttact gcagactgca caatctatca
       61 tcactttgcc gctattgcac gcattgtcag gcaaaccagg cggtccaggc ggcggaaagc
      121 ggcattgcca gaaggagtgc tggaagcatg ttgaccaccg ggaacatttt aatgccacag
      181 atgcgtaatc cggttgccag cagcaataaa ccgcctacgg cgctgaagtc tgccatcatc
      241 gacggtgtgg tcagcggtaa tatcagcgcg gcagcccacg ccagcgttaa ctggatgatc
      301 agtaatggga tactaattac cgacaccgca atacccagcg agcaggcgaa gatcatcgcc
      361 gtaaagaaat caagaaatga cttggcgatt aaaatactcg gatcgccggt catcccttcg
      421 ttcatcgccc cgaagatccc ggtgccgctg gcgcaaaaca ggacaataat cgcgacataa
      481 ttctgaataa aagattcatg cgctggcttc ttacgtgagt gacgaaacag attttgtgct
      541 ttggtgactg ctgtattgac acctttctcc agcaaacaaa tttcgccaat tagagcgcca
      601 agtagcgtag ctaaaaccat cgctggaagg ttggcacatt tcaccaccag taaaatacca
      661 atccccagcg atgccagacc aaaaatagat gtcatggaga cgcggatacg ttccggtaag
      721 cgttggctga gcagtgcgcc gagaacgcca cccagtaata ctgcactagc gttgataaaa
      781 gggccgatga ccacaagagt tcctgttaac tgtattgttt gattttctta ttatgaccgt
      841 ttggtgccgt cgatggcttg tttgttcgca aagtcctggc ttgcacgctt tagcgaaagg
      901 tgccatgatt gcgcgaattt tctcctctct gtacggagtt tgcccgatgc acgccacctc
      961 cttacattct ctcgcttatc gccgtttcgc gcgaaacgtt tccctttcta tgttactgct
     1021 catgcggtga agttaacgca cgctcactgc aggacaacag taaaatcaga gcgtttctgc
     1081 ttttactgat gtctggcggt cggagctggt gaccagtttg acccatatct catggggcag
     1141 ggttttccac cttgtccggt attcttactt ccccgaaacg ggtttgcgct tatgaaatca
     1201 atgaatattg ccgccagtag tgatctggta tcccgacttt cttctcatcg tcgcgtggtg
     1261 gcgttgggag atactgattt tacggacgtc gcggcagtcg tcattaccgc tgcggatagt
     1321 cgcagtggca ttcttgcgtt gcttaagcgc accggttttc atctaccggt gtttttgtat
     1381 tccgaacatg ctgttgaatt acctgcgggc gttacggcgg taatcaacgg caacgagcag
     1441 caatggctgg agctggaatc cgcagcctgt cagtatgaag agaatttgct gccaccgttt
     1501 tatgacacgc tgacgcagta cgttgagatg ggcaacagca cctttgcttg ccctggacat
     1561 caacatggtg cgttttttaa aaagcatcct gccggacgcc atttttacga tttctttggt
     1621 gagaacgtct ttcgcgccga tatgtgtaac gctgacgtaa aattgggcga tctgcttatt
     1681 catgaaggat cggcgaaaga tgcgcagaaa ttcgcagcca aagtctttca tgccgataaa
     1741 acctattttg tgctgaacgg cacatcggca gcgaataaag tggtgacgaa tgcgctgtta
     1801 acgcgtggcg atctggtgct cttcgaccgt aacaaccata agtcgaatca tcacggcgcg
     1861 ctgattcagg cgggggcgac gccggtctat ctggaagctt cacgcaaccc gtttggtttc
     1921 attggcggta ttgatgcgca ctgttttaat gaagagtatc tgcgccagca aattcgcgac
     1981 gttgcgccag aaaaagctga gttgccacgc ccgtttcgcc tggcgattat tcagctggga
     2041 acctatgacg gcactgtcta taacgcccgt caggtgatcg ataccgttgg gcatctgtgt
     2101 gattacattc tgtttgattc cgcgtgggtc ggttatgaac aatttatccc gatgatggcg
     2161 gatagctcgc cgctgctgtt agaacttaac gaaaacgatc cggggatctt tgtgactcag
     2221 tcggtgcaca aacagcaggc gggattctca cagacgtcgc agatccataa aaaagataac
     2281 catatccgcg gacaggcgcg tttttgcccg cataagcggt tgaataatgc ctttatgctc
     2341 catgcttcta ccagcccgtt ctacccgctg tttgccgcgc tggacgttaa cgctaaaatt
     2401 catgaaggag agagtgggcg tcggctgtgg gctgagtgcg ttgagttggg tattgagtcg
     2461 cgcaaggcta ttcttgcgcg ctgtaagctg ttccgcccgt ttatcccgcc cgttgttgat
     2521 ggcaaattgt ggcaggatta tccgacatct gtgttagcca gcgaccgccg ttttttcagt
     2581 tttgagccgg gggcgaagtg gcacggcttt gaaggatatg ccgcggatca gtattttgtt
     2641 gatccgtgca agctgttact cactacacca ggtatcaatg ccgaaactgg cgaatatagc
     2701 gactttggcg ttccggcgac gattctggcg cactatctgc gtgagaacgg cattgtgccg
     2761 gagaagtgcg atctcaactc cattctgttc ttattaactc cggcggaaag ccacgagaag
     2821 ctggcacaac tggtggcgat gctggcgcaa tttgaacagc atattgagga tgactcgccg
     2881 ctggctgagg tgttgccgag cgtttataac aaatatccgg tgcgctatcg cgactacacc
     2941 atgcgccagt tgtgtcagga gatgcacgat ctgtatgtca gtttcgacgt caaagaccta
     3001 caaaaagcga tgttccgcca gcagagtttc ccgtcagtgg tgatgaaccc ccaggatgcg
     3061 catagcgctt atattcgcgg tgaagtggag ttggtgcgga ttcgtgatgc cgaagggcga
     3121 attgcggcag aaggggcgtt gccttatcca cctggcgtgc tttgcgtggt acccggggaa
     3181 gtctggggtg gggcggttca acgttatttc cttgcactgg aagaaggggt gaatttgttg
     3241 ccgggatttt cgccggagct gcaaggtgtt tatagcgaaa ccgatgcgga tggcatgaaa
     3301 cggttgtacg gttatgtgtt gaagtaagaa taaaaaaaac gggtcacctt ctggcgaccc
     3361 gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc tgtcgggaca cgaacgtgtt
     3421 tatatttgaa catcgccatg aacgcgaagg ccagaaccac ggagtaacca gcgaaaatca
     3481 accatacggt ctgccagtcg gtaatgccgt tttgggtgta catctcaaca actttaccgc
     3541 tcacgatgcc gccgaggata cagccgaagc cgttagtcat catcaggaac atcccttgtg
     3601 cactggcgcg aattgccggg ctaacttctt tttcgacaaa caccgaacca gagatgttga
     3661 agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac cagcagtaca gtaccgaacg
     3721 gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat ccacgccaca atactgatca
     3781 tcattacgtt cttaataccg tagcggctta agaagaacgg gatggtcaga atgaacaggg
     3841 tttcagagat ctgcgaaatc gacatgatga ttgacgcatg ctgcacaata aagctgctgg
     3901 caaacatcgg atctttgtcg aagctgtgca ggaaggtatt accgaacatg ttggtaatct
     3961 gcagttccgc gcccagcagc attgagaaga taaagaagat tgccatacgc ttgtttttaa
     4021 acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct ctgattcgct tgctgtttag
     4081 caaccggaat atgcggcaga gtcagggtaa acagaaccag aatggcggaa agtgctgcgc
     4141 caatatacag ctgcatgtgg cttaattcga agccagacag gctcaccacc cacattgcca
     4201 tgataaagcc gatggtgccc cagatacgga ttggcgggaa gtcagtaacg atatccatcc
     4261 cggcattttg caggcgatag taagagatgg tgttgattaa cccaagcgtt ggcatataag
     4321 caaacgagtt aatcaatatc acaaggaaca tcgcctccgg tgtcgtgacc tgtgccgcca
     4381 tgaacagcgt gatagcgcca atggtgtggc aaatggcata tacccatttc gcacttaacc
     4441 atttgtcggc cacaatcccc agcagcgcag gcataaatac cgcggcgata cccagtgagc
     4501 tataaactgc gccaatagaa gcaccgtcaa acttcagggt aacaaacata taggagccga
     4561 gggtcgtcag ccaacttccc cacagacaga actgcagaaa agagaggatt ttcagctgca
     4621 gcttaagatt catgttaatt tcctcacatc gtgatgcgga tgagtgtttg aaaaagcaca
     4681 tttgaccgtg cgagatgatg ggattctatc aacttcgcgg actggttttt tgttacctgt
     4741 ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat tgtgatgtgg ataacatttt
     4801 tgcccctgag catcgtcagg ggcggttaat ggaattatcg gcggcggtag gatttttgcg
     4861 cggtattcac tttataaaga taacggcgag attccgcaga gggatggcgg gtcgtcagcg
     4921 tctgataaac atcgcccggc gtcatggtgt taataatatt ggcagcctga atcttatcat
     4981 tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata ggcggtgatg acggcataac
     5041 gccgcgatgt tgggttatca attccgccga gataaacatt gttcagcatc gccagatacg
     5101 cggtgccggt atcaatattg ctggcaggat caaacaagaa actgcggctc ggcgtgccgg
     5161 atttcccctg cgagcggaac acatctttcc cggcagtatg ttgtaccacc tgcattaatc
     5221 ccagcgcatc ggaacggctg accgcatatg ggttaaagga agattcggtc tgcataatcg
     5281 ccagaatcag cgactcatca acgccatatt tccgtgacgc ctggcggacc atgccgagat
     5341 atttgtgcgc acgtttatca aggtggttcg gcaccatgtt aatggtaacg ctgtagatga
     5401 tacgcagccc gttgctgcgg ctcttcagac ggtttttcag cagataatcc gcgaagttgc
     5461 tcgcgcgacc ttcccagcga atcggctgcc cggtgttgtc caccacctga ccgtaaagga
     5521 aaggttcttt cgaaatcgta atatcatcaa cgtcggaata gagatcgacc gaactcggat
     5581 cgtcacccat cagcaacgtt ttgataattg cccggcgcaa atgcgcggca ggttctgtcc
     5641 cggcgatagt ttcgatagta atcgtaccgt catcgaagtt gatgtggctg cgggtctgat
     5701 attgatcggt gtatttcacg tagtccttag gaccggcgat caccacctct ttgaatcccc
     5761 agatgttctc aatattgtgg gcaaattgcc ccatcagaat atcaaaaccg ttggtatctt
     5821 tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt cgaacaggag atgagcaacg
     5881 gcgcaatcaa agccagcgcg agatattttt tcatcattcc gggtgcgtgt tgtgtttgtt
     5941 tttatgcaag ttgccggagg cgtgctccgg cactgttttt attttttatc ttccggcgta
     6001 tagccctcga tatgcacctc tttaccctcg aacaggaagt tgaccatctc ctgctccagc
     6061 agcttgcggt gctcggcatt catcatgttg agtttctttt cattaatcag catggtttgt
     6121 ttgtgctgcc actgcgccca ggcttctttg gagatctcgt tatagatgcg ttttcccagc
     6181 tcgccggggt acagctgaaa atcctgacct tctgcttcac gttgcaggaa agtacaaaaa
     6241 atcgttctgc tcataaatca tcctctttat cgactcacgc gctaaaccgg cgcgccagtg
     6301 cgtaactgct gtaacaaacg ctccacggga gccgccaggc caactgacgg cggttgcgct
     6361 aagttatacc agagcgcatt gccttcatcc atgcagccgg tgaatgacga cacgggaagc
     6421 cacataggca caatatctaa gtggaaatgg ctgaaggtat gccgaaacgc ggtcagctgc
     6481 gtcaggttat cggcagcaat ctgccgttgc gccagccact gccgcaaact ttcttcgtcg
     6541 gcaaactgcg ggaaacagta taaaccgccc cacaatccgc tcggcggacg ctgcgccagc
     6601 aatacttcat cttcgtgctg taacaggaga aagtagccgg tgcgctccgg cagcgtctgt
     6661 ttcggttttt tgcccggata aagcgaccag ctattgttgg cggcggcaat acatccgttt
     6721 tgtagcggac agagcgaaca tttcggtttc gagcgcgtac aaatcatcgc acccaaatcc
     6781 atcatcgcct gattaaaccg ttccacgcca accgcgggcg tcacctgctc gctcaaactc
     6841 cataatttat tctcgacctc ttttttccct ggccagccgc ttacagcata gcagcgcgcc
     6901 agcacccgtt tgacgttacc gtcgagaatc ggaaagtgct tacccagaga aagcgagaga
     6961 atcgcacctg cggtggaacg cccgacgccc ggtaacgccg cgacttcttc aaaggtttcc
     7021 gggaatttac cgccgtgtaa ggtcgccact tgttgtgccg ctttatgcag gttgcgcgcg
     7081 cgggcgtaat agccaagccc ggtccacaag tggagaactt cgtcgagcgg cgcattggcg
     7141 agatcggtca ccgtcgggaa gcgcgccata aagcgttcaa aataggggat aacggtcgca
     7201 acctgagttt gttgcaacat cacttctgag agccatactt tgtagggcgt cttgtcaatt
     7261 tgccagggca gcgttttccg cccgtattta tcgtaccagt ccagaacctg ggctgaaaat
     7321 tgcgacgctt gcatggtcac cgaattcact gttgttgggg gcaagattgc agcacagcga
     7381 cggcagggtg taaaccggaa ctttccgcag ccacggcctt atcatgcttg catccggcaa
     7441 ctaactttgg ataatgcccg ttttcagaac actttcacaa gcgactaaat ctttatgaaa
     7501 aacgacgtca tttcaccgga atttgatgaa aacggccgcc cactgcgccg tatccgtagt
     7561 tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac atgcgctgga aaactactgg
     7621 ccggtgatgg gcgttgagtt cagcgaagat atgctggatt tccccgcgct ttttggccgt
     7681 gaagcgccgg tgacgcttga gattggtttt ggcatggggg catcgctggt ggcaatggct
     7741 aaagatcgcc ccgagcagga cttcctcggc attgaagtgc attcaccggg cgttggtgcg
     7801 tgcctggctt ctgcgcatga agaaggttta agcaacctgc gagtgatgtg tcacgatgcg
     7861 gttgaagtgc tgcataaaat gattcctgac aattcattgc gcatggtgca gctctttttc
     7921 cctgacccgt ggcacaaagc gcgccataat aaacgccgta tcgtacaggt gccgtttgcc
     7981 gaactggtaa aaagcaaact gcagctgggg ggggtattcc atatggcgac cgactgggaa
     8041 ccttatgcgg aacatatgct tgaagtgatg tcttctattg acggttataa aaacctgtca
     8101 gagagcaatg attacgtacc gcgtccggca tcacgtccgg tgacgaaatt tgaacaacgt
     8161 ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg agagggtgaa ataatggcaa
     8221 agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga cgaattccag gaattaggat
     8281 tttcggtggc atggcgattc ccggaaggta catcggaaga acagattgat aaaaccgttg
     8341 atgattttat taacgaggtt atcgaaccga acaaactggc ctttgacggc agcggttatc
     8401 tggcctggga aggtctgatc tgcatgcagg aaatcggcaa atgcaccgaa gaacatcagg
     8461 cgattgtgcg taagtggctg gaagagcgca aactggatga ggtacgcacc agcgaacttt
     8521 tcgacgtttg gtgggactaa gaaagcatac gggcgatgac aaatgcaaaa ctgcctgatg
     8581 cgctacgctt atcaggcctg gaaagatgca cgatcgagta ggcgggataa ggtgtttacg
     8641 ccgcatccgg catggaaaac gcgtactttg ttatcaatct ggggccagca aatgctggcc
     8701 tgattttttc ttgagggaag actatgatgc gcaaaatgct gctggcggca gcactttcag
     8761 tgacggcaat gaccgctcac gccgactacc agtgcagcgt cacgccgcgt gacgatgtga
     8821 ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg caatctggtg atcacgccag
     8881 acggcaacgt gatgtataac ggtaagcaat attccctgaa tgccgcccag cgcgagcagg
     8941 cgaaggatta tcaggctgaa ctacgcagca cgctgccgtg gattgatgaa ggcgcgaaaa
     9001 gccgcgtcga aaaagcccgt attgcgctgg ataaaattat cgttcaggag atgggcgaaa
     9061 gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca gctgaaagag cagatgaacc
     9121 gcattatcga aacgcgcagc gatggcctga cgtttcacta taaagccatt gatcaggttc
     9181 gcgccgaagg ccagcaatta gtgaatcagg caatgggtgg aattttacag gacagcatta
     9241 atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa cccattacag aatgtgctgg
     9301 gaagcctggg ggggctgcaa tcctcaatcc aaaccgagtg gaaaaagcag gaaaaagatt
     9361 tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac tctggaagat agccgcaaag
     9421 ccctggtcgg gaatttaaaa taatcctcta ttttaagacg gcataatact tttttatgcc
     9481 gtttaattct tcgttttgtt acctgcctct aactttgtag atctccaaaa tatattcacg
     9541 ttgtaaattg tttaacgtca aatttcccat acagagctaa gggataatgc gtagcgttca
     9601 cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg cacttgccgc actggttatg
     9661 ggttttagtg gtgcagcatt ggcattaccc aatatcacca ttttagcaac cggcgggacc
     9721 attgccggtg gtggtgactc cgcaaccaaa tctaactaca cagcgggtaa agttggcgta
     9781 gaaaatctgg ttaatgcggt gccgcaactg aaggacattg cgaacgttaa aggcgagcag
     9841 gtagtgaata tcggctccca ggacatgaac gataatgtct ggctgacact ggcgaaaaaa
     9901 attaacaccg actgcgataa gaccgacggc ttcgtcatta cccacggtac cgacacgatg
     9961 gaagaaactg cttacttcct cgacctgacg gtgaaatgcg acaaaccggt ggtgatggtc
    10021 ggcgcaatgc gtccgtccac gtctatgagc gcagacggtc cattcaacct gtataacgcg
    10081 gtagtgaccg cagctgataa agcctccgcc aaccgtggcg tgctggtagt gatgaatgac
    10141 accgtgcttg atggccgtga cgtcaccaaa accaacacca ccgacgtagc gaccttcaag
    10201 tctgttaact acggtcctct gggttacatt cacaacggta agattgacta ccagcgtacc
    10261 ccggcacgta agcataccag cgacacgcca ttcgatgtct ctaagctgaa tgaactgccg
    10321 aaagtcggca ttgtttataa ctacgctaac gcatccgatc ttccggctaa agcactggta
    10381 gatgcgggct atgatggcat cgttagcgct ggtgtgggta acggcaacct gtataaatct
    10441 gtgttcgaca cgctggcgac cgccgcgaaa aacggtactg cagtcgtgcg ttcttcccgc
    10501 gtaccgacgg gcgctaccac tcaggatgcc gaagtggatg atgcgaaata cggcttcgtc
    10561 gcctctggca cgctgaaccc gcaaaaagcg cgcgttctgc tgcaactggc tctgacgcaa
    10621 accaaagatc cgcagcagat ccagcagatc ttcaatcagt actaatcacc tcgccccggt
    10681 atcgtgccgg ggctttttca cttcagactt acgtccattg ccaattttca ttaccctaat
    10741 gataatcacc gtaataaatt attccgcgtg aggtttttcg ggtgaaaaag caatggattg
    10801 ttggcacggc actgcttatg ttgatgactg gtaatgcctg ggcagatggc gaaccgccaa
    10861 ctgaaaatat cttaaaagat caattcaaaa agcagtatca cggcattctc aagcttgatg
    10921 ctatcacctt aaaaaatctt gatgctaagg gtaatcaggc cacctggtca gcggaaggcg
    10981 atgtctcttc cagtgacgat ctctatacct gggtcggtca gttggcagat tacgaactgc
    11041 tcgaacagac ctggacgaaa gataaaccgg taaaattctc ggcgatgtta accagtaaag
    11101 gaacgcccgc gtctggctgg tcggtgaact tttactcttt tcaggcggca gccagcgatc
    11161 gtgggcgggt ggtcgacgat atcaaaacga ataataaata tctgatcgtg aatagcgaag
    11221 atttcaatta tcgctttagt cagcttgaat ctgcgttgaa tacccagaag aactcgattc
    11281 ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat ggtggcggcg cagaaagcag
    11341 cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat gacccgcgaa gaagccttta
    11401 aaaaaattca ccaacagcgt gatgagttta ataaacagaa cgatagcgag gcgttcgcgg
    11461 ttaaatatga caaagaggtt tatcaaccgg cgattgcggc atgtcataaa cagagtgaag
    11521 agtgttatga agtgccgatt cagcagaaac gagatttcga tatcaacgaa caacggcgac
    11581 agacttttct gcaatcacaa aaactcagcc gtaaattgca ggatgactgg gtaacgctcg
    11641 aaaaagggca atatccgtta acgatgaaag tttcagaaat aaacagtaaa aaagtcgcga
    11701 ttttgatgaa aattgacgat atcaaccaag ctaatgaacg ctggaaaaaa gataccgaac
    11761 aactgcggcg caatggggtg attaagtaag tttgatatag tgcacgattt gtagcaggat
    11821 gcggcgttga ccccgcatcc ggcaatcaac gtctgatgcg acgcttgcgc gtcttatcag
    11881 acctacaacc gctgccacat gtcggctgga taaggcgttc acgccgcatc cggcaataca
    11941 agtttactca gccagaaaaa gctccagcag cgaatttaaa aacagcttcc catgttccgt
    12001 tatctgccag taatccgcac attcggtgag ataaccctgg gcaatcgcct cgtctaactg
    12061 tgggcgaatc acatcttcgc aaagcccggt atacgcacta aactctgcgc gtggcgcggc
    12121 ctccagcaag cgaaagcggt tcataaagaa ctcaaacggc ttatctgcgg cttcgacatc
    12181 acgctggctt tccagatacc ttccttgcat aaaaccacgc ggatgacgcg ttttagtggt
    12241 acgcagaatg cgcccatccg ggaaggtcac tttgccgtgc gcgccgcagc caataccgat
    12301 gtagtcgcca aagcgccagt agttgagatt gtgctggcac tgataactgg gtttggcgta
    12361 agcggaagtt tcatattgct gataacccgc tgcggttaat aactgatgcc cctgttcgaa
    12421 tatatcccac agcgcgtcgt cgtccggcag taccggcggg cgtgaaccaa acagcgtatt
    12481 aggttcgatg gtcagttgat accaggaaag atgcggcgga ttcagttcaa tggcctggcg
    12541 tagatcgcca agcgcctctt ccagtgattg atccggtagt ccatgcatca aatcgaggtt
    12601 aaagctacgc agccccagcc cgctcgccag ctttgccgcg cgtttcgctt cttgcgggcc
    12661 atgaatacgc ccaagtcgtt tcagcttttc ttcgctaaaa ctctgcacac caatagagat
    12721 gcggttcaca ccagcacgct gataatcgac aaagcgatcg gcttctaccg taccagggtt
    12781 cgcttccata gtaatttctg catccgctgc cagcggcaaa cgcgcacgca cgccgtccag
    12841 cagcgtttgc atcgccgggc cggaaagcag gctcggcgta ccaccgccaa taaagattgt
    12901 ctttacttca cggccctgag cgtaagccac atcattgtcc agatcgcaaa gcagatgctg
    12961 aacgtaatcg tcgtgcggca cttctccttt caacgcgtga gagttgaaat cgcagtacgg
    13021 gcatttctgc acgcaccacg ggatgtgaat gtagagactc agcggcggta atttaaccat
    13081 tacgtaaagc gtccaacagc agtttcaatg cctgaccacg gtgggaaatg gcgctctttt
    13141 cttcgcgggt cagttcggca gcggttttcc cttcggaagg tacgaagaag attggatcat
    13201 aaccaaagcc accagtgccc gctggttcac gagtaatcac gcccggccag ctaccgtggc
    13261 acaccagcgg agtcggatct tccgcgtgac gcagatatac cagcacgcag tggaaacgcg
    13321 cctgacgttg gtcgtccggt acgtctttca gtgtttccag tagtttttgc agattcttta
    13381 gatcggtcgc gtcttcaccg gaataacgcg cggagtaaat gcccggcgcg ccgccaagca
    13441 catctaccgc cagaccagag tcgtcggcaa ttgccggtaa accggtcact tttgccgcat
    13501 ggcgcgcttt cagaatcgcg ttttcgataa aggtcaggcc ggtttcttca gcggaatcta
    13561 cgccgaggtc tgtttgggcc acgatatcaa gaccgaagtc gctaagcagc gacgccagct
    13621 cacgcacttt accggcattg ccggttgcga ggacaacttt ttgcatagga tacctaatta
    13681 attaacgccg cgatttctgg cgggatttgt tgcggattaa tgattttaat ttgtttgtgg
    13741 cggccaagtt cgcctttttc aatcaccacc tggcttttgg caacccggaa ttgcttaccg
    13801 agaaacttca ccagatgact gttggcctgg ccgtcaaccg gcggcgcggt aatggcgact
    13861 ttaacttcgt cgccatgtaa accgacaata gaatcacggc tggctttcgg ctgaatatag
    13921 agccgtaaaa ccagaccgtc atcattaact gttacggcac tcataacgcc atccacagcc
    13981 ccggcagcag catgtttccg gttgcctgta atacttctgc gacacccata ttgatgacat
    14041 acagcagcag aacgaggatc atcggcgaga aatcaatccc acccattgcc ggtagcaggc
    14101 ggcgaatcgg gcgcagcagc ggatcggcca gctgaatcag cacgtattca atcggactac
    14161 gaccctggct tacccagctc ataatcgcca tcaccagcag gacccagaaa atcagcaagc
    14221 cgatggtttt cagcagaatc agtaaaccgg caatccagat gattggcagg aaggtcacca
    14281 ctttaaacag cacaatggct ttgataaaac tgagaatata ggcaaccagc agcgaggcgc
    14341 tgtcaatggg acccattgcc ggaataacgc ggcgcagtgg cccgataatt ggctgcgtta
    14401 ccttcactac aaactgtgag aaggggttgt aaaaatcaca atgagcccac tgcatccaga
    14461 tgcgtaataa cagcaccatg gtatacagct caatgaccgt tgaaagcagg aaagtcaacg
    14521 tattcatggc gttcctttaa ttccttattt tttagagtaa tcacgcgcac caaaaattgc
    14581 agtgccgata cgaaccatcg tgctacctgc cgcaatggcg gcttccatat cgtccgacat
    14641 tcccagagag agcgtgtcga tatgcgggta gcgcgttttc agtccggcaa atgctacagc
    14701 catttggcgt gcaacttcaa actgccttac atattctgac tcaggcgcag ggattgccat
    14761 cagcccgcgc agacgtaaac gcggtagttc agcgaccgcc gctgccagct cgtccagttc
    14821 tgccagttga atcccggact tactgttttc atcactaata ttaatttgaa tcagtacgtt
    14881 aagaggggga agttctgccg ggcgctgatc gttgaggcgg gtagcaatgc gcaaacggtc
    14941 gatggtatga caccagtcga aatgctctgc caccaggcgg cttttattag actgcaacgg
    15001 gccaataaaa tgccattcta atcctgttac gcccagttcc tgaaagtggc gaattttatc
    15061 taccccttcc tgaacgtagt tttcaccaaa ttgacgctgc ccggcatcaa tggcttctgc
    15121 gatggcgctc gcaggttttg ttttactgac tgcaagcagc gtaatttctt ctggagaacg
    15181 gccgcaacgc gttgcagccg ctgagatttt gtcccggacc tgtgccaggt tatgcgcaat
    15241 atcgttcatt ttccgaggat cttagtatga atatggaaga aattgtggcc cttagtgtaa
    15301 agcataacgt ctcggatcta cacctgtgca gcgcctggcc cgcacgatgg cgcattcgtg
    15361 gcagaatgga agctgcgccg tttgatgcgc cggacgtcga agagctactg cgggagtggc
    15421 tggatgacga tcagcgggca atattgctgg aaaatggcca gctggatttt gccgtgtctc
    15481 tggcggaaaa ccagcggttg cgtggcagtg cgttcgcgca acggcaaggt atttctctgg
    15541 cattacggtt gttaccttcg cactgtccac agctcgaaca gcttggtgcg ccaccggtat
    15601 tgccggaatt actcaagagc gagaatggcc tgattctggt gacgggggcg acggggagcg
    15661 gcaaatctac cacgctggcg gcgatggttg gctatcttaa tcaacatgcc gatgcgcata
    15721 ttctgacgct ggaagatcct gtggaatatc tctatgccag ccagcgatgt ttgatccagc
    15781 agcgggaaat tggtttgcac tgtatgactt tcgcatcggg gttgcgggct gcattgcggg
    15841 aagatcctga tgtgattttg ctcggagagc tacgtgacag tgagacaatc cgtctggcgc
    15901 taacggcggc agaaaccggg catctggtgc tggcaacctt acatacgcgt ggtgccgcgc
    15961 aggcagttga gcgactggtg gattcatttc cggcgcagga aaaagatccc gtacgtaatc
    16021 aactggcagg tagtttacgg gccgtgttgt cacaaaagct ggaagtggat aaacaggaag
    16081 gatgcgtggc gctgtttgaa ttattgatta acactcccgc ggtggggaat ttgattcgcg
    16141 aagggaaaac ccaccagtta ccgcatgtta ttcaaaccgg gcagcaggtg gggatgataa
    16201 cgtttcagca gagttttcag cagcgagtgg gggaagggcg tttgtgacgt tgccatgccc
    16261 gatgtgcaag cttatcgggc ctacaaaggc aataaacgtc tgatgcgacg cttgcgcgtc
    16321 ttatcagacc tacaaccgct gccacatgta ggccggataa ggcgtaaggc gcgatgatat
    16381 tatacactcc agcaggcgtt taaatcgctt taatatccct gctcgaaata gctttcgaga
    16441 ataataaccg cagaggcaga gtcgactttg cctttattta gcgcccgata gccgccctgt
    16501 tcaaacagac cggaacgggc ttccacggtg ctaagacgtt cgtcatggag ctttacttca
    16561 acaccgaaac gaccatgaat acggttggca aatttacgcg ctctggcagt caatggttgc
    16621 tcggtgccgt ccatattcag cggcaaaccg acgatgattt cgtccggctg ccactctttc
    16681 agtaaacgct cgataatgtt ccagtccggc gtaccgtcct gtgctttaat tgcaggcaaa
    16741 gggcgagcgg tgccggtaat gcgttggccg accgctacac caatgctttt ggtaccgaag
    16801 tcgaaggcga gtaaggttcc actcatcagg cgtgccctgc cacaccaggc atggtgagaa
    16861 tatccacacc aatcagtttt gccgcctcgc gccagcggtc ggcaatcggt gttttgaaca
    16921 gaatattcag atccgccggg gccgttagcc acgcgttatc gagaatttct tgttccagtt
    16981 gacctttttc ccaggaggca taacccagag ccaccaatac gtcagacggt tgtttatcgg
    17041 tgccgagcgt ttccagcaca tcgcgggaag tggtcatcac tgtgttgtct gaaatgcgaa
    17101 tgctggaagc aaaattggag ggcggagtat gcaaaataaa cccgcgatct tcagccagcg
    17161 gaccgccgag cataaccggt ttatccagac ggattgattc atcacgcggc tccggcgtga
    17221 tcttcagctt ttccagaatc ccttcaattt tgagattttc cagcggcttg ttgacgatga
    17281 tccccattgc accattggta ttatgttcgc aaatgtagac cacggaacga cggaaaatcg
    17341 gatcctggag agcaggcatg gcaataagaa agtgatgctg taaattcatt gtcagaggtt
    17401 ctgtttcctg gttcaaaaaa gcaacagcgc ccagtatgcg tggaaagaag ggcgctgtca
    17461 ctcagagtct caacgagatc cttctcgcta aggtgggtta ctgctgctgt aaacgtgctt
    17521 cgatggcatc cattaacatt ccggtgatcg acaccggaaa ctctgcttca atctcacgaa
    17581 tacaggttgg gctggtgacg ttaatttcag tcagacggtc gccgatgata tccagaccaa
    17641 caaaaatcag ccctttttct ttcagcgtcg gcccgatctg acgggcgatt ttccagtcac
    17701 tttccgtcag cggacgaggt tcaccgcgtc caccggcagc cagattgcca cgggtttcgc
    17761 ccccctgcgg aatacgcgcc aggcagtacg gtaccggctc gccatccacc accagcacgc
    17821 gtttgtcgcc atctttaatg gctggcaggt aattttgcgc catgcagtag cgagtgccat
    17881 gctcagtcag ggtttcggca atcacgccga ggtttggatc gccttctttc acgcggaaaa
    17941 tcgacgcgcc gcccataccg tccagcggct taagaatgat gtcgctgtgt ttctcccaga
    18001 acgcttttag ctgcgcttta ttgcgcgtaa ccagcgtttc tggcgttaag tcagagaacc
    18061 aggcggtaaa cagtttctcg ttacagtcgc gcaggctctg cggcttgtta acgatcagcg
    18121 tccctttctc ttcggcacgt tccagaatat aggtcgcgta gataaactcg gtatcaaacg
    18181 gcgggtcttt acgcatcagg atcacatcga gatcggccag cggcagatcc tgttcaccga
    18241 cgaatgaaaa ccactcttcg tagttctgct tcacgttcag cgtgcgggta tgggcgcggg
    18301 cttcgccatt gatcagatac agatcgccca tctccatata gtgaagttcg taaccacgac
    18361 gctgtgcttc cagcaacata gcgaaactgg aatctttctt gatgttgatg tttgcgatgg
    18421 ggtccatcac gatgccgagc ttgatcatta ttcttctccg ttagcccaaa tcgccaaatc
    18481 gtacttgtag cgcggtaatg gcggtgagcg cagttgtctc tgtacgcaaa acgcgaggtc
    18541 ccaacaggat atcagtaaat tgatagcggg cagtcatggc aatttcatct gccgataaac
    18601 cgccttccgg gccaatcagc aggcggacgc gttcaaccgg taacggcaac gtattgatgc
    18661 tgttactggc gcgcgggtga agattcagtt tcagtccttc atcctgctct gcacaccagg
    18721 cttccagatc catcgctggg cggatttccg gcacccggtt acgaccacac tgctcacagg
    18781 cagcaattgc aatcttctgc cactgctgga gcttcttgtt cagacgttca ctatccagtt
    18841 taacgccgca gcgctcagaa aaaagtggcg taatgaggct tacaccgagt tcgatcgatt
    18901 tctggatcgt aaattccatt ttttcaccac gcgacatcac ctgaccgagg tgaatatgca
    18961 gcggagattc gcgatcgtcg atcttgcctt ccagcacctt cacttccacg ctttttttgc
    19021 tggcgctggt aatttcggcg tcaaagacct ggttgctacc gtcaaacaat tgcaacgcct
    19081 gccccggccc catgcgcagt acgcgcccga tatggttggc ggcatcttcg caaagcgcga
    19141 tgtgagaatg gctggtcagt ggttcaggat gataaatgcg ggggatacgc atagttaaaa
    19201 atccgcgtcg tctccccacg cggttgtacg cgtggggtag gggttaacaa aaagagtccc
    19261 gctagtgtag gttagctctt tcgcgcctgg caagcgcgtt gcacatacgg gttatgattg
    19321 ccttgcacct tcgcgatgcg ttcatcgcgc tcgcactccc agtcggtaac cggatacatc
    19381 ttgttccatg cgttgaacag ctgcgtttgc tggcgagaga gtgtcaggtt gtattggtcg
    19441 cgcatataga agtaggtgcg cgcaatggca ccgcgtgcac gcgctggtgg ttcggcagct
    19501 ttttctttga aatcgacctt catggcgcat tgaccgtact ggccttcgcc gccattccac
    19561 tggctgtaca taaagttgcc gcgatcgcca ttcacctcac cgactgacgg ctgcaggtta
    19621 tgcatatcgc tttccatctt gcgatagacc ggatctttag cgcagttttt acgtccaccg
    19681 tcctgccagc actggcgctg gtgaccgaac tgccaggcgg gaacgacgtg ttcccactct
    19741 acgcggctgg ctcggttttc atttttgcgc acctgatagc cgcacgattg cagatcaaca
    19801 acgccttttt tgccctgcca gttaatttta catccgcaat aaaacgtacc gggcgcgtca
    19861 gcgtggactt ttaccgccgc ggctttcgcc tgagaaaaac tattgatacc ttcggccaac
    19921 gccgggccgg aaaatgctgc gctcagtacc accgcagcaa tagacaaata acggtacatc
    19981 acttactccg tgttaaaacg agccagcaac gtagcgaaag ttgttttggt atgcaatcag
    20041 ccagatcagg aaagttcctg atagttcaga tggttatttc gcaaccagct gttcaccgca
    20101 gtgaacacag cgatagacgg cctcgccacg aactacgcga ttatggcggc gtacggtaag
    20161 ctgatgctcc tggcacttgc agcggtaggg gaaggtgttg cgacgcacgg attgcagttc
    20221 gaactgatgc gtacgacggg cgggaacgcc cagcacgctt tccatcatcc acttccactc
    20281 tttgccatgt ggcgctacgc ggccgaaatg tttccatacc agcaaatgtg ccagttcgtg
    20341 cggtaccact tcttcaataa aagcttcact gttttccaac agcaaaacgg gattgaggcg
    20401 gatttcatag ctttccagcc aggctgttcc ggcggaggtt ccgcgctggg tgtaagagag
    20461 ttttggctcc gggtagttac gccctagctt caggttggcc tgggcgagtt tttcccgcag
    20521 gcggcgcata acggcctgct ggatggcgat agggagacgg gatgttttca tagcggcaga
    20581 ggatagagcg aagaaaagat gactgcaaga ggtggcttcc tccgcgatgg gaggaagcct
    20641 gaggagatta atcgtgagcg ccgatttcgc gcagtttacg acctttcatc agattacgtt
    20701 caatatgttc cagcgaaacg tgtttggttt ccggtaccag ccacaatgtc agcaggataa
    20761 acagtacgtt cagagccgca tacacccaga aggtgttggc gttacccagc gtgttgagca
    20821 tggtcaggaa cgttgcgcca acgatcatgt tggcaatcca gttggtggcg gtggagcagg
    20881 tgatgccaaa atcgcggcct ttcagcggct gaatttcgga gcacagtacc caaatcagcg
    20941 gaccggcact catggcaaaa ccgacaataa acatcagcag catggcgatg gcgaaatact
    21001 gcgctgacgg agagtgaata ccgatatgca tcattgtacc gagtacgccc atgccagcag
    21061 ccatcaccag gaagcccagc gttagcgttg gtttacgtcc ccagcggtca acaaggccga
    21121 ttgcgataaa ggtggcaagt acgttggtca ggccgacaat cacggtcccc cacatttgct
    21181 cggtagtgtt ggtataaccc gccagttcga agattttcgg cgcgtaatac atgatgacgt
    21241 tcatcccggt gaattgctgc attacctgca acagtacgcc aaggaacacc gcgcggcgga
    21301 agttgctgtt ctctttaaac agcgcccagc cactctgttt aacctgcaaa ctttcacgga
    21361 tttcatccag ttcgcgtttc gcttccgcgc tggtgtcacg caggcgtagc agcacgcgtt
    21421 cggcatcaac aaaacggcgc ttggcggcaa accaacgtgg gctgtctggc aggaagaaga
    21481 caccaatcag cagcaaaatt gccgggatga taatcacacc cagcatccag cgccatgcac
    21541 cggtgtagct gaaggcggta tcagaaagat aagcaccgag gatcccgata gtgatcatca
    21601 actgatacat cgagatcata ctgccgcgaa ttttttccgg cgcaatttct gacaggtaca
    21661 gtggtgcggt ataagaggcc acacccaccg ccagccccag cagaacgcgg gaaagaatca
    21721 gtacttcaac gtttggcgca gccgcagaga acagcgaacc ggcaacaaac aaaattgcgc
    21781 cgatcatcag gctctttttg cgcccgagtt taaaggagag ccagccgctg cccaccgcac
    21841 cgactgccgc accgaacatc atggagctta cgacccattc ttgcgtgtgc gaagtaatct
    21901 ggaattcatc tgcaataaac ggcagtgcgc cagcaattac accgatatcc aggccaaaga
    21961 gtaatcccgc cagagcggca aggaagcaga cgaaaaaagt cattgccttg tttgaccgcc
    22021 cctgtttttt agcgtcaggc atgatgccct ccaatatggt tattttttat tgtgaattaa
    22081 gataggtgag tacgacgtaa aaagatgtga agcaaatcac atcagtgtaa tcgcttacac
    22141 taacatttat cttagttatg gtttaagtgc atgaaaataa ttgagttata tcatcataga
    22201 ttaaacgctg ttatctgcaa ttaagacttt actgaaaaga aatgtaacaa ctgtgaaaac
    22261 tgaataataa tatgaaaata ttacaaatat ttggctggtg ttattggtgt aatcggtttc
    22321 agtagtgatg ataatttgcg ataaaacgtg ttgaggaaga atgatgttat tgtgcgggca
    22381 gctttttgcc ggatgcggcg tgaatgccta tccaccgcgg tgaggtaaaa gaaaaaggcc
    22441 agcgcgaggc tggcctttga aagcaggtga agaaagatta cttcagaccg gcagcatcgc
    22501 gcagcagctg cgctttgtcg gttttttccc acgggaaatg ttcacgacca aagtgaccgt
    22561 atgctgcggt ttctttgtag atcgggtgca gcagatccag catctgaatc agaccgtatg
    22621 ggcgcaggtc gaagaactca cgtaccagca gagtcagttg ttcagaaggc actttctcgg
    22681 taccgaaagt ttctaccatg atggaggtcg gttcagccac gccgattgcg taggaaacct
    22741 gaatttcaca acgatcggcc aggccagcag caacgatgtt ttttgcaaca taacgtgctg
    22801 cgtacgcagc ggaacggtcc acttttgatg gatctttacc agagaatgct ccgccaccgt
    22861 gacgtgccat gccgccgtag gtatcaacga taattttacg accagtcaga ccgcagtcac
    22921 ccatcgggcc accgataaca aaacgaccgg tcgggttgat gaagaatttg gtggcagaag
    22981 tcagccattc agcgggcaga atcggcttga tgatctcttc cattaccgct tcttgcagcg
    23041 atttctggtc gatctcttca gagtgctgag tggaaagcac gacagcatcg ataccaacga
    23101 ttttgccgtc gtcatactgg aaagtcacct ggcttttcgc gtccgggcgc agccacggca
    23161 gagtgccgtt tttacgcact tcagcctgac gctgtaccag acggtgtgca taggtgatag
    23221 gtgctggcat cagcacgtcg gtttcattag ttgcgtagcc aaacatcaga ccctggtcac
    23281 ccgcgccctg ttccagcgga tcggcacggt caacgccctg gttgatgtca ggagactgtt
    23341 tgccgatagc gctcagaacc gcacaggagt tagcgtcaaa gcccatgtcg gaatgcacat
    23401 agccaatttc gcgaacggtg ttacgggtga tctcttcgat gtctacccag gcgctggtgg
    23461 tgatttcgcc gccaactaaa accatgccgg tttttacgta ggtttcgcaa gcaacgcgtg
    23521 ctttcggatc ctgttcgagg atcgcgtcta aaacggcatc agagatttgg tcagcaattt
    23581 tgtcaggatg cccttcagag acggactcgg acgtaaaaag gtgttttgcc atatttaata
    23641 tcacctaaag agaatttggt tagctcaaac tgttgtgtgg attttctgtg gtagcggatc
    23701 ctaccacgac tctgcaagtt aaaaacactg gcagtctgag tgttaatcgg tatggatgga
    23761 ttaacatctg gatggctatt ttaggtcaat tcttcacccg atttccactt ttttttgaat
    23821 cgtgtctcat tctgttaaaa acgtggctgg aaatttttcc tgacaatgcc ggcattctgc
    23881 gtatttatct tttgcaattt tctgccattg tggggtataa aacgcggcgc gcggcttaaa
    23941 taaaaagcac acgacgtttc tttcgtgttg ccacttccag ccgggttgaa atcagagttt
    24001 tggcttgtgg gttcgtctta acaggcggcc gtggaggtga tacgaaataa tgaaccgttg
    24061 tctgctgctt aacctgtctc accgttctgg tgaagattcg ttccccgcac tctgcatctc
    24121 tgctttgcat acctgccgat gttataccca tctcggcgct tctcaggatt caagagctgg
    24181 ttacagttac tgaggactga acaagggcgc tcttgtaaaa acaagagttt tctcgtggtt
    24241 tcgccgaact gtcacactta cgttcggtta tgtgcttaat aatgttatga aaaagaaacc
    24301 ggttgcgcag ttggagcgtc agcattcact gctggaaaat ccatgtgctt atgggttgtt
    24361 atcgcagttc caggctgcga tagtcgttaa ctgttttaca cttaataaaa taatttgagg
    24421 ttcgctatgt ctgacgacat gtctatgggt ttgccttcgt cagcgggcga acacggtgta
    24481 ctacgctcca tgcaggaggt tgcaatgagc tcccaggaag ccagcaagat gctgcgtact
    24541 tacaatattg cctggtgggg caataactac tatgacgtta acgagctggg ccacatcagc
    24601 gtgtgcccgg acccggacgt cccggaagct cgcgtcgatc tcgcgcagtt agtgaaaact
    24661 cgtgaagcac agggtcagcg tctgcctgca ctgttctgtt tcccacagat cctgcagcac
    24721 cgtttgcgtt ccattaacgc cgcgttcaaa cgtgcgcggg aatcctacgg ctataacggc
    24781 gattacttcc ttgtttatcc gatcaaagtt aaccagcacc gccgcgtgat tgagtccctg
    24841 attcattctg gcgaaccgtt ggggctggaa gcaggctcga aagcagagtt gatggcggtg
    24901 ctggcgcatg ctggcatgac ccgtagcgtc atcgtctgca acggttataa agaccgcgaa
    24961 tatatccgcc tggcattaat tggcgagaag atggggcaca aggtctatct ggtcattgag
    25021 aagatgtcag aaatcgccat tgtgctggat gaagcagaac gtctgaatgt cgttcctcgt
    25081 ctgggcgtgc gtgcacgtct ggcttcgcag ggctcgggta aatggcagtc ctccggcggg
    25141 gaaaaatcga agttcggcct ggctgcgact caggtactgc aacttgttga aaccctgcgt
    25201 gaagccgggc gtctcgacag cctgcaactg ctgcacttcc acctcggttc gcagatggcg
    25261 aatattcgcg atatcgcgac aggcgttcgt gaatccgcgc gtttctatgt ggaactgcac
    25321 aagctgggcg tcaatattca gtgcttcgac gtcggcggcg gtctgggcgt ggactatgaa
    25381 ggcacccgtt cgcagtccga ctgttcggtg aactacggcc tcaatgaata tgccaacaac
    25441 atcatctggg cgattggtga tgcgtgtgaa gaaaacggtc tgccgcatcc gacggtaatc
    25501 accgaatcgg gtcgtgcggt gactgcgcat cacaccgtgc tggtatctaa tatcatcggc
    25561 gtggaacgta acgaatacac ggtgccgacc gcgcctgcag aagatgcgcc gcgcgcgctg
    25621 caaagcatgt gggaaacctg gcaggagatg cacgaaccgg gaactcgccg ttctctgcgt
    25681 gaatggttac acgacagtca gatggatctg cacgacattc atatcggcta ctcttccggc
    25741 acctttagcc tgcaagaacg tgcatgggca gaacaactct atctgagcat gtgccatgaa
    25801 gtgcagaagc aactggatcc gcaaaaccgt gctcatcgtc cgattatcga cgagctgcag
    25861 gaacgtatgg cggacaaaat gtacgtcaac ttctcgctgt tccagtcgat gccggatgcg
    25921 tgggggatcg accagttgtt cccggttctg ccgctggaag ggttggatca agtgccggaa
    25981 cgtcgcgctg tgctgctgga tattacctgt gactctgacg gtgctatcga ccactatatt
    26041 gatggtgatg gtattgccac gacaatgcca atgccggagt acgatccaga gaatccgccg
    26101 atgctcggtt tctttatggt cggcgcatat caggagatcc tcggcaatat gcacaacctg
    26161 ttcggtgata ccgaagcggt tgacgtgttc gtgttccctg acggtagcgt agaagtagaa
    26221 ctgtctgacg aaggcgatac cgtggcggac atgctgcaat atgtacaact cgatccgaaa
    26281 acgctgttaa cccagttccg cgatcaagtg aagaaaaccg atcttgatgc tgaactgcaa
    26341 caacagttcc ttgaagagtt cgaggcaggt ttgtacggtt atacctatct tgaagatgaa
    26401 taattaagat tattccgctg gcgggtcgat ttatttacgt aaatcggccc gctttctttt
    26461 tatatattct taaaaataat ctcgctgctt ttcttattaa ataatctccc tacaatcgct
    26521 gcattaatta aatgcagagg gaacggttat gaaaaaatgg aaagtacgta gcgcgttggt
    26581 ggcattaatc gtgctgctgg ccggttgttc cagtaatgcg cagtataata gcagtgcatc
    26641 gggcaatgtc ggcactgcct ggggtgggga tgtgcactca acggttcagg gcgtatctgc
    26701 tgagcgagcc tggcgcgatc cggcagagat gatcgttatt agttattcga ctaatgttcc
    26761 ttccggctac gaccgcgttt atagcattcg tattaacgaa ctggaatatg ctattcgcga
    26821 tggtaatttt aattctctac ctattacgcg ggtttatgat tcatccaata atgaaccacg
    26881 ctatattgtc catgccagag tcggaatgaa ttatcaatta tatgtccgta attacagtcg
    26941 taacaccaac tacgagatcg ttgctacggt tgatggcatg gatgtgctga acggcaagca
    27001 aggttcgctg aataacaacg gctatattgt gaatgccgga gattcgttag cgattaaagg
    27061 attccgcaaa gataaacata ccgaagcggc ttttcagttc gccaacgttg ctgattccta
    27121 cgcagccaat tcggcgcaag gcgatgtgcg taataccggg gtaatcggct ttgccgcctt
    27181 tgaattacaa ggcccggcgc aaaacgcgct gccgccgtgc tctggacaag cctttccggc
    27241 agataacaat ggctatgcgc cgccaccgtg tcggaaatag caacctcgct tgatgcctcg
    27301 cccatttaag gcgataatta gcacaaactt gtgtttcgca aatccaaccc ttcctcgtcg
    27361 ggcctaacga cgcggaaggg tttttttata tcgactttgt aataggagtc catccatgag
    27421 caccttaggt catcaatacg ataactcact ggtttccaac gcctttggtt ttttacgcct
    27481 gccgatgaac ttccagccgt atgacagcga tgccgactgg gtgatcaccg gcgtgccgtt
    27541 cgacatggcc acctctggcc gtgccggtgg tcgtcacggt ccggcagcga tccgtcaggt
    27601 ttcgacgaat ctggcctggg aacacaaccg cttcccgtgg aatttcgaca tgcgtgagcg
    27661 cctgaacgtc gtggactgcg gtgatctggt atatgccttc ggcgatgccc gtgagatgag
    27721 cgaaaaactg caggcacacg ccgagaagtt gctggctgcc ggtaagcgta tgctctcttt
    27781 cggtggtgac cactttgtta cgctgccgct gctgcgtgct catgctaagc atttcggcaa
    27841 aatggcgctg gtacacttcg acgcccacac cgatacctac gcgaacggtt gtgaatttga
    27901 ccacggcacc atgttctata ccgcgccgaa agaaggtctg atcgacccga atcattccgt
    27961 gcagattggt attcgtaccg agtttgataa agacaacggc tttaccgtac tggacgcctg
    28021 ccaggtgaac gatcgtagcg tggatgacgt tatcgcccag gtgaaacaga ttgtgggtga
    28081 tatgccggtt tacctgacct ttgatatcga ctgcctggat cctgcttttg caccaggcac
    28141 cggtacgcca gtgattggcg gcctgacctc cgatcgcgct attaaactgg tacgcggcct
    28201 gaaagatctc aacatcgttg ggatggacgt agtggaagtg gctccggcat atgatcagtc
    28261 ggaaatcacc gctctggctg cggcgacgct ggcgctggag atgctgtata ttcaggcggc
    28321 gaaaaagggc gagtaagcac cagatgcgat gcgcacgggt aaaacgtgcc attaatgtcg
    28381 gatgcggcgt gaacgcctta tccgacctac gttcggcacc cgtaggccgg ataagatgcg
    28441 ccagcatcgc atccggcaat gcgcacaagg taacaaatgt gccattcatg tcagatgcgg
    28501 cgtgaacgcc ttatctgacc tacgttcgac accaccaggc tttacttaac cccatccgcg
    28561 ctcatacgat cgcgaatatg ctgggcgcgt tcggcggatg ccggatggtc gtcaaacatt
    28621 gagctttggc ggccttcttc cagtttcgcc agtttttcaa agctggtggc aagacctgcc
    28681 gggctgatgc cgcgttggcg tagaagatcg taagaataat catcggcttc tgcttcctgg
    28741 cgctgggaga attgcgaatt gactaatttc tcgcccagat caccaagttg tgattgagat
    28801 aaactgccga caatcccgcc cgcagaggcc gcagctactc gcacggcatt tgtaccaagc
    28861 gccacctgca tccctttctt cacatggcct aacgccacgt gccccatctc gtgaccgatc
    28921 accgcttcga cttcgttatc cgtcatcata tccatcagcc cgctatagac gcggatacag
    28981 ccgttagcca ttgcaaaggc gttcacatcc ttcgccatat acactttgta attcaccggc
    29041 tgaccgttga tattgttgcc tagcgcattg gcaatagttg tcagacgttt agcgtattcg
    29101 ctattggctg gcgcaatcgt cgccttgctg tccatctcct gacatgcctg atcgctcagg
    29161 gctttcacct gcgcatcact caaactgtaa gcctgaaaag cttccgctcc tgatgaaagc
    29221 agtccgttgg agtccatatt ctggcaaccg gtcagtaccg ttgccacgct cattgctacc
    29281 aataaggcgc gaattttcat gttttctctt ccgcacaagg tcaggattat tctaaaaatg
    29341 cgccgtttgc aggtgaatcg acgctcagtc tcagtataag gaaaagcgca gcggacgggc
    29401 gagtagattg cgcaacatgc gagcatgatc cagagatttc tgaagcggca aaaggatgtt
    29461 ccatgtacat gacgcgcggc ttgcggtaaa ttgttggcaa attttccggc gtagcccaaa
    29521 acgcgctgtc gtcaagtcgt taagggcgtg cccttcatca tccgatctgg agtcaaaatg
    29581 tcctcacgta aagagcttgc caatgctatt cgtgcgctga gcatggacgc agtacagaaa
    29641 gccaaatccg gtcacccggg tgcccctatg ggtatggctg acattgccga agtcctgtgg
    29701 cgtgatttcc tgaaacacaa cccgcagaat ccgtcctggg ctgaccgtga ccgcttcgtg
    29761 ctgtccaacg gccacggctc catgctgatc tacagcctgc tgcacctcac cggttacgat
    29821 ctgccgatgg aagaactgaa aaacttccgt cagctgcact ctaaaactcc gggccacccg
    29881 gaagtgggtt acaccgctgg tgtggaaacc accaccggtc cgctggggca gggtattgcc
    29941 aacgcagtag gtatggcgat tgcagaaaaa acgctggcgg cgcagtttaa ccgtccgggc
    30001 cacgacattg tcgaccacta cacctacgcc ttcatgggcg acggctgcat gatggaaggc
    30061 atctcccacg aagtttgctc tctggcgggt acgctgaagc tgggtaaact gattgcgttc
    30121 tacgatgaca acggtatctc tattgatggt cacgttgaag gctggttcac cgacgacacc
    30181 gcaatgcgtt tcgaagctta cggctggcac gttattcgcg acatcgacgg tcatgacgcg
    30241 gcatccatca aacgcgcagt agaagaagcg cgcgcagtga ctgacaaacc gtccctgctg
    30301 atgtgcaaaa ccatcatcgg tttcggttcc ccgaacaaag ccggtactca cgactcccac
    30361 ggtgcgccgc tgggcgacgc tgaaattgcc ctgacccgcg aacagctggg ctggaaatat
    30421 gcaccgttcg aaatcccgtc tgaaatctat gctcagtggg atgcgaaaga agcaggccag
    30481 gcgaaagaat ccgcatggaa cgagaaattc gctgcttacg cgaaagctta tccgcaggaa
    30541 gccgctgaat ttacccgccg tatgaaaggc gaaatgccgt ctgacttcga cgcgaaagca
    30601 aaagagttca ttgctaaact gcaggctaac ccggcgaaaa tcgccagccg taaagcgtct
    30661 cagaatgcta tcgaagcttt cggtccgctg ttgccggaat tcctcggcgg ttctgctgac
    30721 ctggcaccgt ctaacctgac cctgtggtct ggttctaaag caatcaacga agatgctgcg
    30781 ggtaactaca tccactacgg tgttcgcgag ttcggtatga ccgcgattgc taacggtatc
    30841 tccctgcacg gtggtttcct gccgtacacc tccaccttcc tgatgttcgt ggaatacgca
    30901 cgtaacgccg tacgtatggc tgcgctgatg aaacagcgtc aggtgatggt ttacacccac
    30961 gactccatcg gtctgggcga agacggcccg actcaccagc cggttgagca ggtcgcttct
    31021 ctgcgcgtaa ccccgaacat gtctacatgg cgtccgtgtg accaggttga atccgcggtc
    31081 gcgtggaaat acggtgttga gcgtcaggac ggcccgaccg cactgatcct ctcccgtcag
    31141 aacctggcgc agcaggaacg aactgaagag caactggcaa acatcgcgcg cggtggttat
    31201 gtgctgaaag actgcgccgg tcagccggaa ctgattttca tcgctaccgg ttcagaagtt
    31261 gaactggctg ttgctgccta cgaaaaactg actgccgaag gcgtgaaagc gcgcgtggtg
    31321 tccatgccgt ctaccgacgc atttgacaag caggatgctg cttaccgtga atccgtactg
    31381 ccgaaagcgg ttactgcacg cgttgctgta gaagcgggta ttgctgacta ctggtacaag
    31441 tatgttggcc tgaacggtgc tatcgtcggt atgaccacct tcggtgaatc tgctccggca
    31501 gagctgctgt ttgaagagtt cggcttcact gttgataacg ttgttgcgaa agcaaaagaa
    31561 ctgctgtaat tagcatttcg ggtaaaaagg tcgcttcggc gacctttttt attaccttga
    31621 tatgtccgtt tgcggacaag caatagataa agcgtgttgt agatcacaaa tatttatatg
    31681 caataaatat caattatgta atatgcatca cgatatgcgt attgacattt gttgttatat
    31741 ctataactca atgttatata agaaattaat aattcactgt tttcaaaaca tcggtttccc
    31801 tgctcaattg ctttcattaa accgcacgag tattttattt ttaccgccgg acggtaccct
    31861 aaaaataaaa taaggataat ttatgcggct tagtgattat tttccagaat catcaatctc
    31921 agttatatat tcagcaaaag attggcagga agctatcgat ttctcgatgg tatcattgct
    31981 ggataaaaac tatatcagcg agaattacat tcaggcaatt aaagattcca ccattaacaa
    32041 tggcccttat tatattctcg caccaggcgt ggcaatgcct catgcgcgac cggaatgtgg
    32101 ggcgcttaaa accgggatgt ctttgacatt acttgaacaa ggtgtttatt ttccggggaa
    32161 tgacgaacca attaaattac tcatcggact ctctgctgcc gatgccgatt cgcacattgg
    32221 cgctattcag gcgttaagtg aattactgtg cgaagaagaa atactcgaac aactcttaac
    32281 agcatcatca gaaaaacaat tagcggacat tatcagccgc ggataattac tctctcctct
    32341 acaggataat attatggaaa acaagtctgc tcgtgcaaag gtccaggctt ttgggggctt
    32401 tttgactgca atggtcatcc ccaatattgg tgcttttatt gcctggggct tcataactgc
    32461 gttatttatt cccaccggtt ggctgccgaa tgaacatttc gccaaaattg tcggcccgat
    32521 gattacctat ttattgcccg tgatgattgg ttctactggt ggtcatctgg tcggcggtaa
    32581 acgcggggcg gtcatgggcg gaataggtac tattggtgtg atcgttggcg cagagatccc
    32641 gatgttcctt ggctcaatga tcatggggcc gctcggtggg ttggtcataa aatatgtcga
    32701 taaggcactg gaaaaacgca tacctgccgg ttttgagatg gttatcaata acttctcatt
    32761 aggtatcgcg gggatgctcc tttgtctgct gggttttgaa gtgatcggcc cggcggtgtt
    32821 aattgccaat actttcgtca aagagtgtat cgaggcgctg gtacatgcgg gttatctgcc
    32881 tctgctgtca gtcatcaatg aaccggcgaa agtgcttttc cttaataatg cgatcgatca
    32941 gggcgtctat tatccgctgg gaatgcaaca ggcttcggtt aacggtaaat ccatcttctt
    33001 tatggtggcc tctaacccag gtccgggcct ggggctgctg ctggcgttta ccttgtttgg
    33061 taaagggatg agtaaacgtt ctgcgcccgg ggcgatgatt attcacttcc tcggtgggat
    33121 ccacgaactg tatttcccat atgtgctgat gaagccgctg actattattg ccatgattgc
    33181 gggcggtatg tctggtacct ggatgtttaa cttactggac ggtggtctgg tggctggccc
    33241 aagtccgggg tctatctttg cttacctggc actgacgccg aaaggctcgt tcctggcgac
    33301 aattgccggt gttacggtag gtaccctggt gtcctttgct attacttcgc tgatactgaa
    33361 gatggaaaaa acggtggaaa cggagagcga agatgagttt gctcagtcag ccaatgcggt
    33421 taaggcgatg aaacaagagg gtgcattctc gttaagcagg gttaagcgta tcgcctttgt
    33481 ttgcgatgcg gggatgggct ccagtgcgat gggcgcgacc accttccgta aacgcctgga
    33541 aaaagcgggg ctggcaatag aagtaaaaca ttacgcgata gaaaacgtgc ctgcggatgc
    33601 cgatatcgtc gttactcatg ccagtctgga agggcgcgtg aaacgtgtta cggataaacc
    33661 gctgatattg attaataact atattggcga tccaaaactc gacactttat ttaatcaatt
    33721 aaccgccgaa cataaacact gattacagag gtaaaaatga aaaccaaagt tgctgctatt
    33781 tatggcaagc gggatgtccg tctgcgcgaa tttgaactgc cagaaattac cgataatgaa
    33841 ctgttagtga gtgtaatttc tgacagcgtc tgtttatcga cctggaaagc ggcgttactc
    33901 ggtagtgaac ataaacgcgt acccgacgat ttagaaaatc atccggtcat taccgggcat
    33961 gaatgtgccg gggttattgt cgaagtgggt aaaaatctca ctggcaaata taaaaaaggc
    34021 cagcgttttg tattgcaacc ggcgatgggg ttaccaagcg gatattcagc aggctacagc
    34081 tacgaatatt ttggcggtaa cgccacctat atgattattc ccgaaatcgc cattaatttg
    34141 ggctgcgtat taccgtatca cggctcttat tttgctgcgg cgtcgctggc agagcctatg
    34201 tgctgcatta ttggtgctta tcatgccaat tatcacacca cgcaatatgt ttatgagcat
    34261 cgcatgggcg tcaaacctgg cggcaatatt gcactgctgg cgtgtgcggg tccgatgggc
    34321 attggcgcta tcgattacgc cattaacggt ggcatacaac cgtcacgggt ggtggtggtc
    34381 gatatcgacg acaaacgcct ggcgcaggtg cagaagctgc tgccggtgga tctggcggcc
    34441 agtaaaggca ttgagctggt gtatgtgaat accaaaggga tgagcgatcc ggtccagacg
    34501 ctgcgggcgc tgacaggaga tgtcgggttc gatgacattt ttgtttatgc ggcggtgcct
    34561 gctgtcgttg agatggctga cgaattactg gcggaagatg gctgtctgaa cttctttgcc
    34621 gggccgacgg ataaaaactt caaagtgccg tttaatttct acaacgtcca ttacaacagc
    34681 acgcacgtag tcggtacatc cggcggttca acggacgaca tgaaagaggc gattgcgtta
    34741 agcgccacgg ggcagttgca gccgtccttt atggtcacgc atatcggtgg gctggatgcg
    34801 gtgccagata ccgtgctcaa tctgccggat atccctggcg gtaaaaaact catttataac
    34861 ggcgtgacca tgccgctcac tgccattgcc gattttgccg aaaaaggcaa aaccgatccg
    34921 ctgtttaaag agttggcgcg gctggttgag gaaacgcacg gcatctggaa tgaacaggcc
    34981 gagaaatatc tgctggcaca atttggcgtt gatatcgggg aggccgcgca atgatgtccc
    35041 tggcgtggcc attatttcgc gttacggaac aggcagcgtt ggctgcctgg ccgcaaaccg
    35101 gatgtggcga caaaaataaa attgatggcc tggcggtcac cgcgatgcgc caggcattaa
    35161 acgacgtcgc ttttcgtgga cgagtggtta tcggcgaagg agaaattgac catgcgccga
    35221 tgctgtggat tggcgaagag gtgggcaaag gcgatgggcc agaagtcgat atcgcggttg
    35281 acccgattga aggcacgcgg atggtagcga tggggcagag caatgcgctg gcggtcatgg
    35341 ctttcgcccc tcgagatagc ctgttacatg cgcccgatat gtatatgaaa aagctggttg
    35401 ttaatcgact ggctgccggg gcgattgatc tgtctttgcc tctggcggac aacttgcgta
    35461 acgtggcgcg ggcgttaggt aaaccgctgg acaagctgcg catggttacg ctggataaac
    35521 cgcgcctgag tgccgcgatt gaagaagcaa cacagctggg cgtgaaggtt ttcgctctgc
    35581 cggatggcga tgtcgccgcc agcgtgctga cctgctggca ggataatcct tacgacgtga
    35641 tgtacaccat cggcggtgcg ccagaaggcg tgatttctgc ctgtgccgtt aaagcattag
    35701 gtggggatat gcaggccgag ctgattgact tttgtcaggc taaaggggat tacacggaaa
    35761 atcggcagat tgcagagcag gagcgcaagc gttgtaaggc aatgggcgtt gacgttaacc
    35821 gtgtgtactc gctcgatgaa ctggtgaggg ggaacgatat cctctttagc gctacgggcg
    35881 tgacgggcgg cgagttggtg aacggtatcc aacagacggc gaatggggtg cggacgcaga
    35941 cattactgat cggcggcgcg gaccaaacgt gtaatataat agactccctg cattgatggt
    36001 gatctatcgg aagaccaatg gcgacgctga cagaagatga tgtgcttgag caactggatg
    36061 cacaggacaa tttattttca tttatgaaaa ctgcgcattc tattttgctc caggggatac
    36121 gccagtttct gccgtcgctg tttgtcgata acgatgaaga gatcgtcgaa tatgcagtga
    36181 agccgttact cgcccaaagc ggcccgcttg acgatattga tgttgcgctg cgtttgattt
    36241 atgcgctggg gaaaatggat aaatggctgt atgccgatat cacgcacttt tcccagtact
    36301 ggcattacct gaacgaacag gatgaaacgc ccggatttgc cgatgacatg acctgggatt
    36361 ttatcagcaa cgtcaatagc ataacctgca atgcgacgct ctatgacgcg ttaaaagcga
    36421 tgaagttcgc cgatttcgcc gtctggtcag aggcgcgttt tagcggaatg gtcaaaacgg
    36481 cgctgacgct ggcagtaacg accaccttaa aggaattaac gccgtgaaaa ttgaattaac
    36541 ggtgaatggg ctgaacgtgc aagcgcagta ccatgatgaa gagatagaac gcgtgcataa
    36601 accgttgctg cgtatgctgg cggccttaca gacggtaagt ccgcagcgac gtacggtggt
    36661 ttttctttgc gctccgccgg ggacgggtaa atctacctta actaccttct gggaatatct
    36721 cgcgcaacaa gaccctgaat tacccgctat ccagacgctc ccgatggatg gttttcacca
    36781 ttacaatagc tggctggatg cgcatcaatt gcgccccttc aaaggcgcac cagagacatt
    36841 tgacgttgcg aaactggcgg aaaatctgtg ccaggtagtg gaaggggatt gtacgtggcc
    36901 gcagtatgat cgacaaaagc atgatcctgt tgaagatgcg ttgcacgtta ccgcaccact
    36961 cgtcatcgtc gaaggaaact ggttgttgct ggacgacgag aagtggtgcc agcttgcgca
    37021 attttgtgat ttctcgatct ttatcaacgc gccagctact gcgttgcggg aacgactggt
    37081 agggcgcaag ctggcgggag gggtatcgct ggcagacgca gaagcttttt atgaccgtac
    37141 cgacgggccg aatgttcgcc gggtgctgga agagagtctg cctgcaaatt tgaccttaat
    37201 gatgaccgct acgggtgaat atcgtctggt ggattaacgt tcctgattag accatcttcc
    37261 agtcgcacca cacgagagaa atgacgccgg gtaaatgcgg cgtcatgact gattgctacg
    37321 acgctcgtgc ctcgctggcc gcacttctcc agccagcttt caaacacgct caaccaattt
    37381 tcgtcaaaat cccgactcgg ctcgtccagc agcaatagtg gcggagagag tgcttccagg
    37441 caggcgacgg cgaccatccg tcgctgcgca ctatgcagat ccaacgggtg cgagttggca
    37501 gtgtctgcta actgacaaca ttgcagggca gcatgggtac gttgagtgat ttcatccgcc
    37561 gggcattttt gcagtttcaa accaaatgcg atctcgtcag cgacggtgct gtgaaaaagt
    37621 tggttttccg cttcctgaaa cagtacgcca acctttgccg cgcgctggcg gtttttcagg
    37681 tttttcatcg cctgttgttg cagcataacc gtaccggctg tgggcgtaag aagacctgcc
    37741 attactcgta acagcgtcga ttttcccgcg ccattgtcgc cggtcagcgc caaccattca
    37801 ccttgtttga gttgcagaga aatgtcgcaa agacaatccg ttgccgcgcc aggccagcga
    37861 taagatatct tatttaacgt taacatagtg gctggagcgc tccgttttgc aattgccacg
    37921 cttgctggca gtagttcagg aaaggtgtgc gatgacgttc aaaaagaata atcagcgaac
    37981 cacgttcaaa cgcccagtgt tgtaagcgct gcaacagcat ttcgctggcc tgcggagtca
    38041 ggcggctaaa ggcttcatca agaattaaga gctttggctg catcgcgatg gcgcaggcga
    38101 tgaccacgcg ctgtgtctct ccaccggaga gtgttgccgg gtgacgatgg cgcaacggct
    38161 ggcactcggt cagagtcagg gcggcatcaa tccgcgccat aatctctgct tcagcaagac
    38221 acagattttc tggcccaaag gcaacttctt cttccacgct aaaggtacag ccggagagct
    38281 gcaaataggg cgattgctgg acgagctgga tggtggcgga ttgttcatta agcgggagat
    38341 gcccaatggg cgtaccgagg agcgtccccg tgcctgttat ctctcctggc agaaagtccg
    38401 gataccagcc cgccatcagc tgtgccagcg tacttttgcc actgccgttg tcaccaaaga
    38461 tggcgaccat ccccggtttg acatagtgaa agtcataaca gaacggagga tgcgttgagt
    38521 gtgtcgggca ataacggaac tgttctaacg taaccatatc cacgctccga attctgtcag
    38581 catcagcaag atcatggtgt aacgcgctac gcgttgtagg gtgctatcgg ctggtgccca
    38641 caacgttgtt cgtcggttgt ggatccgaaa tgcccgcata tccagcgccg caccacggac
    38701 agccagatcg ttaagcgcat tgtgtgttag cggaataatt aacgcgggca tcgcacgtaa
    38761 acgctgatac cagccctcat ccagcggcac gccgcgggca cgctgcgctt catgaataat
    38821 cgctaattgc cgttttaact gctcaacgac caataacggc ccggcaaaca gataagcgac
    38881 gcctggcggc aggcgagaag caaacagagc gcgaataaaa cattgcacgg ggacgtactg
    38941 catccacaac tgtgaggttg aaacaatcgc cagaatccgc aaccagagcg taatagcatg
    39001 cgaccaccgt tctggcgaac gcggcgtgcc gcttagccat tctgttagcc agccaccgtg
    39061 taccagccac agcccggctc ccagcgagaa catcagccag acgacatatt ttgcccgccg
    39121 ccgcgtggcc tttagcgcaa taaggcaaaa aaaggtcgcc gcgctataga tcggcaaaat
    39181 ggtctgcgcg ggcaggataa gcgtggtgca ggccgccaat gcccatagtg ttaatgaggt
    39241 aaatggatgc atttagcgca cggcagccat tgccggaaaa tggcgcgtgg tgcgaatcgg
    39301 cagctggcgc agtaaaagcc agacaatcac cgctgtaaga attttatcga ccaggttcgc
    39361 gcctatcacc gtaatcgcca ccgactccac caggttttgc cccatagagt gcatccaggc
    39421 aacgaacaaa tcggctccgc taccggtgac accgccgaac agcgcagtac gcagcggtac
    39481 agcgacgacc gttaccgcaa gcgtaataat cacgccgctg acaacgactt ttggcagggt
    39541 gcgaaaccaa cccgctcgcg ccagccaacc ggcgaccagg ccgatgacca tcgcgacggg
    39601 ggcaaatgcg gcggcgatcg ggtcggtcag caatccccag agtaaattgg tcagtaagcc
    39661 ggttagcatc ccgatcaccg gaccaagcag taccgcgcta ataagggtgc cgatggagtc
    39721 gagaaatata ggtaatttga ccatgctggc cagttgcccg ccgatcatat tgatggcgat
    39781 agatataacg atcaacacca gtgcctgact ggaaaagtgg ctacgcgcca taagaaaacc
    39841 tctctgaaag tagttattta acgggggaaa tatgcaactc gtcgtaacca gaaagcgcgc
    39901 aaggttggcg gctaaacgcc agttcctcaa ggctaccgat cactagttca agcgtggtcc
    39961 gcacggtaca gccgggcatc atatgaccgc caagcatcgt gccgtgtgga tcggaaacgc
    40021 agagatgaag atgctcacca ctctgttcca gcgtgccgtt tagtgagatc acttcgaatg
    40081 taccgttcag gagcgtggtg ccttcttgcc cggcatagcg caaggcgacg tcagtcaggc
    40141 taccggtaca gcctgcaatc caggcggcat gaagttgctg ctgctgtgca aaagcgtgta
    40201 gctgggaaaa aacttcctgc ccggggagca ggcgcagtgc gtaaaagcgt gcggaggaag
    40261 aatatgggta gggcaatgtc atcattggag tactccgaaa ctcggacgta ttgcggataa
    40321 tatcgacgct gcatcagtca tattctgata taagtcaggg tttgtgacgg cacaacgaaa
    40381 agattttgct acgcttattc gtgattctgc aaagtggaat aaattccaca ggggcggggc
    40441 tgaaaacgtg acgtaagtca gatatttgtt ttctttgact ggacaatcat tccttttatt
    40501 ccacgtttcg cttatcctag ctgaagcgtt tcagtcgatt aaatgttcga caattaacca
    40561 atcagtcgca gtttgcgaca ggtaaggttt ccccggacga tttgctggat tactctgtct
    40621 ggcaaatttg ctcagggaaa accttgcagg agatctatga ccgtacgcgt agcgataaat
    40681 ggcttcggtc gcatcgggcg taatgtggtt cgtgctttgt atgaatcagg acgccgtgcg
    40741 gaaattaccg tggtggcaat caacgaactg gcggatgctg cgggcatggc gcatttgttg
    40801 aaatatgaca ccagtcatgg ccgttttgca tgggaagtac gacaggaacg cgatcaactt
    40861 tttgttggtg atgacgccat ccgcgtattg catgaacgtt cactgcaatc gctcccctgg
    40921 cgtgaacttg gcgttgatgt agtcctcgac tgcaccggcg tatatggctc ccgcgagcat
    40981 ggcgaagcgc atattgccgc cggggccaaa aaagtgctct tttcacatcc tggcagtaac
    41041 gatctcgacg cgaccgttgt ttacggcgtc aatcaggatc aacttcgtgc ggaacaccgc
    41101 atcgtttcta acgcttcctg taccacgaat tgcataattc ccgtcatcaa attgttagat
    41161 gatgcgtacg gtattgagtc cggcactgtg accacaattc actccgccat gcacgatcaa
    41221 caggttattg atgcatacca tcctgacctg cgtcgcaccc gggcagccag ccagtcgatc
    41281 attccggtcg atactaaact ggccgccggt atcacacgat tttttccgca atttaacgat
    41341 cgctttgaag cgattgcggt acgtgtgcca accataaatg tgacggcaat cgatttaagc
    41401 gtgacggtga agaaacctgt aaaagccaat gaagtcaacc tgttgctgca aaaagcagca
    41461 caaggtgcat ttcatggtat agttgactat acggaattgc cgttggtctc tgtagatttt
    41521 aaccacgatc cgcacagtgc cattgtcgat ggcacccaaa cccgggtcag tggcgcacac
    41581 ctgatcaaaa cgttggtctg gtgcgataac gaatggggct ttgctaaccg aatgctcgac
    41641 acgacgttag ctatggctac tgttgctttc aggtaagacg caagcagcgt ctgcaaaact
    41701 tttagaatca acgagaggat tcaccatgtc tgtaattaag atgaccgatc tggatcttgc
    41761 tgggaaacgt gtatttatcc gtgcggatct gaacgtacca gtaaaagacg ggaaagtaac
    41821 cagcgacgcg cgtatccgtg cttctctgcc gaccatcgaa ctggccctga aacaaggcgc
    41881 aaaagtgatg gtaacttccc acctgggtcg tcctaccgaa ggcgagtaca acgaagaatt
    41941 ctctctgctg ccggttgtta actacctgaa agacaaactg tctaacccgg ttcgcctggt
    42001 taaagattac ctcgacggcg ttgacgttgc tgaaggtgaa ctggttgttc tggaaaacgt
    42061 tcgcttcaac aaaggcgaga agaaagacga cgaaaccctg tccaaaaaat acgctgcact
    42121 gtgtgacgtg ttcgtaatgg acgcattcgg tactgctcac cgcgcgcagg cttctactca
    42181 cggtatcggt aaattcgctg acgttgcgtg cgcaggcccg ctgctggcag ctgaactgga
    42241 cgcgctgggt aaagcactga aagaacctgc tcgcccgatg gtggctatcg ttggtggttc
    42301 taaagtatct accaaactga ccgttctgga ctccttgtct aaaatcgctg accagctgat
    42361 tgttggtggt ggtatcgcta acacctttat cgcggcacaa ggccacgatg tgggtaaatc
    42421 cctgtacgaa gctgacctgg ttgacgaagc taaacgtctg ctgaccacct gcaacatccc
    42481 ggttccgtct gatgttcgcg tagcaaccga gttctctgaa actgcaccgg ctaccctgaa
    42541 atctgttaac gatgtgaaag ctgacgagca gatcctggat atcggtgatg cttccgctca
    42601 ggaactggct gaaatcctga agaatgcgaa aaccattctg tggaacggtc cggttggcgt
    42661 gttcgaattc ccgaacttcc gcaaaggtac tgaaatcgtg gctaacgcta tcgcagacag
    42721 cgaagcgttc tccatcgctg gcggcggcga cactctggca gcaattgacc tgttcggcat
    42781 tgctgacaaa atctcctaca tctccactgg cggcggcgca ttcctcgaat tcgtggaagg
    42841 taaagtactg cctgcagtag cgatgctcga agagcgcgct aagaagtaaa aaatcacagg
    42901 gcagggaaac ctgcccttgt ttcagcgcgc ttttagagca cgcatttttt catactctaa
    42961 ataattcgag ttgcaggaag gcgacaagag agtgaatccc caggagctta cataagtaag
    43021 tgactggggt gagcgaacgc agacgcagca catgcaactt gaagtatgac gagtataagg
    43081 cccgacgata caggacaaga gacatgtcta agatttttga tttcgtaaaa cctggcgtaa
    43141 tcactggtga tgacgtacag aaagttttcc aggtagcaaa agaaaacaac ttcgcactgc
    43201 cagcagtaaa ctgcgtcggt actgactcca tcaacgccgt actggaaacc gctgctaaag
    43261 ttaaagcgcc ggttatcgtt cagttctcca acggtggtgc ttcttttatc gctggtaaag
    43321 gcgtgaaatc tgacgttccg cagggcgctg ctatcctggg cgcgatctct ggtgcgcatc
    43381 acgttcacca gatggctgaa cattatggtg ttccggttat cctgcacact gaccactgcg
    43441 cgaagaaact gctgccgtgg atcgacggtc tgctggacgc gggtgaaaaa cacttcgctg
    43501 ctaccggtaa gccgctgttc tcttctcaca tgatcgacct gtctgaagaa tctctgcaag
    43561 agaacatcga aatctgctct aaatacctgg agcgtatgtc caaaatcggc atgactctgg
    43621 aaatcgaact gggttgcacc ggtggtgaag aagacggcgt ggacaacagc cacatggacg
    43681 cttctgcact gtacacccag ccggaagacg ttgattacgc atacaccgag ctgagcaaaa
    43741 tcagcccgcg cttcaccatc gcagcgtcct tcggtaacgt acacggtgtt tacaagccgg
    43801 gtaacgtggt tctgactccg accatcctgc gtgattctca ggaatatgtt tctaagaaac
    43861 acaacctgcc gcacaacagc ctgaacttcg tattccacgg tggctctggt tctactgctc
    43921 aggaaatcaa agattccgta agctacggcg tagtgaaaat gaacatcgat accgataccc
    43981 aatgggcaac ctgggaaggc gttctgaact actacaaagc gaacgaagct tatctgcagg
    44041 ggcagctggg taacccgaaa ggcgaagatc agccgaacaa gaaatactac gatccgcgcg
    44101 tatggctgcg tgccggtcag acttcgatga ttgctcgtct ggaaaaagca ttccaggaac
    44161 tgaacgcgat cgacgttctg taagatattc ctttctgctt atctcaaggc ccgctctgcg
    44221 ggtctttttt tcgccaaaag caaaattgcc tgatgcgcta cgcttatcag gcctacacgt
    44281 atcctgcaat atattgaatt tgcaggtttt tgtaggccgg ataaggcgtt cacgccgcat
    44341 ccggcatgac aaagcgcaca ttgatatctg tctgagcccc gctctgcggg gattttttcg
    44401 ccaaaagcaa ggtgattcag atgagaaatc tgtgatctat ttggcaaaat tatgctttat
    44461 tgtttaccct tgtcagactg cccgtcataa ggcggcggag tgtatttctc cattttgagt
    44521 cagttgaaaa ggaatattga atggaagatt tgaatgttgt cgatagcata aacggcgcgg
    44581 gaagctggct ggtagctaac caggcgctgc tgctaagtta tgcagtaaac atcgtggcgg
    44641 cactcgcgat catcatcgtt ggtttgatta tcgcgcggat gatttccaac gcggtgaatc
    44701 gcctgatgat ctcccgtaaa atcgatgcca ctgttgctga ttttctttct gcattagtcc
    44761 gttacggtat tatcgccttt acgctaatcg ctgccctggg acgcgtgggt gtacaaacag
    44821 cgtcagtcat tgctgtactc ggtgccgcag gcttagctgt tggtctggct ttgcaggggt
    44881 cactttctaa cctggccgct ggcgtgttac ttgtcatgtt ccgcccgttc cgtgccggag
    44941 aatatgttga cctcggcggc gtagccggta ctgtgctgag tgtgcagatt ttctccacca
    45001 ccatgcgtac tgcagacggt aaaattatcg ttattccgaa cggtaaaatt attgccggaa
    45061 atattattaa cttctcccgc gagccagttc gccgtaacga atttattatt ggcgtggcgt
    45121 atgattccga tatcgatcag gttaagcaga tcctgaccaa tattatccag tctgaagatc
    45181 gcattttgaa agatcgcgaa atgactgtgc gcctgaacga acttggtgca tcgtcgatta
    45241 atttcgtggt ccgcgtctgg agcaacagcg gcgatctgca aaacgtgtac tgggatgtgc
    45301 tggagcgtat taaacgtgaa tttgatgccg ccggtatcag cttcccgtac ccgcaaatgg
    45361 atgtgaactt taagcgggtg aaagaagaca aagctgcgta atcaacgctg aggccagata
    45421 atactctctg gcctctctct tattagttat tctgattgcc aattaatatt atcaatttcc
    45481 gctaataaca atcccgcgat atagtctctg catcagatac ttaattcgga atatccaacg
    45541 tgttttctta ttactttcaa ggtcttgctc ttggggcagc ta
//
LOCUS       DAMZET010000015        45087 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.015, whole genome shotgun sequence.
ACCESSION   DAMZET010000015 DAMZET010000000
VERSION     DAMZET010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.015"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            1..227
                     /locus_tag="POB34_000977"
                     /pseudo
     CDS             1..227
                     /locus_tag="POB34_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            560..766
                     /locus_tag="POB34_000978"
     CDS             560..766
                     /locus_tag="POB34_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI5917969.1"
                     /translation="MYIPQNFLPRSRQKLLRSAPGQSFPAVAVMPLAAKVLKKVMVNT
                     GGNKRQVPAKRQNRGSRNSKDDGG"
     gene            792..1331
                     /locus_tag="POB34_000979"
     CDS             792..1331
                     /locus_tag="POB34_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI5917970.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGRAAGAQTQPEEGQQFSGQPQPEPQAEAGTKKGGAKTKGRGRKAAQPE
                     PQPQPPEGDDYGFSDDIPF"
     gene            1399..1632
                     /locus_tag="POB34_000980"
     CDS             1399..1632
                     /locus_tag="POB34_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HDI5917971.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            1660..1857
                     /locus_tag="POB34_000981"
                     /pseudo
     CDS             1660..1857
                     /locus_tag="POB34_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1912..2346
                     /gene="psiB"
                     /locus_tag="POB34_000982"
     CDS             1912..2346
                     /gene="psiB"
                     /locus_tag="POB34_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HDI5917972.1"
                     /translation="MKTELTLNVLQTMNTQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGSQA"
     gene            2343..3062
                     /locus_tag="POB34_000983"
     CDS             2343..3062
                     /locus_tag="POB34_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HDI5917973.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWYARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEAGMAARV
                     VGHAVREMERWMVPNKLREAA"
     gene            complement(3074..3262)
                     /locus_tag="POB34_000984"
     CDS             complement(3074..3262)
                     /locus_tag="POB34_000984"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein sok"
                     /protein_id="HDI5917974.1"
                     /translation="MKINLLRGYLLSATQHGNKPPSRHEAESLKRRAHHSPYTCTLTT
                     LTFPENNPLIQTVHGKSV"
     gene            3342..3500
                     /locus_tag="POB34_000985"
     CDS             3342..3500
                     /locus_tag="POB34_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDI5917975.1"
                     /translation="MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAA
                     FMAYESGK"
     gene            4421..4708
                     /locus_tag="POB34_000986"
     CDS             4421..4708
                     /locus_tag="POB34_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917976.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSQGVDKQFRHINIAEADHFKQFLRQIKRAGLDIRAIC"
     gene            4826..5647
                     /locus_tag="POB34_000987"
     CDS             4826..5647
                     /locus_tag="POB34_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDI5917977.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRIRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
     gene            complement(5944..6591)
                     /locus_tag="POB34_000988"
     CDS             complement(5944..6591)
                     /locus_tag="POB34_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HDI5917978.1"
                     /translation="MIQKTNQQKLVFAWGVVLLVVRTTNLLSLFVEWVKLFTDKVTRG
                     GKMKKWMLAICLMFINEICQATDCFDLAGRDYKIDPDLLRAISWQESRYRVNAIGINP
                     VTGYGSGLMQVDSQHFNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVTWEAV
                     GAYNAGFRKTERQNQRRLAYASDVYRIYTGIKSSKGIRLPATKKSLPEINSVQNN"
     gene            6868..7251
                     /locus_tag="POB34_000989"
     CDS             6868..7251
                     /locus_tag="POB34_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HDI5917979.1"
                     /translation="MARVNLYISNEVHEKINMIVEKRRQEGARDKDISLSGTASMLLE
                     LGLRVYDAQMERKESAFNQTEFNKLLLECAVKTQSTVAKILGIESLSPHVSGNPKFEY
                     ASMVDDIREKVSVEMDRFFPKNDGE"
     gene            7442..8128
                     /locus_tag="POB34_000990"
     CDS             7442..8128
                     /locus_tag="POB34_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDI5917980.1"
                     /translation="MCALDRRERPLNSQSVNKYILNVQNIYRNSPVPVCVRNKNRKIL
                     YANGAFIELFSREDKPLSGESYIRLQVEIFLSSLELECQALGHGSAFCRRFNFHGEIY
                     QIRMENVSFYNDESVVLWQINPFPDYPFFALNQSVSNTNTSGQLTIWNDLSPGTLVVF
                     SFYMLGVGHATIARELGITDRASEDRIKPVKRKIKEFFEHFDLFRVSCIYKGEIDSLL
                     SIIREFYNVK"
     gene            8222..8449
                     /gene="traY"
                     /locus_tag="POB34_000991"
     CDS             8222..8449
                     /gene="traY"
                     /locus_tag="POB34_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HDI5917981.1"
                     /translation="MRRRNARGGISRTVSVYLDEDTNNRLIKAKDRSGRSKTIEVQIR
                     LRDHLKRFPDFYNEEIFREVAEESESTFKEL"
     gene            8483..8842
                     /gene="traA"
                     /locus_tag="POB34_000992"
     CDS             8483..8842
                     /gene="traA"
                     /locus_tag="POB34_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HDI5917982.1"
                     /translation="MDAVLSVQGASAPVKKKSFFSKFTRLNMLRLARAVIPAAVLMMF
                     FPQLAMAAQGQDLMASGNTTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFLAG
                     FAIISVFIAVGMAVVGL"
     gene            8857..9168
                     /gene="traL"
                     /locus_tag="POB34_000993"
     CDS             8857..9168
                     /gene="traL"
                     /locus_tag="POB34_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HDI5917983.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            9190..9756
                     /gene="traE"
                     /locus_tag="POB34_000994"
     CDS             9190..9756
                     /gene="traE"
                     /locus_tag="POB34_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HDI5917984.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMAFNAPFAVSQNSADASYLQQMALSFIALRLNVSSETVDASHQALLQYIRP
                     SAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            9767..10471
                     /gene="traK"
                     /locus_tag="POB34_000995"
     CDS             9767..10471
                     /gene="traK"
                     /locus_tag="POB34_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HDI5917985.1"
                     /translation="MFGSLFFSCSVMAANGTLAPTVVPMVNGGQASIAISNTSPNLFT
                     VPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLSIQAVPREG
                     AGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQVPVTKETLQ
                     APAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMFSQPASQLL
                     AGARMDVYVIRDGEGN"
     gene            10471..11898
                     /locus_tag="POB34_000996"
     CDS             10471..11898
                     /locus_tag="POB34_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HDI5917986.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGTASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQSRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            11888..12460
                     /locus_tag="POB34_000997"
     CDS             11888..12460
                     /locus_tag="POB34_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009424415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HDI5917987.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVQEIDFVRQTAPAGQFPVRECLPSLHDLPGAEEKICRYRAADA
                     ADYVRETDRSLMQLVTAFWATLALMYMSFAAGTGKYPVRPGKMKCVRVATADERLKEV
                     LAEDTSLPGKIRECHVYFPDDRTIRNNGEK"
     gene            12457..12774
                     /locus_tag="POB34_000998"
     CDS             12457..12774
                     /locus_tag="POB34_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HDI5917988.1"
                     /translation="MNMRNINVITALSVPGKTVSDDFMHAVLSNCTTRIVLPAPKEFS
                     SESLPHNFNMAAVGVMKEGMSMRLFSSPGHIVVAGQSGGGKSVPVKELIDRIIQKYKE
                     PIR"
     gene            12771..13022
                     /gene="trbG"
                     /locus_tag="POB34_000999"
     CDS             12771..13022
                     /gene="trbG"
                     /locus_tag="POB34_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HDI5917989.1"
                     /translation="MNKLVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVA
                     YVYKSSRNTVFENPDIVKTCTVRDLKNDFVNCDEKGEGQ"
     gene            13019..13534
                     /gene="traV"
                     /locus_tag="POB34_001000"
     CDS             13019..13534
                     /gene="traV"
                     /locus_tag="POB34_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HDI5917990.1"
                     /translation="MKQISFFIPLLGTLLLSGCAGTSTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            13646..13825
                     /gene="traR"
                     /locus_tag="POB34_001001"
     CDS             13646..13825
                     /gene="traR"
                     /locus_tag="POB34_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HDI5917991.1"
                     /translation="MTMTGINRIRQKINVHGIPVYLCEACGNPIPDARRKIFPGVTLC
                     VECQAYQERQRKHYA"
     gene            13818..14291
                     /locus_tag="POB34_001002"
     CDS             13818..14291
                     /locus_tag="POB34_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917992.1"
                     /translation="MHKSETEKIDSILDEHQQIYRRHNRIDNILAFFACIAWIPCIIA
                     AFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQREQDTIRKFTEEKKSE"
     gene            14371..14586
                     /locus_tag="POB34_001003"
     CDS             14371..14586
                     /locus_tag="POB34_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917993.1"
                     /translation="MHTFIFLVLIKNINGGGSWNVTPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPLNNVMDTLNGGQ"
     gene            14614..14976
                     /locus_tag="POB34_001004"
     CDS             14614..14976
                     /locus_tag="POB34_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5917994.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMLICGLFYNDYLEQQPATPEQLTEI
                     IQDTPCAAEAFKEAIKSDTSDYQPEPLSLGKAKKLASACRERNEMAEVKRVRENERNK
                     IREKQLQALNDAHSAKEH"
     gene            15102..17732
                     /gene="traC"
                     /locus_tag="POB34_001005"
     CDS             15102..17732
                     /gene="traC"
                     /locus_tag="POB34_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HDI5917995.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGVPFCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMNAAATQFPLPEGMNLPLTLRHYR
                     VFFSYCSPSKKKSRADILEMENLVKIIRASLQGASITTQAVDAQAFIDIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNARDNDQYVESPTIRSRLDEMI
                     VLLDQYTAGGTYGRYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            17729..18115
                     /gene="trbI"
                     /locus_tag="POB34_001006"
     CDS             17729..18115
                     /gene="trbI"
                     /locus_tag="POB34_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HDI5917996.1"
                     /translation="MTTTQKTTDVTAPQRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            18112..18744
                     /gene="traW"
                     /locus_tag="POB34_001007"
     CDS             18112..18744
                     /gene="traW"
                     /locus_tag="POB34_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HDI5917997.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALELSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            18741..19733
                     /locus_tag="POB34_001008"
     CDS             18741..19733
                     /locus_tag="POB34_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HDI5917998.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPIDALFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVTRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            19742..20380
                     /gene="trbC"
                     /locus_tag="POB34_001009"
     CDS             19742..20380
                     /gene="trbC"
                     /locus_tag="POB34_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HDI5917999.1"
                     /translation="MKLSMKSLAALLMMLNGAVMASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            20377..22185
                     /gene="traN"
                     /locus_tag="POB34_001010"
     CDS             20377..22185
                     /gene="traN"
                     /locus_tag="POB34_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HDI5918000.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFAHQIKGQGSSSIQG
                     FKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQAGRDVVNRADSIVGNTGQQCSAQEISRSEYTNYTCERDLQVEQYCTRT
                     ARMELQGSTTWETRTLEYEMSQLPAREVNGQYVVSITSPVTGEIVDAHYSWSRTYLQK
                     SVPMTITVLGTPLSWNAKYSADASFTPVQKTLTAGVAFTSSHPVRVGNTKFKRHTAMK
                     LRLVVRVKKASYTPYVVWSESCPFSKELGKLTKTECTEAGGNRTLVKDGQSYSMYQNC
                     WAYRDTYVTQSADKGTCQTYTDNPACTLVSHQCAFYSEEGACLHEYATYSCESKTSGK
                     VMVCGGDVFCLDGECDKAQSGKSNDFAEAVSQLAALAAAGKDVAALNGVDVRAFTGQA
                     KFCKKAAAGYSNCCKDSGWGQDIGLAKCSSDEKALAKAKSNKLTVSVGEFCSKKVLGV
                     CLEKKRSYCQFDSKLAQIVQQQGRNGQLRISFGSAKHPDCRGITVDELQKIQFDRLDF
                     TNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            22212..22469
                     /gene="trbE"
                     /locus_tag="POB34_001011"
     CDS             22212..22469
                     /gene="trbE"
                     /locus_tag="POB34_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HDI5918001.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAIVISPVIIWSWDTVKETTAGEML
                     AACFVIFYSGVLLFILYFCFSALTDLQKSDERKSDERNEDE"
     gene            22462..23205
                     /gene="traF"
                     /locus_tag="POB34_001012"
     CDS             22462..23205
                     /gene="traF"
                     /locus_tag="POB34_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HDI5918002.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAV
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            23221..23568
                     /gene="trbA"
                     /locus_tag="POB34_001013"
     CDS             23221..23568
                     /gene="trbA"
                     /locus_tag="POB34_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HDI5918003.1"
                     /translation="MSEDCLKMFTGVVLLIFVIIAGYFFSERNDRKMFLLSSLVFLVI
                     NIACLYVLTARFWFLCGAIMNQGAAMVVPMVIGSLPNVTSFDGFRRIFICIMLSSVWS
                     GVMWFFIRGLMTG"
     gene            23687..23971
                     /gene="traQ"
                     /locus_tag="POB34_001014"
     CDS             23687..23971
                     /gene="traQ"
                     /locus_tag="POB34_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HDI5918004.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSREEREALEARQQAMMEGQQEGRHVSH"
     gene            23958..24503
                     /gene="trbB"
                     /locus_tag="POB34_001015"
     CDS             23958..24503
                     /gene="trbB"
                     /locus_tag="POB34_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HDI5918005.1"
                     /translation="MSLIKSLLFTLLLAAAAVQASTRDEIERLWNPQGMAAQPAQPAA
                     GTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     AGFMARMDTVLQMYGGKKGAK"
     gene            24433..24795
                     /gene="trbJ"
                     /locus_tag="POB34_001016"
     CDS             24433..24795
                     /gene="trbJ"
                     /locus_tag="POB34_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009424419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HDI5918006.1"
                     /translation="MLPVLWRGWIPFCRCTEGKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIHQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRRTGRKYRD
                     IPAEMSYAPGEHIKGGQE"
     gene            24795..26168
                     /locus_tag="POB34_001017"
     CDS             24795..26168
                     /locus_tag="POB34_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HDI5918007.1"
                     /translation="MPRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNVQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQAAQGIIGG
                     LFPRTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGKSDSVRDKASDKDKERVTKNI
                     NIMWNALSKNRMFDGNKELKEFVMTLTGSLVFGPNGEITPLSARTTDRSIIRAMMEGG
                     TAKIYHCNDSDKCLKVVADTPVTISRDNALKSQITKLLASIQNKAVSDTPLDDKEKGF
                     ISSTTIPVFKYLVDPQMLGVSNSMIYQLTDYIGYDILLQYIQELIQQARAMVATGNYD
                     EAVIEHINDNMNDATRQIAAFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            26165..28987
                     /gene="traG"
                     /locus_tag="POB34_001018"
     CDS             26165..28987
                     /gene="traG"
                     /locus_tag="POB34_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HDI5918008.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSVTYSKTGMLFGAELVSKSTDFLSRNPEIANLFQDY
                     VQNCVMGDIYLNHKYTLEELMASADPYTLIFSRPSPLRGVYDSNNNFVTCKDASVSLK
                     DKLNLDTQSGGKTWHYYAQQLFGGRPDPNLLFSTLIGDSYSYFYGSSKSASQIIRQNV
                     TINALKEGITSYAARNGDSASLVNLATTSSMEKQRLAHVSIGHVAMRTLPMTQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQSWPMLYAILNSAMTFYAKQNGAPV
                     VLSEISQIQLKYSDLASTAGYLSMMIPPLSWMMVRGLGAGFSSVYSHFASSAISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNSLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATRELASRSTRGSAGMY
                     GDAHAEWGVKPKILGVGGGVGVRGGGRASIDWSDEDAHQASSGSRASHDARHDIDARA
                     TQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNTWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYRGNIGDTQSSIRGEENIVGRQYSELQNHHKTEALSQNNKYNEEKSG
                     QERMPGADSPQELMKRAKEYQDKHKQ"
     gene            29002..29505
                     /locus_tag="POB34_001019"
     CDS             29002..29505
                     /locus_tag="POB34_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HDI5918009.1"
                     /translation="MIMKNLAHIALVTVIQFIACYLADWGSAETVFILCFIVLWQGLF
                     IWLFSQIRKKRHVSDEFKFSKGVWYILMPVSSLLSPLLSLMVFIIGTLYELRRISGCV
                     SVREWMQSQVNEQTNEDLHLDFDDVNFDYVDFYRTNPATGLAMHGGFDSAGNTFGTCW
                     QDYDDRR"
     gene            29537..30268
                     /locus_tag="POB34_001020"
     CDS             29537..30268
                     /locus_tag="POB34_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HDI5918010.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSSSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            30521..32710
                     /gene="traD"
                     /locus_tag="POB34_001021"
     CDS             30521..32710
                     /gene="traD"
                     /locus_tag="POB34_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HDI5918011.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDAKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PQQPQQPQQPQQPVSPAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESG
                     EVVDMAAYEAWQQENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            32710..37980
                     /gene="traI"
                     /locus_tag="POB34_001022"
     CDS             32710..37980
                     /gene="traI"
                     /locus_tag="POB34_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HDI5918012.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGINVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVTSV
                     SEDAMTVVVPGRAEPASLPVADSPFTALKLENGWVETPGHSVSDSAKVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KTGLHTPAQQAIHLALPVVESKNLAFSMVDLLTEAKSFAAEGTSFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLSASERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISRDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVRDIAGLERDRSAISEREAALPESVLREPQRVREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            38000..38746
                     /gene="traX"
                     /locus_tag="POB34_001023"
     CDS             38000..38746
                     /gene="traX"
                     /locus_tag="POB34_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HDI5918013.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQSWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            38805..39665
                     /locus_tag="POB34_001024"
     CDS             38805..39665
                     /locus_tag="POB34_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI5918014.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLIVIAGQDSVNPPEQGKALYDAVASGTKELYEEADACHYDIYEGAFFERVAA
                     VQTQWFKKHL"
     gene            39768..40328
                     /locus_tag="POB34_001025"
     CDS             39768..40328
                     /locus_tag="POB34_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HDI5918015.1"
                     /translation="MTEQKRPVLTLKRKTEGETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELTAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEV
                     YAAERLDKIRRQNRIKAELQAVLDEQ"
     gene            40457..40669
                     /locus_tag="POB34_001026"
     CDS             40457..40669
                     /locus_tag="POB34_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HDI5918016.1"
                     /translation="MNGTGRDSQNSGVWRYRQVGRRAVRLEVRGDWPGAAEAWLRAAQ
                     SAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            40914..41375
                     /locus_tag="POB34_001027"
     CDS             40914..41375
                     /locus_tag="POB34_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDI5918017.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLINIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            41421..41630
                     /gene="hha"
                     /locus_tag="POB34_001028"
     CDS             41421..41630
                     /gene="hha"
                     /locus_tag="POB34_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HDI5918018.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLLNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            41668..42258
                     /locus_tag="POB34_001029"
     CDS             41668..42258
                     /locus_tag="POB34_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HDI5918019.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGAAQQSPEHRS"
     gene            42498..42755
                     /gene="repA"
                     /locus_tag="POB34_001030"
     CDS             42498..42755
                     /gene="repA"
                     /locus_tag="POB34_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HDI5918020.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGKPLSEAERQRAASARKRSVCKEI
                     KVFVRPELKNYLTSLCAEEGVTQAELIERLIEKEACHRNMM"
     gene            42991..43065
                     /gene="tap"
                     /locus_tag="POB34_001031"
     CDS             42991..43065
                     /gene="tap"
                     /locus_tag="POB34_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HDI5918021.1"
                     /translation="MPGKVQDFFLCSLLLCNISAGWCD"
     gene            43058..43915
                     /gene="repA"
                     /locus_tag="POB34_001032"
     CDS             43058..43915
                     /gene="repA"
                     /locus_tag="POB34_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HDI5918022.1"
                     /translation="MTDLHQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEVAVASARRSRVEWENRQRKKQGLDALGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDAGRERQDIVTLVKRQLTREISEGRFTANGETVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            44618..44776
                     /locus_tag="POB34_001033"
     CDS             44618..44776
                     /locus_tag="POB34_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001700046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918023.1"
                     /translation="MAENGYGLAGLGMGKVKSINQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGISW"
     gene            44831..>45087
                     /locus_tag="POB34_001034"
     CDS             44831..>45087
                     /locus_tag="POB34_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918024.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVAAGRQQLGRGDVLPHDMVESSAAAWRDEMSRKV"
BASE COUNT        11205 a        10724 c        12741 g        10417 t
ORIGIN      
        1 cacttgtatt gtcgatctga ttatcagcgt gagactacga ttccattaat gcctgccagt
       61 gagtgtgtgg aacatatctt ggtcagtggc ttggtctgtg atgtgctgcg tataccgggt
      121 gaaccgcccc gccgcttgtt tgaccgctat attctgcggg agttttcttg gaggcacggg
      181 atgaaatccg cagcatggcg gatgccctgg caaaaatccg gaaatgatga cgatggcggg
      241 gaaaatcccc gcctttttcc tgccggtccc cggacagagc acaaaggaag tcgccgccgg
      301 ctccgcttta cccggccatg cggagcatgg ccttgtgggg gttcagttat gtggacgtcg
      361 agtcgtgtgc gggctgtgcc gtgcctccat cttagccggg ctggcgttga tgcaaggggc
      421 aacgccgttt cgcaaaccgc gcttcacgtc gttgcggcgg gtccctgccc gttgttctgt
      481 ccggtgccat tcacctgttt aagcccctgt cgccgggggc tgatacagcc acaaccgtct
      541 ccgccagtcc aggggtgaaa tgtacatccc gcaaaacttt ttgcccagaa gcaggcaaaa
      601 acttttgcgg tctgcccccg gacagtcgtt tccggccgtg gcagtgatgc cccttgcggc
      661 gaaggttctc aaaaaggtga tggtgaatac cggagggaac aaaaggcagg tccccgcaaa
      721 acgtcaaaac cgcggctccc gaaactcaaa agatgatggg ggctgaaaac atcaacagaa
      781 ggaggcacat catggcagtt cgtggcatta acaaggtcat tctcgtggga cgcctgggaa
      841 aagatccgga agtccgttac atccccaacg ggggcgcagt ggcaaacctg caggtcgcca
      901 cgtcggaaac ctggcgtgac aaacagacag gggagatgcg ggagcagaca gaatggcatc
      961 gcgtggtgct gttcggcaag ctcgcggaag tggctggcga atatctgcgc aagggcgcgc
     1021 aggtctacat cgaaggtcag cttcgcaccc gtagctggga ggataacggt attacccgtt
     1081 acgtcaccga aattcttgtt aagaccacgg gcaccatgca gatgctggga cgtgccgcag
     1141 gtgctcagac tcagccggaa gaggggcaac agttcagcgg tcagcctcag ccggaaccac
     1201 aggcggaggc cggtacgaaa aaaggtggcg caaaaacgaa aggccgtgga cgtaaggccg
     1261 cgcagccgga gcctcagccg caaccgccgg agggtgacga ttacgggttt tcagacgata
     1321 tcccgttctg atcgggctga ctgtgataac cgccccgccc cgccccgttc tgtgcggggc
     1381 atcaccggag agatgaggat gagcgaatat ttcagaatac ttcaggggct gcctgacggt
     1441 ccttttaccc gcaaacatgc cgaagctgtt gccgcacagt accggaacgt ctttatcgag
     1501 gatgaccacg gcgaacagtt tcgcctggtt gtccgtaata acggtgcaat ggtctggcgt
     1561 acctggaatt ttgaggacgg tgccgggtac tggatgaacc atgtcatcag ggatttcggg
     1621 attcttaagt aagagaggtg cccgacgcgc gaacgtccgc cgcagattat cgcagccctg
     1681 aataaggcag ggctgtccgg tgccgcacgg gacgcggaga agatgaagaa aggtgatgcg
     1741 gctgaacatg cagagcacca tatgaaagac aaccgctggg ttccaggctg gatgtgtgca
     1801 ccacgcccac agacggatgc cactgaacgc accgataacc tggctgatgc cgcctgatga
     1861 acaaccacac cgccccgccg gagacggggc ggcagcaagg gagataccgt gatgaaaact
     1921 gaactgaccc tgaatgtatt acagaccatg aacacacagg aatatgaaga tatccgggct
     1981 gccggaagtg atgaacgccg tgagctgact cacgccgtga tgcgggagct ggatgcgccg
     2041 gataactgga cgatgaacgg cgagtacggc agcgagtttg gcggcttttt ccccgtccag
     2101 gtccgtttca cgcctgccca cgaacgtttt cacctggcgt tatgttcacc gggcgatgtc
     2161 tctcaggtct gggtgctggt tctggtgaat gccggtggtg agccgtttgc ggtggttcag
     2221 gtacagcggc ggtttgcacc ggaagctgtc agccattcac tggcactggc ggcgtcactg
     2281 gacgcgcagg ggtacagcgt cagtgacatc atccatatcc tgatggcgga aggaagtcag
     2341 gcatgagcgc acgttcacag gcactggttc ctctcagcac agagcaacag gccgcatggc
     2401 gggcggtggc ggagaccgaa aaacgccgcc atcagggtaa cacgctggct gaatatccgt
     2461 atgcgggggc gtttttccgc tgtctgaacg gaagccgcag gatatcgctg tctgacctgc
     2521 gatttttcat gccgtccctg accgcagaag aactgcacgg caaccgtctt cagtggctgt
     2581 atgccatcga tgtgctgatt gaaacacagg gcgaggtttg cctccttccc cttccaggcg
     2641 atgcagcaga acgcttgttc ccgtcggtgc ggtttcgtgt cagggaacgc agtcgccata
     2701 aatccgcgct ggtcatgcag aagtacagcc gccagcaggc gagggaggca gagcagaaag
     2761 cccgtgcgta ccaggcgctg gtcgctcagg ctgaaatcga actggcattc cattcgccgg
     2821 aaacagtcgg aagctggtat gcacgctgga gtgaccgggt ggcagagcat gacctggaaa
     2881 ccctgttctg gcagtggggc gagcgttttc cgtcactggc cggaatggaa cgctggcagt
     2941 ggcaggatat gccattctgg caggtcatcg cagaggctgg tatggcggca agggtggtcg
     3001 gtcatgctgt tcgtgagatg gagcggtgga tggtgccgaa caaactccgg gaggcagcgt
     3061 gatgcggcaa caatcacacg gatttcccgt gaacggtctg aatgagcgga ttattttcag
     3121 ggaaagtgag tgtggtcagc gtgcaggtat atgggctatg atgtgcccgg cgcttgaggc
     3181 tttctgcctc atgacgtgaa ggtggtttgt tgccgtgttg tgtggcagaa agaagatagc
     3241 cccgtagtaa gttaattttc attaaccacc acgaggcatc cctatgtcta gtccacatca
     3301 ggatagcctc ttaccgcgct ttgcgcaagg agaagaaggc catgaaacta ccacgaagtt
     3361 cccttgtctg gtgtgtgttg atcgtgtgtc tcacactgtt gatattcact tatctgacac
     3421 gaaaatcgct gtgcgagatt cgttacagag acggacacag ggaggtggcg gctttcatgg
     3481 cttacgaatc cggtaagtag caacctggag gcgggcgcag gcccgccttt tcaggactga
     3541 tgctggtctg actactgaag cgcctttata aaggggctgc tggttcgccg gtagcccctt
     3601 tctccttgct gatgttgtac gggcatgaac aacctgactt caggaaggtc gtttcccttc
     3661 aggaacgggg atgaacgcgc gcctgcggcg cccgttcttt tcccccgcct tctctggtta
     3721 tggcctgtca gaaatcacat caccttccct gtgattattc tctttttctt cctgctctga
     3781 ttctgactac tgcagtgttg tcctgtctgc cgggcgtacc ttcggctccc ggctgcctgg
     3841 cggcatccgg tcgtcaggcc cgactccgac aggcagctgc tggcgcacct gccagtctcc
     3901 gccgaacccg ctggcgcggt ttcgggctgt cgctacggtc cgatgcgtga aaacccctgg
     3961 cttgcagcga ccgttccggt cgcgccgcct ctgtcgcgcc gtgacctgcg ctcccggcat
     4021 cccggcaaaa ggcgccggga tgtaaggcgc aacgccgttt cgcaaaccgc gcttcacgtc
     4081 gttgcggcgg gtccctgccc gttgttgtgt ccggtgccat tcaccagttt aagcccctgt
     4141 cgccgggggc atagacagcc acaaccgtct ccgccagtcc aggggtgaaa tgcacatccc
     4201 gcaaaacttt ttgcccagaa gcaggcaaaa acttttgcgg tctgcccccg gacagtcgtt
     4261 tccggccgtg gcagtgatgc cccatgcggc gaaggttctc aaacaggtga tggtgaatac
     4321 cggagggaac aaaaggcagg tccccgcaaa acgtcaaaac cgcggctccc gaaactcaac
     4381 agatgatggg ggctgaaaac cagagagagg agaaagcagg atgagtaccc gaaatatcca
     4441 cgttaacacc gcgtcgtaca cgcttcttgt tgccgggaag aaaaaaaaca ccggggaaga
     4501 gtgggacgtc ctggaattca gcagcctgac tgagctgaaa aaatatcgca aaagccaccc
     4561 ggaaaagatg gccttcagtt atagctacgc gctcagtcag ggtgtggata agcagttccg
     4621 ccatatcaac attgcggaag ccgatcactt caaacagttc ctgcgtcaga ttaagcgtgc
     4681 cggcctcgat atccgggcga tctgctgacc ggatgtgatg aatatccctc ctgattgttg
     4741 caatcttgtt aatggagatc ggggggatga tattaagcgg aaaaattttt tattaaattt
     4801 attctttcat aaaggagtag ctgttatgcg attagcttcc cgttttggtc ggtataattc
     4861 catccgccgt gaacgtcctt taacggatga tgaattaatg cagttcgtgc cttcggtatt
     4921 ttccggtgat aaacatgagt cccggagtga acgttatacg tatattccaa caatcaatat
     4981 catcaataag ttacgtgatg aaggtttcca gccattcttt gcctgtcaga gtcggattcg
     5041 tgatttggga cgtcgcgaat acagtaaaca tatgttacgt cttcgcaggg aagggcatat
     5101 taacggacag gaagttcctg aaattatcct gcttaattca catgatggtt catccagtta
     5161 tcagatgatc cccggaattt ttcgttttgt ctgcacaaat ggcctggtgt gcggaaataa
     5221 ttttggcgaa atccgcgttc cacataaagg tgatattgtc gggcaggtta tcgagggagc
     5281 gtatgaagtg ctcggtgtct ttgataaggt cactgataat atggaggcga tgaaagaaat
     5341 tcatcttaac agtgacgagc aacatttatt tggcagagct gcactgatgg tcaggtatga
     5401 agatgaaaat aaaacgccag tgacacctga acagataatt actccccgtc gtcgggaaga
     5461 caaacagaac gatctctgga caacctggca gcgggttcag gagaatatga taaaaggtgg
     5521 attatcgggg cgaagtgcct ccgggaaaaa taccaggaca agagccatta caggtattga
     5581 tggtgatata agaatcaaca aggcgttatg ggtgattgcc gaacagttca gaaagtggaa
     5641 gtcatgataa tataagtaaa gccccgaaaa aattttcggg gctttactta tattatcatt
     5701 tttcggcatt gctgtgtcgt ctgatatacc tcaatttcat caaccggatt tccggttgtc
     5761 tgtaaatatt gtgcccgcga aaaatggtct ggcaggaggc tgcacagtta ccgggtatat
     5821 ataagatggg ttcccgcttc gcggggcttc ggccagtcag tattgtcact catatgatat
     5881 ttttgtgtgt ggccttccat gccgcttttg cggcataacc ggtatctgac aatgtctgta
     5941 agattaattg ttctgcacgc tgttaatttc cggaagtgat ttcttcgtgg ccggaagccg
     6001 gataccttta ctgcttttta tcccagtata aatccggtaa acatctgatg cgtaggcaag
     6061 acgtctctgg ttctggcgtt cggttttcct gaatccggca ttgtatgcac caacggcctc
     6121 ccaggtgacg ccccattttt taaatgctat tgccagataa taagcaccgg tataaatgtt
     6181 catgcaggga tctgttgtca gatgttctgg cttaattccg tagcgggcca gttcgttaaa
     6241 atgctgggaa tctacctgca tcagtccgct gccatatccc gttaccggat taataccgat
     6301 ggcattaacc cggtaacggg attcttgcca tgatattgct ctcagtaaat caggatctat
     6361 tttgtaatcc cggcctgcaa gatcaaagca atcagtggcc tggcagatct catttataaa
     6421 catcaggcag atggctaaca tccatttttt catttttcca cctctggtga ctttatccgt
     6481 aaataattta acccactcca caaaaaggct caacaggttg gtggttctca ccaccaaaag
     6541 caccacaccc cacgcaaaaa caagtttttg ctgatttgtt ttttgaatca ttagcttatg
     6601 ttataaataa tgtattttaa tttattttac attattaaat agggtcgttg acgaccctat
     6661 ggcgacccta tgacgaccct tgttagggtc agtactgaca attttcatga aaaacatttg
     6721 gatatattag ggccgcccct agcagcgccg tgcgtttttt tatagtatgc ccctaggggc
     6781 gctgctaggg gtgctcttct tttttcagac catactatga gtagatgaaa taaaacactt
     6841 atttcagaat ctaaaaggta ctgtcttatg gccagagtaa atctgtatat cagtaatgag
     6901 gttcatgaaa aaattaacat gattgttgaa aagcgtcgtc aggagggagc aagagataaa
     6961 gatataagcc tttcaggaac tgcttcaatg cttcttgaat tagggcttcg tgtatatgac
     7021 gcccagatgg agcgtaaaga gtctgcgttt aaccagacag agtttaataa acttcttctt
     7081 gaatgtgctg taaaaacaca gtcaacggta gcaaagattt taggtattga gtctctcagc
     7141 cctcatgtct ccggaaaccc gaagtttgaa tatgccagta tggttgacga tatcagagag
     7201 aaagtgtctg ttgagatgga ccggtttttt ccaaaaaatg atggtgaata aatgaaaatg
     7261 aagtttgagt tggttcaaat ataagaattt ttatgattca aaaagatgac aatacgtcag
     7321 ataattagta tattaattac gtggttaata ccacgttaaa atttgaattt gaaaatcgcc
     7381 gatgcaggga ggtctgaact ccctgcatcg actgtccata gaatccttaa cggaggttcc
     7441 tatgtgtgcg ctggatcgta gagaaaggcc acttaacagt caatctgtaa ataaatacat
     7501 ccttaacgtt cagaatatct acagaaattc tcccgttccg gtttgtgtcc gtaacaaaaa
     7561 ccggaaaatc ctttatgcca atggggcttt tattgaactc ttttccagag aagataaacc
     7621 tttatccgga gagagttata tacgtctgca ggttgaaatt tttctttcat cacttgaact
     7681 ggaatgccag gctcttggac atggctctgc attttgtcgt cgttttaatt ttcatggcga
     7741 aatctatcag ataaggatgg agaatgtttc tttttataat gacgaatctg ttgttttatg
     7801 gcaaattaat ccgtttcctg attatccatt ttttgcgtta aatcagagtg taagtaatac
     7861 aaatacttct ggtcaattaa cgatatggaa tgatctttct ccaggaacat tggttgtttt
     7921 ctctttttat atgctgggtg ttggtcacgc aacaattgcc agagaattgg gtattacaga
     7981 cagagcatct gaggatcgaa ttaaaccagt taaacggaaa ataaaagagt tttttgaaca
     8041 ctttgattta ttcagagtgt catgtatcta taagggggaa atagattcgc tattaagtat
     8101 aattcgtgaa ttttataatg ttaaataaat gttaaataag acacgccagt tttggcgtgt
     8161 tttttttcgc cggtttctgt gcaatcataa ttttattagt cgggaaacag aggtgaattt
     8221 agtgaggagg cgtaacgcga gaggcggtat aagcagaacg gtctcggtgt atctcgatga
     8281 agatacaaac aaccgactta ttaaggccaa agatcgtagt ggaagaagta aaacgataga
     8341 agtccagatc cgtctcagag atcatctgaa acggtttcct gatttctata acgaagaaat
     8401 attcagggag gtggccgaag agagcgagtc aacatttaaa gagctttagt ttttatctat
     8461 caatattaaa cagaggaatc atatggacgc cgttttaagt gttcagggtg cttctgcgcc
     8521 cgtcaaaaag aagtcatttt tttctaaatt cactcgtctg aatatgcttc gcctggctcg
     8581 cgctgtgatc ccggctgctg ttctgatgat gttcttcccg caactggcaa tggcagcaca
     8641 gggccaggac ttgatggcaa gcggtaacac cacggttaag gcgaccttcg gtaaggactc
     8701 cagtgttgtt aaatgggttg ttctggctga agttctggtc ggtgcagtca tgtacatgat
     8761 gaccaaaaac gtcaagttcc tggccggttt tgccatcatc tctgtattta ttgctgtggg
     8821 tatggccgtc gttggcctct gacaggaaat aaaacgatgt cgggagacga gaataaactt
     8881 aagaaatatc gtttcccgga aacactgacc aaccagagcc gctggtttgg cctgccactg
     8941 gatgaactga tccccgcagc aatctgtatt ggctggggta tcacaacatc gaaatatctg
     9001 ttcggtattg gtgcagcggt tctggtttat ttcgggatta aaaaactgaa aaaagggcgg
     9061 ggcagttcct ggttacgtga cctgatttac tggtatatgc caacagccct gctgcgcggt
     9121 atttttcata atgttcccga ttcgtgtttc cggcagtgga ttaaatagaa ctgataccag
     9181 gattgttata tggaacacgg tgcccgttta agtaccagtc gtgtaatggc catcgccttt
     9241 atatttatgt cagtgcttat tgttctcagc ctctctgtta acgtcattca gggggtgaat
     9301 aactaccgtc ttcagaatga gcaacgcact gccgtgacgc caatggcatt taatgccccc
     9361 tttgccgtgt cacagaacag tgccgacgcc tcttatttac agcagatggc gctgtcattt
     9421 attgccctcc gtctgaatgt ttcatcagaa accgtcgatg cctcacatca ggcgcttctg
     9481 caatatatcc gcccgagcgc acagaaccag atgaaagtta ttctggctga agaagcgaag
     9541 cgtattaaaa acgataacgt gaactcagcc tttttccaga ccagtgttcg tgtctggcct
     9601 cagtatggcc gtgtggaaat tcgaggtgtg cttaaaacct ggattggtga ttcaaaacct
     9661 ttcactgata tcaaacatta catccttatt ctgaagcggg aaaacggggt gacctggctg
     9721 gataatttcg gggaaacaga cgatgagaaa aaataatacg gcaataatat tcggcagcct
     9781 gtttttttcc tgcagcgtga tggccgcaaa cggtacgctg gcccccaccg tggtgccaat
     9841 ggtgaacggt ggtcaggcca gtattgccat cagcaatacc agcccgaatc tgtttaccgt
     9901 tcccggtgac cggattatcg ccgtgaacag tctggatggt gccctgacca ataatgagca
     9961 gaccgcctcc ggcggtgtgg tggttgccac cgtcaacaaa aagcccttta cgttcattct
    10021 ggaaacagaa cgtggtctga atctttccat tcaggccgtt ccccgtgaag gcgcggggcg
    10081 taccattcag ctggtcagtg acctgcgcgg aaccggagaa gaagccggtg cgtgggaaac
    10141 gtccacgcct tacgaatccc tgcttgtgac catcagccag gccgtccgtg gcggaaaatt
    10201 acccgcaggc tggtatcagg tcccagtgac aaaggaaacc ctgcaggccc cggcggggct
    10261 gtcttcagtg gcagatgccg tatggacggg gaatcacctg aagatggtcc gctttgccgt
    10321 ggaaaataaa acgctgtctg ccctgaatat ccgggaaagt gacttctggc agccgggaac
    10381 ccgtgccgtg atgttcagcc agcctgccag ccagttactg gcaggtgcgc gcatggatgt
    10441 gtatgtcatc cgtgacgggg agggcaactg atggccagta tcaataccat tgtgaaacgc
    10501 aagcagtacc tgtggctggg gattgtggtt gtcggtacag cctccgcgat tggtggggca
    10561 ctgtatctgt ctgatgtgga catgtccggt aacggtgaaa ccgtggctga acaggagcct
    10621 gtgccggata tgaccggtgt ggtggatacg acctttgatg acaaggtgcg tcagcatgcc
    10681 accacagaga tgcaggtgac ggcagcgcag atgcagaagc agtatgagga aatccgtcgt
    10741 gagctggatg ttctgaacaa acagcgcggt gatgaccagc gtcgtattga aaagctggga
    10801 caggacaatg ccgccttggc agagcaggta aaagccctgg gtgctaatcc cgtcacggcg
    10861 accggtgagc ctgtaccgca gatgcctgcc tcaccgcccg gcccggaagg cgaaccacag
    10921 ccaggaaaca cccccgtatc cttcccgccg cagggcagcg ttgctgttcc accgccgacg
    10981 gcgttttatc ccgggaatgg tgtcacgcca ccaccacagg tgacgtacca gtctgtgccg
    11041 gtgcctaacc ggatacagcg taaggtgttt acccgtaatg agggaaaaca gggaccatca
    11101 ctgccgtaca ttccgtcagg aagttttgcg aaagccatgc tgattgaagg ggcggatgcc
    11161 aatgcctctg tcaccggtaa tgaatccacg gtgccgatgc agctgcgtat caccggcctg
    11221 gtggaaatgc cgaacagcaa gacgtatgac gcaacgggat gttttgtggg tctggaagcc
    11281 tggggggatg tgtccagtga gcgtgccatt gtacgcaccc gcaatatcag ttgcctgaag
    11341 gacggcaaaa ctattgatat gccgattaag gggcatgtca gcttccgggg taaaaacggt
    11401 atcaagggcg aagtggtgat gcgtaacggc aaaatcctcg gctgggcatg gggcgcggga
    11461 tttgttgacg gtatcggtca gggaatggag cgcgcctccc agccggctgt cgggctgggt
    11521 gccacagccg cttacggggc tggtgatgtc ctgaaaatgg gtatcggtgg cggcgcatcg
    11581 aaagccgcac agacgctcag tgactactac atcaaacgtg ccgaacagta tcacccggtg
    11641 ataccgattg gtgcgggcaa cgaagtgacc gtggtgttcc aggacggctt ccagctgaaa
    11701 accgtggaag agatggcgct ggaacgcacg cagagcagag cggaagaaga caatccggaa
    11761 agtccggttc ctgttccgcc atcggctgaa agtcatctta acggctttaa tactgaccag
    11821 atgctgaagc agctgggcaa cctgaatccg cagcagttta tgtccggcag ccagggaggg
    11881 ggcaacgatg gcaaataata tgtcgtcccg tcaggcgtgc catgccgccc ggtatgttgt
    11941 cgcccgggtt ctgcgaggac tgttctggtg tctgaagtac accgtcattc tgccactggc
    12001 cacaatggcc ctgatggctc tgtttgtttt atggaaggac aacaccacac caggaaaatt
    12061 actggttcag gaaattgact tcgtaagaca aacagcccct gccgggcagt ttcctgtaag
    12121 agaatgtttg ccttctctgc atgatcttcc aggagcagaa gagaaaatct gccgttaccg
    12181 tgctgctgat gctgcagatt atgtcaggga gactgaccgg tcactgatgc aactggtcac
    12241 cgcattctgg gcaacgctgg cacttatgta tatgtcgttt gcggcaggca ccgggaaata
    12301 tccggtgcga cccggaaaaa tgaaatgtgt ccgtgtggca acagcagatg agcgtctgaa
    12361 ggaagttctt gcagaagaca cttccctgcc gggaaaaatc agggaatgcc atgtttattt
    12421 ccccgatgac agaacaatca gaaacaacgg agaaaaatga acatgcgtaa tattaatgtt
    12481 attacagccc tttctgtgcc gggtaaaacc gtgtcagatg attttatgca tgctgttctc
    12541 agtaactgca ccacaagaat cgtccttcct gcaccgaagg aattcagttc tgaatcattg
    12601 ccgcacaatt ttaatatggc tgctgtcggt gtgatgaagg agggaatgtc gatgcgttta
    12661 ttttcttctc cggggcatat tgtggttgcc ggacaatccg gtggcggaaa atctgttccg
    12721 gtgaaagaac taatcgacag aattattcag aaatataagg aacccattcg atgaataagc
    12781 tggtttctga tggcagcgtc aaaaaaataa attatccggt gctgtatgag tccggaataa
    12841 cacctccgtt atgtgaagtc agtgcaccag agcctgatgc gggtgggaaa cgcattgtgg
    12901 catatgttta taaatcatca cggaatacgg tttttgaaaa tcctgacatt gtaaaaacct
    12961 gtacggtcag ggatttgaag aatgattttg tgaactgcga tgagaaaggt gagggacaat
    13021 gaaacagatt tctttcttta ttcctctgct ggggacctta ttactttccg gctgtgccgg
    13081 aaccagtacg gaatttgagt gtaacgccac cacatccgat acctgtatga cgatggagca
    13141 ggccaatgag aaggccaaaa aactggagca gtcctcagaa gcaaagccgg ttgcggcatc
    13201 actgccgcgc ctggctgaag ggaacttccg gacaatgccg gtgcagaccg tcaccgcgac
    13261 cactccgtcc ggcagcagac ctgcggtgac agcacatccg gagcagaaac tgctggcacc
    13321 tcgcccgctg tttaccgctg cccgggaagt gaaaacggtt gttccggtca gttcagttac
    13381 gccggtaaca cctcctcgtc cgctaagaac gggtgagcag acggcagcat tatggatagc
    13441 gccttatatt gataaccagg atgtttatca tcagccatcc agcgtgtttt ttgttattaa
    13501 accgtctgcg tggggaaaac cacgtattaa ttaactggcc ctgaagttgt atatcagccg
    13561 gcaatccggc gggctttctg tattcaaaat ctgcctgtaa aacgagtgcc ctgtgcgtga
    13621 aaagggatgg gcggtagcgt gtcagatgac catgacagga ataaaccgga tacgccagaa
    13681 aataaatgtt catggtattc ctgtttatct ttgtgaagca tgcggaaatc ctattccgga
    13741 tgcccggcgg aaaatatttc ccggtgtgac gttgtgcgtt gaatgtcagg cgtatcagga
    13801 aaggcagagg aaacactatg cataaatcag aaactgaaaa aatagattct atcctggatg
    13861 aacatcaaca gatatatcgc agacataacc gaatagataa catcctggcg ttttttgcgt
    13921 gtattgcatg gattccctgt attattgctg cgttcggcgt tgatggtata tatctgaaaa
    13981 tcctgtttgc ggtccttgcc tgctgtggtg cggtatgctt ttttattttc ttcttcaccc
    14041 tcctgccgga atcactcatg gtcctcagcc gtcagagcct gcttcagctt atgcgtctga
    14101 ctgaagacgt accggatgcg agacaggaac tgcttaaccg tctgctgtcc ggtaaaaaac
    14161 tgactggtcg ggatgaaaag gatatccggc gtctctggca ggagaaagtg gatgcaatgc
    14221 aggagtctgc cactcgtcag cgtgaacagg ataccatccg gaagtttact gaagagaaaa
    14281 aatccgaatg agaatctgca gtgttttatg acctcataag ttaactgaag ggatattcag
    14341 tgattaatat ataagcggag gtaataaaga atgcatacct ttatttttct tgtgctgata
    14401 aaaaatatta acggtggcgg aagctggaat gttaccccca tgccaaatac ggacgtatgc
    14461 gtgcaggttc ttcggacaat cgaagcacac ggaggcaggg gctttggaaa tacaggggag
    14521 tgtattgaag ttaaaacaaa tgaacctttg aacaacgtga tggatacact gaatgggggg
    14581 caatgagtgc ttactgcttc aaaggaaaat gaaatgaagt cgatatattt aaaatctgtt
    14641 ctggctttta tatttgttgg cgttatggct atgttgatat gtggtctttt ttacaatgat
    14701 taccttgagc aacaacctgc aacaccagaa caactgacag agattataca ggataccccc
    14761 tgtgcagcag aagcatttaa ggaagctatt aagtctgaca catctgatta tcagcctgaa
    14821 cctttaagcc ttggtaaggc aaaaaagctt gcatcagcgt gtagagaaag aaacgaaatg
    14881 gcagaggtta aacgggtaag agaaaatgag cgaaataaga tacgtgaaaa acaactccag
    14941 gcattaaatg atgctcattc agcaaaggaa cattaatcct ggtttcctgt taaaagcctc
    15001 catctcttat ttgtgctgtg gttttaagaa tggcactgcc tgatatatcc acggtcacag
    15061 actgatattt tcctttttta aacgcactaa ggaatactca tatgagtaat aacccacttg
    15121 aggccgtcac tcaggcggtt aactccctcg tcacagcact gaaactgcct gacgaatcag
    15181 caaaggccaa tgaagttctg ggcgaaatga gcttcccgca gttcagccgt ctgctgccgt
    15241 accgtgatta caaccaggaa tccggtctgt tcatgaatga caccacgatg ggctttatgc
    15301 tggaagccat tcccatcaat ggggcgaatg agtctattgt ggaggcgctc gaccacatgc
    15361 tgcgcaccaa actgccgcgc ggagtgccat tctgcatcca tctgatgtcc agccagcttg
    15421 ttggtgacag aattgaatac gggctgcgtg agttctcctg gtccggtgaa caggctgaac
    15481 ggtttaacgc cattacccgt gcctattata tgaacgcggc agcgacacag tttccgctgc
    15541 cggaggggat gaatctgccc ctgaccctgc gccattaccg ggtattcttc tcgtactgtt
    15601 ctccctcgaa gaaaaaaagc cgggctgaca ttctggaaat ggaaaacctg gtgaaaatca
    15661 tccgggcgtc gttacagggg gccagtatca ccacacaggc ggtggatgca caggccttta
    15721 tcgatattgt cggggagatg attaaccata acccggactc cctgtatccg aaaagacgtc
    15781 agctggaccc gtattctgat ctgaattatc agtgtgtgga ggacagtttt gacctgaaag
    15841 tccgggcgga ttacctgacg ctgggcctgc gtgagaacgg caggaacagc acggcccgca
    15901 tcctgaattt ccatctggcc cgtaacccgg aaatcgcctt cctgtggaac atggccgaca
    15961 actacagcaa cctgctgaac ccggaactgt ccatctcctg tccgttcatc ctgacgctga
    16021 ctctggtggt ggaagaccag gtgaaaaccc acagcgaagc caacctgaag tacatggacc
    16081 tggagaaaaa gtcgaagacc tcctatgcca aatggtttcc gtccgtggag aaagaggcga
    16141 aggagtgggg ggaactgcgt cagcggctgg gctccggtca gtcctccgtg gtgtcctact
    16201 tcctcaacat cacagcattc tgcaaggaca ataatgaaac ggcgctggaa gtggagcagg
    16261 acatcctgaa cagctttcgt aaaaacggtt ttgagctgat ttcaccgcgc tttaaccaca
    16321 tgcgcaattt cctgacctgc ctgcccttta tggccgggaa agggctgttt aaacagctga
    16381 aagaggccgg agtggttcag cgcgcagaga gctttaatgt tgccaacctg atgccgttag
    16441 tggcggataa ccccctgaca ccggcaggtc tgctggcacc cacctaccgt aaccagctgg
    16501 cgtttatcga tattttcttc cgggggatga ataacaccaa ctacaacatg gcggtctgtg
    16561 gcacctccgg ggccggtaaa accggactga tacagcccct tatccgcagc gtgctggact
    16621 ccgggggctt tgccgtggtg ttcgacatgg gggatggcta caagtccctg tgtgagaaca
    16681 tgggcggggt gtatctggac ggtgaaaccc tgcgttttaa cccgtttgcg aacatcaccg
    16741 atattgacca gtcagcggag cgtgtccgtg accagttgtc ggtgatggcc agccccaacg
    16801 gtaacctgga tgaagtgcat gaaggtttgc tgctgcaggc ggtcagggcc tcctggctgg
    16861 ctaaagagaa cagggcgcgc attgatgatg tggtggattt cctgaaaaac gcccgtgata
    16921 acgaccagta tgtcgagtca ccgaccatcc gcagccgtct ggacgaaatg attgttctgc
    16981 tggatcaata taccgccggc ggcacttacg gccggtattt taactctgat gagccgtccc
    17041 tgcgggatga tgcgaaaatg gtggtgctgg agctgggcgg actggaagac cgtccgtcac
    17101 tgctggttgc ggtgatgttc agcctcatta tttacatcga gaacaggatg taccgcacgc
    17161 cgcgtaacct caaaaaactg aacgtcattg atgagggatg gcgtctgctg gacttcaaaa
    17221 atcacaaggt cggtgagttt attgagaaag gttaccgtac cgcccgccgt cataccggtg
    17281 cctatatcac catcacacag aacatcgtcg actttgactc tgacaaggcc tccagtgctg
    17341 cccgcgcggc atggggtaac tcctcctaca aaattatcct caaacagagt gcgaaggagt
    17401 tcgcgaaata caaccagctg tatccggacc agttcctgcc actgcagcgt gacatgattg
    17461 gtaagttcgg tgcggccaaa gatcagtggt tcagttcctt cctgctgcag gtggaaaacc
    17521 attcctcctg gcaccgcctg tttgtggacc cgttaagccg cgccatgtac agctctgacg
    17581 gcccggattt tgagtttgtg cagcagaaac gtaaagaggg gctgagtatt catgaggcgg
    17641 tgtggcagct ggcgtggaag aagtcagggc cggagatggc ttcgctggaa gcctggctgg
    17701 aagaacatga gaaatacagg agtgttgcat gaccacaacg caaaaaacga ctgacgttac
    17761 ggccccccaa cgcagccact ggtggtggac ggtgccggga tgtctggcaa tggtgttact
    17821 gaacgcagct gtcagttacg gcattgtcag actgaacgca ccggtgactg ccgccttcaa
    17881 catgaaacag accgtggatg cgttttttga cagcgccagt cagaaacaac tgtcagaagc
    17941 acagtcaaaa gccctttcag cgcgttttaa cacggcactg gaagccagcc tgcaggcgtg
    18001 gcagcagaaa caccacgcgg tcattctggt gtcgcctgct gtggtacagg gggcaccgga
    18061 tatcacccgg gaaatccagc aggatattgc ccggcgaatg agggcggagc catgagatgc
    18121 cgggggctga ttgccctgct gatatggggg cagagtgtgg ccgccgccga tcttggtacc
    18181 tggggcgatc tgtggccggt aaaggagccg gacatgctga cggtgatcat gcagcgcctg
    18241 acggcactgg agctgtccgg tgagatgggc cggaaaatgg atgcctttaa ggagcgggtg
    18301 atccgcaaca gtctgcggcc ccctgccgtg cccggtatcg gacgcacgga gaaatacggt
    18361 agccggttgt ttgacccgtc cgtcagactg gctgcggata tccgggataa cgaagggcgg
    18421 gtgtttgccc gtcagggtga agtgatgaat ccgctgcagt atgttccttt taaccagacg
    18481 ctgtatttca tcaatggcga tgatccggcg caggtggcct ggatgaaacg ccagacgccg
    18541 cccacactgg agagcaaaat catcctcgtg cagggcagta tcccggagat gcagaagtct
    18601 ctggacagcc gtgtttactt tgaccagaac ggtgtgctct gccagcgcct cggcattgat
    18661 caggtcccgg cacgggtgag cgccgttccc ggcgatcgct tcctgaaggt ggaatttatt
    18721 ccggcagagg agggcagaaa atgaagcgaa gcctgtggct gctgatgtta ttccttctcg
    18781 ccggtcatgt ccctgcagcg tctgcggatt ctgcctgtga ggggcgtttt gtaaacccga
    18841 tcacggatat ctgctggagc tgtattttcc cgctctcgct gggcagtatc aaagtcagtc
    18901 agggtaaggt ccccgacacg gcgaacccgt cgatgcccat tcagatttgt ccggcaccgc
    18961 cgccgctgtt caggcgtatc ggactggcca ttggttactg ggagccgatg gcgctgacgg
    19021 acgtcacccg gtcaccggga tgtatggtga acctgggctt cagcctgccg gcttttggta
    19081 aaacggcaca gggaacggcg aaaaaagatg agaagcaggt gaacggggcg ttctatcacg
    19141 ttcactggta caaatacccg ctgacgtact ggcttaacat catcacatcg ctgggctgtc
    19201 tggaaggtgg tgacctggat atcgcttatc tttctgaaat cgaccccacc tggacggaca
    19261 gcagcctgac caccattctc aatccggaag ccgtcatctt tgccaatccg atagcacagg
    19321 gggcctgtgc tgcagatgcc attgccagcg cctttaacat gcccatcgat gctctgttct
    19381 ggtgtgccgg ttcgcaggga agtatgtacc cgttcaatgg ctgggtgagt aatgagtcca
    19441 gtccgttgca gtcctccctg ctggtcagtg aacgcatggc gttcaagctg caccgtcagg
    19501 gcatgattat ggaaaccatc gggaaaaata acgccgtctg taatgaatat ccgtccccaa
    19561 tcctgcccaa agaacgctgg cgttaccaga tggtgaatat gtatccggac agcgggcagt
    19621 gccacccgtt cgggcgcagc gtgacccgct gggaaaccgg gaaaaatccg cccaatacaa
    19681 agaaaaactt cggctacctg atgtggcgta aacgtaactg tgtcttcctg tgaggtgaat
    19741 gatgaagctg agtatgaaat ctctggcagc actgctgatg atgctgaacg gggcggttat
    19801 ggcgtcagaa aacgtgaaca ctcctgagaa ccgccagttc ctgaagcagc aggaaaattt
    19861 aagcagacaa ctgcgtgaaa aacctgacca tcagctgaaa gcctgggcgg agaagcaggt
    19921 gctggaaaac ccccttcagc gttcagataa ccatttcctg gatgagctgg ttcgtaaaca
    19981 gcaggcttcg caggacggga aaccccggca gggtgctctg tattttgtgt cgttttccat
    20041 tcccgaagag gggctgaaac gaatgctggg cgaaacccgg cactacggta tacccgccac
    20101 actgcggggc atggtgaaca atgacctgaa gaccaccgct gaagccgtgc tgtccctggt
    20161 gaaagacggc gcgactgatg gtgttcagat cgacccgacg ctgttttcac agtacggcat
    20221 tcgcagtgta ccggcactgg tggtgttctg cagtcaggga tacgacatca tccggggaaa
    20281 cctgcgggtc ggccaggcgc tggagaaagt ggccgccacg ggtgactgcc ggcaggtggc
    20341 tcatgattta ctggcaggga aaggagattc cgggaaatga aacgtatttt acctctgata
    20401 ctggctctgg ttgccggcat ggcacaggct gacagcaaca gtgattaccg ggccggctct
    20461 gattttgctc accagatcaa aggacaggga agcagcagta ttcagggctt taagccacag
    20521 gagagtatcc ccggctataa cgcgaatccg gacgagacaa aatactacgg tggcgtgact
    20581 gccggcgggg atggcggcct gaaaaatgac ggcaccacgg aatgggcgac cggtgaaacc
    20641 ggaaaaacca tcacagagtc attcatgaac aagccgaaag acattctttc accggatgca
    20701 ccgtttatcc aggccggcag ggatgtggtg aaccgggctg acagcattgt gggaaacacc
    20761 gggcagcagt gcagtgcgca ggagattagc cggagtgaat acacgaatta cacctgtgag
    20821 cgggatttac aggtggagca gtactgcaca cgaactgccc gtatggagct gcaggggagt
    20881 accacatggg aaacccggac gctggagtat gaaatgagtc agctacctgc acgtgaagtg
    20941 aatggtcagt atgttgtctc cattacctct cccgttaccg gtgaaattgt cgatgcgcat
    21001 tacagctgga gtcgtactta cctgcagaag agtgtaccta tgacaattac agtactggga
    21061 accccactga gctggaatgc caaatactca gcagatgcct catttacacc agtacagaaa
    21121 acactgactg ccggtgttgc ttttacgtca tctcaccccg tccgggtcgg gaatacaaaa
    21181 ttcaaacgtc atacggcaat gaagctgcgt ctggttgtca gggtaaaaaa agcctcgtac
    21241 accccgtatg ttgtctggtc tgaaagctgc ccgttcagca aggagctggg gaaactgaca
    21301 aaaacagaat gtacggaggc tggcgggaac aggacgctgg tgaaagacgg tcagtcatac
    21361 agcatgtacc agaactgctg ggcataccgg gacacgtatg tcacacagtc agcagacaag
    21421 ggaacctgtc agacctacac cgacaatcct gcctgtaccc tggtgtcaca ccagtgcgcc
    21481 ttttactccg aagaaggtgc ctgtctgcat gaatatgcca cgtactcctg tgagtcaaaa
    21541 acatccggga aagtgatggt ctgtggggga gacgtcttct gtctcgatgg cgaatgcgat
    21601 aaggctcaga gcgggaagag taatgatttt gccgaagctg tttctcagct ggcggcactg
    21661 gccgcagcag gtaaggatgt tgcggcgctg aacggggtgg atgtccgtgc tttcaccggt
    21721 caggcgaaat tttgtaagaa ggctgcagcc ggatacagca actgctgtaa ggacagcggc
    21781 tggggacagg atatcgggct ggccaaatgc agcagtgatg aaaaagccct ggctaaagca
    21841 aaatcaaaca aactgaccgt cagtgtcgga gagttctgtt cgaagaaagt gctgggtgtc
    21901 tgcctggaga aaaaacgcag ttactgtcag tttgattcga agctggcgca gatagtccag
    21961 cagcaggggc gcaacggaca gctgcgtatc agttttggca gtgcgaagca tcctgactgt
    22021 cgggggatta cggttgatga gctgcagaaa attcagttcg acagactgga tttcactaac
    22081 ttctacgaag acctgatgaa taaccagaaa atcccggaca gtggtgttct gacgcagaaa
    22141 gtgaaagagc agattgctga ccagctgaaa caggcaggac agtaaggtat ggaaaataac
    22201 cggggaaaat gatgaaagtc atattcacgt cgaaccgttt tattgatttt cttatccggc
    22261 ttttgattac agcgattgtc atttccccgg taattatctg gtcgtgggat acggtaaaag
    22321 aaaccactgc aggtgagatg cttgctgcct gttttgttat tttttactcc ggtgtgctcc
    22381 tgtttattct gtatttttgt ttttctgccc tgactgacct gcagaaatct gatgaaagaa
    22441 aaagtgatga aaggaatgaa gatgaataaa gcattactgc cactgttact ctgctgcttt
    22501 atttttccgg cgtcagggaa agatgcaggc tggcagtggt ataacgagaa aataaatcca
    22561 aaggaaaaag aaaataaacc tgtacctgca gtcccacgtc aggagccgga tattatgcag
    22621 aaactggccg cactgcagac ggcaacaaag cgggcgctgt acgaagctat tctgtatccc
    22681 ggcgtggata attttgtgaa atatttccgg ctgcagaatt actggactca gcaggccggg
    22741 cttttcacca tgagcgccaa aaaagccatg ctggcgcatc ccgaactgga ctataacctg
    22801 cagtacagcc attacaacgg cacggtacgg aaccagctgg cagcagacca ggcgcagcag
    22861 cgacaggcta ttgcgaaact ggctgaacac tacggcatca tgttttttta ccgggggcag
    22921 gaccccatcg acgggcagct ggcgcaggtc attaatggct tcagggatac ctatggtctg
    22981 agtgttattc ccgtttccgt ggatggtgtg attaatccgc tgttgccgga ttcccggact
    23041 gaccaggggc aggcgcagcg cctcggcgtg aaatatttcc cggccatgat gctggttgac
    23101 ccgaaacagg gcagtgttcg tccgttatca tacggtttta tttcgcagga cgacctggca
    23161 aaacaattcc tgaacgtttc tgaagatttt aaacccaatt tttaatcgcg gatttgattt
    23221 atgagtgaag attgtttgaa aatgtttaca ggtgttgttc tgttaatatt tgtcattatt
    23281 gccggttatt tcttttctga gcgtaatgac aggaaaatgt ttctcctgag ctcactggtt
    23341 ttccttgtta ttaatatcgc gtgtttatat gtgctgactg ccagattctg gtttctgtgt
    23401 ggtgcaatta tgaatcaggg cgcagcaatg gttgttccaa tggttattgg ctcattaccg
    23461 aacgttacga gcttcgacgg gttcagaaga atatttatct gtattatgtt gtcatcagta
    23521 tggtccggag tgatgtggtt ttttataagg gggcttatga caggctaagt catataaaac
    23581 ggtaataaaa accatacaaa ataatgaggt tgttctgatt aatatcacac aaacgcgaat
    23641 attacagaca taaatactct catgtaaata ataaccggag tttaacatga taagtaaacg
    23701 cagattctct ttgccccggc ttgatattac gggaatgtgg gtattttccc tgggtgtctg
    23761 gtttcatatc gtcgcccgtc ttgtttacag caaaccctgg atggcttttt ttctggcgga
    23821 gcttattgcg gccattctcg tgctgttcgg cgcataccag gtgctggatg catggattgc
    23881 ccgtgtcagc cgggaagagc gtgaggcact ggaagccagg caacaggcca tgatggaagg
    23941 gcagcaggag ggcagacatg tctctcatta aatcactgct gttcaccctg ttgctggcag
    24001 ccgctgccgt gcaggcctcc acccgggatg aaattgaacg actctggaat ccgcagggca
    24061 tggccgcgca gcctgcacaa ccggcagcag gcacgtcagc caggacagaa aagccagccc
    24121 cccgctggtt ccgcctcagc aacggcaggc aggttaacct ggccgactgg aaggtggtgc
    24181 tgtttatgca ggggcattgc ccttactgtc accagtttga cccggtactg aaacagctgg
    24241 cacagcagta cggcttttcc gtttttccct acaccctgga tggtcagggg gataccgcct
    24301 ttccggaggc attgccggtg ccaccggacg tgatgcagac cttcttcccg aatatcccgg
    24361 tggccacacc gaccaccttc ctggtcaacg tcaacacgct tgaggcatta ccgcttttac
    24421 agggggcaac ggatgctgcc ggttttatgg cgcggatgga taccgttctg cagatgtacg
    24481 gagggaaaaa aggtgcgaaa taagcaggtg gtgttactca ttgccggaat atccggcatc
    24541 gcgacgggaa taatcgtcag cctgaatatc ccctttatcc atcaggggct tttttatccc
    24601 gccagccctg tagaaattgt cgtttcgctg tgccttacct tttctgtttc tgttgtgttt
    24661 tttgtggggg caattgtggg atggatttcg gtgtctgaaa tatattacag ccgcaggact
    24721 ggacggaaat atcgggatat tcctgctgaa atgtcgtatg cgccgggaga acacattaag
    24781 ggagggcaag aatgatgcca cgcattaaac ctcttctggt tctttgtgct gctttactga
    24841 cggtcacacc cgcagcgtca gcggatgtga acagcgacat gaatcagttc tttaacaagc
    24901 tgggcttcgc ctccaatacc acacagccgg gcgtctggca ggggcaggcc gccggttatg
    24961 cttacggtgg ctccctgtat gcccgtactc aggtcaaaaa tgttcagctg atttccatga
    25021 cgctgccgga tatcaatgcc ggatgtggcg gcatcgacgc ctatctcggc tcgttcagtt
    25081 ttattaatgg cgagcagctg cagcgttttg ttaagcagat tatgagcaat gctgccggtt
    25141 acttttttga ccttgccctg cagacaacgg tgccggaaat caaaaccgca aaagacttcc
    25201 tgcagaaaat ggcaagcgac attaacagta tgaacctcag ttcctgccag gcggcacagg
    25261 ggattatcgg cgggcttttc ccccggacgc aggtgtccca gcagaaagtc tgtcaggaca
    25321 ttgccggtga gagcaatatt tttgctgact gggcggcttc ccggcaggga tgtaccgttg
    25381 gcgggaaatc tgacagcgtc agggataaag ccagcgacaa ggataaggag cgggtgacca
    25441 aaaacattaa catcatgtgg aatgcgcttt ccaaaaacag aatgtttgac ggcaacaaag
    25501 agctgaaaga gtttgtgatg acgctgaccg gctcactggt gtttggtccc aacggcgaaa
    25561 tcacaccgct gtcagccaga accactgacc gctcaattat ccgggccatg atggaaggcg
    25621 gcacagcaaa aatttatcac tgcaacgatt ctgataaatg cctgaaagtg gtggcagaca
    25681 caccggtgac catcagccgg gataatgcac tgaagtctca gattactaaa cttctggcca
    25741 gcattcagaa caaggcggtc agtgacacac ctctggatga caaggaaaaa ggctttattt
    25801 ccagtaccac catccccgtc ttcaaatacc tggttgaccc gcagatgctc ggtgtttcca
    25861 acagtatgat ttaccagctg acggactata tcggttacga catcctgctg cagtacattc
    25921 aggagctgat acagcaggcc cgggcgatgg tggccacagg aaattatgac gaagcagtta
    25981 tcgaacatat taacgacaac atgaatgatg ccacccggca gattgcggcg tttcagtcac
    26041 aggtgcaggt acagcaggat gcgctgctgg ttgtcgatcg tcagatgagc tacatgcgtc
    26101 agcagctttc cgcccgcatg ctcagtcgtt accagaacaa ctatcacttc ggagggagca
    26161 cgctgtgaat gaagtttatg tgattgccgg tggtgagtgg ttgcggaata acctgaacgc
    26221 cattgccgcc tttatgggaa ccaggacgtg ggattccatt gaaaaaattg cgctcacatt
    26281 gtctgttctc gcggtggccg tgatgtgggt acagcggcac aacgtgatgg atttgctggg
    26341 ctgggtggcc gtgtttgtgc ttatcagcct gctggttaat gtccgcacat cggtgcagat
    26401 tattgataac agtgacctgg tcaaagttca ccgggtggat aatgtaccgg tcggactggc
    26461 gatgccactt tcactgacga cccgtatcgg gcatgcaatg gtggccagtt acgagatgat
    26521 cttcacgcaa ccggacagtg tcacctacag caaaacgggg atgctgtttg gcgcagaact
    26581 ggtatcaaaa agcaccgact ttttgtcccg caatccggaa atcgccaatc ttttccagga
    26641 ttatgtgcag aactgcgtga tgggggatat ttacctgaac cacaaataca cgctggagga
    26701 gctgatggcc tccgctgatc cctacacgct gattttttcc cgccccagtc cgctacgggg
    26761 cgtttatgac agcaataata atttcgtgac ctgtaaggac gcgtcggttt cgctgaaaga
    26821 caagctgaat ctggatacac agagtggcgg aaaaacctgg cattattatg cccagcagct
    26881 atttggtgga cgccctgatc cgaacctgtt gttcagtacg ctgattggcg acagttacag
    26941 ttacttctat ggctcaagta aatcagccag ccagatcatc cgccagaatg tcaccatcaa
    27001 tgcgctgaag gaggggatca ccagttatgc cgcgcgtaat ggtgattcag ccagcctggt
    27061 gaatctggcc accacgtcat cgatggagaa gcaacgtctg gcacatgtct ccatcggtca
    27121 tgtggcgatg cggacactgc ccatgacgca gaccatcctg acggggatcg ccatcggtat
    27181 tttcccgctt ctggtactgg cggctgtctt caacaaactg acgctgtccg tcctgaaagg
    27241 ctatgtgttt gccctgatgt ggttgcagtc atggccaatg ttgtacgcca ttctgaacag
    27301 cgccatgaca ttttatgcga agcagaatgg tgcaccggtg gtgctctcgg aaatttcaca
    27361 gatacagctg aaatactccg atctggcttc cactgccggg tatctttcca tgatgatccc
    27421 gccattatcc tggatgatgg tcaggggact cggggccgga ttttccagtg tgtacagcca
    27481 tttcgcttcc tctgctatca gcccgactgc cagtgcggca ggtagtgtgg ttgacggtaa
    27541 ttactcctac ggcaacatgc agacggaaaa cgtgaacgga ttcagctgga gcaccaacag
    27601 caccacgtcg tttggtcaga tgatgtacca gaccggcagt ggcgcaaccg ccacacagac
    27661 ccgtgacggt aatatggtga tggatgcgag cggggcgatg tcccgtttac cggtcggtat
    27721 caatgcaacg cgtcagattg cggcggcaca acaggaaatg gcccgggagg cgtcgaacag
    27781 agcagaaagc gccctgcatg ggttcagcag cagtattgcc agtgcctgga actcgctcag
    27841 tcagtttggt tctaaccggg ggagcagtga ttctgtcacc ggtggtgctg acagcacgat
    27901 gagcgcacag gactccatga tggccagccg tatgcgcagc gcagtggaaa gctatgcgaa
    27961 ggcgcataat atcagtaatg agcaggcgac aagagaactg gcatcaagaa gtacgagagg
    28021 ttctgcgggg atgtatggcg atgctcatgc tgaatggggg gttaaaccta aaattcttgg
    28081 tgttggtggt ggcgtaggtg ttagaggtgg tggtcgggca agtattgact ggagtgatga
    28141 ggatgcgcat caggccagca gtggttcacg ggccagccat gatgctcgcc atgatatcga
    28201 tgccagggct acacaagact ttaaagaggc cagcgattac tttaccagtc gcaaggtcag
    28261 tgaatccggc agccatacgg acaataatgc cgattcccgt gtggaccagc tgtctgcagc
    28321 cctgaactca gcaaaacaga gttacgacca gtacacgacg aatatgaccc gcagccatga
    28381 atatgctgaa atggcatccc gtactgaaag catgagtggg cagatgagtg aagacctgtc
    28441 gcaacagttt gcacagtatg tgatgaaaca cgcgccgcag gatgccgaag ctattctgac
    28501 aaacaccagt tcaccggaga ttgcagaacg ccgtcgggct atggcgtggt cttttgttca
    28561 ggaacaggtg cagcctggtg ttgataatac ctggcgtgaa tcccgtgggg acatcggtaa
    28621 aggaatggag agcgtacctt cggggggggg cagccaggat attattgctg atcatcaggg
    28681 gcatcaggcc attattgagc aaagaacgca ggacagtaat atccgtaatg atgtaaaaca
    28741 tcaggttgat aatatggtca cagaataccg aggcaatatc ggagatactc agagcagcat
    28801 acgtggcgaa gaaaatattg taggaagaca gtattctgaa ctacaaaacc accataaaac
    28861 agaggcatta tctcagaata ataaatataa tgaagaaaaa tcgggtcagg aaagaatgcc
    28921 cggggctgac agtccacagg aactgatgaa aagagcaaag gagtatcagg ataagcacaa
    28981 gcaataatat tgtatgggga gatgataatg aaaaatttag ctcatattgc tctggtgacg
    29041 gtgatacagt tcattgcctg ttatctggct gattggggga gtgccgaaac agtttttatc
    29101 ctgtgcttta ttgtactatg gcagggattg tttatatggt tattttctca aatacgaaaa
    29161 aaacggcatg tgtcggatga gtttaaattc agtaaaggcg tttggtatat actgatgcca
    29221 gtatcttcct tactctctcc gttattaagc ctgatggttt ttatcattgg tacactatac
    29281 gaattaaggc gaattagtgg ttgtgtaagt gtaagagaat ggatgcaatc tcaggtaaat
    29341 gaacaaacta atgaagattt gcatctggat tttgatgatg tgaattttga ttatgtggat
    29401 ttttatcgta ctaatccggc aacaggatta gctatgcatg gaggatttga ttcagcagga
    29461 aatacttttg ggacttgttg gcaggactat gatgacaggc gataagaaaa ctgtggtttg
    29521 taataaaggt aatttgatga aaacaaaaaa attgatgatg gttgcactgg tcagttccac
    29581 tctggccctt tcagggtgtg gtgcgatgag cacagcaatc aagaagcgta accttgaggt
    29641 gaagactcag atgagtgaga ccatctggct tgaacccgcc agcgaacgca cggtatttct
    29701 gcagatcaaa aacacgtctg ataaagacat gagtgggctg cagggcaaaa ttgctgatgc
    29761 tgtgaaagca aaaggatatc aggtggtgac ttctccggat aaagcctact actggattca
    29821 ggcgaatgtg ctgaaggccg ataagatgga tctgcgggaa tctcagggat ggctgaaccg
    29881 tggttatgaa ggcgcagcag ttggtgcagc gttaggtgcc ggtatcaccg gatataattc
    29941 cagttctgcc ggtgccacac tcggtgtggg ccttgctgcc ggtctggtgg gtatggctgc
    30001 agatgcgatg gtggaagatg tgaactatac catgatcacg gatgtgcaga ttgcagagcg
    30061 tactaaggca acggtgacaa cggacaatgt tgccgccctg cgtcagggca catcaggtgc
    30121 gaaaattcag accagtactg aaacaggtaa ccagcataaa taccagaccc gtgtggtctc
    30181 aaatgcgaac aaggttaacc tgaaatttga agaggcgaag cctgttctcg aagaccagct
    30241 ggccaaatca atcgcaaata ttctctgatt atcttccgga ggctggtctg accggcctcc
    30301 tgactgacgc ctcgctttgc tcgttgtcca accccggaaa gaaataaaat aaattttatt
    30361 tctccccggc gtttccgaaa taaatatgaa aacactgagc ggttgtgcaa attctttgtg
    30421 cgaccgtgaa gtgttgccgg cgaagccgga atttaacggt tattaatctt ttcatcatta
    30481 ataatcagtc cgactgactt tttttcttcg gaatatcatc atgagtttta acgcaaagga
    30541 tatgacccag ggcggtcaga ttgcgtccat gcgtatccgc atgttcagcc agatcgccaa
    30601 tatcatgctt tactgcctgt ttattttttt ctggatactc gttggtctgg ttttatgggt
    30661 aaaaataagc tggcagacgt ttgtgaacgg ctgtatttac tggtggtgta ccacgctgga
    30721 aggcatgcgg gatttaatca agtcccagcc ggtctatgag atccagtatt acggcaaaac
    30781 cttccggatg aatgctgctc aggtactgca tgataaatat atgatctggt gcggagagca
    30841 gctatggtcc gcattcgttc tggccacagt tgtggcactg gttatttgcc tgattacctt
    30901 ctttgttgtc tcctggattc tggggcgtca gggtaaacaa cagagcgaaa atgaagtcac
    30961 aggtggtcgt cagctgacag acaatccgaa agacgttgcc cggatgctga aaaaagacgc
    31021 caaggactcc gatatccgga ttggcgacct gccgattatc cgggattctg aaatccagaa
    31081 cttctgtctg cacggcacgg tcggggccgg taagtcggaa gttatccgtc gtctggccaa
    31141 ctacgcccgt cagcgcggtg atatggtggt gatttatgac cgttcagggg aatttgttaa
    31201 aagttactat gatccctcca tcgataagat cctgaatccg ctggatgccc gctgtgccgc
    31261 ctgggattta tggaaggagt gtctgacaca gccggatttt gataatacgg caaataccct
    31321 gatcccgatg ggcacaaaag aggacccgtt ctggcagggt tcaggacgta ccatttttgc
    31381 ggaagcggcg tacctgatgc gtaatgaccc caaccgcagc tacagcaaac tggtggacac
    31441 actgctttcc atcaaaatcg aaaaactgcg taccttcctg cgtaattcac cggcggccaa
    31501 cctggtggaa gagaaaattg agaaaacggc gatttccatc cgtgctgtgc tgaccaacta
    31561 cgtgaaagcc atccgttacc tgcaggggat tgagcataac ggtgagtctt ttaccatccg
    31621 tgactggatg cggggtgtcc gggaagatca gaaaaacggc tggctgttta tctcgtcgaa
    31681 tgccgacacc catgcctccc tgaagccggt gatctccatg tggctgtcca ttgccattcg
    31741 tggtctgctg gcaatgggag aaaaccgtaa ccgtcgtgtg tggtttttct gtgatgagct
    31801 acccacgtta cacaaactgc cggacctggt ggagatcctg ccggaagccc gtaagttcgg
    31861 tggctgttat gtgtttggta tccagtccta tgcccagctg gaagatatct acggtgagaa
    31921 agcggctgcc acgctgtttg acgtcatgaa cacccgtgcc tttttccgtt ctcccagcca
    31981 taagattgca gagttcgctg ccggtgagat tggtgagaaa gagcacctga aagccagcga
    32041 gcagtactcc tacggtgctg atccggtacg tgacggggta tcgaccggta aggatatgga
    32101 gcgccagacg ctggtcagtt attccgacat tcagtctctg ccggatttga cctgttatgt
    32161 caccctgccc ggaccgtatc cggcagtaaa actctcactg aaatatcagg cacgtccgaa
    32221 ggttgcccct gagtttattc cgcgtgacat caacccggaa atggagaacc gcctgagcgc
    32281 cgtacttgcc gcaagggaag cagaaggtcg tcagatggcc agcctcttcg aaccggatgt
    32341 gccggaagtt gtttccggag aagacgtgac tcaggctgaa cagccgcaac agccgcaaca
    32401 gccgcaacag ccgcaacagc cgcaacagcc ggtgtctcct gccatcaacg ataagaagtc
    32461 agattcaggt gtgaatgttc cggcaggggg gatcgagcag gagctgaaaa tgaaaccgga
    32521 agaagagatg gaacagcaac tgccacccgg gatcagtgaa tccggtgaag tggtggatat
    32581 ggccgcttat gaggcatggc aacaggaaaa tcatccggac atccagcagc agatgcagcg
    32641 tcgtgaagag gtgaacatta atgtgcaccg ggagcgtggg gaggatgttg agccgggaga
    32701 tgatttctga tgatgagtat cgcgcaggtc agatcggccg gaagtgccgg taactattat
    32761 accgacaagg ataattacta tgtactgggc agcatgggag aacgctgggc cggcaggggg
    32821 gctgaacagc tgggactgca gggcagtgtc gataaggatg tttttacccg tcttctggag
    32881 ggaaggctgc cggacggagc ggatctaagc cgcatgcagg atggcagtaa taagcatcgt
    32941 cccggctacg acctgacctt ctccgccccc aaaagtgtct ccatgatggc catgttaggt
    33001 ggcgataagc gcctgattga tgcacataac caggccgtgg actttgctgt tcgtcaggtg
    33061 gaggcgctgg cctccacacg ggtgatgacg gacggacagt cagaaacggt actgaccggt
    33121 aatctggtga tggcactgtt taaccacgac accagtcgcg atcaggaacc acagttacac
    33181 acgcatgcgg tggtggctaa tgtcacgcag cataacggtg agtggaagac actgagcagt
    33241 gacaaagtgg ggaaaacggg gttcattgag aatgtgtacg ctaatcagat tgcctttggc
    33301 aggctctacc gggaaaaact gaaagagcag gttgaggcgc tgggctatga aaccgaagtg
    33361 gtgggtaagc acggtatgtg ggagatgccg ggcgtaccgg tggaggcctt ttccggacgc
    33421 tcacaggcta tccgggaggc cgtcggggaa gatgcctcgc tgaaatcccg ggatgtggcg
    33481 gcactggata cgcgaaaatc caaacagcac gtcgatcctg aagtcagaat ggctgaatgg
    33541 atgcagacgc tgaaggaaac cgggtttgac atccgggcat atcgtgacgc ggcggatcag
    33601 cgtgcggaga cccgcacgca ggcgcccggg gctgtttcac aggaggggcc ggatgtgcag
    33661 caggcggtga cacaggcgat tgccggatta agtgaacgaa aagtgcagtt tacgtacacg
    33721 gacgtgctgg ccagaacggt cggtatactg ccgccggaaa atggtgtgat tgaacgggca
    33781 cgcgccggta tcgacgaggc catcagccgt gagcaactca tcccccttga ccgtgagaag
    33841 gggctgttca cgtccgggat taatgtgctc gatgaactgt cagtccgggc actcagtcgc
    33901 gatatcatga aacagaaccg ggtgaccgta catccggaga aaagtgtccc ccggacggcc
    33961 ggctacagcg atgccgtcag tgttctggca caggaccgtc cgtcgctggc cattgtgtcc
    34021 gggcagggcg gtgcagccgg gcagcgtgag cgggtggctg aactggtcat gatggcccgg
    34081 gagcaggggc gggaagtgca gattatcgct gctgaccgtc gctcgcagat gaacctgaag
    34141 caggatgaac ggctgtccgg tgagctgata accggacgtc gtcagctgct ggaaggcatg
    34201 gctttcacgc cgggcagtac cgttatcgtt gaccagggcg aaaaactctc cctgaaagag
    34261 acgttaaccc tgctggacgg tgccgcacgt cataacgtac aggtcctgat aaccgacagt
    34321 gggcagcgaa ccggtacagg cagtgcgctg atggccatga aggatgccgg ggtgaacaca
    34381 tatcgctggc aggggggaga acagcgaccg gccaccatca tcagtgaacc ggaccgtaat
    34441 gtccgctatg cccggctggc cggagatttt gcggccagcg tgaaagccgg agaagagagc
    34501 gtggcacagg tcagcggagt acgggaacag gccatactga cacaggccat tcgcagtgag
    34561 ctgaaaacac agggcgtgct cggacacccg gaggtaacca tgaccgccct gtcaccggtc
    34621 tggctggaca gccggagccg ttatctgcgg gatatgtacc gtccggggat ggtgatggag
    34681 cagtggaacc cggagacacg cagtcatgac cgctatgtta tcgaccgggt gacggcgcag
    34741 agtcacagcc tgaccctgcg ggatgcgcag ggtgaaacgc aggtggtgcg tatttcctcc
    34801 ctggacagca gctggtcgct gttccggccg gaaaaaatgc cggtggcaga cggcgagcga
    34861 ctgagggtga cagggaaaat tcccggactc cgcgtctccg gcggtgaccg cctgcaggtg
    34921 acatctgtca gtgaagatgc gatgacggtt gttgtgccgg ggcgggctga accggcctcc
    34981 ctgcctgtgg ctgattcacc attcacggca ctgaagctgg agaacggctg ggtggaaacg
    35041 cccgggcatt ccgtcagcga cagtgcgaag gtttttgcct ccgtcacaca gatggcaatg
    35101 gacaacgcca ccctgaacgg tctggcccgc agcggtcgtg atgtccggct gtattcctca
    35161 ctggatgaaa cccgtactgc ggaaaaactt gcccgccatc cctcctttac ggtggtttct
    35221 gagcagataa aggcgcgtgc cggtgagaca ttgctggaaa ccgctatcag tctgcagaaa
    35281 accgggcttc acacgccggc acagcaggct attcatctgg cgcttcctgt ggtggaaagt
    35341 aaaaacctgg ccttcagcat ggtggacctg ctgacagagg cgaagtcgtt tgctgcagaa
    35401 ggaaccagtt ttactgaact gggaggggaa atcaatgcgc agataaaacg cggtgactta
    35461 ctgtatgtgg atgtggcaaa aggctatggc acaggcctgc tggtttcccg tgcgtcgtat
    35521 gaggcagaaa agagcattct tcgccatatt ctcgaaggta aggaggcggt cacgccgctg
    35581 atggagagag tacctggcga actcatggag aaactgacat caggacagcg cgccgccacc
    35641 cgtatgatac tggaaacgtc cgaccgtttc acggtggtac agggttatgc cggtgtgggt
    35701 aagaccacac agttccgggc ggtgatgtca gccgtgaaca tgctgtcggc gagtgagcgt
    35761 ccccgagttg tggggctggg gcccacgcac cgtgcggtcg gtgagatgcg cagcgccggc
    35821 gtggatgctc agacactggc gtcctttctg catgacacgc agctgcagca gcgcagcgga
    35881 gaaacgccgg atttcagcaa cacgctgttc ctgctcgatg agagctctat ggtgggcaat
    35941 accgacatgg cacgggcata cgccctgatt gcggccggtg gcggtcgggc tgtggccagc
    36001 ggtgacacgg accagctgca ggccatcgcg cccggtcagc ctttccgtct gcagcagacg
    36061 cgcagtgctg ccgatgtggc catcatgaag gagattgtgc gtcagacgcc ggaactgcgg
    36121 gaggcggtat acagcctgat taaccgggat gtggaaaggg cactgtccgg gcttgagagt
    36181 gtgaaaccgt ctcaggtgcc acgtctggag ggcgcatggg caccggagca ctccgtgacg
    36241 gagttcagtc acagccagga agcgaaactg gcagaagcgc agcagaaggc gatgctgaaa
    36301 ggcgaggctt tcccggatgt ccccatgaca ctgtatgaag ccattgtccg cgactatacc
    36361 ggcagaacac cggaagcacg ggagcagacg ctgattgtca cgcacctgaa tgaggaccgg
    36421 cgcgtgctga acagcatgat tcatgatgca cgggaaaagg ccggtgagct gggaaaagag
    36481 caggtcatgg tgcctgtcct gaacacagcg aatatacgcg acggggagct gcgtcgtctc
    36541 tccacctggg agacacatcg ggacgcactt gccctggtgg ataatgtgta tcaccggatt
    36601 gccggtatca gcagggatga cgggctgata accctgcagg atgctgaagg taacacgcgg
    36661 ctgatttcgc cccgggaggc ggtggctgaa ggcgtcacac tgtacacccc ggacaccatc
    36721 cgggtgggga ccggtgaccg gatgcgcttc acgaagagtg accgggagcg cggttatgtg
    36781 gccaacagcg tctggacggt gacagcggtt tctggtgaca gtgtcacact ttctgacggt
    36841 cagcagaccc gggtgattcg ccccggtcag gagcgggcag agcaacatat tgacctggcc
    36901 tatgccatca ccgcccacgg tgcgcagggg gcaagtgaaa cctttgccat cgcgcttgaa
    36961 ggcacggaag gtaaccggaa actgatggca ggctttgagt cagcctacgt ggccctgtcg
    37021 cgtatgaagc agcatgtgca ggtgtacacc gataaccgtc agggctggac ggatgccatc
    37081 aacaatgccg tacagaaagg aactgcccac gatgtgtttg agccgaaacc ggaccgggag
    37141 gtcatgaatg cagagcggct gttcagtacg gcgcgggagc tgcgggacgt ggcggcaggc
    37201 cgagccgttc ttcgtcaggc ggggctggcc gggggagaca gtcctgcacg gtttattgct
    37261 ccggggcgca aatacccaca gccgtatgtg gcactgccgg cgtttgaccg taacggcaag
    37321 tctgccggca tctggctgaa cccactgacc acggatgacg gaaacgggct gcggggattc
    37381 agcggtgaag gccgggtgaa aggcagcggg gatgcgcagt tcgtggccct gcagggcagc
    37441 cgtaacggcg agagcctgct ggcggataac atgcaggatg gcgtccggat tgcccgtgat
    37501 aaccctgaca gcggtgtggt ggtgagaatc gccggtgaag gtcgtccgtg gaatcccggt
    37561 gccatcaccg gtggccgcgt gtggggggat atcccggaca acagcgtcca gccgggtgcc
    37621 ggaaacggcg aaccggtcac ggcagaggta ctggcacagc ggcaggctga agaggctatc
    37681 cgccgtgaaa cggaacgccg tgcggatgaa attgtccgta aaatggcaga gaacaaacct
    37741 gacctgccgg acggcaaaac agagcaggct gtcagggata ttgccgggct ggagcgtgac
    37801 cggtctgcca tatctgaacg ggaagccgcg ctgccggaga gtgtactgcg tgaaccacaa
    37861 cgggtgcggg aagcggtccg ggaggttgcc cgggaaaatc tgctgcagga gcgactgcag
    37921 cagatggagc gggatatggt tcgtgacctg cagaaagaga aaaccctggg cggagactga
    37981 cacaggaaga taaacgctga tgacaacgga taacacgaac acaacacgta atgattcact
    38041 ggctgcacgg accgatacct ggttgcagtc gttgctggtc tggtcacccg gacagcggga
    38101 tatcatcaaa acggtggcac tggtgctgat ggttctggac catgcaaacc gcattctgca
    38161 tctggaccag tcgtggatgt ttctggccgg gcgaggagcc tttccgctgt ttgccctggt
    38221 gtgggggctg aatctgtccc gtcatgcgca tatccggcaa ccggccatta accggctgtg
    38281 gggatgggga attattgccc agttcgcata ttatctggcc ggatttccct ggtatgaggg
    38341 gaatatcctg tttgcctttg cagtggcggc acaggtgctg acatggtgtg aaacgcgcag
    38401 tgggtggcgt acagcagcgg ccattcttct gatggccctg tgggggcctt tgtccggcac
    38461 cagttacggc attgccgggc tgctgatgct ggcagtcagc caccggctgt accgggcgga
    38521 agacagagcg gaacgtctgg cgctggtcgc ctgcctgctg gctgttattc cggcgcttaa
    38581 ccttgccacc agtgatgcag cggcggtggc cggtcttgta atgacggtgc tgaccgttgg
    38641 tctggtgtcg tgtgcaggaa aatcattacc ccgtttctgg cccggggatt ttttcccggt
    38701 attctacgcc tgtcatctgg ctgtgctggg cgttctggcg ttgtgactgg tatgatactg
    38761 acgctgattc tgattattaa taaacaacat aacacaggat atttatgaaa attacggatc
    38821 acaaactctc tgaaggtatt gccctgacat ttcgtgttcc tgaaggaaat ataaaacatc
    38881 ctttgattat cttatgccat gggttttgcg gtatcaggaa tgttctgctc ccttgctttg
    38941 cgaatgcctt cacagaagca ggattcgcaa cgattacttt tgattatcga ggattcggtg
    39001 agagtgatgg agaacgaggg cgtctggttc ctgcaatgca gaccgaagat attatttcag
    39061 ttatcaattg ggcagaaaag caggaatgta ttgataatca gcgtattggg ttatggggaa
    39121 catcgctggg aggggggcat gtcttcagtg cagctgcgca ggaccaacgc gtaaaatgta
    39181 ttgtcagcca gctggctttt gctgacggtg atgttctggt taccggagag atgaatgaat
    39241 cggagagagc gtcttttctg tccacgctga ataaaatggc agagaagaag aaaaacaccg
    39301 ggaaagagat gtttgtgggg gttacccggg tactgagtga taatgaatca aaggtattct
    39361 tcgagaaggt aaaaggtcag tatcctgaaa tggatataaa aatacctttc cttacggtta
    39421 tggagacgtt gcaatataaa ccggcagaga gcgcagccaa agtacagtgc ccagttttga
    39481 ttgttattgc cggccaggat agtgttaatc caccagaaca gggaaaagcc ttatatgatg
    39541 ctgttgcttc tggtactaag gaattatacg aggaagctga cgcatgccat tatgatattt
    39601 atgaaggcgc gttttttgaa cgtgtggcgg cagtacagac gcaatggttt aaaaaacatc
    39661 tttaattgtc ggtattaata tgtcagaggt cgttgaggtc agcttaatat catagtgagt
    39721 agtttcaacg acctggagga catattttca caaaggtcag ggcaatcatg acagagcaga
    39781 aacgaccggt actgacactg aaacggaaaa cggaagggga aacaccgacc cggagccgga
    39841 aaaccatcat caatgtcacc acgccaccaa aatggaaggt gaaaaagcag aaactggcgg
    39901 agaaggctgc ccgggaagca gagctgacag caaaaaaagc gcaggccaga caggcgctgt
    39961 ccatttatct gaacctgccc tcgctggatg aggccgtgaa caccctgaaa ccctggtggc
    40021 cgggattatt tgacggtgac acaccccgac ttctggcctg cggtatccgg gacgtgttac
    40081 tggaagacgt ggcgcagcgg aatatcccgc tctcgcataa aaaactgcgc agggcgctga
    40141 aggccatcac ccgttcagaa agctatctgt gtgccatgaa agccggtgcc tgccggtatg
    40201 acacggaagg gtatgtgacg gagcatattt ctcaggagga agaagtgtat gcggcagagc
    40261 gtctggataa aatccgccgc cagaaccgga taaaggcaga acttcaggcc gtgcttgatg
    40321 aacaataaaa aagcctcctt cacactaaag gaggcggaaa aataaggctt ctctgaaacc
    40381 ttcttccata cccggcagaa gaaggttata aaaatttata acacattcac cggcctggat
    40441 taaaggggga aggaaaatga acggaacagg gcgtgattcg caaaactccg gggtatggcg
    40501 ctaccggcag gtcggacgcc gggctgtccg gctggaagtc aggggggact ggccgggagc
    40561 cgctgaggca tggctacggg ctgcgcagtc tgctccccgg acagactggc agcagtttgc
    40621 ccgaaaaagg gctgaacact gccaccggcg ctgccgggga agggtgtaac gaaaaagcag
    40681 ccttcattac tccagggatt cagaaggctg aataatgcat gagatttgct ttttttaata
    40741 tccggtcgga aagtatgacc ggacagttct gagtagcaca gagttttttc catattaagt
    40801 cgtgatttta tattttgtga ttaatttcac aaaataaggt gttattcagt gtgtgctgcg
    40861 atattcagga ttacctgaaa tacaggtgcc atttatctga tatggagaat aacatgagga
    40921 aatatattcc actggtatta tttatctttt catggccggt attgtgtgca gatattcatg
    40981 gacgggttgt tcgtgtactg gacggtgata ccatcgaggt catggactca cggaaggcag
    41041 tgcggatcag gctgataaat attgatgcac cggaaaagaa acaggattat ggccggtggt
    41101 caacggacat gatgaaatca ctggttgcag gaaaaacggt taccgtcaca tattttcagc
    41161 gagatcgtta tggtcgtatt ctcgggcagg tttatgcacc agacggaatg aatgttaatc
    41221 agtttatggt ccgggccggg gctgcctggg tatatgagca gtacaacaca gatcccgtgc
    41281 tgcctgtcct tcagaacgaa gccagacaac aaaaacgtgg gctctggtca gatgctgatc
    41341 cggttccccc ctggatatgg agacaccgta aataaagcga tggcaatctt atcattcatc
    41401 tgttctcagc gggaataaaa atggcgaaaa caaaacagga atggctgtat cagttacggc
    41461 gatgttcatc agtcaatacg ctggaaaaaa ttattcataa aaacagagac agtttattaa
    41521 acagtgaacg tgaatcattt aattcagcag cagaccaccg gttagcggaa ttaatcaccg
    41581 gaaagttata tgacaggata ccaaaagaga tatggaaata tgtaaggtaa taattcagca
    41641 taattaatta ccaggagcag ataaacaatg aagctgatta tttttattct tattgttctg
    41701 attattgcag cgttacttat ccggattatc ctcagaagtg taaatcagca ttctcctttg
    41761 ctgatgcaat tgcatgcggc aggtatacgg accggtgatg cagagcgaat actgtccggc
    41821 ggtgaatact ggcaacgtca gaagacgctg ctgacagaaa gggaagtcag ttttatgaaa
    41881 ggactgttca gaattgtgga tatgaagcgg tggtatctgt gtccgcaggt acgggtcgca
    41941 gatatcgtcc agctgaacgg gaatatccgg ccacgatcac gccagtggtg gcagttattc
    42001 aggatggtgt ctcagtggca tgttgatgtg gttatcgttg agcggcgttc gttcagtatt
    42061 gttgcagcag tagagctgga tgatgccagc catttacgac ctgaacgcag acgccgggat
    42121 attcttctgg aagaggttct gaggcaggct ggtattccgt tgctcagaag ccacgatgcc
    42181 agaaaactgc tgcagatgac cggagaatgg ctgaatacaa caggggctgc ccagcagtca
    42241 ccggaacatc gtagctgacg ccttcgcgtt gctcagttgt ccaaccccgg aaacgggaaa
    42301 aagcaagttt tccccgctcc cggcgtttcc ataactgaaa accatactat ttcacagttt
    42361 aaatcacatt aaacgacagt aatccccgtt gatttgtgcg ccaacacaga tcttcgtcac
    42421 aattctcaag tcgctgattt caaaaaactg tagtatcctc tgcgaaacga tccctgtttg
    42481 agtattgagg aggcgagatg tcgcagacag aaaatgcagt gacttcctca tctggcgcaa
    42541 aacgagcata cagaaagggt aaacctttga gtgaagcaga acgacagaga gcagcttctg
    42601 cgcgcaaacg atctgtatgt aaagagatca aggttttcgt cagaccagaa ctgaaaaact
    42661 accttaccag tctgtgtgca gaggaagggg taactcaggc agagttgata gaaaggctta
    42721 tcgagaaaga ggcctgtcac aggaacatga tgtgatggga tcacattctt gctagttttt
    42781 tctgtcagtg ctagattact gatcgtttaa ggaattttgt ggctggccac gccgtaaggt
    42841 ggcagggaac tggttctgat gtggatttac aggagccaga aaagtgaaaa ccccgataat
    42901 cttcagcaag tttggcgact gagaagaaga ttaacggggc taacaagaaa ccgcatagaa
    42961 gctgttgctc tatgcgggga gtatagttat atgcccggaa aagttcaaga cttctttctg
    43021 tgctcactcc ttctgtgcaa cataagtgca ggatggtgtg actgatcttc accaaacgta
    43081 ttaccgccag gtaaagaacc cgaatccggt gtttacaccc cgtaaaggtg ccggaacgct
    43141 gaagttctgc gaaaaactga tggaaaaggc ggtgggcttc acctcccgtt ttgacttcgc
    43201 cattcatgtg gcgcatgccc gttcgcgtgg tctgcgtcga cgcatgccac cggtgctgcg
    43261 tcgacgggca attgatgcgc tgctgcaggg gctgtgtttc cactatgacc cgctggccaa
    43321 ccgcgtccag tgctccatca ccacactggc cattgagtgc ggactggcga cagagtccgg
    43381 tgcaggaaaa ctctccatca cccgtgccac ccgggccctg acgttcctgt cagagctggg
    43441 actgattacc taccagacgg aatatgaccc gcttatcggg tgctacattc cgaccgacat
    43501 cacgttcaca ccggcactgt ttgccgctct cgatgtgtct gaggtggcgg tggcttccgc
    43561 acgccgcagt cgtgtggaat gggaaaacag acagcgcaag aagcagggac tggatgcact
    43621 gggtatggat gaactgatag cgaaagcctg gcgttttgtg cgtgagcgtt tccgcagtta
    43681 ccagacagag cttaagtccc ggggaataaa gcgtgcccgt gcgcgtcgtg atgcgggcag
    43741 ggaacgtcag gatatcgtca ccctggtgaa acgacagctg acgcgtgaaa tctcggaagg
    43801 gcgctttact gctaatggtg agacggtaaa acgcgaagtg gagcgtcgtg tgaaggagcg
    43861 catgattctg tcacgtaacc gcaattacag ccggctggcc acagcttccc cctgaacctg
    43921 acctcctctg aataatccgg cctgcgccgg aggcatccgc acgtctgaag cccgccagcg
    43981 cacaaaaaat cagcaccaca tacaaaaaac aacctcatca tccagcttct ggtgcatccg
    44041 gccccccctg ttttcgatac aaaacacgcc tcacagacga ggaattttgc ttatccacat
    44101 ttaactgcaa gggacttccc cataaggtta caaccgttca tgtcataaag cgccagccgc
    44161 cagtcttaca gggtgcaatg tatcttttaa acacctgttt atatctcctt taaactactt
    44221 aattacattc atttaaaaag aaaacctatt cattgcctgt cctgtggaca gacagatatg
    44281 cacctcccac cgcaagcggc gggccccaac cggagccatt ttagttacaa cactcaaata
    44341 caaccaccaa caaaaccccg gtccagcgca gaaccgaaac cacaaagccc ctctctcata
    44401 attgaaaagc ggccccgccc cggcccaaag ggccggaaca gagtcgcttt taattatgaa
    44461 tgttgtaact atactccatc attgctgtca gtattctggc tggaagtcct cagtacacgc
    44521 tcgtaagcgg ccctcacggc ccgctaacgc ggagatacgc cccgacttcg ggtaaaccct
    44581 cgtcgggacc actccgaccg cgcacagaag ctctgtcatg gctgaaaacg gatatggctt
    44641 agcagggctg gggatgggta aggtgaaatc tatcaatcag taccggctga cgccgggctt
    44701 cggcggtttt actccggtat cacatgtaac cacagcgtgc cgccttccat gccgctggcg
    44761 cggcatatcc tggtgacgat atctgaatcg ttatatactg tgtatatacg tggtaatgac
    44821 gaggtgataa atggcacagg ttaatatgag tttaagaatc gacgctgaac tgaaggatgc
    44881 ttttatggcc gctgcaaaaa gcatggaccg taatggctcc cagttaatcc gggattttat
    44941 gcgccagacc gttgaacggc agcataatac ctggttccgt gaccaggttg cggcaggacg
    45001 tcagcaactc gggcgcggcg atgtacttcc ccatgacatg gtcgaatctt ctgctgctgc
    45061 atggcgggat gaaatgagca ggaaggt
//
LOCUS       DAMZET010000016        44895 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.016, whole genome shotgun sequence.
ACCESSION   DAMZET010000016 DAMZET010000000
VERSION     DAMZET010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.016"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..2971
                     /gene="fdnG"
                     /locus_tag="POB34_001035"
     CDS             <1..2971
                     /gene="fdnG"
                     /locus_tag="POB34_001035"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_except=(pos:506..508,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDI5918025.1"
                     /translation="PSVALAETRQYKLLRTRETRNTCTYCSVGCGLLMYSLGDGAKNA
                     KASIFHIEGDPDHPVNRGALCPKGAGLVDFIHSESRLKFPEYRAPGSDKWQQISWEEA
                     FDRIAKLMKEDRDANYIAQNAEGVTVNRWLSTGMLCASASSNETGYLTQKFSRALGML
                     AVDNQARVUHGPTVASLAPTFGRGAMTNHWVDIKNANLVVVMGGNAAEAHPVGFRWAM
                     EAKIHNGAKLIVIDPRFTRTAAVADYYAPIRSGTDIAFLSGVLLYLLNNEKFNREYTE
                     AYTNASLIVREDYGFEDGLFTGYDAEKRKYDKSSWTYELDENGFAKRDTTLQHPRCVW
                     NLLKQHVSRYTPDVVENICGTPKDAFLKVCEYIAETSAHDKTASFLYALGWTQHSVGA
                     QNIRTMAMIQLLLGNMGMAGGGVNALRGHSNIQGLTDLGLLSQSLPGYMTLPSEKQTD
                     LQTYLTANTPKPLLEGQVNYWGNYPKFFVSMMKAFFGDKATAENSWGFDWLPKWDKGY
                     DVLQYFEMMKEGKVNGYICQGFNPVASFPNKNKVIGCLSKLKFLVTIDPLNTETSNFW
                     QNHGELNEVDSSKIQTEVFRLPSTCFAEENGSIVNSGRWLQWHWKGADAPGIALTDGE
                     ILSGIFLRLRKMYAEQGGANPDQVLNMTWNYAIPHEPKSEEVAMESNGKALADITDPA
                     TGAVIVKKGQQLSSFAQLRDDGTTSCGCWIFAGSWTPEGNQMARRDNADPSGLGNTLG
                     WAWAWPLNRRILYNRASADPQGNPWDPKRQLLKWDGTKWTGWDIPDYSAAPPGSGVGP
                     FIMQQEGMGRLFALDKMAEGPFPEHYEPFETPLGTNPLHPNVISNPAARIFKDDAEAL
                     GKADKFPYVGTTYRLTEHFHYWTKHALLNAILQPEQFVEIGESLANKLGIAQGDTVKV
                     SSNRGYIKAKAVVTKRIRTLKANGKDIDTIGIPIHWGYEGVAKKGFIANTLTPFVGDA
                     NTQTPEFKSFLVNVEKV"
     gene            2984..3886
                     /gene="fdoH"
                     /locus_tag="POB34_001036"
     CDS             2984..3886
                     /gene="fdoH"
                     /locus_tag="POB34_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HDI5918026.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNVGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            3883..4518
                     /gene="fdoI"
                     /locus_tag="POB34_001037"
     CDS             3883..4518
                     /gene="fdoI"
                     /locus_tag="POB34_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDI5918027.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            4515..5444
                     /gene="fdhE"
                     /locus_tag="POB34_001038"
     CDS             4515..5444
                     /gene="fdhE"
                     /locus_tag="POB34_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HDI5918028.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKDPKIEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(5774..6016)
                     /locus_tag="POB34_001039"
     CDS             complement(5774..6016)
                     /locus_tag="POB34_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918029.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(6234..6452)
                     /locus_tag="POB34_001040"
     CDS             complement(6234..6452)
                     /locus_tag="POB34_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDI5918030.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(7309..8250)
                     /gene="fabY"
                     /locus_tag="POB34_001041"
                     /gene_synonym="yiiD"
     CDS             complement(7309..8250)
                     /gene="fabY"
                     /locus_tag="POB34_001041"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HDI5918031.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(8295..8732)
                     /gene="dtd"
                     /locus_tag="POB34_001042"
     CDS             complement(8295..8732)
                     /gene="dtd"
                     /locus_tag="POB34_001042"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDI5918032.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(8729..9601)
                     /locus_tag="POB34_001043"
     CDS             complement(8729..9601)
                     /locus_tag="POB34_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HDI5918033.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(9595..10194)
                     /gene="yihX"
                     /locus_tag="POB34_001044"
     CDS             complement(9595..10194)
                     /gene="yihX"
                     /locus_tag="POB34_001044"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HDI5918034.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(10293..11078)
                     /locus_tag="POB34_001045"
     CDS             complement(10293..11078)
                     /locus_tag="POB34_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDI5918035.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(11112..12008)
                     /gene="yihV"
                     /locus_tag="POB34_001046"
     CDS             complement(11112..12008)
                     /gene="yihV"
                     /locus_tag="POB34_001046"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HDI5918036.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            12176..13072
                     /gene="yihU"
                     /locus_tag="POB34_001047"
     CDS             12176..13072
                     /gene="yihU"
                     /locus_tag="POB34_001047"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HDI5918037.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     SGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            13096..13974
                     /gene="yihT"
                     /locus_tag="POB34_001048"
     CDS             13096..13974
                     /gene="yihT"
                     /locus_tag="POB34_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HDI5918038.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            13991..15232
                     /gene="yihS"
                     /locus_tag="POB34_001049"
     CDS             13991..15232
                     /gene="yihS"
                     /locus_tag="POB34_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HDI5918039.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            15370..16272
                     /locus_tag="POB34_001050"
     CDS             15370..16272
                     /locus_tag="POB34_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HDI5918040.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HTYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKTIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCCDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            16471..18507
                     /gene="yihQ"
                     /locus_tag="POB34_001051"
     CDS             16471..18507
                     /gene="yihQ"
                     /locus_tag="POB34_001051"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HDI5918041.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPAGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVTTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTSVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            18553..19938
                     /locus_tag="POB34_001052"
     CDS             18553..19938
                     /locus_tag="POB34_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5918042.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQHVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            19981..21384
                     /locus_tag="POB34_001053"
     CDS             19981..21384
                     /locus_tag="POB34_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5918043.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            21452..22144
                     /gene="ompL"
                     /locus_tag="POB34_001054"
     CDS             21452..22144
                     /gene="ompL"
                     /locus_tag="POB34_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HDI5918044.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFIRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(22235..23500)
                     /locus_tag="POB34_001055"
     CDS             complement(22235..23500)
                     /locus_tag="POB34_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5918045.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(23602..24582)
                     /locus_tag="POB34_001056"
     CDS             complement(23602..24582)
                     /locus_tag="POB34_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDI5918046.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(24590..25300)
                     /locus_tag="POB34_001057"
     CDS             complement(24590..25300)
                     /locus_tag="POB34_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HDI5918047.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPKTLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(25517..27340)
                     /gene="typA"
                     /locus_tag="POB34_001058"
     CDS             complement(25517..27340)
                     /gene="typA"
                     /locus_tag="POB34_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HDI5918048.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            27713..29122
                     /gene="glnA"
                     /locus_tag="POB34_001059"
     CDS             27713..29122
                     /gene="glnA"
                     /locus_tag="POB34_001059"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HDI5918049.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            29441..30490
                     /gene="glnL"
                     /locus_tag="POB34_001060"
     CDS             29441..30490
                     /gene="glnL"
                     /locus_tag="POB34_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HDI5918050.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            30502..31911
                     /gene="glnG"
                     /locus_tag="POB34_001061"
     CDS             30502..31911
                     /gene="glnG"
                     /locus_tag="POB34_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HDI5918051.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            32023..32133
                     /locus_tag="POB34_001062"
     CDS             32023..32133
                     /locus_tag="POB34_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HDI5918052.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(32323..33696)
                     /gene="hemN"
                     /locus_tag="POB34_001063"
     CDS             complement(32323..33696)
                     /gene="hemN"
                     /locus_tag="POB34_001063"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDI5918053.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(33885..34394)
                     /gene="yihI"
                     /locus_tag="POB34_001064"
     CDS             complement(33885..34394)
                     /gene="yihI"
                     /locus_tag="POB34_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HDI5918054.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVIEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            34976..35608
                     /locus_tag="POB34_001065"
     CDS             34976..35608
                     /locus_tag="POB34_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDI5918055.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(35990..38776)
                     /gene="polA"
                     /locus_tag="POB34_001066"
     CDS             complement(35990..38776)
                     /gene="polA"
                     /locus_tag="POB34_001066"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDI5918056.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPNDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            39140..40072
                     /locus_tag="POB34_001067"
     CDS             39140..40072
                     /locus_tag="POB34_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDI5918057.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(40113..41543)
                     /locus_tag="POB34_001068"
     CDS             complement(40113..41543)
                     /locus_tag="POB34_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDI5918058.1"
                     /translation="MIHKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVKWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVATETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(41698..42324)
                     /gene="dsbA"
                     /locus_tag="POB34_001069"
     CDS             complement(41698..42324)
                     /gene="dsbA"
                     /locus_tag="POB34_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HDI5918059.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(42341..43327)
                     /gene="srkA"
                     /locus_tag="POB34_001070"
     CDS             complement(42341..43327)
                     /gene="srkA"
                     /locus_tag="POB34_001070"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HDI5918060.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEHGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLMPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(43404..43673)
                     /locus_tag="POB34_001071"
     CDS             complement(43404..43673)
                     /locus_tag="POB34_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HDI5918061.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            43743..44327
                     /gene="mobA"
                     /locus_tag="POB34_001072"
     CDS             43743..44327
                     /gene="mobA"
                     /locus_tag="POB34_001072"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HDI5918062.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPPDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMVF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            44309..44836
                     /gene="mobB"
                     /locus_tag="POB34_001073"
     CDS             44309..44836
                     /gene="mobB"
                     /locus_tag="POB34_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDI5918063.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT        11068 a        11434 c        11046 g        11347 t
ORIGIN      
        1 acccagcgta gcgctcgcgg aaacccggca gtataaactg ctgcgcaccc gcgaaacccg
       61 taatacctgc acctattgtt ccgtaggctg tgggctgttg atgtacagcc tcggtgacgg
      121 agcaaaaaac gccaaagcat ctatcttcca tatcgaaggc gatccggatc acccggtcaa
      181 ccgcggtgca ctttgtccga aaggcgctgg cctggtggat ttcatccact ccgaaagccg
      241 tctgaagttt ccggaatacc gtgcgccagg ttctgataaa tggcagcaaa tcagttggga
      301 agaggcgttt gatcgcatcg ccaaactgat gaaagaagac cgcgatgcta actacattgc
      361 gcaaaacgcc gaaggcgtga ctgttaaccg ctggctctcc accgggatgc tgtgtgcttc
      421 cgcgtcgagt aacgaaaccg gctatttaac gcaaaaattc tcccgcgcgc tgggtatgct
      481 cgcggtcgac aaccaggcgc gtgtctgaca cggaccaacg gtagcaagtc ttgctccaac
      541 atttggtcgc ggtgcgatga ccaaccactg ggtcgacatc aagaacgcca acctcgtcgt
      601 ggtgatgggc ggtaacgccg ctgaagctca cccggtcggg ttccgctggg cgatggaagc
      661 caaaattcac aacggcgcga agctgattgt gatcgatcct cgctttacgc gtacggcggc
      721 ggtagctgac tactatgccc ctattcgttc cggtactgac attgctttcc tgtcaggcgt
      781 attgctgtac ctgctgaaca atgaaaaatt caaccgcgaa tacaccgaag cctataccaa
      841 cgcaagcctg atagtgcgtg aggattacgg ctttgaagat ggcctgttca ccggctacga
      901 cgcggaaaaa cgcaagtacg ataaatcctc ctggacttat gaactggacg aaaacggctt
      961 cgccaaacgc gataccacgc tgcaacatcc gcgctgcgtg tggaacttgc tgaaacagca
     1021 cgtttcccgc tacacgccag atgtggttga aaacatctgt ggtacgccaa aagacgcgtt
     1081 cctgaaagtc tgcgaataca tcgcagaaac cagtgctcac gataaaactg cctcgttcct
     1141 gtatgccctc ggctggacgc aacactccgt tggtgcgcaa aacattcgta cgatggcgat
     1201 gatccagctg ctgctcggca atatggggat ggccggcggc ggcgttaacg ccctgcgcgg
     1261 tcactccaat attcaggggc tgacggacct ggggctgctg tcgcagagtc tgccaggtta
     1321 catgacgctg ccaagcgaga agcagaccga tctgcaaacc taccttaccg ccaacacgcc
     1381 aaaaccgctg ctggaaggcc aggtaaacta ctggggcaac tacccgaaat tcttcgtctc
     1441 tatgatgaag gccttctttg gtgataaagc gacggcggaa aatagctggg gctttgactg
     1501 gttgccgaag tgggataaag gctacgacgt cctgcaatac ttcgagatga tgaaagaggg
     1561 caaggtcaat ggctatatct gccagggctt taaccctgtt gcctcattcc cgaacaaaaa
     1621 caaagtgatc ggttgtctgt cgaaactgaa gttcctcgtc accatcgacc cactgaacac
     1681 tgaaacctct aacttctggc agaaccacgg tgagctgaac gaagttgact cgtcgaagat
     1741 ccagaccgaa gtgttccgtc tgccatcgac ctgcttcgca gaagagaacg gttcaatcgt
     1801 taactcaggt cgctggttgc agtggcactg gaaaggtgcg gacgccccgg ggattgcgct
     1861 gactgacggt gagatcctct ccggtatctt cctgcgcttg cgcaagatgt atgccgaaca
     1921 gggtggcgcg aacccggatc aggtgctgaa catgacctgg aactacgcca ttccgcatga
     1981 gccgaagtcg gaagaagtag cgatggagag caacggtaag gcgctggccg atattaccga
     2041 tccggcaacc ggtgcggtta tcgtcaagaa aggccaacaa cttagctcgt tcgcccaatt
     2101 gcgcgatgac ggtacaacct cctgtggctg ctggattttc gccggtagct ggacgccgga
     2161 aggcaaccag atggcgcgtc gtgataacgc cgatccgtct ggcctcggta acacgctggg
     2221 ctgggcatgg gcatggccgc ttaaccgccg cattctgtat aaccgcgcct ccgcagatcc
     2281 gcagggtaac ccgtgggatc cgaagcgtca gttgctgaaa tgggacggca ctaagtggac
     2341 cggctgggat attccggact acagcgcagc gcctccgggt agcggcgtcg ggccgtttat
     2401 catgcagcag gaaggcatgg ggcgtctgtt tgccctcgat aagatggcgg aaggtccgtt
     2461 cccggaacac tacgagccgt ttgaaacgcc gctgggaact aacccgctgc atccaaacgt
     2521 tatctcgaac ccggctgcac gtatctttaa agacgacgcc gaagcattgg gtaaagccga
     2581 taagttcccg tatgtcggaa ccacctatcg tctgaccgag cacttccact actggaccaa
     2641 acacgcgctg ttgaacgcga ttttgcagcc agagcagttt gtggaaatcg gtgagtcgct
     2701 ggcgaataaa cttggcattg cccagggcga taccgtgaaa gtctcctcca accgtggcta
     2761 tatcaaagcc aaagcggtgg tgaccaaacg tattcgcacg ctgaaagcga acggcaaaga
     2821 tatcgatacc atcggtattc cgattcactg gggctatgaa ggcgtagcta aaaaaggctt
     2881 tattgccaat acgttaacgc cattcgttgg tgatgcgaac acgcagacgc cggagtttaa
     2941 gtccttcctt gtgaatgtgg aaaaggtgta acggagacga catatggctt atcaatctca
     3001 agatatcatt cgtcgttccg cgactaacgg tctgaccccc gcgcctcaag cgcgggactt
     3061 ccaggaagaa gtggcgaaac tcatcgacgt caccacctgt atcggctgta aagcctgtca
     3121 ggtggcgtgt tcagagtgga acgacattcg cgataccgtc ggcaataacg ttggggtgta
     3181 cgacaacccc aatgatttaa gcgccaaatc gtggacggta atgcgcttct cggaagtgga
     3241 gcagaacgac aaactggaat ggctgatccg caaagacggc tgtatgcact gttccgatcc
     3301 aggctgcctg aaagcgtgcc cggcggaagg tgcaattatt cagtatgcca acggtatcgt
     3361 cgacttccag tccgagcagt gcattggctg cggttattgc attgcgggct gtccgttcga
     3421 cattccgcgc ctcaacccgg aagacaaccg cgtctacaaa tgtacgctgt gcgttgaccg
     3481 cgtggtggtt gggcaagaac cagcctgcgt gaagacctgc ccaacgggcg cgattcactt
     3541 cggtacgaaa gagtcgatga aaacgctggc gagcgagcgc gttgctgagc tgaaaacccg
     3601 cggttacgac aatgcgggtc tgtacgatcc ggcaggcgtc ggtggtacac acgtcatgta
     3661 cgtgctgcac catgctgaca agccaaatct gtatcatggc ttgccggaga acccggaaat
     3721 cagcgaaacc gtgaaattct ggaaaggcat ctggaaaccg ctcgcggctg ttggctttgc
     3781 ggctaccttt gcggccagta tcttccacta cgtgggtgtc ggtccgaacc gtgcggatga
     3841 ggaagagaat aatctgcacg aagagaaaga cgaggagcgc aaatgaaacg acgtgacacc
     3901 atcgtgcgct acaccgcgcc ggaacgtatc aaccactgga tcaccgcctt ctgcttcatc
     3961 ctggcggcgg tgagcgggct gggctttttg ttcccgtcct tcaactggtt gatgcaaatc
     4021 atgggcacac cgcagctggc gcgaattctg cacccgtttg tcggcgtggt tatgtttgcc
     4081 tcgttcatca tcatgttttt ccgttactgg catcacaacc taatcaatcg ggatgatatc
     4141 ttttgggcga agaatattcg taagatcgtc gtcaacgagg aagtaggtga caccgggcgt
     4201 tataacttcg gtcagaaatg cgttttctgg gcggcgatta ttttcctggt tctgctgctg
     4261 gtgagcggcg tgattatctg gcgtccttat tttgcgcctg ctttctcaat cccggtgatc
     4321 cgattcgcgt taatgctgca ttcatttgcc gcagtggcgt taattgtggt tatcatggtg
     4381 catatctacg ccgccctttg ggtgaaaggc acgattaccg ccatggtgga aggatgggtt
     4441 accagcgcct gggcgaagaa acatcacccg cgctggtacc gtgaggtccg caagacaacg
     4501 gaaaagaaag ctgaatgagt attcgcataa tcccgcaaga tgagctgggt tcaagcgaga
     4561 aacgtacggc ggatatgatt ccgccgttat tgttccctcg gctcaagaat ttatacaacc
     4621 gccgcgccga gcgtctgcgc gagctggcag aaaataatcc tctgggtgat tacctgcgct
     4681 ttgctgcgct tatcgcccac gcccaggaag tggtgctgta cgaccatccg ctggagatgg
     4741 atctgactgc gcgcattaaa gaagccagcg cacaaggcaa gcccccgctg gatattcacg
     4801 ttctgccgcg tgataagcac tggcaaaagc tgctgatggc gctgattgct gagctgaaac
     4861 ctgaaatgag cggcccggcg ctggcagtga ttgagaatct ggagaaggca tcgactcagg
     4921 agctggaaga tatggccagc gcgctgtttg cctctgattt ctcgtccgtc agcagcgata
     4981 aagcgccgtt tatctgggct gcactgtcgc tctactgggc gcagatggcc aatctgatcc
     5041 ccggcaaagc ccgcgctgaa tacggcgagc aacgtcaata ttgcccggtt tgtggttcta
     5101 tgccggtgtc cagcatggtg caaattggca ccactcaggg gctacgttac ctgcactgca
     5161 acctgtgtga aactgaatgg cacgtagtgc gcgtaaaatg cagcaactgc gagcagagcg
     5221 gcaaactgca ttactggtcg ctggatgacg aacaggccgc gattaaagcc gaaagctgcg
     5281 atgactgcgg cacttacctg aaaattctct atcaggaaaa agatccaaaa atcgaagccg
     5341 tagcagatga cctcgcctct ctggtactgg acgcacgaat ggagcaagaa ggctatgccc
     5401 gcagttccat caacccgttc ctgtttccgg gtgaaggaga gtaatctctg acgttttccg
     5461 ggtggcgcag gtcgcccgga ttacagctca tagggtgtga cgacaccatc tctcgtattc
     5521 cgcgttacca cctcaagcta tgccgccagt gccggtttgg cctcaattta cccaccacca
     5581 catcaataag gttatgggca tgagactgat gacaagcgta agattgcctg atgcgctgcg
     5641 cttatcaggc ctaccaataa actgcaatac attgaattta tacgactttg taggccggat
     5701 aaggcgttca cgccgcatcc ggcatgaaca acgcgcacgt tgtcagcaat ctgattgaca
     5761 tcattttttc ccattaccat tcctcgcgca gcttacgctg atattcgaca ccatcttcct
     5821 cacacccttg ccagatgcca ggaacactgg ttcttgctgt ttgcgattga tttatcaggt
     5881 attgatctac cgcttccctt aataggtccg cacgaggaag attacgctgc acctcgagat
     5941 catcaagttg cttaatcacc tcattcgata aatcgagtaa aattctgccc atatctatac
     6001 ctgccagaga gttcataaat atctcgccaa tatcattttt gaatctatgg agagaaaagt
     6061 acccttgccg aatctttaaa gaaagctcat ttacgcatca ttttttattt ttgcgaggcg
     6121 ttttccagat atgatgtaac agttgcagtt attataagtt caagtaaaac agccttgtat
     6181 taataccaga ataagggcat ctgtttttta tattcaagaa tgaaaaattt ttgtcattcc
     6241 ttatgctcct tacactgatc atcaacacca actacctccc ccttttgcct gcctcccttt
     6301 gctttacgca ccgggaattg ctgacgttcc agatattgct ctaccgcttc gcgtaaaatt
     6361 gcagatcgtg aaaccttacg ccagccccga agcttgttca gccgtttaat tgcctcttct
     6421 gataaatgaa gaaaaaccgt attcatcgcc attaccacca ccttacctat attactcatt
     6481 aatgtatgtg cgaaaattac gccggaaaat catttaaaaa aagcggattt ttgcatgttg
     6541 ttttgttttt gcgaggcgtt ttccagaaat aataagagtg ttgcagttgt ttcatttatt
     6601 actcaaaaat ggtttagtta tcgcccagga tgaattatgg gtgctatgaa tatattatat
     6661 tcagagatat ttatttgcat aatctgaatg gtgtagccga aaaacgtcag catcctaaca
     6721 gcaccaggat ttaaggtgaa attaatcttt catcacagac cggtcagtac tgccaccaac
     6781 cggtctttac ttgctcatcg ttataatttt gttggtaaca gttcgggaaa gttgtttttc
     6841 taaattaaca acagaataac tttcttgttt aatgagatta atattggcaa ccattatagc
     6901 ctccttccca cggtttaaga gcaaacatac aacataatta ttcataacat acagagcagt
     6961 aaatgaatga tactgacaat gatgctagtc cattatcttt gcataatatt tgttagctat
     7021 agattttaaa aatatttatg agaaaagtat ggcaccagag ggggttttga ctatcaaaaa
     7081 tcgctcaaac ctgaactaag atgatgagtt aagcattatc tgcgataatt gccactcagc
     7141 tctacaaata atatttaaat gctcctgaac agtgatgtag caagacacgt atggagccaa
     7201 cgaagaataa aaatttatag agttcttatt caatataacc ggaatagtca ggggctgatg
     7261 taaatgaccg gaatgagtaa gagaggcagc accgcccctc tccatcagct actcttcttc
     7321 gttcccgccc tcttcatacg ggccaaatgg cttcgcgggc agaacgatat acgtgccttc
     7381 aaacactgca cccggcgtct cgtcgccaaa gatttcaact tgcatctgca cccgtgcttt
     7441 tcgtccgcgc gccagacggt cgagatcgcc gcttaaggca ccaaggtcgg ctaccgcatg
     7501 aggtttaccg ctaatcggct tgctgtagcg gatatgcgca tccgccagaa taatcgttcc
     7561 gccgagatgg cgttcgcgca gcatcagcca gataagcccc caaccggtga gcgttgccag
     7621 tgagaataaa ctcccggcaa acagcgtatg gtgcggattc tgattaccgg tttccggcat
     7681 ggtggtgata aatttttgcc cggtatattg ttgaatacgc acgcccattt tttcactaag
     7741 cgggatatgt tcgtaccacg cctgttgcag ctgcgcgcac cagtcgccgc gatgcaaaat
     7801 atcatccaga gtggcaacgg gtttaatcat caaaaaatgg cgaatcggcg tggtagttgg
     7861 ggtggtgatc tccccctgat taatgaatcc cagcttggcg aaaaactcca ccgcgtcttc
     7921 acgggcgcta caggtcacgc gctttacgcc ttcctgacgc gccaccgact ccagggtcat
     7981 cgccatcagc gtgcctaacc ctttgtcctg cacgtccggg tgaacggcca taaagcgaat
     8041 ggacgcttca ttgtcggcat taatatacaa tcggcctacc gccaccagat taccctgctc
     8101 gtcgacgacc atctggtgat gcgccatcgc atcccacgcg tcgcgttccg aaccttttgg
     8161 ttgatgcagg ggcttacgca acatttccca gcgaaactga tagtaacgct ctaattcttc
     8221 ttctgtttgt ggaacccgaa ggtgatacat agcgatactc tctcttgttt cccgtgacaa
     8281 ccctggaagc tggctcatac ctgcaaccag aatgtcacgg ggccatcatt gaccagcgat
     8341 acctgcatat ccgcagcgaa gcgtcctgtt tgcgtgttca tctcttgctg acggcagcgt
     8401 tcgacgaaat agtcatataa cgcctctgcg cgatccggtg atgcaccttt ggagaaactt
     8461 gggcgcatcc cccgttcggt atctgcggcg agggtaaact gggaaaccac cagcacactg
     8521 ccgcccgcct gttgcacgtt gaggttcatc ttgccttcgg catcgctaaa gatgcggtag
     8581 ccgagcacac gctcgcacag acggtttgct ttctgttcgt cgtcatcctt ttcgacaccc
     8641 aataacacca aaagtcccgc gccaatttcg cccgtcactt ctccctccac ggtgacgctg
     8701 gcacgggtta cgcgttgaat taatgcaatc atggttcgtc gtcttcttct tgttcagctg
     8761 cttgttttag tttgcggtat tccccgagag tgacagtaat ttccgcgcca agcaagacga
     8821 tacaccatgt ccagtagacc caaacaaaga gaatggggat caccgccaac acgccgtaaa
     8881 tgagctgata tgacggaaac atggtgatat aaagcgcgaa acctttcttt cctgcttcga
     8941 acaggagcgc ggcgacaaac gcgccgacaa tcgcgtcgcg gttaggtacg cggatggtag
     9001 gaacaatgct gtacagcaac cagaaggaga tccacgacaa cagcagcgga aaaatacgca
     9061 gcacgttatc gatgacagta ttgagatcgc tcgcccagcg cagggagagc aaataggaac
     9121 tgatcgccag actggcccct gccagcagcg gccccagcgt taaaatcatc cagtacacgg
     9181 cgaacgagta aatttttggt cgcgctcgtt tactgcgcca gatggtattc aacgcgctat
     9241 cgatggagta catcagcaat aacgcggtga cgatcagccc gcacgcccca acggcggtca
     9301 tcttgttgga attggcaaca aattgttcga tataccgctg aataacatcg ccagtagcag
     9361 gcagaaagtt ggcaaaaata aagtgacgta actgaatgct gacgtcggaa aacatgggaa
     9421 aagcggcgaa aagcgcaaaa acaacggcaa ccagcggcac taatgagagc aacgacacat
     9481 aggcaaggtt acctgccagg gttgtcatgt tgtcctcatc aatgcgttgc cagagtagtt
     9541 ttagccaggc ccatagtgga cgggtacggt gcctggcttt gtcctgaatg gtttttagca
     9601 taacaccttc gcgaaatagt ccgggatggt ggttttatct ttcaccagaa tactggtaat
     9661 gcccagctga ttggctcctt ctatattatc ggcgttatcg tcgaaaaaga ccgtatcgct
     9721 gggtgaaaaa ccttccgcct gcaaaacatg ctggtaaatt cgtgcttcag gtttgcgcat
     9781 ccccagatct tgcgacagat agatatggtc agcagcatca cgaatttccg ggtattcttc
     9841 cggccagaag gtggtatgca ggcggttggt attggaaagc accaccacgc gatgcccctg
     9901 ctcacgcagt ttatgcatga tggcgatcac ttccgggcgc agcgccacaa acaccgcctg
     9961 ccagccgtga gagaactgct cgtagcttag cggtagagcc atctcatgac acagcgcctc
    10021 tgcgaacgct tcgtcgctaa tttccccacg ctcatgctga tgaaacgcct cccccatatg
    10081 aaaactcttc ttaagcgatg ccagcggaat acgcgttaaa tcgctccagg ctcccagcac
    10141 acggttaaag tcgatatcga caatcacatt acctaaatca aagatataga gcatttttgc
    10201 ctcctttcgc gccacaagaa attaactgta gcgggaaagg atgggtttgg ctatgtggca
    10261 aggtatgaag ccaggttaac ggtcacaaaa atctacgcgt cttcctgggg aagaatgatt
    10321 tcaatttggc tgtcttgtaa gcgtgatttt agcgcagcgg gcggcagctc gtcggtaaag
    10381 agcgcagtga cctgtgccac gttaccaatt tcaaccgccg ccgaagcatg atacttagtg
    10441 tgatcggcga ccagcagaat atttctcgcg tgcgccatca tcgttttcac cacgttagct
    10501 tcgtttacat caaactccat caacgcgcca tcgctctcaa tcgccccaac gcttgttacc
    10561 agataatcag cacgaaaatc agccacaaag gacgccgctg aagggccaat gatcccgcta
    10621 ttatgagagc gcaacgtacc gccgggcacc atcacttcaa agcgcgggtt gtggtaaaga
    10681 atatgcgcca cacgcaggct gttggtgatt atccgcaaat gattatggtt aagtaacgcc
    10741 cgggcaacat gctcaacagt cgtaccaatg gtgataaata ttgttgaacc atcaggaata
    10801 tagtctgcca ccgcttcggc aatcgctttt ttttcctcgg tttgcgaaac ctcacgctgc
    10861 tcgaacgccg tattaacgac gctggaagcc cgacccgcgc caccgtgatg gcgcgtaatc
    10921 aggccttgct cgcttaattt acgaatatcc cggcggaccg tctgcgtgga gacatccagc
    10981 agatttgcca gctcatcaat attcatatac ccacgctcgg cgatcagcat gaggagttgg
    11041 tcgtgccgcg ggttaccggt tagttcggta aggctcatga aaattcctcg aaaaaccatc
    11101 accctggcat tttatacaaa aagtgacaaa aaagatcggg tttgatcaca gtcagggatc
    11161 ccggctcgtc caccgggacg tgtgcatttt aacgccgcta caccgctggc gaagcggact
    11221 gactccgcta aatccccact tgttgccagc gccaccgcca aagcgccgtg aaaaacatca
    11281 cccgcacctg tggtatctac cacatcaact ttgaaggccg gttgatgctg acgcccacca
    11341 ttttccagcc agtcgcagcc tgcgctaccc tgggtcacat agacatgacc atttgtgagc
    11401 gtttgtgcct gttttagcgc actggccatc tctttcacgc ccgttaagcg cgccagaccc
    11461 ggttctgaaa aggccgcgtg atcgcttaat gccaccagct cactgatatc ctgcggcgta
    11521 atgtccccgt ccagaacggt catcacaccc gcctgacggg ccagggtgaa ggctttttta
    11581 gcgccgtcgt gccagcgtac atctgccagc acaacatccc actgagagaa atcaatttcc
    11641 tccaaccact ctgcgtcagg cagcagatcc gggctggggt agttaatgat tatccgctcg
    11701 cctttggtat ccaccatgat ggcggattgc gaagatttcg cctggttata ccgtttggtg
    11761 taacgggtgt taacgcccca ggattccagc tctgccagca ggctgttgcc cgtgtcgtca
    11821 tcacctacgc gaccaataaa atcgacctgc gcccccagcc ttgccgccgc aaccgctgcc
    11881 gtcgccgctg gcccgccacc aacttccgta taatttctcg ccacgtattt accgctctcc
    11941 gtcggtaacc cttccacgta atagatgcga tccatcacgg ttatacctac acaagcaaca
    12001 cgaatcatgg tcattcctta aacattttgg gatgcgttca ttttaatttc accaattgtt
    12061 taaaaagtga cggctgtcaa ttttttgacg ataaattaca aatacgatca aaaacagaca
    12121 agcaaagcat gtcataaatg acaaaaagtg acatcactgt attcaggaga gggttatggc
    12181 agcaatcgcg tttatcggtt taggacaaat gggttcgcca atggcgagca atttattgca
    12241 gcaagggcat caacttcgcg tctttgatgt gaacgccgaa gctgtgcggc atctggtaga
    12301 caaaggtgcg actcccgccg ccaacccggc gcaggccgct aaagatgccg aatttatcat
    12361 taccatgttg ccgaatggcg atctggtgcg caacgtgttg ttcggtgaaa acggcgtttg
    12421 cgaaggctta tctaccgatg cgctggtcat tgatatgtcc accatccatc cgctgcaaac
    12481 cgataaattg attgccgata tgcaagccaa aggcttcagc atgatggatg ttccggtagg
    12541 ccgtacttct gcaaatgcca ttaccggtac tctgttactg ctggctggcg gcaccgctga
    12601 acaagttgaa cgtgccacac cgatcctgat ggcgatgggc agtgagttga tcaactctgg
    12661 cggtccgggc atggggatcc gcgttaagct catcaacaac tacatgagca tcgcgctcaa
    12721 tgcgctttcg gcagaagccg ccgttttgtg cgaagccctg aatcttccct tcgatgttgc
    12781 cgtcaaagtg atgagcggta ccgccgccgg taaaggtcac ttcaccactt cctggccaaa
    12841 caaagtcctc agcggagatc tttctcccgc cttcatgatc gatcttgccc ataaggatct
    12901 tggcatcgcc cttgatgtcg ccaaccagct gcatgtgcca atgccgctgg gggccgcctc
    12961 acgggaggtt tatagccagg cgcgcgcagc gggtcgcggt cgccaggact ggtccgccat
    13021 tctggaacag gtccgtgtca gtgccgggat gactgccaaa gtaaaaatgt aacgactgga
    13081 taaaggaata aatgaatgaa taagtacacc atcaacgaca ttacgcgcgc atcgggcggt
    13141 tttgccatgc tggcggtcga tcagcgcgaa gccatgcgca tgatgtttgc cgcggctggg
    13201 gcacccgctc cggtagccga tagcgtttta actgatttca aagttaacgc tgcaaaggcc
    13261 ctctcgcctt atgcctcggc gattctggta gatcaacaat tctgctaccg ccaggtggtt
    13321 gagcaaaacg cgattgccaa aagttgcgcc atgattgtcg ccgccgatga gttcattcct
    13381 ggcaacggta ttccggtcga tagcgtggtt attgaccgca aaatcaatcc gctacagatc
    13441 aaacaagacg gtggcaaagc cttaaaactg ctggtgctgt ggcgtagtga tgaagatgcg
    13501 cagcaacgtc tggatatggt gaaagagttc aacgaactgt gccactcaca cggtctggta
    13561 agcatcattg agccagtcgt ccgcccaccg cgtcgtggcg ataaattcga tcgcgaacaa
    13621 gcgatcatcg atgccgccaa agagctgggc gacagtggcg ctgacctcta caaagttgaa
    13681 atgccgcttt atggcaaagg tccgcaacaa gagcttctct gtgcttcaca acgtctgaat
    13741 gaccatatca atatgccatg ggtgatcctc tcttccggtg tcgacgaaaa actgttcccg
    13801 cgtgccgtac gcgtggcaat gacggcgggc gcatcgggat tcctggcagg ccgtgcagtc
    13861 tgggcatcgg tcgtcggttt accagacaac gagctgatgc tgcgtgacgt ttgcgcaccg
    13921 aaattacaac aacttggcga tatcgtcgac gaaatgatgg ctaaacgccg ctaagatgag
    13981 gataaaagga atgaaatggt ttaacaccct aagccacaac cgttggctgg aacaggaaac
    14041 cgaccgcatc tttgattttg gtaaaaattc cgtagtgccg actggttttg gctggttagg
    14101 caataaaggg caaatcaaag aagagatggg cacccatctg tggatcaccg ctcgtatgtt
    14161 gcacgtttat tccgttgctg cggcgatggg tcgacctggc gcttactcgt tggttgatca
    14221 cggtatcaaa gccatgaacg gcgcactgcg tgataaaaaa tatggcggct ggtatgcctg
    14281 cgtgaatgac gagggcgtgg tggatgcctc caaacagggc tatcaacact tctttgctct
    14341 gctgggtgcc gccagcgccg tcacaacggg tcacccggaa gcgcgcaagc tgctcgatta
    14401 caccattgaa attatcgaga aatatttctg gagcgaagaa gagcagatgt gcctggaatc
    14461 ctgggacgaa gccttcagca aaaccgaaga gtaccgcggc ggcaatgcca atatgcacgc
    14521 ggtggaagct ttcttgattg tttatgacgt cactcacgac aaaaaatggc tggatcgcgc
    14581 gattcgcgtg gcttccgtga ttatccacga cgtcgccaga aataatcatt atcgcgttaa
    14641 cgaacacttc gatacccagt ggaatccgct gccggattac aacaaagata acccggcgca
    14701 tcgcttccgc gcgtttggag gtacaccagg ccactggatc gaatggggcc gtttaatgct
    14761 gcacatccat gctgccctgg aagcccgttg cgaacaacca ccagcatggc tgctagaaga
    14821 tgccaaaggt ctgtttaacg ccaccgtgcg cgatgcctgg gcacccgatg gcgcggacgg
    14881 gattgtttat accgttgact gggaaggaaa accggtggtc cgcgaacgtg tacgttggcc
    14941 tatcgtcgaa gcaatgggta cggcctacgc gctctacacc gtcactggcg atcgccagta
    15001 tgaaacctgg tatcaaacat ggtgggagta ctgcattaag tacctgatgg actatgaaaa
    15061 tggttcctgg tggcaggagc tggatgcgga caataaggtc accaccaaag tctgggacgg
    15121 caaacaggat atttatcacc tgctgcattg cctggtgatc ccgcgtatcc cgttagcgcc
    15181 cggcatggct ccagcggttg cagcgggtct gctggatatt aatgcgaaat aactgatctg
    15241 aaaatagggt gcgggttttc ccctcaccct aaccctctcc ccaaaggggc gaggggaccg
    15301 tccggtgcag tttttgggtt tgacatcagc ctcacgggag gctgtatgtc gttaattaag
    15361 gtgccttgta tgcaaatcac caatatgcat tgcagtggac agaccgtttc acttgccgcg
    15421 ggggattatc acgcgaccat tgtgaccgtc ggtgctggtc tggctgaatt aacctttcag
    15481 ggttgtcatt tagtcatccc gcacaaaccc gaagagatgc cgctggcgca tctgggtaaa
    15541 gtgcttattc cgtggccgaa ccggatcgcc aacggctgtt accgctatca ggggcaggaa
    15601 tatcagttac ccattaatga acatagttca aaagccgcta ttcacgggct gcttgcctgg
    15661 cgagactggc aaattagtga attaaccgcc accagcgtta ccctgacagc ttttctgccg
    15721 cccagttacg gctatccgtt tatgctggct tcacaagttg tttattcgct caacgcacac
    15781 acgggtcttt ccgttgagat cgcaagccag aatatcggca ctgtcgcagc gccttatggc
    15841 gtgggtattc atacttattt aacctgcaac ttaacatctg ttgatgaata tctgttccag
    15901 cttccggcaa accaggttta tgccgttgat gaacacgtaa atccgacaac gcttcaccac
    15961 gttgatgagc tggatttaaa tttcacgcag gcgaaaacaa tagccgcgac aaaaatcgac
    16021 cacaccttta aaaccgctaa tgatctgtgg gaaataacca ttacccatcc acaacaggcg
    16081 ctttcggtta gcctttgttg cgatcaaccc tgggtacaaa tttatagcgg tgaaaaatta
    16141 cagcgtcagg gactggcagt tgaacctatg agttgcccgc ctaacgcctt taattcaggt
    16201 atcgacttat tattactgga accaggaaaa ccgcaccgcc tgttttttaa catttacgga
    16261 cagagaaaat aacttgtatt gattagcagt caaaatcatt gaagaacatt tcactcgcta
    16321 aaggcaacat tatgtatctt caatttatta agttaaaaac agctcagctt ctgtaaagtt
    16381 tttatatatc agttttatga gaatcatttt acataagcct gtaataacag gaaggcaaca
    16441 atctcgttct caactctatg gagattagtt atggatacgc cacgtccaca gttattagat
    16501 tttcaatttc atcagaataa cgacagtttt accctacatt ttcaacaacg tcttatttta
    16561 acccatagca aagataatcc ttgtttatgg attggctcag gtatagcgga tatcgatatg
    16621 ttccgcggta atttcagcat taaagataaa ctacaggaga aaattgcgct taccgacgcc
    16681 atcgtcagcc agtcaccggc tggttggtta attcatttca gccgtggttc tgacattagc
    16741 gccacgctga atatctctgc cgacgatcag ggccgtttat tgctggaact acaaaacgac
    16801 aaccttaacc acaaccgtat ctggctgcgc cttgccgctc aaccagagga ccatatctac
    16861 ggctgcggcg aacagttttc ctacttcgat ctgcgtggca aaccgttccc gctatggacc
    16921 agtgaacaag gcgttggtcg caacaaacaa acctatgtca cctggcaggc cgactgcaaa
    16981 gaaaatgcgg gcggcgacta ttactggact ttcttcccac agcctacgtt tgtcaccacg
    17041 cagaagtatt actgccatgt tgataacagt tgctatatga acttcgactt tagtgccccg
    17101 gaataccatg aactggcgct gtgggaagac aaagcaacgc tgcgttttga atgtgctgac
    17161 acatacattt ccctgctgga aaaattaacc gccctgctgg gacgccagcc agaactgccc
    17221 gactggattt atgacggagt aacgctcggc attcagggcg ggacggaagt gtgccagaag
    17281 aaactggaca ccatgcgtaa cgcgggcgtg aaggtcaacg gcatctgggc gcaggactgg
    17341 tccggtattc gtatgacctc ttttggcaaa cgcgtgatgt ggaactggaa gtggaacagc
    17401 gaaaactacc cgcaactgga ttcacgcatt aagcagtgga atcaggaggg cgtgcagttc
    17461 ctggcctata tcaacccgta tgttgccagc gataaagatc tctgcgaaga agcggcacaa
    17521 cacggctatc tggcaaaaga tgcctctggc ggtgactatc tggtggagtt tggcgagttt
    17581 tacggcggcg ttgtcgatct cactaatcca gaagcctacg cctggttcaa ggaagtgatc
    17641 aaaaagaaca tgattgaact cggctgcggc ggctggatgg ctgacttcgg cgagtatctg
    17701 cccaccgaca cttacctgca taacggcatc agcgccgaaa ttatgcataa cgcctggccc
    17761 gcgctgtggg cgaaatgtaa ctacgaagcc cttgaagaaa cgggcaagct cggcgagatc
    17821 cttttcttta tgcgcgccgg ttctaccggt agccagaaat actccaccat gatgtgggcg
    17881 ggcgaccaga acgtcgactg gagtctcgac gatggcctgg cgtcggttgt cccggcggcg
    17941 ctgtcgctgg caatgaccgg acatggcctg caccacagcg acattggcgg ttacaccacc
    18001 ctgtttgaga tgaagcgcag caaagagctg ctgctgcgct ggtgcgattt cagcgccttc
    18061 acgccgatga tgcgcaccca cgaaggtaac cgtcctggcg acaactggca gtttgacggc
    18121 gacgcagaaa ctatcgccca ttttgcccgt atgacctccg tcttcaccac cctgaaacct
    18181 tacctgaaag aggctgtcgc gctgaatgcg aagtccggcc tgccggttat gcgcccgctg
    18241 ttcctgcatt acgaagacga tgcgcacact tacaccctga aatatcagta cctgttaggt
    18301 cgcgacattc tggtcgctcc ggtgcatgaa gaaggccgta gcgactggac gctctatctg
    18361 ccggaggata actgggtcca cgcctggacg ggtgaagcgt tccggggcgg ggaagttacc
    18421 gttaatgcgc ccatcggcaa gccgccggtc ttttatcgcg ccgatagcga atgggcggca
    18481 ctgttcgcgt cgttaaaaag catctaatca catccgcccg tggggaaact catgggcaaa
    18541 gggagaacat cgatgagtca catcacaacg gaagatccag caactttacg cctgcccttt
    18601 aaagagaaac tctcttacgg tattggcgac ctggcctcta acatcctgct ggatatcggt
    18661 acactttatc ttttgaagtt ttataccgac gttctggggc tgccaggcac ctatggcggc
    18721 attatctttt tgatttcaaa attctttact gcgtttaccg atatgggtac cggcattatg
    18781 ttggattccc gacgcaagat cggtccaaaa ggtaagttcc gtccttttat tctgtatgcg
    18841 tcattcccgg tcaccttact ggcgatcgcc aactttgtcg gcacaccgtt tgatgtcacc
    18901 ggtaaaacgg tgatggccac tattctgttt atgctttacg gactgttttt cagcatgatg
    18961 aactgctcct acggcgcaat ggttcccgct atcaccaaaa accccaacga acgcgcctca
    19021 ctggcggcat ggcgtcaggg cggcgctacg ctgggcctgc tgctgtgcac ggtgggattc
    19081 gtgccagtga tgaatcttat cgagggtaat cagcaacttg gctatatctt cgccgccacg
    19141 ctgttttcac tgttcggcct gctgtttatg tggatctgct actcaggcgt gaaagagcgt
    19201 tatgtcgaaa cccaaccaac caatccggcg caaaagcctg gcctgttgca atctttccgc
    19261 gcaattgccg gtaaccgccc actgttcatt ctgtgtattg ccaacctctg caccttaggg
    19321 gcgtttaacg tcaagctcgc cattcaggtc tattacaccc agcacgtact taacgatccc
    19381 atcctgttgt cgtatatggg atttttcagc atgggctgta ttttcatcgg cgtgttcctg
    19441 atgcctggcg cagtcagacg ttttggtaag aagaaggtct atatcggcgg cctgctgatt
    19501 tgggtgctgg gcgatctgct caactatttc ttcggcggcg gctcggtcag ctttgtggcg
    19561 ttctcctgcc tggcgttctt cggctcagcg tttgttaaca gcctgaactg ggcgctggtt
    19621 tccgacaccg tcgagtacgg cgagtggcgc accggcgtgc gttcggaagg cacggtctac
    19681 accggcttca ccttctttcg caaagtgtct caggcgctgg ctggtttctt ccccggctgg
    19741 atgctgacgc aaattggcta tgtgccgaac gtcgcacagg ctgaccacac cattgaaggg
    19801 ttacgccagt tgatcttcat ctacccaagc gcactggcgg tagtcaccat tgtggcgatg
    19861 ggttgcttct acagcctgaa cgagaagatg tatgtccgca ttgtggaaga gatagaagca
    19921 cgtaaacgca cggcgtaata ataataaacg acgccctgcg gggcgttata aggagtgatt
    19981 atgtctgacc ataatccact gacattaaaa ctgaatctgc gggaaaaaat cgcctatggt
    20041 atgggcgacg tcggttcgaa tttaatgctc tgcatcggta ctctgtatct cctcaaattt
    20101 tataccgatg aattagggat gcctgcttac tatggcggga ttatctttct ggtggcgaag
    20161 ttttttaccg cgtttaccga tatgctcacc ggatttttac tcgactcgcg gaaaaatatt
    20221 gggcctaaag gcaaatttcg tccctttatt ttatatgcag cagttccggc ggcgttaatt
    20281 gcaacgctcc agtttatcgc caccaccttt tgtttgccgg ttaaaacgac gattgccacc
    20341 gcgctgttta tgatgtttgg actttcatac agtctgatga actgctcgta tggtgcgatg
    20401 atcccggcaa ttaccaaaaa cccgaatgag cgcgcgcagc tcgcggctta ccgccagggt
    20461 ggtgcaacca tagggctatt gatttgtacc gtggcgttta ttccgttgca gtcgcttttt
    20521 tctgactcaa ccgtcggtta tgcctgtgcg gcacttatgt tctccattgg cggctttatt
    20581 tttatgatgc tgtgctacag aggcgtcaaa gagcattatg tggacacagc gccaaccgga
    20641 cataaagcca gtattctcaa atctttttgc gcgatatttc gtaatccacc attgctggtt
    20701 ttatgcattg ctaacctgtg tactctggcg gcatttaata tcaaactggc gattcaggtc
    20761 tattacaccc agtatgtgct gaatgatatt aatttattgt cgtggatggg attcttcagt
    20821 atgggatgca tcctcgtcgg cgtattactg gtgccagtaa ctgtaaaatg ttttggtaaa
    20881 aaacaggttt atttagctgg catggtgctg tgggcggtgg gtgatatact gaattatttc
    20941 tggggaagta actctttcac tttcgtcatg ttctcttgtg tcgccttttt tggcacggcc
    21001 tttgtaaaca gcctgaactg ggcactggta ccagataccg tagattacgg tgaatggaaa
    21061 accggtattc gtgccgaagg ttctgtttat accggttata ccttctttcg taaaatttct
    21121 gccgcacttg ctggcttctt gccaggcatt atgctgacgc aaattggtta tgttcccaac
    21181 atcgcgcaaa gcgatgcgac attacagggt ttgcgtcagc tcatttttat ctggccttgt
    21241 gcactggcaa ttattgctgc attgacgatg ggattctttt acacactcaa tgaaaaacgg
    21301 tttgcattga ttattgagga aatcaaccaa cgcaaaaata aagagatggc gacagaagaa
    21361 aaaacggctt ccgtaacgtt ataaatgtca atagccgcta tttccatcct ggtgggtggc
    21421 ggcctcccta cgtttaaaaa atggacttat tatgaaaaag attaatgcaa taattttatt
    21481 atcttctctg acctctgcct cggtatttgc tggcgcatac gtcgaaaaca gggaggcata
    21541 taatcttgcc tccgatcagg gcgaggttat gctacgtgtg ggttataact tcgatatggg
    21601 cgcgggtatt atgttaacca atacctacac ctttcagcga gaagatgaac taaaacatgg
    21661 ctataatgaa attgaaggtt ggtatccttt attcaaacca accgataaat taaccatcca
    21721 gcccggtggc ttaattaatg ataagagtat cggttcgggt ggtgcagtgt atctggacgt
    21781 caactataaa tttgtaccat ggtttaatct gacagtacga aatcgctata accataataa
    21841 ctatagttca acagatttga gcggggaact ggataataat gacacctatg aaattggcac
    21901 ctactggaat tttaaaatca ccgataaatt ttcctataca tttgagccac attatttcat
    21961 acgcgtaaat gactttaata gcagcaatgg aaaagatcat cactgggaaa tcactaatac
    22021 cttccgttat cgcattaatg aacactggct gccttatttc gaattacgtt ggttagatcg
    22081 taatgtcgaa ccgtaccacc gcgagcaaaa ccagattcgt atcgggacga agtatttctt
    22141 ctgatgtgaa actgccggat gcggcgtaaa cgccttatcc ggcctacggt tgatacggtt
    22201 ttcgttggcc ggattaagca catccggcca ttaatcaagc ttcagccgcc agcatctctt
    22261 tcttacgttg gctaattcgc ttaaccagca ttacggaaac caccgcacca cagaatgcga
    22321 agcaggccat aatgccaaac acgattttgt agccaataat ccccggattt aaatccagaa
    22381 tgtagccata cagactgaag cagaacatcg ccggagcgta accaataaag ctacccaacg
    22441 ccatcgccgc gcctgtttta ttttcagcaa tttttgcttc gccgataggt gcaaaaaata
    22501 cggctcgctg tgtaaagact atcgcgccaa agcccagcgt acatgccatc cctaaataga
    22561 ccggcatact ttcgtgcggc agcataatca acagtacgag cgcagcggta ctgatgataa
    22621 aggtgtagca tagatattta ctcggcgatt tcaggatctt atctgaaatc atgccaccaa
    22681 tcggtccacc aatcattttc agacagtatt ggttaatgat gccgtaagcc cccaccagcg
    22741 caacgggcaa tagatagatg tttttcagga atggaatgaa gaatgtcagg ccacagtaaa
    22801 ccgcgtaaac aaagaagacg ttaaaagcga taagccagat ggttttgtct ctcagcaccg
    22861 aggtcatact ggtgttttgc gatgctccgt cttctttttt cacctcaacg gacggtgcct
    22921 cttctttgtc attcaggaca aagaaaataa taatgcctac ggcaatcaca ataagggaat
    22981 agaaccagat gcctgcttta aaacctaata agccactgcc aaaccaggta aatactgcca
    23041 acgcagaaaa tgccaccacg gtatcgacaa tgccacgccc tgtttcgaag aagccaaaca
    23101 accgaccttg ttgttcgcta ttgcccaatc gacttaccga cttgagcaag accggccagt
    23161 tcatcatgtc gcaagtaacg ccaaatagcg cccagacaaa gaggatgccc caataccccg
    23221 gcattgtcgt caaataaaca cccagtaatc ctgtcgcaat gagtgagaat gacatggtgt
    23281 atctgcgcgg tagtttatcg gcaaaataaa tagacagaaa aaagcccact gtggtgacaa
    23341 atgagtttac cgacatagca ttaccaattt gaccattggt caaatggaaa tattcctgca
    23401 tcgggatata aaacgcatct ttcagcgacg gtaatttata aattgtaccg ccacacagtg
    23461 ttaatagact aaataacgcc catttctttt tcgtgagcat aacatcccct aagcgatttt
    23521 taaaatcact gttttaaagg caataagata ccgaccattc atatagaatg gttttattaa
    23581 ctgcgtatga gttaaaactt attagtggtt cagaagatgg tttgcctctt gtttgatttg
    23641 ttgtagtacg ttacgcacat gatttatctg attaattgca tcttcttttt ctttacgtaa
    23701 ccgcgcatcc agtaaatgat tttctggtag gggtgtttca ctcatctggc gataagggat
    23761 ccacggatta tcatcttcgt cttcaaagcg gaacgccggc gcatagtaat caacctcttc
    23821 ttccaggcca tatgccgtca cctgcggctc atcatcacca aggcagataa ggtgcgttaa
    23881 taacgctttg aacggcatat caccctgacc tgaaatacag gctttatgac ccaggccacc
    23941 ctgttcttta acgatcaagg catctttaat atggacctgg gtaatatgcg gtgccatcgt
    24001 ttttaaggcg tcgatgggat gctcatttgc attgatcatg ttcgcaaaat caaacagtaa
    24061 ggaaagagac tccatctcac tttctttgac cagcgacacc aactcatgac tttttaaatc
    24121 ttcatgctgc tcgatcgtaa aagtcaggcc gctgtcctga tacgtttccc gtacataggc
    24181 aatgtcgtta gcgataatcg ataatacgtc gcgcaaatta ccttcataac gtggataaaa
    24241 acgtacggac gatgccccag ttttcaacgc gatggcgacg gcttcgtcaa tagatgcctt
    24301 atctgaggcg ctggtttcaa tatgaatatc aaggttcaga cggcgggctt tatccccaaa
    24361 ggcagagagt tctttatcgt ccatattacc aagagaaaaa cgctcgccat ccagaacatg
    24421 gattttgacg ccacgtagat tattttcact tgcgatatca agtaaatcag caggcaacac
    24481 tctttccagc cgcatattta aatgaaaggc ataagcatgt aaataaagag gaagagtgtc
    24541 gacacgttgt agaatctttc ttgcgttatt tattgtcacc ataaaacacc tacagattaa
    24601 tgatgaggtc tacggcctca aatcgccaat attcaatatc tatctggaca atattgcctt
    24661 gctggtcggc gcgatgcttt tcaattaaga tcgctggcat acccgcagaa ccgccaagat
    24721 acttgctgat atcgcccggc attctgacgg gtttgaaaat caattttttt actaccgtct
    24781 ctttttggta gcacttttcc cagactgcag aaaatgagtg gttttcaagt tgttcaataa
    24841 aacccggagc aacttcagga ttaatatatg tttcgtgata gaaaacttta tgtccttcca
    24901 gcgcccccca gccggtgatg cgatagagtt cagttgatgg tgtcactgca atcagtggcg
    24961 cgagcgtctt gggaatatcg ctggtacggt ttttctcggt aaacccccag gaaggctctc
    25021 gtccttgctc aattgcggcc cgctgaaagc tcgccgacaa ctccggactg taattaaatc
    25081 gcggctgggt cacgaaccac cccttacgat ccttacggaa gattttggat tctgcctcga
    25141 ggttcaacaa cgcctggcgc agcgtcatac gtttaatgcc cagcagttct cccagctctc
    25201 tttctgaagg gagttttcca cccggcgtgg tgatgccatc tttcaaccac cgatccagtt
    25261 tctcttttgc attttctacg gtggattgat tctccgccat tgtttaatcc ttttggttta
    25321 aaccagatga agcatcttac gcttacgcca aaaaagaaag caattaacgg cgtatgactt
    25381 tgcagataat tgtgacaaag ctctctattg acgtaatccc agacttataa gggagatact
    25441 cgcattggtg tgcgctcacg acaggtaaaa aaaaacctgc cagcgatggc aggtttttta
    25501 taactaaaaa acgaaattaa tcgtctttcg gtgcgcggtt ggcgcgacga cgatcgtttt
    25561 cagtcaggtg acgtttacga atacggatag aggtcggagt cacttctacc agttcgtcat
    25621 catcgatgaa ctccagagct tgttccagag tcatgcggat aggcggaacc agaacaacgg
    25681 cttcgtcagt accggaagca cgcatgttgg tcagtttctt accagtcagg cagtttaccg
    25741 tcaggtcgtt agagcggctg tgaataccga taatctgacc ttcgtaaact tctgcaccgt
    25801 gaccgaggaa cagcttaccg cgatcctgca gaccgaacag cgcgaacgcg accgctttac
    25861 cctgaccgtt agagatcagt acgccgttct gacgctgacc cacttcaccc ggacgtacgt
    25921 cgtcgtagtg gctgaaggtg gagtacagca gaccagtacc ggaagtcatg gtcatgaact
    25981 cagaacggaa gccaatcaga ccacggcttg ggatcacgta gtcgagacgt acgcggcctt
    26041 taccgtctgg attcatgttt ttcaggtcgc ctttacgttc gcccagcgcc tgcattacag
    26101 aaccctgatg ctgttcttca acgtccagag tcacgttttc atacggctct tgtttacgac
    26161 cgtcgatttc acggaagata actttcggac gggataccgc cagttcgaaa ccttcacgac
    26221 gcatgttttc gatcagaaca gacaggtgca gttcgccacg accagaaacg cggaacgcat
    26281 cggcgtcttc ggtttcttct acgcgcagcg caacgttgtg taccagttct ttgttcagac
    26341 gatccaggat ctgacgagac gttacgaact taccttcttt accgcagaac ggcgaggtgt
    26401 taacgcagaa gaacatagaa acggtcggct catcaacgga gagtgccggc agcgcttcaa
    26461 cgttttgcgt gtcgcaaacg gtgtcagaaa tgttcagttc gccaaggccc gtgatcgcaa
    26521 cgatatcgcc agcttccgcc agatcggttt cgatacgttc cagaccgagg tggcccagca
    26581 ctttaccgac tttcgcgttg cgggttttgc cttcgctatc gatgatagtg acctgctggt
    26641 tcggcttcac tttaccgcgc ttgatgcggc caatgccgat aacgccaaca tagctgttgt
    26701 aatcgagctg agaaatctgc atctggaacg gaccgtcaag gtcaacgtcc ggcgcaggaa
    26761 cgtgatcaac aatcgcctga tacagcgggg tcatgtcttc cgccatatct tcgtggtcca
    26821 gacccgcgat accgttcagc gcagaagcgt aaacgatcgg gaagtccagc tgctcgtcgg
    26881 tcgcgtcgag gttaacgaac agatcgaata cctgatccac aacccaatca ggacgcgcgc
    26941 cagggcggtc aactttgttg ataacaacaa tcggcttcag gccgtaagca aacgcttttt
    27001 tggttacgaa gcgcgtttgc ggcatcgggc cgtcaaatgc gtcaaccacc agcagcactg
    27061 agtctaccat ggacattaca cgttcaactt caccaccgaa gtcggcgtgc cccggggtat
    27121 caacgatgtt gatacggtaa tcattccatt tgatagcggt gtttttcgcg aggatggtaa
    27181 tcccacgctc tttctccaaa tcgttggagt ccatcacgcg ctcttgggtt tcggcacgag
    27241 agtcgaacgt accggattgt tggagcagct tgtctaccag ggtggtttta ccatggtcta
    27301 cgtgcgcgat gatggcgata ttacgcaatt tttcgatcac aactttgcct caggcattag
    27361 aaatagcgcg ttattgtaca cggattaatc gcactacaaa acaggatcac aaacatcctc
    27421 cgcaaacaag tattgcagag tccctttgtg atcgctttca cggagcataa aaagggttat
    27481 ccaaaggtca ttgcaccaac atggtgctta atgtttccat tgaagcacta tattggtgca
    27541 acattcacat cgtggtgcag cccttttgca cgatggtgcg catgataacg ccttttaggg
    27601 gcaatttaaa agttggcaca gatttcgctt tatctttttt acggcgacac ggccaaaata
    27661 attgcagatt tcgttaccac gacgaccatg accaatccag gagagttaaa gtatgtccgc
    27721 tgaacacgta ctgacgatgc tgaacgagca cgaagtgaag tttgttgatt tgcgcttcac
    27781 cgatactaaa ggtaaagaac agcacgtcac tatccctgct catcaggtga atgctgaatt
    27841 cttcgaagaa ggcaaaatgt ttgacggctc ctcgattggc ggctggaaag gcattaacga
    27901 gtccgacatg gtgctgatgc cagacgcatc caccgcagtg attgacccgt tcttcgccga
    27961 ctccaccctg attatccgtt gcgacatcct tgaacctggc accctgcaag gctatgaccg
    28021 tgacccgcgc tccattgcga agcgcgccga agattacctg cgttccactg gcattgccga
    28081 caccgtactg ttcgggccag aacctgaatt cttcctgttc gatgacatcc gtttcggatc
    28141 atctatctcc ggttctcacg ttgctatcga cgatatcgaa ggcgcatgga actcctccac
    28201 ccaatatgaa ggtggtaaca aaggtcaccg tccggcagtg aaaggcggtt acttcccggt
    28261 tccgccggta gactctgctc aggatattcg ttctgaaatg tgtctggtga tggaacagat
    28321 gggcctggtg gttgaagccc atcaccacga agtagcgact gctggtcaga acgaagtggc
    28381 tacccgcttc aataccatga ccaaaaaagc tgacgaaatt cagatctaca aatatgttgt
    28441 gcacaacgta gcgcaccgct tcggtaaaac cgcgaccttt atgccaaaac cgatgttcgg
    28501 tgataacggc tccggtatgc actgccacat gtctctgtct aaaaacggcg ttaacctgtt
    28561 cgcaggcgac aaatacgcag gtctgtctga gcaggcgctg tactacattg gcggcgtaat
    28621 caaacacgct aaagcgatta acgccctggc aaacccgacc accaactctt ataagcgtct
    28681 ggtcccgggc tatgaagcac cggtaatgct ggcttactct gcgcgtaacc gttctgcgtc
    28741 tatccgtatt ccggtggttt cttctccgaa agcacgtcgt atcgaagtac gtttcccgga
    28801 tccggcagct aacccgtacc tgtgctttgc tgccctgctg atggccggtc ttgatggtat
    28861 caagaacaag atccatccgg gcgaagccat ggacaaaaac ctgtatgacc tgccgccaga
    28921 agaagcgaaa gagatcccac aggttgcagg ctctctggaa gaagcactga acgaactgga
    28981 tctggaccgc gagttcctga aagccggtgg cgtgttcact gacgaagcaa ttgatgcgta
    29041 catcgctctg cgtcgcgaag aagatgaccg cgtgcgtatg actccgcatc cggtagagtt
    29101 tgagctgtac tacagcgtct aatagttgaa gttgtactac ccggcgcaac aacgccggag
    29161 gttcaaacca ggcccatctg gcgtaattgt tgcagccagt ttgaacacgg acagcgcgca
    29221 gaacccggag cgtacactgg tacgtgagga gttcgagcac tgcccaggtt caaaatggca
    29281 aataaaatag cctgatggga ctggttttta gttgccgtgg aaatttcagc ccatctctgc
    29341 atgggctttt ttctccgtca attctctgat gcttcgcgct ttttatccgt aaaaagctat
    29401 aatgcactaa aatggtgcaa cctgttcagg agactgcttt atggcaacag gcacgcagcc
    29461 cgatgctggg cagatcctca actcgctgat taacagtatt ttgttaatcg atgacaacct
    29521 ggcgatccat tacgccaacc ctgccgcgca acaactgctc gcccaaagct cccgcaaatt
    29581 gtttggtaca ccgttaccgg aactgttgag ctacttctca ttaaatatcg agctgatgca
    29641 agaaagtctg gaggcggggc aaggttttac cgataacgaa gtgacgctgg tcatcgacgg
    29701 gcgctcgcat atcctttctg tgacggccca gcgtatgccg gacggcatga tcctgctgga
    29761 gatggctccg atggataacc agcgccgctt aagtcaggaa cagctacagc acgcccagca
    29821 ggttgctgcc cgtgatttag tgcgcggcct ggcacatgag attaaaaatc cgcttggcgg
    29881 tttacgtggc gcggcgcagc tactcagcaa agcgttacct gacccatcac tactcgaata
    29941 taccaaagtg attatcgaac aggcggaccg gctgcgaaat ctggtcgacc gtctgttggg
    30001 gccgcagctg cccggtacgc gcgttaccga aagtattcac aaagtagctg aacgcgtggt
    30061 aacgctggtg tcgatggaac tgccggacaa cgtgcggttg attcgtgatt acgatcccag
    30121 cctgccggaa ctggcgcacg acccggatca aattgaacag gtcttgctga atattgtgcg
    30181 caatgcgcta caggcgctgg ggccggaggg tggtgaaatc attctgcgta cccgcaccgc
    30241 gtttcaactg accttacacg gcgagcgcta ccggctggcg gcgcggattg atgtggaaga
    30301 taacgggccg ggcattccgc ctcatttgca ggatacgctg ttttacccga tggtcagcgg
    30361 ccgcgaaggt ggcaccgggc ttggcttatc catcgcccgt aatttgattg atcagcattc
    30421 aggcaaaatt gaatttacca gttggccagg tcataccgag ttctcggttt acctgcctat
    30481 caggaaataa aggtgacgtt tatgcaacga gggatagtct gggtagtcga tgacgatagt
    30541 tccatccgtt gggtgcttga acgtgcgctc gctggagcgg gtttaacctg tacgacattt
    30601 gagaacggcg cggaagtact ggaggcgctg gcgagcaaaa cgccggatgt gctgctttca
    30661 gatatccgta tgccgggaat ggacgggctg gcgctgctca agcagattaa acagcgccat
    30721 ccaatgcttc cggtcatcat tatgaccgca cattccgatc tggatgctgc cgtcagcgcc
    30781 tatcaacaag gggcgtttga ttatctgccc aaaccgtttg atatcgacga agccgtcgcg
    30841 ctggttgagc gcgccatcag tcattaccag gaacagcagc agccgcgtaa tattcagctt
    30901 aacggcccaa cgaccgatat catcggcgaa gcgccagcca tgcaggacgt gttccgtatt
    30961 atcggtcggc tttcgcgttc ttctattagc gtgctgatta acggcgaatc cggcaccggt
    31021 aaagaactgg tcgctcatgc cctgcatcgc cacagtccgc gcgccaaagc gccgtttatc
    31081 gcgctgaata tggcagctat cccaaaagat ttgatcgaat cagaactgtt tggccacgag
    31141 aaaggcgcgt ttactggcgc gaataccatt cgtcaggggc gttttgaaca ggccgatggc
    31201 ggtacattat tcctcgacga aattggtgat atgccgctgg atgtgcagac gcgtttgctg
    31261 cgcgtgctgg cagacggtca gttttaccgc gttggcggct atgcgccggt gaaagtggat
    31321 gtgcggatta tcgctgccac tcaccagaat ctcgaacagc gagtgcagga aggtaagttc
    31381 cgtgaggatc tgttccaccg cctgaacgtt atccgcgttc atctgccgcc gctgcgcgaa
    31441 cgtcgggaag atattccccg tctggcgcgc cattttttac aggttgccgc gcgcgaactg
    31501 ggcgtagaag cgaagttact gcatccggaa accgaagctg ctctgacgcg tctggcgtgg
    31561 ccaggcaacg tgcgccagct ggaaaacacc tgccgctggc taacggtgat ggccgccggg
    31621 caggaagtgt tgattcagga tttgcccggc gaactgtttg aatcaacggt tgcggagagt
    31681 acttcgcaaa tgcaaccgga cagctgggcg acgcttcttg cgcagtgggc agacagagcg
    31741 ctgcgttccg gtcatcaaaa tctgctttcc gaagcgcagc cagagctgga gcggacgtta
    31801 ctgacgaccg cgttgcgaca tacgcagggg cataaacagg aagcggcgcg gctactcggc
    31861 tggggccgca acaccctgac gcgtaagtta aaagagctgg ggatggagtg attcacggcg
    31921 tgtgtgtaaa gattgattat tgagcgcaaa ttgctggtat tttacgcttt actgttccga
    31981 taagttcagt atgatcttgc ccggaaaacg gggagagtca ttatgctgga atcaataatt
    32041 aatctggtat cgagtggcgc agttgacagc cacacaccgc aaactgccgt tgctgccgtg
    32101 ctgtgtgccg caatgattgg gctgtttagt tgagtttcag attgctgatg aagtgctctt
    32161 tgttcatgcc ggatgcggcg tgaacgcctt atccggccta caaaagcaat attttcaaca
    32221 ggttttgtag gcctgataag cgtagcgcat caggcaatct tgttctcagc acattgatgg
    32281 cgctttcgtt ttctactttg taaacgaagc gccattcact atttaaatca cccgtgagaa
    32341 ctgctgcatc cgcgctttct ggcgcagata ggtatcaaag cacatgcaaa tgttgcggat
    32401 cagcaagcga cctttcgccg tcacctgtat tcccttctca tccacatcca ccagcccatc
    32461 ttttgctaac ggggcgagca gcttgagatc ttccgcaaag taatcagcga agtgcaaatc
    32521 ccactgtttc tcaatagggg cgtaatccag acggaagttg cagatgagcg acttaatcac
    32581 atcgcggcga atacagtcat cacgcgttag cgcaatacca cgccacagcg cattgccttg
    32641 ttcatccact tgctgatagt actgcttcaa ctctttctgg ttctgcgcgt agcagtcgcc
    32701 aatcatgctg atggcggaaa cgcccatccc cagcagatcg gtatcgccct gagtggtgta
    32761 gccctggaag ttacgatgca gcacgccttc acgctgggcc accgccagct cgtcatccgg
    32821 acgggcaaag tgatccatac cgataaactg atagcccgat tgcgtcagga aggcgatggt
    32881 ttcctgcagg atatcgagtt tttgctgcgg actcggcagg tcagcatctt tgattttgcg
    32941 ctgagcagca aaaatggtcg gcagatgcgc gtagttaaag acactcagac gatcggggtt
    33001 cagctccgcc acacgtttca gggtaaaggc gaaactctcc ggcgtctgtt tcggcaggcc
    33061 gtaaatcagg tcgatgttgg tggaggtaaa accaatctca cgcgcatggt taagcagtgc
    33121 aaagatgaac tcttcatcct gctcgcggtt aaccagacgt tgcacttctt tgttgaagtc
    33181 ctgcacgccc atgctcaggc gattaaagcc ttcggcgcgt aaatgatcga gtacatccag
    33241 ttcgatttcc cgcggatcga cttcgatcga aatctccgca tcggcattga actggaagtt
    33301 ttcgcgcagc agcttcatca ggcggctgat ttgcgcttta ttcagatacg tcggcgttcc
    33361 gccgccccag tgcaattggc tgacgtgacg cccggcgaac agcggtgcac gatggacgat
    33421 ttcttgctcc agcgcgtcca gatactgatc ggccttgtgc tgctggcgag taacaatctt
    33481 attgcaaccg cagaagtaac aaagcttatg gcagaacggg atatgtacgt agagagataa
    33541 tggacgctca ggatagcgcg ccacggcttg taaaaacgcc tgttcgccga agtcttctga
    33601 aaactccagc gcggtcgggt acgaggtgta tcgtggcccg gaatagttat atttctggat
    33661 cagggccaga tcccagtcga tttgctgtac agacatgctc actccttccg atggcgtctc
    33721 tggcggctac ggcgtaccgg cggatgcccg tttcgggtca tcagccggtt gcgcagccac
    33781 ttttgtcgcc gcgacaaccg ctgtagttta acgaataacc acaccagata acatataacc
    33841 ggaagggtta tcagcagaac gggaaggccc acggcggcaa tccgttagtt gccccgcagc
    33901 agacgcatca tgtcttcttg cttctcgtct tcttcctctt cttcgtcatc gtcataagag
    33961 aggccgagtt tctgcatcaa ctcatcaata cgatccagtt tagcatccac ccaggattgc
    34021 tcttcggcgc tcagggtttc gcctgcttcc agacgttcca gcagcgcatc cagacgctca
    34081 tccgtttcca gtaattccaa ctccgcctgc ggtgaaagca taggtttctc actcttcggt
    34141 ttgtgctgtt tggtgacttt ttcaatcacg cccaatggaa taggggtttt actgccaata
    34201 cgtggatctt ttggtgcgtt ctggcctttg ctgcctgacg tggtgtttcc gcccgctgcg
    34261 cggctgcccg gcgcatgacc acgacgtttt ttctgacgct tgcgatcacg agcttcctga
    34321 tccagctcct cgcgtgtttt tcgacgcgct tttgcatgac ctttgctgcg tgagtttgaa
    34381 gatgatggtt tcataatggt tcgtctttta gccgttttat tcagtataga tttgcggcgg
    34441 aatctaacag aaagcaagca aagaaaaaag gcgacagatt actctgtcgc cttttttcct
    34501 gactcataac ccttaacggg tcttacaatc cttgcaggcc gccaacacac cctgtttttc
    34561 ctttagccgg aacaatccgt gttgattcca tttccgttta attacgtctc cggacgtttg
    34621 tcttcctgac aatcctctgt cttcgcctcc tggcgctcct gaccctcatc ctgagtcatt
    34681 gttcctgtta gcgtcctcgc tctatgggtg ctactttacg cctttgcttt aaacaaacaa
    34741 tcaaccgcca tcagtatttg cacattttca gagttttata taaaagcaat tcactgattt
    34801 atatagctta tttgacatgc atcgcagaat tttctctgct atttcgcata aagactatcg
    34861 gcaatgtctc acaaagcgta tggcttttac ttacaacgct cacgccgaaa aatccgcctt
    34921 ttattatggt tcaggtataa tcgccacaaa gctttgactg acggagacga ccgctttgac
    34981 taatttgaat tatcaacaga cgcattttgt gatgagtgcg cctgatattc gccacctacc
    35041 ttccgatacc ggaattgaag tggcttttgc aggccgttcc aacgcaggta aatccagcgc
    35101 gctgaacacg ctgactaacc agaaaagcct ggctcgtacc tcaaaaaccc cagggcgcac
    35161 ccagcttatc aacctgtttg aagtggctga cggcaagcgt ctggttgact tgcctgggta
    35221 cggttatgcg gaagtcccgg aagagatgaa gcgcaaatgg cagcgtgcgc tcggcgaata
    35281 cctcgaaaaa cgtcagagcc tgcaaggtct ggtggtgcta atggatattc gccatccgct
    35341 gaaagatttg gatcagcaga tgattgagtg ggcggtagac agcaatatcg ccgttctggt
    35401 gctgctgacc aaagcggaca aactggcaag cggcgcacgt aaagcgcaat tgaatatggt
    35461 gcgtgaagct gtactggcgt ttaacggtga tgtgcaggtt gaaacgtttt cttcgttgaa
    35521 gaaacaaggc gtggacaagc tgcggcagaa actggatacc tggtttagcg agatgcagcc
    35581 tgtagaagaa acgcaggacg gcgaataatt ttcttgcctt aatgcttgtg ccggatgtgg
    35641 cgtatccggc ccgtaaattc aatagattac aattctaccg ttggcctgat aaacatagcg
    35701 catcaggcat tacggatctt ttctttcgcc caataaaaaa cgccccagtc attactgact
    35761 ggggcggcta aaatattcag ccaaatccga ttacgtgaag taaaaggtct gaaagataga
    35821 acatcttacc tctgtaccct acgcgactaa ctttactctt ttttgttcag ttcagaaagc
    35881 actttttgta attttttttc agttgttgca taggaatttc taatagccat cacaaaacgt
    35941 gacagcttat gttgcttact tacgaaaaaa gggcatgttc aggcgaatct tagtgcgcct
    36001 gatcccagtt ttcgccactc cccacttcca ccagcaacgg cacatccaga cgggtacagt
    36061 tttccatcag ttgatgaatc tgcttcgcga cggcatcaac atcatcttta tgaacttcaa
    36121 ataccagttc atcgtgtacc tgcatgatca tacgtacacg cggttgctca gcctgtaacc
    36181 acgcatcaac ggcaatcatc gcccgtttga taatgtcggc ggcggttccc tgcattggcg
    36241 cgttaatggc tgcacgttca gccgctgcac gacgagcacc attgctggat ttgatatccg
    36301 gcagatacag acggcgtccg tccagcgttt caacgtagcc ctgctctttc gcctgagcac
    36361 gggtgcgttc catatactcc agcacgccag ggtagcgttc gaagtaaagg tccatgtact
    36421 tctgcgcttc tttacgtgga atgttcaatt gccgcgccag accgaaagca ctcatgccat
    36481 aaatcagacc aaagttgatc gctttcgcgc tacggcgttg ctcgctggtg acggtttcca
    36541 gtggcaaacc aaacacttct gccgccgttg cccggtggat atcttttcct tccgcgaatg
    36601 cggtcagcaa gcctttgtca cgcgagagat gcgccataat gcgcagttca atctgcgagt
    36661 agtccgctga gacaatcaca taatcctctg gcgcaataaa cgcctggcgg atacgacgac
    36721 cttcttcgtt acgcaccgga atgttttgca ggttaggatc ggttgacgat aaacgtcccg
    36781 ttgcggttac tgcctggtga taagaggtat gcacacgccc ggttttcggg ttgatcatca
    36841 gcggcagctt gtcggtgtaa gtcgacttca gcttcgccag accacgatac tccagaatca
    36901 cttttggcaa cggatagtcc agcgccagtt cttccagtac ctcttccgac gttgacggcg
    36961 cgccacccgg cgttttcttc agcggtttaa taccctgttt ttcaaacaga atggtttgca
    37021 actgcttggt ggaagaaagg ttaaattctt cacctgcaat ttcatgcgct ttcttttcca
    37081 gctcagccag acgcaaggtg agctcttcag aatgattgtg cagcactttc ggatcgatct
    37141 tcacaccgtt acgttcaatg cgtgaaagca ctggcaccag cggcatttcg atattctcga
    37201 agacgttcaa cggccctttg tgtttttgca gatccggcca cattttcaga tgcaactgca
    37261 aggtgacatc agcatcttcg gcggcgtaac gtccggcctc ttcgagggca atctggttaa
    37321 aggtcagttg atttttgcct ttacccgcaa tctcttcaaa agtgatggtt ttgtgcttca
    37381 accaacgttc cgcgaggctg tccatatcgt gacgcccggc aacgctattg agaatgtagg
    37441 actccagcat ggtatcaaac gcaatcccac gcagctcaat gccgtaattc gccagaatac
    37501 cgcgatcgta tttcaggttt tgcccgacct tcagcgcctt ttcatcttcc agcagcggtt
    37561 ttagcaactc gagtgcacgc tcgcgagaga tttgatcggg cgcgtcaaga taatcatgag
    37621 caaccggaat atatgccgct acgcctggct cgatagcaaa agaaagcccg accaggttag
    37681 cagagatgtt atcaaggctg tcggtttcgg tatcaaatgc aaataccggc gctttttcca
    37741 gcttcgcaat ccacgctttc agtgtttctt catcaaggat ggtgacgtag ttgtcataag
    37801 aaatcaccgt tgccgtcact tctggcgctt cgtctgcaac actggtttcc tgcggcttcg
    37861 cggctggttt tgcccctttg gcctgtaacc atttgcccgc ttcgacatca gcagtccagc
    37921 gtttgaactc atactttttg aacagcccca acaactcttc cgctgccggt tgctgcactt
    37981 ccagttgttc acaggtcagc tccagttcaa cgtcggtttt aatcgtcgcc agctggtatg
    38041 agagataagc aacttctttg ttttgctcga gcttcgctgc cattgttttc gcgccacgga
    38101 agctcaaccc agcaattttt tctggctcgg catacagcgt atccagcccg ccaagacctt
    38161 gcagcaatgc ctgcgcggtt ttttcaccga cgcccggtac gccaggaatg ttatcggagg
    38221 agtcacccat cagcgccagg aaatcgatga tcagttctgg cggcacgccg tacttattca
    38281 ccacctcttc cggtccgagg atggtattcg tcatggtatt gataagcgta atatttggcg
    38341 tcaccagctg cgccatatct ttatcgccag tgctgatcag caccggacgc ccggcttttt
    38401 cggcttcgcg cgccagagta ccgataacgt cgtccgcttc tacgccagaa accgccagca
    38461 gcggcagtcc catcgcttta accatcgcgt gcaggggttc gatttgtgct cgcagatcgt
    38521 tcggcattgg cgggcgatgt gatttgtaat gttcaaacag ttcatcacga aaggtttttc
    38581 ccttggcgtc aaagaccacc gctgcatgcg tcggtttata ttgcatgatc agactgcgca
    38641 gcatgttgag gacaccatac atcgcaccgg tcggctcgcc tgcgctgtta gtcagcgggg
    38701 gaaacgcgtg atatgcgcga taaagataag atgaaccatc tacaaggata agtggatttt
    38761 gggggatctg aaccataatg tccgtgcctg tttatcagat tatgggtaaa ggatgccaca
    38821 gacaggatga aaacatgagt ttttcaccgc ttttcgtgaa aatcttttgt agatcttctg
    38881 gatcgctcgc aaatcgtcat gtggataact ttgtgcataa attatttaca tcgcttttaa
    38941 tttattatca tgctttttac agcatgatgt ttgtctttgt ttttcatgca gttaaaaata
    39001 aagccatccc taataattga gataatggga ttttttctaa tgtggataga tatgaattat
    39061 ttttctcctt aaggatcatc cgttatttgg gtcgttttct gctaaaatct gcgccttgcg
    39121 cttctttagc gcactgtcta tggctaacct tttgaataaa ttcattatga cgagaatact
    39181 cgctgcgata acccttttgc tgagtatcgt attgaccatt ttggtcacta ttttttgttc
    39241 cgtcccgatc atcattgccg ggattgtaaa actattgcta cctgtgccag tcatttggcg
    39301 aaaggtttcg cgtttttgtg attttatgat gtattgctgg tgtgaaggtc tggcggtatt
    39361 actgcatctg aacccacacc tgcaatggga agttcacggg ctggaagggt taagtaagaa
    39421 gaactggtat ctgcttattt gtaaccaccg tagttgggca gatattgtcg tactgtgcgt
    39481 gctgtttcgt aagcacattc cgatgaataa atatttcctc aagcaacagc tggcctgggt
    39541 gccatttctt ggcctggcgt gctgggcgct ggatatgccg tttatgaagc gttattcccg
    39601 cgcttattta ttacgtcatc cggagcgacg tggtaaagat gttgaaacca ctcggcgttc
    39661 ttgtgaaaag tttcgcctgc atcccaccac cattgtcaat ttcgttgaag gctcccgttt
    39721 cacgcaagaa aaacatcagc aaacccactc cacttttcaa aacttgttgc caccaaaggc
    39781 ggcaggcatt gcgatggcgc taaatgtact gggtaaacaa ttcgataaac tgcttaatgt
    39841 tacgctgtgt tatccggaca ataaccgtca gccgttcttc gatatgttaa gcggtaaact
    39901 gacgcgaatt gtcgttcatg tggatttaca gcctattgcc gatgagttac atggcgacta
    39961 catcaatgac aaaagcttta agcgccattt tcagcaatgg ctgaactcac tgtggcagga
    40021 aaaagaccga cttctgacgt cgttgatgtc atcacagcgg caggacaagt aaattttgct
    40081 cgcctctgaa tgagcagagg cgagtgagta ttttaatgaa caaaacgtcc ggcacgagac
    40141 ataaattctt cttcgctcat ctcctgtccg ttaaaaacaa ctttacccgg tgtataacgc
    40201 agttgcagcg aggcggtatt gttgtcaatc gtgattaact gaaacatctg acctaaggtc
    40261 atcatcccgc tgatttgttt atcagcccgc ttttgcgctt tttccgcatc cattccttca
    40321 gataaattaa gctgttttgc ggtttctgtg gcgacattaa gcggtaactt cacatcaaaa
    40381 ttgagcgatt taatatcttt gtttgtggat gatgaagatt tggctgggtc ggcaatactg
    40441 atatccagat tggcatttag ttcgccgagt gcgttcttcc attttaccgg ttgtttaatg
    40501 gtcggctcac ttttttgtaa taacggtaaa tactctttga acaatgcagc attcacttct
    40561 tgcagagcaa cttcatcgtt tgctaactca gggtgtgcag caagctgctt ctgcattgca
    40621 atgttatact ggatgataaa ttggcgcacc gctgagggat caatagattc agcaatcaat
    40681 gaccactcac cactaccgag ttgctgattc ccttttgtta acttatcaag ttcgtaggtc
    40741 agattgacat tgatataatc tttgtcttta acgcgatcca gcatggtttt tgcaacgaaa
    40801 gcatcgattt gggcaacgtc ttccccgtga ttgataatgt taatctgatt aaatttcgac
    40861 tcgctttcac caaccggaaa tttcttttct tctaatcgcg ccagagaatc aatggtaaaa
    40921 ctttttgccg ttactttgtt cattggtgat aattgcagaa caatatcttc gactttgcct
    40981 tcaatatcca ggttagacaa tgaactacca tcaccattaa aagtaaactg gccctcacca
    41041 aaagaaaact ttccatactc tgcaggggca acatctaatg tcgtagagaa tgagccacca
    41101 aacgcgaatc gcaatgtcgc ttccacgggc gatttatttt tcgcggcgat aaatagcggt
    41161 tgtgtcagtt cattattcac taagttcact tttgccgctg ccagtgctgg gatgacatta
    41221 ccatgcatta acattgtgat gggcaacgga ccgtgctcaa catccacatc aaatacaact
    41281 ttctggcctg gcttgatatt gagatcgggt gcaccgttat cgaatgtcat ttgcatttga
    41341 aaacgagaat taaaaaaacc tttgtcaaaa tttttataac ttaacgtcat atcgtaggca
    41401 tgttcacctt tctttttagc atcgttaaaa tctttaataa acttttcgac accaggctga
    41461 atttgcgtac ctgtgtacca tgtgccacca ccccagatta cggctaacgc aacaataaca
    41521 cctgtagctg acttatgtat catataaata ttccctgata aaacagcaaa actttaaata
    41581 tccgcttatg ctgaaataat gtccagcggc aggatgcatt atcagaatgt atttatattt
    41641 attaaataat aaaaaaagcc cgtgaatatt cacgggtttt atgtaattta catttaatta
    41701 ttttttctcg gacagatatt tcactgtatc agcatactgc tgaacaaaaa catccatatt
    41761 gctggtatcc atgccctgcg gattcagctg atatttaccg ttaacaaaca tcgccggaac
    41821 accacgcaat tgcacgtcag ctgcagcttt ttcctgctga gcgaccagag atttcaccac
    41881 gaagctgttc cacgccgcgt cgtactcttc acctttaata cctgcgttga taaatacatc
    41941 gcggatatca gatgctgaac gaatggtctg ggttttctgt acgccttcaa acagcggaac
    42001 agtcactttg tcttccacgc ccagcgccat cgccacagcc catgcctgag tcagatcttt
    42061 gcccaggtca ccacccatga agttgacgtg gtatttagtc atcttcacgc cttccggcag
    42121 ttttttcttc acattatcag aaatatgcag aacttcttca aactgatagc agtgcgggca
    42181 gaagaaagag aaaaactcca gcacttgcgg cgcgccagct accggttttt ccagggtagt
    42241 gtactgttta ccatcttcat actgcgccgc cgatgcgcta aacgctaaaa ctaaaccagc
    42301 cagcgccagc caaatctttt tcatgatcta ctctctccga ttaatacata ggtgttaatt
    42361 gtaaaggggg ttcttgtaga acttttgcct gttctataaa agtcgccgtt tgtcgcagcc
    42421 agtaatcttc cccggttaac cacgggaaat ttttcgggaa cgcgggatca gcccaacgcc
    42481 gcattagcca ggcaagataa taaaccaaac gcatggcgcg taaaggttca atcagtccga
    42541 tttcagcggt gtcgaactcg ctaaattctt cataagcttc aataatagtt tccagttgca
    42601 tccgctgctc ggctttatcg ccattgagca acatccacaa atcctgaata gctggaccat
    42661 tacgtgcatc atccagatca acaaacattg gaccatcgcg ccagagaata ttcccggcgt
    42721 ggcagtctcc atgtagccgc agaacggtga agtcttcccg ccagtgtgct gtaacggcgg
    42781 cgatcagctc atctgtcgct ttcaggaatg ccgctttcaa cccggaaggc atcagtgtag
    42841 cgtcctcaaa cagcttgcgt ggctcaatga gatattcatt cagaccgatg gtcgggcgat
    42901 ggataaaaag ctgtttgcgc cccgtctggt gcatacgccc taaataacgc ccaaccgctt
    42961 ccatctgatc gatattatca gcttcgaact ggcgaccacc gacgcttgga aaaacagcga
    43021 aataaaatcc ctgatgattc aataaagtct gaccgttaaa ggccacaggt gctgcgaccg
    43081 gaacttcatc atttaccagc tgcaacgcaa attgatgttc ttcgaggatt tgatcggctg
    43141 tccaacgttc agggcgataa aatttgacga caaaacgtcg acgatcttcg tcctgaaatt
    43201 gatagacacg gttttcatag ctgttaagcg gggtaagacc ggaatccacc cggatcccat
    43261 gctcaaacag agcgtccatg atggtatccg ggtgtagtgt ctggaaagta aaagcgctgt
    43321 tattcatccc atcatccgga aaacattacg aatgacgaag gatatcattt cacgatgatt
    43381 ttggtggcgc cgcttacaag cttttactct ttaattacgc cgcgagcgcg taacagcgcc
    43441 gttttgaagt cttcttcata atctttctgc aaacccggga tcaccgcatc tttcgcggaa
    43501 tcacgcattt tcaggtgata gatcagaatg tcatccgtca aatccgccag ttcgccgtca
    43561 aaacctgact ctttcgccag tttctgcaaa aattgcagca ggttaaggtc tggctctttt
    43621 tgccaggctg gctggaggag ttcaataact tcattcagac gtttacattt catagtgatg
    43681 ctccttactc ttgagacaga cacgttagca gggtcaatcc cacaataaaa gaggcgatat
    43741 cggtgaatct gatgacgacg ataacaggcg ttgtgctggc aggcggtaaa gccagacgaa
    43801 tgggcggcgt agataaagga ttgcttgaat taaacggcaa accattatgg caacatgtcg
    43861 ctgacgcgct tatgacgcag ctctctcacg tcgtggttaa tgctaatcgt catcaggaaa
    43921 tctatcaggc aagcggtctg aaagtgattg aagattcact ggcggattac ccaggccctc
    43981 tggcaggaat gctttcagta atgcagcagg aagctggtga gtggtttttg ttttgcccgt
    44041 gcgatacgcc ttacattccc cctgatttag cagccaggct taatcatcag cgcaaagatg
    44101 cgcctgtcgt gtgggtccat gacggtgaac gcgatcaccc gactattgct ctggtaaacc
    44161 gcgctattga gcctttatta ctggaatatc tgcaagcagg agaacgccgg gtaatggtat
    44221 ttatgcgtct ggctggcggt catgcggttg atttcagcga tcataaagat gcatttgtta
    44281 acgtgaatac gccagaggag cttgcccgat ggcaggaaaa acgatgatac cgttactcgc
    44341 ctttgccgcg tggagtggta ccggaaaaac tacgcttttg aaaaaactga tcccggcatt
    44401 atgcgccaga gggatccgtc cagggctgat taagcatacg caccatgata tggatgttga
    44461 taagccaggc aaagatagct atgagctgcg caaggctggc gcggcacaaa ccatcgttgc
    44521 cagccagcaa cgatgggcct tgatgacgga aacaccagac gaagaagagc tggatctaca
    44581 gtttctcgca agtcgaatgg atacctcaaa gctggatttg attctggtcg aagggtttaa
    44641 gcatgaagag atcgcaaaga ttgtgctgtt tcgcgatgga gccggacatc gacctgaaga
    44701 attagtgata gacaggcatg ttattgctgt agccagtgat gtgccgctta atcttgatgt
    44761 cgcattactg gatattaatg atgttgaggg gctggctgat tttgtggtgg agtggatgca
    44821 aaagcagaac ggataagcaa gagtaggctg gataagacgc gccagcgtcg catccggcat
    44881 ttttttctgc gccaa
//
LOCUS       DAMZET010000017        44647 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.017, whole genome shotgun sequence.
ACCESSION   DAMZET010000017 DAMZET010000000
VERSION     DAMZET010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.017"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..96)
                     /gene="rpmG"
                     /locus_tag="POB34_001074"
     CDS             complement(<1..96)
                     /gene="rpmG"
                     /locus_tag="POB34_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDI5918064.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPE"
     gene            complement(117..353)
                     /gene="rpmB"
                     /locus_tag="POB34_001075"
     CDS             complement(117..353)
                     /gene="rpmB"
                     /locus_tag="POB34_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDI5918065.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(570..1238)
                     /gene="radC"
                     /locus_tag="POB34_001076"
     CDS             complement(570..1238)
                     /gene="radC"
                     /locus_tag="POB34_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDI5918066.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            1410..2630
                     /gene="coaBC"
                     /locus_tag="POB34_001077"
     CDS             1410..2630
                     /gene="coaBC"
                     /locus_tag="POB34_001077"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDI5918067.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPARVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDEFTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            2608..3066
                     /gene="dut"
                     /locus_tag="POB34_001078"
     CDS             2608..3066
                     /gene="dut"
                     /locus_tag="POB34_001078"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDI5918068.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            3173..3769
                     /gene="slmA"
                     /locus_tag="POB34_001079"
     CDS             3173..3769
                     /gene="slmA"
                     /locus_tag="POB34_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HDI5918069.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(3815..4774)
                     /locus_tag="POB34_001080"
     CDS             complement(3815..4774)
                     /locus_tag="POB34_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HDI5918070.1"
                     /translation="MDATRTTLLALDLFGSPGWSADKEIQRLHALSNHAGRHYRRIIL
                     SKRHGGQRLVLAPDYLLKTVQRNILKNVLSQFPLSPFATAYRPGCPIVSNAQPHCQQP
                     QILKLDIENFFDSISWLQVWRVFRQAQLPRNVVTMLTWICCYNDALPQGAPTSPAISN
                     LVMRRFDERIGEWCQARGITYTRYCDDMTFSGHFNARQVKNKVCGLLAELGLSLNKRK
                     GCLIAACKRQQVTGIVVNHKPQLAREARRALRQEVHLCQKYGVISHLSHRGELDPSGD
                     LHAQATAYLYALQGRINWLLQINPEDEAFQQARESVKRMLVAW"
     gene            complement(5072..5713)
                     /gene="pyrE"
                     /locus_tag="POB34_001081"
     CDS             complement(5072..5713)
                     /gene="pyrE"
                     /locus_tag="POB34_001081"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDI5918071.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(5779..6495)
                     /gene="rph"
                     /locus_tag="POB34_001082"
     CDS             complement(5779..6495)
                     /gene="rph"
                     /locus_tag="POB34_001082"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDI5918072.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            6622..7485
                     /locus_tag="POB34_001083"
     CDS             6622..7485
                     /locus_tag="POB34_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDI5918073.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            7706..8308
                     /gene="dinD"
                     /locus_tag="POB34_001084"
     CDS             7706..8308
                     /gene="dinD"
                     /locus_tag="POB34_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HDI5918074.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQANTTHFD
                     VGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            8599..9216
                     /locus_tag="POB34_001085"
     CDS             8599..9216
                     /locus_tag="POB34_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HDI5918075.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(9213..10730)
                     /gene="ligB"
                     /locus_tag="POB34_001086"
     CDS             complement(9213..10730)
                     /gene="ligB"
                     /locus_tag="POB34_001086"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HDI5918076.1"
                     /translation="MEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTG
                     VRKMVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQK
                     VSLISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTL
                     NSLGVFVWAWPDGPQLMSDRLKELATAGFTLTQTYTRAVKNADEVARVRNEWWKAELP
                     FVTDGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVA
                     SLAPVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTK
                     PTPPENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEH
                     IFSWLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASD
                     ERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(10752..11009)
                     /locus_tag="POB34_001087"
     CDS             complement(10752..11009)
                     /locus_tag="POB34_001087"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918077.1"
                     /translation="MIYCNKDDSQEASSQTPFLPLGFAFTTLYICQPDYSGGEDESMD
                     GDINKYLVLAIICVGGLSGLVASQSTGRNFPPATANKTVGR"
     gene            11149..11772
                     /gene="gmk"
                     /locus_tag="POB34_001088"
     CDS             11149..11772
                     /gene="gmk"
                     /locus_tag="POB34_001088"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDI5918078.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            11827..12102
                     /gene="rpoZ"
                     /locus_tag="POB34_001089"
     CDS             11827..12102
                     /gene="rpoZ"
                     /locus_tag="POB34_001089"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDI5918079.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            12121..14229
                     /gene="spoT"
                     /locus_tag="POB34_001090"
     CDS             12121..14229
                     /gene="spoT"
                     /locus_tag="POB34_001090"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HDI5918080.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            14236..14925
                     /gene="trmH"
                     /locus_tag="POB34_001091"
     CDS             14236..14925
                     /gene="trmH"
                     /locus_tag="POB34_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HDI5918081.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            14931..17012
                     /gene="recG"
                     /locus_tag="POB34_001092"
     CDS             14931..17012
                     /gene="recG"
                     /locus_tag="POB34_001092"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDI5918082.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELQLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(16997..17863)
                     /locus_tag="POB34_001093"
     CDS             complement(16997..17863)
                     /locus_tag="POB34_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918083.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(17866..19071)
                     /gene="gltS"
                     /locus_tag="POB34_001094"
     CDS             complement(17866..19071)
                     /gene="gltS"
                     /locus_tag="POB34_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDI5918084.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            19351..20742
                     /gene="xanP"
                     /locus_tag="POB34_001095"
     CDS             19351..20742
                     /gene="xanP"
                     /locus_tag="POB34_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HDI5918085.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVLSRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            20863..22572
                     /locus_tag="POB34_001096"
     CDS             20863..22572
                     /locus_tag="POB34_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDI5918086.1"
                     /translation="MKFIGKLLLYILIALLVVIAVLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PITGDGNIQLVASGAIQANATLKPTVNGQLHAVNAEKQQVTQTMNAGVVSSSEVTSTE
                     PVQ"
     gene            complement(22624..24942)
                     /gene="yicI"
                     /locus_tag="POB34_001097"
     CDS             complement(22624..24942)
                     /gene="yicI"
                     /locus_tag="POB34_001097"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HDI5918087.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTSKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPIYVRDNTLLALGNNDQRPDYAW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(24952..26334)
                     /locus_tag="POB34_001098"
     CDS             complement(24952..26334)
                     /locus_tag="POB34_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HDI5918088.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            26627..26721
                     /locus_tag="POB34_001099"
     tRNA            26627..26721
                     /locus_tag="POB34_001099"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26661..26663,aa:Sec,seq:tca)
     gene            26800..26928
                     /locus_tag="POB34_001100"
                     /pseudo
     CDS             26800..26928
                     /locus_tag="POB34_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
     gene            27358..28542
                     /locus_tag="POB34_001101"
     CDS             27358..28542
                     /locus_tag="POB34_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HDI5918089.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYDSVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
     gene            28653..29576
                     /locus_tag="POB34_001102"
     CDS             28653..29576
                     /locus_tag="POB34_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDI5918090.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(29580..30398)
                     /gene="nlpA"
                     /locus_tag="POB34_001103"
     CDS             complement(29580..30398)
                     /gene="nlpA"
                     /locus_tag="POB34_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HDI5918091.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKITTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            30620..30913
                     /locus_tag="POB34_001104"
     CDS             30620..30913
                     /locus_tag="POB34_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918092.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(30954..32144)
                     /gene="nepI"
                     /locus_tag="POB34_001105"
     CDS             complement(30954..32144)
                     /gene="nepI"
                     /locus_tag="POB34_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HDI5918093.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFSVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(32355..32807)
                     /locus_tag="POB34_001106"
     CDS             complement(32355..32807)
                     /locus_tag="POB34_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HDI5918094.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVIPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(32860..34194)
                     /gene="adeQ"
                     /locus_tag="POB34_001107"
                     /gene_synonym="yicO"
     CDS             complement(32860..34194)
                     /gene="adeQ"
                     /locus_tag="POB34_001107"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HDI5918095.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDGNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            34369..36135
                     /gene="adeD"
                     /locus_tag="POB34_001108"
     CDS             34369..36135
                     /gene="adeD"
                     /locus_tag="POB34_001108"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDI5918096.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(36180..37571)
                     /gene="uhpT"
                     /locus_tag="POB34_001109"
     CDS             complement(36180..37571)
                     /gene="uhpT"
                     /locus_tag="POB34_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HDI5918097.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(37709..39028)
                     /gene="uhpC"
                     /locus_tag="POB34_001110"
     CDS             complement(37709..39028)
                     /gene="uhpC"
                     /locus_tag="POB34_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HDI5918098.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(39038..40540)
                     /gene="uhpB"
                     /locus_tag="POB34_001111"
     CDS             complement(39038..40540)
                     /gene="uhpB"
                     /locus_tag="POB34_001111"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HDI5918099.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(40540..41130)
                     /gene="uhpA"
                     /locus_tag="POB34_001112"
     CDS             complement(40540..41130)
                     /gene="uhpA"
                     /locus_tag="POB34_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HDI5918100.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(41205..41495)
                     /gene="ilvN"
                     /locus_tag="POB34_001113"
     CDS             complement(41205..41495)
                     /gene="ilvN"
                     /locus_tag="POB34_001113"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDI5918101.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(41499..43187)
                     /gene="ilvB"
                     /locus_tag="POB34_001114"
     CDS             complement(41499..43187)
                     /gene="ilvB"
                     /locus_tag="POB34_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HDI5918102.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(43293..43391)
                     /gene="ivbL"
                     /locus_tag="POB34_001115"
     CDS             complement(43293..43391)
                     /gene="ivbL"
                     /locus_tag="POB34_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HDI5918103.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            43956..44045
                     /gene="tisB"
                     /locus_tag="POB34_001116"
     CDS             43956..44045
                     /gene="tisB"
                     /locus_tag="POB34_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HDI5918104.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            44464..>44647
                     /gene="emrD"
                     /locus_tag="POB34_001117"
     CDS             44464..>44647
                     /gene="emrD"
                     /locus_tag="POB34_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="multidrug efflux protein involved in adaptation to
                     low energy shock; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HDI5918105.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQL"
BASE COUNT        11155 a        12065 c        11088 g        10339 t
ORIGIN      
        1 ttccggctta gtacgtttgt tcttcgtagt ggtatagaag tgaccagtac cagcagaaga
       61 aaccagcttg attttctcac gaataccttt agccatgatt tatttcctct aagtacttag
      121 tacttttcgc cacgggcacg cagttcagcc agaactgtat cgatgccttt tttatcgatt
      181 acacgcatac ctttagcaga tacgcgcagg gtgacaaaac gcttctcgct ctcaacccag
      241 aaacggtgag agtgcaggtt cggcaggaaa cggcgtttag tcgcgttcag tgcgtgggaa
      301 cggttgttac cggtcaccgg acgcttgcca gtaacttggc agactcggga catgtctatt
      361 ctccaaaaat caaattagct cgagcttcgt atggggtatt ggcgcctcgt caggctttac
      421 agcccggtca tcgcagttct atgtgaactc tcgattgcca ggcccaaatg ccaaacccga
      481 gattctcaaa ggtggcgtag tatacgctga ctcagcgatg tgctcaagtc ccgaacagac
      541 aaagatcccg aaggatcgcg catagcgggt taaatccagc cgcgttcggc aaaagaaaca
      601 tactctccac gcccaatcac gatatggtcg agcacgcgta aatccatgaa ctgacaactc
      661 tttattatcc gttcagtaat gagtttatcc gctttactgg gttcagcaca acccgaaggg
      721 tgattatgtg caaggatcag cgccgaggcg tttattttta tcgcttcgcg gataatttcc
      781 cgaggatgga cttcaacatg gtttagcgtg ccggaaaaaa gtcggctatg cgttataacc
      841 cggtgttgtg agtcgagaaa gatcaccata aagatctccc gctcctcacc cgtgagctgg
      901 ctttgtaaaa attcccgcgt catctccggg ctgagtaaag ggctttcttc acgcatccgc
      961 acgttgtagt aacgccgcgc cagttcagca atccccttta actgggcaaa tttcgctacg
     1021 ccaattccat gaacaccact aaattgttca tattcagagg ttaacaagcc ataaagagag
     1081 ccgaaattct ccaacatctc ttttgccagg gttaatacat ctttaccgcg cgttccggta
     1141 cgcagaaata gcgccagcag ctcgacatcc gttaaggcgc taataccaaa cttcagcatt
     1201 ttttcgcgcg gcatcaacag ctgtgcattg tttttcacct tcacctcctt tgtggtgctc
     1261 gcatcctgtc atagccgttc cgttaaatcg acggccagtt ttcaatcctg gaaagcgcct
     1321 cgcaaagtga atctcaggtg aaggcacgac aacaaaagta ttgtgataaa atcgccaact
     1381 tctggtgtca cccaacagga aaaatcatca tgagcctggc cggtaaaaaa atcgttctcg
     1441 gcgttagcgg cggtattgct gcctataaaa cccctgaact ggtgcgtcgt ttgcgcgatc
     1501 gcggggccga cgtccgcgta gccatgaccg aagcggcaaa agcctttatc accccactta
     1561 gtttgcaggc ggtttctggt tatcccgttt ccgacagtct gctggacccg gcagccgaag
     1621 ccgctatggg ccatattgag ctgggtaaat gggctgattt agtgattctc gcccctgcca
     1681 cggcagattt gattgcccgt gttgctgccg gaatggcaaa tgatctggta tcgacgattt
     1741 gtctggctac acctgcgcgt gtagccgtgc tccccgccat gaaccagcag atgtaccgtg
     1801 ccgctgccac gcagcataat ttagaggtgc ttgcctcccg tggtttgctc atctgggggc
     1861 cagacagtgg cagtcaggct tgtggtgata tcggccctgg gcgaatgctc gatccgttaa
     1921 ccattgtgga tatggcggta gcgcattttt cgcccgtcaa cgacctgaaa catctgaaca
     1981 ttatgattac cgccggtccg acgcgtgaac cgctcgatcc ggtgcgttat atctctaatc
     2041 acagctccgg caagatgggt tttgctatcg ctgccgccgc tgcccgtcgt ggcgcgaacg
     2101 tcacgctggt atcaggtcca gtttcactac cgacaccacc gtttgttaaa cgtgttgatg
     2161 tgatgaccgc gctggaaatg gaagccgccg tgaatgcttc tgtacagcag caaaatattt
     2221 ttatcggttg cgccgccgtg gcggattatc gcgcagctac cgtggcccca gaaaaaatca
     2281 aaaagcaggc cacgcagggt gatgaattca caataaaaat ggttaaaaac cccgatatcg
     2341 tcgcaggcgt tgccgcacta aaagaccatc gaccctacgt cgttggattt gccgccgaaa
     2401 caaataatgt ggaagaatac gcccggcaaa aacgtatccg taaaaacctt gatctgatct
     2461 gcgcgaacga tgtttcccag ccaactcaag gatttaacag cgacaacaac gcattacacc
     2521 ttttctggca ggacggagat aaagtcttac cgcttgagcg caaagagctc cttggccaat
     2581 tattactcga cgagatcgtg acccgttatg atgaaaaaaa tcgacgttaa gattctggac
     2641 ccgcgcgttg ggaaggaatt tccgctcccg acttatgcca cctctggctc tgccggactt
     2701 gacctgcgtg cctgtctcga cgacgccgta gaactggctc cgggtgatac tacgctggtt
     2761 ccgaccgggc tggcgattca tattgccgat ccttcactgg cggcaatgat gctgccgcgc
     2821 tccggattgg gacataagca cggtatcgtg cttggtaacc tggtaggatt gatcgattcc
     2881 gactatcagg gccagttgat gatttccgtg tggaaccgtg gtcaggacag cttcaccatt
     2941 caacctggcg aacgcatcgc ccagatgatt tttgttccgg tagtacaggc tgaatttaat
     3001 ctggtggaag atttcgacgc caccgaccgc ggtgaaggcg gctttggtca ctctggtcgt
     3061 cagtaacaca tacgcatccg aataacgtca taacatagcc gcaaacattt cgtttgcggt
     3121 catagcgtgg gtgccgcctg gcaagtgctt attttcaggg gtattttgta acatggcaga
     3181 aaaacaaact gcgaaaagga accgtcgcga ggaaatactt cagtctctgg cgctgatgct
     3241 ggaatccagc gatggaagcc aacgtatcac gacggcaaaa ctggctgcct ctgtcggcgt
     3301 ttccgaagcg gcactatatc gccacttccc cagtaagacc cgcatgttcg atagcctgat
     3361 tgagtttatc gaagatagcc tgattactcg catcaacctg attctgaaag atgagaaaga
     3421 caccacagcg cgcctgcgtc tgattgtgtt gctgcttctc ggttttggtg agcgtaatcc
     3481 tggcctgacc cgcatcctta ccgggcatgc gctaatgttt gaacaggatc gcctgcaagg
     3541 gcgcatcaac cagctgttcg agcgtattga agcgcagctg cgtcaggtat tgcgtgaaaa
     3601 gagaatgcgt gagggtgaag gttacaccac cgatgaaacc ctgctggcaa gccagatcct
     3661 ggccttctgt gaaggtatgc tgtcacgttt tgtccgcagc gagtttaaat accgcccgac
     3721 ggatgatttt gacgcccgct ggccgctaat tgccgcgcag ttgcagtaat tctcccctaa
     3781 acggtcaggc agaaacgtct gcctgacgct tttcttacca tgcaaccagc attcgcttta
     3841 cactctctct cgcctgttga aaggcctcat cctcagggtt gatttgcaat aaccagttta
     3901 ttcttccctg caaagcataa agatacgccg ttgcctgtgc gtggagatcg ccagaaggat
     3961 caagttcacc acgatgacta agatgcgaaa taacgccata tttttggcac aaatgcacct
     4021 cctgacgcag cgcccggcgc gcttcacggg caagctgtgg cttgtgatta acaacaatcc
     4081 cggttacttg ctggcgctta caggcagcta tcaggcagcc tttgcgttta ttgaggctca
     4141 ggcccagctc cgctaacaat ccgcacactt tatttttaac ctggcgggca ttgaagtgac
     4201 ctgaaaaggt catgtcatcg cagtagcggg tgtaggtaat tccccgagcc tgacaccatt
     4261 cccctatgcg ttcatcaaaa cggcgcatca caagattgga aatggctggc gaagttggtg
     4321 ccccctgcgg taacgcgtcg ttataacaac aaatccaggt cagcatggtt accacattac
     4381 gtggcaactg ggcctggcga aacacacgcc agacctgtaa ccagctaatg ctatcgaaaa
     4441 agttttcgat atcgagtttc aggatctgcg gctgttggca gtgtggctgc gcgttgctga
     4501 cgattgggca acctggtcgg taggctgtag caaaagggga aagcggaaat tgtgaaagga
     4561 cgttcttaag aatgttgcgc tgtacggttt tgagcaagta atcaggggct aacaccagcc
     4621 gctgaccacc gtggcgttta gaaagaataa tgcgtcggta atggcgtccg gcatgattac
     4681 tgagcgcatg cagtcgctgt atttctttat cggcgctcca gcccggcgag ccgaacaaat
     4741 cgagcgccag aagggttgtc cgggtagcat ccattccact ctccacatca cggcggccaa
     4801 aagaggcgcc aacagcggca ataccattaa accatcccga aggcgcgtaa ctgtactgag
     4861 cgcgtcagcg cgacgtacgc gaagcgtact caggtacaat gaagcgactt tgggtatatg
     4921 gacatactac tggcgttaag tcagcgactc gccgaccctc ccggcacaaa aggccggaaa
     4981 cgctattgcc actgctggcg aaagcagatt aacacgatat cttgaagcgc atcacgctgc
     5041 gcacagcaaa aaccctgcac agcgcatatt tttaaacgcc gaactcttcg cgataggcct
     5101 taaccgccgc cagatgttcc gccatttccg gcttctcttc caggtaagca atcaggtctt
     5161 tcagggtgat gatagagatc actttgcagt tgtaatcacg ctcaacttcc tgaatcgccg
     5221 aaatctcgcc gcgcccccgt tcctgacgat cgagcgaaat caacacgcca gcaagcgtcg
     5281 cgccattggc ctgaataatt tccatcgact cacgaatcgc cgttccggcg gtgatcacat
     5341 catctaccag cattacgcgt ccttgtaacg cgctaccaac cagattgccg ccttcaccgt
     5401 gatcttttgc ttctttgcgg ttaaagcagt acggcaggtc caggtcatga tgctccgcca
     5461 gcgccacggc ggttgtggtg gcaatcggga tccctttgta agcagggcca aacagcagat
     5521 cgaactcaat gccggaatcc accaacgctt cagcgtaaaa acggcctaac agtgccagat
     5581 cgcgcccggt attaaacagc ccggcgttga agaaataggg gcttttgcgc ccggacttca
     5641 acgtaaactc gccaaacttt aacacctgct tgctaagcgc aaattcaata aactggcgct
     5701 gatatggttt catgccttcg ctcctcatct tacttttcta cagacaaaaa aaaggcgact
     5761 catcagtcgc cttaaaaatc agtttgccag cgccgccttc tgcgtcgcta caatggattc
     5821 gattccccct cgggccagcg ccaacaaggt gagtaactct tcatgggtga acggctcgcc
     5881 ttctgccgtc ccctgcactt caataatgcg cccgtcttcg gtcatcacta cgttcatgtc
     5941 ggtctctgcg gctgagtctt caacgtattc cagatcgcaa attgcttcac cgtttacaat
     6001 accgacagaa accgcggcta ccatcccttt cattggattg gttttcagct tgccgttttc
     6061 caccagcttc tgtagcgcat ctgccagcgc cacacaggca cccgtaatcg aagcggtacg
     6121 cgtgccacca tcagcctgaa gcacgtcgca gtccagcgta atggtgaact cacccagcgc
     6181 tttcaaatct actgccgcgc gaagtgcacg ggcgatcaga cgctggattt ccattgtgcg
     6241 tccaccctgc ttacctttcg ctgcttcacg agcgttacgg gtgtgggtag aacgcggcag
     6301 cataccgtac tctgcggtga tccagccctg gccctgacct ttcaggaagc gcggcacgcc
     6361 ttcttcaata gaggcggtac acaacacttt ggtatcgcca aattcgacca gcaccgagcc
     6421 ttctgcatgt tttgtatagt tacgagtcag ggtaacggga cgcacctgat tattgctacg
     6481 gcctgctgga cgcatattga aatctccggc ttgaaacaaa tgtggctgcg cattatacgg
     6541 acttccggcg gttattccta tcctgacaag gcatcgatgg ctataatcct cccacctctc
     6601 cttttataaa caggaacgtc tatgatccgc agtatgaccg cctacgcccg gcgtgaaatc
     6661 aagggtgaat gggggagcgc aacctgggaa atgcgctcgg taaaccagcg ttatctggaa
     6721 acttactttc gtctgccgga gcagttccgt agccttgaac ctgtcgttcg cgagcgtatt
     6781 cgttctcgcc tgacgcgcgg taaagtggaa tgtaccctgc gctatgagcc agatgttagc
     6841 gcacaaggtg agctgatcct caacgaaaaa ctggctaaac agctggtaac tgccgcgaac
     6901 tgggtaaaaa tgcagagtga cgaaggggaa atcaacccgg ttgatattct acgctggccg
     6961 ggcgtgatgg cagcccagga gcaggatctc gacgcgattg ccgctgaaat tctcgcggcg
     7021 ttggatggta ccctggacga ctttatcgtc gcgcgcgaaa ccgaaggtca ggcgctgaaa
     7081 gcattgatcg agcagcgtct ggaaggcgtc accgccgaag tggtcaaagt ccgcgcccat
     7141 atgccggaaa tcctgcaatg gcagcgtgag cgtctggtcg cgaagctgga agatgctcag
     7201 gttcagttgg aaaacaaccg tctggagcag gagttggttc tgctggcaca acgaattgac
     7261 gttgccgaag aactggaccg cctggaagcg catgtcaaag agacctacaa cattctgaag
     7321 aaaaaagaag ccgttggtcg tcgtctggac tttatgatgc aggaattcaa ccgcgagtcg
     7381 aacactcttg cgtcgaaatc tatcaatgcc gaagtgacaa actccgccat cgagctgaaa
     7441 gtgttgattg agcagatgcg cgagcagatt cagaatattg agtaactcgt aacactaatt
     7501 cttacttttc agccttcaca aatgccgccc actcaaacag agcggcattt ttcttccccg
     7561 caacttcaat tctgttttgc gtgcctgctc cagattttgc gatgtttttt tgcccagcac
     7621 actgagaacg tgagatactc acaactgtat ataaatacag ttacagattt actttctttg
     7681 caatcgatat cacatggagt gggcaatgaa cgaacatcat caaccttttg aagagataaa
     7741 actgattaat gcaaacggag cagaacaatg gtcagcaaga caacttggga aactactggg
     7801 ttattcagag tatcgtcact ttatacctgt attaacgcgc gccaaagagg cctgtgagaa
     7861 cagtggtcac acaattgatg accatttcga ggagatcctc gatatggtca aaattggctc
     7921 aaatgccaaa agagcattaa aagacatcgt actctcccgc tatgcctgtt acctggtagt
     7981 acaaaacggc gaccccgcga aaccggtcat tgcggcaggg cagacttatt ttgctatcca
     8041 gacccgacgg caggagcttg ctgacgatga agcatttaag caacttcgtg aagacgaaaa
     8101 gcgtctgttt ctgagaaatg agttgaaaga acataacaaa caattggttg aggcagcaca
     8161 gcaagctaat accacacact ttgacgtggg tagcaaggtg aggcaaacca ttcaggaact
     8221 tggcggaacc atgcctgagg agttgccgac cccgcaggtc agcatcaagc agttggaaaa
     8281 cagtgtaaaa attacagaga agaaatagca gaaacgaaaa actctttttg ttgattgaga
     8341 cacccgatgc gtaagccaag gtccaggtgc agtgaacaca atggctaaac attgcacctt
     8401 tcttttcccc cctcagtttt aacctatttt ttcttatgca ttttctcaga caagaagtca
     8461 gaagaatgca tctacgctac agaaaatagc gatttcacat aactacaagt tatcaatttc
     8521 ctctcacctt aaaaaatctc aatcgtgaca atacgcacaa atcgctaccc tgccagacag
     8581 atttttaggg agagaaccat gctgttacac attttgtatt tggttggcat tactgccgaa
     8641 gccatgacgg gggctctggc ggccgggcga cgccgcatgg atacatttgg cgtaattatt
     8701 attgcgaccg ccaccgcaat tggcggaggg tcagtgcgcg atattctgct gggccactat
     8761 ccgctcggct gggtcaaaca cccggaatat gtgattatcg tcgctaccgc cgcagtactc
     8821 accactatcg ttgctcctgt aatgccttac ctgcgcaaag tgtttctggt gctcgatgcg
     8881 cttggactgg tggtcttttc tatcatcggc gcacaggttg cactggatat ggggcacggc
     8941 ccaattattg ccgttgtcgc ggcggtaacc accggcgtgt ttggcggcgt tttgcgcgat
     9001 atgttctgta aacgtatccc actggtattc cagaaagagt tgtatgccgg tgtctcattt
     9061 gcctccgccg tgctgtacat cgcgctgcaa cactatgttt ctaaccatga tgtggtaatt
     9121 atctccaccc tggtattcgg cttttttgcc cgcttactgg cgctacgcct taagctggga
     9181 ttaccggtct tttactacag ccacgaagga cactaaggtt caaaacctgt gatctgctgg
     9241 gcagccagcc aactgcccag cttcttgatt tgcgcatttt ccttccattc aataacctgt
     9301 ctggcgcgcc ccgatccagt ccccggcagc tgctgccaga actgctccgt gctaaataaa
     9361 agttgcgacc aggaccgttc atcactggca ttaagcgccg cccgggttag cggtattccc
     9421 attgccatca cccagcgagt aaaaggctgc ttacgagcca gattaaactg atgccatagc
     9481 tgcgcacttt tacttttcgc gatccccggc gtgttctgta attgctctgg cgttaataaa
     9541 agccaggaaa agatatgctc aaagcgatga gtctgatgca gcgcgcgcca gccggcctca
     9601 ccaatgccat ccagccccag aacctgtttt gaccccagcc agactaagcg tgaaatgaac
     9661 tgttcctgac aaacatcaga agcaaagtag caggtcaacg agttaaagcg gttttctggt
     9721 ggtgtcggtt ttgtacgttc tgcaccgcgc cagaccacat catcaatgcg aggaataccc
     9781 tgaccggcaa ggctgacgag gatctgatca ccaggcgcaa tatcccactc ctgccagcgc
     9841 ctgacggaac caatattcac ccgctggact tttttatcat ccagcatgac aggtgcgagt
     9901 gacgcaacca ccgatatttt accgctctta cccaccgcaa actgaatcgc cttcacttcg
     9961 gcaacctgag ctacaggttg atatttccag gccaccagcc attctgcctg gcctggtagc
    10021 caatggcggg attcgggttc tttcgcccct cgcacaacta cgccatcggt gacgaagggt
    10081 aattccgctt tccaccactc attgcgtacg cgcgcaactt catcagcatt tttcaccgca
    10141 cgggtatacg tctgcgttag agtaaaacct gcggtagcca gctcttttaa acgatcagac
    10201 attaactgcg gtccatccgg ccatgcccag acaaaaacgc ccagagaatt cagcgtgtcg
    10261 ctatcgtcct ggcgcatcat caagccagca acttttgcgc gggcatttat tccccccatt
    10321 tgttgttgga tatgcccctc gcgctggaga aatatttccc cctgaagcgt actgttggct
    10381 aaaggcccgc taacggtttg cggcacagcg gaaattaagc taactttctg cgtccagtcc
    10441 tcgcctttca ggccattacc gcgactgatt gctttgttca gtttcccgtc ccgataaacc
    10501 agggttaccg caacgccatc aacttttggc tgcacccaaa gatcgctacg ttctcgcatc
    10561 cacagactta atgcgttttt atccaccatt ttgcgtacac ccgtatgggc aaccggatgc
    10621 ataaccgcac catttaaagg cggcatcatc acgtcgcggg gctcgctccc aaagcagcgt
    10681 tgccactgcg taagacgggc gcttaactga tcgtaaacac cgtcttccac ctcacttttt
    10741 ccttccagta gtcatcgtcc cactgtttta tttgctgttg caggcgggaa atttcttcct
    10801 gtgctctggc tggcgaccag gccggacaga ccgcccacac agatgattgc cagcacaaga
    10861 tacttattaa tatcgccatc catactttca tcttcacctc cgctgtagtc aggctggcag
    10921 atatacaacg tggtgaaggc aaagccgagt ggcaaaaacg gagtctgcga ggacgcttcc
    10981 tgagaatcgt ctttattgca gtaaatcaca ggcaaatgcg gaaacagcta cgcaaaacgc
    11041 aacaactttg cgcaaaaagt gtgagcaagg gctacgtcac atggccgcgc cgtgtataat
    11101 aagctcgtat gtaggcttta tttcgctaat cacatacgaa agatactcat ggctcaaggc
    11161 acgctttata ttgtttctgc ccccagtggc gcgggtaaat ccagcctgat tcaggcttta
    11221 ttaaaaaccc aaccgttgta tgacacccag gtttctgttt cacacaccac acgccaaccg
    11281 cgtcctggtg aagtccacgg tgaacattat ttctttgtta atcatgatga atttaaagaa
    11341 atgattagca gagatgcgtt cctcgaacac gcagaagttt ttggtaatta ctatggcact
    11401 tcgcgtgagg ccattgagca agtactggcg accggtgtcg atgtttttct cgatatcgac
    11461 tggcagggtg cgcagcaaat tcgccagaag atgccgcacg cgcggagtat ctttatttta
    11521 ccgccgtcca aaattgaact ggaccgccgt ctacgcggtc gcggtcagga cagcgaagag
    11581 gtcattgcaa agcgtatggc gcaagctgtt gcagaaatga gccattacgc cgaatatgat
    11641 tatctgattg tgaatgatga cttcgatacc gcgttgaccg atttgaagac cattattcgc
    11701 gccgaacgtc tgcgcatgag ccgccaaaag cagcgtcatg acgctttaat cagcaaattg
    11761 ttggcagact gaacctgatt tcagtatcat gcccagtcat ttcttcacct gtggagcttt
    11821 ttaagtatgg cacgcgtaac tgttcaggac gctgtagaga aaattggtaa ccgttttgac
    11881 ctggtactgg tcgccgcgcg tcgcgctcgt cagatgcagg taggcggaaa ggatccgctg
    11941 gtaccggaag aaaacgataa aaccactgta atcgcgctgc gcgaaatcga agaaggtctg
    12001 atcaacaacc agatcctcga cgttcgcgaa cgccaggaac agcaagagca ggaagccgct
    12061 gaattacaag ccgttaccgc tattgctgaa ggtcgtcgtt aatcacaaag cgggtcgccc
    12121 ttgtatctgt ttgaaagcct gaatcaactg attcaaacct acctgccgga agaccaaatc
    12181 aagcgtctgc ggcaggcgta tctcgttgca cgtgatgctc acgaggggca aacacgttca
    12241 agcggtgaac cctatatcac gcacccggta gcggttgcct gcattctggc cgagatgaaa
    12301 ctcgactatg aaacgctgat ggcggcgctg ctgcatgacg tgattgaaga tactcccgcc
    12361 acctaccagg atatggaaca gctttttggt aaaagcgtcg ccgagctggt agagggggtg
    12421 tcgaaacttg ataaactcaa gttccgcgat aagaaagagg cgcaggccga aaactttcgc
    12481 aagatgatta tggcgatggt gcaggatatc cgcgtcatcc tcatcaaact tgccgaccgt
    12541 acccacaaca tgcgcacgct gggctcactt cgcccggaca aacgtcgccg catcgcccgt
    12601 gaaactctcg aaatttatag cccgctggcg caccgtttag gtatccacca cattaaaacc
    12661 gaactcgaag agctgggttt tgaggcgctg tatcccaatc gttaccgcgt aattaaagaa
    12721 gtggtgaaag ccgcgcgcgg caaccgtaaa gagatgatcc aaaaaatcct ctctgaaatc
    12781 gaagggcgtt tgcaggaagc gggaataccg tgccgcgtca gtggtcgcga aaagcatctt
    12841 tattcgattt actgcaaaat ggtgctcaaa gagcagcgtt ttcactcaat catggacatc
    12901 tacgctttcc gcgtgatcgt caatgattct gacacctgtt atcgcgtgct cgggcaaatg
    12961 catagcctgt acaagccgcg tccgggtcgc gtgaaagact acatcgccat tccaaaagcg
    13021 aacggctatc agtctttgca cacctcgatg atcggcccgc acggtgtgcc ggttgaggtc
    13081 cagatccgta ccgaagatat ggatcagatg gcggagatgg gtgttgccgc gcactgggct
    13141 tataaagagc acggcgaaac cagcaccacc gcgcaaatcc gcgcccagcg ctggatgcaa
    13201 agcctgctgg agctgcaaca gagcgccggt agttcgtttg aatttatcga gagcgttaaa
    13261 tccgatctct tcccggatga gatttacgtt ttcacaccgg aagggcgaat tgtcgagctg
    13321 cctgccggtg caacgcccgt cgacttcgct tatgcagtgc ataccgatat cggtcatgcc
    13381 tgcgtgggcg cacgcgttga ccgccagcct tacccgctgt cgcagccgct taccagcggt
    13441 caaaccgttg aaatcattac cgcaccgggc gctcgcccga atgccgcgtg gctgaacttt
    13501 gtcgttagct caaaagcgcg cgccaaaatt cgtcagttgc tgaaaaacct caagcgtgat
    13561 gattctgtaa gcctgggccg tcgtctgctc aaccatgctt tgggtggtag ccgtaagctg
    13621 aatgaaatcc cgcaggaaaa tattcagcgc gagctggatc gcatgaagct ggcaacgctt
    13681 gacgatctgc tggcagaaat cggacttggt aacgcaatga gcgtggtggt cgcgaaaaat
    13741 ctgcaacatg gggacgcctc cattccaccg gcaacccaaa gccacggaca tctgcccatt
    13801 aaaggtgccg atggcgtgct gatcaccttt gcgaaatgct gccgccctat tcctggcgac
    13861 ccgattatcg cccacgtcag ccccggtaaa ggtctggtga tccaccatga atcctgccgt
    13921 aatatccgtg gctaccagaa agagccagag aagtttatgg ctgtggaatg ggataaagag
    13981 acggcgcagg agttcatcac cgaaatcaag gtggagatgt tcaatcatca gggcgcgctg
    14041 gcaaacctga cggcggcaat taacaccacg acttcgaata ttcaaagttt gaatacggaa
    14101 gagaaagatg gtcgcgtcta cagcgccttt attcgtctga ccgcccgtga ccgtgtgcat
    14161 ctggcgaata tcatgcgcaa aatccgcgtg atgccagacg tgattaaagt cacccgaaac
    14221 cgaaattaat gttttatgaa cccaacacgt tatgcacgca tctgcgaaat gctcgccagg
    14281 cggcagcctg atctgaccgt ctgcatggag caggtccaca aacctcataa cgtttctgcg
    14341 attattcgta ccgcagatgc cgttggcgta catgaagttc acgccgtctg gcctggtagc
    14401 cgcatgcgca ccatggcttc ggcagcggcg ggtagtaaca gctgggtaca ggtgaaaaca
    14461 caccgcacca ttggcgatgc cgtcgctcat ctcaaaggcc agggcatgca gattctggca
    14521 acccatcttt ctgataacgc tgtcgatttc cgcgaaattg attacactcg cccgacctgc
    14581 attttgatgg gacaggagaa aacgggcatc acgcaggaag cattggccct ggcggatcag
    14641 gacatcatca ttccgatgat cggcatggtg cagtcgctga atgtttccgt tgcctcagcc
    14701 ctcattcttt acgaagccca gcgtcagcgg caaaatgcag gcatgtacct gcgtgaaaac
    14761 agcatgttgc cggaagcaga gcaacaacgc ctgttgtttg aaggcggcta tccggtgctg
    14821 gcgaaagtcg caaaacgcaa aggcctgcct tatccccacg tcaatcagca aggcgagatc
    14881 gaagctgatg ccgactggtg ggctactatg caggctgcag ggtaagtgcc atgaaaggtc
    14941 gcctgttaga tgctgtccca ctcagttccc taacgggcgt tggcgcagca cttagtaaca
    15001 aactggcgaa aatcaacctg cataccgtgc aggatctact cttacacctt cccctgcgct
    15061 acgaagatcg cacccatctc taccccatcg gagaactact gccgggcgtt tatgccacgg
    15121 tggaaggcga agtgctgaac tgcaatatct ccttcggcgg tcggcggatg atgacctgcc
    15181 agatcagcga cggttccggc atcctcacca tgcgcttttt caatttcagc gcggcaatga
    15241 aaaatagcct ggcgacgggc cgccgtgtac tggcttatgg cgaagcaaag cgcggtaaat
    15301 atggtgcgga gatgatccac ccggaatacc gcgtgcaggg cgatctcagc acgccagaat
    15361 tacaggaaac gctcacgccg gtttatccaa caacggaagg cgtaaagcag gccacgctgc
    15421 gtaaattaac cgaccaggcg ctggatctgc tcgacacctg cgccattgaa gaactcctgc
    15481 cgccggaact gtcacaagga atgatgacgc taccggaagc gttgcgcact ttgcaccgcc
    15541 cgccgccgac gctacagctt agcgatctgg aaaccgggca gcatccggcg caacgtcgtc
    15601 tgattctgga agaactgctg gcgcacaacc tcagcatgtt agccttacgt gccggagcac
    15661 agcgttttca tgcccagccg ctgagcgcca atgacgcgct gaaaaataaa ctcctcgccg
    15721 ccttaccgtt caagccaacg ggcgcacagg cacgcgtagt ggcggagatc gagcatgata
    15781 tggcgctgga tgttccaatg atgcgcctgg tgcagggcga tgtaggttcc ggtaaaacac
    15841 tggtcgccgc cctcgccgcg ctgcgtgcga ttgcccacgg caaacaggtg gccctgatgg
    15901 caccaaccga attactcgcc gagcagcacg ccaataactt ccgcaactgg tttgcaccgc
    15961 tcggtatcga agtgggctgg ctcgccggta agcagaaagg taaagcgcga ctggcacagc
    16021 aggaagccat cgccagcggt caggtgcaga tgattgtcgg tacacacgcc atcttccagg
    16081 aacaggtgca gtttaacggt ctggcgctgg tgattatcga cgaacagcat cgttttggcg
    16141 tgcatcagcg tctggcattg tgggagaaag gccagcagca gggcttccat ccgcatcagt
    16201 tgatcatgac cgccacgccg atcccccgca cgctggcaat gacggcgtat gccgatctcg
    16261 atacctcggt gatagatgag ctgccgccag gccgcacgcc agtgactacg gtcgctattc
    16321 ctgatacccg ccgtaccgac atcattgacc gcgtgcgcca cgcctgcatg actgaaggtc
    16381 gtcaggcgta ctgggtttgt acgttgattg aagagtcaga gttactggaa gcgcaggcgg
    16441 cggaagctac ctgggaagag ttgaaactgg cgctaccaga attgaatgtt ggcctggtac
    16501 acgggcggat gaaaccagcc gagaaacagg cggtgatggc gtcgtttaaa caaggtgagc
    16561 tacaactgct ggttgccact accgttattg aagtcggcgt tgatgtgcct aacgccagtc
    16621 tgatgattat cgaaaaccct gagcgtctgg gtctagcgca gttacaccag ctgcgcgggc
    16681 gcgtaggtcg tggcgcggtg gcttctcact gcgtgctgct ctacaaaacg ccgctctcca
    16741 aaacggcgca aattcgcctg caagtgctgc gcgacagtaa cgacggtttt gtgattgcgc
    16801 aaaaagatct ggagattcgc ggccctggcg aattgttagg cacgcgtcag acgggtaatg
    16861 ctgaatttaa agtggcggat ttactgcgcg atcaggcgat gatcccggaa gttcagcgcc
    16921 tggcgcgaca tattcacgaa cgttacccac aacaagcaaa agccctgata gaacgctgga
    16981 tgccggagac ggaacgttac tccaacgcat gaccagactc atcacaagac aagtcacgca
    17041 gtaaatcttc aatctggata gtgaggatat ccattgcctc atacgcatcc tgtgcaattc
    17101 gcgccgccat tgcgccttcc ggttcaccac gcagaatttc agccgctgcc tgtacgccag
    17161 ccacaatcat ttcaggtgtt aacatttggc gaagaaaagc gcgattatca gaggaccagc
    17221 catctttatg gcctaatgga tgcccccagc ctatccattc ttgcgaccat aacggattgg
    17281 cttctatggg ataaattaga tgcgcggcat actgaatagc acacaccgct tttcctggcg
    17341 tatccagcgc ccaccagcgc gaccagagag aacgaattaa cggcgcggcc ccacccagag
    17401 tattgaacac cgccagccag caaagaacgt tattgaaacc acgctcgtta tccatacgct
    17461 gcaacgcggt gtccagtaaa aattgccgta cctgctggcg ttgggaagca ttcatcatct
    17521 cctgccagag atagggccga cacaatgcct tcattaagga ttcttcgaag tgatcaccat
    17581 aaccgccagc taaaacggtt tcatgccatt gcgccaggca gagcggaaac agatagcgaa
    17641 acaactctat ttgcatcggt tcgttataaa cgagattgag tacatagtaa gcactcaaat
    17701 cccattcgct gatctgctgc cactctttag ccgccagcgc cttgagttcg gcatctgcgt
    17761 aatcaaattc cgtttcccat acacagagca ctggcggctc atcaccaaaa atatggcgac
    17821 agtgttcacg tagcgtttcc ggttccttcc gacgcttttt catttttaac cggcaaaaat
    17881 cggcaacatt aaatacagct taatcaccag cgcattgacg atatcgataa agaacgcacc
    17941 gaccatcggc accaccaaaa acgccatgtg cgacgggcca aagcgttcag tgatcgcctg
    18001 catgttggcg attgccgttg gcgttgcacc gaggccaaaa ccacagtgac ccgcagccag
    18061 cactgccgca tcgtagtttt tgcccatcat gcgccaggta acgaagatgg catacaacgc
    18121 catgaagatg gtctgtacca ccagaatcgc cagcatcggc agcgccagcg aagccagctc
    18181 ccacagtttc agccccatca gcgccatcgc caggaacaac gacaggctta cgttacccag
    18241 cacggatacc gcacgctcaa agacgcggta aaagcccatc attgacagac cgttgctcag
    18301 aatcacgcca acaaacagga cacagacgaa ggtcggcagt tcaaaagcag tgccagccaa
    18361 aagttgcgca acaattttcc ccaccgtcag gcagatagca atcagcgcga tagtttcaat
    18421 cagcaccagc gaggtgatca tgcggcccac atccggcttt tcaaacgccg tcgggacttc
    18481 ctggtcatcc ggaataccgt tcggcgtggt ggagtgtttc accagatagc gcgccaccgg
    18541 accgccaatc aagccgccca gcaccagacc gaacgttgca caggccatcg ccacttccgt
    18601 cgcattggtg aagccataac gttcaatgaa caatttactc cacgcagcgc ccgtaccgtg
    18661 accgccggaa agcgtaatag aaccggccaa cagccccatc agcggatcaa gccctaacag
    18721 gctagccata ccaatgccaa tggcattttg catcaccaac agaccaacaa ccacaatcaa
    18781 gaagatgcca accacacgcc caccggcacg caaactggca atgttggcgt tcaggccaat
    18841 ggtggcgaag aaagccagca ttaacggatc gcgcagggac atatcaaagt tgacttccca
    18901 gcccatgctt tttttcagta ctagtagcgc cagcgccacc aacaaaccac ccgcaacagg
    18961 ttccggtatg gtgtatttct tcaaaaagga gacggaatgg accaacttac gcccgagcag
    19021 caacgtcagc gttgcggcaa caagcgttgc taaagtatcg agatgaaaca tagttactcc
    19081 tttgtatccg catcactctt tcatactcgt catacttcat cgcgcagtgg caacttcatg
    19141 aactatgacg ggtatcgagt tatgctttcc tgggtcaaat tctcagctgt taatgaacaa
    19201 atggcatgga ttttaagcag aaaaatacca aaagttatat aaaaaagtgc atttatctca
    19261 tttaaatatt tagatgagga tgtggcaaac gtttgctttt ctctttcggt cagctaaaat
    19321 gcccgctttg ctaccacggg attgttttcg atgtctgttt ccaccctcga gtcagaaaat
    19381 gcgcaaccgg ttgcgcagac tcaaaacagc gaactgattt accgtcttga agatcgtccg
    19441 ccgcttcctc aaaccctgtt tgccgcctgt cagcatctgc tggcgatgtt cgttgcggtg
    19501 atcacgccag cgctattaat ctgccaggcg ctgggtttac cggcacaaga cacgcaacac
    19561 attattagta tgtcgctgtt tgcctccggt gtggcatcga ttattcaaat taaggcctgg
    19621 ggtccggttg gctccgggct gttgtctatt cagggcacca gcttcaactt tgttgccccg
    19681 ctgattatgg gcggtaccgc gctgaaaacc ggtggtgctg atgttcctac catgatggcg
    19741 gctttgttcg gcacgttgat gctggcaagt tgcaccgaga tggtgctctc ccgcgttctg
    19801 catctggcgc gccgcattat tacgccgctg gtttctggcg ttgtggtgat gattatcggc
    19861 ctgtcgctaa ttcaggttgg gttaacgtcc attggcggcg gttacgcagc catgagcgat
    19921 aacaccttcg gcgcaccgaa aaatctgctg ctggcaggcg tggtcttagc cttaattatc
    19981 ctgcttaacc gtcaacgtaa cccttactta cgcgtggcct cactggtgat tgcgatggcg
    20041 gccggatatg cgctggcgtg gtttatgggc atgttgccag aaagcaacga accgatgacg
    20101 caagaactga ttatggtgcc aacgccgctc tattacggtc ttggcattga atggagtctg
    20161 ctgctgccgc tgatgctggt ctttatgatc acttcgctgg aaaccattgg cgatatcacg
    20221 gcgacctctg acgtttccga acagccagtg tccggtccgc tgtacatgaa acgcctgaaa
    20281 ggcggcgtgc tggcaaacgg cctgaactcg tttgtttcgg cggtgtttaa caccttcccg
    20341 aactcctgct tcgggcaaaa caacggagtg atccagttga ctggtgttgc cagccgctat
    20401 gtcggttttg tcgtcgcgct gatgttgatc gtgctgggtc tgttcccggc agtgagcggt
    20461 tttgtacaac acattccaga accggttctg ggcggcgcaa cgcttgtaat gtttggcacc
    20521 atcgccgcct ccggtgtgcg tatcgtttct cgtgagccgc tgaaccgtcg ggcgattctg
    20581 attatcgcgc tgtcgctggc ggttggtctg ggcgtgtctc agcagccgct gattttgcag
    20641 tttgcccctg aatggctgaa aaacctgctc tcctccggga tcgccgcggg cggtattact
    20701 gccatcgtgc tgaatctgat tttcccacca gaaaaacagt aattcactgg cggcggtaag
    20761 ataattaccg ccgcagtctc acctctttca ccattcccgc cttgaggatt gcgcgtaaat
    20821 cgtgcataac tcccttatgt gcatttcacg ggatggaaga ccatgaaatt tattgggaag
    20881 ctgcttctct acattctcat cgctctgtta gtggtgatcg ctgtcctcta ttttcttctg
    20941 caaacccgct ggggagcaga acatatcagc gcatgggttt ccgagaatag cgactatcat
    21001 ctggccttcg gggcgatgga tcaccgtttt tccgcgccat ctcatatcgt gctggagaac
    21061 gtcacgtttg gtcgtgatgg tcagcccgcg accctggtgg caaaaagtgt cgacattgcg
    21121 ctaagcagtc ggcaactgac cgaaccacgc catgtcgata ccatcctgct ggaaaacggg
    21181 acgctgaatc tcaccgacca gaccgcgccg ctaccgttca aagccgatcg tctgcaactg
    21241 cgtgatatgg cgtttaatag cccgaatagc gaatggaaac tgagcgcgca gcgggtaaat
    21301 ggcggcgtgg ttccgtggtc accagaagcc ggtaaagtgc tgggtacgaa ggcgcagatt
    21361 cagtttagtg ccggatcgct ttcgctcaat gatgttcctg ccaccaatgt actgattgaa
    21421 ggcagtattg ataatgatcg cgttacgctg actaacctgg gtgccgacat cgcccgcggg
    21481 acattaaccg gaaacgcgca gcgtaacgcc gacggcagct ggcaagtgga aaacctgcgc
    21541 atggcggata tacgtctaca aagcgaaaaa tcgctaaccg acttctttgc gccattacgc
    21601 tctgtcccgt cgttgcagat tggtcgcctg gaagtgatcg atgctcgttt gcaaggtccg
    21661 gactgggcgg tgaccgacct cgatctcagc ttgcgcaaca tgaccttcag taaagatgac
    21721 tggcagacac aggaaggcaa actgtcgatg aacgctagcg agttcattta tggttcgctg
    21781 catttatttg acccgattat aaacgcggaa ttttccccgc agggcgtagc gctgcgccag
    21841 ttcaccagcc gctgggaagg gggtatggtc agaacgtcag ggaactggct gcgtgacggg
    21901 aaaacgttga tccttgatga tgcggcaatt gccgggctgg aatatacctt gccgaaaaac
    21961 tggcaacagt tgtggatgga aacgacaccc ggttggttaa acagcctgca actgaagaga
    22021 tttagcgcca gccgcaatct gatcattgat atcgaccctg acttcccgtg gcagctcacc
    22081 gcgctcgatg gttacggtgc caacctgacg ctggttaccg atcataaatg gggcgtctgg
    22141 agtggctcgg cgaatctgaa tgccgccgcc gcgacattca atcgtgttga tgttcgtcgc
    22201 ccgtcgctgg cgctgaccgc caacagcagc acggtgaata tcagcgaact gagtgcattt
    22261 actgaaaaag gcattctgga agccaccgcc agcgtatccc aaacgccaca acgtcagacc
    22321 catatcagtc tgaacggacg cggtgttccg gtgaatattc tgcaacagtg gggatggcct
    22381 gagttaccca taactggcga tggcaatatt caactggttg ccagcggtgc gattcaggcc
    22441 aatgccacgc tgaaacctac cgttaacggg caactccacg ccgtaaatgc tgaaaagcag
    22501 caagtgactc aaaccatgaa tgcgggcgtc gtttccagta gcgaagttac atcgacagag
    22561 ccggtgcagt aaacatcaat ctcgccgggt acgactcaga cgtacccggc attcttcaat
    22621 agattacaac gtaattgtca gcgcattccc ttgaggcgtc accaccagcc cctgctcact
    22681 ttcagcctgc gaaccgcctt gcagaccatt tactttcaca acattgcgca ggcacagtgt
    22741 ccagttcttc gcctcgcccg caccagtcac agtaatcgtg ttgccagtac gtgctgcttt
    22801 tagagtaaag atcaccgaac cgtcagcagc gggcacttca cagacggctt catgcccgtc
    22861 ttgcagatta aagaggtgga atgccgtgcc ttcgtgccac gcgtaatcgg gacgttgatc
    22921 gttgttgccc agcgccagta gggtgttatc acgcacataa atgggcagac tcaggaagct
    22981 gtgctgctgt ttatgccagc gactaccgtc gagctcatcg ttgtgccaca ggtgtgtcca
    23041 gcgaccttct ggcaggtaga actgcacatc gcccgcttca gtgaacaccg gagcaaccat
    23101 cacgttgtcg cctaacatgt attgacggtc aaggtaatca caagccggat cgtccgggaa
    23161 ctccatcatc atggcccgca tcatcggcgt accccgcgcg tttgcacgcg cagcttcacg
    23221 atacagatac ggcatcatgc ggcatttcag ttgcgtgaag aagcgcacca catcacagga
    23281 ctcatcatca taggcccacg gcacacgata agatttactg ccatgtaaac ggctatggct
    23341 ggagagcaaa ccaaacgcgc accagcgttt gtaaacgtgt gccggagcgg tattttcaaa
    23401 gccgccgata tcgtggctcc agaagccaaa acctgaaagg ccaatagaca aaccaccgcg
    23461 caggctttcc gccattgatt cgtagttagc gtaacaatcg ccaccccagt gtaccgggaa
    23521 tttctgcgca ccgacggagg ccgagcgggc aaacaagaca gcttcttcct caccaacggt
    23581 gtccttgagc acgttccaca ccagttcgtt gtagatgtac gcataatggt tatgcatttt
    23641 ctgcggatcg gaaccgtcaa accactgaac atcagttggg atacgttcgc caaagtcggt
    23701 cttaaagcaa tcaacgccca tcgcgaccag acctttcagt ttgtcggcgt accatttgca
    23761 agcatccgga ttggtaaagt cataaatcgc cagacctggc tgccatttat cccactgcca
    23821 tagcgaaccg tccgggcgtt tgagtaaata gcctttctct tgtaactctt taaagacggg
    23881 ggatttttga ccgatatagg ggttaatcca gacgcagatt ttcagccctt tcgctttcag
    23941 gcggcggatc atcccttccg ggtcagggaa agtcagcggg tcccactcaa aatcgcacca
    24001 ctggaaggct ttcatccaga aacagtcaaa gtggaaaaca tgcagcggca gattgcgttc
    24061 cgccatacca tcgataaagc tgtttaccgt cgcttcgtcg tagttggtgg taaatgaagt
    24121 ggttagccac aggccgaagg accacgcggg cggcagcgcc ggacgaccag taaagcgggt
    24181 ataacgatca agtaccgctt tcgacgtcgg gccgtcgata acaaagtatt cgagatattc
    24241 actctcaacg ctgaactgca ctttggagac tttctccgat cccacttcaa aagagacaca
    24301 ctggggatga ttgaccagta ccccataacc acggttagtc atgtagaacg ggatattttt
    24361 atacgcctgt tcagtacttg tgccgccgtc ccggttccag gtctctaccg tctggccatt
    24421 gcgcaccagg gcagtaaagc gctctcccag accgtaaact gtttcgccaa cgccaagatc
    24481 aagccgctca aacatataat tgcgttgatt attcgtgtcc tgcacgtagc cattattttt
    24541 cacctgacta ccggtaatac gttcgccgtt gcgcagaaaa tccagtgacc agaactcacc
    24601 tttgctgaca cgcgcgctta agttgccact tttaaactca gcataacgtt ctgtgttttc
    24661 gattgtgacc ttcacgtcct gcaaaatatt gagcggataa tgaggaccgt tattcagcgc
    24721 cccctgaaaa tgctcaatcc gcacaccgac aataccttcc tgtggggaga aaaagcgcag
    24781 cgtaaataaa ggcgtatcaa gctgccaggt acgttcacgc acatcacggg gggcagcata
    24841 gaccaccatt tcattaccct gctgttcaac ctcgaatacc tgaagcgggt gaatcaaatt
    24901 gaggccaggt tgaatcaacc agtttccatc gctaattttc attgccgttc cttagttctg
    24961 cacttcttga gaggtgaatt gttgctggca acggcgttta ccctgagcca gctgttccat
    25021 aacggttttc agattgtgcg tcgtgagtga gtagtagcgt ttagcgataa tcgcgctcag
    25081 caaataacag atcgccggaa caatcgtgaa tagcgcaata atgatgctaa tcgtggcgct
    25141 gttctgcgct ttttctgccg catcatatcc gccataagcc agcatccagc cgataagagc
    25201 gccgccgaag gccaacccca gtttgagcac aaacagcgtg ccagcaaaac tgatcccggt
    25261 caggcgttta ccattgcacc actcgccgta gtcgacggta tcggacatca ttacccactg
    25321 gataggtgtc accagttgat gcaacacacc aatcacgaag atgaagacaa acatagtgat
    25381 gctggcctgc atgggaacaa agaacatcgc gaggctaatc actgccagca gggcgttcgt
    25441 ccaccagaag atagtgactt tacatttcca gtcggtcaga ggttttgcca gtgcggaacc
    25501 aatcaggtta cccacgcaat aagtggtgag aaaagcgaca aacacttccg gcgtgcccaa
    25561 aatccatgtg acgtaataca tcatcgcccc accgcgtacg cacaccgcca ggatattgaa
    25621 aatggttagt aaaccgacaa tccgccactg gtcgttttgc cagatatcac gtaaatcttc
    25681 ccgcatagac gttgttgtag gtggtgcttc aacgcgttct ttagtggtga agaaacaaaa
    25741 tgccagcatc atgaatgcca ccacggaaag gaccgcgata ccgccctgga aaccgagtgg
    25801 tttattatca ccgccaatta aattaaccag tggcatcatc agaacagtag aaagcatgcc
    25861 tcccgcggtc gccagcacaa aacgccagga ttgcagcgag atacgctgag tcgggtcatt
    25921 ggtgattaca ccacccaatg cgcagtaagg gatattgacg acggtataaa gtaaggtaag
    25981 tagggtgtaa gtaattgctg catagatcat tttgccgttc atactgagat ctggcgtgct
    26041 ataggccagt acacagacga tcccgaatgg cagtgcgcca aacagtaccc acggacgaaa
    26101 tttaccccag cgagagcgcg ttcgatcggc caacaacccc atgcaaggat cggaaatcgc
    26161 atccagtgca cgagcgacca aaaacatggt tccgacaaaa ccggcaggaa tgccaaaaat
    26221 atcggtataa aagaacatca tatataacat tacgttatcg aaaataatgt ggctggcggc
    26281 gtctcccatg ccataaccaa ttttctcttt aacggacaac acttcactct tcatctgctt
    26341 ttccttgatg ctataggggt gctgagaccg ataacgcatt ttgtaaacca tccctgtgag
    26401 agcgaatatt ccgatatctg gttattaatt tatgattctt gttttatgtg atcgtggtag
    26461 cgttaattcc gctcatatat cattgtaaaa tatgggtttt atatgaacta taatgctttc
    26521 gtgataatac gctgcgtgta ttaggcggaa aaaactgatc tgggggatgt agaaactcaa
    26581 ggaagtagct ataatgcgcc ccgcctccat gtagcaatcg aggcgcggaa gatcgtcgtc
    26641 tccggtgagg cggctggact tcaaatccag ttggggccgc cagcggtccc gggcaggttc
    26701 gactcctgtg atcttccgcc aaaatgcctc tcctgacgtt ctccaacccc ttgtaatact
    26761 gtcaatccag atattttcta ctgctcaata ttcaatcggc ttcaggaata cctggtctaa
    26821 ggttttgtga tggacgatga gcggctgaag aatccgcctg tcggttcttc tgtcctcgaa
    26881 cgcatccgcg atatgcgcgc cagcatgtgc cgtgtttatt tgcaggtgat ttgccccctg
    26941 ctggctctca gccaactgta tcgccattaa ggtgaagatg ttgttttact cgccccagaa
    27001 cacccttttt tatacgacta ttctattcct ttaacagctg gcgaaaaaaa ctgacctata
    27061 aacttcacca ataatcagac tgccatcaag attaatttgt tcatttaata tatcaaatca
    27121 acttgtttta tttttactat caaaaatagc tcattgcctt actataatta atatataaat
    27181 tcggtgatta attcttaaca tgctttttct aataatctca attgctggcc ctataatatt
    27241 gcgctagcat tgttttctgg ttgtatcagc gatactcaaa aattcttaat acaatactca
    27301 ttcgactggt acttatttgt aactcagtta tattttgtcg cccggtgatt cagaagaatg
    27361 caaaaaacgg ctaccactcc atcaaaaata cttgacctca ctgccgcggc atttttactt
    27421 gtcgcctttc tgacgggtat tgcgggcgct cttcagactc ctaccctaag tatattcctc
    27481 gcagatgaac tgaaagcccg tcctataatg gtaggttttt tcttcaccgg tagcgctatt
    27541 atgggaattc tggtcagtca atttctggca aggcactccg ataaacaagg cgaccgtaaa
    27601 ttactgattc tgctatgttg cttatttgga gtgctggcct gcacgctttt tgcgtggaat
    27661 cgcaactact tcattctcct ctcaacgggc gtacttctga gtagttttgc ttccaccgca
    27721 aacccgcaaa tgttcgccct cgcccgtgaa cacgccgaca gaacaggccg tgagacggtt
    27781 atgttcagta catttttacg tgctcagatc tcgcttgcct gggttatcgg gccaccgctt
    27841 gcttatgaac tggcaatggg atttagtttt aaagtgatgt atctcaccgc tgccatcgca
    27901 tttgttgttt gcggactgat agtctggttg tttttgccat caatacaaag aaatattcct
    27961 gtcgttaccc aacccgtaga aattttaccc tccacccaca ggaagcggga tacgcggcta
    28021 ctttttgtgg tctgttcaat gatgtgggcg gcgaataatc tctacatgat aaatatgccg
    28081 ctatttatta ttgatgaact gcatctaacc gataaactgg ctggagaaat gattggtatc
    28141 gctgccggtc tggaaattcc gatgatgtta atcgcaggct attacatgaa acgtattggc
    28201 aagcggctat taatgctcat tgctatcgtg agtggaatgt gtttttacga cagcgtactc
    28261 atggcgacga ctccggcggt tgagctggaa ttgcaaattc ttaatgccat cttccttggt
    28321 attctctgtg gtatcggcat gctttatttt caggacttga tgcctgaaaa aataggctct
    28381 gcgacaacgt tatatgcaaa tacttcacgc gtcggctgga ttatcgccgg ctctgttgac
    28441 ggaattatgg ttgaaatctg gagctaccat gcgttgttct ggctggcgat agggatgttg
    28501 ggtattgcga tgatttgcct gctgtttatt aaagatattt agctcaccag tctgggctaa
    28561 gaaaaatcgg cgtggcgcaa acagagccat cctcgccatt tttgctatca tgcctgcata
    28621 cataaacgac aaaacagtat gcagagggaa aaatgggttc caccagaaag gggatgctga
    28681 acgttctgat tgccgccgtg ttgtggggaa gttcaggggt ctgcgcgcaa tacatcatgg
    28741 agcaaagcca gatgtcgtcg cagtttttga ctatgacgcg tttgatattc gccggtttga
    28801 ttctactgac gctgtcattt gttcatggcg ataaaatctt ttctattatt aacaatcata
    28861 aagatgccat tagcctgctg attttttccg tggttggcgc gctaactgta cagctcactt
    28921 ttttgctaac catcgaaaaa tcgaacgcag ccacggcaac ggtgctgcaa ttcctctcac
    28981 cgacgattat cgtcgcctgg ttctcactgg tgcgtaaatc gcgcccgggc attctggttt
    29041 tctgcgctat tttgacatcg ctggtcggga cttttttatt ggtgacacac ggtaatccga
    29101 cgtcattatc gatctctcct gccgcgttgt tctggggcat tgcctcggca tttgctgctg
    29161 cattctatac cacctatccc tcaacgctaa ttgcccgcta tggcacgtta ccagtcgtcg
    29221 gctggagtat gctgattggc ggtctgattc tgttgccttt ttatgccaga caaggaacaa
    29281 actttgtcgt taacggcagt ttgattctgg cgttttttta tttggtggtc attggtacgt
    29341 ccctgacatt tagtctgtac ctgaaaggag cacaattaat tggcggtcca aaagccagca
    29401 ttttgagctg tgcagaacca ttaagtagcg cgctactctc tttgctgttg ctggggatca
    29461 cgttcacatt accggactgg ctgggaacgc tgctgattct gtcatcggtg attttgattt
    29521 caatggattc ccgtcgccgc gccagaaaaa taaatcgtcc ggcgcggcat gagtgagaat
    29581 taccagccag gcaccgcgcc accgttaaaa atggtttctg ccgctttagc gacttcgggt
    29641 gattgataag attgcagaaa ttccttcacg ttttctgcat tcttattatc ttcccgtgcc
    29701 accaaaatat tcacatacgg cgaattctta tcttcaataa atacgctgtc gtgcaccgga
    29761 gaaagccctg tctgctgaat gtaagtggta ctgataatcg ctacatcaac tttgggatca
    29821 tccagtacgc gcggaagctg cgccccctcg agttccataa tctgcaaatg acgaggatta
    29881 tcggttatat ccagcgcggt aggtaataac ccttttccct ctttcagagt aattagcttc
    29941 tctttttgca acagtaaaag tgcgcgacca aggttggtcg ggtcgttggg gatcgccact
    30001 gtcgcgcctt cttttatttg cgccacggtt gtgatttttt tggaataacc cgccataggg
    30061 aagacaaacg tatttcccac cgctaccagt ttataaccat gcgcctgatt atcttgttca
    30121 agaaagggtc gatgctggaa gacattcgca tcaagttcgc catgatttgt cgcatcgttc
    30181 ggtagcaacg aaccgctaaa tcctaccagt tcaacatcga ggccatattt ctcttttgcc
    30241 acctttttag cgacttccgc gacatcttgt tctgcgccat ttataacgcc aactttaatg
    30301 tgttttgcat cgctgctact ctggtcgcaa cctgccagca gaattccggc cagcaataat
    30361 gcggcccctg tccgtagatg atgtgttgtc agtttcattt ttttatcctt ttattaatga
    30421 ttacctgact atagcggtaa ggtcgctgcg gtttaaaaaa cgaaacgcta tcgataagaa
    30481 taaaaaggaa taaaagtgga atataaagtc tggcacttct tactgaccac gcaagcgcgt
    30541 ttcgtacagc atgatgagag cgatgaaagc aaactacact tatgcttcat tcgatacaca
    30601 tttgtaaaag gttgaatcca tgaaaccaac gacactactg cttattttca cttttttcgc
    30661 tatgccaggc atcgtatacg ccgagtcgcc ttttagctca ctgcagtccg caaaagagaa
    30721 aaccacggta ttacaagatt tacgtaaaat ttgcacgcca caggcgtcat tatcagatga
    30781 agcgtgggaa aagttaatgt tgtcagatga aaacaataaa caacatatac gcgaggccat
    30841 cgtggcgatg gaacgtaata atcagagcaa ttattgggaa gcgttgggta aggtagaatg
    30901 cccggatatg taagaacagc aacaactact gaccaacgct tctttacctg atatcaggat
    30961 ttcttcattt tcacctttgc agtaaccaac aatgcagtca gcaacatcaa tgtgccggac
    31021 aacatcaacg gcgaagtcag accaatatta tccagcgcat aaccgccgat tgccgcgcca
    31081 caggtattag caagctgaat aaccgccacc tgaatagacc cggctttttc tgcctgatcg
    31141 gccagcgagc gggtgatcca cgttgaccag ccgacaggaa ccaatgcaaa agttagcccc
    31201 cagataatcg ccacgccggt agcaacgatt ttatcgcttc cccacaacgt cagcaccaac
    31261 gcactcacag ccagtattaa cggcgcgcct gctaaggcca gttttaccga acgtttaaga
    31321 atgaatgacg aaagcgacgt accgataaag ctggcaatgc caaaactcaa cagcaccagc
    31381 gttaagccat ccacgctgaa tcccgccagg ttcatataca ccgggcgaat atatgtgaag
    31441 aaagcaaact gcccggcgaa agacatgaag atggcgatca tccctgccat cacacccgga
    31501 cgttgtaata agcggaaagt attttgtttc tgatgcgagg gttcgcctgg cagtgaaggc
    31561 aatgatttga taatccagaa aatacacagc acgcccatca ccgccgccgc attaaagaca
    31621 ttgcgccaac cgataagctc gcctaaaaaa ctgcccaacg gcgcggcaat caccagcgca
    31681 atagaaaccg cgccgaagat caccgacagc gccttcggca ccgtacgcgg cggcaccaga
    31741 cgcatggtca gcgacgccga catcgcccag aacccgccca gcgccagccc cagacaggca
    31801 cgaccgatta aaagcaaact gaatgagtta gcaaaggaaa ccagcaagca ggagagcgtc
    31861 agcaaaacgg caaacaaaat aacaacgtag cggcggtcag tagcctgaat tgtctgggta
    31921 ataaacaaac tggcaaacat tgccacaaag gcggtcacgg tcaccgattg cccggcaacc
    31981 ccttccgaaa tgcctaaatc ctgggccatt ggcgtcaaca aactgacggg caaaaactca
    32041 acgataatca gacaggcgac acaaaacgcc accgagaaaa cggctgacca gttcggtcgg
    32101 gtgatggcat ccgcgccgcg gttttcggca ataaattcac tcatggtgtt acccgtggca
    32161 tgtttcaggg gaaaagccga ccactttaac attgaaatgt gtgacgcatt taacgttttt
    32221 gcaactttta cggtgttgct tgagctggag ttaagatcga aacggaacag ggcaaagatt
    32281 accggatgcg gcgtgaacgt cttatccacc cgacaggtca ggcgcggtct gtaggctgat
    32341 aagacgaaac ggtttcaatg caaccagtga acgccctcgg caggctgaaa gaacgcgtta
    32401 tagcgcatct gaaaagcatt aatatcctgc atttcaggtt cggtttcgcg cagaaaacct
    32461 agcgccagcc tgacctgagc gtcggtaatc ggcgcggcga ggcgagcttt ttgtaaaatc
    32521 cgatgccatt gcagcaggtt ctgttcgcta ccatcgacaa tgacaatttg ccagatgagc
    32581 aacacctgcg cggcgttttg caggtgcgac tcatccggac gatgcaaata acgtaaaaat
    32641 tcgtcaccag ctgactttcc catttgatcg accatcgact ccacgggtat gacatcgata
    32701 ttcagccgat cgctgagacg gcgaatcgct tttctggccc cggatgtcag cacccgaaaa
    32761 ccaaccacaa acaccactgc cagcgtggcg agcattatcc agatcatgag ttctcctgcg
    32821 taatcagaac tcatgatatc tggaaaccct cgccagttac taatccacca gaataatctt
    32881 cagtgcaaac agagctgcga ccaccaccac acacaggttc agatcgcgcc agcgcccggt
    32941 gcacactttc atgatgcagt acgacataaa gccgagtgca atcccttcgg tgatcgagaa
    33001 agtaaagggc atcatcaccg tggtaataaa cgcaggcacc gattcggtaa aatcatccca
    33061 gttaacgcgc gccaggctcg aagtcatcag cacgccaaca aagattaacg ctccggcggt
    33121 tgcgtaagga ggaaccatcg ccaccagcgg tgagaagaac ataaccaaca ggaacataac
    33181 gccaaccaca accgcagtca gccccgtgcg gccaccgact gccacaccag aagtactttc
    33241 aatataggca gtaacagacg aggtgccgat aaacgcaccc gccaccgaac tgacgctatc
    33301 aacatacagc gccttattca tattggggaa tttaccgtta ccatctatta agcccgcttt
    33361 atcagttaca ccaattaatg ttcctgatga atcaaatagg ttgatcagca taaaggagaa
    33421 aatgataccg gcgagttcaa gtgttaacgc gccgctcaaa tctacttcac caatgacgcc
    33481 gctaatatca ggcggaatgg aatagacgcc gctaaaatga acatcaccga aaaataatcc
    33541 acagcaagac gtcaccacaa tagaaaccag caccgcggca tgaaaatgac gtgatgacaa
    33601 cacggttata ataaaaaacc ctaaaatacc taacaacacg ccgtgagaac ttaaatcgcc
    33661 aatcatcacc agcgtgtctt tattggcgac aataacgcca gtatttttta atcccattaa
    33721 ggcgataaat aatccaattc cgctggtgat accaatacgt aaacttaagg gaatgttgga
    33781 gatcatccag taccggatac gaaacagcgt gagcaaaaat agtccaactg cgccccagaa
    33841 tattgcgccc atcccggtct gccaggagat gcccatcgcc cccacgacca cgaaggcaaa
    33901 gaaggcgttc agccccattg ccggagccag cgccacgggt aagttagcaa atatccccat
    33961 cgcaatactg ccgataccgg caatcaaaca ggtggtaaca aacaccactt tcgggtccat
    34021 ttgtgccgcg ccgaggattt gcgggttcac aaaaacgatg tacaccatgg ttaaaaaagt
    34081 ggtcatcccc gcaatcaatt ctgtgcggac ggtggtccca tgctgaggta gtttaaataa
    34141 tcgcgaaagc gtccctgatt cattactcac gtaatcggta ttgtcattat tcattttttt
    34201 gtccattgga ggagatttaa tcccttgctt ttgtaaaaca aatgacaaca acggttcagt
    34261 gataattatc acatttcaat tgcacattaa tggatattct ttaataatct cgcgacgttt
    34321 ctttatgata aataataatc aaattgataa aatcaaaatg agaaaaatat gaataattct
    34381 attaaccata aatttcatca cattagccgg gctgaatacc aggaattgtt agccgtttcc
    34441 cgtggcgacg ctgttgccga ttatattatt gataatgtct ctattctcga cctgatcaat
    34501 ggcggagaaa tttccggccc aattgtgatt aaaggacgtt acattgccgg tgttggcgca
    34561 gaatacgctg atgctccggc tttgcagcgg attgatgctc gcggcgcaac ggcggtgcca
    34621 gggtttattg atgctcacct gcatattgaa tccagcatga tgacgccggt cacttttgaa
    34681 accgctaccc tgccgcgcgg cctgacgacc gttatttgcg accctcatga aatcgtcaac
    34741 gtgatgggag aagccggatt cgcctggttt gcccgctgtg ccgaacaggc aaggcaaaac
    34801 cagtacttac aggtcagctc ttgcgtaccc gccctggaag gctgcgatgt taacggtgcc
    34861 agttttaccc ttgaacagat gctcgcctgg cgggaccatc cgcaggttac cggccttgca
    34921 gaaatgatgg actaccctgg cgtaattagc gggcagaatg cgctgctcga taaactggat
    34981 gcatttcgcc acctgacgct ggacggtcac tgcccgggtt tgggtggtaa agaacttaac
    35041 gcctatatta ctgcgggtat tgaaaactgc cacgaaagtt atcagctgga agaaggacgc
    35101 cggaaattac aactcggcat gtcgttgatg atccgcgaag ggtccgctgc ccgcaatctc
    35161 aacgcgctgg caccgttgat caacgaattt aacagcccgc aatgcatgct ctgtaccgat
    35221 gaccgtaacc cgtgggagat cgcccatgaa ggacacatcg atgccttaat tcgccgcctg
    35281 atcgaacaac acaatgtgcc gctgcatgtg gcatatcgcg tcgccagctg gtcgacggcg
    35341 cgccactttg gtctgaatca cctcggctta ctggcacccg gcaagcaggc cgatatcgtc
    35401 ctgttgagcg atgcgcgtaa ggtcacggtg cagcaggtac tagtgaaagg cgagccgatt
    35461 gatgcgcaaa ccttacaggc ggaagagtcg gcgagactgg cacaatccgc tccgccatat
    35521 ggcaacacca ttgcccgcca gccagtttcc gccagcgact ttgccctgca atttacgccc
    35581 ggaaaacgct atcgggtcat tgacgtcatc cataacgaat tgattacgca ctcccactcc
    35641 agcgtctaca gcgaaaatgg ttttgatcgc gatgatgtga gctttattgc cgtacttgag
    35701 cgttacgggc aacggctggc tccggcttgt ggtttgcttg gcggctttgg actgaatgaa
    35761 ggtgcgctgg ctgcgacggt cagccatgac agccataata ttgtggtgat cggtcgcagt
    35821 gccgaagaga tggcgctggc ggtcaatcag gtgattcagg atggcggcgg gctgtgcgtg
    35881 gtacgtaacg gccaggtcca aagtcatctg ccgttgccca ttgccggcct gatgagcacc
    35941 gacacggcgc agtcgctggc ggaacagatt gacgccttga aagccgccgc ccgtgaatgc
    36001 ggtccgttac ccgatgagcc gtttattcag atggcgtttc tttctctgcc agtgatcccc
    36061 gcgctaaaac taaccagtca ggggctattt gatggcgaga agtttgcctt cactacgctg
    36121 gaagtcacgg aataataaaa aagcccggcg tcatgccggg caaaagtcac cagttacgtt
    36181 tatgccactg tcaactgctg aatttttttc tcgcggcgga ttttgcgttc ttccatcacc
    36241 gccactatcg ccatcaggca gatacaacca atcgcggcga tatcaagtgc ggcgaatgtg
    36301 cctgcccagc cggtaaggcc gaataccggc gtcccatcgg caatcattcc cagacctaac
    36361 ttggcaaagc tgtcaccaat caggtaagca aaggtgcctt taataccatc ggcagcgcca
    36421 atcgcttttt taggtacaaa gccaacagca gccacaccaa tcaacaattg cgggccaaag
    36481 accaggaaac ccaacgcaaa gagagaagcc agatagatat attcgttgct ggcatgttga
    36541 tacacaccga gcgtggcgat aatcagcgcc agcgcgatgc aggccaccag gccacggcga
    36601 ccgttcgcca ggtcagagag ccagccccac agcagcgtac cgaccagcgc accagcttca
    36661 aacagcgtaa agccctgaat cgccaccgct ttagagagtt tcagttcctg gaacgcgtat
    36721 acggttgacc actggtcgat accaatacgt accacataga ggaaaatgtt ggcgaagcac
    36781 agcagccaga tcactttgtt tttcagcaca tactcaacaa agatctgcca cttggtcata
    36841 tcggtagatt ctgtctcttt gtcctcttcg ctgatctcct cgccgaacag ttcttcagct
    36901 ttgccgaggc cataagattc cggggagtcg ctaccgtaac gcaggccgat aaaaccgaca
    36961 atcagcgcga taatcgacgg gaagataaac atgccgatga catggccatc gaacaggtaa
    37021 tttgccccga acagcgccac acctgctgcg cctgcaccgc caaggttgtg agaaatattc
    37081 cagaaaccga ggaatgtccc gcgtttacga cgcggcgtcc atttggtgat ggtggagtaa
    37141 ctgcacgaac cgccggtact ctggaaaaag ccgcttaagg cgtagaaggc aatcatcagg
    37201 aacaggctaa ccgagccgct gcccatactg gcactgaagc ccagcataca aatggcagac
    37261 aggataagca taaacggcag gaattgtttg gtgtttttgc cgtcggcgta gtaggaaacc
    37321 agcgttttac ccacgccata agtgatggag aaacccaggc cgatcatccc cagctgcgtc
    37381 atgctcaacc cgtaggtcga aatcatgtcg ttctgcgcga tgttaaagtt cttgcgaatc
    37441 aggtacatcg tcaggtagcc gataaagacc accaggtagg attgcatgaa cggtttgaac
    37501 cacattttgc gccgcacttc gagcggaagg tccagggtcg gcttgcgaac ctggtttaag
    37561 aaagccagca tgggttactc ctgaaatgaa tacctgcgtg aggcatgcat tgtaaaaatc
    37621 agcagcaaat caggcctgag atagcgtcca ggcaaaacct ggaaaatttc ttagttttgc
    37681 cggatatgaa gtgaaaaggt gagatgcatc acgcttcgcg cggtgtctgg gcgttcaaaa
    37741 agggcagtaa cagcagtgcg gaaatcccgg cggcgataga gataaccaca aaaaatccgc
    37801 tccagtgcca ggtatcgagt actttcgcca gcggccaacc agcaagcgac gcccccagat
    37861 aagcaaacaa gccgacaaac cccgtcgccg cccctgccgc ctctttgtgg gaacactctg
    37921 ccgccgccat accgattaac atctgtgggc caaagacaaa aaaaccaatg gtgaagaagc
    37981 aggttgcctg catcacgtag ctggcaaatg gcatcagcca cagggagccg actgaaagca
    38041 aaattccggc ggcgaaaatc aaattcatcg gccctcggtt gccgttaaac aatttgtccg
    38101 agccccaacc cgctaccagc gcaccgataa atccacccag ttcaaacatc gtcactgccg
    38161 tattcgccgt gaccagatcg acgcccagcg tctcggacat atacaaattg ccccagtcgt
    38221 tgatcgccgc ccggaccaca tagaccagca catagcaaaa cgaaagcagc cagatatacg
    38281 gattcagcaa cacatatttg gtgaggatct ctttacgcgt cagccctgcc ccttcttgtt
    38341 gttgagctat ctccagcgcg tcgtgtcgcc attcaccgac cgccggtaaa cctaacgcct
    38401 gcgggcgatc gcgcagccgc cagcagagaa aaattcccac gacgatcgcc atacaaccag
    38461 caatcatcat cccggcacgc cagccgtaat gtagtgcagc cgctgccatc acaatgggaa
    38521 tgagtgcgcc gccgacgtta tgcgccgtgt tccataatgc ccaccaaccg ccgcgctcgg
    38581 tacgtgaata ccaggccgtt aacagacgcg cacacaccgg tgaaccccag ccctggaaaa
    38641 aggcgttcag cacccagagc acggcaaacg cccatagcga cgtcgagaag ccaaacagaa
    38701 tgttgataat gcccgtggcg ataagcccta tccccataaa ataacgggca tttgagcgat
    38761 cgctgacaat gccggagaca aacttcgaca cgccataggt aatgtaaaac agggtcgcta
    38821 acaggccgat atcgctacgg ctgagcacgc cgttagcaag gatttctggt acggcggcgt
    38881 taaaactttt ccgcgtgaag taaaacagcg cataacccag ccagatggtc agcagaatat
    38941 gccgacgcca gtagcgatag cgggcatcaa tttcatattt atcagtcatt aatggcgcat
    39001 cggcaggcgc tttcagaaac ggcaacatca tcaaacctta gacatagcgt tgaggtagag
    39061 aaacgctgac acgcgtgccg tgcagacagg aaatgtgtaa tgtgccaccc agcgccgtta
    39121 cgcgctcgcg cattccggtg aggccaaaac cttgttgccc ggaacccggc ggcaaaccgc
    39181 tgccatcgtc ttcaataacc agcatcaacc gttcatcctg ctgccagcct tgcagcgtga
    39241 ccgcgctggc atcagcatgt ttcacaatgt tgttcagccc ttcctggcag acacgaaaca
    39301 gcgtcacgcg ctggttttca cttaatgctg attcatcgat tcgccattcg agatggctga
    39361 caataccgcg cccttccagc tccatttccc gcatcagtga gcggatggcc tgctccaggg
    39421 tgagatcatc caactggcgc ggacgtaacc gacccaacaa acggcgcacc gcgtcgtaaa
    39481 cgcccagcga tagttgttcg atgagctgcc cgctctgctt cacgctggcg ttatctgccg
    39541 ccagccgctg aacaatgccc gcctgagtac gaatagcagt gatggtctga ccgatatcat
    39601 catgcagctc acgcgccaca tcacggcgca cgctctcttc ggtttccagc aaccgttcag
    39661 ccagatgctg attgcgcgcc agttcctttt gcagcgactg gttaagttca cgcaaccgct
    39721 ggatgccagc gccaagcaac aaccctgtca gactttgcac cagcagcgag agcaataaat
    39781 ccaccggatg atcgcgccag gtttgactgg cgatcagcgc gatggcgttc atcaacgtcg
    39841 caatcagcgc cccttgccaa ccatagtgcc aggccagcgc gataatcggc agcgccagac
    39901 agaatggcgt aaagcgcgac agttcgtccg gcaatcccaa ctggagccag agactgataa
    39961 caaacagcag caagtaccag accagatgtc gcccgcgcca gttgattggc tgagaaacca
    40021 gtgacgggcc gagcggcagc caggtgttat tggcgagata gtgccagaac accagacata
    40081 tcggggccag cgtcaggccg ccagttaaag tcagcaacag cgcattccac gactctttgc
    40141 cgtgccaaag ccagggcagc gactgcaaca acgccgccgc cgttaacgcc gccccctgta
    40201 acagcaaggt gcgccagtca cgctgatggc gatagcgcga gatcagcgct acgggcagta
    40261 acgtcagtaa actaccgatc atcaataacg gaaaatgggt taaaccgacc gcctgcgtta
    40321 gccagtaaat cagcagccac tccgcaccca gcaatacggg ccagtatccg cgcgggcatt
    40381 gcagcattag ccccagacgc agaccaaacg gaaataacag caccgccata tcagggcgct
    40441 caaccagatg caggctgata ctccacaggc aaaaccatgc ggcagagaag ataaaaaagc
    40501 aggcaataac ggtaattaag cgggagaaca acgtcttcat caccagccat caaacatgcg
    40561 gcgcgccagc tctacgtcgt tactgacacc cagtttttcc atcaggttgg cgcgatggac
    40621 gtgcaccgtt ttcggcgaca agcccagttc ggcagcaatc tctttcaccg ccattccttg
    40681 cgccagtttt tccgccacct gccgttcgcg tttggttagc gggtcctgac gaccggatgc
    40741 cagtttaatg gcaatatccg gcgtcagata acagccgccc gtggcaaccg tatgcaccgc
    40801 cgcaatcagt tcgtcagggc tacagcgttt ggagagaaag ccgcgtgccc ccgcgttaag
    40861 cgcctgctca accagcgccg gactgtcatg aacagagagc attattgtcg ccataccttt
    40921 cggcagctgg cttagcagtt ccagaccgga gatatcgggc atggagatat cgcaaataca
    40981 cacctgcaca ccgcgccccg gcagccccgc cagcgcctcg cgccccgaac caaactcggc
    41041 aactacctgc aaatcaggtt ccagccccag cagctgcgca aagccggagc ggacgatgag
    41101 gtgatcgtct ataagggcaa cggtgatcat ggtcttgtcc tggcgggtaa aaaaaaacgc
    41161 gcttacctta acgataagcg cgatgttgtt caagccttga gcggttactg aaaaaacacc
    41221 gcaatcttgt taaacatagt cggatcggac tgattacgct gcactttcac cacatcttcc
    41281 agcttatcga tttggcttat catctgctcc agacgctggt cgtcattgac cagtagccag
    41341 atatggcttt tgtcgctgtc ctgaatcggc agacaaagaa tgccttcaac gttaaaagcg
    41401 cggcgggcaa aaaggccaca aacgtgggtc attacgcccg gatggttgcg aacggtgagc
    41461 tccagaatca cgttgtcatg agttgtgttt tgcatggctt attcccccac catttcagta
    41521 ttcgccgcac ctggcggcac catcgggtaa actttttctt cggcatcaat gcgcacatgg
    41581 atcagcgccg ggccagggcg attgatgatt tcctgcaatg cagcctgcgg atcggcttcg
    41641 ttattcaaat cacaggtttc gaggccgaat ccggcggcaa tctgcataaa gttgattttt
    41701 cccggatagg tggcggcaaa aacgccttgc tcgtagaaca gactctgttg ctgatgcacc
    41761 agccccagcg cttcgttgtt catcagaatg attttgacat ccagctgatt ttcactggcg
    41821 gtcgccatct cctgaatatt catcatcagg ctgccgtcgc cggagaaaca caacactttg
    41881 cgatccgggt tcgccagcgc cgcgccaatc gccgcaggca ggccaaaacc catcgtgccc
    41941 agcccaccgg aggtcagcca ctggcgtggg cgattgagcg gataagcttg cgcggtccac
    42001 atctgatgct gacccacatc ggtggtgata atcgcattgt catcgacaca ggcggcaacg
    42061 gcgttgatca ggccgtaatg gcttaatgga tcgcacgctt tcgggattgg acacgggaat
    42121 tcgcgctgca aatccgctac caactggtgc cactctgcac gcggttgcgc ttccacctgc
    42181 gggatcaatt gcgccagcac gtcatcaaca tccgcctgaa tcgccacatg cggctgcttg
    42241 attttaccca gctctgcgcg atcgatatcg acatgaataa ttttggcatt cggacagaac
    42301 tgctcggttt tgccaatcgc ccggtcatca aaacgcgcac cgagcactat caacaaatcc
    42361 gcctcctgca aaatatagtt ggtgctgcgc acgccgtgca tccccagcat acccagcgac
    42421 aacggatgcg cttttggcaa catgcccagc gccattaaag tcatggtggt aggcagttgc
    42481 gctttctccg ccagttcacg cacccgtgcg ggcgcattga tcacaccgcc gcccagataa
    42541 agcaccgggc gtttggcagc gttaatcatc gccgctgcgt cacgaatgct ttcttcgcta
    42601 aaggcggggg cggcggcttt ttctgccata gcgggctgtg tttcaatctc aaaaactgcc
    42661 gtttgcacat ccttaggaat gtctatccac accgggcctg ggcggcctga ttgcgcaatg
    42721 cggaaggcat cgctcatgac ctgcgggagt tcttcgatat gtctgaccag atagttgtgt
    42781 ttggtgatgg ggatagagat gccgtaggtg tccacttcct ggaaggcgtc ggtgccgatc
    42841 atcgaggcgg gaacctgacc ggtgatgcaa atcagcggga tggagtccag ccgcgcatcg
    42901 gcaatggcgg tcaccaggtt agtcgcaccc ggtccgctac aggccataca gaccgccggt
    42961 ttaccgtcgg tgcgcgccat cccctgagcg ataaatcccg cgccctgttc atgacgggcc
    43021 agaatatggc ggatttgcgt gctttggctt aaggcatcgt aaacaggcag gatagaaccg
    43081 cccggaatgc ccgtcacaat cttaatgccc tgctgttcca ggaaatgaac gataaattct
    43141 gcgccggtaa agcgcttacg cgtcgatgtt gtgcccgaac ttgccatgct ccagtccttt
    43201 tcttctgggc cgactttccg ggaggtgctt aaacgaaaaa ccccgcccgg tttgcgccgg
    43261 cggggttttg gaatcgtgtg ttgttccagt ccctacggcg cattgccgac gaccaccacc
    43321 acacgcacga cgaccactgc ggcggatagc gcagttggta gtagttttgc gttgaacatg
    43381 gaagtagtca ttggggacct tgttggtttt gtgtttaaca atatttatac aagcacagct
    43441 ttacagggga gacaatggaa aatttttcag caagggaaaa ttgaggggct gatcacgttt
    43501 tgtactgaat tgcaggtaac aaaaaacccc gccggagcga ggtttcatca gccgcctgcg
    43561 gctggtaacc gcaaagcaca ctgtattatg tcaacactga aagtatacgt gttccgcgca
    43621 gaacgcgcaa tttcggcacg aattttgacg tatttagggc atagttgagt atcgatcaca
    43681 gtttgcgttt tgtccaaata ttactgttta tttatacagt aaacttctat aatatcactg
    43741 tacgcaatgt gttatgcggg ggccgcatcg ttacccggcg cactaagtcc tggctgaaac
    43801 gggtggtgcc gtcagcgcct taaccccgcg tgagcacact gtgttatgtc aacaagcaca
    43861 acgtttctcc ttaagatacc gcgtgcacaa cagctggcaa caggcagcgg aaaggtacgt
    43921 cagctggcag tgctcctgaa ccacaggaga cgcgtatgag cctggtggat atcgccattc
    43981 ttatcctcaa actcattgtt gcagcactgc aactgcttga tgctgttctg aaatacctga
    44041 agtaattcag attcaagtcg caccaaaggg gagcgggtaa ccgctcccct tttatattta
    44101 gcgtgcgggt tggagttggg ggcttcttct accagcagaa tgtttgcacc tgcgctgcct
    44161 gtgcccctca ccctagccct ctccccaaag gggcgagggg accgtcccgt gcaatgttta
    44221 gctcattttc tgaaagggaa cggtggtttc cccctcgccc ctttggggag agggttacag
    44281 ttcgcacgta atccctcccg gcaacattta ttgccgcttt tgtttacata ttctgccgct
    44341 aaacaattcc ccattcctgg cgtatatctg gctaacattc atcaatgtga tagattcctc
    44401 tcccgcattt atgggaatgc gtagtgactt attctaatta tttttataaa agcatccgtg
    44461 ataatgaaaa ggcaaagaaa cgtcaatttg ttattgatgt tggtattact cgtggccgtc
    44521 ggtcagatgg cgcaaaccat ttatattcca gctattgccg atatggcgcg cgatctcaac
    44581 gtccgtgaag gggcggtgca gagcgtaatg ggcgcttatc tgctgactta cggtgtctca
    44641 cagctgt
//
LOCUS       DAMZET010000018        44250 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.018, whole genome shotgun sequence.
ACCESSION   DAMZET010000018 DAMZET010000000
VERSION     DAMZET010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.018"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..217
                     /locus_tag="POB34_001118"
     CDS             <1..217
                     /locus_tag="POB34_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDI5918106.1"
                     /translation="SVPAFTLIAGDRSVSGSATGTPHELRKLMRFAARSKVAPTTELF
                     PMSKINDAIQHVRDGKARYRVVLKADF"
     gene            complement(221..784)
                     /gene="idnK"
                     /locus_tag="POB34_001119"
     CDS             complement(221..784)
                     /gene="idnK"
                     /locus_tag="POB34_001119"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDI5918107.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            1001..2032
                     /gene="idnD"
                     /locus_tag="POB34_001120"
     CDS             1001..2032
                     /gene="idnD"
                     /locus_tag="POB34_001120"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HDI5918108.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTEMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGASEIVCADVSPRSLLLAKEMGADVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            2056..2820
                     /gene="idnO"
                     /locus_tag="POB34_001121"
     CDS             2056..2820
                     /gene="idnO"
                     /locus_tag="POB34_001121"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HDI5918109.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAE
                     RAELAVEKLRQEGIRAVAAPFNVTHKHEIDTAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            2885..4204
                     /gene="idnT"
                     /locus_tag="POB34_001122"
     CDS             2885..4204
                     /gene="idnT"
                     /locus_tag="POB34_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HDI5918110.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLLPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            4271..5269
                     /gene="idnR"
                     /locus_tag="POB34_001123"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             4271..5269
                     /gene="idnR"
                     /locus_tag="POB34_001123"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HDI5918111.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSEHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSATPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            5347..6849
                     /locus_tag="POB34_001124"
     CDS             5347..6849
                     /locus_tag="POB34_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HDI5918112.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGVAEEGTSSEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRSDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASTEQQNNPAVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGVLGSLLGGRRR"
     gene            complement(7010..8092)
                     /gene="lptG"
                     /locus_tag="POB34_001125"
     CDS             complement(7010..8092)
                     /gene="lptG"
                     /locus_tag="POB34_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDI5918113.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(8092..9192)
                     /gene="lptF"
                     /locus_tag="POB34_001126"
     CDS             complement(8092..9192)
                     /gene="lptF"
                     /locus_tag="POB34_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDI5918114.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            9459..10970
                     /gene="pepA"
                     /locus_tag="POB34_001127"
     CDS             9459..10970
                     /gene="pepA"
                     /locus_tag="POB34_001127"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDI5918115.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            11231..11674
                     /gene="holC"
                     /locus_tag="POB34_001128"
     CDS             11231..11674
                     /gene="holC"
                     /locus_tag="POB34_001128"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HDI5918116.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSNPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            11674..14529
                     /gene="valS"
                     /locus_tag="POB34_001129"
     CDS             11674..14529
                     /gene="valS"
                     /locus_tag="POB34_001129"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDI5918117.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVKNGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDDAGNVYVGRN
                     EDEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(14583..15779)
                     /locus_tag="POB34_001130"
     CDS             complement(14583..15779)
                     /locus_tag="POB34_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HDI5918118.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRHQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAVGMGTV
                     FFISTKMLHANSSSSVIISMVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            15972..16475
                     /locus_tag="POB34_001131"
     CDS             15972..16475
                     /locus_tag="POB34_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5918119.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYDGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMDHAHEMGFKRCYLETTAFLTEAIALYEHLGFQHIDYALGCTGHVDCE
                     VRMLRDL"
     gene            complement(16522..16938)
                     /gene="rraB"
                     /locus_tag="POB34_001132"
     CDS             complement(16522..16938)
                     /gene="rraB"
                     /locus_tag="POB34_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HDI5918120.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDVVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEEYIDDDDDGVRH"
     gene            17100..18104
                     /gene="argF"
                     /locus_tag="POB34_001133"
     CDS             17100..18104
                     /gene="argF"
                     /locus_tag="POB34_001133"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDI5918121.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(18162..19181)
                     /locus_tag="POB34_001134"
     CDS             complement(18162..19181)
                     /locus_tag="POB34_001134"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918122.1"
                     /translation="MNWQAQDYKDFFKDALATTLSTIKQEHLLIMPHVCNEIIPDLLG
                     EEGILEEIDNLSASDDAFTNNKKSNDEINKIKIDLAHILIDSLDDAPVDLSPVIRSML
                     ETFLKSPYMSDVKILEWCFNKSIEYFNAAAKIDYACDVIKNIKSGCDQSKRAEALFSL
                     LDKEPYKNSCKLQGLIWDKLVAYVNDFNLSNQEKSRLILKLFDDVKLLFNEVPVSILV
                     NDIFLKGFFMKQPDFAKWYFYQLLKCYEGEQLYLNELGYVYGDEEKTKEIVNKLPGYV
                     VKIFEEKMVNELKIRTRMMEVLRDGKINIYNYINEKQLEKLNPPENLRAVIEKWGWKN
                     RTHTA"
     gene            complement(19182..19760)
                     /locus_tag="POB34_001135"
     CDS             complement(19182..19760)
                     /locus_tag="POB34_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918123.1"
                     /translation="MSKISHSDYFHYVASCRNFKPKHDVLNSWCVGINNFSRIAEGQH
                     NKRNILSPKAFLEFLAWIFTLGHVDFSESRNEAGRKMMGNIKSSSYLKKDDGSEIMRF
                     YMNNPEGWEDDIRPVILEVRLTPPSVNGARQGRTAIIFDDVTESGKSIFRFEGTDFET
                     KDESSLLLITNKVLACYQREINNKIALQLNNN"
     gene            complement(19883..20335)
                     /gene="tabA"
                     /locus_tag="POB34_001136"
     CDS             complement(19883..20335)
                     /gene="tabA"
                     /locus_tag="POB34_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HDI5918124.1"
                     /translation="MIIGNIHNLQSWLPQELRQAIEHIKANVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            complement(20480..21073)
                     /locus_tag="POB34_001137"
     CDS             complement(20480..21073)
                     /locus_tag="POB34_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDI5918125.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAISAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLVDILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGGAIKTILKE"
     gene            21144..21818
                     /gene="bdcA"
                     /locus_tag="POB34_001138"
     CDS             21144..21818
                     /gene="bdcA"
                     /locus_tag="POB34_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI5918126.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     KRLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGEALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMARGLARDFGPRGITINV
                     VQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAGPEASFVTGAMHTI
                     DGAFGA"
     gene            21949..22344
                     /locus_tag="POB34_001139"
     CDS             21949..22344
                     /locus_tag="POB34_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDI5918127.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            22625..22759
                     /gene="pyrL"
                     /locus_tag="POB34_001140"
     CDS             22625..22759
                     /gene="pyrL"
                     /locus_tag="POB34_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HDI5918128.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            22763..23698
                     /gene="pyrB"
                     /locus_tag="POB34_001141"
     CDS             22763..23698
                     /gene="pyrB"
                     /locus_tag="POB34_001141"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDI5918129.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            23711..24172
                     /gene="pyrI"
                     /locus_tag="POB34_001142"
     CDS             23711..24172
                     /gene="pyrI"
                     /locus_tag="POB34_001142"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HDI5918130.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            24245..24631
                     /gene="ridA"
                     /locus_tag="POB34_001143"
     CDS             24245..24631
                     /gene="ridA"
                     /locus_tag="POB34_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HDI5918131.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(24837..27533)
                     /gene="mgtA"
                     /locus_tag="POB34_001144"
     CDS             complement(24837..27533)
                     /gene="mgtA"
                     /locus_tag="POB34_001144"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDI5918132.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(27674..27727)
                     /gene="mgtL"
                     /locus_tag="POB34_001145"
     CDS             complement(27674..27727)
                     /gene="mgtL"
                     /locus_tag="POB34_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HDI5918133.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            27912..28859
                     /gene="treR"
                     /locus_tag="POB34_001146"
     CDS             27912..28859
                     /gene="treR"
                     /locus_tag="POB34_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HDI5918134.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            28978..30399
                     /gene="treB"
                     /locus_tag="POB34_001147"
     CDS             28978..30399
                     /gene="treB"
                     /locus_tag="POB34_001147"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HDI5918135.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILTVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYHFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            30449..32104
                     /gene="treC"
                     /locus_tag="POB34_001148"
     CDS             30449..32104
                     /gene="treC"
                     /locus_tag="POB34_001148"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDI5918136.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALT
                     DESSVFYTYQKLIALRKQEAVLTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRG
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            32498..34636
                     /gene="nrdD"
                     /locus_tag="POB34_001149"
     CDS             32498..34636
                     /gene="nrdD"
                     /locus_tag="POB34_001149"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDI5918137.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDKWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            34795..35259
                     /gene="nrdG"
                     /locus_tag="POB34_001150"
     CDS             34795..35259
                     /gene="nrdG"
                     /locus_tag="POB34_001150"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HDI5918138.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(35304..35690)
                     /gene="cybC"
                     /locus_tag="POB34_001151"
     CDS             complement(35304..35690)
                     /gene="cybC"
                     /locus_tag="POB34_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HDI5918139.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(35745..37097)
                     /gene="pmbA"
                     /locus_tag="POB34_001152"
     CDS             complement(35745..37097)
                     /gene="pmbA"
                     /locus_tag="POB34_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HDI5918140.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            37191..37742
                     /locus_tag="POB34_001153"
     CDS             37191..37742
                     /locus_tag="POB34_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HDI5918141.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(37898..39271)
                     /gene="mpl"
                     /locus_tag="POB34_001154"
     CDS             complement(37898..39271)
                     /gene="mpl"
                     /locus_tag="POB34_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HDI5918142.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            39447..40445
                     /gene="fbp"
                     /locus_tag="POB34_001155"
     CDS             39447..40445
                     /gene="fbp"
                     /locus_tag="POB34_001155"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HDI5918143.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(40478..41473)
                     /gene="yjfF"
                     /locus_tag="POB34_001156"
     CDS             complement(40478..41473)
                     /gene="yjfF"
                     /locus_tag="POB34_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HDI5918144.1"
                     /translation="MIKRNLPLMITIGVFVLGYFYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(41460..42482)
                     /locus_tag="POB34_001157"
     CDS             complement(41460..42482)
                     /locus_tag="POB34_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5918145.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMSQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(42496..43998)
                     /locus_tag="POB34_001158"
     CDS             complement(42496..43998)
                     /locus_tag="POB34_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDI5918146.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGFLRRKEMEKRATELMTSYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
BASE COUNT        11163 a        11410 c        11080 g        10597 t
ORIGIN      
        1 gtctgttccg gcctttacgt taattgcggg cgaccgcagc gtctctggtt cagcaaccgg
       61 tacacctcat gagctgcgta agctgatgcg ctttgccgcc cgcagcaagg ttgcgccgac
      121 taccgaactg ttcccgatgt cgaaaattaa cgacgccatt cagcatgtac gcgatggtaa
      181 agcgcgctac cgcgtggtgt tgaaagcaga tttttgaaaa tcattcgcag cgctgatctg
      241 aggcgctgcc ctctttcgca catattctgt tttgtcttat cgccagcaca gcctgccgac
      301 attgttcggt gacattggca atatcatggt tgatatcaat gcgcacaata tcctgttcat
      361 ctgcttgtgg tcgctccagc gcctcaaact gactttttag taacgctacc ggcataaaat
      421 gcccagcccg acgctgcatt cgcgcgagaa tagtttcata atcgccatct aaccagagga
      481 aatgaacatg ggggctaccc ttgcgtaaaa tatcacgata ctgttttttt aatgatgaac
      541 agacaataaa tcctgtttca ttctttttat aaagactgta tgaagcatca tttaagcgtt
      601 ccagccaggg aagtcgatct tcatcagata atggaatgcc ttgcgacatt ttatctatat
      661 ttttggctgg atgaagatcg tcaccatcaa taaatttcgc agacaataac gcggcaacct
      721 tgctaccaat taatgtttta ccactccctg atacgcccat caaaataaag ctttcacccg
      781 ccatttaaaa tctcctgaat catcttacct gcggcgatac tacgcctggc ggcataataa
      841 tttcacgttt ttttatttct gttgataaga attacaaggc acatcacgtt atgcgtaaca
      901 tagtaatgta acaattttct gacgtgatct tcatcacaaa taatgacaat taaaaccgct
      961 taaatgcttc cgaggtgtct acctgaccag tgaggaattt atgcaagtga aaacacagtc
     1021 ctgcgttgtt gcgggcaaga aaactgttgc cgttaccgag cagacgatag attggaataa
     1081 taatggaaca ttagtacaaa taacccgagg tggaatttgc ggttccgatt tacattatta
     1141 tcaggaagga aaagtaggta atttcatgat aaaggcaccg atggtgttag gtcatgaagt
     1201 tatcggtaaa gttattcata gcgactcatc agaattacat gaagggcaaa cggtagccat
     1261 taatccgtct aaaccgtgcg gtcactgcaa atactgtatt gaacataacg agaatcagtg
     1321 tacagagatg cgtttttttg gcagtgccat gtatttccct catgttgatg gtggctttac
     1381 ccgttataaa atggtcgaaa cgtcgcaatg tgtcccttat ccggccaaag ctgacgaaaa
     1441 ggttatggct tttgccgaac ctttagccgt cgcgattcat gccgcacatc aggccggcga
     1501 gttacagggc aagcgagtat ttatttccgg tgttgggccc attggctgcc tgattgtcag
     1561 tgctgtgaaa acactggggg cctcggaaat tgtctgtgcc gatgtgagtc cccgttccct
     1621 tttgctggcc aaagagatgg gggcggatgt gctcgtaaac ccacaaaacg acgacatgga
     1681 tcactggaaa gcggaaaaag gctatttcga tgtcagcttt gaagtgtccg gtcatccttc
     1741 atcagtgaat acctgtctgg aggtcactcg tgcacgcggc gtaatggtgc aggtaggtat
     1801 gggaggcgcg atggcagaat tcccaatgat gacgttgatt ggtaaggaga tttcactcaa
     1861 aggctctttc cgttttacca gcgaatttaa taccgcagtg tcatggctgg cgaatggcgt
     1921 tatcaatcca ctgcctttac tgagtgctga atatcccttc actgacctgg aagaggcgct
     1981 acgtttcgcc ggtgataaaa cccaggcagc aaaagtccag cttgttttct aggagataaa
     2041 taaaggaata atacaatgaa cgatctattt tcactggcag gaaaaaatat cttgattacc
     2101 ggttcagcac agggcattgg ctttttactg gcaaccggcc tgggtaaata tggcgcacaa
     2161 ataattatta atgatattac tgccgaacgc gcagaacttg ctgtagaaaa actccgccag
     2221 gagggtattc gggccgttgc cgcacctttt aatgttaccc ataaacatga aattgatacc
     2281 gccgttgaac atatcgaaaa ggacatcggc cccattgatg tgctggtgaa taacgccggt
     2341 atccagcgcc gtcatccttt tactgagttc cctgaacaag agtggaatga tgtgatcgca
     2401 gtaaaccaga ccgccgtgtt cctggtgtcg caagcggtaa ctcgtcacat ggttgaacgc
     2461 aaggcgggta aagttattaa tatttgctcg atgcaaagcg aactgggacg tgacaccatc
     2521 accccttatg ccgcatcgaa aggagcggta aaaatgctca cccgcggcat gtgcgtcgag
     2581 ctggcgcgcc acaatattca ggtcaacggt attgcgccgg gctatttcaa aacagaaatg
     2641 actaaagcac tggttgagga cgaagccttc accgcctggt tgtgcaaacg gacccccgcc
     2701 gcacgctggg gcgatccgca ggaactgatt ggtgctgcgg tgttcctttc ttcaaaagcc
     2761 tctgatttcg taaacggcca cctgttgttt gttgatggcg gcatgttggt ggctgtttaa
     2821 cagccactac cctgcgtgca gcgcgaaagc gctgcttttc tggcactaat aagagataaa
     2881 gactatgcca ttaatcatta ttgcggcagg cgtcgcgctg cttcttatcc tgatgatcgg
     2941 ctttaaagtt aacggcttta ttgccctcgt tctggtagct gccgtcgtcg gatttgccga
     3001 agggatggat gcacaggccg tcctgcactc tatacaaaat ggtatcggca gcacgctcgg
     3061 cgggctggca atgatcctcg gtttcggggc catgttaggc aagctgattt ctgatacggg
     3121 cgcggcacaa cgtatcgcca ctacgctgat tgctactttt ggtaaaaaac gcgtgcaatg
     3181 ggcgctagtg atcaccggtc tggttgtggg cctcgccatg ttttttgaag tgggttttgt
     3241 cctgctgttg ccgttggtat ttaccatcgt agcatcatca ggattacccc tgttgtatgt
     3301 tggcgtacca atggtagcag cgctctctgt aacccactgt tttctgctgc cacatcctgg
     3361 tcctactgcc atcgcgacta tctttgaggc taatctcgga acgactctac tgtatggatt
     3421 tatcattacc attccgacag ttattgtcgc aggaccgctg ttttctaaac tgctaactcg
     3481 ctttgagaaa gcaccaccag aaggcttatt taatcctcat ctgtttagcg aagaggagat
     3541 gccctccttc tggaacagta ttttcgctgc cgtgatcccg gtcatcctga tggctatcgc
     3601 cgccgtttgt gaaattacgt taccgaaaac taacaccgtg cgcctcttct ttgaatttgt
     3661 cggtaaccct gccgttgcgc tgtttattgc cattgttatt gcgattttca cactgggccg
     3721 acgtaatgga cgcaccatcg agcaaatcat ggatatcatt ggggattcta taggcgctat
     3781 cgcgatgatt gtgtttatta tcgctggcgg cggcgcgttt aagcaggtat tagtagatag
     3841 cggtgtcggg cagtatattt cacacttaat gaccggaact acgctttcgc cgttattgat
     3901 gtgctggact gttgcggcgc tgttgcgtat cgctctgggc tctgccaccg tcgcggccat
     3961 taccaccgcg ggtgtggtgt tgccgattat caacgttacc catgccgatc ccgctttaat
     4021 ggtactggca accggtgcgg gcagcgtgat cgcgtcacac gtaaacgacc ctggcttctg
     4081 gctatttaaa gggtatttta atctgacggt tggtgaaacg ttgcgtacct ggacggtgat
     4141 ggaaaccctt atttctatta tgggtttgct gggcgtgtta gccattaacg ccgtattgca
     4201 ctgaaagaat aaaatattgt gatgtagttt cgggggaaga ttgaagattc ccccgacgtt
     4261 gggagaagtt atgcgcaatc acagaatttc tttacaggat atcgctacgc tggctggcgt
     4321 aacaaaaatg accgtgagtc gttatatccg ctcgccgaaa aaggtggcaa aggaaacagg
     4381 cgagcgcatc gccaaaatta tggaagagat caattatatc cctaatcgcg ctcccggcat
     4441 gttgttgaat gcacaaagtt ataccctcgg tatcttgatt ccgtcatttc aaaatcaact
     4501 cttcgctgac attctcgcgg gaatagaatc cgtcacctct gagcataatt atcagaccct
     4561 tattgctaat tacaattacg atcgcgattc cgaagaagag tcggtgatta acctgctctc
     4621 ttataacatt gacgggatta ttctttcgga aaaatatcac accatcagga cggtgaaatt
     4681 tctgcgttca gccaccattc cggtcgtgga attgatggat gtacagggag aacggctgga
     4741 tatggaggtc ggttttgata atcggcaggc cgcttttgac atggtgtgta ccatgctgga
     4801 taagcgagtc aggcgcaaaa ttctgtatct cggttccaaa gatgacaccc gcgatgaaca
     4861 gcgttatcag gggtattgcg atgcgatgat gctgcataat ctttccccgt tacgcatgaa
     4921 tccacgcgcc atctcatcca ttcatttagg aatgcaatta atgcgtgatg cccttagcgc
     4981 aaccccggat ttagacggtg tattttgcac caatgacgat attgcgatgg gtgcgcttct
     5041 gctgtgccgc gagcgaaacc tcgccgtgcc agaacaaatt tccattgcgg gttttcatgg
     5101 gctggagatt ggcaggcaaa tgatcccaag cctcgccagc gtaattactc cccgttttga
     5161 tattggtcgg atggccgccc aaatgttgct gagcaaaatt aaaaataacg atcacaacca
     5221 caacactgtt gacctcgggt atcagattta tcacggtaac acgctttagg ttattttctc
     5281 ttcattacac gtattctctt tgccggacgt tgcctatact ccaggcagga aactggagga
     5341 aatctcatga gcgaacccct gttaattgcc cgcacgccgg acacagaact gtttttactg
     5401 ccgggaatgg ctaaccgtca cgggctgatt actggcgcaa cggggacggg taaaaccgtt
     5461 acgctgcaaa aactggcaga gtcattgtcg gaaatcggtg ttccggtgtt tatggccgat
     5521 gtgaaaggtg atttaacggg agtagcggag gaaggtacat cttcggaaaa actgctcgca
     5581 aggcttaaaa atatcggcgt caatgactgg caaccacatg ctaatccggt ggtggtgtgg
     5641 gatatctttg gcgagaaagg ccacccggtg cgggcgacgg tttcagacct ggggccgctg
     5701 ttgctggcgc gactgttgaa tctcaacgat gtgcaatctg gcgtgctgaa tattatcttc
     5761 cgcattgcgg acgatcaggg attgttgctg ctcgacttta aagatttgcg ggcgattacc
     5821 cagtacatcg gcgataacgc caaatccttc cagaatcagt acggtaatat cagtagcgca
     5881 tcggttggtg ccatccagcg cgggctgtta tcgctggaac agcaaggcgc agcacacttc
     5941 tttggtgagc cgatgctgga tatcaaagac tggatgcgta gcgataccaa cggtaaaggc
     6001 gttatcaata tcctcagcgc cgagaagctt tatcagatgc cgaaactgta tgcagccagc
     6061 ctgctgtgga tgctttcaga gttgtatgaa caattgccgg aagcaggcga tctggaaaaa
     6121 ccaaaactgg tgtttttctt cgacgaagcg catctgctat ttaacgatgc accgcaggta
     6181 ctgctggata agattgagca ggtgataagg cttattcgct caaaaggcgt gggcgtctgg
     6241 ttcgtttcgc aaaacccgtc agatattccg gataatgtgc tcggacagct cggtaatcgc
     6301 gttcaacacg ctttgcgggc ttttacgccc aaagatcaga aagcagtaaa agctgcggcg
     6361 caaaccatgc gggccaatcc ggcgtttgat accgaaaagg cgattcagga actggggacc
     6421 ggcgaggcgt taatctcgtt tctcgatgcg aaaggaagtc cttcagtggt ggaacgggcg
     6481 atggtgattg ccccctgttc acgaatgggg ccggtgacgg aagatgagcg taacggcctg
     6541 attaatcact ccccggtgta tggcaagtac gaggatgacg tggaccggga atccgcctat
     6601 gagatgttgc aaaaaggctt tcaggccagt accgagcagc aaaataatcc tgccgtgaaa
     6661 gggaaagagg tggcggtgga tgacggtatt cttggtggat tgaaggatat tttgtttggc
     6721 actaccggac cacgcggcgg gaagaaagat ggtgtggtgc aaacaatggc gaaaagcgcc
     6781 gcccgccagg tgacgaatca gattgtgcgt ggtgtgctgg ggagtttgct gggggggaga
     6841 agaaggtaaa tcaggtgcga tttgtctttg cctgatgcga cgctggcgcg ccttatcatg
     6901 cctacactac tgcaatatat tgaattttca tgatttttca ggcatgaacg aagcacactt
     6961 tgctaacaat aataaaggga gctttcgctc cctttattcg ttcatcaggt tacgattttc
     7021 tcattaacag ccacaggctg attaagaaga agctggcgct tggcagcaac gcgccgataa
     7081 tcggcgggat gccgtaaacc aacgtcagcg ggccgaagat ctggtccagt acgtagaaga
     7141 caaaaccgaa gctgataccg gtgaccacac gcacgcccat cggtacgcta cgcagtgggc
     7201 caaagataaa cgacagcgcc atcagcatca tcaccgccac ggatagcggc tggaagattt
     7261 tgctccacat gttgagctga taacgtccgg catcctgacc gctcgacttc agatacttca
     7321 catagttgtg caaaccactg atggagaggg catccgggtc cagtgccacc acgcccagtt
     7381 tgtccggcgt gaggttggtt ttccaggtac cgctcaccgt ctgtgaacca gtgatctgtt
     7441 tcggattggt cagatcagat tcatcaacct gcgacagacg ccatacttta tgttccgggt
     7501 caaacttcgc ggtagcggca tagcgtacag attgcagacg acgattctca ttaaaggcat
     7561 agatactgat gccacctaac tcttcgtcac ctttaacccg ctcaatgtag acgaagttgt
     7621 tgccatcttt tgcccataaa ccttgctggg tagagagcaa cgagccaccg tacatcgcct
     7681 gcgcgcggta gttacgcgcc atctgctcgc cctgcggtgc gacccattcg ccaatcgcca
     7741 tcgtcaacaa gaccagcgga atggcggttt tcatcaccga cagcgccacc tgcatacggg
     7801 taaaaccaga agcctgcatg accaccagtt cgctgcgctg cgccaacatc ccaagaccaa
     7861 gcaacgcccc aagcagagcc gccatcggga agaagatttg cacatctttc ggcacgctca
     7921 gcaaggtata cattcctgcg cctaacgcgt cgtaactccc ctgcccggct tttttcagct
     7981 gatcgacaaa cttgataatg cccgacagcg acaccagcat gaacagcgtc atcatgatgg
     8041 tggtgaaaat agttttaccg atatagcggt caagtacgcc aaaaggttgc atcacaccgc
     8101 tcctttacgc gaaaaactgg cgcgcaggcg gcggaccggc acggtgtccc aaaggttgag
     8161 aacaatcgcc aaagccagat aaatcaggtt aacggtccac atccacagcg tcgggtccag
     8221 cttaccttta ccgccgttcg atttcaggga ggtctggatc aggaagaaaa gtagatacag
     8281 cagcatggct ggcagcatcg acagtacgcg cccctgacgc gggttaacca cgctcagcgg
     8341 aacgaccata agcgccatca taaacacggt gaataccagc gtgatacgcc agttcagttc
     8401 tgcgcgggca cgatcggtgt cagtgttcca caatgtgcgc atgtccatct ggtcggtatc
     8461 gttcgggtcg agcgccaccg cctggtgacc aatgatcgcc tgataatcct ggaagtccgt
     8521 aatgcggaaa tcgcgtaaca gtgcagtgcc ttcgaagcgc gttccctggt tgagagtgac
     8581 gacctgggag ccgtcgcgca gctgggttaa atgtccggaa tcggccacca ccacagaagg
     8641 acgtgcatta ccttttggtc gaatttgcgc gaggaacaca tctttgaaat cgctgccgtc
     8701 aacgctttcg atgaacagca ccgagctgcc attagtcgct tgctggaatt gcccttgcgc
     8761 cagcgctgcc atgccagggt tcgctttcgc ttctgctaac acttcatcct gatgtcgcga
     8821 tgaccacggt cccgcccaca tgacgttaac cgccgcgacg attgccgtga ataccgcaag
     8881 gatcattgcc gctttcacca acaccgcttt gctcaggccg caggcatgca ttaccgtaat
     8941 ttcactttcg gtatacagtt tgcccagcgt catcagcagc ccgaggaaca ggcttaatgg
     9001 caggataagc tgcgccattt ccggcacgcc caacccgagg agggagagca ccagattcgc
     9061 cggaatatcg ccatcaaccg ctgcgccgag gatcctcact aacttttgac agaaaaagat
     9121 caaaagcaag atgaagagta tcgccagctg gcttttgagc gtctcccgca ccagatatct
     9181 tatgattatc actttaaata cgcccgtaaa aactcgtctt ttgcaggatt ttagcttgtt
     9241 tcatggctta aacgtcattt attctcttga gtcgtcgaaa tcgtcgctaa gataattata
     9301 ctcaacggat tcacctctca gattttgttc tgacgtgcca atgccgtaat aacgttaaga
     9361 ttaacacgaa gtcatcgcaa cagcggacat gagttacgaa agcttgcaat tctatctgta
     9421 gccaccgccg ttgtctttaa gattcaggag cgtagtgcat ggagtttagt gtaaaaagcg
     9481 gtagcccgga gaaacagcgg agtgcctgca tcgtcgtggg cgtcttcgaa ccacgtcgcc
     9541 tttctccgat tgcagaacag ctcgataaaa tcagcgatgg gtacatcagc gccctgctgc
     9601 gtcggggcga actggaagga aaaccagggc agacactgtt gctgcaccat gttccaaatg
     9661 tactttccga gcgaattctc ctcatcggtt gcggtaaaga acgtgaactc gatgagcgcc
     9721 agtacaaaca agtcattcag aaaaccatta acacgctgaa tgatactggc tcaatggaag
     9781 cggtctgttt cctgaccgaa ctgcacgtta aaggccgtaa caactactgg aaagtgcgtc
     9841 aggctgtcga gacggcaaaa gagacgctct acagtttcga tcagctgaaa acgaacaaga
     9901 gcgagccgcg tcgtccgctg cgtaagatgg tgttcaacgt gccgacccgc cgtgaactga
     9961 ccagcggcga gcgcgcgatc cagcacggtc tggcgattgc cgccgggata aaagcagcga
    10021 aagatctcgg taatatgccg ccgaatatct gtaacgccgc ttatctcgcc tctcaggcgc
    10081 gccagctggc tgacagctac agcaagaatg tcatcacccg cgttatcggc gaacagcaga
    10141 tgaaagagct ggggatgcat tcctatctgg cggtcggtca gggttcgcaa aacgaatcgc
    10201 tgatgtcggt gattgagtac aaaggcaacg cgtcggaaga tgcacgccca atcgtgctgg
    10261 tcggtaaagg tttaaccttc gactccggcg gtatctccat caagccttca gaaggcatgg
    10321 atgagatgaa gtacgatatg tgcggtgcgg cggcggttta cggcgtgatg cgtatggtcg
    10381 cggagctgca actgccgatt aacgttatcg gcgtgctggc aggttgcgaa aacatgcctg
    10441 gcggccgagc ctatcgtccg ggcgatgtgt taaccaccat gtccggtcaa accgttgaag
    10501 tgctgaacac cgacgccgaa ggccgtctgg tactgtgcga cgtgttaact tacgttgagc
    10561 gttttgagcc ggaagcggtg attgacgtgg cgacgctgac cggtgcctgc gttatcgcgc
    10621 taggccatca cattaccggt ctgatggcga accacaatcc gctggcccat gagctgattg
    10681 ctgcatctga acaatccggt gaccgcgcat ggcgtttacc gctgggtgac gagtatcagg
    10741 aacagctgga gtccaatttt gccgatatgg cgaacattgg cggtcgtcct ggcggggcga
    10801 ttaccgcagg ttgcttcctg tcgcgcttta cccgtaagta caactgggca cacctggata
    10861 tcgccggtac cgcctggcgt tctggtaaag caaaaggcgc aaccggtcgc ccggtggcgt
    10921 tgctggcaca gttcctgctc aaccgcgccg ggtttaacgg cgaagagtaa ttgcatcagg
    10981 ctgtgttgtt attgtcggat gcggcgtgaa cgccttatcc gacctacaca gagcaccgga
    11041 cccgtaggcc tgataagacg cgacagcgtc gcatctggca atgttgttat tgtcggatgc
    11101 ggcgtgaacg ccttatccga ccgacacaga ataccggacc cgtaggcctg ataagacgcg
    11161 acagcgtcgc atcaggcaac gctgtgtatt accaaatgca tatttaaatc caccacaaga
    11221 agccccattt atgaaaaacg caacgttcta ccttctggac aatgacacca ccgtcgatgg
    11281 cttaagcgcc gttgagcaac tggtgtgtga aattgccgca gaacgttggc gcagcggtaa
    11341 gcgcgtgctc atcgcctgtg aagatgaaaa gcaggcttac cggctggatg aagccctgtg
    11401 ggcgcgtccg gcagaaagct ttgttccgca taatttagcg ggagaaggac cgcgcggcgg
    11461 tgcgccggtg gagatcgcct ggccgcaaaa acgcagcagc aacccgcgcg atatattgat
    11521 tagtctgcga acaagctttg cagattttgc caccgctttc acagaagtgg tagacttcgt
    11581 tccttatgaa gattctctga aacaactggc gcgcgaacgc tataaagcct accgcgtggc
    11641 tggtttcaac ctgaatacgg caacctggaa ataatggaaa agacatacaa cccacaagat
    11701 atcgaacagc cgctttacga gcactgggaa aagcagggct actttaagcc taatggcgat
    11761 gaaagccagg aaagtttctg catcatgatc ccgccgccga acgtcaccgg cagtttgcat
    11821 atgggtcacg ccttccagca aaccatcatg gataccatga tccgctatca gcgtatgcag
    11881 ggtaaaaata ccctgtggca ggtcggtact gaccacgccg ggatcgctac ccagatggtc
    11941 gttgagcgca agattgccgc agaagaaggt aaaacccgtc acgactacgg ccgcgaagct
    12001 ttcatcgaca aaatctggga atggaaagcg gaatccggcg gcaccattac ccgtcagatg
    12061 cgtcgtctcg gtaactccgt cgactgggag cgcgaacgct tcacgatgga cgaaggcctg
    12121 tccaatgcgg tgaaagaagt tttcgttcgt ctgtataaag aagacctgat ttaccgtggt
    12181 aaacgcctgg taaactggga cccgaaactg cgtaccgcaa tctctgacct ggaagtggaa
    12241 aaccgcgaat cgaaaggttc gatgtggcac atccgctatc cgctggctga tggcgcgaaa
    12301 accgcagacg gtaaagatta tctggtggtt gcgactaccc gtccggaaac cctgctgggc
    12361 gataccggcg tggccgttaa cccggaagat ccgcgttaca aagatctgat tggcaaatat
    12421 gtcattctgc cgctggttaa ccgtcgtatt ccgattgttg gcgacgaaca cgccgatatg
    12481 gaaaaaggca ccggctgtgt gaagatcact ccggcgcacg actttaacga ctatgaagtc
    12541 ggtaaacgtc acgccctgcc gatgatcaac atcctgacct ttgacggcga tatccgtgaa
    12601 agcgctcaag tgttcgatac taaaggtaac gaatctgacg tttattccag cgagatccca
    12661 gcagagttcc agaaactgga gcgttttgct gcgcgtaaag cggtcgttgc cgcggttgac
    12721 gcactcggcc tgctggaaga aattaaaccg cacgacctga ccgttcctta cggcgaccgt
    12781 ggcggcgtgg ttatcgaacc gatgctgacc gaccagtggt acgtacgtgc cgacgtgctg
    12841 gcgaaaccgg cggttgaagc ggttaagaac ggcgacattc agttcgtacc gaagcagtac
    12901 gaaaacatgt acttctcctg gatgcgcgat attcaggact ggtgtatctc tcgtcagctg
    12961 tggtggggtc accgcatccc ggcatggtat gacgacgcgg gtaacgttta tgttggccgc
    13021 aacgaagacg aagtgcgtaa agaaaataac ctcggtgctg atgttgccct gcgtcaggac
    13081 gaagatgttc tcgacacttg gttctcctcc gcactgtgga ccttctctac ccttggctgg
    13141 ccggaaaaca ccgacgccct gcgtcagttc cacccaacca gcgtgatggt atctggtttc
    13201 gacatcatct tcttctggat tgcccgcatg atcatgatga ccatgcactt catcaaagat
    13261 gaaaatggca aaccgcaggt gccgttccac accgtttaca tgaccggtct gattcgtgat
    13321 gacgaaggcc agaagatgtc caaatccaag ggtaacgtga ttgacccgct ggatatggtt
    13381 gacggtattt cgctgccgga actgctggaa aaacgtaccg gtaatatgat gcagccgcag
    13441 ctggcggaca aaatccgtaa gcgcaccgag aagcagttcc cgaacggcat tgagccgcac
    13501 ggtactgacg cgctgcgctt caccctggcg gcgctggctt ctaccggtcg tgacatcaac
    13561 tgggatatga agcgtctgga aggctaccgt aacttctgta acaagctgtg gaacgccagc
    13621 cgctttgtgc tgatgaacac agaaggtcag gattgcggct tcaacggcgg cgaaatgacg
    13681 ctgtcgctgg cggaccgctg gattctggcg gagttcaacc agaccatcaa agcgtaccgc
    13741 gaagcgctgg acagcttccg cttcgatatc gccgccggca ttctgtatga gttcacctgg
    13801 aaccagttct gtgactggta tctggagctg accaagccgg taatgaacgg tggaacagaa
    13861 gccgaactgc gcggtactcg ccatacgctg gtgactgtac tggaaggtct gctgcgcctc
    13921 gcgcatccga tcattccgtt catcaccgaa accatctggc agcgtgtgaa agtactttgc
    13981 ggcatcaccg ctgacaccat catgctgcag ccgttcccgc agtacgatgc gtctcaggtc
    14041 gatgaagccg cactggccga caccgagtgg ctgaagcagg cgatcgttgc ggtacgtaac
    14101 atccgtgcag aaatgaacat cgcgccgggt aaaccgctgg agctgctgct gcgtggttgc
    14161 agcgcggatg cagaacgtcg cgtaaatgaa aaccgtggct tcctgcaaac gctggcgcgt
    14221 ctggaaagta tcaccgtgct gcctgccgat gacaaaggtc cggtttccgt tacgaagatc
    14281 atcgacggtg cagagctgct gatcccgatg gctggcctca tcaacaaaga agatgagctg
    14341 gcgcgtctgg cgaaagaagt ggcgaagatt gaaggtgaaa tcagccgtat cgagaacaaa
    14401 ctggcgaacg aaggctttgt cgcccgcgca ccggaagcgg tcatcgcgaa agagcgtgag
    14461 aagctggaag gctatgcgga agcgaaagcg aaactgattg agcagcaggc tgttatcgcc
    14521 gcgctgtaat caacaccgtg atgaaaacga aggccggagc atgctccggc cttttttatc
    14581 tcttacagaa atggtaaaga aggcataact ccccgagaaa tactggctaa gaagccttta
    14641 tctggttgtt cttctttatt gattaaagga aggtcatcca aatcgcccag caaatacgta
    14701 ttttttgcct gccagtcaat catccatatt ttcaacagtg gataagccag accgcccgta
    14761 atcccggata tcaccaccaa cgcacacatg cgataaagca taccgtggta cgttaaagtt
    14821 gagcgaaaac gaatacgccc atcattcagt gacaggttgc tcataaaatg gtttcgcaac
    14881 gacaccgtta aataactggt gctaacagca atcccaaaat aatagattaa ctgcgcgatt
    14941 atcattttac gttgcatttc cataaattgc tgtaaattct caatgtcatc atttgcatat
    15001 acagaactat catacgcatt taatatttga tcgaagataa tataaccagc aacagcgaga
    15061 aaaggcaata aagctaaaaa tgcgagaatg gcatatttta tacaatacgt agtatctaat
    15121 ttcaccttga aacgatgtat accgaaactg gtattactcc agagaaaact cattaccaga
    15181 ctatataaag taccattaaa aataccaatg gaaacaatac caactattgc catcagaacc
    15241 atagatataa taacactact tgaactattg gcatgtagca tctttgtcga gataaagaaa
    15301 acagtcccca tcccaacggc cattaaaatc ggcaagaaaa aggtcaccca ccagaaccct
    15361 ttcatagagc aattaaaact aaatcttacg ccgttgagac tggtcattaa ggcctgatga
    15421 cgcagtcctt ttgctgccat aaatataagc aaaaccaaca gtaacaaagt caaaacacaa
    15481 ccaacaagcg gcatatctgc tgacactgtc attaagattg cgaaatagaa aaaaacaaaa
    15541 acaagacaac taacaaaaac attcccaccg gtaattccat aagaaaatcg ttgtccgtta
    15601 acttccatat tagcataaag ataacgctta catttcatta atgcccatgg taaatagata
    15661 cctaacgtta taatcgttaa caacacattc accacacaaa taagaaaata tctctcacct
    15721 gtaccatgaa aaacaaacga atgggacgga acatccattt cattaataac ttgagccata
    15781 aaaaatccct gaattaaaaa atccattgat ataacttata tatacagaca gtaaaatgtt
    15841 ctgcaacgtt acaatactct tatcagtaat aggctggcaa aaactttatt actttataag
    15901 tcgcttgcgc acgtaagaag agagtggtat taaacaaatg tataattact ttatttttat
    15961 tgggttaaac tatgaataac attgcgccgc aatcacctgt aatgcgtcgc ctgacgctgc
    16021 aggataatcc tgctatcgcc cgcgtcattc gtcaggtatc cgccgaatac ggtcttaccg
    16081 ctgataaagg ctacaccgtc gccgatccga atcttgacga gctgtatcaa gtgtatagtc
    16141 agcctggtca tgcgtattgg gtggttgagt acgatggtga agtggtcggc ggtggtggaa
    16201 ttgcgccatt gacaggtagt gaagcggaca tttgcgaact gcaaaagatg tattttcttc
    16261 ccgctattcg cggcaaaggg ctggcaaaaa aacttgcttt aatggcgatg gatcacgcac
    16321 acgagatggg cttcaaacgc tgttacctgg aaacaacggc ttttctgaca gaagcaattg
    16381 ctctgtatga gcatttaggc tttcaacata tcgactacgc acttggctgc acggggcatg
    16441 tcgactgtga agtacggatg ctacgtgatc tctaaaaaaa atgcccgaca gtgtgagcta
    16501 tcgggcattt caatccatta attagtggcg aaccccgtca tcgtcatcgt cgatgtactc
    16561 ttcatcgtcg ccatcttcac catttggatc ttcaaagtaa gtgccccaac cgtcatactc
    16621 gacgtcaaat ttctccgcca gcgtcatcag ttgttccacc tgagcatcaa tcaggtcggc
    16681 attcagggcg cattcactga ggatgtcgca gcaaatcacc acatcaccat cctctacttc
    16741 cagttcttcc ggatcggtga cttcatatcc gagtttaaac gcttctaccg ctgctttctc
    16801 cagcgtttca aggtcgtctg cggaaagatg gtgctcaatg gtgtacagcg catctggatc
    16861 gctgccatct tccagtaatt cttcaataat cagacgtgtc tcttcacgct gctcttccag
    16921 ttgttccggg tttgccatgg ctcattcctc ttaatgtcct gccgatactc ttattgtcac
    16981 acaccactga cattgcctct acctttgtga taaagattta tgctttagac ttgcaaatga
    17041 ataaccatac atataaattg aattttaatt catttaggcg ttagccacag gagggatcta
    17101 tgtccgggtt ttatcataag catttcctga aattactcga tttcacgcca gctgaactta
    17161 acagcctgct gcagttagcc gcgaagctga aagccgataa gaaaagcggt aaagaagaag
    17221 ccaaactcac tggtaaaaac atcgcgctca tcttcgaaaa agactcgacc cgtacccgat
    17281 gctctttcga agttgccgca tatgaccagg gtgctcgcgt tacttatctc ggtccaagcg
    17341 gcagccaaat tggtcataaa gagtcgatta aagacactgc ccgcgtgctt ggtcgcatgt
    17401 atgacggtat tcagtatcgc ggctatggtc aggagattgt cgaaacactg gcggaatacg
    17461 ctggcgtgcc ggtatggaac ggcctgacca atgagtttca ccccacgcag ctgctggcgg
    17521 atctcctcac catgcaggag catttgcccg gcaaagcgtt caacgaaatg acgctggtct
    17581 atgcaggtga cgcacgtaac aacatgggca attcgatgct cgaagctgca gcgcttaccg
    17641 gtctggattt gcgtctggtc gcgccacagg cgtgctggcc agaagctgcg ctggttacgg
    17701 aatgccgcgc cctggcacaa caaaatggcg ggaatattac gctgacggaa gatgtagcta
    17761 aaggagttga aggtgctgac tttatctata ccgatgtatg ggtgtcgatg ggtgaagcaa
    17821 aagaaaaatg ggctgaacgc attgcattac tgcgtgatta tcaggtgaac agcaagatga
    17881 tgcagttgac cggtaacccg gaggtcaaat tcctccactg cctgcccgcg tttcatgacg
    17941 accaaacgac gcttggcaaa aaaatggcgg aagagtttgg cctacatggc ggaatggaag
    18001 tgactgatga ggtcttcgaa tctgccgcca gcattgtatt tgatcaggcg gaaaaccgca
    18061 tgcataccat caaagcggtg atggtcgcga cgctcagtaa ataattgaca tctctataaa
    18121 gatgccggga aatacaaaaa atatcccggc atcatattat tttatgcagt gtgagttcta
    18181 ttcttccacc cccatttttc tattactgct cgtaaatttt ccggaggatt taatttttct
    18241 aactgttttt cattaatata attatatata ttgattttcc catcccggag gacttccatc
    18301 attcttgttc taatttttaa ttcattaacc atcttttctt caaaaatctt aacgacataa
    18361 cccggaagtt tatttacaat ttcttttgtt ttttcttcat caccataaac atatcctaac
    18421 tcattaagat acagctgttc gccctcataa cacttaagta attgatagaa gtaccactta
    18481 gcaaaatcag gttgcttcat gaaaaaaccc ttcaaaaaaa tatcatttac taaaatgctg
    18541 acaggcactt cattaaatag caacttaaca tcatcaaata actttagtat taagcgtgat
    18601 ttttcttgat tgcttaagtt aaaatcattg acatatgcaa ccaacttatc ccaaatcaat
    18661 ccctgtaatt tacagctatt tttatagggt tctttatcga gaagggaaaa taatgcctca
    18721 gctcttttag actgatcaca accagactta atatttttta tcacgtcgca tgcataatct
    18781 atctttgcag cagcattaaa atattcgata cttttattaa aacaccactc cagtattttt
    18841 acatcactca tatatggcga ttttaaaaaa gtttctaaca tcgaacggat gaccggtgag
    18901 aggtcaaccg gcgcatcatc taaggaatca ataagaatat gcgctagatc aattttgatc
    18961 ttattaattt catcattact ttttttatta ttagtaaagg catcgtcaga cgcgctgaga
    19021 ttatctatct cttcaagtat gccttcttcg cccagtaagt ctggaataat ttcattacat
    19081 acatgaggca ttattagcag atgctcttgc tttattgttg acaatgttgt agccaaggca
    19141 tctttaaaaa aatctttata gtcctgggct tgccaattca tctagttatt atttaactga
    19201 agtgctattt tgttgttaat ttcacgctgg taacacgcca gaactttatt agtaatcagt
    19261 agtaatgaac tctcatcttt tgtttcaaaa tccgtccctt caaaacgaaa aattgactta
    19321 ccagattctg ttacatcatc aaatataatg gctgtacgcc cttgacgagc cccattcaca
    19381 gagggagggg taagtctaac ttctaaaata accgggcgta tatcatcttc ccacccttca
    19441 ggattattca tgtaaaatcg cattatttca ctaccatcat ctttttttaa ataagatgag
    19501 gattttatat tacccatcat ttttcttccc gcttcattgc ggctttcact aaaatctaca
    19561 tgtcccaagg taaatatcca tgccagaaac tccaaaaatg ccttaggaga aaggatattt
    19621 cttttgttat gctgcccctc ggcaatacgg gaaaaattat tgattcccac acaccagcta
    19681 tttaaaacat catgttttgg tttaaaattc ctgcaggaag caacgtagtg aaaataatcc
    19741 gaatgcgata ttttgctcat gacgaactcc ttgagttaat aagaacgtct aagcattaca
    19801 aattttactc acaaaaaaat atcaaaaaac gctcttttca caaactcgcc gtaaacctgg
    19861 agttatttac ggcgaaaagt catcacgcca tcagcatctt cactactgct ttgcgaacct
    19921 gggctggcgc gcccactgcg cacagcggtt tatgcacttc ccccggataa aacacaacaa
    19981 aatcaccttc attaaggata actgttttct catcaacgcc ttccggcaaa aatgcgatgt
    20041 ctttgtctgc taaccagtcg gtatccgggg tgcctgcagg ttgtgtactg aaggtcatgc
    20101 cttcctgacc ttttaacaca atctgaatgt cgagatagcg ggcatggtac tccgcacggc
    20161 gagcttcgta cggctcggtc atatcttccg agataagata aaacagacga ttgccttcga
    20221 tatcgtgctt gcccttgggc gtttctgccg taacgtttgc tttgatatgc tcaatcgcct
    20281 ggcgtaactc ctggggtagc cacgactgaa gattatgaat atttccgatg atcatctttt
    20341 ccactcctta attaaaacaa cgtttcattt ctatcgttat acgaaaagat gatcaattcc
    20401 gctagcctta acgcgtgaat tattgtctta tcacaactta tcggcaggcg cgttcccgcc
    20461 tttcgggtca aggcccgcat cactccttga gaatagtttt tatcgctccc cccgccagtg
    20521 cagctgttgc acagagttgt tcgagggagt gcccctctcg tgccttcgca gaaagccctg
    20581 acatcacggt actcataaaa tcagtcacac attgtgcgct ttgtggatgc cgcctggcga
    20641 tatagtcata aatggccgtt tctgcggcgt gataatattg aacggcaata tcacgcgctt
    20701 gtggatcatg actatgaatg ccttcaagaa ccatacagcc agcgcagccg ccgttttggc
    20761 tatatctgcg cgccgcttct tttaatacct cagccaggca ctcccctacc ggacgatcat
    20821 cacgaagaat atcgacaagc ggaatagctt ccgtaccgac gtattcattg agtacacggc
    20881 taaataaccc agctttactg ccaaaagccg cgtagaggct cggggggtta ataccaagat
    20941 aatcagtaac ttcagcaaca ctgacagcat cgaaaccttt ttggtgaaaa agcacttttg
    21001 ccgcagagat tgcctgctca ggagcgaaac gtctgggacg acctggaacg cgagattgtt
    21061 ttttagtgac cattacaaaa cctgttgaca gaaagttaaa acagttttga aatgcatgtt
    21121 acataataaa tcaaggagtc cttatgggcg cttttacagg taagacagtt ctcatcctcg
    21181 gtggcagtcg tggtatcggt gccgctatcg tacgtcgttt cgtcaccgat ggggccaatg
    21241 tacgattcac ctatgcgggg tcgaaagatg ccgctaaacg cctggcacaa gagactggag
    21301 cgacagcagt attcacagat agtgctgaca gagacgctgt cattgatgtc gttcgtaaga
    21361 gcggcgcatt ggatatcctg gtggtaaatg caggtattgg cgtctttggc gaggccctgg
    21421 aattaaatgc cgacgatatt gatcgccttt tcaaaatcaa tattcatgct ccttatcatg
    21481 cctctgttga agccgcccgg cagatgcccg aaggcgggcg catcttaatc atcggctccg
    21541 tgaatggcga tcgtatgcct gttgcaggca tggcgcgcgg gctggcccgt gattttggac
    21601 cgcgtgggat caccattaac gtcgtccagc cagggccaat tgataccgac gctaatcccg
    21661 ccaacgggcc aatgcgcgat atgttgcata gtttgatggc tatcaaaaga catgggcaac
    21721 cggaagaggt cgctggtatg gtcgcatggt tagcagggcc agaagccagt tttgttaccg
    21781 gcgcgatgca taccattgat ggcgcgtttg gcgcataacc gactacgctc aattaagccc
    21841 agccattatc catgatgtct gggttttgtt tactcacgtc gtccgctaaa agcggctccc
    21901 ggtaaagaga aatcttctgt tcatgtcgta aatcttgagg ataaccaaat ggtagaaaga
    21961 accgctgttt tccctgctgg ccgacattca ctatatgctg agcatcgtta ttctgcggct
    22021 attcgttccg gcgatttgct gtttgtttcc gggcaagtag gaagtcgaga ggacggaaca
    22081 ccagaacccg attttcagca acaagtcaga ctggcatttg ataatttgca tgcgaccctg
    22141 gcagctgcgg gatgcacttt tgacgatatc attgatgtta cgagcttcca taccgatcca
    22201 gaaaaccaat ttgaagacat catgacggtg aaaaatgaaa tatttagcgc cccaccttat
    22261 ccaaactgga cggcggtggg tgttacatgg ctggcaggct ttgattttga aattaaagtg
    22321 atagcgcgca tccctgagca gtaagcaata gtgttagccg ttcgctttca cactccgccc
    22381 tataagtcgg atgaatggaa taaaatgcat atctgattgc gtgaaagtga aaaaggaaaa
    22441 agcagggaat gtctgcaatt attgataccg aaggacagtt cccctgcaga atcacatcaa
    22501 ataaaaatgc atataccttg acttttaatt caaataaacc gtttgcgctg acaaaatatt
    22561 gcatcaaatg cttgcgccgc ttctgacgat gagtataatg ccggacaatt tgccgggagg
    22621 atgtatggtt cagtgtgttc gacattttgt cttaccgcgt ctgaaaaaag acgctggcct
    22681 gccgtttttc ttcccgttga tcacccattc ccagcccctc aatcgagggg cttttttttg
    22741 cccaggcgtc aggagataaa agatggctaa tccgctatat cagaaacata tcatttccat
    22801 aaacgacctt agtcgcgatg accttaatct ggtgctggcg acagcggcga aactgaaagc
    22861 aaacccgcaa ccagagctgt tgaagcacaa agtcattgcc agctgtttct tcgaagcctc
    22921 tacccgtacc cgcctctctt tcgaaacatc tatgcaccgc ctgggtgcca gcgtggtggg
    22981 cttctccgac agcgccaata catcactggg taaaaagggc gaaacgctgg ccgataccat
    23041 ttcggttatc agcacttacg tcgatgcgat agtgatgcgt catccgcagg aaggtgcggc
    23101 gcgcctggcc accgagtttt ccggcaatgt accggtactg aatgccggtg atggctccaa
    23161 ccaacatccg acgcaaacct tgctggactt attcactatt caggaaaccc aggggcgtct
    23221 ggacaatctc cacgtcgcaa tggttggtga cctgaaatat ggccgcaccg ttcactccct
    23281 gactcaggcg ttagcgaagt tcgacggcaa ccgtttttac ttcatcgcgc cggacgcgct
    23341 ggcaatgccg caatacattc tggatatgct cgatgaaaaa gggatcgcat ggagtctgca
    23401 cagctctatt gaagaagtga tggcggaagt agacatcctg tacatgaccc gcgtgcaaaa
    23461 agagcgtctg gacccgtccg agtacgccaa cgtgaaagcg cagtttgttc ttcgcgccag
    23521 cgatctccac aacgccaaag ccaatatgaa agtgctgcat ccgctgccgc gtgttgatga
    23581 gattgccacc gatgtggata aaacgccaca cgcctggtac ttccagcagg caggcaacgg
    23641 gattttcgct cgccaggcgt tactggcact ggttctgaat cgcgatctgg tactgtaagg
    23701 ggaaatagag atgacacacg ataataaatt gcaggttgaa gctattaaac gcggcacggt
    23761 aattgaccat atccccgccc agatcggttt taagctgttg agtctgttca agctgaccga
    23821 aacggatcag cggatcacca ttggtctgaa cctgccttct ggcgagatgg gccgcaaaga
    23881 tctgatcaaa atcgaaaata cctttttgag tgaagatcag gtagatcaac tggcattgta
    23941 tgcaccgcaa gccacggtta accgtatcga caactatgaa gtggtgggta aatcgcgccc
    24001 cagtctgccg gagcgcatcg acaacgtgct ggtctgcccg aacagcaact gtatcagcca
    24061 tgccgaaccg gtttcttcca gctttgccgt gcgaaaacgc gccaatgaca tcgcgctcaa
    24121 atgcaaatac tgtgaaaaag agttttccca taatgtggtg ctggccaatt aattgcggtt
    24181 ggtaataaaa gtctggctcc ctataatgag ccagactttt taccgctgta ataaaggaga
    24241 aatcatgagc aaaactatcg cgacggaaaa tgcaccggca gctatcggtc cttacgtaca
    24301 gggcgttgat ctgggcaata tgatcatcac ctccggtcag atcccggtaa atccgaaaac
    24361 gggtgaagta ccggcagacg tcgctgcaca ggcacgtcag tcgctggata acgtaaaagc
    24421 gattgtcgaa gccgctggcc tgaaagtggg cgatatcgtt aaaaccaccg tgtttgtgaa
    24481 agatctgaac gacttcgcaa ccgtaaacgc cacttacgaa gccttcttca ccgaacacaa
    24541 cgccaccttc ccggcacgtt cttgcgttga agttgcccgt ctgccgaaag acgtaaaaat
    24601 tgagatcgaa gcgatcgctg ttcgtcgcta atcttgatgg aaatccgggc tatcatgccc
    24661 ggattaagtc tgatgacaaa cgcaaaatcg cctgatgcgc tacgcttatc aggcctacgt
    24721 gattcctgca atttattgaa tttgttggcc ggataaggca tttacgccgc atccggcatg
    24781 aacaaagctt actttgtctg caatctgaat cggggctatc gtgcccagtt tattctttat
    24841 tgccagccgt aacgacggct atagaaccct ttcaccaact gggttaatgt catataccct
    24901 gccagaatcg caaccagcca cgggaaatag cttaacggca gcgcctgtaa ttgcagataa
    24961 ctggccagcg gtgaaaacgg caatgcgatc ccgacaatca tcacgatcac ggtcatgatc
    25021 attaacggcc acgatgcaca gctctgaata aacggcacac ggcgggtacg gatcatatgc
    25081 acaatcagcg tttgcgacag taagcccacc acaaaccatc ccgactggaa cagcgtttgc
    25141 gtttccggcg tgttggcatg gaatacccac cacatcaggc aaaacgtcag aatatcgaag
    25201 atcgagctga tcggtccgaa gaagatcata aagcgcccca gatccgccgg gttccaacgc
    25261 tgcggctttt gaatttgctc gtcgtcgacg ttatcaaacg ggatcgccac ctgtgacaca
    25321 tcatacagca ggttctgaat aagcaagtgt aacggcaaca tcggcaggaa gggcaagaaa
    25381 gcactcgcca ccagcacgct gaacacatta ccgaagttgg agctcgccgt cattttgatg
    25441 tatttcagca tgttggcgaa agtgcgacgt ccttcaataa ccccctcttc cagcaccatc
    25501 aggctttttt ccagcaggat gatatcagcc gcttcacggg caatatctac cgcgccgtcc
    25561 acagaaatgc cgatatccgc agcgcgtaag gcgggcgcat cattaatacc atcgcccata
    25621 aagccaacca catgcccttc gcgcttcagt aaggtcacaa tgcgttcttt atgcatcggc
    25681 gtcagacgag caaacagcgt ggtgcgctgt gcgagatttg ccagttcgtc gtcagatagc
    25741 gtttcaatat cactaccaat gaccacctct cccgcgtcca gccccacttc atggcacact
    25801 ttcgctgcaa ctaactcact gtcgccagta aggattttta cggtaattcc actcgctttt
    25861 aatgccttca gtgccggagc agttgtctct tttggcggat caagaaaagc aatatatcct
    25921 tcgaggatca ggtcggattc atccgcccgc tggtaatctc cttcacgcgc tggcaggtat
    25981 ttcgtcgcca ccgcaaccac gcgcagcccc tgacgattca gcgtatcagt aacccgctta
    26041 atcttacgca gcatgatgtc atcgagcggc acaatctcgc cattgtgacg cacctgcgaa
    26101 cacacattga ggatttcctg caatgcacct ttgcaaacca gctgatggtg ctcggtattt
    26161 tctgccacta ccaccgacat ccggcgacgc tcgaaatcaa acggaatctc atcgattttt
    26221 tgccaacgac tggccagcga gcgcgctgac tcttcatccg taccttcgag caccgctgta
    26281 tcgagcaggt ttttaagtcc ggtctgataa tgactgttca accacgcgct atgcagcacg
    26341 cgttcgctgg ttttaccgga gatatcggta tgattctcca gcacaatttt gtcctgtgtc
    26401 agggtgccag ttttatcagt gcacagaata tccattgcgc caaagttctg aatagcatcc
    26461 agatgtttga cgatcacttt ctgtttcgac agctttactg cgccgcgcgc cagcgtcgag
    26521 gtaacaatca tcggcaacat ttccggcgtt aagcctaccg cgaccgaaag cgcaaacagt
    26581 gccgcttccc accagtcgcc tttggtgtaa ccattgatta acagcaccac cggagccatc
    26641 accagcataa agcgaatcag cagcatactg acgcggctga tcccttgctg aaaggcattc
    26701 ggctcgcttt cctgctcact aacacgcccc gccagttgac caaaccaggt attggcacct
    26761 gtagcaatca ccattgcttg tgccgtgccg ctcaccacgg tggtgcccat aaaacacagg
    26821 gtgtcgcact ccagcggatt gctgtgctcc ggctggcgag tggtagctgc tttttctacg
    26881 ggcagagact caccggttaa cgacgcctga gcgacgaaca gatcccgcgc ctgcaagata
    26941 cgtaaatctg ccgggatcat atcccccgcc gccagtttta taatatcgcc gggcaccagc
    27001 tggtcgatcg ggatctccag ccagccattt tcgcctttgt cgttaattac gcgcagcacc
    27061 gtcgcagtat tgctgaccat cgctttcagg gcatctgccg ctttagtgga acgtgcttcc
    27121 tgaataaagt tcagcaacgt agaaatagcg accattagcg cgataacgcc cgcggcaaat
    27181 aaatcttccg tggcgtaaga aatagcgccg agaatggtga gtaaaatatt aaatgggttg
    27241 cgatagcaga cccataaatg tacccaccac ggcgacggtt gttgtgcggg taatttattt
    27301 tcaccatgtt gttcgcgggc agattccact tccgcctgat ttaacccttc cggatgggtg
    27361 tcgaacgttt tccataattc ttcttcgggc atcaccgcca ttttcaggca atgcgcactt
    27421 aaggacggcg ggaccgccgt attcactgtc tgttgcgcgc caggcaatgg atcgcgatga
    27481 accagacggg aaggtaaatg gcgaatgagc cgggtaaaaa tttctttaaa cataaggagt
    27541 ccctccgcac tgtctgaaac agtacagaaa tgtcttcagg cgtggcgaag ccttacctga
    27601 aggtaataaa gctgagcagg gacgttaaaa cgtcacggcc ttacgcatcc ggtaacgccg
    27661 tgaaaagacg ggcttaccgg aaaagtttta atctccgtcg agggagaggc gtgggatcag
    27721 gttccatata acctccggta agtgaaaaaa gcgccgcaaa taataaggag aatccaataa
    27781 tattcgtagc atctatggcg atataatacc tgctggcaac taaaccaacg ataaaccaga
    27841 ctttacccat tgctgaatgc acgggtaaag ttaggctcaa ataattaaac aacacgttac
    27901 aggacaacag gatgcaaaat cggctgacca tcaaagacat cgcacgctta agcggcgtgg
    27961 ggaaatctac agtttcccgg gtgctgaata acgaaagcgg cgtgagccag cgcacccgcg
    28021 agcgtgttga agcagtgatg aatcagcatg gattttcccc ttcccgctct gcgcgcgcta
    28081 tgcgtgggca aagcgataaa gtggtcgcca tcattgttac ccgtctggat tcgttgtcag
    28141 aaaatctcgc cgttcaaacc atgctgccag cgttctatga acaaggttac gacccaatca
    28201 tgatggaaag tcagttttcc ccacaattag ttgccgaaca tttgggggtg ctgaaacggc
    28261 gtaatatcga cggcgtagtg ctgttcggtt ttactggcat aacagaagaa atgttagccc
    28321 actggcagtc atcgctggtt ctgctggcgc gtgacgcaaa aggctttgct tcggtctgtt
    28381 atgacgacga aggggcaatc aagatcctga tgcaacggct gtatgaccag ggacatcgta
    28441 atatcagtta tctcggcgtg ccgcatagtg acgtgacaac cggtaagcga cgtcacgaag
    28501 cctacctggc gttctgcaaa gcgcataaac tgcatcccgt tgccgctctg ccagggcttg
    28561 ctatgaagca aggctatgag aacgtggcaa aagtgattac gcctgaaact actgcgttac
    28621 tgtgcgcaac cgacacgctg gcacttggcg caagtaaata cctgcaagag caacgcatcg
    28681 acaccttgca actggcgagc gtcggtaata caccgttaat gaaattcctc catccggaga
    28741 tcgtcaccgt agatcccggt tacgccgaag ctggacgcca ggcggcctgc caattgatcg
    28801 cacaggtaac cgggcgcagc gaaccgcaac aaatcatcat ccccgccacc ctgtcctgat
    28861 cgtttcctga acgataaatt gtgatcttcg ctgcgttccg ggaacgttcc cgtttttaaa
    28921 tttttccgcg caatatattc tgcagccaac caaaaatgtc atctgccatg gggctttatg
    28981 atgagcaaaa taaaccaaac ggatatcgat cggttgattg aactggtcgg cgggcgcggc
    29041 aatattgcga cggtgagcca ctgtattact cgcctgcgct ttgtcctcaa ccaaccggcc
    29101 aatgccagac cgaaagaaat tgagcaactc cccatggtga aaggctgttt caccaatgcc
    29161 gggcaatttc aggtggtgat tggcaccaat gtgggtgatt actatcaagc actgattgcg
    29221 tcaaccggac aggcgcaggt tgataaagag caggtaaaaa aagctgcccg gcagaatatg
    29281 aaatggcatg agcagttgat ctctcatttc gcggagatct tcttcccgtt gctgcccgcg
    29341 ttgattagcg gcggtttgat cctcggtttt cgcaatgtga tcggcgattt gcccatgagc
    29401 aacggtcaaa cgctggcgca aatgtaccct tccctgcaaa cgatctacga ttttctgtgg
    29461 ttgatcggtg aagcgatctt cttctacctg ccggtcggga tttgctggtc agcggtgaaa
    29521 aaaatgggcg gcacgccgat cctcggtatc gtgcttggcg tgacactggt ttctccacag
    29581 ctgatgaacg cttatctgct cgggcagcag ctgccggaag tgtgggactt tggcatgttc
    29641 agcatcgcca aagtaggcta tcaggcgcag gtgatcccgg cactgttagc cggactggcg
    29701 ctgggcgtta ttgaaactcg ccttaaacgc atcgtgccgg attacctcta tctggtggtg
    29761 gtacccgtct gttcgctgat cctcacggtg ttcctcgccc atgcgctgat tggtccgttt
    29821 ggtcgcatga ttggcgatgg cgttgccttt gcggtacgtc acctgatgac cggcagcttt
    29881 gctccgattg gtgcggcatt gtttggcttc ctgtacgcgc cgctggtgat caccggcgta
    29941 caccagacca cccttgctat tgatttgcag atgattcaaa gcatgggtgg cacgccagtg
    30001 tggccgctga ttgcgctgtc gaatatcgct cagggctccg ccgtgatagg cattatcatt
    30061 tccagccgca agcacaatga acgcgagatc tccgtgcctg ccgctatctc cgcctggctt
    30121 ggggtcactg agcctgcaat gtacggcatc aacctgaaat atcacttccc gatgctgtgc
    30181 gcgatgattg gttctggtct ggcaggattg ctatgcggcc tgaacggcgt tatggcgaat
    30241 ggcatcggcg taggcggcct gccgggaatt ctctcgattc aaccgagcta ctggcaggta
    30301 tttgcgctgg caatggctat cgccatcatc atcccgattg tactcacctc gtttatctat
    30361 cagcggaaat accgcctggg cacgctggac attgtttaat tttcttcggg gcgcaattgc
    30421 gccctctcgc atttgcagga ataacgtaat gactaatctt ccccactggt ggcaaaacgg
    30481 cgttatctac cagatttatc caaagagttt tcaggacacc acgggtagcg gcaccggcga
    30541 tttacgtggc gttatccaac gcctggacta tctgcataaa ctgggcgttg atgccatctg
    30601 gctaaccccc ttttatgtct ctccccaggt cgataacggt tacgatgtag cgaactatac
    30661 ggcgattgat cccacctacg gcacgctgga cgattttgac gaactggtga cgcaggcaaa
    30721 atcgcgcggg attcgtatca ttctcgatat ggtgtttaac catacctcta cccaacatgc
    30781 ctggtttcgc gaggcgctga acaaagaaag cccttaccgc cagttttata tctggcgcga
    30841 tggcgaacca gaaacgccac cgaacaactg gcgttcaaaa tttggcggta gtgcgtggcg
    30901 ctggcatgcg gaaagcgaac agtactattt gcatctcttt gcaccagaac aggcggatct
    30961 caactgggag aatccagcgg tacgcgcaga gctgaaaaaa gtctgtgaat tctgggccga
    31021 tcgtggggtc gacgggttgc gcctggatgt ggtgaatctg atctccaaag acccgcgttt
    31081 ccctgaagac ctggatggcg acgggcgtcg cttctacacc gacgggccac gagcacacga
    31141 gtttttgcac gagatgaacc gcgatgtgtt tacgccacgc gggttaatga ccgtaggtga
    31201 aatgtcctcc accagccttg agcattgcca gcgatacgcg gcactgacag gcagtgaatt
    31261 gtcgatgacc tttaattttc atcacctgaa ggtcgattat cccggtggtg aaaaatggac
    31321 gctggctaaa cctgactttg tggcgttgaa aaccttgttc cgccactggc aacaaggaat
    31381 gcacaacgta gcatggaatg ccttgttctg gtgtaaccac gatcagccgc gcattgtttc
    31441 tcgctttggt gatgaaggtg aataccgcgt gcctgcggca aaaatgctgg cgatggtgct
    31501 gcatggcatg cagggaacgc cgtatatcta ccagggcgaa gagattggca tgaccaaccc
    31561 gcatttcacg cgcattactg actatcgcga cgtggagagc ctcaatatgt ttgccgagct
    31621 gcgcaacgat gggcgtgatg ccgacgagtt attggcaatc ctcgccagta aatcccgtga
    31681 caacagtcgc acgcccatgc aatggagcaa cggcgataat gccgggttta cggctggcga
    31741 accgtggatt ggcctgggcg ataactatca acaaatcaac gtagaagccg cgctgaccga
    31801 tgagtcctcg gtgttttaca cctaccaaaa gttaatcgca ctgcgtaagc aagaagccgt
    31861 tctgacatgg ggcaattacc aggatctgct gccaaacagc cctgtattgt ggtgctatcg
    31921 ccgtgaatgg aaggggcaaa ccttgctggt cattgccaac cttagccgtg ggatccaacc
    31981 ctggcagcca gggcaaatgc gcggcaactg gcagcttgtg atgcataact acgaagaagc
    32041 ctcaccacaa ccctgtgcca tgaatttacg gccttttgag gctgtctggt ggttacagaa
    32101 gtaaatcttc cctaagcccc ggtaatgccg gtcattccgg ggttttgtct gtcagcatgt
    32161 tgtttttgtt gatttttcaa ccagcaaatt cattaaaaaa tttacataac gctgtagcgc
    32221 ccgtcatccg tacgctctgc tttttacttt gagctacatc aaaaaaagtt caaacatcct
    32281 tgatgcaaag cactatatat agactttaaa atgcgtccca acccaatatg ttgtattaat
    32341 cgactataat tgctactaca gctccccacg aaaaaggtgc ggcgttgtgg ataagcggat
    32401 ggcgattgcg gaaagcaccg gaaaacgaaa cgaaaaaacc ggaaaacgcc tttcccaatt
    32461 tctgtggata acctgttctt aaaaatatgg agcgatcatg acaccgcatg tgatgaaacg
    32521 agacggctgc aaagtgccgt ttaaatcaga gcgcatcaaa gaagcgattc tgcgtgcagc
    32581 taaagcagcg gaagtcgatg atgccgatta ttgcgccact gttgctgcgg ttgtcagcga
    32641 gcagatgcag ggccgcaacc aggtggatat caacgagatc cagaccgcag ttgaaaacca
    32701 gctgatgtcg ggtccataca aacaactggc tcgtgcttac atcgagtacc gtcacgatcg
    32761 cgacattgaa cgtgaaaaac gcggtcgcct gaaccaggag atccgtggtc tggtcgagca
    32821 gaccaacgcc tcgttactca acgaaaacgc caacaaagac agcaaggtga ttccaaccca
    32881 gcgcgacctg ctggccggga tcgtggctaa acactatgca cgccagcacc tgctgccgcg
    32941 tgacgtggtg caggcacatg agcgtggcga tattcactat cacgatctcg attactcgcc
    33001 gttcttcccg atgttcaact gcatgttgat tgacctgaaa ggcatgctga cccagggctt
    33061 taaaatgggg aacgccgaga ttgaaccgcc gaagtcgatc tctacggcaa ccgctgtaac
    33121 tgcgcagatt attgctcagg tcgccagcca tatttatggc ggcaccacca ttaaccgtat
    33181 cgatgaagtg ctggcaccgt ttgtcaccgc cagctacaac aaacatcgca agaccgcaga
    33241 agagtggaac atcccggacg ccgaaggcta cgctaactct cgtaccatta aagagtgcta
    33301 cgatgccttc cagtcgctgg agtacgaagt aaacaccctg cacaccgcca acggtcagac
    33361 gccgtttgta acctttggtt ttggtctggg caccagctgg gaatcgcgcc tgattcagga
    33421 atctatcctg cgtaaccgta tcgcaggcct cggtaaaaac cgtaaaactg cggtgttccc
    33481 gaaactggtg tttgcgattc gcgatggtct gaaccataaa aaaggcgatc cgaactacga
    33541 catcaaacag ctggcgctgg agtgcgcaag caagcgcatg tatccggata tcctgaacta
    33601 cgatcaggta gtgaaagtca ccggttcgtt taaaaccccg atgggctgcc gcagcttcct
    33661 cggcgtgtgg gaaaatgaaa acggcgagca gatccacgat ggtcgtaaca acctcggcgt
    33721 gatcagcctg aacctgccgc gtattgctct ggaagcaaaa ggcgatgaag ccaccttctg
    33781 gaagctgctg gatgaacgtc tggtgctggc acgtaaggcg ctgatgaccc gtatcgctcg
    33841 tctcgaaggc gtgaaagcgc gcgtggcccc gatcctctat atggaaggtg cttgtggcgt
    33901 gcgtctcaat gctgacgatg atgtttctga aatcttcaaa aacggtcgtg cgtctatttc
    33961 gctgggttac atcggcatcc acgaaaccat taacgcgctg ttcggcggcg agcatgttta
    34021 cgacaacgag cagcttcgcg cgaaaggtat cgccattgtt gaacgtctgc gtcaggcagt
    34081 ggataagtgg aaagaagaaa cgggttatgg tttcagtctc tacagcacgc cgagtgaaaa
    34141 cctgtgcgac cgcttctgcc gtctcgatac tgctgagttt ggcgtggtgc cgggcgtgac
    34201 cgataaaggt tactacacca acagtttcca cctcgatgtg gagaagaagg tgaacccgta
    34261 cgacaagatc gactttgaag cgccttaccc gccgctggcg aacggtggtt tcatttgcta
    34321 cggcgagtat ccaaacattc agcacaacct gaaggcgctg gaagatgtct gggattacag
    34381 ctatcagcat gtaccgtatt acggcaccaa tacaccgatt gatgagtgct acgagtgtgg
    34441 ctttaccggt gagttcgagt gcaccagcaa aggcttcact tgcccgaaat gtggtaacca
    34501 tgacgcctcc cgtgtgtcgg tgactcgccg cgtgtgcgga tatttaggta gcccggatgc
    34561 acgtccattt aacgccggta agcaggaaga agttaagcgc cgcgttaaac atttggggaa
    34621 tgggcagata ggttaatctt cactctacgt taagcaaatg ccggatgcgg cgtaaacgcc
    34681 ttatccggcc tacatggcaa caacgattgt aggcctgata agacgcgaca gcgtcgcatc
    34741 aggcgttgat taccggatgc atcctatacg cggactgata agccaggcga aacaatgaat
    34801 tatcatcagt actatcctgt cgacatcgtc aacggccccg gcactcgctg caccctgttt
    34861 gtctccgggt gtgttcatga atgccccggt tgctataaca aaagcacctg gcgggtaaat
    34921 tccggtcagc catttaccaa agcaatggaa gaccagatca ttaacgatct gaatgacact
    34981 cgcatcaaac gccagggtat ttccctctcc ggcggcgatc cgctgcatcc gcaaaacgtg
    35041 ccggatattc tgaaactggt acaacgcatc cgcgccgagt gtccgggtaa agacatctgg
    35101 gtgtggacag gctataaact cgacgaactc aacgctgcgc aaatgcaggt tgttgatttg
    35161 attaacgtgc tggtcgacgg caaatttgtg caggatttaa aagacccttc cctgatctgg
    35221 cgcggcagca gcaatcaggt ggtgcatcat ttgcgatgat aagttccctg ttacctgagt
    35281 gcaggcaaca gggaaatgag gaattaacga tacttctggt gataggcgtt gcgggtcgtt
    35341 ttcagttgct ctgcggcagc ctgcgcttct tttactttac cttcattagc cagcttcagc
    35401 gcgtcgtcaa tctggccaac cagaatgtcg aaaccgtggc ggaagtcttt catttccggg
    35461 ctgtctggcg attttccttc cagcttcggc ggcgttgctt tttgcgcatc cagcgctgcg
    35521 gcgcgcatct tcgttaacgc gtctttgact tgcgccgcgt tatcggcttt ttcgaccact
    35581 tttaaattgt cgttgagggt ttccatattg tcttcaagat cagcggcaaa cgacgccgaa
    35641 ctgaatacca acgaggagac tgcaagaata gctaacaggc ttttacgcat tgctcacttc
    35701 cttttttatt atgaagattt ggcgcggcag gtcgcgccat ttttttactg cccggcgatt
    35761 ttcatctccg gcaacagcac agaaccacac tgtatattac tgcgtgtttc aatatcgtta
    35821 ccgacggtga caatattgcg ccacatatct tttaaattac ctgcgatggt gatttcgcta
    35881 accggatact gaatttcgcc gttttctacc cagaagcccg ccgcaccacg ggaataatca
    35941 ccggtaattg cactcacgcc ctggcccatc agctccgtca ccaccagccc ggtgcccatc
    36001 tctttgagca tttgttcgaa gcttagccct tgtccggcaa tccgccagtt gtgaataccg
    36061 cccgcatgtc cggtgctttt cagccccagt tttcgcgccg aatagctggt cagcagccac
    36121 tgagtcagga tgccatcttt aataatatcg cgacgctcgg tgcgcacacc ttcgctgtcg
    36181 aatggcgtcg acgccagccc tttcagcaga tgcggatgct cttcaatggt cagccagtcc
    36241 ggcagaattt gtttacccag cgaatccagc agaaaggtag atttacggta aaccgagcca
    36301 cccgctatcg cccccaccag atggccaaaa agcccggttg ccacttcatt ggcaaaaatg
    36361 accggcgctt tcatggtgga gagtttacgc ggtgacaggc gcgataaagt acggcgagca
    36421 cagtcggccc caacccactc tggcgtttgc agatcgctca tcgcacgacc aatggtgtag
    36481 gcgtaatcac gctccatatc gccattttct tcggcaatta cacagctgga gagcgaatga
    36541 cgcgttgagc agtaaccctg caacatgccg tggctgttgc caaaaacttt gacaccgtag
    36601 tggctgttaa agctgccacc ttcggtattg gtgatgcgtt tgtccgcctg caatgccgcc
    36661 tgttctgcgc gggccgccag ttcaatggct tcatccgggg aaacttccgc agggtggaac
    36721 aagtcgagat ctggtgcgtc aaaggccagc agctctttgt ctgccacgcc ggcacaggga
    36781 tctggcgagg tataacgggc aatatccagc gccgcctgta cagtacgggc aatggcctgc
    36841 gggcttaaat cggtggatga tgcgctacct ttgcggttct ggtgataaac agtgatcccc
    36901 agcgcgccat cgctattgaa ttcaacattc tccacttcac cataacgcgt gcttacgcta
    36961 atgccggtgg tcttgctgac ggcaacttcc gcaccgtccg atttgcctga ggccaactcc
    37021 agcgcagtgg aaactgcttc ttccagaatc ttgcgctgcg cttcaacttg agagattact
    37081 ttcattgcaa gtgccataat gtagagagtt tctctgaagt ctaacagaga accgtttttc
    37141 agtgcgcatc ttaactggta acattagcct ctttttttaa ggagcctgag atgactaagc
    37201 agcccgaaga ctggctcgac gacgttcccg gtgatgacat cgaagacgaa gacgatgaaa
    37261 ttatctgggt cagtaaaagt gaaattaaac gtgatgccga ggagctaaaa cgccttggcg
    37321 cggaaattgt tgatctgggg aaaaacgcgc tggataagat cccgttagat gcggatctgc
    37381 gcgcggctat tgagctcgcc cagcgtatta agatggaagg tcgccgccgc cagttgcagc
    37441 tcatcggtaa gatgctgcgc cagcgcgatg tagaaccgat ccgtcaggcg ctggataagc
    37501 tgaaaaaccg tcacaaccag caggtggtgc tgttccataa actggaaaac ctgcgcgatc
    37561 gtctgatcga tcaaggtgat gacgccatcg ctgaggtatt aaatctgtgg cccgatgccg
    37621 atcgtcagca gctgcgtact ctgatccgca acgcgaagaa agagaaagaa gggaataagc
    37681 cgccgaaatc cgcacgccag attttccagt atctgcgcga actggcggag aatgaagggt
    37741 aattttcgtt gttgatgtga gtatggtttg ccggatgcgg tgcacctgta ggcctgataa
    37801 gacgcatcaa gcgtcgcatc aggcactatg ctcaaatgcc ggatgcggcg tgaacgcctt
    37861 atccggccta cgcacactat atcaggctga ggccgaatta ctgcgcagct tccgccttct
    37921 tcgccagacc atccagcagt ttctgatgga tcccaccaaa accgccgttg ctcatcacca
    37981 gaatatggtc gccaggctga gcggttttca ccaccatatc tgccagcgta tccacatcgc
    38041 cactccagtg tgcaggctga acgcaggctt ctgccacttc tgccacctgc cacggaatat
    38101 gcgccggttg cagcaggaag acttcatcgg cacgacctaa tgaaggtgcc agatcgtctt
    38161 tgcagatccc cattttcatg gtattcgagc gcggttccag cacagcaata atgcgcgccg
    38221 taccaccaac tttgccacgc agcgccgcca gcgttgccag aatcgccgtc gggtgatggg
    38281 caaaatcgtc atataccgtg acgccattcg cttcaccacg caactccaga cggcgacgag
    38341 cattaataaa cgaacccagc gcgttagcgg catctgccgg cgctacacca acatggcgag
    38401 ccgctgcaat cgccatcagg ccattgtgca tattatgttc gcctaccagc gaccatttca
    38461 cttcgcccac tttttcgcca tccagcaaaa cttcccattc ggaagcatcg gtggtcagct
    38521 ttttcgcctg ccagtgaccc tgctcaccca ccagctcctg ctcgctccag cagcccatcg
    38581 ccatggtctg tttcaggttg atgtcatttt ccggccagat aatacggccc tgccccggaa
    38641 cgatacgcac cagatggtgg aactgtttct ggatcgcttt caggtcgtca aagatatcgg
    38701 cgtgatcgaa ctcaaggttg ttgaggatca gcgtacgcgg gcagtaatgg acaaatttag
    38761 agcgtttgtc gaagaaggcg cagtcgtact catccgcttc gataacaaag aagtcgcttt
    38821 cgcccagacg cgccgaaacc tcaaagttcc ccggcacacc gccgatcaca aatcccggtt
    38881 tgtaaccgca ctgttccaga atccaggtcg ccattcccgc ggtggtagtt ttgccgtgtg
    38941 taccggcaac ggccagcacc cagcggtcgc gcagcacaaa atcgtgcagc cactgtggac
    39001 ctgacatata agggatgttt ttttccagta ccgcttccac acacggattt ccacgggtca
    39061 tggcgttgcc aataatcacc agatccggct gcggatcgag ctggctggca tcgtaaccct
    39121 gaatcagctc aatgccttgc ttctcaagta aggtgctcat cggcggatac acattggcgt
    39181 ccgaacccgt tacttcatgg cctaactggc gcgccagcat cgccagaccg cccataaatg
    39241 tgccacaaat tcctaaaata tgaatgcgca tacgtcacta tcctttttta atctgccgct
    39301 cattttacgc atatgtcggg caagtgagaa acgcatttca ggataatccg caatttgctg
    39361 gcgcgattca cctgagcgca acattgagat tatttgttaa gattgttgcg gtcgctttac
    39421 tccataaaca ttgcagggaa agttttatga aaacgttagg tgaatttatt gtcgaaaagc
    39481 agcacgagtt ttctcatgct accggtgagc tcactgcttt gctgtcggca ataaaactgg
    39541 gcgccaagat tatccatcgc gatatcaaca aagcaggact ggttgatatc ctgggtgcca
    39601 gcggtgctga gaacgtgcag ggcgaggttc agcagaaact cgacttgttc gctaatgaaa
    39661 aactgaaagc cgcactgaaa gcacgcgata tcgttgcggg cattgcctct gaagaagaag
    39721 atgagattgt tgtctttgaa ggctgtgaac atgcaaaata cgtggtgctg atggacccac
    39781 tggatggctc gtccaacatc gatgttaacg tctctgtcgg taccattttc tccatctacc
    39841 gccgagttac gcccgtcggc acgccggtaa cggaagaaga tttcctccag cctggtaaca
    39901 aacaagttgc ggcaggttac gtggtatacg gctcctctac catgctggtt tacaccaccg
    39961 gatgcggtgt tcacgccttt acttacgatc cttcgctggg cgttttctgt ctgtgccagg
    40021 aacggatgcg cttcccggag aaaggcaaaa cctactccat caacgaagga aactacatta
    40081 agtttccgaa cggggtgaag aagtacatta aattctgcca ggaagaagat aaatccacca
    40141 accgcccgta tacctcacgt tatatcggtt cactggtcgc ggatttccac cgtaacctgc
    40201 tgaaaggcgg tatttatctc tacccaagca ccgccagcca cccggacggc aaactgcgtt
    40261 tgctgtatga gtgcaacccg atggcattcc tggcggaaca agcgggcggt aaagcgagcg
    40321 atggcaaaga gcgtattctg gatatcatcc cggaaaccct gcaccagcgc cgttcattct
    40381 ttgtcggcaa cgaccatatg gttgaagatg tcgaacgctt tatccgtgag ttcccggacg
    40441 cgtaatctcc tgcgcctctc cacacggaga ggcgttttta tcgctgcgca atgttgactc
    40501 ttgtcactgg cgaactctga cgattctccc acagcaccgt taacccacgc tgtaatgcta
    40561 taaaaataaa caacaaaata ccgatggcga ttttcgtcca ccaggaactc agcgtgccgt
    40621 caaagttgat gtaagtctgg atcagtccct gaattgccac gccaaaaagc gtcccaagca
    40681 ccgtcccgac gccaccactt aacaacgtac cgccaatcac caccgacgcg atggcgtcca
    40741 gttccacacc tacgcccgcc agtgcatatc cggcctgggt ataaatcgag aagacaatcc
    40801 ccgccagtgt tgccagtccg gtggagagca tataaatgcg aatagtggtg ctgcgagtag
    40861 aaatccccat caggttcgcc gacgtcgcgt tgccgccaat agcgtacacc tgattgccaa
    40921 aacgggtacg atgcgcaagg aaaataccga taaccaccac cgccagcatc aacagcccca
    40981 tcgcactaag gcgaccgccg ccggggattt tccacgcaag gcttgagagc gtgtcataaa
    41041 tgggatggtt tatcggaatc gactcttccg aaacgagata gctgacgccg cgcaaaaaga
    41101 acatccccgc aagcgtaatg ataaatgccg ggatcttcag ggcgtcgatc agaagcccca
    41161 taaatgcgcc gaaggcacag cccatcacca gcactagcgg aaacgccagc agcggcgaga
    41221 ggccgaaatc gccaaccact tttgccagaa acacgccagt aaaggcgatc accgaaccga
    41281 cggagagatc gataccgcca gagaggatca caaaggtcat gccaacggcg attatcccaa
    41341 gaaaggcgtt atctgtcagg atattgcaga tcactcttgt ggaagcaaaa ccgggaaact
    41401 gcgtcaggca gtagaaatag cccagcacaa agacgccgat ggtgatcatc agtggcaaat
    41461 tacgttttat catggctacg tactccttta atcagactga taaagcgttg cgactgaaca
    41521 atcagcacac aaagcaccac caccgctttt acgacctggt tcatctccgg cggaaagcca
    41581 gaaagtaaaa ttccggtgtt catcccctga ataatcagcg cccccacgac tgagagcagc
    41641 aggttaaagc gcccgcccat cagcgatccg ccgccaatca ccaccgcaag aatggcgtcc
    41701 agctccagcc ataacccggc gttattggca tcggcaccgc gaatatccgc cgccacgata
    41761 atgcccgcaa tcgccgcaca cagcccgctc aacacgtagg tgagcatgac gatgatccgc
    41821 gtgtttaccc cggcattttt tgccgcccga atgttgatac caacggcttc gataaacatc
    41881 cccagcgccg ttttgcgggt caacagccag aacaggagaa gcgtcagcac cgcgataatg
    41941 accggcgttg gcaggaacaa cagcgatccg ctgccaaacc atgagagatc cggcgagtta
    42001 aacgtgacga tctgcccggc ggtgatcagt tgcgccacgc cgcgcccggc gaccatcagg
    42061 atcagggtgg cgacaaacgg ctgaattttg aggatcgcta ccagtatgcc gttccacaat
    42121 cccgccagga tgccggtgcc cagggcgctt aacaaaacaa tcggcaggct gaatcccgcg
    42181 accgtcatcg cagccgttgt ggctccggcg atagccatca ccgcccctac ggagagatca
    42241 atcccaccgg tggcgatcac cagcgtcata ccaatcgcca gtaacgcaac gggagctgca
    42301 cggttaagaa tgtctatggg gctaccgaac aaacgcccat cctgaagcac tacctgccag
    42361 aaatgcgggg ctaccaggct gtcgaccagc agcaccagca atagtgctac cagctgcgac
    42421 attcccgttg gccagcgaaa gcgccttttc ggcggcgtag tgtccgggag agattgaggc
    42481 atcacactgt tctccttacg ccgcaatggc gttcatgatc gccggaacgg aaagctctgc
    42541 cagcgggatc tccgccacct gtttgcgatc gcgcatgatg atcacccggt cggcatagcc
    42601 caccagttct tccagttcgg aggagatcac cagcagcgcc agaccatcgg cgcatagcgt
    42661 ttcaatcagg cggatgatct cggcgtgggc accaacatca atgccacggg ttggctcatc
    42721 gaggatcaga aattgcggac gagtcagtag ccaacgtgaa agcaatactt tttgctgatt
    42781 gccgccggag agaaattcaa tcggttgttc agttgaaggt gtgcgaatgc caagctggcg
    42841 gataaagcgt tcggcaattt cttgctgttc tttacgggaa atgggtcgta gccagccgcg
    42901 ctgggcctgg agagcgagga tgatattttc ccgcaccgag gcggcagcga tgatgccatc
    42961 ggttttcctg tcttccgggc agaagccgat gcccagtacc gaagcctgat gtggcgatcg
    43021 cagggtttgc ggtttgcctt tgatcaacgc cgtgccgctg tcagcaggtt tgataccgaa
    43081 gatcacttcg gcggtttcgg tacgtcctga tcccagcaag cctgccaggc cgacgatctc
    43141 gccggggcgc acttcgagat caaacggtgc gatcgttcct tttttgccgt aatttttgaa
    43201 cgcggcaacg ggtttgtcgc tcaacaatgt tcgcccggca cgctgtagcg cgtgggtatc
    43261 cagctcgcgc cccagcatca tttttaccag ttcgatctgc ggtagctcgc gcgtttcccg
    43321 acagcctacg aaactgccgt tgcgtaagac ggtgatccga tcgctgacct gatagacctg
    43381 atcgagaaag tgagtgacaa agatcaggct gacgccgcga tcgcgcaact gacgcatcag
    43441 gccaaacagt aattctactt cctgagtgtc gagactggcg gtgggttcat cgaggatcag
    43501 cactttggca gagagatcga tagcccggca aatggcgacg atttgctgca tcgcgactga
    43561 aaagcggtta agcggttcac gcacgtcgag ggagaaaccg taagatgtca tcagttcggt
    43621 ggcacgcttt tccatctctt tgcggcgtag aaagccgaaa cgtttgggtt cgcgacctat
    43681 gaatagatta tcagcgaccg acatattggg tagcaggttg acttcctgat aaacggttcc
    43741 gatgccgagt tgttgtgcat gggcggtatt tttcggtgag atagcctggc cttccagcca
    43801 gatggtgccg cgatcggcgt ggtatacacc ggttaatgct ttgattagcg tcgattttcc
    43861 cgccccgttt tcaccgagca gcgccatgat ttcgccacgg cgcaggctga aatcaacgtt
    43921 gtctaacgct ttgacgccgg ggaaaaattt acttaatcct tcggtgcgga ggatttcctg
    43981 gtgttggtcg gtcgtcatgg tttccccctt agccacgctc taaaagtctg ggaatagatc
    44041 gtgcctgatg ttaagagctg cgccgtttgt ccccctcacc ctaaccctct ccccgaaggg
    44101 gcgaggggac agattgtgca cggtgttaag tccggtgttg caccactgcc ggatgcggcg
    44161 tgaacgcctt atccggccta cacagagcac cgaactcgta ggcatgataa gacgcggtaa
    44221 gcgtcgcatc aggcttcagc gcaccaccgc
//
LOCUS       DAMZET010000019        41171 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.019, whole genome shotgun sequence.
ACCESSION   DAMZET010000019 DAMZET010000000
VERSION     DAMZET010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.019"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..535)
                     /locus_tag="POB34_001159"
     CDS             complement(<1..535)
                     /locus_tag="POB34_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDI5918147.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDT"
     gene            complement(646..2058)
                     /gene="ftsP"
                     /locus_tag="POB34_001160"
     CDS             complement(646..2058)
                     /gene="ftsP"
                     /locus_tag="POB34_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HDI5918148.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(2133..2870)
                     /gene="plsC"
                     /locus_tag="POB34_001161"
                     /gene_synonym="parF"
     CDS             complement(2133..2870)
                     /gene="plsC"
                     /locus_tag="POB34_001161"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HDI5918149.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(3104..5362)
                     /gene="parC"
                     /locus_tag="POB34_001162"
     CDS             complement(3104..5362)
                     /gene="parC"
                     /locus_tag="POB34_001162"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDI5918150.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(5500..7107)
                     /locus_tag="POB34_001163"
     CDS             complement(5500..7107)
                     /locus_tag="POB34_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI5918151.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYYALLNQATQITDATKRNALYQQAEVIINQQTPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(7240..7635)
                     /gene="mqsA"
                     /locus_tag="POB34_001164"
     CDS             complement(7240..7635)
                     /gene="mqsA"
                     /locus_tag="POB34_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MqsA"
                     /protein_id="HDI5918152.1"
                     /translation="MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESI
                     MNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKKLSLTQKEASEIFGGGVNAFSRY
                     EKGNAQPHPSTIKLLRVLDKHPELLNEIR"
     gene            complement(7637..7933)
                     /gene="mqsR"
                     /locus_tag="POB34_001165"
     CDS             complement(7637..7933)
                     /gene="mqsR"
                     /locus_tag="POB34_001165"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin MqsR"
                     /protein_id="HDI5918153.1"
                     /translation="MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCN
                     VIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQVYLKITVIHDVLIVSFKEK"
     gene            complement(8138..8620)
                     /locus_tag="POB34_001166"
     CDS             complement(8138..8620)
                     /locus_tag="POB34_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI5918154.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(8673..9065)
                     /gene="ygiW"
                     /locus_tag="POB34_001167"
     CDS             complement(8673..9065)
                     /gene="ygiW"
                     /locus_tag="POB34_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HDI5918155.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            9217..9876
                     /gene="qseB"
                     /locus_tag="POB34_001168"
     CDS             9217..9876
                     /gene="qseB"
                     /locus_tag="POB34_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HDI5918156.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPDKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            9873..11222
                     /gene="qseC"
                     /locus_tag="POB34_001169"
     CDS             9873..11222
                     /gene="qseC"
                     /locus_tag="POB34_001169"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HDI5918157.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNRLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            11332..11913
                     /gene="mdaB"
                     /locus_tag="POB34_001170"
     CDS             11332..11913
                     /gene="mdaB"
                     /locus_tag="POB34_001170"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HDI5918158.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            11944..12258
                     /locus_tag="POB34_001171"
     CDS             11944..12258
                     /locus_tag="POB34_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDI5918159.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(12302..13633)
                     /locus_tag="POB34_001172"
                     /pseudo
     CDS             complement(12302..13633)
                     /locus_tag="POB34_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778885.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13920..14672
                     /locus_tag="POB34_001173"
     CDS             13920..14672
                     /locus_tag="POB34_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI5918160.1"
                     /translation="MIRIYKASKGQLKHREIFDEIKKKIDNGEWKEGKAIPTERELAE
                     HFLSSRTTIRKAIESLKQRGYLHSVHGQGTFTLPARSRENHLLHSFTDDIKARGGVPA
                     QNILEIGYIPLSDIIRKNLELDIHVHTVFCIKRIRYMGSTPLGIQTSWLALNDGQEIT
                     QEELLATGSLYILLEEKLAIKPLEATEIISARLPSPLERQLLELTADDVVLSCTRVTL
                     SVERKPMEYVEMVYPASRYSYKARITKDSFSV"
     gene            14686..15762
                     /locus_tag="POB34_001174"
     CDS             14686..15762
                     /locus_tag="POB34_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDI5918161.1"
                     /translation="MNKIEQAVIYNKILGSLACAGMGDALGAATELYSIDEIKAQWGG
                     FLNAFVSPPADTFAGSLNGIAGLITDDSSQMYVFSEGLVEAGFDNFTNNDWLACLLRW
                     ADMQPYANYKGPTTEQIVKALKEGRPTNTIGRIGTSSRQAPNVGTTNGAGMRVAPAGL
                     IWPGKKEKACHLALITCLPSHDTNIAIASACAIAAATSQAMLPEASLTSLLDAAIWGA
                     NYGERLAKQYARCVAGPSIAMRIQLAADIARRANDLESCLREMEGLVGNSVAAHESIP
                     AAIGLLLYCKGEPWETIHACANIGNDTDSIATMAGAIAGAWRGFDALPEDKYAFFRAV
                     NNKDFDIEAIASGLTLLALQAQEK"
     gene            15762..16760
                     /locus_tag="POB34_001175"
     CDS             15762..16760
                     /locus_tag="POB34_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDI5918162.1"
                     /translation="MNMPARTPVMPLAQTREEINATAASLRHLAAIWGECFSTFQLDK
                     RAVERVCIVGSGDSWTVALCAAAWLGKYTHLFCYALQTWDFLQTDLTRYQKETLIIIL
                     SASGRPSMTVDALCHAVCSNAQVLGVTNCPGTPFCAITENMLYTWANKQGIPTQSSSV
                     TLYALLRLAQKLCPGLTPLQIEEDLEGKFSQINQHWQQKKRHFYQQKEITFLGSGLSW
                     GLAISGSNLLSCGPQIRATALPLEEFYHSLRLHQAGVGQHYILLPATSCESPFYLATQ
                     KAIVEQGATAELISFIPDASESNNLFLVMQWLYEMCWHLSCDYVDAGGQRVSHREK"
     gene            16765..17679
                     /locus_tag="POB34_001176"
     CDS             16765..17679
                     /locus_tag="POB34_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HDI5918163.1"
                     /translation="MKKFDVAALGSGNIDIFLSIPSLPTRGGKVVGTRLGEQAGGTVA
                     NSACAMGQLGLNVVSVSCVGNDHSASIILDGFKKYHVNCDFVQVIPELIANTAIIFID
                     ELGEKKLVYSPGSDHEWDKEKALQAIAQSRYFYTMPADIEKFRMLAEYSRSQMTKVVV
                     DIEPHIIATPEHLARILHLADIAIFNYDGFIRGYATEPDFTLLHDIQDEYQLDAVVVT
                     LDVRGVIAVKGNEQVKIGSYTIPVIDTTGAGDTFNGAFVYSLIKNMPLIEALKFASAT
                     AAINITALGARGHLPTPSEVNLFLSQHD"
     gene            17707..18831
                     /locus_tag="POB34_001177"
     CDS             17707..18831
                     /locus_tag="POB34_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HDI5918164.1"
                     /translation="MKIRYAAIFLALPLLCTTAQANSFLHYAGEFNFRTGEKQNNVTI
                     AGLSAITFDAKKNIFYAINDSRNNNKEGDASLYTLKIQVSSRGIEQVNFLNQRPLLDA
                     KQQPFVTNTVDAEGLALTHNGKSLLWSSELGAPLRLSTLDGVMEKDFTSLFPARFNIS
                     SDKESSNGIRSGNAWEGLTVTPDGKSLFIAVESSLKQDGPIASPINSGTARLLQFSID
                     ADGRPSKQLHEYLYITDPVPQVSKFGINDNGVSEVLALNDHQLLVIERSGRNVSAGFN
                     DWDYSVRVYMVDLTAASDIKNIDSLQDWSNKSTLQPVSKKLLIDFADYTSSADCIEGV
                     TFGPLIDGHTSLIFVSDNNFQPHQQTKFYLFIDKENKLKI"
     gene            18966..19661
                     /locus_tag="POB34_001178"
     CDS             18966..19661
                     /locus_tag="POB34_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDI5918165.1"
                     /translation="MLSCLSPTLVCAEQPHGFIDYRHEYLDDTRTHADRVEFGTFFSN
                     GIGLMGELRYNTDEGDKDKWDPSQFGNNGTGLSVVYRFKPLDDKKFWLEPMFWLDTTQ
                     YWSTYEYGLSAGYDFSKEWKVSGRFRYDMDKATDKSKGYGNDDRNNRRYDVWIDYRPQ
                     KTNFQYQLNLVYYNNGYLTWNDGHKNYTASFKVGYKIGSWIPYMSIADYKGVDKTSSN
                     RQIRWRWGLTYTF"
     gene            19675..20544
                     /locus_tag="POB34_001179"
     CDS             19675..20544
                     /locus_tag="POB34_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2608 domain-containing protein"
                     /protein_id="HDI5918166.1"
                     /translation="MNNKFKIILWMMLSALPALSYAQVKNIVTGEYSAVSKIITEKET
                     LYPSDKILLVFDIDNTLLTSGTRIGGDIWYQWQTDKLPLKPDDSQKVPCLYENTISML
                     YELSPMQLTEPQLPTMLKQWQQKHTAFALTSRAPDTLFPTLRELKRNGIDFSSSALRK
                     KEDTLPPFEKGKLKRSWLYSQGVFFSSGQDKGVILDYMLDKMGNKYDAIVFIDDGQAN
                     IHAMTKMFSKDKWFSTDFTIIHYTRVEDELIKQQGAVITTAQAEKMATDWKTLAGSLA
                     TLFPDRNKLCPIK"
     gene            complement(20589..22481)
                     /gene="parE"
                     /locus_tag="POB34_001180"
     CDS             complement(20589..22481)
                     /gene="parE"
                     /locus_tag="POB34_001180"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDI5918167.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(22510..23091)
                     /gene="yqiA"
                     /locus_tag="POB34_001181"
     CDS             complement(22510..23091)
                     /gene="yqiA"
                     /locus_tag="POB34_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HDI5918168.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(23091..23918)
                     /gene="cpdA"
                     /locus_tag="POB34_001182"
     CDS             complement(23091..23918)
                     /gene="cpdA"
                     /locus_tag="POB34_001182"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HDI5918169.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(23943..24365)
                     /locus_tag="POB34_001183"
     CDS             complement(23943..24365)
                     /locus_tag="POB34_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HDI5918170.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(24366..24995)
                     /gene="nudF"
                     /locus_tag="POB34_001184"
     CDS             complement(24366..24995)
                     /gene="nudF"
                     /locus_tag="POB34_001184"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HDI5918171.1"
                     /translation="MLKPDNLPVTLGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            25200..26681
                     /gene="tolC"
                     /locus_tag="POB34_001185"
     CDS             25200..26681
                     /gene="tolC"
                     /locus_tag="POB34_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HDI5918172.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            26829..27500
                     /locus_tag="POB34_001186"
     CDS             26829..27500
                     /locus_tag="POB34_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HDI5918173.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            27506..28666
                     /locus_tag="POB34_001187"
     CDS             27506..28666
                     /locus_tag="POB34_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDI5918174.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(28704..29492)
                     /gene="ygiD"
                     /locus_tag="POB34_001188"
     CDS             complement(28704..29492)
                     /gene="ygiD"
                     /locus_tag="POB34_001188"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HDI5918175.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGTL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVEGIEMGSLSMLSVQVG"
     gene            29635..30408
                     /gene="zupT"
                     /locus_tag="POB34_001189"
     CDS             29635..30408
                     /gene="zupT"
                     /locus_tag="POB34_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDI5918176.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(30466..30636)
                     /locus_tag="POB34_001190"
     CDS             complement(30466..30636)
                     /locus_tag="POB34_001190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918177.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(30897..31550)
                     /gene="ribB"
                     /locus_tag="POB34_001191"
     CDS             complement(30897..31550)
                     /gene="ribB"
                     /locus_tag="POB34_001191"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HDI5918178.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVDQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(31654..31802)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            31924..32214
                     /gene="ubiK"
                     /locus_tag="POB34_001192"
     CDS             31924..32214
                     /gene="ubiK"
                     /locus_tag="POB34_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HDI5918179.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
     gene            32497..33048
                     /locus_tag="POB34_001193"
     CDS             32497..33048
                     /locus_tag="POB34_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HDI5918180.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PCDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPSKDATPGNVYAQADYKIAYE"
     gene            33108..35612
                     /locus_tag="POB34_001194"
     CDS             33108..35612
                     /locus_tag="POB34_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDI5918181.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            35628..36377
                     /locus_tag="POB34_001195"
     CDS             35628..36377
                     /locus_tag="POB34_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDI5918182.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            36379..37443
                     /locus_tag="POB34_001196"
     CDS             36379..37443
                     /locus_tag="POB34_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI5918183.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNMVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(37486..37686)
                     /gene="glgS"
                     /locus_tag="POB34_001197"
     CDS             complement(37486..37686)
                     /gene="glgS"
                     /locus_tag="POB34_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HDI5918184.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            37955..38584
                     /locus_tag="POB34_001198"
     CDS             37955..38584
                     /locus_tag="POB34_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HDI5918185.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            38611..40272
                     /locus_tag="POB34_001199"
     CDS             38611..40272
                     /locus_tag="POB34_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDI5918186.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            40511..40570
                     /locus_tag="POB34_001200"
     CDS             40511..40570
                     /locus_tag="POB34_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDI5918187.1"
                     /translation="MMKLVIILIVLLIVSFAAY"
     gene            40887..40946
                     /gene="ibsE"
                     /locus_tag="POB34_001201"
     CDS             40887..40946
                     /gene="ibsE"
                     /locus_tag="POB34_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HDI5918188.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
BASE COUNT        11131 a        10047 c         9663 g        10330 t
ORIGIN      
        1 cggtatcgga ccagtaatca taatgtgcgg tacggcgcag actagcctca ataccgccga
       61 ggatcaccgg tacatctttc cacgcctctt tacaacgctg ggtataaacc agtgtggcgc
      121 gatccgggcg cttacccgcg acgttatccg gcgtgtaggc atcgtcatga cgtaaacggc
      181 gatcggcggt ataacggttg atcatcgaat ccatgttgcc agcagtaaca ccgaaaaaca
      241 gattcggttt acccagacgc atgaaatcgt ctttgctgct ccagtctggc tgggcgatga
      301 tcccgacgcg aaagccctgt gcttccagca tacgaccgca aatcgccatc ccgaagcttg
      361 ggtgatcgac atacgcgtcg ccagtaacca aaatgatgtc gcagctatcc cagccaagtt
      421 gatccatctc ttcacgagac atcggcaaaa acggtgccgg tccaaaacag gcggcccagt
      481 actgcggcca ggagaacagg tcgcgatccg gttggatcag ggagatagag ctcattttgc
      541 ttccagaaat gataaaaaaa taatcaaagg ccggggatta taagccggaa cgaaagagaa
      601 atcgaaaggt attccatact cgccctcctc gggcgagtat gaagattacg gtaccggatt
      661 gaccaaaagt tgccctatcg agccacggtc cgccatttcc agcgtctgac tgttgaagta
      721 gaacgggaag tgcgcccagg aaggctgacc gaaataaaca agcagctcca cttgtccgtc
      781 aacccaaacg gtatctttcc agcctctgtc ttccgggaac ggcatcgcgc cattcacgtt
      841 acggatctgg aacattacgc cttcaatatg gaacgcttgc ggctcgtccg cgcgtaccgt
      901 ccagcgttcc cacgttcctt gctgcgcggt gacatcaata cggttgacgt cccacagttg
      961 tccattaata cccgggtcat cacccagact gatatcgcga ctgcgaatcg gcgaaccggc
     1021 catgatttca gttggcagca agcgcatcgg aagactgtct gtgaccagcg gcagaaggcc
     1081 ggttgggcgc agcgttagca ccagggtaga aaccagaatg ctggatggct caaagaagcc
     1141 acgaatacga tcaacaatgc tcgccgcttc gccacaggtg atcgacactt catcgccgtt
     1201 gctcatatcc accagaatct cgcggcgctc gcccggtgcc agcgaaagtt gcttcaccga
     1261 tacaggagca ggcaggaatc cctgatcgcc agaaatcaca tgtaacgggc gaccatcgct
     1321 catctgtagt tgataacgac gagagttcga cgcgttcagc aggcgcaagc gcacccagcc
     1381 acgcgagact tcaacgtacg ggctttgtac accgttaacc agcagcgtat caccaacaaa
     1441 gccgccgctt cccggttcgt tgtattctgg cgtaccaaag ttatccagcc gtttatcctg
     1501 aataatgacc ggaaaatcat ccacaccata atggttgggg ataggcagcg acttgctgac
     1561 ttcatcttcc accagccaca ttccggcaag gccgttatag acctgctgag ccgtgcggtt
     1621 gggagtattg gcgtgatacc acagagtagc tgcgttctgg cgaataggca gtacgggtgc
     1681 ccagtcagcg tttggcgaca tcatccgtgc cggaccgccc atcagcgggc ctggtacctg
     1741 tagcccggct accgtcattg agacattttc tgtcaggcgg ttgctgtaaa taagcttaac
     1801 atcgtcgccc ttccagacgc ggatagtcgg ccccaggtaa cgaccattga ttccccagac
     1861 cgacgcgcgt gtccctggcg taaatgacca gtgcgcacgt tgtacagtca taaacagcgg
     1921 ttgcccacgg cgagattcaa gcagcggcgg aacgggtagc ggttgctgtt gcccggctgc
     1981 gctggccttc aggggaacag cgcctgcaca aagtgcaatc cccgatgcct gaatgaactg
     2041 acgccgactg agtgacatat ttgctccatg taaaactggc taaagtaata ccgtgcagga
     2101 aagtgttccc cgcaaaaata cagattgccg acttaaactt ttccggcggc ttcgcgttct
     2161 gcgacttctt tatcgagctc ggcgattttt tgttccatta tcgaacgaca atgggcagcc
     2221 agctcacgaa cctgatcttt gccatactga ctgacgtcaa ttggcggcag catttcgaca
     2281 atcaccagac cgttgtgcag tcgattaaga ttaatcttat tcgaagttgt agagacgcac
     2341 acgggaataa tcgggacgcc cgccgcaatt gccgcgtgaa atgctccagt cttgaacggt
     2401 agcaggccgc gaccacggct gcgggttcct tccgggaaca tccagatgga aatacggcgt
     2461 tttttgaaat gattcacgac ttccgcaatg gtgccgtgag ctttagtgcg attgtttctg
     2521 tcgatcaata agttgccggt taaccagtac aactgcccga agaaggggat ccacagcaag
     2581 ctctttttac ctaccgtcac cgtcggcggt tgcacaatgt tcgatgctgt caccatgtca
     2641 tagttgttct ggtggttagc gatatagata gcattgccgt agctttcagc atctgcaggt
     2701 ttgcggcact caactttcag gccgaacagc ggcgcaagac ggccaaacat atgcccaaag
     2761 gtggccacat gtttcgggtt acgcgggctg aaaaggcagt aaatggagcc gaatacacag
     2821 actaagatgc tgtaaatcac ggtaataata agacgaaaga tatatagcat aacaccctct
     2881 gaaggtctga gagactggca ttgtacgtca cctgaaatca ggttagggca ttgttaaaag
     2941 ccgctctctg gaaaagcgta ttgttaatcg caacgcatga ataaacaacg gtttcaaggg
     3001 aatttttatc gtttccgggg gttgaatacg ctgccggatg acgacttaac gtttcatccg
     3061 gcgttccttg caagcgggag gaaacagcgc cctccccggc atattactct tcgctatcac
     3121 cgctgctggc acggcgagga gagtcgatct caacacgatc gatacgctgc aaaccgcgca
     3181 tcaacgtacc gcggcgtcca cgttcgccag tgactttctg tagctcttcc ggacgcagtt
     3241 taattttgcg tttcccaaca tgaatggtca gcgtgctttg cggcggcaga acgtacaatt
     3301 gcgccagacc atcttctcca cgcgcggctt ctgccgatgg aatgttgata atcttgttgc
     3361 ctttgccctt cgacagctgc ggcagatcgc ttaccgggaa catcaacata cggcctgcct
     3421 gagtgattgc cagcagcata tcggaagcat cttcaatcac caccggcggc ataacatggg
     3481 cattttccgg taaggtgatc aaagccttac ctgcacggtt acgcgccacc agatcgttaa
     3541 aggtgcagac gaaaccgtaa cccgcatcgg aagccatcag cagtttctga tcgtcgcttt
     3601 ccatcagcat atggtcaacg gtcgccccag gcggcaacgt taatttgccg gtgagcggct
     3661 cgccctgacc acgcgccgac ggcagcgtaa tcgggtcaat ggcatagcta cgaccggtgg
     3721 aatcaacaaa cactaccggt tggttgctct tacctttcac cgccgctttg aagctatcac
     3781 ccgctttata attcaggccc ggcgcgtcga tatcatggcc tttagcgctg cgtacccagc
     3841 ccatctgcga cagcacaatg gtgacaggtt cagacggcag catgtcgtgc tcgctcatcg
     3901 ctttcgcttc ttcgcgttcc tgcaacggcg aacgacgatc gtcaccgtag gcttgcgcgt
     3961 ctgcctgcag ttctttcttc agcaggttat tcattttacg ctcggaagcc aaaatgccct
     4021 gcaactggtc gcgctctttt tccagttcac tctgctcacc gcgaatcttc atctcttcca
     4081 gtttggcaag atgacgcagt ttcagttcga ggatcgcttc cgcctgggtt tccgtaaggc
     4141 caaaccgcga catcagcgcc ggtttcggtt catcttcatt acgaatgatc tcaatcactt
     4201 cgtcgatatt gagaaacgcc accagcaaac cttcgaggat atgcaggcgc ttgaggactt
     4261 tctccagacg atagttcagt cggcggcgca cggtatcgcg gcggaacacc agccattcgg
     4321 agaggatttc cagcaggttt ttcaccgccg gacgaccatc cagaccgatc atattaaggt
     4381 taatacgata gctcttttcc agatcggtgg tagcgaagag gtggttcatc acctgatcca
     4441 tatccacgcg gttggaacgc ggcacaatca ccaggcgggt cgggttctcg tggtcagatt
     4501 catcgcgcag atcgtcaacc atcggcagct ttttgttgcg catttgcgca gcaatttgct
     4561 ccagtacgcg cgcacctgaa acctgatgcg gcaatgcgct gataaccacc gcgccatctt
     4621 ctttcttcca caccgcgcgc atacgcactg aaccacgtcc gttctcgtag attttacgga
     4681 tctcggcgcg cgaagtgata atttccgctt cagtcggata atccggcccc tgcacgatat
     4741 ccagcagctg atcgagcgtg gttttcggct ggtcgattaa tgcgattgcc gcctgagcca
     4801 cttcacgcag gttatgcggt ggaatatcgg tcgccatgcc gacggcaata ccggtggtgc
     4861 cgttaagcaa aatgtttggc agacgggcag gtagcatttt cggctcctgc aaagtgccgt
     4921 cgaagtttgg tacccagtca gccgtcccct gccccagctc gctcaatagc agctcggaat
     4981 atttcgacaa ccgggattcg gtgtaacgca ttgccgcgaa cgatttcgga tcgtccggcg
     5041 cgccccagtt cccctgacca tcaaccagcg gataacggta agagaacggc tgcgccatca
     5101 ggaccatcgc ttcataacag gcgctatcgc cgtgcggatg gtatttaccc agtacgtcac
     5161 cgacggtacg ggccgatttt ttaaatttgg cgctggcatt caggcccagt tcagacatcg
     5221 catacacaat gcggcgctga acaggtttca gaccatcacc aataaacggc aacgcacggt
     5281 ccatgatcac gtacatggag tagtttaagt aggcgttttc cgtaaattca tgtagcgcaa
     5341 ggcgctctgc catatcgctc attaattctg attcctcaac ttattcgcca gccttcaaag
     5401 gggcaatatt gccggagata ctacattatc tgccgcgtcg agtcacagga gatcagcgtg
     5461 aatagcgccg ccggacgtgc tccgacggcg ataaaggcat caatgtgcct tgatatacaa
     5521 ctctttgctg tagacataat cctggggatt atgcagcgga aaaccgccaa cgtagggttt
     5581 aagcagtttg attaacggct gatagtagat aggaatcagc ggtgtctgtt ggttgatgat
     5641 cacttctgcc tgctgatata acgcattacg ctttgtcgca tcagtgatct gcgtggcctg
     5701 gtttagtaag gcgtaatact gcgcattttt ccagtgaccg acgttttctt cactatcgct
     5761 tttgagcgtg ttcaggaagc tggaagcatc attgtacgtc gcatcccacg actgccgaga
     5821 cagcatgaaa tcaccggctc gtcgggcatc aagataggtt ttccactcca ttgtgcgcag
     5881 cgtcacctgt gcacccagcc attttttcca ttcggaagac aacgctatcg cggtcttttc
     5941 atgcagatcg tacttgttgt agaacagctc aaagcgtagc ggatgagagg cgtcgtatcc
     6001 cgcctgtttc agcaaggctt ttgccatcgc gacgcgctca ctcattggct tttgcagttc
     6061 atcgaacgtc gtcgcgctaa agccttttac ctctggcggc gtcagcgtgg ttgcgggcgt
     6121 tctcaacccc agtacctttt gcgcaataag ctgtcgatca accgtaagat atagcgcccg
     6181 acgcactcgc acatcgttaa atggcggttt ctcaaggttg aagttgtaat attcgctgtt
     6241 cagacgcgga ataattcgta gctcgccagg cagtgatttt tcaatggcgg gaatttgctg
     6301 cgccggaacc caggtgagat cgacctctcc cgcgcgatag cggttatagc cggtgaccga
     6361 attatctagc gccagatact caacctgttg caatactgta tgttgcgcat cgcggtactt
     6421 tggatttttg cgtgcagtaa tcttttcgtt aactacccac tgatcaagca caaaggcacc
     6481 gttgtaaacc atgttctctg gcttactcca gctatcgcca tgtttagcga tgacatgatg
     6541 aggaaccggg aacagcgtcg gccaggcgag catcgtcgtg aaccaaggaa ccggctgctc
     6601 aagcgtaact tcaagagtac gatcatccgt cgctttgaca cccagcgatg taacatctgc
     6661 tttacccgca acaatagctg cggcattgtt aatgtgcgcc tgtgccagat agccagcaaa
     6721 agggcttgcc gttttcgggt caaccgcgcg ctgccagccg aggacaaaat cctctgccgt
     6781 cagaggctga ccgtcggacc actgcaaacc gctacgcaga tggaaaatat agcgcttgcc
     6841 gccgtccagt atctcccagc gttcagcctg agcgggctgc acctggcctt caccgtccat
     6901 ccataccaga ccttcaaaca gatccagcac aatctgcgcg gcagtattct cctccacctt
     6961 ttgcgggtcg agcgtacctg ggtcgctatg attgttgtaa cgaaagactt gttgcggggc
     7021 gagcggtgtg ttggcgggaa cgtcagcagc gtagagagga gccgcactta ccaggctgac
     7081 cagccataat aaatttcgcg tatacatggt attccttacc ggagaaatta taagcccaac
     7141 aacaatacgc ctgtggcatt gttcgctcaa acttatcgcg agtgatttgg ctcacactcc
     7201 ggtaaagaaa aaagcgagtt gcccccgctt ttccattaat taacggattt cattcaatag
     7261 ttctggatgc ttatccagaa cacgtaaaag tttgattgtg gaaggatgag gttgggcatt
     7321 gcctttttcg taacgcgaaa acgcatttac acctccccca aaaatttcgc ttgcctcttt
     7381 ttgggtaaga gagagctttt ttcgaacctt cactataaat tcaggtgcca ctgtttcggc
     7441 attcaccgaa gcccgaaatg cctttacttg cgccatgaaa gcatctgact cttctttatt
     7501 catgatgctc tcttcgcaat ggacacaata taaaccgtgg atacctttca atactgtttt
     7561 tcgtccacgg aaggtgtatg gaatatcttt aatgccagaa accatttctc cctggtggca
     7621 aaccggacat ttcatattac ttctccttaa acgagacgat cagtacgtca tgaattaccg
     7681 taattttaag ataaacctgg cctgtaacaa gcctgggtct gtaaacatcc tgccagatag
     7741 tatgatcaga gtaggtggtc atgcttttat aaaagtcgct ctctgataat ccaatgataa
     7801 cattacacat accatcaaaa tccaaaccta actcatctgc atttaacagt gcactacgtg
     7861 ttgtacgaac ttgcccggca ttgacaagtt ttttaacctg actcaaacgt gtatgtggtg
     7921 tgcgtttttc cataaccccc gcctccctgt tactttagtt ataacctaaa aggttaatta
     7981 cagcaatgaa aaaccaccta aaaggttagt tagatgtacg gatatagtga ccacacaaaa
     8041 cgtattcttt aaggaaagtg attgaccata taagaaagtg gcgcattagt agcgccagtt
     8101 tgaagcagga atttataagg gaagctggag tcaggcatca cgctaacggc acataaacat
     8161 ccgtttccag tcgctgctct gtcacccctt cggcaagatt ggtgtagtga aacagaatcg
     8221 gtgctttacg cattttctcg ccgcttgcag gcagccagtg gcgaataatg ccccatatcg
     8281 tgtgtgaaat atcgtccagc tcgccaacgt ggcgggccac ggcataacgt ccaccggtaa
     8341 gttcaccatt gctcacacca taacgattat cgggaatcgg ttcgctaacg ctgccgcaga
     8401 tatcaaagcg aaacgcttcc ggtgcggtgg tggcagggtc gtcccaggcg acgccaaaag
     8461 tctggctttg gttaacagga gaaagccccg tctctttacg ccacatgata aatttcgcca
     8521 cactgtagtt gagcaactca gggctacagc ggtgcggcaa catcgccaca gggattgatg
     8581 ggaaatcgat aatgtttaca tccagtgtca ggtttgtcat tttgcctccc tgcgaaatta
     8641 ttcccgggaa cgcgtcccgg gagcagtaac aattacggat ttactttgcg gatctgtttg
     8701 acgtcaattt caacagaatt ccagtcttta tcgacttcac cctgaatctc aaccgtatct
     8761 ttcggcgtca ccgtcacgcc gttccagcgt ttgtgatcga tatcaacatt gatagtaccg
     8821 ctggcatctt tgaacacgta gagatcgtca gagatgcgtt caacgatatt gccgcgcagg
     8881 gttacccagg tgtcgtcacg cagggatttt gcgctttcta cagtcgttac gctgccgttc
     8941 ggcccctgga atcctccggc ctgactttgc gttgccgatg ggccagaaaa accgccctgc
     9001 tctgctgcca tcaccggtgc gctgcacagg gccattactg cgattactgc tgcgaatttt
     9061 ttcatgttta ttactccctt taatgtctgt ttccgagcat ttaacaagat agtccttaac
     9121 aacttcttaa gggaaaaaaa taaaatttag tgctgtacag agcgcgttac aacacggttt
     9181 actggcagca aatacggtta tcgcagggat gaaaaaatgc gaattttact gatagaagat
     9241 gacatgctga ttggcgacgg catcaaaacg ggccttagta aaatgggttt tagcgtcgac
     9301 tggtttacac aaggtcgtca gggaaaagag gcgctttata gcgcacctta tgatgcggtg
     9361 atcctggatt taaccttacc aggcatggat ggtcgcgata ttttgcgcga atggcgagaa
     9421 aaaggtcagc gtgagccggt actgatcctg accgcgcgcg atgcgctggc ggaacgtgta
     9481 gaagggctgc gtctgggagc tgacgattat ctgtgtaaac cttttgcgtt gatagaagtc
     9541 gccgccaggc tggaagctct gatgcgccga accaacggcc aggccagcaa cgagctgcgc
     9601 cacggtaacg tcatgctcga ccccgacaaa cgtatcgcca cgctggctgg cgaaccctta
     9661 acactgaaac caaaagaatt tgccctgctg gaattactga tgcgtaacgc tggtcgggta
     9721 ctgccgcgca aactgattga agagaaactg tatacctggg acgaagaggt caccagtaat
     9781 gccgttgaag tgcatgtgca tcatctgcga cgcaaactcg gtagtgattt tattcgtacc
     9841 gtgcatggta ttggctacac attaggtgag aaatgaaatt tacccaacgt cttagtctgc
     9901 gcgtcaggct gacgctaatc tttttaattc tggcctcggt gacctggctg ctttccagct
     9961 ttgtcgcctg gaaacaaaca acggataacg tcgatgaatt gttcgacacc caactgatgc
    10021 tgtttgccaa gcggttaagt acgctcgatc tcaacgaaat caacgcggcg gatcgcatgg
    10081 cacaaacgcc aaatagatta aaacacggtc atgttgatga cgatgcgctg acctttgcca
    10141 tctttaccca cgacggcaga atggtcctta atgatggcga taacggagaa gatattccct
    10201 atagctatca acgggaaggt tttgctgacg ggcaactggt cggtgaagac gatccttggc
    10261 gttttgtctg gatgacctca cctgatggca aatatcgcat cgttgttggc caggaatggg
    10321 aataccgtga agacatggcg ctggcgattg ttgccgggca attgatcccg tggctggtcg
    10381 cactgccgat tatgttaatc atcatgatgg tactactggg tcgtgaactc gcgccgctga
    10441 acaaactggc gctggcacta cgtatgcgtg accctgactc ggaaaaacca ctaaacgcga
    10501 ctggcgtacc cagcgaagtg cggccactgg ttgagtcgct aaatcaactg ttcgcccgca
    10561 cacatgcgat gatggttcgt gaacgacgct ttacctccga cgcagctcac gaacttcgta
    10621 gcccgttaac ggcgctgaaa gtgcaaaccg aagttgcgca gctctctgac gatgatccgc
    10681 aggcgcggaa aaaagcactg cttcaattac attccgggat cgatcgcgct actcgtctgg
    10741 ttgatcaact gctcacgcta tcgcggctgg actcactgga taaccttcag gacgtcgcgg
    10801 agatcccgct tgaagatctc ctgcaatcgt cggtgatgga tatttaccac acggcgcagc
    10861 aggcgaaaat tgacgtgcga ctgacactca atgtccaggg catcaaacgc accggacaac
    10921 cgctattgct aagtttgttg gtgcgaaatt tgctggataa cgccgtgcgt tacagtccac
    10981 agggcagcgt ggtggacgtc acgctgaatg ccgataattt catcgtgagg gataacggcc
    11041 ccggtgtgac accagaggca ctggcgcgaa ttggcgaacg cttctatcgc ccacccggac
    11101 aaaccgccac cggcagtgga cttgggctat cgattgtcca gcgaatcgcc aagctgcatg
    11161 gcatgaatgt tgagtttggg aatgcggaac aaggtggatt tgaggcgaag gtaagctggt
    11221 aagggtaaga cttttgctaa attcgcaaaa gactttgcac attttgctaa tttcaccgta
    11281 ccgctctgtg acgtactata gtcgacaaac gtctcacctt gaggttaaaa aatgagcaac
    11341 atcctgatta tcaacggcgc gaaaaaattc gcccactcca atggtcaact gaacgacacc
    11401 ctgaccgaag tcgcggatgg cacactgcgc gacctcgggc atgatgtccg catcgttcgc
    11461 gccgacagcg actacgatgt caaagcggaa gtacaaaact ttctctgggc tgatgtggtg
    11521 atctggcaga tgccaggctg gtggatgggc gcgccgtgga cagtgaaaaa atacattgat
    11581 gatgtattca ccgaaggtca cgggactctg tatgccagcg atggtcgtac ccgcaaagat
    11641 ccgtcgaaaa aatacggctc cggcggcctg gtacagggca aaaaatatat gctttctctg
    11701 acctggaacg caccaatgga agccttcacc gaaaaagatc agttcttcca cggcgttggc
    11761 gttgacggcg tgtatctgcc gttccataaa gcaaaccaat tcctcggtat ggaaccgctg
    11821 ccgacattta tcgctaatga cgtgataaaa atgcctgatg ttccccgcta tactgaagaa
    11881 tatcgcaagc atcttgtgga aatttttggt taactagagc tcaggcttta gaaggagtta
    11941 accatgctta ccgtaatcgc agaaatccgt actcgtcctg gtcaacatca ccgtcaggcg
    12001 gtattggatc agtttgctaa aatcgttcca accgtactga aagaagaagg ttgccacggc
    12061 tatgcgccaa tggtggattg cgctgctggc gtgagtttcc agtctatggc accggattct
    12121 atcgtgatga tagagcagtg ggaaagcatc gcgcatcttg aagcgcatct gcaaaccccg
    12181 cacatgaagg cgtatagcga agccgtaaaa ggtgacgtgc tggagatgaa tattcgtatt
    12241 ctgcagccag ggatttaaca ttcctgaaaa gaggccggtg ctgtttgccg gccatttata
    12301 ttcactcttc cgccgtctgt aaccgtgctt taatctgttc gtaaaattca ttattaatca
    12361 gataaaatcg catgatgagt ccggtaatca ggttaagtat cgcgggaaag atcgtcatta
    12421 acaggcagat cccccacagc gcctgtggtg tttgttctac accaggttga taatccaaat
    12481 aggctaaagc gattccgata aagccccctg ccagccccat accgaacttt tggaaaaaga
    12541 gaatcccgcc aaatgccagg ccagaaacac gttgccccgt tttcatttca ccgtaatcca
    12601 cggactcagc aatggatgcc caataaatgg gaagctgaat atccgtcagg aaggtcacca
    12661 caatataaaa tgtaaaagct aacactatag aatttggttg cacgaaatac atcgcaccac
    12721 ccaccacgaa cgccagtaat tgggtataac gaaatatttt tattttgtcg tagagtcgag
    12781 taagccaggt acaagcgatc atagccagaa cagaggcgat cacgcctgaa gttaaaaacg
    12841 gtgaaattaa agcatcccca ccattcaggt aatattttgc ataatatgca gcgacagagt
    12901 tacgaataat cccgccaaac ataatgatcg caattacagc gcccaaaatg agtcattgat
    12961 catttttgat cagtaaccga aactgcgcgg ccataccgag actgggtttt ggatggtaga
    13021 tgcgctctct cacgctaaaa aaacaataca gacataaaca cgttgccaga attcccattg
    13081 cccccattgc tatttgataa cccagtttta cgttattctt cccaagatac attgcagata
    13141 aaggaacaat aattgttacg gcgaacatgg caatttttgt cataacaaaa cgatagccat
    13201 tggcgctcaa tcgctcttgt ggatcatccg tgataacgct gattaatgaa acataaggaa
    13261 tagcgattaa cgtataggtc aaggtaagta acacataggt agcccaggcc cataaaagct
    13321 taactgaata atcggtatca ggtgtggtga acatcaacca aatgcttatc ccaaacggta
    13381 ttgacataaa aagcaaccac ggccgatatt ttccccaacg tgtattcgtt ctgtctgtca
    13441 ttgttcccac aactggatca atcacagcat caaggatacg tacagaaaat aacagaatac
    13501 ctaagtcgac gggtttcaga ccaaatacat ccgtgtaaaa ataagtgatg atcatggcta
    13561 aagacatcat tactatcgca atagccatat caccggcacc aaaacctatc ttttcattta
    13621 ttgtcagttt catacacagc actcattgat cacgtgaaat tatattaaaa acaaatgtat
    13681 cggaactcat tcagcacata actttacgag tgagaataaa tgcattggta gataccacaa
    13741 caaaccactg agttaatatt gagcagcatc acataaaaac caatctcatt taacttaatg
    13801 atttttaata ataaatgtgg ttttttctgt tttatatcac agataacaaa gaagcatact
    13861 gcgcaataaa aataaaagaa tgtagagttg gtatcaacca cttgaaataa ggctttgtca
    13921 tgataagaat ttataaagcc agtaaaggtc agcttaagca tcgtgaaatt tttgatgaaa
    13981 taaagaaaaa gattgataac ggtgaatgga aagaaggcaa agctatacca acagaaagag
    14041 aacttgctga acatttttta agcagcagaa caacaattcg gaaagcaata gaaagtttga
    14101 aacaacgcgg atacctgcat agtgtccacg ggcaaggaac atttacatta ccggctcgca
    14161 gtcgggaaaa tcatttatta catagtttta ctgatgacat taaggctcgt ggcggcgtac
    14221 ctgcacagaa tattcttgaa ataggttata ttcctctaag tgatattatc agaaaaaatc
    14281 tcgaacttga tatccatgtc cataccgttt tctgtataaa acgcattcgc tatatgggaa
    14341 gtacgccgct gggtattcag acatcatggc ttgcgttaaa tgacggacaa gaaataactc
    14401 aggaagagct gctggcgacg ggttccctct atattttact ggaagaaaaa ctcgctatta
    14461 aacctttaga agcaacggaa attatctctg cccgacttcc ctcgcctctt gagcgtcaat
    14521 tactggaact gactgccgat gacgtcgtat taagttgtac ccgcgtcacg ttatccgtgg
    14581 aacgcaaacc aatggagtat gtagagatgg tttatcccgc aagtcggtac tcctacaaag
    14641 cacgaattac aaaagacagt tttagcgtgt aacagagaga atattgtgaa caaaatagaa
    14701 caagccgtaa tttataacaa aatattaggt tctttagcct gcgcgggtat gggcgatgca
    14761 ttaggcgcag cgactgaact ctacagcatt gatgaaataa aagcgcaatg gggaggtttt
    14821 ttaaatgcct ttgtttctcc tcctgccgat acctttgctg gctcattaaa tggtattgcg
    14881 ggacttatta ccgacgattc cagccaaatg tatgtatttt cagaagggtt agttgaggct
    14941 ggatttgata acttcaccaa taatgactgg ttagcttgtc ttctgcgttg ggccgatatg
    15001 cagccctacg caaattataa aggccccaca acagaacaaa tcgtcaaggc actaaaagaa
    15061 ggtagaccaa caaacaccat tgggcgtatt ggaacatctt ctcgtcaggc acccaatgtc
    15121 ggcaccacga acggcgcagg aatgcgtgta gccccggcag gattgatctg gccaggcaag
    15181 aaagaaaaag cgtgtcacct ggcgctaata acttgcctac cttcgcatga caccaatatt
    15241 gcgattgcca gcgcttgcgc catcgctgcg gcgacatcac aagctatgtt accagaagcc
    15301 tcgctcacat cattactgga cgccgcaatc tggggagcga attacggcga acgcctggcc
    15361 aaacaatatg cacgatgtgt cgcagggcca tctatcgcca tgcgcatcca acttgcagcg
    15421 gacatcgcca gacgagccaa tgacctggag tcttgtctgc gagaaatgga aggtttggtt
    15481 ggtaactctg ttgccgccca tgaatccatt cctgcggcaa tcggtctatt gctgtactgc
    15541 aaaggagaac cctgggaaac cattcatgcc tgcgccaata ttggcaatga caccgatagc
    15601 atcgctacga tggcaggcgc aattgcaggg gcatggcgcg gttttgatgc gctccctgaa
    15661 gataaatatg cctttttccg cgccgtaaat aataaagatt tcgatattga agcgatcgca
    15721 tcgggtctga cattgcttgc attacaggca caggagaaat aatgaacatg ccagcccgta
    15781 ctcctgttat gccattagcg caaactcgtg aggaaataaa tgctacggcg gcatcgttac
    15841 gccatttggc agctatctgg ggtgaatgtt tttctacttt tcagctcgat aaacgtgctg
    15901 ttgaacgcgt ctgtatagtg ggatcgggcg actcctggac ggtggcacta tgtgccgccg
    15961 cctggctggg gaaatacaca catctgtttt gttacgcatt gcaaacgtgg gattttcttc
    16021 agactgattt aacccgttat cagaaagaaa cgttaatcat tattctcagt gcaagtggca
    16081 gaccatcaat gaccgttgac gccctctgcc acgcagtatg ctcaaacgcg caagtgctgg
    16141 gtgtgacgaa ttgccctgga acaccatttt gcgcaataac agaaaacatg ctctatacat
    16201 gggcgaataa gcagggtatt ccaacgcaaa gtagtagcgt cacactttat gccctcctgc
    16261 gcttagcgca aaaattatgt ccaggcttaa caccactgca gattgaagag gatttggaag
    16321 ggaaatttag ccagattaat cagcactggc agcaaaagaa gcgccatttt taccaacaaa
    16381 aagagataac gtttcttggg agtggcttaa gttggggact ggcaatatcg gggagcaatt
    16441 tactttcttg cggcccacag attcgtgcga cagcccttcc actggaagaa ttttatcatt
    16501 cattacgctt acatcaggct ggtgtcgggc agcattatat tttactacca gccacatcct
    16561 gcgagtcccc attttacctt gcaacccaaa aagcaattgt tgaacaggga gcgacagcag
    16621 aattaatcag ttttatacct gacgccagtg agtccaacaa tttatttctg gtcatgcagt
    16681 ggttatatga aatgtgctgg catttaagtt gtgattatgt cgacgcaggc gggcaacgcg
    16741 ttagccatag ggagaaataa gactatgaag aaatttgatg tcgccgcttt aggcagtgga
    16801 aatatagata tttttttatc tatcccgtca ctccccactc gtggcggtaa agttgtcggg
    16861 acacgtctgg gtgaacaagc tggcggcaca gtcgccaaca gtgcctgtgc gatgggacaa
    16921 ctgggtctca atgtggtatc tgtttcatgt gtgggcaatg atcattcagc ctcgatcatt
    16981 cttgatggat ttaaaaaata tcatgtaaat tgtgatttcg ttcaggtaat tccggaattg
    17041 attgccaata ccgcgattat ctttattgat gaattaggtg aaaaaaagtt ggtttactcg
    17101 cctggtagtg atcatgagtg ggataaagaa aaagccctgc aggcaattgc gcaaagccgt
    17161 tatttttata cgatgccagc agatatcgag aaatttcgta tgctggccga atattcccgt
    17221 agccaaatga caaaagttgt cgttgatatt gagcctcata tcattgcaac acctgaacac
    17281 cttgccagaa ttttacacct tgctgatatt gcgatattta attatgacgg ttttattcgt
    17341 ggctatgcaa cagagccaga ttttacactt ctgcatgata tacaagacga gtatcaactt
    17401 gacgctgtcg tggttacgct ggatgttagg ggagtaattg cagtcaaagg caatgagcaa
    17461 gtaaaaatag gtagttatac aattcccgtt attgatacca caggcgctgg agatactttt
    17521 aatggtgcat ttgtttatag cttaataaaa aacatgccct taattgaagc actaaaattt
    17581 gcttctgcca cagcagcaat aaatattact gccttaggtg ccagaggtca tctgccgaca
    17641 ccatcggagg ttaatctttt tctttcacaa cacgactaaa atcatatata tacaaaggta
    17701 aatatcatga aaataagata tgccgctatt tttctggcat taccgctgtt atgcacaaca
    17761 gcgcaggcaa acagttttct tcattatgct ggggagttta acttccggac gggagaaaag
    17821 cagaacaatg tcacaatcgc ggggctatca gccatcacat ttgatgcaaa aaaaaatatt
    17881 ttctatgcta ttaacgatag cagaaacaat aataaagaag gagatgccag tctttataca
    17941 ctaaaaatac aggtatcatc caggggcatt gagcaagtga attttctcaa ccagcgtcca
    18001 ctgcttgatg caaaacagca gcctttcgtg accaatacgg tggatgccga aggtctggcc
    18061 ctgacacaca atggtaaatc attactctgg agttcggagt taggtgctcc actgcgtctg
    18121 agcactctcg atggcgtaat ggagaaagat tttacgtctt tatttcctgc tcgctttaat
    18181 attagttctg acaaagagag cagcaatggt attcgcagcg gcaacgcctg ggagggcctg
    18241 acagttacac cagatggaaa atcacttttt atcgccgttg aaagcagcct taaacaggat
    18301 gggcctattg caagtccaat aaattcaggc acagcacgct tactgcaatt ttctatagat
    18361 gcagacggtc gaccttcaaa gcaattgcat gaatatctct atattactga tcctgttccg
    18421 caagtgagta agttcggtat taatgataat ggcgtatccg aggtcctggc gctgaatgat
    18481 caccaattgt tggtcatcga acgttccggc agaaacgtca gtgctgggtt caatgactgg
    18541 gattactctg tcagagttta tatggttgat ttaactgctg ccagcgatat caagaatatt
    18601 gacagtctgc aagattggtc caataaatcc acgttacaac ccgtcagtaa aaaattatta
    18661 attgattttg ctgattacac ttcctctgcg gattgtattg aaggggtaac cttcggtcct
    18721 ctgattgatg ggcatacatc gttaatattt gtatctgata ataatttcca accccatcaa
    18781 cagacgaaat tttatctatt cattgataag gaaaacaaac ttaaaattta aagtcaagct
    18841 atagatacaa aaacattcta atacctggaa tcatcagata gtgtattcga ccaccataaa
    18901 tatattttat tctgaaaggg aatattatga attcttatct taaaatatca cttgcaggaa
    18961 taacaatgtt atcctgcctg tcacctacac tagtctgtgc agaacagccg catggattta
    19021 ttgattatcg tcatgagtat cttgatgata ctcgcaccca cgcggaccgc gttgaatttg
    19081 gtacattttt tagtaatggt atcggtttga tgggagaatt acgctataac accgatgaag
    19141 gtgataaaga taagtgggac ccctcacaat ttggcaataa cggaacagga ttatctgttg
    19201 tttaccgctt taagccgctg gatgacaaaa aattctggtt agaacccatg ttctggcttg
    19261 ataccacgca atattggtca acttacgaat atggtctttc tgcgggttat gacttcagca
    19321 aagaatggaa agtttcgggt cggtttcgtt atgacatgga taaagccacc gataaatcca
    19381 aaggttatgg caacgatgat cgtaataacc gccgttacga tgtgtggatc gactatcgac
    19441 cacaaaaaac caacttccaa tatcagttaa acctggtcta ttacaataat ggttatttaa
    19501 cctggaatga tggccataaa aactataccg catcatttaa agtagggtac aaaattggat
    19561 cgtggatacc ctatatgagt attgcggatt ataaaggtgt ggataaaaca tcaagtaatc
    19621 gccaaatccg ctggcgctgg ggtctgactt atactttcta atctggaata aaaaatgaat
    19681 aataaattta aaatcattct ctggatgatg ctctctgctc tgcctgcttt atcttacgca
    19741 caggtaaaaa atatagttac tggcgaatat agtgcggtca gtaaaattat caccgaaaag
    19801 gaaactctct atccatccga taagatatta ttggtttttg acattgataa tacgttatta
    19861 acgagtggca cacgcattgg cggtgatatc tggtatcaat ggcaaaccga taaattgcct
    19921 ttaaagccgg atgactcgca aaaagttcct tgcctgtatg aaaatacaat cagtatgctt
    19981 tacgaactta gccctatgca gcttaccgag ccacagttac cgacaatgtt aaaacagtgg
    20041 caacaaaagc ataccgcttt tgctctgaca tcccgtgcac cggatactct ctttccgaca
    20101 ttgcgggaat taaagcgaaa cggcattgat ttttcgtctt ccgcactcag gaagaaagag
    20161 gatactttgc caccctttga aaaagggaaa ctcaaacggt catggctgta cagccagggg
    20221 gtattttttt cttccgggca agataaaggc gtaattctcg attatatgct ggataaaatg
    20281 gggaacaaat atgacgcgat tgtttttatc gatgacgggc aagcaaacat tcatgcgatg
    20341 acgaagatgt tctctaagga taaatggttc tctacggatt ttaccattat tcactatacc
    20401 agagtggagg atgagcttat taaacaacaa ggtgcagtaa taacaactgc acaagcagaa
    20461 aaaatggcta cggactggaa aactctggct ggttcgctgg caacactatt tccagatcgt
    20521 aacaaactgt gcccaataaa ataatcaacg acaacccggc aaaacacatg tttgccgggt
    20581 taagattatc agacctcaat ctccgccatg tcgcctttct cttgcaacca gttgcggcga
    20641 tcttccgagc gtttcttcgc cagtagcatg tccatcatcg cgtcagtacg ctgatcgtct
    20701 tcatcatcga tagtcaactg caccagacgg cgagtgttcg gatcaagcgt ggtttcacgc
    20761 aattgcatcg ggttcatttc ccccagacct ttaaaacgct ggacgttcgg cttgcctttc
    20821 ttgcgtttta attgctcaag tacgccctct ttctcttctt ccgtcagcgc gtaataaacc
    20881 tctttcccga gatcaatacg gtagagcggt ggcagtgcga cgtaaacgtg accgtgtttc
    20941 accaacgcgc ggaagtgttt tacgaacaaa gcgcagagta gcgtggcaat gtgcagacca
    21001 tcagagtccg catccgcgag gatacagatt ttgccataac gaagctggct cagatcgtcg
    21061 ctgtcaggat cgataccgat cgctaccgaa atatcatgca cttcctgcga ggccagcact
    21121 tcgtcggaag aaacttccca ggtgttaagg atcttacctt tcagcggcat gatcgcctga
    21181 tattcacgat cgcgcgcctg cttggcagat ccgcctgcgg agtcaccttc cacgaggaac
    21241 agttcggtac ggttaaggtc ctgcgcggta caatcagcca gtttgccagg cagcgccggg
    21301 ccgctggtca gctttttacg caccactttt ttagccgcac gcatacgacg ctgggcgctg
    21361 gaaatcgcca tctctgccag caactccgcc gcctgaacgt tctggttcag ccacaggata
    21421 aaggcatctt tcaccacgcc agaaacgaat gccgcgcatt gacgcgaaga gagacgctct
    21481 ttcgtctgcc cggcaaactg cggatcctgc attttcaccg acagaacata ggcgcagcga
    21541 tcccagatat cttccgccga cagcttcacg ccgcgcggca gaatgttgcg atattcacag
    21601 aactcacgca tcgcgtccaa caggccctga cgcaggccat taacatgggt accgccctgc
    21661 atcgttggga tcaggttgac gtagctttcg gtcagcagtt caccgccttc cggcagccac
    21721 agtagcgccc agtccacagc ttcagtatcg ccagcgaaat taccgataaa cggtttttct
    21781 ggcagcgtcg gtaaaccgtt taccgcttcc gccaggtaat cattcagacc gtcctgatag
    21841 caccagcgct gttcggtgtt gttgatctca tctttaaagg taatctcaac gccggggcac
    21901 agtaccgctt tggctttcag cacatgcgtc aggcgtgaaa cagaaaatcg cgggctgtca
    21961 aagaaggttt catccggcca gaagtgcacg ctggtaccgg tattgcgttt accgcaagtg
    22021 ccgacaacct gtaaatcctg caccttttcg ccattttcaa aggcgatgtt ataaacctga
    22081 ccatcgcggc gcacgttaac ttcaacgcgc ttcgacaggg cgttaaccac cgaaatcccc
    22141 acgccatgca ggccgccaga gaactggtaa tttttgttag agaatttacc gcctgcatgc
    22201 agacggcaaa gaatcaattc aaccgccggt acaccctctt ccgggtgaat atccaccggc
    22261 atcccgcgcc catcgtcaat aacttctaac gactggtcag catgtaagat tacatccacg
    22321 cgttttgcgt gacccgccag tgcttcatcg acactgttat caataacttc ttgccccaaa
    22381 tggttagggc gagtggtatc ggtatacatc cccggacggc ggcgaaccgg ctcaagcccg
    22441 gtgagtacct caatggcatc agcgttataa gtttgcgtca tggtttaagt tagtaattcg
    22501 agttgatcgt cagagatggt gcagaccaag aaaatcgacg atcgggttga aataatcttc
    22561 gaagcccgtg aatgcgtggt tgccgccttc tatgacagtc tggcggcagg aagcgtagta
    22621 cgccaccgcc tggcggtaat ccagcacttc atctcccgtc tgttgcagca gccagatcaa
    22681 atccggcgct tccagcgggt caatctgcat gactttgaga tcgtaaatat ggcgtgactc
    22741 tagcacatat tgctgcccgg tgtaggggtt ctcgttctga ccgagatagt ccgtcagcag
    22801 ttcaaacggg cgcaccgccg ggtttaccac cactgcgggc agcataaaac attgtgacaa
    22861 ccaggtggcg taatatcccc ccagtgacga accgacaata cccagcgaat cgccgccatg
    22921 ttccaggaca atggattcca gcagctctgc cgcgtcggaa ggatacggcg gcaactgcgg
    22981 aatgatcatc tcaacgtcag ggtgatgttc cgccagccag ttttttaaca agctcgcttt
    23041 tgcagagcgc ggcgagctgt tgaaaccgtg taaataaaga agcgtagaca tcagtagcct
    23101 tctgaagcgg tatcaggttg gaaacgtgtg tccgccaggc gatgcacctc ggtggtcagc
    23161 gtgccatcag catgtaactc gagagtacgc cagccgggcg cgatagtatc cagcgtaaag
    23221 ttggaacagt gcggcttaaa ctgcacacag gtcgacggcg ttgccagcag gcggcgaccg
    23281 ttccagtcga gatccagctc ctgatgaata tgaccgcaca gcaagtattt gacgtgcgga
    23341 aacttcgcca gcacggtatc cagttcgccc gcgttacgca gactgtgttg atcgagccaa
    23401 ctacaacccg caggtagcgg atgatgatgc agcagcagca acgtatggcg ttctggcgca
    23461 tcggccagtt tacgttccag ccactcaagc tgaaactcgc tcagctcacc gtgcggcacg
    23521 ccaaacacct ggctatccag caacaggatt tgccattgct caccaataaa cacccgcttc
    23581 gccggggaga tacccgcatc ctgtaacgcg ctgtacatcg cgggctggaa atcgtggttg
    23641 ccaggcagcc agacgcaggg cgcacgaaaa cttgcgatgc cttcagcgaa atgctgatag
    23701 gccgcagagg attgatcctg cgctaaatca cctgtcgcga caatcaggtc gaattcgtgc
    23761 tggtgtggac gaatcgcctc cagcaccgcc tggtagcttt cccaggtatt tacccctaac
    23821 agggcttcgt gcttttgtgc aaacaggtga gtatcggtaa tttgtaaaat cctgactctg
    23881 gcctcaccag ccagaggaag ggttaacagg ctttccaaat ggtgtcctta ggtttcacga
    23941 cgctaataaa ccggaatcgc catcgctcca tgtgctaaac agtatcgcaa ccagtccgct
    24001 aaaaactgat taatttgatg cttttcgtcg cgttgatgca actttttatt aggataatca
    24061 taccgcgctt tgaagcgaaa aatctgctgg cttgaacaca cttcagccac catcgcgtca
    24121 tgatacagac gcaccgtcat tgacggaagg ctccagtaac tgatcgcggg cgcagtctgt
    24181 tctattgtca ccagggtagt gtatcgggtc gattccacaa tcgtcagccg atattgtgcg
    24241 tttgccacct gatagcttac agtttcgccg ggtgcgtcat tgcgcggtaa caaacggcgc
    24301 aattgtgaaa agttcatctc gcacaggcgc atcatttcag gaaagtcagg tgtgtaacgc
    24361 ttcatttatg cccactcatt ttttaatgct tgatgatgca gctgcagcca ttgcaaagcg
    24421 atgaccgacg ctgcgttgtc gattttcccc tcttctaccc actggtatgc ctgttcccgg
    24481 cttaccacat gaacgcgaat atcttcgttt tcatcagcca gaccatgaat accgcttgcg
    24541 gtcgtggcgt ccacttcgcc caccataatt gacgaacgct cactggtgcc tcccgggctt
    24601 gccaggaaac ttaacaccgg tttggtccgt ttgactatca gtcccgcctc ttcaatcgct
    24661 tcgcgacggg caacatcttc cacactttca ccctcttcaa tcatcccggc aaccatctcc
    24721 agtagccagg gggtttcact ggtgtcgtat gcggcaatcc gaatctgctc aatcagcaca
    24781 acttcatcac gcactgggtc aaagggtagc aagactgcgg cgtgaccgcg ctcaaaaatt
    24841 tcccgccgta cctcatgact catttgcccg ttgaataaac gatgacgaaa tctataaaga
    24901 tctaatgaaa aaaagccgcg ataaagtgtt tctcgtgcaa taatttctac atcgtttttg
    24961 ccaagtgtaa cgggcaggtt gtctggctta agcattgtta atgtcctggc actaatagtg
    25021 aattaaatgt gaatttcagc gacgtttgtc tgccgtttga gcagtcatgt gttaaattga
    25081 ggcacattaa cgccctatgg cacgtaacgc caaccttttg cggtagcggc ttctgctaga
    25141 atccgcaata attttacagt ttgatcgcgc taaatactgc ttcaccacaa ggaatgcaaa
    25201 tgaagaaatt gctccccatt cttatcggcc tgagcctttc tgggttcagt tcgttgagcc
    25261 aggccgagaa cctgatgcaa gtttatcagc aagcacgcct tagtaacccg gaattgcgta
    25321 agtctgccgc cgatcgtgat gctgcctttg aaaaaattaa tgaagcgcgc agtccattac
    25381 tgccacagct aggtttaggt gcagattaca cctatagcaa cggctaccgc gacgcgaacg
    25441 gcatcaactc taacgcgacc agtgcgtcct tgcagttaac tcaatccatt tttgatatgt
    25501 cgaaatggcg tgcgttaacg ctgcaggaaa aagcagcagg gattcaggac gtcacgtatc
    25561 agaccgatca gcaaaccttg atcctcaaca ccgcgaccgc ttatttcaac gtgttgaatg
    25621 ctattgacgt tctttcctat acacaggcac aaaaagaagc gatctaccgt caattagatc
    25681 aaaccaccca acgttttaac gtgggcctgg tagcgatcac cgacgtgcag aacgcccgcg
    25741 cacagtacga taccgtgctg gcgaacgaag tgaccgcacg taataacctt gataacgcgg
    25801 tagagcagct gcgccagatc accggtaact actatccgga actggctgcg ctgaatgtcg
    25861 aaaactttaa aaccgacaaa ccacagccgg ttaacgcgct gctgaaagaa gccgaaaaac
    25921 gcaacctgtc gctgttacag gcacgcttga gccaggacct ggcgcgcgag caaattcgcc
    25981 aggcgcagga tggtcactta ccgactctgg atttaacggc ttctaccggg atttctgaca
    26041 cctcttatag cggttcgaaa acccgtggtg ccgctggtac ccagtatgac gatagcaata
    26101 tgggccagaa caaagttggc ctgagcttct cgctgccgat ttatcagggc ggaatggtta
    26161 actcgcaggt gaaacaggca cagtacaact ttgtcggtgc cagcgagcaa ctggaaagtg
    26221 cccatcgtag cgtcgtgcag accgtgcgtt cctccttcaa caacattaat gcatctatca
    26281 gtagcattaa cgcctacaaa caagccgtag tttccgctca aagctcatta gacgcgatgg
    26341 aagcgggcta ctcggtcggt acgcgtacca ttgttgatgt gttggatgcg accaccacgt
    26401 tgtacaacgc caagcaagag ctggcgaatg cgcgttataa ctacctgatt aatcagctga
    26461 atattaagtc agccctgggt acgttgaacg agcaggatct gctggcactg aacaatgcgc
    26521 tgagcaaacc ggtttccact aatccggaaa acgttgcccc gcaaacgccg gaacagaatg
    26581 ctattgctga tggttatgcg cctgatagcc cggcccccgt cgttcagcaa acatccgcac
    26641 gcactaccac cagtaacggt cataaccctt tccgtaactg atgacgacga cggggcttcg
    26701 gccccgtctg aacgtaaggc aacgtaaaga tacgggttat ctgccgcatt cttccccctt
    26761 ctcgcttcaa tttcgaccag ccatcctcta ttctgatggg tatttaccac tggtcccgga
    26821 agacaaaaat gaaacggaca aaatccatac gccacgcatc gttccgcaaa aactggagcg
    26881 cacgccatct gacaccagtc gctctcgcgg ttgccactgt ttttatgctg gctggctgtg
    26941 aaaagagtga tgaaacagtg tctctctatc aaaatgctga cgactgttca gctgcaaacc
    27001 caggcaaaag cgccgaatgt accaccgcgt acaacaatgc gctgaaagaa gccgaacgta
    27061 ctgcgccgaa atacgccacc cgtgaagact gtgttgctga atttggtgaa ggtcagtgcc
    27121 agcaggcacc agcccaggct ggcatggcac cagaaaacca ggcgcaggcc cagcaatcca
    27181 gcgggagttt ctggatgccg ctgatggccg gttacatgat ggggcgtctg atgggcggcg
    27241 gcgcgggatt tgcacaacag ccgctgttct cctcgaaaaa cccagccagt ccggcttacg
    27301 gtaaatatac cgacgcgacg ggtaaaaact atggcgcagc ccagccaggc cgcaccatga
    27361 ccgtaccgaa gacggcaatg gcaccaaaac cggcgaccac cactaccgtt acccgtggcg
    27421 gttttggtga atctgttgcc aaacaaagca ctatgcagcg tagtgcaacc ggtacctctt
    27481 ctcgttcaat gggtggctga taccgatgga aagagtcagt attaccgagc gcccggactg
    27541 gcgtgagaaa gcccacgaat acggtttcaa ttttcacacc atgtacggcg agccgtactg
    27601 gtgtgaagat gcttactaca agttgaccct cgcccaggtt gaaaagctgg aagaagtcac
    27661 cgccgaactg caccagatgt gcctgaaagt ggtggaaaaa gtgatcgcca gcgatgagct
    27721 gatgaccaaa ttccgcattc caaaacacac ctggagtttt gtgcgccagt catggctgac
    27781 gcaccagcca tcgctttatt cgcgtcttga tctggcgtgg gatggcactg gtgaacctaa
    27841 acttctggaa aataacgccg atacgccaac gtcactatac gaggcggcgt tctttcagtg
    27901 gatctggctg gaagatcagc ttaacgccgg taacttgccg gagggcagcg accagtttaa
    27961 cagtctgcaa gaaaaactga tcgatcgctt cgttgagctg cgtgaacagt atggcttcca
    28021 gttgctgcat ctcacctgct gtcgcgacac ggtggaagat cgcggaacca ttcagtattt
    28081 gcaggactgc gcaacggaag ctgaaattgc tactgagttc ctctacatcg atgatatcgg
    28141 gttaggtgaa aaaggtcagt tcacggattt acaggatcag gtaatttcca acctgttcaa
    28201 gctgtatccg tgggagttta tgctgcgtga gatgttctcc accaagctgg aggatgcagg
    28261 cgtacgctgg ctggaaccgg cgtggaagag cattatctcc aacaaggcac ttctaccgct
    28321 gttgtgggag atgttcccga atcacccgaa cctgctgccc gcttattttg cggaagatga
    28381 tcatccgcaa atggaaaaat atgtggttaa gccgatcttc tcccgtgaag gcgcaaacgt
    28441 gtcgatcatt gagaacggca aaaccattga agcagcggaa ggtccgtatg gcgaagaagg
    28501 aatgattgtt cagcaattcc acccgttacc gaaattcggc gacagctata tgctgattgg
    28561 tagctggctg gtgaacgatc aacctgccgg aattggcatt cgtgaagacc gtgcattgat
    28621 cacccaggat atgtctcggt tttatccaca tatttttgtt gaataatcaa tgataccgga
    28681 tggcattcgc catccggtaa ttgttaacct acctgcaccg acagcatact caggctgccc
    28741 atttctatac cctcaaccgg aatggtaatt ggctcctgcc catcccacgc acctaacaca
    28801 tacaacaacg gcagataatg ctctggcgtt gggttcgata acgtgccacc ttcatggtcg
    28861 aggtaattca ccagaggatg ttgttccact ggcccttgcc acgtcagatt cgctttcaca
    28921 tactcattaa acgacgtcgc ccacggatac ggtgaactat caccgtgcca cttcactgtg
    28981 cgcaggttat gcaccacgtt gccactggcg accaacatta ttccttcatc tcgcagcgct
    29041 gccagtttgc gccccatttc gaaatgccag gcggcaggtt tgctactgtc gatactcaac
    29101 tgcaccatcg ggatatcggc gtcaggatac atcttaatca gcacgcccca ggaaccgtgg
    29161 tcaaagcccc aggcttcttt atccagcgtc accgggaccg gcgctaacag ctcaaccaga
    29221 tgctgtgcca gcacaggcga acccggagca ggataatgcg tatcgtacag cgcctgcggg
    29281 aagccaccaa agtcatgaat cgtgggcggc gtctccatcg cggtcactcc tgttccacgg
    29341 gtaaaccagt gagccgaaac caccacaatc gcttgcgggc gtggcaatgt catccccaac
    29401 ttctgccagc tgcgggtata caaattatct tccagcacgt tcatcggact accgtgacct
    29461 aaaaacaatg ctggcatacg tgttgaagac atgatgatat ccttaactaa aggtgtcatt
    29521 ttgatatcct cacaatacgc ttgttcggcg gagtaagaac ccggataaca atgatgatga
    29581 tcatcagtta ttttgacgat ctgcctgaag gtgaagattt ataaggagtt gtcgatgtca
    29641 gtacctctca ttctgaccat actggcgggg gcagccacgt ttattggcgc gtttctcggc
    29701 gttctcgggc aaaaaccctc gaaccgctta ctggcgtttt cgctaggatt tgcggcgggg
    29761 atcatgttgc tcatctcatt aatggaaatg cttcctgccg cactagcagc tgaaggaatg
    29821 tcgcctgtgt tgggttatgg aatgtttatc ttcggtctgc ttggctattt tggcctggac
    29881 cgcatgttgc cacatgcgca tccgcaggat ttaatgcaaa aatcggtgca gccgttgcca
    29941 aaatcgatca agcgcacagc cattctgctc actctcggca tcagtctgca taacttcccg
    30001 gaagggattg ccacctttgt cacggcgagc agcaacctgg agctgggatt tggcatcgca
    30061 ctggccgtcg cgttgcacaa tattcctgaa ggtctggcgg ttgcaggccc ggtttatgca
    30121 gcaacgggtt ctaaacgcac ggcaattctg tgggcgggga tttctggatt ggcagaaatt
    30181 cttggtggtg tgctggcgtg gttaatcctc ggtagcatga tttcccccgt ggtcatggcg
    30241 gcaatcatgg cggcggttgc aggaattatg gtggcgctct cggttgatga attaatgccg
    30301 ctcgccaaag agattgaccc taataataac cccagctatg gcgtactatg tggaatgtca
    30361 gtgatgggat tcagtttagt gctgctacaa acggcgggaa ttggttaaaa gcaacatcag
    30421 aagttggcca gcgcatggac aacttctgct agcttcacta aagccttatt ctaccttttc
    30481 ggctgtctct tctctcgtac tgtttaagta tttgttccgc gcttcaagca gtgcgtctct
    30541 gtcctggcga aacgcccgac aataacgttt caaatgcagg aaataaccca ccacaacaag
    30601 agcaatcaat acaatccagt accaggcgat aaacatcctt ttacccttaa tatttaacaa
    30661 tactaaacat ttagcgcata aatttcacat atcctttttc ggatatattc atatggtcgc
    30721 ataataacgg aacaattata tgattttgat cataatgaaa aagaatgcct gatattaatg
    30781 aaattttctc atagaaagag aagcggaatt aacatcttgc atagcagggc aaaataaaaa
    30841 ccgaagccag ctacaacttc ggtttcttga ttaaggcagt aattaagcag cggttttcag
    30901 ctggctttac gctcatgtgc ctgacggtat gccaccaggt cttcaatagt cacgagcgcc
    30961 atattgtgtt tattggcaaa ctcaatgcac tctggtgcac gcgccatcgt gccatcgtca
    31021 ttagtcaact cacacagcac accagccggt ttaaagcctg ccagcgtcat caaatcaata
    31081 gtcgcttcag tatgaccgcc acgcgtcagt acaccacctg cctgcgcgcg aagtgggaaa
    31141 acgtggcccg ggcgattcag atcagaaggt tttgcgccat cggcaatcgc tgcgcgaacg
    31201 gttgtaatac gatcagcggc agaaacaccg gtagtcacac cttcagccgc ttcaatggtc
    31261 acggtaaaac cggtgccata ggcgctggtg ttattttcta ccatcattgg cagatcaagt
    31321 tgtttacggc gatcttcagt gatgcacagg caaacaatac cgctaccgtg gcgaatggtc
    31381 agcgccatct ggtcaacggt catggtttct gccgggaaga tcatatcgcc ttcgttttca
    31441 cggtcttcat catcaagcac cattacaccg cgtccttcac gcagcgcagc cagtgcattt
    31501 tcaacacgtt cgaaaggcgt accaaaagag gaaagtagcg tctgattcat ggtaaaaaaa
    31561 cctcactaaa attatggtta ccagaatcag ggcagtctta ggagtggcgg catatagcca
    31621 aaataacgtg agcgggtcca tgcccgacag aatcgttact ctctcccatc cggactctaa
    31681 ccgtcggccc cggaattaca ccggatctgc tgtcctttga gttcgcaccc aaagcgctcg
    31741 cgggctttca actgagttga tttaccgccg gtggggaatt tcgccccgcc ctgagaataa
    31801 gcggattcac tataacgcta atgattagcg gcagcaacgc atagcttcac ataattctgg
    31861 tttatgactt acccttatcg cactacaatg gcactcaaca cctatcagta cagggaaact
    31921 acaatgattg acccgaaaaa aattgagcaa atcgctcgcc aggttcacga atcaatgcct
    31981 aaaggaatca gggagttcgg ggaagatgtg gagaaaaaaa tccgccaaac cctacaagct
    32041 cagctgacgc gcctcgacct tgtaagccgt gaagagttcg acgtccaaac gcaagtcctg
    32101 ttacggactc gtgagaaact ggcgctgctt gaacaaagaa tgagcgaact ggaaaatcgc
    32161 agtactgaga ttaaaaagca gccagatcca gagactctcc ccccaacgct gtaaacattt
    32221 gtctttaatg cactgtaaat tatgaattaa tcctctaatt tacagtgctt tctattacct
    32281 tatcgcttaa ttccattcag aatatcatca caaaaatgaa tgagtgcaat tgaaaatata
    32341 attgttatgt attttttaag ttttacttaa tccatattta gataaactaa tttcataaaa
    32401 cccgaaaagt aaaatataat atttgaatga ttagcaatcc ttaaataaca gttactaatc
    32461 attcattttc tacttatatt tatataaagg aatattatgt ctgcttttaa aaagtcactt
    32521 cttgttgctg gcgtagcaat gattctttcc aataacgtat ttgctgacga aggtcacggc
    32581 attgttaaat ttaaaggtga agttatctct gctccatgtt caattaaacc atgtgacgaa
    32641 gacttaactg taaatctggg tgaagtagca gataccgttc tgaaaagcga ccagaaatct
    32701 ttggctgagc ctttcactat tcacctgcaa gactgcatgt tgagccaggg tggaaccact
    32761 tattcaaaag cgaaggttac tttcaccaca gcgaatacta tgacgggtca gactgacctg
    32821 ctgaaaaaca ccaaagaaac tgaaatcggt ggtgccacag gcgttggcgt acgtattctg
    32881 gatagccaga gcggcgaagt gactttaggt actccggtag taattacatt caacaatact
    32941 aacagctatc aggaactgaa cttcaaagct cgtatggaat ccccaagcaa agatgccacc
    33001 ccgggcaacg tttacgctca ggctgactac aagattgctt acgagtaatc gcatttggcc
    33061 gggttcaccc ggccacatca ttaacggatt aatgataagt ggatcagatg tataaaaaat
    33121 taaaattaac cacaataagc gaattgatta aaaatattta ttgttcatta tccgttctca
    33181 tcattggttg tgcgtcagct tatgccgttg aattcaacaa agatttaatc gaagccgaag
    33241 atcgtgaaaa cgttaacctt tcccaatttg aaactgatgg ccaattaccc gtaggtaaat
    33301 actcattaaa cgctcttatt aataataaaa ggacgccaat ccatctggac ctgcaatggg
    33361 tattaattga taaccaaact gcagtttgtc tgactccaga gcaattaaca ttattaggct
    33421 ttactgatga gattattgaa gaggctcagc aaaacctgat cgatggttgt taccctatcg
    33481 aaaaagaaaa acaaattaca acttatctcg ataaagggaa aatgcaatta tccatatctg
    33541 cacctcaggc atggttaaaa tacaaagatg caaactggac gcctcctgaa ctttgggatc
    33601 atggtattgc tggggcattt cttgactaca atttatatgc ctctcattat gcaccacatc
    33661 agggcgataa ttcacaaaat ataagttcct atgggcaggc tggggttaat cttggggcct
    33721 ggcgcctgcg tactgattac cagtacgatc agtcatttaa caatggcaaa agccaggcga
    33781 acaacctgga ttttccgcgt atttatttgt ttcgcccaat cccagcaatt aatgcaaaac
    33841 taactatagg tcaatacgat actgaatcct ctattttcga ctctttccat ttttctggcg
    33901 tttcgttgaa aagcgatgag aatatgttgc cgcctgatct acgtggttac gcaccgcaaa
    33961 tcacgggtgt tgcacaaacg aatgcaaagg tcactgtctc acagaacaac cgtattattt
    34021 atcaagaaaa tgttcctcct ggcccatttg ctattactaa tttattcaat acattacagg
    34081 ggcaactgga cgtcaaggtt gaagaagagg acgggcaagt cacgcaatgg caagttgcat
    34141 ctaatagtat tccttatctg acgcgtaaag ggcagattcg ctacaccact gctatgggta
    34201 aaccgaccag cgttggtggt gattccttac aacaaccctt cttctggact ggtgaattct
    34261 catggggttg gctgaacaat gtatccctgt atggtggttc agttttaaca aaccgtgatt
    34321 atcaatctct ggctgccggc gttggcttta accttaactc attgggttca ttatcttttg
    34381 atgtcacacg atctgatgct cagttgcata atcaggataa agaaacgggt tatagctacc
    34441 gcgctaacta ttcaaaacgt tttgaatcta ccggtagcca gctcactttc gctggttacc
    34501 gtttctctga taaaaacttt gtgacaatga atgaatatat caatgacact aaccattaca
    34561 cgaattatca gaatgaaaaa gagagttata ttgtcacgtt taaccagtat cttgaatcat
    34621 taaggttaaa tacatacgta agtttggctc gtaatactta ctgggacgcc agcagtaatg
    34681 tgaattattc attatcactt agccgcgatt ttgatatcgg gccattaaaa aacgtctcca
    34741 cttctctaac atttagccga ataaactggg aagaagacaa ccaggatcaa ctgtacctaa
    34801 atatttcgat tccctgggga accagtagaa cattgagcta tggtatgcaa cgaaatcagg
    34861 ataacaagat ttcgcatact gcttcgtggt atgactcttc cgatcgaaat aattcctgga
    34921 gcgtttctgc ttcaggcgac aatgatgaat tcaaagatat gaaagcgtca ctacgcgcca
    34981 gttatcagca taataccgag aacggtcgtc tctatctctc cgggacatca cagcgagaca
    35041 gttattattc tctgaatgcc agttggaatg gttcattcac ggcgactcgt cacggagccg
    35101 ctttccatga ctatagtggt agtgctgact cgcgttttat gatcgacgca gatggtgcag
    35161 aagatattcc gttgaacaat aaacgcgcgg taactaatcg gtatggcatc ggagttattc
    35221 catcagtcag cagttacata acaacatcat taagcgttga tacccgaaat cttccagaaa
    35281 atgtggatat cgaaaactcg gttatcacca ccaccttaac cgaaggtgct attggctacg
    35341 ccaaacttga tacccgcaag ggctatcaaa tcatgggggt tattcgcctg gcagatggta
    35401 gtcacccacc actggggatt agcgtaaaag ataaaaccag ccataaagaa ttaggactgg
    35461 ttgctgatgg cggctttgta tacctcaacg gcattcagga tgacagcaaa cttactttac
    35521 gctggggtga taaatcctgt tttattcaac cacccaatag cagcaactta accaccggaa
    35581 cggttatttt accgtgtatt agccaaaatt aattcaggat gaaactaatg cgctacttaa
    35641 acacaaagaa tctcattgca gcaggagtat tactcgcctg catgagcagc attgcctggg
    35701 gtgccatcat cccggaccgt acccgtatca taatgaatga gtcagataaa ggggaagcat
    35761 taaaacttac taatcaaagc aaaaatctac cctatctggc gcaaacatgg atagaagata
    35821 cgaaaggcaa taaatcacgt gactttattg tcacggttcc ccctatggta cgtttaaatc
    35881 ccagtgagca aattcaaata agaatgataa gtcaggaaaa aattgctcaa ctgcctaatg
    35941 acagagaaac gctcttctat tttaacgtgc gagaaatacc gccaaaaaca gataaaaaaa
    36001 atgtgatgca agtcactatg cagcacgcat tgaaactatt ctggcgacca aaagctattg
    36061 aattagaaga tgatggggta atgtcttacg aaaaagttga aatcatccgt agaaatgatg
    36121 gttcgatccg ctttaacaat aagatgcctt atcacgttac gctgggttat atcggaacaa
    36181 acggaatcac aatgttgcca caaacgcaaa gtttaatggt aaccccttat agccatgcag
    36241 atacgcagtt taaaaatgtt ccgtcagctt ttcaggttgg ttatatcaat gattttggtg
    36301 gattaagttt ttatgaaata aactgcccga cggttaataa ctcttgtaat gtttccgtag
    36361 ccaaacgaga taaataatat gcgctactta ttaattgtaa ttatatttat tataggtttt
    36421 agtgcattac ctgtatgggc aatggactgc tatgctgaac atgaaggggg aaataccgta
    36481 gtcattggtt acgtaccaag aatttctatc cccagtaatg gtaaaaaagg tgataagatc
    36541 tggcaaagca gtgagtattt tatgaatgtc ttctgtaata atgcattacc tgccccatca
    36601 cctggagaag aatacccatc agcctggact aatataatga tgtttttacc aggcggtcag
    36661 gacttttata atcagaactc ctatattttt ggcgtaacat acaatggagt tgattatgat
    36721 tcgactgccc ctaatgctat cgcagcacca gaatgcattg atataaaagg agcgggtacc
    36781 tttaataatc actataaaaa ccccgctgtc tgtagtggag gtcctgagcc tcaattatca
    36841 gtaacctttc cagctcgcgt acaactctat attaaattag caaaaaatgc caatagagtt
    36901 aataaaaaca tggtgttacc tgatgaatat atcgctctag agtttaaagg tatgagtggc
    36961 gcaggagcta tagaagtaga caaaaatttg accttcagga ttcgtggatt aaataatatt
    37021 catgtccttg actgctttgt taatgtcgca ctggaaccag ctgatggcgt tgttgacttt
    37081 ggtaaaataa attcccgaac aattaaaaat accagcgtta gtgagacgtt tagtgtagtc
    37141 atgacaaaag atcctggtgc ggcctgtact gagcaattca atattttagg gagctttttc
    37201 actacggata ttttgagtga ttatagccat ctggatatgg gtaatggact gctattgaag
    37261 atatttcata aagatggaac agcaacggaa tttaaccgct tctcacaatt tgcttctttt
    37321 tcatcgtcta gtgcaccttc ggtcaccgca ccattcaggg cagaacttag tgcgaatccg
    37381 gcagagactg ttgttgaggg accgtttagt aaagacgtca tcctcaaaat cacctataac
    37441 tagcatctaa tacaaacact aaaacgggcc atcaggcccg ttatatcagt gctctaactc
    37501 cagctctctt gcctgcatca tctgttgaca ataccaggca taacgtgcgc aaaaccaggt
    37561 tctatgttct tcatccatgt taccagtaac ggcaagaata tcgaccgggc gaccttctgc
    37621 gtgcattctg gcaaaactac gcgccaggaa atcgaaatta tttaaagaat taagactatg
    37681 atccataagc acatcctccg gttatcgcat accttttaag gccatatgct ttttctgatt
    37741 tcagtattcc ccaatcagtg tagtgccttt aaacataacg tgcgtaaata tattgtcccc
    37801 gatcacactt tttagtgaaa aataaaaaac catattaaaa aactttctct tccagaactt
    37861 ctctaacatg tgttattatt aacaaagata aatgtaagca aggaaactat cccaaagtaa
    37921 atattgcgtc actaaatgga cattggagtg acatatgatt ttattcgccg actataatac
    37981 cccttatctt ttcgctattt cctttgtact gttgatcggc cttttagaaa ttttagcgct
    38041 tatctgcggt cacatgctct ccggtgctct tgatgcacat cttgaccatt atgattccat
    38101 aaccacaggt catattagcc aggcacttca ttatctcaat atcggaagat tgccagctct
    38161 cgtcgttctc tgcttgctgg ctggtttttt tggtcttatt ggcatcctgt tgcagcatgc
    38221 ctgcatcatg gtctggcaat caccactgtc gaacctgttc gtggttcctg tcagtttact
    38281 gtttacgatt attgcggtgc attataccgg taaaatagtt gcgccctgga ttccacgcga
    38341 ccatagttca gctattacag aagaagaata tattggtagc atggcattaa ttaccggtca
    38401 tcaagccact tcaggtaatc cctgcgaagg aaaactcacc gatcaatttg gccaaataca
    38461 ttatttacta ctggagcctg aagaaggaaa atttttcact aaaggagaca aagtgttaat
    38521 tatttgccga ctttctgcaa cgcgatattt ggcggaaaac aatccctggc cacaaatatt
    38581 gtaatttatt aatcaaaagg aaatttaata atggatgata ttgttaattc tgtgccctcc
    38641 tggatgttta ccgcgattat tgccgtatgc attctgttta ttattggaat tattttcgcc
    38701 aggctctatc gtcgcgcttc ggcagagcaa gcttttgttc gtactggttt aggtgggcaa
    38761 aaagtggtga tgagcggtgg cgcaatcgtg atgccgatct ttcatgaaat aatccccatc
    38821 aatatgaata ctctgaagct ggaagtcagc cgctcaacca ttgatagcct gattacgaaa
    38881 gatcgtatgc gtgtcgatgt agtagtcgct ttctttgtac gggtaaaacc gtctgtagaa
    38941 ggcatcgcca ctgcggcgca gacgttgggg caacgcacgc tatcaccaga agacttacgt
    39001 atgttggttg aagataaatt tgtcgatgcc ctccgtgcaa cagctgcaca aatgaccatg
    39061 catgagttac aggatacccg cgagaacttc gtgcagggag tacaaaatac tgtcgcagaa
    39121 gacctgtcga aaaacggtct tgagctggaa agcgtttcac ttaccaactt taaccagacc
    39181 tcgaaagaac atttcaatcc gaacaatgcc tttgacgccg aaggtttaac caaactgact
    39241 caggagacgg agcgccgtcg tcgcgaacgt aacgaagttg aacaggatgt agaagttgcg
    39301 gtgcgtgaga aaaaccgtga tgcactttcg cgcaagttgg agattgaaca acaagaagcg
    39361 tttatgacgc ttgagcagga gcagcaggtt aaaacccgta ctgccgaaca gaatgcacgt
    39421 attgcggctt ttgaagctga acgtcgtcgt gaagcagagc agacgcgaat tctggctgaa
    39481 cgacaaattc aggaaacgga gatcgagcgc gagcaggccg tccgctcaag aaaggttgag
    39541 gctgaacgtg aagttcgtat taaagagatc gaacaacagc aggttaccga aatcgccaac
    39601 cagacgaaat cgatcgctat tgccgccaaa tcggaacaac agtcccaggc agaagcgcgt
    39661 gctaaccttg cactcgcaga agcagtaagt gcgcaacaaa acgtagaaac cactcgccag
    39721 accgcagaag ccgatcgtgc taaacaagtt gccctaatcg ctgccgcgca ggatgcagaa
    39781 accaaagcgg ttgaactgac cgtgcgggcg aaagcagaaa aagaagccgc agagatgcag
    39841 gcggcggcta tcgttgagtt agccgaagct acacgtaaaa agggtctggc ggaagcagaa
    39901 gcacaacgtg cgctgaacga tgctatcaac gtactttctg atgaacaaac cagccttaaa
    39961 ttcaaactgg ccttgttgca ggcgctacct gcggtaatag aaaaatccgt tgagccgatg
    40021 aaatctattg acggtatcaa gattattcag gtcgatggtc tgaatcgtgg cagcgctgcg
    40081 ggtgatgcaa acacgggtaa tgtggggggc ggaaacctgg cggaacaagc attatcagcc
    40141 gctctctctt accgcacaca ggcaccgctg attgactcct tgctcaatga aattggcgtt
    40201 tcaggcggct cactggcggc attgacttca cccttaacct caacaactcc cgtcgccgaa
    40261 aacgtagaat aaattttcag ctatgaacgt ccctggcaca aaaagccggg gattttttat
    40321 atctgcgttc cgctaaaaac tgcaaatgat caggtcgttg caacgttttg cgtgaccgct
    40381 cggggaaggc aaaactgcct ctgggaaagc gttgcgcgag gtccggcgct catcaacaat
    40441 cgggaagtca gcaaagggct gaaacgggaa agcccctccc gaggaagggg ccttacagaa
    40501 ggaaagggtt atgatgaagc tcgtcatcat actgattgtg ttgttaatcg taagtttcgc
    40561 agcttattaa cagccaatca gaggggggag aaatcctccc tcacccttgt tcctttactt
    40621 taggttgaaa aaaaatcagc gtcaagaagg tttcattaac acatatccct ggcccaaaaa
    40681 gccggggatt ttttatatct gcgttccgct aaaaggtgca aatgctcagg acgttgcagc
    40741 gttttgcgtg accgctcggg gaaggcaaaa ctgcttcagg gaaagcgttg cgcggggtcc
    40801 ggcgctcatc aacaatcggg gagtcagcaa ggggctgaaa cgggaaagcc cctcccgagg
    40861 aaggggcctt atagaaggaa agggttatga tgaagttcgt catcatactg gttgtgttgt
    40921 tactgttaag tttcccgact tactaacaac tgatcagagg ggggagaaat cctcccttac
    40981 ccttgttcct ttactttagg ttgaaaaaac aacagcgtca ataggcctgc ctgctacgaa
    41041 gcgagatctg tgaaccgctt tccggttagc cttttttatc ctgttggatc ttcttgatga
    41101 tgttagtggt cgagcaaccg tcttcgaagt tgagcaccag cacttcgccg ccgttggccc
    41161 agacttcttt a
//
LOCUS       DAMZET010000020        40986 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.020, whole genome shotgun sequence.
ACCESSION   DAMZET010000020 DAMZET010000000
VERSION     DAMZET010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.020"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(204..1190)
                     /gene="yeiR"
                     /locus_tag="POB34_001202"
     CDS             complement(204..1190)
                     /gene="yeiR"
                     /locus_tag="POB34_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HDI5918189.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(1308..2774)
                     /locus_tag="POB34_001203"
     CDS             complement(1308..2774)
                     /locus_tag="POB34_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDI5918190.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            complement(2997..3569)
                     /gene="yeiP"
                     /locus_tag="POB34_001204"
     CDS             complement(2997..3569)
                     /gene="yeiP"
                     /locus_tag="POB34_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HDI5918191.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            3724..3978
                     /locus_tag="POB34_001205"
     CDS             3724..3978
                     /locus_tag="POB34_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDI5918192.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(3975..5156)
                     /gene="setB"
                     /locus_tag="POB34_001206"
     CDS             complement(3975..5156)
                     /gene="setB"
                     /locus_tag="POB34_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HDI5918193.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            5524..6654
                     /gene="fruB"
                     /locus_tag="POB34_001207"
     CDS             5524..6654
                     /gene="fruB"
                     /locus_tag="POB34_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HDI5918194.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGGDAEQALKAIGDAIAAGLGEGA"
     gene            6654..7592
                     /gene="fruK"
                     /locus_tag="POB34_001208"
     CDS             6654..7592
                     /gene="fruK"
                     /locus_tag="POB34_001208"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDI5918195.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGISPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            7609..9300
                     /gene="fruA"
                     /locus_tag="POB34_001209"
     CDS             7609..9300
                     /gene="fruA"
                     /locus_tag="POB34_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HDI5918196.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            9724..10665
                     /locus_tag="POB34_001210"
                     /pseudo
     CDS             9724..10665
                     /locus_tag="POB34_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
     gene            10653..11591
                     /gene="psuG"
                     /locus_tag="POB34_001211"
     CDS             10653..11591
                     /gene="psuG"
                     /locus_tag="POB34_001211"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HDI5918197.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMLFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAAIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            11685..12935
                     /locus_tag="POB34_001212"
     CDS             11685..12935
                     /locus_tag="POB34_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDI5918198.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(13132..13830)
                     /gene="yeiL"
                     /locus_tag="POB34_001213"
     CDS             complement(13132..13830)
                     /gene="yeiL"
                     /locus_tag="POB34_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HDI5918199.1"
                     /translation="MKEIHNNDLKLQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            13960..14901
                     /gene="rihB"
                     /locus_tag="POB34_001214"
     CDS             13960..14901
                     /gene="rihB"
                     /locus_tag="POB34_001214"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HDI5918200.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            15001..16251
                     /locus_tag="POB34_001215"
     CDS             15001..16251
                     /locus_tag="POB34_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDI5918201.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWTDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(16307..17395)
                     /locus_tag="POB34_001216"
     CDS             complement(16307..17395)
                     /locus_tag="POB34_001216"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDI5918202.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDET
                     VLAINDTHLLEQLSPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLAGEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(17398..18255)
                     /gene="nfo"
                     /locus_tag="POB34_001217"
     CDS             complement(17398..18255)
                     /gene="nfo"
                     /locus_tag="POB34_001217"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDI5918203.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(18329..19378)
                     /locus_tag="POB34_001218"
     CDS             complement(18329..19378)
                     /locus_tag="POB34_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HDI5918204.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            19477..20358
                     /locus_tag="POB34_001219"
     CDS             19477..20358
                     /locus_tag="POB34_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI5918205.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            20563..22032
                     /gene="lysP"
                     /locus_tag="POB34_001220"
     CDS             20563..22032
                     /gene="lysP"
                     /locus_tag="POB34_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HDI5918206.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            22326..24317
                     /gene="cirA"
                     /locus_tag="POB34_001221"
                     /pseudo
     CDS             22326..24317
                     /gene="cirA"
                     /locus_tag="POB34_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(24349..25185)
                     /gene="yeiG"
                     /locus_tag="POB34_001222"
     CDS             complement(24349..25185)
                     /gene="yeiG"
                     /locus_tag="POB34_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDI5918207.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            25443..26111
                     /gene="folE"
                     /locus_tag="POB34_001223"
     CDS             25443..26111
                     /gene="folE"
                     /locus_tag="POB34_001223"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDI5918208.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            26128..27285
                     /locus_tag="POB34_001224"
     CDS             26128..27285
                     /locus_tag="POB34_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HDI5918209.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLVGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            27427..28467
                     /gene="galS"
                     /locus_tag="POB34_001225"
     CDS             27427..28467
                     /gene="galS"
                     /locus_tag="POB34_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HDI5918210.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            28747..29745
                     /gene="mglB"
                     /locus_tag="POB34_001226"
     CDS             28747..29745
                     /gene="mglB"
                     /locus_tag="POB34_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDI5918211.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            29806..31326
                     /gene="mglA"
                     /locus_tag="POB34_001227"
     CDS             29806..31326
                     /gene="mglA"
                     /locus_tag="POB34_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HDI5918212.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            31342..32352
                     /gene="mglC"
                     /locus_tag="POB34_001228"
     CDS             31342..32352
                     /gene="mglC"
                     /locus_tag="POB34_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HDI5918213.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(32423..33658)
                     /gene="preA"
                     /locus_tag="POB34_001229"
     CDS             complement(32423..33658)
                     /gene="preA"
                     /locus_tag="POB34_001229"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HDI5918214.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASSMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(33652..34890)
                     /gene="preT"
                     /locus_tag="POB34_001230"
     CDS             complement(33652..34890)
                     /gene="preT"
                     /locus_tag="POB34_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HDI5918215.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSSCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMVIYQPGTKMLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSNVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(35209..35448)
                     /locus_tag="POB34_001231"
     CDS             complement(35209..35448)
                     /locus_tag="POB34_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HDI5918216.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(35451..36170)
                     /gene="sanA"
                     /locus_tag="POB34_001232"
     CDS             complement(35451..36170)
                     /gene="sanA"
                     /locus_tag="POB34_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HDI5918217.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(36320..37204)
                     /gene="cdd"
                     /locus_tag="POB34_001233"
     CDS             complement(36320..37204)
                     /gene="cdd"
                     /locus_tag="POB34_001233"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDI5918218.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVTLECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(37334..38029)
                     /locus_tag="POB34_001234"
     CDS             complement(37334..38029)
                     /locus_tag="POB34_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HDI5918219.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(38026..38424)
                     /locus_tag="POB34_001235"
     CDS             complement(38026..38424)
                     /locus_tag="POB34_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDI5918220.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            38663..39610
                     /gene="dusC"
                     /locus_tag="POB34_001236"
     CDS             38663..39610
                     /gene="dusC"
                     /locus_tag="POB34_001236"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HDI5918221.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(39981..40064)
                     /gene="yohP"
                     /locus_tag="POB34_001237"
     CDS             complement(39981..40064)
                     /gene="yohP"
                     /locus_tag="POB34_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HDI5918222.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            40288..>40986
                     /locus_tag="POB34_001238"
     CDS             40288..>40986
                     /locus_tag="POB34_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDI5918223.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEG"
BASE COUNT        10067 a        10095 c        10748 g        10076 t
ORIGIN      
        1 cagccacaaa aaggccttac tttcgaccag tttctgatta aagaaataaa aaatatccgc
       61 atcaattggc aaccagaatc catgattaac gggaatatac caggaaagaa acagcgcgag
      121 gccgacaata ttcaacaaca ctatttgcgg caaattttta atcatatttt ctctaattat
      181 cttactgaaa aacgcaggca accttacgcg gtagtcgcta aacgaagctt caacagcgcg
      241 ctctgcaaag cattccagtc agcttcgctg ctggaaatca gctcaatacg gctgtccggt
      301 ggcgcaacgt tttgcgtttc aatgtgcagg tcatcgccct gacggttgat tcgtaccagc
      361 ccttcgggaa tacgcagcac gcctttgacg cgttccaccg gtgcaagtcg cgcccattcc
      421 agaatgccaa tggtgtcgaa taccgtatca gcgtcgaata tccagccgca ggcctgatat
      481 ccttgcccgc tgttcagact gcgacgccag cgttgatgct ctggcaggct taacgctgct
      541 aacccttttt tcacgacatg ctgatgagaa tgcgcggcgc tggcgggcaa ctcggctaaa
      601 ttgcgacgcg gcaaatccag aagatgaccg tcaactttcc catgctcact gtgaattaat
      661 tgtcgatcgc caccattttg ctgccaccaa cgctgtagcg cttgctcact ttcgggcgtc
      721 gcacggtcgg atttattggc gacaatgatg tctgcggcag ccagctggtc acggaagttt
      781 tcattgctgg cgcttttttc gtccagcagc aggcgtggat cgagaatgca caaggtggcg
      841 cgcagatcta tccacggttc atagactggt gcggttaaca gatcgaggat ctgtttcgga
      901 tggcccagcc cggtcggctc tatcaacaag cggtctggtt ttccctgacg cagtaaggta
      961 ttcaacccta cctgcatggg taaaccatta acgcagcaca tgcagccgcc ggggatctct
     1021 ttcagcaatg cgccgctatc ggcgagcaaa gcaccatcaa ttccgacttc cccaaattca
     1081 ttaaccagta ccgcccattt ttcgttggga tctttatggg ctaacagatg aagaatcgac
     1141 gtggttttcc cgctgccgag aaaaccggtg atgaggttgg tcctggtcac aacggctccg
     1201 caatactctg gtaagttaac tgagcgttaa tcctggctaa aaaaggcaga aaagagaagg
     1261 ggagagcgga cgaaggtgag cttcatccgc tttaaccgca gaaatcgtta aattttgagg
     1321 gtgtttaata gcgcctgatg tgcgccgaat tgtacgattt gttgccaggc ttgttcgatg
     1381 gcctgcacaa aatgcgggtt atctggcaga tcatcaccga aaacttcacg cagggaaagc
     1441 agggcggtta cgcgttgttc actgctgctg cccgcaacaa gttcgcgaat tttatcgcta
     1501 agcggatcgc gaacatcaat ggcatttccg gcatcatcaa cgccgctgac gtaacgcatc
     1561 cagccagcaa cgcctaatgc cagcaacgac cagtccgttt cgcgccccag atgtatgcga
     1621 atacctgcca gcatacgttg cggtaatttc tggctaccat ccattgcgat ttgccaggtc
     1681 ttatgtttca gcgccggatt agcaaaacgt gcaattaact tgtccgcata ttgtgttaaa
     1741 tcgacatctt taatttgtag tgtcggcgct tgctcattca gcattaatgt tctggcggca
     1801 tggcgaaatg cgcgatcctg catgcaatca ctgatatggg cgaatcctga aaggtaaccc
     1861 agataagcga gaaaagagtg gctgccatta agcatacgca gtttcatctc ttcccatggc
     1921 aggacatcat tcaccatttg tacacctgcg acttcccagg caggacgccc agcgacgaag
     1981 ttatcttcca ccacccactg gataaacggt tcgcagctaa tcgcgcaggg atcatttacc
     2041 cccagatgct ggctgatttc tgccagtgat tcgtcggttg cagccggaac aatgcggtcg
     2101 accatggttc ccggaaaact gacgtgttct tttatccacc cggcgagttc tggcgaacgt
     2161 ttttctgcca ttcccagcac cgcgtttttc accacatggc cattgtcggg aatattgtcg
     2221 caggagagca cggtaaacgg tgtaaggccg cgctcacggc ggcgtttcag tgcttcgacg
     2281 agaatacccg gtgcggagtg aggttcttca ggggtttgta gatcgtgaat aatccgcgga
     2341 ttactggtgt cgagtgcacc ggtggccggg tcaatacaat agcctttttc ggtaatcgtc
     2401 agggaaacaa ttgccacctg tggctcgcaa aatttctcaa taattgccgc taaggaatcc
     2461 agttttgcat taaggcattc gtgaacggca ccgacaatta tcacctgatt gccgtccgca
     2521 cctttctcca gcacggtata taaatggttc tgtgcgcgga gctggctcat cagttgatca
     2581 ccgctgaaca agctgatttc acagatcccc cagtcgccgc cctgggcatt cagcacacga
     2641 tcggtcagta acgcctgatg agcgcggtga aaggcgccaa agccaaaatg aacgatacgt
     2701 gattgcaact gctggcgatc gtagcgtgga gcgtgtacat gatgcgggag cgtaacggag
     2761 gcaattgtct tcataggagt cacgtcttag taacgataaa ttaacaatga cagtgtaaag
     2821 ttatatgaca gcaattgaaa ttggtctgga ttgttaatgc gatgaagtgt gagggcgatc
     2881 aatcaacgag tgaaaattga gggcgtaagg tgttcaggaa gcgcaaaata gaagattggt
     2941 ataataaatt aaaaaaggga cagcctgagc tatccctttt ctgcatgcgg ctgaagttag
     3001 tcagcacgcc ccatataacg gcgttcttcg atatggatac gaattttttc gcccgggctt
     3061 aagtattctg gtacctgaat caccagacca gtgctcaatg tcgccggttt gttacgggcg
     3121 ctggcagatg cccctttgat gcctggtgcg gtttcaacga tttccagatc aacggtctgc
     3181 ggcagctcaa gcgccagcag ttgaccgtcc caggtcagca cctgcatgtc cggcatgccg
     3241 ccctctggca taaacagcaa ctcttcttca atctgatctt tggtgaaggt atacggggta
     3301 tagtcttctt tatccataaa gacatattcg ttgccatcga cataggagaa gtcaacgtaa
     3361 cggcgggtca gcgtcacggt gtcaacgata tcatcacctt tgaaacgctc ttctactttc
     3421 agcccggtac ggacatcaga aaaacgcatt ttgtacagcg tagcggcacc gcgggcagtg
     3481 ggcgactgaa tatcaatatc cttaaccagc agcagtttgc cgttgtaatt cagtaccata
     3541 ccttttttaa tttcgttcgc tcttggcatc gaaaaattcc tgtagtcgaa aatgtcaaaa
     3601 atatcgcgac aaagtactcg cgacgggcct ttcgggcaag tggtattcgc acttttgctg
     3661 gtgcaaaaaa ggtggtactg tgcgcgctca tcaatccggt ggttaacctt aagagaacaa
     3721 cgtatggaat gccgtccggg ttgcggggcg tgttgcactg ccccttcaat ttccagccct
     3781 attcccggta tgccagatgg caagcccgcc aatacgccct gcattcagct tgatgaacag
     3841 caacgctgta aaattttcac ctcgccactg cgaccgaaag tctgtgcagg cttacaagcc
     3901 agcgctgaaa tgtgcggtaa ctcgcgtcag caggccatga cctggcttat cgatctggaa
     3961 atgctgaccg cgccttaaac atctttaatc cgcagtaagc aaaacagagt ggcgataatc
     4021 atcaccatcg caaaccagaa cacagcgtga taattccaga tctcggcgac gatgcccgcc
     4081 actgatcctg cgatgatcca gcccacgcgc gacgtgttgg tatagagcgt ggtggctgaa
     4141 cccgcctgac cgggcatcag atcctgaaaa tagagcatcc cgatgccgcc cagaatgcca
     4201 ataaaaatag catttagcag ctgcaagccc aacagaatga caggtgaatg cgccatcagc
     4261 attcctgcgt aaaaacagac gccacccacg gcagcaacgc gcattaagaa acgcttaccc
     4321 agacgtttgg cgaaatatcc ggcaatcaac atcgtcggga tttccagccc ggcggcggtc
     4381 cccatcatca caccggccag tttctcggga agatgcagtt cgttgataat aaatagcggc
     4441 atgttgatga tgtacaggct gttcgagccc cacatcaatg tacaaatgac aaacagcagc
     4501 agcgtatcgc gacggttacg gcgcggcgct tcaaccgtgc cggtcgccag cggaagctct
     4561 ttttgcatcg acggtaaaaa cagccacacc atcacgccgc aaacaataaa tgctaccgct
     4621 gcgctcagat acattaccgt aaagctgaaa cccatcgcta aggcataagc cagcggtggg
     4681 ccaatgaccc atgccagtga aacctgagcg cgtaaaaaag agctgaacat caccgcttca
     4741 cgtccggttt tgtcggcatg ttcacgggca agggcaaaca tttgcgggtt agcggtcgag
     4801 ccaaagctgc taagaaagac gccaacgaat agcaaaacaa agtagttgcg attccaggca
     4861 aaaagggtgc aggccagcac gcctaacagg cagcaaaaga caatcagcga tttgcgatcg
     4921 ccgcgcttat cagagcgccc ggcgagaaac tgacttacca gaatcccaat gacagcgctg
     4981 ccggtaaaga agaatcccac catcgccgga cgggcatgta cttcatcagt aagaaaaata
     5041 ctgagtgtcg gggtttgcag agcgcccgca ataccggtga gaaaggcaac gattaaaaac
     5101 gccgtcgagg tcaggtcaaa cgatttcgcg ctggagactg cgggggagtt atgcatgctg
     5161 gtcgatcact ggaagaggaa gacgcggagt ttacgctgct tgtcagaaaa taaacagaca
     5221 gcgaaatcaa aagcgccacg aataatcaaa atgccatttc actgttgctg attggctgct
     5281 gacgctgctg aaatgaaatt gctgataacg gattttccca tcagcaatta ggaaaaatga
     5341 caaaaaattg tgcagcacat caaacttttg ctcataactt tacggctttc cttgcgtgct
     5401 gaatcagaag tattatgctt tcttgaaacg tttcagcgcg atcttgtctt taaccctaag
     5461 ccaggttggc gctttttttc tcatagaggc tgaatcgttt caattcagca agagaggaga
     5521 actatgttcc agttatccgt acaggacatc catccgggcg aaaaggccgg agacaaagaa
     5581 gaggcgattc gccaggtcgc tgcggcgctg gtgcaggccg gtaacgtagc agaaggctac
     5641 gtcaatggca tgctggcgcg cgaacagcaa acctcaacgt tcctcggcaa tggtattgct
     5701 attccacacg gcaccaccga cacccgcgat caggtgctga aaaccggcgt tcaggtattt
     5761 cagttcccgg aaggcgtcac ctggggtgac ggtcaggtag cgtacgtggc gatcggtatt
     5821 gctgccagct cggatgaaca tctgggcctg ctacgccagc tgacccacgt actgagtgat
     5881 gattccgttg ctgaacaact gaagtcagca acaacagcag aagaacttcg cgcattgctg
     5941 atgggcgaaa agcagagtga gcagctgaag ctcgacaacg aaatgctgac actggatatc
     6001 gtcgccagcg atctgctgac tcttcaggcg ctgaacgctg cgcgtctgaa agaggcgggg
     6061 gcagttgacg ccactttcgt caccaaagcc atcaatgaac aaccgctgaa cctcggacag
     6121 ggcatctggc tgagcgatag cgccgaaggc aatctgcgta gcgcgattgc ggtaagccgt
     6181 gcggcaaatg cttttgatgt ggacggcgaa acggcagcca tgctggtgag tgtagcgatg
     6241 aatgacgatc agcccatcgc ggttcttaag cgtctcgctg atttgttgct cgacaataaa
     6301 gctgaccgct tgctgaaagc ggatgcggca acgttgctgg cactgctgac cagcgatgat
     6361 gcgccgaccg acgacgtgtt aagcgcggag tttgtggtgc gcaatgaaca cggcctgcat
     6421 gctcgtccag gtaccatgct ggtcaatacc attaaacaat ttaacagtga tattaccgtg
     6481 acaaaccttg atggcaccgg caaaccggca aacggacgta gtctgatgaa agttgtggca
     6541 cttggcgtta agaaaggtca tcgcctacgc tttaccgccc agggtggaga tgctgaacag
     6601 gcgctgaaag ccattggcga cgctatcgct gctggtcttg gggagggcgc ataatgagca
     6661 gacgtgttgc tactatcacc cttaatccgg cttatgacct tgttggtttc tgcccggaaa
     6721 ttgaacgcgg cgaagtgaac ctggtgaaaa ccaccggtct gcatgcggcg ggtaaaggca
     6781 tcaacgtggc caaagtatta aaagacctgg gaattgatgt caccgttggc ggcttcctcg
     6841 gtaaagacaa tcaggatggt tttcagcaac tgttcagcga gctgggcatt gccaaccgtt
     6901 tccaggttgt acaggggcgc acccgaatta acgttaagct gacggaaaaa gacggtgaag
     6961 tgaccgactt caacttctcg ggttttgaag tcacccccgc cgactgggaa cgctttgtga
     7021 ctgattctct gagctggctc ggtcagttcg atatggtctg tgtcagcgga agcttaccgt
     7081 caggcatcag cccggaagcg ttcaccgact ggatgactcg cctgcgtagt cagtgtcctt
     7141 gcattatctt tgatagtagc cgtgaagcgt tagtagcagg tttgaaagcg gcaccgtggc
     7201 tggtgaaacc taaccgccgc gagctggaaa tctgggcagg ccgtaaactg cctgaaatga
     7261 aagatgtgat tgaagctgca catgcgctac gtgaacaagg catcgcgcat gttgttattt
     7321 cactgggtgc cgaaggcgcg ctttgggtta atgcctccgg cgaatggatc gccaaaccac
     7381 cgtcagtcga tgtcgtaagc accgttggcg caggggattc tatggttggt ggcctgattt
     7441 atggcttgct gatgcgtgaa tccagtgaac acacactgcg tctggcgaca gctgttgcag
     7501 ccctggcggt aagtcaaagc aatgtgggta ttaccgatcg tccgcagttg gccgcaatga
     7561 tggcgcgcgt cgacttacaa ccttttaact gacagcagga gaggcataat gaaaacgctg
     7621 ctgattattg acgctaatct cggtcaggca cgcgcctata tggcgaagac cctgctgggc
     7681 gcggcggcgc gaaaagcaaa actggaaatc atcgacaatc cgaacgacgc tgaaatggcg
     7741 attgttctcg gtgattccat cccgaacgac agcgcgctga acggtaaaaa tgtctggctg
     7801 ggtgatattt cccgggcagt tgcgcaccct gagctgttcc tgagtgaagc caaaggccat
     7861 gcgaaacctt acactgcgcc ggtcactgcg acagcaccgg ttgccgccag cggtccgaaa
     7921 cgcgtagttg cggtgactgc ttgcccgact ggcgtagcac acacctttat ggcggctgaa
     7981 gccattgaaa ccgaagcgaa aaaacgtggc tggtgggtga aagttgaaac ccgtggttct
     8041 gttggcgcgg gtaatgcaat cactcccgag gaagtagccg cagcggatct ggtgattgtg
     8101 gcggcagata tcgaagtgga tctggcgaaa tttgctggta aaccgatgta tcgtacctct
     8161 accggtctgg cgctgaagaa aaccgcgcag gaactggata aagcggttgc tgaagcaacg
     8221 ccgtatgaac cagcgggcaa agctcaaaca gcgaccactg aaggtaagaa agagagtgca
     8281 ggcgcttacc gtcacttgct gacgggcgtc tcttacatgc tgccgatggt tgttgcaggt
     8341 ggtctgtgta tcgcgctttc ttttgctttt ggtatcgaag cgtttaaaga gccgggtacg
     8401 ttggcagcgg cgctgatgca gattggcggt ggttcagcct ttgcgctgat ggtgccggta
     8461 ctggcaggtt atattgcctt ctccattgcc gatcgtccgg gcctcacgcc ggggctgatt
     8521 ggcggtatgc tggctgtcag caccggttct ggcttcattg gcggtattat tgcgggcttc
     8581 ctggctggtt acattgcgaa gttaatcagt acgcaactga aactgccaca gagtatggag
     8641 gcgctgaaac caatcctgat cattccgcta atttccagtc tggtggtcgg tctggcgatg
     8701 atctacctga tcggtaaacc ggttgctggc attctcgaag ggctgactca ctggctgcag
     8761 accatgggga ctgcgaatgc ggttctgctg ggggcgatcc tcggtggcat gatgtgtact
     8821 gacatgggcg gtccggtaaa caaagcagcg tacgcattcg gtgtgggtct gctgagtact
     8881 caaacctatg gcccgatggc ggcgattatg gcggcaggta tggtgccacc gctggcaatg
     8941 ggtctggcaa caatggtggc gcgtcgcaaa ttcgacaaag cgcagcagga aggtggcaaa
     9001 gccgctctgg tattgggact gtgcttcatt tcggaaggtg caattccgtt tgctgctcgt
     9061 gatccgatgc gtgtgctacc gtgctgtatc gtgggtggtg cgctgactgg cgcaatctca
     9121 atggcgattg gtgcgaaact gatggcacca cacggtggtc tgtttgttct gctgatccct
     9181 ggcgctatta cgccggtact gggttacctg gtagcaatta ttgccggtac gctggtggcg
     9241 ggtttggcct atgccttcct gaaacgtccg gaagtggacg cagtagcgaa agcagcgtaa
     9301 taaaaggtgt gttacagggc agaaatttcc tgccctgtat cattggatga gcgcaaaggg
     9361 aatgaccagc ttgtgaatgt aattttatga atttaaatgg ttttatcttt cagtagaaac
     9421 ttgaaacgtt catcaaacac taaggttatt tcctcaaggg cagattattt tctgcccttt
     9481 tttattgttc ctgttctgtg catcccgtca caaattcctc gcttttgtaa ttcaggcatt
     9541 taatcgcatt ttgtgagcct gctcaaatct gcccatttag cctctctggc ggtaatttac
     9601 tttacgtaaa acatgcatac tatgagcaca tgtttaaatg tgaacacatg tttaaaccgt
     9661 ctttgcagta tctatattct tgtggattga cagggttggt aattttttac cagaggacgt
     9721 gctatgcgcg aaaaggatta tgtcgtaatt ataggctcgg cgaatattga tgtcgccgga
     9781 tattcacatg aatcattaaa ttatgcggat tcaaatccag gtaaaataaa atttacgcct
     9841 ggtggagtcg ggcgcaatat tgcacaaaac ctggcgttgc tggggaacaa agcctggcta
     9901 ctgagcgccg taggcagtga cttttatggt caatcgctgc taacgtaaac caatcaatct
     9961 ggcgtttatg tcgataaatg cctgattgtg ccaggagaaa atacgtcaag ctatttatca
    10021 ttactcgata ataccggtga aatgctggtt gctataaatg acatgaatat tagcaacgct
    10081 attacagctg aatatctcgc acagcaccgt gaatttattc agagggcaaa ggtcattgtc
    10141 gcagactgta atatcagtga agaggcactg gcatggattc tggataatgc cgccaacgta
    10201 cccgtatttg tcgatccggt ttcagcgtgg aaatgtgtca aagtgcgcga ccgtctaaat
    10261 cagatccaca ctctcaagcc aaaccgcctt gaagcggaaa ccctgagtgg gattgcgctg
    10321 tcagggcgtg acgatgtggc aaaagttgct gcctggttcc atcaacatgg cctgaaccga
    10381 ctggtattga gcatgggcgg cgacggcgtt tattacagcg atatcagcgg tgaaaatggc
    10441 tggtctgcgc cgatcaaaac caatgttatt aatgttaccg gagcgggcga tgccatgatg
    10501 gcgggacttg cttcgtgttg ggtagacgga atgccgtttg ccgaatctgt tcgtttcgca
    10561 cagggatgtt cgtcaatggc gctctcctgt gaatacacca ataaccccga tttatcgatt
    10621 gccaacgtta tatcgttagt ggagaacgca gaatgtctga attaaaaatt tcccctgaat
    10681 tattacaaat ttccccggaa gtgcaggacg ctttaaaaaa caaaaaaccg gttgtggcgc
    10741 tggaatcgac cattatttct cacgggatgc tgttcccaca aaatgcccag accgcaattg
    10801 aagtagaaga aactattcgt aaacagggcg cagtacctgc cactatcgcc attattggcg
    10861 gcgtgatgaa agtgggttta agcaaagaag aaattgaatt actgggtcgt gaagggcata
    10921 acgtgaccaa agttagtcgt cgcgatttac cttttgttgt tgccgccgga aaaaatggcg
    10981 caaccactgt ggcttcaacg atgattattg cggcgcttgc cggaattaaa gtatttgcca
    11041 ccgggggaat tggtggtgtg catcgcgggg cggaacatac cttcgatatt tctgccgatt
    11101 tgcaagaact ggcaaatact aatgtcaccg ttgtttgtgc cggggcgaaa tctattctcg
    11161 atttaggatt aacaacagaa tatttagaaa ccttcggtgt gccgttaatt ggctatcaga
    11221 caaaagcgct gcctgcgttt ttctgccgta ccagcccgtt tgacgtcagc attcgtctcg
    11281 acagcgccag tgaaattgcc cgtgcaatgg cggtgaagtg gcaaagcggg ctgaacggtg
    11341 gcctcgtggt agcgaacccg atcccggaac agtttgcgat gccggaacac actatcaatg
    11401 cggcgatcga tcaggcggta gctgaagctg aagcgcaggg cgttattggt aaagaaagta
    11461 caccgttcct gctggcgcgc gttgctgagc tgaccggcgg tgacagcctg aaatccaata
    11521 tccagctggt gttcaacaac gccattctgg cgagtgaaat tgccaaagaa taccagcgtc
    11581 tcgcgggtta atttctgacg ggcagaaagc cgtcgcccgt actcatcaag cataccaaaa
    11641 cctggcgctg acgccgggtt tactggcatg aagggaaaat cattatggat ataatgagaa
    11701 gtgttgtggg gatggtggtg ttactggcaa tagcatttct gttgtcagtg aataaaaaga
    11761 gcatcagttt gcgcacggtt ggagccgcac tgctgctgca aatcgctatt ggtggcatca
    11821 tgctctactt cccaccggga aagtgggcag tagaacaggc ggcattaggc gttcataaag
    11881 tgatgtctta cagtgatgcc ggtagcgcct tcatttttgg ttcgctggtt gggccgaaaa
    11941 tggatgtcct gtttgacggt gcgggtttta tcttcgcctt tcgcgtactt ccggcgatta
    12001 ttttcgttac tgcgctcatc agtctgctgt actacattgg cgtgatgggg ctgctgattc
    12061 gcatccttgg cagcattttc cagaaagctc ttaacatcag caaaatcgaa tcttttgtcg
    12121 cagtcacaac tattttcctc gggcaaaatg agatcccggc gatcgttaaa ccgtttatcg
    12181 atcgcatgaa tcgcaacgag ttgtttaccg caatttgtag cgggatggcg tccattgctg
    12241 gttcgatgat gattggttat gccggaatgg gcgtaccaat tgactacctg ttagcggctt
    12301 cgctgatggc tattcctggc ggtatcctgt ttgcacgtat tcttagtccg gcgacggaac
    12361 cttcgcaagt tacgtttgaa aatctgtcgt ttagcgaaac accgccaaaa agcattattg
    12421 aagctgcggc gaacggtgca atgaccgggc tgaaaatcgc cgccggtgtg gcgacggtgg
    12481 taatggcatt tgtggcgatc attgcgctga tcaacggcat tattggtgga gttggcggct
    12541 ggtttggttt tgccaatgtc tctctggaaa gcattttcgg ctatgtgctg gccccgttgg
    12601 catggattat gggggtggac tggagtgatg caaatcttgc cggaagtttg attgggcaga
    12661 aactggcgat taatgagttt gttgcttatc tcaatttctc gccctatctg caaacgagcg
    12721 gcacgctgga tgtcaagacc attgccatta tctcgttcgc cctctgtggg tttgcaaact
    12781 ttggttctat cggtgttgtc gttggagcgt tttcggctat ttcgccaaaa cgcgcgccgg
    12841 aaatcgccca gcttggttta cgggcgctgg cggcggcgac gctttccaac ctgatgagcg
    12901 cgaccattgc cgggttcttt attggtttag cttgagtttc attgccggat gtactcgctt
    12961 attaagcgtt gcatccggca atcgcgtact atactcgtca tacttcaagt tgcatgtgct
    13021 gcggctgcgt tcgctcaccc cagtcactta cttatgtaag ctcctgggga ttcactcgct
    13081 tgccgccttc ctgcaactcg aattatttag agtatattcc agattatttt tttactgcat
    13141 catcccggag aatttattct ccgggtccat ctccagcgcc agtcctgaca actgctttct
    13201 gtttttaatt agatatcctt tcttgctttt aattaataaa ccgtcgtgaa tgaattgcgc
    13261 gagaacatat aaaagatgtc gataagaaac gcctaaatac tctgccgctt gcgtatgctt
    13321 ttcgtgataa agatcacctt cttgcgagag taatataaat gctgccaggc gattcactaa
    13381 cggaaatgat tgattctgag ttaaagaaac aatattacga taatttttat gacttaaggt
    13441 gacgcaaagt tttcgtaaaa atagcgtgtc gtttaataac agcggacggt aatgtttcat
    13501 agggagcgca aggcaccaac actcttcaat cgcctgtact gcacgtggtt catggtcttt
    13561 atcgattaac tcaatctcgc caataaaaca gggggcggca aagaaatcga tcagcgacac
    13621 gcgaccatta gctagcgtgg cgtaaagcct ggcgcggcct cgcgtcaggt aaaacagcca
    13681 ggacggctgt tgcccttcct gcacaatgta gtcacgcgct aaaaaatgaa acagccgcgt
    13741 atcggctgaa acatccgtta aaaagcaatc cttaaacgcg gattcactca tcaattgcag
    13801 cttaagatca ttattgtgga tttctttcac gatgtttatc cgctatgaga tttctcatat
    13861 ttagcatacg tacgctgttg ttaagattca atcatcggcc cggtcgaatt ttctttgctg
    13921 tagaaaaaat aacgcaactg gaaacagagg aaataaacaa tggaaaagag aaaaattatt
    13981 ctggattgtg atccgggtca tgatgatgct attgcaatga tgatggcagc gaaacatccg
    14041 gcaatagatt tattaggcat cactattgta gcgggtaatc agacgcttga taaaacatta
    14101 attaatggcc tgaatgtttg ccagaaactg gagattaatg ttccggttta tgcggggatg
    14161 ccgcagccca ttatgcgtca acaaatcgtt gccgataata ttcacggtga aaccggactg
    14221 gatggcccgg tattcgagcc gctgacccgc caggcagaaa gtacccacgc ggtgaaatac
    14281 atcattgata ccctgatggc aagcgatggc gatatcactc tggtgccggt tggtccgctt
    14341 tcaaatatcg cggtggcaat gcgtatgcaa cccgcgatcc tgcccaaaat ccgtgaaatt
    14401 gtgctaatgg gcggcgctta cggtacaggc aacttcacgc catctgccga gttcaacatc
    14461 tttgctgacc cggaagccgc acgcgtggtg ttcacctctg gcgttccatt agtgatgatg
    14521 ggcctcgatc ttaccaacca gaccgtttgc accccggacg taattgctcg gatggaaagg
    14581 gcaggcggcc ctgccggaga gctgttcagc gacatcatga acttcactct caaaacgcag
    14641 ttcgaaaact acggcctggc tggcggcccg gtgcacgacg ccacctgcat cggttatctg
    14701 attaaccctg atggcattaa aacccaggag atgtacgtcg aagtggacgt caacagtggc
    14761 ccttgttatg ggcgtaccgt ctgcgacgag ctgggcgttc ttggcaagcc cgctaatacc
    14821 aaagtcggca tcactattga taccgactgg ttctggggct tagtcgaaga gtgcgtgcgc
    14881 ggctacatca aaacccatta agtgttgata acaagccggg ccggtatcgc aagatgcagg
    14941 cctggctatt tctgaggggc agaaagccgt cgcccaatat gacatgaaga gataatcact
    15001 atggatgtca tgagaagtgt tctgggaatg gtggtattgc tggcaattgc atttttgctg
    15061 tcagtaaaca agaagaagat cagcctgcgt accgttggcg cggcgttagt gttacaggtt
    15121 gtgattggcg gcattatgct ttggttaccg ccagggcgtt gggtcgctga aaaagtcgct
    15181 tttggcgtgc ataaagtgat ggcgtacagc gacgcgggta gcgcatttat cttcggttcg
    15241 ctggtcgggc cgaaaatgga cacgctgttt gatggcgcag gatttatctt tggtttcaga
    15301 gtattaccgg caattatctt cgtcaccgcg ctggtgagta ttctctatta catcggtgtg
    15361 atggggatct taattcgcat tctcggcggt atcttccaga aagcattaaa tatcagcaag
    15421 atcgagtcat tcgtcgcggt cactaccatt ttcctcgggc aaaacgaaat tccggcaatc
    15481 gtcaaaccct ttatcgatcg gctgaatcgc aatgaattat ttacagcgat ttgtagtggc
    15541 atggcctcaa ttgccggttc gacaatgatt ggttacgccg cactgggcgt acctgtggaa
    15601 tatttgctgg cggcatcgtt aatggcgatc ccaggcggga tcttgtttgc ccgcctgtta
    15661 agcccggcta cggaatcttc gcaggtttct tttaataacc tctctttcac cgaaacaccg
    15721 ccaaaaagca ttattgaagc cgccgcgaca ggggcgatga ccgggctgaa aatcgctgcg
    15781 ggtgtggcaa cagtggtgat ggcatttgtt gccatcattg cgttaattaa cggtattatc
    15841 ggcggcgttg gcggctggtt tggttttgaa catgcctcgc tggagtccat tttaggttac
    15901 ctgctggccc cactggcgtg ggtaatgggt gttgactgga ctgatgcgaa tcttgccggg
    15961 agtttgattg gacagaaact ggcaataaat gaatttgtcg cttatctcaa tttctcaccc
    16021 tatctgcaaa cggctggcac tctggatgct aaaaccgtgg cgattatttc cttcgcgttg
    16081 tgcggtttcg ctaactttgg ttctatcggg gtggtggtgg gggcgttttc tgcggttgcg
    16141 ccacaccgtg cgccggaaat cgcccagctt ggtttacgcg cgctggcggc ggcgacactt
    16201 tctaacctga tgagtgccac cattgccggg ttctttattg gtttagcgta gtgtgcgatt
    16261 tatgagccgg ataagatgcg gtcaagcgtc gaatccggca acattgttac accattggca
    16321 ctaatgaaag cgcgttatcg gcagacaggg tagggttgtt taagctgccg ctggcgcgtg
    16381 aaattgccgc gcaggccatc gcaaaacggg cgctgtcacg gaaattgttt ccttccagaa
    16441 agctatatac caggcccgcc ataaaaccat cgtcagcacc aaaactgtct actgtcgtat
    16501 gcgctggtgc ggtcagcaaa aattgttctc catccttttc gctgcaataa actgactcat
    16561 cgggcaaata aacaaacagt tgctgaacac cttgctgatg caacgcattc actgcagcat
    16621 tacggtcagc atcgctggtg atcgcctgtc cccataaaat ttccagctcc ggtaaagtgg
    16681 gtttcagggt gtgaatatgc gccagccagt gtttgatttt gcccgctttg aattctgaaa
    16741 cggtatcgac aaacaccggg atctcaccag cgagggtaaa gacccattcc agcgcctcgg
    16801 ctgtcaggtt gcaatcagcc agtaccacgc ccgcatgacg aagtaaatcg cgcgacccgt
    16861 tcaataattg cggcgataac tgttccagca gatgggtatc gttaatcgcc agcacggttt
    16921 catcgtctcg attggcaatt gccagatacg tcgatgtgct ttgaccatgt aaacgaacgc
    16981 agccggagac attcacgccg gcgcggcgcg tttcttccag gagcatttcg ccataaaagt
    17041 catcgccaat cactgaaagc aaatggacgt cgcggcctaa cagcgccaga ttgtgggcga
    17101 tattgcgtcc aacgccgcct gccgagcaat gaattgtacc gggatgagaa gccgcttgcg
    17161 ggtaatggat atccgccatc ccgcgaatat ccatattgat tgtccccacc actacgcagt
    17221 attcctgctc ggtgagaatg taacctttgc ctttaatccg ccctttgcgc attaaatcca
    17281 taatatgcgc cgcaacgcgc gaacgactga tttgcagcat gtccgcaatt tcgttctgct
    17341 gaatcagcgg gttacgccgt aaaattgcaa ggatctcctt ttcccggtta ttcatcttca
    17401 ggctaccgct ttttcagttt gttgcgcttt cagccaggcg atctcttctg cccagatatc
    17461 cgggttgatg gtttcaagga tcagcggaat gccgtcgaaa cggtcgtcct gcatgatcca
    17521 gcggaacgca tcatgaccga tattgccttc accgaggcta tgatggcggt caacgcggct
    17581 gccaaaggtg cttttcgcat cgttaaggtg catcccgcgc agatacttaa agccgacaat
    17641 acgggcaaaa tcagcgaacg ttttctcgca ttcggctgga gtacgcaaat catacccggc
    17701 agcgaaagca tggcaggtat caatgcagac gccgacacgg gatttatctt ccacgccgtc
    17761 gataatcgcc gcgagatgtt cgaatttaaa ccctaagtta ctgccctgac cggcggtgtt
    17821 ttcaatcacc gcggtcacac cttgagtttt atccagcgca atgttgatgg attcggcaat
    17881 acgcgcaagg caatcctctt ctgaaatctg catcagatgg ctgccagggt ggaagttgag
    17941 caaagaaagc cctagttgtt cgcaacgctg catttcatct ataaaggcat cgcgcgattt
    18001 ttccagagct tcagtgaccg gatgtccgag gttaatcaga taactgtcgt ggggaagaat
    18061 ttgcgccgat gtgtagtgat atttttcaca ggcggctttg aattcatcga tggtttgcgt
    18121 cgtgagcggt gcggcacgcc actgacgttg gtttttggtg aacaaggcaa acgcggttgc
    18181 gtcgatttcg gcggcgcgaa ttgcggcatt tgccagaccg ccagcagcac taacgtgcgc
    18241 tccaatgtat ttcatgcgag gactcctgtt aaacccgctg gaggaaaacg gtaatgatag
    18301 cgggttaaca ggagcaagat gtagtggttt atgcgatgac gctttgaatc acatagttaa
    18361 tcgcaccacc accaacaatc agccaggcaa acagtaccag tgccatcagc agaggtttcg
    18421 ccccggcttt tttcagcgcg ctgacgtgag tggtcagacc cagcgccgcc atcgccattg
    18481 ccagcaggaa ggtatccagc gttaccagca tgttcaccac gctctgcggt aacaggtgga
    18541 acgagttaaa gatggcaact acgatgaaca agatggcaaa ccacggaata gtgattttgc
    18601 ttttctcgcc gctgttcgcc ccagacagct gtttaacacg cgccgccagc aggatgagga
    18661 acggagccag catcatcacg cgcagcattt tggaaataac tgctgcgttt tccgcatccg
    18721 ggctgatggc atgacctgcc gccaccacct gcgccacttc gtgcacagta gaaccaatgt
    18781 agataccgaa agtttccgga ctaaaccatt gagacatcag cggatatatc gccgggtaga
    18841 ggaaaatcgc gacggtcccg aagataacaa cggttgcaac agccacggtt actttactgg
    18901 cttccgcttt cactaccggc tcagtcgcca gtaccgcggc agcaccacag atactgctac
    18961 cggcaccgat caaccagctg gtgtgcttat ccagaccaaa cactttctgc cccaggaagc
    19021 aagccagcag gaaggtactg gacagcgtca gcacgtcaat gatgatccca ctgataccga
    19081 catcggcaat ttgcgagaac gtcagacgga agccataaag aatgataccc agacgtaata
    19141 aatattgctt ggcaaacagc acaccaccgt cacagctttt ccagatgtgc gggtaaatgg
    19201 tgttgcctaa aaccatcccc aacaagattg cgagggtgag ggcactaaac ccggcacccg
    19261 caaccgcggg aatggaacca ccccacaggg cgaccccggt gataactgca ctcagggcta
    19321 accccggaat aaaatgccac agtgtacgat gttgtttctg taaggtgata ttcgtcataa
    19381 ccctctcctt taaccgggca taaggttacg actaactggt ttaaaaataa aattgattat
    19441 atatttataa ttaatcttta taagtggtaa gcgactatgc acatcaccct ccggcagttg
    19501 gaagtttttg cagaagtatt gaaaagtgga tcaaccaccc aggcgtcggt gatgctggcg
    19561 ttgtcgcaat cagcagtgag cgcagccttg accgacctgg aagggcagct tggcgtgcaa
    19621 ctgtttgatc gcgtggggaa aagactggtt gttaatgaac acgggcggct gctctatccg
    19681 cgagcgttgg cactgcttga acaggcggtt gaaatcgaac aactgtttcg cgaagacaac
    19741 ggcgcgattc gtatctatgc cagtagtacc atcggtaact acattctgcc tgcagttatc
    19801 gcccgttatc gccatgatta tccgcagttg ccgattgaac ttagcgttgg gaatagccag
    19861 gatgtgatgc aagcggtgct ggatttccgc gttgatattg gctttattga aggaccgtgc
    19921 cacagcactg aaatcatttc tgaaccgtgg ctggaagacg agctggtggt tttcgccgcg
    19981 ccgacttcgc cgttggcccg tggtccggtc accttagagc agctggccgc tgcgccgtgg
    20041 atcctgcgtg aacgcggttc cggcacgcgg gagattgtcg attatctatt gctgtcacat
    20101 ttaccgaagt ttgagatggc gatggaatta ggtaactccg aggcaatcaa acatgcggtg
    20161 cgtcatgggt tgggaattag ttgcctgtcg cgacgtgtga ttgaagatca attgcaggca
    20221 ggcacattaa gtgaagttgc ggtccctctc ccgcgcctga tgcgtacgtt gtggcgtata
    20281 catcatcggc aaaaacacct ttccaacgcg ctacggcgct ttctggacta ttgcgatccc
    20341 gcgaatgtac cgcgttaagg tgctgtacaa gaacatgctg gtgctgtgtc gatttcgtga
    20401 cgcagcgccc tcagcatgca ttcgccagaa aagagattgg ctgctttact tataatccct
    20461 gggcgatcat gaaggtgtct tataaccgtg tatttctgcc ggaaggattg ccaatcgtct
    20521 gctacaatcg cgcctcattt ttaagatgga tagcattttt gtatggtttc cgaaactaaa
    20581 accacagaag cgccgggctt acgccgtgaa ttaaaggcgc gtcacctgac gatgattgcc
    20641 attggcggtt ccatcggtac aggtcttttt gttgcctctg gcgcaacgat ttctcaggca
    20701 ggtccgggcg gggcattgct ctcgtatatg ctgattggcc tgatggttta cttcctgatg
    20761 accagtctcg gtgaactggc tgcatatatg ccggtttccg gttcgtttgc cacttacggt
    20821 cagaactatg ttgaagaagg ctttggcttc gcgctgggct ggaactactg gtacaactgg
    20881 gcggtgacta tcgccgttga cctggttgca gctcagctgg tcatgagctg gtggttcccg
    20941 gatacaccgg gctggatctg gagtgcgttg ttcctcggcg ttatcttcct gctgaactac
    21001 atctcagttc gtggctttgg tgaagcggaa tactggttct cactgatcaa agtcacgaca
    21061 gttattgtct ttatcatcgt tggcgtgctg atgattatcg gtatcttcaa aggcgcgcag
    21121 cctgcgggct ggagcaactg gacaatcggc gaagcgccgt ttgctggtgg ttttgcggcg
    21181 atgatcggcg tagctatgat tgtcggcttc tctttccagg gaaccgagct gatcggtatt
    21241 gctgcaggcg agtccgaaga tccggcgaaa aacattccac gcgcggtacg tcaggtgttc
    21301 tggcgaatcc tgttgttcta tgtgttcgcg atcctgatta tcagcctgat tattccgtac
    21361 accgatccga gcctgctgcg taacgatgtt aaagacatca gcgttagtcc gttcaccctg
    21421 gtgttccagc acgcgggtct gctctctgcg gcggcggtga tgaacgcagt tattctgacg
    21481 gcggtgctgt cagccggtaa ctccggtatg tatgcgtcta ctcgtatgct gtacaccctg
    21541 gcgtgtgacg gtaaagcgcc gcgcattttc gctaaactgt cgcgtggtgg cgtgccgcgt
    21601 aatgcgctgt atgcgacgac ggtgattgcc ggtctgtgct tcctgacctc catgtttggc
    21661 aaccagacgg tatacctgtg gctgctgaac acctccggga tgacgggttt tatcgcctgg
    21721 ctggggattg ccattagcca ctatcgcttc cgtcgcggtt acgtattgca gggacacgac
    21781 attaacgatc tgccgtaccg ttcaggtttc ttcccactgg ggccgatctt cgcattcatt
    21841 ctgtgtctga ttatcacttt gggccagaac tacgaagcgt tcctgaaaga taccattgac
    21901 tggggcggcg tagcggcaac gtatattggt atcccgctgt tcctgattat ttggttcggc
    21961 tacaagctga ttaaaggaac tcacttcgta cgctacagcg aaatgaagtt cccgcagaac
    22021 gataagaaat aagtttcctc ccttccttgc taagccctct caaccgagag ggctttttca
    22081 attccatttc cctgacaaat catgcggata taaaatttaa catttggatt gataattgtt
    22141 atcgtttgca ttatcgttac gccacaatca aaaaaggctg acaaatcaga ggctgttccg
    22201 gctttctggg atgatcacct gcataaaaaa taagtccacc gcgatgctgc cgtacgcaag
    22261 gggacgtgaa gaagatgtga gcgataaccc attttatttt cgtagttacc tcatggagat
    22321 atggaatgtt taggttgaac cctttcgtac gggtcgggct gtgtttgtcc gctatttctt
    22381 gtgcatggcc tgtgttagcg gtcgatgatg atggcgaaac gatggttgtc actgcatctt
    22441 ccgtggaaca aaatcttaaa gatgcaccag ccagtatcag cgtcattacc taggaagacc
    22501 tgcagcgaaa accggtacag aatctgaagg atgtcctcaa agaagtgcct ggcgtacaac
    22561 tgacgaacga aggggataac cgtaagggcg ttagtattcg tggtctggac agcagctaca
    22621 ccctgattct tgtcgacggt aaacgcgtta actcccgcaa tgccgtcttc cgccacaatg
    22681 atttcgatct gaactggatc ccggtcgatt ccatcgaacg tattgaagtg gttcgtggcc
    22741 cgatgtcgtc gctgtacggc tccgatgcgc tcggcggtgt agtgaatatc atcaccaaaa
    22801 aaatcggtca gaaatggtcg ggtaccgtta ccgtcgatac caccattcag gaacatcgcg
    22861 atcgcggtga tacctataac ggtcagttct ttaccagcgg accattaatt gacggcgtgc
    22921 tgggaatgaa agcttacggc agcctggcaa aacgtgaaaa ggatgacccg caaaactcaa
    22981 cgaccaccga taccggagaa acgccgcgta ttgaaggatt ctccagccgc gacggtaatg
    23041 tcgaatttgc ctggacaccg aatcaaaatc acgattttac tgccggatac ggtttcgacc
    23101 gtcaggatcg tgattccgac tcgctggaca aaaaccgcct ggaacgccag aactactccg
    23161 tcagccataa tgggcgttgg gattacggca ccagcgaact gaaatactac ggtgagaaag
    23221 tcgagaacaa aaaccctggc aacagcagcc cgataacttc cgaaagcaat acggtcgacg
    23281 gcaaatacac gttgccgctg acggcgatta atcagtttct cacggttggc ggtgaatggc
    23341 gtcacgacaa acttagcgat gcggtgaacc tgaccggggg aaccagctcc aaaacgtctg
    23401 ccagccagta cgcgctgttt gtggaagatg aatggcggat cttcgagccg ctggcgctga
    23461 cgaccggcgt gcgtatggac gatcacgaaa cctacggtga acactggagt ccgcgtgcct
    23521 acctggttta taacgccacc gacaccgtaa cggtgaaagg gggctgggcg acggcattta
    23581 aagcaccttc tctgttgcaa cttagccctg actggacgag caattcctgc cgtggcgcat
    23641 gtaagattgt gggtagcccg gatctgaaac cagaaaccag cgaaagttgg gagctggggc
    23701 tttactacat gggtgaagaa ggctggctgg aaggggttga atccagcgtt accgttttcc
    23761 gtaacgatgt gaaagatcgt atcagcatca gccgtacgtc tgacgtcaac gctgcaccgg
    23821 gctaccaaaa ctttgtcggt tttgagacgg gcgctaacgg acggcgcata ccggtattta
    23881 gctactacaa cgttaacaaa gctcgtattc agggcgtgga aaccgaactg aaaattccgt
    23941 tcaacgatga atggaaactg tcattcaact acacctacaa cgatggtcgt gatgtcagca
    24001 acggcgaaaa caaaccgcta tccgatctgc cgttccatac cgctaacggt acgctggact
    24061 ggaaaccgct ggcgctggaa gactggtcat tctatgtttc tggtcactat accgggcaga
    24121 aacgagccga cagcgcgacg gctaaaacac ctggcggtta taccatctgg aataccggcg
    24181 cggcctggca ggtgactaaa gacgtcaaac tgcgcgcagg cgtgctgaac cttggcgaca
    24241 aggatctcag ccgtgatgac tacagctata acgaagacgg acgtcgttac tttatggcag
    24301 tggattatcg cttctgatga gaagatgccc ggcgaaccgg gcggactttc acttcagtaa
    24361 atactgcgca tggaagcgca ggtgatcctc tataaaagag gcgatgaagt agtaactgtg
    24421 atcatatccc ggctgaatac gcagcgtcat cggccacgct ttctggcgcg cggcttctgc
    24481 cagaactgcg ggttgcaact gatcggcaag aaactgatca ttatcacctt gatcgataag
    24541 cgtcgggatc gcatcctgcg cgttactggc atacatcagc gcgcaactgt cccattccag
    24601 ccatgcattt ttgtcctcac ctaaatagcg gctaaacgct ttgatccccc acgggacgct
    24661 gcacggattc acaatgggcg caaaggcgga aacgctggtg tatttgcccg ggtttttcag
    24721 cgccataatc agcgcaccgt gaccacccat tgaatgaccg ctaatggcgc agcggtcgct
    24781 gacattaaat tgcgactgaa ccagcgccgg taattcatcg cgcagataat catacatccg
    24841 gtaatgcgtc gcccacggcg gttgcgtggc attaagataa aagcctgcgc cctggcctaa
    24901 atcgtagcca tcgtcgttgg caacctgttc gccgcgcggg ctggtgtctg gcatcaccag
    24961 tacaatcccc agttccgccg ctacccgctg ggcacccgcc ttggtggtga agttctcgtc
    25021 attgcaggtt aatccggaaa gccagtacag cactggtggt ggggtgtgat cacgaggtgg
    25081 tgggagaaag atactgaacg tcatcgggca gtttaaggta ctggagtcgt gtcgccagcg
    25141 ttgctgccag ccttcaaaac agcggtgctc ttcgagcatt tccatgcgtg gctccttgtt
    25201 gtgttgtttg caatttcttc atcataccgt ttattcaatg tgctgtgagt aactttcact
    25261 tccgtatttg cataacgatg ttttaacatc tgctgatgaa aggcagcggc aattacaata
    25321 attatcgctg tgaatactgg attatgtgcg ccgcctcacg cacaataatc aggctgtaaa
    25381 tcagcttaat aactttgccc ccacgcaggg cggaggcgtc acacctgcag gagaaatcat
    25441 aaatgccatc actcagtaaa gaagcggccc tggttcatga agcgttagtt gcgcgaggac
    25501 tggaaacacc gctgcgcccg cccgtgcatg aaatggataa cgaaacgcgc aaaagcctta
    25561 ttgctggtca tatgaccgaa atcatgcagc tgctgaatct cgacctggct gatgacagtt
    25621 tgatggaaac gccgcatcgc atcgctaaaa tgtatgtcga tgaaattttc tccggtctgg
    25681 attacgccaa cttcccgaaa atcaccctca ttgaaaacaa aatgaaggtc gatgaaatgg
    25741 tcaccgtgcg cgatatcact ctgaccagta cctgtgaaca ccattttgtt accatcgatg
    25801 gcaaagcgac ggtggcttat atcccgaaag attcggtgat cggtctgtca aaaattaacc
    25861 gtatcgtgca gttctttgcc cagcgtccgc aggtgcagga acgtctgacg cagcaaattc
    25921 ttattgcgct acaaacgctg ctgggcacca ataacgtggc tgtctcgatc gacgcggtgc
    25981 attactgcgt gaaggcgcgt ggcatccgcg atgcaaccag tgccacgaca acgacctctc
    26041 ttggtggatt gttcaaatcc agtcagaata cgcgccacga gtttctgcgc gctgtgcgtc
    26101 atcacaactg attaaaaggc aggaaccatg gagcgcaacg tcacgctcga ttttgttcgc
    26161 ggcgtcgcca ttctggggat cctgctatta aacatcagcg cctttgggct accaaaggcg
    26221 gcttatctca atcccgcctg gtacggcgct attacgccgc aggatgcatg gacctgggca
    26281 tttcttgatc tcgtcggcca ggtgaaattc ctcacgcttt ttgcgctgct gtttggtgcg
    26341 ggcctgcaaa tgttgctgcc ccgtggcaga cgctggatcc agtcgcggtt aacgctgtta
    26401 gtcttgctgg gttttattca cggtttattg ttctgggacg gcgatattct gctggcttac
    26461 gggctggtgg gcttaatctg ctggcggctg gtgcgcgatg cgccatcggt aaaaagcctg
    26521 tttaatacag gcgtcatgct ttatctggtg gggcttggcg ttttgctgtt attggggctg
    26581 atttccgaca gccagaccag ccgcgcctgg acgccggatg catcggctat tttgtatgaa
    26641 aaatactgga agcttcacgg cggcgttgaa gcgatcagta atcgtgccga tggtgttggc
    26701 aacagtttac tggcactggg cgcacagtat ggctggcaac tggcagggat gatgctcatt
    26761 ggtgcggcgc tgatgcgcag cggctggctg aaagggcagt tcagcttacg tcactatcgt
    26821 cgtactggtt ttgtgctggt ggcgattggg gtgaccatta accttcctgc catcgccctg
    26881 caatggcagc tggactgggc gtatcgctgg tgtgccttct tacttcaaat gccgcgggaa
    26941 ctgagtgcgc cgtttcaggc gattggctat gcgtcgctgt tttatggctt ctggccgcaa
    27001 ttgagccgct ttaagctggt gcttgcgatc gcctgcgtcg gacggatggc gctgaccaac
    27061 tatctattgc aaacgctgat ttgtaccatg cttttttacc acctcggttt gtttatgcat
    27121 tttgatcgcc tggagctgct ggcgtttgtt attccggtat ggctggcgaa tatcctcttc
    27181 tctgttatct ggctgcgtta cttccgccag gggccggtgg aatggctctg gcgtcagtta
    27241 actttgcgtg ctgccggaac ggcaatatct aaaacatcaa gataacgatc tggatcacat
    27301 tcgttaacaa aacggctgta accgtttcca ttgctgtgac tcgattcacg aagtcctgta
    27361 ttcagtgctg acaaaatagc cgccagcaag cagtcattta ctgcaatctc ataacaggta
    27421 gtgaatatga tcaccattcg tgatgtagcg cgtcaggctg gcgtctctgt ggcaacggtt
    27481 tcccgggtgc tcaataacag cacgctggtc agtgccgaca cgcgtgaagc agtaatgaaa
    27541 gccgtgagtg agctggatta tcggccaaat gccaatgccc aggcgctggc aactcaggtt
    27601 agcgatacca ttggcgtggt ggtgatggac gtttctgatg cttttttcgg cgcgctggta
    27661 aaagcggtgg atctggtcgc ccagcagcat cagaaatacg tgctaatcgg caatagctat
    27721 catgaagcgg aaaaagagcg tcacgccatt gaggtgttaa ttcgccagcg ttgtaatgcg
    27781 ttgattgttc actcaaaagc attgagtgac gatgaactgg cgcaatttat ggataacatt
    27841 cccggtatgg tgttaatcaa ccgcgttgtg ccggggtacg cccatcgttg cgtttgcctg
    27901 gataatctca gcggtgcccg aatggcgacg cgcatgttgc tgaataacgg tcatcaacgt
    27961 attggttatc tttcttccag ccacggcatt gaagatgacg ccatgcgtaa agcaggctgg
    28021 atgagtgcgt tgaaagagca ggatattatt ccgccggaaa gctggattgg cactggtacg
    28081 ccggacatgc cgggcggtga ggcggcgatg gttgaactgc tggggcgcaa tctacaactt
    28141 accgctgtat ttgcttataa cgacaatatg gccgctggcg cactgacagc attaaaagat
    28201 aatggcattg cgattccgtt acatctctca atcatcggtt tcgatgatat tcccatcgcc
    28261 cgttacaccg acccgcaatt aacgaccgtg cgttatccca ttgcttcaat ggctaaatta
    28321 gccaccgaac tggccttgca gggggcagca ggcaatattg atcctcgtgc cagccactgt
    28381 tttatgccga cgttagtgcg tcgtcattct gtcgcaacgc gccagaatgc ggcggcgatc
    28441 actaactcaa caaatcaggc gatgtaaccg ctttcaatct gtgagtgatt tcacagtatc
    28501 ttaacaatgt gatagctatg attgcaccgt tttaacgttg taacccgtat gtaacagtga
    28561 ataatcactt tcaccgaggt aatagcgtca taacaacaat taaagccgtt ttctggagcg
    28621 ttaccgggca tggaagaacg aattttaaaa agtgagcttc ggcgttcagt aacacttcat
    28681 taactctact gccccgccga gcatttatct caagcactac cctgcataag aaaaaccgga
    28741 gataccatga ataagaaggt gttaaccctg tctgctgtga tggccagcat gttattcggt
    28801 gccgctgcac acgctgctga tactcgcatt ggtgtaacaa tctataagta cgacgataac
    28861 tttatgtctg tagtgcgcaa ggctattgag caagatgcga aagccgcgcc agatgttcag
    28921 ctgctgatga atgattctca gaatgaccag tccaagcaga acgatcagat cgacgtattg
    28981 ctggcgaaag gggtgaaggc actggcaatc aacctggttg acccggcagc tgcgggtacg
    29041 gtgattgaga aagcgcgtgg gcaaaacgtg ccggtggttt tcttcaacaa agaaccgtct
    29101 cgtaaggcgc tggatagcta cgacaaagcc tactacgttg gcactgattc caaagagtcc
    29161 ggcattattc agggcgattt gattgctaaa cactgggcgg cgaatcaggg ttgggatctg
    29221 aacaaagacg gtcagattca attcgtattg ctgaaaggtg aaccgggcca tccggatgca
    29281 gaagcacgta ccacttacgt gattaaagag ctgaacgaca aaggcattaa aactgaacag
    29341 ttacagttag ataccgctat gtgggatacc gctcaggcga aagataagat ggacgcctgg
    29401 ctgtctggcc cgaacgccaa caaaatcgaa gtggttatcg ccaacaacga tgcgatggca
    29461 atgggcgcgg tagaagcact gaaagcacac aacaagtcca gcattccggt gtttggcgtc
    29521 gatgcgctgc cagaagcgct ggcgctggtg aaatccggtg cactggcggg cacagtactg
    29581 aacgatgcta acaaccaggc gaaagcgacc tttgatctgg cgaaaaacct ggccgatggc
    29641 aaaggtgcgg ctgatggcac caactggaaa atcgacaaca aagtggtccg cgtaccttat
    29701 gttggcgtag ataaagacaa cctggctgaa ttcagcaaga aataagactg attgggcgca
    29761 gtcgtttact gcgcccttat tgtatggcca acaaggtaaa attatatggt cagctcaacg
    29821 actccgtctt ccggggaata cttgttggaa atgagcggta tcaacaagtc ctttcctggt
    29881 gttaaggcac ttgataacgt taatttaaaa gtccggccac attctatcca tgcattaatg
    29941 ggggaaaacg gtgcaggaaa atcgacatta ttaaaatgcc tgtttggtat ttatcaaaaa
    30001 gactccggca ccattttatt ccagggtaaa gagatcgatt tccattccgc caaagaggca
    30061 ctggaaaatg gtatttcgat ggtacaccag gagttaaacc tggtattaca acgttcggtg
    30121 atggataaca tgtggctggg gcgatatccc accaaaggca tgtttgtcga tcaggacaaa
    30181 atgtaccgcg aaaccaaagc gatttttgat gaactggata ttgatatcga tccgcgtgcg
    30241 cgcgtcggca cattatccgt ttcgcaaatg cagatgatcg aaatcgccaa agcgttttcc
    30301 tataacgcga aaattgtgat tatggatgaa ccgacttctt cgttaaccga aaaagaggtc
    30361 aatcatctgt tcactattat tcgtaaatta aaagagcgcg gctgcggtat tgtttatatc
    30421 tcgcataaaa tggaagaaat cttccagtta tgtgatgaag ttaccgtatt gcgcgacggt
    30481 cagtggatcg ccaccgaacc gctggcagga ctgacgatgg acaagatcat cgccatgatg
    30541 gttgggcgtt ctcttaatca gcgttttcct gacaaagaaa acaagccggg cgaagtcatc
    30601 ctcgaggtgc gtaacctaac gtcactgcgc cagccgtcga ttcgcgatgt ctcgtttgat
    30661 ctgcataaag gggagatcct cggtattgcc gggctggtgg gggcgaaacg taccgatatt
    30721 gttgagacgt tatttggtat tcgcgagaaa tcggctggca ccattacgtt gcacggcaaa
    30781 cagatcaata accataatgc caacgaagcc ataaaccacg gatttgcact ggtaactgag
    30841 gagcgccgct caacgggaat ttatgcctat ctggatattg gttttaactc gttaatttcc
    30901 aatattcgca actacaaaaa taaagttggt ttactggata actcgcggat gaaaagcgat
    30961 acccagtggg tgattgattc gatgcgggta aaaacgccgg gtcatcggac gcaaattggt
    31021 tcgctctccg gtggtaatca gcaaaaggtg attattggtc gctggctact aacgcaacca
    31081 gaaatattaa tgctcgatga accgacgcgc ggtattgatg tcggggcgaa gtttgaaatt
    31141 tatcagttaa ttgccgaact ggcgaagaaa ggcaagggga ttattattat ctcctctgaa
    31201 atgcctgagt tgttagggat aacagaccgt attctggtca tgagcaatgg tctcgtttcc
    31261 ggaattgtcg atacaaaaac aacaacgcaa aacgaaattc tgcgtcttgc gtctttgcac
    31321 ctttaagatt aggggcttcc catgagtgcg ttaaataaga aaagttttct tacttacctg
    31381 aaagagggcg gtatttacgt cgttctttta gttttgctgg cgattattat tttccaggac
    31441 ccaacatttt taagtctgtt gaacttaagt aatattctca cccagtcatc ggtgcgtatt
    31501 attatcgcgc tcggtgtggc agggttaatt gtcacccagg ggaccgatct ttctgctggt
    31561 cgtcaggtag ggctggcggc agtggtggct gcgacattat tgcagtccat ggataacgcc
    31621 aacaaagtgt tcccggaaat ggcgacgatg ccgattgcgc tggttattct gattgtctgt
    31681 gccattggtg cggtgatcgg tttgatcaac ggtctgatta tcgcttatct caacgtgacg
    31741 ccgttcatta ccacgctcgg cacgatgatc atcgtctatg gcatcaactc gctctattac
    31801 gactttgtcg gggcgtcgcc aatttctggt tttgacagtg gcttctctac ctttgctcag
    31861 ggctttgtcg cgctggggag tttccgtctc tcttacatca ccttctacgc gttgattgcg
    31921 gtggcgttcg tctgggtgtt gtggaacaaa acccgcttcg gtaagaacat ttttgccatt
    31981 ggcggtaacc cggaagcggc aaaagtatct ggtgtcaacg tcggcctgaa cctgctgatg
    32041 atctacgcgt tgtctggcgt gttctatgcc tttggcggga tgttagaagc cggacgtatc
    32101 ggctctgcca ccaacaacct cggctttatg tatgagctgg atgctatcgc ggcgtgcgtg
    32161 gtaggcggtg tatcgttcag cggcggtgtg gggacggtga ttggcgtggt gaccggggta
    32221 attattttta ccgtcatcaa ctatggcctg acgtatatcg gcgtaaaccc atactggcag
    32281 tacatcatca aaggggcgat tattatcttc gccgtagcgc tggattcact gaaatacgcg
    32341 cgtaagaagt gatgtgattg tggatattgc cggatgcgct gcatccggca atcggtgcca
    32401 aatgccggat gcgtctctgg ttttacaacg ttaccgggtg ttctttctcg cctttcttaa
    32461 acttcacttc cccgagctca atacaaccca ccgggcagac gtgaccacaa agcagacaac
    32521 ccacacattt ctcggtatta caatgcggtg tgcgggtttt ctcgctccat tccatcgcct
    32581 ggtgaccgcc gtcgtaacag gaaatataac agcgtccaca gccaacacat ttatcaagat
    32641 tgatacgggg atagacaata taactgcggt ctaaatcttc cgccgggaca atattgttat
    32701 tcgccagacc taccatctcc tgcagcgaat caaatccctg atcggcgaga taatgcgaca
    32761 acccgcttgc catatcttcc actatccgat acccgtactg catgatgccg gtggtcacct
    32821 gtaacgttgc tgcgccgagc aataaaaact cagccgcatc ctcccaggtt tcaatgccgc
    32881 cgataccgct gattgggaaa tcgcgcagtt ctggatgggt gcgcatttgc tgaatgaagc
    32941 gcagggcgat cggttttacc gctttaccgg aatatccgga aatactcgat tttccgttaa
    33001 cgatcggcat accgattttc tgattaagat cgatattggt gatggattta acggtgttaa
    33061 tcgccgcaat gccatctgcg ccgccgcgct tcgccgccag cgccacttcg cacatatcac
    33121 cgatattcgg cgtcatcttc gccagcattg gcagcgtgga accccgtttc actgcccgac
    33181 aatatttttc taccagctcc gggctttgcc cgacatcgct acccatcgca tgagaagtca
    33241 tttgcggaca ggagaagtta cactcgatca tatccgcgcc agcttcttgc accaggcgcg
    33301 ccagctcctc ccattgctgc tcattttccc ccatgctcga agcgatcaat actttgtccg
    33361 ggtaatcttc cttcagctga cgcagggcgg ccagattctc ttccaacgga tgttcagcaa
    33421 tctgctccat atttttgaag ccgataaaac cggtatcttc tttcaccaga tgatcaaaac
    33481 gcggcgagac ttcgttggcg ataaaaaagc cgatcgtttt aaacaccacg ccgccccaac
    33541 ctgtgtcgta ggctttggca cacatctcat agcagttgcc tacaggcgaa gaagagaggc
    33601 agaacgggtt gggaaacttc acgccgcaaa aagtaatcga aagatctttc gttaacatga
    33661 gcaagctccc tctaaatagt gatgaatcgc ctcggcggct tctttcccgg ttttcacggc
    33721 atagaccacg gttttgtcac cctcaacaat atcgccagca gcaaagactt gcggatcgcg
    33781 ggtctgataa ttttgtgttt tgatggtgtt acgctgcggc tctaactccg caaaggcatc
    33841 cagtctggca tgctgaccga cggcgagaat aattttatct gccgccatcg tcagttcgcc
    33901 cggtaaccgt acatgcttaa acgtgacttt attgccttcc acggctactg gcgtgaatcc
    33961 atcgatgatc gaaacgccca gctcccgggc gctggcaaac tctttttcgc ttgccggaaa
    34021 ctcatctaac tcttcacgcg ctacgcaggt taccgcctga cagccgagaa ctttcagcgt
    34081 gctggctacg tccatcgcaa catcaccgcc gccgataatt aatgcgcttt gtggaatgct
    34141 gatatcgcct tgcgcctgtc gtgcacgttg caggaagtcc acggcaatct caacgttgct
    34201 atgctcaaac agcgataacc cggaaccgct cgataacccc acggtgacca gtaccgcgcg
    34261 gttttctgct ttcagctgct caagggtgag tgtgttaccg acttcgttgt tgcacttaat
    34321 ggtcacgccc attttttcaa tacgggcgat ctctgcatcc agcactgatt gcggtaaccg
    34381 gaattgcgga ataccgttac gcaaccagcc accggggtgc gcttctttct cataaatcgt
    34441 gacgtcataa ccctggtttg tcagtgttac actggcctgc aatccggcag gacctgcgcc
    34501 aataatcgcg actttgccga gcattttagt accgggctga taaattacca tcccagtttg
    34561 ttgttcaaaa tcagtaacaa aacgctgtaa gcgaccgata tcaatgggag tatcaacacc
    34621 ggcacgggta caactgcttt gacataattt ctccgtcggg cacactctgg cacaaacggc
    34681 accgagggca ttattttcgc gaattgtctc ggcagcgcct ttaaaattac gaaagtagat
    34741 tgagcgaata aatttccccg gatcggtctg tgccgggcaa gcctgactac agggagcgtc
    34801 atgacataat aaacagcgag aggcttcttt aatcgccagt aaaggcgtaa aagccggagt
    34861 gagttcatcc agataatttt gttgcggcat gtgatcttcc ttaatgctat gagtaattaa
    34921 ggcgaacgct aaatatactc gtcatacttc aagttgcatg tgctgcgtct gcgttcgctc
    34981 accccagtca cttacttatg taagctcctg gggactcact cacttgtcgc cttcctgcaa
    35041 ctcgaattat ttagagtata aatattgcgt gctgatatta aggctatgcg cgtggggaaa
    35101 tttatactgt gaaaggattc acaaatagct gcggttcaga atgttatatc cgcagcttat
    35161 tgagtgaagt aataattatt ctcaataatt aacagaaaag agaaaatatt aacgatagaa
    35221 aataagataa acaatcatgc cgatagataa aattccgaag ccgatatatg ccaccatata
    35281 agcgaaaaag agtccgggat tttttgctcg ccagacatac accggttccg gtgcattttt
    35341 aacccgttta tcaatcgcgc ctgcgcgcca gcctttccat gcttcgcgga aacaaaatat
    35401 cggaataagg aaaaaaacgg caacgacaaa aaactgctgt acatccataa ctactttcct
    35461 tgtttctttt gtaattcaag taactgttcg ggtgtgacgg cgggataccc ctgcgcatct
    35521 tccggcacct ggtgcatagc cggaataggg accagcggcc ctaaaaaacg cggttcacgt
    35581 ttaaaaatat aaaggtcagc cagcgcaccg aaacgggcgg caaattcgcg aatacgtact
    35641 gacagcatat ctttcggtga cggtacggca taacactgag cctgaatccc catatgcagc
    35701 gcaataaata atgcccgctc acagtggaaa cgttgggtga taataatgaa atcattagta
    35761 tcgaaaactt tgcgtgtacg cacgatggaa tccagcgtac gaaagcctgc gtaatcgaga
    35821 acaatatctg atgggtcgac accagcagcg attaaatctt tgcgcatggt catcggctca
    35881 ttataacttt gcaatgcgtt atcgccgctc agtaatagat aatttacctt accgctgtta
    35941 taggcattaa tcgctccttg aatgcggtag cgataatact gattaattac gccagtacga
    36001 taatattttg ctgttccgag caccacaccg acctggcggt aggggagatc ctgcaattcg
    36061 tcgtagatat aaggcgcggt tttccagctc atccagcgat cgaggccgag cacagtcaac
    36121 agcagcaagc cgatcaggac taacaggctg aggaacacgc gctttaacat gcaggagact
    36181 cgatctaagg aaaatttcca tcaggctact ttacccgctg gttttgcgca agaaacggca
    36241 aactgtttgc cgggaatttc gacaccagga gagggctggc aagccggaca aggcgttcac
    36301 gccgcatccg gcaccaggct taagcgagaa gcactcggtc gatactgtga cagccgagag
    36361 ctttcagtgt tgcggaggtg gcatcccact gaatcaacgg cgcatcggct ttttctgcca
    36421 gaaccgcgcg ctggatatcc gggtaatcat aacccttgag attcaataga attaacgctc
    36481 cctgcaacgg tggcagagtc gggttgaatg cggcgttttc agcgtagctg ccgctgaaaa
    36541 tacgaccgtc tttacattcc agcgtgacac cgcttggcga cttactgtaa ggcatgtgcg
    36601 aacggtttgc cgctgcaatc gccgcctgag aaagcgcatc accagtcagc gcatagccgt
    36661 gatcctgctc gtccatcagc agcgttttaa tctccagatc tttcggccca aaggcatccg
    36721 gcagatagtc acgcagcgcg tgtgcctcgc ggcccggcag atgaatacgc agatccagac
    36781 cgctgttcag ttcattcata aactgacgac agtgaccaca aggcgtgtag ttaacggtga
    36841 tggctgcaag cgctttttca ccactcaacc aggcgtggct gatcgcgctt tgttcggcat
    36901 gaacggtttg ctgcattgtc gcaccaataa attccatatt ggcaccgaaa taccaggttc
    36961 cgctcacacc gcgcgcaatt gcgccaacat taaaattcga caatggcgta cgcgcacagg
    37021 ccgccgccag cggaagtagt gcgaatgcca gcgcgtcttc gtccagcccc gttgcgctct
    37081 tcagcgatga gacttgctcc ccggtcaaca aagcggggaa gtacttgtct gccagaatag
    37141 gttccagtgc agattgcaag ttatccgcaa gttgggcaaa agcggtttga aaacgtggat
    37201 gcatggcgtt gcctcataat catgtaatgg gataacagtt tacgaacccg ttatggcttt
    37261 atatgtgatc tgaatctcat taattatgca acgcgtgaaa cttacatcaa aaatgcgcga
    37321 cgcatcgcaa attttagccc attactgcca gaataatcgg gaaaaggaac ggtgcgatca
    37381 gcgaagtaat tatcccgcat aacaccagcg ctagcgaact aaatgcacct tcctgataat
    37441 ccagctcggc gcagcgcgcc gtaccgaggg cgtgcgaggc agtccccatt gccagaccgc
    37501 gcgcagcttt ggtacgaata cgcatcgcat taagcaatgt atgaccaaat accgcgccga
    37561 ggatgccgac gaaaatcacg caaactgcgc tgattgccgg aataccgcca atactgccgc
    37621 caaccgccat tgcaataggc gtggtgactg atttcggcag gattgacgcg gcgatttccg
    37681 gtgaagcgcc catcaacaat gccacggaag tccccgtcac catcgcaacc acgctgccga
    37741 taaaacagat ggtgatgatc gatttccagc gcgcgcggat ctggtgtagc tgctcatata
    37801 aaggataagc caacgcgaca accgccggtt gcagcaaatc gtttagcact tcgctacctt
    37861 taaagtagct gtcgtaagag atgccggtga gcattaaaaa aggaatgatc accaccattg
    37921 ctaccagcaa cgggttaagc agggggaact tataccgtgc cgccagtttg cgggcggcaa
    37981 aaaagacaat caacgtcagc ggtaatgacc accagatatt cgccatcatt cttctgatcc
    38041 tttctgacct acgactttac gttcaccgtg taccagttgt gaactccagc tcaccaccag
    38101 aaaaaccacc agcgtactga ctgcacagga aaccactacc gggccaaact gtgcgcggag
    38161 caaatcaaaa tattgcatga cgcctacgcc aatcggcaca aacaatagcg ccatatagcg
    38221 aatcagtacg tagcaccccg gattgaccca ttttgccgga agaatttgca aggccagcag
    38281 gacaaacagg atcagcatac cgatgatgct gcccggaatg gttaccggta gcagggaagc
    38341 aatgaaaatg cctgcataca ggcaggcata aatcaggacg aaagcgcgta aatattgcca
    38401 gataatgttc agtgtcttac tcataatcaa caccccagat gagatggatt catcatacaa
    38461 ttaattcgta aaacgtgcta ccgatcacat taagaaatca gcgcttattt tttcgctccc
    38521 cggcattacg caatgcgcaa cgaactcagg catacatcaa gcatagagag ggattacagt
    38581 ttgcagcaat ccctctttgc agacctttaa tccgttgacc ataggcgcta acatggcgcc
    38641 tcattttttt gcgggtgaag atatgcgtgt gttactggct ccgatggagg gtgtgcttga
    38701 ctctctggtg cgtgaattgc tgaccgaagt taacgactac gatctgtgca tcaccgagtt
    38761 tgtccgcgtg gtggatcaac tgctgccggt aaaagtcttt catcgcattt gccctgagct
    38821 acaaaacgcc agccggacac catctggtac gctggtgcgc gtgcagttgt taggtcagtt
    38881 cccacaatgg ctggcagaga acgccgcccg tgcggtggag ttaggttcct ggggcgtgga
    38941 tctcaattgc ggctgcccgt cgaaaacggt taacggtagc ggcggcggcg cgacgttact
    39001 caaagatcct gaactcatct accagggtgc aaaagcgatg cgtgaagctg taccagcgca
    39061 tttgcccgtc agcgtaaaag tgcgtctggg ctgggacagc ggtgagaaga aatttgaaat
    39121 cgccgatgcg gttcaacagg ctggcgctac ggagctggtg gtgcatggac ggacaaaaga
    39181 gcagggttac cgcgcggagc atattgactg gcaggcgatt ggcgagattc gccagcggct
    39241 gaatattccg gtgattgcca acggcgaaat ctgggactgg cagagcgcgc aacaatgcat
    39301 tgcgatcagc ggctgcgacg cagtgatgat tggtcgcggg gcgctcaata ttcccaacct
    39361 gagccgggta gtaaaatata acgaaccgcg aatgccgtgg ccggaggtgg ttgctttgct
    39421 gcaaaaatat acccgtctgg aaaagcaggg cgataccggg ttatatcacg ttgcgcggat
    39481 taaacagtgg ttgagttatt tgcgtaaaga atacgatgaa gcaacggaat tatttcagca
    39541 tgttcgggtg ttgaataatt cccctgatat tgcaagggct attcaggcaa ttgatatcga
    39601 gaaactctaa aaaaccatag tgatgttttt tgacaatgtg ttagctcttt atcaataggc
    39661 cgcacggttg tttggtggcg tcaaaattgc cgaggcgttc cctgaaggcc ggggcagccc
    39721 acatggatgt gggctgaggg cgcgttttac agggatgtta cctcgcgccc gacccgatag
    39781 ccgtaaggga taagccgagg gcaccgcgca gcggcgattt tgttcgccag agcccggggg
    39841 tgcagggggc ggcggcgatt ggccgccctg cgcgctcctt gcgccagtga ttatatgaag
    39901 caatgcacat gacaggagcg caacaggatg agagactgaa atattctcgt tattttcgga
    39961 cctgatgaca ttaattaatt ttaaaatatc atcttaaata cgccagtcac caccagtagc
    40021 ccaatcagga aaataatcaa tacagcccag agtataattt tcattggcgt tcctttattt
    40081 gtgagtgctg aataagtata ggaaaaaacg aagccctttg ccgttatcca taaacgatta
    40141 agataaatcg gaagatatca tttacttagt ctatattttt ctggatatgt cgttttattg
    40201 ctactgatgt attgccacgc gaaactaaaa aatatatcgc aacacacggt aatctccgcg
    40261 caccttttct tacctgatta ttcccccatg aatcgtgatt ccttttatcc agccatcgcc
    40321 tgttttccgc tgttactgat gctggccggg tgtgcaccta tgcatgaaac ccgccaggcg
    40381 ttaagccagc aaacgcctgc tgcacaagtt gacaccgcat tacccacggc gctgaaaaat
    40441 ggttggccag acagccaatg gtggctggag tatcacgata atcaactcac ttccttaatt
    40501 aacaatgcac tacagaacgc accggatatg caggtggctg aacagcgcat tcagctagct
    40561 gaagcccagg cgaaggcaat tgctactcag gatggtccgc agatcgactt ttctgcggat
    40621 atggagcggc aaaaaatgtc ggcagaaggc ttaatggggc cgtttgctct gaacgatccg
    40681 gccgcaggta cgaccggtcc gtggtacacc aacggaactt ttggcttaac ggcgggctgg
    40741 catctcgata tctggggaaa gaatcgggcg gaggttactg cccgcctggg tacggttaaa
    40801 gcacgggcgg cggaacgcga gcaaacccgc caattgctgg ctggcagcgt agcccgcctg
    40861 tactgggagt ggcaaaccca ggcggcgtta aacacggtct tgcagcaaat agaaaaagag
    40921 cagaacacca ttatcgcgac cgatcgccag ctatatcaga acgggattac ttcttcagtt
    40981 gaaggt
//
LOCUS       DAMZET010000021        39193 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.021, whole genome shotgun sequence.
ACCESSION   DAMZET010000021 DAMZET010000000
VERSION     DAMZET010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.021"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..659
                     /gene="yieH"
                     /locus_tag="POB34_001239"
     CDS             <1..659
                     /gene="yieH"
                     /locus_tag="POB34_001239"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HDI5918224.1"
                     /translation="IEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFKRFK
                     GVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITAPMC
                     VVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
                     LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
                     "
     gene            726..1199
                     /locus_tag="POB34_001240"
     CDS             726..1199
                     /locus_tag="POB34_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918225.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLRRIQ
                     VLPLFLLLSITTGVIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELYENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            1248..1835
                     /locus_tag="POB34_001241"
     CDS             1248..1835
                     /locus_tag="POB34_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HDI5918226.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKRGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(1897..2619)
                     /locus_tag="POB34_001242"
     CDS             complement(1897..2619)
                     /locus_tag="POB34_001242"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDI5918227.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(2634..3803)
                     /locus_tag="POB34_001243"
     CDS             complement(2634..3803)
                     /locus_tag="POB34_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDI5918228.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(3830..5446)
                     /gene="bglH"
                     /locus_tag="POB34_001244"
     CDS             complement(3830..5446)
                     /gene="bglH"
                     /locus_tag="POB34_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HDI5918229.1"
                     /translation="MFRRNLITSAVLLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(5532..6926)
                     /gene="bglB"
                     /locus_tag="POB34_001245"
     CDS             complement(5532..6926)
                     /gene="bglB"
                     /locus_tag="POB34_001245"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HDI5918230.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(6945..8822)
                     /gene="bglF"
                     /locus_tag="POB34_001246"
     CDS             complement(6945..8822)
                     /gene="bglF"
                     /locus_tag="POB34_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HDI5918231.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLDEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTSLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(8954..9790)
                     /gene="bglG"
                     /locus_tag="POB34_001247"
     CDS             complement(8954..9790)
                     /gene="bglG"
                     /locus_tag="POB34_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HDI5918232.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILG
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVHK
                     EH"
     gene            complement(10076..10801)
                     /gene="phoU"
                     /locus_tag="POB34_001248"
     CDS             complement(10076..10801)
                     /gene="phoU"
                     /locus_tag="POB34_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDI5918233.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(10816..11589)
                     /gene="pstB"
                     /locus_tag="POB34_001249"
     CDS             complement(10816..11589)
                     /gene="pstB"
                     /locus_tag="POB34_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HDI5918234.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(11680..12570)
                     /gene="pstA"
                     /locus_tag="POB34_001250"
     CDS             complement(11680..12570)
                     /gene="pstA"
                     /locus_tag="POB34_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDI5918235.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(12570..13529)
                     /gene="pstC"
                     /locus_tag="POB34_001251"
     CDS             complement(12570..13529)
                     /gene="pstC"
                     /locus_tag="POB34_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HDI5918236.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(13616..14656)
                     /gene="pstS"
                     /locus_tag="POB34_001252"
     CDS             complement(13616..14656)
                     /gene="pstS"
                     /locus_tag="POB34_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDI5918237.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(14904..15977)
                     /locus_tag="POB34_001253"
     CDS             complement(14904..15977)
                     /locus_tag="POB34_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI5918238.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(15988..18510)
                     /locus_tag="POB34_001254"
     CDS             complement(15988..18510)
                     /locus_tag="POB34_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDI5918239.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            complement(18535..19266)
                     /locus_tag="POB34_001255"
     CDS             complement(18535..19266)
                     /locus_tag="POB34_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDI5918240.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            complement(19314..19886)
                     /locus_tag="POB34_001256"
     CDS             complement(19314..19886)
                     /locus_tag="POB34_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HDI5918241.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
     gene            complement(20188..22017)
                     /gene="glmS"
                     /locus_tag="POB34_001257"
     CDS             complement(20188..22017)
                     /gene="glmS"
                     /locus_tag="POB34_001257"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDI5918242.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(22179..23549)
                     /gene="glmU"
                     /locus_tag="POB34_001258"
     CDS             complement(22179..23549)
                     /gene="glmU"
                     /locus_tag="POB34_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDI5918243.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(23900..24319)
                     /gene="atpC"
                     /locus_tag="POB34_001259"
     CDS             complement(23900..24319)
                     /gene="atpC"
                     /locus_tag="POB34_001259"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDI5918244.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(24340..25722)
                     /gene="atpD"
                     /locus_tag="POB34_001260"
     CDS             complement(24340..25722)
                     /gene="atpD"
                     /locus_tag="POB34_001260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDI5918245.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(25749..26612)
                     /gene="atpG"
                     /locus_tag="POB34_001261"
     CDS             complement(25749..26612)
                     /gene="atpG"
                     /locus_tag="POB34_001261"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDI5918246.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(26663..28204)
                     /gene="atpA"
                     /locus_tag="POB34_001262"
     CDS             complement(26663..28204)
                     /gene="atpA"
                     /locus_tag="POB34_001262"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDI5918247.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(28217..28750)
                     /gene="atpH"
                     /locus_tag="POB34_001263"
     CDS             complement(28217..28750)
                     /gene="atpH"
                     /locus_tag="POB34_001263"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDI5918248.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(28765..29235)
                     /gene="atpF"
                     /locus_tag="POB34_001264"
     CDS             complement(28765..29235)
                     /gene="atpF"
                     /locus_tag="POB34_001264"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDI5918249.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(29297..29536)
                     /gene="atpE"
                     /locus_tag="POB34_001265"
     CDS             complement(29297..29536)
                     /gene="atpE"
                     /locus_tag="POB34_001265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDI5918250.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(29583..30398)
                     /gene="atpB"
                     /locus_tag="POB34_001266"
     CDS             complement(29583..30398)
                     /gene="atpB"
                     /locus_tag="POB34_001266"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDI5918251.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(30407..30787)
                     /gene="atpI"
                     /locus_tag="POB34_001267"
     CDS             complement(30407..30787)
                     /gene="atpI"
                     /locus_tag="POB34_001267"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HDI5918252.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLQAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(31404..32027)
                     /gene="rsmG"
                     /locus_tag="POB34_001268"
     CDS             complement(31404..32027)
                     /gene="rsmG"
                     /locus_tag="POB34_001268"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDI5918253.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(32091..33980)
                     /gene="mnmG"
                     /locus_tag="POB34_001269"
     CDS             complement(32091..33980)
                     /gene="mnmG"
                     /locus_tag="POB34_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDI5918254.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(34359..34802)
                     /gene="mioC"
                     /locus_tag="POB34_001270"
     CDS             complement(34359..34802)
                     /gene="mioC"
                     /locus_tag="POB34_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HDI5918255.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKDSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(34892..35350)
                     /gene="asnC"
                     /locus_tag="POB34_001271"
     CDS             complement(34892..35350)
                     /gene="asnC"
                     /locus_tag="POB34_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HDI5918256.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            35502..36494
                     /gene="asnA"
                     /locus_tag="POB34_001272"
     CDS             35502..36494
                     /gene="asnA"
                     /locus_tag="POB34_001272"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDI5918257.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKVLPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(36499..37950)
                     /gene="viaA"
                     /locus_tag="POB34_001273"
     CDS             complement(36499..37950)
                     /gene="viaA"
                     /locus_tag="POB34_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HDI5918258.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(37944..>39193)
                     /gene="ravA"
                     /locus_tag="POB34_001274"
     CDS             complement(37944..>39193)
                     /gene="ravA"
                     /locus_tag="POB34_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HDI5918259.1"
                     /translation="GPLSIQALKDEGRYERLTSGYLPEAEIVFLDEIWKAGPAILNTL
                     LTAINERQFRNGAHVEKIPMRLLVAASNELPEADSSLEALYDRMLIRLWLDKVQDKAN
                     FRSMLTSQQDENDNPVPDALQVTDEEYERWQKEIGEITLPDHVFELIFMLRQQLDKLP
                     DAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVDLILLKDCLWYDAQSLNLIQQQIDVL
                     MTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKTALTVIRLGGIFSRRQQYQLPVNVTA
                     STLTLLLQKPLKLHDMEVVHISFERSALEQWLSKGGEIRGKLNGIGFAQKLNLEVDSA
                     QHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQLEELESDWRKQHALFSEQQKCLFIPG
                     DWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         9797 a        10067 c         9316 g        10013 t
ORIGIN      
        1 agatagaagc ggtatttttc gactgcgacg gtacgctggt cgacagtgaa gtcatttgct
       61 cccgcgcata tgtaacgatg tttcaggaat ttggtattac gctcgatcct gaagaggtat
      121 tcaaacgttt caaaggtgta aaactgtacg aaattatcga tattgtttcc cttgaacatg
      181 gtgttacgtt agcgaaaaca gaagctgaac acgtttaccg tgcagaagtc gctcggctgt
      241 tcgattcaga actggaagcc atcgaagggg ctggagcgct cctgtcagcg atcactgcgc
      301 caatgtgtgt ggtatctaac ggcccaaata acaaaatgca gcattctatg ggcaagctga
      361 atatgttgca ctacttcccg gataaactgt tcagcggcta cgatattcag cgctggaagc
      421 cagacccggc gttaatgttc catgcggcaa aagcgatgaa tgtaaatgta gaaaactgca
      481 ttctggttga tgactcagtt gccggtgcgc aatctggtat cgacgcaggt atggaagtgt
      541 tctacttctg cgccgacccg cacaataagc cgatcgttca cccgaaagtc accaccttta
      601 cccatctttc gcagttaccg gaactgtgga aagcgcgtgg ttgggatatt acggcatagt
      661 tcttcacact cccttcactt accccgctta aattggcgct caaaggtaag taaagggagt
      721 ttgatatgtc tgtttcacgt cgggtaatac atcacggact ttattttgcg gttttaggac
      781 cgttaattgg tgttctgttt cttgtcctct acatattctt cgcaaaagaa ccgctggttc
      841 ttttgcgaag aatacaggta cttccattgt ttctcttatt gtcgattact acgggagtta
      901 ttcctgcatt gttaaccggc gtgatggtcg cctgtctgcc ggaaaagatc gggtcacaga
      961 aaaattatcg ttgtctggct ggtggcatag gtggcgtcgt tatcaccgag atctattgtg
     1021 cagttattgt acatattaag ggcatggctt cctcggagtt gtatgaaaac attctttctg
     1081 gtgacagtct cgttgtccgc atcattcctg cattgctggc aggtgtggtg atgagcagaa
     1141 tcattacccg tctgcccgga ttggatattt catgtcctga aacagactct ttaagttaag
     1201 cgggatactt tatctttggg ctactcaaaa gcagacagga tgttactatg actcaaaata
     1261 tcaggccgtt accccaattc aaatatcatc ccaagccact ggaaacaggc gcatttgaac
     1321 aggataaaac cgtagagtgc gattgctgtg aacaacagac gtcagtttat tactcgggtc
     1381 ccttttattg cgttgatgaa gttgaacatc tctgtccgtg gtgtattgcg gacggttctg
     1441 ctgctgaaaa gtttgcaggt agttttcagg atgatgccag catagaaggt gttgaatttg
     1501 agtatgatga agaggacgaa tttgccggta ttaagaacac atatcctgat gaaatgctga
     1561 aagagttggt tgaacgcacg ccaggttatc atggatggca acaggaattc tggcttgcgc
     1621 attgtggcga tttctgtgct tttatcggct atgtgggctg gaatgatata aaagatcgcc
     1681 tcgatgaatt tgccaacctt gaagaagatt gtgagaattt cggtattaga aattctgatc
     1741 tagctaaatg cctgcaaaag cgtggtgatt gtcagggtta tctcttccgc tgtctccact
     1801 gcggcaagct gagactgtgg ggtgattttt cgtagttatt taaataatga gaacaggccg
     1861 gagcgtaatt catacatccg gccttatttc ttcagcttaa ccgagcgcta attctgcggc
     1921 agcggcttta tcagcgatta ccatcagcga aggatgcagc tgcaacacgg aagctggaac
     1981 atcttcggta accggacctt gcagcacatt tttcagtgcc tgcgctttac ctgcgccact
     2041 gacaattatc agcaagtttt tcgccgccat gatgcttttc ggccccatcg tcacgtagct
     2101 gtcaggcact aacgaaaagt cgccgcccaa ctcaccatgc gccacaatat caaccatctc
     2161 tccctggatc gggaactcca cggtttgctc atggaaatgg gtcgtattcg gcaaattgcc
     2221 acaaaaatga ccatctgcac ctaatcccag caccaccaaa tccaggccgc cttcacgcgc
     2281 cagtttctga tcatgctcgc ggtagttatc aatagtgagc ttctggatat tctcttcttt
     2341 gatccccgca ggggtgaaaa acagattacg cagattggta atcgttacac cttctccctc
     2401 tttgccgcga aagggaattt catcgaaatt atagaaatag cagttatcat accagggctt
     2461 acctttaacc agggtggtga ggtattcata catgcctttg ggcgtgctac cggcggtaat
     2521 tgccaggtta acacgacgcg tcttcgacat ataaccaagc agatggtgtg ccgcgacacg
     2581 gctcatttcc tggtaatctt cggtaatgat taatttcatc ttagtgcctt attttattta
     2641 aataatttct gtacgaaggc tgcataggca ggccgccaga catccatttc atggttcagg
     2701 ccgggatatt cctggtaatc aaacttaatt tttttctgct caagctcagt tttcagcccg
     2761 gcgatatcct tgccggttac gacatcttta tcaccaacga ccacagtaaa attacgtagt
     2821 tgctggttaa tagctgccgg atcgttcagc cgggccgcga caccttcatc cggtacggtt
     2881 gtcgtggtaa caccactgaa tgtggccagc cagccaaagc tttccagatg attcattccg
     2941 gaaaccagcg cctggtaccc gccttgtgaa agccctgcca gcgcgcgacc atcggcatct
     3001 ttacggacat taaaacgctt gctaatcagc gggataatat cgttcatcag ttcgcgatct
     3061 gccgctttag cattcagcgg ataaaagact ttacgtcttt cctgaggcac gaaatcttcg
     3121 ggaataatgc ccttcgcatc ggtttcggta tccgggatca ccaccagcat cggtttaatt
     3181 ttcccttcag ccagcaggtt atccatgatt tgcgggatac gcccctgatc gatagcggaa
     3241 cgtccggtat caccaaaacc gtgatagaaa tagagcactg gcaaaggctc gcccatgccg
     3301 gtgtatcctg gcggggtcca gacatacatc tgacgttcag attgcaatgc gttggagtgg
     3361 taagttatgg cgatcaaatc accatgcgca acagaacgcg tatccagata actgcctggc
     3421 accagaatca tactggagtt aacctggcgc tgaggcttag tcattgcggt gcctgtatca
     3481 atgctgcgta caccatcaac attgaaaaaa tactcgtaca ggttgccttt caggataggt
     3541 gtgcgccacg accagactcc tgcttcgtct ttggtcatcg ggtgaatatt gtccggaacc
     3601 ggaacaccca ccactacaga gacatttttt gccccagggg caaagtagcg aaaggtcacg
     3661 ctgttatcgg cattgacctg agtcacatac tgtttcaccg ggatagtggg tgccgggctg
     3721 gctggcatat ccgctgctat ggcccactga gcagaaatac cgctggcaat agccagcgtt
     3781 aaagcagcaa tttttatatt catcgtaata ttcctttcat taagttccat taccaccaga
     3841 tttcagcctg ggcaccgaca gcgaactggt cgtctttatt gtcttcgaag gtgaagccat
     3901 ccagttcgtt ttccagggct ttgatatacg tggcgtagaa acgaatttcc ggacgcgagg
     3961 tcaacatact ggtattgact ttaaaggtat ggaagagcgt ggttttataa ccagactcat
     4021 taaatttatt actattcgca tctttgtttt gctgggtgaa atagcccagt tcaacacctg
     4081 tctggttata ttggtcccaa atataggccg ggcgaacaac cgcacgaata gattcgaaat
     4141 cagagtgtgc gcctgtttcg tagctataaa tatcgttacc gaaggagtaa acaatcgcgt
     4201 tagccacaat aaaatgatcg ccgatatagg cttcgccctg tgaagtcaga cgaactgctg
     4261 ttccgccggt gtgatcacca taataacgac cattaaaggt ggtaaatgga cttgcgccag
     4321 cataacggcc aaagttactg gcgatagagt tattcgcaac caggaaggag aattcgttga
     4381 agccaccttt atcaaatttc tgcgttaaag atgtgccgaa catccaggta tctttccagt
     4441 cataataccc gttattatcc tgattatctt tttccgatgc gctttcgttt gccgtgacat
     4501 aacgaccact caccattaag gtcgctttat cccataacgg gatatcttta tagcgtaaat
     4561 caatagtatt ggtattaatc tgctgtttgt tttgcaggct gcgatcgtaa tcatcgacat
     4621 cttcgcgaac cagcgcgata tcaattttac ccggaccgac tttccagttt tccagaccta
     4681 cacccgctgc ggcatcagta cgctgcgttt tccagtccag catctggatt tcaattttcg
     4741 gcgcaccgtg tttacccacc cagaaatcag cctctggcgc aaagggcagg aaacctttgg
     4801 tggtaacgta catatcggag aactgcataa agttctcgcc accggcgtta tcgccaaacc
     4861 agccggtaga gtactgctga ccaacgttac catccatcat cacaacggca tcaacccgtt
     4921 tgccgttttc gttgtagaca cgttgtttta actgcaaatc aaaccagccg gagtattcgt
     4981 taccaaagcg gcccagagaa ccaatcgccc atgatttagg tgaaccatga gaggcggtcc
     5041 cccaaccaga acggtaatag ccgttgtaac taaagccaat ttcatctttc acaaacttac
     5101 tgaaatcttt cagagtcatg gaagaataaa tcgagcctgt ctcactggca tttttctctt
     5161 catttttgac cagtacagca tcaggttttg cggcactact ggtcgggaac ggattggcgg
     5221 catacccttg atcattcgta cttacgctac gattcaccgt cgctggcgta tattttttct
     5281 tctcttcatc tttatacttt ttgagttcgc tttgcgtttc tcttaacgct ttttctaata
     5341 actcaaggcg ttgttccacc gttaatgatt cagccaatga ttgtgcagaa aaggctaacg
     5401 gtgccattaa taagacggca gaggtaataa gatttcgtct aaacataata tccctttatg
     5461 gtgcaaagaa agaattaacg tatcgcatcc agactattct gaatgcgacg ataattaagg
     5521 tgctttattg gttatttttt taatgacagc ccccgcgttt tgatcacttc tgcataccat
     5581 ccgaagcttt tcttacgcgt tcttgtgagg cttccttcgc cattatcatc acgatccaca
     5641 taaataaagc cgtagcgctt agacatttgt gaatgagatg cactgactaa atcaattggc
     5701 ccccaactgg tgtaccccat aatatccaca ccatcggcaa tcgcttcatt tacctgtacc
     5761 aggtgatcgt ttaaataggc aattcgataa tcgtcctgta tcgaaccatc cgcttcaacg
     5821 ctgtcttttg cgcctaatcc gttctcgaca ataaataacg gtttttgata acgatcccaa
     5881 agcgtattta acagaacccg taatccaacc ggatcaattt gccaccccca ctctgaactt
     5941 ttcagatgcg gattggggat catattcagt atgttgccct gcgcattttt attaatgctt
     6001 tcgtcgtggg aaacacaacc agtcatgtaa taactaaaag agatgaaatc gacggtatgt
     6061 tttaaatctt ctgcgtcact ttcagtcatc tcaatggtga tattgtggtc gcggaagaaa
     6121 cgctgcatat agccgggata ctggccacgc gcctgaacat caccaaagaa catccagcgc
     6181 cggttctctt ccatggcctg caacatatcc tgtggctggc aggtgagggg gtaaaccagc
     6241 ccaccgagaa gcatattgcc gattttcgct tcggggagca ggctatgaca ggctttaact
     6301 gcccgcgcac tggcaaccag ttgatggtgg atagcctgat aaacttccgc ctcgccactc
     6361 tcttctgcca gccccacgcc cgtgaatggc gcgtgtaacg acatgttgat ttcattaaac
     6421 gtcagccata acgccacttt atgttggtag cgagtaaaga ccgtgcgggc gtaatgctcg
     6481 aagtgatcga tgaccgctcg attagcccaa ccgccgtagt ttttcaccag cccatatggc
     6541 atttcgtaat gggataacgt taccagcggc ttgatccccg cctgcgccat ttcatcaaac
     6601 agccgatcgt aaaacgctaa ccccgcttca ttcggttcga cttcgtcgcc ctgagggaaa
     6661 attcgcgccc aggcaatgga aatacgcaga caggtgaagc ccatctcggc aaataacgcg
     6721 atatcttccg ggtaacggtg ataaaaatcg atggcgacat ctttgatatt ctctttcccc
     6781 aggatgcgcg gttccatttt tcccattacg ccatgaggct gtaaatctga ggtcgagatc
     6841 cctttgccat cttcctgcca ggcaccttcc acctgattgg cagctgttgc gccaccccaa
     6901 agaaatgttt ctggaaatgc tttcataatt aactcctttt atcgttagcg aatgatggat
     6961 aacagcggtt cacctgcgct tatctgcgcc gtgccgtggg gtaatacgtc cgtaaaatca
     7021 tcgctattac tgattaatac cggcgtcgtc agatcaaatc cggcctcgcg aatagcaggg
     7081 atatcaaaag aaatcagccg atcgcctgta ttgaccttgt cacccacgtt gacgtgagcg
     7141 gaaaagaatt tgccgtccag ttttacggtg tcgataccga catgaatcag gatctccaca
     7201 ccatcatctg actcaatgcc aatggcgtgt aatgtggcga acaacgaagc aattcgaccc
     7261 gcaaccggag aacgcacttc accaaccgag ggcagaatgg cgataccttt acccaacagg
     7321 ccactggcaa acgtggtatc agcgacgtga atgagcggca caatctctcc cgtcatcggt
     7381 gaacagatac cgccctgctc aggtggtgta ataacctctg gtgttttctc ttgcggggca
     7441 ccctgcgctg gctgacgttt agcggtgatg aaatgaagca tcaccgtacc gacaaatgcg
     7501 caaccgatgg caatgacacc gccaataacg ctggcccaga cggtgaaatc aattcccgtt
     7561 gacgggatgg tttgcatgaa ggtgaaaata cttggcaaac caaaggagta gactttcgtt
     7621 tgcgcgtagc caatgatggt ggcccccaaa gccccactga tacaggcgat aacaaagggg
     7681 tacttacgcg gcaggttgac gccatatacc gctggttcgg tgataccaaa cagactcgtc
     7741 aacgccgctg atcccgccac cacttttttc tgcgcatcgc gttcgcagag gaagacgccg
     7801 agcgccgccc cgacctgcgc cataatggcg ggcattaaca gcgggatcat ggtgtcgtag
     7861 cccagcacgg tgaagttatt gatacacagc ggcaccaggc cccagtgcag tccgaacatg
     7921 acgaagattt gccagaagcc gcccattacc gcgcccgcaa atgcaggaac cgcctgataa
     7981 agccagagat aaccggcggc aatcagttcg cttatccagg ttgatagcgg ccccaccagc
     8041 agaaaggtga cgggtgtgat aaccatcaga catagcaatg gtgtgaagaa atttttgatt
     8101 gccgacggta accacgcatt aagtcggcgt tccagaatgc tgcacaacca ggcagaaaaa
     8161 ataatgggaa taaccgatga cgagtaattc aacaatgtga ccggaatacc caggaaatcc
     8221 agccccagcg catccgcttt ttgcccgttc tcgaaagcag tcagaattaa tggatgcact
     8281 aacgctccac caatcaccat ggcagtaaat ggattaccgc cgaagcgttt ccccgcggtg
     8341 tatcccagga ttatcgggaa gaaccaaaac aaggcatcac tggcgctgaa taaaattaaa
     8401 taagtaccac tttgttcggt cgtccactga aaagtgagcg ccagagccag catacctttc
     8461 aggatcccgg ttgccgccat caaaccgatc agaggcgtaa aaatacctga aataacataa
     8521 acaaagcggt ttagcagatt acctttatca tcattttccg gtgcctgttg cgctttttcg
     8581 tcaaggcctg ccacactgtt aaccgccagg aagacatcgg ccacatggtt acctatgacc
     8641 acctgaaact ggccaccgct ttccaccacc ataataatac cgggggtctt tttcagtacc
     8701 tctgcttgcg ctttgctttc atcctttaac ttaaaacgta atcgcgttgc gcaatgcatc
     8761 agactcacaa tgttatctgc gcccccgact cctgcgacta tttttctggc taactccgtc
     8821 ataacttgcc ctctaccgct ttgcggcaaa actcaaaaaa aaacctgaaa aaaacggcct
     8881 gacgtgaatc aagcaatttt ttcaggtttt gcccgcttag tgcggtaaca atcctttact
     8941 cagtaataat atttcagtgt tctttgtgca cgcgctctat atttatggct aaaaacataa
     9001 tctctgcggg tgagatttta cgttgatact gcaaaccaat aaaaatggcg atccgctccg
     9061 cacattgcca tgcttgcggg taattttgtt ttactgcttg ttgtaatgac tcatcactat
     9121 cgttaattga agcatgtcca agaatacgcc aggataaaaa cttcagatgc gtaaccagtc
     9181 gctgataact caagctttct tcctggtaat taaggctgaa ctgaaatttt attaattgca
     9241 gcatttcgcg cattaattgc gtgacacctg caacatcctc catatttccg ctcatttggg
     9301 cactgaccag atgcatggca ataaagccca cttcatcttt cggtaactgc acgcccaacc
     9361 gtttatcaat aatggttaac gcttcttccc ctagctggaa ctctttcggg taaagccgct
     9421 ggatatccca cagcagcggg ttgggcagta acacgttttg ctgaaagcgt ttaatcgcaa
     9481 actggcaatg gtcagttagc gagatataaa tactgtcctg taattttccc aagcgctcct
     9541 gcgctaaaga gataatacga tcacaggttg ccatcacctc aagaggaata tgacttaaga
     9601 gttcgcttaa tcgcccgttc agttcatgac tgctcaaggc atactctttt tctattccac
     9661 ttgagttaat tctttcgcca gcgcgttttt gaaagccaat tcctcgcccc atgacgactt
     9721 tttcccgctg ttgatcatca ataaccacca caacattatt gttgagaatt ttggtgattt
     9781 gcatgttcat agcaaggacc ttttttataa acaaaaaaaa ccgacttcac cagtattctc
     9841 tggttatgtc aggttttgcc tgcgaatgca gtaacaatcc agtcatttat taatggtttt
     9901 tataacgaac atccaggttc ggaaattaat ttaattgcgt gcagagaaaa tagcaatgcg
     9961 ctattgataa aaatatgacc atgctcgcag ttattaactt tgtgtaattt taggaattta
    10021 taaagttata tataacaaat cccaataatt aagttattgg gatttgtctg gtgaattatt
    10081 tgtcgctatc ttttcccgcc agcagtttat ccagctcatc accgcctaca tgacggaaat
    10141 cctgcccctt cacgtagtag aagataaact cacaaatatt ctggcagcgg tcgccaatac
    10201 gttcgataga acgcgcgcag aacagcgcag taagtacgct cggaatggta cgcgaatctt
    10261 ccatcatgta ggtcatcagt tgacgaacaa taccttcgta ttcctgatcg acttttttat
    10321 cttcacgata aatacgtacc gcttcgtcaa tgtccatccg cgcgaacgcg tccagcacgt
    10381 cgtgcagcat ctggatggta tgacggccca gcgactccag acttaccagc aacggctgat
    10441 gctgctggga gaatttctcc agcgcagtac ggcagatttt gtccgccacg tcgccaatac
    10501 gctccagctc ggcaatggtt ttactgatca ccataaccag tcgcaggtcg ctcgccgtcg
    10561 gctgacgttt ggcgataatg cgcacgcagg cttcatcgat cgccacttcc atcatgttga
    10621 cgttcttgtc gccttcgatc acgcgcttcg ccagatcgct gtcctggtta tgcatcgcgg
    10681 tgattgcatc agaaagctgc tgctccacca tgccgcccat ggtcatcacc tgcgtgcgga
    10741 tactttccag ttcggcgttg aactggccgg aaatatgttt attaagattg agactgtcca
    10801 taacgcactc ctgaatcaac cgtaacgacc ggtgatgtag tcttctgttt gtttcttcgc
    10861 tggcttggtg aacagatcgt ccgtgttgct gaactcaatc aattcgccca ggtacataaa
    10921 cgccgtgtgg tcggaacaac gcgcagcctg ctgcatgttg tgggtgacga tcaccacggt
    10981 gtaatcctgc ttcagttcgg tgatcagctc ttcaatacgc ccggtagaga tagggtcgag
    11041 cgccgaacac ggttcgtcga gtagcagcac ttccgggcga atggcgatac cacgcgcaat
    11101 acacagacgc tgttgctgac caccagagag agagtaaccg ctctggtgca atttatcttt
    11161 ggtttcgttc cacaatgcgg ctttggtcaa tgcccactgc acgcgctcgt ccatgtcggc
    11221 acgggagagc ttctcaaaca gacgaacgcc aaaagcgatg ttgtcgtaga tggacatcgg
    11281 aaacggcgtc ggtttctgga acaccatgcc cactttcgca cgcagcaggg cgatatcctg
    11341 agagttggtc aggatgttgt cgccatcaag cagaatttca ccttctgcgc gctgctccgg
    11401 atacagttca aacattttgt tgaaggtacg cagcagcgtc gatttaccgc agccggacgg
    11461 cccgataaac gccgttacct ggtttttagc gatatccagg ttgatgtttt tcagggcatg
    11521 gaatttgccg tagtagaagt tcaaattacg aacctgaatt ttactcgggg cagtttcaac
    11581 catactcatt gcaatctttt cctcatcgta cctgatgctt ttcgctaatc aggcttaccg
    11641 taggccgggt aaggcgttca cgccgcatcc ggcaaagtgt taaccgtgtt tattcttcgc
    11701 aaaaacaacg cgcgccagaa tgttcagcag caggacgcac agggtaatga tcaatacccc
    11761 ggcccaggcc agttgttgcc attccgcaaa cgggctcatc gcaaacttaa agatcgtcac
    11821 cggcaggttg gcgatcggct gcatcatgtc cgtgctccag aactggttgg agagcgcggt
    11881 aaacagcagc ggcgcggttt caccggcaat acgggcaatc gccagcagga taccggtcat
    11941 aatcccggac accgacgctt tcagcgtaat cgcggagatc atcttccact tcggtgtacc
    12001 cagcgcataa gccgcttcac gcaggctgtc cggcaccagt ttcagcatgt tctcggtggt
    12061 gcggataacg atcggcacct gcaacagcgc cagcgcaatt acgcccgccc agccggagaa
    12121 gtgctccatc tgtgccacca cgatggtgta gacaaacaga ccaaccacaa tcgacggcgc
    12181 ggagagcaga atgtcgttaa tgaagcgaat cacttctgcc agccaggatt tacgaccata
    12241 ttccgccaga taaatccccg ccataatgcc cagcggcgta ccgaataccg tggcccacaa
    12301 aattaacagc ccgctacccg ccagggcgtt cgccagacca ccaccttccg tattgggcgg
    12361 cggcgtcatt tcagtgaaca gcgccagcga cataccgtcg ataccgcgag tgatggtgga
    12421 cattaaaatc cagatcagcc agaacaggcc gaaggccatc gtcgccatcg agagcgtcag
    12481 cgcaatacgg tttttgaggc ggcgacgcgc ctgcattttg cggcgagatt cagccagcgc
    12541 cgcagtggtt tgcatttcaa ccatagccat tagcgtgccc cctcattctt agccaggcgc
    12601 ataatcataa acttcgatgc ggcgaggacg atgaaggtaa tcacaaacag gatcaggccc
    12661 agttccatca gtgcggcaac gtgcagaccg gattccgctt ccgcaaattc gttcgccagc
    12721 gcagaggtga tactgttgcc cggcatatac agcgaggcgc tgtcgagctg gtaggtgtta
    12781 ccgatgataa aggtcaccgc catggtttca ccgagcgcgc ggcccagccc cagcatgatg
    12841 ccgccgataa caccattttt ggtgaacgga agaacgatac gccagataac ttcccaggtg
    12901 gtgcagccaa taccgtaggc cgactctttc atcatcaccg gggtttgttc gaacacatca
    12961 cgcattaccg ccgcaatgta cggaataatc atgatggcga ggatcacgcc tgccgcgagg
    13021 ataccgatac caaatgcggg gccagagaac agcgcgccaa caatcgggat attcgacatg
    13081 atattgccga ccggctcctg aaagtaaacg gcgaacagcg gcgcaaagat aaacaggccc
    13141 cacatgccgt aaacgatact tggaatggct gccagcagct caatggcgat acccagcggg
    13201 cgtttcagcc agccaggcgc aagctcagtc aggaacaggg cgataccgaa actcaccggg
    13261 acggcgatca gcagcgcgat aaacgaagtc accaacgtac cgtagatcgg caccagcgcc
    13321 ccgtagatat cgttcggtgc atcccactct ttggtccata ggaaagccag accaaatttc
    13381 tgaatgctcg gccaggagga gatgatcaga gagacaataa tgccacccaa catcaatagc
    13441 acaatcagcg ccgccagttt taccagcacg ctgaaaatta tgtcgccctt tttacccggt
    13501 gggttaaaag caggcttggt tgcagccata agttactctt cagttaaacg cgtttacaaa
    13561 gtcattttgt aatgccggat gcggcgtaaa acaccgtacc cggcctggag ttttattagt
    13621 acagcggctt accgctactg tctttaatat tggtcttcca cgcagcgcga acctgttcaa
    13681 ctacactatc cggcaggctg gcgtaatcca ggtcgttcgc ctgtttagcc ccggttttgt
    13741 acgcccagtc gaagaatttc agcacttctg tgccttgttc tggtttcttc tgatctttgt
    13801 ggatcagaat gaacgtggta gaggtaatag gccatgcatc ttcgcctttc tggttggtca
    13861 gatcctgagc gaaggttttg ctccagtctg caccttttgc tgcattagcg aagttttctt
    13921 cggtcggact aaccggttta ccatcagcgg agatcagttt ggtgtacgcc aggttgttct
    13981 gcttcgcgta agcatattca acataaccaa ttgcacccgg cagacgctga acgaacgcgg
    14041 cgataccgtc gttaccttta ccgcccagac cgatcggcca ttttacggta gagccagtac
    14101 caacgttgtt tttccactct tcgttcactt tcgccaggta gctggtgaag acgaaggaag
    14161 tcccggagcc atctgcgcgg cgtactacag caatgttttg tgaaggcagt ttcagacccg
    14221 gattcagttt ggcgatggct tcatcatccc acttcttgat tttgcccagg tagatgtcgc
    14281 cgagggtttt accatccagc accagttcgc cagacttcag ccctggaatg ttaaccgcca
    14341 gcaccacgcc gccaatcacg gtcgggaact ggaacagacc ttcctgagcc agtttttcgt
    14401 cagacagcgg cgcgtcagag gcaccaaaat caacggtatt agcgataatc tgttttacgc
    14461 caccggaaga accgataccc tggtagttaa ctttattacc ggtttctttc tggtaagtgt
    14521 cagcccattt ggcatacacc ggcgcaggga aggttgcacc tgcacctgtc aggcttgctt
    14581 ctgcaaacac agagaaagcg ctcatcgata aggtcgcggc gacaacagtt gcgacggtgg
    14641 tacgcataac tttcataatg tctcctggga ggattcataa agcattgttt gttggctacg
    14701 agaagcaaaa taggacaaac aggtgacagt tatatgtaag gaatatgaca tttttatgac
    14761 agaaaggaat taccaaataa cttactgatt ttaaatgtga agatgaaaat cccacatgtc
    14821 atctttttgt catgatgcat ttaaaagtga taaagaaatg ggtgagatga caacgatgtg
    14881 atgaacaatc gttgtcagac ctattagcgc acaatctcaa cagttatcgt cgctgttgca
    14941 gtaaattgcc ccggggctgg gcgagctcca gtaaggctcg caggagcagc tgaaaaagtt
    15001 acactacctg atttttcacc atacactgtt gatttaccca tgtctgttgg caactcacca
    15061 ccgttcactt tgatttcctt attatttttg tcgtaaattt ttatccccac gttagagttt
    15121 gaagttacga ccattgaatt gtccgtattg ggttcggtag cataaaaaga cgtactgata
    15181 tcatcggtat tcagtatccc taggttttca cactcaatag tgaccgtttt agtaatttta
    15241 tgtgttgtta ccgcacttcc aacaacagat gaaaaatctg cagctggtat ttctttaaaa
    15301 tcaaaataaa ctggctgtaa attatttatt tcgcaacttt gcggagctgt aatatcgccc
    15361 cgaatataaa catcagccat cggctgtgag taatccaccg gggtgtcacg gctaattgtt
    15421 ccatacaatg tcactatctt tgtttcagga ataatcaatt ttcctgcaaa tgtcttcttg
    15481 atataaaaat atactatcgc tttagcccca ctagatatac ttaaaggttc aatgcggttg
    15541 caattgtact ttgtattacc agacgggttg ttagcatgat attcaaaagg tgcattcaca
    15601 tacccaaccc caagaatatc tatagcaatc ccaacatcaa ggttcggatt aagatcataa
    15661 taattaatat tagagtgaac cccagcataa gttaatgcct ggtttatttt tgacgtataa
    15721 tagatatagg ggaattctcc tacgttacca gtatcacaat gacagcgtcc aggatatgag
    15781 gatccattag ttacagtggt gttaaatgtc gttccaacct tattttcgtt cgcggtaata
    15841 agatatcccg agaaatcaag cgtactgtga aatacgccat tatcaggggt acagatacct
    15901 ttaggagctg caagtgctac actacttaac gagagcataa acacagcaaa gcaccactgt
    15961 ttgatatact tgttcataaa aatcctttta atgacatgtc acaccctgtt caattaatcc
    16021 gctctcactt tctggtgtcg aaagtgaata attgaagatg cattttgttg aggcgttttc
    16081 tccccaggat aacgtaacct gtcctttttc tggtagacca gaaagataaa gttcgccacg
    16141 gtcaccgaca attcctgaat ctgccaaact tgcatcttgt atcgcagcaa tggctccaaa
    16201 aggtaccggt ttaccatttg catttaacac tctgaagaga acgcgataac ccacatgagt
    16261 cacaaactcc gctttaacca ctgcaccacg cgtgggaacc atcgtaacga cggtattatc
    16321 aagttctata ttcgacttcg agaaatagtt atcgctgatt gctacccgat tttcatcata
    16381 agggtttagc tgtgtcttga cggtatagcc gcgccagtcc gtcgcagcac cgcgcatatt
    16441 atctatttct aacccggcgg ccccaggtgc tttaacaaga gcaatagttt cacctaactc
    16501 ctgacttaac gttacccctt cgctgtgtgc cagaattccc cctctgatac cataattcag
    16561 cctctgggaa ttttggctgt agttatagct gccgttcacc gtgccataag agccatcata
    16621 ggtcagggac atattaccgc caacctcatc atcatgactg ttataggact gcattaaatt
    16681 ccatgaaagg ttatttcttt ccagcgccgt accgctgagt ccgagttgat gtgagctacc
    16741 ggaattatcg ttttgcgaaa cagaataggt ggcgtaacta ttaccccacc agcccgataa
    16801 aggaacagat aaagagagca tgtagatctg ctcactggtg ttatcactgg catggttgtt
    16861 ccccgtcccc tgtctggcag tattggaata actccaggag acgttccacg agagacgctt
    16921 ccagctactg ctgtagccaa attgcattaa tcgttcgaca tcatcggtat gccagtattg
    16981 ttgccggctt aatgtcaggt ataaattgcc ataatctctc cccaacgact gcgttagcgt
    17041 tccctccacg cgactacgtc ggttacctgt ttcccaaaag tcttcagggc tgttgcgccg
    17101 cgatgcccac tcattgaggg tatagtagcc ctcggtggag taacgatagc caaccaggcg
    17161 caagctagtg tctgtgtcgt caaatcgttt tgaatagaga aagcgatatg actgacccgt
    17221 ttctgtatca tcatggctaa aattcgcacg agcatgtgtc acatcgaaag aaattgcgcc
    17281 caaagcgcca agatctttac cgacacccag agctgcagaa ccataatttt cagcagcctg
    17341 catacctcca tataaagtga tcccgtggga aaaaccatga agtatttgcg cctgaaggac
    17401 gtccggtgta aatccgtctt catcgcgcaa ttcacccacg ctgacatcaa catctgtctg
    17461 accttcacgt ttaagaatcg ccaatgaagc ataaggttgg gtgaatcgtt gttcgctacc
    17521 gtcttcttct ttgatggtta cgtccaaatc gccacctgta ctggcggtgt tgagatcggt
    17581 gatctcaaaa gcaccttgtg gaacggcaga ctgataaatc acgtagccat tctgccgaat
    17641 gatgacggtg gcgttggact ttgcaatccc acgaaccaca ggagcaaagc cattctggct
    17701 ggcgggtagc atatcgttat cagtatacaa acgcgcgccg cgaatttgcg tactgtcgaa
    17761 aatatcgctc gccgtccacg tatcaccaat cataatctgg cttcttaacg cggcaatatc
    17821 gcgctgtatc caggtactaa tggagttgta ttccgcacgg ccatcgctgg tgtttaaagt
    17881 actgtaatta cgtagccgcc acggtccaat attcagtcca ttgcgcagat tgagataact
    17941 ggtatctgtc tcttgtgaac cataatctga acgtagtgta tgtgaaccag aaaaatcgta
    18001 gttgatggtg agcgcgttaa tcccctcatc ccaacgcgtg gggctgatgt aatcataagc
    18061 catcgcgtcc atgtagattt ggggaatgga tatattcagc tgcaatttat cactgtcaaa
    18121 ctgccaggat gagccttcaa tacgtggagc aggatcgagg caaaccgtct catccacatc
    18181 attgaacgat ttgagcgcat caacttttat gccaaaggag gacagtgttt ttttaatggc
    18241 aataaagcaa gcgtgcagat cagcatctgg attatcctga acaaatgtca catcctgacg
    18301 ttcaaacatt ttaccgttga taaaaatatc aacgctataa acacctgggg gcatcaggtt
    18361 tgcccgatta aactggcgaa tatcgactcc ggattgatgg tcgatttcca gaagcgccgg
    18421 attgaataca ctgacctcgg ccatcaggtt ttgcgagaaa atcagacacg tccctgccgt
    18481 gaggccaacc actattctgg tcgtcatcat aatacgccta tccttatccc ttgcttacag
    18541 cgtcattttt atcgggtcac tgactgcgcc gtaatcatta ataaatcgcc aggtcatttg
    18601 gcctcccgca gaattagcag gtaaaggtaa cgtgagcgtt tcaaaaggac ggatcatatt
    18661 gaggggcaca tcatggccat caacgatgac atccatcaca ctgatatgaa ttgggttggg
    18721 attctctgct atcagatttc gcccttcccg gcgccatttc actttcggcg cagcatcttc
    18781 gggaataagg cggatatttg ctggtcgcca gaacagcttg atacgcgttt ttactgctat
    18841 ttccagcgag ttcgtcgctt tgttgctcgc tgatggcgga atatttttaa tattcagcca
    18901 gaagactgac tcgcgatcat caggcaatga cacacctttg gtgtgaataa tgcgtaatat
    18961 tttttcttgc gaaggttcta atcgtgatac tggcggaata acggtaaacg gcgctttgct
    19021 tttatcctct ttattaaatg gatctaccca agcctggatg agataaggta ttttttcttc
    19081 tttgctaaat acagaaatag atttatcagc attattactt aagtaaacca cccgcgtacc
    19141 accaatgaca acgcctgcat aagttaatgc tggttggcct aaaaaaacgc tgagtataat
    19201 cgcggtaaat aacttacatg aaactgttgt tttaaagagc ctgattaaac aactttttct
    19261 ggacataacg actccctggc caaagccaac tgaataaaaa ggcccttgat aaattatttc
    19321 ttatattcga cagtaaattg tgaatcagcg ttcgccgtac cagccgttac ggtcgcattg
    19381 gttgccacat agcgtgcaat aaacttcatt tcagccttgc cttcagcatc gatagcggta
    19441 taagcagagt cgttataaag tttaacctgc gaattatcgg actggttaaa aatacgaata
    19501 ccaacgccgg ttgccgcaat cgcttgtcct gtaccggtaa aatcaccagg gttgcctgct
    19561 gtaagcggcg tacccacttt caggtcgccg tcgccgccag gcgcttctgt accatcaaaa
    19621 cgaacggctg cttgagtgta cgttgccggg caattttcaa gtttaatact gaacttctgt
    19681 ggcgacgcca gactacctac acctgaaaat gcatttttac tgactttacc cagtgggaca
    19741 atttgatcgg ctgtatcgga ggtaacttca caagtagtgt cgacaatttc accgacgaag
    19801 tgaacaacac catcttctgc cagtgcatgc ccggccagca ttagagatgc cagagtgaat
    19861 gcaccgccta taatattacg cttcataaaa actccattga tataatatat cctttaccat
    19921 gaatcatcac ttatattaat aagtgtattt ttgattcaaa ggaatagtac accaaagaga
    19981 cggctttatc taaaaataat attaattacg caaaaataaa atataaattt attgtttaaa
    20041 tattatttaa acagtaaaaa atcaagtttg ttatcagaca ttaatgataa ttcagaatat
    20101 atttatatag aaagatagat gaatttattg atgtcagtta taaaaaaggg ccaccggtcg
    20161 gcagcccttt tttatctatg atagtgatta ctcaaccgta accgattttg ccaggttacg
    20221 cggctggtca acgtcggtgc ctttgatcag cgcgacatgg taagccagca gctgcagcgg
    20281 aacggtgtag aagatcggtg caatcacctc ttccacatgc ggcatctcga tgatgtgcat
    20341 gttatcgcta cttacaaaac ccgcatcctg atcggcgaag acatacaact gaccgccacg
    20401 cgcgcgaact tcttcaatgt tggatttcag tttttccaac agttcgttgt tcggtgcaac
    20461 gacgataacc ggcatatccg cgtcaatcag cgccagcggg ccgtgtttca gctcgcctgc
    20521 agcgtaggct tcagcgtgaa tgtaagagat ctctttcagc ttcaatgcgc cttccagcgc
    20581 gattgggtac tgatcgccac ggcccaggaa cagcgcgtga tgtttgtcag agaaatcttc
    20641 tgccagcgct tcaatgcgtt tgtcctgaga cagcatctgc tcaatacggc tcggcagcgc
    20701 ctgcagacca tgcacaatgt catgttcaat ggaggcatcc agacctttca ggcgagacag
    20761 cttcgccacc agcatcaaca gcacagttaa ctgagtggtg aatgctttag tggatgccac
    20821 gccgatttcc gtacccgcgt tggtcatcag cgccagatcg gattcgcgca ccagagaaga
    20881 acccggaacg ttacagattg ccagcgaacc aaggtaaccc agctctttcg acagacgcag
    20941 gcccgccagg gtatccgcgg tttcgccaga ctgtgacaag gtgatcatca ggctgttacg
    21001 acgcacggca gatttgcgat agcggaattc agaggcgatt tcgacgtcgc acggaatacc
    21061 tgccagcgat tcaaaccagt aacgggaaac cataccggag ttataagaag taccacaggc
    21121 gaggatctga atatgctcaa ccttcgacag cagttcgtcg gcgttcggtc ccagctcgct
    21181 taaatcaacc tgaccgtggc tgatgcgccc ggtaagggtg tttttgatcg cgttcggctg
    21241 ttcgtagatc tctttctgca tgtagtgacg gtaaatacct ttatcgcccg cgtcatattg
    21301 cagattggat tcgatatcct gacgttttac ttccgcgcca gttttatcga agatgtttac
    21361 cgaacggcga gtaatttccg caatatcgcc ctcttcaagg aagataaagc gacgggtcac
    21421 cggcaacagc gccagctggt cagaagcgat aaagttttcg cccataccca ggccaatcac
    21481 cagcggacta ccagaacgtg ccgccagcag ggtatccggg tgacgggagt ccatgatcac
    21541 tgtaccgtac gcaccacgca gttgcgggat agcacgcaga acagcctcac gcagagtacc
    21601 gccctgtttc agctcccagt tcaccagatg ggcaattact tcggtgtctg tttcagaaac
    21661 gaaggtatag ccacgcgctt ttaactcttc gcgcagcggt tcatggtttt cgatgatgcc
    21721 gttatgcacc accacaatgt gttcagaaac atgcggatgc gcattcactt ctgaaggttc
    21781 accgtgggtc gcccagcgag tatgagcaat acccgtaccg ccatgcagag gatgttcttc
    21841 cgctgcctga gccagcatct ggactttacc gaggcgacgc aggcgggtca tatgaccttc
    21901 cgcatcaaca acggccagac cggcagagtc atatccgcgg tattccagac gacgtaaacc
    21961 ttcaagaagg atttctgcta catcacgttg cgcgatcgcg ccaacaattc cacacatagt
    22021 ttttgattcc gatttatatc gttgtcggtc aacctgtatg cccgtttttt cgggcgcccc
    22081 gagccttgta gagagtgggg ttatttttat agttactgct tgtgggaggg agattatgtt
    22141 atctcctcat cccagatgac cggattaccc ggccagaatt actttttctt taccggacga
    22201 cgccagcctt ctttctgagt ctgcggcaca cggctgatag ctaatgcatt ttcgccgaca
    22261 ttacgcgtca cagttgtacc cgcagcaatg gtcgcgcctt tgcctactgt taccggggcc
    22321 accagctgag tgtcggaacc gacaaacaca tcgtcgccga taatggtctt aaatttattc
    22381 gcaccatcgt agttgcaggt aatggttccc gcgccgatgt taacgttatc gccaatttcc
    22441 gcatcgccca ggtaagtcag atgaccagct ttcgagcctt tacccagacg cgcttttttc
    22501 atctcaacaa agttaccgac gtgtgcacct tccagcaact cagcaccagg acgcagacgg
    22561 gcaaacgggc caatagtaca ggccgctgcc agattcgcgt cttccacaac ggtatacgga
    22621 ctgatttcgc aatcatcgcc aatcacgctg tttttaatca cgcaaccggt gccgattttc
    22681 acgcgatgac cgagagtcac gttgccctcg atgataacgt tagtatcaat ttcaacatcg
    22741 cgcccgtgag taagcgtacc gcgcagatca aaacgcgccg gatcgcgcag cataacgcct
    22801 gctaacagca gtttttcagc ctgttcggac tggtaaacgc gctccagacg ggagagttgc
    22861 aggcggttat tcacaccttc tacttcgctt aaacgttgcg gatgaacggc gacgatttca
    22921 cgcccttcct gatacgccag cgcaataatg tcggtgatgt agtattcgcc ctgagcatta
    22981 ttgttggtca gcttcgccag ccagcgtttc atatctgcgc cgttagcgat cagaatgccg
    23041 gtgttgatct cctgaatctg acgctgctcg tcggtggcat ctttgtgctc aacaatgccg
    23101 gtaactttgc cattttcacg ggtgatacgt ccataaccgg tcggatcatc cagtttcacc
    23161 gtcagcagac caatgccacc ctgcggttta gcatcacgca ggcgctggag tgtttcgaca
    23221 gagatcagcg gcacgtcgcc gtagagcatt aaaatgtctt catcatcggc aaagaaaggt
    23281 gcggcctgct gcattgcatg acccgtaccc agctgctctg cctgaagcac ccagttaagg
    23341 ttgtcgtctt tcagcgcctg tttaagcaga tcgccgccgt gaccgtacac caggtgaacg
    23401 tgcgctgcgc ctaattcatt cgcagcatca atgacatgct gaaccatcgc tttcccggca
    23461 agggtatgca gcactttcgg aagatcggaa tacatgcgcg tgcctttgcc tgcggcaagg
    23521 atcactacgc tcatagcatt attcaacata cgcgtcctga ctgtaatttg agaacgaatt
    23581 taaaccgctt caccttgaaa aaactacatt tttttcatcg tgaaatggac agaggataaa
    23641 ttgttcaata acggattatc cccgctgacg aaagtgccat tttcgaccat cgtcacgcct
    23701 tttgtctgca gaaaattaag gcagtacctt catttaagcg tcaggtggat gtttttgctc
    23761 ttatttcgat caatgaataa acgggaaaac aggggtgttt tatatcagca ggatctatgt
    23821 gaacgctctt caggacggat cacgcgtaaa aaaaagccag cctgtttcca gactggcttt
    23881 tgtgcttttc aagccggtgt tacatcgctt ttttggtcaa ctcgataacg cgcagctgcg
    23941 cgatcgcttt ggccagttcc gcagacgcct gagcgtaatc tacgtcgccg tgagagctgc
    24001 taatgtgctc ttcagcctta cgtttcgctt ccatggctcg cgcttcgtcg agatcctggc
    24061 cgcgaattgc ggtgtcggcc agaacggtca cgttgccagg ctgcacttca agaatgccgc
    24121 cagacagata gataaactct tcgtgaccgt gctgtttcac gatgcgaatc ataccaggct
    24181 taatggcggt gagcagcggt gcgtggccag ggtagatccc cagttcacct tcgctacccg
    24241 ttacctggat tttctcgacc agaccagaga acatttgttg ctctgcgctg acgacgtcca
    24301 ggtggtaagt cattgccata tcaccctccg attaaggcgt taaagttttt tggctttttc
    24361 cacagcttct tcgatggaac cgaccatgta gaacgcctgc tccggcaggt gatcgtattc
    24421 gccttccatg atgcctttaa agccacggat ggtgtctttc agggagacgt atttacccgg
    24481 agaaccggtg aatacttctg ccacgaagaa cggctgggac aggaagcgct ggatcttacg
    24541 agcacgcgct accaccagtt tgtcttcttc agacagttca tccataccca ggatggcgat
    24601 gatgtctttc agttcctgat aacgttgcag gatggactga acgccacgcg cggtgtcgta
    24661 gtgttcctga ccaaccacca gcgggtccag ctgacggctg gtggagtcca gcgggtcaac
    24721 ggccgggtag atacccagag acgcgatctg acggctcagt accacggttg cgtcaaggtg
    24781 cgcaaaggtg gttgccggag acgggtcagt caagtcatcc gcaggtacgt ataccgcctg
    24841 tacggaggtg atagaaccag ttttggtgga ggtgatacgt tcctgcagaa cgcccatctc
    24901 ttccgccagg gtcggctgat aacctaccgc tgaaggcata cggcccagca gtgcggatac
    24961 ttccgtaccg gccagggtgt aacgatagat gttgtcaacg aacagcagaa cgtcacgacc
    25021 ttcgtcacgg aatttctcag ccatggtcag accggtcaga gcaacgcgca gacggtttcc
    25081 cggcggctcg ttcatctggc catacaccag ggatactttg tcgataacgt tggagtcggt
    25141 catttcgtgg tagaagtcgt taccctcacg agtacgttca cctacgcccg caaacacaga
    25201 gtaaccggag tgctcgatcg cgatgttacg aatgagctcc atcatgttta cggttttacc
    25261 tacacccgca ccaccgaaca gaccaacttt accgccctta gcgaacggac acatcaggtc
    25321 gataactttg ataccggttt ccagcagttc ctgagagttt gacagctctt cgtaggaagg
    25381 tgctgcgcgg tgaatcgccc aacgctcttc ttcaccgatc tcgcctttca tgtcgaccgg
    25441 ttcacccagt acgttcatga tacggcccag agtcgcttta cctaccggga cttcgatcgg
    25501 gtgttcgagg tcttttacat ccagaccgcg acgcagaccg tcggaggaac ccattgcgat
    25561 ggtacgcacg ataccgccgc cgagctgctg ctgaacttcc agcaccagac gctcattacc
    25621 attttgcacc tcaagagcat cgtacacgcg cggtacggca tcctgaggga attcgacgtc
    25681 aactacggcg ccgattacct ggacaatctt tccagtagcc atcttaaatc ctctacgaaa
    25741 taacctgttt aaaccgcggc ggcccccgag acgatctcgg tgagttcctg agtaatgctg
    25801 gcctgacgag ctttgttgta taccaactgc agctctttaa tcaggctgcc gccattgtcg
    25861 gtcgcggctt tcatcgccac catacgggcg gcctgctcgc tggccaggtt ttcaaccacg
    25921 ccctgataaa cctgagattc gacataacga cgcagcaggg tatccagcaa cgccttcgga
    25981 tcgggttcgt acaggtaatc ccaggattta tgtttcagat catcatcatc tgatgccggt
    26041 aacggcagca gctggctgat ggtcggaacc tgagacatgg tgttaataaa tttgttgctg
    26101 acaatgtaaa gcttgtccag acggccttcg tcgtaggcct gcaacatcac ttttaccgga
    26161 ccgatcagtt cggacaggga agggttatcc cccatgccgg tgacctgggc aacaacattg
    26221 ccgcccacgg agttgaagaa cgacacgcct ttcgagccga tcattgcgag gtcgcattga
    26281 acgcctttgt cggtccaggt cttcatttcc gccagcagtt ttttgaacag gttaatgttc
    26341 aaaccaccgc acaaaccacg gtcggtcgac accaccaggt agcccacgcg tttaacgtcg
    26401 cggtcttcca ggtaagggtg cttatattcc agattaccgt gtgcaaggtg accaatcact
    26461 ttgcgcatgg tttctgcata aggacggctg gccgccatgc gatcctgcga tttacgcatt
    26521 ttggaagcgg cgaccatctc catcgcttta gtgatctttt gcgtgttctg gacgcttgcg
    26581 atcttactac gtatctcttt tgcgccggcc atgagcttct cctcaatgcc ttgcggcctg
    26641 ccctaaggca agccgccaga cgttaccagg attgggttgc tttgaaggaa tcgaggatgc
    26701 ctttcagctt gccttcgatt tcgtcgttgt agccaccggt ctggttgatc tcttgcatca
    26761 acggagcgtg atcacggtcg acgtaagcca gcagagcggc ttcgaagctg ccaattttcg
    26821 acagttcaac atccgccagg taaccacgtt ctgctgcgaa cagaaccaga gactgctgcg
    26881 caacggacat cggcgcatac tgtttctgtt tcagcagttc ggtcactttc tgaccgtggt
    26941 caagctgctt acgtgttgca tcgtcaaggt cggatgcaaa ctgagagaac gctgccagtt
    27001 cacgatactg tgccagagcg gtacggatac caccggacag ttttttcatg atcttggtct
    27061 gtgctgcacc accaacacgg gatacggaaa tacccgggtt aaccgcagga cgaataccgg
    27121 cgttgaacag gttggtttcc aggaagatct gaccatcggt aatggagatt acgttggtcg
    27181 gaacgaacgc agaaacgtca cccgcctgag tttcgataat cggcagtgcg gtcagagaac
    27241 cggttttccc tttcacttca cctttggtga aggcttcaac gtattcggcg ttaacacgcg
    27301 cagcacgctc cagcagacga gagtggaggt agaatacgtc gcccgggaat gcttcacgtc
    27361 ctggcggacg acggagcagc agggagatct gacggtaagc aacagcctgt ttagacaggt
    27421 catcgtaaat gatcagcgca tcttcaccgc ggtcacggaa gtattcgccc attgcgcaac
    27481 cggcatacgg agccagatat tgcagtgcag cggattcaga cgcggttgct accacaacga
    27541 tggtgttagc cagtgcgccg tgctcttcca gtttacgtac cacgttagaa atggtggacg
    27601 ctttctggcc gatagcgaca tagatacatt tgataccgga atcgcgctgg ttgatgatgg
    27661 catcgatagc cagtgcggtt ttaccggtct gacggtcacc gatgatcaat tcacgctgac
    27721 cacgaccaat tgggatcatg gagtcaacgg ctttataacc ggtctgtacc ggctgatcta
    27781 cggactgacg ttcgataacg cccggagcga ttgcttctac agcagagaag ccgtcgtgat
    27841 ccagcggacc tttaccgtcg attggtgcac ccagagtgtt aaccacacgg cccagcaggc
    27901 cacggccaac cggaacttcc aggatacggc cagtacactt aactttcatg ccttcggcaa
    27961 ggtcagcgta cggacccata acaaccgcac ctacagagtc gcgctcgagg ttcagtgcga
    28021 tagcgtaacg gttacccggc agggagatca tttcaccctg catacaatcg gccaggccgt
    28081 gaatgcggat aacaccgtca cttacagaaa caatagtacc ttcgttgtga gcttcactca
    28141 caacattgaa ctgagcaatg cgctgcttga tcagttcgct gatttcggtg gaattcagtt
    28201 gcatgctcca gtccccttaa gactgcaaga cgtctgcaag gcgctcaaga cgaccgcgta
    28261 cgctgccatc aatgaccata tcacccgctc ggatgataac gcctgccatt acagacttat
    28321 cgattttgca attcagctta actttgcgtg acagacgttt ttccatcgca gcagaaattt
    28381 tcgcgagctg ttgttcactc agtgcggcag cggaaatgac gtctacctca gcggtagcct
    28441 cactcacggc acgaaggtga ataaactgct ccagaacatc cgggagcgcg ttaagacgac
    28501 cattttcagc cattacccga atcaggttct gaccgttttc gtccagttgc tcaccacaaa
    28561 ctgcgataaa cgactcggcg agcgtttctg gcgcaagcgc gccagagaga agctctgcca
    28621 tttgttcgtt tttggttacc tcggcggcaa acgccagcat gtcctgccag cgttctacac
    28681 tttggtgttc gacggcaaag tcaaaagctg ctttggcgta ggggcgagct accgtaataa
    28741 attcagacat cagcccctcc ctccttacag ttcagcgaca agtttatcca cgatgtcgct
    28801 gttagcagct tcatccacgg aacgttcgat gatcttctcg gcgccagcaa cagccaggat
    28861 agcaacttgc ttacgcagct cttcacgggc acgtttacgc tcggcttcaa tttctgcctg
    28921 cgcctgggcc acgattttag tacgttcctg ttctgcctca gctttcgctt cgtccagaat
    28981 ctgcgagcgg cgtttgttcg cctgctcgat gattacctgg gcttccgctt tcgctttttt
    29041 cagctggtcg gtcgcgctgg cctttgcaag gtcaaggtcc ttatgtgctc gttctgcgga
    29101 agcaaggccg tcagcaattt ctttttgacg tttttcgatg gctgccatta atggcggcca
    29161 tacgtacttc atgcagaaca gaacgaacag gacaaacgcg atggcctggc cgaggattgt
    29221 tgcgttaaga ttcacagcac aatgcctcta tttagttaac gttctgatat tgctctttaa
    29281 ataaaagcaa cgcttactac gcgacagcga acatcacgta cagacccaga cctacagcga
    29341 tcatcgggat agcatccacc agacccataa cgataaagaa ctgagtacgc agcagaggaa
    29401 tcagatcagg ttgacgcgct gcgccttcca ggaatttacc cccgaggatg ccgataccga
    29461 tcgcagcacc gattgccgcc agacccatca tcacagcggc agccatgtac agcagatcca
    29521 tattcaggtt ttccatgaca gtctccagtt tgtttcagtt aaaacgtagt agtgttggta
    29581 aattaatgtt cttcagacgc catcgacaga tagacgatcg tcagaaccat gaagatgaag
    29641 gcttgcagcg taatgatcag gatgtggaaa atggcccacg gcacattcag gatccactgt
    29701 gaccaccacg gcaacagacc agcaatcaga atgaaaatca gctcaccggc atacatgtta
    29761 ccgaacagtc gcaaaccaag tgaaactggt ttggacagca ggcttacccc ttcaaggatt
    29821 aagttgacag gaatgaacgc ccagtgattg aacggctgca gcgtcaactc tttcgtgaag
    29881 ccgccgatgc ctttcatttt gatgctgtag aacagaatca ggataaatac gcccaatgcc
    29941 atagacagcg ttacgttcac gtccgcagac ggaaccacac gcagtgcagg cagacccagt
    30001 acatgttcag caatgtacgg cagcaggtcg ataggcagta aatccatcag gttcatcagg
    30061 aatacccaga cgaagatcgt cagggccagc ggagcaatca gcttgctttt gccatggtac
    30121 atgtctttca cgctaccatt aacaaagccg atcaccagct caatcgcggt ctgaaactta
    30181 cctggcacac cgctggtcgc ctttttggct acgctacgga ataaaaccag gaacaacaga
    30241 cccagcacca ccgagaagaa catggagtca atattgattg tccagaaggt ggctgggggg
    30301 ttttgtggat ccaccagcga gaatgtacgc aggtccagct gaaggttatt caggtggtgt
    30361 cctatgtaat cctgcggcgt catattttct gaagccatga tgccttttac cctttgttgt
    30421 taattacagc cggtgccagt atctgaacca ccagcaccaa aacccacgta acgatcagcg
    30481 gcaagaatac cgcctgtaaa accgccaacg ccaccaccag taacaccaac atcgccagaa
    30541 ctttgaaagc ttcgccaaat gcgaatgtcc aggccacccg gcctttcgct ggtgtatgcg
    30601 cctggtgacg ccaggcaaat atcataaaca aaacgttagg cagaaagact gccaggcccc
    30661 cgcttattgc agagacgccc cagaaggggt ctttgaggct gaacagcaat ccacttgcta
    30721 tcaccaccag taactgaacg agcagaagct tccgagcaac gtttcgactc acgagcgaca
    30781 cagacatcac gtttttcact cctgctccct tcgaggtatg ccgcgtgtcg tataaaactt
    30841 tctttaaggc ttagagtcaa gcatcaaaaa gcggtcaaat tatacggtgc gcccccgtga
    30901 tttcaaacaa taagtagcca aaaggtgaat aaatgtttaa atatttttcc agtgcataca
    30961 attgcgactt ttctgctaac cctgttcgat catgaaaaac tgtaaataac gcgtaaacac
    31021 tggtgataaa gcgtgcttca gatcacatat tgcgcatgtt cgcgcacagc atatttattt
    31081 acttggcaaa tgatgccttt gcaagtttat gatatttcag tctaaaaaca gatactgttt
    31141 taataaatga catttacaca acaaaaacca cccattgaca tttttaataa tgttttaaca
    31201 gccaatgatg gttcttagcg ccgattttta gcagactgat attttcacta atgacttatt
    31261 ttctgcttac caaaaaaagc cacgttatct tgttgatgca aaagagtgaa cgtggcgtta
    31321 aatgtaacca gttatatcag tagaaaacct ggttgttgtt aacagtctaa ccggtcaatt
    31381 ttttatgatt tttttgataa aaattaaatt ttatttgctt taatcaccac cagatgacgt
    31441 tcgccatcca gggctggaac ctgaagttta accactgatt cgacctgata ttcttcgggc
    31501 aacaaagcga tttcatcttc cggcatttgc cctttcagcg cgtagaaacg gccttgctca
    31561 ccaggaagat ggtggcacca gctcaccata tcgttcagag aggcaaaagc gcggctaatt
    31621 acgccatcaa atggcggctc tgaaggaaac tcttctaccc tgctctgtac tggttcaata
    31681 ttctccagtt taagctcatg ttgcacctga cgaaggaaac gcacgcgttt accaaggcta
    31741 tccaacagag tgaaatgggc ttcaggacgc acgatagaga gtggaatgcc tggcagtcca
    31801 ggtccggtgc cgacatcgat aaaccgttca ccttgcagat acggtgccac cacaatgcta
    31861 tcgagaatat ggcgtaccag catctcatta ggatcgcgga ccgaagtcag gttgtacgct
    31921 ttgttccatt tatgcagcat attcacgtag gcaataagct ggtttttctg gtgatcggta
    31981 agcgaaatac ctgcgtcttt cagcagtaag gagagtttgt tgagcacggt gattacctgt
    32041 tcttgatgcg ttgcctggta agcgggtgct taccaggcat ttttaatgcg ttatgcgcta
    32101 cgacgcagca taccctgttt tttcagccac accagcagaa tggagatggc cgcaggcgtg
    32161 acgccagaaa tacgcgaagc ctggccgata gaggctggtt tgtgatcgtt aagtttggcg
    32221 atcacttcgt tagaaagacc ggatacctgg cggtaatcca gtgtcgcggg tagcagggtg
    32281 ttctcgttac gcagctgctt ttcgatctca tcttgctggc gcgcgatata accttcgtat
    32341 ttaacctgaa tctcaacctg ttccgccgcc tgttcgtctg tcaacgcagg ggcaaacggc
    32401 gtcagcgtgg ttaatttttc ataagtcatt tccggacgac gcagcagatc ttcaccactg
    32461 gcttcacggg aaagcggcgc agtcaggtga gcattcactt cggctgcagc ttccgccgac
    32521 ggggttaccc aggtcgattt cagacgctga cgctcacgct cgatattctc aagtttctcg
    32581 ttaaagcgcg cccaacgttc gtcatccacc aggcccagtt cacgaccgat ttcagtcaaa
    32641 cgcagatccg cattatcttc gcgcagcatc agacgatatt ctgcgcgcga ggtaaacata
    32701 cgatacggtt ctttggttcc taaagtgcac aggtcatcaa ccagtacgcc gagatacgcc
    32761 tgagaacgcg ccggagccca accttctttg tcagcagaca gacgggcagc gttaagaccg
    32821 gccagtaaac cttgcgcagc ggcttcttcg taaccggtag tgccgttaat ctgaccagca
    32881 aagaacagcc cctggataaa cttgctctcc agcgtcggtt tcaggtcgcg aggatcgaag
    32941 aagtcatact caatggcata acccggacgc acgatcttcg cgttttccat cccctgcata
    33001 gagcggacga tttgcatctg cacatcgaac ggcaggctgg tggagatacc gttcggataa
    33061 atttcgttag aggtcagtcc ttccggttca aggaagatct gatgctgatt tctgtcggcg
    33121 aagcgcataa ctttgtcttc gatcgacggg cagtagcgtg ggccgacacc ttcgatcacc
    33181 cctgcataca ttgggctacg atcgaggtta ctgcggatca catcatgggt tttctcgttg
    33241 gtatgagtga tataacacgg cacctgctgg ggatgctggg acgcattgcc cataaacgag
    33301 aataccggca ttgggttatc gccatgctgt tgcgccagca cactaaagtc gatagttcga
    33361 gcatcaatac gcggtggtgt cccggttttc agacgaccaa cgcgcagcgg cagttcacgc
    33421 aaacggcgag aaagcggaat ggacggcgga tcaccagcac ggccaccgct gtaattatcc
    33481 agaccgatat gaattttacc gtcgaggaac gtcccaacgg tgagcacgac ggctttggca
    33541 cggaacttca gtcccatttg ggtaacagca ccgaccacgc gatcgttttc gacaataaga
    33601 tcttcaaccg cctgctggaa gatcatcagg ttcggttggt tctccagcgc cgtacgtacc
    33661 gcctgacggt agagcacacg atccgcctga gctcgggtag cgcgaaccgc cggtcctttg
    33721 cttgcgttta gtatcctaaa ctggataccc gcttgatcga tcgctttcgc catcagaccg
    33781 ccgagtgcat ccacttcttt taccagatgt cccttcccaa taccgccgat cgccgggttg
    33841 cagctcatct gccccagagt gtcgatattg tgtgtcaaaa gcagagtctg ttgacccata
    33901 cgcgccgcgg ccatcgcggc ctcggtgcct gcatgacccc cgccaatgat gatgacgtca
    33961 aaaggatccg gataaaacat ggtgattgcc tcgcataacg cggtatgaaa atggattgaa
    34021 gcccgggccg tggattctac tcaactttgt cggcttgaga aagacctggg atcttgggta
    34081 ttaaaaagaa gatctattta tttagagatc tgttctattg tgatctctta ttaggatcgc
    34141 actgccctgt ggataacaag gatccggctt ttaagatcaa caacctggaa aggatcatta
    34201 actgtgaatg atcggtgatc ctggaccgta taagctggga tcagaatgag gggttataca
    34261 caactcaaaa actgaacaac cgttgttcct tggataacta ccggttgatc caagcttcct
    34321 gacagagtta tccacagtag atcgcacgat ctgtatactt atttgagtaa attaatccac
    34381 gatcccagcc attcttctgc cggatcttcc ggaatgtcgt gatcaagaat gttgatcttc
    34441 agtgtttcgc ctgtctgttt tgcaccggaa tctttgagtt ctgcctcgag tttatcgata
    34501 gccccacaaa aggtgtcata ttcacgacta ccaataccga ttgcgccaaa gcggaccgca
    34561 gaaagatcgg gcttctgttc ctgcaatgct tcatagaaag gagaaaggtt gtccggaata
    34621 tctccggcac cgtgggtgga gctgataatc agccagatcc ccgaggcaga taaatcttcc
    34681 aacagcggac cgtgcagcgt ttcggtggta aaacccgcct cttccagctt ttcagccagg
    34741 tgttctgcta catattcggc accgccgagg gtgctgccgc tgataagagt gatatctgcc
    34801 ataaaccgcc acctttatta agagtggcgt attgtacgct gtgaacgcgt tgggatctac
    34861 ctgtggaaaa gtatgggatt aaaaaagccg atcagggctt gatagtacgc atgatcgggt
    34921 tttgcaggac gatcaatgtc tcggtggact gaatttcatc aattgtttgg atcttgttga
    34981 taagtacatg ctggagagcg tcgatcgaac ggcacattac ttttataaag atgctgtagt
    35041 ggcctgttgt gtaataggct tcagtgactt catcaaggct ttccagcttt gccagcgcgg
    35101 aagggtagtc tttggcgctc tttaatataa tgccgataaa gcagcctacg tcataaccga
    35161 gctgcttcgg gctgacatca atacgcgccc cggtaatgat ccccgcctgc ttcattttct
    35221 ctacccgaac gtgaatcgtc cccggactga cgccaaattg tttcgccagt tcggcgtaag
    35281 cggtgcgcgc attgcccatt aatgcttcca ggatgccacg gtccagattg tcgatcagat
    35341 aattttccat aggattttct tatgcagatt gatgattcat tctattttag ccttcttttt
    35401 taatgaatca aaagtgtgtt aggcttttta ttgaatgatt attgcatgtg tgtcggtttt
    35461 tgttgcttaa tcataagcaa caggacgcag gagtataaaa aatgaaaacc gcttacattg
    35521 ccaaacaacg ccaaattagc ttcgtgaaat ctcacttttc tcgccaactg gaagaacgtc
    35581 tggggctgat cgaagtccag gcgccgattc ttagccgtgt gggggatggc acgcaggata
    35641 acttgtcggg ctgtgaaaaa gcggtgcagg taaaagtgaa agttctgcct gatgcccagt
    35701 tcgaagtggt tcattcactg gcgaagtgga aacgtcagac cttagggcaa cacgacttca
    35761 gcgcgggcga agggctgtac acgcacatga aagcccttcg ccccgatgaa gaccgtcttt
    35821 ctccgttgca ctcggtctat gttgaccagt gggactggga acgcgtaatg ggcgacggtg
    35881 agcgtcaatt ctcgactctg aaaagcacgg tagaggcgat ctgggcggga attaaagcaa
    35941 ccgaagctgc ggttagcgaa gagtttggcc tggcaccgtt cctgccggat cagatccact
    36001 tcgtacacag ccaggagtta ctgtctcgtt atccggatct tgatgccaaa gggcgtgagc
    36061 gggcgatagc gaaagatctt ggcgcggtat tccttgtcgg gattggcggc aagctgagcg
    36121 atggtcatcg ccacgacgtg cgcgcaccgg attatgatga ctggagcacc ccgtcagagc
    36181 tgggtcatgc gggtctgaac ggcgatattc tggtgtggaa ccctgtactg gaagatgcgt
    36241 ttgagctttc ttccatgggg atccgcgtgg atgccgacac gttgaagcat cagttggcgc
    36301 tgaccggtga tgaagatcgt ctgcagctgg aatggcatca ggcgctgctg cgcggtgaaa
    36361 tgccgcagac catcggcggc ggtatcggcc agtctcgttt gactatgctg ctgctgcaac
    36421 tgccgcatat cggccaggtt cagtgtggag tatggccagc tgctgttcgc gagagcgtcc
    36481 cttctctgct gtaataattt atcgccgcca gcgtctgagc aggcggcttc gcatcccggt
    36541 atcaaagcgc cagatatgat cgaaaatgcg catgatgccg ggtttgccgt gtgccgacat
    36601 cgccacggca tgaaagcgat gctgatgtac ccgctgcagc tctttcactt tactcgtcac
    36661 gtcgtcaggc aaccgctgag cgataaaatc agaaatcacc accgcatcgg catcaaacca
    36721 ttccctgctt tgcaagcgtt ccataatggc gcgaaaacaa ctggcaagat cggtgccgcc
    36781 acgaaactgc tggcttaaaa aacggattgc ttgttcgatg ccttgtgggc ctgaaagctc
    36841 ataacggacg atctcggtgg aaaatagcat aatatagcag cgccggtttt ctgcgagagc
    36901 aatgcgcatc aaggccaggc agaacgcttt cgcacactgt tcattaaagc cgcccattga
    36961 gccggaagta tccacacaga caataaacgg cccgcgcggc tgttcatcgt aatctttatg
    37021 taccaccgga cgttcgatca ctttttcacg ccacgactca ccgtgcaggc gataggtgag
    37081 caactgtttt tccaccagcc gacggtaaaa ctcatactcc agttccgtta tccctagtgt
    37141 cgccagttct ggcggcagga gacgtaaaat atcatcgctt tgttgcagac catcaacctg
    37201 ctcaggaacc gtcgccggtt cgcgcaccat ggtgcggaag gtttccatct gcgcatcgtt
    37261 gcgcggtatt gatttggctt cccgagaacg ccccagctgc tctgccaggc gtttcagttc
    37321 cggctgttcg ttaagaaatt caccgtattt cacaatcaac tgatagtcgc cacgtttaag
    37381 ctgaccggcg ctcatatccc acagacgacc agctgcagta ttgttatctg cgagaatcgg
    37441 ttcaagttgt ccgctcagcg tcatgcgttc ctgaacttca ctcaacagtt gttcgcgttc
    37501 ttcttctaat agctgttgat ttaacgtcgt tgcttgcacg atcagactta aacgccaacg
    37561 ctggagaaaa agcgtgtgta acgctgaagt gatcgtgctg ttagcatcaa ccaactgtcg
    37621 ggcttgttct gcccatggag aattcagacg atgcagtaag tccaggatct gtggtagctg
    37681 cacaataaac tgtggcgtgg agaggagctg gctttgctga tagcacatca cctcttcggt
    37741 gagttccggc gggactcggg catctttcag ccgactgcgc agcgcttcac gccagcgggg
    37801 aacatcatca gtgattgccg ccttcaggcg tgggaatttt tcaaagaaga ccgccagctg
    37861 cggtgaggcc agcagtgcga tgatcatctc ttcgatcaat ccctcttcgc tgacggccag
    37921 catcacatta agcgtatcca gcgttagcat tgttgtgcct ggcgaatctg tgcaccgaca
    37981 tcctgtaggc tggcttcaat gcgacctaac cagtcgccag ggataaacag acatttttgc
    38041 tgttcgctga ataaagcgtg ttgcttgcgc cagtcgcttt caagctcttc cagttgttgt
    38101 tttatttcac caggcagacc ttcagccgat gaaccgggaa gtgccagcgt actgccttgt
    38161 aaacttacat cgcgaacgac aagatgttgg gcgctatcaa cttccagatt cagtttctgg
    38221 gcaaagccga taccgttcag tttaccgcga atttcaccac ctttgctcag ccactgttcc
    38281 agcgcgctac gctcaaagga gatatgaacc acttccatat catgcagttt taacggtttt
    38341 tgcagcagta gagtcagagt ggaagcagta acgttaacag gaagttgata ctgctgacga
    38401 cggctgaaaa tgccgcccag acgaattacc gttaaggctg ttttatcgct ttgttgctgc
    38461 tggagttgca ggtgacgttg cacaatcgcg ccaaggcgag tcaacatccc ttgctgttgc
    38521 caggcgtgac cggtcatcaa tacatcaatt tgttgttgta tcaaattcag gctttgcgcg
    38581 tcataccaca ggcaatcttt cagcaaaatg agatctaccg gggcaacagc actgcgaccg
    38641 ctaaaaaagg cgctggcctg caataatcgg atcgcttttt tccagcgacg atccgagaca
    38701 taaggcgcat ccggtaattt atccagttgc tggcgcagca taaaaatcag ctcaaataca
    38761 tgatcgggca gcgtaatttc accaatctct ttctgccagc gttcatattc ttcatctgtg
    38821 acctgcaagg catcaggaac cggattgtcg ttttcatcct gttgactggt cagcatggag
    38881 cggaaattcg ctttatcctg cactttatct aaccacagac gaatcagcat gcggtcatat
    38941 aacgcttcca gactgctgtc tgcttccggc agctcgttgg aggccgccac cagcagacgc
    39001 atcgggattt tttctacgtg tgcaccgttg cggaactggc gctcgttaat ggcggtgagc
    39061 aaggtattaa gaattgccgg gcccgctttc cagatctcat ccagaaagac aatttctgct
    39121 tccggcaggt aaccgctggt taaacgttca tagcgccctt catcttttag cgcctgaata
    39181 gaaaggggac caa
//
LOCUS       DAMZET010000022        39048 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.022, whole genome shotgun sequence.
ACCESSION   DAMZET010000022 DAMZET010000000
VERSION     DAMZET010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.022"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..581
                     /locus_tag="POB34_001275"
     CDS             <1..581
                     /locus_tag="POB34_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HDI5918260.1"
                     /translation="EVGENLITASHALLPGWTIPHWQVPDEVKLPKTLTLVYHLPIEL
                     HTMAERLQATLAAEGCELTIIFHNAKNWDDTTLLAHADLMMGDRLIGEAPEYTLEQWL
                     RCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEENRFNALKAVFSQLMADATLTPQFNYH
                     YRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ"
     gene            646..1341
                     /gene="queC"
                     /locus_tag="POB34_001276"
     CDS             646..1341
                     /gene="queC"
                     /locus_tag="POB34_001276"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDI5918261.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1393..1791)
                     /gene="fadM"
                     /locus_tag="POB34_001277"
     CDS             complement(1393..1791)
                     /gene="fadM"
                     /locus_tag="POB34_001277"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HDI5918262.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(1885..2256)
                     /locus_tag="POB34_001278"
     CDS             complement(1885..2256)
                     /locus_tag="POB34_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDI5918263.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAAPAAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(2407..4278)
                     /gene="ppiD"
                     /locus_tag="POB34_001279"
     CDS             complement(2407..4278)
                     /gene="ppiD"
                     /locus_tag="POB34_001279"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDI5918264.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQTAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(4470..4742)
                     /gene="hupB"
                     /locus_tag="POB34_001280"
     CDS             complement(4470..4742)
                     /gene="hupB"
                     /locus_tag="POB34_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HDI5918265.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(4951..7305)
                     /gene="lon"
                     /locus_tag="POB34_001281"
     CDS             complement(4951..7305)
                     /gene="lon"
                     /locus_tag="POB34_001281"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDI5918266.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(7493..8767)
                     /gene="clpX"
                     /locus_tag="POB34_001282"
     CDS             complement(7493..8767)
                     /gene="clpX"
                     /locus_tag="POB34_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HDI5918267.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(8893..9516)
                     /gene="clpP"
                     /locus_tag="POB34_001283"
     CDS             complement(8893..9516)
                     /gene="clpP"
                     /locus_tag="POB34_001283"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDI5918268.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(9762..11060)
                     /gene="tig"
                     /locus_tag="POB34_001284"
     CDS             complement(9762..11060)
                     /gene="tig"
                     /locus_tag="POB34_001284"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDI5918269.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(11404..11721)
                     /gene="bolA"
                     /locus_tag="POB34_001285"
     CDS             complement(11404..11721)
                     /gene="bolA"
                     /locus_tag="POB34_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HDI5918270.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            12026..12604
                     /locus_tag="POB34_001286"
     CDS             12026..12604
                     /locus_tag="POB34_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDI5918271.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            12648..14123
                     /gene="ampG"
                     /locus_tag="POB34_001287"
     CDS             12648..14123
                     /gene="ampG"
                     /locus_tag="POB34_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HDI5918272.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            14583..15530
                     /gene="cyoA"
                     /locus_tag="POB34_001288"
     CDS             14583..15530
                     /gene="cyoA"
                     /locus_tag="POB34_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HDI5918273.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            15552..17543
                     /gene="cyoB"
                     /locus_tag="POB34_001289"
     CDS             15552..17543
                     /gene="cyoB"
                     /locus_tag="POB34_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HDI5918274.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            17533..18147
                     /locus_tag="POB34_001290"
     CDS             17533..18147
                     /locus_tag="POB34_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HDI5918275.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            18147..18476
                     /locus_tag="POB34_001291"
     CDS             18147..18476
                     /locus_tag="POB34_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HDI5918276.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            18488..19378
                     /gene="cyoE"
                     /locus_tag="POB34_001292"
     CDS             18488..19378
                     /gene="cyoE"
                     /locus_tag="POB34_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HDI5918277.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            19527..20891
                     /locus_tag="POB34_001293"
     CDS             19527..20891
                     /locus_tag="POB34_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5918278.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(21019..21510)
                     /gene="yajQ"
                     /locus_tag="POB34_001294"
     CDS             complement(21019..21510)
                     /gene="yajQ"
                     /locus_tag="POB34_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HDI5918279.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            21678..22589
                     /gene="panE"
                     /locus_tag="POB34_001295"
     CDS             21678..22589
                     /gene="panE"
                     /locus_tag="POB34_001295"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDI5918280.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVATPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            22552..23142
                     /gene="yajL"
                     /locus_tag="POB34_001296"
     CDS             22552..23142
                     /gene="yajL"
                     /locus_tag="POB34_001296"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HDI5918281.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(23196..24644)
                     /gene="thiI"
                     /locus_tag="POB34_001297"
     CDS             complement(23196..24644)
                     /gene="thiI"
                     /locus_tag="POB34_001297"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDI5918282.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            24850..25092
                     /gene="xseB"
                     /locus_tag="POB34_001298"
     CDS             24850..25092
                     /gene="xseB"
                     /locus_tag="POB34_001298"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDI5918283.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            25092..25991
                     /gene="ispA"
                     /locus_tag="POB34_001299"
     CDS             25092..25991
                     /gene="ispA"
                     /locus_tag="POB34_001299"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HDI5918284.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            26016..27878
                     /gene="dxs"
                     /locus_tag="POB34_001300"
     CDS             26016..27878
                     /gene="dxs"
                     /locus_tag="POB34_001300"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDI5918285.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            28058..29032
                     /gene="yajO"
                     /locus_tag="POB34_001301"
     CDS             28058..29032
                     /gene="yajO"
                     /locus_tag="POB34_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HDI5918286.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSFSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGRNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(29086..29604)
                     /gene="pgpA"
                     /locus_tag="POB34_001302"
     CDS             complement(29086..29604)
                     /gene="pgpA"
                     /locus_tag="POB34_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDI5918287.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(29582..30559)
                     /gene="thiL"
                     /locus_tag="POB34_001303"
     CDS             complement(29582..30559)
                     /gene="thiL"
                     /locus_tag="POB34_001303"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HDI5918288.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(30637..31056)
                     /gene="nusB"
                     /locus_tag="POB34_001304"
     CDS             complement(30637..31056)
                     /gene="nusB"
                     /locus_tag="POB34_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDI5918289.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(31076..31546)
                     /gene="ribE"
                     /locus_tag="POB34_001305"
     CDS             complement(31076..31546)
                     /gene="ribE"
                     /locus_tag="POB34_001305"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDI5918290.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(31635..32738)
                     /gene="ribD"
                     /locus_tag="POB34_001306"
     CDS             complement(31635..32738)
                     /gene="ribD"
                     /locus_tag="POB34_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDI5918291.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(32742..33191)
                     /gene="nrdR"
                     /locus_tag="POB34_001307"
     CDS             complement(32742..33191)
                     /gene="nrdR"
                     /locus_tag="POB34_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HDI5918292.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            33342..33881
                     /locus_tag="POB34_001308"
     CDS             33342..33881
                     /locus_tag="POB34_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDI5918293.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            34180..35064
                     /gene="tsx"
                     /locus_tag="POB34_001309"
     CDS             34180..35064
                     /gene="tsx"
                     /locus_tag="POB34_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HDI5918294.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(35241..35588)
                     /locus_tag="POB34_001310"
     CDS             complement(35241..35588)
                     /locus_tag="POB34_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HDI5918295.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(35717..36688)
                     /gene="secF"
                     /locus_tag="POB34_001311"
     CDS             complement(35717..36688)
                     /gene="secF"
                     /locus_tag="POB34_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HDI5918296.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(36699..38546)
                     /gene="secD"
                     /locus_tag="POB34_001312"
     CDS             complement(36699..38546)
                     /gene="secD"
                     /locus_tag="POB34_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HDI5918297.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(38574..38906)
                     /gene="yajC"
                     /locus_tag="POB34_001313"
     CDS             complement(38574..38906)
                     /gene="yajC"
                     /locus_tag="POB34_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDI5918298.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(38929..>39048)
                     /gene="tgt"
                     /locus_tag="POB34_001314"
     CDS             complement(38929..>39048)
                     /gene="tgt"
                     /locus_tag="POB34_001314"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Exchanges the guanine residue with
                     7-aminomethyl-7-deazaguanine in tRNAs with GU(N)
                     anticodons (tRNA-Asp, -Asn, -His and -Tyr); Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDI5918299.1"
                     /translation="YYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
BASE COUNT         9081 a        10361 c         9773 g         9833 t
ORIGIN      
        1 tagaagtcgg agaaaacctg atcaccgcca gtcatgcatt actgccaggc tggactattc
       61 cgcactggca agtaccggat gaagtcaaac taccgaaaac cttgacgctg gtttatcact
      121 taccgataga acttcatacc atggcagaac gcctacaggc gacactggca gcggaaggct
      181 gtgaactcac aattattttt cataacgcaa aaaactggga cgacacgacc ctactggcac
      241 acgcagacct catgatgggc gacagattaa ttggcgaagc accggaatat actctggagc
      301 aatggctacg ttgcgatcca ctatggccac atgttttcga cgctccagca tatgcacatc
      361 tgcaatcgac gctggacgcg gtgcaagtaa tgcctgatga ggaaaaccga tttaatgccc
      421 tgaaagcggt ttttagccag ttaatggcag acgcgacgct gacgccgcag ttcaactatc
      481 actatcgcat tagtgcccct cccggcgtga acggtgtgcg actgacaccg cgcggctggt
      541 ttgaatttac cgaagcctgg cttcccgcgc cgtcgcaatg aatagctggt ccgggttaat
      601 cgcaggcgtt accataacgc ttttattgtt caagcaggat tatctatgaa acgtgctgtc
      661 gttgtgttca gtggaggcca ggattccacc acctgtctgg tgcaggcatt acaacaatat
      721 gatgaagtcc attgcgtgac gttcgattac ggtcagcgac atcgcgcaga aatcgacgtg
      781 gcacgcgaac tggcgctgaa actgggagca cgcgcgcata aggttcttga tgtaacgctg
      841 ctcaacgagc tggcggtcag cagcctgaca cgtgacagca ttccggtgcc tgattatgaa
      901 cctgaagccg atggcatccc gaatacgttt gtcccagggc gtaatatttt gttcctgacg
      961 ctggcggcaa tatatgcgta tcaggtaaaa gcagaagccg taattaccgg cgtctgtgaa
     1021 acggatttct ccggctaccc ggattgccgc gatgagtttg tgaaagcact aaaccatgcc
     1081 gtcagtttgg gcatggcgaa agatattcgt tttgaaacgc cgctgatgtg gattgataaa
     1141 gcggaaacct gggcgctggc agattattac ggcaaactgg atttagtccg taacgaaacg
     1201 ttgacctgct ataacggcat taaaggcgac ggttgcggtc attgtgcggc atgtaattta
     1261 cgcgccaacg gtttgaatca ttatctggcc gataaaccga cggtgatggc agcgatgaag
     1321 cagaaaaccg ggttgaggta attattccgg gtgtcgccgg atgcggcttg agcatccggc
     1381 accacaaaac gtttacttaa ccatctgctc cagcttttcg cgcaattccc cttccagagc
     1441 taatgctttc tgcgttttaa gatcaataca aacaaacgta ataagcgcat ccgctaccac
     1501 ctgcccttcc ggctccagtg taatgacctg gcttaagatg ccgcttttac cgtttaattg
     1561 ctgcaactga ctggtaatag ttaacaggtc acttaatacc gctgggcgac gatagttaat
     1621 attgatattg accacgacga aggcgatgtt atgggccgtc atccactgaa aactgtcgct
     1681 attttccaac ccatcccagc gggcttcttc gagaaattca aggtagcggg cgttgttgac
     1741 gtgctggtaa acgtcgagat gatatccacg aactttgatt tgtgtttgca tagcgcaata
     1801 accttacgtt gttgttataa gtacagaggt cataactggt atgacctctt cagtctggca
     1861 aaaaattgcc actatgcaaa ttaattacag ggttaatacc gccagattac gttccaccag
     1921 cgaattgccc atccccggca cctgctttag atcctccacc gttttaaacg gaccgtactc
     1981 ttcgcgataa ctgacaatcg cctgcgcttt cttcaggcca acgccattca tcgcgcgggc
     2041 tagctcttcc gcgctggcat tattaatgct gacccgggtg ccttcttcgt cactggcttt
     2101 cgccggtact gctgctttac tttgtgctgc aggagcttcc gctttggttt ctaccgttgt
     2161 cggtttcgcc gcaggagctg ccgccagcgc gctatgagac attccggcac aagccagaga
     2221 cagggtaatg agcagtgctt taattccgtg tttcatactg ttttctcctt gtttgttaac
     2281 agtggaatca ccttaacggc agtgaaaaca gtaaacaaat ggcaatgttc agaaatggaa
     2341 aaggccgcga aagcgacctt ttcttgttac agctgcaacg catttttttg cgaggcggct
     2401 tcaggattat tgctgttcca gcgcatcgcc aattttgatt ttcgcctctt tacgcaggtt
     2461 actcatcaga gcttcaaaga cgatttgtgc gttgttctgg gtgataccct gcaccatcgc
     2521 ttttttctga tcttccggca ttgaaccttg tttcacttca tccagcgcca gcagaaccac
     2581 attaccttgc atatcggtcg ccataccgta gctcggtttg tctttcgctg gcagtggcag
     2641 tgcaaacgcc gcctggctaa tcgggtcacg accggaacgg cttaaggttt tcggctcgcc
     2701 aaatttcaga ccggcagcct gcatagcttc cgcacctttg ccggctttca aatcaaccag
     2761 cagtttctca gcatccactt tcgcctgttg ttcagctttg ttgtgctgaa ccaatgcctt
     2821 aacttgttcc tgaacatctg ccaacggttt caccgcttcc ggtttgtgct cgctgatgcg
     2881 cagcacgaat gcgcggtcgc cgtctacggt gatgatgtca gagttgatgc ccggcgcgcc
     2941 gttttcacct accagaccgc cgttaaagat agcgtcggca accggcttga agttcaactc
     3001 ttccggcagg ttatctttgc tgaaccaacc cgtctgagtg gctttaacgc cggcagtttg
     3061 ctctgcaccg gccagagact cggtgtcgtt gcttgccgca tcgctcactt tctgctgcag
     3121 cgcatagtac gcatcgaggg ctttttcgtg tttcactttc gccgcaatgt cgtcacgtac
     3181 ttcgtctaac gatttcactt tcgctggctg aatgtcgtcc agacgtacaa tcaggaaacc
     3241 gaccgaagat ttgatgacac cagacagttg ccctttttct ttcagaccag catttttcag
     3301 ttcgtcgggg atagtggcat cttctaacca acccatatcg ccgccgttac gggcagagat
     3361 aatatcggca gatttttctt tggctaatgc agcaaaatca ccgcctttat tcagctcatc
     3421 aagtaccgct ttcgcttcat cttcagtttt ggtctggatg atgctgtagc gggtacgctg
     3481 cggctgggtg aattgatcct gatgctgatc gtagtagctc tggatatccg catcgctaac
     3541 cggttgctgc atcgttgcgg catccagctt gatgtaactc acgcggaatt gttccggcgt
     3601 catgaaattg tttttgtttt gttcgtagta gctggcaatc tcctgttcgg tcacaggctg
     3661 cttcgccgcc agcgcgttaa catcgatagt cgcctcacgc accacgcgtt gttgcgcgac
     3721 cagtgccgcc agctcgtcgg tttcaccttt cagcataaaa tcggtaccgg caacaccgtt
     3781 aatcagctgt tgggtagtga gctggttacg cagcgcctgg gcgtactgat cggcggtcat
     3841 ccccatctgg ttgaggatac cgttatagcg gctgttatca aatttgccgt caacctggaa
     3901 ggctggggtc gcgaaaatcg cctgtttaac ctgctcatca ctgataccca gtttcagctc
     3961 acgagcgtac tgatccaaca gcgcctcgtc gatcagacga ttcagcacct gttgacgcag
     4021 ggttttcata tagccttcgt tcgctgccag ctcggagtat tgatcgccca gctgttgctg
     4081 catgcgatta cgctcgctgt taaaggcgtt ctcgaactgc ccacggctga tttcctggtc
     4141 attcactttt gcggcgtagt tattgcctcc gccaatcagg taaccactca cgccggtcaa
     4201 tatgaacgac acgataatga taccgaaaat aatcttgagc acgagactgt ttgcagccgt
     4261 gcgtaagctg tccatcatgg tgtaacaaca ctccgctgta gatgactgtg tacctcacgc
     4321 aacgtatcga tgcgcgcaaa ggcctattgt gacaagaaac gggggcaatt gtcagcccac
     4381 aagacgcaga aagtcaccga atacaaataa aaaaggcaca tcagtagatg cgcccttgaa
     4441 cttcgtcaca tccccaccgg ggacaacgct tagtttaccg cgtctttcag tgctttacct
     4501 gcacggaagc tcggtacttt agcagcagcg atggtgatct ctttaccggt ctgcgggttg
     4561 cggccagtac gggcagcacg ctctttaacg gcaaaagtac caaaacctac cagtgctaca
     4621 tcatcccctt ctttcagaga ttcagttacg gaagcaataa tagcatctaa cgcacggcca
     4681 gccgcagctt tagagatatc agcccctgca gcaatcttgt cgatcaattg agatttattc
     4741 actcttctct tcctctttat aatttatatc gcacttgaat ccttcaaggt acgaacgcgc
     4801 agcagttata tcaggccagc catcccctta caacacccct taataagggc aagcccgatc
     4861 cgccatctaa cttagcgaga caaaaaaagg ctggcaagcc cgaattagcc tgccagccct
     4921 gtttttatta gtgcattttg cgcgaggtca ctattttgca gtcacaacct gcatgccaga
     4981 cggttcattt tgcagcgcca gagtcagaac ttcctcaatg cgcttcacag gatgaatgtc
     5041 cagatcggca attacgttgt caggaatctc ttccagatcg cgtttatttt cgaacggaat
     5101 tagcactgtt ttaatcccgc cgcgatgcgc tgccaggagt ttttctttca aaccaccgat
     5161 cggcagtacc tgaccacgca gagtgatctc accggtcatt gccacatcgg cacgaaccgg
     5221 gttaccggtc aggcaagaaa ccagcgcggt gcacatagca ataccggcac tcggaccatc
     5281 tttcggcgtc gcaccttccg gtacgtggac gtggatgtca cgtttttcgt aaaaatcagg
     5341 gttgatcccc agtttttccg cacgcgcacg aaccaccgtt aacgccgcct gaatagactc
     5401 ctgcatcact tcgccgagcg aaccggtata ggtcagtttg cctttacccg gaacacatgc
     5461 ggtttcaatg gtcagcaagt caccgcccac ttccgtccac gccagaccgg ttacctgacc
     5521 gacacggttt tcgttatcag cgcgaccata gtcgaaacgc tgaacaccga ggtagtcatg
     5581 caggttatcg ccgttaattt cgatatgttt taatgactta tcgagcagta actgcttaac
     5641 cgctttgcgg cacagtttgg agatttcacg ctccagacca cgcacgcccg cctcacgggt
     5701 gtagtaacga ataatgccga taatggcgct atcgtcgacg gtcagctcac cttttttcag
     5761 tgcattacgt tcaatctgct tcggcagcag gtgacgtttg gcgatgttca gtttttcatc
     5821 ttcggtataa ccggagaggc gaatcacttc catacgatcc agcagcggtg ccggaatgtt
     5881 catggagttc gacgtcgcga caaacatcac gtcgctgaga tcgtaatcca cttccaggta
     5941 gtggtcgctg aacgctacgt tctgctctgg atccagcact tcaagcagtg cggaggccgg
     6001 atcgccacgc atgtcagaag acattttgtc gatctcatcg agcaggaaca gcgggttttt
     6061 cacgcccact ttcgccattt tctggatcag tttacccggc atagaaccga tgtaagtacg
     6121 gcggtgacca cggatttccg cttcatcacg cacgccgccc agcgccatac ggacatattt
     6181 acgcccggtg gctttggcga tggactgacc aagagaggtt ttacctaccc ccggcggccc
     6241 taccaggcag aggatcggtc ccttgatttt gttgacacgg ctttgaaccg caaggtactc
     6301 aaggatgcga tctttcacgc gctccagacc ataatggtcg gtatcaagga tttcctgcgc
     6361 ctgacgcagg tcttttttga ccttgctgcg cgcattccac ggtacctgta ccatccagtc
     6421 gatataacca cgcactacgg tcgcttctgc cgacatcgga gacatcattt tcagcttctg
     6481 caactctgct tccgctttct cttttgcttc tttcggcatc ttcgccgcgt cgattttgcg
     6541 cttcagggct tcgttttcgt ccggcgcgtc gtccatttca ccgagttctt tctgaatagc
     6601 tttcatttgc tcgttcagat agtactcacg ctgggatttc tccatctgct ttttaacgcg
     6661 gttgcgaatg cgtttctcaa cctgcagcag atcgatttcc gattccatca ttgccatcag
     6721 atattccaga cgttcgttaa cgtcggacat ctccagaacg gactgtttgt cagccagttt
     6781 cagcggcata tgtgcagcaa tggtatccgc cagacgcgcc ggatcgtcga tgctattcaa
     6841 cgacgtcagc acttctggtg ggattttttt gttcagcttg atgtagcctt cgaactggct
     6901 gattgcagta cgcaccagca cttcctgttc tcgctcatca atggtcggcg actccagata
     6961 ctccgccttc gcagaaaagt gttcgccatt gtcagagagc gcagaaatac gcgcgcgctg
     7021 taacccctcg accagcactt tgacggtgcc gtcaggcagc ttcagcatct gcaatataga
     7081 ggccacggtc ccgacggtga aaagatcgtt tacacccggc tcatccgttg aagcttcttt
     7141 ctgcgcgacc agcataattt ttttatcatg gtccatcgcc gcttccagac aacggataga
     7201 tttttcccgc ccgacaaata aggggatgac catgtgcgga taaaccacca catcgcgcag
     7261 cggcaatacg gggatttcaa tgcgttcaga acgctcagga ttcatagagc tctctcttag
     7321 tttaatttcc gccaggtaat cagatgacac gactgtgctt cacgccatct attaacatgt
     7381 acgtcagtat atggggatgt ttcccccaca ttcaacgccg agaatagagg aaaaattaaa
     7441 ggggagataa aatcccccct ttttggttaa ctaattgtat gggaatggtt aattattcac
     7501 cagatgcctg ttgcgcttcc ggcttgccat aaatcagcaa cggtttgctt tgaccatcaa
     7561 ttaccgactc gtcgataacc actttttcga cgtcttccat ggacggcaga tcgtacatgg
     7621 tatcgagcag tgcggcttct acgatggaac gcaggccacg ggcaccggtt ttacgcgcca
     7681 tcgctttctt agcgatagca tccagcgcct cgtcacggaa ttccagatcc acgccttcca
     7741 gattaaacag cgcctgatac tgcttggtca gggcgttttt cggctctttg aggatctgaa
     7801 tcagagcttc ttcgctcagt tcattcaacg ttgcgacaac cggcagacga ccaatgaact
     7861 cagggatcag accaaacttg atcagatctt ccggttcaac ctgcgccagc agctcgcctt
     7921 cgcttgcttt gtcggacttc gcttttaccg tcgcgccaaa accaatgccg gagccggttt
     7981 ctacacgatg ggaaatcact ttatccagac cggcaaacgc accgccacaa ataaacagga
     8041 tcttagaggt atcaacctgc aagaattcct gctgcggatg tttacgccca ccttgcggtg
     8101 gaacagcagc taccgtacct tcgatcagtt tcaacagtgc ctgctgtacg ccttcaccgg
     8161 aaacgtctcg ggtaatggac gggttgtctg acttacgaga aatcttgtcg atttcatcga
     8221 tgtagacaat accacgctgt gctttctgga catcgtagtc gcatttctgc aacagcttct
     8281 gaatgatgtt ttcaacgtct tcacccacat aaccggcttc ggtcagtgta gtcgcgtcgg
     8341 ccatggtgaa cggaacatcc agcaggcgcg ccagcgtttc agccagcagc gttttaccgg
     8401 aaccggtcgg accgatcagc agaatgttac ttttgcccaa ctcgacgcca ttgctggtat
     8461 cgccgttgcg cagacgtttg taatggttgt ataccgcgac cgccagcact tttttcgcct
     8521 gttcctggcc gataacgtaa tcgtccaggt ggttgcgaat ttcatgcggc gtcggtagcg
     8581 cactgcgttc acgatgcggt gcaacttctt taatctcttc gcgaatgatg tcgttacata
     8641 aatcaacaca ttcgtcgcag atatacacgg atggaccggc aatcagcttg cgcacttcat
     8701 gctggctttt gccgcaaaaa gagcaataca gcaatttgcc tgagccatct ttgcgtttat
     8761 ctgtcatggg tcaaaacctc ttctttgttc tttgtgccgc acacgacgac gcaaatgcca
     8821 ttctcaggcg caagccgctt acagcgttgt gccgccctgg ataagtatag cggcacagtt
     8881 gcgcctctgg catcaattac gatgggtcag aatcgaatcg accagaccgt attccaccgc
     8941 ttcaggggcg gaaaggaagc gatcgcgctc ggtatcacgt tcaatctgtt ctaatgattg
     9001 acccgtatga agcgccataa gttcattcat gcgcccttta actttcagaa tttcacgggc
     9061 atgaatttcg atatcggtcg cctggccctg gtagccgccc aacggttggt gaatcatcac
     9121 gcgcgaattc ggcaggcaaa aacgtttacc ttttgcccct gcggtcagca agaaagcgcc
     9181 catcgaggcc gcctggccca tacagatggt gctgacatca ggcttgataa actgcatggt
     9241 gtcatagata gacatcccgg cagtgatcac cccgcctggg gagttaatgt acagatagat
     9301 atctttttct gggttttccg cttccaggaa cagcatctgc gccacaatca ggttagccat
     9361 gtggtcttca acctggccag tcagaaaaat gacgcgttcc ttaagtagac gagaatagat
     9421 atcaaaagag cgctcaccgc gtgaggtctg ttcaatgacc atcggcacca gcgccatatg
     9481 gggtgcaaag ttatctcgtt cgccgctgta tgacatttcc gtctcctgga taaaattgaa
     9541 aaaacctgct gtaccgattg taaccttatg gacggattat cagctagtcc ctgtgttatg
     9601 ggtacggcat caccatattt caagcataac aatcttttgt tgttacgcta acaccgaaag
     9661 aggcttttcg cacggttcca tgcaaaattc atgacaaaaa aaagcccacc acctggcggt
     9721 gacgggcctt tgtgcgaatt tagcgcgtta tgctgcgtaa attacgcctg ctggttcatc
     9781 agctcgttga aagtggtttc tttttcagtc actttcgctt tcgccagtac agcttcaaca
     9841 gcctgttctt ccagagcaac attgcgcatg ttgtccatca gttctttgtt tttgctgtag
     9901 aactcgataa cttctttcgg atcttcgtac gcagaagcca tctcttcgat caggcctttc
     9961 acgcgctctt cgtcagcttt cagctcgttg gtgcggataa cttcgcccag cagcaggcca
    10021 acaactacgc ggcgtttagc ctgttcttcg aacagttcgc gcggcagttc cagagcttgt
    10081 ttttcgttgc caccgaaacg ctgtgcagcc tggcgacgca gaacgtcgat ttcgctgtcg
    10141 atcagcgcag ccggtacgtc gatgtcgtta gcttttacca gaccttcgat cgcctgagac
    10201 ttaacgcggt tacggatggc gctcttcagc tcgcgctcca tgtttttacg cacttcagcg
    10261 cgcagacctt ctacggaacc atcttcaacg ccgaaacgtt tgatgaattc tgcagtcagt
    10321 tccggcagtt cacgctcttc aactttcttc aggttgatag cgaatttcgc tgctttacct
    10381 ttcaggtttt ctgcgtggta ttcttccggg aaggtcacgt cgatggtgaa ctcttcgcca
    10441 gctttgtggc ctttgatacc gtcttcaaag cccgggatca tacgaccctg gcccatcgcc
    10501 agtacgaaat cagacgcttt accgccttcg aactcttcgc cgtctacaga accggtgaag
    10561 tcgatggtta cgcggtcttc tgcttcaaca gcgccgtctt tttctttcca ggtcgcctgc
    10621 tgtttacgca gagtatccag catgccgtca acgtcagcgt cggtcacttc aacgatcggt
    10681 ttttcaactt cgatcgcttc cagaccctgc agttcaactt ccggataaac ttcaaactct
    10741 acagagtaag taaagtcttc acccagcttg tattcgcccg gaacataagt cggtgcgcca
    10801 gccggattga ttttttcttt aatgatggcg tcaatgaagt tacggctcat caggtcaccc
    10861 agaacgtcct ggcgtacaga cgcgccataa cgctgagcaa cgatattcat tggcactttg
    10921 cctttgcgga agccgtcaat acgtactttt ttcgcaacgt tgaccagctc gcttttaaca
    10981 gcggtctcga tgctgtcagc agcgatagta atcgttacac ggcggccaag gccttgagtg
    11041 gtttcaactg aaacttgcat cttgttacct caaaaaatca cagtgctcgg tcaactctta
    11101 ccccgcaagc acaggttgtt ggcttcgcgg aaccgggatg ttcttataat caatcacatt
    11161 ccctgttacc agaatcatcc cgaaagcatt caaataggac gcggcattat agcggcatca
    11221 cttttatgag tcgagaacgg ttattgcgcg ctgtcgcgta ttttttcaca attcccgggc
    11281 aattttcgtc tgaatgctgg gcaaaaagcg caaaatccag acaaaacaaa acggcccgca
    11341 ggccgttttt cataaaatct ttagcaacat actggaaaag cgccgacagt tgcaaatgcg
    11401 tttttacgcg atgcttcctg ctccacgaca gggaggagag gcaaagacgg tgtcctgcaa
    11461 cccttcccac tccttaatag tgtaagtatg cagagccagc gcatgaacgg tagtagagag
    11521 ttcctccgct aaagtactgt aaatcattcg atgacgattc agaaaacgtt cacccgtaaa
    11581 acgatcgctg accagcacaa ctttaaaatg gctttcagag ccggctggga cattgtgacg
    11641 atagctttca tccactactt cgaggaatac gggttggaac gccgccctta atttttcttc
    11701 tatccgctca cgtatcatca tgaaatatct cctccgacaa cgctgagatg tcatccatcc
    11761 ctttaaatac tagccgcttt taccgtttcc attgcagctt aacaacaaat atttaggttt
    11821 cgtcctggtt tttccgtcaa gcgtatgaag tttactgatt taaagattga aacacaggcc
    11881 tcgcatcagt gtttctttta ccaggggcga ttatttcgtc aacatgatga atttacatgc
    11941 cttacccact tcccctcgcc gttggcaatg ttatgatggc gggaattttt accaaacacg
    12001 cttaagagtc tctgagagcc cgaacatgtt caaaaaaatc ctcttcccgt tagttgctct
    12061 gtttatgctt gcaggatgcg caaaaccgcc aacaactatt gaagtttccc cgacgattac
    12121 actgccacag caggatccaa gcctgatggg cgtcaccgta agcattaatg gtgccgatca
    12181 gcgtaccgat caggcgctgg caaaagtcac ccgcgataat caaatcgtta ccctgaccgc
    12241 ctcccgcgat ctgcgtttcc tgctgcaaga agtgctggaa aaacagatga ccgcgcgtgg
    12301 ttacatggtt ggcccgaatg gcccggttaa tctgcaaatc atcgttagcc aactgtatgc
    12361 tgacgtgtcc cagggcaacg tgcgctacaa catcgcgacc aaagcggata tcgccatcat
    12421 cgctaccgcg cagaatggca acaaaatgac caaaaactat cgtgccagct acaacgttga
    12481 aggtgcgttc caggcctcca acaaaaatat cgccgatgcg gttaacagtg tgctgaccga
    12541 taccatcgct gatatgtctc aggacaccag catccacgaa ttcatcaagc agaacgcgcg
    12601 ttaatttcta ccctctggcc ctgtgcaagc cgggcctgta gacgcccatg tccagtcaat
    12661 atttacgtat ttttcaacag ccgcgttcag ccatattgct gatcctgggt tttgcttccg
    12721 ggctaccgct cgccctgaca tccggtacct tacaggcctg gatgacggtc gagaatattg
    12781 atctcaaaac cattggtttc ttctctctgg taggccaggc ttacgttttt aaattcctct
    12841 ggtcaccgct gatggaccgc tacacgcccc cattttttgg gcggcggcgc ggttggctgc
    12901 tcgccacgca aatcctgtta ttagtcgcca ttgcggcgat gggttttctc gaaccaggca
    12961 cccaactccg ctggatggcg gcgctggcgg tggtgatcgc tttttgctcg gcctctcagg
    13021 atattgtctt cgatgcgtgg aaaaccgatg tgctcccggc agaagagcgt ggtgcgggcg
    13081 cggcaatcag cgtgctgggt taccgtttag ggatgctggt ttccggcggc ctagccctgt
    13141 ggctggcaga taaatggctg ggctggcagg gcatgtactg gttgatggcg gcgctgttga
    13201 tcccctgtat tatcgcgacg ttgcttgcac cagaaccaac cgacaccatt cctgtgccca
    13261 aaacgctgga acaagcggtt gttgcacctc tgcgagattt ctttggtcgc aataatgcct
    13321 ggcttatttt gcttcttatc gtgctgtata agctgggcga cgcattcgcc atgagcctga
    13381 caaccacgtt tttgattcgc ggcgtcgggt ttgatgcggg tgaagtaggc gtggtaaaca
    13441 aaacgcttgg cttactggcg accattgtgg gcgcattgta cggtgggatt ttgatgcagc
    13501 gcctgtcgct gttccgggcg ctgctgattt tcggcatttt acaaggtgcg tctaacgctg
    13561 gttactggct gctgtcgatt actgataagc atctctacag catgggcgca gccgtctttt
    13621 tcgaaaacct ctgtggcggg atgggcacat cagcctttgt cgcgctgtta atgacgctgt
    13681 gtaataagtc attttccgct actcaatttg ccctgctctc agcgctttcc gctgtaggac
    13741 gagtttatgt cggccccgtg gcgggttggt ttgttgaagc acacggctgg tcgacattct
    13801 atctattctc cgtcgccgct gccgtaccgg ggcttatttt gctgctggtt tgccgccaga
    13861 cgcttgaata tacacgagta aatgacaact ttatctcccg taccgaatat ccggcaggtt
    13921 atgcctttgc catgtggaca ctggcggcgg gcgtcagcct gttggccgtg tggttactgc
    13981 tgttgacgat ggacgcgctg gatttgacgc acttctcttt cctgcctgct ctgctggaag
    14041 tcggggtttt agtcgccctt tctggcgtcg tgcttggtgg tttgctggat tatctggcgc
    14101 tacgaaaaac gcatctgacg taatctgtaa atattattta gctgtagtaa tcactcgccg
    14161 taattattac ggctaaataa acatcagtat cgcttaataa tatttgttct tttgtgcggc
    14221 ttagcgtttg gaaattattg acgccattta taaattctag ttttcttaca cgattcagct
    14281 aatgagtctt tattttctca tcacccagtt gtcactccaa tgataattat ttgttaaata
    14341 attgttttat ttcgcattgg ttataccaat tgcccgccca gactccttct gcactcccct
    14401 tttgttataa cgcccttttg caacagcttc ttaaaatcaa cctgatatgt tttgcaacat
    14461 atgtgacctg acagccaaat ccaagtaaca ggaatttaat catgtttaca gtaatgtaac
    14521 cttcccgtaa aatgcccaca cactttaaac gccaccagat cccgtggaat tgaggtcgtt
    14581 aaatgagact caggaaatac aataaaagtt tgggatggtt gtcattattt gcaggcactg
    14641 tattgctcag tggctgtaat tctgcgctgt tagatcccaa aggacagatt ggtctggagc
    14701 aacgttcact gatactgacg gcatttggcc tgatgttgat tgtcgttatt cccgcaatct
    14761 tgatggctgt tggtttcgcc tggaagtacc gtgcgagcaa taaagatgct aagtacagcc
    14821 cgaactggtc acactccaat aaagtggaag ctgtggtctg gacggtacct atcttaatca
    14881 tcatcttcct tgcggtactg acctggaaaa ccactcacgc tcttgagcct agcaagccgc
    14941 tggcacacga cgagaagccc attaccatcg aagtggtttc catggactgg aaatggttct
    15001 tcatctaccc ggaacagggc attgctaccg tgaatgaaat cgctttcccg gcgaacactc
    15061 cggtgtactt caaagtgacc tccaactccg tgatgaactc cttcttcatt ccgcgtctgg
    15121 gtagccagat ttatgccatg gccggtatgc agactcgcct gcatctgatc gccaacgaac
    15181 ccggtactta tgacggtatc tccgccagct acagcggccc gggcttctca ggcatgaagt
    15241 tcaaagctat tgcaacaccg gatcgcgccg cattcgacca atgggtcgca aaagcgaaac
    15301 agtcgccgaa ctccatgtct gacatggcag cgttcgaaaa actggccgcg cctagcgaat
    15361 acaaccaggt ggaatatttc tccaacgtga aaccagactt gtttgccgat gtgattaaca
    15421 agtttatggc tcacggtaag agcatggaca tgacccagcc agaaggtgag cacagcgcac
    15481 acgaaggtat ggaaggcatg gacatgagcc acgcggaatc cgcccattaa aggggttgag
    15541 gaagaataaa gatgttcgga aaattatcac ttgatgcagt cccgttccat gaacctatcg
    15601 tcatggttac gatcgctggc attattttgg gaggtctggc gctcgttggc ctgatcactt
    15661 acttcggtaa gtggacctac ctgtggaaag agtggctgac ctccgtcgac cataaacgcc
    15721 tcggtatcat gtatatcatc gtggcgattg tgatgttgct gcgtggtttt gctgacgcca
    15781 ttatgatgcg tagccagcag gctcttgcct cggcgggcga agcgggcttc ctgccacctc
    15841 accactacga tcagatcttt accgcgcacg gcgtgattat gatcttcttc gtggcgatgc
    15901 ctttcgttat cggtctgatg aacctggtgg ttccgctgca gattggcgcg cgtgacgttg
    15961 cgttcccgtt cctcaacaac ttaagcttct ggtttaccgt tgttggtgtg attctggtta
    16021 acgtttctct cggcgtgggc gaatttgcgc agaccggctg gctggcctat ccaccgctat
    16081 cgggaataga gtacagtccg ggagtcggtg tcgattactg gatatggagt ctccagctat
    16141 ccggtatagg tacgacgctt accggtatca acttcttcgt taccattctg aagatgcgcg
    16201 caccgggcat gaccatgttc aagatgccag tatttacctg ggcatcactg tgcgcgaacg
    16261 tactgattat tgcttccttc ccaattctga cggttaccgt cgcgttgttg accctggatc
    16321 gctatctggg cacccatttc tttaccaacg atatgggtgg caacatgatg atgtatatca
    16381 acctgatttg ggcctggggc cacccggaag tttacatcct gatcctgcct gttttcggtg
    16441 tgttctccga aattgcggca accttctcgc gtaaacgtct gtttggttat acctcgctgg
    16501 tatgggcaac cgtctgtatc accgtgctgt cgttcatcgt ctggctgcac cacttcttta
    16561 cgatgggtgc gggcgcgaac gtaaacgcct tctttggtat caccaccatg attatcgcca
    16621 tcccgaccgg ggtgaagatc ttcaactggc tgttcaccat gtatcagggc cgcatcgtgt
    16681 tccattctgc gatgctgtgg actatcggtt ttatcgtcac cttctcggtg ggcggtatga
    16741 ctggcgtgct gctggcagta ccgggcgcgg acttcgttct gcataacagt ctgttcctga
    16801 ttgcgcactt ccataacgtg atcatcggcg gcgtggtctt cggctgcttc gcagggatga
    16861 cctactggtg gcctaaagcg ttcggtttca aactgaacga aacctggggt aaacgcgcgt
    16921 tctggttctg gatcatcggc ttcttcgttg cctttatgcc actgtatgcg ctgggcttca
    16981 tgggtatgac ccgccgtttg agccagcaga ttgacccgca gttccacacc atgctgatga
    17041 ttgcagccag cggtgcagta ctgattgcgc tgggtattct ctgcctcgtt attcagatgt
    17101 acgtttctat tcgtgaccgc gaccagaacc gtgacctgac tggcgacccg tggggtggcc
    17161 gtacgctgga gtgggcaacc tcttccccgc ctccgttcta taactttgcc gttgtgccgc
    17221 acgttcacga gcgtgatgca ttctgggaaa tgaaagagaa aggcgaagcg tacaaaaagc
    17281 ctgaccacta tgaagaaatt catatgccga aaaacagcgg tgcaggtatc gtcattgcag
    17341 ctttctccac catcttcggt ttcgccatga tctggcatat ctggtggctg gcgattgttg
    17401 gcttcgcagg catgatcatc acctggatcg tgaaaagctt cgacgaggac gtggattact
    17461 acgtgccggt ggcagaaatc gaaaaactgg aaaaccagca tttcgatgag attactaagg
    17521 cagggctgaa aaatggcaac tgatactttg acgcacgcga ctgcccacgc gcacgaacac
    17581 gggcaccacg atgcaggcgg aaccaaaatc ttcggatttt ggatctacct gatgagcgac
    17641 tgcattctgt tctctatctt gtttgctacc tatgccgttc tggtgaacgg caccgcaggc
    17701 ggcccgacag gtaaggacat tttcgaactg ccgttcgttc tggttgaaac tttcttgctg
    17761 ttgttcagct ccatcaccta cggcatggcg gctatcgcca tgtacaaaaa caacaaaagc
    17821 caggttatct cctggctggc gttgacctgg ttgtttggcg ccggatttat cgggatggaa
    17881 atctacgaat tccatcacct gattgttaac ggcatgggtc cggatcgcag cggcttcctg
    17941 tcagcgttct ttgcgctggt cggcacgcac ggtctgcacg tcacttccgg tcttatctgg
    18001 atggcggtgc tgatggtaca aatcgctcgt cgcggcctga ccagcactaa ccgtacccgc
    18061 atcatgtgcc tgagcctgtt ctggcacttc ctggatgtgg tttggatctg tgtgttcact
    18121 gttgtttatc tgatgggggc gatgtaatga gtcattctac cgatcacagc ggcgcgtccc
    18181 atggcagcgt aaaaacctac atgacaggct ttatcctgtc gatcattctg acggtgattc
    18241 cgttctggat ggtgatgaca ggggctgcct ctccggccgt aattctggga acaatcctgg
    18301 caatggcagt ggtacaggtt ctggtgcatc tggtgtgctt cctgcacatg aataccaaat
    18361 cagatgaagg ctggaacatg acggcgtttg tcttcaccgt gctaatcatc gctatcctgg
    18421 ttgtaggctc catctggatt atgtggaacc tcaactacaa catgatgatg cactaagagc
    18481 ggcggttatg atgtttaagc aatacctgca agtaacgaaa ccaggcatca tctttggcaa
    18541 cctgatctcg gtgattgggg gattcctgct ggcctcaaag ggcagcattg attatcccct
    18601 gtttatctac acgctggttg gggtgtcact ggttgtggcg tcgggttgtg tgtttaacaa
    18661 ctacatcgac agggatatcg acagaaagat ggaaaggacg aagaatcggg tactggttaa
    18721 aggcctgatc tctcctgctg tctcgctggt gtacgccacc ttgctaggct ttgctggctt
    18781 tatgctgctg tggtttggcg cgaatccgct ggcctgctgg ctgggggtga tgggctttgt
    18841 cgtttatgtc ggcgtttata gcctgtacat gaaacgccac tcagtctacg gcacattgat
    18901 tggttcgctc tccggcgctg cgccgccggt gattggctac tgtgcagtaa ccggcgagtt
    18961 cgatagcggc gcagcgatcc tgctggctat cttcagcctg tggcagatgc ctcactccta
    19021 tgccatcgcc attttccgct ttaaggatta tcaggcggcg aacattccgg tattgccagt
    19081 ggtaaaaggc atttcggtgg cgaagaatca catcacgctg tatatcatcg cctttgccgt
    19141 tgccacgctg atgctctctc ttggcggtta cgctggatat aaatatctgg tggtcgccgc
    19201 ggcggttagc gtctggtggt taggtatggc tctgcgcggt tataaagttg ctgatgacag
    19261 aatctgggcg cgcaagctgt tcggcttctc tatcatcgcc atcactgccc tctcggtgat
    19321 gatgtccgtt gattttatgg taccggactc gcatacgctg ctggctgctg tgtggtaaca
    19381 aaacctctca gttaaaaagg tgctacggca ccttttttct tagcattaga aacatatccc
    19441 tctcgaaata tttactaaaa aatccgcatg tttaccccat tcgtttgccg ctttacacta
    19501 gtcgcgaatt taaaacagag gtggtaatga acgattataa aatgacgcca ggtgagcggc
    19561 gcgcgacctg gggtttagga accgttttct cgttgcgcat gctgggcatg tttatggttc
    19621 tgccggttct gaccacgtat ggcatggctc tgcaaggtgc cagcgaagca ttaatcggta
    19681 ttgccattgg tatttatggt ctgactcagg ccgtttttca gattccgttt ggcctgcttt
    19741 ccgaccgcat tggtcgcaaa ccattaattg tcggtgggct ggcggtgttt gccgccggta
    19801 gcgttatcgc tgcgctttct gactccatct ggggaattat tctgggccgg gcgctacaag
    19861 gctccggtgc gattgccgct gccgttatgg cgctgctttc cgatctcacg cgcgaacaaa
    19921 accgtaccaa agcaatggcg tttatcggcg tgagctttgg cattaccttt gccattgcga
    19981 tggtgcttgg cccgatcatc actcacaaac ttgggctgca cgcgctgttc tggatgatcg
    20041 ctattctggc aacaaccggc attgcgttga ccatttgggt tgtgcccaac agtagcactc
    20101 acgtacttaa tcgtgagtcc ggaatggtga aaggcagttt cagtaaagtg ctggcggaac
    20161 cgcggctgct aaaactcaac tttggcatta tgtgtctgca catgcttctg atgtctacgt
    20221 ttgttgccct gcccggacag ctagctgatg cggggttccc ggcggctgaa cactggaagg
    20281 tctatctggc gacgatgtta atcgcctttg gctcggtcgt gcctttcatt atctacgctg
    20341 aagttaagcg caaaatgaag caagtctttg tcttctgcgt cgggttgatc gtggttgcgg
    20401 aaattgtgtt gtggaacgca caaacgcagt tctggcaact ggtggtcggc gtgcagcttt
    20461 tctttgtggc gtttaatttg atggaagccc ttctgccctc acttatcagt aaagagtcgc
    20521 cagcaggtta caaaggtacg gcgatgggtg tctactccac cagccagttt cttggcgtgg
    20581 cgattggcgg ttcgctgggc ggctggattg acggcatgtt tgacggtcag ggggtatttc
    20641 tcgctggcgc aatgctggcc gcagtgtggc tggcagtcgc cagtaccatg aaagaaccgc
    20701 cgtatgtcag cagtttgcgc attgaaatcc cggcggacat tgccgcaaac gaagcgttaa
    20761 aagtgcgttt gctggaaact gaaggcgtca aagaagtgtt gattgcagaa gaagagcatt
    20821 cagcttatgt gaaaatcgac agcaaagtga caaatcgctt tgaggttgaa caggcaattc
    20881 gtcaggcata aaaaacgcgg cgaattgcct gctgcgctac gcttaccagg cctacaaagt
    20941 tcttgagtta caaaccgttg taggccgaat caggcgttca tgccgcatct gacatgaaca
    21001 aaacgcacat tgtcgcgatt aatcgcggaa gtttttgaac tggaacggct ggccgagatc
    21061 gccaccacgt accatcgcca tgacagcctg caaatcatca cgagatttgc cagtcacacg
    21121 gatctcatcg ccctgaattt gcgcctgcac tttcagtttg ctgtctttaa tcatcttgac
    21181 gattttcttc tgggtcgcgc tctcaatgcc ctgtttcagt ttcgcttcca caaaccaggt
    21241 tttaccgcta tgaacgatat tttccggtac atccagtgaa ctgccttcaa tgccgcgctt
    21301 cagcagcttg gcacgcagaa tatccagcaa ctgattgacc tggaagtcgg actcgctcaa
    21361 cactttgatg gttttgctgg cgtcgttcag ctcaaatgag gcttcaacgt tacggaagtc
    21421 aaaacgggac tccacttcgc gactcgcgtt atcgaccgcg ttacgtgctt cctgaagatc
    21481 aacttcagag acaatatcga aagatggcat cttttcctct cccttcattt ttgatgcgaa
    21541 gcataatacc tgcaaagtta aataaccaac agtcctgcac caggacgtat cccttaattg
    21601 tgcatagtcg caacagcatg cacaattaag ggatagcctt attgaacacc cggagtggtt
    21661 gcgggtgagg aggaacaatg aaaattaccg tattgggatg cggtgcctta gggcaattat
    21721 ggcttacagc actttgtaaa cagggtcacg aagttcaggg ctggctgcgc gtaccgcaac
    21781 cttattgtag tgtgaatctg gttgagacag atggttcgat atttaacgaa tcgctgaccg
    21841 ccaacgatcc cgattttctc gccaccagcg atctgctcct ggtgacgctg aaagcatggc
    21901 aggtttccga tgccgtcaaa agcctcgcat ccacactgcc tgtagctacg ccaatactgt
    21961 taattcacaa cggcatgggc accatcgaag aattgcaaaa cattcagcag ccattactga
    22021 tgggcaccac cacccatgcc gcccgccgcg acggcaatgt cattattcat gtggcaaacg
    22081 gaatcacgca tatcggtccg gcacggcaac aggatggcga ttacagttat ctggcggata
    22141 ttttgcaaac cgtgttgcct gacgttgcct ggcataacaa tattcgcgcc gagctgtggc
    22201 gcaagctggc cgtcaactgt gtgattaatc cactgaccgc catctggaat tgcccgaatg
    22261 gtgaattacg tcatcatccg caagaaatta tgcagatatg cgaagaagtc gcggcagtga
    22321 tcgagcgcga agggcatcat acttcagcag aagatttgcg tgattacgtg atgcaggtga
    22381 ttgatgccac agcggaaaat atctcgtcga tgttgcagga tatccgcgcg ctgcgccaca
    22441 ccgaaatcga ctatatcaat ggttttctct tacgccgcgc ccgcgcgcat gggattgccg
    22501 taccggaaaa cacccgcctg tttgaaatgg taaaaagaaa ggagagtgaa tatgagcgca
    22561 tcggcactgg tttgcctcgc ccctggtagt gaagagactg aagccgtcac cactatcgat
    22621 ctgctggttc gcggcggtat caaagtcacc actgccagcg tcgccagcga tggcaacctg
    22681 gccattacct gctcgcgcgg cgtgaagctg ctggcggatg cgccgctggt cgaggtggct
    22741 gatggcgaat atgacgtgat cgtactgcct ggtggcatta aaggcgcaga gtgttttcgc
    22801 gatagtaccc tgctggttga aaccgttaaa cagttccacc gttccgggcg tatcgtcgcg
    22861 gctatttgcg ccgcgccagc caccgtgctg gtgccgcacg atatcttccc gattggtaat
    22921 atgaccggct tcccgacgct gaaagacaaa attcccgctg aacaatggca ggacaagcgc
    22981 gtcgtctggg atgcacgggt aaaattgctg accagccagg ggccggggac agctatcgac
    23041 tttggtctga aaattatcga cctgctggtt gggcgcgaga aagcccatga agtggcatca
    23101 caactggtga tggcggcagg gatttataat tattacgagt agtgtcggat gcggtaagcg
    23161 tcgcatctga ccagatgcga cgttacaacc acaaattacg ggcgatatac cttcacattg
    23221 ttaaagccct gctcgcgcag atagagcgcc tgcagacggc tcatcacccc gcgctcacac
    23281 cacagtagcc aggttctgtt ctggtcgaga tcgccaaact tggtgctcag tttatagaac
    23341 ggcagagaaa ctacatcaat cccttcgact ttcagtggct tatcttcctg ttcatcgata
    23401 gaacggatat cgaggatcac gtcgttcggg ccgaagccat tgacggtttc cacttccacc
    23461 acttcctgct cggtctgctg ggcgatttcg cggatatcaa cgttattcgc ttcttcaacc
    23521 actttatcga gaatgctgaa gtcgaatttc tcttcttccg cttcaatctt cgacttaacc
    23581 gctttcaccg tcgggctttt ggagataaca ccgcaatatt ccggcatcgt gcgagcaaag
    23641 tcttcggtgc caatctggcg agccaggttg atgatgtgct ctttgtcgta agaaatcagc
    23701 ggacgcagga tcagcgtatc ggagacgtta tcaatcaggc gcaggttggt cagcgtctga
    23761 ctggacacct ggccgagcgc ttcgccggtg accagcgcct gtacgccgta acgttcagcc
    23821 actttagacg cggcacgcac catcatacgt ttgaggataa cgcccatctg accgtcgtcg
    23881 attttctcga gaatttcccc gacgaccggt tcgaaattaa tagcgacaaa acgcacgcgg
    23941 tgggagctgc caaaacggtt ccacagataa tgcgccacct gacgcacgcc aatttcatgc
    24001 gccgcgccgc caaggttaaa gaaacagtaa tgcacgcggc agccgcgacg catcaacata
    24061 taactggaaa caccggagtc gaaaccaccg gaaatgagcg acagcacatc ttcctgggtg
    24121 ccgatcggga aaccgccaat accttcgtag cggcctttaa tcagcaggag acgatcgtct
    24181 tccacttcca gatggacagt cacttccgga ttggtcagct tcacgcgcgc ggactcaata
    24241 tgctgattta aaccgccgcc gacgtaacgt tccacatcaa tcgagctaaa atcatgtttg
    24301 ccacggcgct tcacgcgtac gcagaaggtt ttgccttcca gctgatcgcg atactgaacc
    24361 aacgctttct caaaaatatc gtgcatgtcg gtaaacggca cgtcttcgac ttcgagaata
    24421 tggtggatac ccggaatacg ggtcagggcg tcgcgaatag ccagacgctg gttttcatct
    24481 tttgcgcgaa cttcgatgtt atcccagtgg cggacgacag cgagcgtctc atcatagtgc
    24541 tttaaaacgt tacgaatgtt cccggtaagg atttttataa agcgcaagcg cacagattgg
    24601 cttttgatgg tgatttccgg gaacaattta atgataaact tcatggcggc aatggttcgt
    24661 tggcaagcct taagcgactt gtatagggaa aaatacagca gcccacacct gcggctgcat
    24721 ccaggcgcgg aagtatacca ctaacatcgc tttgctgcgc acatcacctt accattgcgc
    24781 gttatttgct atttgccctg agtccgttac catgacgggg cgaaaaatat tgagagtcag
    24841 acattcatta tgccgaagaa aaatgaggct cccgccagct ttgaaaaggc gctgagcgag
    24901 ctggaacaga ttgtaacccg tctggaaagt ggcgacctgc cgctggaaga ggcgctgaac
    24961 gagttcgaac gcggcgtgca gctggcacgt caggggcagg ccaaattaca acaagccgaa
    25021 cagcgcgtac aaattctgct gtctgacaat gaagacgcct ctctaacccc ttttacaccg
    25081 gacaatgagt aatggacttt ccgcagcaac tcgaagcctg cgttaagcag gccaaccagg
    25141 cgctgagccg ttttatcgcc ccactgccct ttcagaacac tcccgtggtc gaaaccatgc
    25201 agtatggcgc attattaggt ggtaagcgtc tgcgaccctt cctggtttat gccaccggtc
    25261 atatgtttgg cgttagcaca aacacgctgg acgcacccgc tgccgccgta gagtgtatcc
    25321 acgcttactc attaattcat gatgatttac cggcgatgga tgatgacgat ctgcgtcgcg
    25381 gtttgccgac ctgccatgtg aagtttggcg aagcaaacgc gattctcgct ggcgacgctt
    25441 tacaaacgct ggcgttctcg attctaagcg atgccaatat gccggaagtg tcggatcgcg
    25501 acagaatttc gatgatttct gaactggcga gcgccagcgg tattgccgga atgtgcggtg
    25561 gtcaggcatt agatttagac gcggaaggca aacacgtacc tctggacgcg cttgagcgta
    25621 ttcatcgtca taaaaccggc gcattgattc gcgccgccgt tcgccttggt gcattaagcg
    25681 ccggagataa agggcgtcgt gctctgccag tactcgacaa gtacgcagag agcatcggcc
    25741 ttgccttcca ggttcaggat gacatcctgg atgtggtagg agatactgca acgttgggaa
    25801 aacgccaggg tgccgaccag caacttggta aaagtaccta ccctgcactt ctgggtcttg
    25861 agcaagcccg gaagaaagcc cgggatctga tcgacgatgc ccgccagtcg ctgaaacaac
    25921 tggctgaaca atcactcgat acctcggcac tggaagcgct agcggactac atcatccagc
    25981 gtaataaata aacaataagt attaataggc ccctgatgag ttttgatatt gccaaatacc
    26041 cgaccctggc actggtcgac tccacccagg agttacgact gttgccgaaa gagagtttac
    26101 cgaaactctg cgacgaactg cgccgctatt tactcgacag cgtgagccgt tccagcgggc
    26161 acttcgcctc cgggctgggc acggtcgaac tgaccgtggc gctgcactat gtctacaaca
    26221 ccccgtttga ccaattgatt tgggatgtgg ggcatcaggc ttatccgcat aaaattttga
    26281 ccggacgccg cgacaaaatc ggcaccatcc gtcagaaagg cggcctgcac ccgttcccgt
    26341 ggcgcggcga aagcgaatat gacgtattaa gcgtcgggca ttcatcaacc tccatcagtg
    26401 ccggaattgg tattgcggtt gctgccgaga aagaaggcaa aaatcgccgc accgtctgtg
    26461 tcattggcga tggcgcgatt accgctggca tggcgtttga agcgatgaat cacgcgggcg
    26521 atatccgtcc tgatatgctg gtggtcctca acgacaatga aatgtcgatt tccgaaaatg
    26581 tcggcgcgct caataaccat ctggcacagc tgctttccgg taagctttac tcttcgctgc
    26641 gcgaaggcgg gaaaaaagtt ttctctggcg ttccgccaat taaagagctg ctcaaacgta
    26701 ccgaagaaca tattaaaggc atggtagtgc ctggcacgtt gtttgaagag ctgggcttta
    26761 actacatcgg cccggttgac ggtcacgatg tgctggggct tatcaccacg ctgaagaaca
    26821 tgcgcgacct gaaaggcccg cagttcctgc atatcatgac caaaaaaggt cgtggttatg
    26881 aaccggcaga aaaagacccc atcactttcc acgccgtgcc taaatttgat ccctccagcg
    26941 gttgtttgcc gaaaagtagc ggcggtttgc cgagctattc aaaaatcttt ggcgactggt
    27001 tgtgcgaaac ggcagcgaaa gacaacaagc tgatggcgat tactccggcg atgcgtgaag
    27061 gttccggcat ggtcgagttt tcacgtaaat tcccggatcg ttacttcgac gtggcaatcg
    27121 ccgagcaaca cgcggtgacc tttgccgccg gtctggcgat tggtgggtac aaacccattg
    27181 tcgcgattta ctccactttc ctgcaacgcg cctatgatca ggtgctgcat gacgtggcga
    27241 ttcaaaagct cccggtcctg ttcgccatcg accgcgcggg cattgttggt gctgacggtc
    27301 aaacccatca gggcgctttt gacctctctt acctgcgctg tataccggaa atggtcatta
    27361 tgaccccgag cgatgaaaac gaatgtcgcc agatgctcta taccggctat cactataacg
    27421 acggcccgtc cgcggtgcgc tacccgcgcg gtaacgcggt tggcgtggaa ctgacgccgc
    27481 tggaaaaact gccaattggc aaaggcattg tgaagcgtcg tggtgagaaa ctggcgatcc
    27541 ttaactttgg tacgctgatg ccagaagcgg cgaaagtcgc cgaatcgctg aacgccacgc
    27601 tggtcgatat gcgttttgtg aaaccgcttg atgaagcgtt aattctggaa atggccgcca
    27661 gccatgaagc gctggtcacc gtagaagaaa acgccattat gggcggcgca ggtagcggag
    27721 tgaacgaagt gctgatggcc catcgtaaac cagtacccgt gctgaacatt ggtctgccgg
    27781 acttctttat tccgcaagga actcaggaag aaatgcgcgc cgaactcggc ctcgatgccg
    27841 ccggtatgga agccaaaatc aaggcctggc tggcataatc cctactccac tcctgctatg
    27901 cttaacaaat tattcataga ctctaaataa ttcgagttgc aggaaggcgg caaacgagtg
    27961 aagccccagg agcttacata agtaagtgac tggggtgaac gaatgcagcc gcagcacatg
    28021 caacttgaag tatgacgagt atggcaggag tggcagcatg caatacaacc ccttaggaaa
    28081 aaccgacctt cgcgtttccc gactttgcct cggctgtatg acctttggcg agccagatcg
    28141 tggtaatcac gcatggacac tgccggaaga gagtagccgt cccatcatta aacgcgcgct
    28201 ggaaggcggc ataaatttct ttgataccgc caatagcttt tccgatggca gcagcgaaga
    28261 gatcgttggt cgcgcactgc gggatttcgc ccgtcgtgaa gacgtggtcg ttgcgacgaa
    28321 agtgttccat cgcgttggtg atttaccgga aggattatcc cgtgcacaaa ttttgcgctc
    28381 tatcgacgac agcctgcgcc gtctcggcat ggattatgtc gatatcctgc aaattcatcg
    28441 ctgggattac aacacgccga tcgaagagac gctggaagcc ctgaacgacg tggtaaaagc
    28501 cgggaaagcg cgttatatcg gcgcgtcatc catgcacgct tcgcagtttg ctcaggccct
    28561 gaagctacaa aaacagcacg gctgggcgca gtttatcagt atgcaggatc actacaatct
    28621 gatttatcgc gaagaagagc gcgagatgct gcctctgtgt tatcaggagg gcgtggcggt
    28681 gattccgtgg agcccgctgg cgcgggggcg actgacgcgt ccgtggggag aaactaccgc
    28741 acgactggtg tctgatgagg tggggagaaa tctctataaa gaaagcgatg aaaatgacgc
    28801 gcagatcgca gagcggttaa cgggcgtcag tgaagaactc ggtgcaacac gagcacaagt
    28861 tgcgctggcc tggttgttga gtaaaccggg cattgccgca ccgattatcg gtacatcgcg
    28921 ggaagaacag cttgatgagc tgctgaacgc ggtggatatc actttgaagc cagaacagat
    28981 tgccgaactg gaaacgccgt ataaaccgca tgcggtcgta ggatttaaat aagagtccag
    29041 gcctgatgag acgtgacaag cgtcacatca ggcatcggtg cacaactacg acagaatacc
    29101 cagcggccag tgatgaccga taaagtacag gatgcctgcg gaaatcaccc cggcgacaat
    29161 atcgtcgatc atgatcccca tgccgccatg tacattgcga tcaaaccagc ggatcggcca
    29221 cggcttccac atatccagaa tacggaaaat cacaaacccg gcggcaaccc actgccagtc
    29281 attggtcggc agcgccatga gcgtgatcca cataccaata aattcgtccc agacaatgct
    29341 gccgtgatcg tgcacgccca tatctttcgc cgtttgatga caaagataga cgccgataca
    29401 gatccccaac atcaccacca gcgagtagag ctgccagggt aaaaaggtca tcagatacca
    29461 gaacggaatc gctgccagcg atcccatcgt gccaggaacg attgggctta atccacttcc
    29521 gaatccgaca gcaagcagat gccacggatt actcatcttc aggcgacttt tcgcgacatc
    29581 tttatggcgt ggcaaaatgg tcatatcctt tccagtcaaa cgtgacaggt tcgccgtcac
    29641 gaataaaaca aagcccttcg atatcggcgg tcatttgccc gatacaggta aacggtacgc
    29701 ccaggtgtcc gagagccaca tccagcgcgc cacggttcag ttccggcaca gtgaaacaca
    29761 actcgtaatc ttcaccgcca gagagcgccc agcgcagcgc ctgttccggt tcaacatggc
    29821 gagaaagcgc atcagaaaac ggcagcaatg ccaggtcaat acgtgcgccg cagtcgctgg
    29881 ctttcacgat atgcccgaga tcggaaatca gaccgtcaga gagatcgata gctgaatttg
    29941 ccagatcgcg cagtgcctgt ccctgtaaaa tacgcggcga tggacggaga tgacgtttga
    30001 tcaagtagtc cgcatcttta gcatcggcaa cctgcaaacg gttttgcaaa atcgccagcc
    30061 cggcggcgct atcgcccggt gtaccggtca catagatcca gtcacccggt ttcgctccag
    30121 agcgcgttaa ggctcgtccc atcggaacaa agccgtggat acccaacgtc attgataatg
    30181 gcccacgcgt ggtatcgccg ccaatgagtt gcatatcgta ataattgaga agatcaaaca
    30241 aactgtcgct gaaggactca agccacgctt cgtctacgtc cggtaaggtt aatgccagcg
    30301 tcagccaggc cggatcggcc cccatcgctg ccagatcgct taggttcacc gccagtgctt
    30361 tatacgccag atcagcagga tcgatatcat ggaggaagtg gttgcccgcc accagcgtat
    30421 cagtgctgat cgccagggtc tgtttctcgg ggatattgag aagtgcgcaa tcgtcgccga
    30481 tgcccagttc gacatcaaga cgagaacttc ttacacggtc aaaataacgg gcaatcaggg
    30541 agaactcgcc acatgccata cgttatgcct cagcaggtaa aaaagaaaag gccggcgatc
    30601 atggaacggt cttccgtgaa tctaccggcc tggatattac tttttgttag ggcgaatcac
    30661 aggtgctgct ttatcgagta cgccgttgac gaacttatgg ctgtcttctg cgccgaacga
    30721 tttcgccagt tcgatcgctt cgttaatggc cactttgtat ggcacatcgc tacgtttaga
    30781 cagctcgtac agcgcaatgc gcagtactgc tttttctacc tgtcccagtt cttccagcag
    30841 gcgggacagg tatggcttca tcagtccgtc gaggtatgcg gtgttagtcg ccaccccggc
    30901 cagcagctca cggaagtaca ggacgtcaac atcttttaca tcctgttcag ccaggaactg
    30961 gtattcaaca tcagcgatgt cgttctggga caactgccag gagtagagcg cctggacggc
    31021 acactcacga gcgcggcgac gagcagcagg tttcacggat ttccccttac taatttcagg
    31081 ccttgatggc tttcaataca ttaatcattt caagcgcggt cagtgcagct tctgcacctt
    31141 tgttgccagc tttggtgcca gcacgttcga tcgcttgttc aatgctttca gtggtcagaa
    31201 ccccaaaagc aaccggaatt tcgctgtcct gggcaacatg cgccaggccg ttgcttgcac
    31261 caccagcgac atattcaaaa tgggcagtgc caccacgaat aaccgtaccc agcgcaatca
    31321 ccgcgtcgta tttaccggtt ttagccagtg cacccgccgc cagcggcagc tcataggcac
    31381 caggcaccca aacaacggta atgttttcat cttttacctg accgatacgt ttcagtgcgt
    31441 caattgcacc ttccagcagg ctgtcattga taaagttgtt gaaacgcgcg atggtgatgg
    31501 cgacgcgagc gtccggggta gcaacgttag cttcaataat gttcattttc ttccttcggg
    31561 ttcgagtatg gccccgcagg ggggcggatt ttagcataat atttcgtgcg ctgcttccct
    31621 ttcgagccgg gagatcatgc acccactaaa tgcaggcaaa catccgggcc tacatgacgt
    31681 atctctttga atttaaattg gggggcgtcg gctaatttct caagccctgg cagcgtgcat
    31741 aatccgcggg cgtcgctgcc taatagttta ggtgcgatat agacaatcag ctcatcgact
    31801 aaacccgcct gcagcaatgc gccagcgagc gttggccccg cttccaccca gatgctgtta
    31861 atttgctgtt tacccagttg catcatcagt acaaccagat ccagatgacc tttatgctct
    31921 ggaatcagca aggtacgcac cgtttccggc cactcactag aatcttcctg cgtacgcgcg
    31981 aaccaggttt cgccgggctg ctgcacaatg cgatgttccg gcgtcacgcg attttggcta
    32041 tcaatcacaa tacgtactgg ctggcggaga ttttgttgcg ggtagagcgc ctgagtttgt
    32101 tcatccagtt cagaccaacg cactgttaag gctggatcat ccgccagcac cgtggcgctg
    32161 ctggttaaaa tggcatgact ttgtgcgcgc tgtcgttgta cgtcgcgccg cgcctgaggc
    32221 gaagtgatcc actggctttc gccgctcgcc atcgccgtgc ggccatcaag cgatgcgcca
    32281 agttttaact gaatataagg aaagccggtg cgcatccgct tgagaaagcc tttattcaat
    32341 tgctcggctt cactcatcat cagcccgtgg ctgacgtcaa tgccagcctg ttgcagacgg
    32401 taaagtccac gcccagcgac ctgcgggtta ggatcttgca tcgaggcaac cacgcgcgct
    32461 acgccagcgg cgatgagtgc gtcacagcac ggtggcgtac gaccatgatg gctacagggt
    32521 tcgagtgtga cataggcggt cgcacctttg gctttttcac ccgccatacg caacgcgtgt
    32581 acttcggcat gtggttcacc cgcacgttgg tggtaacctt caccgacaat ttcgccatct
    32641 ttgacaatga cgcacccgac attcgggttg ggatgcgtgg taaaacgtcc tcgttgcgcc
    32701 agctttagcg cccgcgccat gtaatactcg tcctgcacgg cttagtcctc caggcgcgcg
    32761 atctcttcgc caaattcttt gatatcttcg aaactgcggt agacagaggc aaaacggata
    32821 taggcgactt tatcgagctt tttcaattgc tccatcacca gattgccaat catcttgctc
    32881 ggcacttcgc gctcaccggt ggcgcgcagc tgcgatttaa tatgattgat tgccatttcg
    32941 acgtcatcgg aactcaccgg acgtttttcc agcgcccgca gcattccgct acgcaatttc
    33001 tcttcattaa acggttcacg cacgtcgttg ctttttacaa cacgcggcat aaccagctcc
    33061 gccacttcaa aggtggtgaa acgttcatta cacaccagac actgccgacg gcggcgtacg
    33121 gatgaaccct cgcccacgag acgagagtca attaccttag tgtccacggc gaaacagaat
    33181 gggcaatgca tacggcgtcc tgaccaggtg gttaacagaa atctatttta ccctgaactg
    33241 acgatacaac aaaggcgcaa cgcttttgag ataaaggatc gatgctttcg ctgcttttac
    33301 cctctaccat taggctaatt gcgacgagaa agggacgacc tatgaatact aacgtttttc
    33361 gactgctcct gctgggaagc ctgttcagcc ttagcgcctg tgtgcagcag agcgaagtgc
    33421 gacagatgaa acacagcgtc agcacgctga accaagagat gacgcaactc aataaagaaa
    33481 cggtcaaaat cactcagcaa aacaggctga atgcaaaatc cagcagtggg gtttaccttc
    33541 tgccaggagc gaaaacaccg gcaagactgg aaagccagat cggtacttta cgtatgtcgc
    33601 tggtgaatat tacgcctgat gcagatggca ccaccctgac gctacgtatt cagggcgagt
    33661 cgaatgatcc cttgcccgca ttcagcggaa ctgttgaata tgggcaaatt cagggaacaa
    33721 tagacaactt tcaggaaatc aatgtgcaga atcaattgat taatgcccct gccagcgtcc
    33781 tcgcccccag cgatgttgat attccgttac agttaaaggg tatttctgta gaacagttag
    33841 acttcgtacg tatccacgat attcaacctg ttatgcagta aacgtatttc ggggcgattt
    33901 tgtgcgtctc gcaacatctt tccccgtcat tttgttactc cgcttacatc accctgattg
    33961 atagtaaaag tttgcaacaa gggtgaaagt cagtacaatc cctgcccgaa tgtgtgtaaa
    34021 cgtgaacgca atcgattacg taaatgatag aactgtgaaa cgaaacatat ttttgtgagc
    34081 aatgattttt ataataggct cctctgtata cgaaatattt agaaacgcaa tttgcacctt
    34141 tttcactccc gcaagggatt ttcaaacagt ggcatacata tgaaaaaaac attactggca
    34201 gccggtgcgg tactggcgct ctcttcgtct tttactgtca acgcagctga aaacgacaaa
    34261 ccgcagtatc tttccgactg gtggcaccag agcgttaacg ttgtcggaag ctatcacacc
    34321 cgtttcggac cgcagatccg caacgatacc taccttgagt acgaagcatt cgctaaaaaa
    34381 gactggttcg acttctatgg ttatgcggat gcgccggtat tcttcggcgg taactccgat
    34441 gctaaaggta tctggaacca cggttctccg ctgtttatgg aaatcgaacc acgtttctcc
    34501 atcgacaagc tgaccaatac tgaccttagc ttcggtccgt tcaaagagtg gtacttcgcg
    34561 aacaactaca tttacgacat gggtcgtaat aaagatggtc gccagagcac ctggtacatg
    34621 ggtctgggta ccgacatcga tactggtctg ccgatgagcc tgtccatgaa cgtctatgcg
    34681 aaataccagt ggcagaacta tggcgcagcg aacgaaaacg agtgggacgg ttaccgtttc
    34741 aaaattaaat actttgtgcc gattaccgat ctgtggggcg gtcagctgag ctacatcggc
    34801 ttcaccaact tcgactgggg ttccgattta ggggatgaca gcggtaacgc aatcaacggt
    34861 attaagaccc gtactaataa ctctatcgct tccagccata ttctggctct gaactacgat
    34921 cactggcact actctgtcgt agctcgttac tggcacgacg gtggtcagtg gaacgacgat
    34981 gcagaactga acttcggcaa cggcaacttc aacgttcgct ctaccggctg gggtggttac
    35041 ctggttgtag gttacaactt ctgattatga aaatgccggg atttattccc ggcatttcag
    35101 attgttaaca acgtgcgctt tgttcatgcc ggatgcggcg taaacgcttt gtccgaccca
    35161 caaaagcacg taaattcaat atattgctga aataacgtag tcctgataag cgtagcgcat
    35221 caggcaattt tacgttttaa tcacttcttc ttgttcatca tcgctttcag gtcagcgaat
    35281 gggttgtact tcgcttcacc gacatctttc tgcgcatctt cccctgcgat aacggtcgta
    35341 ccatactgat ccgcttcggt atatttcgaa tgctcatgat cgtggcaata gagacaaagc
    35401 aattcccagt tactgccatc ttccgggtta ttggtatggt cgtgatcaat gtggtgaaca
    35461 gtaagttcac gcaggttgga ataaacaaac tcgcgggaac agcgaccgca gacccacgga
    35521 tatattttta atgctttttc gcgatagccg ctttctaacc gcgcatagtt ttttgggatg
    35581 atagccatga agagttacct ggatgaaatg tagggttacg cgcagtttcc caagatgcgg
    35641 ggattttcgc aatctccata catcaaaaaa aatcccgatc ttctgaccgg gattttcaac
    35701 atcaacggga acttgattac ggcagaattg acggctgatc cgccccttct ttttccactt
    35761 tctgctgcaa catgtgttcg cgcttcatac ccagtttcag agccaacgca gatgccacat
    35821 agatggaaga tgcagtaccg atggaaacac cgataagcat ggtcagcgag aagccttcca
    35881 gtaccggacc accgaagagg tacagcatca ggataaccat caaggtagta ccggatgtga
    35941 tcaaggtacg gtgcagcgtc tgggtcaagg acacgttaaa gatttcgtaa ggcgtaccgc
    36001 gacggatctt gcggaagttt tcacgaatac ggtccgatac cacgatactg tcgttaagcg
    36061 agtaaccgat aaccgacatc aacgatgcca caatggtcag gtcaatctcg atatggaata
    36121 acgacaaaat acccagcgta atgataacgt cgtgcgccag cgcaataaca acccctgccg
    36181 ccagtcgcca ctcaaagcga aaacctacgt acacaaggat agacagcagc gccgccatca
    36241 acgccatcgc accggtttgc gcaaggtctg cccccacgct cggaccgacg aactcaatac
    36301 gcttcactgc tgcattctga ttggtggatt cgttaatcac cttcagaacc tggctgccca
    36361 gcacctgacc gccggtttcg ccttcagcag gcggcatacg gaccatgatg tcgtggctgc
    36421 taccaaagtt ttgcagcatt ggctcttcaa aaccggcttt ttgcaatgca tcacgcatta
    36481 cgtcaatttc agccggtttt tcgagcgtaa tttcgataac cgtaccaccg gtgaaatcca
    36541 gcccccagtt aaagccgcgc acgcccataa taacgatagc ggcgattaac agcagaccag
    36601 agatgccgaa agcccagtag tcccagcgca taaagtcata gactttacgg ccgtggttta
    36661 gttgttcaac agtatattcc tgtgccacat cgcactcctc agattgacag cttcttgacg
    36721 cgcttgccgc catatagcag gtttacgatg gcacgcgtac cgacaatcgc ggtaaacatc
    36781 gacgtcgcca caccgatacc ggtagtaatc gcgaaccctt taattgcccc ggtacccact
    36841 gcgtacagga tgatgacttt aatcagcgtg gtgatgttcg catcgaagat agaactgaat
    36901 gcgccacgat aaccttcatc aattgcctgt tgaacagtac gtccgttgct caactcttct
    36961 ttaatacgtt cgttgatcag tacgttcgca tcgaccgcca ccgcaagggt taagacgata
    37021 cccgcgatac ctggcatact cagcgttgcg cctggcaaca gcgacataat gccgacgatt
    37081 aagatcaagt tggcaatcag agcactggtc gcaatcagac caaacttctt atagaagatg
    37141 atcatgaaca gaatagaaac cagcagaccg gcaaggcaag cctccagccc ctgttcaatg
    37201 ttctgcatac ccagggttgg gccaatggta cgttcttcaa caatctgaat cggcgcgatc
    37261 aacgcaccgg cacgcagcag cagtgacagc tgacgggctt cgttcgggtt gttgatgccg
    37321 gtgatacgga agctgttacc cagacgagac tggatgttgg cgatgttaat cacctcttcc
    37381 tgtttcacca gaaccgcacg accatttgca tctttcttac cgctgtcttt gtactccaca
    37441 aacagggttg ccatcggttt gccgatgttg tccttagtga agttagacat gatgttacca
    37501 ccagcgctat cgagcgagat gttaacctgc ggctggttgt attcgtcctg gctggaagtg
    37561 gagtcggtga tatggtcacc ggtcagaatt acgcgtttgt acagcacaac tggctgacct
    37621 tcgcgggtct gtttcacttc agagtcgccc ggtacgcgac cggatgccgc agcggcctgg
    37681 tcaacgttgg tgtttaccag acggaattcc agcgttgccg tcgcacccag aatctctttc
    37741 gcacgcgcag tgtcctgaat acctggcagt tcaacaacga tacggtcagc accctgacgc
    37801 tgaaccaccg gctccgccac gccaagttgg tttacacggt tacgcaggat attgatgttc
    37861 tgctgcaccg catattcacg cgcttcactc agacgagcat cgctcattac cgcacgcagc
    37921 tggttgctgc cctggctgct aattaccagg tccggatggc gcttgctcag atacgcaatg
    37981 gcttcatcac gagctttagc atcgcggaaa gtaatgctca ggccgtagtt gttttcttta
    38041 cgaacggtgg tatacgggat gcccttttca cgcagatcgc tgcgcaggct atcgatattt
    38101 tgttcctgga gtttgccaag cgcggtgtcc atatccactt ccatcaggaa gtgaacgccg
    38161 ccacgcaggt caaggccgag cttcatcggc tcagcgtgaa tagctgccag ccagcgcggc
    38221 gttgccgggg caaggttaag cgccacgacg tatttgtcac ccataacgcc cattaatgct
    38281 tcacgagcgc gcagctgggt gtcagtggag tcgaagcgcg caagaatagc gccctcttcc
    38341 agtgccacag acttagcagt tattttttct tcttgtaacg ttttctggac ctggatcagc
    38401 gtttgctcac tggcggcgac tccgcgcgca ccagtgatct gaacagccgg atcctcacca
    38461 aacaggttgg gaagcgcata cagcagaccg atgacaatca ccacgatcag catgacgtac
    38521 ttccacaaag gataacggtt taacacggca attcccttag ggaaaaattt taattacagc
    38581 gccttcatgg tgcctttcgg caggacggca gctacgaagt cacgtttaat aactacttca
    38641 gtggtgtcat tcagcgcgat agcaatgtag ccgttttccg ctactttggt tacgcgacca
    38701 accaggccac cgttcgtcag aacttcatca cctttggcaa tggagtccat cagctttttg
    38761 tgttctttgg tgcgcttctg ctgtggacgc aggatcatga aatagaaaat cagaccgaat
    38821 accaccagca tcaaaatcaa agacatcggg ctaccttgcg ccggtgcacc cgttgccgct
    38881 accgcatcag aaataaaaaa gctcattaaa tttccctcat tattaatatt aatcaacgtt
    38941 caaaggtggt acttctcgcc cctgacgctg gtaaaaatca gttacgaagc tctctaattt
    39001 accctcttca atagccttgc gtaaacccgc catcaaacgc tggtagta
//
LOCUS       DAMZET010000023        39007 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.023, whole genome shotgun sequence.
ACCESSION   DAMZET010000023 DAMZET010000000
VERSION     DAMZET010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.023"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..539
                     /locus_tag="POB34_001315"
     CDS             <1..539
                     /locus_tag="POB34_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5918300.1"
                     /translation="LPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAVVGLLSLA
                     YLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGMLLFSAGFF
                     AAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNGVGAFIAL
                     MLVIALLVGTRLHRRLHA"
     gene            936..1271
                     /locus_tag="POB34_001316"
     CDS             936..1271
                     /locus_tag="POB34_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HDI5918301.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQP
                     AKPAAQPAA"
     gene            1364..1447
                     /locus_tag="POB34_001317"
     CDS             1364..1447
                     /locus_tag="POB34_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918302.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            1547..2368
                     /locus_tag="POB34_001318"
     CDS             1547..2368
                     /locus_tag="POB34_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HDI5918303.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(2407..2736)
                     /gene="mdtI"
                     /locus_tag="POB34_001319"
     CDS             complement(2407..2736)
                     /gene="mdtI"
                     /locus_tag="POB34_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HDI5918304.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(2723..3088)
                     /gene="mdtJ"
                     /locus_tag="POB34_001320"
     CDS             complement(2723..3088)
                     /gene="mdtJ"
                     /locus_tag="POB34_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HDI5918305.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIVGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            3500..4534
                     /gene="tqsA"
                     /locus_tag="POB34_001321"
     CDS             3500..4534
                     /gene="tqsA"
                     /locus_tag="POB34_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HDI5918306.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(4559..5947)
                     /gene="pntB"
                     /locus_tag="POB34_001322"
     CDS             complement(4559..5947)
                     /gene="pntB"
                     /locus_tag="POB34_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HDI5918307.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5958..7490)
                     /gene="pntA"
                     /locus_tag="POB34_001323"
     CDS             complement(5958..7490)
                     /gene="pntA"
                     /locus_tag="POB34_001323"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HDI5918308.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCAYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            8014..8958
                     /locus_tag="POB34_001324"
     CDS             8014..8958
                     /locus_tag="POB34_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDI5918309.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            9144..10526
                     /locus_tag="POB34_001325"
     CDS             9144..10526
                     /locus_tag="POB34_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI5918310.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            10563..11285
                     /gene="folM"
                     /locus_tag="POB34_001326"
     CDS             10563..11285
                     /gene="folM"
                     /locus_tag="POB34_001326"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HDI5918311.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(11282..11617)
                     /locus_tag="POB34_001327"
     CDS             complement(11282..11617)
                     /locus_tag="POB34_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HDI5918312.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            11746..12465
                     /gene="rstA"
                     /locus_tag="POB34_001328"
     CDS             11746..12465
                     /gene="rstA"
                     /locus_tag="POB34_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HDI5918313.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            12469..13770
                     /gene="rstB"
                     /locus_tag="POB34_001329"
     CDS             12469..13770
                     /gene="rstB"
                     /locus_tag="POB34_001329"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HDI5918314.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            13846..14775
                     /gene="tus"
                     /locus_tag="POB34_001330"
     CDS             13846..14775
                     /gene="tus"
                     /locus_tag="POB34_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HDI5918315.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(14772..16175)
                     /gene="fumC"
                     /locus_tag="POB34_001331"
     CDS             complement(14772..16175)
                     /gene="fumC"
                     /locus_tag="POB34_001331"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDI5918316.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTALGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(16318..17964)
                     /gene="fumB"
                     /locus_tag="POB34_001332"
     CDS             complement(16318..17964)
                     /gene="fumB"
                     /locus_tag="POB34_001332"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDI5918317.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            18163..19338
                     /gene="manA"
                     /locus_tag="POB34_001333"
     CDS             18163..19338
                     /gene="manA"
                     /locus_tag="POB34_001333"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HDI5918318.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFSIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCQGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            19439..20947
                     /locus_tag="POB34_001334"
     CDS             19439..20947
                     /locus_tag="POB34_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDI5918319.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(20992..22257)
                     /gene="uidC"
                     /locus_tag="POB34_001335"
     CDS             complement(20992..22257)
                     /gene="uidC"
                     /locus_tag="POB34_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HDI5918320.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFRFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSALGDRLNYWDVKMSIQYDFMLK"
     gene            complement(22296..23668)
                     /gene="uidB"
                     /locus_tag="POB34_001336"
                     /pseudo
     CDS             complement(22296..23668)
                     /gene="uidB"
                     /locus_tag="POB34_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            complement(23665..25476)
                     /gene="uidA"
                     /locus_tag="POB34_001337"
     CDS             complement(23665..25476)
                     /gene="uidA"
                     /locus_tag="POB34_001337"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDI5918321.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAANKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(25865..26455)
                     /gene="uidR"
                     /locus_tag="POB34_001338"
     CDS             complement(25865..26455)
                     /gene="uidR"
                     /locus_tag="POB34_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HDI5918322.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(26684..27451)
                     /gene="hdhA"
                     /locus_tag="POB34_001339"
     CDS             complement(26684..27451)
                     /gene="hdhA"
                     /locus_tag="POB34_001339"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HDI5918323.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(27563..28591)
                     /gene="malI"
                     /locus_tag="POB34_001340"
     CDS             complement(27563..28591)
                     /gene="malI"
                     /locus_tag="POB34_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HDI5918324.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            28766..30358
                     /gene="malX"
                     /locus_tag="POB34_001341"
     CDS             28766..30358
                     /gene="malX"
                     /locus_tag="POB34_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HDI5918325.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLNC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            30368..31540
                     /gene="malY"
                     /locus_tag="POB34_001342"
     CDS             30368..31540
                     /gene="malY"
                     /locus_tag="POB34_001342"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HDI5918326.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRIYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            31644..32645
                     /gene="add"
                     /locus_tag="POB34_001343"
     CDS             31644..32645
                     /gene="add"
                     /locus_tag="POB34_001343"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDI5918327.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLAKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(32680..33720)
                     /locus_tag="POB34_001344"
     CDS             complement(32680..33720)
                     /locus_tag="POB34_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDI5918328.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            33963..34088
                     /gene="blr"
                     /locus_tag="POB34_001345"
     CDS             33963..34088
                     /gene="blr"
                     /locus_tag="POB34_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HDI5918329.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            34361..34576
                     /gene="ydgT"
                     /locus_tag="POB34_001346"
     CDS             34361..34576
                     /gene="ydgT"
                     /locus_tag="POB34_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HDI5918330.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            34662..35102
                     /locus_tag="POB34_001347"
     CDS             34662..35102
                     /locus_tag="POB34_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HDI5918331.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            35179..35760
                     /gene="rsxA"
                     /locus_tag="POB34_001348"
     CDS             35179..35760
                     /gene="rsxA"
                     /locus_tag="POB34_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDI5918332.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            35760..36338
                     /gene="rsxB"
                     /locus_tag="POB34_001349"
     CDS             35760..36338
                     /gene="rsxB"
                     /locus_tag="POB34_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HDI5918333.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            36331..38457
                     /gene="rsxC"
                     /locus_tag="POB34_001350"
     CDS             36331..38457
                     /gene="rsxC"
                     /locus_tag="POB34_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDI5918334.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQSNAEPEEQVDPRKAAVK
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQTNAEPEEQVDP
                     RKAAVAAAIARAQAKKAAQQKVVNED"
     gene            38458..>39007
                     /locus_tag="POB34_001351"
     CDS             38458..>39007
                     /locus_tag="POB34_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HDI5918335.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGI"
BASE COUNT         9521 a        10082 c         9571 g         9833 t
ORIGIN      
        1 gattaccgtt attgttcgca gaaggctttt tgctgatggg gtcgttcgtc acgctgttta
       61 attacatcgg ctatcggttg atgctttccc cctggcatgt cagtcaggcc gtggttggct
      121 tattatcgct ggcttatttg accggtacat ggagctcacc taaagccgga accatgacca
      181 cccgctacgg gcgcggtccg gtgatgttgt tttcgacggg ggttatgctg tttggtttac
      241 tgatgacctt attcagctcg ctgtggctga tctttgccgg aatgttactc ttctcagcag
      301 gattcttcgc agcccactca gttgccagca gctggattgg cccccgcgca aaacgcgcta
      361 aaggccaggc ctcctcgctg tatctgttca gttactatct ggggtcgagt attgccggga
      421 cgctgggtgg tgttttctgg cataactatg gctggaacgg cgtcggcgca tttattgctc
      481 tgatgctggt cattgctctg ctggtcggga cgcgtttgca tcgtcgtctg cacgcctgaa
      541 aaataagccc ggactgcggt aactacacgt ccggacttat tgcttgctca aaccaacgtt
      601 aatagccatc ctaaaataga cgaagcgcca gccaattccc gcagcgcgtc tagcgtcatc
      661 aggattataa gtacccaaat aaacggattc attttgctgt gtgtcattta ttactgatgc
      721 gcagttattc taccgctttg taagtagtaa aatagttaac ccgatcaaga ctactattat
      781 tggtagctaa atttccctta agtcacaata cgttattatc aacgctgtaa tttattcagc
      841 gtttgtacat atcgttacac gctgaaacca accactcacg gaagtctgcc atttccaggg
      901 atatagttat ttcaacggcc ccgcagtggg gttaaatgaa aaaacaaatt gagggtatga
      961 caatgaaaaa agtattagct ctggttgttg ccgctgctat gggtctgtct tctgccgcct
     1021 ttgctgcaga gactgcgacc acacctgctc cgactgcgac gaccaccaaa gcagcgccgg
     1081 cgaaaacgac acatcataaa aaacagcata aagcagcacc tgcccagaaa gcgcaggcgg
     1141 ctaaaaagca tcataaaaat acgaaagctg aacagaaagc ccctgaacaa aaagcgcagg
     1201 cagcgaagaa acacgccggg aaacacagcc atcagcaacc ggcaaaacct gctgcacaac
     1261 ccgcagcgta agttttcaac agtaatgctg gcgcgccccc tcgcgcctga aaattacggt
     1321 gctaagcggg taacgtttag caccgccttt agcggagggt agtatggtgg gccgttatcg
     1381 ctttgagttc attcttatca tccttatttt atgcgcactg attgccgccc gtttttatct
     1441 ttcctgactg tagttatctg attttactcc cactttcatc ccgtcccgtc tatagtattt
     1501 atgagggttt gcttttaata atcataatta cccaccagag tgtgatatgc gtacaaccat
     1561 tgctgtagtg ttgggtgcaa ttagtttgac gtctgctttt gtgtttgcag ataaaccaga
     1621 cgttgccaaa tcggcaaacg atgaggtcag caccctgttt tttggtcatg atgatcgtgt
     1681 gccagtgaat gacacgaccc aatcaccgtg ggatgcggtt gggcaactgg aaacggccag
     1741 cggcaattta tgtacggcga cgctgattgc acccaatctg gcattaacgg caggacactg
     1801 tttattgacg ccgcctaagg gtaaagcgga taaggcggta gcgctgcgtt ttgtgtcaaa
     1861 taaaggtctt tggcgctatg acatccacga catagaaggc cgcgttgatc cgacactggg
     1921 aaagcggtta aaagcagatg gggatggttg gattgttcct cccgcagctg caccatggga
     1981 cttcggcttg attgtgctgc gtaatccccc ttctggcatt acaccgttgc cgttatttga
     2041 gggagataaa gccgcgctta ctgccgcatt aaaatcggct ggtcgtaaag tgactcaggc
     2101 gggatatcct gaagatcatc tcgatacgtt atacagtcat caaaactgtg aagtgactgg
     2161 ctgggcgcaa acgtcggtga tgtcacatca gtgcgatacc ttgccgggtg acagcggttc
     2221 gcctctgatg ttgcataccg atgacggctg gcaattaatt ggggtgcaaa gttcggctcc
     2281 tgccgcgaaa gatcgctggc gcgccgataa ccgggccatt tctgttaccg gttttcgcga
     2341 caagctggat caactgtcgc aaaaataatg ttcaaacgct gcccgacagt gcgggcagcg
     2401 tgttcatcag gcaagtttca ccatgatcat tccagccaac agcaagacca ggccaatcca
     2461 gcctttacga tttaaccgtt gaccaaacaa gatccaacct gcggctaacg tggcggcaat
     2521 accaaacccg ccccacaatg cataagcgac agacaagtcg atccctttta cggcctgaga
     2581 aagcgcacta aaggcagcca gcaccgccgc cagggagagc aagccaaata ttttgcgacg
     2641 aaagccgtca gaaaatttca aaaagacgtt agcaacgatt tccagcacga ttgccaatgc
     2701 cagccaggcg gcgtgaaccc attcaaactg cgccatggtt cacctccagt tcaggtttac
     2761 gcgctttacg ggtacctgat ttgatcaaca caatcccggc gaccagggtg gttaacccga
     2821 caattttcat cagcgataaa ctttcgtcga ataacaaaac gctaaacaag gtaataaata
     2881 aaataccgat accttcccac agcgcataag ctacgcctaa ggcgatcttt ttaacggcga
     2941 aagagagaaa tatatacgac agagaaatca tcaccagcat taaaataaag ccgccatttc
     3001 cctcactgac gctcgcccat ttcattgaca gcgtaccggt aatttctgta gcaatagcca
     3061 ggcctaataa aatccaataa atatacattg tccttctcct gcaagagaat tattttaatt
     3121 ttcgcttaat tcagcgaaac caaagtaaag tggtcaagcc ccgcgcatag ggcaagctta
     3181 ggcagaagaa aggactaaag cgcgttgcgc cagtgctgct cacctacgag caagatagtg
     3241 gatgaggtac gaagatgggt aaatgttgaa aataacgtcc tgaacaaatt gtccataata
     3301 ttacaattat ccgcagtgtt gcttctcgtc atcgcggatg ataattgtcc tcggtagttg
     3361 aacacgcctg atttgtatca tagcttaaga attaactcaa aatattttca cttctttacc
     3421 tgagcggttt gattttcgtt atgatgacgg agcgaaaaag acattattat tagcaaagga
     3481 agaaaaaacg gggacaagca tggcaaagcc gatcatcacg ctcaatggcc taaaaatcgt
     3541 cattatgttg ggaatgctgg tcattattct ctgcggtatc cgttttgccg ccgagatcat
     3601 cgtgccgttt attctcgcat tatttattgc tgttattctt aacccgctgg tgcaacacat
     3661 ggtccgctgg cgagtgccgc gtgtactggc ggtgtcgatt ttgatgacca tcatcgtgat
     3721 ggcgatggtg ttgctgttag cttatctggg ttccgcgctc aacgagttga cgcggacgtt
     3781 accgcaatat cgcaactcta ttatgacgcc gctgcaagct cttgagccgt tgttgcaacg
     3841 cgtagggatt gacgtctcag ttgaccaact ggcgcattac attgatccga acgcggcgat
     3901 gacgttgctc accaacttat tgacgcagtt atctaatgcc atgtcatcaa tatttttatt
     3961 gctgctgacg gtgctgttta tgctgctcga agtgccacaa ttgcccggaa aatttcagca
     4021 aatgatggcg cgtccggttg aagggatggc ggcgattcaa cgtgcgattg acagtgtttc
     4081 tcattatctg gtgctgaaaa cagccatcag catcatcacc ggcctggtcg cctgggcgat
     4141 gctcgccgca ctcgatgttc gcttcgcttt tgtctgggga ttgctggcct ttgcgcttaa
     4201 ttacatccct aatattggtt cagttctcgc ggcaatcccc cctatcgctc aggtactggt
     4261 gtttaatggc ttctacgaag cgttgctggt gctggcggga tatctgctga ttaatctggt
     4321 cttcggcaat attctggagc cgcgcatcat gggacgtggg ctggggcttt cgacattggt
     4381 ggtatttttg tcgttgattt tttggggatg gttgttagga ccggtgggta tgctgctttc
     4441 cgtgccgttg acaattattg tcaaaattgc gcttgaacaa acagcgggag gtcaaagcat
     4501 cgccgttctg ttaagcgatc tcaataaaga gtgacggcct ccgcagaggc cgtcagggtt
     4561 acagagcttt caggattgca tccacgctgg ctttggcgtc accaaacagc atgtgggtgt
     4621 tttccttgaa gaacagcggg ttttgcacac cagcatagcc agtgttcatc gaacgtttaa
     4681 agacaatcac gttctgcgct ttccacactt ccagcacagg cataccagca ataggactct
     4741 tcggatcatc ctgcgccgcc gggttaaccg tatcgttagc accaatcacc agtacggtat
     4801 cggtatcagc aaagtcatcg ttgatctcgt ccatttccag cacgatgtca tacggtactt
     4861 ttgcttcagc cagcaatacg ttcatatgtc caggcaaacg ccccgcaacc gggtggatac
     4921 cgaaacgcac attaatacca cgagcgcgca atttctcagt aatttcagcg acaggatatt
     4981 gcgcctgcgc aactgccatg ccgtaccccg gagtaatgat cactgaatgg gagtttttca
     5041 gcagttccgc tgtctcttct gcagtgattt cgcggtgctc gcccacttcc tgatcatcac
     5101 cagtagaaga gccgtcggtg ccgaaaccac ccgcaataac gctgataaag gaacggttca
     5161 tcgccttaca cataatgtaa gaaaggatag cccccgaaga accgaccagc gcaccggtca
     5221 caatcagcag gtcgttgctg agcataaagc ccgcagccgc agccgcccag ccggagtacg
     5281 agttcagcat cgacaccacc actggcatat ctgcaccacc gatggaggcg actaaatgcc
     5341 agccgaatac cagcgcaatg gcggtcatta tcagcaatgc cagcacttgc aggccgacgc
     5401 tatccgtgcg aacaaataca atcagcagca ggaaggaaac gaccagagcc gccaggttca
     5461 ttttgtgacg gtttggcagc atcaacggtt tagacgaaat cttgccacac agtttgccga
     5521 acgccaccac cgaacccgtg aacgttaccg ccccgatgaa gataccgagg aacacttccg
     5581 tcaggtgaat attgaccaga atcggtgcca ttcccgcgtc atgatgcaga tagctgttaa
     5641 agccaaccag cactgccgcc agacccacga agctatgcag gatcgccacc agttctggca
     5701 tttcggtcat ttcaactttc ttcgccagac ggataccaat tgccccacca atgaccatcg
     5761 ccagcaagat ccagccaaca ttacccgtat ccggtccaaa aatggttgcg attaacgcaa
     5821 tcgccatccc ggcgataccg aagttgttac cctggcgaga cgtttcatgt ttcgaaagac
     5881 cggccagact gaagataaac aggatcgcgg caacaatgta tgcagctgta actaatcctc
     5941 cagacatatg ttacccctta atttttgcgg aacattttca gcatgcgctg agtcacggtg
     6001 aagccaccga aaatattaat gctggctata agcaccgcga taaaactaag gaagctaacc
     6061 cagccgccct ggccaatctg caacagtgct ccgacaacaa taatccctga aatcgcgttg
     6121 gtgaccgaca tcaacggtgt atgcagcgca tgcgatacat tccacaccac gtaataaccg
     6181 acaacgcagg ccagcgcgaa aacggtgaag tgcccaagaa actctttcgg cgcaacgctt
     6241 gccagccaac cgaaaaggat gattgccagc gccatcaatg cgtatttacg ccacggtgag
     6301 caggcacatt tttcctcagt tttcacttcc ggtgccgctt tttgtgccgc ctgcggctga
     6361 gctgatacct gaatcggcgg tgccggccag gtaatttcgc ccgcacggat cacggtcacg
     6421 ccgcgaatca ccacatcatc aaaatcaaca gtgatattgc cgtctttctc tttgcacaac
     6481 agtttcagca gattaacgag gttagtaccg taaagctgtg aggattgcgt cggcagacgg
     6541 cccggaagat cggtataacc aatcactttg acaccatttt ccgtagtgaa gatttcaccc
     6601 ggcacggtgt atgcacagtt gccgccgttt tgggctgcca ggtcgacaat cacactgccc
     6661 gccttcatgg agtcaaccat ttcacgggta attagcttcg gcgctggttt gcctggaata
     6721 agcgcggtgg tgacaatgat atcgacctct tttgcctggg cggcaaagag ttccatttcc
     6781 gctttgatga acgcgtccga catcactttg gcatagccat cgccgctgcc cgcttcctct
     6841 ttaaaatcca gctcgaggaa ttccgcgccc atactttgaa cttgttcttt cacttccggg
     6901 cgggtgtcga atgcacgcac aatcgcgccg agactgtttg ctgcgccaat ggcggccaga
     6961 cctgcaacac ccgcaccaat caccatcact tttgccggtg gcactttccc ggccgcagta
     7021 atttgcccgg taaagaagcg cccaaattca tgtgccgctt caacaatggc gcgataaccg
     7081 gcgatgttcg ccatcgagct tagtgcgtcc agcgattgtg cgcgtgagat acgcggcaca
     7141 gagtccatcg ccatcacggt cacgttacgt tccgcaagtt tttgcattaa ttccggattc
     7201 tgcgcaggcc agataaaact caccagcgtt gtcccaggat tcagtaacgc aatttcatca
     7261 tctaacggcg cattgacctt cagaatgatc tctgactgcc agacgctatt cccttctaca
     7321 atttcagcgc ccgcttgcac aaacgcttta tcgtcaaaac ttgccagttg acccgcgccg
     7381 ctctctaccg cgacggtaaa acccagtttc agcagctgtt ccactgtttt tggcgttgct
     7441 gcaacacggg tttcattggt taaccgttct cttggtatgc caattcgcat gatattccct
     7501 tccatcggtt ttattgatga tggtttgcct gtgtcaggag ccacacaagc cgctcatgta
     7561 cgagctaaat gttactccgt taaaataaat tagtaacaaa cgccctataa cgtactgaaa
     7621 attatgcctg tgatctagcg ccaaaaatca gtatttcggc gtgaactcgc aaaatattaa
     7681 cgattcagcc gtgatagtgg gataaacacc ttagaacgcc ggataaagac tgataattgt
     7741 cttcgacggt cgggtaaaac gagacatcgc cccggcacgc atcactactt aacattaaat
     7801 taacttatac aattcagttg cttcagtagt aatgatgctg atacggctgt tttttaagca
     7861 tagacggtca tttgagcagg attaaaattg gcttaaggaa tgtgatatga aaaatgacgc
     7921 agacagttac accgtttaaa tgcaataatc agccacgttt ctcgttaata acaataccag
     7981 tacttggttt gcgcaaggcg aaggattatt tttatgaagc ttaagaacac cctcctggcg
     8041 tcggcactgc tttctgctac ggcattctcc gttaacgcag caacagaact gacaccggag
     8101 caagcggcag cggttaaacc ttttgaccgt gtagtggtta ccggtcgttt taatgctatt
     8161 ggcgaagcgg tgaaagccgt ttctcgtcgc gcagataaag aaggtgccgc ctctttttat
     8221 gttgtcgaca cttctgattt tggtaacagc ggtaactggc gtgtggtcgc tgacctctat
     8281 aaagccgatg ctgaaaaagc agaagaaaca agtaatcgcg taattaacgg tgttgtcgaa
     8341 ctgccgaaag atcaggctgt tctgattgaa ccgtttgaca cggtcaccgt ccagggcttc
     8401 tatcgtagcc agccagaagt caatgatgcc atcaccaaag cagcaaaagc gaaaggtgcc
     8461 tactctttct acatcgttcg tcaaatcgat gccaaccagg gcggcaacca gcgtattact
     8521 gcattcatct ataaaaaaga tgctaagaaa cgtatcgtcc agagcccgga tgtgatcccg
     8581 gcagattccg aagcaggacg tgcagctctg gctgccggtg gcgaagccgc gaagaaagtt
     8641 gagatcccgg gtgttgcgac taccgcatca ccaagttctg aagtcggtcg cttctttgaa
     8701 acccagtcat caaaaggcgg gcgttacacc gtcacgctcc cggatggcac taaagtcgaa
     8761 gaactgaaca aagcgaccgc agcgatgatg gtcccgttcg acagcatcaa attctctggc
     8821 aactacggca acatgaccga agtctcctat caggttgcga aacgtgccgc gaagaaaggt
     8881 gctaagtatt accacatcac ccgccagtgg caggaacgtg gtaataacct gaccgtcagc
     8941 gcagatctgt ataaataact attcagttac tcctataagg cggcttgatg ccgcctttcg
     9001 catttttagt cacaaatttc cccgcgacca ttgcatgcag acacatctct tcgtaaaatc
     9061 ccgcgcttca gtggcaaact ccatttttat gcgttttgcc ctaatattta ttcttcttat
     9121 cacgttttaa tcactggata tcgatggaaa agaaactggg actgagcgca ctcaccgcgc
     9181 tggtattaag ctcaatgctg ggcgcgggtg ttttcagtct gccgcaaaat atggcggcag
     9241 ttgccagccc ggcagcactg ctcatcggct ggggtattac tggcgctggc attttattgc
     9301 tggcctttgc catgctgatc ctcacgcgca ttcgtcccga acttgacggc ggtatcttta
     9361 cctatgcccg cgaagggttt ggcgagctaa tcggcttttg ttccgcatgg ggatactggc
     9421 tgtgcgcagt catcgccaac gtctcctatc tggtgatcgt tttttccgcg ttaagctttt
     9481 ttacggacac gccggaattg cgcctgttcg gcgatggtaa tacctggcaa tcgatagtcg
     9541 gtgcatcggc tttattgtgg attgttcact ttttgatcct gcgcggggtg caaaccgctg
     9601 ccagcattaa cctggtggcg acactggcaa aattgttgcc gctgggtctg tttgttgtgc
     9661 tggcgatgat gatgttcaaa ctggatacct tcaagctcga cttcaccgga cttgcacttg
     9721 gcgtacccgt gtgggaacag gtgaaaaaca ccatgctgat caccctgtgg gttttcattg
     9781 gtgtggaagg tgccgtggtt gtttctgcgc gtgcgcgtaa taaacgtgat gttggcaaag
     9841 cgacactgct ggcggttctc tccgctctgg gcgtttactt gctggtaacg ctgctttcgc
     9901 tgggcgtggt ggcacgtcct gaactggctg aaattcgtaa cccgtcaatg gccggtctga
     9961 tggtggaaat gatgggacca tggggcgaaa tcatcatcgc tgccggtttg atcgtttccg
    10021 tttgcggcgc gtacttaagc tggaccatca tggcggcaga agttccgttc ctggctgcca
    10081 ctcataaagc attcccacgc atttttgcgc gccagaacgc acaagctgcg ccatccgcct
    10141 cgctgtggtt gaccaacatc tgcgtgcaaa tttgtctggt actcatctgg ctgaccggtt
    10201 ccgattacaa cacgttgctg accatcgcct cagaaatgat tctggtgccc tatttcctgg
    10261 tcggcgcgtt cttgctgaaa atcgcaaccc gtccactgca taaagcggta ggtgtcggtg
    10321 cctgcattta tggcttatgg ttattgtatg cctctggtcc aatgcacttg ttgttatccg
    10381 ttgttctgta tgcgccgggg ctgctggttt tcctgtatgc gcgtaaaacg catacacatg
    10441 ataacgtact gaaccgccag gaaatggtgc taattggcat gctgttaatt gcctcagttc
    10501 cggcgacctg gatgctggtg ggataacgtt catcccatcg ttggttagca aaggagataa
    10561 cgatgggtaa agcccagccc ttgccaatat taattactgg cggtggtcgt cgcatcggcc
    10621 tcgccctcgc atggcatttc attaatcaaa agcaaccggt gattgtcagc tatcggacac
    10681 actatccagc cattgatgga ctgattaatg caggtgcgca gtgtattcag gctgattttt
    10741 cgaccaacga cggtgtgatg gcgtttgccg atgaagtact aaaaagcacc catggtctgc
    10801 gtgctatttt gcataacgcc agtgcgtgga tggcggaaaa accgggtgcg ccactggccg
    10861 acgtactggc ttgcatgatg cagatccacg ttaatacccc atacctgctc aaccatgcgc
    10921 tggaaagatt actgcgtggg cacggacacg ccgccagcga tatcattcac tttaccgatt
    10981 atgtggtgga gcgcggtagc gacaaacatg ttgcgtatgc tgcaagcaaa gcggcactgg
    11041 ataatatgac ccgctcgttt gcccgcaagc tggcaccgga agtgaaagtg aattctattg
    11101 cgccatcgct gatcctgttt aatgaacatg atgatgccga atatcgacaa caggcgctga
    11161 ataaatcact gatgaaaacc gcgcctggcg agaaagaagt gatcgacctg gtcgattact
    11221 tacttaccag ttgctttgtc accggacgca gtttcccact tgatggcggt cgtcatctgc
    11281 gttaatgcag ctttatccag catataatca acacccatgc gcttatcccc caacacgcaa
    11341 cagaaccaac aaaagcggcg ggcaagcgca tcattccggt gaaataccac agcgacacca
    11401 ggtaaacaaa atagggaata atcgaccaca tactaaaaat gatggttgcg cgtaaggctt
    11461 caatgccgcg ttcgctggca acaatataat gcgcgataag cgcaaaggtc gggaaaagtg
    11521 gaatcagccc ggcgatataa taatttttcg tttttgctaa aacaccaatc aacagcacta
    11581 ccagcgcacc gagcgcggct ttgattacca gccccatttt tttaccttaa cacttccata
    11641 acaagtcatc agtagaatac ctgatgaaaa cttgtttaga aacgattgat agtaagtaaa
    11701 aacagcgcgg tgtattgtga cgtttttata tctaccgtga ataatatgaa cactatcgta
    11761 tttgtggaag atgatgcgga agtcggttca ctgattgccg cgtacctggc aaaacatgat
    11821 atgcaggtta ccgtagagcc gcgcggcgac caggccgaag aaaccatttt gcgagaaaat
    11881 ccggatttgg tgttactcga catcatgcta ccaggcaagg acggcatgac catttgtcgt
    11941 gatttacgcg caaagtggtc tggaccgatt gttcttctaa cctctctcga tagcgatatg
    12001 aaccacatcc tggcactgga aatgggtgcc tgcgactata ttctcaaaac gacgccccct
    12061 gctgttttgc tagcgcgttt acgtttgcat ttgcgtcaga atgagcaagc cacactgacc
    12121 aaaggtcttc aggaaacgtc tctgactccc tacaaagccc tgcatttcgg cacgttgacc
    12181 atcgatccca tcaaccgcgt agtcaccctg gctaacactg aaatctcgct ctcgacagct
    12241 gatttcgaat tattgtggga attagctacc catgccgggc aaatcatgga ccgcgatgca
    12301 ttgctgaaaa atttacgcgg cgtcagttat gacggactgg atcgtagcgt ggacgtggct
    12361 atttcgcggt taagaaaaaa actgctcgat aacgccgcag aaccttatcg cattaaaact
    12421 gtgcgtaaca aaggctatct ttttgcgcct catgcatggg aataagcgat gaaaaaactg
    12481 tttatccagt tttacctgtt attgtttgtc tgcttccttg tgatgtctct gctggttggg
    12541 ctggtgtaca aatttaccgc cgaacgcgcg ggcaaacagt cgctggatga tttgatgaac
    12601 agttcgctgt atctgatgcg tagcgaattg cgtgagatcc ccccacacga ttggggtaag
    12661 acgctgaagg agatggattt aaatctctct ttcgatctgc gtgtcgagcc actgagtaaa
    12721 taccatcttg atgatatttc catgcaccga ctgcgtggcg gcgaaattgt cgccctggac
    12781 gatcagtaca cgtttttgca gcgtatcccg cgcagccact acgtgctggc agttggtcct
    12841 gttccttatc tttattatct ccatcagatg cgattgctgg atatcgccct gatcgctttt
    12901 attgctattt ccctcgcctt tccggtgttt atctggatgc gtccgcactg gcaggatatg
    12961 ttaaaactgg aagcagcggc gcaacgattt ggcgatgggc atctcaatga gcgtatccac
    13021 tttgatgagg gttcgagctt tgaacgactt ggtatcgcat ttaaccagat ggcggacaat
    13081 atcaacgcct tgattgccag caaaaaacag cttattgacg gtatcgctca cgaactgcga
    13141 acaccgttag tgcgcctgcg ttatcgactg gagatgagcg ataacctgag cgccgccgaa
    13201 tcccaggcgt tgaatcgtga tatcagtcaa cttgaagctt taattgaaga gctgctgact
    13261 tatgcccgac tcgatcgccc acaaaacgag cttcatctta gcgaaccaga cctgccgttg
    13321 tggctgtcaa cgcatctggc agatattcag gcagtaacgc ccgataaaac ggtacggata
    13381 aaaacgctcg tgcaaggcca ttatgcggcg ttggatatgc gcttaatgga gcgcgtgctg
    13441 gataatttgc tcaataacgc cctgcggtac tgccattcaa cggttgaaac cagcctgcta
    13501 ctgtcgggga atagagcgac attaattgtt gaggatgatg gcccagggat tgccccagaa
    13561 aaccgcgaac atatctttga accttttgtt cgcctcgatc ccagccggga tcgctcaacc
    13621 ggcggctgcg ggctggggct ggcaattgtc cactctatag cactggcaat gggcggtacg
    13681 gttaattgtg acaccagcga actgggtggt gctcgcttct cgtttagctg gccgttatgg
    13741 cataacatcc cgcaatttac ctctgcctga cactacgcgc acgatggtca agtcaccacc
    13801 attgtgctat aaattaagga tgttgtaact aaagtggtta atattatggc gcgttacgat
    13861 ctcgtagacc gactcaacac tacctttcgc cagatggaac aagagctggc tgcatttgcc
    13921 gctcatcttg agcaacacaa gctattggtt gcccgcgtgt tctctttgcc ggaggtaaaa
    13981 aaagaggatg agcataatcc gcttaatcgt attgaggtaa aacaacatct cggcaacgac
    14041 gcgcagtcgc tggcgttgcg tcatttccgc catttattta ttcaacaaca gtccgaaaat
    14101 cgcagcagca aggccgctgt ccgtctgcct ggcgtgttgt gttaccaggt cgataacctt
    14161 tcgcaagcag cgttggtcag tcatattcag cacatcaata aactcaagac cacgttcgag
    14221 catatcgtca cggttgaatc agaactcccc accgcggcac gttttgaatg ggtgcatcgt
    14281 catttgccgg ggctgatcac ccttaatgct taccgcacgc tcaccgttct gcacgacccc
    14341 gccactttac gctttggttg ggctaataaa catatcatta agaatttgca tcgtgatgaa
    14401 gtcctggcac agctggaaaa aagcctgaaa tcaccacgca gtgtcgcacc gtggacgcgc
    14461 gaggagtggc aaagaaaact ggagcgagag tatcaggata tcgctgccct gccacagaac
    14521 gcgaagttaa aaatcaaacg tccggtgaag gtgcagccga ttgcccgcgt ctggtacaaa
    14581 ggagatcaaa aacaagtgca acacgcctgc cctacaccgc tgattgcact gattaatcgg
    14641 gataatggtg cgggcgtgcc agacgttggt gagttgttaa attacgatgc cgacaatgtg
    14701 cagcaccgtt ataaaccaca ggcgcagccg cttcgtttga tcattccacg gctgcacctg
    14761 tatgttgcag attaacgccc ggctttcata ctgccgacca tctgttctgg ccgtacccag
    14821 ctgtcaaact cggcttcgct aagatacccc agcgcaaggg ccgcagcttt taaggtcagc
    14881 ccttctttat gcgctttttt ggcgatctcc gcggctttgt cataaccaat gtgggtgtta
    14941 agcgcagtca ccagcatcag cgattcattg agtaattgat tgattcgctc acgattcggt
    15001 tcaataccca ctgcgcagtg tttgttaaaa ctttccatgc catctgccag caagcgcacc
    15061 gattgcagga aattatggat caccatcgga cggaagacgt tcagttcaaa gttaccggaa
    15121 gcgccaccca tgttgatcgc cacgtcgttc cccattacct gacagcagag catggttaat
    15181 gcttcacact gggttgggtt cactttcccc ggcatgattg agctgcccgg ctcattttcc
    15241 gggattgaga tttcaccaat tccgcagcgc gggccagagg ccagccagcg gacatcattg
    15301 gcgattttca tcagtgacgc agccaaccct ttcaacgcgc cgtgcgcctg aaccagggca
    15361 tcacaggtcg caagcgcttc aaatttgttc ggcgcggtaa caaacggtgc acaggtaatg
    15421 actgccagtt catctgctac gcgacgcgca tactccggat gggtatttag tccagtaccc
    15481 aacgctgtac cgccaagagc cagttccgct acgtgaggca ggctgtattc gatatgtttg
    15541 agattatgct cgagcatcgc tacccagccg gaaatctcct gccctagtgt tagcggcgtg
    15601 gcgtcctgca agtgggttcg accgattttg acgatatcgg caaatgcacg cgatttttca
    15661 ctcagtgtct gtgtcagggt tttaagctgc ggaatgagtt gcttgcgcag cgccagcagc
    15721 gccgcaacgt gcatcgccgt cggaaagaca tcgttggaac tttggctttt gttcacgtcg
    15781 tcgttagggt gaactttacg ttccatcccg cgcacgccgc cgagtaattc actggcccgg
    15841 ttagccagca cttcgttcat gttcatgtta ctttgcgtgc cggagccggt ctgccagata
    15901 gccagcggga attcgtcgtc atgctgtcct gccagtactt catccgccgc ctgccgaatg
    15961 gcgctcgctt tctcttcaga caacaagcct aaatcttcat taacttttgc cgctgcgcgc
    16021 ttggttagcg ccagcgcatg aatcagtgag gtgggcattt tctccgtcga aatgcggaaa
    16081 tgctccagcg agcgttgagt ttgtgcgccc cacagcttat ctgccgggac atcaatcgcc
    16141 cccatcgaat ctttttcgct gcgtactgta ttcatggcct gctcctcacc tgtttaattt
    16201 tttctttctg ttttgctttc gttaagcaac ttttagctca ctcattattt actatttgat
    16261 aacaaatgtt tggtctttcg tgccatgtaa aaaaaccgcc ccgaagggcg gctctgttta
    16321 tttcacacag cgggtgcatt gtgtgagttg tatctgctgg aagaagtcat ttcctttatc
    16381 atccacaagg ataaacgccg ggaaatcttc cacttcaatt ttccagatgg cttccattcc
    16441 cagttccgga tattcaacac attccaggct cttaatactt ccctgcgcca atacagcggc
    16501 cggaccaccg atactgccga ggtagaagcc gccgtgtttt ttacaggcat ccgtcacctg
    16561 ctggctgcgg ttgcctttcg ccagcatgat catacttccg ccctgcgctt gcagttgatc
    16621 gacataagaa tccatccgtc cggcggtcgt tgggccaaga gaaccggaag cataaccttc
    16681 cggcgttttg gccggacccg cgtagtaaat cggatgatct ttaatgtact gcggcagccc
    16741 ttcaccatta tccatccgct ctttcagttt ggcgtgagca atatcacgac cgacgataat
    16801 cgtgccgtta agcgataagc gtgtagaaac gggatactgc gacaactgtg cgaggatctc
    16861 tttcatcgga cggttaaggt caacgcgcac cgcttcgcct tctcccgctt tgcgcagctc
    16921 ttccgggata tatttgcctg gattatgttc cagtttttcg atccagatcc cctgacggtt
    16981 gatcttcgct ttgatattac ggtcagcaga gcaggagacg cccataccga ccgggcagga
    17041 tgcgccgtga cgtggcaggc gaatcacgcg gatgtcgtga gcgaagtatt taccaccaaa
    17101 ctgcgcaccc agaccgagat tttgcgcttc gatcagcaat tctttttcca gttccacatc
    17161 gcggaatgcc tgaccgtgct cattcccttc cgttggcagt tcgtcatagt atttagcgga
    17221 agccagtttc actgttttaa ggttcgtttc tgcagaagtt ccaccaataa cgaacgcaat
    17281 atgatacgga ggacaggccg ccgtacccag cgtgcgcatc ttctcaacca ggtaattttt
    17341 cagtttcccc ggcgttagta acgctttggt ttcctgatag agatacgtct tgtttgccga
    17401 accgccacct ttggcgatac agaggaattt gtactcgtcg ccatcaacgg cataaagatc
    17461 gatctgcgct ggtagattgg tgccggtatt cacctcttta tacatatcca gcggcgcgtt
    17521 ttgcgagtag cgcagattat cttcgatata agtgttatag acaccgcgtg ccagcgccgc
    17581 ttcatcaccg ccaccggtcc atacacgctg ccctttttta ccaacaataa tcgcggtgcc
    17641 ggtatcctga caggttggca gaacgccttt cgccgcgatg tcggagttgc gcaggaattg
    17701 cagcgccaca tatttatcat tttcgctggc ttccgggtca cgcagaatgt cggccacctg
    17761 ttgttggtga gccggacgca gcataaatga cgcatcatga aacgcctgac gcgccaacag
    17821 agttaacgct tcgggtgcga ctttcaaaat ctcctgccct tcaaattcag atacgctaac
    17881 gtgttcgctg gttagcaggt aatactcagt atcatctttt ttgagtggga aaggagcctg
    17941 ataatgaaag ggtttgtttg acattattct ctcacttact gcctggtttg gttatgctct
    18001 gggcgggtgt tccgttgccc tgttaaaagc gagtaacaat atcctacaca cttttttaac
    18061 aaaaactgag actagtacga ctttttgcgg ctccaggtta cttcccgtag gattcttgct
    18121 ttaatagcgg gattaatttc cacattaaaa cagggattga tcatgcaaaa actcattaac
    18181 tcagtgcaaa actatgcctg gggcagcaaa acggcgttga ctgaacttta tggtatggaa
    18241 aatccgtcca gccagccgat ggccgagctg tggatgggcg cacatccgaa aagcagttca
    18301 cgagtgcaga atgccgccgg agatatcgtt tcactgcgtg atgtgattga gagtgataaa
    18361 tcgactctgc tcggagaggc cgttgccaaa cgctttggcg aactgccttt cctgttcaaa
    18421 gtattatgcg cagcacagcc gctctccatt caggttcatc caaacaaaca caattctgaa
    18481 atcggttttg ccaaagaaaa tgccgcaggt atcccgatgg atgccgccga gcgtaactat
    18541 aaagatccta accacaagcc ggagctggtt tttgcgctga cgcctttcct tgcgatgaac
    18601 gcgtttcgtg aattttccga gattgtctcc ctactccagc cggtcgcagg tgcacatccg
    18661 gcgattgctc actttttaca acagccaaat gctgaacgtt taagcgaact gttcgccagc
    18721 ctgttgaata tgcagggtga agaaaaatcc cgcgcgctgg cgattttaaa atcggccctc
    18781 gatagccagc agggtgaacc gtggcaaacg attcgtttaa tttctgaatt ttacccggaa
    18841 gacagcggtc tgttctcccc gctattgctg aatgtggtga aattgaatcc tggcgaagcg
    18901 atgttcctgt tcgctgaaac accgcacgct tacctgcaag gcgtggcgct tgaagtgatg
    18961 gcgaactccg ataacgtgct gcgtgcgggt ctgacgccta aatacattga tattccggaa
    19021 ctggttgcca atgtgaagtt cgaagccaaa ccggctaacc agttgttgac ccagccggtg
    19081 aaacaaggtg cagaactgga cttctcgatt cctgtggatg attttgcctt ctcgctgcat
    19141 gatcttagtg ataaagaaac caccattagc cagcaaagtg ccgccatttt gttctgcgtc
    19201 gaaggcgatg caacgctgtg tcaaggttct cagcagttac agcttaaacc gggtgaatca
    19261 gcgtttattg ccgccaacga atcaccggtg actgtcaaag gccacggccg tttagcgcgt
    19321 gtttacaaca agctgtaaga gcttactgaa aaaattaaca tctcttgcta agcttattaa
    19381 aggcttataa caccttcagg cggccagtcc gcctgatttc attttatgga taatcattat
    19441 gaataaatcg ctggtagcgg taggcgtcat tgttgcgcta ggcgtagtct ggacaggcgg
    19501 cgcatggtat acaggcaaga agattgaaac ccatctcgaa gacatggtcg cgcaggcgaa
    19561 cgcgcaactc aaactgacag ctcctgaatc caacctggaa gtgagttatc aaaactatca
    19621 tcgcggcgta ttcagcagcc agttgcaact gttggtgaaa cccattgccg ggaaagaaaa
    19681 tccgtggatt aaaagcggtc agagcgtcat cttcaacgaa tcggttgatc atggtccctt
    19741 cccccttgcc cagcttaaaa aactgaacct gatcccgtcg atggcatcaa ttcaaaccac
    19801 gctggtcaat aacgaagtaa gcaaaccact gtttgatatg gcaaaaggtg aaacgccttt
    19861 tgagattaac tcccgcattg gttacagcgg tgattccagt tccgatattt cgctcaatcc
    19921 actgaattac gagcaaaagg atgaaaaagt cgcctttagc ggcggcgagt tccagttaaa
    19981 tgctgacaga gacggcaaag ctatctccct ttccggggaa gcgcaaagtg gtcggataga
    20041 cgcagttaac gaatacaatc agaaagtgca gttgaccttt aataatctga aaaccgacgg
    20101 ttccagcacg ctggcaagtt ttggtgagcg cgtaggaaac caaaaactgt cactggaaaa
    20161 aatgaccatt tcagtggaag gcaaagaact ggcactgctg gaaggcatgg agatcagcgg
    20221 taaatcggat ctggtcaatg atggtaaaac gatcaatagc caactggatt actcgctaaa
    20281 cagcctgaag gtacagaatc aggatctggg cagcggcaag ctgactttaa aagtcggcca
    20341 aattgatggc gaagcctggc atcagtttag ccagcaatat aacgcgcaaa ctcaggcgct
    20401 gctggcacag ccagaaattg ccaacaatcc cgaactttat caggagaaag tgacggaagc
    20461 cttctttagc gccctgccgc tgatgctgaa aggcgatccg gtgattacta ttgcgccgct
    20521 aagctggaaa aacagtcagg gtgaaagtgc gctgaatctg tcgctgttcc tgaaagatcc
    20581 ggcaacgact aaagaagcgc cgcaaacgct ggcgcaggaa gtagatcgtt cggttaaatc
    20641 tctggatgcg aaactgacca ttccggtgga tatggcaact gagtttatga ctcaggtagc
    20701 gaagctggaa ggttatcagg aagatcaagc gaaaaaactg gcgaaacagc aagttgaagg
    20761 tgcatcagca atggggcaga tgttccgtct gaccaccttg caggacaata ccatcaccac
    20821 cagcctgcag tatgctaacg gtcagatcac gttaaacggg cagaaaatgc cactggaaga
    20881 ttttgttggg atgtttgcga tgccggcatt aaacgttccg gctgtacccg ctattccgca
    20941 gcagtaattc acagccaaaa cgccggaggt ttttctctcc ggcgttattt cttacttcag
    21001 cataaagtca tactgaatac tcattttcac atcccagtaa ttcaggcgat cgcccagcgc
    21061 actgccatcg cgcttttccc gccctttatc agcagcaaga aagaaattca tcccttgtaa
    21121 cggcccttcg ctgacccgat agttcatacc acctaagata cccgtgcctt caatccgcac
    21181 atctcgttta ctggattcat agccgcgagt cacaatcggt gcaaatgtca gtgtaaattg
    21241 cggcgttaaa cgatagttga cgcccaattg ccaggactgc attccttcac ggttacgatc
    21301 aagacttaat gaaaagggat agccaatgtc ggtatccacc acggcaccat tcattgcatg
    21361 gcgcaaataa ccaccactgc caaatacgct tccccacggc gcattccagg tcaattgtcc
    21421 actaaccaac gccgtttcat tgggctggct ggtattacga cttaaacctt cgagctgcgc
    21481 gtaaaagccg agcaactccc ctttcaacgt ataattcggg tcgagcggta tgcttttctc
    21541 gagaatgctt tgtatctgat tggaaacatc tttctgtacc gatgccccca gagaatagcg
    21601 actggtatca tcatgccaac tggcggctaa aaaagaacgt gcgcgatgtt tatcgtattt
    21661 tcccgtctgg ctgttataca ccccttcatc acgaataccg gttgttgaat cggtccataa
    21721 aaatactcgt gaacgccaca ttccccacag atcaatattt ggcgttaacg cccaataacc
    21781 taatgctcct tcagaaactg tcggtaatgt acgttgcgaa ctgccagcca ttaacggaat
    21841 acgataaggt aagctaccgt aactataatc cgtcccgaag cgacctaatt taagcaggct
    21901 attttcactg ggttttattt ttactgcccc cagggcaaag cgaaaacttt tatcaccatc
    21961 aagataccac cgggtactca tatcagcacg agcacctaat ttataaacat aatacgctcc
    22021 accttcaacg ccaataatat tggaataata accactattg aaatcgagca atcctccctg
    22081 tacccatgcg taaatatctc ttcccgcgcc agcactgggc ttatccgccc tgcgcaattc
    22141 gtttttcagt ctgatgcgta gtcccgcttc atcgtcagcc agttgcgccg cgtaagcaga
    22201 ggtaaggcca gaggcagccg tcaggcaaat aacggccatg gccactattt ttctcatagt
    22261 tgcacctttg atgttctgat tatttttatt gaatattaat tagtgatatc gctgattaat
    22321 tgctgctgca tttttttacg attatcaatt tcaaccacga tttctttgaa ttttttatcc
    22381 gtgagcggat aaaaccagat aataacgaat gccagtagca taaatccgca aggtactaag
    22441 gcaattgatg tgcggatgcc cataataact tccggcgttt gcacctgatt ggcgatatat
    22501 ccgcttaatc ccaaaataaa ggcaggaatt gaacctccga ttgcctgacc gcatttacgg
    22561 gtaaatgaga atagtgaata ggtgagccct tcaattcgca cgccggtcag gtattcaccg
    22621 tattctacgg tatcagcttc cagtacccac atcacagtca tagtaacgcc ctgaccaatt
    22681 gaagcgatgg ccaacgcaac caacgccacc ggcagtgacc agacggaaac ccagaagaat
    22741 agcagataac cgcaggttcc cagcaaagcg ccaatcagga aggtattctt tttaccgatc
    22801 ctcgcgacca tccccggcac cagcggtgcc gatgccacag taccgaccag gttttgccca
    22861 gtaccagcac agtaaacagc ccggtatcat ttaacacata gcgcacgtag aacaacgacg
    22921 aggcgctaac cgcgaaggtc gaaatcagca cacacagcgc accgatgcac aacataaaca
    22981 gcgggcgatt ccgtttcagg gtttgcagac tgatcttcaa tgacggctgc gccacgatac
    23041 gtaccacatt ctcacgcgtc gatttgaagc agatgaagta aagcaccatt ccggcaatcg
    23101 ccagcacaat cgtccagaaa tggtataccg acaccatctc ttccgggctg gagttcttaa
    23161 tgctcggtcc tatcagaaat gccaggcaga caaaggttaa tgaagcggca atcccgcgag
    23221 ccgcgcccag acgggcgcgg gattgtggtt gttgggtcat cgcggtagca agtgaaccat
    23281 aaggaatatt caccaggctg tagcaaagcc cgaggcccat gtaggtcaaa tatgcataca
    23341 ccactttgct accatggctc cagtcggtcg gcacccagaa taccagcacg ctgaagatca
    23401 ttaacggcgc agtaccgaag agtaaaaacg ggcggaattt tccccagcgg gtattcacac
    23461 tgtccaccac tcgtccggca aagacgtcgg cgaaggcatc gaatacccgc accagtaaca
    23521 gcatggtgcc cgccgcagcg gcaccgacgc cagcgacgtc ggtgtagtaa ctcaacagga
    23581 agagcgcccc cattgcgaag gcgaagttat tggcgacgtc accgaggctg tagccgacga
    23641 tggtgcgcca ggagagttgt tgattcattg tttgcctccc tgctgcggtt tttcaccaaa
    23701 gttcatgcca gtccagcgtt tttgcagcag aaaagccgcc gacttcggtt tgcggtcgcg
    23761 agtgaagatc cctttcttgt tacctccaac gcgcaatatg ccttgcgagg tcgcaaaatc
    23821 ggcgaaattc catacctgtt caccgacgac ggcgctgacg cgatcaaaga cgcggtgata
    23881 catatccagc catgcacact gatactcttc actccacatg tcggtgtaca ttgagtgcag
    23941 cccggctaac gtatccacgc cgtattcggt gatgataatc ggctgatgca gtttctcctg
    24001 ccaggccaga agttcttttt ccagtacctt ctctgccgtt tccaaatcgc cgctttggac
    24061 ataccaaccg taataacggt taaggcacag cacatcaaag agatcgctga tggtatcggt
    24121 gtgagcgtcg cagaacatta cattgacgca ggtgatcgga cgcgtcgggt cgagtttacg
    24181 cgttgcttcc gccagtggcg cgaaatattc tcgtgcacct tgcggacggg tatccggttc
    24241 gttggcaata ctccacatca ccacgcttgg gtggtttttg tcacgcgcta tcagctcttt
    24301 aatcgcctgt aagtgcgcct gctgagtttc cccgttgact gcctcttcgc tgtacagttc
    24361 tttcggcttg ttggccgctt cgaaaccaat gcctaaagag aggttaaagc cgacagcagc
    24421 agtttcatca atcaccacga tgccatgttc atctgcccag tcgagcatct cttcagcgta
    24481 agggtaatgc gaggtacggt aggagttggc cccaatccag tccattaatg cgtggtcgtg
    24541 caccatcagc acgttatcga atcctttgcc acgcaagtca gcatcttcat gacgaccaaa
    24601 gccagtaaag tagaacggtt tgtggttaat caggaactgt tcgcccttca ctgccactga
    24661 ccggatgccg acgcgaagcg ggtagatatc acactctgtc tggcttttgg ctgtgacgca
    24721 cagttcatag agataacctt cacccggttg ccagaggtgt ggattcacca cttgcaaagt
    24781 cccgctagtg ccttgtccag ttgcaaccac ctgttgatcc gcatcacgca gttcaacgct
    24841 gacatcacca ttggccacca cctgccagtc aacagacgcg tggttacagt cttgcgcgac
    24901 atgcgtcacc acggtgatat cgtccaccca ggtgttcggc gtggtgtaga gcattacgct
    24961 gcgatggatc ccggcataat taaagaaatc atggaagtaa gactgctttt tcttgccgtt
    25021 ttcgtcggta atcaccattc ccggcgggat agtctgccag ttcagttcgt tgttcacaca
    25081 aacggtgata cgcacacttt tcccggcaat aacatacggc gtgacatcgg cttcaaacgg
    25141 tgtatagccg ccctgatgct ccatcacttc ctgattattt acccacactt tgccgtaatg
    25201 agtgaccgca tcgaaacgca gcacgatacg ctggcctgcc caacctttcg gtataaagac
    25261 ttcgcgctga taccagacgt tgcccacata attacgaata tctgcatcgg cgaactgatc
    25321 gttaaaactg cctggcacag caattgcccg gctttcttgt aacgcgcttt cccaccaacg
    25381 ctgatcaatt ccgcagtttt cgcgatccag actgaatgcc cacaggccgt cgagtttttt
    25441 gatttcacgg gttggggttt ctacaggacg taacataagg gactcctcat taagataata
    25501 atactggtca acctttaatc tgattagtta accaattctg tgttgcacac agttatctgt
    25561 gagaaaccca tggaagagaa atggaaataa aaagaactga caccgcgatc aaaaaaccaa
    25621 aaactgcaat acccacagcg tgatatggat cgcattaatc tttaaaacag cgttgacaga
    25681 agccataaaa taagcaatcg aacgttcgtt cgagtatctt gagaacgata aactctcatg
    25741 acatatggct acggtgaata ttttgggggg ggaattctcc gatcccgggg agattgtatt
    25801 ttgtacaggg aaattaaagt atgaaacgcg ggcacagtta tcaccagccc gcgaaatgag
    25861 aggatcagga tgcggttaag ataccgccaa tcatcgcgcg taatccctga gcaaacgacg
    25921 cttcacgcgc cagatcttcc gcttcgatat cggccagaac accgtaggtc agatccagca
    25981 gtaaacgtga agtcattgcc gtgttgatgt ctgggcttat ctcgcctttt tgctgggcat
    26041 cacgcatccg ctgagcaaca aattcagtga tcgtcatatg cttattttta agcatggcgg
    26101 cgacctgtgg gttacgcatc ccttccgcca taatttcgac caccagcgcc cgttgcccaa
    26161 aggcttcatg ggtgagagag acaatagact cgaccatata gtcaacaaaa tgaatccctt
    26221 caatcggttc ccggaaacgg gccagcgccc tctcctggtc ctgtaagata atcgcctgaa
    26281 tcaaggcttc tttggagatg aaatggtgat agagcgtccc gggactaatg gcgcaagatt
    26341 tacagatggc tttcatcgag gcactgtgaa atccattttc tgaaaaaatc tctctggcag
    26401 cattgaggat ccgggtccgt gtcggttgtg cttcagtctg catgttatcc atcattaaac
    26461 cattatagtt agcgtgaata actagtttac cgcgttgtca ggtgagcgcc tatcattgct
    26521 gttgaatagt gcgaaggcac actctattcg tatgcttttc atccagctat tggttaacca
    26581 atagccgtga tttggctgcc agttgtttag cagagacgtt gatgaattgc acacgctatc
    26641 ccggtaaaca acacgtacgg ctgtttaccg gtccgttagt gtattaattg agctcctgta
    26701 ccccaccacc ggagactgtg agaatttgtc cgcttaccca gctggctgca ggcgagcaaa
    26761 ggaacagagc tgcgttagca atatcttgcg gttggcccag acgtctgatc ggcgtgtgct
    26821 gtaacatttt ttgttcaatt tctggtgtaa taacggattt cagggcatcg gttaatattg
    26881 cccccggcgc aatgccattt acccgaatat ttttttcacc caggtcaaac gccatatttc
    26941 tgaccagatg actggccgca gctttagatg atgcatagga tgtcatgttt atatttttat
    27001 tttctgccgc catagaagtg atggtcagaa taacgccacc gccatttttt tccatttctg
    27061 gcgcaacaag ttgtgacaga tggaaaaaag aaaacacatt cagttcataa gcacggcgaa
    27121 aatctgccat tggcatatca aacggtttag gaccaccgcc accggcgttg ttaaccagaa
    27181 tatcaacttt acccagctta ctgatagcaa agtctgccag tgcagagagt tcctgttcgg
    27241 aagtaatatc acaacggcag gcaaatgcct gaccacccag ttgttgaatt tcgtctacaa
    27301 catggttagc tgcgtcggcg ttaatatcac tgaccaccac agatgcgcca gctgtcgcga
    27361 atgtaatggc gatttcttta ccaatacctg cacccgcacc tgtgatgatg gcgcattttc
    27421 cgtcgagtct caggttgtca gaattaaaca cataaacctc ctgtgaactt cagtaaaaat
    27481 aaaaggcacc gcctgtgtgc cacatttttt atcattaacc ttgaggaaag gacactacca
    27541 ggtatagcag ataaagagag gattatttcg ccgcaatgag ccgggcggga ataataagat
    27601 tgcgtgaatg cgtctcttca tgggtgattt tttgcatcat gcgatccgca agtgtggtac
    27661 caagttcccg cgctggcgtg ctggcccagg taacaggtat atcatcaagt gtggttggtg
    27721 tcgcatcggt aaatgccgcc agcgaaacct gttgctcaaa gtaacgatcg actccgcttt
    27781 ccccgctttg ccgccctgct ttcagcaaac caaaccatgc ccccatcgca atagtttcgt
    27841 tatagcaaac cacggcactg atggtcgggt tatgacgtaa aagcgccgtg atagcttccg
    27901 ctgcttgctt ctggctggaa gtgcactcca acacccaatc gctgtgaaac ggcaggccaa
    27961 attttagtag agttgcacaa tagcccccca cccgttctgc acgggttaat gaggaacttt
    28021 gccctcccag ccaggcgatc cgctgatgcc cattgcgaat gagatgctcc gtcaacaact
    28081 gtgcagcctg catgttgtcc gggcgaaccg tatcaacatc atcaagataa ctggcacggg
    28141 aagcgaaaat caccgggata gctttttctt ctgccatccg tcgcaggtca tcgctgcttc
    28201 ctgcagcccc ggctattacc acaccatcga caccctgatt cagtaacagt gaaaaccgct
    28261 gtgccagctg ctcgccgtct ttaccgccgt gaagcaaaaa aaccatccgt ccctgcgctt
    28321 ccagagcttc cgtcaatccg gccgtcaatt cggcgtaaaa cggcgcagat aaatcacgga
    28381 cgatcaaacc aatgacgccg ctttgtccgc cgcgcagcgc cgacgcctgg cgattgcgca
    28441 caaatcccag ctcttcaatg gcggcgttaa cgcgttctcc tgtggcggta gagattcgcc
    28501 ctttgccact aagcaccagc gaaacggtac ttaccgacac gcccgcagcc agcgcaacat
    28561 catgaatggt tatttttttg gcggtagcca tgcagatgac ctactccctg attatgtgac
    28621 agataaaacg ttttaccttt tatttatctt atacccgcta ttatcgttgc gtaatgtgat
    28681 ttatgcctca ctaaaatttg ataaaacgtt ttatcttctc gcgcaattta ctgaatccag
    28741 attgttctct acgaggagtc gttttatgac ggcgaaaaca gcaccgaaag tcacgctgtg
    28801 ggagttcttc cagcagttag gcaaaacctt catgttaccc gtggcattat tgtcgttctg
    28861 cggcattatg ctcggcattg gcagttccct tagtagccac gatgtcatca ccctaatccc
    28921 ggtcctgggc aaccccgtgt tgcaggctat ctttacctgg atgagtaaga ttggctcgtt
    28981 tgcttttagt ttcctgcctg tcatgttctg tatcgccatc ccgctgggcc tggcacgcga
    29041 aaacaaaggc gtagcggcat tcgctggctt cgtcggttat gcggtaatga acctcgcggt
    29101 aaacttctgg ttgaccaata aaggcattct gccaaccacg gatgccgcgg ttctgaaagc
    29161 caataacatc cagagcattc ttgggatcca gtcgatcgat accgggatcc tcggtgcggt
    29221 gatcgccggt attatcgtct ggatgctgca tgagcgtttc cataatatcc gcctgccgga
    29281 tgcgctggca ttcttcggcg gtacgcgctt cgtaccaatt atctcctcgc tggtgatggg
    29341 ccttgtcggc ctggtgattc cattagtctg gccgattttc gccatgggta ttagcggctt
    29401 gggccatatg ataaacagcg cgggtgattt cggaccgatg ctgtttggta ccggtgaacg
    29461 tctgctgttg ccgtttggtc tgcatcacat tctggtggca ttaattcgct ttaccgacgc
    29521 aggcggcacg caggaagtct gcggtcaaac cgtcagcggc gcactgacca tcttccaggc
    29581 gcaattgaat tgcccgacca ctcacggttt ttctgaaagc gccacgcgtt tcctttcgca
    29641 aggtaaaatg cctgcgtttc tcggcggtct gccaggtgca gcgttagcta tgtatcactg
    29701 cgcgcgcccg gaaaatcgcc ataaaattaa aggtctgctg atttctggcc tgatcgcctg
    29761 cgtcgttggc ggcactaccg aaccgctgga attcctgttc ctgttcgtag cgccagttct
    29821 gtatgtcatc cacgcgctgt taaccggcct cggcttcacc gtcatgtctg tgctcggcgt
    29881 caccatcggt aataccgacg gcaatatcat cgacttcgtg gtgttcggta ttttgcatgg
    29941 tctgtcaacc aagtggtaca tggtgccagt ggtggcggca atctggtttg tcgtttacta
    30001 cgtcatcttc cgtttcgcta tcacccgctt caatctgaaa accccggggc gcgatagcga
    30061 agttgccagc tcaatcgaaa aagccgttgc cggtgcgccg ggtaaatcag gttacaacgt
    30121 tcctgcaatc ctcgaagcat taggcggtgc cgacaatatt gtcagcctcg ataactgcat
    30181 tacccgtctg cgtttgtctg tgaaagatat gtcgcttgtt aatgtgcagg cactgaagga
    30241 caatcgggca attggcgtag tacaacttaa tcaacataac ctgcaggttg ttatcgggcc
    30301 acaagttcag tcagtaaaag atgaaatggc cggtctgatg catactgtcc aggcataagg
    30361 atacgatatg ttcgattttt caaaggtcgt ggatcgtcat ggcacatggt gcacgcagtg
    30421 ggattatgtc gctgaccgtt tcggcactgc tgacctgtta ccgttcacga tttcagacat
    30481 ggattttgcc actgccccct gcattatcga ggcgctgaat cagcgcctga tgcacggcgt
    30541 atttggctac agccgctgga aaaacgatga gtttctcgcg gctattgccc actggttttc
    30601 cacccagcat tacaccgcaa ttgatacgca gacggtggtg tatggccctt ctgtcatcta
    30661 tatggtttca gaactgattc gtcagtggtc tgaaacaggt gaaggcgtgg tgatccacac
    30721 gcccgcctat gacgcatttt acaaggccat tgaaggtaac cagcgcacag taatgcccgt
    30781 tgctttagag aagcaggctg acggctggtt ttgcgatatg ggcaagctgg aagccgtgtt
    30841 ggcgaaacca gaatgtaaaa ttatgctcct gtgtagccca cagaatccta ccggtaaagt
    30901 gtggacgtgc gatgagctgg agatcatggc tgacctatgc gagcgtcatg gtgtgcgggt
    30961 tatttccgat gaaatccata tggatatggt ctggggcgag cagccgcata ttccctggag
    31021 taatgtggcg cggggagact gggcgttgct gacgtcggga tcgaaaagtt tcaatattcc
    31081 cgcgctgacc ggtgcttacg ggattataga aaatagcagc agccgtgatg cctatttatc
    31141 ggcactgaaa ggacgtgatg ggctttcttc cccttcggta ctggcgttaa ctgcccatat
    31201 cgccgcctat cagcaaggtg caccgtggct ggatgcctta cgcatctatc tgaaagataa
    31261 cctgacgtat atcgcagata aaatgaacgc cgcatttcct gaactcaact ggcagatccc
    31321 gcaatccact tatctggcat ggcttgattt acgtccgttg aatattgacg acaacgcgtt
    31381 gcaaaaagca cttatcgaac aagaaaaagt cgcgatcatg ccggggtata cctacggtga
    31441 agaaggtcgt ggttttgtcc gtctcaatgc cggctgccca cgttcgaaac tggaaaaagg
    31501 tgtggctgga ttaattaacg ccatccgcgc tgttcgttaa ccccaattgc gcaacgtaaa
    31561 aaatcgttgc gcaatcgtgg atttttaccc tgctttgttt ttataatggt gcgcactttt
    31621 atatccagaa aaagagtgcg accatgattg ataccaccct gccattaact gatatccatc
    31681 gccaccttga tggcaacatt cgtccccaga ccattcttga acttggccgc cagtataata
    31741 tctcgcttcc tgcacaatcc ctggaaacac tgattcccca cgttcaggtc attgccaacg
    31801 aacccgatct ggtgagcttt ctggccaaac ttgactgggg cgttaaagtt ctcgcctctc
    31861 ttgatgcctg tcgccgcgtg gcatttgaaa acattgaaga tgcagcccgt cacggcctgc
    31921 actatgtcga gctgcgtttt tcaccaggct acatggcaat ggcacatcag ctgcctgtag
    31981 cgggtgttgt cgaagcggtg atcgatggcg tacgtgaagg ttgccgcacc tttggtgtgc
    32041 aggcgaagct tatcggcatt atgagccgga ccttcggcga agccgcctgt cagcaagagc
    32101 tggaggcctt tttagcccac cgtgaccaga ttaccgcact tgatttagcc ggtgatgaac
    32161 ttggtttccc gggaagtctg ttcctttctc acttcaaccg cgcgcgtgat gcgggctggc
    32221 atattaccgt ccatgcaggc gaagctgccg ggccggaaag catctggcag gcgattcgtg
    32281 aactgggtgc ggagcgtatt ggacatggcg taaaagccat tgaagatcgg gcgctgatgg
    32341 attttctcgc cgagcaacaa attggtattg aatcctgtct gacctccaat attcagacca
    32401 gcaccgtagc agagctggct gcacatccgc tgaaaacgtt ccttgagcat ggcattcgtg
    32461 ccagcattaa cactgacgat cccggcgtac agggagtgga tatcattcac gaatataccg
    32521 ttgccgcgcc agctgctggg ttatcccgcg agcaaatccg ccaggcacag attaatggtc
    32581 tggaaatggc tttcctcagc gctgaggaaa aacgcgcact gcgagaaaaa gtcgccgcga
    32641 agtaacaaaa tggatggtgc aaattgcacc atccattttt catgcaaggc acaaagtcgc
    32701 gcgatgtttg gcggattcga tgcccagctc aatcaactcc attacctgga ttgcctggct
    32761 tgccggaacc ggattttcac catcgccatt taacgcatca cgaatagccg catagtaagc
    32821 cggataattc ccaggcaccg tcaacagcgt ttcttcgaca cgttcctcac cttccacgcg
    32881 ggtaagtacg ccatcacgca tatcgtagcc ccagtcttcc tgcggtagac gctcgccatt
    32941 tttcagacgt tcttcctgtg gatcgaggcc atatttcaca taactgcctc gggatccatg
    33001 cacgatatac cgtgctgact cagcggctgc cagcatggta ccgtgtaaaa tgactcgccg
    33061 ctgtggatag gacaagatgg cgtggaaata atcggtcgac tgcgctccgg gccgtaactg
    33121 tgccaaatct accgtcatgc tgaccggtaa accaaaaagc gtaatggcct gatcaagaag
    33181 atgtggtgct aaatcgtacc agataccgct gcctgggccg ccctgttcac gccaacgatc
    33241 gcgcacctgc ggacggaagc ggtcaaaatg agactcaaag taagcaactt cacccagcac
    33301 gccttccgcg agtaaacctt ttagcgtcaa gaaatcgcta tcccagcgac ggttatggaa
    33361 tacagacagc acacgcccca ggctttttgc cagcgcatcc agctctcgcg cttgtgacag
    33421 tgtcacggta aagggtttat caacgaccac atgtttaccc gcctcaagcg ccgctttggc
    33481 taacgggaaa tgggtatcgt tgggtgtagg aatgacaatc aggtctatgt tgggatcgtt
    33541 aaacagatgc ttcggctcag agacaaccgt aaccgttggc cagtcggctt ttacttttgt
    33601 ttcatcgctg ctggagatta ccgccagttc cagcccgggc gtgcccgcaa tcaggggcgc
    33661 atggaaggtt ttgctcgcat aaccataccc aatcaaccca acacggatgt tgtcgctcat
    33721 gtcattgcct ctcattgaag tatgatggct atttgacact atcctttacc cacgctcaac
    33781 agtttaataa cctgccagca ataagggatg ttgtttaact taagtcaaaa aaatagcgaa
    33841 ttttccaacg acaaaagcta aatatcgcaa aaacctcagt aaaaatcttg ctggagctat
    33901 tattgctaag taacatttac cccctgaagt taatggatca atcaagagag atgtgggctg
    33961 taatgaatcg tcttattgaa ttaacaggtt ggatcgttct tgtcgtttca gtcattcttc
    34021 ttggcgtggc gagtcacatt gacaactatc agccacctga acagagtgct tcggtacaac
    34081 acaagtaagc tctgcacttg tggagcgaca tgctgcccgt ccgggtgcat gttttcactt
    34141 gtcggatatt aaaccaggaa tttattatct tgttcgatgt tgttggtgat tgtcagggat
    34201 agtaaagcgc gttactcttt tcagagcagt ttttcatccc tgaatatttg ccatcagcgc
    34261 attcttatta ttgtaataac acaagcgttc acttttggtg aaattttatc atcactaata
    34321 atacctaccc ctttggtatt aattatttta tggacctttt atgactgttc aggactactt
    34381 attaaaattt cgcaaaatca gttcactcga aagtctggaa aaactctacg accatcttaa
    34441 ttacaccctg acggacgatc aggaactgat caatatgtat cgtgctgccg atcaccgtcg
    34501 cgcagagctg gtttctggcg ggcgtttgtt tgacctcggc caggtaccga agtccgtctg
    34561 gcactatgtc caataaagaa agtagcgatc ggttataaaa gccttataat aacgccccta
    34621 aaattagatt tgcctgtttg cgtcgctatt ctggagaacc tatgaccacc acaacgccac
    34681 aacgtattgg aggctggttg cttggcccgt tggcctggct tttagtcgct ctgttgagta
    34741 cgacgctggc gttgttgctg tataccgctg cgttatcttc tccgcaaaca tttcaaacgc
    34801 tcggcggaca agcactgacg acacaaatct tatggggcgt ttcttttatt accgctatcg
    34861 ccatgtggta ttacacactg tggttgacca tcgctttctt taaacgtcgg cgttgtgttc
    34921 ctaaacacta tattatctgg ctgcttattt ccgtattgct ggcagtaaaa gcattcgctt
    34981 tttcacccgt ggaagatggc atagccgttc gccagttgct gtttactttg ttggcaaccg
    35041 cattgatcgt cccctatttc aaacgctcgt cgcgggtaaa agcgacgttt gtgaatccgt
    35101 aataacctta cagttaacct gttgtcgcct gctctggatt aacggataat aggcggcttt
    35161 tttatttcag gccgaaaaat gactgactac ctgttactgt ttgtcggaac tgtactggtc
    35221 aataactttg tactggtcaa gtttctcggt ctctgtccgt ttatgggggt ttccaaaaaa
    35281 ctggaaaccg cgatgggcat ggggctggca acaacgtttg tgatgacgct ggcgtctatt
    35341 tgcgcctggc ttatcgatac gtggattttg atcccactta atctgattta cttgcgcacc
    35401 ctggcattta ttctggtgat tgctgtggtc gtgcagttca ccgagatggt ggtgcgcaaa
    35461 accagcccgg tgctttaccg tttgctgggg atttttttgc cgcttatcac caccaactgt
    35521 gccgtgctcg gcgtggcgtt gctgaatatc aatctcgggc acaatttctt gcagtcggcg
    35581 ctgtacggtt tttccgccgc tgtcggtttt tcgctggtga tggttctctt tgcagccatc
    35641 cgcgaacgcc ttgctgtggc tgatgtcccg gcaccttttc gcggtaatgc cattgcgtta
    35701 attaccgcag gtcttatgtc tctggccttt atgggcttta gtggtttggt gaagttgtaa
    35761 tgaatgctat ctggattgcc gttgccgccg tgagcctgct gggcctggcg tttggcgcca
    35821 ttctgggtta tgcctcccgc cgttttgcgg tggaagacga tccggtcgtt gagaaaattg
    35881 acgaaatctt accgcagagc cagtgtggtc agtgcggtta tcccggctgt cgcccctacg
    35941 cggaagccat cagctgtaac ggtgaaaaaa tcaaccgttg cgccccaggt ggcgaagctg
    36001 tgatgctaaa aattgccgag ttgcttaatg tcgagccgca gccgctggat ggcgaagcgc
    36061 aagagctaac gcctgcgcgg atggtggcgg ttattgatga aaataactgt attggctgca
    36121 ctaaatgtat tcaggcgtgt ccggtagacg ccatcgttgg cgctacccgt gccatgcata
    36181 cggtaatgag tgatctctgt acgggctgca atttatgtgt tgatccgtgc ccgacgcact
    36241 gcatctcgtt gcaaccggtc gcagaaacac ctgactcctg gaaatgggat ctgaacacca
    36301 ttcccgtgcg tatcattccc gtggaacacc atgcttaagt tattctctgc attcagaaaa
    36361 aataaaatct gggatttcaa cggcggcatc catccaccgg agatgaaaac ccagtccaac
    36421 ggtacaccac tgcgccaggt accactggcg cagcgttttg ttattccact gaaacagcat
    36481 attggcgctg aaggtgagtt gtgcgttagc gtcggcgata aagtattgcg cggccagccg
    36541 cttacccgtg gtcgcggcaa aatgctgcct gttcacgcgc ccacctcggg taccgttacg
    36601 gctattgcgc cccactctac ggctcatcct tcagctttag ctgaattaag cgtgattatt
    36661 gatgccgatg gtgaagactg ctggatcccg cgcgacggct gggccgatta tcgcactcgc
    36721 agtcgcgaag agttaatcga gcgcatacat cagtttggtg tagccgggct gggcggtgca
    36781 ggattcccga caggcgttaa attgcagggt ggcggagata agattgaaac gttgattatc
    36841 aacgcggctg agtgcgagcc gtacattacc gccgatgacc gtttgatgca ggattgcgcg
    36901 gctcaggtcg tagagggtat tcgcattctt gcgcatattc tgcagccacg cgaaattctt
    36961 atcggcattg aagataacaa accgcaggcg atttccatgc tgcgcgcggt gctggcggac
    37021 tctaacgata tttctctgcg ggtgattcca accaaatatc cttctggcgg tgctaaacaa
    37081 ttaacctaca ttctgaccgg gaagcaggtt ccacatggcg ggcgttcatc cgatatcggc
    37141 gtattaatgc aaaacgtcgg cactgcttat gcagtgaaac gtgccgttat tgatggcgag
    37201 ccgattaccg agcgtgttgt aaccctgact ggcgaagcaa tcgctcgccc gggcaacgtc
    37261 tgggcacggc tggggacgcc agtgcgtcat ttattgaatg atgccggatt ctgcccctct
    37321 gccgatcaaa tggtgattat gggtggcccg ctaatgggct ttaccttgcc atggctggat
    37381 gtcccggtcg taaagattac caactgtctg ttggctccct ctgccaatga acttggcgaa
    37441 ccacaggaag aacaaagctg catccggtgt agcgcctgtg ctgacgcctg ccctgcggat
    37501 cttttgccgc aacagttgta ctggttcagc aaaggtcagc aacacgataa agctaccacg
    37561 cataacattg ctgattgcat tgaatgtggg gcttgcgcgt gggtttgccc gagcaatatt
    37621 cccctggtgc aatatttccg tcaggaaaaa gctgaaattg cggctattcg tcaggaagaa
    37681 aagcgcgccg cagaagccaa agcgcgtttc gaagcgcgcc aggctcgtct ggagcgcgaa
    37741 aaagcggctc gccttgaacg acataagagc gcagccgttc aacctgcagc caaagataaa
    37801 gatgcgattg ctgccgctct ggcgcgggtg aaagagaaac aggcccaggc tacacagcct
    37861 attgtgatta aagcgggcga acgcccggat aacagtgcaa ttattgcagc acgggaagcc
    37921 cgtaaagcgc aagccagagc gaaacaggca gaactgcagc aaactaacga cgcagcaacc
    37981 gttgctgatc cacgtaaaac tgccgttgaa gcagctatcg cccgcgccaa agcgcgcaag
    38041 ctggaacagc aacaggctaa tgcggaacca gaagaacagg tcgatccgcg caaagccgcc
    38101 gtcgaagccg ctattgcccg tgccaaagcg cgcaagctgg aacagcaaca gtctaatgcg
    38161 gaaccagaag aacaggttga tccgcgcaaa gccgccgtca aagccgctat tgcccgagcc
    38221 aaagcgcgca aactggaaca gcaacaggct aatgcggaac cagaagaaca ggttgatccg
    38281 cgcaaagccg ccgtcgaagc cgctattgcc cgtgccaaag cgcgcaagct ggaacagcaa
    38341 cagactaacg cagaaccaga agaacaggtt gatccgcgca aagcggcagt tgccgcggct
    38401 attgcccgcg ctcaggccaa aaaagccgcc cagcagaagg ttgtaaacga ggactaaatg
    38461 gtattcagaa tagctagctc cccttatacc cataaccagc gccagacatc gcgcattatg
    38521 ctgttggtgt tgctcgcagc cgtgccagga atcgcagcgc aactgtggtt ttttggttgg
    38581 ggtactctcg ttcagatcct gttggcatcg gttagtgctc tgttagccga agctctcgta
    38641 ctcaaactac gcaagcagtc ggtagccgca acgttgaaag ataactcagc attgctgaca
    38701 ggcttattgc tggcggtaag tattcccccc ctcgcgccat ggtggatggt cgtgctgggt
    38761 acggtgtttg cggtgatcat cgctaaacag ttgtatggcg gtctggggca aaacccgttt
    38821 aatccggcaa tgattggtta tgtggtctta ctgatctcct tcccggtgca gatgaccagc
    38881 tggttaccgc cacatgaaat tgcggtcaac atccctggtt ttatcgacgc catacaggtt
    38941 attttcagcg gacataccgc cagtggtggt gatatgaaca cactacgctt aggtattgat
    39001 ggcatta
//
LOCUS       DAMZET010000024        37988 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.024, whole genome shotgun sequence.
ACCESSION   DAMZET010000024 DAMZET010000000
VERSION     DAMZET010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.024"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1394
                     /gene="sthA"
                     /locus_tag="POB34_001352"
     CDS             <1..1394
                     /gene="sthA"
                     /locus_tag="POB34_001352"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HDI5918336.1"
                     /translation="SYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGGCTH
                     WGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQGFY
                     ERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTHPRI
                     YDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQEMSD
                     SLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTDSLA
                     LQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALVKGE
                     ATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMNVGT
                     LKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTMAEA
                     YRVAALNGLNRLF"
     gene            complement(1377..2294)
                     /gene="oxyR"
                     /locus_tag="POB34_001353"
     CDS             complement(1377..2294)
                     /gene="oxyR"
                     /locus_tag="POB34_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HDI5918337.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2546..3829)
                     /locus_tag="POB34_001354"
     CDS             complement(2546..3829)
                     /locus_tag="POB34_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5918338.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPLALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            complement(3896..5110)
                     /locus_tag="POB34_001355"
     CDS             complement(3896..5110)
                     /locus_tag="POB34_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HDI5918339.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            complement(5607..6980)
                     /gene="argH"
                     /locus_tag="POB34_001356"
     CDS             complement(5607..6980)
                     /gene="argH"
                     /locus_tag="POB34_001356"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDI5918340.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(7041..7817)
                     /gene="argB"
                     /locus_tag="POB34_001357"
     CDS             complement(7041..7817)
                     /gene="argB"
                     /locus_tag="POB34_001357"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDI5918341.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(7825..8829)
                     /gene="argC"
                     /locus_tag="POB34_001358"
     CDS             complement(7825..8829)
                     /gene="argC"
                     /locus_tag="POB34_001358"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDI5918342.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            8983..10134
                     /gene="argE"
                     /locus_tag="POB34_001359"
     CDS             8983..10134
                     /gene="argE"
                     /locus_tag="POB34_001359"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HDI5918343.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            10486..13137
                     /gene="ppc"
                     /locus_tag="POB34_001360"
     CDS             10486..13137
                     /gene="ppc"
                     /locus_tag="POB34_001360"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HDI5918344.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            13320..15053
                     /locus_tag="POB34_001361"
     CDS             13320..15053
                     /locus_tag="POB34_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HDI5918345.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSKQTDKQFYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     NPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            15202..16053
                     /locus_tag="POB34_001362"
     CDS             15202..16053
                     /locus_tag="POB34_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5918346.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(16040..16381)
                     /locus_tag="POB34_001363"
     CDS             complement(16040..16381)
                     /locus_tag="POB34_001363"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDI5918347.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLGKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(16383..17261)
                     /locus_tag="POB34_001364"
     CDS             complement(16383..17261)
                     /locus_tag="POB34_001364"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HDI5918348.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(17227..19524)
                     /locus_tag="POB34_001365"
     CDS             complement(17227..19524)
                     /locus_tag="POB34_001365"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDI5918349.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGTRYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(19575..19895)
                     /locus_tag="POB34_001366"
     CDS             complement(19575..19895)
                     /locus_tag="POB34_001366"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDI5918350.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(19910..20989)
                     /locus_tag="POB34_001367"
     CDS             complement(19910..20989)
                     /locus_tag="POB34_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDI5918351.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLISRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            21298..23799
                     /gene="ptsP"
                     /locus_tag="POB34_001368"
     CDS             21298..23799
                     /gene="ptsP"
                     /locus_tag="POB34_001368"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDI5918352.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            23811..24473
                     /gene="fsa"
                     /locus_tag="POB34_001369"
     CDS             23811..24473
                     /gene="fsa"
                     /locus_tag="POB34_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDI5918353.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            24484..25587
                     /gene="gldA"
                     /locus_tag="POB34_001370"
     CDS             24484..25587
                     /gene="gldA"
                     /locus_tag="POB34_001370"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HDI5918354.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            25862..26479
                     /locus_tag="POB34_001371"
     CDS             25862..26479
                     /locus_tag="POB34_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HDI5918355.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(26506..27411)
                     /gene="yijE"
                     /locus_tag="POB34_001372"
     CDS             complement(26506..27411)
                     /gene="yijE"
                     /locus_tag="POB34_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HDI5918356.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRAMRPTPFKYTLAIALLQTCGMIGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALVYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGVVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(27505..29685)
                     /gene="katG"
                     /locus_tag="POB34_001373"
     CDS             complement(27505..29685)
                     /gene="katG"
                     /locus_tag="POB34_001373"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HDI5918357.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(30014..30904)
                     /gene="metF"
                     /locus_tag="POB34_001374"
     CDS             complement(30014..30904)
                     /gene="metF"
                     /locus_tag="POB34_001374"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDI5918358.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(31253..33685)
                     /gene="metL"
                     /locus_tag="POB34_001375"
     CDS             complement(31253..33685)
                     /gene="metL"
                     /locus_tag="POB34_001375"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HDI5918359.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(33688..34848)
                     /gene="metB"
                     /locus_tag="POB34_001376"
     CDS             complement(33688..34848)
                     /gene="metB"
                     /locus_tag="POB34_001376"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDI5918360.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            35125..35442
                     /gene="metJ"
                     /locus_tag="POB34_001377"
     CDS             35125..35442
                     /gene="metJ"
                     /locus_tag="POB34_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HDI5918361.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            35626..36234
                     /locus_tag="POB34_001378"
     CDS             35626..36234
                     /locus_tag="POB34_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HDI5918362.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            36379..37054
                     /locus_tag="POB34_001379"
                     /pseudo
     CDS             36379..37054
                     /locus_tag="POB34_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(37141..37464)
                     /locus_tag="POB34_001380"
     CDS             complement(37141..37464)
                     /locus_tag="POB34_001380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918363.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFLSNSDSAALYCVAKIF
                     PDGENILIYKVNIINASSKKDVFFYESPTSTHNDIMGGGGMSYAYEYDVKYNICHLRK
                     KSRMR"
     gene            complement(37466..37858)
                     /locus_tag="POB34_001381"
     CDS             complement(37466..37858)
                     /locus_tag="POB34_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HDI5918364.1"
                     /translation="MCNGESINENKEYGGLICKKQGEYFPMNPISSNDNDSVDLRNIK
                     CPEGSERVGDYHTHGFYSDDKGNKVTKENDVYDSLNFSSKDLTNSYMNGMGKKEYSSY
                     LGTPNNTYLKYNPKAKGNGVTIIRQGSN"
BASE COUNT         9394 a        10483 c         9382 g         8729 t
ORIGIN      
        1 attcctacga ttacgatgcc atagtaatag gttccggccc cggcggcgaa ggcgctgcaa
       61 tgggcctggt taagcaaggt gcgcgcgtcg cagttatcga gcgttatcaa aatgttggcg
      121 gcggttgcac ccactggggc accatcccgt cgaaagctct ccgtcacgcc gtcagccgca
      181 ttatagaatt caatcaaaac ccactttaca gcgaccattc ccgactgctc cgctcttctt
      241 ttgccgatat ccttaaccat gccgataacg tgattaatca acaaacgcgc atgcgtcagg
      301 gattttacga acgtaatcac tgtgaaatat tgcagggaaa cgctcgcttt gttgacgagc
      361 atacgttggc gctggattgc ccggacggca gcgttgaaac actaaccgct gaaaaatttg
      421 ttattgcctg cggctctcgt ccatatcatc caacagatgt tgatttcacc catccacgca
      481 tttacgacag cgactcaatt cttagcatgc accacgaacc gcgccatgta cttatctatg
      541 gtgctggagt gatcggctgt gaatatgcgt cgatcttccg cggtatggat gtaaaagtgg
      601 atctgatcaa cacccgcgat cgcctgctgg catttctcga tcaagagatg tcagattctc
      661 tctcctatca cttctggaac agtggcgtag tgattcgtca caacgaagag tacgagaaga
      721 tcgaaggctg tgacgacggt gtgatcatgc atctgaagtc gggtaaaaaa ctgaaagctg
      781 actgcctgct ctatgccaac ggtcgtaccg gtaataccga ttcgctggcg ttacagaaca
      841 ttgggctgga aactgacagt cgcggacagc tgaaggtcaa cagcatgtat cagaccgcac
      901 agccgcacgt ttacgcggtg ggcgacgtga ttggttatcc gagcctggca tcagcggcct
      961 atgaccaggg gcgcattgcc gcgcaggcgc tggtaaaagg cgaagccacc gcgcatctga
     1021 ttgaagatat ccctaccggc atttacacca tcccggaaat cagctctgtg ggcaaaaccg
     1081 aacagcagct gaccgcgatg aaagtgccat atgaagtggg ccgcgcccag tttaaacatc
     1141 tggcacgcgc acaaatcgtc ggcatgaacg tgggcacgct gaaaattttg ttccatcggg
     1201 aaacaaaaga gattctgggt attcactgct ttggcgagcg cgctgccgaa attattcata
     1261 tcggtcaggc gattatggaa cagaaaggtg gcggcaacac tattgagtac ttcgtcaaca
     1321 ccacctttaa ctacccgacc atggcggaag cctatcgggt agctgcgtta aacggtttaa
     1381 accgcctgtt ttaacacttt atcgaaatgg ccatccattc ttgctcggat ggcctctgcc
     1441 agctgctcat agcggctgcg cagcggtgag ccaggacgat aaaccaggcc aatagtgcgg
     1501 cgtggttccg gcttaatgca cggcagataa acaaccccat cgcgtttgcg ctccggcggc
     1561 acagccagcg ctggcagtaa agtgatcccg ctacctgccg ccaccatgtt gcgcagcgtc
     1621 tccaggctgg tcgcgcggaa gtgtgtatct tcatccgccc cggcttcaaa acagaaaccc
     1681 attgcctgat cgcgcaaaca gtgaccatct tccagcatca gcagtttttc ccctgccaga
     1741 tcggccatcg gtacgcattc gcggttcgcc cacgggtgat cttcatagat agccagcaac
     1801 attggctcat caaacaacgg cacttcaatg aatgcttcgc tctctttcac cagcgcgagg
     1861 atcacgcaat cgagtttgcc gctgtccagt tgcgccagta actggtgggt ctgtgcttca
     1921 tgcagataca tttccagctt tggaaaggtc tggtgcagca tagggataat atgcggtagc
     1981 aggtacggtc caactgtggg aatcaaacca atgtgcagcg gcccggacat cgtctcgccc
     2041 tgctggcttg ccatctcttt aaggactttc acctcacgca gtacggtacg cgcctgatct
     2101 accagcagca ttcccgcctg ggtgaacaac actttacggc tggtccgctc cagcaacatc
     2161 acgcccaact catcttccag cttacgaatt tgcccgctaa gcgtcggctg gctaacgtgg
     2221 caggaatctg ccgcacgccg aaaatggcgg tgctcggcca atgccaccag gtactcaaga
     2281 tcacgaatat tcattatcca tcctccatcg ccacgatagt tcatggcgat aggtagaata
     2341 gcaatgaacg attatcccta tcaagcattc tgactgataa ttgctcacag aaacggagcg
     2401 gcacctcttt taacccttga agtcactgcc cgtttcgaga gtttctcaac tcgaataact
     2461 aaagccaacg tgaacttttg cggatctcca ggatccgctt ttttttattc tacaggccca
     2521 cagcaagaga gcctgatagt aacgattact caatgcgatc tttgacaata aagaagtaac
     2581 acagcgcccc ggtgaacgtc acacaggccg cgataaccaa cgccagatta aacgagtgtg
     2641 tggtatccac tacccaaccg gttacgatcg gagcaaagga agcaaaaaca aaactgccaa
     2701 agttttgaat cccggcgatg gaagccactt ttgtttctga caccatcacc tgcaccagcc
     2761 cccatgcaga cgttccagcg aaatgcacgc agaatagcgc cattgagata aacgctaccg
     2821 cctgggctgg cgaggaagat tgcacgacca gcaacgtgcc taatgccgac atcatcaaac
     2881 cgcagacaat agccgtttta cgcgtcttcg ccaggtcgta gccttttttc gccagtcgat
     2941 cgacaacaat accgttaacc cacatcccga ctgccgccgc gaggaaagga atcgccgcca
     3001 cccaaccggt tttcgccaga ctgaaacctt gctcggcttg caaataaccg ggtaaccacg
     3061 cgatgtagag ccaaccggta taattgacgc cagagaaacc taaaatcatc ccccaggttg
     3121 tccgatgctt aaacagcgcc agccactcgc taaattgcag ctgtggacgc ggtttaaccg
     3181 gcgcggagag atacgtccgt tcttcgtcag tcaggacaaa ctgcgcccgg ttgcgatacc
     3241 acgcgtacca acagatccca accagaatcc ctgccacgcc gatgataacg aacatcgtcc
     3301 gccagcccca tgccagctgc atcaatacaa gagcaagagg cgcgatagcc tgaccgataa
     3361 cggtagatga gttaaaaatc cccagcgccg tgccgcgttc tttttgtgca taccagtcgg
     3421 tgatcgactt tactcccgca ggcataaatg gcgcttcgcc aatacccaga ccgatacgca
     3481 ttaaaataaa gtggctaaaa gaattaacca ttcctgttaa tgcctgcatt aatgaccaga
     3541 atattaagcc tgcgcctaaa acaattcgtg ggccaaagcg gtctagcaaa ataccggaag
     3601 gtagttgtga aaaaccataa gacagagaaa atgcagaaag caaaacacca aactctgtcg
     3661 ccgataatcc taactcgcca cgaatcgctt cacctgccac gctcaatgac gaacggtcga
     3721 gaaaattaac gatccccgcc ataaataata aaaccagggt cactgtctga atacgacgaa
     3781 tccgttgcgg tttattaccc gcaacactgg ttgacacatc aacgtccatt tttagctcct
     3841 tgagcggaat taatcgattt gatgactatt cagaaaataa ttaagcgaac cgttattacc
     3901 agttccacaa tgtgccatct tccagtcgtg cgacggggag atacgccggg tcatacggat
     3961 atttcgccgc cagtttctca tcgaaagaaa tacccagccc cggttcatcg cctgggtgca
     4021 tataaccttc ctcgaagcgc cagctgtgcg ggaaaacttc aagcatttgt tcggaatagc
     4081 ccatgtactc ctgaacgcca aaatttggta cccataggtc aaaatgcagc gccgccgcat
     4141 gacaaacagg cgataaatct gaagggccat gcgaaccggt acgtacctga taaagcgatg
     4201 caaaatcggc aatccgccgc atcccggtaa tgccgcctgc atgggtgatc gtcgtgcgaa
     4261 tatagtcaat cagctgctct tcgattaact gcttgcagtc ccagatactg ttaaacactt
     4321 caccgaccgc aatcggcgtc acggtatgct ggcgaatcag acgaaaacat tcctggttct
     4381 ctgcaggcgt cggatcttcc atccagaaca ggcggtaatc ctcaatgctc ttcccaaagc
     4441 gcgccgcttc gatgggggtc aggcgatggt gcatgtcatg caacagatgt tcgttaaaac
     4501 cgaacttatt acgcaccgct tcaaataact tcggcgtaaa atcgagatac ttttctgaag
     4561 accacaactg ctcttctggc cacaggcctt tggttgccgg ttcatacgcc aggcctttgc
     4621 ccttcgccag gccataagta gtttgcatac ccggtacacc gcactgcaca cgaatcgcct
     4681 taaagcccat ctgctggtgc tttgcatagt cttcaaggac ttcatcaatc gtccgcccgg
     4741 tggtgtggca ataaaccatc acgccttcac gagaagcacc accaagcagt tgatacaatg
     4801 gcatatttgc cgctttagct ttgatatccc acaacgccat atcaatggca gaaatggctg
     4861 acatcgtgac ggggccacgt cgccagtaag cacctttata aaagaattgc cagatatctt
     4921 cgatacggct ggcatcgcgg ccaattagtt gtggacaaag atgatctttc agataagaag
     4981 cgaccgacaa ttcgcgacca tttaacgtgg catctcctaa tccacaaagt ccgctttcag
     5041 tggttatttt aagcgtaacg aaattacgcc ctgggcaagt aacaaaaaca tctgcagcaa
     5101 taattttcat tattgcaatc tcctgtgttg taaatagacg cttcaaactt aattttacgc
     5161 ctactaccat acaagagaga aagagcgatt attcaaccgt gatcacatta aaaataaaaa
     5221 tttccgccga ggagaaaaaa taccctcaaa aaccacctga ataattaacc acatcgttaa
     5281 aacaacacgt taaataatca cataacaaac tccattaatc attaaaaata agcacaacaa
     5341 tagaaatgaa ttatattcaa ttaattaaaa tatgtattga ttgatgtatt gaaaatatga
     5401 tttttatggg gagtgtgaag gtaaagaaga cacaatgata aaaaggaatg tgacaaataa
     5461 tggttctggt tgggcgttaa atttaagctc accagaatgc tgacaaattt ctgcctgatg
     5521 tggcgtaaac gccttatccg cctacgaagt gtcgcacttt gtaggcgtgg taagctcccc
     5581 attttcaggg gagcaatata aacaaattac tctaaccgcg cctgcgcaaa agcaattgcc
     5641 tgcgccacct gctgcggtga gacgccgcct tttgccgcac gcttgtcgag gcacgattgc
     5701 agcgacagaa tcggatagac atcttcgtca atcaccgggc tgaatttctg caactcgtcg
     5761 agcggcagat cttccagcgg tttgccctga cgaatggctt ccaccaccgc ttcaccaaca
     5821 atatggtgcg cctcgcggaa cggtacgcct ttcgccacca gataatccgc cagttcggtg
     5881 gcgttggcgt aaccctgctg agccgcttcc tggcaacgtg gacgtttcac ctgaatgccg
     5941 tccagcacca gcgccgccat atgcaggcag tccagccagg tatcgagcgc gtcgaacaga
     6001 ccttctttgt cttcctgcat atctttgttg taagccagcg gcaaaccttt cagcgtcatc
     6061 atcatgccgg ttaacgcccc ctgcacccga ccgcatttac cgcgaatcag ctccagcgca
     6121 tccgggtttt tcttctgcgg cattaatgat gaaccggaag tcacgcggtc agaaagctcc
     6181 acaaaccccg cttcgccggt gttaaagaaa atcagatctt cagcaaaacg cgacagatgc
     6241 accatgccga tagcggcagc agaaagcagt tccaacacat ggtcacggtc agaaacgctg
     6301 tcgagactgt tacgggtcgc cgaagcaaag cccagccagc ctgctaactg ttcacggtcg
     6361 atttcatagg ccgttcccgc cagcgcgcca cagcctagcg ggctgacatc cagacgctta
     6421 agcgcatcct gcaaacggct ttcatcacgc gccagcatct caacataggc caggcaccag
     6481 tgcgcgaacg tcaccggctg ggcgcgttgc aggtgagtgt aacctggcat taccgcgtcc
     6541 tgattgtttt gtgcggtttc caccagcgcc gattgcagct gccggttagc cgtcagtaac
     6601 tcgctaacgg tatctttgca ccacagtttc aggtcagtcg ctacctgatc attacggcta
     6661 cgcccggtat gcagcttttt gcctaactgg cccactttgt cgatcagttt gccttccacc
     6721 cagctatgga tatcttcggc gtcgctttca aggatttgtt gtggcctggc gcgaacatct
     6781 tccagcaaca cgttcagcgc ctcttccagt tgcgcctgct cttctgcggt taacacgcct
     6841 accgtgacca gggctttgga ccaggccaca gagccaacaa tatcctgctc cgccagacgg
     6901 taatcaaagc gcagtgagtc gttgaattgt ttgaaccgtt gatctgctgc ctgggtaaaa
     6961 cgcccgcccc aaagtgccat aactctgttt ccttattttg aaattcaatg ccggaaagct
     7021 gcgcctccgg ccaacaaaac ttaagctaaa atccgcgtac ccatcggcat accgttaaac
     7081 agtgccggaa gctgctccgc atgacgccag gaggcgatat ctaccggacg gcccagcgtg
     7141 cgggccgcat ccagcgccgc gttcactttc actatcatgc cgtcagtaat aatgccctgc
     7201 tcaatcagtt gttctgcttt cgcggcggtc atttcggcaa tgcgttgccc tttgccgtcg
     7261 agaatgccgc tgacgtcgga gagcaaaatc agatccgcgc ccagcgttgc cgccagcgcc
     7321 gttgccgcct ggtcggcatt gacgttcatc agttgccctt cgtctgttac gccaatggag
     7381 ctgaccaccg gcagataacc gttctccagc aaggagttga taagcttagg cgaacctggc
     7441 tgcgccagtc caacatgacc taactcttca tcaagctggg tcactttgac gctgtcgccg
     7501 tcaccgagaa acaaacctac ggccgcaatc tgatgtttct tcgcccatgc caacagggtt
     7561 ttatttgccg ttcccgccag tgctccggtg ataatgtcta tctgatcagc aggcgtcacc
     7621 cgcaggccgt ttttcttttt caccggcaga ttcagccctt tcatcagctc atccaccacg
     7681 caaccgccgc cgtgcacaat caccagcgga cgctgatgtg actcacgata attcaccagt
     7741 gcgctaaaca gacgttccag cgcctcttca ctatccagca gtacgccgcc cagtttgata
     7801 attaatggat tcatcattgc acccttaaat aagagactgc gtttccgcat agccgaaacg
     7861 aatattggcg cactgtaccg cttgtgccgc cgcgcctttc agtaagttgt cttcggtcgc
     7921 cacaataatc agatgctcgc cctgaacggc aaacccgata tcgcaaaatg gcagcccaac
     7981 gacatttttc agcgccggaa cgcctttgtc atacagccgc accagcggtt tatgggcata
     8041 cgcctgctgt aacacttgcg cgacttgcgc ctgggtcaca cccgatttca ggcggcaggt
     8101 aatggtttcg agaatgccgc gcgggaaatt gcccagatgt ggggtgaaga taacgtcagc
     8161 accgaggtgt gtggcgatct ctggttgatg gcgatgagta aagacgccat acggttgcag
     8221 gctaacttca caaaagctgt ttgaaatggc cgctttacgc cctgcaccgc tcacgccgct
     8281 ggtggcgttg atcaccggcc actgattgag gtcaagaaga tcggcatcaa tcaacggttt
     8341 cagcgccagc tgtgccgccg tcggataaca gcccggcacc gcaatcaaat tcgcttcttt
     8401 taatttatta ccgcaccact ccgccagacc gtaggctgcc tgttccaaca gttccgggta
     8461 ttgatgggta aagccgtaat atttttcata gaaggtggcg tcgttaacac gaaacgcgcc
     8521 ggaaaggtcg aacaccacgc agcccgcttc aagaaactgc ggcgctaaat cgtggctaac
     8581 ttcatgggcg gtggcgagaa acactacgtc cacccctggg ctaaactcgc tgatatccga
     8641 catcggctgc aacggcagat caacgatgcc ttttagctgc ggatgcaaat cggagattaa
     8701 ctttcccgca tcattgcttt gcgctgaaac agtcaaagcg gttatgttca tatgcggatg
     8761 gcgatttaca taggtcacta gctctgcgcc agcgtagccg ctggcaccca caatcagcgt
     8821 attcaacatc ggggctattc accttcttat gtctggttgc caggttaaac gtaaaacatt
     8881 caccttacgg ctggtgggtt ttattacgct caacgttagt gtatttttat tcataaatac
     8941 tgcatgaata ttgatactat catgaccaga ggtgtgtcaa caatgaaaaa caaattaccg
     9001 ccatttatcg agatttaccg cgctctgatt gccacacctt caataagcgc cacggaagag
     9061 gcactcgatc aaagcaatgc agatttaatc actctgctgg cggactggtt taaagatttg
     9121 ggcttcaatg tggaagtgca gcctgttcca ggaactcgca acaaatttaa tatgctggcc
     9181 agttgcggac agggggctgg cggcttgttg ctggcggggc ataccgatac ggtgccattt
     9241 gatgacggtc gctggacgcg cgatccgttt acactgacgg agcatgacgg caagctttac
     9301 ggcttaggca ccgccgacat gaaaggcttt tttgcgttta tccttgatgc gctacgcgat
     9361 gtcgacgtca cgaaactgaa aaaaccgctc tacattctgg cgactgctga tgaagaaacc
     9421 agtatggccg gagcgcgtta ttttgccgaa actaccgccc tgcgcccgga ttgcgccatc
     9481 attggcgaac cgacgtcact acaaccggta cgcgcacata aaggtcatat ctctaacgcc
     9541 atccgtattc agggccagtc ggggcactcc agcgatccag cacgcggagt taacgctatc
     9601 gaactaatgc acgacgccat cgggcatatt ttgcaattgc gcgataacct gaaagaacgt
     9661 tatcactacg aagcgtttac cgtgccatac cctacgctca acctcgggca tattcacggt
     9721 ggcgacgctt ctaaccgtat ttgcgcttgc tgtgagttgc atatggatat tcgtccgctg
     9781 cctggcatga cactcaatga acttaatggt ttgctcaacg atgcattggc tccggtgagc
     9841 gaacgctggc cgggtcgtct gacggtcgac gagctgcatc cgccgatccc tggctatgaa
     9901 tgcccaccga atcatcaact ggttgaagta gttgagaaat tgctcggagc aaagaccgaa
     9961 gtggtgaact actgtaccga agcgccgttt attcaaacgt tatgcccgac gctggtgttg
    10021 gggcctggct caattaatca ggctcatcaa cctgatgaat atctggaaac acggtttatc
    10081 aagcccaccc gcgaactgat aacccaggta attcaccatt tttgctggca ttaaaacgta
    10141 ggccggataa ggcgctcgcg ccgcatccgg cactgttgtc aaactccagt gccgcaataa
    10201 tgtcggatgc gatacttgcg catcttatcc gacctacacc tttggtgtta cttggggcga
    10261 ttttttaaca tttccataag ttacgcttat ttaaagcgtc gtgaatttaa tgacgtaaat
    10321 tcctgctatt tattcgtttg ctgaagcgat ttcgcagcat ttgacgtcac cgcttttacg
    10381 tggctttata aaagacgacg aaaagcaaag cccgagcata ttcgcgccaa tgcgacgtga
    10441 aggatacagg gctatcaaac gataagatgg ggtgtctggg gtaatatgaa cgaacaatat
    10501 tccgcattgc gtagtaatgt cagtatgctc ggcaaagtgc tgggagaaac catcaaggat
    10561 gcgttgggag aacacattct tgaacgcgta gaaactatcc gtaagttgtc caaatcttca
    10621 cgcgctggca atgatgctaa ccgccaggag ttactcacca ccttacaaaa tttgtcgaac
    10681 gacgagctgc tgcccgttgc gcgtgcgttt agtcagttcc tgaacctggc caacaccgcc
    10741 gagcaatacc acagcatttc gccgaaaggc gaagctgcca gcaacccgga agtgatcgcc
    10801 cgcaccctgc gtaaactgaa aaaccagccg gaactgagcg aagacaccat caaaaaagca
    10861 gtggaatcgc tgtcgctgga actggtcctc acggctcacc ctaccgaaat tacccgtcgt
    10921 acactgatcc acaaaatggt ggaagtgaac gcctgcttaa aacagctcga taacaaagat
    10981 atcgctgact acgaacacaa ccagctgatg cgtcgcctgc gccagttaat cgcccagtca
    11041 tggcataccg atgaaatccg taagctgcgt ccaagcccgg tagatgaagc caaatggggc
    11101 tttgccgtag tggaaaacag cctgtggcaa ggcgtaccaa attacctgcg cgaactgaac
    11161 gaacaactgg aagagaacct cggctacaaa ctgcccgtcg aatttgttcc ggtccgtttt
    11221 acttcgtgga tgggcggcga ccgcgacggc aacccgaacg tcactgccga tatcacccgc
    11281 cacgtcctgc tactcagccg ctggaaagcc accgatttgt tcctgaaaga tattcaggtg
    11341 ctggtttctg aactgtcgat ggttgaagcg acccctgaac tgctggcgct ggttggcgaa
    11401 gaaggtgccg cagaaccgta tcgctatctg atgaaaaacc tgcgttctcg cctgatggcg
    11461 acacaggcat ggctggaagc gcgcctgaaa ggcgaagaac tgccaaaacc agaagggctg
    11521 ctgacacaaa acgaagaact gtgggaaccg ctctacgctt gctaccagtc acttcaggcg
    11581 tgtggcatgg gtattatcgc caacggcgat ctgctcgaca ccctgcgccg cgtgaaatgt
    11641 ttcggcgtac cgctggtccg tattgatatc cgtcaggaga gcacccgcca taccgaagcg
    11701 ctgggcgagc tgacccgcta cctcggtatc ggcgactacg aaagctggtc agaggccgac
    11761 aaacaggcgt tcctgatccg cgaactgaac tccaaacgtc cgcttctgcc gcgcaactgg
    11821 caaccaagcg ccgaaacgcg cgaagtgctc gatacctgcc aggtgattgc cgaagcgccg
    11881 caaggctcca ttgccgccta cgtgatctcg atggcgaaaa cgccgtccga cgtactggct
    11941 gtccacctgc tgctgaaaga agcgggtatc gggtttgcga tgccggttgc tccgctgttt
    12001 gaaaccctcg atgatctgaa caacgccaac gatgtcatga cccagctgct caatattgac
    12061 tggtatcgtg gcctgattca gggcaaacag atggtgatga ttggctattc cgactcagca
    12121 aaagatgcgg gagtgatggc agcttcctgg gcgcaatatc aggcacagga tgcattaatc
    12181 aaaacctgcg aaaaagcggg tattgagctg acgttgttcc acggtcgcgg cggttccatt
    12241 ggtcgcggcg gcgcacctgc tcatgcggcg ctgctgtcac aaccgccagg aagcctgaaa
    12301 ggtggcctgc gcgtgactga acagggcgag atgatccgct ttaaatatgg tctgccagaa
    12361 atcaccgtca gcagcctgtc gctgtacacc ggggcgattc tggaagccaa cctgctgcca
    12421 ccacctgagc cgaaagagag ctggcgtcgc attatggatg aactgtcagt catctcctgc
    12481 gatctctacc gtggctacgt acgtgaaaac aaagattttg tgccttactt ccgctccgct
    12541 acgccggaac aagaattggg caaactgccg ttgggttcac gtccggcgaa acgtcgccca
    12601 accggcggcg tcgagtcgct gcgcgccata ccgtggattt ttgcctggac gcaaaaccgc
    12661 ctgatgctcc ccgcctggct gggtgcaggt acggcgctgc aaaaagtggt cgaagatggc
    12721 aaacagagcg agctggaagc catgtgccgc gattggccat tcttctcgac gcgtctcggc
    12781 atgctggaga tggtcttcgc caaagcagac ctgtggctgg cggaatacta tgaccaacgc
    12841 ctggtagaca aagcactgtg gccgttaggt aaagagttac gcaacctgca agaagaagac
    12901 atcaaagtgg tgctggcgat tgccaacgat tcccatctaa tggccgatct gccgtggatt
    12961 gcagagtcta ttcagctacg gaatatttac accgacccgc tgaacgtatt gcaggccgag
    13021 ttactgcacc gctcccgcca ggcagaaaaa gaaggccagg aaccggatcc tcgcgtcgaa
    13081 caagcgttaa tggtcactat tgccgggatt gcggcaggta tgcgtaatac cggctaatct
    13141 tcctcttctg caaaccctcg tgcttttgcg cgagggtttt ctgaaatact tctgttctaa
    13201 caccctcgtt ttcaatatat ttctgtctgc attttattca aattctgaat ataccttcag
    13261 atatccttaa ggaattgtcg ttacattcgg cgatattttt ttcaagacag gttcttacta
    13321 tgcattccac agaagtccag gctaaacccc tttttagctg gaaagccctg ggttgggcac
    13381 tgctctactt ttggtttttc tctactctgc tacaggccat tatttacatc agtggttata
    13441 gtggcaccaa cggcattcgc gactcgctgt tattcagctc gctgtggttg atcccggtat
    13501 tcctctttcc gaagcggatt aaaattattg ccgcagtaat cggcgtggtg ctatgggcgg
    13561 cctctctggc ggcgctatgc tactacgtca tctacgggca ggagttctcg cagagcgttc
    13621 tgtttgtgat gttcgaaacc aacaccaacg aagccagcga gtatttaagc cagtatttca
    13681 gcctgaaaat tgtgcttatc gcgctggcct atacggcggt ggcagttctg ctgtggacac
    13741 gcctgcgccc ggtctatatt ccaaagccgt ggcgttatgt tgtctctttt gccctgcttt
    13801 atggcttgat tctgcatccg atcgccatga atacgtttat caaaaacaag ccgtttgaga
    13861 aaacgttgga taacctggcc tcgcgtatgg agcctgccgc accgtggcaa ttcctgaccg
    13921 gctattatca gtatcgtcag caactaaact cgctaacaaa gttactgaat gaaaataatg
    13981 ccttgccgcc actggctaat ttcaaagatg aatcgggtaa cgaaccgcgc actttagtgc
    14041 tggtgattgg cgagtcgacc cagcgcggac gcatgagtct gtacggttat ccgcgtgaaa
    14101 ccacgccgga gctggatgcg ctgcataaaa ctgatccgaa tctgaccgtg tttaataacg
    14161 tagttacgtc tcgtccgtac accattgaaa tcctgcaaca ggcgctgacc tttgccaatg
    14221 aaaagaaccc ggatctgtat ctgacgcagc cgtcgctgat gaacatgatg aaacaggcag
    14281 gttataaaac cttctggatc accaaccagc agacgatgac cgcccgcaat accatgctga
    14341 cggtattttc gaagcagacc gacaaacagt tctatatgaa ccagcaacgt acgcagagtg
    14401 cgcgtgaata cgacaccaac gtgctgaaac cattccagga agtgctgaag gacccggcgc
    14461 cgaagaaact gatcatcgtt catctgctgg gtacgcatat caaatacaaa taccgctacc
    14521 cggaaaatca gggcaagttt gatggcaata ccgatcatgt tccgcctgga ttaaacgcgg
    14581 aagagctgga gtcatataac gattatgaca acgctaacct gtataacgat catgtggttg
    14641 ccagcctgat taaagacttt aaagcagcaa acccgaacgg tttcctggtt tatttctctg
    14701 accacggtga agaggtttac gacacgccgc cgcacaaaac tcaggggcgt aacgaagaca
    14761 acccgacgcg ccacatgtac accattccgt tcctgctgtg gacgtcggaa aaatggcaag
    14821 cgactcatcc gcgtgatttc tcgcaggatg ttgatcgtaa atacagcctg gcggaactga
    14881 tccacacctg gtcagattta gcaggcttat cttacgacgg ttacgatcca acccgttcag
    14941 tggtgaatcc gcagttcaaa gaaactaccc gctggattgg taacccgtac aagaaaaacg
    15001 cgctgatcga ttacgacacg ctgccgtatg gcgatcaggt gggtaatcag taaatgacct
    15061 ttgtatcgcg tctggcgcta tttgccggat gcgatactgc cgcaatgttt acatcgaact
    15121 cccctcaggc cacacttttc aggtggcctt ttttaataat gccagttact gttttcccgt
    15181 ctacatacct aagggaataa catgtatcac gacgtcagct atctgctctc ccgcctgatc
    15241 aacggcccgc tgtccctgcg ccagatttac tttgccagca gtaacggccc ggtgcccgat
    15301 ctcgcgtatc aggtcgattt tccacggctg gaaattgtgc tggaaggtga gtttgttgat
    15361 accggcgctg gagcaacgtt agttcccggc gatgtgctgt acgttcctgc tggtggctgg
    15421 aattttccac aatggcaagc ccccgctact acctttagcg tgctgtttgg caaacagcaa
    15481 ctcggcttca gcgtcgtgca atgggatggc aaacaatatc aaaatctggc gaagcaacac
    15541 gtcgcccggc gcggcccacg cataggttct tttctgctac aaacgctcaa tgaaatgcaa
    15601 atgcagccgc aggagcagca aacggcaagg cttatcgtcg ccagcctgct tagccactgc
    15661 cgcgatttgc tcggcagcca aatccagacc gcctcacgca gccaggcact atttgaagct
    15721 attcgcgatt atatcgacga acgctatgcc tccgcgctta cccgcgaatc tgtcgcacag
    15781 gcattctata tttcaccaaa ctatctctcg cacctgtttc aaaaaacggg ggccattggt
    15841 tttaacgaat acctgaatca cacgcgactg gagcacgcta agacgttatt gaaaggctat
    15901 gacctgaagg taaaagaagt ggcgcacgcc tgcggctttg tcgacagcaa ctacttctgc
    15961 cgtctgtttc gtaaaaacac cgaacgctcg ccgtcggaat atcgtcggca gtaccacagc
    16021 caactgacag aaaaaccgac tactccagaa taagatgggt ttgctgcggc gcagaaagca
    16081 ctttgcgcac cgcgctcatt acccgctgcg gctcacgcag gaatgcgtag atgctgcatt
    16141 gcacatagcg gcaatgttca aatcgctcgg caccggcaag ctcgatatcc gtaatcaaca
    16201 gagcaacatc cgcccgacgg atatcctcgt ccgctaaacg attctccgtt cccagcgcgc
    16261 cctgagtttc aatgctgacg ccccacttcc ctaactggca caacttttcc agccgttccg
    16321 ccgccatata agtatgcgcc acgccgctta cgcaggcggt tactgccacc aggtatgcca
    16381 ttttaacctc ccacggtaac ctgaaatccg gcccgttctg ccatttcgcg catcgttgtc
    16441 acatcggctg acgacggcgc aggcacctct ttcatcgacc atgttttccc cagcaggcgg
    16501 tatttcggtt cgccgtactg atgaaacggt aacagatgga tctgcctgat tttcagcggg
    16561 atcagtacat ccagcgcctg ctgcatattc tcccggctga gcgtgaaacc agggatcaac
    16621 ggtaaacgcg ggatcacgtt gacaccctca ctcaccagca aacgcagatt ctccagcacg
    16681 cgtggcaggt tcatcttcac cacatcccgc gcctgagtcg cgtccataat ttttaaatcg
    16741 aacagcactt catcgcacaa tttcgccagt ggtaacagct tgctggctgg cgcgtctccg
    16801 gcagtttcaa tggcgcatga cacgccccac aaccgcagcc gctgtaaaaa acgggtagca
    16861 aactccgcct gcattaacac ttcgccgcca gaaagcgtga cgccgccgcc ggacgtgcga
    16921 aagaaaatgt catctttcat cacttcccgt tccagcgcgt caaggctgat atcgcgacca
    16981 atccgttcaa acgccccgga ggggcattca tccgcatcac gcaaacattt cgcacagtgc
    17041 agacatttcg cctctctgcg taccgtctgg attttgccgg agatcgactc cggattagca
    17101 caccatgggc aaagatgcgg acagccttta aaaaagacca ccgtacgaat gccctcgccg
    17161 tcattcagtg aataacgctg aatgttgaaa atgcgcgcca catcattgcg gcgcgtttcc
    17221 acaacgttac agctgatgcg ctgtccggcg gatgatgtca tcctggatct ccttcgacag
    17281 ttcgacaaag aaggcgctgt atccggcaac gcgcaccacc agcccggcat aatcttgtgg
    17341 gcgctgttgc gcttcccgca acgtgtcggc gttcaccacg ttaaactgaa tatgctgcaa
    17401 cttaagctgg gtaaacgccc gtaagaagtc ggccagtttg cgtaatcccg cttcaccttc
    17461 cagggtcgcc ggagtgaatt tcacgttcag caacgtaccg ttagacagca gcgtattatc
    17521 gagcttactg actgacttca gtaccgctgt tggcccttgt gcgtcctggc ccagcatagg
    17581 tgacaagccg ccgtctgcca gttgttcccc ggcaaaacga ccgtctggcg tcgcgccaac
    17641 caccgatccc aacgggacgt gagcagaaac ggtatacgat cccggcgtga agtaaccacc
    17701 gcgcgggttc tggtattttt ccacttcttt gcagtagtgg cgtaacagtt cggcgctgat
    17761 gttatccacc tcgtcgatat cgttaccgta tttctcgaag cggttaatta agcgagcacg
    17821 gactttttcg ccttctggcg ttgcaaagtt ggcttttaat accgacagca attcgtcaaa
    17881 acttaaacgc tgttgctcaa aaaccatccc tttcaacgca tggagagaat cgctcaggtt
    17941 ggcgataccg atcccctgta cgccggagaa gttataacgc gtgccgccat cggtaatatc
    18001 gcgacctttt tccagacaat cgctgataaa agacgagagc agcggtacag gtgcccagtc
    18061 gcgatggccg atatcacaaa tattgctgcc ctcaaccatc agggtgatgt agtggctgat
    18121 cttggcgcgg atctgctcca gtaaaccttc ataagtcagc gcagcattgc cttcattttc
    18181 atgcaggcag atttccatca ctttcagcag attaaacatc gcgatgtcat gcaagccgta
    18241 ggttctgccg ggaatagata attccacaca gcccactacg gaatagtcgc gcgcatcttc
    18301 cagcgaaacg ccacggttga ggaacgctgg taccaccact tcatcgttaa agatttgcgg
    18361 aataccggtg ccgaggcgaa tggtttcggc ggttttcatc aggaacggcg tgtcgatcaa
    18421 ggcgttagtg cgcacgccga ggttcggttg cggtaattgc acgctttgat aagcgtcaag
    18481 gcaaaggaac gaaagcacgt tcaccgcgct acgtccgttc tcggttaacc cgccgagcag
    18541 tgcggtatag ccggtcggga aacctgcgaa ataacgcgcg ctgctggtgg agcgcaacag
    18601 cacgatgtcg ttgcatttca cccataaaga ttcgagtagt tctttcagga acgccggatc
    18661 ttcgccctgg gttaaagatg cctgatagaa cggcaacata tactggtcga agcgccccaa
    18721 cgatagcgaa ctggcgttgg attcgtattg cagaatgatg ttcatgtacc agaataactg
    18781 gcacgcctgc cagaacgtct gcggcttatg ttcggcgtta tggcgggaga tctccgcaat
    18841 agtcagcagt tcttcgcgac gctgggcatc tgtgcagttt gccgccattg tttccgccag
    18901 ttcagcgtaa cgcagaatgt gtttctgcga ggcttccagc agtaacagcg cggcctgata
    18961 aaagtgattc tccggctgtt gctgacaatg ttgctgcatc tgcgccacca gctcccccag
    19021 cccgtgattc agcaggcgtg ggtaatcaat aataatgtgc ccctggcctt tatctgtctg
    19081 gttgatgctg aaaatctgcg tgctggtcgc ggcttttact tcatctgtca tctgcccgtt
    19141 gatgaaatct ttcatcgaac gtttttccca gtacgggaac aactcttcac ggtagatacg
    19201 tttatcttct tcactgatag caaagcggtc ctgcggacgc gtcgggaatt gatccagctc
    19261 tttcagcagc cagtaagggt ccatttccgg cgacataatc ccagcgcgcg gttttacggt
    19321 gcggttaccg gcaatcagtt cttcatcacg aatcgaaatt tcaacatgtt caaggatata
    19381 cgctgttgct ttcgcccggc gcaatatcac cggttcgcct tcggtttgcc gatggctggc
    19441 ggtataaagc agcgcccgct ccagcgagat ttcacgggta ttggcaaaca gtgcagtttt
    19501 gaggcgagag atacgattcg tcatgaggaa ctcctgtggg aaagcgaacc cgcccttgcg
    19561 agcgggagaa gaacttaagc ggtttgcgcc aggtgagctt caattttgct cataatggcg
    19621 tcagcacgtt ttactgcatc gctgatgttg acgcgcacga tggtcttgcc agcaaaacgc
    19681 tcttcaaact tgatgccgat atctttggtc agaatcacca tatcagcgct cgccacatct
    19741 tccgcagtca gttcattttc cagaccaatt gacccctggg tttctacttt cacttcccag
    19801 cctttcgctt tcgcggcact ttccagcgcc tctgctgcca tataggtatg ggcaacacct
    19861 gaaggacatg cggttactgc aataattttc gtcatggctc gttcctcaat taattaattt
    19921 caaaatccaa atccaggtcg tcttcttttt catcagtcga agacccattt ttacgcgcca
    19981 gacttttcag cacgttcaca caaacagccg tcaccactgc tccgacagcc actgctgcga
    20041 tataacccag cttgccttca accaccggca gcacaatcag accaccccag cctgcatagc
    20101 actgcgcacc gaccagcgcc gcagttactg caccacaaac tgaaccgacc atgatggaag
    20161 gaatgacacg cagcggatcg gcagcggcga aaggaatcgc cccttcagta acgccaacac
    20221 agcccatcac cagtgccgct ttgccggttt cgcgctcttc tgcggagaaa tttttacgac
    20281 taatcagcgt cgccagcccc atccccagcg gcgggataca aatacccacg gcagcgatgg
    20341 ccaccacggt ataaacaccc tgagcaacgc aaatcagcat gaaggcatag gccactttgt
    20401 taaccggacc gcccatatcg aacgccagca tcagacccat gatcaccgcc agcataacaa
    20461 tgctgccctg ctgcatcccc tgaagccact gagtcaggct gttggtcaac gcccctacag
    20521 gctcgcccaa gccccacatc atgatgcctg cggtaatcag tgtgccgacg ataggaataa
    20581 tgaagatagg catcaccgag cgcagaactt tatgcaccgg aattttcttc aggtaatgca
    20641 ccacgatgcc gccgataatc ccggcgatca gtgcaccaaa gaagcccgca ccaaagctgt
    20701 taccaaccca ggcaccgata gcgcacggag ccagcgcaga acgctctgca atggaataac
    20761 cgatgtacgc tgcgaggaaa ggcaccatca gcgtcaagcc cgcaacgccg atatcaaaca
    20821 gttttttcag atttggatcg gctacggcat ccggcactgc gcctttgcca tacaacatga
    20881 cggaaaccgc cagcaaaata ccgcccgata ccacgaaggg aatcatgtgt gaaacgcccg
    20941 tcattaaatg ctgacgggta tttttcagga tctgcaccaa ctcattcata agctgctccc
    21001 gggcttgcga aagcccatgt ccatattttt gatggaggaa acaataggca atcgcaggcg
    21061 gagcaaacag tggataaaaa ggccatttac tggacttttg taatatcagt acaaaaatgc
    21121 gatccgcctc ataacttgcg ataaaccgga agcatgccgc aaaacagcgt cttgtgagac
    21181 gcgtcgcaaa ttgacggtta cattacattt gtccagcttt tggcagattt gtcacatggc
    21241 aggccgttgc cgtgcgctct tatcctgtaa acagaaaacc gtaccgggag agaggcaatg
    21301 gccctgattg tggaatttat ttgtgagcta cctaacggcg tacatgcacg tccggcaagc
    21361 cacgttgaaa cgctgtgtaa tactttttca tcacaaattg agtggcataa cctgcgcact
    21421 gaccgcaagg gcaacgccaa aagcgccctt gcgctgattg gcaccgatac gctggcgggc
    21481 gataactgcc agttactgat ttccggggcc gacgaacagg aagcgcacca gcgtttaagc
    21541 caatggctgc gcgatgaatt cccccactgc gatgcgccgc tggcggaagt taaatctgac
    21601 gaactggaac cactgccggt ttcactgacc aatctgaatc cgcaaattat ccgcgcccgc
    21661 accgtgtgca gcggtagtgc aggcggcatt ctgacgccga tctcttcttt agatctcaat
    21721 gcgctgggta atcttcccgc agccaaaggc gttgacgccg agcaatccgc actggaaaac
    21781 ggcctgacgc tggtactgaa aaacattgag tttcgtctgc tggatagcga cggtgccacc
    21841 agcgcgattc tggaagcgca ccgatccctg gctggcgata cttccctgcg cgaacattta
    21901 ctggcaggcg tcagcgccgg attaagctgc gccgaagcga ttgtcaccag tgcgaatcac
    21961 ttttgcgaag agttcgcccg ttccagcagc agctacctgc aagaacgtgc cctggacgta
    22021 cgcgacgtct gcttccagtt actccagcaa atctacggtg agcaacgctt cccggcaccg
    22081 ggcaaactga cgcagcccgc catttgtatg gctgatgaac tgactcccag ccagttcctc
    22141 gaactggata aaaatcacct caaaggtctg ttactgaaaa gtggcggcac cacctcacat
    22201 accgtaattc ttgcccgttc gttcaacatt ccgacactgg ttggtgtaga tattgatgcc
    22261 cttactccgt ggcagcatca aacgatttat atcgacggca acgctggggc gattgtggtt
    22321 gagccaggag aagccgtagc tcgttattat cagcaagaag cccgcgtaca ggacgccctg
    22381 cgtgagcaac agcgtgtctg gctgacccaa caagcccgta ccgctgacgg tatccgcatt
    22441 gaaattgccg ctaacatcgc tcactccgtg gaagcgcagg ccgcattcgg caatggtgcg
    22501 gaaggcgttg gtttgttccg cactgaaatg ctctatatgg atcgcaccag tgcaccgggc
    22561 gaaagcgagc tgtacaacat tttttgtcag gcgctggagt ctgccaacgg acgcagcatt
    22621 attgtgcgca ctatggacat tggcggcgac aaacccgttg attatctgaa cattccggca
    22681 gaagctaacc cgttcctcgg ttatcgcgct gtgcgtattt atgaagaata cgcctcgctg
    22741 ttcaccacac aactacggtc gatcctgcgc gcctccgctc acggcagcct gaaaatcatg
    22801 atcccgatga tctcctcaat ggaagagatc ttatgggtga aagaaaaact ggcggaagcc
    22861 aaacagcaac tgcgtaacga acacattccg tttgatgaga agatccagct tggcattatg
    22921 ctggaagtac cgtcggtgat gttcatcatc gatcaatgct gcgaagagat tgatttcttt
    22981 agtattggta gtaatgacct gacgcaatat ctgctggcag tggatcgcga taacgctaag
    23041 gttactcgtc actacaacag cctgaatccg gcattcttgc gggcgctcga ttacgccgtg
    23101 caggcggtgc atcgccaggg caaatggatt ggtctgtgcg gtgagctggg agcgaaaggt
    23161 tccgtgctgc cgttgctggt cggcttaggg ctggatgaac tcagcatgag cgcaccatca
    23221 attccggcgg cgaaagcgcg gatggcgcaa cttgatagcc gtgagtgccg caagttgctc
    23281 aaccaggcaa tggcctgccg tacttcgctg gaagtggaac acctgctggc gcaattccgc
    23341 atgacccaac aggacgcacc gctggtcacc gccgagtgca tcacgctgga aagtgactgg
    23401 cgcagcaaag aagaagtgct caaaggcatg accgataacc tgctgctggc gggccgctgc
    23461 cgctatccgc gtaaactgga agccgactta tgggcgcgcg aggccgtttt ctctaccggt
    23521 ctgggcttta gttttgccat tccacacagc aaatcagaac acattgagca atccaccatc
    23581 agcgtggcgc gtctgcaagc gccggtgcgc tggggcgatg atgaagcgca attcatcatt
    23641 atgttaaccc tgaacaaaca cgctgcgggc gatcagcaca tgcgcatttt ctcgcgcctc
    23701 gctcgccgca tcatgcacga agaattccgt aacgcgctgg ttaacgccgc ctctgccgac
    23761 gctatcgcca gcctgctgca acatgaactg gaactgtaaa aggaaacatc atggaactgt
    23821 atctggacac cgctaacgtc gcagaagtcg aacgtctggc acgcatattc cccattgccg
    23881 gggtgacaac taacccgagc attatcgctg ccagcaagga gtccatatgg gaagttctgc
    23941 cgcgtctgca aaaagcgatt ggtgatgagg gcattctgtt tgctcagact atgagtcgtg
    24001 acgcgcaggg tatggtggaa gaagcgaagc gcctgcgcga cgccattccg ggcattgtgg
    24061 tgaaaatccc ggtgacttcc gaaggtctgg ccgccattaa gatgctgaaa aaagagggta
    24121 ttactacgct tggcaccgca gtgtacagtg ctgcacaagg gttactggcc gcgctggcag
    24181 gggcaaaata cgttgctccg tatgttaacc gcgtagatgc ccagggcggg gacggcattc
    24241 gtacggttca ggagctgcaa gcgctgttag aaatgcacgc accagaaagc atggtgctgg
    24301 cggccagctt taaaacgccg cgtcaggcgc tggactgttt actggcagga tgtgaatcca
    24361 tcaccctgcc cttagatgta gcgcaacaaa tgctcaacac ccctgcggta gagtcagcta
    24421 tagagaagtt cgaacacgac tggaatgccg catttggcac tactcatctc taaaggagca
    24481 attatggacc gcattattca atcaccgggt aaatacatcc agggcgctga tgtgattaat
    24541 cgtctgggcg aatacctgaa gccgctggca gaacgctggt tagtggtggg tgacaaattt
    24601 gttttaggtt ttgctcaatc cactgtcgag aaaagcttta aagatgctgg actggtagta
    24661 gaaattgcgc cgtttggcgg tgaatgttcg caaaatgaga tcgaccgtct gcgtggcatc
    24721 gcggagactg cgcagtgtgg cgcaattctc ggtatcggtg gcggaaaaac cctcgatact
    24781 gccaaagcac tggcacattt catgggtgtt ccggtagcga tcgcaccgac tatcgcctct
    24841 accgatgcac cgtgcagcgc attgtctgtt atctacaccg atgagggtga gtttgaccgc
    24901 tatctgctgt tgccaaataa cccgaatatg gtcattgtcg acaccaaaat cgtcgcgggc
    24961 gcacctgcac gtctgttagc agcgggtatc ggcgatgcgc tggcaacctg gtttgaagcg
    25021 cgtgcttgct ctcgtagcgg cgcgaccacc atggcgggcg gcaagtgcac ccaggcggcg
    25081 ctggcactgg ctgaactgtg ctacaacacc ctgctggaag aaggcgaaaa agcgatgctt
    25141 gccgccgaac agcatgtagt aaccccggcg ctggagcgcg tgattgaagc gaacacctat
    25201 ttgagcggcg ttggttttga aagcggcggg ctggcggcgg ctcacgcggt gcataacggc
    25261 ctgaccgcta tcccggacgc gcatcactat tatcacggtg aaaaagtggc atttggtacg
    25321 ctgacgcagc tggttctgga aaacgcaccg gttgaggaaa tcgaaaccgt agctgcactt
    25381 agccatgcgg taggtttgcc aataactctc gctcaactgg atattaaaga agatgtcccg
    25441 gcgaaaatgc gaattgtggc agaagcggca tgtgcagaag gtgaaaccat tcacaacatg
    25501 cctggcggcg cgacgccaga tcaggtttac gccgctctgc tggtagccga ccagtacggt
    25561 cagcgtttcc tgcaagagtg ggaataacct actccaaact cccggcttgt ccgggagttt
    25621 gaacgcaaaa ttgcctgatg cgctacgttt atcaagccta cgaaatctct gcaatatatt
    25681 gaatatgcgt gcttttgtag gccggataag gcgttcactc cgcatccggc actctgtgcc
    25741 agtaatctga aactcccacg ttgttcagga gttttttatc cgcttctgga cattttctcc
    25801 acagaacaat cgttagcccg gaaggtcgaa tctgtgactc ttattttcac ggacgaacca
    25861 gatgaaagct tcactggcgc tgctcagcct gctaacggca tttaccagcc actccttaaa
    25921 atctcccgcc gttccaccaa ccgtggtaca aatccaggcc aataccaacc ttgccatcgc
    25981 agacggtgcc agacagcaaa tcggcagcac gctattttac gatccggcgt atatgcagct
    26041 tacctatcct ggcggtgatg tcccgcaaga acgcggggta tgttccgatg tggtgatccg
    26101 cgccctgcgc agccagaagg tcgatttgca gaaactggtt cacgaagata tggcgaagaa
    26161 ttttgccgag tacccgcaaa aatggaagtt aaagcgcccg gacagcaaca tcgatcaccg
    26221 tcgggtgcct aatctggaaa cctggtttag ccgccacaac aaaacacgcc ccatcagtaa
    26281 aaatgccggt gactatcaag cgggcgatat tgtctcctgg cgattagata acgggctggc
    26341 gcatattggg gtggtgtcag atggcttcgc ccgcgacggt acgccgctgg tgatacataa
    26401 cattggcgca ggcgcgcagg aagaagatgt actgtttagc tggcggatgg tggggcatta
    26461 tcggtatttt gttaaataag cgatcgcccc acatgaagaa aaaattcaga tgctttttac
    26521 actgacagct tcttttttct tacggctcac cagcgccagt gccagcacaa tcagcacaat
    26581 accgctacct tcaaccacgc caggattctc gccgagcagc caccaggaaa agagtacgcc
    26641 gcaaacggga acggccagtg tgcttaagct ggcaatactg gcaggcaagt ttttcaatac
    26701 aaacaaccat aagctccacg ccagcgccgt cgccagaatc gcactgtaga ccagcgccca
    26761 gaacacagtg ggctgccagt caatttcacg ttgtggcacc agtaaagcga ccacactcat
    26821 caccagcgcc gcatacagca tctgccagga tgttaacgac aataaatcca cgcgcggatg
    26881 acgggcatac agacgtttag caacaatcgc gctcgccccc caactaacac cggagagaat
    26941 tgccagcatg gcacttttca tcgaagagaa atcgagttgc cacggctgca acaccaaaaa
    27001 taagccgaaa gcggcaatca gtatcgcgaa atattgccca cgtcgcaggc gttcaccgag
    27061 aaacaacgcg gcgaaaatca ccacccagaa cggcatggta tagctcagga tcgccacctt
    27121 ccccgcacct ccgctgacca acgcccattg tgccaggcca atcattccgc aggtttgtaa
    27181 cagggcaata gctaaggtgt atttaaacgg cgtcgggcgc attgcgcgac cacgtaataa
    27241 aaggacgatg aataaaacga gagcgccgaa aatgcagcgt aaggcggtga agtcgaaggc
    27301 accgatgtaa ctggtgactt gcttcatgaa aatccagcta taactccaga taagtgtgag
    27361 cacaaccagg ccactgattg ccagtgggtt gctctttcct gcggcagaca tgagagatcc
    27421 agtgtgtagt tttcctgtgg acactatacg ccggtaaaga aacttcagcg actgcctgaa
    27481 agacagtcgc tgaagaaggt cagattacag caggtcgaaa cggtcgaggt tcatcacttt
    27541 cacccatgcc gccacgaagt ctttaacaaa cttctcgtgg gcatcgctgc tggcgtaaac
    27601 ttccgctacc gcacgcagga cagagttaga accaaatacc agatccgcac ggctggctgt
    27661 gtatttcact tcgccggttt cacggtcacg accttcgaac agctctttcg attcgtcagt
    27721 cgctttccac tcgtaacgca tatccagcaa gttcacgaag aagtcattgc tcaatacgcc
    27781 aacgcggtca gtgaagacgc cgtttttgct gccatcgaag ttggcaccca gtacacgcat
    27841 cccgcccacc agcgcagtca tttccggcgc ggtcagcgtc agttgctgtg ctttgtcgat
    27901 cagcagtgac tcggtggtgg aaacgtccag acgagcgcga tagttacgga aaccgtcagc
    27961 aattggctcc agcagctcaa acatctcaat gtcagtctga tcctgacgcg catcaacgcg
    28021 acccggcgca aacggtacat gaatgctcaa acctgcggcg cttgcggctt tctcaacacc
    28081 aaccacacca gccagcacga tgatatccgc cagcgaggct ttaccagact ctttctggat
    28141 tttctccaga acaggcagag cacgaacggc tgcggcgttc acatcccagt cgcgctgcgg
    28201 cattaatgcc agacgcgcac cgttggcacc accgcgtttg tcgccaccac ggaaggtaga
    28261 agcagatgcc caggccaccg ataccagctc actaacagac agaccagaat ccgcaatcgc
    28321 gaatttcaga tcgataatgt cctgctcggt cgggttgtag atcggctgcg gcagcggatc
    28381 ttgccagatc agatcttctt tcggtacttc cggtccgatg taacgagatt tcggccccat
    28441 atccctgtgc gtcagtttga accaggcacg ggcaaaggct tcgttgaacg cctgcggatc
    28501 gttgaggaaa cgacgagaga tcttctcgaa ctcaggatca aaacgcagcg tcaggtcggt
    28561 caccagcatc gtcggtttac gtttcttcga cggatcgaac ggatccggga taatttccgg
    28621 tgcgtctacc gcttcgaact ggattgcgcc agccggactg cgggtctgta cccactcata
    28681 tttgaacagg ttctcgaaga aatagttgct ccactgggtc ggcgtctggg tccagacaac
    28741 ttccagacca gaggtaatgg catctgcgcc aacgccgctg ccgtaagtgc tcgcccagcc
    28801 taaaccttgt tcttcaatcg gtgcagcttc tggatcagga cctacatttg atgtcggacc
    28861 ggcaccgtgg gttttaccca gcgtatgacc acccgcaatc agcgccacgg tttcttcgtc
    28921 gttcatgccc atgttgccga aggtcgcgcg gatagctgct gccgcagaaa gcggttcgcc
    28981 gctgtggtcc gggccttccg ggttaacgta aatcaggccc atctcggttg cacccagcgg
    29041 tgctttcgcc agcgcttccg gatgacggtg agtcagccag gctttttcat caccccagtt
    29101 aacatccaga tccggttccc agacgtcttc acgaccggca ccaaaaccga aggtacggaa
    29161 gccggagttt tccagcgcca cgttacctgc gaggataaac aggtcggccc aggagatttt
    29221 ctgaccatat ttctgtttga ttggccacaa caggcgacgc gctttatcga ggcttacgtt
    29281 atccggccag gagttcagcg gtgcaaaacg ttgctgacca cgacccgcgc cgccgcgtcc
    29341 atcgattgaa cggtaagtac ccgcgccgtg ccaggccata cgaataaaca gaccggcgta
    29401 actgccccag tcggcaggcc accacggctg agattctgtc agcagggctt tcagatcttt
    29461 tttcaggccg tagtaatcta atttgctgaa ttctttgcgg tagtcaaagt cctcacccag
    29521 tgggttagaa cgattagaat gttggtttaa caggtcaaca cggagttgat ttggccacca
    29581 gtcgcgagtg gttgtgcccg cccccgcgct ctggtcgtga ccgccctgat ggaacgggca
    29641 tttgccagtg gctgtggtgt tatgaatatc gtctgacgtg ctcatcaatg tgctcccctc
    29701 tacagtgtta ccgttacgat acacagcgtt agagagaagt tataattgaa ttaatccacg
    29761 gatgcgatag ttgagatctt acatattgtt ggttaaagag atgtagatca aattgatcgt
    29821 aattaagctt ttttgattat gtccgaattc ggacaaatgc tttataaaaa agggttttag
    29881 gttcatatcc ttcaagtaat gagggagaaa acaaggctga taaccttatt ttcccgccct
    29941 catttcgagg cagcattttg tgctctgttt aaaatttgtg atcactgtgt gattttcaca
    30001 aaagccacac tatttataaa ccaggtcgaa cacccagcgt atggcaaatc gcgtaactca
    30061 tttcagcgcg gttaagcgta tagaagtgga aatctttcac tccttcacgg cttaaaatct
    30121 tcaccatatc catggcaata ttcgctccca ccagtttgcg ggtttcagca tcgtcatcca
    30181 gcccgtcaaa catttgtgcc atccacgccg gaatacgcac gttggtcata tcggcaaatt
    30241 tcttcgcctg tttaaagtta gataccggca aaatccccgg aataatttcc acatcaatgc
    30301 ccgccgatac gcagcggtca cgaaaacgca ggtagctttc gacatcaaag aagaactgag
    30361 taatcgcgcg gttggctccg gcatcgactt tgcgtttcag gttgagcaaa tccgcctgag
    30421 cgctttttgc ttccgggtga acttccggat acgccgccac ggagatatcg aaatctgcca
    30481 cttcttttaa cagcgtcacc aggtcagaag catacatttc cggcttacca cttcccggcg
    30541 gcagatcgcc acgcagcgcc acgatatgac gaataccgtt attccagtag tcgcgtgcaa
    30601 tggtgcgcag ctcgtcgggc gtcgcatcaa tgcaagtaag atgcggtgcc gcttccagac
    30661 cagtgcgatc tttaatgcct ttaataatgc tgtgcgtacg gtcgcgctcg ccggagttcg
    30721 cgccataggt caccgataca aacttcggtt tcaggctgct aaggcgatcg atggagttcc
    30781 acagggtctg ctccatttca ctggtacgcg gcgggaaaaa ctcgaacgaa acgttaatct
    30841 gcccctggac ttctgccagg ctctgattca gggcatcccg ctggctggcg tgaaaaaagc
    30901 tcatacctta cctcatcaat cgcttgtagt tatatgttgt gttatgcaca tctatccatt
    30961 tagacgtcca gatgtaaaga tgaaggaaaa gccgaagggc gtcaaccgaa aaatgaccaa
    31021 tgatggtgag gaaaaatcag ggaagatgaa aaaacttcag gatggtaaga aaaagaaaat
    31081 ccggtatgca aagcaaaccg gactgcaaat ataaactaac catgtactta ttgcttcttg
    31141 ccggattcgt cgtaaacgct tcatccggcc taatactatg ataattcaat atatttcatt
    31201 agaaaccata gacccgaaaa catgagtacc gggcattatt aaatttctga aattacaaca
    31261 actgtgccag ccggttgata tccgactgaa tcgccccggc ggtgacgtcg cgcccagcgc
    31321 caggcccacg gatcaccagc ggattatcgc ggtaccagcg gctttcgatg gcaaagacgt
    31381 tatcgcatgg cagcagtgat gccagcggat ggtcttcacg caccgcttcc acgcccacac
    31441 gcgctttgcc attggcatcg aaacgcgcca cgtagcgcag caccagcccc atttcgcggg
    31501 ccgcttccag ccgttgcacc atctgctcgt taagttcatc gccattttca aagaaatggt
    31561 cgatgctgcc gccttcgcaa tgagcaggca ccagcgattc cacacgtacc tggtccggtt
    31621 caatgttgta acccgcttca cgcgccagaa ttaccagctt gcgcattacg tctttgccgg
    31681 agaggtcgtc acgcggatca ggctcggtta agccctgctg ccacgcctga tccaccagct
    31741 cggtaaacgg cacgctaccg tcgaattgca ggaacagcca ggagagcgtg ccggagaaga
    31801 tcccgctgat cgacaaaata gtatcgccgc tgtcgatcag atcgcgcacg gtgtggttga
    31861 tcggcaagcc cgcaccgacg gtggcattgt acagccagtg acgcccggtt ttttcgaagg
    31921 cgtcgtggat ctggcgatat ttattgctgt cgctggctcc cgccagtttg ttggcgctga
    31981 taacgtggaa accgtggctg gcgaaatcaa gatactgatc agcaagctgc tggctggcgg
    32041 taacgtccag caccactaaa tcatcatacg gatgggcgcg catccacagg aacaacgact
    32101 cttcatcctg ctcaaccgct tcatcgttga agaaggctaa cgcgcggctg gcgtccagcc
    32161 cgtcatagct caacaggctg cggcggctgt ccaccacacc tgccagcaca aactcaaagc
    32221 cggtacgtgc cgaaagcgtg ctctgctcac gggcgaacag ttccagccaa cgggaaccga
    32281 tattgccctt accgaacaat accaggccga tgcgtttttc tgcgcggaag acggactgat
    32341 gcagcccctg aatcaggctt tcggtcgggc cggtgcgcag tactgccacc aggctgatgc
    32401 cgtcatcgga ctgccaggta aattcgaccg gctggccttt cagttgctgc cagaagcggt
    32461 ggcaatgcag cgggttacgg gtgacgcctg caccgaccat cgccaccagc gccagcccct
    32521 gacgcaggcg cagttcgcca ggtaatcccg cttcgtcgag gattttcagc gcactgtcgg
    32581 ccacttctga ggtgtagcaa aattgcagca actggcgatc gttatgtacg ccaaccgcca
    32641 gcgggcgtac ctgcgcgcgt ttcaggattt ggtcgatctc tttatgcgcc agtttgaaat
    32701 cctgactggc gggcacctga aactcaatca aacagacatc atcgtggctg gtgacaatac
    32761 gcgcaccagt accggaggcc agcacgcgtt caatgcgcgt ggaaccttga tccggcgtgt
    32821 agctacagcg cagttgcagg tcgatttcgc tgccagaaac cggctgtaaa gtacgggcgt
    32881 gaagaacggg agccgccagg cgcgccagtt cgctggcctc atccagacgc agcaacggca
    32941 gcaggcaggc atctttcact ttacgcgggt cggcactgta taccccggcg acgtcgctcc
    33001 agatggttac gcgagaaaca cccgccagcg caccgatttg tgtcgcggaa tagtcggaac
    33061 cgttacgccc cagcagcacc gtttcaccgg cgttgttgcg gctgataaat ccggtcacca
    33121 ccagacgttt gcccggatgt tgcaccagca gctgttgcag caacgggtaa gaaagccctt
    33181 catcaacctg cggttgtgcg gcgcgttcag cgcgtaaaaa ctcgcgggca tcaagccagg
    33241 ccgctggcag cccttgttga ttaagtaccg cagacatcag gcgtgccgac cacacttcac
    33301 cgtgccccac cacttccgca tacactgcgt cgttaatacc gctgtcgagc agcgccgcca
    33361 ggcgctcaag gtcgctgaca aaagcgctaa tgaggctatc ggcttcttca gcgggtagca
    33421 gaccgctaat cagatcgcac tgatagcgac gcagcgtttg ttgaacctga tgcgcagaga
    33481 gacgatcggt ctggcttagt ttcaaccagt taatcaactg gttagtggtg ctaccggcgg
    33541 cggaaaccac catcatatcg tcaggctgag agtactccgc cataatgccc gcgacacgca
    33601 aataacactt cacatcagcc agactactgc caccaaattt atgcagctga cgacctttcg
    33661 cccctgcctg cgcaatcaca ctcattttta ccccttgttt gcagcccgga agccattttc
    33721 caggtcggca attaaatctt cgccatcttc aataccggtg gagatacgca gcagcgtctc
    33781 ggagatcccg gcggcagcac gcgcttctgg tgccatgcct gcatgtgtca tggttgcggc
    33841 gtgagagatt aaactttcca ctccccctaa tgattccgcc agcgtaaaca acgacagccc
    33901 gctcaggaaa cgacgcagcg tctgctcatc gccatccagt tcaaaactca acattgcgcc
    33961 aaagcctttt tgctgacgcg cggcaatttc atgcccctga ttttccggca gcgacgggtg
    34021 atacagtttt ttcaccaacg gctgggtttg cagatatttc acaatcgcct gcgcgttacg
    34081 ctgggccaac tccatgcgcg gcaccagcgt tcgcaacccg cgtagtagca gatagctgtc
    34141 aaacgcgccg cccgtcacgc caatattgtt tgcccaccag gcaagttcag tgacaacgtc
    34201 cgggtcttta gcaatcacca cgccggccac cacgtctgag tgaccgttca gatatttcgt
    34261 gcatgaatgc aacaccagat cggcacctaa tgccagcgga ttttgtaatg ccgggcttaa
    34321 gaaggtgtta tccaccacgc tcaccgcccc gacttccctt gccagatggc agattttcgc
    34381 aatatccacg acgcgtaaca atggattact tgggctttct accagtacca gtttgggttt
    34441 ttctgccagc gctgcccgta atgcctgttc atcgccttga tcaacaaaca acacgcgata
    34501 gcaaccacgt ttcgccaggc tgtcgaacag gcgatagcta ccgccgtagc agtcgtgcgg
    34561 cgctaccagc agatcgccag gtttcaaaaa gacggtcgtt accaggtgaa tcgcggacat
    34621 gccggtatta gtaagtactg caccagcacc gccttccagt tctgccagtg ctcgctgaac
    34681 cacatcgcgc gttgggttgc cacgacgcga gtaatcatgc gcgcgcggtt cattaaatcc
    34741 ggtaaagtta taggtactgg aaagatggat cggtgggaca acgcaaccat actgttcgtc
    34801 gtcatttaac ccgctacgca ctgcgatggt ggcctgttta cgcgtcatgt gatgaagttc
    34861 cctgggcttg gtcggtgaaa tgtcaggcac cagagtaaac attgtgttaa tggacgtcaa
    34921 tacatctaga catctaaact tctttgcgta tagattgagc aaatcccaaa tagccgttaa
    34981 aattatatgc attatcacgc cgacaggtgc attacacgat gtcacggtaa cgcctgtacg
    35041 gtaaactatg cgggtttacg gtcagtaccc acataaattg tgtggtctgg tctcaattta
    35101 ttgacgaaga ggattaagta tctcatggct gaatggagcg gcgaatatat cagcccatac
    35161 gctgagcacg gcaagaagag tgaacaagtc aaaaagatta cggtttccat tcctcttaag
    35221 gtgttaaaaa tcctcaccga tgaacgcacg cgtcgtcagg tgaacaacct gcgtcacgct
    35281 accaacagcg agctgctgtg cgaagcgttt ctgcatgcct ttaccgggca acctctgccg
    35341 gatgatgccg atctgcgtaa agagcgcagc gacgaaatcc cggaagcggc aaaagagatc
    35401 atgcgtgaga tggggattaa cccggagacg tgggaatact aaagcaaaaa agagcggcgc
    35461 ggagtggaat cgcctgatgc gctacgctta tcaggcctac gtcatattgc aatttattga
    35521 atttgcacga acttgtaggc cggataaggc gttcacgccg catccggcat aaacaacgag
    35581 cacgttgtct gcgacccacc gctttttata catggacgtt taactatgaa aaacaggctg
    35641 ctgatcctca gcctgctggt ttctgtacct gcctttgcct ggcagccgca aaccggcgac
    35701 atcatctttc agatctctcg ctcatcgcaa agtaaagcga tccaactggc gacccattcc
    35761 gattacagcc acaccggtat gctggtgata cgcaacaaaa agccctacgt ttttgaagcg
    35821 gtcggcccgg taaaatacac cccgctcaag cagtggatcg cccatggtga aaagggtaag
    35881 tacgttgttc gccgcgttga aggcggactg agtgttgaac aacagcaaaa actggcgcaa
    35941 acggcaaaac gttatctcgg taaaccgtat gatttcagct tctcatggag cgacgatcgc
    36001 cagtactgtt cggaagtggt gtggaaggtt taccagaacg cgctgggaat gcgcgtgggc
    36061 gagcagcaga agttgaaaga gttcgatctc agcaacccgc tggtccaggc gaagctcaaa
    36121 gaacgctacg gcaaaaatat tccgcttgag gaaacggtag tttcaccaca agccgttttc
    36181 gacgcgccac aactcaccac ggttgccaaa gagtggccgc tgttttcgtg gtaatggatg
    36241 atgaaaaaaa taattcccgc cgtagtctgt ttgttgcttt gtagttgcac gacctttccg
    36301 caagctgtcg ctccggtaaa cggtgatgcc tccaattagt tgaattaagg atataatact
    36361 cgcttttaag gttgtttcat ggtcacagtt aaccttcatt gcccccgttg ccagtccgtt
    36421 caggtttacc gtcacgggca gaacccaaaa agacatgatc gattccgcgg ccgtgactgt
    36481 caccgggtat ttcagctcag ttactgttac gagccccgta aaccgggagt taaagaccaa
    36541 atcgctgaaa tgactttcaa cggtgccggg gttcgcgata ctgcccgtac gctgaaaatc
    36601 ggcattaata cagtcatccg aactttaaaa aactctcgcc acgaagaata acgtcatccc
    36661 cggtcgctca tgctgatgtg gcactcatct gcaaacttag tcagcaatgg agctttgtcg
    36721 gcagtaaagc gcgacagcac tggctctggt acgtgtacaa cacgaaaact ggtggtgttc
    36781 tggcctacac ttttggcccc cgggctgacg aaacctgtcg tgaattgcgg gcgctgctta
    36841 cccttttacc atcggcatgt tgaccactga cgaatggggt agttacgcta gagagttacc
    36901 gaaggagaag catctgactg gtaagatttt tactcagcac attgagcgta acaatctgac
    36961 cctgagaacc cgaatcaaac gtctggcccg cagaaccatc tgtttctcgc gttctgtaga
    37021 acttcatgaa aaagtcatcg gtgcttttat cgaaaatata tgttctacta attggagtca
    37081 tcacccttct gataagcttt ccttggatga ttaccagact gaatacacag ccaaagattt
    37141 ttacctcatt ctgctctttt ttctcaaatg acatatatta tattttacat catattcata
    37201 ggcatatgac attcccccgc cccccattat gtcattgtgg gttgatgttg ggctttcata
    37261 aaaaaaaaca tctttttttg aactagcatt aattatattt actttatata ttaaaatatt
    37321 ttctccatct ggaaatattt tagccacaca atataaagca gctgaatccg aattacttaa
    37381 aaatatatta tcatcaccca taactccagc cattaaatga ctggagacta aagcaaggaa
    37441 ataacaacat tgtattgcct tcattttaat tactcccttg cctgataatt gtaactccat
    37501 tccctttagc tttgggatta tattttagat aggtgttatt tggtgttccc aagtaactac
    37561 tgtattcttt ttttcccatt ccattcatat aagaattcgt taaatctttg cttgaaaaat
    37621 ttagactatc ataaacatca ttttcttttg ttactttatt tcctttatcg tcagagtaaa
    37681 aaccgtgagt gtgataatcg cctactctct ctgaaccttc agggcatttt atatttcgca
    37741 agtctacact atcattatca tttgaactta tcggattcat ggggaaatat tcaccttgct
    37801 tcttacatat tagtccacca tattctttat tctcgttaat tgattctcca ttacacattg
    37861 ccaatgcaaa tattccaata tcatctatat tttttgattt aaattcatat aaacctaatg
    37921 gatccatgct attcaccggg ctcagcggat atgtatacag gttccatccc cccttcagcc
    37981 caatcgga
//
LOCUS       DAMZET010000025        37866 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.025, whole genome shotgun sequence.
ACCESSION   DAMZET010000025 DAMZET010000000
VERSION     DAMZET010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.025"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..424)
                     /gene="mdtL"
                     /locus_tag="POB34_001382"
     CDS             complement(<1..424)
                     /gene="mdtL"
                     /locus_tag="POB34_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HDI5918365.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPV"
     gene            complement(557..1804)
                     /gene="tnaB"
                     /locus_tag="POB34_001383"
     CDS             complement(557..1804)
                     /gene="tnaB"
                     /locus_tag="POB34_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HDI5918366.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYPILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            complement(1895..3310)
                     /gene="tnaA"
                     /locus_tag="POB34_001384"
     CDS             complement(1895..3310)
                     /gene="tnaA"
                     /locus_tag="POB34_001384"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HDI5918367.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            complement(3531..3605)
                     /gene="tnaC"
                     /locus_tag="POB34_001385"
     CDS             complement(3531..3605)
                     /gene="tnaC"
                     /locus_tag="POB34_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HDI5918368.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            complement(3848..5212)
                     /gene="mnmE"
                     /locus_tag="POB34_001386"
     CDS             complement(3848..5212)
                     /gene="mnmE"
                     /locus_tag="POB34_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDI5918369.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(5318..6964)
                     /gene="yidC"
                     /locus_tag="POB34_001387"
     CDS             complement(5318..6964)
                     /gene="yidC"
                     /locus_tag="POB34_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDI5918370.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(6967..7224)
                     /gene="yidD"
                     /locus_tag="POB34_001388"
     CDS             complement(6967..7224)
                     /gene="yidD"
                     /locus_tag="POB34_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDI5918371.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(7188..7547)
                     /gene="rnpA"
                     /locus_tag="POB34_001389"
     CDS             complement(7188..7547)
                     /gene="rnpA"
                     /locus_tag="POB34_001389"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDI5918372.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(7564..7704)
                     /gene="rpmH"
                     /locus_tag="POB34_001390"
     CDS             complement(7564..7704)
                     /gene="rpmH"
                     /locus_tag="POB34_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDI5918373.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(7934..8014)
                     /locus_tag="POB34_001391"
     CDS             complement(7934..8014)
                     /locus_tag="POB34_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918374.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            8311..9714
                     /gene="dnaA"
                     /locus_tag="POB34_001392"
     CDS             8311..9714
                     /gene="dnaA"
                     /locus_tag="POB34_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDI5918375.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            9719..10819
                     /gene="dnaN"
                     /locus_tag="POB34_001393"
     CDS             9719..10819
                     /gene="dnaN"
                     /locus_tag="POB34_001393"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDI5918376.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            10819..11892
                     /gene="recF"
                     /locus_tag="POB34_001394"
     CDS             10819..11892
                     /gene="recF"
                     /locus_tag="POB34_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDI5918377.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            11921..14335
                     /gene="gyrB"
                     /locus_tag="POB34_001395"
     CDS             11921..14335
                     /gene="gyrB"
                     /locus_tag="POB34_001395"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDI5918378.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            14575..14973
                     /locus_tag="POB34_001396"
     CDS             14575..14973
                     /locus_tag="POB34_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HDI5918379.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            15088..15900
                     /gene="yidA"
                     /locus_tag="POB34_001397"
     CDS             15088..15900
                     /gene="yidA"
                     /locus_tag="POB34_001397"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDI5918380.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(15946..16602)
                     /locus_tag="POB34_001398"
     CDS             complement(15946..16602)
                     /locus_tag="POB34_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918381.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDRVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            16880..17569
                     /gene="dgoR"
                     /locus_tag="POB34_001399"
     CDS             16880..17569
                     /gene="dgoR"
                     /locus_tag="POB34_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HDI5918382.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            17566..18444
                     /gene="dgoK"
                     /locus_tag="POB34_001400"
     CDS             17566..18444
                     /gene="dgoK"
                     /locus_tag="POB34_001400"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HDI5918383.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            18428..19045
                     /gene="dgoA"
                     /locus_tag="POB34_001401"
     CDS             18428..19045
                     /gene="dgoA"
                     /locus_tag="POB34_001401"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HDI5918384.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            19042..20190
                     /gene="dgoD"
                     /locus_tag="POB34_001402"
     CDS             19042..20190
                     /gene="dgoD"
                     /locus_tag="POB34_001402"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HDI5918385.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            20265..21602
                     /locus_tag="POB34_001403"
     CDS             20265..21602
                     /locus_tag="POB34_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5918386.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(21599..22682)
                     /gene="cbrA"
                     /locus_tag="POB34_001404"
                     /pseudo
     CDS             complement(21599..22682)
                     /gene="cbrA"
                     /locus_tag="POB34_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
     gene            22783..23997
                     /locus_tag="POB34_001405"
     CDS             22783..23997
                     /locus_tag="POB34_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HDI5918387.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHLREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(23999..24331)
                     /locus_tag="POB34_001406"
     CDS             complement(23999..24331)
                     /locus_tag="POB34_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDI5918388.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    24571..24643
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            24637..25050
                     /gene="ibpA"
                     /locus_tag="POB34_001407"
     CDS             24637..25050
                     /gene="ibpA"
                     /locus_tag="POB34_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HDI5918389.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            25162..25590
                     /gene="ibpB"
                     /locus_tag="POB34_001408"
     CDS             25162..25590
                     /gene="ibpB"
                     /locus_tag="POB34_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HDI5918390.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            25787..27448
                     /locus_tag="POB34_001409"
     CDS             25787..27448
                     /locus_tag="POB34_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HDI5918391.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGLPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(27445..28161)
                     /locus_tag="POB34_001410"
     CDS             complement(27445..28161)
                     /locus_tag="POB34_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI5918392.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            28457..30073
                     /locus_tag="POB34_001411"
     CDS             28457..30073
                     /locus_tag="POB34_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDI5918393.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGQFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            30073..30711
                     /locus_tag="POB34_001412"
     CDS             30073..30711
                     /locus_tag="POB34_001412"
                     /inference="COORDINATES: protein motif:HMM:NF014148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918394.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQERFPLRALKFYDNDGAR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHWWSAISRSFRKG"
     gene            30711..30887
                     /locus_tag="POB34_001413"
                     /pseudo
     CDS             30711..30887
                     /locus_tag="POB34_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082801.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            complement(30884..31777)
                     /locus_tag="POB34_001414"
     CDS             complement(30884..31777)
                     /locus_tag="POB34_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDI5918395.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            31944..33659
                     /locus_tag="POB34_001415"
     CDS             31944..33659
                     /locus_tag="POB34_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HDI5918396.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            33656..35149
                     /locus_tag="POB34_001416"
     CDS             33656..35149
                     /locus_tag="POB34_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDI5918397.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(35196..35645)
                     /locus_tag="POB34_001417"
     CDS             complement(35196..35645)
                     /locus_tag="POB34_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918398.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            35755..36102
                     /locus_tag="POB34_001418"
     CDS             35755..36102
                     /locus_tag="POB34_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HDI5918399.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            36092..36454
                     /locus_tag="POB34_001419"
     CDS             36092..36454
                     /locus_tag="POB34_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HDI5918400.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            36451..36948
                     /locus_tag="POB34_001420"
     CDS             36451..36948
                     /locus_tag="POB34_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDI5918401.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(36956..>37866)
                     /gene="emrD"
                     /locus_tag="POB34_001421"
     CDS             complement(36956..>37866)
                     /gene="emrD"
                     /locus_tag="POB34_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HDI5918402.1"
                     /translation="TSSLTVLIAASAMQGMGTGVGGVMARTLPRDLYERTQLRHANSL
                     LNMGILVSPLLAPLIGGLLDTMWNWRACYLFLLVLCAGVTFSMARWMPETRPVDAPRT
                     RLLTSYKTLFGNSGFNCYLLMLIGGLAGIAAFEACSGVLMGAVLGLSSMTVSILFILP
                     IPAAFFGAWFAGRPNKRFSTLMWQSVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFF
                     FGAGMLFPLATSGAMEPFPFLAGTAGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLL
                     MTLMGLLIVLCWLPLATRMSHQGQPV"
BASE COUNT         9094 a         9623 c        10017 g         9132 t
ORIGIN      
        1 acaccggaat gatgcaggta ataccgttga gtaatgacag cacttttgcc cgacgtcgat
       61 catccagcgt gtcgcgcaaa atggcgaacg ccaccacgta acaacagccc gcgcccaacc
      121 cctgtagaaa gcgccctgca agaaataacg tgctggtttc agccagtgaa cagaacaccg
      181 aggcgataat aaatagcgcc gcgccgggga tggcgaccgg ctttctccct gaacgatcgg
      241 ccactttacc ggcaaataac atcgcagctg ccatcccagc cagataaaca gagaacgcaa
      301 tatgcaactg cgcttcgctg gcattgagat cggcggcgat gcgcggtaaa ccaacgaggt
      361 acatatcaat cccggcggga taaagtaaaa ccagggcaaa actacaaatc aaaaagcggg
      421 acatgggcta aagcgtcctg atagtaaggt gacgcaaagc atacgtggtg aaggagaatc
      481 aggcgagttg ccatttggac ataagtgatt tcctgacagg caagaagcca gcgaaatggc
      541 tggcttcctg aaggatttag ccaaatttag gtaacacgtt aaagacgtta ccgaaccagc
      601 ataaaattac ggttatacca aagagaatga caatcgccgg aataagatta ccgccccaga
      661 ccgtgaacat ctgattggga aacttcttgc gagctttgat tgccagcact gcgggaataa
      721 tgaccgccca gatggtggcg cacagtccgg caccgccgat cccgtaaata aagccgttcg
      781 ggaagatcag atacaacaac gcgggtggca ggaaggttaa cagcacggtt ttgaaacgcc
      841 cgccgtggga gttatcaatc ttaaacagat ccgccagata atcgaacaat cccagcgtga
      901 caccaaagaa cgaactggca acagctaagt tggagaacac cagcaggcaa aactcgataa
      961 tcccatgctg tttggtgccg aggaacgatt tcaccagcga atcaacgttg ccgcctgagg
     1021 agattatcgc cttaaagctt tcgcgcggaa tattccccat cgtgcaatag agccagaaga
     1081 gataaatcac cagcgccagc agcgaaccaa agaccacgct tttgattaac ttatctttgc
     1141 gtttgccata gcaaataatc aggctgggaa tattgccgtg gaaaccaaat gacgccagac
     1201 acaccggcaa agccataaag atatacggga agtaagacgt tcccgcagtg gtgctggtgg
     1261 catcgcgcag aatggggtaa tcgacctgga agaagaaaga accaaacacg atcacaaagg
     1321 agataatctt cagcccgagg aataatgagg taatacgact ggcggctaac gaacttatcc
     1381 acaatacgct ggcaacgaaa atggctgtgc agatcccgac aatacgcgga ttagcgtgat
     1441 aacccaaatt cattgatatc gtttcactaa tgatcgcacc attagcagag atataggcat
     1501 aagtgaggat atagagaacg aaggcaacgg taataccgct gataatgttc caggtgttac
     1561 cgattaaatc tttggtgatg gtgttaaaac tggagccgac gggataattt aaatttgctt
     1621 ctaataacaa taacccggaa tgaagcattg aaaaccaggc aatgataagg ataaaggcac
     1681 cccagaaaaa ccaggcaccg gcaagatcaa caggtaaagc aaacatacct ccaccaatta
     1741 ctgtacctgc tataaccata acaccccaaa atgcagagtg ctttttttca gcttgatcag
     1801 tcatgatgcc acctttagag gaaggctatt tttgttattg aggatgtagg gtaagagagt
     1861 ggctaacatc cttatagcca ctctgtagta ttaattaaac ttctttcagt tttgcggtga
     1921 agtgacgcaa tactttcggt tcgtaggtaa aggttaatcc tttaatattc gccgcgttct
     1981 ctttcacatg tttaaaggct tcaataatga agtccatatg tgtttgagta tatgttgcgc
     2041 gcggaatggt taaacgcagc agttcagccg ggcatggcag ttgtttaccg gttttcggat
     2101 cgcggcctaa caggaaagaa ccaatttcta ccgcacggat accggcgact ttatacagct
     2161 cgcaggccag cgcctgtgcc gggaactgat ctgccgggat atgcggcaac agtttacctg
     2221 catcaacgaa tgccgcgtga ccgcccgcct gctggcagac aacgccaatc tcttccaggc
     2281 catcgaccag atactgcacc tgcgcaatac gataagccag ccagtccaga ttcatgccgt
     2341 catacagacc taccgccaga cgctccatcg cgccgccttc cagaccacca tatgtcggga
     2401 agccttcctg caccacgcaa agggttctgc attcggtgta cacatcaaag aagctgtcgt
     2461 ctttcatgca cagcaagccg cccatcggca ccatcgcatc tttcttggcg gacatcgcca
     2521 gcatatcggc atatttgtag gtttcgcggg tgatctgctc gatggtccag tccttgtatt
     2581 ctgcttcacg ctgcttgatg aaataggcgt tttcagcaaa gcgtgcggag tccattacca
     2641 ccggaatatc gtatttcttc gcgatgctgt acatcgcttt taagtttgcc agtgaaaccg
     2701 gctgaccacc tgcagagtta ctggtgatgg ttgcaacgat atacggcacg ttattcgggc
     2761 caacttcttc aataccgcgt tctaatcctt cgaggtcaaa gttgccttta aagtcgtaac
     2821 gcacgcccgt atcgaaggct tctttgatat agacgttacg cacggtacag ccgttaatct
     2881 ggctatggcc ctgcgtggta tcaaagaaat agttagagaa tgccaccatt ttgctgcgat
     2941 ccaggccttt ttcctgctcg cgttttttaa tcagtaccgg aatatagatt tgctctgcgc
     3001 cacggccctg gtgagtcgga atagtgtatt gataaccaaa gatatttttc actgactcgg
     3061 ctaacgcata gtagctgcgg ctgccgctgt aggcttcgtc gccgcgcatc atcgctgcct
     3121 gcatactttg ggttaccgcc ccggtgccgc tgtcggtcag taaatcgata aacacatctt
     3181 cgctatccag caggaacggg ttcataccgg atttaataat tgcttcttca cgataagcgc
     3241 gagtagtacg ttttactggc tcaataacac gaatgcggaa cggttcaggg agatgtttaa
     3301 agttttccat tacataatcc ttcatttatt ttaattacag tgatccctgt gaatattaca
     3361 tctgcaatat ccattaattc gccatgtata tatacaagca ccatcattta ctaaaataag
     3421 taaataaacg agagatggcc tttgcaaaag gcaaaattaa gatatagcaa actaatcgtt
     3481 cacattgaat ctaatcggtt tggctctggt gatggctaca gaagggtaaa ttaagggcgg
     3541 tgatcgacaa tcttattgtc gatattgaac cattttgagg tcacacatat atgtaagata
     3601 ttcataatgc acttatcctc gaaagacacg aggctacagt ataatactca agaattttgt
     3661 ctattctaaa attgtttaaa tgtgaatcga atcacaatcg ttcggggagc aatattaaat
     3721 gctttttttt aagattataa attacgtaag aattttaatg tttaaaaaaa gggttgagga
     3781 ataacaggag tgctaacaat gaagcgaagc cgcatctgac agtcagaata cggcttcgta
     3841 agcgcggtta cttaccaata cagaagctgg agaaaatccg ccccagcagg tcatcagaag
     3901 taaattcccc ggtgatttcg cttaagttct gctgtgccag gcgcaactct tccgccagca
     3961 gttcgcccgc ccaggcacct aacagttgcg ctttaccttg ctgcaaatgc tctgccgcct
     4021 gttccagcgc ctgtaggtgg cgacgacgcg ccaggaagcc gccttccatg ttggtgtcaa
     4081 agcccatgct ctgtttgaga tggttacgca gcacgtccac gccttcacca gtccttgccg
     4141 agagacgaat taacgcgtga ccgttcactt cactcattcc cagcgtttcg ccggtgatat
     4201 cggctttatt gcgcaccacg gtgatcggca gtttcgctgg cagacgggca ataaattccg
     4261 gccagatctc tgccggatcc acggcgtctg ttgtggtgcc atcgaccata aacagcacgc
     4321 ggtcggcctg ttcaatttcc tgccacgcgc gctcgatacc aatacgttct acttcgtcac
     4381 tggcttcacg tagcccggcg gtatcgatga tatgcagcgg cattccgtca atgtggatat
     4441 gctcacgcag cacgtcacgc gtagttccgg cgatatcggt tacgattgcc gcttcacgcc
     4501 ccgccagcgc gtttaacagg ctcgatttac cggcgttagg acgtccggca atcaccactt
     4561 tcatcccttc gcgcaacaaa ctaccctgac gtgcttcagc acgcactgca tcaagatcgg
     4621 caataacgtc attgagctgg gcttcaattt ttccgtcgga gaggaaatcg atctcttcat
     4681 cggggaaatc aattgccgct tcgacgtaaa tgcgcaagtg ggtgagggct tctaccagat
     4741 gattaacccg tgcggagaat gcgccttgca gcgagttaag tgccgaacgg gccgcctgtt
     4801 ccgaactggc gtcgataaga tcggcaatcg cctcggcctg ggctaagtca agtttatcgt
     4861 taagaaacgc gcgttcggaa aactcaccag ggcgagcaat ccgcaggccg ggaatggtca
     4921 gaatgcgttt taacagcagg tcgaggatca ccggaccgcc atgaccttgc agttccagca
     4981 catcttcgcc ggtgaacgag ttcgggccag ggaaccatag cgcaatcccc tgatcgagca
     5041 cgctgccgtc ggcgtcttta aacggaagat aatcggcgta gcgcggctta ggcagtttac
     5101 ccagcacggt ttcggcaact tcacgggctt tgaggccgga gatgcgcagg atgccaacac
     5161 cgccacgtcc cggaggcgtg gcctgggcta cgatagtgtc attatcgctc atgatgttcc
     5221 tgttgctgtg tgtggcggat gcgcggtgct tatccgccct acgaaaagaa aaaaggcggt
     5281 caactgaccg cccttatttt agcgaaaact caccgaatca ggattttttc ttctcgcggc
     5341 tatgcaggcc acgtttttcc agaccacggt aaatcagctg ctgctgaata atggttacca
     5401 ggttgctgac gatatagtac agcaccagac ctgacgggaa ccacaggaag aacacggtga
     5461 agatgaccgg cataaaggtc atgatcttct gctgcatcgg gtcggtcact gtggtcggcg
     5521 acatcttctg aatgaagaac atcgttacgc ccatcaggat cggcaggatg tagtacgggt
     5581 cctgtgccga caggtcgtgg atccacagtg caaacggtgc ctgacgcagt tcaacggaac
     5641 ccatcagcat gtagtacaac gccaggaaga ttggcatctg gatcagcagc gggaagcagc
     5701 cgcccagcgg gttaaccttc tcagctttgt acagcgccat catttcctgg ctgatacgct
     5761 gtttgtcatc gcccagacgc tcacgcattg cctgaatctt cggctgcagc atacgcatct
     5821 tcgccatgga ggtgtactgc gctttggtca gcgggtacat gatgccacga acgataaagg
     5881 tgatgatgat aatggagaag ccccagttac ccacaaagct atggatccat ttcagcagtt
     5941 tgaacagcgg ctgagagatg aaccacaacc aaccgtaatc aacggtcaga tccaggtgcg
     6001 gagcaacagc tgccattttg tcctggattt ccgggccaac ccacagggtg ctgttcatcg
     6061 cgccagtctg accaggctga accagtaccg gctgagattt atagccgata gcggcgatgc
     6121 cgttacccag attagcggta tagaagttgt tggtaccgtc gttatgcggg atccacgccg
     6181 tcgcgaaata ctgttgcagc atcgccaccc aaccaccttt cgaagagatg ttcaggtttt
     6241 cgttatcggc aatggtatcg aacttgtatt tctcatactt ctcgtcaggc gtggagtacg
     6301 ccgcgccacg gaaggtgtgc agtgcgaagt tgctgcttcc ggtatcgaga tgcggtggca
     6361 gagtgatgga ttgcttcaac tgaccaaagg tggagatttc cagtggtttc tcgccagcgt
     6421 tctgcacgtt gtagttgacg ttgacagcgt aatcaccacg tttcaggaca aacgttttgg
     6481 taaacgtgtt gcctgccgcg tcggtatacg tcatcggcac ctgcagttcg ttttgacctt
     6541 cagccagcac ataagcgtct ttttcaacgt tatacagcgg acgcgggccg ttagccgggt
     6601 tatccgggcc atcacgaccg gtcagaccgc tctgtgcctg ataaataaac tgcggtgaag
     6661 tttccagcag ctggaacggc tgggtagagt tcagctcttt cgggtaagcc ggcagcagag
     6721 cttgctcaac atcaccacca cgggtgttga tggtcagatc aagcacgtcg gtcttaaccg
     6781 agatcagttt cccctggcca ctggccggta cgccctggtc ggcggcgcta cccgctgcgg
     6841 tggtcgttgt ctgcgtggtc tgttgggcct gaggttgcgg gtttttatcc tgctcccagg
     6901 cttgccagat catgaaagac acgaacagca aagcgatgac taaaagattg cgttgcgaat
     6961 ccatcgttag tgttctctgg tatcaaatgg tccgggcggg acgggatcgt caccaccagg
     7021 gtgtaaaggg tggcatttta atacgcgttt caccgtcaac caactgcctt ttatcactcc
     7081 aaacctgcgc aatgcctcaa ttccgtagct tgaacaggtt ggagtgaaac gacaatgcgg
     7141 cccgagtagc ggactaatca ggcgttgata gacccgaatg agggctatca ggacccgcga
     7201 gccaggcgac agtggcggcg ccataatttt tccaacgctt ccgagagagc acggttatcg
     7261 aggtcggcaa cccctttttt cgccaccacc acgaaatcca tagccgggag ttcatgttgg
     7321 cgcagacgga agctttcacg cgtcagacgt ttaatccgat tgcgttcatg ggcgcgtcga
     7381 acgtttttct tggcgactgt aagaccgata cggggatgcc ccagcgaatt caggcggccg
     7441 agaatggtaa tttgcggcgt gccagcccgt tgtggctgct ggaagacgaa tgtgaattga
     7501 ctgggagtta acaagcgtaa ctccctggga aatgcgagct taaccactca ggggttagct
     7561 ttattactta gaaacggtca gacgagcgcg gcctttagca cgacgacgtg ccagaacctg
     7621 acgaccattt ttagtagcca tacgagcacg gaagccgtga gaacggttgc gcttcagtac
     7681 agacggttga aaagtgcgtt tcatggcgat ttctacctaa acttgaataa attcaatggc
     7741 tttattggat atccgccgaa aaatgaaacg atggacaccg aagccatggg tgattaaaga
     7801 ggccggattg taataattgt acactccgga gtcaattctc tttccttatt taccgcgctt
     7861 ttccgcacct tttcgcaggg aaaatgtacg acctcacacc agtgaaaacc agcatggcgc
     7921 gccgggtgga ggattatacg ggctgatggg taaagcgcaa ggatcgtcct ggatctttat
     7981 tagatcgatt aagccaattt ttgtctatgg tcattaaatt ttccaatatg cggcgtaaat
     8041 cgtgcccgcc tcgcggcagg atcgtttaca cttagcgagg tctggaaagt cctgtggata
     8101 aatcgggaaa atctgtgaga aacagaagat ctcttgcgca gtttaggcta tgatccgcgg
     8161 tcccgatcgt tttgcaggat cttgatcggg catataaccg cagacagcgg ttcgtgcgtc
     8221 accctcaagc agggtctttt cgacgtacgt caacaatcat gaatgtttca gccttagtca
     8281 ttatcgactt ttgttcgagt ggagtccgcc gtgtcacttt cgctttggca gcagtgtctt
     8341 gcccgattgc aggatgagtt accagccaca gaattcagta tgtggatacg cccattgcag
     8401 gcggaactga gcgataacac gctggccctg tacgcgccaa accgttttgt cctcgattgg
     8461 gtacgggaca agtaccttaa taatatcaat ggactgctaa ccagtttctg cggagcggat
     8521 gccccacagc tgcgttttga agtcggcacc aaaccggtga cgcaaacgcc acaagcggca
     8581 gtgacgagca acgtcgcggc ccctgcacag gtggcgcaaa cgcagccgca acgtgctgcg
     8641 ccttctacgc gctcaggttg ggataacgtc ccggccccgg cagaaccgac ctatcgttct
     8701 aacgtaaacg tcaaacacac gtttgataac ttcgttgaag gtaaatctaa ccaactggcg
     8761 cgcgcggcgg ctcgccaggt ggcggataac cctggcggtg cctataaccc gttgttcctt
     8821 tatggcggca cgggtctggg taaaactcac ctgctgcatg cggtgggtaa cggcattatg
     8881 gcgcgcaagc cgaatgccaa agtggtttat atgcactccg agcgctttgt tcaggacatg
     8941 gttaaagccc tgcaaaacaa cgcgatcgaa gagtttaaac gctactaccg ttccgtagat
     9001 gcactgctga tcgacgatat tcagtttttt gctaataaag aacgatctca ggaagagttt
     9061 ttccacacct tcaacgccct gctggaaggt aatcaacaga tcattctcac ctcggatcgc
     9121 tatccgaaag agatcaacgg cgttgaggat cgtttgaaat cccgcttcgg ttggggactg
     9181 actgtggcga tcgaaccgcc agagctggaa acccgtgtgg cgatcctgat gaaaaaggcc
     9241 gacgaaaacg acattcgttt gccgggtgaa gtggcgttct ttatcgccaa gcgtctacga
     9301 tctaacgtac gtgagctgga aggggcgctg aaccgcgtca ttgccaacgc caactttacc
     9361 ggaagggcga tcaccatcga cttcgtgcgt gaggcgctgc gcgacttgct ggcattgcag
     9421 gaaaaactgg tcaccatcga caatattcag aagacggtgg cggagtacta caagatcaaa
     9481 gttgcggatc tcctttccaa gcgtcgatcc cgctcggtgg cgcgtccgcg ccagatggcg
     9541 atggcgctgg cgaaagagct gactaaccac agtctgccgg agattggcga tgcgtttggt
     9601 ggtcgtgacc acacgacggt gcttcatgcc tgccgtaaga tcgagcagtt gcgtgaagag
     9661 agccacgata tcaaagaaga tttttcaaat ttaatcagaa cattgtcatc gtaaacctat
     9721 gaaatttacc gtagaacgtg agcatttatt aaaaccgcta caacaggtga gcggtccgtt
     9781 aggtggtcgt cctacgctac cgattctcgg taatctgctg ttacaggttg ctgacggtac
     9841 gttgtcgctg accggtactg atctcgagat ggaaatggtg gcacgtgttg cgctggttca
     9901 gccacacgag ccaggagcga cgaccgttcc ggcgcgcaaa ttctttgata tctgccgtgg
     9961 tctgcctgaa ggcgcggaaa ttgccgtgca gctggaaggt gaacggatgc tggtacgctc
    10021 cgggcgtagc cgtttttcgc tgtctaccct gccagcggcg gatttcccga acctcgatga
    10081 ctggcagagt gaagtcgaat ttaccctgcc gcaggcaacg atgaagcgtc tgattgaagc
    10141 gacccagttt tctatggcgc atcaggacgt tcgctattac ttaaatggta tgctgtttga
    10201 aaccgaaggt gaagaactgc gcaccgtggc aaccgacggc caccgtctgg cggtctgttc
    10261 aatgccaatt ggtcaatctt tgccaagcca ttcggtgatc gtaccgcgta aaggcgtgat
    10321 tgaactgatg cgtatgctcg acggcggcga caatccgctg cgcgtacaga ttggcagcaa
    10381 caacattcgc gcccacgttg gcgactttat cttcacctcc aaactggtgg atggtcgctt
    10441 cccggattat cgccgcgttc tgccgaagaa cccggacaaa catctggaag ctggctgcga
    10501 tctgctcaag caggcgtttg ctcgcgcggc gattctctct aacgagaaat tccgcggcgt
    10561 acgtctttat gtcagcgaaa accagctgaa aatcaccgcc aacaacccgg aacaggaaga
    10621 agcggaagag atcctcgacg ttacctatag tggtgcggag atggaaatcg gcttcaacgt
    10681 cagctacgtg ctggatgttc tgaacgcgct gaaatgtgaa aacgtccgca tgatgctgac
    10741 cgattcggtt tccagcgtgc agattgaaga tgccgcatca cagtcggctg cctatgttgt
    10801 catgccaatg agactgtaat gtccctcacc cgcttgttga tccgcgattt ccgcaacatt
    10861 gaaaccgcgg atctcgcttt atctcccggc tttaactttc tggtaggtgc caacggcagt
    10921 ggcaaaacca gcgtgctgga agccatctat acgctcggcc atggtcgggc gtttcgcagt
    10981 ttgcagattg gtcgcgtcat tcgccatgag caggaggcgt ttgttctcca cgggcgatta
    11041 cagggcgaag agcgcgagac agcgattggc ttaaccaaag acaaacaggg cgacagcaaa
    11101 gtccgcatcg acggtacaga cgggcataag gtcgcggaac tggcgcacct gatgccaatg
    11161 cagttgataa cgccagaagg gtttacttta ctcaacggcg gccccaaata cagaagagca
    11221 ttcctcgact ggggatgctt tcacaacgaa cccggatttt tcaccgcctg gagcaatctc
    11281 aagcgattgc tcaagcagcg caatgcggcg ctgcgccagg tgacacgtta cgaacagcta
    11341 cgcccgtggg ataaagaact gatcccgctg gcggagcaaa tcagcacctg gcgcgcggag
    11401 tatagcgccg gtatcgcggc tgatatggcc gatacttgta agcaatttct ccctgagttt
    11461 tctctgactt tctctttcca gcgcggctgg gagaaagaga cagaatatgc tgaggtgctg
    11521 gaacgtaatt ttgaacgcga tcgccagcta acctacaccg cgcatggccc gcataaagcg
    11581 gacttacgca ttcgcgccga cggtgcgccg gtggaagata ccttatcgcg tgggcagctt
    11641 aagctgttga tgtgcgcctt acgtctggcg caaggagagt tcctcacccg tgaaagcggg
    11701 cggcggtgtc tctacctgat agatgatttt gcctctgagc ttgatgatga gcgtcgcggg
    11761 ctgcttgcca gccgcttaaa agcgacgcaa tcacaggtct ttgtcagcgc gatcagtgct
    11821 gaacacgtta tagacatgtc ggacgaaaat tcgaagatgt ttaccgtgga aaagggtaaa
    11881 ataacggatt aacccaagta taaatgagcg agaaacgttg atgtcgaatt cttatgactc
    11941 ctccagtatc aaagtcctga aagggctgga tgcggtgcgt aagcgcccgg gtatgtatat
    12001 cggcgacacg gatgacggca ccggtctgca ccacatggta ttcgaggtgg tagataacgc
    12061 tatcgacgaa gcgctcgcgg gtcactgtaa agaaattatc gtcaccattc acgccgataa
    12121 ctctgtctct gtacaggatg acgggcgcgg cattccgacc ggtattcacc cggaagaggg
    12181 cgtatcggcg gcggaagtga tcatgaccgt tctgcacgca ggcggtaaat ttgacgataa
    12241 ctcctataaa gtgtccggcg gtctgcacgg cgttggtgtt tcggtagtaa acgccctgtc
    12301 gcaaaaactg gagctggtta tccagcgcga gggtaaaatt caccgtcaga tctacgaaca
    12361 cggtgtaccg caggccccgc tggcggttac cggcgagact gaaaaaaccg gcaccatggt
    12421 gcgtttctgg cctagcctcg aaactttcac caatgtgacc gagttcgaat atgaaattct
    12481 ggcgaaacgt ctgcgtgagt tgtcgttcct caactccggc gtttccattc gtctgcgcga
    12541 caagcgcgac ggcaaagaag accacttcca ctatgaaggc ggcatcaagg cgttcgttga
    12601 atatctgaac aagaacaaaa cgccgatcca cccgaatatc ttctacttct ccactgaaaa
    12661 agacggtatt ggcgtcgaag tggcgttgca gtggaacgat ggcttccagg aaaacatcta
    12721 ctgctttacc aacaacattc cgcagcgtga cggcggtact cacctggcag gcttccgtgc
    12781 ggcgatgacc cgtaccctga acgcctacat ggacaaagaa ggctacagca aaaaagccaa
    12841 agttagcgcc accggtgacg atgcgcgtga aggcctgatt gcggtcgttt ccgtgaaagt
    12901 gccggacccg aaattctcct cccagaccaa agacaaactg gtttcttctg aggtgaaatc
    12961 agcggttgaa cagcagatga acgaactgct ggcagaatac ctgctggaaa acccaaccga
    13021 cgcgaaaatc gtggttggca aaattatcga tgctgcccgt gcccgtgaag cggcgcgtcg
    13081 cgcgcgtgaa atgacccgcc gtaaaggtgc gctcgactta gcgggcctgc cgggcaaact
    13141 ggcagactgc caggaacgcg atccggcgct ttccgaactg tacctggtgg aaggggactc
    13201 cgcgggcggc tctgcgaagc aggggcgtaa ccgcaagaac caggcgattc tgccgctgaa
    13261 gggtaaaatc ctcaacgtcg agaaagcgcg cttcgataag atgctctctt ctcaggaagt
    13321 ggcgacgctt atcaccgcgc ttggctgtgg tatcggtcgt gacgagtaca acccggacaa
    13381 actgcgttat cacagcatca tcatcatgac cgatgcggac gtcgacggct cgcacattcg
    13441 tacgctgctg ttgaccttct tctatcgtca gatgccggaa atcgttgaac gcggtcacgt
    13501 ctacatcgct cagccgccgc tgtacaaagt gaagaaaggc aagcaggaac agtacattaa
    13561 agacgacgaa gcgatggatc agtaccagat ctctatcgcg ctggatggcg caacgctgca
    13621 caccaacgcc agcgcaccgg cattggctgg cgaagcgtta gagaaattgg tgtctgagta
    13681 caacgcgacg cagaaaatga tcaaccgcat ggagcgtcgt tatccgaaag caatgctgaa
    13741 agagcttatc tatcagccga cgctgacgga agccgacctc tctgatgagc agaccgttac
    13801 ccgctgggtg aacgcgctgg tcagcgaact gaacgacaaa gaacagcacg gcagccagtg
    13861 gaagtttgat gtccacacca atgccgaaca aaacctgttc gagccgattg ttcgcgtgcg
    13921 tacccacggt gtggatactg actatccgct ggatcacgag tttatcaccg gtggcgaata
    13981 tcgtcgtatc tgcacgctgg gtgagaaact gcgtggcttg ctggaagaag atgcgtttat
    14041 cgaacgtggc gagcgtcgtc agccggtagc cagcttcgag caggcgctgg actggctggt
    14101 gaaagagtcc cgtcgcggcc tctccatcca gcgttataaa ggtctgggcg agatgaaccc
    14161 ggaacagctg tgggaaacca ccatggaccc ggaaagccgt cgtatgctgc gcgttaccgt
    14221 taaggatgcg attgccgctg accagttgtt caccacgctg atgggcgacg ccgttgaacc
    14281 gcgccgtgcg tttattgaag agaacgccct gaaagcggcg aatatcgata tttaatggcg
    14341 ctaaccatgc gagcgtgcct gatgcgctac gcttatcagg cctacgaaaa tcttgcaatg
    14401 tattgaattt tcatgatttt gtaggccgga taaggcgttc acgccgcatc cggcaagaag
    14461 ctataagaaa agggcggaga tcatctccgc cctttttatt tctgcaatcc gcaattatca
    14521 tcccgcaaat ctatactttt tgggtcaaca cgtaacgctt tacaggtgat tatcatgggg
    14581 ctttttgatg aagttgtcgg tgcctttctg aaaggcgatg cggggaaata tcaggctatt
    14641 ttaagttggg ttgaggagca gggcggcatt caggtgctgc tggaaaaact gcaaagtggc
    14701 ggcttagggg ccattctctc aacctggctg agtaatcaac agggcaatca atcggttagt
    14761 ggcgagcaac tggaatcggc gctcggcaca aatgcggtgt ccgatcttgg acaaaaactt
    14821 ggcgtggata ccagtacagc ttccagttta ctggcagaac aattgccgaa gattattgat
    14881 gcgctctcac cgcaaggtga agtgtctcca caagccaata acgatctgct ttccgcaggc
    14941 atggaactgc tgaaagggaa actcttccgc taagcaaatg gggagcatgc aacccgcgtt
    15001 gcatccccct tttccctcgg gtgttgtact gatttttgag cggaatcgcg ttagcatggg
    15061 tcaggaacta atctacctgg ggaactcatg gctatcaaac tcattgctat cgatatggat
    15121 ggcacccttc tgctgcccga tcacaccatt tcacccgccg ttaaaaatgc gattgccgca
    15181 gctcgcgccc gtggcgtgaa tgtcgtgcta acgacgggtc gcccgtatgc aggtgtgcac
    15241 aactacctga aagagctgca tatggaacag ccgggcgact actgcattac ttataacggc
    15301 gcgctggtac agaaggccgc tgatggtagc accgtggcgc aaactgctct cagctatgac
    15361 gactatcgtt tcctggaaaa actctctcgc gaagtcggtt ctcatttcca cgccctggac
    15421 cgcaccacgc tgtacaccgc caaccgtgat atcagctact acacggtgca tgaatccttc
    15481 gttgccacca ttccgctggt gttctgcgaa gcggagaaaa tggaccccaa tacccagttc
    15541 ctgaaagtga tgatgattga tgaacccgcc atcctcgacc aggctatcgc gcgtattccg
    15601 caggaagtga aagagaaata taccgtgctg aaaagtgcgc cgtacttcct cgaaatcctc
    15661 gataaacgcg ttaacaaagg tacgggggtg aaatcactgg ccgacgtgtt aggtattaaa
    15721 ccggaagaaa tcatggcgat tggcgatcag gaaaacgata tcgcaatgat tgaatatgca
    15781 ggcgtcggtg tggcgatgga taacgctatt ccttcggtga aagaagtggc gaactttgtc
    15841 accaaatcca accttgaaga tggcgtggcg tttgctattg agaagtatgt gctgaattaa
    15901 tcagtggacg ggcaaacagc ataaacgcgt ttgcccgctt actgattatt ggtttactgc
    15961 atatacaacc agaaattgca gcggtgcagt aatgatgtcc agcgtaacag tcactggcag
    16021 taatgctgcg gaaacacgat ccggaatgcc tctgggtgaa tagtattcat aaaatcgcac
    16081 tttaaacgga tgatagaacg ccatcacctt tgagtaatcc atgtttttat ttttctcatg
    16141 gattgtgcct tttaggtttt ccagtgacct ggtacagtgt tgaacaccac aggcaaaacc
    16201 ataatgcgtt gccagtgcct tttcttcttc gttattccag tagtagtaaa gcgatattgt
    16261 gccggtaaat ttctttttcc ccgcatttaa aacaaatctt gcgtcatcgg caacctgaat
    16321 attgtgccgg ttaagtgctg gatcattgag catcttaacg atgtcatcgg caccatcagt
    16381 gataaggtaa tccagcgatt cccctacaaa aacatagcct tttgttccat tactatcttt
    16441 tgcctgcgat agcccagtga tggtgtctga ctgcgattga tactttcctg aactaccctt
    16501 tgcggtatgg gaaaccagag catacgagat acagcctgaa agcatcagcg ccagtgggaa
    16561 taaaccggca gccttaaaaa atcccttaaa attcaacttc atgattaatc ctttaaacga
    16621 gtagctaagc cgttgaaagc atactcttct ttccctcctg tttagcgcct gatatccctt
    16681 ttcagatttc tgcccgacgc atgtcatttt tttatgcatt gttctttttg tgatctaaat
    16741 tgtagtacaa caatataagt ttgtactaca ttacgcacac ggcaaacgcg aacgtcatca
    16801 cgctggtact acaaagttgc cgcgttatgc atcgatcggg gtaaagtaga gaagaacata
    16861 cagagcacaa ggactctcca tgactctcaa taaaaccgat cgcattgtca ttacgctggg
    16921 taaacagatc gttcacggca aatacgtacc tggctcgcca cttccggctg aggcggagct
    16981 ctgtgaagag tttgcaacct cgcgcaacat catccgtgag gtgttccgtt cgctgatggc
    17041 gaagcggctg attgaaatga aacgttatcg cggggcgttt gtggcaccgc gtaaccagtg
    17101 gaattacctc gacactgacg tactgcaatg ggtgctggaa aacgactacg acccacggct
    17161 tatcagtgcc atgagcgaag tgcgaaatct ggtggaaccg gcgattgccc gttgggcagc
    17221 agagcgcgcg acttccagcg atctggcgca gattgaatcg gcgctgaacg agatgattgc
    17281 caacaatcag gaccgcgaag cgtttaacga agcggatatt cgctaccacg aggcggtgct
    17341 gcagtcggta cataacccgg tgttacagca acttagcatt gcgatcagtt cgttgcagcg
    17401 ggcggttttt gaacgaacct ggatgggcga tgaggccaac atgccgcaaa cgctccagga
    17461 acataaggcg ctgttcgatg caatacggca tcaggacggc gatgcggcag agcaggcggc
    17521 gcttaccatg atcgccagct cgacacgaag gttaaaggaa atcacatgac agctcgctac
    17581 atcgcaattg actggggatc gaccaatctg cgcgcctggc tttatcaggg cgaccactgc
    17641 ctggagagca ggcaatcaga agcaggcgtc acgcgcctga acggaaaatc tccggctgcg
    17701 gtgttagcag aagtcacgac cgactggcgt gaagaaaata cgccagtggt gatggcagga
    17761 atggtcggca gcaatgtcgg ctggaaagtt gctccgtatt tatctgttcc tgcccgtttt
    17821 tcgtctattg gcgaacaatt aacgtctgtt ggcgacaata tctggattat tcccggatta
    17881 tgcgtctctc atgacgataa ccacaatgtg atgcgcggcg aagaaacaca attgatcggc
    17941 gcgcgagctc tggctccttc ctctctttat gttatgcccg gtacgcattg caaatgggtg
    18001 caggccgata gccagcaaat caacgatttt cgcaccgtga tgaccggtga attacatcat
    18061 ttactgttaa atcactcatt gattggcgca ggtttgccgc cgcaggaaaa ctctgccgat
    18121 gccttcgcgg ctggccttga gcgcggcctt aatgcgcccg ccatattgcc gcagcttttt
    18181 gaagttcgcg cctcgcatgt gctgggaaca cttccccgcg aacaggtcag cgaatttctc
    18241 tctggtttgt tgattggcgc agaggttgcc agtatgcgcg actatgtgac ccatcaacac
    18301 gccatcaccc ttgtcgccgg aacatcgctg accgcgcgct accagcaagc ctttcaggcg
    18361 atgggttgcg acgtgacggc ggtggcgggc gacacggcat ttcaggctgg tataaggagc
    18421 atcgctcatg cagtggcaaa ctaaacttcc gctgatcgcc attttgcgcg gcattaagcc
    18481 cgacgaggcg ctggcgcatg ttggcgcggt gattgacgcc gggttcgacg cggttgaaat
    18541 cccgctgaat tccccacaat gggagcaaag cattcccgcc atcgttgatg cgtatggcga
    18601 caaggcgttg attggcgcag gtacggtact gaaacctgaa caggtcgatg cgctcgccag
    18661 gatgggctgt cagctcatcg ttacgcccaa tatccatagt gaagtaatcc gccgtgcggt
    18721 gggctacggc atgaccgtct gccccggctg cgcgacggcg accgaagcct ttaccgcgct
    18781 cgaagcgggc gcgcaggcgc tgaaaatatt tccgtcatcg gcttttggtc cgcaatacat
    18841 caaagcgtta aaagcggtat tgccatcgga catcgcagtc tttgccgttg gcggcgtgac
    18901 gccagaaaac ctggcgcagt ggatagacgc aggttgtgct ggggcgggct taggcagcga
    18961 tctctatcgc gccgggcagt ccgtagaacg caccgcgcag caggcagcag catttgttaa
    19021 ggcgtatcga gaggcagtgc aatgaaaatc accaaaatta ctacgtatcg tttacctccc
    19081 cgctggatgt tcctgaaaat tgaaaccgat gaaggcgtgg tcggttgggg cgagcccgtg
    19141 attgaaggcc gcgcccgtac ggtggaagcg gcagttcacg agctgggtga ctatttgatt
    19201 ggtcaggatc cttcgcgcat caatgactta tggcaagtga tgtatcgcgc cggattttat
    19261 cgtggcggtc caatcctgat gagcgccatt gccgggatcg accaggcgtt atgggatatc
    19321 aaaggcaaag tgctgaatgc gccggtctgg caactgatgg gcggcctggt tcgcgacaaa
    19381 attaaagcct acagttgggt cggcggcgat cgtccggcgg atgttatcga cggcattaaa
    19441 accctgcgcg aaatcggctt cgataccttc aaactgaacg gttgtgaaga actggggcta
    19501 attgataact cccgcgcggt agatgcggca gtcaacaccg tggcacaaat tcgtgaagct
    19561 tttggcaatc agattgagtt tggtcttgat ttccacggtc gcgtcagcgc gccaatggcg
    19621 aaagtgctga ttaaagaact ggagccgtat cgcccgctgt ttattgaaga gccggtgctg
    19681 gcggaacaag ccgaatacta cccgaaattg gcggcacaaa cgcatattcc actggcggcg
    19741 ggtgagcgca tgttctcacg tttcgatttt aaacgcgtgc tggaggcagg cggtatttcg
    19801 attctgcaac cggatctctc ccatgcaggc ggtattaccg aatgctacaa aattgctgga
    19861 atggcagaag cctatgatgt gacccttgcg ccgcactgtc cgctcggacc gattgcactg
    19921 gcggcttgcc tgcatatcga ctttgtttcc tataacgcgg tacttcagga acaaagtatg
    19981 ggcattcatt acaacaaagg cgcggagtta ctcgactttg tgaaaaacaa agaagacttc
    20041 agtatggttg gcggcttctt taaaccgtta acgaaaccgg gcttaggtgt ggaaatcgac
    20101 gaagctaaag ttattgagtt cagtaaaaat gccccggact ggcgtaatcc gctctggcgt
    20161 catgaagata acagcgtagc agagtggtaa ttcctgccac gtaagcccct catcgggcac
    20221 taaaacagca atacaaaaat ataaccctct gtaaattaca gggcatggtg agcggcttcg
    20281 ctatgcccaa aatctggaga aagctcgcta tggatattcc cgttaatgca gcaaagccgg
    20341 ggcgtcggcg ttatctgacg ctggtgatga tctttattac ggtggtcatt tgttatgttg
    20401 accgcgctaa cctggccgtg gcttccgccc atattcagga agagttcggc attacgaaag
    20461 cggaaatggg ctatgtattt tcggccttcg cctggcttta tacgctatgc cagatccccg
    20521 gcggttggtt tttagatcgc gtaggttctc gcgtgactta ttttattgcg atatttggtt
    20581 ggtcagtggc gactttattc cagggctttg ccacgggatt aatgtcatta attggtctgc
    20641 gtgcgataac cggtattttc gaagcgcccg ctttcccgac caataaccgg atggtgacca
    20701 gctggttccc ggaacatgaa cgcgcttccg ccgttggttt ttatacgtct ggtcagtttg
    20761 tcggtctggc gtttttgact ccgctgctga tctggattca ggagatgttg agctggcact
    20821 gggtgttcat tgtcactggt ggtatcggca ttatctggtc gctgatttgg tttaaggttt
    20881 atcagccgcc gcgcctgacc aaaggtatca gcaaagctga actggattac attcgtgatg
    20941 gcggcggtct ggtggatggt gatgcgccgg tgaagaaaga ggcgcgtcag ccgttaacag
    21001 ccaaagactg gaaactggtg ttccatcgta aactgatcgg cgtttatctt gggcaatttg
    21061 cggtggcttc tacactgtgg tttttcttaa cctggttccc gaactattta acccaggaaa
    21121 aaggaatcac ggcgctgaaa gcaggcttta tgaccacggt gccattcctc gcggcgtttg
    21181 tcggcgtcct gctctctggc tgggtcgcgg atctgctggt acgtaagggc ttttcactgg
    21241 gctttgcgcg taaaacgccg attatctgcg gcttgctgat ctccacctgc attatgggcg
    21301 ctaactacac caacgatccg atgatgatta tgtgcctgat ggcgctggca ttcttcggca
    21361 acggttttgc ttcgattacc tggtcgctgg tctcttctct ggcaccgatg cgcctgattg
    21421 gtttaaccgg cggcgtgttt aacttcgccg gtggtctggg cggcatcacc gttccgctgg
    21481 tggtggggta cctggcgcag ggttacggtt tcgcacctgc actggtttat atctccgccg
    21541 tcgcgttgat tggcgcgctc tcttacatcc tgctggtggg cgatgtgaag cgcgttggct
    21601 aatctttcaa ctgtggaata tgcgccatac cactgcgcat aatccacttt cttaaagccg
    21661 gtgcggtcag gcatcggctt tttactatct tgccgaagag ttttaaacgc agtttgcggg
    21721 tggcgcgcca gtaagcggta ttgagcttct ctggctgctt cagtaacacc gaacgcagaa
    21781 tgtctgcgct atccagcgca tagctaatcc cctccagcga actggcgctg ataaatcctg
    21841 ccgcttcgcc aatcagaaag gcgttgtcct taccgcagac aaaatcctgc cagcgtgagg
    21901 gaaacagcac cgtgcatttt tcgcttttca ccgccttttt cgcttttcac cgccttacca
    21961 aactggaact gaaaggcgct cattttctct ttcagcgtcg tgaaacgcgt ctgaccgtct
    22021 ttcattggat aagcaccgcc aaagataaaa taaccgtctt tgctgatact ccatgaataa
    22081 cagttagtta tcgaattatc aaagatgcag gagtagaacg gcaccggatg tttctccgcg
    22141 aaccactgct ggatagcgac atatttacgg atttgatgat ccgggtagag atgtcgccgc
    22201 accatcgagt ttgcgccatc ggcaccgacc agatagcggg cggtaatatg ttgctcccag
    22261 ccgtctgcac gaaaaatgac atgccattta tcatcctcac gccagatttt tcggcacagg
    22321 ctatcgtgat aaacctcaac gctggtgggg atcagcgatt tcatccacaa gtcgaaagca
    22381 tggcgattaa tattgatata gcttcgctgg tagttacgcg tgagcgatgc ggcgacgtcg
    22441 acagttttga cgctgaaaat ctgtggattg gcgatcacat cgacaggaag cgtcagtcca
    22501 tcgcgaataa aagatcgctg cgcgtccggt gccagcagac cgccgcaagg tttgctgaaa
    22561 ccttcagtac cacactggtg ctttttatcc agcgcgatca cctgcatttt gcctgctaat
    22621 tttcgcgcca acgccgatcc tgccgggccg aggccaataa tcgccacgtc gaaatgttcc
    22681 atcttttcac tcacattcat cacgatggca aaagactatt gtctgaaagt gaagtccttg
    22741 tgaagtatga tggcgggacc cgttttgtat caggaccgcg ccatgaaaca gataaccttt
    22801 gctccccgta atcacctgct caccaatacc aatacctgga cgcccgacag ccagtggctg
    22861 gtatttgacg tgcgtccttc tggggcgtcg tttaccggcg agaccattga gcgtgtgaat
    22921 atccataccg gcgaggtcga ggttatctat cgcgcgtcac agggcgcaca cgtcggcgtg
    22981 gtgaccgttc atccgaagtc agagaaatat gttttcatcc atggcccgga aaatcctgat
    23041 gaaacatggc attacgattt ccatcatcga cgcggagtca ttgctgaagg cggcaaggtg
    23101 agcaatctcg atgcaatgga tattaccgcg ccgtatacgc caggagcgct gcgcggcggc
    23161 agccatgtgc atgtctttag cccgaacggt gaaagggtga gctttaccta taacgaccat
    23221 gtaatgcatg aactcgatcc ggcgctggat ttgcgaaacg tcggtgttgc tgcgccgttt
    23281 ggcccggtca acgtacaaaa gcagcatctg cgtgaataca gcggtagcca ctggtgcgtg
    23341 ctggtgagta aaaccacgcc cacgccgcag cctggcagcg atgaaatcaa tcgtgcttat
    23401 gaagaaggat gggtaggaaa tcacgcgctg gcgtttattg gcgacacact ttcgccaaag
    23461 ggcgagaaag tgccggagct gtttatcgtt gagttaccgc aagatgaagc tggctggaaa
    23521 gcggcaggtg atgcgccgtt aagtggaacg gaaaccaccc tgcccgcgcc accgcgcggc
    23581 gtcgtgcagc gacgtttaac ctttacccac catcgggctt atccggggtt agtcaacgtc
    23641 ccgcgccact gggtgcgctg taatccgcag ggtacgcaaa tcgcgttttt aatgcgtgat
    23701 gataacggca ttgtgcaact gtggcttatc tcgccacagg gcggcgagcc gcgccagtta
    23761 acccataaca aaacggatat tcagtctgca tttaactggc atccgtcagg agaatggttg
    23821 ggctttgtgc tggataatcg aattgcttgt gcccatgcgc aaagcggcga ggttgagtat
    23881 ttaaccgaaa accacgccaa tccaccttct gcggacgccg tggtcttctc gccggatggt
    23941 caatggctgg cgtggatgga aggtggccag ctgtggatca ccgaaactga tcgctaaatc
    24001 agttatcagg cattctggcc ggtggaataa ccgcattggt ggcttgcgcg ttggcttcgc
    24061 ttttttcaac gcgtgagcgc accgagctgt ctttgcggaa cacatcccac ggcagtaaaa
    24121 gcgtgtccat cacagcggta aacggcatat cgagaatcgc cagcgactta gtgccccagt
    24181 tggtttcatc gtcgccaatc atggtggcgc tggcgcgggt gccgggatac gttccttctt
    24241 tccctccggt atgggacatc acgctggagc atcctccgag taatgtcatt ccgctgcata
    24301 tcatgaacgc taacaacaca tttcttatca taataaattc atctgttgat cgtgggtgtt
    24361 ggcctgatga gttatagcga tcccttgctg aaaataacat catcattacg tcgcactgtg
    24421 gcggctatcg cactttaacg tttcgtgctg ccccctcagt ctatgcaata gaccataaac
    24481 tgcaaaaaaa agtccgctga taaggcttga aaagttcatt tccagaccca tttttacatc
    24541 gtagccgatg aggacgcgcc tgatgggtgt tctggctacc tgacctgtcc attgtggaag
    24601 gtcttacatt ctcgctgatt tcaggagcta ttgattatgc gtaactttga tttatccccg
    24661 ctttaccgtt ctgctattgg atttgaccgt ttgtttaacc acttagaaaa caaccagagc
    24721 cagagtaatg gcggctaccc tccgtataac gttgaactgg tagacgaaaa ccattaccgc
    24781 attgctatcg ctgtggctgg ttttgctgag agcgaactgg aaattaccgc ccaggataat
    24841 ctgctggtgg tgaaaggtgc tcacgccgac gaacaaaaag agcgcaccta tctgtaccag
    24901 ggcatcgctg aacgcaactt tgaacgcaaa ttccagttag ctgagaacat tcatgttcgt
    24961 ggtgctaacc tggtaaatgg tttgctgtat atcgatctcg aacgcgtgat tccggaagcg
    25021 aaaaaaccgc gccgtatcga aatcaactaa ttccctaagg ccgcctggcg cggcctgaca
    25081 tctccatgct cgccgtcagg gagcatatgc gaatcttcgg atttgcaggt acttactcgc
    25141 ttcttagaag gagaaatgac tatgcgtaac ttcgatttat ccccactgat gcgtcaatgg
    25201 atcggttttg acaaactggc caacgcactg caaaacgccg gtgaaagcca gagcttcccg
    25261 ccgtacaaca ttgagaaaag cgacgataac cactaccgca ttacccttgc gctggcaggt
    25321 ttccgtcagg aagatttaga gattcaactg gaaggtacgc gcctgagcgt aaaaggcacg
    25381 ccggagcagc caaaagaaga gaaaaaatgg ctgcatcaag ggcttatgaa tcagccattt
    25441 agcctgagct ttacgctggc tgaaaatatg gaagtctctg gcgcaacctt cgtaaacggt
    25501 ttactgcata ttgatttaat tcgtaatgag cctgaaccca tcgcagcgca gcgtatcgct
    25561 atcagcgaac gtcccgcgtt aaatagctaa ctagctattc tctttgcccc gccattctgg
    25621 tggggctttt tttgtcttac ttactcaaca atgcgttaaa tttcgactgt ttaagatatt
    25681 tcggcacgtt ctttcccgtg ataatgtgcg ctataaggca aatctgcttc accgattcag
    25741 ccataatccg tagcaattaa aaatgttatc tcaagagaaa tggacgatga gtgatatagc
    25801 attaacggtc agtattctgg ctttggtggc agtcgtcggt ttgtttatcg gcaatgtcaa
    25861 atttcgcggc ataggattag gtattggcgg cgtgctgttt ggtgggatca tcgtcggcca
    25921 ttttgtttct caggcgggga tgacattaag tagcgatatg ctgcatgtta ttcaggaatt
    25981 tggcctgatc ctgttcgttt atactatcgg gattcaggta gggccgggct tctttgcctc
    26041 attgcgcgtc tccggattac gcctcaacct gtttgctgtt ctgatcgtca tcatcggtgg
    26101 tctggttacc gccatcctgc ataaactgtt tgatattcca ctgccggtag tgctggggat
    26161 tttctccggt gcggttacca atacgccagc gctgggggca gggcagcaga ttttgcgcga
    26221 cttgggtaca ccaatggaaa tggtcgatca gatggggatg agttacgcga tggcgtatcc
    26281 attcggcatt tgcgggattt tgttcaccat gtggatgttg cgggttattt tccgcgtcaa
    26341 tgtcgagaca gaagctcagc agcacgagtc ttcacgcacc aatggcggcg cgctgatcaa
    26401 gactatcaat attcgcgttg agaaccctaa cctgcatgat ttagccatta aagatgtacc
    26461 gattctcaac ggcgacaaaa ttatctgctc gcgtctgaaa cgcgaagaaa ccctaaaagt
    26521 tccttcgcca gataccatta tccaactggg cgatttgctg catctggtgg ggctgccagc
    26581 ggatttacat aatgcgcaac tggtgattgg tcaggaggtc gatacttcgc tgtccacgaa
    26641 aggcactgat ttgcgcgtcg agcgtgtggt ggtcaccaat gaaaacgtgc tcggaaaacg
    26701 tattcgcgac ctgcacttta aagaacgcta tgacgttgtt atctcgcgcc tgaaccgtgc
    26761 cggggtcgaa ctggtcgcca gtggcgatat cagcctgcag ttcggcgata tcctcaatct
    26821 ggtggggcgt ccgtccgcaa ttgatgccgt tgccaatgtg ctggggaatg cgcagcaaaa
    26881 actgcaacag gttcagatgc tgccagtgtt tattggcatc gggcttggcg tattgttagg
    26941 ttctattccc gtctttgtgc ctgggttccc ggccgcgttg aaactggggc tggcgggcgg
    27001 tccgctgatt atggcgttga tcctcgggcg tatcggcagt atcggcaagc tgtactggtt
    27061 tatgccgcca agtgccaacc tcgcgctgcg ggagctgggg atcgtgctgt tcctctcggt
    27121 cgttggtctg aaatctggtg gagattttgt gaataccctg gtcaatggcg aagggctaag
    27181 ctggattggt tatggtgccc tgatcaccgc cgttccgctg attactgttg gcattctggc
    27241 gcggatgtta gccaaaatga attacctgac catgtgcggg atgctggcag gttccatgac
    27301 cgatcctccg gcgctggcgt ttgctaataa tcttcatcca accagcggtg cggcggcgct
    27361 ctcttacgcc actgtctatc cgttagtgat gttcctgcgc attatcaccc cccaattact
    27421 ggcggtgctc ttctggagta tcggttaact cttttccggg tggagtcgcc gtaaatggta
    27481 ctccacctgg tattcgctgg catttctgaa catcactgaa taattcaaaa actccccgct
    27541 ctcgctatat gacaacgagg tgatccgcag cagtggcgtg tgttccgcta ccttcatttg
    27601 ccgcgccagc agtctatcgg cgagcactgg cgtcaggctt tcataattac cgccaatcaa
    27661 aatcccgcac tcttgttcaa tatattcaaa cttcgaccct tccagatgtt gcagcgaaag
    27721 attacggaac agttttaccg gcatatagct gtcttccagc atcagcggtt tcccttccac
    27781 aaaacgaacg cgacgggaga agtagatctg ctcgttgatt tgaatccgta actggctggc
    27841 aatggccgga ggcgcaggca tgatttcaaa aatcagcacc tggctggtga ccgtttttcc
    27901 ctgccgtttt aacacctcca ccagcccggt caggctggcg gtttgatgca gtacatcttt
    27961 gcgcaccagg taagtgccgc tgccgtggcg gcggaccacc aatccccacg ctaccagcag
    28021 gtcaatggct ttacggatgg tcatccgcga taccgcaaat tcttccgcca actttttttc
    28081 accgggaaga aggctattta gcgtgaaatc tgcggaatta agtcgaattc ttaaccgctc
    28141 cgcaatgctt ttgtagatca tctctagatc tcttttttgc ctgcaagcac gggtaatgcg
    28201 cccacaagtt ctgcttaatc gattgaaaaa acatcttttt tttaaagatg tgttcgatgc
    28261 tgtgtacctg tgctcacaga tgtctacttt ttcgcgaaaa cgtagatctc taccgcccaa
    28321 cgaaaagcat gaaagcgatc acgaatccca ttgggtcgtc atgttctgcc gatcgcatct
    28381 tcctatcctc gctccaggcc tgccgcataa ccaatcaggc ttcctactta cagaattgag
    28441 aaaagaggat gtggaaatgc tcagtcaaat tcaacgcttt ggcggcgcga tgttcacgcc
    28501 agtgctgctg tttcccttcg ccgggattgt ggtgggtctt gccatcttgc tgcaaaaccc
    28561 gatgtttgtc ggggaatcac tgaccgatcc gaacagttta ttcgcgcaaa tcgtacacat
    28621 tattgaagag ggcggttgga cggtattccg taatatgccg ctgatttttg ctgtcggttt
    28681 acccattggc cttgctaagc aagcgcaggg gcgtgcttgt ctggcggtga tggtgagttt
    28741 cctgacctgg aactatttca tcaacgcgat gggaatgacc tggggaagct acttcggcgt
    28801 cgatttcact caggacgcgg tggcaggtag cggtctgaca atgatggccg ggattaaaac
    28861 cctcgatacc agcattatcg gcgcaattat catttccggc attgtgacgg cgctgcataa
    28921 ccgtctgttc gataaaaaac tgccggtttt tctcggcatt ttccagggga cgtcttatgt
    28981 ggtgattatc gccttcctgg tgatgatccc ctgtgcctgg ctgacgttgc tcggctggcc
    29041 aaaagtacaa atggggattg aatctctgca agcgttcctg cgttcggcgg gtgcacttgg
    29101 ggtctgggtt tacaccttcc tcgaacgtat tctgatccca accggtttac accacttcat
    29161 ctacggacag tttatctttg gtccggcagc tgttgaaggc ggcattcaga tgtactgggc
    29221 gcagcatctg caagagttca gtttgagcgc cgagccgctg aaatcgttgt tcccggaagg
    29281 cggttttgcc ctgcacggta actcaaaaat ctttggtgcc gtgggcattt ctttagcgat
    29341 gtacttcact gccgcaccgg aaaatcgggt aaaagtggcg ggcttgctga ttcccgcaac
    29401 cttaaccgcc atgctggtgg gaattaccga accgctggaa tttaccttcc tgttcatttc
    29461 accgttgctg tttgcggtac acgctgtgct tgcggcctca atgtcgaccg tgatgtatct
    29521 ctttggtgtg gtgggcaaca tgggcggagg tctgattgac caggttttac cgcaaaactg
    29581 gatcccgatg ttcagcaacc acgcggatat gatgctgacc caaatcgcca ttgggttgtg
    29641 ctttaccctg ctgtacttcg tggttttccg cacactgatt ctgcagttca acatgtgcac
    29701 gccgggacgt gaagatgcgg aagtgaaact ctactcaaaa gccgaataca aagcctcgcg
    29761 aggccaaacc accgcggcag agccaaaaaa agagctggat caggctgccg gtatcctgca
    29821 agccctgggc ggggtcggca atatctccag cattaacaat tgtgcgacgc gtctacgcat
    29881 tgcactgcat gacatgtcac aaacgctgga tgacgaagtc tttaaaaagc tgggagcgca
    29941 cggcgtcttc cgtagtggcg atgccattca ggtgatcatt ggtctgcatg tatcccagct
    30001 gcgtgaacag ctcgatagct taattaattc tcatcaatca gcagaaaatg ttgccattac
    30061 ggaggcagta taatgaccaa attctcagtg gttgtcgcag gcggtggaag cacctttacg
    30121 ccaggcatcg tgttgatgct cctggcgaat caggagcgtt tcccgcttcg tgcactgaaa
    30181 ttttatgata acgatggcgc gcggcaggaa gtgattgccg aagcctgtaa agtcatcctt
    30241 aaagagaaag cgccggacat tgcgtttagt tacaccaccg atcctgaagt ggcgttcagc
    30301 gacgttgatt ttgtcatggc gcacatccgc gtcggcaaat acccgatgcg cgaactggat
    30361 gaaaaaatcc cactgcgcca cggtgttgtt ggtcaggaaa cttgcggacc cggcggaata
    30421 gcgtacggca tgcgttccat tggcggcgtc ctggaactgg tggattatat ggaaaaatat
    30481 tcaccaaacg cctggatgct caactactcc aacccggcag caattgtcgc agaagccacg
    30541 cgtcgtctgc gcccgaatgc gaaaatcctc aacatctgtg acatgccaat cggtattgaa
    30601 agccggatgg cgcaaattgt tgggctgcaa gatcgcaaac agatgcgcgt gcgctactac
    30661 ggcctgaacc actggtggtc ggcgatatcc cgcagtttca gaaagggtta atgagtcagc
    30721 aagtggcggt ggaaaaactg gtcgtggacg cctgggaaca gcgttcatat cagcacctgt
    30781 ggtaggcgat tacgttgtcg aaaacggtac cgagcgcctc ggtcgccaaa gctattctgg
    30841 atgagttgct ggaggccaac aaagcgtact ggccagagtt acgttaattg tgtggactgg
    30901 catccgttgg gtgctggtcc gtattcttta gcacggctga cggcgcataa ccaaatttat
    30961 gtttaaaggc tttgctgaaa tgaaaggagt cgaagaagtt gagcgtatca gcgacttcgc
    31021 caaccgagtt tccttgttgc tttaatagcg atagcgccag atccagacgc gcatcgaggt
    31081 aatattcttt cggcgtcttg ccggtatagc gtaaaaacag acgacgcaac caggcttcgc
    31141 tacaggggat cgtggcagcc atatcagcta cgctccagcg ttgttgcaga ctggcatgta
    31201 aagtggcaat taatttttca atttgccgcc gttgtggatc ttttttacca tctgcgtaca
    31261 taagacaaat ccactgataa ataattttcg tcagaaaagc gactgccaga ttatttttta
    31321 ttgcttctgg tgaagttatt aactcagcaa cttcggtgag ttcctgatta taaatttcgc
    31381 cattataaat aacgctttgc tgaccaacgg gaatatccat catactggtg ggggtaaatt
    31441 ccatccagta ctgttcccag actaaacctt cacagtgata agagtgaata tccattggct
    31501 ttaagaatat aatacagttg ccatgcagtg ttatttgttc gccattcttt aaaagaattt
    31561 tcccacatcc ttgtacggta tacaccgcaa cccatgagtt ggcgataagc ggtttcttct
    31621 tgtcccgcgg ccagatcacc cggtaatgct cgtcggcgta ggtatgccag agcgagatca
    31681 gagaaattcc gctggagatc acattttcgt taatcaataa gggaatattg tacggagttt
    31741 cgttttttac atctgaactt tgcaattttc cattcatttt aatatccctc cggcgaatta
    31801 tgctcacaac ctgaacttaa cagaaaacaa tccgttcagg gatacgacgt ttgtatttta
    31861 agaatctgac tgcctgaccc gacgcatttt acctctccct atattcatgc gtccgggaca
    31921 taaacataaa taagggctat gagatgaatt cgttacaaat cttgagtttt gtcggtttta
    31981 cgctgctggt ggcggtgatc acctggtgga aggtccgcaa aacagatacc ggatcgcaac
    32041 aaggctattt tcttgccgga cgttcactaa aagcgccggt tattgccgct tcgttaatgc
    32101 taaccaacct ttccacggaa caactggtcg gcctttccgg gcaggcctac aaaagcggca
    32161 tgtcggtgat gggctgggaa gtgacttcag cggtgacgct gatcttcctc gcgctaatct
    32221 ttttaccgcg ctatctgaag cgcggcattg ccaccatccc cgattttctg gaggaacgtt
    32281 atgataaaac gacgcgtatt atcatcgact tctgcttcct cattgccacc ggcgtctgct
    32341 ttctgccgat tgttctctac tccggcgcgt tggcgctcaa cagcctgttt cacgtcgggg
    32401 aatcgctaca gatttctcac ggtgcggcta tctggctact ggtaattttg cttggtctgg
    32461 cgggaatttt gtatgcggtg atcggcggac tgcgcgcaat ggcagtggcg gactccatca
    32521 acggtattgg gctggttatc ggcgggttga tggtgccggt atttggcctg atcgcgatgg
    32581 gcaagggcag ctttatgcag ggcattgagc aactcaccac cgttcacgcc gagaaattaa
    32641 actcaatcgg tggcccgacc gatcccttgc cgattggcgc ggcatttacc ggtttgattc
    32701 tggtgaacac cttttactgg tgtacaaatc agggcatcgt gcaacgcacg ctggcgtcaa
    32761 aaagcctggc ggaagggcaa aagggggcgc tgttaacggc ggtgctgaaa atgctcgacc
    32821 cgctggtact ggtgctgcca gggttgattg cgtttcatct gtatcaggat ttaccgaaag
    32881 ccgacatggc ctacccgacg ctggtcaata acgttctgcc agtgccaatg gtgggtttct
    32941 tcggcgcggt gttatttggt gcggtgatca gtaccttcaa cggctttctg aatagcgcca
    33001 gtacgttatt cagtatgggt atttaccgtc gcatcattaa ccagaatgcc gagccgcagc
    33061 agctggtcac cgtcgggcgc aaatttggtt tctttatcgc tatcgtttcg gtgctggtcg
    33121 cgccgtggat cgccaacgcg ccgcaggggc tgtatagctg gatgaaacag ctcaacggca
    33181 tttacaacgt gccgctggtt accatcatca ttatgggctt tttcttcccg cgcatcccgg
    33241 cgctggcggc aaaagtggcg atggggattg gcataatcag ctacatcacc atcaactatc
    33301 tggtgaagtt cgacttccat ttcctctatg tgctggcctg tacgttctgc atcaacgtgg
    33361 tcgtgatgct ggtgatcggt tttatcaaac cgcgcgccac gccgttcacc ttcaaagatg
    33421 cgtttgcggt agacatgaaa ccgtggagaa acgtcaagat cgcgtcaatt ggcatcctgt
    33481 tcgcgatgat tggcgtctat gccgggctgg ctgaattcgg cggctacggt acgcgctggt
    33541 tagcgatgat cagttatttc attgccgccg tagtgattgt ctacctgatt tttgacagct
    33601 ggcggcatcg tcacgaccca gccgtaacct ttactcccga cgcgaaggat agcctatgaa
    33661 acgccccaat tttctgttca tcatgaccga tacccaggcc accaatatgg tcggttgcta
    33721 tagcggtaaa ccgctgaata cgcaaaatat tgatagtctg gcggcggaag gtattcgctt
    33781 taattccgcc tacacctgtt caccggtttg tacgcctgca cgcgccggac tatttaccgg
    33841 tatctacgct aaccagtccg gcccgtggac caacaacgtc gcgccaggca aaaacatctc
    33901 cactatgggg cgctacttta aggatgccgg ctatcacacc tgttacatcg gcaaatggca
    33961 tctcgacggt catgactatt tcggcactgg cgagtgtccg ccggagtggg acgctgatta
    34021 ctggttcgat ggggcgaact accttagcga actgacggaa aaagagatca gcctgtggcg
    34081 caatggccta aacagcgttg aggatttaca ggcgaaccat atcgacgaaa ccttcacctg
    34141 ggcgcaccgc atcagcaatc gggcggtaga ttttctgcaa cagcccgcgc gcgccgagga
    34201 acccttcctg atggtggttt cgtatgatga gccgcatcac ccgttcacct gtccggtgga
    34261 gtatttagag aaatacgctg atttttacta cgatctgggc gagaaagctc aggatgacct
    34321 ggcgaacaaa ccggaacatc accgcttatg ggcgcaggcg atgccatcgc cagtcggtga
    34381 tgacgggctt tatcaccatc cgctctattt tgcctgtaat gactttgttg atgaccaaat
    34441 cggacgggtc atcaatgcct taacgccaga gcaacgtgaa aatacgtggg tcatttatac
    34501 ttccgatcac ggcgaaatga tgggcgcaca taagctgatc agtaaaggag cggcgatgta
    34561 tgacgacatc acccgtattc cgctgatcat ccgttcgccg caaggggagc ggcgacaggt
    34621 cgatacgcca gtcagtcata tcgatttact gccgacaatg atggcgctgg cagatattga
    34681 aaaaccagag attctgccgg gggaaaatat ccttgccgtg aaagagccac gcggtgtaat
    34741 ggtggaattt aaccgctacg agattgagca tgacagcttt ggcggtttta ttccggtgcg
    34801 ttgctgggtg acggatgact ttaaactggt actcaacctc ttcaccagtg atgaacttta
    34861 cgatcgccgt aatgacccaa atgaaatgca taacctgatc gatgatatcc gttttgcaga
    34921 cgttcgcagc aaaatgcatg acgccttatt ggattacatg gacaaaattc gcgatccgtt
    34981 ccgcagttac caatggagcc tgcgtccgtg gcgtaaagat gcactgccgc gctggatggg
    35041 ggcatttcgt ccacgcccac aagatggcta ttcgccggtg gtacgtgact atgacaccgg
    35101 cctaccgacg caaggagtga aagtggaaga gaaaaaacag aagttctgac gataatgccc
    35161 gctgatgaac acccggcggg cattattcag gctgcttaaa ccagtgaacc gccggtcagg
    35221 aaggaaaaaa tcaggaatat cagcaaaatg ctgcacacga tgccgatacc aaaaagcaag
    35281 gtatggaaag ccagcgtccc gccgccaaaa accagcgcgc cgcgaatgcc ccagcgattt
    35341 tccttacgca taccgccaat aaatgcgcaa agtagcgcag caatggccag tgcgataccg
    35401 ctttgatcga ggattttatc gacatctacg tcctcttttt tctccacggt ggtaattttt
    35461 tcgcctttta ttccggcgag aagacctctt ttaatttcag ccgttttgtc cgccacgatg
    35521 ctttcaaacg ttggcggtgg tgcggaaaaa gggccgaatg aaaagtggat aatccccatc
    35581 attaaagcga tggcaccgaa cagcatgccc aatgaactga ttttattttg agctatgata
    35641 cccatctgac ctccttgtag cggatggcga aagcatatca ggaaacgggc ggacgaaatt
    35701 cttaaaaaaa aaccgctaaa ggtagagcgt tagtgaaatt gcaaaggagc aacaatgaaa
    35761 atttcccgcc ttggagaagc gcccgattac cgcttctcgc tggctaatga gcgtactttt
    35821 ctggcgtgga tccgtaccgc actaggattt ctggcggcgg gggttgggct tgatcaactt
    35881 gcgccagatt tcgccacgcc cgtcattcgt gaactgctgg cgttgttgtt gtgtctgttt
    35941 tccggcgggt tagcgatgta tggctacctg cgctggttac gcaatgaaaa agcgatgcgt
    36001 ctgaaagagg acctgcccta caccaacagc ttattaatca ttagcttaat attgatggtc
    36061 gtggcggtga tcgttatggg actggtgttg tatgccggat agccgcaaag ccagacgcat
    36121 tgccgaccca ggactgcagc cggagcgcac atcactggcg tggtttcgta ccatgctggg
    36181 ctacggcgcg ttgatggcgt tggctatcaa acacaactgg caccaggcgg gcatgttatt
    36241 ctggatttcg attggcattc tcgccatcgt ggcgctgatc ctctggcact acacccgtaa
    36301 tcgcaattta atggatgtca cgaatagcga tttttcccaa tttcacgtag tccgtgacaa
    36361 atttttgatc tccctcgcgg tgttatctct cgcaatactg tttgctgtaa cgcatataca
    36421 tcaacttatc gtatttattg agagagtcgc atgacaggaa gtcaggtcat tgatgctgaa
    36481 gaggacaggc ataaactggt tgtagagtac aaggatgctc tgcaacctgc cgatttttat
    36541 cacaatttca aacagcgggg catccgctcc gtacagctca ttccctatct tgaatttgat
    36601 gaccggggcg atctgacggc cgcttcggtg acagcagagc tgtgggggaa atttttaatt
    36661 gccctgttcg agtgttgggt gcgggcagat atcagtcgta tctcgattga gctttttgac
    36721 gccaccttac aaaaatggtg tggcagcgaa aacccgcaac cgcgccgtga ctgccaggcg
    36781 tgcgactggc atcgcctgtg cccgcacgct cgccaggaga cgccagacag cgtgctttgt
    36841 gcgggctatc aggcgtttta ttcttactcc gcaccgcata tgcgggtgat gcgtgatctg
    36901 ataaagcagc atcgttctcc catggagctg atgacgatgc tgcggtgacg tgcgcttaaa
    36961 cgggctgccc ctgatgcgac atccgcgtcg ccagcggcag ccagcacagc acgatcaaca
    37021 atcccattaa ggtcatcaac aaccccaggc tgccctgacc ggtttgcggc aacatcgcag
    37081 agagcgacgc cagcacgccg gaaccaatgt tttgcagacc gccgaccagc gcgccagccg
    37141 tgcccgccag gaaggggaac ggctccatcg cgccgctggt cgccagcgga aacagcatcc
    37201 cggcaccgaa aaagaacagc gcggcgggaa cgagcagcgt ccagacattc atcacgccaa
    37261 accagtcggg gatccacatc agcaagccag ccagcaggca gcagataacc gactgccaca
    37321 ttaacgtgga gaagcgttta ttgggacgtc cggcaaacca tgcgccaaaa aacgctgccg
    37381 gaatcggcag aataaacaaa atactgaccg tcatactgct cagccctaac accgcgccca
    37441 tcagcacgcc ggagcaggct tcaaaggcgg caatcccggc cagaccgcca atcagcatca
    37501 gcaaataaca gttaaaaccg ctgttaccga aaagcgtttt ataactggta agcaggcgcg
    37561 tgcgcggtgc atcgaccgga cgcgtttccg gcatccagcg ggccatactg aaggtcacac
    37621 cagcacaaag aaccaacaaa aagagataac aggcgcgcca gttccacatc gtatccagca
    37681 gaccgccgat tagcggtgcg agcaacggac tgacgagaat ccccatgttt aacaggctgt
    37741 tagcatggcg caactgtgtc cgttcatata aatctcgcgg caaagtacgc gccattacgc
    37801 cgccaacgcc ggtgcccatc ccctgcatcg cgctggcggc aatcaacacc gtcaaactgg
    37861 aggtcg
//
LOCUS       DAMZET010000026        36688 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.026, whole genome shotgun sequence.
ACCESSION   DAMZET010000026 DAMZET010000000
VERSION     DAMZET010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.026"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..362
                     /locus_tag="POB34_001422"
     CDS             <1..362
                     /locus_tag="POB34_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDI5918403.1"
                     /translation="SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSV
                     PYPMLDMIARSMERLGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDE
                     GIEKTAAWLRDHGKLPR"
     gene            complement(359..1189)
                     /gene="amiD"
                     /locus_tag="POB34_001423"
     CDS             complement(359..1189)
                     /gene="amiD"
                     /locus_tag="POB34_001423"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HDI5918404.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(1186..1509)
                     /locus_tag="POB34_001424"
     CDS             complement(1186..1509)
                     /locus_tag="POB34_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDI5918405.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            1635..2150
                     /locus_tag="POB34_001425"
     CDS             1635..2150
                     /locus_tag="POB34_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDI5918406.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKKGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            2368..3096
                     /gene="artP"
                     /locus_tag="POB34_001426"
     CDS             2368..3096
                     /gene="artP"
                     /locus_tag="POB34_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HDI5918407.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            3114..3845
                     /gene="artJ"
                     /locus_tag="POB34_001427"
     CDS             3114..3845
                     /gene="artJ"
                     /locus_tag="POB34_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI5918408.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            3852..4568
                     /gene="artQ"
                     /locus_tag="POB34_001428"
     CDS             3852..4568
                     /gene="artQ"
                     /locus_tag="POB34_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HDI5918409.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            4568..5236
                     /gene="artM"
                     /locus_tag="POB34_001429"
     CDS             4568..5236
                     /gene="artM"
                     /locus_tag="POB34_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HDI5918410.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            5528..6259
                     /gene="artJ"
                     /locus_tag="POB34_001430"
     CDS             5528..6259
                     /gene="artJ"
                     /locus_tag="POB34_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HDI5918411.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(6434..7561)
                     /gene="rlmC"
                     /locus_tag="POB34_001431"
     CDS             complement(6434..7561)
                     /gene="rlmC"
                     /locus_tag="POB34_001431"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HDI5918412.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(7602..8090)
                     /locus_tag="POB34_001432"
     CDS             complement(7602..8090)
                     /locus_tag="POB34_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HDI5918413.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(8150..8995)
                     /gene="potI"
                     /locus_tag="POB34_001433"
     CDS             complement(8150..8995)
                     /gene="potI"
                     /locus_tag="POB34_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HDI5918414.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVIMGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(8992..9945)
                     /gene="potH"
                     /locus_tag="POB34_001434"
     CDS             complement(8992..9945)
                     /gene="potH"
                     /locus_tag="POB34_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HDI5918415.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLSEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(9955..11088)
                     /gene="potG"
                     /locus_tag="POB34_001435"
     CDS             complement(9955..11088)
                     /gene="potG"
                     /locus_tag="POB34_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HDI5918416.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(11183..12295)
                     /gene="potF"
                     /locus_tag="POB34_001436"
     CDS             complement(11183..12295)
                     /gene="potF"
                     /locus_tag="POB34_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HDI5918417.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHVYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(12646..13122)
                     /locus_tag="POB34_001437"
     CDS             complement(12646..13122)
                     /locus_tag="POB34_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HDI5918418.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(13210..14112)
                     /gene="rimK"
                     /locus_tag="POB34_001438"
     CDS             complement(13210..14112)
                     /gene="rimK"
                     /locus_tag="POB34_001438"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HDI5918419.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(14173..14895)
                     /gene="nfsA"
                     /locus_tag="POB34_001439"
     CDS             complement(14173..14895)
                     /gene="nfsA"
                     /locus_tag="POB34_001439"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HDI5918420.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(14879..15166)
                     /locus_tag="POB34_001440"
     CDS             complement(14879..15166)
                     /locus_tag="POB34_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HDI5918421.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            15326..15583
                     /gene="grxA"
                     /locus_tag="POB34_001441"
     CDS             15326..15583
                     /gene="grxA"
                     /locus_tag="POB34_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HDI5918422.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(15613..15990)
                     /locus_tag="POB34_001442"
     CDS             complement(15613..15990)
                     /locus_tag="POB34_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HDI5918423.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            16260..17945
                     /locus_tag="POB34_001443"
     CDS             16260..17945
                     /locus_tag="POB34_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HDI5918424.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(18181..18399)
                     /locus_tag="POB34_001444"
     CDS             complement(18181..18399)
                     /locus_tag="POB34_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HDI5918425.1"
                     /translation="MMICPLCGSAAHTRSSFQVSSLTKERYNQCQNINCSHTFVTHET
                     FVRSIATPKESNPVQPHPMKSGQVALSL"
     gene            complement(18490..19590)
                     /locus_tag="POB34_001445"
     CDS             complement(18490..19590)
                     /locus_tag="POB34_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HDI5918426.1"
                     /translation="MNFSSELLNKGNKTPAFSISIEGKDITTVLDNRLMSLTLTDNRG
                     FEADQIDLELDDADGKIVLPRRGAVITLALGWKGQPLFPKGAFTVDEIEHTGAPDRLT
                     IRARSADFRETLNTRREKSWHKTTVGELVKEIAARHKLKMALGKDLSDKPVEHIDQTN
                     ESDGSFLMRLARQYGAIASVKNGNLLFIRQGQGKSASGKPLPVISITRKDGDSHRFTL
                     ADRGAYTGVIASWLHTREPAKKESTTVKRKRRTKKQKKEPEAKQGDYLVGTDENVLVL
                     NRTYANRSNAERAAKMQWERLQRGVASFSLQLAEGRADLYTEMPVKVSGFKQPIDDAE
                     WTITTLTHTVSPDNGFTTSLELEVRIDDFEME"
     gene            complement(19587..20072)
                     /locus_tag="POB34_001446"
     CDS             complement(19587..20072)
                     /locus_tag="POB34_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI5918427.1"
                     /translation="MMMVLGLYVFMLRTVPYQELQYQRSWRHAANSRVNRRPSTQFLG
                     PDNDMLTLSGVLMPEITGGRLSLLALEQMAEQGKAWPLIEGSGTIYGMYVIEGLNQTK
                     TEFFRDGMPRRIEFTLSLKRVDESLSDMFGDLSAQLNNLQDTATSALSDISKTVGGLL
                     S"
     gene            complement(20069..20500)
                     /locus_tag="POB34_001447"
                     /pseudo
     CDS             complement(20069..20500)
                     /locus_tag="POB34_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282780.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            20497..21000
                     /locus_tag="POB34_001448"
                     /pseudo
     CDS             20497..21000
                     /locus_tag="POB34_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520343.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(21041..21658)
                     /locus_tag="POB34_001449"
     CDS             complement(21041..21658)
                     /locus_tag="POB34_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HDI5918428.1"
                     /translation="MHKTFLSYHHANEQDLKDEIIDLFGGESFIDKSVSDGDISTTNT
                     DETIMRTIREDFLKDSTVTVVLIGTETKNRPFVNTEIQASLWGENYNGLIGVIRDEIY
                     DSVFTPAVCADPSCGCSISLRTPGLGYDYYLPYLVRKNHVYEKTVPHYNDSDVYCALV
                     KYSVFIKNPEYYIDQAFNKRKLMEPAAKRNPSDVPAIRSSSIFNW"
     gene            complement(21658..22389)
                     /locus_tag="POB34_001450"
     CDS             complement(21658..22389)
                     /locus_tag="POB34_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918429.1"
                     /translation="MYRLWVLWSCIFVLLLIIMGSIPFSPFEKWWFIVNFMSNVSIIR
                     AFLSVLILFLVAYILLVGAFHQRYSLRLEQLSFGGINILLNKSDLLFKKSVKNYLDTK
                     RTLFKFDPNYDSIEEVLNSYYECYKFIRDEMKLLDVKKKRDKKLYLLSNEILKTLNSF
                     LTKHQNNYRRWHKYVSDHDKVTTKDRDPNGEFISLPYHLTPISIIQKHYYHFSQVLEG
                     FKEVNDFFNDKVVKEFDINVDKWSE"
     gene            complement(22765..22998)
                     /locus_tag="POB34_001451"
     CDS             complement(22765..22998)
                     /locus_tag="POB34_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HDI5918430.1"
                     /translation="MRIEIMIDKEQKISQSTLDALESELYRNLRPLYPKTVIRIRKGS
                     SNGVELTGLQLDEERKRVMKIMQKVWEDDSWLH"
     gene            complement(23009..23197)
                     /locus_tag="POB34_001452"
     CDS             complement(23009..23197)
                     /locus_tag="POB34_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918431.1"
                     /translation="MQDYFLESLKLQRIDFFLKLVAASECSDEEKGLALQWVSELTDE
                     LMAKIRTHEYNRSMDVIN"
     gene            complement(23351..25723)
                     /locus_tag="POB34_001453"
     CDS             complement(23351..25723)
                     /locus_tag="POB34_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HDI5918432.1"
                     /translation="MNYSYSWNAEKKAINPYVETEEQSSVSALSNLIALYAADNEQEQ
                     LRREALSDQVWERYFFNESRDPVQREMEQDKLISRAKLAHEQQRFNPDMVILADVNAQ
                     PSHISKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLEHVRDCQLSTSFRFMASHE
                     GLDGLLILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYVTDDVKPEEIRKTWEKVAA
                     ETLHLDVIPPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWYRKLWKMRCEWREEQ
                     LRAVCLVSKKASPYVSYEAVMHKREQRRKSLEFFRSHELVNEDGDTLDMEDVVNASSS
                     NPAHRRNEMMACVKGLELIAEMRGDCAVFYTITCPSRFHSTLNNGRPNPTWTNATVRQ
                     SSDYLVGMFAAFRKAMHKAGLRWYGVRVAEPHHDGTVHWHLLCFMRKKDRRAITALLR
                     KFAIREDREELGNNTGPRFKSELINPRKGTPTSYIAKYISKNIDGRGLAGEISKETGK
                     SLRDNAEYVNAWASLHRVQQFRFFGIPGRQAYRELRLLAGQAARQQGDKKAGARVLDN
                     PRLDAILAAADAGCFATYIMKQGGVLVPRKYHLIRTAYEINEEPTAYGDHGIRIYGIW
                     SPIAEGKICTHAVKWKMVRKAVDVQEAAADQGACAPWTRGNNCPLAENLNQQEKDKSA
                     DGDTRTDITRMDDKELHDYLHSMSKKDRRELAVRLRLVKPKRCKDYKQRITDHQHQQL
                     VYELKSRGFDGSEKEVDLLLRGGSIPSGAGLRIFYRNQRLKEDDKWRNLY"
     gene            complement(25723..26544)
                     /locus_tag="POB34_001454"
     CDS             complement(25723..26544)
                     /locus_tag="POB34_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918433.1"
                     /translation="MHHGIHHFHGTPVWGSAGDVHRIAVSGAGAFVSYVRPDQIAASI
                     QHAQVVGIDNGAFSAWVRGLKINWSDFYKWLLNYYHHPKVAFFVIPDVVDGGERDNDA
                     LINEVPKMFYGKATPVWHLHESIDRLIELCREWPRVCFGSSGEYAAIRTAHWHRRMQD
                     AFEAIYCRHNFKTAVHGLRMLDGRVLGNYPLATADSTNLACNVPKFNSKYPELTRAIQ
                     EAEYSRNLTEKELKAVILKNRCAILKGAIEAVRPPSVSDWLSNGLQPSQLELEIA"
     gene            complement(26550..27404)
                     /locus_tag="POB34_001455"
     CDS             complement(26550..27404)
                     /locus_tag="POB34_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HDI5918434.1"
                     /translation="MSTILKWAGNKTAIMSELKKHLPAGPRLVEPFAGSCAVMMETDY
                     PSYLVADINPDLINLYKKVAADCEAFISRARVLFEEANREVAYYNIRQEFNYSTEITD
                     FMKAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNYKNPYFPEKEIRAFAEKAQRATFIC
                     ASFDETLAMLKVGDVVYCDPPYDGTFSGYHTDGFTEDDQYHLASILEHRSSEGHPVIV
                     SNSNTSLTRSLYRNFTHHYIRAKRSIGVAAGEGKFATEMIATKSANWFSADFSRGRDS
                     TVIFGVQV"
     gene            complement(27401..27628)
                     /locus_tag="POB34_001456"
     CDS             complement(27401..27628)
                     /locus_tag="POB34_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HDI5918435.1"
                     /translation="MADAMDLVQQRVEEERQRHIRAARAKTPGVSRVLCVECEAPIPP
                     ARRRAIPGVQLCITCQEIAELKGKHYNGGAV"
     gene            complement(27628..27861)
                     /locus_tag="POB34_001457"
     CDS             complement(27628..27861)
                     /locus_tag="POB34_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HDI5918436.1"
                     /translation="MRNIETLTTKTGPDDAGLNLLLTEARLEERRARAEAMAARLDSL
                     ACHITSRQLNHVEAAELLRVTAEAIQNEAQEIH"
     gene            complement(27929..28270)
                     /locus_tag="POB34_001458"
     CDS             complement(27929..28270)
                     /locus_tag="POB34_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5347 domain-containing protein"
                     /protein_id="HDI5918437.1"
                     /translation="MAIEGAAATVPLRPGERLNGLNHIAELRAKVFGLNIESELERFI
                     KDMRDPRDINNEQNKRALAAIFFMAKIPAERHSISINELTTDEKRELIKAMNHFRAVV
                     SLFPRRLTMPN"
     gene            complement(28234..28434)
                     /locus_tag="POB34_001459"
     CDS             complement(28234..28434)
                     /locus_tag="POB34_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2724 domain-containing protein"
                     /protein_id="HDI5918438.1"
                     /translation="MLTKEPSFASLLVKQSPAMHYGHGWIMGEDGKRWHPCRSQDELL
                     AELSTKKRGNKWLLKALRRLFH"
     gene            complement(28442..28951)
                     /locus_tag="POB34_001460"
     CDS             complement(28442..28951)
                     /locus_tag="POB34_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory CII family protein"
                     /protein_id="HDI5918439.1"
                     /translation="MFDYQVSKHPHFDEACRAFALRHNLVQLAERAGMNVQILRNKLN
                     PAQPHLLTAPEIWLLTDLTEDSTLVDGFLAQIHCLPCVPINEVAKEKLPHYVMSATAE
                     IGRVAAGAVSGDVKTSAGRRDAISSINSVTRLMALAAVSLQARLQANPAMASAVDTVT
                     GLGASFGLL"
     gene            complement(28984..29205)
                     /locus_tag="POB34_001461"
     CDS             complement(28984..29205)
                     /locus_tag="POB34_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HDI5918440.1"
                     /translation="MRPNISITLTTPHVTIERYSELTGLSIDTINDMLADGRLIRHRL
                     RKDKKREKVMINIAAMTVDALSECNLNLN"
     gene            29331..29900
                     /locus_tag="POB34_001462"
     CDS             29331..29900
                     /locus_tag="POB34_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein CI"
                     /protein_id="HDI5918441.1"
                     /translation="MNLEKGGRGAIERMVEAYGFKTRQALCDHLGISKSTLATRYMRD
                     SFPAEWVIQCALETGTSLNWLTTGHGSKQTSGNTNTMEVAKYVLSDGALREDGFYIFD
                     KGFLPSTFKKPFVITDNNSEFICDKEFDDIRDGKWVISIDGEITIRDITRLPGGRIFV
                     EGGNRAFECKIEDVEIIGKIISLTIKYVR"
     gene            29916..30107
                     /locus_tag="POB34_001463"
     CDS             29916..30107
                     /locus_tag="POB34_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918442.1"
                     /translation="MLGKVFFVVLSCSLLLNPLTTYAKNYPCSGKKGGVSHCTSDGKF
                     VCNDGTISKSKKICTKNSR"
     gene            30294..31346
                     /locus_tag="POB34_001464"
     CDS             30294..31346
                     /locus_tag="POB34_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI5918443.1"
                     /translation="MAVRKLTTGKWLCECYPAGRSGRRVRKQFATKGEALAFERHTME
                     ETEAKPWLGESVDRRTLKDVVELWFKLHGKSLTAGQHVYDKLLLMVDALGNPLATDLT
                     SKMFAHYRDKRLTGEIYFSEKWKKGASPVTINLEQSYLSSVFSELSRLGEWSYPNPLE
                     NMRKFTIAEKEMAWLTHEQIVELLADCKRQDPILALVVKICLSTGARWREAINLTRSQ
                     VTKYRITFVRTKGKKNRSIPISKELYEEIMALDGFNFFTDCYFQFLSVMEKTSIVLPR
                     GQLTHVLRHTFAAHFMMSGGNILALQKILGHHDIKMTMRYAHLAPDHLETALRFNPLA
                     TLPSGDKVAAAVGITP"
     gene            complement(31451..31987)
                     /gene="rcdA"
                     /locus_tag="POB34_001465"
     CDS             complement(31451..31987)
                     /gene="rcdA"
                     /locus_tag="POB34_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HDI5918444.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            32071..33279
                     /locus_tag="POB34_001466"
     CDS             32071..33279
                     /locus_tag="POB34_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5918445.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHISLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            33279..34094
                     /gene="ybjI"
                     /locus_tag="POB34_001467"
     CDS             33279..34094
                     /gene="ybjI"
                     /locus_tag="POB34_001467"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HDI5918446.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            34180..34464
                     /locus_tag="POB34_001468"
     CDS             34180..34464
                     /locus_tag="POB34_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918447.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(34505..35737)
                     /gene="mdfA"
                     /locus_tag="POB34_001469"
     CDS             complement(34505..35737)
                     /gene="mdfA"
                     /locus_tag="POB34_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HDI5918448.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGQFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            36022..36618
                     /gene="ybjG"
                     /locus_tag="POB34_001470"
     CDS             36022..36618
                     /gene="ybjG"
                     /locus_tag="POB34_001470"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDI5918449.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGFTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
BASE COUNT         9226 a         9482 c         8744 g         9236 t
ORIGIN      
        1 caagccagga agcctgcgat aagctacctt ccgggcgtgt gtacaacatc accaacggcg
       61 agcatcgcac actgcgcagc atcgtgcaga agctgatcga cgagttgaat attgactgtc
      121 gtattcgttc cgtcccctac ccgatgctgg atatgatcgc ccgcagcatg gagcgtttag
      181 gccgcaagtc agcaaaagag ccgccgctga cccactacgg cgtctccaag ttgaattttg
      241 actttacgct ggatattacg cgtgcgcagg aagagttagg ttatcagccg gtcatcaccc
      301 tggatgaagg tatcgagaaa actgccgcct ggctacgcga ccacggaaaa ctgccgcgct
      361 aatcctgccc gtatttctcc agcaatgctt cggcaatcgc ctgagtttct gcatccgctt
      421 cgccgttata taacgtcggg cggaaatgca tctggaatgc cataatcacg cgccgctgct
      481 cgcgcggtgt catatcaggt ttaacgtcat aaccgtagcg cgccaaaagc tccagcaatg
      541 acgcggtatc taccggtgtg tgcggcgcgc gcccggcaag gtaaaagtta acccgctgcg
      601 catccggcca ggcaccaatc ccctgctgcg ccagttgctg ccagggaaat aatggccccg
      661 gatcgtcttt gcgctgcggt gcgatatccg catgtgccac tacgttttcc ggcttgatgt
      721 gataacgggc aataatatct ttcgccagtg gaataagcgc ctgaatctgt gccggttcaa
      781 acggggcaaa atatttcact ccggccgatt tttgccagcc acggttttcc agctcaatgc
      841 caatagaggt gtcgttaagg cgcgttgccc cgcgccaggc gctaatcccc gcatgccagg
      901 ccagttcttg ttccggcacc agttgccaga tgcgcggttt accgttgtat cgcggtggta
      961 ccgcagggac cagataatgc gagctgacct gcttatcggt cagtgtcgcc agcgagctat
     1021 caaaatcatc tgcggtgtag tggatcacca gcactttaat gcgcggatac gccgcctgcg
     1081 cctggcgtcg ggtatcaagc tgatatccct ctttctcgac aatgcctttt tcgcctgcac
     1141 accctgccaa taacagagcg gcagcgacca gccagaagac tcttctcatc gacgcgtttt
     1201 caccgccgta ccgctgacgc taaccatcag catactgccg ttttgcccga ccgtttcgta
     1261 gtcgatatca ataccgacga cggcatcggc ccccagcgcc cgcgcctggg agcctaattc
     1321 ctcaaaggcg atctcccgtg ctttacgcag ttctttttca tacgcaccgg aacgtccgcc
     1381 aacgatatcg cggataccgg caaagaaatc acggaaaata ttggcaccta aaatcgcttc
     1441 gccggtcacc acaccgcaat attcaacgat ggtctggcct tccagagttg gggttgtaga
     1501 aaattgcatg atcgatctcc ttgtttgcta agccttcgat ctcaaaagca ttatcagact
     1561 gatacgctat tattgaaagg atatcattat tatcgtagaa cgctttcaga gcgatcgctt
     1621 ataaggaaat cattatgcgc tacagcaaat tgacaatgct tatcccctgc gcactgctcc
     1681 tcagtgcctg caccacagtc actccagctt ataaagataa cggcacacgc agtggtcctt
     1741 gcgttgaagg aggcccggat aacgtggccc agcaatttta tgactaccgc attttgcacc
     1801 gcagcaacga catcaccgcc ttgcgccctt atctgagcga caaactggcg acactgctta
     1861 gcgatgcctc ccgggataat aaccatcggg aactactgac caacgatcca ttctccagtc
     1921 gcacaacctt gccagagagc gcccgtgttg ccagtgcctc aacaatcccg aatcgtgatg
     1981 cgcgaaatat tcctctgcgt gtggatctaa aaaagggtga tcagagctgg caggatgaag
     2041 tgttaatgat tcaggaaggc cagtgttggg tgatcgacga tgtacgctat ttaggcggca
     2101 gcgttcatgc cacggcgggt acgctccgtc agtccatcga gaatcgctaa cgccttgatc
     2161 aaccaataaa ccccgtaaat tgtccggcat ttcgtgagga atgccgacat ttatgctcgc
     2221 cgaccaccgc ccccgttatt ttgtgctatg tttattgaat aatgcgcttt gcttttaact
     2281 tttaaagcag aaatattgca taattattct gtcaaaggta ctatcttcga cctcaatagc
     2341 tatcagactg ccagtatacg agtgtcaatg agtattcaat taaacggcat taattgcttc
     2401 tacggcgcgc atcaggcgct gttcgatatc acgctggatt gcccacaggg cgaaacgctg
     2461 gtgttacttg gccccagcgg cgcgggtaaa agctcgctgc tgcgtgtact caatctgctt
     2521 gagatgccgc gctccggtac gctcaacatt gcaggcaacc atttcgattt caccaaaaca
     2581 ccctctgaca aagcgattcg cgatttgcgt cgtaacgttg gcatggtgtt tcagcaatac
     2641 aacctgtggc cgcatctgac cgtgcagcaa aacctgattg aagcgccctg ccgtgtactg
     2701 gggttgagta aagatcaggc gctggcccgt gcagaaaaac tgctggaacg tctgcgtctc
     2761 aaaccttata gcgatcgtta cccgctgcat ctttctggtg gtcagcagca gcgtgttgct
     2821 attgcccgtg cgttgatgat ggaaccgcag gtactgctgt tcgatgaacc gaccgccgca
     2881 ctggacccgg aaattacggc acaaatcgtc agcatcattc gtgagctggc agaaacgaat
     2941 attacccagg tgatcgtcac ccacgaagtt gaagtggcgc gtaaaaccgc cagccgagtg
     3001 gtgtatatgg aaaatggtca tatcgtagaa caaggcgacg cgagctgctt taccgagccg
     3061 caaaccgaag catttaaaaa ctatctctct cactaagatt cgggataaca acaatgaaaa
     3121 aagttctgat tgccgcgtta attgcaggtt ttagtctttc cgccacagct gccgaaacca
     3181 ttcgttttgc taccgaagcc tcctatccgc cgtttgaatc gattgatgca aacaaccaga
     3241 tcgttggttt tgacgtcgac ctggcacagg cgctgtgtaa agagattgat gcaacctgta
     3301 ctttctctaa ccaggcgttt gacagcctga tcccaagcct gaaattccgt cgcgtagaag
     3361 cagtgatggc gggcatggat atcactccgg agcgtgaaaa gcaggtactg tttaccaccc
     3421 cgtactatga caactctgcc ctgtttgtgg gtcagcaagg caaatacacc agtgttgatc
     3481 agctgaaagg caaaaaagtc ggcgtacaga acgggacgac acaccagaaa ttcattatgg
     3541 ataagcaccc ggaaatcact accgttccgt atgacagcta ccagaacgca aaactggatc
     3601 tgcaaaacgg gcgtatcgac ggcgtcttcg gtgacaccgc agtggtcact gagtggctga
     3661 aagataaccc gaaactggcg gcggtgggcg acaaagtgac cgataaagat tacttcggca
     3721 ctggcctcgg catcgcggta cgtcagggca acactgagct gcagcagaaa ctcaacactg
     3781 cgctggaaaa agtgaagaaa gatggcactt acgaaaccat ctacaacaaa tggttccaga
     3841 agtaattcct gatgaatgaa ttttttcctt tagcaagcgc cgccgggatg accgtcggcc
     3901 ttgccgtttg tgcattgatt gtcgggctgg cgctggcgat gttctttgcg gtatgggagt
     3961 cggcaaaatg gcgtcctgtc gcgtgggcag gttcagcgct ggtaaccatt ctgcgtggcc
     4021 tgccagaaat tctggtggtg ctgtttatct attttggctc ctcgcagctg ctgctgacgc
     4081 tttcggatgg cttcactatc aatcttgggt tcgtgcagat cccagtgcag atggacattg
     4141 agaacttcga cgttagcccg ttcctttgtg gtgtcatcgc tctgtcactg ctgtatgccg
     4201 cctatgcctc gcaaacgctt cggggcgcgt tgaaagcggt gccggtgggt cagtgggagt
     4261 ctggtcaggc gctggggctg tcgaaatcgg ctatcttttt ccgtctggtg atgccgcaga
     4321 tgtggcgtca tgcgctgcct ggcctcggta accagtggct ggtgctgctg aaagataccg
     4381 cgctggtcag tttgattagt gtgaatgatt taatgctgca aacaaaaagc atcgctactc
     4441 gtacccagga accatttacc tggtacattg tggcggcggc gatttacctg gtgatcaccc
     4501 tgctcagtca gtacattctc aaacgcattg acctgcgcgc gacacgtttt gagcggaggc
     4561 ccagctaatg tttgagtatt tacccgaact gatgaaaggg ctacacacca gcctgacgct
     4621 aaccgttgcc tcgctgattg tggcactgat tctggcattg atttttacca tcatcctgac
     4681 gctgaaaacg ccggtgctgg tgtggctggt gcggggttat atcacgctgt ttaccggtac
     4741 gccgctgctg gtgcagatct tcctgattta ttacgggccg ggccagtttc cgactttgca
     4801 ggagtatccg gcactgtggc atttgttgtc agaaccgtgg ttatgtgcgc tgattgcgtt
     4861 atcgctgaac agtgcggcgt ataccacgca gctgttttac ggtgcaattc gtgcgatccc
     4921 agaaggtcag tggcagtcct gtagcgccct gggaatgagc aaaaaagata cgctggcgat
     4981 cctgctgccg tatgccttta aacgctcgct ctcttcttat tccaacgaag tggtgctggt
     5041 gttcaaaagt acctctctgg cttacaccat tacgctgatg gaagtgatgg gatacagcca
     5101 gttgttgtac ggacgcacct acgatgtaat ggtgttcggt gcggcaggga ttatttacct
     5161 ggtcgtcaac ggcctgctga cgctgatgat gcgtctgatc gagcgcaaag cactggcatt
     5221 cgaacggcga aattaatgta gctgcgtaca ttcgccgggg gggtttgtag gtctgataag
     5281 acgcgccagc gtcgcatcag acatcagcac ggtgcctgtg ccggatgcgg cgtggacgcc
     5341 ttatccggcc tacatagtgc ataaattctt ataatgaaga cggacaactc actaaggtat
     5401 ccgtcttttt ttatttcatt taaattattt aatcatattt attgcatata aattcacttg
     5461 atggcattgt tatcccatgc cgcagacacg gccaaaaatc ataagattga cagacgggag
     5521 ttccatcatg aaaaagttag ttcttgccgc tttacttgct tcctttactt tcggtgcttc
     5581 tgccgcagag aaaatcaatt ttggcgtttc agccacctat ccaccctttg aatctatagg
     5641 tgctaataat gagattgtcg gctttgatat cgatctggca aaagccttgt gcaaacaaat
     5701 gcaggcagaa tgtactttta ctaatcacgc gttcgacagc ctgatcccgt cactgaaatt
     5761 cagaaaatat gacgccgtaa tctccggtat ggatatcacg cccgagcgta gcaagcaggt
     5821 atcgtttacc acgccctact atgaaaactc agccgtcgtg attgccaaaa aagataccta
     5881 caaaacgttt gccgatctga aaggcaaacg tattgggatg gaaaacggca ccacgcacca
     5941 gaaatatatt caggatcagc accccgaagt gaaaactgtc tcctatgaca gttatcagaa
     6001 tgcctttatc gatctgaaaa atggtcgtat tgatggggta tttggtgaca cagcggtggt
     6061 aaacgaatgg ctgaaaacca atccgcaact gggcgttgct actgaaaaag tgaccgatcc
     6121 gcaatacttc ggcaccggtc tgggcatcgc tgtacgtccg gataacaaag ctctgctgga
     6181 aaaactgaat aacgcgctgg cagcaattaa agctgacggt acatatcaga aaatcaatga
     6241 ccagtggttt ccgcagtaat tgagactgat gacaaaagta agattgcctg atgcgctgcg
     6301 cttatccggc ccacatcaac tctgcaatgt attgatttag catgattttg taggccggat
     6361 aaggcgtaca cgccgcatcc ggcatgaaca aagcgcacct gatcagcaat ctgaagccgc
     6421 acctgcggct tttttattgc ttcaccagca gcgtcagcac ttcatagtgc gcggtgtgcg
     6481 ggaacatatc aaaaagctgt acccgttcga tgcggtaacc tggcagttcg cggatatctt
     6541 tcgccatggt ttgggcgtta cagctggagt agataataaa acgcggtgcc attgttgaga
     6601 gataatcaca tagcggttta ccaatgccgc ggcgcggcgg attaaccagc accagctccg
     6661 gcacctcccc ctgagcggtg gcaaactgag tggagtccag cgcctgaaat tgcaaacgcg
     6721 ttaagcccag ttcagcggct gactgctttg cacaggcaat ggcctctgat gcaatttcga
     6781 tcccggttaa ctgcatgtca ggcgtcgcgc agtgtaaacc aaagccaccc acaccgcaga
     6841 acagatccca catatgttta actggcagct gccgtaccca gtcgcgcgcg gtagcgtaca
     6901 gctggctggc gaccgccgga ttagtctgga agaaactttg cggacggatc cacagcggta
     6961 cgtcattaaa acgctctgcc agcgcctgtt gttcagtaag gtagatctcc gtctcccctt
     7021 ccataatcgc catatgtacc ggctgaatat tgacggtaat aactttcagt tgcggtagtt
     7081 gttcctgtaa ccacggcagc gccttacgca gttgtgccag tttggtttca gaacgcagta
     7141 caaagcgcag catcatgccg ccatcgctct ggctttcagt cagcagaatg tatttcagtt
     7201 cgccacgctt acgcgccacg ttgtagggtg tcagccccgc acgggcgata aacggtttta
     7261 gcgccgcaaa aacgggcgca aatgaggcag gataaagcgg gcagtcacaa aggtcttctg
     7321 gtgtgccgtc gcgatgcagc ataccgagca gtggtttttc aacgctacca ctcaccacca
     7381 ttttggcttt attacgaaac ccttgttccg ggcctgacac cggcgcgcac cattcctcaa
     7441 ccggaaaatc ggcgagcaga tttttaagat cggcggtttt agcggagagt tgctctggaa
     7501 tcggctgcgt tatccactga caggaacgac agcgacccgc gtcgtaaagt gcgcactgca
     7561 tacattgacc ttcacatcat caggggcgac gattatacac attattgcaa ctggaagaac
     7621 cgccgactgg tcgagggaac gaacagcagc atcaggatga gcatatccgg cagcttctgc
     7681 agcatcaggc tatggaagat ttcacgtttt gattcaccgg gaatgctgaa cagctccgga
     7741 taaccgtacc ccagcgaagc cgcccacaag taacttgcgg cggtgatttg tgtcagcaga
     7801 tatagccagc gcgcccagcg acgccctttc accagtgaaa acgcacacca gatctcaatg
     7861 aaaaccagca ccagactgct taaaaagacc agcgttaaac tccaggtttg tacgctgcga
     7921 tgaatgaact cgccaatacc gcgcacgccc agcgtattga aaatcatcag cacgtcgagg
     7981 ccacggatca taataatggc gagcgccgct acctgcacca gcgcaggcac attcaggcga
     8041 gcatgagatg aagatgtttt cttaaaaaat cccaacgttt cgtcttccat gaaaataatg
     8101 ccgcggcatg cgcggcatta tgtagccagg ttggcaaatt ttagtgtctt cagccatgtc
     8161 ttgcacgctg gatatcgcgt atccgctgtt tttctgcgcg agccatcaga taccaggcga
     8221 taaatccgac aattccgacc gcaccgagaa ttaacgttgc aagggcgttg atttccggat
     8281 tcacccccat ccgcacgctg gaaaagacca gcatcggtaa cgtggtggct cccggcccgg
     8341 aaacaaagct ggcgatcacc agatcatcaa gcgacaaagt aaaagccagt aaccagccag
     8401 aaatgatcgc gggcatgatc atcggtagcg taatgacgaa aaacactttc agcggcgtcg
     8461 caccgagatc catcgcggct tcttctatcg agcgatccag ttcccgcaat cttgaagaaa
     8521 taacgaccgc cacataagcc gtacaaaaag tgacatgcgc cagccagatg gtgagcatac
     8581 cgcggtccgc aggccagcca atggcatgag caagcgcgac gaataataac aacagcgaca
     8641 agcccatgat gacatctggc attaccagcg gcgcggtgat cataaaggca aagccatttg
     8701 atccgcgaaa cctgccaaag cgcaccagca ccaccgccgc aatagtcccg aggatcgccg
     8761 ccgccgttgc cgcacaggca gcaattgtca ggcttaaacc aaccgcactc atcatcgcgt
     8821 catcgcgcaa taactcacca taccagcgcg ttgaccagcc ggcccacacc gtcaccagct
     8881 tcgagctgtt aaacgaatag ataaccagca tcagcattgg cgcgtagaga aaggtaaagc
     8941 ccagcagcaa aatcacaatc cgccagggtg aacgaactac cggtaaatta ttcatccgtg
     9001 ttctcccacg cttttttgct ggtgtttgtg gaaccacatt atcggcacaa ttagcagcag
     9061 caacatgatg atcgctaccg ccgaggccac cggccagtcg cggttattga aaaactcttg
     9121 ccatagcacg cgcccgatca tgatgctgtc cgggccaccg agcagttccg ggatcacaaa
     9181 ctcgcccaca gccgggataa acaccagcat cgatccggca ataatcccac ctttagtcag
     9241 cggcacgatc acagtaaaga acgttttcag cggtcgtgca ccgagatcca gcgctgcttc
     9301 caccagcgaa taatcaatac gaatcaacgc ggtataaatc ggcagtacca taaacggcac
     9361 gtaggcgtaa acaatgccaa tataaactgc cagattggta tgaagaatgg tcagcggttg
     9421 atcgataacc cccagccaca gcagaaaatt attcagcaca ccgttgtttt ttaatattcc
     9481 catccaggca taaacgcgga tcagaaacga ggtccacgac ggcaggatca ccagtagtaa
     9541 taaaatatta cgggtcgaag gcttactgtg cgccaccgcc cacgccagcg gatagccgat
     9601 cagtaaacag caaaatgtcg aaatcgcagc cacctgtagc gactggagat aagcatcgaa
     9661 atagagcgga tcgtcggtca gttgcagaaa attaccgaga ttaagagtga tggacagttg
     9721 cccgtcagcc cactccatca gttcggtata aggtggaata gcgcgcgcca tctccgacag
     9781 gcttatttta aagacaatca gaaatggcag cagaaacagc aagatcaacc agatatatgg
     9841 caacgcaatg accagtttgc gcccatgctt catttgcagc tgcgacaacc acagcttaaa
     9901 tccgcccggc ggtttcgact gggcagcagg ttcaagtgta ttcatctttg ctccttaaac
     9961 cgtcagcacc acacagctgt ccacttccca gcacaaacgc acttcgtcgc cccaggtcgg
    10021 taacccttta cgatgacgat gggcgttttg tagctgggcg ctaatcatct gcccactttt
    10081 cagacgaacg tgatacaccg aaagatcgcc gagataggca atgtgtatca cctcccccac
    10141 cgcgaagtta caaccattgg cgggcggctc ttcgcaaagc atgatttttt ccgggcgcag
    10201 cgctacgtgt accggcacgt tatcgaccac tgaagcatcc gcatcgactt tcagtggatg
    10261 caccagcccc ggcgaatcaa gcaccaggcc atcttcctga cgctctttga gtacaccttc
    10321 aaagacatta accgaaccga taaattcagc gctgtagcgg gtagttggat gctcgtagat
    10381 ctcttccggc tcgccaatct ggacaaattt cccgcgattc ataatggcga tacgccccgc
    10441 catggtcatc gcctcttcct gatcgtgggt gaccatcaca caagtcacac cgacgcgctc
    10501 cagaatatcc accacttcaa gctgcatccg gtcacgcagc tttttatcca gcgcgcccat
    10561 cggctcatcg agcagtaata gtttcgggcg tttggcaagg cttcgggcca gggccacacg
    10621 ctgtcgctga ccaccggaaa gctgatgcgg tttgcgtttg gcgaactcct gcatgtgcac
    10681 caggccgagc atctcatgga cccgactggc aatttccgct ttcggtagtt tgtcctgttt
    10741 caggccaaaa gcgatgttct gttccacggt catatgcgga aacagcgcgt aagactgaaa
    10801 catcatattg atggggcgca ggtaaggcgg cacctgtgac aaatcgacgc catcaagcat
    10861 tatctgtccg gcagaaggtt gttcgaaacc tgccagcata cgcagcagcg tggacttgcc
    10921 acagccggat gcgcccagca gcgcgaagat ttcacctttg tagatggtca ggctgacatc
    10981 atccaccgca tgttgaccat cgtaggattt ggtgaggttg cggatttcta atagcggcgt
    11041 cagcgcctta cgggttttcg cctgcgggcg agggatagcg tcattcacgg ctgctctccg
    11101 gcattgaatg tgtgcaagcg caccatgatg gtgcgaattg ccggcgggtg tggtgcgccc
    11161 gcccggcatc taaggctgcg gattattttc cgctcttcac tttggtccac gcgcgggtgc
    11221 gcacacggtc gattttcggg tcctgcactt tcagagtgaa cagcttcgca cgaacatccg
    11281 caggcggata aatacccggg ttctcacgga cttccgcact caccagcggc gtggctgctt
    11341 tattggcgtt ggcatagaac acatggtcgg aaatatgcgc caccacatcc gggcgcagca
    11401 ggtaattcag gaactgatag gcttcgtctt tatttttggc atcggcaggc atggcgaata
    11461 catcaaagaa tgccatcgcc ccctctttcg gaatcgagaa cgagacattc acgccattct
    11521 tcgcttcctt cgcgcggttt gacgcctgcc agacatcgcc tgcccaacca atcgctacgc
    11581 agatgtcacc attcgccagg tcattaatat attgcgatga atggaaataa cgaatgttcg
    11641 ggcgcagctt taacagcaga tcggtagccg gtccggtgta atcatctgct ttagtgctgt
    11701 tgggatcttt gccgagataa ttcaacacgg tagcaaaaac ttcttctggc gcatccagga
    11761 aagagacacc gcagcttttc agtttttcca gattttcagg tttgaggatc aagtcccagc
    11821 tatcgacggg cgcgttttcg cccagcacag ctttaacttt atcaacgtta tagccaatcc
    11881 cggtcgtcgc ccacatatag ggcatagcaa atttattgtc gggatcgtgt ttggcgacca
    11941 gcttcagcag ctccgggtcg agattcttcc actccggcaa tttgcttttg tccagcggct
    12001 ggaaaactcc cgcagtcaac tggcgttcaa gaaagctggc agatggaacc accaagtcaa
    12061 agccggtgct acccgccatt aatttgcctt ccagtacttc gttagagtcg aaaacatcgt
    12121 agacgacttt aataccggtt tctttttcaa aattggccac cgtgtccggg gcgatataat
    12181 cagaccagtt ataaacgtgg agtgtttttt gttcagccgc gagcgtgccg acagagacgg
    12241 ccatcagagc acccgcagcc agacccgaaa gccatttttt atttaaggcg gtcatatctc
    12301 tttccttctg aaagttcgtt aacaaacgaa caaaagttgt cacactaaaa gatatgcaat
    12361 aaacgtgcat attttttatc ggcggggtgt aagaaaagag aactctctcc cggccagcac
    12421 tgctcgctat ataaaagcat cgcaagaata actttagcca gggtttgaga ctatagctcg
    12481 gattaaaaaa cggtatttaa caaattttta tgcgtatttg ctctatgtga taaagccaaa
    12541 tcgacacaag agaagtgaaa taatctttaa gaaaaggtta aaaacagcag ataattttgc
    12601 agcgcgcagc cactgcacca gcgactgcgt gctcgaaagg agttatcagt ggaggaaata
    12661 gttttcggta acgttgtttt gcccttcatc gtcagtcggt aacagcagtt gatgggcatt
    12721 agcttcaaga atgaccatcg aaatctgctc ttcgctctga cgcacgaacc atgcaaactg
    12781 ctcataggtt acgccctgca taacggataa agactggcaa accaccagct ttggcagatt
    12841 atcatcctgc atatcaagaa atgctttcac ggtcagcgaa ctggcattga tggcagataa
    12901 atccgccgcc agcggcaata cggctgaagg tcggacttcc gccatggcag aaaacaggat
    12961 cgtgttatcg atcagatcga ttttggcatc aaagacaccg tcgaaattct gcatatgggg
    13021 cagatgcaga gcctgacagt tatcacattc aaaaaaactc atccccaggt catcgagcca
    13081 ttgacgcagc gtatccagac caggaacgac cagcgatgtc ataattttgt acaacctctt
    13141 tcgataaaaa gaccggcaca gcttacgcaa aaagcgcagg caaaaccatg atcagtaatg
    13201 tgattgcgac taaccacccg ttttcaggca atattcggtc gtagcgtggc gttcgatcca
    13261 gcggatcatt ttacccgcga tgtcgatacc ggtggttttt tctattcctt ccagccccgg
    13321 cgacgcattc acctccatca ccaacggccc gcgattagca cgcagaatat ccacaccagc
    13381 aacgtccagc gccatcgttc gcgcggcttt tatcgcgatt tcacgctcct gtggtgtgat
    13441 actggcgacg cttgccgcgc cgccacgatg caaattggaa cgaaaatcgc cctctttcgc
    13501 ccgccgttca atcgcagcga ccacttcatc gccaacaacc agacagcgga tatcgcaccc
    13561 ttgcgcctct ttgatatatt cctgcaccag aatatgcgcg ttcagaccgc ggaaagcgtc
    13621 tatcacgctt tccgccgcct gacgcgtctc cgccagcacg acgccaattc cctgcgtgcc
    13681 ttcaaccaac ttgaccacca gcggcgcacc accgaccatg tcgattaaat cgctggtatc
    13741 atccggcgaa tgcgcaatgc ccgtgacagg caggtcgatg ccctgacgcg ccagcagttg
    13801 catggaacgc aatttgtcac gcgcccgggc aatggcgacc gactcattga gcggatagct
    13861 ccccagcatc tcgaactggc gcagtgccgc cgtcccataa aaggtaatgg cggtgccaat
    13921 acgcgggatc actgcgtcaa aatggggtaa cttgcggcct ttgtagtgaa tagaagacgc
    13981 cgcaggattt atgttcatgt agcaagaaag cggatcaaga atttcaacca ggtgaccgcg
    14041 ctgtatagcg gcttcacgca gccgcttaca cgaatagagc gttccatccc gggacaatat
    14101 ggcaattttc accctgcacc tctctgtcag acctggtcaa aagcccgcgt atcatacatc
    14161 gacgtggcgg ttttagcgcg tcgcccaacc ctgtttgtgc aaataatcca gaataaatgg
    14221 gcggctttct ttaatgattg ttcggcggat atgatcgctc caggtatccc ggcgattatt
    14281 gctgccacgg gtgaggtaat attccgccag ttgctcgtca tactgcgcca gtgcgccttt
    14341 atccagcggt tgatagctgt tttcatgcac caaaatggag gccggtaaac gcggcttaag
    14401 atccggatta tccgcaggcc agccaaggca cagcccaaac agcggcagaa catgctgcgg
    14461 taatttaagc agtttcgtca ccgcttcaat attattgcgc aggccgccga tatatacccc
    14521 gcccaatccc agcgattccg ctgcgattaa tgcattctgc gccatcattg ccgtatcaac
    14581 gacaccgagc aacagttgtt ccgccaggcc gagctgagca tccggacaga tctgtaaatg
    14641 gcggttaaag tcggcacaga acacccagaa ctccgccgct tgcgctacgt gtttttgccc
    14701 gccggtcagc gtcaccagtt cttcacgtaa cgctttgtcg gtaatgcgaa taatgctact
    14761 gcactgcaaa aaactggaac tggacgtcgc acgggcgctg ttaataatcg cctcacgctg
    14821 cgcttcggaa atgggttcat cagtgaaatg gcgaatggag cgatggccac aaataagttc
    14881 aatggttggc gtcattatct ttttctcttt ctgaacgtga atattgcggt ggacggttca
    14941 tcagctgtgg ggcaagacgt tttgccacct gaagaataac caccaccgca gcgggaagca
    15001 tgagcaaaac accgagaaaa atcatcagaa tctgcacttc tggccgagaa aatggctcag
    15061 gcagcgacag ggagtcgctt accgacagca gcgccaccgc cagtagcatc attccgataa
    15121 attccagtat caacacgcct ttaggcaatt taccgatcgc gcgcatacgc ttccctctgc
    15181 aaagtgagcc ttcagtctaa aacttttcac tgtattgtgt ttaacagtta tagcttttag
    15241 caattaatgc aacaggttaa acctactttc agcgaataca ttttagcgtg atcattacag
    15301 gcataaatct atgaggagag aaataatgca aaccgttatt tttggtcgtt cgggttgccc
    15361 ttactgtgtg cgtgcaaaag atctggctga gaaattgagc aatgaacgcg atgattttca
    15421 gtatcagtat gtagatattc gtgcggaagg gatcactaaa gaagatctac aacaaaaggc
    15481 aggtaaaccc gtagaaaccg tgccgcagat ttttgtcgat cagcaacata tcggcggcta
    15541 taccgatttt gctgcatggg tgaaagaaaa tctggacgcc tgatcgtctg acaagccctc
    15601 gcgttgaggg ctttactgat tttttctgtg ctgtggttta aacaaactac tgataaataa
    15661 gaaacacagt gcccccagcg cacaccagaa caccgcgctt agtaaccatg ccagctcttg
    15721 ccagaatgag cgcgtcggtg aaaaaaacag ccgcataatg agcatcgaac agggtgccgc
    15781 cagcattgcg ccaaacagag gtttcaggac ttctctacgc tgtgaaaaga agctggcgac
    15841 tgctccagga agaatgaaaa atagcaagcc gatttcagga tgcccggcag cccgaaaagc
    15901 gcctttcatg tgcgtcgcca gaaaaaggca caccacaatg aagaggacaa aacagcagat
    15961 tgcccccgcc caacgttgtt tatgtttcac tcgttcctcc tgacactgcg tctatcgaac
    16021 acatttttcg ccagtgtggc gttcagtaag ataaagccgc ttcgcattcc atgctaatat
    16081 aggccaacgc aattcatata gccgttgata cctaatgtga ttacactagt aaaatatatt
    16141 gttactttac tatcgtttag gtgcgctgaa tgaatctgcg ccctgaattc tggtaaaaaa
    16201 cattatcgta aattaccatt tctttcaaca gcttactagt aaacaagaag ttagcctccg
    16261 tgaatataaa cgtcgccgaa ttgttaaatg ggaattacat tctgttatta tttgtggtcc
    16321 tcgcgcttgg gctatgtctc ggaaagttac gacttggttc gatccaactg ggtaattcca
    16381 ttggcgtttt agtcgtatcg ctgttattag gccaacaaca tttcagcatt aacaccgatg
    16441 cgcttaatct tggctttatg ctgtttattt tctgcgtcgg ggtcgaagcc ggaccgaact
    16501 ttttttccat tttttttcgc gatgggaaaa attacctaat gttagcactg gtgatggttg
    16561 gcagtgcgct ggtgatcgcc ttagggttag gtaagctgtt tggctgggat attggcctga
    16621 cggccggtat gttagcaggc tctatgacgt cgacaccggt tctggtcggt gctggcgata
    16681 cactgcgtca ttccggcatg gaaagcaggc agctctcact ggcactggat aatctgagcc
    16741 tcgggtatgc cttaacctat ttaatcggtc tggtgagttt gattgttggt gcgcgttact
    16801 tgccgaaatt gcagcatcag gacttacaga ccagcgccca gcaaatcgcc cgcgaacgtg
    16861 gcctggacac tgatgccaac cgtaaggttt atttaccggt gatccgcgcc tatcgcgtcg
    16921 gcccggaact ggtggcctgg accgacggca aaaatctgcg tgaactgggt atttatcgac
    16981 aaaccggctg ctacattgaa cgtattcgac gtaacgggat tctggcaaat ccagacggtg
    17041 atgccgtgct acaaatgggc gatgaaatag cgttggtagg ctatcccgac gcccatgccc
    17101 gactcgatcc cagcttccgt aacggtaaag aagttttcga tcgtgacctt ctcgacatgc
    17161 gtatcgtcac tgaagaagtg gtcgttaaaa accataacgc tgtaggtaaa cgtctcgcac
    17221 aactgaagtt gaccgatcac ggttgcttcc ttaaccgcgt cattcgtagc cagattgaga
    17281 tgccgataga tgacaacgtc gtgcttaaca aaggtgacgt tttacaagtc agcggcgatg
    17341 cccgccgcgt aaaaaccatc gccgatcgca tcggctttat ctcgattcac agccaggtca
    17401 ctgacctgct ggcattctgc gccttctttg ttattgggct gatgatcggg atgatcacct
    17461 tccagttcag cacattcagt ttcggcatgg ggaacgctgc cgggttgtta ttcgccggaa
    17521 ttatgctggg ctttatgcgt gctaaccacc cgaccttcgg ttacattccg caaggtgcat
    17581 taagcatggt gaaagagttc ggcttgatgg tgtttatggc aggcgttggt ctgagcgccg
    17641 gtagcggtat taataacggc ctgggcgcga ttggcggtca gatgttgatt gccggattaa
    17701 ttgtcagtct tgtgcccgtg gttatctgtt tcttgttcgg tgcttatgta ttgcgaatga
    17761 accgcgcact gttgttcggc gcaatgatgg gcgcacgcac ctgcgcgccg gcaatggaga
    17821 tcatcagtga tacagctcgc agtaacatcc ctgcgctggg ctatgcgggc acctacgcaa
    17881 tcgccaacgt cctgctgacg ctggcaggga caatcatcgt catggtatgg ccaggattag
    17941 gataaaactg aagttgccct gaaaatgaaa tttttttgca caaccgcaga acttttccgc
    18001 agggcatcag tcttaattag tgccactgct tttctttgat gtccccattt tgtggagccc
    18061 atcaaccccg ccatttcggt tcaaggttga tgggtttttt gttgcctgaa atttatgcct
    18121 tttaaaatca tgatgttaga agcactgttt tttaacgatg gcgacaaaat ggcggcagcg
    18181 tcaaagagag agcgccacct gtcctgattt cattggatgc ggctgaaccg gatttgactc
    18241 ttttggcgtt gcaatcgaac gaacaaaagt ttcatgggta acaaaagtat ggctgcagtt
    18301 aatgttctgg cactggttgt aacgctcttt ggtcaatgaa gatacctgaa aactgctgcg
    18361 agtatgggcg gcacttccac acagtgggca aatcatcatt tttcgtgttc tccccatttt
    18421 tgctaaattc acaataatga taccgcatta ttccattttg caaacttaaa agttctccat
    18481 tgcgaagaat cattccattt cgaaatcatc aatcctcact tcaagctcca gactggtcgt
    18541 aaaaccgtta tccgggctga cggtatgcgt cagagtcgta atggtccatt ccgcatcatc
    18601 tatcggctgt ttaaagccac tgactttcac tggcatttcc gtgtagagat ctgcccgccc
    18661 ttccgccagt tgtagcgaga atgatgcaac gccgcgttgc aggcgttccc actgcatttt
    18721 cgctgcccgt tcggcgttgc tccggttggc ataagtgcga ttaagtacca gcacgttttc
    18781 atccgtaccc accaggtaat cgccctgctt cgcttccggc tctttcttct gcttcttagt
    18841 cctgcgctta cgcttcaccg tggtgctttc tttcttcgcg ggttcgcggg tatgcaacca
    18901 actggcaatt acgcccgtgt aggctccgcg atctgccagg gtaaagcggt gactgtcgcc
    18961 gtccttacgt gtgattgaga tcaccggcag tggtttaccg ctggcgcttt tgccctgtcc
    19021 ctgccggatg aataacagat tgccattttt caccgacgcg atggcaccgt actggcgcgc
    19081 cagccgcatc agaaaactgc cgtcactctc attagtctgg tctatatgct ccacgggctt
    19141 atccgacagg tctttaccca gtgccatctt cagcttatga cgcgcggcta tttccttcac
    19201 cagttccccg acagtggtct tgtgccacga tttttcacgg cgggtattca gcgtttccct
    19261 aaaatcagca cttcgcgccc ggatagtcag gcgatccggt gcgccggtgt gttcaatctc
    19321 gtccaccgtg aatgcccctt tcgggaaaag cggctgcccc ttccagccca gcgccagcgt
    19381 gatgaccgca ccacggcgcg gcagcacgat ttttccgtcg gcgtcgtcca gctccagatc
    19441 aatctggtct gcttcaaagc cccgattgtc cgtcaacgtc agactcatca ggcggttatc
    19501 cagcacagtg gtgatatcct tcccctcaat actgatgctg aatgcgggag ttttgttgcc
    19561 tttgttaagc agttcagagc tgaaattcac gacagcagcc ctcccaccgt tttactgata
    19621 tcgcttaagg cagacgttgc cgtatcctgc aaattattca gctgcgcact gagatcaccg
    19681 aacatatcgg acagggattc atccacccgt ttgagcgaca gggtaaactc aatccggcgc
    19741 ggcataccat cgcggaaaaa ctccgtttta gtctgattca gtccctcaat cacatacatg
    19801 ccgtaaatcg tgccgctgcc ttcaatcaga ggccatgctt tcccctgttc tgccatctgc
    19861 tccagtgcca gcaacgacag cctgccgcct gttatctccg gcataagaac accggaaagc
    19921 gtcagcatgt cgttgtccgg tcccagaaac tgcgtggacg gacgacgatt tacccggctg
    19981 ttagccgcat gtcgccagct gcgctgatac tgcagttcct gatacggcac ggtgcgcagc
    20041 ataaacacgt acaatcccag caccatcatc atgcgtcgta tcccccctga tcgctgtagt
    20101 tactcctggc ttttgccttc agcctgcgtt cacgttcatc aagctggcgg gccacctccc
    20161 gcgcaatatc ctgcgcactt tgtcctggct gcgtctgaat aatgatctgc gtcggtgcct
    20221 caatccgttg agcgggcggc acagtggctg cacgactcac cattgcttca ccgcctttcg
    20281 tgggaagtgc caaaggatgc aacggtggaa gctctgctgg cgcggcagca acacccatca
    20341 ttccggcaac aacggcagcc agtgcagctg tatttctccg gctggtcaca tttgccgggc
    20401 cgttaacaat ttccggcccg ttttcaccga cgatgccaaa ctgcccgcgc gggatatagc
    20461 cgccgctgtc atacatcccc gcaaagccat atccccatgc ccggttatct ttctgccatc
    20521 ccatccgctt tgctcaacga gtccgccacg ctgttccgcc gcaagtatta catcaacggc
    20581 agccatgcag gcttcatcat gtatatgacc gatgccgcgc agaatcagga ggatgtgaac
    20641 aatctccgca atgcgatgaa aagcgccaaa ggccctggca acttccgcaa cctgtttatg
    20701 tactcgccta acggcaaaaa ggacgggatt cagatcatcc cgttgtcaga agtcgcggcg
    20761 aaggatgagt ttctgaatat caaaaatgtc agccgcgatg acatgatggc agcgcatcgt
    20821 gtgccgccgc aaatgatggg gattatgccg aataatgttg gggggtttgg ggatgtggag
    20881 aaggcaagta atgtatttgt tcgtaacgaa cttatcccct tacaaaaacg tatcgaacaa
    20941 ttaaatcaat ggataggaaa cacaattatt aattttttac catattctct ccaaagttaa
    21001 aaaatagcat atgggtgcat aatgtttatg caccctctta ttaccagtta aaaattgatg
    21061 agctgcgaat tgcaggaaca tccgatggat ttcgtttagc agctggttcc atcaacttac
    21121 gcttattaaa tgcctgatca atataatact caggattttt aataaagaca gagtatttta
    21181 ccaatgcaca atatacgtca gagtcattat aatggggaac tgttttttca taaacatgat
    21241 tctttctaac cagataaggc aaataataat catatcccaa acctggcgtt cttagactta
    21301 tagaacatcc gcatgatgga tctgcacata ctgcaggagt aaacacactg tcgtaaattt
    21361 catctctgat aacaccaatt agaccattat agttttctcc ccagagtgat gcttgaattt
    21421 cagtgtttac aaatggacga tttttggttt ccgttccaat aagaacgaca gtaaccgttg
    21481 agtcttttaa aaaatcctca cgaatagtcc tcattattgt ttcatcagtg ttagttgtac
    21541 taatgtcacc atcacttacc gacttatcaa taaacgattc tcctccgaat aaatcaataa
    21601 tctcatcttt gagatcttgt tcgttagcat gatgataact aagaaacgtc ttatgcatta
    21661 ttcactccat ttatcaacat ttatatcaaa ctctttgact accttatcat tgaagaaatc
    21721 gtttacttct ttaaaacctt ccaatacttg actaaaatga taataatgct tttgtataat
    21781 acttataggg gttaaatgat acggcaatga tataaattcg ccgttagggt ctctatcctt
    21841 tgtggtcact ttatcatgat ctgaaacata tttatgccat ctccgataat tattttgatg
    21901 ttttgttaaa aaagagttta aggttttcaa aatttcattt gaaagtaaat acagtttttt
    21961 atctcttttt ttcttcacat ctagtaattt catttcatct ctaatgaatt tatagcactc
    22021 ataatatgag tttaaaactt cttcaattga gtcataattc ggatcaaatt taaataatgt
    22081 tcgcttcgta tccagataat tttttacgct ctttttaaaa agaagatcag acttatttag
    22141 taaaatatta ataccaccaa aactaagttg ctccaatctc aatgaatatc tttgatgaaa
    22201 tgcaccgacc agcaagatat aagcaactaa aaatagtatt aatactgata aaaaagcacg
    22261 aattatgctt acatttgaca tgaaattaac tataaaccac catttttcaa acggtgaaaa
    22321 cggaatgctc cccatgataa taagtagaag aacaaagata caactccata aaacccacaa
    22381 tctgtacata agaaagatcc tttttctttt tataatactg caactacacc tcaagtcaaa
    22441 accctcttct gatattaccc tctactacag taacccatca catgacctcc gacttatcag
    22501 aagactagcc aaactttctc aatcaaatca ggttgcgcgc gctcgtatcc ccgccacgcc
    22561 tgcccgcttt atgtagtggt tttcatgcac ctgcatgatc tacgcaaaag cccgccagtt
    22621 ctggcgggcc ttagcaaaaa cgatcctcaa acgatcatgc gatctcatgc ggcatagaca
    22681 tgcactacag agctaacgcc tcgcaagggc tcgttgttca accttgctga cgccagaaac
    22741 aagttcagac gccagcaacg tttcttaatg cagccagctg tcgtcttccc acaccttctg
    22801 cataattttc atcactcgtt ttctttcttc gtccagttgc agtccggtca gttccacacc
    22861 gttagagcta cctttgcgga tacgaattac cgttttggga tacagggggc gcagattgcg
    22921 gtaaagctcg gattcaaggg cgtccagggt agactggcta atcttctgct ctttatcgat
    22981 cattatttca atgcgcataa aagtcacctc aattgatgac atccattgag cggttgtatt
    23041 cgtgggttct gatttttgcc atgagttcat ctgtcagttc agaaacccac tgcagggcca
    23101 gccccttctc ttcatcacta cactcactag ccgctacaag cttaagaaaa aaatcaatgc
    23161 gctggagctt caaagactcc aaaaaatagt cctgcatctt tcctcctatg acaccacaag
    23221 aaatactgta cgtataacca ctgtttatat ttacagcata taataatctt actgatgtaa
    23281 aacgtttttt tacgttcatc agcctgatat gcctggtatt attaagagca cgaattgtta
    23341 acccgcgtaa ttaatacagg ttccgccatt tatcatcttc cttcagacgc tggttccgat
    23401 agaagatacg caggcctgct cctgatggaa tactgccacc gcgaaggagc aaatcgactt
    23461 ctttctcgct gccatcaaat cctctggact tcagttcata gacgagctgc tgatgctgat
    23521 gatctgtaat tcgctgtttg tagtctttac accttttcgg tttaaccagg cgcaacctta
    23581 ctgccagctc ccggcgatct tttttgctca tactgtgcag gtaatcgtgc aactccttgt
    23641 catccatgcg ggtaatgtcc gttctggtgt ccccatcagc tgatttatct ttctcctgtt
    23701 ggttcaaatt ttcagcaagg ggacagttat tgccacgagt ccaaggggcg caagcgccct
    23761 ggtcggctgc cgcctcctga acatcaacgg ccttacgaac cattttccac ttcacggcat
    23821 gagtgcagat cttgccctct gcaataggtg accagatgcc ataaatacga atgccgtgat
    23881 cgccataggc ggtcggctct tcgttgattt cataagcggt tctgatgagg tgatatttgc
    23941 ggggaaccag tacgccgccc tgcttcatga tgtaggtggc aaaacaacca gcatcagcag
    24001 cagccaggat ggcatcaagg cgcgggttat ccagtacccg cgcacctgct tttttgtccc
    24061 cctgttgcct tgccgcctga ccagccagca atcgcagttc acggtaagcc tgacgccccg
    24121 gaatgccaaa gaagcggaat tgctgaacac gatgcagaga cgcccaggca ttaacgtatt
    24181 cagcattatc acgcagggat ttccccgttt ccttgctgat ctcgccagcc agaccacgcc
    24241 cgtcaatgtt cttactgatg tatttcgcga tgtagcttgt tggcgtacct ttgcgcgggt
    24301 taatcaactc agacttaaag cgcggcccag tgttattgcc cagttcctcg cggtcttcac
    24361 ggatggcaaa cttacgcagt aatgcagtga tggcgcggcg gtcttttttg cgcataaaac
    24421 acaacaggtg ccagtgaact gtaccgtcat gatgcggctc agccacccgc acgccatacc
    24481 accgcaatcc ggctttgtgc atcgccttac gaaatgcagc aaacatgccg accagataat
    24541 cactgctttg tcttaccgtc gcatttgtcc aagttgggtt gggcctgcca ttatttagcg
    24601 tggaatggaa acgtgacgga caggtgatgg tgtagaaaac ggcgcagtca ccgcgcattt
    24661 ccgcgataag ctccaggcct ttaacacagg ccatcatctc attgcggcga tgcgcagggt
    24721 tgctgctgct ggcgtttacc acatcctcca tgtccagcgt gtcgccgtct tcgttcacca
    24781 gttcatgaga acgaaaaaac tccagcgact tacggcgctg ctcacgttta tgcatcacgg
    24841 cttcatagct gacataggga gatgcttttt tgctgaccag gcagacagca cgcaactgct
    24901 cttcccgcca ttcgcaacgc atcttccata atttccggta ccaccagtca gcgcacaaca
    24961 tacgcgccag cgaacccgga atgagttcat agggcacggg tttacggcgg tttcttttcc
    25021 ggcggagttg ctcaaacgca ggcgggatga catccagatg cagggtttcc gctgccacct
    25081 tttcccatgt cttgcggatt tcttctggct taacgtcatc ggtgacatac aaatcgccac
    25141 aagctgcatc aaggcacatg ctcatatgcg cagctaccag ggtggacagg cgtttcacct
    25201 gatcctgact catttcaggc aggatcagca ggccgtccag cccttcatgg cttgccataa
    25261 agcgaaaaga agtggatagc tgacagtcgc gtacatgctc cagccgttcc agacatggct
    25321 taatcgtctc acgcaaatag cgggaataag cctttggcct gcccaggctg ctgaagtatt
    25381 caatacgttg catcagcggc ttgctgatat gggaaggctg ggcattgacg tccgccagaa
    25441 tgaccatatc tggattaaaa cgctgctgct catgcgccag ctttgcccga ctaatgagct
    25501 tatcctgctc catttcgcgc tggacaggat cacgggattc attaaagaaa taacgctccc
    25561 agacctgatc actcagtgcc tcgcggcgca gctgttcctg ctcgttatcg gcagcgtaca
    25621 gagcgatcag gtttgaaagc gcagaaactg aagattgctc ttctgtctct acgtaaggat
    25681 tgattgcttt tttctcagca ttccaggaat agctgtagtt cattacgcaa tctccagttc
    25741 gagctgtgaa ggctgcaaac cattcgacag ccaatcagaa actgaaggtg ggcgaacagc
    25801 ttcaattgca ccttttaaaa ttgcgcaacg gtttttcaga atgacagctt tcagctcctt
    25861 ttccgtcaga ttgcgcgaat actcagcctc ctgaatagcc cgcgtcagct caggatattt
    25921 gctattaaat ttggggacat tgcaggcaag atttgtactg tcggcagtcg ccagtgggta
    25981 atttcccaac acacgaccgt caagcatgcg caaaccatga acagctgttt tgaaattgtg
    26041 tcggcaataa attgcttcaa aagcgtcctg catacgacga tgccagtgcg cagttctgat
    26101 agccgcatat tcaccagacg atccaaagca gacacgaggc cattcacgac atagctcgat
    26161 aagccgatcg attgactcgt gcagatgcca gacgggagtt gccttcccgt agaacatttt
    26221 cggaacttcg tttatcaggg catcattgtc acgttcacct ccgtccacaa catcaggaat
    26281 gacaaaaaaa gcgaccttag gatggtggta atagttcagg agccatttat aaaaatcact
    26341 ccagttaatt tttagcccac gcacccatgc agaaaatgcg ccgttatcaa tgccgacgac
    26401 ctgagcgtgc tgaatggacg ccgcaatctg atctggtcgt acataggaga cgaaagcgcc
    26461 agctccgctc accgcaatac gatgaacgtc gccagcactt ccccagacag gcgtcccatg
    26521 gaaatgatga attccgtggt gcatttcttt cacacttgca ccccgaaaat aacagtagag
    26581 tcacgtcccc tactaaaatc ggcactaaac caattagcag atttagtggc aatcatctct
    26641 gttgcaaatt ttccctcccc cgctgcaacg ccgatgctgc gtttcgccct gatgtagtgg
    26701 tgagtgaaat tacgataaag ggaccgggtc aaagacgtgt tactgttaga aacaatgacc
    26761 ggatgtcctt ctgatgaccg atgttcaaga atggatgcca ggtgatactg atcatcttca
    26821 gtgaaaccat cagtgtgata gccggaaaac gtaccgtcat aaggcggatc gcaatacacc
    26881 acatccccca ccttcaacat cgccagcgtt tcatcaaagc tggcgcagat aaacgttgcc
    26941 cgctgggctt tctctgcaaa tgcgcgaatt tctttttcag ggaaatacgg atttttataa
    27001 ttaccgtagg gaatgttgaa atgcccgctc ttgttatagc gacataaacc acggtaaccg
    27061 tgacgattga gatacaggaa atataccgct ttcatgaaat cagtaatttc agtggagtaa
    27121 ttaaactcct gccttatgtt gtaataagcc acctccctgt ttgcttcctc aaataaaact
    27181 ctggcacgag atataaacgc ctcacaatca gcagcaacct ttttatagag gttgattaaa
    27241 tcaggattaa tatccgcaac aagatagctg ggataatccg tctccatcat cactgcacag
    27301 gaacccgcga aaggttcaac cagtcgcggg ccagcaggaa gatgcttttt cagttcggac
    27361 attatggcgg ttttatttcc tgcccatttc aggatggtgc tcatacagca cctccgttgt
    27421 aatgtttgcc tttcagctct gcgatttcct gacaggtaat gcaaagctgc acacccggaa
    27481 tggcacggcg gcgtgctggc ggaattggcg cttcacactc aacgcaaagc acgcgggaca
    27541 cgcccggcgt tttggcacgg gcagcacgga tatggcgttg gcgttcttct tcaacgcgct
    27601 gctgtacgag atccattgca tcagccatca gtggatctcc tgcgcttcgt tctggattgc
    27661 ttcagcagtc acacgcagca gttctgccgc ttcgacgtgg tttagctggc gggaggtgat
    27721 atgacacgcc aggctatcga ggcgagcagc cattgcttca gcccttgccc ggcgttcttc
    27781 cagacgagcc tctgtcagta aaagattaag acctgcatca tccggtccgg ttttggtcgt
    27841 gagggtttca atattacgca taagcaattc tcctgaattt agataaaggg atgcccggcg
    27901 ggtttacgcc attaatttca ttagttggtt aattcggcat ggttagccgt ctgggaaata
    27961 agctcaccac tgcacgaaaa tgattcattg ctttaatcaa ctcccgcttt tcgtcagtgg
    28021 tcagctcatt aatgctgatg ctatgacgtt cagctggaat ttttgccata aagaatatgg
    28081 cagccagtgc ccgtttattt tgttcattat tgatatcccg aggatcacgc atatctttaa
    28141 taaaccgctc aagctctgac tcaatattca aaccaaaaac tttcgccctt aactccgcaa
    28201 tatgattaag tccattcagg cgttcaccgg gtcttaatgg aacagtcgcc gcagcgcctt
    28261 caatagccat ttgttccccc gttttttcgt agatagttct gccagcaatt catcttgtga
    28321 acggcacgga tgccagcgtt taccatcctc acccatgatc cagccgtgac cgtagtgcat
    28381 tgccggactt tgttttacca gcagcgatgc aaatgatggt tctttcgtca gcataagcac
    28441 ctcacagcaa accgaatgaa gcaccgaggc cagtcacggt atcaactgca ctcgccatcg
    28501 caggattagc ctgtaaacgg gcctgcaatg aaacagcagc cagcgccatc agtcgtgtaa
    28561 cagagttaat gctgctgatc gcatcacgac ggcctgcact ggtttttaca tcgccagaaa
    28621 ccgcacctgc cgcgacacgc cctatctctg cagttgcact catgacgtaa tgcggcagtt
    28681 tctcttttgc cacctcatta atcggtacac atggcaggca gtgaatctgt gccagaaagc
    28741 catctaccag cgttgaatct tcagtcagat cggtaagcaa ccagatttct ggtgcggtta
    28801 ataaatgagg ttgagctggg ttcagcttgt tccgcagaat ctgcacattc atgcctgcac
    28861 gttctgccag ttgcactagg ttgtggcgca atgcgaatgc acgacaggct tcatcaaaat
    28921 gtggatgttt ggaaacttgg taatcaaaca tggtcaatgc ctctgatgta tttcagaatc
    28981 gaactaatta aggtttagat tgcattctga aagcgcatca acggtcatgg ctgctatgtt
    29041 gatcatcact ttttcacgtt ttttatcttt gcgcagacgg tgacggataa ggcgtccgtc
    29101 agccaacatg tcattgatgg tatcgatgga cagccctgtc agctcgctat agcgttcaat
    29161 agtcacatga ggcgtggtaa gagtgattga aatgttaggt ctcatgatgc aacattcctc
    29221 gtttaatgat gattaatcag gacgaatacg gatcgtttgt attttgtgaa caccataaac
    29281 atacgatcgc acagtgaaat cgtcaagata aaagttcact tggagtgacc atgaatttgg
    29341 agaaaggcgg acgaggcgct atagagcgca tggtagaagc ttatggattc aaaactcgac
    29401 aggcgttgtg cgatcattta ggaatctcta aaagtacact cgccacacgc tacatgcgtg
    29461 actcattccc agcagaatgg gtaatccagt gcgcccttga aacgggcacc tcgcttaatt
    29521 ggctcacaac cggacatggt tcaaagcaaa cttcaggtaa tacaaatact atggaagttg
    29581 ctaaatatgt attatctgat ggtgccttgc gtgaagacgg tttttatatt tttgataagg
    29641 gatttctacc ctctacgttt aaaaaacctt ttgtcatcac agataacaat tctgaattta
    29701 tttgtgataa agaatttgat gatatacgtg acggtaaatg ggtaataagt attgatggcg
    29761 aaataacaat ccgcgacatt actcgtttac ccggtggaag aatcttcgtt gaaggtggaa
    29821 acagagcctt cgagtgcaag atagaagatg ttgaaataat tgggaaaatt ataagtttaa
    29881 caattaagta cgttaggtaa taccgggagg aaactatgct tggtaaggta ttttttgtgg
    29941 ttttatcatg ctctttgtta ttaaacccac taactaccta tgctaaaaat tatccttgtt
    30001 ctgggaaaaa gggaggtgtc tctcactgta cctccgatgg aaagttcgtt tgcaatgatg
    30061 gaactattag taaatcaaaa aaaatctgta ctaaaaactc gcgataaatt ttgcttttat
    30121 atctgcgcct aatataacaa tgagccgcag gctaaccgca aaagtcacat actcacatag
    30181 caaaaaatag ccaacttcat tatggcttca gtgagatgta tggtcgcagg atttcataca
    30241 ttgacactgg ttatacatac agtaaaaatg ctctctattg gagggcattt tttatggcag
    30301 tacgaaaact caccacagga aaatggcttt gcgaatgtta ccccgccgga cgtagtgggc
    30361 gtcgtgtgcg taaacaattc gccaccaaag gcgaagctct ggcttttgag cgccatacga
    30421 tggaagaaac cgaagcaaag ccctggctgg gagaatcagt ggatcgtcga acactgaaag
    30481 acgtggttga gctatggttc aaactacatg gtaaatctct aaccgctggg cagcatgtct
    30541 atgataaatt gctgttgatg gttgacgctc tgggcaatcc ccttgcaacc gatctcacct
    30601 ctaaaatgtt tgcccactat cgagataaac gcctgacagg tgagatctac ttcagcgaga
    30661 aatggaagaa aggagcaagc ccggtcacca ttaacctgga gcaaagctat ctaagtagtg
    30721 tttttagcga actatcccgc ctgggcgaat ggtcgtatcc aaacccactg gagaacatgc
    30781 gaaaattcac catcgcagaa aaagagatgg catggcttac ccatgagcag attgttgaac
    30841 tgctggctga ttgcaaacgt caggacccaa ttctggcact ggtagtcaag atatgcttaa
    30901 gcacaggcgc acgctggcga gaagcaataa accttacccg atcacaggtg actaaatacc
    30961 gaattacctt tgtaagaacg aaggggaaga aaaacagaag catccctatc agtaaagagc
    31021 tttatgaaga gatcatggcg cttgatgggt tcaatttctt cacagactgc tattttcaat
    31081 ttttatccgt gatggaaaaa acgtcaatcg tgctccctcg cggtcaactg acacacgttc
    31141 tgcgccatac gtttgcggcg catttcatga tgtcgggtgg aaatatcctt gctttgcaaa
    31201 aaatcctcgg acatcacgat ataaaaatga ctatgcgtta cgctcatctg gcaccggatc
    31261 atctggaaac ggcgctccgt ttcaatcctc tggcaacgct gccaagtggc gacaaagtgg
    31321 cggcagcggt tggcattacc ccgtaataac caccactgac caccaaccta acttattgtt
    31381 tttaatctaa cttattgttt tcattaaccc gtttacataa atgggttttt tgttatctaa
    31441 aatttatcta ctaccctgca accctctcaa ccatcctcaa aatctcctcg cgcgatagcg
    31501 gcttacggtc ggtgacaaaa tgcagcgtca tcccctcaat aaacgcatca agcgcgcggg
    31561 cggttccggg ttcaaaccat tgttcgagcg tttgctgact gcgctgcatc cagttttgca
    31621 ttaccgtttt taatagcggt tttcggctag ccagcgcgta gagctggtac atcagctcca
    31681 tgttatccgg cgttgcaacc tgtgagctgt agatcatatc ggtgatagcc tggcatgcgc
    31741 cttgagcatc actaacatcg ctaaaaaatg cctgatattg ccgggacata atctcagtaa
    31801 aactgctgaa cgcctccagc aacaactcat caattcctga aaaatagtag gtcatcgatc
    31861 ccaacggtac cccggcaagg gtagcaattt tgcggtgcgt aacagcatgt attccgtaaa
    31921 gtttcaccgc ctccagcgtg gcctggataa ttttttctcg ccgttgcgga tcgttagcgc
    31981 gacgcatgtc atttcctctg tctgcaattg tgtacaaatg tacacaacct tgctaatgtt
    32041 gtcttccctc ttcttatttt gacgtgatct atgaccgtaa attcttcacg taatgcattg
    32101 aaacgccgaa cttgggcgct gtttatgttc ttctttttgc caggcctgtt aatggcgtcc
    32161 tgggcaaccc gtacgcctgc tatccgcgat attctctctg tctcgatcgc tgaaatgggc
    32221 ggtgttctct ttggtctgtc gatcggttcg atgagcggta ttctctgctc ggcgtggtta
    32281 gtgaaacgct ttgggacacg taatgtcatc ctggtcacga tgtcctgcgc attgatcggg
    32341 atgatgatat taagtctggc actctggctg acatcgcccc tgctctttgc cgttggtctc
    32401 ggcgtctttg gggcaagttt tggttctgcg gaagtggcga taaacgttga aggtgccgcc
    32461 gttgagcgag aaatgaataa aacggttttg ccgatgatgc acggttttta tagcctgggc
    32521 acgctggcag gcgctggtgt cgggatggca ctgacggcct ttggcgttcc ggcaacggtg
    32581 cacatttcat tggcggcgct ggtaggtatc gcgcctattt atatcgccat tcaggcaatc
    32641 cctgacggta cgggcaaaaa tgctgccgat ggcacccagc atggcgaaaa aggcattcct
    32701 ttttatcgcg atatccagtt gctgttgatt ggtgttgtag tgctggcgat ggcctttgcc
    32761 gaaggttctg ccaacgactg gttaccctta ttaatggttg acggtcacgg ttttagccct
    32821 acttccggct cgctgattta tgccggtttt accctgggga tgaccgttgg acgcttcacc
    32881 ggtggttggt tcatcgaccg ttacagtcgc gttgccgtgg ttcgggccag tgcactaatg
    32941 ggggcgttgg gtattgggct gattattttt gtcgatagcg cctgggtcgc aggagtgtct
    33001 gttgtacttt ggggactggg tgcctcgctg ggcttcccgc tgaccatttc tgccgccagc
    33061 gataccggcc ccgatgcacc aacccgcgtc agtgtggtag caacgaccgg ttatctggct
    33121 ttcctcgtcg ggccgccgct gctgggctat ctcggcgaac attatggatt acgtagtgca
    33181 atgctggttg tactggcgct ggttattctc gcggctattg tcgcgaaagc cgtcgccaaa
    33241 cccgatacca aaacgcagac ggcgatggag aatagttgat gagcattaaa ttaattgcgg
    33301 tagacatgga tggtactttc ttaagcgatc aaaaaaccta taaccgtgag cggtttatgg
    33361 ctcagtatca gcaaatgaaa gcacaaggca ttcgctttgt ggtcgccagc gggaatcaat
    33421 attatcagtt gatctctttc ttccctgaaa ttgcgaatga aattgccttt gtggctgaaa
    33481 acggcggctg ggtagtgagt gaaggcaaag atgtttttaa tggcgagctg tcgaaggatg
    33541 cctttactac tgtcgtggaa catttgctga cgcgcccgga agtggaaatt attgcctgcg
    33601 gaaaaaatag cgcctatacg ctcaaaaaat atgacgatgc catgaaaacg gtggcggaaa
    33661 tgtattatca ccgtctggaa tacgtcgata actttgacaa cttagaagat attttcttta
    33721 agtttggcct gaatctttcc gatgaactga ttccacaagt acaaaaagca ttacatgagg
    33781 ccatcggcga tattatggtg ccagtccaca ccggcaacgg cagcatcgat ctgattatcc
    33841 ccggcgtaca taaagccaat ggccttcgcc aactgcagaa attatgggga atagacgaca
    33901 gcgaagtggt ggtctttggc gatggcggta acgatattga gatgctgcgt caggcaggct
    33961 ttagttttgc aatggaaaat gccggcagcg cggtcgtcgc agcggcaaaa taccgggcag
    34021 gctccaataa ccgtgaaggc gtactggatg tgatcgataa agtgcttaaa cacgaagcgc
    34081 catttgacca ataaaaacgg cagcacgccc ggcaacacaa tacctgaatg aaggctgcat
    34141 aattcgcgga aattaacccg ctgcattatt tcaggcgaaa tgattatgaa aaattgtcta
    34201 ctgttgggcg cgcttttaat gggctttact ggtgtggcga tggcgcaaag tgtcaccgtc
    34261 gatgtgccga gtggatacaa agtggttgta gttcctgatt cagtcagcgt tccacaagcg
    34321 gtcagcgtcg cgactgtgcc gcaaaccgtc tatgtggctc ctgcgccagc tccggcttac
    34381 cgcccgcatc cttatgctcg ccatcttgcc agcgtaggtg aagggatggt tatcgaacac
    34441 cagattgacg accatcacca ttaattcgag aatgcctgat cgcacaaagc agtcaggcat
    34501 ttttttaccc ttcgtgagaa tttcccatct gtttatcttt taaaaagata accatcagcg
    34561 acagccacaa aattccgttg acaaggttga agagattaaa ctgtccgttg cccccgttca
    34621 gccaggcatg tttgctgatt tcaataccaa cggtaaagat cagcatttgc agcattccca
    34681 tcgccgcaga aaccgtacct ttactcatat cgctggcaaa cagggttaat cgcaccagtc
    34741 ccgcattcgc cagaccaata ccgaaagcat aaatacttaa cccggcagtc atccataaat
    34801 acgcgtgcga tgagataacc gttgccgcag cagcgaccaa cagaccaatc ataatcggcc
    34861 agccgcccat aattatcagc gaacgtacgg tgcggcgcga ggtcaaacgc gctaacaaca
    34921 agttacccgc aattaacgcc ccgaaaatag gcacttgcag caagccatat tcatagctgc
    34981 tcaactgctc gccggtaatg atgataatcg gcgactgggc gatccacgcc agcaatggca
    35041 ggctaacgaa tcccagcgcc agcgcccccg ccacaaagcg gccgttcttc agcaccagct
    35101 tatagtcacg accgagttct ttcagtgaca gtttctcgcc tatacgcgtg gcggtttcag
    35161 gcatagctcg ttgcagaccg aaaaaggaga tcgctgccaa tgcggcaaac aaaacaaaca
    35221 tcccctccca gggcagcaca tggatccacg ccgcgcccac cagcggacca agtagcggag
    35281 caatcagcgc cacgttcgcc atcagcgcgg tgatcttgat acaaaccgcc tcttcgaagg
    35341 attcctgaat tgcggcgtat cccacagcgc caatgaaaca gaggcttatg ccctgcaaga
    35401 agcgcaacag ggtgaattgt tcaatgtttt gcgccagcaa tattgccaga caggtgatga
    35461 taaaccacac cacccccgcc agcatcaccg gacggcgacc aatacgatcc gacagcggcc
    35521 ccaggagcca ttgtaaaaac atcccgcccg ccagatatgc ggtcattgaa gtaggaaccc
    35581 aatcaatgcc cgcctgatat tgttccacca cggccaacat accgggttga atcatatcgt
    35641 tgccgatata ggttgaaaat tcgtaaagca ccagacagag agggaaaagt aacgcctgac
    35701 gtccaagcct ggcaccggaa gctaatttat tttgcatgca atttcttcgc caataataat
    35761 cgcgcagagt ttaataaaag cacagctaac gagaaagcga attttgtagc tgaaaccacg
    35821 gttaagcaca ttcttacatt attgcgagta tagctacgct ttctttaagt tttatttaac
    35881 ctatgcccgt tacaattacc caccgtaaac aggccgcttg agggaaataa gacgatgccg
    35941 ctttacccag tttaacctgc actttattct caacgacttg cctgtattgg ctccctttta
    36001 atcactttgc gtcgggaagt tatgctggaa aatttgaatc tctctctatt ctctcttatt
    36061 aacgcgacgc cagactcggc tccgtggatg atctcgttgg cgatttttat tgctaaagat
    36121 ttgattaccg tggtaccgct gctggccgcg gtgctttggt tgtgggggtt tacagcacaa
    36181 cggcaactgg tgataaaaat cgctatcgcg ctggcggtca gcctgtttgt gtcctggacg
    36241 atgggacatc tttttccgca cgaccgaccc tttgtcgaaa atatcggcta taacttcctg
    36301 catcatgcgg cggatgactc attcccaagc gatcacggta cggtgatttt cacctttgca
    36361 ctggcatttt tatgctggca tcgcctgtgg tccggctcac ttttaatggt gctggccgtc
    36421 gtcattgcct ggtcgcgcgt ttatcttggc gtccactggc cgctggatat gctcggtgga
    36481 ttgctggcag gtatgattgg ctgccttagt gcccagatta tctggcaagc gatggggcat
    36541 aaactctatc aacgtctgca atcgtggtat cgcttctgtt ttgcattacc gatccgcaaa
    36601 ggctgggtgc gtgactgatc tctgctaaaa agtgtagtat tgagcggctc gcttcaataa
    36661 ctattcagag ggattatgga aacacgtc
//
LOCUS       DAMZET010000027        36348 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.027, whole genome shotgun sequence.
ACCESSION   DAMZET010000027 DAMZET010000000
VERSION     DAMZET010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.027"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(14..850)
                     /locus_tag="POB34_001471"
     CDS             complement(14..850)
                     /locus_tag="POB34_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI5918450.1"
                     /translation="MQRSWFNNRLTSAKQKSLLYKSLADLVQSMMDTFVDPWLERITN
                     RKSIFSMSKEDLETRTNELGQFFTIRTSNSSSVPMLLQQRLDEIHFKGTERPINQTIY
                     REFNGISVLWDPIYAPVDLERHPYGTVLIPESTLETTGGTFGEMFLTSRGMISIPIND
                     LARTMGITGTIDQSAITEEILRKFNQFVKPLLPLHIVFDGLTLYLSVVVNEHADMITL
                     NEISDTEKAYCWFETSDTTSLTGVTSISAPITATPGGTIVKATPTFDRTRADDLLLDS
                     DA"
     gene            complement(850..2283)
                     /locus_tag="POB34_001472"
     CDS             complement(850..2283)
                     /locus_tag="POB34_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin hydrolase"
                     /protein_id="HDI5918451.1"
                     /translation="MSKTTPTKDSIRAEFEELVEKDSFWSKFVGSQFVSMLTLFITQI
                     VYRCFQYADAALAEGFISTATRRSSILAAAETNSYVGTKPTPSSGMIEITATSEDAPA
                     VIPKNMPLISDDQYPYMTMDVCRLVDGTGTVEVAQLEIQEVTYTVTAAKEFLEVVLSK
                     ALTAVCYKLEVFVTTDGKTTQWSSSTMFRLAGSKSQVYVEFYKPSEQLGVRFGDGLIG
                     QIPPEGSTITLKVWCTSGDITLVAGQNLTPVDSATNLANLISVKTTTPITAGTDAETT
                     EITRNRAQYYLAYDDQVVWGGDYTYFLVRNIPGLSWVKAWGEGQQEKLDGAYNVQNIN
                     KIFISGWHPNKSQSELEEMILAAFKKVPNELNKKFSYKEVRKLPFKITITGRISASLT
                     IENVTDELKSALETKFGRDSTFFDPNRVGKYILIKKKDVWAFIETLGYFRDFYLEFVE
                     WNESNGFYDFVYLDTENSTFNISYEEE"
     gene            complement(2280..2636)
                     /locus_tag="POB34_001473"
     CDS             complement(2280..2636)
                     /locus_tag="POB34_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918452.1"
                     /translation="MANNNEIDPLLTLELSGVKTYESQEEAWGARLYEWLNTYQGEVY
                     GDPSWGNVLPQFKHEPTNLSHVQIAVEAMLLQKLTVDLPDIPISGLSVAEGDAFDKLK
                     ISIRIRDITITQDVVL"
     gene            complement(2636..6058)
                     /locus_tag="POB34_001474"
     CDS             complement(2636..6058)
                     /locus_tag="POB34_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HDI5918453.1"
                     /translation="MERKNANIDDVIRTVETASAKELEELAGIREAVEDLKGGRVATV
                     DPVSRSVSALNRTIENSRPDFVTNAPSVDPIVDAMKRLNLGDVSRVVQEGIAQQEQQD
                     KSTTPKGKKRRRKAIPEDIKAQRTEAAEHAREMFDQKGGAQKSQNQRDARGRFIGKSG
                     SKAAAEDARAERAEKARRKEDDERLNVESGLLKKLSKVAEGIGNSSETRAVDALGYAV
                     AGPLWAAGKELGGISKEVGGSLNGARKSIADVIRGNDDNSRRKGFFRRKSQNSADVVQ
                     VNTQKRTVQELQEQTSEIKEGNDKILSALDQIAKNTGKKKGGLLSKLFSLLGKGAGGV
                     ASLLMGHGMLKKAGALAFGALGAKKLVGMLRGGGKKTLAHEGGDLAARAAGKLGLKAV
                     GKGALRAIPLVGTVAGGIYDAVTGWNDTEAQRRAFGLKSGQDPSFQQKAAYTLANVLD
                     MGGLVSGISSAIGEVLKSLGFEDIGNMLQSFSTESIAQAIDSGITNLETYISNLGDTI
                     STKFEDYTAKIGDAVSAWFSDTSNKLLEKLDAIKDFFTVDNLKQVFSDAIDSAIDFIK
                     NPGKHIKEAAGNIWDGVKNLPGKALDAAVDAVKNTPAAMIVSKIPNPIGEANAKEITP
                     ELKAPVNSHQETSDSKTESDAKQTNIVTRVINAALDTAKDSNKTVKETANQIINANAV
                     ETGNSALQKIDNAIGQNSSSSSSLNTTGTRNDIQKAADTYNNGNLDVKVGNLGAEGKA
                     NLDKLAPYFAELENKYGLPEGTLYSIAATESGGDPNAKSPLTRSPDGKLSGGALGMFQ
                     FTSIARKETGISEPDAFDPVKSAEAAALLMSKYLKQANGDLNEAITAYNAGFGTINKW
                     KKGTGDLSKENREYAIKVNTHRARYLGGDIYTPGAGAQGGAQYGVRGPLPDNAVIDQS
                     TGLAFTPGDSPFEKGGLVDKIGNAVGVNDLVNKFMNGRGMRREVVQGTLEERARGKGT
                     ATAAGNVYVDTPMPVEEARPVASNSSYFDQLGAQMGIDGLFDKLRNSPGMRKNNAPEP
                     ASTSQVTTATNDLQQPTGRMQIDGQVISDLGGSGAKPTMQLADNTVSLDGETKRLFAQ
                     MTSLLARIEEHTKDSAKGQGTVVKVSTPQPGVMRTVPLSIDDPLMNDYARVD"
     gene            complement(6140..7021)
                     /locus_tag="POB34_001475"
     CDS             complement(6140..7021)
                     /locus_tag="POB34_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="morphogenetic protein"
                     /protein_id="HDI5918454.1"
                     /translation="MLLPLFPLPSRPTELIQFRQPNIADAMRFNSITPEEQEQQTTAY
                     LKALLAEPAKYDPLTWTAQDRITALWWIFTGSRETPVETFTYTCKHCGKEHYYDCDMN
                     ALAEDIQVLEVEPFIDDIEVSVEGVPYQWRIVPLDGWAMEMLEMRRAALPPEDDAEFK
                     EAIVDLRFWEFAYQCELYNDVSGTREDQAERRYETIKRMAIDTEFMKLAAHIRLAHEK
                     LEHGLPCYIDKGEMRLRLPPHKCPNQDKKESTEGAYTRLWVPFRATDFIPQVGIEKLS
                     DLSVQPGFVWGYTDSGR"
     gene            complement(7036..7647)
                     /locus_tag="POB34_001476"
     CDS             complement(7036..7647)
                     /locus_tag="POB34_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI5918455.1"
                     /translation="MGLNVASVKSYVSSALTTTLFGSGVGEREVGKLTSIIMNKMLFA
                     QGWQFSVEVDGLEGADFFAKDITYHDYSIEYETIKIGGGNILQPTERSPGQITMMVRD
                     TVDGLVLDWFKTAKGRVINPDGTGNIPSKYLLNVRIYRLLSSGLTKLENEMTVFPVTT
                     GDVTYARDQVTEFKSFPMTFALHSTFNQSSSSLASLLGFSFSL"
     gene            complement(7658..8224)
                     /locus_tag="POB34_001477"
     CDS             complement(7658..8224)
                     /locus_tag="POB34_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918456.1"
                     /translation="MTPRQLLEDVKSRFTPLIADEPALLESLLRKALGTYQDRAGHIK
                     RIRFTDQTCKSLACPADFLALVSVTDHTGDLVYSDVYDGNIELEDTHRAVYPLNVSFL
                     ANLRDMDLDNGEVPPEIIGLLSDYLEVLIAIPNTDRLRRISIAGKLDASNLSDENTLY
                     QRKLDLEEKMSATRAIIPGIVLFSSMLK"
     gene            complement(8305..8844)
                     /locus_tag="POB34_001478"
     CDS             complement(8305..8844)
                     /locus_tag="POB34_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HDI5918457.1"
                     /translation="MNKIILFLIFSTFSVGTALANSLQSQIAAIAQAENEGRAKEQQA
                     EDARKELIRQQAQAERIRREKAASAAAAREKQRVAAENERRAKREAELANDKKRDQAY
                     EDELRKLQLESMKLELQAKAARVQRENDFIEQELKERAAKTDVIQSEADANRNISTGS
                     KDLLQSEGKAREKKASSWW"
     gene            complement(8848..9360)
                     /locus_tag="POB34_001479"
     CDS             complement(8848..9360)
                     /locus_tag="POB34_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918458.1"
                     /translation="MSKKYTLCALVVSAILLSGCQSSGADYAADVYDTAQLNSKQETK
                     TVNIISVLPAKVKVDNKANKEAAQTFGAVLGAVAGGVAGYNVKGTSTLGAVAGGTGGA
                     ALGAAAGSLVSDKTIVEGVSLTYKEGTKVFTSTQVGKACQFTTGLAVLISTKDNETRI
                     QPNATCPEKK"
     gene            9982..10203
                     /locus_tag="POB34_001480"
     CDS             9982..10203
                     /locus_tag="POB34_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HDI5918459.1"
                     /translation="MVNANPCARQEFIWRFYSCKKHHYHFVIAATEDEARSQLPDGPC
                     IFTARFSTNSRNSLSYWNLPFSSDVQGVL"
     gene            10200..11243
                     /locus_tag="POB34_001481"
     CDS             10200..11243
                     /locus_tag="POB34_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDI5918460.1"
                     /translation="MKKPLVTRNDIAEAIALHTACMPTREIPGAIANYFMITRRFYTR
                     TDKAVINRLLIAEIRDYLIEQGRLRYATVAAEMRKEAHRMTSNNLNVKKPASFASATP
                     SPAVNVISNTGDTIDSQTLLKMVNEARKLCGEPEVRNNKFIKKILDELEGEDGYTKSA
                     TVPPGGGTPMVVITMTYKQALRVAARESKAVRRSLIDKLEELQQANSPAPSIPQTLPD
                     ALRLAAELAEQKMQLEQQLVAAAPKVDFADRVSAANGILIGNFAKVVGLKQNALFSWL
                     RQNGILMAFGARKNVPRQQYINAGYFTVKEVVLDDENGYQIRLTPQLTGKGQQWLTRK
                     LLDAGLLKPVAIG"
     gene            11407..12207
                     /locus_tag="POB34_001482"
     CDS             11407..12207
                     /locus_tag="POB34_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001187871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDI5918461.1"
                     /translation="MQQTFNADMNISNLHQNVDPSTTLPVICGVEITTDRAGRYNLNA
                     LHRASGLGAHKAPAQWLRTLSAKQLIEELEKETMQNCIVSFEGRGGGTFAHELLAVEY
                     AGWISPAFRLKVNQTFIDYRAGRLQPAIPQSLPEALRLAADLAEQKQRLEQKMLMDAP
                     KVEFAERVATASGVLIGNYAKVLGLGQNYLFTWLRDNGILIATGERRNVPKQEYISRG
                     YFTLKETVIDTSNGSRISFTTRITGKGQQWLMKRLLDAGVLVHVAATR"
     gene            12237..13082
                     /locus_tag="POB34_001483"
     CDS             12237..13082
                     /locus_tag="POB34_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein repL"
                     /protein_id="HDI5918462.1"
                     /translation="MLAKVTFLSCITMSDFTFSGYELACFVTHSGLSRSTGHILSQCA
                     NLAATTSEYFIHKPHRLIAAETGYSQSTVVRAFREAVNKGILSVEIVIGDHRERRANL
                     YRFTPSFLAFAQQAKNALIESKLKISSAATKVKAVLAKTLALFNFLSTPPCQNDTPSP
                     CQDDVAIKNKKSQVKKTKRSVSGGAGTSRLKKLTSWIAEAKAKADNLRLSKKRAQKHE
                     FKQKVEAAARKYAYLKNKRSPDIGGVSNFDNLPHCMTVNEALNAVLAKNKDNEQWGIP
                     AGFRG"
     gene            13133..13378
                     /locus_tag="POB34_001484"
     CDS             13133..13378
                     /locus_tag="POB34_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918463.1"
                     /translation="MQSDFLQLAIAFAGYVCIGFCVYMISRKMLVDIDRKEQAEEILE
                     WIFFGAVWPLGIMFAATFLLMWIFTLPGDFYRKKARH"
     gene            complement(13561..14157)
                     /locus_tag="POB34_001485"
     CDS             complement(13561..14157)
                     /locus_tag="POB34_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918464.1"
                     /translation="MAVIPMSYSPGTVARRFSILDGVTIQGVLYQVIWDSKTPFAAVI
                     EAAPSIIDGDMRHKVVATLELQRRPQLEGVLVRKFWEDSDVAQIEGIVVDGTVRDVGL
                     ATFVYETVASKAGVVLLSDNEQYEGGKALWQHIARRSSELKVFILDTDAAQYYPFDGE
                     RVCYDGVSIPESEIWSEHPDRSKHGVVLVAESIIGNAA"
     gene            complement(14329..14838)
                     /locus_tag="POB34_001486"
     CDS             complement(14329..14838)
                     /locus_tag="POB34_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HDI5918465.1"
                     /translation="MGHNNTKGNRKFIKGRYTANAAKGERLVSSEFQLTFAGYEDISV
                     LVRTSQIPEMTREDVEDYGPNGVKFNQHGPIRNSGEIQVQCVETIEGDILQFIKDRIA
                     AKDYVDITMAATPESKSSGVNAVTKAATTIEMLDCKIYSDAIDFSTEDVTAAVRPSLR
                     IVYNWIEWD"
     gene            complement(14850..15431)
                     /locus_tag="POB34_001487"
     CDS             complement(14850..15431)
                     /locus_tag="POB34_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918466.1"
                     /translation="MAPIAYGVYSQADGVSPYLKVTLTNSQYQVTGYISQGAAMNMAQ
                     NWEAPFTGMSMGSVSGALGGFVQVGTETTSVARWNSLMVWEGGTPPTFTLPVTFIALN
                     NPFIEVSGAIAALTAMISPELKAANVGGRIPERVTLNIGRRINITDVAIQDLSFDLDA
                     PRDSNGYFLKNTVNLQLTGSSIYNSSDIVRAFQ"
     gene            complement(15467..16282)
                     /locus_tag="POB34_001488"
     CDS             complement(15467..16282)
                     /locus_tag="POB34_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918467.1"
                     /translation="MSKNFFQSGAFLGNGLSRFALNSDPVQLMESARASAEPPTDPVI
                     NNNPEPAAQTNDNVPSAPAPEQILEGKDGKEWTVEQAHQMILEAANRSAMQNALSDAA
                     DAVFSWADSGDLTFDSLDGFVQAIAGISDDDDSEVTEEQDDAYNEAWANVADFLAACG
                     VDDDLIEALADDEDDDAAADVGASIAGLDSDDRDELEAAFVVAGTSDEMLTEAFKKVV
                     RNGEIKLIRKRLRKKRLTAAQKSALKKARRKAQTGAAKLARKKSMKLRRKRLG"
     gene            complement(16292..17881)
                     /locus_tag="POB34_001489"
     CDS             complement(16292..17881)
                     /locus_tag="POB34_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918468.1"
                     /translation="MSQYSIQQSLGNASGVAVSPINADATLSTGVALNSSLWAGIGVF
                     ARGKPFTVLAVTESNYEDVLGEPLKPSSGSQFEPIRHVYEAIQQTSGYVVRAVPDDAK
                     FPIIMFDESGEPAYSALPYGSEIELDSGEAFAIYVDDGDPCISPTRELTIETATADSA
                     GNERFLLKLTQTTSLGVVTTLETHTVSLAEEAKDDMGRLCYLPTALEARSKYLRAVVN
                     EELISTAKVTNKKSLAFTGGTNGDQSKISTAAYLRAVKVLNNAPYMYTAVLGLGCYDN
                     AAITALGNICSDRLIDGFFDVKPTLTYTEALSAVEDTGLLGTDYVSCAVYHFPFSCKD
                     KWTQSRVVFGLSGAAYAAKARGVKKNSDVGGWHYSPAGEERAVIARASIQPLYPEDTP
                     DEEAMVKGRLNKVSVGTSGQMIIDDALTCCTQDNYLHFQHVPSLMNAISRFFVQLARQ
                     MKHSPDGITAAGLTKGMTKLLDRFVASGALVAPRDPDADGTEPYVLKVTQAEFDKWEV
                     VWACCPTGVARRIQGVPLLIK"
     gene            complement(17942..19648)
                     /locus_tag="POB34_001490"
     CDS             complement(17942..19648)
                     /locus_tag="POB34_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918469.1"
                     /translation="MSNLREYQNRIADIAKRSKAVLGWASTAQFGTDNQFIKDDAARA
                     ASILEAARKDPVFAGISDNATAQIATAWASALADYAAAHKSMPRPEILASCHQTLENC
                     LIESTRNSMDATNKAMLESVAAEMMSVSDGVMRLPLFLAMILPVQLGAATADACTFIP
                     VTRDQSDIYEVFNVAGSSFGSYAAGDVLDMQSVGVYSQLRRRYVLVASSDGTSKTATF
                     KMEDFEGQNVPIRKGRTNIYVNRIKSVVDNGSGSLLHSFTNAAGEQITVTCSLNYNIG
                     QIALSFSKAPDKGTEIAIETEINIEAAPELIPLINHEMKKYTLFPSQFVIAAEHTVQA
                     AYEAQREFGLDLGSLQFRTLKEYLSHEQDMLRLRIMIWRTLANDTFDIALPVNQSFDV
                     WATIIRGKFQTVYRDIIERVKSSGAMGMFAGADAASFFKQLPKDFFQPAEDYIQTPYV
                     HYIGTLFGNVKVYEVPAGICKNLTTENIQFSSMDVLCYVRDENPGKAGFVTGDAVPAI
                     PFQHPTTPALVNRTTLWGSAINDMHPRNGADYFTRVTLTMAKKGGLNFISGDTIDAGD
                     SE"
     gene            complement(19874..20875)
                     /locus_tag="POB34_001491"
     CDS             complement(19874..20875)
                     /locus_tag="POB34_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDI5918470.1"
                     /translation="MSKKNRPTIGRTLNPSILSGFDSSSASGDRVEQVFKLSTGRQAT
                     FIEEVIPPNQVESDTFVDQHNNGRDQASLTPKSLKSIRSTIKHQQFYPAIGVRRATGK
                     IEILDGSRRRASAILENVGLRVLVTDQEISVQEAQNLAKDVQTALQHSIREIGLRLMR
                     MKNDGMSQKDIAAKEGLSQAKVTRALQAASAPEELVALFPVQSELTFSDYKTLCAVGD
                     EMGNKNLEFDQLIQNISPEINDILSIEEMAEDEVKNKILRLITKEASLLTDKGSKDKS
                     VVTELWKFEDKDRFARKRVKGRAFSYEFNRLSKELQEELDRMIGHILRKSLDKKPKP"
     gene            complement(20892..22088)
                     /locus_tag="POB34_001492"
     CDS             complement(20892..22088)
                     /locus_tag="POB34_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI5918471.1"
                     /translation="MSDSSQLHKVAQRANRMLNVLTEQVQLQKDELHANEFYQVYAKA
                     ALAKLPLLTRANVDYAVSEMEEKGYVFDKRPAGSSMKYAMSIQNIIDIYEHRGVPKYR
                     DRYSEAYVIFISNLKGGVSKTVSTVSLAHAMRAHPHLLMEDLRILVIDLDPQSSATMF
                     LSHKHSIGIVNATSAQAMLQNVSREELLEEFIVPSVVPGVDVMPASIDDAFIASDWRE
                     LCNEHLPGQNIHAVLKENVIDKLKSDYDFILVDSGPHLDAFLKNALASANILFTPLPP
                     ATVDFHSSLKYVARLPELVKLISDEGCECQLATNIGFMSKLSNKADHKYCHSLAKEVF
                     GGDMLDVVLPRLDGFERCGESFDTVISANPATYVGSADALKNARIAAEDFAKAVFDRI
                     EFIRSN"
     gene            complement(22257..23066)
                     /locus_tag="POB34_001493"
     CDS             complement(22257..23066)
                     /locus_tag="POB34_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDI5918472.1"
                     /translation="MQEEKQHYLYVLVPENGDTFKIGISCGPLARFKGLQVSPDFALS
                     RVYRGTRLAMVNLERALHATFFPWNAPWEKSVGGGHTEWFTRECLDKVLAHIEYLNDM
                     WGGILERIKSNDLLQRPVDAACSFEKELDVTSIVTFKDDAGMRDVAYVSISGYEPDAI
                     RAQCELLKAMFMLRTKYPWDTGRVCFPMEELTATIDSQLYHDNPEKFFSLLAGNGLNC
                     VSGLGRSRIQHASLFGPFFYDRHGYFEAELPALTRAIDTIDFERLFAALSK"
     gene            complement(23359..24243)
                     /locus_tag="POB34_001494"
     CDS             complement(23359..24243)
                     /locus_tag="POB34_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDI5918473.1"
                     /translation="MPEESKGFLSVEEVAGNTGEIHSLKPNNNSTIQPIALLRLGVFV
                     PTLKSTNVALRRGSSVTTNTTNATEELSSLKIVEQEGYEGIEIHGPRLDMDTDFKVWV
                     GITSALFDYAPDDDGIITLPFSEFADRCGYPRKRLSKAFRKSIDDSLTRIQQTVVKFR
                     FPAAKGHLNNINVNLLAYSSLNTELDVIEIQPQKQLSELYYVDYKRILKLKMLDKLGR
                     KETAKVLYTFFEALPANPAPVSIERLRARLNLKSSVSVQNSVIRKAMKDLEAIEYLKF
                     SEIKNGRKIGFQIHKRNP"
     gene            complement(24578..24970)
                     /locus_tag="POB34_001495"
     CDS             complement(24578..24970)
                     /locus_tag="POB34_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918474.1"
                     /translation="MSDKVTVKQTINKATSIYKIEHITVGKPGSEQYRHAFELADQLG
                     LKHPDCIEHVFPTYADEQCTHVLTEEDFFSTEEREGVDRCIGVICSSVSYELFPNVHE
                     NGGIGYQFLYEGDDLKCYEHGLLIESVE"
     gene            complement(25148..25570)
                     /locus_tag="POB34_001496"
     CDS             complement(25148..25570)
                     /locus_tag="POB34_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ppfA"
                     /protein_id="HDI5918475.1"
                     /translation="MSFFSTLKTALSLKEKLAATGVLVLICALVGAGFAWERHQLKQA
                     MEKIGSLDQAVKERDKSIMDLNQTIETMNKAEQHFHSQEVKNESEQAKYADRQMERKA
                     EVQKQLVAAGNVRQRIPADTQRLLRESISEFNADADKG"
     gene            complement(25610..26398)
                     /locus_tag="POB34_001497"
     CDS             complement(25610..26398)
                     /locus_tag="POB34_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918476.1"
                     /translation="MLEKDYQLSAYKKLAAAGGMKTPGAITSARNSANTAKLLAEELT
                     GLILDTIVYPDTITSYVSTIRTTATGLTNIGGLATQHADLLAGYADLSMLLQLDIGWD
                     VYCRANEREVSELPISIAIGDVTITKSLEDAVNALNTSSLVAAMGEINQTLNTGSGSS
                     SGSGSGGGTATPPPALTEEQIESLKVATEQFGVVFNQTTAPTTALQQQYERANESANV
                     AITAYNHAIGTALAEASANKASTASAIAALVPDSVLDELNKAAQ"
     gene            26861..27145
                     /locus_tag="POB34_001498"
     CDS             26861..27145
                     /locus_tag="POB34_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918477.1"
                     /translation="MQMELISRKEFDSRVTSGELDNLQAIKVKEGFCLIGNQSGTNRV
                     FMLRRTDLKPFVWKNEIGPSSYAQTRGCHNLAFFYKDELSVVDIQGLQHV"
     gene            27138..28043
                     /gene="rdgC"
                     /locus_tag="POB34_001499"
     CDS             27138..28043
                     /gene="rdgC"
                     /locus_tag="POB34_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDI5918478.1"
                     /translation="MFKHWKNITIYKLSREADLTDLEDKKKMILFTPCGSQDMAKFGF
                     VSPFGDNSEVIAMHGNGFILVEAKRETKILPPPVIQRAIQEKIEKLEQEQARKLKKTE
                     KDSLKDEVLHSLLPRAFSKFSVIQAIYDGSTKRIYINASARQAEDMLALMRKSLGSLP
                     VVPLSVENPIELTLTDWVRDGSAPQGFQMGDAAELKAVLEDGGIARVKKQDLGSDEIS
                     THLEAGKLVTKLALDWQNRIKFTLDHNFSLTSVKFADELLEQNSDIDSEDVAQRLDAD
                     FFLLTSEISCLVDALVNALGGEAKQ"
     gene            28040..30463
                     /locus_tag="POB34_001500"
     CDS             28040..30463
                     /locus_tag="POB34_001500"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HDI5918479.1"
                     /translation="MKELCYGSVCSGIEAASIAWEPLGMRPAWFAEIESFPSAVLAHR
                     WPHVANLGDMTKLAQKVQAGEIEAPDVLVGGTPCQAFSIAGLRGGLDDERGALTLKYV
                     ELANAIDDKRSESFLKPTVIVWENVPGVLSSADNAFGCFLAGLAGEDAPFEPGDRPES
                     RKSNTFWRWDVKTGCHAPKWPQCGCIYGPQRKVAWRILDAQYFGVAQRRRRVFVVASA
                     RTDLDPATVLFEFEGVRRDIAPSRGEGKEVAGNVGNGIKSGSHWDNPVNPHPTLNQSH
                     NTGGIGASNQEIFAQRGSGLVYCMAHGQGGAEIKNDGTSPTLTCNHEAPIVFSCKKAI
                     AFTSKNYGQDAGVEISPTLRAGNSANSNQNAGCPPAVAYSLQHAQIGRKDDAGPQGKG
                     WQKEISFTLDSRATADAVALSFWGQESIEKGELGENGYLPHMMSVRRLTPVECERLQG
                     FPDGHTLIPTGKRKKVTSDELIYLRNHDPDLSEEEAAMLAADGPRYKAIGNSMAIPVM
                     RWIGERITKAACRQKEGSETKERKVKPAAEFERSIFKWAGGKFGVLEQIFRYLPEGKR
                     LIEPFVGGGAVFMNAGYQENLLNDVNADLINFYKTLQREAHSLITLAHRFFQDYNTQE
                     GYLAVRNAFNKQVYDDLHRAAAFLFLNRHCFNGLTRYNQAGEFNVGYGKYKTPYFPLQ
                     EMEAFLGAEGRSEFVCGDFAAVIEAAGEGDVIFCDPPYEPLPNTEGFTNYSGHDFKFE
                     EQKRLVSLLTDAHRRGAKVLITNSGAPNIRELYQDSGFRVEPLFARRSVSCKGDTRGV
                     AHDVIAILL"
     gene            complement(30522..30782)
                     /locus_tag="POB34_001501"
     CDS             complement(30522..30782)
                     /locus_tag="POB34_001501"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918480.1"
                     /translation="MASGFLGRYHHQIIVCIYFNGQFLNRRKVANHDENPEKNAHPKM
                     GKLEARNALRCCHLGVWSTWQLGVMRGKESLPRHTCRRACRG"
     gene            31139..31214
                     /locus_tag="POB34_001502"
     tRNA            31139..31214
                     /locus_tag="POB34_001502"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31172..31174,aa:Asn,seq:gtt)
     gene            31217..31292
                     /locus_tag="POB34_001503"
     tRNA            31217..31292
                     /locus_tag="POB34_001503"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31250..31252,aa:Thr,seq:tgt)
     gene            32220..32645
                     /locus_tag="POB34_001504"
     CDS             32220..32645
                     /locus_tag="POB34_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite/colicin resistance protein"
                     /protein_id="HDI5918481.1"
                     /translation="MLGILKKKFRKAAGGVKKMENRDAVEATVWGAYSIAYSDGTCDA
                     KEIAVLEKTIAALPAFAPFSGEIAQMSANIRAQYEASPRRANAQALRELADVSGTNDA
                     VDVLCLCIDIADQDGIGAEEQEQLKKIAQALQLPLEQYI"
     gene            32645..32809
                     /locus_tag="POB34_001505"
     CDS             32645..32809
                     /locus_tag="POB34_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HDI5918482.1"
                     /translation="MVIKARLILALVFLVLSVLVDFTSTILSVLSDGALVAVAVTLVW
                     PIFKPASKDQ"
     gene            32920..32995
                     /locus_tag="POB34_001506"
     tRNA            32920..32995
                     /locus_tag="POB34_001506"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32953..32955,aa:Ile,seq:cat)
     gene            33282..34646
                     /gene="dnaB"
                     /locus_tag="POB34_001507"
     CDS             33282..34646
                     /gene="dnaB"
                     /locus_tag="POB34_001507"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDI5918483.1"
                     /translation="MSASPLESMPNSLSAEQAVLGGLMLDNCRWDEVADRIVADDFYT
                     SAHREIFSEMERLLSHGKPIDLITLAEALEQNGKLERAGGFAYLAEMSKNTPSAANIC
                     AYADIVRERAVVREMISVANEIAEAGYAQDGRGSNELLDMAERRVFEIAEKRQKSGSG
                     PKDIASILDATVSRIEELFQRPHDGVTGLDTGFTDLNKKTAGLQASDLIIVAARPSMG
                     KTTFAMNLVENAAVRNDKPVLVFSLEMPSHQLMMRSLASLARVDQSRIRTGQLNDEDW
                     ARVSGAMGILLDKQNIFIDDSSALTPTELRSRARRVYKENGGLSMIMIDYLQLMRVPE
                     LQDNRTLEIAEISRSLKALAKELQVPVVALSQLNRSLEQRADKRPVNSDLRESGAIEQ
                     DADLIMFLYRDEVYHPDSEMKGIAEVIIGKQRNGPIGTVRLAFNGQYSRFDNYAGADW
                     QEDY"
     gene            34646..35644
                     /locus_tag="POB34_001508"
     CDS             34646..35644
                     /locus_tag="POB34_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918484.1"
                     /translation="MQWNEEKPMNILIIGRKFEAISDVKTYTEMWAYNLACAFSEAGV
                     TLQYHRPYSPGVESPEDYVEAVLTAATSCSAKAILAPGLRYFTTVPREIGVQLRRRFT
                     GWVAQVYDGSMLDSAPVDITFTVRDDTWRYLDNPGRLERHNRFNKHVGWAANQELFHL
                     ETKTDDVLRIFVDHAAFDVSGFDHSLSILMNLQRLTVPYEARTLTDDGLVTIDPGNIS
                     VTPYRRTPVPATEFAAELRKSDVFIVTHPESLGLTVLEAAMCGALVLTPPDCLPPDRL
                     ALVNHMVIKSRIDWDEVIARVDRVKNAEKVQCHTWSAIAEKMLETFITQKPSCGNG"
     gene            complement(35690..36322)
                     /locus_tag="POB34_001509"
     CDS             complement(35690..36322)
                     /locus_tag="POB34_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HDI5918485.1"
                     /translation="MGSLTGKYRAVVVSVDDPKGLMRTQIRVVGMMDGLPDASLPWAE
                     AILSNANTFSPFLPGDKVWVEFPYNGDSRWPLIIGYAQDASGGAPNVPPEASGQGEGY
                     VPPEVEGAPAQPSTSAKKDFISSRNGLMEVRTAGGAWAVTHLKSGTTIGFNEAGELYA
                     ISQGPAFISSAGNLDIKSGADVALKAGGSMAIEASGNLSIKAAQVSVDKA"
BASE COUNT         9378 a         8809 c         8470 g         9691 t
ORIGIN      
        1 gacggggtga ttatcaggca tcactatcca gcagcaaatc atctgcgcgg gtgcgatcaa
       61 acgtaggcgt tgctttcaca atagtgccgc ccggcgttgc agtgatcgga gcgctaatcg
      121 acgtaactcc agtaagcgaa gttgtatccg aagtttcaaa ccagcagtac gctttttcgg
      181 tatcagaaat ctcgttcaaa gtgatcatgt cggcgtgttc atttacaaca accgacaaat
      241 agagcgtaag cccatcaaac actatatgca gtggcagtag aggctttacg aactgattaa
      301 actttctgag aatttcttct gtaattgcgg actgatctat cgtgccagta atccccattg
      361 tccgggccag gtcgtttatg ggaatactga tcatccctct ggaagtcaga aacatctcgc
      421 cgaatgtgcc gccggtagtc tccagtgtgc tttcaggtat tagaaccgtg ccatagggat
      481 gacgctcaag gtccaccggt gcatatatcg gatcccataa aacagaaata ccgttaaatt
      541 cgcggtaaat tgtctggttt atagggcgtt cagtcccctt aaagtgaatc tcatcaagac
      601 gctgttgtaa caacatcgga acggaagatg agttcgacgt tctgatagta aagaactggc
      661 caagttcatt tgtcctggtc tccagatcct ccttgctcat ggaaaaaata gacttccggt
      721 tggtaattcg ctccaaccat gggtcaacaa aggtatccat cattgactga accaaatcag
      781 ccaatgattt atagagcaat gacttttgct tagctgatgt aagccggtta ttaaaccagg
      841 aacgctgcat cactcctcct catacgaaat attaaaggtg gagttttctg tatccagata
      901 aacgaaatcg taaaagccgt tggactcatt ccactcgaca aattccagat aaaagtcgcg
      961 gaaataaccc agcgtttcga taaaagccca aacgtctttt ttcttgatta ggatgtactt
     1021 gccgacgcgg ttcggatcaa agaaagttga gtcgcgccca aattttgttt ccagtgccga
     1081 cttcagctca tcggttacat tctcaatggt caggcttgcc gatatccgtc cggtgatagt
     1141 tatcttaaat ggtagttttc tgacctcttt atacgagaat ttcttgttca gttcattcgg
     1201 taccttctta aaggcagcta ggatcatttc ttcaagctct gactggcttt tattgggatg
     1261 ccatcctgaa ataaatatct tattgatatt ctgaacatta taagcaccat ctaatttctc
     1321 ttgctggcct tcgccccatg cctttaccca ggacagcccc gggatgttac gaaccagaaa
     1381 atacgtatag tccccgcccc atacgacctg atcatcatag gcaaggtaat attgtgcacg
     1441 gttacgtgtg atctccgttg tttcagcatc agtacctgcg gttataggtg tagttgtctt
     1501 aactgaaatc aaattagcta aattagtcgc agaatcgaca ggcgtcaggt tttggccagc
     1561 aaccagggtt atatcgccgc tggtgcacca taccttaagc gtaattgtcg agccttctgg
     1621 cggtatttgc ccaattagcc catcgccgaa tcgaaccccc aactgctcgg atggtttata
     1681 aaactcaacg tagacctggc ttttactacc ggctaaccgg aacatagtgc tggaagacca
     1741 ctgcgtggtc ttaccatcgg tcgtcacgaa tacttccagc ttatagcaga cagcagtgag
     1801 agcctttgat aacacgactt ccagaaattc tttggctgcc gtaacggtat atgtcacctc
     1861 ctggatttcc aactgtgcca cttctaccgt accggtgccg tcaaccaacc tgcatacatc
     1921 catagtcatg taagggtact ggtcgtcaga tattaaaggc atgtttttgg ggattaccgc
     1981 tggggcatct tcacttgtgg cagtgatctc aatcatcccc gatgacggtg ttggcttggt
     2041 accaacgtaa ctattcgttt ctgccgctgc caggatagag gaacgccgcg tcgcggtcga
     2101 tataaagcct tcagccagcg ccgcatcggc atactgaaag cacctgtaga caatctgggt
     2161 aataaacaat gtcagcatcg agacaaattg agagccgaca aacttcgacc agaatgaatc
     2221 tttctcgaca agctcttcaa actctgcacg aatactgtct ttagtcggtg ttgttttact
     2281 catagcacca cgtcctgtgt gatagttata tccctgatac gaatggatat tttcaactta
     2341 tcaaaagcat ctccctcggc aactgacaag ccagaaatcg gtatgtcagg taaatctacc
     2401 gtcagttttt gcaacagcat tgcctcaacc gcaatttgaa catgcgacaa gttggtcggt
     2461 tcgtgtttaa actgcggtaa aacattgccc catgacggat ccccgtatac ctcaccctga
     2521 taagtgttta gccactcata taaacgagcg ccccaggcct cctcctggga ctcatacgtt
     2581 tttacgccgg ataactccag cgtcagcaaa ggatcaattt cgttattgtt ggccatcaat
     2641 caactctcgc gtagtcattc atcaacggat catcaattga cagtggtacc gtgcgcataa
     2701 cgcccggctg aggcgtgctg acctttacga cagttccctg gcctttcgcc gagtctttgg
     2761 tgtgctcttc aatcctggca agcaatgagg tcatctgcgc aaacagccgc ttcgtttcac
     2821 catcaagtga aacggtatta tcagccaact gcattgtcgg cttggcacca gaaccgccaa
     2881 ggtcactaat aacctgcccg tctatctgca tacgaccggt tggttgctgc aaatcgttgg
     2941 tggcagtcgt cacttgggac gtggaggctg gttcaggcgc attatttttc cgcatccccg
     3001 gcgaattgcg gagtttatcg aatagtccat caatccccat ttgtgcgccg agttggtcaa
     3061 agtaacttga gttgctggcc accggacgcg cctcttcaac tggcatcgga gtatcaacat
     3121 acacattgcc agctgctgtt gcggtcccct tccctcgtgc acgttcttcg agcgttccct
     3181 gaacgacttc ccgacgcata ccccggccat tcatgaattt gttgaccaga tcgttaacgc
     3241 caacagcatt acctattttg tctaccagac cgcctttctc aaacgggcta tcaccagggg
     3301 taaacgccag gccagtagac tgatcgataa cagcgttatc aggcagtggt cccctcactc
     3361 catattgcgc cccaccctgt gctcctgctc caggtgtata gatgtcacca cctaaatagc
     3421 gagcacgatg agtattgacc ttgatcgcgt actcacggtt ttctttcgat aagtcaccgg
     3481 tgcctttttt ccacttatta atagtgccaa acccagcgtt atatgcagtt atggcctcgt
     3541 ttaaatctcc attggcttgc ttcagatact tgctcatgag aagagccgca gcttctgccg
     3601 atttcacagg atcaaatgca tccggttccg atatcccagt ctctttacga gcaatactcg
     3661 tgaactggaa catcccgagt gcgccaccac ttaactttcc atctggtgat cttgtaagcg
     3721 gagactttgc gttaggatcc cctcctgatt cagttgcagc aatcgagtaa agagtgcctt
     3781 ctggaaggcc atatttattc tctagttcag caaaatacgg tgccaactta tctagatttg
     3841 ccttaccttc agcaccaaga tttccgactt ttacatccaa gttgccattg ttgtaggtat
     3901 ccgctgcttt ctgaatgtca ttcctggtgc cagtggtatt aagcgacgat gatgacgagc
     3961 tattttgtcc aatagcatta tcaattttct gcaacgcgct attgcccgtt tctacggcat
     4021 ttgcattgat aatctgatta gcagtttctt taactgtttt attgctatct ttcgccgtgt
     4081 ccagtgccgc atttatcacg cgggtaacaa tattagtctg tttggcatcg gattcagttt
     4141 tagaatcaga tgtctcctgg tggctattaa ccggagcttt taactctgga gtgatttctt
     4201 tcgcattagc ctcgccgatc ggattgggta tttttgatac aatcattgcc gcaggggtat
     4261 ttttaacggc atcaaccgct gcatctaatg ctttaccagg taaattttta accccatccc
     4321 aaatattacc agccgcctct ttaatgtgtt tccctgggtt cttaatgaaa tcaattgcac
     4381 tatcaattgc atcactgaaa acctgtttca ggttatcaac agtaaagaag tctttgatgg
     4441 catccagctt ttcaagcagc ttattagatg tatcgctaaa ccatgctgaa acagcatcac
     4501 caatctttgc tgtgtaatct tcgaacttgg tagaaatggt gtcgccaagg ttagaaatat
     4561 atgtttctaa gttggtaatc ccactatcaa tggcctgggc aatactttcc gtcgaaaatg
     4621 attgcaacat attgccgata tcctcaaatc caagtgattt gagaacctca ccaatggcgc
     4681 tgctaatacc agataccagt ccccccatat caagaacatt ggctaacgta taagcggctt
     4741 tttgctggaa tgatggatct tgtcctgatt taagcccaaa cgctcgacgt tgcgcttctg
     4801 tatcattcca accggttacc gcatcataaa tacctccagc cactgtgccg actaggggaa
     4861 ttgcgcgtaa cgccccttta ccaactgcct ttaatccaag tttacctgct gcccgggcag
     4921 ccaaatctcc accttcatgg gcgagagtct tcttgccacc accgcgtagc attcctacaa
     4981 gtttctttgc ccccagagcg ccaaaagcga gtgctccagc ttttttcagc atgccatgcc
     5041 ccattaacaa cgacgcgacg ccaccggccc ccttccctaa caggctaaat agtttggaca
     5101 gcaagccgcc cttctttttc ccggtgtttt tggctatctg atcaagggcg ctgagaatct
     5161 tgtcattgcc ctctttaatt tcgctggtct gctcctgaag ttcctgaacc gtccgttttt
     5221 gggtgttaac ctgaacgaca tcggcactat tttgcgattt acgcctaaaa aaaccttttc
     5281 tacggctgtt atcgtcattg ccacgaatca catcggcaat agactttctg gcaccattaa
     5341 gcgatccacc aacttctttt gatatcccgc caagctcctt ccctgctgcc cacaatggac
     5401 cagcaacggc ataacctaac gcatcgacgg cacgagtctc tgaagagtta cctatgcctt
     5461 cagctacttt tgacagtttt tttaataaac ctgattcaac atttagacgc tcatcatcct
     5521 ctttgcgcct ggccttttca gcacgttcag cacgggcatc ttccgctgcg gccttactcc
     5581 ctgactttcc aataaaacga ccacgcgcat cgcgttggtt ttggcttttt tgcgcaccgc
     5641 ctttttgatc gaacatttcg cgagcgtgtt cggctgcttc ggtccgttgt gcctttatat
     5701 cctctggtat agccttcctg cgtcgttttt taccctttgg tgtagttgat ttgtcctgct
     5761 gttcctgttg agcaatgccc tcctgaacta cacgagaaac gtcccctaaa ttaagccgtt
     5821 tcattgcgtc aacaataggg tccactgatg gcgcattggt cacaaagtca ggacgtgaat
     5881 tttcgattgt gcgatttaat gccgacacac tgcgagagac aggatcaaca gttgcaacgc
     5941 gtcccccttt caaatcttca acagcttccc ggatacctgc aagctcttcc agctcttttg
     6001 cgctggcggt ttcaactgtc cttataacat cgtcaatgtt ggcgtttttt ctttccatga
     6061 tcttatcgcc taccgtttcg gtttaagttt ttcttccagt ttctccaaca ggaaaaacgc
     6121 ataggattca gtaagccttt cagcgtcctg aatcggtata cccccataca aaaccaggtt
     6181 ggacactaag gtctgatagc ttttcaatcc ccacctgtgg aatgaagtcg gtagcccgaa
     6241 agggcaccca cagacgggta tacgcaccct ctgtggactc ctttttatcc tgatttgggc
     6301 atttatgcgg cgggagacga agacgcattt cacctttatc gatgtagcac ggtaaaccat
     6361 gttcgagctt ttcatgagcc agtcggatgt gtgccgccag cttcataaat tcagtatcaa
     6421 tggccatccg tttaatcgtt tcataacgac gctcagcctg atcttcacga gtaccgctaa
     6481 catcgttata aagctcacac tgataagcga attcccaaaa acgcaaatca acgatcgctt
     6541 ctttgaattc cgcgtcgtct tcaggtggca atgctgcacg gcgcatctcc agcatttcca
     6601 ttgcccaacc atcaagcggc acgatacgcc attgataagg cacgccctct acagacacct
     6661 caatatcgtc aatgaacggt tccacttcca ggacctggat atcttcagcc agagcattca
     6721 tatcgcaatc gtaataatgc tctttaccgc aatgtttaca ggtgtaggtg aatgtctcga
     6781 ccggtgtttc acgggagcca gtaaatatcc accataacgc ggtaatccgg tcctgcgccg
     6841 tccatgtcag gggatcatat ttcgcgggtt cagccagcaa ggcttttaaa tacgccgttg
     6901 tctgttgttc ttgttcctcc ggtgttatcg agttgaaacg catcgcatca gcaatatttg
     6961 gctgacggaa ctggatcaat tcagttggcc gcgatggtag cgggaaaaga ggtaaaagca
     7021 tccttgctcc ttaattcaaa gagaaaaact aaagcccaga agggaagcta aagaacttga
     7081 ggattggtta aacgtgctgt gcaatgcgaa ggtcattggg aatgacttaa attccgtaac
     7141 ctgatcccgc gcataggtga catcgccggt agtgaccggg aataccgtca tctcattttc
     7201 cagtttggtt aaaccggagg acagcaaccg ataaatacgc acattgagca aatatttaga
     7261 cggtatattc ccggtaccgt ccggattgat cacccgacct tttgccgtct taaaccagtc
     7321 caaaacgagg ccatcaacgg tatccctgac catcattgtt atctgcccag gcgaacgctc
     7381 cgttggctga aggatattcc ctccgccgat tttaatcgtt tcatattcga tgctgtaatc
     7441 gtggtaggta atgtctttag caaagaagtc tgccccctcc agaccatcaa cttcgacaga
     7501 gaactgccat ccttgcgcga acagcatttt gttcatgatg attgacgtca gcttaccaac
     7561 ttcccgctca ccaacgccgg agccaaataa tgtcgtcgtt aatgccgaag atacataaga
     7621 ctttactgaa gcaacattaa gccccatatc agccccctca cttcaacatg gatgagaaaa
     7681 gaacaattcc cgggataatt gcccttgttg cgctcatttt ctcttccaga tccagctttc
     7741 gctgatacag cgtgttctcg tcggataaat tgctggcatc gagtttcccc gcgatagata
     7801 ttcttcgcag gcgatcagtg ttaggtattg cgattagcac ttccagatag tcagaaagta
     7861 acccaattat ttcaggtggc acttccccat tatccagatc catatcacgc aaattagcca
     7921 gaaatgacac attcagtggg tataccgctc gatgggtatc ttcaagctcg atattcccat
     7981 cgtaaacgtc ggagtagaca agatcgccgg tatgatccgt aaccgatacg agcgcaagaa
     8041 aatcagcagg gcaagcaagt gatttacagg tctgatcagt gaagcgtatc cgcttgatgt
     8101 gtcccgccct atcctggtag gttcccaatg cttttcttag tagggattcc agtaaggcag
     8161 gttcatccgc aatcaaaggt gtgaagcggg atttgacgtc ttcgagtaat tgtcgtggtg
     8221 tcattgaaac ctcgtagaat ctagtttgtt aacagattct acgagttgtc atttgtgaca
     8281 gtatgctcgc aatcgtagtg attactacca ccaactgcta gctttcttct ctctagcttt
     8341 tccttcgctt tgcagtaaat ctttacttcc tgtagaaata tttctatttg catcagcctc
     8401 agactgaatt acatctgtct tagctgctct ctccttcaac tcctgctcta taaaatcgtt
     8461 ttctcgctgg acacgagccg cttttgcttg cagttcgagt ttcatgcttt cgagttgcag
     8521 cttgcgaagc tcatcctcat aagcttgatc tcgcttcttg tcatttgcta gctcggcttc
     8581 tcgtttcgct ctacgttcat tttctgcagc aacacgctgt ttttcgcgtg cagcagcggc
     8641 agatgctgct ttttctcttc taatacgttc agcttgtgct tgttggcgaa taagttcttt
     8701 tctggcatcc tcagcttgct gctctttagc tcgcccttca ttttccgctt gagcaatagc
     8761 agcaatttga ctttgcaacg agttagccaa ggcagtgcct acactaaacg tagaaaagat
     8821 tagaaataaa ataattttat tcataaatta tttcttttct ggacaagtag catttggctg
     8881 aattcgagtt tcattatctt tagttgaaat aagcacagca agccccgttg taaactggca
     8941 tgctttacca acttgagttg aagtgaacac cttagttcct tctttataag tcagtgaaac
     9001 accttcaaca attgttttat cactaaccaa agaaccagcc gctgcaccaa gagccgcacc
     9061 accagtaccg ccagctacag cacccagtgt cgatgtgcct ttaacattat aaccggcaac
     9121 gcccccagca acagctccca gtaccgcacc aaatgtctgt gccgcttctt tattagcttt
     9181 attatcaact ttcactttag ctggaagcac agagataata ttgactgtct ttgtttcctg
     9241 cttcgaattc aattgagcgg tatcataaac atcggcagca taatcagcac cgctagattg
     9301 acacccagaa agaagaattg cagatacaac aagagcgcat aaagtatact ttttagacat
     9361 aagaatcatc ccagaaaaac aaaaaccggc gagaagttac cacgagaacg gaaagtaaac
     9421 aagaatactc ccattgactt atatcaatta atcaactttt tataagctat ccctaactta
     9481 ttgtgtatag ctgaaaagtc agagacaagt tcaccatctg cattatttct taagttaata
     9541 gtttattgct aacttaattt atgtatcgac tattgatatc catcttaaag ttacaatatc
     9601 cctctgcatt atttttcatc acaaataata tatctattgc acgacgctaa tctgctctaa
     9661 taaatttgta tttttaagtc gcgaatgcta tctttttgca tcatattgac cttttaatcg
     9721 ttcaggctta tagtttcgcc gtcgtagcaa attctgcgac cgggtttagc agcctgaatg
     9781 attgtgcgga cagccgcaga tatccgatat tgcggtattt ttgtgtccgt aaaaccgcgt
     9841 tacgcccaaa ttatggtggg gcgtgatggg gaggcttcgg cctgctggtt tcacaatcgc
     9901 cagtctgcta accccgtcac gtcctgccac ctgtttagca gcgggtagca ggttgttaaa
     9961 cctgattgtg aggccgtaac tatggttaat gccaatcctt gcgcacgcca agaattcatc
    10021 tggcgtttct attcctgcaa aaaacaccac tatcacttcg tcatcgcagc aacagaagac
    10081 gaagcacgct ctcaattgcc tgatggcccc tgcattttta ctgcccgttt ttcaactaac
    10141 tcgcgcaatt cacttagtta ctggaacctc cccttctctt ccgacgttca gggggtttta
    10201 tgaaaaaacc tctcgtcacc cgtaatgaca tagccgaagc gatcgccctg catactgcct
    10261 gtatgccgac acgggagatc cccggcgcaa ttgccaacta tttcatgata accagacgtt
    10321 tttatacccg aacagataag gctgtgatca acaggctact gatagccgag atcagggatt
    10381 atttgattga acaaggacgt ctacgttacg caacggtggc agcagaaatg agaaaggagg
    10441 cacatagaat gaccagtaat aatttgaatg ttaaaaaacc agcatctttt gcttcagcta
    10501 cgccatcacc agctgtgaat gttatctcca acactggaga cacaatcgac agccagacgc
    10561 tgttaaagat ggtcaatgaa gcgcgtaagt tatgtgggga accagaggtt cggaacaaca
    10621 aattcatcaa aaaaatactc gacgaattag aaggtgagga cggttacaca aaaagtgcaa
    10681 ccgtgccgcc aggtggcggt acgcctatgg ttgtcataac catgacctac aaacaagccc
    10741 tgcgagtcgc cgcgcgcgag tcaaaagcag tccgccgttc gctgatcgac aaactggaag
    10801 aattgcagca ggcaaactcc cctgccccat cgatccccca aacattacca gacgctctac
    10861 gcctggctgc cgagttggca gaacagaaaa tgcagttgga acaacagctg gtggccgcag
    10921 cccctaaagt cgattttgcc gaccgggtat cagcggctaa tggaatcctg atcgggaact
    10981 ttgcaaaggt cgttggactt aagcaaaacg cccttttctc atggttgcgc cagaacggca
    11041 ttctcatggc ttttggtgcg cgcaaaaacg taccgcgcca gcagtacatt aacgccgggt
    11101 atttcacggt gaaagaagtg gtgctggatg atgaaaatgg ctaccagata cggctgacgc
    11161 cccaattaac gggtaaaggc cagcagtggt taactcgcaa gctacttgat gctggtttgt
    11221 taaaaccagt agcaataggt taacaaaaga aaaaacctgc cagcaaactg gcaggtttct
    11281 gagcagatcg accaacccga tctggatcga gtcagaaaaa tttgctctaa taaatttcgt
    11341 tttctaagtg caaagaatca ccatttcgag ctggtgattg aaggttgatg caaatttgga
    11401 gaaaaaatgc aacaaacatt caatgcggat atgaatatat caaaccttca tcaaaatgtc
    11461 gatccttcaa ccactctgcc tgttatttgt ggtgttgaaa ttacgaccga ccgcgctggc
    11521 cgttacaacc ttaatgctct acacagagcg agcggactcg gtgcccataa agcgccagct
    11581 caatggctaa gaacgctgtc agccaaacag ctcatcgaag agcttgaaaa agaaactatg
    11641 cagaattgca tagtttcgtt cgaaggccgt ggcggcggta cgtttgccca tgaattgctc
    11701 gctgtggagt acgcaggctg gatttctccc gcgtttcggc tgaaggtaaa ccagacattt
    11761 atcgactatc gagccggaag attgcaacct gctattccgc agagtctccc tgaagctctc
    11821 cgtttggctg ccgacctggc agagcaaaag caacggctgg agcaaaaaat gcttatggat
    11881 gcgcctaaag tcgaattcgc cgaacgcgtt gctaccgcca gcggggttct aatcggcaac
    11941 tatgccaaag tgctcggcct gggccaaaac tatctcttca cctggttgcg tgataacgga
    12001 attctgatcg caaccggtga acgcaggaac gtccccaaac aagaatacat atcccgtggg
    12061 tacttcaccc tcaaagaaac cgtgatcgat acaagcaacg gaagcaggat ttctttcacg
    12121 actcgtataa ccggcaaagg tcagcagtgg ctaatgaagc gattgcttga tgctggtgtg
    12181 ctggtacatg tcgcggctac gcgctaacag acgtagtaag aaccaccagc attgtaatgc
    12241 tggctaaagt cactttcctg agctgtataa cgatgagcga ttttactttt tctggctatg
    12301 aattggcctg ctttgtaaca cactccggtc tatcccgtag caccgggcat atcctgtcgc
    12361 aatgtgcaaa tctcgcggca acaaccagtg aatacttcat tcacaagcct caccgcctga
    12421 tcgcggcaga aactggttat agccaatcaa ccgtcgttcg tgcattccgt gaagctgtaa
    12481 acaaaggaat tctgtctgta gagattgtta tcggcgatca ccgtgaacgt cgcgctaacc
    12541 tgtaccggtt tacgccatcc tttttggcct tcgcacaaca agccaaaaat gcgctgattg
    12601 aaagcaaatt aaagatctct tcagcggcaa ccaaggttaa agctgttctc gctaagacat
    12661 tggctttatt taatttttta tccacacccc catgtcaaaa tgataccccc tccccctgtc
    12721 aggatgacgt ggcaataaag aataagaagt cacaagttaa aaaaacaaaa agatcagttt
    12781 ccggcggtgc cggaacgagc agactcaaaa aattgacttc atggatcgct gaggcaaaag
    12841 caaaggctga caatctgcgg ttatccaaaa agcgcgctca aaaacatgag ttcaagcaga
    12901 aagtagaggc ggcagcgcgg aaatatgctt acctgaagaa caagcgttct cctgatattg
    12961 gcggggtatc aaacttcgat aatttgccgc attgcatgac ggtaaacgaa gctcttaatg
    13021 cggttttagc caaaaataaa gataacgaac aatggggtat accggcagga ttcagagggt
    13081 gatagattgc tctaatctgg agtcacctgg cgttttcagt ttgaggtcgg agatgcaatc
    13141 tgatttttta cagttagcga tcgcttttgc aggatatgtt tgtattggct tctgtgtata
    13201 catgatcagc cgaaaaatgc ttgtcgatat cgaccgcaaa gaacaagcag aggagatctt
    13261 agaatggatt ttctttggcg cggtctggcc attagggatc atgtttgctg caacatttct
    13321 tctgatgtgg atattcaccc ttccaggtga tttctataga aaaaaagcca gacattgata
    13381 caatcgttgc gggtgcttga ggctatctgc ttcaggcatt acccgaaaag cagatagaag
    13441 aaagccccag ataacattac gcgtcctgca agacgcttaa cattaatctg aggccatatc
    13501 tatgcttagc atacgtagat taaccctctc cccttttaag gagcaaggaa ttttttactg
    13561 ctatgccgca tttccaatta tggattcagc gaccagaaca accccatgct tacttcgatc
    13621 tgggtgttca ctccatatct cggattcagg aatacttacc ccgtcatagc aaacacgttc
    13681 gccgtcaaac gggtaatact gagcggcatc ggtgtcaagg ataaacactt ttagctcgga
    13741 agagcgacgg gcgatgtgtt gccaaagagc ttttccacct tcgtattgct cattatcact
    13801 gagcaaaaca acaccagctt ttgaggctac ggtttcgtaa acaaaagtag ctaaaccgac
    13861 atcccggacg gttccatcaa ccacgatacc ttcaatctgg gcaacatcgc tatcctccca
    13921 gaacttcctt accagtacgc cttcaagctg cgggcgacgt tgaagttcaa gagtagcgac
    13981 aaccttatgg cgcatatcac catcgataat agaaggcgca gcctctatta cagctgcaaa
    14041 tggggttttg gaatcccata taacctggta aagcacacct tggatggtaa ctccgtccag
    14101 gatcgaaaat cgacgagcga cagtacccgg ggagtacgac attggaatca cagccatttt
    14161 agagtccccc tttaacagta caggcacaaa cagctacctc aataattgta gtttattaga
    14221 acattaataa tctaacataa aactccagac aatgttatcg atgcatgttt atataaaata
    14281 gttataaaaa aagctcccga aggagcttta aaatacaagg gatgactctt aatcccactc
    14341 aatccagttg tagacgatac gaagtgacgg gcgcacagcg gcagtcacat cttcggtact
    14401 aaagtcgatt gcatcactgt agattttgca gtccaacatt tcaattgttg tagcagcttt
    14461 tgtcacagcg ttaaccccgg aagatttgga ttcaggggtc gcagccatcg tgatatcaac
    14521 atagtccttc gccgcaatgc gatctttaat gaactgaaga atatcgcctt cgatagtctc
    14581 cacgcactgg acctggattt ccccggagtt acggattgga ccgtgctggt tgaacttcac
    14641 accatttggg ccatagtcct ccacatcctc gcgggtcatt tcagggattt gcgacgtgcg
    14701 aaccagtacg ctgatatctt catagcctgc aaaagtgagc tggaattcag aagataccag
    14761 tcgttcgcct ttggccgcgt tggcagtata gcggccctta ataaatttac ggtttccctt
    14821 agtgttattg tgccccatat aaaatccttt tactggaacg cccgaacaat atcggagctg
    14881 ttatatatcg aagaaccggt caactggagg ttgacggtgt ttttcaggaa atatccatta
    14941 ctgtcccttg gcgcatcgag atcaaaactt aagtcctgga tggcgacatc ggtgatgttg
    15001 atccggcgac caatgtttag cgtcacgcgc tccgggattc gaccaccaac attggccgct
    15061 tttagttccg ggctaatcat ggctgtcaac gcggcgatag cgcctgaaac ttcaatgaat
    15121 ggattgttca aagcaatgaa agttacgggc agcgtgaacg tcggcggagt ccccccttcc
    15181 caaaccatta agctattcca acgggcaaca gacgttgttt cagtacctac ttgcacaaaa
    15241 ccacccagag caccagaaac agatcccatg gacataccgg taaatggcgc ttcccaattc
    15301 tgggccatgt tcattgccgc tccctggctg atatatccgg taacctggta ctgagagttc
    15361 gttaaagtaa ctttcagata tggcgataca ccgtcagcct ggctgtaaac cccataagct
    15421 ataggagcca ttcaagttaa aggccggagt tctccggccc cctcctttag ccaaggcgct
    15481 tacggcgcag tttcattgac tttttgcggg caagttttgc cgcgccggtc tgggcttttc
    15541 gacgcgcttt tttcagcgcc gatttttgag ccgcagtcag acgtttttta cgcaggcgtt
    15601 tacggatgag tttgatctca ccgttacgaa caaccttctt aaatgcttca gtcagcattt
    15661 catcagaagt gccagcaaca acaaacgccg cttccagttc gtcacggtcg tcgctatcta
    15721 aaccagcgat agaggcacca acatcagcag cagcgtcgtc gtcttcatcg tcagccagtg
    15781 cttcgatcag gtcatcatct acaccgcatg ctgcgaggaa gtcagcaaca tttgcccatg
    15841 cttcgttata ggcatcgtcc tgttcttctg taacttcgga gtcgtcgtca tcagagatac
    15901 cagcgatagc ctgaacgaaa ccatcaaggg agtcgaaagt cagatcaccg ctatcagccc
    15961 aggagaaaac ggcgtcggcc gcatcactca acgcattctg catagcactt cgatttgcag
    16021 cttccagaat catctggtgc gcctgttcga cggtccattc tttaccgtct ttcccttcca
    16081 ggatttgctc tggagccggg gcagatggaa cgttatcgtt agtctgtgcc gccggttccg
    16141 gattattatt aataaccgga tctgttggcg gttcggcgct tgctcgggca gactccatca
    16201 gctgcacagg atcagagttc aaagcgaaac gggacagtcc attccccaaa aatgccccgg
    16261 attgaaaaaa gtttttgctc attgtattcc cttacttaat aagcagcggt acgccctgga
    16321 tacgacgggc tacgccagtc gggcagcagg cccagactac ttcccattta tcgaattccg
    16381 cctgcgtaac tttcagcaca tacggttctg taccgtcagc atcaggatca cgaggagcca
    16441 ccagagcgcc ggaggcgaca aagcgatcta aaagtttggt catcccttta gtcaggccag
    16501 ccgcagtaat accgtccggg ctatgcttca tctgtcgggc taactggaca aagaaacggc
    16561 tgattgcatt catcagggat gggacgtgct ggaagtgcag atagttatcc tgcgtgcagc
    16621 aagttaaagc atcgtcgatg atcatctggc cagaggtgcc aacagatact ttattgagac
    16681 ggcccttgac cattgcttct tcgtccgggg tatcttccgg atacagcggt tgaattgacg
    16741 cacgagcaat gacggcacgt tcttcaccag ccggtgagta atgccaaccg ccgacatcag
    16801 agtttttctt gacgccacga gctttcgccg catacgccgc gccagacaga ccgaagacca
    16861 cacgggattg ggtccatttg tctttgcagg agaacgggaa gtgatagaca gcacagctta
    16921 cataatcggt accaagtaaa ccggtatctt caacagcaga gagcgcttcc gtatacgtca
    16981 atgtcggttt gacatcaaag aagccatcaa tcaggcgatc agaacagata ttacctaacg
    17041 cggtgatcgc cgcattgtca tagcaaccca ggccgagaac agcggtgtac atgtacggcg
    17101 cattattcag cactttaacc gcacgcaggt acgcagcggt tgagattttc gactgatcgc
    17161 cgttggtacc gccagtgaac gccaacgatt ttttgtttgt tactttcgct gtcgaaatca
    17221 gctcttcatt aacaaccgcg cgcagatatt tagaacgggc ttccagagcc gtaggcagat
    17281 aacacaagcg gcccatgtca tctttcgctt cttccgccaa agacacagtg tgtgtctcca
    17341 gggtcgttac cacgccgagc gaagtcgtct gggtcagttt taagaggaag cgttcattac
    17401 ccgcgctgtc cgctgttgcc gtttcgatgg ttaactcacg ggtaggtgaa atacacggat
    17461 caccatcatc aacgtagata gcaaaggctt cgccactatc aagttcaatt tcagaaccgt
    17521 atggcaacgc actgtaagcc ggttcgcctg attcatcgaa cataataatc gggaacttcg
    17581 catcatccgg aacagcgcga acaacataac cagacgtttg ctgaatagct tcgtatacat
    17641 ggcgaattgg ttcgaactgt gagccggaag acggcttcag cggttcgccg agaacatctt
    17701 cgtaattgga ctcagtaacc gcaagaacag taaacggctt gccacgcgca aatacgccaa
    17761 taccagccca taagctgcta tttaatgcaa caccggtaga taacgtcgca tcggcattga
    17821 tcgggctaac cgcgacgccg gatgcattac ctaatgactg ttgaattgaa tattgagaca
    17881 taactttccc tgttatgcgc cccgcacggg ggcgctatgt taaacggaga acttcccctg
    17941 attactcaga gtcaccggca tcaatcgtgt cgccgctaat gaagttaagc ccgccttttt
    18001 tggccattgt cagcgttaca cgagtgaagt aatcagcgcc gttgcgtggg tgcatatcgt
    18061 tgatagccga accccacagc gtggtacggt tgaccagcgc cggagtggtc ggatgctgga
    18121 acgggatggc cgggacagca tcaccagtca cgaagcctgc tttacccgga ttttcatcac
    18181 ggacgtagca cagcacatcc atcgagctga actgaatgtt ctctgtcgtt aagttcttac
    18241 aaataccagc aggtacttcg tacactttca cgttaccgaa cagggtaccg atgtagtgaa
    18301 catacggagt ctggatatag tcttcggctg gctggaagaa atccttcggc aactgtttga
    18361 agaaagatgc tgcatcagca ccagcaaaca tccccatcgc accagaagat ttaacgcgct
    18421 caataatgtc gcgatataca gtctggaatt tgccacgaat gatggttgcc catacatcaa
    18481 aggactggtt aaccggcaga gcgatgtcaa aggtgtcgtt cgcaagagta cgccagatca
    18541 tgatgcgaag acgcagcata tcctgttcat gagacaggta ttccttcagg gtgcggaact
    18601 gtagggaacc caggtccaga ccaaattcac gctgtgcttc atacgccgcc tgtaccgtgt
    18661 gctcagccgc gataacgaac tggcttggga acagggtgta tttcttcatt tcgtggttga
    18721 tcagcgggat cagctcagga gcggcttcaa tattgatttc cgtctcaatt gcgatctcag
    18781 tgcctttatc cggcgctttg gagaacgaca gggcaatctg accaatgttg tagttcagag
    18841 agcaggtaac agtgatttgc tcaccagcag cattagtaaa cgagtgaagt aggctgccgg
    18901 aaccgttatc aacaacagac ttaatacggt taacgtagat attagtgcga ccttttcgga
    18961 ttggtacatt ctggccttcg aagtcttcca tcttgaaggt tgcggttttg ctggtgccat
    19021 cggagcttgc caccagcaca tagcggcggc gtaactggct gtacacaccg acggattgca
    19081 tgtccagaac atcaccagca gcataagaac caaaagagga acctgccacg ttaaagactt
    19141 catagatgtc ggactggtca cgcgtaaccg gaatgaaggt acacgcatca gcggtagctg
    19201 cccccaactg aacaggcagg atcatcgcga ggaataaagg cagacgcata acaccgtcag
    19261 aaacgctcat catctctgct gcgacggatt ccagcatcgc tttattagtg gcatccatgc
    19321 tattgcgggt ggactcaatc aggcagtttt ccagcgtctg gtggcaggag gccagaattt
    19381 ccggacgcgg catagattta tgtgctgcgg cgtagtcagc cagtgcactt gcccacgctg
    19441 tagcgatttg agcggtggca ttatcagaga tacccgcaaa aaccgggtct ttacgtgcag
    19501 cttcaaggat agatgcggca cgcgcggcat catctttaat gaattggtta tcagtaccga
    19561 actgcgcagt gcttgcccag ccaagcacag ctttagagcg ttttgcgata tctgcaatac
    19621 gattctggta ttcgcgtaag ttactcaatt tactcttcct taaacacaag gcacttgtgt
    19681 gaatcccttt tcggaagaga ttttattgaa agtcacttgt tgactttctc gtgacaagca
    19741 atttttttat ttttttcggg agtaggggag gaaggtaaaa tccaaggtga aatcgtggcg
    19801 atttcacctt gaaattttag attgatttac tttaaaaaca gtaggttaac agtgaaattt
    19861 gaatggcgaa agtttaaggc ttcggctttt tatcgaggct ctttctaagg atatgcccaa
    19921 tcatcctgtc gagttcttcc tgtagctctt ttgaaagtcg attaaactca taagaaaatg
    19981 cacggccttt cacgcgcttc cttgcaaagc gatccttgtc ctcaaatttc cataattcag
    20041 taactacgga cttatcttta gaacctttat ccgtgagtag tgaggcttcc tttgttatca
    20101 agcgcaggat tttattttta acttcatctt cggccatttc ttcaatggat aagatgtcgt
    20161 ttatttccgg ggatatgttt tgaataagct gatcaaactc taaattcttg ttccccattt
    20221 cgtcgccaac agcacaaagc gttttgtagt ccgaaaaggt taattccgac tgcacaggga
    20281 aaagggcgac taattcttcc ggagcactcg ctgcctggag agcacgcgtg accttcgcct
    20341 gagacagtcc ttctttggct gcaatatcct tctgactcat cccatcattt ttcattcgca
    20401 tcaaacgcag acctatttct cgaatgctgt gctgcaatgc tgtctgaacg tctttcgcta
    20461 aattttgcgc ttcctgaacg ctgatctcct ggtccgtgac taaaacccgc aaccctacgt
    20521 tctctaagat ggcagaagct cgacgccggg aaccatccaa aatttcaatt ttccctgtag
    20581 cccgtctgac acctattgca gggtaaaatt gctgatgctt aatagtgctt cggatacttt
    20641 ttaatgattt tggcgtaaga gatgcctggt cacgcccgtt gttatgctga tcaacaaagg
    20701 tatcgctttc tacctggttc ggaggtatta cctcttcaat aaatgtggcc tggcgaccag
    20761 ttgataactt gaatacctgc tcgactcgat cgccagaggc tgaagaacta tcaaatccgc
    20821 ttaatattga aggattaagg gttcgcccaa ttgttggtct gtttttcttt gacatggggg
    20881 tttcttactc ctcagttaga tctgataaat tcaatacggt caaaaactgc tttagcaaaa
    20941 tcttccgcag caattcgcgc gttcttcaat gcatcagcac taccaacata cgttgccggg
    21001 ttagctgaaa taacagtgtc aaaagactcg ccgcagcgtt caaaaccgtc aaggcgaggg
    21061 aggacaacat caagcatatc cccaccgaac acttctttag ccaggctatg gcaatactta
    21121 tggtctgcct tgttactcaa cttggacata aatccaatgt tagttgcaag ctgacactca
    21181 cagccttcat ccgaaatgag tttcaccaac tcaggaaggc gggcaacgta tttaagcgat
    21241 gagtggaaat caaccgttgc aggcggcaga ggtgtaaaca gtatattggc cgaggccaaa
    21301 gcatttttca ggaaggcgtc aaggtgagga ccactatcaa cgaggataaa gtcataatcg
    21361 ctcttcagct tatcaatcac attttctttc aggactgcat ggatgttctg acccggtaga
    21421 tgctcattgc acagctctct ccaatcggat gcaataaagg catcgtcaat cgacgcaggc
    21481 ataacgtcaa ccccaggtac aacagaagga acaataaact cctctaacag ctcttcacgg
    21541 cttacattct gcaacatagc ctgtgcagat gttgcgttta cgataccaat agagtgttta
    21601 tggcttaaaa acatcgttgc tgaagattgc ggatcaaggt caataaccag aatccttaaa
    21661 tcttccatca gaagatgagg atgagcacgc attgcatgcg ccagagaaac cgtcgataca
    21721 gtttttgaca caccgccttt aagattggag atgaaaatca catacgcttc gctgtagcga
    21781 tcccggtatt ttggcactcc gcgatgttca tatatgtcaa tgatgttctg aattgacatc
    21841 gcatatttca ttgaagagcc agcagggcgt ttatcgaaaa cataaccctt ttcttccatt
    21901 tcacttacgg catagtcaac gttcgcacga gtcagtagag gcaattttgc cagtgccgct
    21961 ttcgcataga cctggtaaaa ctcgttcgcg tgtagctcat ccttttgcaa ctgtacttgt
    22021 tcagtcagaa cattgagcat tctatttgct ctttgagcaa ccttgtgaag ctggctggaa
    22081 tcactcatcg aaagtcatcc tttatgctgt atttttgaat ttaattaaaa atgctgcata
    22141 aaataataat gtatgcgtag atgcttgtac atagcattct ctgcatgttt ggttcatttt
    22201 gcacgattga gagttacaag gagggcacaa aaaagccccg ttcaggggca tcagtgttat
    22261 ttgctaagag cagcgaataa tcgttcgaaa tcgatagtat ctatagcacg cgtaagcgcc
    22321 ggaagttcag cctcaaagta cccgtgtcga tcgtaaaaga agggaccgaa gagcgaggca
    22381 tgttggattc tacttcgccc cagcccggac acacagttaa gcccattacc ggctaaaagg
    22441 ctaaaaaact tctctggatt atcgtggtaa agctgggaat caatggtggc ggttaactct
    22501 tccataggga agcacacccg ccctgtatcc cagggatatt tagtccgaag cataaacatt
    22561 gctttcagca attcacattg agcgcggatc gcgtccggtt catagccaga tatggagaca
    22621 taagccacgt ccctcattcc tgcgtcatct ttgaaagtca cgatagaagt aacatccagc
    22681 tctttttcga aagagcaagc agcatctact ggacgctgaa gtaaatcatt cgacttaatg
    22741 cgctcgagaa tccctcccca catatcattt agatattcga tatgagccaa aaccttatca
    22801 agacactctc gtgtaaacca ttcagtatgc ccgccaccga cgcttttctc ccacggcgca
    22861 ttccagggga aaaaggttgc gtgtaaagcc cgctcaagat taaccattgc caaacgcgta
    22921 ccacgataga cccgtgaaag cgcaaaatcg ggactcactt gtagtccttt aaaccgtgcc
    22981 aatggaccac atgaaatgcc gattttaaaa gtatctccgt tctctggcac cagaacgtag
    23041 aggtagtgtt gtttctcttc ttgcatatca ataccactgc ttgatgagaa ccgcgcaaat
    23101 gttgactatg cgcgaaggtt aatgtgaata gttgactatg cgcgatgtga ctacagtcaa
    23161 aagttgactg tagtcgattt aactccacca aagatcgact atgtaagata ttgtcgggag
    23221 aaacgttgac tatacgcgat gaaatgcccc taaaagccat ctcaatagcg acttgcagaa
    23281 tattgacgcc agcaaaaatc caccagcgtc aacgaatgtc gcctatagtc aacttctcgc
    23341 tatcgcatat agtcaatatt atggattgcg cttatggatc tggaagccga ttttcctgcc
    23401 gttttttatc tctgaaaatt taagatattc aatagcttcc aaatctttca tggcttttct
    23461 gataacgcta ttttgcacgc taacggatga tttgagatta agcctcgctc taaggcgctc
    23521 aatgctgaca ggtgccgggt tggcgggtag agcctcaaag aatgtataca gtaccttggc
    23581 cgtctctttg cgccctagtt tatccagcat cttcagcttc aggattcgct tatagtcaac
    23641 atagtaaagt tcagatagct gtttctgcgg ctggatctcg ataacatcaa gctcggtatt
    23701 caggctgcta tatgccaaca agttgacgtt aatgttattg agatgacctt ttgccgccgg
    23761 gaagcggaat ttgacaactg tctgctgaat gcgtgtcaga gagtcatcaa tacttttacg
    23821 gaacgccttt gaaaggcgct tacgtggata gccgcatcga tcggcaaact cggagaatgg
    23881 cagggtgatt atgccgtcat catcaggtgc gtagtcaaac aacgcggaag ttatgcccac
    23941 ccacacctta aaatcagtat ccatatccag gcgtggacca tgaatttcaa ttccctcata
    24001 gccttcctgc tcaacaattt tgaggcttga tagttcttca gttgcgttcg ttgtgtttgt
    24061 tgtaactgac gatccgcgac gtagcgccac attggtagat tttaaggttg gcacaaacac
    24121 acctaagcgc aacaaagcga tgggttgtat agtgctgttg ttattgggct tcaggctgtg
    24181 gatttctcct gtatttcctg caacttcttc aacgctaagg aagcctttac tttcttccgg
    24241 catcacggtt tctccatgtg tggcgcggcc tgacgtcaat ttggatggct gttatcaaca
    24301 gctgtgaata ttcagactct aaaatcgctt acagtcaatg tttctgtcgc gtatagtcaa
    24361 caataaatcg cgtgcagtca acaataaatc gtgcacagtc aacataaaat cgcgtatagt
    24421 caatgttgat cccatttcag gccagtaatg acgcggctta cagcgatccg ggatcttctt
    24481 tggatcttcc taggttctct ttaggatctg tttattggat ctatgctgtg gataagttga
    24541 ataaaccggc caacatagcc ggttggaagg aagggtatta ttctacgctt tcgataagaa
    24601 gaccatgttc ataacattta agatcatcgc cttcgtacag gaattggtat ccaataccac
    24661 cattttcatg gacattaggg aataactcat aactcactga agagcaaatc acaccaatgc
    24721 aacgatcaac gccttctcgt tcttcagtgc tgaaaaaatc ctcttcggta agaacatgag
    24781 tacattgctc atcagcatag gtcggaaata catgctcgat gcaatccggg tgttttaaac
    24841 caagctgatc ggcaagctcg aaagcatgac ggtattgttc agatcctggc ttgccaacag
    24901 tgatgtgctc aattttgtag attgaagtcg ctttgttgat agtttgcttt actgttactt
    24961 tatcagacat aaaaatccct tttagttacc gctgatagcg cggttgtaat cattaacgtt
    25021 gcgattcttc ctgttaatcc ccatcagcat cgtttctgta tcgaggatat acgctggcag
    25081 atcatcaaaa tattcactgc taaactctgg catcctgcac ataaatgcac tttttggggc
    25141 agggtggtta acctttgtcg gcgtcggcgt taaattcgct gatcgactcc cggagcaacc
    25201 gctgagtgtc agcaggaata cgctggcgaa cattacccgc cgcaacaagt tgtttctgaa
    25261 cttcagcttt tcgttccatt tgcctgtcag catacttggc ttgttctgat tcatttttca
    25321 cttcctggct gtgaaaatgt tgctctgctt tgttcatcgt ctcaatggtc tggttaagat
    25381 ccattattga cttatcacgt tccttaacag cctgatcaag actgccaatt ttctccatgg
    25441 cttgctttag ctgatgacgt tcccacgcaa acccagcacc aacaagtgcg caaatcagaa
    25501 caagaacacc agtagcagca agtttctcct tcaaagacaa agctgttttt aatgtagaaa
    25561 agaatgacat gtcttcctcc tgaagaaaaa ttatcaatga agtcctttgt tactgtgccg
    25621 ctttgtttaa ttcatcaaga acagaatcag gaaccaaagc ggcgattgcg ctggctgtgc
    25681 tggccttatt tgctgatgct tccgcaagcg cggtaccgat agcatggtta taagcagtta
    25741 tggctacgtt ggcgctttca ttcgctcgtt catactgctg ttgtaacgca gttgtgggtg
    25801 ctgttgtctg gttgaaaaca accccaaact gttcagttgc tactttcaga gattcaattt
    25861 gctcttctgt tagtgctggt gggggagtgg cagtgccgcc gcctgaacca gagcctgacg
    25921 agcttcctga gccagtgtta agggtctggt taatctcccc catagcagcg actaaacttg
    25981 atgtattaag cgcgtttaca gcgtcctcaa gcgatttagt aatagtcaca tcaccaatgg
    26041 caatagagat cggcagttct gaaacttctc gctcattagc acgacagtaa acatcccaac
    26101 caatatcgag ttgaaggagc attgacagat cagcataacc agccaacagg tccgcgtgct
    26161 gagttgccag ccctccaata ttcgttaaac cggttgcggt tgttctgatc gttgaaacat
    26221 agctggtaat agtgtcggga tagacaattg tatccagaat taatccggtc aattcttctg
    26281 caagcagttt tgctgtgtta gcactgtttc gtgccgatgt tatggcacca ggtgttttca
    26341 tcccaccggc ggcggccaat tttttatatg cggataactg gtagtctttt tccagcatga
    26401 tatctcctaa cttacctgaa ccaggccgtc tccggctgca acggtagagc cgcatgaaac
    26461 aggatcacca acgcatacga tccctttccc gttgacggta aaccatgccc tggttgatat
    26521 agcttgcccg ccgtgcgtac tgtttccatc ggtatgctgt gcatattgct taccatcaac
    26581 taacacttct actccgttga ctttaagtag tggttcactc tctacaggag gcctggatgg
    26641 gaatcctccg tgccccgaac aaatgctgtc ttttgttgca atacttgcca cgttatcacc
    26701 aatgatttgc tctgattttc gttattttaa cttaggttgt ttgtggtcta catggcgttt
    26761 acttattata aaattgctct aataaatatt gttttttatg tcgtgttttc ggtaccattc
    26821 agccatcgcc cttcaatggg catttgtttg gagtcgtcag atgcagatgg agctaataag
    26881 ccgcaaggag ttcgatagcc gtgtaaccag cggtgaactc gacaacttgc aggctatcaa
    26941 ggtgaaagaa ggcttttgcc tcattgggaa tcagagcgga acaaatcgcg tttttatgct
    27001 tcgccgtacg gatttgaagc catttgtctg gaagaacgaa attggtccca gctcatacgc
    27061 tcaaacgagg gggtgccaca acctggcctt tttctacaaa gacgagcttt ctgtggttga
    27121 tattcaaggg ttacaacatg tttaagcact ggaaaaacat tactatttat aaactttctc
    27181 gtgaggcgga tctgaccgac ttagaagata aaaagaaaat gatccttttc acgccatgcg
    27241 gtagtcagga tatggccaag ttcggttttg tatcgccatt tggtgataat tccgaagtta
    27301 tcgctatgca tggaaatggt tttatccttg ttgaagcaaa gcgcgaaaca aaaattcttc
    27361 ccccgccggt tatccagcga gctattcaag aaaaaattga aaaacttgag caagaacaag
    27421 cgcgtaaact gaagaaaaca gagaaggact ccctgaaaga cgaagttctg cattctcttc
    27481 tgccacgggc tttttcaaag ttttctgtta tccaggcgat ctacgacggt tcaactaaac
    27541 gtatctatat caatgccagc gcgcggcagg ctgaggatat gctcgcgctt atgcgtaagt
    27601 ctctgggttc tcttcctgtt gttcccctga gtgttgaaaa tcccattgaa ttaacgctga
    27661 ccgactgggt acgtgatggt agtgctccac agggatttca aatgggggat gcggcagaac
    27721 ttaaggcagt gcttgaggat ggcggtattg cccgagtgaa aaagcaggat ttgggaagcg
    27781 atgaaatttc cacacacctg gaagctggca agctcgtcac taagttggca ctcgactggc
    27841 agaaccgcat taaatttaca ctggaccata acttcagcct taccagcgtc aaatttgcgg
    27901 atgaattgct tgagcagaac tctgatattg atagtgaaga tgttgcgcag cgactggacg
    27961 cagatttctt cctgttgacc agtgaaattt cgtgcctggt tgatgctctg gtaaatgccc
    28021 ttggtggaga ggctaagcag tgaaagagct gtgctatgga tctgtttgca gtggaattga
    28081 agccgcgagt attgcctggg aaccgttggg tatgcgtccg gcgtggtttg ctgaaatcga
    28141 gtcttttcca tctgccgttc ttgcgcaccg ctggccccat gtcgcgaacc ttggcgacat
    28201 gacaaaactc gcccaaaaag tccaggctgg agaaattgaa gcccctgatg tgctcgtcgg
    28261 gggtacgcct tgtcaggcat tcagtatcgc gggcttacgt ggtgggcttg atgatgaacg
    28321 cggcgcgcta actttgaagt atgtggagct tgcaaatgca attgacgaca aacggtctga
    28381 gtccttcctc aaaccgacag ttatcgtctg ggaaaatgtc ccaggagtcc tgtcatcagc
    28441 agacaacgcg ttcggatgtt tccttgccgg attggctgga gaagatgcgc cattcgaacc
    28501 tggtgatcga cctgaatcac gaaaaagtaa cacgttctgg cggtgggatg tcaaaaccgg
    28561 ttgccatgct ccaaagtggc cgcagtgtgg ttgtatttat ggaccgcagc gaaaggtggc
    28621 ctggagaatc cttgatgccc aatacttcgg agtggcacaa cgacgccgac gcgtgtttgt
    28681 tgtcgcaagt gctcgaacag acctcgatcc cgcaacggta ctttttgagt tcgaaggcgt
    28741 gcgccgggat attgcgccga gcagaggcga ggggaaggaa gttgccggaa atgttggaaa
    28801 tggcattaaa agcgggagcc attgggataa ccctgtaaat cctcacccga cacttaacca
    28861 gtcacacaat actggtggga ttggtgcaag taaccaagag atttttgcgc agcgaggcag
    28921 cggacttgtg tattgcatgg cgcatgggca agggggggca gaaataaaaa atgatgggac
    28981 atcacctact cttacttgta atcatgaagc tccaattgtg ttttcttgca agaaagctat
    29041 tgcgtttaca agcaaaaatt atgggcagga tgcgggtgtt gagatttccc caactttacg
    29101 agctggtaac tctgctaata gcaatcaaaa cgcgggatgc ccaccagctg ttgcgtattc
    29161 gctacaacat gcacaaattg ggcgaaaaga tgatgctgga ccgcagggta aagggtggca
    29221 aaaagagata agttttactc ttgattcccg cgcaacagct gacgccgttg cgttatcttt
    29281 ttgggggcag gaaagtattg aaaaaggcga attaggtgag aatggttact tacctcatat
    29341 gatgagcgtt cgccgtctga ctcctgtcga atgtgaaagg ctgcaaggtt ttcctgatgg
    29401 acatacgttg attccaacgg gaaagcgtaa gaaagttact tcagatgaac ttatatacct
    29461 tcgcaatcac gatcctgatt taagtgaaga agaggccgcc atgcttgcag ctgacggacc
    29521 tcgttacaaa gcgatcggca atagtatggc gataccagta atgcgctgga ttggcgagcg
    29581 gattaccaag gctgcatgtc ggcagaaaga aggaagtgaa acaaaagagc gaaaagttaa
    29641 accagcggca gaattcgaac ggtccatatt caaatgggct ggtggaaaat ttggtgttct
    29701 ggaacaaatc tttcgctatt tgccagaagg gaagcgcctg attgaacctt tcgttggtgg
    29761 cggagctgtc ttcatgaatg ccggatacca ggaaaatctg ctaaatgatg tgaatgctga
    29821 cctgattaac ttttacaaga ctctgcaacg cgaggcgcat tcacttatca ctctggcaca
    29881 tcgtttcttc caggactaca acacgcagga aggatacctg gcagtacgga atgcgtttaa
    29941 caaacaagtc tatgatgatt tacatcgcgc agcggcgttt ttgttcctga accgacattg
    30001 ttttaacgga ttgacgcgtt acaaccaagc cggtgagttc aatgtcggtt atgggaagta
    30061 taaaactccg tatttcccat tgcaggagat ggaagccttc cttggtgcgg aagggcgttc
    30121 tgagtttgta tgtggtgatt ttgctgcggt gattgaagct gccggagaag gagatgtcat
    30181 cttttgcgat ccgccgtatg aaccgctccc aaatacagag ggattcacga actattccgg
    30241 tcatgacttt aagtttgaag agcaaaaacg cctggtgtct ctgttgacgg atgctcatcg
    30301 ccgaggtgca aaggttctca ttactaacag tggcgcgcca aacatcagag aactttatca
    30361 ggacagtggc ttcagagtgg aacctctttt tgccagacgt tctgtgtctt gtaaggggga
    30421 cactcgaggt gttgctcatg acgttatagc aatattgctc taataaattt attagtgtaa
    30481 tatcgcctca atgaatcgtg atttatagag cgatttagct gttagccgcg acaggcgcgg
    30541 cggcaagtat ggcggggtag tgactccttc cccctcatga cgccgagttg ccaggttgac
    30601 catacgccta agtggcaaca ccgaagtgcg ttacgagctt ccagtttgcc catcttcggg
    30661 tgggcgtttt tttcagggtt ttcgtcatgg ttagcgactt tgcggcggtt tagaaactga
    30721 ccattaaagt aaatgcaaac gatgatctga tgatggtagc ggcctaagaa gccagacgcc
    30781 acggggtatg agtcgtcccc cgtcaaaaaa tcgaccgcag agtgtccccg tctgtgtatt
    30841 agggaacggg gaggcacaac aggtaagggc gctggtgtga ttaaccagat gaacgagaag
    30901 gggccatctg ttggtcagcg tcctttcctg ttgcgtcttc ttttcagcgt aacagcggtg
    30961 cttaacagca ctttgggtac agttccacga atttacgggt atatcccgtc atgctgaagg
    31021 cgctaatcac gctggaagcc agggttgtgc atcccctgtt accgaattgc agccagggcg
    31081 cggtgcgccg aaaagcatac ggaggtggaa gccctcgccg gagacgtacc cggcaagtga
    31141 tggtgtagct cagcggttag agcggttgac tgttaatcaa cgggtcgatg gttcaaatcc
    31201 atccaccatc gccaatgccg gtttagctca gttggtagag cgcctgcctt gtaagcagga
    31261 tgtcagcggt tcgagtccgt taatcggcac cagcacaaca ggtaagggta ttttgcgacg
    31321 tcggagatcg ccgagcttgg cagagggttc gaatccctac gaagtaccct taccgttgtg
    31381 atgaatgcgc aggctgatgc gcgaaagaca ttgcagctat tgcggaaaag agctgttcgg
    31441 cggggcaatt aaacgcccgt gagagtctga aataaccgca agccggagat cagcaccggt
    31501 catcacaaca caacaggtaa gggcattctc ccttatgggg cttggcttaa atgcatcgag
    31561 tgctcttacc gttgtgatga agtgcagctc tttgaagcaa ccagaagata agcatctggc
    31621 ttcacaacat aaaccgcagg aacgaccaat aaacggtagt ccgtatggag aaccccccgt
    31681 tgaggaagag gcctggccgg aaccgtaacc ggcactacaa cgttgagaac actggcgtaa
    31741 cggggtcata tcccaatcta cgaataaatg ttgcgttgca gcgtgacaac cagtgttctc
    31801 aacattgtgg tgaatgcaca ggctgatgtg ccgcaactac agtagtgcgc gctttgcggg
    31861 gcttgctaca accctgtgtc ggagttcagc accgaccatc acagtttgat tctctggcat
    31921 gagcataacg ctgaaataag tccagtctgg tgcggcccga tcacccgccg ttagctccac
    31981 gaaacggagc acgtaacagg taagagtatt ctcctgtaac gggttcatat cccaatctac
    32041 aggtccacca ggaatgctct ttccgttgcg gtgaatgcgg ctaagcgcac gcggggaaat
    32101 ggttatatct gtccattatt tctccttatt tccccgtcca cggtggataa ccagccaaag
    32161 gacaccggga ggcacccggc accgcaacct tatttcccaa ccagtaatga ggttaataaa
    32221 tgctcggcat tctcaaaaag aaattccgca aagcggctgg cggagtcaag aagatggaaa
    32281 accgtgatgc ggtggaagcg actgtctggg gcgcatattc cattgcatac tctgacggca
    32341 cctgcgatgc gaaagaaatt gcagtattgg aaaaaaccat tgcagcactt cctgcctttg
    32401 cgccgttctc cggtgaaatt gcccagatga gcgccaatat tcgcgctcaa tatgaagcct
    32461 cgccgcgccg agcgaatgcc caggctttac gtgaactggc tgacgtgtct ggaactaatg
    32521 atgcggtaga tgttctgtgc ctatgcattg atattgctga ccaggatggc attggggcag
    32581 aagagcagga gcaattgaag aaaattgccc aggctcttca attgccactg gaacagtata
    32641 tctgatggtt ataaaagcac gtctaattct ggctttggtt tttctcgtgc tatctgtgct
    32701 ggtggatttc accagcacaa tcctgtcggt tttatccgac ggggcgttgg tagcagtagc
    32761 tgtaacattg gtatggccga tatttaaacc agcctcgaag gatcagtgat gagcttctgg
    32821 gattttgcag ataagtatcc aattgttctc attatcattg ttgccatagt tgtaggcggt
    32881 attgttagcg ccattgaagc acttaggaaa cagtaatccg gccctttagc tcagtggtta
    32941 gagctggcga ctcataatcg cacggtcacc ggttcaagtc cggtaggggc caccatattt
    33001 ggttgtaaca cggcgtctgg cacatgcgtc gttagcggtc tggtgacgtt aaaagggggg
    33061 aaccttgccc ctagctcagg caacgaacca ggtagccgga atgtgcaagc caccgtttgt
    33121 tgtttctcgg gtaaagggat tcaccatcct ggcgattcgg tgtgacagcc gggaagagtc
    33181 cggcgcatta atcctgattt tctggtgatg actcatatcg ttaggagtga tttgagtatg
    33241 ccgattatat ctgacattca gcacgcctgg gtggagtgct aatgtctgca tcccctcttg
    33301 aatccatgcc aaattccctt agtgcagaac aagctgtact tggtggctta atgcttgata
    33361 actgccgctg ggatgaagtt gcagatcgta tagttgctga tgatttttat accagtgctc
    33421 atcgtgaaat tttcagtgag atggagaggt tattaagtca tggcaaaccg attgatttga
    33481 taacacttgc tgaagcactt gaacagaacg gtaaattaga acgcgccggt ggttttgcgt
    33541 accttgcgga gatgtcaaag aacacgccca gcgcggcaaa tatttgtgct tatgcggata
    33601 tcgttcgtga acgcgcggtt gttcgtgaaa tgatttccgt cgcaaatgaa atagccgaag
    33661 ctggatatgc gcaggatggc aggggcagca atgaattgct ggatatggcc gagcgccgcg
    33721 tttttgaaat agctgaaaaa cgacaaaaga gcggtagtgg tccaaaagat atcgccagca
    33781 ttctcgatgc aacggtatct cgcatagaag agttgtttca gcgaccacat gatggtgtaa
    33841 cggggcttga tacgggattt accgatctca ataagaagac ggcaggactt caggcgtccg
    33901 atctcattat tgtcgccgcc cgcccatcga tggggaagac tacgtttgcg atgaatctcg
    33961 tcgaaaatgc cgcagtccgt aacgataagc ccgtattggt ttttagcctt gagatgccga
    34021 gccaccagct gatgatgcgc tcactggctt ctcttgcacg cgttgatcag tctcgtattc
    34081 gaacagggca acttaacgac gaggattggg cgcgggtttc tggcgcaatg ggtattctgt
    34141 tggacaagca gaatattttt attgatgact caagcgccct gacacctaca gagcttcgtt
    34201 cccgcgctcg tcgtgtttat aaagaaaatg gtggtttgag catgattatg atcgactacc
    34261 tgcaacttat gcgcgtcccc gagctgcaag ataaccgaac gctggaaatt gccgagattt
    34321 ctcgctcact gaaggcgttg gcgaaggaat tacaagtacc ggtggtggca ttgtcacaac
    34381 ttaatcgatc gcttgaacag cgtgcggaca aacgaccggt aaattcagat ttacgtgaat
    34441 caggagcaat tgagcaggac gcagacctga tcatgtttct gtatcgcgac gaagtttatc
    34501 acccggatag cgaaatgaag ggcattgccg aggtaattat cggaaagcaa cgaaatggcc
    34561 caattggcac ggtgagattg gcttttaacg gccaatactc acggtttgat aactatgccg
    34621 gtgctgactg gcaagaggat tattaatgca atggaatgag gaaaagccga tgaacatcct
    34681 gatcattggg cgaaaatttg aagctatcag tgatgtgaaa acatatacgg aaatgtgggc
    34741 ttataacctg gcctgcgcct ttagtgaggc tggggtaaca ttgcaatacc atcgtccata
    34801 ttcccccggc gtcgaaagcc cggaggatta tgttgaagct gtgttgaccg ctgcgacctc
    34861 gtgttctgcg aaagccattt tagcgccagg attgcggtat tttactacgg tgcccaggga
    34921 aataggcgtg caactgcgtc gtcgattcac tggatgggta gcccaggtat acgacggttc
    34981 tatgctggat tcggcaccag tcgatattac ttttactgtc cgcgatgata cctggcggta
    35041 cctggataat cccggcaggt tagagcgtca taatcgcttt aacaaacatg ttggatgggc
    35101 agcgaatcag gagctgttcc atctggaaac caaaacagac gatgttctgc gtatttttgt
    35161 agaccacgct gcatttgatg ttagtgggtt tgatcactcc ttaagtatcc ttatgaacct
    35221 tcagcgtctg accgttccgt atgaggccag aacgttgact gatgatggat tggttaccat
    35281 tgatccgggg aatatttcgg taactccata cagacggacg ccggtgccag caaccgaatt
    35341 tgcagctgaa ttgcgtaaga gtgacgtttt tatcgttacg catcccgaaa gccttggatt
    35401 aactgttctt gaggcggcaa tgtgcggggc gttggtatta acgcctcccg attgccttcc
    35461 gccagatcgc ctggctttgg tgaaccatat ggttatcaag tcgcggattg attgggatga
    35521 ggttattgct cgcgttgatc gcgtgaaaaa tgctgaaaag gtccagtgtc acacctggtc
    35581 ggcaattgcg gaaaagatgc ttgagacgtt tatcacgcag aaaccgtcgt gcggtaacgg
    35641 ataaaaattg aacccattat aaaagaaaag cccgatcgcc gggcttttct taagccttgt
    35701 caacagagac ttgagcggct tttatggata gattcccgct ggcctctatc gccatacttc
    35761 cccccgcctt cagggcgaca tccgcgcctg actttatatc gagatttcct gcggaagaga
    35821 tgaatgccgg accttgagaa atggcatata actccccggc ctcgttgaac ccgattgttg
    35881 ttccactttt caagtgcgta acggcccagg ctccgcccgc cgtccggacc tccattagtc
    35941 cgttccgcga cgaaataaag tcttttttgg cgctggttga tggttgtgct ggtgcacctt
    36001 caacttcagg cggtacatag ccttcacctt gtcctgacgc ttcaggtggc acattgggag
    36061 cgccaccgga tgcatcctgt gcataaccga ttatcaatgg ccatcgagaa tccccattgt
    36121 agggaaattc tacccatact ttatcgccgg gcagaaatgg cgaaaacgtg tttgcattgg
    36181 acaatatggc ttctgcccac ggcaatgagg catctggtag cccatccatc atgccgacaa
    36241 cgcgtatttg cgtacgcatc agacctttag ggtcatcgac gcttaccact acagcccgat
    36301 acttccctgt caaactaccc attcaccact cctaactgtg cacggctg
//
LOCUS       DAMZET010000028        35906 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.028, whole genome shotgun sequence.
ACCESSION   DAMZET010000028 DAMZET010000000
VERSION     DAMZET010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.028"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..396)
                     /locus_tag="POB34_001510"
     CDS             complement(<1..396)
                     /locus_tag="POB34_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HDI5918486.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQ"
     gene            1008..3713
                     /gene="malT"
                     /locus_tag="POB34_001511"
     CDS             1008..3713
                     /gene="malT"
                     /locus_tag="POB34_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HDI5918487.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(3756..4772)
                     /gene="rtcA"
                     /locus_tag="POB34_001512"
     CDS             complement(3756..4772)
                     /gene="rtcA"
                     /locus_tag="POB34_001512"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HDI5918488.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(4776..6002)
                     /gene="rtcB"
                     /locus_tag="POB34_001513"
     CDS             complement(4776..6002)
                     /gene="rtcB"
                     /locus_tag="POB34_001513"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HDI5918489.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSVTQKTVRQQQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            6191..7789
                     /gene="rtcR"
                     /locus_tag="POB34_001514"
     CDS             6191..7789
                     /gene="rtcR"
                     /locus_tag="POB34_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HDI5918490.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIALCRQAKSLSAAGRLLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(7771..8529)
                     /locus_tag="POB34_001515"
     CDS             complement(7771..8529)
                     /locus_tag="POB34_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HDI5918491.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(8546..9376)
                     /gene="glpG"
                     /locus_tag="POB34_001516"
     CDS             complement(8546..9376)
                     /gene="glpG"
                     /locus_tag="POB34_001516"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HDI5918492.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(9421..9747)
                     /gene="glpE"
                     /locus_tag="POB34_001517"
     CDS             complement(9421..9747)
                     /gene="glpE"
                     /locus_tag="POB34_001517"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HDI5918493.1"
                     /translation="MDQFECINVAVAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            9937..11442
                     /gene="glpD"
                     /locus_tag="POB34_001518"
     CDS             9937..11442
                     /gene="glpD"
                     /locus_tag="POB34_001518"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HDI5918494.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(11496..12101)
                     /locus_tag="POB34_001519"
     CDS             complement(11496..12101)
                     /locus_tag="POB34_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918495.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(12104..12871)
                     /locus_tag="POB34_001520"
     CDS             complement(12104..12871)
                     /locus_tag="POB34_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918496.1"
                     /translation="MQPSPAMQALIEQIYHIFRRYPVPQKFVVCCEYCLSQQEQKVLR
                     STSLRAISYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDEWKILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWAFNNQSILKERARQAIEN
                     PPKQPEQGTQHQVWVDDWIIDECLCAMYDASSESPGK"
     gene            complement(12874..13584)
                     /locus_tag="POB34_001521"
     CDS             complement(12874..13584)
                     /locus_tag="POB34_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918497.1"
                     /translation="MELVNTLFASLVGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYNVTLNIGLTSSNVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDLTVQFTAMLNPDERVIIAKRPLKKCESWIEDIYIFQVFKDAW
                     KFHNDNSLRGFRDKQAELKLYARYYTVENCAEESCRDCNSCIRPSFSLSRSAIIRLNV
                     ANARFVYQPFTRDQRARG"
     gene            complement(13599..15107)
                     /locus_tag="POB34_001522"
     CDS             complement(13599..15107)
                     /locus_tag="POB34_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDI5918498.1"
                     /translation="MMQNRKWILTSLVMTFFGIPILAQFLAAVIAMLGVGLAGIIEVC
                     NIFITPTIYLLLNIFMLALGALMLFFSGRVWADDSAPEKREIAVWRQCLFLVPALLTL
                     GVWIIALHLADYQFRQMGAGWLADLMLPWLGVLLASLVGGEYWWLVIIPVGAHISFSL
                     GYGWPTRYPLTGTSGLRCRNSLLFILLMLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVLPEDFHEWEYLA
                     NSRTPEAYNKIVKGNADIIFVAQPSGGQKKRAEESGVTLIYTPFAREAFVFIVNVDNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENMTSETQKLVEWFLTPQGQSLVEDVGYVPMYKTLH"
     gene            complement(15232..17679)
                     /gene="glgP"
                     /locus_tag="POB34_001523"
     CDS             complement(15232..17679)
                     /gene="glgP"
                     /locus_tag="POB34_001523"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HDI5918499.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(17698..19131)
                     /gene="glgA"
                     /locus_tag="POB34_001524"
     CDS             complement(17698..19131)
                     /gene="glgA"
                     /locus_tag="POB34_001524"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDI5918500.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(19131..20426)
                     /gene="glgC"
                     /locus_tag="POB34_001525"
     CDS             complement(19131..20426)
                     /gene="glgC"
                     /locus_tag="POB34_001525"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDI5918501.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(20444..22417)
                     /gene="glgX"
                     /locus_tag="POB34_001526"
     CDS             complement(20444..22417)
                     /gene="glgX"
                     /locus_tag="POB34_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HDI5918502.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(22414..24600)
                     /gene="glgB"
                     /locus_tag="POB34_001527"
     CDS             complement(22414..24600)
                     /gene="glgB"
                     /locus_tag="POB34_001527"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HDI5918503.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPWAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(24873..25976)
                     /gene="asd"
                     /locus_tag="POB34_001528"
     CDS             complement(24873..25976)
                     /gene="asd"
                     /locus_tag="POB34_001528"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDI5918504.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            26169..26762
                     /gene="yhgN"
                     /locus_tag="POB34_001529"
     CDS             26169..26762
                     /gene="yhgN"
                     /locus_tag="POB34_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HDI5918505.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(26819..28159)
                     /gene="gntU"
                     /locus_tag="POB34_001530"
     CDS             complement(26819..28159)
                     /gene="gntU"
                     /locus_tag="POB34_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDI5918506.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     FAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(28163..28690)
                     /gene="gntK"
                     /locus_tag="POB34_001531"
     CDS             complement(28163..28690)
                     /gene="gntK"
                     /locus_tag="POB34_001531"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDI5918507.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(28829..29824)
                     /gene="gntR"
                     /locus_tag="POB34_001532"
     CDS             complement(28829..29824)
                     /gene="gntR"
                     /locus_tag="POB34_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HDI5918508.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(30048..30743)
                     /gene="yhhW"
                     /locus_tag="POB34_001533"
     CDS             complement(30048..30743)
                     /gene="yhhW"
                     /locus_tag="POB34_001533"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HDI5918509.1"
                     /translation="MIYFRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPNSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(30866..31903)
                     /locus_tag="POB34_001534"
     CDS             complement(30866..31903)
                     /locus_tag="POB34_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDI5918510.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHLTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            32236..32724
                     /gene="yhhY"
                     /locus_tag="POB34_001535"
     CDS             32236..32724
                     /gene="yhhY"
                     /locus_tag="POB34_001535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI5918511.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSDHMWQER
                     LAAHPGIKQLVACIDGTVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            32834..33118
                     /locus_tag="POB34_001536"
     CDS             32834..33118
                     /locus_tag="POB34_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HDI5918512.1"
                     /translation="MMIYQEAFAKANHYLDDADLPVVITLHGRFSQGWYFCFESQEFL
                     ESGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLHDYEIKKATFGLP"
     gene            complement(33156..34889)
                     /gene="ggt"
                     /locus_tag="POB34_001537"
     CDS             complement(33156..34889)
                     /gene="ggt"
                     /locus_tag="POB34_001537"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDI5918513.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPVSYGVEEDVFHPVRAK
                     QGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRTKD
                     GNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDKYGT
                     MPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGDK
                     LVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERTPIS
                     GDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAYADR
                     SEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTTHYS
                     VVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANA
                     VGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNA
                     PRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASD
                     PRSVDDLTAGY"
     gene            35010..35450
                     /locus_tag="POB34_001538"
     CDS             35010..35450
                     /locus_tag="POB34_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HDI5918514.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(35437..35895)
                     /locus_tag="POB34_001539"
     CDS             complement(35437..35895)
                     /locus_tag="POB34_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HDI5918515.1"
                     /translation="MLSQVAERCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAG
                     MTPPLLSSFEIDALEAAQLAAPELPRGLLLDEWRDDWRELTMRLGCVSLHLNHKLLDK
                     ARVMQLKDAGLRILVYTVNKPQRAAELLRWGVDCICTDAIDVIGPNFTAQ"
BASE COUNT         8900 a         9715 c         8892 g         8399 t
ORIGIN      
        1 ctgaccgaca gttgccattg agtcgaggaa gcacgccgcc agacgtccca gaccaccgtt
       61 acccagcgcc gggtcgatct cttcttccag caggtccgtc agattgatgt cataagcctt
      121 caacgaatcc tgtacatcct gataccagcc gagattcaac aggttgttgc ccgtcaggcg
      181 accaatcaaa aactccattg agatgtagtt aacatgtcgc tgattcgcca ccggcttggc
      241 gaatggctga gcacgcagca tttcggccag tgcttcactc actgctagcc accactggcg
      301 aggagtcatt tcagccgcag aatttaagcc ataacgctgc cactgacgtg aaagcgcttc
      361 ctgaaattgc ttatcgttaa aaataggttg tgacatagga gttccacttt tcttagattt
      421 tcaacacaac gttatcgcta gtttgccagg ctcgatgttg accttcctca tcctgcgggg
      481 gattaggcag ggaggagttg cggggatgag caaggaaatg tgatctcaac cacttaaagc
      541 tagtgcaaac cacaggatta gcagcaaatc aatgcaatac agcgcagaaa atctgtatct
      601 aagtgcaaaa aatggccgtt gcgtattttc aaaaagcgga aggtaactct ataaattaag
      661 taaaggagtg aaacagtttc ataagtaaaa tatccagtgt gctccatctc attcttaata
      721 gatttattaa gatcatcttt ttagatggca ctttcatcag gaatgaagaa gaaacccttg
      781 cttaaatgaa tctgatgaac ataagggaaa ccagtattca cgctggatca gcgtcgtttt
      841 aggtgagttg ttaataaaga tttggaattg tgacacagtg caaattcaga cacataaaaa
      901 aacgtcatcg cttgcattag aaaggtttct ggccgacctt ataaccatta attacgaagc
      961 gcaaaaaaaa taatatttcc tcattttcca cagtgaagtg attaactatg ctgattccgt
     1021 caaaactaag tcgtccggtt cgactcgacc ataccgtggt tcgtgagcgc ctgctggcta
     1081 aactttccgg cgcgaacaac ttccggctgg cgctgatcac gagtcctgcg ggctacggaa
     1141 agaccaccct catttcccag tgggcggcag gcaaaaacga tatcggctgg tactcgctgg
     1201 atgaaggtga taaccagcaa gagcgtttcg ccagctatct cattgccgcc gtgcagcagg
     1261 caaccaacgg tcactgtgcg atatgtgaga cgatggcgca aaaacggcaa tatgccagcc
     1321 tgacgtcact cttcgcccag cttttcattg agctggcgga atggcatagc ccactttatc
     1381 tggtcatcga tgactatcat ctgatcacta atccagtgat ccacgagtca atgcgcttct
     1441 ttattcgcca tcaaccagaa aatctcaccc tggtggtgtt gtcacgcaac cttccgcaac
     1501 tgggcattgc caatctgcgt gttcgtgacc aactgctgga aattggcagt cagcaactgg
     1561 catttaccca tcaggaagcg aagcagtttt ttgattgccg tctgtcatcg ccgattgaag
     1621 ccgcagaaag cagtcggatt tgcgatgacg tttccggttg ggcgacggca ctacagctaa
     1681 tcgccctctc cgcccggcag aatacccact cagcccataa gtcggcacgc cgcctggcgg
     1741 gaatcaatgc cagccatctt tcggattatc tggtcgatga ggttttggat aacgtcgatc
     1801 tcgcaacgcg ccattttctg ttgaaaagcg ccattttgcg ctcaatgaac gatgccctca
     1861 tcacccgtgt gaccggcgaa gaaaacgggc aaatgcgtct cgaagagatt gagcgtcagg
     1921 ggctgttttt acagcggatg gatgataccg gcgagtggtt ctgctatcac ccgctgtttg
     1981 gcaacttcct gcgccagcgc tgccagtggg aactggcggc ggagctgccg gaaatccacc
     2041 gtgccgccgc agaaagctgg atggcccagg gatttcccag cgaagcgatt catcatgcgc
     2101 tggcggcagg cgatgcgctg atgctgcgcg atattctgct taatcacgcc tggagtttgt
     2161 tcaaccatag cgaactgtcg ctgctggaag agtcgcttaa ggccctgccg tgggacagtt
     2221 tgctggaaaa tccgcagtta gtgttattgc aggcgtggct aatgcaaagc caacatcgct
     2281 atggcgaagt taacaccctg ctagcccgtg ctgaacatga aatcaaggac atcagagaag
     2341 gcaccatgca cgcagaattt aacgctctgc gcgcccaggt ggcgattaac gatggtaatc
     2401 cggatgaagc ggaacggctg gcaaaactgg cactggaaga gctgccgccg ggctggttct
     2461 atagccgcat tgtggcaacc tcggtgctgg gtgaagtgct gcactgcaaa ggcgaattga
     2521 cccgctcact ggcgctaatg cagcaaaccg aacagatggc acgccagcac gacgtctggc
     2581 actacgcctt atggagttta atccagcaaa gtgaaattct gtttgcccaa gggttcctgc
     2641 aaaccgcgtg ggaaacacag gaaaaagcgt tccagctgat caacgagcag catctggaac
     2701 agctgccgat gcatgagttt ctggtgcgca tccgagcaca actgttatgg gcctgggcac
     2761 ggctggatga agccgaagcg tcggcgcgta gcgggattga agtcttgtcg tcttatcagc
     2821 cacagcaaca gcttcagtgc ctggcaatgt tgattcaatg ctcgctggcc cgtggtgatt
     2881 tagataacgc ccgtagccag ctgaaccgtc tggaaaacct gctggggaat ggcaaatatc
     2941 acagcgactg gatctctaac gccaacaaag tccgggtgat ttactggcaa atgaccggcg
     3001 ataaagccgc cgctgccaac tggttgcgtc atacggctaa accggagttt gcgaacaacc
     3061 acttcctgca aggtcaatgg cgcaacattg cccgcgcaca aatcttgctg ggcgagtttg
     3121 aacctgcgga aattgttctc gaagaactca atgaaaatgc ccgcagtctg cggttgatga
     3181 gcgatctcaa ccgtaacctg ttgctgctta atcaactgta ctggcaggcc ggacgtaaaa
     3241 gtgacgccca gcgcgtgttg ctggacgcat taaaactggc gaatcgcacc ggatttatca
     3301 gccattttgt catcgaaggc gaagcgatgg cgcaacaact gcgccagctg attcaactca
     3361 atacgctgcc ggaactggaa cagcatcgcg cacagcgtat tctgcgagaa atcaatcaac
     3421 atcatcggca taaattcgcc catttcgatg agaatttcgt tgaacgtctg ctaaatcatc
     3481 cggaagtgcc agaactgatc cgcaccagcc cgttgaccca acgtgaatgg caggtcctgg
     3541 ggctgattta ctccggttac agcaatgaac aaattgcggg cgaactggaa gtggcggcaa
     3601 ccaccatcaa gacgcatatc cgcaatctgt accaaaaact cggcgtcgcc caccgtcagg
     3661 ctgccgtgca gcacgcgcag aaactgctga agatgatggg ttatggggtg tgagtttagc
     3721 cggataatgc attggatccg gtttacatct ttacatcatt caatactcac ccgcgttacg
     3781 ccatctgttt ctatcaaacc aaaccgcacc ggcaagaaac gctccaccac cgcgatattg
     3841 gtcagcagat ggcatgaagg atgggcgacc ttaaattctc ccgcgcccgc cagcgccatc
     3901 ggcagtacca gttggtcggc gagatattcc cccaccgccg ctgggcttgc caggtagcgt
     3961 ttcacctctt tcactaactg cgcggctacc acctcggcac tgacgcgctt ttcaccgacg
     4021 acgaaaaagc gttcagtgat attttcactt tcgacttcca gcgagacggt attacccggc
     4081 ccctggtcgc gcggcaggtt atgaatattc tgttcatgca gggaaaaact ccccgccagt
     4141 gtagcgattt cacgctcagc aacatggcgc ggcacaccag ctaatagaac ttctccacgc
     4201 atctgcacaa tgttcccgcg ctcgccaagt tgcaaggtgt taaacgatgc cacgggcgag
     4261 acttccgttg ccaccacgcc gcctccggca ggataaaaac cgtgacgtaa cagcgtggtt
     4321 tgctgatgaa ttcctatttt cgccagcagc ggctccagca cccggcggat aaaatccgca
     4381 ggcggggccg acgggttatc ggtgcctccg ctcacttcaa cacgcgaagg tccatcggca
     4441 aaccacagcg cgggcagcac cgtttgcagc accagcgtac agcttccggc actaccgata
     4501 gcaaagcggt actcgccgcc gcgcacggtg ccaggccgga agaccagacg ctgcgacccc
     4561 agctccgcgc cttccaccgt tgccctacaa atttccgcag ccgcttttac cgcggtcaga
     4621 tgctggcgca acagccccgg tttcgctcgc ccggcacgaa tgctggtgat ggtaaatggc
     4681 tggccggtta tcatcgacag gctcagcgcc gagcgcagga tctgcccgcc accttcgccc
     4741 tgtgcgccat ccagcgcaat catccttttc atcatttatc cttttacgca caccacctga
     4801 cgcagggtat agataacttc caccagatcg ctttgtgccg ccatcaccgc atcaatatct
     4861 ttatacgcca tcgggatttc gtcgatcact tcggcatctt tacggcattc cacatgcgcg
     4921 gtggcacgaa tttgatcttc cacgctgaac agttttttcg ctttagtacg gctcattacc
     4981 cgcccggcac cgtggctgca cgaacagaac gactcttcat ttcccagccc acggacgata
     5041 aagcttttcg ctcccatcga accgggaata attccatatt gaccagcacg cgcagacacc
     5101 gcgcctttac gcgtcacgta gatctcttcg ccaaagtgct gttctttttg cacatagttg
     5161 tggtgacagt tgatctcttc catcgccagc gtttgctgtt gtcttaccgt tttctgcgta
     5221 acgctctgca acgctgttac tacgttttcc atcattgcat cgcggttaag gctggcaaaa
     5281 agctgcgccc aggccaccgc tttcaggtaa tcatcaaagt attccgtacc ttccataaag
     5341 tacgccagat cacgcgacgg caacgtttca agctggtcct gcatctcttt ttgcgccaga
     5401 tcgataaagt aagtcccgat ggcgttacca attccgcgtg aaccggagtg cagcataatc
     5461 cacacctgat ccgactcatc aagacagatt tcaataaaat ggttacccgt tcccagcgtt
     5521 cccagatgtt tatagttatt ggtattcagg aaacgcggat atttttgcgt taaccactga
     5581 taaccggctt ccagctcagc ccatttagca tcgacgttaa caggtggatt ttcccaggca
     5641 cctttatcac gtttacaacg gccagtggta cgcccgtgcg gcacggccgt ttcaatcgcc
     5701 tgacgcagct ctgccaggtt ttcaggcagg tcttccgccg ttaacgcggt acgcagcgcg
     5761 ttcattccac agccaatatc cacgcccacc gccgccggaa taatcgcccc tttggtcggg
     5821 atcacgctac caatggtgga acctttaccc aggtgtacat caggcattac cgcaatatgt
     5881 ttgaaaataa acggcatctt cgccgtatta ataagttgct gacgcgcatc ggcctctacc
     5941 ggcacgcctt tggtccacat ttttaccggg gcattttcag tggtcagtaa ttcgtaattc
     6001 attttgtttt tctcttttcg ttgtttgctg tcctgataat tgcaaccgtc gtgccagaaa
     6061 attaaataag cagcttaatt ttttaattca ttgttttaaa aaagattata tctttacgcc
     6121 cgtaaccgga gatttcccgc aaagccaatt taccgataat gaaatatcgt cttttataag
     6181 gatatctaag atgcgtaaaa cagtggcttt tggctttgtc ggtaccgtac tggattatgc
     6241 cgggcgcggc agtcagcgct ggtcaaaatg gcgtccgaca ctctgtttat gccagcaaga
     6301 atcgttggtc atcgatcgac tggaattgtt gcacgacgcc cgctcgcgct cgctatttga
     6361 aacgcttaaa cgcgatatcg ccagcgtttc gccagaaaca gaagtggtga gcgttgagat
     6421 tgaactgcat aacccgtggg atttcgaaga ggtctacgcc tgcctgcatg atttcgcccg
     6481 tggttacgag tttcagccag aaaaagaaga ctatttaatt cacatcacca ccggtaccca
     6541 cgtcgcgcag atttgctggt ttctgctggc agaagcgcgt tacctgcccg cccggctgat
     6601 acaatcttca cctccgcgca aaaaagaaca gccgcgcggc gcaggtgaag tgacgattat
     6661 cgatctcgat ttaagccgtt ataacgccat cgccagccgc tttgccgagg aacgccagca
     6721 aacgcttgat tttcttaagt ccggcattgc cacgcgtaac ccccacttca accgcatgat
     6781 tgagcagatc gaaaaagtgg cgatcaaatc ccgcgcgccg attctgctta acggtccaac
     6841 cggcgcgggc aagtcatttc tggcgcgacg catcttcgag ttaaaacagg cgcggcatca
     6901 gtttagcggc gcgtttgtgg aagtgaactg cgccaccctg cgcggcgata ccgccatgtc
     6961 gacgctgttt ggtcatgtaa aaggcgcgtt taccggggcg cgggaatctc gtgaaggttt
     7021 attacgcagc gccaacggcg gaatgttgtt tcttgatgag attggcgaac tgggcgcaga
     7081 cgaacaagca atgctgctaa aagccattga agagaaaacc ttttacccgt ttggcagcga
     7141 tcgccaggtg agcagtgatt ttcagcttat cgccggaacg gtgcgcgatt tgcgccagct
     7201 ggttgccgaa ggcaaatttc gcgaagatct gtacgcgcgg atcaatctct ggaccttcac
     7261 cctgccgggt ctacgccagc gccaggaaga tattgaaccg aaccttgatt atgaagtgga
     7321 gcgccacgcc acactcactg gcgacagcgt gcgttttaac accgaagcgc ggcgcgcctg
     7381 gttggctttt gcgacctctc cccaggcaac atggcgcggt aactttcgcg aactttctgc
     7441 cagcgtcacg cggatggcca cctttgccac tagcggacgc atcactctgg acgtggttga
     7501 agatgagata aaccgtctgc gctataactg gcaggagagc cgcccctccg cgcttacggc
     7561 gttgctgggc gcagaggcag aaaacatcga tctcttcgac cgtatgcaac tggaacacgt
     7621 gatagctctc tgtcgccagg caaagtcgct ttccgctgcc ggacgcctgc tttttgatgt
     7681 ttcgcgccag ggcaaagcca gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg
     7741 ttttggtctg acgtgggaag ccgtgcagga tcagcacagc tccagttgaa tatgatggtc
     7801 cgtcagcacc tgcattacgc ttactggcgg cggggcgtcg gtgtagacgg catctaccat
     7861 gctgatgctg cccatattga ccatcgcgtt acggccaaat ttcgagtgat cgacaaccag
     7921 cataacgtgg cgcgagttct caataatggc gcgcttggtg cgaacttcgt ggtaatcgaa
     7981 ctccagcagc gagccgtcgc tatcgatgcc gcttatcccc agaatgccga aatcaaggcg
     8041 gaactgggag ataaaatcga gcgtcgcttc gccaatgatc ccgccatcgc ggctgcgtaa
     8101 ttcgccaccg gcgagaataa tgcggaaatc ttcttttacc atcaacgtgt tagcaacatt
     8161 gagattgttg gtgacaatac gcaaattgct gtgattaagc agcgcgtgcg ccaccgcttc
     8221 cggcgtggtg ccgatatcga taaacagtgt cgagccgttg gggatttgct ccgccacttt
     8281 gcgggcaatc ctctcttttt cttcggtctg ggtggctttg cgatcgtgcc acggcgtgtt
     8341 aaccgaactg gaaggcagcg ccgcaccgcc atggtgacgc aggatcaggt tttgctccgc
     8401 cagctcattg aggtcgcggc gaatagtctg cgggctgacg gagaaatgct ctaccagctc
     8461 ttcagtactg acataaccct gctgtttaac cagttcgata ataccgttgt gacgttgtgt
     8521 ttgtttcatt tataaatccc tggaattatt ttcgttttcg cgcattgagc gaatcaacaa
     8581 aagccatcgc taaacccacg gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc
     8641 caaacaaatc aaaccatccg gcgacaatcc agatcagcgc aaagataatt aacccacgtt
     8701 gcaggtaaat gccactttgc ggatcgcgtt caccacgtag ccagacgtag cccatcagcg
     8761 catacaccac gccagaaagc ccgccaaacc acggcccgct gaatttttgc tgcacatagc
     8821 cgcttaacag ggcgctaatg agagtaataa caattagctt accgctaccg aggcgttttt
     8881 ccaccgcacc gccgagatac caccaccaga gcaggttaaa gaggatatgc atcagcgaga
     8941 agtgcattaa cgcgtgggtg aagtaacgcc agaactcaaa tttcagtgtt ggatcgaatg
     9001 gccaggccag ccataacatc acttcctgat cgccgagaat ttgcatggcg ataaacacca
     9061 ccacgcaggc gatcatcacc acccaggtta ccggacctgc gcgttcacgc aaggcagcaa
     9121 agaaaggata acggcgataa tgcagaccac tgccggtatg gcccgcctgc cagctggccg
     9181 ccagataacg cggatctgcc gggttttcga gaaaacgcgc cagctccgcc cgcacgcgct
     9241 cggcctggga ctcatccgcc agccagacat cgctttggtt atgttgttga atcgtaagga
     9301 taacaccctg cgtcgccatg taatcaacaa acgcctgcgc cacgcggggg ttagcaaaag
     9361 aggtaatcat caacatcgtt gctgtcgctt attccacaca aaaggggaca gtataaagcg
     9421 ttacgcgccg tacgccacct ctgcgggaaa ctgacgctgc caggcttcaa aaccgccgtc
     9481 aatgctatag accacatcgt agccctgttg cagcagatac tgcgccgcgc ctttgctgct
     9541 attgccgtga taacacatca ccatcaccgg agtgtcaaag tcgttatcac gcataaacgc
     9601 gcccagcgtg tcgttggtta aatggaaagc ctgcaccgca tgccccattg cgaaactctg
     9661 tggatcgcga atatcgacca gcaccgcctc tttttcctgc aacttctggt gcgcgacggc
     9721 aacgttaata cattcgaact gatccatgcg tctctctttc tttacaaaca agtgggcaaa
     9781 tttaccgaac agtttacgtc gaagcggcag ataaacgcca taatgttata catatcactc
     9841 taaaatgttt tttcaatgtt acctaaagcg cgattctttg ctaatatgtt cgataacgaa
     9901 catttatgag ctttaacgaa agtgaatgag ggcagcatgg aaaccaaaga tctgattgtg
     9961 atagggggcg gcatcaatgg tgctggtatc gcggcagacg ccgctggacg cggtttatcc
    10021 gtgctgatgc tggaggcgca ggatctcgct tgcgcgacct cttccgccag ttcaaaactc
    10081 attcacggtg gcctgcgcta ccttgagcac tatgaattcc gcctggtcag cgaggcgctg
    10141 gctgaacgtg aagtgctgct gaaaatggct ccgcatatcg ccttcccgat gcgttttcgc
    10201 ctgccacatc gtccgcatct gcgcccggcg tggatgattc gcattggtct gtttatgtac
    10261 gatcatctgg gtaaacgcac cagcctgccg ggatcaactg gtttgcgttt tggcgcaaat
    10321 tcagtgttaa aaccggaaat taagcgcgga ttcgaatatt ctgactgttg ggtagacgac
    10381 gcccgtctgg tactcgccaa cgcccagatg gtggtgcgta aaggcggcga agtgcttact
    10441 cggactcgcg ccacctctgc tcgccgcgaa aacggcctgt ggattgtgga agcggaagat
    10501 atcgataccg gcaaaaaata tagctggcaa gcgcgcggcc tggttaacgc caccggcccg
    10561 tgggtgaaac agttcttcga cgaggggatg catctgcctt cgccttatgg cattcgcctg
    10621 atcaaaggca gccatattgt ggtgccgcgt gtgcataccc agaagcaggc ctacattctg
    10681 caaaacgaag ataaacgtat tgtgttcgtg atcccgtgga tggacgagtt ttccatcatc
    10741 ggcaccaccg atgtggaata caaaggcgat ccgaaagcgg tgaagattga agagagtgaa
    10801 atcaattacc tgctgaaagt gtataacacg cactttaaaa agcagttaag ccgtgacgat
    10861 atcgtctgga cctactccgg tgtgcgtccg ctgtgtgatg atgagtccga ctcgccgcag
    10921 gctattaccc gtgattacac ccttgatatc catgatgaaa acggcaaagc accgctgctg
    10981 tcggtattcg gcggtaagtt gaccacctac cgcaaactgg cggaacatgc gctggaaaaa
    11041 ctgacgccgt attatcaggg cattggcccg gcgtggacga aagagagcgt gctgccgggt
    11101 ggtgccattg aaggcgaccg cgacgattat gccgctcgcc tgcgccgccg ctatccgttc
    11161 ctgactgaat cgctggcgcg tcattacgct cgcacttacg gcagcaacag cgagctgctg
    11221 ctcggcaatg cgggaacggt aagcgatctc ggggaagatt tcggtcatga gttctacgaa
    11281 gcggagctga aatacctggt ggatcacgaa tgggtccgcc gcgccgacga cgccctgtgg
    11341 cgtcgcacaa aacaaggcat gtggctaaat gcggatcaac aatctcgtgt gagtcagtgg
    11401 ctggtggagt atacgcagca gaagttgtcg ctggcgtcgt aaggatgatg cccggttgtg
    11461 aagtgcaacc gggctatttg gattccttag actattcatt gtaaataatt gaataacggc
    11521 ccctgagcta aaatcgccaa acttaagcac gttacccaat cgccccactt cttcctctgg
    11581 tttctatttt ttctgcacca atggcaataa tatcccaatg caaaaccaag ctgtgcacta
    11641 atcgggccga caaaatcagt ccaaggctgc ggccaaaaag gggaaaaatt caggggaagc
    11701 cagacaaacg tcagaaagaa atttcggata ttttccagca acgctgattt ctctggagaa
    11761 atataaagaa tgaaattcga gagaattaac cagaaataga ccggcgaaaa gaccagccag
    11821 caatgcagtc tgttttttaa ttcaggaggc gttctgcggg agaattgcca accgatgcca
    11881 gccattacac tgaaaaaaag tgcatattca accattaact caagttgtag ttcctgaaga
    11941 gaaatacgca caaccaggag cggaaaggag aatattattt ctgccagaaa agttaacgtg
    12001 aatataagta cggctaagac aataaccgtc ggccatacca ataatggcac gataacaaaa
    12061 ccgagaatta cagggggaat ataaagccgg aatttaatca tttttatttt cctggtgatt
    12121 cggatgatgc gtcatacatc gcgcataaac actcatcaat tatccagtcg tctacccaga
    12181 cctgatgctg tgtgccctgt tctggctgct ttggtgggtt ctcaatcgcc tgccgcgcgc
    12241 gttctttcaa gatggattga ttgttgaagg cccaggcatt gatttgggtg gtaatgacgt
    12301 tatcgatatc gtcctggtta tctctgatat aatcctcaca atatctgttc aataaacagg
    12361 caagagaaac ggtcgaccaa aagccgggaa ctgaattaat ggcatccagc aatggcgcaa
    12421 gagcgatatc ggcacggaaa aacatttcca gcatatactg taattcgaca gcttcatcgc
    12481 cggataccca atcagtcata tactggcagg caaatcgttg caaaattttc cattcatcct
    12541 cacgccaatt ttctttactg gcaaggttta tacgccgtag agaaaatact tcgtgaatat
    12601 tgtcaaactg cccctgtgca acaaattcca gtaaacgcgg cagaaaatag cggacctcat
    12661 cgctattttg cggatcaggc cctgggctac tattccatgc attgattaag gaatacgaga
    12721 tcgctcgtaa cgatgtactc cgtaatacct tttgttcctg ctgactcagg caatattcac
    12781 aacaaaccac aaatttctgc ggcaccggat aacgacgaaa aatatgataa atctgctcaa
    12841 ttaatgcctg catggcgggg ctgggttgca taattacccc ctcgcccgct gatcgcgtgt
    12901 aaacggttga taaacaaaac gagcattcgc cacgttcagg cgaataatgg cactacgaga
    12961 gagggaaaag ctggggcgaa tgcaactatt gcagtctcga cagctttctt cagcgcaatt
    13021 ttccactgtg taataacgag cgtagagttt cagttccgct tgcttatcac gaaatccgcg
    13081 taaactgtta tcgttatgaa atttccaggc atctttaaag acctgaaaaa tgtagatatc
    13141 ctcaatccag gactcacatt ttttaagcgg gcgctttgct atgatcaccc gctcatccgg
    13201 gtttagcatc gccgtaaatt gaacggtgag gtcatttgtc gtgttcagat cacattgaac
    13261 atctggtaca tcactgatat aggggtcaaa tccaaagtca gcaccacagg cacaaagacg
    13321 gctaatggtg gcggtatcga gggaaaatcc cggccataca ttgctggacg ttaagccaat
    13381 atttaatgtg acattatatc gaagcaatcc ctcaagaccg tcagcaccga cgaacttttc
    13441 cccttcatcc agggcctggt taatgagttg ctgaacaatg ccttcatccc agtatgccga
    13501 tttgcagttg gcaatcgtaa tatccacgcc tgtgaatgga tcagtaccga caaggcttgc
    13561 aaataatgta ttaactaatt ccatttttat tatccgcctt aatgcaacgt tttatacatc
    13621 ggcacataac ctacatcttc caccaggctc tgcccctgcg gcgttaaaaa ccactccacc
    13681 aatttttgtg tttctgacgt catattttcc cgcgttacca taaaggcatc gacgatatag
    13741 ggatatttgc cgttgcgaat gttttcggca gtcggtgcaa taccgttaat cgctagcagt
    13801 tttatatttt tatcggcgtt catttgtgtt gcgtaataac ggaaggtgta acctattgcg
    13861 ttgtttgtat tacggtattc ggcaacgact ttaatcattc cctccatcat acttgccact
    13921 tctgtttcct gcggcgagat catgcggaca tttttcatga cctgggattg catcaccgtc
    13981 tggctaccag agtcttccgg gcgttgccag gtctggatct cctgatcgtt accgccaacc
    14041 gtgcgccagt tggtaattgc accactgaag atgtcacgca cttgttgttc ggtcagggaa
    14101 ttaaccgggt tatctacatt gacgatgaaa acaaacgctt cacgggcaaa aggcgtgtaa
    14161 atcaaagtga cgcccgattc ctccgcgcgt tttttctgcc caccggaagg ttgagccaca
    14221 aagataatat cggcattacc tttaacaatc ttgttatatg cttcgggagt acgagagttc
    14281 gccagatatt cccattcgtg aaaatcttcc ggcaaaacgc ttagtgcata aaaggcagag
    14341 gcataaatgg ggtacgccgc cgttgcgcca tcaagtcgcg gccagttttg cgtgaactga
    14401 atttgcggtt taccacgcag tggtgtcagt tgattattga gtttatcggg tcgccaggcc
    14461 caggtatcaa tattttcacg cacaccgacg ccgggattaa gctgtttata taaataagcc
    14521 tggtaaccgg cgacaaaacc aagcatgaga aggataaaca agagagaatt acggcaacgt
    14581 aacccggacg tgcccgttaa aggatatctg gtcggccagc cgtaccccag cgaaaaactg
    14641 atatgcgcgc caacaggtat aatgactaac caccagtact caccaccgac taatgaggct
    14701 aacaaaacgc ccagccaggg gagcataaga tcagccaacc aacccgctcc catctggcga
    14761 aattgataat ctgccagatg cagcgcgatt atccagaccc ccagggttaa taatgcgggt
    14821 actaaaaaaa gacattgtcg ccagacggct atttctcttt tttctggtgc actatcgtcc
    14881 gcccacactc gccccgagaa aaatagcatt aatgcgccca gcgccagcat aaaaatgttg
    14941 agcagaaggt aaattgttgg cgtgataaag atattacaaa cttcaataat accggcaagt
    15001 ccgacaccca gcatggcaat aaccgccgcc aaaaactgcg ccagaatggg gatgccgaaa
    15061 aaagtcatta ccagcgaggt caaaatccat tttctgtttt gcattattct ttccattctt
    15121 tctgaatggc gaaattatac tccccgagtc cccttgcccc ttctggacac ttttccgaaa
    15181 tgatggcgaa aaaaacggga ccctttggcc ccgttctatt tattagtgaa attacaatct
    15241 caccggatca atatgccaga tatgatcggc gtactctttg atagtacggt cagaagagaa
    15301 gtagcccata ttggcaatgt tcagcatcgc ttttgcggtc cactcttcct gaagctcgta
    15361 gagttcgtcg actttatcct gacaatcgac atagctgcga taatccgcca gaacctgata
    15421 gtgatcgccg aagttgatca gcgaatcaac cagatcgcga tagcgacccg gatcttccgg
    15481 actgaataca ccgctgccga tttgcgtcag cacctgatgc agctcctcat ctttctcgta
    15541 gtattcacgc ggtttgtagc cctgacgacg cagttcttcc acttcttccg cagtgttacc
    15601 aaagataaag atattgtcag caccgacatg atccagcatc tcgacattcg caccgtccaa
    15661 cgtaccgata gtaagcgcac cgttaagcgc aaacttcatg ttactggtgc cggaagcttc
    15721 cgtccccgcc agcgaaatct gttcagacag atcggccgcc ggaatgatca actgcgccag
    15781 gctaacgctg tagttcggga tgaacacgac tttcagcttg tcgccaatct gcggatcgtt
    15841 gttgatcact ttcgctacgt cattgatcaa atgaataatg tgcttcgcca tgtaataggc
    15901 cgaagccgcc ttaccgccaa aaatattcac gcgcggtacc cacttcgcat ccgggtcggc
    15961 cttgatgcgg ttatagcggg taatcacatg caacacattc atcaattgac gtttgtattc
    16021 gtgaatacgt ttgatttgta catcgaacaa cgcctttgga ttcaccacca cattcagctg
    16081 ctgggcgata tactctgcca gacgcttttt gttctccagc ttcgcctgat gcacagcgtg
    16141 attaaccatt gggaaatcac agtgttgttg cagctcatta agcaggctaa ggtcggtgcg
    16201 ccagttacgg cccaggtgtt cgtccagcac ggctgaaagc gatgggttcg ctaccgctag
    16261 ccagcgacgc ggcgtcacac cgttggtgac gttggtgaaa cgacccggga agattttcgc
    16321 aaagtcggca aacaacgatt gcaccatcag attagagtgc agttccgata caccgttaac
    16381 tttgtggctc acaacaaccg ccagccaggc catacgcaca cgacgaccgt tggattcatc
    16441 aatgatcgac gcccgtccca gcagatcggt atcgttcgga tactgttcct gcaaggtttt
    16501 caggaaatag tcgttgattt caaagatgat ctgcaggtga cgcggcagaa ttttacccag
    16561 catatcaacc ggccaggttt ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa
    16621 gacctgacaa cacacctcaa acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag
    16681 acgcatcatc tcaggaatcg acagtaccgg atgggtatca ttgagatgaa tcgcgatttt
    16741 atccgccagg ttatcgtagg ttttatgcaa ctgataatgg cggcttaaaa tgtcctgaat
    16801 ggtcgaggaa accaggaagt attcctgacg caggcgcagc tcacgcccgg agtaggtgga
    16861 gtcatccgga tacagtacgc gagatacgtt ctcggagtgg tttttatctt ccactgccgc
    16921 gaagtagtca ccctggttga atttaccgag gttaatttcg ctactggctt gcgcactcca
    16981 caaacgcaac gtgttggtcg cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac
    17041 tcccagaatc tcttcggttt caatccaacg cgttttttta ccttcctgct gaatgcgacc
    17101 accaaaacgg actttatagc gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc
    17161 cagccagtag tctggcgact ctttctggct accgttaacg atgttctgct tgaacatacc
    17221 gtagtcatag cggatgccgt aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag
    17281 gaagcaagcc gccagacgtc ccaggccacc gttaccgagg cctgggtcat tttcttcatc
    17341 aatcagctct tcgagattta accccatcgc ttccagtgcg ccctgtacat cttcgtaaat
    17401 tcctagcgac aacatggcgt tggagagcgt acggccaatc aaaaactcca tcgacaggta
    17461 gtaaacctga cgagtttctt gcgacaactg ggcacggttt gaacgtaacc agcgctccac
    17521 gagacgatcg cgcacagcaa ataacgttgc gttcagccat tcatgtttat tggcgacgac
    17581 cgggtccttt ccaatcgtaa acatcagctt gtaagcgata gagtgcttaa gagcttctac
    17641 gctaagcgtg ggcgatgaat atgtaaacgg agcattcata taggcgtttc ctgaaaacta
    17701 tttcaagcga tagtaaagct cacggtacga cttcgccgcg acctgccagc taaaatccat
    17761 tgccatagcc tgacgttgca caaaccgcca cagtgaagga cgggaccaca gtacaaaagc
    17821 acgtcgaata gcccgtaaca gcgaccaggc attactatct tcaaagacaa acccactggc
    17881 gacgccatct gcaaggttct caagagaaca gtcagaaacc gtatcagcaa gcccaccggt
    17941 gcgccgcact aacggcagcg taccgtactt caatccataa agttgcgtta agccgcacgg
    18001 ttcaaaacgg ctgggcacca gaatgacatc cgcgccgccc ataatgcgat gcgaaaatgc
    18061 ttcgtgatag ccaatctgaa cgcccacctg gccggggtat tccgctgccg ccgcaaggaa
    18121 accttcctgc agcaccggat cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag
    18181 aaggcccggt aaggcttcca gcaccaggtc gagacctttc tggctggtca gacggctcac
    18241 cactgcaaaa agcggcactt tatcatcaac cttaagcccc attgcgattt gtaactggcg
    18301 cttattttcc gctttatctt ccaacgtatc gcgggtgtag cgcgaggcca acagtaagtc
    18361 cgtctctgga ctccagattt tctcgtccac gccgttcagt acgccggaaa gacgcccttc
    18421 acggtgacgc tgttgcaaca gaccttccat accgtaggca aactgcggtt cggtgatctc
    18481 gcgagcgtag gttggactga ccgccgtaat gtgatcggca tagtacagac cggccttcag
    18541 gaaagagatt tgtccgttga attccagccc atgaatatta aagaatgacc atggcaattg
    18601 gatgtcattc atatgatgtg cataaaacat gccttgatag gccaggttgt gcacagtaaa
    18661 caccgacttc gccggacgcc cgcgcgccgc cagatacgca ggcgcaaggc ctgcatgcca
    18721 gtcgtgcgca tgcaccacat caggacgcca gaatgggtca agcccgctgg ccatttctgc
    18781 cccaacccac cccagcagcg caaaacgcaa tacgttgtcg gtataggcaa ataagttggt
    18841 atcgtgatac gggcttcccg gacgatcata gagatgcggc gcgtcaatca ggtatatgcc
    18901 aaccccgttg taatgaccga acaacagcgt gatatgtccg gcgaaggtat cacgacggga
    18961 tactacctgc gcatcggtaa cgccacggcg aatatcggga aatgcaggca acagtacgcg
    19021 agcgtcaacg ccgtctgcga tttgtgctgc gggtaatgcc ccaataacat cagccagacc
    19081 gccggtttta agcagcggga acatctctga acatacatgt aaaacctgca ttatcgctcc
    19141 tgtttatgcc ctaacttccg tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa
    19201 cgatagaaac gacgtgcatc ttcctctgcg ttttcaccaa tcaccatgcc ttccggaata
    19261 acacaagcac gatcgatgac gcagcggcgc agacggcacg agcgacctac ccatacttcc
    19321 ggtaacaata cggcggaatc aatgttgcag aatgaattca cgcgaacgcg cgagaacaga
    19381 acggactgca ccaccaccga accggagatc acacagccgc cggataccag tgagttaagg
    19441 gtcatcccgt ggctaccgga gcgatcctgc acgaatttcg ctggcggtaa tgattcattg
    19501 taggtgcgaa ttggccaatt gcgatcgtac atatccagtt ccggcaccac agaggccaga
    19561 tcgaggttcg ctttccagta agcttccagc gtacccacat cgcgccagta cggctcggca
    19621 tccgggtcgg attgtacgca agagagcggg aacgggtgcg cataggccag accggcttcg
    19681 gtgatcttgg gaatcaaatc tttgccaaag tcgtggctgg agttctcatc acgatcgtct
    19741 tcttccagca gttcatacag atagtcggcg tcaaagacgt agatacccat actcgccaga
    19801 gatttgctcg gatcgttcgg cattgacggt gggttagcag gtttttcaac gaattcgata
    19861 attttatcgt tctcatcaac cgccataacg ccaaatgcgg aggcttcttc aatcggtact
    19921 ggcatacaag caacggtgca acgtgcgcct ttttcgacgt gatcgataag catacgcgag
    19981 tagtcttgct tgtagatatg gtcgcccgcc aggatcacca cgtattccgc tttataacgg
    20041 cggataatgt cgaggttttg ggtgaccgca tctgcggtgc cgcgatacca gttttcccct
    20101 ttcattctct gctgtgctgg cagcagatcg acaaactcgt tcatttcttc attgaagaat
    20161 gaccagccgc gctgaatgtg ctgcaccaga gtgtgggact ggtactgggt gatcacgccc
    20221 atacgacgga tcccggagtt gatgcagtta gacagcgcaa agtcgataat gcggaactta
    20281 ccgccgaagt gtacggccgg ttttgctcgc ttattggtta aatccttcag gcgggtacca
    20341 cgtcctcccg ccagtatcag ggcaacagat ttcaatggca gctggcgcgc caacattaag
    20401 tgatcgttct tctctaaact aaccatgact aactcctttt ttatcatctc tggaacacac
    20461 acaatccgtg tgcaggtccc tgccagacag ccgtaatcac tgggttatcc tctccagcga
    20521 atgggggaat ggcgtgccac tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg
    20581 cgttaattgc gatcaaaaag cgatccgaga gcagaatttg cagctgtttc ggcccgtttt
    20641 gccactcatc cgtgcttaaa ggttgagcat atcgatttag ccaacggaca ttgccgtcgc
    20701 cttcttccca ccagcgattc tccaccaaag cgggaatgcg cttgcgcaga tggattaacg
    20761 cggcggtaaa tgcggttaaa ccactgcttg cctgcgacca gtccaaccag gttaattggt
    20821 tatcctgaca gtaggcattg ttattgccat gctggctgtg accatgttcg tcaccggcca
    20881 gtaacatcgg cgtaccctgg gagagcaaca acgttgttaa cagggcgtga atgctgtcgc
    20941 gccgccgttc aaccaggtca agagaaccgc ctaacccttc tttaccatga ttgttactgt
    21001 aattgttgtt ggtcccgtcg cgattttctt ctccgtttgc ttcattgtgt ttatggttga
    21061 agcaaacgca gtcgcgaagc gtaaaaccgt catgcgcggt gacgagatta atcgcggcac
    21121 tcggcagacg accattacgt ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg
    21181 cccccagagg caaatcataa tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt
    21241 tccactcggc aaacagcggc gggaaatttc ccacctgata accaccagga gcgatatccc
    21301 acggttcagc aattaacttc acctgcgaga gcaccgggca gttctggata gcggtaaaca
    21361 acggcgcatc ctgacggaac tctggcgtac ggcccatgac tgccgccaga tcaaagcgga
    21421 aaccatcgac gtggcaggtt tctacccaat aacgcaggca ggcgctggca taatccacca
    21481 ccgccggatg actcaaattg agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt
    21541 cttctcttat ccaataatag ctacggttat cgatcccacg cagcgagaat aacgggccgt
    21601 cgaggtccag ttccgcacta tggttgagca cgatatcaag aatgacttcg atacccgctt
    21661 tatgcagtgc tttgattgca tcgcgaaact catccagcgc cgtttctggc gagcaggcat
    21721 acgccggatg cagcgcaaac atcgccaccg ggttgtaacc ccagtagtta cttagcccca
    21781 tgcgttgcag acgtggttca ctggcaaact gcgccactgg cagcagttcc agcgcggtaa
    21841 tgcccaattg tttcaaatag ttgatcatca ccggatgccc gagggcttta taagtgccac
    21901 ggatctcgac cgggatctcc gggtgcaagt acgttaatcc tttgacatgg gcttcataaa
    21961 tgatggtgct gccccacggc gtgcgcggcg gggcatcatc ttcccagtca tagtgatcaa
    22021 ccaccactac gcatttcggc gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac
    22081 cggcgtgcag cagcgggtta tctttaaact ccccctcaat ttgccgcgcg caaggatcaa
    22141 tcaacaactt cgccgggtta aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc
    22201 gataaccata acgcaaaccc gggcgcgcat ccggcagata accgtgccaa atgtcgccac
    22261 tgtgccctgg caagtcatag cgatgttcct ggccattggc gtcaaagaca cacagttcta
    22321 cccgctcggc atgagcggag aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc
    22381 cgaggggagc gggtttgcca atggcgagtt gtgtcattct gcctcccgaa ccagccagat
    22441 agtggccagc ggtggtagcg tcaggcttag tgaatgctga cgaccgtggc tggcaatctc
    22501 atcgctgtgt accgtgccgc cattgcctgc attactgccg tgatagtgca tggaatcggt
    22561 attgaggatt tcacgccatt tgcccggctg gtttatgccg aagcgataat catgacgcgg
    22621 caccggcgta aagttactgg caacgatgat ttcgttaccc tctttatcgc gacgcacaaa
    22681 gatcagcacc gagcgttctt tgtcatccac caccagccat tcaaagccgt acgggtcaaa
    22741 atccagttca tgcattgctt tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg
    22801 ctggacaccg tggtgccagt tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc
    22861 atggttccac tcgcggccct gggcaaattc gttacccatg aacagtagtt tcttgcccgg
    22921 gaatgcccac atccagccat agtaggcgcg caggttcgcg aatttctgcc atgcgtcgcc
    22981 cggcatgcgg tcgagaatcg attttttacc gtggaccact tcatcatgcg acaacggcag
    23041 gacgaagttt tcagtgtagt tgtagagaat cccgaaggtc agtttatcgt gatgatactg
    23101 acgataaacc gggtcgagct tcatgtagtc cagggtgtca tgcatccagc cgaggttcca
    23161 cttgtaccag aagcccagac cgcccatatc ctgcggacga gaaacgccag ggaaatcggt
    23221 agactcctca gccattgtca ccgcaccgga aacctgctca ccaagaatac ggttggtatt
    23281 acgcaagaat tcaatcgctt caagattctc gcgcccgcca aattcgttcg ggatccactc
    23341 cccctcttta cggctgtagt cgcgataaat cattgacgcc accgcatcga cgcgcagcgc
    23401 atcaatacca aaacgttcaa tccagtaaag cgcgttaccg acgaggaagt tactgacttc
    23461 acggcgacca tagttgtaga tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc
    23521 gctgtgttca tacaagttcg tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa
    23581 gtggcctggc acccagtcga gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa
    23641 ataacggaag tcgtcgcgag taccaaaacg gcgggttggt gcatacaggc cggttggctg
    23701 ataaccccaa ctgccatcga agggatgctc gttaatgggc agtagttcga ggtgggtaaa
    23761 gcccatccat ttagcataag gcaccagttg atcggccagc tcgcggtagc tcaaccagaa
    23821 attgttgtcg gtgtggcgac gccaggaacc caggtgaact tcataaatag agattggcgc
    23881 atcaaactga ttcgcttttt tgcgctcttc agtctgtaca accttttccg gcagcccgca
    23941 aataagagac gcggtttccg ggcgcatttg cgcttcgaag gcataagggt cggacttcag
    24001 acgcaagttg ccattggcat caatcatctc gtatttatag agctgaccgt tatgcgccca
    24061 agggataaac agttcccaga tgccgctctc tttacgcagg cgcatcgggt gacggcgacc
    24121 gtcccagtag ttgaattgcc caaccaccga gacccgacgg gcgtttggag cccagacaga
    24181 gaaacgcgta cctgtgacgc catccatagt atctgcatgc gcgcctaagg tttcatacgg
    24241 gcgcaggtga gtaccttcag ataatagcca ggcatccatt tcctggatta gcggaccaaa
    24301 acggtaagga tcatcaatca ggttttgctg accatgccag acaacagcca actgatagcg
    24361 gaaaaaattc ttacgtcgcg gaatgacgcc gctaaagaat ccccgtgagt cgagacactc
    24421 cagttttgcg agtttgcgcc cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg
    24481 taaaagggca cggacttcca gtcccgcggt ggttttatgc attcccagta cggaaaaagg
    24541 atccgcaaaa tggcctgcaa ttagcgcgtt aatcacgtct ctatcgatac gatcggacat
    24601 gcttgtcttc ctgttttatt gtgtcacccc atccaattgg atttttgact tctggttgtg
    24661 acattttttt gacctgaacg gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt
    24721 caggtaaggc tgtgaatact catgtattca gccaccctta aagaatagcc aatgctctat
    24781 ttaactcccg gtaaatcatg aaacatctgc gcttactcct gtattacgca ctaacagggg
    24841 cggcatcgcg ccccagattt aatgaataaa gattacgcca gttgacgaag catccgacgc
    24901 agcggctccg cggcccccca cagcagctgg tcgcccacgg taaaggctga caggaactct
    24961 ggtcccatat tcagcttacg caggcggcct accggcgtgg tcagcgtgcc ggtaacggca
    25021 gctggggtta gctcacgcat agtgatttcc cgatcgttcg gaacgacttt cgcccacgga
    25081 ttgtgcgcag ccagcagttc ttccacggtc ggaatagaca catctttttt caatttaata
    25141 gtgaatgcct ggctgtggca gcgcaatgcc ccgacacgca cacataaacc atctaccgga
    25201 attacggaag atgtgttgag gatcttgttg gtttccgcct gccctttcca ctcttcgcgg
    25261 ctctgaccgt tatcgagctg tttgtcgatc cacggaatca ggctacccgc cagcggcacg
    25321 ccaaagttat ccaccggcag ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata
    25381 tcgagaatag cagaggacgg ggtcgcgagt tcatctgcca catggccata cagatggccc
    25441 atctgggtta ataactcacg catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt
    25501 gcaacggaca cccaatcaac aagatcattg gcgaataaac cacccaacga catcaacatc
    25561 aggcttacgg tacagttacc gccaacaaaa gtcctgatgc cattatttaa tccgtcggta
    25621 atgacgtcct gattgacggg gtcaagaatg atgatggcgt catctttcat gcgcagagac
    25681 gatgctgcgt caatccagta accttgccat ccgctttcac gaagctttgg atagatttcg
    25741 ttggtataat cgccgccctg acaggtcaca atgatatcga gggcctttag cgcctccaga
    25801 tcaaaggcat cctgaagtgt gccagtggtt ccgccaaaag acggcgcagc ctggccaagc
    25861 tgagaagtag aaaagaagac agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt
    25921 tgcatgagaa cggagccgac cataccgcgc cagccgataa aaccaacatt tttcataagc
    25981 gtttttttcc tgcaaagatg tgtgctgtat aaatgtgccg gtctcctctt ggcacatctt
    26041 tcaccataca aaaagcagcc aaagtcgcaa gtgaaattaa tcaatgatag cgaagccatc
    26101 agtaatgcga cttatcctgc tttgttagca cgcagaaagt ccgcggcaat tatcagggaa
    26161 tttgagttat gaatgaaatc atttctgcag cagttttatt gatcctgatt atggatccgc
    26221 tcggaaacct acctattttc atgtccgtac tgaaacatac tgaaccgaaa agacggcggg
    26281 caatcatggt gcgagagttg cttattgctc tcctggtgat gctggtgttc ctgtttgcgg
    26341 gtgagaaaat tctggcattt ctcagcctgc gagcagaaac cgtctccatt tctggcggca
    26401 tcattctgtt tctaattgcc attaagatga ttttccccag cgcttcagga aatagcagcg
    26461 ggcttccggc aggtgaagag ccatttatcg tgccgttggc aattccgtta gtcgccgggc
    26521 cgactattct cgccacgctg atgttgttgt ctcatcagta cccgaatcag atggggcatc
    26581 tggtgattgc tctgctgctg gcctggggcg gcacctttgt catcctgcta cagtcttcgc
    26641 tatttttacg tctgctgggc gagaaagggg tgaacgcact tgaacgcctg atgggattga
    26701 ttctggtgat gatggcaacc cagatgttcc tcgacggcat tcgaatgtgg atgaaggggt
    26761 aacgaggaga gtttttcgaa aagaacccgg tagcgtcaca actaccgggc gaacaaactt
    26821 aactcaacag ctggaacgca atcatcccaa cgatggcacc gacagtgccg aggatggttt
    26881 ccatcatggt ccaggttttc agcgtttcgg cttcggtcgc gccggtaaat ttaccgaaca
    26941 accagaaacc ggcgtcgtta acgtggctga caacaatcga accaccagcg atacaaatcg
    27001 acagcgccgc catttgcgca ccggagtagt tcagttgttc aataaccggc atcaccagtc
    27061 ctaccgccgt taaacaggct acggtggcag aaccctgaat gatgcgcact gcagctgcca
    27121 gcacgaagca ggtgatagca atcggcaggc ccatgccggt taacgcttcg cccagtgccg
    27181 gacctacgcc agagtcaacc agcacctgtt tgaacacgcc gcctgcacca atcaccagca
    27241 gaatgatccc cgccggttgc agcgcgtgac cgcaaatctc catcactttg tcttttggca
    27301 tgccctgacg cattgccagg ccataaatcg ctaccagaca agcaaccaga atcgcggtaa
    27361 acggatgacc aataaactcg aaccattcgt aagcggtaga gccttctggc acaaaacgcg
    27421 cggcaatggt tttcagcccc accagcacca gcggcaacag gatcagcgac aggctgaatc
    27481 cgaaagatgg cattttgcct tcgccgagat gcggttcgct gatgtcgtca ggaatatgca
    27541 actccacgta gcggctgatg aaattacccc acagcggccc ggcaataatc attcccggaa
    27601 ttgccgcaca caggccaatc aggatcatcc agccaaaatc ggcgttcatc tgcgatgcca
    27661 gcagcattgg cgctggccct ggcaccagga acgccgccgc tgccgccacg cctgcgaata
    27721 atgggattac cagcttcacc aggttcgtac cggtgtggcg cgccattgag aaagcaacgc
    27781 taatcagcag aactatcgcc acttcaaaga acagcggtag cgcacagacc aaccccgcaa
    27841 ggccgatggc aaaatgcgcg cggctgtgac cgaaggattt gagcattttg acggcaatct
    27901 gatcgactgc gccggtttca tgtaagatct tgccaaacat agctcccagg gcgacaacca
    27961 ccgccaggaa gccgagggtg cctcccatcc ctttttccat cgtcgctgcg attttatcga
    28021 gcggcatacc ggaaaaaagg ccagccccca tggacaccac catcaaagcc aggaaagcgt
    28081 gcatacgcgc cttcatgact aaaaacagca gcagtaaaac agaccctact gctgttaaaa
    28141 caagcgttaa tgtagtcact acttatttgc cttttttaat aacctcaatg gtgcttgcca
    28201 caacaccttc cagcggttga tcgatatcca ccaccagtac atcggtttcg tccgcacccg
    28261 gctcctgcag cgtttcaaac tgcgtcacca acatttgggt tttaaagaaa tggcctttgc
    28321 gcgctttcaa gcgactttca atcacatcaa aatcgccttt caggtagatg aaagagagat
    28381 tcggattacc ttcacgcagc aagtcgcgat agtgcttttt cagtgcagaa cagacgatca
    28441 gcgacacttt attggtgcgc tgcatagcaa acgcggcgtc gttcagcgcc tgcagccacg
    28501 gtttgcgatc gtcatcattc agcggttcgc cagacgccat tttttcgata ttgcgccgtg
    28561 gatggaggaa atcgccatca agaaacgcgg catgaagttg atgcgccact tcactggcga
    28621 ccgcagattt gccgctgccc gatacgccca tcaagacgta aatgtggtga tcatggttag
    28681 tcgtgctcaa agtggtgccc ccacaataca agaattaaaa atgttacggg taactgttat
    28741 cggtaacatt gtccagccgg acaatatcag aagcaatatc catccgtgcc ttaagtgtat
    28801 aagtgtgagc tacttcaaat ttgtgggctt aaatagatcc gcccggtgac aaggtgaaac
    28861 ctaaatctaa cattttcggt gtcacagatt cgccacgaat acgcgccagc aggcgttcag
    28921 cgccaatact gcccatccgc tcacgcggcg tcagcacgct cgccagtcgt ggctccatca
    28981 cctgaccaat gtcatgaccg tggaaaccgg caatcgccat atcgtcagga acttttaacc
    29041 ccagacgctg acattcaaac gccgcgccga ccgccagatc atcattagta cagaacacgc
    29101 catccagctg cggatattcc cgccgcgcct ggcgaatcag ttcaataccg gaagagtaag
    29161 aagaagattg ctcaaccatc acgctatacg gcaccaggcc tgcatccagc atcgcctgtt
    29221 cgtatccctt ctgtttgatg atagtacgtt cgtcgagacg tgcgccgaga taggcaatgt
    29281 ggcgatgccc gcgcgcaata atggcggtag tcatctggcg tgccgcttca aagttatcaa
    29341 aaccgacggc gatatcgagg cacggcgact ggctgtccat caactccacc accgggatcc
    29401 ccgccacttc aatcatcttt aaggtgcgcg gcgtgtgggt acgttcggtg aggatcaggc
    29461 cgtcgatatt ccaggacagc atggattcga gacgttcttg ctccatttcc ggtttataac
    29521 cgtagtgcgc cagcatcgtc tgataaccgt gcgcgtcggt gacgctttcg attccgcgta
    29581 atacttccgc gaaaacctgg ttggtgagag aaggtaacag gacgccaatc gcccggctgg
    29641 tagcgttaga gaggatatcg ggcgcacgat tgggaatata gcccagttca tcaagagcgg
    29701 cggcaatctt gccgcgtaga gcgacggaaa cctgctccgg gttgcgtaaa aaacggctga
    29761 ccgtcatttt ggtcacgcct acacggtcag ccacatcctg aagtacgggt cttttctttt
    29821 tcatcgtcct gaaggtacaa aagagataga ttctcttagt ttaacacgga cgcatcagaa
    29881 ccttcccggg gaaaacagtg cttgtttaca attattagat atacactaaa tcaattgcat
    29941 cgcattgtgc taacgacgcg gcaaaacccc ggaagcgtgc atcagcacgc gaccggagcg
    30001 agcgaggaag cctgcaaaaa ggcaggcttc aaagatgccg ttgagtttta aaccggcggc
    30061 agatcgaaca gtaacacttc gctgtcgcta tccgcatgga tggagattgc ctgctcatcc
    30121 cagattgcca gaccatcgct ggtcgaggct ttcacgccgt taatggtgac attgcctttc
    30181 accacctgga tccagacgcg gcgttcagcg gcaatctgat gcaccgactg ctcatctttc
    30241 agcaacgccc aacgatacag ttccatatcc tgatgcactt tcagcgaacc atctcgcgca
    30301 tccggcgaga gcaccagctg tttgccctgc acggcatcga agcgacgctg ttcataacgc
    30361 ggcgtaatgc cattttcttc aggcatgatc cagatctgat acagatgcag acgctcggtg
    30421 ctgtttgggt tgtactctga gtgacgaata cccgtacccg cactcataat ctggaactca
    30481 cccgccggaa cctgctcttt attgcccatg ctgtcctgat gctcaacagt accttccagc
    30541 acgtaggtca aaatttccat atctttatgc gggtgagtgc cgaagccctg ccctgcttca
    30601 atcacgtcgt cgttaatcac gcgcagcgcg gagaagccca taaagttcgg atcgtaatag
    30661 ttggcaaaag agaaagtatg ccaggaatcc agccagccat gatttgcatg accacgttca
    30721 tttgctttgc ggaagtagat catttgtatt cgccccctga atgatttcga tgggcttagt
    30781 gtggacccga tctgcctggg atgatagagg gtgaaaattg acccctctgt tcaaaaaaat
    30841 tgaactattc gaggggccat caaacttact tagcgagagt tactgtggag ggagaggctt
    30901 gctcaaatcc gcgttcaagg atttccagat tggtaagaac ttcagattcc ttgacgtaat
    30961 ttggcgcacc gtgggtgatg gtttgataca acgcatcata aacgcgcccg taatcgccca
    31021 tctccggctt catctcttct ctgaccgtca cgccctcgtc attgacatac tccagcacac
    31081 cgaccgaatc atccgctgcg aatcccggtt cgcccggcat aatattagcc ttcaggctgg
    31141 tttcctgctg gtcgataccg tatttaataa acgaaccttt cttaccgtga acgataaatt
    31201 tcggataatc gattttcacc agatggctgg ttttgacgat ggctttcagg tcgccataaa
    31261 acagttgcgc ttcaaaggtg tcgtcaggat tggctttatt acgcaggctg cggatgtcat
    31321 aagcgacgtg atccgggcga ccgaacagag aaataatctg gtccatcgta tgcacaccaa
    31381 ggccataaaa cgcgccatcc tgcggcagcc caggtttggt ttctgccacc gggcggtaat
    31441 agtcaaaatg gctttccact tcaacaatct ctcccaactt gccactttca atcgcttttt
    31501 tcgctgtcag gaagcaggag tcaaagcgac gattctgata cggcgtgacg gtcagccctt
    31561 tgcttttcgc caacgcaaac agctccttcg cctgggcaag cgtcggggtg aacggttttt
    31621 cgaccagcac atttttcccg gcttccagcg cgcgtttcgc gtactcgaaa tggctgtccg
    31681 cgtgggtgca gacaacaacc agcttaacat cgggatcgtt tagtacttcg tcgagatcgc
    31741 tggtcaaatg gatatgggaa taaatgggag cctgttcttc cggcttcgca tggcgacgaa
    31801 aaatatgcgc gacatgccag ctatccttgc ggttaagtac atacggcaga tggtaacggg
    31861 tggtgctttt gccgaagcca ataaaggcgc agttgatgac catgatttcg tcctttttaa
    31921 ggtggttatt tacaccttag cgcaaagcgg acgtggttcc tactggagtg cgcataacga
    31981 acacaagcac tcccgtggat aaattgagaa agaaagatca aaaaaaaagc cagcacccgg
    32041 ctggctaagt aatactggaa gcaatgtgag caatgtcgtg ctttcaggtt ctccgcgagg
    32101 gtcttcctga tcgcgagaca ataataatca ttctcattcg cacttgtcca acactttttg
    32161 caaaaaaatg catttgactc gtatttgaaa gtcaatgatg ttgaaaggga catttacccc
    32221 aaagaggaca aaggaatgag tgagatagta atacgccacg cagaaacacg ggattacgag
    32281 gccatcaggc agattcacgc ccagccggag gtgtattaca acacgctaca ggtgcctcat
    32341 ccttccgatc atatgtggca ggagcgactc gctgctcatc ccggcatcaa acagttagtt
    32401 gcctgtattg atggaaccgt cgtgggtcac ctcaccattg acgtgcaaca gcgcccacgc
    32461 cgcagtcatg ttgccgattt tggtatctgt gtcgactctc gttggaagaa ccgcggcgtc
    32521 gccagcgccc tgatgcgaga gatgattgaa atgtgcgaca actggttacg ggtagatcgc
    32581 attgaactaa ccgtgtttgt cgataacgcc ccggcaatta aggtctataa aaaatacggc
    32641 tttgaaattg aagggactgg taagaagtac gcattgcgta atggtgaata tgtcgatgcg
    32701 tattatatgg cgcgggtgaa gtgaaaacac ttaacttatt gtaaatacga atatttgatg
    32761 aaggctttgt tggatatgtt gcgcttgaaa aaaagcacga cttaactacc ccgtcgaaaa
    32821 attcataata attatgatga tatatcagga agcattcgcg aaagcgaacc attaccttga
    32881 tgatgcagat ctccctgtcg tcatcactct gcatggacgg tttagccagg gctggtattt
    32941 ctgtttcgaa tcacaagaat ttcttgaatc tggtgatgag gccgcgcgtt tagccggtaa
    33001 cgcacctttt attattgata aagacagtgg tgaaattcat tctctgggaa cggcaaagcc
    33061 gctggaagaa tatctacatg attatgaaat aaaaaaagct accttcggct tgccctgaca
    33121 atacggccct cttcccacga agagggccgc taaccttaat accccgccgt taaatcatcc
    33181 accgagcgcg ggtcggatgc accgtacaac tcaccgtccg gcccaaccat aatgctttgt
    33241 gtactaccca tcgcctcttt cagcgccact ttctgacctt ttgcttccag cagcttgagc
    33301 gtatccgggc taaacccttt ttcgacacgc agctcgtccg gcaaccactg atggtggaaa
    33361 cgcggcgcat tggtcgcttc ggcgacgttc atgccataat cgatgctatt caccaccatt
    33421 tgcagcactg tagtgatgat ccggctcccg ccagggctac cggtgaccag ccaggtttta
    33481 ccgtctttca ccacaatcgt tggtgacatt gacgacagcg gacgtttatt cggcccgacg
    33541 gcgttggcat caccgcccac cagcccgtaa acgttcggca cgcccggttt agcggagaaa
    33601 tcatccatct gattattaag cagaataccg ctctcgcccg cgacaatacc cgtaccgaag
    33661 gtggtgttca gcgtataggt caccgccacc gcgttaccgt ctttatccac cactgagtaa
    33721 tgggtagttt gattactctc ataaggcgca agcttgccag ggcgaatctc actggacggc
    33781 ttcgctttat tgatatcgat ttgatcggca atagatttgg cataggcttt attggtcaac
    33841 gcctgccacg ggactttcac aaaatccggg tcgccaagat attccgagcg gtcagcgtag
    33901 gcatatttct ccgcttctgc catgatttgc atcgcatcgg cgctgccaaa gccgtatttc
    33961 ttcatatcga agttttccaa aatattgagg atttgtacga tatggatccc gccggaagat
    34021 ggcggtggca tggagtaaac ctgatacccg cgataatcgc cgcttatcgg tgtgcgttcg
    34081 accgctttat acgccgctaa atcttctttg gtgatcaagc caccgttttt ctgcatctcc
    34141 tgggcaatct gttccgctat cgtgcctttg tagaattcgt ccgggccgtt ttcggcaatc
    34201 atctccaggc tctttgccag gttcgcctgc accagcttgt cgcccttttt cagcggttcg
    34261 ccctctttcc agaagatagc tttgctgttt tcgtgattcg gcaacacttc gctaccgtag
    34321 gttttgagat cgtcagccag cgcgtcgtta acgataaaac catcgcgtgc aagtttaaaa
    34381 gcgggctgca cgactttatt cagcggcatg gtgccgtatt tatccaacgc cagcgagaaa
    34441 cctgctaccg taccgggcgt gccagaggcc agatgcgaag tgagtgattt tttgctgtcc
    34501 gggttgcctt gatcatcaag gaacatatcg cgggtcgctt tagcgggtgc catctcacgg
    34561 aaatcaatag cagtggtgtt gccatcttta gtgcggatca gcataaaacc accgccgccc
    34621 agattccccg cctgcggatg cgttaccgcc agcgcgtagc ccaccgccac ggcagcatca
    34681 acggcattcc cgccctcctt gaggatatcc acccccacct gagtggcagt agcatctaca
    34741 gacgccacca tcccctgttt cgcgcgtact ggatgaaaga catcttcctc cacgccataa
    34801 gaaacaggag gcgcggcggc ggcgctaaaa caacttcctg agagcagagc agcaatggcc
    34861 acccggcgta aaaacgtcgg ttttatcatc gttattctcc agttatgtgt ggggcaaccc
    34921 cagctaagcc tggtatataa ctctgaatta atcatcgttt tgccgggaag cgagtaaact
    34981 taaagaatat cttcagagga ggatacgcga tgaaacgact tctgattctt acggcactcc
    35041 tgccgtttgt cggctttgca cagcccatta atactctgaa taaccctaac cagccggggt
    35101 atcagatccc cagtcagcag cggatgcaaa cccaaatgca aacgcagcaa atccagcaaa
    35161 aagggatgct gaatcagcaa ctgaaaacgc aaactcagtt gcaacagcag catttagaaa
    35221 accagataaa caataattct cagcgggtgt tgcagtcaca gccgggggag cgaaatcccg
    35281 cccggcagca aatgctgccc aacaccaacg gcgggatgtt aaacagcaac cgtaatccgg
    35341 atagttcgtt gaatcagcag catatgctgc cggagaggag aaacggcgac atgctgaatc
    35401 agcccagcac gccgcagcct gacattccgt tgaaaactat tgggccgtaa agttcggacc
    35461 aattacatca atcgcatcgg tacagatgca atccacgccc cagcgcagca actctgccgc
    35521 gcgctggggt ttgttgacgg tatacaccag aatccgcagt ccggcgtctt tcaactgcat
    35581 cactcgcgct ttatcgagca acttatgatt gagatgaaga gagacgcagc ccagccgcat
    35641 ggtcagttcg cgccagtcgt cgcgccactc atccagcaat aaaccacgcg gcagttccgg
    35701 tgccgccaat tgtgcggctt ctaaagcatc aatctcaaac gatgacagca gcggcggcgt
    35761 cataccggcc cacagttggc gtgccgccag cgccaccatt ttgcccgtta atggtccagt
    35821 gccagtggtg ggtttgattt cgatattcgc catcatcccg tgttcgcgac agcgttccgc
    35881 cacctgcgaa agcaacggca gcggct
//
LOCUS       DAMZET010000029        33084 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.029, whole genome shotgun sequence.
ACCESSION   DAMZET010000029 DAMZET010000000
VERSION     DAMZET010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.029"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(56..916)
                     /locus_tag="POB34_001540"
     CDS             complement(56..916)
                     /locus_tag="POB34_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDI5918516.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLIIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(913..1692)
                     /locus_tag="POB34_001541"
     CDS             complement(913..1692)
                     /locus_tag="POB34_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDI5918517.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCSRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWRDYLRQRMQP"
     gene            complement(1670..3079)
                     /locus_tag="POB34_001542"
     CDS             complement(1670..3079)
                     /locus_tag="POB34_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5918518.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(3101..4555)
                     /locus_tag="POB34_001543"
     CDS             complement(3101..4555)
                     /locus_tag="POB34_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDI5918519.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(4625..5410)
                     /locus_tag="POB34_001544"
     CDS             complement(4625..5410)
                     /locus_tag="POB34_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI5918520.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAASCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            5729..7006
                     /locus_tag="POB34_001545"
     CDS             5729..7006
                     /locus_tag="POB34_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5918521.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSTSLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            7033..8511
                     /locus_tag="POB34_001546"
     CDS             7033..8511
                     /locus_tag="POB34_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDI5918522.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNYFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFMPVESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
     repeat_region   9150..9666
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=9211..9239
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaactc"
     gene            complement(10006..10677)
                     /gene="queE"
                     /locus_tag="POB34_001547"
     CDS             complement(10006..10677)
                     /gene="queE"
                     /locus_tag="POB34_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDI5918523.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            10816..10956
                     /locus_tag="POB34_001548"
     CDS             10816..10956
                     /locus_tag="POB34_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918524.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNNNESKITDAAVNLFI
                     QI"
     gene            10970..11842
                     /locus_tag="POB34_001549"
     CDS             10970..11842
                     /locus_tag="POB34_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HDI5918525.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIKVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSDSGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(11902..13200)
                     /gene="eno"
                     /locus_tag="POB34_001550"
     CDS             complement(11902..13200)
                     /gene="eno"
                     /locus_tag="POB34_001550"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDI5918526.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(13288..14925)
                     /gene="pyrG"
                     /locus_tag="POB34_001551"
     CDS             complement(13288..14925)
                     /gene="pyrG"
                     /locus_tag="POB34_001551"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HDI5918527.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(15153..15944)
                     /gene="mazG"
                     /locus_tag="POB34_001552"
     CDS             complement(15153..15944)
                     /gene="mazG"
                     /locus_tag="POB34_001552"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDI5918528.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(16015..16350)
                     /gene="mazF"
                     /locus_tag="POB34_001553"
     CDS             complement(16015..16350)
                     /gene="mazF"
                     /locus_tag="POB34_001553"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HDI5918529.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(16350..16598)
                     /gene="mazE"
                     /locus_tag="POB34_001554"
     CDS             complement(16350..16598)
                     /gene="mazE"
                     /locus_tag="POB34_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HDI5918530.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(16676..18910)
                     /locus_tag="POB34_001555"
     CDS             complement(16676..18910)
                     /locus_tag="POB34_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDI5918531.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(18958..20259)
                     /gene="rlmD"
                     /locus_tag="POB34_001556"
     CDS             complement(18958..20259)
                     /gene="rlmD"
                     /locus_tag="POB34_001556"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDI5918532.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVSRHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            20316..23072
                     /gene="barA"
                     /locus_tag="POB34_001557"
     CDS             20316..23072
                     /gene="barA"
                     /locus_tag="POB34_001557"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HDI5918533.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRNGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(23304..24644)
                     /gene="gudD"
                     /locus_tag="POB34_001558"
     CDS             complement(23304..24644)
                     /gene="gudD"
                     /locus_tag="POB34_001558"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDI5918534.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAKRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(24665..26005)
                     /locus_tag="POB34_001559"
     CDS             complement(24665..26005)
                     /locus_tag="POB34_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDI5918535.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(26007..27359)
                     /gene="gudP"
                     /locus_tag="POB34_001560"
     CDS             complement(26007..27359)
                     /gene="gudP"
                     /locus_tag="POB34_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HDI5918536.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(27794..28243)
                     /locus_tag="POB34_001561"
     CDS             complement(27794..28243)
                     /locus_tag="POB34_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDI5918537.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEILLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(28261..29043)
                     /gene="truC"
                     /locus_tag="POB34_001562"
     CDS             complement(28261..29043)
                     /gene="truC"
                     /locus_tag="POB34_001562"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HDI5918538.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(29043..29372)
                     /locus_tag="POB34_001563"
     CDS             complement(29043..29372)
                     /locus_tag="POB34_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HDI5918539.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(29994..30539)
                     /gene="syd"
                     /locus_tag="POB34_001564"
     CDS             complement(29994..30539)
                     /gene="syd"
                     /locus_tag="POB34_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HDI5918540.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            30607..31455
                     /gene="queF"
                     /locus_tag="POB34_001565"
     CDS             30607..31455
                     /gene="queF"
                     /locus_tag="POB34_001565"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDI5918541.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVEFDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            31567..32931
                     /gene="ppnN"
                     /locus_tag="POB34_001566"
                     /gene_synonym="ygdH"
     CDS             31567..32931
                     /gene="ppnN"
                     /locus_tag="POB34_001566"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HDI5918542.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         8292 a         8665 c         7819 g         8308 t
ORIGIN      
        1 atcaggccta caagtcccca ggccagaacc gtaggcagga tcatccggca acacgttatt
       61 ggcaatctgc gtgaatgttt gtcaccagtg cctccagcac cactttccag tcatcaacca
      121 cgccaacatc tgcctgtgaa aataccgcag cgctggcgtc atgattaatc gccaccacaa
      181 acttgctatt gcgtacgccc gccattaacg ctgctgcgcc agaagcgcca acaacaatac
      241 acacctcagg tgccagcaga tgtccggaaa taccaatcac tttttcagca tcaacgccac
      301 cgttcatcac tcgcgcccga ctgtagccca cttcggctcc cagcttttct gccagcatag
      361 cgatttcctg gttatctgcc tctccgccct gccccaccac cagcacccgt ctggcctcag
      421 ctaatgggtc gcgagtaaca tttttcagat cctctgtgct aaccagccag tccggaagcg
      481 cacccgggac aatgatcagt tgttgcattc cagagggcaa cgtggcgttt ttcgctgctc
      541 ccgcctgacg cgccagggaa aggcatagcg ggcgtttttc tgtttgcagc gtcgccgtta
      601 gcgcatttcc ccagtgggat ttgcgcacgc ttacggttgg aatatctaac gaagtcacct
      661 ggcagatgct tgcaccatgt aaacgccagg ccagccgggt cgacagttca tcgccaaacg
      721 ttcccggcgg aaagagaaca acgtccgcag gcgttcgctg ccactgttcc accaacgcat
      781 ccagcacctg ttccgccact acaggctgag gttcaatctg ccagtgcgcc agggtacaac
      841 cgctgaaatc ctgcgccgcc agccagcttg ctattgcggc attctcctga tttattgtca
      901 caattgcgat attcatggct gcatcctttg gcgtaaatag tcccgccata gcttttgagc
      961 tttttctgct accgtttgcc catcaatcag cgtcgctccg cgtcgttgct ctgctcttgc
     1021 cagttgcaga cactgcatcg ctggcatttc ggcagcaaca gttttacgga taatttctgc
     1081 tttccccgca gccattcgct ggcgcattcc cggtactggc aaggccactt caccgcactg
     1141 acgcacggca atcaccgcag gcaagcgaac ccgggaacaa cgcaacccat gttcagtacg
     1201 ctgttcgagg gtgataaaca gcgcgtcgag agtgaaacgc tccacctggg tgaagcaggg
     1261 ccagcccagc atttccgcca gcaaaaatgg cgtttgccca ttctgccctt cgctgctttg
     1321 gcagccggta attatcagat ccagcggatt ctgatgctgc cattcggcaa tatggcgagc
     1381 gacaaattcc ggcgcaaagc gcagatctgc cgccgtctcc agcaatacag cttcctcaaa
     1441 cccaagagcc atgagatagc gcagccagtg caatgcccgt tcatccccca tgctcaatgc
     1501 ggttaaagac atcggcgtac cgttttttct ctgcgccaac agcagcgcgg cagcggcctg
     1561 ttcatcagca ccgagtaaac ttcgcagtag cgaaatatcc ggtccgcttt taccctgagc
     1621 cgccgcctgc cactcttttt ccgccagcat tccggcatcc ggttcggctt taaacgctaa
     1681 cagaatgttc attcgcacct cctacatttc ccgccgccac cagcgggagg gctttagttt
     1741 ctggtgccca tagccaggtc acaacaaaac caaccaacaa cactgtcgcc aggagcaata
     1801 aggtgacttg cattccccac tgcgccagca cccacggcag caggccagta cttaccgccg
     1861 cgccaagtcg actcatggcg gtggcaaaac cgacgcccag cgagcgaatg tcagtaggaa
     1921 aactttccgc aggcaaaatg cccaccagat tactgactgc cgaaatggtg gtgctgaaga
     1981 gaacaaaaag cagcagcgtt aatgaactgc cggaaggcaa acaggccatg actaccagcg
     2041 ttgccgccag cagcaaaaaa cttcccagca aaaatttgcg atgtgccagc aggtgcgtca
     2101 gaactaatcc cagcagcgcg cccacaatta acaacgcatt aagcatcagg ctggcagtca
     2161 gcgcatcttc cagaccaata gtctgggcga tagttggcag ccaggtataa atcacaaacc
     2221 atgggattac gaggcagaca aagaagacgc tgttaaacgc cgtgcgccgc cagtaacgcg
     2281 aagagaacaa ggttttgatg tgtttatggg tcgccgttac cacttcatcg cccagtaaaa
     2341 catggggacc aaaatagcga tgcacgatag cgtgagcttc tgcaaaacgc ccctggcgca
     2401 gtagccagcg tggtgattct ggcgttcccc agcgtaataa cgtaatcaac aacgcgggca
     2461 gagctgccga tgccagtagc cagcgccagg cctccgggtt ttcggaaata aagtgatgtc
     2521 cggcaatact tgccagcaca tagcctacgg tccacaccac gctgaatgcg cccagcaaaa
     2581 taccgcgatg gcggcgcggg gaaaattcag ccagcaaggt gtgacctact gaataatcgc
     2641 ctcccagacc aatgccaatc aaaatgcgca gtccaataag atgctctggc gtggtggcaa
     2701 aaaattgtaa aaacgaagca agcgtaatca gcaaaaagct gaaggtgaag attttttgcc
     2761 gaccaatatg gtcggagatc cacccaagaa ccaggctgcc aaggaacaaa ccaaggagcg
     2821 ccgagccgcc gatcattccc gccataaacg gcgtcagttg catggcgggc gtaagctgaa
     2881 taatggcgta accaatgacg ccgagaacat aaccgtcggt caggtgtgcg ccgaaagtga
     2941 gcgcagcaat gcggcagtga aaacggttaa gcggtaaatc atccattcgc accggtgaag
     3001 tgttcatctt taccccttca ttgtccggta ataagaacgg cttctcgcct gagaagccga
     3061 aaaggcaacg ccccgtcatc gcggaacgct gcctgacaca ttatttttca atcggataga
     3121 tagtacccgt attcataatg ccattaggat cgaactgctt tttcagccct tccagcaacg
     3181 cccacgcgct gccgtgttcc agtttgctcc agtgaacgcg atgtttaccg ataccgtggt
     3241 ggtgcaccat cgaaccgccg aggcgaatgg tttcttcaca gatgatcttg ttgagcggat
     3301 tgtggtactt gtcgatttcc tcttccggct tacagtcaac gacgttgtaa tcgtagacga
     3361 agtacatgtt ggtgccgttc tgatagctat gagaggaatg accgcccagc atggtgatgt
     3421 cgtcggcgtg cgggaactca gtacgaatac ggttaataac gctttcgtag atttcgtgga
     3481 tgcagctcca gcagccggac acttcagtgg taaagcccat gttgccggtt ttgaggatct
     3541 gcacacgttc ggcagccact ttatccggtc cccagttcag gttgttaaac caggtttcga
     3601 tcagtttgct gtccacgcgc tggcactgcg ggtagcgggc aacgatttcc gcaatccctt
     3661 cgcccgtcgc cttcgcaatg cgaggattgc cttcagccat aaagatcagc acacatttac
     3721 cgtcggcaaa atgggtgaag tgttgggtgc catcttcagc gtcatacaaa cgagcgatcg
     3781 acggacgata cccttccacc atgacttcac gcaggatgtt gaagccggtt ttcatgtctt
     3841 ccaggatata gccgtagaag aggttgtttt ccggggtgaa tttaaagatt ttcactgtta
     3901 cttcagtgat atagcaaagt gcaccttcgt taccgatgat gatgtggcga atgtccgggc
     3961 cagccgcgcg gcgtggcacg tttttaatgc gtgtgacggt gccatcagcc aatactgctt
     4021 ccagaccaac gaccatatct tcgattgcgc cgtagagtgt ggagaactgc ccgatactgc
     4081 gggttgctac caggccgccc atctgcgcca gcggctttga ctgcggagaa tgccccgtgg
     4141 tgtaaccttt ttcacgcaac gcattttcca gcacctccag cggaacgcca cattgcgccg
     4201 tcgcctgcat attctcaata tcaatattaa tgatttgatt catggcggag ccgtcgagca
     4261 ccaccgagtt ttccacaacg gtttccagcc caccttcagt ggcggaagcg ccggtacgcg
     4321 gcacaccgtt aattttgtgc gcattcataa aattcagcac acgggagact tgttctgtgg
     4381 aaccgagttt tacgaccgct gccggaatcg gcaaagtata aataccatga atatccggaa
     4441 atttacgaaa acggtcaata ctgttcttct ttaatactgt ttcatcggta attacgcgat
     4501 ctgcaccaac aatttccttt agttggtcga caatcgctgc gcgagataaa gacatagtaa
     4561 ttccttcctg ataataaaaa tggaataatc attaataaga gaaaacagaa accaccgtta
     4621 tgggttagcg cactaaataa ccgccatcaa ccactaataa atgcccgttg acataattcg
     4681 atgccggact tgcgaggaat acggctgcgc ccattaaatc ctgagtatcg ccccaacggt
     4741 ttgccggaat atgatcaaga acgcgctgat tggtttctgg attactgcgt gtcgccagcg
     4801 taatatctgt tgcataatag ccaggggcga taccatttac ctgaatatta tattgaccta
     4861 gttcatcaca ataagctttg gtgaacccgg caagagcatg tttagtggca gaatatgcag
     4921 gtgaccattg tccacctaag taagagaaca atgaacagat attaataatt ttgccgcttt
     4981 tctgcgggat cataattttt gcagcttcat agcttaactc gaatgcggcg gtcaggttca
     5041 catcaatcat cggatcccag tcggcacgac cgaagtccag caccttattc agcttacaaa
     5101 taccggcatt gttaaccaga atatcaactg taccgaaacg ctcacagcta gcagcgataa
     5161 tcttctgcgg cgcgccttct gcggtgatat ccacctgcat gaagtccacc tcaacaccct
     5221 gtttttcaat catttccttt gtttcgccgt tatctttgac gaaactagga ataaagatat
     5281 ttgcgccagc tttggccaac gccatggcaa atgcctggcc taaaccgcta ttcccaccgg
     5341 taacaattgc ggttttacct ttcagggaga aaaaatccat tgagaacgca ttgagagatt
     5401 cgattgacat agttagctcc aatttccgta aggcaaaaaa aaagaaaggt aacttccccc
     5461 gcgagaggga agttaccttt ctcattatct ctggtaactg tagataattt ttagcataac
     5521 ggcataactg ctaatgtgat cgtaatcaca gtgtgatatt cgtattgtca aatagtttta
     5581 aaaagcagaa gcgtgattac tctcacacaa cacctctgct ttccatgtta tttcttattg
     5641 caattactag agacctgaga aaagtaatcc acctgaaagg gcggcttact tttctctttt
     5701 ttttgtctat tacaaatggg aatacgatat gcaacacaac tcatatcgcc gttggataac
     5761 cctcgcgata attagtttta gcggcggcgt tagtttcgac ctggcttatt tacgttatat
     5821 ttatcaaatt cccatggcga aatttatggg attcagcaat accgagatag gtttaataat
     5881 gagtaccttt ggtattgcgg ctattattct ttatgccccc agcggcgtta ttgccgataa
     5941 attttcacac cgcaaaatga ttacttccgc gatgatcatt accggattac tgggtctgtt
     6001 aatggcaacg tatccaccgc tctgggtaat gctctgtatt caggtcgcct ttgcgataac
     6061 aacgatttta atgctgtggt cggtgtcgat taaagccgcc tcgttgctgg gcgatcatag
     6121 cgagcaaggg aaaattatgg gctggatgga agggctgcgc ggcgtcggtg taatgtcgct
     6181 ggcggtgttt accatgtggg tcttttctcg ctttgcaccg gatgacagca ccagcctgaa
     6241 aacggtcatt atcatctata gtgtggttta catcttgttg gggattctgt gctggttttt
     6301 tgttagcgat aacaacaacc tgcgcagtgc caataacgaa gaaaaacagt cattccagct
     6361 tagcgacatc ctggccgttt tgcgcatcag caccacctgg tattgcagca tggtgatttt
     6421 tggtgtcttc accatctacg ccattctgag ttactccacc aactatctga ccgaaatgta
     6481 cggcatgtct ctggtggcgg cgagctacat ggggattgtg atcaacaaaa tcttccgcgc
     6541 gctgtgcggc ccacttggcg gcattatcac cacctacagt aaagtgaaat cccctacccg
     6601 cgtgattcaa atcctttcca tcatcggttt gctggcgttg acagccctgc tcgtcacgaa
     6661 ctctaacccg caatcggtcg cgatggggat tggcctgatt ttactgctgg gattcacctg
     6721 ttacgcctca cgcgggctgt actgggcctg ccccggcgaa gcgagaacac cgtcttacat
     6781 tatgggcacc acggtaggta tttgttcggt gattggattc ctgccggatg tcttcgttta
     6841 cccgattatc ggccactggc aagacaccct gcccgctgca gaagcctacc gcaatatgtg
     6901 gctgatgggc atggcggcgc tcggcatggt gattgtcttt acctttttgc tgttccaaaa
     6961 aattcgtact gctgatagcg cccccgcaat ggctagcagc aagtaagcca ggaaaattac
     7021 aggagaaaaa tcatgtcgaa aaaatacatc atagggattg atggcggaag tcagagcaca
     7081 aaagtggtga tgtacgatct ggaaggtaat gtggtttgcg aaggtaaagg cttattacag
     7141 ccgatgcaca cgccagatgc cgatactgca gaacatcctg acgacgattt atgggcatca
     7201 ttatgttttg ccggtcacga tttgatgagt cagtttgccg ggaataaaga agatattgtc
     7261 ggtattggtc tgggatccat ccgttgctgc cgtgcgttat tgaaagccga tggcacgcca
     7321 gctgcgccgt tgattagctg gcaggatgca cgcgttacac gcccttacga acatacgaat
     7381 cctgacgtgg cgtatgtcac ctctttttcg ggttatctga cgcatcgctt aaccggcgag
     7441 tttaaagaca atatcgccaa ctattttggt cagtggccgg tggattataa gagctgggca
     7501 tggagcgaag atgctgcggt aatggataag tttaatatcc cccgtcatat gctgtttgat
     7561 gtgcaaatgc ctggcaccgt cctcggacat atcacaccac aagccgcact ggcgacacat
     7621 ttcccggcag gactgccggt tgtttgtacc accagtgata aaccggtaga agctctgggg
     7681 gccggattac tggatgatga aactgcggta atttctcttg gcacttacat cgcattgatg
     7741 atgaacggca aagcactgcc gaaagatccg gtggcgtact ggccgattat gtcttctatt
     7801 ccgcaaacat tgctgtatga aggttacggt attcgcaaag gtatgtggac ggtgagctgg
     7861 ctacgcgaca tgttaggcga gtcgttaatt caggatgcca aagcacagga tctttcaccg
     7921 gaagatttgc tcaacaaaaa agcttcttgt gtgccgcctg gctgtaatgg tctgatgacg
     7981 gtgctggact ggctgaccaa cccgtgggaa ccctacaaac gtgggattat gatcggcttt
     8041 gattccagca tggattacgc atggatatat cgttcgatac tggaaagcgt ggcgctgacg
     8101 ctgaagaaca attacgacaa tatgtgtaat gaaatgaatt actttgcaaa gcatgtgatc
     8161 attactggcg gcggttcgaa cagcgatctg tttatgcaga tttttgccga cgtgttcaac
     8221 cttccggcac gacgtaacgc cattaacggt tgtgcaagcc tgggggcagc gattaataca
     8281 gcggtaggtc tggggctata cccggattac gcaacggctg tcgataaaat ggttcgcgtg
     8341 aaagatatct ttatgcctgt tgagagcaat gccaaacgct acgacgcgat gaataaaggc
     8401 attttcaaag atttaaccaa acacactgat gtgattctga aaaaatcgta tgaagtgatg
     8461 catggggaat tggggaatgc ggattcgatc cagagctggt cgaatgcgtg aatgaggtag
     8521 gccaggcgga aaactgcccg gcctgaacat acctaaatag ttatccccgc tgacgcgggg
     8581 aacataagtt cgacaactta aaaaatgata acaagtccgg tttatccccg ctgatgctgg
     8641 gaacataata aaaactttaa ctagttcggt ttatccctgt ttttacaagg aatattaact
     8701 acactcgatg tctttagaat aaaagacaat acgaatcgca ctctatcaat tttcacttaa
     8761 agtatgaact ggatatcttt attcataaga actattcatc aacagcgtat agaggcggtt
     8821 atgtaaaacc actcattagc ctcaaaacaa agacaaattt ccagatttat tacttaatca
     8881 atgaataaca agcttaaaaa atacactgtt attctaaaga aaaaattaac aggcattaat
     8941 taatattaaa aaaaataata tattaaaatt tcttaacggt ttaaggatta tacaaataaa
     9001 actttgatta aatttgattt tttgtatcga taattgtgaa cttatctggc atggggaact
     9061 attttgaaca tgaggtgtta cgtggatatg ttgcttatta caagtacagc taatataaaa
     9121 acttgagaaa gagataacgg gttatatggt ggtttatccc cgctggcgcg gggaactcat
     9181 atacagcaaa ccttcagaat gcattccggg cggtttatcc ccgctggcgc ggggaactcg
     9241 ccgtctgcct ccagcccagc cctggatatt tcggtttatc cccgctggcg cggggaactc
     9301 tggttcactc acgcaacgtc tgagcgcgtt gacggtttat ccccgctggc gcggggaact
     9361 cagggccgcc gctaccccag aagtgccact ccccggttta tccccgctgg cgcggggaac
     9421 tcgcgtatcg tctcgttatt gcgccgcccc aactcggttt atccccgctg gcgcggggaa
     9481 ctcagtatgc gttcaatcac tgtcaggcct acttccggtt tatccccgct ggcgcgggga
     9541 actcagttgc ccgatggaca ctcccagacc atcagccggt ttatccccgc tggcgcgggg
     9601 aactcactaa acttaatgat ggccgttaca gcgtggacgg tttatccccg ctggcgcggg
     9661 gaactctgta aacataacct attattaatt aatgattttt taagccagtc acaatctacc
     9721 aactttacag tatcacacaa acaacacatc cattatgtta aagagtactt tattcattga
     9781 ttaaaaaggt aaatatttaa aataactcta tacaaattaa aatctaccaa actttaccgc
     9841 aataattttc actccagcga aaaattaatg ccacagaatt tgtagagaat gctaatgatt
     9901 agccctggat gggtaaaatg gaggagtttt cagaggcgac aaacaatata aatgagtaga
     9961 agtctttaca ggttccaccc atttataacg cttataaatg tttaatcagg caatatttag
    10021 atatttatgt gtttgcatcg acaaacgcca attacgcgca atgcaggttt caatgcacaa
    10081 acgtgtggca tcatcctttt ggctaatcgg ctgcagtgca atgactcgcg gtttatcatc
    10141 ggtcagcgtt gccagtagtt catccagtgc ttcaatatcg cgtacgcgcc ccactggatg
    10201 cttgatttcg ttggctcgct ccagtgcctg tgacaacact tcatagccgc cgcgcatgtt
    10261 cagctttggc gatacggtaa cccaggtatt cggtgtgcag cgtacctcat gagtaccact
    10321 ggtttcgatc tggcagctaa aaccgttctt ttcgagcaga tcagtcagtg gcagcaaatc
    10381 atgaatgcaa ggctcaccac ccgtaatcac cacatgccgc gcggtgtatc cctggcgact
    10441 aatgacagcc aacaagtctt cactgctcgc cgccccccac ttatcactct ctttggtctt
    10501 cgccagaatg ctgaaaaggg agacttcccg atcctcaagc ttttcccagg tgtgtttggt
    10561 gtcgcaccag gcacagccaa ccgggcatcc ctgtaaacga ataaaaatgg cggggacgcc
    10621 ggtaaagtaa ccctcacctt gcagggtctg gaacatctcg ttaatcgggt actgcatagc
    10681 attctctgtg aagtggataa ttgttaatta ttgcagatcc tgccacaaca atcatgtctt
    10741 attaacattc tgttacaggc agatttaaag aaaaatgcga aacatatcgt taataattaa
    10801 agggagtaac gtattatgtc agaagaaaat aaagaaaatg gatttaatca tgtcaaaaca
    10861 ttcaccaaaa ttatatttat tttttctgta ttagttttta ataataacga atctaaaatt
    10921 accgatgccg ccgtcaattt atttatccag atttaatgag aaatttataa tgcgctattt
    10981 catactaatg ttcactttcg tgtgttcctt tgttgcagcc caaccaacta ttgtcccaca
    11041 attacagcaa caagttacgg atcttacgag tagcttaaac tcacaagaaa agaaagaact
    11101 gacgcacaag ttagaatcta ttttcaataa tacccaagtg caaattgctg tattaattgt
    11161 tcccacaacc aaagacgaga caattgaaca atatgctaca agagtttttg acaattggcg
    11221 tttaggagat gccaaacgta atgatgggat actgatcatt gttgcctggt cagatcgtac
    11281 tgtccgcatc aaggtaggtt atgggctgga agaaaaggta accgatgctc tggctggaga
    11341 tatcatccgt agcaacatga tacccgcatt taaacaacaa aaattagctc agggattaga
    11401 gctagctata aacgctttga ataaccaact cacttcacaa caccaatatc cgactaatcc
    11461 ttcagaaagt gaatcagcgt cttccagtga tcattattac tttgctattt tttgggtatt
    11521 tgcagtgatg ttcttccctt tctggttttt tcatcaaggc agtaattttt gtcgcgcatg
    11581 taaaagtgga gtctgtattt cagcaatcta tcttttagat ttattcctgt tctccgataa
    11641 aattttttcc attgctgtat tttccttctt ttttactttc accatattta tggtctttac
    11701 ctgtttatgt gtacttcaga aaagagcatc tggtagaagt tatcattcag acaacagcgg
    11761 ttccgctgga ggctcagata gcggcggatt tagcggcggg ggcggttctt ctggaggtgg
    11821 cggcgcatcc ggccgctggt aactcaacca taaaaaatgc cagcccggag gctggcattt
    11881 ttaaatcaga taaagtcagt cttatgcctg gcctttgatc tctttacgac cgttgtacgg
    11941 tgctttttcg cccagagctt cttcgatacg aatcagctgg ttgtatttag caacacggtc
    12001 agaacggctc atagaaccag ttttgatctg gcctgcagca gtaccaacag ccaggtcagc
    12061 gatggtagcg tcttcagttt cgccagaacg gtgagagata actgcagtgt agccagcatc
    12121 tttcgccatc ttgattgcag ccagagtttc ggtcagagaa ccgatctggt tgaatttgat
    12181 caggatggag ttagcgatac ctttttcgat accttctttc aggatcttgg tgttggttac
    12241 gaacaggtcg tcaccaacca gctggatttt gtcgcccaga actttggtct ggtatgcgaa
    12301 accgtcccag tcagattcgt ccagaccgtc ttcgatagaa acgatcgggt actgtttggt
    12361 cagttcttcc aggaagtgag tgaattcttc agaggtgaac gctttgttgc cttcgccagc
    12421 cagaacgtat ttaccatctt tgtagaattc agaagctgcg cagtccatcg ccagagtgat
    12481 gtctttgccc agttcataac cagcagcttt aacagcttca gcgataacag ccagagcttc
    12541 agcgttggaa cccaggttcg gcgcatagcc accttcgtca ccaacagcag tgttcatgcc
    12601 tttcgctttc agaacttttg ccaggtgatg gaaaacttca gaacccatgc ggatggcttc
    12661 tttcacagtt ttcgcgccaa ccggctgaat catgaattcc tggatatcaa cgttgttgtc
    12721 agcgtgctca ccaccgttga tgatgttcat catcggaacc ggcatagagt atttgcccgg
    12781 agtaccgttc agttcagcga tgtgctcgta cagcggcata cctttagcag ctgcagcagc
    12841 tttggcgtta gccagagata cagccaggat tgcgttcgcg ccgaatttgg atttgttttc
    12901 ggtgccgtcc aggtcgatca tgatcttgtc aatgccagcc tgatctttag catctttgcc
    12961 aatcagcgcc tgagcgatcg ggccgtttac cgcagcaaca gctttggtta cgcctttacc
    13021 caggaaacgg gatttgtcgc catcgcgcag ttccagagct tcacgggaac cagtagaagc
    13081 acctgacgga gcagctgcca taccgacgaa accaccctcc agatgtactt cggcttcaac
    13141 agtcgggtta ccacgggagt cgatgatttc acgaccgatg atttttacga ttttggacat
    13201 taggttttcc tcaagtcact agttaaactg aaactccaga caaacaacgc gtacccaggg
    13261 tacgcgttgc cgctctaact tttttactta cttcgcctga cgtttctgga actcgctggc
    13321 ggctttcaca aagcctgcaa acagcgggtg accatcacgt ggagtagaag taaactccgg
    13381 atggaactgg caagccacga accacgggtg attcggaact tcgatgatct cgaccaactg
    13441 atcatccccg gaacggcccg caacgcgcag acctgcatct tcaatctgtt tcaacagcat
    13501 gttgttgact tcgtaacggt gacgatgacg ctcaacaatt gtcggcgcat tgtacagctg
    13561 gcgaaccagg ctatcgtcaa ccaactggca ctgctgtgcg ccgagacgca tggtaccgcc
    13621 gagatcgctc ttctcgctac gaacttcaac gttgccgttt tcatcgcgcc actcggtaat
    13681 cagcgccaca accgggtact tacagtctgg cacaaattcc gtagagttgg cgttctccat
    13741 gttggcaaca tggcgagcgt aatcaattaa cgccacctgc atacccaggc aaatgcccag
    13801 ataaggaata ttgttctcac gcgcaaaacg cgcggtcgta atcatgcctt ctacgccacg
    13861 atagccgaaa ccgccaggta cgaggattgc gtccagacct ttaaggattt caacgccgcg
    13921 cgtttcaaca tcttgtgaat cgatcagttt gatgttgacg ctgacacgat tcttcagccc
    13981 accgtgtttc agtgcttcga tcactgattt ataagcatcc ggcagttcaa tgtacttgcc
    14041 gaccataccg atggtgactt cacttaccgg gttcgcttct tcgaagataa cctgttccca
    14101 ttcggacaga ttcgcttccg ggcagtttaa gctgaatcgt ttacaaatat aatcgtccag
    14161 cccctgagat ttcaacaggc ccggaatttt atagatggaa tcgacgtctt tcagagaaat
    14221 aaccgctttt tccggaacat tacagaacaa tgcaatcttc gcacgttcgt tcgccggaac
    14281 agcgcgatct gaacgacaaa tcaggatgtc aggctggata ccgatggaga gcagctcttt
    14341 tacagagtgc tgagtcggtt tggttttgac ttcaccagac gctgccatgt acggcaccag
    14401 cgtcaggtgc ataaacagag tgtgctcacg gccaatttca acagccatct ggcgaatcgc
    14461 ttcgaggaac ggcaaggatt cgatatcacc tactgtaccg ccgatttcta ccagtactac
    14521 gtcatgacct tcgccacctt ccagcacgcg ctctttgatt gcgttagtga tgtgcggaat
    14581 aacctgcacg gttgcgccga ggtagtcacc gcggcgttct ttacgcagaa cgtcagagta
    14641 gatacgaccc gtggtgaagt tgttgcggcg gctcattttg gtacgaatga aacgctcgta
    14701 gtgccccagg tccaggtcgg tttcagcgcc gtcttcagta acgaacactt ccccgtgttg
    14761 gattgggctc atagtacctg gatcgacgtt gatgtaagga tccagtttca tgatggtcac
    14821 attgaggcca cgggcttcaa gaatggctgc gagggaggct gcggcaatgc ctttacccag
    14881 agaggatacg accccgccgg tcacaaaaat atagttcgtt gtcatgctga acctgagaag
    14941 ttaggttgaa aagacgatgg aataaccagg acgggaaagc agtatacccg aacatgacct
    15001 gtgccacaaa ctttcattat ccctcctctt cgccagcgca ctattgaaat caggagtgag
    15061 aaaatagccc ctttggggta aatgtttttg acgcaaatca agcgcttgtc atttaaaaaa
    15121 tgacacaaat ggcgcttgac cgcgtaattc ccttagagat caatttcctg ccgttttacc
    15181 tgttgccaga cttcttccat tgtttcgagg tcaacacctg tcatttccag tccacgcgcg
    15241 gcaacaatac gctccacttc gcgaaaacga cgctcaaatt tttcgttcgc tttttgcaat
    15301 gcgatttccg cttttgtccc taaatgacga gccagattga ccgtggcaaa cagcaggtcc
    15361 cccatttcct cctccagttt agcctggtcg acaacagcct gccgcgcttc gtacatcacc
    15421 tcgtcgatct cttcgtagac tttatcgact accggaccaa gcgtcgtcca atcgaagcca
    15481 acgttggcgc aacgtttctg gattttttgc gcacgcatta aagccggtaa actacgagga
    15541 atatcgtcca gcgccgaatg ctgcgctttc tgcgcgcgct cttcggtttt gatttgctcc
    15601 caacgggcaa gcacttcact actgttttcg gcagaactat cagcaaaaac atgcggatgg
    15661 cgacgctcta atttatcgct aatagcagcg caaatatcat taaagtcaaa gcgcccttct
    15721 tcctgagcca tttgcgcgta aaacaccacc tggaatagca gatcgcccag ttcaccgcga
    15781 agatcgtcaa aatcttcacg ggcgatggcg tccagcactt cgtaggtttc ttcaagggtg
    15841 taaggcgcaa tggtggcaaa tgtctgctct ttatcccacg ggcagccgtt ttccggatcg
    15901 cgcaggcgct gcataatagt gagcaaacgg tcgatttgat tcattgaatt gtcctgaaaa
    15961 ttgcgggtct gtcaggtgga aacctgtgac cagaatagaa gtgagttagt aacactaccc
    16021 aatcagtacg ttaattttgg ctttaatgag ttgtaattcc tctggggcaa ctgttccttt
    16081 cttcgttgct cctcttgccc gccaggcgat actttttacc tgatcagcta acgctacgcc
    16141 atcacgttcc tgaccggata aaacaacttc gaacggatat ccttttgatt gcgttgtaca
    16201 aggaacacac agacacatac ctgttttgtt gttgtacatg aaaggactca ggacaacagc
    16261 tggacgatgt ccagcttgct cgctaccttt tgtcgggtca aaatcaaccc aaatcagatc
    16321 gcccatatcg ggtacgtatc ggcttaccat taccagactt ccttatcttt cggctctccc
    16381 cagtcgatat tctcgtggag gttttccggc gtgatgtcgt tgaccagttc agcaagcgta
    16441 aatacgggct ctttacgcac tggctcaata attaatttgc catccaccag gtcaatcttc
    16501 acttcatcat caatattcag attgagcgcc tgcattaacg tagccgggat ccgcaccgcc
    16561 ggtgaatttc cccaacgctt tacgctactg tggatcataa ccctttcctc aaaccgctat
    16621 catatgtaga tacagtatat atcaatctac attgtagata cgagcaaatt tctgcctaac
    16681 tcccgtgcaa ccgacgcgcg tcgataacat ccggcacctg gttgagttta cccagcacgc
    16741 ggcccagcac ttgcaggttg taaatctcaa tggtcatgtc gatggtcgcc agttgctgtt
    16801 tggtgtcgct acggctggca acgccaagca cgttcacctt ctcgttggcg agaatggtcg
    16861 tgatatcacg taacaaccca ctacgatcat tagccaccac gcggaccacc agcgaatatc
    16921 cggcggagta gctctcaccc cataccgcgt caacaatgcg ttctggcgca tgggagcgca
    16981 gttccgccag ttgttcgcaa tcggcgcggt gtactgaaat accgcgtccc tgggtaatga
    17041 agccgacaat ctcatctcca ggaatcggct ggcagcagcg cgcgatgtgg tgcatcaggt
    17101 taccaacacc ttcaactacc acacgaccgt tatctttact gcggttttgc ggtgtgtagc
    17161 ttttttgctg aagctgtttc agcgcggcgg cgtcctgctc ttcggcactc ggcttattaa
    17221 attgcgattg caggaagttc accatctgat tgagacggat atccccgcca ccaatcgccg
    17281 ccagcaactc gtcgacatca ttgaagttgt aacgcggcaa cagatgtttt tctgcttctt
    17341 tcaggctgat ccccagatgt tccagctcgt cgtcaaggat ttgccgccca gccagaatgt
    17401 ttttgtcacg gtcctgttta cggaaccagg cgtgaatttt cgaacgccca cggctggttg
    17461 tgacgtaacc gaggtttggg tttaaccagt cacggctggg gttcggctgt ttctgggtga
    17521 taatttcaat ctggtcgccc atctgcagct ggtaggtgaa cggcacaatg cgcccgccaa
    17581 tttttgcccc gatgcagcgg tgtccgacat cactgtggat gtggtaagcg aagtccagcg
    17641 gcgttgatcc cgccggcaaa tcaacgacat cacctttcgg cgtaaagacg tacacccggt
    17701 cgtcaaagac ctgactacgt acttcgtcga gcatttcgcc ggaatcagcc atctcttcct
    17761 gccacgcaat cagtttacgc agccaggcaa tccggtcttc atgtcccgaa cgtgcgccgc
    17821 cagcagccgc gccctcttta tatttccagt gcgcagcaac acccaactct gcatcttcat
    17881 gcatctgttt ggtgcggatt tggatctcaa cggtttttcc acccggcccc agaaccacgg
    17941 tatgaataga ctgataaccg tttggtttcg ggttagcgac gtaatcgtca aactcatccg
    18001 gcaggtggcg atagtgagtg tgcactatcc ccagtgcggc ataacaatcc tgtaaacgct
    18061 cggcgacaat acgtaccgca cgaacatcaa acagctcatc gaaggcgagg ttctttttct
    18121 gcattttacg ccagatgctg tagatgtgtt tcggacgacc atacacttcg gctttaacgc
    18181 cttcagcttt catctcagcg cgcagatgac cgacaaactc ttcgatatag tgttcgcggt
    18241 cgagacggcg ttcatgcaac agttttgcga tgcgtttgta ttcggtcggg tgcagataac
    18301 ggaagcagta atcttccagc tcccatttca gttgcccaat cccaagacgg tttgccaacg
    18361 gcgcgtagat attggtgcac tcttttgccg ccagtacgcg ttcatcttcc ggcgcatctt
    18421 ttacttcccg cagatgagca atacgctccg ccagtttgat gaccacgcag cgaaaatcat
    18481 cgaccatcgc caataacatc cggcgaacgt tatcgacctg ttcggaggaa acagaatcag
    18541 tgtgcgtcgc tttcagctgg cggatcgccg ccatatcacg cacgccgtga ataaggttaa
    18601 cgaccgactt accgacgctc tcacgcagca catcttcgct gactacgttg gcatccgcca
    18661 gagggaaaag cagcgccgcc cgcagcgtgt caatgtccat acttaatgtc gagaggatct
    18721 ccaccatctc aacaccacgc cacaataaca gactggcatc cggatgcccc tgcgtctgtt
    18781 gcagacaata cgcccaggtt tcggctaagc actcacacga cttctggctg gtaataccca
    18841 gacttgcgat ccatttttcc ggatcaaatt caccagcctt attgatatgt gcacttctta
    18901 ccgcaaccat cgtcctctcc tttagggacc agacctgccg aaatcggcaa atcgcaacta
    18961 tttaacgcgc gagaaaagta ccatcgattc cagatgtccc gtgtgtggga acatatccag
    19021 catcgccagt cgcgcaatgg tatatcctgc ttttaataac gcttcgctat cccgagccag
    19081 cgttgcaggg ttacaggata cataaactat acgaataggt tccagtttta taatttgctg
    19141 cataacacct gcggcacctg ctcgcgccgg gtccagcaac actttatcga agccgttttt
    19201 cgcccacggc tgctttgtga catcttcttc aagattttcg tgataaaacg tcacattctg
    19261 taagccatta agacgcgcat tctgctggcc tttttccacc agcgccggaa caccttctac
    19321 accgaccaca ctggcagctt gtgtcgccaa tggcagtgta aagttgccca taccgcagaa
    19381 cagatccagt acgcgatctt caggttgcac atccagccat tccaacgcac gcgctaccat
    19441 tttttggttc acacccgcat tgacctgaat aaaatcgcgc gggctaaaag ttaagcgcaa
    19501 cccgtttgag tcataccagg gcatctcacc agagacggtt tcgagtatct cactatcggg
    19561 ggcgagatac agatccaggc cttcagaatg cgaaaagcgt tccagttttt cgcgatctgc
    19621 cgaacttagc ggtgcggtat ggcgcaaaat catcagcgtg ccgctggttg cctgtaccag
    19681 ttcaacatga ccaagatggc gcatagcttg taagctgccc agacatgccc tgactttggg
    19741 cagcaatgct tcaagttggg gcgctaaaat ggggcattgt ttgacgtcga caatgtcact
    19801 ggagcccgct ttgcgaaacc ccatctgaag ttgctgtgtt ttcggtaagt agttcagact
    19861 taaacgcgcg cgacggcgat agccccaggg aacatcggcg atcacttcag agacatcgtg
    19921 tttcattaat cgggcgagtg ccgcactttt gcttcgctgc tgtaaatcca cgctggcgtg
    19981 ttgttgctga cagccaccgc atacgccaaa atgaggacag cgtggcgttt cgcgttccgg
    20041 gctatcgctt aaccggcgta cgactttagc gcgggcatac tgttttttat cttcagtaac
    20101 agtaacttcc gcgttttcct gcggcaataa tccggggata aatagcgttt tgccgttatg
    20161 tcgcgacacg ccctgaccaa aagagtcgag gtcgttgact gaaacggtta tgatctgacg
    20221 cgtcgtcgtg cgtcgttttg cagagtagaa ttgcgccatt ggcgagactt tctcaattta
    20281 acagtgtgac cttaattgtc ccataacgga actccatgac caactacagc ctgcgcgcac
    20341 gcatgatgat tctgatcctg gcaccgaccg tccttattgg tttattgctg agtatctttt
    20401 tcgtcgtgca tcgctataac gacttgcagc gtcaactgga agatgccggt gccagcatta
    20461 ttgagccgct tgcagtttct actgaatatg gcatgagcct gcaaaatcgc gaatctatcg
    20521 gtcagttaat aagcgtactg catcgtcgcc attccgatat tgttcgcgcg atttcggttt
    20581 atgatgaaaa taaccgactc tttgtcacct ccaattttca tcttgatccc tcatcaatgc
    20641 agctcggcag caacgtgccg tttcctcgcc agctcactgt cactcgtgac ggcgatatta
    20701 tgatcctccg cacgccgatt atttctgaga gttactcccc cgacgaatcg cccagtagcg
    20761 atgccaaaaa tagtcaaaat atgttgggat atattgcgct ggagctggat cttaaatcgg
    20821 ttcgcttgca gcaatataaa gagatcttta tttccagcgt gatgatgctg ttttgtatcg
    20881 gtattgcgct tatttttggc tggcgcttaa tgcgcgatgt aaccggtccg attcgcaaca
    20941 tggtgaatac cgtcgaccgc atccgtcgcg ggcaactcga cagccgggtg gaaggattta
    21001 tgctcggcga gctggatatg ctgaaaaacg gtatcaactc gatggcaatg tcgctggctg
    21061 cttatcacga agagatgcag cacaatatcg accaggcgac gtccgatctg cgtgaaacgc
    21121 tggagcagat ggaaattcag aacgttgagt tggatctggc gaaaaagcgc gcccaggaag
    21181 cggcgcgtat taaatccgag tttctggcaa atatgtcaca cgagctgcgt acaccactga
    21241 atggtgttat tggctttacc cgcctgacgc tgaaaacaga attaacacca acgcagcgcg
    21301 atcacctgaa tacgattgaa cgttcggcaa ataatttgct ggcaattatt aatgatgttc
    21361 tcgacttctc gaaactggaa gcaggtaagc tgattctgga aagtattcca ttcccactac
    21421 gcagcacgct ggatgaagtc gttactctgc tggcacattc ttctcacgat aaagggttgg
    21481 aactgacgct caatattaaa agcgacgtgc ctgataacgt gatcggcgac ccgctgcgat
    21541 tacagcaaat catcactaac ctggtgggga atgcaattaa attcaccgag aatggcaaca
    21601 ttgatattct ggtagaaaaa cgtgcgctga gtaataccaa agtgcagatt gaagtgcaga
    21661 ttcgggatac cggcattggt attcctgaac gcgatcaatc gcgcttattc caggccttcc
    21721 gacaggctga tgccagtatt tcccgccgtc atggtggcac cgggctgggg ctggtgatta
    21781 cacaaaaact ggttaatgaa atgggcggcg atatttcgtt ccatagccag ccgaatcgcg
    21841 gttcaacttt ctggttccac attaatctcg atctgaaccc gaacattatt atcgaagggc
    21901 catccaccca gtgcctcgca ggtaagcgcc tggcctatgt cgaaccaaac tccgcagcag
    21961 cgcaatgcac gctggatatt ttaagtgaaa cgccgctgga agtggtttat agcccaacgt
    22021 tctccgcgct gcctcccgcg cattacgaca tgatgttgtt aggcatcgcg gtgaccttcc
    22081 gcgagccgct aacaatgcaa catgagcgat tagcgaaagc ggtatcgatg accgatttcc
    22141 tgatgctggc acttccttgc catgcacaag tcaatgctga aaaactcaag caagatggta
    22201 tcggcgcgtg tctgctgaaa ccattaacac ctacacgcct gttgcctgcc ctgacggaat
    22261 tttgtcatca caaacaaaac acgcttttgc ctgtaaccga tgaaagtaag ctggcaatga
    22321 cagtcatggc ggttgatgac aaccccgcta acctgaaact tatcggcgca ttgctggaag
    22381 atatggtgca acatgtggaa ctttgcgata gcgggcatca ggcggttgaa cgggcgaaac
    22441 agatgccgtt cgatttgatc ttaatggata ttcaaatgcc ggacatggat ggcattcggg
    22501 cctgcgaact catccaccaa ctcccgcatc agcaacaaac gccggttatc gcggtaacgg
    22561 cgcatgcaat ggccgggcaa aaagagaagc tgcttggcgc agggatgagc gattatctgg
    22621 cgaaaccgat tgaagaagag cgattgcata atttgttgtt gcgctacaag cctggcagcg
    22681 gtatttcctc tcgcgtcgtg acgcccgaag tcaacgaaat tgtggtgaac ccgaatgcga
    22741 ccctcgactg gcaactggca ctacgccagg cagcaggaaa aaccgattta gcgcgcgata
    22801 tgctgcaaat gttactcgat ttcctgcctg aagttcgcaa caaagttgag gaacagctag
    22861 ttggagaaaa cccggaaggc ctggttgatt tgattcataa actgcatggc agttgcggct
    22921 atagcggtgt gccacgtatg aagaatctct gccaactgat cgaacaacag ctacgtaacg
    22981 gtactaaaga agaagatttg gaaccggagc tgctggaact gttggacgag atggataatg
    23041 tcgcgcgcga agccagcaaa attctcgggt aatggatgtc ttgtcgagta gcaatcggct
    23101 ttttatgacg ctggtttcag attggtgaca aagtgcctgt gtttatgccg gatgcggcgt
    23161 taacgcctta tccggcctac aaaaccaatt aaattcaatg aattaaaaaa tgatgtagac
    23221 ctgatacata gcacaacagg ctattttgcg tttagcatca gtctcaaacc ggctccagat
    23281 agagccggtt ttggttttct gtcttaacgc accatgcacg ggcgcttgtt atcgaacgtc
    23341 cagccaggaa tcagatactg cattcccatc gcatcgtcac gcgcgccaag cccgtgtttc
    23401 tgatacagct catgggcttt catcacttga tccatatcga tttctacacc cagccccggt
    23461 ttttctggca cctgtaccag cccgcctttg atctcaaacg gttctttggt caggcgctga
    23521 ttgccttcct gccaaatcca gtgcgtatca atagcagtaa ttttacccgg tgcagcggcg
    23581 gcaacatggg taaacatcgc cagggaaata tcgaagtggt tgttagagtg tgaaccccag
    23641 gtcaggccaa attcatggca catttgcgcc acacgtaccg aaccttgcat tgtccagaaa
    23701 tgcggatccg ccagcgggat atcaacggat tgtaacgaga gcgtatggcc catctgccgc
    23761 cagtcggtgg cgatcatatt ggttgcggtt ggcaggccag ttgcgcgacg gaactctgcc
    23821 atcacttcac gcccggagaa accttgctcc gcaccacacg gatcttctgc ataagccagt
    23881 gaccctttca ggtatttacc aattttaatc gcttcgttca gcgaccaggc accgttagga
    23941 tcgagcgtaa tacgcgcctg cgggaaacgt ttcgccagtg ccacaataga ctcggcttct
    24001 tcttccccgg ccagtacacc gcccttcagt ttgaaatcgt tgaagccata tttttcatat
    24061 gccgcttccg ccaggcgcac caccgcatcc ggcgtcatcg cttcttcatg acgcaggcga
    24121 taccagtcgc atgagtcatc cggctggctt tgatacggca gcggtgtggc tttgcgatca
    24181 ccgacgaaga acagataacc gagcatttcg acttcgctac gctgttgacc atcgcccagc
    24241 agcgatgcca cgtttacccc cagatgctgc cccagcagat ccagcattgc cgcttctatc
    24301 ccggtaacta catgaatagt ggtacgcaaa tcaaatgtct gcaaaccgcg cccaccagca
    24361 tcacgatcgg caaaagtatt acgcaccagc gtcagaacgt ttttgtattc acccagcgtt
    24421 ttacctacca ccagcggaat cgcatcttcc agcgttttac ggattttctc gccgccggga
    24481 atttccccta cgccagtgtg accagaatta tctttgataa tcacaatatt acgcgtaaag
    24541 aacggtgcgt gtgcaccact cagattcatc agcatactgt catgacccgc caccgggatg
    24601 acctgcattt cagtaacaac aggcgtcgta aattgagaac tcatatttat gtcctttttc
    24661 agaatcaatg acggccgaaa acgggacgtt tacggtcaaa ggtccagccg gggatcaggt
    24721 actgcatcgg acctgcgtcg ttacgcgcac cgccaggcag acgtttatag gcctcatgtg
    24781 ccttttgtac ctgttcccag tccagttcca cgcccagacc gggcgcatca ggaacggcaa
    24841 tttttccgtt tttaatctcc agcggatttt tggtcaggcg acaatcgccc tcctgccaaa
    24901 tccagtgggt atcgatagcg gtaggattac ccggtgccgc cgcgcccaca tgggtaaaca
    24961 tcgccagaga gatatcgaaa tggttattag aatggcagcc ccaggtcagc ccccagtcgt
    25021 cgcaaagctg cgccacacgg actgcaccgg aaagcgtcca aaagtgcgga tcggcaagtg
    25081 gaatatctac cgcattgagc atcaccgcat gacccatttc gcgccagttg gtggcgatca
    25141 tgttagtcgc gacgggcaag ccggtcgccc gccgaaactc cgccataact tcacgaccgg
    25201 agaaaccctg ttctgcgccg catggatctt cggcataggt aagaacatca ttcagccctt
    25261 tgcatagaga aatggcttca tcaagtagcc aggcaccgtt gggatcaacg gtaatccgcg
    25321 catcagggaa gcgtttcttc aatgcacgaa cagtgtcgat ttcttgctcg ccaggtaaca
    25381 ccccgccctt aagtttgaaa tctttaaagc catagcgatc ctgtgaggct tccgccagac
    25441 gcacaacggc ttcgctgttc atcgctttct gatggcgcaa ctgataccac tcatggttgc
    25501 ccggcgtatt ttccagataa ggaagatcgg ttttggtccg atcaccgata taaaacagat
    25561 aaccgaggac ggtaatagtc tcgcgttgct tgcctggccc caacagttcg cagaccggaa
    25621 cattcagcgc cttacctagc aggtcaagca aggcggcttc cagcgccgcc acggcattaa
    25681 cgcgcaattc aaaagtccag gcacctttgc cgaaggtatc aaaatcggct gcctgattac
    25741 ctttatgcac ctgctgaacc actttattca ggcgcgcaac ttcctggccc agaaccatcg
    25801 gaatagcatc gaccagtgtt tgataaatca cctctccgcc cggcgcttca ccaatgccgg
    25861 tatgcccggc gttatcggtg agtaccacaa tattgcgggt gaaatatgcg ttatgtgccc
    25921 caccaatatt aagcaacatg ctatcgtgcc cggcgaccgg aatgactttc atatcagtaa
    25981 taacaggact ggattgtgtc gtcatcttat tgccccgcga caggtttcag ctcgatacgc
    26041 ttgatatcgc ccaccagcac caggtagctc agtaccgcga ttaaggcatg aacaccaaca
    26101 taaatcagcg ccccattaaa ggagccagtc gtgccaacga tataaccaat tgcgattggc
    26161 gtgacgatgc cagaaatgtt accgaacatg ttgaacaggc caccggaaag accgctgatc
    26221 tcttttggcg cggtatctgc cattactgcc caacccagcg ccccgatgcc cttaccgaag
    26281 aaggccagcg ccataaagcc gatgatcatc cactcaacgt tgacgtagtt gcagaacacc
    26341 atcaccatcg acagcaacat gcccattacg atcggtgttt tacgcgcaat gttcagcgat
    26401 cccgtgcggc gcatcagcca gtcggaaata atcccaccca gcacaccgcc gataaaaccg
    26461 caaaccgccg gaacggaagc cacaaagccc gctttcagaa tcgacatccc gcgtgcctgc
    26521 accagataaa ccgggaacca ggtaataaag aagtaagtca gggcgttgat acagtactga
    26581 ccgatataaa cgccgatcat catccgtgac ccgagcagct gtttgatctg cccccacttc
    26641 acgctgaacg gcactttaac tttagtgttt tgctgatcca tattgatcag cgcgccaccc
    26701 gcggcgatat actccagctc tttctgattt acccccggat gttgatttgg ctcgtggatg
    26761 actttcaacc agataaagct gatgacaatc cccagaccgc ccataaagaa gaagacgtgt
    26821 gaccagccca cttcatgcgt cagccagccc ataatcggcg cgaagatcac cgttgcgaag
    26881 tattgagcgg agttaaaaat cgacaccgcc gttccccttt cctgcgccgg aaaccaggcc
    26941 gcaacaatgc gactgttgcc ggggaaagat ggcgcttcag caagcccgac caggaagcgc
    27001 agagtaaaca gggcaacgat aatgccgaat ccactaaaga tatcgacgaa gccttgcagc
    27061 aaggtaaaca tcgaccagat aaagatcgac cagaagtaga cgcgttttga accaaaacgg
    27121 tccagcaacc agccaccagg gatctgcccg ataacataag cccatgagaa ggcagagaac
    27181 acatagccca ttcccacggg atcaaggccg atatctttgg ccatttccga accggcaata
    27241 gaaagcgtag cgcggtcgcc gtagttgaag gatgtgacga taaacaacat caccactatc
    27301 cagtaacgag cattcgtgcg tttttccaca ctgctcgcag cctgacttaa agaactcatt
    27361 gttgcactcc tgaaaattcg cgttagccac gctcactctg gactgcgaca tagccaggaa
    27421 atcagaggtg acgtagggtg ttttttgccg tttttatagg tcgttcgccg aatacggcgc
    27481 gtgtttatat ctggcaatag cagtataaaa agcgcgccat agcggctcac cgtgcaacaa
    27541 cacaacatta atgcgttcaa tgaggcccga ttttggcatt agcccaggac ggtggaatcc
    27601 acttcacgga aatgaaaaca agaacgagaa aggaagggtt aaaacgaaga aataaagaag
    27661 agtatgaaat ggatcgcttg actccaggca aacgccagta aaaatccgcg ctatgaagca
    27721 gtttttactg gcatttgcct gaaaagattc gattcagcac cgctaaaacg acatttaccg
    27781 ctcgctgaac atatcaggac aacagcgtgc cccactgttc gacccacgga tttgattccg
    27841 tttccggttc cgggttctca ctggcgtcaa tcaacagtat ttcgccaacc cgctgagcgc
    27901 tctgttcctg caacaaggca tcgaactgtt tgccgccatt gcagaaattt acataactgc
    27961 tgtcgccgag cgcaatcacg ccataacgca gattcggctg gaagcccaga ctatctttga
    28021 ttccctgaaa gagcggcaca atgctatcag gaaggtcgcc ctgcccggtc gtggacgtaa
    28081 ccaccagaac atacttatcc tgataaggca gccagtcgct taattcagga tcttcaaata
    28141 ccgttgcttt gtggccctgt gcggtcagaa ttgcttctgc ttcttccgcc actaacagcg
    28201 aatttccgta catggtgccg acaaaaatac caatttccgc cataccgttt tctccctgga
    28261 ttaggaactt atctctccat cctgacccga tggcgcactg aactcaaccc tttcattttc
    28321 aggaagcaga ccgcgccagc caaattgtga taacgcctgc atccaggtgt cgtccaggcc
    28381 cgcgtgaata gtcagcggct cgccagtaaa aggatgcgtc aatgacaact gactggcatg
    28441 tagcattaac cgctggaggc caaaatgctc agcaccgctg cgattctggc gtaaatcgcc
    28501 atgtttgcta tcgccaataa tcggatgacg caaatgggca agatgtcggc gaagctgatg
    28561 tttgcgtccg gttttcggct ccagttccac caggccgtag cgcgtggtcg ggtaacgtcc
    28621 ggtcgctacc ggcatttcta cggtcgccag accgcgataa tgcgtcactg ctggctgcga
    28681 gcctttatct tcgcgggcaa atttatcagc gattttgtcc agttcttcca ccagtggata
    28741 atccagaact gcttcttcca tcaaccagcc gcgcacaatc gcatggtaac gtttctggat
    28801 ttggtgctgt tcaaactgtt gtgccagcag ccgtccggcc tcgctggata atcccatcaa
    28861 caacacacca gaagtgggtc ggtccagacg atgagcggta aaaacgtgct ggccaatctg
    28921 atcacgcacg gtttgcatga ccactacttt ctcgtcgcga tccagccagc tgcggtgaac
    28981 cagccagccg gagggtttat ttaccgcaac cagccattca tcctgataga gtatttccag
    29041 cattagctcg catcatccgc aaaaagagca tccagttttt ccagctcagc cagaataagc
    29101 gcgcgttgcg gatggtccgt cgccagcgcc atttcataat agggtgcaac ggcaaaagcg
    29161 cccggtaacg gctgtttgtt atctaacaaa tcgtgcattc gcgggatcag cacccactgc
    29221 aaccactcca gtggttccat ggtgtccata aagaacggtt gggtactatt aaattgatgc
    29281 ggctggggtt catcgtttcg ccagtgctga tgttcacgca gtaacgcttc aagcgcctgc
    29341 aactggagac gaacgcggtc atgagtggtc ataaagcaac ctcaataaga aaaactgccg
    29401 cgaaggatag caggaataaa aaaagggagc actgtattca cagcgctccc ggttcgtttc
    29461 gcagcattcc agctactttt gttgctccct gctcatcctt gacaactttt cctctggcct
    29521 tgcggccaat cgttcatcct gaactattgc ttcctgctca caccaccccg atgtgatact
    29581 tcatcctgaa gtgtccctgg ccttcctgac ccaccgaatc atcctgaccg gttctcattc
    29641 tccatcctgg aggtgtcctt taacgcgtcc tgcgtcatcc tcttcgcttc atccagaagc
    29701 ctttccctga aacaccatcc tggtgtgtcc tgcagaagtg tcatcatcct gatgttcgct
    29761 tcgttgtctg actccctgtc gacgaagata gaatcgtctt tttcgcgaag tcttacaagg
    29821 cgcttacaga caatgcctta aggaaaattt catatgaaag ttaagaatta acaatcaaca
    29881 ttttgatttt aaatgaaaat aacgattctt atctttttaa gtattcgctt atttcctggc
    29941 gatctcctac agagtctgta agagatctct cacaaccaga tggggtattt ggattacaga
    30001 agaggcttaa gttgattaag gaattccgca agatttgagg ctaaatgcgt ccgtttacgt
    30061 gttcccagcg tctctttgca cacttcaccg gagagattac acaccgagat gacctctaac
    30121 tcttcttcca gcgtcgcgat aaatagcgtg ggcggcagtt ttagcctttt ctgagtcacc
    30181 agatgaccaa tcagattttc ctgcacccga cggaagtcgt cttcactcca ggtttgcagc
    30241 aatgtcagtt tgatatcgcc aaactgcgcg tgcatatccc cggcaaactg agtcgtataa
    30301 aaggtatgaa ttgtgggttg tatcacaata tcaaaagcgc gttcaaccgc gtttacattt
    30361 tgttccccgg taaacggctg gggttgccag tatacggcat cttcggtagt tgaaataatg
    30421 cacggtgaag gaacaccgta cagttcctca cttaacggcc agcttttatg ctcttcatgc
    30481 cacgcatcgc agtagcgtgc agtaaaatct ttcagggctt gtgcggtcaa atcgtccact
    30541 aatttctctc ttcacttaaa ccagatacac ttgtcgttta gtttatctgg tttatgacgg
    30601 tgaaacatgt cttcttatgc aaaccatcag gcacttgcgg gcctgactct tggaaaatca
    30661 accgattacc gggataccta tgacgccagc ctactgcaag gcgttccacg cagcctgaat
    30721 cgcgacccgc tgggtctgaa agcggataac ctgccttttc acggtacgga tatctggacg
    30781 ctgtatgaac tttcctggct gaatgcgaaa ggtttgccgc aggtcgctgt cggtcatgtt
    30841 gaatttgatt acaccagcgt aaatctgatt gagtcgaaga gttttaagct ctatctcaac
    30901 agttttaacc agacgcgttt taataactgg gatgaggtgc gccagacgct ggagcgcgac
    30961 ttaagcactt gcgcccaggg taaggttagc gtggcgttat atcgtcttga tgaactggaa
    31021 ggccagccga taggtcattt caatggcacc tgcattgatg accaggatat cactatcgat
    31081 aactatgaat tcactactga ctatctggag aatgccacca gcggtgaaaa agtagtggaa
    31141 gagacgcttg tcagccacct gctgaaatca aactgcctga tcacccatca accagattgg
    31201 ggttcgatcc aaattcagta tcgtggacgc caaattgaca gagaaaaact gctgcgttac
    31261 ctggtctcat tccgtcatca caacgagttc cacgaacagt gcgtggaacg catctttaat
    31321 gacctgttac gcttctgcca gccagagaaa ttaagcgttt acgcacgtta tacccgtcgt
    31381 ggcggtctgg acattaaccc gtggcgcagt aatagcgatt ttgtcccatc gaccacaaga
    31441 ctggttcggc aataaatttt ttctcaattt tgcgtgctgg attcacgcag aaggttgtga
    31501 aaggtcatca ggcagggcta ttgtaatcaa agggaatgac gatattcgtc ccataaggag
    31561 tttttcttga ttacacatat tagcccgctt ggctccatgg atatgttgtc gcagctggaa
    31621 gtggatatgc ttaaacgcac cgccagcagc gacctctatc aactgtttcg caactgttca
    31681 cttgccgtac tgaactccgg tagtttgacc gataacagca aagaattgct gtctcgtttt
    31741 gaaaatttcg atattaacgt cctgcgccgt gaacgcggcg taaagctgga actgattaat
    31801 cccccggaag aggcttttgt cgatgggcga attattcgcg ctttgcaggc caacttgttc
    31861 gcggttctgc gagacattct cttcgtttac gggcaaatcc ataataccgt tcgttttccc
    31921 aacctgaatc tcgacaactc cgtccacatc actaacctgg tcttttccat cttgcgtaac
    31981 gctcgcgcgc tgcatgtggg tgaagcgcca aatatggtgg tctgctgggg cggtcactca
    32041 attaacgaaa acgagtattt gtatgcccgt cgcgtcggaa accagctggg cctgcgtgag
    32101 ctgaatatct gcaccggctg tggtccggga gcgatggaag cgccgatgaa aggtgctgcg
    32161 gtcggacacg cgcagcagcg ttacaaagac agtcgtttta ttggtatgac agagccgtcg
    32221 attatcgccg ctgaaccgcc taacccgctg gtcaacgaat tgatcatcat gccagatatc
    32281 gaaaaacgtc tggaagcgtt tgtccgtatc gctcacggta tcattatctt ccctggcggt
    32341 gtgggtacgg cagaagagtt gctgtatttg ctgggaattt taatgaaccc ggccaacaaa
    32401 gatcaggttt taccattgat cctcaccggc ccgaaagaga gcgccgacta cttccgcgta
    32461 ctggacgagt ttgtcgtaca tacgctgggt gaaaacgcgc gccgccatta ccgcatcatc
    32521 attgatgacg ccgctgaagt cgctcgtcag atgaaaaaat cgatgccgct ggtgaaagaa
    32581 aatcgccgcg acactggtga cgcttacagc tttaactggt caatgcgcat tgcaccagat
    32641 ttgcaaatgc cgtttgagcc gtctcacgag aatatggcta atctgaagct ttacccggat
    32701 caacctgttg aagtgctggc tgccgacctg cgccgtgcgt tctccggtat tgtggcgggt
    32761 aacgtaaaag aagtcggtat tcgcgccatt gaagagtttg gtccttacaa aatcaacggc
    32821 gataaagaga ttatgcgtcg tatggacgac ctgctacagg gttttgttgc ccagcatcgt
    32881 atgaagttgc caggctcagc ctacatccct tgctacgaaa tctgcacgta atctcccctc
    32941 ccccggtgac gtttgttgcc gggttttctc gtttttggtc acttactcat caactcattt
    33001 catttgttat atgaatgttt cttaccaccc tcacgcgaca aatatcatca cagttaatat
    33061 gtcatacaat ttatattgca acgc
//
LOCUS       DAMZET010000030        32564 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.030, whole genome shotgun sequence.
ACCESSION   DAMZET010000030 DAMZET010000000
VERSION     DAMZET010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.030"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..485)
                     /gene="epmC"
                     /locus_tag="POB34_001567"
     CDS             complement(<1..485)
                     /gene="epmC"
                     /locus_tag="POB34_001567"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HDI5918543.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     L"
     gene            complement(485..1294)
                     /locus_tag="POB34_001568"
     CDS             complement(485..1294)
                     /locus_tag="POB34_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDI5918544.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(1294..2118)
                     /gene="mepA"
                     /locus_tag="POB34_001569"
     CDS             complement(1294..2118)
                     /gene="mepA"
                     /locus_tag="POB34_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HDI5918545.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(2122..3207)
                     /gene="aroC"
                     /locus_tag="POB34_001570"
     CDS             complement(2122..3207)
                     /gene="aroC"
                     /locus_tag="POB34_001570"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDI5918546.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(3242..4174)
                     /gene="prmB"
                     /locus_tag="POB34_001571"
     CDS             complement(3242..4174)
                     /gene="prmB"
                     /locus_tag="POB34_001571"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDI5918547.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            4340..4891
                     /gene="smrB"
                     /locus_tag="POB34_001572"
     CDS             4340..4891
                     /gene="smrB"
                     /locus_tag="POB34_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HDI5918548.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(5013..5885)
                     /locus_tag="POB34_001573"
     CDS             complement(5013..5885)
                     /locus_tag="POB34_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HDI5918549.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRTVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(5872..6396)
                     /locus_tag="POB34_001574"
     CDS             complement(5872..6396)
                     /locus_tag="POB34_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI5918550.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(6393..6863)
                     /locus_tag="POB34_001575"
     CDS             complement(6393..6863)
                     /locus_tag="POB34_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI5918551.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            complement(6860..7408)
                     /locus_tag="POB34_001576"
     CDS             complement(6860..7408)
                     /locus_tag="POB34_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI5918552.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(7383..8135)
                     /locus_tag="POB34_001577"
     CDS             complement(7383..8135)
                     /locus_tag="POB34_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDI5918553.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPWQYKV
                     TLTRNGQEFTVNNPTPYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNSCQVDEEQSRKG"
     gene            complement(8155..10800)
                     /gene="yfcU"
                     /locus_tag="POB34_001578"
     CDS             complement(8155..10800)
                     /gene="yfcU"
                     /locus_tag="POB34_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HDI5918554.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEEFSGDDTQKKWEWSRYYAWRALPSLKAKLALGEDYLNSDIFDGFNYVG
                     GSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDS
                     VSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSG
                     AEASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYG
                     KGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKE
                     MYTATYNQNFRDASVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNMSVSLTGY
                     RYEYDNRADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVG
                     TSDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEA
                     TQSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDD
                     DGNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDD
                     IKPVIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(10882..11445)
                     /locus_tag="POB34_001579"
     CDS             complement(10882..11445)
                     /locus_tag="POB34_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDI5918555.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(12129..12614)
                     /gene="sixA"
                     /locus_tag="POB34_001580"
     CDS             complement(12129..12614)
                     /gene="sixA"
                     /locus_tag="POB34_001580"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HDI5918556.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(12817..14961)
                     /gene="fadJ"
                     /locus_tag="POB34_001581"
     CDS             complement(12817..14961)
                     /gene="fadJ"
                     /locus_tag="POB34_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HDI5918557.1"
                     /translation="MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPAIERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(14961..16271)
                     /gene="fadI"
                     /locus_tag="POB34_001582"
     CDS             complement(14961..16271)
                     /gene="fadI"
                     /locus_tag="POB34_001582"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HDI5918558.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKIV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRVKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(16452..16736)
                     /locus_tag="POB34_001583"
     CDS             complement(16452..16736)
                     /locus_tag="POB34_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDI5918559.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFASRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            17108..18448
                     /gene="fadL"
                     /locus_tag="POB34_001584"
     CDS             17108..18448
                     /gene="fadL"
                     /locus_tag="POB34_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HDI5918560.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            18816..19874
                     /locus_tag="POB34_001585"
     CDS             18816..19874
                     /locus_tag="POB34_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918561.1"
                     /translation="MLPSISINNTSATYPESINENNNDEVNGLVQELKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGNSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSEKARNASAWFESSKAIMAESFATGSDHQVINELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISNLFANKVDVDHPENKHKDFKDLEDNVEKRLQNRQAK
                     YQNEINQLSAPGVNFDDI"
     gene            complement(20056..20811)
                     /gene="mlaA"
                     /locus_tag="POB34_001586"
     CDS             complement(20056..20811)
                     /gene="mlaA"
                     /locus_tag="POB34_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HDI5918562.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            21106..22038
                     /locus_tag="POB34_001587"
     CDS             21106..22038
                     /locus_tag="POB34_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDI5918563.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            22114..22188
                     /locus_tag="POB34_001588"
     tRNA            22114..22188
                     /locus_tag="POB34_001588"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22147..22149,aa:Arg,seq:cct)
     gene            complement(22630..23877)
                     /locus_tag="POB34_001589"
                     /pseudo
     CDS             complement(22630..23877)
                     /locus_tag="POB34_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
     gene            complement(23949..24863)
                     /locus_tag="POB34_001590"
     CDS             complement(23949..24863)
                     /locus_tag="POB34_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HDI5918564.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGI
                     SGFIGRVGDDPFGALMQRMLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDQDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            25079..26512
                     /gene="cscA"
                     /locus_tag="POB34_001591"
     CDS             25079..26512
                     /gene="cscA"
                     /locus_tag="POB34_001591"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDI5918565.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(26520..27578)
                     /locus_tag="POB34_001592"
     CDS             complement(26520..27578)
                     /locus_tag="POB34_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI5918566.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            27758..27976
                     /locus_tag="POB34_001593"
                     /pseudo
     CDS             27758..27976
                     /locus_tag="POB34_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            27994..29322
                     /gene="dsdA"
                     /locus_tag="POB34_001594"
     CDS             27994..29322
                     /gene="dsdA"
                     /locus_tag="POB34_001594"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HDI5918567.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(29430..30968)
                     /gene="emrY"
                     /locus_tag="POB34_001595"
     CDS             complement(29430..30968)
                     /gene="emrY"
                     /locus_tag="POB34_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HDI5918568.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGIG"
     gene            complement(30968..32131)
                     /gene="emrK"
                     /locus_tag="POB34_001596"
     CDS             complement(30968..32131)
                     /gene="emrK"
                     /locus_tag="POB34_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HDI5918569.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQF"
BASE COUNT         8754 a         8118 c         7544 g         8148 t
ORIGIN      
        1 tgtagtgctt taatgaagcg tgccgggcgt tcaggaatgc cgtttgctag gtaatccatc
       61 acctgcgcat gtacccgccg ttgaaacacc acacgatccg gttcgaagtc gccttccaga
      121 ttgtcgcaac tgacgttaaa cggatatccc gccgccacac agaacaacca gtcgagtgcc
      181 tgcggcttca cttcaacatc ttcaaactgg ctttgcgttt gggcatcacg cccatccggg
      241 caataccagt agccgaaatc aaccagctca cggcgcgctt ttccggcaat acaccagtgc
      301 gaaatctcat gaattgcgct ggcataaaag ccgtgggcaa agacaattcg gttatacggt
      361 acttccgcat cagcaggaag atagatcggt tcgtcgtcgc ctttaatcag acgggtatta
      421 aattcatcgg caaagcagct attaaaaatt tcaatcaact gctcatagtg gtgtgtactg
      481 ttcattagtt catccccaac cagtggagga tctcctgtcc gtggctatcg taaagtaatt
      541 tggcactcat caccgccgag acgataacaa tcatcgggcg gatcagcttt tgtcctttgc
      601 tcaacaccag ccgtgaaccc atgcgcgcgc cgaggaactg ccccaccagc atgacaaagc
      661 ccgtcgccca aatcactttg ccgccgagaa taaacagcag cagaccgcca atgtttgacg
      721 ttgcgttgag taatttggcg tgagccgtgg ctttggcgag gttgaatccg cacagcgtaa
      781 cgaaggccag cgcgtaaaac gatccggcag ccgggccaaa gaatccgtca taaaaaccga
      841 cgcagccacc ggcaattaac gcgaagggta aaccgtacat ccggcgctgg cgatcttctt
      901 cacccagctt tggcatcagc aagaaataga ggccgataca aatcaccaga atgggcaaaa
      961 tctgccgtaa gacatcagcc tgaacgtact gtaccaacag tgcgccgctc attgagccga
     1021 caaaggtcat ggcaatattg agtttctgat cgcttaaact aaccactttg cggcgaataa
     1081 agtagatagt ggcggaaata gagccgccgc aggcttgcag tttattggtt gccagcgcat
     1141 tagcgggaga catccccgct gccatcaatg ccggaatggt gagtaaccca ccgccaccgg
     1201 caatcgagtc gataaatccc gccagcatgg cgacaaaaaa gagaactccc agcaacagcg
     1261 gggaaaccat aaacaggcta ttaaacgttt ccattagatc acgtgctcat ccagtagcgc
     1321 ctggcaggaa ggcggcaacg gaggcggtgt cttcttctca ggctttgttg ttcccggttt
     1381 tggaggtgca aaccagcttt gcagttctgc cccgcaacca tcgcctggtg gcggtaaagg
     1441 ttgatcttca cactccagac tatcggcagg acaacgtaat cgtacatgca tatgcgcgcg
     1501 atgctggaac cagggtcgca ctttgcgcaa ccagtcgcga tcggtgcccg catcaaggca
     1561 aagttgttgt ttaatcgccg gattaacaaa aatgcgtgtg acgtctttgt cctgggcggc
     1621 gagtttgatc aagctgaaaa tttctggctt ccacagcgtg gagacaacgt gtttaccgtc
     1681 gcgggaaact aagtccagtg cttgcgggcg caagagctgc gcggaggtcc agcgagtttt
     1741 cggcagttgc agaaagatat cgacatccag tccggtctgg tggctggcat gaccgccgtt
     1801 gaaacgccca ccagcgggca tccccatatc gccaatcagc accgtaccca tgcccagatt
     1861 gctcacctgg ctactcagac gctggataaa catcaccaga tccgggtgac cgaaatagcg
     1921 acgctgatcg gtacgcatga cctgataatg ttcggactgt atcggcagcg tgtcagcacc
     1981 gacgatacag ccattagaaa aactgcctat cgattgtgcg ctacccggca caggttgggt
     2041 tattttttgc cacggcgtcg ctgccaggct agcgctactg gcaagcagag ccagcagcgc
     2101 aatcgcggtt ttgttcattt tttaccagcg tggaatatca gtcttcacat cggcattttg
     2161 cgcccgctgc cgtaacaggt gatccattaa aacgatcgcc agcatcgctt cagcgatcgg
     2221 cactgcacgg atcccgacac agggatcgtg acggcctttg gtgatcatct caacttcttc
     2281 gccaaagcgg ttaatagtgc gccccggcac ggtaatgctg gaggtcggtt tcagcgccat
     2341 atgagcaatg atttgctgcc cgctgctgat accgccgaga atgccgcccg catggttgct
     2401 ctggaaaccg tctttggtga tttcgtcgcg gttctggctg ccgcgcagcg ccactacgtc
     2461 aaaaccgtcg ccaatttcca cgcctttcac cgcgttgatg ctcatcagcg catgggcgat
     2521 gtcggcatcc aggcggtcaa agaccggctc gccaagtccg gcggggacac cactggcaac
     2581 aacggtgact ttcgcgccga tggagtcgcc ctcttttttc agcgcgcgca tcagttcatc
     2641 taacgcgtcg attttgtcgg ggtccgggca gaagaacgga ttttgttcaa ccagcgacca
     2701 gtctttgatt tccagcggaa tgtcgcccat ctgggtcaag cagccgcgga tttcaatacc
     2761 aaatttctcg gcgagatatt ttttggcaat cgcccctgcc gccacgcgca tggcggtttc
     2821 gcgggcggaa gaacgtccac cgccgcgata atcgcgcaga ccgtattttt gttcgtaggt
     2881 gtaatcagca tggcctgggc ggaaaacgtc tttaattgcg ctgtaatcct gagagcgctg
     2941 gtcagtgttt tcgatcaaca agccaatgct ggtgccagta gtaacgcctt caaaaacacc
     3001 ggagagaatt ttgacctgat ccggctcgcg gcgctgggtg gtatagcgcg atgtcccagg
     3061 gcgacgacgg tcgaggtcat gttgcaggtc cgcttccgtc agcggaatgc ctggcggaac
     3121 accatcgacg atgcagccga gcgccagccc atgcgattcg ccgaaggtgg ttacgcgaaa
     3181 gagttgtcca attgtgtttc cagccatcac ggctccgtta ttgttgtgtt tgcgtgttta
     3241 cttaatcttt ataaatcgcg aaatattcac gggccgccag cagctgctct ttggtgagca
     3301 taaacacacc atcgccgccg ttatcaaact ccagccaggt gaacggaaca tccggatatt
     3361 gttccataag atgtaccatg ctgttgccga cttcacaaat caacacgcca tcatcagcaa
     3421 ggtaatctgc cgcgtttcca agaatgcgac gtgtcagttt aaggccgtcg gtgccagatg
     3481 ccaggcccag ttccggctcg tgacggtatt cgtttggcag gtcggacata tcttccgcgt
     3541 cgacatacgg cgggttagtg acaatcagat cgtattgcac tttcggcaaa tcacggaaca
     3601 gatcggaacg aatcggaatg acgttgtgga tcagaccgtg ttcttcgatg ttctgttcgg
     3661 caaccgccag cgcgtcggga gagatatcta ccgcgtcgac ttccgcttcc gggaaggcat
     3721 aagcacaggc aatggcgatg cagccgctac cggtacacat atctaaaata tgctgcggtt
     3781 gcttgctgat gagtccggca aatttattgt tgatcagttc accaatcggc gagcgcggca
     3841 ccagcacgcg ttcatcgacg taaaattcat gaccgcagaa ccaggctttg ttggtcaggt
     3901 aagccaccgg aatgcgctcg ttgacgcggc ggatcacgcg ttcaacaata cggtgttttt
     3961 cgctggaggt cagacgcgcg gtgcgcatat cttccgggat atccagcggc aggtagagcg
     4021 atggtaacac cagctgtacg gcttcatccc acgggttatc ggtgccgtga ccgtaccaga
     4081 tatttgccgc gctgaagcgg cttaccgacc agcgcaacat gtcctgaatg gtttgcagct
     4141 catttactgc ttcatcaacg aaaattttat ccacgtattc ctccagggca tgatcgcaat
     4201 aatttcggcg gctagtttgc catgaagatg acgataaatc agcattcacg cgcggtgagt
     4261 gaggaaaaat acgtttaaaa cgatcgattg tgctacgagt cgggtaaact gtaggaaaat
     4321 tagaaataga gacagataaa tgaaaaagaa aacaacactt agcgaggagg accaggctct
     4381 gtttcgccag ttgatggcgg ggactcgcaa gattaagcag gacacgattg tccaccgacc
     4441 gcagcgtaaa aaaatcagcg aagtgccggt gaaacgcttg atccaggagc aggctgatgc
     4501 cagccattat ttctccgatg agtttcagcc gttattaaat accgaaggtc cggtgaaata
     4561 tgttcgcccg gatgtcagcc attttgaggc gaagaaactg cgccgcggcg attattcacc
     4621 ggaattgttt ttggatttac acggtctgac gcagctgcag gccaagcagg aactgggcgc
     4681 gttgattgcc gcctgtcgcc gcgaacatgt gttttgtgcc tgcgtgatgc atgggcacgg
     4741 gaagcatatt ttgaagcaac aaacaccgct gtggctggca caacatccgc atgtgatggc
     4801 atttcaccag gcaccgaaag agtacggcgg tgatgcggct ttgttggtgt tgattgaagt
     4861 ggaagagtgg ttgccgccgg agttgccctg aatgctggca gcaaatgccg gagcagcgta
     4921 aacgccttat ccggcctaca aatatgtgat aattcaataa attgcaattc acttcgtagg
     4981 cctgataaga cgcatcagag caatactacg aactacagtt cattcaatac atactgaaaa
     5041 cgacacgtcg aatcaatcgg ttgcccacta ctggtattca cattcaccac aatatcgtgt
     5101 agtactttat ccgttaccgg atgacgagtt ggcgtaaccg ttaccgttga tttatcacaa
     5161 ccgtaagagg taaaggttaa caattctgat accgtgcggc agtatgctcc tctgccatca
     5221 ttatcagaaa aattactgcc atcacagagt ctgccgcctt ccatcaagta ggtcgaaagc
     5281 gtggtggtca ctgttgaccc cgtggactga attcgtaacg ccttattatg gctgacataa
     5341 ttttcactcc aggagatatc gtatgaagcg accggtatcg ttgggcatat tgtgttatgc
     5401 actgtagggg ccgtatagct tccaaatccg ggtaaatctg tataggaata aaaggatgcg
     5461 atccaagggc aaccataatc atcagatgca acggtaattt taaacgtcat atatgcacaa
     5521 cgaaaaattt tcgtatctat gccagggcaa tttgagttat taattgtatc aaagtcagtc
     5581 agtaccaaag ttggagctga acccgaccca gaccaggaaa taacccgcaa cgtatcgcct
     5641 ttatacgcaa aagaatcata tttcccatat gcaacctcct ttggcgttat caccgtaaca
     5701 ttgaaaacac tgctcgctac agcaccggtt accttgtcca tggcataata aactctcaca
     5761 ctgtcaaaac tcgcactcat aagtttatct ccagcataac tcttagatgc gccgacaaca
     5821 ctcagaacga gcagcattag gctcgcgaaa attatggtcc attttttcat ttcagaaata
     5881 ctcaaaggtt gccgtcactg tggcactaaa gtctccggac gttacatctc gcccgctctt
     5941 tgcctgtaac caggtattaa aatttaggct gttatttccc tgattcagcg ggagcgtgac
     6001 gcctgttgta ttgttgattg gcaccgatgt gcctgccgca gtttcaatac caatcccagc
     6061 gccggatgct gatgacgagg agtcaaaggc cagaaagccg ggtagagcac tatcttctgt
     6121 tcctgtcaac gttactttga cattaaacaa tgtagaacta tgacaatctt tcaattgaaa
     6181 caccaatggc actcgctctg actgcccaag gttcattaaa tcgctggcgg caatggcagg
     6241 aaaatgtact tctgccaact ggctaccatt aatcactggc gtacacgatt tactgattaa
     6301 attgccggta aagtgcaaat tatctcctgc tattgtggtt ccactgccca gcaacatcag
     6361 gctactaata aaaaacaacg tacgcttttt cttcattggt aatctacctg taacgtggcc
     6421 caggcctcaa agtccgcctc tggcagaacg acaccactct tttttactgg cacagccgtt
     6481 aacaccggta aatcggtttc attcaccaca agtggcgtat tgatcgtaaa actctgccct
     6541 gcctgtttga gctgaatgcc taaaccagtg atattgctgc ttactgcagc attatcaaaa
     6601 tcgcttggtg tgccactcca gctcaaggtc atttccatta atgggtcgcg aaccgccgta
     6661 tcgcaggtaa tggaatacgg cacggtttgc tcataattgc tgccattaat gtcatcaata
     6721 agcacatcct gaaaagttat ttcaatggtt tgagcgttgc taatcgtgca gttaggtggc
     6781 tgcactaaat aaccgtgaaa agagacgttt gagagtgcca tcgagcaaaa gccccacagc
     6841 aaaataagcg agatccgttt cattggtaat ccaccacaat tgtggcactg gcgttaaatt
     6901 cagctgccgc taacgttgta ccactctgtt tcaccggcac agcttccaga acgggaactc
     6961 ccgaggaaaa attaaatggc aaccatgagc cagaggttaa gtcgagtatt gtgtgatcac
     7021 tacttttctg aacccgaata ccaaacccag gaatccccgt gctgataact gtttcaccgt
     7081 taatcacgac cgttgtcgcc tgcatctgta gacgcaggtc atccattgcc agattgttac
     7141 aaacaagcga gtagtcaatg ggacgtttat aatcaacccc attgatctta ttaatgaata
     7201 cattaccaaa ctcaactgcc gcaccattta ccgtacaagg aggaggggca tcgacaagca
     7261 cccgtaaatt catacttact gacttactcg ctgcaactgc aaagccgcta ccaacacaac
     7321 atatcagtaa cccagttacc gcccagacag aacaagatcc tttcatactg gctctcccgg
     7381 cctcagcctt tgcggctctg ctcttcatcg acctggcaac tgttaccgtt gcaagtgaaa
     7441 aagagcggca tccgagcgcc atagtcgttg acataggtca gtaccggcac gctacccatc
     7501 ttcacattca gcggtgcaga ggttttcggc gacatcacta ttggcgagaa accggctgcc
     7561 ggattgccat ttttctgtgt gctggcatta ctgaaaatga cgtaatacgg tgtcgggtta
     7621 ttgacggtaa attcctgacc gttgcgggtt aaagtaacct tatattgcca ggggtttttc
     7681 atgctgacgt tctccagcgc cttcggtcgc cagaacaact taatacgcgt ttgtagtgct
     7741 atttgcagcg tgttggcttt gtttgatttt ggcgggatct cacgcacgtt gaagtagaac
     7801 aggctttcac gatcggcagg caatttgttg atatccggca ttccctgcac ttttacctgc
     7861 ccgttcatca ttgaatcgat gcgttgcacc ggtggcagaa cagttaacgg tgaactgatc
     7921 ttgttaccct tttcatcttc gatccagctt tgggccagat aaggcagttt cggatcgtta
     7981 ttgcgcaacg tcacgctgat tgatttgtcg ctttcattaa atatcaaacg ggtacgatct
     8041 ggcgttacag aagcctgggc agacaaactg gcagcgctaa gcagtaacgc tagcgtcgta
     8101 gcacccaatt tggcagagca aagtaaatca gacatggatc attccttaat cacgttattg
     8161 gttagctgaa acagaaacag ggggattggt gggtgtcacc tgttgcgtct gctcctgaat
     8221 gacaggcttt atgtcatcag gaacaaccgg cgcgacgttg cctttatgct ggcatggcag
     8281 taacaagccg ttaaacagat cggcaggcag cgggtccggc aggttgatat cgcaatgcgc
     8341 aacaccactc cagaacacac tcatgtgttc gccaggtttc accccagcca gataaacatt
     8401 gccgtcatca tcgacaaggc ccactgtctg ctcgttatca ttttttactt ctgcgccaaa
     8461 cggaggatgg ctgccgtctt gtaaacgcag caccgccatc gctttttgac cactgatgac
     8521 ggcaaatttg cggtagccaa tcgccccttc agttagcgtg gcttgtacca ccgactgggt
     8581 tgcttcggcg ttttccggca gtttgttgag gtcgatatac gcctgattgc ggtaatagtt
     8641 attgacgtca gaaacgacgg ctttaccaaa catattggta taaacagcag caccgttacc
     8701 ttctaccgga acatcggcaa cgccatcggc atcaatcaac aagcgtgtcc cgcccatatt
     8761 ctgggtacgg tgaagtgcgc caccgtgggc agtaagcgtt gcgccgccct gtaacgacaa
     8821 gcccgcagag gtgtattgcc cttcatggta gttcgcgctg aggtcaacct gcgccagcga
     8881 accatcatgg ctgtaatagc cgtcaacgct ggtgtgtttg tcactggtgc cgacgttcaa
     8941 ctgatagtga gtcgcgtcat cgacacggct gaaataaccg acctgactgc tgtccgtccc
     9001 actgccatag ttgccgttat agctaacggt actgttgtcg ccccacggca tactgagcga
     9061 aatgtacatg cccttatccg cccggttgtc atactcgtag cggtagccag tcagggaaac
     9121 gctcatatta cgaatgctgc ccatattgaa atagtgggag agcatgatgt tgtagtttgt
     9181 ctgctcctca cgatcccagt aagtatggcg ggtatagttg agataaaccg aaacactcgc
     9241 atcgcggaag ttctggttat aagtggcggt gtacatctct ttgtcgttgc ccgtgcgtac
     9301 cattccgctg tcactggcat ccagatactc gctcatggtc ataaagttct cttccgagaa
     9361 gcgatatcca gcaaaagtaa cgcggctgtt gagctggtca aagtctttgg aataactcac
     9421 acggaaggag ttaccgtcca gcgaaccttt gccataagcg gtgtctttat ccagtttggt
     9481 gtgcgagtga gtaacatcaa acgcgaccgc gccgaacgta gacaaatcgc gaccaacgcc
     9541 aagtgcggca gattggtagt tttcatcccc cagcgcgcca ccatagagcg accaaccatt
     9601 agcgatcccc caggaagctt cagcaccaga gaaaaattcg ccttcaacat gatgccccca
     9661 ctcttgcgga cgacccatca tgatcttata gcgcacctga cctggacgag tgaggtatgg
     9721 catcgaggcg gtgctgatgt catattcctg cacctggccg ttctgttctt caatgcgaat
     9781 atgcaacgta ccggagacag aatcaccaag atcctgaata cgaaacggtc cagccggaac
     9841 ctgcgtttcg taaatcacac gccccatctg gctgacggtc acttttgctg tggtgtgtgc
     9901 cacgccggaa atgtctggcg cgtagccgcg caggttggga ggcaacattt gatcgtcagt
     9961 gctgacactg ccaccaacat aattaaaacc gtcgaaaata tcggaattga ggtaatcttc
    10021 gcccagcgcc agtttggctt ttaatgacgg taacgcccgc caggcataat agcgactcca
    10081 ctcccatttt ttttgtgtgt catcgccgct aaattcctcg tcatcatcat tactgcgggt
    10141 atgttgatag ttggtctgcc agtcagcgcg catacgccat ggccccaggt taaccccgac
    10201 cgtcccgttg ccgctgatct cgttactatc atcaccgcca ttttcttcgt gccgggtttg
    10261 tgcgttgatg ctgtagtccg cgacgatccc ggagatgccg tcatcccaac gtgaaggcgg
    10321 atcccagtcg ggataagtat attcgaggta agcctgtggc agtgaaatga ctaatgcgga
    10381 ctggcttaaa tcagccttaa tttccatgcc ttccagttga ccggatttca ggcatttagc
    10441 atcgtggctc cattgcagat ttttcgccac gtcttctttt aaaccaaact gcgctaccag
    10501 ttccggtgtc agacaagcat agcttttgct cgcgtcatct tcaccagcat accagtaaat
    10561 atcgtactct tccgccaatg gctgtttatt tagttgaacc tgtaaattgt attttccggg
    10621 ctcaacatat ccctggctgg aaaaacgctt cagatcaatt tttgtgtcgc cttttaattc
    10681 cagaaaacgg gaatcaaact gaatgtcgtc ttcagcccag acactgctat aactccctga
    10741 cattgccagc gcaatgcacc acggaagaat ccgtaatcga aaaagagaat ggtcaggcat
    10801 acacgtttct atccgttttt attcttgttc ggtcaaaatc catttaaatc ccccatagta
    10861 gtgtcgctat gggggtatgc cttacaggta agtaatctgg aaggtggtat ttgcttcgaa
    10921 attacccaga tctggcgcat cagctgcgcc gaccagccag gctttaaagt tcagtgtctg
    10981 cttcgctttg cctttattgg tagaagtgtc tttcactatt ttctggtcaa tacccatgcc
    11041 gcttttgtaa gaggtaccca gagagtcacc gatcgccagg ctaacattgt taaatgccgc
    11101 accggtatcg gtattgaaaa tggtgtaata attgccgcta tttgcagaag aaacggtacc
    11161 agtaaaggta gtagtcattg tttcctgagt atcaaatacg caatcttgca gacgaatctg
    11221 gaaacttttc ggcgtggtgg tgccgttatt tttcagtttt tcggcaccga tatcacccat
    11281 atcaacttcc agtttgtgat cgtccggaac gatagaacac actgagtctt caatggtgcc
    11341 gtaaaaacgt gcagtaccgt tgttacccgc agcgattgtt gccgtagagg tgaacacgcc
    11401 catcaccatt gccgcagcaa tagctgtttt aacaaactta ctcattattt gtccttaaac
    11461 tataaataat taaaaatata aacattgcaa tacattgatt cagtcaatag ccaatgttta
    11521 tacccagcca ttgaatacgg catgcgatcc aaaaagaaca ggtcgtagaa aattgtattc
    11581 gttggtgtgg ggccgcatag tgatgtttcc acacccctga tgcaatttaa ttttactaaa
    11641 gagtaaatgt agatttaatt aatatattga cagaagaaag gttatcttaa ggacatcctt
    11701 aaaaaatacg gaataagtat ttagaaaaaa tagaaagtat atcaataatg aatatcaaca
    11761 gcttaaaaaa caaacaatga acatataatg cgtttggcat ttatccactt ttgacaatat
    11821 aatatataaa cactatagcc agaatttaat caagtttcaa ttacatcctg cctgattatt
    11881 caactctatt ttctctatta tctgtattaa taaatgacaa tgagtgtcaa acagacatta
    11941 ttcatttcaa catgtaactt tgggcaaaat caagatacgc gcagaagaat aatttccgaa
    12001 tgaaagtaga aagtgtttta tttatagatg gggagagtct tcgatagtag tgaggttgac
    12061 agatatccgc tgcctgatga gcaattatgc ccaatgaatc tacctcattg ggcataattt
    12121 ggaactcatc agatagcttt tgccatcttc agattacacg ggctcatctg ccagttgaat
    12181 gtgccgtttc cgctttcatc aagcgttacg ctggcaatgg ccgaggtggt aaacataggc
    12241 ggcgtttcgc ccggacataa ctcggcaacc agatacccga ctaacggtaa gtgagagatc
    12301 accagtaccg aagcaacacc ttcattggtc aacgcctgca aataggcact gaccagacca
    12361 acatcgccgc agggcgttaa ctccggcaga acttctgcac tggaaggcag gttcagacaa
    12421 tcccctactt cttccagtgt ttgctcggct cgcaggaacg ggctcaccag aacgcgttcg
    12481 atttccactt tttgaccttt cagccagttc gccatcaggc gagattcgtc acaaccatta
    12541 gtggtcagag gacgaaccga atcactggcg gcatcgaggg ctgcgtcgcc gtgacgcatg
    12601 ataaaaactt gcatattgca ccgcttttgt taaccagatt cgcccatttt ctttcatctg
    12661 aaaccagaga aagaaaacgg tggccggcat tgtgccttat ccattcaccg aatgaaacgc
    12721 tgttttttac ctcaatggcg taagtatagt caatatgcgt ttacattttg accaacactc
    12781 cgccattcag cgcggattca tatagctttg accttcttat tgcaggtcag ttgcagttgt
    12841 tttccaaaaa ctttccccac gcgcgcccat ctcgaccaaa cgctcgcaag gggtaaaacg
    12901 ggaaccatac tgcgtggcca gtcgttgcat tattgcaacc acttcgcccg cgccgagaga
    12961 atcgatatag cggaacggtc caccgagaaa tggcggaaaa ccaatgccaa ataccgcacc
    13021 aatatccccg tcacgcacgc tacggataac ctgctcatca acacaacgta ctgcttcatt
    13081 cagcatcaac atcacacacc gttcagcaac ctgcggtgcg gagagtcgcc cctgcccttg
    13141 tgcgccaatg agcgggtaaa tggcaggatc gacctgtttt ttgcttttac gccctttctg
    13201 accataaaga tagaaacccc gaccattttt tctgcctttg cgatcgtcgt tcaaaattga
    13261 agaaacaaca tttgcaggcg cgctaaaacg ttctccataa gcggcttcca gtacaggaat
    13321 aattttagtc ccggtgtcga ttcctacctc atccaaaagt tggattgggc ctaccggaaa
    13381 accaaatttc actagcgcag catcaatgtg ctctacccgt tcaccttcgg tcagcatgcg
    13441 gatagcttca ttaatgtaag gcgctaagat gcgattgacg taaaacccgg ctttgtcacg
    13501 cacgacaatg ggcgttttgc cctgtttttt cgccagtttt accgtagtgg cgatggtttg
    13561 cgctgatgta cccgcatgag gaataatctc caccagcggc attttttcca ccggactgaa
    13621 gaaatgcagg ccgataactt gctcaggtcg cgtggcgtga gcggcgatat caccaatcgg
    13681 caaagatgac gtattcgaag caaagatggt atgagcggcg caattttgct ccacttccgc
    13741 caccatctgt tgttttaatt cgagattttc aaacaccgct tcaataatca gatcgcgatg
    13801 ggcaaagccg cgatagtccg tcgttccgga gattaatgcc agctgtttgt cacgttcgct
    13861 ggctttgaga tgacgacggc gaactttgcc ctccagctga tcccaactgt acttcagcgc
    13921 atgatttatg ccctgcgggt tgatatcttt aattctgacc ggaagccccg ctttacaagc
    13981 agtgacataa gcaataccgc cgcccatcaa gccaccacct aaaattccca cgctgtttaa
    14041 tggcgcaggc ggtgcatcac taccgggatc tttcttcacg tccgtactgg caaaaaagat
    14101 actacgcaaa gcttgcgatt gtggcgtcat cgccagttcg ccaaacgccc gagcttcggc
    14161 gtcataaccg ctgctggtgc cctgcgctaa tcccgtttca acaacctcca ggatgcgttc
    14221 tatcgccgga taattgcctt gagttttgtg ttctgttttc ttgccgacca ttttgaacag
    14281 cagcgcacga cctaacggcc ccgccagaat acgctcgcgt acaggtagag ggcgggaaga
    14341 tgggcgatcc ttcttcgcca gctcaacagc ggcttccagc aaaatggagt gcggaacaac
    14401 gtcatccacc agccccagct ttaatgcctg tttcgcccga agttgttttc cggtgagaat
    14461 catctctaat gctgtactga cgcctatcag acgcggtaaa cgctgggtgc cgcctgaacc
    14521 gggtaacaat ccaagttgta cttcaggcaa accgagcacc gttttaggat cgtcagtaca
    14581 aacgcgaccg tggcacgcca gcgccagctc cagcccaccg cccaggcaag cgccatgaat
    14641 agccgcgata accggaatgg gcaaagcatg aatctccgcc atcaactgtt ggccctgccg
    14701 cgccagtgct tccgcttctt gcgccgtttt gcagttgccg atcatgttga tgtctgcgcc
    14761 agcaatgaag ttgtccggtt tagcggagac aaacaccacg cctcgcaact ctttgttttc
    14821 acggagttgc ttaataatgg cgcgcacctg cgaggcaaac tccgccttca gggtattcat
    14881 tttctcaccc ggtacgtcga tggtgataac ggcaatgttg tccagacgaa cattaagggt
    14941 aaacgctgat gtcatttcca ttattccgcc tccagaacca ttgccgcgcc aagcccaccg
    15001 gcagcacagg cggtaactaa accaaatcca ccgccgcgac ggcgaagttc atgcaatgtc
    15061 tgggtaatca tccgcgcgcc ggtcgccgcg aagggatgtc cgtaagcaat cgaaccgcca
    15121 agcacgttaa atttgctatc gtccacttcg ccagtggcat gtgcacgccc cagtacttca
    15181 cgagcaaaac gttcactacc cagcaactga atattcgcca gtgtctgagc tgcaaaggct
    15241 tcgtgcatat cgatcaatgt cagttcgctc atcgtcaaac cggcacgttc cagcgccagc
    15301 ggtgttgacc aggctggacc gagcaacatg tcctgccaga catcaatcgc agtaaatgcg
    15361 tagctgcgca gataccccag cggcaccagc cctaattctt tcacccggga ttcagtcatc
    15421 aggatcaccg ctgccgcgcc atcggtcagc ggcgtactgt ttgccgccgt taccgttccg
    15481 tgtttgcgat caaacgccgg gcgcagcttt gcgtaatcgg caagcgagga attaccgcga
    15541 atattgttgt cttcgacaag cggttgttta taaggaggga taaaggcagt catcacctct
    15601 tctttgagtt ttccgtctga ccatgcctga gcggcacgct gatgcgaacg gtgcgctaat
    15661 gcatcttgct gttctcgggt gatgccgtag gttttcgcca tttgttcggc ggtgtcgccc
    15721 atccgcaagc cagtagaata ttctgctacc gcaggcggta cgggcattaa gtcgcgcaaa
    15781 cgcaggcgag agaagagttt caggcgctgg ctcatggttc gagctttgtt gacatcaacc
    15841 agcacgcgcg ccagtttttt actgacgcca attggcaata cggaagagga atctgcccca
    15901 ccggcaatcc ccgctcggat agttcccgcc atcaggcttt ctgcgacgtt tgcaaccgcc
    15961 tggaaactgg tagcgcaagc gcggctgacg ctgtaagcat cggtatgcac attcattccg
    16021 gtaccgagaa caatttcacg cgcaatgttg ggggcttcag gcatttgtac gacctgacca
    16081 aagaccagct gttcaatcac ttcagcgggg atctcgctgc gtgccagcag ttcgcctacc
    16141 acaatcttcc ctaaatcaac cgcgggaatg ccatgaaaag ccgtcgcctg acgggcaaaa
    16201 ggcgtacgta aaccgctaac aatggcgata cgatcgccct ggcgggtaac cagcggtaaa
    16261 acctgaccca taacactccc ctgtaaaaaa ataaataaag tggtctgacc tgatcatagt
    16321 cttaaccatt tttttacatt tagccaagtg gagaaaaggg aaagtgggag ctatgacaca
    16381 gagagaaaag aagaagagaa aagaaaacgc ccctgccagc aactgacagg ggcgtacgta
    16441 cccgagagga attaacgcag acccagctgg aagatcagca tttcagcttc gcaggcaaaa
    16501 gtgaaatcga tatccaggcg cacaccgtca gactcttcag tgaaagtcgg ggtgattttg
    16561 cacggttcgg attccacgct acgggctttt tcagtcagcg ccgccagcgt ttgttctgct
    16621 tctgcgcggc ttgcaaacac gcggctgtaa gacgcggtgc agtcggagtt gtccataatg
    16681 gtgccaacat ccatacagca gcaaaccggg gtttcatcag cactacattt actcatcgtt
    16741 gatttcctct gtatgtgcac ccaaggtgcc agataaacgt tgtggatatt ttacgcttcc
    16801 ggaaagtgct gctccagttg ttaattctgc aaaatcggat aagtgaccga aatcacactt
    16861 aaaaatgatc taaaacaaaa ttcacccgaa tccgcgagtg cgcaacctcc aaattttgcc
    16921 agctggatcg cgtttcttag atcatatttg aaaaaagata gaaacatact tgcaacattc
    16981 cagctggtcc gacctatact ctcgccactg gtctgatttc taagatgtac ctcagaccct
    17041 acacttcgcg ctcctgttac agcacgtaac atagtttgta taaaaataaa tcattgaggt
    17101 tatggtcatg agccagaaaa ccctgtttac aaagtctgct ctcgcagtcg cagtggcact
    17161 tatctccacc caggcctggt cggcaggctt tcagttaaac gaattttctt cctctggcct
    17221 gggccgggct tattcagggg aaggcgcaat tgccgatgat gcaggtaacg ttagccgtaa
    17281 ccccgcattg attaccatgt ttgaccgccc gacattttct gcgggtgcgg tttatattga
    17341 cccggatgtg aatatcagcg gaacgtctcc gtctggtcgt agcctgaaag ccgataacat
    17401 cgcgcctacg gcatgggttc cgaacatgca ctttgttgca ccgattaacg accaatttgg
    17461 ttggggcgct tctattacct ctaactatgg tctggcgaca gagtttaatg atacttatgc
    17521 aggcggctct gtcgggggta caactgacct tgaaaccatg aacctgaact taagcggtgc
    17581 gtatcgctta aataatgcat ggagctttgg tcttggtttc aacgccgtct acgctcgcgc
    17641 gaaaattgaa cgtttcgcag gcgatctggg gcagttggtt gctggtcaga ttatgcaatc
    17701 tcctgccggg caaactcctc aagggcaagc attggcagct accgccaacg gtatcgacag
    17761 taataccaaa atcgctcatc tgaacggtaa ccagtggggc tttggctgga acgccggaat
    17821 cctgtatgaa ctggataaaa ataaccgcta tgcactgacc taccgttctg aagtgaaaat
    17881 tgacttcaaa ggtaactaca gcagcgatct taatcgtgcg tttaataact acggtttgcc
    17941 aattcctacc gcgacaggtg gcgcaacgca atcgggttat ctgacgctga acctgcctga
    18001 aatgtgggaa gtgtcaggtt ataaccgtgt tgatccacag tgggcgattc actatagcct
    18061 ggcttacacc agctggagtc agttccagca gctgaaagcg acctcaacca gtggcgacac
    18121 gctgttccag aaacatgaag gctttaaaga tgcttaccgc atcgcgttgg gtaccactta
    18181 ttactacgat gataactgga ccttccgtac cggtatcgcc tttgatgaca gcccagttcc
    18241 ggctcagaat cgttctatct ccattccgga ccaggaccgt ttctggctga gtgcaggtac
    18301 gacttacgca tttaataaag atgcttcagt cgacgttggt gtttcttata tgcacggtca
    18361 gagcgtgaaa attaacgaag gcccatacca gttcgagtct gaaggtaaag cctggctgtt
    18421 cggtactaac tttaactacg cgttctgata acacgttcgc ctggataaag tcacctgcat
    18481 agcaggtgac tttaactccc ccacttcacc gaaagtagtg tcctcattgc ttacctcctg
    18541 agttttgcaa acagcctgat ggcagcttgc cctttcaaat caataagcgg tatccacaga
    18601 caaacctgaa gggaaaggca tattttcagg cgttctgctc gtccttctca aagagtttac
    18661 ttttctgaat ttccaggata ctcacccccc ccctggctat tgtgcgctca tacactcaaa
    18721 ttaaagatag gttctaaata aatgagcgtt ttttgatagt ctatttcatt aggtaatata
    18781 tatttgtaac aaatcaatca aaatggaata aaatcatgct accatctatt tcaatcaaca
    18841 ataccagcgc aacttaccca gaatccatta atgaaaataa caatgatgaa gttaatggat
    18901 tagtacaaga gctcaaaaac ctttttaatg gtaaggaagg aataagcacc tgtattaaac
    18961 atctacttga gcttataaaa aacgccatac gagtaaacga cgatccttat agatttaata
    19021 ttaataattc ctcagttact tatattgata ttggctccaa tgatacagac catattacta
    19081 ttggtatcga caaccaagaa ccaatagaat tacctgcgaa ctataaagac aaagaactcg
    19141 tccgtactat cattaatgac aacatagttg agaagactca tgatatcaat aacaaggaaa
    19201 tgatcttcag cgcattaaaa gaaatatatg atggagatcc tggttttatc ttcgataaga
    19261 tatcacacaa actcagacat acggtaacgg aatttgatga gagcgggaat agcgaaccaa
    19321 cggacttatt tacctggtac ggtaaagata aaaaaggcga ctctctcgct attgtaatta
    19381 aaaataaaaa cggaaatgat tacttatctc tcggttacta cgatcaggac gactaccaca
    19441 ttcaaagagg aattcgtatt aatggtgata gtctcaccca atattgtagt gaaaaagcca
    19501 ggaatgcttc agcgtggttt gaaagcagta aagctatcat ggcagaatca tttgcaacag
    19561 gttccgatca tcaggttata aacgagctca acggggaaag actgagagaa cctaacgaag
    19621 tttttaaacg tttaggtcga gcaataagat ataattttca agtggacgat gcaaaattta
    19681 gacgcgacaa tgtaaaagaa ataatttcta atttattcgc taacaaagtt gacgttgacc
    19741 atcctgaaaa taaacacaaa gactttaagg atctcgaaga taacgttgaa aaaagacttc
    19801 aaaatcgcca ggcgaaatat caaaatgaaa ttaatcaact atctgcgcca ggtgttaatt
    19861 ttgatgatat ttaaaattat ttttccagtt ttttgatttt tcaacgcctg aatatcggaa
    19921 atagtcagtg acgaacatca gtatttttac atcattacgt ccgggtcatt gtcggtttat
    19981 ctccttttac ttgtggtaat tgagggataa caacaaaaaa ggtgagtttt gcgactcacc
    20041 ttttttattt gtttcttatt cagaatcaat atcttttaaa tcatcctgaa tcgcttgtgc
    20101 gttcggattt tcctgcggtt tgagttcgcc gccattagcg atgaaatcat gacgctggaa
    20161 gtacgcttcg cgcaccataa tataaggatc ggacgactga cgcagcagac catcggaatc
    20221 caacaactgc gcacgagttt ctatcccttc aagcgtccat ttacccacag acatcggcca
    20281 ggtcagccag gaaagaaccg ggtaaagacc atccgccata tcaccaccgt catcacgcag
    20341 cgtgaagcta ccgtagaacg gtaactggac gtaaggccca taacccacgc cataatgacc
    20401 aagcgtacta ccgaagcggt gaggttcagt ccgttgcagt ttcgggttcg ccatccctgc
    20461 aacatcaata aaaccgccca tccccaaaat ggtgttcagg aaaaagcggg taaagtggac
    20521 catcccctga taagggtcgc cctgcaagaa gtagttaacc atcaccgcag gttcttcaag
    20581 gttgccagta aagttgctca aaccattacg cgccggttgc ggaacataat cacgccaggc
    20641 gacagcgacc ggtcgaacaa tatacgggtc taatacgttg aagttgaagt tgtacatggt
    20701 gcggttgaac ccttctaacg ggtcagaacg cccttgctga tctgtaccgg aactcgcaca
    20761 ccccaccaga agcgtagttc ccagagcaag cgccgacagg cgaagcttca taaatgtctc
    20821 cctgtttttt ttatggctta tgcagtttgc catccatgac ggaacgatac cgtatccgcc
    20881 tgtttaggtg tgggcgattg taacagcacg tcaactgatg tccagacgcc ctgatttgct
    20941 gatttgatca tagcctggta atcgccgccc tgtaggctac ttgattctat agaaacagaa
    21001 aaaggcaaac gcagcctttt ctacattttc agagtaactc ccgccagttg cgagcaaaaa
    21061 agccgctacg ctttagctat acgtgctaat tcaagagaag agaccatgga caacgacaaa
    21121 attgatcaac acagcgacga aattgaagtt gagagcgaag aaaaagagcg cggcaaaaaa
    21181 atagaaatag atgaagaccg actcccctcc cgggcgatgg caattcatga gcatatccgc
    21241 caggatggtg aaaaagagct ggaacgcgac gcaatggcgc tactgtggtc agccattgcg
    21301 gcgggtctgt cgatgggcgc ttcgttactg gcaaaaggga tatttcatgt cgaactggaa
    21361 ggtgtgccgg gcagcttctt actggagaat ctcggttata cctttggttt tattatcgtc
    21421 attatggccc gccagcaatt atttaccgaa aacaccgtga ctgcggtact acccgtcatg
    21481 caaaaaccga caatgagcaa cgtcggctta cttatgcgat tatggggcgt cgtgctgctg
    21541 ggtaatattc tcgggacagg tattgctgca tgggcatttg aatatatgcc tatcttcaat
    21601 gaagaaactc gcgatgcatt tgtcaaaatc ggcatggatg tgatgaagaa cacccccagc
    21661 gagatgtttg ccaacgcgat catttccggc tggctgatcg ccactatggt atggatgttt
    21721 cctgcagcag gtgcggcaaa gattgtggtg attatattga tgacctggct tattgccctg
    21781 ggtgacacca cccatatcgt ggtcggttct gttgaaatcc tctatctggt gtttaacggt
    21841 acgctgcact ggagcgattt catctggccc ttcgcactac ctactttagc ggggaacatc
    21901 tgcggcggca cctttatctt cgcgttaatg agtcatgcac agattcgtaa cgacatgagc
    21961 aacaagcgta aagcagaagc acgccaaaaa gcagaacgtg cggaaaacat taagaaaaat
    22021 gataaaaacc cggcataaat ggcgagggtt taagcaatcg agcggcagcg tacttacccc
    22081 gcactccatt agcgggtata ctcatgccgc attgtcctct tagttaaatg gatataacga
    22141 gcccctccta agggctaatt gcaggttcga ttcctgcagg ggacaccaga tacccttcaa
    22201 acgaaatcta ccttcacccc gtaaaagatg ggtttggcag cacacttgcc ttatatctac
    22261 tcatttttac tgcaacaggt tgaaatctca gcactgtcag aaagcgctga tgactaaaca
    22321 gccctgggcc gggcgatgta accatcacac agaatcctga tagcgaaata tggcgtgact
    22381 cgatacttca ctccgcaatg cattccttga tgaattcgca ggaccgtgat acacgggaca
    22441 ggtcactgaa tgacgacaat gtcctggaaa tcagcgaacc gcgcatctga agtacatttg
    22501 agcgactgta ccagaacatg aatgaggcgt ttggattagg cgattattag cagggctaag
    22561 cattttacta ttattatttt ccggttgagg gatatagagc tatcgacaac aaccggaaaa
    22621 agtttacgtc tatattgctg aaggtacagg cgtttccata actatttgct cgcgtttttt
    22681 actcaagaag aaaatgccaa atagcaacat caggcagaca atacccgaaa ttgcgaagaa
    22741 aactgtctgg tagcctgcgt ggtcaaagag tatcccagtc ggcgttgaaa gcagcacaat
    22801 cccaagcgaa ctggcaattt gaaaaccaat cagaaagatc gtcgacgaca ggcgcttatc
    22861 aaaatttgcc acgctgtatt tgaagacgga tatgacacaa agtggaacct caatagcatg
    22921 taacagcttc actaatgaaa taatccaggg gttaacgaac agcgcgcagg aaaggatacg
    22981 caacgccata atcacaacac cgataagtaa tgcatttttt ggccctaccc gattcacaaa
    23041 gaaaggaata atcgccatgc atagcgcttc gagtaccacc tggaatgagt tgagataacc
    23101 atacaggcgc gttcctacat cgtgtgattc gaataaacct gcataaaaga caggaaaaag
    23161 ttgttgatca aaaatgttat agaaagacca cgtccccaca ataaatatga cgaaaaccca
    23221 gaagtttcga tccttgaaaa ctgcgataaa atcctctttt tttacccctc ccgcatccgc
    23281 cgctacgcac tggtgatcct tatctttaaa acacatgttg atcatcataa atacagcgcc
    23341 aaatagcgag accaaccaga agttgatatg gggactgata ctaaaaaata tgccggcaaa
    23401 gaacgcgcca atagcatagc caaaagatcc ccaggcgcgc gctgttccat attcgaaatg
    23461 aaaatttcgc gccatttttt cggtgaagct gtcaagcaaa ccgcatcccg ccagataccc
    23521 caggccaaaa aagagcgccc ccagaattag acctacagaa aaattgcttt gcagtaacgg
    23581 ttaataaacg taaatcataa acggtccggt caagaccagg atgaaactca tacaccagat
    23641 gagcggtttc ttcagaccga gtttatcctg aacgatgccg tagaacatca taaatagaat
    23701 gctggtaaac tggttgaccg aataaagtgt acctaattcc gtccctgtca accctagatg
    23761 tcctttcagc caaatagcgt ataacgacca ccacagcgac caggaaataa aaaagagaaa
    23821 tgagtaactg gatgcaaaac gatagtacgc atttctgaat ggaatattca gtgccataat
    23881 tacctacctg tcgttaaaaa attcacgtcc tatttagaga taagagcgac ttcgccgttt
    23941 acttctcact attccagttc ttgtcgacat ggcagcgctg tcattgcccc tttcgccgtt
    24001 actgcaagcg ctccgcaacg ttgagcgaga tcgataattc gtcgcatttc tctctcatct
    24061 gtagataatc ccgtagagga cagacctgtg agtaacccgg caacgaacgc atctcccgcc
    24121 cccgtgctat cgacacaatt cacagacatt ccagcaaaat ggtgaacttg tcctcgataa
    24181 cagaccacca ccccttctgc acctttagtc accaacagca tggcgatctc atactctttt
    24241 gccagggcgc atatatcctg atcgttctgt gtttttccac tgataagtcg ccattcttct
    24301 tccgagagct tgacgacatc cgccagttgt agcgcctgcc gcaaacacaa gcggagcaaa
    24361 tgctcgtctt gccatagatc ttcacgaata ttaggatcga agctgacaaa acctccggca
    24421 tgccggatcg ccgtcatcgc agtaaatgcg ctggtacgcg aaggctcggc agacaacgca
    24481 attgaacaga gatgtaacca ttcgccatgt cgccagcagg gcaagtctgt cgtctctaaa
    24541 aaaagatcgg cactggggcg gaccataaac gtaaatgaac gttccccttg atcgttcaga
    24601 tcgacaagca ccgtggatgt ccggtgccat tcatcttgct tcagatacgt gatatcgact
    24661 ccctcagtta gcagcattct ttgcattaac gccccaaaag gatcatcacc gacccgacct
    24721 ataaatccac ttattccgcc taatctggcg attcccaccg caacgttagc tggcgcgccg
    24781 ccaggacaag gcagtaggcg cccgtctgat tctggcaaga gatctacgac cgcatcccct
    24841 aaaacccata ctttggctga catttttttc ccttaaattc atctgagtta cgcatagtga
    24901 taaacctctt tctcgcaaaa tcgtcatgga tttactaaaa catgcatatt cgatcacaaa
    24961 acgtcatagt taacgttaac atttgtgata ttcatcgcat ttatgaaagt aagggactat
    25021 atttttataa aagttaacgt taacaattca ccaaatttgc ttaaccagga tgattaaaat
    25081 gacgcaatct cgattgcatg cggcgcaaaa cgcactagca aaacttcatg agcaccgggg
    25141 taacactttc tatccccatt ttcacctcgc gccacctgcc ggatggatga acgatccaaa
    25201 cggcctgatc tggtttaacg atcgttatca cgcgttttat caacatcacc cgatgagcga
    25261 acactgggga ccaatgcact ggggacatgc caccagcgac gatatgatcc actggcagca
    25321 tgagcctatt gcgctagcgc caggagacga taatgacaaa gacgggtgtt tttcaggtag
    25381 tgctgtcgat gacaatggtg tcctctcact tatctacacc ggacacgtct ggctcgatgg
    25441 tgcaggtaat gacgatgcaa ttcgcgaagt acaatgtctg gctaccagtc gggatggtat
    25501 tcatttcgag aaacagggtg tgatcctcac tccaccagaa ggaatcatgc acttccgcga
    25561 tcctaaagtg tggcgtgaag ccgacacatg gtggatggta gtcggggcga aagatccagg
    25621 caacacgggg cagatcctgc tttatcgcgg cagttcgttg cgtgaatgga ccttcgatcg
    25681 cgtactggcc cacgctgatg cgggtgaaag ctatatgtgg gaatgtccgg actttttcag
    25741 ccttggcgat cagcattatc tgatgttttc cccgcaggga atgaatgccg agggatacag
    25801 ttaccgaaat cgctttcaaa gtggcgtaat acccggaatg tggtcgccag gacgactttt
    25861 tgcacaatcc gggcatttta ctgaacttga taacgggcat gacttttatg caccacaaag
    25921 ctttttagcg aaggatggtc ggcgtattgt tatcggctgg atggatatgt gggaatcgcc
    25981 aatgccctca aaacgtgaag gatgggcagg ctgcatgacg ctggcgcgcg agctatcaga
    26041 gagcaatggc aaacttctac aacgcccggt acacgaagct gagtcgttac gccagcagca
    26101 tcaatctgtc tctccccgca caatcagcaa taaatatgtt ttgcaggaaa acgcgcaagc
    26161 agttgagatt cagttgcagt gggcgctgaa gaacagtgat gccgaacatt acggattaca
    26221 gctcggcact ggaatgcggc tgtatattga taaccaatct gagcgacttg ttttgtggcg
    26281 gtattaccca cacgagaatt tagacggcta ccgtagtatt cccctcccgc agcgtgacac
    26341 gctcgcccta aggatattta tcgatacatc atccgtggaa gtatttatta acgacgggga
    26401 agcggtgatg agtagtcgaa tctatccgca gccagaagaa cgggaactgt cgctttatgc
    26461 ctcccacgga gtggctgtgc tgcaacatgg agcactctgg ctactgggtt aacataatat
    26521 caggtggaac aacggatcaa cagcgggcaa gggatccgcg tcactcttcc cccttcacga
    26581 ccttcaataa tatgcaatgc agcttcccgc ccgataatgt catgtggaag ctgaattgtg
    26641 gtcagcggcg gtaaaaacag atgcccgacg ccaaccagat tatcaaagcc cattacggcg
    26701 acatcctgcg ggattcgtac ccccttcgcc agaagaacct gataagccac aaaggctgcg
    26761 cgatcgttac cacatatcag aacatcaaaa tctggtttgc ccgatttgaa gtggtcattg
    26821 agtaaacttg cgagatcggt gtagtgatca tcacctgttg ccatgtgaaa ttgtttcacc
    26881 tcagccagat ctcgtccagc atcacgccag gcctgctcaa atccctgccg acgataccct
    26941 gttgccaacg cactttccgg tagccagaag cataacggtt gacgatagcc cgccgcgagc
    27001 aaatgctgtg ttgattcata ttgtgcagtg taatcatcag ggatataact gggtaacgct
    27061 gggtcatccg ccacacagtt cgccaataca atattttcac catacagaga ctcaggcagc
    27121 gtgatatgtc gcagccccat tgtagtatag ataatgccat ccggacggtg ggcaagcagc
    27181 tgacgtgccg cgcgggcagc gtcatcttca gaaaaaatat tgattaaaaa actattccag
    27241 ccgaactcgc tggcggtttg ctcaatggca agcagaatat caacagagaa aggagtggta
    27301 gccgtgtcct gcgccagcac ggcgagagtc gacggcttac gtccttgagc gcgcatctta
    27361 cgggcggaaa gatcaggaac ataattcagg gtctggattg cctgcaatac gcggtcacgc
    27421 gttgcaggac gcacagattc tgcattatgc atcacccggg agactgtcat catcgacact
    27481 cccgccaggc gtgcgacatc ctttaatgaa gccataccca agccgtttgc cgtaaaacgg
    27541 gcactgtagc agaaacagac gtcactggcg agatccaacg atattctcaa cgccctatca
    27601 cccgacacgg caatacaata aaaaataaca ataattgccg gataattgta ctcaattccg
    27661 cctctgttct cgccaacgag taaccatata ggcaagtaag taaaacatgc caactggcat
    27721 cgccaacctg gagaagaaaa tataaatatg gatcattgtg ctgccgctgt atcccgacat
    27781 cagcccggaa attattgcga ttgctatcgg ttcaggtgca attggctgca cgatcgttac
    27841 ggactcgctt ttctggctag tgaagcaata ttgcggcgct acgctcaatg aaacatttaa
    27901 atactatacg acagcgacat ttatcgcttc agtcgtcgct ctggcgggca cattcctgct
    27961 gtcatttatc atctaagcgc aaagagacgt actatggaaa acgctaaaat gaactcgctc
    28021 atcgcccagt atccgttggt aaaggatctg gttgctctta aagaaaccac ctggtttaat
    28081 cctggcacga cctcattggc tgaaggttta ccttatgttg gcctgaccga acaggatgtt
    28141 caggacgccc atgcgcgctt atcccgtttt gcaccctatc tggcaaaagc atttcctgaa
    28201 actgctgcca ctggggggat tattgaatca gaactggttg ccattccagc tatgcaaaaa
    28261 cggctggaaa aagaatatca gcaaccgatc agcgggcaac tgttactgaa aaaagatagc
    28321 catttgccca tttccggctc cataaaagca cgcggcggga tttatgaagt cctggcacac
    28381 gcagaaaaac tggctctgga agcggggttg ctgacgcttg atgatgacta cagcaaactg
    28441 ctttctccgg agtttaaaca gttctttagc caatacagca ttgctgtggg ctcaaccgga
    28501 aatctggggt tatcaatcgg cattatgagc gcccgcattg gctttaaggt gacagttcat
    28561 atgtctgctg atgcccgggc atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc
    28621 gtggaatatg agcaagatta tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct
    28681 gacccgaact gtttctttat tgatgacgaa aattcccgca cgttgttcct tgggtattcc
    28741 gtcgctggcc agcgtcttaa agcgcaattt gcccagcaag gccgtatcgt cgatgctgat
    28801 aaccctctgt ttgtctatct gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc
    28861 gggcttaaac tggcgtttgg cgatcatgtt cactgctttt ttgccgaacc aacgcactcc
    28921 ccttgtatgt tgttaggcgt ccatacagga ttacacgatc agatttctgt tcaggatatt
    28981 ggtatcgaca accttaccgc agcggatggc cttgcagttg gtcgcgcatc aggctttgtc
    29041 gggcgggcaa tggagcgtct gctggatggc ttctataccc ttagcgatca aaccatgtat
    29101 gacatgcttg gctggctggc gcaggaagaa ggtattcgtc ttgaaccttc ggcactggcg
    29161 ggtatggccg gacctcagcg cgtgtgtgca tcagtaagtt accaacagat gcacggtttc
    29221 agcgcagaac aactgcgtaa taccactcat ctggtgtggg cgacgggagg tggaatggtg
    29281 ccggaagaag agatgaatca atatctggca aaaggccgtt aataacgttt caacgcagca
    29341 tcgcaatcct ttccctgggt gagcgatgct gccgatggcg cagacttaag atccccggtc
    29401 ttacccgcta taaccccctc cttttatcat cacccaatgc ctttcgctgt aaacggcggt
    29461 ttcgcaaacc aaaccaaaac cgtaagtaag ataaaagcaa tagccgccat acggaaaatc
    29521 tcatttgcag aaatagaaag tgactgctgg gttatttcat tattaatttc attaagaact
    29581 cctgaaagcg aaccatagta tttatccata atttgtgacg atgaattaaa cacggggtta
    29641 aattgatcga tggttgctgt caactgacta tggtgtaacg attcgcgtcg tccccacagc
    29701 gtcattgtca acgacgtacc aactgatcct gacaaggtac gaaaaaaatt actcatactc
    29761 gaggcattgg caaatttatt atctggcaag cctgaaaacg aaatcgttgt taagggtaaa
    29821 aagaaacagg caacggcgaa tccctgaaaa aactgcggca aaatgatgcc tgtaaaatca
    29881 atcgttggca taaatgtcac agaacgccag taatagcaaa ccgcatacat caaaaaacta
    29941 aatgtcacta acaaccgcat gtctattttg ttgccataac gtcctatcaa aggtgaaatt
    30001 aatagtggca tgatgccgat gggcgcataa gcaagtccgg cccatatcgc attatacccc
    30061 atcgtttcct ggagtaactg cggcataagg acgatcgctc cagagtaaaa taaatacgcg
    30121 catgtgatac tcacaatacc aatggtgaag ttacgggact taaacaaact gagatcaaga
    30181 atcgggttct ctgaggtcga ctcccaaatg actaaagaga tcagagaaat aactgatact
    30241 actgttaata ttattattgt actcgagttg aaccaatcca gatcgcgccc tttatcaagc
    30301 ataatttgca agccaccaac accgagcact aacagggtca gtcctggtag attcattttg
    30361 accggtgaag tctcagtttc tcttccttta agtaaggtta agcataatgt caggacgata
    30421 atccccatag ggacattgat taaaaatatc caaccccagc taaagttatc acaaatataa
    30481 ccgcccaata tcggcccaca tatcggagcg ataatcacgg tcattgacca taatgccaga
    30541 gcaaatgttc ttttttctgg cggataattc cttaataata aactctgtga cagtggaatt
    30601 aacggccccg ccattaaccc ctgaacgact ctaaaaaata tcagcacatc aagattggtc
    30661 gataggctac acattaatga agacagcgaa aaaaaggtta ctgaaagtaa aaataatctt
    30721 aattcgccta ttctttgtgc caacctgcca gtaacaggga tcgcaatggc atttgctaca
    30781 ccaaacgagg tgataaccca ggtgccttcg tctgttgatg ctcccagaaa gccagatatt
    30841 gtcggtattg cgacgttaga aatagtggaa tccaacattt gcataaatgt cgctaatgac
    30901 aatgcaatag tgacgcacca taacgtccca ccggttaatg gtgccggagt tgatttagtg
    30961 attgccatta aaattgtcca ttatgcgaaa taatgttgct aatttctttt tctatcggac
    31021 tggtatcgat aactaaagcc ttactggtat aagccggcat ggaggtcacg gttgaagcca
    31081 gctcaggcat ctcggcaatg tcttcgttct tcgtatcaat agttgctgtc atcgataaac
    31141 caatacgcaa gggatgttcc atgagttctt ttggatcaag agaaacttca accggtacac
    31201 gctgaacgat tttgatccag ttccctgtcg cattttgtgc aggtaataag gagaacgcat
    31261 tgccggttcc catattgatc cctgtcaccc gaccatgaaa cacaacattt tcaccataaa
    31321 gatcgctgat aatattgacc gattgaccaa tccgtacatc cgtgagttgt gtttctttaa
    31381 agttggcatt aacccacatt tgacgtgccg gtacgacagc cattaacgat tgtccggggc
    31441 tcactgtttc gccgacctga acacttcgct gggcaatata gccggtaacc ggactcttaa
    31501 tatccgtacg tttaagcgcc aaccaggctt ctttagttgc atccgccgct tcaacgactt
    31561 gtggctgacg gtttaatggt gtgttcatta ctaaagcttt attcgcttta taagcctgga
    31621 tagcggcatt caatgccgct ttgctactta ttaacgtatc tttggtatgc tccagcgttt
    31681 cttttgaaat aaccccctgc ttcgctaacg gcactcgacg gttataatct tctaaagatt
    31741 gttgatactg aatacgtgct gaagcgactt cggcactgta ttgtttatcc tgtaagtata
    31801 gtttattcgt ttgccgaaca atatttgcca gattattttt agctttattg agtgcgatag
    31861 tggcatcagt tttatccagt gaaactaaaa tgtcaccttg tcgaacgtag ttcgtatctt
    31921 tatgattaac gacagtgaca ctacctgaga cttgtgcaga aattggatct gcattccccg
    31981 tgacataggc gtcatctgta ctaatcatgt cttctaattc cattgaccaa taggcatagg
    32041 cacctgaaaa cgcaataaat aaaactaccg ccaataaaga aaagtatttt cttctgttag
    32101 aatgtttttt atttgaatta atctgttcca ctattatctc tcatttctca tagatgaaat
    32161 ttatgaattg aatgaaaaga gcattcattc ttcatatcaa acacagtgca tgtgttgcac
    32221 attatcccag gaacgttgga ggcctaattt aaatgaggtg caaaaaaaac aacagaggac
    32281 taaaccgtgg cttttgcaat acaattctta cgcctgtagg attagtaaga agacttatag
    32341 tgctaacttg aaactataaa tcaccggtac aatccctgat tttattgttg acatttcatt
    32401 tatgccgact atttatatgg tatacttgtc gaattatctt aaaggaagct cagattttct
    32461 tatttttatt gagaaaatga gatgacgcct tatgtctgta ttactacagg gagaagggag
    32521 atgcttcatt gcaaagggaa taatctatga acgcaataat tatt
//
LOCUS       DAMZET010000031        32546 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.031, whole genome shotgun sequence.
ACCESSION   DAMZET010000031 DAMZET010000000
VERSION     DAMZET010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.031"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1277)
                     /gene="fusA"
                     /locus_tag="POB34_001597"
     CDS             complement(<1..1277)
                     /gene="fusA"
                     /locus_tag="POB34_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDI5918570.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKA"
     gene            complement(1374..1844)
                     /gene="rpsG"
                     /locus_tag="POB34_001598"
     CDS             complement(1374..1844)
                     /gene="rpsG"
                     /locus_tag="POB34_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDI5918571.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(1941..2315)
                     /gene="rpsL"
                     /locus_tag="POB34_001599"
     CDS             complement(1941..2315)
                     /gene="rpsL"
                     /locus_tag="POB34_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDI5918572.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(2441..2728)
                     /gene="tusB"
                     /locus_tag="POB34_001600"
     CDS             complement(2441..2728)
                     /gene="tusB"
                     /locus_tag="POB34_001600"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HDI5918573.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(2736..3095)
                     /gene="tusC"
                     /locus_tag="POB34_001601"
     CDS             complement(2736..3095)
                     /gene="tusC"
                     /locus_tag="POB34_001601"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HDI5918574.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(3095..3481)
                     /gene="tusD"
                     /locus_tag="POB34_001602"
     CDS             complement(3095..3481)
                     /gene="tusD"
                     /locus_tag="POB34_001602"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HDI5918575.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(3481..4203)
                     /locus_tag="POB34_001603"
     CDS             complement(3481..4203)
                     /locus_tag="POB34_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI5918576.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(4370..5182)
                     /gene="fkpA"
                     /locus_tag="POB34_001604"
     CDS             complement(4370..5182)
                     /gene="fkpA"
                     /locus_tag="POB34_001604"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDI5918577.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            5403..5621
                     /locus_tag="POB34_001605"
     CDS             5403..5621
                     /locus_tag="POB34_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HDI5918578.1"
                     /translation="MQDLSLETRLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(5670..6260)
                     /gene="slyD"
                     /locus_tag="POB34_001606"
     CDS             complement(5670..6260)
                     /gene="slyD"
                     /locus_tag="POB34_001606"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDI5918579.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(6355..6555)
                     /locus_tag="POB34_001607"
     CDS             complement(6355..6555)
                     /locus_tag="POB34_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HDI5918580.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(6565..8370)
                     /gene="kefB"
                     /locus_tag="POB34_001608"
     CDS             complement(6565..8370)
                     /gene="kefB"
                     /locus_tag="POB34_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HDI5918581.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(8370..8921)
                     /gene="kefG"
                     /locus_tag="POB34_001609"
     CDS             complement(8370..8921)
                     /gene="kefG"
                     /locus_tag="POB34_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HDI5918582.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            9052..10965
                     /locus_tag="POB34_001610"
     CDS             9052..10965
                     /locus_tag="POB34_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5918583.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQADEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            10965..11987
                     /locus_tag="POB34_001611"
     CDS             10965..11987
                     /locus_tag="POB34_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDI5918584.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            11981..12199
                     /locus_tag="POB34_001612"
     CDS             11981..12199
                     /locus_tag="POB34_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HDI5918585.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            12253..13122
                     /locus_tag="POB34_001613"
     CDS             12253..13122
                     /locus_tag="POB34_001613"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HDI5918586.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(13177..13581)
                     /locus_tag="POB34_001614"
     CDS             complement(13177..13581)
                     /locus_tag="POB34_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HDI5918587.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            13883..14515
                     /gene="crp"
                     /locus_tag="POB34_001615"
     CDS             13883..14515
                     /gene="crp"
                     /locus_tag="POB34_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HDI5918588.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            14566..16656
                     /locus_tag="POB34_001616"
     CDS             14566..16656
                     /locus_tag="POB34_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDI5918589.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFAACSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(16723..17943)
                     /gene="argD/dapC"
                     /locus_tag="POB34_001617"
     CDS             complement(16723..17943)
                     /gene="argD/dapC"
                     /locus_tag="POB34_001617"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HDI5918590.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQFKGRARDFLYAGAGAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(18029..18592)
                     /gene="pabA"
                     /locus_tag="POB34_001618"
     CDS             complement(18029..18592)
                     /gene="pabA"
                     /locus_tag="POB34_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HDI5918591.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(18624..19226)
                     /locus_tag="POB34_001619"
     CDS             complement(18624..19226)
                     /locus_tag="POB34_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HDI5918592.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(19216..19383)
                     /locus_tag="POB34_001620"
     CDS             complement(19216..19383)
                     /locus_tag="POB34_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HDI5918593.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(19488..20060)
                     /gene="ppiA"
                     /locus_tag="POB34_001621"
                     /gene_synonym="rotA"
     CDS             complement(19488..20060)
                     /gene="ppiA"
                     /locus_tag="POB34_001621"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HDI5918594.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            20331..21512
                     /gene="tsgA"
                     /locus_tag="POB34_001622"
     CDS             20331..21512
                     /gene="tsgA"
                     /locus_tag="POB34_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HDI5918595.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            21774..24317
                     /gene="nirB"
                     /locus_tag="POB34_001623"
     CDS             21774..24317
                     /gene="nirB"
                     /locus_tag="POB34_001623"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HDI5918596.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            24314..24640
                     /gene="nirD"
                     /locus_tag="POB34_001624"
     CDS             24314..24640
                     /gene="nirD"
                     /locus_tag="POB34_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HDI5918597.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            24767..25573
                     /gene="nirC"
                     /locus_tag="POB34_001625"
     CDS             24767..25573
                     /gene="nirC"
                     /locus_tag="POB34_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HDI5918598.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            25592..26965
                     /gene="cobA"
                     /locus_tag="POB34_001626"
     CDS             25592..26965
                     /gene="cobA"
                     /locus_tag="POB34_001626"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDI5918599.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            27212..27379
                     /locus_tag="POB34_001627"
     CDS             27212..27379
                     /locus_tag="POB34_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HDI5918600.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            27623..29011
                     /gene="frlA"
                     /locus_tag="POB34_001628"
     CDS             27623..29011
                     /gene="frlA"
                     /locus_tag="POB34_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HDI5918601.1"
                     /translation="MTANSPLQRIGQEKGIAMGSQELQRKLGFWAVLAIAVGTTVGSG
                     IFVSVGEVAKAAGTPWLTVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGS
                     RPLAFLSGWASFWANDAPSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHL
                     RSVEGGAAFQTLITIAKIIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGI
                     SATSWSYTGMASICYMTGEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKL
                     ANSETPISDALTWIPALGSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFF
                     KCFGHVHPKYNTPDVSIILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWC
                     RKRDDYKPLWRTPAFGLMTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAF
                     WAKRSRQLNALS"
     gene            29032..30054
                     /gene="frlB"
                     /locus_tag="POB34_001629"
     CDS             29032..30054
                     /gene="frlB"
                     /locus_tag="POB34_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HDI5918602.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            30104..30934
                     /gene="frlC"
                     /locus_tag="POB34_001630"
     CDS             30104..30934
                     /gene="frlC"
                     /locus_tag="POB34_001630"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HDI5918603.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYDGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            30931..31716
                     /gene="frlD"
                     /locus_tag="POB34_001631"
     CDS             30931..31716
                     /gene="frlD"
                     /locus_tag="POB34_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HDI5918604.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            31816..>32546
                     /gene="phoB"
                     /locus_tag="POB34_001632"
     CDS             31816..>32546
                     /gene="phoB"
                     /locus_tag="POB34_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HDI5918605.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
BASE COUNT         7806 a         8728 c         8313 g         7699 t
ORIGIN      
        1 gctttggttt tcggttcaac tgcgatggag attaccggct cagggaattc catacgttcc
       61 agaatgatcg gcgcatccgg gtcacacagg gtgtcaccag tggttacgtc tttcagaccg
      121 atagcagcag cgatgtcgcc cgcgcgaact tctttgatct cttcacgttt gttagcgtgc
      181 atctgaacga tacgaccgaa acgctcacgt gcagctttca cggagttcag tacggtatca
      241 ccagagttaa ccacaccgga gtaaacacgg aagaaggtca ggttaccaac aaacgggtcg
      301 gtagcgattt tgaacgccag tgcagagaac ggctcgtcat cacttgcgtg acgttcagcc
      361 ggagtgtctt taccgtcgtc caggataccg ttgatcgcag gtacgtcaac cggggatggc
      421 aggtaatcaa ttaccgcatc cagcatcgcc tgaacacctt tgttcttgaa cgcagaacca
      481 caggttacca ggatgatttc gttgttcaga acgcgctgac gcagagcacc tttgatttct
      541 gcttcagtca gttcttcacc acccaggtat ttttccatca gctcttcaga agcttcagct
      601 gcggattcga tcaggttctg gtgccattcg ttagccagtt caaccatgtc tgccgggata
      661 tcttcgtatt cgaaggttac gccctggtca gcgtcgttcc agttgatagc tttcattttc
      721 accaggtcaa caacaccggt gaaatgttct tcagcaccaa tcgccagctg cagcggaacc
      781 gggttcgcgc ccagacgggt tttgatctgg ttaacaactt tcaggaagtt cgcacccatg
      841 cggtccattt tgttaacgaa cgcaatgcgc ggaactttat atttgtttgc ctgacgccat
      901 acggtttcag actgcggctg aacaccacca actgcgcagt aaaccattac cgcaccatcg
      961 agaacacgca tggaacgttc tacttcgatt gtgaagtcaa cgtgccccgg ggtgtcgatg
     1021 atgttgatgc gatgcggctc atactgctta gccataccag accagaatgc agtagtcgca
     1081 gcggaagtga tggtaatacc acgttcctgc tcctgctcca tccagtccat ggttgcagcg
     1141 ccgtcatgaa cttcaccgat tttatggttt acaccggtgt agaacagaat acgttcggta
     1201 gtagtggttt taccggcgtc gatgtgcgca ctgataccga tgttacggta gcgtgcgatg
     1261 ggtgttgtac gagccatttg tttcctcgtt tatcttttag gcgttcaatt taagtagccc
     1321 aaagcgggct gcttactgga agcgcccgcc tggtgactaa aactccgaag ggattaccaa
     1381 cggtagtgtg cgaacgcctt gttggcttcg gccatacggt gaacgtcttc acgtttctta
     1441 actgcagtac ctttgttttc tgcagcatca gaaagttcgt tcgccaggcg cagagccatg
     1501 gatttatcac cgcgtttacg agcagcttca acgatccaac gcattgccag agcattacga
     1561 cgaaccggac ggacttcaac tggtacctga taagtagaac caccaacgcg gcgagactta
     1621 acttctacag tcgggcgcac gttttcgaga gctacttcga atgcttccag ttcagattta
     1681 ccagagcgct gagccagggt ctccagcgcg ctgtatacga tagattcagc agtagatttt
     1741 ttaccatcta ccatcaggat atttacaaat ttagccagca gttctgatcc gaacttcgga
     1801 tccggcagaa ttttacgctg accaatgacg cgacgacgtg gcatggaaat actccgttgt
     1861 taattcagga ttgtccaaaa ctctacgagt ttagtttgac atttaagtta aaacgtttgg
     1921 ccttacttaa cggagaacca ttaagcctta ggacgcttca cgccatactt ggaacgagcc
     1981 tgcttacggt ctttaacgcc ggagcagtca agcgcaccac gtacggtgtg gtaacgaaca
     2041 cccgggaggt ctttaacacg accgccacgg atcaggatca cggagtgctc ctgcaggttg
     2101 tgaccttcac caccgatgta ggaagtcact tcgaaaccgt tagtcagacg aacacggcat
     2161 actttacgca gcgcggagtt cggtttttta ggagtggtag tatatacacg agtacatacg
     2221 ccacgttttt gcgggcatgc ttccagcgca ggcacgttgc ttttcgcaac tttgcgagca
     2281 cgtggtttgc gtaccagctg gttaactgtt gccattaaat agctcctggt tttagctttt
     2341 gcttcgtaaa cacgtaataa aacgtcctca cacaatatga ggacgccgaa ttttagggcg
     2401 atgccgaaaa ggtgtcaaga aatatacaac gatcccgcca tcaccaggcc atctggctgg
     2461 agtgcttaac cgtaagtctg acgaaatcag tatagtcaat gggaatgatg tcgttcgaaa
     2521 tttgaccagt caaaccgcgg gcaataaggt cttcgttcag ggcatagacc ttaatggggg
     2581 cattacgcag actttcaagg tagcggttac cgtcaactgc ggcagttacg ccatcttgca
     2641 ataatagcag ttcgtctcct tcactgagca gacgcagcag cgcagcaaaa tccgtcagcc
     2701 agggtgaacg atgtaacgtg tgcagcataa acgcctcaaa acctcaaaat aacatcgtag
     2761 ttggcgagtt cgcggcgtaa ggcatctgct tcgagcggcg tggcttcgac aacaaagggt
     2821 gtctgcggat ctaacccgcg ttcgcgcagt gaagccgcac aaacccagca ctgttcaatg
     2881 tcgtacagac ccaacaattt aaaagtggca atgtaatcac gcgccagcac tgcatcgggc
     2941 ttttgtcctg acagcagctg aaaaacgcca tcagcaataa agaagacggc cagatcgtca
     3001 gttaatgcgg aagttgccag taaagcatct aaaccttccc gaccagcagc tgtaccatga
     3061 ggtgcagtag aaaaaacaaa cgcaattcgt ttcatcagaa ctgtaccacc ctgtcacagg
     3121 tcagcgaggc ttccgccagc gcgccaagtc cgcttaaggt aaatcccggc tgaaggtttg
     3181 acgaagccag ccccagcctt cctgcttcag tttcatcgac gataccgcgg cgtaacgctg
     3241 ccgctacgca gatattcagc gccacaccat gttgcgcatt caactgttgc cagccgcgca
     3301 caaggtcaaa ttcgtcactt gccggtgagg tcaattggtt agcgttatag accccttccc
     3361 gatagaaaaa gacgctgctt aactcatggc cttctactat cagcgcctgc gcaaactgaa
     3421 aagcactact cgcctgttgc gtaccgtatg ctggcccggt caccacgatg gcaaaacgca
     3481 ttacttatct tgcccctgga aatcaccgct tttgaactgg cggatgtaga gatagacagt
     3541 gtgtttggag atgttcaggc ggtcagcaac ctggttgatc gcgtctttaa tatcgaagat
     3601 ccctttctcg tagagattca gcacgatctg acgatttttg gcgttattag aaacattgcg
     3661 atcggcattt acttcttcga tggtgaactc cagcgtttgg gtaaccagat cttcaacaga
     3721 agaggcaaag ttgacgcttg aaccgacatc cggggtttct ggcggcacaa aagtgctcat
     3781 aatctgcgag aagggaacat caagattcat attgatgcac agcagaccaa ttacacgctg
     3841 ttcgcggtta cgaatcgcga tagtcaggga cttcattaat acgccgcttt tggcgcgagt
     3901 aaagtagcat ttagaaacgc tgctatccgc tcccgtcata tcgtgcagca tacgtagcgc
     3961 caggtcagta attggcgaac caatcttccg gcctgtatgt tcaccgttag caatgcgaat
     4021 ggctgaacat tttagatcct gcaaagagtg caaaacgatt tcacagtggg agccaataag
     4081 catcgctaac ccgtccacca ccgcttcgta ggatttcaga atatcaaaat cggtctggtc
     4141 gaaaggacgt tgatccagta aatccaactc actggtttcg ttggttaaaa gcgacctgga
     4201 catgaaaaaa aacactcctt ttcaggagcc tgtcgttaac ttttcagggc aggctcatta
     4261 atgatgcggg taactaaatt aatacagcgg aggttccgct ttccagcact aattatatcc
     4321 ggcctgtaat aaaaaaaccg ccgcctggtc aggcggcggt tcttaatgct tattttttag
     4381 cagaatctgc ggctttcgca tcagcttccg gctttgcatc agccttcggc gctggtttca
     4441 cgtccaacag ctctacgtca aacaccaggg tagaattcgg tgggatcccc ggaacacccg
     4501 ctttgccgta agccagttct ggtggaataa ccagtttgat cttaccgcct ttcttgatgt
     4561 tcttcagacc ttctgtccaa cccgggataa caccgtccag acggaaagag agcggttcac
     4621 cacgagtgta agagttgtcg aactctttac cgtcgatcag agtacctttg tagttcacga
     4681 caacagtatc gctgtctttc ggtgcttcgc ctttaccggc ttctactacc tgataaacca
     4741 gaccagttga agaggttttc acacctttct ctttggcaaa tttctcgcgg tactctttac
     4801 cttttgcttc gttatcagcc gcgtcttttt ccatcttcgc ctgagcagaa gacttcacgc
     4861 gagcttcgaa tgcttgcaga gtctgttcga tctcttggtc ggagagtttg ctcttatcag
     4921 caaatgcatc ctgaacacca gcgatcagct gatctttatc cagtttgatg cccagttttt
     4981 cttgttcttt tagagagttt tccatgtaac gacccagcga ggcacccagt gcataagctg
     5041 atttctgatc gtcatttttg aacgctgctt tgctgtcagc agttgtagca ggttttgcag
     5101 cttcagcagc aaaagtgatt ggtgcatgca gggcaacggc cattgtggtc gccagcagcg
     5161 ttactttaaa cagtgatttc atccatatct ccaggatcgg ggcatctcac cccagggtta
     5221 actattatca gaagggtact ataaagcgtt gtcgaacaaa tctacataca gacacgccct
     5281 attatcatct attttcagac tctttttgtt taaattagtt tcgatgaccg cgaaatgagt
     5341 gctgtctcgg gcagcaaagt taagtagaat ccgcggcgac cattcgacaa aagaggtgaa
     5401 tcatgcagga tttatcattg gaaacacgcc tggcagagct ggagagccga ctggcttttc
     5461 aggaaatcac cattgaagaa ctgaacgtca cggtgaccgc tcatgaaatg gagatggcga
     5521 aactgcgcga tcatctgcgt ctgctgaccg agaagttaaa agccagccag ccgtcgaaca
     5581 tcgcgtcgca ggctgaagaa acgccaccgc cacattattg aggcgtaaaa aaagcgggga
     5641 ttccccgctt ttttgtcact ttttcggtat tagtggcaac cgcaaccgcc gttgccttta
     5701 ccgccacagc agccttcgcc accgtgttca tgaccgtgat cgtggccatg accgccgcag
     5761 caaccgtcgt ggtcgtgatc gtggtggtga tcgtgcgcgc cgtgaacgtg accatgagcc
     5821 agttcttctt cagtcgcttc gcgaatcgcc acaacttcaa cgttgaattt caggttctga
     5881 ccggccagca tgtggttacc atcaaccacg acgtgatcgt cttcaaccgc agtgatttca
     5941 accggtaccg gaccctggtc ggtttcagcc aggaaacgca tacctacctg cagttcatca
     6001 acgcccataa atacgtcttt aggaacacgt tgcaccaggt tttcgtcgta ctgaccgtaa
     6061 gcgtcgttcg cgccaacagc gacatcaaat ttgtcgccaa cttcatgacc ttccagcgcc
     6121 gtttccaggc cagagatcag ggaaccgtga ccatgcaggt agtccagcgg cgcactcacc
     6181 ggagactcat caaccaacac accgtcttct gtacgtacct gataggccag gctgaccacc
     6241 aggtcttttg ctactttcat gatatctcct gagcatggga agaatagtgg cgcagattgt
     6301 agcggaattc tgcagccgtg tactcactag cttaaaaaaa ctcggcgcat atcgctagtc
     6361 cggatgaaaa atcccgatca cttgctcatc tttgcgaacg tgatcgcggg cttctttgtc
     6421 tgcttctcgc atctgatgtc cgcacttaac acattcaaca atatcaatat tattttcgcg
     6481 ccacatcgcc attgaatcct gcgcctgaca ggccgggcat tttgcgcccg caataaaacg
     6541 ttttcggatt gccatcttta ccctctactc aaattcatcc cagccgtcca gctggcgtcg
     6601 ttcttgttgc atttcacgct ggaaaatctc ttccagttcg cgtcgggctt ccctggcgcg
     6661 agaaatttgt acggtatcgg catgcattgg gatgagctct cgcagcatcc gcatatccag
     6721 gcggcgaaaa tgcagttgcg ctcgctgtgc ctgatgcgga tgcatgccaa gcgtgaccag
     6781 cgtcttgcgc cccagctcta acgcactgga gaatgtttca cgggaaaact gcgtcacccc
     6841 tgcctgtaat aactcatgcg cttccacacg tccgcgcgct cgcgcaagaa tatgcaaatg
     6901 cggaaagtgc tgctggcata tttccaccag cttcatggtg tcttccggct cgttacaggt
     6961 aatgacgata gactcagcgg cctctgcacc cgcagaacgt aaaagatcga cctgcgtggc
     7021 gtcgccgtaa taaactttgt agccgtattt acgcatcagg ttaacggcgc tgatatcccg
     7081 ctccagcacg gtaatgcgca ttttatttgc catcagcaaa cgaccaatca cctgaccaaa
     7141 acgcccgaag cccacgacaa tgacctgggg tttatcatcg ttgacccacg gtttttcgtc
     7201 ttcttcttcc ggtccgttaa actggcggga tagccattta tccaccagct tcatcagcaa
     7261 cggcgtggtc atcatggaaa gcgtcaccgt caccagcaac aacgccatct ggtcgccctg
     7321 gaataagcgt tgtgaagaag cggtagaaaa gaggacaaag gcaaactcac caccctgact
     7381 caacacgcca gcaaactgca tccgctctga gctacgcacg ccatacaatc gcgccagcag
     7441 atacagcacg agaattttca ccgccaccag cacaaccacg cttatcacta cccacaacag
     7501 atgggtataa agcaccccga ggttgagtga catgccgaca gagataaaga acaaaccgag
     7561 cagcaagcct ttgaagggat cgatagccgt ttccagttca tggcgatatt cactttccgc
     7621 cagcagcacg cccgcaataa atgtaccgag cgccatcgac agccccagcg catccataaa
     7681 caatgcggaa cccaacacca gcagcagcgt cgcggcggtg aacacttccc gcacgccaga
     7741 agctgcaata aagcggaata ccggacgcag taaatagcgc ccaccaatca gcatgccgac
     7801 aaacgccagc accttcatgc cgatcttcat ccagtcgaaa tgttcgtctg ccgaccccgc
     7861 caacaacggc actaacgcca gtgctgggat tactgccaga tcctgaaaca gcagaaccga
     7921 aaacccgagc tggccggatt cgctgcgatt catccctttc tcacgcatca attgcaatgc
     7981 cattgcagtt gaagacatcg caaggccaat gccaccgacc accgccgcct gccaggcgaa
     8041 atccgtcagc atcaataatc ccgccagcaa cgccgcgctt aacagcacct gtgccgcgcc
     8101 tacgccaaaa atcgaacgcc gcagttgcca aagtttggag ggattcaact caaggccgat
     8161 gataaacatc aggaatacca cgccgagttc cgaaaagtgg aggatctcat cgacgtcgct
     8221 aataaacccc agcccccacg ggccaattgc aatccctgcc agcaaatatc ccaacacagc
     8281 gccaataccc agccgcgatg ccagcggcac cgcagccacc gccgcgaaga gaaacagcac
     8341 tcctgcgagt aaaaaatcgg aaccttccat cagcggcctc ctggagacag cggatttgcc
     8401 agccagtcac cgtaggctct ggcgtggctc gccagctcct gtgcgctttg ccgtctcgcc
     8461 cagtaaataa tgatgggact taaccaatgc atccggcaca tgcccgccgc cagttcaaag
     8521 gggcgcagca catcgctcat cgggtagcga ttcagcgcgt cataacggta agcactttct
     8581 ggctcgccgg tggtaatcac gctacgccag tactttcccg ccagttggtt tcctcccggc
     8641 ccgctggcaa aaccacgact taatacccgg tccagccact ctttcagtag cgccgggcag
     8701 ctataggtat aaagaggatg ctgaaagaca atcacctcgt gctcgcgcag taatgcctgc
     8761 tcacggggga tatcaataaa aaaatcggga tagtgcgcgt aaaggtcgtg cacggtaaca
     8821 ttgctgagct gcgtggccgg tttaagcagt acccggtttg ccaccgagtc ctgagattcc
     8881 ggatgggcat acagcagcaa aactttcgct ggctgagaca tcatccccct cccggtatgg
     8941 tttttgtgta tagtcgctgt tttgggctac cattgcgccc ggtgcggcag ctcgcccata
     9001 cattacatta tcataatgat aagttaacat agtctgaaca tacggcacct tatgattgtt
     9061 ttctcctcgt tacaaattcg tcgcggcgtg cgcgtcctgc tggataatgc caccgccacc
     9121 atcaacccag ggcagaaagt cggcctggtg ggtaaaaacg gctgtggtaa atctaccctg
     9181 ctggcattgc tgaaaaatga aatcagcgcc gacggcggca gctacacctt tccgggaagc
     9241 tggcaactgg cgtgggtgaa tcaggaaacg ccggcgttac cgcaagcggc gctggaatat
     9301 gtcattgacg gcgaccgtga atatcgtcaa ctagaagcgc agctacacga cgccaacgaa
     9361 cgtaacgacg ggcacgccat tgcgaccatt catggcaagc tggatgctat tgacgcatgg
     9421 agtattcgct cccgtgctgc cagcctgctg cacggcctcg gtttcagcaa tgaacaactg
     9481 gagcgcccgg taagtgattt ttccgggggc tggcgtatgc gtcttaacct tgcccaggcg
     9541 ctgatttgcc gttcagactt gctgctgctc gacgaaccga ctaaccacct cgatctcgat
     9601 gccgttatct ggctggaaaa atggttgaag agctatcagg gcacgctgat cctgatctct
     9661 cacgaccgcg acttcctcga tccgatcgtt gataaaatta ttcatatcga acaacaaagc
     9721 atgttcgagt acaccggtaa ctacagttcg tttgaagtac agcgcgccac ccgtctggcg
     9781 cagcaacaag cgatgtatga aagccagcag gaacgcgtag cgcatctgca aagttatatc
     9841 gaccgtttcc gtgccaaagc caccaaagcg aagcaggccc agagccgcat taagatgctc
     9901 gagcgtatgg agctgattgc ccccgcgcac gtcgacaacc cgttccgctt tagcttccgc
     9961 gcgccggaaa gcctgccaaa tccgttactg aagatggaaa aagtcagcgc gggctatggc
    10021 gatcgcatta ttctcgactc gattaaactg aacctggtgc ccggctcgcg cattggtctg
    10081 ttaggccgca acggcgcggg taaatcgaca ttaatcaaac tgttagccgg tgaacttgcg
    10141 ccagtcagcg gtgaaattgg tctggcgaaa gggatcaagc tcggctactt cgcccagcat
    10201 caacttgaat acctgcgcgc cgacgaatca cctattcaac atctggcacg tttagcgccg
    10261 caggagctgg aacaaaaact gcgtgactac ctcggcggct ttggtttcca gggcgataaa
    10321 gtaaccgaag aaacgcgccg cttctcaggt ggggaaaaag cccgcctggt gctggcatta
    10381 attgtctggc agcgtccgaa tctgctgctg ctcgacgaac cgactaacca ccttgacctc
    10441 gacatgcgtc aggcactcac cgaagcatta atcgagttcg aaggcgcgct ggttgtcgtt
    10501 tcgcacgacc gtcatttgct gcgttccacc actgacgatc tctacctggt tcacgatcgt
    10561 aaagtcgaac cgttcgacgg cgatctggaa gattatcaac agtggttgag cgacgtacaa
    10621 aagcaggaaa accaggccga cgaagcgcca aaagagaacg cgaacagcgc ccaggcacgt
    10681 aaagatcaga agcgtcggga agcggagttg cgtgcgcaaa cccagccact gcgtaaagag
    10741 attgcccgtc tggaaaaaga gatggagaag ctgaacgcgc aactggcgca ggcagaagag
    10801 aaactcggcg acagcgaact gtatgaccag agccgtaaag cggagttgac cgcctgcctg
    10861 caacagcaag ccagcgccaa atccggcctg gaagagtgcg aaatggcatg gctggaagcc
    10921 caggagcagc ttgagcagat gttgctggaa ggccaaagca actgatggcg cagataacga
    10981 cgaccgatgc caatgaattc agcagcagtg ctgaattcac ccctatgcgc ggctttagca
    11041 attgtcatct gcaaaccatg ctgccgcgtc tgtttcgtcg caaggtgaaa ttcaccccgt
    11101 actggcagcg gctggagttg cccgacggcg attttgtcga tctcgcgtgg agtgaagccc
    11161 ctgcacaggc tcggcataaa ccgcgtttag tggtatttca cgggctggaa ggcagtctca
    11221 atagccctta cgcccacggt ctggttgagg cggcgcaaaa gcgcggctgg ctgggcgtgg
    11281 tgatgcattt tcgcggatgc agcggtgaac caaaccgtat gcaccgcatt taccattcgg
    11341 gcgaaaccga agacgccagt tggtttttac gctggctgca acgcgaattt ggtcatgcgc
    11401 caacggctgc cgtcggctat tcgctcggcg gtaatatgct ggcctgtttg ctggcaaagg
    11461 aaggcaatga tctcccggtt gatgcggcgg tgattgtctc tgcgccgttt atgctggaag
    11521 cctgtagcta tcatatggaa aagggctttt cccgcgttta tcagcgttac ttgctgaacc
    11581 tgttaaaagc caatgccgcg cgcaagctgg cagcctaccc cggaacgctg ccgattaatc
    11641 tcgcgcagtt aaaatcggta cgtcgcatcc gtgaatttga cgatctaatc accgccagaa
    11701 ttcacggcta cgccgacgct atcgactatt atcgtcagtg tagcgccatg ccgatgctga
    11761 accggatcgc caaaccgacg ctgattattc acgccaaaga cgatccgttt atggatcatc
    11821 aggtgatccc gaaaccggaa agtctcccct cgcaggtgga gtatcaactg actgaacatg
    11881 gcggtcatgt tggctttatt ggcggtacat tacttcatcc gcaaatgtgg ctggagtcac
    11941 gcattcctga ctggttaaca acgtatctgg aggcgaaatc atgttgattc cgtggcaaga
    12001 cctctccccc gaaacgctgg aaaatttaat tgaaagcttt gtgttacgtg aaggcaccga
    12061 ttatggtgaa catgagcgta cacttgaaca gaaagtcgcc gacgtcaaac gccagctaca
    12121 gtgcggagaa gcggtgctgg tatggtcgga gctgcacgaa acggtcaata ttatgccgcg
    12181 cagtcagttt cgcgaataac ctgcaaccgt cggcggacga gataataatg atagctaagt
    12241 catggagtta ctatgtctgc caaacatccg gtcattgcgg taacaggatc cagcggcgcg
    12301 gggaccacca ccaccagcct cgcgtttcgt aaaatattcg cgcagttaaa tctgcatgca
    12361 gctgaggtgg aaggcgacag ttttcaccgt tacacccgcc cggaaatgga catggcgatc
    12421 cgcaaagcgc gcgacgccgg gcggcatatc agctacttcg gccccgaagc gaacgacttc
    12481 ggcctgctgg aacaaacctt cattgaatac ggtcagagcg gcaaagggaa atctcgcaaa
    12541 tatctgcata cctacgacga agccgtaccc tggaatcagg taccgggaac attcaccccc
    12601 tggcaacctt taccagaacc cactgatgta ctgttttatg aaggtttaca cggcggcgta
    12661 gtcacgccac agcataacgt tgcgcagcat gtggacttac tggtcggcgt agtgcctatc
    12721 gttaaccttg agtggattca aaaactgatc cgcgacacca gcgagcgcgg gcactcacga
    12781 gaagcagtga tggactcggt agtgcgttca atggaagact atatcaacta catcacaccg
    12841 cagttttccc gcacccatct taacttccag cgcgttccca ccgtcgacac ttcaaacccg
    12901 ttcgcggcaa aaggtatccc gtcgctggat gaaagctttg tggtgatcca ttttcgtaat
    12961 ctggaaggga tcgatttccc ctggctgctg gcgatgttgc aaggctcatt catttcccac
    13021 atcaatacgt tagtggtgcc cggcggcaaa atgggtctgg caatggaatt aattatgctg
    13081 ccgctggtgc aacgattgat ggaaggaaag aaaatcgagt aactctgcta ttacgccgga
    13141 taaaatacta tccggcttca caacgggata gttaaatcac gcggcaacca cttcatacga
    13201 gtgagtaata ttcaccgctt tttccagcat caacgccact gaacaatatt tctcagcaga
    13261 gagatcaacc gcacgcgcaa ccgctgcgtc tttcaggtcg cgaccggtga cgataaaatg
    13321 cagattaatg tgcgtaaaca ggcgtggtgc ctcttcgcgg cgttcagagg tcaattttac
    13381 ttcacaatcg accacatcct gacgcccttt ttgcaggatc gaaaccacat cgatggcact
    13441 gcaaccaccc gccgccatca acaccatttc catcggactt ggtgctttat cgcctgagtt
    13501 gccgtccatt aaaatctgat gaccagaggc ggattcgccc agaaaagtta acccttcgac
    13561 ccacttcact cgcgcttgca tttttgctac tccactgcgt caattttcct gacagagtac
    13621 gcgtactaac caaatcgcgc aacggaaggc gacctgggtc atgctgaagc gagacaccag
    13681 gagacacaaa gcgaaagcta tgctaaaaca gtcaggatgc tacagtaata cattgatgta
    13741 ctgcatgtat gcaaaggacg tcacattacc gtgcagtaca gttgatagcc ccttcccagg
    13801 tagcgggaag catatttcgg caatccagag acagcggcgt tatctggctc tggagaaagc
    13861 ttataacaga ggataaccgc gcatggtgct tggcaaaccg caaacagacc cgactctcga
    13921 atggttcttg tctcattgcc acattcataa gtacccatcc aagagcacgc ttattcacca
    13981 gggtgaaaaa gcggaaacgc tgtactacat cgttaaaggc tctgtggcag tgctgatcaa
    14041 agacgaagag ggtaaagaaa tgatcctctc ctatctgaat cagggtgatt ttattggcga
    14101 actgggcctg tttgaagagg gccaggaacg tagcgcatgg gtacgtgcga aaaccgcctg
    14161 tgaagtggct gaaatttcgt acaaaaaatt tcgccaattg attcaggtaa acccggacat
    14221 tctgatgcgt ctgtctgcac agatggcgcg tcgtctgcaa gtcacttcag agaaagtggg
    14281 caacctggcg ttcctcgacg tgacgggccg cattgcacag actctgctga acctggcaaa
    14341 acaaccagat gctatgactc acccggacgg tatgcaaatc aaaattaccc gtcaggaaat
    14401 cggtcagatt gtcggctgtt ctcgtgaaac cgtgggacgc attctgaaga tgctggaaga
    14461 tcagaacctg atctccgcac acggtaaaac catcgtcgtt tacggcactc gttaatcccg
    14521 tcggagtggc gcgttactga gtagcgcgcc attttgtttc ccccgatgtg gcgcagactg
    14581 atttatcacc ccgatattaa ctatgcactg cggcaaacgc tggtgctctg tttgcccgtg
    14641 gccgttgggt taatgcttgg cgaattacga ttcggtctgc tcttctccct cgttcctgcc
    14701 tgttgcaata ttgcgggcct tgatacgcct cataaacgtt ttttcaaacg cttaatcatt
    14761 ggtgcgtcgc tgtttgcagc ctgtagcttg ctgacacagc tgctactggc aaaagatgtt
    14821 cccctgccct ttttgctgac cggattaacg ctggtacttg gcgtcactgc tgagttgggg
    14881 ccattgcacg caaaattgct tcccgcatcg ctgctggcag ccatttttac cctcagtctg
    14941 gcgggataca tgccggtctg ggaaccgttg ctcatctatg cgttgggcac tctctggtac
    15001 ggattgttta actggttttg gttctggatc tggcgcgaac aaccgctgcg cgagtcacta
    15061 agtctgctgt accgtgaact agcagattat tgtgaagcca aatacagcct gcttacccag
    15121 cacaccgacc ctgaaaaagc gctgccgccg ctgctggtgc gccagcaaaa agcggtcgat
    15181 ctaattaccc agtgctatca gcaaatgcat atgctttccg cgcaaaataa tactgactac
    15241 aagcggatgc tgcgtatttt ccaggaggcg ctggatttac aggaacatat ttcggtcagt
    15301 ttgcatcagc cggaagaggt gcaaaagctg gtcgagcgta gccatgcgga agaagttatc
    15361 cgctggaatg cgcaaaccgt cgccgctcgc ctgcgcgtgc tggctgatga cattctttac
    15421 catcgcctgc caacgcgttt tacgatggaa aagcaaattg gcgcactgga aaaaatcgcc
    15481 cgccagcatc cggataatcc ggttgggcaa ttctgctact ggcatttcag ccgcatcgcc
    15541 cgcgtgctgc gcacccaaaa accgctctat gcccgtgact tactggccga taaacagcgg
    15601 cgaatgccgt tacttccggc gctgaaaagt tatctgtcac taaagtctcc ggcgctacgc
    15661 aatgccggac gactcagtgt gatgttaagc gttgccagcc tgatgggcac cgcgctgcat
    15721 ctgccgaagt cgtactggat cctgatgacg gtattgctgg tgacacaaaa tggctatggc
    15781 gcaacccgtc tgaggattgt gaatcgctcc gtgggaaccg tggtcgggtt aatcattgcg
    15841 ggcgtggcgc tgcactttaa aattcccgaa ggttacaccc tgacgttgat gctgattacc
    15901 accctcgcca gctacctgat attgcgcaaa aactacggct gggcaacggt cggttttact
    15961 attaccgcag tgtataccct gcaactgttg tggctgaacg gcgagcaata catccttccg
    16021 cgtcttatcg ataccattat tggttgttta attgctttcg gcggtactgt ctggctgtgg
    16081 ccgcagtggc agagcgggtt attgcgtaaa aacgcccatg acgctttaga agcctatcag
    16141 gaagcgattc gcttgattct tagcgaggat ccgcaaccta cgccactggc ctggcagcga
    16201 atgcgggtaa atcaggcaca taacactctg tataactcat tgaatcaggc gatgcaggaa
    16261 ccggcgttta acagccatta tctggcagat atgaaactgt gggtaacgca cagccagttt
    16321 attgttgagc atattaatgc catgaccacg ctggcgcggg aacaccgggc attgccacct
    16381 gaactggcac aagagtattt acagtcttgt gaaatcgcca ttcagcgttg tcagcagcga
    16441 ctggaatatg acgaaccggg gagttctggc gatgccaata tcatggattc gccggagatg
    16501 cagccgcacg aaggcgcggc aggtacgctg gagcagcatt tacagcgggt tattggtcat
    16561 ctgaacacca tgcacaccat ttcgtcgatg gcatggcgtc agcgaccgca tcacgggatt
    16621 tggctgagtc gcaagttgcg ggattcgaag gcgtaatgca ggctaaacca tcggataagg
    16681 cattcacgcc gcatccgaca tcttttgcct gatgcttcgt atttacgccc caaccacctt
    16741 cgccaccgcg tgggcgaaac gttgcatccc ttcatcgata tccgcatctt ccaccaccag
    16801 cgacggtgca aaacgcatca catccggtcc ggcattcagc accattacgc cagcccctgc
    16861 gcccgcatac aggaaatcac gcgcccgacc tttgaactgt ggcttaagct ccgcgccaat
    16921 caacagcccc ataccgcgaa tatcgctaaa cacatcgtac tgctgatcga tcttctgcaa
    16981 atggtcaaca aaacgctggc gtttcgcctg aatgccttcc agcacttcag gggtattgat
    17041 gatatcaaac gccgcgcccg ctactgcaca ggccagagga ttaccgccgt aggtggaacc
    17101 gtgagaacca ggatgaaacg cagaagcaat ttccgccgtg gtcagcatgg cgctaatcgg
    17161 gaagccgccg cctaacgctt tcgcagaggt cagaatatcc ggcgtcacgc cgtagtgcat
    17221 gtaagcaaac aaatcgccgg tccgccccat cccgcactgc acttcatcaa acaccaataa
    17281 tgcctgatgt tgatcgcaca gctcgcgcaa gccctgcaaa aactctggcg tcgcagccgt
    17341 cacgccgccc tcgccctgga tcggctcaac caccaccgca caggtgtgat catccatcac
    17401 tgctttcact gcgtggagat cgttaaaggg aacgtggatg atgtctgccg gtttcggccc
    17461 aaagccgtcg gaatattttg gctgcccacc caccgaaacg gtaaacagcg agcgaccatg
    17521 aaaagcgtta tggaaggcaa taattttggt tttgaacggg ctatgacgca cacaggcgta
    17581 atgacgtgcc agtttaaagg cggtttcgtt agcttccgtg ccggagttca taaacaccac
    17641 gcgttcggca aacgttgcct caatcagttt acgcccaaga cgcagcgccg gttcattggt
    17701 gaaaacgtta ctgatatgcc acagagtttc gccctgggtt tttaacgcgt tcaccagcgc
    17761 aggatggcaa tggcccaacg ccgtaactgc aatgccaccc gcgaaatcga catactcctt
    17821 gccttgctga tcccagattc ggctgccctg accttttacc ggaataaact ctgccggagc
    17881 ataaatcggc aggatcactt catcgaaagt cgcgcgtgta attgctgttt gttcaattgc
    17941 catctcataa tcaccctgtt acgcataaat aaatgtgaaa ttataatcac aaaatatgca
    18001 taaaaaatca ctaaatggca atcagaaatc agcgatgcag gaaattagcc agcagttgat
    18061 gtccttgttc gctaagaata ctttctggat ggaactgcac accttccaga tcccactggc
    18121 gatggcgaat ccccataatc tcgcgggttt cgctccaggc cgtcacgtca aagcacgctg
    18181 gtaatgagtc aggttccacc accagcgaat ggtagcgtgt cacggtaagt ggatttgcca
    18241 gcccccgaaa tacgccctca ccgttatgtg taatcggcga ggttttgccg tgcatgacct
    18301 ttgcggcgcg cacaacttta ccgccaaatg cctgcgccat tgcctgatga ccgaggcaga
    18361 cgccaagaat cggcaagcgc ccggcatagt ggcgaataac gtcaagggag atcccggctt
    18421 catctggcgt acaggggcca ggtgagatga caattttttg tggtttaagg gcgtcgatat
    18481 ccgccagcgt caacgcatcg ttgcgcttaa ccagcacatc cgcccccagt tcacaaaagt
    18541 actggtagag gttccaggta aaagaatcgt agttatctat aagcaggatc atggcggctc
    18601 cggtacgaaa gaaccacgct attttactca gattccccgg cttcgcttac cactttgcta
    18661 aatatcatct gcagcgcggt gagatccccc attgccccac tctgatttgc ctgattccag
    18721 gcttcttttt cgataccctg ccagctcagt tgatatccgg catgaatcgc cagttgctcg
    18781 aagaagatcc gctgtgccag tccgcttccc acacggaagg gatgcagcac gttgatttca
    18841 caatagtaat gcgccagccg ctcgacgaac ttcgctttct ccaggccaac cagataacct
    18901 tcttcctcca gatcctgcat cagggcattg ccctcttttt cgatataagc aaagtggcag
    18961 aacggcgtat cgccctgata aatatcaact tcacgcagtt gccccgccca gtcgaaaata
    19021 tcctgataca gctggcgatg gatagtgcgc aaatgcggta aaccgcgcac cagcggacca
    19081 agctcaatgg tcgcagcacg cagcgccgtc atttcgtaag cggcctgttc cagccgctgc
    19141 tgctggtgga tgttcagccg gttacgcatg atatcaaggc caggataaag atacggatcg
    19201 cgcccttcgc cgaatttatc gctcatagtg cctcctcaac tcttcaaggc gcgctaaagc
    19261 ctctgcggca gtaagagtga ctaaaggcat ctcgacgcct tcaagacggc gactggcctg
    19321 gaaattacga ttacgctgaa gctcccagag acgggacttt tgcttatcgg tgagtttctt
    19381 cacttgatgc ctccctgaac gtgtccattt gccacaagta taagcggcaa atccgggtta
    19441 cgccgggaga gcagaagcgc gggcaagatt gcccgcgcga aaaatcatta cggcaggact
    19501 ttagcggaaa ggataactac cggttttgac ggcacattct ggtacggacc aacgtcatga
    19561 gtcggcacct gggaaatctt atcggcaacg tccatgcctt tcaccacttt accaaatacc
    19621 gcgtaaccga aatcacgctg accatggtca aggaaggcgt tatcggcaac gttgataaag
    19681 aactggctgg tggcgctgtc tttgtcagcg gtacgcgcca tcgcgatggt gccacgcgtg
    19741 ttgcgcaggc cgttatcggc ttcatttttg attggcgggt ttggtttttt ctgctgcatc
    19801 tgctcggtga aaccgccgcc ctgaatcata aagccaggaa tgacgcggtg aaaggtagtg
    19861 ttgttataaa aaccgctgtt cacataatcg acaaagtttt gcacagacac tggcgctttt
    19921 tgtttatcca gctccagttc gatgttacca gctgaggttg tcaacaatac gtgcgggtcc
    19981 cctttcgctg ccattgctgc gggagaaaga gcagaaagag cgaaaacagc agccatcgcc
    20041 gccagggtcg atttgaacat gagatttcct taacaaacag agcagaaaaa aagcaagtga
    20101 agagattcta aatagccttc aagaacaagg ccatcccttt acctaatttt acgtatctga
    20161 aatatatgta acctgacgat cactttttat tccgtgatca aaatcacctc ttaaaatgca
    20221 atttagcaac cgattgcaat aaaacattta aacagatcac aaaatcacct aaaatcgccc
    20281 gctcgcggca tatcccgcta tgccatttta tttttgaacg caggccaatc atgactaaca
    20341 gcaatcgcat caagctcaca tggattagct ttctctccta cgcactgact ggtgcgttgg
    20401 ttattgtcac cgggatggtg atgggaaata tcgccgatta tttcaatctg cctgtttcca
    20461 gtatgagtaa caccttcacc ttcctcaacg ccggcatttt aatctctatc ttcctcaacg
    20521 cctggctgat ggaaatcgtc ccgttgaaaa cgcagttacg ttttggcttt ctcctgatgg
    20581 tgctggcggt tgccggtttg atgttcagcc acagcctggc gctgttctcg gcggcgatgt
    20641 tcattctcgg ggtggtcagc ggcatcacca tgtcgattgg tacattcctg gtaacacaaa
    20701 tgtatgaagg gcgtcagcgc ggttcccgcc tgttatttac cgactccttc ttcagtatgg
    20761 ctgggatgat tttcccaatg atcgccgcgt ttctactggc gcgcagcatt gagtggtact
    20821 gggtttatgc ctgcatcggg ctggtgtatg tcgctatttt tattctgacc ttcggctgtg
    20881 agttcccggc gctgggcaaa catgcgccaa aaacggatgc tccggtagag aaagaaaagt
    20941 gggggatcgg cgtactgttt ctctctgttg cggcgctgtg ctacatcctc ggtcagttag
    21001 gttttatctc ctgggtgcct gagtatgcca aaggcctggg catgagcctg aacgacgcgg
    21061 gcacgctggt gagtaacttc tggatgtcat acatggtcgg catgtgggcg ttcagcttta
    21121 ttcttcgctt ctttgatttg caacgcattc tgaccgtact ggctggtctg gctgcgattc
    21181 tgatgtacgt ctttaacacc ggaacaccag cacatatggc gtggtcaatt ctcgctctgg
    21241 gcttcttctc cagcgcgatc tataccacca tcatcaccct gggttcacag cagaccaaag
    21301 taccgtcgcc aaaactggtt aactttgtcc tgacctgcgg aaccatcggt actatgttga
    21361 cctttgtggt taccggcccg attgttgaac atagcggtcc gcaggcggca ctgcttacag
    21421 caaacggtct gtacgctgtc gtctttgtga tgtgcttcct gttaggtttc gtcagccgtc
    21481 accgtcagca taacaccctg acctctcatt aattgctcat gccggacggc actctcgtcg
    21541 tccggccttt tcctctcttc ccccgctacg tgcatctatt tctataaacc cgttcatttt
    21601 gtccattttt tgcacaaaca tgaaatgtca gacaattccg tgacttaaga aaatttatac
    21661 aaatcagcaa tatacccctt aaggagtata taaaggtgaa tttgatttac atcaataagc
    21721 ggggttgctg aatcgttaag gtaggcggta atagaaaaga aatcgaggca aaaatgagca
    21781 aagtcagact cgcaattatc ggtaacggta tggtcggcca tcgctttatc gaagatcttc
    21841 ttgataaatc tgatgcggcc aactttgata ttaccgtttt ctgtgaagaa ccgcgcatcg
    21901 cttatgaccg cgtacacctc tcgtcttact tctctcacca caccgccgaa gagctgtcgc
    21961 tggtgcgcga aggcttctac gagaaacacg gcatcaaagt tctggtcggc gaacgcgcta
    22021 tcaccatcaa ccgtcaggag aaggtgattc actccagcgc cgggcgcacc gttttttatg
    22081 acaagctgat catggcgacc ggttcctacc cgtggatccc gccaatcaaa ggttctgata
    22141 ctcaggactg ctttgtctat cgcactattg aagacctcaa cgccattgaa tcctgcgccc
    22201 gtcgcagcaa acgcggtgcc gttgttggtg gcggcctgtt aggtctggaa gccgctggcg
    22261 cgctgaaaaa cttaggtatt gaaacccacg ttatcgaatt tgcccctatg ctgatggcgg
    22321 aacagcttga tcagatgggt ggcgagcagc tgcgtcgcaa aatcgaaagt atgggcgtgc
    22381 gcgttcacac cagcaaaaac acccttgaga ttgtgcagga aggtgtcgaa gcgcgtaaaa
    22441 ccatgcgttt tgccgacggc agcgaactgg aagtcgactt tatcgtcttc tctaccggta
    22501 tccgtccgcg cgataagctg gcaacccagt gtggtctgga cgttgctccg cgtgggggta
    22561 tcgtcattaa tgattcctgc cagacttccg atccggatat ctacgccatc ggtgaatgcg
    22621 caagctggaa caaccgtgta tttggtctgg tagcacctgg ctacaaaatg gcgcaggtcg
    22681 ccgttgacca tattctcggt agcgaaaacg cctttgaagg tgctgacctt agcgccaagc
    22741 tgaaactgct gggcgtagac gtaggcggta ttggtgatgc gcacggtcgc acgcctggcg
    22801 cacgtagcta cgtttacctc gacgaaagta aagagatcta caaacgcctg attgtcagcg
    22861 aagacaacaa aaccctgctc ggtgcggtac tggtgggcga taccagcgac tacggcaacc
    22921 tgctgcaact ggtgctgaat gctatcgagc tgccggaaaa cccggattct ctgattctgc
    22981 cggcgcactc gggtagcggt aagccgtcta tcggtgttga taaactgccg gacagcgcgc
    23041 aaatctgctc ctgcttcgac gtcaccaaag gtgatctgat tgctgccatc aacaaaggct
    23101 gccacaccgt tgcggcgctg aaagctgaaa ccaaagcggg tactggctgc ggtggctgta
    23161 tcccgctggt tactcaggta ctgaacgcgg aactggcgaa acagggcatc gaagttaaca
    23221 acaacctgtg cgaacacttt gcttattcgc gtcaggaact gttccatttg atccgcgttg
    23281 aaggcattaa aaccttcgaa gaactgctgg cgaaacacgg caaaggctac ggttgtgaag
    23341 tttgtaaacc gaccgtcggt tcgctgctgg cctcctgctg gaacgaatac attctgaagc
    23401 cggaacatac tccgctgcag gattctaacg acaacttcct cgctaacatc cagaaagacg
    23461 gcacctactc ggtgatcccg cgttctccgg gcggtgaaat caccccggaa gggctgatgg
    23521 cagtgggtcg tatcgcgcgt gaatttaatc tctacaccaa aatcaccggc tcccagcgcc
    23581 tggcgatgtt tggcgcacag aaagacgatc tgccggaaat ctggcgtcag ctgattgaag
    23641 ctggcttcga aaccggtcat gcctatgcga aagcactgcg tatggcgaaa acctgcgtgg
    23701 gtagcacctg gtgccgctac ggtgttggcg atagcgtcgg cctcggcgtg gaactggaaa
    23761 accgctacaa aggcatccgt acgccgcaca aaatgaagtt cggtgtctcc ggctgtaccc
    23821 gtgaatgttc agaagctcag ggtaaagacg tgggtattat cgccactgaa aaaggctgga
    23881 acctgtatgt ttgcggtaac ggcggcatga aaccgcgtca tgcggatctg ctggcagcgg
    23941 atatcgatcg cgaaacgctg atcaaatatc tcgaccgctt catgatgttc tacatccgta
    24001 ccgccgacaa actgacgcgt accgcgccgt ggttagaaaa cctcgaaggc ggcatcgatt
    24061 acctgaaagc ggtgatcatt gacgacaaac tggggctgaa cgctcatctg gaagaagaga
    24121 tggcgcgcct gcgtgaagcg gtagtgtgtg agtggactga aacggtcaat acaccgtccg
    24181 cgcagactcg cttcaaacac ttcatcaaca gcgacaagcg tgacccgaac gtgcagatgg
    24241 tgccagagcg cgaacagcac cgtccggcaa cgccgtatga acgtatcccg gtaactctgg
    24301 tggaggacaa cgcatgagcc agtggaaaga catctgcaaa atcgatgaca tcctgcctga
    24361 aaccggcgtc tgcgcgctgt taggcgacga gcaggtcgcg attttccgcc cgtatcacag
    24421 cgaccaggtg tttgcgatca gcaacatcga tccgttcttc gagtccagcg tgctgtcacg
    24481 cgggctgatt gcggaacacc agggcgagct gtgggtcgcc agcccgctga aaaaacagcg
    24541 ttttcgctta agtgatggct tgtgcatgga agacgaacag ttttccgtca aacattacga
    24601 agcacgagtg aaagacggcg tggtgcagct gcgcggttaa tgttttaacg ggaggcgcaa
    24661 tgcctcccct ttttgcatgg tcctgtaatg accttcggta tattgcagga cattttttaa
    24721 actttttgtt ttattttttt gtttttattt tttaaaggat aatcaaatgt ttacagacac
    24781 tattaataag tgtgcggcta acgctgcgcg cattgcacgc ctgtcggcaa ataacccgct
    24841 cggcttttgg gtcagctccg ccatggcggg cgcgtatgtg ggtcttggga tcatcttgat
    24901 tttcacgctc ggtaatttgc tcgacccgtc cgtacgtccg ctggtgatgg gcgcgacctt
    24961 tggtatcgcc ttaacgctgg tgattatcgc cggttctgaa ctgttcaccg gacacaccat
    25021 gttcctcacc tttggggtaa aagcgggcag catcagccac gggcaaatgt gggcaatcct
    25081 gccgcaaacc tggctgggta acctggtcgg ttccgtcttc gttgccatgc tctatagctg
    25141 gggcggcggt agcctgctgc cggtagatac cagcatcgtt cactccgtcg cgctggctaa
    25201 aaccactgca ccggcaatgg tactgttctt caaaggtgca ttgtgtaact ggctggtttg
    25261 cctggcaatc tggatggcgc tgcgcactga aggggcggcg aaatttatcg ctatctggtg
    25321 gtgtctgctg gcatttatcg cgtccggcta cgagcactct atcgctaaca tgacgctgtt
    25381 cgcgctctcc tggttcggca accacagcga agcctacacg ctggcgggta ttggtcataa
    25441 cctgctgtgg gtgacgctgg gtaatacttt atcaggtgcc gtattcatgg gattgggtta
    25501 ttggtatgct acgccgaaag cgaatcgtcc ggttgcggac aaatttaatc aaactgaaac
    25561 ggctgccggt taattactaa ggggttttta cgtggatcat ttgcctatat tttgccaatt
    25621 acgcgatcgc gactgtctga ttgtcggcgg tggtgatgtc gcggaacgca aagcaaggtt
    25681 gctgttagac gcaggcgctc gcttaacggt gaatgcatta gcgtttattc cacagttcac
    25741 cgcatgggca gatgcaggca tgttaaccct cgtcgaaggg ccatttgatg aaagccttct
    25801 cgacacctgc tggctggcta ttgcagcgac ggatgatgac gcgcttaacc agcgcgtcag
    25861 cgaagccgct gaagctcgtc gcatcttctg taacgtggtc gatgcgccga aagccgccag
    25921 ctttattatg ccgtcgatta ttgaccgctc accgctcatg gtcgcggtct cctctggcgg
    25981 cacctctccg gttctggcac gcctgttgcg cgaaaaactc gaatcactgc tgccgttgca
    26041 tctgggccag gtagcgaaat acgccgggca attacgcggg cgagtgaaac aacagttcgc
    26101 cacgatgggt gagcgtcgcc gtttctggga gaaattgttc gttaacgacc gcctggcgca
    26161 gtcgctggca aacaacgatc agaaagccat tactgaaacc accgaacagt taatcaacga
    26221 accgcttgac catcgcggtg aagtggtgct ggttggtgca ggtccgggcg atgccgggct
    26281 gctgacgctg aaaggactgc aacaaattca gcaggcagat gtggtggtct acgaccgtct
    26341 ggtttctgac gatattatga atctggtacg ccgcgatgct gatcgcgttt tcgtcggcaa
    26401 acgcgcggga taccactgcg taccccagga agagattaac cagatcctgc tgcgggaagc
    26461 gcaaaaaggc aaacgcgtgg tgcggctgaa aggtggcgat ccgtttattt ttggccgtgg
    26521 tggcgaagag ctggaaacac tgtgcaacgc gggtattccg ttctcggtgg ttccgggtat
    26581 taccgcagct tctggttgct ctgcctattc gggtattccg ctcacgcatc gcgattatgc
    26641 ccagagcgtg cgcttaatta ccggacactt aaaaactggt ggcgagctgg actgggaaaa
    26701 cctggcggca gaaaaacaga cgctggtgtt ctatatgggg ttgaatcagg ccgcgactat
    26761 tcagcaaaag ctgattgaac acggaatgcc aggcgaaatg ccggtggcaa ttgtcgaaaa
    26821 cggtacggca gtcacgcagc gcgtgattga cggtacgctc acacagctgg gagaactggc
    26881 gcagcaaatg aacagtccat cgctaattat tattggtcgg gttgttggcc tgcgcgataa
    26941 actgaactgg ttctccaacc attaatttaa cccgggccag agaattctgg ccttcttaat
    27001 gacttttttt atatattcag caaataaaac ataacccctt aataaatatt atctgctaat
    27061 gggttatatt caccgttaca gcgacttaca aaattaagtc attcgctcac tgacgccaat
    27121 taaatattcc tgtattgttc ttaatgagca gtctgcttat attcatataa tcaatgaata
    27181 ttaattaata tataatacat agggaatgta aatgaacaaa tttattaaag ttgcactggt
    27241 aggtgcagta ctggctacgt taactgcatg tactggtcat attgaaaacc gtgataagaa
    27301 ctgctcttac gactacctgc tgcacccggc aatttctatt tctaaaatca ttggcggttg
    27361 cggtcctact gcacagtaag tctctgacag aaatcggcta acaccggttt aattgcccga
    27421 cgccctctgg attttccgag ggcgtatttg tttctatgtc cttcttgccc ccgcccgtaa
    27481 aaatattttc actttaaatt caatttgata actacatcca ttagccacag cgcgatcctg
    27541 tgcgaaattt tgtgatcttc ctccacatta cataacatca tatgttgtta tattcatcat
    27601 gcattgtcat gttacctttt aaatgactgc aaactctccc ctacaacgta ttggacaaga
    27661 aaaaggtatc gctatgggaa gccaggaact ccaacgcaag ctcggatttt gggccgttct
    27721 tgcaatcgcc gtcgggacaa ccgtcggctc cggtattttt gtatctgtgg gtgaagtggc
    27781 aaaagcagcg ggcacgccgt ggcttacggt gctcgcgttt gtcattggcg ggttaattgt
    27841 gatcccgcaa atgtgcgtct atgcggaact atccaccgct tatccggaaa atggcgcaga
    27901 ttatgtttat ctgaaaaatg ccggaagccg accgctggct ttcctctccg gctgggccag
    27961 cttctgggcc aacgatgcgc cgtcattgtc gattatggcg ctggcgattg tcagcaatct
    28021 tggcttttta acgcctatcg atccgttgct cggtaaattt atcgccgccg gattaattat
    28081 cgcctttatg ttgctacacc tgcgctccgt tgaaggcggc gcagcgtttc agacgctaat
    28141 taccatcgcc aaaattatcc cgttcactat cgtcattggc cttgggatct tctggtttaa
    28201 agcggagaat tttgccgccc ctaccaccac tgcgattggc gcaacgggca gctttatggc
    28261 gctgctggcg gggatctctg ccaccagttg gtcgtatacc ggcatggcct ctatctgtta
    28321 tatgaccggc gaaattaaaa accccggaaa aaccatgcca cgagcgctga ttggttcctg
    28381 tctgctggtt ctggtgctct acaccctgct ggcgctggtg atttccggcc tgatgccctt
    28441 cgacaaactc gccaattctg aaacgccgat ttccgacgcc ctgacctgga tccccgcact
    28501 cggcagcacc gctgggatct ttgttgccat cacggcgatg atcgtcattc ttggttcgct
    28561 ttccagctgc gtgatgtacc agccgcggct ggaatacgcg atggcgaaag acaacctgtt
    28621 ctttaaatgc ttcggccatg tgcatccgaa atacaacacg ccggatgtct ccatcatcct
    28681 gcaaggggcg ctggggatct tcttcatctt cgtttccgat ctcaccagcc tgctgggtta
    28741 tttcaccctg gtgatgtgtt tcaaaaatac cctcaccttc ggctccatca tctggtgtcg
    28801 taaacgcgac gattacaaac cgctgtggcg tactccggct ttcgggctga tgaccaccct
    28861 cgccattgcg tcaagcctca ttctggtcgc ctcaaccttt gtctgggcac cgattcccgg
    28921 ccttatctgc gccgtcatcg ttattgctac tggtctgcct gcttacgcct tctgggcgaa
    28981 gcgtagccgc cagctcaacg ctttgtcgta atgttatctg gagaaaataa aatgttggat
    29041 attgataaaa gcaccgtgga ctttctggtc accgaaaata tggttcagga agtggaaaaa
    29101 gttctcagcc atgacgttcc gctggtgcac gccatcgtgg aagagatggt gaagcgcgac
    29161 attgatcgta tttatttcgt tgcctgcggc tcgccactca acgcggcgca aacggcgaaa
    29221 catctggcgg atcgcttttc cgatcttcag gtctacgcca tttccggctg ggagttctgc
    29281 gataacaccc cgtatcgcct cgacgatcgt tgcgcggtga ttggcgtttc tgactacggt
    29341 aaaacggaag aggtaatcaa agcgctggag ctgggccggg cctgcggcgc actcactgcg
    29401 gcgttcacca aacgcgcgga cagcccgatt acctcggcgg cggaatttag cattgattat
    29461 caggccgact gtatctggga aattcacctg ctgctctgct acagcgtggt gctggagatg
    29521 atcacccgcc tcgcgccgaa cgcggaaatc ggcaagatca aaaacgatct caagcagttg
    29581 ccgaatgcgc tcggtcatct ggtacgcacc tgggaagaaa aaggccgcca gcttggtgaa
    29641 ctggccagcc agtggccgat gatttatacc gttgctgcgg gtccgctgcg tccgctgggt
    29701 tacaaagaag gcattgtaac gctgatggaa tttacctgga cgcacggctg cgtgattgag
    29761 agcggagagt tccgccatgg cccgctggag attgtcgaac cgggcgttcc gttcctgttc
    29821 ctgctcggca atgatgaaag tcgccacacc accgaacgcg ccattaactt tgttaaacag
    29881 cgtactgaca acgtgatcgt catcgattac gccgaaattt cgcaagggct gcacccgtgg
    29941 ctggcaccgt tcctgatgtt cgtgccaatg gagtggctct gctactacct gtctatttac
    30001 aaagatcaca acccggatga acgccgctat tacggtggtc tggtggaata ttaatccctc
    30061 tcccggcccg gcaacgggcc gaatttacgc aaggagttac ccgatgaaaa caggtatgtt
    30121 tacctgcggc caccagcggc tgcctattga acacgcattt cgtgatgcaa gcgagctggg
    30181 ttacgacggc atcgaaattt ggggcggtcg cccgcacgcg ttcgcgccgg acttaaaagc
    30241 gggcggcatc aaacaaatca aggcgctggc gcagacgtat cagatgccga ttatcggcta
    30301 tacgccagaa accaacggct atccgtataa catgatgctg ggcgatgaac atatgcgtcg
    30361 cgaaagcctc gacatgatca agctggcgat ggatatggca aaagagatga acgcgggtta
    30421 tacgctgatt tccgcggccc acgcgggcta tctcacgcca cctaatgtta tctggggacg
    30481 gctggcagag aacctaagcg aactgtgtga gtacgcggaa aacatcggca tggatctgat
    30541 cctcgaaccg ttaacgccgt atgaatcgaa cgtcgtgtgt aatgctaatg atgtgcttca
    30601 tgcgctggcg ctggtgcctt cgccgcgcct gttcagcatg gtcgacattt gcgcgccgta
    30661 tgtccaggcg gaaccggtga tgagttattt cgacaaactg ggtgataaat tgcgtcatct
    30721 gcatattgtc gacagcgacg gggccagtga cacacattac attcccggcg aaggcaaaat
    30781 gccgctgcgg gagctgatgc gcgatattat cgaccggggc tatgacggtt actgcacggt
    30841 ggaactggtg acgatgtata tgaacgagcc cagactctat gcccgccagg cgctggaacg
    30901 ctttcgcgcg ctgctgccgg aggatgagag atgaaaaccc tggcgacaat cggcgataac
    30961 tgcgtcgata tctacccgca actgaataaa gcgttttctg gcggtaatgc ggtcaatgtg
    31021 gcggtgtact gcactcgcta cggcatacag ccgggatgca ttacctgggt gggtgacgat
    31081 gactacggca caaagctgaa gcaggatctc gcccgcatgg gcgtcgatat cagccatgtc
    31141 catacgaaac acggcgttac cgcacaaact caggtggaac tgcacgacaa tgatcgcgtt
    31201 tttggcgact acaccgaagg cgtgatggcc gactttgccc tgagtgaaga ggattacgcc
    31261 tggctggcgc agtatgacat tgtgcacgcg gcaatctggg gacatgcgga agacgcattc
    31321 ccacagctgc acgctgcggg caaacttacc gctttcgact tctccgacaa gtgggacagc
    31381 ccgctctggc agacactggt gccgcatctc gattttgcct ttgcctccgc accgcaagaa
    31441 gacgaagcgc tgcgtctgaa gatgaaagcg attgttgccc gtggcgcagg aacagtgatt
    31501 gtcacgctgg gtgaaaacgg cagcattgcc tgggatggcg cgcagttctg gcgtcaggct
    31561 cctgaaccgg tgacggttat cgacaccatg ggtgccggag attcgttcat tgccggattc
    31621 ctttgcggct ggtctgcggg gatgacatta ccgcaggcga tggcgcaggg aacggcgtgc
    31681 gcggcgaaaa ccattcagta ccacggtgcc tggtaggtat aacgttggcg tgagcatctt
    31741 cacgccaacg tgctgttact tgccggaaaa cgaccctata atccgagtaa ttcattcttt
    31801 atttcagggt cgattatgtc agctacggac cgctactctc atcaactcct ctacgctacc
    31861 gtccgccagc gactgctgga tgatatcgcg cagggggttt accaggccgg gcaacagatc
    31921 cctaccgaaa acgagctttg tacacaatat aacgtcagcc gcattaccat tcgcaaagcc
    31981 atcagcgact tagtggcaga cggcgtactg atccgctggc agggaaaagg cacctttgta
    32041 caaagccaga aagttgaaaa cgccctgctt actgtcagtg gttttaccga ttttggcgtc
    32101 tcacaaggca aggcgacgaa agagaaagtg atcgaacagg aacgggtcag cgccgcgccg
    32161 ttttgcgaaa agctgaacat ccccggaaac agcgaagtgt tccatctctg ccgggtgatg
    32221 tatctcgata aagagccgct gtttattgat agttcatgga tcccgctgtc gcgttatcct
    32281 gactttgatg agatttacgt cgaaggaagc tccacctatc agttatttca ggagcgtttt
    32341 gacacgcgag tggtcagcga caaaaagacc atcgatatct ttgccgccac ccgcccgcag
    32401 gcaaaatggc tgaaatgcga actgggcgaa ccgttgtttc gcatcagcaa aatcgccttt
    32461 gatcagaatg acaaaccggt gcacgtctcc gaactcttct gccgcgccaa tcgcatcacc
    32521 ttaactattg ataataaaag acatta
//
LOCUS       DAMZET010000032        32157 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.032, whole genome shotgun sequence.
ACCESSION   DAMZET010000032 DAMZET010000000
VERSION     DAMZET010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.032"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..396)
                     /locus_tag="POB34_001633"
     CDS             complement(<1..396)
                     /locus_tag="POB34_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="activates the transcription of the ethanolamine
                     utilization operon; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutR"
                     /protein_id="HDI5918606.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELS"
     gene            complement(442..942)
                     /gene="eutK"
                     /locus_tag="POB34_001634"
     CDS             complement(442..942)
                     /gene="eutK"
                     /locus_tag="POB34_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HDI5918607.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(955..1614)
                     /gene="eutL"
                     /locus_tag="POB34_001635"
     CDS             complement(955..1614)
                     /gene="eutL"
                     /locus_tag="POB34_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDI5918608.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(1624..2511)
                     /gene="eutC"
                     /locus_tag="POB34_001636"
     CDS             complement(1624..2511)
                     /gene="eutC"
                     /locus_tag="POB34_001636"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HDI5918609.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(2532..3893)
                     /gene="eutB"
                     /locus_tag="POB34_001637"
     CDS             complement(2532..3893)
                     /gene="eutB"
                     /locus_tag="POB34_001637"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HDI5918610.1"
                     /translation="MKLKTTLFSNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETVYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(3905..5307)
                     /gene="eutA"
                     /locus_tag="POB34_001638"
                     /pseudo
     CDS             complement(3905..5307)
                     /gene="eutA"
                     /locus_tag="POB34_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
     gene            complement(5304..6530)
                     /gene="eutH"
                     /locus_tag="POB34_001639"
     CDS             complement(5304..6530)
                     /gene="eutH"
                     /locus_tag="POB34_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDI5918611.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            complement(6647..7834)
                     /gene="eutG"
                     /locus_tag="POB34_001640"
     CDS             complement(6647..7834)
                     /gene="eutG"
                     /locus_tag="POB34_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HDI5918612.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSEISVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(7824..8660)
                     /gene="eutJ"
                     /locus_tag="POB34_001641"
     CDS             complement(7824..8660)
                     /gene="eutJ"
                     /locus_tag="POB34_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HDI5918613.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGLRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(8671..10074)
                     /locus_tag="POB34_001642"
     CDS             complement(8671..10074)
                     /locus_tag="POB34_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HDI5918614.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(10086..10373)
                     /gene="eutN"
                     /locus_tag="POB34_001643"
     CDS             complement(10086..10373)
                     /gene="eutN"
                     /locus_tag="POB34_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HDI5918615.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(10480..10773)
                     /gene="eutM"
                     /locus_tag="POB34_001644"
     CDS             complement(10480..10773)
                     /gene="eutM"
                     /locus_tag="POB34_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HDI5918616.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(10812..11828)
                     /gene="pta"
                     /locus_tag="POB34_001645"
                     /pseudo
     CDS             complement(10812..11828)
                     /gene="pta"
                     /locus_tag="POB34_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
     gene            complement(11825..12628)
                     /gene="eutT"
                     /locus_tag="POB34_001646"
     CDS             complement(11825..12628)
                     /gene="eutT"
                     /locus_tag="POB34_001646"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HDI5918617.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(12625..13326)
                     /gene="eutQ"
                     /locus_tag="POB34_001647"
     CDS             complement(12625..13326)
                     /gene="eutQ"
                     /locus_tag="POB34_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HDI5918618.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(13301..13780)
                     /gene="eutP"
                     /locus_tag="POB34_001648"
     CDS             complement(13301..13780)
                     /gene="eutP"
                     /locus_tag="POB34_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HDI5918619.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(13793..14128)
                     /gene="eutS"
                     /locus_tag="POB34_001649"
     CDS             complement(13793..14128)
                     /gene="eutS"
                     /locus_tag="POB34_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HDI5918620.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(14421..16700)
                     /gene="maeB"
                     /locus_tag="POB34_001650"
     CDS             complement(14421..16700)
                     /gene="maeB"
                     /locus_tag="POB34_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HDI5918621.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            16989..17939
                     /gene="tal"
                     /locus_tag="POB34_001651"
     CDS             16989..17939
                     /gene="tal"
                     /locus_tag="POB34_001651"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDI5918622.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHTQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            17959..19962
                     /gene="tkt"
                     /locus_tag="POB34_001652"
     CDS             17959..19962
                     /gene="tkt"
                     /locus_tag="POB34_001652"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDI5918623.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(20058..21101)
                     /locus_tag="POB34_001653"
     CDS             complement(20058..21101)
                     /locus_tag="POB34_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HDI5918624.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(21227..21802)
                     /gene="nudK"
                     /locus_tag="POB34_001654"
     CDS             complement(21227..21802)
                     /gene="nudK"
                     /locus_tag="POB34_001654"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HDI5918625.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(21870..23849)
                     /gene="aegA"
                     /locus_tag="POB34_001655"
     CDS             complement(21870..23849)
                     /gene="aegA"
                     /locus_tag="POB34_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HDI5918626.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            24055..25755
                     /gene="narQ"
                     /locus_tag="POB34_001656"
     CDS             24055..25755
                     /gene="narQ"
                     /locus_tag="POB34_001656"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HDI5918627.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            25919..29032
                     /gene="acrD"
                     /locus_tag="POB34_001657"
     CDS             25919..29032
                     /gene="acrD"
                     /locus_tag="POB34_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HDI5918628.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(29131..29190)
                     /gene="ypfM"
                     /locus_tag="POB34_001658"
     CDS             complement(29131..29190)
                     /gene="ypfM"
                     /locus_tag="POB34_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HDI5918629.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            29571..29927
                     /locus_tag="POB34_001659"
     CDS             29571..29927
                     /locus_tag="POB34_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HDI5918630.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            29931..31058
                     /gene="dapE"
                     /locus_tag="POB34_001660"
     CDS             29931..31058
                     /gene="dapE"
                     /locus_tag="POB34_001660"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HDI5918631.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            31086..31286
                     /locus_tag="POB34_001661"
     CDS             31086..31286
                     /locus_tag="POB34_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HDI5918632.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(31396..32094)
                     /gene="ypfH"
                     /locus_tag="POB34_001662"
     CDS             complement(31396..32094)
                     /gene="ypfH"
                     /locus_tag="POB34_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDI5918633.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMVLESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
BASE COUNT         7328 a         8964 c         8418 g         7447 t
ORIGIN      
        1 gctcagttca aattcagtgc caccagggcg ggtggcgatt tccgcgcttc ccagacattg
       61 cgaaccgata aatccctgct caccgcgcgt cgccggaatg ccaaaccaga acgagttcgg
      121 ccagaccagg cacgactgac gcagcgccag accggtgtat tcacgaaaaa cctgaatatc
      181 atcgagtaga atttccgtaa attcaccatg aaacttgccc ggatgcagct gatcgtaaat
      241 ctgctgccag gcggtaatcg ttaaagcatg ttcatagaca tccgttgtct gtcgttgatg
      301 aacattatcc acttcgacct tcggcgtgag cttcaggttt tcgggtaagg gttcatgata
      361 aagatggtgc aaattggctg tacgggtctt tttcatgatg ttaatgccgg gtgttgtagg
      421 acacccggca cctccgacag gttaatgggg cttgagacga taacgactac tgcgtttacg
      481 taacgtcccg gcagaaaaga gctgttcgag cgcatttctg gctttttcca gcggccagcc
      541 aaagtgggca gccacttctc ctgccgtcat tccctgacgt actgatgtta acagcgccaa
      601 caactcgtca gcagattccg cgacgataac tggcgcgtcg ggttccctta cgggttgctt
      661 cggctggcgg ttaaagccag taaccagcca ctgggtgtca tcgtctggcc gaccgatctc
      721 cttgcggctg atgacacgcc cggtacgcat cgcggcagca caacctgcgt ccagcgccgc
      781 acgacacgcc gccagatcgc cttccaccac cagcgttaag cgaccagggt caagcacttc
      841 gtgactgagc agacgaacgt tagctgcttt gagcatggca tccgctgcat ctatcgcggc
      901 gaccatgccg tccacttcca gcaatcccag tgcattgatc atcggcaacc tccgttacgc
      961 acgctggatt ggattacgcg cgatttccag cactgcatcg gtaaaggcgt tacaggctgc
     1021 tttacacgcg gcctggctac cggttaaaaa tgctgccgag tagttggttt cagacggtgg
     1081 cgggacatag gtcaccagct gtacgtcggc agatttcaac gctgcatcaa tgccataggt
     1141 cgcttccagc ggcggtgcca ccagatacgc catcggatcg ccaagcgtga tcccggcggt
     1201 tgatgagaga taagaaccgg tacgcgaaac cacatgcgcc aggaaggcgg tgttttctgc
     1261 gtcattggcc cactggaatg ctgcaccatt ttcaatatgt gcaaccatcg catccagacc
     1321 ggcacgcact tccgccgggt ttggcccacc aagcataatc agcacttcac cggcagtcgg
     1381 tgacgggccg tgtgcagcgc ccgcatacag cgagcgacca tataccactt ccaccatcgc
     1441 ctgcttggtc gcttcatcag cggcaatgta ggtgacgtca tcggaatcag cagaaatcag
     1501 cccgaggcta cgaatatgcg gcggcaattt tagttcacgc gcaaattcag cgttaacgga
     1561 ggcaatcacc cgcatggcgg ttaccgacgg tcgaatcaaa tctaaagctg gcatgatgcc
     1621 tccttatcgg gtcatgttga tgccggatgc tttctgctcc agcatacgtt tcgccaaatc
     1681 cacaatgacg gctgcggctt caaccggcgg cgtgccgccc tggtgaatgt tagagataca
     1741 ggtgcgatcg gcctcaacgg tggtcgccat acgcggcgag taaacggcgt agcaggagag
     1801 gctttctgac tgccctaacc ccggacgttc ccccaccagc aggatcacca ctttcgcgcc
     1861 gaggatctca ccgatctgat cttcaatctt cacgcgacca taacgaacaa agaatggcgt
     1921 gccgactttc agcccggcct gtttcaggcc cgccatcagc ggcggcagga tctcttcgta
     1981 gttcacggtg atggcatcag ttgacaggcc atcagaaata acgacctgta cgtccggatt
     2041 agcaacacac tgtgctttca gcgcttcaac agcttctgca cacaggcggc ggcccatatc
     2101 cgggcgagtc aggtagagat ttttgtcgct aatttcagag cgtacttcca gcaagccctg
     2161 cgctttcacc cactcttccg gcacttcttt cagtacggta tctttcgaac gagagtgatc
     2221 ggccaggaaa cgcagcagcg cctgggtacg tggacgcgga ccggcacgac cggtacaaac
     2281 gcgggccacg gtgctgcggc gcagttctgt taatacgtct gcgcgatgcg ggttttcaac
     2341 accaatccac gcttttgctt cagcggaacc taaatccagc gcacagcttt ccgaggtcac
     2401 cggtgccgca cagttggtgg tggcgcactt tgcttctgac ggcgcggggg ccgtttgtcc
     2461 cattgacgcc atcacgctgc gtacaatttc ttcaatctgt ttttgatcca tgatgtgtta
     2521 tctccgcgtc atcagaagaa cagtgacgga tcgcccgccc gtttggtcag gcgaccgttt
     2581 gccataatgc ccatgctttc cagccagcgt tcaaactccg gtgacggacg caggttgagt
     2641 aactgacgca cagtggcggt atcgtggaat gcggtggtct ggtagttgag catgatgtca
     2701 tcacccagcg gcatccccat gatgtagttg cagcctgcgg tggcgagcag gatcatcagg
     2761 ttttcgttga ggttctggtc agcgtcagcg tggttggtgt aacagcagtc acagcccata
     2821 gagatgccgc tcagcttgcc cataaagtga tcttctaagc cagcacggat aatctggcgg
     2881 tcgttgtaga gatactccgg cccaataaag ccgaccacgg tgttgacgat aaacggatcg
     2941 taatgacgcg ccagcccata gttacgtgct tccatcgtca cctggtcagc gccgaagtta
     3001 gcgccagcgg atagcgcaga gccttgtccg gtttcgaagt agaggcagtt ttccccggcg
     3061 atacggttga actctgcacc cactgcgcgc gcttcgtcga gcatcgccag ttccacgcca
     3121 aactctttca gccctttttc gctgccacag atactctgga aaatcagtcc gccaggcgcg
     3181 ccgcgacgaa tcgcttcgat ctgggtggtg acgtgcgcca gtacgcagcc ctgagttggg
     3241 atgttgaatt tgtcgatcac gccgtaaatg gtgtccaaca cgcggcttaa gttttccacg
     3301 tcgtcagtca ccgggttaac gccgatcacc gcatcgccca ccccgaagga aagcccttcg
     3361 tagatttgcg cggcgatact ttgcacgtcg tcacgggtgt catttggctg caaacgggcg
     3421 ctgaaggtgc ccggaatacc aatggtggta ttggcctttt tgattaccgg cattttcttc
     3481 gcgccgtaga tcaggtccgc gttggagcaa atcttcgcta ccgccgcgac cacttccgag
     3541 gtcagccctt tgcgggtaaa ggcaatgtcg tccacgctgg tttcatcgct cagcacatac
     3601 tcacgcagtt cgctgatgct ccagttttta atctggttgt agaccgtttc gttaacatcg
     3661 tcctgaatca gccgcgtcac gcagtcatct tcataggcaa tcaccggatt attgcggatg
     3721 tccgctacgg tcatttccga caacacctgc tttgccgcca cgcgctcctg tgaacttgcc
     3781 gctgcaacgc ctgccagcac atcccccgaa cgcagttcgt tggctttagc cagcacctct
     3841 tttacatcct taaactgata tacattgctg aacaatgtgg tctttagttt cataagtcgt
     3901 tccctcagga aggaaatgcg agtgatttca ccgtcaccgg cacaaccgat ccgccaaaaa
     3961 gaggcgtacc aatgtcgata tagtcccccg cgcggacaat cacttcgtca atgactgcca
     4021 acgggagttg ttgtagctgt gggcgcaaca acatgcccag agctttacca aagtcctgcc
     4081 cggccaccac cagcagggga tgcggattcg gaaaacgcgc gacgaaatcg accagcgcgt
     4141 tgatgaccgt cagtaccgcg gcgtaacgca caggcagcga ggcgggaagc gccagcacgt
     4201 acgcgtcagt tttgggatca agatccagct gaagcagcgc ctgttgccag gcactcacca
     4261 gatccgtttc atcaatcggg atcgccaccg caaattgcgc agcggcagtt gtacgccctc
     4321 cagccagatt gtgctgccag agagcgaaag ggtatgtgca cccgcgccaa tcaccgtggc
     4381 gcgtacggtt tgcgccggaa actgcacatt catctcacgc aggcgcggat ggtcatgcag
     4441 cgccgttgcc agaagtgggc caatatcggc aaaacagaac gggtcggcgg gctggtggcg
     4501 ataacattcg cccacgcctc cagaaagcgt aatgatttcg ggcgtaacac ctgcgggcag
     4561 caaaccggtt tgcatcaatg cctgcgcgag cggcgaaagc gttccgtcaa tcacttcgac
     4621 aatcagcgcc gccatccgcc gggtaacctg caccagctgc gcgccggtca gcgaacgggc
     4681 gtcagtgcct gcaccgaagc actcatccac aatcatctgc cccggtttat gagcgtaaac
     4741 cacgcgcccc tggctgtcgg tttccagcag gcgaccaccg acgttgagac aggcagtgcc
     4801 gctgattttt ccggcatcga acagggcgta gttcgcggtg ccaccgccga tgtcgatatt
     4861 cagtacccga cacagccgtt gttcagaaag ggtttgtgcc ccggctccgt gaccggcgat
     4921 cacggattcg aggtgcggcc cggcgctggc aacgacaaaa tcgcccagcg actgagagag
     4981 cgccatcacc gccgggcgag cattgcgggt tttcgcgctt tcaccggtga tgatgatggc
     5041 accagaatca acgctttccg gcgcaatgcc cgcagcctga tattgctcta aaatcagcga
     5101 tttcagttcc gcttctttta aaccaccctg tttatcgaca ggggtaaaga ataccgggct
     5161 ttgccagcta atttcgcgtt taatgaattc gtagcgcggc acctgcgaca ccgccgcacg
     5221 gttaaccagt tccaggtggg agaaaatcac ctgggtggtg gtggtgccga tatcgatacc
     5281 gacgctcaat agctggcgag tgttcacgat tgtgcctccg cttcggtttt agtcgcggtc
     5341 gcgtcttctt ttggcaccag catcatcgcc acgccaatcg ccgttacgcc gccgatcaac
     5401 ttgccgacaa tcatcgggaa gatcatggcg ttcatgttgg cagcggcgaa gcctaagtgg
     5461 tcgcccaggg cgaaagcagc ggaaacggcg aaggcgcagt tgatgacttt gccgcgggta
     5521 tccatctgct tcatcatgcc gaacatcggg atgttgttgg caagcgttgc caccatgccg
     5581 gctgccgcga tgttgttcat attcagcact ttaccgacgc tcatcagcgg tttttcaaac
     5641 cagcgagtca gcagcagcac cattggatac gcccctaaca gcacgcagga gatagaaccg
     5701 ataacttcaa tggcgcgcat cacttcaccg ggtttatcgc caggggccat aaagatagga
     5761 tccagtccgg ggatcagttc ccagccaagg aggaatttca ctaccgcagc ggcaagaccg
     5821 agggtgatca atgcaacgag gaatttggcg aagatctgga agccgttgat cattttttcc
     5881 gggatgaatt tcagccccag cgccaccaga atcgcgacaa tgatcaccgg gatcatgttc
     5941 atcaggatca gggcgaaagt gaattccacc ggctgaccgt tgatctgcac accggagtac
     6001 atagcaacca gaccaccagc aatacagcca atcggaatgg tcacaatgcc cgccagcacg
     6061 ccgagcgcca gataacgacg gtcagaaggt tcgataatgc cgagcgccac cggaatggaa
     6121 aacacaatcg ttggccccat catcgaccca agaattaacc cagagtatag ccacgctgct
     6181 acgtcgccgc ccgccagctc tttggcgagg aagaaaccgc ccatatcgca cgccagcagc
     6241 gttccggcga acattgacgg gtttgcgccg agcatttcgt aaaccgggat aattaccggc
     6301 ccgagtacgt gagccagtac cggtgccagc gcggtcatac cgaccatcgc cagacccaga
     6361 gcgcccattg ccataaagcc ttcttcgaac tgaccgcctg atccttcgat acttttaccg
     6421 aacttaccga ggaaacgagc agaaccgccg aactgcgaca ggatcctgtc tacggcagct
     6481 atcagcataa agaacatcat gatgtacatg atgatttcgt taattcccat cgcctttact
     6541 ccctgttagt tgttatttat tggcggatgc ggcgtaaacg ccttatccgc cctacatgtg
     6601 caatcccgaa ggccggatca gacgcggcaa gcgtcgcatc cggcatttat tgcgccgctg
     6661 cgtacaggcc gacaatctgc tccaggctgg cggtacgcgg gttgctgcgc agacaaatat
     6721 cttcctgcgc ggcctgcgcc catgcaccgt aatgcgcaga tgtcgcgcca acatcgccca
     6781 gtcgtttacc aatcccaact tccgcaatca gctcacttac cgcgttaata gcgtcacgat
     6841 cgtcggattt tttagttcgc agtgcccgac caatctgact aaaccgttcg cgacaaacca
     6901 tccggttaaa ttccatcacc gttggcagca acatggcgtt cgccagaccg tgtggaatat
     6961 gcagcgccgc gcccggctga tgcgccatcg cgtggcacaa cccaagaccc gcactggaaa
     7021 acgccattcc cgccatacat gaagccagca acatgctctc gcgcgcggca aggtcgtgac
     7081 cgtagcccac cgctttcggc agcgatttgc caatcatcgc aatggcacca atcgccagac
     7141 tgtcggtaaa cggtgtagcg ttcagggcgc tgtatgcttc aatggcatgg gttaacgcat
     7201 caatgccggt catcgccgtg acatgcgacg gcacaccttc ggtcaatgcg gcgtcgagga
     7261 tcgccacatc cggcatcagc gaggcatggg ctaacacctg cttgcgcccg ctcaccgcgt
     7321 cgataatcac cgttacattg gtggtttcag agccggttcc ggcggtcgtt ggaatggcaa
     7381 tcagcggcaa gcgcggttgc agaacgctga tttctgacat ctctgccagc gtgctatccg
     7441 ggttcgtcac cagcaacgcc acggctttcg ccgcatccag caccgagccg ccgccaaatg
     7501 cgatcacccc atcacagcct gactcacgca actgcgccac ggctgcacac acgtcggtga
     7561 tgcacggttc gcccaccgga catggccaga gcgtcatggc gataccttta acggccaggc
     7621 tacgcgtcag cccggcggtc atccctgcct gatgcaaaaa gctgtctgcc atcacgaaca
     7681 gatgtttcag cccacgcgtt tgcgcttgct gtccgcaact gctcaccgag cccggaccgc
     7741 aaagcgtcac cggtggaacg ctaaatgttt ttacccgttg cagattcagg gtatcgaacg
     7801 cctgaaagag cgcggtctgc aattcatttt gcatatagcc cctccgcttt ctctcgccca
     7861 ctactggcga tcgccagcgg ggtcataaac aaactgtgct gcggtaaatg cacctgtaac
     7921 gccgggaatt gtttgcgaaa cagttccgcc acgcccggtt gcatacaaga gccgcccgcc
     7981 agccataaat cagtaatgcc ctgcccttca atatgccggg cgacaatgtc cgccattttt
     8041 tcgtacaccg gtttcaccgc gggccaaatc tcgtcaccat gaccgcgctt gtactgctct
     8101 gcctcttcca gcgaaatacg acgatttccg gcgagagtca gagagatgtg atgtccgccg
     8161 gtggcttcat ccgccgagta cgtcaccttg ccctttttca cgatggcaat gccggtagtg
     8221 ccgccgccga tatccactac accggcgttg tccagttgca gcagatccgc gactgcggtt
     8281 ggctcatcca gcacatggct cacttccagc ccggcagatt ccagcacgtt gatggaaata
     8341 cgcgggtccg tacctggagg aaatgaagtc gccgcatggc taaaacgaag accgaattgc
     8401 tgctcaagag tgtcgagatg gcgacgaaca atggtgaccg caccgaagaa atcccagacg
     8461 atgccgtcgc ggaccacatc ggcccagtcg aggcataccg ccaccggctg accgtcgcgg
     8521 tcaacaacca tcgacaccac atcgcaggtg cccagatcca cccccagcca cagcggagat
     8581 tccgtcgcgg cgggcgtctg gttacacagc gttgccgccg tttgcagacg tggggtgagc
     8641 cattgttcgt cgtgcgccat ctgttactcc ttaaacaatg cgaaacgcat cgactaatac
     8701 acagcgacgc agacggacaa acgtacgcgc gctggttacc ccttcaccgg ttggcgtggt
     8761 gatggtcatg gtggtccagc cttccccgcc cagccccagc ccggcaatgc acggtccgtt
     8821 cttaacgaaa atgctggtat caatagcatt cgccatctgg ttcatgtttt cgatgttgcg
     8881 cgagtgcatt gccgccgtgt ggtggcaacc gccttccagt ttcaccgcca gcgcaatggc
     8941 atccgccacg ttggcgacgc gcacgacggg cagcaccggc atcatcagtt cggtcacggc
     9001 aaacggatgt tctgcagtgg tttccacaaa cagcaaacgc gtttcttgcg gcacgttcag
     9061 gccgattgcc gccgcgattt tcgccgcatc gcgaccaacc cagtcacggc tgacggtgcc
     9121 ttttccgcgc tcgtcgatat ttttcagcaa caccggttgc agctgctgcg cctgttctgc
     9181 ggtcagtttc accgcgtgct ggccttccat cagacgcatc agttcatcgg ctacgctatc
     9241 aacaacaatc agtacctttt cgtcggcaca aatgatgttg ttatcgaaag acgcgccttt
     9301 gacgatggac tgagcagcac gggcgaggtc agcggtttca tccaccacta ccggcgggtt
     9361 gccagcgcct gcggcaatca gacgtttatt ggtgtgttta cgcgccgctt ctactaccgc
     9421 ttcgccgccg gttaccacca gcaggccgat acccggaaac ttgaacaagc gttgcgcggt
     9481 ttcgatatcc ggatttgcca cagtaaccag taagttttcc ggcccacctg cggcaacaat
     9541 cgcctggttg agcagcgtaa tcgcccgctg ggagactttt ttcgccgccg gatgcggggc
     9601 aaaaatgacg ctgttgcccg cggcaatcag gctgatggcg ttattaatta cggttgccgc
     9661 cgggttagtg gacggagtta ccgaagccac cacgccccag ggcgcgtttt caattaaagt
     9721 caggccgttg tcgccggtca gcacctgcgg agagagacac tcaacgcctg gcgtgccgcg
     9781 cgcctgagcg acgtttttgg caaatttatc ttcaacgcgg cccatgccgg tttcactgac
     9841 ggcaagttcc gctaaatctc tggcgtgttt ttcgcctgcc tcacgaatgg cagcaatggc
     9901 taactggcgc attgccacgc tttttaaccc ttgctgggcg actttggctg ccgcaacggc
     9961 gtcatccagg gacgcgaaaa cgcccatctc atgaacggcg gcggacggcg tgtcactgct
    10021 ttgcattttc agcagtaccg ctttcaccac ctgttcaata tcctgttgat tcatgatgtt
    10081 ctgccttatt tgtggaaaat tacctgaccg ccagacacca cctcatcgac aatgccaatc
    10141 acgcacagat cgaccggtga cgtttcgctt ttatgcgcct ggcgggcgga actgccactc
    10201 accagtaaca cccactcccc ggttcccgcg ccaatattgt cgatggcgac ggcgcattgc
    10261 ccgtcaggat taccttgtgg atcaatcatt tccaccatca gcaatttgtc atgcgccagt
    10321 ccgtgatggc gtacggtaca aacaatttgt ccagtgacga ctgccagttt catacccgcc
    10381 tccgtggcgt atttcaggta aaagctcccc ctaccctccg cagaaggtaa aatgaaaaag
    10441 gagagagcgt gacgcccgaa tcgacgtcac acagggtgat tacaggttgc tgctatcgcc
    10501 tttcaggccg atcgggaaga cttcttccag gtcaccgtgc ggacgcggga taacgtgtac
    10561 ggataccagc tcgccaatac gctgtgccgc tgcggcaccg gcatcggtcg ccgctttaca
    10621 ggctgcaaca tcaccgcgaa ccatggcagt acacaggccg ccgccaatct gcttaacacc
    10681 aaccagcttg acgcgcgcag ctttcaccat cgcgtcagaa gcctcaatca gtgcaaccag
    10741 gccccgggtt tcgatcattc ctaatgcttc cattgtgctt tcctctttat cagggtccag
    10801 aacgggaccg ttcattcaac cagtgtttgt aaactgcttt cgcggttcac ttttgtctga
    10861 cgcggcacag ctgccaccag cgccagctcg ataatttcct gcacgctaca accacgagag
    10921 agatcgtgca tcggcgcggc aagtccttgt atcaatggcc cgacggcacg atatccgccg
    10981 agtcgttgtg cgattttgta gccaatattt ccggcttcca gcgacggaaa aaccatcaca
    11041 ttggccttgc cctgtagcgg gctggcaggc gctttttgcg ccgccacttc cggcacgaag
    11101 gcggcgtcaa actgtaactc gccatcaacc accagctttg gtgcgcgctc acggacgatt
    11161 tctgtcgcct gctggacgtt ggcaacgcag gggtgacggg cgctaccgtt actggaaaac
    11221 gacagcatcg ccacgcgcgg ctcttctccg gtgatggcgc gccaggtttc ggcactggca
    11281 agcgcgatat ccgccagctg cgccgccgtc ggctgtggca ccacgctgca atcggcaaag
    11341 cccaacgccg ggccgctgta ctgtggcaac atcaggaaaa tggaagagag cgttttacag
    11401 cctggctgca agccgataat gcgtaatccg gcacgcagca catttgccgt ggaagagagg
    11461 ttgcccgcga tacagacatc cgctttaccg gcgctgacca ttgctgcggc gaacatcaga
    11521 gggtcggtaa gtttttccag cgcatccggc ggcgtttttt cgcccgcgcg ggccagccag
    11581 cgatgagcaa attcttcccg cattgcgagg ttgccatgcg gatcaatcac ctatagcccg
    11641 tccatcgcca cgccgtgact gagcgcaaac tgacgaagtt caaacggatt ggcgaccaga
    11701 atgggcgttg ccagaccttg ttgatgtaaa tattgcgcgg ctttcagcac acgttggtct
    11761 aacgcatccg gaaaaaccac tctggcgggc gctcgcaacg ccagttcacg acaacgttca
    11821 ataatcattg cgtttccccc agtcgttgtt ggatctgctt caccgttagc ggctgcttcg
    11881 ttacgctcag gatcatcatc acgtagaccg tgctcgaaag gcggttcagc gcctgcaaaa
    11941 tgtccgggcg taatacttca aagctgcggg tgataaagac ctgcgccgct acggtttcgg
    12001 tttcccgcac tttggtgcgt agcagattga gcaacgcagc atcgcgcccg tggctggctt
    12061 ccggcaccag atgatcgtgg tcgagatagc gcagcggctg atgagaaagc cgatgcagat
    12121 cttcgtcaga aagcccgaca atcgcctggc agcccagcgg ttcccccagc gcatcggcgc
    12181 gcataatgtt gccaagacgc gaacggatat ccgccagcca cggctgccac ggttccgcca
    12241 gttcaatttg cagccacact gccagcgcaa tggtgctgtc gagaaccgcg cgaaaaccca
    12301 gacgcggatc gcttttggcg accatttttt ccgccgacag gtgagtcagc gtatccggct
    12361 tttttgccac cggctggcgg cacagttcgc agcacgcctg cggatgttca tcgctactgg
    12421 tcagcccatg aacgggctgc ggctgctgct gttcatcgtc aacaaacagg cggccctgct
    12481 cgtcaataaa cttgatgcgc agatggcggc tttccagcaa ctcccgggca gagggcgtca
    12541 ggcgactgtc cgcagggaga tggatctctg cgccttcgct gagcgtatgg ttcgctctta
    12601 gccatgcttc ggtgatgaaa tctttcatag ggattgccag ttagccggcc aggcgacata
    12661 caggaatttc acgctggatg tcgtaccaaa ttcgatgctg gagcctttcg ggataaacat
    12721 cacgtcgcct gctttggcga tcatggtttc gccttcgtgg cgaacatgta gctccccttc
    12781 cagcaccatg tcgatttcgt cgtagttcag ggtccacggg aagaatgcgt tctcccactg
    12841 cataaaaccg gcggccatgc tgctgccgtc gtctccggtc acaagatcag tcaagcccac
    12901 gcaatgcggc tctgcgccgt cgaaacgacc aaacttgacg ctgctgccat caatcacttt
    12961 gatgccgcct ttgccggtca ccgatttgaa gctcggctgc attgcgccct gctccagcga
    13021 ctgtttttcc ttcatcactt tttccatcag ctgtgcgacc aggctttcgg taaactggcc
    13081 ttccggcagc tgggcaatga tggtttcgcg gatgcgctgg ctttcggttt tgtcggctgg
    13141 cacgctggcg ggcacggacg ccgttaccgg gatcgattca tcacattcgg tgatggtaaa
    13201 acccagtaga tccgccactt cacgggcttc cggggtaata atgctggcgc gcagaaccac
    13261 ggacattgcc tgttcgccgc gcgcgtgtgc ttcacgaata tcattcgctg tgatgagttt
    13321 tttcacctgc ttcctcctgt ttggtgagag atgccagata atccaccagt tgctgcacgc
    13381 tttgcgggtc gtggctgttt agctcgaaaa tcggctcttc gaaacccgtc tcaagcaata
    13441 actttcgcgt tgcggcgacg tcagcatctg gcatgtccgt tttactgatg acggcgattt
    13501 gccgtttgct gacgccaata tccagcaacc cggcaggcaa ccgactttcc gggtcatttg
    13561 cgccatggac atagatcagc atgtctacat cctgtagcgt ggtaattaag gcgtgatacc
    13621 agcggggatg gctaaaatat tcacccggcg tatcaatatc gcccttatca ttaaattcca
    13681 cggcctgtgt ttttctggcg agggtataat tcccctgcaa cgcattaaat aacgttgttt
    13741 ttcctgcccc gacgctaccc acaaacgcaa ttcgtttcat gaatgcctcg tattaacttt
    13801 tggtcatttc acaaagcgta taatttaata agcgacctaa accgctgacg gtttgtgata
    13861 acgcttcttc tactgcgccc accgagccat aaatcaccaa tgcaccgctg aagcggtcga
    13921 gaaaaccgat atgcacatca gcggctttca gcgctaaatc accggcgatc atcgccgttt
    13981 cgccgggagt tagcgtcata atgccaattg cgcccgcatc cggaacgccg attttcttcg
    14041 ccagttcctc accgggatgc gcgatgagat gcgccagtgt gacctgtttg ccgggcacaa
    14101 attcctgaat gatgcgttct ttatccatga gtcgccgcct gtgaaaaact ttcgtgtatc
    14161 gtgacaattt ttcgtcagag taaataacaa aattcggcaa gcgcgtttaa aaggtgaggt
    14221 agatcactaa gaatggagag atattcgctg tggtgcataa actttcatat gcaacgcatg
    14281 aatataaata aaataatatc gagatattat atagactgct ttttttatta ttcgcaaact
    14341 cagtaataag ctttactaat atattgcagc caaaccgctt accctgaata ttcagggtaa
    14401 gcgtgagagt taaaaaagaa ttacagcggt tgggtttgcg cttcaaccac ggccagcgcc
    14461 accatgttga cgatacgacg caccgatgcg atcggcgtta acacgtgaac cggtttcgcc
    14521 acgcccatca gcaccggacc gacagtcaca ccttccgagc tggaaacgcg cagtaagttg
    14581 taactaatgc gggcagcttc catgttcggc atcaccagaa tattggcaga acctttcaaa
    14641 gggctgtccg gcatacggtc gttgcgaatc gcttccacca gcgctgcatc gccgtgcatt
    14701 tcaccatcaa tcatcagatc tggcgcacga tccctgacca gttccagcgc ctgacgcatt
    14761 ttgctcgacg acgggcagtc agaagaacca aagttggagt gcgacaacag agcaacgcgc
    14821 ggctcaatac caaaacgacg gacagtttct gccgccatca aggtgatctc cgccagctct
    14881 tctgcgtctg gttcgtcatt gacgtaggta tcggcaataa aggtgttacc actcggcagc
    14941 agcagcgcgt tcattgcacc tgcggtgtga acgccatcgc gataaccaaa gacatttttc
    15001 accacgctaa aatgttcatg ataatcgccc accgtaccgc agatcattgc atcggcttcc
    15061 ccacgctgaa ccatgatcgc gccgatcact gtcgggttac tgatcagcgc ccgctgcgcc
    15121 tgttcctgag tgacaccgcg acgcttcatg atctggaagt attcggtcca gtactcttta
    15181 aagcgcggat cggattcgtt attgacgatc tcaaaatcaa cgcccgcttt gatctgcaag
    15241 cccagtttct gaatgcgcat ttcgatcacg ttcggacgac cgataaggat cggtttcgcc
    15301 agtcccagcg ttaccagttc ctgagtggca tgcagaacgc gcgcctcttc cccttctggc
    15361 agaacaacgc gcttcggcgc tttgcgagcc tgggagaaaa tcggcttcat aaacaggttg
    15421 gttttgtaaa cgaactcagt cagcttgtcg atgtagacgt cgaaatcagc aatcggacgc
    15481 gtcgccacgc ccgactccat cgcggcttta gcgaccgcag gagcgatctt aacgatcaag
    15541 cgcggatcaa acggttttgg aatgatgtat tccggaccaa agctcagatc ctgatcgcca
    15601 tacgctgaag ccaccacttc gctctgttcg gcgtgggcca gttccgcaat cgcgcgtacc
    15661 gccgccagtt tcatctcttc attgatagcc gttgcgccaa catccagcgc gccacggaag
    15721 ataaacggga agcaaaggac gttgttgacc tggtttggat agtcagaacg accggtgcaa
    15781 atgatggcat ccggacgcac ttctttcgcc agcggcggca gaatttccgg ttccgggttc
    15841 gccagcgcca ggatcattgg cgcacgagcc attttcttca ccatttcctg ggtcagcact
    15901 ttcgggccgg aacatccaag gaaaatatcc gcgccttcaa tcacatcatc gagggtacgt
    15961 ttgccgtcat ccaccaccgc atatgcggct ttggtttccg ccatgtttgg ctcacggccc
    16021 tgatagataa cgccttttga atcgcaaacc acgatgttat gtttttgcag acccagcgct
    16081 accagcaggt tcatacaggc gattgctgcg gcacccgcgc cggaaaccac catccgcacg
    16141 tcggagatgt ttttctccac cacgcgcaag ccgttgagga tggcggcagt gctgatgatt
    16201 gccgtgccgt gctgatcgtc gtggaatacc ggaatattca tccgctcgcg cagtttctgt
    16261 tcaatataga aacattctgg cgctttaatg tcttcgaggt tgattccgcc gaaggtcggt
    16321 tcgagcgcag cgacaacttc aataaatttg tccgggtcga gttcatcaac ttcaatgtca
    16381 aatacatcaa tcccggcgaa tttcttaaac agaacgccct tgccttccat caccggtttg
    16441 ccagccagcg cgccaatgtt gcctaacccc agcaccgccg taccgttaga gatcaccgcc
    16501 accaggttac cgcgcgcggt atatttgtag gcttttaacg ggtctttttc gatttcaaga
    16561 caaggtgcgg caacgcctgg tgagtaggcc agcgccagat cgcgctgcgt tgccagaggc
    16621 ttggttggag aaacctggat tttccctgga actggaaatt catggaaatc aagtgcactt
    16681 tgttttaact ggtcatccat ttggttgttc ctttcacgta acgttcacaa ataaagtgtg
    16741 tgggcaacag cccctgccca caacgtggcg cacattatta ccctgccgga gtctacagac
    16801 tttgagcaag tccaaactct caccattaat ataatgtttt ggtaataatc ctataacact
    16861 gatgttacct gcttaatcca gcaataccat gcctgtctgc tatgcttttt tgatgcgttt
    16921 agcgaaattt ctcagaagtg tgaattaacg cactcatcta acactttact tttcaaggag
    16981 tatttcctat gaacgagtta gacggcatca aacagttcac cactgtcgtg gcagacagcg
    17041 gcgatattga gtccattcgc cattatcata cccaggatgc caccaccaat ccttcgctgt
    17101 tactcaaagc tgccggatta tcacaatatg agcatttaat agacgatgct atcgcctggg
    17161 gtaaaaaaaa tggcaagacc caggaacaac aggtggtcgc agcgtgtgac aaactggcag
    17221 tcaatttcgg tgctgaaatt ctcaaaatcg tacccggtcg cgtgtcaaca gaagttgatg
    17281 cacgcctctc ttttgataaa gaaaagagta ttgagaaggc gcgccatctg gtggacttgt
    17341 atcagcaaca aggcgttgag aaatcacgca ttttgatcaa actggcttcg acctgggaag
    17401 gaattcgcgc ggccgaagag ctggaaaaag aaggtattaa ctgcaacctg acgctgctgt
    17461 tttcttttgc ccaggcgcgg gcctgtgcag aagcgggcgt ttttctgatt tcgccgtttg
    17521 tcgggcgtat ttatgactgg tatcaggcac gcaagccgat ggacccgtat gtggtggaag
    17581 aagatccggg cgttaaatcg gtgcgcaata tctacgacta ctataagcaa caccactatg
    17641 aaaccattgt gatgggcgcg agcttccgtc gcaccgaaca aatcctcgcc ttaaccggct
    17701 gcgatcgact gactatcgca ccgaatttgc tgaaggagct gcaggaaaaa gtttcgccag
    17761 tggtacgtaa attaatcccg ccttctcaga cgttcccacg cccagcaccc atgagcgaag
    17821 cggagttccg ttgggagcac aatcaggatg cgatggcggt agaaaaactg tctgaaggca
    17881 ttcgtctatt cgccgttgat caacgcaaac tggaagatct tcttgccgcc aaactataaa
    17941 ccagccacgg agtgttatat gtcccgaaaa gaccttgcca atgcgattcg cgcactcagt
    18001 atggatgcgg tacaaaaagc caattctggt catcccggcg cgccgatggg catggctgat
    18061 attgccgaag tgctgtggaa cgattttctt aaacataatc ctaccgaccc aacctggtat
    18121 gatcgcgacc gctttattct ttccaacggt cacgcgtcga tgctgctcta cagtttgcta
    18181 catctgaccg gttacgacct gccgctggaa gaactgaaga acttccgtca gttgcattcg
    18241 aaaaccccag gccacccgga gattggctat acgccaggcg ttgaaaccac caccggcccg
    18301 cttggacaag gtttggcgaa cgccgtcggg ctggcgatag cggaacgtac actggcggcg
    18361 cagtttaacc agccagacca tgagatcgtc gatcacttca cctatgtgtt tatgggcgac
    18421 ggctgcctga tggaaggtat ttcccacgaa gtctgttcgc tggcaggcac gctgggactg
    18481 ggcaagctga ttggttttta cgatcacaac ggtatttcca tcgacggtga aacagaaggc
    18541 tggtttaccg acgatacggc aaaacgtttt gaagcctatc actggcatgt gatccatgaa
    18601 atcgacggtc acgatccgca ggcggtgaag gaagcgatcc ttgaagcgca aagcgtgaaa
    18661 gataagccgt cgctgattat ctgccgtacg gtgattggct ttggttcgcc gaataaagca
    18721 ggtaaggaag aggcgcacgg cgcaccactg ggggaagaag aagtggcgct ggcacggcaa
    18781 aaactgggct ggcaccatcc gccatttgag atccctaaaa agatttatca cgcctgggat
    18841 gcccgtgaaa aaggcgaaaa agcgcagcag agctggaatg agaagtttgc cgcctataaa
    18901 aaggctcatc cgcaactggc agaagagttt acccgacgga tgagcggtgg tttaccgaag
    18961 gactgggaga aaacgactca gaaatatatc aatgagttac aggcaaatcc ggcgaaaatc
    19021 gctacccgta aggcttcgca aaatacgctt aacgcttacg ggccgatgct gcctgagttg
    19081 ctcggcggtt cggcggatct ggctcccagc aacctgacca tctggaaagg ttctgtttcg
    19141 ctgaaggaag atccagcggg caactacatt cactacgggg tgcgtgaatt tggcatgacc
    19201 gctatcgcca acggcatcgc gcaccacggc ggctttgtgc cgtataccgc gacgttcctg
    19261 atgtttgttg aatacgcccg taacgccgcg cggatggcgg cactgatgaa agcgcggcag
    19321 attatggttt atacccacga ctcaattggc ctgggcgaag atggtccgac gcaccaggct
    19381 gttgagcaac tggccagcct gcgcttaacg ccaaatttca gcacctggcg accgtgcgat
    19441 caggtggaag cggcggtggg ctggaagctg gcggttgagc gccacaacgg accgacggca
    19501 ctgatcctct caaggcagaa tctggcccag gtggaacgta cgccggatca ggttaaagag
    19561 attgctcgtg gcggttatgt gctgaaagac agcggcggta agccagatat tattctgatt
    19621 gccaccggtt cagagatgga aattaccctg caagcggcag agaaattagc aggagaaggt
    19681 cgcaatgtac gcgtagtttc cctgccctcg accgatattt tcgacgccca ggatgaggaa
    19741 tatcgggagt cggtgttgcc ttctaacgtt gcggctcgcg tggcggtgga agcaggtatt
    19801 gccgattact ggtacaagta tgttggtctg aaaggggcaa ttgtcgggat gacgggttac
    19861 ggggaatctg ctccggcgga taagctgttc ccgttctttg gctttaccgc cgagaatatt
    19921 gtggcaaaag cgcataaggt gctgggagtg aaaggtgcct gatggtgatc gccggatgct
    19981 gattgccgga tgcgacgctg acgcgtctta tccggcctac agcgtcttat ccggcctaca
    20041 tgtccccgca attttgttta acgggtgatc cacaacgtgg gccaggcgtc tggcccatgc
    20101 cagttatcgc aggtgggttc tgcggcataa cgcaccaggc gaaaacgctg accgtcaaag
    20161 cgccagcgcg cctgaatgcc acaatcgctt aatccgcgcc ctttcgctaa ggtcaccagt
    20221 tcacgcgatt tctcatcaaa tgttgcgttc atcagttcca gttcattcgt ctcctgaccg
    20281 ttgttgaacg gcaaacgcaa ccgaaccggg cgcgaggcta gtggcttttt acgcgacaca
    20341 atccatgcca aatcaatggt gttataggcc cctgcctcac agctaatcat catcagcgct
    20401 ttatcatcag tcagcgcagt gacattcacc tcacgacgta acggatcaag cgagcagcgc
    20461 agaccattca tccgccagtt tccataatcc agcaaatcgt tgcgttcttc gagtgagagt
    20521 ggcgtcggcg ttgggttaac caccgcgacc tctttcagcg caggcgcggg cggtacgctg
    20581 agcggcggtt cgtccccttt cttgatccac gcggtttcac tgccaacgcg cttttgctga
    20641 gcatcaataa acaacaacgc cgcttttaag ccactcagag aaatggtctg attgccgtcg
    20701 cgtaaggtga tcgccttccc ttcctgaatc atctgcaaaa acgcggtgat ggttgccgta
    20761 tcatcggtta ctaataacca tggtgaaatc cgccacttgt caccacttaa cgccaacggc
    20821 tcaccatcta acagcagccg tggcgctatc tctccttcag acgcatccgg cgacttcaac
    20881 ccgccgcgct caatacgtaa aacggcatcg gtatgcgccc cggcgctgcg gctcagggtc
    20941 atcaccagtc cattatgatc gcccgtattg cgcgccacgc agaagttttg gttattacag
    21001 gtgacctgcc agtcggaaaa cgcccgttgc gctggtgccg cccacaccaa agacgtcggc
    21061 aacaaagcga aaaaaaagag aaggaaaatg cgatagcgca tggacggtac gaccccagaa
    21121 gttcaaacaa acagcacaag tcgtatcttc gttgcgcagg tagcgcggct caatcagatt
    21181 tgtcggattg agccaataat aaacaacgaa atattattgt tatttttcag tccattaaat
    21241 gtgacgtttg caaatagtta agcaataaca ccgtcttacc gtcacgtatc tcgccggttt
    21301 tgatcatctc cagcgcctgg ctgaacggca gctcgagcac ttcaatatct tcatcttcaa
    21361 caccgccacc ggcgttagcg cgctgattgt cactgtattc ggcgataaaa aagtggatta
    21421 gctcagtcac accgccaggc gacatataca gttcaaataa tttgcgcact tcgcccactt
    21481 catagcccgt ctcttcaatc gcttctttac gaatgcacac ttccggttcg tcgttatcca
    21541 gcagcccggc gcaggtttca atcagctgcc cgctttcatt gccattaacc caggtagcga
    21601 cacggaactg acgaatcaga accacggtct ttttcttcgc gttgtacagg aggatcgtcg
    21661 caccattacc gcgatcgtaa acttcacgtt tatggcggat aacttcgcca tctttgcgcg
    21721 tgagatcgta agtaatgttg tgcagggtga aatagttatc ggagagaatt ttgtctttaa
    21781 tgagggtgat ttgttgcgtc atagcgactc cacagcgcga aatgaacaat tatcttacgc
    21841 tgtgaagtcg ggtttgtctg cgcaggctat cagtgagatt tgactgattt tacccccagc
    21901 cagtcaataa tgccctgtgc cgcatgacgt ccttctgcca ttgcggtaac caccagatcc
    21961 gcaccacgca cggcgtcgcc accggcgaag attttcggat tggtggtctg gtaacggtac
    22021 tggctttcca catccgcgat gatgcggccc catttgtcta ccgttacacc gtgcgactcc
    22081 agccacggca tcccgtgtgg attgaagcca aacgccataa tcaccgcatc ggctggcatg
    22141 acaaattcac tgccctcaat cggtacaggt cgccgacgcc cctgggcatc cggctcgcca
    22201 agacgcgtgc gcaggaaacg aattccgcaa acgtgacctt gttcattcag ctcaagcgcc
    22261 accggctgga cgttaaattc gaagttggcc ccctcttcgc gggcgttctt cacctctttc
    22321 ttcgagcctg gcatgttagc ttcatcacga cgataagcgc aggtgacgtt actcgcgccg
    22381 tggcgcagcg cagtacgcac acagtccatc gcggtgtcgc cgccgcccag taccacgacg
    22441 ttaagtccgg cggtattgat aaacggctct tccggtagct cttcgagccc catcacctgt
    22501 ttagtgttgg caatgaggaa cggcagcgcg tcataaacgc ccggcgcatc ttcattgggt
    22561 aaacccgctt tcatggaacg gtaagtgcct acgccaacga agaccgcgtc gtattgttcc
    22621 aaaagcgaat ccaaagagac atctttaccc acttcacaat tgagttcgaa gtgaatcccc
    22681 atcgcgctga agatttcccg acggcgtgcc agcagggatt tatccagttt gaaagaagga
    22741 atgccgaaag tgagcaagcc accaatttct ggatggcgat cgtacaccgt caccgccacg
    22801 ccattgcggg tcagaacatc cgcacaggcc agccctgccg ggcctgcccc gataatcgcc
    22861 acccgcttgt ccactttggt gacatgactt aagtcaggac gccaaccttt cgccaacgcc
    22921 tgatctgaaa tatagcgttc aatgttgccg atagttacag caccgtgctc atcgcgaata
    22981 gtacaggcac cttcacacaa acggtcttgc ggacaaacgc gtccggtaat ttccggcagg
    23041 gtgttggtct ggtgagaaag ctcgacggcg gcgtcgatgt ttccggcttt caccagttca
    23101 atccactgcg gaatatggtt atgcagcggg caggtccatt cacaaacgct gtgctcgccg
    23161 cacttaaggc agcgcgaggc ttcccgttgt gcctggtcgg cgcgaaatgg cagataaatt
    23221 tcatcaaaac cggttttgcg cgcttcaatc gccagtttat ccggctcgcc acgcgcgggc
    23281 gttgcctgca tttgttcgac tttactcatt accggcattt cttgcgccgc ggtactggca
    23341 tgccacggtt gatgttcctg acgcgcggtg cgcaagcggc gggatttcgc catgccggag
    23401 agtgcgacgt cagtgaccag ttgcagcgcg tccgccgggc aattctcaac acaggcagga
    23461 ccgttttcgc gccccgcaca aaggtcgcat ttatgcgccg tggcttttac ttttcctgcc
    23521 gcgacgggtg tcaggacgat ttgcatcgta ccaaaaggac aggccaccac gcaggattta
    23581 cagccaatac acttttgctg attgacctga atgctgtcat caacgtggct gattgcgcca
    23641 ttagggcagc tacgggcgca gggcgcatct tcacaatggt gacaggtcac tgcactacgt
    23701 tgctgttgat gtttgataac cgtaattcgg ggatgaaaat ggtgttggct caggacatgt
    23761 tgctcatcat tgtgagccat gacacaggcg atttcacaag catgacaacc cagacattgc
    23821 tgactgttgg ccataataaa acgattcata acgaccttct tttttggttg taaaaacctt
    23881 attctttata tgagtgttgt tattacccga cttacagggg aatcggcaat gttcatgtgc
    23941 ccagaataag taactatttc gctataaact gtggcagatc aaataatccc atcactgact
    24001 aaattgtgtt tcagctaact ggaacattaa tgattttttg tggagaagac gcgtgtgatt
    24061 gttaaacgac ccgtctcggc cagtctggcc cgggcctttt tttacattgt gctgctgtcg
    24121 attctttcca cgggtatcgc tctgctaact ctggcgagca gtttgcgcga cgctgaggct
    24181 atcaatattg ccggatcgct acgtatgcag agttaccgcc tgggctacga cttgcaaagt
    24241 ggcagtccac aactcaatgc acatcgccag ttgtttcagc aggcactgca ttcaccggta
    24301 ttaaccaatc tcaacgtctg gtatgtgcca gaagcagtaa aaactcgcta tgcgcatctg
    24361 aatgccaact ggctggagat gaataatcgg ctcagcaagg gcgatttgcc gtggtatcag
    24421 gccaatatta ataattatgt taatcagata gacctgttcg tgctggcttt acagcactac
    24481 gctgaacgca aaatgctgct ggtggtggcg atttccctgg ctggcggtat cggtattttc
    24541 acgctggtct tttttacttt gcgccgtata cgccatcagg tggttgcccc gctgaatcag
    24601 ctggttaccg ccagtcaacg tattgaacac gggcagttcg actcgccgcc gctggatacc
    24661 agtctgccga atgagcttgg tctgcttgca aaaaccttta accagatgtc gagcgagctg
    24721 cataaattgt acctttcgct ggaggcgtca gtagaagaaa agacccgcga tctccacgag
    24781 gccaagcgtc gtctggaggt gttgtatcaa tgttcccagg cgctgaacac cagccagatt
    24841 gatgtgcatt gtttccgcca tattttgcag attgttcgcg ataatgaagc ggctgaatat
    24901 ctggagttaa atgtcggtga aaactggcgg attagcgaag ggcaaccaaa cccggaattg
    24961 ccgatgcaga ttttaccggt gacaatgcaa gagacggttt acggcgaact gcactggcaa
    25021 aatagtcacg tttcatcatc agaaccgctg cttaacagcg tttcgtcgat gctgggacgc
    25081 ggtttgtact ttaatcaggc gcagaagcat tttcagcagt tattgttgat ggaagaacgt
    25141 gcaaccatcg cccgcgaatt gcacgactcg ctggctcagg tactttctta cttacgtatc
    25201 cagttgacgt tactgaagcg ttcgataccg gaagataacg ccaccgcaca aagtatcatg
    25261 gccgattttt cccaggcgtt gaatgatgct tatcggcagt tacgcgagct gttgactact
    25321 ttccgcctga cgctgcagca ggcggatctc ccctccgcgt tgagggaaat gctggatacg
    25381 ttacaaaatc aaaccagcgc caaactgacc ctcgactgcc gtctgccaac cctggcgctg
    25441 gatgcgcaaa tgcaggtgca tttgttgcaa attattcgcg aagcggtgct gaatgcgatg
    25501 aagcacgcca acgccagcga aatcgccgtc agttgcgtca ccgcgccgga cggcaatcac
    25561 acggtttata tccgtgataa cgggattggt atcggtgaac cgaaagaacc cgaaggtcat
    25621 tatggtctga atatcatgcg cgaacgcgcg gaacggctgg gtgggacgct gactttttca
    25681 caaccttccg gcggcggcac gttagtgagt attagctttc gctccgcgga gggtgaggaa
    25741 agtcagctga tgtaatgcct cctactgacc aaagaatact tgcacttaag gttcagtata
    25801 aaagggcatg ataatttaca ttaactcctt tttttctcca cgattggctc gtaccttgcc
    25861 gctacagtga agcaagtcaa gcctacaacg atacgcagaa acacgaggtc ctcttttaat
    25921 ggcgaatttc tttattgatc gccccatttt tgcctgggtg ctggcaatcc tgttgtgtct
    25981 gacaggtacc ctggcgattt tctcattgcc cgttgaacaa taccccgatc tcgcgccacc
    26041 gaatgtgcga gtgaccgcta actatcccgg cgcatcggcc cagacgctgg aaaacaccgt
    26101 gacccaggtt atcgagcaaa atatgaccgg cctcgataat ctcatgtata tgtcatctca
    26161 gagcagtggc accggtcagg catctgtcac tttaagtttt aaagcaggca ccgatccgga
    26221 cgaagccgtg cagcaagtac aaaaccagct gcaatcagcc atgcgaaagt taccgcaggc
    26281 ggtgcaaaat cagggcgtga cggtgcgtaa aaccggcgat accaatattc tgaccattgc
    26341 tttcgtctct accgatggtt cgatggataa acaggatatt gccgattatg ttgccagtaa
    26401 tattcaggac ccgttaagcc gcgtgaatgg cgtcggggat atcgatgcct atggttcgca
    26461 atattccatg cgtatctggc tggacccggc gaaactcaac agtttccaga tgacggctaa
    26521 agatgtcact gatgccattg agtcacagaa cgcgcagatt gcggttgggc aacttggtgg
    26581 tacaccttcc gtcgataagc aggcgctcaa cgccaccatt aacgcccagt cactgctgca
    26641 aacaccagaa cagttccgcg atatcacctt gcgggtgaat caagacggct cagaggtaag
    26701 gctgggcgat gtcgccaccg tcgaaatggg ggcggagaaa tacgattatc ttagccgctt
    26761 caatggtaag ccagcctccg ggctgggggt aaaactggcc tccggcgcaa acgaaatggc
    26821 gacagcggag ctggtgctca atcgtctcga cgagctggcg cagtacttcc cgcacggact
    26881 ggaatacaag gtggcgtatg aaaccacctc gtttgttaaa gcctccattg aagacgtggt
    26941 gaaaacgctg ctggaagcta tcgctctggt tttcctcgtt atgtatctgt tcctgcaaaa
    27001 cttccgcgcc acgctgatac ccactatcgc cgtgccggtg gtgttgatgg gaaccttctc
    27061 cgtactttac gccttcggtt acagcgtcaa caccttaacc atgttcgcga tggtgctggc
    27121 gatcggtctg ctggtggatg acgccatcgt ggtggtggaa aacgtcgaac gtattatgag
    27181 tgaggaagga ctcactcctc gcgaagccac acgtaaatcg atggggcaga tccagggggc
    27241 actggtcggg attgcgatgg ttctttcggc ggtatttgta ccaatggcct tcttcggcgg
    27301 caccaccggt gccatctatc gccagttctc tattaccatt gttgcggcga tggtgctgtc
    27361 agtactggta gcgatgatcc tcactccggc tctgtgtgcc acactactta agccactgaa
    27421 aaaaggtgag catcacgggc aaaaaggctt ttttgcctgg tttaaccaga tgtttaaccg
    27481 caacgccgaa cgctacgaaa aaggggtggc gaaaattctc caccgtagcc tgcgctggat
    27541 tgtgatttat gtcctgctgc ttggcggcat ggtgttcctg ttcctgcgtt tgccgacgtc
    27601 gttcttaccg ctggaagacc gtggcatgtt tactacctcg gtacaattgc ccagcggttc
    27661 aacccaacaa cagaccctga aagtcgttga gcaaatcgag aaatattact tcacccatga
    27721 aaaagacaac atcatgtcgg tgtttgccac tgttggttct ggccccgggg gtaacgggca
    27781 aaacgtggcg cgaatgttta tccgcctgaa agactggagc gaacgcgaca gtaagaccgg
    27841 cacctcgttt gccattatcg agcgtgcaac aaaggcgttt aacaagatta aagaagctcg
    27901 cgttatcgcc agcagcccgc cagcaattag cggtcttggt agttctgcgg gttttgatat
    27961 ggagttgcag gaccacgctg gagcaggtca cgatgcgctg atggcagcac gtaaccagtt
    28021 gctggcgctg gcggcggaaa acccggagct aacccgtgtg cgccataacg gcctcgacga
    28081 cagtccgcag ttgcagattg atatcgacca gcgtaaagct caggcgctgg gcgttgatat
    28141 cgacgatatt aacgacacac tgcaaaccgc ctggggttcg agctatgtta atgactttat
    28201 ggatcgcggt cgcgtgaaga aagtctatgt gcaggcagct gcgccgtatc gcatgctgcc
    28261 agatgacatc aatctctggt atgtccgaaa taaagatggc ggcatggtgc ccttctctgc
    28321 tttcgcgacc tcacgctggg agacaggctc gccgcgtctg gaacgctata acggttattc
    28381 tgcggttgag attgttgggg aagccgcacc gggggtcagt accggtacgg cgatggatat
    28441 tatggaatcg ttagtgaagc agttgccaaa cggctttggt ctggagtgga cggcgatgtc
    28501 gtatcaggag cggctttccg gcgcgcaggc tccggcgctg tacgctattt ccttgctggt
    28561 ggtattcctg tgtctggcgg cgttgtatga aagctggtcg gtgccgttct cggtaatgct
    28621 ggtcgtgccg ctgggggtta tcggcgcgct gctggcaacc tggatgcgcg ggctggaaaa
    28681 cgacgtttac ttccaggtgg gcctgttaac ggtcattggt ttatcggcga aaaacgccat
    28741 cctgatcgtc gagtttgcta acgagatgaa ccaaaaaggc cacgacctgt ttgaagcgac
    28801 gctccacgcc tgccgtcagc gtttacgccc gattctgatg acctcgctgg catttatctt
    28861 cggcgtattg ccaatggcaa ccagcacggg tgccggttcc ggtggtcagc atgcggtggg
    28921 tactggcgta atgggcggga tgatttcggc cactattctg gctatttact tcgtgccgct
    28981 gttctttgta ctggtgcgcc gccgcttccc gctgaagccg cgcccggaat aagcaataaa
    29041 aaaggcgaca tgctaatgtg tcgccttttt caactttccg attaagaacc tgctcagcgg
    29101 gttcttgctg tttgtacttt gtctcaggaa ttacttacga agcataactt cgataaagtc
    29161 tttccagttc cccagttcac gctcaatcat aacaacctct cttataatta tgggtattct
    29221 acggaaacaa tataccgtgg tgaagctaat ttactcgatt gctgcgatga ctacctccgg
    29281 gggacaaacc ttatgtaaat actatggtcc tgcggtgata atttgtatgt gatacacagc
    29341 aacatttcga gatattcata cggcatctaa tactgattta attctggtta aaatacagac
    29401 agataacaag atgaatattc ttaatgttta cgttaaaaat gtttaatatt atttaatagt
    29461 tgttaatttg aatacttcga taatgttata tttcctgata atcatttgca ggcaaaatgt
    29521 tttcaccctt aaatgagtat ttattctcat agatcgaaaa aggattcatt atggttacac
    29581 tttacggcat caaaaattgt gacaccatta aaaaggctcg ccgttggctg gaagccaata
    29641 acatcgacta tcgttttcat gattaccgcg tcgatgggct ggacagcgaa ttattgaacg
    29701 gttttatcaa cgaattaggc tgggaagcgt tactgaacac ccgtggtaca acctggcgta
    29761 aactggacga aaccacccgc aataaaatca ccgatgcggc ctctgcggcg gcattaatga
    29821 ctgaaatgcc tgcaattatc aaacgtccat tgctctgcgc gcctggtaag cctatgctgc
    29881 tgggtttcag tgattccagt tatcagcaat ttttccatga ggtgtagtct atgtcgtgcc
    29941 cggttattga gctgacacaa cagcttattc gccgcccttc cctgagtcct gatgacgcag
    30001 gatgccaggc tttgttgatt gaacgtttgc aggcgatcgg ttttaccgtt gaacgcatgg
    30061 attttgccga tacgcagaat ttttgggcat ggcgtgggca aggtgaaacg ttggcctttg
    30121 ccgggcatac cgacgtggtg ccgcctggcg acgccgatcg ttggatcaat ccgccgtttg
    30181 aacccaccat tcgtgatggc atgttattcg gacgcggtgc ggcagatatg aaaggctcgc
    30241 tggcggcgat ggtggtagcg gcagaacgct ttgtcgcaca acatcccaac catacggggc
    30301 gactggcatt tctgatcacc tctgatgaag aagccagtgc ccacaacggt acggtaaaag
    30361 tcgtcgaagc gttgatggca cgtaatgagc gtctcgatta ctgcctggtc ggcgaaccgt
    30421 cgagtatcga agtggttggt gatgtggtga aaaatggtcg tcgcggatcg ttaacctgca
    30481 accttaccat tcatggcgtt caggggcatg tggcctaccc gcatctggct gacaatccag
    30541 tacatcgcgc agcacctttc cttaatgaat tagtggctat tgagtgggat cagggcaatg
    30601 aatttttccc ggcgaccagt atgcagattg ctaatattca ggcgggaacg ggcagtaaca
    30661 acgttattcc gggtgaactg tttgtgcagt ttaacttccg cttcagcacc gaactgactg
    30721 atgagatgat caaagcgcag gtgcttgccc tgcttgaaaa acatcaactg cgctatacgg
    30781 tggattggtg gctttccggg cagccatttt tgaccgcgcg cggtaaactg gtggatgcgg
    30841 tcgttaacgc ggttgagcac tataatgaaa ttaaaccgca gctactgacc acaggcggaa
    30901 cgtccgacgg gcgctttatt gctcgcatgg gggcgcaggt ggtggaactc gggccggtca
    30961 atgccactat tcataaaatt aatgaatgtg tgaacgctgc cgacctgcag ctacttgccc
    31021 gtatgtatca acgtatcatg gaacagctcg tcgcctgatg agtggttctg caagaggaaa
    31081 taagcatgga ctggctggct aaatattggt ggattctggt gattgtcttt ttggtaggcg
    31141 tcctgctgaa cgtgattaaa gatctcaagc gcgtggacca taagaaattt ctcgccaaca
    31201 agccggagct tcccccgcat cgtgatttca acgataagtg ggacgatgac gacgactggc
    31261 cgaaaaagga tcaaccgaag aagtaagctg agtgctcaac cgtaggccgg ataaggcgtt
    31321 aacgccgcat ccggcaatgg tgaacgatgc ctgatgcgac gctcacgcgt cttatcaggc
    31381 caggtaatct gtcttttaca tcatttcaat cacatcatca tcgccaggct taccgccact
    31441 tagcgcttcg tcgaaataat gtttcggaat ggtataacgc aagtgatcca gggcaaattg
    31501 catactgcga ttgtcgatcg catggcctaa atcctccacg atatccagcg tcacgtcacc
    31561 accggcactg attaacgctt cctgcgccgc aaccgcatgc gccagatcga taaccggatc
    31621 ttcaccgcca tgaatcaggt gaattgtcgt ggctgtagac gcagtttccg gcaggctcgc
    31681 atagcgtccg ttaaaagcaa tgacgcgtga agcaaggccc ggttcggctt taatgctctc
    31741 cagcaccata atcgcgcctt gagagaaacc gatgagcgcc gtggcgttag cccctactcc
    31801 gctctgtttc tgccagtagc gcaccgtttc aataaacgtc ggcatgatcg catccacacg
    31861 cgcctggcga ttatcttccg taataccctg caccgaaaac cactgacgcc ccgccggatt
    31921 accgctcggc tccgcgccac cgacactcac caccagcgca tcaggaaaca gcggtgcaaa
    31981 ccagctacct atttccccca ttgccaccgg gttatccccg acaccgtgga aaagcagtaa
    32041 caactgttgc gcaggtttat ccgggctttg aacaacaaaa tggtcatgtt tcatggcgaa
    32101 ctccttaact gatgtcatta attttacgcc gctgactcac aatgaccatg ggagaaa
//
LOCUS       DAMZET010000033        31861 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.033, whole genome shotgun sequence.
ACCESSION   DAMZET010000033 DAMZET010000000
VERSION     DAMZET010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.033"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            124..200
                     /locus_tag="POB34_001663"
     tRNA            124..200
                     /locus_tag="POB34_001663"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:158..160,aa:Arg,seq:acg)
     gene            481..1047
                     /gene="yqaB"
                     /locus_tag="POB34_001664"
     CDS             481..1047
                     /gene="yqaB"
                     /locus_tag="POB34_001664"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HDI5918634.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            1044..1472
                     /locus_tag="POB34_001665"
     CDS             1044..1472
                     /locus_tag="POB34_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDI5918635.1"
                     /translation="MSEALSLLSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1545..3101
                     /gene="gshA"
                     /locus_tag="POB34_001666"
     CDS             1545..3101
                     /gene="gshA"
                     /locus_tag="POB34_001666"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDI5918636.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            3251..3766
                     /gene="luxS"
                     /locus_tag="POB34_001667"
     CDS             3251..3766
                     /gene="luxS"
                     /locus_tag="POB34_001667"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDI5918637.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGKGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3830..5368)
                     /gene="emrB"
                     /locus_tag="POB34_001668"
     CDS             complement(3830..5368)
                     /gene="emrB"
                     /locus_tag="POB34_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HDI5918638.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(5385..6557)
                     /gene="emrA"
                     /locus_tag="POB34_001669"
     CDS             complement(5385..6557)
                     /gene="emrA"
                     /locus_tag="POB34_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HDI5918639.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(6684..7214)
                     /gene="emrR"
                     /locus_tag="POB34_001670"
     CDS             complement(6684..7214)
                     /gene="emrR"
                     /locus_tag="POB34_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HDI5918640.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(7305..7640)
                     /gene="ygaH"
                     /locus_tag="POB34_001671"
     CDS             complement(7305..7640)
                     /gene="ygaH"
                     /locus_tag="POB34_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HDI5918641.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALTYG
                     LVWKVMAII"
     gene            complement(7630..8367)
                     /gene="ygaZ"
                     /locus_tag="POB34_001672"
     CDS             complement(7630..8367)
                     /gene="ygaZ"
                     /locus_tag="POB34_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HDI5918642.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(8491..9675)
                     /locus_tag="POB34_001673"
     CDS             complement(8491..9675)
                     /locus_tag="POB34_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5918643.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(9967..10959)
                     /gene="proX"
                     /locus_tag="POB34_001674"
     CDS             complement(9967..10959)
                     /gene="proX"
                     /locus_tag="POB34_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HDI5918644.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTHTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(11017..12081)
                     /gene="proW"
                     /locus_tag="POB34_001675"
     CDS             complement(11017..12081)
                     /gene="proW"
                     /locus_tag="POB34_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HDI5918645.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(12074..13276)
                     /gene="proV"
                     /locus_tag="POB34_001676"
     CDS             complement(12074..13276)
                     /gene="proV"
                     /locus_tag="POB34_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HDI5918646.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(13631..14590)
                     /gene="nrdF"
                     /locus_tag="POB34_001677"
     CDS             complement(13631..14590)
                     /gene="nrdF"
                     /locus_tag="POB34_001677"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDI5918647.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQLEEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(14600..16744)
                     /gene="nrdE"
                     /locus_tag="POB34_001678"
     CDS             complement(14600..16744)
                     /gene="nrdE"
                     /locus_tag="POB34_001678"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDI5918648.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYKIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(16717..17127)
                     /gene="nrdI"
                     /locus_tag="POB34_001679"
     CDS             complement(16717..17127)
                     /gene="nrdI"
                     /locus_tag="POB34_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDI5918649.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(17124..17369)
                     /gene="nrdH"
                     /locus_tag="POB34_001680"
     CDS             complement(17124..17369)
                     /gene="nrdH"
                     /locus_tag="POB34_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDI5918650.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(17617..17946)
                     /locus_tag="POB34_001681"
     CDS             complement(17617..17946)
                     /locus_tag="POB34_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDI5918651.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            18098..18442
                     /locus_tag="POB34_001682"
     CDS             18098..18442
                     /locus_tag="POB34_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HDI5918652.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(18479..18928)
                     /gene="alaE"
                     /locus_tag="POB34_001683"
     CDS             complement(18479..18928)
                     /gene="alaE"
                     /locus_tag="POB34_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HDI5918653.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            19596..20000
                     /gene="stpA"
                     /locus_tag="POB34_001684"
     CDS             19596..20000
                     /gene="stpA"
                     /locus_tag="POB34_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HDI5918654.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(20047..20571)
                     /gene="ygaP"
                     /locus_tag="POB34_001685"
     CDS             complement(20047..20571)
                     /gene="ygaP"
                     /locus_tag="POB34_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HDI5918655.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRHEQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPFPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(20581..20880)
                     /locus_tag="POB34_001686"
     CDS             complement(20581..20880)
                     /locus_tag="POB34_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI5918656.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            21063..21221
                     /locus_tag="POB34_001687"
     CDS             21063..21221
                     /locus_tag="POB34_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HDI5918657.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            21305..21754
                     /gene="kbp"
                     /locus_tag="POB34_001688"
     CDS             21305..21754
                     /gene="kbp"
                     /locus_tag="POB34_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HDI5918658.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(21755..22417)
                     /gene="csiR"
                     /locus_tag="POB34_001689"
     CDS             complement(21755..22417)
                     /gene="csiR"
                     /locus_tag="POB34_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HDI5918659.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(22438..23838)
                     /gene="gabP"
                     /locus_tag="POB34_001690"
     CDS             complement(22438..23838)
                     /gene="gabP"
                     /locus_tag="POB34_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HDI5918660.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            complement(24076..25356)
                     /gene="gabT"
                     /locus_tag="POB34_001691"
     CDS             complement(24076..25356)
                     /gene="gabT"
                     /locus_tag="POB34_001691"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HDI5918661.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGNFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKQ"
     gene            complement(25370..26818)
                     /gene="gabD"
                     /locus_tag="POB34_001692"
     CDS             complement(25370..26818)
                     /gene="gabD"
                     /locus_tag="POB34_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDI5918662.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALSSKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLENGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            complement(26841..28109)
                     /gene="lhgO"
                     /locus_tag="POB34_001693"
     CDS             complement(26841..28109)
                     /gene="lhgO"
                     /locus_tag="POB34_001693"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HDI5918663.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNAMELREREPNITGLGGIFVPSSGIVSYRNVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            complement(28129..29106)
                     /gene="csiD"
                     /locus_tag="POB34_001694"
     CDS             complement(28129..29106)
                     /gene="csiD"
                     /locus_tag="POB34_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HDI5918664.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            complement(29442..31694)
                     /locus_tag="POB34_001695"
                     /pseudo
     CDS             complement(29442..31694)
                     /locus_tag="POB34_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7995 a         8039 c         8027 g         7800 t
ORIGIN      
        1 ttgctttagc aacggcccga agggcgagcc gcaaggcgag taatcctccc ggatgcacca
       61 tctcttactt gatatggctt tagtagcgat atcaatatca gcagtaaaat aaattttccc
      121 gatgcatccg tagctcagct ggatagagta ctcggctacg aaccgagcgg tcggaggttc
      181 gaatcctccc ggatgcacca tattctccgt aaccttcagc aatgaaggta tcgaagagat
      241 agcggtatta accgcaggca ccagggagga taacgttgcg tcagcaacgg cccgaagggc
      301 gaacgccagt gagtaatcct cccggatgca ccatcttctc ccaaaaattt ccttcaaaaa
      361 tatctcattt ctaaataact actttatcca tttcctgaat tttcttctcc ttcgccatca
      421 gcgacaaaat caagcaatta cgctaaagta gcgtcatatt tttcgacttg cgagaagacc
      481 atgtacgagc gttatgcagg tttaattttt gatatggatg gcacaatcct ggatacggag
      541 cctacgcacc gtaaagcgtg gcgcgaagta ttagggcact acggtcttca gtacgatgtt
      601 caggcaatga ttgcgcttaa tggatcgccc acctggcgta ttgctcaggc aattattgag
      661 ctgaatcagg ccgatctcga cccgcatgcg ttagcgcgtg aaaaaacaga agcagtaaga
      721 agtatgctgc tggatagtgt cgagccgctt cctcttgttg aagtggtgaa aagttggcat
      781 ggtcgtcgcc caatggctgt aggaacgggg agtgaaagcg ccatcgctga ggcattgctg
      841 gcgcacctgg gattacgccg ttattttgac gccgtcgtcg ctgctgatca cgtcaaacac
      901 cataaacccg cgccagacac atttttgttg tgcgcgcagc gtatgggcgt gcaaccgacg
      961 cagtgtgtgg tctttgaaga tgccgatttc ggtattcagg cggcccgtgc agcaggcatg
     1021 gacgccgtgg atgttcgctt gctgtgagtg aagcgttatc gcttttatcg ttgtttgcca
     1081 gtagttttct cagcgctaca ctattacccg gcaactccga agtcgtgctg gtggcaatgt
     1141 tgctttccgg gatcagttat ccctgggttt tagttttaac agcaacaatg ggtaatagcc
     1201 ttggagggtt aactaacgtt atccttgggc gtttctttcc attgcgtaaa acatcgcgct
     1261 ggcaagagaa agctaccggc tggctgaaac gctatggtgc agtcacgcta ttattaagct
     1321 ggatgcccgt ggttggcgat ttactgtgtc tgttagcggg atggatgcgc atctcctggg
     1381 ggccagtaat cttttttttg tgccttggta aagcgttacg ctatgttgca gttgcagcag
     1441 cgaccgttca gggcatgatg tggtggcact aattgtaggc ctgcacatat ggtcaccatt
     1501 acagttatgc taattaaaac gattttgaca ggcgggaggt caatttgatc ccggacgtat
     1561 cacaggcgct ggcctggctg gaaaaacatc ctcaggcgtt aaaggggata cagcgtgggc
     1621 tggagcgcga aactttgcgt gttaatgctg atggcacact ggcaacaaca ggtcatcctg
     1681 aagcattagg ttccgcactg acgcacaaat ggattactac cgattttgcg gaagcattgc
     1741 tggaattcat tacaccagtg gatggtgata ttgaacatat gctgaccttt atgcgcgatc
     1801 tgcatcgtta tacggcgcgc aatatgggtg atgagcggat gtggccgtta agtatgccat
     1861 gctacatcgc agaaggtcag gacatcgaac tggcacagta cggcacttct aacaccggac
     1921 gctttaaaac gctgtatcgt gaagggctga aaaatcgcta cggcgcgctg atgcaaacca
     1981 tttccggcgt gcactacaat ttctctttgc caatggcatt ctggcaagcg aagtgcggtg
     2041 atatctcggg cgctgatgcc aaagagaaaa tttctgcggg ctatttccgc gttatccgca
     2101 attactatcg tttcggttgg gtcattcctt atctgtttgg tgcatctccg gcgatttgtt
     2161 cttctttcct gcaaggaaaa ccaacgtcgc tgccgtttga gaaaaccgag tgcggtatgt
     2221 attacctgcc gtatgcgacc tctcttcgtt tgagcgatct cggctatacc aataaatcgc
     2281 aaagcaatct tggtattacc ttcaacgatc tttacgagta cgtagcgggc cttaaacagg
     2341 caatcaaaac gccatcggaa gagtacgcga agattggtat tgagaaagac ggtaagaggc
     2401 tgcaaatcaa tagcaacgtg ctgcagattg aaaacgaact gtatgcgccg attcgtccaa
     2461 aacgcgttac ccgcagcggc gagtcacctt ctgatgcgct gttacgtggc ggcattgaat
     2521 atattgaagt gcgttcgctg gacatcaacc cgttctcgcc gattggtgta gatgaacagc
     2581 aggtgcgatt cctcgacctg tttatggtct ggtgtgcgct ggctgatgca ccggaaatga
     2641 gcagtagcga acttgcctgt acgcgcgtta actggaaccg ggtgatcctt gaaggtcgca
     2701 aaccgggcct gacgctgggt atcggctgcg aaaccgcgca gttcccgtta ctgcaggtgg
     2761 gtaaagatct gttccgcgat ctgaaacgcg tcgcgcaaac gctggatagc attaacggcg
     2821 gcgaagcgta tcagaaagtg tgtgatgaac tggttgcctg cttcgataat cccgatctga
     2881 ctttctctgc ccgtatctta aggtctatga ttgatactgg tattggcgga acaggcaaag
     2941 cgtttgcaga agcctaccgt aatctgctgc gtgaagagcc gctggaaatt ctgcgcgaag
     3001 aggattttgt agccgagcgc gaggcatctg aacgccgtca gcaggaaatg gaaaccgctg
     3061 ataccgaacc gtttgcggtg tggctggaaa aacacgcctg acagaaaaga aaaaggccac
     3121 tcgtgagtgg ccaaaatttc atctctgaat tcagggatga tgataacaaa tgcgcgtctt
     3181 tcatatactc agactcgcct gggaagaaag agttcagaaa attttaaaaa aaattaccgg
     3241 aggtggctaa atgccgttgt tagatagctt cacagtcgat catacccgga tggaagcgcc
     3301 tgcagttcgg gtggcgaaaa caatgaacac cccgcatggc gacgcaatca ccgtgttcga
     3361 tctgcgcttc tgcgtgccga acaaagaagt gatgccagaa agagggatcc ataccctgga
     3421 gcacctgttt gctggtttta tgcgtaacca tcttaacggt aagggtgtag agattatcga
     3481 tatctcgcca atgggctgcc gcaccggttt ttatatgagt ctgattggta cgccagatga
     3541 gcagcgtgtt gctgatgcct ggaaagcggc aatggaagac gtgctgaaag tgcaggatca
     3601 gaatcagatc ccggaactga atgtctacca gtgtggcact taccagatgc actcgttgca
     3661 ggaagcgcag gatattgcgc gtagcattct ggaacgtgac gtacgcatca acagcaacga
     3721 agaactggca ctgccgaaag agaagttgca ggaactgcac atctagtcag taaactatct
     3781 tcacaattaa ttgaaaaaag ccagttcaaa tgaactggct tagttgtact tagtgcgcac
     3841 cgcctccgcc gccacctgcg ccaaatggcg gtttagcaaa ccacaccagc cccagcagta
     3901 cgaggaatat cccggctgac atccagaaga tctcattggc ggaaataatc agcccctgat
     3961 tggtgatctg ctgggcaatc cagcctgatg cctgctgttg cgtcatccca agcccttcca
     4021 gttgactgta catcgcctgg gcattcgggt tgaacgggtt taccgactca gtcaactgcg
     4081 catggtgcat cgactcgcgg ttggtccaca tggtcgtggt tatcgacgtg ccgatagacc
     4141 ccgccagcgt tcgcgtaaag ttagagaggc tcgatgccgc cgccagtcgt tccggtggca
     4201 aaccagacag cgtaatggtg gtcagcggca taaagaagca ggccaccgca aacccctgga
     4261 taaactgcgg ccaggccgac gcgccaaaat ccatacctgg ttcaaaggta taggcacgcc
     4321 agtagaagca gacggcatac ataataaagc tgaaggttac cagccgccgc atatccagtt
     4381 tatgcgcgaa gcggccgata atcggcgaca ggatcaccgg aataatccct accggcgcag
     4441 aggccaaacc tgcccaggtc gccgtgtaac cgtagacctc ctgcaacaac tgcggcagca
     4501 gaacaatagc gccgaagtag agcatatacg cgaggctgat acacaagcag ccgatggtga
     4561 agttgcgcga cttaaacaac gacagatcga ctatcgggtt atcgtcggtc agctcccaga
     4621 caatcaggaa gcagatagcc accaccgcca ccacggtaag aatgataatt tcctgtgatg
     4681 aaaaccagtc cagctcttta ccgcggtcga gcataatctg caggctgccg ataccaataa
     4741 ccagcagtgc cagccccacg gcatcaatcc gccgccgttc ggtgcgggtt tcacgtccgc
     4801 gcagagtttg cagtgtcatc aacaccaccg ccacgccaat cggcacgttg atgaagaata
     4861 tccagcccca gtggtaatta tcgctgatat aaccgccgag gatcgggccg caaattggcg
     4921 cgacaatcac cgtcatcgac cacaacgcca gcgcgatcga gcgtttggct ggcgggtagt
     4981 tattcagcaa tagactttgc gaaagcggga tcaacggccc ggcgacaatc ccctgaatca
     5041 cgcggaagaa gatcagcata ttcaggctgc tggagacacc acacgcccac gacgcaatag
     5101 caaaggcgat ggtggaccaa aggaacagtt tcacttcccc gacgcgcttt gccagccagc
     5161 cggtaagcgg gatcgagatg gcattcgcca ccccgaaaga agtgattacc cacgttccct
     5221 ggctgagcga tgagcccaga ttcccggcga tagtggggat cgccacgtta gcaatggtgg
     5281 agtccagcac ctgcatgaat gtcgccagtg acagcgcaat cgtcataatg accagttgcg
     5341 cgccttccag cggtttttgc tgttgcatca cacgcacctc tggattagcc agcgttagct
     5401 tttacgatat cgtcgatcag tttattgaca ggtgccaggc tgatttcacg cgcggtactt
     5461 accgcgaccg gtgttgaacg tactttattt gccagtacct gaccgtcacg gttagtggta
     5521 ttgacgctca ccagcgtgga caaaccaatg cgcagcgggt attgctccag ctgtttctgg
     5581 tccagttcga tacgcacagg tagacgctga acgactttga tccagttacc ggtcgcattt
     5641 tgcgccggaa gcagtgagaa cgcgctacct gtgcccatat ccagaccaac cactttaccg
     5701 gtgtatttca catcatcgcc gtaaatatcg gtggtgatgg tgaccggctg accgatgcgc
     5761 atattggcaa tctgcgtctc tttaaagttg gcatccaccc acatattggt ggctggaacg
     5821 accgccatca gcggcgtcgt tgggctaatt tgcgcacccg gttgcacggc acgacgggaa
     5881 acataaccag tcatcggact gacaatacgg gtacgctcca gcgccagcca ggcgttacgt
     5941 acttcggtgg cagcctgttg cacggcaggc tgatcttcca gtttagtccc cagaatcatc
     6001 gcctgattgg cattgtattg ttgaatagcg acgtccagtt gcgcctgggc actggtgacg
     6061 gcgtcgcggg cgtgttgcag ctcttcgcga ccaatcaggt tggcattgcc cagcggcaca
     6121 cggcggttgt agtcgctttg tgctttcgcg agggcgattt tctgcacctc aatattcgcc
     6181 tgcaactgct tgctgttaat catcagctgg tgggtttggc gaacgctgga agccagtgca
     6241 gttttggctt tttcaaacgc ctggcgagca tctgtcgggt cgagagtgac cagaacatcg
     6301 ccttctttta caaaatcggt gttatcggcc cagactttcg tcacgctgcc agacacctga
     6361 gacataattt gaatctgatt ccctgccacg tatgcgtcat cggtttcttc gaaatgacgc
     6421 agtaccaaaa accaataaat ccctatcgct acggcaataa ttataaagag caaggtgaga
     6481 aggaggagca gacgcttacg tttgccgctc ttctttaccg gttgctgcgg ggtttgagtc
     6541 tccgcatttg cgctcatatt gttctccacg atcttcttaa ctcatcggct gagccgacct
     6601 gcttgccaaa aaggccagca tgttgcgatg ctggccagtc attttttctt ttataaatct
     6661 ggatttttga gcgagatgac gcgttagctc atcgcttcga gaaccacacc gtcttgttcc
     6721 atctggtcga gacgggagag caatttgcgg gtgatttgct cgagctgatc tttttctgtt
     6781 gtgctgagcg cggaccagag ttgatgcagg cagttatgct gcggtggtaa aacctcgcgc
     6841 aaaaactcgt gacctttttc cgttaattgc agatgcaggc agcggcgatc gttatcgctt
     6901 tcacgacgtt cgatccaacc gcgtttttcc agttcatcgg caatacgcgt cgcgttggta
     6961 cgggatgatc caagagcaca acttaattca gaaggctgaa tactgtggtt ttcctgagac
     7021 tccagcgtaa tcaacgccat aaacaacgtc tcgttaatcc cctgagcctt cagcatttta
     7081 ttgcggttct ccagcagctt gctttgcatg tgcatgcaaa gacgagtcag aaggatctcc
     7141 tgataaggaa aatcttcgtg gcggctggcg cgaaatttta gcatttgttc aatgggcgta
     7201 aacgaactat ccatttgggt atgacctcat taatttcagc cgatatagta acgacagtga
     7261 caaataaagt aaatgtattg ttttagaaaa atgattcttg tggattatat aatcgccatc
     7321 actttccaga cgagcccgta ggtcagcgca ctaagcagtg ttgggatgat aatgctgcgt
     7381 gttttataga aactggcacc cagtaccgcg aagccgacca gcgtgggcac gaaacggcgt
     7441 gtatcgtgca tcacttctgg tgcggtagag acaaccagca gagcgcatat cgaggcgatg
     7501 ccaatggtgt cgagcaaaat acctaccgcg ccacgtttgg ttgggcgggc attacccaca
     7561 cgcaggcgca gcggcaaata gcggaagcaa taattcgccg cgccaaccag taacccaagc
     7621 agcagaacct catagctcat cgggcgctcc ttgccagaat gcctggatta acgcagtgag
     7681 gcagccacag acaatgcctg ccagaatggc gacgggaata gaaaatagcg ttacgcctgc
     7741 aagggcacca actaacgctg cggtaacgca aagagattgt ttgcgctgga aagaggcgag
     7801 caggaaactc ataaagagtg ccggaagcat aaaacctaat gctgcttcaa cggcgggata
     7861 accttgcagc aagccgctgc cggagaatgc ccctattacc gtaccaaata cccacgatga
     7921 ccatgaactg aaggcaatgc cgatcatcca gttctcgctc cagcggcgat tattgcgtac
     7981 cagttttgcg gttgcggcgg caaaaacctc atccgtcagg ccaaacgccc acagggcggt
     8041 tttcgatttt tgcagacgct gaataatacg gctacgcagt gacgggccat acaacacatg
     8101 gcgaacatcc attgccatga cggtcagtgc agcaacccac aaactactcc cggctgccag
     8161 catcgcggta atgacgaact ggctcgcgcc tgcataaatg atgcaggaga aaaaaacgct
     8221 ttcgagagga gagaatccca gacgggtcgc attcagaccg aacgcaaagg ccaccggaat
     8281 ataactaata acaatcggta aactgtcttt gcatccttcc atgaaggtcg ccgaaccagg
     8341 agcaggctgt ggagtagggc tttccataga gtgtacgctt aacgattgtt aatattataa
     8401 atagactgaa tgaatatctt aaccttatca gactgatggg cttcttaaca cccttataag
     8461 tgtaaagcca cgaaaacggt tgctgattgc ttaatttgcg gcttcctgac gatgaaaacc
     8521 tcgccaccag accagtaagt taaccagggc aatcgtcgcg ccagccagac aaacgccagc
     8581 ccaaccgcca tgttgccagg ctgaggctga aattagcgaa ccggcggcac cgccaataaa
     8641 gtagctagtc atgtaacctg cggtcaggcg attacgcgca tcaggatgta tccgataaat
     8701 taccgtctgg ttagtgatat gcacgccctg cacggtgaga tccagcacca ggattccgat
     8761 aatcaacgcc agtacggaag tgtgaccaaa ccagatcgcc agccatgaaa gtaatagcag
     8821 cagcagaccg aaagttgtgg tgtggtgcga tttgccctta tcggcaaaac cgcccgccgg
     8881 acgagcgccc aacgctccgg cagctcccgc aagtccaaac agaccaatta caccatcgct
     8941 gtagttaaaa ggtggagcgg caagcaaaaa ggccattgag gtccagagaa tgctgaaatt
     9001 ggcaaaggtc aggcagccca gcaacgcgcg ggtacgcaga attttatcgc tgataaacat
     9061 actgaaaacg gaacccaaca actgtgggta gttgaggtgg gtttctgatt tcatttgtgg
     9121 cagaccacgc cataatgcca gcgccatcag tgccattaac accgaagcaa cccaaaagac
     9181 ggtgcgccag ccgccgagat tcgccagcaa tccggcaact gtccgtgcca gcaagatccc
     9241 caacagcaga ccgctcataa tagtgccaac cactttgccg cgtttgtccg gtgaagccag
     9301 cgtcgctgcc agcggaacca gaatttgtgc cacgacagag aataaaccgg ttaatgccgt
     9361 accgaggatc atcatcgcca gcgactgact gctggcagtg attaacatgc cgcctgccgc
     9421 cagtaaggtc atcgagacaa tcaggcggcg gcgttcaaac atatcaccga ggggaacaag
     9481 aaacagcaga cctgctgcat agcccaattg cgcggcggta acaataaagc ctgccgaact
     9541 ggcggaaagg gaaaagttac gcgcgatggt gtcgagcaat ggctgagcgt aatagttact
     9601 ggcgaccgcc agaccggtgg cgatagacat cagcacgatc agcgccgggc taagctcatg
     9661 attaggttta gtcattattt ttgttcatca ggtgatgatt aagctggaaa tcaataatag
     9721 cggatgttga tgaatatggg atgaaagata gcttgttgaa atgtgcgatg tggtttgtag
     9781 gcatgataag acgcgccagc gtcgcatcag gcattgtgca cagaacggcg gatgcggcga
     9841 aaacgcctta tccgccatac ggaatgagtg tattttgtag gcatgataag acgcgtcagc
     9901 gtcgcatcag gcattgtgta cagagcggcg gatgaggcaa aaacgcctta tccgcctgaa
     9961 taaaaattac ttctgcgctg ccagcgcctc attcacccag ccatcgaact gctgctggtg
    10021 ggctttgatc cagccatcaa cgtggccctg aatatcgcct tctgaggctt tgccgtcatg
    10081 cataatggcg ttctgggcgt taatatctgc cactggcaac tgcataatgg caaacagttt
    10141 cgctgctgcc gggtttttct cggcccaggc tttgttggca acgatatgca tggtgctgac
    10201 cgggaagcca taattcgcac cattcggcag tttggtatcg gcgtttttat cgcccggcag
    10261 tgcggagaac ggcacctgca accagacgac atctttgccc ggcttcagtt cgttactcac
    10321 ccagtacggc gtccaggtgt agtaaaacac cggtttgccc tctttgtagc gactgatggt
    10381 gtcggccatc atcgccgcgt agttcccctg attatgcgtc acggtatggg tcagttcata
    10441 cgcggcaagc tggtggttga tcgcaccttc gcagccccag ccagggttac aaccggttaa
    10501 atccgctttt ccgtcgccgt tggtatcgaa cagtttggcg atcttcggat ctttcagttg
    10561 tgcgatgttg gtgattttgt actggtcggc ggttttctta tcgatcaggt aaccctgtgc
    10621 cgcgccgtta acaaataccc cttcacgata aaatttctta tcgccaccgg cagcttcgta
    10681 catgttgtca tgcagtggcg tccagttcac ggcggtgaag gttgcatcgc cggaagcaag
    10741 cgaggtgtag ccaacgttgt aatccacttc gctgggtttg ttgacggtat aacctaattt
    10801 ctccagcgca cgactgacca gcagcgtctg gaaggtttct tcggtgatgg tgctttgaac
    10861 tggattaaca gtaatacctt tgcccggcag atcggcagca aaagtttgtg tagagataag
    10921 cgtggcaaac gctgtcgcaa aaagtacgct atgtcgcatt gttattcctt tttttggcag
    10981 ggtgataatg cccggcgtca ccgggcaagt gcagagttac ttaatgaatg ggcgggtcag
    11041 cagaccaaca gggccagtgg tgtaccagcg acggttgccg cgactgcgtg agtcgcgccc
    11101 aacggcctgc gtcaggcgat cgaggataat ggcgaggatc acaatcccga cgccgccaac
    11161 ggtggcaagc cccatatcca gacgaccgat accgcgaagt accatctgac ccagcccgcc
    11221 gacggcaatc atcgaggcaa tgaccaccat agaaagggcc agcatcagcg tctggttaac
    11281 gcccgccata atggtcggca tcgccagcgg taactgaact ttgaacagca tctggcgcgg
    11341 gctggcaccg aatgagcgcg aggcttcaat cagatccgcc ggaacctggt taatccccag
    11401 aatggtcaga cggataatcg gcggcagagc aaagatgatc gtcaccacca cgcccggcac
    11461 gttaccgata ccaaatagca tgacgattgg caccagataa acaaacgctg gcgtggtctg
    11521 catggcatca agcagtggac gaataatttt cgccgctcgc ggacttctcg ccagccagat
    11581 ccccaacggc aaaccgatga cgatacagaa cagcagggcg gttaacacca gcgccagagt
    11641 aaccattgcc tgcgaccagg caccgattgc gccgatggca atcagcgaaa ccagcgtcgc
    11701 cacgcccatt ccgaccccgg aaatctgcca ggcgatgaga gcgaaaacga taatcgccac
    11761 cggtgcgggc atacccagca gcaactgctg gaaaccgttg aggatataat caaccggaac
    11821 gcgcacgccc tggaagacgg gacggaaatg ggtaacaacc cagtcgatcc cttcagtgac
    11881 ccaactgtcg agcgggatca gcgttttatg gaacggatcg agaatattaa aatgctcgac
    11941 gtttggcgca ggcgtactgg tcagccagtc agcaccaccg ccgtcagtcg gtgcagtcgc
    12001 cggtgtaccc caggcgtctg cggattgcgc ggcactgtcc gccgctggcg tggtatccca
    12061 cggattattt tgatcagcca ttatttaccc cctcacgatc taaagcgcgc agcagcattc
    12121 ctttcgaaat gatgccgaca tactgttggt cctcgtcgac cacgggcacc gcacagggtg
    12181 cctgtccgac atgagagagc aactcgctaa gaggcgtttg tgcatcgact gctaacggcg
    12241 catcaatcag cgccgcatca agaccttgct gctgcgttaa cgcggtttta agcgaatcga
    12301 tggagactgc gccgacaaac ttattaccgc gttcgataac gtagccatat tcgcgatctt
    12361 catcctgcaa taatttcagt gccgaacgtg ggccgaagcc aggggtttta cgaattaagc
    12421 cattcggtgt ccggcgggca atatctttcg cactgaatac ctgactaata tcaacgccac
    12481 ggaagaaggt acggacataa tcattcgccg gattattgag aatttcatcc ggtgtgccga
    12541 cctgtaccac ttcaccattt tgcataatgg caattcggtc gccaatacgc atagcttcat
    12601 cgagatcgtg ggaaataaag acaatggtgc gctgatgttt cgcctgtaat tttaccagct
    12661 catcctgcat ctcggtgcga attaatggat cgagcgccga gaaggcttcg tccattaata
    12721 atatatccgg attaatcgct aacgcgcggg ctaatcccac acgttgacgc atcccgccag
    12781 agagttcatc cgggtagctg tgggcataat tttccagccc gacctgacgc agtgcatcaa
    12841 gggctttttc ccggcgttct tcggcattaa ttccggccaa ttccataccg aacgcagtat
    12901 tgtccagcac ggtcatatgc ggcattaagg caaaggactg gaagaccatc gcaatctttt
    12961 ttctgcgcac ctcacggagt tcggcatcgg atattttggc aatatccaca ccatcaatca
    13021 gcacttgccc gcgggtgggt tcaatcaggc gattgagaag gcgtaccatt gtggatttac
    13081 ccgagccgga taatcccatg atgacaaata tctcgccttc ttcaatggcc agactggcgt
    13141 cttttacgcc aagcgatagc ccagtttttt ccagaatttg ttctttcgaa agtccttgtt
    13201 cgatatattt gaacgctcgc tgtggatgct caccaaatat tttataaaga tttttaattt
    13261 ctaatttaat tgccatgcaa tagaaagatt cctttatttg tttatgtcga tatattaccc
    13321 aatggaaata gtcacttttt tctaccctaa catactgaga atctgaggca acccctgagg
    13381 gcaaatgtgg catgaatatt gatgcgagta aatcgccgga atttcccgtg atataagggc
    13441 tgagagcaaa tcgagaaaag ttgatatttt ttgtcaaaga tagcgaaaaa acgcctgaat
    13501 attgtggttt caggcgaata atgcaacaga gtatcagtgt agatcaccac aaaatatttg
    13561 cgtgataaaa agctatttgg cgggaattat ttccctgtcg cgggtagtga tatttttgaa
    13621 aataacaccc tcagaaattc cagtcttcat cttctgtttc aaccgctttc cccatcacat
    13681 aagaggagcc tgaaccggaa aagaaatcgt gattttcatc ggcattcggc gaaagcgcgg
    13741 cgaggattgc cggattcact tccgccattt ctgcgggaaa taaaggttcg tagcccagat
    13801 tcatcaaagc cttattggcg ttgtaacaga gaaacgcttt cacatcgtca gcccacgggg
    13861 tttcggcgta cagctcatcg gtgtattgca actcgttgtc gtagagttcc agcaacaaat
    13921 cgaaggcgaa actcttcaac tcttcaagtt gtcccagaga tatcttttcc atgtttttct
    13981 gatatttata gcctatgtag taaccgtgta ctgcttcatc gcggataatc agacggatca
    14041 ggtctgcggt attggtcagc tttccgcggc tggaaaaata catcggcagc cagaaaccgg
    14101 aatagaacaa aaaagattca agaaacacac tggcgatttt ctttttcagc ggatcatcac
    14161 cgcgataatg ttgctgaata atctgagctt ttcgctgcaa cggtgcgttt tcttcactcc
    14221 aggcgtaggc ggcatcgaca tctttggtct ggcacagcgt cgagaaaatc gaactgtaag
    14281 agcgggcatg aaccgcttcc ataaagctga tattcgataa taccgcttct tcatgaggcg
    14341 tgagtgcatc gggcatcagc gaaggcgcgc cgataacatt ttgcagcgtg tcgagcagcg
    14401 tcaggccagt aaaaacgcgc atcgtcagtt gttgttctac gacagttaat gtctgccagg
    14461 caggaatatc gttcgacagc ggcacctttt ctggtagcca gaaattgctg gtcaggcgat
    14521 tccatacctc cagatcttta tcgtcagata tcttgttcca gttgatggcg ctgatacgtg
    14581 agagtttcat agatattcct taaagtgcac aggagacgca gccttcaatt tcagtgcctt
    14641 ccagcgccat ctgacgcagg cggatgtaat agagcgtttt gatacctttg cgccaggcgt
    14701 atatctgcgc tttgttgata tcgcgagtgg tggcggtatc ggggaaaaac agcgtcagcg
    14761 acagcccctg atcgacatgg cgagtcgctt ccgcgtaggt gtcgatgatc ttttctgcgc
    14821 caattttgta agcgtcctga tacagcgcca gattttcgtt agtcataaac ggggcagggt
    14881 agtaaacgcg tcctgttttg ccctctttgc gtatctctac tttcgccaca atcggatgaa
    14941 tactcgacgt agcatggttg atataagaga tagaaccggt tggcggcacc gcctggagat
    15001 tctggttgta tatgccgtag cgcatcacgt cgtcgcgcag ttgcgcccac atctcacggg
    15061 taggtaacgt aataccgctg cgggcaaaca gttcgccaac tttcgccgtt ttcggctgcc
    15121 agttaccttg cagatattgg ctgaaatatt caccactggc atagcgtgac tgtttgaacc
    15181 cggcgaaggt ttcaccgcgt tcgcgcgcca gcaacatcga ggtacgcagt gcgtgccagg
    15241 tgatggtata gaaatagaga ttggtgaaat ccagggcttc cggcgaacca taagcgatgc
    15301 cttccctcgc cagataacca tgtaaattca tctgccccag tccgatggcg tgcgaggcgg
    15361 catttccggc ttcgatggac ggcacgctgc ggatatgact catatctgac accgctgtta
    15421 aaccacgtac ggcagtctct accgtgcggg caaagtcggg ggaatccatg gtgtgagcaa
    15481 tattcaacga acctaaattg caggagatat catggcctgt gcgggcatag tcgagattct
    15541 cgtcatactc tgaggcacag ttgacctgca aaatctctga gcaaagattg ctcatattta
    15601 tgcgcccggc gatagggtta gcacggttta ctgtgtcttc atacatgatg tagggatagc
    15661 cggactcaaa ctggatttct gccagtcgct ggaagaaatc acgggcgttg aggtattttt
    15721 tgcggatgcg ttcatcggca accagttcgt catagtgttc gctaatggcg atatcggcaa
    15781 acggcttgcc ataaactcgc tcaacgtcat agggcgaaaa cagcgccatc tgcgcattct
    15841 cttttgccag atggaaagtg atatccggga tcaccacgcc aagcgacagt gttttaatgc
    15901 ggattttttc gtcggcattt tcccgtttcg tgtcgagaaa acgcagaata tcgggatgat
    15961 gagcatgtaa atagactgca cctgccccct gacgcgcgcc gagttggttg gcataggaaa
    16021 atgcgtcttc caacattttc atcaccggaa ttacgccaga agattgattt tcaatacgtt
    16081 taattggcgc gcccgcttcc cgcagattcg acagcaaaaa tgctacgccg ccgccgcgtt
    16141 tcgacagctg cagtgcggaa tttaccgccc gaccaatcga ctccatattg tcttcaatac
    16201 gcagcaaaaa acaggaaacc agttcgccgc gctgctgctt accgcagttg aggaatgttg
    16261 gcgtggctgg ctgaaagcgt cctgacagca tttcatcggt cagttgcaac gccagcgtct
    16321 catcgccttg tgccagcgtc agcgccacca tcgttactcg atcggcaaaa tcttccagat
    16381 aacgtttacc gtcgaatgtc ttcaacgtat agctggtgta aaacttccat gccccgagga
    16441 atgtctggaa acgaaaaccg ctggtgtgtg cgtgggcaaa cagcgtaatg acaaagtcgc
    16501 gagagtagcg attaagaacg ctttcatcat agtaaccttc gttgaccagc cagttcaggc
    16561 gctgctgctg gctactgaag gtcacactgt tcggacgcac atgcgtcgca ataaaggcgt
    16621 caacggcctg gcggtcttta tcgaactgaa tgcgacctgc gctatcgtag aggttaagca
    16681 tcgcattcag cgcgtggtaa tccatcgttt cctgcgtcag gcattctgcg gttgtcgttg
    16741 ccaaaattcg gttactcctt tacgaacgtt ttcgatatcg ctttgcgtac ccatgagttc
    16801 aaaacggtac agccacggca cgccgcattt ccgggcaatc acatctccgg cgcggccata
    16861 cgcctcacca aagttgcgat taccagaagc aataacgccg cgaagcaacg cccggttgtg
    16921 ctcgtcgttt aaaaagcgaa ttacctgtcg tggcaccgcg ccagccgtac cgccgccgcc
    16981 gtaagagggc acgatcagga tgtaaggctc gtctacctga atccgttccc gctcattgag
    17041 cgggatgcgc accgcgggca gacctaaacg ttcgataaaa cgctgcgtgt tttcggagct
    17101 gctggagaag tagacgagct ggctcatgca ctggccgcgt gtggcgctgg atgcagacgg
    17161 ttaatcatgt ccggacggaa accagaccag ctaagatcgc cagcaatcac taccggcaac
    17221 tgacgaaagc cctgagcacg caacgcttct gccgcttcag gaacgcgatc gacattaatc
    17281 atttcaaaat caaagccccg attttccatc gcccgtttgg tggcgtggca ctgaacgcaa
    17341 tcgttacgag tgtaaatagt aatgcgcatg attcgtattt ccgtttaaaa tgaagatacg
    17401 gcgcgatgat acgcgtcggg ttgtctttct gttgatacag agatactaga tgtagttgaa
    17461 aaaagattca accacacaat atatagcaaa tagtggtcga aattaccctg gatatgagcg
    17521 tgacggggta gggggatttt tgtgattcac caggcaaaaa gaaaccccga agacaggctt
    17581 cggggttaaa gacgcgtatt tattatcatt tttgcactac gatttgcgca tgcttaacag
    17641 tgcgccgata aaaataccta ccgcagctgc tgtacccacg ctacaccagg gtctttcacg
    17701 aacaaaagaa tccgcgcagc caacggcatc gcgtgcggct tgctggacgc gagtacgacc
    17761 atgcattcgc gccctggttt ctttcagcaa tgcctgagct ttactgcgtg cggcttcagc
    17821 ttcccctttg gcgtcgctgc cccaggactt caatacagat tcgaggctgt cagctaattg
    17881 attgacatca ttctggatat cctgcacgcc atcatcaacg tcgttgcggt tcggtcggtt
    17941 aaacatatgc tcccccatta gaattcgatg taattttcag tgtagaccat aattttctta
    18001 actgaggttc atcgcggctt tatggacttt tctgaaagcg ttgtgaatgc gcaatcaggt
    18061 aaggtaggcc cgcggttgat aataacgagg atcaaatatg tatttacgac cagacgaggt
    18121 ggcgcgcgta cttgaaaaag tcggttttac tgtcgatgtg gtaacccaga aagcgtatgg
    18181 ttaccgccgt ggggaaaatt atgtctatgt taatcgcgaa gcgcgaatgg gacgtaccgc
    18241 tctggtaatt catccaacat taaaagaacg cagttcgaca ctggctgaac ccgcttccga
    18301 tattaaaact tgcgatcatt accagcaatt tccgctctat ttagcaggcg agcgacacga
    18361 gcattacggt atcccgcatg gctttagttc gcgtgttgcg cttgaacgtt atttgaatgg
    18421 tttatttggc gaagccagtt aaagacgcga ctggcgatgc cagtcgcgaa aagaagagtc
    18481 aggcttttac ctgctggtaa cggctgactt taaacagtcg gcggcaataa tcgaggaagt
    18541 agccataaac cgcccccatc aacatcgaaa caacgatgtt tgaactgacc gccgccataa
    18601 tctgatgcca gtctgcgccc accactaaca agatcgccac atacaccggt gactggaacg
    18661 tcacataagc caggatatcc gccagatttt ttatccagcc cgacgggcta actttgcgtg
    18721 ccgcgcgcat aaacagatca cggtacatac cgtatggcca tgcaattaag atgttcaccg
    18781 gaatcgctac caatctggaa taaaaagact gttcgaagct cattccggag aggaaaactt
    18841 caatacacat gttcacgaca gaacagtaaa caaccatcgc gaacgtatct gcaactgcat
    18901 gacgcaagcg tgactgcggt gagaacatgc taatgctcct taataaaagc gtaatgggat
    18961 gttaatggag atgaaaacag tggatctcat tgccatctgc attttcgcat agtgtatatc
    19021 gctggctatg actaatcaat tagtgaaaaa tttttattta gtcagttttt gtagataaaa
    19081 tattccgtaa cggctcgttt tttgtacatt gtggattttt acatgccgat atgatttatt
    19141 ttcaattaaa tcaattagat atgacctttc ctttaatttc actaatgaaa gtttgggggt
    19201 gaggtaacgc tataagcgta gagaattcgg tgtatgtcgt gaccgtgtca tttacagtat
    19261 tttttatgcc agaaaatgta cattaacagc agaaagcacc agtgaggggt gttttctttt
    19321 attctgttat attgcatttt tattcacttc ggttttgtca ggcttgcgga attagcgagc
    19381 agagagcgcc gttctgttca ccaataatct aaattactca tcaaaatggc taatatataa
    19441 tcttgaaatt attctgagag tttcagaaaa taacagctga aataatctcg cgcaggactg
    19501 taaatagatt aaattttgtg gaaatataat aagtgatcgc ttacactacg cgacgaaata
    19561 cttttttgtt ttggcgttaa aaggttttct ttattatgtc cgtaatgtta caaagtttaa
    19621 ataacattcg cactctccgt gcgatggctc gcgaattctc cattgacgtt cttgaagaaa
    19681 tgctcgaaaa attcagggtt gtcactaaag aaagacgtga agaagaagaa cagcagcagc
    19741 gtgaactggc tgagcgtcag gaaaaaatta gcacctggct ggagctgatg aaggctgacg
    19801 gaattaaccc ggaagagtta ttaggtaata gctctgctgc tgcaccacgc gctggtaaaa
    19861 aacgccagcc gcgtccggcg aaatataaat tcaccgatgt taacggtgaa actaaaacct
    19921 ggaccggtca gggccgtaca ccgaagccaa ttgctcaggc gctggcagaa ggtaaatctc
    19981 tcgacgattt cctgatctaa ttactgaggc cggatgtcgc tgctagggcg cgtccggcgt
    20041 cgcttctcaa gctcgttggt tccacggcat cttatctaac aaccttgcca ttccacaaaa
    20101 accgctgatt cctgcaaaca gtaacccggc accaacaaag ccacttaata agaagaaacc
    20161 gctatttacg gtataaccca gtacaacgcc gattaatatt aaaccacccg cagcgatctg
    20221 cacctggcgc attaacggaa agggttgaga tttatttacc gctactggca atccagctct
    20281 tttccagcca tcaatcccat cttcgagtaa aaatatttct gcgggggcgg caatcgctgc
    20341 taatttatcg gcattattac tggtgcgttt acctgcctgg cagtggaata taatttgctc
    20401 gtgacgtaat ttagccggaa gacctgactg ttccagcacg gataatggag ccagatctgc
    20461 ttcaggaata tgttcacgaa gatattcatc agcatcacga atatcgatta actttgcgcc
    20521 gcgtgcgatt aattcttgtg catcatgcgg cgaaatggtt gtcaaagcca tagtgactcc
    20581 ttacggacaa tagacatttt tcagggtggc gataatggca tttaccgcct catttttaat
    20641 ggaatataga atgcgttggg catcccgttg gctgtcgata agcccttcgt cccgcatacg
    20701 agcgagatgc tgtgatgtcg cagaggcact cagtccggta atgcgcgtca gctctcccgc
    20761 gctggtgccg ggggaaccgc taagcatgca cagaatcagc aatcgtttag ggtggctcat
    20821 tgctttcaat aaggccgctg cctgttcggc actggcctgt aattgcgcga gttcagtcat
    20881 atttatttaa gtattttcta aattaagtaa actctaaact aaaaatgcaa catataccag
    20941 cctcagcagc gtaaatgaga gtaaaagcgt aagctgaaac tggcaggctc cgctaaaatt
    21001 actacgctta agagataaaa tctcttttta aacaatgagt aattttctta tagggagtac
    21061 atatgggttt ctggagaatc gtcatcacca tcattctgcc gccgctcggc gtgctgctcg
    21121 gtaaagggtt cggttgggcg ttcattatta atattctgtt gacactgctt gggtatatcc
    21181 ccggtttgat tcacgcgttc tgggtgcaaa cccgcgatta aacctgtgct catccccgcg
    21241 gcgttaacgc gccgcgttcc tctgctacac tttctgagtg tttccgttaa cgaatgaggt
    21301 agctatgggt ctgttcaatt ttgtgaaaga tgccggagaa aaactctggg acgcggttac
    21361 aggtcagcac gataaagacg atcaggcgaa gaaggtgcag gagcatctga acaaaaccgg
    21421 tataccggat gccgataaag tgaatattca aattgccgac ggcaaagcga cggtcactgg
    21481 tgacggcctg agtcaggagg cgaaggagaa aatccttgtt gcggtgggga atatttccgg
    21541 tattgccagt gtcgatgatc aggtgaaaac ggcgacacca gccactgcca gccagtttta
    21601 taccgttaag tctggcgaca ctctgagtgc catttccaaa caggtctacg gtaacgctaa
    21661 tctgtacaat aaaatcttcg aagcgaataa accgatgcta aaaagcccgg ataaaattta
    21721 tccggggcaa gtgttgcgta ttccggaaga gtagttaatt gccagccatc gcctgctgga
    21781 taatgggaat tggcgtcagt aaatgctggc gcattaactc actggcgcgc gcggtatctc
    21841 gcgccagtac cgccgcagtc agggtctggt gctgatcgtg tttatcctcc agcatttcca
    21901 ccgaaagcac cgttcgccgc agccagataa atcgataacg cgccgccaga tcaaacaacc
    21961 gttcacgcat ttgcagcaaa tagtgagaac cacagcccgc cacaattgcc gtatgaaacg
    22021 cctgatgacg cagatcccac tcatcaagca ttttctcgct ggcgtcacag gcctcaagct
    22081 tactcagcag atgcgctttt gcgagaacgt ctgcctccca ctcatcgcca ccgcgggcaa
    22141 tcgccagact cactaacatc gcttccatat tggcgcgggc gtcgaaaata tcgagcagct
    22201 cctgctctga catagacgcc acccgatacc ctttttgatt caccaccgtg accagccgtt
    22261 ccgccaccag ttgcgaaaga gcttcccgta acggcccaac gccaagtgca taacgcgatg
    22321 tcagcaaact cattcgtaat ttttcatccg gttgaaaatt accgcgaata atatcgttct
    22381 tcagccagcg atagccatcc agagacgtaa tggtcatggt gtgactcctg atcatgatca
    22441 gcgcgtatta tgaacgggtg ttttttgcca caataccagc tttttccagc gcgccataat
    22501 tggcacggta cagataatcc ctatcgctaa taagccggtg gagatcactt ctaactgttg
    22561 cgccggacgg aatagcatca ctaccaacac aaaggtaata aagccgatga ccagccaggt
    22621 gagccacgga taaagccaca tgcgcaagcg aatttcgctt ccttctgctc gcagaatttt
    22681 gcgcatccgc aactgtgaaa cggcgatgac taaataaacc agcagggcga tagcgccgga
    22741 gctgtcgatc agaaatttaa ataccttcgc aggcgcgtaa tagttcacca ccaccgttaa
    22801 gaatgccgcg ccggtggaga gtaacaccgc cacgtacggg gttttactgc ggttgatttt
    22861 acccattacc gcaggcgcat ctccgcgacg gcttaaggag tagagcatcc tggaagcggt
    22921 atacagcgcc gagttcaggc aactggttac ggaaagtaat atcacgcagt ccatgatcaa
    22981 tttcgcatgg ggaatattga gcaattccag taccgagcga taagaaccga cggcttttag
    23041 tccgggcata ttccacggaa ttaatgccac gacgacaaaa atagaacata aatagaagat
    23101 agaaatacgc cagataaccg agttggtggc gcggacaata tgtttttccg gcgtgtcgga
    23161 ttccgcggcg gcaatggtga caatttctgc gcccataaac gagaacatgg tgatcaacat
    23221 cgcgcttaat accgcaccga aaccgttggg cataaagccg ccgctatccc acaatcttga
    23281 gatcccgctc acttcggcat aagggtaaaa accgctaatt gcgactgcac caaggaaaat
    23341 aaaggccagg atagcgatga ctttgcacag cgccagccag aactcaaatt cgccgtagtt
    23401 tttaacgctt aataagttac tgccagttaa ggcgagggta atgacgaggg aaaataacca
    23461 gatgggaatg cctggaaccc atgagtgcag gatcatggcg gcgatgttgg cttccagcgg
    23521 gataaccagt acccaaaacc accagtacaa ccagccgatg gtataacccg cccaacgacc
    23581 aatagcttta tcggcatagg tggaaaacga accggtatcg ggcgtggcaa ccgccatttc
    23641 cgccaacatc cgcataatca taaccaccag tagcccggcg aacagatagg ccagtaatac
    23701 cgccggaccc gcttcggcga tggcgacgct ggaaccaaca aacagacttg cgccgataac
    23761 accggcaata gacaacatgg tgacgtggcg tgacttcagc ccgccgccta actcatgtgg
    23821 ttgcgatgat tgccccatcc tgaatcctct cgaaagtgtg tcacttggga gagcgccccg
    23881 acatttcccg cgtcgggtca cgcgtataat tgttattgat tctttttgcc ggagagcggc
    23941 tccgccttac ccggccaaca tagatttctt cctgttcacg tacagattgt cggatgcggc
    24001 gtaaacgcct tatccgacct ggatccccgt aggccggaca agacgcgcag cgtcgcctcc
    24061 ggcataggag cggcgctact gcttcgcctc atcaaaacac tggctgatga tctccagacc
    24121 ctgacggatc tgagcgtctt caatggtgag cggtacaagg atgcgcagca cgttgtaata
    24181 cgggccgcag gagagaagaa tcaggccttt atcgcgggcg cgagccacga tctcggcggt
    24241 gagtttggcg tccggcttgt tgtgatcgcc gtcttcaaac agctcaatgg cgatcatcgc
    24301 ccccagcccg cgtacgtcgc cgatctccgg gtgtttttcg gctatcgcca gcaatccgtc
    24361 tttcaacttc tgccccagat cgttggcttt ttgcagcaga ttttcctgct caaacacctt
    24421 caacacttcc agcgcagcca cgcaggcaat cgggttaccc gcataggtgc cgcccagacc
    24481 gcctggagcg acggcatcca tcacttccgc gcgcccggtg acgcccgcca gcgggaagcc
    24541 gcccgcgatc gatttcgcaa aggtggtaag atccggcgca acgcccatct gctccatcgc
    24601 aaacagcgtg ccggtacgcc ccgcgccgct ctgcacttca tcggcaatca gcatgatccc
    24661 gtgctcgtca cacagagcgc gtaaacgctg cataaaggct ggcgacgcgg cgtagaaacc
    24721 gccttcgccc tgaaccggct caatcacgat ggcggcgata tcttccggcg cggcatcatt
    24781 tttgaagatc cggtggatgc tggcgatagc gtcatcctcg cttatgccgt gcagcgggca
    24841 aggataaagc gcgcgataaa catgacccgg catcagcccc atgcccgcag agtacggatt
    24901 caccttgccg gtcagcgcca gcgtgtaatg cgtgcgcccg tgatacgcgc cgctaaaagc
    24961 gatggtgccg ctacgtttgg tggcggcgcg ggcgattttc accgcgtttt ccaccgcttc
    25021 agaacctgtg gtgaccagca gcgttttctt ggcaaaattg cctggcacct tctgattcat
    25081 aatctcgcac agctccagat acggctcgta ggccagcacc tggaagcagg tgtgcgacag
    25141 ttttttcaac tgcgcttcca ccgcagccac cactttcgga tgcagatgcc cggtattgag
    25201 caccgcaatc ccgcccgcga aatcaagata ctcacggcct tcaacgtccc acacccggca
    25261 gttttccgcg cggtcagcga aaatggggtg aatttgccca acgccacggg gaatcgcctg
    25321 actgcggcgc tgcattaact ctttattgct gctcattcgc gttctccagt taaagaccga
    25381 tgcacatata tttgatttct aagtaatctt cgatgccata cttcgaacct tcacgaccca
    25441 gacccgaggc tttgatgccg ccgaacgggg ccacttcatt ggaaataatg ccggtattga
    25501 tgccgacgat gccgtactcc agcgcttcgc ccacgcggaa gacgcggctt aaatcacggg
    25561 cgtagaaata ggcggcaagg ccaaactcgg tgtcattggc ttgcgcaatc acatcggctt
    25621 catctttaaa gcggaacagc ggggcgaggg ggccgaacgt ctcttctttc gacactttgg
    25681 cgttggccgg aacgtccacc agaatggttg gctggaagaa gttgccgccg cgttcgtgcg
    25741 ctttacctcc gcaaaccacg cgcgcgcctt tctccagcgc atcggcaata tgctcttcca
    25801 cttttgctac cgctttttca tcgatcagcg gcccgatggt gacgccattc tccagcccgt
    25861 cgccgatgtg cagtttgctc accgcctgct gtaatttttc ggcaaagcgg tcatacacgc
    25921 cgtcctgcac gtataaacgg ttggcgcaga cgcaggtttg cccggcgttg cggaatttcg
    25981 aggacagcgc accttccacg gctttatcga gatcggcatc gtcaaagacg ataaacggcg
    26041 cgttgccgcc cagctccagc gacacttttt tgatgtcttt cgcgcactgt tccattaact
    26101 ggcggccaat ttcggtcgaa ccggtaaacg acagtttgcg caccagcggg ttgctggtca
    26161 gttcgttacc gactgcgccc gccgaaccgg tgaccacgtt aaatacccca gccggaatgc
    26221 ccgcgcgaat cgccagctcc gccagcgcca gcgcagagaa cggcgtctga ctggcgggtt
    26281 tcagcaccat ggtgcagcct gccgccagcg ccggaccggc tttgcgggta atcatcgccg
    26341 ccgggaagtt ccacggcgtg atagctgcgg taacgccaat cggctgcttg ataacaatca
    26401 ggcgcttatc ggcctgatga ccgggaatgg tgtcgccata aatgcgtttg ccttcttcag
    26461 caaaccactc aataaaggag gcggcgtagc taatttcgcc tttcgcttca gccagcggtt
    26521 tgccctgttc tagggtcatc aggcgcgcta aatcgtcctg atgctccatc atcaaattga
    26581 accagttgcg cagaatgttg gcgcgttctt tggcggtgag cgcacgccag gcgggcaggg
    26641 cgcggttggc ggcgtcgata gcggcgcggg tttcatcagc gcccattttc ggcacgctac
    26701 ccagcttgtc gccgttcgcc ggattggtga cgtcgatgac ttcaccattg ttggcgtcca
    26761 gccattcccc gttaatcaac gcctgctggc ggaataagtt actgtcgtta agtttcattg
    26821 ttacgtcctg ttttcaaagg ttattgatta aatgcggcgt gtaaggcatc cacactacgt
    26881 gccgctcgca gcgtgcgtcc ggggttactc tggcttgcca acagcgtttg taccttgctg
    26941 acaatatgcg caccaatagg aattgctgat gtcgctgccg gggagggcgc attgcaggtg
    27001 tggatcgtgc gcggggtggt gacaaacaga aaatcgtcaa tcaacttgcc atccggcgat
    27061 accgcctgcg cccgcacacc ggcgggccag ggctggagat cgcttaacga aagccgggga
    27121 caatactttt gcaccagccg cagatagccg cttttgcaca gcgagttttt catctcgccc
    27181 agtcctgagc gtagatggtt ttgcagcacc cggcgaatcc ccgacgagcc taaaatttcc
    27241 agcgtgtcgc taaacgagaa gtcgcgcttg cggtagcctt cgcgtttaaa agccagcacc
    27301 gcgtttggcc cgacggtcac gctgccatcg atcattcggg tgagatgcac gcccaaaaac
    27361 ggcattgccg ggtcgggaat agggtaaatc agatggttaa caatctggtt atgctccggc
    27421 gcaaggcgga aatactcgcc gcggaacggg cagataataa agcccggttc gaggccgagc
    27481 attttcacca gccggtcagc catcagcccg gaacagctaa tcagcgtcga tgcttcatat
    27541 tcgccgccct ggcgggtacg tatcaccacg ccgtttttat gctcactgag gccgctgact
    27601 tcggcgttat agataatctc accgcctctg gcctggaaga tttttgccat tgccgccgtc
    27661 acgttgcggt agctgacaat gccgctggat ggcacaaaaa tgccgccaag cccggtgata
    27721 ttcggttcgc gctcgcgcag ttccatggcg tttaaccact cgcgctcgat accgtttgcc
    27781 gccgtgcgtt cccacaacgc gcgcatccgt tccatttcga gttcagaggt ggcgaccagc
    27841 atcttgccgc agttgtcata gcgaatgccg ttttgatcgc aaaaggcttt ggtggcgcgg
    27901 tttcccgcca ggcaaaactg cgccttcagg ctaccgggcg tgtaataaac cccggcatgg
    27961 atcacgccgc tgttgtggcc cgtctggtga caggccgggc cggactcttt ttccagcaac
    28021 gcaatgcggg catccggata aacatcaatc agttgcatgg cggtcgacat gccgatgatg
    28081 ccgccgccaa taatcacaaa atcatacatc cgctcaattc ctttgcgctt actgatgcgt
    28141 ctggtagtgg tgagtggcgt aagcgaaata gccacgctga cgcatcagtt cacggcgcag
    28201 atccgggtgc ggagtaaagc ggtcgcgacc gtgcagccag aacaggttgt ttatcaacag
    28261 gaatttgcca acgggaacgg gtacagaaag aatgcctttg ctggtttcaa tggcatcgga
    28321 aagctcgctc aaccacacgc cttcttcaaa atcttttggc tggacgaact ggtcgatata
    28381 gcgcatcacc gggcgaccct gttgatcaac gtcgaacact ggatggaaaa catctttgct
    28441 gacgtttttg ctcggcggcg cggcaaagcg catcgggcga cgcgccagtg gatggcggaa
    28501 atagtggtcc agatgttccc agtcatcgag atgcagcagc aacgaatttc caccctgcat
    28561 gttttgctca tcgattttca tcatcagcac gtaatcggtg atctcttcga catacgtgcc
    28621 gtcgttgtgc agttccatca cgcggtgcgg ctgacgcaga tagctgtctg agttatcgac
    28681 atttttcacc acgaaacgcg cgtaatactg accgctcatc gcatcgaaat ttgagcgacc
    28741 aatcagatgc gctaccgccg ttgccagctt caccatctca tctgcctgcg ccacatcatc
    28801 gatacccacc gcattgatca acagcgcacc ttcagcgcgg tttagcaggg tcttcaacag
    28861 caatggttgc agctgattcg cacacagatc gtcaagaatt ttgcctaccc gaaaacgcag
    28921 aaacgatttg tactccagcg cctgcacggg ccattcggca acctgttcga gaaactgttt
    28981 ggtcgtctgt tcggtgaagg tgagttccag cagacgcggg gattgcgccg acgggatgag
    29041 agtgaatccg ctatagtcct ggcctgaatc gacagcgtta ttttgtacgg cggtcagtgc
    29101 attcatcaga agcgatcctc ttatgagatg tagggtgaca tggcgatgct catttcgtag
    29161 ccataatcta aaaatatcta catttctgaa aaatgcgcac aaaagcgaca tattgttttc
    29221 ttattgtgat caaaagcaat aaattagtaa catttaacgt agggtattaa ggcaaagtga
    29281 gacaggaaat gaataactaa agcaacaggt tagggagagt taacattaga gaacagatta
    29341 tctggtcgat aaaaaacagt aaatggcttc actgcaaaat gaccgttagt aaactaaaca
    29401 tattatctgc cccgttttat cggggcagat ataacattaa attataaata gagatagttc
    29461 agcggttctc ctgttttccc taaaagaaga gcgctacgcc caggaatatt gatatttaga
    29521 gttccgcctc taagcgtaaa tactttacca ggggggataa tttttcgggt attattaata
    29581 ataatgttaa cgctattact ccccatgttt ttgtatattc caggttgcaa tttgttaggc
    29641 caggtacctt caacttcttc gtttccttta tttattataa ctagtccatc atgaggagag
    29701 tgattaatgt tcacagaatc cccacgacta aaggccagaa cgtttttagt tgcagataca
    29761 ccctcaaatg cgctccatat tcctgcttta tctatatata atgcgtggaa ttcattgtag
    29821 agctgctgat aggtagagca acctttatta agctacgcct caccgccgac acgcgtggac
    29881 ccatcataat ttttaatttc agtcgttgat tttaatactt ggccatccca gtaaactaaa
    29941 ggcctgctga acggcagagc ggctatatat gccattgcta attgttcatc ttgttgatgg
    30001 ttaaatagaa agtcattgaa aagattacca ttgttaggaa aatcgtggtt aatgttaatc
    30061 aatatccgtc tttctgatgg gatacttgag attgtggaac ccggttttga tgcaagggtt
    30121 tgaagatctc caccaaatga aaaggctttt atcgttttca tgagcaatga aaaatcaatg
    30181 gcgcttaaat catctctttc ataaagcagt gatgacattt gttgttcatc caactgacaa
    30241 gttaccagat attccatgta attcataacg gcttttttat gatatttttg gttaaataag
    30301 ttagtattgt gtaaccgctc attataattt ttaagcggtg gggttattga tctttctatt
    30361 tgttcatgtt ttgagtgttt tgccgcatca taacgtaaac ctcgtacgcc atattttcgc
    30421 atttcacgaa cgtattgatt ttgcaacata ataacctggt cacttttggg tgttgtttta
    30481 ggcattccat ttaaacgctt ttcttttaat gcatggaggt tttcccattc atccttagag
    30541 atgttatctt tacttgcttc gaagtctttt gcagtgagat gtatatcttc tggatggatt
    30601 tgtttacgtt cttttgttac tggatcttta atgaatggaa tcaaaacctg accttgccaa
    30661 tagccaggga taccaccttc tatatcttta ctgatcttcg ctcgtatatc agcagaggga
    30721 taatccaggt catttttacc actaccccca attcccataa agttaaaggt ataatcagga
    30781 atgatatcga taccaaattt tttagcatct ttaaccaatg caataaaatc ctgcaaatct
    30841 cccattggat tatggatggt acaaagatct tcgggttgat aagcatgaaa ccagtaaccc
    30901 ttttcttctt cgggagtttc tggtccgtcg gcgcggaaag ttgtcactgg agggatttgt
    30961 atatacccat aatggcaacg tgcaaactca gacatatttt ccctgatttg ctggtaggtg
    31021 gcattaaaag catgaagaat cgcttcgcca ggttttttgg gggttatgat ttcacattgt
    31081 gatttctgaa cagcagtaag agatttacgc tcaagtgttt gccgtagatg ttctggattg
    31141 attagtattt taaatgtttc ttgctgttcg gaaggtgacg tcacgttaat attatataca
    31201 tcggctgcca gctcatagtc aggaatgtta atagcatatg caatatactc atcttttggg
    31261 gacggtttgt cagatataat gtgggtgaaa tgttcagcag gaccatactg atgaacaccg
    31321 ctgttatcac ctctttcaat cattgtatct ctttgataaa cccatgattt tccttctcct
    31381 atcctgtctg gcgctgtaat gctacgctgt gctgctttat tcaactctcg ataagttgta
    31441 tctggagaaa gggaaatttt ataatcgttt tttaacagat cattaataac tttttctgcg
    31501 tgaaaagttg atataatttc tctgtctttt aatccttgag ggtaaactcc acaagaatta
    31561 ataatatttg ttatacagtt tagatattct aatgtattta atttggaatt tagatccccg
    31621 tcagtaatgt tccatgataa caatgtgggg ttgtcgatag gtaaatttct gggtcctggt
    31681 tttatagaaa acataataaa gttcctctaa tggtagagta atatgatgta tacggaaaca
    31741 taaaaatgtt taattgttgt gaaagtaatt gcaaacagat gtcctacttt tatccaatat
    31801 ttatatggaa aaggataatg cttaaatctg ttttattaat tatcgatgtg gtctggagag
    31861 g
//
LOCUS       DAMZET010000034        31054 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.034, whole genome shotgun sequence.
ACCESSION   DAMZET010000034 DAMZET010000000
VERSION     DAMZET010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.034"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..204
                     /gene="tuf"
                     /locus_tag="POB34_001696"
     CDS             <1..204
                     /gene="tuf"
                     /locus_tag="POB34_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDI5918665.1"
                     /translation="RPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDD
                     GLRFAIREGGRTVGAGVVAKVLS"
     gene            434..817
                     /gene="secE"
                     /locus_tag="POB34_001697"
     CDS             434..817
                     /gene="secE"
                     /locus_tag="POB34_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDI5918666.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            819..1364
                     /gene="nusG"
                     /locus_tag="POB34_001698"
     CDS             819..1364
                     /gene="nusG"
                     /locus_tag="POB34_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDI5918667.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1523..1951
                     /gene="rplK"
                     /locus_tag="POB34_001699"
     CDS             1523..1951
                     /gene="rplK"
                     /locus_tag="POB34_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDI5918668.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1955..2659
                     /gene="rplA"
                     /locus_tag="POB34_001700"
     CDS             1955..2659
                     /gene="rplA"
                     /locus_tag="POB34_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDI5918669.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3072..3569
                     /gene="rplJ"
                     /locus_tag="POB34_001701"
     CDS             3072..3569
                     /gene="rplJ"
                     /locus_tag="POB34_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDI5918670.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3636..4001
                     /gene="rplL"
                     /locus_tag="POB34_001702"
     CDS             3636..4001
                     /gene="rplL"
                     /locus_tag="POB34_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDI5918671.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4321..8349
                     /gene="rpoB"
                     /locus_tag="POB34_001703"
     CDS             4321..8349
                     /gene="rpoB"
                     /locus_tag="POB34_001703"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDI5918672.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8426..12649
                     /gene="rpoC"
                     /locus_tag="POB34_001704"
     CDS             8426..12649
                     /gene="rpoC"
                     /locus_tag="POB34_001704"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDI5918673.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12862..13401
                     /locus_tag="POB34_001705"
     CDS             12862..13401
                     /locus_tag="POB34_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HDI5918674.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13811..14944)
                     /gene="thiH"
                     /locus_tag="POB34_001706"
     CDS             complement(13811..14944)
                     /gene="thiH"
                     /locus_tag="POB34_001706"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDI5918675.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAVDVERALNASQFTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14941..15711)
                     /gene="thiG"
                     /locus_tag="POB34_001707"
     CDS             complement(14941..15711)
                     /gene="thiG"
                     /locus_tag="POB34_001707"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDI5918676.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETCAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15713..15913)
                     /gene="thiS"
                     /locus_tag="POB34_001708"
     CDS             complement(15713..15913)
                     /gene="thiS"
                     /locus_tag="POB34_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDI5918677.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15897..16652)
                     /gene="thiF"
                     /locus_tag="POB34_001709"
     CDS             complement(15897..16652)
                     /gene="thiF"
                     /locus_tag="POB34_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HDI5918678.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16645..17280)
                     /gene="thiE"
                     /locus_tag="POB34_001710"
     CDS             complement(16645..17280)
                     /gene="thiE"
                     /locus_tag="POB34_001710"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDI5918679.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17280..19175)
                     /gene="thiC"
                     /locus_tag="POB34_001711"
     CDS             complement(17280..19175)
                     /gene="thiC"
                     /locus_tag="POB34_001711"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDI5918680.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19256..19356)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19408..19884)
                     /gene="rsd"
                     /locus_tag="POB34_001712"
     CDS             complement(19408..19884)
                     /gene="rsd"
                     /locus_tag="POB34_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HDI5918681.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19979..20752
                     /gene="nudC"
                     /locus_tag="POB34_001713"
     CDS             19979..20752
                     /gene="nudC"
                     /locus_tag="POB34_001713"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HDI5918682.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20792..21856
                     /gene="hemE"
                     /locus_tag="POB34_001714"
     CDS             20792..21856
                     /gene="hemE"
                     /locus_tag="POB34_001714"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDI5918683.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLVRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21866..22537
                     /gene="nfi"
                     /locus_tag="POB34_001715"
     CDS             21866..22537
                     /gene="nfi"
                     /locus_tag="POB34_001715"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HDI5918684.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22580..23170
                     /locus_tag="POB34_001716"
     CDS             22580..23170
                     /locus_tag="POB34_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HDI5918685.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23357..23629
                     /gene="hupA"
                     /locus_tag="POB34_001717"
     CDS             23357..23629
                     /gene="hupA"
                     /locus_tag="POB34_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HDI5918686.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23642..24337
                     /locus_tag="POB34_001718"
     CDS             23642..24337
                     /locus_tag="POB34_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HDI5918687.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24339..24764)
                     /gene="zraP"
                     /locus_tag="POB34_001719"
     CDS             complement(24339..24764)
                     /gene="zraP"
                     /locus_tag="POB34_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HDI5918688.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            25002..26378
                     /gene="zraS"
                     /locus_tag="POB34_001720"
     CDS             25002..26378
                     /gene="zraS"
                     /locus_tag="POB34_001720"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HDI5918689.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26375..27700
                     /gene="zraR"
                     /locus_tag="POB34_001721"
     CDS             26375..27700
                     /gene="zraR"
                     /locus_tag="POB34_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HDI5918690.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDTLQATLEKALAHTHSIDAETPAVTASQFGMVGKSSAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGHFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27697..28986)
                     /gene="purD"
                     /locus_tag="POB34_001722"
     CDS             complement(27697..28986)
                     /gene="purD"
                     /locus_tag="POB34_001722"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDI5918691.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28998..30587)
                     /gene="purH"
                     /locus_tag="POB34_001723"
     CDS             complement(28998..30587)
                     /gene="purH"
                     /locus_tag="POB34_001723"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDI5918692.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7329 a         8053 c         8497 g         7175 t
ORIGIN      
        1 cgtccgcagt tctacttccg tactactgac gtgactggta ccatcgaact gccggaaggc
       61 gtagagatgg taatgccggg cgacaacatc aaaatggttg ttaccctgat ccacccgatc
      121 gcgatggacg acggtctgcg tttcgcaatc cgtgaaggcg gccgtaccgt tggcgcgggc
      181 gttgtagcaa aagttctgag ctaattgccg ataacatttg acgcaatgcg cactaaaagg
      241 gcatcatttg atgccctttt tgcacgcttt cgtaccagaa cctggctcat cagtgatttt
      301 ctttgtcata atcattgctg agacaggctc tgttgagggc gtataatccg aaaagctaat
      361 acgcgtttcg atttggtttg cctcgcgatc gcggggtgaa aatgtttgta gaaaacttct
      421 gacaggttgg tttatgagtg cgaataccga agctcaagga agcgggcgcg gcctggaagc
      481 gatgaagtgg gtcgttgtgg tggcattgct cctggtggcg attgtcggca actaccttta
      541 tcgcgacatt atgctgccgc tgcgtgcgct ggccgtagta attctgattg ctgcagcggg
      601 tggtgtcgcg ctgttaacga caaaaggtaa agctaccgtt gcttttgccc gtgaagcgcg
      661 taccgaagtc cgtaaggtca tttggccgac tcgccaggaa acattgcaca ccacgctgat
      721 tgtggctgcg gttaccgcag taatgtcact gatcctgtgg ggactggatg gtattctggt
      781 tcgcctggta tcctttatca ctggcctgag gttctgagat gtctgaagct cctaaaaagc
      841 gctggtacgt cgttcaggcg ttttccggtt ttgaaggccg cgtagcaacg tcgctgcgtg
      901 agcatatcaa attacacaac atggaagatt tgtttggtga agtcatggta ccaaccgaag
      961 aagtggttga aatccgtggc ggtcagcgtc gcaaaagcga acgtaaattc ttccctggct
     1021 acgtcctcgt tcagatggtg atgaacgacg cgagctggca cctggtgcgc agcgtaccgc
     1081 gtgtgatggg cttcatcggc ggtacttccg atcgtcctgc gccaatcagc gataaagaag
     1141 tcgatgcgat tatgaaccgc ctgcagcagg ttggtgataa gccgcgtccg aaaacgctgt
     1201 ttgaaccggg tgaaatggtc cgtgttaatg atggtccgtt cgctgacttc aacggtgttg
     1261 ttgaagaagt ggattacgag aaatctcgtc tgaaagtgtc tgtttctatc ttcggtcgtg
     1321 cgaccccggt agagctggac ttcagccagg ttgaaaaagc ctaacccagc gatcaaaaaa
     1381 gcggcgattt aatcgttgca caaggcgtga gattggaata caatttcgcg ccttttgttt
     1441 ttatgggcct tgcccgtaaa acgatttttt atatcacggg gagcctctca gaggcgttat
     1501 tacccaactt gaggaattta taatggctaa gaaagtacaa gcctatgtca agctgcaggt
     1561 tgcagctggt atggctaacc cgagtccgcc agtaggtccg gctctgggtc agcagggcgt
     1621 aaacatcatg gaattctgca aagcgttcaa cgcaaaaact gattccatcg aaaaaggtct
     1681 gccgattccg gtagtaatca ccgtttacgc tgaccgttct ttcactttcg ttaccaagac
     1741 tccgccggca gcagttctgc tgaaaaaagc ggctggtatc aagtctggtt ccggtaagcc
     1801 gaacaaagac aaagtgggta aaatttcccg cgctcagctg caggaaatcg cgcagaccaa
     1861 agctgccgac atgactggtg ccgacattga agcgatgact cgctccatcg aaggtactgc
     1921 acgttccatg ggcctggtag tggaggacta agaaatggct aaactgacca agcgcatgcg
     1981 tgttatccgc gagaaagttg atgcaaccaa acagtacgac atcaacgaag ctatcgcact
     2041 gctgaaagag ctggcgactg ctaaattcgt agaaagcgtg gacgtagctg ttaacctcgg
     2101 catcgacgct cgtaaatctg accagaacgt acgtggtgca actgtactgc cgcacggtac
     2161 tggccgttcc gttcgcgtag ccgtatttac ccaaggtgca aacgctgaag ctgctaaagc
     2221 tgcaggcgca gaactggtag gtatggaaga tctggctgac cagatcaaga aaggcgaaat
     2281 gaactttgac gttgttattg cttctccgga tgcaatgcgc gttgttggcc agctgggcca
     2341 ggttctgggt ccgcgcggcc tgatgccaaa cccgaaagtg ggtactgtaa caccgaacgt
     2401 tgctgaagcg gttaaaaacg ctaaagctgg ccaggttcgt taccgtaacg acaaaaacgg
     2461 catcatccac accaccatcg gtaaagtgga ctttgacgct gacaaactga aagaaaacct
     2521 ggaagctctg ctggttgcgc tgaaaaaagc aaaaccgact caggcgaaag gcgtgtacat
     2581 caagaaagtt agcatctcca ccaccatggg tgcaggtgtt gcagttgacc aggctggcct
     2641 gagcgcttct gtaaactaat gcctttacgt gggcggtgat tttgtctaca atcttacccc
     2701 cacgtataat gcttaatgca gacgtatatc cgagatattc gggttgtggc aaggcggcaa
     2761 ctgagtgagt cgccaggagc atagctaact atgtgactgg tgcgaatgaa ggaagccaac
     2821 gccgccacaa gttgaatagc gacggataga aaagatttgt tcgttggagc ctggcctatc
     2881 caggcctccg tcgaagaccg caggagtttc gcaagaaact taatcccctg cgtagacggt
     2941 gacagaacgc taagattatt cttttatatt ctggcttgtt tctgctcacc gtaattaaga
     3001 cgctctctcc gtttggagga gtgaagtgag ttccagagat tttctctggc aaacatccag
     3061 gagcaaagct aatggcttta aatcttcaag acaaacaagc gattgttgct gaagtcagcg
     3121 aagtagccaa aggcgcgctg tctgcagtag ttgcggattc ccgtggcgta actgtagata
     3181 aaatgactga actgcgtaaa gcaggtcgcg aagctggcgt atacatgcgt gttgttcgta
     3241 acaccctgct gcgccgtgct gttgaaggta ctccgttcga gtgcctgaaa gacgcgtttg
     3301 ttggtccgac cctgattgca tactctatgg aacacccggg cgctgctgct cgtctgttca
     3361 aagagttcgc gaaagcgaat gcaaaatttg aggtcaaagc cgctgccttt gaaggtgagc
     3421 tgatcccggc gtctcagatc gaccgcctgg caactctgcc gacctacgaa gaagcaattg
     3481 cacgcctgat ggcaaccatg aaagaagctt cggctggcaa actggttcgt actctggctg
     3541 ctgtacgcga tgcgaaagaa gctgcttaat cgcagttatc tttttaacgc attcgcttac
     3601 gtataaactt attctgatat tcaggaacaa tttaaatgtc tatcactaaa gatcaaatca
     3661 ttgaagcagt tgcagctatg tctgtaatgg acgttgtaga actgatctct gcaatggaag
     3721 aaaaattcgg tgtttccgct gctgctgctg tagctgtagc tgctggcccg gttgaagctg
     3781 ctgaagaaaa aactgaattc gacgtaattc tgaaagctgc tggcgctaac aaagttgctg
     3841 ttatcaaagc agtacgtggc gcaactggcc tgggtctgaa agaagctaaa gacctggtag
     3901 aatctgcacc ggctgctctg aaagaaggcg tgagcaaaga cgacgcagaa gcactgaaaa
     3961 aagctctgga agaagctggc gctgaagttg aagttaaata agccaaccct tccggttgca
     4021 gcctgagaaa tcaggctgat ggctggtgac tttttagtca ccagcctttt tgcgctgtaa
     4081 ggcgccagta gcgtttcaca ctgtttgact actgctgtgc ctttcaatgc ttgtttctat
     4141 cgacgactta atatactgcg acaggacgtc cgttctgtgt aaatcgcaat gaaatggttt
     4201 aagcgtgata gcaacaggca ttgcggaaag tgttccattt tccggtcaac aaaatagtgt
     4261 tgcacaaact gtccgctcaa tggacagatg ggtcgacttg tcagcgagct gaggaaccct
     4321 atggtttact cctataccga gaaaaaacgt attcgtaagg attttggtaa acgtccacaa
     4381 gttctggatg taccttatct cctttctatc cagcttgact cgtttcagaa atttatcgag
     4441 caagatcctg aagggcagta tggtctggaa gctgctttcc gttccgtatt cccgattcag
     4501 agctacagcg gtaattccga gctgcaatac gtcagctacc gccttggcga accggtgttt
     4561 gacgtccagg aatgtcaaat ccgtggcgtg acctattccg caccgctgcg cgttaaactg
     4621 cgtctggtga tctatgagcg cgaagcgccg gaaggcaccg taaaagacat taaagaacaa
     4681 gaagtctaca tgggcgaaat tccgctcatg acagacaacg gtacctttgt tatcaacggt
     4741 actgagcgtg ttatcgtttc ccagctgcac cgtagtccgg gcgtcttctt tgactccgac
     4801 aaaggtaaaa cccactcttc gggtaaagtg ctgtataacg cgcgtatcat cccttaccgt
     4861 ggttcctggc tggacttcga attcgatccg aaggacaacc tgttcgtacg tatcgaccgt
     4921 cgccgtaaac tgcctgcgac catcattctg cgcgccctga actacaccac agagcagatc
     4981 ctcgacctgt tctttgaaaa agttatcttt gaaatccgtg ataacaagct gcagatggaa
     5041 ctggtgccgg aacgcctgcg tggtgaaacc gcatcttttg acatcgaagc taacggtaaa
     5101 gtgtacgtag aaaaaggccg ccgtatcact gcgcgccaca ttcgccagct ggaaaaagac
     5161 gacgtcaaac tgatcgaagt cccggttgag tacatcgcag gtaaagtggt tgctaaagac
     5221 tatattgatg agtctaccgg cgagctgatc tgcgcagcga acatggagct gagcctggat
     5281 ctgctggcta agctgagcca gtctggtcac aagcgtatcg aaacgctgtt caccaacgat
     5341 ctggatcacg gcccatatat ctctgaaacc ttacgtgtcg acccaactaa cgaccgtctg
     5401 agcgcactgg tagaaatcta ccgcatgatg cgccctggcg agccgccgac tcgtgaagca
     5461 gctgaaagcc tgttcgagaa cctgttcttc tccgaagacc gttatgactt gtctgcggtt
     5521 ggtcgtatga agttcaaccg ttctctgctg cgcgaagaaa tcgaaggttc cggtatcctg
     5581 agcaaagacg acatcattga tgttatgaaa aagctcatcg atatccgtaa cggtaaaggc
     5641 gaagtcgatg atatcgacca cctcggcaac cgtcgtatcc gttccgttgg cgaaatggcg
     5701 gaaaaccagt tccgcgttgg cctggtacgt gtagagcgtg cggtgaaaga gcgtctgtct
     5761 ctgggcgatc tggataccct gatgcctcag gatatgatca acgccaagcc gatttccgca
     5821 gcagtgaaag agttcttcgg ttccagccag ctgtctcagt ttatggacca gaacaacccg
     5881 ctgtctgaga ttacgcacaa acgtcgtatc tccgcactcg gcccaggcgg tctgacccgt
     5941 gaacgtgcag gcttcgaagt tcgagacgta cacccgactc actacggtcg cgtatgtcca
     6001 atcgaaaccc ctgaaggtcc gaacatcggt ctgatcaact ctctgtccgt gtacgcacag
     6061 actaacgaat acggcttcct tgagactccg tatcgtaaag tgaccgacgg tgttgtaact
     6121 gacgaaattc actacctgtc tgctatcgaa gaaggcaact acgttatcgc ccaggcgaac
     6181 tccaacctgg atgaagaagg ccacttcgta gaagacctgg taacttgccg tagcaaaggc
     6241 gaatccagct tgttcagccg cgaccaggtt gactacatgg acgtatccac ccagcaggtg
     6301 gtatccgtcg gtgcgtccct gatcccgttc ctggaacacg atgacgccaa ccgtgcattg
     6361 atgggtgcga acatgcaacg tcaggccgtt ccgactctgc gcgctgataa gccgctggtt
     6421 ggtactggta tggaacgtgc tgttgccgtt gactccggtg taactgcggt agctaaacgt
     6481 ggtggtgtcg ttcagtacgt ggatgcttcc cgtatcgtta tcaaagttaa cgaagacgag
     6541 atgtatccgg gtgaagcagg tatcgacatc tacaacctga ccaaatacac ccgttctaac
     6601 cagaacacct gtatcaacca gatgccgtgt gtgtctctgg gtgaaccggt tgaacgtggc
     6661 gacgtgctgg cagacggtcc gtccaccgac ctcggtgaac tggcgcttgg tcagaacatg
     6721 cgcgtagcgt tcatgccgtg gaatggttac aacttcgaag actccatcct cgtatccgag
     6781 cgtgttgttc aggaagaccg tttcaccacc atccacattc aggaactggc gtgtgtgtcc
     6841 cgtgacacca agctggggcc ggaagagatc accgctgaca tcccgaacgt gggtgaagct
     6901 gcgctctcca aactggatga atccggtatc gtttacattg gtgcggaagt gaccggtggc
     6961 gacattctgg ttggtaaggt aacgccgaaa ggtgaaactc agctgacccc agaagaaaaa
     7021 ctgctgcgtg cgatcttcgg tgagaaagcc tctgacgtta aagactcttc tctgcgcgta
     7081 ccaaacggtg tatccggtac ggttatcgac gttcaggtct ttactcgcga tggcgtagaa
     7141 aaagacaaac gtgcgctgga aatcgaagaa atgcagctca aacaggcgaa gaaagacctg
     7201 tctgaagaac tgcagatcct cgaagcgggt ctgttcagcc gtatccgtgc tgtgctggta
     7261 gccggtggcg ttgaagctga gaagctcgac aaactgccgc gcgatcgctg gctggagctg
     7321 ggcctgacag acgaagagaa acaaaatcag ctggaacagc tggctgagca gtatgacgaa
     7381 ctgaaacacg agttcgagaa gaaactcgaa gcgaaacgcc gcaaaatcac ccagggcgac
     7441 gatctggcac cgggcgtgct gaagattgtt aaggtatatc tggcggttaa acgccgtatc
     7501 cagcctggtg acaagatggc aggtcgtcac ggtaacaagg gtgtaatttc taagatcaac
     7561 ccgatcgaag atatgcctta cgatgaaaac ggtacgccgg tagacatcgt actgaacccg
     7621 ctgggcgtac cgtctcgtat gaacatcggt cagatcctcg aaacccacct gggtatggct
     7681 gcgaaaggta tcggcgacaa gatcaacgcc atgctgaaac agcagcaaga agtcgcgaaa
     7741 ctgcgcgaat tcatccagcg tgcgtacgat ctgggcgctg acgttcgtca gaaagttgac
     7801 ctgagtacct tcagcgatga agaagttatg cgtctggctg aaaacctgcg caaaggtatg
     7861 ccaatcgcaa cgccggtgtt cgacggtgcg aaagaagcag aaattaaaga gctgctgaaa
     7921 cttggcgacc tgccgacttc cggtcagatc cgcctgtacg atggtcgcac tggtgaacag
     7981 ttcgagcgtc cggtaaccgt tggttacatg tacatgctga aactgaacca cctggtcgac
     8041 gacaagatgc acgcgcgttc caccggttct tacagcctgg ttactcagca gccgctgggt
     8101 ggtaaggcac agttcggtgg tcagcgtttc ggggagatgg aagtgtgggc gctggaagca
     8161 tacggcgcag catacaccct gcaggaaatg ctcaccgtta agtctgatga cgtgaacggt
     8221 cgtaccaaga tgtataaaaa catcgtggac ggcaaccatc agatggagcc gggcatgcca
     8281 gaatccttca acgtattgtt gaaagagatt cgttcgctgg gtatcaacat cgaactggaa
     8341 gacgagtaat tctcgctcaa acaggtcact gctgtcgggt taaaacccgg cagcggattg
     8401 tgctaactcc gacgggagca aatccgtgaa agatttatta aagtttctga aagcgcagac
     8461 taaaaccgaa gagtttgatg cgatcaaaat tgctctggct tcgccagaca tgatccgttc
     8521 atggtctttc ggtgaagtta aaaagccgga aaccatcaac taccgtacgt tcaaaccaga
     8581 acgtgacggc cttttctgcg cccgtatctt tgggccggta aaagattacg agtgcctgtg
     8641 cggtaagtac aagcgcctga aacaccgtgg cgtcatctgt gagaagtgcg gcgttgaagt
     8701 gacccagact aaagtacgcc gtgagcgtat gggccacatc gaactggctt ccccgactgc
     8761 gcacatctgg ttcctgaaat cgctgccgtc ccgtatcggt ctgctgctcg atatgccgct
     8821 gcgcgatatc gaacgcgtac tgtactttga atcctatgtg gttatcgaag gcggtatgac
     8881 caacctggaa cgtcagcaga tcctgactga agagcagtat ctggacgcgc tggaagagtt
     8941 cggtgacgaa ttcgacgcga agatgggggc ggaagcaatc caggctctgc tgaagagcat
     9001 ggatctggag caagagtgcg aacagctgcg tgaagagctg aacgaaacca actccgaaac
     9061 caagcgtaaa aagctgacca agcgtatcaa actgctggaa gcgttcgttc agtctggtaa
     9121 caaaccagag tggatgatcc tgaccgttct gccggtactg ccgccagatc tgcgtccgct
     9181 ggttccgctg gatggtggtc gtttcgcgac ttctgacctg aacgatctgt atcgtcgcgt
     9241 cattaaccgt aacaaccgtc tgaaacgtct gctggatctg gctgcgccgg acatcatcgt
     9301 acgtaacgaa aaacgtatgc tgcaggaagc ggtagacgcc ctgctggata acggtcgtcg
     9361 cggtcgtgcg atcaccggtt ctaacaagcg tcctctgaaa tctttggccg acatgatcaa
     9421 aggtaaacag gggcgtttcc gtcagaacct gctcggtaag cgtgttgact actccggtcg
     9481 ttctgtaatc accgtaggtc catacctgcg tctgcatcag tgcggtctgc cgaagaaaat
     9541 ggcgctggag ctgttcaaac cgttcatcta cggcaagctg gaactgcgtg gtcttgctac
     9601 caccattaaa gctgcgaaga aaatggttga gcgcgaagaa gctgtcgttt gggatatcct
     9661 ggacgaagtt atccgcgaac acccggtact gctgaaccgt gcaccgactc tgcaccgtct
     9721 gggtatccag gcatttgaac cggtactgat cgaaggtaaa gctatccagc tgcacccgct
     9781 ggtttgtgcg gcatataacg ccgacttcga tggtgaccag atggctgttc acgtaccgct
     9841 gacgctggaa gcccagctgg aagcgcgtgc gctgatgatg tctaccaaca acatcctgtc
     9901 cccggcgaac ggcgaaccaa tcatcgttcc gtctcaggac gttgtactgg gtctgtacta
     9961 catgacccgt gactgtgtta acgccaaagg cgaaggcatg gtgctgactg gcccgaaaga
    10021 agcagaacgt ctgtatcgct ctggtctggc ttctctgcat gcgcgcgtta aagtgcgtat
    10081 caccgagtat gaaaaagatg ctaacggtga attagtagcg aaaaccagcc tgaaagacac
    10141 gactgttggc cgtgccattc tgtggatgat tgtaccgaaa ggtctgcctt actccatcgt
    10201 caaccaggcg ctgggtaaaa aagcaatctc caaaatgctg aacacctgct accgcattct
    10261 cggtctgaaa ccgaccgtta tttttgcgga ccagatcatg tacaccggct tcgcctatgc
    10321 agcgcgttct ggtgcatctg ttggtatcga tgacatggtc atcccggaga agaaacacga
    10381 aatcatctcc gaggcagaag cagaagttgc tgaaattcag gagcagttcc agtctggtct
    10441 ggtaactgcg ggcgaacgct acaacaaagt tatcgatatc tgggctgcgg cgaacgatcg
    10501 tgtatccaaa gcgatgatgg ataacctgca aactgaaacc gtgattaacc gtgacggtca
    10561 ggaagagaag caggtttcct tcaacagcat ctacatgatg gccgactccg gtgcgcgtgg
    10621 ttctgcggca cagattcgtc agcttgctgg tatgcgtggt ctgatggcga agccggatgg
    10681 ctccatcatc gaaacgccaa tcaccgcgaa cttccgtgaa ggtctgaacg tactccagta
    10741 cttcatctcc acccacggtg ctcgtaaagg tctggcggat accgcactga aaactgcgaa
    10801 ctccggttac ctgactcgtc gtctggttga cgtggcgcag gacctggtgg ttaccgaaga
    10861 cgattgtggt acccatgaag gtatcatgat gactccggtt atcgagggtg gtgacgttaa
    10921 agagccgctg cgcgatcgcg tactgggtcg tgtaactgct gaagacgttc tgaagccggg
    10981 tactgctgat atcctcgttc cgcgcaacac gctgctgcac gaacagtggt gtgacctgct
    11041 ggaagagaac tctgtcgacg cggttaaagt acgttctgtt gtatcttgtg acaccgactt
    11101 tggtgtatgt gcgcactgct acggtcgtga cctggcgcgt ggccacatca tcaacaaggg
    11161 tgaagcaatc ggtgttatcg cggcacagtc catcggtgaa ccgggtacac agctgaccat
    11221 gcgtacgttc cacatcggtg gtgcggcatc tcgtgcggct gctgaatcca gcatccaggt
    11281 gaaaaacaaa ggtagcatca agctcagcaa cgtgaagtcg gttgtgaact ccagcggtaa
    11341 actggttatc acttcccgta atactgaact gaaactgatc gacgaattcg gtcgtaccaa
    11401 agaaagctac aaagtgcctt acggtgcggt actggcgaaa ggcgatggcg aacaggttgc
    11461 tggcggcgaa accgttgcaa actgggaccc gcacaccatg ccggttatca ccgaagtaag
    11521 cggttttgta cgctttactg acatgatcga cggtcagacc attactcgtc agacagacga
    11581 attgaccggt ctgtcttcgc tggtggttct ggattccgca gaacgtaccg caggtggtaa
    11641 agatctgcgt ccggcactga aaatcgttga tgctcagggt aacgacgttc tgatcccagg
    11701 taccgatatg cctgcgcagt acttcctgcc gggtaaagcg attgttcagc tggaagatgg
    11761 cgtacagatc agctctggtg acaccctggc gcgtattccg caggaatccg gcggtaccaa
    11821 ggacatcacc ggtggtctgc cgcgcgttgc ggacctgttc gaagcacgtc gtccgaaaga
    11881 gccggcaatc ctggctgaaa tcagcggtat cgtttccttc ggtaaagaaa ccaaaggtaa
    11941 acgtcgtctg gttatcaccc cggtagacgg tagcgatccg tacgaagaga tgattccgaa
    12001 atggcgtcag ctcaacgtgt tcgaaggtga acgtgtagaa cgtggtgacg taatttccga
    12061 cggtccggaa gcgccgcacg acattctgcg tctgcgtggt gttcatgctg tgactcgtta
    12121 catcgttaac gaagtacagg acgtataccg tctgcagggc gttaagatta acgataaaca
    12181 catcgaagtt atcgttcgtc agatgctgcg taaagctacc atcgttaacg cgggcagctc
    12241 cgacttcctg gaaggcgaac aggttgaata ctctcgcgtc aagatcgcaa accgcgaact
    12301 ggaagcgaac ggcaaagtgg gggcaactta ctcccgcgat ctgctgggta tcaccaaagc
    12361 gtctctggca accgagtcct tcatctccgc ggcatcgttc caggagacca ctcgcgtgct
    12421 gaccgaagca gccgttgcgg gcaaacgcga cgaactgcgc ggcctgaaag agaacgttat
    12481 cgtgggtcgt ctgatcccgg caggtaccgg ttacgcgtac caccaggatc gtatgcgtcg
    12541 ccgtgctgcg ggtgaagctc cggctgcacc gcaggtgact gcagaagacg catctgccag
    12601 cctggcagaa ctgctgaacg caggtctggg cggttctgat aacgagtaat cgttaatccg
    12661 caaataacgt aaaaacccgc ttcggcgggt ttttttatgg ggggagttta gggaaagagc
    12721 atttgtcaga atatttaagg aatttctgaa tactcataat caatgtagag atgactaata
    12781 tcctgaaact gactgaacta attgagtcaa actcggcaag gattcgatac tattcctgtg
    12841 taactttctt aaggaacgag aatgaaacag gaagtggaaa agtggcgacc ttttggacat
    12901 ccggatggtg atattcgtga tttatcattt cttgatgctc atcaggctgt ctacgttcag
    12961 catcatgagg gcaaagagcc tttagagtat cgcttttggg ttacctactc tcttcactgc
    13021 ttcacaaaag attatgaaca tcagacgaac gaagaaaaac aatcgttaat gtaccacgcg
    13081 cctaaagaat ctcgtccctt ctgccagcac cgttataact tagcgcgcac acacttaaaa
    13141 agaactattt tggcgctgcc agaaagcaac gttattcatg ccgggtatgg tagctatgcc
    13201 gtgattgagg tggacttaga cggaggagat aaggcatttt actttgttgc gttcagggct
    13261 ttcagggaaa agaaaaaact ccgtttgcat gtaactagcg cttatcccat ttctgaaaaa
    13321 cagaaaggta aatcagtgaa atttttcacc attgcctaca acttattgag aaataagcag
    13381 cttcctcagc cctcaaaata acaaaaccca ccttaaggtg ggtttcgcca gagaattatc
    13441 tctggtattc agaacgccat taccggactt tgccttgacc ttgcgataat cgcaggttgc
    13501 gggatgtctg aatttcttca gtctgctgca tcctggaaga tgagaacatg tgttcttatt
    13561 ttcgtctcta tcatagttga gtatttactc tcttacaatc agatctcttt cattgctcaa
    13621 caggcgatgg cttcagactt tgcattacgg aatttttaag aaaggcaggg cgaaacgagg
    13681 aagaagcttt tcgccctgga caacattcct gctgacggca ctaccataaa aaaacagaac
    13741 gctttcaaca accttgcgtt tttcatcttc cgtagcggct cggaataaat ttacgttctt
    13801 acattccgtc tcatagtctt tgcgaggccc gtcccagata gctgtcccag tctttccata
    13861 ccggctgcaa accctgagcg gttaacgcgg cagcaaccgc ttccggtctg cgatcgtcgt
    13921 gcggtgagaa ctgttccaac tcggggtgat tatcggcata gccacctggc tgcgttttcg
    13981 agaaggcgct gacgttattg atcgccagcg gaataacgcg atcgcgaaac cacggtgatt
    14041 cccgcgtgga gagtgacagt tcaatctccg gtgcaagcag tcggaaggcg cagatggttt
    14101 gcactaactg gcgttcatcc ataatcgacg caggctcaat gccgccagta cacgggcgca
    14161 ggcgcggaaa ggagacagag taacggcttt gccagtaatg ctgttgcagc catagcaaat
    14221 gttcggcaac catatagcag tcaacgcgcc agttgtcgga aaggccaatt agcgcgccga
    14281 ggcctatctt atcaatcccc gcacgcccca gccgatccgg cgtttccagc cgccagaaga
    14341 agtcctgttt tttgcctttc agatgatggc gggcataagt cgcctcgtga tatgtctcct
    14401 gataaaccat cacgccatcc agaccaagct gctttaactc ggcgtattcc gtctccgcca
    14461 gcggttgcac ttccatttgt agtgaagaga actgttcacg cagggcgggg agatgacgac
    14521 gaaagtaatc catccccact ttcgcctgat gttcaccagt gactaatagc agatgttcaa
    14581 agcccatctc ccgtatagcg gcgctttccc tggcaatatc cgcttcatcc agcgttttgc
    14641 gcttgatgcg attactcatg gaaaatccgc agtacgtgca gtcgttagcg caaagattgg
    14701 aaagataaag cgggacgtag aaactaactg tgttgccaaa tcgctgacgg gtcagacgct
    14761 gcgcccgttg ggccagttgt tccagatagc cactggcggc tggcgataac agcgccatca
    14821 tatcgtcgcg ggtgaattgc gaggcattta gcgcccgctc tacgtcaaca gccgttttgc
    14881 cgttgatacg caggcggatg tcatcccagt ccagttgtcg ccagcgatcg ctgaaggttt
    14941 tcatgccgat gcctccagaa atccagtcag cgggctggtg gcatgagcaa aatgactgcg
    15001 gctgcccggt ccggactgac gtgccagcag gcctgcttct accgccagac gaaatgcctt
    15061 cgccatgttg acgggatcgt ccgctacggc aatcgccgta ttcactaaca ccgcgtcggc
    15121 ccccatttcc agggcctgcg cggcatggct gggaacgccg atgccagcat cgacaaccac
    15181 cggaactgtg gcctgctgga taataatctc cagcatggca caggtttcca gtccctgatt
    15241 cgagccaatc ggcgcgccga gcggcatcac cgctgcacag ccgacttctt ccagacgttt
    15301 acacaatacc ggatcggccc cgcagtaagg cagcacgaca aatccctgtt gtaccagcat
    15361 ttcggcggct ttcagagttt cgatgggatc gggcaacagc cagcgggcgt cagggtgaat
    15421 ctccaatttt agccagttag tgcctaacgc ttcacgtgcc agatgggcgg cgaaaatggc
    15481 ttcttccgct gttttcgccc cggatgtatt tggcagcagg gtcacacccg ccgcgataag
    15541 cggttcgagg atagcgtcgt tgtgctggcg caagtcgaca cgtttcatcg ccagtgtcac
    15601 cagctggctg ccggaagcgc ggatcgcctc caccatcagt tgtgaagaag cgaatttccc
    15661 tgtgccggta aacagatgtg aatcaaacgt tttgtccgca atacgtaaca tttcaacccc
    15721 ctgcaataac ctgaaaaagc aggatctggt cgccatcctg cacgatatgt tgcgcccact
    15781 gctcacgcgg gacgatttgc tgattaatcg ccagagccgc gcccgcttgt cgttggtcga
    15841 gttgctccag tagttcgtga acagtttgcc cggcggcgca ctgcatcggt tgatcgttaa
    15901 acaggatctg cattgcttcc tccgcatacc gggcaaccac tggcgcggcg caacgccagg
    15961 ctgcgccact ggctcgattt accgtcgaac agtcggagtt ctcccgcagg tgtctctata
    16021 ccgcttaata acttaatggc ttccagtgcc tgcaaagtgc ccataacccc gaccaccggg
    16081 ccaaccacgc ccgccgtgcg gcagttgcgt tctggctcct ggttatctgg ccacaggcag
    16141 cggtaacacc cctgctccca gggcggcgtc agtaccatca actgaccgcc aaatccgacc
    16201 gcgctggcgg tgataagcgg cgtgttgagt gccacgcagg cggcattaat ctcctggcga
    16261 gtcgccatat tgtcggtaca gtcgagcacc acatcggccc gtgcaaccgc atcttttaac
    16321 gcctcacccg ttaaccgttg ttgtaatgct gtcagttgaa tgtcgggatt caactgtgtc
    16381 agtcgctgtt ggctgacctg cgatttcggg cgatcgatat cttcagtggt aaagaggatt
    16441 tgtcgttgca gattgcttaa atgcacatcg tcgtcatctg ccagtaccag cgtcccgacg
    16501 ccagcgcccg ccaggtacag cgcagcaggt gtacccagcc cgcccagacc gataatcagc
    16561 acctggctgt cgagcagttt ttgctgcccg tccagagcga tatcgtcgag caggatttgg
    16621 cggctataac gcataaagtc acggtcattc atcgccaact cctgcaattt ccagcaactg
    16681 tgccgttgcc aaacgccagt ctgcagcttg agtaatggcg ctgacgacgg cgatactgcc
    16741 gacacccgtt gctatcaccg caggcgcgcg tgccagactg ataccgccaa tcgccacggt
    16801 gggataatcc gccagtcgct caacatgccg tgccagctgt tccagcccct gcggtgcaga
    16861 aggcatctgt ttggtttgcg tcgggaacac atgtcccagc gcgatataag aggggcgtgc
    16921 tgccagcgcg acgtcgattt ccatatcgtc atgtgtcgaa acgcccagcc gcaggcctgc
    16981 cgcgcggatg gcgttgagat cggtggcttg caaatcttcc tgccccaaat ggacgccata
    17041 cgcctgatgc ttgatcgcca gccgccagta atcgttgata aacaatcgcg cgttatagcg
    17101 gcggcccagc gcaattgccg ccacgacatc ggcttccacc tcttcatcgc gccgatcttt
    17161 gatgcgtagc tggagagtac gtacgcctgc atccaacaga cgttcgatcc actgtacgct
    17221 gtccaccacc gggtacagtc ctaaacgaaa aggtacagga ggaaaatcag gctgatacat
    17281 cacgcttcct ccttacgcag gtagatttct ccgcctctgg cacggaagtt ctccgacata
    17341 tccgccattc ccatttcaat agtttgcgtg gcggcgtaat cacgcacttc ctggctgatt
    17401 ttcatcgagc aaaatttcgg tccacacatg gagcaaaaat gggcgacttt gccggactct
    17461 tggggcaggg tttcatcgtg ataagcgcgg gcggtaaacg ggtcgagggc cagattaaac
    17521 tggtcttccc agcgaaattc gaagcgggct ttcgacatgg cgttatcgcg aatttgcgcg
    17581 cccggatgcc ctttcgccag gtcagcggcg tgggcggcaa tcttataggt gataagcccc
    17641 tgcttaacat cttctttatt aggcagaccc agatgctctt ttggcgttac gtaacagagc
    17701 atcgcgcagc caaaccagcc aatcatcgcc gcaccaatcc ccgacgtgaa gtggtcatag
    17761 cccggcgcaa tatcggtagt tagcggcccc agagtgtaaa acggcgcttc gtggcagtgc
    17821 tctaactcct cggtcatatt gcggcggatc atctgcatcg gcacgtggcc tgggccttca
    17881 atcatcacct gcacgtcata ttcccaggca attttggtca gttcgcccag cgtatgcagc
    17941 tcggcaaact gcgcttcatc gttggcgtcc tgaatagaac cggggcgcag accgtcgccc
    18001 agcgacagcg aaacgtcata agcggcacag atttcacaaa tttcgcggaa gtgttgatag
    18061 aggaaatttt cctgatgatg ggagaggcac catttcgcca taatcgaacc gccgcgagag
    18121 acgataccgg tcaggcgttt cgcggtcatc ggcacatagc gcagcagtac gcccgcatgg
    18181 atagtgaagt aatccacacc ttgctcggcc tgttccagca gcgtgtcgcg gaacgcttcc
    18241 caggtaagat cttcggcgat cccgttaacc ttctccagcg cctggtagat cggcactgta
    18301 ccgatcggca ccgggctgtt acgcaaaatc cactcgcggg tttcgtgaat atagcgaccg
    18361 gtggagagat ccatcaccgt atccgctccc cagcgcgtgg accataccag cttttccact
    18421 tcttcttcga tggaagaggt gaccgccgag ttgccgatat tggcgttaac ttttaccagg
    18481 aaattgcgac caataatcat cggctccgat tccggatgat taatgttggc cgggataatc
    18541 gcacgtccgg cagcaacttc atcacggaca aattccgcag tgatattttc cggcagatgt
    18601 gcgccaaagc tcattcccgg atgctggtgg cgtaaaacct cgctacggat gcgctcgcgg
    18661 cccatattct cgcggatggc gatgaattcc atttccggcg tgatgatgcc ctggcgggcg
    18721 tagtgcagtt gggtgacacg gcgtcctgct ttggcgcgtt ttggtgttag tacgccgcta
    18781 aaacgcagtt cgtcgaggcc atcatctgcc agccgcgctt tagtgtaatc ggaactgcgc
    18841 acggtaagtt cttcggtatc gccgcgcgca tcgatccacg gctggcgtag ttttgccagc
    18901 ccttgctgca cgttaatggc aatctgcgga tcaccatacg ggccggaggt gtcgtagacc
    18961 ggaatcgctt cgttttcttc gtactgcggc tgttctttgc taccgccaat tagcgtcggg
    19021 ctaagctgga tctcacgcat cggcacgcgc acgccggggt gtgtgccagt gatataaatg
    19081 cgttttgagt tgggaaaggc ggtgccttcc agggtgtcga taaaatgttg ggcccgggcg
    19141 cgttgttcgc ggcgggtcag ttttgttgca gacatagctc attccaaaaa gttaaggacg
    19201 tggcttgtca gacgacggat gaagcaagag acgatcgccg caggggcgat gcgatagcag
    19261 attaactctt gttcccttcg caggtattag cctgatcagg ttccgcggat cccgaataaa
    19321 cggtctcagc cagttaaggc actccgacaa gaaattagcc ccgcaaatgg ggcattgaat
    19381 gtaaattacg cgttaacagc gcagaactca agcaggatgt ttgacgcggg cggcgtcaag
    19441 caccagcaga atcagcttat cttccagcac aaagcgcgct tccagcgcct cgccgatgtc
    19501 agataaaacc tgttgaaact caaggtaatt atcatgatcg atggcggttt ccagactgga
    19561 atcgtagtaa tccataatct gttgagtatt ggcttcgagt tgcggccaaa tcttcgcggc
    19621 gcgcgcgagt tgcccgttgc cttccagctt atgaagaatg cgctcataaa tactgaaatg
    19681 tccggcagac aagtaatcga ccaggctctg acaaaaatca tcaagggctt tttcgtttag
    19741 cctcatgtac gattctttgc caggcttaat gccaaccaga ttgtagtaag ccacgagcag
    19801 atgcttacgt acatgtagcc agcgatcaac cagtttgtta cttcctctga cgcgctccgt
    19861 caggttatcg agctggttaa gcatgattga ctccgcaagt ttgtattcaa aaactgctca
    19921 gtgagaaatg taaaaaccat gttaaacatg ccagtgatgc aaaggtagtg caagagctat
    19981 ggatcgtata attgaaaaat tagatcacgg ctggtgggtc gtcagccatg aacaaaaatt
    20041 atggttgccg aagggagaat tgccatatgg cgaagcggca aatttcgatc ttgtgggtca
    20101 gcgcgcacta caaatcggcg aatggcaggg ggaacctgtt tggttagtac aacagcagcg
    20161 gcgtcacgat atggggtcgg tacgtcaggt cattgatctc gatgttgggc tgtttcaact
    20221 ggccggacga ggcgtacaac tggcggagtt ttaccgatcg cataaatact gtggttactg
    20281 cgggcatgaa atgtatccga gcaaaaccga atgggcgatg ctgtgcagcc attgccgtga
    20341 gcgttactac ccgcaaatcg ccccctgcat tattgttgcc atccgtcgcg atgattcgat
    20401 cctcctcgcc cagcataccc gccatcgtaa cggtgtccat acagtacttg ccggattcgt
    20461 cgaagtgggc gaaaccctcg agcaggcagt cgcgcgggaa gtgatggaag agagcggaat
    20521 taaagttaaa aacttgcgtt acgtgacttc tcagccgtgg ccgtttcctc agtctttaat
    20581 gaccgcgttt atggcggaat atgacagcgg cgacatcgtg atcgacccga aagaattgct
    20641 cgaggcgaac tggtatcgct atgacgattt gccgttactc ccgccgcccg gcaccgtagc
    20701 gcgccgtctg atagaagata cggtggcgat gtgtcgggca gagtatgagt gatgatacac
    20761 tgaccgcctg acgcactaag gaacagccaa aatgaccgaa cttaaaaacg atcgttatct
    20821 gcgggcgctg ctgcgccagc ccgttgatgt cactccagta tggatgatgc gccaggcggg
    20881 tcgctatcta ccggaatata aagccacgcg cgcccaggcg ggcgatttta tgtcgctgtg
    20941 caaaaacgcc gagctggcgt gcgaagtgac tttgcaaccg ctgcgtcgct acccgctgga
    21001 tgcggcgatc ctcttttccg atatcctcac cgtgccggac gcgatggggt tagggctcta
    21061 ttttgaagcc ggagaaggtc cgcgttttac ctcgccagtc acctgcaaag ccgacgtcga
    21121 taaactgcca attccggacc cggaagatga actgggttac gtgatgaacg cggtgcgtac
    21181 cattcgtcgc gaactgaaag gcgaagtgcc gctgattggt ttttccggca gcccgtggac
    21241 gctggcgacc tacatggtgg aaggcggcag cagcaaagcc ttcaccgtga tcaaaaaaat
    21301 gatgtatgcc gatccgcagg cgctgcacgc tctgctcgat aaactggcga aaagcgtcac
    21361 tttgtatctg aatgcgcaga ttaaagccgg tgctcaggca gtgatgattt tcgacacctg
    21421 gggcggcgtg cttaccgggc gcgattatca acagttctcg ctctattaca tgcataaaat
    21481 tgttgatggt ttagtgcgtg aaaacgacgg tcgccgcgta ccggtcacgc tgtttaccaa
    21541 aggcggcgga cagtggctgg aagccatggc agaaaccggt tgcgatgcgc tgggcctcga
    21601 ctggacaacg gacatcgccg atgcgcgccg ccgtgtgggc aataaagtcg cgttgcaggg
    21661 taatatggat ccgtcgatgc tgtacgcgcc gcctgcccgc attgaagaag aagtagcgac
    21721 tatacttgca ggtttcggtc acggcgaagg tcatgtcttt aaccttggtc acggcattca
    21781 tcaggatgtg ccgccagaac atgctggcgt gttcgtggag gcagtgcatc gactgtctga
    21841 acagtatcac cgctaaggag tgattatgga tctcgcgtca ttacgcgctc aacaaatcga
    21901 actggcttct tctgtgatcc gcgaggatcg actcgataaa gatccaccgg atctgatcgc
    21961 cggagccgat gtcgggtttg agcagggcgg agaagtgacg cgagcggcga tggtgctgct
    22021 gaaatatccc tcgcttgagc tggtggagta taaagttgcc cgcatcgcca ccaccatgcc
    22081 ttacattcca ggttttcttt ccttccgcga atatcctgcg ctgctggcag cgtgggagat
    22141 gctgtcgcaa aagccggatt tagtgtttgt cgatggtcat gggatctcgc atcctcgccg
    22201 tcttggcgtc gccagccatt ttggcttatt ggtggatgtg ccgaccattg gcgtggcgaa
    22261 aaaacgtctc tgcggtaaat tcgaaccgct ctccagcgaa ccgggcgcgc tggccccgct
    22321 gatggataaa ggcgagcagt tggcctgggt ctggcgcagc aaagcgcgct gtaacccgtt
    22381 gtttatcgct accggccatc gggtcagcgt ggacagcgcg ctggcgtggg tacaacgctg
    22441 catgaaaggc tatcgtctgc cggagccaac gcgctgggcg gacgcggtgg cctcggaacg
    22501 tccggcgttc gtgcgctata cagcaaatca gccctaattc aggtaaactg cggataattt
    22561 ccgtatttga gaactcaaca tgttacaaaa cccaattcat ctgcgtctgg agcgcctgga
    22621 aagctggcag catgtcactt tcatggcttg cttatgcgaa cgcatgtacc ccaattacgc
    22681 catgttctgc cagcaaaccg gttttggtga tgggcaaatt taccgtcgta ttctcgatct
    22741 catctgggaa acgctgaccg ttaaagacgc aaaagtaaat ttcgacagcc aactggagaa
    22801 atttgaagaa gcgattcctt cagccgacga tttcgatctg tacggcgttt atccggcaat
    22861 cgatgcctgc gtggcgttaa gtgaactggt ccattcgcgt ttgagtggtg aaacgctcga
    22921 acacgcggtg gaagtgagta agacctccat cacgaccgtt gcgatgctgg aaatgactca
    22981 ggctggtcgc gaaatgagcg atgaagagct taaagaaaac ccagctgtag agcaagaatg
    23041 ggacattcag tgggaaatat tccgactttt agctgagtgc gaagaacgcg atatcgagct
    23101 gataaaaggc cttagggcag acctgcgtga ggcgggtgag agcaatattg gtataatttt
    23161 tcagcaataa gaccagaaaa cgtgatttaa cgcctgattt gtcgtacctg gagtcttccc
    23221 tttcgccccc cgtctggtct acatttgggg ggcgaaaaaa agtggctatc ggtgcgtgta
    23281 tgcaggagag tgctattctg gcatttccgt cgcactcgat gcttagcaag cgataaacac
    23341 attgtaagga taacttatga acaagactca actgattgat gtaattgcag agaaagcaga
    23401 actgtccaaa acccaggcta aagctgctct ggagtccact ctggctgcaa ttactgagtc
    23461 tctgaaagaa ggcgatgctg tacaactggt tggtttcggt accttcaaag tgaaccaccg
    23521 cgctgagcgt actggccgca acccgcagac cggtaaagaa atcaaaattg ccgcagctaa
    23581 cgtaccggca tttgtttctg gcaaggcact gaaagacgca gttaagtaag attgcgtggc
    23641 agtgaacagt tttaacgaag gggtggtttc accccttttg tctttctggc gtcgatcatt
    23701 gatgctggca ggtgcgctgt ttctcactgc ctgtagccac aactcttcac ttcctccctt
    23761 taccgccagt ggatttgctg aagaccaggg cgcggtacgc atctggcgaa aagacagcgg
    23821 cgataatgtg catctgcttg ccgtgtttag cccgtggcgc agtggcgata ccacgacgcg
    23881 agagtatcgc tggcagggcg ataacctcac gctcatcaat atcaatgttt acagcaaacc
    23941 gccggtgaac attcgcgcgc gttttgacga tcgcggtgat ctgagcttta tgcaacgtga
    24001 atccgatggg gaaaagcagc agctttctaa cgaccaaatc gatttatacc gttatcgtgc
    24061 tgatcagatc cgccagatta gcgatgcctt acgtcagggg agagtcgtgc tgcgccaggg
    24121 gcgctggcat gcgatggaac agaccgtgac cacctgcgaa gggcaaacca ttaaacctga
    24181 tttagattcg caggcgatag cgcatatcga gcgccgccag agccgctctt ctgttgatgt
    24241 cagcgtggca tggctggaag cgcccgaagg ttcgcaatta ctgttagtgg caaactctga
    24301 tttctgtcgc tggcaaccca acgagaaaac gttctgattt accagtggcc catacccata
    24361 tgaccgccac caccgcagcc gccgtagccc atgcccattc cggctccgcg cggaataccc
    24421 gcttcagcca tcgcgatatc tcgtttcacc cgtaactcat ctaacgactg acgcaaattc
    24481 tccatctctt tggcgaccgc gttaattttg ctgctatccg gtgggttcgc ggctaacagg
    24541 gcattgtatt cataacgctt cgtcaccagt tgctgttgca gtgcgctgct ttgagcgtaa
    24601 aagtcattat ggattttctg ccacgctgtc tgttgttcgc tggtcaaagg cgcggcattt
    24661 tgctgccaca taccgtgtcc gccgtgagca aatgcagatg tcgatcccat cgccattgct
    24721 gaaagcgcca tcattaccag ggcaattttc gtgttccgtt tcatggttaa tcctccagtg
    24781 gttgtcttac ttcgggtatt gcatcttccg tgccaacgat gaaacgctga tatgacgggt
    24841 aatctggcat gataaacgag taaaaatgac tcgcctgctg cgggtagcga gtcattttta
    24901 ctcattgaaa cttaagcctt tgtgttacag cgcagggtaa gcgctgataa aagatggcat
    24961 gatttctgct gtcagaaagg gatgagcagg caaagaagaa gatgcgtttt atgcaacgtt
    25021 ctaaagactc cttagctaaa tggttaagcg cgatcctccc cgtggtcatt gttgggctgg
    25081 tagggttgtt tgcggtgacg gtgattcgcg attatgggcg cgagactgcc gccgccagac
    25141 aaacgctgct ggaaaaaggc agtgtactta ttcgcgctct tgaatccggc tcgcgcgtcg
    25201 gcatggggat gcgcatgcat catgcgcagc agcaggcctt actggaagaa atggccgggc
    25261 agcctggagt acgttggttt gcggtcacgg atgaacaagg aacaatcgtg atgcatagca
    25321 actccggcat ggtgggaaaa cagctttatt ccccgcagga aatgcagcag ttacatcctg
    25381 gagatgaaga agcgtggcgg cggatcgata gcgcagacgg cgagcctgtt ctggaaattt
    25441 atcgccagtt tcaaccgatg tttgctgctg gaatgcaccg gatgcgccat atgcagcagt
    25501 atgccgcgac accacaagca attttcatcg ctttcgacgc cagtaatatt gtgagtgccg
    25561 aagatcgtga gcagagaaac accctgatta tcctcttcgc cctggcgacg gtcttgctgg
    25621 caagcgtgtt gtcattcttc tggtatcgcc gctatctgcg ctcgcgccag ctgttgcagg
    25681 atgaaatgaa gcgcaaagag aagctggtag ctctggggca tcttgcggca ggcgttgccc
    25741 acgaaatccg taatccactt tcctcaatta aagggctggc gaaatacttt gccgaacgcg
    25801 cgccagcagg gggagaagcg catcaattgg cgcaggtgat ggcgaaagaa gccgaccgtt
    25861 taaaccgcgt ggtaagcgag ttgctggaac tggttaagcc aacgcatctg gctttgcagg
    25921 cggtggatct caacacgctg attaaccact cattacagct ggtaagccag gatgcaaaca
    25981 gccgggagat ccagttacgc tttaccgcca acgacacatt accggaaatt caggccgatc
    26041 cggacaggct gactcaggtc ctgttgaatc tctatctcaa tgctattcag gcgattggtc
    26101 agcatggcgt gattagcgtg acggtcagcg aaagcggcgc gggcgtgaaa atcagcgtta
    26161 ccgacagcgg taagggaatt gcggcagatc agcttgaagc catcttcact ccgtacttca
    26221 ccaccaaagc cgaaggcacc ggattggggc tggcggtcgt gcataatatt gttgaacaac
    26281 acggtggtac aattcaggtc gcaagccagg agggaaaagg ctcaacgttc accctctggc
    26341 ttccggtcaa tattacgcgt aaggacccac aaggatgacg cacgataata tcgatattct
    26401 ggtggtggat gatgacatta gccactgcac tattttgcag gctttactgc gcggctgggg
    26461 ctataacgtc gcgctggcga acagcgggcg acaggcgctg gagcaggtgc gggaacaggt
    26521 ttttgatctt gtgctttgcg atgtgcgaat ggcggagatg gacggcatcg ccacgctgaa
    26581 agagatcaaa gcgttaaacc cggcaattcc ggtgctgatt atgactgcgt actccagcgt
    26641 cgagacggcg gtagaggcac tgaaaactgg ggcgctggat tatctcatca agccgctgga
    26701 tttcgacacc ctgcaggcga cgctggaaaa ggcgctcgcg catacgcaca gtattgatgc
    26761 tgaaacacct gcggtgactg ccagccagtt cggtatggtc ggtaaaagct cggcgatgca
    26821 acacctgctc agtgaaatcg ccctcgtcgc gccatcggaa gccacggtac tgatccacgg
    26881 cgattccggc accggtaaag agctggtcgc cagggcgatt cacgccagta gcgcacgtag
    26941 cgaaaaaccg ctggtaacgc tcaactgtgc ggcactcaac gaatccttgc tggaatctga
    27001 attgttcggt cacgaaaaag gggcgtttac tggagccgat aaacgccggg aagggcgctt
    27061 tgttgaggcg gacggcggca cgctgtttct cgatgaaatt ggcgatatct cgccgatgat
    27121 gcaggtgcgt ctgctgcgtg cgattcagga gcgcgaagtt cagcgtgtcg gtagcaacca
    27181 gactatctcg gttgatgtcc ggctgattgc ggcgacccat cgcgatcttg ccgcagaggt
    27241 gaatgccggg cattttcgcc aggatctcta ctatcgcctg aatgtggtgg cgattgaagt
    27301 accatcgctg cggcaacggc gggaagatat ccctctgctg gctggccatt ttcttcagcg
    27361 ctttgccgag cgtaatcgca aggcggtaaa aggctttacg ccacaggcga tggatttgct
    27421 gatccactac gactggccgg gaaatattcg tgaactggaa aacgcggtgg aacgggcggt
    27481 ggtgctgctg accggggaat atatttccga acgcgagctg ccgctggcga ttgccggtac
    27541 gccgatcccg ctggggcaaa gtcaggatat tcagccgctg gtagaagtcg aaaaagaagt
    27601 gattctggcg gcactggaga aaacgggcgg caacaaaacc gaggccgccc gtcagttagg
    27661 gatcacgcgc aaaacgctat tggcaaaact gtcgcgttag ttctgctcgc gttcgatagc
    27721 gcgccagcca atatctttcc ggcagaagca gtcgtcccag tgaatatcgg tcattaaggc
    27781 ataagcgcgt ttctgcgctt ctgccacggt atgacccagc gcggtgacgc acagtacgcg
    27841 cccgccgctg gtcactacct gctcgttatc cgccagtttc gtgcccgcgt ggaacacttt
    27901 gccgtcagcc acttcttcca gcggcaggcc gtggatcaca tcgccggtgc ggtaatcgcc
    27961 cggatatcca cccgcagcca tcaccacgcc gagagaagcg cgttcatccc actcggatgt
    28021 tttctcgtcc agcttccctt cacaggctgc caggcagagc tcaaccagat cggacttcat
    28081 gcgcagcata atcggctggg tttccggatc gccaaagcgg cagttaaatt cgataacctt
    28141 cggattgccc tgtttgtcga tcatcaggcc cgcgtagaga aaaccggtgt aggtgttgcc
    28201 ttccgccgcc atgcctttca cggttggcca gatgatacgt tccatggtgc gctgatgaac
    28261 ttcatcggtt actaccggcg cgggggagta agcgcccatc ccgcccgtgt ttggtccggt
    28321 atctttatcg cctacgcgtt tgtgatcctg gctggtagcc atcggcaaca catgctcgcc
    28381 gtccaccatc acgataaagc tcgcttcttc gccatcgagg aactcttcga taacgatgcg
    28441 atgacccgcg tcgccaaaag cgttgcccgc cagcatatcg tgaacagccg cttccgcttc
    28501 ttccagcgtc atcgccacga taacgccttt cccggcagcc agaccgtccg ctttaatgac
    28561 aattggcgcg cctttctcac gcagatacgc cagcgcaggt tctacctcgg tgaagttctg
    28621 gtattccgcc gtagggatct tatggcgggc caggaaatct ttggtaaacg cttttgagcc
    28681 ttccagttgg gccgcacctg cggttgggcc gaagattttc agcccggcgg cgcggaaggt
    28741 atcgaccacg cctttcacca gcggcgcttc cgggccgacg atggtcagat caatcttttc
    28801 gttttgtgcg aaatccaaca gcgccgggat atcggtcacg ccaatagcaa cgttttgcag
    28861 cgcgggttcc agcgcagtgc ctgcattacc cggagcaaca aaaacagtct caaccagcgg
    28921 cgactgggcc gctttccagg ccagcgcgtg ctcgcgcccg ccgttaccaa tcactaatac
    28981 tttcatctat tgctccatta atggcggaag tggcgcatgt cggtgaagag catcgcaata
    29041 ccgtgctcgt cggcggcggc aatcacttcg tcatcacgga tggaaccgcc aggctggatt
    29101 acgcaggtca cgcccgcagc ggcggcggca tcaataccgt cgcggaacgg gaagaacgcg
    29161 tcagaagcca tcgaggaacc tttcacttcc aggccttcat cagcagcttt aataccggcg
    29221 attttcgcgg agtacacgcg gctcatctgg cccgcgccaa taccgatggt catattgttt
    29281 ttggcataga cgatagcatt ggatttcacg aacttcgcca ctttccagca gaacagcgca
    29341 tcgcgcagtt cctgttcggt cggctgacgt tgggtgacga cgcgcagttc ttccgcaccg
    29401 accatcccca ggtcacgatc ctgaaccagc agaccgccgt tcacgcgttt gaaatcaaga
    29461 cccggaacac gctcgcccca ctgaccgcag gtcagaacgc gtacgttctg cttggcggcg
    29521 gtgattttca gggcttcttc gctggcggaa ggcgcaataa tcacttcgac aaactgacga
    29581 gaaatgatgg cctgtgcggt ttccgcatcc agctcgcggt taaaggcaat gatgccgccg
    29641 aatgcggagg ttgggtcggt tttgtacgcg cgatcgtaag catcaagaat ggaattgccg
    29701 atagctacac cgcaagggtt ggcgtgcttc acaatcacac atgccggctc ggcgaactct
    29761 ttcacgcact ccagcgccgc atcggtatcg gcgatgttgt tataagagag ggctttaccc
    29821 tgaacctggg ttgcggtagc aacggaggct tctttcacat tctcttctat atagaaggca
    29881 gcctgctggt ggctgttctc gccgtaacgc atatcctgct tcttaatgaa gttcaggttc
    29941 agcgtgcgtg ggaagcgacc agcggcttct ttgctttcac cgtggtacgc cggaaccatg
    30001 ctgccgaagt agttggcaat catgctgtcg taggcggcag tgtgttcgaa ggctttgatg
    30061 gcgaggtcga aacgggttgc aagcgtcagc gatccttcgt tgtcatccat ctctttaata
    30121 atggcgtcgt agtcgctgct cttcaccacg attgcgacat ctttatggtt cttagcggcg
    30181 gagcgcacca tggtcgggcc gccgatatcg atgttctcaa ccgcatcttc cagcgagcaa
    30241 ccttcacggg ccacggtctg ggcgaacgga tacaggttaa caaccaccat atcgataggc
    30301 tggatctgat gttcttccat aatggcatcg tcctggccgc gacggcccag aatgccacca
    30361 tgtactttcg gatgcagggt cttcacgcgt ccatccatca tctccgggaa accggtgtaa
    30421 tcggaaactt cggttaccgg cagacctttt tctgctaaca gacgggcagt gccccctgta
    30481 gacagcagct ccacaccgcg tgcggaaagt gcctgggcga attcgacgat accggctttg
    30541 tcagaaacac tgagcagagc gcggcggact ggacgacgtt gttgcatggt aaatcccctg
    30601 gatttgacta ttacagagag cgttagctga atttttcgcg aaaaactcag ctaacgcccc
    30661 taacggggca tccttatttt tcgcccgcat tgtaacgaaa acgtttgcgc aacgctcgcg
    30721 aatttttctc tttcaatggt gatcacaatt ttgacagtgg ttaccgtggg aaaaatacag
    30781 aaattacatt gatgattgtg gataactctg tgcgtaaaaa ggtataaagc ggggttttgc
    30841 tggggaatgc agcagtcagt catttttctg caatttttct attgcggcct gcggagaact
    30901 ccctataatg cgcctccatc gacacggcgg atgtgaatca cttcacacaa acagccggtt
    30961 cggttgaaga gaaaaatcct gaaattcagg gttgactctg aaagaggaaa gcgtaatata
    31021 cgccacctcg cgacagtgcg ctaaagcgcg tcgc
//
LOCUS       DAMZET010000035        30899 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.035, whole genome shotgun sequence.
ACCESSION   DAMZET010000035 DAMZET010000000
VERSION     DAMZET010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.035"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..755)
                     /locus_tag="POB34_001724"
     CDS             complement(<1..755)
                     /locus_tag="POB34_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HDI5918693.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQI"
     gene            complement(792..1778)
                     /gene="lpxK"
                     /locus_tag="POB34_001725"
     CDS             complement(792..1778)
                     /gene="lpxK"
                     /locus_tag="POB34_001725"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HDI5918694.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(1775..3523)
                     /gene="msbA"
                     /locus_tag="POB34_001726"
     CDS             complement(1775..3523)
                     /gene="msbA"
                     /locus_tag="POB34_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HDI5918695.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(3560..5824)
                     /locus_tag="POB34_001727"
     CDS             complement(3560..5824)
                     /locus_tag="POB34_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HDI5918696.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILAAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPSLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ECWQWLSFSPWFLLVVWRLNAWRTLPAMCVAGGLLMCWPLWQKPRPDEWQLYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(6031..6315)
                     /gene="ihfB"
                     /locus_tag="POB34_001728"
     CDS             complement(6031..6315)
                     /gene="ihfB"
                     /locus_tag="POB34_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HDI5918697.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(6475..8148)
                     /gene="rpsA"
                     /locus_tag="POB34_001729"
     CDS             complement(6475..8148)
                     /gene="rpsA"
                     /locus_tag="POB34_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDI5918698.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(8259..8942)
                     /gene="cmk"
                     /locus_tag="POB34_001730"
     CDS             complement(8259..8942)
                     /gene="cmk"
                     /locus_tag="POB34_001730"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDI5918699.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(9115..9879)
                     /gene="ycaL"
                     /locus_tag="POB34_001731"
     CDS             complement(9115..9879)
                     /gene="ycaL"
                     /locus_tag="POB34_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HDI5918700.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(10048..11331)
                     /gene="aroA"
                     /locus_tag="POB34_001732"
     CDS             complement(10048..11331)
                     /gene="aroA"
                     /locus_tag="POB34_001732"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDI5918701.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(11402..12490)
                     /gene="serC"
                     /locus_tag="POB34_001733"
     CDS             complement(11402..12490)
                     /gene="serC"
                     /locus_tag="POB34_001733"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HDI5918702.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(12689..13381)
                     /locus_tag="POB34_001734"
     CDS             complement(12689..13381)
                     /locus_tag="POB34_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDI5918703.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            13511..15271
                     /locus_tag="POB34_001735"
     CDS             13511..15271
                     /locus_tag="POB34_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HDI5918704.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            15677..16534
                     /gene="focA"
                     /locus_tag="POB34_001736"
     CDS             15677..16534
                     /gene="focA"
                     /locus_tag="POB34_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HDI5918705.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            16589..18871
                     /gene="pflB"
                     /locus_tag="POB34_001737"
     CDS             16589..18871
                     /gene="pflB"
                     /locus_tag="POB34_001737"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDI5918706.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQTDLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            19063..19803
                     /gene="pflA"
                     /locus_tag="POB34_001738"
     CDS             19063..19803
                     /gene="pflA"
                     /locus_tag="POB34_001738"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HDI5918707.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(19885..20475)
                     /locus_tag="POB34_001739"
     CDS             complement(19885..20475)
                     /locus_tag="POB34_001739"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HDI5918708.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            20575..21483
                     /locus_tag="POB34_001740"
     CDS             20575..21483
                     /locus_tag="POB34_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI5918709.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            complement(21484..22914)
                     /locus_tag="POB34_001741"
     CDS             complement(21484..22914)
                     /locus_tag="POB34_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI5918710.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGRGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(23124..24272)
                     /locus_tag="POB34_001742"
     CDS             complement(23124..24272)
                     /locus_tag="POB34_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5918711.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            24586..25212
                     /locus_tag="POB34_001743"
     CDS             24586..25212
                     /locus_tag="POB34_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDI5918712.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDTPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            complement(25248..26111)
                     /gene="dmsC"
                     /locus_tag="POB34_001744"
     CDS             complement(25248..26111)
                     /gene="dmsC"
                     /locus_tag="POB34_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HDI5918713.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(26113..26730)
                     /gene="dmsB"
                     /locus_tag="POB34_001745"
     CDS             complement(26113..26730)
                     /gene="dmsB"
                     /locus_tag="POB34_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDI5918714.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            complement(26741..29185)
                     /gene="dmsA"
                     /locus_tag="POB34_001746"
     CDS             complement(26741..29185)
                     /gene="dmsA"
                     /locus_tag="POB34_001746"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HDI5918715.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPQWASQITGIPAEKIIQLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNTGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            complement(29424..30716)
                     /gene="serS"
                     /locus_tag="POB34_001747"
     CDS             complement(29424..30716)
                     /gene="serS"
                     /locus_tag="POB34_001747"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDI5918716.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
BASE COUNT         7953 a         8093 c         7286 g         7567 t
ORIGIN      
        1 atgatttgct gctgttcagc cctcgcttcg cgccacgccg ccagtgccgg acgtttcagc
       61 cgatagtaat ctgccagcca ctgttcgcgg aatattccca ggctacgcgc actgttatcc
      121 agcatgatga tttctgcttc tgtttgcgaa acgagatcgc gctcatcatc ccagtcaggc
      181 atgacacggt gggttaaatc ataaacgcgc tggaagttgc gccgttcaat caccatcacc
      241 tttccggcag taaataaacc ttccagatgc cgtttatgcg gcttccattc ccaccagccg
      301 cttgcacctt tacgaggatg ctcaaaatcg gctgaacgta ccggcccctt atcatgaata
      361 tgctgaatta actgtgcaat ttccgcctca tgttcctgca tccaggcgtc tttgtatttc
      421 cagcccattt tttcaggtgc cagcatgcgg tggcgaataa gacgaaagtc gctacgcggc
      481 ataaagcagg cttcatgcgc ccagtattcc attaattcgc cacgcgccag agactcatcc
      541 agccactggg caggataatt tcccagacga ctgaaaagca ccagatatgg actacgggca
      601 acaatattga tggtatcgat ttgcagcaag gacatgcggg agatcgttgc cggaatatcc
      661 tccaacgacg ctcgacggcg gggtttgttt aacaggcctt gtgcggcaag gtgaagatta
      721 cgcgcatcag caagggagag gtgcggcagc gacattcatg actccatcaa tcgaacgctg
      781 ccgcggcgta actagttgcc agaagccagc gaggttagtt gcgtaagcag tttcgctggt
      841 tcatcacctg aaagctgtgc gtctacaggc aaataccacc aattttcttc tgcaaaggcc
      901 cggcatttca ccgcatcttt ttcagtcatt accagcgttt gcccggcgct taccaacgca
      961 ctgacatccg catggttcaa agactgatga tcggccagcg gtacacattt ttccggttgt
     1021 acgccacaca tcttcagcgt ggcaaaaaag cgcggcggat gcccaatccc cgccatcgcc
     1081 actacatgtt caagctgagc aacgtcacaa cgcgtaccgg tacgtaaatt caccgcctga
     1141 cccggcagca gatgcatggg gatttcaccg ctgcgaggga caccgccgtt gacgattacc
     1201 gcatcaaccg actttaagcg ccccgctcgc tcacgcattg gccccgccgg caaccaccag
     1261 ccattgccaa agcgacgcac accatcaata acgacaattt ccacatcacg cgccagacgg
     1321 taatgctgta aaccgtcgtc ggttacgatg atctgcacat cagggtgttg cgccagaatg
     1381 gcttttaccg catcagaacg aacgggagaa accgcaacag gcgcatcagt gcgttgataa
     1441 atcaacacag gttcatcacc cgcctgtgct gttgtggtat ctgccgacaa taacagcgga
     1501 taagattcag ccttaccacc atatccccgc gatacgaccc ccacgcgaat accgcgctgt
     1561 tgcaactgtt ccaccagcca gacaacgacc ggggtttttc cgttgccgcc tgcggtgaga
     1621 ttaccaacca cgacaaccgg tacgggggca cgccaggcgc gcttcagttt tagtttatag
     1681 caaagacgga tcgcgccact caccaggcca tacaaccagg agagtggcag caatagccgc
     1741 cacaaagggg attcaccaga ccagattttt tcgatcattg gccaaactgc attttgtgaa
     1801 gttgcgcgta aacgccgcgg tgctcaagca aatcgttatg cgtaccgcgt tccacaatga
     1861 caccatcctc gacgaccacg atttcgtctg ccttttcaat ggtagacaag cggtgggcaa
     1921 tcaccagaga ggtacggttt ttctgcaact catccagtgc cgcctgaatc gcacgttcgg
     1981 attcggtatc cagagccgag gtagcttcgt ccagaatcag aatcgggcta tcacgcaaca
     2041 aggctcgagc gatagcaata cgctgacgct gaccgccaga gagcagcacg ccgttttcac
     2101 caatcactgt atcgagaccg ttatccatct tattgatgaa gtccatggcg taggccatac
     2161 gcgccgcttc ttcaatttgc tcacggctgt actgttcagt ccgtgcgtaa gcaatgttgt
     2221 tagcaaccgt atcgttaaac agatggacat tctgcgacac cagagcaacc tggttacgta
     2281 acgacgccag ggtatactcg cgcagatcgt gaccatccat caggatttcg ccttcatcaa
     2341 tatcgtaaaa acgcgtgatc aggctggcga tggttgattt acccgaacca gagcgtccaa
     2401 ccagagcaac cgtcttccct gccggaattt tcaggttgat gttacgcaat gcaggtacgt
     2461 cacgtcccgg ataagtaaag gtgacattgc ggaattccac gtcgccagtc gcacgctcga
     2521 tcacgcgctt accttcatct ttctcctgct cactgtccag aatggtaaac agcgtctgac
     2581 aagccgccat accgcgctgg aactgggcgt taacgttggt cagcgatttc agcggacgca
     2641 tcagtgcaat cattgaagag aaaacaacgg taatcgtacc ggcagtcagg ctatccatga
     2701 cacttgggaa gctcgccgca tacagaacaa acgccagcgc caaagaggcg atcagctgaa
     2761 tgatcggatc agagatggaa gaggctgaaa ccattttcat cccctgaaga cgcattcggt
     2821 tgctgacttt atcaaagcgt ttcgtttcca cttcctgacc accgaaaatc aatacttctt
     2881 tgtggccctt cagcatttgt tcggcgctgg tggtcacctg ccccatggtg ttctgcatgt
     2941 ttttactgat gttgcgaaaa cgcttcgata ctacgcgaat cgcaatcgaa acaatcggtg
     3001 ccagcacaat caaaatgatc gacagttgcc aactgtaata gaacatcatg atgaacaggc
     3061 cgatgattga cgcaccttca cgcacaaccg ttatcagtgc gccggaagaa gaagaagcaa
     3121 cctgttcgga atcgtaggtg atacgtgaca acagcgtccc cgttgactgt ttgtcaaaaa
     3181 atgaaaccgg cattcccatc atgtgaccaa acaggcggcg acgcatggtc attaccacct
     3241 ttcctgatac ccaggagata cagtagctgg agacatagct ggtgatacca cgtaaaatca
     3301 tcagcccgat caccaccagc ggcatccaca ccagcacgga gcgatctgtt ttaccaaagc
     3361 catcatcaag aagtggctta aggagcgata acatgaaggt atcgctggct gcgttgagga
     3421 ttaacgctac gcccgccacg atcagacccg ctttaaaagg cgcaatggtt ggccacagtc
     3481 ggcggaacgt ctgccacgta gagagatctt tgtcgttatg cattcaaaaa accagcattt
     3541 gttgaaatag ccgcatattc tacccgttat ccactggcac gccaaaccac tgatgatacc
     3601 aacgaggtaa aatttgctct ctgaggctgc taatccgcca gccttgcgct gaaaaattga
     3661 ccgtcacttg accctgatgt ggagtatcaa gccattgata tccttgctgt tgatagcgat
     3721 gcttaacttt attagagggc aatcgccatg cgttatagcg cgatgccgat gcgagtgcca
     3781 cttttccatt cactcgctga attaatggca acgatgatga ggtattactg ccatggtgag
     3841 gtacctgaag caatgttgtc tgcacttgct gccagtaacg gcttagcatc ttttgttcag
     3901 cggggacttc aatatcaccg gttagaagaa tgctattcgt cccgtcatca accttaacca
     3961 cacaggaatg gttatttcct ttatcgttgc tagcttgtaa aggccagtgc acgctgaaac
     4021 gcaatccttg ccattgccac gcttcgccac gcacacaggg ctgatgatgt tcccagttta
     4081 acggacttct gatccataac atcggccatg tgtgcaatat tgagtccagc cctccccggt
     4141 gatccagatg ttcatgactc agaataacgc cttccggttc aagattatgc cagtggagcc
     4201 aggggataat cagttgttgc ccactatccc cttcgggcca ggccagtcct gtgtcataga
     4261 gaatcgcttt gccgtttctg gctatcacca ttgccagccc ttgcccgaca tcaagcatgt
     4321 acagctgcca ctcgtcaggt cgaggttttt gccacagcgg ccagcacatc agcaagcctc
     4381 cagccacaca cattgctggc aacgttcgcc aggcgtttaa tcgccatacc accagtaaga
     4441 accatgggga aaatgatagc cattgccaac attcagcaat gttgatccac ccttccggca
     4501 aactctttaa cccccagaaa agtaaagcca aagaccggtc ggcaagaaac cataaccctt
     4561 gctccaggat taacggcccg cttaaatgca caaccatcgc ggcgaggatc aacggaaccg
     4621 tgataaatgt caccagggga attgctaata gatttgcaat aaacgaggtc agactaatgc
     4681 catgaaatat gacgatttgc acgggcataa gcagaagtgt gattcccagt tgcagatgga
     4741 tgagggaaac aactgcacgc aataccggcg gcagttgcca ctcaggacag ggaaaccact
     4801 gataccaaaa tatcaatgcc gcgaccgcag cggcagagag ccataaactt tgcgagagaa
     4861 tggcaacagg atccatcagc aaaattgccg ccagacaaca tatccataca tcccagccac
     4921 tccactgtcg cccacttaac ttaagcattc cccacgtagc aagcgccaca acggtacgca
     4981 atgcaggagg ttgcattccc gtcagccagg cataaaaagc agcacagcag attccgccaa
     5041 ttaatggcat ttgccagtgg atccagcgcc caggcagaaa aatttgtcca ctgcgaatga
     5101 gtccggcagc cagcaacgcc gcaaaagcga tgtgcaatcc cgatatcgcc attaaatgcg
     5161 ccgttccagt atcacgcatg atatttttga tttctttagg gacagataac cgttccccca
     5221 tacctaaacc aagaataacc gcattccacg gatagggttg cagcgttgtt tgtagtgacg
     5281 ccagatactg tgcacgcagg ctacaattcg gttcaataac acttgcctgc agaaagcggc
     5341 cggtgagcgg ctgatgctgg gcaatggcat aacgctggct atcaaagccg ccatcattaa
     5401 gttgaccatg aacggcacga actttgagtt tcattgacca cagttgtccg gcacaaaccg
     5461 cttgcggaag atattcgcca tacagcacaa ggcttggcgc agggaatata cgtcgacctt
     5521 gtagatgagt aatttgaccg taatgagtgg tcataccgtc agtagcagtg atctcaacaa
     5581 ttgcatcctg cgtcgcgcca gttaaggttt ctcctgccca caaaatttgc tttgctgcta
     5641 atatgcccca aacgaaaaac aacaacgtca gcgcgatata acggacggtt ttaacaggga
     5701 taaatgccag tacgcaggcg aagagagtca gaaacgcaag ggttaatgtc ccaggcaatt
     5761 ggggcaaaat cagcaacgga aaaattccgc aaattatgca tacgccgact gtcgttattt
     5821 tcatgcaccc ctccctgttt atggaaagtg tgcaactttg ttgagcggtt gtctggtggg
     5881 aattttgcgt tccccgaggc acgttcagaa gaatttaacg agagggtgaa aactgctcaa
     5941 aactctgcga ggcagcttcc agttcctgat tagagaaatg aaaaaagcac ccgacaggtg
     6001 cttttctcgc gttcaagttt gagtaaaaac ttaaccgtaa atattggcgc gatcgcgcag
     6061 ttctttacct ggtttaaagt gaggaacgta ttttccttcc agttctactt tatcgccagt
     6121 cttcggatta cgtccggtac gtggtgcgcg gtagtgcaaa gagaaactgc cgaaaccgcg
     6181 gatttcaata cgctcgccct gcgcaagagt cgaggccata tgctccagca tctcttttac
     6241 tgcatcttca accgtcttgg cgggaatgtg cgattgctgg gtggcaagtc tttctatcaa
     6301 ttctgacttg gtcatgattc ctccggttcc ttaaaggcaa attaagcggc tgctgttgct
     6361 gcattgatta agggcggccg tagccgccct tagtgcttga ttacaggacg aaacctgcaa
     6421 tctgtcaagt aaactcaaca aacttcggaa taaaaatccc gaagagtcag agaattactc
     6481 gcctttagct gctttgaaag cttcagccat tgcgttgttg gagaagtttg catcttcctg
     6541 tttgttaaca gttgcgattg catctttctc gtcagcttcg tctttcgcac gaacagacag
     6601 gctgattgcg cggtttttac gatcaacgcc ggtgaattta gcttcaactt cgtcgccaac
     6661 gctcagaacc agggtagcgt cttcaacgcg gtcacgggat gcttcagaag cacgcaggta
     6721 accttcaacg ccgtcagcca gttctacggt tgcgccttta gcgtcaactg cagttacttt
     6781 accggttacg atagcgcctt tcttgttcag agcaacccag ttgttgaacg gatcttctgc
     6841 gagctgttta acgcccaggg agatacgttc acgttctgcg tcaacctgca gaacaactgc
     6901 agcgatttcg tcgccttttt tgtattcacg aactgcttct tcgcctgcaa cgttccagga
     6961 gatgtcagac aggtgaacca ggccgtcgat gccgccgtcc aggccgatga agataccgaa
     7021 gtcagtgata gacttgattt taccttcaac acggtcgccc ttgttgtggg tttccgcgaa
     7081 ctgctgccac gggttagctt tgcactgttt cagacccagg gagatacgac gacgttcttc
     7141 gtcgatatcc agaaccataa cttccactac atcgccaacg ttaacaactt tggacgggtg
     7201 gatgtttttg ttggtccaat ccatttcgga aacgtgtacc aggccttcaa cgccttcttc
     7261 gatttcaacg aagcagccgt agtcggtcag gttggtcacg cgaccagtca gtttggtacc
     7321 ttccggataa cgtttagcga tagctaccca cggatcttcg cccagctgtt tcaggcccag
     7381 ggatacacgg gtacgttcgc ggtcgaactt cagcacttta acagtgattt cgtcgcccac
     7441 gttgacgatt tcgctcggat gcttaacgcg tttccaggcc atgtcagtga tgtgcagcag
     7501 gccgtcaacg ccgcccagat caacgaatgc accgtagtca gtgaggttct taacgatacc
     7561 tttaacttcc atgccttcct gcaggttttc cagcagctga tcgcgctctg cgctgttttc
     7621 ggattcgata acggcacgac gagaaacaac aacgttgttg cgcttctgat ccagcttgat
     7681 tactttaaat tcaagctctt tgccttccag gtgcagagtg tcacgcaccg gacgaacgtc
     7741 taccagagaa cctggcagga acgcacgaat accgttcagc tcaacagtga agccgccctt
     7801 aactttgccg ttgataacac cggtaacagt ttcagcatct tcgtaagctt tttccagcgt
     7861 gatccaggct tcgtgacgtt tagctttctc acgggacagc agagtttcac cgaagccgtc
     7921 ttctactgcg tccagagcaa cgtcaacttc gtcacctacc tggatttcca gctcgccctg
     7981 ggcgtttttg aactgctcag ccgggatggc ggactcagat ttcagaccag cgtcaaccag
     8041 tactacgtct ttgtcgatag caacaacaac gccacgaacg atagaacccg ggcgggtttc
     8101 gatttctttt aaggactctt caaagagttg agcaaaagat tcagtcatgt ttaatcttca
     8161 ggtttatatt taacgtccac ctggctccat gccggatggg gttgtttaac atacccgctg
     8221 tcattccatt gcaacggggg tactgcaaat tcggtcgctt atgcgagagc caatttctgg
     8281 cgcgcgtatt gtagcgcttt ttcaatcact tgctcaatgc ttaaggtggt ggaatccaac
     8341 actaaagcat cggctgccgg aaccagtggc gctaccgctc ggttacgatc gcggtcgtcg
     8401 cgttctttga tctcggccaa aaggcgctca aagttaacac taaagccctt ctcctgcaac
     8461 tgtagcatgc ggcgatgcgc acgttcttcc gaggaggcgt caaggaaaat tttcactggc
     8521 gcatcaggga ataccaccgt tcccatgtcg cggccatcgg caatcaggcc tggtaattcg
     8581 cgaaacgcgc gttggcgacg caataatgct tcacgaacgc gtgggaatgc cgcgacttgt
     8641 gaagctgcat tcgccacttc ctgagtacga atttcgccgc tgacatcttc cccttcgagg
     8701 atcacttcca gattgccatt ggttgacaca aaacgtacat ccagatggga tgccagcggt
     8761 accagcgcat cttctgacgc aacatcaaca tggtgatgta atgccgccaa tgccagtacg
     8821 cgataaattg cacccgaatc cagcagatgc cattgcaatg cttccgccat agccttacac
     8881 aaggtgcctt tccctgcacc gcttgggcca tcaatggtaa taaccggggc aattgccgtc
     8941 atctttatct ccttaaaaca ggcgtaccgt taacataacc gcgcgcatta tacgcgccga
     9001 cgccacaaat cgttaacgtt gtgtgcaaaa accagttttg tctgtattca gatggggatt
     9061 tattgcgcaa ttatagcggg ctggcagaag accagcccga aagatgacaa tgatttactt
     9121 accagaggcg atacgatcac ggatgtgttg cgcacgctct gttgatggtg ggtgagagtc
     9181 aaacatggac tgggtgcgac cgccatccag actagccagt ttttcaaagc tgccaaccag
     9241 cccctgggtg ctgatgccac gtttcttcaa cagatcaaag gagaaatcat ctgcttcgga
     9301 ctctttatca cgggagtatt tagcattaat agcgccttct gcgatatcac ccaattgtga
     9361 gcgggaaagc tgggaagcca ctccgctggt agctgaaatg gcatcgcgtg cggcaacgat
     9421 cgcataagaa gctttcattt cagccagcga gtgacccaac gcgacgtggc ccagttcatg
     9481 gcccagaaca ccttcaattt cgttgtcatt catcatgtcc atcaggccac tgtagacacg
     9541 gacacagccg ttcgccatcg cccatgcgtt gacgtcgctg gtcatataaa ccttatagtt
     9601 gaccggcgtg ccattaatat tgttacccaa tgctttggcg atttttgcca gacgtttacc
     9661 gtacttgctg gatttacttg cgacttgatt gccgctgtcc atttcggcac agccctgatt
     9721 ggcaatcgct tttgcatcgg catcgcttaa tgttgctgct ttataggcat ttaaaccgga
     9781 gctgatagcc atattggtat caataccgtg ggtattttga caccctgtca gtaatgctgc
     9841 agaggtcgca atcgccagca gtaatttagt attcttcatg tgaatcatcc gttgattaat
     9901 catatataaa tattatgtat caacaactta cattgacaag cgacgctacc attaatcaaa
     9961 atgactcaca aggtccgaaa aaaaacgccg acaatgtgcc gacgttttga taaagaaaga
    10021 tttggctatt tattgcccgt tgttcattca ggctgcctgg ctaatccgcg ccaactgctc
    10081 gaaataatcc ggaaatgttt tggccgtaca tttgggatca agaatcgtca ctggtgtatc
    10141 tgacaacgcc accagcgaga aacacatcgc catccggtga tcattgtatg tcgcgatctc
    10201 ggcaaagttc agtttttccg gtggagtgat acgaatgtaa tcgtgcccct cttctacttc
    10261 cgcaccgact ttacgcagtt ctgttgccat cgcaaacagg cgatcggttt ctttaacacg
    10321 ccagttatag atattgcgca gcgtggtggt gccttttgca aataacgccg ccgtggcaat
    10381 ggtcatcgcc gcatcgggaa tatggttcat atccatatca atagcgttca gttcaccacg
    10441 cgtgcaggaa atataatcat cgccccagca aatggtcgcg cccatttttt ccagcacatc
    10501 agcaaagcga atatcaccct gcatactgtt acgtccaata ccggtcactt ttacagtgcc
    10561 gcctttgatt gctgctgctg ccagaaagta agaagccgaa gatgcatcgc cttcgaccaa
    10621 ataagtaccc ggagactgat aagactgccc gccttttacg acaaattgtt gatagtgctg
    10681 attttcaatt tcaacaccaa acgtcttcat cagattgagt gtgatgtcga tataaggttt
    10741 agaaaccaga tcgcctttaa tacgaatcac cgtatcttcc ggcgcaagag gcgcagtcat
    10801 taacagtgcg gtgaggaatt ggctggaaac ggagccatca acgtcaacgt tgccgccagt
    10861 aaagccgccc tgtaaacgca acggcggata attttcttgt tccaggtaag tgatcttcgc
    10921 cccgcccagg cgcagcgcat ccaccagatg accaatcggg cgttctttca tacgcggctc
    10981 accggtcagc acaatatcat tgctacccag acaaagagct gccgccagcg gacgcattgc
    11041 cgttccggcg ttaccgagga acaactccag ggcaccttct gcgtgtaatg gaccgccgtt
    11101 accgataatt tcgcaacgcg tacgatcggc tgaaagcgta tagcttaccc ctaacgctgt
    11161 taatgcattc agcatatggc gcacgtcatc gctatccagc agattggtta atactgtttt
    11221 gccgtgtgct aatgccgcca gcaataaagc gcggttagaa acgctcttgg aaccgggcag
    11281 attaatagtg ccatcgacac gagcgatggg ttgtaacgtc agggattcca tgaactcaac
    11341 tctctacaac agaaataaaa accccacagg ctggctgtgg ggattaagca aaatttcggc
    11401 attaaccgtg acggcgttcg aactcaacca tgaagtctgt cagcgcttta acgccttcca
    11461 gcggcatggc gttataaata gaagcgcgca ttccgccgac cacacggtga cctttcagtg
    11521 catgaaggcc agcagcaaaa gactcttcaa ggaacaattt gtcaagcgca ctgtccgcca
    11581 actggaacgg cacgttcatc cgcgaacggt tagctttcgc cacgtcattg cggtagaaat
    11641 cgctgttatc aatcacccca tatagcagtt ctgctttttg ctgattgatt ttatccattt
    11701 cagctacacc gccgttcgct ttcagccatt taaagaccag accagataga taccaggcaa
    11761 atgtcggcgg cgtgttaaac atggagtcgt tatcgttgag gatggaataa tccagaatcg
    11821 acggacacgc gatattcgct ttgcccagca aatcttcacg aacgatgacg attgtcaggc
    11881 cagccgggcc gatatttttc tgcgcgccag cgtaaatcac gccataacgg ctgacgtcaa
    11941 tcggacggga aagaatggtt gaagagaagt cggcggcgac caccacatct ttgccgaagt
    12001 ctggcgtttc gtcgatggcg ataccgtcga tggtttcatt cgggcagtaa tgcatataag
    12061 cagcattatc agagagttgc cattcacgca ttggcttaac cgcgcgcaga ccatcaacag
    12121 tcactttggc gtcaaagaca ttaggcgtgc agtatttttt cgcttcttta atggcacttg
    12181 ccgcccagta accggcatca acataatctg cggtggtttt atcaccgaga atattcagcg
    12241 gtaccgcagc aaactgaccg cgaccaccgc catggcagaa taataccttg tagttggagg
    12301 ggacattaag aagatcgcga aaatccttct cggcttcctc tgcaacctga atgaactctt
    12361 tgccacggtg actcacttcc atcaccgacg taccaagacc gttccagtcg cgcagttcct
    12421 gttgagcctg tttaagcacc tctgccggta gcattgccgg accagaacta aaattgaaga
    12481 tttgagccat ttcccctcac cacgttgcgt tgctattaac ccgcggtcaa tcgattgcga
    12541 ccacaggtaa tacattattc ctgtggctat cggttttatc attcagtgac acacatcgca
    12601 atgagtaaaa ccgttgaggg caaaaatgtg gcttgcatca cacaaaagaa tctctcggat
    12661 tattagcaaa aagccgatgt ttttactgtc aggtcacccg ttttgcccga cttgccttcg
    12721 tccattctgg atttgcacag cgcggacata attgtttttc ccccgctttc atatgaatga
    12781 tttcactaca acgaatacag gcatagtccg ccgactccgg caccactttg taacgctgcg
    12841 tacaatcact gggtaaaata agtaaacccg gtttcacctt gtcatcttca aagaaataga
    12901 cactgatttg cccgttggtt tcgagaatcg ccagacgtac ctgccccagc tgctccacgc
    12961 cacgcaatcg tagctccata aagaactcaa attccgtcat gttggagtta ttgagtttcg
    13021 accaggccag ctcgccatct tcaataataa caaccggctt accttccagt aaatcttcca
    13081 gtttttcact gtgcgccatc aaccacatta ccaggcggta taacaacgcc agagtgataa
    13141 agacgataag taccggcacc atcggcacat catcataaaa cgccacatct cccgccgctg
    13201 aacccagcgt gagaatgatt aacacttcaa acagcgacat ctgtcgcaca ccgcgtcttc
    13261 cggtcatttt gaggaacaaa aaaaccagta caaaggtata aagactacgt agtgcaactt
    13321 cgccaaggaa atcaaaaggc actttatcaa atgccatacg gtggagatcg aacgccttca
    13381 tttttatgct cttatcgtgg tttatccgct aaaagcatag tcaacagttc atcggcatgg
    13441 cggcggtgat agcgcctttg agataaaacc cgtattattg cgcgctttcc gtacgactaa
    13501 agtgattttc atgacgcaaa catttatccc cggcaaagat gccgctctgg aagattccat
    13561 cgctcgcttc cagcaaaaac tttcagacct cggctttcag attgaagagg cctcctggct
    13621 gaatcctgtg cctaacgtct ggtctgtaca tattcgcgac aaagagtgcg cactgtgttt
    13681 taccaacggt aaaggcgcaa ccaaaaaagc ggcgctggct tctgcacttg gtgaatattt
    13741 cgagcgtctc tcaaccaact acttttttgc tgatttctgg ctgggcgaaa ccatcgccaa
    13801 cggtccgttc gtgcattatc ccaacgaaaa atggttccca ctgaccgaaa atgacgatgt
    13861 gccagaagga ctactcgatg accgtctgcg cgcgttttat gatccggaga atgaactgac
    13921 cggcagcatg ctgattgacc tacaatccgg taacgaagat cgtggtattt gcggcctgcc
    13981 gtttacgcgt cagtccgaca atcagaccgt ttatattccg atgaatatca ttggtaacct
    14041 gtacgtctcc aacggtatgt ccgcaggtaa tacccgcaac gaagcacgcg ttcagggatt
    14101 gtctgaagtt ttcgaacgct acgtgaaaaa ccgcattatt gctgaaagca tcagcctgcc
    14161 agagatcccg gcagacgtgc tggcgcgtta cccagcagta gttgaagcca tcgaaacgct
    14221 ggaagcggag ggtttcccaa tcttcgctta tgacggttcg cttggcggcc agtatccggt
    14281 gatttgcgtg gtactgttca atcctgctaa cggtacctgc tttgcctctt tcggtgcgca
    14341 tcctgatttt ggcgtagcac tggaacgtac cgtgaccgag ctgctgcaag gtcgtggcct
    14401 gaaagatttg gatgtgttta ctccgccaac cttcgatgat gaagaagtcg ctgaacatac
    14461 caacctcgaa acgcacttta tcgattccag cggtttaatc tcctgggacc tgttcaagca
    14521 ggatgccgat tatccgtttg tggactggaa tttctccggc actacggaag aagagttcgc
    14581 cacgctgatg gctatcttca aaaaggaaga taaagaagtt tatattgccg attacgagca
    14641 tctgggtgtc tacgcttgcc gcattatcgt gcctggcatg tccgatattt atccggctga
    14701 agatctgtgg ctcgcgaata acagtatggg cagccattta cgtgaaacga ttctttcgct
    14761 accaggcagc gagtgggaaa aagaagatta cctgaacctc atcgagcaac tggatgaaga
    14821 aggttttgat gactttaccc gcgtgcgtga gctgttgggt ctggcgaccg ggtcggataa
    14881 cggttggtac accctgcgta tcggtgaatt aaaagccatg ctggcgctgg ctggtggcga
    14941 tctggaacag gctctggtct ggaccgaatg gacgatggag tttaactcat cagtatttag
    15001 tccggaacgc gccaactatt atcgctgcct gcaaacgttg ttattactgg cacaggaaga
    15061 agatcgccag ccgctgcaat atctgaatgc gtttgttcgc atgtacggcg cagatgccgt
    15121 agaagccgcc agtgcggcaa tgagcggcga agcggcgttt tacggcctgc aaccggtaga
    15181 tagcgatctg cacgcgtttg ctgcacatca gtcgttgttg aaggcctacg aaaagctgca
    15241 gcgcgccaaa gcagcattct gggcaaaata aaatcaaata gcctacgcaa tgtaggctta
    15301 atgattagtc tgagttatat tacggggcgt ttttttaatg ccccgcttta catatatttg
    15361 cattaataaa ataattgtaa ttataaggtt aaatatcggt aatttgtatt taataaatac
    15421 gatcgatatt gttactttat tcgcctgatg ctccctttta attaactgtt ttagcggagg
    15481 atgcggaaaa aattcaactc atttgttaat ttttaaaatt tatttttatt tggataatca
    15541 aatatttact ccgtatttgc ataaaaacca tgcgagttac gggcctataa gccaggcgag
    15601 atatgatcta tatcaatttc tcatctataa tgctttgtta gtatctcgtc gccgacttaa
    15661 taaagagaga gttagtgtga aagctgacaa cccttttgat cttttacttc ctgctgcaat
    15721 ggccaaagtg gccgaagagg cgggtgtcta taaagcaacg aaacatccgc ttaagacttt
    15781 ctatctggcg attaccgccg gtgttttcat ctcaatcgca ttcgtcttct atatcacagc
    15841 aaccactggc acaggcacaa tgcccttcgg catggcaaaa ctggttggcg gcatttgctt
    15901 ctctctgggg ctgattcttt gtgttgtctg cggagccgat ctctttactt ccacagtgtt
    15961 gattgttgtt gctaaggcga gtgggcgcat cacctggggt cagttggcga aaaactggct
    16021 aaatgtctat tttggcaacc tggtcggcgc actgctgttt gtacttttaa tgtggctttc
    16081 cggcgagtat atgaccgcaa atggtcaatg gggactaaac gtcctacaaa ccgccgacca
    16141 caaagtgcac catactttta ttgaggccgt ctgccttggt atcctggcaa acctgatggt
    16201 atgtctggca gtatggatga gttattctgg ccgtagcctg atggacaaag cgttcattat
    16261 ggtgctgccg gtcgcgatgt ttgttgccag cggttttgag cacagtatcg caaacatgtt
    16321 tatgatcccg atgggtattg taatccgcga cttcgcatct ccggaattct ggaccgctgt
    16381 cggttctgca ccggaaaatt tttctcacct gaccgtgatg aacttcatca ctgataacct
    16441 gattccggtt acgatcggta atattatcgg cggtggtttg ttggttgggt tgacatactg
    16501 ggtcatttac ctgcgtgaaa acgatcacca ttaatggttg tcgaagtacg cagtaaataa
    16561 aaaatccact taagaaggta ggtgttacat gtccgagctt aatgaaaagt tagccacagc
    16621 ctgggaaggt tttaccaaag gtgactggca gaatgaagta aacgtccgtg acttcattca
    16681 gaaaaactac actccgtacg agggtgacga gtccttcctg gctggcgcta ctgaagcgac
    16741 caccaccctg tgggacaaag taatggaagg cgttaaactg gaaaaccgca ctcacgcgcc
    16801 agttgacttt gacaccgctg ttgcttctac catcacctct cacgacgctg gttacatcaa
    16861 caaagcgttg gaaaaagttg ttggtctgca gactgaagct ccgctgaaac gtgctcttat
    16921 cccgttcggt ggtatcaaaa tgatcgaagg ttcctgcaaa gcgtacaacc gcgaactgga
    16981 cccgatgatc aaaaaaatct tcactgaata ccgtaaaact cacaaccagg gcgtgttcga
    17041 cgtttacact ccggacatcc tgcgttgccg taaatccggt gttctgaccg gtctgccaga
    17101 tgcttatggc cgtggccgta tcatcggtga ctaccgtcgc gttgcgctgt acggtatcga
    17161 ctacctgatg aaagacaaat acgctcagtt cacctctctg cagactgatc tggaaaacgg
    17221 cgtaaacctg gaacagacta tccgtctgcg cgaagaaatc gctgaacagc accgcgctct
    17281 gggtcagatg aaagaaatgg ctgcgaaata cggctacgac atctctggtc cggctaccaa
    17341 cgctcaggaa gctatccagt ggacttactt cggctacctg gctgctgtta agtctcagaa
    17401 cggtgctgca atgtccttcg gtcgtacctc caccttcctg gatgtgtaca tcgaacgtga
    17461 cctgaaagct ggcaagatca ccgaacaaga agcgcaggaa atggttgacc acctggtcat
    17521 gaaactgcgt atggttcgct tcctgcgtac tccggaatac gatgaactgt tctctggcga
    17581 cccgatctgg gcaaccgaat ctatcggtgg tatgggcctc gacggtcgta ccctggttac
    17641 caaaaacagc ttccgtttcc tgaacaccct gtacaccatg ggtccgtctc cggaaccgaa
    17701 catgaccatt ctgtggtctg aaaaactgcc gctgaacttc aagaaattcg ccgctaaagt
    17761 gtccatcgac acctcttctc tgcagtatga gaacgatgac ctgatgcgtc cggacttcaa
    17821 caacgatgac tatgctatcg cttgctgcgt aagcccgatg atcgttggta aacaaatgca
    17881 gttcttcggt gcgcgtgcaa acctggcgaa aaccatgctg tacgcaatca acggcggcgt
    17941 tgacgaaaaa ctgaaaatgc aggttggtcc gaagtctgaa ccgatcaaag gcgatctcct
    18001 gaactacgat gaagtgatgg agcgcatgga tcacttcatg gactggctgg ctaaacagta
    18061 catcactgca ctgaacatca tccactacat gcacgacaag tacagctacg aagcctctct
    18121 gatggcgctg cacgaccgtg acgttatccg caccatggcg tgtggtatcg ctggtctgtc
    18181 cgttgctgct gactccctgt ctgcaatcaa atatgcgaaa gttaaaccga ttcgtgacga
    18241 agacggtctg gctatcgact tcgaaatcga aggcgaatac ccgcagtttg gtaacaatga
    18301 tccgcgtgta gatgacctgg ctgttgacct ggtagaacgt ttcatgaaga aaattcagaa
    18361 actgcacacc taccgtgacg ctatcccgac tcagtctgtt ctgaccatca cttctaacgt
    18421 tgtgtatggt aagaaaactg gtaacacccc agacggtcgt cgtgctggcg cgccgttcgg
    18481 accgggtgct aacccgatgc acggtcgtga ccagaaaggt gctgtagcgt ctctgacttc
    18541 cgttgctaaa ctgccgtttg cttacgctaa agatggtatc tcctacacct tctctatcgt
    18601 tccgaacgca ctgggtaaag acgacgaagt tcgtaagacc aacctggcag gtctgatgga
    18661 tggttacttc caccacgaag catccatcga aggtggtcag cacctgaacg ttaacgtgat
    18721 gaaccgtgaa atgctgctcg acgcgatgga aaacccggaa aaatatccgc agctgaccat
    18781 ccgtgtatct ggctacgcag tacgtttcaa ctcgctgact aaagaacagc agcaggacgt
    18841 tattactcgt accttcactc aatctatgta attagatttg actgaaatcg tacagtaaaa
    18901 agcgtacaat aaaggctcca cgaaagtggg gcctttttta gcacgagagc cttttttgtc
    18961 agctatctat actttaaggt gactgccaaa acagactcga cgtagccttc gagctgcgca
    19021 ccaacacggc ctcagatggg ccacatctgg agaaacaccg caatgtcagt tattggtcgc
    19081 attcactcct ttgaatcctg tggaaccgta gacggcccag gtattcgctt tatcaccttt
    19141 ttccagggct gcctgatgcg ctgcctgtat tgtcataacc gcgacacctg ggacacgcat
    19201 ggcggtaaag aagttaccgt tgaagatttg atgaaggaag tggtgaccta tcgccacttt
    19261 atgaacgctt ccggcggcgg cgttaccgca tccggcggtg aagcaatcct gcaagctgag
    19321 tttgttcgtg actggttccg cgcctgcaaa aaagaaggca ttcatacctg tctggacacc
    19381 aacggttttg ttcgtcgtta cgatccggtg attgatgaac tgctggaagt aaccgacctg
    19441 gtaatgctcg atctcaaaca gatgaacgac gagatccacc aaaatctggt tggagtttcc
    19501 aaccaccgca cgctggagtt cgctaaatat ctggcgaaca aaaatgtgaa ggtgtggatc
    19561 cgctacgttg ttgtcccagg ctggtctgac gatgacgatt cagcgcatcg cctcggtgaa
    19621 tttacccgtg atatgggcaa cgttgagaaa atcgagcttc tcccctacca cgagctgggc
    19681 aaacacaaat gggtggcaat gggtgaagag tacaaactcg acggtgttaa accaccgaag
    19741 aaagagacca tggaacgcgt gaaaggcatt cttgagcagt acggtcataa ggtaatgttc
    19801 taatttcccc gtaaagcggc aactttattg agttgccgct tttttattcc gcgcttcatt
    19861 ctgctacagc gtttttaaca catcttatag tgcatctacc atatcccgta cctgagataa
    19921 taacgactga taatagctcg cgtgaagttc ttccccatcg aataccactg tattacacaa
    19981 gaaagtgaca tcggcatctt caataccaag ataactgcac atatttttta aataatcgct
    20041 tatatttttt tcccagccca tctggacaaa tgattctttg tctcctccaa ccagcgccac
    20101 ccaacgaact ttattgaatg gtaatttgtg cccatctcca taagccagcc cattattcca
    20161 tactctgtca atatatcctt ttagcattgc cgggaagctg taccaccaga gaggaaaaac
    20221 caccactaac gtgtcatgtt caagcagctc tgaataaagc tgatgaacct ctggagaata
    20281 acgtttatcc atattcttcc agtctggttc atcttccggc gtcatcactg ggttgaaatt
    20341 acgccgatat aaatcgagtt ccgtcacctg tattttccgc tccattgccc gctgatggat
    20401 cgcatcagca atatgtgcgg tcaatgaatc atgacgagga tgggcccata ccaaataaat
    20461 acgttcagac tgcataattt tacctccacg ttaagttctg gagcgcactt tacgcaggcg
    20521 gcataatcct gtaaacgtga tgattcagga tgagttaatg aaggattttc actaatgcgg
    20581 atgaatatgt ctgactttgc cactttcttt gccgtggccc gtaatcaaag ctttcgtgca
    20641 gcgggcgatg agttaggctt atcctcgtcc gccattagcc atagtattaa aacactggaa
    20701 caacgtctta aaattcgctt attcaatcgg acaacccgaa gcgtatcgtt aacggaagcg
    20761 ggttctaatc tctatgaacg attgcgccca gctttcgacg aaattcagat catgttagat
    20821 gagatgaatg attttcgtct gacaccgaca ggaacgctca aaatcaatgc cgcccgggta
    20881 gctgcacgta tatttttaat gcctttactg gttggtttca cacgtgaata tcctgatatt
    20941 aaagtcgagc tcacaactga cgactctctt gtcgatatcg tccaacaggg ctttgacgct
    21001 ggcgttcgac ttagtggcat cgtcgaaaaa gatatgatca gcgtggcgat cggcccgcca
    21061 gtgaaattat gtgtagcagc aacgccagag tactttgcaa gatatggcaa accccgccat
    21121 cctcatgatc tgcttaatca tcaatgtgtt gtttttcgtt atcccagcgg caaacctttc
    21181 cactggcaat ttgcaaagga gctggaaatt gctgtcgcag gaaatatcat tctggatgat
    21241 gttgatgctg aactggaggc cgttttgatg ggtgctggta tcgggtatct gctctatgaa
    21301 cagatcaagg agtaccttga tactggtcgt ttagaatgtg tactggaaga ctggagcaca
    21361 gagcgtccag gcttccagat ctattatcca aaccgccagt atatgtcctg cggcttgcgc
    21421 gcatttctcg attatgtaaa aacgggtcag atctgccaga gtcagcgtca ccgaccacaa
    21481 taattattta ctattcgctt tacgcgccag cagcgggaaa atcagtccta atcctaccag
    21541 cacaaacggc gttgcaacat tcagcgccaa ctggaaggtc cactctgcag tgaaggcttc
    21601 cattttcggg aagatccccg tcaaacaggc aaaggcggta aaggcaaaac accaaatccc
    21661 gacggtcatt gccagaggct tattacgaat aaagacatat tctggtttat atttctgcgc
    21721 caggcgaacg actgcaataa atgcaacaaa tacccataaa taacgcagcg gcatcactac
    21781 cgaattaagg ttcaacagcc atttatagag attgttcata tcaccaatgc cgagagtcgg
    21841 cagcataatc agaatcgcca ccagtaccag ggtcagaaaa tagccattaa cgggcgtacc
    21901 agaagcgttg gtacgacata aactggctgg aatatatttg ctgtcagcat cacctaatag
    21961 cactttaagc ggggcatcaa tcgagaatac cagcgccgct acttgtccca gggtattcgc
    22021 aatggcgtaa atcaccatta aagtattacc catgttgtaa tactcgccca gcttctgaaa
    22081 ggcgtaatac tgaccgttgg tcattaagtc atccgggata ttacgcgaat caaacatcat
    22141 ccccatcgcc agcgagccca gaatggcaca aaccgcaacc atcaccgcca ggcataacat
    22201 cccttttgga aattcttttc ctgggttgcg cgtttgatta acgtaaggag aaatcttctc
    22261 tgctccgcca accgcgaaaa ccagcattga aatagtggta atgtaggtaa agtcgatatg
    22321 aggaatgaac gtttcccagg taatgtttgt ggtcgcaata tgcacttcag taattgcagg
    22381 cgcggttacc gccatcgcca catacaggag cgacataaca aacatcgcaa tccctgccac
    22441 agaaccgacg attttcagcg atttcatacc gcgtgaagca acccacataa agaagataaa
    22501 cagcaccagc gttaacccct gtaacgctac gactgaatat tctttgatta acgaaccgtc
    22561 gcctttcatc gcccaaccga gcgcaatcag tattgcctgg ggtttttgtg ccagataggg
    22621 aatatgcact acccagtagg tccacgcggc gaggtaagcc agtccgggtc ccatcgtgtg
    22681 tttaatccag gtactgaccc cgcccctccc atctttgaag gtcgagccta actgaccaac
    22741 aattagcgca taaggtgtga aatagagcgc aaagataaac acccatgaaa aaacaaccac
    22801 cagcccctgg ttggcgtagt tattaacaac gttgccaaaa ccccagacag tgataaaaga
    22861 catcagcgca atgttgtacc atcgcagctg tttttcctga acattcccag ccatagacaa
    22921 atccttcgcc aaaattaaag gggcatctgt aaaacgaggg attatgcgcg gcaaataaga
    22981 agtgcggaaa tagcgaaatg caaaattata acctgctgca cattttattt ttaaacgaat
    23041 aaaatttgga ataaagcaag tcagatagaa gaaacaaaaa agcagagtgt gaaacagtaa
    23101 gttcacactc tgcttgaatt catttacacg tgagcaacgg gtttcggcgt atgaccggcg
    23161 ttgcgcagca gcatcagcaa atagataaac gatacgctgg cgatcatgat aaacaataaa
    23221 ttatcggaga aattctgcat tagcatagcg gtaaatgacg ggccaagcag acttcccaca
    23281 gtatagctca acagtaaggc ctggttcatc gccaccagtt gatgatgttc aactttctcg
    23341 caagcccatg ccatcgccac cggatatagc gtaaagccag cggcaccgag gatgaataac
    23401 gctggggcca tcgccgcctg gctaagcatc gcgatactgc cgagaatgac gacaaagacc
    23461 tgaacacgca acaccagcag tcgaccaaac ttatccgcca gacgtccaat cggccattgt
    23521 ccaaggatac ccgcactgac cagtaccgcc atccagaaac caatgctggc attgctcacc
    23581 cctttgtgat tgaggtacag cggcatcagg ccatatagag atcccagaac gattcctgag
    23641 ataatgcagc cattcacgcc aagccgcgcc tgacggagtt ttagcattga cgtaattgac
    23701 gtcgaatcat ggttttcagc ctgctgattc agcacacgcg taaacaacag cggtaagatc
    23761 cctgccaacg tcaaacctgt aacccacggc aatacggaca tcagctcggt tgaaactttg
    23821 ctgaccagta actggcctaa aaacgttccc acgtaataaa ccatcatata cgcagcaagc
    23881 aaacgcccac ggttacgtga cgtcccactg cacatcagcg cgctctcaac aaccacccaa
    23941 atcatggcac agccgacgcc cgcgacaaaa cgccaagcca accagctcca gaatccaatc
    24001 atcaggccaa ggccggcaca gccagcggca aaaatgaagg aggccagata atagctgcgg
    24061 ttaaagccaa tgcgcttaat gacataccct gtcagcaatg taccgacaag gttgccggta
    24121 aaataggatg agctgacaac gcctacctgc catgtggaca tgtgttcctg ggcgagccaa
    24181 agcggcacga gtgtatttaa caccgcaatc gccagagtca acaaaagcag gccagacagc
    24241 aaaagcatga caggccgggt atacgtggac atgaattaaa accgtgagga agttcaaatt
    24301 tcatgcgcat catgccaccg ccaaaaacat tgtcaatggg tgaaaatacg cgatatccca
    24361 gcggcggtat tatcgattta tattactcat tcataacgct aatgctgaag agatgattag
    24421 tctcatctga ttgtttttat tgaactaaca caaatttcat acgcttcgtt cagtcgaaaa
    24481 atttcatggt cacagcaaac tcatatttcc cgtctatgct ttcagagcaa tgcatcgcgt
    24541 ctgatcgatt tcagacgtac cactgactct cgaaaggaga agaggatgac caaaccgtat
    24601 gttcgtcttg ataaaaatga tgctgccgtt ttgcttgttg atcaccaggc tggtttactt
    24661 tcccttgtac gggatatcga acctgataag tttaaaaata acgtgctggc gctgggtgac
    24721 ctggctaagt atttcaactt accaactatt cttaccacca gttttgaaac cggtcctaac
    24781 ggcccattag ttccagaact gaaagcacaa ttccccgata caccttacat tgctcgcccg
    24841 ggaaatatta acgcctggga taacgaagat tttgtaaaag ctgtcaaagc gacaggtaaa
    24901 aaacagttaa ttattgccgg tgtggtaacc gaagtttgcg tggcattccc ggcgctttca
    24961 gccattgaag aagggtttga cgtctttgtc gtgactgacg cgtcagggac ctttaatgaa
    25021 attacccggc attcggcatg ggatcgcatg tcgcaagctg gtgcgcaatt gatgacatgg
    25081 ttcggcgtgg cgtgcgagct gcatcgcgac tggcgtaatg atattgaagg actggcgacg
    25141 ttgttctcta accacattcc tgattatcgt aatttgatga ccagttatga cacgttaacg
    25201 aagcagaaat aaaagagaac cggggccgaa accccggttg ttactgatta gcttgcgacg
    25261 gccatcccca cggtcatatg caaaccatag aatacgccgc gaccgattaa ttcccctgcc
    25321 agaagcagaa tgaatgagac ggatagtagc ggaaccgcag gctgataacc ttttagctgt
    25381 ggtgcaatcc acaagcacag ggcaacggcc aaaagcacga tccgccagga catcagcgca
    25441 ccatagtccg gcaccagcgc tgcggcctgc tgcacagaac tatgaatagt cgccagctct
    25501 gcgccctgca tcactgacac cacgccactc actaccagtg ccagtacaga aattgctggc
    25561 agcagacgca tcgcccagcc atcgacgcca gccagactca acaacaggta acccagtaac
    25621 gggccgccca taaacatcgt caggaagaag cccatcggcg tccagatgct gtaccaggtc
    25681 ggaacggtat caatgctgtt atacacacgc accatcatcc agacaaagat gacgccaaga
    25741 accatcgtca ctatcagcca cagcgtacgc aatgccggtg acagcttttt cagcattgcc
    25801 agcaaccagc cgatgccgcc tacggcaaaa aagatcgaac cgctggcgat ttcgttactg
    25861 agtgctgaag cccctacccg gttgagcgag ttaaaagcgc gcattggtga accaagatgg
    25921 agcatagagg cgataaagcc aatgcccatc agcacccata aaccaaacat gcaggcgata
    25981 acacgctgct gggcttctgc tcgcaggtcg cctttgagca gcgccaaagc cagaacgata
    26041 aaaccacctg ctacacattg cccgaagacc gtgaagatca tcagcggcca ttcatgccat
    26101 ccacttccca tctcacacct ccttcgggtt tgccagatag ccagtggtat ccccggtcgg
    26161 gcggctattg gcattgggtt tgatcacaat attcggtttg gtaaagtgag ctcgcggcaa
    26221 cggcgcaacg gccgccagat cgccatgttt tttacgcagc tcgtcgatag ggccgaaatc
    26281 cagcgcccgc agcggacaag attcaacaca gatcggcttt ttaccctccg caacacggtc
    26341 ataacaacca tcgcatttgg tcatatggcc tttcgtttcg ttatattgcg gtgcgccata
    26401 cgggcaagcc atatggcagt agcggcagcc aatgcacaca tcttcatcga ccacaacaaa
    26461 accatcttcg cgtttatgca tcgcaccgct cgggcagact ttagtacaag ccggatcttc
    26521 gcagtggtta catgaaatcg acagatagta ggcaaacacg ttctggtgcc agacaccgtt
    26581 atcttcctgc cagtcgccgc cagcatattc ataaatgcgg cggaagctga cttctggcgt
    26641 caaatctttg tagtctttac aggccagctc gcaggttttg caaccggtgc aacggctgga
    26701 atcaataaaa aatccatact gggttgtcat cggttactcc ttacaccttt tcaacctgaa
    26761 caaggtttgt atgtgacgga ttccccttag cgagaggaga cggacgttga gtggtcagta
    26821 cgttaataca accgccctta tcgacacgtt ttgcatccgg gtcataccag gcaccttcac
    26881 ccagtgcgac cacacccggc atcattcgtg gcgtcacttt cgcctcaata tgaacttcac
    26941 cacgatcgtt aaagatcctg actttatcgc cgttgttgat accgcgtttt tgcgcatcaa
    27001 gcgggttgat ccacatttcc tgacggcaag ccgctttcag cacatcaacg ttgccgtaag
    27061 ttgagtgaac gcgagattta tagtggaaac ccgtaagctg cagcggatac tgtttgttca
    27121 gcggatcctg ataactttca aagcccggcg tgtagatcgg cagtggatcg atcacatcgc
    27181 cttctggcaa ttcccaggta gcggcaatgt cagccagcgc ctgcgaataa atctcaattt
    27241 tgcccgatgg cgtagtcagt gggtttgcct gcggatcttc acggaaggct ttataagcaa
    27301 cgtgatgccc ttgtgggtcg cgctttttaa agatcccctg cttgcggaac tcttcaaacg
    27361 ttggcagttc aggaatcgct tcccgcgact gggcatacag atgccgcatc cactcttcct
    27421 gtgtacggcc ttcagtaaac tgttgctcaa cgccaagacg ttttgccagt tcgctggtca
    27481 tttcatagat ggttttacat tcaaagcgcg gtttaatcac ctgatcgttg aaaatcacgt
    27541 aagacatatt cccgcaggat gcatccagtg caaagtccat ctgttcggaa gcggtgcagt
    27601 caggcagcag gatgtcagca tatttcgccg atgaggtcat gtggcagtcg ataaccacaa
    27661 tcagctcgca cttcttatca tcctgaagga tttcatgggt acggttgatt tcagaatgct
    27721 ggttaatcag gcaattaccg gcatagttcc agatcatttt gatcggcaca tccagcttat
    27781 ctttcccgcg cacaccatca cgtagcgccg tcatttccgg gccacgttca atggcatcgg
    27841 tccacataaa catagaaatg ctggtctgga tcgggttttc gagggtcggc atacggacaa
    27901 acggcaggct ataggaacct tcacgcgcgc cggtgttacc gccgttaatg ccgacgttac
    27961 cggtcagaat cgccagcatc gagatagcgc gagtcgcgat ttcgccgttg gcgtggcgct
    28021 gtggccccca tccctggctg ataaacgccg gtttggtact gccgatttcc cgtgccagtt
    28081 gaatgatttt ctctgccggg ataccggtga tttgcgaggc ccattgcggt gttttagcca
    28141 cgccatctgg cccttcaccc agaatataag ctttatagtg gccatttttc ggcgcgctag
    28201 cgggcagggt tttctcatcg tagccaacgc aatatttatc aaggaatgcc tgatccacca
    28261 ggttttcggt gatcatgaca tacgccagac cgttaaccag tgcggcatct gttcctggac
    28321 gaatagggat ccactcatct tcgcgcccgg caccggtgtc ggtatagcgc ggatcgatga
    28381 tgatcatgcg ggcattagat ttctggcgtg cctgttcaag atagtaagtc accccaccgc
    28441 cactcattcg cgtttcgcct gggttattac caaacagcac taccaattta ctgttttcga
    28501 tatccgacgg gctgttacca tctgcccagc cgccgtaggt atagttcaaa ccttccgcaa
    28561 tttgcgcaga agagtagtca ccgtaatggt taagataacc gccgcagcag ttcatcaggc
    28621 gcgcgaccag ggtatttccc ggcggccagg agcgggtcat ggtgccgccc agcgtaccgg
    28681 tgccatagtt cagatagata gactcgttgc cgtactcttt gatcaggcgc tgcatattgg
    28741 tcgcgatgat gtcgtaggct tcttcccagc taatgcgctc gaatttgcct tcaccgcgcg
    28801 ccccgactcg tttcatcgga tatttcaggc ggtccggatt gtagacacgg cgacgcatgg
    28861 aacgcccacg cagacaggcg cgaacctggt gcaggccgtc gtaattgtca tcgccggtat
    28921 tgtccgtttc gacatatttg atttcaccgt ccacgacgtg catacgtagc gggcagcgac
    28981 taccacagtt aactgtacag gcgctccaga taaccttttc gtctgatttt gttggaatgg
    29041 cgctatcgac agcgtgcgca atccgactaa aaggtaatgt taatgcgctg ctggccattg
    29101 ccaggccgcc gatcgctgtc gtttttacca aaccacggcg actcacctca gcagccaata
    29161 ccgcatcagg gattttcgtt ttcataatgg ctcactcaag cttgctcacg attgattcca
    29221 gtaacaaacc gtattgtgta attatttcta tagcagttcg ctatataggc ttgtatacat
    29281 cgaataattt gaaaatcaaa gagataggta gattttcact cgaaagtagt attacgtgta
    29341 agtgaggagt aattattgtt cggtatcaaa gggagacaaa aaaagcgccc gcaggcgctt
    29401 tttagattca gaaaaattgg gtattagcca atatattcca gtccgttcat atacggacgc
    29461 agaacttctg gtacttcaat acgaccatca gcctgctgat agttttccat aaccgcaacc
    29521 agcgtacgac caacagccag accagaaccg ttcagggtat gaaccagacg ggttttcttg
    29581 tccgacttgc tgcgacaacg tgcctgcata cgacgtgcct ggaaatccca aacgttggag
    29641 caggaagaga tctcacggta ggtgttctgt gccgggatcc atacttccag gtcgtaagtt
    29701 ttgcaagcgc caaagcccat gtcgccagtg caaaggatga ttttacggta cggcaggccc
    29761 agcaactgca ggactttttc tgcatgacca gtcatctctt ccagcgccgc cattgagtct
    29821 tccgggcgca cgatctgcac catttcaact ttgtcgaact ggtgcatacg gatcagacca
    29881 cgggtgtcac gaccatatga accggcttca gaacggaagc atggggtgtg ggcggtcatc
    29941 ttaattggca gatcatcttc atcgatgatt tcaccgcgca ccaggttagt cagcggaact
    30001 tctgccgttg ggatcagcgc atagttactg gtgtctgctt cttcttccag cggacgagta
    30061 tggaacagat cgccagcaaa tttcggcagc tgacccgtac cgtacagcgt gtcctggtta
    30121 accaggtacg gaacatagtt ctcactgtag ccatgctgtt cggtatgcag atccagcata
    30181 aactgcgaca gtgcgcggtg catgcgagca atctgccctt tcattaccac aaagcgggaa
    30241 ccggtcagct taactgcggc tgcaaagtcg aggccagagt gcatttcacc cagcgtcaca
    30301 tggtcacgaa cttcaaagtc aaactcacgc ggggtgcccc agcggctgac ttcgacgttg
    30361 tcattttcgt ctttacctac cggcacttca tctgcaggca ggttagggat ggtcagcgcg
    30421 atatcgcgaa tttcagcctg taaagcatcc agctcggctt ttgctgcatc cagctcttcg
    30481 cccagtttgt tcacttccag acgtaaaggc tcgatatctt ccccgcgcgc tttcgcctgg
    30541 ccaatggatt tcgatcggga gttacgctcc gcttgcaggt tttccgtttt gacctgcaat
    30601 actttacgac gctcttcaag agcgcccagc ttatctacat ccagcttaaa gccccggcgt
    30661 gccagttttt cagcgactgc gtctggctca ttacgcagca gattgggatc gagcatgctt
    30721 atcctgtgct tatcgaatta aatgggagat gtggccacag cctgcgacca cagggataca
    30781 gtaacaacat taccgcaacg ataacattaa cggtagcgtt ttatggggct attttgatcc
    30841 tgttcagcca gccaggcgag cttttcgcca atcttgcctt caaggcccct gtttgtcgg
//
LOCUS       DAMZET010000036        30591 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.036, whole genome shotgun sequence.
ACCESSION   DAMZET010000036 DAMZET010000000
VERSION     DAMZET010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.036"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            72..629
                     /gene="frr"
                     /locus_tag="POB34_001748"
     CDS             72..629
                     /gene="frr"
                     /locus_tag="POB34_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDI5918717.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            721..1917
                     /gene="ispC"
                     /locus_tag="POB34_001749"
     CDS             721..1917
                     /gene="ispC"
                     /locus_tag="POB34_001749"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDI5918718.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSCTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            2106..2864
                     /gene="ispU"
                     /locus_tag="POB34_001750"
                     /gene_synonym="uppS"
     CDS             2106..2864
                     /gene="ispU"
                     /locus_tag="POB34_001750"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HDI5918719.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            2877..3734
                     /gene="cdsA"
                     /locus_tag="POB34_001751"
     CDS             2877..3734
                     /gene="cdsA"
                     /locus_tag="POB34_001751"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDI5918720.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            3746..5098
                     /gene="rseP"
                     /locus_tag="POB34_001752"
     CDS             3746..5098
                     /gene="rseP"
                     /locus_tag="POB34_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HDI5918721.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            5128..7560
                     /gene="bamA"
                     /locus_tag="POB34_001753"
     CDS             5128..7560
                     /gene="bamA"
                     /locus_tag="POB34_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HDI5918722.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            7682..8167
                     /gene="skp"
                     /locus_tag="POB34_001754"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             7682..8167
                     /gene="skp"
                     /locus_tag="POB34_001754"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HDI5918723.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            8171..9196
                     /gene="lpxD"
                     /locus_tag="POB34_001755"
     CDS             8171..9196
                     /gene="lpxD"
                     /locus_tag="POB34_001755"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HDI5918724.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            9301..9756
                     /gene="fabZ"
                     /locus_tag="POB34_001756"
     CDS             9301..9756
                     /gene="fabZ"
                     /locus_tag="POB34_001756"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDI5918725.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            9760..10548
                     /gene="lpxA"
                     /locus_tag="POB34_001757"
     CDS             9760..10548
                     /gene="lpxA"
                     /locus_tag="POB34_001757"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HDI5918726.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            10548..11696
                     /gene="lpxB"
                     /locus_tag="POB34_001758"
     CDS             10548..11696
                     /gene="lpxB"
                     /locus_tag="POB34_001758"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HDI5918727.1"
                     /translation="MTKQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            11693..12289
                     /gene="rnhB"
                     /locus_tag="POB34_001759"
     CDS             11693..12289
                     /gene="rnhB"
                     /locus_tag="POB34_001759"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDI5918728.1"
                     /translation="MIEFVYPHTHLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            12324..15806
                     /gene="dnaE"
                     /locus_tag="POB34_001760"
     CDS             12324..15806
                     /gene="dnaE"
                     /locus_tag="POB34_001760"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDI5918729.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            15819..16778
                     /gene="accA"
                     /locus_tag="POB34_001761"
     CDS             15819..16778
                     /gene="accA"
                     /locus_tag="POB34_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDI5918730.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            16877..19018
                     /gene="ldcC"
                     /locus_tag="POB34_001762"
     CDS             16877..19018
                     /gene="ldcC"
                     /locus_tag="POB34_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HDI5918731.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            19075..19464
                     /locus_tag="POB34_001763"
     CDS             19075..19464
                     /locus_tag="POB34_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI5918732.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            19529..20827
                     /gene="tilS"
                     /locus_tag="POB34_001764"
     CDS             19529..20827
                     /gene="tilS"
                     /locus_tag="POB34_001764"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDI5918733.1"
                     /translation="MTLTLNRQLLTLRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWINSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(20876..21136)
                     /gene="rof"
                     /locus_tag="POB34_001765"
     CDS             complement(20876..21136)
                     /gene="rof"
                     /locus_tag="POB34_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HDI5918734.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(21123..21323)
                     /locus_tag="POB34_001766"
     CDS             complement(21123..21323)
                     /locus_tag="POB34_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HDI5918735.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            21489..22034
                     /locus_tag="POB34_001767"
     CDS             21489..22034
                     /locus_tag="POB34_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HDI5918736.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     VLFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            22031..22453
                     /gene="arfB"
                     /locus_tag="POB34_001768"
     CDS             22031..22453
                     /gene="arfB"
                     /locus_tag="POB34_001768"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDI5918737.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            22467..23177
                     /gene="nlpE"
                     /locus_tag="POB34_001769"
                     /gene_synonym="cutF"
     CDS             22467..23177
                     /gene="nlpE"
                     /locus_tag="POB34_001769"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HDI5918738.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDALSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(23332..24155)
                     /locus_tag="POB34_001770"
                     /pseudo
     CDS             complement(23332..24155)
                     /locus_tag="POB34_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(24208..25926)
                     /gene="proS"
                     /locus_tag="POB34_001771"
     CDS             complement(24208..25926)
                     /gene="proS"
                     /locus_tag="POB34_001771"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDI5918739.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVNAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(26038..26745)
                     /gene="tsaA"
                     /locus_tag="POB34_001772"
     CDS             complement(26038..26745)
                     /gene="tsaA"
                     /locus_tag="POB34_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HDI5918740.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(26742..27146)
                     /gene="rcsF"
                     /locus_tag="POB34_001773"
     CDS             complement(26742..27146)
                     /gene="rcsF"
                     /locus_tag="POB34_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HDI5918741.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(27264..28079)
                     /gene="metQ"
                     /locus_tag="POB34_001774"
     CDS             complement(27264..28079)
                     /gene="metQ"
                     /locus_tag="POB34_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HDI5918742.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(28119..28772)
                     /gene="metI"
                     /locus_tag="POB34_001775"
     CDS             complement(28119..28772)
                     /gene="metI"
                     /locus_tag="POB34_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HDI5918743.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(28765..29796)
                     /gene="metN"
                     /locus_tag="POB34_001776"
     CDS             complement(28765..29796)
                     /gene="metN"
                     /locus_tag="POB34_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HDI5918744.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            29984..30559
                     /gene="gmhB"
                     /locus_tag="POB34_001777"
     CDS             29984..30559
                     /gene="gmhB"
                     /locus_tag="POB34_001777"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HDI5918745.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         7347 a         7527 c         8414 g         7303 t
ORIGIN      
        1 ctatacttag cacacttcca ctgtgtgtga ctgtctggtc tgactgagac aagttttcaa
       61 ggattcgtaa cgtgattagc gatatcagaa aagatgctga agtacgcatg gacaaatgcg
      121 tagaagcgtt caaaacccaa atcagcaaaa tacgcacggg tcgtgcttct cccagcctgc
      181 tggatggcat tgtcgtggaa tattacggca cgccgacgcc gctgcgtcag ctggcaagcg
      241 taacggtaga agattcccgt actctgaaaa tcaacgtgtt tgatcgttca atgtctccgg
      301 ccgttgaaaa agcgattatg gcgtccgatc ttggcctgaa cccgaactct gcgggtagcg
      361 acatccgtgt tccgctgccg ccgctgacgg aagaacgtcg taaagatctg accaaaatcg
      421 ttcgtggtga agcagaacaa gcgcgtgttg cagtacgtaa cgtgcgtcgt gacgcgaacg
      481 acaaagtgaa agcactgttg aaagataaag agatcagcga agacgacgat cgccgttctc
      541 aggacgatgt acagaaactg actgatgctg caatcaagaa aattgaagcg gcgctggcag
      601 acaaagaagc agaactgatg cagttctgat ttcttgaacg acaaaaacgc cgctcagtag
      661 atccttgcgg atcggctggc ggcgttttgc tttttattct gtctcaactc tggatgtttc
      721 atgaagcaac tcaccattct gggctcgacc ggctcgattg gttgcagcac gctggacgtg
      781 gtgcgccata atcccgaaca cttccgcgta gttgcgctgg tggcaggcaa aaatgtcact
      841 cgcatggtag aacagtgcct ggaattctct ccccgctatg ccgtaatgga cgatgaagcg
      901 agtgcgaaac ttcttaaaac gatgctacag caacagggta gctgcaccga agtcttaagt
      961 gggcaacaag ccgcttgcga tatggcagcg cttgaggatg ttgatcaggt gatggcagcc
     1021 attgttggcg ctgctgggct gttacctacg cttgctgcga tccgcgcggg taaaaccatt
     1081 ttgctggcca ataaagaatc actggttacc tgcggacgtc tgtttatgga cgccgtaaag
     1141 cagagcaaag cgcaattgtt accagtcgat agcgaacata acgccatttt tcagagttta
     1201 ccgcaaccta tccagcataa tctgggatac gctgaccttg agcaaaatgg cgtggtgtcc
     1261 attttactta ccgggtctgg tggccctttc cgtgagacgc cattgcgcga tttggcaaca
     1321 atgacgcccg atcaagcctg ccgtcatccg aactggtcga tgggacgtaa aatttctgtc
     1381 gattcggcta ccatgatgaa caaaggtctg gaatacattg aagcgcgttg gctgtttaac
     1441 gccagcgcca gccagatgga ggtgctgatt cacccgcagt cagtgattca ctcaatggtg
     1501 cgctatcagg acggcagtgt tctggcgcag ctgggggaac cggatatgcg tacgccaatt
     1561 gcccacacca tggcatggcc gaatcgcgtg aactctggcg tgaagccgct cgatttttgc
     1621 aaactaagtg cgttgacatt tgccgcaccg gattatgatc gttatccatg cctgaaactg
     1681 gcgatggagg cgttcgaaca aggccaggca gcgactacag cattgaatgc cgcaaacgaa
     1741 atcaccgttg ctgcttttct tgcgcaacaa atccgcttta cggatatcgc tgcgttgaat
     1801 ttatccgtac tggaaaaaat ggatatgcgc gaaccacaat gtgtggacga tgtgttatct
     1861 gttgatgcga acgcgcgtga agtcgccaga aaagaggtga tgcgtctcgc aagctgagga
     1921 taatccggct acagagagtc gcgctatttg ttagcgtagg gcttcagtga tatagtctgc
     1981 gccatctgat cgtaagtagt tggctttata aggtcaggta tgccgtggtt ttacacggct
     2041 tttttttgta taggcttcag tattcctgag taccgtaaac cctgtcaggg aataaaaaac
     2101 gcgtgatgtt gtctgctact caaccactta gcgaaaaatt gccagcgcat ggctgccgtc
     2161 atgttgcgat cattatggac ggcaatggtc gctgggcaaa aaagcaaggg aagattcgtg
     2221 cctttgggca taaagccggg gcaaaatccg tccgccgggc tgtctctttt gcggccaaca
     2281 acggtattga ggcgttaacg ctgtatgcct ttagtagtga aaactggaac cgaccagcgc
     2341 aggaagtcag tgcgttaatg gaactgtttg tgtgggcgct cgatagcgaa gtaaaaagtc
     2401 tgcaccgaca taacgtgcgt ctgcgtatta ttggcgatac cagtcgcttt aactcgcgtt
     2461 tgcaagaacg tattcgtaaa tctgaagcgc taacagccgg gaataccggt ctgacgctga
     2521 atattgcggc gaactacggt ggacgttggg atatagtcca gggagtcagg caactggctg
     2581 aaaaggtgca gcaaggaaac ctgcaaccag atcagataga tgaagagatg ctaaaccagc
     2641 atgtctgtat gcatgaactg gcccctgtag atttagtaat taggactggg ggggagcatc
     2701 gcattagtaa ctttttgctt tggcaaattg cctatgccga actttacttt acagatgttc
     2761 tctggcccga tttcgatgaa caagactttg aaggggcgtt aaatgccttt gctaatcgag
     2821 agcgtcgttt cggcggcacc gagcccggtg atgaaacagc ctgatggggg tcgcttttgc
     2881 tgaagtatcg cctgatatct gcttttgtgt taatacccgt cgtcatcgcg gcgttgtttc
     2941 tgttgccgcc ggtggggttc gccattgtaa cgctggtggt ctgcatgctg gcagcgtggg
     3001 aatggggaca gcttagcggt tttaccactc gttcgcagcg agtatggttg gcggtgttat
     3061 gcgggttatt gttggcgctg atgctttttc tgttgccgga atatcaccga aatattcatc
     3121 aaccgctggt tgaaatctca ctttgggctt cgctgggttg gtggattgtc gcgctattgc
     3181 tggtgctgtt ttacccaggt tccgcagcaa tctggcgtaa ctctaaaaca ttgcgcctta
     3241 tttttggcgt gctaaccatt gttcccttct tctggggcat gctggcgtta cgggcctggc
     3301 actatgacga gaatcattac agtggcgcaa tatggctgct ctatgtcatg atcctggtat
     3361 ggggcgctga ctccggagca tatatgtttg gcaaattgtt tggtaaacat aagctggcac
     3421 cgaaggtttc tccgggtaaa acctggcaag gctttatcgg tggactcgct actgcagcgg
     3481 taatctcatg gggttatggc atgtgggcga atctcgacgt cgctcccgtc accttactca
     3541 tttgctctat tgtcgcagcg ttagcctcag tgctcggcga tctgaccgag agtatgttta
     3601 agcgtgaagc aggaattaag gacagcggtc atttaattcc aggacacggt ggtattttag
     3661 atcgtattga tagcctgacg gctgcggtac cggtctttgc ttgcttgttg ttactggtat
     3721 tcaggacgct ttaacggaag gtaatatgct gagttttctc tgggatttgg cttcgttcat
     3781 cgttgcactg ggtgtactta tcaccgtgca tgaatttggt catttctggg ttgcccggcg
     3841 ttgtggtgtt cgcgttgagc gtttctcaat agggtttggt aaggcgctct ggcggcgaac
     3901 tgataagctc ggcaccgaat atgttatcgc cctgatcccg ttgggcggtt atgtcaaaat
     3961 gctggatgag cgcgcagaac cggtcgttcc ggaactccgc caccatgcct tcaataataa
     4021 atctgtcggc caacgagcgg cgattattgc cgcaggtccg gttgcaaact tcatttttgc
     4081 tatctttgcc tactggctgg tttttattat tggtgtgcct ggcgtacgtc cggtggttgg
     4141 cgaaatagca gccaattcga tagctgcgga agcacaaatt gcaccaggta cggaactaaa
     4201 agccgtagat ggtatcgaaa cgcctgattg ggatgccgtg cgtttgcagt tggtcgataa
     4261 aattggcgat gaaagcacca ccattacagt agcgccattt ggcagcgacc aacggcggga
     4321 tgtaaagctc gatttacgtc actgggcgtt tgagcctgat aaagaagatc cggtatcttc
     4381 gctggggatt cgtcctcgtg ggccgcaaat tgaacctgta ctggaaaatg tgcagccaaa
     4441 ctcggcggca agcaaggcag gtttgcaagc aggcgacagg atcgttaaag tcgatggtca
     4501 gcccttaacg cagtgggtga cctttgtgat gcttgtccgg gataacccgg gtaaatcctt
     4561 agcgttagaa atcgaaaggc agggaagtcc cttgtctttg acattaatcc cggagagtaa
     4621 accgggtaat ggtaaagcga ttggttttgt cggtattgag ccgaaagtca ttcctttgcc
     4681 agatgagtat aaagttgtac gccagtatgg gccgttcaac gccatcgtcg aagccacgga
     4741 caaaacgtgg cagctgatga agctgacggt cagtatgctg ggaaaattga tcaccggtga
     4801 tgtgaaactg aacaacctca gtgggccgat ctctatcgcc aagggggctg ggatgacagc
     4861 ggaactcggg gtagtttatt acctgccgtt tcttgcgctt attagcgtga acttagggat
     4921 aattaacctg tttccgttgc ccgtacttga cggggggcat ctgctgttcc ttgcgatcga
     4981 aaagatcaag ggcggaccgg tatccgagcg ggttcaagac ttttgttatc gcattggctc
     5041 gattctgctg gtgctgttaa tggggcttgc acttttcaat gatttctctc ggttatgaga
     5101 gttagttagg aagaacgcat aataacgatg gcgatgaaaa agttgctcat agcgtcgctg
     5161 ctgtttagca gcgccaccgt atacggtgct gaagggttcg tagtgaaaga tattcatttc
     5221 gaaggccttc agcgtgtcgc cgttggtgcg gccctcctca gtatgccggt gcgcacaggc
     5281 gacacggtta atgatgaaga tatcagtaat accattcgcg ctctgtttgc taccggcaac
     5341 tttgaggatg ttcgcgtcct tcgtgatggt gatacccttc tggttcaggt aaaagaacgt
     5401 ccgaccattg ccagcattac tttctccggt aacaaatcgg tgaaagatga catgctgaag
     5461 caaaacctcg aggcttctgg tgtgcgtgtg ggcgaatccc tcgatcgcac caccattgcc
     5521 gatatcgaga aaggtctgga agacttctac tacagcgtcg gtaaatatag cgccagcgta
     5581 aaagctgtcg tgaccccgct gccgcgcaac cgtgttgacc taaaactggt gttccaggaa
     5641 ggtgtgtcag ctgaaatcca gcaaattaac attgttggta accatgcttt caccaccgac
     5701 gaactgatct ctcatttcca actgcgtgac gaagtgccgt ggtggaacgt ggttggcgat
     5761 cgtaaatacc agaaacagaa actggcgggc gaccttgaaa ccctgcgcag ctactatctg
     5821 gatcgcggtt atgcccgttt caacatcgac tctacccagg tcagtctgac gccagataaa
     5881 aaaggtattt acgtcacggt gaacatcacc gaaggcgatc agtacaagct ttctggcgtt
     5941 gaagtgagcg gcaaccttgc cgggcactcc gctgaaattg agcagctgac taagatcgag
     6001 ccgggtgagc tgtataacgg caccaaagtg accaagatgg aagatgacat caaaaagctt
     6061 ctcggtcgct atggttatgc ctatccgcgc gtacagtcga tgcctgaaat taacgatgcc
     6121 gacaaaaccg ttaaattacg cgtgaacgtt gatgcgggta accgtttcta cgtgcgtaag
     6181 atccgttttg aaggtaacga tacctcgaaa gatgccgtcc tgcgtcgcga aatgcgtcag
     6241 atggaaggtg catggctggg aagcgatctg gtcgatcagg gtaaggagcg tctgaatcgt
     6301 ctgggcttct ttgaaactgt cgataccgat acccaacgtg ttccgggtag cccggaccag
     6361 gtagatgtcg tctacaaggt aaaagagcgc aacaccggta gcttcaactt tggtattggt
     6421 tacggtactg aaagtggcgt gagcttccag gctggtgtgc agcaggataa ctggttaggt
     6481 acaggttatg ctgttggtat caacgggacc aaaaacgatt accagaccta tgctgaactg
     6541 tcggtaacca acccgtactt cactgtagat ggtgtaagcc tcggtggtcg tctcttctat
     6601 aatgacttcc aggcagatga cgccgacctg tccgactata ccaacaagag ttatggtaca
     6661 gacgtgacgt tgggcttccc gatcaacgaa tataactcgc tgcgtgcagg tctgggttat
     6721 gtacataact ccctgtccaa catgcagcct caggttgcga tgtggcgtta tctgtactct
     6781 atgggtgaac atccgagcac ctctgatcag gataatagct tcaaaacgga cgacttcacg
     6841 ttcaactatg gttggaccta taacaagctt gaccgtggtt acttcccgac agatggttca
     6901 cgtgtcaacc tgaccggtaa agtgaccatt cctggatcgg ataacgaata ctacaaagtg
     6961 acgttagaca cggcgactta tgtgccgatc gatgacgatc acaaatgggt tgttctgggg
     7021 cgtacccgct ggggttatgg tgatggttta ggcggcaaag agatgccgtt ctacgagaac
     7081 ttctatgccg gtggttccag caccgtgcgt ggcttccagt ccaatacaat tggtccgaaa
     7141 gcagtttact tcccgcatca ggccagtaat tatgatccgg actatgatta cgaatgtgcg
     7201 actcaggacg gcgcgaaaga cctgtgtaaa tcggatgatg ctgtaggcgg taacgccatg
     7261 gcggttgcca gcctcgagtt catcaccccg acgccgttta ttagcgataa gtatgctaac
     7321 tcggttcgta cttccttctt ctgggatatg ggtaccgttt gggatacaaa ctgggattcc
     7381 agccaatatt ctggatatcc ggactatagt gatccaagca atatccgtat gtctgcgggt
     7441 atcgcattac aatggatgtc cccattgggg ccgttggtgt tctcctacgc ccagccgttc
     7501 aaaaagtacg atggagacaa ggcagaacag ttccagttta acatcggtaa aacctggtaa
     7561 gtgttctcca caaaggaatg tagtggtagt gtagcgatga ctttaggcga tcaatataag
     7621 atcgccgggc cacgcaaaga actgcaccct ccggtgcaaa tgggatggta aggagtttat
     7681 tgtgaaaaag tggttattag ctgcaggtct cggtttagca ctggcaactt ctgctcaggc
     7741 ggctgacaaa attgcaatcg tcaacatggg cagcctgttc cagcaggtag cgcagaaaac
     7801 cggtgtttct aacacgctgg aaaatgagtt caaaggccgt gccagcgaac tgcagcgtat
     7861 ggaaaccgat ctgcaggcta aaatgaaaaa gctgcagtcc atgaaagcgg gcagcgatcg
     7921 cactaagctg gaaaaagacg tgatggctca gcgccagact tttgctcaga aagcgcaggc
     7981 ttttgagcag gatcgcgcac gtcgttccaa cgaagaacgc ggcaaactgg ttactcgtat
     8041 ccagactgct gtgaaatccg ttgccaacag ccaggatatc gatctggttg ttgatgcaaa
     8101 cgccgttgct tacaacagca gcgatgtaaa agacatcact gccgacgtac tgaaacaggt
     8161 taaataagta atgccttcaa ttcgactggc tgatttagcg cagcagttgg atgcagaact
     8221 acacggtgat ggcgatatcg tcatcaccgg cgttgcgtcc atgcaatctg cacaaacagg
     8281 tcacattacg ttcatggtta acccaaaata ccgtgagcat ttaggcttgt gccaggcgtc
     8341 cgcggttgtc atgacccagg acgatcttcc tttcgcgaaa agtgccgcgc tggtagtgaa
     8401 gaatccctac ctgacttacg cgcgcatggc gcaaatttta gataccacgc cgcagcccgc
     8461 gcagaacatt gcacccagtg cggtgatcga cgcgacggcg aagctgggta acaacgtatc
     8521 gattggcgct aacgcggtga ttgagtccgg cgttgaactg ggcgataacg tgattatcgg
     8581 tgccggttgc ttcgtaggta aaaacagcaa aatcggtgca ggttcgcgtc tctgggcgaa
     8641 cgtaaccatt taccatgaga tccagatcgg tcagaattgc ctgatccagt ccggaacagt
     8701 ggtaggcgca gacggctttg gttatgccaa cgatcgtggt aactgggtga agatcccaca
     8761 gattggtcgc gtaattattg gcgatcgcgt ggagatcggt gcctgcacaa ccatcgatcg
     8821 cggcgcgctg gatgacacta ttattggcaa tggcgtgatc attgataacc agtgccagat
     8881 tgcacataac gtcgtgattg gcgacaatac ggcggttgcc ggtggcgtca ttatggcggg
     8941 cagcctgaaa attggtcgtt actgcatgat cggcggagcc agcgtaatca acgggcatat
     9001 ggaaatatgc gacaaagtga cggttacggg catgggtatg gtgatgcgtc ccatcactga
     9061 accaggcgtc tattcctcag gcattccgct gcaacccaac aaagtctggc gcaaaaccgc
     9121 tgcactggtg atgaacattg atgacatgag caagcgtctg aaatcgcttg agcgcaaggt
     9181 taatcaacaa gactaacgtt ccatcttttg ttcgccaaac tttacggcct gtctcattct
     9241 tacgattgcg gcaggccgtg ttattattgt cgtttcttat attttgacag gaagagtatc
     9301 ttgactacta acactcatac tctgcagatt gaagagattt tagaacttct gccgcaccgt
     9361 ttccctttct tactggtgga tcgcgtgctg gattttgaag aaggtcgttt tctgcgcgca
     9421 gtaaaaaatg tctctgtcaa tgagccattc ttccagggcc atttccctgg aaaaccgatt
     9481 ttcccgggtg tgctgattct ggaagcaatg gcacaggcaa caggtattct ggcgtttaaa
     9541 agcgtaggaa aactggaacc gggtgagctg tactatttcg ctggtattga cgaagcgcgc
     9601 ttcaagcgcc cggtcgtgcc tggcgatcaa atgatcatgg aagtcacttt cgaaaaaacg
     9661 cgccgcggcc tgacccgttt taaaggggtt gctctggtcg acggtaaagt agtttgcgaa
     9721 gcaacgatga tgtgtgctcg tagccgggag gcctgatacg tgattgataa atccgccttt
     9781 gtgcatccaa ccgccattgt ggaagagggc gcgtcgattg gcgcgaacgc acacattggt
     9841 cctttttgta tcgttggacc ccatgtcgaa attggtgagg gtaccgtact gaaatctcac
     9901 gttgtcgtga atggccatac taaaattggc cgcgataatg agatttatca gttcgcctcc
     9961 atcggtgaag ttaaccagga tctgaaatat gctggcgaac cgacccgtgt ggaaatcggc
    10021 gatcgtaacc gcattcgcga aagcgtcacc attcatcgtg gcacagtcca gggcggtgga
    10081 ttgacgaagg tgggcagcga caacttactg atgatcaacg cgcacattgc gcacgattgt
    10141 acggtaggta accgctgtat tctcgccaac aacgcaacgc tggcgggtca cgtatcggta
    10201 gacgacttcg cgatcatcgg cggcatgacc gcagtccatc agttctgcat cattggtgcg
    10261 catgtgatgg ttggcggttg ctccggtgtg gcgcaggacg tccctcctta tgtcattgcg
    10321 cagggtaacc acgcaacgcc gttcggtgtc aatatcgaag ggctgaagcg ccgcggattc
    10381 agccgcgagg cgattaccgc tatccgcaat gcgtataagc tgatttatcg cagcggtaaa
    10441 acgctcgatg aagtgaaacc ggaaattgct gaactggcgg aaacgtatcc ggaagtgaaa
    10501 gcttttaccg atttctttgc acgctcaacg cgcggtctga ttcgttaatg actaaacagc
    10561 gtccattaac gattgccctg gtcgccggag aaacctccgg cgatatcctg ggggccggtt
    10621 taatccgcgc tctgaaagaa cgtgtgccca acgcccgctt tgttggcgtt gccgggccac
    10681 gaatgcaggc tgaaggctgc gaagcctggt acgaaatgga agaactggcg gtgatgggca
    10741 ttgttgaagt gctcggtcgt ctgcgtcgct tactgcatat tcgtgccgat ctgacaaagc
    10801 gttttggtga actaaagcca gatgtttttg ttggcattga tgcgcctgac ttcaatatta
    10861 ccctcgaagg taaccttaaa aagcagggta tcaaaaccat tcattacgtc agtccatccg
    10921 tctgggcgtg gcgacagaaa cgcgttttca aaataggcag agccaccgat ctggtgctcg
    10981 catttctgcc tttcgaaaaa gcgttttatg acaaatacaa tgtaccgtgc cgctttatcg
    11041 gtcataccat ggctgatgcc atgccgttag atccagataa aaatgccgcc cgtgatgtgc
    11101 tggggatccc tcacgatgcc cactgcctgg cgttgctacc agggagccgt ggtgcagaag
    11161 ttgaaatgct tagtgccgat ttcctgaaaa cggcccagct tttgcgccag acatatccgg
    11221 atctcgaaat cgtggtgcca ctggtgaatg ccaaacgccg cgagcagttt gaacgcatca
    11281 aagctgaagt cgcgccagac ctttcagttc atttgctgga tgggatgggc cgtgaggcga
    11341 tggtcgccag cgatgcggcg ctactggcgt cgggtacggc agccctggag tgtatgctgg
    11401 cgaaatgccc gatggtggtg ggatatcgca tgaagccttt taccttctgg ttggcgaagc
    11461 ggctggtgaa aactgattat gtctcgctgc caaatctgct ggcgggtaga gagttagtca
    11521 aagaattatt gcaggaagag tgtgagccgc aaaaactggc tgcggcgctg ttaccgctgt
    11581 tggcgaacgg gaaaaccagc cacgcgatgc acgatacctt ccgtgaactg catcagcaga
    11641 tccgctgcaa tgccgatgag caggcggcac aagccgttct ggagttagca caatgatcga
    11701 atttgtttat ccgcacacgc acctggttgc gggtgtggat gaagtcggac gcgggccgtt
    11761 agttggcgcg gtcgtcaccg ctgcggtgat cctcgacccg gcgcgcccga ttgccgggct
    11821 gaatgattcc aaaaagctga gcgaaaaacg ccgtctggcg ctctgtgaag agatcaaaga
    11881 gaaagcgttg agctggagtc tgggccgcgc ggaaccccac gaaatcgacg agctgaacat
    11941 tcttcatgcg accatgctgg cgatgcagcg tgccgtcgct gggctgcata ttgcgccgga
    12001 atatgtgttg attgatggta accgctgccc gaaattaccg atgcctgcga tggctgtggt
    12061 gaaaggcgat agccgcgtac cggaaatcag tgccgcgtct atcctggcga aagtgacgcg
    12121 tgacgccgaa atggcggcgc tggatattgt tttcccgcaa tatggttttg cccaacacaa
    12181 agggtatcca accgcttttc atctggaaaa actggctgaa tatggcgcga ctgaacatca
    12241 tcgtcgcagc tttgggcctg tcaaacgagc actgggactt gcgtcctgat tcttgtcgag
    12301 attaagtaaa ccggaatctg aagatgtctg aaccacgttt cgtacacctg cgggtgcaca
    12361 gcgactactc gatgatcgat ggcctggcca aaaccgcacc gctggtaaaa aaggcggcgg
    12421 cgttgggtat gccagcactg gcgatcaccg atttcaccaa cctttgcggt ctggtgaagt
    12481 tctacggagc gggacatggc gcagggatta agcctatcgt cggggcagat tttaacgtcc
    12541 agtgcgacct gctgggtgat gagttaaccc acctgacggt actggcggcg aacaataccg
    12601 gctatcagaa tctgacgttg ctgatctcaa aagcgtatca gcgtgggtac ggtgccgctg
    12661 ggccgatcat cgatcgcgac tggcttatcg aattaaatga agggttgatc cttctttccg
    12721 gcgggcgcat gggcgacgtc ggacgcagtc ttttgcgtgg taacagcgcg ctggtagatg
    12781 agtgtgtcgc gttttatgaa gaacacttcc cggatcgcta ttttctcgag ctgatccgca
    12841 ccggcaggcc ggatgaagaa agctatctgc acgcggcggt ggaactggcg gaagcgcgcg
    12901 gtttgcccgt cgtggcgacc aacgacgtgc gctttatcga cagcagcgac tttgacgcac
    12961 acgaaatccg cgtcgcgatc cacgacggct ttaccctcga cgatcctaaa cgcccgcgta
    13021 actattcgcc gcagcaatat atgcgtagcg aagaggagat gtgcgagctg tttgccgaca
    13081 tcccagaagc tcttgccaac accgttgaga tcgccaaacg ctgtaacgtg actgtgcgtc
    13141 ttggtgaata cttcctgccg cagttcccga ccggggacat gagcaccgaa gattatctgg
    13201 tcaagcgtgc aaaagaaggc ctggaagagc gtctggcctt tttattccct gatgaggaag
    13261 aacgtcttaa acgccgcccg gaatatgacg aacgtctgga gactgaactt caggttatca
    13321 accagatggg cttcccgggc tacttcctca tcgttatgga atttatccag tggtcgaaag
    13381 ataacggcgt accggtaggg ccaggccgtg gctccggtgc gggttcactg gtggcctacg
    13441 cgctgaaaat caccgacctc gatccgctgg aatttgacct gctgttcgaa cgtttcctta
    13501 acccggaacg tgtctccatg cctgacttcg acgttgactt ctgtatggag aaacgcgacc
    13561 aggttatcga gcacgtagcg gacatgtacg gtcgtgatgc ggtatcgcag atcatcacct
    13621 tcggtacaat ggcggcgaaa gcggtgatcc gcgacgtagg ccgcgtgctg gggcatccgt
    13681 acggctttgt cgatcgtatc tcgaaactga tcccgcccga tccggggatg acgctggcga
    13741 aagcgtttga agccgagccg cagctgccgg aaatctacga agcggatgaa gaagttaagg
    13801 cgctgatcga catggcgcgc aaactggaag gggtcacccg taacgccggt aagcacgccg
    13861 gtggggtggt tatcgcgccg accaaaatta ccgattttgc gccgctttac tgcgatgaag
    13921 agggcaaaca tccggtcacc cagtttgata aaagcgacgt tgaatacgcc ggactggtga
    13981 agttcgactt ccttggtttg cgtacgctca ccatcatcaa ctgggcgctg gagatgatca
    14041 acaagcggcg ggcgaagaat ggcgagccac cgctggatat cgccgcgatc ccgctggatg
    14101 acaagaaaag cttcgacatg ctgcaacgct cggaaaccac ggcggtattc cagcttgaat
    14161 cgcgcggcat gaaggacctg atcaagcgtc tgcaacctga ctgcttcgaa gatatgatcg
    14221 cactggtggc actgttccgc cctggtccgt tgcagtcagg gatggtggat aactttattg
    14281 accgtaaaca tggtcgcgaa gaaatctcct atccagacgt acagtggcag catgaaagcc
    14341 tgaaaccggt actggagcca acctacggca ttatcctgta tcaggaacag gtcatgcaga
    14401 ttgcccaggt gctttctggt tataccctcg gtggcgcgga tatgctgcgt cgagcgatgg
    14461 gtaagaaaaa gccggaagag atggctaagc agcgttctgt atttgctgaa ggtgcagaaa
    14521 agaacggaat caacgccgaa ctggcgatga aaatcttcga cctggtggag aaattcgcgg
    14581 gttacggatt taacaaatcg cactctgcgg cctatgcttt ggtgtcatat caaacgttat
    14641 ggctgaaagc gcactatccg gcggagttta tggcggcggt aatgaccgcc gatatggaca
    14701 acaccgagaa ggtggtgggc ctggtggatg agtgctggcg gatggggctg aaaatcctgc
    14761 caccagatat aaactccggt ctttaccatt tccacgtcaa cgacgacggc gaaatcgtgt
    14821 atggtattgg cgcgatcaaa ggggtcggtg aaggtccgat tgaggccatc atcgaagccc
    14881 gtaataaagg cggctacttc cgcgaactgt ttgatctctg cgcccgaacc gacaccaaaa
    14941 agttaaaccg gcgggtgctg gaaaaactga tcatgtccgg ggcgtttgac cgtcttggac
    15001 cacaccgcgc ggcactgatg aactcgctgg gcgatgcgtt aaaagcggca gatcaacacg
    15061 cgaaagcgga agctatcggt caggccgata tgttcggcgt gctggccgaa gagccggaac
    15121 aaattgaaca atcctacgcc agctgccaac cgtggccgga gcaggtggta ttagatgggg
    15181 aacgtgaaac gttaggcctg tacctgaccg gacaccctat caaccagtat ttaaaagaga
    15241 ttgagcgtta tgtcggaggc gtaaggctga aagacatgca cccgacagaa cgtggtaaag
    15301 tcatcacggc tgcggggctc gttgttgccg cgcgggttat ggtcaccaag cgcggcaatc
    15361 gtatcggtat ctgcacgctg gatgaccgtt ccgggcggct ggaagtgatg ttgtttactg
    15421 acgccctgga taaataccag caattgctgg aaaaagaccg catacttatc gtcagcggac
    15481 aggtcagctt tgatgacttc agcggtgggc ttaaaatgac cgctcgcgaa gtgatggata
    15541 ttgacgaagc ccgggaaaaa tatgctcgcg ggcttgctat ctcgctgacg gacaggcaaa
    15601 ttgatgacca gcttttaaac cgactccgtc agtctctgga accccaccgc tctgggacaa
    15661 ttccagtaca tctctactat cagagggcgg atgcacgcgc gcggttgcgt tttggcgcga
    15721 cgtggcgtgt ctctccgagc gatcgtttat taaacgatct ccgtggcctc attggttcgg
    15781 agcaggtgga actggagttt gactaataca ggaatactat gagtctgaat ttccttgatt
    15841 ttgaacagcc gattgcagag ctggaagcga aaatcgattc tctgactgcg gttagccgtc
    15901 aggatgagaa actggatatt aacatcgatg aagaagtgca tcgtctgcgt gaaaaaagcg
    15961 tagaactgac acgtaaaatc ttcgccgatc tcggtgcatg gcagattgcg caactggcac
    16021 gccatccaca gcgtccttat accctggatt acgttcgcct ggcatttgat gaatttgacg
    16081 aactggctgg cgaccgcgcg tatgcagacg ataaagctat cgtcggtggt atcgcccgtc
    16141 ttgacggtcg tccggtgatg atcattggtc atcaaaaagg tcgtgaaacc aaagaaaaaa
    16201 ttcgccgtaa ctttggtatg ccagcgccag aaggttaccg caaagcactg cgtctgatgc
    16261 aaatggctga acgctttaag atgcctatca tcacctttat cgacaccccg ggggcttatc
    16321 ctggcgtggg cgcagaagag cgtggtcagt ctgaagccat tgcacgcaac ctgcgtgaaa
    16381 tgtctcgcct cggcgtaccg gtagtttgta cggttatcgg tgaaggtggt tctggcggtg
    16441 cgctggcgat tggcgtgggc gataaagtga atatgctgca atacagcacc tattccgtta
    16501 tctcgccgga aggttgtgcg tccattctgt ggaagagcgc cgacaaagcg ccgctggcgg
    16561 ctgaagcgat gggtatcatc gctccgcgtc tgaaagagct gaaactgatc gactccatca
    16621 tcccggaacc gctgggcggc gctcaccgta acccggaagc gatggcggca tcgttgaaag
    16681 cgcaactgct ggcggatctg gccgatctcg atgtgttaag cactgaagat ttaaaaaatc
    16741 gtcgttatca gcgcctgatg agctacggtt acgcgtaatt cgcaaaagtt ctgaaaaagg
    16801 gtcacttcgg tggccctttt ttatcgccac ggtttgagca ggctatgatt aaggaaggat
    16861 tttccaggag gaacacatga acatcattgc cattatggga ccgcatggcg tcttttataa
    16921 agatgagccc atcaaagaac tggagtcggc gctggtggcg caaggctttc agattatctg
    16981 gccacaaaac agcgttgatt tgctgaaatt tatcgaacat aaccctcgaa tttgcggcgt
    17041 gatttttgac tgggatgagt acagtctcga tttatgcagc gatatcaatc aacttaatga
    17101 atatctcccg ctttatgcct tcatcaacac ccactcgacg atggatgtca gcgtgcagga
    17161 tatgcggatg gcgctctggt tttttgaata tgcgctgggg caggcggaag atatcgccat
    17221 tcgtatgcgt cagtacaccg acgaatatct tgataacatt acaccgccgt tcacgaaagc
    17281 cttgtttacc tacgttaaag agcggaagta caccttttgt acgccggggc atatgggcgg
    17341 caccgcatat caaaaaagcc cggttggctg cctgttttat gattttttcg gcgggaatac
    17401 cctcaaggct gatgtctcta tttcggtcac cgagcttggt tcgttgctcg accacaccgg
    17461 gccacacctg gaagcggaag agtacatcgc gcggactttt ggcgcggaac agagttatat
    17521 cgttaccaac ggaacatcga cgtcgaacaa aattgtgggt atgtacgccg cgccatccgg
    17581 cagtacgctg ttgatcgacc gcaattgtca taaatcgctg gcgcatctgt tgatgatgaa
    17641 cgatgtagtg ccagtctggc tgaaaccgac gcgtaatgcg ttggggattc ttggtgggat
    17701 cccgcgccgt gaatttactc gcgacagcat cgaagagaaa gtcgctgcca ccacgcaggc
    17761 acaatggccg gttcatgcgg tgattaccaa ctccacctat gatggcttgc tctacaacac
    17821 cgactggatc aaacagacgc tggatgtccc gtcgatccac ttcgattccg cctgggtgcc
    17881 gtacacccat tttcatccga tctaccaggg gaaaagtggt atgagcggcg agcgtgttgc
    17941 gggaaaagtg atcttcgaaa cgcaatcgac ccacaaaatg ctggcggcgt tatcgcaggc
    18001 ttcgctgatc cacattaaag gcgagtatga cgaagaggcg tttaacgaag cctttatgat
    18061 gcataccacc acctcgccca gttatcccat tgttgcttcg gttgagacgg cggcggcgat
    18121 gctgcgtggt aatccgggca aacggctgat taaccgttcg gtggagcggg cgctgcattt
    18181 tcgcaaagag gtccagcggt tgcgggaaga gtctgacggt tggtttttcg atatctggca
    18241 accgccgcag gtggatgaag ccgaatgctg gcccgttgcg ccaggcgaac agtggcacgg
    18301 ctttaacgat gcggatgccg atcatatgtt tctcgatccg gttaaagtca ctattttgac
    18361 accggggatg gacgaacagg gcaatatgag cgaggagggg atcccggcag cgctggtggc
    18421 aaaattcctc gacgaacgtg ggatcgtagt agagaaaacc ggcccttata acctgctgtt
    18481 tctctttagt attggtatcg ataaaaccaa agcaatggga ttattgcgtg ggttgacgga
    18541 atttaaacgc tcttacgatc tcaacctgcg gatcaaaaat atgctacccg atctctatgc
    18601 tgaagatccc gatttctacc gcaatatgcg tattcaggat ctggcgcaag ggatccataa
    18661 gctgattcgt aaacacgatc ttcccggttt gatgttgcgg gcattcgata ctttgccgga
    18721 aatgatcatg acgccacatc aggcatggca gagacagatt aaaggtgaag tagaaaccat
    18781 tgcgctggaa caactggtcg gtagagtatc ggcaaatatg atcctgcctt atccaccggg
    18841 cgtaccgctg ttgatgccag gagaaatgct gaccaaagag agccgcacgg tactcgattt
    18901 tctactgatg ctttgttccg tagggcaaca ttaccccggt tttgaaacgg atattcacgg
    18961 cgcgaaacag gacgaagacg gcgtttaccg cgtacgagtc ctaaaaatgg cgggataact
    19021 tgccagagcg gcttccgggc gagtaacgtg ctgttaacaa ataaaggaga cgttatgctg
    19081 ggtttaaaac aggttcacca tattgcgatt attgcgacgg attatgcggt gagcaaagct
    19141 ttctactgcg atattcttgg tttcacgctg caaagcgaag tctatcgcga agcgcgcgac
    19201 tcgtggaaag gggatttggc gcttaatggg caatatgtga ttgagctttt ctcttttccg
    19261 ttcccgccgg agcgccccag ccgaccggaa gcttgcggcc tgcgtcatct ggcttttagc
    19321 gttgatgata tcgatgcggc agtggcgcac cttgaaagcc ataacgtgaa gtgtgaagcc
    19381 atccgtgtcg atccatacac gcaaaaacgc ttcacgttct ttaacgatcc ggacgggctg
    19441 ccgttggaac tgtatgagca gtaaggcttg tcatcgccgc atttgcccgg taacgtgccg
    19501 ggcattgcta ctgtaaaatc gcaccatcat gacactcacg ctcaatagac aacttctcac
    19561 cttacgccag attctggtgg cctttagcgg cgggcttgac tccaccgttc tgctgcatca
    19621 gttggtgcag tggcggacgg aaaatccggg cgtcactcta cgcgctatcc atgtgcatca
    19681 cggtttaagt gccaatgccg atgcctgggt taggcattgc gagaacatct gccaacagtg
    19741 gcaggtgccg ctggtggtcg agcgcgtgca acttgcgcaa gaaggactgg gcattgaggc
    19801 ccaagcgcgg caggcgcgtt atcaggcatt tgcccgtacc ctattgcccg gcgaagtgct
    19861 ggtcaccgcg caacatctcg acgatcaatg tgaaaccttt ctgctggcgc taaaacgcgg
    19921 tagcggccct gccgggcttt cggctatggc ggaagtctcg gagtttgccg gaacacggct
    19981 tatccgcccg ttgcttgccc gcacgcgggg ggaactggcg cagtgggcat tggcgcatgg
    20041 tttacgctgg attgaagacg aaagtaatca ggacgactca tacgatcgta actttctgcg
    20101 cctgcgcgta gtgccgttat tgcagcagcg ttggccgcat tttgccgaag caacggcccg
    20161 cagcgccgca ctttgtgctg aacaagagag tctgttggat gaactgctgg cggatgattt
    20221 agcacactgc caaacgtcgc aggggacgct gcagattgcg ccaatgctag cgatgagtga
    20281 tgcccgtcgc gcggcgatta tccgccgctg gctggcaggg cagaatgcac cgatgccttc
    20341 ccgcgacgcg ctggtgcgga tctggcagga agtggcgctg gcgcgggaag atgcctcccc
    20401 ctgtttacgt tttggcgcgt ttgaaatacg gcgctatcag tcgcaactgt ggtggattaa
    20461 ctccgtcact gggcaaagcg aaaccattgt gccgtggcag acgtggctac aaccgctgga
    20521 attaccggcg gggctgggaa gtgtacagct tactgcggga ggcgatattc gccctccgcg
    20581 tgcagacgaa gcggtcagcg tgcgtttcaa agcgccagga ttgctgcata ttgtcgggcg
    20641 taacggcgga cgtaagctaa agaaaatctg gcaagagctg ggcgtgccgc cgtggctacg
    20701 tgacaccacg ccactgctgt tttatggcga aacgctgatt gcggcggcag gggtatttgt
    20761 gacgcaagaa ggtgtggctg aaggtgagaa tggcgtaagt tttgtctggc agaaaacgct
    20821 tagttaagtg aaagccggat aagacgcatc aaacgtcgca tccggcgaaa gtcaatcagg
    20881 actcgctcac caccaccgta ccgatttccg ggtggctaaa gctggtaatt ttatccagac
    20941 gtagctcacg ggtttcgcca gcggcctcga cgaccagata ctccacattt ttgcgggaga
    21001 ctaaatcgct ggcttttgcc tgcaattttt cgccatcttt cagctcaagt gtcagcatta
    21061 aatgatgctg gcaggcgagc tcaagattat cgtaatcatc acaattgatt ggttgatacg
    21121 tatcattcat tgacataatc gctcaccagt aagtttgccg cagcgtatgc tgctttttcc
    21181 ctgacagcct cagaaagggc gtcgtcggca gccatttcat tcagcacttt caaaacgcag
    21241 cccagcgcgt ccggaacgta tcctaagtct ccgctggcga tttccgcgta ccgcttgcgt
    21301 attaactcac aatatttttc cacatgccct cctgtcagca ctctgactta accgtggatg
    21361 caagtctaag cctacgaaga taaactctgt ttcgcaaggt gactatacca cactcatttc
    21421 tgcaatatca gcgccgcaac tgcacgtatt ccgttacaat ggcctcctga ttcgaaagga
    21481 gttttcttat ggcgcttaaa gcgacaattt ataaagcgac ggttaatgtg gccgatctcg
    21541 accgcaacca gtttctcgat gcctctctga cgctggcgcg ccacccttca gaaacccagg
    21601 agcgtatgat gctgcgcttg ctggcgtggc tgaaatatgc tgatgaacgt ctgcaattta
    21661 cccgtggttt gtgtgccgat gatgagccgg aagcgtggct gcgtaacgat cacctgggca
    21721 ttgatttgtg gattgagctg gggctgccgg atgagcggcg gattaagaaa gcctgcaccc
    21781 aggccgcaga agtggtgctg tttacctata atagtcgggc ggcgcaaatc tggtggcagc
    21841 aaaatcagag caaatgtgtg cagtttgcca atctttccgt ctggtatctg gacgatgaac
    21901 aactggcgaa agtaagcgcc tttgccgatc gtaccatgac gctgcaggca acgattcagg
    21961 atggcgtgat ctggttatcg gacgataaga ataatctgga agttaactta accgcctggc
    22021 aacaaccttc atgattgtga tttcccgaca tgttgctatt cccgatggtg agcttgagat
    22081 caccgccatt cgtgcgcagg gcgcgggcgg gcagcatgtt aataagacct caacggccat
    22141 tcatctgcgt tttgacattc gggcgtccag cctgccagag tattacaaag agcgtctact
    22201 cgccgccagc catcatttga tcagcagtga tggcgtgatt gtcattaagg cacaggaata
    22261 ccgcagtcag gaactgaacc gcgaagccgc gctagcccgg ctggtggcag tgattaaaga
    22321 tttaacaaca gaacaaaaag cccgacgacc cacgcggccc acccgtgcat cgaaagagcg
    22381 caggctggca tcgaaagcac aaaaatcaag cgtgaaggcg atgcgcggca aagtacgcag
    22441 cggtcgggaa taaaaagaag gaatagatgg tgaaaaaagc gatagtgaca gcgatggctg
    22501 taatcagcct ctttactctg atgggatgta ataatcgggc cgaagttgat gcgctttctc
    22561 cggcgcaggc tgccgaactg aaaccgatgc cgcaaagttg gcgcggcgtg ctgccgtgtg
    22621 ctgattgcga aggaatcgaa acctctctgt tcctcgaaaa agacggaaca tgggtgatga
    22681 atgagcgtta tctcggtgct cgtgaagaac cttcctcctt cgcttcctac ggtacatggg
    22741 cgcgaaccgc tgacaagctg gtattaaccg acagcaaagg tgaaaagtca tattatcggg
    22801 cgaaaggcga tgcgctggag atgctcgatc gtgaaggcaa tccgattgaa tcgcagttca
    22861 actatacgct ggaaccggca caatccagtt tacctatgac gccgatgacc ctgcgaggca
    22921 tgtattttta tatggctgat gcggcgacct tcactgattg cgcgaccgga aaacgtttca
    22981 tggtagcgaa taacgcagag ctggagcgta gctacctggc tgcgcgcggt cacagtgaaa
    23041 aaccggtgtt actgtcagta gaaggtcact ttacgcttga ggctaatccg gatactggtg
    23101 cgccaaccaa agtattagcg cccgatacgg cgggtaaatt ttacccgaat aaggattgca
    23161 gtagtttggg gcagtaaacc tgcttaaggc agaaacaaac gcaaaattgc ctgatgcgct
    23221 gcgcttatca ggcctacagc atataatgca acattttgaa ttagcacgat tttgtaggcc
    23281 ggataaggcg tttacgccgc atccggcatt ttgtgcacga ggctggcttt atcactgagt
    23341 cagcccaaca aagcgacccg ccttgatata aatcccaggc ttcaggtgcc caacatactg
    23401 taactgcgtt tctggcttaa acgcagagac atcaccaata cgcatatgca tcagatcggc
    23461 agggctaatc cagcccgatt gcgccagtgt aagtggatgc ccggctttgg caaaggcatc
    23521 cgtgacaaat tccgaacaaa accacgactt tttgtctcct tcgcccacac tgcttaactg
    23581 cgctttcgcc aggccgctga cgcactttgg cgaaaatcct cggagaacgg attcagtgag
    23641 cacatctggc gagtcaccat aaagggaata aattcgacaa tgccgcgata gttataaccg
    23701 ctatctttga ttttattggc aaacgcggtg atatctgtgg cttgttgcgg ggtaagatcc
    23761 gggactcgta agacgaaaag cttatcacta tgcttcatcg cttttttaag ggaaacaatc
    23821 tggacgccag cgcctgtcgc ttctgcaacg ttattatcac ccagaaagat tgcaacgtga
    23881 ctcacagagg aagtgctgaa gacgcggatt ccaaatgagg ttaccccaag gcttgaggag
    23941 aacagcaaat cgccgggttt gagatcaggt gccgttattt cttttattga ttgttcggtg
    24001 aaagagcttt gatgctggaa tttgacagcc catgtttttg cctcggcatc tactgctgtg
    24061 gctgatgggt ctggctggct gatatcaacg gtacaagccg aaagtaaaag aaagctgggg
    24121 agaaacaggc ggcagtacgc ctttggttta tccattttat acaatccatg taaaaaaggg
    24181 ccctgaaatt caggaccctt tctggcatca gcctttaatc tgtttcacca gatattcgac
    24241 gatgtcacca gtcttaatta actgtttctc gccgttgcga cgatatttat attcgatatc
    24301 gtcgttgtcg aggttacggt cgcccagcac aatagtgtgc ggaataccga tcagttccat
    24361 atcggcaaac atcacgcccg gacgttcttt tcggtcatcc agcagcactt cgataccttg
    24421 tgcacgcagt tcgctgtaca gtttctcagc aagctcttgt acgcggaagg atttgtgcat
    24481 gttcatcggc agaatcgcca cctggaacgg cgcgatagcg tcaggccata cgatgccgcg
    24541 ttcgtcgtag ttctgctcaa tcgccgcagc taccacacgc gttaccccga taccgtagca
    24601 acccatcgtc aggatttggt tacggccatc ttcaccctgt acggaggctt tcagtgcttc
    24661 ggagtacttg gtacccagct ggaagatgtg accaacttcg ataccacgtt tgatcagcag
    24721 cgtaccctgg ccatccgggc ttggatcgcc cgccaccacg ttgcggatat cagcaatttc
    24781 cggggtagcg acatcgcgat cccagttgat gccgaagtag tgtttaccat cgatgttagc
    24841 accagcagcg aaatcactca tcgccgcaac ggtacggtca atcaccaccg gaatcggcat
    24901 gtttaccgga cccagtgaac ccggaccggc gttaaccacg gcacgaattt cttcttcggt
    24961 cgcgaaagtc agcgggctgg caacctgcgg cagtttttct gctttaactt cgttcagttc
    25021 gtggtcaccg cgcaccagca gcgcaaccag cgggaagcta ctgccttcaa ccgctttaac
    25081 cagcagagtc ttaaccgttt tctcaatcgg cagattgaac tgttcaacca gttccgcgat
    25141 ggttttcgcg ttcggcgtat caaccagcgt catttcctgg gtagcagcag cgcgcggttc
    25201 tttcggcgcg atagcttctg ccagttcaat gttcgctgca tagtcagagg tgtcggagaa
    25261 gaccacatcg tcttcaccgc tctgcgccag cacctggaat tcgtgagagg cgctgccgcc
    25321 gatagaaccg gtgtcggcct gtacggcgcg gaaatccagc cccatgcggc tgaagatttt
    25381 gctgtaggcc gcatacattg catcgtaggt ttcctgcagg gattcctgag aagtatggaa
    25441 agagtaagca tctttcatca ggaattcgcg ggaacgcatg acgccgaaac gcggacgcac
    25501 ttcgtcgcgg aacttggtct ggatctgata gaagttcagc ggcagctgtt tgtaagagct
    25561 aagctcgtta cgaatcaggt cagtgataac ttcttcatga gttgggccga gtacgaacgg
    25621 acgctcgcca cggtcaacaa aacgcagcag ttccggaccg tactgttccc aacgaccact
    25681 ctcttgccac aaatcggctg gctgaaccac cggcatcgac acctcgatcg caccggcgtt
    25741 gttcatctct tcacgcacga tgttttcgac ttttttcaga acgcgcacgc cggtcggcag
    25801 ccaggtatat aacccggagg ccagcttgcg gatcatcccg gcgcgcagca tcagctgatg
    25861 gctgatcacc tcggcgtcgg caggtgtctc cttgagagtg gagagcaggt attggctagt
    25921 acgcatgttg ttacggttcc agttggaagg tagaacaggc tcaaggcgag cctgggacaa
    25981 aaaaaagtga tttagtttac cagtgcaaaa gaaatgtcaa aagagaaggg cgtgaattta
    26041 acgcggttcc agcgcaaaga cttcaaaacc tgcgtcggtg acgcgccagc gaacgttaaa
    26101 atcatgtagc cagacggcat aggttttgcc cgtttcctca cctttacgat aggccgggcg
    26161 cgggtcctgc gccagtactt cgcggataaa cagcgttaac tgcggataac gcttctccag
    26221 cgtcaaaagc tgcttttcga cctctgcggt aaaactcacc gccatctctg ccgctggcgc
    26281 actttgcgca tagctggcac tggcatcggg aagcgattcg gcaaagggga gatacggttt
    26341 gatatccacc accggcgtac catcgaccag atccagactg ccgagcttca gaatcacgct
    26401 gtctttatgg caaacaacct ctttcagctc taccagcgac ataccaattg ggttagggcg
    26461 gaaagtagag cgtgttgcga aaacccccat tctggcgtta ccgccgaggc gcggtggacg
    26521 cacagtcgga cgccagccgc cttccatcgt ttgatgaaag acgaaaagga tccataaatg
    26581 gctgaacgct tccaggccgc gaacggcgtc ggcctggttg tagggagcaa tgagatgcag
    26641 ttcgccgttg gcgcttttta ccagacctgg ctggcgcgga acggcgaatt tttctttata
    26701 gggcgagcga ataacgccta tttgctcgaa ctggaaactg ctcatttcgc cgtaatgtta
    26761 agcgcagagc cgatacatac agcctgacga tagcagcctg gcgtaccgct ggtgacttcg
    26821 cagctatgca gtaataccgc attggccttc attttcgagg cgttgatttg catccgctta
    26881 cgtgcggttg gaatgctcgg cggagagtcc tgattggagg cctggcaaga atcgccactg
    26941 acttcaccga gatcgcggaa cggtttgccg actaattctt ctgcattggt ataaattcgg
    27001 accggcgtgg cgcgcggcgc tttcggtttt gcaggctccg ctttcggctg gggtgcagtg
    27061 ctttgaacgg gttcgacagg ggatctgctt aacatggaac agccgcttag catgagtgct
    27121 actaaacaga tcggtaaagc acgcatagta tttcctcaat gtatgatcaa aacgtcaata
    27181 ttgaatcagg agcttgtaaa aatgacaaga cgggcaagcg cccgtcctga atgatattac
    27241 aaattgtgga aacagcctaa aaattaccag cctttaacag cgccgccgtt aaacactttg
    27301 tttgctgctt cgtaaacttc atcagactga taagcctgga cgaatttctt cacgttctcg
    27361 gcgtctttgt tatcttcacg cgttacgatc aggtttacgt acggggagtc tttatcttcg
    27421 acaaagatac cgtctttcgc tggagtcagg ccaatctggc tggcataggt ggtattgata
    27481 actgccagag cgatttgcgc gtcgtccaga gatcgaggca gctgcggtgc ttccagctca
    27541 acaattttca gattttttgg gttctcaaca acatcaagaa cggtcggcag caggccaacg
    27601 ccatctttca gtttgatcaa gcccactttt tgcagcagca gcagtgaacg accaagatta
    27661 gttggatcgt ttggcacggc aacctgcgaa ccatcctgca gttcatccag tgatttgatt
    27721 ttcttggagt aaccagcaat cggataaaca aaagtgttgc ctactgcgac cagtttgtag
    27781 ccacgatctt tcagttgctg atcaaggtac ggtttatgct ggaaggcgtt ggcgtcgata
    27841 tcgcctttgc tcaatgcttc gtttggcaga acatagtcgt tgaaggttac cagctcaacg
    27901 tccaggccat atttgtcttt cgcaactttc tgcgcgactt ctgctacctg ctgttcggca
    27961 ccaacaatca cgccgacttt aatgtggttt ggatcttttt catcctgacc gcagcctacc
    28021 agtgccagtg atccaatcag ggctcccact gccgcaaagg ttttgaattt gaacgccata
    28081 ccttattcct tcttcaatta tttatgttgt gttgaacgtt acttgcgagt gacagcccgg
    28141 acgatgcggt cgcctgcgaa ctgaattaaa taaaccagaa tgaccagcaa taccagtacc
    28201 gtattcatca ccgtcgcgtt atagccgatg tagccatact gatagccaat ctgacctaaa
    28261 ccaccggcac cgactgcacc acccatcgcg gaatagccga ccagggtaat cagggtgata
    28321 gttgccgcat tcaccagacc cggcagtgct tccggtaaca gcaccttacg gacgatctgc
    28381 atcggcgtgg cacccattgc gcgggaagct tcaattaacc cggttgggat ctccagcaga
    28441 gcgttctcga ccatacgggc aataaacggt gctgcaccaa cggttaacgg aacaatcgct
    28501 gcctgcaaac caatcgatgt accgacaata acgcgggtaa acggaatcat ccataccagc
    28561 aagataatga acgggatgga acgaaaaatg ttcacaatcg cagaaacggt acgatacagc
    28621 ttcgcgttag caataatttg ccccgggcgc gtaacataaa gcaggacgcc gaccggcaga
    28681 ccaatcacaa agccaaaaaa tccggatacg aaggtcattg ccagcgtttc ccatacgcca
    28741 cgaaccagca accacatcat cggctcagac ataacccagt acctctactt ttacatggtg
    28801 ttcctgcagc caggcaatgg cggcttgcgt atcttgttgt gtgccgtgca tttcagtcag
    28861 catgatgccg aacttaacgc caccggcgta atccatctgc gcgctaataa tgttgttgtt
    28921 gacgttgaaa cgacgcgcgg tttcagaaag cagtggggca tcgaccgact gaccggtaaa
    28981 ctccagacgc agcatcggca cgcagtcagt gaatggctcc gcttgcagac gttcctggta
    29041 atcttccggg atatccagat gcagggtcga ctgaataaac ttctgcgcca acggcgtttt
    29101 cggatgcgaa aacacttcac ttaccgtgtc ctgctcgatc agttcgccat tgctgatgac
    29161 cgccacgcaa tcacaaatac gctttacaac gtccatttcg tgggtgatca acagaatcgt
    29221 caaacccaga cggcggttaa tgtctttcag cagctcgaga atagaacgtg tcgttgccgg
    29281 gtccagcgcg ctggtggctt catcacacag caatactttg ggattgctgg ctaacgcacg
    29341 ggcaatcgcc acacgctgtt tctgcccacc ggaaagattc gatgggtagc tatcatgctt
    29401 atcgccaaga ccaaccaatg acagcaattc cgtcacgcga cgtttgatct cgtctttcgg
    29461 tgtgttgtcc agctccagcg gcagagccac gttgccaaaa acagtacgcg aagagagcag
    29521 gttaaaatgc tggaaaatca taccaatctg gcggcgagct ttggtcaact cggattctga
    29581 cagcgtggtc agttcctggc catcgaccag cacgctaccc tcggttgggc gctccagcag
    29641 gtttacacaa cgtataagcg tactcttacc cgcgcctgag gcaccgataa cgccataaat
    29701 ttgtccagct ggcacatgca ggctgacgtt gttcaacgcc tggatggtgc gggtgccctg
    29761 gtggaacact ttggtgatat tcgaaagttt tatcattgat tatttattat cgtcattaag
    29821 ttagtcgtgg catctcgaat gcctgaaacg ggcaacgccg tcaatgaaat ggatgttaag
    29881 gcatccagac gtctaaatca atcaggttta tgcgaagagc actttcttgc aggtcgaaac
    29941 atgcgatact agcgtcacat gccttattaa ggagctataa aaggtggcaa agagcgtacc
    30001 cgcaattttt cttgaccgtg atggcaccat taatgtcgat cacggctatg tccatgagat
    30061 cgacaacttt gaatttatcg acggtgttat tgacgccatg cgcgagctaa aaaaaatggg
    30121 ctttgcgctg gtggtagtaa ccaaccagtc tggcattgct cgcggtaaat ttaccgaagc
    30181 acagtttgaa acgctgaccg agtggatgga ctggtcgctg gcggaccgag atgtcgatct
    30241 ggatggtatc tattattgcc cgcatcatcc gcagggtagt gttgaagagt ttcgccaagt
    30301 ctgcgattgc cgcaaaccac atccggggat gcttttgtca gcacgcgatt atttgcatat
    30361 tgatatggcc gcttcttata tggtgggcga taaattagaa gatatgcagg cagcggttgc
    30421 ggcgaacgtg ggaacaaaag tgctggtgcg tacgggtaaa cctattacac ctgaagcaga
    30481 aaacgcggca gattgggtgt taaatagcct ggcagacctg ccgcaagcga taaaaaagca
    30541 gcaaaaaccg gcgcaatgat taaaagatga gcggttgaaa taaaaatgca t
//
LOCUS       DAMZET010000037        30347 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.037, whole genome shotgun sequence.
ACCESSION   DAMZET010000037 DAMZET010000000
VERSION     DAMZET010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.037"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..230
                     /locus_tag="POB34_001778"
     CDS             <1..230
                     /locus_tag="POB34_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein rhsA"
                     /protein_id="HDI5918746.1"
                     /translation="DITMIVSRGNVADTGITDRVNDIINDRFWSDGKKPDRCDVLQEL
                     IDCGDISAKDAKSTQKAWNCRHSRQSNDKKR"
     gene            251..1093
                     /locus_tag="POB34_001779"
     CDS             251..1093
                     /locus_tag="POB34_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HDI5918747.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKKLNDDELFRLLDDHNSLKRISSA
                     RVLQLRGGQDAVRLAIEFCSDKNYIRRDIGAFILGQIKICKKCEDNVFNILNNMALND
                     KSACVRATAIESTAQRCKKNPIYSPKIVEQSQITAFDKSTNVRRATAFAISVINDKAT
                     IPLLINLLKDPNGDVRNWAAFAININKYDNSDIRDCFVEMLQDKNEEVRIEAIIGLSY
                     RKDKRVLSVLCDELKKNTVYDDIIEAAGELGDKTLLPVLDTMLYKFDDNEIITSAIDK
                     LKRS"
     gene            1121..2079
                     /locus_tag="POB34_001780"
                     /pseudo
     CDS             1121..2079
                     /locus_tag="POB34_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            2091..2552
                     /locus_tag="POB34_001781"
     CDS             2091..2552
                     /locus_tag="POB34_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDI5918748.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
     gene            3259..3696
                     /locus_tag="POB34_001782"
     CDS             3259..3696
                     /locus_tag="POB34_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918749.1"
                     /translation="MADKAILWALISASTKEGRKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADCIETVNK
                     IKDKNKKIIDINSVNICSDDKNIKLRVNSTIMAIDDSIKCIDE"
     gene            complement(4156..5292)
                     /locus_tag="POB34_001783"
     CDS             complement(4156..5292)
                     /locus_tag="POB34_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDI5918750.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5295..5657)
                     /locus_tag="POB34_001784"
     CDS             complement(5295..5657)
                     /locus_tag="POB34_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDI5918751.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            6194..8107
                     /gene="mtlA"
                     /locus_tag="POB34_001785"
     CDS             6194..8107
                     /gene="mtlA"
                     /locus_tag="POB34_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDI5918752.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            8337..9485
                     /gene="mtlD"
                     /locus_tag="POB34_001786"
     CDS             8337..9485
                     /gene="mtlD"
                     /locus_tag="POB34_001786"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDI5918753.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGTLPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            9485..10072
                     /gene="mtlR"
                     /locus_tag="POB34_001787"
     CDS             9485..10072
                     /gene="mtlR"
                     /locus_tag="POB34_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HDI5918754.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(10084..10293)
                     /locus_tag="POB34_001788"
     CDS             complement(10084..10293)
                     /locus_tag="POB34_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918755.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            10578..10940
                     /locus_tag="POB34_001789"
     CDS             10578..10940
                     /locus_tag="POB34_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HDI5918756.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            11483..12166
                     /locus_tag="POB34_001790"
     CDS             11483..12166
                     /locus_tag="POB34_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDI5918757.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            12210..17060
                     /gene="ehaG"
                     /locus_tag="POB34_001791"
     CDS             12210..17060
                     /gene="ehaG"
                     /locus_tag="POB34_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HDI5918758.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPSKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLDNRVTNLDSRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDASAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            17428..19083
                     /gene="lldP"
                     /locus_tag="POB34_001792"
     CDS             17428..19083
                     /gene="lldP"
                     /locus_tag="POB34_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDI5918759.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            19083..19859
                     /gene="lldR"
                     /locus_tag="POB34_001793"
     CDS             19083..19859
                     /gene="lldR"
                     /locus_tag="POB34_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HDI5918760.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            19856..21046
                     /gene="lldD"
                     /locus_tag="POB34_001794"
     CDS             19856..21046
                     /gene="lldD"
                     /locus_tag="POB34_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HDI5918761.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            21232..21705
                     /gene="trmL"
                     /locus_tag="POB34_001795"
     CDS             21232..21705
                     /gene="trmL"
                     /locus_tag="POB34_001795"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDI5918762.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(21758..22579)
                     /gene="cysE"
                     /locus_tag="POB34_001796"
     CDS             complement(21758..22579)
                     /gene="cysE"
                     /locus_tag="POB34_001796"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDI5918763.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(22659..23678)
                     /gene="gpsA"
                     /locus_tag="POB34_001797"
     CDS             complement(22659..23678)
                     /gene="gpsA"
                     /locus_tag="POB34_001797"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDI5918764.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(23678..24145)
                     /gene="secB"
                     /locus_tag="POB34_001798"
     CDS             complement(23678..24145)
                     /gene="secB"
                     /locus_tag="POB34_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HDI5918765.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(24208..24459)
                     /gene="grxC"
                     /locus_tag="POB34_001799"
     CDS             complement(24208..24459)
                     /gene="grxC"
                     /locus_tag="POB34_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HDI5918766.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(24601..25032)
                     /locus_tag="POB34_001800"
     CDS             complement(24601..25032)
                     /locus_tag="POB34_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDI5918767.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCEEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            25277..26821
                     /gene="gpmM"
                     /locus_tag="POB34_001801"
                     /gene_synonym="pgmI"
     CDS             25277..26821
                     /gene="gpmM"
                     /locus_tag="POB34_001801"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDI5918768.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            26831..28114
                     /gene="envC"
                     /locus_tag="POB34_001802"
     CDS             26831..28114
                     /gene="envC"
                     /locus_tag="POB34_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HDI5918769.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            28118..29077
                     /locus_tag="POB34_001803"
     CDS             28118..29077
                     /locus_tag="POB34_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HDI5918770.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(29064..30098)
                     /gene="waaH"
                     /locus_tag="POB34_001804"
     CDS             complement(29064..30098)
                     /gene="waaH"
                     /locus_tag="POB34_001804"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HDI5918771.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
BASE COUNT         8021 a         7385 c         7617 g         7324 t
ORIGIN      
        1 tagatataac aatgatagtt tctcgtggaa atgttgcaga cacagggata actgatcgtg
       61 tcaatgacat aataaatgac agattctgga gtgatgggaa aaaacccgac agatgtgacg
      121 tacttcagga actaattgat tgtggtgata ttagtgctaa agatgcaaaa agcacacaga
      181 aagcctggaa ttgtcgtcac tccagacagt caaacgataa aaaaagatag cccttgtgga
      241 ggttcctgca atgtcaaata cataccagaa aagaaaggca agtaaagagt atggtttata
      301 taatcaatgt aagaaactaa atgatgatga attatttcgc ttacttgatg atcacaattc
      361 cttgaaaagg atttcatctg ccagagtatt acagttaaga ggtgggcaag acgctgttag
      421 attggcaatt gagttctgct ctgataaaaa ttatatccgt agagatatcg gagcatttat
      481 actcgggcaa ataaaaattt gcaaaaaatg cgaagataat gtttttaata ttttgaacaa
      541 tatggcattg aatgataaga gcgcttgcgt tcgagctacg gcaatcgagt caacggctca
      601 gcgatgcaag aaaaacccaa tttattcacc taaaatagta gaacaatctc aaattactgc
      661 ttttgataaa tcgactaatg tcagacgtgc tacagcattt gctatttctg ttatcaatga
      721 taaagcaaca attccactat tgattaatct gttaaaagat ccaaatggag atgtcagaaa
      781 ctgggccgca tttgcaataa atatcaataa atatgataat agtgatatta gggattgttt
      841 tgtggagatg cttcaggata aaaatgagga agtccgtatt gaagcaataa tcggactttc
      901 ctacagaaaa gataaaaggg ttttatctgt tttatgcgat gagttaaaaa aaaatactgt
      961 ttatgatgat atcattgaag ctgcgggtga attaggtgat aaaacgctac ttcctgtttt
     1021 agatactatg ttgtacaagt ttgatgacaa tgaaattata acttccgcta ttgataagct
     1081 gaagcgttca tagcgtgagt tgcctatgca cagtggggga ttcccgccgg cacggtgcag
     1141 atgctcgacc ggctggaaag tgaaatcctg gctgaccggg tgagtgagga aagccgccgc
     1201 tggctggcat cgtgcggcct gaccgtggag cagatgaaaa accagatgga cccggtgtac
     1261 acgccggcgc gaaaaatcca cctgtaccac tgcgaccatc gcggcctgcc gctggcgctc
     1321 atcagcacgg aaggggcaac agcgtggtgc gcagaatatg atgaatgggg caacctgctg
     1381 agtgatgaga acccgcatca tctgcagcag ctcattcgcc tgccgggtca gcagtatgat
     1441 gaggagtccg gcctgtatta caaccgccac cgctattatg acccgctgca ggggcggtat
     1501 atcactcagg atccgattgg gctgaagggg ggatggaatt tttatcagta tccgttgaat
     1561 ccggtcataa atgtagatcc gcaaggtttg gttgatataa atttataccc cgaaagtgat
     1621 cttatccatt ctgtagctga tgagattaat atcccaggcg ttttcacaat cggggggcat
     1681 ggtaccccca catctattga atccgcaacg cgcagtatca tgacagctaa agatctagca
     1741 tatctaatta aatttgatgg gaattataaa gatgggatga cagtttggtt attttcttgt
     1801 aatacaggta aaggacaaaa ttcatttgct agccaattag ctaaagagtt acatacaaat
     1861 gtaataggac ctgacacgct atggacgtgg tgggggcgag gaactaatgg taagttaaaa
     1921 atggatacag tgctaacagc accaacgaac cttaattcaa ataaggatct aatggctata
     1981 acaacaaaag accttggtaa ttggataaca tatgggccat ctgggcaccc catttctaat
     2041 atgcaaggta cgccagaaaa acccagtgat ataagatagg ttgtagatgt atgaaagcat
     2101 gcttgttact atttttttat ttttctctta ttggtcaatt gcatggtgct gatgtgaaaa
     2161 taaaagaaaa cgaaagtgtg atgggatcta cagcaatgac ctatgattta agtgaagaaa
     2221 agctgatgaa actgaagtat aaatcacaac atggtgattc agaggcatca tttcgactat
     2281 atcaatatta ctgctttact aaaaataata ttgataaaca attgcgattc ttggaaagat
     2341 cagcatctca ggggaatgtt acagcgcagt ttaattatgg ggtcttttta tcagatacaa
     2401 atccaacatt atcagaatat tataatttga atagagctat ttattggatg gaattcgctg
     2461 taaataacgg taatattgat gcaaagagca aacttcaaga gctcaaaaag ctaaagcgaa
     2521 tggatagaag gaagaataag gagaatccgt aaaagcaata gttcatcaga ctgccgcagc
     2581 agtagtatga tgatgaatca gggctgtact ataactatta tcggtaacat taaccgcagc
     2641 aggggcggta taacactcag gctccgattg ggctggaagg taggtggaat ttttatcagt
     2701 atccgttgaa cccgatttct ggaatcgatc cccttgggat tagccacctg tttatacagt
     2761 ataactttag gtatgctttc gtgtgtttca gatataccat gtgatgataa tttttatgat
     2821 gtgcttagta ttccagtcgc ttctggtaat aatatgcaat gtaaaaataa tccccgttgt
     2881 acacatttat aaatagatgg ccaatacctc aaggcctatg gagttggaat gttaatgacc
     2941 cgggagcaag caatagaaaa cctgacggca tacgattagt accgtcagtt aatacaggaa
     3001 cttataatcg caatggattt tcaatacata gttgtttaaa cgcttttggt ccgtctttag
     3061 gacctcgatt ctgttccgag ggttgtataa ctggtttatc taatgacatg caaaaattaa
     3121 atgagttaat attttcagag ccagatagca cattaacagt tacggactaa aaaatatggc
     3181 aaggttatta acaattgtat ttttttctct atttctgcga gtgcagttac tgcaagacaa
     3241 tttcagagag agttagattt ggcagacaaa gcaatattat gggcactaat atcagccagt
     3301 actaaagagg gacgaaaagc gtgttcacta agctattttg cctgtaaggc cgcagaggct
     3361 gaactcggac tggcatatat ggctgcgaat gataacaagg aatttctcac atccttatct
     3421 aatataatga ggtacaaaat agatgctgga ctttccgaat cctatacatg ctatttatta
     3481 agtaagggaa agatcataag accatatctg aaaaatctaa atccactcca acttgccgct
     3541 gattgcattg aaacagtaaa taaaataaaa gataagaata aaaaaatcat tgatataaat
     3601 tcagttaata tttgtagtga tgataaaaat ataaaattga gagttaatag tactattatg
     3661 gccattgatg attctataaa gtgcatcgat gaatgataac ttaattttat ttaaaaatat
     3721 attaataact cactctccag gagttttttt aacttagttt tataatgaat acgataatta
     3781 aaagtgaaga agaaaatagt tgtatacata tattccatga ccctacattg tgatctatag
     3841 attaaatatt ttttaaatgt gactgtcatc cttcagataa taaagccatc atcgcaatag
     3901 atggaaaagc actcctgctc ttttttgatt agagccgtcg caggaggaac aactcatatc
     3961 atcattgcga tctcgacgat gtatagtctc atcctgcggc agaacaagac ggataaaaaa
     4021 tccaatgaga gcacactcat ccagaactta ttaacataat gaatattaaa gaaaaatcat
     4081 cgcagctaat gaagtaatat gcaaaaataa ttagtcctta caaggagcaa agttccataa
     4141 tctcccccct ccccctcaat gatccaaata aagataatgc atccagctgg tcattcttag
     4201 cagcactttc cgcatcaccg aaacatggct gaaatggtcg gagtaaaccg ccacctgggc
     4261 gtagatgccg tcgggtaagg catcgatatc atcgttaggg tccagttcaa tggttcccag
     4321 cacaccgtcc gtgccgggca cgaccgttaa tgattgcaat accccctgcg cctgataaga
     4381 accgcctggc acgacaggta aaatactagt cagtttgccg tgaaacacct gcccaggtag
     4441 cgcgttaaac accacttccg catcatcgcc gggtttcaga cgtaacagcg agttttgccg
     4501 aaattgggcg acaatttgcc gtttttgctc ggggatgaag accatcaccg gacgcagcgg
     4561 caaggcagct gcgtatgtac ctgggcggat cagtacctga gtaacgtagc cattgctcgg
     4621 cgcgcgaatg acagtctgct caaggttata ttttgcttca gtaagttgcg ctcttaagct
     4681 cacaatctgc gattgctcgc cgttaaccat actgtcgagc tggctctgga tctgcgcctg
     4741 ctccgccacc gagcctttca ccagcgcatc ctgcgcgagg aaattttgcc gcgcatcgtc
     4801 gatgtcacgt tccgagaacg gattcaccgc cgcctggctg cctttcaagt aacgttgata
     4861 atttttaaac agacggtcgc gctccgctga aacctgggtg gtgttggcct gcgcttcagt
     4921 gagctgcgca cgcagcgtct ttatattatg cgtcgccgtc atcaggtcag cctgaagtct
     4981 gtcaactcgc gcctggtaac gaaccgggtc gagcttaaaa agcacctcgc ccttttgaat
     5041 aagctgatta ttcttgtcag tgacttcagt aacaattccc gtcacctgtg gcgtgatagg
     5101 gatcgctatc actgcctttt gcgcggtaaa agtgtaaggg tggttgtagt tcatcaacaa
     5161 aattaaacca ctcaccagaa acacgcctcc cagcgccgcc gtcgccagcg tccactgatt
     5221 taccggaatg cggaagattt taaagaccgc ccacgccagc gccacgtaag ttaaaacaat
     5281 caatagatcc ataattagat ctccgggaac gtggaatagt cagtcttttt ctcggcggcg
     5341 agttgatgct ccagccggga aattcggtca gaaagtgagg cgatttccgg gtcagtcgct
     5401 ttctcctgcg acgcaacatg tgactgcata ccccagccac gctccggttg atagagcgtc
     5461 gcccagatcc acagaaacgg ccagataaca tgcagagtaa acaggctcac ccagcccgcc
     5521 gtatgaatag cgtcggcatg ggggtgattg cgctttttgg caatcagata agggatgtca
     5581 tgtatcgcga tgattccata aaaaatcacc agaaagacga agatcagcac tcccaacgcg
     5641 aaatagttta gaaacatatc tgcctcggat tcacgtttat cagtgttgtt tttgggctgg
     5701 cagccagaag ggagtcaggc tgatattttg acaataatcc gggttcgcga ttctcgccat
     5761 aacaccaaag aataattttt agaggtgatg agttgcttag ttacataacg attgtatgac
     5821 gaaggcataa catgctgtag atcacatcag gtgaacgccg taagaaaata tcttgtgatt
     5881 cagatcacaa agattcaaca aaccattaaa acaaaaatgt gacactactc acatttaaat
     5941 gccattttta gcgaaaatcg ccgtcttgtt gcttttttac acaagcgttt tgtgatgaac
     6001 gtcacgtcaa ttacctctct accccctata tttatgtgat tgatatcaca caaaaggccg
     6061 tcgactggac agttaaccga ttcagtgcca gatttcgcag tatctacaag gtccggctac
     6121 ctctgccgcc acattaacaa aaaacctcgg gcttccagcc tgcgcgacag caaacataag
     6181 aaggggtgtt tttatgtcat ccgatattaa gatcaaagtg caaagctttg gtcgtttcct
     6241 cagcaacatg gtgatgccaa atatcggcgc gtttatcgcg tggggtatca tcaccgcgtt
     6301 atttattcca acagggtggt taccgaacga gacgctggcg aagctggtcg ggccgatgat
     6361 cacttatctc ctgccgctgc tgatcggtta taccggtggt aagctggtag gcggcgaacg
     6421 tggcggcgta gtcggtgcca tcactaccat gggcgttatc gtcggcgcag acatgccgat
     6481 gttcctcggt tctatgattg caggtccgct gggcggctgg tgcattaagc acttcgaccg
     6541 ctgggtagac ggtaagatca aatccggttt tgagatgctg gtgaataact tctccgcagg
     6601 catcatcggg atgatcctcg ccattctggc atttctcggc attggcccga ttgttgaagc
     6661 cctgtccaaa atgctggctg cgggcgttaa cttcatggtt gtccatgaca tgctgccgct
     6721 ggcgtctatc tttgttgaac cggcgaaaat cctgttcctc aacaacgcca ttaaccacgg
     6781 tatcttctcg ccgctgggta ttcagcagtc ccatgaactg ggtaaatcaa tcttcttcct
     6841 gattgaagct aacccaggtc caggtatggg cgtgctgctg gcgtacatgt tctttggtcg
     6901 tggtagcgct aaacagtctg cgggcggtgc ggcaatcatc cacttcctgg ggggtatcca
     6961 cgaaatctac ttcccgtatg tgctgatgaa tccgcgtctg atcctcgcag tcatcctcgg
     7021 cggtatgact ggcgtgttca cgctgactat cctgggcggt ggtctggttt ctccggcatc
     7081 tccgggttct atccttgctg tactggcgat gacaccaaaa ggtgcttatt tcgctaacat
     7141 cgcgggtgtg tgtgcggcga tggctgtctc cttcgttgtc tctgctattt tgctgaaaac
     7201 cagcaaagtg aaagaagaag atgatattga agcagcaact cgtcgtatgc aggacatgaa
     7261 agctgagtct aaaggcacat ctccgctgtc tgcgggcgat gtgactaacg acctgagcca
     7321 cgtacgtaaa atcatcgttg cctgtgacgc cggtatgggt tcaagtgcga tgggcgcagg
     7381 cgtgctgcgt aagaaaattc aggatgcagg tctgtcacag atttcggtca ctaacagcgc
     7441 gatcaacaac ctgccgccag atgtggacct cgtcatcact caccgtgacc tgaccgaacg
     7501 cgctatgcgc caggttccgc aggcgcagca tatttcgctg accaacttcc tcgacagcgg
     7561 cctgtacacc agcctaaccg aacgtctcgt tgctgcccag cgccatactg aaaacgaagt
     7621 gaaagtgaaa gacagtctga aagacagctt tgacgattcc agtgctaacc tgttcaaact
     7681 gggcgcggag aacatcttcc tcggtcgcaa agcagcaacc aaagaagaag cgattcgttt
     7741 tgctggcgag cagctggtga aaggcggtta cgttgagccg gaatacgttc aggcgatgct
     7801 ggatcgtgaa aaactgaccc cgacttatct gggtgagtct atcgcggtgc cacacggtac
     7861 ggttgaagcg aaagatcgcg tactgaaaac gggcgtggtg ttctgccagt acccggaagg
     7921 cgtgcgcttc ggtgaagaag aagatgacat tgcccgtctg gtgattggta ttgcagcccg
     7981 taacaacgag cacattcagg ttatcaccag cctgaccaat gcactggatg atgagtccgt
     8041 catcgagcgt ctggcacaca ccaccagcgt ggatgaagtg ctggaactgc tggcaggtcg
     8101 taagtaatcc aatcccaccc tctccacatg gagaaggtgg ggttaattgc ctgatgcgct
     8161 acgcttatca ggcctacagg atgcatcaca atttgttgaa tttgcacgtt cttgtaggcc
     8221 ggataaggcg cttacgccgc atccggcgcg gcccctctcc tcacggagag ggtttgggtg
     8281 agggaaaagc ctcaccccag ccctctcggg taaaaacatt gatgaaggtt aatactatga
     8341 aagcattaca ttttggcgca ggtaatatcg gtcgtggctt tatcggtaaa ctgctggcag
     8401 acgcgggtat ccaactgacg tttgccgatg tcaatcaggt ggtacttgat gccctgaatg
     8461 cccgtcatag ctatcaggtt catgtggttg gtgaaaccga gcaggtagat accgtttccg
     8521 gcgtcaatgc tgtcagcagc attggtgatg atgtcgttga tctgattgct caggtcgatt
     8581 tagtcactac cgccgttggc ccggttgtgc tggaacgtat tgctccggct atcgccaaag
     8641 ggctggtgaa acgcaaagaa caaggtaatg aatccccgct gaacatcatc gcctgtgaaa
     8701 acatggtacg cggtaccacg cagctgaaag gccatgtgat gaacgccctg ccggaagacg
     8761 ccaaagcgtg ggtagaagaa cacgttggct ttgtcgattc cgccgttgac cgcatcgtac
     8821 cgccttcggc ttcggcaact aacgatccgc tggaagtgac ggtagaaact ttcagcgaat
     8881 ggattgtcga taaaacgcag ttcaaaggca cactgccgaa catcccaggc atggagttaa
     8941 ccgacaacct gatggcattt gtcgaacgta aactcttcac cctgaacacg ggtcatgcta
     9001 taaccgcgta cctcggaaaa ctggctggtc atcagaccat tcgtgacgct attctcgacg
     9061 agaaaatccg cgcggtggta aaaggtgcga tggaagaaag tggtgcggta ctgatcaagc
     9121 gctacggctt tgacgcagac aaacatgcgg cgtacatcca gaaaatcctc ggtcgttttg
     9181 agaacccgta tctgaaagat gatgtagagc gcgtaggccg tcagccgctg cgtaaactga
     9241 gtgctggcga ccgtctgatc aagccactgc tcggtacgct ggaatacagc cttccgcaca
     9301 agaatctgat tcaggggatt gctggtgcaa tgcacttccg cagtgaagat gatccgcagg
     9361 ctcaggaact ggcagcactg atcgctgaca aaggtccgca ggcggcgctg gcacagattt
     9421 ccggtcttga tgccaacagc gaggttgtat ccgaggcggt aaccgcttat aaagcaatgc
     9481 aataatggtg gaccaggcgc aggacaccct gcgcccgaat aacagattgt cagatatgca
     9541 ggcaacaatg gaacaaaccc aggcctttga aaaccgtgtg cttgagcgtc tgaatgctgg
     9601 caaaaccgtg cgaagctttc tgatcaccgc cgtcgagctt ttgaccgagg cggtaaatct
     9661 tctggtgctt caggtattcc gcaaagacga ttacgcggtg aagtatgctg tagaaccgtt
     9721 actcgacggc gacggtccgc tgggcgatct ttctgtgcgt ttaaaactca tttacgggtt
     9781 gggcgtcatt aaccgccagg aatacgaaga tgcggagctg ctgatggcat tgcgtgaaga
     9841 actaaatcac gacggcaacg agtacgcctt taccgacgac gagatccttg gaccctttgg
     9901 tgaactgcac tgcgtggcgg cgttaccacc gccgccacag tttgaaccag cagactccag
     9961 tttgtatgca atgcaaattc agcgctatca acaggctgtg cgatcaacaa tggtcctttc
    10021 actgactgag ctgatttcca gaatcagctt aaaaaaagcc tttcaaaagt aagcaacgtc
    10081 tgcttactgc ccctctacct gcttcggccg ataaagccga cgataatact ccagacgttg
    10141 tagatataac ggcacgcttt catcgggtat tccggacgga atcgcgttac ggggaggaag
    10201 ttttttcaga tactcccgga acgcctggct tgatgccatg aaatctacgg ctttatcgat
    10261 aagaagcgga acgttttcac ctaatttgcc catgatgata tctccgtatt accccgcgcc
    10321 gggaatgcgc ggccgccaat tttagtttag gttcccggta aagatgacat taaggaaaac
    10381 gtgctgaatc ctcaataatc atccgctgct gtttattatc ttttttctat agatttctta
    10441 attaatcaac gaattgcgta gtttttgagc atgttaatga ttacgttgca gaactattac
    10501 aatgtcatca gttgtgaaca aagcacctgg tcgcgcatac taggggctat aaatttatct
    10561 ttatcagaag ccatcacatg aaagaagtcg aaaaaaacga aatcaaacgt ctcagcgatc
    10621 gcctggacgc catccgccac cagcaggccg atctatcgct ggttgaagcc gcagacaaat
    10681 atgccgagct ggaaaaagag aaagccacgc tggaagcaga aattgctcgc ctgcgtgagg
    10741 ttcatagcca aaaactgagt aaagaagcac agaagctgat gaagatgccg tttcagcgcg
    10801 caattaccaa aaaagagcag gctgatatgg gcaagctgaa gaaaagtgtt cgcggactgg
    10861 tggttgtgca cccaatgacc gcgctgggcc gtgaaatggg cctgcaggag atgactggat
    10921 tctctaagac tgcgttttaa gcgcctcaga cgactggttc gatcatatca acccgcatct
    10981 ggcttgcctt agctggtcaa tgcggggact ctcctgtcta ctcgcctaat taccaaacaa
    11041 ttcactccct ttacgtcgat ttaccgctgg ttgatattct gaaaatgcat ttttaccgaa
    11101 atagccacct ataaacaata aaaatatata gaagatatca tttattaatt aacaagcatt
    11161 tacgtctctt tttttcggat ttaattgtga aattttaatc atcatattca tacaataaat
    11221 tatctgtaaa ttttttccca gacatggttt ctttcatatt tgacatttat taataatcat
    11281 cattagaatc cacgaaaata atatattcat acaaacacag acatcttttg aaacgaatga
    11341 tacattttta tgatgaatgc ttaaaacaat atttaatcga cacacatcac tgtcattaat
    11401 gtaaagcaac atagatcacc aataatacaa tggacaactc taccggcatt gatcgctggt
    11461 cggaggaaat aaggattttt acatgaactt gaagaagatt tttttcagtg ctgtcactgt
    11521 ttcagttctc tgcgcattaa cgggatgtga ctacattgaa gaaggcaagc cagaaagtag
    11581 tttattgaaa caacaagaag aacataacaa taaaattgat ctacttgaaa aacaacaagc
    11641 acagctaaag agtcaactgg aaactattca aaaacaacaa actggcataa taagcagtac
    11701 aaagacttta actcacgtaa ttaaatccgt taaagatcaa caaaacactt ttatttttac
    11761 cgagtttaac ccagcaaaaa cgaagtattt catcctgaac aatggttcgg tggctttagc
    11821 gggtcgagtg ttatccattg acgccacaga gaatggcagt gttattcata tttcactggt
    11881 caacttatta agtacaccta tctcaaatat tggttttaat gcgacatggg gtggtgaaaa
    11941 acctgtcgat gccaaagagt ttgcccggtg gcaacaatta cttttcaaca catcaatgaa
    12001 atccacattg aaattattac caggtcaatg gcaagacatt aatttgaccc tgaagggtgt
    12061 atcgcccaat aacctgggat atctgaagtt agccatcaac atggaaaata ttcagttcga
    12121 caatcttccc tctgctgaaa atcggcagaa aagaagcaaa aaataaatgc agttagtacg
    12181 cttataacaa ggaatagcga gcatcattaa tgaacaaaat atttaaagtt atctggaacc
    12241 ctgcgacagg gaattatact gttaccagcg aaacggcaaa aagccgtggc aagaaatctg
    12301 ggcgcagtaa gctgttaatt tctgcgctgg ttgcgggtgg aatgttgtcg tcgtttgggg
    12361 cattggcgaa tgccgggaat gacaacggtc agggtgttga ttacggtagt ggatcagctg
    12421 gcgacggctg ggttgctata ggcaaagggg cgaaagcaaa tacttttatg aacaccagtg
    12481 gttccagtac tgctgtgggt tatgacgcta tagctgaagg ccaatatagc tctgccatcg
    12541 ggtcaaaaac ccatgcgatt ggcggtgcat caatggcctt tggggttagt gcaatatcag
    12601 aaggcgatag aagtatagca ctgggtgcct cttcgtattc attgggccaa tactcaatgg
    12661 ccctcggccg ttattcaaaa gcattgggta aattgtctat tgctatgggg gactcttcca
    12721 aagcggaagg agcaaacgcc attgccctgg gaaatgccac taaagctact gagattatga
    12781 gtattgctct tggcgacacc gccaatgcgt caaaagcgta ttcaatggcg ctgggagcaa
    12841 gtagcgtcgc atctgaagaa aacgcaattg ccctggggcg tagcagtgta gctagcggta
    12901 ctgacagcct cgcatttggc agacaatcac ttgccagcgc agcgaacgct attgcgatag
    12961 gtgctgagac cgaagccgct gaaaatgcaa ctgctattgg caataatgcg aaggcaaaag
    13021 ggactaatag catggcaatg gggttcggaa gccttgccga taaagtcaat actatcgcat
    13081 taggaaatgg cagccaggct ctggcagata atgcaatcgc cataggccag ggcaacaaag
    13141 ctgatggcgt ggatgccatc gctctgggta atggtagcca gtcgagaggc ttaaacacca
    13201 ttgccttagg cacagccagt aatgcaactg gtgataagag tcttgcgctt ggtagtaata
    13261 gcagtgccaa cggtattaac tctgtcgcac tgggcgcaga ttccattgcg gatttagaca
    13321 ataccgtctc tgtcggcaat agttcattaa aacgcaagat cgttaatgtg aaaaatggcg
    13381 cgatcaagtc tgacagttac gatgccatta atggttcaca gctttatgcc attagcgact
    13441 cggtagcaaa aaggcttgga ggaggggctg cagtagatgt tgatgacggt actgttacag
    13501 caccaaccta caatttaaaa aatggtagca aaaataacgt aggggctgcg ctcgctgtac
    13561 ttgatgaaaa caccctgcaa tgggaccaaa ccaaaggcaa atacagcgct gctcatggta
    13621 ctagtagccc aactgccagc gtaatcaccg atgttgcgga tggcacgatt tcagcctcca
    13681 gtaaggatgc ggttaacggt tcccaactga aagctaccaa tgacgatgtc gaagccaaca
    13741 ccgccaatat cgctactaat accagcaaca ttgccacgaa tacggcaaat attgccacca
    13801 ataccaccaa tatcaccaac ctgacggatt ccgttggtga ccttcaggct gatgccctgc
    13861 tctggaacga aactaaaaag gcattcagtg cagctcacgg ccaggatacc accagcaaaa
    13921 tcaccaacgt taaagatgcc gacctgacgg ctgacagcac tgatgctgtt aacggctctc
    13981 agctgaaaac caccaacgat gctgtggcga cgaataccac caatatcgcc aataacactt
    14041 ccaatattgc cactaacacc accaacatct ctaacctgac tgagacggtg actaatcttg
    14101 gtgaggatgc gctgaaatgg gataaggaca atggtgtatt cacggcagct catggcaccg
    14161 agaccaccag caaaatcacc aacgttaaag atggcgacct gacgactggc agcaccgatg
    14221 ccgttaacgg ctctcagctg aaaaccacca acgatgccgt ggcgacgaat accaccaata
    14281 tcgccactaa caccaccaac atctctaatc tgactgagac ggtgactaat cttggtgagg
    14341 atgcgctgaa atgggataag gacaatggtg tcttcactgc agctcatggc aacaataccg
    14401 ccagcaaaat caccaatatc ctggacggca cagtcactgc aaccagttcc gatgccatta
    14461 acggtagcca gctttatgac ttaagcagca atatcgccac ctacttcggc ggcaatgctt
    14521 ctgtgaatac tgacggtgtg tttaccggtc caacctacaa aatcggtgaa acaaattatt
    14581 ataacgtcgg cgatgcactg gctgcgatta actcctcatt tagcacgtct ctcggcgatg
    14641 ctctgctttg ggatgccacc gcaggtaaat tcagtgccaa acacggtact aatggtgacg
    14701 caagcgtgat cactgatgtc gcagatggtg aaatttcaga ctccagttct gacgcagtaa
    14761 acggctcaca actccacggc gtgagcagtt atgttgttga tgcgctgggg ggtggtgccg
    14821 aagtcaatgc agacggcacc atcactgcgc cgacgtacac cattgctaat gctgattacg
    14881 ataatgtcgg tgatgccctg aatgctatcg ataccactct tgacgacgct ctgctctggg
    14941 atgcggacgc cggtgaaaat ggtgcattta gcgccgctca cggaaaagat aaaactgcca
    15001 gtgtaatcac taacgtcgct aacggtgcaa tctctgctgc cagcagcgac gcgattaacg
    15061 gctcacaact ctataccacc aataagtaca tcgctgatgc gctgggtggt gacgcagaag
    15121 tcaacgccga cggcaccatc accgcaccga cttacaccat tgcgaacgcc gagtacaaca
    15181 acgtcggtga cgccctggat gcgcttgatg ataacgccct gctgtgggat gagactgcca
    15241 atggcggtgc tggagcctac aatgccagcc atgacggtaa agccagcatc atcactaatg
    15301 tcgctaatgg cagtattagt gaggacagta ccgatgcagt gaacggttct cagttgaatg
    15361 cgacgaatat gatgattgag cagaacaccc aaattatcaa tcagctcgct ggtaacaccg
    15421 acgcaaccta tatccaagaa aacggtgcgg gtattaacta tgtgcgtact aacgacgacg
    15481 gcttagcgtt caacgacgcc agcgcacagg gtgttggcgc tacagctata ggttataact
    15541 ctgtcgccaa aggcgatagc agcgtagcta ttggtcaggg cagctacagc gacgttgata
    15601 cgggtatcgc cctgggtagc agctctgttt ccagccgagt gattgccaaa ggctcccgtg
    15661 acaccagcat aacggaaaat ggcgttgtta ttggttacga caccacggat ggcgaactgc
    15721 tcggtgcatt gtctatcggt gatgacggta aatatcgtca aatcatcaac gtagccgatg
    15781 gttccgaagc ccatgacgcc gttacggttc gtcaattgca gaatgcgatt ggtgctgtag
    15841 caacgacacc gtccaaatac ttccatgcta attcaacgga agaagattca ctggcagtgg
    15901 gaactgactc gctggcaatg ggtgcgaaaa ccatcgtgaa tggcgataaa ggtattggta
    15961 tcggttatgg tgcctacgtg gacgcgaatg cacttaacgg cattgccatt ggtagcaatg
    16021 cgcaagtcat tcatgtcaac agtattgcga taggtaatgg ttctacgacc actcgtggcg
    16081 ctcaaaccaa ttatacagcc tacaacatgg acgcaccgca gaactctgtc ggtgaattct
    16141 cagttggtag tgcggatggt caacgtcaga tcactaacgt cgcagcaggt tcggctgata
    16201 ccgatgcggt caacgtgggt cagttgaaag taacggatgc gcaggtttcc cagaataccc
    16261 agagcattac taacctggat aatcgggtaa cgaatcttga ttcacgcgtc accaatatcg
    16321 aaaacggtat tggcgatatc gtcaccaccg gtagcaccaa gtacttcaag accaataccg
    16381 atggtgtaga tgccagcgcg cagggtaaag atagcgttgc tattggttct ggttccattg
    16441 ctgccgctga caacagcgtc gcactgggta ccggttccgt tgcagaggaa gaaaatacaa
    16501 tctctgtagg ttcttctact aaccaacgcc gtatcaccaa cgtagctgcc ggtgttaatg
    16561 ccaccgatgc ggttaacgtg tcgcagctga aatcttctga agcaggcggc gtgcgctacg
    16621 acaccaaagc tgatggttct atcgactata gcaatatcac cctcggtggc ggcaacggcg
    16681 gtacgactcg tatcagcaac gtctccgctg gcgtcaacaa caacgacgcg gtgaattacg
    16741 cgcagttgaa gcaaagcgtg caggaaacga agcaatacac cgatcagcga atggttgaga
    16801 tggataacaa actgtctaaa actgaaagca agttgagcgg tggtatcgct tctgcaatgg
    16861 caatgaccgg tctgccgcag gcttacactc caggtgccag catggcctct attggtggcg
    16921 gtacttacaa cggtgaatcg gcagttgctt taggtgtatc gatggtgagc gccaatggtc
    16981 gttgggtcta caaattacaa ggtagtacca atagccaggg tgaatactcc gccgcactcg
    17041 gtgccggtat tcagtggtaa tgatccatta agttagtgtg actaagggca aacgccaaat
    17101 tgcctgatgc gttacgcgca tcaggcaaga taaggcgctc cgggtcaaca atctaagggc
    17161 aattctctga tgaggattgc ccttttcttt accagacatc tccccccaca agaattggcc
    17221 ctaccaattc ttcgcttatc tgacctctgg ttcacaattc cccaattaaa actcacatca
    17281 atattgccaa tacataacat ttagttaacc attcattgtc attatcccta cacaacacaa
    17341 ttggcagtgc cacttttaca caacgtgtga caaggagatg agcaacagac tcattacacg
    17401 atgtgcgtgg actccaggag acctgcaatg aatctctggc aacaaaacta cgatcccgcc
    17461 gggaatatct ggctttccag tctgatagca tcgcttccca tcctgttttt cttctttgcg
    17521 ctgattaagc tcaaactgaa aggatacgtc gccgcctcat ggacggtagc aatcgccctt
    17581 gccgtggctt tgctgttcta taaaatgccg gtcgctaacg cgctggcctc ggtggtttat
    17641 ggcttcttct atggtttgtg gcccatcgcg tggatcatta ttgcagcagt gttcgtctat
    17701 aagatctcgg tgaaaaccgg gcagtttgac atcattcgct cgtctattct ttcgataacc
    17761 cctgaccagc gtctgcaaat gctgatcgtc ggtttctgtt tcggcgcttt ccttgaagga
    17821 gccgcaggct ttggcgcacc ggtagcaatt accgccgcat tgctggtcgg cctgggtttt
    17881 aaaccgctgt acgccgccgg actgtgcctg attgttaaca ccgcgccagt ggcatttggc
    17941 gcgatgggca ttccaatcct ggttgccgga caagtaacag gcatcgacag ctttgagatt
    18001 ggccagatgg tggggcggca gctaccgttt atgaccatta tcgtgctgtt ctggatcatg
    18061 gcgattatgg acggctggcg cggtatcaag gagacgtggc ctgcggtcgt ggttgcgggc
    18121 ggctcgtttg ccatcgctca gtaccttagc tctaacttca ttgggccgga gctgccggac
    18181 attatctctt cgctggtatc actgctctgc ctgacgctgt tcctcaaacg ctggcagcca
    18241 gtgcgcgtat tccgttttgg tgatttgggg gcgtcacagg ttgatatgac gctggcccac
    18301 accggttaca ctgcgggtca ggtgttacgt gcctggacac cgttcctgtt cctgacagct
    18361 accgtaacac tgtggagtat cccgccgttt aaagccctgt tcgcatcggg tggtgcgctg
    18421 tatgagtggg tgatcaacat tccggtgccg tacctcgata aactggttgc ccgtatgcca
    18481 ccagtggtca gcgaggctac agcctatgcc gccgtgttta agtttgactg gttctctgcc
    18541 accggcaccg ccattctgtt tgctgcactg ctctcgattg tctggctgaa gatgaaaccg
    18601 tctgacgcta tcagcacctt cggcagcacg ctgaaagaac tggctctacc catctactcc
    18661 atcggcatgg tgctggcgtt cgcctttatc tcgaactatt ccggactgtc atcaacactg
    18721 gcactggcac tggcgcacac cggtcatgca ttcaccttct tctcgccgtt cctcggctgg
    18781 ctgggggtat tcctgaccgg gtcggatacc tcatctaacg ccctgttcgc cgcgctgcaa
    18841 gccaccgcag cacaacaaat tggcgtctct gatctgttgc tggttgccgc caataccacc
    18901 ggtggcgtca ccggtaagat gatctccccg caatctatcg ctatcgcctg tgcggcggta
    18961 ggcctggtgg gcaaagagtc tgatttgttc cgctttactg tcaaacacag cctgatcttc
    19021 acctgtatgg tgggcgtgat caccacgctt caggcttatg tcttaacgtg gatgattcct
    19081 taatgattgt tttacccaga cgcctgtcag acgaggttgc cgatcgtgtg cgggcgctga
    19141 ttgatgaaaa aaacctggaa gcgggcatga agttgcccgc tgagcgccaa ctggcgatgc
    19201 aactcggcgt atcacgtaat tcactgcgcg aggcgctggc aaaactggtg agcgaaggcg
    19261 tgctgctcag tcgacgcggc ggcgggacgt ttattcgctg gcgtcatgac acatggtcgg
    19321 agcaaaacat cgtccagccg ctaaaaacgc tgatggccga tgatccggat tacagtttcg
    19381 atattctgga agcccgctac gccattgaag ccagcaccgc atggcatgcg gcaatgcgcg
    19441 ccacacctgg cgacaaagaa aagattcagc tttgctttga agcaacgcta agtgaagacc
    19501 cggatatcgc ctcacaagcg gacgttcgtt ttcatctggc gattgccgaa gcctcacata
    19561 acatcgtgct gctgcaaacc atgcgcggtt tcttcgatgt cctgcaatcc tcagtgaagc
    19621 atagccgtca gcggatgtat ctggtgccac cggttttttc acaactgacc gaacaacatc
    19681 aggctgtcat tgacgccatt tttgctggtg atgccgacgg ggcgcgtaaa gcaatgatgg
    19741 cgcaccttag ttttgttcac accaccatga aacgattcga tgaagatcag gctcgccacg
    19801 cacggattac ccgcctgccc ggtgagcata atgagcattc gagggagaaa aacgcatgat
    19861 tatttccgca gccagcgatt atcgcgccgc agcgcaacgc attctgccgc cgttcctgtt
    19921 ccactatatg gatgggggtg catattctga atacacgctg cgccgcaacg tggaagattt
    19981 gtcagaagtg gcgctgcgcc agcgtattct gaaaaacatg tccgacttaa gcctggaaac
    20041 gacgctgttt aatgagaaat tgtcgatgcc ggtggcgcta ggtccggtag gtttgtgtgg
    20101 catgtatgcg cgacgcggcg aagttcaggc agccaaagcg gcggatgcgc atggcattcc
    20161 gtttactctc tcgacggttt ccgtttgccc gattgaagaa gtcgcgccag ccatcaagcg
    20221 cccaatgtgg ttccagcttt atgtactgcg cgatcgcggc tttatgcgta acgcgctgga
    20281 gcgagcaaaa gcagcgggtt gttcgacgct ggttttcacc gtggatatgc cgacaccggg
    20341 agcgcgttat cgtgatgcgc attctggaat gagcggcccg aacgcggcaa tgcgccgcta
    20401 tttgcaggcg gtgacgcatc cgcaatgggc gtgggatgtg ggcctgaacg gtcgtccgca
    20461 tgatttagga aatatttcgg cttacctcgg caaaccgacc ggactggaag attacatcgg
    20521 ctggctgggg aataacttcg atccgtccat ctcatggaaa gaccttgagt ggatccgcga
    20581 tttctgggat ggcccgatgg tgatcaaagg gatcctcgat ccggaagatg cgcgcgatgc
    20641 agtacgtttt ggtgctgatg ggattgtggt ttctaaccac ggtggccgcc agctggacgg
    20701 tgtgctctct tctgcccgtg cactgcctgc tattgccgat gcggtgaaag gtgatatcgc
    20761 cattctggcg gatagcggaa ttcgtaacgg gcttgatgtc gtgcgtatga ttgcgctcgg
    20821 tgccgacacc gtactgctgg gtcgtgcttt cctgtatgcg ctggcaacag cgggccaggc
    20881 gggtgtagct aacctgctaa atctgatcga gaaagagatg aaagtggcaa tgacgctgac
    20941 tggcgcgaaa tcgatcagtg aaattacgca agattcgctg gtgcaggggc tgggtaaaga
    21001 gttgcctacg gcactggctc cgatggcgaa agggaatgcg gcatagtcgt ttgagccccc
    21061 tcaccctaac cctctccctc agggagtggg gaccgttcgg cgctgtatgt actccctcac
    21121 tctgaaacga caccgcactc tttttttctc cctcgcccct ccggggtgag gggaaaaggc
    21181 cgcactgctc accaattttc tgctatcctg cccccgcact aagggggcag tatgctaaac
    21241 atcgtacttt acgaaccaga aattccgcca aatactggca acatcatccg tctttgcgct
    21301 aataccggct ttcgtctgca tatcatcgaa ccgatgggat ttgcctggga cgataagcgc
    21361 ctgcgccgcg cggggctgga ctatcacgag tttaccgccg ttacgcgtca tcatgactat
    21421 cgcgcgttcc tcgaagcaga aaatccccag cgcctgtttg ccctcaccac gaaaggtacg
    21481 cctgctcaca gcgccgtaag ctatcaggat ggcgattatc tgatgttcgg cccggaaaca
    21541 cgcggcctgc cagcgagcat tcttgatgcc ctgcccgctg aacaaaaaat tcgcattccg
    21601 atggtgccgg acagccgcag catgaatctg tccaatgcgg tgtcggtagt ggtgtatgaa
    21661 gcctggcggc agctgggata tccgggagcg ttgttgagag attagttact gtatgccgga
    21721 tagatgatta catcgcatcc ggcacgatca caggacatta gatcccatcc ccatactcaa
    21781 atgtatggtt aataccgttg aaatgctggt ccatatccat tgatggctta tcgctgtctg
    21841 gtttaccgac aatacgagcc ggaacgccag cggcggttgt atgcggcggc accggttgca
    21901 gcaccacgga acctgcgcca atcttcgcgc cgcgaccaac ttcaatattg ccgaggattt
    21961 tcgcgcccgc gccaatcatc acaccttcac gaattttcgg gtgacggtca ccaccagatt
    22021 tacccgtacc gccaagcgtc acagattgca gaatcgatac gtcgttttca attaccgccg
    22081 tttcacccac aacgatgccc gtggcgtggt cgagcataat gccacggcca atttttgctg
    22141 ccgggtgaat atcgacctgg aacgtcacag aaacctggtt ttgcagaaag attgccaatg
    22201 cgcgacgccc ctgattccac aaccagtgac cgatgcgata ggcctgcaag gcatgaaaac
    22261 ccttcaggta taacaacggg gttgagtatt tatcgactgc cgggtcgcgg gtacgcaccg
    22321 cctgaatatc acaggccgca gaggcgatca tttccgggtc agcggcgtag gcttcttcca
    22381 ccacttcacg gatagcaata gcaggcataa ttggcgatga cagcttgttc gccagcatgt
    22441 agctcagtgc actaccaagg ttttcgtgct tgagtagcgt cgcgtggtaa aaactggcca
    22501 gcattggctc acagtccgcc agcgttctgg cttcggcttt aatattgttc cagacaattt
    22561 ccagttcttc acacgacatt gcttactcca cacgatgaga taatgaccgg cccgttctgc
    22621 gcgggccggg tcatagcggt aacaaaggtt ccctggggtt agtggctgct gcgctcgtcc
    22681 ttgcgtgcac gacctaataa cgtcaatgct gcctcgcgcg cgttttttcc gcaatataat
    22741 acttgataaa tttcctcggt tattggcatt tcaacgccga agcgatgcgc cagttcgcgg
    22801 acttctttcg tattgcggta gccttccacc acctgaccaa tcttctcctg cgcgctttgt
    22861 acatccatgc cctgaccgag catcatgcca aaacggcggt tacgcgactg gttgtcggta
    22921 caggtaagca ccagatcgcc aagccccgcc atgcccataa aggtggcagg atcggcaccc
    22981 agcgccgcac caagacgcga catttcagcc agcccacggg tgatcagcgc cgtacgcgca
    23041 ttcgcaccaa aaccgatacc gtcggacatc cccgcgccaa tggcaatgac gtttttcacc
    23101 gcaccgccaa gctgcacacc gatgaaatcc gggttgctgt aaacgcggaa acttttgcca
    23161 cagtgcagca attgttggag atcatcggca aaggtctggt cggtagaggc caacgaaata
    23221 gccgtcggta aacctgccgc cagttctttc gcaaacgttg ggccagagat aaccgccagc
    23281 ggaatttgat cgcctaaggc ctcacgcgcc acgtcctgta acagacgtcc ggtttccgct
    23341 tccagccctt tggtcgccca caccagacgc gcatcaggac gcatcagcgg tttaatctgg
    23401 cgcagcactt caccaaagac atggctgggt acgacgacga gaatattacg gctggctgcc
    23461 agcgcagtgg cgagatcgct ttcaagatgg agcgtatcgg gaaaaggcac atcggggaga
    23521 aacgcggcgt tacagcggtc gcgttcaagc gttgcgatat gttcagggtc atggccccag
    23581 aggacaacct cgtggccatt tcttgccagg gtaatggcaa gagcggtgcc gtacgagccg
    23641 gcaccgatca cagtcattga agcattacgt tggttcatca ggcatcctga tgttcttcag
    23701 taccttcgcc agcctgctgc tgcaaatagt tcatgaacag cgcatcgaag ttaaccggcg
    23761 caaggttcag ttgcgggaat gtaccgcggg ataccatgct ggtgatgcac tcacgagcat
    23821 acgggaacag aatgttcggg cagtatgcgc ccaggcaatg tgccatctgg gtgccttcga
    23881 tacccgcgat ggagaaaata ccgccctgct gaacttcaca caggaacgcg gtttcttcgc
    23941 ccagggaggc cgttacggta acacgcagta ccacttcgta aacgtcatct gccagttggg
    24001 aagatgccgt atccagatca agtttaactt ctggctgcca atctttctgg aaaacgtgcg
    24061 gcgcgttcgg cgcttcgaaa gaaatatcct tggtataaat acgttggatc tggaaagtca
    24121 tttcggtgtt gttttgttct gacatgtgta gaaaaccctt aagtgttgtc cttaaatact
    24181 gcgcaatgta atgccaacgt tcacacgtta tttcagcagg ggatccagtc caccacgtgc
    24241 atccaatgca tacaagtcat cacagccgcc aatgtgctgt gcgtcaataa aaatctgggg
    24301 aaccgtggtg cgaccgctgc gtttgatcat ctcttcacgc ttggcggcat tgccatcgat
    24361 cggcaactcc tgaaaactca cgcccttgct gctcagcagt gcttttgcac gatggcaata
    24421 cgggcaggtt tctttggtat agatttcaac attggccata atttagctcc tgttttttta
    24481 ccctgaaatt tcatgttgca gggcggctgc aaaatggagt gtccccagaa gcttataaca
    24541 ataagtcgct gggggcctaa ttgcagctaa cggcctgcat catgaaagac gacaggtaaa
    24601 ttatttgccg cgcaccaaag gcaagttttc gccagcccag ccagcgacgc cttctttcag
    24661 cacgaatact tgcgcaaaac cagctttcgt cagtgcgttt gcaggctcct cgcactgcat
    24721 gccagaaccg tctaccacga taaccggttt gtctttgtgc ttctcaagct caccaacatt
    24781 gttggctttg atttcgctcg gcaacagatt gatagaacct gcgatatggc ctttacggaa
    24841 gtcatcacgc tgacgtaaat ccacaaccac agcgtcttct ttgttgatca gacgcgtagc
    24901 ttcaccacga gtaatcacct tcactttcga ggtcaggctc ttaaacgtag tcacaagaac
    24961 cgccaccagt aacgcgatcc aggcgatact cagtatggga tgacggccaa caaattgcat
    25021 aatttcttgc atggggggta acaactcccg acgtagtgat taaaaaaacc aggaaaggag
    25081 tatacctgcg cggtgcggca aatacagcca gcgcgttaac tggaatgcaa ttttgcgggg
    25141 cacgacgaaa aaaaagcgca aatctggcat cggcttgccg cgagcggtcg tatattttga
    25201 tcttcagagg ctattttatc gattcagctg tagtaaaatt acgcaaattt tgactcttga
    25261 gtatgaggtt gtcgcaatgt cggtttctaa aaaacctatg gtactggtga ttctggatgg
    25321 ctatggctat cgtgaagaac agcaggataa cgccattttt agtgctaaaa ccccggtaat
    25381 ggatgcactg tgggccaatc gtccgcatac cctgatcgac gcttccggtc tggaagtcgg
    25441 tctgcctgac cgtcagatgg gtaactccga agtaggccac gttaacctgg gtgccggccg
    25501 catcgtgtat caggacctga ctcgtctgga cgttgaaatc aaagatcgcg ctttctttgc
    25561 taatccagtg ctgactggcg cggtagataa agcgaaaaac gcaggtaaag cagtacacat
    25621 tatgggtctg ctctccgcag gcggcgtaca cagccacgaa gatcacatca tggcgatggt
    25681 agaactggca gctgaacgcg gcgcagaaaa aatctacctg cacgcattcc ttgacggtcg
    25741 cgacaccccg ccgcgcagtg ctgaatcctc gctgaaaaaa tttgaagaga aatttgctgc
    25801 gctgggtaaa ggccgcgtag cgtccatcat tggtcgttac tacgcgatgg accgcgataa
    25861 ccgttgggat cgcgttgaaa aagcctatga cctgctgact ctggcacagg gtgagttcca
    25921 ggccgatact gccgttgctg gtttgcaggc agcttatgct cgcgacgaaa acgacgagtt
    25981 cgtgaaagcg accgttattc gtgcagaagg tcagccagat gcggcaatgg aagacggcga
    26041 tgcgctgatt ttcatgaact tccgtgctga ccgcgcgcgc gaaatcactc gtgcattcgt
    26101 aaacgcagat ttcgacggct tcgcgcgtaa gaaagtggtt aacgtcgatt tcgtgatgct
    26161 gaccgaatac gccgctgaca tcaaaactgc ggttgcttac ccacccgctt ccctggttaa
    26221 caccttcggc gagtggatgg cgaaaaacga caaaactcag ttgcgtattt ccgaaaccga
    26281 aaaatatgcc cacgttactt tcttcttcaa cggtggcgta gaagagtcgt tcaaaggcga
    26341 agatcgcatt ctgatcaact caccgaaagt ggctacctac gatctgcaac cggaaatgag
    26401 ctccgcagag ctgaccgaaa aactggttgc ggccatcaag agcggcaaat acgacaccat
    26461 tatctgtaac tatccgaacg gcgacatggt aggtcacacc ggggtgatgg aagcggcggt
    26521 taaagcggtt gaagcactgg atcactgcgt ggaagaagtc gcgaaagcgg ttgagtccgt
    26581 gggtggacaa ctgctgatca ccgctgacca cggtaacgct gaacagatgc gcgatccggc
    26641 aacgggtcag gcacacacgg cacacaccaa cctgccagtt ccgctgattt acgttggtga
    26701 taagaacgtg aaagcggttg caggcggcaa actttctgac atcgcgccga ccatgttgtc
    26761 gctgatgggt atggaaatcc cgcaagagat gactggtaag ccgctgttca tcgtggaata
    26821 atccctcccc atgaggggaa aggcgattaa taccatgaca cgggccgtga aaccgcgcag
    26881 gtttgcaatc aggcccatca tctacgccag cgttcttagc gctggcgtat tgttgtgcgc
    26941 cttttccgcc cacgcggatg agcgtgacca actcaaatct attcaggccg atatcgccgc
    27001 gaaagagcgc gcggtacgcc aaaagcaaca acaacgcgca agcctgctcg cacaattgaa
    27061 aaagcaggaa gaagcgatct ctgaagccac ccgtaagctg cgcgaaacgc aaaacacgct
    27121 caatcaactc aataaacaga ttgatgagat gaacgcgtcg attgccaaac tggagcagca
    27181 aaaagccgcc caggagcgca gcctcgccgc acaactggat gccgcattcc gtcagggcga
    27241 gcataccggt attcagctaa ttctcagcgg tgaagaaagc cagcgtggac agcgtttaca
    27301 ggcttatttc ggctatctca accaggcgcg acaagaaacc attgcccagt tgaagcaaac
    27361 gcgtgaagaa gtcgccatgc agcgtgctga actggaagag aaacagagcg agcaacaaac
    27421 gctgttatat gaacagcgcg cccaacaggc gaaactgact caggctctga acgagcgtaa
    27481 aaagacgctg gcggggctgg aatcttccat ccagcaaggt cagcagcagc taagtgaact
    27541 gcgtgccaac gaatcccgtc tgcgtaacag cattgcccgt gcggaagccg cggcgaaagc
    27601 gcgtgctgaa cgagaagcgc gtgaggccca ggcggttcgc gaccgccaga aagaagcgac
    27661 gcgcaaaggc accacctata aaccgaccga aagcgaaaaa tcgctgatgt cccgaactgg
    27721 tggcctgggt gcgccgcgtg gtcaggcatt ctggccggtt cgcgggccga cgctgcatcg
    27781 ctatggtgaa cagctacagg gcgaactacg ctggaaagga atggttatcg gtgcctctga
    27841 aggtactgaa gttaaagcga ttgccgacgg tcgggtgatt ctggctgact ggctgcaagg
    27901 ttacggtctg gtggtggtgg ttgagcacgg taaaggcgac atgagtcttt acggctataa
    27961 tcagagcgca ctggtgagcg ttggttcgca ggttcgcgcg ggccagccaa ttgcactggt
    28021 gggcagcagt ggcggtcagg gtcggccttc actctatttc gaaattcgcc gccagggtca
    28081 ggcggtcaat ccacagccgt ggttgggaag ataagttttg tttccatttc gtcgtaacgt
    28141 tcttgcattt gccgctctgt tggcgctctc ctcccccgta cttgctggca aacttgccat
    28201 cgtcattgat gattttgggt atcgcccgca caacgaaaac caggtgctgg cgatgccctc
    28261 cgctatctcc gtcgctgtat tacccgattc accgcacgcc agagagatgg cgaccaaagc
    28321 gcataacagc gggcacgaag tgttgattca tctcccgatg gcaccactga gtaaacagcc
    28381 gctggagaaa aatacgctgc gcccggagat gagcagcgac gaaattgagc gcattattcg
    28441 tagtgcggtc aataacgtgc cctatgccgt ggggatcaac aaccacatgg gtagcaagat
    28501 gacctctaac ctgtttggta tgcagaaagt gatgcaggca ctggagcgtt acaatcttta
    28561 cttcctcgac agcgtaacca tcggtaatac ccaggcgatg cgcgccgcgc aaggtactgg
    28621 cgtgaaggtg atcaaacgga aggtgttcct cgacgattcg cagaacgaag cggacatccg
    28681 tgtgcaattt aatcgcgcaa ttgatctggc gcgtcgcaac ggttcaacca ttgccattgg
    28741 gcatcctcac ccttcaacgg tacgcgtgct acaacagatg gtttataacc tgccgccaga
    28801 cattacgctg gtgaaagcca gcagcttgct gaatgaaccg caggttgata cttctacacc
    28861 gccgaaaaac gctgtgcctg atgcaccgcg taatccgttc cgtggcgtga agctgtgcaa
    28921 accgaagaaa ccgatagaac ctgtttatgc taatcgcttc tttgaagtat taagcgaaag
    28981 catcagccag agcacgctga tcgtttactt ccagcatcag tggcaaggct ggggcaaaca
    29041 gcccgaagcg gcgaagttta acgctagcgc aaattaaagg cgctacgcgt aatgcgatgg
    29101 tgcgaccgcg ttttgtcgcg ccattgccat aagcggaaag accacagtaa cgcctggtaa
    29161 ccgaccttca cgctgcgcac attggtaatc aggcgcttat acataccgga agtaaatatc
    29221 tcggcaatca tccgttggcg ggtaagaata tccggctctt tgcgcaccgc atggcaaaca
    29281 cgcaatgctt cgtaagttat ttgctgatga aattccggat aaatcgtaat tttgtcggca
    29341 taatttcgat ttaatttctc cagcaggcgg gtaatcttaa tatagtgacg ttgataatta
    29401 aggtttttat tcccttgtct atgcaaccga ctcactgacg tattatgcag ataatattta
    29461 tataatgatt gctcggtata tcgcgcacgc agcgcgttaa acatgaattc tgttgtccag
    29521 acaatatcct gatgatgtaa tccggcaata aatttaatgt tatttttaac aataacatca
    29581 cggcgataaa cccccatcca gacaacgtga gtccaacggc gcgaagaaag ccccatccgc
    29641 agccagtccg ggccggttaa tacgccggtt gagcgaaggc gatcgctggg gatggattgc
    29701 caggtttctc ccgtttcacg aaaacaccag tcagcgttgc actgcgccac gtcgaggtca
    29761 tcctctaacg ccatggtcat cagcgtttcg tacatggtgg gatagacctc atcgtcagca
    29821 tcgacaaaag cgacatattt gcccgtcgcc acctcgatcc cacgattacg cgctaccgat
    29881 gcgccagcat tcgcctgatg caacaaacga acgtgcggat agttttctgc gtaatgcttt
    29941 gctatttcaa cagaattatc cgttgaacca tcgttaataa taatgatttc cagagcagtc
    30001 caggtttgcg taattaaaga ttccatacaa gtgcggaaat catcgcccgc attatataac
    30061 ggaataataa cactaagttt attggtgctg ttcatcataa attaccgacg acctctgaag
    30121 ttatcctgac gttttacagg ctttgtatta agaaactatt aagcctgtga gatattattt
    30181 ttacaggtga gagagtcact tcagggtaga gagataatga gagcagctat ataagagatc
    30241 agcgcaatta cgatatcttt attacactga caggatttta catatttaac ggattatcct
    30301 caggcaaaaa ggctggaata ccagcccttg ttcgtgttaa tcccaac
//
LOCUS       DAMZET010000038        29758 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.038, whole genome shotgun sequence.
ACCESSION   DAMZET010000038 DAMZET010000000
VERSION     DAMZET010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.038"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..161)
                     /gene="xylF"
                     /locus_tag="POB34_001805"
     CDS             complement(<1..161)
                     /gene="xylF"
                     /locus_tag="POB34_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="periplasmic substrate-binding component of the
                     ATP-dependent xylose transport system; high affinity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter subunit XylF"
                     /protein_id="HDI5918772.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESL"
     gene            527..1849
                     /gene="xylA"
                     /locus_tag="POB34_001806"
     CDS             527..1849
                     /gene="xylA"
                     /locus_tag="POB34_001806"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDI5918773.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            1921..3375
                     /gene="xylB"
                     /locus_tag="POB34_001807"
     CDS             1921..3375
                     /gene="xylB"
                     /locus_tag="POB34_001807"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDI5918774.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGVQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECALLEERVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCYALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            3544..3885
                     /locus_tag="POB34_001808"
     CDS             3544..3885
                     /locus_tag="POB34_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918775.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            3931..4368
                     /locus_tag="POB34_001809"
     CDS             3931..4368
                     /locus_tag="POB34_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HDI5918776.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(4410..5405)
                     /gene="wecH"
                     /locus_tag="POB34_001810"
     CDS             complement(4410..5405)
                     /gene="wecH"
                     /locus_tag="POB34_001810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HDI5918777.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            5580..5879
                     /locus_tag="POB34_001811"
     CDS             5580..5879
                     /locus_tag="POB34_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HDI5918778.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            5974..6885
                     /gene="glyQ"
                     /locus_tag="POB34_001812"
     CDS             5974..6885
                     /gene="glyQ"
                     /locus_tag="POB34_001812"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDI5918779.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            6895..8964
                     /gene="glyS"
                     /locus_tag="POB34_001813"
     CDS             6895..8964
                     /gene="glyS"
                     /locus_tag="POB34_001813"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDI5918780.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            9290..9442
                     /gene="hokA"
                     /locus_tag="POB34_001814"
     CDS             9290..9442
                     /gene="hokA"
                     /locus_tag="POB34_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HDI5918781.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(9630..9842)
                     /gene="cspA"
                     /locus_tag="POB34_001815"
     CDS             complement(9630..9842)
                     /gene="cspA"
                     /locus_tag="POB34_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HDI5918782.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(10123..10413)
                     /locus_tag="POB34_001816"
     CDS             complement(10123..10413)
                     /locus_tag="POB34_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HDI5918783.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            10847..11557
                     /locus_tag="POB34_001817"
     CDS             10847..11557
                     /locus_tag="POB34_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HDI5918784.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(11607..12581)
                     /gene="ghrB"
                     /locus_tag="POB34_001818"
     CDS             complement(11607..12581)
                     /gene="ghrB"
                     /locus_tag="POB34_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HDI5918785.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(12685..13344)
                     /locus_tag="POB34_001819"
     CDS             complement(12685..13344)
                     /locus_tag="POB34_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HDI5918786.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            13497..15830
                     /gene="bisC"
                     /locus_tag="POB34_001820"
     CDS             13497..15830
                     /gene="bisC"
                     /locus_tag="POB34_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HDI5918787.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGKTVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYIGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(15799..16239)
                     /locus_tag="POB34_001821"
     CDS             complement(15799..16239)
                     /locus_tag="POB34_001821"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI5918788.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(16236..16799)
                     /gene="tag"
                     /locus_tag="POB34_001822"
     CDS             complement(16236..16799)
                     /gene="tag"
                     /locus_tag="POB34_001822"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDI5918789.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            16957..17655
                     /locus_tag="POB34_001823"
     CDS             16957..17655
                     /locus_tag="POB34_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDI5918790.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            17884..19092
                     /locus_tag="POB34_001824"
     CDS             17884..19092
                     /locus_tag="POB34_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5918791.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            19416..21107
                     /gene="eptB"
                     /locus_tag="POB34_001825"
     CDS             19416..21107
                     /gene="eptB"
                     /locus_tag="POB34_001825"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HDI5918792.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            21199..21275
                     /locus_tag="POB34_001826"
     tRNA            21199..21275
                     /locus_tag="POB34_001826"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21233..21235,aa:Pro,seq:cgg)
     gene            21310..21443
                     /locus_tag="POB34_001827"
     ncRNA           21310..21443
                     /ncRNA_class="other"
                     /locus_tag="POB34_001827"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            22189..23796
                     /gene="dppA"
                     /locus_tag="POB34_001828"
     CDS             22189..23796
                     /gene="dppA"
                     /locus_tag="POB34_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HDI5918793.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            24104..25123
                     /gene="dppB"
                     /locus_tag="POB34_001829"
     CDS             24104..25123
                     /gene="dppB"
                     /locus_tag="POB34_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HDI5918794.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            25133..26035
                     /gene="dppC"
                     /locus_tag="POB34_001830"
     CDS             25133..26035
                     /gene="dppC"
                     /locus_tag="POB34_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HDI5918795.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            26046..27029
                     /gene="dppD"
                     /locus_tag="POB34_001831"
     CDS             26046..27029
                     /gene="dppD"
                     /locus_tag="POB34_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDI5918796.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            27026..28030
                     /gene="dppF"
                     /locus_tag="POB34_001832"
     CDS             27026..28030
                     /gene="dppF"
                     /locus_tag="POB34_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HDI5918797.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSFSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQC
                     "
     gene            complement(28060..29331)
                     /locus_tag="POB34_001833"
     CDS             complement(28060..29331)
                     /locus_tag="POB34_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HDI5918798.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         7229 a         7272 c         7832 g         7425 t
ORIGIN      
        1 agagattctg cctttttcac aaaaatatct cgatcttttt gccagcgttc aagacggaga
       61 tcatcaatcg ccatacctat tttgacttct ttggcgtgtg ccgcaacgtt ggtaagcaga
      121 agtgaggtgc aaagggtgag tagaatgttc tttattttca tggtgtaggg ccttctgtag
      181 ttagaggaca gctttaataa gtaacaatca ccgcgataaa cgtaaccaat ttttagcaac
      241 taaacagggg aaaacaatta cagattttta tctttcgatt acgatttttg gtttatttct
      301 tgatttatga ccgagatctt acttttgttg cgcaattgta cttattgcat ttttatcttc
      361 gaggaattac ccagtttcat catcccattt tattttgcga gcgagcgcac acttgtgaat
      421 tatctcaata gcagtgtgaa ataacataat tgagcaactg aaagggagtg cccaatatta
      481 cgacatcatc catcacccgc ggcattacct gattatggag ttcaatatgc aagcctattt
      541 tgaccagctc gatcgcgttc gttatgaagg ctcaaaatcc tcaaacccgt tagcattccg
      601 tcactacaat cccgacgaac tggtgttggg taagcgtatg gaagagcact tgcgttttgc
      661 cgcctgctac tggcacacct tctgctggaa cggggcggac atgtttggtg tgggggcgtt
      721 taatcgtccg tggcagcagc ctggtgaggc actggcgttg gcgaagcgta aagcagatgt
      781 cgcatttgag tttttccaca agttacatgt gccattttat tgcttccacg atgtggatgt
      841 ttcccctgag ggcgcgtcgt taaaagagta catcaataat tttgcgcaaa tggttgatgt
      901 cctggcagcc aagcaagaag agagcggcgt gaagctgctg tggggaaccg ctaactgctt
      961 tacaaaccct cgctatggcg cgggtgcggc gacgaaccca gatcctgaag tcttcagctg
     1021 ggcggcaacg caagttgtta cagcgatgga agcaacccat aaattgggcg gtgaaaacta
     1081 tgtcctgtgg ggtggtcgtg aaggttacga aacgctgtta aataccgact tgcgtcagga
     1141 gcgtgaacaa ctgggccgct ttatgcagat ggtggttgag cataaacata aaatcggctt
     1201 ccagggcacg ttgcttatcg aaccgaaacc gcaagaaccg actaaacatc aatatgatta
     1261 cgatgccgcg acggtctatg gcttcctgaa acagtttggt ctggaaaaag agattaaact
     1321 gaacattgaa gctaaccacg cgacgctggc aggtcactct ttccatcatg aaatagccac
     1381 cgccattgcg cttggcctgt tcggttctgt cgacgccaac cgtggcgatg cgcaactggg
     1441 ctgggacacc gaccagttcc cgaacagtgt ggaagagaat gcgctggtga tgtatgaaat
     1501 tctcaaagca ggcggtttca ccaccggtgg tttgaacttc gatgccaaag tacgtcgtca
     1561 aagtactgat aaatatgatc tgttttacgg tcatatcggc gcgatggata cgatggcact
     1621 ggcgctgaaa attgcagcgc gcatgattga agatggcgag ctggataaac gcatcgcgca
     1681 gcgttattcc ggctggaata gcgaattggg ccagcaaatc ctgaaaggcc aaatgtcact
     1741 ggcagattta gccaaatatg ctcaggaaca taatttgtct ccggtgcatc agagtggtcg
     1801 ccaggagcaa ctggaaaatc tggtaaatca ttatctgttc gacaaataac ggctaactgt
     1861 gcagtccgtt ggcccggtta tcggtagcga taccgggcat ttttttaagg aacgatcgat
     1921 atgtatatcg ggatagatct tggcacctcg ggcgtaaaag ttattttgct caacgagcag
     1981 ggtgaggtgg ttgcttcgca aacggaaaag ctgaccgttt cgcgcccgca tccactctgg
     2041 tcggaacaag acccggaaca gtggtggcag gcaactgatc gcgcaatgaa agctctgggc
     2101 gttcagcatt ctttgcaaga cgttaaagca ttgggtattg ccggccagat gcacggtgca
     2161 accttactgg atgctcaaca acgggtattg cgccctgcca ttttgtggaa cgacgggcgc
     2221 tgtgcgcaag agtgcgcttt gctggaagag cgagttccgc aatcgcgggt gattaccggc
     2281 aacctgatga tgcccggatt tactgcgcct aaattgctat gggttcagcg gcatgagccg
     2341 gagatattcc gtcaaatcga caaagtatta ttaccgaaag attacttgcg tctgcgtatg
     2401 acgggggagt ttgccagcga tatgtctgac gcagctggca ccatgtggct ggatgtcgca
     2461 aagcgtgact ggagtgacgt catgctgcag gcttgcgact tatctcgtga ccagatgccc
     2521 gcattatacg aaggcagcga aattactggt gctttgttac ctgaagttgc gaaagcgtgg
     2581 ggtatggcga cggtgccagt tgtcgcaggc ggtggcgaca atgcagctgg tgcagttggt
     2641 gtgggaatgg ttgatgctaa tcaggcaatg ttatcgctgg ggacgtcggg ggtctatttt
     2701 gctgtcagcg aagggttctt aagcaagcca gaaagcgctg tacacagctt ttgctatgcg
     2761 ctaccgcagc gttggcattt aatgtctgta atgctgagtg cagcatcgtg tctggattgg
     2821 gtcgcgaaat taaccggcct gtgcaatgtc ccagctttaa tcgctgcagc tcaacaggct
     2881 gatgaaagtg ccgagccagt ttggtttctg ccttatcttt ccggcgagcg tacaccacac
     2941 aataatcccc aggcgaaggg ggttttcttt ggtttgactc atcaacatgg ccccaatgaa
     3001 ctggcgcgag cagtgctgga aggcgtgggt tatgcgctgg cagatggcat ggatgtcgtg
     3061 catgcctgcg gcattaaacc gcaaagtgtt acgttgattg gtggcggggc gcgtagtgag
     3121 tactggcgtc agatgctggc ggatatcagc ggtcagcagc tcgattaccg tacggggggg
     3181 gatgtggggc cagcactggg cgcagcaagg ctggcgcaga ttgcggcgaa tccagagaaa
     3241 tcgctcattg agttgttgcc gcagctaccg ttagaacagt cgcatctacc agatgcgcag
     3301 cgttatgccg cttatcagcc acgacgtgaa acgttccgtc gcctctatca gcaacttctg
     3361 ccattaatgg cgtaaacgtt atcccctgcc tgaccgggtg ggggataatt cacatctata
     3421 tatctcacta attaattaat atttagtatg aatttattct gaaaatcatt tgttaatggc
     3481 atttttcagt tttgtctttc gttggttact cgtaatgtat cgctggtaga tatggagatc
     3541 gttatgaaaa cctcaaaaac tgtggcaaaa ctattatttg ttgtcggggc gctggtttat
     3601 ctggttgggc tatggatctc atgcccattg ctaagtggaa aaggctattt tcttggcgtg
     3661 ttaatgacag caacttttgg taactatgca tatcttcgcg cagaaaaact cgggcaactg
     3721 gataattttt ttacccatat ctgccagtta gttgcgttaa tcactatcgg tctcttgttt
     3781 atcggtgttt taaatgcacc tatcaatgct tatgaaatgg tgatctaccc catcgccttt
     3841 tttgtctgct tgtttggtca aatgcgtttg tttcgctcgg tatgagcaac ataaagctct
     3901 tacacattca ggaatgaaag gaatactgtg atggacaaca aaatatcaac ctattcaccg
     3961 gcctttagta ttgtgtcatg gatagctctc gttggtggta tcgttaccta tctgttaggg
     4021 ctatggaatg cagagatgca gttaaatgaa aaagggtatt attttgccgt actggtgtta
     4081 ggactgtttt ctgctgcgtc ttatcaaaag accgttcggg acaagtatga aggcataccg
     4141 accacttcca tttattatat gacctgcctg actgtcttta ttatctctgt tgcattactg
     4201 atggtaggtc tgtggaatgc gacattatta ctcagtgaaa aaggttttta tggactggct
     4261 ttcttcttaa gcttgtttgg tgcagtagcg gtgcagaaga atattcgtga tgccggaata
     4321 aacccgccaa aagaaacaca ggttacccag gaagaataca gcgaataatt cacgtaagcc
     4381 cggtcagtcc aatgtgaacg ggcttttact taactcacta atctgtttct gtcgattcgt
     4441 tgtaccagca tagaaagtaa caaactcgct gccaacgtcg cgcaaaagat ccaaataata
     4501 tccagtattg gccaattttt aagctcaatt ccccgggtgc gcagcgcatg gataatcaag
     4561 gcgtggaatc cgtatatacc caatgaatgg cgggagatta agccaagtcc gcgaatggta
     4621 cgcgtatcca gcgtgttttt aaccagagtc aatagcgcga ttgcgcagat aaaaaccatc
     4681 ggcccacagt aaagatacca ggtatcggca aaatttccgc gccactgcaa ttcatataat
     4741 gtcccgcgag agataataaa aacccccgtc gcaaacagcg cggcgctcac ccacgacagt
     4801 gctttatgct gtgtgtccat catccctata gcgcggccca acatgccata cagaatgtag
     4861 taaaaagtat cgccattgat atataagtta attggcagcc attcaaaacc gtcaattttc
     4921 tgcggcactg tgtttgggtt agcgataatg ccaatcacca ccattagcac cagcaacatt
     4981 tttccgccga cgttcttcac ctgaatcagc ggtgaaacca gataaatcac cgcaatcgcg
     5041 aagaaaaacc acaagtggta aaacactggc ttttgcagca ggtttttcag cgctaactcc
     5101 atattgatgg aggtaaacag cgcaatgtag agcagtgcga ttgcgctata aaaaatcagg
     5161 cataagccga tacgcaagaa atggcgcggc tgggcgctgc gttcgccaaa aaagagatag
     5221 ccggatatca tgaaaaatag cggcacgctg acacgagagg cagaattcag aacattggcg
     5281 atatcccagg tgacggggct aacactatga gcattggtca cataccaggt agtggtgtga
     5341 atcatcacca ccattaaaca cgctatccct cgcaggttat caatccagta aattttgggc
     5401 tgcatctgtg tctctgtatc tggttaaaaa aagtctgacc gataaatcat tcagttggcg
     5461 cactggaata atctgagttt tatcactaca gcttatagag gcttaaggaa attcgtaaga
     5521 tatcagccac tataccgata taaataataa gactcacctg caaaccagac ggtaatttaa
     5581 tgatgatgaa cgctttcttt ccggcaatgg cgcttatggt gctagtgggt tgttctacac
     5641 cgtcacccgt gcagaaagca caacgggtaa aggttgatcc tctgcgttcg ttgaatatgg
     5701 aagcgttatg caaggatcag gcggcaaaac gttataacac cggcgagcaa aaaatcgacg
     5761 tcaccgcctt cgaacagttc cagggaagct atgaaatgcg cggttatacc ttccgtaaag
     5821 agcagtttgt ctgttctttt gacgcagatg gccatttttt gcatctttcc atgcgttaag
     5881 ccctgctttt tcccgtttcg tactgtatat cttccatcca gcgggtatac tgatcccttc
     5941 ctttaaatcc acacgtatcc agcacgaaat aatatgcaaa agtttgatac caggaccttc
     6001 cagggcttga tcctgacctt acaggattac tgggctcgcc agggctgcac cattgttcaa
     6061 ccattggaca tggaagtcgg cgcgggaacc tctcacccaa tgacctgtct gcgcgcgctg
     6121 gggccagaac cgatggcggc tgcttatgtt cagccttctc gtcgcccgac cgatggtcgc
     6181 tacggcgaaa accccaaccg tttacagcac tactatcagt tccaggtggt cattaagcca
     6241 tcgccggaca atattcagga gctgtacctc ggttctctga aagagctggg catggacccg
     6301 actattcacg acatccgttt cgtggaagat aactgggaaa acccgactct gggtgcctgg
     6361 ggactgggct gggaagtgtg gctaaacggc atggaagtga cgcagttcac ttacttccag
     6421 caggttggtg gtctggagtg taaaccggtt accggcgaga tcacctacgg tctggaacgt
     6481 ctggccatgt acattcaggg cgtagacagc gtttacgacc tggtctggag cgacggcccg
     6541 ctgggtaaaa ccacctacgg cgacgtgttc catcagaacg aagtggagca gtccacttac
     6601 aacttcgaat acgcggatgt ggacttcctg ttcacctgct ttgagcagta cgagaaagaa
     6661 gcgcagcagc tgctggcgct ggaaaatccg ctgccgctgc cagcctacga gcgtattctg
     6721 aaagccgccc acagcttcaa cctgctggat gcgcgtaaag ccatctccgt caccgagcgt
     6781 cagcgctaca ttctgcgcat tcgcaccctg accaaagcag tggcagaagc atactacgct
     6841 tcccgtgaag ccctcggctt cccgatgtgc aacaaagata agtaagaggc ggctatgtct
     6901 gagaaaactt ttctggtgga aatcggcact gaagagctgc caccaaaagc actgcgcagc
     6961 ctggctgagt cctttgctgc gaattttact gcggagctgg ataacgctgg cctcgcacac
     7021 ggcaccgttc aatggtttgc tgctccgcgt cgtctggcgc tgaaagtagc taacctggcg
     7081 gaagcgcaac cggatcgtga aatcgaaaaa cgcggcccgg cgattgccca ggcgttcgac
     7141 gctgaaggca aaccgagcaa agcggcagaa ggttgggcgc gtggttgcgg tattaccgtt
     7201 gaccaggctg agcgtctgac taccgataaa ggcgaatggc tgctgtatcg cgcccatgtg
     7261 aagggcgaaa gcaccgaagc actgctgccg aatatggttg cgacttctct ggcgaaactg
     7321 ccgatcccga aactgatgcg ttggggcgca agcgacgtgc acttcgtgcg tccggtgcac
     7381 accgtgaccc tgctgctggg cgacaaagtc attccggcaa ccattctggg cattcagtcc
     7441 gatcgcgtga ttcgcggcca ccgctttatg ggcgaaccgg aattcaccat cgacaacgcc
     7501 gatcagtatc cggaaattct gcgtgagcgc gggaaagtca tcgccgatta cgaagaacgt
     7561 aaagcgaaga ttaaagccga tgccgaagaa gcagcgcgta agattggcgg taacgctgac
     7621 ttaagcgaaa gcctgctgga agaagtggct tcgctggtgg agtggccggt cgttctgacc
     7681 gcaaaattcg aagagaaatt cctcgcggtg ccggctgaag cgctggttta caccatgaaa
     7741 ggtgaccaga aatacttccc ggtgtatgcg aacgacggca aactgctgcc gaactttatc
     7801 ttcgttgcca acatcgaatc gaaagatccg cagcagatta tctccggtaa cgagaaagtc
     7861 gttcgtccgc gtctggcgga tgccgagttc ttcttcaaca ccgaccgtaa aaaacgtctg
     7921 gaagataacc tgccgcgcct gcaaaccgtg ctgttccagc aacagctggg tacgctgcgt
     7981 gacaaaactg accgcatcca ggcgctggct ggctggattg ctgaacagat tggcgctgac
     8041 gttaaccacg caacccgtgc gggcctgctg tctaagtgtg acctgatgac caacatggtc
     8101 ttcgagttca ccgacaccca gggcgttatg gggatgcact atgcgcgtca cgatggcgaa
     8161 gcggaagatg tcgcggtggc gctgaatgag cagtatcagc cgcgctttgc cggtgatgac
     8221 ctgccgtcta acccggtagc ctgtgcgctg gcgattgctg acaagatgga tactctggcg
     8281 ggtatcttcg gtatcggcca gcatccgaaa ggcgacaaag acccgtttgc gctgcgtcgt
     8341 gccgcacttg gcgtgctgcg aattatcgtt gagaagaacc tcaaccttga tctgcaaacg
     8401 ctgaccgaag aagcagtgcg tctgtatggc gataagctga ctaacgccaa cgtggttgat
     8461 gatgttatcg actttatgct cggtcgcttc cgcgcctggt atcaggacga aggttacacc
     8521 gttgacacca tccaggcggt actggcgcgt cgtccgactc gtccggctga tttcgatgcc
     8581 cgcatgaaag cggtatcgca cttccgtacc cttgaagcag ctgctgcact ggcggcggcg
     8641 aacaagcgtg tctccaacat tttggcgaaa tctgacgaag tgctgagcga ccgcgtgaat
     8701 gcctctaccc tgaaagagcc ggaagaaatt aaactggcga tgcaggttgt ggtgctacgt
     8761 gacaagctgg agccgtactt tgcggaaggt cgttaccagg atgcgctggt cgaactggca
     8821 gagctgcgtg agccggttga tgctttcttc gataaagtga tggtcatggt tgatgacaaa
     8881 gaattgcgtc tcaaccgtct gaccatgctg gagaaactgc gcgaactgtt cctgcgcgtt
     8941 gcggatattt cgctgttgca gtaataacgc cgttattaaa aagcctgcca tctggcaggc
     9001 tttttttatt atcgctaaat aatacagcaa cctttaataa tcttctgctg aataaagatt
     9061 atctcatata ttaattttat gaggtttttt taggattata tcaaggagaa gaaacaaact
     9121 tattaagcta gaatagccac gggtgcttga gactgtttgt ctcaggtatt caccgaaagg
     9181 cagacagaga aaagccccac ctgactataa atcaaagtga ggctacccta tgcctgaaca
     9241 ccataaggtt agcctcttac tcgttggaaa tcaacacagg gggctgggaa tgccgcagaa
     9301 atatagatta ctttctttaa tagtgatttg tttcacgctt ttatttttca cctggatgat
     9361 aagagattca ctgtgtgaat tgcatattaa acaggggagt tatgagctgg cggcattttt
     9421 agcctgcaat ttaaaagagt aagagtcttc ggcgggaagt tattcccgcc tttcttacgg
     9481 cgttgcgcat tctcattgca cccaaattta tttttcacaa aaataatact agattttatt
     9541 acgcgatcga ttatttattt cctgaaaaac aaataaaaaa atccccgcca aatggcaggg
     9601 atcttagatt ctgtgctttt aagcagagat tacaggctgg ttacgttacc agctgccggg
     9661 cctttagcgc cgctttcgat ggtgaaggac actttctgac cttcgtccag agatttgtaa
     9721 ccatcgttct ggatagcaga gaagtgtacg aacacgtctt tagagccatc gtcaggagtg
     9781 atgaagccga agcctttgtc agcgttgaac cattttacga taccagtcat tttaccggac
     9841 atagtgtatt acctttaata attaagtgtg cctttcggcg atatggcgtg ctttacagat
     9901 tttgaagcgt taaaggaatg tgcactacga ggggtatcaa cgataactct tgaagggact
     9961 tgccttacta cactgcttta atggtctgta cgtcaaaccg ttgatgtgca ttaagccacg
    10021 cattggcggg tgatgcaaca attatttttc atatttatga ttaatcggtt ggaaaacggt
    10081 cctgtcatca ggaccgtaaa cagcaataaa gtggataaaa gtctattcca tcaactgctt
    10141 acttaatgcc gggttggctt gaatcaaacg catcaatttt agttcggcac ttgaaggctt
    10201 cacgcgtctg gattcccatt cctttaccat ggcgactgat acgcccaaaa cccgggcgaa
    10261 atcatcgatt tttaaccctg tcccttttcg taactgctca atttcggtac aggacgttgt
    10321 tctgtgcgtc agggtaattt tctgcgtttc atctttaaaa acaatctgtt ccaggctgct
    10381 caacagctca tgcattggat ctttatattc cattgaaaac tcctcaaatc acactgcggg
    10441 atcgtgaatt gcatcgaagc tcattaagaa tagtcgggaa aagcagacac agcggggtga
    10501 agtcagggca tgctctggct atgattcgat tcagccagtc atttagcgtg acttatagcc
    10561 acctaacgat ctgataaatg ccgatatatc tcttaccact cctggtttgt aaagatttcg
    10621 aaaatctaac cttctgcgaa ggcgtaaatc tctggctaca aaaaaagcaa cggcactatc
    10681 attttttaac aatgaaacaa caaattaacc ttgtttcatt ttctccatga ttttaccagg
    10741 aagattacgt agaaagggta tcctgcaagc ctgtcctgga ctatccttgt cacgtcagac
    10801 acgcgtgtgt cgttgtgcgc tttttttggg tgaaaggagt aagaaaatgg cgacaggaaa
    10861 gtcctgctct cgctggtttg cgcctcttgc ggcgttatta atggtagtta gcctgagtgg
    10921 gtgttttgat aaagaaggcg atcagcgtaa agcgtttatc gacttcctgc agaatacagt
    10981 gatgcgtagc ggtgaacgtc taccaaccct gactgccgat cagaaaaaac agtttggtcc
    11041 ttttgtctct gattacgcga ttctgtatgg ttattctcag caggtgaatc aggcgatgga
    11101 ttccggtctg cgcccggtcg tagacagcgt taacgcaatt cgcgtaccgc aggattacgt
    11161 tacgcaaagc ggtccgctgc gtgaaatgaa cggttcgctg ggtgtgctgg cgcaacaact
    11221 gcaaaatgcg aagttgcagg ccgatgctgc gcactctgcg ctaaaacaga ccgatgattt
    11281 gaaaccggtc tttgatcagg cattcaccaa agtggtaaca acgccagctg atgcattgca
    11341 accgttgatt ccggctgcac aaactttcac gcaacaactg gtgatggttg gagactacat
    11401 tgctcaacaa ggtactcagg tgagctttgt agcaaatggt atccagttcc cgacttcgca
    11461 gcaggcaagt gaatataaca aactgattgc gccgttaccg gcacaacatc aggcgtttaa
    11521 tcaggcctgg actacagcag ttactgcaac ccaataaaga gtaaaagccc gagcgattat
    11581 cgggctttac ttcacgcagt cgcggcttag tccgcgacgt gcggattcac acagttcttc
    11641 tcaacctttc cttgtaacgc atcaatcaaa ttatccacgg cacaggcggc catgccataa
    11701 cgcgtctcat gggtggcaga tccaatatgc ggtactgcga cgacgttggc cattgagagc
    11761 aacggcgaat ctacggacag tggctcttgt tcgaagacat ccagcccggc ggcgtgaatt
    11821 tcgcctttct gcaatgcagc aatcagtgca ttttcatcaa ccaccgggcc acgtccggca
    11881 ttaatgaaaa tggcggagga tttcattttg gcgaattgtt ctgcgccaaa cagatgatgc
    11941 gtctcatcag ttaacggcag gatcaggcaa acgaaatctg actcttgtaa cagagtatcc
    12001 aaatcgcagt agcgggcgtt gaagcgttct tctgcttctt tatggtggcg gcgcgcgtta
    12061 tagaggatgg gcatgttgaa gccaaagtgc gcacgttgtg ccagcgccat gccgatccgt
    12121 cccatcccga caatgcccag tgttttatgg tgaacgtcag tgccgtacca gtccgggcct
    12181 atgctcgcgg tccattcgcc tgcttttacc cgttctgcta cctccacaac ccgacgagcg
    12241 gtagacaaca ccagcgccat cagcgtatcg gcgacggttt ctgttaatac ggttggcgtg
    12301 tgcatcagca gaatttttcg ggcggtaagc gcatcgacat caaaattgtc atagccgacg
    12361 gagatcgttg atgtggcacg cagtttcggc attttttcca gcaatgcggc atctacattc
    12421 tcgtttgaac ccagtaaacc ttcagcttcg gcaaaaattg ctgcattttg ttcgacggtt
    12481 tgtgggctga ggtttgccac ctggtgaacg gtgaaatgct cttgcaggcg ttgcagtaaa
    12541 tcatcaggta aggctttgta gaggataacg gacggcttca tgcttctctc cattcactga
    12601 taaggcgatc accttagcct gaaatatgat gaaggaaaac tgaagaatgc caggtgattt
    12661 ctgcatcacc tggcatgaaa gggattacag cgggcttaag gtaatttcta cacggcggtt
    12721 ttgcgcctta ccttctgcgg tgctgttgct ggcgattggg ttagccgggc caaggccctg
    12781 agtacggatg cggctggcgt ccacgccctg ggtgatcaac gcgctggcaa cggaatccgc
    12841 acgttgctgg gagagacgca tgttcaggtc gtgaccaccc gtgctgtcgg tataaccaat
    12901 cacgttaacc gccgttttcg gatactcttt cagcaccatt gccacgccgg tcagggtgtt
    12961 agcgcctgcc ggtttcaggg tcgcgctgct gctgtcgaag gtcacattgt tcggcatatt
    13021 gaggataatg ttatccccgc tgcgggttac gctaacacca gtgccgcgca ttttgtcacg
    13081 cagcttcgct tcctgcacat ccatgtaata acccacgccg ccgcccagag ctgcgcctgc
    13141 tgctgcgcca atcagcgcgc ctttaccgcg atctttcttc gaagaagaga gcgcaccaat
    13201 acccgcgccc acgagagagc ccagacctgc gccgatagca gatttgcctg cttcgcgttc
    13261 gccggtgtaa gggttagttg tgcagccaga taccgccaga gcgccactca ctacggcggc
    13321 aataagataa acacgtttct tcattgttaa tccttaataa cctttttatt ctttgccacg
    13381 ggttccgtgg cgggagatta tgccgcgtga acatgaagat tattcctggg aatactcgga
    13441 aatttgtaag taatatttaa ctgctcaata catctaacct ttcaggagtc ttcggtttgg
    13501 ccaactcatc ctcacgatat tccgttctga ctgccgccca ctgggggccc atgctggttg
    13561 aaaccgacgg caaaaccgtg tttagctcgc gtggcgcgtt agccacagga atggaaaact
    13621 ccttgcagag cgcggttcgc gaccaggttc acagcaatac gcgggtacga tttccaatgg
    13681 tgcgcaaagg ctttctcgcg tcaccggaaa acccgcaagg cattcgtggg caggatgaat
    13741 ttgttcgcgt gagttgggat gaggcgctgg atcttattca ccaacaacat aaacgcattc
    13801 gtgaggctta tggtccggca tcgatttttg ctggttccta cggctggcgt tcaaacggcg
    13861 tgctgcataa ggcctcgaca ttattacaac gctatatggc gctggcaggc ggttatatcg
    13921 ggcatctggg ggattattcg accggtgcgg cacaggcgat catgccgtat gtcgtgggtg
    13981 gcagcgaagt ttatcaacag caaaccagtt ggccgctggt gctggaacat agcgatgtcg
    14041 tggtgttgtg gagtgctaac ccactcaata cgctgaaaat tgcgtggaat gcatctgatg
    14101 agcaggggct ttcttacttt tctgcactgc gtgacagtgg gaaaaagctg atctgcattg
    14161 atccaatgcg atcggaaacc gtcgatttct ttggcgataa aatggagtgg gtggcaccgc
    14221 acatgggcac cgatgttgcg ctgatgctgg ggatcgccca tacgctggtg gaaaatggtt
    14281 ggcacgacga agcgtttctg gcgcgttgca ccacaggtta tgccgtcttc gcctcttatt
    14341 tgctgggcga gagtgacgga atagcaaaaa ccgccgaatg ggcagcagag atttgtggtg
    14401 ttggcgcagc gaaaatccgc gagctggcgg ctattttcca ccaaaatacc accatgctga
    14461 tggcaggctg gggaatgcag cgccaacagt ttggtgagca aaaacactgg atgatcgtca
    14521 cgctggcagc aatgttgggg caaatcggca cacccggcgg cggttttggt ctttcttacc
    14581 attttgccaa tggtggtaac cccacgcggc gttctgcggt gctctcttcc atgcagggca
    14641 gcttgccggg tggctgcgat gcggtggata aaatccctgt tgcccgcatt gttgaagcac
    14701 tggaaaaccc tggtggcgca tatcaacaca acggtatgaa ccgacatttc ccggatattc
    14761 gttttatctg gtgggcgggc ggtgccaact ttactcatca tcaggatacc aatcgtctga
    14821 tccgtgcctg gcaaaaaccg gagctggtgg tgatctctga atgcttctgg acggcggcgg
    14881 cgaaacacgc ggatatcgtt ctgcctgcga ctacctcttt tgagcgtaat gatctcacca
    14941 tgaccggtga ttacagtaat cagcatctgg tgccgatgaa gcaagtggtg ccgccacgct
    15001 atgaagcgcg taatgatttt gatgtttttg ccgagttaag tgaacgctgg gagaagggcg
    15061 gttatgcacg ttttacggaa ggaaaaagtg agctgcaatg gctggaaacg ttttataacg
    15121 ttgcccggca gcgcggggca agccagcagg ttgaattgcc gccatttgct gagttctggc
    15181 aagccaacca gttaattgag atgccggaaa acccggacag cgagcggttt attcgcttcg
    15241 ctgatttttg ccgcgatccg ctggcgcatc cgttaaaaac cgccagcggc aagattgaaa
    15301 tcttctcaca gcgtattgcc gattacggtt acccggattg ccctgggcat ccaatgtggc
    15361 tggagccgga cgaatggcag ggcaatgccg aaccagaaca gttgcaggta ctttctgccc
    15421 atccggcgca ccgcctgcac agccagctga attacagttc tctgcgcgaa ttgtacgcgg
    15481 tagcaaaccg tgagcctgtc accattcatc ctgacgatgc tcaggcgcgc ggcattcaag
    15541 atggcgatac tgttcggctg tggaacgcac gcgggcaaat tcttgccgga gcggtcatta
    15601 gcgagggaat taaacctggc gtgatttgta ttcacgaagg ggcatggccg gatctggatt
    15661 taaccgctga cggtatttgt aaaaacggcg cagtcaacgt gctgaccaaa gatctcccca
    15721 gctcgcggct ggggaatggc tgtgcgggta atacggcgct ggcatggctg gaaaaataca
    15781 acggtccgga actgacactt acagcgtttg aaccaccggc cagctcataa tccaggtggg
    15841 tagttgggtt tcatcctgcc atgcgcaatc gacaatgtga aaaccctgcg cacggtaaaa
    15901 atctatcgcc ggttgatttt tttgataaac ctccagcatc agatggggat agcgctgctg
    15961 cacatactgc atcagcgcct taccaatacc gcgtctgacg gccttcggtg cgacaaacat
    16021 tgctgccaga aatcggcctt ccataatgct gacaaaaccg agaagcttac cgtcctcttc
    16081 ccagacccag ttttgcgcgt tggcaagata ggcatcccgc accagcggaa tgcagtcacg
    16141 ccagtaattc gcttttataa agggatgccc ccaggttgta ctttccagcc acagttcgag
    16201 gatcgcgggg agttctgaac gttgcgcttc ccgaatcatg gtttatttcc cggatagcaa
    16261 cagcagccaa ccacatgatc attcaccagc ccacatgcct gcataaagga gtaacagatt
    16321 gtggtgccga caaacttaaa accacgtttt ttcagtgcct tagatagggc gtcggaggcg
    16381 gacgtagatg tgggaatttc gctcaacgtt gtggcttgtg tcacctgtgg ctgatgattt
    16441 acaaacgacc agacaaagtc ggcaaacggt tcgccgttct gttccatttg caggtacgcc
    16501 cgcgcattac caataattgc ctgaattttc cctcgatggc ggataatccc ggcgtcctgt
    16561 accagtcttt cgacatcctc ttcctgcatt gctgcgacct tcaccggatc gaactgatga
    16621 aagcaggcgc gatagttttc gcgttttttg aggacggtga tccacgataa cccagcctgc
    16681 tgcccttcaa ggcagatcat ttcgaacagt tttttactgt cagtttcagg cacgccccat
    16741 tcattatcat ggtaggcaat ataaagcggg tcctgactca cccagccgca acgttccata
    16801 ctttccctcg ctatgtaccc gatcgtaaaa atttcagccg cttttcttga cgcatcggtg
    16861 aaaaagtcaa tatttaatac agggctacta tgcccgatat ccttcatgca atgacaataa
    16921 tattgccgaa ttcggctctt ccctggagtc gtgttaatga tcataaaaaa aagcggtggt
    16981 cgctggcagc taagcctgct ggcgagcgtg gtaatcagtg ccttttttct caacacagct
    17041 tacgcctggc aacaagaata tatcgttgat acgcaacccg gacattccac agagcgttac
    17101 acctgggata gtgatcatca acctgattac aacgatattt tgtcgcaacg tattcaaagt
    17161 agccaaaggg cgctgggact ggaagtcaat ctggcggaag aaactcctgt ggatgtgacc
    17221 agcagtatga gtatgggctg gaattttcct ttatatgaac aggttacaac cggcccggtc
    17281 gcggcattac attacgatgg cacaaccacc tcgatgtata acgagtttgg cgacagtact
    17341 accacgctga ccgatccgtt atggcatgcc agcgtgagta gcttaggctg gcgtgttgac
    17401 tcccggcttg gcgatctccg accctgggcg caaatcagct ataaccagca atttggcgag
    17461 aatatctgga aggcgcaatc aggcctgagc cggatgacgg cgacaaacca gaacggcaac
    17521 tggctggatg tcaccgtagg cgctgatatg ttgctcaatc aaaatattgc cgcctatgcc
    17581 gcgctaactc aggcagaaaa taccactaat aatagcgact atctgtatac gatgggggtt
    17641 agcgccagat tttaacgtaa cagtcacaat tgaaaccatt gaataacaac agttgtggcg
    17701 atagtgggtg ctaacttacc aaataataaa tttggtgaat aattgtcgcg tcattcattc
    17761 ctgaactaag gcatttcatt ccgttctgat ggcatttcat gccgtttttc cccaggcata
    17821 aaatgcactt cgttatggtt gtcggcagag atttttcctt tttattactg caggaatact
    17881 gccatgacac cttcaaatta tcagcgtacc cgctggctga cactcatcgg tactatcatt
    17941 acccagtttg cactggggtc ggtttatacc tggagcctgt ttaatggcgc gctttctgcc
    18001 aagctggatg cgccggtaag ccaggtcgct ttctctttcg gcttgttaag tctggggctg
    18061 gcaatttcgt cttctgttgc gggcaaattg caggaacgtt ttggcgttaa acgcgtcacc
    18121 atggcttccg gcattttgct gggattaggc ttcttcctga ccgcgcattc caacaacctg
    18181 atgatgctgt ggttaagcgc cggtgtgctg gtcggtctgg cggatggcgc gggttacctg
    18241 ctgacgctct ctaactgcgt gaagtggttc ccggagcgta aaggtctgat ctccgcgttc
    18301 gctatcggtt cttatggtct gggtagcctg ggtttcaaat ttatcgacac gcagctgctc
    18361 gaaacggtgg gtttggaaaa aacatttgtg atttggggag cgattgcgct ggtgatgatt
    18421 gttttcggcg caacgttaat gaaagacgca ccaaaacagg aagtgaaaac cagcaatggt
    18481 gtggtggaga aagattacac gctggcagag tcgatgcgta aaccgcagta ctggatgtta
    18541 gcggtaatgt tcctgactgc ctgcatgagc ggcctgtacg tgattggggt agcgaaagat
    18601 atcgcccaaa gtctggcaca ccttgatgtg gtttccgcag ctaatgcagt cactgttatt
    18661 tccatcgcca acctttcagg tcgtctggtt ctggggatac tgtctgacaa aatcgcccgt
    18721 atccgtgtta tcaccattgg tcaggtgatt tcgctagtgg gtatggcggc cctgctgttt
    18781 gcaccactga atgcagtgac gttctttgca gcgattgcct gcgtggcatt taactttggc
    18841 ggcactatta ccgtctttcc gtcactggtc agtgagttct tcggcctcaa taacctggcg
    18901 aaaaactacg gtgtgattta cctcggcttc ggtatcggta gcatttttgg ttcgattatc
    18961 gcctcactgt ttggcggttt ctatgtgacc ttctacgtga ttttcgccct gctgattctg
    19021 tcattggcgc tttctacgac gattcgtcag ccagagcaga aaatgttgcg tgaggcgcat
    19081 ggctcccttt aatgctcagc cgcgagctac tgggcttcag cttccgtgtg catcaaacgc
    19141 atcaacgaaa tgtataaaca atgactcgta aaatttgggc cagttaactg gcccttttta
    19201 ctgctttttg taacgctaaa gtctcttttc aaacttgcat ttttgtaaat ttgtgcttca
    19261 tgcacactct ttccccacac tttttcgctt tgctgtggtc tacttattcg cgcgtgtaga
    19321 ttttacttat ctgactacct ccgcactttt cccctgccgg gcctgaaaag ccgctaagca
    19381 gggtgttatc acctgtttgt ccagggtttg tttgcatgag atacatcaaa tcgattacac
    19441 agcagaagct gagctttttg cttgcaatct atattggcct ttttatgaat ggcgcggttt
    19501 tttatcgccg cttcggcagc tatgcgcacg attttaccgt ctggaaaggc gtttctgctg
    19561 ttgttgaact ggccgccacc gtactggtga ccttcttttt actacgtctt ctatcgctgt
    19621 ttggccgccg cagctggcgt attctggcgt cgctggtggt gctcttttcc gcaggtgcca
    19681 gctattacat gaccttcctt aatgtggtca ttggttatgg catcatcgct tccgtcatga
    19741 ccaccgatat cgacctgtca aaagaagttg ttggtctgaa ctttattctc tggttaatcg
    19801 ccgttagtgc attgcctctt atccttatct ggaataaccg ctgtcgctac accttgctcc
    19861 gacaactgcg aaccccaggg cagcgtattc gcagcctggc ggtcgtcgta ctggcgggta
    19921 ttatggtttg ggcaccgatt cgtttgctgg atatccagca gaagaaagtg gagagggcga
    19981 ccggcgttga tttgccgagt tatggcggtg tcgtagcgaa ctcttatctg ccatcaaact
    20041 ggctttctgc gttggggctg tatgcctggg cgcgggtcga tgaatcttcc gataataatt
    20101 cattgcttaa tccggcgaag aaattcactt atcaggcacc gcaaaacgtt gatgacactt
    20161 atgtcgtgtt tatcatcggt gaaaccacgc gttgggacca tatgggcatt ttcggctatg
    20221 agcgtaatac cacgccgaaa ctggcccagg agaaaaatct ggcggcgttc cgtggttact
    20281 cctgtgatac cgcaaccaaa ctctcactgc gttgcatgtt tgtacgtcag gggggcgcgg
    20341 aagataatcc gcagcgcaca ttaaaagaac agaacatttt cgcggttctg aagcagttag
    20401 gattcagttc tgacctctac gctatgcaga gcgaaatgtg gttctacagc aacacgatgg
    20461 cggacaacat tgcttatcgt gagcagattg gtgcggagcc acgtaatcgt ggcaagccgg
    20521 tagatgatat gttgctggta gacgaaatgc agcaatcgct agggcgcaac ccggatggta
    20581 agcatctgat cattctgcat accaaaggtt cgcattttaa ctacacccag cgttatccgc
    20641 gtagcttcgc gcagtggaag ccggaatgta ttggtgttga tagcggctgt accaaagcgc
    20701 agatgatcaa ctcctatgac aactcggtga cctatgtgga tcactttatc tccagcgtga
    20761 ttgatcaggt tcgcgataag aaagcgattg tgttctacgc agctgaccac ggtgagtcaa
    20821 ttaatgaacg cgagcacctg cacggcacgc cgcgtgaact ggcaccgccg gagcagttcc
    20881 gcgtaccgat gatggtctgg atgtcagata aatatctgga aaatccggcc aatgctcagg
    20941 catttgcgca gctgaaaaaa gaagccgaca tgaaagtgcc acgccgtcat gtagagctgt
    21001 acgacaccat catgggttgt cttggctata cttcaccgga tggtggaatt aacgaaaaca
    21061 acaactggtg tcacatcccg cagacaaaag tggcagcggc taactaagcg gcctgctgac
    21121 tttctcgccg atcaaaaggc attttgctat taagggattg acgagggcgt atctgcgcag
    21181 taagatgcgc cccgcattcg gtgattggcg cagcctggta gcgcacttcg ttcgggacga
    21241 aggggtcgga ggttcgaatc ctctatcacc gaccaaattc gaaaagcctg ctcagcgagc
    21301 aggctttttt gcatttgaag cccatgagga tgagaacctc cgggggcggg gaggttcgac
    21361 tcgagcgaag cgagagaacg ttgcgccagc aacggcccgc agggcgagcc acgaagtggc
    21421 gagtaatcct ctatcaccga ccaaattcga aaagcctgct cagcgatcag gctttttttg
    21481 catttgtagc ccatgaggat gagaacctcc gggggcaggg aggttcgact cgacgtaggc
    21541 ctgataagac gcgccagcgt cgcatcaggc aacggcaccg cacttattgt cggatgcggc
    21601 gcgaccgcct tattcgacca acacaccaca cattttcctg ccaactttgc gccccatatc
    21661 tcactatcca ttaacggatt tgtgacagaa accattgtct ttttttatat ttgcttaaat
    21721 ctttttttgc gttacttatg gctggcgctg gcattttccg ctgtgcgttt tagcgttcat
    21781 ggtattaatc gctcagatat ccaccttttc ctcagatttt tttcattaaa tgcataaaac
    21841 ttaatcacca aatgttgcta aatattaagc tgattgttct ggtcatgttg gagtagcaca
    21901 aatttctctg ctgcaaatag ctgtttgaag tttttttaat ctttgtttgt ggattctcac
    21961 cgttggtgta aatcccggtt ggctgtattg acgttttcac attctgttga cagattgtag
    22021 gtcacgaggg gcattttatg gaggatccgc actgttacac tgatgttaat tagtacggca
    22081 tccccacctc ataacgttga cccgaccggg caaaaaacaa aaaaggtcag gcagcgacaa
    22141 cccactgcaa agggttaaaa caacaaacat cacaattgga gcagaataat gcgtatttcc
    22201 ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg tggctatgac cgtcgcagca
    22261 agtgttcagg ctaaaactct ggtttattgc tcagaaggat ctccggaagg gtttaacccg
    22321 cagctgttta cctccggcac cacctatgac gcctcttccg taccgcttta taaccgcctg
    22381 gttgaattta aaatcggcac cactgaagtg atcccgggcc tcgctgaaaa gtgggaagtc
    22441 agcgaagacg gtaaaaccta taccttccat ctgcgtaaag gtgtgaagtg gcatgacaat
    22501 aaagaattca aaccgacgcg cgaactgaac gccgatgacg tggtgttctc gttcgatcgt
    22561 cagaaaaacg cgcaaaaccc gtaccataaa gtttctggcg gtagctacga atacttcgaa
    22621 ggcatgggct tgccggagct gatcagcgaa gtgaaaaagg tggacgacaa cacagttcag
    22681 ttcgtgctga ctcgcccgga agcgccgttc ctcgctgatc tggcaatgga cttcgcctct
    22741 attctgtcaa aagaatatgc tgatgcgatg atgaaagccg gtacaccgga aaaactggac
    22801 ctcaacccaa tcggaaccgg tccattccag ttacagcagt accaaaaaga ttcccgtatc
    22861 cgctacaaag cgtttgatgg ctactggggc accaaaccgc agatcgatac gctggtcttc
    22921 tctattaccc ctgatgcttc cgtgcgttac gcgaaattgc agaagaatga atgccaggtg
    22981 atgccgtacc cgaacccggc agatatcgct cgcatgaagc aggataaatc catcaacctg
    23041 atggaaatgc cggggctgaa cgtcggctac ctctcgtata acgtgcagaa aaaaccgctg
    23101 gatgacgtga aagttcgcca ggcactgacc tacgcggtga acaaagacgc gatcatcaaa
    23161 gcggtttatc agggcgcggg tgtatcagcg aaaaacctga tcccgccaac catgtggggc
    23221 tataacgacg acgttcagga ttacacctac gatcctgaaa aagcgaaagc cttgctgaaa
    23281 gaagcgggtc tggaaaaagg tttctccatc gacctgtggg cgatgccggt acaacgtccg
    23341 tataacccga acgctcgccg catggcggag atgattcagg cagactgggc gaaagtgggc
    23401 gtgcaggcca aaatcgtcac ctacgaatgg ggtgagtacc tcaagcgtgc gaaagatggc
    23461 gaacatcaga cggtaatgat gggctggact ggcgataacg gggacccgga taacttcttc
    23521 gccaccctgt tcagctgcgc cgcctctgaa caaggttcta actactcaaa atggtgctac
    23581 aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg acgaccataa caaacgcgtt
    23641 gaactgtaca agcaagcgca ggtggtgatg cacgatcagg ctccggcact gatcatcgct
    23701 cactccaccg tgtttgaacc ggtacgcaaa gaagtcaaag gctatgtggt tgatccatta
    23761 ggcaaacatc acttcgaaaa cgtctctatc gaataattaa aagccataca agactgatgg
    23821 caaaggcaaa actgcctgat gtgctccgct tatcaggcct acgaaaattc tgcaatgtat
    23881 tgaatttgca cgattttgta ggccggataa ggcgttaacg ccgcatccgg cataaacaaa
    23941 gcgcactttg tcaacaatct gtatacccgg tggcgctgtg cctctgcgtg cagcgccatc
    24001 cggcagcaat acttcattct ctgtccgaca agggcgggaa ttgatttgtg agcaatacag
    24061 acacgcagtt ccaggctgcg ggtcactata gagaatccgg gttatgttgc agtttattct
    24121 ccgacgtttg ggactcgtca tccccacgtt tatcggtatt acccttctca catttgcctt
    24181 tgtccacatg atcccgggcg atccggtgat gatcatggca ggcgaacgtg ggatctcccc
    24241 tgagcgtcac gcgcagctgc tggctgaact cggcttagat aaaccgatgt ggcagcagta
    24301 tctccattac atttggggcg taatgcacgg cgatctaggc atttcaatga aaagccgaat
    24361 tccagtatgg gaagagttcg tgccgcgctt tcaggccacg ctggaacttg gcgtctgcgc
    24421 gatgattttt gctacggcag tcggtattcc ggttggtgtg ctggctgcgg ttaaacgcgg
    24481 ttccattttc gatcacacag cggttggcct ggcgctgacc ggctactcga tgccgatctt
    24541 ctggtggggc atgatgctga tcatgctggt ttcggtgcac tggaacctga cgcccgtctc
    24601 cggtcgcgtg agcgatatgg tgttcctcga tgactccaat ccgttaaccg gttttatgct
    24661 aatcgacacc gccatctggg gtgaagacgg caactttatc gatgccgtcg cccatatgat
    24721 cttgcctgcc attgtgctgg gtactattcc gctggcggtc attgtgcgta tgacacgctc
    24781 ctcgatgctg gaagtgctgg gcgaggatta catccgcacc gcgcgcgcca aagggctaac
    24841 ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg atgctgccgg tggtgaccgt
    24901 tatcggcctg caggtgggaa cattgctggc gggggcgatt ctgaccgaaa ccatcttctc
    24961 gtggcccggt ctgggacgct ggttgattga cgcactgcaa cgccgcgact atccggtagt
    25021 gcagggcggc gtattgctgg tggcgacgat gattatcctc gtcaacttgc tggtcgatct
    25081 gctgtacggc gtggtgaacc cgcgtattcg tcataagaag taaggggaca tcatgtcaca
    25141 ggttactgaa aataaagtga ttagcgcacc ggtgccgatg accccgttac aggagttctg
    25201 gcactatttt aaacgcaaca aaggcgcggt cgtcgggctg gtttacgtcg tcatcgtgct
    25261 gttcatcgcg atctttgcca actggattgc accctataac ccggcggaac agttccgcga
    25321 tgcactgctc gccccgccag cctggcagga aggcggcagc atggcgcact tgctgggcac
    25381 cgatgacgta ggccgtgatg tgctgtcgcg cctgatgtac ggtgcgcgcc tgtcgctgct
    25441 ggttggctgt ctggtagttg tgttatcgct gattatgggc gttattctcg gcctgatcgc
    25501 cggttacttt ggcggcctgg tcgataacat cattatgcgc gtggtcgata tcatgctggc
    25561 gctgccaagt ctgctgctgg cgctggtgct ggtggcaatt ttcggcccgt cgattggtaa
    25621 cgccgcgctg gcactgacct tcgttgcctt gccgcactat gtgcgcttaa cccgcgccgc
    25681 cgtgctggtg gaagttaacc gcgattacgt caccgcgtct cgcgtggcgg gtgccggggc
    25741 gatgcgtcag atgtttatta acatcttccc gaactgcctt gcgccgctga ttgttcaggc
    25801 gtcgctcggt ttctctaacg ccattctcga tatggctgct cttggtttcc tcggcatggg
    25861 ggcacagccg ccaacgcctg agtggggcac catgctctcc gacgtgttgc agttcgcgca
    25921 aagcgcctgg tgggtcgtga ccttcccggg tctggcgatc ctgctgacgg tgctggcatt
    25981 taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc aaactgaagc agtaagaggt
    26041 tcgagatggc gttattaaat gtagataaat tatcggtgca tttcggcgac gaaagcgcgc
    26101 cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca gggcgaagtg gtcgggattg
    26161 tgggtgagtc cggctccggt aagtcggtca gttcactggc gattatgggg ctgattgatt
    26221 atccgggccg cgtaatggca gaaaaactgg agtttaacgg ccaggatttg cagcgtatct
    26281 cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc gatgatcttc caggacccga
    26341 tgaccagcct taacccgtgc tacaccgtgg gtttccagat catggaagcg attaaggtgc
    26401 atcagggcgg taataagagc actcgccgtc agcgggcgat tgacctgctg aatcaggtcg
    26461 gtattcccga tccggcatcg cgtctggatg tttacccgca tcagctttcc ggcggcatga
    26521 gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc aaaactgctg attgccgatg
    26581 aaccgaccac cgcgctggac gtgaccattc aggcgcaaat catcgaacta ctgctggagc
    26641 tacagcagaa agagaacatg gcgctggtgt taattaccca tgacctggcg ctggtggcgg
    26701 aagcggcaca taaaatcatc gtgatgtatg caggccaggt ggtggaaacc ggtgatgcgc
    26761 acgccatctt ccatgcgccg cgtcacccgt atactcaggc attgctgcgt gcgctgccag
    26821 aatttgctca ggacaaagaa cgtctggcgt cgttgccagg tgtcgttccc ggtaagtacg
    26881 accgcccgaa cggctgcctg cttaacccgc gctgccccta tgccactgac agatgtcgcg
    26941 ctgaagaacc ggcgctgaat atgctcgctg atgggcgtca gtccaaatgc cattacccac
    27001 ttgatgatgc cgggaggccg acactatgag tacgcaagag gccacctcgc aacaaccgct
    27061 gttgcaggct atcgacctga aaaaacatta tccggtgaag aaaggcatgt tcgcgccgga
    27121 acgtctggtg aaggcgctgg acggcgtttc gtttaacctt gaacgtggca aaacgctggc
    27181 agtagtgggc gaatctggct gcggtaaatc gaccctcggt cggttgctga cgatgatcga
    27241 aacgcccacc ggtggtgagc tgtattacca ggggcaggat ctgctcaagc acgatccgca
    27301 ggcgcagaag ctgcgtcggc agaaaatcca gattgtcttc cagaaccctt acggttcgct
    27361 gaatccgcgt aaaaaagttg ggcaaattct cgaagagccg ctgcttatta acaccagctt
    27421 cagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg gcgaaagtcg gcctgaaaac
    27481 cgagcactat gaccgctatc cgcatatgtt ctccggcgga caacgtcagc gtatcgctat
    27541 tgcccgtggt ctgatgctcg acccggacgt ggtgattgcc gatgaaccgg tttctgcgct
    27601 ggacgtctca gtgcgtgcgc aggtgctgaa tctgatgatg gatttgcagc aggagttggg
    27661 gctttcttat gtctttatct cccacgacct gtcggttgtg gagcacattg ctgatgaagt
    27721 gatggtgatg tacctgggcc gctgcgtgga gaaaggaacg aaggaccaaa tcttcaataa
    27781 cccgcgccat ccgtacaccc aggcgctgct ctccgcgacg ccacgcctga acccggacga
    27841 tcgtcgtgaa cgcatcaaac tcaccggtga actgccaagc ccgctgaatc ctccgccggg
    27901 ttgcgccttc aacgcccgct gtcgtcggcg cttcggcccc tgcacccagt tgcagccgca
    27961 gctaaaagac tacggcggtc aactggtagc ttgttttgct gttgatcagg atgaaaatcc
    28021 gcagtgttaa ctcacaaaac cggggccatg cgctccggtt tactcaatca gcttcaacag
    28081 tggcgacaag catagcaaaa tgccgtaaag cagaattagc caggctttaa atccgcgaag
    28141 tttttccagt tgtgcgactt tatagatcag gaaaaacgga atgaggcacg acacaattcc
    28201 atataacggg cttcccaact gaaagaacac cagcaccgat acacgaaacg aaacccaaat
    28261 cgtcaatgta atgacgataa aagcgcagat cgccagagtc agcatgcgtg agttaatttt
    28321 cttggtatca ataattcggc ttaacagatt gagaataatg cctttaatag cctcgtggaa
    28381 accgagataa atgccaaaga atgcggtcag tacggcaaag atattcagca ccgtagaggt
    28441 gatatgaatg atatgtccag ggatcacctg cgcggccagc gccagtgccg agatattttg
    28501 ttcaaaggca gaaacggctt cttcgtggct aattgagaag gtaaacgaaa aggcaaaaaa
    28561 caggatcacc gcgatgagcg tgatataact aattcggtgg gtacgcagcg cgagccgggt
    28621 tgccagtact ttatctgctt cccgtttacg ataggcaata ttcattgggt taagtacctg
    28681 aataaatacc gcagaaaaga agcaaaatgg aattgtaagc aggacatcgc ggaaaaagac
    28741 ggaggcttgc gggaaggcgg ttatattggc gaaattccag tgcgggatca tcgcaaaacc
    28801 gaacacgaca ataatcccta ctttgaccac caccattggc ccggaaatct taaacagtaa
    28861 tcgttcacca ccagacgcaa tcgccaccag tacggcgaaa atagcgactt tataaagtag
    28921 agattgtgaa agatcggcat cggttaaacc gaaggttttc aggtacgagg cgctgtcgaa
    28981 aaccacggag agagagtaga taaaaatccc gtggataatc atcaaaaagt agataacccc
    29041 gaggaaaatt ccccagttct tccccaggta atgactgata atatcggtgt agtcattaca
    29101 ggaatcgctt tcagaaaggg tttttaaata aatgtcctgc actacccagg tggcaggata
    29161 agcaatgatc gctgcggtaa taaataccca aattcccttc aagccaattt gtactggcat
    29221 cagcacggtt ccggcaccaa tcgccatgcc tatgcataat aaaacccagc cgaagtcgta
    29281 gcgtgtaaac ggcaatttct gattgtgttt cgatagtgtg ttgtgctgca taaagtcctg
    29341 tccagaaaga agtcggcctc gcaaaaatga ataaacccag tttctcttgc gcaacaggtg
    29401 gagaagtcga atacgcagta tgtaaaaaat gaggcaacag attcactggg gaggtcgcga
    29461 ggaggcgcaa ttctacaaaa ttgcgcattt cactaccagt ctggagtcag cccggatatg
    29521 aactgcaact attgcaaata aagaaattaa gctggaaggc atcgcgcaat gaggaagatt
    29581 gccgggtgct gacagtaaaa agcaatcgta aaatcagcgg caagccggaa gggttccggt
    29641 gaggcgcaat gttgcggggg ctttatccct ggtggcattg gttgctggag agagaaaacc
    29701 cccgcacgtt gcaggtatat acctgacaac ataacggggg ctaatcttga acctagac
//
LOCUS       DAMZET010000039        29366 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.039, whole genome shotgun sequence.
ACCESSION   DAMZET010000039 DAMZET010000000
VERSION     DAMZET010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.039"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..838
                     /gene="menA"
                     /locus_tag="POB34_001834"
     CDS             <1..838
                     /gene="menA"
                     /locus_tag="POB34_001834"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDI5918799.1"
                     /translation="GTALAWWQGHFDPLVALLALITAGLLQILSNLANDYGDAVKGSD
                     KPDRIGPLRGMQKGVITQQEMKRALIITVVLICLSGLALVAVACHTLADFVGFLILGG
                     LSIIAAITYTVGNRPYGYIGLGDISVLVFFGWLSVMGSWYLQAHTLIPALILPATACG
                     LLATAVLNINNLRDINSDRENGKNTLVVRLGEVNARRYHACLLMGSLVCLALFNLFSL
                     HSLWGWLFLLAAPLLVKQARYVMREMDPVAMRPMLERTVKGALLTNLLFVLGIFLSQW
                     AA"
     gene            931..1416
                     /gene="rraA"
                     /locus_tag="POB34_001835"
     CDS             931..1416
                     /gene="rraA"
                     /locus_tag="POB34_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HDI5918800.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(1501..1746)
                     /gene="zapB"
                     /locus_tag="POB34_001836"
     CDS             complement(1501..1746)
                     /gene="zapB"
                     /locus_tag="POB34_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HDI5918801.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            2172..3017
                     /gene="glpF"
                     /locus_tag="POB34_001837"
     CDS             2172..3017
                     /gene="glpF"
                     /locus_tag="POB34_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HDI5918802.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            3040..4548
                     /gene="glpK"
                     /locus_tag="POB34_001838"
     CDS             3040..4548
                     /gene="glpK"
                     /locus_tag="POB34_001838"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDI5918803.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHALDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            4681..5691
                     /gene="glpX"
                     /locus_tag="POB34_001839"
     CDS             4681..5691
                     /gene="glpX"
                     /locus_tag="POB34_001839"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDI5918804.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            5788..6534
                     /gene="fpr"
                     /locus_tag="POB34_001840"
     CDS             5788..6534
                     /gene="fpr"
                     /locus_tag="POB34_001840"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HDI5918805.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(6539..6967)
                     /gene="uspD"
                     /locus_tag="POB34_001841"
     CDS             complement(6539..6967)
                     /gene="uspD"
                     /locus_tag="POB34_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HDI5918806.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(6994..7293)
                     /locus_tag="POB34_001842"
     CDS             complement(6994..7293)
                     /locus_tag="POB34_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HDI5918807.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(7505..7945)
                     /locus_tag="POB34_001843"
     CDS             complement(7505..7945)
                     /locus_tag="POB34_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDI5918808.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAANWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            8046..8645
                     /locus_tag="POB34_001844"
     CDS             8046..8645
                     /locus_tag="POB34_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HDI5918809.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            8753..9520
                     /gene="tpiA"
                     /locus_tag="POB34_001845"
     CDS             8753..9520
                     /gene="tpiA"
                     /locus_tag="POB34_001845"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDI5918810.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(9575..10330)
                     /gene="cdh"
                     /locus_tag="POB34_001846"
     CDS             complement(9575..10330)
                     /gene="cdh"
                     /locus_tag="POB34_001846"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HDI5918811.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVRKQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(10437..11426)
                     /gene="sbp"
                     /locus_tag="POB34_001847"
     CDS             complement(10437..11426)
                     /gene="sbp"
                     /locus_tag="POB34_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HDI5918812.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEVLLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(11746..12708)
                     /gene="pfkA"
                     /locus_tag="POB34_001848"
     CDS             complement(11746..12708)
                     /gene="pfkA"
                     /locus_tag="POB34_001848"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDI5918813.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(12889..13791)
                     /gene="fieF"
                     /locus_tag="POB34_001849"
     CDS             complement(12889..13791)
                     /gene="fieF"
                     /locus_tag="POB34_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HDI5918814.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            14079..14480
                     /locus_tag="POB34_001850"
     CDS             14079..14480
                     /locus_tag="POB34_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918815.1"
                     /translation="MATLKSVMAIATLIISLNANANDIKPLQKQLKPWQPLEITNKNN
                     SLTVVLPGKEISPDAYNNLIMTGVCTPVWTKDTPVSYLKKIKEISIVNQYKSIGYVFS
                     EPLKTCNEIGSLTPKQGQVLLSSQTRLYTGK"
     gene            complement(14532..14750)
                     /gene="ogrK"
                     /locus_tag="POB34_001851"
     CDS             complement(14532..14750)
                     /gene="ogrK"
                     /locus_tag="POB34_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HDI5918816.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(14832..15995)
                     /locus_tag="POB34_001852"
     CDS             complement(14832..15995)
                     /locus_tag="POB34_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HDI5918817.1"
                     /translation="MLDAMTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFAMVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDTTLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQITITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            complement(15995..16474)
                     /locus_tag="POB34_001853"
     CDS             complement(15995..16474)
                     /locus_tag="POB34_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI5918818.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISKVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESFIGKAGSMATKFTGMTGAG"
     gene            complement(16489..18936)
                     /locus_tag="POB34_001854"
     CDS             complement(16489..18936)
                     /locus_tag="POB34_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDI5918819.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRTASKSLSGDIRETQKSLRE
                     LNGQASRIEGFRKTSAQLAVTGQELKKARQEAAALAVQFKNTERPTNAQAKAMEAARK
                     NASELQAKYNSLRLSVQRQRQELSQAGINTRNLAHDERGLKNRISETTTQLNRQRDAL
                     ARVSVQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTMAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSAEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPIFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTAASTGKLDKLTAAFKASDGKTAELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQED
                     ALRNLTQTATKYVLKLDGWIQKNKSLASTIGLIAGGALALTGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFKNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESGTLDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTSGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            complement(18929..19048)
                     /locus_tag="POB34_001855"
     CDS             complement(18929..19048)
                     /locus_tag="POB34_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HDI5918820.1"
                     /translation="MADVAVIFHWPPSELYPMSLTELITWREKALRRSGNTNE"
     gene            complement(19081..19356)
                     /locus_tag="POB34_001856"
     CDS             complement(19081..19356)
                     /locus_tag="POB34_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDI5918821.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQITLMKPSAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(19413..19931)
                     /locus_tag="POB34_001857"
     CDS             complement(19413..19931)
                     /locus_tag="POB34_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HDI5918822.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(19944..20309)
                     /locus_tag="POB34_001858"
                     /pseudo
     CDS             complement(19944..20309)
                     /locus_tag="POB34_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(20311..21285)
                     /locus_tag="POB34_001859"
                     /pseudo
     CDS             complement(20311..21285)
                     /locus_tag="POB34_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
     gene            complement(21344..22198)
                     /locus_tag="POB34_001860"
     CDS             complement(21344..22198)
                     /locus_tag="POB34_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HDI5918823.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEIETAFSALKQEVTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            22372..24144
                     /locus_tag="POB34_001861"
     CDS             22372..24144
                     /locus_tag="POB34_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HDI5918824.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNVANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            24144..25178
                     /locus_tag="POB34_001862"
     CDS             24144..25178
                     /locus_tag="POB34_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDI5918825.1"
                     /translation="MSKKKGKTPRPAAKTITASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
     gene            25573..26556
                     /gene="dcm"
                     /locus_tag="POB34_001863"
     CDS             25573..26556
                     /gene="dcm"
                     /locus_tag="POB34_001863"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007796325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HDI5918826.1"
                     /translation="MPKAVSLFSGCGGSDAGLSKAGFDIVMANDILPYAREVYLANLP
                     ETDYLIQDIREVEKFPSADLLVGCYPCQGFSQGGARLADRSINFLYKEFARALTNIQP
                     KAFIVENVSGMRNSTFKHLLDDQIKRFSNAGNIGYKVVWNILKAHHYGVPQERKRLII
                     VGIRNDFDLEYKFPEATHGEEKSLPYVTIGEALKGLPEWPIGEFCDDPFHWYYLSRNR
                     RRNWDEVSKTIVSNLRHIPLHPISPELKKVGLDKWEFITNSPARRFSYKEAAILQGFS
                     KEYTNHGGYLTFPDMDSFGAARMLKERYKVVGNAVPPPLFEAVVRNIPDIW"
     gene            complement(26564..27520)
                     /locus_tag="POB34_001864"
     CDS             complement(26564..27520)
                     /locus_tag="POB34_001864"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918827.1"
                     /translation="MNYGLRSKMQGFATDADQAEFNALLDVSATIDSGIEDDKIAGTI
                     YDELENLDGHLEEEWMMEDQGKELLVGQIAEHIQWRAKVLDDMYPFEYKENHLTLKES
                     PSLVYLFCLCVSVINNLTKGDNVKLPRFFEVMAGRLFTKFFSSHAKHMHTGWPRSNGN
                     PSSYKELAYKLNSSISPNTREWSWRVENGLRDEDALRIKDCGVDFVTWVDFLDGRDGR
                     LFALGQCACGNDWPTKFQDIKIERLTPWFHPLTYIKCVKVFSTPYVLVDEMIREASAE
                     AGIIFDRVRLTIAYERFKDEFGDMQDELDALITFCKELKKAQ"
     gene            complement(27523..28698)
                     /locus_tag="POB34_001865"
     CDS             complement(27523..28698)
                     /locus_tag="POB34_001865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918828.1"
                     /translation="MKKNSVGVEDKLKHKAVQYINIEDLELDVENPRFGEDSSSSDTQ
                     VDVLNNIVKNYGVTDVISSIAVNGYFSAEPMVVRKNNNKKFTVMEGNRRLAACLILKN
                     DDRARDQVNLHQQYIDKYNSHGCPKIDPVPAIVFEDDDGVDKKSLISYLGVRHIVSTK
                     DWDSYAKAAWISRTIKEGDMSVSDISTMIGDRNSTIKRLLSGYNFIKQMESAGKYNKD
                     DSVKKGRGSNTSYPFSWVYTLLSYKSIQDFVGLSDNPTDPNPIDEKKLDNAKLLMTAM
                     FGNKNKGQNSQVKDSRNLGVLAEIVASPEKVILLKQGKDVEDINDLTQPIGDRLTSLM
                     LEIRSKLDECLTRVGREDLPMQDAIQLNIYLEQISKQFRSFRVSINEAITGSDSEGF"
     gene            complement(28895..>29366)
                     /locus_tag="POB34_001866"
     CDS             complement(28895..>29366)
                     /locus_tag="POB34_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HDI5918829.1"
                     /translation="PLTLKSGIAAPRSPVNNCGKLIGGDTELPAPTPSEHAAAVLNLV
                     DSGVIEWNDTEVVRALRGALKHGLRTPNRQQRNGSPLKAHEIAPSARLTRSERMQITR
                     IRVDLAQNGIRPQRWELEALARGATVNYDGKHFTYQVADEWLGFPSVDFFDCGA"
BASE COUNT         7166 a         7765 c         7086 g         7349 t
ORIGIN      
        1 cgggacggcg ctggcatggt ggcaaggtca cttcgatccg ctggtcgccc tgctggcact
       61 aattaccgcc gggctattac agatcctttc taacctcgcc aatgattacg gcgatgcggt
      121 aaaaggcagc gataaacctg accgcattgg gccgctacgc ggcatgcaaa aaggggtcat
      181 tacccagcaa gagatgaaac gggcgctcat tattaccgtc gtgctcatct gtctctccgg
      241 gctggcactg gttgcagtgg catgccatac gctggccgat tttgtcggtt tcctgattct
      301 tggcgggttg tcgatcattg ccgctatcac ctacaccgtg ggcaatcgtc cttatggtta
      361 tatcggtctg ggtgatattt ccgtactggt tttctttggc tggttgagcg tcatggggag
      421 ctggtattta caggctcata cattgattcc ggcactgatc cttccggcga ccgcctgtgg
      481 cctgctggca acggcagtac tgaacattaa taacctgcgt gatatcaata gcgaccgcga
      541 aaatggcaaa aacacgctgg tggtgcgctt aggtgaagtg aacgcgcgtc gttatcatgc
      601 ctgcctgctg atgggctctc tggtatgtct ggcgctgttt aatctctttt cgctgcatag
      661 cctgtggggc tggctgttcc tgctggcggc accattactg gtgaagcaag cccgttatgt
      721 gatgcgggaa atggacccgg tggcgatgcg accaatgctg gaacgtactg tcaagggagc
      781 gttactgact aacctgctgt ttgttttagg gatattccta agccagtggg cagcataact
      841 gacaaatatc aattaacaat tgatgatttt gccaacagcc cacatagcgc gatatactga
      901 aaattctcgc agcaactgaa tgttaagcct atgaaatacg atacttccga gctttgtgac
      961 atctatcaag aagatgttaa cgtcgtggaa ccgctgttct ccaactttgg cggacgggcg
     1021 tcgtttggcg gacaaataat cacggtaaaa tgtttcgagg acaacgggtt gctgtacgat
     1081 ctgctcgaac agaatggccg tggtcgtgtt cttgtcgtcg atggcggtgg ttctgttcgt
     1141 cgcgcactgg tcgatgctga actggcgcgt ctggcagtac aaaatgaatg ggaaggtctg
     1201 gttatttacg gcgcggtgcg tcaggtagat gacctggaag agttggatat cggcatccag
     1261 gcgatggcgg caattccggt tggtgccgct ggcgaaggca ttggcgaaag cgatgtccgc
     1321 gtcaattttg gcggtgtcac cttcttctcc ggcgaccatc tttatgccga caataccggg
     1381 attattcttt cagaagatcc gctggatatt gaatgataag aaaggcaccg caaggtgcct
     1441 tttttctgcg ttacctgttg gcctacacag taaagaaatt acgcgaaaga tgaagcgtaa
     1501 tcagacctct tccatgcgac ccagcagggc ctgcagacgt tcctgccagc cgttctgctg
     1561 ttctttcaga tggttgttct cacgctccag ctcttcgcgc tgatgctggg cattttgaac
     1621 ttcctgcgac agtgagttgt ttttttcttt cagctcttcg atttccatct gcaacagagt
     1681 gatggtatca atcgcctgct gtacttttgc ttccagtttc tcaaacactt ctaatgacat
     1741 tgtcatacct ctcctgaatt gcaaggcgtt gatggataaa aaatcctcgt cccgattacc
     1801 ggtgacgcct taataaatac gagcgcactt tagttagctc cgattgtatg aagccgcgcc
     1861 atcgctgtcc agcggcacgc cttgcagatt acggtttgcc acacttttca tccttctcct
     1921 ggagacataa tccacaccaa tcgaaaatgt taataaattt gttgcgcgaa tgatctaaca
     1981 aacatgcatc atgtacaatc agatggaata aatggcgcga taacgctcat tttatgacga
     2041 agcacacaca ttttaagttc gatatttctc gtttttgctc gttaacgata agtttacagc
     2101 atgcctacaa gcatcgtgga ggtccgtgac tttcacgcat acaacaaaca ttaactcttc
     2161 aggatccgat tatgagtcaa acatcaacct tgaaaggcca gtgcattgct gaattcctcg
     2221 gtaccgggtt gttgattttt ttcggtgtgg gttgcgttgc agcactaaaa gtcgctggtg
     2281 cgtcttttgg tcagtgggaa atcagtgtca tttggggact gggggtggca atggctatct
     2341 acctgaccgc aggggtttcc ggcgcgcatc ttaatcccgc tgttaccatt gcattgtggc
     2401 tgtttgcctg tttcgacaag cgcaaagtta ttccttttat cgtttcacaa gttgccggcg
     2461 ctttctgcgc tgcggcttta gtttacgggc tttactacaa tttatttttc gacttcgagc
     2521 agactcatca cattgttcgc ggcagcgttg aaagtgttga tctggctggc actttctcta
     2581 cttaccctaa tcctcatatc aattttgtgc aggctttcgc agttgagatg gtgattaccg
     2641 ctattctgat ggggctgatc ctggcgttaa cggacgatgg caacggtgta ccacgcggcc
     2701 ctttggctcc cttgctgatt ggtctactga ttgcggtcat tggcgcatct atgggcccat
     2761 tgacgggttt tgccatgaac ccagcgcgtg actttggtcc gaaagtcttt gcctggctgg
     2821 cgggctgggg caatgtcgcc tttaccggcg gcagagacat tccttacttc ctggtgccgc
     2881 ttttcggccc tatcgttggc gcgattgtag gtgcatttgc ctaccgcaaa ctgattggtc
     2941 gccatttgcc ttgcgatatc tgtgttgtgg aagaaaagga aaccacaact ccttcagaac
     3001 aaaaagcttc gctgtaatgt gactacggga caactaaaca tgactgaaaa aaaatatatc
     3061 gtcgcgctcg accagggcac caccagctcc cgcgcggtcg tgatggatca cgatgccaat
     3121 atcattagcg tgtcgcagcg cgaatttgag caaatctacc caaaaccggg ctgggtagaa
     3181 cacgacccaa tggaaatctg ggccacccaa agctccacgc tggtagaagt gctggcgaaa
     3241 gccgatatca gttccgatca aattgcagct atcggtatca ctaaccagcg tgaaaccact
     3301 attgtctggg aaaaagaaac cggcaagccc atctataacg ccattgtctg gcagtgccgt
     3361 cgtaccgcag aaatctgcga gcatttaaaa cgtgacggtt tagaagatta tatccgtagc
     3421 aataccggtc tggtgattga cccgtacttc tctggcacca aggtgaagtg gatcctcgat
     3481 catgtggaag gctctcgcga gcgtgcgcgt cgtggtgaat tgctgtttgg tacggttgat
     3541 acgtggctta tctggaaaat gactcagggc cgtgtccatg tgaccgatta caccaacgcc
     3601 tctcgtacta tgttgttcaa catccatgcc ctggactggg acgacaaaat gctggaagtg
     3661 ctggatattc cgcgcgagat gctgccagaa gtgcgtcgtt cttccgaagt gtacggtcag
     3721 actaacattg gcggcaaagg cggcacgcgt attccaatct ctgggatcgc cggtgaccag
     3781 caggccgcgc tgtttggtca gttgtgcgtg aaagaaggga tggcgaagaa cacctatggc
     3841 actggctgct ttatgctgat gaacactggc gagaaagcgg tgaaatcaga aaacggcctg
     3901 ctgaccacca tcgcctgcgg cccgactggc gaagtgaact atgcgctgga aggtgcggtg
     3961 tttatggcgg gcgcatccat tcagtggctg cgcgatgaga tgaagttgat taacgacgcg
     4021 tacgattccg aatactttgc caccaaagtg caaaacacca acggcgtata tgtggtccca
     4081 gcatttaccg ggctgggtgc gccgtactgg gacccgtatg cgcgcggggc gattttcggt
     4141 ctgactcgtg gggtaaacgc gaaccacatt atccgcgcga cgctggagtc tattgcttat
     4201 cagacgcgtg acgtgctgga agcaatgcag gccgactctg gtattcgtct gcacgccctg
     4261 cgcgtggatg gcggcgcagt agcaaacaat ttcctgatgc agttccagtc cgatattctc
     4321 ggtacgcgcg ttgagcgccc ggaagtgcgc gaagtcaccg cattgggtgc ggcctatctt
     4381 gctggtctgg cggttggctt ctggcagaac ctcgacgagc tgcaagagaa agcggtgatt
     4441 gagcgcgagt tccgtccagg catcgaaacc actgagcgta attaccgtta cgcaggctgg
     4501 aaaaaagcgg tcaaacgcgc gatggcgtgg gaagaacacg acgagtaatg tatgccggat
     4561 gaagcgtttt tgccgcatcc ggtagtcccg aaacgtgcgg gggcaacccc gcacacatca
     4621 ataatccctc ccttcccctg tgctacactt cgcgccattc cttactgctt agagtttgct
     4681 atgagacgag aacttgccat cgaattttcc cgcgtcaccg aatcagcggc gctggctggc
     4741 tacaaatggt taggacgcgg cgataaaaac accgcggacg gcgcggcggt aaacgccatg
     4801 cgtattatgc tcaaccaggt caacattgac ggcaccatcg tcattggtga aggtgaaatc
     4861 gacgaagcac cgatgctcta cattggtgaa aaagtcggta ctggtcgcgg cgacgcggta
     4921 gatattgctg ttgatccgat tgaaggcacg cgcatgacgg cgatgggcca ggctaacgcg
     4981 ctggcggtgc tggcagtagg cgataaaggc tgcttcctca atgcgccgga tatgtatatg
     5041 gagaagctga ttgtcgggcc gggagccaaa ggcaccattg atctgaacct gccgctggcg
     5101 gataacctgc gcaatgtagc ggcggcgctc ggcaaaccgt tgagcgaact gacggtaacg
     5161 attctggcta aaccacgcca cgatgccgtt atcgctgaaa tgcagcaact cggcgtacgc
     5221 gtatttgcta ttccggacgg cgacgttgcg gcctcaattc tcacctgtat gccagacagc
     5281 gaagttgacg tgctgtacgg tattggtggc gcgccggaag gcgtagtttc tgcggcggtg
     5341 atccgcgcat tagatggcga catgaacggt cgtctgctgg cgcgtcatga cgtcaaaggc
     5401 gacaacgaag agaatcgtcg cattggcgag caggagctgg cacgctgcaa agcgatgggc
     5461 atcgaagccg gtaaagtatt gcgcctgggc gatatggcgc gcagcgataa cgtcatcttc
     5521 tctgccaccg gtattaccaa aggcgatctg ctggaaggca ttagccgcaa aggcaatatc
     5581 gcgactaccg aaacgctgct gatccgcggc aagtcacgca ccattcgccg cattcagtcc
     5641 atccactatc tggatcgcaa agacccggaa atgcaggtgc acatcctctg attgatttga
     5701 tcgattgagc cttccagtcc ttcgggactg gaattttttt gttcggagaa cgaagataag
     5761 gcaagttaat caaaacagga gaaaaacatg gctgattggg taacaggcaa agtcactaaa
     5821 gtgcagaact ggaccgacgc cctgtttagt ctcaccgttc acgcccccgt gcttccgttt
     5881 accgccgggc aattcaccaa gcttggcctt gaaatcgacg gcgaacgcgt ccagcgcgcc
     5941 tactcctatg ttaactcgcc cgataatccc gatctggagt tttacctggt caccgtcccc
     6001 gatggcaaat taagcccacg attggcggca ctgaaaccag gcgatgaagt gcaggtggtt
     6061 agcgaagcag ctggcttctt tgttctggat gaagtaccgg actgcgaaac gctatggatg
     6121 ctggcaaccg gtacagcgat tggcccttat ttatcgattc tgcaactagg caaagattta
     6181 gatcgcttca aaaatctggt cctggttcac gccgcacgtt atgccgccga cttaagctat
     6241 ttgccactga tgcaagaact ggaaaaacgc tacgaaggga aactgcgcat tcagacggtg
     6301 gtcagtcggg aaacggcagc ggggtcgctc accgggcgga taccggcgtt aattgaaagt
     6361 ggggaactgg aaagcgcgat tggcctgccg atgaataaag aaaccagcca tgtgatgctg
     6421 tgcggcaatc cacagatggt gcgcgataca caacagttgc tgaaagagac ccggcagatg
     6481 acgaaacatt tacgtcgccg accgggccat atgacagcgg agcattactg gtaagcggtt
     6541 acttatcgat aaacggcacg atgagcaaat ccgcactcat cttattgatc atcccgcgat
     6601 atgccggcat caaacggttg ataaatgagt gatgatgacc acagacaagg aggtcgcact
     6661 gctctttttg cataatttcc agcagtgttt ccggcatttc tccgcgttca atacgcagtt
     6721 ttgtcttcgg ccattgaata tttttcgtca gtttatacag cttgttatcc gacttattct
     6781 tcaacaattg aagaatatct tctgttgcag ggaagtagat acccggatac aactcgctta
     6841 agccatcatc aatatgaatt aacgtcaggt gagcgtcatt atgtctggcg agctccagag
     6901 ctttattcac cagtaaggca tcttcttcat tcccggaaat tgccacgcca atgtgtttat
     6961 aagccatgtt taactccttc taaagcaact ccatcaagct aacgaacgca gtgataactc
     7021 aaaaataatc atctcggcct ggcatgagaa agtgaaggcc gcatcaagct caacttgccc
     7081 ctccagatcc ttaatcttgt aggccacgct ggccccttca tctgcagagg atgcagcggc
     7141 ggcaagctct ttcagtttgg tcataaaaga ctctgcttct tcgcgggtgg caaaaaagcg
     7201 cgaaaactta ctggtacagt tgtcgttatc aatcaccgta ccgatatcaa tcgcacatcc
     7261 tttagtactg catttatcta cgacatcttt catagggcac ctctgtatat tcgcctctct
     7321 gtcgattacg acttaagaat aatgcgccgt taagggaaga acaaacaagt aatgatctta
     7381 tggattacca ttttttttga tattgcttaa ggtgaagagt tcagaaggat tttccgggta
     7441 ttgtcatttg catcgtggaa ataacaatcc aggaaaaaaa tagcagtaag agatatatta
     7501 aaggttaatt acttgattta ttgtcggctt tatacttcac atcctgagta tctttaccat
     7561 atttattttc gccttgtgtg ccaacaaacg caccaagatc gataagcatc atcaccagaa
     7621 tcaacgtcgg gacaaaacgc cccaccgccc attgccagac acccggtaaa atcgcccagt
     7681 tagccgccag cagcatccac gccacaatca tcagaaatgc ccatgcgccg gaacgcccgc
     7741 gatcatgcaa gcgcttaaca gttactgccg ctgttggcca aagcaagcac acaaggcaaa
     7801 acgccgcggt ctgaatatcg agtaaatttt tacccgccag tgagaaaagc accagcatgc
     7861 ctgcgaacca caggcctatc caaatccaga aatcacggcg tccaatacgc cctttaaatg
     7921 agaataacca ttgctgtatg gtcatgtaag ttccttgatg gttgtctttt ccaggattct
     7981 acccttttga caagggcgac agatatcgtt ttaatcggag ccagtcataa caaaaggtac
     8041 tgtcaatgaa gccagggtgt acgctgtttt ttctcttatg ttctgcatta accgttacaa
     8101 caacggcgca tgcacaaaca ccagatacgg caacgaccgc gccttatctg ctggctggag
     8161 cccctacttt cgatctctcc atcagccagt ttcgagaaga ctttaacagc cagaatccta
     8221 gcctgccact gaacgaattt cgtgccatcg acagcagtcc cgacaaagcc aatctcactc
     8281 gtgctgcaag taaaattaac gagaacttgt atgcttctac agcgctggag cgcggtacct
     8341 taaaaatcaa aagcattcaa atgacctggc tacccatcca ggggccagag caaaaagcag
     8401 cgaaagcgaa agctcaggaa tacatggcag cagtgatccg cacactcacc ccattaatga
     8461 ccaaaacaca aagccagaaa aaactgcagt cgctgctaac ggcggggaaa aacaaacgtt
     8521 attacaccga gacagaaggt gcactgcgtt atgttgtcgc ggacaacggc gaaaaggggc
     8581 tgaccttcgc tgttgaaccg attaagctgg cgctatctga atcgcttgaa ggtttgaata
     8641 aatgacaaaa agcaaagcct ttgtgccgat gaatctctat actgtttcac agacctgctg
     8701 ccctgcgggg cggccatctt cctttattcg cttataagcg tggagaatta aaatgcgaca
     8761 tcctttagtg atgggtaact ggaaactgaa cggcagccgc cacatggttc acgagctggt
     8821 ttctaacctg cgtaaagagc tggcaggtgt tgctggctgt gcggttgcaa tcgcaccacc
     8881 ggaaatgtac atcgatatgg cgaagcgcga agctgaaggc agccacatca tgctgggtgc
     8941 gcaaaacgtg gacctgaacc tgtccggcgc attcaccggt gaaacttctg ccgctatgct
     9001 gaaagacatc ggcgctcagt acatcatcat cggccactct gaacgtcgta cttaccacaa
     9061 agagtctgac gaactgatcg cgaaaaaatt cgcggtgctg aaagagcagg gcctgactcc
     9121 ggttctgtgc atcggtgaaa ccgaagctga aaacgaagcg ggcaaaactg aagaagtttg
     9181 cgcacgtcag atcgacgcgg tactgaaaac tcagggtgct gcggcattcg aaggtgcggt
     9241 tatcgcttac gaacctgtat gggcaatcgg tactggcaaa tctgcaactc cggctcaggc
     9301 acaggctgtt cacaaattca tccgtgacca catcgctaaa gttgacgcta acatcgctga
     9361 acaagtgatc attcagtacg gcggctctgt aaacgcgtct aacgctgcag aactgtttgc
     9421 tcagccagat atcgacggcg cgctggttgg cggtgcttct ctgaaagctg acgctttcgc
     9481 agtgatcgtt aaagctgcag aagcggctaa acaggcttaa gtctgacagg tgccggattt
     9541 catatccggc acttactttc cttaactctt cgccttaacg caaaatctca cactgatgat
     9601 cctgaatttc ctcggctgaa gcacggttaa gcgtcagtag atttcgttgt gtcgccagca
     9661 atacaaatga gttatcactc tgccgtacca tcgccagccc gtagcttccc atatgttccc
     9721 gcgcctcagg tacttcttct gccagcatca taaatgggct gcgttgtacc agttcgcttt
     9781 ccgttacccg acgcgccagg tattcatgcc cgcgcaaacc acctggcagt ggcaaccagc
     9841 ggctgctgat gttcgccaga ttgttatcca gttgtttgcg cacatcagga cgaatacaag
     9901 agatatgaat atgaaaatgg ttttgcgtac gcccggtgcg ggagttgatc gccaaagaaa
     9961 ccgcgcgatc gggaaccggc tggccgtatt ttttgctcat aaaatcacgc gcctgccagg
    10021 ccaaccaaaa gaagttcggc gttgaaggat cggtcaacaa aggactttca gtaccgttaa
    10081 tacgatacgt tggcatcaac agatattgca gtgggccatt aagatctttt aaaaccacgt
    10141 atccggcatt gggtttgact tccgcacatg gcgaaggatt ttgattttgc tgctgattgg
    10201 gcaaacattc ctcaaggaca atcttacgta atgtatccga ctcttcaccg gttaatttcc
    10261 agtaaccaat accggcagcc acaacggcga taactatcat caccaaaaaa agaagacccg
    10321 cttttttcat ctttttttcc ctgtacctca aagagatgca agggtaacgc aaaatcgtga
    10381 caaataaaaa acccggtcgc gagaaaccac cgggtttgat aattatcctg gagaaatcag
    10441 cgtttgctga tctgatcgaa cgtaccgccg ttagcaaaat gctctttttg cgctttcgtc
    10501 cagccgccga actcttcatc aatggtgaat aacttcagct ttggaaacgc attttcgtac
    10561 tttttcgcta cttcagcgtc gcgcggacgg tagtagtttt tcgcggcaat ttcctgacct
    10621 tctggcgagt agagatattt caggtaggct tccgccacct ctttggtgcc ttttttctcg
    10681 accactttat cgaccaccga cacggttggc tctgcgagga tagactcact cggcgtgacg
    10741 atttcgaatt tatctttccc cagttcattc gctgccagca gaacttcgtt ttcccaagcg
    10801 atcagtacat cgccaatccc gcgctcgaca aaggtgttag tggagccgcg cgcgccagaa
    10861 tccagaactt cgacgttttt atacagtgcc cgaacaaaat cctgtgcttt tgcctgatcg
    10921 ttgttgttgt gatgcagcgc gtagccccag gcagccaggt agttccagcg tgcgccacca
    10981 gagcttttcg gattaggcgt gatcaccgaa acacccggtt taatcagatc gttccagtca
    11041 tggatctgct tcggattgcc cttacgcacc aggaaaacaa tggtggaagt gtacggtgcg
    11101 gagttatccg gcagacgttt gatccactct ttatcaatcc gcccgcgttc cgcaatcgcg
    11161 tccacgtcat aggccagagc cagcgtgaca acatcagctt caataccgtt gattaccgac
    11221 gtcgcttgtt tacctgaacc accgtgtgac tgacgaatca ccacgttatc accagtttgc
    11281 tgtttccagt gggcgctgaa tgccttgttg tactgttcgt acaattcgcg cgttggatca
    11341 tatgaaacgt taagaagctg aatatccttt gccataacgc tggttgccgc cagcaaaaat
    11401 gttaacccta cgccccactt gttcatcgcc cgactctctt atgttgtgtt gtgatgagca
    11461 aagcgtgcca gaaggttaac caaacattaa agaataaaaa aagattggct ataacttgcg
    11521 ggtatatgtt gagggattaa aaaggcggaa gaaatccgcc tcatattgct gacaaagtgc
    11581 gctttgtcca tgccggatgc ggcgcgaacg ccttatccgg cctacaaaag tttgcaaatt
    11641 caataaattg cagaattcat gtaggcctga taagcgaagc gcatcaggca tttttgcttc
    11701 tgtcatcggt ttcaggctaa aggaatctgc ctttttccga aatcattaat acagtttttt
    11761 cgcgcagtcc agccagtcac ctttgaacgg acgcttcatg ttttcgatag cgtcgatgat
    11821 gtcgtggtga accagctgtt cgttctggat acctacacaa cgaccgccgt aacctgccag
    11881 cagcagatcg atagcgtaag cgcccatacg ggaagccaga atacggtcgt aaggcaccgg
    11941 agaaccaccg cgctggatgt ggcccagcac agttgcgcgg gtttcacgac cggtttcttt
    12001 ctcgatgaaa tgcgccagtt cgtcaacatc acacatatgt tcggtaatcg ccacgatcgc
    12061 gtgtttttta cctttcgcga tacccgcttt gatttcgttt accaggtctt cacggctgaa
    12121 ttcaacttcc ggaaccacaa cgaattcaca gccaccggca atggccgcag ccagggtcag
    12181 atcgccacaa taacggccca tcacttccac cacggaaata cgctggtgag aagaagaggt
    12241 gtcacgcaga cggtcgatcg cttctacaac ggtgctcagc gcagtgaaga aaccgatagt
    12301 gtagtcagtg cctttgatgt cgttgtcgat agtgcccggc agaccgatgc acgggaagcc
    12361 catttcggtc agacgcattg cacccatgta ggaaccgtca ccgccgataa ccaccagcgc
    12421 gtcgatccca cgttttttca ggttttcgat agccacggcg cggatgttct cgtcgcggaa
    12481 ttccgggaaa cgcgcagaac cgaggaacgt accgccacgg ttgatcatgt cagacacgct
    12541 gtaacggtct agctgtacca tacggtcttc atacagaccc agatagccgt cataaatacc
    12601 cattacttcc agaccttctg tcagcgcaga acgaacaacc ccgcgaattg cggcgttcat
    12661 gcctggcgca tcaccgccgc ttgtcaacac accgattttc ttaatcatga ctacctctga
    12721 actttggaat gcaaaataaa atctgttgcc ggaagtcttc ttgcacatcg aagtgatcca
    12781 acgaatgtgc aaatagtata acaatcactt cctgctgaat tgattcaggt caggccaaat
    12841 ggcggtattt tatacacaaa atgcgggtct ggctctcttt tatactgatt atgaaagcat
    12901 agaccgttta ccctccctgg gtacgacgga acaggggtcc tgatggataa tcacatccga
    12961 tcccggaaaa cgccgtaaaa tagcctgctc tacctgatcc gccaccatat gtgcctgaac
    13021 caaaggcaga gagtcttcca tttccaaatg aatctgaata aagcgggtcg gccctgactg
    13081 ccgcgtgcga agatcgtgag cgccgctaac acccggccag gaagtcacga tatcaataat
    13141 ttcttgccgt tcctcatcag gcaatgcgcg atccagtaat gactgtaccg cctcatatcc
    13201 catgcgtaac gcgctatata aaatatagat gccgattccc aatgcaaaca gagcatcggc
    13261 gcgatgccag ccgtaccagg acaaccccag cgccagcaga attgcgccgt tcatcataac
    13321 atcagactgg taatgtagca tatcagccct caccgcctgg ctttgcgtcc ggcgcaccac
    13381 ccaacgctga aacgagacaa ggataatcgt acaaattagc gccacaattg tcacgataac
    13441 cccgacgcct ggatctgtca tcggtgttgg agataccaga tgttgaatac ccgtcaaaaa
    13501 caggaatagt gccgaaccgg agataaacat actttgcgcc agcgccgcga gggactcagc
    13561 tttaccgtga ccaaacgagt gattatcgtc ggcaggttgc agggaatatc gcaccaccag
    13621 taaattcgtc aacgacgcgc cgatatccac cagcgaatcc accagcgcgg cgagaatact
    13681 caccgacccg gtataccacc atgcaaaaat tttaatcagc aatagcagcg aagccatcgc
    13741 cgtcgcagca atcgccgccc gactgaccag ccgtccataa gattgattca taaatactcc
    13801 cgctatcaac tgacgctagt ataacggaag caaatcatct gcaatgcatt aagcagcagg
    13861 caaattgagg ataaaaaaaa cccccacatc atgtggggga agacagggat ggtgtcacaa
    13921 aaaagcacct aacctgatga tataaatgga tttatatcaa acactgtcca catagcgtcc
    13981 acatcgacta ctgaaagccc ctaagcacat ggggctttat ttttcctctt tttggaggta
    14041 tgaattttac tcaaccaaat gtttattaag gattttctat ggcaactctt aaatcagtga
    14101 tggcaatagc caccttaata atttcactca acgcaaacgc gaatgatatt aaaccccttc
    14161 aaaagcagct taaaccatgg caacccctag aaatcacgaa taaaaacaac tcgcttacgg
    14221 tggtgctccc tggcaaagaa atctctcctg acgcctacaa caacctgatt atgactggtg
    14281 tatgcacacc agtctggaca aaagacacgc ctgtttcata tctcaaaaaa atcaaagaaa
    14341 taagcatagt taatcaatat aaatccatcg gttatgtttt ttccgaacca ttaaaaacat
    14401 gcaatgaaat aggttcgttg acgcctaaac aggggcaagt gttactttca tcccaaacac
    14461 gtctttacac agggaaataa gctcctaaac aaagttgaaa gcccctcgac tgaggggctt
    14521 tctgtttgta attacatcca cataatttgc tgccctgaag gcaatgggtg cggcctcacg
    14581 gcgtggactt ctcccggctt cacgatgtat cgctgtaccg actcataagt gatgaacgtg
    14641 gcgctgcaat tcacgttctg acactggtga taacgctctt ttgtcgtgtc agtgatatag
    14701 cgacttgtac gcgcatgtgc ggcatgctgg cataaaggac aatgaaacat cgcgagcacc
    14761 tcttccggtt ttgttgatgg tgccatttta gttaatttat ccttataaaa caaatagata
    14821 aaataaaaac atcactcatc atcttctgtt tcgtactcca catcagaaag cctgacctca
    14881 agctctaagg acgtcgtgaa gccgctatta ttcagaaaat gtgtcacctt agtgattgtc
    14941 cagtcctgct cgtctatgac gcgcttaaag cctgacactt taaccggtgt ttccgtgtaa
    15001 atatctgccc gaccggtagc caggctgatg gagaactccg ctacgccccg ttgcagttta
    15061 tcccacttcg cctgagcggc gcgcatggcc tgcgctttcg tggcatatac cgtggtcagg
    15121 gcaaaaacat tgtcagcctc accggccatg tattcacctt cgcgcgcttc cggtactttt
    15181 ggcgctttct tctgcgtgac tggtttcgct ttcgggtgct ccagtgcgcg caggtgtttc
    15241 tctttctttt tgcgtttcag ttttaccttc tgcttttgcg gcttcgggtc tttggtgtgt
    15301 aaccactttg ccgttacgcc ggtgtaagct ccacggtcag caatcgcaaa atgatgacgg
    15361 tcaccgtcgc tgcgggtgat ggtaatctgc gggatttttt taccgcttgc cgtcaccccc
    15421 tgccccgctt tgagaaacag cagttttccc atttttaccg acacctcacc gccgttgcgt
    15481 tcagcaagac gggtcaggaa tttcgcatca gactcctgcg actggtcgat gtgcgggatt
    15541 ttaattccgg ccagtgacgg agcgacactg gcttccagcc tgttacggga ggctatcgcc
    15601 tcaacaatcg caccgagcgt ggtgtcatgc catgaacctt cacggcggga attgagcgtc
    15661 ccgcgaaaat ctgcactccg ggcgcggatg gtgaccacat ccggtgcgcc ccggtgttca
    15721 acctcatcaa cggtaaattt ccctttgcat accatggcaa aacctttcca gccgatatac
    15781 accgtcagga cagcgccacg aaccggcagc ccgacctgcc cgtcggcatc gttcagttca
    15841 atatcaagct ggtcagcctc aaagccccgg ttatccgtca gggtcatgct catcagacgg
    15901 tcgctgatat tgccggtaat atccctgctg tcgagcatca gcatataatc cggcgtcagc
    15961 gtactgcctg catcaaatgt catcgcatcc agcattatcc cgcccccgtc atacccgtga
    16021 atttagtcgc catactgcca gccttaccga tgaacgattc cgcctgttta ccgatatcgc
    16081 cataaagcgc ggccagtgat tcatcaacgc gggtgagcga cagcgtaaaa tcaatttttc
    16141 ggggtgtgcc gtctgcaaag aaaatactcc ctgtttcact caccttgctg atgacataca
    16201 tgccgtaaat catgccggtg ccatccagca acggccacgc ccgcccctcc tctgccatca
    16261 gcctgagcgt ggtcatcgtc agctttccgc cggtcagttc gggataaagc acaccggcaa
    16321 gcgtgatgtt ttcttcaccc acaccgagaa actggtaggc atcccgttta ccgatacggg
    16381 aatttgacgg ccagcgataa tctgattcac gctgcatggt ctggtgtggc agcgtctggc
    16441 gcataaaaac aaacatacct aacgcgagca tcatttttcg tcacctcctt aaccgtcatg
    16501 catcatgctg gcacgggcgc gcgcacgttt atcccgctcg tatttttcga gcgcatcctg
    16561 taactggcgg tcaagctgtg tccccggcgc agtaccactc gtaaggctga tgtgatattc
    16621 gtttttactc tggtctacat aagagcggcc agccggtgcc gtgaccggct gataagcctg
    16681 atagcctgca taagagctgg tcgccggaat atacccaccg gtgccatacg tggcggcatg
    16741 agtccttgcg gcggtctggt caagtgtgcc tgactctttg ttgataacac cgagtttttc
    16801 cagtacccag tcaataccgc tgcgcagttt gttgaacgca ttaagcggca gcatcagcgc
    16861 gtcagccagt gcctgcccga acatgacgcc cgtgtcacgg caacggttca gggtgtcctg
    16921 ggtggctttg accggggcaa tcaggttttt aaaccactgc cacgcggcct gtaacttttc
    16981 acccagccag tcaaacaccg gcttaagtgg cgtgaacagt tcccccaccg gcgcaaatgc
    17041 cgctttcagc ccttccacca caccgccaaa gaatgcgctg acaggctccc agtatttacg
    17101 gataagtaac gctccggcga caatggcggc caccacggcc actaccggcc agctaatcgc
    17161 cccgatggcc gtcataacgg cactgccaac cgtcgtgaaa attgccccca ttgcgcctgc
    17221 tgcagcgatg atggcattga tgccggtgat aaccggccag gctacaagac caatggcacc
    17281 gatgatgcca gtaagcgcca gtgcgccacc ggcaatgagg ccgatggttg acgccagtga
    17341 tttgtttttc tggatccagc catcgagttt taacacatac tttgtggccg tctgcgtgag
    17401 attacgcagt gcgtcttcct gctggtcaaa caggtcagtc cccaccgcct cataagcgga
    17461 ctgaaactct ttaaagtcac cgccgaggtt gtcctgcatg atatttacca gctcggcggt
    17521 cttcccgtct gaggctttaa acgcagcggt cagtttgtcc agctttccgg ttgaggcggc
    17581 agtcatcagc acggcggcgg ctgagctggc ctcctccccg aaaatggttt tcatgtattc
    17641 agcctgctgg gcagtaccga gccggttttt ctcaaaactg gcctgcattt ctttcagaat
    17701 ggtaaatatt ggtcgggtgt ttcccttgct gtctgaggtt ttcacaccaa gctctttcag
    17761 tgcatcccat gcttttcccg tcggtgcctg caggcggctt aacacggcac ggcttcccgt
    17821 ccccgccatt gaaccggtaa tttttgcatc atgcagcgcc ccgaccattg cggcggtttc
    17881 ttcaatgctg acaccggcat tttttgccac aggtgcggca taggtcagcg catcgctcat
    17941 gccgtcaaaa tcggcggcgg ttttgttcat cgtcatggag agaacatccc cgatatgagc
    18001 gaccttatcg tttgaaagct gaaaggcgga tttcatcccc atcaacaggg cggcgttttc
    18061 ttccatcgtg cgacggttcg ccagcgccat attcagcgtg accggcgttg ccgcctgaat
    18121 ggcatcaaca tccccacccg ctttcgcgat gattatctgt gcaccggctg catcatccgc
    18181 cgaggctgcg gtattgtcgc cgagctggcg cgcctgcttg cggagcgcgg ccatttcggc
    18241 agagtctttt gccactccga gcacagcctg taattctgag tttttctgcg caaactcata
    18301 accgggcatc agcagcttaa caccggccat cgttcccgcc gccgcaatcc ccacaccggc
    18361 agcgcccact gaggccatat ttccggccag ctcctttccg gcctgataac gctgttttac
    18421 tgcgttaagt tttgcctgtt gcacactgac acgcgccagc gcatcacgct gacggttaag
    18481 ctgtgtggtg gtttcactga tacggttttt cagccctcgt tcatcatgcg ccagattacg
    18541 ggtattaata cccgcctgac tcaattcctg acgctggcgc tgtaccgaca atctcaggct
    18601 attatatttc gcctgtaact cagacgcatt tttacgcgcg gcttccattg cctttgcctg
    18661 tgcatttgtc ggtcgttcag tatttttaaa ctggacagcc agagctgcgg cttcctgtct
    18721 ggcttttttc agttcctgac cagtcacggc gagctgtgca ctggtcttgc gaaatccctc
    18781 aatacgggat gcctgaccgt tcagctcgcg cagtgatttt tgtgtttccc ggatatcccc
    18841 cgacagcgac ttgctcgctg tacggatgga tttaaacggg cgggatgcct ggtcaacagc
    18901 cctgagcaat acctgtaatt ttacattgtt actcattcgt gtttccgctt cgccggagcg
    18961 ccttttcgcg ccatgtgatg agttcggtca ggctcatggg atacagttct gatggcggcc
    19021 agtgaaatat cactgccaca tccgccatca ggtcatcgac cgagagattt ttcggaaacg
    19081 ttactgcacc gagttcggcg acaaaaaacc gaccacctta ccggccagcg ccacaaggtc
    19141 aggcagttcc agcgcggcaa cttcctgttc ggtcagcatc ggtgccgtca tgcgcggcag
    19201 cactttaatc agtgcatcga cttcggagtt cgcgaccgca gccagactga caccgcgcag
    19261 cgtcccggca ctgggtttca tcagcgtgat ctgttcgatg acctgctcac cacgcttgac
    19321 cggattgtcc agggtaatca cattttcttt gttcatggtt ttctcacttc tgaatcaggg
    19381 ttaaccggtc agcctggctg accggatgaa aatcacaggc cgatattgcg gcggtgttgc
    19441 tccagccggt cgacgccgtt caccttctca atcatgttga tggtgtcgat ttcgaccagc
    19501 tccttaccgt ccatcgtcag ccggaaatag gtgcagacca cggagatttt cgactcggtg
    19561 tcttctccct gtttaccctc gccggtgtcg atttctttct gacggccacg catgaccact
    19621 tcgacggcca ccgtttcgcc ggtatcgtcg cgctggtaag agccagcaaa acgaatcggt
    19681 acggcatcca caccggttgc ggcgtaaagc tcccagataa ccgaatccgg gaagcccccg
    19741 agcgaccact ccattgacag cgcatcgtca tcaaggccga ggtctaccgg tgcgctgccg
    19801 ttcatccccg caccgcgata gttttcgagc ttacgggtca gttttggcag cgtgacggac
    19861 tttgcaacgc cctgatagct gtagccgttc agaaagacgt tcattaactt gagtttgcgc
    19921 ggcattgcca tcggtcaggc tccttaattg ctgttaaccg aggtaaccag acttgccagg
    19981 tatctggaag taatacgctg gcgcagggtc aggttttcga gaggaggcac cggggtatag
    20041 tcgtagtcga gatacagttt tccggccttg agggtttccg catcattggc ttcttcactg
    20101 aaccagcatg tgccatccac gatataaccg ttgtttttca gttcgcggaa tttggcatta
    20161 atgccatcaa tgatgtcacg aatcagcgtt gcggtaattg gcttatcaat agcccacatg
    20221 tgcgcctcgg ccatcgtgtc ggccagtacc tgcgcggtgc gggtgtagct ttcaaagagg
    20281 aacagcgggt catcagagca ggtacggtta atcaatgttc attgactcgt agttctccac
    20341 gcggtcgagt ttcgggtttt cctcaatcac gcggcgatgg ctgtcatcca tgtagtagat
    20401 ggacaggttt tccagcttcg tgatgagcat cgcatccgcc gggaagtacg ggacgcgtac
    20461 cgccggcagg ttaccgatgc gtttctggct gatgatgacg tcagcggcca gcatttcgct
    20521 gttgtcctgc tccttgttga cgatggggaa atacttgtcc gccagtaact gacgccccac
    20581 aatcaccaca aggtcagggt cttcctgata ccacggctca atcaggttgt tggtcgcatc
    20641 catcaccagt gcatcgaggc tggcataatc accgccctta cccacgcgga tgacctcaga
    20701 ggtggtgtga ccttcctcgt cagtaacctt gctcatcacg cgcgccgggg cttcattgcg
    20761 gtatttctgc agccagccga ccgccacatc ctgcagcatc gggttactgc tgcggtcaga
    20821 ggtttcggca cgcttcacgc cgttaaaacc ggccatgatg aaatcaaggg actggcgttt
    20881 gataatggcg ttacggacac ggagctggaa atcctgataa cgcgcccaca ggtccagcgt
    20941 tttgtagcgg atataaaaat cgaagttaat ctggtcgcat tcgtacttgt ttgacgccag
    21001 cttcgagaag tccttcggct gacgctcggt gccaccggcg gtgtcggtgg tgctggcgat
    21061 ggagccggtg acaccgatgc caattttttc ccctttcatt tcgctgaccg gcacaatgtt
    21121 gatgcgggtc agaaagtcag aggactcctg catggtgttc atcagggtct gggtgaccga
    21181 cggttcaacg gtgaattttt tcgacacatc accggcgtcg atgccgttca gttcggcaac
    21241 acgggacagg taagcattaa atttaaagcg ggtttcctgg cgcatagttt ttcctgaaat
    21301 taagggttaa tcgtgaaggt tttcccggac tgactgacgc cggtcagcag ttcgtcatca
    21361 gggcgtcacc gccaccaccg gtggccttgc tgcggcgctg ctgggtcaga ctttcggtgt
    21421 ggtcgagact gtttttcagg cgggtgaatg cctggctggt ttcatccgcc ctgtcagtca
    21481 cctcctgctt aagtgcggaa aaggcggttt ctatctcagc gaggcgctgc tcagtggcgc
    21541 tcagtttttc ctgcacatgt tcagcaacag cggtcaccgc ttcatgcaca tcattcagac
    21601 gggcgtcatc gctggcctgt ttgcggccaa aaatggattt caccttttcg gtcagggcgg
    21661 tgaacacggt ttcaggcagg tcttcaaatt ccagctcaac gggcgttgcc actgaaatca
    21721 ggttttcagg gcttaatttg aagcggttca gagggttgtg ttttgccgtg cggcagaatt
    21781 ccaggtattc cgtgccgagg cttgccgggt catcggtgac ggccagcccc accagataac
    21841 atttgccggt gttggcaaag ttcggctgaa tttccattga ggtgtagacc ttctgcgcgg
    21901 ccttgttcat cgcgataagg tcatcggtcg gggtgatttt cgcaaacagc gcccatttgc
    21961 ctttcagcgc cgaatcgtca tcaatctttt cggccttcag ttcggccaca tcgccataac
    22021 gtttaaaaat accgtcaggc aggatgccgc gcagatgttc caggttaatg cggcaaccgt
    22081 agacacgcgg gtcaaaggtt tcggccattt cctgaatatc ctgcgcactg atgacacgcc
    22141 cgtcacaggt gtcaccctca acgccgatac gaaagaattt tgagactttt tttgccattg
    22201 tcaggagtcc tgaatagtga ttagaggagt cacatgtcgg catcagtttc ccgacgatgc
    22261 gcatcctccg ccatcagtcc cggatggctt atcactgaca caacagcacc ttagcgaatc
    22321 gcggggcgcg actcagtagc cttgccgtgt attcatcacg gcgaggtatt catgaccatc
    22381 accacagaca ccactctttt acacgacccg cgtcgtcagg cggcgctgct gtactggcag
    22441 gggttttccg tgccgcagat tgccgccatg ttgcagatga aacgcccgac ggtgcagagc
    22501 tggaaacagc gcgacggctg ggacagcgtt gcccccatca gccgtgtcga aatgagcctg
    22561 gaagcgcggc tgacccagct catcatcaaa ccgcagaaaa ccggcggtga cttcaaggaa
    22621 attgacctgc tgggacgcca gattgaacga ctggcgcggg tcaaccgcta cagtcagacc
    22681 ggcaacgagg cagaccttaa tccgaacgtc gctaaccgca acaaaggcgg gcgtcgcaaa
    22741 ccgaaaaaga attttttcag tgacgaagcc atcgaaaagc tggagcagat tttctttgag
    22801 cagtctttcg actatcagtt gcactggtat cgcgccgggc ttgagcaccg catccgcgat
    22861 atactgaaat cccgccagat tggcgcaacg ttttattttt cccgcgaggc gctgctgcgc
    22921 gccctgaaaa ccggccataa ccagattttt ctgtcggcca gtaaaacgca ggcgtatgtg
    22981 ttccgcgaat acatcatcgc ctttgcccgg ctggttgacg ttgacctgac cggtgacccg
    23041 attgtcctgg gcaataacgg cgcaaaactg atttttctcg gcaccaactc caacaccgcg
    23101 cagagccata acggcgacct gtacgtcgac gagattttct ggatcccgaa ttttcaggta
    23161 ctgcgtaagg tggcatcagg tatggcctca cagagtcacc tgcgttcgac ctatttctcc
    23221 accccgtcca cgctggcgca cgacgcctac ccgttctggt caggtgaact gttcaaccgg
    23281 ggacgcgcca gcgccgccga acgcgtggaa atcgacgtca gtcataacgc ccttgccggt
    23341 gggcttctct gtgcggacgg ccagtggcgg cagattgtca ccattgagga cgccctgaaa
    23401 ggcggctgca cgctgttcga cattgagcag ctcaaacgcg aaaacagcgc cgacgatttt
    23461 aaaaacctgt tcatgtgtga atttgttgac gacaaggcat cggtgttccc gttcgaggag
    23521 ttgcaacgct gcatggtcga cacgctggaa gaatgggaag actatgcgcc gtttgccgcc
    23581 aatccgttcg gctcccgccc ggtatggatt ggttacgacc cgtcacaccg tggcgacagc
    23641 gccggatgcg tggtgctggc accgccggtg gtggccggtg gcaaattcag aatacttgag
    23701 cgtcaccagt ggaaaggcat ggactttgcc acccaggcgg aatccatccg caaactcacc
    23761 gaaaaatata acgttgaata catcgggatt gatgccaccg gcctcggtgt cggcgtgttc
    23821 cagctcgtgc gctcgttcta tcccgccgcg cgcgacatcc gctacacgcc ggaaatgaaa
    23881 accgcaatgg tgctcaaggc aaaagacgtt atccgccgtg gctgtctgga atatgacgtc
    23941 agcgccaccg acatcaccag ctcgttcatg gctatccgca agaccatgac cagcagcgga
    24001 cgcagcgcca cctatgaggc cagccgcagc gaggaagcca gccacgccga cctcgcctgg
    24061 gcaaccatgc acgccctgtt aaatgagcca ctcaccgccg gtatcagcac tccgctgaca
    24121 tccaccattc tggagtttta ctgatgagca agaaaaaagg gaaaacaccg cgacctgcgg
    24181 caaaaacaat cactgccagc gccccgaaaa tggaagcatt cacctttggc gagccggtgc
    24241 cggtactcga ccgccgtgac attctggatt acgtcgagtg catcagtaac ggcagatggt
    24301 atgagccgcc ggtcagcttt accggtctgg caaaaagcct gcgtgctgcc gtgcatcaca
    24361 gctcaccgat ttacgtcaaa cgtaatattc tggcttcaac gtttatcccg cacccgtggc
    24421 tttcccagca ggatttcagc cgttttgtgc tggattttct ggtgttcggt aatgcgtttc
    24481 tggaaaagcg ctacagcacc accggtaagg tcatcaggct ggaaacctca ccggcaaaat
    24541 atacccgtcg tggcgtggaa gaggatgttt actggtgggt gccgtccttc aacgagccga
    24601 cagccttcgc gcccggctcc gtgtttcacc tgctggagcc ggatattaat caggagctgt
    24661 acggcctgcc ggaatatctc agcgccctta actctgcctg gctgaatgag tcggccacgc
    24721 tgttccgccg caagtattac gaaaacggcg cacatgccgg atacatcatg tacgtcaccg
    24781 atgccgtgca ggatcgcaac gatatcgaaa tgctccgcga aaacatggtg aagtcgaaag
    24841 gccgcaataa ctttaaaaat ctgtttctct atgccccaca ggggaaagcc gacggcatta
    24901 aaattatccc gctcagtgaa gtggcaacga aggacgattt ttttaatatc aaaaaagcca
    24961 gcgccgctga cctgctggac gcgcaccgca tcccctttca gttgatgggc ggcaagccgg
    25021 agaacgtcgg gtcactgggt gatattgaga aagtggcaaa ggtctttgtc cgcaatgagc
    25081 ttatcccgtt acaggacagg atccgcgaga taaacggctg gctcggtcag gaggtcatcc
    25141 gatttaaaaa ctactcactg gacactgaca acggctgaac atcgccgcct gcgggcggct
    25201 tttttacacc ccgccatcac gccctcacac gctcaccacc gcacaaaaca tcccgcagac
    25261 acacaacgcc ccggcaaaca atctaaacgc catcacgacg cgctcagacg ctgaaaaaat
    25321 aaaatcagca ccaccgccag cgcgcagtgc tttccccgcc tcgcccgccc gcttcatggg
    25381 gcgcttttaa tgcagtttca tgaatacgtt ggaagcgcgc cagacgtcac ggacaatact
    25441 tcaaataagt aaagctaatg catgcaaaaa aattcacgtc catgcataca ccagaaactg
    25501 ccatttataa ggatgatgta tgcagtagcc atataaaaag ctatactctt gccggtttat
    25561 cagggggaga aaatgccaaa agctgtatca ttgttctcgg gttgtggtgg ttccgatgct
    25621 ggattaagca aagctggttt cgacatcgtt atggcaaacg atattttgcc atatgcgaga
    25681 gaggtttatc tcgcaaacct accagaaaca gattacctaa tccaagatat ccgagaagtt
    25741 gaaaaatttc cgagtgcaga ccttcttgtt ggatgctatc catgccaagg atttagccaa
    25801 ggtggcgcac gtcttgcgga caggagtata aattttttat ataaagagtt tgcacgcgca
    25861 ttaaccaata ttcaacctaa agcatttatt gtcgaaaatg tttcaggtat gagaaatagt
    25921 acatttaagc atctactcga tgatcaaata aaacgcttct ccaatgctgg taatataggc
    25981 tataaagttg tttggaatat actcaaagct caccactacg gtgtaccgca agagcgtaag
    26041 agattaataa ttgtaggtat tcgtaacgac ttcgacctag aatataaatt ccctgaagcc
    26101 acacatggtg aggaaaaatc attaccttat gtaacaatcg gtgaagcact aaagggatta
    26161 cctgaatggc caatcggtga gttttgtgat gacccatttc actggtacta tttatctagg
    26221 aatagacgcc gcaactggga cgaagttagt aaaactatcg taagtaatct tcgtcatatt
    26281 cccttgcatc ccatcagccc cgaattaaaa aaagtgggtc tggacaagtg ggagtttata
    26341 acaaactcgc cagccagacg cttctcctat aaagaagcgg cgattctgca agggtttagt
    26401 aaagaatata ccaatcatgg tggttatcta acttttcctg acatggatag ttttggggcc
    26461 gcacggatgt taaaagagag atataaggtc gtaggtaatg cagttccacc acctttgttt
    26521 gaagcggttg tgagaaacat ccctgatata tggtaatggc ccatcactgg gcctttttta
    26581 attctttaca aaaggtaatt aacgcatcta actcatcctg catatcacca aattcatcct
    26641 taaaacgctc atatgcaata gttaaacgga ctcgatcaaa tataattcca gcttctgcgc
    26701 ttgcttctct tatcatttca tcaactaaca cataaggtgt tgagaaaact ttaacacact
    26761 taatgtaagt taatggatga aaccatggtg ttaacctttc aattttaata tcctgaaact
    26821 ttgttggcca atcattacca caagcgcatt gccctaaagc aaataaacgt ccatctctac
    26881 catccaaaaa atcaacccat gtaacaaaat ccacaccaca atctttaatt ctcaatgcat
    26941 cctcatctct taagccattc tctacgcgcc aagaccattc tcttgtattt ggtgagatcg
    27001 aagagttcaa tttataagct agttccttat aactactagg atttccattt gaacgaggcc
    27061 aaccagtatg catgtgtttt gcgtgtgaac taaaaaattt tgtgaataat cgcccagcca
    27121 taacttcaaa aaatctaggc aacttaacat tatctccttt tgttaaatta ttaattacag
    27181 agacacataa gcaaaacaga taaacaaggg aaggagactc ctttaatgtt agatgatttt
    27241 ctttatattc aaatggatac atatcatcca aaacctttgc tctccattgt atatgctcgg
    27301 caatctgacc aactaaaagc tcctttccct gatcttccat catccattct tcttccaaat
    27361 gaccatctaa attttctaac tcatcataga tagttccagc aatcttatca tcctcaattc
    27421 ctgagtctat tgtcgcgcta acatcaagta atgcattaaa ttctgcttga tccgcatctg
    27481 ttgcgaatcc ctgcatttta gagcgtaaac cataattcac aattaaaaac cctcactatc
    27541 acttccagta atagcctcat taattgatac tctaaaagat ctaaattgtt tacttatttg
    27601 ctcaagatat atattaagtt gaatagcatc ctgcattggt aaatcttcac gccctaccct
    27661 cgtaagacat tcgtccaatt tacttctaat ttctaacatt aaactagtca acctatcgcc
    27721 gattggctga gttaggtcat taatgtcttc aacatccttg ccttgcttga gcagtataac
    27781 tttttctgga gaagcaacta tctcagccaa gacaccaaga ttacgagagt ctttaacttg
    27841 tgagttttgt cctttatttt tattaccaaa catagctgtc attagaagct tcgcattatc
    27901 caatttcttt tcatctattg gattagggtc tgtaggatta tctgataagc ccacaaaatc
    27961 ctgaatactc ttataactca gcaatgtata aacccacgag aatggataac tagtattgct
    28021 ccctctccct ttcttcacac tatcatcttt attatacttc ccagcagact ccatttgttt
    28081 aatgaaatta tatccagaca aaagtctttt tatagtgcta ttacggtcac caatcatagt
    28141 ggaaatatca gatacgctca tatctccttc cttgatggtt cgagatatcc atgcagcctt
    28201 agcatatgaa tcccaatcct ttgtagatac gatatgcctc acacctaaat atgatataag
    28261 tgatttttta tctacaccat catcgtcttc aaaaactatt gctgggactg ggtctatttt
    28321 agggcaccca tgagaattgt atttatcaat atactgttga tgcagattaa cttgatctct
    28381 tgctctgtca tcatttttaa gtataagaca ggcagccagc cttctattac cttccattac
    28441 agtgaatttc ttattattat ttttcctcac caccattggc tcagcagaaa aataaccatt
    28501 cactgcaata gaacttatta catcagtaac gccataattt ttaacaatat tattcagcac
    28561 atcaacttgt gtatcagatg aagaagagtc ttcaccaaag cgggggtttt caacatcaag
    28621 ctccagatct tcaatattaa tatattgaac tgctttatgt tttaatttat cctccacacc
    28681 taccgagttt tttttcatat gtttacccct cccctaaatt ctggaatgca gtagtgctca
    28741 accctactct cgtttctgca cacctaacat acaacagcta gtttttccat gatagctttt
    28801 gctgtcgatg ctagacaagc caaagcctac agcggtctta tactattctg ttttaacttc
    28861 atacaatcct accgtttatt aactgattgt aaactcaagc accgcaatca aaaaaatcaa
    28921 cagaaggaaa tccaagccac tcatcagcga cctgataagt gaaatgtttc ccgtcataat
    28981 ttacggttgc cccacgcgcc agcgcctcaa gctcccatcg ctgcggcctg ataccgttct
    29041 gagcaaggtc aacgcggata cgggtaattt gcattcgctc cgaccgggtc agtctggccg
    29101 atggcgctat ttcatgcgct tttaacgggc ttccgtttct ttgctgacga tttggagttc
    29161 tcaggccgtg ttttaatgcg ccccttagcg ccctcacgac ctccgtgtca ttccattcga
    29221 taacaccact atcaaccaga ttaagcactg ctgcggcgtg ctcagaaggt gtgggagccg
    29281 gtaactcagt atcaccgccg atgagctttc cacagttatt gacaggactc cgaggcgcgg
    29341 cgatgccgct ttttaaagtc aaaggc
//
LOCUS       DAMZET010000040        28921 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.040, whole genome shotgun sequence.
ACCESSION   DAMZET010000040 DAMZET010000000
VERSION     DAMZET010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.040"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..653
                     /locus_tag="POB34_001867"
     CDS             <1..653
                     /locus_tag="POB34_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI5918830.1"
                     /translation="AGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQAIAQGIAM
                     VPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQLKVKTSSP
                     DLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQLVQQGIA
                     VIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHVEKQSV"
     gene            631..1812
                     /gene="xylH"
                     /locus_tag="POB34_001868"
     CDS             631..1812
                     /gene="xylH"
                     /locus_tag="POB34_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HDI5918831.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            1890..3068
                     /gene="xylR"
                     /locus_tag="POB34_001869"
     CDS             1890..3068
                     /gene="xylR"
                     /locus_tag="POB34_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HDI5918832.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(3176..4000)
                     /locus_tag="POB34_001870"
     CDS             complement(3176..4000)
                     /locus_tag="POB34_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HDI5918833.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            4320..6350
                     /gene="malS"
                     /locus_tag="POB34_001871"
     CDS             4320..6350
                     /gene="malS"
                     /locus_tag="POB34_001871"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDI5918834.1"
                     /translation="MKLAACFLTLLPGFAVATSWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASLVRECPKWDGLPLTVDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMT
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            6528..7781
                     /gene="avtA"
                     /locus_tag="POB34_001872"
     CDS             6528..7781
                     /gene="avtA"
                     /locus_tag="POB34_001872"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HDI5918835.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(7932..8405)
                     /locus_tag="POB34_001873"
     CDS             complement(7932..8405)
                     /locus_tag="POB34_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDI5918836.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVNAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(8507..9355)
                     /gene="yiaJ"
                     /locus_tag="POB34_001874"
     CDS             complement(8507..9355)
                     /gene="yiaJ"
                     /locus_tag="POB34_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HDI5918837.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFNIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            9556..10554
                     /gene="yiaK"
                     /locus_tag="POB34_001875"
     CDS             9556..10554
                     /gene="yiaK"
                     /locus_tag="POB34_001875"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HDI5918838.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            10566..11033
                     /locus_tag="POB34_001876"
     CDS             10566..11033
                     /locus_tag="POB34_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDI5918839.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            11151..11624
                     /gene="yiaM"
                     /locus_tag="POB34_001877"
     CDS             11151..11624
                     /gene="yiaM"
                     /locus_tag="POB34_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HDI5918840.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            11627..12904
                     /gene="yiaN"
                     /locus_tag="POB34_001878"
     CDS             11627..12904
                     /gene="yiaN"
                     /locus_tag="POB34_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HDI5918841.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            12917..13903
                     /gene="yiaO"
                     /locus_tag="POB34_001879"
     CDS             12917..13903
                     /gene="yiaO"
                     /locus_tag="POB34_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HDI5918842.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            13907..15403
                     /gene="lyxK"
                     /locus_tag="POB34_001880"
     CDS             13907..15403
                     /gene="lyxK"
                     /locus_tag="POB34_001880"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HDI5918843.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTVHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            15400..16062
                     /gene="ulaD"
                     /locus_tag="POB34_001881"
     CDS             15400..16062
                     /gene="ulaD"
                     /locus_tag="POB34_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDI5918844.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPAQVAGDFHAQIDAIWGGA
                     RA"
     gene            16055..16915
                     /locus_tag="POB34_001882"
     CDS             16055..16915
                     /locus_tag="POB34_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDI5918845.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTG
                     QSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            16909..17604
                     /gene="araD"
                     /locus_tag="POB34_001883"
     CDS             16909..17604
                     /gene="araD"
                     /locus_tag="POB34_001883"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDI5918846.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDDTRQWMVIKPS
                     GVKYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(17636..17737)
                     /locus_tag="POB34_001884"
                     /pseudo
     CDS             complement(17636..17737)
                     /locus_tag="POB34_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            17741..17929
                     /locus_tag="POB34_001885"
                     /pseudo
     CDS             17741..17929
                     /locus_tag="POB34_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17951..18691)
                     /locus_tag="POB34_001886"
     CDS             complement(17951..18691)
                     /locus_tag="POB34_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HDI5918847.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNKNT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            18815..19789
                     /locus_tag="POB34_001887"
     CDS             18815..19789
                     /locus_tag="POB34_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI5918848.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(19786..20922)
                     /locus_tag="POB34_001888"
     CDS             complement(19786..20922)
                     /locus_tag="POB34_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDI5918849.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(20928..21251)
                     /locus_tag="POB34_001889"
     CDS             complement(20928..21251)
                     /locus_tag="POB34_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDI5918850.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            21374..21577
                     /locus_tag="POB34_001890"
     CDS             21374..21577
                     /locus_tag="POB34_001890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918851.1"
                     /translation="MAFQQVEVYHIEIMELLTKLMNNSSKTSTVQIKRIKPSIIYRLL
                     LIGLGTPMVIYGLVCPLTIETRE"
     gene            complement(21796..23334)
                     /gene="aldB"
                     /locus_tag="POB34_001891"
     CDS             complement(21796..23334)
                     /gene="aldB"
                     /locus_tag="POB34_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HDI5918852.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(23499..24650)
                     /gene="yiaY"
                     /locus_tag="POB34_001892"
     CDS             complement(23499..24650)
                     /gene="yiaY"
                     /locus_tag="POB34_001892"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HDI5918853.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            complement(24840..26684)
                     /gene="selB"
                     /locus_tag="POB34_001893"
     CDS             complement(24840..26684)
                     /gene="selB"
                     /locus_tag="POB34_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDI5918854.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(26681..28072)
                     /gene="selA"
                     /locus_tag="POB34_001894"
     CDS             complement(26681..28072)
                     /gene="selA"
                     /locus_tag="POB34_001894"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDI5918855.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(28170..28778)
                     /locus_tag="POB34_001895"
     CDS             complement(28170..28778)
                     /locus_tag="POB34_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HDI5918856.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
BASE COUNT         7013 a         7602 c         7301 g         7005 t
ORIGIN      
        1 gtgccggacg taccgagacc attcagtgcc tgtttggcgt gtggcccgga caatgggaag
       61 gaaaaattta tattgatggc aaacaggtag atattcgtaa ctgtcagcaa gccatcgccc
      121 aggggattgc gatggtaccc gaagacagaa agcgcgacgg catcgttccg gtaatggcgg
      181 ttggtaaaaa tattaccctc gccgcactca ataaatttac cggtggcatt agccagcttg
      241 atgacgcggc agagcaaaaa tgtattctgg aatcaattca gcaactcaaa gttaaaacgt
      301 cgtcccccga ccttgctatt ggacgtttga gcggcggcaa tcagcaaaaa gcgatcctcg
      361 ctcgctgtct gttacttaac ccgcgcatcc tcattcttga tgaacctact aggggtatcg
      421 atattggcgc gaaatacgag atctacaaat taattaacca actcgtccaa cagggtattg
      481 ccgttattgt catctcttcc gaattacctg aagtgctcgg ccttagcgat cgggtactgg
      541 tgatgcatga agggaaacta aaagccaacc tgataaatca taacctgact caggagcagg
      601 tgatggaagc cgcattgagg agcgaacatc atgtcgaaaa gcaatccgtc tgaagtgaaa
      661 ttggccgtac cgacatccgg tggtttttct gggctgaagt cactgaattt gcaggtcttc
      721 gtgatgattg cagctatcat cgcaatcatg ctgttcttta cctggaccac cgatggtgcc
      781 tacttaagcg cccgtaacgt ctccaacctg ttacgccaga ccgcgattac cggcatcctc
      841 gcggtaggaa tggtgttcgt cataatttct gctgaaatcg acctttccgt cggctcaatg
      901 atggggctat taggtggcgt cgcggcgatt tgtgacgtct ggttaggctg gcctttgcca
      961 cttaccatca ttgtgacgtt ggttctggga ctgcttctcg gtgcctggaa cggatggtgg
     1021 gtcgcgtacc gcaaagtccc ttcatttatt gtcaccctcg cgggcatgtt ggcatttcgc
     1081 ggcatactca ttggcatcac caacggcacg actgtttccc ccaccagcgc cgcgatgtca
     1141 caaattgggc aaagctatct acctgccagc actggcttca tcattggcgc gcttggctta
     1201 atggcttttg ttggttggca atggcgcgga agaatgcgcc gtcaggcttt gggtttgcag
     1261 tctccggcct ctaccgcagt agtcggtcgc caggctttaa ccgctatcat cgtattaggc
     1321 gcaatctggc tgttgaatga ttaccgtggc gttcccactc ctgttctgct gctgacgttg
     1381 ctgttactcg gcggaatgtt tatggcaacg cggacggcat ttggacgacg catttatgcc
     1441 atcggcggca atctggaagc agcacgtctc tccgggatta acgttgaacg caccaaactt
     1501 gccgtgttcg cgattaacgg attaatggta gccatcgccg gattaatcct tagttctcga
     1561 cttggcgctg gttcaccttc tgcgggaaat atcgccgaac tggacgcaat tgcagcatgc
     1621 gtgattggcg gcaccagcct ggctggcgga gtgggaagcg ttgccggagc agtaatgggg
     1681 gcatttatca tggcttcact ggataacggc atgagtatga tggatgtacc gaccttctgg
     1741 cagtatatcg ttaaaggtgc gattctgttg ctggcagtat ggatggactc cgcaaccaaa
     1801 cgccgttctt gattttgata aaaattttct caaagccggt tacgtattac cggttttgag
     1861 tttttgcatg attcagcagg aaaagaacca tgtttactaa acgtcaccgc atcacattac
     1921 tgttcaatgc caataaagcc tatgaccggc aggtagtaga aggcgtaggg gaatatttac
     1981 aggcatcaca atcagaatgg gatattttca ttgaagaaga tttccgcgcc cgcattgata
     2041 aaatcaagga ctggttagga gatggcgtca ttgccgactt cgacgacaaa cagatcgagc
     2101 aagcgctggc tgatgtcgac gtccccattg ttggggttgg cggctcgtat caccttgcag
     2161 aaagttaccc acccgttcat tacattgcca ccgataacta tgcgctggtt gaaagcgcat
     2221 ttttgcattt aaaagagaaa ggcgttaacc gctttgcttt ttatggtctt ccggaatcaa
     2281 gcggcaaacg ttgggccact gaacgcgaat atgcatttcg tcagcttgtc gccgaagaaa
     2341 agtatcgcgg agtggtttat caggggttag aaaccgcgcc agagaactgg caacatgcgc
     2401 aaaatcggct ggcagactgg ctacaaacgc tgccaccgca aaccgggatt attgccgtta
     2461 ctgacgcccg ggcgcggcat attctgcaag tatgtgaaca tctacatatt cccgtaccgg
     2521 aaaaattatg cgtgattggc atcgataacg aagaactgac ccgctatctg tcgcgtgtcg
     2581 ccctttcttc ggtcgctcag ggcgcgcggc aaatgggcta tcaggcggca aaactgttgc
     2641 atcgattatt agataaagaa gaaatgccgc tacagcgaat tttggtccca ccagttcgcg
     2701 tcattgaacg gcgctcaaca gattatcgct cgctgaccga tcccgccgtt attcaggcca
     2761 tgcattacat tcgtaatcac gcctgtaaag ggattaaagt ggatcaggta ctcgatgcgg
     2821 tcgggatctc gcgctccaat cttgagaagc gttttaaaga agaggtgggt gaaaccatcc
     2881 atgccatgat tcatgccgag aagctggaga aagcgcgcag tctgctgatt tcaaccacct
     2941 tgtcgatcaa tgagatatcg caaatgtgcg gttatccatc gctgcaatat ttctactctg
     3001 tttttaaaaa agcatatgac acgacgccaa aagagtatcg cgatgtaaat agcgaggtca
     3061 tgttgtagcg atgatgagaa ttgtcggcgt cacatcaggg aactcagcac agccacctga
     3121 tgcgagcaaa agccttatcc gaccgacaaa tcagataagg ctcaaggaaa tgcaattaca
     3181 tatgcgccgc gattaaccgt tggttatcct ggtacattgc gaacaggtag ttgttataac
     3241 tcttcccttt ggtcgagtag cccttcagct tgtgaatcat cgctgtggca gtcacttcct
     3301 gatccgcttt acgcagctgc gcacgcgatt tacggaacga agagtaagcc ggatgcgtat
     3361 tgaggttagt gacataggcg ctcaccgatt ctttgacaga actaaactgt gagtaccctt
     3421 tcactttacc cggcgcattg gtacaacgtc ctttcatgca tttcatgccg aaaagattgt
     3481 tgttgttgcg cgccagcttc gacgttcccc aaccgctttc tgctgcagcc atcgtcgcca
     3541 ccatactggt agggataatg tctacgcgtt caagcaaggt attccacggg atttttcgcg
     3601 tattaccaga ccacttcacc ttgtagcgtt tagcgatgtc tttcagacgc gcacgctcag
     3661 caggtgacca ttggccctga tactgttttg aaatcagcca gttacgttct gcagtaatgg
     3721 ctgcattttg gctggtaatg taaggcatta cggtccggag aaacgccttt ttccttggtg
     3781 ttccggaagg gtattttcgc aaatcaggaa gtgaactact ctttgcacta ttgcgagaat
     3841 actcttgttt actgcttacc tgtttattac tagctttagt taagtgggac ttttgactcg
     3901 ctgttgttgt gtgcgtcttc gctaacacct cactcgaaaa caccagagtg agtaacataa
     3961 gaatcatcgc cccatatcgt cgtatgggag tcaaaatcat caggtctcct ggtcggattt
     4021 aatcattcca acaccttata tttttcacaa atttgagagt tgaatctcaa atcatatcaa
     4081 aaatagctgt caagagcacc ccaaggaata gtccaaatct gaaactatgt cacgagttaa
     4141 cgattcagat tggcgctaaa tcgcagaaaa tgtgggggtt atcgcaaaat tcagccgttt
     4201 tttgcgcgcg atcgctcacc cttgcttctc atcctgtgga cttaccgctc agggatgagt
     4261 tttgtctggc ttatcgctgg caaactgtct gaaatcgcag caataaggac tcatccgcca
     4321 tgaaactcgc cgcctgtttt ctgacactcc ttcctggctt cgccgttgcc accagctgga
     4381 cttctccggg gtttcccgcc tttagcgaac aggggacagg aacatttgtc agccacgcgc
     4441 agttgcccaa aggtacgcgt ccactaacgc taaattttga ccaacagtgc tggcagcctg
     4501 cggatgcgat aaaactcaat cagatgcttt ccctgcaacc ttgtagcaac acgccgcctc
     4561 aatggcgatt gttcagggac ggcgaatata cgctgcaaat agacacccgc tccggtacgc
     4621 caacattgat gatttccatt cagaacgccg ccgaaccggt agcaagcctg gtccgtgaat
     4681 gcccgaaatg ggatggatta ccgctcacag tggatgtcag cgccactttc ccggaaggag
     4741 ccgccgtacg ggattattac agccagcaaa ttgcgatagt gaagaacggt caaataacgt
     4801 tacaacccgc tgctaccagc aacggtttac tcctgctgga acgggcagaa actgacacat
     4861 ccgccccttt cgactggcat aacgccacgg tttactttgt gctgacagat cgtttcgaaa
     4921 acggcgatcc cagtaatgac cagagttacg gacgtcataa agacggtatg acggaaattg
     4981 gcacttttca cggcggcgat ttacgcggcc tgaccaacaa actggattac ctccagcagt
     5041 tgggcgttaa tgctttatgg ataagcgccc catttgagca aattcacggc tgggtcggcg
     5101 gcggtacaaa aggcgatttc ccgcattatg cctaccacgg ttattacaca caggactgga
     5161 cgaatcttga tgccaatatg ggcaacgaag ccgatctacg gacgctggtt gatagcgcac
     5221 atcagcgcgg tattcgtatt ctctttgatg tcgtgatgaa ccacaccggc tatgccacgc
     5281 tggcggatat gcaggagtat cagtttggcg cgttatatct ttctggtgac gaagtgaaaa
     5341 aaacgctggg tgaacgctgg agcgactgga aacctgccgc cgggcaaacc tggcatagct
     5401 ttaacgatta cattaatttc agcgacaaaa caggctggga taaatggtgg ggaaaaaact
     5461 ggatcagaac ggatatcggc gattacgaca atcctggatt tgacgatctc actatgtcgc
     5521 tggccttttt gccggatatc aaaaccgaat caactaccgc ttctggtctg ccggtgttct
     5581 ataaaaacaa aacggatact cacgctaaag tcatcgaagg ctttacacct cgcgattact
     5641 taacccactg gttaagtcag tgggtccgcg actatgggat tgatggtttt cgggtcgata
     5701 ccgccaaaca tgttgagttg cccgcctggc agcaactgaa aaccgaagcc agcgccgcgc
     5761 ttcgcgaatg gaaaaaagct aaccccgaca aagcattaga tgacaaacct ttctggatga
     5821 ccggtgaagc ctggggccac ggcgtgatgc aaagtgacta ctatcgccac ggcttcgatg
     5881 cgatgatcaa tttcgattat caggagcagg cggcgaaagc agtcgattgt ctggcgcaga
     5941 tggatacgac ctggcagcaa atggcggaga aattgcaggg tttcaacgtg ttgagctacc
     6001 tctcgtcgca tgatacccgt ctgttccgtg aagggggcga caaagcagca gagttattac
     6061 tattagcgcc aggcgcggta caaatctttt atggcgatga atcctcgcgt ccgttcggtc
     6121 ctacaggttc tgatccgctg caaggtacac gttcggatat gaactggcag gatgttagcg
     6181 gtaaatctgc cgccaacgtc gcgcactggc agaaaatcag ccagttccgc gcccgccatc
     6241 ccgcaattgg cgcgggcaaa caaacgacac tttcgctgaa gcagggctac ggctttgttc
     6301 gtgagcatgg cgacgataaa gtgctggtca tctgggctgg gcaacagtga cttttccggc
     6361 ttcccgttcg tcagtacctc gggaagccgc caaccaggat aaaatgtcag ccctaatcag
     6421 cgttgcagga taaagcaccg ctcactctcc aacagaccga tttgcacccc agcaaatgta
     6481 gcgttattgt taccttcctt gctacagagt tcgacagata tcccgctatg acattctccc
     6541 tttttggtga caaatttacc cgccactccg gcattacgct gctgatggaa gatctgaacg
     6601 acggtttacg cacgcctggc gcgattatgc tcggcggcgg taatccggcg cagatcccgg
     6661 aaatgcagga ctacttccag acgctactga ccgacatgct ggaaagtggc aaagcgactg
     6721 atgcactgtg caattacgac ggtccacagg ggaaaacgga gctactcaca ctgcttgccg
     6781 gaatgctgcg cgagaagttg ggttgggata tcgaagcaca gaatattgca ctaacaaacg
     6841 gcagccagag cgcgtttttc tacttattta atctgtttgc cggacgccgt gccgatggtc
     6901 gggtcaaaaa agtgctgttc ccgctggcac cggaatacat tggctacgct gacgccggac
     6961 tggaagaaga tctgtttgtc tctgcgcgtc cgaatattga actgctgccg gaaggccagt
     7021 ttaaatacca cgtcgatttt gagcatctgc atattggcga agaaaccggg atgatttgcg
     7081 tctcccggcc gacgaatcca acaggcaatg tgattactga cgaagagttg ctgaagcttg
     7141 acgcgctggc gaatcaacac ggcattccgc tggtgattga taacgcttat ggcgtcccgt
     7201 tcccgggtat catcttcagt gaagcgcgcc cgctatggaa tccgaatatc gtgctgtgca
     7261 tgagtctttc caagctgggg ctgcctggct cccgctgcgg cattatcatc gccaatgaaa
     7321 aaatcatcac tgccatcacc aatatgaacg gcattatcag cctggcacct ggcggtattg
     7381 gtccggcgat gatgtgtgaa atgattaagc gtaacgatct gctgcgcctg tctgaaacag
     7441 tcatcaaacc gttttactac cagcgtgttc aggaaactat cgccatcatt cgccgctatt
     7501 taccggaaga tcgctgccta attcataaac cagaaggagc cattttcctc tggctatggt
     7561 ttaaggattt gcccattacg accgagcaac tctaccagcg cctgaaagca cgcggcgtgc
     7621 tgatggtgcc ggggcacaac ttcttcccgg gactggataa accgtggccg catacgcatc
     7681 aatgtatgcg catgaactac gtaccagagc cggagaaaat tgaggcgggg gtgaagattc
     7741 tggcggaaga gatagaaaga gcctgggctg aaagtcacta aaaattgcct gatgcgctac
     7801 gcttatcagg cctacataat ttctgcaata tattgaattt ggaagaattt gtaggccgga
     7861 taaggcgttt acgccgcatc tggcataaac aaagcgcact attccggcgt tgagaaacgc
     7921 cggaaaacgt ttcaaacagg ctgctgccgt agccgatgcc gctgcacttt ctcgacatcc
     7981 atgcactgca acgcctttgt cgggcaggct tcaacacagg ccggccccgt ctcccgatgc
     8041 cagcacagat cgcacttaat cgccctcgcc tttttgcgcg aagaaacgac ctccatcgca
     8101 ccaaacgggc aagccagcat acagctttta cagccaatgc aacgtgtttg ttcaacgaaa
     8161 atatgcccat gttcgcggct tatcgcgtta acagggcaga cattcgcgca cggtgcatct
     8221 tcacactgat gacaggctac tgccgtggtc cagctgtggt ctttaatgac acgaatacgg
     8281 gaaataaact cgtctggtga caacgcagcg caatcctgat tctcctgatg cgacactgcg
     8341 caagccactt cacaggtacg gcaaccgata catttcgtcg catccgcaat aataaaccgg
     8401 ttcatgatca ctcctttgca ctaaaacagc agagtgccag agagcgcgtc cctactgctt
     8461 tgatccaacg aaggaaatgt taaagttttg gcacttgtcc aaagcgttat gtgatcgcgc
     8521 cctggtcgtc cctgacagta aatcccagtt cattagaaat agcctgcgcg gtttcacgca
     8581 gtggtttcag gagatttttc tcccccacct gtttcagacg tgatgtcgaa agcgaaatcg
     8641 acacggcgta cggcacccgc ccatgaatat taaacaccgg aacagcaata caggagacgc
     8701 cgagttcgtt ttcttctctg tccatcgccg ctccgctttc acgaatgtgc gccagttcgt
     8761 cgaacatcgc gggcagctcg gtaatggtat tgcgggttaa cggctggatc tcatgctgat
     8821 ggttttccca gtatgacttc acgtagtccg ggtgaccaaa cgccatgtag atcttgccca
     8881 ttgcggaaca gtagagcggc atatgctggc caatataggc acgggttcgc agcatcccgg
     8941 ttgtgggttc cagcttataa atcaaaatag cgtgatcgtc ttcgcggctg gagaagttaa
     9001 tggtttcacc agtggcgatg ttcagtgcct caagatgcgg agcggcgata tgaatgatat
     9061 tcagcgaaga cagcgccttc tgcccgacgg caataaattt ggtagtcagg cggtaactcc
     9121 ctgcggcggg cgcggtggtc acatagccgc aggactgtaa tccctgcaat aagcgatgga
     9181 cggtactctt atttaaacca gccagctccg aaagatgcgc caacggacaa ccgtttggat
     9241 agttgctcaa aatctcaatc agcatcaacc cgcgaaacag actctggctt ccggctggac
     9301 gctctttttc ctgcgccatc tcgttctctt tttttcccat cacttctttc cccattttgt
     9361 cgcgtcctga tggtagcgca aagtgtgccg tagttcacga tctcgacaga taatttataa
     9421 ccacttgatt tttatgtctt ttgaaattca tcaatcagat tgctttgtta aaaagcgatc
     9481 gatatacttg aaatcaagtt tcgcatattg aaattttaag ccaaaaaagc gatcaaaaaa
     9541 acaaggaagc ctgggatgaa agtgacattt gagcagttaa aagcagcctt taatcgggtc
     9601 ttaatttcac gcggcgttga cagcgaaacg gctgacgcct gtgcagagat gttcgcccgc
     9661 accaccgaat ccggcgttta ttctcacggc gttaatcgtt tccctcgttt cattcaacaa
     9721 ctggaaaacg gcgatatcat tcctgatgcc caacccaaac gtataaccag cctcggcgca
     9781 attgaacagt gggatgccca gcgttcgatc ggcaacctga cggcgaaaaa gatgatggat
     9841 cgcgccattg aactggctgc cgatcacggt attggtctgg tggcacttcg taatgccaac
     9901 cactggatgc gcggcggcag ctacggctgg caggcggcgg aaaaaggcta tattggcatt
     9961 tgctggacca actccatcgc cgtaatgccg ccgtggggcg caaaagagtg tcgcattggc
    10021 actaacccgc tgatcgtcgc cattccttcc acgccgatca ccatggtcga tatgtcgatg
    10081 tcgatgttct cttacggcat gttagaagtt aaccgcctgg cgggccgtca gctcccggtc
    10141 gatggtggct ttgatgatga gggcaatttg accaaagaac ctggcgttat cgagaagaat
    10201 cgccgcattt tgccgatggg ctactggaaa ggttctggca tgtcgattgt gctggatatg
    10261 atcgctactc tcctttccga cggcgcatcg gttgccgaag tcacccagga caacagcgac
    10321 gaatacggca tttcacaaat ttttattgcc attgaagtgg acaagcttat cgacggtccc
    10381 acccgcgatg ccaagctgca acgtatcatg gattacgtta ctaccgctga acgcgctgac
    10441 gaaaaccagg ccatccgctt acccggccac gaatttacta ccctgctggc cgaaaaccgc
    10501 cgtaacggta ttaccgttga tgacagcgtg tgggccaaaa tccaggcgtt ataaggagat
    10561 aagtcatgat ttttggacat atcgcgcagc ccaatccgtg ccgtttgccc gccgccattg
    10621 aaaaggcgct cgattttctg cgcgctaccg atttcaacgc cctggagccg ggcgttgtcg
    10681 aaatcgacgg caaaaatatt tatgcgcaaa ttatcgattt aaccacacgc gaagcggtgg
    10741 aaaaccgtcc ggaagtgcat cgtcggtata tcgatattca gtttctggcg tggggtgaag
    10801 agaaaatcgg cattgctatt gatacgggaa ataataaagt cagcgaatca ctattagagc
    10861 agcgcgatat tattttttat catgacagtg aacatgaatc atttatagaa atgataccgg
    10921 gcagctacgc aattttcttt ccgcaggatg ttcaccgacc aggttgtatt ttgcaaacag
    10981 cctctgagat acgaaaaatt gtggttaaag tcgcattaac ggcgctgaat taatcgaata
    11041 atcgtctacg ccagaacgac tgggtaatgt tattgctcta actatattaa taatttaggt
    11101 tgctgcacat gcaacttgaa ttatgacaga catagcctca aggaatagct atgaaaaaaa
    11161 tacttgaagc aatactggcg attaatctcg ccgtactttc ctgtattgtg tttataaaca
    11221 ttattttaag atatggtttt cagacaagca ttttatctgt agatgaattg tcacgttatt
    11281 tatttgtctg gttaacattt attggcgcga ttgtggcttt tatggataac gcccacgttc
    11341 aggtcacctt tctggtggag aaactctccc ccgcatggca acgacgagtc gctctggtta
    11401 cccattcctt aatcttattt atttgtggcg cactggcctg gggcgcaacg ctaaaaacaa
    11461 ttcaggactg gagcgattat tcaccgatcc tcggtttacc catcggcctg atgtatgccg
    11521 cttgtctgcc caccagcctc gttatcgcat tctttgaact gcgtcattta tatcaactca
    11581 tcacgcgtag caattctcta acgtcgccac cgcaaggagc ttaatcatgg ctgtgctgat
    11641 ttttctgggc tgtctgttgg gtggtattgc tatcggtttg cctattgcct gggcactgtt
    11701 gttgtgcggg gcggcgttaa tgttctggct ggacatgttt gatgtccaga tcatggcaca
    11761 aacgctggtg aacggagccg atagcttctc cctgctggcg attccgttct ttgtgctggc
    11821 gggtgaaatc atgaatgcgg gcggcttgtc aaaacgcatt gttgacctgc cgatgaaact
    11881 ggtggggcat aaaccaggcg ggctgggcta cgtcggcgtg ctggcagcta tgattatggc
    11941 gagcctttct ggttctgccg ttgcggatac cgccgccgtt gccgctctac tggtgccgat
    12001 gatgcgcagc gccaactacc cggtcaaccg ggcggcgggg ctgattgcct ctggcggcat
    12061 tatcgcgcca attattcccc cttccattcc gtttattatc ttcggcgttt ccagcggatt
    12121 atccatcagt aagctgttta tggcgggcat tgcccctggc atgatgatgg gcgcaacgct
    12181 gatgcttacc tggtggtggc aggccagtcg ccttaattta ccccgccagc aaaaagcaac
    12241 gatgcaggaa atctggcact cttttgtctc cggtatctgg gcgctgtttc ttccagtcat
    12301 cattattggc gggtttcgct ccggtctgtt tacccctacc gaagccgggg ccgttgctgc
    12361 tttctatgcg ctgtttgtcg ccacagttat ttaccgtgaa atgaccttcg ccacactttg
    12421 gcatgtactc attggcgcgg cgaaaaccac ctcagtggtt atgtttctgg tggcctcggc
    12481 acaagtttcc gcctggctga ttaccattgc tgaactgccg atgatggttt ctgatttact
    12541 gcaaccgttg gtcgattcac cgcgtctgct gtttatcgtc attatggtgg ctatcctgat
    12601 tgtcggcatg gtcatggatt taacgccgac ggtattaatt cttaccccgg tattaatgcc
    12661 tttagttaaa gaggcaggaa tcgatccgat ttatttcggt gtcatgttta tcattaactg
    12721 ctcaatcggc ttaattacac cgcctatcgg taatgtactt aacgttattt caggggtggc
    12781 aaaactcaaa ttcgatgatg cagtcagagg cgttttccct tacgtcctgg ttttatattc
    12841 attattagtc gtatttgttt ttattcccga tctcatcatc ctccctttga aatggattaa
    12901 ttaaaaggaa aatattatga aattacgctc tgtaacctac gcattattca ttgctggcct
    12961 ggctgcattc agcacatctt ctctggcggc gcaatcttta cgtttcggtt atgaaacctc
    13021 acaaaccgac tcgcaacata ttgcggcgaa aaaattcaat gatttattgc aggagagaac
    13081 caaaggcgag ctgaaattaa aattgtttcc ggatagcacc ctcggtaacg cgcaggcgat
    13141 gatcagcggc gtacgtggcg gcaccatcga tatggaaatg tccggctcga ataactttgc
    13201 cgggttatca ccagtgatga acttgcttga tgtccctttc ctgttccgcg ataccgctca
    13261 cgcgcataaa acgctcgacg gcaaagtcgg tgatgatctg aaagcctcac ttgaaggtaa
    13321 aggactaaaa gtactggcct actgggaaaa cggctggcgc gatgtcacca actcgcgcgc
    13381 accggttaaa acccccgccg acctgaaagg gctgaaaatt cgcaccaaca atagcccgat
    13441 gaatatcgcc gcattcaaag tctttggcgc taacccgatc ccgatgccgt ttgccgaagt
    13501 ctataccggg ctggaaaccc gcactatcga cgctcaggaa cacccgatca acgtcgtctg
    13561 gtcagcaaaa tttttcgaag tgcagaagta cctttctctg acgcaccacg cctattcccc
    13621 gcttctggtg gtgatcaaca aagcgaagtt tgatggctta accccggagt tccagcaagc
    13681 gctaatttca tctgcgcagg aagcgggtaa ctaccagcgc aaactggttg ccgaagatca
    13741 gcaaaaaatc atcgacggca tgaaagaagc gggcgtggaa gtcatcaccg atctcgaccg
    13801 caaagccttt agcgacgcac tgggtactca ggtccgcgac atgtttgtaa aagatgttcc
    13861 gcagggtgct gatctactga aagccgtgga tgaggtgcaa taaaccatga cgcaatactg
    13921 gctggggtta gattgtggcg gtagctggct gaaagccggg ctgtatgacc gcgaaggccg
    13981 ggaggcaggc gtgcagcgcc tgccgctgtg cgcattaagc ccgcagccag gttgggcaga
    14041 gcgcgatatg gcagaactgt ggcaatgctg catggctgtc attcgcgaac tgcttaccca
    14101 ttccggcgtt agcggggaac aaattgtcgg tatcggtatc tccgcacaag gaaaaggctt
    14161 gtttttgctg gataaaaaca acaagccgct cggtaacgcc attttgtcct cggatcgccg
    14221 ggcgatggaa atcgttcgtc gctggcagga agatggcatc ccggaaaaac tctacccgtt
    14281 gacccgacaa accttgtgga ccgggcatcc ggtgtcgctg ttacgctggc tgaaagagca
    14341 cgaaccggaa cgctacgcgc aaattggctg cgtgatgatg acgcacgact acctgcgctg
    14401 gtgtttaacc ggcgtcaaag gctgtgaaga gagcaatatt tccgagtcca acctctacaa
    14461 catgagtctt ggggaatatg acccgtgcct caccgactgg ctggggattg ctgaaatcaa
    14521 tcacgccctg ccgcctgttg tcggatctgc cgaaatctgc ggggagatca ccgctcagac
    14581 agccgtactg accggtctga aagcgggtac gcccgttgtc ggcggcctgt ttgatgtggt
    14641 ttccaccgca ctctgcgccg ggatcgaaga cgaatttacc cttaatgcgg tgatgggcac
    14701 ctgggcggtg accagcggca taacccgcgg tttacgtgac ggtgaagcgc atccgtatgt
    14761 ctatggtcgc tacgttaacg atggtgaatt tatcgttcac gaagccagcc ccacctcttc
    14821 cggcaacctc gaatggttta ccgcacagtg gggagaaatc tcgtttgatg agatcaacca
    14881 ggccgttgcc agcttgccga aggccggtgg cgatctcttt ttcctgccgt tcctgtacgg
    14941 cagcaacgcc ggactcgaga tgaccagcgg tttctacggg atgcaggcca ttcacacccg
    15001 cgcacacctg ttgcaggcca tttatgaagg ggtggtgttc agccatatga cccatctcaa
    15061 ccggatgcgc gaacgtttta ctgatgtgca caccctgcgc gtcactggcg gcccggcgca
    15121 ttccgatgtc tggatgcaaa tgctggcgga cgtcagcggt ctgcgtattg agctgccgca
    15181 ggtggaagaa accggctgct ttggtgcagc acttgccgcc cgcgtcggca ccggggttta
    15241 tcgcgatttc agcgaagccc agcgtgattt acagcacccg gtgcgcaccc tgctgccgga
    15301 tatgactgtc catcagcttt accaacaaaa ataccaacgc tatcagcatc tcattgccgc
    15361 acttcagggc tttcacgccc gcatcaagga gcacatatta tgagccgacc acttctgcaa
    15421 ctggcactcg accactcatc acttgaagcc gcacagcgcg acgtgacgca gttaaaagac
    15481 agcgtcgata ttgtcgaagc gggcaccatt ctctgtttaa acgaagggct tggcgcggtg
    15541 aaagccttgc gcgaacagtg cccggacaaa atcatcgttg ctgactggaa ggtcgccgac
    15601 gctggtgaaa cgctcgcgca acaggcgttt ggcgcaggcg ctaactggat gaccatcatc
    15661 tgcgccgcgc cgctcgcgac ggtagaaaaa ggccacgcaa tggcacaacg ctgcgggggt
    15721 gaaattcaga tagagctgtt cggtaactgg acgctggacg acgcccgcga ctggcatcgt
    15781 attggcgtgc ggcaggccat ttatcatcgc ggtcgtgatg cacaggccag cgggcaacag
    15841 tggggcgaag ccgatctggc acgcatgaag gcgctttcag atatcggcct tgagctttcc
    15901 attactggcg ggattacccc tgctgacctg ccgctgttta aagatatccg cgtgaaagcg
    15961 tttattgccg ggcgggcact ggcaggcgcg gcaaatccgg cgcaagtggc tggcgatttc
    16021 catgcgcaaa tcgacgctat ctggggaggt gcgcgtgcgt aatcatccgt tagggattta
    16081 tgaaaaagcg ctggcgaaag atctctcctg gccggagcgg ctggtgctgg caaaaagctg
    16141 cggttttgat tttgtcgaaa tgtcggtgga tgaaaccgac gaacggctct cacgtcttga
    16201 ctggagcgcc gcacaaagga cttcgctggt tgccgcgatg atcgaaacag gtgttgggat
    16261 cccgtcgatg tgcctgtccg cacatcgtcg ctttcccttt ggtagccgtg acgaggcagt
    16321 gcgcgaacgg gcgcgggaaa tcatgagtaa agcgattcgc ctggcgcgcg atctcggcat
    16381 tcgcaccatc cagctggcag gttatgacgt ctattacgaa gaccacgacg aaggcacccg
    16441 gcaacgtttt gctgaagggc tggcgtgggc agtcgaacag gcggcggcat cacaagtaat
    16501 gctggcggtg gagattatgg ataccgcgtt tatgaactcc atcagcaaat ggaaaaaatg
    16561 ggacgagatg ctcgcctcgc cgtggttcac cgtctacccg gacgtcggca acctcagcgc
    16621 ctggggcaat gatgttcccg ccgaactgaa actgggcatt gaccgtatcg ccgcgatcca
    16681 cctgaaagac acccagccag tcaccgggca aagccccgga cagttccgtg atgtgccgtt
    16741 tggcgaaggc tgcgtcgatt tcgttggcat cttcaaaacg ctgcatgaac tgaactatcg
    16801 cggttctttc ctgattgaga tgtggaccga aaaagccaaa gagccggtgc tggagatcat
    16861 tcaggcgcgg cgctggattg aagcgcgtat gcaggaggct ggatttatat gttagagcaa
    16921 ctgaaagccg acgtgctggc ggcgaatctg gcgcttcccg ctcaccatct ggtgacgttc
    16981 acctggggca atgtcagcgc ggtagacgac acgcggcaat ggatggtaat caaaccttcc
    17041 ggcgtcaagt acgacgtgat gaccgccgac gatatggtgg tggttgagat agccagcggc
    17101 aaggtggtgg aaggtagcaa aaagccctcg tccgatacgc caacgcatct ggcactttac
    17161 cgtcgctatg ccgaaattgg tggcattgtg catacccact cgcgccacgc caccatctgg
    17221 tcacaggccg ggctggatct ccccgcctgg ggcaccaccc acgccgatta tttttacggt
    17281 gctatcccct gcacgcgaca gatgaccgca gaggagatta acggcgaata tgaatatcag
    17341 accggcgaag tgatcattga aaccttcgaa gaacgtggca ggagtccggc acaaatcccg
    17401 gcggtgctgg tgcattctca cggcccgttc gcatggggta aaaacgccgc cgatgccgtg
    17461 cataacgccg tagtactcga agaatgcgcc tatatgggtc tattctcgcg ccagcttgcg
    17521 ccgcagctcc ctgcgatgca aaacgaactg ctggataagc actacctgcg taagcatggg
    17581 gccaatgcct attacgggca gtaatccctc acgccggggc ttcatcgccc cggcactacg
    17641 aattgatatg ttccttgctg taacgccgct tccacgctgc tggcgttaaa ccagtatgtt
    17701 tctgaaaaat ctgccggaaa tagccaacgt cattaaaccg tattgcatgg agcaggccga
    17761 caacgtcgag tcactgcata acctgttgct gcccgccgat gcgccattta cgacatttga
    17821 aggcaaggga ttgttcagcc ataagatcct gattcagacg ccaggttccc ggtatgaaca
    17881 gagcaatcca gagcaacaac ctttgtggca ttacgacgga gcaccagccg caccgacatc
    17941 caccggtgaa ttaaaaacgc cagctcaccc cggtgacaaa ggtaaaactc tcattacgat
    18001 caatcatcgg gctgtcggta atctcttccg gcagctcgct gtatcctgcg ctcgccatca
    18061 atacgacgtg ctctcctatc gggtattttg ccgtcaggct gacatagggc acccaggcat
    18121 cctgcgcgga ataacttgcc agaccgctac gacgggactc actctctgaa atgccatagt
    18181 aatagtcact gaaattctcg tcataataga gaacgcccag cgcaggtgtc agcgacaggc
    18241 gacctatctg cattttgtgg aataccgata gctcccccac ccagccgttg ctgttatcca
    18301 gaacgtccgc agccgcagag gctttcacgg ttccccaacg ctcgtggtga taccaggcaa
    18361 cccccgccat agccgtacta tcacgcttat caagctgttg catggcataa tcgtcattat
    18421 cggcagggtc aaattccagc ggcatccagg atgcggtcag gctaagttca tttttttcac
    18481 tttgcgacag aataaaacct aacgtggtct gacgaacata aaaagaatca ccttcataac
    18541 taatcagcgg aattgccttt gtatttttat tataaccgcg ataaggcgat tcattataag
    18601 ccgcaccagc accaatggat aattcagaag cagttgcgct tgccataaaa ggcaacgcaa
    18661 ataacgccac aatattgcgg ttaattaaca tgtccggtat tccattttaa aataagataa
    18721 aaaagagtcg gcatagtaat gcttacaatt tcttcggtgc aagattcata tttatatagt
    18781 aatatataaa attatatata attgggctgt tgcgatgaca aaattacaac ttaagtaccg
    18841 ggagttaaaa attatctcgg taatcgctgc cagtgaaaat atcagccatg ccgcgactgt
    18901 acttggcatc gcacaggcca acgtcagcaa ataccttgct gattttgaat caaaagtggg
    18961 tttaaaagtc tttgaccgaa ccacccggca gctgatgctc acaccttttg gtaccgcgct
    19021 gttaccgtac atcaacgaca tgctggacag aaacgagcag cttaataatt ttattgcaga
    19081 ttataagcat gagaaacgtg gtcgggtcac catctacgcc ccaaccggca taattaccta
    19141 tttatccaaa catgtaatcg ataaaattaa agatatcggt gatatcacct tatcgctaaa
    19201 aacctgcaat cttgagcgta atgcctttta tgaaggcgtc gaatttcctg atgattgtga
    19261 tgttttaatc agctacgctc caccaaaaga tgaatcgctg gttgccagtt ttattaccca
    19321 atatgccgta accgcttatg ccagccagcg ctatcttgag aaacatccta ttagccgtcc
    19381 tgacgaactc gaacatcact cctgtatttt gatcgactcg atgatgattg atgacgcgaa
    19441 tatctggcgc ttcaacgtcg ccgggagtaa agaggtgcgg gattatcgcg tgaagggaaa
    19501 ttacgtttgc gataacacgc aatcggcgct ggagctggca cgaaatcacc tggggattgt
    19561 gtttgcgcca gacaaaagtg tgcagagcga tctgcaagac ggcacgctgg tgccctgctt
    19621 tcagcacccg tatgaatggt ggctggatct ggtggctatc ttccgtaaac gggaatacca
    19681 gccctggcgg gtccagtatg tgctggatga aatgctgcgt gaaattcgcc accagcttgc
    19741 tcagtcgcag caactgcggc ctgaacaagc ggcggaaagc gaagattaat gatcgagata
    19801 aaggtaatgc acccaactgg tcatgcgtaa cagcactttg cgcatcaccg agacatggct
    19861 gaaatgatca gagtagaccg ccacctgggc gtaaataccg tctggtaaag cgctcaaatc
    19921 agtgtgctca tccagttcaa tggtcgcgat aacgccatct gaacccggcg ctgtgtttaa
    19981 cgtctgtaag gtgccggtcg actgataagc tccgccggga acagctggac taatggctgc
    20041 cagcttaccg ctgaacactt tgcctggcag agcgttaaat accacttccg catcatcgcc
    20101 gggagccagg cgcagcaagg agttctgacg gaactgcgcc acgatttgtc gtttctgatc
    20161 gggtataaac accatcaccg gacgtagcgg cagcgacgcg gcataggtac ccggacgaat
    20221 cagcacctgg gtcacataac catcgctcgg cgcacgcact atcgtctgct caaggttata
    20281 ttttgcttcc gcgagctgcg ctttcaggct ggcgatttga gaatgttcac ccaacaccag
    20341 gctatccagc tggctctgga tctgtttttg ttccgccgcc gatgacttta cggaggcttc
    20401 ctgcgccaga taattttgcc gcgccacatc aatatcgcgt tctgaaaacg ggtttacttt
    20461 cgcctggctg ccgcgtgcgt aacgctgata ctctttagcg aatttatccc gcgtggcctt
    20521 tgcctgctga gtattcgccg ccatctcatc taactctgcg cccaacgccc gctgtttatg
    20581 ttctgcggta acgatatccg ccatcagccg atccacccgc gcctgataac gcgtcgggtc
    20641 cagtcgaaat agcacctcac cttttttaat cagcgtattt ttcttatccg tcacttcgat
    20701 caccacgcct gtcacctgtg ggacaacagg aatagaaata accgcttttt gcgctttgaa
    20761 ggtatacgga tggttatagt tcattaacag aattaaacca ctgacaataa atatgccacc
    20821 cagggccgct gtgggaatgg tccatttatt tacaggaatt ttgaagatct taaacattgc
    20881 ccatgcaaaa gccacatagg tcaaaataat cagtaaatcc ataagtatta ctcggcagaa
    20941 gaggatttga tgtcggcgag ctgtttctcc agcccggcaa tacgctgttg tagttgcaca
    21001 acggaggagt catggttttg cattccccat ccccgctccg ggcggtaaag cgtggcccaa
    21061 atccacaaaa atggccagat aacatgcagc gtaaaaagac tcacccaacc agcaacatga
    21121 atggcgtcgg catgaggatg attacgtttt ttagcaatca ggtagggaat atcatgtaaa
    21181 ataataatcc catagaatat caccagaaat acaaaaataa gcactcccag tgcaaaataa
    21241 tccagaaaca tatttccctc agagaatata aaaaagaaca attaacgcat attatgaaat
    21301 cccatgatgc agtgcaattt ccttatttat ataacggcat attgtttttt atatactttc
    21361 tgaagtgtgc tgcgtggctt ttcaacaggt cgaggtttat catattgaaa taatggaact
    21421 attgaccaag ctaatgaata actccagtaa aacatccaca gtacagataa agcgtattaa
    21481 accttcaatt atctaccgtt tattgctgat tggcctcgga acgccaatgg tgatttacgg
    21541 cctagtttgc ccgctcacca tcgaaacgcg agagtaaact cctggcaaaa cacgccccag
    21601 aaacgcactt ctgttggcgt agtctgggtt attgcgcacg taggtttaaa acgtaaattg
    21661 taacaacgtg cgctttgttt atgccggatg cggcgtgtac gccttatccg gcctacggtc
    21721 aggttcccgt aggcatgata agacgcgtaa gcgtcgcatc aggcaatgaa tacccaatgc
    21781 gaccagcttc ttatatcaga acagccccaa cggtttatcc gagtagctca ccagcaggca
    21841 cttggtttgc tggtaatgct ccagcatcat cttgtgggtt tcgcgaccga tacctgattg
    21901 tttgtagcca ccaaacgcag catgtgccgg gtaagcgtga tagcagttgg tccacacgcg
    21961 cccagcctgt atgccgcgcc ccatcttata ggccagatta ccgttgcggc tccagacgcc
    22021 cgcgcccagg ccatattgcg tatcgttcgc cagctccagc gcttcttcca tcgttttgaa
    22081 ggtggtcacc gccagcaccg ggccaaaaat ctcctcctgg aacacccgca tattgttctg
    22141 accaaacaga atcgtcggtt cgaggtagta gccgtctttc agttcacctt ccagcagctt
    22201 gcgccgcccg cctgtgagca cgtcagcgcc ctctttttta ccgatatcaa tgtagttgag
    22261 gatggtttcc agttgcccgt gagaaacctg cgcgcccatt tgcgtcacgc tgtcgagcgg
    22321 gttaccgctg cgaatgcttt cgacacggcg gatggcgcgt tccataaagc gttcgtagat
    22381 agattcctgc actaaagcac gactcggaca ggtgcaaact tcgccctggt taaaggcaaa
    22441 cagtgcaaag ccttccagcg ctttatcgaa aaaggcatct tcttcatcca tcacatcagc
    22501 aaagaagata tttggcgact taccgcccaa ctccagcgtc accggaataa tgttttgcgt
    22561 tgcgtattgc ataatttgtt ggcccacttc cgttgagccg gtaaacgcca ctttggcgat
    22621 gcgtttcgag gtcgccagat attcgccaat ttccccacct gcgccattga ccacgttcac
    22681 cacgcccggc ggcagtaaat caccgacaat ttccattagc agcagtacag aaagcggggt
    22741 aagacgtgcg ggtttcagca ccacacagtt gcccgccgcc agcgcgggag ccattttcca
    22801 gctcgccatc agcagcggga agttccacgg gataatctgc cccaccacgc ctaacggttc
    22861 atggaaatga taggccacgg tttcgctatc aacttcactg atcccacctt cctgcgcccg
    22921 aatacacgag gcgaaatagc gaaaatggtc aatcgccagc ggcacgtcgg cagcactggt
    22981 ttcgcgaatg ggtttgccgt tatcccaggt ttcagccgtc gctaacagct caaggttttg
    23041 ttccattcga tcggcaatct taaacagaat cgccgcgcga tcctgcaccg aggtgtgcgc
    23101 ccatttatct ttcactttgt gcgcagcatc cagcgccaga tcgatgtctc gtttgcccga
    23161 agacgccact tcgcacagca gctgcccggt caccggcgtc agattctggt aatactcgcc
    23221 gtcggcaggg gctacccatt cgccgccaat aaagttgtca tagcgggctt ttaacttgag
    23281 ggggaaacca tactcgccgg gcttaatctg tgctgaaggg ggattattgg tcatgatcgt
    23341 ctccttgcgg tgtgaggtat aacaagggta gacgtgactg gcgaaatctt cgccagtcgg
    23401 taacagcttt acgacagcta tcacgaattt acgggcaagg gttacagaga tagttgaaaa
    23461 agcggctgac aatttgccag ccgttgtgga aatgatgatt acatcgctgc gcgataaatc
    23521 gccacaattt cttcgtgagt tgcctggatc gggttagtaa aaccacaggc atctttcagg
    23581 gcattagtcg ccagaaccgc gaaatcttct tctttcacgt tcaggtcgcg taggcctgcc
    23641 gggatatcca ctttcttcgc cagttcacgg atggcgttaa tgcaggcttc agcaccttcc
    23701 gcatcgtttt tacctgtcac gttcacgccc attgcagcgg cacagtcacg cagacgtgcg
    23761 gcggcgactt tgctgttgaa tacctgaaca tgcggcagca aaacggcgtt acatacaccg
    23821 tgtggcaggt tgtagaaacc gcccagctgg tgcgccatcg catgaacata acccagagaa
    23881 gcattattga acgccatacc agcaaggaac tgggcataag ccattgcttc acgcgctttc
    23941 gcattactgc catcttcaac ggctaacggc aggttttcgg caatcatggt cacggctttc
    24001 agtgcacaag cgtcagtgat cggcgtggcg gcaatagaaa catatgcttc gatagcgtgc
    24061 gttaaggcat ccatacccgt tgcggcggtc agtgacttcg gcataccaat catcagagag
    24121 gagtcattga cagaaagcag cggagtgaca tgtttatcaa caatcgccat tttgatatga
    24181 cgcgcttcgt cagtgatgat gcagaaacgg gtcatttcag aggccgtacc cgcagtggtg
    24241 ttgatggcga tcatcggcag ctgcggtttt gcagagcggt caacgccttc gtaatcgcga
    24301 atatcgccgc cattggctgc caccagcgca ataccttttg cgcagtcgtg tggagaacca
    24361 ccgcctaagg agatcacgct atcgcaatta ttttctttaa gtaatttcaa acctgcggcg
    24421 acgttttccg tggtggggtt aggttgggtg ccatcataaa taacgctaaa aatattgcgt
    24481 tcttccagtg ctttttgcac atcacccgcc atacctaatt tcgttaacat actgtcagtg
    24541 acaattaagg tacgggtaaa tccataatct gccatcatat tcattgcatc agtcaatgaa
    24601 tcagcgccga tgacattcac agaaggaata aagaaagttg aagatgccat aacacactcc
    24661 tgaaagtgta aaaaagggtg ggcagcttac tgaggatttt cattcgaaaa catgatcatt
    24721 attattgatt atcaaaatta atctaataaa aagcacattg tattcattaa acaatgtgct
    24781 ttttttaaaa tcataagaaa tttgtattaa tttattttaa aaattaatta atcatttcct
    24841 tatttttccg gaaataataa tgcgtcgcgc aataaatgat catttccacg acgacgcgta
    24901 aagccaatgc ggtcaaaata ttccagaatc tgaattgcca gctttcggcc tacgcctaag
    24961 cgatcgcgga aatccgccgc gcaggttgaa ccacactcct gatcgagatc gcggatcata
    25021 ttggcaaatt cgacaatccg atcgttacgg taataacgat ctttgacgat cgcggtaatt
    25081 attccttgct gcgccgcctg gcgtagcgtc aggcgcattg cctgctcgtc ggtacccgtc
    25141 tcttttgcca gatcacgcac ccaccacggc tcgtcaccaa acagcggctc tgctttttgc
    25201 cagatggcct gctgctcttc gctgaagccc gctttgtgat ccggcagatg cagccaaccg
    25261 tgatgactga ggatgtcgcc gctttcgcgc atcttttcaa tcagcaacag tactagcgct
    25321 tcatcttcca ttggcaacgc catacgtcgc agacgttcgc gcccaggccc aggttcatcg
    25381 cgatgttgct catgataagt cgctaatgtg tcgagaattt tccgttgcca gcgagcggca
    25441 accggcgcat tcaacaagct ataaccagcc tgaatatagc caggctgttg cagcaattcg
    25501 cgcatccctt cgccgttgag ctggcgcgcc caggcgaaat ccgcaaggtt aaccgcgccg
    25561 cgttccagat gaacagataa cgcatcggca tcgctctgcg cccgtgcaag agacgccagc
    25621 cattgcagat attccggctt acgtttaccg cgacgcggcg ggttaagcat caccacacgc
    25681 gcccccgcca gcgtgttgcg ggccgagata tcgcgcagca ctaagcgatc gttatccgcc
    25741 agccacagcg gcgtgtcgaa caccagctcg gcaaggttat cttccagcag cgaaacgcgt
    25801 cccgtaacgt ggctggcggc gtggtgaata tgcagcggct gccactgggt cagcggcgta
    25861 tgggtttgaa gctcgacaat cacccgtgtg aacggctctg gcggcgcatc ggcaagcagc
    25921 cagtcgccac ggttaatctg ctctttttcc gcatcacccg cgatgttaag cgcgatacgc
    25981 tgcccggcat gggcggtttc tgttggctgg ttttgtgcat gcagcgcacg tacacgcatc
    26041 ggtttattta caccagtcag ccagagtgaa tcgcctacct tcacttcccc gcttaacgcc
    26101 gtaccggtga cgaccagccc ggcacctttt acggtaaatg cgcggtcgat cgcgaggcgg
    26161 aaactatgtt ggctggcgtg ttcacgtcct ggtaactgaa gcagatgctc gcgcagggca
    26221 tcaattcccc gaccttcggt tgctgcggtg ataaacagtt ttgcctcagc aaaaccgtat
    26281 tcccgcagca cctcctttac ctggcgttca acctcatcaa cacgcgcttc gtccacgcga
    26341 tcggccttgg tcagcgccac tgtcagcatc gggttaccgg tcagctgcaa aatcgccaga
    26401 tgctcacggg tctgcgccat cacgccgtca tcgcacgcca ccaccaacag cgcgtgatcg
    26461 ataccaccaa cgcccgccag catgttggaa agaaactttt catgaccggg tacgtcgata
    26521 aaaccaggca cgcgaccatc cggctgcggc cagtaggcat agccgagatc gatggtcatg
    26581 ccgcgctttt tttcttccgg cagacggtca gcatttacgc cagtaatcgc ctgcaataag
    26641 gttgttttgc cgtggtcaac gtgtccggca gtcgcaataa tcatttcaac aacatctcca
    26701 aaaaccgttg ctcatcttca aggcagcgta aatccagcca caatcgtccg tcataaatac
    26761 gaccaatcac cggcactggc aattcacgcc agcgggcggc taatgactca aggtggctac
    26821 cgcgtccatc atggggtgta aacgttaatg ccgcgctcgg caggcgatca accggcagcg
    26881 aaccactgcc aatctgcgaa agacatggca taacctgtac cgcaaactcc gcgccgtaat
    26941 gtgcggcaag gggagcctgt aaacgttgtg cctggatttg aatgacctct gcgctgcggg
    27001 taagcaggcg cagggtcggt aatttttcac tcagagcttc agggtgtaaa taaagacgca
    27061 acgtggcttc cagcgccgcg agggtcattt tatccgcgcg taatgcacgc ttcagcgggt
    27121 ggctttgcag gcgggcgatc atctcttttt taccaacaat aattcctgcc tgcggcccgc
    27181 ctaacaactt gtcgccggag aaactcacca gactgacgcc cgccgcaatc aactcctgcg
    27241 gcattggctc tttcggcaaa ccgtactggc taagatcgac cagcgagcca ctgcctaaat
    27301 cagtcactac gggaacatcc agctctttgc cgagcgccac cagttccgct tcatctatcg
    27361 ctttggtgaa cccctgaatg ctgtagttac tggtatgtac tttcatcaac agtgcggtat
    27421 tttcattcac cgcctgacga taatcattcg cgtgcgtgcg gttggtggtc cctacttcgt
    27481 gtagggtgca acctgcctga cgcataacat cgggaatacg aaacgcgccg ccaatctcca
    27541 ccaattcgcc gcgagatact accacctctt tcccgctggc agtggccgcc aacatcaata
    27601 acaccgccgc cgcattgtta ttgacgatac aggcatcttc cgcccccgta atacggcaca
    27661 gcagttgcgc cagcgcccga tcgcgatgtc cgcgtccggc gtcgtccaga tcgtactcga
    27721 gggtcactgg cgaacgcata gcctgcgcaa cggcttccac cgcggcttcc gcctgtaaag
    27781 ctcgtccaag gttggtatgc agcacggttc ccgtcaggtt gatcaccgga cgcagcgcgc
    27841 tctgcgcttc tttcgtcaac cgggcatcga cttcttgcgc ccagttttca caccacgcag
    27901 gcagcgtctg gctgccacga atcacttctc gcgcttcgtc gagcatctga cgcaacaatt
    27961 ccaccacgcg ggtgtgacca taagtatcac gcaaagaaag gaaggagcta tcgcgcaata
    28021 agcgatcaat agccggaagt tgactataga gggaacgcgt ttcggttgtc ataggaaacc
    28081 tggctgatca aggccctctc acacggagaa gggcgtttaa cataaccacg gattgtaacg
    28141 tgagatgggt caggaggaca tatcgcgcat caagcctttg gcggttcggt gcgagcaaaa
    28201 ctttcgcggc taaacaggtt ttcgaccagt ttgactaagt gtgggcggtc gacacaccaa
    28261 cctggcgcaa cgcggcggaa attgagatat ccgacggcac aggcaatggc gatagtcgcc
    28321 agattgaccg tatcggtttt gagtgtgcca tcgaccagat atccttccag cacatccaga
    28381 ctgcggttga ttttctcccg ctggcgtaac aattcatctt cagactgctg cgccgctgga
    28441 cgcgcctgtt cacgcaccga taccagcccg gcatccataa tgccatccgc cagtgcctca
    28501 attttgcgca cccgcaacga ctccagcgga tcgcgcggca acatcgccgg agcgacattc
    28561 attaattcaa tatattcagc gatgatcggc gaatcaaacc aacattcgcc ctcttcggtc
    28621 accagcaccg gcacttttcc taacgggtta aattgcgcca cgccgttgtc cgcgttatag
    28681 ggcagttcat taataaattc gaaagttatg cccttttcta acaacagaat agaaagtttg
    28741 cgtacaaacg ggctggtgta gctaccgacg agtttcatgc cgagtccttt gtgcgaggaa
    28801 aaatatcagt atggcctggt gatgggaaaa agggcagaaa atgttgatgg tttgttactt
    28861 ccaaataaat cacatattta tcatggtgat ataaatattt tcctaattgt ttctatctga
    28921 t
//
LOCUS       DAMZET010000041        28768 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.041, whole genome shotgun sequence.
ACCESSION   DAMZET010000041 DAMZET010000000
VERSION     DAMZET010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.041"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1062)
                     /locus_tag="POB34_001896"
     CDS             complement(<1..1062)
                     /locus_tag="POB34_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HDI5918857.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLS"
     gene            1162..1980
                     /gene="cof"
                     /locus_tag="POB34_001897"
     CDS             1162..1980
                     /gene="cof"
                     /locus_tag="POB34_001897"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HDI5918858.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            2133..2591
                     /gene="decR"
                     /locus_tag="POB34_001898"
     CDS             2133..2591
                     /gene="decR"
                     /locus_tag="POB34_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HDI5918859.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            2621..4393
                     /locus_tag="POB34_001899"
     CDS             2621..4393
                     /locus_tag="POB34_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDI5918860.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            4386..6167
                     /locus_tag="POB34_001900"
     CDS             4386..6167
                     /locus_tag="POB34_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDI5918861.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            6348..6686
                     /gene="glnK"
                     /locus_tag="POB34_001901"
     CDS             6348..6686
                     /gene="glnK"
                     /locus_tag="POB34_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDI5918862.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            6716..8002
                     /gene="amtB"
                     /locus_tag="POB34_001902"
     CDS             6716..8002
                     /gene="amtB"
                     /locus_tag="POB34_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HDI5918863.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(8051..8911)
                     /gene="tesB"
                     /locus_tag="POB34_001903"
     CDS             complement(8051..8911)
                     /gene="tesB"
                     /locus_tag="POB34_001903"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HDI5918864.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            9129..9701
                     /locus_tag="POB34_001904"
     CDS             9129..9701
                     /locus_tag="POB34_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HDI5918865.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(9732..10043)
                     /locus_tag="POB34_001905"
     CDS             complement(9732..10043)
                     /locus_tag="POB34_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HDI5918866.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            10206..10302
                     /gene="ffs"
                     /locus_tag="POB34_001906"
     ncRNA           10206..10302
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="POB34_001906"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            10422..10775
                     /locus_tag="POB34_001907"
                     /pseudo
     CDS             10422..10775
                     /locus_tag="POB34_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
     gene            complement(10817..12367)
                     /gene="pdeB"
                     /locus_tag="POB34_001908"
     CDS             complement(10817..12367)
                     /gene="pdeB"
                     /locus_tag="POB34_001908"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HDI5918867.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(12531..13001)
                     /locus_tag="POB34_001909"
     CDS             complement(12531..13001)
                     /locus_tag="POB34_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HDI5918868.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(13117..13668)
                     /gene="maa"
                     /locus_tag="POB34_001910"
     CDS             complement(13117..13668)
                     /gene="maa"
                     /locus_tag="POB34_001910"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HDI5918869.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPNNVVVGGNPARIIKKL"
     gene            complement(13840..14058)
                     /gene="hha"
                     /locus_tag="POB34_001911"
     CDS             complement(13840..14058)
                     /gene="hha"
                     /locus_tag="POB34_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HDI5918870.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(14084..14458)
                     /gene="tomB"
                     /locus_tag="POB34_001912"
     CDS             complement(14084..14458)
                     /gene="tomB"
                     /locus_tag="POB34_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HDI5918871.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(15004..18153)
                     /locus_tag="POB34_001913"
     CDS             complement(15004..18153)
                     /locus_tag="POB34_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDI5918872.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(18176..19369)
                     /gene="acrA"
                     /locus_tag="POB34_001914"
     CDS             complement(18176..19369)
                     /gene="acrA"
                     /locus_tag="POB34_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HDI5918873.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            19511..20158
                     /gene="acrR"
                     /locus_tag="POB34_001915"
     CDS             19511..20158
                     /gene="acrR"
                     /locus_tag="POB34_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HDI5918874.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            20286..23648
                     /gene="mscK"
                     /locus_tag="POB34_001916"
     CDS             20286..23648
                     /gene="mscK"
                     /locus_tag="POB34_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HDI5918875.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(23860..24021)
                     /gene="rsmS"
                     /locus_tag="POB34_001917"
     CDS             complement(23860..24021)
                     /gene="rsmS"
                     /locus_tag="POB34_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HDI5918876.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(24035..24562)
                     /gene="priC"
                     /locus_tag="POB34_001918"
     CDS             complement(24035..24562)
                     /gene="priC"
                     /locus_tag="POB34_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HDI5918877.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            24632..25009
                     /locus_tag="POB34_001919"
     CDS             24632..25009
                     /locus_tag="POB34_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HDI5918878.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            25162..25713
                     /gene="apt"
                     /locus_tag="POB34_001920"
     CDS             25162..25713
                     /gene="apt"
                     /locus_tag="POB34_001920"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDI5918879.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            25842..27773
                     /gene="dnaX"
                     /locus_tag="POB34_001921"
     CDS             25842..27773
                     /gene="dnaX"
                     /locus_tag="POB34_001921"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDI5918880.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    27106..27170
                     /gene="dnaX"
                     /locus_tag="POB34_001921"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            27826..28155
                     /locus_tag="POB34_001922"
     CDS             27826..28155
                     /locus_tag="POB34_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDI5918881.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            28155..28760
                     /gene="recR"
                     /locus_tag="POB34_001923"
     CDS             28155..28760
                     /gene="recR"
                     /locus_tag="POB34_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDI5918882.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
BASE COUNT         6823 a         7270 c         7690 g         6985 t
ORIGIN      
        1 ggagagtcgg ggacttttgc gcaacgttaa atagcaaaaa cctaaactga tgccactact
       61 gacctggctg acccgttgca gctcctccgg tttgccgatg gtgatttgca cgggatgccg
      121 acaactggtt cccaaatctt tttcgaaaag cggcggagtt atccagtact caaccgcttt
      181 aagcagcgga tgacgcaaat ggtaataatc atggctctcc aggcgcacca gctccgctgt
      241 gaattgtgtt aagcgaaaag gaccagtgcc gatcagtggg aattgcggat gcgccagatg
      301 gctgcaatag ctcgccagcc ggtgcgcaag ccagtaatcg gggcgatgta aaaagaaggt
      361 cagacactgc ggatgggtga cttcaatacg cttcacgcta ataaataatt gatccagtgc
      421 tggcagttgt aacagcatca ataatcgctg gtgtaagtgt gaggctttta ctgcatcgcc
      481 gttatgccag tgtagggttg aacgaagata aaagtcccag cgtaacccgt cagtagaggt
      541 ttcccagtga tgcgctaaat cgccaatcgg gcgctgagta ttattatcga agcgggtcag
      601 gccggaaaat atctgcccgg cgagatgctg ctcggcacgg ccgggcaaaa agcctggttg
      661 tagcggttcg agcgggcgat agtagggaat acgcaacgtg ggtgtatcgt tttgccattg
      721 tccgcccata aacggctgta acagagtgcg cagctcacct ggggccagtt gcgccagctc
      781 cagcacatct tgctgctttc cggtttccag tgcctgttcc atcatcgcat tgcgtagcga
      841 ttccggcgtg accagaaagc gtaattgtcc gcgctttccg cgtcctgact gcgcctgcca
      901 ctccagccat cccgcctcct gtgcctgacg caacagcgta cgaacatggc gttcgctgca
      961 aaaacagcgt tcggccagtt cgctgacggt gacggtttgc ggctttccgg ctgaaggttg
     1021 ccacagacgt tgatactggt taagacggtt gagcaatcgc atataaaccc ggaacaatat
     1081 tatttaacta ttcactatta cttccgtata tatcaggtga tactcaatca ccattaaccg
     1141 tgtcacagag tggagaaaga aatggctcgt ctggcagcgt ttgatatgga tggcacttta
     1201 ttgatgcctg accatcattt aggtgagaaa accctctcta ctttggcgcg actgcgtgaa
     1261 cgcgacatta ccctcacttt tgccacgggg cgtcatgcgc tggagatgca gcatattctc
     1321 ggggcgctat cgctggatgc atatttgatt accggcaacg gaacgcgcgt gcattctctg
     1381 gaaggtgaac ttttacatcg tgatgattta cctgcggatg tcgcggagct ggtgctgtat
     1441 cagcaatggg atacccgagc cagcatgcat atcttcaatg acgacggttg gtttaccggg
     1501 aaagagatcc ctgcgttgtt gcaggcattt gtctatagcg gttttcgtta tcagataatt
     1561 gatgtcaaaa aaatgccact cggcagcgtc accaagatct gcttctgtgg cgatcacgac
     1621 gatcttacac gcttgcagat ccagctatac gaagcattag gcgagcgtgc acatttgtgt
     1681 ttttccgcca cggattgcct cgaagtgctg ccggtgggct gcaataaagg cgctgcattg
     1741 acggtgctga cccaacattt aggtttatcg ttgcgcgatt gcatggcctt tggtgatgcg
     1801 atgaacgatc gcgaaatgtt aggcagcgtc ggtagcggct ttattatggg caatgcgatg
     1861 ccgcaactgc gcgcggagct cccgcattta ccggtgattg gacattgccg aaatcaggct
     1921 gtctctcact atttgacgca ctggctggac tatccacatc taccttattc ccccgaataa
     1981 cgagatccct tccagcaccg ggcaattgcc cggttttttt tgcgttgaat ttgtcatttt
     2041 gtgccgtggt gtttaaaccg cacagaataa attgtcgcga tttcaccttt aaaatagaat
     2101 taaaagagaa aaaaattctc cgtggaaggg ctatgttaga taaaattgac cgtaagctgc
     2161 tggccttact gcagcaggat tgcaccctct ctttgcaggc actggctgaa gccgttaatc
     2221 tgacaaccac cccttgctgg aagcgcctga aacggctgga ggacgacggt atccttatcg
     2281 gcaaagtcgc cctgctggat ccggaaaaaa taggcctcgg cctgaccgcc tttgtgctga
     2341 taaaaacgca acatcacagc agcgaatggt attgccgctt tgtcacggtg gttaccgaaa
     2401 tgccagaagt gctggggttc tggcgcatgg ctggtgaata cgattatctg atgcgcgtcc
     2461 aggttgccga catgaaacgc tacgacgagt tttataagcg tctggtaaac agcgtgccgg
     2521 ggctgtcgga cgtcacttcc agcttcgcga tggaacagat taaatacacc acttctttac
     2581 ccatcgaata aatatccaga atcaggtcag gacacaacgc gtgcgattat ttgctcaatt
     2641 aagctggtat ttccgtcggg aatggcgtcg ctatctcggg gctgtcgcct tgcttgtcat
     2701 tatcgcgatg ctgcaactgg ttccgccaaa agtggttggt attgttgtcg atggcgtgac
     2761 agaacaacac tttactaccg ggcagatcct gatgtggatc gccaccatgg tgctgattgc
     2821 cgttgtggtt tatctcctgc gttacgtctg gcgggtattg ctgtttggtg cgtcttatca
     2881 actggctgtt gaactgcgtg aagattatta ccgtcagcta agccggcagc atcctgagtt
     2941 ttacctgcgt catcgcacgg gtgacctcat ggctcgtgcg actaatgacg tcgatcgcgt
     3001 cgtgtttgcc gccggagaag gggtgctgac gctggtggat tcactggtaa tgggctgcgc
     3061 tgtgttgatt atgatgtcta cgcaaattag ctggcagttg accttatttg ccctgttacc
     3121 gatgccagtg atggcgatca tgattaagcg caacggcgat gccttgcatg aacgctttaa
     3181 gctggcacag gcggcgtttt ctagtcttaa tgaccgcacc caggaaagcc tcaccagtat
     3241 tcgcatgatc aaagcctttg gtctggaaga tcgccagtcg gcgttatttg ccgcggatgc
     3301 cgaagatacc ggcaaaaaaa acatgcgggt ggcgcgtatt gatgctcgtt tcgacccgac
     3361 catctatatc gcgattggta tggcgaactt gctggcgatt ggcggcggta gttggatggt
     3421 ggtgcagggc agtttaacgc tgggccagct caccagtttt atgatgtatt taggtctgat
     3481 gatttggcca atgctggcgc tggcatggat gtttaacatt gtggaacgtg gtagtgctgc
     3541 gtacagccgt attcgcgcga tgctggcgga agcgccggtg gtgaacgatg gtagcgaacc
     3601 cgtgccggaa gggcgtggcg aactggatgt aaatattcac cagttcacgt atccgcagac
     3661 tgaccatcct gcgctggaaa acgtcaattt cgccctgaaa cccggtcaga tgctgggtat
     3721 ttgcgggccg actggttccg gcaaaagtac cctgttgtcg ctcattcagc gtcatttcga
     3781 cgtcagcgag ggggatattc gctttcatga tattcctctg acgaagttac aactcgatag
     3841 ctggcgtagc cgcctggcgg tggtcagcca gacgccattc cttttttctg acaccgtggc
     3901 gaataacatc gcgctgggtt gcccgaatgc cacccagcaa gagattgaac acgtagcgcg
     3961 gttagccagc gtacatgacg atattttgcg tctgccgcaa ggttacgata cagaagtggg
     4021 cgagcgcggc gtgatgcttt ccggtgggca aaaacagcgt atctccattg ctcgagcgtt
     4081 attagtcaac gcggaaatcc tcatccttga tgatgcgctt tcggcggtgg acggacgcac
     4141 agagcaccag atcctgcata acctgcgtca gtggggacag ggaagaacgg taatcatcag
     4201 tgcccatcgc ctttcggcac tgacggaagc cagtgaaatt attgtgatgc agcacggaca
     4261 tatcgcccag cgtggcaatc atgatgagct ggcgcaacaa agcggctggt atcgcgatat
     4321 gtatcgctat caacaactgg aggcggcgct cgacgacgtt ccggaaattc gcgaggaggc
     4381 cgtcgatgcg tagttttagc caactgtggc cgactctcaa gcgtctgtta gcgtacggtt
     4441 cgccgtggcg gaaaccgctg gggattgcgg tcctgatgat gtgggttgcg gcagcggcag
     4501 aagtcagtgg gccgctgctt atcagctatt ttatcgacaa tatggtagcg aaaaataacc
     4561 tgccgttgaa aatggttgca gggctggcgg cggcgtatgt tgggctacaa ctgtttgccg
     4621 ccgggctaca ttacgcgcag tcgctgctgt ttaatcgggc ggcagtaggc gtagtgcaac
     4681 agttgcgtac cgacgtgatg gatgctgcgt tacgccagcc attaagcgag tttgataccc
     4741 aacccgtcgg gcaggtgatt tcccgcgtca ctaatgacac tgaagtgatc cgcgatctct
     4801 acgttaccgt agtggcaact gtcctgcgta gcgccgcact ggtgggcgcg atgctggtgg
     4861 caatgttcag cctcgactgg cgaatggcgc tggtggcgat aatgattttc ccggtagtgc
     4921 tggtggtaat ggtgatatat caacgttaca gcacgccgat tgtccgtcgt gtgcgcgcct
     4981 atctggcgga tatcaacgac ggctttaacg aaatcatcaa tggcatgagc gttatccagc
     5041 agttccgtca gcaggcgcga tttggcgaac gtatgggtga ggccagtcgt tcacactata
     5101 tggcgaggat gcaaaccctg cgcctcgacg gttttctgct gcgtccgctg ctgagtctgt
     5161 tttcatcgct cattctttgt ggcttgttga tgctgtttgg cttctccgcc agcggcacca
     5221 ttgaagtggg cgtgttatat gcgtttatca gctatcttgg gcgacttaac gaacccttaa
     5281 tcgaactgac cacgcagcag gcgatgctgc aacaggctgt tgttgctggt gagcgcgtgt
     5341 ttgaactgat ggacggaccg cgccagcaat atggcaatga tgatcgcccg ttacagagcg
     5401 gcaccatcga agtcgataac gtgtcatttg cttatcgcga tgacaatctg gtgctaaaga
     5461 acattaatct ctctgtgcct tcgcgtaact ttgtggcgct ggtcgggcat accggcagtg
     5521 gcaaaagcac cctcgccagt ttattgatgg gctattaccc gctaacggaa ggtgagattc
     5581 gccttgatgg tcgtccatta agttcgctaa gtcacagcgc gctgcgccag ggcgtggcaa
     5641 tggtgcagca agatccggtg gtgctggcgg ataccttcct cgccaacgtg acgctgggac
     5701 gtgatatctc cgaagaacgc gtctggcagg cgctggaaac cgtgcaactg gcggagctgg
     5761 cgcgcagcat gagtgatggt atttatacgc cgctgggcga gcagggaaat aatctctcag
     5821 tcgggcaaaa gcaactgctg gcactggcgc gcgtgctggt ggagacgccg caaatcctga
     5881 tccttgatga ggcaaccgcc agcattgact ccggtactga acaggcgatt caacatgctc
     5941 tggcggcggt gcgtgaacat accacgctgg tggtgattgc tcaccgctta tcaactattg
     6001 ttgatgccga caccattctg gtgcttcatc gtggacaagc cgtggagcag ggcactcacc
     6061 agcaactgct ggcggcccag ggacgctact ggcagatgta tcaactgcaa cttgcgggcg
     6121 aagagctggc agccagcgtg cgtgaagagg aatcattgag cgcctgaata gcgcaatatt
     6181 tcatcgttgg tgcaaaaatg taacgcactg tgcactgtca tagtgcgttt tcattttcaa
     6241 tcttcttaac ttcctgctct ctttctcatt tttcatttct ggcacaccgc ttgcaatatc
     6301 ttcttcgtgt agcagaacca ttactgaatt ctgaccggag gggatctatg aagctggtga
     6361 ccgtgataat caaaccattc aagctggaag acgttcgtga agcgttatct tccattggta
     6421 ttcagggcct gaccgtcacc gaagtgaaag gtttcgggcg tcagaaaggg catgccgagc
     6481 tgtaccgggg ggcggaatac agcgtcaact tcctgccaaa agtaaaaatt gatgtggcga
     6541 ttgctgatga ccaactcgat gaagtgatcg atatcgtcag taaggcggct tacaccggaa
     6601 aaattggcga cggcaaaatc ttcgtcgctg aattgcaacg cgtcattcgt attcgtaccg
     6661 gcgaagccga cgaagcggcg ctgtaatctc tggcacacag caacaggaac gaaaaatgaa
     6721 gatagcgacg ataaaaactg ggcttgcttc actggcgatg ctgccgggac tggtaatggc
     6781 tgcacctgcg gtggccgata aagccgacaa tgcgtttatg atgatttgta ctgcgctggt
     6841 gctgtttatg actattccgg ggattgccct gttttacggt gggttgattc gcggcaaaaa
     6901 cgtgctgtcg atgctgacgc aggtgacggt gacgtttgca ctggtctgta ttctctgggt
     6961 ggtttacggt tactcgctgg cgtttggtga gggcaacaac ttcttcggca acattaactg
     7021 gttgatgcta aaaaacatcg aactgacggc ggtgatgggc agcatttatc agtatatcca
     7081 cgtggcgttt cagggctcgt ttgcctgcat taccgtcggc ttgatcgttg gggcgctggc
     7141 ggaacgaatc cgcttctcag ctgtgttgat tttcgtggtg gtatggctga cgctctctta
     7201 cattccgatt gcgcatatgg tgtggggcgg tggtttgctg gcttctcacg gtgcgctgga
     7261 tttcgcgggt ggcacggtgg tgcatattaa cgccgcaatc gccggtctgg tgggcgcgta
     7321 tctgataggc aaacgcgtgg gcttcggcaa agaggcgttt aaaccgcaca acctgccaat
     7381 ggtcttcacc ggtactgcca ttctctatat tggttggttt ggctttaacg ccgggtcagc
     7441 gggcacggcg aatgaaatcg cggcactggc atttgtgaat actgtggtcg caacggcggc
     7501 ggcaattctt ggctggatct tcggtgaatg ggcgctgcgt ggtaagcctt ctctgctggg
     7561 agcgtgttct ggcgcgattg ccggtctggt cggcgtgacg ccagcgtgtg gctacattgg
     7621 ggttggcggc gcgttgatta tcggcgtggt agctggtctg gcgggcttgt ggggcgttac
     7681 tatgctcaaa cgcttgctgc gggtggatga tccctgcgat gtcttcggtg tgcacggcgt
     7741 ttgtggcatt gtcggctgta tcatgactgg gatttttgcc gccagttcgc tgggtggcgt
     7801 gggcttcgct gaaggtgtga cgatgggcca tcagttgctg gtacagctgg aaagcatcgc
     7861 cattacgatc gtctggtccg gtgtggtggc atttattggc tacaaactgg cggatctgac
     7921 ggttggccta cgtgtgccgg aagaacaaga gcgagaaggg ctggacgtca acagccacgg
     7981 cgagaatgcc tataacgcgt aacaagcact gcaaaaaata gccggacggt tttcacctcc
     8041 ggctattttt ttaattgtga ttacgcatta ccccttcctg aacggtcgag gcaaccagca
     8101 cgccgtcctg ggtataaaac tcaccgcgca caaagccgcg tgcgctggac gccgaggtgc
     8161 tctccacgct atacagcagc cattcattca aattaaacgg gcgatggaac cacatggaat
     8221 ggtcaatggt ggcaatctga atccctggtt cgagaaaacc gatgccgtgc ggctgtagag
     8281 ctaccggcag gaagttaaga tcagaagcgt aaccgagcag atactgatga acgcgcaggt
     8341 catccggcac gctaccattt gcgcggatcc acacctgacg atgtggttct gcgacgtgac
     8401 ctttcagtgg gttatgaaac tccaccggac ggacttccag cggacgatcg cagatgaatt
     8461 tatctttaag cactggcggc agcaggtgcg ccagcgattg ggcgatttgc gtttccgaag
     8521 gcaggccatc aggcgctggc gcggacggca ttgttttttg atgttcgaaa cccgcttctg
     8581 gtgcctggaa agaggccgtc atataaaaaa tcggtttgcc gttttgaata gcagcaaccc
     8641 ggcgggcgct gaagctgtta ccgtcacgca gcgtttcgac atcataaata atcggcttct
     8701 tactatcgcc agggcgaaga aagtagctgt gaaacgaatg taccagccgc tcttcaggga
     8761 cggtctcttt tgcagcatac aaggcctgac ccacgacctg gccgccaaac acctggcgta
     8821 aacctaaatc ttcactctgg ccgcgaaaga gtccttcctc aattttttct agatttaaca
     8881 atgtcagtaa attttttagc gcctgactca tataactctc cagtaacaaa gctgccgcag
     8941 caagccaaag tgagttgagt ataacgcaaa tttgccactg gtccgatggg tgcaatggtc
     9001 tgaattacgg gctaattaca ggcagaaatg cgtgatgtgt gccacacttg ttgacgttac
     9061 tattttgtta accactcttc cggcgaggaa agttagcccg ctggtgcatt gataataagg
     9121 agaaatgaat gaaactcgtg cacatggcca gtggtttagc ggttgcgatt gcattggcgg
     9181 cttgcgcaga taaaagcgcg gatattcaga cgccagcccc ggctgcaaat acgtctattt
     9241 cagcaacaca acaacctgct atccagcaac cgaatgtctc cggtaccgtc tggatccgtc
     9301 agaaagtcgc actgccgcct gatgctgtgc tgaccgtgac actttctgac gcgtcgttag
     9361 ccgatgcacc gtcaaaagtg ctggcgcaga aagcggtgcg taccgaaggt aaacagtcac
     9421 cattcagctt tgttctgcca tttaacccgg cagatgttca gccgaacgcg cgtattctgt
     9481 tgagtgcggc gattaccgtg aatgacaaac tggtatttat caccgatacc gttcagccgg
     9541 tgatcaacca gggcggaact aaagccgacc tgacattggt gccggtacag caaaccgccg
     9601 tgccggttca ggccagcggt ggcgcaacga ctaccgtacc ttcgacttca ccaactcagg
     9661 tgaatccgtc ttcggcagtt ccagctccta cgcaatatta agcgacgtta accctctccg
     9721 cccggagagg gttagtagtt ccagcgataa cgctgcaagt cgatttgccc gcttcccgat
     9781 accattacac cttctgccag taatgcctgt cgctgacgct gtaaatccgg tccggttagc
     9841 gaaattgtgc cgtggcgatt aaccacccgg tgccagggta aggtgctgcc ttcagggaga
     9901 cgctttaaca caccgcccac ctggcgcgcg gcgcggggcg atcccgccag tttcgccaca
     9961 tcaccgtaag tggtgacata gccttcggga atagcggcga cgatttgcca gacgcgttgg
    10021 ggaaatgaat cttctttttc catctttttt tcctgaggta atttttcagc ataatctgga
    10081 aaaacgcccg agtgaagtcg cattgcgcaa gaaaccagca tctggcacgc gatgggttgc
    10141 aattagccgg ggcagcagtg ataatgcgcc tgcgcgttgg ttctcaacgc tctcaatggg
    10201 ggctctgttg gttctcccgc aacgctactc tgtttaccag gtcaggtccg gaaggaagca
    10261 gccaaggcag atgacgcgtg tgccgggatg tagctggcag ggcccccacc catttctgcc
    10321 tcccaccgtt tcgtcaaaaa accccaacat ggctaaactt taaccacaac tgacgtcgca
    10381 agaattgtct ggctgcgcag tacgcttcgg aggtatgtct gatgaagtat gttgatggtt
    10441 ttgtggttgc cgttcctgcc gataaaaagg atgcctagcg ggaaatggcc gctaaggctg
    10501 cgccattgtt taaagagttt ggcgcgcttc gtattgtcga atgctgggcc agcgatgtac
    10561 cggatggcaa agtgaccgat tttcgtatgg cggtgaaagc ggaagagaat gaagaggttg
    10621 tctttagctg gattgaatac ccttcaaaag aggtccgcga cgctgctaat caaaagatga
    10681 tgtcggaccc acggatgaaa gagttcggcg agtccatgcc gtttgacggc aagcgaatga
    10741 tctatggcgg attcgagtca atcatcgacg aataggtagc gtgacgggct gtgcggcgtg
    10801 ccgtcagccc aaaaattcac aaatgttgct cggcccagcg taagaaatct tctttcggta
    10861 atgccttgct gtagagccag ccctgaccgt agtgcacgcc atgctggcgt agccactctt
    10921 cttgtttact ggtttcgatt ccctccgcta ccattttcag tttcagtgtt tttgccattt
    10981 cgatgatatg cggcgtgaca tttttatatt ccagcgcatc aacgaaagat ttatcgatct
    11041 tcagaatgtc gacatccaga tcctgtaaat aacttaaact tgaatatccc gtaccaaaat
    11101 catcaagata aatttcatgg cccgcctccc ggtagcgaga aattatcggg gcgctggttt
    11161 tcggatcggc aaactcgcgt tcagtgagtt caagcgcgat ctgtctggga ttaacctgat
    11221 agtgattgat catttcacgc agcaattgcg ggattttttc cgaggtgagc acggtggatt
    11281 caagattgat cgaaatatgc tgctgtggat gctgacgcag ccagtcgccc atatcttcaa
    11341 agacgcttct tataatcagt agcgtcaatg gctcagaaag gcccgtttgc tgtgccagcg
    11401 gaataaaact atctggtgac aaccaactac cgtctgtctg cggccagcgc gccagtgcct
    11461 cagcaccgac aattttgcca ttggctaagg agacaatcgg ctgatagtgc acgcaaatat
    11521 cacgattttc gatagcatcc tgcagccgat gatgcggtga ctggatacgg cgcaaaatac
    11581 gcagcacaaa catcgctgcc agcaggccaa tcaccaaccc gagcggtaac caaataaaga
    11641 cttgccgatg ccagcctttc tgcaacattt tcgttgaagc ccaggtgatg atcgaaatat
    11701 tcatctccgg gaagggcagg atatcgtaaa tgattccatt attttcgata tgctcaccgg
    11761 gtgttttttg tagcctggta ataattccct gagcaatttc atcgctgctg gttatgacaa
    11821 cgttatgggc attgccaata atggcggcat caatttgcca tgagctatag ggaatgacat
    11881 caataaagga agcggggtcg atcatgacga cataatgtgc cgttcccatg gcgaccatgt
    11941 aacggataat gcctaaatcg ttatgcgatg ttaaccagac acgataacca tctttcgaaa
    12001 ttttacctgg ctcggggaag gtatcgggcg gactttcatg ctccagagac gaacactggg
    12061 gaacgttgtt atcgatataa accacttcct gaatatagcg ataactgtaa gatacccgac
    12121 gcatttccat gagatgggct tcgctacagg cagcgccttg ccatctttcc agctcttgca
    12181 gcgcatcttt cccttgtgtc gccaccttat tggcccgaat agcgacgcgg gaggaatagg
    12241 tatccagctc ttcaataaac gatgtttcta cctgctgatg ggccagccag atgcttaagc
    12301 cgacaggcag caatactgaa agaatcagta ctcccgaaat aaggccgacc agatgtcgtg
    12361 ttctcaccag catatcctta ttaacacagt gtagggaaag tatatctgat tgtggaaaaa
    12421 ggctggcagg agataaggag gtagagctgg tatttcatga agtgatgggt ggaaacgatc
    12481 attgtcattt gaccaggcgc agagctttac gcctggtatg taaccctaac ttaagatgtt
    12541 gattcggcgc gagcgagggt aaaaatatcg taaaaagaca gttcaccttt acggacgatc
    12601 attttttgca gttgttcctt atgaacatcc gcgacacgcg gagagttcaa gatggcttca
    12661 accagcgcgg caggtacaaa gcgcgtgttg taccattcgc cgttatagca aacgcggaaa
    12721 tccagcacgt cgatatcttc atagcggtag cgtgggtaga catcgaaaaa gaagaaacca
    12781 ttaagcagga taaataatac aaccagtagc cagacagagc ctgaaagcgt ttcggactgc
    12841 aacatcaccg ccagggtggc aaaaaaagca acgtacatac cgataaacag ccccggatgt
    12901 ttacggataa aactgatact aaagcggggt ttgttgtcgc gtttttcgcg ggtattcaga
    12961 gactcaatcg tttcggtcag caggcgttgt atttcggtca tcgtttgcct tcgtggtatc
    13021 tgcaactttc cagggaacac cctattttaa cgtgggaata gtagagggga agtaacagat
    13081 ttaccacaat tttgcataac agttgcgaaa aaccgattac aattttttaa ttattctggc
    13141 tggattaccg cccacgacaa cgttgttcgg gacatctttt gtgacaactg cacctgaggc
    13201 taccacgacg ttatcaccaa tggtcacacc agggttaatg accgcgcgtc cgccaatcca
    13261 gacgttatta ccgatggtga cgggtttccc cagttcagca ccgctattac gtgctacagg
    13321 gtcgatggga tgtgttgccg tgtagatatg aacgcctggt gccaacatac agttatcacc
    13381 gatgcgaata gggcagacat caagcatcac gcaatcgaag ttggcgaaaa aattattacc
    13441 gagaaaaatg ttatagccat agtcacagcg aaacgttggc tcaatataag cctctgtcac
    13501 ctgaccgaat agatcagcga gaatttgctg gcgtaatgtg tgctcttccg ccagggaatg
    13561 attgtatcgg tgaataagct gacgagcgcg caggcgatcg cgagataacg tctcatctgc
    13621 cgagcgatac aactcaccag caatcatctt ttctttttct gtgctcatta ctgacctccg
    13681 gttgctgaaa atgcggcaac acagtaagac gaaaagtcag tgaagagaac gttcccatta
    13741 catctgtgtg ataaagatca catagggtat gtcttcatgg cgaaaaagta taaaattctt
    13801 aataaacagc cggttatagc tccgaaagcg aattatttat tagcgaataa atttccaaac
    13861 tgaggaaggg atcttgtcgt acagtttatt catggtcaat tcggcgaggc ggtgatctgc
    13921 ggctgagtaa aataccgcca gttcattatc tgataattcg tatttatttt tctcgataac
    13981 acgctccagc gtgtcaattg tctggcaacg acgtaaacgc attaaataat cggttttcgt
    14041 taaaggtttt tcggacatac ttctacccat ggttgtaata attctaacaa gataaactcg
    14101 caggattctc tttcgtcgca ttgacaaaac aacggaatag tcgattacct gacttccgcc
    14161 atttctgaag atcctgcata ttaataccat aactactgaa caacataaag gtgtcatcca
    14221 gatattcgtc gatctgctca atgagcttat tgtcttcatt atacttaatt ttgtaattaa
    14281 gtgcgaaggt cgcaatatgc tcaatcagtt cattcaactg gaggttgatc gccgaggttg
    14341 ggtcgttaac ccagccatga ttgctttctt caaggtttgc aaggcagtca tgatacaggg
    14401 tttcacagag aaacttaagc tgtgcgatat catgtctttt gggtgagtat tcatccataa
    14461 cgcgtcccct tcttagcggt tgaactaacg gacacctttc gggatggaaa aaacttactg
    14521 acctggactt gccctctcgt ttgttagctt aactataagc cactctttgc aggttttcat
    14581 cgcattatta cgaaaaatta caattaggaa ggaatcggga caggactcac tttttcatac
    14641 tagcactaac ccgcagcagg taaaagcagt ttgctcgaac tgttcataac atttcaggat
    14701 tatcgccaga tgaaattggt gacactaata ccaggattgc tctgaatatg acgtaataac
    14761 cgaggaatga ataaagaatt accgcaaata attaagaata gcctctaaat gattatggat
    14821 tattaaatct attagcgcta cttaatataa attaactaac aatcagtatt ttttatgaat
    14881 tttatccgtg gttaatactg gttttcgtat gagatcctga gttggtggtt caattactcc
    14941 ttaatgttcg taggttatgc ataaaaaagg ccgcttgcgc ggccttagtg attacacgtt
    15001 gtatcaatga tgatcgacag tatggctgtg ctcgatatct tcattcttgc ggctaaagcg
    15061 gcggcgaacc acaacaaaga ataccggaac gaagaagatt gccagtaccg ttgcggtcac
    15121 catcccgccc attacaccgg tacctactgc gttctgcgcg ccggaaccag caccagtact
    15181 gataaccagc ggcataacgc cgaggataaa cgccagcgag gtcatcagga tcggacgtaa
    15241 acgcatccgc actgcatcaa gcgtcgcttc aatcagacct ttaccttctt tatccatcaa
    15301 gtctttggcg aattcgacga taagtatcgc gttcttcgcc gacaacccaa tggttgtgag
    15361 caggcctacc tggaagtaaa cgtcattggt caggccacgg aaggtggcag ccagcaacgc
    15421 accgataacc cccagcggaa cgaccagcat aacggagaac ggaatcgacc agctctcgta
    15481 cagcgccgcc agacacagga atacgacaat caacgaaatc gcgtacagtg aaggtgcctg
    15541 gttgccggag aggcgttcct gataggacat ccccgtccag tcatagccaa caccggtagg
    15601 cagtttgctc gccagttgtt ccattagctc cattgcttca ccggtacttt tacccggtgc
    15661 cgcctggcct aagatttcca tggatggcag gccattgtaa cgttccagac gcggcgaacc
    15721 gtactcccaa cgagaagagg agaatgccga gaacggcacc atctgaccat cagcagcacg
    15781 aacataccag tcgccgatat catccggcag catacggtat ttcgcttctg acatgacgta
    15841 aactttcttc acacgaccgc ggtcgataaa gtcgttcaca tagctaccgc cccatgcagc
    15901 gcccagagtg gtgttaatgt cgttgataga aacacccagc gcctgcgctt tttcctggtc
    15961 gatatcaatc ttaaactgcg gggtatcttc cagaccgttt ggacgtacgc tggtcagcat
    16021 atcagggtgc ttcgctgctt ctgcaagcag ctggttacgc gcctgagtca gtttttcgtg
    16081 accaaggcca gcctggtcaa tcagctcaaa gtcaaagccg gttgcagtac ccagttccac
    16141 gattgcgggc aggttaaagg cgaaaaccat cgcatctttg atttgcgaga aagcgcgtgt
    16201 tgcacgcatg gtaatcgctt caactttgtt ttcttcgccc ggacgatcgg cccagtcctt
    16261 caaggaaacg aacgcaatac cggtattctg accacgtccc gcaaagccga agccgttaac
    16321 ggcgaacacc gactcaacgt tgttcttttc tttggtcaga tagtaatgcg ttacctcatt
    16381 gagcactttc tgcgtacgtt cctgcgttgc acctgctggc agctgaacca tggtcataaa
    16441 tacgccctgg tcctcatctg gcaagaagga gcttggcaga cgcacgaaca gataggccat
    16501 gccgaccacg atgatcagat acagcaccag gtaacgcccc gtactgcgca gaataccgcc
    16561 tacgctgtcg gtgtagtggt gcgtgctctt ctcgaacatg cggttaaacc agccgaagaa
    16621 gcctttttta ccttccccgt gatcgccttt ggcaatcggt ttcagcatgg tggcacaaag
    16681 agccggagtc aggatcaacg ctaccagtac cgacagcgcc attgctgaaa caatggtaat
    16741 agagaactga cgatagatag caccagtaga accgccaaag aaggccatcg gtacgaatac
    16801 cgccgacagt accatcgcga taccgaccag agcgccctga atctgcccca tcgacttacg
    16861 ggtagcttct tttggcggca aaccttcttc cgccataaca cgctccacgt tttctaccac
    16921 aacgatagcg tcatccacca acaagccgat agcgagcacc atcccgaaca ttgttagcgt
    16981 gtttatcgag aagccaaagg cggcaaggac ggcaaaggtc ccgagcaata ccaccggtac
    17041 ggcaatggtc ggaatcaacg tcgcgcggaa gttctgcagg aacagataca taaccaggaa
    17101 cacgaggatg atcgcttcga ccagcgtttt aaccacttcg tgaatagaga ttttcacgaa
    17161 cggcgtggtg tcgtacgggt aaacaatttt cagacccgac gggaagaacg gttccatctt
    17221 cgccagttca gcacggattg ccgcagcggt atccagcgcg tttgcaccgg tcgccagctt
    17281 gatccccaga ccggaagccg gttggccgtt aaactctgcg atgatgtcgt agttctcacc
    17341 acccagctca atcttcgcca catcacgcag cagtacgcgg gaaccatctt gattcacttt
    17401 cagcaggatt ttgccgaact cttcagtaga ggtcagacgc gtctgagcaa taatagaggc
    17461 gttaagctgt tggcctttca ccggcggcgt accaccgagc tgacccgccg caacctgggc
    17521 gttctgcgct ttgatggcgg taatgacatc aaccggcgtt agctggaatt tgttcagctc
    17581 attcgggttc atccagatac gcatcgcgta ctgtgaaccg aacaactgaa catcacccac
    17641 gcccgacgta cggctgatgg catctttcat attcgccgct acgtagtcag agatatcctc
    17701 ctgcgtcatg gtgccatcgg tgttgataac gccgacaacc atcaggaagc tgctggatga
    17761 tttctcaacg ctcacccctt gctgctgaac ttcttgcggc agcaacggca tcgccagctg
    17821 cagtttgttc tgcacctgaa cctgcgcgat gtccgcatca gtaccagact caaaggtcag
    17881 ggtgatctgc acggtacccg tggagtcact gttagaggac atgtacatca ggttatcgat
    17941 accgttcata ttctgttcga taacctgtgt caccgtgtcc tgcactgttt tcgcatcagc
    18001 gccggggtag gaggcggaga tcgttactgc cggcggtgca atcgtaggat attgcgccac
    18061 cggcagtttg aggatcgcca gcccccctgc caacatgatg ataatggcga tcacccacgc
    18121 aaaaatcggg cgatcgataa agaaattagg catgtcttaa cggctcctgt ttaagttaag
    18181 acttggactg ttcaggctga gcaccgcttg cggcttgctg gttattatca gcggtaactt
    18241 cttgtgcttt tacctggaca ccaggacgca ctttctgcag cccacttatt actacgcgat
    18301 cgcctgcttt cagaccttct gtcaccagcc acttatcgcc aatagcctgg cttgcaacga
    18361 tcggacgggt ttccactttg tcatccgcgc caactaccag tacggtggca tcaccacgcg
    18421 gcgtacgggt tacgccctgt tgcgggacta aaatagcgtt tggattaagc ccttcttcca
    18481 gacgtgcgcg cacgaacata cccggcagca gagtgtgatc cgggttcggg aagatagcgc
    18541 gtagggtgat agacccagtg gtctgatcaa cggtaacgtc agagaattcc agcgtaccgt
    18601 cctgcgggaa cttaatgccg tcactggtga tcagcgacac tttggctttg ccgttctctt
    18661 gtttcagcgt gccattcgcc agttcctgtt tcaggcgcag gaagtcgttg ctggactggg
    18721 tcacatcaac gtagatcgga tcaagttgct gcacggttgc cagcgcagtc gcctgaccgt
    18781 tctgtaccaa tgcgccttcc gtcacgttcg acttaccaat gcgaccgcta atcggagagg
    18841 tgactttggt gtaagccaga ttgatccgcg cagtttcaac ggcagctttc gccgcagtta
    18901 ccgcagcatt cgcctgttgc gcatcagcca gagcctgatc gtactcttgc ttactgatgt
    18961 actgagtacc gagcagtttc tgataacgat tcaccgtcaa ttgcgcgata ttggctgcag
    19021 cctgggcttt cgccagatca cctttcgcac tgtcgtatgt cgcctgatag gtcgcaggat
    19081 caatctgata gagagagaca ccagcttcga tgtcgctacc ttctttgaaa ttacgcttca
    19141 ggataatccc gctaacttga ggacgaactt ctgcgatccg gtaggcactg gtgcgacccg
    19201 gaagctcggt tgtgatctgc agaggttcag ttttgactgt tactacgcca acggcgggca
    19261 tctgctggcc accttgttgg gcctgtttgt cgtcacatcc tgttagggct aagctgcctg
    19321 agagcatcag aacgaccgcc agaggcgtaa accctctgtt tttgttcata tgtaaacctc
    19381 gagtgtccga tttcaaattg gtcaatggtc aaaagttaat aaacccattg ctgcgtttat
    19441 attatcgtcg tgctatggta catacattca caaatgtatg taaatctaac gcctgtaaat
    19501 tcacgaacat atggcacgaa aaaccaaaca agaagcgcaa gaaacgcgcc aacacatcct
    19561 cgatgtggct ctacgtcttt tctcacagca gggggtatca tccacctcgc tgggcgagat
    19621 tgcaaaagca gctggcgtta cgcgcggtgc aatctactgg cattttaaag acaagtcgga
    19681 tttgttcagt gagatctggg aactgtcaga atccaatatt ggtgaactag agcttgagta
    19741 tcaggcaaaa ttccctggcg atccactctc agtattaaga gagatattaa ttcatgttct
    19801 tgaatccacg gtgacagaag aacgacgtcg attattgatg gagattatat tccacaaatg
    19861 cgaatttgtc ggagaaatgg ctgttgtgca acaggcacaa cgtaatctct gtctggaaag
    19921 ttatgaccgt atagaacaaa cgttaaaaca ttgtattgaa gcgaaaatgt tgcctgcgga
    19981 tttaatgacg cgtcgggcag caattattat gcgcggctat atttccggcc tgatggaaaa
    20041 ctggctcttt gccccgcaat cttttgatct taaaaaagaa gcccgcgatt acgtcgccat
    20101 cttactggag atgtatctcc tgtgccccac gcttcgtaat cctgccacta acgaataacc
    20161 ctgaatctga ctccaggatt tttcctggac attttcgtcg ttgctattct ggttcactgc
    20221 gtcgtgatat tcttgcggtt tgactttttc aggtcgttct tcaggttcag aaaccttcat
    20281 tcatcatgac tatgttccag tattacaaac gatcacggca ttttgttttt tcagcattta
    20341 ttgcttttgt ttttgtcttg ttatgccaga acacggcgtt tgcgcgggcg tcatcgaatg
    20401 gtgatctgcc gacaaaagcg gacctgcagg cgcaacttga ctcactaaat aaacaaaaag
    20461 atctttctgc tcaggacaaa ctggtgcagc aggatctgac agatacatta gccaccctcg
    20521 ataaaatcga tcgcataaaa gaagagacag ttcagctacg gcaaaaagtc gctgaagcgc
    20581 cggaaaaaat gcgccaggcg accgcggcgt taacagcact tagcgatgtc gataacgacg
    20641 aagaaacgcg caaaattctg agtacgcttt cgttgcgcca gctggaaact cgcgttgccc
    20701 aggcgctgga cgatttgcaa aacgcacaaa acgatctggc gtcttataac agccagctgg
    20761 tttcgttaca gacgcagccc gaacgcgtgc aaaatgcgat gtataacgct tcgcagcagc
    20821 tgcaacaaat tcgcagtcgt ctggatggga ctgatgtcgg cgagactgcc ttacgtccca
    20881 gccagaaagt gttaatgcag gctcagcagg cgttgctgaa tgcggagatt gaccagcagc
    20941 gtaaaagcct ggaagggaac accgtcttgc aggatacctt gcaaaagcaa cgtgattacg
    21001 tgacggcgaa cagcgcccgt ctggagcacc agttgcagct gttgcaagaa gcggtaaaca
    21061 gcaagcgcct gactttaact gaaaaaacgg cgcaggaagc cgtctccccg gatgaagccg
    21121 cgcgtattca ggctaatccg ctggtgaaac aggaactgga aattaaccag cagttaagtc
    21181 agcgtctgat taccgcgact gaaaacggta atcagttgat gcagcaaaac attaaagtca
    21241 aaaactggct ggagcgggcg ctgcaatcgg aacgcaatat taaagagcag attgccgtcc
    21301 tgaagggcag cctgctgttg tctcgtatcc tttaccagca acaacaaacg ctgccctcgg
    21361 cggatgaact ggaaaacatg accaaccgca tcgcggattt gcgtctcgaa cagtttgaag
    21421 ttaaccagca gcgtgatgca ctcttccaga gcgatgcgtt cgtcaacaaa ctggaagaag
    21481 gtcacaccaa cgaagtcaac agcgaagttc acgatgcgtt attgcaagtg gttgatatgc
    21541 gtcgcgaatt gctggatcaa ctcaacaaac agttgggtaa ccagctgatg atggccatta
    21601 acctgcaaat caaccagcag cagttaatga gcgtatcgaa aaacctgaaa tccatcctga
    21661 ctcagcaaat cttttgggtg aacagtaacc gtccaatgga ctgggactgg atcaaagcgt
    21721 tcccgcaaag cctgaaagat gaatttaagt cgatgaaaat cacggtgaac tgggaaaaag
    21781 cctggcctgc cgtttttatc gctttcctcg ccggtttgcc gctgctgttg attgccgggc
    21841 tgatccactg gcgtctgggc tggctgaaag cgtatcaaca gaaactggct tccgctgtgg
    21901 gttccctgcg taacgatagc cagctcaaca caccaaaagc gatccttatc gacctgatcc
    21961 gcgcgctgcc ggtgtgcctg attattctcg cggttggcct gattctgttg accatgcagc
    22021 tcaacatcag cgaactgcta tggtcgttca gtaaaaaact ggcgattttc tggctggtgt
    22081 ttggcctgtg ctggaaggta ctggagaaaa atggcgttgc cgtgcgtcac ttcggcatgc
    22141 cggagcagca gaccagccac tggcgtcggc aaattgtccg catcagtctc gcgttgctgc
    22201 ctatccattt ctggtctgtg gtggcagaac tttccccgct gcatctgatg gatgatgtgc
    22261 tggggcaggc gatgattttc ttcaacctgc tgctgattgc cttcctggta tggccaatgt
    22321 gccgcgaaag ctggcgtgat aaagagtcgc acaccatgcg actggtcacc attaccgtgc
    22381 tgtcgattat cccgattgca ttgatggtgc tgactgccac gggttatttc tacaccacgc
    22441 tgcgcctctc cgggcgttgg attgaaaccg tttatctggt gatcatctgg aacttgctct
    22501 accagacggt attgcgcggc ttaagcgtag cggcgcggcg tatcgcctgg cgtcgtgcgc
    22561 tggcgcgtcg gcagaatctg gtgaaagagg gcgcagaagg tgccgaaccg ccggaagaac
    22621 ccaccattgc actggaacag gttaaccagc agacgctgcg tattaccatg ttgctgatgt
    22681 ttgcgctgtt cggtgtcatg ttctgggcaa tttggtccga tttgatcacc gtgttcagct
    22741 atctcgacag catcacgctc tggcattaca acggcactga agctggcgct gcggtggtga
    22801 aaaacgtcac catgggcagt ctgttgtttg cgattatcgc ctcaatggtg gcctgggcgt
    22861 tgattcgcaa cctgcctggt ttactggaag tgctggtgct ctcgcgactg aatatgcgtc
    22921 agggcgcgtc gtatgccatt actaccatcc ttaactacat cattattgcc gttggtgcga
    22981 tgacggtgtt cggatcgctg ggcgtctctt gggataaact ccagtggctg gccgcagcat
    23041 tatccgtagg tcttggtttt ggtttacaag aaattttcgg taacttcgtc tccggtttga
    23101 tcattctgtt cgaacgtccg gtgcgtattg gcgatacggt aaccattggt agcttctcgg
    23161 ggacggtaag taagatccgt attcgtgcga caacgattac cgatttcgat cgcaaagaag
    23221 tgatcatccc gaacaaagcg tttgttaccg agcgtctgat caactggtcg ttgactgaca
    23281 ctactacgcg tctggtgatc cgtctcggcg tggcctatgg ctccgatctg gaaaaagtgc
    23341 gtaaagtgtt actgaaggcg gcgactgagc acccaagggt gatgcacgaa ccaatgccgg
    23401 aagtcttctt tacggcgttt ggtgccagca cgttggatca tgagctgcgt ctgtatgtgc
    23461 gtgaactgcg tgaccgtagt cgtactgtcg atgagctgaa ccgtactatc gatcagctgt
    23521 gccgtgaaaa cgacatcaac attgccttta accagcttga agtgcatctg cacaacgaga
    23581 agggcgatga ggtgacggaa gtcaaacgcg actacaaagg cgatgacccg acgccagcgg
    23641 tagggtaata accaaagggg gcgacattta gttgccccga gattactgac aaagtgcgcg
    23701 ttgttcatgc cggatgcggc gtgaccgcct tatccggcct acgaaaccgc aagaattcaa
    23761 tgtattgcag gagcggtgta ggcctgataa gcgtagcgca tcaggcagtt ttgcgtttgc
    23821 ccacaatctt aggggacatt tagcggcccc atttatttct cacttttccg cctcatcatc
    23881 gcgcgttaat ttcttttcat aatcgcgttt tacaatatcc agcgcgcgca gcacggtgcg
    23941 ggctgggatc tgattttctt ccagcagcac aatcaaatcg acagccagtt tgacatcgtc
    24001 aggggcattt tccagtgaca tactctctcc attgctagcg ggttaagcgc gctagcctgt
    24061 tttcgatttt ttccagcgca tggcggcagc gtgccaggcg cgcttcatag gcttccactt
    24121 cacgatgcag cgtttgctgt tccacgagat cggtcacccg cgccaggcgg gctctgcgtt
    24181 cggcaaccat ctcacgtagc cgccgctcaa aatcctgatg ctgaatacgt ttacgctgcc
    24241 agcgggcaat tttcggtggc gcactgtccc actcgcgcaa tgaccaggcg gaggcttcac
    24301 gcgcgatggc ttccagttgt gccgccagat gttccgccag ccaggccact tgcggcagct
    24361 gttgctgctc aactgcatga cgaagcgcag ccagattatc gcccgcctca tcgagacaag
    24421 cctgcagtgt tgtcgcacgg gtttgaaaaa ggtgtctgtc aaaacgggca cttagcgtgg
    24481 caaattgcgc taccggggca caacgctgac gcagcgtagc gagctgtcct tccagttttt
    24541 ccagcagcag ggcggttttc acgataagac ctcaatgaaa atgataattg ttatgctaaa
    24601 gtagccactc tgtaagctga caacactcaa tatgcaacga atcattttaa tcatcattgg
    24661 ctggctggcg gtagtgctgg gtacgctggg cgtggtatta ccggtattac cgacgacgcc
    24721 gtttatcctg ctggcggcct ggtgctttgc ccgttcttcc ccgcgctttc acgcctggtt
    24781 gctgtaccgc tcatggtttg gcagctatct acgtttctgg cagaaacatc atgcgatgcc
    24841 gcgcggcgtc aaaccgcggg cgattttgct tattttgctc acgtttgcca tttctctgtg
    24901 gttcgtccag atgccatggg tgcgcatcat gttgctggta attctcgcct gtttgctttt
    24961 ctatatgtgg cgaattccgg tgattgatga aaagcaagaa aagcactgaa gcacaacaat
    25021 cgcagttgca attattgcgt acagccagta cattctggcg ttttcgagca caggcgcagg
    25081 cggtcaaagg ttaaacaact gttacttttg atacgtttaa aacgcgccgt gagtaccacc
    25141 gtaacaagca ggcatacact tatgaccgcg actgcacagc agcttgagta tctcaaaaat
    25201 agcatcaaaa gcattcagga ctacccaaaa cccggcattc ttttccgcga tgtcaccagc
    25261 ttactggaag acccgaaagc ttacgctctc agcatcgact tgctggttga gcgttacaaa
    25321 aatgcgggca ttaccaaagt tgtcggcacc gaagcgcgtg gcttcttgtt tggcgctccg
    25381 gtagctctgg gtctgggcgt tggctttgta ccggtccgta aaccgggcaa actgccgcgt
    25441 gaaaccatca gtgaaactta cgacctggaa tacggcaccg atcagctgga gatccacgtt
    25501 gatgccatca aaccgggcga caaagttctg gtggtggacg atctgctggc aaccggcggc
    25561 actatcgaag cgaccgttaa actgatccgt cgtctgggtg gtgaagtggc tgacgctgcg
    25621 ttcattatca acctgttcga tctcggcggc gaacagcgtc tcgaaaaaca gggcattacc
    25681 agctacagcc ttgtcccgtt cccgggccat taattatcgc cagtcttgtg ctgcccacgc
    25741 tacggacagc acaagatgtg cattcagcct cgccgttctg acggggctgt gttagcatta
    25801 ccccttcgtg aatccacctt ccagcgtttc agagcctgcc aatgagttat caggtcttag
    25861 cccgaaaatg gcgcccacaa acctttgctg acgtcgtcgg ccaggaacat gtgctgaccg
    25921 cactggcgaa cggcttgtcg ttagggcgta ttcatcatgc ttatcttttt tccggtaccc
    25981 gtggcgtcgg aaaaacctct atcgcccgac tgctggcgaa ggggctaaac tgcgaaaccg
    26041 gcattaccgc gacgccgtgc ggcgtgtgcg ataactgtcg tgaaatcgag caggggcgct
    26101 ttgtcgatct gattgaaatc gacgccgcct cgcgcaccaa agttgaagat acccgcgacc
    26161 tgctggataa cgtccagtac gctccggcgc gtggtcgttt caaagtttat ctgatcgacg
    26221 aagtgcatat gctgtcgcgc cacagcttta acgcactgtt aaaaaccctt gaagagccgc
    26281 cggagcacgt taagtttctg ctggcgacga ccgatccaca gaaattgccg gtgacgattt
    26341 tgtcgcgctg tttgcaattt catctcaagg cgctggatgt cgagcaaatt cgccatcaac
    26401 ttgagcacat cctcaacgaa gaacatatcg ctcacgagcc gcgggcgttg caattgctgg
    26461 cgcgcgccgc tgaaggcagc ctgcgagatg ccttaagtct gaccgaccag gcgattgcca
    26521 gcggtgacgg ccaggtttca acccaggcag tcagtgcgat gctgggtacg cttgacgacg
    26581 atcaggcgct gtcgctggtt gaagcgatgg tcgaggccaa cggcgagcgc gtaatggcgc
    26641 tgattaatga agccgctgcc cgtggtatcg agtgggaagc gttgctggtg gaaatgctcg
    26701 gcctgttgca tcgtattgcg atggtacaac tttcgcctgc tgcacttggc aacgacatgg
    26761 ccgccattga gctgcggatg cgtgaactgg cgcgcaccat accgccgacg gatattcagc
    26821 tttactatca gacgctgttg attggtcgca aagaattacc gtatgcgccg gaccgccgca
    26881 tgggcgttga gatgacgctg ctgcgcgcgc tggcgttcca tccacgtatg cctctgcctg
    26941 agccagaagt gccacgccag tcctttgcgc ccgtcgcgcc aacggcagta atgacgccaa
    27001 cccaggtgcc gccgcaaccg caatcagcgc cgcagcaggc accgactgta ccgctcccgg
    27061 aaaccaccag ccaggtgctg gcggcgcgcc agcagttgca gcgcgtgcag ggagcaacca
    27121 aagcaaaaaa gagtgaaccg gcagccgcta cccgcgcgcg gccggtgaat aacgctgcgc
    27181 tggaaagact ggcttcggtc accgatcgcg ttcaggcgcg tccggtgcca tcggcgctgg
    27241 aaaaagcgcc agccaaaaaa gaagcgtatc gctggaaggc taccactcca gtgatgcagc
    27301 aaaaagaagt ggtcgccacg ccgaaggcgc tgaaaaaagc gctggaacat gaaaaaacgc
    27361 cggaactggc ggcgaagctg gcggcagaag ccattgagcg cgacgcgtgg gcggcacagg
    27421 tgagtcaact ttcgctacca aaactggtcg aacaggtggc cttaaatgcc tggaaagagg
    27481 agagcgacaa cgcagtatgt ctgcatttgc gctcctctca gcggcatttg aacaaccgcg
    27541 gtgcacagca aaaactggct gaagcgttga gcacgttaaa aggttcaacg gttgaactga
    27601 ctatcgttga agatgataat cccgcggtgc gtacgccgct ggagtggcgt caggcgatat
    27661 acgaagaaaa acttgcgcag gcgcgcgagt ccattattgc ggataataat attcagaccc
    27721 tgcgtcggtt cttcgatgcg gagctggatg aagaaagtat ccgccccatt tgatcgtaag
    27781 cacagcttac gttcgtcatc cttaacgtga ttgagagaga aacctatgtt tggtaaaggc
    27841 ggtctgggta acctgatgaa gcaagcccag cagatgcaag aaaaaatgca gaaaatgcag
    27901 gaagagatcg cgcagctgga agtcaccggc gaatctggcg caggtctggt aaaagtgacc
    27961 atcaacggtg cacacaactg ccgtcgcgta gagatcgacc cgagcctgct ggaagacgac
    28021 aaagagatgc tggaagacct ggtggctgca gcattcaacg acgcagcacg tcgtattgaa
    28081 gaaacgcaga aagaaaaaat ggcctctgta tcctccggaa tgcagctgcc gcctggcttt
    28141 aagatgccgt tctgatgcaa accagcccgc tgttaacaca gcttatggaa gcactgcgct
    28201 gtctgccggg cgttggcccg aagtcggcgc agcgtatggc gttcacgctg cttcagcgcg
    28261 atcgtagcgg cgggatgcgt ctggcgcagg cgctcacccg ggcgatgtcg gaaatcggcc
    28321 actgcgccga ttgccgcact ttcaccgaac aggaagtctg taacatctgt tcgaatccgc
    28381 gtcgtcagga aaacggtcaa atctgtgtgg tggagagtcc ggcggacatc tacgccattg
    28441 agcagacggg gcagttttca ggccgttatt ttgtgttgat ggggcacctg tcaccgctgg
    28501 acggcatcgg tccggatgat atcggccttg atcgtctgga acagcgtctg gcagaggaaa
    28561 aaatcactga agtgatcctc gccaccaatc ccacggttga aggtgaagct accgctaact
    28621 acattgccga gctttgcgcg caatatgacg tggaagccag ccgaatcgct catggcgtac
    28681 cggttggcgg cgaactggaa atggtcgatg gcaccacgct gtcacactcc cttgccgggc
    28741 gtcataagat tcgtttttaa gcaaacga
//
LOCUS       DAMZET010000042        28037 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.042, whole genome shotgun sequence.
ACCESSION   DAMZET010000042 DAMZET010000000
VERSION     DAMZET010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.042"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            109..570
                     /locus_tag="POB34_001924"
     CDS             109..570
                     /locus_tag="POB34_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918883.1"
                     /translation="MSLALHGKSELELAAQKALELLDYVSDITTVDGKILAIMGLITG
                     LSGQAKVSHILFEQAKIHSTDIASLYYYRALVHFHNEKIEEARICIDKSLQLEPRRRK
                     AVVIKECVDMYVPNPLKNNIKLYYKETESESHRVIIDNILKLKQLTRICMR"
     gene            738..956
                     /locus_tag="POB34_001925"
     CDS             738..956
                     /locus_tag="POB34_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918884.1"
                     /translation="MTNPIGINNLSQSSNIANATGDEVVSLDKHINTSATDTDQIQAF
                     IVSTWMAPFQNDMYSEDNPISPYYKIEW"
     gene            1039..1515
                     /locus_tag="POB34_001926"
     CDS             1039..1515
                     /locus_tag="POB34_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDI5918885.1"
                     /translation="MRSPKVKFLTIFTFCIFITKMSFASNSCSNEAGTMFRIEPNLIK
                     AIALVESNLKKDSIGKNRDKNNNIKSLDYWLMQINQMHIPLLKKRGIIKDERDLLDNP
                     CLNIKIGTEILYNHFSRCGVTWQCLGTYNAGFAMDNQKKRQQYAPKYILYIPGLMN"
     gene            complement(1560..2192)
                     /locus_tag="POB34_001927"
                     /pseudo
     CDS             complement(1560..2192)
                     /locus_tag="POB34_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(2413..2844)
                     /locus_tag="POB34_001928"
     CDS             complement(2413..2844)
                     /locus_tag="POB34_001928"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918886.1"
                     /translation="MINDLKSILLKSSEEVDVFIKIFESWRKKLPAISGPVNLYIPTR
                     FKDKYLEVESYFVDKSIWNVHITFHDDKRFVFFTDQFIAEFSPQEFVDNCEQYLINNH
                     CFSPDKVNEICEQARHYLVEKMFETHSLDMNNSVLASPEDL"
     gene            complement(2847..3068)
                     /locus_tag="POB34_001929"
                     /pseudo
     CDS             complement(2847..3068)
                     /locus_tag="POB34_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304051.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3061..3642)
                     /locus_tag="POB34_001930"
     CDS             complement(3061..3642)
                     /locus_tag="POB34_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HDI5918887.1"
                     /translation="MNLALRKIIYDPISYIHPQRVSLNNTPINNPVLRSITNEMIVLQ
                     YNLSVEHFNLNSSLIYYINNWNLFPLFCLFSGYHFYRERFAERGFFYKVPAVLRDYLS
                     AIPVKINEKARYKPGIASYHNIITCGFSTLSPYIRQQPLAMQQRFNLLFPDFVDHIQL
                     PLPLASTLLERITFYAKKNRDELDKISCKWCCD"
     gene            complement(3658..4392)
                     /locus_tag="POB34_001931"
     CDS             complement(3658..4392)
                     /locus_tag="POB34_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HDI5918888.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIISSRVHVSYDVDNGDSGKTALPIHISVLAVYEKDINPEI
                     KINDIKRFIVNSSASVQYENISVVLSKRRDIIEQAPTYEISEPVFAYDKAMPVSILLA
                     LISVATCWLLWKYRAILTNLVRLKIK"
     gene            complement(4433..4648)
                     /gene="sctI"
                     /locus_tag="POB34_001932"
     CDS             complement(4433..4648)
                     /gene="sctI"
                     /locus_tag="POB34_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HDI5918889.1"
                     /translation="MIDLNDRVLNLDNPDDKMISAFANYAVQTENWQQNALQALRSDK
                     EGLTPEKLLVLQDHVLNYNVEVSLVEH"
     gene            complement(4740..4980)
                     /locus_tag="POB34_001933"
                     /pseudo
     CDS             complement(4740..4980)
                     /locus_tag="POB34_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            complement(4994..6175)
                     /locus_tag="POB34_001934"
     CDS             complement(4994..6175)
                     /locus_tag="POB34_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HDI5918890.1"
                     /translation="MENNDKFLSQELLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGRSDAFSELPENTIVVPYGELTGSFEIIITTDPDLVVTFRELTAQEPEDRTLTLN
                     QQIEVLGLKFAVKEKNEVWQYSLPGIIENNIISTKQHFFSSKLFKYVMLFFLFAIIFI
                     AFYIVNASNDPQLRHIDKILVNKNRNYEILYGRDHVIYINTNILDEAVWVKQALEKNQ
                     PGKPVRVINPDDESIRIFSWLADNFPDLQYFKLQLLDASNLRLTVSKQRNAITQQLID
                     NLIKGLLQTMPYASNISIAVLDDNVLESQAIETLSAIGLSYEKYKTANNVYFNIIGTL
                     SDSELNKINNYVDEYYKQWGKQYVRFNVNLKNQDTNNSSFSYGDNRFEKSQGSNWTFQ
                     E"
     gene            6433..6933
                     /locus_tag="POB34_001935"
     CDS             6433..6933
                     /locus_tag="POB34_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HDI5918891.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLNPNTEPVNILLSNNFIRINKNITLSDLTHFLISNLHTQNVYSTQEPLTHDEID
                     ILRLCVSYSLKQIAIIKGIDYKTISYHKIRALNKLNIKGTVELFIALCEWDKHYFKLQ
                     SCVRES"
     gene            6935..7228
                     /locus_tag="POB34_001936"
     CDS             6935..7228
                     /locus_tag="POB34_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HDI5918892.1"
                     /translation="MSLVGYNSNIIITIALISLLSACTIENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLVISQDRLTH"
     gene            complement(7290..7652)
                     /locus_tag="POB34_001937"
     CDS             complement(7290..7652)
                     /locus_tag="POB34_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HDI5918893.1"
                     /translation="MIANPTHIAIGIYFKPHLSPIPLISVRETNEVALAVRKYAKEIG
                     IPIITDKKLARKIYATHRRYDYVSFENIDEILRLLLWLEDVENAGQPVPDEQLSSEDK
                     FIEGEEKEIENKDDNLKN"
     gene            complement(7679..8419)
                     /locus_tag="POB34_001938"
                     /pseudo
     CDS             complement(7679..8419)
                     /locus_tag="POB34_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284706.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(8428..9195)
                     /gene="sctT"
                     /locus_tag="POB34_001939"
     CDS             complement(8428..9195)
                     /gene="sctT"
                     /locus_tag="POB34_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HDI5918894.1"
                     /translation="MEEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALFQHYHYELSTLNEIDIALFAAREIIIGLFIACLLSSPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTNGGLNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLARYASQLNAFAISLTV
                     KSALAFLILIIYFGPILAERVMPLSFFPEQLQLYIEK"
     gene            complement(9197..9457)
                     /locus_tag="POB34_001940"
     CDS             complement(9197..9457)
                     /locus_tag="POB34_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HDI5918895.1"
                     /translation="MNDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(9467..10132)
                     /locus_tag="POB34_001941"
     CDS             complement(9467..10132)
                     /locus_tag="POB34_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HDI5918896.1"
                     /translation="MSNSISLIAILTLFTLLPFIIASGTYFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGTEIYYNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFFEKIQKVNSSEDNEEIIDDDNISIFSLLPAYALSEIKSAFIIGFYI
                     YLPFVVVDLVISSVLLTLGMMMMSPVIISTPIKLILFVAMDGWTMLSKGLILQYFDLS
                     INP"
     gene            complement(10122..11069)
                     /locus_tag="POB34_001942"
     CDS             complement(10122..11069)
                     /locus_tag="POB34_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
                     /protein_id="HDI5918897.1"
                     /translation="MFGLRKVNRNTHMFETTFQNWKENGEDVALLMPEFSAKWLPIAE
                     ESGSWSGWVLLGEIFPLISSELAGMALMPETERLIGEWLSLSSSPLNLKHPELKYNRL
                     RVGKVFDGVLSPAQPLIRIWTGELNLWLDKVTVCQYGNAPALDKKSLYWSIHFVIGFS
                     KTCYRSLIDIEVGDVLLISNNLAYAVIYNTKICDLIYPEELKMADHFEYEEDFETDDF
                     DIKKNESEIYDENDDQMINSFEDLPVKIEFVLGKKIMNLYEIDELCAKRIISLLPESE
                     KNIEIRVNGALTGYGELVEVDDKLGVEIHSWLSGHNNVK"
     gene            complement(11069..11832)
                     /locus_tag="POB34_001943"
                     /pseudo
     CDS             complement(11069..11832)
                     /locus_tag="POB34_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314987.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            complement(11960..12376)
                     /locus_tag="POB34_001944"
     CDS             complement(11960..12376)
                     /locus_tag="POB34_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HDI5918898.1"
                     /translation="MLSKVNRLIRRTAQSLAACEASLQKLNAEKEKLAEKERLYDMQL
                     KNLKSLLDKKELLGEVVFRQDIFYSLRKVAVIQQQIAEINLEKQKIAERRKILNKEIV
                     QQQAQRKHWWLKGEKYVRLKTRIKKTFKSDASSRRA"
     gene            complement(12366..13658)
                     /gene="sctN"
                     /locus_tag="POB34_001945"
                     /pseudo
     CDS             complement(12366..13658)
                     /gene="sctN"
                     /locus_tag="POB34_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
     gene            complement(13690..14238)
                     /locus_tag="POB34_001946"
                     /pseudo
     CDS             complement(13690..14238)
                     /locus_tag="POB34_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
     gene            complement(14220..14351)
                     /locus_tag="POB34_001947"
     CDS             complement(14220..14351)
                     /locus_tag="POB34_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HDI5918899.1"
                     /translation="MIEEGLLLPMNVLLQGQKLTLLDPEMWFLRGNENKDVTIFLIN"
     gene            complement(14697..14876)
                     /locus_tag="POB34_001948"
     CDS             complement(14697..14876)
                     /locus_tag="POB34_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HDI5918900.1"
                     /translation="MINKLLLAYLIGLVVTSTFIFIFSEEKVTYRLFATIITGLTWPL
                     SLIPSIISLMIRKSD"
     gene            complement(14943..16553)
                     /locus_tag="POB34_001949"
     CDS             complement(14943..16553)
                     /locus_tag="POB34_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918901.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHVKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPNP
                     IIPLNWSKASMTLMLKEMSSLINDEGNK"
     gene            complement(16787..16860)
                     /locus_tag="POB34_001950"
     tRNA            complement(16787..16860)
                     /locus_tag="POB34_001950"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16826..16828),aa:Gly,seq:ccc)
     gene            complement(16939..17694)
                     /locus_tag="POB34_001951"
     CDS             complement(16939..17694)
                     /locus_tag="POB34_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDI5918902.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSITRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            18109..20406
                     /gene="xdhA"
                     /locus_tag="POB34_001952"
     CDS             18109..20406
                     /gene="xdhA"
                     /locus_tag="POB34_001952"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HDI5918903.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQIQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            20417..21295
                     /gene="xdhB"
                     /locus_tag="POB34_001953"
     CDS             20417..21295
                     /gene="xdhB"
                     /locus_tag="POB34_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HDI5918904.1"
                     /translation="MFDFASYHRAATLADAINLLVDNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPVTQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHVGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            21292..21771
                     /gene="xdhC"
                     /locus_tag="POB34_001954"
     CDS             21292..21771
                     /gene="xdhC"
                     /locus_tag="POB34_001954"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HDI5918905.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(21811..23589)
                     /locus_tag="POB34_001955"
     CDS             complement(21811..23589)
                     /locus_tag="POB34_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HDI5918906.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVTEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            24068..25255
                     /gene="ygeW"
                     /locus_tag="POB34_001956"
     CDS             24068..25255
                     /gene="ygeW"
                     /locus_tag="POB34_001956"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HDI5918907.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            25313..26509
                     /gene="dpaL"
                     /locus_tag="POB34_001957"
     CDS             25313..26509
                     /gene="dpaL"
                     /locus_tag="POB34_001957"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDI5918908.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            26567..27778
                     /locus_tag="POB34_001958"
     CDS             26567..27778
                     /locus_tag="POB34_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HDI5918909.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            27831..>28037
                     /locus_tag="POB34_001959"
     CDS             27831..>28037
                     /locus_tag="POB34_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918910.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIA"
BASE COUNT         8140 a         6193 c         5468 g         8236 t
ORIGIN      
        1 acccctgaaa gcatttacag ggccatgact atatttgata gattacaaaa taaaagtgat
       61 atacagacgc taaaaacaga atgttattgc cttctagcgg aatgccatat gtctttggca
      121 cttcatggaa aaagtgaact tgaacttgct gctcaaaaag cattagagct tttagattat
      181 gtatcagaca taaccactgt cgatggaaaa attttagcta ttatgggact gataactggt
      241 ctgtctggac aagcaaaagt atctcatatc ttatttgaac aggctaagat acactcaact
      301 gatatagcct ctctctacta ctatagggca ctagtccact ttcataatga aaaaattgaa
      361 gaggcaagga tttgtataga caaatcacta caactcgaac ccagaagacg aaaagcagtt
      421 gtgataaaag aatgtgtaga tatgtatgtg cctaacccgc tcaaaaacaa cattaaactc
      481 tactataaag aaactgagag tgaaagccat cgagttataa ttgacaacat tttgaaatta
      541 aagcagctga cgagaatttg tatgcgataa atttttattg acgcaatttt attatttaga
      601 tgtatgcaac ttgttattta aacaacagag ataaaaatat ctaagatatt caccttattg
      661 caagatattt aaaatgctct agagttaaaa gtatgatgta aagaccattg gcctggacaa
      721 ttaaaaaagg gagctttatg actaatccaa tcggtatcaa taatttatct caaagttcaa
      781 atatagcgaa tgcaacgggt gatgaagtcg tatcattaga taaacacatc aacaccagtg
      841 ctactgatac agatcaaata caagcattta tagtttcaac atggatggcg ccttttcaaa
      901 atgatatgta ttcagaagat aatcctatct caccttacta taaaattgaa tggtagacga
      961 ttgaccaaat aaatcataac gagatacttt tataatagta tctcgttcct ttcattcaat
     1021 cctcacacat gaaaaaatat gcgctcacca aaagttaaat tcttaaccat cttcacgttt
     1081 tgtatattta ttacaaaaat gtctttcgcc agcaactctt gctctaatga agcaggaaca
     1141 atgttcagaa tcgaaccaaa tttaattaaa gctatcgctc ttgttgaaag caatctaaaa
     1201 aaggatagca taggcaagaa cagagataag aataacaaca taaagagttt agattattgg
     1261 ttaatgcaga taaaccagat gcatattccc ctgttaaaaa aacgtggaat aataaaagat
     1321 gaacgagatt tgttagataa cccttgtctg aacattaaaa taggaaccga gattttatat
     1381 aaccattttt cacgttgtgg agtaacgtgg caatgcctgg ggacttataa tgcgggtttt
     1441 gcaatggata accaaaaaaa gagacagcaa tatgccccaa aatatatatt gtatatacca
     1501 ggcttaatga actagacaat cgtaaggctt cagcaaaatg agcctaaagc ctcttttttt
     1561 tatatagtgc aaacacccat acgtaaagct gttttgatca actcgatacc actgtgaaca
     1621 tctaatttct tcatgatatt gagtctatga gtttcgactg tttttctgct taactgcaag
     1681 aaattagcga tttctttgtt tgttttacca tcagctatga gttgtaatat ttctgactca
     1741 cgatttgtaa gttgattatc agaggaatac ctggaagata ttttttcaca atccatatgt
     1801 acactatcaa aataagtgta accattacta atcgaatcaa tagcacgatt tagtttcgcc
     1861 gggtgactgg ttttccatac gcatcctttc gcacctgctt caagcaattt tattgaatca
     1921 atatacggca tctttgctac aaatgtaata atatgagcat ctatcttatg cgatttaacc
     1981 cattttacga gttcggaccc catcattcca cctgcaaatt ctcctaaaat aaatatttag
     2041 gctgtagatt tgttacactc cgctatagat ttcttcagat ctttatagcc cccaacaacc
     2101 tcatataaat caggataatg tccgagaaat ccaattatcc catcaatcat aaacggctgc
     2161 tgatctgaaa ctacaatttt aatttttccc atatattcat cctgagtgta aatgctttaa
     2221 cgcattcatt tataatcatc ccttccatta ttattataag caaaatccaa agaatacatt
     2281 gatgaaataa taatgaaata taattaaaaa taaaattttt gcgtaaaaaa taccacaggc
     2341 attaaaaaat catgagatga ttaaaatatt acaattagat tatattcaaa tcattaaact
     2401 tgagccaggg agctataagt cctcagggct tgccaaaaca gaattattca tatccaaaga
     2461 atgtgtttca aacatttttt caactaaata atgtctggct tgttcgcaga tttcgttaac
     2521 cttgtcaggg gagaaacaat gattattaat taaatattgc tcacaattat ccacaaattc
     2581 ctgcggacta aattcagcaa taaattgatc ggtaaaaaac acaaaacgct tatcatcatg
     2641 aaatgtaata tggacattcc atattgattt gtcgacaaaa tatgactcta cttcaagata
     2701 cttatctttg aatctggtag gtatatagag atttacagga cctgatattg ccggtaattt
     2761 ttttctccaa ctttcaaaaa ttttgataaa aacatcaact tcctctgagg attttaacaa
     2821 aatactctta agatcgttta tcatgtttat tctttttttt ctagttctga gctcaacaag
     2881 cttatcaagg gtataaaatt atccatcaca tcaataatgc cttttgtata gccatcataa
     2941 taaccttgaa taaatttttc ttctatcttt acctcaatgc tctgaatttt agcgctggtt
     3001 tcaagaagga tactttcttt aagagaaact atttatttca atccatcaga aatttttctt
     3061 ttaatcacaa caccatttgc aggagatttt atcaagctca tctctatttt ttttcgcata
     3121 aaatgtaatt ctctctaaaa gagttgatgc aagtggtaac ggcaattgta tgtgatcaac
     3181 aaaatcagga aataataaat taaatcgttg ctgcattgcc aatggttgtt gccttatata
     3241 gggtgacaac gttgaaaaac cacatgtgat gatattgtga tagctagcga tgccgggttt
     3301 gtatcgtgcc ttttcattta ttttcaccgg tatagctgat aaataatccc ttagcacagc
     3361 aggaacctta taaaaaaatc cgcgttctgc aaacctttct cgataaaagt gataaccact
     3421 gaataaacag aacaatggaa ataaattcca attgtttatg tagtaaataa gtgaactatt
     3481 cagattaaaa tgttcaactg aaagattgta ctgcagaaca atcatttcat tggtaataga
     3541 tctaagcacc ggattgttaa taggtgtatt attcaagctt actcgttgag gatgaatata
     3601 actaattgga tcatatataa tctttcttag ggcaagattc ataacgctct catatgtcta
     3661 cttaattttc aacctgacta aattagttag aatggcccta tacttccata acagccagca
     3721 agtcgctacg gatattaatg caagtaagat agaaaccggc atagccttat cataagcaaa
     3781 aacaggttcg ctaatttcat atgttggtgc ttgctcaata atgtctcttc gttttgacaa
     3841 tacaacagaa atattttcat attgtacgct tgcagagcta ttaacaataa atctcttgat
     3901 atcatttatt tttatttctg ggttgatatc tttttcatat actgcaagta cagaaatatg
     3961 aattggtaaa gcagttttac cactatcgcc attatcaaca tcgtaactaa catgtactct
     4021 cgaagaaata atgccatcca taattttgag agattgctct aaccgctgct caatagcaga
     4081 atatagcctt gctttttccg ctcgtggaga tgaaacgagt gcatctgcag ggaacatctg
     4141 cgatatttga atatcaggct tacccggtag attgtagatt tttagccaat ccaccgcaga
     4201 agcaaaatcc gttggttcga caaatattga aaatcctgtt ttgccttgat ccttcttttc
     4261 agcattaata ttatgtcttt gtaaaacagc aaggacatca ttagcttgct gttgatcaag
     4321 atggttcaat aattcctgct gcttgcagcc gcacaacagc aggataaaca ataataatga
     4381 tatatatttc atgagcgggt caacgtctca actgcagcga caatctttct tgctaatgtt
     4441 ccaccaatga aacctcgaca ttataattaa gcacatgatc ctgcaagact aataattttt
     4501 caggagttaa cccctcttta tcagacctta atgcttgtaa cgcattttgc tgccaatttt
     4561 cagtttgtac agcatagttg gcaaaagcgg agatcatttt atcatccggg ttgtcaagat
     4621 tgagaacccg gtcatttaaa tcaatcactt tcgcttgctg tatttcatga gcagttgatt
     4681 actcagctct atcaataggt ggcatattgc ttactgacat aataaaatcc ttatccagtt
     4741 tagcggaagt tttgtatgat tgctgaatca agatctttat acgcttttac aacgttagat
     4801 tgcgcatttc gatataatgt atactcagct aatgcacttt gatattcggc aagaaatttc
     4861 gtgtcggagg gattggttgg ctaaatcctc caacgctttt tcaacttgct ggttcagatc
     4921 atcaacgccc tgatcaaatt gattgctgat atccataata taaccattcc aatctgccat
     4981 aagtcctcct caattattcc tgaaatgtcc agttgctacc ttgagacttc tcgaatcggt
     5041 tatctccgta gctaaatgaa ctattatttg tgtcctgatt tttcaaattc acattaaatc
     5101 ttacatattg tttaccccat tgtttataat attcgtcaac atagttatta attttattta
     5161 attcactgtc acttaacgta ccaatgatat tgaaatacac attgttagct gttttatatt
     5221 tttcataaga aagacctatc gctgacaacg tttcaatagc ctgactttcc aatacattat
     5281 catctaatac cgcaatactt atattgctgg catatggcat agtttgcagt aatcctttaa
     5341 taagattatc gattagttgc tgtgtgatag cgttccgttg cttactcact gtcaatctta
     5401 gattactggc atctaatagt tgaagtttaa aatattgtaa atcagggaaa ttatcagcaa
     5461 gccatgaaaa aattcgtatc gattcatcat caggatttat cacccgtact ggctttcctg
     5521 gttgattttt ttccagtgct tgtttgaccc agactgcttc atccaaaata ttggtattaa
     5581 tatagataac atgatctcta ccatataaaa tttcataatt cctgtttttg tttacaagaa
     5641 ttttatcgat atgtctcagc tgcggatcat tactggcatt aacaatataa aaagcaatga
     5701 aaatgatagc aaaaagaaaa aaaagcatta catacttaaa cagcttactg ctaaagaaat
     5761 gttgttttgt agaaataatg ttattttcaa taatgcctgg caatgaatac tgccaaactt
     5821 catttttttc ttttacagca aatttgagac ctaaaacttc tatttgctga ttcaatgtta
     5881 acgttctatc ttcaggttct tgtgctgtta attctctgaa tgttactacc agatcagggt
     5941 cggtagtaat tattatctca aaactacctg taagctcgcc ataaggaacg actattgtgt
     6001 tttctggtaa ctcagaaaaa gcatcacttc ttcctaacga aacatcatta ccaatgataa
     6061 caagtagacg cccattaagt atttcatact cgcatccatt caatggtccg ctcaacaaac
     6121 gaatggcata agattccaat aattcttgtg ataagaattt atcattattt tccatgaata
     6181 ccacccgcta attccataag ctctagttta gagtagtgta acaagacgga aatatcatga
     6241 ccaaattatg atgacacaga ataaaaaaat tataatttcg agaactattt taaatgcctc
     6301 tatgcataac tttatttaca atttaaggta taaaccctat tccctcaatg ttaagcaaat
     6361 ataacaatta cttttatttt aacttttaca cgtaatattt accctaaatg attttagagt
     6421 acaggttggc atatgcaagt cttttccagt gatgtctact tcactgtagg cactaatgca
     6481 ttattagcat cccaaaaaga atattacagc gatttagttg cgttggttga tcttgggcat
     6541 tcgttcgttg ttattgatga gcatcaacat cgaaatctca atccaaatac agaacctgta
     6601 aacatccttt taagcaacaa tttcatcagg atcaataaga atattacatt atctgattta
     6661 acgcatttcc tgatttcgaa tcttcataca caaaatgtgt attccacaca agaaccactt
     6721 acccatgatg agatagatat cctgcggtta tgtgtttctt actcgttaaa acaaatagcc
     6781 attattaaag gaattgatta taaaacaata tcatatcaca aaataagagc cctaaataaa
     6841 cttaacataa aaggtactgt tgagttgttt atagcactct gtgaatggga taaacattat
     6901 tttaaactcc aatcttgcgt aagagaaagt tgatatgtca ttagtaggat ataatagtaa
     6961 catcataata accatcgcgc tgatatcgtt gctctctgca tgcaccatag aaaattcatt
     7021 aaaggagaat caaaataaaa gaagtggcga atcccaagcg atagtagatg caagacagtt
     7081 ctttagcgaa cacccggaat atatatcttc agcagaactg gagcaaaagc tatatcgcga
     7141 atttgcacag gttactacag ttccagctta taaagagatg agtatgtatc aattgcttgt
     7201 catttcacaa gatcgtttaa ctcattaaat aatgaatcta cacggttttt gcgtgtagat
     7261 tcttataaat ttctgactac gacagatgtt taatttttta aattatcgtc tttattctcg
     7321 atttctttct cttcaccttc aatgaattta tcttctgaag agagctgttc atctggaaca
     7381 ggttgtccag cattctccac atcttcaagc cacagcagaa gacgtaatat ttcatctata
     7441 ttttcgaagc taacataatc gtagcgacga tgggtagcat aaatttttcg tgctaatttt
     7501 ttatctgtga taattggtat tccgatttcc tttgcatatt tccttacggc taaggctacc
     7561 tcattagttt ctcttacaga aatcaatgga ataggtgaaa gatgtggctt aaagtatatt
     7621 cctattgcaa tgtgagtagg gttggcaatc atcaaacgac tattactgac atcagacttc
     7681 aattgctcag aaagaatttc ctgatgcgct ctctgcgttt agacttaatt tctggatttc
     7741 cttcttgctt cttgtattct cttttaactt cttgtttatc cattttcata tctttcataa
     7801 ataaaaaata ttcagcgata aaatcaaaaa ttaagacaat tatcatactg ccgagacaat
     7861 acagtatgag aaggaatagc atctctcccc aatctgataa taaagatatg atatctccat
     7921 caagagtttt aaaaagcagt gatttatgat tacttcaaaa tactttgatc gccaatgcaa
     7981 aaaaaaaaaa taaaataata taaagaattg ctttaacaaa ttcttttacg gtttttaagc
     8041 caaatatacg ctttaagcca tttactggat tcaatgaatc aaacttaaat ttaacagctt
     8101 cagttgctaa ttgcatttta ctttgaaacc aggagacaag tattgttgtg aacatgcaaa
     8161 ccagcaaaaa aggaatgatc gttttcaacc aaccaacccc catagcttta aaatagtccc
     8221 aaatatctgg tttagcgtta tgatcaaaga tgtattcaag aatcgacata atgtgatgca
     8281 catcaaaaac atatccgaga tataaagtac ccacaagcat tattacagag atggttaagt
     8341 ctctactttt tagaatttgc ccttttttag aagcatcctg gagtttcttt tgtgttggct
     8401 tttcagtttt attggccatg aaacactcta tttctcaatg tatagttgca attgttctgg
     8461 gaaaaaggat aacggcatta ctcgttcagc cagtattggg ccaaaataaa taattaatat
     8521 taaaaatgcc agcgcacttt tgacagtcag tgaaatggca aaagcattta gttgtgaagc
     8581 ataacgtgcc agcaatccca ggacagcctc accgcccagc ataacggcaa tcacggggct
     8641 tgcgtaaacg atagtatgag tcattatgtt attaatatat gagaataaag gctctaattt
     8701 aggaaaatta aaattgcctg aaggccataa attataactt tgccaaagag tttccagaat
     8761 aaagttcaat ccaccgttag taagataaac cgctgcagag aataggttaa ataatcttgc
     8821 taattccgaa gtatcgacac cagtagcagg atccaaagta ctacttaacg ttgcaccacg
     8881 ttggttatcg ataaaactac ctatagcaag gaaaatccaa aacggggatg ataataagca
     8941 agcaatgaac agccctatga taatttctct ggcagcaaac aaagcgatat cgatttcatt
     9001 taaggtcgat aattcatagt ggtaatgctg aaataatgca catgatacca ctattattat
     9061 tggatgacgg actactgttg gtatattccc actggcgaaa aatggtaaca aatagaaagt
     9121 tggtgccaat cggcaaaaac cgagcgctgt agcagccagc agactatgca actgatagag
     9181 tatcgcctcc tccatcctat cctctcgcga atgctaaatt cagcatttcg ataccaaacg
     9241 aatataattt ttcaccatac catccgctca ttaaaaaaaa gcaaatactt acgcataata
     9301 atttgacgcc aaatggcaac gtctgttctt gtaactgtgt taccgtttga aaaagcccta
     9361 ccaataaacc aacaaatgtc gcaactgcga taggtcctgc agacataact aaaatcagat
     9421 acagcgccct attccctgca aaaacaatat cgttcatatt ttttcctcag ggattaatgg
     9481 ataggtcaaa atattgtaaa atgagtcctt ttgataacat tgtccaaccg tccatagcga
     9541 caaatagaat taattttatc ggtgtagata tgattaccgg gctcatcatc atcataccta
     9601 acgttaacag aacgctggat ataaccagat ccactacgac aaagggtaaa tagatataaa
     9661 aacctataat aaatgcactt tttatctcac tcaatgcata tgcgggcagt aacgagaaaa
     9721 ttgaaatatt atcatcatcg attatctctt cattgtcttc agaactattc accttctgaa
     9781 tcttttcaaa aaagctaacc aactcaggtt cagaatattt tatcaaatat gatttataac
     9841 cactcatgcc tgtctcaaca aaattaacaa ccgacgctac attattgaat gaaagattct
     9901 cattctgact gttataatat atttccgtcc caaccggcat cattacaaac attgacaata
     9961 gcaaagcaac cccattaagt gtcatatttg atggcacttg ctgaagcccc agcgcgtttc
    10021 ggacaataac aaaaacaatc gagaatttaa taaaatatgt cccggatgct attataaacg
    10081 gcaatagtgt aaataatgtt agtatagcta tcagcgaaat gctatttgac attattatgc
    10141 cctgataacc aagaatgaat ctctacgccc aatttatcat ccacttcaac aagttcgcca
    10201 tagccagtta gcgcgccatt tacacgtatt tctatatttt tctcagattc aggaagcaga
    10261 gatattattc tttttgcaca gagttcgtct atttcatata gattcattat tttcttacca
    10321 agaacaaact caatttttac aggcaagtct tcaaaactat taatcatctg atcgtcattt
    10381 tcatcataaa tctcactctc gttttttttg atatcaaaat catctgtttc aaaatcttcc
    10441 tcgtactcaa aatgatcagc catttttaac tcctctggat aaattaaatc acaaattttt
    10501 gtattataaa taaccgcata agccaaatta tttgagatta ataaaacatc accaacttca
    10561 atatcaataa gacttctgta acatgtttta ctaaatccaa ttacaaaatg aatggaccaa
    10621 tataatgatt ttttgtctaa cgctggagcg tttccgtatt gacagactgt gactttatct
    10681 aaccaaagat tcaattcccc tgtccatatt cttattagtg gttgagcagg gctcaatact
    10741 ccatcaaata ctttacctac gcgcaaacga ttatatttta gttcaggatg ctttagattt
    10801 aacggggaac ttgataaact gagccattcc cctattaacc tttccgtttc tggcattaaa
    10861 gccatcccag cgagctcaga tgaaattaag ggaaatattt ctccaagcaa tacccatcca
    10921 gaccagctgc cactctcttc tgctatcggt agccatttag cactaaattc gggcattaat
    10981 aatgcaacat cttcgccatt ttctttccaa ttttgaaaag tggtttcaaa catatgagtg
    11041 tttcgattta ctttcctcag accgaacatt aactttcatc cttctgatcg ttttcgaatt
    11101 tgctttcatt ttcttgctcc tcatacggct gatgtcgttg tccttgtcgt tcatcttgct
    11161 cagtcaatac ccaacgttgt gctgtaacat cattgtgttg ggcttcacgg agtctgttgg
    11221 agacaagtgt gtctgatggt tttagtcgta tcccttcact actttccaga atccggactg
    11281 tatgattttg tccccaacgc tgaaactgat atgtcaattc attttcgtgc acattggcat
    11341 tattaacaga attatttata tcattctgat tgagcgtatt aacattcctt tgcttttcga
    11401 tatgttgagt aaacatggtt ctgttatcag caaaccttct ttcgttagta ctgtctaatg
    11461 aatgggcctg cggtccattt attttgtctc catcgcggta ttttttttca cgatctttat
    11521 tattactgtt ttttgatctg tgatgtattc tgtatggata ccattatgga ttttttcatt
    11581 aatttgtatc gttttattag atagttcaga actaataccc tgatctcgag aagtcatatt
    11641 gagaattttc tttgtagaaa attgttctgt tccatttgtt tgcaaagaaa gagaggaatt
    11701 cctgtcaacc tggagagatg atttgttgtc aatagacttc atttgcccat tctttttccc
    11761 caatgtagaa aattggctat cctgagtctg acgtaataac tggagaatgg ttccagcgac
    11821 aatctcactc atttctttat ttttttgttt ttctttaaga aactgtctaa attcgaagca
    11881 gaattatcgg gtgtgtctgt gtttaaaatt ttaccgtgtt caattttctt gacttcgtcc
    11941 attatatttt tcctcctgtt caagctcgtc ttgatgaagc atctgattta aaagtttttt
    12001 tgatgcgcgt ttttagtcga acgtactttt ctccttttag ccaccagtgt tttctttgtg
    12061 cttgctgttg aactatttcc ttgttaagta tttttctccg ctctgctatt ttttgctttt
    12121 caagatttat ctcagcgatc tgctgctgaa tgactgcaac tttacgtaac gagtaaaata
    12181 tatcctgtcg aaatacaact tcgccgagta actccttctt atctaacagt gacttcaggt
    12241 ttttaagttg catatcatac aggcgttcct tttcggctaa cttttctttt tctgcattta
    12301 acttttgtaa tgatgcttca catgcagcca ggctctgcgc tgttcttctg ataagtcgat
    12361 taactttact taacaattcg ttcaagctct tttagagttt cactcattgg cattttttca
    12421 ttaactgact gtttcaacca atttgtgagt ttaggccgtg cattcatagc gaaatcattt
    12481 tcagcatttt gcccagcgcg atattcccct aagtcaataa aaacctgtag atcttccaaa
    12541 gttgtgagga ttttccttac ccgtgcagca ttatctctgt gtttttcatc agtgacctga
    12601 ccaaatactc gactcacact ttttagaaca tttattgccg gatagtgacc ttgcccagcg
    12661 agtttgcgac tcagatagat atggccatcg aggattgagc gaatttcatc tcccaggggg
    12721 tctgattcgt cttccccttc cagtaacacg gtataaaacg cggtgatact tccttttagt
    12781 gttggtcccg gcctctccag taagcgaggc aaactgtcaa aaacagatgc aggataacca
    12841 cctctggcag gtggttcacc cgctgccagt ttcatatcac gtagtgctca tgcataccgc
    12901 gtcattgagt cgataaaaag tacaactctc tttcctcgat ctctaaaata ctctgccacg
    12961 gttgtcgcca tcagggcggc attacaccga tctactgacg agaaatcaga ggtggcataa
    13021 accaatacgc attttgccgc atttactgat tgtttcatcg attccgcgca ttccgtatct
    13081 tccctaccac gttcgccaat caagccaata acaaacacat cagcttcagt attgtttacg
    13141 agcatatgca ttaaaaccgt tttgccacag ccagccgatg caaaaatccc aacacgttga
    13201 ccaatgccac atgtcagtac gccatcaatg actcttatgc gtgtctctag tggtgtatta
    13261 ataactcgac gttcctgata cgacggtggc agttcatcaa tcaggcgtaa atttcctcta
    13321 tcgcctggag ggttcgggac aaacctttcc ataatttgac cagaggcatt caaaaccgaa
    13381 caaagaaagg catcactgat ctgtatggtt aactgctctc ccgttggttt caaaactgca
    13441 gtacgtgtaa gccccactga gctgcctata aggtttaata atgtctttcc attgcgaaag
    13501 ccaacgacct gagccctggc tatacgttca tttgcttgcc agcttgcata aatttcgcat
    13561 acttcaccca ctgaaacgtc gtcaagttct gcttcaatca gagagccgtt tatgctatgt
    13621 aagtagctgt atttatttaa taattttaat cttttcatac cggcatgctc catatcgcaa
    13681 gacattatcc taaatagttt tcagaatatt aacgggaaca ttttccgtta gttccccgta
    13741 cgaaagtact tcgagatctc gataactaga ttcaataagt tttttgataa aacgacgaat
    13801 atccacagat cccagtaaaa taatgtcttt aataggaata ttgattccag aaagtgccag
    13861 tttaaaatta tccagaatca tctcagcttc ggatgcatcg agattaagaa aagtaccgcc
    13921 agcggtaggc ctgacaccat ctctgattct gtcctctatt tcaggcgaaa tcacaattgc
    13981 tttaatttct ccagaatatg aaaacttatg gcaaatgtag cgacctaatg cacctcgtac
    14041 atgctcgacg agtgtgatta tatctttctc tcgtgggctc cataatgcta aggcctccat
    14101 aactaaccgc atattgcgca ctgaaattcg ctccaggatc agacgctgaa ttacctctga
    14161 aatgcgctgc acggttatat atcttaatat ctcttttaat agatcaggat acttcatctc
    14221 taattgatca agaatatagt tacatccttg ttttcatttc cccgtaagaa ccacatttca
    14281 gggtccagta gggttaactt ctgtccttga agcaaaacat tcattggaag taacagccct
    14341 tcttcaatca tgtttcatct ccttattcca tattctcatt ctccataaac ttgctataaa
    14401 aacatgatta aaaattgatg attactattt ataaaataat gattttgtta tgagcgaatt
    14461 gctatcagcc acttacacta gtacgtcatc ctgtccatat acagatttgt aactgttgat
    14521 atgttttact taaacacacc tcgtatttct tataacactt gtttggtaac tattttcact
    14581 acaaatcata tttgttagct cttcattaac acactgcgag tgttttgtta ctccccgcat
    14641 atattttatt ctcttgttcg attctatttt tcttaatcga tcatataatt ttcttgttag
    14701 tcggatttac gaatcatgag acttataatg gaaggaatga gactaagcgg ccaggttaaa
    14761 cctgtaatta ttgtagcaaa caaccgatat gttacttttt cttccgagaa gataaatata
    14821 aaagtcgatg tcacaaccag acctatcagg taagctaaca gtaatttatt gatcactttt
    14881 acctcccctt tcgatttctc tttgagcggt ccttttaatc caggaccgcc tcattatgaa
    14941 aactatttat ttccctcatc attgataagg ctggacatct ctttcaacat gagagtcatt
    15001 gaagctttcg accaatttag cggaatgata gggtttggcg cttcatgcaa agttcccgac
    15061 ttgtggatat agttatagct ttcgggaagt gccatttcag gtacactcct gccatttgca
    15121 ccaatcatat tctcgcctgt tatttgggcc aatgatctat tcataaattg cactgagtca
    15181 ttcaaatact cttcttttct tgattcttta taaacaattg ctgctgattt tgcataccat
    15241 gagtcaaaaa accattctgc ttctgttgat ggaatgaagc tcttttctct tttcacatgt
    15301 gaattttgat cggtatcggt tttaatatca ttgaaccaaa aatttgccga ctgataatta
    15361 tccttctcgt atctttttat accatacgga cctgctagtt ttttaactat ttttagaacc
    15421 tgctcttttc ttctggtgtt tatctttgca agatttgcag gataaataac attaagtaaa
    15481 gccgcatcgg cttctctgta atgtttatct ttttccagat aacctgggga ttctccccct
    15541 aaatcaagct gtaacgttat tctttcataa cctttatcta tgaggtggtt caacctcgtt
    15601 gttgaaagag gttcatccag ttcattggct ttagcttcgc gtagtaaatc agagacaaaa
    15661 acagagtctt tcttcgagag taagttcgac aaacgttcca gtccagaggt caccagtgca
    15721 acggaagatg tgtttaatcg ggcatcttct tcccatgctc cggaatcttc cataacatag
    15781 aaattggctt tatccagata agcaatcagc agcgcaaccg attttaatcg gtcacctttt
    15841 tgccaatctt cagcgttaat tgtaccagtg tcgattgctt gtataaggag atcaagataa
    15901 agacctaacg catcattttg tttatggttc cacagctgcg gcttgccttc ttcctgcaca
    15961 tctgccatca cgggggagtt gctgtcaaat cggatatgca cagcattcat ttgcccggga
    16021 actccatcca gtcgtttggg gttgctaatg acgtcctgca tccgcttaat ctggtcgggc
    16081 gtagacatat aatcccacag ggtgagcaga accttttttg cggctacact atttccctga
    16141 tctgatacta atgccatata gccccataaa ctatccctca gccagacagc atcatagtgg
    16201 gtttctgccg ctgttttgtc atcttcgaaa gtagaagcaa gaataaatgt tccccagggt
    16261 ttctccgtca acgttaactt tcgtgtgttt tctgttaatg ttaacaaccc ttgaatttcg
    16321 tctttggtat agtggctctt tatttcattt tttaatttgg ctgagtgaat cagaagacga
    16381 taattagggt acttatctgg atttaacgat aaataacttt tatctgttgc tgcccccgtg
    16441 gaattaagaa tgttaaatac agattcactc gtcacttctg cccctgtcgc ctggcatgtg
    16501 ccgcacgaaa atataccagc gagcagaaga gttaatgcag atttcccttt cataaaatat
    16561 tccatcaatc gtttgttaaa agcgtagata accacttatt taacaacttg aaaataaggt
    16621 gttttacctg ggttgttaca aaaggattgc attgcgtaaa cgctttttat ttacaacaaa
    16681 atggggaaat attacggcga ttgcgaaaag tctaaagcga ttgaattaag ataatataga
    16741 aaatcagata cagcaaaaat ggcagattga tgaggtgaag ataaattgga gcgggcgaag
    16801 ggaatcgaac cctcgtatag agcttgggaa gctctcgttc taccattgaa ctacgcccgc
    16861 ttcgagatgc gtaaggcatt ataaacctta cgctctcctt agcaagtgcc gcgctgctga
    16921 ctgctgatta attcgccatc agcattttgg cttgctgccc tgcggcggca agtaacgtag
    16981 cggatcaatt gccgttgcac ggtaacgaat ctggaaatgc aggcgaacag atgccgcatc
    17041 cgtgctcccc atagtggcga ttttttgccc agccttcacg ctttgcccat tatttaccag
    17101 catcgtgtca ttatgggcgt aagccgtaat gtaatcttca ctgtgtttaa tcatgatgag
    17161 attaccgtag ccacgcagct ggttgcccac atacaccacc tttcctgcac ccgcggcgta
    17221 aataggtgta ccccgtggag ctgagatatc aatcccttta ttgccgccat ctgctgtcga
    17281 atacggcatg ataactttcc ctgtcgttgg ccataaccaa caacgttgcc ctactggcgg
    17341 ccaggatgat ttcggtaccg ctgatgacgg tgtaaccgat gcggttttgg tcgttgattt
    17401 ggcggttgat ttacgtgtaa tactgctact tttcgcccca cccagtttta gtttctgacc
    17461 aacttcaatg gtgtaagggg gggaaatgcc gttcagtcgc gccagttctt ttacgctggt
    17521 tcccgtggtg cgcgaaatac gatatagcgt atccccccgt ttcacggtgt aaacggagcc
    17581 ggaatacgtt cctgtatcgg atgatttgct acccgaacag cccgccaaaa gcagtccaac
    17641 cgataacaac atcacgatac ccagagattt tttattcagg cgtcccgcac tcaaaatctt
    17701 tcctcgctta aaaaacaaga cgccatatca tagcagccag cctcttgata cccaatctct
    17761 tgcttctgga aaaacgtggt aatagagaaa gaggttaatt tcatttggca aagggaagta
    17821 tcatcagcaa aatattcgct gtagaggtat cgaagttcct gactttttac cctatttccc
    17881 atcctgatgc gtaataatcc tgatttatca cattgatttt atcgccactc atattgatac
    17941 gtatcactgt ccctattcag agtcctgttt cgtatcatat ctgtgttttt ttaacgtcat
    18001 cacacttcct tcctccccta acgcttatcg tcgtttctgg cgtaaatctt gcctgcttag
    18061 actaaatctt tgccataaga accaaatgtt caaatcgagg ggatttctat ggaagcgcgg
    18121 gaagcaaccg ctacgggtga atcatgcatg cgcgtcgatg ccattgctaa ggtcaccggg
    18181 cgggcacgat atactgacga ttatgttatg gcgggcatgt gttatgcgaa atatgtacgt
    18241 agccctatcg cacatggtta tgccgtaagt attaatgatg aacaagccag aagtttaccg
    18301 ggcgtactgg cgatttttac ctgggaagat gtgcctgata tcccattcgc tacagctgga
    18361 catgcatgga cacttgacga aaacaagcgc gataccgccg atcgcgcact gctaactcgc
    18421 catgttcgtc atcatggtga cgccgttgcc atcgtcgtgg cccgcgatga actcacggca
    18481 gaaaaagcgg cgcaattggt cagcattgag tggcaagaat tacccgttat caccacgcca
    18541 gaagcggcgc tggcagagga cgctgcacca atccataacg gcggcaattt actgaaacaa
    18601 agcacgatgt cgacgggcaa tgtccaacaa acaatcgatg ccgccgacta ccagatacag
    18661 gggcactatc agacccccgt tattcaacat tgtcacatgg aaagcgtaac atcgctggcg
    18721 tggatggagg atgactcgcg aattaccatc gtttccagca cccagatccc gcacattgtt
    18781 cgccgcgtgg ttggtcaggc gctggatatt ccctggtcat gcgtacgagt catcaaacca
    18841 tttgtcggtg gcggttttgg taataaacag gatgtactgg aagagccaat ggcggcattc
    18901 ctgaccagca agcttggcgg cattccggtg aaagtttccc ttagccgtga agagtgtttc
    18961 ctcgcaaccc gtacccgcca cgcttttact attgacgggc aaatgggcgt gaaccgcgac
    19021 ggaacattga aaggttatag tctggatgtt ctgtctaaca ccggcgctta tgcatctcac
    19081 gggcactcca tcgcttctgc gggggggaat aaagtcgctt acctttatcc tcgttgtgcc
    19141 tacgcttaca gttcaaagac ctgctatacc aacctcccct cggctggtgc gatgcgtggt
    19201 tatggcgcgc cacaagtcgt atttgccgtt gagtctatgc ttgatgacgc cgcgacagcg
    19261 ttaggtattg atcctgttga aattcgttta cgcaacgccg cccgcgaagg agatgctaat
    19321 ccgctcacgg gcaaacgtat ttacagcgca gggttgccgg agtgtcttga aaaaggccgg
    19381 aaaatctttg aatgggaaaa acgccgtgca gaatgccaga accagcaagg caatttacgc
    19441 cgcggcgttg gcgtcgcctg ttttagctac acctctaata cctggcctgt cggcgtagaa
    19501 atagcaggcg cgcgccttct gatgaatcag gatggaacca tcaacgtgca aagcggcgcg
    19561 acggaaatcg gtcagggtgc cgacaccgtc ttctcgcaaa tggtggcaga aaccgtgggg
    19621 gttccggtca gcgacgttcg cgttatttca acacaagata ccgacgttac gccgttcgat
    19681 cccggcgcat ttgcctcgcg ccagagctat gttgccgcgc ctgcgctgcg cagtgcggca
    19741 ctgttattaa aagagaaaat catcgctcac gccgcagtca tgctacatca gtcagcgatg
    19801 aatctgaccc tgataaaagg ccatatcgtg ctggttgaac gaccggaaga gccgttaatg
    19861 tcgttaaaag atttggcgat ggacgctttc taccaccctg aacgcggcgg gcagctctct
    19921 gctgaaagct ccatcaaaac caccactaac ccaccggcgt tcggctgtac ctttgttgat
    19981 ctgacggtcg atattgcact gtgcaaagtc accatcaacc gcatcctcaa cgttcatgat
    20041 tcggggcata ttctaaatcc gctgctggca gaaggtcagg tacacggcgg aatgggaatg
    20101 ggcattggct gggcgctatt tgaagagatg atcatcgatg cgaaaagcgg cgtggtccgt
    20161 aaccccaatc tgctggatta caaaatgccg accatgccgg atctgccaca actggaaagc
    20221 gcgttcgtcg aaatcaatga gccgcaatcc gcatacggac ataagtcact gggtgagcca
    20281 ccaataattc ctgttgccgc agctattcgt aacgcggtga agatggctac cggtgttgca
    20341 atcaatacac tgccgctgac gccaaaacgg ttatatgaag agttccatct ggcaggattg
    20401 atttgaggat aacatcatgt ttgattttgc ttcttaccat cgcgcagcaa cccttgccga
    20461 tgccatcaac ctgctggttg acaacccgca ggccaaactg ctcgccggtg gcactgacgt
    20521 actgattcag ctccaccatc acaatgaccg ttatcgccat attgttgata ttcataatct
    20581 ggcggagctg cggggaatta cgctggcgga agatggctca ctacgtatcg gctctgcaac
    20641 gacatttacc cagctcattg aagatcctgt aactcaacgt catctcccgg cgttatgtgc
    20701 tgcggcctca tccattgccg gaccgcagat ccgtaacgtc gctacctacg gtggaaatat
    20761 ttgcaacggt gccaccagcg cagattctgc cacgccaacg ctaatttatg acgcaaagct
    20821 ggagatccac tccccacgcg gtgttcgttt cgtcccgatt aatggctttc acactgggcc
    20881 aggcaaagtg tctcttgagc atgacgaaat ccttgtcgcc tttcattttc cgccccagcc
    20941 gaaagaacac gtgggcagcg cgcattttaa atatgccatg cgcgacgcaa tggatatttc
    21001 aacgattggc tgtgccgcac attgccgact ggataacggc aatttcagcg aattacgcct
    21061 ggcatttggt gttgccgcgc caacgccgat tcgctgccaa catgccgaac agactgcaca
    21121 aaatgcgcca ttaaacctgc aaacgctgga agctatcagc gaatctgtcc tgcaagatgt
    21181 cgccccgcgt tcttcatggc gggccagtaa agagtttcgt ctgcatctca tccagacgat
    21241 gaccaaaaaa gtgattagcg aagccgtcgc cgcggcgggg ggaaaattgc aatgaatcac
    21301 agcgaaacaa ttaccatcga atgcaccatt aacgggatgc cttttcagct tcatgccgca
    21361 ccaggtacgc cgctctcgga attactccgc gaacaaggac tgctaagtgt caaacaaggg
    21421 tgctgcgtgg gtgaatgtgg tgcctgtacg gtgctggtcg acggcacagc aatagacagt
    21481 tgcttatacc ttgccgcctg ggctgaagga aaagagatcc gcacgcttga aggtgaagcg
    21541 aaaggcggaa aactttctca tgttcagcag gcttatgcga aatccggcgc ggtgcagtgc
    21601 gggttttgta cgcctggcct gattatggct accacggcaa tgctggcgaa accacgcgag
    21661 aagccattaa ccattacgga aattcgtcgc ggactggcgg gaaatctttg tcgctgcacg
    21721 gggtatcaga tgattgtaaa tacggttctg gattgcgaga aaacgaagta aaaggatatc
    21781 cggcctgaat tcaggccgga ttcactgagg ttatgtgttt aacaactcat atttcttaat
    21841 cttgcgatag agcgtagcaa tgccgatgcc cagttcatca gcaacttgct tcttgctgtt
    21901 atgacgtgaa agcgcctcgc ggatcatttg cttttccatc tcctccagcg ccgtgccgcc
    21961 cgcatcatcg agtgacaggt gtgcctcagt gacctctgtt acatcacttt gctccgttgt
    22021 gccattattc agcagatttg gcggcaatag cgtgctgtcg ataacttcac ctgaaggaac
    22081 aacgttaacc agatattcca tcaaattgct taactcgcgc aggtttccgg gccaacgatg
    22141 cttacgcaat atttcgacga catcgggagc aatgccagga taaaccgatc ccagacgacg
    22201 ggtatgcaga tgtaaaaagt aatgcaccag tagttcaata tcttcctgac gttcacgcag
    22261 cggtggcaga gttatcggga taacattaag tcggtagaag agatcttcgc ggaatttacc
    22321 ttcggcaatg aactgggcca aattctggtt agttgcagaa atgatgcgaa tgtcgacttg
    22381 tattgggcta ctggcaccaa tcggcagaat ttcacgtgcc tcaatagcgc gcagtaattt
    22441 agcctgcaac attaatggca tatcacctat ttcatcgaga aacagcgtgc ccgtattcgc
    22501 cgcctgaatc aaccctgttt taccgttggc agaagcgcca gtaaatgcgc ctttaacata
    22561 accgaacagt tcgctctcca gaagctgctc cggaatcgcg gcacagttga tagcaataaa
    22621 gggtttattc cgtcttccgc tcaacttatg gattgcacgg gcgacaactt ctttacccgt
    22681 gccgctttca ccaaccacca taacgctgga tgggctgggt gcaatacggc taatgagtcg
    22741 ttttaattgc cgcataacac ggcactcgcc aaccaattgt tcaatatgtg gttcatcagg
    22801 tgcatttgct acagaaaaac tggtatgcga ttggtgaaat gccattaaaa ataattgtcg
    22861 gccctgaatg ttatgcaatt gaccaatgat taattcactt ttatcgtccc acgaaacaat
    22921 atgctgcata tgtccatgag taaaattact ctcaaatgtt aatggtctga aacggatagg
    22981 tttcccaata atattatttt gtacaacacc aagtgttttt aaggcagtct gattaacaaa
    23041 ctgaacccga ctttcatcat caacaactaa tacgccctga tccatattat cgatcatggt
    23101 cgcgaatatt ttactgatgt tatctcctgg cccctgatcc tccagaagtt tcgaaacaaa
    23161 aatggtggat atatggcgaa cataatcaga aaattcgcgt aaattatcac tgatatgctc
    23221 ttgttgctcg tgggtaacgg caatcaaact tatcacccca acacaacgat cctgtaaaat
    23281 gacaggcgta cccagaaatg ctttttcgcg gcaattttct ttactatcgc aaccttcgca
    23341 aaggggatcg aagcgagact gtgtcacaac tttttcagtt ttcgtttcca ggacgtggcg
    23401 gagcaggcgt gagttgccgc tcaactggcg accaagaaac ttcccatacg cgcccgttcc
    23461 ggcgacgcga cacaagtttt catcaacgat ctcaacctca agctgcaaaa cgctggcaag
    23521 cattctggca aaacgctgaa ttgtcggttg aatttgcatc aatactgact gcgtagtagc
    23581 aagctccata gctttacctt ccagacttac ttaaaagtcg atcattgaag acgttgatgg
    23641 ttcacagatc atgatgatat taactcaggc gaaattggct ttgataaaaa cataagattt
    23701 ttatcatttt ctaatgaaat tatggaagag atatcacatt tctatatcaa tatgagaatt
    23761 acggcggtga gtttatcaaa ctgaagagag atagcctgcc cctttatctt atttctgata
    23821 cttagcagca aataaataac gcgataaaaa aagccaaacg ttttcgtatt ttacaaacaa
    23881 ccagaagctg gcatcaattt gtgatcaacc ccacacatta tccgtcaaat tagtcttttg
    23941 cagccgcgcg gataattctg gcacacttat tgttagtccc aggtatagct gtgaaaacac
    24001 caatcacttt ggcaagtcac agtgaaataa accactttgc ctgtcattcc actaccggga
    24061 ctttatgatg aaaactgtta atgagctgat taaggatatc aattcgctga cctctcacct
    24121 tcacgagaaa gattttttgt taacgtggga acagacgcca gatgaactga aacaagtact
    24181 ggacgttgcc gcagcattaa aagcactgcg tgctgaaaac atctcaacca aagtctttaa
    24241 tagtggatta ggtatttccg tattccgcga caactccacc cgtacccgct tctcttatgc
    24301 ttccgcgctt aacctgctcg gccttgcaca acaagatctc gacgaaggca aatcacaaat
    24361 cgctcacggc gaaaccgtgc gtgaaaccgc caatatgatc tccttctgcg ccgacgctat
    24421 tggtattcgc gacgatatgt atctgggcgc aggcaacgcc tatatgcgtg aagttggcgc
    24481 tgcacttgat gacggttaca agcagggtgt actgccacag cgtccggctt tagtgaacct
    24541 gcaatgcgat attgaccacc cgactcagtc aatggctgac ctcgcgtggt tacgtgaaca
    24601 ctttggttca ctggaaaacc tgaaaggtaa aaaaatcgcc atgacctggg cctactctcc
    24661 aagctatggc aaaccgctct ctgtaccaca aggcatcatc ggtctgatga ctcgcttcgg
    24721 tatggatgtc accctggccc atccggaagg ctacgacctg atcccggatg tggttgaagt
    24781 ggcgaaaaac aatgctaaag cctccggtgg tagcttccgt caggtcacca gcatggaaga
    24841 agccttcaaa gacgcagaca tcgtttatcc gaagtcatgg gcaccttaca aagtgatgga
    24901 agagcgtact gaattgctgc gtgcgaacga ccacgaaggc ttaaaagcac tggaaaaaca
    24961 gtgtctggca cagaacgcgc aacacaaaga ctggcattgt actgaagaga tgatggaact
    25021 gacccgtgat ggcgaagccc tgtacatgca ctgcctgcca gctgatatca gcggcgtatc
    25081 ctgcaaagaa ggtgaagtga ctgaaggcgt attcgaaaaa taccgtatcg ctacctacaa
    25141 agaagccagc tggaagcctt atatcatcgc cgcgatgatc ctgtcccgta aatacgccaa
    25201 accaggtgca ctgctcgagc aactgctgaa agaagcgcaa gaacgcgtga aataacccct
    25261 cgggccgatc agctggtcgg ccctctattt ccagaggcca aaaggatagg atatgtccgt
    25321 tttctcattg aagattgata tcgccgataa caaatttttc aacggcgaaa catcaccgct
    25381 cttttcgcaa agccaggcca aactggcgcg ccagttccac cagaaaatag ctggttatcg
    25441 cccaacaccg ctttgtgcgc tggacgatct cgcaaacctt tttggtgtga agaaaattct
    25501 cgtcaaagac gaatcaaaac gattcggtct gaacgccttc aaaatgcttg gcggtgcgta
    25561 cgccatcgct caattattgt gtgaaaaata tcatcttgat atcgaaacgc tgtcatttga
    25621 gcacctgaaa aatgccatcg gcgaaaaaat gactttcgcg accaccaccg acggcaacca
    25681 cgggcgcggt gtggcgtggg cagcacagca actcggacag aatgcggtga tttacatgcc
    25741 gaaaggttct gctcaggaac gcgttgacgc cattctgaac ctcggtgccg agtgcatcgt
    25801 cacagatatg aactatgacg ataccgttcg cctgaccatg caacacgcgc agcagcacgg
    25861 ctgggaagtg gtacaggaca cggcatggga aggttacacc aaaatcccaa cctggatcat
    25921 gcaaggctac gcaaccctgg cagatgaagc cgtcgagcaa atgcgtgaaa tgggcgtaac
    25981 cccgacgcac gttctgctgc aagccggtgt cggtgcaatg gccggtggtg tgctgggtta
    26041 tctggtcgac gtctacagcc cgcaaaatct gcacagcatt attgttgaac ctgacaaagc
    26101 tgactgtatt tatcgctccg gcgtcaaagg cgacatcgtc aacgttggcg gtgatatggc
    26161 caccatcatg gcgggcctgg cctgtggcga acctaacccg ctgggctggg aaatcctacg
    26221 taactgcgcc acccaattca tctcctgcca ggacagcgtt gccgcattag gtatgcgcgt
    26281 gctgggtaat ccgtacggca acgacccgcg catcatctcc ggtgaatccg gcgctgtcgg
    26341 tttgggcgtt ctcgcagcgg ttcattatca cccgcaacgt caaagcctga tggaaaaact
    26401 ggcgctgaac aaagatgccg tagtgctggt tatcagtact gaaggcgaca ccgacgtgaa
    26461 gcactaccgc gaagttgtct gggaaggcaa acacgctgta gcaccttaat caccctattg
    26521 gaactactcc ctgcaaaacg gggagtaaaa aatctggaga aaaataatgg ctaagaatat
    26581 tccattcaaa ctgattcttg aaaaagcaaa agattaccag gcggacatga ctcgcttcct
    26641 gcgcgacatg gttgctattc ccagtgaaag ctgcgacgaa aaacgcgtag tacatcgtat
    26701 taaagaagag atggaaaaag tcggcttcga taaagttgaa atcgacccga tgggcaacgt
    26761 tctcggttat atcggccacg gcccgcgtct ggtggcaatg gacgctcata tcgataccgt
    26821 cggcattggc aacatcaaga actgggactt cgatccgtac gaaggcatgg aaactgatga
    26881 gctaatcggt ggtcgcggta cttccgacca ggaaggcggc atggcatcta tggtttatgc
    26941 cggtaaaatc attaaagacc tcggtctgga agatgaatat accctgctgg ttaccggtac
    27001 tgtgcaggaa gaagactgcg acggtctgtg ctggcagtac attattgaac aatccggcat
    27061 tcgcccggaa tttgtggtca gtaccgaacc aaccgactgc caggtatacc gtggtcagcg
    27121 cggtcgtatg gaaattcgta ttgatgttca gggtgttagc tgccacggtt ctgcaccaga
    27181 acgcggtgat aacgccattt tcaaaatggg tccgattctt ggcgaattac aagaactctc
    27241 ccaacgtctg ggttatgacg aattcctcgg caaaggcacc ctcaccgttt ctgaaatctt
    27301 cttcacatcc ccaagccgtt gcgctgtagc agacagctgc gcagtttcaa tcgaccgtcg
    27361 tctgacctgg ggcgaaacct gggaaggcgc gctggacgaa atccgcgccc tgcctgcagt
    27421 acagaaagct aacgcggttg tttctatgta caactacgac cgtccgtcct ggactggcct
    27481 ggtttaccca accgaatgct acttcccgac ctggaaagtg gaagaagatc acttcaccgt
    27541 taaagcactg gtgaatgcct acgaagggct gtttggcaaa gcgccggttg ttgataagtg
    27601 gaccttctca actaacggcg tatctatcat gggccgtcac ggcattccgg tgatcggctt
    27661 tggcccgggt aaagaacctg aagcgcatgc acctaacgaa aaaacctgga aatctcacct
    27721 ggtgacctgt gctgcgatgt acgctgcaat cccgttaagc tggctggcaa ccgaataatt
    27781 actcttcact tattcccctc cggttcgccg gagggttttt ggagtttgct atgcgcgtat
    27841 tgatcaaaaa cggcactgtc gttaacgcag atggacaagc caaacaggat ttgctgattg
    27901 aaagcgggat tgttcgccag ttgggcaaca atatttcgcc gcagctcccg tatgaagaaa
    27961 ttgatgccac tggctgttac gttttccctg gcggcgtgga tgtccatacg catttcaata
    28021 ttgatgtcgg catcgcg
//
LOCUS       DAMZET010000043        27374 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.043, whole genome shotgun sequence.
ACCESSION   DAMZET010000043 DAMZET010000000
VERSION     DAMZET010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.043"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            29..262
                     /locus_tag="POB34_001960"
     CDS             29..262
                     /locus_tag="POB34_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918911.1"
                     /translation="MAKTSGGAGFGALVGEELSITDTIETVVTGSVRANNGVLSPVME
                     KRMINLLIIVLRAVVAVANALIAVLELIRELID"
     gene            complement(327..503)
                     /locus_tag="POB34_001961"
     CDS             complement(327..503)
                     /locus_tag="POB34_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918912.1"
                     /translation="MNLVDAFVKKVISGPYKEYGKWWIDVEYISWGVPGKTRLMFESK
                     EQALEVKEGYKFLT"
     gene            895..1104
                     /locus_tag="POB34_001962"
     CDS             895..1104
                     /locus_tag="POB34_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HDI5918913.1"
                     /translation="MSMPPAIANTFLFEMMKSKSKDVTLAAIYALGEGRCQAENITRE
                     LHRLSQSDDMEIKIAAIKALGRIYR"
     gene            complement(1176..1838)
                     /gene="excA"
                     /locus_tag="POB34_001963"
     CDS             complement(1176..1838)
                     /gene="excA"
                     /locus_tag="POB34_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HDI5918914.1"
                     /translation="MKDKKETPDRFPTWWLLYYVLRKAYFFLGIPFFLFCALTSTLML
                     FSSRYYGDNIEDYVVTFGSWFLLLAPGIWMYSRAKTRREKIRKVVQTIKESGFYSPEK
                     GYEGLSLTQGAYFGIDLKNGTMLYVRIYPGNIMDVIGFDIHNFTRTVTDDKTLEIHTK
                     YINLPMVPIPSWCTHPETASNTMHAMASRGYDYPVDFPRLIQEKRKEWEQIAGMPVAE
                     VF"
     gene            complement(1903..4065)
                     /locus_tag="POB34_001964"
     CDS             complement(1903..4065)
                     /locus_tag="POB34_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HDI5918915.1"
                     /translation="MKILLRALCAGLAISSLPAMASVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLSTSAPTLIGSMFGAFNSIIAVLAVVWFMFIGIRHVVRSGHQGQVFST
                     GRDIVGTLSVVAGFLMIVPTGNGWSIAQLIMLWGASIMGVGSANVMVQLAADNIANGY
                     SMTVQPVQASTQTAARGIFEMELCKYAVNAGLNDFNQTAKSSTSLMTESAKTASGNYT
                     VTVSNGSGICGSASLSVEGNGTTDQSTIGKFFNPFSKNEYSGVISAQRAAMDNMISDM
                     DNAASEFVTTFLEKRNSGNGTLPDIETRIQRAADEYERAVQKSLPTDNGEQSRKEALK
                     SYLTTYGWVTLGAWYQTFATANQRLAELADRAPAVTSMSSLGEVGDTDLFSAVMSAYK
                     TQLQNTTYTPPLGTVTSANEQRMANAQDPKSALSEMMSFFVYRTNWIATEISGTGTTS
                     DQVNPLIKMKNIGDYTLGTTEILWSGYTTVRVLATVGDNSIFGKVANLFSGLPKGLVA
                     LLDAAAPPIYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCTAGPLWAA
                     THLGTSEDRGSRAAYGYIYLIDSMIRPPIMVFGFFFASVAIIAVGTILNALFGAALVN
                     VQFNSLTGIFSLAGFLLIYARICTTSVAAIFALQAYLPDHVINFLGGREGANTLGSMA
                     NSVKEIFIGGSRNIRHTPGMKTDRLKDKGSDDKDGIKG"
     gene            complement(4162..4746)
                     /gene="traX"
                     /locus_tag="POB34_001965"
     CDS             complement(4162..4746)
                     /gene="traX"
                     /locus_tag="POB34_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraX"
                     /protein_id="HDI5918916.1"
                     /translation="MNKLPENSDQTKNVKKSPSITRIATRGIINIFVPLMETRLITNS
                     VQHMLEQQKRRLDRFRQVNNKKAQLHLTWEEALQASHMSVDDLDRRFRRRRTVWRFCC
                     WSLLAIALFLSGMLFAASSLPLTTLVRAISTLVLILSGVALCASRALIVTYRLWQLHE
                     RKVSEPEQGTFRDFLNDRNGWRNATLIAVTSKQY"
     gene            complement(4775..5977)
                     /gene="traW"
                     /locus_tag="POB34_001966"
     CDS             complement(4775..5977)
                     /gene="traW"
                     /locus_tag="POB34_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraW"
                     /protein_id="HDI5918917.1"
                     /translation="MQRKTLLAALIATLSGTACQAHAYSVTVVASRPVEEQVIPRMEA
                     IKDVLGNILSTQTATGTAINQNSEKLASVIAQNGQATRQQMIFSNETQRLEEARKSFT
                     VPDSICSESASGIATESKSASASAASKLSKGGGVSNRSIRDRLASAANSPVREAYDGA
                     AIHASYCTEAEYARFGGTAVCPSVGEIPGGDSQVRSIYHGAGTADTPAALTWDQKQID
                     AATAYMKNTSRPSAGRALGKGEVNTQSGRTYVGLQNEYNGIIDSASNPQLTLIADSTP
                     NESTRKALAETLQSDSAAAYFDQVASPEAKARGYMSTREFEAFEAGRRYANTAYLVDL
                     QEMQGDNLLRELVRITAQMNWQLNDLKEQIRQGNVISGQQLALTARQYYEKQLGSLEK
                     TINQANAR"
     gene            complement(5944..6558)
                     /gene="traV"
                     /locus_tag="POB34_001967"
     CDS             complement(5944..6558)
                     /gene="traV"
                     /locus_tag="POB34_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraV"
                     /protein_id="HDI5918918.1"
                     /translation="MCTIHITPRCRRPARGQVFLKKLEVKGDSFSFTLPSRVFRLHPA
                     PNPRVIYASDARTQGKVTGMRLLLPSSTDELITTAIWQVSVPDEAPFDVQHVIQWILP
                     SPLASLMSDDTWLWPSVPGTPALSAGDWPVMDTDPSTLRRVRRRSFSIIDNRNRKGYI
                     TRRFQCFPLAPLPENRKYDSLPDLVLHPEQETIKCKEKHYSQPL"
     gene            complement(6558..9602)
                     /gene="traU"
                     /locus_tag="POB34_001968"
     CDS             complement(6558..9602)
                     /gene="traU"
                     /locus_tag="POB34_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraU"
                     /protein_id="HDI5918919.1"
                     /translation="MNINRLIFTIEDCLNTLSRFSVASSFVEYCDLRTVIGLDRQDRE
                     RRPWLNSPYIAATKRGEYLSVFEVSGAFREMDEASDQTGPGSLESLITSMSDSLNTAY
                     KNSGHKISFVFERDPDMGKEEIEDMVAPQKRSLANTGIQLQDVVDEKVTTLSPWLVRE
                     RCWLAIWSGPDLISNSDRTAHDELVRRLAERVPKARFAQSPWQWTLSALKIRHEAFLD
                     NVEQALRHSSDGLILRLLDIHEVGREIRRQTERHSTPRNWQPHLPEDAQPAGYRWTDD
                     ESVLHAPSLHLQLFNTQVTTQGNLVQAGGLWHGMVSITLPPQNLQTFNELVRAVPRAV
                     PWRIRMDLMPGGMKALNLKKTLLTYSSFISAVRPMYESVMALAATDEKEPVCIMTIMA
                     STWGKTREICTRNQAILKSAIEGWGVCGTTTTFGDPRRAWVNTILAASGGSGPVPLYP
                     PLSHAISLFPLNRAGSVWRGKGNLMLHTEDGSAIEVGLASSQQNKHTELAPGDPGLGK
                     SVLINTLSEIQISSAQKNLPFIAYIDKGYSAQGLVQLIRDSLPPERKDEAVGIILSND
                     PEYTRNLFDVMYGAKKPITPEKNFMSSVLCALCVDTGTGQPCNPGDTRQIINQLIELA
                     FKEYGENNPRLYRASTEELVDSALQDSGLYEKHDATWWARSTWFEVRDMLHNAGYIMA
                     AQRAHYQAMPQLPEVSSMLGHTSLRDVFGTVQRDGSNELLLDYIRRALEQGHNDYPMI
                     SGYTRFMINPETRVIAVDLNNVAGDKTPAGRLKTGIMYLLAGQIAGGDFTLPQYRDEV
                     LKQLPREYHEIALKRINQLDQEVKTKVYDELHNARGIDFIWENLDTQEREQRKFAIRT
                     VLSTQYLRDYPESVLKSANTLWLLRYKPEDIPVLRDNFNVPEFMLKRFLKMPEGPAPD
                     GSGVPVLGVFRVKSGTLARILKFTVGPLELWALNSSPKDSALRKTLTNKLGSVRARKI
                     LAENFPRGSATSLIEHRAGQHNSDNVIEELASELIRKQGYNL"
     gene            complement(9692..10492)
                     /gene="traT"
                     /locus_tag="POB34_001969"
     CDS             complement(9692..10492)
                     /gene="traT"
                     /locus_tag="POB34_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraT"
                     /protein_id="HDI5918920.1"
                     /translation="MQHSIKDLWLYPFPEIDVVHTQEPLLPEPELTTPGRCICCRQNV
                     RHRFRLDDSWPLRQLTDTISDTRVRLNKATEHLDKLKKRGEPVATGEKEKYNTAVKAA
                     ERALEQARLSARRLSLRHVQKAEITSTESLSEKEQELFHEDGPPYSLCAFCHAWHSLN
                     GYAAAQGVMVWLPDLHPSTVVALNRRSLQEVFSNDKFRVRRGREALSALMQNRLAVED
                     KFRSFRPADFADVFRRYPPSGRSPLREKMNGIALILTPDSFIKKEYVD"
     gene            complement(10476..10664)
                     /gene="traS"
                     /locus_tag="POB34_001970"
     CDS             complement(10476..10664)
                     /gene="traS"
                     /locus_tag="POB34_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative conjugal transfer protein TraS"
                     /protein_id="HDI5918921.1"
                     /translation="MSAWSPEHITLRRTENGNVIAHDSRDDQEFIFAECCSDEIANII
                     LEAVKRYTSTEPAHATQH"
     gene            complement(10728..11132)
                     /gene="traR"
                     /locus_tag="POB34_001971"
     CDS             complement(10728..11132)
                     /gene="traR"
                     /locus_tag="POB34_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraR"
                     /protein_id="HDI5918922.1"
                     /translation="MSRIRVVAARLYAAFIHAQLFVMSCRSKLTKFLLLIPMMLLPKA
                     VLADGDLADMVRNVEQGAKTAQSSSLTIAQFIGVIAFLGGLIGLKKVGKQGGMGLAGC
                     ILSIVIGALLAAGPEMMSRSQKQLGISSISIG"
     gene            complement(11183..11710)
                     /gene="traQ"
                     /locus_tag="POB34_001972"
     CDS             complement(11183..11710)
                     /gene="traQ"
                     /locus_tag="POB34_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HDI5918923.1"
                     /translation="MNMDALTAIENFASSIFSAGMDFLFTWGEFIGVISMITLFARAR
                     SAGPVKMSPGKFIAGMLTSCMLVSLPAMINAGGVQMGFRADSFGPIAYVQPQTFGAAA
                     GAANAVLSLAKLAGVGFVMNGISIWRKAGLDGHTALSASESVSKGNVKFIAGVLLVFI
                     DRVLNALLASIGIVF"
     gene            complement(11710..12414)
                     /gene="traP"
                     /locus_tag="POB34_001973"
     CDS             complement(11710..12414)
                     /gene="traP"
                     /locus_tag="POB34_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraP"
                     /protein_id="HDI5918924.1"
                     /translation="MKPETEIDETGSFGEPEEKPAPFWKRSVWGISVATWGLCAVVLL
                     AAIWYLFLRAPSETGMPPFNDADAGVQTWQTTQESSPSVQSTETMTVRTGEDMSQLAR
                     DVKTELDNRDEKIQATLNMLHDSINKLGEAIKKDEEYAQETRRQLDDIRSRLNGIMTQ
                     KSVTESSSTPHPAKKKTSSVLNGMKIMSMETGMAWIRWQGSTWAVREGQTLGNVVIQR
                     IDPTTRTIITSAGTLR"
     gene            complement(12414..13703)
                     /gene="traO"
                     /locus_tag="POB34_001974"
     CDS             complement(12414..13703)
                     /gene="traO"
                     /locus_tag="POB34_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HDI5918925.1"
                     /translation="MSAEQDAGKSGKKLAALLGLSSIILFGGSYIAFSKVSGSNSDMQ
                     SAVNINSAASGGTRSVTETPHYRELLRADNERGAAAAARNNQTFIASLPQGLDIPDTP
                     EKQQQPAAKPENYAYRQASGTPQEDRAASEKRMERLQKLIVRIKDQHPAGSTPTIATT
                     MWNKSPAETTGQNGTQQFALQNASLSTPVAEKGIQLIPALTRIPAYIDTAVDSDNPSS
                     KVIATIPAGPWAGATLFSPGVKLVGNGVEIHFDRMSWNGMDLKVNAYAQREDNLMSSV
                     ASNVNTRWFKHIILPSVLGGVGSIGTLYKDANTQVIQGNYGTVTGRVGMPSGEAVAGV
                     IAGGMAERGSQILTRQAESEPYKQVEVYQHEVVSILFVDPVMTNDARSSSLSSGISPS
                     VNRTSQAEQRSQARMQTAMEQRKAVMQRRYDEQPETP"
     gene            complement(13706..14689)
                     /gene="traN"
                     /locus_tag="POB34_001975"
     CDS             complement(13706..14689)
                     /gene="traN"
                     /locus_tag="POB34_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraN"
                     /protein_id="HDI5918926.1"
                     /translation="MQSRYLLSTLLLVCSAATSADNAGWQNARTPQTTNSASDHVQNA
                     SQNTGGVPATLVKGELPAPGQASPLVQDAARLDSELSADEIRSLRSLMADNERAINAP
                     ITSVVPRISSLTVNLSPGASLPLVRTAMNNLSVVTFTDINGSPWPQSDPPYNAAPKLF
                     DVQYNENMVTITPLRPWASGNISVYLKGLSVPVILNVTSGETDTPSSSQEMDSRLDLR
                     IPRQGPTSPVVSIPTDKIALHDATLQAFLDGIPPRDPSVKRLKFTGNVPDTTIWQHGD
                     DLLVRSRAILRDEFEQTLSSADGTHLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            complement(14700..15392)
                     /locus_tag="POB34_001976"
     CDS             complement(14700..15392)
                     /locus_tag="POB34_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HDI5918927.1"
                     /translation="MTENTELSSPSHPAPDPEILKSTIRAMKQSEQRANNVPALIKAL
                     LCTGTCLLISITGNAIQYWHSTNVEREYFATDNGRLVRLAPTNQPAWSQNDAMAFGSQ
                     ALATAFNLDFVHYRSQISSLSPRFSDEGFAGYVNALQASNILETIKKEKMNLTATTGA
                     GVLVRQGQMSDGVWFWTFQYPVRMRLVGQTTSKPEQSFVFEITIQRVDPRLKPSGMEI
                     RQMISRNAGPNS"
     gene            complement(15389..15736)
                     /locus_tag="POB34_001977"
     CDS             complement(15389..15736)
                     /locus_tag="POB34_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001629514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDI5918928.1"
                     /translation="MNMHKSLVAVLGLILISSTASAATCRAGSAAQAGSNTGYERARA
                     AADAWSQRENDVSSSLQSCLSRIKKISINLPQFPSLDDILSQLENQVCDAVVDKVNEK
                     LPGNIDPWKDYNL"
     gene            complement(15754..19521)
                     /locus_tag="POB34_001978"
     CDS             complement(15754..19521)
                     /locus_tag="POB34_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDI5918929.1"
                     /translation="MPSVSRYRTWLAVPADEIEDLKKAHPPMNGHTPVIWDKEHKLWF
                     ARPGADLSRLDRWLPRPQDVSMNGSDPVTEFAQVLENAGLVLKELPVMDGKIHRVPTA
                     DDKKGQKSGAYRGFLDGRPAGWYRDYRSADDSPITWTFSGGEQTDPRARLHLKAHSMQ
                     RREDAERELKAQYNRQAAYARRYVNKWPQATAHEYLTRKGIQAAPGVRVNNKNELVIP
                     FSNRNGAIRSYQRIPVTGGKDARILKDSEKTGNWFALGTPRNGQPVLFAEGYATAASL
                     HEATGLPVLMTVDGGNMIAVAENARQKWTQSPFIFCADNDHAIRVNKGIVSATKAAEL
                     TGGTVIFPAFTDAEKAQGLTDFNDLDASRGRAAFQHVINAQLEHIGVSTPNSNTPEIR
                     EALVIGNLVFTPVHTEEKTMTPTEYPETSPDTGHSHDQGPSLPSATQQEQPAASSSNI
                     ADETQSFTSHATENNGKDERHADNVQAQTESSVVQDSPAESPASPESTTASAPDEPAH
                     PAEPPEKVVSVATDKDWREFEAELSQPEKNESQQESGTSPEIPAPAAAPASPEDSPSS
                     ETMNESAEPSPSPDAVKEEMTVMTEPDDKQSTGISPDTPREEAVSHVTEDIPLQPEPF
                     LPESDGPEEYENYSAYQELMNNENPEHLQENSDMPSQPDTDHVQADESELQATTDTAE
                     PAVRDEPNPQQPVQSTSPSDNTSSFLDKARGFFTRKKTDSQAHENSDPTPSPETTTTT
                     PTPDSIVYAPERPDAPISLNLDEIIKSLKGEKRADRTVLYKLDGKPAFIDRVNRLEMV
                     DGASNDDRSVLAALAVATNFYGGVIELTGSDAFKQKAMRLIIEHNINVRMKHADQRLE
                     LDTLRKEMAVGKDAVVTHQPTPELNRNTPEQPVVPDPVQEKEATQSPASPVAPEASTV
                     STVPASSPAEPGKTADAAQGEEPPGKLRPGESVTAVLHNFGRAEYAPGKGESFFVELK
                     NRSGSKLYWGEQLESLVKNHQKGDVVTLTLQNREQFILPGEQKARFRNKWSMESVTNG
                     ISVSHDNPDKGQRIQAIPVETFMKVAAQISQGWPEEMKALRMPENVGSHLFIGEDRHP
                     VSAPQNANQVTEITSAAPDKLTPVLGSVDKDTRELNLLLVQSADEHLQGVVRLNGTLY
                     PALATPSADNSQLVINALTDKGLRFAGYGEAVNHDADSTNRPAPELMQFHLKTREEPL
                     FAAVYTPEKQPDALYRNLGFEQSWQQWSNSQKPEDRQEKTLHQDLSHSPGR"
     gene            complement(19611..20162)
                     /locus_tag="POB34_001979"
     CDS             complement(19611..20162)
                     /locus_tag="POB34_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HDI5918930.1"
                     /translation="MSGKKTIVTLLRVSLLACPLLFTTPSFAMIDTPSVKVGFSPEGS
                     ASALVLDTINSAESSIRMMAYSFTDPDVMHALAKAKKRGVDVRIVVDDKGNTNRASQE
                     AMKYINLLDIPLRTVDAFPIHHDKVIIVDGNTVETGSYNFSRAAARKNSENVVVLKNM
                     PDVAAQYLEHWQDRWNKGTDWKP"
     gene            complement(20177..20467)
                     /locus_tag="POB34_001980"
     CDS             complement(20177..20467)
                     /locus_tag="POB34_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDI5918931.1"
                     /translation="MKREGFWRYATPPVNIQGVPLPFTMIYLFICPFPSKTTFWICTG
                     IILFFVILDRSGWTVRTICTRIFSILRGAIASGRPWWYRHHTESPRDWTGLD"
     gene            complement(20464..21612)
                     /gene="traJ"
                     /locus_tag="POB34_001981"
     CDS             complement(20464..21612)
                     /gene="traJ"
                     /locus_tag="POB34_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HDI5918932.1"
                     /translation="MNIPDEFGLFPFQRFTADELRHFFVWCAAHKVSDVDLTGGSPVS
                     VSRFGRRVRCSSATLPTTLMSSLIDELFGREVIPRVLAGNPVDRTIQINGDASGRYGL
                     KRGERIRLRSHLIQGTSGAEEKAISITMRVIPTEIPDILSMNIEPDLLEAMVCKSGLG
                     FVCGETGSGKSTLCSALYRYIMDNFPDAKIVTYEDPVEYILGNENDLLPPHQAEIGRD
                     VVSFAAGLRSAVRRNPEIIGVGEIRDNETADAAVQAGNTGHYCLSTMHTKSPGETLAR
                     LLGLFPPVIRDSMAWAVLSLLQFILVQVLVRTNDGGRKAVREYIVINDELRDNLSGMP
                     HAEWGHHIDAIIRQEKRRIRDQILEMYIRNEVDRREAILFIPPGELRS"
     gene            complement(21609..22427)
                     /gene="traI"
                     /locus_tag="POB34_001982"
     CDS             complement(21609..22427)
                     /gene="traI"
                     /locus_tag="POB34_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraI"
                     /protein_id="HDI5918933.1"
                     /translation="MKALTSLLLPVILLTGCSSSPTISPGGVNTPPPDINAWLKPRSE
                     KPDSLSETRWKMLTDAGKTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISPEG
                     WLPPVIDEAQDVAHITPDQIRTSSRVWTIIRPERFVSNPPGWRDWLLRGLSTTATPGT
                     EGSVVPEDSVQRKVWETALRQGWQEGRQNADLTLEANQKTLTRDYRGMMLYSLLWRQG
                     MITRPDVSDQMQTVTGDGKKLVTGDRVRRLKNHAEFNLQKSHWRPLIGTEGGSR"
     gene            complement(22424..22882)
                     /gene="traH"
                     /locus_tag="POB34_001983"
     CDS             complement(22424..22882)
                     /gene="traH"
                     /locus_tag="POB34_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraH"
                     /protein_id="HDI5918934.1"
                     /translation="MNRIIPAGCLAALFLAGCNSPSQHTPVPRNYGAASDVAVTRHTQ
                     YTLWDKGVFSGQLPVSTGSHLLTANSDRLSFDWEGDAIELLNELARVRGMQFNYNGVR
                     LPLPVNLHVRDMTFSNTLRLIEAQTAWRATIHQYPGLLQVSFMQPENRKK"
     gene            complement(23277..23861)
                     /locus_tag="POB34_001984"
     CDS             complement(23277..23861)
                     /locus_tag="POB34_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDI5918935.1"
                     /translation="MMKPRSSYSKTAFILLFSVFLVAAVTKAKSSLPDITLEQAKEIN
                     ADNTVIFLFRHGERCDRSDMPCYSDKSGITITGTEKAQQEGIKFATIFSEYDIYSSNA
                     VRTIQTAKFFSGKEPVVMDSLSDCNNDLYKTLESIARESHKRNIVIMTHNHCLSFLAR
                     DRLGKKFKPAYLDALIMHYDGTRLILDGKYNKEA"
     gene            complement(23921..25123)
                     /locus_tag="POB34_001985"
     CDS             complement(23921..25123)
                     /locus_tag="POB34_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HDI5918936.1"
                     /translation="MMKKNHITRTIIASAVLFSFNAAAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKHNSNDDFSLLLPSVGAQVADPDDVSNKADDVKDDWDLFDSAVD
                     NQHGVQQAAANLKHRLQEFRNINADAQVGVSAVAAMANDTLPFALMVKSYGTVSVNGK
                     VNDADLDYLDKVANGTITDVDKNALTSRAFGRAAVITDVGISFAKELETAGQKWSLGV
                     TPKYQRVDLFNYNVTVRDYDKDDFDGDKYHNTKNGFNADIGAYTDLNDNWTVGLVAQN
                     IIPRSIDTKVVNGFKETFKVRPQATAGVSWHNDLFTTALDIDLTPASGFTSDSKRQFA
                     SVGAEFNAWKWAQLRAGYRQNMASNSGSAFTAGVGISPFDVVHIDVSGLVGTDHDYGA
                     MAQLQFTF"
     gene            complement(25208..26032)
                     /locus_tag="POB34_001986"
     CDS             complement(25208..26032)
                     /locus_tag="POB34_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDI5918937.1"
                     /translation="MRLNTTGIAARMMLSLDKKAIGEKLINGRQINPTPLQVRYPQGV
                     REVLGIMSEQLAISTADLTRILLEDALHNMFMPADNTTGNIISRIEYIMLSHDINAPL
                     LATLLSPWNIRSTVIQDPARLADYLSADALEHLAECFNLNPDWLNGHENYPIALSGEW
                     PDTADNFRMLISDSSNTEVIFWHSFPFAGNTKREYCGVILRQKKEINGSVIYPALSLS
                     PTLLNDEKRKWLTEYTTRQNTTMSLRRVTLRPGLAGNLITGQILPVSLFNTSLLPW"
     gene            complement(26183..27337)
                     /locus_tag="POB34_001987"
     CDS             complement(26183..27337)
                     /locus_tag="POB34_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDI5918938.1"
                     /translation="MPSPRIRKMSLSRALDKYLKTVSVHKKGHQQEFYRSNVIKRYPI
                     ALRNMDEITTVDIATYRDVRLAEINPRTGKPITGNTVRLELALLSSLFNIARVEWGTC
                     RTNPVELVRKPKVSSGRDRRLTSSEERRLSRYFREKNLMLYVIFHLALETAMRQGEIL
                     ALRWEHIDLRHGVAHLPETKNGHSRDVPLSRRARNFLQMMPVNLHGNVFDYTASGFKN
                     AWRIATQRLRIEDLHFHDLRHEAISRFFELGSLNVMEIAAISGHRSMNMLKRYTHLRA
                     WQLVSKLDARRRQTQKVAAWFVPYPAHITTIDEENGQKAHRIEIGDFDNLHVTATTKE
                     EAVHRASEVLLRTLAIAAQKGERVPSPGALPVNDPDYIMICPLNPGSTPL"
BASE COUNT         6365 a         6605 c         6967 g         7437 t
ORIGIN      
        1 gttcagcggg aagaacaggt tacaagtcct ggccaaaaca tcaggtggtg ctggttttgg
       61 tgctctggtc ggagaagaac tgtctataac tgacactata gaaaccgttg tgacaggcag
      121 tgtaagagcg aacaacgggg ttctttctcc agtgatggag aagcggatga ttaatctgtt
      181 aatcatcgtt ctcagggcag tagttgccgt tgcaaacgcg ctgattgctg ttctggaact
      241 gatccgggaa cttatcgact gataagcctg gaaacgtaac taagggcagg gagttaccgc
      301 ttcctgccct ttaacaaaaa gagatctcac gttaaaaatt tgtacccttc tttaacttcc
      361 agtgcctgct cctttgactc aaacatcagt cttgttttcc cgggaacgcc ccatgaaatg
      421 tattcaacat ctatccacca ttttccatat tctttatatg gcccggatat tacttttttg
      481 acaaaggcat ctaccagatt cattgttttt tcctttttgt gttgaaaata attaatgcca
      541 gagcacagcg aaaataatga atgcgctggc tgtgataatt gcgattttct cccatatgat
      601 atttttctca tgggggtttc tacggagatt atttcttcgc attgagatac cggtattcat
      661 gttttatctt ggacttttgt tttgttgtct gtctggcagg ctgttctgcg cccgtttgtg
      721 acaaggttag cttatggggt taaatggtca cggacatcat gcggacggtt ccctgtcaca
      781 tgccatctgg ttttttcatt caaattgaga gatattgcta agttgagtcg cttgcgatag
      841 tggactcctg tttgtaagtg agtgccattg tttatttcat gaagaggatt gattatgagc
      901 atgcctcctg cgatagctaa tactttcttg tttgaaatga tgaagagtaa atcaaaagac
      961 gttacacttg ctgcgatcta tgcattaggt gaaggccggt gtcaggcgga gaacattact
     1021 cgtgaattac atagattgag tcaaagtgat gatatggaaa taaaaattgc tgcgattaaa
     1081 gcacttggtc gaatttatcg gtaacggcat aagttatctg ttgtaatgaa caaaagccgg
     1141 ctcatttgaa ccggctgtta tggagggagc ttatcttaaa atacttcggc tacaggcatg
     1201 cctgcaatct gctcccactc cttacgcttc tcctgaatca ggcgaggaaa gtcgacaggg
     1261 taatcatatc ctcgacttgc catagcgtgc atggtgtttg atgcggtttc tggatgagta
     1321 caccatgatg gaataggaac cattggcagg ttgatgtatt tcgtatgaat ttccagtgtt
     1381 ttgtcgtctg ttacagtacg agtgaaatta tgaatgtcaa aaccgataac atccataatg
     1441 ttgcctggat agatacggac atacaacatt gtgccatttt taaggtcaat accgaaataa
     1501 gctccctgag ttaaagataa tccttcatac cccttctctg gagagtagaa gcctgactct
     1561 tttattgttt gtacaacttt tcttatcttt tctctccttg tttttgcacg tgaatacatc
     1621 catatacctg gcgcaagtaa taagaaccaa ctaccaaaag tgacaacata atcttcaatg
     1681 ttgtcaccat aataacgaga actaaataac atcagtgtag atgtcagtgc gcaaaataaa
     1741 aagaaaggta ttcctaaaaa gaagtaagcc ttccttaata cataatataa caaccaccaa
     1801 gttggaaatc tgtcaggtgt ctctttttta tctttcatac aatgcctccc ctcgtactat
     1861 ctattatttt aatgccgatg gggaggttgt gcaatattta tattatcctt ttattccgtc
     1921 cttgtcgtca cttcctttat ctttaagacg atctgttttc attccaggag tatgtcggat
     1981 attgcgactt cctccaataa atatttcttt aacagaattt gccatgctgc caagagtgtt
     2041 ggctccctca cgcccaccaa ggaaattaat aacatgatca ggtaggtagg cctgaagggc
     2101 aaatatggca gctacagagg ttgtacaaat tctggcgtat atcagcaaga aaccagcaag
     2161 actgaaaata ccggtcagcg aattgaactg tacgttaacc agggcagcgc caaataatgc
     2221 attcagtatt gtgcctacgg cgatgatggc aacagaagca aagaaaaaac caaagaccat
     2281 gatgggcggc cttatcatac tatcaattaa gtatatataa ccgtaggctg cccgacttcc
     2341 tctatcttct gatgttccga gatgtgttgc agcccacaga ggtccggcgg tacatcctat
     2401 taatacactg actatccagt tcccaatgcc tgtcatccag aaaatgaatg gtataaatgg
     2461 taggtaaatt gataaggaaa aacccgcaca aaacaacagg aacaacaaga aatatattgg
     2521 gggagctgca gcatccagca gagcaactaa tcctttaggt aatccagaga ataaattggc
     2581 aacctttccg aaaatagagt tatcacctac tgtcgcgaga actctaactg ttgtatatcc
     2641 actccagaga atctctgttg ttcctaatgt ataatcacca atatttttca tctttatcaa
     2701 tggattgacc tgatcgcttg ttgttcctgt gccacttatc tctgttgcta tccagttggt
     2761 tcgatagaca aagaagctca tcatttcaga taaggcagac ttaggatctt gcgcattggc
     2821 cattcgttgc tcattggctg atgtaacagt gcccagaggt ggtgtatagg tggtgttctg
     2881 taattgagtc ttgtaggcac tcatgactgc actgaataag tcagtatcac caacttctcc
     2941 cagtgatgac attgaagtta cagctggagc tctgtcagcc agctcggcca gacgctggtt
     3001 agcagtggca aaggtctgat accatgcgcc aagcgttacc cacccgtagg ttgtgagata
     3061 agatttaaga gcctcttttc tggattgctc tccgttatca gtgggtagcg atttttgtac
     3121 agcacgttca tactcgtcgg ctgctctctg aatccgtgtc tcgatatccg gcaatgttcc
     3181 gttgccagaa tttctttttt ccagaaatgt tgtcacaaac tcactggcag cgttgtccat
     3241 gtcgctaatc atattatcca tggctgcacg ttgtgctgag ataacaccgc tgtattcgtt
     3301 tttactgaag ggattaaaga attttccaat cgtcgattga tctgttgttc cgtttccttc
     3361 tactgacagg ctggctgaac cacagatacc gctgccattg gataccgtga cagtgtaatt
     3421 accactggcg gttttagctg attctgtcat cagtgaagtc gaggatttgg ccgtctgatt
     3481 aaaatcattt agtcctgcgt taaccgcata cttacacagt tccatttcaa aaatgccacg
     3541 cgctgctgtt tgtgtggatg cctgaactgg ctgtacagtc attgaataac catttgcgat
     3601 attatcagcg gcaagttgca ccatcacatt ggcggaccca acccccatta tcgatgcgcc
     3661 ccataacata atgagctgag cgatactcca gccatttccg gttggaacga tcatcagaaa
     3721 acctgcaacg acactcagtg tgccgacgat atcgcgtccg gtgctgaata cctgtccctg
     3781 atgccccgaa cgcacaacat gacggatacc aatgaacata aaccagacaa cggcaagtac
     3841 ggcgatgata ctgttgaacg ccccgaacat gcttccgatc agagtggggg cgctggttga
     3901 cagtggattg gtgactacat caccgaatat cgtgaccagc gcctggcgag acagatcgtc
     3961 aggattggtt gcggcagaca caatatcctg ataggtcact gaagccattg ccggcagtga
     4021 ggatatagca aggcctgcac agagtgcgcg aagtaaaatt ttcacagtaa ctccggaata
     4081 gaattgcgta tgggcaaagg ccacacagga tttaattaag ggggatggcg acatgccagt
     4141 ggtactggca tctggacgtg atcagtattg tttactggtt acggcaatta acgtggcatt
     4201 gcgccagcca ttgcggtcgt tcagaaaatc acggaaagtc ccctgctcgg gttcgctgac
     4261 ttttcgttca tgcaactgcc agaggcggta tgtaacaatc agtgctctgg aagcacaaag
     4321 agcgacgcct gacagtatca gcacaagagt gctgatggcg cgtaccagag tcgtaagagg
     4381 taatgaagag gcggcaaaga gcataccact gagaaacaaa gcgatggcca gcagactcca
     4441 gcaacagaat cgccatactg ttctgcgccg gcggaaacga cgatccagat cgtctactga
     4501 catatggctg gcctgcagag cttcttccca cgtgaggtgg agttgcgcct ttttattatt
     4561 tacctgacga aatctgtcca gtctgcgttt ttgttgttcc agcatatgct gtacagaatt
     4621 cgttatcagg cgagtctcca tcagaggaac aaaaatgtta ataatccccc gggtggctat
     4681 gcgggttatg gatggtgatt ttttgacgtt tttcgtctgg tctgaatttt ccggtaactt
     4741 attcattttt tcagtcctgt tctgacgtca gctttcaacg tgcgtttgcc tgattgattg
     4801 ttttctcaag gctaccgagt tgtttttcat aatactggcg ggcggtcaga gccagttgct
     4861 gtccagagat gacattaccc tggcggatct gttctttcag gtcgttcagt tgccagttca
     4921 tttgcgcagt gattcttacc agttcacgga gcaggttatc tccctgcatt tcctgtaaat
     4981 ccaccaggta tgctgtgttg gcgtagcgcc ggccagcctc aaaagcttca aactcacggg
     5041 tactcatata tccccgtgct tttgcttctg gtgaagccac ctggtcgaag taagcggccg
     5101 ctgagtctga ctgcagtgtt tcagccagtg ctttacgtgt gctttcatta ggcgtgctgt
     5161 cagcaatcag cgtcagttgg ggattcgacg cagaatcaat gatgccgttg tattcgttct
     5221 ggagtccgac gtaggttcgc ccactctggg tattcacttc tcctttaccc agagcacgac
     5281 cggcagaggg gcgggaagtg tttttcatgt aagccgtcgc tgcatctatt tgcttctgat
     5341 cccatgtcag tgctgcaggt gtgtccgctg tacctgcgcc gtggtaaatc gagcgtacct
     5401 gactgtctcc acctggtatt tcgcccacag aaggacaaac ggcggttcct ccaaaacgtg
     5461 catattctgc ttctgtacag taactggcgt gtatggccgc gccatcataa gcttcccgca
     5521 cgggagagtt tgctgcgctg gccagacggt cgcggatgct acggttgctc acaccgccac
     5581 ctttactgag ttttgaggct gcggatgctg aggcactttt gctttctgtt gcaatccctg
     5641 atgcggattc actgcagatg gagtctggca cggtgaagct tttccgtgcc tcctcgaggc
     5701 gctgagtttc attactgaaa atcatctgct gacgtgtcgc ctgtccgttc tgggcaatga
     5761 cacttgccag cttttcagag ttctgattga tagcggtacc cgtggctgtc tgggtactca
     5821 gtatgttgcc aaggacatct ttgatggctt ccattctggg gatgacctgc tcttcaacag
     5881 gccggctggc gacgacggtg actgaataag catgagcctg gcatgccgtt ccactcaggg
     5941 tggctataag ggctgcgagt aatgtttttc tttgcatttt attgtctcct gctcgggatg
     6001 gagcacaagg tcaggaaggc tgtcgtattt tctgttttca ggtagtgggg ccagtgggaa
     6061 acactgaaat cgcctggtga tataaccttt cctgttccgg ttatcgatga tgctgaaaga
     6121 acgacgtctg acgcgccgta aggtggaagg gtcagtatcc ataacaggcc agtcaccggc
     6181 actcagtgcc ggcgttccgg gaacggatgg ccataaccag gtatcatcgg acatcagcga
     6241 agccagcggt gaagggagta tccactggat gacatgctga acgtcgaatg gtgcttcatc
     6301 cgggacagaa acctgccata ttgccgttgt tatcagctca tctgtgctgg acggcaacaa
     6361 caaccgcatg cctgtaacct ttccctgtgt tctggcatcc gaggcgtata tcactctggg
     6421 attgggggcc ggatgtaacc tgaagacacg tgacggcaga gtgaatgaga aactgtcacc
     6481 ttttacctca agttttttaa gaaatacctg ccctctggca ggtctccggc agcgtggggt
     6541 gatatgaatt gtacacatca gagattgtat ccctgttttc ggatgagctc cgaggccagt
     6601 tcttcaataa cattgtcact gttatgttgg cccgcccggt gctcaattaa tgacgttgca
     6661 gaacctctgg ggaagttttc ggccagaatc ttacgtgccc gaactgaacc taacttattt
     6721 gtcagtgttt tacgcagggc actgtctttt ggcgatgagt tcagcgccca tagctccagt
     6781 ggaccgacgg taaatttgag tattctcgca agcgtgccac tttttaccct gaatactccc
     6841 agaacaggaa caccactacc atctggtgcc gggccttccg gcattttcag gaagcgtttc
     6901 agcataaatt caggaacgtt gaagttatcc ctcagtacgg ggatatcttc gggtttgtag
     6961 cgcagtaacc agagagtatt tgctgactta agaacgcttt ccgggtagtc cctcagatac
     7021 tgcgtactca gaaccgtgcg gatagcaaac ttgcgttgct cacgctcctg ggtatccaga
     7081 ttttcccaga tgaagtcaat gccccgggcg ttatgcagtt catcataaac ttttgtctta
     7141 acctcctgat caagttggtt aatacgttta agagcgattt catggtattc ccgtggaagc
     7201 tgctttaaca cttcatccct gtattgtggc agggtgaagt caccaccggc gatctgacct
     7261 gcaagcaggt acatgatgcc ggttttaagc ctgcctgcag gcgttttgtc accggcaaca
     7321 ttattcagat cgacagcgat gacccgggtt tccgggttga tcataaaccg ggtatacccg
     7381 gatatcatgg ggtagtcgtt atgtccctgc tccagggcgc ggcggatgta gtccagcaga
     7441 agctcgtttg aaccatcacg ctgaacagta ccgaaaacat ccctgagact ggtatggccc
     7501 agcatcgagg acacttcggg aagctggggc attgcctgat aatgtgcccg ctgagccgcc
     7561 atgatatacc cggcattgtg gagcatatcc cggacttcga accaggttga tctggcccac
     7621 caggttgcat catgtttttc atacagtccc gagtcctgca gtgctgaatc caccagttct
     7681 tccgttgagg cgcgataaag tcgcggatta ttttcaccgt actctttaaa agccagctcg
     7741 ataagctggt tgataatttg tcgggtgtct ccggggttac agggttgacc ggtaccggta
     7801 tcgacacaga gagcacacag caccgatgac atgaaattct tttccggcgt aataggcttt
     7861 ttagcgccgt acatcacgtc aaaaaggtta cgggtgtatt ccggatcatt gctcagaata
     7921 ataccgacag cttcgtcttt tctttccgga ggaagactgt cgcgaataag ctgaaccagt
     7981 ccctgagcac tgtatccctt atcgatatag gcaatgaagg gcaggttttt ctgagcagat
     8041 gatatctgta tttccgacaa tgtattaatc agaactgatt tacccagtcc cggatcgcct
     8101 ggtgcgagtt ctgtatgttt attttgttgg gatgacgcca gaccaacctc aattgcggaa
     8161 ccgtcttctg tatgtaacat cagattccct ttgcctcgcc agacagagcc tgccctgtta
     8221 agcgggaaaa gcgagatggc atgtgacaac gggggataca ggggaaccgg gccggagcct
     8281 ccggatgccg cgagaatggt attcacccag gcgcgtcggg gatcgccaaa tgttgtcgtg
     8341 gtgccacata cgccccagcc ttcaatggct gatttcagaa tggcctggtt tcgtgtgcat
     8401 atttcacgtg tttttcccca cgtggaggcc atgatggtca ttatgcagac aggttctttt
     8461 tcatccgttg ccgccagggc cataacggat tcatacattg gacgaacggc agaaatgaaa
     8521 ctgctgtaag tcagaagcgt tttcttcaga ttaagagcct tcatcccgcc gggcatcaga
     8581 tccattctga tgcgccaggg gactgcgcgt gggacggcac ggaccagttc gttaaacgtc
     8641 tgcagatttt gcggaggtaa agtaatcgat accattccgt gccagagtcc gcccgcctga
     8701 accaggttgc cctgtgtggt cacctgagtg ttgaataact gcaaatgcaa tgaaggagca
     8761 tgtagtacgg attcatcgtc agtccagcga tatcccgctg gctgggcatc ttcaggcagg
     8821 tggggttgcc agttacgcgg tgtgctgtgc cgttctgtct gacgacgtat ttcacgcccg
     8881 acctcgtgta tatccagtag ccggagaatc aggccatcag atgaatgtct cagtgcctgt
     8941 tccacattat caagaaaagc ttcgtggcgg attttcagtg cagataaggt ccactgccag
     9001 ggggactggg caaagcgtgc tttcggtacc cgttcagcca gtcttctgac aagctcatca
     9061 tgggcggtac gatcgctgtt gctgataagg tcgggtccac tccagatcgc cagccagcaa
     9121 cgctcccgga ccagccatgg ggacagtgtt gtgactttct catcgaccac atcctgtaac
     9181 tggatacctg tattagccag tgagcgcttt tgtggggcga ccatgtcttc gatttcttct
     9241 ttccccatgt caggatcacg ctcaaaaacg aagctgattt tatgaccgct gtttttatat
     9301 gccgtattca ggctgtcact catgctggtg atcagcgatt ccagtgaacc tgggccagtc
     9361 tggtcggatg cttcatccat ttccctgaaa gcaccgctga cttcaaagac agagagatat
     9421 tctccacgct ttgtggctgc aatatagggc gaattcagcc atgggcgacg ttcacgatcc
     9481 tgtctgtcca gacctattac agtacgtaaa tcgcaatact caacaaaact gcttgcgaca
     9541 gagaaacggg atagcgtgtt cagacagtct tcgatagtga agattaaacg gtttatgttc
     9601 ataggtgagg gaactccgga cttacaggag ggaggtttgt gcagtttgat ttttttaatg
     9661 ccaccccgcg ctggagtggc atgaagaaaa atcaatcaac gtattctttt ttgatgaatg
     9721 aatctggtgt cagaatcagg gcaatcccgt tcattttttc acgcaggggc gaacgaccgg
     9781 aaggtggata gcgacgaaaa acgtcagcga aatcggccgg gcggaaactg cggaatttat
     9841 cctcaaccgc cagtctgttt tgcatcagtg cagagagcgc ttcccgtccc cgccggaccc
     9901 ggaacttatc attactgaaa acttcctgta atgaacgacg gttaagggcc acgaccgtgg
     9961 atggatgaag gtcgggtagc cacaccataa cgccctgtgc tgcggcataa ccattcagtg
    10021 aatgccaggc gtggcagaaa gcacataagc tgtaaggcgg gccgtcttca tgaaacagtt
    10081 cctgctcttt ctctgacaac gactctgtac tggtgatttc agctttctgt acatggcgga
    10141 gtgaaagacg tctggcagac agacgggcct gctcaagcgc ccgttcagcg gcttttactg
    10201 ccgtgttgta cttttccttt tcgccggttg cgactggttc accacgcttt tttaacttat
    10261 ccagatgttc tgtggcctta ttaagacgaa ctcgtgtgtc actgattgtg tcggtaagct
    10321 gtctgagtgg ccatgagtca tccagtctga aacggtgcct gacgttttgc cggcagcaga
    10381 tacatcgacc gggcgttgtt aattccggtt ccggtaacag tggctcctgt gtatgaacca
    10441 catcaatttc tggaaaggga tacaaccaca agtctttaat gctgtgttgc atgagcaggc
    10501 tccgtactgg tgtaacgttt cacggcttcc agaatgatgt ttgcgatttc atcggaacag
    10561 cattctgcaa aaatgaattc ctgatcgtcc cgactgtcgt gggcaatcac attgccgttt
    10621 tctgttcgtc gtaacgttat gtgctccggg ctccaggcag acatatgtgc ctccttgtat
    10681 tgccgaacag gaagggcacc ggataaatgc cagtgccctg gacaggatta gccaatggaa
    10741 attgaggaaa tccccaactg tttctggctg cgactcatca tttcaggtcc ggcggcaagt
    10801 agcgccccaa tgacgataga tagaatgcaa ccagccagtc ccatcccccc ttgtttccca
    10861 acttttttca gcccaatcag gcccccgaga aacgcaatta cgccgatgaa ctgcgcaata
    10921 gtcagtgatg atgactgtgc tgttttggca ccttgttcga cattgcgaac catatcggca
    10981 agatctccat cggcaagaac ggctttgggc agcaacatca tgggaatgag cagcaggaac
    11041 ttagtcagtt tgctccggca tgacattaca aacagttgtg cgtggataaa tgcggcgtac
    11101 agacgggccg ctacgacacg aatacgtgac atagtttctc cttaaatgga tatatcagtg
    11161 gtttatatga aggtgaaaaa aatcagaaga cgattccaat gcttgccagc agggcattga
    11221 ggacacgatc gataaagacc agcaggacgc cggcaataaa tttgacgttt cccttactga
    11281 cggattcact ggcactcagt gcggtatgtc catcgagtcc ggccttgcgc cagatggata
    11341 tcccgttcat tacaaatcca acaccggcca gcttggccag cgacagaact gcatttgctg
    11401 cgccagcagc agcgccaaac gtctgaggtt gtacatatgc aatcgggcca aatgagtcag
    11461 ccctgaatcc catttgtacg cctccggcgt taatcattgc cgggagtgat acgagcatgc
    11521 agctggttaa catccccgca ataaattttc cgggagacat ttttaccgga ccggctgaac
    11581 gggcgcgggc aaacagggta atcatgctga tcaccccgat gaattctccc caggtaaaca
    11641 ggaaatccat tccggcactg aaaatactgc tggcaaagtt ctcaatggcg gtcagggcat
    11701 ccatgttcat cagcgtaacg tccctgcact ggtgatgatg gtccgggtag tcggatcaat
    11761 ccgctggatc accacattgc ccagcgtctg accttctctg acagcccagg tactaccctg
    11821 ccagcgaatc caggccatac cagtttccat cgacatgatt ttcattccgt taagtacaga
    11881 cgacgttttt ttctttgctg gatgcggtgt ggaagaggat tcagtgacgc ttttctgtgt
    11941 cattattccg ttcaggcggc tccggatatc atccagctga cggcgtgttt cctgtgcgta
    12001 ttcttcatcc ttttttatag cttcgcccag cttgttaatg ctgtcgtgaa gcatattcag
    12061 tgttgcctga attttttcat cgcggttatc cagctctgtt tttacatccc gggcaagctg
    12121 gctcatatct tctcctgtcc ggacagtcat agtctcagtg gactgaacgg aaggtgatga
    12181 ttcttgtgtg gtttgccagg tctgtacgcc agcatccgca tcattaaacg gaggcatgcc
    12241 tgtttccgat ggcgctctca ggaacagata ccatatggct gcgagcaaca cgactgcaca
    12301 tagcccccat gttgctacag atatccccca gacggaacgt ttccagaagg gggcgggttt
    12361 ctcttcgggt tcaccgaagc tgccggtttc atctatttct gtttcaggtt tcattatggt
    12421 gtctccggtt gttcatcata ccgacgttgc atcacagctt ttctttgctc catggctgtc
    12481 tgcattcgtg cctgcgagcg ttgttccgcc tgtgatgtac gattaactga aggggaaatg
    12541 ccactggaaa gggaagatga cctggcgtcg tttgtcatta ccgggtcaac aaacagaatt
    12601 gatacgactt catgctgata cacttctacc tgtttatagg gctcactttc tgcctggcgg
    12661 gttaatatct gacttcctct ttccgccata ccaccggcaa taacgcccgc aacagcttca
    12721 ccactgggca ttcccacgcg tccggtaacc gtgccatagt taccctgaat aacctgcgtg
    12781 ttggcatctt tataaagtgt gccaatactg cccacacctc ctaacactga cggcagaatg
    12841 atatgtttaa accagcgtgt attcacattg ctggccacgg atgacatcag gttgtcttcc
    12901 cgttgagcat atgcattgac cttaaggtcc atgccgttcc agctcatccg gtcgaaatgg
    12961 atctccacgc cgttacccac cagtttgact cccggactga acagcgttgc ccctgcccag
    13021 gggccagcgg gaatggttgc gataaccttg gatgacgggt tatcagaatc cactgcagta
    13081 tcaatatatg ccggtatacg ggtcagggca ggtatcagtt gtatcccttt ttctgctaca
    13141 ggcgtggaca ggctggcatt ctgtaatgcg aattgctgcg taccattctg ccctgttgtt
    13201 tctgccggag atttattcca catagtcgtt gctatggttg gtgtactacc ggcaggatgc
    13261 tgatctttta ttcttacaat cagcttttgc agccgttcca tacgcttttc actggctgct
    13321 cgatcctcct gtggtgtgcc ggaagcctgg cgataagcat agttttccgg ttttgctgca
    13381 ggctgttgtt gcttttccgg agtgtccgga atatcaagcc cctgcggaag actggcaatg
    13441 aacgtctgat tattgcgggc tgccgccgca gcccctcgtt cattatccgc tcttaacagt
    13501 tcacgatagt gaggcgtttc ggtaacgctg cgcgtaccgc cacttgcagc actgtttatg
    13561 ttcacggctg actgcatatc gctgttactc cctgatactt tggaaaacgc gatatagctg
    13621 ccgccaaata agatgatgct gctcagccca agcagggcag ccagtttctt tccggattta
    13681 ccggcatcct gttcagcact catgattact ccagttctgg tgtcacgtga acggactggc
    13741 cattcacaga gaaagtcagt agtggggtga cgggaagctt ccagagatga gttccgtcgg
    13801 cagaggacaa ggtctgttca aattcatcgc gcaatatcgc ccgggaacga accagaaggt
    13861 catcgccgtg ttgccagatc gttgtatccg ggacattacc tgtgaatttc agcctcttaa
    13921 ctgaagggtc ccggggagga ataccatcca gaaatgcctg taatgttgca tcatgcaggg
    13981 cgattttatc cgtcgggatg gagacaaccg ggctggttgg tccctgccgt ggtattcgca
    14041 gatccagacg actgtccatt tcctgactgg atgacggcgt atctgtttcg ccactggtta
    14101 cgttcaggat gaccggtacc gacagacctt tcaggtagac agaaatgttt ccggatgccc
    14161 atggccgcaa tggtgtgatt gtcaccatgt tctcgttgta ctgcacatca aagagttttg
    14221 gtgccgcgtt ataaggagga tcggattgtg gccatggtga accgttgata tcggtaaatg
    14281 tcactacgct caggttgttc atggctgtgc gaacaagagg aaggctggca ccgggggaca
    14341 gattaactgt cagagagctg atacggggta caacactggt gatgggcgca ttgatggccc
    14401 gctcgttatc cgccatgaga ctgcgtagtg agcgaatctc atcggcggag agctcagaat
    14461 caagtcttgc cgcatcctgt accagcgggc tggcctgccc gggggcaggc agctcacctt
    14521 ttaccagtgt tgccggaaca cctccggtgt tttgggaggc gttctgaacg tgatcgctgg
    14581 cagaatttgt cgtttgtggt gtgcgtgcgt tctgccatcc tgcgttatcg gcagacgtgg
    14641 cggcgctgca caccaggagc agtgtgctca gtaaataacg tgattgcata ttacttatct
    14701 caggagttcg gtccggcgtt acgggagatc atctgccgga tttccatacc ggatggtttc
    14761 agtctggggt caacacgctg gatggtgatt tcgaaaacaa aagactgttc tggcttgctg
    14821 gttgtctggc cgaccagacg catacggaca ggatactgaa atgtccagaa ccatacgccg
    14881 tcggacattt gtccctgtcg taccagaacg ccagcgcctg tcgtggcggt cagattcatc
    14941 ttctctttct tgatcgtctc cagaatgtta gatgcctgta atgcattcac atatccggca
    15001 aaaccttcgt cagaaaaacg cggagacagg gaggaaatct gtgaacgata gtgcacaaaa
    15061 tccaggttga atgcggtcgc cagtgcctgg ctgccaaagg ccattgcgtc attctgactc
    15121 catgctggct gatttgttgg tgccagcctg accagacgac cattgtctgt agcgaaatat
    15181 tcgcgctcca cattcgttga gtgccagtac tgaatggcgt tgccagtaat actgataaga
    15241 agacatgtgc ccgtacataa aagcgctttt atcagcgcag ggacattatt tgcccgctgt
    15301 tctgactgct tcatagccct gattgtgctc ttcagtattt ccgggtcagg tgccggatgt
    15361 gatggcgagg atagctcggt attttcggtc ataggttgta gtccttccag ggatcaatat
    15421 tccccggcag cttttcattc accttgtcca cgaccgcgtc gcagacctgg ttttccagct
    15481 gcgacagaat gtcgtcaagg ctggggaact gggggaggtt tatgctgatt tttttgattc
    15541 gtgacagaca ggactgcagg gatgaggaaa catcgttttc acgttgtgac catgcatccg
    15601 cagctgcacg tgctcgctca tatcctgtat tactaccggc ctgtgccgcg ctacccgcac
    15661 gacaggttgc agcacttgcc gtggatgaaa tcaggataag gccgagcacg gcaaccagag
    15721 acttatgcat gttcatatgg cttccttacc ggctcatcgg ccggggctgt gggagagatc
    15781 ctgatgcaga gttttttcct gcctgtcctc cggcttttgg ctgttggacc actgctgcca
    15841 tgactgctcg aagcccaggt tccggtacaa cgcatcaggt tgtttttccg gcgtgtagac
    15901 agcagcaaaa agcggttctt cccgggtttt cagatgaaac tgcataagct ccggtgcggg
    15961 gcgattcgtg ctgtcagcat cgtggtttac cgcttcacca taacctgcaa agcgtaaacc
    16021 tttgtcggtc agcgcattga taaccaactg actgttgtcg gccgatgggg tggccagtgc
    16081 cggatacaat gtgccgttca gccggaccac accctgcaga tgttcatcgg cactctggac
    16141 aagtagcagg ttgagttcac gcgtatcctt atccactgat cccagaacag gtgtcagctt
    16201 gtcaggggct gcgctggtta tttctgttac ctgatttgcg ttctgtggcg cggagaccgg
    16261 atgtcggtct tcaccgataa acagatgtga gccgacgttt tctggcatac gtaacgcttt
    16321 catctcttcg ggccacccct ggctgatttg cgcagccact ttcataaagg tttcgaccgg
    16381 aatcgcctga atacgctgac ctttatccgg attatcatgt gataccgaga tcccgttggt
    16441 aactgactcc atggaccact tattccggaa acgtgctttt tgctcaccag ggagaatgaa
    16501 ttgttcgcgg ttctgaagtg tcagtgtcac cacgtcacct ttctgatggt ttttgaccag
    16561 actttccagt tgttcccccc agtagagttt gctgccactg cggtttttaa gctcaacaaa
    16621 gaaactctct cctttgccgg gtgcatattc agcacgacca aaattgtgca gtacggcagt
    16681 cacgctctct cccgggcgaa gttttcctgg tggttcttca ccttgtgccg catctgccgt
    16741 tttgccaggt tcagccggtg atgatgcagg gacggtgctg acggttgatg cttcaggggc
    16801 aactggggac gccggggatt gtgtggcttc tttttcctga acggggtcag ggacaaccgg
    16861 ttgttcaggg gtattccggt taagttccgg cgttggctga tgggtgacaa cagcatcctt
    16921 accgacagcc atttccttac gtaatgtatc gagctccaga cgctgatcag cgtgtttcat
    16981 gcgcacattg atgttgtgct cgataatcaa ccgcatcgct ttttgcttaa aggcatcact
    17041 gcctgtcagc tcaatgacgc caccatagaa attagtggcc acagcaagtg cggccagaac
    17101 actgcgatcg tcattgctgg caccgtcgac catttccagc cggtttaccc ggtcaatgaa
    17161 ggctggtttg ccatccagct tgtacaggac ggttctgtct gctcgttttt cacccttcag
    17221 tgattttatg atttcgtcca gattaagcga tatcggtgca tcaggacgtt ccggggcgta
    17281 gacaatactg tcaggtgtcg gtgtggtcgt cgtggtttcc ggtgagggcg tgggatcact
    17341 gttctcatgt gcctgtgaat ccgttttttt acgcgtaaaa aagccacgcg ctttgtcgag
    17401 aaacgatgaa gtattatcag aaggtgatgt gctctgaaca ggttgctgtg gattgggttc
    17461 atcccggaca gccggttctg cagtatctgt tgtggcctga agttctgact catctgcctg
    17521 aacgtggtct gtgtccggtt gcgacggcat atcactgttt tcctgcagat gctcaggatt
    17581 ttcattgttc atcagctcct gatatgcgct gtagttttcg tattcttcgg ggccatctga
    17641 ctcaggaagg aacggctctg gctgaagggg aatgtcttca gttacatggc tgaccgcctc
    17701 ttcccgggga gtatccgggg atattccggt gctttgtttg tcatcaggct ccgtcatcac
    17761 ggtcatttcc tctttgactg catcagggga aggtgatggc tcggctgatt cattcatggt
    17821 ttcagatgag ggactgtctt caggggatgc gggggcggca gcaggggccg gtatttcagg
    17881 tgaagtgccg gattcctgtt gtgactcgtt tttttccggt tgtgacagtt cggcttcaaa
    17941 ttcacgccag tctttatctg tggccaccga gacaactttt tccggaggtt ccgccggatg
    18001 agcgggttca tcaggggcag atgccgttgt actttccggg gacgccggtg attctgcagg
    18061 ggagtcctga actacagagc tttccgtttg cgcctgaacg ttatcagcgt gccgctcatc
    18121 tttgccgtta ttttcagtgg cgtgactggt aaatgactgt gtttcgtcag caatgttact
    18181 gctggaagca gctggttgtt cctgctgcgt ggcggacggc agagaaggtc cctggtcatg
    18241 gctgtgccct gtatcgggtg atgtttcagg atattctgtc ggagtcatgg ttttttcctc
    18301 agtgtggacc ggtgtaaaaa cgaggttgcc gatcaccagt gcctcgcgga tttccggggt
    18361 gttactgttg ggggtggaaa ccccgatgtg ttctaactgg gcgttgatga catgctgaaa
    18421 agcggcccgt ccccggcttg cgtccaggtc attgaaatcg gtcagcccct gcgctttttc
    18481 ggcatcggtg aacgcaggaa agatgaccgt tcctcctgtc agttctgcgg cttttgttgc
    18541 actgacgata cctttgttaa cccggattgc atgatcgttg tcggcacaga agataaaggg
    18601 gctttgcgtc cacttttgcc gggcattttc ggcaacggca atcatgttgc ctccatccac
    18661 cgtcatcagt accggaaggc ccgtggcttc gtgcagggag gccgcagtgg catagccctc
    18721 ggcaaacagt accggctgac cgttgcgcgg tgtgcccagc gcaaaccagt taccggtttt
    18781 ctctgaatct ttcaggatgc gggcatcctt ccccccggtg accggaatac gctggtaaga
    18841 gcgtatcgcg ccgttacggt tactgaaggg aataaccagt tcatttttgt tgtttacccg
    18901 cacccccggt gcagcctgaa tgcctttccg ggtcaggtat tcatgtgctg tggcctgcgg
    18961 ccatttgttg acatagcggc gggcatacgc ggcctgtcgg ttgtactgtg ctttcagttc
    19021 ccgctccgcg tcctccctgc gttgcattga atgcgctttc aggtggagtc ttgcgcgggg
    19081 atctgtctgt tctccgccgg agaaggtcca ggtgatcggc gaatcatccg cgctgcgata
    19141 atccctgtac cagccggcag gtctgccgtc caggaatccg cgatatgcgc cacttttttg
    19201 tccttttttg tcatcggcag tcgggacacg gtggattttc ccgtccatga ccggcagttc
    19261 tttcagaacc agtccggcat tctccagaac ctgggcaaac tcggtgaccg gatcactgcc
    19321 gttcatggag acgtcctgtg gccggggcag ccagcggtcc agcctggaca ggtccgcccc
    19381 cgggcgggcg aaccagagtt tgtgctcctt gtcccagatg acgggcgtgt gcccgttcat
    19441 cgggggatgt gcttttttga gatcttctat ttcatctgca ggcacggcaa gccaggtgcg
    19501 gtagcgggat acagacggca tgagtgcttc tcctgaaaaa agacggaaaa aaaccaccgc
    19561 cggaagccgg gggtggctgg ggggatagaa aatcggggga gaaatgtttg tcagggtttc
    19621 cagtctgtac ccttattcca gcgatcctgc cagtgttcaa gatattgtgc ggctacatcc
    19681 ggcatattct tgagcacaac gacattctct gaattcttgc gtgcagcagc acgagagaag
    19741 ttataggagc cagtttcaac cgtgtttccg tcaacaatga tgactttgtc gtgatggatg
    19801 gggaaggcat ctacagtccg gagaggaatg tccagcaggt tgatatattt catcgcttcc
    19861 tgacttgccc ggttcgtatt ccccttgtca tcaacaacaa tacggacgtc cactccgcgt
    19921 tttttcgctt ttgccagcgc atgcattaca tccgggtcgg tgaaggaata tgccatcatc
    19981 ctgatggagc tttccgcact gttgattgtg tcaagcacca gagcggatgc gcttccttca
    20041 ggagaaaagc cgaccttcac ggatggcgtg tcgatcattg caaacgatgg tgtcgtgaac
    20101 agtaacgggc atgccagtag cgatacccgg agcagagtaa cgatagtttt tttacctgac
    20161 ataaagactc cagaatttaa tcgaggccag tccagtctcg tggtgattcc gtatggtggc
    20221 gataccacca tggacgaccg gaggcgatgg cccctctcag aatgctgaaa atgcgtgtac
    20281 agatagtcct gacagtccag cctgaacggt cgagaataac gaaaaataaa atgatgccgg
    20341 tgcaaatcca gaaggttgtt ttggagggaa aggggcatat aaacagataa atcatggtga
    20401 acggcagagg tacgccctgg atgttgactg gcggtgttgc atatcgccag aaaccttctc
    20461 ttttcatgac cgtaactccc ctggcgggat aaaaagaatc gcttctcttc ggtcaacttc
    20521 attccggata tacatttcca gaatctggtc ccggatacgg cgcttctcct gccggattat
    20581 ggcatcaatg tgatgccccc actctgcatg cggcattccg gaaagattgt cgcgaagctc
    20641 atcgttaatg acgatatatt cccggacggc tttacgacca ccgtcatttg ttctgaccag
    20701 aacctgaacc agaatgaact gcaacagtga caggacagcc caggccatcg aatccctgat
    20761 gacaggggga aacagcccaa gaagacgtgc cagcgtttca ccaggagatt tcgtatgcat
    20821 ggtgctcagg cagtaatgcc cggtgttgcc tgcctgtacc gctgcgtcgg cggtttcgtt
    20881 atccctgatc tcaccaacac cgataatttc gggattacgt cgtacagcag aacgcagtcc
    20941 ggcagcaaaa ctgaccacat cacgcccgat ttctgcctga tgcggtggga gcagatcgtt
    21001 ttcatttccc aggatatatt ccaccgggtc ttcatatgtc acgattttgg cgtcggggaa
    21061 attgtccata atgtaacggt acagtgctga acacagcgtt gattttcctg acccggtttc
    21121 accacataca aatcccagac cgcttttgca gaccattgct tcaagcaggt ctggttcaat
    21181 gttcatggag agaatgtccg gtatttctgt cgggatgaca cgcatggtta ttgagattgc
    21241 tttctcctcc gcaccagatg tgccctgaat gaggtgagag cgtaaccgga tgcgttctcc
    21301 gcgtttaaga ccatagcgcc cggatgcatc cccgttaatc tgtatcgtcc tgtcaaccgg
    21361 attgcctgcc agaacgcggg gaatgacttc ccgaccgaac agttcatcaa tcagggagga
    21421 catgagtgtg gttggtaacg ttgcactgga acaacgcacc cgtcggccaa agcgactgac
    21481 agatacaggt gacccgccgg tcagatccac atcactgact ttatgtgcgg cacaccagac
    21541 aaagaaatgc cgtagttcat ctgcagtgaa acgctgaaac ggaaaaagac caaactcatc
    21601 aggaatattc atcgggagcc tccttcagtg cctataagcg gacgccagtg agatttctga
    21661 agattgaatt ccgcatgatt tttaaggcga cgaacacgat ctccagtgac cagttttttc
    21721 ccgtcacctg taacggtttg catttgatca gaaacatccg gacgggtgat catgccctga
    21781 cgccagagaa gtgagtacag catcatgccc cggtaatcac gggtgagggt tttctggttg
    21841 gcttcaagcg tcaggtcggc gttttgtctt ccttcctgcc atccctgccg taatgcggtt
    21901 tcccacactt ttcgttgcac gctgtcttct ggaacgacgc tgccttctgt acccggagtg
    21961 gctgttgttg aaagcccccg gagaagccag tcacgccagc ccggtggatt gctgacaaag
    22021 cgttcagggc gaatgatggt ccagacccgg gaagaggtcc ggatttgatc tggcgtgata
    22081 tgagctacgt cctgtgcttc atcaataacc ggtggtagcc agccttccgg gctaatcagt
    22141 ggccggaaat cgtataaggc attaagtgtg ctttcccggg cgttgagcgc ctgaatcagc
    22201 tcccatgaac gctgagcctt gccaccacga aatccaagcg ttttacctgc gtctgtcagc
    22261 attttccagc gtgtttctga cagactatcc ggcttttcac ttcgtggttt aagccatgca
    22321 ttaatgtcag gtggtggcgt attgacacca cctggcgaga tggtagggga agaagaacaa
    22381 ccagtaagca gtattactgg caaaaggaga gaggtgagtg ctttcatttt ttccggttct
    22441 ccggctgcat gaagctgacc tgtaacaagc ccggatattg atggattgtt gctctccagg
    22501 ctgtctgggc ttcaataagt ctcaacgtat tggaaaacgt catatcccgg acgtgaaggt
    22561 tgactggtaa cggcaggcgg acaccattgt agttaaactg catccccctg actctggcca
    22621 gttcgttcag taattcaatt gcatcacctt cccagtcaaa ggaaaggcga tcgctgttag
    22681 ctgtcagaag atgagacccc gttgaaaccg gaagttggcc ggaaaataca cctttgtccc
    22741 ataatgtgta ctgagtgtgc cgggtaaccg ccacatcaga ggcagcaccg tagttacgcg
    22801 gaacaggagt atgctgagaa ggggagttgc agccagccag aaaaagggcg gcaagacacc
    22861 ccgcaggtat aatacggttc ataataattc ctgagcagtt aaatcagaat gggatatacc
    22921 ggtgctccgg tacagacagg atgatgtttg tttccggcta tacttgcgtt accggaaata
    22981 aatgccagtg tggccaggta aatagcctca tagtttactg aaggtgatta ttcctgatac
    23041 tggctggttg atgtgtcgtc agacataaaa cgaatatgag cgtaatcttt aacgctccgc
    23101 gccgaatcga tccatgatgg atgactcgac gatttatgaa tcgaacgtac cggcgcttgt
    23161 ccctgaaaca gttcccggcc ctcgtccgga taacctgcaa ttcagacagg cacatgtgcc
    23221 cgtacactct tgtatcttac tgagtatatc agaatttatg taaattcaaa tatcctctat
    23281 gcctctttgt tgtattttcc gtcaagtatg agccgggtac catcgtaatg catgatcagg
    23341 gcatcaaggt acgccggttt gaatttcttg ccgagcctat ctctggccag aaatgataaa
    23401 caatgattat gtgtcattat tactatgttc cttttatgag attctcttgc gattgattca
    23461 agcgtcttat aaagatcatt attgcagtct gataaactgt ccataacgac gggttcttta
    23521 ccggagaaga attttgcagt ttgtatggtt cttaccgcat tgctggagta aatatcatat
    23581 tcactgaaga ttgttgcgaa cttaatacct tcctgctgtg ctttctctgt accggtaata
    23641 gtgattccgc ttttatccga atagcatggc atatctgaac ggtcgcaacg ttctccatgg
    23701 cggaaaagga aaataacggt gttatcagca ttaatttctt ttgcctgttc cagggtgata
    23761 tcgggaagcg agctttttgc ttttgtcact gccgctacca ggaaaacaga aaataaaaga
    23821 atgaatgctg tttttgaata agaagaacgt ggtttcatca tgttatttaa actgaatata
    23881 aaaatacccc cgggtttagt gccgggggcg gggacagtaa tcagaaagtg aactgaagtt
    23941 gtgccattgc accgtaatcg tggtcagttc ctaccaggcc ggaaacatcg atatgtacga
    24001 cgtcaaatgg agagatacca acgccagcgg tgaaggcgct tccggagttt gatgccatgt
    24061 tctgacgata accggcacgc aattgcgccc atttccatgc gttaaattca gcaccaacac
    24121 ttgcaaactg acgtttgctg tcagaagtga agccactggc aggagtcaga tcaatgtcaa
    24181 gagcagtggt aaacaggtca ttatgccagg atacaccggc ggttgcctgc ggacgaactt
    24241 tgaaggtttc tttaaagcca ttgacgactt tagtatcaat actgcgtgga atgatgttct
    24301 gggcaacaag accgacagtc cagttgtcat tcaggtctgt ataagcacca atatcggcgt
    24361 tgaatccgtt tttcgtgttg tggtacttgt caccgtcaaa atcgtctttg tcgtaatcac
    24421 gaaccgttac gttgtagtta aacaggtcca cacgctgata ttttggtgtg acgccaagag
    24481 accatttttg tccggccgtt tccagttctt tggcaaatga aattccgacg tcggtaatta
    24541 cagctgcacg cccgaatgcg cgtgaggtca gggcattctt atcgacgtcg gtaattgttc
    24601 cattggcaac tttgtccaga taatccaggt ctgcatcgtt tacttttccg tttacgctga
    24661 cagttccgta agacttgacc ataagggcaa acggcagagt gtcatttgcc atggctgcta
    24721 cagctgatac acctacctga gcatctgcat tgatattgcg gaattcctgc agacgatgtt
    24781 taagatttgc cgctgcctgt tgtacgccat gctggttatc tacagcactg tcaaacaaat
    24841 cccagtcatc tttaacatca tcagctttat ttgatacatc atcaggatca gcaacttgtg
    24901 ccccaacaga cggcagaaga aggctgaaat catcattgct gttgtgcttt gtcagtaaag
    24961 ccgggtttgc cagaggtgcc acaccataat gggaagatgc taccccggta ccacccattg
    25021 catcgttacg cgcttcaaaa tatgacgtcg ctgctgcagc gttaaaggaa aataatacag
    25081 cagatgcaat gatggtgcgg gtaatatgat ttttcttcat catttaattt cctgtttatc
    25141 agaatttaga caaaacaacg ctatgacacg gatgtgccgt aatttttact ttggtactat
    25201 ctggtattta ccagggtaat aaagacgtat taaataagct aacaggtagt atttgccctg
    25261 taataaggtt cccggcaagg ccaggtctca acgtgacgcg tcgcaaagac attgttgtat
    25321 tctgcctggt tgtatattca gttaaccatt ttcttttttc atcgttcaga agtgttggtg
    25381 ataatgatag tgccggatat attacagagc catttatttc ttttttctgt ctcaatatta
    25441 ccccgcaata ttctcttttc gtatttcctg cgaaggggaa actatgccag aaaataactt
    25501 cagtgttgct gctatcacta ataagcattc tgaaattatc agcagtatct ggccattcac
    25561 cggacaacgc tatcggatag ttttcatggc cattcagcca gtccggattg agattaaaac
    25621 attcagcaag atgttcgaga gcgtcagctg aaagatagtc tgcaagtctg gccgggtcct
    25681 gtataaccgt ggagcggata ttccatggcg acagcaatgt tgccagtaat ggcgcattta
    25741 tatcatgcga cagcatgatg tattcaattc tgcttattat gtttcctgtg gtgttatccg
    25801 caggcataaa catattatga agagcatcct caagcaggat tcgggttaaa tcagcggtgg
    25861 atatggctaa ttgttcgctc attatcccca gaacttctct taccccttgg gggtaacgga
    25921 cctgcagagg tgtcgggttt atctgacgac cgttgatgag tttttcgccg attgcttttt
    25981 tgtccagcga gagcatcatt cgggccgcaa taccggtcgt attcagtctc attgttcctc
    26041 cgactgaaaa agtcttttca gctcatgtgg ttattgtatc ttcggtgttt tttctggtga
    26101 aacgaataat ttcgatcatg cagatcgaat gtgtttggcg agttgttgca actcgccttt
    26161 ctgtatgtgt tttgaatgat gtttacagcg gggtgctgcc cgggttcaga gggcaaatca
    26221 taatgtagtc agggtcgtta acaggtaacg ctccgggaga tgggacacgt tcgcctttct
    26281 gtgctgcaat ggccagtgta cgcaacaaaa cctcactggc gcggtgaact gcttcctctt
    26341 tcgttgtggc agtgacgtga aggttatcaa aatcaccgat ctcaatacga tgcgctttct
    26401 gcccattttc ttcatcgatg gtcgtgatat gggcaggata cggcacaaac catgctgcta
    26461 ctttttgtgt ctgccgccgg cgggcatcaa gcttactgac cagttgccat gcacgaagat
    26521 gagtataccg tttcagcata ttcatggaac gatgtcctga tattgcagca atctccatta
    26581 cattcaggct acccagttcg aagaagcggc ttattgcttc atgccgtaga tcgtgaaaat
    26641 gcaggtcctc gatgcgaagt cgttgtgtgg ctattctcca ggcattttta aagccggatg
    26701 cggtgtaatc aaaaacattg ccgtggagat taacgggcat catctgaaga aagttacggg
    26761 cacgtctgga cagaggaaca tcccgtgagt gaccgttttt ggtttcaggt aaatgagcca
    26821 caccgtggcg caaatcaatg tgctcccaac gtaaggccag tatttcgccc tgccgcatgg
    26881 ctgtctcaag ggcaagatgg aaaatgacat acaacatcag atttttttcg cggaaatagc
    26941 gagaaaggcg acgttcttct gaagacgtta gccggcgatc tcgtcccgag gatactttcg
    27001 gcttgcgaac cagttcaacc gggttagtac gacaggttcc ccattcaaca cgagcaatat
    27061 tgaacagaga tgacagaagg gcgagttcaa gacgtacagt attacctgta atgggtttac
    27121 ccgttcgggg gttgatttct gctaaacgaa cgtctctgta tgtagcaata tcaacggttg
    27181 ttatttcgtc catattccga agggcaatgg gatatcgctt gataacattg ctccggtaaa
    27241 actcctgttg atgccctttc ttgtgaacag aaactgtttt caggtactta tccagtgcgc
    27301 gtgacaggga cattttacgg atgcgtggag acggcatgct aacctcaaaa gaggcaaaaa
    27361 ataatgccaa caat
//
LOCUS       DAMZET010000044        27196 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.044, whole genome shotgun sequence.
ACCESSION   DAMZET010000044 DAMZET010000000
VERSION     DAMZET010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.044"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            212..1255
                     /gene="nadA"
                     /locus_tag="POB34_001988"
     CDS             212..1255
                     /gene="nadA"
                     /locus_tag="POB34_001988"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDI5918939.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSISV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            1293..2012
                     /gene="pnuC"
                     /locus_tag="POB34_001989"
     CDS             1293..2012
                     /gene="pnuC"
                     /locus_tag="POB34_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HDI5918940.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(2009..2950)
                     /gene="zitB"
                     /locus_tag="POB34_001990"
     CDS             complement(2009..2950)
                     /gene="zitB"
                     /locus_tag="POB34_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HDI5918941.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIKMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(3064..3444)
                     /locus_tag="POB34_001991"
     CDS             complement(3064..3444)
                     /locus_tag="POB34_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HDI5918942.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            3760..4812
                     /gene="aroG"
                     /locus_tag="POB34_001992"
     CDS             3760..4812
                     /gene="aroG"
                     /locus_tag="POB34_001992"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HDI5918943.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(4970..5722)
                     /gene="gpmA"
                     /locus_tag="POB34_001993"
     CDS             complement(4970..5722)
                     /gene="gpmA"
                     /locus_tag="POB34_001993"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDI5918944.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(5927..6967)
                     /gene="galM"
                     /locus_tag="POB34_001994"
     CDS             complement(5927..6967)
                     /gene="galM"
                     /locus_tag="POB34_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HDI5918945.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            complement(6961..8109)
                     /gene="galK"
                     /locus_tag="POB34_001995"
     CDS             complement(6961..8109)
                     /gene="galK"
                     /locus_tag="POB34_001995"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDI5918946.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDNFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(8113..9158)
                     /gene="galT"
                     /locus_tag="POB34_001996"
                     /pseudo
     CDS             complement(8113..9158)
                     /gene="galT"
                     /locus_tag="POB34_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
     gene            complement(9168..10184)
                     /gene="galE"
                     /locus_tag="POB34_001997"
     CDS             complement(9168..10184)
                     /gene="galE"
                     /locus_tag="POB34_001997"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDI5918947.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(10445..11917)
                     /gene="modF"
                     /locus_tag="POB34_001998"
     CDS             complement(10445..11917)
                     /gene="modF"
                     /locus_tag="POB34_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HDI5918948.1"
                     /translation="MSSLQILQGAFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            complement(11985..12773)
                     /gene="modE"
                     /locus_tag="POB34_001999"
     CDS             complement(11985..12773)
                     /gene="modE"
                     /locus_tag="POB34_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HDI5918949.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VIMSLPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            12902..13051
                     /gene="acrZ"
                     /locus_tag="POB34_002000"
     CDS             12902..13051
                     /gene="acrZ"
                     /locus_tag="POB34_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HDI5918950.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            13218..13991
                     /gene="modA"
                     /locus_tag="POB34_002001"
     CDS             13218..13991
                     /gene="modA"
                     /locus_tag="POB34_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI5918951.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVAIFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            13991..14680
                     /gene="modB"
                     /locus_tag="POB34_002002"
     CDS             13991..14680
                     /gene="modB"
                     /locus_tag="POB34_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDI5918952.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            14683..15741
                     /gene="modC"
                     /locus_tag="POB34_002003"
     CDS             14683..15741
                     /gene="modC"
                     /locus_tag="POB34_002003"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HDI5918953.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(15742..16560)
                     /gene="ybhA"
                     /locus_tag="POB34_002004"
     CDS             complement(15742..16560)
                     /gene="ybhA"
                     /locus_tag="POB34_002004"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HDI5918954.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            16715..17710
                     /gene="pgl"
                     /locus_tag="POB34_002005"
     CDS             16715..17710
                     /gene="pgl"
                     /locus_tag="POB34_002005"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HDI5918955.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYSIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(17751..18704)
                     /locus_tag="POB34_002006"
     CDS             complement(17751..18704)
                     /locus_tag="POB34_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI5918956.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            18888..19940
                     /locus_tag="POB34_002007"
     CDS             18888..19940
                     /locus_tag="POB34_002007"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HDI5918957.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            20016..21449
                     /locus_tag="POB34_002008"
     CDS             20016..21449
                     /locus_tag="POB34_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDI5918958.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            21632..23893
                     /locus_tag="POB34_002009"
     CDS             21632..23893
                     /locus_tag="POB34_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HDI5918959.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAQKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYKIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(24127..25410)
                     /locus_tag="POB34_002010"
     CDS             complement(24127..25410)
                     /locus_tag="POB34_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HDI5918960.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(25545..26615)
                     /locus_tag="POB34_002011"
     CDS             complement(25545..26615)
                     /locus_tag="POB34_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI5918961.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTALHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(26593..26811)
                     /locus_tag="POB34_002012"
     CDS             complement(26593..26811)
                     /locus_tag="POB34_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDI5918962.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(26851..27018)
                     /locus_tag="POB34_002013"
     CDS             complement(26851..27018)
                     /locus_tag="POB34_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918963.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENISDCYDHWMLWAQIAHADVTNI
                     RIEELKEHQAA"
BASE COUNT         6466 a         6700 c         7014 g         7016 t
ORIGIN      
        1 caggcatcct caatttcact ttgtaaacct gatgacatcg tcagagctta ctgtgcaagc
       61 aactctacgt cggtggaatt aggcgtaatt cgagtgactt ttccccacca ttcgactatc
      121 ttgtttagca tataaaacaa attacaccga taacagcgaa tattacgcta atgtcggttt
      181 taacgttaag cctgtaaaac gagatggtaa gatgagcgta atgtttgatc cagacacggc
      241 gatttatcct ttccccccga agccgacgcc gttaagcatt gatgaaaaag cgtattaccg
      301 cgagaagata aaacgtctgc taaaagaacg taatgcggtg atggttgccc actactatac
      361 cgatcccgaa attcaacaac tggcagaaga aaccggtggc tgtatttctg attctctgga
      421 aatggcgcgc ttcggtgcaa agcatcccgc ttctactttg ttagtcgctg gggtgagatt
      481 tatgggagaa accgccaaaa ttctcagtcc ggaaaaaaca attctgatgc cgacacttca
      541 ggctgaatgt tcactggatc tcggctgccc tgttgaagaa tttaacgcat tttgcgatgc
      601 ccatcccgat cgtactgtcg tcgtctacgc caacacttct gctgcggtaa aagcgcgcgc
      661 agattgggtg gtaacttcaa gcatttccgt cgaacttatt gatcatcttg atagtttggg
      721 tgaaaaaatc atctgggcac ccgacaaaca tctggggcgt tacgtgcaaa aacagacggg
      781 tggagacatt ctatgctggc agggtgcctg tattgtgcat gatgaattta agactcaggc
      841 gttaacccgc ttgcaagaag aatacccgga tgctgccata ctggtgcatc cagaatcacc
      901 acaagctatt gtcgatatgg cggatgcggt cggttccacc agtcaactga tcgctgctgc
      961 gaaagcattg ccacatcagc ggcttatcgt ggcaaccgat cggggcattt tctacaaaat
     1021 gcagcaggca gtgccagata aagagttact ggaagcacca accgcaggtg agggtgcaac
     1081 ctgccgcagc tgcgcgcatt gtccgtggat ggccatgaat ggccttcagt ccatcgcaga
     1141 ggcattagaa caggaaggaa gcaatcacga ggttcatgtt gatgaaaggc tgcgagagag
     1201 ggcgctggtg ccgctcaatc gtatgctgga ttttgcggct acactacgtg gataacgaat
     1261 aataaggcgt aacgttacgc tttgggggaa agatggattt ttttagtgtg cagaatatcc
     1321 tggtacatat accaataggg gcaggcggtt atgatctctc atggatcgaa gcggtaggca
     1381 cgatcgccgg gttgctgtgt attggccttg ccagtctgga gaagatcagc aactacttct
     1441 ttggcctgat caacgtcacc ttgtttggca ttattttctt tcagattcag ctgtatgcca
     1501 gcctgctatt acaggtgttt ttctttgccg cgaatattta cggttggtat gcgtggtcgc
     1561 gacaaaccag tcagaacgag gcggagttga aaattcgctg gttgccattg ccgaaggcac
     1621 tcagctggtt ggcggtttgc gttgtttcga ttggtctgat gacggtattt atcaatccgg
     1681 tgtttgcatt tttgacccgc gtggcagtca tgatcatgca agcattagga ttacaggttg
     1741 tgatgcctga actgcaaccg gacgctttcc cgttctggga ttcatgcatg atggtgttat
     1801 ctatcgtggc aatgattctg atgacgcgta agtatgtgga aaactggctg ttgtgggtga
     1861 ttattaacgt gattagcgtc gttatttttg cacttcaggg cgtttacgcc atgtctctgg
     1921 agtacatcat cctgaccttt attgcgctca acggcagccg gatgtggatc aacagcgcac
     1981 gtgaaagagg ctcacgcgcg ctgtcccatt aatggtgatg atgtgaatgg ccagacaccc
     2041 cttcattcag gtggcagtct ggcccatgac aaggttgata ttccatctta atggtggcgt
     2101 gctcaatctg atagtgatcc atcaggtagt gttggatctg atccaacaag gcatcgtgat
     2161 cgtgtggcgg gatcacctgc acatgcaacg tcatcaccgg cttctcgcct accatccata
     2221 catgtacatg gtgcacattg cggacttccg ggatttcccg gcacatacga cgcttcagtt
     2281 cagcgatatc cagcgatacc ggtgcacctt caagtaattc attcacgcta tctttcaaca
     2341 gtcgccacgc gctacgcaga accagaagcg acaccagtat cgagagaatg gggtcagcag
     2401 gtgtccagcc agtccagata ataatcaacg cggcaataat agccccaacc gaacccagca
     2461 ggtccccgag cacatgcagt gccgctgctc gtacgttgag gtttttctct tcgctgccgt
     2521 gatgaagtaa ccagaaagaa agtatatttg ccagcaaccc agccacggca attgccatca
     2581 tcatgccgcc ctcgaccgga cgcggcgtgc ggaagcgttc tatcgcctcc cagacaatca
     2641 aaatggtaat taccacgagg gctatggcat tcacaaacgc cgcgagcgtg gtcagtctta
     2701 gccaaccgaa agtgtggcga atggtgggag gacgacgtga aaattgcacg gcgagtaggg
     2761 cgaaaagcag ggcggcagta tcggtcaaca tatgacccgc atcggccagc aatgccagag
     2821 aaccagaaag aaaaccacca acgacttcta ccagcataaa cccggcagtc acgccgaaag
     2881 catacaacaa gcgacgagca ttattatctt ctggcaggtg tgaggacgtg tgtgagtgtg
     2941 agtgcgccat gacgagtcat ccctttatta ttctcactta actttatgac atcatactgc
     3001 ttttagaagt gaaaaattaa aagggagaga ctccgctctc ccattattgg ctattttgca
     3061 gggttactgc gtggtaccgt cggttttggt atcgacatca ttattgatgc catcaccggt
     3121 ttgtaccttt ttattgatat ccggacagcg accatctttg cacatggtgt tcttgtgctc
     3181 ttcatctttg gtcattccgt cattgttcat tgaagaacca tccgaatgca gcattgtgcc
     3241 accagaaccg gtatttaccc cgttattgtc gacgttattt ggcgcgacat tttcacgggc
     3301 gtcaggggct acctggcccg catcagctgc ggcgtttgcc tggccgttat tagtttgcgc
     3361 tccgctatcg gcagccagtg cggcaccgct ggcaaggctt agagtggcag tcagaaataa
     3421 tgtggccagt tttgtcattt tcataggatg ctcctgttat ggtcgttatg tcggataacc
     3481 tcttccaaca gtgcatttgc aggtgaatat aaggcattgg tttaagattt cagccaggtt
     3541 atgaaacgca gcagagaatc ttgaaataat taacaaacaa aggagttaca gttagaaatt
     3601 gtaggagaga tctcgttttt cgcgacaatc tggcgttttt cttgctaatt ccaggattaa
     3661 tccgttcata gtgtaaaacc ccgtttacac attctgacgg aagatataga ttggaagtat
     3721 tgcattcact aagataagta tggcaacact ggaacagaca tgaattatca gaacgacgat
     3781 ttacgtatca aagaaatcaa agagttactt cctcctgtcg cattgctgga aaaattcccc
     3841 gctactgaaa atgccgcgaa tacggttgcc catgcccgaa aagcgatcca taagatcctg
     3901 aaaggtaatg atgatcgcct gttggtggtg attggcccat gctcaattca tgatcctgtc
     3961 gcagcaaaag agtatgccac tcgcttgctg gcgctgcgtg aagagctgaa agatgagctg
     4021 gaaatcgtaa tgcgcgtcta ttttgaaaag ccgcgtacca cggtgggctg gaaagggctg
     4081 attaacgatc cgcatatgga taatagcttc cagatcaacg acggtctgcg tatagcccgt
     4141 aaattgctgc ttgatattaa cgacagcggt ctgccagcgg caggtgagtt tctcgatatg
     4201 atcaccccac aatatctcgc tgacctgatg agctggggcg caattggtgc acgtaccacc
     4261 gaatcgcagg tgcaccgcga actggcgtct ggtctttctt gtccggtagg ttttaaaaat
     4321 ggcacagacg gtacgattaa agtggctatc gatgccatta atgccgccgg tgcgccgcac
     4381 tgcttcctgt ccgtaactaa atgggggcat tcggcgattg tgaataccag cggtaacggt
     4441 gattgccata tcattctgcg cggcggtaaa gagcctaact acagcgcgaa gcacgttgct
     4501 gaagtgaaag aagggctgaa caaagcaggc ctgccagcac aggtgatgat cgatttcagc
     4561 catgctaact cgtccaaaca attcaaaaag cagatggatg tttgtgctga cgtttgccag
     4621 cagattgccg gtggcgaaaa ggccattatt ggcgtgatgg tggaaagcca tctggtggaa
     4681 ggcaatcaga gcctcgatag cggggagccg ctggcctacg gtaagagcat caccgatgcc
     4741 tgcattggct gggaagatac cgatgctctg ttacgtcaac tggcgaatgc agtaaaagcg
     4801 cgtcgcgggt aaggtttaat tgtcggatgc gccgtcagag tggcgtatcc gatgaatcac
     4861 cacaggcctg ataagtcgcg cagcgtcgca tcaggcaatg tgctccattg ttagcaacaa
     4921 aaaagccgac tcacttgcag tcggctttct cattttaaac gaatgacgtt tacttcgctt
     4981 taccctggtt tgcaaccgcc gctgctttcg ctgcgatctc gtcagcatta cccagataat
     5041 agcgtttcag cggtttgaaa ttctcgtcga actcatacac cagcggcacg ccagtcggga
     5101 tattaagctc aagaatctct tcttcgctca tgttatcaag atatttcacc agcgcacgta
     5161 aagagttacc gtgtgcagcg atgatcacgc gctcaccgct cttcatacgc ggcagaatag
     5221 tttcattcca gtaagggatc acgcggtcaa tggtcagcgc caggctttcc gtcagcggca
     5281 gttctttttc gctcagtttc gcgtaacgcg gatcgtgacc cggataacgc tcatcatctt
     5341 tagtcagttc cggcggagtc actgcaaaac cacgacgcca ctgtttcacc tgctcgtcgc
     5401 catacttttc agcagtttcc gctttgttca gaccctgcaa cgcaccgtag tgacgttcgt
     5461 tcagtttcca ggatttctca acgggcagcc atgcctgatc cagttcgtcc agcacattcc
     5521 acagggtatg gatagcgcgt ttcagcacag aggtgtaagc aaagtcaaag ctgtaacctt
     5581 cctctttcag cagcttacct gctgcttttg cttcgcttac gcctttctca gacagatcca
     5641 cgtcgtacca accggtgaaa cggttttctt tgttccactg actttcgcca tgacgaacca
     5701 gaaccagctt agttacagcc atatacttac tcctcaaatc attttttaat gataataatt
     5761 ctcattatat tgccgtgacg aagcaacagc aatgcttacg cataaccata gcgaaaatag
     5821 tgtcgcagtg taaggttgtt gtgaatattg agttgcaaat atgtcggtgt ttgctggtga
     5881 tttgagcaat ataaggataa agccctcatt acgagggctt caggtgctac tcagcaataa
     5941 actgatattc cgtcaggctg gaatactctt cgccaggacg caggaagcag tccggttgcg
     6001 gccattcagg gtggtttggg ctgtccggca gaaactcgct ttccagtgcc aggccttgcc
     6061 agtcggcgta aggttcagtt cctcttgatg gcgtgccgcc gaggaagttg ccggagtaga
     6121 attgcagagc cggagcggtg gtgtagacct tcagctgcaa tttttcatct gctgaccaga
     6181 catgcgccgc cactttcttg ccatcgcctt tggcctgtaa caagaatgcg tgatcgtaac
     6241 ctttcacttt gcgctgatcg tcgtcggcaa gaaactcact ggcgatgatt ttggcgctac
     6301 ggaaatcaaa agacgttccg gcgacagatt tcaggccgtc gtgcggaatg ccgccttcat
     6361 caaccggcag atattcgtcc gccagaatct gcaacttgtg attgcgcacg tcagactgct
     6421 cgccgtcaag attgaaatag acgtgattag tcatattcac cgggcaaggt ttatcaactg
     6481 tggcgcgata agtaatagag atacggttat cgtcggtcag acgatattgc accgtcgcgc
     6541 cgagattacc cgggaagccc tgatcgccat catctgaact cagggcaaac agcacctgac
     6601 gatcgttctg gttcacaatc tgccagcgac gtttgtcgaa cccttccggg ccgccgtgca
     6661 gctggttaac gccctgactt ggcgaaagcg tcacggtttc accgtcaaag gtataacggc
     6721 tattggcgat gcggttggca taacgaccaa tagaggcccc cagaaacgca gcctgatcct
     6781 gatagcattc cgggctggca cagccgagca gcgcctcgcg gacgctgcca tcggaaagcg
     6841 gaatacgagc tgaaagtaaa gtcgcacccc agtccatcag cgtgactacc atccctgcgt
     6901 tgttacgcaa agttaacagt cggtacggct gaccatcggg tgccagtgcg ggagtttcgt
     6961 tcagcactgt cctgctcctt gtgatggttt acaaacgtaa aaagtctctt taatacctgt
     7021 ttttgcttca tattgttcag cgacagcttg ctgtacggca ggcaccagct cttccgggat
     7081 cagcgcgacg atacagccgc caaatccgcc gccggtcatg cgtacgccac ctttgtcgcc
     7141 aatcacagct ttgacgattt ctaccagagt gtcaatttgc ggcacggtga tttcgaaatt
     7201 atcgcgcata gaggcatgag attccgccat caactcgccc atacgtttca ggtcgccttg
     7261 ctccagcgcg ctggcagctt caacggtgcg ggcgttttca gtcaggatat gacgcacgcg
     7321 tttcgccacg attgggtcca gctcatgtgc aacagcgttg aactcttcaa tggtgacatc
     7381 gcgcagagct ggctgctgga agaaacgcgc accggtttcg cactgttcac gacgggtgtt
     7441 gtattcgctg ccaaccaggg tacgtttgaa gttactgttg atgatgacga cagccacacc
     7501 tttcggcatg gaaactgctt tggtccccag tgaacggcag tcaatcagca aggcatgatc
     7561 tttcttgccg agcgcggaaa ttagctgatc catgatcccg cagttacagc caacaaactg
     7621 gttttctgct tcctgaccgt taagcgcgat ttgtgcgccg tccagcggca gatgataaag
     7681 ctgctgcaat acggttccga ccgccacttc gagtgaggcg gaagaactta acccggcacc
     7741 ctgcggcaca ttgccgctga tcaccatgtc cacgccgccg aagctgttgt tacgcagttg
     7801 cagatgtttc accacgccac gaacgtagtt agcccattga tagttttcat gtgcgacaat
     7861 gggcgcatcg agggaaaact cgtcgagctg attttcataa tcggctgcca tcacgcgaac
     7921 tttacggtca tcgcgtggtg cacaactgat cacggtttga taatcaatcg cgcagggcag
     7981 aacgaaaccg tcgttgtagt cggtgtgttc accaatcaaa ttcacgcggc caggcgcctg
     8041 aatggtgtga gtggcagggt agccaaatgc gttggcaaac agagattgtg ttttttcttt
     8101 cagactcatt tcttacactc cggattcgcg aaaatggata tcgctgactg cgcgcaaacg
     8161 ctctgctgcc tgttctgcgg tcaggtctcg ctgggtctct gccagcattt cataaccaac
     8221 cataaattta cgtacggtgg cggagcgcaa cagaggcgga taaaagtgcg cgtgcagctg
     8281 ccagtgttga ttctcttcgc cattaaatgg tgcgccgtgc cagcccatag agtaggggaa
     8341 ggagcactgg aagaggttgt cataacgact ggtcagcttt ttcaacgcca gcgccaaatc
     8401 gctgcgctgg cgtcggtcaa atcggtgatc cgcaaaacgt gggctttggg cagcagtagc
     8461 gtttcgaacg gccaggcagc ccagtaaggc acaacggcta accagtgttc ggtttcgaca
     8521 acggtacggc taccgtctgc cagctcgcgc tgaacataat ccaccagcat tggtgatttc
     8581 tgttcggcaa aatattcttt ttgcaggcgg tcttcgcgct cagcttcgtt aggcaggaag
     8641 ctatttgccc aaatctgtcc gtgcggatgc gggttagagc agcccatcgc cgcacctttg
     8701 ttttcaaaga cctgcaccca cgggtacgtt tttcccagct ctgcggtttg ctcctgccag
     8761 gttttgacga tttccgtcaa tgccgcaacg ctcagttctg gcaacgtttt actgtgatcc
     8821 ggtgaaaagc agatcacccg gctggtaccg cgcgcgctct ggcaacgcat caaaggatcg
     8881 ttgctttctg gcgcatctgg cgtgtcagac atcaaggccg caaagtcatt agtgaaaacg
     8941 taagtcccgg tgtaatcggg gtttttatcg cctgtcaccc gcacattacc tgcgcagagg
     9001 aagcaatctg gatcgtgcgc aggtaacacc tgtttggctg gcgtttcctg cgccccctgc
     9061 caggggcgct tagcgcggtg cggtgaaacc agaatccatt gcccggtgag cgggttgtag
     9121 cggcgatgtg gatgatcaac gggattaaat tgcgtcatgg tcgttcctta atcgggatat
     9181 ccctgtggat ggcgtgactg ccagtgccag gtgtcctgcg ccatttcatc gagtgtgcgc
     9241 gttacgcgcc agttcagttc acggtcggct ttgctggcgt ccgcccagta ggccggaagg
     9301 tcgccctcgc gacgcggtgc aaaatgataa ttaaccggtt tgccgcaggc tttgctgaag
     9361 gcattaacca cgtccagcac gctgttgcct acgccagcgc cgaggttgta gatgtgtacg
     9421 cctggcttgt tcgccagttt ttccatcgcc acgacgtgac cgtccgccag atccattacg
     9481 tggatgtaat cgcgtacgcc agtaccatct tcggtcggat aatcgttacc aaaaatcgcc
     9541 agcgagtcgc gacggcctac agcaacctgg gcgatgtatg gcatcaggtt attcggaatg
     9601 ccttgcggat cttcgcccat atcgcccgac ggatgcgcgc caaccgggtt gaagtagcgc
     9661 agcagggcaa tgctccagtc cggctgggct ttttgcagat cggtgaggat ctgttccacc
     9721 atcagcttgc ttttgccgta agggctttgt ggtgtgccgg tcgggaagct ttcaacgtat
     9781 ggaattttgg gctgatcgcc ataaacggtg gcggaggagc taaaaataaa gtttttgacg
     9841 ttagcggcgc gcatggcgct aatcaggcgc agagtgccgt tgacattgtt gtcgtaatat
     9901 tccagcggtt tttgtaccga ttcgccaacg gctttcagcc cggcgaagtg gatcacggtg
     9961 tcgatagcgt gatcgtgcag tatctcggtc atcaacgctt cgttacgaat atcgccttca
    10021 acaaacgttg gatgtttgcc gcctaaacgc tcgataacag gcagtacgct gcgcttactg
    10081 ttacagaggt tatcaagaat gatgacatca tgaccgtttt gcagtaattg cacacaggta
    10141 tgacttccaa tgtaaccgct accaccggta accagaactc tcataattcg ctccattagg
    10201 cttatggtat gaaataacca tagcataaca aagatgcgaa aagtgtgaca tggaataaat
    10261 tagtggaatc gtttacacaa gaatttagcc gttttttatg cgcgattaag tgattataaa
    10321 acagagggtt tatgaatgat tgcgcttttt atctgaaaaa agaagcggtt tcatgcctgc
    10381 atgcgtcgaa ccgttggccg gagagggcgc ttaggccgcc tccggcaagg tcagcactac
    10441 cgactcaata tatttttgtc agcacatagc gatagagtcc accgtccggc acgaactcaa
    10501 ggcgatgggt aatacaggca ggcgcatctt cagcgtggtg cgaaacaaac aacaattgcg
    10561 tttcaccttc gctaatcagc acatcaacaa aacggcggat aagctggcga ttgagcggat
    10621 caagcccctg tagtggttca tcgagaataa gcaacgtcgg atgtttcacc agtgcgcgga
    10681 cgatcagcgc cagacgctgc tgtccccagg aaagactatg gaacggagcg tcagccgtac
    10741 gtttatcaat gccgagaata tccagccact gctgcaccag tttttgctgg cgatccgaaa
    10801 cggcctgata aatgccaatc gaatcaaaat agccagaaag aatcacatta cgcacagtag
    10861 tactgacccg gtaatccaga tgcaaactac tgctgacgta accgatatgc tttttgatat
    10921 cccagatggt ttcgccgctg ccgcgacgtc gtccgaaaag cgtcaaatcg ttgctgtaac
    10981 cttgcggatg atcgccagta accaggctta ataacgtcga ttttcctgca ccatttggcc
    11041 cgacaatttg ccagtgttcg cctggattca cctgccagct aaggttatta agaatggggc
    11101 gatcgttata agaaaccacg ccattgttca gcacaatgcg cggttcgttg gcgggtaagg
    11161 cgtgacgtgc tgaaggttca tccggctccg gcagttgcac accttcaagc tgttcactat
    11221 gcgccagttg cgcgacgagt gcttgttgga gcagttcctc tttagcgcca gtttccgcta
    11281 acgtgcaatc cgccagcacg ccagcaaact ggacaaactc cgggatctca tcgaagcgat
    11341 tgagcaccag taccagagta ataccggact gatgtaacga ggcgagtcgc tcagccagct
    11401 gctgacgtga ggcaacatcc aggccatcga acggctcatc aagaatcaac aagtcaggct
    11461 ccgacatcag cgcctgacac agcagggttt ttcgcgtctc gccagtggaa aggtatttaa
    11521 agcgtcggtc gaggagggcg gtaataccga actgctgcgc cagttgcatg caacgcggtg
    11581 catcctttac ttcatcctga atgatctcag ccgtagtgcg tccggtgtca tcttcgccag
    11641 ggccgagcat atcggtgtta ttccgctgcc attcgtcgct gacgagtttt tgcaattgct
    11701 cgaaggagag acgagtgatg tgggaaaact ggctttgccg ttcacctttc aaaagcggaa
    11761 gttcccccgc cagcgcgcgg gccagcgccg atttcccgct tccattcgaa ccgacaaacg
    11821 cccaactatc acccgcgttt aacgttagct gaggcaattg cagcgttttt gtgtcgctaa
    11881 gacgaaacgc gccttgcaaa atttgcaacg atgacatttt atatcccact ttgtgcagcg
    11941 attactgaca gggatacgtg tttcataaca aattgtcaac acgcttagca cagcgtggcg
    12001 ataatcacgc tgtcggcatt aaagtaggcc gtgacattct gtccttgctg aagagaagtc
    12061 gcttcattta ccggcactgt ggcgcacagt gtttgcccgt ccggtagcga cattattact
    12121 tcgcactgct ctgcgccgcg ctcaatatgg ctaataatac ccggtaattg gttgtcagcg
    12181 ttttgcgcga ccgcttcgtc ctgagtaatg cctacccacg gcgcttttag caatatcaac
    12241 acttctttgc cttcatccag ccccagacgc gcgccgcttt gtgcggtaat tgcgactttc
    12301 aggcgtgttt ttccgtcagc cagtaagaca tcaacatgct gttgaacgtc atcatgatcg
    12361 cgggcggtga tggtaccgaa ccactggtta cgggcgctgg tttgcagtga aaaacgtgag
    12421 atcgcggcca acaggctgtt cagcggcagg gcgtcatcgt cacttaacac atcaaaggct
    12481 ttttgctgga tttgcgccag taagtcatag agctgaatca gtcgctgacc atagcgggtc
    12541 agtactgcgc cgccgccacc tttaccgcct gttgcgcgct cgaccagaat atgctcactt
    12601 aactgattca tctcgttaat ggcatcccag gcgcttttat agctgatacc ggcatctttc
    12661 gctccctggc taatggaacc ggaaagcgca atgtgtttta gtagcgaaat gcggcgcggg
    12721 tcggcgaata atttttgttg gagcttaagg gtgagaagga tttcggcctg cataacaatg
    12781 tcctggcaaa agtcttattg tgacggaaaa cgaacgccac gcaaagctga ccgcacaaaa
    12841 ggggagtgct tttctgtgct tagcggttag aatagtctca tgactatatc tggagttgac
    12901 catgttagag ttattaaaaa gtctggtatt cgccgtaatt atggtacctg tcgtgatggc
    12961 catcatcctg ggtctgattt acggtcttgg tgaagtattc aacatctttt ctggtgttgg
    13021 taaaaaagac cagcccggac aaaatcattg attccctgaa tgcccgctta gtcgggcatt
    13081 ttctttttct caacttcctg cttttcctgc cgatattttt tcttatctac ctcacaaagg
    13141 ttagcaataa ctgctgggaa aattccgagt tagtcgttat attgtcgcct acataacgtt
    13201 acattaaggg gttaccaatg gctcgtaaat ggttgaactt gtttgccggg gcggcactct
    13261 ctttcgctgt tgctggcaat gcactggcag atgaagggaa aatcacggtg ttcgccgccg
    13321 catcactgac taacgcaatg caggacattg ctacgcagta taaaaaagag aaaggcgtgg
    13381 atgtggtttc ttctttcgct tcgtcatcta ctctcgcccg tcagattgaa gcgggtgccc
    13441 ctgcggatct gtttatttct gccgatcaga aatggatgga ttatgcggtt gataaaaaag
    13501 cgatcgatac tgctacgcgt cagacactgc tcggcaatag cctggtcgtt gtagcaccga
    13561 aagccagcga gcagaaagat ttcaccatcg acagcaaaac taactggact tcgctgctga
    13621 atggcggtcg cctcgcggtt ggcgatccgg aacatgtccc tgctggcatt tatgcaaaag
    13681 aagcactgca aaaactgggc gcatgggata cgctctcccc gaaactggct ccggcggaag
    13741 atgttcgtgg cgcgctggcg ttggtcgaac gcaatgaagc gccgttgggc attgtttacg
    13801 gttctgacgc agttgccagc aaaggggtaa aagtggttgc catcttcccg gaagattcac
    13861 ataaaaaagt ggaatatccg gttgctgttg tggaagggca taacaatgcg acagtgaaag
    13921 cattttatga ttatctgaag ggaccgcagg ctgcggaaat ctttaaacgt tacggattta
    13981 caaccaagta atgatactga ccgatccaga atggcaggca gttttattaa gcctgaaagt
    14041 ttcttccctg gctgtgctgt ttagcctgcc gtttgggatc ttttttgcct ggttactggt
    14101 gcgttgcacg tttccgggca aagctctgct cgacagcgta ctgcatctac cgctggtgtt
    14161 accgcccgtg gtcgtcggtt acttattatt agtttcgatg ggacggcgcg gatttatcgg
    14221 tgaacgtctg tatgactggt ttggtattac cttcgccttt agctggcgcg gcgcggttct
    14281 cgctgccgcc gtcatgtcgt ttccgctgat ggtgcgggca attcgtctgg cgctggaagg
    14341 ggttgatgtc aaactggaac aggccgcaag aacactgggg gccgggcgct ggcgcgtttt
    14401 ctttactatc acgttaccgc tgaccttacc gggaattatt gttggtacgg tactggcttt
    14461 tgctcgttct ctcggtgagt ttggtgcaac catcaccttt gtgtcgaaca ttcctggtga
    14521 aacgcgaacc attccttctg ccatgtatac cctgatccag acccccggcg gggaaagtgg
    14581 agcggcgaga ctgtgcatta tttctattgc gctggcgatg atctccctgt tgatttcaga
    14641 atggctggcc agaatcagcc gtgaacgggc ggggcgctaa tcatgctgga actgaatttt
    14701 tcccagacgt tgggcaacca ttgcctgact attaatgaaa cgctgcccgc caatggcatc
    14761 actgctatct ttggcgtctc cggtgccgga aaaacttcgc tgattaacgc catcagtgga
    14821 ctgacgcgcc cgcaaaaagg gcggattgtc ctcaatgggc gggtactaaa tgatgccgaa
    14881 aaaggtatct gcctgacgcc ggaaaagcgt cgcgttggct atgtttttca ggatgcgcgg
    14941 ctgttcccgc attacaaagt gcgtggcaat ctgcgctacg gcatgtcgaa aagtatggtc
    15001 gatcagttcg ataagctggt ggcgctttta ggcattgaac cgttgcttga ccgtttacca
    15061 ggcagcctgt ccggaggcga aaaacagcgc gtggcgattg gtcgggcttt gctgacagca
    15121 ccggaattgc tgttgctgga tgaaccgctg gcgtcactgg atattccgcg taaacgcgaa
    15181 ctgttgcctt atctgcaacg gctgacacgg gaaatcaaca ttccgatgtt gtatgtcagc
    15241 cattcgctgg atgagatcct ccatctggca gacagagtga tggtactgga aaacggtcag
    15301 gtgaaagcct ttggcgcgct ggaggaagtg tggggcagta gcgtgatgaa tccgtggctg
    15361 ccgaaagagc aacaaagtag cattctgaaa gtgacggtgc ttgagcatca tccgcattac
    15421 gcgatgaccg cgctggcgct gggcgatcag catttgtggg tcaataagct ggacgaaccg
    15481 ctgcaagctg cgctacgtat ccgcattcag gcttccgatg tttctctggt tttacaaccg
    15541 ccgcagcaaa ccagcattcg taacgtattg cgggcaaaag ttgttaatag ttatgacgac
    15601 aacggccagg tggaagtgga actggaagtc ggcggtaaaa cgctgtgggc gcgtatcagc
    15661 ccgtgggcca gggatgaact ggcgatcaaa cctggcctgt ggctgtacgc gcaaattaaa
    15721 agtgtgtcga taaccgcctg attaaatcag gtggctataa atgaactggg caatgctgtc
    15781 ggtggtgtta tcaccaatca caatgttggc gcgcgctttt accgcgtcat cggcgttgcc
    15841 catcgccacg cctgtaccag cggcttccag catactgata tcattaaagt tatcgccgaa
    15901 tgccacgacg ttttccatcg accaaccttg cgcctcaacc catttcgtca aacgtttacc
    15961 tttgctgttg ccgccgcgtg caatatcaac ctgatcgtgc caggaccatt cacactccag
    16021 tcccagttca tgttcgacat gcttaccaaa atgctgcaat tgcggcaggt catcgtgcgt
    16081 cagggcgaac ttccatacgg cgttaacttg ttgcgccgtt tcagccagag aagcgacttg
    16141 tgtgaaagtc ggacgctgtt ccggcggcag ggtttgcgcc cagttagacg tgcgaatgac
    16201 atgcccggtc gggtgctcat agaccattgc atcatccaca tacatcagac cgtgaatgtg
    16261 gtgttcattc agcatctcaa tgagttgcag ggctttatta acgggcattg ggtccgcttc
    16321 cagcacggtt tttgcatgat aatcatacaa ataggtgcca ttacagcaaa tagcaggtgt
    16381 atccagcgcc agcgcctgat aaaaaggatg aatagcgacg tgatggcgac ctgtgacgat
    16441 gattaattga tagcctgctt cgcgagcgcg ggccagggct tctatcgatg aaggaagcag
    16501 ggtctttttc ggggtcaata aggtgccgtc taagtcgaga gcaatcacgc gtgtggtcat
    16561 ggcgtattcc agattaaggt taagaatttt cgtctgcgcg aatggtacac cgataccact
    16621 atcggacaaa attctgcgtt ttaattcagc attcaccgcc aaaagcgact aattttagct
    16681 gttacagtca gttgctaaat gcaaaggagc attcatgaag caaacagttt atatcgccag
    16741 ccctgagagc cagcaaattc acgtctggaa cctgaatcat gaaggcgcac tgacgctgac
    16801 acaggttgtc gatgtgccgg ggcaggtgca gccgatggtg gtcagcccgg acaaacgtta
    16861 tctctatgtt ggtgttcgcc ctgagtttcg cgtcctggcg tatagtatcg ccccggacga
    16921 tggcgcactg acctttgccg cagagtctgc gctgccgggt agtccgacgc atatttccac
    16981 cgatcaccag gggcagtttg tctttgtagg ttcttacaat gcgggtaacg tgagcgtaac
    17041 gcgtctggaa gatggcctgc cagtgggcgt cgtcgatgtg gtcgaggggc tggacggttg
    17101 ccattccgcc aatatctcac cggacaaccg tacgctgtgg gttccggcat taaagcagga
    17161 tcgcatttgc ctgtttacgg tcagcgatga tggtcatctc gtggcgcagg accctgcgga
    17221 agtgaccacc gttgaagggg ccggcccgcg tcatatggta ttccatccaa acgaacaata
    17281 tgcgtattgc gtcaatgagt taaacagctc agtggatgtc tgggaactga aagatccgca
    17341 cggtaatatc gaatgtgtcc agacgctgga tatgatgccg gaaaacttct ccgacacccg
    17401 ttgggcggct gatattcata tcaccccgga tggtcgccat ttatacgcct gcgaccgtac
    17461 cgccagcctg attaccgttt tcagcgtttc ggaagatggc agcgtgttga gtaaagaagg
    17521 cttccagcca acggaaaccc agccgcgcgg cttcaatgtt gatcacagcg gcaagtatct
    17581 gattgccgcc gggcaaaaat ctcaccacat ctcggtatac gaaattgttg gcgagcaggg
    17641 actactgcat gaaaaaggcc gttatgcggt cgggcaggga ccaatgtggg tggtggttaa
    17701 cgcacactaa ccgctgattt aaccggcgca gtctctcctg cgccggtgta ttaacctatc
    17761 tcctgtaacg catgtctctg gcgttcgacg atattggtcc acaaattgtc tttatcgtca
    17821 gtccacaaat taatcagcaa ggcaaaaaag cgttctgctg ccggagaaag cacggcatct
    17881 ttgcggcgaa taatccccaa tgtccgacgt ataacgggtt ccaccagtgg aataccaata
    17941 atcgaagaat agggcgcatg gggcattgcc agaccaggaa gcgccgaaat ccccagtccc
    18001 gcctctacca gtcctaatga cgtcgaaaga tgacgcactt cgtaaaacca gtccagcttc
    18061 cagggcttgt cggctagctg ttgttctatc aacaatcggt tgccgctgga ggaacgtacg
    18121 ccaatcattt tgtaacccac taactcctgc cattctacaa gctgcttttt ggccaatgga
    18181 tggtcacgcc gacaggccag cacgaatggc tcgttgacca gtggggtaaa atcaatggat
    18241 gaatttgtga cgttgttcat gtttatgcca aagtctgatt cgttacacag caccgattcc
    18301 atgcaattat tcgtgccttg ttccagaatc cgcactttaa tattgggata cagctcatta
    18361 aatttaccga ttgccagcgg taaaaaataa aatactgcgg tcggaataca cgctaacgtc
    18421 accataccac gatgataagc attcatatca cgaatattaa aaagcgtttc atcgaatttc
    18481 tttattaatt cccgcgcctc cgggagcaac ctttttcctg cttttgttaa cgtaaccttg
    18541 cgagttgtac gctcaaatag ttgaatatgt aaatcttcct ccatcttttt tattcttcgc
    18601 gttaatgcag gttgcgtaat attgagtaat ttagcagcat tgttaaatga actggactct
    18661 gccagtatga caaatgcctt catacttgat aattcatgct tcattattac tccggaaaat
    18721 ggaagcgacg attttgggtg gctggtcgtt aaaaatttta actgcattta gccaacttaa
    18781 attaatgaaa aaatgttatt aatcgttgag ctaaagtcat tagagatgct ttgtccttaa
    18841 tgtaaccata tcgcaataag ttatgttttt aaattgaggg cattattatg aaaaaaatac
    18901 cctgcgtgat gatgcgaggt ggaacctcga ggggcgcgtt cctgttagcg gaacatttac
    18961 ccgaagatca aacgcagcgc gataaaatat tgatggcaat tatgggttcc ggtaacgatc
    19021 tggaaattga cggtattggc ggcggtaatc cactgaccag taaagtcgcc attattagcc
    19081 gttccagcga tccgcgtgct gatgttgatt atctgtttgc tcaagttatc gtccatgagc
    19141 aacgtgtcga taccacgcct aactgcggca atatgctttc tggcgttggg gcattcgcca
    19201 ttgaaaatgg tttgattgca gcgacatcac cggtcacccg tgtgcgtatc cgcaacgtca
    19261 atacgggtac gttcatcgaa gctgatgtgc aaacgccaaa tggtgttgtc gagtacgagg
    19321 gtagtgccag aattgacggc gtaccgggta ctgccgcacc ggttgcgctc actttcctga
    19381 atgccgctgg aaccaaaacc ggaaaagttt tcccgactga taatcagatt gattattttg
    19441 acgatgtccc ggtgacctgt atcgatatgg cgatgccagt cgtcattatt ccggctgaat
    19501 atctgggaaa aacaggttat gaattaccgg cggaactgga tgccgacaaa gcattattag
    19561 cccgcattga atctatccgt ctacaagcgg gtaaagcaat gggcttaggt gatgtcagta
    19621 atatggttat ccctaaacct gtgcttattt ctccagcgca gaaaggcggg gcaattaatg
    19681 tgcgttattt tatgccgcat tcttgccatc gcgcgctggc gataaccggt gctattgcta
    19741 tttccagtag ttgtgcattg gaaggcaccg tcacccgaca aatcgtccct tctgtaggat
    19801 acggcaatat caatattgaa caccccagtg gtgcgctcga cgttcattta agtaatgaag
    19861 gtcaggatgc cacgacgtta cgcgcatctg ttattcggac gaccagaaaa atattttccg
    19921 gtgaagttta tcttccctga aaaaattcgt tgtcaagata aggataatca ataaaggact
    19981 tctgtatgag tcatacagaa agaacaggat tttaaatgaa taagaaatcg ttatggaagc
    20041 taattctgat attagcgatc ccatgtatta ttggttttat gccagctccg gcaggattaa
    20101 gcgaactggc gtgggtgctt tttggtattt acctggcggc cattgtgggg ctggttatca
    20161 agcctttccc ggaacctgtc gtactgttaa ttgccgttgc tgcctccatg gtggtggtcg
    20221 gtaacttatc cgacggtgcg tttaagacca ccgccgtatt aagcggttac tcttcaggta
    20281 ctacctggct ggtgttctcg gcgtttacct taagcgccgc atttgtgacc accggtttag
    20341 gtaaacgtat tgcctatctg ctgattggta aaatcggtaa caccacgctg ggtctgggtt
    20401 acgttacggt attcctcgat ctggtactgg ctccggcaac accgtctaac accgcgcgtg
    20461 cgggcggtat tgtgttaccg atcatcaaca gcgtggcggt ggctttgggg tccgaaccgg
    20521 aaaaaagtcc gcgtcgtgtc ggacattacc tgatgatgtc catttacatg gtcaccaaaa
    20581 ccaccagcta tatgttcttt accgcaatgg cggggaacat tctggcgctg aaaatgatca
    20641 acgacattct gcacctgcaa attagctggg gtggatgggc actggccgcc ggattgccgg
    20701 gcatcattat gctgctggtc accccactgg tgatttatac catgtatccg ccagaaatta
    20761 agaaggtgga taataaaacc atcgctaaag cgggccttgc cgaactggga ccgatgaaaa
    20821 tccgcgaaaa aatgctgctc ggtgtctttg tgctggcgct actgggctgg attttcagta
    20881 agtctctggg ggttgatgaa tccactgtgg caatcgttgt tatggcgacc atgctgctgc
    20941 tgggtatcgt tacctgggaa gacgtggtta aaaataaagg cggctggaat accttaatct
    21001 ggtacggcgg tattatcgga ttaagctcct tattatcgaa agttaaattc ttcgaatggt
    21061 tagctgaagt ctttaaaaat aacctggcat ttgatggtca cggtaacgtt gctttcttcg
    21121 ttattatttt cctcagcatt atcgtgcgtt atttcttcgc ttccggtagt gcctatatcg
    21181 ttgctatgtt accggtattt gccatgctgg cgaacgtctc cggcgcaccg ttaatgttaa
    21241 ccgcgctggc actgttgttc tccaactcct atggcggcat ggttactcac tatggcggcg
    21301 cggcaggtcc ggtcatcttt ggcgtgggtt ataacgatat taaatcctgg tggttggtcg
    21361 gtgcggtact gacgatatta accttcctgg tgcatatcac cctcggcgtg tggtggtgga
    21421 atatgctgat cggctggaac atgctgtaaa tattctcgtc atacttcaag ttgcatgtgc
    21481 tgcgtctgcg ttcgctcacc ccagtcactt acttatgtaa gctcctgggg attcactcgc
    21541 ttgtcgcctt cctgcaactc gaattattta gagtatatcc atttattatc tttctgcgca
    21601 cttcacggtg cgcagatatc tggagcattt gatgatcaag ttatctgaaa aaggcgtgtt
    21661 tctcgccagt aataacgaaa taattgccga agaacatttc accggcgaaa ttaaaaaaga
    21721 agaagcccaa aaaggcacta ttgcctggtc tattctctct tcgcataata cgtccggaaa
    21781 tatggataaa cttaaaatta agtttgattc attagcctct cacgatatta cctttgttgg
    21841 tattgtacag accgctaaag cgtccggtat ggaacgtttc ccgctgccgt atgtgctgac
    21901 caactgccat aactcactct gcgccgtcgg cggcactatt aacggtgatg accatgtttt
    21961 tggtttatcg gcggcccagc gttatggcgg tatttttgtg cctccgcata ttgcggtcat
    22021 ccatcaatat atgcgtgaga tgatggcagg cggcggcaaa atgatcctcg ggtcagacag
    22081 ccacacccgt tacggtgcat tagggacaat ggcagtcggt gagggcggcg gtgagttggt
    22141 aaaacagctg cttaatgaca cctgggatat cgactatccg ggcgtggttg cggtgcatct
    22201 gaccggaaaa cctgcgccgt atgtggggcc acaggatgtg gcgctggcta tcattggcgc
    22261 ggtgttcaaa aacggttacg tcaaaaacaa agtcatggag ttcgttggac cgggcgttag
    22321 cgcgctctct accgatttcc gtaacagcgt tgacgtgatg accacggaaa ccacctgttt
    22381 aagctccgtc tggcaaactg atgaagaagt gcataactgg ctggcgctgc acggtcgcgg
    22441 ccaggattac tgccagctta accctcaacc gatggcgtac tacgatggct gcatcagcgt
    22501 tgatttaagc gccatcaaac caatgattgc gctgccgttc cacccgagca acgtgtataa
    22561 aatcgacaca ctgaaccaga acctgaccga cattctgcgt gagattgaaa ttgagtccga
    22621 acgcgtggcg cacggtaaag ccaaactctc gctgctggat aaagtggaaa atggtcgcct
    22681 gaaagtgcag caggggatta tcgcgggctg ttctggcggt aactacgaaa acgtcatcgc
    22741 ggcggcgaat gcactgcgcg gtcaatcctg tggcaatgac accttctcgc tggcagttta
    22801 cccgtcatca cagccggtgt ttatggatct cgccaaaaaa ggtgtggtag cagatttgat
    22861 tggcgcaggc gcaatcatca gaaccgcgtt ctgcggccca tgctttggcg cgggcgatac
    22921 gccaatcaac aacggtttga gtattcgcca caccacgcgt aacttcccga accgcgaagg
    22981 ctctaagcca gctaatgggc agatgtcagc ggtggcgttg atggacgctc gttctatcgc
    23041 tgcgactgcg gcaaacggtg gctatttaac ctctgccagc gaacttgatt gctgggacaa
    23101 cgtgccggag tacgccttcg atgtaacgcc gtataaaaac cgtgtttatc agggctttgt
    23161 gaaaggggca actcagcaac cgctgattta cggaccgaac attaaagact ggccggaatt
    23221 gggtgcgctg actgacaata tcgtcctgaa agtgtgctcg aagatcctcg acgaagtgac
    23281 caccaccgac gaactgattc cttccggtga aacctcttct tatcgttcaa atccgattgg
    23341 tctggcggag tttaccctgt cacgccgcga tcccggttat gttggcagaa gtaaagcgac
    23401 tgctgagctg gaaaatcagc gtctggcggg gaatgtcagc gagctgacag aggtgtttgc
    23461 gcgcattaag cagattgctg gtcaggagca tattgatccg ctgcaaactg aaattggcag
    23521 catggtatat gcggtgaaac caggcgatgg ttctgcgcgt gaacaggcgg cgagctgcca
    23581 gcgtgtgatt ggcggtctgg cgaatattgc cgaagagtac gcgactaaac gctaccgttc
    23641 taacgtcatc aactggggga tgttaccgct gcagatggcg gaagtgccaa cctttgaagt
    23701 gggggattac atttacatcc ctggcattaa agcggcgctg gataatccgg gtacgacgtt
    23761 taaaggttat gtgatccatg aagatgcgcc ggtaacggaa attacgctct atatggaaag
    23821 tctcactgct gaagagcgcg agattatcaa ggcgggtagt ttgattaact tcaataaaaa
    23881 ccgtcagatg taaaaagcgc catgtgaatg taggtcgcat tcggcactta ttgtcggatg
    23941 cgatgcttgc gcatcttatc cgacctacga atcgcatcga atctgtaggc cagataaggc
    24001 attttcgcag catccggcac ttattgtcgg atgcgatgct tgcgcatctt atccgaccta
    24061 caaatcgcat cgaaccgtag gccggataag gcgtttacgc cgcatccggc aaatagttaa
    24121 ttgctcttac ttcttcgcct ctgcaaccac tttactaccc acgccgcggt tattgtattc
    24181 ccacatgcgg ttgtagttag tgtcattcag attgcgctgt atttcgtcgt tatcatctac
    24241 gctgccggta ttacccgcaa acggacgatt agagatcacc gcatcggccc acggtttagc
    24301 cgtgttaaaa ccttcgttga tggcgctatc acggatcacc acctgaccgt tggtattggc
    24361 atcaacatcc agcgagcggc ccagttgcgc cacaccatca ccgaaagcat tgaaacggct
    24421 gtttacggcg aggaaaccgt agtaaatgtt ggacagcgta gccggtgcaa acacatacgc
    24481 ttcttgctga gtacgtgagt tcaccacgcg gaattcggtg ttatcgaaca ccactgcgcc
    24541 gcgaccagaa acgatatcca catccccttc aatgtagctg ttggtcacca gcgtacgcgg
    24601 ctgacgattc gtttccagac ggttctgcac accgctgttg gtgacaaaga aggtgttctg
    24661 acgaccgaga atgttaacgt tgttaatctg tacctggtca ccatcagtac gcagtgccac
    24721 cgccggatgg ttacctgcat ctacgctatc gcccagcgtg ttttcgatgg tcagattttg
    24781 cagttgcagg ccattgtttt gtgaccagaa gaccgcagag cagagaacac cgatactgtc
    24841 gctgcgtttg ctctggcagc tatcgtacat ataccacgct ggtttacctg gcatatattt
    24901 gccgcgcggg ttgacgtcgt gacgccagtc ggcagggctc atgccaccat caagggaaag
    24961 cccaatcttc acatcaatcg gtttttcacc tgtaccgtac agagtaattc cacccggagc
    25021 ggcagggaca tataccgttc cctgatactc accaggcatc acggcaatat actggcgctt
    25081 gttggtacgc ttgatgattg ccgcatctac cgccgcctga atcgtggtat gcgttacacc
    25141 ttgagtgccc gccgggccga caacaaagtc aggttgcgca ggcagggtaa tcggggaagg
    25201 attccacgct gctgcacctg gtgtcaggga tgcaaaatag tgttgagcat cgaaattctg
    25261 cgcttctttt gccgacagaa tcgggcgaga agaggtacca ggcgcggttt gatcagaagg
    25321 acgttgatcg ggcggggttg agctacaggc ggtcagcgtc acgccaaaag ccaatgccag
    25381 cgccagacgg gaaactgaaa atgtgttcac aggttgctcc gggctatgaa atagaaaaat
    25441 gaatccgttg aagcctgctt ttttatacta agttggcatt ataaaaaagc attgcttatc
    25501 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gatttcaatt
    25561 ttgtcccact ccctgcctct gtcatcacga tactgtgatg ccatggtgtc cgacttatgc
    25621 ccgagaagat gttgagcaaa cttatcgctt atctgcttct catagagtct tgcagacaaa
    25681 ctgcgcaact cgtgaaaggt aggcggatcc ccttcgaagg aaagacctga tgcttttcgt
    25741 gcgcgcataa aataccttga tactgtgccg gatgaaagcg gttcgcgacg agtagatgca
    25801 attatggttt ctccgccaag aatctctttg catttatcaa gtgtttcctt cattgatatt
    25861 ccgagagcat caacatgcaa tgctgttggg atggcaattt ttacgcctgt tttgctttgc
    25921 tcgacataaa gatatccatc tacgatatca gaccacttca tttcgcataa atcaccaact
    25981 cgctgcccgg taacaacagc cagttccatt gcaagtctaa gccaacatgg tgatgattct
    26041 gctgcttgat aaattttcag gtattcgtca gccgtaagtc ttgatctcct tacctctgat
    26101 tttgcagcgc gagtggctgc gaccgggttt gttgttatat ggccttcagc tatagcctct
    26161 cggaatgcat cgctcagtgt tgatctgatt aacttggctg atgccgcctt gccctcgtct
    26221 atgtatccat tgagcattgc cgcaatttct tttgtggtga tgtcttcaag tggagcatca
    26281 ggcagacccc tccttattgc tttaattttg ctcatgtaat ttatgagtgt cttctgcttg
    26341 attcctctgc tggccaggat tttttcgtag cgatcaagcc atgaatgtaa cgtaacggaa
    26401 ttatcactgt tgattctcgc tgtcagaggc ttgtgtttgt gtcctgaaaa taactcaatg
    26461 ttggcctgta tagcttcagt gattgcgatt cgcctgtctc ggcctaatcc aaactcttta
    26521 cccgtccttg ggtccctgta gcagtaatat ccattgtttc ttatataaag gttagggggt
    26581 aaatcccggc gctcatgact tcgccttctt cccatttctg atcctcttca aaaggctacc
    26641 tgttactggt cgatttaagt caacctttac cgctgattcg tggaacagat actctcttcc
    26701 atccttaacc ggaggaggga atatcctgca ctcgcgtacc catcgacgaa ctgtttcaag
    26761 gcttcttggg cgtcgctggc gtgcgttcca ctcctgaagt gtcaagtaca tcgcaaagtc
    26821 tccgcaatta cacgcaagaa aaaaccgcca tcaggcggct tggtgttctt tcagttcttc
    26881 aattcgaata ttggttacgt ctgcatgtgc tatctgcgcc cacagcatcc agtggtcata
    26941 gcagtcgctg atgttctcgg cttcgataac tctgttgaat ggttctccat tccattcacc
    27001 tgtaactcgg aagtgcattt atcatcgcca taaaacaaaa ctcgccgtag cgagttcaga
    27061 taaaagaaat ccccgcgagt gcgaggatag ttacttgttc atattattaa tcgtcaatgt
    27121 attttgagca ttgtgggcaa tcatcgatcc cacaatacga ttcatatgca tcctttattg
    27181 cgtcgcgggc ttcagt
//
LOCUS       DAMZET010000045        26931 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.045, whole genome shotgun sequence.
ACCESSION   DAMZET010000045 DAMZET010000000
VERSION     DAMZET010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.045"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(176..252)
                     /locus_tag="POB34_002014"
     tRNA            complement(176..252)
                     /locus_tag="POB34_002014"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(216..218),aa:Arg,seq:acg)
     gene            complement(256..348)
                     /locus_tag="POB34_002015"
     tRNA            complement(256..348)
                     /locus_tag="POB34_002015"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(312..314),aa:Ser,seq:gct)
     gene            complement(664..849)
                     /gene="csrA"
                     /locus_tag="POB34_002016"
     CDS             complement(664..849)
                     /gene="csrA"
                     /locus_tag="POB34_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDI5918964.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1084..3714)
                     /gene="alaS"
                     /locus_tag="POB34_002017"
     CDS             complement(1084..3714)
                     /gene="alaS"
                     /locus_tag="POB34_002017"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDI5918965.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3842..4342)
                     /gene="recX"
                     /locus_tag="POB34_002018"
     CDS             complement(3842..4342)
                     /gene="recX"
                     /locus_tag="POB34_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDI5918966.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLVAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4585..5646)
                     /gene="recA"
                     /locus_tag="POB34_002019"
     CDS             complement(4585..5646)
                     /gene="recA"
                     /locus_tag="POB34_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDI5918967.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5726..6223)
                     /gene="pncC"
                     /locus_tag="POB34_002020"
     CDS             complement(5726..6223)
                     /gene="pncC"
                     /locus_tag="POB34_002020"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HDI5918968.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6368..7453)
                     /gene="mltB"
                     /locus_tag="POB34_002021"
     CDS             complement(6368..7453)
                     /gene="mltB"
                     /locus_tag="POB34_002021"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HDI5918969.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7709..8272
                     /gene="srlA"
                     /locus_tag="POB34_002022"
                     /gene_synonym="gutA"
     CDS             7709..8272
                     /gene="srlA"
                     /locus_tag="POB34_002022"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDI5918970.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAVFEKKMGIQLEQKVHLAGATS"
     gene            8269..9228
                     /gene="srlE"
                     /locus_tag="POB34_002023"
                     /gene_synonym="gutE"
     CDS             8269..9228
                     /gene="srlE"
                     /locus_tag="POB34_002023"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDI5918971.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9239..9610
                     /gene="srlB"
                     /locus_tag="POB34_002024"
                     /gene_synonym="gutB"
     CDS             9239..9610
                     /gene="srlB"
                     /locus_tag="POB34_002024"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDI5918972.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9614..10393
                     /gene="srlD"
                     /locus_tag="POB34_002025"
     CDS             9614..10393
                     /gene="srlD"
                     /locus_tag="POB34_002025"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDI5918973.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10498..10857
                     /gene="gutM"
                     /locus_tag="POB34_002026"
     CDS             10498..10857
                     /gene="gutM"
                     /locus_tag="POB34_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDI5918974.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10924..11697
                     /gene="srlR"
                     /locus_tag="POB34_002027"
                     /gene_synonym="gutR"
     CDS             10924..11697
                     /gene="srlR"
                     /locus_tag="POB34_002027"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HDI5918975.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11690..12655
                     /gene="gutQ"
                     /locus_tag="POB34_002028"
     CDS             11690..12655
                     /gene="gutQ"
                     /locus_tag="POB34_002028"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HDI5918976.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12652..14166)
                     /gene="norR"
                     /locus_tag="POB34_002029"
     CDS             complement(12652..14166)
                     /gene="norR"
                     /locus_tag="POB34_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HDI5918977.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLSRATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14353..15792
                     /gene="norV"
                     /locus_tag="POB34_002030"
     CDS             14353..15792
                     /gene="norV"
                     /locus_tag="POB34_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HDI5918978.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALKLCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            15789..16922
                     /gene="norW"
                     /locus_tag="POB34_002031"
     CDS             15789..16922
                     /gene="norW"
                     /locus_tag="POB34_002031"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HDI5918979.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGIQA
                     TLDRQRNIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNTDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(17050..19302)
                     /gene="hypF"
                     /locus_tag="POB34_002032"
     CDS             complement(17050..19302)
                     /gene="hypF"
                     /locus_tag="POB34_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HDI5918980.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAEFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAHEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALAGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRFFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19455..19982)
                     /gene="hydN"
                     /locus_tag="POB34_002033"
     CDS             complement(19455..19982)
                     /gene="hydN"
                     /locus_tag="POB34_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HDI5918981.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(20131..21141)
                     /gene="ascG"
                     /locus_tag="POB34_002034"
     CDS             complement(20131..21141)
                     /gene="ascG"
                     /locus_tag="POB34_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HDI5918982.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVEMLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            21401..22858
                     /gene="ascF"
                     /locus_tag="POB34_002035"
     CDS             21401..22858
                     /gene="ascF"
                     /locus_tag="POB34_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HDI5918983.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKYQSVQPTVAKEVSLN"
     gene            22867..24291
                     /gene="ascB"
                     /locus_tag="POB34_002036"
     CDS             22867..24291
                     /gene="ascB"
                     /locus_tag="POB34_002036"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HDI5918984.1"
                     /translation="MSVFPESFLWGGALAANQSEGAFREGDKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDEFAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24405..24824)
                     /locus_tag="POB34_002037"
     CDS             complement(24405..24824)
                     /locus_tag="POB34_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5918985.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(24821..25132)
                     /locus_tag="POB34_002038"
     CDS             complement(24821..25132)
                     /locus_tag="POB34_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HDI5918986.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(25306..26067)
                     /locus_tag="POB34_002039"
     CDS             complement(25306..26067)
                     /locus_tag="POB34_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918987.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLEG"
     gene            complement(26092..26562)
                     /gene="hycI"
                     /locus_tag="POB34_002040"
     CDS             complement(26092..26562)
                     /gene="hycI"
                     /locus_tag="POB34_002040"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HDI5918988.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(26555..>26931)
                     /gene="hycH"
                     /locus_tag="POB34_002041"
     CDS             complement(26555..>26931)
                     /gene="hycH"
                     /locus_tag="POB34_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HDI5918989.1"
                     /translation="KFIDENDATPAEAQQVVYYSLAIGHHLGVIDCLEAALTCPWDEY
                     LAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAAQTSQQQEWSTML
                     LSMLHDIHQENAIYLMVRRLRD"
BASE COUNT         6347 a         7365 c         6838 g         6381 t
ORIGIN      
        1 agattttttg ataccgctac taaagccgta tcaagtaaga gatggtgcat ccgggaggat
       61 tactcgcctt gcggctcgcc cttcgggccg ttgctaaagc aacgttatcc tccctggtgc
      121 ctgcggttat accgccacct cttccatacc ttcatcgctg aaagtacgta gaatatggtg
      181 catccgggag gattcgaacc tccgaccgct cggttcgtag ccgagtactc tatccagctg
      241 agctacggat gcaaatggcg gtgaggcggg gattcgaacc ccggatgcag cttttgaccg
      301 catactccct tagcagggga gcgccttcag cctctcggcc acctcaccac acgcctctta
      361 cgagtgcttc gaagaacttg tttatcgctc atcgtcgctg cgtggcgcac atattacttt
      421 ctgagactta taagtcaaac aatttttcct acacttttat cgtttgcaca cttcacgttc
      481 aattagtctg taaaaacgtc aaaaagagtg ttttatcaac agaaaaatgg aggtctgaca
      541 gatagtagta atgcaaaaaa atggagactt aagttgaatg aacgggagta aagcgaaaag
      601 actatagagt gaaggagaaa ttttgagggt gcgtctcacc gataaagatg agacgcggaa
      661 agattagtaa ctggactgct gggatttttc agcctggata cgctggtaga tctcttcacg
      721 gtgaacagaa acttccttcg gggcatttac gccaatacgt acctggttgc cctttacccc
      781 taaaactgtc acggtgacct catccccaat catgagggtc tcaccaactc gacgagtcag
      841 aatcagcatt ctttgctcct tgaaagatta aaagagtcgg gtctctctgt atcccggcat
      901 tatccatcat ataacgccaa aaagtaagca atgacaaaca cattacatgt aagcagtcat
      961 ggcattacat tctgttaaac ctaagtttag ccgatataca caacttcaac ctgactttat
     1021 cgttgtcgat agcgttaatg cgaatgccgt gaagcgagtc cacggcattg cctgacgctt
     1081 atattattgc aatttcgcgc tgacccagcc tttcacactg gctaacgctg caggtaaggc
     1141 cgcagcatcc gtaccaccgg cttgcgccat gtcaggacgt ccaccaccct tgccgcccac
     1201 ctgctgagcg accataccaa tcagttcccc tgctttcaca cgatctgtga cgtccttaga
     1261 tacgcctgca atcagagaaa ccttaccttc ggctaccgtt gccagcacga taattgtcga
     1321 ccccagctga ttttttaaat cgtcaaccat ggtacgcaac attttcggct caacaccgct
     1381 aagctcgcta accaacagct taacaccatt aacatcaatt gccttactgg aaagatttgc
     1441 gctctcctgt gcggcagctt gttctttaag ctgttgtagt tctttttcta actgacgcgt
     1501 acgttccagt actgagcgca ctttatccgc cagattattg ctatcgcctt tcagcagatg
     1561 cgcgacttcg cttaagcgat cgctgtctgc atgaacggtg gtgatagcgc cttctccggt
     1621 taccgcttcg atacgacgaa cgcctgcagc agtacccgat tcagagatga tgcggaacag
     1681 accaatatca ccagtgcggc tggcgtgagt accgccacac agctcggtgg agaaatcgcc
     1741 catgctcagc acgcgtacac gctcatcata cttctcgccg aacagcgcca tcgcaccttt
     1801 cgctttcgcc gcttcgagat ccatgatgtt ggtttcgatc ggcaaattgc gacgaatctg
     1861 tgcgttcacc aggtcttcga ccgcacgaat ctcttccggt ttcatcgctt cgttgtgtga
     1921 gaagtcgaag cgcagcacct tgtcgttaac cagtgaacct ttctgcgata catgagtccc
     1981 cagaacctgg cgcagcgcag cgtgcatcag gtgcgttgcg gagtgattca gacgaatacg
     2041 ggcgcgacga gcctcatcaa catcagcctg cacagcgtcg cccactttca gagaacccgc
     2101 agcaagttta ccgatgtgac caatcgcctg gccgtatttc tgcgtatctt ccaccgcgaa
     2161 ggagaagtta gcgcctttca gttcgccttt atcaccaacc tgaccgccgg attccgcata
     2221 gaatggtgtt tgatccagca cgaccacagc ttcctggcct gcattgatgg catcaaccgc
     2281 tttaccatca acaaacagcg cggtcacttt gccgttcagt tccagatggt catagccttt
     2341 aaattcagat gcactatcaa cacggatcat tgcgttgtaa tcggcaccaa agccgctggc
     2401 ttcgcgcgcg cgacgacgct gctcttccat tgctgcttca aaaccagctt cgtcaacttt
     2461 gatgttgcgc tcacgacaaa catcagccgt caggtcaacc gggaagccat aggtgtcgta
     2521 cagacggaaa gccgtttcac catccagcgt atcaccagaa agttttgcca gctcttcatc
     2581 cagcaacgcc agaccgcgct ccagagtacg ggcaaactgc tcttcttcag tcttcagcac
     2641 ctgctcaacc tgcgcctgct ggcgtttcag gtcttcaccc gcagagccca taacgtcgat
     2701 cagcggacca accagtttgt agaagaaggt ttctttcgcg ccgagcatat tgccgtgacg
     2761 cactgcgcga cgaatgatac gacgcagtac ataaccacgg ttttcattgg acggcattac
     2821 gccatccgcg atcaggaacg cacaagaacg aatgtggtca gcgattacgc gcagcgattt
     2881 attgctcaga tcggtcgcgc cagtgacttt cgctaccgcc tggatcaacg tgcggaacag
     2941 gtcgatgtca tagttagagt taacgtgttg cagcaccgca gcaatacgct ccagacccat
     3001 accggtatct acagacggct tcggcagcgg ttccatcgtg ccatctgcct ggcggttgaa
     3061 ctgcatgaag acgatgttcc agatctcaat gtagcggtcg ccgtcttctt ccggacttcc
     3121 cggagggcca ccccaaatgt ggtcgccgtg atcgtagaag atttcggtgc acgggccgca
     3181 cggaccagtg tcacccatct gccagaagtt gtcagatgcg tatggcgcac ctttgttatc
     3241 gccgatgcga ataatacgtt cgcgcgggat cccgacttct ttttcccaga tttcgtaggc
     3301 ttcgtcgtcg ctttcataga cggtaaccca cagacgctct ttcggcaggg caaaccattt
     3361 ttcgctggtc agcagttccc atgcaaactg aatggcatcg tgtttgaaat agtcgccgaa
     3421 gctgaagttg cccagcattt cgaagaaggt atggtgacgc gcggtgtaac cgacgttttc
     3481 caggtcgttg tgtttaccac ccgcacgcac gcagcgttgg gaagtggtag cgcgggaata
     3541 attacgcttg tcgagcccaa ggaacacatc cttgaactgg ttcatcccgg cgttggtaaa
     3601 caacaaagtt gggtcgttat ggggtaccag ggagctgctg gcaactacct gatgtccctt
     3661 actatggaaa aagtcgagaa acgcctgacg gatctcagcg gtgctcttgc tcataattat
     3721 cctgaaatca agctaacgaa atatcgccac cagctccagc gtgtcttaac cgccgggctg
     3781 gtaactgaaa agtgggaata agataagttt tcttgactgg gaagtaaaat accgtatgcg
     3841 ttcagtcggc aaaatttcgc caaatatcct ggatatcttc catcagatag ccacgataga
     3901 gcagaaaacg ctggatctta actttttctg aaaagacagt tggcaaaggt tcaccatatt
     3961 ttcgcgtcgc ctgatcgcgc gccagtgcgc accagtcgat gtcacattca cgcatcgctt
     4021 tttctgtcgc ttcgcgggaa atacctttct gattcagttc ctgacgaata cgtgcaggtc
     4081 cataaccttt gcggctacgg ctggcgataa agcgcgcaac aaatcggctg tcatcgagat
     4141 agccatgttc atggcaccag gcaataacgc gctcgtaatc ttctgccgta gcatcaatct
     4201 cttctgggcc atttttgccc ataatcggtg ccacgagttt acgtcgcagt tcttgctcac
     4261 tgtgatcgcg caccgccaga atgcgtaccg cacgatccaa caggcgagca tatgccgggc
     4321 gacgggacgt tgattctgtc atggcatatc cttacaactt aaaaaagcaa aagggccgca
     4381 gatgcgaccc aggttttgct gataaacaga tttaatagtc aatgagtgtg cagaatgttc
     4441 ccgcaggccc aattcgttag aggatcgagg tgagagtaat ttttcacgcc agtgctggcc
     4501 tgttcccctc accctaaccc tctccccaaa ggggcgaggg gaccgtccgt gcacacatta
     4561 tctattatca tcaataacga acgattaaaa atcttcgtta gtttctgcta cgccttcgct
     4621 gtcatctaca gagaaatccg gcgttgagtt cgggttgctc agcagcaact cacgtacttt
     4681 cttctcgatc tctttcgcgg tttccgggtt atctttcagc caggcagttg cattcgcttt
     4741 accctgaccg atcttctcac ctttgtagct gtaccacgcg cctgctttct cgatcagctt
     4801 ctcttttacg cccaggtcaa ccagttcgcc gtagaagttg ataccttcgc cgtagaggat
     4861 ctgaaattca gcctgtttaa acggtgcagc gattttgttc ttcaccactt tcacgcgggt
     4921 ttcgctaccc accacgtttt cgccctcttt caccgcgccg atacgacgga tgtcgagacg
     4981 aacagaggcg tagaatttca gcgcgttacc accggtagtg gtttccgggt taccgaacat
     5041 cacaccaatt ttcatacgga tctggttgat gaagatcagc agcgtgttgg actgcttcag
     5101 gttacccgcc agcttacgca tcgcctggct catcatacgt gccgcaaggc ccatgtgaga
     5161 gtcgccgatt tcgccttcga tttccgcttt cggcgtcagt gccgccacgg agtcaacgac
     5221 gataacgtct actgcgccag aacgcgccag ggcgtcacag atttccagtg cctgctcgcc
     5281 ggtgtccggc tgggagcaca gcaggttatc gatatcgacg cccagtttac gtgcgtagat
     5341 tgggtccagc gcgtgttcag catcgataaa cgcacaggtt ttaccttcac gctgcgctgc
     5401 ggcgatcacc tgcagcgtca gcgtggtttt accggaagat tccggtccgt agatttcgac
     5461 gatacggccc atcggcagac cacctgcccc aagcgcgata tccagtgaaa gcgaaccggt
     5521 agagatggtt tccacatcca tggaacggtc ttcacccagg cgcatgatgg agcctttacc
     5581 aaattgtttc tcaatctggc ccagtgctgc cgccaacgct ttctgtttgt tttcgtcgat
     5641 agccattttt actcctgtca tgccgggtaa taccggatag tcaatatgtt ctgttgaagc
     5701 aattatactg tatgctcata cagtatcaag tgttttgtag aaattgttgc cacaaggtct
     5761 gcaatgcata cgcagtagcc tgacgacgca ccgcatcacg gtcaccgctg aagcattccc
     5821 gccgggtaat gccttcaccg cgggcagtgg caaaagcaaa ccagacggtg ccgacaggct
     5881 tctcttcact gccgccatcc ggcccggcga taccactaat agacacggca taatcagcac
     5941 gagccgcttt cagtgcacct atcgccattt ccaccacgac gggttcactc accgcgccat
     6001 gctgcgccag cgtctcttcg cgtacgccga tcatctgcgc tttggcttcg ttactgtagg
     6061 tgacaaatcc gcgttcaaac caggcggagc taccggcaat atcggtaatc actttcgcta
     6121 cccaaccacc ggtacaagac tcggcagttg ttacggttgc gccacgggct ttcagcgcct
     6181 gcccaacctg ttcacttaac tgcatcagtt cactgtcagt catcacaacc tctgtcagtc
     6241 aaaaatttat gccggacaag atagcacttt cgtcaggaag atggtgttgg cgttgcaatt
     6301 ttacgaggag gattcagaaa aaagctgatt agccagaggg aagctcacgc ccccctcttg
     6361 caaatagtta ctgcacgcgc gccagcgcca cggcttgtcc taactgccag accgccattg
     6421 cgtaatgggt gctgtggtta taacgggtaa tggtgtagaa gttcggcaga ccgtaccagt
     6481 actggtaacc ggtaccgaca tccagacgca gcaggctggc ttgttgatgg ttgcccagcg
     6541 gctgctgtgg cgttaaacct gccgtggcaa gctgcgaaat gctgtacctg gttttgaagc
     6601 catttggcaa gcctggagcc tgaccgtttg ccattaccgc gacctgatcg cctttcaccc
     6661 agccgtgcgc tttgaaatag ttcgccacgc taccgatcgc atcaaccgga tcccacaggt
     6721 tgatatgccc gtcgccgctg aaatctaccg catattgttt gtaagacgac ggcataaact
     6781 gtccgtagcc catcgccccg gcaaaggaac ctttcagatt gagcggatcg tcctgctcgt
     6841 cgcgcgccat caacaggaag gtttccagct cgccagagaa atactccgcg cggcgtgggt
     6901 agttaaatga cagcgttgcc agcgcatcga ggatgcgagt tttccccatc acgcgccccc
     6961 agcgggtttc aacgccgata atcccgacga taatttccgg cggtacgcca tacacctgcc
     7021 acgcgcgatt caacgcatct tcatactgat tccagaaaac cacaccgttc tgcacgttgt
     7081 ccggcgtaat aaattttttg cgataacgga gccatgcgcc gttcggacct gatggcggtt
     7141 tcaccgatgt ggttggtgcc tggttatcca tcagccgcag taccgaatcc agacgcttcg
     7201 cctgggagag aatttcctgc aactgctgac gatcgaaacc gtgtttgttc accattttgt
     7261 cgatgaactg ctgggcattc gggttattag cgaaatcgcc gcccatctgc atcacattgt
     7321 gctgcggctc aagcaggaag ccgccagacg gcgttccggt ggtcgtttca gtctcagtag
     7381 gttttggctt gctgctacag gcggcaagca acacaaaaag gggaagcaat gttacataac
     7441 gacgcttgaa catgaggggt ccatttaaca gattcaacca ggggcaagta tggtaaagca
     7501 tcacgcccca cacaaggaag cggtagtcac tgcccgatac ggactttaca taactcaact
     7561 cattcccctc gctatccttt tactcaaact ttcaaattaa aatatttatc tttcattttg
     7621 agatcaaaat aacactttta aatctttcaa tctgattaga ttaggttgcc gtttggtaat
     7681 aaaacaataa atcctgaagg agagaacaat gatagaaacc attactcatg gtgcagagtg
     7741 gtttatcggg ctgttccaaa agggcggaga ggtgtttacc gggatggtga ccggcattct
     7801 tccgctgttg attagcctgc tggttatcat gaacgcactg attaatttta tcggtcagca
     7861 tcgtattgaa cgttttgctc aacgttgcgc cggtaaccct gtttcccgtt acctactgtt
     7921 accgtgcatt ggcacgtttg tcttttgcaa tccgatgacc ttaagcctgg gtcgctttat
     7981 gccggaaaag tacaaaccca gctactacgc ggcggcctct tatagctgcc actcaatgaa
     8041 tggcctcttc ccccatatca accctggcga actgtttgtt tatcttggca ttgccagcgg
     8101 tctgacaacg ctgaacctgc cacttggccc actggcggtg agttatctgc tggttggtct
     8161 ggtcaccaat ttcttccgcg gctgggtgac cgatctgacc accgccgttt ttgagaaaaa
     8221 gatgggcatt caacttgaac aaaaagttca cctggcagga gcaacatcat gacgcgtatt
     8281 cggatcgaaa aaggaacggg tggctggggc ggcccgcttg agctggaagc cacgccgggc
     8341 aaaaaaatcg tctatatcac cgccggtacc cggcctgcga ttgttgacaa actggcacaa
     8401 cttactggct ggcaggctat tgacggattt aaagaaggtg aacccgcgga ggcggaaatt
     8461 ggtgtcgcgg taatcgactg tggcggcaca ttacgctgcg gcatctatcc gaaacggcgt
     8521 attcccacca ttaatatcca ctcgacgggc aagtccggtc cactggcgca gtacattgtg
     8581 gaagatattt atgtctctgg cgtaaaagaa gaaaacatca ctgtagtggg tgatgcgaca
     8641 ccacaaccct cttccgtggg ccgtgactat gacaccagca agaaaatcac cgaacaaagc
     8701 gatggtttac tggcgaaggt gggaatgggg atgggttctg ccgttgccgt gttgtttcaa
     8761 tctggtcgtg acaccatcga cactgtatta aaaaccattc tgccgtttat ggcattcgtc
     8821 tcggcgctca ttggcatcat tatggcttct ggccttggtg actggattgc ccacggtctt
     8881 gctccgctgg cgagccatcc actgggtctg gtcatgctgg cgctcatctg ctccttccca
     8941 ctgctttcac ctttcctcgg cccaggcgca gttatcgcac aggttatcgg cgtattgatt
     9001 ggcgtgcaga ttggtctcgg caatattccg ccgcatctgg ctttacctgc actgtttgcc
     9061 atcaacgcgc aggcggcctg cgacttcatc ccggtcggtt tgtcgctggc ggaagctcgt
     9121 caggacacgg ttcgcgtcgg tgtcccttct gtactggtga gccgcttttt aaccggcgcg
     9181 ccgactgtac tgatcgcctg gtttgtctcc ggttttatct atcaatagag gctgaaacat
     9241 gaccgttatt tatcagacca ccatcacccg tatcggcgcg agtgccactg acgccctcag
     9301 cgaccagatg ctcatcacct ttcgtgaagg cgcgccagcg gacctcgaag agtattgctt
     9361 cattcattgc cacggcgagt tgaaaggtgc actccatccc ggtttgcaat tttcactcgg
     9421 gcagcatcgc tatccggtga ccgctgttgg cagcgtggcg gaagacaacc ttcgcgaact
     9481 gggtcatgtc accctgcgct tcgatggttt aaacgaagcg gaatttccgg gcactgtcca
     9541 tgtggcaggc cctgtacccg acgatatcgc gccgggatcg gttttgaagt ttgaatctgt
     9601 taaggagtaa aaaatgaatc aggttgccgt tgtcatcggt ggtgggcaaa ccttaggcgc
     9661 gttcctgtgc cacggtctgg ctgccgaggg gtatcgcgtc gcggttgtcg atattcagag
     9721 cgacaaagcc gcaaatgtgg cacaagaaat taacgccgaa tatggtgaag gtatggcgta
     9781 cggttttggt gctgacgcca ccagcgagca aagcgttctg gcgctctctc gtggggtaga
     9841 tgaaatcttt ggtcgcgtgg atttgctggt ctatagcgcc ggaatagcaa aagcagcctt
     9901 tatcagcgac ttccagctcg gcgattttga ccgttcgcta caagtgaatc tggtgggtta
     9961 tttcctgtgt gcccgtgaat tttcgcgttt gatgatccgc gacgggattc agggtcgcat
    10021 tattcagatc aactcgaaat ccggcaaagt gggtagcaaa cacaactctg gctacagcgc
    10081 agcgaaattt ggtggcgtcg ggctgactca atcactggcg ctggatctgg cggagtacgg
    10141 cattacggtg cattcactga tgctcggtaa cctgctgaaa tcgccgatgt tccagtcact
    10201 gttgccacaa tacgcgacca agctgggtat caaaccggat caagtcgagc agtattacat
    10261 cgacaaagta ccgctcaaac gcggctgcga ttatcaagat gtgctgaata tgctgctgtt
    10321 ctacgccagt cctaaggcgt cgtactgcac cggacagtcg atcaatgtca ccggcggtca
    10381 ggtgatgttc tgatcaacag cggagatccg ttaaggatct ccgtgagact atagaatgcc
    10441 tgatgcgcta cgctcatcag gcatacagga cttccgccac tacattaagg aaaagttatg
    10501 gtatccgcac tcatcaccgt cgccgttatc gcctggtgtg cgcaactggc cttaggcggc
    10561 tggcaaattt ctcgttttaa ccgtgccttc gacacactat gccagcaagg gcgggttggc
    10621 gtgggccgtt ccagcgggcg ctttaaaccg cgggtcgtgg tcgccatcgc cctggacgat
    10681 cagcagcgca tcgtcgacac cttgtttatg aaaggactga ccgtcttcgc ccgaccgcaa
    10741 aaaattcccg caattaccgg tatgcatgcg ggtgatttac agcccgatgt gatctttccc
    10801 catgatccac tatcacagaa tgctctatca ttggcgctta aactgaaacg tggataattt
    10861 cgttgtgaat gttacttgct tgcgaagtta tcattttgaa acctaaatca ggtaatcacg
    10921 cccatgaaac ctcgtcagcg tcaggccgcc attctggagt atctgcaaaa gcagggtaaa
    10981 tgctcggttg aagaattggc gcaatacttt gacaccacag gcacaaccat tcgcaaagat
    11041 ctggtcattc tggaacatgc cggaaccgtc attcgtactt atggcggagt ggtgttgaat
    11101 aaagaggaat ccgatccgcc tatcgatcat aaaacgctca tcaacaccca caagaaagag
    11161 ctgattgcag aagctgccgt tagttttatt catgatggcg actcaataat tcttgatgct
    11221 ggcagtaccg ttttgcagat ggtccccctg ctctcgcgct ttaataacat cacggtgatg
    11281 accaacagtc tgcatatcgt caatgcgcta tctgaactgg ataacgaaca aaccatcctg
    11341 atgccaggcg gaacatttcg caaaaaatcg gcctcatttc acgggcaact ggcagagaat
    11401 gccttcgagc atttcacctt cgacaaatta ttcatgggta ctgacggcat cgatctcaat
    11461 gcgggcgtaa ccaccttcaa cgaggtctat accgtcagta aggcaatgtg caatgccgcg
    11521 cgcgaagtga ttttaatggc ggactcatca aagtttggcc gtaaaagccc caacgtagtt
    11581 tgcagtcttg aaagcgtcga taagctgatt accgacgcag gtatcgatcc ggcgtttcgt
    11641 caggcgctgg aagagaaagg gatcgatgtg atcataaccg gagagagcaa tgagtgaagc
    11701 actactgaac gcgggacgtc agacgttaat gctggagttg caggaagcaa gccgtttacc
    11761 ggaacgtctg ggcgatgatt ttgttcgcgc cgccaatatc atcctgcact gcgaaggcaa
    11821 agtggtggtt tcgggaattg gcaaatcggg ccacattggt aagaaaatcg ccgcaacgct
    11881 tgccagtacc ggcacaccgg ctttttttgt ccatccggca gaagcactgc acggcgatct
    11941 ggggatgatt gaaagccgcg atgtgatgct gtttatctct tactccggtg gcgcgaagga
    12001 actggatctg attattccgc gtctggaaga taaatctatc gcgctgctgg cgatgaccgg
    12061 caaaccgacg tcaccgctgg gcctggcggc aaaagcggtg ctggatatct ccgtagaacg
    12121 cgaagcctgc ccgatgcacc ttgcgccgac ctccagcacc gtcaataccc tgatgatggg
    12181 tgacgcgctg gcgatggcgg tcatgcaggc gcgcggattt aatgaagaag attttgcccg
    12241 ctcccaccca gccggggcac tgggcgctcg cttgctgaat aaagtgcatc atctgatgcg
    12301 ccgtgatgac gctatcccgc aggtagcgtt aaccgccagc gtgatggatg cgatgctgga
    12361 actcagtcgc accggtttgg ggctggtggc agtatgtgac gctcaacaac aggtacaagg
    12421 cgtctttacc gacggcgatt tacgtcgctg gctggttggc ggcggcgcac tcaccacgcc
    12481 agttaatgaa gcgatgacga caggcggcac cacattacag gcacaaagtc gcgccatcga
    12541 cgccaaagag atcctgatga agcgcaaaat cactgccgca ccggtggtgg atgaaaacgg
    12601 caaactcacc ggcgcaatta acctgcagga tttctatcag gccgggatta tttaatcctt
    12661 caatcccaga cgtttcgcca gccgatgcag gttggcgacg tcggtttcca gcatccgcgc
    12721 gcaggcagcc cagttgtgat gattttgtgc cagtgcctgg cgaatggttt cacgctggaa
    12781 cgcttctgtc gcttcacgca ggttttgctt aacaacgggc accgccgccg cttctggcgg
    12841 cggcaacgtc acctcaggaa aagcaaaatg ttgcgcctca agaatcactt catcgccgtt
    12901 gcgggtggct ctcgacagca ctaccgcccg atgaatagca tgttccagtt cgcgcacgtt
    12961 ccccggaaaa cgatagtgtt gcagtaaatt tcgcgctccg gcacttaata ccacgcggga
    13021 gagccccagc cgcaaacgac actgctcgca gaaatacccc gccagcagaa tgacatcatc
    13081 gccccgctca cgcagcggcg gcaccgaaag tggaaacacg ctcaggcgat gaaacaagtc
    13141 agcgcgaaat cgccctgcca gcacctcttc gcgtaagtcg cggttagtcg ccgccagcac
    13201 gcgcacatcg acccgcaaac tgcggtcatc gccaacgcgc tgaatatcgc catactgcaa
    13261 caccctcagc agcttggcct gcaatgccaa cgacaactcg ccgatctcat ccagaaacag
    13321 agtgccgtta tccgccattt caaacttccc gctgcggtta ctgatagcgc cagtaaacgc
    13381 tcctttcaca tgcccgaaca actcactttc cgccacactt tccggcagtg cagcacagtt
    13441 gagatagacc agcggattca ccgcccgtgg cgaggcttca tgaatcgctt tcgccaccag
    13501 ctccttaccg gttcccgtct caccgctgat caggacgttg agatcggacg ccgccacaat
    13561 ctcaatctct tttttcagtt gcgtcatgcc aggggacaag ccaatcatct gcgtctgttt
    13621 caccgcttca aacggcgtgg catcgcctgg catcatattc tggctttcca gttgttcaat
    13681 cagcaacgca ttgcttaacg ctcccgccgc cagcgcagca atcagccgta gctcttcgtc
    13741 gctgaaaaca tcgaactgat cgggctgcat cccgtcgagc gtcagtgcgc cgatcaggtt
    13801 ttgcccggca aacaatggca gaccaacgca ggcgtgaacc ttcagactct cctgcccagg
    13861 aatcaaaccg tcatagggat cgggcaattc gctgtctgcg ggaaagcgca ccacatcccc
    13921 ggcgcgggca atcgcttcca gccgtggatg cccttccagc gcaaagcgtc taccgagtac
    13981 atcctttgcc agaccgtcga tggcaagcgg aataaactgc cgcgaatcgt aacgtagcaa
    14041 cgcagacgca tcgcactcca gcacctgacg tagcgtggtg atcaggcgct gaaaacgatc
    14101 ctgatgacca atcccacgct gcaattcgat ggcgatattc gccagcacat caacggaaaa
    14161 actcatcttt gcctcactgt caatttgact atagatattg tcatatcgac catttgattg
    14221 atagtcattt tgactactca ttaatgagca taattttatt tatatagtaa aaacaatcag
    14281 ataaaaaact ggcacgtaat ctgcaattag caagacatct ttttagaaca cgctgaataa
    14341 attgaggttg ctatgtctat tgtggtgaaa aataacattc attgggttgg tcaacgtgac
    14401 tgggaagtgc gtgattttca cggtacggaa tataaaacgc tgcgcggcag cagttacaac
    14461 agctacctca tccgcgaaga aaaaaacgtg ctgatcgaca ccgtcgacca taaattcagc
    14521 cgcgaatttg tgcagaacct gcgtaatgaa atcgatctgg cggatatcga ttacatcgtg
    14581 attaaccatg cagaagagga ccacgccggg gcgctgaccg aactgatggc acaaattccc
    14641 gatacgccga tctactgtac agccaacgct atcgactcga taaatggtca tcaccatcat
    14701 ccggagtgga attttaatgt ggtgaaaact ggcgacacgc tggatatcgg caacggcaaa
    14761 cagctcattt ttgtcgaaac accaatgctg cactggccgg acagcatgat gacttacctg
    14821 acaggcgacg cggtgctgtt cagtaacgat gctttcggtc agcactactg cgacgagcat
    14881 ctgttcaacg atgaagtgga tcagacggag cttttcgagc agtgccagcg ttactacgcc
    14941 aatatcctga cgccgttcag ccgcctggta acaccgaaaa ttaccgagat cctgggcttt
    15001 aacttaccag tcgatatgat agccacttcc cacggcgtgg tatggcgcga taacccgacg
    15061 caaattgtcg agctgtacct gaaatgggcg gctgattatc aggaagacag aatcaccatt
    15121 ttctacgaca ccatgtcgaa taacacccgc atgatggctg acgctatcgc ccaggggatt
    15181 gcggaaaccg acccacgcgt ggcggtgaaa attttcaacg tcgcccgaag cgataaaaac
    15241 gaaatcctga ctaatgtctt ccgctcaaaa ggcgtgctgg tcggcacttc gacgatgaat
    15301 aacgtgatga tgccgaaaat cgccgggctg gtggaggaga tgactggttt acgcttccgt
    15361 aacaaacgcg ccagtgcttt cggctctcac ggctggagcg gcggtgcggt ggatcgtctt
    15421 tccacgcgcc tgcaggatgc gggtttcgaa atgtcgctta gcctgaaagc gaaatggcga
    15481 ccagaccagg acgctctgaa gttatgccgt gaacacggtc gcgaaatcgc ccgtcagtgg
    15541 gcgctcgcgc cgctgccgca gagcacggtg aatacggtag ttaaagaaga aacctctgcc
    15601 accacgacgg ctgacctcgg cccacggatg cagtgcagcg tctgccagtg gatttacgat
    15661 ccggcaaaag gcgagccaat gcaggacgtt gcgccaggaa cgccgtggag tgaagtcccg
    15721 gataacttcc tctgcccgga atgctccctc ggcaaagacg tctttgaaga actggcatcg
    15781 gaggcaaaat gagtaacggc attgtgatca tcggttcggg cttcgccgcc cgccaactgg
    15841 tgaaaaatat tcgcaaacag gacgccacta ttccattaac cctgattgcc gccgacagca
    15901 tggatgagta caacaaacct gacctcagcc atgttatcag tcaggggcaa cgtgccgatg
    15961 accttacccg ccagacggcg ggtgaatttg ccgagcagtt taatctgcac ctgtttccac
    16021 aaacctgggt gacggatatc gatgccgaag cccgtgtggt gaaaagccag aataatcagt
    16081 ggcaatacga caagctagta ctggcaaccg gtgccagtgc ctttgtcccg cctgtgcctg
    16141 ggcgtgagtt aatgctgacg ttaaatagtc agcaagagta tcgcgcctgt gaaacgcaac
    16201 tgcgggatgc ccgacgcgtg ttgattgttg gcggtggttt gattggtagc gaactggcga
    16261 tggatttttg tcgtgcaggc aaagcggtca cgctaatcga caacgctgcc agtattctgg
    16321 cgtcgttaat gccaccggaa gtaagcagcc gcttgcagca tcggttgacg gagatgggcg
    16381 ttcatctgct gttgaaatct cagttacagg ggctggaaaa aacggattct ggcattcagg
    16441 caacgctgga ccgccagcgc aatatcgaag tggatgcggt aattgccgcc accggactgc
    16501 gcccggaaac cgccctggca cgacgcgccg ggctgacgat taatcgcggc gtttgcgtcg
    16561 atagttatct gcaaaccagt aataccgata tttacgcgct gggcgattgc gcggaaatta
    16621 acggtcaggt attgccgttc ctccagccga ttcaacttag cgcgatggtg ctggcaaaaa
    16681 atcttctcgg caataacacg ccgctgaaac tcccggcgat gctggtgaaa atcaaaacgc
    16741 cggaattacc gctgcatctg gcaggcgaaa cccagcgtca ggatttacgc tggcaaatta
    16801 ataccgaacg ccagggaatg gtggcgcgcg gcgttgacga tgcagaccag cttcgcgcct
    16861 ttgtggtcag tgaggatcgg atgaaagagg catttggatt gttgaaaaca ttgccgatgt
    16921 aggtgggcta ctgtgcctaa aatgtcggat gcgacgctgg cgcgtcttat ccgacctacg
    16981 gggacgcatg tgtaggccgg ataaggcgtt tacgccgcat ccggcaatgg tgtccaaatg
    17041 caacacgttt tatccgttct ggacttcacc cgctaaccaa cgcgccgcag caataacccc
    17101 ctgccccaga gacaaaccgc catcacccgc cggtaaactc tgtggaaaga gcaatgtgaa
    17161 atcagcgaga taatgcgcca gacgtgcacg cagcaaacgg ttatgaataa ccccgccgct
    17221 aaataccagc gtagtgatac cacgcatcgt ggcctgctca cgcatcaacg cggcaaaacc
    17281 ctgcgccagc gcatcatgaa acgcccacgc gcgttgatta accggtgcct gccagttcag
    17341 ccactgctgc cagaaagtgg cgagatccag ttgattgtcc acccgcggca ttgtcaccgg
    17401 atgcgtcact ccgtggcatg aggctgcgag cgcctccaga gcacaagccg cttcaccttc
    17461 ataacttaac gtggctggcg cacagcccag tgccgccgcc actgcatcga aaaaacgccc
    17521 acacgatgac gccagcggcg cgttaattcc acgctcaatg gcccgcgcca gcacgctcca
    17581 gttttgctgt tgcacactcg ctgtttcggg ataattctgc cactccggca caaagcgcag
    17641 gcactgcgcc agcaggtttc gccacggctg cttcgccgcc aaatcgccac cagcaagcgc
    17701 cactgcaggc aagccgccca ggtgctcaca ttcgcgatag ttcacccgca ggcactcgcc
    17761 gccccacaaa gcgccattct cccccatacc gataccgtcg agcgtcaaag caatgacatc
    17821 accgccatcc agcggccact gatgctctgc cagacacgcc gctgcgtggg catgatgatg
    17881 cagcaccgtt tgcgtcggca gattcatttc gtgcgcccac tggctggaga catagcccgg
    17941 atgtgcgtca tgcacaacgt attgcggggt gaaatcgtag atgttttgca tcaggcgtaa
    18001 cgcttcgcgc cactgcatct ggatgccatc gtcacttaaa tcgcccagat gctgactcaa
    18061 caccgcttgt tcaccgcgca ccaggcagaa ggtatttttc agatccgcgc cgagacacag
    18121 cacaggcgga acatttttaa agcccggagg caaagccagc gcatccggca catacccgcg
    18181 tgaacggcgc agcatttcgc cgctttcgcg caccactgaa tcatccatcc gctgcacgat
    18241 gtcgcggtta tgtattaaga atccgtcggc aatgccctgc aaatccgcca gcgcctgttc
    18301 gttgctgata gccggtggtt taccgctcag gttgccggag gtcatcacca gcgggcattg
    18361 cagttcctgt aacagcaaat gctggagcgg gttcgcaggc aacatcaccc cgacttcgtt
    18421 aaggccaggg gcgatatcat cacaaagctc aggaacgtat tttttatcca ccagcacaat
    18481 cggcgcggcg ggcgtggtaa gcaactggcg cgcagcgtct ggtaaaccgt cagccactgg
    18541 caacatgacc gccagcggtt tcgccggacg atgtttgcgc gcccgaagtg tcgccaccgc
    18601 gttactgtta cgtgcatcgc aggcaagatg aaatccgcca atccctttga tggcgacaat
    18661 gttgcccatt tttaactgtg cgatagccgc ctgtaatgcc gcctcttgtt ctgcatgttc
    18721 accatgactt acccattcaa gatgtgggcc acactccggg caggccaccg gctgggcgtg
    18781 gaagcgacga tcgagcgggt cgcggtactc tttatcacag gctggacaca gcggaaactc
    18841 cgccattacg gtaaacgggc ggtcgtaagg catggcgcga ataatggtga aacgcgggcc
    18901 gcagtgggta cagttgataa acggataacg ataacgccgt tcgcctgggg tattcatttc
    18961 ggcaaggcaa gcagggcaag tagcggcatc cgggacaatt tgcgtattca tggcacctcc
    19021 cgcgctctgg cggatagtga actcggtggg cagttgtgac cagataaacg gctcacgctc
    19081 gacgctatca atacgcgcca gtggcggaca gtgctgatgc aattgaacaa gaaacgtttc
    19141 cgggtcttcc agcagccgga cttctacgcc atcgccgtca ttacagacat cgccgtgaag
    19201 atttaattgc tgtgccagct gccagacaaa cggacgaaaa ccgacgccct gcactttgcc
    19261 tcgaatacgc agttggacac cgcaagatgt gttttttgcc attgagttat tcccgccatc
    19321 atgaattgcg taacccgccc tgccggacac gactgcgtcg tatccggcag tctcaggtcg
    19381 gcgataccgc cagctccgta ttctacgaat atttccggga attcctttga tgccagaaca
    19441 gttctataag atttttagaa catcagcgcc gtacggcgac gtttttctgc gctcagttgt
    19501 tcaagtttat tacgatcgac acaaatcagc gcatgggtcg ggcaagccgc catacacgcc
    19561 gggccgtctt cgcgatggtt gcacaggtcg catttattgg cttcagcttt gtcagcccgt
    19621 acattcagcc ccgcgccgct gttgcggatc accggacgta ccaccacttc catcgcgcca
    19681 tacgggcaag ccaccacgca ggttttgcaa ccaatgcaac gttcctgcat cacatgaaca
    19741 aaccctttat cacggctgat agcaccattc gggcagacgt tagcgcacgg tgcatcttca
    19801 cactgacggc aaactgtcgc cgtggaaatg ttcacacctt taatgacatg gatacgcggt
    19861 aaaaatgttt ccggggtcag cgatgcacag tcctgatttt cctgatgaga aaccacgcac
    19921 gccacttcac aggtacggca accaatacat ttactcgcgt cagcaatgat gaaacggttc
    19981 atcaaattct ccagcaatga cagttaatgc gccgatacat tcacaaatca tgccagtttt
    20041 taattcactg ttatttaagg aaattaattt ctgtaatgca ggaaaaaaga tgtcatcgac
    20101 actagtgacg atgacatgtg atgacaatgt ttatcgcgaa agagcaatga gtgagtcgcg
    20161 gcggatcagt tttccgctga aggttttcgg cggtgagaaa tccccgccat cgagcataaa
    20221 aatcagccgt ccaataattt cctgaatcat ctcagtcacc ggaattttta cgctggagag
    20281 cgccggaacg gtgtaggggg caatagcgat atcatcgaat ccgataactg acacctgctc
    20341 tggcaccgct acgccgcgct cgtgtaacgc tttcatcgca cctatcgcca tatcgtcgtt
    20401 actggcaact aacgcgctaa atttagcccc acgttcgagc aacatttcta ccccttcggc
    20461 cccgctggca ggcgtccatt taccgttagc gataagtttt tcattgagcg caataccatg
    20521 ctgcgccagc gcgtctttat acccggcaag acgttcaatg ctggtggggg aatccatcga
    20581 gccggtaagg aaagcaatct cctgatgccc ggcgtttatc aactctgcca cggcgttaaa
    20641 gctggtctgt ttatgatcgc accagacgct atggctgcta tttttgcgca ggcggcgatt
    20701 aagcaccatt atcggctgac tgtgcgcgtc aatgatgtca tcaatctcat ccacgcttaa
    20761 aaaacgcggg taaatcataa tggcgtcgca gcgcaaatcc agcagatact gaatcgcctg
    20821 gcgctcttct tcggcgctgt gtttaccatc tgccaatagc aactgtcgcc ctttctcttc
    20881 cgccattcgc gcggcatgaa acagtaattc actaaaataa atgccgtgat aaagcgtgtt
    20941 ggtcactacc agccccagcg tctgagtact cttcgccgac agattgcggg ccagcaaatt
    21001 tgggcggtaa ccgctctctt ctaccgcctg aaacacgcga tctttagtct cctggctgac
    21061 gtagccatta cctgaaagca cgcgggaaac ggtcgctttt gaaaccccgg cgcgcttcgc
    21121 cacttccagc atcgtcgtca tcattttcat ccctttacac gcaatcaacg cagtgtactg
    21181 caccgtttgc cgattgtcct tgcgcaatcg gcgggaaaaa tattcaggtg accggtttca
    21241 caaatataaa aaatgaacaa ttaactctct tgcttattta gtgacaacta ttcatgattt
    21301 tgtgaaaccg gtttcctaat tccgtttcag catcggcatt tttccgttac gtcgactgat
    21361 aacaactaca tctaccctac tgataacagg ataaaatccg atggccaaaa attatgcggc
    21421 gctggcacgc tcggtgatag cggcactggg cggcgttgat aacatctcgg cggtcacgca
    21481 ctgtatgacg cgtttgcgct ttgttatcaa agatgaccag cttatcgaca gcccgacgtt
    21541 aaaaaccatc cccggcgtgc tcggcgtggt acgtagtgac aaccagtgtc aggtgattat
    21601 cggcaatacc gtttcacaag cctttcagga agtcgtcagc ctgctgccgg gagatatgca
    21661 gcccgcacag cccgtgggta aacccaaact cacgctgcgt cgcattggtg cagggatcct
    21721 cgatgcgctg atcggcacca tgtcaccgct gatcccggcg attatcggcg gatcgatggt
    21781 caaactgctg gcgatgatcc tcgagatgag cggcgtgctg acaaaaggat cgccgacctt
    21841 aaccattctg aatgtgattg gtgacggtgc tttcttcttc ctgccgctga tggtcgcggc
    21901 atctgccgcc atcaaattta aaaccaatat gtcgctggcg attgccatcg cgggtgtgct
    21961 ggtacatccg agttttattg aactgatggc gaaagcggcc cagggtgaac atgtcgaatt
    22021 tgccctgatt ccggtcaccg cggtgaaata tacctacacg gtgatcccgg cgctggtgat
    22081 gacctggtgc ctgtcatata tcgaacgctg ggtggatcgc attacaccgg cggtgaccaa
    22141 aaacttcctc aaaccaatgc tgattgtgtt gattgccgca ccgctggcaa tcctgctgat
    22201 tggcccgatt ggtatctgga tcggtagcgc catttcggcg ctggtttaca ccattcatgg
    22261 ttatctgggc tggctttcag tcgccattat gggcgcgctg tggcctctgc tggtaatgac
    22321 cgggatgcac cgcgtcttta cgccaaccat cattcagacc attgccgaaa ccggcaaaga
    22381 agggatggtc atgccgtcag agatcggcgc taacctgtcg ctgggcggtt catcactggc
    22441 agtggcgtgg aaaacgaaaa acccggaact gcgtcagacg gcgctggctg cggcggcatc
    22501 agccattatg gcggggattt ccgaaccggc gttatatggc gtggcgatcc gcctgaaacg
    22561 tccgcttatc gccagtctta tcagcggttt tatttgcggc gcggttgccg gtatggcggg
    22621 gcttgccagc cactcaatgg cagcgccggg gctatttacc agcgtgcagt tcttcgatcc
    22681 ggcgaatcca atgagcatcg tctgggtgtt cgcggtcatg gcgctggcgg tggtgctgtc
    22741 gtttatcctc acactgttgc tcggctttga ggatattcct gttgaggaag cggctgccca
    22801 ggcgcgaaag tatcagagcg tacaaccgac cgtcgccaaa gaagtaagtc ttaattgagg
    22861 atgaaaatgt cagtatttcc agaaagtttt ttatggggcg gcgcgcttgc cgccaaccag
    22921 tctgaaggtg cgttccgtga aggtgacaaa ggtctgacca ctgtcgatat gatcccacac
    22981 ggcgagcatc gaatggcggt gaaactgggg ctggaaaaac gttttcagtt gcgagatgac
    23041 gagttttatc ccagccatga ggcgacggat ttttatcatc gttataaaga agatatcgcc
    23101 ctgatggcag agatgggatt caaggttttc cgtacctcaa ttgcctggag ccgtctcttt
    23161 ccgcagggcg atgaaatcac gcccaatcag cagggcattg ctttttatcg ttctgtcttt
    23221 gaagagtgta aaaagtacgg tatcgaaccg ctggtcacgt tgtgccactt cgatgtgccg
    23281 atgcatctgg tcaccgaata tggctcctgg cgtaaccgca agctggtgga gtttttcagc
    23341 cgctacgcca gaacctgctt tgaagcattt gatggtctgg tgaaatactg gctaaccttc
    23401 aatgaaatca acattatgtt gcatagcccg ttctccggcg cgggtctggt gtttgaagaa
    23461 ggtgaaaatc aggatcaggt gaaatatcag gccgcgcatc accagctggt tgccagtgcg
    23521 ctagccacca aaatcgccca tgaggttaac ccgcaaaatc aggtgggctg tatgctggcg
    23581 ggcggtaact tctaccctta cagttgcaag ccggaagatg tctgggcggc gctggagaaa
    23641 gaccgggaaa acctgttttt tatcgatgtg caggcgcggg gcacgtatcc ggcttactct
    23701 gcccgcgtat tccgcgaaaa aggggtaacc atcaacaaag caccgggcga tgatgaaatc
    23761 ctgaaaaaca ccgtcgattt tgtctctttc agctattacg cctcgcgctg cgcctcggcg
    23821 gagatgaacg ccaacaacag cagtgcggcg aacgtggtga aatcgctgcg taatccgtat
    23881 ctacaggtga gcgactgggg ctggggaatt gatccactcg gtctgcgtat caccatgaat
    23941 atgatgtacg atcgttatca gaagccgctg tttctggtgg aaaacggcct gggcgcaaaa
    24001 gatgaatttg ctgccaatgg cgagattaac gacgactatc gcatcagcta cttacgcgaa
    24061 catatccgcg caatgggcga agcgattgca gacggcattc cgctgatggg ctacaccaca
    24121 tggggctgta ttgatttagt ttccgcctct acgggtgaaa tgagcaaacg ctacggcttt
    24181 gtctttgttg accgtgacga cgcaggcaac ggtacgctga cgcgcacgcg taagaaatca
    24241 ttctggtggt ataaaaaagt gattgccagt aatggggaag atttagagta ggtaacagtg
    24301 ccggatgcgg cgtgaacgcc ttatccggcc tacatgtcgc acgaaccatg aaggcccgat
    24361 aagcgcagcg catcgggcaa tctggggttt ggcatcagtc aaaatcaaag aaaccattcc
    24421 ggcttaacgc caaatcgggc tgaaagtgcc ttaatgtggg atatcgtcag ggatcgttgt
    24481 ccagacaaaa tctgactgac cagggattta gagccgattt cgttcttcaa atccgcatag
    24541 gtcagattat gttgatcaat caacgtgcga agtaacgcca cgccaaccgg cattgccgca
    24601 acggctttgt cgaactctgc aaacttttcg ttgttgtttt catactctgc aatgcggctc
    24661 gccagaaagt cgataagtgg attggtgtca tcttcttcaa tcaaatattc gaccagttcc
    24721 agtgcttcac gataatcatc ttctgagtca ctgcctccca gaaaggggac tgcattgacc
    24781 agttctcttg ttgctttaac agcgcgtgca gcattagcag tcattctttg ttctctctgt
    24841 agtaacgtgt cagtttgtca tactctgcgt gattcgttat gtgcttcaca aagaaccgct
    24901 tattaacaaa gtttatgtag gcaataaccc taagattgtt cccgccgaca tccagaacgt
    24961 accatttgtt gcgatatcta aaattgtcga ggttgggaaa cgccgttcgc atttcttctg
    25021 gtgtagaaaa atccgtttcc ttaataaccc gatacaatgc ctgaagagcc agagcgtcgt
    25081 taggatattt acgcgcagac tcttcaaatg gcgctttaga aatgatgtgc atttttaagt
    25141 tttaacttcc tgatagtaag taaacagcca catctgcgat gttctatcct tatgtgttcg
    25201 gaaactatag ctcatcaaat ccaagttcac aatatgtaaa cacttgtgcg cgatataatt
    25261 acccccaaaa tcctcatagc cactctgcaa aaagtcagtt ctggttcacc cctcaagcac
    25321 atcccccttt atcaagcaac gctgcgtctg cgccagatct tccgtttgcg ggtcgcgccc
    25381 ggcgatgggt ttgaggacga gtttacggtt gggggtataa gtaaaacgcg tgagcgcggg
    25441 atagcttaac ttgccaaacc agccgtgaac acgctgccat ttggcgtctt ttacctgata
    25501 cagactggtt atccagtaac cagaattaaa ctgcatatcc agcaattgcg tgtgaccatt
    25561 gccttgcaaa tccagcaaat catctacgcc agttttgctt gcggtgtaga atcccctggg
    25621 ttcaaagaca cagggacggc cttcgcgcgt gaacgtcata agaattaaat gtgcataagg
    25681 agccagacca ttaccggtac ttggtaacca gataaccaga tcctgaatgc cgttacgatc
    25741 aagatcggcg cgatagagtt gccctttgcc cgctatgttt gccagcatac ccacaggcac
    25801 agaccaggat ttaccggcgc gatctttacc gttcaattgc cattcactat catcatttgg
    25861 catcttttca agcgtcacgc cagcaacgcc cacttttcta aaatgccagg tttgctcggc
    25921 atcataaggc atggtcagga gaggtttcag cgcccgcttt tgcgcgtatt cgctgtcact
    25981 cattggcaga tattcggtaa agctggtggc ctgtgcaggc agcactaagc tcaataaaaa
    26041 agcgcacatc cctgttctga tattcatttc gctgaattcc ctgatggaga attattcttc
    26101 ttccaccgct aactgcgcga agccgccatt cccttcccag ccttccagtc gttgataaac
    26161 ggtttcaacc gcatctttaa tcggctgggt catcgggtag taaaagccga cgatatccgg
    26221 ctggatgccg aggaaaatca cttcgccaat atcttctttc aactggtcga taaggtagtt
    26281 caacggcatg ttatgggtag tcatcataaa catctcggcg atatcatccg ggtcgatgat
    26341 gcggatctcg ccggggttta gccccatatc agtggcgtcg acaatcagca gtcgcgtagg
    26401 gcgcagttca cggatggcga cgatgtcgtt ttccggtgca ctaccgccgt caatcaccac
    26461 ccagttacct ttcggcgcgg cggcgcactt ttccgccagc agcgggcctg cgccatcatc
    26521 gcccatcatg ctattgccaa cacagagtaa aacgtcagtc acgcagtctc ctcaccatca
    26581 aatagatggc gttttcctga tgaatatcat gcagcatact tagcagcatt gtactccatt
    26641 cctgctgctg cgaagtttgc gctgcccggg ctttatcgaa tgcgttggcc agcatcggca
    26701 catggttaat gtcgatgacg atctcaccat atttcggcac gccttccatt ttgcggcggg
    26761 cttcgctgcc cgcctccaga gtggcaatcc atgccagata ttcatcccac gggcaggtga
    26821 gcgccgcttc caggcaatcg ataaccccaa ggtggtgacc aatcgccagg ctgtaataga
    26881 ccacctgctg cgcctcggcg ggcgtggcat cgttctcatc aataaattta c
//
LOCUS       DAMZET010000046        26647 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.046, whole genome shotgun sequence.
ACCESSION   DAMZET010000046 DAMZET010000000
VERSION     DAMZET010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.046"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1786)
                     /locus_tag="POB34_002042"
     CDS             complement(<1..1786)
                     /locus_tag="POB34_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001471825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HDI5918990.1"
                     /translation="MSHSFQEQYTCVPPSAFAAGTGKAFTGAYAWNTPREAVGRERPL
                     TRDEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPLHGFYFLTSTFQRRLWPRI
                     ERVNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELCDAWV
                     DAHGEKESLFTDEAQAHLYGHVAGAARAFNISPLFWKKYRKGQMTTRQAYSAIARLFN
                     DEWWISQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDL
                     ENRETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKY
                     HPTRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVV
                     EPHHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAA
                     GYIAKYISKNIDGYALDGQLDNDTGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAY
                     RELRKLPSGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPS
                     DEVNEYEEEVERVVGIYAPHLGARHIHIT"
     gene            complement(1783..2790)
                     /locus_tag="POB34_002043"
     CDS             complement(1783..2790)
                     /locus_tag="POB34_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HDI5918991.1"
                     /translation="MITDARCHTENTINVIFVSGGKDSLAQWLVAIEENVPILPVFAD
                     TGHEHPQTMKYLDYLEKRLGPIKRVRADFSCRIESKRKFIQTKWPDLLVNDCGMSEEE
                     AHQRISCALEILKPTGIPFLDLCLWKGRFPSTKARFCTFELKHEPVRSQVILPLLNEF
                     EEVISWQGVRAQESPSRAALPVWEEDADNTPGLHVYRPILHWKHEDVFAIARRHDIKP
                     NPLYQQGCGRVGCMPCIHVRKSELAEIFRRWPEEIKRVAEWERLVASCSRRGNSTFFP
                     STHDPLRAERRIEVVTVEAYGIETYHDWTMTTRGGTQFDLLASANDKAVCSSVYAGVC
                     E"
     gene            complement(2787..3065)
                     /locus_tag="POB34_002044"
     CDS             complement(2787..3065)
                     /locus_tag="POB34_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HDI5918992.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNTQVEQLSKRCSEAFSRGASE"
     gene            complement(3062..3286)
                     /locus_tag="POB34_002045"
     CDS             complement(3062..3286)
                     /locus_tag="POB34_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HDI5918993.1"
                     /translation="MPDNVDFIQEQQAELLERQINAARVKHCGVSALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRTA"
     gene            complement(3286..3588)
                     /locus_tag="POB34_002046"
     CDS             complement(3286..3588)
                     /locus_tag="POB34_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HDI5918994.1"
                     /translation="MSCSHSVVLLNNALKIAVMENGDLSLIQLCLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTEIASYCADEFKKLNDKRSW"
     gene            complement(3588..3812)
                     /locus_tag="POB34_002047"
     CDS             complement(3588..3812)
                     /locus_tag="POB34_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HDI5918995.1"
                     /translation="MHTVSENQCGKYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(3876..4376)
                     /locus_tag="POB34_002048"
     CDS             complement(3876..4376)
                     /locus_tag="POB34_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HDI5918996.1"
                     /translation="MTVMTLNLVEKQPATMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHAGLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(4373..4543)
                     /locus_tag="POB34_002049"
     CDS             complement(4373..4543)
                     /locus_tag="POB34_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918997.1"
                     /translation="MNEPRCIAQLLRNESPRAIDFTITHGKGRKGIIIRTKKQSPLKK
                     ALTFLKSRRVWK"
     gene            complement(4546..4818)
                     /locus_tag="POB34_002050"
     CDS             complement(4546..4818)
                     /locus_tag="POB34_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5918998.1"
                     /translation="MDAENYVIQYPLDAVHVDKFADLLGKPKTAVSEMVKANKLPIIE
                     LRDPCKPKARAGEKWVFIPEFNRAVREAFYNRPVEQRDAWLLWMGL"
     gene            4955..5248
                     /locus_tag="POB34_002051"
     CDS             4955..5248
                     /locus_tag="POB34_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5918999.1"
                     /translation="MKLSEKIKALREAEGLSQSKFCEIIELPLSTLKKYEGGNFEPGG
                     TALLKITMHPTFQKYALWLMTDKTAPDAGQIAPALAHIGPESTESNHSAKRIG"
     gene            5318..6298
                     /locus_tag="POB34_002052"
     CDS             5318..6298
                     /locus_tag="POB34_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI5919000.1"
                     /translation="MAIKKLDDGRYEVDIRPRGRDGKRIRRKFERKAEALAFERYTIA
                     NASQKEWGGQRADRRTLSELLDIWWKYHGQNHEHGTKEFNHLLKTINGIGDIPVSRMS
                     KRALMDYRSMRLRDGISAATINRDMYRLSGMFTKLIQLDEFSGQHPIHGLPPLAEANP
                     EMTFLEKAEIEKLLNVLAGDDLLVALLCLSTGGRWTEVATLKPAQITNCRVTFLKTKN
                     GKKRTVPISEELEKKVKEEASAKLFKVDYEKFCGILRRVKPDIPPNQATHILRHTFAS
                     HFMMNGGNIIALQQILGHASIQQTMAYAHLAPDYLQNAVALNPLKGGVTL"
     gene            complement(6485..6985)
                     /gene="cpxP"
                     /locus_tag="POB34_002053"
     CDS             complement(6485..6985)
                     /gene="cpxP"
                     /locus_tag="POB34_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HDI5919001.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            7135..7833
                     /gene="cpxR"
                     /locus_tag="POB34_002054"
     CDS             7135..7833
                     /gene="cpxR"
                     /locus_tag="POB34_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HDI5919002.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            7830..9203
                     /gene="cpxA"
                     /locus_tag="POB34_002055"
     CDS             7830..9203
                     /gene="cpxA"
                     /locus_tag="POB34_002055"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HDI5919003.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(9309..9983)
                     /gene="yiiM"
                     /locus_tag="POB34_002056"
     CDS             complement(9309..9983)
                     /gene="yiiM"
                     /locus_tag="POB34_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HDI5919004.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWKEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(10132..11115)
                     /gene="kdgT"
                     /locus_tag="POB34_002057"
     CDS             complement(10132..11115)
                     /gene="kdgT"
                     /locus_tag="POB34_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HDI5919005.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTTEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(11375..11995)
                     /gene="sodA"
                     /locus_tag="POB34_002058"
     CDS             complement(11375..11995)
                     /gene="sodA"
                     /locus_tag="POB34_002058"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HDI5919006.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            12280..13314
                     /gene="rhaT"
                     /locus_tag="POB34_002059"
     CDS             12280..13314
                     /gene="rhaT"
                     /locus_tag="POB34_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HDI5919007.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(13311..14177)
                     /gene="rhaR"
                     /locus_tag="POB34_002060"
     CDS             complement(13311..14177)
                     /gene="rhaR"
                     /locus_tag="POB34_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HDI5919008.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(14233..15069)
                     /gene="rhaS"
                     /locus_tag="POB34_002061"
     CDS             complement(14233..15069)
                     /gene="rhaS"
                     /locus_tag="POB34_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HDI5919009.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVAEQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            15357..16826
                     /gene="rhaB"
                     /locus_tag="POB34_002062"
     CDS             15357..16826
                     /gene="rhaB"
                     /locus_tag="POB34_002062"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDI5919010.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLKERKINDLPALIVATQSLPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVARIHSTRKTKELCA"
     gene            16823..18082
                     /gene="rhaA"
                     /locus_tag="POB34_002063"
     CDS             16823..18082
                     /gene="rhaA"
                     /locus_tag="POB34_002063"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDI5919011.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            18165..18989
                     /gene="rhaD"
                     /locus_tag="POB34_002064"
     CDS             18165..18989
                     /gene="rhaD"
                     /locus_tag="POB34_002064"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDI5919012.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWSFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            18999..19313
                     /gene="rhaM"
                     /locus_tag="POB34_002065"
     CDS             18999..19313
                     /gene="rhaM"
                     /locus_tag="POB34_002065"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDI5919013.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            19614..20060
                     /locus_tag="POB34_002066"
     CDS             19614..20060
                     /locus_tag="POB34_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDI5919014.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFIADSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            20071..21522
                     /locus_tag="POB34_002067"
     CDS             20071..21522
                     /locus_tag="POB34_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HDI5919015.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTLVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            21512..22582
                     /locus_tag="POB34_002068"
     CDS             21512..22582
                     /locus_tag="POB34_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDI5919016.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGDKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPSVIGSVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAAFKNCATQ
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            22582..24330
                     /locus_tag="POB34_002069"
     CDS             22582..24330
                     /locus_tag="POB34_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HDI5919017.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGYNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLNCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLEDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAHPIEVNNEIINHVLIACAAADARHELKVFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(24380..25420)
                     /locus_tag="POB34_002070"
     CDS             complement(24380..25420)
                     /locus_tag="POB34_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HDI5919018.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFK
                     QGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEAVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKTFHYYSLAVMISAKQINNLISQDKFDAEAAMKKVSELETLVAQAKEADK
                     GGMNFSFINSAGQYQLEAKKYVRRVRDKVPYSDWDKEQLQDANSSWMVDDSFPRALRE
                     YNEMVDDYNSLR"
     gene            complement(25573..26406)
                     /gene="fdhD"
                     /locus_tag="POB34_002071"
     CDS             complement(25573..26406)
                     /gene="fdhD"
                     /locus_tag="POB34_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDI5919019.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         6580 a         6871 c         6632 g         6564 t
ORIGIN      
        1 tggtgatatg aatatgacgc gcgccgagat gcggcgcgta aatgccgacc actctctcga
       61 cttcttcctc gtactcgtta acttcatccg acgggctacg ggcaaccctg acagtctgac
      121 aatcgcgcgg gacatttgcc ccaccctgcg cgctgatata caacgcaaaa tcaccactgt
      181 ctgcggcggc gcgtgcagcc tcgacgcgct cgtcaaactc atcagcaatg ctgacgccgc
      241 taggcaattt gcgtagttca cggtaagccc ccattgtcgg caggccaacc gttttaaatt
      301 gtgggatacg ccacgttgac gcccatgcgg taacagccgc tgcagtatct ttcagcggcc
      361 tgccggtatc gttatcgagc tgaccatcca gtgcatagcc gtcgatgttt tttgaaatgt
      421 atttcgcgat ataccccgca gcaccgcccc ggttaagatg ttttgcctga aaacggtttc
      481 gcgcagctcc tctttcgtcg ccatcctctt tgagcgcgta gcgacgcatg atttcaataa
      541 tctggttacg ctggcgtgga ttacaaaaaa gcatcatatg ccagtgcggc gttccgtcgt
      601 ggtgtggctc gacgactcgc aaaccgtaga cctgtaaatc attatccttg aatgccgtgc
      661 gcatcaggct ccagatacgg cagagatatc gctgcgcatc ctttggatta aatgcctcat
      721 cattccagcc gtgattaagc tggacggttt tactttcgcc ttttccgacc tgacgtgtcg
      781 ggtgatactt tgacggcgcg gtcagcgtga taaacatccc cacatcgccc tctgcggcgg
      841 cgtaacgctc aataccggca atggtgttca tcagctccat ccggcgaatt tcaggattag
      901 aaatactgcc catcacctta ctgataaggt cgatgcgctc gccggtttcc ctgttttcaa
      961 ggtcacacga tttaagaaat tccagatttg cctggcggcg cgcacgcaca tcacgaatgg
     1021 catgtttact ggcataagga gaacggtctt tattcacctc cccgacagca atcagtaacg
     1081 cctcatgcca gcgcatacgc tggcctttaa gctgactaat ccaccactca tcgttaaaca
     1141 ggcgggcaat ggcagaatat gcctgcctcg tggtcatctg tcctttacgg tattttttcc
     1201 agaaaagcgg ggaaatattg aaagcacgtg cagcgccagc aacatgacca tacagatgcg
     1261 cctgcgcctc atctgtaaac agcgattctt tctcgccatg tgcatcaacc caggcatcgc
     1321 agagttcctc atacatcata aaaagctgcg atgaaatacg ggcggcaaac tttttcagct
     1381 ccttgtcatt catccccggc agacgcgcat aatggtcacg ctctgccaga aacagcaacg
     1441 acgcgtcggt gttcatttca tggcgctgat tcacgcgctc aatacgcggc cataaacggc
     1501 gctgaaaagt ggatgtgagg aaataaaacc cgtgtagcgg gcttttattg cgccgtatgt
     1561 agtcataacg tgaagtaaac agcgagcgca aaaagtaagg caggcggtta atcgtggata
     1621 aaacaccttg cacctgacgc atctcgtcac gtgtaagggg tctttcgcgc ccgacagcct
     1681 cgcgtggcgt attccatgca taagcaccgg taaacgcctt accggtgcct gcggcaaacg
     1741 cagacggagg tacgcaggtg tattgctcct ggaaagaatg actcattcac atacaccggc
     1801 atagacacta ctgcaaacgg ctttatcatt cgccgaagcc agtaaatcaa actgagtacc
     1861 cccacgcgtt gtcattgtcc agtcatggta ggtttcaatt ccgtatgcct cgactgttac
     1921 aacctcaata cgacgttctg cgcgtagcgg atcatgtgtg gatgggaaaa aagtggaatt
     1981 tccccgacgg gagcacgacg caaccagtcg ttcccattca gcaacccgct ttatttcctc
     2041 cggccaccgg cgaaaaattt ctgccagttc ggatttacgg acatgaatac atggcataca
     2101 accaacacgg ccacacccct gctgatataa cggatttggc ttaatgtcat gtcttctggc
     2161 tattgcaaat acatcttcat gcttccagtg caaaatcggg cgatatacat gcaatccggg
     2221 ggtgttgtct gcatcctctt cccagacagg taaagctgcg cgtgatggcg attcctgagc
     2281 acgcactccc tgccagctaa tcacctcctc aaattcattg agtaaaggca ggattacctg
     2341 agaacgcacg ggttcatgtt tcagttcaaa tgtgcaaaat ctggctttgg ttgatgggaa
     2401 acgccccttc cacagacaca aatcaagaaa cgggataccc gttggtttga gaatttccag
     2461 cgcacaactg atgcgctggt gcgcttcttc ttccgacatt ccacaatcgt taaccaacaa
     2521 atcaggccat ttggtctgga taaacttgcg ctttgattca atccggcagg aaaaatcagc
     2581 tctgactctt ttaattgggc caagccgttt ttccagataa tccagatatt tcattgtctg
     2641 tggatgttca tgtcccgtat cagcaaaaac cggcaatatg ggtacatttt cttcaatagc
     2701 gacaagccac tgagcaaggc tgtcctttcc acctgaaaca aaaataacat tgatggtatt
     2761 ttcagtatgg caacgagcat cagtaatcat tcagacgctc ctctggagaa cgcctctgaa
     2821 caacgcttgc tgagctgctc aacctgcgtg tttaaatcgg caaaagattt tgcgcttccg
     2881 gtcagaatat cgtgatgcat caggccggaa acgagctggc ttaatttcgg ataataacca
     2941 accaccgcca gccattcctg accggcgttt ttaccgcttt ccgctctctt tttctcgtgg
     3001 agaataaact gaaagctgtc actggtaacg acataacgtt cgccaatttc aatacgaata
     3061 ctcatgccgt tctccggtaa tgcttgtttt ttgcttcaaa gactgactgg caggaaacac
     3121 aacgcgtggc tgacggataa gccgcacgac gggcagcagg tattggcgcg tcacactctt
     3181 cgcaaaccag cgcagaaaca ccgcaatgtt ttacccttgc cgcgttaatc tggcgctcca
     3241 gtaattcagc ctgttgttcc tgaataaaat ctacgttgtc cggcattacc agctcctttt
     3301 gtcgttcagc ttcttaaatt catcagcgca ataactggcg atttctgtcg ttaatttcgt
     3361 cagttcatcc actgaggaaa tttgcttgtg gaatacagcg cgtttaacaa gtaaattgac
     3421 cacatcagac aggaggttta attcattctg ataaatcgcg ataacagatt cagtgatgtc
     3481 gcgtttttct ttatcaagac aaagttgaat aagagacaaa tcaccatttt ccataacggc
     3541 gatttttaag gcgttattca gtaatacaac tgaatgagaa caggacatca aagtacctcc
     3601 ccgcgagaca atccgatatt gtgaaatttt tccgactcct gactgagcag ctcgactatc
     3661 tccacgcggg ataactccgc ctttgtgatg tggcgaatca tggcgtcaag atgagaagaa
     3721 aagcgcgtcg ccgcgtcggc ctgtgcttcg gttctggcct gttgcagcag taatgcgtat
     3781 ttaccgcact gattttcaga aactgtatgc atgactttct ccaggcaaaa agaagccccg
     3841 cacaattaag tgcgttaaaa actctggtta attacttaat gcagatattg ctctggtttt
     3901 accgacgtca gaattgtcgg tgcatactca aacaggctga ataattcacg taatgcacgg
     3961 aataaagcat cacgccagta acatgactct tcattaattc gccagtatgg ctggttgaat
     4021 tctttttcag tcaatccggc atgcataaat aaagtacgac gctgactgac tgttaaaaaa
     4081 ctaatatatg catactcact tgcaccgacc tgacggcgtt ttgagaatgc cccacgcaat
     4141 tcatcaattg cacaaaccag ccgttcacgt tcgacgtcgt tcatttcttc aaaacgcatc
     4201 gttgcgtgac gttgttttaa ctgcgcatga aagcaaactg ttagccgttc gcgctccatc
     4261 atctgattat aataatcaca tgtatcctgc cagcgaggaa cggcaagatg cttaccaatt
     4321 atccggcgca tggttgctgg ctgtttttca acgagattga gcgtcatcac tgtcatttcc
     4381 agaccctccg gcttttcaga aaggtcagag ccttttttaa cggactctgt tttttggtgc
     4441 ggataatgat tcccttacgc cccttaccgt gggtgatggt gaagtcaatc gccctggggc
     4501 tttcgttacg cagtaactga gcaatacaac gcggctcatt cataatcaca accccatcca
     4561 caaaagccat gcatcacgct gttcaaccgg tcggttataa aacgcctcac gtacagcgcg
     4621 attaaactca ggaatgaaaa cccatttttc accggcacga gccttcggtt tgcaaggatc
     4681 acgcaattca ataattggta atttatttgc cttcaccatt tcactgacgg ctgtctttgg
     4741 cttccctaat aaatcagcaa atttatccac atgaaccgca tcaagcggat actgaatcac
     4801 ataattttca gcgtccatat atggtaccct cataggatcc agccctttct aaaccactca
     4861 aaaccgttta gacgctggtt tattctcaaa tcaatggaac ctatataggt tccagttttg
     4921 agggaattta gtccctatat aggcaccatg tcaaatgaaa ttaagcgaaa agattaaggc
     4981 cttgcgtgag gctgaagggc taagccaatc aaaattctgt gaaatcatag agttaccgct
     5041 aagcacactt aaaaaatatg aaggaggaaa ctttgaaccc ggtggcacag ctttgctaaa
     5101 aatcactatg catcccacat tccaaaaata tgctctatgg cttatgacag ataaaaccgc
     5161 gccggacgca ggacaaatcg caccggctct cgcgcacatt gggccagagt caacagagtc
     5221 caaccactcc gcgaaaagga ttggctaact ctatataaag attacatttt caccatttgc
     5281 taccaagatg gtgaatacag cgccggaggg ctttcttatg gcaattaaga agctcgatga
     5341 cggtcgctat gaagtggaca ttagacctcg cggtcgcgac ggaaaacgca tccgcaggaa
     5401 attcgaaaga aaagctgaag cactagcatt tgagcgatac acaatcgcca atgccagtca
     5461 gaaagaatgg ggaggccagc gagcagaccg ccgaactttg agtgagttgc tggacatctg
     5521 gtggaaatat cacgggcaaa accacgagca tggaacaaaa gagtttaatc atctactcaa
     5581 aaccatcaac ggcataggtg atataccagt gagccggatg agcaaaaggg ctttgatgga
     5641 ttatcgttcc atgcgactac gtgatggtat cagtgccgca acgataaacc gtgacatgta
     5701 ccgattatcc ggcatgttca caaaattaat tcaattggat gaattttccg ggcaacaccc
     5761 aattcacgga ctgccgccac tggcggaggc caaccctgaa atgacgttcc tggaaaaagc
     5821 agaaatcgaa aaactgttaa atgttttggc tggtgatgac ttacttgtcg cgcttttatg
     5881 tctgagcact ggaggaagat ggacggaagt tgccacgcta aaaccagcac agattacaaa
     5941 ttgcagggtt accttcctga aaaccaaaaa cggtaaaaag cgaaccgtgc cgatttctga
     6001 ggaactggag aaaaaagtta aagaggaggc cagcgccaaa ttattcaaag ttgattatga
     6061 gaaattttgc gggattttac gcagagtgaa acctgatata ccacccaatc aggcaaccca
     6121 catcctgcgg catacattcg caagccattt catgatgaat gggggcaata taatcgcact
     6181 gcaacagatt ctgggacatg cgagcattca gcagacgatg gcctatgcgc accttgcgcc
     6241 tgactacctg caaaatgccg tcgctctgaa tccactaaaa ggcggagtga cgttataaat
     6301 ttccccctct gagtgtccac atagtgtcca cactcaagaa attttgaaac cactccaaac
     6361 ccttacagct ttcgataagt gactgttttt acttttgatt gcatgtaagt aattgataaa
     6421 aaaaaccccc acatcatgtg ggggaagaca gggatggtgt ctatggcaag gaaaacaggg
     6481 tttactactg ggaacgtgag ttgctactac tcaatagctt caacgatgaa cttttttgcc
     6541 attgcgtcac gtcgcgcaac tgctccattc gttgttgatg tttctcgttt aaaaccgctt
     6601 gctgctccgg cgttaacagg cgatacattt ggttgcggac ttttgccatc tcaacctgac
     6661 gagcaatttg ctcattcgcc attttttctg cctgtgcgcg cacagcgttt tcatcaaaat
     6721 tttctgcggt gacaaggcga tgcattgtct ccagttcgct aacattaaca ggaggctgtt
     6781 cgtgccgggc ctgttgcata agatctcgca tctgctgacg ctgatgttcg gttaaactta
     6841 tgccgtcgaa catatggctc tgcgtactgc gctgcgtaag ttcttcaccc ggatgccagt
     6901 tatcgcctga accgacttca gcagcgtggc ttaatgaact gactgccagc gttgaggcca
     6961 tgacggcagc ggtaactatg cgcatcattt gctcccaaaa tctttctgtc gcgattcaac
     7021 gatagagagt ttacgattca ggctgcaaac atgcgtcagg gggtgtaaaa caacgtaaag
     7081 tcatggatta gcgacgtctg atgacgtaat ttctgcctcg gaggtattta aacaatgaat
     7141 aaaatcctgt tagttgatga tgaccgagag ctgacttccc tattaaagga gctgctcgag
     7201 atggaaggct tcaacgtgat tgttgcccac gatggggaac aggcgcttga tcttctggac
     7261 gacagcattg atttactttt gcttgatgta atgatgccga agaaaaatgg tatcgacaca
     7321 ttaaaagcac ttcgccagac acaccagacg cctgtcatta tgttgacggc gcgcggcagc
     7381 gaacttgatc gcgttctcgg ccttgagctg ggcgcagatg actatctccc gaaaccgttt
     7441 aatgatcgtg agctggtggc acgtattcgc gcgatcctgc gccgttcgca ctggagcgag
     7501 caacagcaaa acaacgacaa cggttcaccg acactggaag ttgatgcctt agtgctgaat
     7561 ccaggccgtc aggaagccag cttcgacggg caaacgctgg agttaaccgg cactgagttt
     7621 accctgctct atttgctggc acagcatctg ggtcaggtgg tttcccgtga acatttaagc
     7681 caggaagtgc tgggcaaacg cctgacgcct tttgaccgcg ctatcgatat gcacatttcc
     7741 aacctgcgtc gtaaactgcc ggatcgtaaa gatggtcacc cgtggtttaa aaccttgcgt
     7801 ggtcgcggct atctgatggt ttctgcttca tgataggcag cttaaccgcg cgcatcttcg
     7861 ccatcttctg gctgacgctg gcgctggtgt tgatgttggt tttgatgtta cccaagctcg
     7921 attcacgcca gatgaccgag cttctggata gcgaacagcg tcaggggctg atgattgagc
     7981 agcatgttga agcggaactg gcgaacgatc cgcccaacga tttaatgtgg tggcggcgtc
     8041 tgtttcgggc gattgataag tgggcaccgc caggacagcg tttgttattg gtgaccaccg
     8101 aaggccgcgt gatcggcgct gaacgcagcg aaatgcagat cattcgtaac tttattggtc
     8161 aggccgataa cgccgatcat ccgcagaaga aaaagtatgg ccgcgtggaa ctggtcggtc
     8221 cgttctccgt gcgtgatggc gaagataatt accaacttta tctgattcgt ccggccagca
     8281 gttctcaatc cgatttcatt aacttactgt ttgaccgccc gttattactg ctgattgtca
     8341 ccatgttggt cagtacgccg ctgctgttgt ggttggcctg gagtctggca aaaccggcgc
     8401 gtaagctgaa aaacgctgcc gatgaagttg cccagggaaa cttacgccag cacccggaac
     8461 tggaagcggg gccacaggaa ttccttgccg caggtgccag ttttaaccag atggtcaccg
     8521 cgctggagcg tatgatgacc tcccagcagc gtctgctttc tgatatctct cacgagctgc
     8581 gcaccccact gacgcgtctg caactgggta cggcgttact gcgccgtcgt agcggtgaaa
     8641 gcaaggaact ggagcgtatt gaaaccgaag cgcaacgtct ggacagcatg atcaacgatc
     8701 tgttggtgat gtcacgtaat cagcaaaaaa acgcgctggt tagcgaaacc atcaaagcca
     8761 accagttgtg gagtgaagtg ctggataacg cggcgttcga agccgagcaa atgggcaagt
     8821 cgttgacggt taacttcccg cctgggccgt ggccgctgta cggcaaccca aacgccctgg
     8881 aaagtgcgct ggaaaacatt gttcgtaatg ctctgcgtta ttcccatacg aagattgaag
     8941 tgggctttgc ggtagataaa gacggtatca ccattacggt ggacgacgat ggtcctggcg
     9001 ttagcccgga agatcgcgaa cagattttcc gtccgttcta tcggaccgat gaagcacgcg
     9061 atcgtgaatc tggcggtaca ggtttggggc tggcgattgt tgaaaccgcc attcagcagc
     9121 atcgtggctg ggtgaaggca gaagacagcc cgctgggcgg tttacggctg gtgatttggt
     9181 tgccgctgta taagcggagt taaactccgc atttgtaggc aggataaggc gtttacgccg
     9241 catccggcat ttgagcagga tgcctgatgc gacgctgata gcgccttatc aggcctacac
     9301 tccgggggtt attttcccca caaccggcgg gaaaaatctt cgatcttgcc gctcagtcgg
     9361 cgcttttgca tcgtccgcgt ccagcttttc gataacccgg cggcggagag taaacggtga
     9421 tattgatcgt catcaaacgg catatgccat gcgatggcgg cagcctcctg cacggtcaca
     9481 tcgctgacac gggaaaccaa ctccagcggc gcgtccgcag acactttccc tggcgctatc
     9541 acgctataca accaccccac tttacccgtg ttttgcatca actgcgctat atcgctgata
     9601 tcaaaatggt aattgagttt atagcaaggc gagcgcggct ggctgacctg aattaatgcc
     9661 tctttccagc ggaaaatatc gcccatataa acattacttt ccgtgagacc gtcggttgag
     9721 agattttcgc caaacgcagg cgcaacaaac aactccgcct gttccggaaa ttcccgcgcc
     9781 cagtagagat agtgctcacg aggataatga cacagcgctc tgtctggccc gccgtgaacc
     9841 tttttctccg cctgctcgtc accttccagc cccagctccg tcagcatcaa ctcaccatca
     9901 acctggattt tggcaattgc gctgggtttg ccttcgggat aagcctgaat cttgcctgtg
     9961 tatacatcaa ccggatatcg catctgttcc ctctccacga gaaatttccc cattagacca
    10021 tatttatttt atgtcaggcg tgctgttttc gcggtaagta tccaggcagg aactcaatag
    10081 caaaccggaa gaacgttagt tcatccggct gtggagatta agaaagcagg tttatttcac
    10141 cgtacctaaa atttcgattt tcgctgctct ggctttgact ttacgtgacc agatagaggt
    10201 aagaattggc accagaatcg aagtcacaat gaccgccgtc gctaccagcg aagttgctgc
    10261 cggagccatc ggtttaaacg caggcaccat ttctgcaatc agcacaggtg tcgctaccgc
    10321 ggcccctgcg gaactggaag cggcaattcc ggcagtgcca tcgccaccgc caatcaattt
    10381 atcggcgata atcaacggaa taccggtcac gataattact gccacaccca acaggatccc
    10441 cagcaaacca gtctgggcaa tcacagtcaa atcaatggta ttgcccagcg cgaaggcaaa
    10501 gaatggaatc agcgtttgca ccgctttgct gaaaaattct cgtaattcag ggtcaaggtt
    10561 ccccagggca aagcccacca ggaacggtaa tactgcgccg acgaaaacat gcggttcaaa
    10621 cgaggcaatc cctgcagtgc ccagaataat catcgtcatg agcggcccgg actccaacga
    10681 catcaacaca aatgccccag cttcttctgt tgtgccgtac tgctgcatga tggaagcgta
    10741 aagtccgcca ttggtcatat ccatcgccgc caccagcgcc agcgttgaaa gtccggcaaa
    10801 gaatccaact tcaacaccat gttccggaat aatgcgcgag gcaattgccg caaccaccca
    10861 cgcgacggca atttttgtta ccaccagcgt accggattta cgcaataccg ttcccgtcgc
    10921 gcttaatttt attgacgccc ccatgcaaaa aaaccacacc gccaaaatgg gcaccgtacc
    10981 ggtaatcatc ccgttggtga atgatccaaa atatttcccc gcccccggcg agaaggtgtg
    11041 gcacagtgcg ccaaggaata gcgggacgag catcatcccc cccgggattt tctcaatcga
    11101 gcgttttatc tgcatttcca tcacctgtcg gcatattttt aagggctgat gcgtcattcc
    11161 tcaatggagc atcagtgttg atggaaacga agatagacat tcatttaatc aataaaagtg
    11221 atcaaaacca catcaattga aacactgttt tatttttatc ggatcattgt ttttattttg
    11281 aaattgatca caaaaaaacc accgccgttg gcgatggttc attttttaag ctgatatgcg
    11341 gcctatacgc ctcattgcag caggcggcaa atgattattt tttcgccgca aaacgtgccg
    11401 ctgcttcgtc ccagttcacc acgttccaga actctttaat gtagtccgga cggcggttct
    11461 ggaatttcag gtagtaagca tgttcccaca catccaggcc cagaatcggg aagccggaag
    11521 cgccagaaat agcttcaccc atcagcggag aatcctggtt agcagtagaa accaccgcca
    11581 gtttatcgcc tttcagcacc agccatgccc agccggaacc aaagcgggaa gctgccgctt
    11641 tttcaaattc tgctttgaag ttatcaacgg agccgaagtc acgttcgata gccgctttca
    11701 ggtcaccctg cagggtggtg ccttttttca gacctttcca gaacaggctg tggttagcgt
    11761 gaccgccagc gttgttgcgc agtacggttt tcttgtctgc tggcagctgg tccagtttgg
    11821 tgatcagctc ttcaaccggc aggttggcaa attctggcag gctttccagc gccgcgttgg
    11881 cgttgtttac gtaggtctga tggtgtttgg tgtggtggat ttccatggtc tgcttatcga
    11941 agtgcggttc cagggcatcg taagcatacg gcagggatgg cagggtatag ctcatattca
    12001 tctccagtat tgtcgggcgg ccgattgtta atgccgcgta agcagttggt tcattatagt
    12061 taattaaatg atattgaaaa tgattatcaa tgccgtactt ttcgtaaggg tatggttttg
    12121 caggaaaatg cccgagatgt gaagcaaatc acccacttaa tgccgtgatt gccagtaaat
    12181 cgacaacggc ggcaacaggc gaaaggttaa tcgacagcac gatttttaca ctcatctcgt
    12241 cggagatgtg aagcgacgaa aaatgatgag gataagaaga tgagtaacgc gattacgatg
    12301 gggatatttt ggcatttgat cggcgcggcc agtgcagcct gtttttacgc tccgttcaaa
    12361 aaagtaaaaa aatggtcatg ggaaaccatg tggtcagtcg gtgggattgt ttcgtggatt
    12421 attctgccgt gggccatcag cgccctgtta ctaccggatt tctgggcgta ttacagctcg
    12481 tttagtctct ctacgctgct gcctgttttt ctgttcggcg ctatgtgggg gatcggtaat
    12541 atcaactacg gcctgaccat gcgttatctc ggcatgtcga tgggaattgg catcgccatt
    12601 ggcattacgt tgattgtcgg tacgctgatg acgccaatta tcaacggcaa tttcgatgtg
    12661 ctgattaaca ccgaaggcgg acgcatgacg ttgctcggcg ttctggtggc gctgattggc
    12721 gtagggattg taactcgagc cgggcagttg aaagagcgca agatgggcat taaagccgaa
    12781 gagttcaatc tgaaaaaagg gctggtgctg gcggtgatgt gcggcatttt ctctgccggt
    12841 atgtcttttg cgatgaacgc cgcaaaaccg atgcatgaag ccgctgccgc acttggcgtc
    12901 gatccactgt atgtcgctct gccaagctat gtcatcatca tgggcggcgg cgcgatcatc
    12961 aacctcggtt tctgcttcat tcgtctggca aaagtgaagg atttgtcgct aaaagccgac
    13021 ttctcgctgg caaaaccgct gatcattcac aatgtgttac tctcagcact gggcgggttg
    13081 atgtggtatc tgcaattctt tttctatgcc tggggccacg cccgcattcc ggcgcagtat
    13141 gactacatca gttggatgct gcatatgagt ttctatgtat tgtgcggcgg tatcgtcggg
    13201 ctggtgctga aagagtggaa caatgcaggt cgccgtccgg taacggtgtt gagcctcggt
    13261 tgtgtggtga ttattgtcgc cgccaacatc gtcggcatcg gcatggcgaa ttaatctttc
    13321 tgcgaattga gatgacgcca ctggctgggc gtcatcccgg tttcccgggt aaacaccacc
    13381 gaaaaatagt tactatcttc aaagccacat tcggtcgaaa tatcactgat taacaggcgg
    13441 ctatgctgga gaagatattg cgcatgacac actctgacct gccgcagata ttgattgatg
    13501 gtcattccag tctgctggcg aaattgctga cgcaaaacgc gctcactgca cgatgcctca
    13561 tcacaaaatt tatccagcgc aaagggactt ttcaggctag ccgccagccg ggtaatcagc
    13621 ttatccagca acgtttcgct ggatgttggc ggcaacgaat cactggtgta acgatggcga
    13681 ttcagcaaca tcaccaactg cccgaacagc aactcagcca tttcgttagc aaacgacaca
    13741 tgctgactac tttcatgctc aagctgaccg ataacctgcc gcgcctgcgc catccccacg
    13801 ctacctaagc gccagtgtgg ttgccctgcg ctggcgctaa atcccggaat cgctccctgc
    13861 cagtcaagat tcagcttcag ccgctccggg caataaataa tattctgcaa aaccagatcg
    13921 ttaacggaag cgtaggagtg tttatcgtca gcatgaatgt aaaagagatc gccacgggta
    13981 atgcgataag ggcgatcgtt gagtacatgc agaccattac cgcgccagac aatcaccagc
    14041 tcacaaaaat catgtgtatg ttcggcaaaa acatcttgcg gataacggtc agccacagcg
    14101 actgcctgct ggtcgctggc aaaaaaatca tctttgagaa gttttaactg atgcgccacc
    14161 gtggctacct cggccagaga acgaagttga ttattcgcaa tatggcgtac aaatacgttg
    14221 agaagattcg cgttattgca gaaaaccatc ccgtccctga cgaatatcac gcggtgacca
    14281 gttaaactct cggcgaaaaa gcgtcgaaaa gtggttactg tcgctgaatc cacagcgata
    14341 ggcgatatca gtaacgctgg cctcgctgtg gcgtagtaga tgtcgggctt tcatcagtcg
    14401 caggcggttc aggtaacgct gaggcgtcag tcccgtttgc tgcttaagct gccgatgtag
    14461 cgtacgcagc gaaagagaaa attgctccgc cacggcatcc caattcacct catcggcaaa
    14521 atggtcctcc agccaggcca gaagcaagtt gagacgtgat gcgctgtttt ccaggttctc
    14581 ctgcaaactg cttttacgca gcaagagcag taattgcata aacaagatct cgcgactggc
    14641 ggtcgagggt aaatcatttt ccccttcctg ctgttccatc tgtgcaacca gctgtcgcac
    14701 ctgctgcaat acgctgtggt taacgcgcca gtgggaagga tactgcccat ccagctcttg
    14761 tggcagcaac tgattcagcc cggcgagaaa ctgaaatcga tccggcgagc gatacagcac
    14821 attagtcaga cacagattat cggtatgttc atacagatgc cgatcatggt cgcgcacaaa
    14881 acagaccgtg ccaccggtga tggtataggg ctgcccatta aacacatgaa tacccgtgcc
    14941 atgttcgaca atcacaattt catgaaaatc atgatgatgt tcaggaaaat ccgcctgcgg
    15001 gagccggggt tctatcgcca cggacgcgtt accagacgga aaaaaatcca cactatgtaa
    15061 tacggtcata ctggcctcct gatgtcgtca acacggcgaa atagtaatca cgaggtcagg
    15121 ttcttacctt aaattttcga cggaaaacca cgtaaaaaac gtcgattttt caagatacag
    15181 cgtgaatttt caggaaatgc ggtgagcatc acatcaccac aattcagcaa attgtgaaca
    15241 tcatcacgtt catctttccc tggttgccaa tggcccattt tcctgtcagt aacgagaagg
    15301 tcgcgaattc aggcgctttt tagactggtc gtaatgaaat tcagcaggat cacattatga
    15361 cctttcgcaa ttgtgtcgcc gtcgatctcg gcgcatccag tgggcgcgtg atgctggcgc
    15421 gttacgagcg tgaatgccgc agcctgacgc tgcgcgaaat ccatcgtttt aacaatgggc
    15481 tgcatagtca gaacggctat gtcacctggg atgtggatag cctggaaagt gccattcgcc
    15541 ttggattaaa caaggtgtgc gaggaaggga ttcgtatcga tagcattggg attgatacct
    15601 ggggcgtgga ctttgtgctg ctcgaccaac agggtcagcg tgtgggcctg cccgttgctt
    15661 atcgcgatag ccgcaccaat ggcctgatgg cgcaggcaca acaacaactc ggcaaacgcg
    15721 atatttatca acgtagcggc atccagtttc tgcccttcaa tacgctttat cagttacggg
    15781 cgctgacgga gcaacaacct gaacttattc cacacattgc tcacgctctg ctaatgccgg
    15841 attattttag ttatcgcctt accggcaaga tgaactggga gtacaccaac gccacaacca
    15901 cgcaactggt caatatcaat agcgacgact gggacgagtc gctactggcg tggagcggtg
    15961 ccaacaaagc ctggtttggt cgcccgacgc atccgggtaa tgtcataggt cactggattt
    16021 gcccgcaggg taatgagatt ccggtggtcg ccgttgccag ccatgatacc gccagcgcgg
    16081 ttatcgcctc gccgttaaac ggttcacgcg ccgcttatct ctcttctggt acctggtcat
    16141 tgatgggctt cgaaagccag acgccattta ccaatgacac ggcgctggca gccaacatca
    16201 ccaatgaagg cggggcggaa ggtcgctatc gggtgctgaa aaatattatg ggcttatggc
    16261 tgcttcagcg ggtgcttaag gagcggaaaa tcaacgatct cccggcgctt atcgtcgcga
    16321 cacagtcact tccggcctgc cgcttcatca tcaatcccaa tgacgatcgc tttattaatc
    16381 ctgaggcgat gtgcagcgaa attcaggctg cgtgtcggga aacggcgcaa ccgatcccgg
    16441 aaagtgatgc tgaactggcg cgctgcattt tcgacagtct ggcgctgctg tatgccgatg
    16501 tgttgcatga gctggcgcag ctacgcggtg aagatttctc gcaactgcat attgtcggcg
    16561 gaggctgtca gaacacgctg ctcaaccagc tatgtgccga tgcctgcggt attcgggtga
    16621 tcgccgggcc tgttgaagcc tcgacgctcg gcaatatcgg catccagtta atgacgctgg
    16681 atgaactcaa caatgtggat gatttccgtc aggtcgtcag caccaccgcg aatctgacca
    16741 cctttacccc taatcctgac agtgaaattg cgcactacgt ggcgcggatt cactctacac
    16801 gaaagacaaa ggagctttgc gcatgaccac tcaactggaa caggcctggg agctggcgaa
    16861 acagcgtttc gcggcggtgg ggattgatgt cgaggaggcg ctgcgccaac ttgatcgttt
    16921 acccgtttca atgcactgct ggcagggcga tgatgtttcc ggttttgaaa acccggaagg
    16981 ttcgctgacc ggggggattc aggccacagg caattatccg ggcaaagcgc gtaatgccag
    17041 tgagctacgt gccgatctgg aacaggctat gcggctgatt ccggggccga aacggcttaa
    17101 tttacatgcc atctatctgg aatcagatac gccagtctcg cgcgaccaga tcaaaccaga
    17161 gcacttcaaa aactgggttg aatgggcgaa agccaatcag ctcggtctgg attttaaccc
    17221 ctcctgcttt tcgcatccgc taagcgccga tggctttacg ctctcgcatc ccgacgacag
    17281 cattcgccag ttctggattg atcactgcaa agccagccgt cgcgtttcgg cctattttgg
    17341 cgagcaactc ggcacaccat cggtgatgaa catctggatc ccggatggta tgaaagatat
    17401 caccgttgac cgtctcgccc cgcgtcagcg tctgctggca gctctggatg aggtgatcag
    17461 cgagaagctg gatccggcgc accatatcga cgccgttgag agcaaattgt ttggcattgg
    17521 cgcagagagc tacacggttg gctccaatga gttttacatg gggtatgcca ccagccgcca
    17581 gactgcgcta tgcctggacg ccgggcactt ccacccgact gaagtgattt ccgacaagat
    17641 ttccgccgcc atgctgtatg tgccgcagtt gctgctgcac gtcagccgtc cggttcgctg
    17701 ggacagcgat cacgtagtgc tgctggatga tgaaacccag gcaattgcca gtgagattgt
    17761 gcgtcacgat ctgtttgacc gggtgcatat cggccttgac ttcttcgatg cctctatcaa
    17821 ccgcattgcc gcgtgggtca ttggtacacg caatatgaaa aaagccctgc tgcgtgcgtt
    17881 gctggaacct accgctgagc tgcgcaagct ggaagcggcg ggcgattaca ctgcgcgtct
    17941 ggcactgctg gaagagcaga aatcgttgcc gtggcaggcg gtctgggaaa tgtattgcca
    18001 acgtcacgat acgccagcag gtagcgaatg gctggagagc gtgcgggctt atgagaaagc
    18061 gattttgagc cagcgtgggt aaacactgcc ggatgcggcg cgagcgcctt atccggccta
    18121 cgggtcgtgc atctgacaac accgaattta caggaacaca gaacatgcaa aacattaccc
    18181 agtcctggtt tgtccaggga atgatcaaag ccaccaccga tgcctggctg aaaggctggg
    18241 acgagcgcaa cggcggcaac ctgacgctgc gcctggatga cgccgatatc gcgctttatc
    18301 acgacaactt ccacccacaa ccgcgctata ttccgctcag ccagcccatg cctttactgg
    18361 caaatacgcc gtttattgtc accggctccg gcaaattctt ccgtaacgtc cagcttgatc
    18421 ctgcagctaa cttaggcgtc gtaaaagtcg acagcgacgg cgcgggctac catattctct
    18481 gggggttaac caacgaagcc gttcccactt ccgaacttcc ggctcacttc ctttcccact
    18541 gcgagcgcat taaagccacc aacggtaaag atcgggtgat catgcactgc cacgccacca
    18601 acctgatcgc cctcacctat gtactggaaa acgacaccgc ggtctttact cgccaactgt
    18661 gggaaggcag caccgagtgt ctggtagtgt tcccggatgg cgttggcatt ttgccgtgga
    18721 tggtgcccgg cacggacgaa atcggccagg cgaccgcaca agagatgcaa aaacattcgc
    18781 tggtgttgtg gtccttccac ggcgtcttcg gcagcgggcc gacgctggat gaaacattcg
    18841 gtttaatcga caccgcagaa aaatcagcac aagtattagt gaaggtttat tcgatgggcg
    18901 gcatgaaaca gaccatcagc cgtgaagagt tgatagcgct cggcaagcgc ttcggcgtta
    18961 caccactagc cagtgcgctg gcgctgtaag gagcaaacat gatccgcaaa gcctttgtca
    19021 tgcaggttaa ccccgacgcc cacgaagagt atcagcgtcg gcataacccc atctggccgg
    19081 aactggaagc agtgttgaaa tctcacggtg cgcataacta cgccatttac ctcgacaaag
    19141 cgcgtaatct gctgtttgcc acggtagaga ttgaatctga agaacggtgg aatgcggtag
    19201 ccagtactga tgtttgccaa cgttggtgga aatatatgac cgatgttatg cccgctaacc
    19261 cggataacag cccggtgagt agcgagctgc aagaagtgtt ttacctgccg taattgccca
    19321 atgtgccgga gatgcctccg gcacaatatt ttaaatttcg atttgaaaaa ttaaacatat
    19381 tacatctccc ggtaagttat atttccctga tacattgtga gtaaatcaca aaaataatga
    19441 ataacccatt aatgattcat gtggtttatt taaataacca ttatgtgcat tactccgcaa
    19501 atctgacctt tcactctgtc tcaaattgtc attgtttacc gacatatcgc ctctcatcac
    19561 ttaatcgctc caccattaca attgattaaa atatatttta tggagtccga ttaatggcag
    19621 ctcttactgc aagctgtatt gacctgaata ttcagggcaa tggcgcttat tccgttctga
    19681 agcagttggc gacaatagcg ttacaaaacg gttttatcgc cgactcacac cagttcctgc
    19741 aaaccctgct gctgcgcgaa aaaatgcact ctactggatt tggttccggt gtcgccgtgc
    19801 cgcacggtaa aagcgcctgc gttaaacaac cgttcgtatt attcgcccgc aaagcgcagg
    19861 ctattgactg gaaagccagc gatggcgaag acgtcaattg ctggatctgc ctcggcgtgc
    19921 cgcaaagcgg cgaagaggat caggtcaaaa tcatcggcac actgtgtcgc aaaattatcc
    19981 accaggattt tattcatcaa ctgcaacagg gcgacgccga ccaggtgctt gccttgttaa
    20041 atcaaaccct cagctcataa ggaagtggcg atggagtcat ccttacgtat tgtcgcgatc
    20101 accaactgcc ccgccgggat cgctcacacc tacatggtgg cggaagccct ggaacagaaa
    20161 gcccgttctc tcggccatac cataaaagtg gaaactcaag ggtccagtgg cgttgaaaac
    20221 cgcttatcca gcgaagagat tgccgctgcc gattacgtca ttctcgctac cgggcgtggc
    20281 ctgagcggtg atgatcgcgc gcggtttgcc gggaagaaag tttatgagat tgccatctcc
    20341 caggcgttga aaaatatcga ccagattttc agcgaattac cgacaaactc gcagcttttt
    20401 gccgcagata gcggcgtgaa gcttggcaaa caggaagtgc aaagcggcag cgtgatgagc
    20461 cacctgatgg cgggcgtctc tgcggcgctg ccgtttgtca tcggcggtgg tatcctggtg
    20521 gcgcttgcca acatgctggt gcagttcggt ttgccgtata ccgatatgtc gaaaggcgcg
    20581 ccgtcgttta ccttggtagt tgaatccatc ggttaccttg gcttcacctt tatgatcccc
    20641 atcatgggcg cttatattgc ctcgtcgatt gccgataaac cggctttcgc tccggcgttt
    20701 ctggtttgct atctggcgaa cgacaaagcg ctgctcggca cccagtcggg tgcgggattc
    20761 ctcggcgcgg tggtgctggg gctggcgatt ggctatttcg tcttctggtt ccgcaaagtg
    20821 cgccttggca aagcattaca accgctgctc ggttcaatgc tgatcccgtt cgttactctg
    20881 ctggttttcg gcgtgctcac ttactacgtt atcgggccgg tgatgtccga cctcatgggc
    20941 gggctgctcc acttcctgaa taccattcct ccatcaatga agtttgcggc agcgtttctg
    21001 gtcggtgcga tgctggcgtt cgatatgggc ggccccatca ataaaaccgc ctggttcttc
    21061 tgcttctcac tgctggaaaa acacatttat gactggtacg ccatcgtcgg cgttgtcgcg
    21121 ttgatgccgc ccgttgcggc gggtcttgcg acgttcattg cgcccaaact gtttacccga
    21181 caggaaaaag aggctgccag tagtgccatt gtggtcggtg ccacagtcgc aaccgaaccg
    21241 gctatacctt acgctctggc agctccgttg ccgatgatta ccgccaatac gctggcgggt
    21301 ggcattaccg gcgtgctggt gatcgctttc ggaatcaaac gtctggcacc tggtttgggt
    21361 atctttgacc cgctgatcgg cctgatgtcg ccggtgggtt cgttttatct ggtgctggcg
    21421 attggcctcg cgctgaacat ttcattcatt atcgtcctga aaggattgtg gttacgccgc
    21481 aaagcgaaag ccgcgcaaca ggagcttgtc catgaacatt gagttactgc aacagttgtg
    21541 cgaagccagc gccgtcagcg gcgatgaaca ggaagttcgc gacattctga taaacacgct
    21601 ggaaccttgc gttaatgaga tcacctttga tggtctgggc agctttgttg cccgtaaggg
    21661 ggataaaggt ccaaaagttg ccgttgtcgg gcatatggat gaagtcggct ttatggtcac
    21721 ccacatcgac gagagcggtt ttctgcgttt caccaccatt ggtggctggt ggaatcagtc
    21781 gatgctcaac caccgggtaa ccatacgcac acacaaggga gtgaaaatcc ctagtgtgat
    21841 tggttccgtc gcgcctcatg cgttaacgga aaagcaaagg caacagccgc tgtcatttga
    21901 tgagatgttc attgatattg gcgcgaacag tcgcgatgag gtggaaaaac gcggtgtggc
    21961 gatgggcgat tttattagcc cggaagccaa ttttgcctgc tggggcgaag ataaagtggt
    22021 cggcaaggcg ctggataacc gcatcggctg cgcaatgatg gctgaactat tgcagacggt
    22081 gaataatccc gaaattacgc tgtatggcgt cggcagcgtg gaagaagaag ttgggctacg
    22141 cggggcacaa acctcggcgg aacacattaa accggatgtg gtgattgtgc tggataccgc
    22201 tgtcgcaggt gatgttccgg gcattgataa cattaaatac ccgctgaaac tgggccaggg
    22261 gccggggctg atgctgtttg acaagcgcta cttccccaac cagaaactgg tagcagcgtt
    22321 taaaaactgt gccacgcaga atgatttacc gctgcaattt tccaccatga aaaccggagc
    22381 gacggatggc gggcgctaca acgtgatggg cggcgggcgt ccggttgtcg cgctgtgtct
    22441 gccaactcgt tatctgcacg ccaacagcgg tatgatttca aaagccgatt acgatgctct
    22501 gctcacgctg atacggggtt ttctgacgac cttaactgcg gagaaagtca acgcgtttag
    22561 ccagttccgt caggtggatt aatgctaaac gaacgccagt taaagattgt cgatctgctg
    22621 gagcaacagc cgcgcacgcc tggcgagctg gcgcaacaga ctggcgtttc aggcaggacc
    22681 atcctgcgtg atattgacta tctcaacttc acccttaacg gcaaagcccg cattttcgcc
    22741 agtggcagtg cgggctatca gctggaaatc ttcgagcgcc gcagcttttt tcagttgctg
    22801 caaaagcacg ataacgacga tcggctgctg gcgctgttat tactgaatac tttcactccc
    22861 cgtgcgcaac tcgcctcggc gcttaatttg ccagaaacgt gggtagcaga gcgtctgccc
    22921 cggttaaaac agcgttatga acgcacttgt tgcctggcca gccgccctgg tttgggccat
    22981 ttcattgatg agacagaaga gaaacgcgtt atcttgctgg cgaacttgct gcgcaaagat
    23041 ccgtttttaa ttccgctggc gggcataaca cgagacaacc ttcagcattt atccacggcc
    23101 tgcgacaacc aacaccgctg gccgctcatg cagggtgatt atctctccag cctgattctg
    23161 gcgatttacg ccctgcgtaa tcaactgacc gatgagtggc cgcaatatcc cggtgacgag
    23221 ataaaacaaa tcgttgaaca tagcggtctg tttcttggtt ataacgctgt aagaaccctg
    23281 acgggtttga tagagaaaca gcatcagcaa gcgcaggtaa tttcagccga tcatgtgctg
    23341 gggttgctgc aaagggtgcc gggcatcgcg tcattgaata ttattgatac gcagctggtt
    23401 gagaacatta ccgggcattt attacgttgc ctcgccgcac cagtgtggat tgctgagcac
    23461 cgccaaagca gcatgaataa cctgaaagcc gcctggcctg cggcctttga tatgagtctg
    23521 cactttatta cgctactgcg tgaacagctc gatattcccc ttttcgacag cgatctgatc
    23581 ggtttgtatt ttgcctgtgc gctggagcgg catcaaaacg aacgccagcc gatcattttg
    23641 ctctcggacc agaacgcgat tgccactatt aatcagctcg ccattgagcg cgatgtttta
    23701 aattgtcggg taattattgc ccgtagctta agcgaacttg ttgccattcg cgaagagatt
    23761 gagccgttat tgatcattaa caatagccat tatttactgg aagacgcggt aaataattac
    23821 atcaccgtaa aaaatatcat tacggctgcc ggtatcgaac aaataaaaca ttttctggcg
    23881 acggcattta ttcgccagca accggagcgt tttttctctg cccccggaag ttttcattat
    23941 tcgaatgtac gcggtgaaag ctggcaacat attacccggc aaatttgtgc gcaattagtg
    24001 gcacaacacc atattaccgc cgatgaagca caacgcatca tcgcccgcga aggcgaaggt
    24061 gaaaacctga ttgttaatcg cctcgccatc ccacattgct ggagcgaaca ggagcgacgt
    24121 tttcgtggat tttttattac cctcgcccac ccgatcgagg tgaataacga aatcattaac
    24181 catgtgttga ttgcctgcgc cgccgccgat gcgcgtcatg agctaaaagt atttagctat
    24241 ctggcaagcg tattgtgtca gcatccggca gaggttattg ccgggttaac aggatatgag
    24301 gcatttatgg agttacttca caaggggtga actgtgtcgg atgcgccgca tccgacactg
    24361 catttatcat caaaaacgtt taacgcaggc tattatagtc atcaaccatt tcgttgtact
    24421 cgcgtaatgc tctcgggaaa gagtcgtcga ccatccagct tgagtttgcg tcctgcagtt
    24481 gctctttatc ccagtcagag tacgggactt tatctctgac gcggcgaacg tattttttag
    24541 cctcgagttg atactggcct gccgaattaa taaacgagaa attcatgccg cctttatccg
    24601 cttctttggc ctgcgccacc agcgtttcca gttcagacac tttcttcatt gccgcttctg
    24661 catcaaactt atcttgcgat atcaggttat taatttgttt cgctgaaatc atgactgcca
    24721 gagagtaata gtggaatgtt ttcccttctc tttcttcaat atttttcagt tccgcaagct
    24781 ggcgcttatc gttaatttcc tgaatcgccg catgataaga ttctgctaca gcttcaaagg
    24841 cttccagatt ttttaagaat gtctggtgca gcgttttccc tttcgcaaac gcatcgtctt
    24901 tataattttc ctgggtgtaa tatttatcca tttcgttaat ggtattaccc aacgccacgg
    24961 cagcatcgat ataactcacc gccacgccat caataggttg cagcgcaggc gttaacgcca
    25021 cagcactttt tacgcctttt tcacactcag cgaggttgga ttcactaatg ccgtagatgc
    25081 cataaacagt acgctcttca ccggtaggcc cctgtttaaa atctttcagc cagtcagcat
    25141 aacgcgccag actgctctgt acagggatct gtaacttgtt atagcacttg atatagacat
    25201 ccattttttc gtcgaaaacc gcttgttcat cgaccaccgg ttgcgcaggt gtttcaggtt
    25261 tagcttctgc ttttgcaacg ggagcttcag gagctggtgc ggcaggttga ctattggcag
    25321 gcgggggcgt ttctgtttcg gcttttttat catcacaacc cgtcagaccg agggccatga
    25381 tggcgactat aatagcggaa gataataaat tccttttcat ttaattgcct tataaatata
    25441 tagataagag agtgaacata ataaatgaaa aaagaaaacg ccactacacg cgttttattt
    25501 ataaataaaa taataaaatt cacgaaatga ataagtcctg caatttaata tatatcgcag
    25561 gacttatttt aattaattgc tcaaacgctg cggatgggta taaaccgttg cccgacccgg
    25621 cttacaaaaa cccaccagcg tcagattaca acgctcagcc acttctacag caagcgtggt
    25681 cgcggcagac accgcaaaca aaatctctac gccgcacatc gccgactttt gcaccatttc
    25741 ataactggca cgactggaaa ccagtaccgc accttgctgc cagctttccc cttcttgtga
    25801 ccggcggcct aacagtttgt ccagcgccac atggcgaccc acgtcttcat gcccgccgac
    25861 cagttcgcca gatggcaaca tccaggcagc ggcgtgagta caaccagtca gttgccccac
    25921 tggctgaaaa tcgttgagat gacgtaatgc atcatccagt ttgttgagat caaacgtctg
    25981 ggtgaacggt agcggctgca ccggttttcc gatgtcatta agttgctcca cgccgcatac
    26041 gccacagccc gtacgtcccg ccagcgcccg gcggcgctcc ttcaacccca taaagcggcg
    26101 gctggaaagc tcaatttgta cttcaagacc attacaggaa ggaacgacat ccatgccgaa
    26161 gatatcgcgc ggactttcta ttatcccctc ggaaagcgaa aaaccgagcg caaagtactc
    26221 aaggtctttg ggcgacgcca tcatcaccac atgcgaaatg ccgttgtaga ccagcgcaac
    26281 gggaacttct tccgcgacct catctaaccg tgggtgttgt aaatcatcac gccgccataa
    26341 ctcaatttga cgaacacctg tgatatttgt cacattttcg atttcttttc gctgtgtttt
    26401 tttcactttc tcttaaccgg ataagaacac acacaccaac attatggtat tctgttacaa
    26461 acccttcttg gatggaggga aattgagcca attctggacc tttgcggccc cttccgcaaa
    26521 gaaaaataac tcccactccc tgcacacgca gcaagcgaat gtaaatggga cgtgacaatg
    26581 tcgaaacaag gagcaatcca tgcaggtcag cagaaggcag ttctttaaga tctgcgctgg
    26641 cggtatg
//
LOCUS       DAMZET010000047        26644 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.047, whole genome shotgun sequence.
ACCESSION   DAMZET010000047 DAMZET010000000
VERSION     DAMZET010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.047"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..561
                     /gene="argO"
                     /locus_tag="POB34_002072"
     CDS             <1..561
                     /gene="argO"
                     /locus_tag="POB34_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HDI5919020.1"
                     /translation="VMNQGIRRQYHIMIALLCAISDLVLICAGIFGGSALLMQSPWLL
                     ALVTWGGVVFLLWYGFGAFKTAMSSNIELASAEVLKQGRWKIIATMLAVTWLNPHVYL
                     DTFVVLGSLGGQLDVEPKRWFALGTISASFLWFFGLAILAAWLAPRLRTAKSQRIINL
                     VVGCVMWFIALQLARDGIAHAQALFS"
     gene            654..1394
                     /locus_tag="POB34_002073"
     CDS             654..1394
                     /locus_tag="POB34_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HDI5919021.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            1561..2457
                     /locus_tag="POB34_002074"
     CDS             1561..2457
                     /locus_tag="POB34_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI5919022.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEGRQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHKPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTSH"
     gene            complement(2454..3932)
                     /gene="scpC"
                     /locus_tag="POB34_002075"
     CDS             complement(2454..3932)
                     /gene="scpC"
                     /locus_tag="POB34_002075"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HDI5919023.1"
                     /translation="METQWTRMTANEAAEIIQHNDMVAFSGFTPADSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYQDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(3956..4741)
                     /gene="scpB"
                     /locus_tag="POB34_002076"
     CDS             complement(3956..4741)
                     /gene="scpB"
                     /locus_tag="POB34_002076"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HDI5919024.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(4752..5747)
                     /gene="meaB"
                     /locus_tag="POB34_002077"
     CDS             complement(4752..5747)
                     /gene="meaB"
                     /locus_tag="POB34_002077"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HDI5919025.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DTIMPYCGNTLRLGVTGTPGSGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIINFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(5740..7884)
                     /gene="scpA"
                     /locus_tag="POB34_002078"
     CDS             complement(5740..7884)
                     /gene="scpA"
                     /locus_tag="POB34_002078"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HDI5919026.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEEVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(8088..8981)
                     /gene="argP"
                     /locus_tag="POB34_002079"
     CDS             complement(8088..8981)
                     /gene="argP"
                     /locus_tag="POB34_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HDI5919027.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            9123..9353
                     /locus_tag="POB34_002080"
     CDS             9123..9353
                     /locus_tag="POB34_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919028.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLANGELVRLFAPWSGIP
                     RLLYALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            9409..10068
                     /gene="rpiA"
                     /locus_tag="POB34_002081"
     CDS             9409..10068
                     /gene="rpiA"
                     /locus_tag="POB34_002081"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDI5919029.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            10324..11556
                     /gene="serA"
                     /locus_tag="POB34_002082"
     CDS             10324..11556
                     /gene="serA"
                     /locus_tag="POB34_002082"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDI5919030.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            11785..11844
                     /gene="ibsC"
                     /locus_tag="POB34_002083"
     CDS             11785..11844
                     /gene="ibsC"
                     /locus_tag="POB34_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HDI5919031.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            12177..12236
                     /locus_tag="POB34_002084"
     CDS             12177..12236
                     /locus_tag="POB34_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDI5919032.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(12337..12939)
                     /locus_tag="POB34_002085"
     CDS             complement(12337..12939)
                     /locus_tag="POB34_002085"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDI5919033.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(12960..13143)
                     /gene="ssrS"
                     /locus_tag="POB34_002086"
     ncRNA           complement(12960..13143)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="POB34_002086"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(13185..13514)
                     /gene="zapA"
                     /locus_tag="POB34_002087"
     CDS             complement(13185..13514)
                     /gene="zapA"
                     /locus_tag="POB34_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDI5919034.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            13682..14260
                     /gene="ygfB"
                     /locus_tag="POB34_002088"
     CDS             13682..14260
                     /gene="ygfB"
                     /locus_tag="POB34_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HDI5919035.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            14286..15611
                     /gene="pepP"
                     /locus_tag="POB34_002089"
     CDS             14286..15611
                     /gene="pepP"
                     /locus_tag="POB34_002089"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HDI5919036.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            15608..16786
                     /gene="ubiH"
                     /locus_tag="POB34_002090"
                     /gene_synonym="visB"
     CDS             15608..16786
                     /gene="ubiH"
                     /locus_tag="POB34_002090"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HDI5919037.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAVL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            16809..18011
                     /gene="ubiI"
                     /locus_tag="POB34_002091"
                     /gene_synonym="visC"
     CDS             16809..18011
                     /gene="ubiI"
                     /locus_tag="POB34_002091"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HDI5919038.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDTTLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQAGEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGGNLGFMDAAELVAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAAMMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(18085..18168)
                     /locus_tag="POB34_002092"
     CDS             complement(18085..18168)
                     /locus_tag="POB34_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919039.1"
                     /translation="MSKNTKSKIRDIRKYNAKTEVKLVYFK"
     gene            18459..19553
                     /gene="gcvT"
                     /locus_tag="POB34_002093"
     CDS             18459..19553
                     /gene="gcvT"
                     /locus_tag="POB34_002093"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HDI5919040.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            19577..19966
                     /gene="gcvH"
                     /locus_tag="POB34_002094"
     CDS             19577..19966
                     /gene="gcvH"
                     /locus_tag="POB34_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDI5919041.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            20084..22957
                     /gene="gcvP"
                     /locus_tag="POB34_002095"
     CDS             20084..22957
                     /gene="gcvP"
                     /locus_tag="POB34_002095"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HDI5919042.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            23223..23966
                     /locus_tag="POB34_002096"
     CDS             23223..23966
                     /locus_tag="POB34_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI5919043.1"
                     /translation="MAIALVTGGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(24023..25456)
                     /gene="bglA"
                     /locus_tag="POB34_002097"
     CDS             complement(24023..25456)
                     /gene="bglA"
                     /locus_tag="POB34_002097"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HDI5919044.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            25501..25812
                     /locus_tag="POB34_002098"
     CDS             25501..25812
                     /locus_tag="POB34_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDI5919045.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            25976..26635
                     /locus_tag="POB34_002099"
     CDS             25976..26635
                     /locus_tag="POB34_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDI5919046.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
BASE COUNT         6256 a         6724 c         7187 g         6477 t
ORIGIN      
        1 gtgatgaatc agggcattcg tcgtcagtac cacattatga ttgccttact ttgcgctatc
       61 agcgatttgg tcctgatttg cgcggggatt tttggtggta gcgcgttatt gatgcagtcg
      121 ccgtggttgc tggcgctggt cacctggggc ggcgtagtct tcttgctgtg gtatggtttt
      181 ggcgctttta aaacagcgat gagtagtaat attgagttag ctagtgccga agtcctgaag
      241 caaggcagat ggaaaattat cgccaccatg ttggcagtga cctggctgaa tccgcatgtt
      301 tacctagata cttttgttgt actgggcagc cttggcgggc agcttgacgt cgaaccaaaa
      361 cgttggtttg cgctcgggac aattagcgct tctttcctgt ggttctttgg tctggctatt
      421 ctcgcagcct ggctggcacc acgcctgcgc acggcaaaat cacagcgcat tatcaatctg
      481 gtagtgggat gtgtcatgtg gtttatagcc ttgcagctgg cgagagacgg tattgctcat
      541 gcacaagcct tgttcagtta ggcgctatct gatggaaaaa taaaacagag gcgctaagct
      601 tgcctccaga ggtcctgaat tttgggcaag tcaattaaca cggagagact aacgtgaagt
      661 tcaaagttat cgccctggcg gcattaatgg gtattagcgg gatggcagcg caggctaacg
      721 aattgccgga tggaccgcat attgtcacct ccggtacggc aagtgtggat gcggtgccag
      781 acattgccac tcttgcgatt gaagttaacg tggccgcgaa ggatgccgct acagccaaga
      841 aacaggcaga tgagcgcgtc gcacaataca tttccttcct tgaactcaat cagatcgcga
      901 aaaaagatat cagctcagcg aatttacgca cccagccaga ttatgattat caggatggta
      961 aaagtatcct gaaaggctac cgcgctgtga gaacggtgga agtcacgctc cgtcagttag
     1021 acaaactgaa ttccttgctg gatggcgcgc tgaaggcggg tcttaacgaa attcgttcag
     1081 tgtcgctggg cgtggcgcag ccggatgcct ataaagacaa agcgcgtaag gcagcgattg
     1141 ataacgcgat tcatcaggcg caggaactgg cgaacggctt tcatcgtaaa ctggggccgg
     1201 tatatagcgt gcgctaccat gtttccaact atcagcccag cccaatggtg cggatgatga
     1261 aagccgatgc cgcgccggtg tctgcccagg aaacttacga gcaggccgct attcagtttg
     1321 atgatcaggt cgatgtggtc ttccagttag aacctgtgga tcaacaaccc gctaaaacac
     1381 ctgcagcaca ataatacttg tagcccgacg tattcgtcgg gctaatgcct ttacccgata
     1441 ttgcctcctt tttctgtaat tttctgtaaa accatgacgt aatcacctat ctttgctcgc
     1501 ggtttcatca aggataggct gactgaaacg gttaagcgga caaggatgcg gggagggggg
     1561 atggacattt ttatttcgaa aaagatgcgc aatttcattt tattagcgca aaccaataat
     1621 attgcccggg cggcagaaaa aatccatatg acggcttcgc catttggtaa aagcattgcc
     1681 gcgctggaag aacaaattgg ctatacgcta tttacccgca aagataataa tatcagcctc
     1741 aataaagccg gtcaggaact gtatcaaaaa ctgttcccgg tttatcaacg actttctgcg
     1801 attgataatg aaatccataa ctcagggcgt cgttcacgag aaattgtgat tggcatagat
     1861 aatacttatc cgacaattat tttcgatcag ttgattagtc tcggagataa gtatgaaggc
     1921 gtgaccgccc agccagttga gttcagcgaa aacggcgtta tcgataatct ttttgaccgt
     1981 cagttagatt ttattatctc cccacagcat gtatctgcac gagttcagga actggaaaac
     2041 ctgactatca gtgaattacc accgctgcga ttgggctttc tcgtttctcg ccgttatgaa
     2101 gggcgtcaag agcaagagct gcttcaggag ctgccctggc tacaaatgcg ctttcaaaac
     2161 cgggctaact tcgaagcaat gattgatgcg aatatgcgac cttgtgggat caatcccacc
     2221 attatttatc gtccctacag ttttatggcc aaaatcagcg ccgtggagcg cggacatttt
     2281 ctgacggtga tcccgcattt tgcctggcga ctggtgaatc cggcaacgct gaaatatttt
     2341 gatgctccgc acaagccgat gtatatgcag gaatatcttt attcaatcag aaatcatcgt
     2401 tataccgcca cgatgcttca gcatattgct gaagatcgtg acgggacgag tcattaaccc
     2461 agcatcgagc cggttgcaat taaattacgg tgtaagtcga agacgtggct aagatcgtgg
     2521 tgaatatgtc cgccaggcgc attttccaga tagcgatgca gataatcctg atacatagga
     2581 tgtgcacaat tatcaatgat agtgcgggcg cgttgaagcg gggaaagacc gcgcagatcg
     2641 gcgatccctt gttcggtgat gatcactttg acgctgtgtt cgctgtgatc aacatggctg
     2701 cacattggca cgacggttga gatcttgcct tctttagcaa tcgacggggc cataaagatc
     2761 gacagatacg cgttgcgttc aaaatcaccg ctgccaccga tgccgttcat cagatcgacc
     2821 ccggctacgt gcgttgagtt ggcatgcccg taaatatcaa actccaggcc gacgttcaga
     2881 gcgatgacgc ccagacgacg gatgatttcc gggttattgg aaatctcctg cggacgcaac
     2941 acaatgcggc tggcaaagta atccatattg tcgtaaatct tgcgcaggga atcggccgag
     3001 attgtcaggc tggaggcgct ggccccgctg attttgccgg tttccagtaa atgcaccacc
     3061 gattcctgta gcacttccga atacatcata aacggaggaa tttccgggtt ttcccccaga
     3121 cgcgccatta ccgcattatt gatattgccc acgccacttt gcagcggcag aaattccggc
     3181 ggaatacgcc catgcgccat ttcctgcaac aagaacgtga ccacgttatc ggcaatctgc
     3241 tggcacatgg gattttgctt atccagcata ttaccggcgt cgggcaagtt ggtttccacg
     3301 acggcgacaa tctttttcgg atcgatttgc acatagcggg taccgacgcg atccattgca
     3361 tggaagatcg acacgctatt gcgccgcggt ggcgcgccag gaatcacaat atccgccagt
     3421 tctgcaacgc gcggatcgtg atagtgattg agttcaatga tcactttctt cgcccgcagc
     3481 agccaggtcg gcgcattacc gatcccgctg gttaaccaga ctcgaccatc cggtgccagt
     3541 gccgatgctt caatgacggc aacatcaatg tcgccgaaga aaccgtaatt gaccatttgc
     3601 gccacttcgc tcaaatgcag gtcaacgaaa ctcaccgcgc cctgattgat ctttttacgt
     3661 aaaccggacg atgtttgata tggcgcacgc caggaaacag catcggcgtc agaaagtaca
     3721 tcgtcagcgg cggcgctgat tgacgcaccc gtcagaaggc gaatttgata cggctttttg
     3781 gcctcatgct gttcgttagc tctgcgggca atcgcggtgg gtagggcttt cggcgaatcc
     3841 gccggggtaa agccgctaaa tgccaccatg tcgttatgct ggataatttc tgccgcttca
     3901 ttggcggtca tccttgtcca ctgagtttcc atttttactc cttcgttcgc agggattaat
     3961 gaccaacgaa attaggttta cgtttttcga ggaaagcgtt catcccttcc tggtaatctt
     4021 cgctgtcata caccgcgcgg cgcatcccct gaatacgttc aaattcatcg gagttcatgg
     4081 tgtgtgcttc gcccagtaca cgcagctctt ctttgataac ggcaatggct aacggcgctt
     4141 tctcagagat gtggtgcgcc atttgtaagg tgaaatcttc cagttcttcc acttccacaa
     4201 catggttgag gatgccgaca gccagcgcgc gctgggcggt gattggcgaa gcggtaaaaa
     4261 tcagctcttt gacaatgtgg aagcccgcgt cgcgggtcag gttgtgaatg ccgaccaggt
     4321 tatacgggac gccgaggttt acaggcgtca ttgagaaggt tgaggtactg gcggcgatga
     4381 tcagatcgga actcatgatc atttcaaatg cgccacccca aacactacct tccaccatcg
     4441 aaatgatcgg tttcgggaat ttttggatca tgcgggtgat ttgacgcaat ggatcatcat
     4501 aggagagcgg atcgcgaccg ccagacggca gttcgtgaat atcgtggcct gcggagaaga
     4561 ctttggatcc actcggcgcg cgcaaaatga tacagcgaat ttccggccgg ttgagatcgc
     4621 ttaatgcctg cataagatca tcaataaaga ctttacttaa ggcattaagt ttgcggccat
     4681 agttaaactc aatgaccgcc actttgttga tagtgacaac gttaacatac tgataagaca
     4741 taaaaattcc tttaatcaaa atattgcgtc tggataaatt cactgagctg ccgcaggccg
     4801 gtgcgcggtg agagcgtatt gtttttgacc gctaaaagcg tctggcggta atagcgatcg
     4861 aaatcttcat tcgcgaacag gtgattcagt acttcttctt cggtctgctt acgcagccat
     4921 tccaccgatt gttgttgccg cacttgttgt aaacgaccac tggcagttag cgcggttttg
     4981 aagttgatga tggcgtgcca gatctcatcg attccacgtt tttccagtgc gctacaagtc
     5041 agaacccgtg gctgccattc gtcgtattta cgtcgcagaa tatgcagggc actctcgtac
     5101 atatgccggg caatggcgac attggtatgg ttatcgccat cgtctttgtt gataacgagc
     5161 agatcagcca cttccatcag ccctttttta atgccctgca gatcatcgcc gccaccggca
     5221 atttgcaacg agataaaaca gtccaccatg cgggcgactt ccgtttccga ctgcccgacg
     5281 ccaaccgttt cgacaatcac tacgtcataa cccgccgctt cgcataacag cattaattcc
     5341 cgcgctcgct gactggcacc gcccagatga ccggaggatg gtaccgggcg aataaacgcc
     5401 gcttcggcac gcgccaggtc attcatgcgg gttttatccc cgagaatgct accgccagtg
     5461 accgggctgc tgggatcgac cgcaataacc gcgaccttta atccctctcg aatcaacaac
     5521 atgccaaagg cctcaagaaa ggtacttttc cccgagccgg gggtgccggt aacgcccagt
     5581 cgcagggtgt taccgcagta cggcataatg gtatcaagca gctgcgtact tagtgcctga
     5641 tgacgcgggt gacggctttc caccagcgtc atggcctggg cgagtgtggc acgctcaccc
     5701 tgacgtaagc ggcgaatact ttctgccagc gtggcttcat taatcatgat gctggcttat
     5761 cagattcagt acgtcgcgca cactgtcgag cataggtgta cctggaccat aaatcgccgc
     5821 cacgccgcgc tcttgcagga aggcgtaatc ctgcggcgga atgacgccac ccgcgaccac
     5881 gcagatatct tcgcgtcccc attttttcag cgcttcgacc agttccggga tcagcgtttt
     5941 atgaccggca gccagtgagg atgcgcccac tacgtgaacg tcgttttcta cggccaggcg
     6001 ggcgatctct tcaggtgtag agaacatcgg gcttaaatct acgtcgaaac cgagatcgga
     6061 ataggcgctg gcgatcactt tcgcgccgcg atcgtgtcca tcctggccca tcttagcgat
     6121 cagaatgcgc gggcgacgac cattgtcggc aaggaactgc tccgtttgcg caacaatggc
     6181 atcgaactcg gaggccgatt tctccgactg atgatagctt tgcgcaatta cgccggtaac
     6241 acactggctt ggcaccagat aacggtcgaa agcggcttcc agcgcatcgg aaatttcacc
     6301 cagggtggcg cgaacgcgag cggcattaac agcggcagcc agcaggtttt cgttatgctg
     6361 tgcggcgtga gtcagggcgt tcaacgcggc ggttacggcg gcatcatcac gggtggcgcg
     6421 aatgcgttcc agcgaagcaa tttgctcgtt acgcaccatc acgttgtcga tctcaagtac
     6481 atcggtttcg tcttcgtgat ccagtttgta cttgttgaca ccaacgatga cacgcttgcc
     6541 ctggtcgatc agcgactgtt cgcgcgctga ggcctcttcg atcattcgtt ttggcagacc
     6601 tgcttcgatc gctttcgcca tgccaccggc ttcgtcgatc tgttggataa tagctctggc
     6661 ttgtttgacg atttgatcgg tcagcgactc aatgtaatag gatccggcca gtggatcgac
     6721 ggtgcggcag agttctgatt cttcctggat gatgatctgg gtgttgcggg caatgcgtgc
     6781 tgagaaatcg gtaggcaaac caagcgcttc gtcaaaggcg ttggtatgca gtgactgagt
     6841 accgcccagc gtcgcagcca gcgcttcaat ggtggtgcgg ataacgttgt tatacggatc
     6901 ctgttcagtc aggctccagc ctgaggtctg gcagtgggta cgcagcgcca gtgatttcgg
     6961 gtcctgtgcg ccaaatccac tgacctcttc gctccataaa taacgtgccg cacgcaacat
     7021 ggcgacgttc ataaacagat ccatgccgat gccgaagaag aacgacaggc gaggagcgaa
     7081 gtcatcaatt ttcagtccgg cagagattgc tgctttgatg tactcaatcc catcagcgag
     7141 cgtaaatgct acctgctgca cgcagttggc acccgcttca cccatgtggt aaccgctgat
     7201 actgatggta ttaaatcgcg gcatgttgcc ggaacaccag gcgatgatgt cggcgataat
     7261 gcgcattgac ggttttggcg ggtaaatata ggtgttgcgg cagaggtact ctttgagaat
     7321 atcgttttga atggtgccgg tcagtttatc aggtgtaaca ccttgctctt ctgcggcgac
     7381 gatataaaac gccagtactg gtagcactgc gccattcatg gtcatcgaaa ccgacatttt
     7441 atccagcggg atctggtcga acaggacttt catatcttcc acggtgtcga tagcgacgcc
     7501 cgctttgccg acgtcacccg ccacgcgtgg gttatcggag tcgtagccac ggtgggtggc
     7561 aaggtcaaac gcaacggaaa gacctttttg cccggcggcc aggttacggc gataaaaagc
     7621 gttggactct tttgctgttg aaaaaccagc atactgacgg atggtccacg gttgggcggt
     7681 atacatagtg gcacgcgggc cacgaacgta gggcggcaaa ccaggaaggg tacctgtcac
     7741 ctccagatta tcgagatcgg cttcggtata cagcggcttg atggcgatcc cttccgcggt
     7801 ttgctgaacc agcgagtcga cagttttctc ccgacggctc aattccttgt tggcaagctg
     7861 ttgccactcc tgctcgttag acattatttg ctccgtaaaa cgcaatcaaa atgagggtat
     7921 ttgcgcttac ggtgagcttt ttacgtgggt ttgtcgccag caaaaaaaat gcattcagac
     7981 gtcgtttttt gccgcctgag atttgtctat tttgtgatgt gattcaagga atacaaaaag
     8041 aagtgaggac aacgcctgat atgtgctatc aggcattatt tgatggatta atcctgacga
     8101 aggactttgt gaccataatc gagtaacgca tcagtgactt tacgcatcat gcggctttca
     8161 ggagcaaagc ggtgccagta gagcatccgt cgttgaaata gcccaggcgt taagtcaatc
     8221 agttcaccgc tggccagctc tttctcgatt tgcaggtgcg ggatcataca gcaggtggtg
     8281 ccctggcgag caagttgtac gaacgcttct gaagaattaa cgatatggca gggcacgctg
     8341 cctggaggca gatcgaagtt ttgctgcaaa aaggcctggt gcatatcgtc aagatggtca
     8401 aacgcgacca ctggcgcttt cagtaatgcc gaacgcgtta cgccgttagg gaaatatttt
     8461 tcggcaaagg gttttgagct gacgaacaga tagtcgagcg caccaagttt atcgacaaga
     8521 caactcggca gcgcctgatg ttgaatactc accgcgccga ccacttcgcc gcggcgcaga
     8581 cgttcctgag tgcgggtttc atcttctacc tgcaagttga ggcggatagg cgaatcagcc
     8641 aacacaggag ccagtgcagg aagcaaccac gtcgccagac tgtcggcgtt gaccgccagt
     8701 gaaagcagca gcggagtcga accggtttgt tcatcgccca gccactcttc ttccagcaac
     8761 tccacctggc gcagcagtgc cagcagtttt tgcccttgtt ccgtcgggcg cggcggtacg
     8821 gtacgcacca acagcggctg cccgaacata ttttccagtt gcttaatgcg ctgtgagacg
     8881 gctgattgtg taatgcacag cttttgtgcc gcgcgctcaa atcctcgttc acgtatcacc
     8941 gcatccagtg cctgtaatgt tctgtagtcc gggcgtttca ttgctgttgt ctgctcctgg
     9001 taaatgcttt ttcctgcact atgacacaat tttgtgtcag gttgcatata ccgaagccgt
     9061 cggcgggtaa catttgtatt cgtggttaaa cgcgcagctt aaaagatcag ggatttgcgc
     9121 taatgcattt cgctcagcga gttcgcgccc tggttgtgtt gaatggcgtg gcgttattgc
     9181 cccaatttgc ctgtaaacag gggcttgcga acggtgaact ggtgcgcctg tttgcaccgt
     9241 ggagcggcat acccagattg ttatatgcct tatttgcggg gcgaaagggg atgcctgcca
     9301 ttgcgcgata ttttatggat gagttaacca cgcggcttgc caacggggtc tgaatcgctt
     9361 tttttgtata taatgcgtgt gaaatttcat accacaggcg aaacgatcat gacgcaggat
     9421 gaattgaaaa aagcagtagg atgggcggca cttcagtatg ttcagcccgg caccattgtt
     9481 ggtgtaggta caggttccac cgccgcacac tttattgacg cgctcggtac aatgaaaggc
     9541 cagattgaag gggccgtttc cagttcagat gcttccactg aaaaactgaa aagcctcggc
     9601 attcacgttt ttgatctcaa cgaagtcgac agccttggca tctacgttga tggcgcagat
     9661 gaaatcaacg gccacatgca aatgatcaaa ggcggcggcg cggcgctgac ccgtgaaaaa
     9721 atcattgctt cggttgcaga aaaatttatc tgtattgcag acgcttccaa gcaggttgat
     9781 attctgggta aattcccgct gccagtagaa gttatcccga tggcacgtag tgcagtggcg
     9841 cgccagctgg tgaaactggg cggtcgtccg gaataccgtc agggcgtggt gaccgataat
     9901 ggcaacgtga tcctcgacgt ccacggcatg gaaattcttg acccgatagc gatggaaaac
     9961 gccataaatg cgattcctgg cgtggtgact gttggcttgt ttgctaaccg tggcgcggac
    10021 gttgcgctga ttggcacacc tgacggtgtc aaaaccattg tgaaatgatc tgacggggga
    10081 acctcccccg ttaaaaaaat tctcttcatt aaatttggtg acatgtgtca cgcttttacc
    10141 aggcaattgt cgattgctct aaataaatcc tctaaaccag catattcatc caagaattac
    10201 ctttgcgtga tatttcctca acatcgcgac gcaaacgttc atattgccgc aatattattt
    10261 tttgatatgt tgaaaggcgg atgcaaatcc gcacacaaca tttcaaaaga caggattggg
    10321 taaatggcaa aggtatcgct ggagaaagac aagattaagt ttctgctggt agaaggcgtg
    10381 caccaaaagg cgctggaaag ccttcgtgca gctggttaca ccaacatcga atttcacaaa
    10441 ggcgcgctgg atgatgaaca attaaaagaa tccatccgcg atgcccactt catcggcctg
    10501 cgatcccgta cccatctgac tgaagacgtg atcaacgccg cagaaaaact ggtcgctatt
    10561 ggctgtttct gtatcggaac aaaccaggtt gatctggatg cggcggcaaa gcgcgggatc
    10621 ccggtattta acgcaccgtt ctcaaatacg cgctctgttg cggagctggt gattggcgaa
    10681 ctgctgctgc tattgcgcgg cgtgccggaa gccaatgcta aagcgcaccg tggcgtgtgg
    10741 aacaaactgg cggcgggttc ttttgaagcg cgcggcaaaa agctgggtat catcggctac
    10801 ggtcatattg gtacgcaatt gggcattctg gctgaatcgc tgggaatgta tgtttacttt
    10861 tatgatattg aaaacaaact gccgctgggc aacgccactc aggtacagca tctttctgac
    10921 ctgctgaata tgagcgatgt ggtgagtctg catgtaccag agaatccgtc caccaaaaat
    10981 atgatgggcg cgaaagagat ttcgctaatg aagcccggct cgctgctgat taatgcctcg
    11041 cgcggtactg tggtggatat tccggcgctg tgtgatgcgc tggcgagcaa acatctggcg
    11101 ggggcggcaa tcgacgtatt cccgacggaa ccggcgacca atagcgatcc atttacctct
    11161 ccactgtgtg aattcgacaa cgtccttctg acgccacaca ttggcggttc gactcaggaa
    11221 gcgcaggaga atatcggcct ggaagttgcg ggtaaattga tcaagtattc tgacaatggc
    11281 tcaacgctct ctgcggtgaa cttcccggaa gtctcgctgc cactgcatgg tggtcgtcgt
    11341 ctgatgcaca tccacgaaaa ccgtccgggc gtgctaactg cgctgaacaa aatcttcgcc
    11401 gagcagggcg tcaacatcgc cgcgcaatat ctacaaactt ccgcccagat gggttatgta
    11461 gttattgata ttgaagctga cgaagacgtt gccgaaaaag cgctgcaggc aatgaaagct
    11521 attccgggta ccattcgcgc ccgtctgctg tactaattcc ccttctctga aaattaacgg
    11581 gcaggtcact gacttgcccg tttttttatc ccttctccac accgttgccg cgttgcgcat
    11641 tctactttgc gagtctgctc cagaaaataa atcaaagtca gatcaatgcg ttgtgtttat
    11701 ttaatgggga tttacgatgg cgggacagca tggggctgta acggtaaagc cctcaccgaa
    11761 gcgagggctt aaaggagaag ggttatgatg cgacttgtca tcatactgat tgtactgtta
    11821 ctgataagtt tcggagctta ttaacagtca gtctcagggg aggagtcatc ctcccttacc
    11881 cttactcact aaattaggtc aaagaatcaa cgatgtcaat cagggcgatg cggggtgcat
    11941 cgccctgaac cgaacttttt gatagccact tcatcgccca cgtttactgc cgtaatagat
    12001 cctttttccc aaaaattatc tctgttttgc atggggtttt tcgagcacgc tcgaagaaaa
    12061 gatttaaagg tagatcaatg cgttgtggtt atttaaagag agcttacgat agctgggcag
    12121 catggggctg taacggtaaa gccctcaccg aagcgagggc ttgaaggaga agggttatga
    12181 tgcgacttgc catcatactg attgtactgt tactcataag tttcccagct tattaacagt
    12241 cagtcacagg ggaggagcaa tcctccctta cccttactca ctaaattagg ttaaagaatc
    12301 aacgatgtca atcagggcga tgcaggtgta tcgcccttac cactcccaga ctttcgacgg
    12361 tgtaaccacc gcaggaagag ggatatccca ctcttcaacg gggagttttt ccaccaactg
    12421 acaatcatgc gcataaccca ccggttgcgt tttatagtgc tgccagtttt gtaaggtccg
    12481 atcataaaaa ccaccgccca ttcccaggcg ctgaccgtac tcatcaaagg cgaccagcgg
    12541 tgtgatcagc acgtctaatc gggaaagggg tagcacgtca cgcacatcca gttttggctc
    12601 atggattttc aacctgttca tcaccagttc gctttgcgga tggtaattca ggaacagcaa
    12661 attaccggca ctaaagggat gcaaaactgg aagataaacg cgcttaccgg cgcgccagag
    12721 ttgttctatg agtggctggg tgtcgagttc gccatcaaaa gagaggaata cagcgaccgt
    12781 atgtgccatc accaccgggg gataagtcat catccgggta gcggcttgtt gacccatttc
    12841 ttgctgttgt tccggcgtta acgcacgacg acgttgccga atcattttgc ggatttcttg
    12901 tcgggataat gtcagtggga gttctggtag ttgcgtcatg gctggtgcca gataagaagg
    12961 gaatctccga gatgccgccg caggctgtaa cccttgaacc cttggttcaa ggtgaatgtg
    13021 tcgtcgcagt tttaaggctt ctcggacgga ccgagcatgc tcaccaaccg cggagcgcca
    13081 cattcttgtg gtatgaaata tcggctcagg ggactggccc gcttgcgaac atctcagaga
    13141 aattttgtct tcacggttac tctaccacag taaaccgaaa agtgtcattc aaagttttgg
    13201 ttagtttttt cggtgatgcg accttgttca agtaacgctt gttctatggt ctgctgcagc
    13261 atccgaatac gctgttccat acttgccgcg tagtcacgag tctttgcttt ttcttgcgct
    13321 aactcatagc tgatattcaa tgcggcaatg aagaccaact gttcagtatt tgtgactcta
    13381 gtgcgttctt tcagatcttg caaccgttgg ttcagatcgt ccgctgcctg attcaacgca
    13441 tccctttggt caggcgggca gttcacacgc agtgaacggc caaaaatttg gatatcgacg
    13501 ggttgtgcag acatgccacc ttcctgctga ttgactgcgc tgccttcgtc tttagaccca
    13561 tggtctgcga aggggcgaca ctatagctac cctgatgaga agagacaagc ccttttctgg
    13621 tccaccaggg cccaaagtgg tagcatatca tgaatattcc tccctttgac gacgaatgct
    13681 tatgtctata cagaacgaaa tgcctggtta caacgaaatg aaccagtatc tgaaccaaca
    13741 agggacgggt ctgacccctg ctgagatgca tggtttaatc agtgggatga tatgtggcgg
    13801 taacgatgac agctcatggc taccgctact tcacgacctg acgaacgaag gcatggcttt
    13861 cggtcatgag ctggcacagg cactgcgtaa aatgcactct gccaccagcg atgccctgca
    13921 ggatgacggc ttcctttttc agctttatct gcctgatggc gatgatgtca gcgttttcga
    13981 tcgggctgat gcattggcag gttgggtcaa tcacttcctg cttggtcttg gcgttacgca
    14041 accgaagctg gataaagtga ccggcgaaac cggtgaagct atcgacgatc tgcgtaacat
    14101 tgcgcaactg ggttacgacg aagacgaaga tcaggaagag cttgaaatgt cgcttgaaga
    14161 gatcatcgaa tacgttcgtg ttgccgcgct gttatgccac gacaccttta ctcatccgca
    14221 accgaccgcg ccagaagtac aaaaaccgac tctacactaa aaacaaaaaa cgtaaggaga
    14281 gtgttatgag tgagatatcc cggcaagagt ttcagcgtcg ccgtcaggcc ctggtggagc
    14341 aaatgcaacc cggcagcgcc gcgctgattt ttgctgcacc agaagtaaca cgtagcgccg
    14401 acagcgaata cccctatcgt cagaacagtg acttctggta cttcaccggc tttaacgaac
    14461 cggaagcggt gctggtgctg attaaaagcg atgacactca taaccacagc gttctgttta
    14521 accgcgttcg cgacctgacg gcggagatct ggtttggccg tcgcttaggc caggatgccg
    14581 cgccagagaa actgggcgtt gaccgcgcac tggcattcag cgaaatcaat cagcaacttt
    14641 atcaactact taacggcctg gatgtggttt accatgccca gggcgaatat gcatatgctg
    14701 atgaaatcgt gaacagtgcg ctggaaaaac tgcgtaaagg ttcgcggcaa aatctcaccg
    14761 caccggcaac gatgatcgac tggcgtcctg ttgttcatga aatgcgcctg ttcaaatcgc
    14821 cagaagagat tgccgtactc cgccgcgcgg gagaaatcac cgccatggca catacacggg
    14881 cgatggaaaa atgccgtccg ggaatgttcg agtaccatct ggaaggcgaa attcaccacg
    14941 aatttaaccg ccacggtgcg cgctatccgt cctataacac cattgtcggc agcggtgaaa
    15001 acggctgcat tctgcactac accgaaaacg agagtgaact gcgcgacggt gacctggtgt
    15061 tgattgacgc gggttgtgaa tacaaaggtt acgcgggcga tattacccgc accttcccgg
    15121 tcaacggcaa attcacccag gcccagcgtg aaatctacga cattgtgctg gagtctctcg
    15181 aaaccagcct gcgcctgtat cgtccgggaa cttccattct ggaagtcact ggtgaagtgg
    15241 tgcgcatcat ggttagcggc ctggtaaaac tcggcatcct gaaaggtgat gttgatgaac
    15301 tgatcgctca gaacgcccat cgtcctttct ttatgcatgg ccttagccac tggttaggac
    15361 tggatgtcca tgacgttggc gtttatggtc aggatcgctc gcgcattctg gaaccgggga
    15421 tggtactgac cgtagagcct ggtctgtata tcgcgccgga tgcggatgtg ccagaacaat
    15481 atcgcggtat cggcattcgt attgaagacg acattgtgat taccgaaacc ggcaacgaaa
    15541 acctcaccgc cagcgtggtg aaaaagccgg aagaaatcga agcgttgatg gcagctgcga
    15601 gaaagcaatg agcgtaatca tcgtcggtgg cggcatggcg ggcgcgacgc tggcgctggc
    15661 tatttcccgg ttaagtcacg gggcgctgcc ggtacatttg attgaagcga ccgcgccaga
    15721 gtcacatgct catccgggct ttgatggacg agcgatagcg ctggcggcgg gtacctgtca
    15781 gcaactggcg cgcatcggcg tctggcaatc tctggcggat tacgcaaccg ccatcactac
    15841 cgtgcatgtc agcgatcgtg gtcacgctgg atttgtcacc ctcgccgcag aagattacca
    15901 actggcggtg ctgggacagg ttgtcgaatt gcacaatgtc gggcaacggc tgtttgcatt
    15961 gctgcgtaaa gcacctggcg taacgctgca ttgccctgat cgcgtggcta acgttgcccg
    16021 tactcagagt cacgttgaag tgacgctgga gagtggcgag acgctgacgg gccgcgtgct
    16081 ggtagcagct gatggcaccc attcagcgtt agccaccgcg tgcggcgttg actggcagca
    16141 ggagccttac gaacaactgg ccgtgattgc caacgttgcg acttccgttg cgcacgaagg
    16201 ccgcgctttt gaacgcttca cgcaacatgg cccgctggcg atgttgccga tgtctgacgg
    16261 acgctgttcg ctggtctggt gtcatccact ggaacggcgc gaagaggtgc tgtcgtggag
    16321 cgacgagaag ttttgccgtg aactccagtc ggcctttggc tggcgacttg gaaaaattac
    16381 ccacgccggt aaacgcagtg cttatccgct ggcgttaacc cgcgccgcca gagctattac
    16441 ccatcgtacc gtgctggtgg gcaatgcggc gcaaactctg cacccgattg ccgggcaagg
    16501 gtttaacctc ggtatgcgag atgtcatgag ccttgcggaa accctgactc aggcgcatga
    16561 gcgcggagaa gacatcggtg attacggcat attgtgccgc tatcagcagc gtcgacagag
    16621 cgatcgcgaa gcaaccatcg gcgtcacgga cagcctggta catctttttg ccaaccgttg
    16681 ggcaccgctg gttgtcgggc gcaacatcgg actgatgacg atggaattat tcaccccggc
    16741 acgcgatgtg ctggcgcagc gcaccctcgg ttgggtggcg cgttgaaggc ggttttaagg
    16801 aataaacaat gcaaagtgtt gatgtagcca ttgttggtgg cggcatggtg gggctggcgg
    16861 ttgcctgcgg cttacagggg agcggcttac gcgttgccgt actggagcag cgcgtaccgg
    16921 aacctctggc ggcggatgca ccaccacaac tgcgcgtttc ggctatcaat gccgccagcg
    16981 aaaaattact cacccgtctt ggcgtctggc aggacattct ctctcgtagg gccagttgtt
    17041 atcacggtat ggaagtgtgg gacaaagaca gttttggtca catttcgttt gacgatcaaa
    17101 gcatgggcta tagccatctt gggcatatcg ttgaaaactc agtgattcac tacgcgctgt
    17161 ggaacaaagc gcagcagtcg tcagatacca ctctgttggc ccccgcagaa ttacagcagg
    17221 tcgcctgggg agaaaacgaa accttcctga cgctgaaaga tggcagtatg ttaacggcgc
    17281 gtctggtgat tggcgcggac ggcgctaatt cctggttgcg caacaaagcc gatattccgc
    17341 tgactttctg ggattatcag catcacgcgc tggtagcgac cattcgcacg gaagaaccgc
    17401 atgatgcggt ggcgcggcag gttttccatg gcgaaggcat tctggccttt ttaccgctta
    17461 gcgatccgca tctttgctcg attgtctggt cactgtcgcc agaggaagcg cagcgtatgc
    17521 agcaggcagg ggaagacgaa tttaatcgcg cgttaaatat cgcttttgat aatcgcctgg
    17581 gcttatgcaa ggttgagagc gcgcgtcagg tgttcccact gacggggcgt tatgcgcgcc
    17641 agtttgccgc gcaccgtctg gcgctggtgg gcgacgccgc gcataccatt cacccgctgg
    17701 cggggcaggg gggaaatctc ggctttatgg atgctgcaga gctggttgct gaactgaaac
    17761 ggttgcatcg tcagggtaaa gacatcgggc agtacattta tctgcgtcgc tatgagcgta
    17821 gccgcaagca cagtgcggcg atgatgctgg ctggtatgca gggattccgc gatctgtttt
    17881 ccggtaccaa tccggcgaaa aaactgctgc gtgatattgg tctgaaactg gccgacacgc
    17941 ttcctggcgt taaaccacaa cttatccgcc aggcaatggg attaaacgat ttgcctgaat
    18001 ggctgcgtta aaaatttctc ttctgctgtt tatttgatac ccatcacact ttcatctccc
    18061 ggttttttcg ccgggagatt ttcctcattt gaaataaact aatttcacct ccgttttcgc
    18121 attatatttt ctaatgtcac gaatttttga tttagtgttt tttgacattt tttcagctct
    18181 taatattgtc ttattcaaat taactttctc atcacatcat ctttatataa aaacttgtgt
    18241 attttttggt tttttattct gttgcgattt tggcgttttt taaccataag ctaatgtgat
    18301 gatcaatttt accttatggt taacagtctg tttcggtggt aagttcaggc aaaagagaac
    18361 gattgcgttg gggaccggga gtggctccga tgctgggttt cgtggtgata atttcactat
    18421 gaaaaagttg tcagccccgc ttattcaatg aggacaagat ggcacaacag actcctttgt
    18481 acgaacaaca tacgctttgc ggcgctcgca tggtggattt ccacggctgg atgatgccgc
    18541 tgcattacgg ttcgcaaatc gacgaacatc atgcggtacg taccgatgcc ggaatgtttg
    18601 atgtgtcaca tatgaccatc gtcgatcttc gcggcagccg cacccgggag tttctgcgtt
    18661 atctgctggc gaacgatgtg gcgaagctca ccaaaagcgg caaagccctt tactcgggga
    18721 tgttgaatgc ctctggcggt gtgatagatg atctcatcgt ctactacttt actgaagatt
    18781 tcttccgcct cgttgttaac tccgccaccc gcgaaaaaga cctctcctgg attacccaac
    18841 acgctgaacc tttcggcatc gaaattaccg ttcgtgatga cctttccatg attgccgtac
    18901 aagggccgaa tgcgcaggca aaagctgcca cactgtttaa tgatgctcag cgtcaggcgg
    18961 tggaagggat gaaaccgttc tttggcgtgc aggcgggcga tctgtttatt gccaccactg
    19021 gttataccgg tgaagcgggc tatgaaattg cgctgcccaa tgaaaaagcg gcggatttct
    19081 ggcgcgcgct ggtggaagcg ggcgttaagc catgcggcct gggcgcgcgt gacacgctgc
    19141 gtctggaagc gggcatgaat ctttatggtc aggagatgga cgaaactatt tctcctttag
    19201 ccgccaacat gggctggact atcgcctggg aaccggcaga tcgtgacttc atcggtcgtg
    19261 aagccctgga agtgcagcgt gagcatggca cagaaaaact ggttggtctg gtgatgaccg
    19321 aaaaaggcgt gctgcgtaat gaactgccgg tacgttttac cgatgcgcag ggcaaccagc
    19381 atgaaggcat tatcaccagc gggactttct ccccgacgct gggttacagc attgcgctgg
    19441 cacgcgtgcc ggaaggtatt ggcgaaacgg cgattgtgca aattcgcaac cgtgaaatgc
    19501 cggttaaagt gacgaaacct gtttttgtgc gtaacggtaa agccgtcgcg tgatttactt
    19561 ttttggagat tgattgatga gcaacgtacc agcagaactg aaatacagca aagaacacga
    19621 atggctgcgt aaagaagccg acggcactta caccgttggt ataaccgaac atgcccagga
    19681 gctgttaggc gatatggtgt ttgttgacct gccggaagtg ggcgcaacgg ttagcgcggg
    19741 cgatgactgc gcggttgccg aatcagtaaa agcggcgtca gacatttatg cgccagtaag
    19801 cggtgaaatc gtggcggtaa acgacgcact gagcgattcc ccggaactgg tgaacagcga
    19861 accgtatgca ggtggctgga tctttaaaat caaagccagc gatgaaagcg aactggaatc
    19921 actgctggat gcgaccgcat acgaagcatt gttagaagac gagtaacggc tttattcctc
    19981 ttctgcggga gaggatcagg gtgaggatat ttacgcctca ccctcactct cttcgcaagg
    20041 agagaggttc acaattcact gcacgtttca ggaaccatcg ctcatgacac agacgttaag
    20101 ccagcttgaa aacagcggcg cttttattga acgccatatc ggaccggacg ccgcgcaaca
    20161 gcaagaaatg ctgaatgccg ttggtgcaca atcgttaaac gcgctgaccg gccagattgt
    20221 gccgaaagat attcagcttg cgaccccgcc gcaggttggc gcaccggcga ccgaatacgc
    20281 cgcactggca gaactcaagg ctattgccag tcgcaataaa cgcttcacgt cttacatcgg
    20341 catgggttac accgccgtgc agctaccgcc ggttatcctg cgtaacatgc tggaaaatcc
    20401 gggctggtat accgcgtaca ctccgtatca acctgaagtc tcccagggcc gccttgaagc
    20461 actgctcaac ttccagcagg taacgctgga tttgactgga ctggatatgg cctctgcttc
    20521 tcttctggac gaggccaccg ctgccgccga agcaatggcg atggcgaaac gcgtcagcaa
    20581 actgaaaaat gccaaccgct tcttcgtggc ttccgatgtg catccgcaaa cgctggatgt
    20641 ggtccgtact cgtgccgaaa cctttggttt tgaagtgatt gtcgatgacg cgcaaaaagt
    20701 gctcgaccat caggacgtct tcggcgtgct gttacagcag gtaggcacta ccggtgaaat
    20761 tcacgactac actgcgctta ttagcgaact gaaatcacgc aaaattgtgg tcagcgttgc
    20821 cgccgatatt atggcgctgg tgctgttaac tgcgccgggt aaacagggcg cggatattgt
    20881 ttttggttcg gcgcaacgct tcggcgtgcc gatgggctac ggtggcccac acgcggcatt
    20941 ctttgcggcg aaagatgaat acaaacgctc aatgccgggc cgtattatcg gtgtatcgaa
    21001 agatgcagct ggcaataccg cgctgcgcat ggcgatgcag actcgcgagc aacatatccg
    21061 ccgtgagaaa gcgaactcca acatttgtac ttcccaggta ctgctggcaa acatcgccag
    21121 cctgtatgcc gtttatcacg gcccggttgg cctgaaacgt atcgctaacc gcattcaccg
    21181 tttgaccgat atcctggcgg caggcctgca acaaaaaggt ctgaagctgc gccatgcgca
    21241 ctatttcgac actctgtgtg tggaagtggc cgacaaggcg ggcgtactgg cgcgtgccga
    21301 agcggctgaa atcaacctgc gtagcgatat cctgaacgcg gttgggatca cccttgatga
    21361 aaccaccacg cgtgaaaacg taatgcagct tttcagcgtg ctgctgggcg ataaccacgg
    21421 gctggagatc gacacgctgg acaaagacgt ggctcacgac agccgctcta tccagcctgc
    21481 gatgctgcgc gacgacgaaa tcctcaccca tccggtgttt aatcgctacc acagcgaaac
    21541 cgaaatgatg cgctatatgc actcgctgga gcgtaaagat ctggcgctga atcaggcgat
    21601 gatcccgctg ggttcctgca ccatgaaact gaacgccgcc gccgagatga tcccaatcac
    21661 ctggccggaa tttgccgaac tgcacccgtt ttgtccgccg gaacaggctg aaggttatca
    21721 gcagatgatt gcgcagctgg ctgactggct ggtgaaactg accggttacg acgccgtttg
    21781 tatgcagccg aactccggcg cacagggcga atacgcgggc ctgctggcga ttcgtcatta
    21841 tcatgaaagc cgcaacgaag ggcatcgcga tatctgcctg atcccggctt ctgcgcacgg
    21901 aactaacccc gcttctgcac atatggcagg aatgcaggtg gtggttgtgg cgtgtgataa
    21961 aaacggcaac atcgatctga ctgatctgcg cgcgaaagcg gaacaggcgg gcgataacct
    22021 ctcctgtatc atggtgactt atccttctac ccacggcgtg tatgaagaaa cgatccgtga
    22081 agtgtgtgaa gtcgtgcatc agttcggcgg tcaggtttac cttgatggcg cgaacatgaa
    22141 cgcccaggtt ggcatcacct cgccgggctt tattggcgcg gacgtttcgc acctcaacct
    22201 gcataaaact ttctgcattc cgcacggcgg tggtggtccg ggtatgggac cgatcggtgt
    22261 gaaagcgcat ctggcaccgt ttgtaccggg tcatagcgtg gtgcaaatcg aaggcatgtt
    22321 aacccgtcag ggcgcagttt ctgcggcacc gttcggtagc gcctctatcc tgccaatcag
    22381 ctggatgtac atccgcatga tgggcgcaga agggctgaaa aaagcaagcc aggtggcaat
    22441 cctcaacgcc aactatattg ccagccgcct gcaggatgcc ttcccggtgc tgtataccgg
    22501 tcgcgacggt cgcgtggcgc acgaatgtat tctcgatatt cgcccgctga aagaagaaac
    22561 cggcatcagc gagctggata ttgccaagcg cctgatcgac tacggtttcc acgcgccgac
    22621 gatgtcgttc ccggtggcgg gtacgctgat ggttgaaccg actgaatctg aaagcaaagt
    22681 ggaactggat cgctttatcg acgcgatgct ggctatccgc gcagaaatcg accaggttaa
    22741 agccggtgtc tggccgctgg aagataaccc gctggtgaac gcgccgcaca ttcagaacga
    22801 actggtcgcc gagtgggcgc atccgtacag ccgtgaagtt gcagtattcc cggcaggtgt
    22861 ggcagacaaa tactggccga cggtgaaacg tctggatgat gtttacggcg accgtaacct
    22921 gttctgctcc tgcgtaccga ttagcgaata ccagtaattc actgattcga ctatcttcta
    22981 aaggcgcttc ggcgcctttt tagtcagatg acaaagtaca aaagcgctca gacagtcccc
    23041 tcaccccttt ggggagaggg ttagggtgag gggaacaggc cggcactggc gcgaatattt
    23101 accctcaccc cggccctctc cctgaaaggg cgaggggaaa agcgtgccaa cattgaagat
    23161 tgagccagtt tgttagcaat ctcaaagata cgtcaacgaa ttaatttttc tcggaaaaac
    23221 aaatggctat agcacttgtg accggtggca gtcgcggcat cgggcgggca actgcattac
    23281 tgttggcgaa agaagagtat acggtggcgg ttaattatca acaaaacctc catgcggcgc
    23341 aggaagtggt gaacttaatc acgcaagccg gtggcaaggc attcgtgctc caggcggata
    23401 tcagcgacga aaatcaggtc atcgcaatgt ttacagcaat cgatcagcac gatgaaccgc
    23461 tagcagcgct ggtcaataac gccgggatct tgtttaccca gtgcaccgtt gaaaacctta
    23521 ccgcagagcg aatcaaccga gtactttcca ccaacgtgac gggatatttt ctctgctgcc
    23581 gcgaggcggt aaaacgcatg gcgcttaaaa atggtggcag tggcggcgct atcgtcaatg
    23641 tctcttcggt ggcctcacgg ttgggttcgc caggggaata tgttgattac gcggcatcga
    23701 aaggggcgat tgatacgtta accaccggac tatcgctgga agtcgccgcg caggggatcc
    23761 gcgttaactg cgtgcggcca gggtttattt ataccgaaat gcacgccagc ggcggcgagc
    23821 ctggacgcgt cgatcgcgtt aagtcgaaca tccccatgca gcgtggtgga caggcagaag
    23881 aggtcgcgca ggccattgtc tggctactaa gtgataaagc ctcttacgtc acgggaagtt
    23941 ttatcgattt ggcgggcggg aaataaaaca gggaagttgt ctgaccggat gcaacaagta
    24001 ttgcatccgg tacttcatcg acttaaagct tctcgccgtt gctggcaatc acctctttgt
    24061 accagttaaa gctcttctta cgtgaacgcg acatatcgcc agtaccgtcg tcatgtttat
    24121 tcacatagat aaagccgtag cgtttgctgt actgcccggt agtgaatgac acgcagtcga
    24181 tgcagcccca cggtgtgtag cccatcagat ccacaccatc gtaagtcact gcttttttca
    24241 tctcttcaat atgggcgcgc aggtagtcaa tgcggtagtc gtcgttgatg ctgccatctt
    24301 cttccacttt gtcgtaagcg ccaaaaccgt tttcgacaat aaacagcggc ctctgataac
    24361 gctcatacag ttcgcaaagg gcatagcgca gacctactgg atcaatctgc cagccccagt
    24421 cagacgcttt aacatacggg ttcggtacgc tgccttcaaa accagagatc gcatcgcctg
    24481 tgccgccttc ggccttcact gcgttggtca tgtaatagct gaaaccaaga taatcgcagg
    24541 tgccttcacg cagtacatcg agatcgccgt cttccatttt gatgttaaat ccgcgacgct
    24601 cccactcgtt caacacatag gacgggtaat agccgcgtag ctgcacatcg gtaaagacgt
    24661 agcgttcgcg catcgactcc tgagcgaaca tcacatcgtc cgggttacag cagtagggat
    24721 agagcggcac catcgccagc atacagccga ctttcatctc cgggttaatg cgacgcgcag
    24781 ctttcaccgc cagggcgctg gcgacaaact ggtgatgcag cacctgatac atcgtctctt
    24841 ccgggttctc atgctcggta taaaccacgc cggagcagca gtaaccgaac agcggtgcac
    24901 gccagttacg ctggttgtta atttcgttga aggtcatcca gtatttgact ttatgcttat
    24961 agcgttcaaa caccacttcc gcgaaacgga caaagaaatc aaccacttta cggttggtcc
    25021 agctaccgta ttgctgcacc agatgcagcg gcatttcaaa gtgggagagg gtgatcaccg
    25081 gttcgatgtt gtatttcagc agttcatcga acatatcatc gtagaacttc agcccttctt
    25141 cgtttggctg agcttcatca ccttttggaa aaatacgcgt ccaggcgatg gaagtccgaa
    25201 aacatttgaa gcccatttcg gcaaatagct tgatgtcttc cttgtagtga ccataaaaat
    25261 caacggcttc atggtttgga tagtatttgc ctggcaagac ttctttggtg atttcgcgcg
    25321 gcacgccgtg tgcgccaccg gtcagaacgt cacaaatgct cggccctttg ccgcctttgt
    25381 tccagccgcc ttcgacctga tgagcggcaa ctgcgccgcc ccataagaaa tctttcggta
    25441 aggtgagttt tttcactatc ataggctcca gatgagtcaa ttcttacagg aaatagtacc
    25501 atgcagccaa acgacatcac tttttttcaa cgtttccagg atgacattct ggctgggcgt
    25561 aaaaccatca ccatccgcga cgagtctgaa tcgcacttca aaacgggtga tgtgcttcgt
    25621 gtcgggcgtt ttgaagatga cggttatttt tgcacgattg aagtcaccgc aacctcaacc
    25681 gtaacgctgg atacgctgac ggaaaaacat gcagagcagg aaaatatgac cctgactgaa
    25741 ctgataaagg tcattgccga catctatccc ggtcagacac agttttatgt gattgaattt
    25801 aaatgtcttt aaattgaact cacgcctgtt agttgaaatt tcgcatttaa tttcatgatt
    25861 tttaggagat tagtctcaaa tcagaattta ttttagctaa caggtgttca ctggaactat
    25921 tctcagttac gctggagagg tatacatgtg cgtgtacgtt tctccggagt aagttatggt
    25981 tcagaagccc ctcattaagc agggatattc gctggcagag gaaattgcca acagcgtcag
    26041 tcacggcatt gggttggtgt ttggtatcgt tgggctggtg ttgctactgg ttcaggcggt
    26101 ggatctcaat gccagcgcca cagcgataac cagttacagc ctctatggcg gcagtatgat
    26161 cctgctgttc ctcgcttcga cgctctatca cgccattcct catcaacggg caaaaatgtg
    26221 gctgaagaaa tttgaccatt gcgctattta cctgttgatt gccggaacct acacgccgtt
    26281 tttgctggtg gggctggatt ctccgttagc gcgcgggttg atgattgtta tctggagcct
    26341 ggcattgctg ggtattctgt ttaaactgac catcgcgcac cgattcaaaa ttttatctct
    26401 ggtgacctat ctggcgatgg gctggctgtc gctggtggta atttatgaaa tggcagttaa
    26461 gctcgcggcg ggcagcgtta ccttactggc ggtaggcggc gtggtttatt cgctcggggt
    26521 gattttctac gtctgcaaac gcattccata caaccatgcc atctggcacg gcttcgtgct
    26581 cggcggtagt gtgtgccact ttctggcgat ctatttgtat attgggcagg cgtaatgggt
    26641 atgc
//
LOCUS       DAMZET010000048        26533 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.048, whole genome shotgun sequence.
ACCESSION   DAMZET010000048 DAMZET010000000
VERSION     DAMZET010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.048"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..477)
                     /locus_tag="POB34_002100"
     CDS             complement(<1..477)
                     /locus_tag="POB34_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI5919047.1"
                     /translation="MINQLTFTKHYDTFDNISKIYSDKFPQGKDLDLLHIVLYFRFLS
                     YQENNLNCYESHETLAKIFKSSPSTIKRKINDLKEMGLLETSPHPDPYISSLIYNALP
                     LTDAHITPPGESSLSDLSEAEEAQEQPKGHKQPSFDVLDDDWDTPLPWETEETPVSS"
     gene            complement(531..701)
                     /locus_tag="POB34_002101"
     CDS             complement(531..701)
                     /locus_tag="POB34_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919048.1"
                     /translation="MSETTEKKRQPRTCFHIPDEVFQVLFKEARSRRITVTDYILDML
                     KREAETLSNKSK"
     gene            complement(721..909)
                     /locus_tag="POB34_002102"
     CDS             complement(721..909)
                     /locus_tag="POB34_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919049.1"
                     /translation="MKWFTPEHVVKAFKKGELTRHQIVMNRNMARSRGYPERAACFNE
                     ALKIIDELRKNEKESETE"
     gene            2879..3418
                     /locus_tag="POB34_002103"
     CDS             2879..3418
                     /locus_tag="POB34_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI5919050.1"
                     /translation="MSNIGYKRVSTVDQNTDRQLAGVTLDKVFEDKLSGKDTNRPGLQ
                     ACLAYLREGDTLHVHSLDRLGRNTLDILALVEQLNNRGVIVRFHKEGIIADITSAMGK
                     MMLTMLSAVATCERELMLERKREAQAVNPTKRGNGKAVDREGIKKALSDGLSVRKTAE
                     SFNVAPSTVQRIKKEEEEA"
     gene            complement(3970..4122)
                     /locus_tag="POB34_002104"
     CDS             complement(3970..4122)
                     /locus_tag="POB34_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919051.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLKD"
     gene            complement(4728..4803)
                     /locus_tag="POB34_002105"
     tRNA            complement(4728..4803)
                     /locus_tag="POB34_002105"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4768..4770),aa:Thr,seq:cgt)
     gene            complement(4918..6171)
                     /gene="proA"
                     /locus_tag="POB34_002106"
     CDS             complement(4918..6171)
                     /gene="proA"
                     /locus_tag="POB34_002106"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDI5919052.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPVGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MEESGVALHADAAALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(6183..7286)
                     /gene="proB"
                     /locus_tag="POB34_002107"
     CDS             complement(6183..7286)
                     /gene="proB"
                     /locus_tag="POB34_002107"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDI5919053.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7574..8629
                     /gene="phoE"
                     /locus_tag="POB34_002108"
     CDS             7574..8629
                     /gene="phoE"
                     /locus_tag="POB34_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HDI5919054.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(8668..9069)
                     /gene="crl"
                     /locus_tag="POB34_002109"
     CDS             complement(8668..9069)
                     /gene="crl"
                     /locus_tag="POB34_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDI5919055.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(9127..10371)
                     /gene="frsA"
                     /locus_tag="POB34_002110"
     CDS             complement(9127..10371)
                     /gene="frsA"
                     /locus_tag="POB34_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDI5919056.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(10463..10921)
                     /gene="gpt"
                     /locus_tag="POB34_002111"
     CDS             complement(10463..10921)
                     /gene="gpt"
                     /locus_tag="POB34_002111"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDI5919057.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDNYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            11182..12639
                     /gene="pepD"
                     /locus_tag="POB34_002112"
     CDS             11182..12639
                     /gene="pepD"
                     /locus_tag="POB34_002112"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDI5919058.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQDILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIKSVGHYWTLLTELLKEIPAK"
     gene            complement(12696..13048)
                     /gene="prfH"
                     /locus_tag="POB34_002113"
                     /pseudo
     CDS             complement(12696..13048)
                     /gene="prfH"
                     /locus_tag="POB34_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(13044..13250)
                     /locus_tag="POB34_002114"
                     /pseudo
     CDS             complement(13044..13250)
                     /locus_tag="POB34_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(13569..14021)
                     /locus_tag="POB34_002115"
     CDS             complement(13569..14021)
                     /locus_tag="POB34_002115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5919059.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVMMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFISRIERHIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQIVKQQHVECRGAWFTNFYMRYKPQH"
     gene            complement(14018..15073)
                     /gene="dinB"
                     /locus_tag="POB34_002116"
     CDS             complement(14018..15073)
                     /gene="dinB"
                     /locus_tag="POB34_002116"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDI5919060.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELHLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(15144..15929)
                     /gene="lafU"
                     /locus_tag="POB34_002117"
     CDS             complement(15144..15929)
                     /gene="lafU"
                     /locus_tag="POB34_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HDI5919061.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFSGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            15901..17613
                     /locus_tag="POB34_002118"
                     /pseudo
     CDS             15901..17613
                     /locus_tag="POB34_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(17931..18428)
                     /gene="rayT"
                     /locus_tag="POB34_002119"
     CDS             complement(17931..18428)
                     /gene="rayT"
                     /locus_tag="POB34_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HDI5919062.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRHAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(18604..19353)
                     /locus_tag="POB34_002120"
     CDS             complement(18604..19353)
                     /locus_tag="POB34_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDI5919063.1"
                     /translation="MSSFLLGRFLHPGVFSLCVLLPLFASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GENIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            19654..20394
                     /locus_tag="POB34_002121"
     CDS             19654..20394
                     /locus_tag="POB34_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HDI5919064.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(20365..21132)
                     /locus_tag="POB34_002122"
     CDS             complement(20365..21132)
                     /locus_tag="POB34_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HDI5919065.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(21338..21916)
                     /gene="lpcA"
                     /locus_tag="POB34_002123"
     CDS             complement(21338..21916)
                     /gene="lpcA"
                     /locus_tag="POB34_002123"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HDI5919066.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            22156..24600
                     /gene="fadE"
                     /locus_tag="POB34_002124"
     CDS             22156..24600
                     /gene="fadE"
                     /locus_tag="POB34_002124"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HDI5919067.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPFDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYLGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLCSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(24643..25089)
                     /gene="ivy"
                     /locus_tag="POB34_002125"
     CDS             complement(24643..25089)
                     /gene="ivy"
                     /locus_tag="POB34_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HDI5919068.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            25270..26040
                     /gene="yafV"
                     /locus_tag="POB34_002126"
     CDS             25270..26040
                     /gene="yafV"
                     /locus_tag="POB34_002126"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HDI5919069.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQNDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMVALREYREKFPAWQDADEFRLR"
     gene            complement(26082..>26533)
                     /locus_tag="POB34_002127"
     CDS             complement(26082..>26533)
                     /locus_tag="POB34_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HDI5919070.1"
                     /translation="HGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQ
                     KKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAE
                     LFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAESGLS"
BASE COUNT         6643 a         6593 c         6533 g         6764 t
ORIGIN      
        1 tgatgaaaca ggtgtttctt ccgtctccca cggtaaaggc gtatcccaat catcatcaag
       61 aacatcaaaa gaaggctgtt tatggccttt aggttgttcc tgtgcttctt ctgcctcaga
      121 aagatcagag agtgatgatt ctcctggggg agtaatatgt gcatctgtga gagggagagc
      181 attgtaaatc aaagatgaaa tatacggatc aggatgtggg gatgtttcta ataatcccat
      241 ctctttaaga tcgtttatct tacgtttaat tgttgatggt gaagacttaa aaatcttagc
      301 taatgtttcg tgactttcat aacaattaag gttgttttcc tgataactaa ggaatcgaaa
      361 ataaagcaca atatgtaata gatctaaatc ttttccttga gggaatttat cagaataaat
      421 ctttgatata ttatcaaacg tgtcatagtg ttttgtaaaa gttagttggt taatcattaa
      481 aaatctcctt gatgtaatca cgctctatgg cgctactatg gtgataaaaa tcacttgctt
      541 ttatttgaga gtgtttcagc ctctcttttc aacatatcaa gaatgtaatc tgtgacagtg
      601 atccgtctac ttctggcttc tttaaataac acctgaaata cttcatcagg aatatgaaag
      661 caagtgcgtg gttgtctttt cttttctgta gtttcgctca tagttttctc cttctcttct
      721 ttactctgtt tcagattctt tttcattttt tcttagttca tcaataatct tgagtgcttc
      781 attgaagcaa gctgctcgct ctggataacc tcgactcctt gccatattac gattcattac
      841 tatttggtgg cgtgttagct cacctttttt aaatgccttt acgacgtgct ccggtgtaaa
      901 ccatttcata aaaaactcct tattcagtta aaagaaataa aacaccttgc aagcctgacg
      961 gcttcaaagt gcattgattt tgattattta gagaagaatg tttttctgtc cctctaatac
     1021 ctattattac atataaagaa acatttgtca ataccctttc cgaaaaaaat cgatcttttt
     1081 catgcactta cgagcaactc gtgctcttct gtgtagtgct attcttcatt atccgctaaa
     1141 acgctctaaa acgcgttcta acgaagtttt gataaaagca atacattcct tatgtaatgg
     1201 ctgtttagct cgccagaggc ttatttaagg ggcttatttt gtatttgtta ggtatatgaa
     1261 aaggtaatgg taaatataaa acagagagtc actttataaa cgctctgtaa agccctgtag
     1321 tgagtttata tatgtaggca aagcggtttc cattctctac cctgcttaat gctgttagag
     1381 aagcatttag accccttatt tttaattaat taaaatatcc tctatacatt cctgttatga
     1441 aacacctctg aatccttcat aaaacacatt atgacgcaaa gaacactttt ctaaggtgat
     1501 tccctttctg aatgcggttt cttgcgtcag agagctacac agagcttata aagctattat
     1561 taataataaa gtagataaag taagtaagat aaagtaagaa gattaactaa gatagataag
     1621 attaataaga tcagaattct ctttcggtgt cgcctctggc ttttccaccg aaaacaatta
     1681 cacgactaca gaagtaggaa gtggaattgt attgaggctt agtgaaatta cacgaggaaa
     1741 gcagagtgta attgagcttt agccgctctc tcttcgagta attacacgac tacagagaat
     1801 aacagagtta ttaaatgtag gagggtaatt aagagttaga gagagttaac acgtaagcaa
     1861 tcagtatgat tggttaggtg attactcttc tgcgcacagc gcaatagtat ttcttcgtgg
     1921 gcgttaccgc cacgatagtt acacgaagaa agtaacgctt tgctttcggt gtgagcggaa
     1981 cgggccagcc aacgtagccc aaaggtagcg gagtaaggct gcttgccaga gagtgaagct
     2041 aacgccggaa ggcaaaagat catatatata tatagatata aagaatatta ttattatgat
     2101 ctaattaata atctaatata gttttattaa tagtcttatt gtagttcaca gtgacctacc
     2161 ccctagctca ctatgaccta ccccctagct cactatgacc taccccctag ctcactatga
     2221 cctaccgtta ctaccaactt tcttacagag agcatttcag atagctgaat gtaacctctc
     2281 gttacatcca tttgtcacat agcactttgt gtaatgtctt tctatgtgtg tgccataatc
     2341 cccctatatt ccctgtgtaa atcatagaat atccctaact tattcctgtg taattccata
     2401 ctaaaaattc aatgaaaaag tagcttaaaa gtgcaattct tactttcttc acttttacat
     2461 cactttcgca aaaccttgtg tagtacggct tacagagcac catcagaaat tgttggctaa
     2521 aaattgttct gcttgtttct taacgagttt tctaatttgt gcaaatttta aaatttattt
     2581 tgtttttaca gccttatttc gtgtaatgcg tggcatagca agcccttttc ttgtgttgtc
     2641 aatacatttg gcaacgttca aacatctgtt tagaaatgcc aaattgttga tctaacaaat
     2701 aaacctgtat aaacagaaca ccatttaatc tattcccctg tgtatccatt aggcatacca
     2761 aatgaataca gctaaatcgt gtaatattcc gtacccataa ggcttgaaac agaccaaatg
     2821 aatacggtat actcatttgc attgaagtag tgccaaatcg acacattaag aggattttat
     2881 gagcaatatc ggttataagc gtgtgagcac tgtagatcag aacactgatc gccagcttgc
     2941 aggagtaact ctcgataaag tctttgaaga taagctgtct ggtaaagaca ctaaccgccc
     3001 aggcttacaa gcgtgtttag cctatctgag agaaggcgac accttacacg tgcattctct
     3061 cgaccgttta ggacgtaaca ccttagacat ccttgcttta gttgaacaac ttaataatcg
     3121 aggtgtaatt gttcgatttc acaaagaagg aatcattgca gacattacaa gcgcgatggg
     3181 aaagatgatg cttacgatgt tgtcggcagt tgcaacgtgt gaacgtgaac tgatgttaga
     3241 acgtaaacga gaagcgcaag ccgtcaatcc tacaaaacgt ggtaatggaa aagcagtgga
     3301 tcgagaaggt ataaagaaag ccctttctga tggcctgtca gtgcgtaaaa ccgccgagag
     3361 ctttaatgtt gctccttcaa cggtgcaacg tatcaagaag gaagaagagg aggcgtaatg
     3421 cctccttgat atttgagaat tgtcgctgcg tggacctgac gccgctgatc ttcgcgttaa
     3481 acattgatca cgtctgcgcc gatttcagat aaaaaggtag ctattaagca atacgtaact
     3541 tttacaatta gtgaaattat ctaaaacaca agttatatag atgagaaaat gaaacattat
     3601 cttgaacaaa tcaaacaaaa caacacatca tcttgttgac accatacaaa gtgaatagta
     3661 aactattata tgtgaaaatt tgcatgttgc gtgcaataaa aaataaatga tactttgcta
     3721 ttgttaaatc aatagggggt atctatgcgc tttatctcat ccattttaaa aacctgcaga
     3781 accacaatta ctttagctgt aattgttacc atcatatacc tcatctattt tacccctcga
     3841 gataaaatcg attcactagc gatcatcgtt catgcaatgg ctacattaac taaaaaactt
     3901 taataatttc actatcaggc gcataaacac atctttgatc gctggatgtg ttatgcgcct
     3961 actggtgatt caatccttaa gccatttcaa ctcactaagg agttttcttt cttcgttctc
     4021 tctctgttta cgctctagcc aggcttttcc ctctggcgta gacaggaact tacgagcgtg
     4081 aatcttgcgg ttattacgtt tgatagctgc aatgtttttc atcgtgtgcc tctgtattca
     4141 tatgaaaatt aacaagttac atactacaca gggagactac gcataaaaca caatacaagt
     4201 aaagatacat tttcaagaca ataggagcca taaagcgcct ttgatggtta taccaaaaaa
     4261 taagcgccgt aggcgtatcc actaaaaagg gctttttcac aaaagctatg aagtcttagc
     4321 agttcttctc ttatcaggca cccgttgtac tccggtggac gaaaccgtgc tagagcttga
     4381 ttctttcccg atcaagttag gttttgaaga ggtgggcttt gatccactat tgcacttaaa
     4441 actcttccca tcatttagcc tgtttatcct ctgcaacgtc tcacgacgtg tagcatcatt
     4501 ggacaccgcg ccaggtgtgg aacatcttgc taagagtttt ggtagaaggt ttagagacgg
     4561 tggcacgtct tccagcttca aaggaacttg ttacgaggtt gagcctggga actactcgcc
     4621 catacgtgtt acactaccaa agcccataac aacgtatagg cgtttgtaag tgataacaga
     4681 agatacaatt tcttagagaa gttaagctaa gttattgatt ttcttgatgg tgccgataat
     4741 aggagtcgaa cctacgacct tcgcattacg aatgcgctgc tctaccaact gagctatatc
     4801 ggccctgaaa ggacatgttc acgaacgtga atcacggtgg acaaggttaa aactaaccgg
     4861 gcgatgcgtc aatggccttg tgaatcaaat ggctactttt gcatcacccg gttttattta
     4921 cgcacgaatg gtgtaatcac caataccgat ccacttgtaa gtggtcagtg cttccagccc
     4981 cattgggcca cgcgcgtgga gtttttgtgt gcttaccgcc acttccgcac ctagtccaaa
     5041 ctggccgccg tcggtaaaac gcgtagaggc gttaacgtaa acagcggacg aatccacttc
     5101 gttaacaaaa cgctgggcgt tgcgcatatc gcgggtcagg atcgcatcgg agtgttgtgt
     5161 gccgtgttca cgaatatggg cgatggcatc gtcaagatca ctgacgattt tgacgttcaa
     5221 atctaatgac agaaactcat cgtcatactc ttccgcttta acagccacca ccttcgcggg
     5281 gcctgtctgc aactgcgcca gcgcagctgc atctgcgtgt aatgccacgc cgctttcctc
     5341 catttgtttg cttaatgcgg gcaggaagct atcggcgatg tttttattca ccagcaacgt
     5401 ttctaccgta ttacatgtgc tcggacgctg agttttcgcg ttgacgatca cttttaatgc
     5461 ttcagcaatc tctacacttt catcaacata aatatggcat acgcctatac cacctgtgat
     5521 caccgggatc gtcgactgtt cgcggcacag tttatgcaaa ccagcgccac cacgcgggat
     5581 cagcatgtcg atgtatttat ccatacgcag catttcactg accagcgcac ggtcaggatt
     5641 atcaatcgcc tgcacggcac ccaccggtaa gccacaggat ttcagggcgt cctgaatcac
     5701 cgccaccgtt gccgcgttag tgcgacaggt ttctttaccg ccacgcagaa tcactgcgtt
     5761 accggttttc aggcacagcg aagcgacatc aaccgtcacg ttcgggcgcg cttcataaat
     5821 cacgccaata acccccagcg gtacgcgacg acgctcaaga cgcaggccgc tgtccagtac
     5881 gctgccatcg attacctgcc ccaccggatc ggcgaggtta cacacctggc gcacatcatc
     5941 ggcaatgcct ttcagccgtg cgggcgtcag tgccagacgg tcaagcatcg cttcgccaag
     6001 gccattggca cgcgcgtcag caacatcctg ggcgttagcg ttgaggatga tttcgctttg
     6061 tgcttccagt tcatcggcga ttttttccag cacgcgattt ttttcgcggc tggagagttg
     6121 cgctaattta tacgaggctt gcttcgcggc aatgcccatt tgttccagca tcagcctgct
     6181 ccttaacggg taatcatgtc atcacggtga acggcaaccg ggccgtattc atatcccagt
     6241 attgcatcaa tttcttgcga gtggtgcccg gcaatacggc gtaatgcatc gctgttgtaa
     6301 cgactgacgc cgtgggcgat atcgcgacct tcgaggttgc aaatgcggat gacttcacca
     6361 cgcgagaaat tgccagtcac gcttttaatg cctttcggca acagggagct gccgcgttca
     6421 agaatggcgg cagttgcccc ttcatctacc gtgatttcac ccgccggcgg cgcaccgaaa
     6481 atccagcgtt tacggttttc aagcggagtc gcctgggcat ggaacagcgt accgacggaa
     6541 atgccttcca tcacatcacc aataacgccc ggcttgctgc ccgcggcaat aatggtgtcg
     6601 atacccgcac ggcaagccac gtcagcggcc tgcaatttgg tactcatgcc gccagttccg
     6661 aggcctgaaa cgctgtcacc ggcaatcgcg cgcagtgcgt catcaatgcc gtaaacatct
     6721 ttaatcagtt ctgcctgcgg attgctgcgc ggatcagcgg tatacaaacc tttttgatcg
     6781 gtcagcagca acagtttatc ggcacccgcc agaatcgccg ccagcgcaga aaggttatcg
     6841 ttatcgccga ccttaatctc tgccgtagcg acagcatcgt tctcattgat taccggaacg
     6901 atattgttat cgagcaacgc acgcagggtg tcgcgggcgt tcaggaagcg ttcacggtct
     6961 tccatatcag cacgggtcag cagcatctgc ccgacgtgaa tgccataaat cgaaaacagc
     7021 tgttcccaca gttgaatcag tcgactctgc cctaccgccg ccagcagttg tttcgaggcg
     7081 atagtcgctg gcagttccgg gtaccccagg tgctcacgtc cggcggcgat cgcgcccgac
     7141 gtcacaataa caatccgatg cccggcggca tgtaactgcg cgcactggcg aacaagttca
     7201 acgatatggg cacggttcag acggcgcgat ccgcctgtta gcacactggt gccgagtttt
     7261 accaccagcg tctggctgtc actcatgatt ctctgccatt caattttagg aaaaatgata
     7321 tcaaacgaac gttttagcag gactgtcgtc ggttgccaac catctgcaag caaagcatgg
     7381 cgttttgttg cgcgggatca gcaagcctag cggcagttgt ttacgctttt attacagatt
     7441 taataaatta ccacatttta agaatattat taatctgtaa tatatcttta acaatctcag
     7501 gttaaaaact ttcctgtttt caacggggct ctcccgctga atattcgcgc gttaattaaa
     7561 atcaggaatg aaaatgaaaa agagcactct ggcattagtg gtgatgggca ttgtggcatc
     7621 tgcatccgta caggccgcag aaatatataa caaagacggt aataaactgg atgtctatgg
     7681 caaagttaaa gccatgcatt atatgagtga taacgacagt aaagatggcg accagagtta
     7741 tatccgtttt ggttttaaag gcgaaacaca aattaacgat caactgactg gctatggccg
     7801 ttgggaagcg gagtttgccg gaaataaagc ggagagtgat actgcacagc aaaaaacgcg
     7861 tctcgctttt gccggattga agtataaaga tttgggttct ttcgactatg gccgtaacct
     7921 gggcgcgttg tatgacgtgg aagcctggac cgatatgttc ccggaatttg gtggcgactc
     7981 ctcggcgcag accgacaact ttatgaccaa acgcgccagc ggtctggcga cgtatcggaa
     8041 caccgacttc ttcggcgtta tcgatggcct gaacttaacc ctgcaatatc aagggaaaaa
     8101 cgaaaaccgc gacgttaaaa agcaaaacgg cgatggcttc ggcacgtcat tgacatatga
     8161 ctttggcggc agcgatttcg ccattagtgg tgcctatacc aactcagatc gcaccaacga
     8221 gcagaacctg caaagccgtg gcactggcaa gcgtgcagaa gcatgggcaa caggtctgaa
     8281 atacgatgcc aataatattt atctggcaac tttttattct gaaacacgca aaatgacgcc
     8341 aataactggc ggctttgcca ataagacaca gaactttgaa gcggtcgctc aataccagtt
     8401 tgactttggt ctgcgtccat cgctgggtta tgtcttatcg aaagggaaag atattgaagg
     8461 tatcggtgat gaagatctgg tcaattatat cgatgtcggg gctacatatt atttcaacaa
     8521 aaatatgtca gcgtttgttg attataaaat caaccaactg gatagcgata acaaattgaa
     8581 tattaataat gatgatattg tcgcggttgg catgacctat cagttttaat gattattgcc
     8641 ggatgtgatg catccggcac atttcactca cgccgttaac ttcaccggct cgtcacgaaa
     8701 atcatccgcc ggttccagct tcagattcag cgtcgtcagc agttcacgca gcttctcgtg
     8761 aaactcacgc agggtgtgct ccagtcgttc aaccacttca gtgtctttta ccggaacgct
     8821 cttccagtcg cctgctttat cgaacagacc aaactggtag ctgtaggtaa aacgggattc
     8881 ctgcgcttcc agctccatcc accagcccca gaattcacgc acttccggtg ccggtttcac
     8941 gttgacgcat acagccagac aatcgaaaaa gaatcgatta tcttcgcact taccttcacg
     9001 aatatacggg cctagtgcgg taaatttttt gatcaatctg ctcttcgggt gtccactcgg
     9061 taacgtcatt gctatctcct gttgtgatgc aactgtttta ccaaattggc aaaatttagc
     9121 aaatttttaa cacaagcgtt tttcgatcca gccggtgatt tcctgaagac ctttgtcaaa
     9181 attccgatac accgggttaa atgggatctc taataattta ccgtcagcag atgatgaggt
     9241 gattaagcgt gagtcctctt ccgggctgaa cggatcgttc ttccagtagc ctgataacat
     9301 tggcgttggg cagcgacgtc ccagcaatcc ttgcactttt aatgaatagc gattcagctc
     9361 cacgcgcaac gcgtcatcgg aagcatcatg catccccaaa cgactcgcca gaacgtcaag
     9421 atacatttcc ggcacctgtt gctggcactt aaaatcactc aacagggtat gaactaccgg
     9481 accaagacag gcaaccgctt tcagacgcgg cgattcaagg tatgccagac gcacggcaac
     9541 gttagcgccg aaacggaaac caaaggccgc gacgcgagtg tgatccaccc acggtacgtt
     9601 aggcagcgcc tttaagacgt gctgatgcaa caggctggag tcctgggtga gcttccattt
     9661 tgaagaaaag cccaccgacg gcatatcaat agtcagcatc gcaatgccgc gcggcgcaaa
     9721 ataacgttca tacaggctgt aatagtccgt ctgcatcgca tccagaccac cacacattaa
     9781 tactgtcggg aacgggccat cgcctttcgg catatgcaaa aagccggtga tgggcgcacc
     9841 gccgggtacg gtaaactcca tctgccgcat cgtgcccggc agacgctggg cggcctcttc
     9901 ataggcgcgg tttgataaag cctgcgcttg ctcggccagg tcatctcctt tcagatgagg
     9961 ataggcggca atgttgtaca acgtagccgc atgcagccag tgtcgaccac tgagttgcgg
    10021 atcttcttcc gcacaggctt tttgttgcca caccatcgcc tgggtggccc actcataaat
    10081 ccagttgcca ccacggtagc caatcacggt atcgtataaa tcatcatcgg tacgttcagc
    10141 atcgctcatc acaatacgtg cctggacgtc gaggatttca cgtgggtcaa tgccgcgcca
    10201 gatccacatc agacggttaa tcatgcgata ccagtgaggg atggttttac catcaagggc
    10261 cgactgcaca ggcggttgtg cgccgtgatt aaagcggcgg actagcgtcg aggtttcagg
    10321 atgtttaaag cggggtttga acagggtttc gctcaggttt gcctgtgtca tggatgcagc
    10381 ctccagaata cttactggaa actattgtaa cccgcctgaa gttaaaaaga acaacgcccg
    10441 gcagtgccag gcgttgaaaa gattagcgac cggagattgg cgggacgaat acgacgccca
    10501 tatcccacgg ctgttcgatc caggtatctt gcgggatatc aacaacataa ttatcaacca
    10561 gcggacgacc agccggtttt gcaaagatgg tgacaaagtg cgcttttgga tacatttcac
    10621 gaatcgcaac cgcagtacca ccggtatcca ccaggtcatc aataacgatg aagccttcgc
    10681 catcgccttc tgcgcgtttc agcactttaa gctcgcgctg gttgtcgtga tcgtagctgg
    10741 aaatacaaac ggtatcgaca tgacgaatac ccagttcacg cgccagtaac gcacccggta
    10801 ccagaccgcc acggcttacg gcaataatgc ctttccattg ttcagaaggc atcagtcggc
    10861 ttgcgagttt acgtgcatgg atctgcaaca tgtcccaggt gacgatgtat ttttcgctca
    10921 tgtgaagtgt cccagcctgt ttatctacgg cttaaaaagt gttcgagggg aaaataggtt
    10981 gcgcgagatt atagagatct ggcgcactaa aaaccagtat ttcacatgag tccgcgtctt
    11041 tttacgcact gcctctccct gacgcgggat aaagtggtat tctcaaacat atctcgcaag
    11101 cctgtcttgt gttgacaaca ttttctgcta accctgtgac ctgcaatact gttttgcggg
    11161 tgatcgacaa ggagacttaa cgtgtctgaa ctgtctcaat tatctccaca gccgctgtgg
    11221 gatatttttg ccaaaatctg ttctattcct cacccgtcct atcatgaaga gcaactcgct
    11281 gaatacattg ttggttgggc aaaagagaaa ggtttccatg tcgaacgcga tcaggtaggt
    11341 aatatcctga ttcgtaaacc tgctaccgca ggtatggaaa atcgtaaacc ggtcgtcttg
    11401 caggcccacc tcgatatggt gccgcagaaa aataacgaca ccgtgcatga cttcacgaaa
    11461 gatcctatcc agccttatat tgatggcgaa tgggttaaag cgcgcggcac cacgctgggt
    11521 gcagataacg gcattggtat ggcgtctgcg ctggcggttc tggctgacga aaacgtggtt
    11581 cacggcccgc tggaagtgct gctgaccatg accgaagaag ccggtatgga cggtgcgttc
    11641 ggcttacaga gcaactggtt gcaggctgat attctgatta acaccgactc cgaagaagaa
    11701 ggtgaaatct acatgggttg cgcggggggt atcgacttca cctccaacct gcatttggat
    11761 cgtgaagcgg ttccagctgg ctttgaaacc ttcaagttaa ccttaaaagg tctaaaaggc
    11821 ggtcactccg gcggtgaaat ccacgttggc ctgggtaatg ccaacaaact gctggtgcgc
    11881 ttcctggcgg gtcatgcgga agagctggac ctgcgcctta tcgatttcaa cggtggcaca
    11941 ctgcgtaacg ccatcccgcg tgaagccttt gcgaccattg ctgtcgcagc tgataaagtc
    12001 gacgccctga aatctctggt gaatacctat caggacatcc tgaaaaacga gctggcagag
    12061 aaagagaaga atctggcctt gttgctggac tctgtagcga acgataaagc tgctctgatt
    12121 gcgaaatctc gcgatacctt tattcgtctg ctgaacgcca ccccgaacgg tgtgatccgc
    12181 aattccgacg tggcaaaagg tgtggtcgaa acctccctga acgtcggtgt ggtgaccatg
    12241 actgacaata acgtagaaat tcactgcctg atccgttcac tgatcgacag cggtaaagac
    12301 tacgtggtga gcatgctgga ttcgctgggt aaactggctg gcgcgaaaac cgaagcgaaa
    12361 ggcgcatatc ctggctggca gccggacgct aattctccgg tgatgcatct ggtacgtgaa
    12421 acctatcagc gcctgttcaa caagacgccg aacatccaga ttatccacgc gggcctggaa
    12481 tgtggtctgt ttaaaaaacc gtatccggaa atggacatgg tttctatcgg gccaactatc
    12541 accggtccac actctccgga tgagcaagtt cacatcaaaa gcgtaggtca ttactggaca
    12601 ctgctgactg aactgctgaa agaaattccg gcgaagtaat tatttgattt gctgccggat
    12661 ggcgtttaat cgccttccgg cagtttcatc cttcattatc cttcgataaa agccatccct
    12721 gtaaatgtcc gtcgcgggtt gccacgttca atctggtgat ggaacattcg ccgctgcgat
    12781 ttcagcgccg cgctattttc ctgttgctgc tgctccagct tccaggcaat cagcaatcgc
    12841 gccagccgtt tgttagcatg cccagaaacc agtttttgcg cccatgatga gaccgatacg
    12901 gactcggaca aatccactga atagtgccgc accagctttc gcttaatgcc catgcgttat
    12961 cgccatccag agaaaccagc gccgaacgca gcgtgtcaga gtagcggccc gtttctgttt
    13021 ccagcaccgt taccgcgacg tcttgccgga taaaaacgac caacagagaa gaacgctgcg
    13081 ccaatcggat agccgcgtcc gggatgtaaa tctggcatcc ccactacgcg ctgcatgttg
    13141 ggtaaatttg ccgtggtgtg taattgttgg atcgctaaac tctcgatcca caggtcatca
    13201 gatgcgatgg taaataccgc atcggataag ggacgaatat atttgcccat gtaccattcc
    13261 atttatactc gtcatacttc aagttgcatg tactgcgact gcgttcgttc accccagtca
    13321 cttactttag taagctcctg gcgattcact cgcttgtcac cttcctgcaa ctcgaattat
    13381 ttagagtata taaatattaa aaatcaggaa atggcagacg aatccagggc aagaaaaaaa
    13441 gaattaatcc tggaaaggtc tgcaaagagt cattgataaa ggatttgtca tgatttacct
    13501 ccttgcagtt tgtggaataa gaaaacgcgc acataccagg cgggcgttat tttcattgca
    13561 agctggattt aatgttgcgg tttatatcgc atataaaaat tagtaaacca cgctccccgg
    13621 cattcaacgt gctgctgctt aactatctga aaaccataac gttcaaaaaa gggttttgcg
    13681 gttatgctgg cgtccaccgt aagttcggat tcagacttaa tcaaaggttt taacaaggcg
    13741 ctggcaaccc cgcggcgggt gtattcaggg tcaacaaata acatatcgat atgacgttca
    13801 atgcgggaaa taaaaccaac cggttgtgca ttaatgactg caacccgcac ttgtgatttc
    13861 gcgagtttct ccttccagcg agattcgtca atctgcgccc aggcggcaat ttgttgtggt
    13921 gaataatgct gactggcggt catcataacc gctctaatga aaatagcgca tagttgctga
    13981 aaatcgccag gctgatagtt tcttatttgt atgttattca taatcccagc accagttgtc
    14041 tttccatttg agggtcaagc aacgtcacat gcagccccac cagtcgcaca ccgcgcccgc
    14101 cgcggcgttc atcccaggtt ttacgcgcgg tggcgattag atcagcttta ttcagccgcg
    14161 gccagacgtg ctcctgggtg gtttgctgaa aatcgtcgaa ctttaatttc accccctggc
    14221 gagcaatcag taaatcaggt tttacctttg ccagacggcg ttcaagttcc ggatacagcc
    14281 gctcgataat cgcttcacat tcagaccagt gatgaatatc ttccgccatc gtgcgttcca
    14341 caccgacgga ttttcgcaac cgttcgctgt taacgtcgcg ttcgtcaatc ccctgactac
    14401 gctcccacaa aatgcggcca aatttgccaa agcgtttaag cagaatcacc agatcacact
    14461 tttgcacatc accgcaggtc cgcagcccca tcgcttccag ttttgccgcc gagactttgc
    14521 cgacgccggg gatttttgcc aatggtaagg tttgcagaaa tgccggaact tctgccggag
    14581 taatcacaaa ctggccgttg ggtttattca tgtcggaggc gattttggcg agaaacttga
    14641 ccggcgcaac gcccgcagac gccgtcaggt gcagctcgtt gaaaatcgtc tggcggattt
    14701 cctgggcgat gagggtcgca gaaccgtggc aatggacgct atcggtgaca tcgagataag
    14761 cctcatccag tgacaacggt tcaatgcgcg aggtgtagcg cgagaagatt tcacggatat
    14821 gatttgaggc ttctttgtag gcgtcaaagc gccccggaag caaggtgaga tgtgggcata
    14881 atttgagcgc catcccagta ggcatagcgc tacgtacgcc aaatttacgc gcgggataat
    14941 tggcggtgct gatcaccccc cgacgttcgc ggctgccgcc aatagcaata gggatatcgc
    15001 gcagggcggg attgtcgcgc atctccactg cggcgaaaaa gcagtccata tccacatgaa
    15061 tgattttacg cattgctcac ctctcaacac tggtaaagta tacagtgatt tcagggtttg
    15121 agaaatgcgt aaagattcag catttagcgc attcgctgcg aaagcacctg ctgcttatcc
    15181 agcttttgca ccagcggctg caccacttta tccccatgct gaccaaagta ttgatacagc
    15241 gtatcggacg cacttttggt cagcaccata atctcaatac gccgattgcc cgcgctttgc
    15301 ggatttttgg catccagcag catctggtcc gccattgcgc ttacctgcat cactttatct
    15361 tccggcattc cggcctcttc cagcacccga cgagctgaaa gcgcgcgatc gcccgaaagg
    15421 ttccagttgt tgtagatatt gtttttgtag gccatcgcat cggtatgccc ggtaataata
    15481 attttgttat cgagcgagtc gaacactggc gcgagctcca ccagcaaagt tttaaagaac
    15541 ggcataatct gcgcgctgcc gcgttcgaac atattgcggt tctggtcatc tttaatcagc
    15601 acgcgcaatc cctggggaac aatctccatc tccagattcg cttccatatg cgcatcacgg
    15661 gcaatggtgt tgatactggt tgccagctcg ccaagctcgg tggcggattt cttcttcagc
    15721 agcgctgttt tctcgttgac gtctcgcgct tttttctccg tctcttccgg caccgccacg
    15781 gtggcaggtt ttggcggatg agacgggctg attttattta acggcgacaa tccgccgcca
    15841 ctgaaaatcg actgcccgtg cagcgcggcg ataatgcttt cacgctcgga tttactgacg
    15901 ctgttgacaa tcaactttat cgtcgtcacc aaaggggccg agcgtatttc cgaggtttct
    15961 gcccgcttta ccctggacgc gatgcccggc aaacagatgg cgattgacgc cgaccttaac
    16021 gccgggttga tcaaccaggc gcaggcgcaa acccggcgta aagatgtcgc cagcgaggcc
    16081 gatttctacg gcgcgatgga cggggcgtca aagtttgtgc gcggggatgc catcgccggg
    16141 atgatgattc tggcaatcaa cctgatcggc ggcgtctgta tcgggatctt caaatacaac
    16201 ctgagcgccg atgctgcctt ccagcagtat gtgctgatga ccatcggcga cgggctggtg
    16261 gcgcagatcc cttccctgct gctctccacc gcggcggcga ttattgtcac ccgcatcagc
    16321 gacaacggcg atatcaccca tgacgtgcgc caccagctgc tggcaagccc gtcggtgctc
    16381 tacaccgcta ccgggatcat gttcgtgctg gcagtggtgc cggggatgcc gcacctgccg
    16441 tttttgctgt tcagcgccct gcttggcttt accggctggc ggatgagcaa acgcccgcag
    16501 gcggcggagg cggaagaaaa aagcctcgaa acgctgaccc gcaccatcac cgaaaccagc
    16561 gagcaacagg tcagttggga gacgataccg ctgatcgagc ctatcagctt aagcctcggc
    16621 tacaagctgg tggcgctggt ggataaagcg cagggcaacc cgctcaccca gcggattcgc
    16681 ggcgtgcggc aggtgatatc cgacggtaac ggcgtgctgc tgccggagat ccgtattcgg
    16741 gaaaacttcc gcctcaagcc cagccagtac gctattttca tcaacggcat taaggctgat
    16801 gaagcggata ttccggcgga taaactgatg gccctaccct ccagcgaaac ctacggcgaa
    16861 attgacggcg tgctggggaa cgacccggcg tacgggatgc cggtgacctg gatccagccc
    16921 gcgcagaaag cgaaggcgct gaatatgggg tatcaggtga tcgacagcgc cagcgtgatt
    16981 gccacgcatg tgaataagat tgtgcgtagc tatattcctg atttatttag ctatgacgat
    17041 attacgcagt tgcataaccg tttgtcgtcg atggcaccgc gcctggcgga agatttaagc
    17101 gcggcgctga attacagcca gttgctgaaa gtgtaccgcg cgctgctgac cgaaggcgtt
    17161 tccctgcgcg atatcgtcac cattgccacc gtgctggtcg ccagtagcgc ggtgaccaaa
    17221 gatcatattc tgctggcagc tgatgtgcgc ctggcgctgc ggcgaagcat tacccatccg
    17281 ttcgttcgca agcaggagct gacggtgtat acgctgaata atgagctgga aaatctgctg
    17341 accaacgtgg tgaatcaggc gcaacagggc gggaaagtga tgctcgacag cgtgccggtc
    17401 gatccgaata tgctcaacca gttccagagt acgatgccgc aggtaaaaga gcagatgaaa
    17461 gcggcgggga aagatccggt gctgctggtg ccgcctcagc tgcgcccttt gctggcgcgt
    17521 tatgcaaggt tgtttgcgcc ggggctgcat gtgctgtcgt ataacgaagt gccggacgag
    17581 ctggagttga agataatggg ggcgttgatg taagtatccg gcattggtgc acaactgccg
    17641 gatgcggcgt gaacgcctta tccgtcctac tgttcaggca cgattcgtag gccggataag
    17701 cgcaagcgca tccggcacca gcgcacaacc gccggatgcg gcgtaagcgc cttatccggc
    17761 ctaccgatcc agcacaggtt tgtaggcatg ataagacgcg tcagcgtcgc atcaggcatc
    17821 tgcgcataac cgccggatgc ggcgtaaacg ccttatccgg cctaccgatc cggcacaggt
    17881 ttgtaggcat gataagacgc gccagcgtcg catcaggcat ctgcgcataa ttataaaatg
    17941 atacgctcgc cagcatttat atccgttacg tcccccgccc aatcgatggg atataaccct
    18001 cgcgcgacat cgcgatggaa cgttgagaat ggccaatcac tcacttgctt tacccaacca
    18061 tgctttactg gatttatata aatataatca acatgatgcc tataatcttt ggtattgcgg
    18121 atggcgtgct cccaaaaacg tggttgccag atatttttca atccacaagc atgggtaaat
    18181 tgctttttaa tttcccgcca gcgcgaggaa aaatcatcat cgccttcagg taatgtccag
    18241 atacagtgca tatgctctgg caaaacgacc caggcgttga tttcaaaggg cctgtctcgc
    18301 ttaactttaa taatggcgtg acggagcatc tggtactggg tggtcaagag ttggcttcga
    18361 cgattgcgta aattcaccgt gaaaaaccag gttcccccct tgatgtaata acgacgatat
    18421 tcagacatgg cgactccttt ttctcaacgg taagaagaaa aggaaaggtc ggtaatgagg
    18481 tgatggaata acaggaaagc ggctcgcaaa aacgcacaga tttgtaggca tgataagacg
    18541 cgtcagcgtc gcatcaggca tccgtgcaca accgccggat gcggcgtaaa cgccttatcc
    18601 ggcctacaaa atcgtctctt ccgtcaagat ccgccgcgcg cccaaaaaat gatcctgcca
    18661 gaaaggttcg gctaaccggc ttacccgaat gttttccccg gtgcgcggcg actcgataaa
    18721 ctgcccatcg cccagataca cccccatatg atcggcaatt tcacggctgt ggatatggaa
    18781 aaacagcaaa tctccccggc gcaggtcgtt attcgccaca atcgttgccc gatgatagtg
    18841 atacatctca ttggccgtgc gcgggagctt cgcctcaagg atcttgttgt aggcataaaa
    18901 aaccaacccg ctacagtcga agccttgatc gggccgcgtg ccgccccaga cgtaaggctt
    18961 gccaagctgt tgctccagac ggtgaatcgc cacttcggtg atgttatgca gatggtcgct
    19021 gcttaaaaag tgattgttct ccgccagcgc ctgccagcgt cggtcgctcg ccttgagcgg
    19081 cgcaggaaac cattctggat gctgttttat ctgctcccgg ttgtgctggc gtagcgccct
    19141 tctgctttcg gcattgcctt ccacaatata gctggcctgt tttttcagat gggtttggta
    19201 ctgttttaat aaacgcgcac ggttttgcat ccgctggcgg gcggcgtagc tgaaagagat
    19261 atgtgatgtg gtggcgctgg cgaataaagg gagcaaaacg cataacgaaa acacgcctgg
    19321 atgcagaaat ctgccgagca agaacgaaga catgaaagac ataaaccggc tgaatgaagg
    19381 tgagaatttt aaaagaccgg aggagtaaac gggattatta attgcttatt tgcatattac
    19441 tattgaggtt tcataatctt aggaaataaa tgatacatgc gcttttcgga ctattgaaaa
    19501 ctgcccctgt gctaaatttg taaaccctgc gccgacaggc ttgcgtaaaa ctcttttggt
    19561 gataatgtga gccttccaaa tccagactat tccgatttgg gatccatttt gccgtcctgg
    19621 cgtgtgtaac cgttttatca aggaataagc agtatgcgta aaatcgcatt aattcttgcg
    19681 atgttgttga tcccgtgcgt ttcgtttgcc ggtttgctgg gcagcagtag ctcgaccacg
    19741 ccagtgagca aagagtataa gcagcagttg atgggatccc ctgtctacat ccagatcttc
    19801 aaggaagaac gtacgctcga tctctacgtc aaaatgggcg agcaatatca gctgctcgac
    19861 agttataaaa tctgtaaata ttccggcggc ttagggccaa aacagcgtca gggcgatttc
    19921 aaaagcccgg aagggtttta tagcgtccag cgtaatcagt taaaaccaga cagccgttac
    19981 tacaaagcga ttaatattgg tttccccaat gcctatgacc gtgcgcatgg ttatgaaggg
    20041 aaatacctga tgatccacgg cgattgtgtt tccatcggct gctacgcgat gaccaatcag
    20101 ggcattgacg agattttcca gttcgttact ggcgcactgg tatttggtca gccgagcgtg
    20161 caagtgagca tttacccgtt ccgcatgacc gacgctaata tgaagcgcca caaatattcc
    20221 aactttaagg acttctggga gcaactgaag ccgggttacg actactttga gcaaacccgt
    20281 aagccaccga ccgtttctgt ggtcaatggt cgttacgtgg tcagcaagcc gttaagccac
    20341 gaagttgtgc aaccacagct ggcatcaaac tacacgctcc ccgaggcaaa ataagcgcca
    20401 ttcgcctggc ataatttttt gccaggtttc attgcccgtc agcggctgtg tcgcaatcac
    20461 cgtgaccaca tcattcggtg tggtctgcga gctgaagtcg atttccacat cctgatccag
    20521 caacgttgcc acgccaaacg gcgcgcggcg ggtgatccag tgtaaattag tcgagcaata
    20581 cgccattaca tagcgcccgt cagaaagcag catgttgaaa acgcccttct gccgcagttc
    20641 atccgccagt gaggcgatat atttaaatac cgccgccatg ttgcccggtg tgcgcgggta
    20701 acgctgcgtt aatttatgca ggagccagca aaaggctttt tcgctgtcgg tttcgcctac
    20761 cgggcggaag ttgccggttt ccagtgattt gtagcccgtc agttgtccgt tatgggcata
    20821 agtccagtta cgcccccata actcgcgggt aaatgggtga gtattttcca gcgccacctc
    20881 gccccgatta gcctggcgaa tatgagccac caccgaacag gattttatcg ggtagtcctg
    20941 gacaagtttg gcgatggggg aattaaagct gggttgtgga tctttaaatg tgcgacagcc
    21001 tttaccttcg taaaaggtaa tgccccagcc atctttatgt ggcccggttc caccaccacg
    21061 ctgtacaagc ccggtgaaac taaagcagat atcggtaggg acgttggcgc tcatcccgag
    21121 caattcgcac atgggtaacc tccacaacag cgggagcgca tggcccccgg cgaagattga
    21181 tttaagaatt ttcaggtcgg atgcggcgtg aacgcattat tcggcctacg gttcgcgtac
    21241 agatttgtag gcatgataag acgcgtcagc gtcgcatcag gcatcggtgc acaattgccg
    21301 gatgcggcgt aaacgtctta tccggcctac gccagactta cttaaccatc tctttttcaa
    21361 tcaactggat caggatatgg atcactttaa tgtgaatctc ctgaatgcgg tcggcataac
    21421 caaagtgcgg tacgcgaatt tcgatatccg ccgtgccagc cattttgccg ccgtctttac
    21481 cggtcagggt gatcactttc attcccttct cacgcgccgc tgcgatcgct ttgatcacgt
    21541 ttgcagagtt accggaggtg gagatcccca gcagtacatc gccttcgcga cctaccgctt
    21601 caacgtaacg ggagaaaata tcattgaaac cgaaatcgtt accgacgcat gaaatatgac
    21661 taacgtcaga aatagcaatc gccgggtagc ccggacggtt ttcacggtag cgaccggtca
    21721 actcttcggc aaagtgcata gcgtcgcaat gggaaccgcc gttgccgcag gaaagcactt
    21781 tgccaccggc tttaaagctg tctgctaaca ggaccgccgc gcgctgaatg gcgtgaatat
    21841 tggcgtcatc ttttaaaaag ttagccagcg tttccgccgc ttcgttcagt tcgttacgaa
    21901 taagatcctg gtacatgagg atatccttca gcataaatgt aatagacaaa atgcagtgta
    21961 ccggataccg ccaaaagcga gaagtacggg caggtgctat gaccaggact ttttgacctg
    22021 aagtgcggat aaaaacagca acaatgtgag ctttgttgta attatattgt aaacacattg
    22081 ctaaatgttt ttacatccac tacaaccata tcatcacaag tggtcagacc tcctacaagt
    22141 aaggggcttt tcgttatgat gattttgagt attctcgcta cggttgtcct gctcggcgcg
    22201 ttgttctatc accgcgtgag cttatttatc agcagtctga ttttgctcgc ctggacagcc
    22261 gccctcggcg ttgctggtct gtggtcggcg tgggtactgg tgcctctggc cattatcctc
    22321 gtgccattta actttgcgcc tatgcgtaag tcgatgattt ccgcgccggt atttcgcggt
    22381 ttccgtaagg tgatgccgcc gatgtcgcgc actgagaaag aagcgattga tgcgggcacc
    22441 acctggtggg agggcgactt gttccagggc aagccggact ggaaaaagct gcataactat
    22501 ccgcagccgc gcctgaccgc agaagagcaa gcgtttctcg acggtccggt agaagaagcc
    22561 tgccggatgg cgaatgattt ccagatcacc catgagctgg ctgatttgcc gccggagttg
    22621 tgggcgtacc ttaaagagca tcgtttcttc gcgatgatca tcaaaaaaga gtacggcggg
    22681 ctggagttct cggcttatgc ccagtctcgc gtgctgcaaa aactctccgg cgtgagcggg
    22741 atcctggcga ttaccgtcgg cgtgccgaac tcattaggcc cgggcgagct gctgcaacat
    22801 tacggcactg acgagcagaa aaatcactat ctgccgcgtc tggcgcgtgg tcaggagatc
    22861 ccttgctttg cactgaccag cccggaagcg ggttccgatg caggcgctat cccggatacc
    22921 gggattgtct gcatgggcga atggcagggc cagcaggtgc tggggatgcg tctgacctgg
    22981 aacaaacgct acattacgct ggcaccgatt gcgaccgtgc ttgggctggc gtttaaactc
    23041 tccgacccgg agaaattgct gggtggcgca gaagatttag gtattacctg tgcgctgatc
    23101 ccaaccacca cgccgggcgt ggaaattggt cgtcgccact tcccgctgaa cgtgccgttc
    23161 cagaatggac cgacgcgcgg taaagatgtc ttcgtaccgt tcgattacat tatcggcggg
    23221 ccgaaaatgg ccgggcaagg ctggcggatg ctggtggagt gcctctcggt aggccgcggc
    23281 atcaccctgc cttccaactc aaccggcggc gtgaaatcag tagcgctggc aacgggtgcg
    23341 tatgcgcaca ttcgtcgtca gttcaaaatc tctattggta agatggaagg gattgaagag
    23401 ccgctggcgc gtattgccgg gaacgcttat gtaatggatg ctgcggcatc gctgattacc
    23461 tacggcatta tgctcggcga aaaacctgcc gtgctgtcgg ctatcgttaa gtatcactgt
    23521 actcaccgcg ggcagcagtc gattattgat gcgatggata tcactggtgg caaaggcatt
    23581 atgctcgggc aaagcaactt cctggcgcgt gcttacctgg gcgcaccgat tgccatcacc
    23641 gttgaagggg ctaacattct gacccgcagc atgatgatct tcggacaagg agcgattcgt
    23701 tgccatccgt acgtgctgga agagatggaa gcggcgaaga acaatgacgt caacgcgttc
    23761 gataaactgt tgttcaaaca tatcggtcac gtcggtagca acaaagttcg cagcttctgg
    23821 ctgggcctga cgcgcggttt aaccagcagc acgccaaccg gcgatgccac taaacgctac
    23881 tatcagcacc tgaaccgcct gagcgccaac ctcgccctgc tttctgatgt ctcgatggca
    23941 gtgctgggcg gcagcctgaa acgtcgtgag cgtatctcgg cacgtctggg tgatatttta
    24001 agccagcttt acctcgcctc tgccgttctg aagcgttatg acgacgaagg ccgtaatgaa
    24061 gccgacctgc cgctggtgca ctggggcgta caggatgcgc tgtatcaggc agaacaggcg
    24121 atggacgatt tactgcaaaa cttcccgaac cgcgtggttg ccgggctgct gaatgtggtg
    24181 atcttcccga ccggacgtca ttatctggca ccgtctgaca agctggatca taaggtggcg
    24241 aagattttac aagtgccgaa cgccactcgc tcacgtattg gtcggggtca gtacctgacg
    24301 ccgagcgagc ataatccggt tggtttactg gaagaggcgt tggtggatgt gattgccgcc
    24361 gacccgattc atcagcggat ctgtaaagag ctgggtaaaa atctgccatt tacccgtctg
    24421 gatgaactgg cgcacaatgc gctggcgaag gggctgattg ataaagatga agccgctatt
    24481 ctggtgaaag cggaagaaag ccgtctgtgc agtattaacg ttgatgactt tgatccggaa
    24541 gagctggcga cgaagccggt aaagttgccg gagaaagtgc ggaaagttga agccgcgtaa
    24601 ttgaataacg gagccgaaag gctccgtttc tttatccgct aattatttaa aattaaagcc
    24661 atccggatgg ttttccaggc tgccggtcaa cgccgcgaac agcaccgttt taccatcaat
    24721 cgaaagcgca tcgttcacat tcagccaggt gagtttctct tgcgacgttt tctcatcaat
    24781 agtcgagaac agccccgtca tctgattaga tttctcggac cacatcacag cgatacgttg
    24841 cgagccacag tcatgcggtt tgcacgcgct catcacctga tacgtctcat ctcccaacgt
    24901 tacggtttgt gcgggagtat aagtaccgcc tttcatcacc catgcaggta gcttatgccc
    24961 ttgcaccatc tgattaaacg cagctttggt ggtttcgccc tttgccaggc tgctaatggt
    25021 taaatcatcc tgcgccattg cactggtggc gatgaccaga gcggcgactg tcgttattgc
    25081 cttaaacatc attcctcccg agcttatcct gcccatgtta ttaacctcct aaagcctggc
    25141 gataacacga taatttgcaa atcagatatt tccaatatca gcatttgtta tcaatttatt
    25201 ctgcgctaag gcaactgcct gataaggtgt taaagttata aaacccttcg cataaaggag
    25261 ttgtacaccg tgcctggttt gaagattacg cttttgcagc aaccactggt gtggatggat
    25321 ggccctgcca acctgcgtca ttttgatcgt caactggaag gtattaccgg gcgcgatgtg
    25381 atcgttctac cggagatgtt taccagcggc tttgccatgg aagcggcagc ttcgtcgcta
    25441 gcacaaaatg acgtagtgaa ctggatgaca gctaaagcgc agcagtgcaa tgcgctgatt
    25501 gcaggcagtg ttgcattaca aacggagtct ggttcggtta accgcttttt gctggttgag
    25561 ccgggcggca cggtacattt ttatgataag cgtcatctgt tccgcatggc agatgagcat
    25621 ctgcattata aagcgggcaa tgcgcgagtg attgtggaat ggcgcggctg gcgtattttg
    25681 ccgctggtgt gctacgactt acgttttcct gtgtggtcgc gcaatctcaa cgattatgac
    25741 ctcgccatct acgttgccaa ctggcctgct ccgcgctctc tgcactggca ggcattgctg
    25801 acggcccgcg cgattgagaa tcaggcgtat gtggcgggat gcaatcgcgt cggcagcgat
    25861 ggcaacggct gccattatcg cggtgacagc cgggtgatta acccgcaggg cgagattatc
    25921 gctactgccg acgcgcatca ggcaacgcgc attgatgcgg agctgtcgat ggtggcattg
    25981 cgggaatata gagaaaagtt tccggcatgg caggatgcgg atgagtttag gttgcggtga
    26041 gtgggggttg tgtttaaggc tgggggagag tcggggtaag actacgaaag cccgctctcc
    26101 gcaaggactg acgccagata gtttctgtcc atagctgctt ttcgcatctt acgtcttaac
    26161 cctgccttga ataccttatc attcgtcaaa atattaatag cgatgtgccg tatccctgaa
    26221 aataattctg cggcgtttcc tcttcttatt ttgcagtcgt cttcattcat taccacgtcc
    26281 agacgccagt gcagcttatt ctccacgtgc cagtggtttc ggattgctgt ggcgaacttt
    26341 tctgcggtta aatcagcaga actgatataa tatctgaccg tcatttctgg ctctttcttt
    26401 tgttctgcta ttattgaccg aaaggagact gccacgcata atttcttcag tcctttccat
    26461 tcaaacgtga aatcaataag ttcatcaggg acatcgcaaa caatatgaag acggatttct
    26521 tctctgccgt gac
//
LOCUS       DAMZET010000049        26366 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.049, whole genome shotgun sequence.
ACCESSION   DAMZET010000049 DAMZET010000000
VERSION     DAMZET010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.049"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..253)
                     /locus_tag="POB34_002128"
     CDS             complement(<1..253)
                     /locus_tag="POB34_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein B"
                     /protein_id="HDI5919071.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHV"
     gene            complement(258..1511)
                     /gene="pqiA"
                     /locus_tag="POB34_002129"
     CDS             complement(258..1511)
                     /gene="pqiA"
                     /locus_tag="POB34_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HDI5919072.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(1641..3548)
                     /locus_tag="POB34_002130"
     CDS             complement(1641..3548)
                     /locus_tag="POB34_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5919073.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYLGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(3560..5668)
                     /gene="rlmKL"
                     /locus_tag="POB34_002131"
     CDS             complement(3560..5668)
                     /gene="rlmKL"
                     /locus_tag="POB34_002131"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HDI5919074.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLLVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLVGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            5912..7021
                     /gene="ycbX"
                     /locus_tag="POB34_002132"
     CDS             5912..7021
                     /gene="ycbX"
                     /locus_tag="POB34_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HDI5919075.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            complement(7018..7560)
                     /gene="zapC"
                     /locus_tag="POB34_002133"
     CDS             complement(7018..7560)
                     /gene="zapC"
                     /locus_tag="POB34_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HDI5919076.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAMKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7734..8744)
                     /gene="pyrD"
                     /locus_tag="POB34_002134"
     CDS             complement(7734..8744)
                     /gene="pyrD"
                     /locus_tag="POB34_002134"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDI5919077.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(8855..9592)
                     /locus_tag="POB34_002135"
     CDS             complement(8855..9592)
                     /locus_tag="POB34_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDI5919078.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADKESIAILLTNTSQESWLINSKINRPTRWAGGEVSTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     VIAQCHVAWRAINDYGGLSAKKEQNLP"
     gene            complement(9558..10073)
                     /locus_tag="POB34_002136"
     CDS             complement(9558..10073)
                     /locus_tag="POB34_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI5919079.1"
                     /translation="MLKRIIWILCLLGLTGSSELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGIPDGVQSEMLSI
                     ESGMDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(10081..10623)
                     /locus_tag="POB34_002137"
     CDS             complement(10081..10623)
                     /locus_tag="POB34_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI5919080.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(10635..11705)
                     /locus_tag="POB34_002138"
     CDS             complement(10635..11705)
                     /locus_tag="POB34_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI5919081.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            complement(11696..14296)
                     /locus_tag="POB34_002139"
     CDS             complement(11696..14296)
                     /locus_tag="POB34_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HDI5919082.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNHLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            complement(14321..15022)
                     /locus_tag="POB34_002140"
     CDS             complement(14321..15022)
                     /locus_tag="POB34_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDI5919083.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            complement(15105..15647)
                     /locus_tag="POB34_002141"
     CDS             complement(15105..15647)
                     /locus_tag="POB34_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI5919084.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            16003..16578
                     /gene="ssuE"
                     /locus_tag="POB34_002142"
     CDS             16003..16578
                     /gene="ssuE"
                     /locus_tag="POB34_002142"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HDI5919085.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            16571..17530
                     /gene="ssuA"
                     /locus_tag="POB34_002143"
     CDS             16571..17530
                     /gene="ssuA"
                     /locus_tag="POB34_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HDI5919086.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKT
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            17527..18672
                     /gene="ssuD"
                     /locus_tag="POB34_002144"
     CDS             17527..18672
                     /gene="ssuD"
                     /locus_tag="POB34_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HDI5919087.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            18683..19474
                     /gene="ssuC"
                     /locus_tag="POB34_002145"
     CDS             18683..19474
                     /gene="ssuC"
                     /locus_tag="POB34_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HDI5919088.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            19471..20238
                     /gene="ssuB"
                     /locus_tag="POB34_002146"
     CDS             19471..20238
                     /gene="ssuB"
                     /locus_tag="POB34_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HDI5919089.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(20445..23057)
                     /gene="pepN"
                     /locus_tag="POB34_002147"
     CDS             complement(20445..23057)
                     /gene="pepN"
                     /locus_tag="POB34_002147"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HDI5919090.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            23323..24525
                     /gene="pncB"
                     /locus_tag="POB34_002148"
     CDS             23323..24525
                     /gene="pncB"
                     /locus_tag="POB34_002148"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDI5919091.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            24694..26094
                     /gene="asnS"
                     /locus_tag="POB34_002149"
     CDS             24694..26094
                     /gene="asnS"
                     /locus_tag="POB34_002149"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDI5919092.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         6378 a         6879 c         6450 g         6659 t
ORIGIN      
        1 caacgtgcgt caaatcatca gccagtgtgg cgctttcaac cacgccgacg tcaacgctac
       61 ggcttttaat ggtggttttg ccaccttcaa ttccttccgc attcgcggtg atcagggtca
      121 cttccggtcc ctgatggctg taatgataaa aaagaaccca ggccccaatg agcgccgtga
      181 cgataggaaa tatccacacg ggagaccagt tcttcacttt ctggattttg gcttccccat
      241 tattagattc catactgtca ggactcctca tgctctgatt caggttgacg atcccacgac
      301 aaacgcgggt caaacgtcat agcagaaaac attgtcatta tgacgactaa agcaaacatt
      361 aatgcaccca ttgccggata aatactcatt aaacctccca tacgcaccag cgccgagagc
      421 accgcgataa cgaaaacgtc aatcatcgac cagcggccta caaactcaac aacttcataa
      481 atcaaatgca ttctttcact gtcgcgcttg ccatgccctt tggcatccca acacagccac
      541 gcgatggcga tcatctttaa cgttggcacc ataatactgg ccagaaagat caccgcagcg
      601 acgggataag agccttcgct ccataacaga atgaccccag cgagaatcgt cgacggcatc
      661 ttcgagccta ataaatccgt caccatgatg ggcaaaatat tagccggaag atacagcatg
      721 atggacgtta caagcagcgc gagtgtccac tgcaggctgt ttctacgccg aacgtaccct
      781 ttggtactac aacgcgggca cacgggttca tcagcaggaa ggattgccgt acaacaggag
      841 cacgaacgca gcccctgacg tatccccgtg acgcctggtt ttagcggctg gcgcagttct
      901 ggcatcgggg cgatgtcgtc ccataaccag cgacgatcaa cgcactgaaa agcgcgcagt
      961 tgcaggacac aaaataaaca ccaggggaga aagctgctgc ctaccccaat gctgccgtaa
     1021 gccatcagtt taacgaaact gaccagcaca ccagcgagga aaatctccgc cattccccag
     1081 gttttgagtt gaaaaagcac ccgtgccagt tgctctttta aacggaccgg taattccgcg
     1141 cgattcacca gtaacagaat ggttatcaga caaaacgcgg gaaccagttg cacaaacaac
     1201 aggaaaaagg tgccgaggct ggcgtagtcc tcagaaaaaa gcacgccggg aatttccagt
     1261 aatgtaattt cactggtaac tcctgcaacg ttcatattca caaaaggaaa caagttggac
     1321 agcaacagca tgaacagtgc agccaacgca taggcggtgg gacgctgccg aggggcatcc
     1381 cacgccacgg ttaacgttgt gccacaccgg ggacatgccg ctttctgacc atgctcaagg
     1441 cgcggtaacg ccaccagcat gtcacactgc gagcacagga tgtgcttcgc ggcatgatga
     1501 tgttcgcaca ttggtactcc ttaatgatgc gctattttac agatgactaa caaagtgttg
     1561 gttttcatgc ctgataaacc cagttcataa ggcaatttta ccgttgtcag ctgtctctgt
     1621 ttaaatcgac tattttgcgc tcagccacca ttttttaacg cttcaagata ctcccagcgt
     1681 tcaaaggctt gctccagctc ctgctctgca gcagccatat cggcgagcac tttttgtgtc
     1741 tgctcatgtg gctgactgaa gaaggaagca tccgccacct gcgtctgtag ggcttccagc
     1801 ttcgcctcca gatcttcgag caattgcggt agctgctcca gttcgcgctg caatttatag
     1861 cttagtttgc tactgctgcg ttttacagtt tctgcttttg ccgcggcggc ttcttcggtt
     1921 tttttcaccg caggctgttt gagcgccaca tactgctctt gctgaccacg ggcatcatga
     1981 taaccgccga cataacgacc aattttaccg ccgccttcga agatccaaca ttctgtaacg
     2041 gtgttatcga caaactgacg atcgtggcta accagcaata ccgtgccctg atagctgtcg
     2101 atcagttctt ccagcagttc cagcgtttcg acatcaagat cgttggtcgg ttcgtcaaga
     2161 atcaataagt tgcttggttt gaggaacaaa cgcgccagca gcaagcggtt ccgctcaccg
     2221 ccagaaagcg cacgtaccgg cgtcatcgcc cgtttcggat ggaacagaaa gtcctgcaaa
     2281 tagcccaata cgtggcgtgg cttgccgtta accatcacct cttgcttacc ttcggcaagg
     2341 ttatccatca ccgttttatc gggatccagt tccgcgcggt gctgatcgaa ataagccact
     2401 tccagtttgg tgccaacgtg aatacgcccg ctgtccgctt gaagctgacc gagcatcagt
     2461 ttcagcagcg tggttttgcc gcacccattc ggaccaatca gggcaatttt gtcgccacgt
     2521 agaacctggg ccgaaaaatc tttcaccagt tgcttaccgt caacctggta gcaaacgtct
     2581 tccatttcga aaacgatctt accggagcgg ctggcctctt ccacctgcat ctttgcggta
     2641 cccatcactt cgcgacgttc accacgctcg cgacgcatcg ctttcagggc gcgtacgcgg
     2701 ccttcattac gggtacggcg tgctttgatc ccctggcgga tccacacctc ttcctgcgcc
     2761 agtttgcgat cgaactcggc attttgtaat tcttccacgc gcagggcttc ttctttttcc
     2821 agcaggtact ggtcgtaatt ccctagatag gtcaccagct tgccgcgatc gagatcaaca
     2881 atgcgcgtcg ccatattgcg gataaacgaa cggtcgtggg agatgaaaat aatcgtcccg
     2941 ttgaaagttt tcaaaaaccc ttccagccag tcgatggttt caatatccag gtggtttgtc
     3001 ggttcatcaa gcaacagcac gcgcggatta ctcaccagcg cgcgtcctaa tgccgcttta
     3061 cgcaaccagc cgccggaaag cgacgacagc gcaacgttag gatctaaccc cagttgcgcc
     3121 agcacttcgt tgatgcggtt ttccagctgc cacaggttgt ggtgatccag ctgttcctga
     3181 accttcgcca attcgttgag atttttctcg ctcgggtcgt tcatcaccag gcgcgaaata
     3241 tcgtgatagc gtttcagata ttccgcttgt tcttcaatgc cttcggcaac gaaatcataa
     3301 acgctaccct caacgttacg cggcggatcc tgttgcagac gagctacaat caaatcttgc
     3361 tcgtaaataa tgcgaccgtc atccagccct tgttcacggt tgaggatttt cattaacgtc
     3421 gatttgcctg cgccgttgcg gcctaccaga caaacacgtt cattatcttc gatatgcagt
     3481 tctgcgttat cgagaagcgg cgcgtcgctg aacgacagcc aggcgccatg catactgatt
     3541 aatgacatta ctattccttt caggctgcgg taatcagcca gcagttgtgg atctggcggt
     3601 tacgggcgaa atcctgggag agcgtttttt gcgtaatttc ttgtgctttc agtcccagtt
     3661 ttgccaggcc gacgagatcc atacggaagc cacgtttgtt gttcgagaac atgatcgtcc
     3721 cacctgcacg cagcagacgt ttcaaatctt tcatcagcgc cagatgatcg cgctgaacat
     3781 caaacgcatc ttccattcgt tttgagttag agaaggttgg cggatcgata aagatcagat
     3841 cgaactgttc atttgcctca cgcagccacg ccaggcaatc ggcctgaatc aggcgatgcg
     3901 cacgcccggt caggccattc agacgcaggt tgcgttctgc ccactccaga taagtacgcg
     3961 acatatccac ggtggtggtg ctgcgtgcac cgcctaatcc cgcatgcacg gtggcgctgc
     4021 cggtataaga gaacaggttg aggaaatctt tgcctttgct catctggccg agcatacgac
     4081 gggcgatacg gtgatcgaga aacagaccgg tatcgaggta atccgtcagg ttcaccaaca
     4141 agtgagcgtt atattcggta acttcaagaa actcgccctt ctcgcccagt ttctggtact
     4201 gatttttgcc cttctggcgt tcacgggttt tcagcaccag tttgtttggc gcaatcccca
     4261 gtaccgaaat ggttgcagcg ataatatcga acagacgctg acgcgctttg tgagcatcaa
     4321 tagtttttgg cggcgcatac tcctgcacca ccacccagtc ggcataacgg tcaacggcaa
     4381 cgttatattc cggtagatcg gcgtcataca ggcggtaaca ttcaattcct tcctggcgag
     4441 cccacttctc gaattttttg aggttcttac gcaggcggtt ggcgtagtct tccgctacca
     4501 tcgccggttt gctgtctgga gtgctttcgg caacgtggta atttttctgt acgcagtcca
     4561 gcgggccgtt tttcgccttg tactgtttgt ctgcacgcag ctgcaagcag cttagcagat
     4621 ccggcgaggc actaaacaaa gagagattcc agccaccaaa ctggtttttc atgatccgac
     4681 ccagcaggct atgcagcgca atcagcgccg gttcgctgtc cagacgttcc ccgtatggcg
     4741 ggttgctcaa cactgtgccg tacggccctt tcggcagcgg attggtcagt tgcgcgacat
     4801 ctttcacctc aaaggtgatc agttcaccaa tccccgcaag acgggcgtta gtgcgtgcac
     4861 gttgaatcac ccgtgcgtcg ctgtccgaac cgtaaaaatg agagctatac tcagccaggc
     4921 ctttacgggc gcgagtttgc gcttccgctt tcacttcctg ccagatagct tcatcatgct
     4981 gcgcccagcc gctaaagccc caacgcccac ggtgcaagcc tggtgcgcga tcggtcgcca
     5041 gcatcgccgc ttcaatcagc aacgtaccgg aaccacacat cggatcgagc agcggtgttc
     5101 ctggctgcca gccggatcgc atcacaatag cggctgccag ggtttctttg atcggcgcaa
     5161 taccagcacg atcgcgatag ccacgcagat gtaaaccatc accactcaga tcaagagcga
     5221 tactggcggt ttctttatgc agccagacgt taacgcggat atccggcgca tcgcgatcaa
     5281 catttggacg cggcagattt ttccgcgtga aagcatcgac gatcgcgtct ttcactttca
     5341 tcgcgccgta ctgactgttg cggatggtgt cattcaaacc actgaagtgg acagcgaagg
     5401 tcgcgccagg attaaacatc tctgtccagt tgatcgcctg aacaccgaga tagaggtcta
     5461 aatcgctgta aaccttacac tcgcccagcg gcaacataat acgcgaggcc aggcggctcc
     5521 acatcaggct ctggtaaaca agccgtgtgt cgcccttgaa atggacccca ccctgaacca
     5581 cctggcattc aacggccccc aggttttcca gttcagtttt taacagctct tccagcccac
     5641 gggccgtact ggcaaacaga gaattcatat cgtcactttt actctaagag cctaccgcag
     5701 taggcgtaat tgttgcagtc agtctggaca tggacagcgc ggagaaaccg gagcgtacac
     5761 aagtacgtga ggatttcgag cacaacccag ggccaaactg acaaataaaa tagcctaatg
     5821 ggacaggcgc taagaaaatt gttgcgcatt atagctaata agcgcgtcat gtcataaagt
     5881 tgagggctta ttttcatttg aggaccgcac cgtggcgaca ttaacccggc tttttattca
     5941 tcctgttaaa tcgatgcgcg gcattggtct tacacatgct ctggctgatg tcagtggtct
     6001 ggccttcgat cgcatcttta tgatcacgga acctgacggt acgtttatta ccgctcgcca
     6061 gtttccccag atggtacggt ttactccttc acctgtgcat gatggcttgc atctcaccgc
     6121 accagatggc agtagcgcat atgttcgttt tgctgatttc gccacacaag acgcaccaac
     6181 cgaagtttgg ggcacacatt ttaccgcgcg aattgcgcca gacgcgatca acaaatggct
     6241 aagtggattt ttctcccgcg aagtgcaatt acgctgggtg gggccacaaa tgacccggcg
     6301 cgtgaaacgc cacaacactg tacctctgtc atttgctgat ggctatcctt accttcttgc
     6361 taacgaagcc tcgttacgtg atctccaaca acgttgtccg gccagtgtaa aaatggagca
     6421 attccgcccc aatctggtgg tttccggcgc gtcagcctgg gaagaagatc gctggaaagt
     6481 gattcgcatt ggtgatgtgg tgtttgatgt ggttaaacct tgtagccgct gtattttcac
     6541 caccgtcagc ccagaaaaag ggcaaaaaca tccggcaggc gaaccattaa aaacattgca
     6601 atctttccgc actgcccagg ataacggcga tgtcgatttt ggtcagaatt taattgcccg
     6661 taatagcggc gtgattcgcg ttggcgatga ggtggaaatt ctggcaacgg ctccggcaaa
     6721 aatttacggc gcagctgccg ctgatgatac cgccaacatc acgcaacaac cggacgcaaa
     6781 tgtggatatt gactggcagg gacaggcatt tcgtggaaat aaccaacagg tgttgctgga
     6841 gcaattagaa aatcagggaa ttcgtatccc ttattcttgc cgcgcgggca tttgtggaag
     6901 ttgccgtgtt cagcttttag aaggcgaagt cacgccgctg aaaaaatcag caatgggcga
     6961 tgatggcacc attctttgct gtagctgtgt accgaagact gcacttaagt tggcgcgtta
     7021 gactgcctgt tcgaggctga agctgtcaac attaacctgc ggtttcagcc tgtcgttcat
     7081 gattttcatg gcgtcgccca actgcatcgc gcgacccgca ataacaacgc agggttgtgc
     7141 cagcaggcat agtgcggcat tttcgccaga ttcaacgacc agcaaattga cctgctcgtg
     7201 agtatcgctc aaccaaacgc aggccgcatc gcccggcatg ggcacccaca tttcaccatg
     7261 ggaaacaaaa tgccagcttt tcggcatttg cggtttgaga tagcgaattg ctaccagtgc
     7321 attcagcacc agttcggctt tttgttcttt agataaatta aaatcgcggc acttttcttc
     7381 aaaagagaaa tagagcgcgg cgtcgtcaac gcaaaagcct gcgggagaaa aggcatctgg
     7441 agtcaacatc ttgcgcgcaa aacgtgagcg aaatagcata ccattggcta aatcgagcat
     7501 cattcgatcg tgctcttcat cgtaatacca acgccagtta tcgtctggtt taattcgcat
     7561 ctgcctctcc cctaccctat aaaaccgcag caacacaatc cattgtcgct tcgcttaata
     7621 acctcaacaa taaaagtaca aaatgtctaa ataagcaaca gcaatgaata taaaacaacc
     7681 agggctgaaa ataaagccct gattatctta taaagaggaa ttcgaagaaa taattagata
     7741 tgggtaacga tttctttaat cagcggcgga cctttaaaaa taaaaccaga ataaatttgc
     7801 accagtgagg cacccgcagc aatcttttca cgcgcagcga taaccgagtc gatgccgcca
     7861 acaccgatga tcggtaagcg accgtttaat tccagtgaca agcggcgaat aatttcggtg
     7921 ctttttaact gaagcggacg accacttaag ccaccggttt gatcgcaatt tttcattccc
     7981 tgaacaagag aacgatcgag tgtggtattg gttgcaataa cgccatcaat attatggcga
     8041 actaaactat cggcaacctg gatcaattct tcttcagaaa gatccggcgc gatcttcact
     8101 gcgatcggca catatttatg gtgcatcgct tgcaaatcat tttgcttatt tttaatcgcg
     8161 gttaagagat catccagcgc ttcaccatat tgcagcgtgc gtaatcctgg ggtattcggc
     8221 gatgaaatat tgatggcgat atatcccgca taggcataga ttttttccat acaaatcaga
     8281 tagtcatctt tgccctgctc cactggcgta tctttatttt tgccgatgtt aatacccagg
     8341 acgccgtcat aatgggcctt ttttacgttc tctacgaggt tatcaacgcc aagattatta
     8401 aagcccatac ggttgatcaa accttcggca tctaccagac gaaagagacg cggcttgtca
     8461 ttacctggct gtggacgtgg cgtgacggta ccgatctcga tcgatccaaa tcccatcgcg
     8521 cctaacgcgt caatgcactc cccgtcttta tcaagaccgg ctgccagacc aagcggattt
     8581 ttaaacgtca ggcccatgca gttaacaggt ttcgcaggca ctttctgccg caccagtgct
     8641 tcaaacggcg ttcctgtaat acggcgtaat tgctgaaaag taaactcatg agcgcgctct
     8701 ggatcgagct ggaaaagggc tttacgaacg aaggggtagt acatgaactc tcctggattc
     8761 ccggtgtgca aaccgggggc gtattatgtg cgagttcgga ccaaaaggga attgacctgc
     8821 ggcaaaaaaa acgcaaacgt tttccgaacc tgtttcaggg cagattttgc tccttctttg
     8881 cactcaaccc gccgtaatca tttatcgccc gccaggcaac atgacactgt gctattacct
     8941 gacaccagct tgtctgacgc tggcttttag gtggcacaac tcccgcatta cttaacgctt
     9001 taccatttac gctcacctga atcaggttaa tgtagtaagg cgttgggtta gtgagttgca
     9061 caccctgcga attccgtgtc cagcgtaatt gttgataagc ttccagcggg tcgcccggca
     9121 aaccttcggg tcgccagaac agtttaaata ccgagcgcat cgccactttt acgctctgat
     9181 tttcaacttt gccggatggc acgctggcaa tgcttaactc aaatagcgtt tcgcgatcca
     9241 ctggcaagat gtcgctttcc gttctcagca agcgcaacgt gcccgtagta ccgggcttca
     9301 ggagaataag cggtggagcg gccagtaatg gtgctggcac tgtcgacact tcccccccgg
     9361 cccaacgcgt tgggcgattg attttactgt taataagcca ggattcctga ctggtattag
     9421 tcagtaaaat agctatcgat tctttgtctg ccggaaagat aaatcgcgtt ccgccgacca
     9481 ccactcctgc ctgcaattca cccgccacca gcagcgataa aacggcaaaa atcaagagcg
     9541 ccaatctgtt ccacgtatta gtcataatga agtacaaatg tcgcgctggc attcacttta
     9601 cccgactgca catcactatt gacaggtcgt aattgcgcat aaaaagttaa tggcacctta
     9661 ccgctgtgta ggctgtagtc tttactcgcc tggttaagcg gaatcaatat cttcgcatca
     9721 tccagaatcg caattgccag cccgctggct gcatccatac cactttcaat ggacagcatt
     9781 tcgctctgca ctccgtcggg gattcctgaa aaggtgacgt caaccgtgct gacatctgtt
     9841 ccgcaatcct gcaaattaat gatgaaagat ttctccgggc cataattccc atgaaaaaaa
     9901 cgtgtcagtg agacatcgcc taactgcaca ttgatttgct tgctatcctg tgccagcacg
     9961 catgtattac gacgaaacga tccgctaatg ttgaccgtgc catcatgagc aaataactca
    10021 ctactccccg ttaatcccag taagcacaga atccagatta tccgtttcaa catcactcct
    10081 tcactggtaa accagatcaa aatagagaac cgccgtcgca ttaccgcccg ttgctcccgc
    10141 ctttgtggac ttataacgaa gttgatattt gagtgtgtta tccccggctt ttaagggcgt
    10201 aagaggctgg acctgattga gcgggatttc ttgctgactt tgcgcatcaa gaatttgcac
    10261 ggcaatcccc gttgccatac caccgcttcc gcctgtgtca gttagtttaa gcagtgtcgg
    10321 cgcaagggtg tcagcctcgc cgctaaaaag gacgtccgcg ccagtaatac cagcggcaca
    10381 accggtgata tcgatattca aatcagcagc agtgcttacg gtattcgcgg cctgaaaatc
    10441 actgatatta aaatcaccaa tatgaatatt ttgtaatgca cttttggtgg cgacatcaca
    10501 gccctgatcg accacattcc cataaatatg catactaact tgttcaggcc cagccatccc
    10561 gacatggatg ttaagaatgc taaagaacaa gataacgcac tgaaatatcg tttttttctt
    10621 catacgttca cgcgttaatc aaaatttatt ctcaccgttg ccgtggcttc aaatcgcccg
    10681 gtttctggtg gtacacctgt cgtgctgact ggatagacat tcattcgcgc gatactgtct
    10741 gcatcatcag gaatagagag cggtaatgtt ccaccagcag gggaaataat gttgttctgg
    10801 ctatcggtca ccaccacgcc cacgccagga cgtgacgtct taatcgcctg gggatagctg
    10861 gggtctgtgg ttgccgtcag actcaggttg acgttaacta accccgcgtt tcccgtgtta
    10921 cacctgactg gaattgcaag ttctgccgtt ctggccccaa gaggcttatg ccctacaaca
    10981 cgaaagttag ccgcaaatac gtcgcccaga tcgacctcga ttgtttctcc ggcattgacg
    11041 gaacatgagc caagtgaatt gatcgtacca gacaggtaat acactaaaac cggtgtacct
    11101 gtagtcgtgc aggaatcacc tgcgggtatg ttgtagcatt caaataatcg cgccagcgcc
    11161 ataggaggaa tgacgaccga accaacgaac ggctgcaata ttttcaggga aaagctaccg
    11221 ctattaccgc tagccccctg gccacctaaa cgttgaccgc cgggatcgca aatatgccat
    11281 cgtgtatctg cccctgtgcc cacgttttcg aaaggaaccg cagtaggaga actgccttta
    11341 acctgcaata aaactttggc ggcaagatag tcattaattt tgtaccagtt gtcgcctaaa
    11401 tagactaacg gtgaatccgc ggcgaatgcc catcggccac ttctgacatt gtctttatcg
    11461 caatcacagg aaacataata atcattcgat tcgttccact gataaaattt gtctttaact
    11521 actccagccg tattttcttc ttcctccagg gttttaacgc caaaattgac gccaatatac
    11581 ggattcccct gagtggtatg acactgcccg ctataagcag cagtttgagc gcaccatagc
    11641 attaagacgg cggcaaaaaa tagtcgtagt aataacacgc atttttctcc aaagattatc
    11701 tgcatatggc atccattcgc gtaatcgcag agttaatttg ttgttcggta agctggtaac
    11761 tgatatgaca aatttgatcg ggagcttcgc cccagcgtac agttatcgct ccctgagcgg
    11821 gcaaaccgga catatagagc gagccatcat catcaataat gccgctatta gtctgtatgc
    11881 cgtccagcgt cgctatcgcg ccaaaacgca gcggtatacc ctgctttgat gttttcatta
    11941 atacccttgc tcccgcatgg gcattgaact ccgccagaac caacgcccct ttggtgggaa
    12001 ccacgttgac tactgcattt tcaagatcca cgttacgttt taacgatgcc gcatcaagtg
    12061 cgattcgatt ttcccgataa gaagtggcat aggggacaac ggtatatccg cgccagtcag
    12121 ttttcagccc ggtgctgttc gccacacgag tattctctgc gcctggtgct gcaatcagca
    12181 cgttagtttc ccccagcgat tgccccaggg taatgccctg tgaatgggca actaaactgc
    12241 ctgaaagagc gtagttcagt tgttgttgac tgccgttatc gctgtagttg tagcccactc
    12301 gggcatctgc aaacgcccct ttgtagtcca tgctggcgct accattagcc gttttcccct
    12361 cgctgttata tccctgttgc acgctgtagc tcaggttacc gtcatcgagt agtgtctccg
    12421 ttaaacccag gttttgtgta atgtgccctt cgttatcgat actggtgtta gaggtcatcc
    12481 aggcgtaatt ttttttcctg gtatagctat cattacttct ggggagcaac agattgagcg
    12541 gcagggaaaa atttagtgca aacacctgat cagcatcagg ttgaccacgg gacttactgt
    12601 agttccagga aacccccagc gagagatctt taatttgcgt gttgtagcca aactgtaata
    12661 gccgatcctg ttgatcggta tgccagtaag tttgctggct accacttaaa taaaacgaac
    12721 cgtactcgcc taattgctgg gagatattga cctgcagttt gccacgtttg gtgtaaaaca
    12781 aattgtaata acgcgaccac atcggcgtat cttcatcatc accgtcatta aattcgtagc
    12841 catccatatg tttatacatg gtgtcggaaa gggtgtagaa acccgacgtc gaatagcgat
    12901 agcccatcaa ttgaaagtta gtgcccaagt ggttgagcgt tttggcatac agaaaacgat
    12961 atgactgacc tttatgttcg ctttgatctg ccagcgtact cttcgcctgg gtcgcatcga
    13021 acgaaattgc tccgaaatcg ccgaggttaa atcccagacc aaacatggcg gcacggtaat
    13081 attcagcata ttgtccacca ccataccagg tcgttcccca cggtccgccc cactgcaacg
    13141 tggcctgggc aaatttgctc tcctgctgtt cattactatt ggttctgtat ttcgccagtg
    13201 tcaccgcgta tttgattcgc ccctgacgct ggagtaatgg cacgctggaa aacggtacgc
    13261 tgtagctatt gacgctgccg tccgcttctt tgatttcaac taacaaatca ccgctcgacg
    13321 acgtggaata gagatcacta atttcaaaag caccaggcga tacataagtt tggtagattg
    13381 tgtagccgtt ttgcttaatc gttatctggg cattactttt ggcgatacca cgcaccacag
    13441 gcgcaaaccc tttcaggctg tctggcagca tgttgtcatc tgacgccagc tgcacaccac
    13501 gaaagctgac actatcgaaa aaatcgcctg cagtataatc atcacctacg gtcagttcgc
    13561 cttttagtgg aataaccgcc cgctgcaaat agctgctgag attcttccat tctgcggttt
    13621 ggccactact gcgactccat gttgaattgt tgcgcaatct ccatgggcct aaattaacgc
    13681 cactgtttaa attcagaaaa tagctgtcgc cagaatcact gtctgcgctg ctatgaatac
    13741 tgttagcccc gctaaacgaa tatcccagta atagcgcgtt gatcccttca tcccaacgtt
    13801 caggggggac ataaccacgg gcattacttt tgatggcgat ttgcggtact gaaatttcca
    13861 gacggagttt attcacatta aacgttacgc tggcatccgg aataatctcc gccaatggca
    13921 cacacgcttg tttgtccagt tgtttaaaag ccggaaacgc atcagtatta acacccattg
    13981 catccaggct ttcagtcgtc aaacaggcgg caatcccctt ctctggcgtt atctcaacaa
    14041 aattgacgtt gcgcgtatct acaattgtct gattcagaac aatatcgaca cgataaaccc
    14101 ctggcgttat tttttgtcct ttttcgaaac gagacaaatc ggccaccatt gagggatcat
    14161 ctttgaaaaa agcaggatcg aaccaggttt cagcttgtgc attgaacgct gccgacacaa
    14221 acaccaccag cccaccaata aacgatggca ctccaccaga gcttaacagg ctgtgttgtc
    14281 gatgagttct atacatctcg ctctcttaac tgaagacggc ttacgattta cagttgagtt
    14341 gcggcgtttc cgccccgtag tcattgatgg ttcgatacgt tacgctgcta tttttggccc
    14401 cctttggcag agagaattgg tatttatcaa agggggaaat catttgattt ttaactacct
    14461 ttccacctat tttcaggtct gtcagggtaa tgaaataagg tgttgggtta ctgaccgtaa
    14521 ccatgtcagc tgacgaagtg caacgcagtt tcgccggagc ctcgcctaag ggatacgcta
    14581 aaccttttgg tcgccagaaa agtttgatga cgctttgtgt ggcaatcttc agggtatttc
    14641 cctcttcccg cttcgttgtt gaagggactg cccgtacgct agtgaagaaa agggtttctc
    14701 tgtctttcgc caacggcgca ccaatgtaca taatgcgtaa cagattctca ttagccggat
    14761 tcagcacaaa tagcggtggt gtaataataa aatctgctga ttttttatta tctggggtca
    14821 tcacccatga ctgaataaga taaggaacat ccggactggt gttacgtaca cccagtgaaa
    14881 tttgctcttc accttctgaa taaacaagac gtgttgctgc aagtccaatc ccgcctttac
    14941 ctgccgccca tgcggatgag cagctgagta atattgccgt taaacttacg gtcacaattc
    15001 cctttgttat gcaagttttc ataaatagtc actcttaaaa aatatgaata tcccctccag
    15061 aacactagag gggtactaat taataaaaca attaatgagt tctattaata atagttaata
    15121 tggaaatttg ttaccgcatt cacattacca gaagtcgctt tccccgtagc gatataatca
    15181 actttaaagg tcactgtttg ctcagcagaa tccgctaact gaatattcga cgtttcggta
    15241 ccgggagtta ccgatgtaga accatctggc agatacattt gtagagcaac attcgtcgct
    15301 gcatcggtgg tagcctggtt ggcaaatgct gtaggttgca cgccgtcagc cgtaccacta
    15361 aaggtaaacg tcgcattttt agttacagta acatcacagc cttctaaggc aattggtaca
    15421 tcagttttaa cgccagagac tgctccatta gtttgcaact tggtggcgct gacatttggc
    15481 aatgtgacta ctgaatcttt cgtcgcagct accagggtac aagctggagc aataacttta
    15541 ccattaaatg ttactttgcc atccgtgata gcattatcat cagctgccat aacgctggac
    15601 gttgcacata ccacagttat aaacgccgtc aatacacttt ttttcatcgt catcatcctg
    15661 atctcttcgt aaatatgata atgcctttaa atgacattag acttaataat aacaataatt
    15721 gaccgccatt taaatcaagc atactaaatc caataagtga caaatcttta atttatagat
    15781 ttcatgaata tttaaatatt aactgaacga gtaattcatt gattcaacat ctaatgacta
    15841 tccaacaaat aaatatgcgt gatatataaa gccctttctt tagtttattt tcagaaaaag
    15901 atacacaaaa tttatatttg gcattttctt gtctctccga cagactgacc gaattgttat
    15961 caatgttaac aaaaaaagaa caattggtta taaggagaga gtatgcgtgt catcaccctg
    16021 gcgggtagtc ctcgctttcc ttctcgctcc agctccttgc tggaatatgc gcgggaaaaa
    16081 ctaaatggcc tcgatgtaga ggtttatcac tggaatctgc aaaacttcgc cccggaagat
    16141 ctgctttatg ctcgtttcga tagtccggca ctcaagacct tcaccgaaca gctgcaacag
    16201 gctgatggac tgatagtcgc tacacctgtg tataaagccg cctattccgg cgcgctgaaa
    16261 accctgctcg acctgctgcc agagcgcgct ttgcaaggca aagtggtgct accgctggcg
    16321 acgggcggta ccgtggccca tctgctggcg gtcgattatg cccttaaacc agttttaagc
    16381 gcactgaaag ctcaggagat cctgcacggc gtgtttgccg atgactcaca agtaattgat
    16441 taccatcaca gaccccagtt cacgccaaat ctgcaaaccc gtcttgatac cgcgctagaa
    16501 actttctggc aggcattgca ccgccgcgat gttcaggttc ctgaccttct gtctctgcga
    16561 ggtaatgccc atgcgtaaca tcattaaact ggcgctggtg ggattgctta gcgtctctac
    16621 gattgcggtt gctgcagaat cctcgcctga agcgttacgt ataggctatc agaaaggcag
    16681 tattggtatg gtactggcaa aaagccacca gttactggaa aaacgctatc cgcaatcaaa
    16741 aatctcctgg gtggagttcc ccgcgggtcc gcaaatgttg gaagcgttaa acgttggcag
    16801 tattgatctc ggcagtaccg gggatattcc gccaatcttt gcccaggctg ccggggctga
    16861 tttggtatat gtgggcgtcg agccaccgaa gcccaaagcc gaagtgattc tggtggcaga
    16921 aaacagcccg atcaaaaccg tagccgatct taaaggtcac aaagttgcct ttcagaaagg
    16981 ttccagttca cacaaccttt tactgcgtgc actgcgtcag gccggactta agtttaccga
    17041 tatccaaccc acttacctga cgcccgctga tgcccgcgcc gcgttccagc aaggtaacgt
    17101 tgacgcctgg gctatctggg atccctacta ctctgctgca ttattacagg gcggcgtgcg
    17161 ggtgctgaaa gacggcaccg atctcaatca aactggatcg ttttatctgg cagctcgtcc
    17221 ctatgcagaa aaaaacggcg cttttattca gggcgtactc gcaaccttta gtgaggccga
    17281 tgcgttaacc cgcagccagc gcgagcaaag catcgcttta ctggcaaaaa cgatgggctt
    17341 accggcaccg gtgattgcct cttacttaga tcatcgccct cctaccacca tcaaaacggt
    17401 taacgccgag gttgccgcct tacagcagca aacggcagat ctgttttatg aaaatcgtct
    17461 ggtgccgaaa aaagtcgata ttcgccagcg catctggcag cccactcaac tggaaggaaa
    17521 acaattatga gtctgaatat gttctggttt ttaccgaccc atggtgacgg gcattatctt
    17581 ggaacggaag aaggttcacg cccggttgat cacggctatc tgcaacaaat tgcgcaagcg
    17641 gcggatcgtc tgggctatac cggtgtgctg atcccaacgg ggcgatcgtg tgaagatgcg
    17701 tggctggtgg cggcgtcgat gatcccggtg acgcagcggc tgaagtttct tgtcgccctg
    17761 cgccccagcg taacctcacc taccgttgcc gctcgccagg ccgccacgct tgaccgtctc
    17821 tcaaatggtc gtgcgttgtt taatctggtc acaggcagcg atccacaaga gctggcaggc
    17881 gacggcgtgt tccttgatca tagcgagcgc tacgaagcct cggcggaatt tacccaggtc
    17941 tggcggcgct tattgctggg ggaaaccgtc aatttcaacg gtaaacatat tcatgtgcgc
    18001 ggagcaaaac tgctcttccc gccgattcaa cagccttatc ctccgcttta ctttggcgga
    18061 tcgtcagacg ttgcccagga gctggcggca gaacaagttg atctctacct cacctggggc
    18121 gaaccgccag aactagtaaa agagaagatc gaacaagtgc gggcgaaagc tgccgcgcat
    18181 ggacgcaaaa ttcgtttcgg tattcgtctg catgtgattg ttcgtgaaac taacgacgaa
    18241 gcgtggcagg ccgccgagcg gttaatctcg catcttgatg atgaaactat cgccaaagca
    18301 caggccgcat tcgcccggac ggattccgta ggacaacagc gaatggcggc gttacataac
    18361 ggcaagcgcg acaatctgga gatcagcccc aatttatggg cgggcgttgg cttagtgcgc
    18421 ggcggtgccg gaacggcgct ggtgggcgat ggtcctacgg tcgctgcgcg aatcaacgaa
    18481 tacgccgcgc ttggcatcga cagctttgtg ctttcgggct atccgcatct ggaagaagcg
    18541 tatcgggtcg gcgagttgct gttcccacat ctggatgtcg ccattccgga aattccccag
    18601 ccgcagccgc tgaatccgca aggcgaagcg gtggcgaatg attttatccc ccgtaaagtc
    18661 gcgcaaagct aaggaagaaa taatggcaac gccagtgaag aagtggttat tgcgcgttgc
    18721 cccctggttt ttaccggtgg gcatcgtggc ggtgtggcaa ctggcctcct cagttggctg
    18781 gctgtcgacg cgtattttgc cctcaccgga aggggtggtg acggcgttct ggacgctctc
    18841 cgccagcggc gaactgtggc agcatctggc gatcagttcc tggcgggcgc tgattggctt
    18901 ttcaattggc ggatcgctgg ggttgatttt gggactgatt agcgggctat ctcgctgggg
    18961 agaacggctg ctggatacct caattcagat gttgcgcaac gtaccgcatc tggcgctgat
    19021 cccgctggtg attttgtggt ttggcattga tgaatccgca aaaatctttc tggtggcgct
    19081 cggcacgcta ttccccattt atatcaacac ctggcatggc atccgtaata tcgatcgcgg
    19141 tctggtggaa atggcgcgta gctatggatt atccggcata ccgctgttta tccatgtgat
    19201 cctgcctggt gccctgccct caattatggt cggcgtgcgt tttgcgttgg gcctgatgtg
    19261 gctgacgctg attgttgccg aaaccatttc tgccaattca ggcattggtt atctggcgat
    19321 gaatgcgcgg gagtttttgc aaacggacgt ggtggtagtc gctattattc tttacgccct
    19381 gctcggcaaa cttgctgacg tcagcgcgca gctgttagag cgcctctggt tgcgctggaa
    19441 cccggcttat catttgaagg aggccacggt atgaatactg ctcgtctgaa ccagggcaca
    19501 ccattgttgc tcaatgcagt aagcaaacat tacgcggaaa atatcgtcct gaaccaactg
    19561 gatttacata ttccggcagg tcagtttgtg gcggtggtgg gccgcagcgg tggtggcaaa
    19621 agtaccctgc tgcgcctgct ggcaggtctg gaaacgccaa ccgcaggcga tgtgttagcg
    19681 ggcaccacac cgctggctga gattcaggaa gacacgcgaa tgatgtttca ggatgcgcgt
    19741 ctgctgccgt ggaaatcggt gattgataac gttgggttag gccttaaagg ccagtggcgc
    19801 gatgccgcgc gtcgggcgtt ggctgcagta ggactggaga atcgcgccgg ggaatggcct
    19861 gccgcacttt ctggcgggca gaaacagcgt gtggcgctgg caagagcgtt gattcatcga
    19921 ccgggtttat tgttgcttga tgaaccgctc ggggcgctgg acgccttaac gcgactcgag
    19981 atgcaggatt tgattgtgtc actttggcag cagcacggct ttaccgtact gttggtgacg
    20041 catgatgtga gcgaagcggt agcaatggct gaccgggtgt tgttaattga agagggaaaa
    20101 attggtctgg atttgacagt ggatatcccc cgtccgcgtc gattagggtc ggtgcgatta
    20161 gcggagctgg aagcggaagt attgcaacgg gtaatgcggc gtgggcattc tgaacaactg
    20221 attcggcgtc atggttaata tgccggatgt ggcgcacaaa accagcattt tcaacggact
    20281 agtaggcctg ataagacacg tcaagcatcg catcaggcag ttgggcacgg ctgccggatg
    20341 cggcgtgaac gccttatcca gcctacaaaa ccagcaattt caacggagtt gtaggcctga
    20401 taagacgcgt caagcgtcgc atcaggcatt ttggctcaaa agtatcaagc cagtgctttg
    20461 gtgatcttct cgtacagatc gccagagaga ttttccagcc ctttcaactg ttccagcgcc
    20521 gcgcgcattt tctcctgacg tttggcatca taacgtttca ggcgaatcag cggttcaatc
    20581 agacgtgaag ctacctgcgg gttacggctg ttgaggtcgg taagcatttc caccaggaac
    20641 tggtaaccgc tgccatcttc ggcatggaac gctgccgggt tgctgcccgc aaacgcacca
    20701 atcaacgaac gaatacggtt cgggttgctc atggtaaatg agcgatgctg caacagacca
    20761 cgcaccgtct ccagcacatt cgccgccggg ctggtggctt gcaggataaa ccatttatcc
    20821 atcaccagac cgtcctgatg ccacttatcg tcgtactcct gcatcagcgc gtcacggcaa
    20881 ggcagctgtg cagcaaccgc cgcagaaagc gctgccagcg catcggtcat attgtttgct
    20941 tcatggtatt gcttgctcac cagcacgtca gccagatgcg tttcaccaaa agcaaggaag
    21001 cgcaggcagg cattacgcag agtgcgtttt gcaatatctt catgctcaac acggtactcg
    21061 ctctggtaat tcgcgttgta aatagccagc agctcatccg ccagttcagt cgccagagta
    21121 cgagtgagtg cttcgcgtac ttcggcaata gcaatcgggt cgatgatatc gaacagttca
    21181 gccatttcat tgacagaagg cagcgtcagg atttctgccg ccagcgccgg atcaatcttc
    21241 tcatcgagca gcaccgcgcg gaaagcgtca gccacatgta ccggcagaga cagcggctgc
    21301 ccttgctgat gacgggcgac gttcagcttg atgtaggttg ccagcaaact ttgcgccgca
    21361 tcccaacggg agaaatcatt acgcgcatga cgcatcagga aggtcagttg ctgatcgctc
    21421 cacttatatt ccagtttcac tggcgcagag aattcgcaca gcagcgcagg caccggctgg
    21481 aagtagacat tatcaaaaac aaaggtctgt tccgcctggg tgacgttcag cacggaattc
    21541 accggatgac cgcctttctg caacgggatc actttgcctt cgttgtcata caattcgatg
    21601 gcaaatggaa tatgcagcgg ctgtttttct gcctgatccg gcgtggctgg cgtgcgctgg
    21661 ctgatggtca gggtgtactg ctcggtttcc ggattgtagt cgtctttgac ggtcacaatc
    21721 ggcgtgccgg actggctgta ccaacggcgg aaatgggaga gatcgacatt cgacgcatct
    21781 tccatcgcct gcacaaagtc gtcgcaggtt gccgcgctgc catcatggcg ctcgaaatag
    21841 agttgcatcc ctttctggaa gttttcttcg cccagcaggg tgtggatcat gcgaatcact
    21901 tccgcgccct tctcgtaaac ggtcagggtg tagaagttgt tcatctcaat gaccatatcc
    21961 gggcggatcg ggtgcgccat cgggctggcg tcttccgcaa actgcaatcc gcgcatggtg
    22021 cgcacattat tgatacggtt taccgcgcgg gaaccaaggt cagagctgaa ttcctgatcg
    22081 cggaagacgg ttaaaccttc tttcaggctg agctggaacc agtcgcggca ggtgactcgg
    22141 ttaccggtcc agttatggaa atattcatgg ccgataacgc gttcaatatc gaggtaatct
    22201 ttgtcagtgg cggtgtcggt gcgggccagc acatatttgg agttaaagat attcaaaccc
    22261 ttattctcca ttgcgcccat attgaagaaa tccaccgcga cgatcatata gatgtcgagg
    22321 tcatactcca ggccgaagcg ttcttcatcc catttcattg agtttttcag cgaggtcatc
    22381 gcccacggcg cgcgatcaag gttgccgcga tcgacgtaca gctccagcgc cacttcgcga
    22441 ccagaacgcg tggtaaaggt gtcgcgcagt acatcaaagt cgcctgccac cagcgcaaac
    22501 aggtagcacg gtttcgggaa cgggtcctgc cactgtaccc aatggcgtcc gttttccagt
    22561 tcaccttgcg caacgcggtt accgttggaa agcaggaagg gatatttggt tttatcggca
    22621 ataattttgg tggtaaaacg cgccagcacg tccgggcggt cgagataata cgtaatatgg
    22681 cggaaacctt cggcttcaca ctgggtgcaa agcgcatcgc ctgactgata aagcccttcc
    22741 agcgccgtat tcgccgccgg gcttatttca ttaatgatct taagcgtaaa acgctccggc
    22801 aaattactga tgaccagtgc gccctcttct tctttccagg cggtccacgg ctcatcatta
    22861 atatgaacag aaaccagttt gaggtcttcg ccgttgagac gaagcggagc atctgatgca
    22921 ccatgacgga cagcctggct gaccgcggta acgaccgtct tttgcgcgtc gaggtcaaag
    22981 gtcaagtcaa tatcagtaat ctggtaatcc ggcgcacgat aatcgtgacg gtatttggct
    23041 tgtggctgtt gagtcataaa taacctttag catcttttat agagtctggt gttcagtcta
    23101 ttcctgttgc gtaaatcgcg ctatgcagaa tcttcatctt ttcaggtaca aacgccttta
    23161 ttgctacatt tttataacat acaccgcgta atgccatcga ccagaaaggt ggcatatggt
    23221 gtgatcgggg ttcaataaat tgcgaaacaa ggtatactcc agcagttcct gaagatgttt
    23281 attgtactaa acgctcctgt acgaggacgc tactgcgcac ctatgacaca attcgcttct
    23341 cctgttctgc actcgttgct ggatacagat gcttataagt tgcatatgca gcaagccgtg
    23401 tttcatcact attacgatgt gcatgtcgcg gcggagtttc gttgccgagg tgacgatctg
    23461 ctgggtattt atgccgatgc tattcgtgaa cagattcagg cgatgcagca cctgcgcctg
    23521 caggatgatg aatatcagtg gctctctgcc ctgcctttct ttaaggccga ctatcttaac
    23581 tggttacgcg agttccgctt taacccggaa caagtcaccg tgtccaacga taatggcaag
    23641 ctggatattc gtttaagcgg tccgtggcgt gaagtcatcc tctgggaagt tcctttgctg
    23701 gcggttatca gtgaaatggt acatcgctat cgctcaccgc aggccgacgt tgcgcaagcc
    23761 ctcgacacgc tggaaagcaa attagtcgac ttctcggcgt taaccgccgg tcttgatatg
    23821 tcgcgcttcc atctgatgga ttttggcacc cgtcgccgtt tttctcgcga agtacaagaa
    23881 accatcgtta agcgtctgca acaggaatcc tggtttgtgg gcaccagcaa ctacgatctg
    23941 gcgcgtcggc tttccctcac gccgatggga acacaggcac acgaatggtt ccaggcacat
    24001 cagcaaatca gcccggatct agccaacagc cagcgagctg cacttgctgc ctggctggaa
    24061 gagtatcccg accaacttgg cattgcatta accgactgca tcactatgga tgctttcctg
    24121 cgtgatttcg gtgtcgagtt cgctagtcgg tatcagggcc tgcgtcatga ctctggcgac
    24181 ccggttgaat ggggtgaaaa agccattgca cattatgaaa agctgggaat tgatccacag
    24241 agtaaaactc tggttttctc tgacaatctg gatttacgca aagcggttga gctataccgc
    24301 cacttctctt cccgcgtgca attaagtttt ggtattggga ctcgcctgac ctgcgatatc
    24361 ccccaggtaa aacccctgaa tattgtgatt aagttggttg agtgtaacgg taaaccggtg
    24421 gcgaaacttt ctgacagccc tggcaaaact atctgccacg ataaagcgtt tgttcgggcg
    24481 ctgcgcaaag cgttcgacct tccgcatatt aaaaaagcca gttaatatca tcagggagct
    24541 aatcggctcc ctttttttac ctttaattcc gaaatctttc gctgcatttg cgaattctgc
    24601 ttgtctgatt gcagatgcca ggtaacatag gtatcccccc atttacgggt ggacaagtgt
    24661 ttattttttc cgactattaa cagagagaat attatgagcg ttgtgcctgt agccgacgta
    24721 ctccagggcc gcgtagccgt tgacagcgaa gtcaccgtac gcggatgggt acgtacccgc
    24781 cgagattcaa aagctggcat ctccttcctc gccgtttatg acggttcctg ctttgatcct
    24841 gtacaggctg tcatcaataa ttctctgccc aattacaatg aagacgtcct gcgtctgacc
    24901 accggctgct cggtcattgt gacgggtaaa gtcgtggcgt cgccgggcca ggggcaacaa
    24961 tttgaaattc aggccagcaa agttgaagtt gctggttggg ttgaagatcc agacacttac
    25021 ccgatggcgg caaaacgcca cagcattgag tatctgcgtg aagtcgctca cctgcgtccg
    25081 cgcacaaacc tgattggtgc cgtcgcgcgc gttcgccata cgctggcgca ggcgctgcat
    25141 cgcttcttta acgagcaggg attcttctgg gtttcaacgc cactgattac cgcatctgat
    25201 accgaaggtg caggcgaaat gttccgcgtt tctacgctgg atctggaaaa cctgccgcgt
    25261 aacgatcagg gcaaagtgga tttcgacaaa gacttctttg gtaaagagtc tttcctgacc
    25321 gtatctggcc agttgaacgg cgaaacctac gcttgcgcat tatccaaaat ttataccttc
    25381 ggcccgactt tccgtgctga aaactccaac accagccgtc acctggcgga attctggatg
    25441 ctggagccgg aagtggcgtt tgctaacctg aacgatatcg cgggtctggc tgaagccatg
    25501 ctgaaatatg tcttcaaagc ggttctcgaa gaacgcgcag acgacatgaa attcttcgct
    25561 gaacgcgtag ataaagatgc cgtttcacgt ctggaacgct tcattgaggc cgatttcgcg
    25621 caggtggatt acaccgacgc agtgaccatt ctcgaaaact gcggcaggaa gtttgaaaac
    25681 ccggtttact ggggcgtcga tctctcttct gagcatgagc gttatctggc ggaagaacac
    25741 tttaaagcac cggtagtggt taaaaactat ccgaaagata ttaaagcgtt ctatatgcgc
    25801 ctcaacgaag acggtaaaac cgttgcggct atggacgttc tggctccggg catcggtgag
    25861 atcattggtg gctcccagcg tgaagaacgt ctggacgttc tggacgagcg tatgctggaa
    25921 atgggcctga ataaagaaga ttactggtgg tatcgcgatc tgcgtcgcta cggtactgtt
    25981 ccgcattcag gtttcggtct tggttttgaa cgcctgattg cttacgtaac tggcgtgcaa
    26041 aacgtgcgtg atgtgattcc gttcccacgt actccgcgca acgccagctt ctgattttct
    26101 tcctcaaaga gccagcgcct gctggctctt ttttatcctt tctgtcaagt tatctgtttg
    26161 ttaagtcaag caatctattt gcaaccccgc cataacgatc atcctgttac ggaatattac
    26221 attgcaacat ttacgcacaa aaaataaccc gcattcttat tgcggattag ttttttctta
    26281 gctgatagca caattttcat actatttttt ggcattctgg atgtctgaaa gaggattttg
    26341 tgccaggtcg ataaagtttc catcag
//
LOCUS       DAMZET010000050        26364 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.050, whole genome shotgun sequence.
ACCESSION   DAMZET010000050 DAMZET010000000
VERSION     DAMZET010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.050"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1203
                     /gene="rcsD"
                     /locus_tag="POB34_002150"
     CDS             <1..1203
                     /gene="rcsD"
                     /locus_tag="POB34_002150"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HDI5919093.1"
                     /translation="ESKQLANQADVLVRLVDEIQLANMLADDSWKSETVLFSVQDLID
                     EVVPSVLPAIKRKGLQLLINNHLKAHDMRRGDRDALRRILLLLMQYAVTSTQLGKITL
                     EVDQDESSEDRLTFRILDTGEGVSIHEMDNLHFPFINQTQNDRYGKADPLAFWLSDQL
                     ARKLGGHLNIKTRDGLGTRYSVHIKMLAADPEVEEEEERLLDDVCVMVDVTSAEIRNI
                     VTRQLENWGATCITPDERLISQDYDIFLTDNPSNLTASGLLLSDDESGVREIGPGQLC
                     VNFNMSNAMQEAVLQLIEVQLAQEEVTESPLGGDENAQLHASGYYALFVDTVPDDVKR
                     LYTEAATSDFAALAQTAHRLKGVFAMLNLVPGKQLCETLEHLIREKDVPGIEKYISDI
                     DSYVKSLL"
     gene            1220..1870
                     /gene="rcsB"
                     /locus_tag="POB34_002151"
     CDS             1220..1870
                     /gene="rcsB"
                     /locus_tag="POB34_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HDI5919094.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(2070..4919)
                     /gene="rcsC"
                     /locus_tag="POB34_002152"
     CDS             complement(2070..4919)
                     /gene="rcsC"
                     /locus_tag="POB34_002152"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HDI5919095.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            5086..6912
                     /gene="atoS"
                     /locus_tag="POB34_002153"
     CDS             5086..6912
                     /gene="atoS"
                     /locus_tag="POB34_002153"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HDI5919096.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            6909..8294
                     /gene="atoC"
                     /locus_tag="POB34_002154"
     CDS             6909..8294
                     /gene="atoC"
                     /locus_tag="POB34_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HDI5919097.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            8490..9152
                     /gene="atoD"
                     /locus_tag="POB34_002155"
     CDS             8490..9152
                     /gene="atoD"
                     /locus_tag="POB34_002155"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HDI5919098.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            9152..9802
                     /gene="atoA"
                     /locus_tag="POB34_002156"
     CDS             9152..9802
                     /gene="atoA"
                     /locus_tag="POB34_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HDI5919099.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            9799..11121
                     /locus_tag="POB34_002157"
     CDS             9799..11121
                     /locus_tag="POB34_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HDI5919100.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            11152..12336
                     /gene="atoB"
                     /locus_tag="POB34_002158"
     CDS             11152..12336
                     /gene="atoB"
                     /locus_tag="POB34_002158"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HDI5919101.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIGLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     PEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(12410..13186)
                     /locus_tag="POB34_002159"
     CDS             complement(12410..13186)
                     /locus_tag="POB34_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HDI5919102.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(13191..14840)
                     /locus_tag="POB34_002160"
     CDS             complement(13191..14840)
                     /locus_tag="POB34_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HDI5919103.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLPTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKSKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(14841..19355)
                     /locus_tag="POB34_002161"
     CDS             complement(14841..19355)
                     /locus_tag="POB34_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HDI5919104.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPFEPQQGVKLEGVSSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGKELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     IGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVTLNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVRVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTMYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQAIGVTQQPEYWQHMLDSYAEQADNVPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDPSDINDSLIFDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            complement(19379..20002)
                     /locus_tag="POB34_002162"
     CDS             complement(19379..20002)
                     /locus_tag="POB34_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HDI5919105.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANEALKVHDSKWLKSNGIASQYLPPEMTLKPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWILNDSNPNFIGIYRLNFLAR"
     gene            complement(19999..21687)
                     /locus_tag="POB34_002163"
     CDS             complement(19999..21687)
                     /locus_tag="POB34_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HDI5919106.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERAAEKTPVRQRIVASARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGIAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(21836..24463)
                     /gene="gyrA"
                     /locus_tag="POB34_002164"
     CDS             complement(21836..24463)
                     /gene="gyrA"
                     /locus_tag="POB34_002164"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HDI5919107.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            24610..25332
                     /locus_tag="POB34_002165"
     CDS             24610..25332
                     /locus_tag="POB34_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HDI5919108.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            complement(25460..>26364)
                     /locus_tag="POB34_002166"
     CDS             complement(25460..>26364)
                     /locus_tag="POB34_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDI5919109.1"
                     /translation="QPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGG
                     DYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGTDGEWMLGIVGGYSDNQGDSRSNMTGT
                     RADNQNHGYAVGLTSSWFQHGNQKQGAWLDSWLQYAWFSNDVSEQEDGTDHYHSSGII
                     ASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANRARVSQSQGDDIQTRLGLHSE
                     WRTAVHVIPTLDLNYYHDPHSTEIEEDGSTISDDAVKQRGEIKVGVTGNISQRVSLRG
                     SVAWQKGSDDFAQTAGFLSMTVKW"
BASE COUNT         6281 a         7085 c         6598 g         6400 t
ORIGIN      
        1 gaaagcaaac aactggcgaa tcaggcagat gtgctggtgc ggctggtcga tgaaatacag
       61 ttagcgaaca tgcttgcgga cgatagctgg aaaagtgaga cggtgctgtt ctccgtgcag
      121 gatttaattg atgaagttgt gccttcagtg ttgcctgcca tcaagcgtaa aggtctgcaa
      181 ctgctgatta acaatcatct gaaagcacac gatatgcgcc gtggcgatcg cgatgcctta
      241 cgacgtattt tgctgctact gatgcaatat gccgtgacct caacgcaatt gggaaaaatc
      301 acccttgagg ttgatcagga tgagtcctcc gaagaccgcc tgacgtttcg cattctggac
      361 acgggagaag gcgtaagtat tcatgaaatg gataatttgc acttcccgtt tatcaaccag
      421 acccaaaacg atcgttatgg aaaggcggac ccgctggcat tctggctgag cgatcaactg
      481 gcacgtaaac tgggcggtca tttaaacatc aaaacgcggg atgggcttgg tacacgctac
      541 tctgtgcata tcaaaatgct cgccgctgac ccggaagttg aagaggaaga agagcgttta
      601 ctggatgatg tctgcgtaat ggtggatgtt acttcggcag aaattcggaa tattgtcact
      661 cgccagttag aaaattgggg tgcaacctgt atcacacccg atgaaagatt aattagtcaa
      721 gattatgata tctttttaac ggataatccg tctaatctta ctgcctctgg cttgctttta
      781 agcgatgatg agtctggcgt acgggaaatt gggcctggtc aattgtgcgt caacttcaat
      841 atgagcaacg ctatgcagga agcggtctta caattaattg aagtgcaact ggcgcaggaa
      901 gaggtgacag aatcgcctct gggcggagat gaaaatgcgc aactccatgc cagcggctat
      961 tatgcgctct ttgtagacac agtaccggat gatgttaaga ggctgtatac tgaagcagca
     1021 accagtgact ttgctgcgtt agcccaaacg gctcatcgtc ttaaaggcgt atttgccatg
     1081 ctaaatctgg tacccggcaa gcagttatgt gaaacgctgg aacatctgat tcgtgagaag
     1141 gatgttccag gaatagaaaa atacatcagc gacattgaca gttatgtcaa gagcttgctg
     1201 tagcaaggta gcctattaca tgaacaatat gaacgtaatt attgccgatg accatccgat
     1261 agtcttgttc ggtattcgca aatcacttga gcaaattgag tgggtgaatg ttgtcggcga
     1321 atttgaagac tctacagcac tgatcaacaa cctgccgaaa ctggatgcgc atgtgttgat
     1381 taccgatctc tccatgcctg gcgataagta cggcgatggc attaccttaa tcaagtacat
     1441 caagcgccat ttcccaagcc tgtcgatcat tgttctgact atgaacaaca acccggcgat
     1501 tcttagtgcg gtattggatc tggatatcga agggatcgtg ctgaaacaag gtgcaccgac
     1561 cgatctgccg aaagctctcg ccgcgctgca gaaagggaag aaatttaccc cggaaagcgt
     1621 ttctcgcctg ttggaaaaaa tcagtgctgg tggttacggt gacaagcgtc tctcgccaaa
     1681 agagagtgaa gttctgcgcc tgtttgcgga aggcttcctg gtgaccgaga tcgctaaaaa
     1741 gctgaaccgc agtattaaaa ccatcagtag ccagaagaaa tctgcgatga tgaagctggg
     1801 tgtcgagaac gatatcgccc tgctgaatta tctctcttct gtgaccttaa gtccggcaga
     1861 taaagactaa tcatctgtag gccagataag acgcgttagt gtcttatctg gcatttgcac
     1921 cgattgccgg atgcagcgta aacgccttat ccggcctacg attcccatta tttcaacaga
     1981 ttacattaaa gtaggccaga taagacgcgt cagcgtcgca tctggcattt gcactgaatg
     2041 ccagatgcgg cataaacacc ttatccgtcc tacgattccc gcgatttcct gaccctctcg
     2101 gcatataccg tcagcgtctg ttttatcaca tccagcgtta ccggcttcga caggcagctg
     2161 tccataccgg actccagaca ccgctgcttc tcttcagcca acgcattagc agttactccg
     2221 attaccggca acgtcagtcc caactgacga atgcgttgcg tcaagcggta accatccata
     2281 tttggcatgt tgacgtcgct aagcacgata tcaatatgat tcttgctaag tacattaagc
     2341 gcatcgacac catcattcgc ggttttacat tgatagccca acgatcccaa ctgatctgcc
     2401 agcagacgtc ggttaatcgg atgatcatcc acgaccagaa tcatcatatc gtcattatcg
     2461 ctgaccgctt tgtccgtcga cggcagagcg ttagcaggat cgtcgctctc catctcgatc
     2521 aaataaatac gcgccaacaa tgccggtagc tcatgcgggg cagccacact gtgtacccac
     2581 tcccctggcg ctttctccag cggaatacca atatggcgac gacagaaggt cactaccgct
     2641 ctaccctgcc attttttact cactacctcg tcagtaatta acacatcttc gggagtcggt
     2701 tcctgccctt cgtatgttgt aacgacgatg ccgctgcgct gcaaactggt ttccaggaac
     2761 tgacagagcg acgcattgcg gaccgccagc cagcagcgtt taccactcaa cccttccacg
     2821 ccttttttct gcgggtactg agcgccgtac aacggaatac gcacggtaaa ctggctgccc
     2881 attcccggtt ctgaatctac cgagatatcg ccgtccatca tgctgatcag tttttcacaa
     2941 atcgccagac ccagaccggt cccctggaag tttcgctgta cgcccgttcc gacctggaag
     3001 aagggatcaa acaagcgcac cacttctttc gccggaatcc ccacgccggt atcgcgaaca
     3061 cggatagaga gataatcgcc atcagcgcgg acatgcaaaa ctatacagcc ggtatcggtg
     3121 aattttatgg cgttactcaa caggttggag atgacctgct gtaaacgcat cgggtcgcca
     3181 tttaaggcca ctggcacatc cggttcaata aagcagtaca agcctagctg cttgcgtacc
     3241 accagcggta aatagttggc ggtgatgtgg ttcatcactt cacgcggtga aaactcacgc
     3301 ggttcgatct tcaactgttc cgattcaatc ttcgagaaat cgagaatatc gctgataatt
     3361 ttcaacaaca ggctggaaga gttgttcatt gccgtcacca gccgatcgac gcctttcggt
     3421 aactctttgg tttgcaacag atccaggtta ccgataatgc catacagcgg cgttcgcagc
     3481 tcatgactga cggtggcaag gaacatcgat tttgactggc tcgcctgttc cgctgcttgt
     3541 gccatctcct gcaacgactc ttccatcttc acgcgcgaag aaacatccac cagcacacaa
     3601 atggccacgt tttcattacg atagcgcgaa tggacgaagc tgatttgcag attggtattg
     3661 ttgctggtca ggacatcaac aaaattgacc tgctgcccac agataatttg cgtcagtcgt
     3721 tggcggtcct catgcgtaag catattgaga taggtatgcg ccagttcgtt acttaaaata
     3781 ttgacgccat cagcggtacg caaaatgcag atacccactg gcgcggaggc gacaatcttg
     3841 cgattgaact gctcatgttc ttccagtcgc agggcgtcgc tttccgccgg aatgaaaata
     3901 cgtcgctcgt acatccgtgc gagagtaaac aatgcagctc cggcaagcac attcagcaaa
     3961 attgcgttaa ggatcaacat gcgaatgcgt tccagcacct tatcaaccgg caccgaatac
     4021 acgatgctta gcgatgaggg tggcagattt ttcttcagca ccagctcccg gaacccttcc
     4081 gtatagccaa accaggagcg ttcctgcatc cagcgaggat cgcccttaat tttactttct
     4141 ggtccggtaa gcgaaatcag ggtatgacca ttttcatcaa gaatggtaac ccccatcggc
     4201 aacgtacccg gtaagaaaaa gttctccatc cggatggtct gctcgacacc caaaagcgcc
     4261 tgcaaccggt tcgccagata aactggcgtc aacgcgtaaa aatacccgac gccagggcgc
     4321 ggaccttcgc tgatccagta gaggttactg ccgctatcat cttgtggtgc atttcgatat
     4381 ttattgatgc gttcatgcaa agctttcaac gcggtatcgc gttccactgg catatcacgc
     4441 agaccgaaat tggccatgca gaggttatcg ctgccgatta aaaatacccg gtttagatcg
     4501 taagccgcag aaaaattatc gcgccagtag cgcataaacc acgccaatga ctccagagaa
     4561 cctcgccagg tgttactcat tgcggaacaa tcggagtcgg caaacagcgg ttcaaacgca
     4621 ggcacatccg cctgcgtttc tcgtccacgc ggggaaagca caccgttttc tgccgataag
     4681 cgattttcgg cgatgtactt cagctctttc atcacatcag aggtgcgttg aataaagcgc
     4741 tgagcctgat cggaactcag attaaattcc tgacgaattt ccgattctcg ctgatgtaac
     4801 gcattaacga tgtaaaaaac ggatgaaaaa gcaatcaaca gccagagcac taacgccaat
     4861 gctctgaaca tgtagcgcga ggctttcagg gttgtacgaa aagaagcaag gtatttcaaa
     4921 ggggcgaagc tccgcctcag gtgaccgatg gagtgtggtt aaggtagcgg taaaagcgtg
     4981 ttaccgcaat gttctctctt ctctggaata tgatacaccg ccgagaaatc atcaccttaa
     5041 cctctgataa tcgtcatata ccggacaaga ctagtggatt tcagcatgca ttatatgaag
     5101 tggatttatc cacgccgctt acgcaatcaa atgatcctga tggcaatcct gatggtcatt
     5161 gtcccaacgc ttactattgg ttatatcgta gaaacggaag gacgttcagc agtcttatct
     5221 gaaaaagaga aaaaactttc tgccgtggtc aacctgctta atcaggcact aggcgatcgc
     5281 tatgatctct acatcgactt accacgtgag gagcgtatcc gcgcattaaa tgcagaactt
     5341 gcccccatta ccgaaaatat cactcacgcc ttccctggca tcggtgctgg ttattacaac
     5401 aaaatgctgg atgcgataat cacctacgcg ccttcagcgc tatatcagaa taatgtcggc
     5461 gttaccattg ccgcagatca ccctggtcgc gaagtcatgc gtacaaatac ccctttggtt
     5521 tattcaggca ggcaggtgcg cggcgatatt ttgaattcaa tgctccccat tgagcgtaat
     5581 ggtgaaatcc tcggctatat ctgggccaat gaattaaccg aagatattcg ccgccaggcc
     5641 tggaaaatgg atgtgaggat tatcattgtg ctcaccgccg gtttgctgat aagcctgcta
     5701 ttgattgtcc ttttctcccg tcgcctgagc gccaatattg atatcatcac cgatggcctc
     5761 tcgactctgg cacaaaatat tcccactcga ttaccacaat tgcccggtga aatggggcaa
     5821 atcagtcaga gtgttaataa cctcgcccag gcactgcgtg aaacgcggac acttaacgat
     5881 ctgattattg aaaacgctgc cgatggcgtc attgccattg accgccaggg tgatgtaacc
     5941 accatgaacc cagcagcaga agttatcact ggctatcaac gccatgaact ggtagggcag
     6001 ccttactcca tgttgttcga caatactcag ttctacagtc cagtactgga tacgctggaa
     6061 catggcaccg aacatgtggc gctggagatc agttttccag gtcgtgaccg caccattgaa
     6121 ctcagtgtca ctaccagtcg tattcataac acgcacggtg aaatgatagg tgctttggtg
     6181 attttctctg atttaactgc ccgcaaagaa acccagcgcc gcatggcgca agcagaacgc
     6241 ctcgccacac tgggtgagct gatggctggc gtcgcgcatg aagtacgtaa tccgttaacg
     6301 gctattcgtg gttatgtaca gatcttgcgc caacaaacca gtgacccaat acatcaggaa
     6361 tatctgtccg tagtactcaa agaaatcgat tcaattaaca aagttattca gcaattgctc
     6421 gaattttcac gtccacgcca cagtcaatgg caacaagtca gcctcaatgc attggttgaa
     6481 gaaactctgg tactggtaca aaccgccggc gtacaagcgc gggtcgactt cataagcgaa
     6541 ctggataatg aattaagccc gattaacgcc gatcgtgaac tgctcaaaca ggtactactg
     6601 aatatcctga tcaatgccgt ccaggctatc agcgcacgag ggaaaattcg cattcaaacc
     6661 tggcaataca gcgactcaca acaggccatt tcgatagagg acaacggctg tggcattgat
     6721 ctctcgctgc aaaaaaagat cttcgatccc tttttcacca ccaaagcctc aggaaccggg
     6781 cttggtctgg cgttaagtca acgcatcatt aatgcccatc agggtgatat tcgcgtcgcc
     6841 agtttgccgg gctacggcgc aaccttcacg cttattttac cgatcaaccc gcagggaaat
     6901 cagactgtat gactgctatt aatcgcatcc ttattgtgga tgatgaagat aatgttcgcc
     6961 gtatgctgag caccgctttt gcactacaag gattcgaaac acattgtgcg aacaacggac
     7021 gcacagcatt acacctgttt gccgatattc accctgatgt ggtgttgatg gatatccgca
     7081 tgccagagat ggacggcatc aaggcactaa aggagatgcg cagccatgag acccggacac
     7141 ccgttattct gatgacggcc tatgcggaag tggaaaccgc cgtcgaagcg ctacgctgcg
     7201 gagccttcga ctatgttatt aaaccgtttg atctcgatga gttgaattta atcgttcagc
     7261 gcgctttaca actccagtca atgaaaaaag agatccgtca tctgcaccag gcactgagca
     7321 ccagctggca atgggggcac attctcacca acagcccggc gatgatggac atctgcaaag
     7381 acaccgccaa aattgccctt tctcaggcca gcgtcttgat tagcggtgaa agcggcaccg
     7441 ggaaagagtt gattgccaga gcgattcact acaattcgcg gcgggcaaag gggccgttca
     7501 ttaaagtcaa ctgcgcggcg ctgccggaat cgttgctcga aagtgaactg tttggtcatg
     7561 aaaaaggtgc atttactggt gcacaaacct tgcgtcaggg attatttgaa cgagccaacg
     7621 aaggtactct gctcctcgac gaaattggcg aaatgccgct ggtactacaa gccaaattac
     7681 tacgcattct acaggaacgg gaatttgaac ggattggcgg ccatcagacc ataaaagttg
     7741 atatccgcat cattgctgcc accaaccgcg acttgcaggc aatggtaaaa gaaggtacct
     7801 tccgtgaaga tctcttttat cgccttaacg ttattcattt aatactgccg cctctgcgcg
     7861 atcgccggga agatatttcc ctgttagcta atcacttttt gcaaaaattc agtagtgaga
     7921 atcagcgcga tattatcgac atcgatccga tggcaatgtc actgcttacc gcctggtcat
     7981 ggccgggaaa tattcgagag ctttccaacg ttattgaacg cgccgtcgtg atgaattcag
     8041 gcccgatcat tttttctgag gatcttccgc cacagattcg tcagccagtc tgtaatgctg
     8101 gcgaggtaaa aacagcccct gtcggtgagc gtaatttaaa agaggaaatt aaacgcgtcg
     8161 aaaaacgcat cattatggaa gtgctggaac aacaagaagg aaaccgaacc cgcactgctt
     8221 taatgctggg catcagtcgc cgtgcattga tgtataaact ccaggaatac ggtatcgatc
     8281 cggcggatgt ataacaccaa aacttgctat gcagaaattt gcacagtgcg caattttctg
     8341 catagccgct cattctcctt ataaatcccc atccaattta tcccttcata ttcaattagt
     8401 taaataacta aatccaataa tctcattctg gcactcccct tgctattgcc tgactgtacc
     8461 cacaacggtg tatgcaagag ggataaaaaa tgaaaacaaa attgatgaca ttacaagacg
     8521 ccaccggctt ctttcgtgac ggcatgacca tcatggtggg cggatttatg gggattggca
     8581 ctccatcccg cctggttgaa gcattactgg aatctggtgt tcgcgacctg acattgatag
     8641 ccaatgatac cgcgtttgtt gataccggca tcggtccgct catcgtcaat ggtcgagtcc
     8701 gcaaagtgat tgcttcacat atcggcacca acccggaaac aggtcggcgc atgatatctg
     8761 gtgagatgga cgtcgttctg gtgccgcaag gtacgctaat cgagcaaatt cgctgtggtg
     8821 gagctggact tggtggtttt ctcaccccaa cgggtgtcgg caccgtcgta gaggaaggca
     8881 aacagacact gacactcgac ggtaaaactt ggctgctcga acgcccactg cgcgccgacc
     8941 tggcgctaat tcgcgctcat cgttgcgaca cacttggcaa cctgacctat caacttagcg
     9001 cccgcaactt taaccccctg atagcccttg cggctgatat cacgctggta gagccagatg
     9061 aactggtcga aaccggcgag ctgcaacctg accatattgt cacccctggt gccgttatcg
     9121 accacatcat cgtttcacag gagagcaaat aatggatgcg aaacaacgta ttgcgcgccg
     9181 tgtggcgcaa gagcttcgtg atggtgacat cgttaactta gggatcggtt tacccacaat
     9241 ggtcgccaat tatttaccgg agggtattca tatcactctg caatcggaaa acggcttcct
     9301 cggtttaggc ccggtcacga cagcgcatcc agatctggtg aacgctggcg ggcaaccgtg
     9361 cggtgtttta cccggtgcag ccatgtttga tagcgccatg tcatttgcgc taatccgtgg
     9421 cggtcatatt gatgcctgcg tgctcggcgg tttgcaagta gacgaagaag caaacctcgc
     9481 gaactgggta gtgcctggga aaatggtgcc cggtatgggt ggcgcgatgg atctggtgac
     9541 cgggtcgcgc aaagtgatca tcgccatgga acattgcgcc aaagatggtt cagcaaaaat
     9601 tttgcgccgc tgcaccatgc cactcactgc gcaacatgcg gtgcatatgc tggttactga
     9661 actggctgtc tttcgtttta ttgacggcaa aatgtggctc accgaaattg ccgacgggtg
     9721 tgatttagcc accgtgcgtg ccaaaacaga agctcggttt gaagtcgccg ccgatctgaa
     9781 tacgcaacgg ggtgatttat gattggtcgc atatcgcgtt ttatgacgcg ttttgtcagc
     9841 cggtggcttc ccgatccact gatctttgcc atgttgctga cattgctaac attcgtgatc
     9901 gcgctttggt taacaccaca aacgccgatc agcatggtga aaatgtgggg tgacggtttc
     9961 tggaacttgc tggcgtttgg tatgcagatg gcgcttatca tcgttaccgg tcatgcactt
    10021 gccagctctg ctccggtgaa aagtttgctg cgtactgccg cctccgccgc aaagacgccc
    10081 gtacagggcg tcatgctggt cactttcttc ggttcagtcg cttgtgtcat caactgggga
    10141 tttggtttgg ttgtcggcgc aatgtttgcc cgtgaagtcg cccggcgagt ccccggttct
    10201 gattatccgt tgctcattgc ctgcgcctac attggttttc tcacctgggg tggcggcttc
    10261 tctggatcaa tgcctctgtt ggctgcaaca ccgggcaacc cggttgagca tatcgccggg
    10321 ctgatcccgg tgggcgatac tctgttcagt ggttttaaca ttttcatcac tgtgacgttg
    10381 attgtggtga tgccatttat cacccgcatg atgatgccaa aaccgtctga cgtggtgagt
    10441 atcgatccga aactactcat ggaagaggct gattttcaaa agcagctacc gaaagatgcc
    10501 ccaccatccg agcgactgga agaaagccgc attctgacgt tgatcatcgg cgcactcggt
    10561 atcgcttacc tggcgatgta cttcagcgaa catggcttca acatcaccat caataccgtc
    10621 aacctgatgt ttatgattgc gggtctgttg ctacataaaa cgccaatggc ttatatgcgt
    10681 gctatcagcg cggcagcacg cagtactgcc ggtattctgg tgcaattccc cttctacgct
    10741 gggatccaac tgatgatgga gcattctggt ctgggtggac tcattaccga attcttcatc
    10801 aatgttgcga acaaagacac cttcccggta atgacctttt ttagttctgc actgattaac
    10861 ttcgccgttc cgtctggcgg cggtcactgg gttattcagg gacctttcgt gatacccgca
    10921 gcccaggcgc tgggcgctga tctcggtaaa tcggtaatgg cgatcgccta cggcgagcaa
    10981 tggatgaaca tggcacaacc attctgggcg ctgccagcac tggcaatcgc cggactcggt
    11041 gtccgcgaca tcatgggcta ttgcatcact gccctgctct tctccggcgt cattttcgtc
    11101 attggtttaa cgctgttctg acggcaaccc tacagacaga aggaatataa aatgaaaaat
    11161 tgtgtcatcg tcagtgcggt acgtactgct atcggtagtt ttaacggttc actcgcttcc
    11221 accagcgcca tcggcctggg ggcgacagta attaaagccg ccattgaacg tgcaaaaatc
    11281 gattcactac acattgatga agtgattatg ggtaacgtgt tgcaagccgg gttggggcaa
    11341 aatccggcac gtcaggcact gttaaaaagc gggctggcag aaacggtgtg cggattcacg
    11401 gtcaataaag tgtgtggttc gggtcttaaa agtgtggcgc ttgccgccca ggcaattcag
    11461 gcaggtcagg cgcagagcat tgtggcgggg ggtatggaaa atatgagttt agcgccctac
    11521 ttactcgatg caaaagcacg ctctggttat cgtcttggag acggacaggt ttatgacgta
    11581 atcctgcgcg atggcctgat gtgcgccacc catggttatc atatggggat taccgccgaa
    11641 aacgtggcta aagagtacgg aattacccgt gaaatgcagg atgaactggc gctacattca
    11701 cagcgtaaag cggcagccgc aattgagtcc ggtgctttta cagccgaaat cgtcccggta
    11761 aatgttgtca ctcggaagaa aaccttcgtc ttcagtcaag acgaattccc gaaagcgaat
    11821 tcaacggctg aagcgttagg tgcattgcgc ccggccttcg ataaagcagg aacagtcacc
    11881 gctgggaacg cgtctggtat taacgacggt gctgccgctc tggtgattat ggaagaatct
    11941 gcggcgctgg cagcaggcct tacccccctg gctcgcatta aaagttatgc cagcggtggc
    12001 gtgcctcccg cattgatggg tatggggcca gtacctgcca cgcaaaaagc gttacaactg
    12061 gcggggctgc aactggcaga cattgatctc atcgaagcta atgaagcatt tgctgcacag
    12121 ttccttgccg ttgggaaaac cttgggcttt gatcctgaga aagtgaatgt caacggcggg
    12181 gccatcgcgc tcgggcatcc tatcggtgcc agtggtgctc gtattctggt cacactatta
    12241 catgccatgc aggcacgcga taaaacgctg gggctggcaa cactgtgcat tggtggcggc
    12301 cagggaattg cgatggtgat tgaacggttg aattaacgaa caaaaacgcc cgatagcgaa
    12361 agttatcggg cgttttcttg aacatcgaca gcgaaggtaa ccccattaat caccagtcaa
    12421 aacttttcac cagcgtcagc tcgccagcat tacgcatcgg tacaataaat gtttcctgtt
    12481 tctcattgac cgatccttca tcggtgatca gcgtcagttg ggcggtggtt aattccgttt
    12541 cgctgcgtcc gccatagtag ttgatataca cctgatagcg tccgtgaact ggcgctggca
    12601 tggcgaaaat ctcgggtccg taccccgtcg tgacatccat atccagtgca ccactgtttt
    12661 tcagcacggt gttaccgtac caggcgtgtt cgccatcggg cgtgacaaca tggagatcga
    12721 gatcggtatt gtccgtatcc cacgagagaa ccagccgtaa acgtgcacga atcgttcctg
    12781 tacccggcgt tgagtaaaac tgcatttttt gtcggctttg cccatccggg ctgatgacct
    12841 gcacgctgtt gctgccttca gtgaaaatat aagggcgtgc aaatgaacca tcggattcaa
    12901 ttctctgcgg catactggca ccgttgacca ccagccggcc ctgctgaact ttacccgccg
    12961 cagcataatt ctttattttg ccgcggatct gcgctgagat attttgatca tctgccatgt
    13021 tgacggacga cgccgggtaa ttgatcgatt gtgaaaaact ggcatcttcg ccctctgccg
    13081 gatgccagcc agagagcggt gcgtcaatct cgacttcctg cacaccttca ctgtgagcga
    13141 caggcgaaag tgccaccagt aacaacggaa gaaaaatctt tcgcatagca ttagtccatt
    13201 aataattgtc gggtcagcgt ttcgatatag ttctcatcaa gcccggtggg ataaccatca
    13261 aaggccaggt gcagatactc atgggttaaa tccagccgat cctgaagcgt aaagaagtta
    13321 cgaataaaca gccgtttctg ctgccgatcg gtatagggga aacctgagac taaacgacag
    13381 accgcaaaca cgtctggttc gttgtacccc gtctcgcctt gtaatatacg ccgccactgc
    13441 ggcatttttt tcgccagcca ggctttcgct ttgggtaata actggcaggt tgagcgtggc
    13501 gctccccagc ggctaaggct gttgtcggga taagcaaacg cgagtatctg gtcgtaacgc
    13561 tccccctgcc ccgcctgggc cgtggcttgc cgccaggaaa gtgtcccttc ggtagcgcga
    13621 ctgccatgat aatgaaccgg atcgcctgca tagatgagat cctgcgtcca ggcggtcatc
    13681 gttcgcgccc ccgtggtcgc tggcgaagca gaaacacgct gcgtggcgct gctgtcgggg
    13741 atggtcaggc aatcgccttc ccgattggcg ttttgctgta aaaaagtgcg aatcgccacg
    13801 gtcatggctt tcgctgcctc agggggcgta cttttcgcct cgcgatccag aactctggca
    13861 acgtactctt cacgatcgag atgcgactgc aatttcagtt tgctcttttc gctcagtaag
    13921 gtcgtctcac cgtggctgac aaacgtaatg tggttaccat tggtaaacgt cacacgatag
    13981 cgcccattaa gtacgccggg ttttactgcc gtcgtactct tctccgctgt gattttcttc
    14041 agcggatagc gggcaaacag ctccacctct acacattgcc cactggcaac ctgcgtcggc
    14101 actggcaaaa cccgattaag caccgtcgca taacgggtta gtaccgtttt gctggtgcca
    14161 ctgccagtaa cccacagcgg tgtaccatcg gttaaccagc cagcaaaacc accctgacgg
    14221 atttcctgtt tatcatcggc aaaccagctc caggttttca cccgtacccg actaccgagc
    14281 atggatgcga caccgatttt cgcctcgtcc agcaccacat ccagtaagac ttcctgagct
    14341 ttgttttgcg cgggtaacgt cgctaatgaa tcaagcagcg atttaaccgt taccgaggtt
    14401 tctggcttca gcgtaggcag agacgctaac cacgcaggtg cctgacgctg ttgccagtat
    14461 tgcccccaca catctgcgcc aatatgcaac cgctgcggcg caaaatagag cccacaggaa
    14521 cgtaccagcg cggtatcgcg cgttatgctc tccccagcct gacaacaata aacctcttcc
    14581 ggggagtttc cgcgacactg gtagggctgc tccggttgat gggtatcctc cagccaggcg
    14641 taaacataca gtttccacaa actccctaac ggcgtggtga gcgagtcagg taatgctgag
    14701 actttggtca cgcctgatga cgacagctga tacagttgat cgtgctgcgc accgcgcagc
    14761 accagttgta acggcgtctc ctccgcaagc gttggcagag taaccaacgc caatagccag
    14821 acgattctgc gccagttcac ttatttcacc tgcatcccgg tccattcact ccctgccgca
    14881 acactttgct gcgcaggtgc ataggaacgc acataacgag caggaggcag aacgaattgc
    14941 cctttttgcg agaagcgcag caaatggcgg aaagtgaccg ttcccgtcag ttctttcacc
    15001 ggcaccatat aggccagttc gcccatttca ttacgcgctt tttccagcaa ttgcccctgc
    15061 tgtttcgcgg cgttgggttt attgactgaa atgccccatg ttgtgcgctc aacgtcggct
    15121 cccggcggga gcggtacttc cacctgacca tagcgcagaa ctgcatcctg ctcgctggta
    15181 agcgtaattt catcgagata cagcgcatcg ctgtcaatct cattgctggt caccggctgc
    15241 agagtaaagc tcatctcttc ttcaccgggg ataagccgat acaactggcg ttcaacggtc
    15301 accggaatgt tactttgttg agccgttttt gccggttcac gccagcggac ctgcacattt
    15361 tgcggcgata attcgtcacc aaaagagaga atgtccggca caccctgacc aacccaacgc
    15421 cagtcttcgc cccctccagt taacttatgt ttagcccatg cgcccgcagg cgcaggcaaa
    15481 acaactggag gcattgtcgc catatatttc gccagccagt tcatggccag cgcgcgctca
    15541 atagtggatt gctcagcggt caaaccactt aaaatagcgg ccgtctgggt agcgtcacca
    15601 ccgctattga gcagcacaac cgtgtgcgcc agcggctgat tgctgtttgc cgctttatcc
    15661 cacgcatatt gctgaacctg tggcatcacc gtggacttca actgcgcttt tttcagcaac
    15721 gtcatggtta agacgtttgc caccgcatct gccagtggag attccggtgt atcgaagatc
    15781 aggctgtcat tgatatcgct ggggtcttcc tcagagaaat cttcagtttt agttccgcgc
    15841 cgggcgatag cttcatccaa ccctttcaac aacgttttgc acggcagatt catctcctgt
    15901 gcccacgcca gcaccagcgc ccgatgcaat aacggcacgt tatctgcctg ctcggcataa
    15961 ctgtcgagca tatgctgcca gtattccggt tgttgcgtta cgccgatcgc ctggctggcc
    16021 tgccagtcgg cgtaccatgc ccatgccgta aggaaggcgt caccattgcc atcttcaccc
    16081 caccaggtaa agcgcgctcc gggccccgcc agttgcatca gccgcagacg gttatcctga
    16141 atcatctgac gaatgtcgtt agcggcggca ctttgatgat cggcaagcga acgccaggcg
    16201 agactgagcg ggatcagacg gctaccggtg ttgattacgc caccccacgg ttcatcgact
    16261 aacgcatcaa gattgttgcg gaaaatctcc tgcggcgttt cactactttg tagccggata
    16321 ttgctcgcct gctcgggcaa catcagcgcg ttatcgccac caccgagcat gacatttttc
    16381 tgctgttcaa cgggccagct gttatccaca aaagacagtt ttgtgctaat gctgtcctgc
    16441 acttgcccat tttgttgcag ttctgcactt aacaagccag attgctgaat attctgcgtc
    16501 agcgaaatat aattcgcccc tttgtgcagc gtcagcgtct ggcgcatctc agcgcctgca
    16561 aatttagtca ccagcgccac cggttcgtta tcctgctgac tgaagataaa cagtcctgcc
    16621 gccggtttgt cgcccacgcg atacatcgtt ggcatactcc acttcatgta gagatttttt
    16681 tccgaacgca gataagcacg cccctgcccg accagcccgt cgccgttcat cccacgcgcg
    16741 gtgatacgcc agcgggttaa cgaatcaggc atcaggaacg tgaaatacgc tttgccttgt
    16801 ttatcggttg tgagtgacgg catccatgcc gcggtatcca cctcttcacg ccgtggacgt
    16861 tcaagcattt ttactcgccg ctcgctgcgg ttagtcgcgc caggcgcaac cggctcgctg
    16921 gagagcgcct ggtcgtagct gataaacgac agactggagc tggtacgcac attgttacgc
    16981 cccagcggat agaaaaattt gccgatattc ggcgcgattt ctggttgcag cgcgtagatc
    17041 atttcatcga ccacgcctac cgttagctgc gcagaaacag gtttaccttt cagcgacgag
    17101 gttaattcga cattgaccag ttcaccaggc tggtaatggg ttttgtccgt tttcacccgg
    17161 atatcaagct gaggaacggc aactttgatc ccggcgttct gaaaactgta ctgaccatta
    17221 cgggtataca gcaccgaaaa agtgatgtta ggcgcaaagg aattgctcac tcgaacccgg
    17281 gcttcatact gggtatcgtt taaacgttgt agcgttagcc agtttgccgg atgcgaaagc
    17341 agcgactgct gttccacgcg atcgcgttcc agcgtcaata atgcttcatc aattggctcc
    17401 ggaaaggtaa tcagcatctt cgcggtttcg cctggctggt acagcgtttt atccgccacg
    17461 atatctaccg taccagtatg cgccgtgctg cccttaccgc tgacggcatg acttaaccca
    17521 gcgagaatta agccgtcttt atcgcgtaat gtcagattgt agttgccagg tttagcgaaa
    17581 ttgacggtaa aggatttgcc gcctgacggt agctctccgc tatggctcgt gcggtcttcg
    17641 agacgcaacc attcatacgt aacaggaacc tgttttgaag attccagcgc ggcataacgg
    17701 aacacaaccg actcgccgct attactgtat tgtgcggcgg tacttaaaga gtaatgcgcc
    17761 aggccgcgtt caatgaggat ctctttggtg gtggtgacgc gatacgccgc gccgtcactg
    17821 gcggagacgg ttaacaaata gcggctcggt ttatcggcgg cggggagatt taacgtcaca
    17881 tgaccactgg cgtcggacac cgtttcgctg ccttccagcg acacggggaa acgtccggca
    17941 taacgcaaat cgttaccgac cattgataat tgctgagcgc gcaaacttaa ctgcacgcgg
    18001 gcatttttta ccggctcgcc atccgggtag agcagttgca gtttgccgct gaccgcttcg
    18061 ccagttttga actctttttt gtcgagagct aaaccaatct cgaaatgtgg cttgatgtag
    18121 tttgccacgc gaaaactgct gctatagacc tgattgcggt aagcaagacg taactcataa
    18181 cctccggcta cggcattttc tggcaggcgg aaacttccct gcccgccatt gcgcgcatcc
    18241 agcgtgacat tgacggtttg caacagactg ccgttggcgt ccagcaccga aagcttcgcc
    18301 ggggcgctga cgatggggga tgaatgcaac ggatcgtgga actcgcggcc gatcacttta
    18361 acatcgacac gatcgcctgc gcgatatagc gggcgatcgg taaaaatata caagcgggtg
    18421 ttgtagattt cgctttcgta gaagaagttc tcggagacaa aaacgccgcc ttcagcatcc
    18481 ttacccagaa tgtatgaacg ttctggcgat atatgttgta actgcaaggt accgctgtca
    18541 tcggtcacac cgcgggtcat cacgccaaga ccgtcagtcc acaagatctc tgagccgggc
    18601 ttcgcttcac cctgtttttt acccgcggtc cacaccagaa gctctttgcc tgacactttg
    18661 ctaagcgcca cggtatcgga aacaaacacc accgtcgtcg cccgataccc accaaccatc
    18721 gcctcgacga ggtacagtcc cggctcttgt tggccgagag gaatataaat gttacccggc
    18781 tgcggcgaga tgaaattgct ggatacgcct tccagtttta ccccttgctg cggctcgaac
    18841 ggtttagcct gccatagtgg ataacggaac tgttccacca ggggatattt tttcagcggg
    18901 gaaaactggt tgttctgtac ataacgggaa ggtttaataa tggcattgcc gagctgtaat
    18961 tcgggtaacg cctgagtcac attctgcctt gactgagaag agaaagtacg ctgcatcacg
    19021 cggcgagatt tgccgtacca gttatcccac agccaggtta gcgtattgtt cagcccgtcg
    19081 cccaaatatt gcggttgcac cacaatgcga tgcaggtttt tctgctggcg caaaaatgcc
    19141 atcgggtcag gaatacgata caggcgaacg tccacgccgc cgtactcttc catctgatag
    19201 cgccgataat cacgccccgg cgcttccagt cgcactttcg cctcttcact gctgctaaaa
    19261 ctgctgtcag caagcaaaaa gaatgttccc cctgcgggcg gcgcatagtt actggaagga
    19321 agcgaatcat cagcattagc aagccctgtt cccacaagag acagggacaa gcacgctgct
    19381 atcgcgccag aaaatttaaa cgataaatgc caatgaaatt gggattggaa tcgttgagta
    19441 tccatcgggt gtccttccat gtcataagtt gttgcagact gactgcgcgc attccgttgt
    19501 cagttttcgt ggcgcttccg gtgtggtaga tgacgtaacg ccccatccag accattaagt
    19561 gctgggcatc gccctgatcg aaaaaaatca tatcgccagg cagcgcctgg tttatgtcct
    19621 ggccaataaa ctggctgttg tactgaatca aattaatcgc ggtcacatag ggaccggttt
    19681 tcccgttccc ctgattccag ttttgcgcca gttgacgctg ttcaggtttt aacgtcattt
    19741 caggcggcaa atactgactt gctatgccgt tactttttag ccatttgctg tcgtgaactt
    19801 tcagcgcctc gttcgctgca aatcgcacca ggcccgcaca atcctgctga taccagcgtg
    19861 gacttggccc ctggcggagc tgttcttgtg caatgcgtac aaaccaggcg cgaaacagcc
    19921 cggattgttc gacattcagc atttcgctat gggcaacaac acaacacagc cagcaaatca
    19981 gcgccagcag cccgtgcctc atagcggctg ccaggttatg ggtagccact gccaggcggc
    20041 accgggttcc atctgggcca gcttcatgac ataacgcggt tgttgagata aagcgtccag
    20101 cttcggcatt aataaagttt gtgcggcgtt ataaaaaacc ggttcgagat tcttcggcag
    20161 actggtcaac gtttcgttac gcagcagttt cgctattcct tgtggattga tatagagcgg
    20221 aacgatgcca tcagtgggta ttacatccac cattgccggg cgggttttat tcagtgtttg
    20281 cagcgcgtta ttaaccagcg tgtcatcgag ggagaaaagc agcgttttgt tttgcatcgc
    20341 cagcgacaca cggaaaaaat aatccgacat taattgatcg ggttgcgccg cctgcgcttt
    20401 cggatactgg ccgtatcggg aactcacttc gcgacgccag atttgcgctt cgccctgctg
    20461 agtctggctt accggcaaaa caccttctgg cgctttgctt tcgtgcgcac caatattttg
    20521 cgtaaacagt ttccctggca attgcgcctg ttcggcagtg ccatcaaact gaccgacaaa
    20581 cagcggggtt tgcaattttg agtcttcata ccagcacagc cccgcggcac cgtctaacgc
    20641 cccattcagc ttgtcgtttt cctggctgat gtgcgaaagc atctcttcgg caataccgtg
    20701 tgaatacggc accgccacac agaagctggc cccggcaggc atactgttcc agaccggcgt
    20761 aaaatcgaaa ctggcatcta cgctggcgga ttcatcattt aacgccacaa aactgtgcca
    20821 gccgtcgtta cccatttcga agcgtacgcc agcaaaagaa ggcattaatc gctggtagcc
    20881 aaaacccagc aacctggcgc tggccacgat gcgctggcgt actggcgttt tttcggcagc
    20941 acgctcttcc aggccaaagc tggcttgcca gcgttttttg ccgctcaaca aatcacttgc
    21001 gatcgccgtg gcttcggtat cctgctgatc atctttaaac agcatatccg tgctggaaaa
    21061 caccagcatc ttgtcctgat atgtcgcgaa catcagggcg ttattgccgt tatagcgcaa
    21121 ctgataaaca ggaatagttt cactatttat cttgatgcta ctgatttccg ttttgcttaa
    21181 ctggctgtcg ctggtagcgg caaacaacaa tggctccagc agtttgctta aaccactgcg
    21241 ctggatcagc accatataat gtgaaagatg gccttgttta tcgtgccaca gcgctgcctg
    21301 cgcgggctga tctaagagtg acgaaaagag cttatctttc agcgtgagat cgtgttcata
    21361 gacaatacga cgaatgctgc cttcaatgcc cagacgatcg gcatgattct gataatagaa
    21421 aacgaaatct tcgctcagaa catcgtggag aaacggaatg gtgaggagat ctttgggaag
    21481 ctggctcaga gagtcgctgt cgagaaagag gtccggctca ttgagatcga tttgcagatt
    21541 gttgtgcacc accagcggcg acaacgtttt ttctggccca ctgccagcat attgcaacgc
    21601 ccagacgcca gcggaaagca gtgctattgc gccaaaacct acaagaccat agaaccgcca
    21661 gcctttcgcc tttttttcac cactcattgc cacattcctt gtgtatagcc agccattttt
    21721 tacgggcaca gccaaacttt accgtgccct aatacgacaa aagcccagac tttgcagcct
    21781 ggacttttca attcaaacaa gggagatagc tcccttttgg catgaagaag taaaattatt
    21841 cttcttctgg ctcgtcgtca acgtccactt ccggagcgat ttcatcgtcc ccttccgcgg
    21901 cactgccgtc gatggtatcc agatcttcct cgtcaaccgg ttcagcaaca cgttgcagac
    21961 ccactacgtt ttcatcttcc gcagtacgga tgaggatcac gccctgggtg ttacggccca
    22021 cgatgctgat ttccgaaacg cgagtacgta ccagcgtacc ggcatcggtg atcatcatga
    22081 tctggtcgca gtcatctacc tgtaccgcgc caacaactaa accgttacgt tcggtaacct
    22141 tgatggagat aacccctttc gtcgcacgcg acttggttgg gtattccgcc actgcggtac
    22201 gtttaccgta accgttttgc gttgcggtga ggattgcgcc atcgccacga ggcacgatca
    22261 gagagacgac tttatcgcct tcacctaagc gaataccgcg aacaccggtg gtgttgcagc
    22321 ccatcgcacg gacagaagac tctttaaagc gcaccacttt accttcagcg gagaacagca
    22381 ttacttcgtc ttcgccgctg gtcaggtcaa cgccgatcag ctcatcgccg tcaaccagtt
    22441 tgatcgccac tttaccggcg gtacgcagac ggttgaactc ggtgaggaca gttttcttca
    22501 cggtaccgtt agcggtcgcc atgaagactt tcacgccttc ttcaaactcg gtcactggca
    22561 ggatcgcagt gatacgttcg tcctgctcca gcggcagcag gttgacgatc ggacgaccgc
    22621 gcgcgccacg agtggcttcc ggcaactgat aaactttcat cgaatagacg cgaccacggc
    22681 tggagaagca cagaatatgg tcgtgagtgt tcgccaccag cagtcggtcg ataaagtctt
    22741 cttctttaat acgtgcggca gatttacctt tcccgccacg acgctgcgct tcgtattcag
    22801 aaagcggctg atacttaacg tagccctggt gagagagcgt cactaccaca tcttcctgag
    22861 tgatcagatc ttccaggttg atgtctgcgc tgttggcggt gatttcagta cgacgtttgt
    22921 caccgaactg ttcacgaacc agctccagct cttcacggat cacttccatc aaacgatcgg
    22981 cgctaccaag aatacgcaac agttccgcga tctgatccag cagctctttg tattcgtcga
    23041 gcagtttttc gtgctcaagg ccggtcagtt tctgcaaacg cagatccaga atcgcctgag
    23101 cttgctgttc ggtcaggtag tacagaccat cacgcacgcc gaactctggc tccagccatt
    23161 ccggacgcgc agcatcgtcg ccagcacgtt cgagcatcgc ggcaacgttg cccagctgcc
    23221 acggattagc aaccagcgca gttttcgctt ctgcaggcgt cggcgcatga cggatcagtt
    23281 cgatgatcgg gtcgatgttc gccagcgcca cggctaatgc ttcaaggata tgagcacgat
    23341 cgcgagcttt acgcagttcg aaaatagtac gacgggtcac cacttcacgg cggtgacgaa
    23401 caaacgccgc gatgatgtct ttcaggttca tgatcttcgg ctgaccatgg tgcaatgcca
    23461 ccatgttgat accgaaagaa acctgcaact gggtctggga gtagaggttg ttgagcacaa
    23521 cttcaccgac cgcatcgcgt ttcacttcaa tcacgatgcg cataccgtct ttgtcagact
    23581 cgtcacgcag cgcgctgatg ccttccacgc gtttttcttt taccagttcc gcaatcttct
    23641 cgatcaggcg cgctttgttt acctgatacg gaatttcgtg gacgataatg gtttcacgac
    23701 cggttttggc gtcaacttcc acttctgcgc gagcgcggat atacaccttg ccgcgaccgg
    23761 tacggtaagc ttcttcaata ccgcgacgac cgttaatgat tgccgccgtc gggaagtccg
    23821 gccccgggat gtgttccatc agcccttcaa tgctgatgtc ttcatcatcg atatacgcca
    23881 gacaaccgtt gatgacttcc gtcaggttgt gcggcgggat gttggttgcc atacctacgg
    23941 cgataccgga agaaccgttc accagcaggt taggaatttt ggttggcatg acgtcgggaa
    24001 ttttttccgt gccgtcatag ttatcaacga aatcgaccgt ctctttttcg agatcggcca
    24061 tcagttcatg ggcaattttc gccagacgga tttccgtata acgcattgcc gccgcagagt
    24121 cgccgtcgat ggaaccgaag ttaccctgac cgtctaccag catgtaacgc agcgagaatg
    24181 gctgcgccat acggacgatc gtgtcataaa ccgccgagtc accatgggga tggtatttac
    24241 cgattacgtc accaacgaca cgggcagatt ttttataggc tttgttccag tcattgccta
    24301 gtacgttcat ggcgtaaagt acgcgacggt gtaccggctt caggccatct cggacatctg
    24361 gcagcgcacg gccaacaatg accgacatcg cataatccag ataggagctc ttcagctctt
    24421 cctcaatgtt gaccggtgta atttctctcg caaggtcgct catctaaccg ctatccctct
    24481 actgtatccc ggattcaaag gtcgcaaatt ataacacagc cgcgcagttt gaggtaaacc
    24541 tatacgcttt attcacatcc aatgcctgat atactcgttt gtcttgccaa ttacggagta
    24601 gaagtgccaa tgaatgccga aaaatcgccg gaaaaccata acgtagacca cgaagagatc
    24661 gctaaatttg aagccgtcgc ctcccgctgg tgggatcttg aaggtgagtt caaaccgttg
    24721 caccgcatta acccgctgcg tctgggctat attgccgagc gtgctggcgg tttatttggc
    24781 aaaaaggtgc tcgatgtcgg ttgtggcggc ggcattctgg ccgagagtat ggcgcgcgaa
    24841 ggcgcgacgg tgaccggtct ggatatgggc tttgagccat tgcaggtggc aaaactgcac
    24901 gcgctggaaa gcggcattca ggtggattat gtgcaggaaa ccgtggaaga gcacgcggca
    24961 aaacatgccg ggcagtatga tgtggtgacc tgcatggaga tgctggagca cgtccccgat
    25021 ccgcagtcag tggtcagagc ctgtgcgcaa ctggtgaaac caggcggcga tgtctttttc
    25081 tcgacactta accgcaacgg caagtcatgg ctgatggcgg tggttggtgc ggaatatatt
    25141 ttgcgcatgg tgcccaaagg cacgcatgat gtgaagaagt ttattaaacc ggcagaattg
    25201 ctgggctggg tggatcagac cagtttaaaa gagcggcata tcactgggct gcattacaac
    25261 ccgatcacta atacttttaa actcggcccc ggcgtggatg tgaactatat gctgcacacg
    25321 cagaataagt gaggttgatg tttggccgcg ccaatgcctg atgcgacgct tgccgcgtct
    25381 tatcaggcct acaaatgctc cccgtaggcc ggataaggcg tttacgccgc atccggcaac
    25441 cgtgccgact agacagtgat taccatttca ccgtcatcga caaaaaccct gccgtctggg
    25501 caaaatcatc actccctttc tgccacgcca cgctgccgcg cagggacact cgctgactga
    25561 tattgcccgt gactcccact tttatttcac cccgttgctt caccgcatcg tcactgatag
    25621 tgctgccatc ctcttcaatt tccgtcgaat ggggatcgtg ataataattc agatctaatg
    25681 ttggtatgac atgaacagcg gtacgccatt cgctgtgtaa acccagccgc gtctgaatat
    25741 catcaccctg cgattgtgac acgcgcgcac ggttagcggc ggtaaaatca tcctgctgca
    25801 cgccctgata aatcacctgc gcctgcggtt caatcaccac accacgcccc ggtaaccact
    25861 gataccccgc ctccagcgag gcgataatcc ccgacgagtg gtaatgatct gtgccatctt
    25921 cttgttcgga aacatcattg ctaaaccacg cgtattgcag ccagctatcc agccaggccc
    25981 cttgcttctg attaccgtgc tgaaaccagc ttgatgtcag cccaacggca taaccgtggt
    26041 tctggttatc ggcgcgagtt ccggtcatat tcgagcggct gtcgccctgg ttatcgctgt
    26101 agccaccaac aatcccaagc atccactcgc catccgtgcc ccagcgcccg ctaaacagat
    26161 cgccgctaag ttgcaccgta gaagtgtctt catgttgagc cagttgcccc gctgctgtgt
    26221 aatgataatc tccgccgata acacgtaaat tcagcgtctg accatcgcca cctgcgtgat
    26281 cgcgtcgctc catcataaac gcctgatttg ccgcccgcag gttattgaga taaccgccaa
    26341 ctttggcatt caacaccggc tggt
//
LOCUS       DAMZET010000051        25994 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.051, whole genome shotgun sequence.
ACCESSION   DAMZET010000051 DAMZET010000000
VERSION     DAMZET010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.051"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..170
                     /locus_tag="POB34_002167"
     CDS             <1..170
                     /locus_tag="POB34_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDI5919110.1"
                     /translation="GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDI
                     EMYVAVQPKHH"
     gene            369..1427
                     /locus_tag="POB34_002168"
     CDS             369..1427
                     /locus_tag="POB34_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDI5919111.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            1599..1928
                     /locus_tag="POB34_002169"
     CDS             1599..1928
                     /locus_tag="POB34_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HDI5919112.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(2029..2199)
                     /locus_tag="POB34_002170"
     CDS             complement(2029..2199)
                     /locus_tag="POB34_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HDI5919113.1"
                     /translation="MIGVVTKADLASMEQISLVKCWLREAGAHNVLVTNAVNNHGVTE
                     LFALLHTEDVCR"
     gene            2387..2932
                     /gene="cobU"
                     /locus_tag="POB34_002171"
     CDS             2387..2932
                     /gene="cobU"
                     /locus_tag="POB34_002171"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDI5919114.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWRTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            2929..3672
                     /gene="cobS"
                     /locus_tag="POB34_002172"
     CDS             2929..3672
                     /gene="cobS"
                     /locus_tag="POB34_002172"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDI5919115.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            3684..4763
                     /gene="cobT"
                     /locus_tag="POB34_002173"
     CDS             3684..4763
                     /gene="cobT"
                     /locus_tag="POB34_002173"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDI5919116.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            4825..5760
                     /gene="ldtA"
                     /locus_tag="POB34_002174"
     CDS             4825..5760
                     /gene="ldtA"
                     /locus_tag="POB34_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDI5919117.1"
                     /translation="MMRRVNIFCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(5816..5891)
                     /locus_tag="POB34_002175"
     tRNA            complement(5816..5891)
                     /locus_tag="POB34_002175"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5856..5858),aa:Asn,seq:gtt)
     gene            6218..7135
                     /gene="nac"
                     /locus_tag="POB34_002176"
     CDS             6218..7135
                     /gene="nac"
                     /locus_tag="POB34_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HDI5919118.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7237..8187
                     /gene="cbl"
                     /locus_tag="POB34_002177"
     CDS             7237..8187
                     /gene="cbl"
                     /locus_tag="POB34_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HDI5919119.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQISPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(8225..8300)
                     /locus_tag="POB34_002178"
     tRNA            complement(8225..8300)
                     /locus_tag="POB34_002178"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8265..8267),aa:Asn,seq:gtt)
     gene            8305..9948
                     /gene="yeeO"
                     /locus_tag="POB34_002179"
     CDS             8305..9948
                     /gene="yeeO"
                     /locus_tag="POB34_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HDI5919120.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGLMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPAVSE"
     gene            10049..10124
                     /locus_tag="POB34_002180"
     tRNA            10049..10124
                     /locus_tag="POB34_002180"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10082..10084,aa:Asn,seq:gtt)
     gene            complement(10584..11300)
                     /locus_tag="POB34_002181"
     CDS             complement(10584..11300)
                     /locus_tag="POB34_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI5919121.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(11643..13097)
                     /gene="amn"
                     /locus_tag="POB34_002182"
     CDS             complement(11643..13097)
                     /gene="amn"
                     /locus_tag="POB34_002182"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HDI5919122.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            complement(13199..14515)
                     /gene="shiA"
                     /locus_tag="POB34_002183"
     CDS             complement(13199..14515)
                     /gene="shiA"
                     /locus_tag="POB34_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HDI5919123.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            14830..15882
                     /locus_tag="POB34_002184"
     CDS             14830..15882
                     /locus_tag="POB34_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDI5919124.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEKTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            complement(16144..24127)
                     /locus_tag="POB34_002185"
                     /pseudo
     CDS             complement(16144..24127)
                     /locus_tag="POB34_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(24770..25162)
                     /locus_tag="POB34_002186"
     CDS             complement(24770..25162)
                     /locus_tag="POB34_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919125.1"
                     /translation="MSKINFVTLLFFILNIQNAIGKNEDYIGVVYWRGFDIESITGIP
                     ESQINNYGMNRCIGRNVFMRLLFHINDSYSREYDDRDVRGSITFNNDIYYVNRSGIVR
                     YKNDFYRINKKSFSHIMNQDKCKCPLID"
     gene            complement(25150..>25994)
                     /locus_tag="POB34_002187"
     CDS             complement(25150..>25994)
                     /locus_tag="POB34_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003596736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDI5919126.1"
                     /translation="SRAWLAQCGLTVERLAAQIEPVYLPERKIHLYHCDHRGLPLALI
                     STEGATEWRGEYDEWGNQMNEENPHHLFQPYRLPGQQYDDESGLCYNRHRYYDPLQGR
                     YITQDPIGLSGGLNTYSYPLNPINEIDPLGLKVIVVASDPNEAKLLQEAYAQLNTTKR
                     GQEITKPLEDSKDVYNIYTIHRDAFYCPAGTTDVSCQGKEKAVFIEPNECVKLPTAQG
                     LEVTSLAVELGHELGHAHGVHDDGGDRMNNVNLNENPIRAGLGENPRTAYVVPRVEWE
                     KCRK"
BASE COUNT         6432 a         5978 c         6370 g         7214 t
ORIGIN      
        1 tcggtgatga ttttgctaaa ccctggtatc agttctttaa tagcctcttg caggacagtg
       61 cttatgaaat gttaccaaag ccctgcttcg aggtttattt gaacaatggc gcggaagatg
      121 ggtactggga tatcgaaatg tatgttgcgg tgcagccaaa acatcactaa ttcatctcag
      181 ggcggtgtgt tgacgcgatg accactcttt tttttgaaag cgaaaagagt aagatgcgcc
      241 tttcaatttt ttcgctcctg ccgggaaatt acactgttcc cggtttgtcc gtcggataat
      301 tcagaggcgc gccttctggc cgacagatga gttatgagcg cttttaatct tcattacgga
      361 gtttctgcgt gcgtgccgat aagtcattaa gcccgtttga aatccgggta taccgccatt
      421 accgcattgt gcatggtact cgggtcgcgc tggcattcct gctcactttt ctcattatcc
      481 gcctgtttac tatcccggaa agcacttggc cgctggtcac catggtggtg attatggggc
      541 caatctcgtt ctggggtaac gttgtccctc gcgccttcga acgtattggc ggtacggtgt
      601 tgggttcgat tttaggtctt atcgctctgc agctggagtt aatttcgtta ccgctgatgt
      661 tagtctggtg cgcggcggcg atgtttcttt gcggttggct ggcgctgggc aagaaaccgt
      721 atcaaggatt attgattggg gtgacgctgg caattgttgt tggttccccg acaggtgaaa
      781 ttgatacggc gttatggcga agcggcgatg tgatcctcgg ctctttactg gcaatgttgt
      841 ttaccggtat ctggccacaa cgggcgttca tccactggcg cattcaactg gcgaaaagtc
      901 tgaccgagta taatcgggtc tatcaatctg cattctcacc gaacttactc gaacgcccac
      961 gtctggaaag ccatctacaa aaactcttga ccgatgccgt gaaaatgcgt gggctgattg
     1021 cgcccgccag caaagaaact cgtattccaa aatcgatata tgaaggtatc cagaccatta
     1081 atcgcaatct ggtttgtatg ctggagttgc aaatcaatgc gtactgggcc acgcgcccca
     1141 gccatttcgt gttattgaac gcgcaaaaac ttcgtgatac ccagcatatg atgcagcaaa
     1201 tactgctgag ccttgttcat gcgctgtacg aaggtaatcc gcagccggtt tttgccaata
     1261 cggaaaaatt gaacgatgct gtggaagagc tgcgtcagtt gctcaataac caccatgacc
     1321 tgaaggttgt ggaaacacca atctatggtt atgtgtggct gaacatggag acggcacatc
     1381 agcttgagtt gctatcgaat ctgatttgcc gggccttgcg caaataattc ctgaacttca
     1441 gaatcatctt gctgctgctt cgattcagca aggataaagg gtatgatagt gaaaagggat
     1501 aaaagcattg tcatctgcgg cagctatgag taatgttggc cctaacgaat agcggttgct
     1561 taaacgaatc cgactctcac attatcaggg gtataaaaat ggaaactacc aagccttcat
     1621 tccaggacgt actggaattt gttcgtctgt tccgtcgtaa gaacaaactg caacgtgaaa
     1681 ttcaggacgt tgagaaaaag atccgtgaca accagaagcg cgtcctgctg ctggacaacc
     1741 tgagcgatta catcaagccg gggatgagcg ttgaagcaat ccagggcatc atcgccagca
     1801 tgaaaggtga ctatgaagat cgtgttgacg attacatcat caaaaatgcc gagctctcca
     1861 aagaacgccg cgatatctcc aaaaagctga aagctatggg cgaaatgaaa aacggcgaag
     1921 cgaagtaatt cctgttttat tcaatgaggg ttgcccggca accctcattg ctcattgatt
     1981 cttttctgtg tatcaccgtc atcattctca tctgagaacc tgtcgaaatt agcgacagac
     2041 atcttctgta tgcagcaagg caaaaagttc tgtaactcca tggttattaa ccgcattggt
     2101 tactaacacg ttgtgcgctc cggcttcccg taaccaacat ttcaccaaag atatttgttc
     2161 catgctggct aaatctgctt tggtgactac tccaatgatc ggacgttaac acgaggaagt
     2221 ctggctattc cacaatgcca tcttctttcg taatggattg agaatgctgg atttttaatt
     2281 atattacgcg cagtattaat cgcgtaaatg ttttacggtc agcaacggtg ggggatccat
     2341 tgttctgttc aatgaatacc atcatacaat tgaatcagga gtcggtatga tgattttggt
     2401 gacggggggc gcacggagcg ggaagagtcg ccatgcagag gcgcttattg gggactcttc
     2461 acaggttctg tatatcgcca cctcacaaat ccttgatgat gagatggctg cacggataga
     2521 acatcatcgg caaggccgcc cggcgcactg gcgcacggtg gagcgttggc aacatcttga
     2581 tgaattaatt catgcagaca ttaacccgca tgaggctgta ttgctggaat gcgttaccac
     2641 catggtgact aatctgttgt ttgattatgg cggcgataaa gaccctgatg aatgggatta
     2701 tcaggcgatg gaacaggcga ttaatgctga gattcagtcg ttgattgctg cctgccaacg
     2761 ttgtcccgca aaggttgtat tagtgactaa cgaagtggga atggggattg tgccggagag
     2821 tcgtctggca cgacattttc gtgatattgc cgggcgggta aatcagcagc tggccgctgc
     2881 ggcaaatgaa gtatggttgg tggtttcggg tattggagta aaaatcaaat gagtaaatta
     2941 ttttgggcga tgctctcttt tattacgcgt ctgcctgtac cgcgtcgctg gtcccaggga
     3001 ctggatttcg agcattattc tcgcggtatt attacttttc ctttaattgg attattgctt
     3061 ggcgcgatta gcgggctggt cttcatggtg ctgcaggcat ggtgtggcgt accactggcg
     3121 gcactgttta gcgtacttgt gctggcgctg atgaccgggg gattccacct ggacggcctc
     3181 gccgatacct gtgatggcgt attttctgca cgtagccgcg atcgcatgtt ggaaatcatg
     3241 cgtgatagcc gtttaggcac ccacggcggt ctggcattga tttttgttgt actggcaaag
     3301 attctggtat taagcgagtt ggctctgcgt ggcgaaccga tccttgcatc gctggcggca
     3361 gcatgtgcgg tcagccgcgg gactgccgca ttattgatgt atcgtcatcg ttatgcccgg
     3421 gaagaaggac ttggcaatgt gtttattggc aaaattgatg ggcgacaaac ctgcgtcaca
     3481 ctcggcttag ccgctatttt tgctgccgta ttattgccgg gtatgcatgg tgtggctgct
     3541 atggtggtga cgatggtggc gattttcatc ctcggtcagc ttctcaaacg tacgctgggc
     3601 gggcaaaccg gcgatacgct gggagctgct atagagcttg gtgaactggt cttcctgctg
     3661 gcactgctat aaagagacgt gacatgcaaa cacttgccga tttactgaat acgatccctg
     3721 ctatcgatcc tgccgctatg tcgcgtgcac aacggcatat cgacgggtta ctcaaacctg
     3781 ttggtagcct gggaaggctg gaggcgcttg ccatacaact ggcaggaatg ccggggttga
     3841 atggcgttcc gcatgtgggc aaaaaagcgg tactggttat gtgtgccgat cacggcgtct
     3901 gggaggaagg ggtcgctatt tccccaaaag aagtgacagc cattcaggct gaaaatatga
     3961 cccgcggaac aaccggcgtg tgtgtgctgg cagcacaagc gggcgctaac gtctacgtaa
     4021 ttgatgttgg tattgatact gctgagccta tccccgggct tatcaacatg cgtgttgctc
     4081 gaggtagtgg caatattgct tcagctccgg caatgagtcg ccatcaggca gaaaagttgc
     4141 ttttggacgt catatgttat acgcgggagc tggcaaaaaa cggtgttacg ctgtttggtg
     4201 taggtgaact ggggatggca aacacgacac cggcagcggc aatagtcagc acaatcactg
     4261 gccgggctcc tgaagaagtg gttgggattg gcgcaaacct gccgacagat aaactggcta
     4321 ataaaattga tgttgtgcgc cgggcgatta cgttgaatca accaaatccg caggatggcg
     4381 ttgatgtcct ggcaaaagtg ggtggatttg atttggtcgg aatggctggc gtgatgttag
     4441 gcgctgcttc ctgcggttta cccgtgttgc tggatggatt tctttcttat gctgccgcgc
     4501 tcgcagcctg ccagatgtct cctgcaatca aaccgtatct cattccttct cacctgtcgg
     4561 cagaaaaagg ggcgcgtatt gcgctgtcgc atttggggct ggagccttat ctcaatatgg
     4621 agatgcgttt aggtgagggg agtggcgcag ctctggcgat gcccatcatc gaagctgcct
     4681 gtgcgatata taacaacatg ggcgaacttg ctgccagtaa tattgttcta ccggggaata
     4741 cgacttctga tttgaacagt taaaaacgaa aagtctgata aaagctatac ttaacggata
     4801 gctttcgcga cttaggaaag ggacatgatg cgtcgtgtaa atattttttg ctcatttgct
     4861 ctgctttttg ccagccatac tagcctggcg gtaacttatc cattacctcc agagggtagc
     4921 cgtttagtgg ggcagtcgtt tactgtaact gttcctgatc acaataccca gccgctggag
     4981 acttttgccg cacaatacgg gcaagggtta agtaacatgc tggaagcgaa cccgggcgct
     5041 gatgtttttt tgccgaagtc tggctcgcaa ctcaccattc cgcagcaact gattttgccc
     5101 gccactgttc gtaaagggat tgtggttaac gtcgctgaga tgcgtcttta ttactaccca
     5161 ccagacagta atactgtgga agtctttcct attggtatcg gccaggctgg gcgagaaacc
     5221 ccgcgtaact gggtgactac cgttgaacgt aaacaagaag ctccaacctg gacgccaacg
     5281 ccgaacactc ggcgcgaata tgcgaaacga ggggagagtt tgcccgcatt tgttcctgcg
     5341 ggccccgata atcccatggg gctgtacgcg atttatattg gcaggttgta tgccatccat
     5401 ggtaccaatg ccaattttgg tattgggctc cgggtaagtc agggctgtat tcgtctgcgc
     5461 aatgacgata tcaaatatct gtttgataat gttcctgttg ggacgcgtgt gcaaattatc
     5521 gaccagccag taaaatacac cactgaacca gatggctcga actggctgga agttcatgag
     5581 ccgttgtcgc gcaatcgtgc agaatatgag tctgaccgaa aagtgccatt gccggtaacc
     5641 ccatctttgc gggcgtttat cagcgggcaa gaagttgatg taaatcgcgc aaatgctgcg
     5701 ttgcaacgtc gatcgggaat gcctgtgcaa attagttctg gttcaagaca gatgttttaa
     5761 gagcgttggt aattagaaag caaaaagcct gctagaaagc aggctttttt gaatttggct
     5821 cctctgactg gactcgaacc agtgacatac ggattaacag tccgccgttc taccgactga
     5881 actacagagg aatcgtgtga acggggcgca tattaatggc agtgcatggt gatgtcaaag
     5941 ggtgaaatgc atttttttgt ttgtttgctg tcaatttcaa caaagtggtg agctttcaat
     6001 cttattgggt aatgaaccat cgtggtgcat accctccttt tatagggcag gggaacgcga
     6061 cagctgatta aaggagcaaa tgacgaatct atcataatct cttatttgac agttggttag
     6121 cttgtacatc aacgccaaaa taaaactggc aagcatcttg caatctggtt gtaagtaatg
     6181 gcggcacttg ggccgattct taaaaaccgg aggcaacatg aacttcagac gcctgaaata
     6241 cttcgtaaaa attgtagata ttggtagcct gacccaggct gctgaagtat tgcatatcgc
     6301 acaaccagcg ctcagccagc aggttgccac actggaaggt gagttaaatc aacaactttt
     6361 gatccgtaca aagcggggcg ttacgccaac agacgccgga aaaattctct atacccatgc
     6421 gcgggccatt ttacgtcagt gtgaacaggc ccaactggcg gtgcataacg ttggtcaggc
     6481 attatcgggg caagtctcga ttggctttgc accaggaacc gctgcgtcat ccatcaccat
     6541 gcccttatta caggcggttc gcgctgaatt tccggagatc gttatctatc ttcatgaaaa
     6601 tagtggtgca gtgcttaacg aaaaattgat aaatcaccaa ctcgatatgg cggtgattta
     6661 tgagcattcc cctgtggctg gtgtatccag tcaggctttg ctgaaagaag atctttttct
     6721 ggtaggaact caagattgcc cggggcaaag cgttgatgtg aatgctattg cgaaaatgaa
     6781 cctctttctc cccagtgatt acagtgctat tagacttcgt gttgatgagg ctttttccct
     6841 acggcgactc acggcaaaag ttattggtga aattgagtct attgccacgc ttaccgcagc
     6901 gattgccagc ggcatgggcg ttgcagtatt acccgaatcg gccgcgcgtt cgttatgtgg
     6961 cgcagtaaat gggtggatgt cacgcattac cactccttcc atgagtctct ctttgtcatt
     7021 aaatttaccc gccagagcga acttatcgcc acaggcacag gcagtgaaag agttgttaat
     7081 gtcagtgatt agttctccag tgatggaaaa aaggcagtgg caattggtga gctaagtgtt
     7141 atatcgggat ggaataagat gcgggttttt attatttgtt atgccgggca ttagacttta
     7201 acaataacgg gaaatctgaa ctgcccggag tttaccgtga atttccaaca actaaagata
     7261 atccgcgagg ctgcacgtca ggattacaac ctgacagagg ttgcgaatat gctttttacc
     7321 tcacagtcag gcgtcagccg tcatattcgg gaactggagg atgaacttgg catcgaaata
     7381 tttgttcgac gaggtaagcg actgctgggc atgactgaac cgggcaaagc attactggtc
     7441 attgcagaac gtattctgaa tgaagccagt aatgttcgtc ggcttgcaga cctgtttacc
     7501 aacgatacgt ctggcgttct cactattgca acgacgcata ctcaggcacg ttatagcttg
     7561 ccagaggtca ttaaagcttt tcgcgaactt ttcccggagg ttcggctcga gctaatccag
     7621 gggacgccac aggaaattgc gacattgttg caaaatggcg aagctgatat tggtatcgcc
     7681 agcgagcgtt tgagtaatga cccgcagctc gtcgccttcc cgtggtttcg ttggcaccat
     7741 agtttgcttg ttccacacga tcatcccttg acgcaaattt caccattgac gctggaatca
     7801 atagcgaagt ggccgttaat cacttaccga caggggatta cggggcgctc acgtattgat
     7861 gacgcatttg cccgcaaagg tttgctggca gatattgtat taagtgcgca ggattctgat
     7921 gtcattaaaa cctatgttgc tcttgggctt gggatcggat tagttgccga acaatccagc
     7981 ggcgaacaag aggaagagaa tttaatccgt ctggatacgc ggcatctttt cgatgctaat
     8041 actgtctggt tgggactgaa gcgaggacaa cttcagcgta actatgtctg gcgctttctg
     8101 gaactttgta atgcaggact gtcagttgag gatatcaagc ggcaggtgat ggaaaacagt
     8161 gaagaggaaa ttgattatca gatatagcaa aaagcccgct ataaaagcgg gcttttcaga
     8221 aatttggctc ctctgactgg actcgaacca gtgacatacg gattaacagt ccgccgttct
     8281 accgactgaa ctacagagga atcgctgttg aggcacatct taacggcgaa aaatcttttg
     8341 tcaaacccga tttttaaatt ccccaattgt ttgccgtttc tatcaacagt ttgttgcatt
     8401 tgcagacaat ttgttggcga aaatctttgc agctttgctg attctccctc attatttgaa
     8461 atgtggtttc actttctgca attaaggtcg gctttgaata tctcctctgc tttacgccag
     8521 gttgttcacg gcactcgctg gcacgctaaa cgcaagagct acaaagtgtt gttctggcgc
     8581 gagataaccc cgcttgctgt tcctatcttc atggagaatg cctgtgtcct gttgatgggt
     8641 gtcctgagca cttttctggt cagctggctg ggaaaagatg cgatggccgg cgtgggattg
     8701 gcggacagct tcaatatggt cattatggct ttttttgcag ctatcgatct tggtactact
     8761 gtcgttgtgg catttagtct cggtaagcgg gatcgacgac gagcgagggt ggcgacgcgg
     8821 cagtcattgg tgatcatgac gttgtttgcc gtactgttgg caacgcttat tcatcatttt
     8881 ggcgaacaaa ttattgattt cgtcgcgggt gatgccacga cagaagttaa agcactggcg
     8941 ttgacttatc tggagctgac ggtactcagt tatccagcag ctgccatcac tttgattggt
     9001 agcggggcac ttcgtggtgc agggaatacg aaaataccgc tactgattaa cggtagcctg
     9061 aatattctta atattattat tagcggcata ttgatttacg gccttttctc ctggccggga
     9121 ctgggatttg tcggggctgg gttgggttta accatttctc gttatattgg cgcagttgca
     9181 attttgtggg tgctggcgat tggttttaat cctgcgctaa ggatttcgtt aaagagctat
     9241 tttaaaccgc tgaattttag cattatctgg gaagtcatgg ggattggtat tccagcgagt
     9301 gtcgaatcag tgttatttac cagtggtcgg ttattaaccc aaatgttcgt tgccgggatg
     9361 gggaccagtg ttattgccgg aaattttatc gcgttttcaa ttgcggctct tatcaactta
     9421 cccggaagtg cgctcggctc tgcttctacg atcattacag gccgaaggtt gggggtaggg
     9481 cagatagcgc aagcagagat acagttgcgg catgtgttct ggctgtccac tcttggatta
     9541 acggccatcg cctggctaac ggctcccttt gccgggctta tggcatcgtt ttacacccag
     9601 gatccacagg ttaaacatgt cgttgtgatt ctgatttggc taaatgcttt atttatgcct
     9661 atttggtccg cctcatgggt gctacccgct ggatttaaag gtgctcgtga tgcccgttac
     9721 gccatgtggg tttcgatgtt gagcatgtgg ggttgccggg ttgtcgtcgg ttatgtgctg
     9781 ggcatcatgc ttggctgggg tgtggttggt gtctggatgg gaatgtttgc cgactgggct
     9841 gtgcgggccg tgctgtttta ctggcgaatg gttactggac gttggctatg gaaataccct
     9901 cgatccgagc cgcaaaagtg tgaaaaaaag ccagctgtgt cggaataaac gacaaaatgc
     9961 agattatttc agcaaacgat ttcaaattta aaaaacaggc tttgacattg tggttgggca
    10021 tcgctaatat tcgcctcgtt ctcacgattc ctctgtagtt cagtcggtag aacggcggac
    10081 tgttaatccg tatgtcactg gttcgagtcc agtcagagga gccaaatttt agttttgaga
    10141 catctctatg agtctcaaaa cagttaaaaa ataaaaatta tttttacccg gttaccttgt
    10201 aaaaaaaagt gaccgggttt tttgatttct gtgtttcgtg cgcttaatcg agtctcacgc
    10261 ttcagtgagc aaattgcccc tccccaacag acatcacgat tcgcaaggtc aaaaactctg
    10321 ttccatgaag taccgtacaa ggataaagag aaaaatgcct tatgcgggat gtcgctatcg
    10381 cgtctttatc gggaaactat gcgtaattgt cactttgcct tctgtaatca gaaaaaagaa
    10441 gaactggaat tataataaat attatttctc tgggaacgag gattttttat aagaaatatg
    10501 acgagttgaa cattatattt ttttataagg attatatgag attaaagaaa tctgccgtaa
    10561 agacagattt ctttaaaaga taattagaga tttgcgacgt tatgataaac tttttgtaca
    10621 tcgtcgtcat cttcaagggc atcaacaagc ccttcaaaga tttctaaatc ttctggggaa
    10681 agctcaactt cagattgagc aatcatttct aattctgttg ttgagaactc agtgattcca
    10741 gctgctttta gagccgcgat tcctttatga aggtcagtag gttcggtata aataacaatg
    10801 ttaccttctt cttcagtcac atcacgaaca tcaacttcag cttcaagtaa aatttcaaaa
    10861 atatggtcag ggtctgatcc tttaaataca atcacacccg tattgtcaaa catatagctg
    10921 acagaacctg ccgctccgat attgccgcct tttttattga aaattgtgcg aacgttagca
    10981 atcgtacggt taacatttga agtcaatgtc tcggcgataa tcattgagcc attaggacca
    11041 aagccttcat aacgtccctg cacgaacgtt tcatctccgc cgcctttggc tttatcaatt
    11101 gctttatcaa taacgtgctt tggaacttgt gcctgctttg cacgttcaat aacgaatttt
    11161 aaagatgtgt ttaattctgg atcgggttca ccttgtttag cagcagcata gatttctaca
    11221 ccgaattttg cataaatttt agacgttgca ccgtctttag ccgttttttt agcaacaata
    11281 ttggcccatt tacgtcccac tggaatgatc tcctcgaaaa tttatttaca ttactggtaa
    11341 atcgttaaca tgtgttaatt cggcatatta tatcataaca ggaaaaatct cgtctattag
    11401 aaataaaaat attctaaagc cataatctta tatttaattt tataaaaact tatattgttg
    11461 gcgggatttt ttataattgt gttttgaaag catacttgct tttaatatgt ggaaatcatt
    11521 aagacgataa taaaacacgt atccgcccaa ggaagcaaac agcccacgat tattgcaggc
    11581 tatttgccag gtgtcttact tactccttca catctatttc tgataagaag gttcagaact
    11641 tattatcgga acggcggctc attaaaggtt cgtaatttac gcgaatgcag tcggtcgcct
    11701 tctgcgcgca gcaaatcgat cgcccgaatg ccaatttgca gatgttcgga aatagcgcct
    11761 tcataaaaac ggttagcctg gccgggaagt ttaatctcgc catgcaacgg tttatctgaa
    11821 acacacagta gtgtcccgta tggcacgcgg aaacgatatc cttgcgcggc aatggttgca
    11881 ctttccatat caattgctac ggcccggctt aagttaaaac gaagtgcaga agctgagtaa
    11941 cgtaattccc agttcctgtc atctgtggtt accacagtac cagtacgtag ccgctgtttg
    12001 acttcctcac cgggcctgcc gctcaccagc ttggtggcgt cataaagcgc acgttgcact
    12061 tcagcaatgc tcggaatagg aatatcgggc ggcagaaccg catcaagaac gtggtcatcg
    12121 cgtaaataag cgtgtgcaag tacataatcg ccaatggcct gactttcacg taatccgcca
    12181 cagtgaccaa tcatcaacca gacatccggg cgtagcactg ccagatgatc gcagatggtt
    12241 ttagcatttg acggtcccac gccaatattc accagagtaa taccctgacc atcggcggta
    12301 attaaatgcc atgctggcat ctgatgtttt ttccatgcaa ggtcggaaat ggcttcttct
    12361 ggcgcttcgg tttcggcggt gatccagttc ccgccagcac aagaaagggc aatgtaggga
    12421 ctatcaggat cgaggatctg gctgcatccc caacgaacga attcatccac ataacgtgtg
    12481 tagttggtaa acaagacgaa cggctgaaaa tgttcaactg gcgtaccggt ataatggcgc
    12541 aaccgcgcga gggaaaaatc gacgcggcgc gcatcaaaat gcgatagcgg ggagaattca
    12601 gttggatgat aaatgccgtc tgcagtttca tcgccaattt gcgccagttc tgttgtcggg
    12661 aagtagcgag ttaacccagc gctcattgag cgatcaagtg tcaattcaga gccatcgatg
    12721 acataaggat aggggatttc atgctgcgag ggttgcaccg agatatgcgc accataatcc
    12781 tgatacagca acgtaagttg ttcattgaga tacgaacgaa agagagtagg gcgagtaatc
    12841 gtggtggtgt agctgcctgc gtgagtaaaa cgaccaaatg cgcgcgtttt ggggggattg
    12901 gttgtgctac cgtcccaggt tacggtcagt gatggataga caaaaagacc ttgtttgcgg
    12961 gcgttttcat caggtaattc gccacttgta atatagttgc caatggcgtt gcgtaatgcg
    13021 actacagatt gctcatacag cgcgtcgagt ttatccagtg cctgagctgg ggtcagaccg
    13081 gagcccttat tattcatgtg tgtctcctgt tccatacaat taccgcacat ccgatagtat
    13141 gtcacaggaa tgtgaaacaa aagtgtaagg cgaagatcgg atgatcttcg ccagactatc
    13201 aagcgcgttg attgtctttc atcaacaaag cggtcattgc ggaaatcaga catccagcca
    13261 gcaaataaat ggcgacgcta tgccagttcc cggcaaagta agtgatgagt gcagcggcaa
    13321 taaaaggtgt aaatccaccg ccaaccacac tggcgacctg ataaccgact ccagcaccac
    13381 tatagcgata actggcacca aacatttcgg taaacatcgg ttgttgcaca cacaccacca
    13441 tgtcatgcgc aatgtttgcc agcattatgg agaagaaaac tatccagaaa atagattgtg
    13501 cttcaagcgc cataaagaaa ggaaatgcgc tcaacgttcc gattaacgca cctgtgatat
    13561 aaaccctgcg gcgaccaaaa cgatcggcaa gccaggcaaa acagggaatt gtcaagcagc
    13621 ttaatccacc taccagcaaa ccaatattga ggaaaagttc gcgcggtaac cccatattct
    13681 gggttgaata attaagtgca aaggcagtaa cgatgtacat cgtcagcaat tcgcacagtc
    13741 gtagcgcaat aatcttcagg aaagcaccgg gatgtcgtaa cagcgcttcg ataaccggga
    13801 tgcgtttttt cgcggcagct tgataatgtt gctgttgttc aaattccgcg gactcctcca
    13861 tgccattgcg cacccacaat gctcccagta ccagtacgat gctaaacagg aaaggaatgc
    13921 gccagcccca gcttaaaaac tgttcgtcag tcgtcatcat actgatcaat gaaaccagtc
    13981 cggttgaaag cagtaaacct acaccgtagc caacttgtac accgctactg taaaaggctt
    14041 ttttattttt cggtgcactt tcaacggaaa gcaacgccgc gcctccccat tcgccgccga
    14101 cggcaaatcc ctgaatggca cgcagtgtca ccagcaaaat aggtgcccac cacccaatgg
    14161 tcgagaatga aggaagaata ccaatcaagg ctgtcgcgat gcccatcatc cagacggtca
    14221 gcattaacat gcgcttacgt cccagtcggt cgccaaagtg accgaaaatg acaccgccga
    14281 gcggacggaa aaggaatccg acgccaaagg tggcaaatgc ggcgagcgtt cccatcgccg
    14341 ggcttacttg cgggaaaaac tcgcgattaa acaccagtgc ggcggtgatg ccatagagta
    14401 aaaaatcata ccagtcgacg acggcaccag cgaagctgcc taacgcagct cgtcgggcgc
    14461 gacttaacga aagcgtccct tcatcgggac gagtggagat gagcgtggag tccataatga
    14521 tcctgtttgt actgtctttt tataattagt atggaaaagg taactcacat tgccgtcgat
    14581 ctataaacga accttatgag actacctcga cagagcgcat ctgcaaatgt ttttatggct
    14641 gattagcggc aacatgaaaa attcgatgga agatgaaagc gcaggagttg ctggtgttgt
    14701 ttaaggatta agcagaggac ccacaggtta gagatatgaa acataaaagt ccgcatatgc
    14761 acaaaatggc accctgatga gtctgataga ctgcattgta ttataaccag tagggaggcg
    14821 aggttaggta tgtttttagc atcattgttg agacgtattg catttagtta ctacgattat
    14881 aaagcttata atttcaatat tgaaaaaaca gactttgttg tcatccatat tcccgaccag
    14941 attggcgatg ctatggccat ctttcctgtt attcgggcgc ttgaattgca taaaattaag
    15001 catcttttaa ttgttacgtc gacaattaat ttagaagtgt ttaatgcact taaacttgaa
    15061 aagactaaat taacattagt cacaatgact atgcaggatc acgcaacatt aaaagaaata
    15121 aaagatttag caaagaacat aacacagcaa tacggtacgc cggatctttg tatagaggcg
    15181 atgcgtaaaa agaacctgaa aacgatgata tttatcagtc agttgaaagc aaaaacgaat
    15241 tttcaggttg ttggtttaac catgaagtgc tactcccctt tgtgcaagaa cgcgtcccgg
    15301 atggatcaga atctccgggc tcccgtaccc atgacatggg catttatgat gcgtgaggcg
    15361 ggttttccag cagtcaggcc aatatatgaa ttgccactaa gtgaggatgt actcgatgag
    15421 gtgcgcgagg aaatgcgatc gttaggatct tacattgcgc ttaatttaga aggtagctcg
    15481 caggaacgta cattttcatt atcgattgca gaaaatctaa tagcaaaaat tcaaagtgaa
    15541 acagatatac caatagtgat cgttcatgga ccaaaagggg aagataaagc cagggtatta
    15601 gtggattgtt ataataatgt ctaccgttta tccttatcac cctcgattaa acgttcagca
    15661 gcaatcataa aagatgctta tatcgcaata actcctgaca cctcaatatt acatatggca
    15721 agtgcctata atactcccgt tgttgcaatt tatgctgatt acaaaacgcg atggcccgca
    15781 atggccgatg tttcggagtc agtcgtcgtt gggcaaaaaa ttgacaatat aagtctggat
    15841 gaattcgcaa aggcattaaa aagtgttttg gcgagaatat gagacagtac ttatgcatag
    15901 taatgccgat ctgttatgaa cggtggtgtc atcaatactc tgaggctggt caatcatcac
    15961 agaaaaccag cctttggatc aaagcagtac tcaccggata cgatcatagt cacaccgaac
    16021 gcgattcttg cggtgcagca gttttattta ttcattttct gtgagagaag ggcaagctct
    16081 atagagggaa tgtaaaaaag agtgctccga atggagcact ctttaagaaa ttataacgga
    16141 aaatcagagg tttttataac atgtcgcata agcaaaccca agcttttcaa atacgctttg
    16201 atcacctgtt gccagggaaa ctacgtattg ttcgtttgag tttgcgggac tagatgacca
    16261 atacatattt gattgaaaac cagcttcagt cgtgtaatgc cccatatctc cccattcact
    16321 gtacagtgac ccgatgcctc gggaactact gtggtttaaa tagtttgctc tggggagcgt
    16381 gtagccattg ctgctgcaaa aattttcagc aaggctgtat atcatgaaag cctcgccggc
    16441 gttcacccac caactcttca cacggatttc gtatacatag ctagggcctc ctgattttgg
    16501 cgtcgccgta atcctttccc aattgctgcc gacattttta aatgtcactt taccggtagc
    16561 atcaacatcg acccaggagg cagagcttga aaactcataa tcagccgccg tttttcctgg
    16621 ggcaaagttg tcattattca actgataata cgccccggtg aacccctgcg aagggaatgt
    16681 agttgtcggt aggtcagtac cattgactga tactgtaccg ctcattattt tgtcttctgc
    16741 gcgagtgaat tgtattgtgg tactcagacc agcttgatga acaccattca atacagggat
    16801 cagcgttgcg ataccctctc cgccgcctgt aacagtggct ttgtaatcac cgttgatatt
    16861 tagactgtaa tcaattgcgc taatttttat atagggcacg ttagtacctg attgcacaaa
    16921 ttccatccct tcagaaactt tgatcggatt gcctgatata tcgtgcagaa caagacgtaa
    16981 ttcagcacta tcggtatagt cccctttcag tgatgaccga ttgctcttaa ggacggactg
    17041 cgaggtgtct gccgggccag ccaccagcgt tatatctact gtttgcattc caccagcggt
    17101 cgtgactgcg cttaccgtga cgctacctgt catcgctcct ttcacgcttg ttgtcgcgat
    17161 tccgttttga tcggtcaccg ctgttaatga cgttaatgtg gcagagccgc tttttaaggc
    17221 gaagtacaca gtgagacctt cgatcaggtt actactgcca tcctcaaccg ttgcctttaa
    17281 ggtacttaaa tcagtgttgg tggcggcgat agtcgatgga tcagcgataa agctagcaac
    17341 atgggcggtg cttgagttgc cagtgacttt cacggttgtc tgtgtctgta ttgttacgcc
    17401 gttatggaac gatgcagtca ccgtatatgt accgacttta ttgctggtca gaacgactgg
    17461 agcctgacct gaagagttag ttctcacttt tccgccactt aacgtcgccc cttctggcgt
    17521 tacgctaaag ttgatgacct gaccctccac tggagtgcca tttgcagagg ttagcgttgc
    17581 cgtcagagta gcacctgtag gggcatagac accgataagc ggactggacg ccgtgagtgt
    17641 cagtttttca tcaatagcct ccagttgttt ctcaagtgag gctccattcg ccagggatgc
    17701 tttcaccata tacgaaccgt ttctttcggg cgtcatggtg acctcggcta caccctgcgt
    17761 attagtagaa accgtattct ggctaatgat catttgcgag gatggctctg cactgaatgt
    17821 cacgggttga tgcgcaaccg ggttgccaaa ctggtcgtta acgtgagctt taactgctac
    17881 gtcttgtgcg accattacct gaccttcagg tatctccaga ctggtaatcg tcgcagaatt
    17941 aacatctgca ctggcattca gtaatcgcga gatgacttca gtaggtttat ctgccagaga
    18001 ggctgaaact gttttcagtc cgacctcggt gcttgtcaca agaatttcag cgaaaccccg
    18061 atcatcagtt tcaacgctgg tgctgcttag cgtgacggag gttccgcgga aattaacttt
    18121 tatgccttct accgggttgc cgttagcgtc agtgacgctt gcagtcatgg tcgcgccctc
    18181 agtcgtgctg acgacgaatg agtatacaga ggttaaggag gctagttttg cggtaccagc
    18241 atcggcaatc aacgtcacct gtttcgtatc actgacacca taattgttca cgctaactgt
    18301 cacggggtag gtgccggatt ttgtaccgct cagtgtaact tcagccttgc cgttgatgtc
    18361 agtaatggcg gaaccgcctt gtccgagagt aaagcttgcg ctcacatctg ccggtagcgt
    18421 gaatgtcacc gcctcattgg ctaacgggtt gccgtttcca tcagtcactg ttgcctgcag
    18481 cctggtcatc ccgacgttat tagcgataaa attgtcctcg gtaacgttaa gattaacctg
    18541 cgcagtgagc gtatccgcaa taaagttcac caccaactgc tcactcttac cgccagtcat
    18601 cgatgctgta acagtatgag cgcctgcttt tgtacctttc agcgtgactt tcgctttgcc
    18661 ttcagcatca gtaatcgctt taccgccatc gctcagcgtg aagttcgcct tcacatcttc
    18721 cggcagagta aatgttactt cagtgttggc tatcgcattg ccctctgtat cagcgactgt
    18781 tgctgttagt gtcgtgagat cgttattgtc ggcaatcaac ggatccttcg aggctgccag
    18841 cgtgatttcg gcattcgcga cgttcggcac ataggtcact gtttgttgca tcgaatcgcc
    18901 attttcgagg gttgccgtca cttgataaac cccggctttt gtagcggtaa agcttgcgta
    18961 aaaattgccg tcgtggttgg tagtatatat agcgttatta ctgggggtca cgccttcact
    19021 tggtgatact ctgagggtta cctcctgctg gggtacaccg ttgccgtaat tatccttcac
    19081 ctcaatatac agattggtag tgtcgcctga ggagactgta tcaaaaagtg cctgtagcaa
    19141 ggtgagatgt gccgtagacg catcagcgtt gacattaatt gatgtgctaa gcgtggagct
    19201 accattttcc agactggcct caacggtatc cggtctcgct cctgattcta tcgaggagcg
    19261 ggtattggta taagtgacgg tagccttacc ctgttcgttc gttacggctt ggccgccatt
    19321 cgtcatttcg gcagagtttg ttctgctggt gaagttcaca gtcacacctt tcaccggaaa
    19381 gacattatta tcaacgactg tggcggtgat gacgctgccg gagctgttct gcggggtacc
    19441 ggcgattatg ctgtctggga caggcgtcaa ctcgataatt tttgccgctg ctgtgtcgcc
    19501 aataaaatgc acagttttgt tgtcgctggc accattatta gccagtgatg cagtgaccgt
    19561 ctgctcacca aaggcaacgc ctgcgagagt ggcctgcgcg atgccagact cattggtatt
    19621 acttactccc ggggtcaggg tgagtcctga agaggctgag ctgaatgtta ccggaagatt
    19681 attcacctca ttgtcgaact gatctttaac cgttgcagtt agcgttgcgt tatcgacgcc
    19741 attacctgtg atctcatctt ttgacatctg caggacaacc tgagccgagg ttttgtccgc
    19801 cacaaacgtt accggctgcg aatcactggt attgttatta cccagcgttg cggtaaccgt
    19861 atgcgtaccc gcttttttac ctttcagcgt gacatgcgct tccccattgg cttgggtgat
    19921 ggcaatgccg ttattttcga gggtaaagtt tgccgcaacg tcctgtggca tggtgaaggt
    19981 caccgttatt cccgccaccg gatgattcga cggatctttc accgtcgccg tcagctgcac
    20041 gctgtcgctg ttattcgtca ccacctgttg agcagggatg ttcagggtga cctgcgcgtt
    20101 gctggcatcg ggcgcaatat tgactgtcgt cgtatagcca ttgccgttaa gcagtgtggc
    20161 ttcgactgta tgaacaccca aaaccgtgcc cttaagggta acttcagcaa caccactgtc
    20221 gttagtattg ctcgtgctct gactaagttg agtatctgcc gggttggtac tgaaggtgac
    20281 cggtaggtct tttactgcgt tatcaaaagg atctttcact gttgccgtca gagtcgtctc
    20341 atccacgcca ttcccaatga tttccgcttt cgatgtttgc agaacgacaa ctgcgctgtc
    20401 cttatctgcc acgaaggtga ccggttgcgc atcgctggca ttattgttac ccagcgttgc
    20461 ggtaaccgta tgcgtgcccg cttttttacc tttgagggtg acatgcgctt cgccattggc
    20521 ctgagtgatg gcaataccgt tattttcaag ggtaaagttt gccgccacgt cctgaggcat
    20581 ggtgaagttc accgttattc ccgccaccgg atgattcgac ggatctttca ccgtcgccgt
    20641 cagctgcacg ctgtcgctgt tattcgtcac cacctgttga gccgggatat tcagcgtgac
    20701 ctgcgcgttg ctggtatcgg gcacaatatt gacgattttc gtatcgcttt taccgttaag
    20761 cagtatggct tcggctgtat gaacacccaa aacagtgccc ttaagggtaa cttcagcaat
    20821 accgttctca ttagtattac gcgcgttcag actcagttgc gtgtctgcgg gggaggtgcg
    20881 gaagactact gacagatttt taaccacgtt atcaaaagga tctttcactg ttgctgtcag
    20941 agtcgtctca tccacaccat tcccaatgat ttccgctttc gatgtttgca gaacgacaac
    21001 cgctctgtct ttatccgcca caaacgtcat tggctgtgta tcgctgacat tgctgttagc
    21061 cagacgagcc gtgatcatat gcgtgcccgc taatgtgccg gtcagggagg cgattgctat
    21121 cccgttgcta tccgtcatgc cttttccgct gttgctaatc gagaaccgac ttgcgacgtc
    21181 gtttggcaca gagaaggtga tttctttatc ttttagtggg ttgccatttt tatcctgcaa
    21241 ggttgcagtc atatgtagcg gagctgtgtt ggtgacggtg atatcacctg aaggcacact
    21301 gagggtcagg gcagcagtac tttgatcacc gataaaaatc acctgttgat tagcctgaga
    21361 accagagctc acagaggccg taaccgtata atcaccattt ttcaaactgg tcagcgtagc
    21421 tgtggcgatc ccgtcgtggc tattcacttc ggtttggctc agtttcgctg ctgctgaatt
    21481 aacattgaag gtgaccttga cgtcattgag caggttgcct tttgcatccc ggactgtcgc
    21541 ggtcattgtg gcgctgtcat tgccgtcagc gaccacttca tttttcgact tctgcagatc
    21601 aacctgcgcg gtacttgagt cgccgacaaa actgacgatt aacgtttgtt tcacgccatt
    21661 ttcaagggtg acttcaaccg tgttgtcttc ctgcttacta cttttcagat caaaagtcgc
    21721 cagaccatta acatccgttt ttgcggtgtt ttgattgttg aaggaagttg ccgatccgct
    21781 taataccgca aacgtgacgg tatgatcatt gattgggttg cttccttcat cagcgacatt
    21841 gaccgagacg gtgtttgctg cattctcatt ggcgagcaca ccattattgc tggcagataa
    21901 tgtcgcaatt ttcgctgact gcgggttggc gtcgatgata aatccagcgg tatgtaaatc
    21961 ttcattccag ttttgcatta atagcttcgc agtaagccca ctgcctttgg tataggcggt
    22021 ataggttgcc ttatagacgc catctgcggt ttctttccag tctgtagtga caccaggttt
    22081 cacgttgtcg atgctgactg cggtattcag ttgctgtttt tgctccttaa caggtttgtc
    22141 attttcatct ctcagttcta ccgttacctc gataggattc ccggagagat aacggtcttt
    22201 atcaatttta attgacgagt gagttcggga tgacgaaata gaaatgatat tcaccacggc
    22261 gggggcttta gccgcatcca caccattcag ctgtggcatc agcgtcagcg tgccagacat
    22321 cgctcctgtg gtcaggatct gggtatagct tccgtcacca ttatctttcc attcagaaag
    22381 ggtgatgtcc tgaacacctt cgtgacgcgt cgagagcacc agcccgacaa caggattacc
    22441 tgctgcatca tgcgcaataa aagtcagtgt ggcggttgaa tgggaatccg cgttcaattg
    22501 tttgggtact taacgatacc gaggaatctt tctggcttag cgtaggtgcc tgaacgacca
    22561 ccatgctctg ttcacgattc gacaaattgc ctttgacatc ttcggcggtg acttcaatcg
    22621 gccaggtgtt atcggtttct ggcgtactgg tgaaccggta agccggcagg gtaaccagaa
    22681 tatctttacc cgttgtgacc actttgccac cggcagcttc cagagcggtg gcttcgacgt
    22741 tatagccttt cagggcatat ttggtttgta gcgacgaaac caatgatttc acttctcctg
    22801 acttccctgt cacggggtct gtcagggtca ggcgaaccag ttcttttttg cggtattcca
    22861 gaacgatgtt gttgttgcga tccaccagat cataacggct gcctgcaagg ctacgccgtg
    22921 cagcgacttc gttcgggtca agctgtttct gcattgcgct gccaggttgc caggtaaaat
    22981 cgacggcaaa acgggtgtca ttttcgccct gtttaccctg gcgttgctcc gcgctgaagg
    23041 tcatcagcgg gaagggggta tagttaagtc cagcggttat ggcatgagga ttactttgcc
    23101 gatcgtcttc atcgaacagg accacttcat cgccataata ctgttcatag accagtttac
    23161 cgccaaggtg cggccaggcg ggtagccagc cttctgcgcg tacatcccag ccattggccg
    23221 ggcgggcttc ataatcgttg tccagctcag gtgcgctgcg ccagttggtc agtcgcaaat
    23281 agccgttact gcttaatttt agatagtcgc gccagtactc cgcgccaatg ccggcgcggg
    23341 agtggtaacg gctaagatcg tggtcgaaaa agaagttgat gcccgacatc catgtgggag
    23401 tgaaatgacg ccaacctaag ccgttgttaa tctgcgtacg ctcgtcagta cgatggagag
    23461 tatgctgact gaaaaagaga ttatcaggcg tttcatacca cggatggaga aaatcgaact
    23521 gggagttctt caggctaaaa tcttcattca cgcccagcgt gattcttgcg gtaccgaagc
    23581 ggcttaacca gtctgtcatt gcgcctgaag cctgagaaga ggcccatcca cgcgccatat
    23641 ttgccgcttg ctcgctgttc atatcttcgg cgagcagaga cccgatttgc tgtgaagtac
    23701 tggctatctg ttgctcaagg ttgccactgc tattacccgg tggcggggtt aaattatttt
    23761 cactaacttg tgccgggaca tccagttcat caccctggcg gacattatca aaacctcgag
    23821 caaacgtacg aaactggttg agtttgcgta actcagccac cgaaataccg aaacgttcgg
    23881 caacgctttg ggccgattcc agcgctccaa gggtgtaggg caccgtattg gcatttgcaa
    23941 tggcaatttg tgcaggaact ggttgttggg ttgcggcatt taccacacct tgtgctgccg
    24001 cagccatagg gaacgcaagt tgagttatca gacagatacc cgctgttaag cggcgtagtt
    24061 taattcccat cccgcataag atttgtcggt catttatttc ttctccactt ctcttcttcg
    24121 tagccatgct taccttccct gaacgagcta acataaatgc gccctttcta gcacagcgaa
    24181 aaaatgtaaa tcctgatagt aatatgattg aaaattaata acttttcgtt aggcagtttt
    24241 gggtgtgagt tgcaagaggg gagactactg aataactcaa gttttataat cgaggggaaa
    24301 atggtgatgg cgttcatagc aaaacgccct caaccataaa ggtcgagggt gcttaagatg
    24361 ttaaaaaccc gctatccgtt aaaaaacaat gttcaactaa ggttagtgac attgcgctaa
    24421 gaagcgaatt tttagaattt ggctcctctg actgggatcg ccgtagccaa taaatagctg
    24481 attggtaagc taaaaaaaat cgagtttgtc aagaccacca ggctgaccac cttttgttcg
    24541 agcgacacgg agacttcagt tgagtacaga atagttcctt gccaccgggt tcgatctggc
    24601 tgagctattg ggactgactc agcacttcac ggcctgacac agttgaccgc aagtccctgc
    24661 acaccggaaa aagtcagcgc aatctacacc tggtcgtctc ccccggagtc ttacgttgta
    24721 ccgatattag gcactattta gcttgccgcc ttcccgcaac tcgaactatt taatcaatta
    24781 acggacattt gcacttatct tgattcatta tatgactaaa tgatttttta tttattctat
    24841 aaaaatcatt tttatatcta actataccag atcgatttac ataataaata tcattattaa
    24901 aagtaatact acctctgaca tctctatcgt catattctcg actatatgaa tcattaatat
    24961 gaaataataa cctcataaaa acatttctac caatacatcg gttcattcca taattattta
    25021 tctgactttc aggaatccct gttattgact caatatcaaa ccctcgccaa taaaccaccc
    25081 ctatataatc ttcattttta ccaattgcat tttgaatatt taaaataaaa aacaatagtg
    25141 tcacaaaatt tattttcgac atttttccca ctccactcgt ggtactacat atgcagtccg
    25201 tgggttttca cctaaaccag cgcggatagg attttcattt aagtttacgt tattcattct
    25261 atccccacca tcatcatgga ctccatgagc atgacctaat tcatgtccta attcaactgc
    25321 cagtgatgta acctccaggc cttgtgccgt aggtaatttt acacactcat tgggctcaat
    25381 aaacaccgct ttttcttttc cttgacagga gacatctgtt gttccggcag ggcaataaaa
    25441 agcatcgcga tgtattgtgt atatattata tacatctttc gaatcttcta acggttttgt
    25501 tatttcttgt cctctctttg ttgtatttaa ttgtgcatat gcctcctgta ataattttgc
    25561 ctcattagga tcactagcta caacgatcac ttttaatcct aaaggatcta tttcatttat
    25621 tggatttaat ggatacgaat atgtattcag cccaccactc agcccaatcg gatcctgggt
    25681 gatataccgt ccctgcaacg gatcatagta ccggtgacgg ttatagcaca gccccgattc
    25741 atcatcgtac tgctgccccg gcaggcggta cggctggaac agatgatgcg ggttctcctc
    25801 attcatctgg ttcccccact catcatactc cccgcgccac tccgttgcac cttcagtact
    25861 gataagcgcc agcggcagtc cccggtggtc gcagtgataa agatgtattt ttcgctcagg
    25921 caaataaacc ggctctatct gtgccgccag ccgctccacc gtcagcccgc actgcgcaag
    25981 ccacgcccgg cttt
//
LOCUS       DAMZET010000052        25955 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.052, whole genome shotgun sequence.
ACCESSION   DAMZET010000052 DAMZET010000000
VERSION     DAMZET010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.052"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..821
                     /locus_tag="POB34_002188"
     CDS             <1..821
                     /locus_tag="POB34_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDI5919127.1"
                     /translation="ILYSPEEDINEREQIMYQAVHYELVASALAVQTGKSINPEFNIG
                     CMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARGYYPQHMLNYFARKGFNLDIT
                     PEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVEPRDLVSNPYIDTSEWGWQID
                     PAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTVNDHYRIDYFASHIREMKKAV
                     VEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKDNEGKGTLERIRKASFY"
     gene            complement(917..1135)
                     /gene="marB"
                     /locus_tag="POB34_002189"
     CDS             complement(917..1135)
                     /gene="marB"
                     /locus_tag="POB34_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HDI5919128.1"
                     /translation="MKLLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(1167..1550)
                     /gene="marA"
                     /locus_tag="POB34_002190"
     CDS             complement(1167..1550)
                     /gene="marA"
                     /locus_tag="POB34_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HDI5919129.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(1570..2004)
                     /gene="marR"
                     /locus_tag="POB34_002191"
     CDS             complement(1570..2004)
                     /gene="marR"
                     /locus_tag="POB34_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HDI5919130.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            2216..2881
                     /gene="marC"
                     /locus_tag="POB34_002192"
     CDS             2216..2881
                     /gene="marC"
                     /locus_tag="POB34_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HDI5919131.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(2906..4096)
                     /gene="ydeA"
                     /locus_tag="POB34_002193"
     CDS             complement(2906..4096)
                     /gene="ydeA"
                     /locus_tag="POB34_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HDI5919132.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGSGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(4246..5360)
                     /gene="yneK"
                     /locus_tag="POB34_002194"
                     /pseudo
     CDS             complement(4246..5360)
                     /gene="yneK"
                     /locus_tag="POB34_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            complement(5438..6319)
                     /locus_tag="POB34_002195"
     CDS             complement(5438..6319)
                     /locus_tag="POB34_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI5919133.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            6420..7808
                     /gene="sad"
                     /locus_tag="POB34_002196"
     CDS             6420..7808
                     /gene="sad"
                     /locus_tag="POB34_002196"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HDI5919134.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            7872..8798
                     /gene="glsB"
                     /locus_tag="POB34_002197"
     CDS             7872..8798
                     /gene="glsB"
                     /locus_tag="POB34_002197"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HDI5919135.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            8798..9157
                     /locus_tag="POB34_002198"
     CDS             8798..9157
                     /locus_tag="POB34_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HDI5919136.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            9296..10713
                     /locus_tag="POB34_002199"
                     /pseudo
     CDS             9296..10713
                     /locus_tag="POB34_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            10940..12391
                     /gene="uxaB"
                     /locus_tag="POB34_002200"
     CDS             10940..12391
                     /gene="uxaB"
                     /locus_tag="POB34_002200"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDI5919137.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            12598..13512
                     /locus_tag="POB34_002201"
     CDS             12598..13512
                     /locus_tag="POB34_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HDI5919138.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(13516..14274)
                     /gene="tam"
                     /locus_tag="POB34_002202"
     CDS             complement(13516..14274)
                     /gene="tam"
                     /locus_tag="POB34_002202"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HDI5919139.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLQQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(14331..14621)
                     /gene="lsrG"
                     /locus_tag="POB34_002203"
     CDS             complement(14331..14621)
                     /gene="lsrG"
                     /locus_tag="POB34_002203"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HDI5919140.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            complement(14645..15520)
                     /gene="lsrF"
                     /locus_tag="POB34_002204"
     CDS             complement(14645..15520)
                     /gene="lsrF"
                     /locus_tag="POB34_002204"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HDI5919141.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            complement(15547..16569)
                     /gene="lsrB"
                     /locus_tag="POB34_002205"
     CDS             complement(15547..16569)
                     /gene="lsrB"
                     /locus_tag="POB34_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HDI5919142.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     VPDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            complement(16581..17573)
                     /gene="lsrD"
                     /locus_tag="POB34_002206"
     CDS             complement(16581..17573)
                     /gene="lsrD"
                     /locus_tag="POB34_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HDI5919143.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            complement(17573..18601)
                     /gene="lsrC"
                     /locus_tag="POB34_002207"
     CDS             complement(17573..18601)
                     /gene="lsrC"
                     /locus_tag="POB34_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HDI5919144.1"
                     /translation="MLKFIQNNREITALLAVVLLFALPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            complement(18595..20130)
                     /gene="lsrA"
                     /locus_tag="POB34_002208"
     CDS             complement(18595..20130)
                     /gene="lsrA"
                     /locus_tag="POB34_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HDI5919145.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMTDRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            20379..21332
                     /gene="lsrR"
                     /locus_tag="POB34_002209"
     CDS             20379..21332
                     /gene="lsrR"
                     /locus_tag="POB34_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HDI5919146.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSSLKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            21411..23003
                     /gene="lsrK"
                     /locus_tag="POB34_002210"
     CDS             21411..23003
                     /gene="lsrK"
                     /locus_tag="POB34_002210"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HDI5919147.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDIYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            23534..>25955
                     /locus_tag="POB34_002211"
     CDS             23534..>25955
                     /locus_tag="POB34_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919148.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     AGSNGQVVVEKGGEWLIKNNDSSIEFQIGNQGMGEATIREGGLVTAENTIIGGNATGV
                     GTLNVQDQDSVITVRRLYNGYFGNGAVNISNNGLINNKEYSLVGVEDGSHGVVNVTDK
                     GHWSFLGTGEAFRYIYIGDAGDGELNVSREGKVDSGIITAGMKETGTGNITVKDKNSV
                     ITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGENGVGNVSITTGGMWEVNKNVYT
                     TIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLMDGTSSFDTVGINVGNFGSG
                     IVNVSNGATLNSTGYGFIGGNASGKGIVNISTDSLWNLKTSSTNAQLLQVGVLGTGEL
                     NITTGGIVKARDTQIALNDKSKGDVRVDGQNSLLETFNMYVGTSGTGTLTLTNSGTLN
                     VEGGEVYLGVFEPAVGTLNIGAAHGEAAADAGYITNATKVEFGSGEGVFVFNHTNNSD
                     AGYQVDMLITGDDKDGKVIHDAGHTVFNAGNTYSGKTLVNDGLLTIASHTADGVTGMG
                     SSEVTIASPGTLDILASTNSAGDYTLTNALKGDGLMRVQLSSSDKMFGFTHATGTEFA
                     GVAQLKDST"
BASE COUNT         6564 a         6171 c         6594 g         6626 t
ORIGIN      
        1 gtatcttgta ttcgccagag gaagatatca atgagcgcga acaaataatg taccaggcgg
       61 tacattacga gttagttgcc agtgccctgg cggtacagac tggaaaatcg atcaatcctg
      121 aatttaacat tggctgtatg atcgccatgt gtcccatcta tcctctgacg tgtgcaccca
      181 acgatatgat gatggccacg aaagcaatgc atcgtcgtta ctggtttact gatgttcatg
      241 ctcgtggata ttatccgcaa catatgctga attactttgc caggaaagga ttcaacctcg
      301 atatcacacc agaagataac gcgattcttg ccagtggttg tgtcgacttt atcggcttta
      361 gctactacat gtcttttacg acacaatttt cgcccgataa cccgcaactg gattatgttg
      421 aaccacgaga tttggtcagc aacccttata tcgatacatc cgaatgggga tggcaaattg
      481 atccggcagg gctacgttat tcactcaact ggttctggga tcatttccag ttgccgctgt
      541 ttattgtcga aaatggattt ggtgcggttg accagagaca agctgacggc acggtgaacg
      601 atcactatcg cattgattac tttgcttccc atattcggga aatgaaaaaa gccgtagttg
      661 aagatggtgt tgacttaatt ggctacaccc cgtggggctg cattgacctg gtttctgccg
      721 gaacagggga aatgaaaaag cgctacggaa tgatttatgt cgataaagac aacgaaggga
      781 agggaacgct ggaacggata cgtaaagcgt cgttttacta gtatcgggat ctcatcgcca
      841 acaatggcga aaatatttga gacttgagga agatattacg gagatgtcag gctctgtgat
      901 cagagcctga caataactac atagcgtgtt gattataata gggcacgccg agcgcatccg
      961 acttatcact gccagtaccc atgtgattta aatcaaacgg tggttgttcc tgcgatgggg
     1021 gaacaaccac gacattggca caagaagtaa caactggctg cgtggtttgt tccgcaacgc
     1081 cctgcgcgga aaagagaata agcgcagctg ctattgcgga tgaaagtagt ttcatgattg
     1141 cctcagtgac gttgtcacgt tatcaactag ctgttgtaat gatttaatgg atgtaaaaag
     1201 cgcgattcgc cctgcatatt ggtcatccgg tatttatgcg gcggaacatc aaagtaattt
     1261 ttgaaggttc gggtcagagt ttgttgcgac tcgaagccat atcgttctgc cagatagagt
     1321 atcggctcgt tactttcctt cagcttttgc gcgatttccg tcatcttacg gctgcggatg
     1381 tattggccta atgaatgacc ggtttctttt ttaaacatcc gttgcaggtg ccatttagag
     1441 taacctgaac gctctgacac tttctccagt gacagtggcg attccaggtt gtcctcgatc
     1501 cagtccaaaa tgctatgaat ggtaatagcg tcagtattgc gtctggacat cgtcatacct
     1561 cttttttgtt tacggcagga ctttcttaag caaatgctca agtgttgcca cttcgtccgc
     1621 cgtcaggttt tttgttaatt cttggtgcag gtcctggcca actaattgat ggcattgttc
     1681 acatattgcc gcgccgctgg tggtaagttt taccagtacg ccgcgcttat cattcgggtt
     1741 cggcaacctt tcaacccagc ctttacagac caggcgatcc agcatacggg tgagtgctcc
     1801 caggtcgacc gacaacactt ttttcagttc aaccggagta atacacgccg cgcagcggat
     1861 agagcagagc accttaaact gtgccgcggt aatatccagc ggagacagat actcgttaag
     1921 cagacgatct ttcttctgat taaccatatg gattaagcga cccaatggaa taatttcatt
     1981 gaacagatcg ctggtacttt tcacattagt tgccctggca agtaattagt tgcaggggat
     2041 aatattgccc aggcaagtat aagtcaaatg aatgaattga ccgatgccac gttttgctaa
     2101 atcggttcaa gttttggtca atgcgatgca aggttagcta aagagtgaca ctttatcctt
     2161 gtttacccct tatacttttc gctgataacc cagatacaca ggataacaac caccaatgtt
     2221 agatttgttt aaagcaattg gcctggggct ggtggtgttg ctgccgttag ctaacccatt
     2281 aacaactgtc gcgctatttc ttggcctggc aggcaacatg aacagtgccg aacgtaatcg
     2341 tcagtcgttg atggcctcgg tgtacgtatt tgccatcatg atggtggcgt attacgccgg
     2401 gcaactggtg atggatacat ttggcatttc gattcccggt ctgcgaattg caggcggctt
     2461 aatcgtcgcg tttatcggtt ttcggatgct ctttccgcaa cagaaagcga ttgattcacc
     2521 ggaggcgaaa agcaagtcgg aagagctgga agatgaaccc agtgccaata ttgcctttgt
     2581 gccgctagca atgccaagta ccgctggtcc gggaaccata gcgatgatta ttagctcggc
     2641 gtccacagtg cgtcagagtt caaccttcgc tgattgggta ctgatggttg cgccgccgct
     2701 gatctttttc ctggtggcgg ttattttgtg gggaagccta cgcagctccg gcgcaattat
     2761 gcggctggtg ggcaagggtg gcattgaagc gatctcccgt ctgatgggat tcctgctggt
     2821 atgtatgggt gtgcagttta ttattaacgg catcctggaa atcattaaaa cgtaccatta
     2881 aaaaaggccc gaatgggcct ttcaactatt gcgtctgttc ttcgagtgtc actggccagc
     2941 ggcgaaatat aatgattgac caaattaacg cggcaaaagc aggcaccgcg cccacataac
     3001 caatcatcga cattgaccag tgcaaactca cctgattacc taccaacgca cccgccccga
     3061 ttccaatatt aaatatgccg gagaatagcg ccatcgcgac gtcggtagca tctggtgcca
     3121 gcgccagcac tttaacctgc ataccaagcc cgatgatcat catcgcgatc ccccagaaaa
     3181 tactcagcac cccgaggtgt atttcactgt tcgccgcagg taacagcaat gccaggcaca
     3241 ccagcaacag cgcaatcgcc gtactcacca acgcagacgc atactgatta cccagtttac
     3301 cgaaaatcac gctgccaata atgcccgaac caccgagtaa taacagtaat gccgtggcaa
     3361 agttggcgct gaatcccgca atgttttgta caaaaggctc gatatagctg tatgccgtgt
     3421 aatgggcggt gacaaccacc acagttaaca aataaatgct catcaatgcc gggcggcgga
     3481 acaatagcgg gaggcttttc agtgaaccgg aatgctcact gggcagtaag ggaagtaact
     3541 taatcaggca caaaagggtg ataagcgccc caataccaat cgcgaagaag gtcattcgcc
     3601 aaccgaaata ctggcccaca atgcgcccga gaggtaaacc taagaccatc gccagtgctg
     3661 taccggtggc aattaaactc aatgcctgtg ctcgcttccc ggccggagcc atacggatcg
     3721 ccagagatgc cgtaatcgac cagaaaatcg catgtgcaaa agccacacca atgcgactga
     3781 tcaccagaac ggtaaagctc cacgacaaaa acgacagtac gtggctggca ataaacacca
     3841 caaacaggca gatcagtaat ttgcgccgtt caacctgact ggtcattaac ataaaaggca
     3901 atgacattag cgctactacc catgcgtaaa tggtcaacat gatgccgacc tgagcggttt
     3961 gcatgtgaaa actttgcgca atgtcagaga gcaggccaac agggacaaat tcggtggtgt
     4021 tgaagatgaa ggcggcgact gccagcgtaa cgacccgtag ccacgccact ttgcgggaaa
     4081 cagtgtttgt tgtcataaca gattatcggg tgagttgcga aaaagtgatg aaatgatctt
     4141 aaaacggtta atgatgtgaa tacaatgcat ttgtgatctg gatcgcgttt tccgtttctc
     4201 tgacgctgat tctgttccgc gtcagagagt cggtaacatc acggtttaat gacgttcagg
     4261 gaggtctggc gttttggcgg ttgcgggtat aggtggtctg acaggggagc gaggcctgtt
     4321 tttcaccagg tcttcgtgag taacttttgg cggcgtttca ttttttacaa acagtttgag
     4381 ggtcctgcgc gccatatgtt tgataatttt atcatcactt aatgatgctg gcatcaacac
     4441 gtcattcaga tcactttcat tgggattcat aaattggtga tagtgcaata acatattggc
     4501 gaaaaataaa aaaaagtcct gaaaatgagg agattcttgt tctgctaaaa gggataaaaa
     4561 gcagatgcag acttcgggtt gtacatgttt gttagaacct aatgaatcca tcaggctttg
     4621 caataaatcc tcttttaatt cgccgtctgt tatgtcgaga atggcggtga tcaagttagg
     4681 atctttattg acgaaccatc tgtgaacctc agagttactc cataatcggg ggagtatgac
     4741 aaaattaccg cgagagataa aattcaggac aagcaaatca aactggcgta agtaatctgc
     4801 agcggtatgg cgatccggag gcaggggttc aatatgtggt gtatgcgctc ttttgatggg
     4861 aacgccaagg ttgatcattg gaaattgttc aataaaatca ttcagaaaca gaaatagcac
     4921 acgctcgacg ccggtctgaa atacgcgccc catagtgaca ggctttgggt ggggagcgca
     4981 aaaatattaa tttctccatc atggcgaaaa ttatctaaat aaatagggct taaatttatt
     5041 actacctgaa attcaatatt ttctttgtgt cttccattct tgacggaata agtgagttta
     5101 aattgaacct tttcattgta tagtgtgtcg ttaccccgaa aagaagtcct ctgaatatat
     5161 gataagttat caaggttgtt aaatatcttt tttgtggctg agttggtgtt taaagacgtg
     5221 cgccactctt catcacatat tatggtactg agtgcatcaa taaaatcctt taaaacttct
     5281 tttacctgag gtgcaatatt acgtacggga aaatcatcag gaagagatat gtaattactg
     5341 atgcttactg gcgtaaccat atttgcccca tatagtcata agtcatcgga acatcactat
     5401 atagggcatt tgagctgcaa gaactatcaa aaatagctca ttggtaaccc tgttttgccg
     5461 agtcaggcac atccagtagt tgaataaatg cctcaagcgg acgcgttttc gcaccacgac
     5521 gccagaccag ccaggttgtt aaccaacgcc attgctcagc taacggccac gcttcaacct
     5581 ggtgatgccc cggcatactt tccagcatag agcgtggaat aagcgcaatg cctgctcctg
     5641 cgaccacaca ggccaacatc ccgtgatacg actccatctc gtgaatagtt cccggagcgg
     5701 caccatcagc atgaaaccag ctctcgaagt ggcgacgata cgaacaattg gcgcggaagg
     5761 cataaatgtt gctgccgtta acctgactgg cgcggattac tggcgcatga ccttgtggcg
     5821 tgacgatcat cagctcctcg cggtataccg gtatcccgtc aatggcagta tggttaatgg
     5881 gcccatcaat aaatgccgca ttcagttttc cctccagcac tccttccagc atagtgccgg
     5941 aagggccggt ggaaagtgaa aactgaattt tgggataacg atggttgaat tccgccagcg
     6001 tggccggaat gcgcactgct gcggtgcttt ccagcgaacc aagagaaaat aaaccttgcg
     6061 gctcatcgcc agcgacaacg ctccgcgctt catccactaa cgcgagaatt tgttggctat
     6121 agcgtaaaaa gttatgcccg gcaggagaga gacgtaaacg ctgattctcg cgaataaaca
     6181 gatctacccc cagttctgtt tccagctggc gtagacgggt agtgagattc gacggcacgc
     6241 gatgcactat tgctgctgcc tgggttatgc tgccagcttc ggcaaccgcg ttgaacatct
     6301 ccagttgggt cagatccata atattctcga ttcgtgaata agtggcttaa tattattcat
     6361 tttaaagcaa gagtaaatct gcgtatcttc ataccatgac tcataaagga gataccccga
     6421 tgaccattac tccggcaact catgcaattt cgataaatcc tgccacgggt gaacaacttt
     6481 ctgtgctgcc gtgggctggc gctaacgata tcgaaaacgc acttcagctg gcggcagcag
     6541 gctttcgcga ctggcgcgag acaaatatag attatcgtgc tgaaaaactg cgtggtatcg
     6601 gtaaggctct gcgcgcccgt agcgaagaaa tggcgcaaat gatcacccgt gaaatgggca
     6661 aaccaatcaa tcaggcgcgc gctgaagtgg cgaaatcggc gaatttgtgt gactggtatg
     6721 cagaacatgg tccggcaatg ctgaaggcgg aacctacgct ggtggaaaat cagcaggcag
     6781 ttattgagta tcgaccgttg gggacgattc tggcgattat gccgtggaac tttccgttat
     6841 ggcaggtgat gcgtggcgcg gttcccatca ttcttgcagg taacggctac ttacttaaac
     6901 atgcgccgaa tgtgatgggc tgtgctcagc tcattgccca ggtgtttaaa gatgcgggaa
     6961 tcccgcaagg cgtatatggc tggctgaatg ccgacaacga cggtgtcagt caaatgatta
     7021 aagattcgcg cattgctgct gtcacggtga ccggaagtgt tcgtgcggga gcggctattg
     7081 gcgcacaggc tggagcggca ctgaaaaaat gcgtactgga actgggcggt tcggatccat
     7141 ttattgtgct taacgatgcc gatctggaac tggcggttaa agcggcggta gccggacgtt
     7201 atcagaatac cggacaggtt tgtgcagcgg caaaacgctt tattatcgaa gagggaattg
     7261 cttctgcatt taccgaacgt tttgtggcag ctgcggcagc cttgaaaatg ggcgatcccc
     7321 gtgatgaaga gaacgctctc ggaccaatgg ctcgttttga tttacgtgat gagctgcatc
     7381 atcaggtgga gaaaaccctg gcgcagggtg cgcgtttgtt actgggcggg gaaaagatgg
     7441 ctggggcagg taactactat ccgccaacgg ttctggcgaa tgttacccca gaaatgaccg
     7501 cgtttcggga agaaatgttt ggccctgttg cggcaatcac cgttgcgaaa gatgcagaac
     7561 atgcgctgga actggctaat gatagtgagt tcggcctttc agcgaccatt tttaccaccg
     7621 acgaaacaca ggccagacag atggcggcac gtctggaatg cggtggggtg tttatcaatg
     7681 gttattgtgc cagcgacgcg cgagtggcct ttggtggcgt gaaaaagagt ggctttggtc
     7741 gtgagctttc ccatttcggc ttacacgaat tctgtaatat ccagacggtg tggaaagacc
     7801 ggatctgacg cgacatattc agctctgata tactcgcagg tcttttcaga cctgcggtcc
     7861 aggagtagaa agtggcagtc gccatggata atgcaatttt agaaaacatc ttgcggcaag
     7921 tgcggccgct cattggtcag ggtaaagtcg cggattatat tccggcgctg gctacagtag
     7981 acggttcccg attggggatt gctatctgta ccgttgacgg acagcttttt caggccggag
     8041 acgcgcaaga acgtttttcc attcagtcta tttccaaagt gctgagtctc gttgtcgcca
     8101 tgcgtcatta ctccgaagag gaaatctggc aacgcgtcgg caaagatccg tctggatcac
     8161 cgttcaattc tttagtgcaa ctggaaatgg aacagggtat tccgcgtaat ccgttcatta
     8221 atgccggtgc gctggtggtc tgcgatatgt tgcaagggcg actaagcgca ccacggcaac
     8281 gtatgctgga agtcgtgcgc ggcttaagcg gtgtgtctga tatttcatac gatacggtgg
     8341 tagcgcgttc cgaatttgaa cattccgcgc gaaatgcggc tatcgcctgg ctgatgaagt
     8401 cgtttggcaa tttccatcat gacgtgacaa ccgttctgca aaactacttt cattactgcg
     8461 ctctgaaaat gagctgtgta gagctggccc ggacgtttgt ctttctggct aatcagggga
     8521 aagctattca tattgatgaa cctgtggtga cgccaatgca ggcgcggcaa attaacgcgc
     8581 tgatggcgac cagtggtatg taccagaacg cgggggagtt tgcctggcgg gtgggactac
     8641 cggcgaaatc tggcgttggt ggcggtattg tggcgattgt tccgcatgaa atggccatcg
     8701 cagtctggag tccggaactg gatgatgcag gtaactcgct tgcgggtatc gccgtccttg
     8761 aacaattgac gaaacagtta gggcgttcgg tttattaatg cagtctctcg atccactctt
     8821 cgcgcgttta tcccgttcaa aatttcgctc tcgctttcgt ctgggcatga aagagcgtca
     8881 gtattgcctg gagaaaggcg cgccagtcat cgaacaacat gcggcggatt ttgtcgctaa
     8941 acgtcttgct ccggcattac cggctaatga cggtaagcaa acccccatgc gcgggcatcc
     9001 ggtgtttatc gctcagcacg ccaccgcgac ctgctgtcgc ggttgtctgg ctaagtggca
     9061 taacattccg cagggtgttt cgttaagcga agaacaacaa cgttatatcg tggcggtaat
     9121 ctatcactgg ctggttgtac agatgaatca gccatgagaa ttgcggaaaa cgataaaata
     9181 gcattcccag ggtaccttta tttgccgccg ctgttagcga acatgctatc ctttatgtga
     9241 atttacttat tacgttgaga taattgccac ccgcaattgt atggacagat aattaatgca
     9301 tgtacacccc atttcaactt tccgtttgtt ccaggaaggt catctgctac gtaatagcat
     9361 cgctattttt gcgctaacca cgctgtttat tttattggtg cagagttacg gctggttcac
     9421 gaactttctc ttttctggcc gctgaatggc gtaatggcgg gggtgtttgc ccgctatgtc
     9481 tggcttaatc gactgcatta ctatgcgatc agttatgtgg cgatgctggt ttatgatgcc
     9541 ataaccaccg aatggggact ggtttcactg gttatcaatt tctccaatat gatgtttatt
     9601 gttaccgtcg ccttactggt cgtgcgggat aagcgtctgg ggaaaaataa gtatgagcca
     9661 gtaagtgcct tacggctatt taattactgt ctgattgccg cattattatg cgctattgtc
     9721 ggggcgattg gttcggtcag tattgatagt ctggatttct ggcctttgct tgcggactgg
     9781 ttcagtgagc aattctcaac gggcgtgttg atcgtgcctt gtatgctgac gttggctatt
     9841 cctggagtgc tgccgcgctt taaagcagaa cagataatgc ccgccatcgc gcttattgtg
     9901 tcggttattg cctcggtagt cattggcgga gcggggagtc tggcgttccc gctgcctgca
     9961 ttaatctggt gtgcagtgcg ctatacgccg caggtaacat gtctgttgac ctttgtcacc
    10021 ggtgcggtgg aaatcgtact ggtggcaaat tcggtgattg atatctcggt cggttcgccg
    10081 ttctccattc cggaaatgtt ctccgcacgt ctcggtattg ccacgatggc gatatgccca
    10141 attatggttt cttttagcgt ggcagcgatc aattcgctaa tgaagcaagt tgcgctgcga
    10201 gccgactttg attttctgac tcaggtttac tcacggtccg gtctttatga ggcgctgaaa
    10261 agtccatcgc tgaaacagac acaacatctg acggtcatgc tgcttgatat tgactatttc
    10321 aaaagcatta acgataacta tggacatgaa tgtggcgata aagtgttaag cgtgtttgcc
    10381 cggcatattc agaagattgt cggtgataag gggctggtgg cgcgcatggg cggcgaagaa
    10441 tttgctgttg cagtgccgtc ggtgaatcct gtcgatggtc tgctaatggc ggaaaaaatc
    10501 cgtaaaggcg ttgaactgca accattcacc tggcaacaaa aaacgcttta tctcacggta
    10561 agtattggcg tcggtagtgg tcgcgcatcg taccgaacgc tgaccgatga ttttaataaa
    10621 ttgatggtcg aagccgatac gtgtctgtat cgctcgaaga aagatgggcg caaccgtacc
    10681 agcactatgc gttacggtga ggaagttgtc tgacatcttt attcattccg cgcgatttga
    10741 atgcaatgtg ttctaccact ttatgatcca ggaaaaggcg tatttcctcg attaaaccat
    10801 gatccgcgcc acacttttct cataaaactg ctcattaatc atcaggagtt gatcaaaata
    10861 ggggttcggc gtattaaagt ttgctaacct gtcgtaccaa ttcaaacagg ttgtatgact
    10921 aatcagaagg gaacccattg tgaaaacact aaatcgtcgc gattttcccg gtgcacagta
    10981 tccagaacgt atcattcagt ttggtgaagg taacttcctg cgcgcctttg ttgactggca
    11041 aatcgatctc ctgaatgagc acaccgatct gaattctggt gtggtcgttg ttcgtccgat
    11101 tgaaacttca ttcccaccgt cactgagcac gcaggatggt ctgtatacca ccattatccg
    11161 tggcctgaat gagaaggggg aagcggtcag cgacgcgcgt ctgattcgct cggttaatcg
    11221 tgaaatcagc gtctacagtg aatacgatga attcctgaaa ctggctcaca acccggaaat
    11281 gcgctttgtt ttctctaata ccaccgaagc gggtatcagc taccatgcgg gcgataaatt
    11341 cgatgacgcg ccagcggtaa gttatccggc aaaactgacc cgtctgctgt tcgaacgttt
    11401 tagccatttc aacggtgcgc tggataaagg ttggatcatc atcccgtgtg agttgatcga
    11461 ctacaatggc gacgcgttgc gtgaactggt gctgcgctat gcgcaagagt gggcactgcc
    11521 agaagcattt attcaatggc tggatcaggc taacagcttc tgttctacgc tggtggaccg
    11581 tatcgttacc ggttatccgc gcgatgaagt ggctaaactg gaagaagagc tgggttatca
    11641 cgatggtttt ctcgacaccg ctgaacactt ttacctgttt gtgattcagg gaccgaaatc
    11701 cttagcgact gaactgcgtc tggacaaata tccgctcaac gtgctgattg ttgacgatat
    11761 taaaccgtac aaagaacgca aagtggcaat cctcaacggt gcgcacaccg cgctggtgcc
    11821 ggtggcgttc caggcagggc tggataccgt aggtgaagcg atgaacgatg ctgaaatttg
    11881 cgcattcgtt gaaaaagcta tctacgaaga aattattccg gtactggatt tgcctcgtga
    11941 tgaactggaa tctttcgcca gtgcagtcac cgggcgtttc cgtaacccgt acattaagca
    12001 tcagctgctg tctatcgcgc tcaacggtat gaccaagttc cgcactcgca tcctgccgca
    12061 gctgctggca gggcagaagg caaaaggcac acttccggcg cgtcttactt tcgcattagc
    12121 ggcattgatt gccttctatc gtggtgagcg taacggagaa acatatccgg tacaggacga
    12181 tgcacactgg ctggaacgtt atcagcaact ctggagccag catcgtgacc gtgttatcgg
    12241 tacccaggaa ctggtagcga ttgtgttggc agaaaaagac cactgggagc aggacctgac
    12301 gcaagtgcct ggtctggtcg agcaggttgc taatgacctg gatgcgattc ttgaaaaagg
    12361 gatgcgcgaa gctgtacgtc cgttgtgcta ataacaccgt tacttctgaa cccggtttcg
    12421 accgggtttc ttatttcact atattagtta caacatccta tgtgccttta tcattaacag
    12481 gtgccacatg cagcgcctgt taatgttgta agcttgtagg taatttgtaa gtagcgtctt
    12541 gagaatgtga tcataatcac gtttaatagg gcgacttttt cacgcctgaa gataaccatg
    12601 attgttcgtc cacaacaaca ctggctgcgc cgtatttttg tctggcacgg ctcagtatta
    12661 tccaagatat catcgcgctt actactcaat tttctctttt ctatcgctgt tattttcatg
    12721 ctgccctggt acacgcatct gggcatcaaa ttcaccctcg caccgttcag cattctcggt
    12781 gtcgccatcg ccatttttct tggttttcgt aataatgccg ggtacgcccg ttacgttgaa
    12841 gcgcgaaaac tttgggggca gttgatgatt gcctcacggt cgttactgcg cgaggtaaaa
    12901 acgacattgc cggattcggc aagtgtaagg gagtttgccc ggttgcaaat cgccttcgcc
    12961 cactgtttac gcatgacatt acgcaaacag ccacaggcgg aagtgctggc tcattatctc
    13021 aagactgaag atcttcagcc tgtactggct tcgaactctc cagctaaccg tatcttgtta
    13081 ataatggggg agtggttggc ggttcagcgc cgcaatgggc agctttcaga tatcctgttt
    13141 attagcctca acgatcggct taatgatatt tcagcggtcc tggcaggatg cgagcgcatt
    13201 gcctatacgc caattccctt tgcctacacc ctgattttgc atcgtaccgt ttatctgttt
    13261 tgtatcatgc tgccgttcgc gctggtcgtg gacctgcatt acatgacgcc ttttatctct
    13321 gtgctgattt cctacacttt tatttcgctg gattgtctgg cggaagaact ggaagatccg
    13381 ttcggtactg aaaacaatga tttaccgctg gatgccatct gcaacgctat tgaaattgac
    13441 ctgttacaga tgaacgatga agccgaaatt ccagcaaaaa ttcttcccga tcgtcattac
    13501 cagctgacgt gatatttact ccgtacggcg ggcaacaata aaaagacgcg gaaatgccag
    13561 cagtatctgt ccgttctctt gcagtggata ctgctcttcc agcatctgat ggtagcgctt
    13621 aagaaaaagc tgctgttcgc tctcggtcag atcctgtaac cacggacgta atccagtggc
    13681 ggtcacccaa tcgataatcg cctggtgtga cggcatctgg tgatagtagg tggttcgcca
    13741 gatatcgacc tcacatccgg cttcgctcaa aatatcgtag taagcatgaa cgccagccaa
    13801 cggctcacgc ccacgatccg ggtagttttg ttcccaggca acttcgcgca tgagcacatg
    13861 ggtcggctcc agccagttat ctggcatctg tactgccagc acgccctgcg gattaagtaa
    13921 agaaaccaga tgaggaaaca attcgtagtg gtcgggcagc cattgcagtg aggcattagc
    13981 aaaaatcaga tcgagtgcct gtaccggttg ccagttgcgg atgtctgctt ccacgaactg
    14041 gcagtctggc aaagcactgc gcgcttcagc aatcatcgcc ggagacgagt cgatgcccgt
    14101 tatcctggca gcaggccaac gttgttgtag aagggcggtg ctgttacctg ggccacagcc
    14161 aagatcggca acatattcga cattctccag cggcactctg gcaagcagct ccaccgccgg
    14221 acgcgatcgt tcagcggcaa agtgcagata taaagagggg ttccagtcag acattttttt
    14281 actcccgtca gcgcgttacc ccaattgtag ataaaattga taaattcgcc tcacggcatc
    14341 aacccattga acagacgttt tttacgcggc ccggtcatta aagattccag tttcgcgaca
    14401 caggttttgt agtggggcgt ggttttatgg aatgccactg cgtcttcatc tttataggct
    14461 tcgtagatat aaaagcgcga attcacttcc gggtcctgta agacatcgaa gcgcaaattg
    14521 ccttcttcct gtaccgagcc caggtggttc tggcgaaaaa cttcgataaa ctcgtcaacc
    14581 ttgtcttcat gaacgttaat ttcaaccagt gtgacgtgca taattcttct ccttaaatcc
    14641 gcagttactg tttttcactc agatagagtt catatgcccg atcagccgtt tcgttatggt
    14701 gaaccaccgc ctgtacggct ttcatcatcg ccaccggatg gtcagactgg aaaatattac
    14761 gccccatatc cacaccagaa gcgccctgat cgatagcctg ccagcacatt tccagcgcct
    14821 cgcgctccgg taatttttta ccgccagcaa taacaatggg taccggacat ccggcaacaa
    14881 tccgttcaaa acctttttcg acataatagg ttttgataat ttgcgccccc atttcagcgg
    14941 cgattcgagt cgcgagcgag aaataacgct gatcgcgcac catatctttg cccacgccag
    15001 tcacggccat ggtcggcatt cccactttca ttccggcatc gaccagctga ataatatttt
    15061 tgatcgactg atgttcatat tcgctgccga tataaacctg cgccgccacc gcgcaactgt
    15121 tcaggcgcac ggcatcatcc atcgataacg ccacggcttc attacttaat tccgccagaa
    15181 tagagttcgc acctgacgcc cgcagtacca ccggcttatt ggtcgccggg ggaactacgc
    15241 tgcgcaaaat gccgcgcgta cacattaata catcggcatg ttcaaacagc ggggcgatat
    15301 ttatatcaat gcgttcaagt ccggtagtcg gtccctgaaa ataaccatgg tcaaaagcca
    15361 gcatcacggt tttacccgtt ttcggattaa atatccgcga taagcgtgac tgcattcccc
    15421 aatccagcgc accgcaacct ttcagggtaa aagggatatt ttgttgcggt tgatcggtac
    15481 gaaaatcttt accatcttta atatcgtcta aatctgccat aacttactcc ggttaagtaa
    15541 tgcacatcag aaatcgtatt tgccgatatt ctctttgttg aatatcacgc gctccggtaa
    15601 cagtacgatg ccattaccat ccgcttcgta gtcatagccc tgaacgctgt ttggcgagac
    15661 ttccacctga cctactccct ggatatccag cttgtcgccc gttttcattg atcctttttt
    15721 caataatgca tccgcgacat acactgaaat tttgccttgc tgaaccacat cccacaggcc
    15781 aaattctttc accgtgccgc gctctacata cgggcgcatc acatttggcg tactgaatcc
    15841 gacaatcgct actttgtcat ttttcaggtt ttctgcggct tgtgcggcag cgggcagggc
    15901 gttggcatcg gggacgataa tggcgtcgag atcgctatac gcttttaata ttccttctgc
    15961 tgtttgtaac gatttagtgg catcgttata gccaaactgc gtagtgacaa tttcccagcc
    16021 tggatgctct ttggcgattt tcgctttcgc ttctttcacc cactggtttt ggtccgtaac
    16081 ggtggggctt gagtagaaaa acgcgacttt ggctttgtct ttattcacct gacgcgccgc
    16141 catatccacc aacatacctc ccaactgggc gggcgttccc tgattaatgt agtaagagcg
    16201 gcactccggt ttagtatcag agtcccaggt cagcactctc acaccacgtt gcatggcgcg
    16261 tttcagtgcc ggacacaagc catcaggcga aaccgcagaa acgataatgg cgttataacc
    16321 ttgattgacg aagttattaa tcaactgtac ctgaccagaa acactgggtt ctgtcggccc
    16381 gtcgtaggtc acatcaacgc ccagctcttt acccgcttgt tgtgcgccgt tgccaccgct
    16441 ggtaaaaaat cccacgccaa ccagtttggg aataaatgca atacgctctg cggcctgcac
    16501 attcatagag attgcggcaa tgccaagagc gctaagtaag gcgattttct taaagcgatg
    16561 aagtgtcatg aagatatcct ttatggcaat gggttattgg cccgacgcgc cagccactct
    16621 ttaatttgct ggcgatgcag gctaacggaa cgacctacga caacgacgat aagtagcgca
    16681 ccggaaaggg cgctggacac ctgatttggc actcctgcca tttgcaaacc ttgttgcaaa
    16741 tatcccacta ataaaaccgc aatggcggtg ccgataatgg aaccggaacc accataaata
    16801 ttggcaccgc caagcaccac ggcggtgatg gcgggcatca gaaacgacgc accgagatcg
    16861 gaacgtgctg aaccaaaata cgacaccagc agcacagcgg cgaccgcaga cgccagcccc
    16921 gtcatggcat agagcgcaca taaggtgcgg ttaactggaa tcgcgctata aagcgccacg
    16981 cgcgggcttt gcccaatcaa aaacacatta cgtccggcat gggttttatg cagccagagc
    17041 cagaaaacga ggagacatat caggaagata atcagcggaa cggggagtcc cagcacatcc
    17101 aggttagcga aatctgtaaa cgccatcggg aatccaccaa taccttcgta ccccgtcgct
    17161 ccggccatcc cggaaagcag tagggcgctt ccggcaaaca gatacagcgt gccaagcgta
    17221 atcaccagcg ggttaacttt ggtatagata attaatccgg cgttgatcag cccgcacaat
    17281 gcgccgagca gtaaggtcag gagaatcgcc agcggcatcg gcacaccact ttgaaacagt
    17341 acgcccaatg caatggcgca gaggccgatg gtcgaaccaa acgaaatatc gatcccgcca
    17401 ctgacaatca ccatcgtcag cggtagggcg acaatgccaa tgcagataaa gtcactggtg
    17461 ctgaacagca acatattgag atctaacatt cgcgggttaa ttgcaccaaa tgcgacaatc
    17521 tcaataacga gcagtgcggc aagagccagt tcccaaccgt agcgaatacg cattatgcgg
    17581 cctcccgttt tttacctgac gaagcgggtt taacggatgg cggtggcgtc ataaagcggg
    17641 catatttttg ccgccgtaga ttacgttcca gcgcacaacg caggcgtcca tcaaacacca
    17701 gcaccgccag cagcacgagg cccgcgataa aatcgttcca ccatgccgga atgcgcaaca
    17761 gcaccagtac gctatcgatc tgcgtcagga accatgcgcc gagtaccgca ccgatgatcg
    17821 caccggaacc gccgagcaaa ctaatgccgc ccagcacgca ggctgcaatt gctttcatct
    17881 ccagcccggt accggtctgg ttggggataa aaccaatctg cgaagcaaac acaattcccg
    17941 ccagtgccgc catgcagccg ttcaacgaaa atgccacaat gcgaatggct tccgtacgaa
    18001 cacccagttg acgagcgccc tgtaaattat cgcccgtggc ataaaaactg cgtccaaacg
    18061 ccgtctttgc cagcagccag gccataaatg ccaccagaat tatcgtcaac caaccaatcg
    18121 cggaaacgcc aaacagcagc ggtgcggaga gctgtttcag ttcggcgggt aacccttcaa
    18181 tccatttacc gccagtccac agcaacatga tgcctctgta caaccctaac gtgccaaggg
    18241 tggcaacaat ggcagggatt tttagccacg cgaccaggac accgttgaaa aatcccgcga
    18301 gcaaaccaag cagtaaagtc gcgacacaag caacaggtag tgaatacccc gcgttcagta
    18361 acatccccaa cagcactgcg cacattccgg taatcgaacc cactgaaaca tcaatattgc
    18421 gcgtaagcat taccagcgtc gcgcccattg ccagcaggat caggatttgc gcgctgctat
    18481 aaaccatggt cagcgtttgc acacttaaat actggcggtc gagaaaaccg ggtaatgcaa
    18541 acagcagcac caccgccagc agtgccgtga tttcacggtt gttctgaata aacttcagca
    18601 tgatgcctcc tgacgctgac tatcgccgaa ggcaacgcgc ataatagtct cgacattaat
    18661 atcgcgcccg gtcagtgcag agtgggtaat ttcgccctga tgcatcacat acacacgatc
    18721 tgtcatcagt tcgatctctt ccagatcaga ggagataagc agcacagcca cattctgtgc
    18781 ggcgatgctg cgcaacagct ggtagatatc attacgtgcc gagacatcca cgccgcgcgt
    18841 cggctcatcg acaatcaata cttgcggcga agcttccagg catttggcaa tgaggatttt
    18901 ttgctggttg ccaccggata atgtccgtgc agcttgttcc ggttggttaa atttaatatt
    18961 cagcgcccga cgataacgtt ccagggtggc attatctttc gcggttttcg cccagaatcc
    19021 acgaaggtta tgagtaaggg cgcagacgtt ccaggccagc gaagcatcga gattcagtcc
    19081 ggatgactgg cgatcttccg gcagataaac cagaccgcgc agtaaacgtt ctccagtgga
    19141 taatttattg atctctttac cattcagcat aatgcgtccg ccacgcaaag tacgcagacc
    19201 atagagcgtc tcggccagtt ctgtgcgtcc ggctcccacc agcccagcca ggcccagaat
    19261 ttctccggca ttgagcgtca ggctgacatt cctgaaacct tcgccggtca gattttccag
    19321 tgtcagcacc ggcgttccgg cagcatgttg tgggcggtta ccaggtaact ccagccataa
    19381 tttttggctg gcagagagcg atttttcccg taccgctggg gtgatggcct gaataatgtc
    19441 gtcggtagac agttcgctgg ttttgccgct taaggcgatg gttccgtcgc gcatcacgct
    19501 aattcgatcg gcaatctggc gaatttccgg cagcttatgc gagataaaaa caatacccac
    19561 gccagtagca agcagctctt gcaagcgact aaacaagcgt tcggtttccg caggggtaag
    19621 cgaggcggta ggttcatcga ggatcagaat ccgcgagtcg cgcatcagcc cgcggaggat
    19681 ttccaccatt tggcgatcgg cgacatccag cgatcctgcc agactatgca gatcaaactg
    19741 gcagcccagc gccgccagca agttcttcat tttttgcatg gagagctgtt tttttgccat
    19801 cccaaacagg atgttttctt ttatcgacag gcttgggaaa agcagcggtt cctggggaac
    19861 gagataaatg cccagctgat gagcatgaac tggcgttaat ctggcgtagt tgttgccccc
    19921 aatctccagc gtaccgctat cagcaggggt aataccggca ataatcttca ttaacgtcga
    19981 tttaccggca ccattgccgc cgagcagggc gtggacctcc ccctgatgca acgtaaaatc
    20041 gatgcctttc aggacattga cccctgaata ctgtttataa accgagcggg cgcaaagtag
    20101 cggtaacgcg cgggtatcac tcgtttgcat atttcccccg ttcagttttg caggtgagtt
    20161 ttgaacaaat gtatttctgc ttttaatttg ttcataacct taggtggaca ttgcacatat
    20221 ttccgacgaa tagatcacaa tttatgctat tttgattttc acggttgcgt ttgttcatgc
    20281 tcgtagagtc aaactgtggc tgccatcaca gatataaatg cgcaagaact gaacaattgc
    20341 attaaagatt taaatatgtt caaagtgaag aatgaattat gacaatcaac gattcggcaa
    20401 tttcagaaca gggaatgtgt gaagaagaac aggtcgcgcg gatcgcgtgg ttttactatc
    20461 acgacgggct gacccagagc gagatcagcg atcgtctcgg cctgacccgt ttgaaagtgt
    20521 cgcgcttgct ggagaaaggg catcagtccg gcattattcg cgtacagatt aattctcgct
    20581 ttgaaggctg tctggaatat gaaactcaat tacgtcgtca gttttcgctg caacatgtcc
    20641 gggtgatccc tgggcttgcg gatgctgatg tcggcgggcg actggggata ggcgcggcgc
    20701 atatgttgat gagtttactt caaccacaac agatgctggc gattggtttt ggcgaggcaa
    20761 ccatgaatac gctgcaacgc ttaagtggtt ttatttcgtc acagcaaatt cgcctggtca
    20821 cgctctccgg tggcgtcggt tcttatatga cgggtatcgg gcagcttaac gcggcgtgca
    20881 gtgtgaatat tattccggct ccgttgcggg catcctccgc tgacattgcc cgtacgctaa
    20941 aaaatgaaaa ttgcgtcaaa gatgttctgt tagccgcgca agcagccgat gtggcgattg
    21001 tcggcattgg ggccgtcagt caacaggacg acgcgacgat cattcgctcc ggttatatca
    21061 gccagggcga acagttaatg attggtcgaa aaggggcggt tggcgacatt ttaggctact
    21121 tttttgatgc aaaaggtgac gttgtcacgg atattaaaat acataacgaa ctgattggct
    21181 tacctttaag ctcgctgaag accatacccg tccgggttgg cgtggcaggg ggagaaaata
    21241 aagccgaagc aattgccgct gcaatgaaag gcggttatat caacgcactg gttaccgatc
    21301 aggacacagc agcggcgatt ttacgtagtt aaatttgcat gaccttatta ccgggaatga
    21361 tggagaacgt atagaggaac agacagaacg cataagccga ggataatcta atggctcgac
    21421 tctttacccc ttcagaatca aagtattacc tgatggcgct ggatgcaggt accggaagta
    21481 ttcgggctgt gatattcgac ctggaaggca atcaaatagc agtgggacag gcggagtggc
    21541 ggcacctggc agtaccggac gttcctggtt ctatggaatt tgatctcaac aaaaactggc
    21601 aactggcgtg tgagtgtatg cgccaggcgc tgcacaacgc cggcatagcc ccggagtata
    21661 tcgctgccgt ttcggcatgt tcgatgcgtg aaggcattgt tttatataat aatgaaggag
    21721 ccccgatctg ggcctgcgcc aatgtggatg ccagagcggc acgcgaagtt agcgaactta
    21781 aagaactgca caacaatacc tttgaaaacg aagtttatcg cgcgaccgga caaacactgg
    21841 ctttaagtgc catccccaga ttactttggc tggcgcacca tcgttccgat atttaccgtc
    21901 aggcatcaac catcaccatg atcagcgact ggctggccta tatgctcagc ggcgaactgg
    21961 cggtggatcc ctctaacgct ggcaccacgg gacttcttga tctaaccacc cgtgactgga
    22021 aacctgcatt gctggatatg gctggcctac gtgccgatat tctttctcct gtcaaagaaa
    22081 ccggcacatt gctgggcgtg gtaagttcac aagcggcgga actctgcggt ctgaaggcgg
    22141 gcactccggt ggtcgttgga ggaggcgacg tgcagcttgg ttgccttggg ttaggcgttg
    22201 tgcgtccggc acaaaccgcg gttcttggcg gcacattctg gcagcaagtt gtaaatttag
    22261 ccgcgccggt gacagaccca gaaatgaacg tgcgcgttaa tcctcatgtt attcctggca
    22321 tggtacaagc tgaatctata agctttttta ccggactcac catgcgctgg ttccgcgatg
    22381 ctttctgtgc cgaagaaaaa ctgattgccg aacgtttagg catcgacatc tatacgctgc
    22441 tggaagagat ggccagtcgg gtgccgcctg ggtcgtgggg cgtaatgccg atcttctccg
    22501 acagaatgcg ctttaaaacc tggtatcacg ctgcgccttc ctttattaac ttgtccattg
    22561 acccggataa atgtaacaaa gcgacattgt tccgtgcgct ggaagaaaat gcggcgattg
    22621 tatcagcgtg taacttgcag caaattgctg atttctcgaa tattcatcct tcatcgttag
    22681 tctttgcagg cggaggttca aaagggaaat tatggagtca aattctcgct gatgtctcgg
    22741 gattacccgt caatattccg gtggtcaaag aagccactgc attaggatgt gccattgcag
    22801 ctggcgtcgg tgccggaatt ttttcatcaa tggcagaaac cggagaacgc ctggttcgct
    22861 gggaacggac gcacacacca gacccggaaa agcatgaact ttatcaggat tcacgcgata
    22921 agtggcaggc agtttatcag gatcagctgg ggctggttga tcatggactg acgacgtcgt
    22981 tatggaaagc gcctgggtta tagtttcatt gacagccaca tcatgatgac cgtggctgtt
    23041 agtttttgag tatagttatt cttatttccc ttcttttaga gctggcttgt taaaagccag
    23101 cttgcagatt catcgttcca tcaccagtac tatattcaag ggaaaattgc tatttttctt
    23161 aaagcattaa gcacaaccct tattttatag cgaatattcc taaagacact aatggatgca
    23221 aaaggggttt aataaacttc gatttttagc aattagtgct attctttgtt aaatatgatg
    23281 atgataattt tcctattgaa agaaacagtt ttcttattaa gcaacaataa ctcatagttc
    23341 atgtttaaat aaaataagaa ctatgcaatt tttaatctat caattcgaaa gcattagcaa
    23401 attagccaaa ttgacacctt tccacctcca actaacactc aatacagacc tctgacaatt
    23461 taataagttt catttttaat gtctgacgac tcatctcttt cgtggctgta ttgtttgtat
    23521 atggagcgtt tatatgaata gaatctatcg cgtgatatgg aattgcactc tacaggtatt
    23581 tcaggcctgc tcggaattaa ctcgcagggc aggtaaaaca tcgacggtta atttgcgtaa
    23641 atcctctgga ctgacaacga aattcagtag attgacgctg ggtgttttgc tggcactaag
    23701 cggttcagcg tctggtgcaa gtctggaagt tgataatgat cagattacca atattgatac
    23761 tgatgttgct tatgatgcct acctggttgg ctggtatggc actggagtgc ttaatatttt
    23821 ggctggcggt aatgcctcct taaccactat tactaccagc gtcattggcg ctaatgagga
    23881 ctcagagggc accgttaatg ttttgggtgg cacctggcga ttgtatgata gcggaaataa
    23941 tgcaaggcct ttaaatgtgg gtcaatccgg aacggggacg ctgaatatta aacagaaggg
    24001 tcacgtcgat ggaggctatt taagattagg ttcttcgaca ggaggcgtcg ggacggtcaa
    24061 tgttgaggga gaggactctg ttctgacgac cgaattattc gaaataggga gttacggtac
    24121 gggctcatta aatattacgg ataagggtta cgtcacgagt tcaatcgtcg ccattttagg
    24181 ctatcaggcg ggcagtaatg gccaggttgt cgttgaaaag ggtggcgagt ggctaataaa
    24241 aaataacgat tcctcaattg aatttcaaat tggtaatcaa gggatggggg aagcgactat
    24301 tcgcgagggg gggctggtta cggctgaaaa tacgattatc ggtggcaatg ccaccggtgt
    24361 cggaaccctg aatgtgcagg atcaagactc tgtcatcacg gtacgcagac tctataatgg
    24421 atatttcggt aatggcgcag tcaatatttc caataatgga ctaattaata acaaagaata
    24481 ttcattggtg ggcgttgaag acggttccca cggtgtcgtc aacgtgaccg ataaagggca
    24541 ttggagtttc ctcggaacag gcgaagcttt ccgctatatc tatatcggtg atgctggtga
    24601 cggtgaactt aatgtctcgc gtgaaggtaa agtagattcg ggaattatca ctgcggggat
    24661 gaaagaaaca ggcacaggca acattactgt taaggataag aactccgtta tcactaatct
    24721 cggaactaat cttggttatg acggccacgg cgaaatgaat atcagtaatg aggggcttgt
    24781 tgtcagcaac ggaggaagct cactcggtta tggagaaaac ggcgtcggga atgtcagcat
    24841 caccacgggg gggatgtggg aggtcaataa gaatgtctat accaccattg gtgttgcggg
    24901 cgtcggaaac ctcaatatta gcgatggcgg taagtttgta tcgcaaaata ttactttttt
    24961 gggcgataaa gcaagcggta tcggcacact gaacctgatg gatgggacat catcgttcga
    25021 tactgtgggt atcaatgtcg gtaattttgg tagcggtatc gtaaatgtca gtaatggtgc
    25081 cacccttaat tcaacgggct atggatttat cggaggaaat gcctccggta aagggatagt
    25141 taatatttca acggatagtc tctggaattt aaagacatca tctactaacg cccaattgct
    25201 acaggtcggt gtattaggca cgggtgaact gaatattacc accggaggta tagttaaagc
    25261 gcgtgataca cagatagctc tcaatgacaa aagtaagggc gacgtgaggg tggatgggca
    25321 gaactctctt cttgaaacat tcaatatgta cgtagggaca tctggtacgg gtacgttaac
    25381 cctgacgaat agcggcacgc tgaatgtcga aggtggagaa gtttacttag gtgtttttga
    25441 acctgctgta ggaacgctaa acattggtgc agctcacggt gaggcggcgg cagatgccgg
    25501 gtatataacc aacgcgacga aagtagagtt tggttctggc gaaggtgttt ttgtctttaa
    25561 tcatactaat aacagtgatg ccggctacca ggtcgatatg ctgattacgg gtgacgataa
    25621 agacggaaaa gtgatccatg atgcaggcca tacggtgttc aatgcaggga atacttatag
    25681 cggtaaaacg ctggtcaatg acggcctcct gaccattgcg tctcatacgg cagatggggt
    25741 aacgggcatg ggttcgagtg aagtcaccat tgcaagcccc ggtacgctcg acattctcgc
    25801 atcaacgaac agtgcaggag attacacgct gaccaatgcg ctcaaaggcg atggtttgat
    25861 gcgagtgcag ctgtcatcct ccgacaagat gtttggcttt acccatgcaa cagggactga
    25921 attcgccggt gttgcccaac tgaaagacag tacct
//
LOCUS       DAMZET010000053        25075 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.053, whole genome shotgun sequence.
ACCESSION   DAMZET010000053 DAMZET010000000
VERSION     DAMZET010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.053"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..726)
                     /gene="feaB"
                     /locus_tag="POB34_002212"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(<1..726)
                     /gene="feaB"
                     /locus_tag="POB34_002212"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HDI5919149.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHDLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPH"
     gene            962..1867
                     /gene="feaR"
                     /locus_tag="POB34_002213"
     CDS             962..1867
                     /gene="feaR"
                     /locus_tag="POB34_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HDI5919150.1"
                     /translation="MNPAMDNEFQQWLSQINQVCGNFTGRLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESIN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(2039..2368)
                     /locus_tag="POB34_002214"
     CDS             complement(2039..2368)
                     /locus_tag="POB34_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HDI5919151.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(2373..2558)
                     /locus_tag="POB34_002215"
     CDS             complement(2373..2558)
                     /locus_tag="POB34_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HDI5919152.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(2555..5203)
                     /locus_tag="POB34_002216"
     CDS             complement(2555..5203)
                     /locus_tag="POB34_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HDI5919153.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQEWQGKLSLALTSDIQQLRY
                     QGEKVKFQGQLKGQQLTVSELDVVAFENQSPVKLVGEFTMPLVPDGLPVSGHATATLN
                     LPQEPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGF
                     PLSGRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQ
                     LTGEAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAG
                     VKVTQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMN
                     ATWDVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFS
                     GALSLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGI
                     RLRGNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGG
                     SAWMPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYP
                     WTEEEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMS
                     GAFSGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYM
                     EISRSSTNINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDN
                     LQAWLEQNARLPGNDCPQGKECEEKQ"
     gene            5411..6400
                     /gene="ldhA"
                     /locus_tag="POB34_002217"
     CDS             5411..6400
                     /gene="ldhA"
                     /locus_tag="POB34_002217"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDI5919154.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            6511..6933
                     /gene="hslJ"
                     /locus_tag="POB34_002218"
     CDS             6511..6933
                     /gene="hslJ"
                     /locus_tag="POB34_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HDI5919155.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNQPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(6930..7196)
                     /locus_tag="POB34_002219"
     CDS             complement(6930..7196)
                     /locus_tag="POB34_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HDI5919156.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            7470..10994
                     /gene="nifJ"
                     /locus_tag="POB34_002220"
     CDS             7470..10994
                     /gene="nifJ"
                     /locus_tag="POB34_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDI5919157.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLIRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGSWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSVALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            11362..12495
                     /gene="ompN"
                     /locus_tag="POB34_002221"
     CDS             11362..12495
                     /gene="ompN"
                     /locus_tag="POB34_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HDI5919158.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQRYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            12636..13070
                     /gene="uspF"
                     /locus_tag="POB34_002222"
     CDS             12636..13070
                     /gene="uspF"
                     /locus_tag="POB34_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HDI5919159.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEM
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            13246..14181
                     /gene="ttcA"
                     /locus_tag="POB34_002223"
     CDS             13246..14181
                     /gene="ttcA"
                     /locus_tag="POB34_002223"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDI5919160.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(14310..15683)
                     /gene="dbpA"
                     /locus_tag="POB34_002224"
     CDS             complement(14310..15683)
                     /gene="dbpA"
                     /locus_tag="POB34_002224"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HDI5919161.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATLGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSILPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGVDIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(15713..15886)
                     /locus_tag="POB34_002225"
     CDS             complement(15713..15886)
                     /locus_tag="POB34_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017372341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919162.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(16161..17144)
                     /gene="zntB"
                     /locus_tag="POB34_002226"
     CDS             complement(16161..17144)
                     /gene="zntB"
                     /locus_tag="POB34_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HDI5919163.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHESAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            17399..18631
                     /gene="dgcM"
                     /locus_tag="POB34_002227"
     CDS             17399..18631
                     /gene="dgcM"
                     /locus_tag="POB34_002227"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HDI5919164.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(18652..19215)
                     /gene="smrA"
                     /locus_tag="POB34_002228"
     CDS             complement(18652..19215)
                     /gene="smrA"
                     /locus_tag="POB34_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HDI5919165.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(19545..20453)
                     /locus_tag="POB34_002229"
     CDS             complement(19545..20453)
                     /locus_tag="POB34_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI5919166.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            20629..21939
                     /gene="abgA"
                     /locus_tag="POB34_002230"
     CDS             20629..21939
                     /gene="abgA"
                     /locus_tag="POB34_002230"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HDI5919167.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRTQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            21939..23384
                     /gene="abgB"
                     /locus_tag="POB34_002231"
     CDS             21939..23384
                     /gene="abgB"
                     /locus_tag="POB34_002231"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HDI5919168.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESASFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWCFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKVMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            23421..24947
                     /gene="abgT"
                     /locus_tag="POB34_002232"
     CDS             23421..24947
                     /gene="abgT"
                     /locus_tag="POB34_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HDI5919169.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            24958..>25075
                     /locus_tag="POB34_002233"
     CDS             24958..>25075
                     /locus_tag="POB34_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--protein-cysteine
                     methyltransferase"
                     /protein_id="HDI5919170.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMV"
BASE COUNT         6301 a         6380 c         6138 g         6256 t
ORIGIN      
        1 atgaggatgt gacgtcaggg ccgcgccgca tacagcacct gacccggtga cgacattaaa
       61 aacgccatca gggataccag cctcgctggc cagttccgcc acgcgcaaca tcgtcagtgg
      121 cgtggtttcc gaaggcttaa tcacgattga acagcctgct gccagtgctg gcatcacctt
      181 ccacatacca atcatcaacg gaaagttcca tggcacaatt cccgccacta cgccaaccgg
      241 ctctttacgc gtccaggcct gataacgcgc cccctggggt aagggaatcg acaagtccag
      301 cgttttaccc gcgattttgg tcgttaaccc ggcggtataa cgcatccagt tcagcgtaca
      361 gcccacttca aaagcacggg aaatggcaat tgacttgcct tgctccaggg tttccagttg
      421 cgccagctcc tcactgtgct gttccaccag atcggcaaaa cgtagcagaa tacgttcacg
      481 ctctgcgggt aatcgcccgg cccagcgacg cgagacaaag gcccgccagg cagacatgac
      541 tgcgttatct acatccgctt cgttggcatc agcagtagac gcaatttctt gcccggtggc
      601 cggatcaaag atcgccaacc gtttttcact ttgtgcgggg ccaggacgac catcaatata
      661 aagatcgtgt tgacgatcga gaaactgttg gacctggctt aatactgcta catgcggctc
      721 tgtcatcact tttccttatt atttacccag tgtgatttca gtgtacgcag cgacaagaaa
      781 agtcactttt ttcgctgtga cattcgcttt gtcagctgtg acaagctccg caaatcgtga
      841 caataaccgc ttatgtctct atgcctgaca agacttattt cctatagtca ggctcattag
      901 cttcgtttat tgcaacacaa atgcaacaat aaaaatacat ttcacagagc gaaagcgtgc
      961 catgaacccc gcaatggata atgagtttca gcaatggctt tcccaaatca atcaggtatg
     1021 cggaaatttt accggacgcc tgctgactga gcgttacacg ggtgtactgg acacgcattt
     1081 tgccaaagga ctaaagctga gtaccgtgac aaccagcggg gtgaatttat cccgcacctg
     1141 gcaggaggta aaaggcagcg acgatgcctg gttttacacc gtttttcagc ttagtggtca
     1201 ggcaataatg gagcaggatg agcgtcaggt gcagattggc gctggcgata ttacgttact
     1261 cgatgcctca cgcccctgtt cgctttactg gcaggagtct tctaaacaga tttcattact
     1321 tttgccacgc actctgctgg aacaatattt tccccatcaa aaacctgtct gcgcagaaag
     1381 actggacgct gacttaccca tggtgcaact cagtcatcgc ctgttacagg agagcataaa
     1441 taatccggca ctttctgaaa cagaaagtga agctgcgcta caggcgatgg tgtgtctgct
     1501 gcgcccggta cttcatcagc gggaatctgt tcaacctcgt cgtgaacgtc agtttcaaaa
     1561 agtggttacg ttgatagacg ataatattcg cgaagagata ttacgcccgg agtggatagc
     1621 cggagagaca ggtatgtcag tacgtagttt gtaccgaatg tttgccgata aaggtttggt
     1681 ggtcgcgcaa tatattcgta accgtcgtct cgatttttgt gcagatgcga ttcgccatgc
     1741 cgcagatgat gaaaaactgg caggcatcgg ctttcattgg ggattttctg accagagtca
     1801 tttttcaacg gtatttaagc aacgctttgg gatgacgcca ggcgagtatc gacgtaaatt
     1861 ccgctaacta attggcgttg tgtgtactgc aacgccaatt agttaaaaca gatatttgac
     1921 tatgcatttt tcagggcgaa aggcaatttg acaggagttt ttgaatattc accttgtttc
     1981 aggaaaatag atcaatcgct ttctcctgta gcgttatccc gatattgcgg ttttgctatt
     2041 aaaattttcg cacccattta ccgtttatcc cttgtacata ttcccccggt ttggcccgag
     2101 ctacgagctt ttgcccggcg agtttcgcga tatcatctac agacacatta ttttgctttg
     2161 ccagttgttg gtaactcgct ttacgttcgg cattaatgtc ggttactaat ttctctgttt
     2221 cagcatccgt tttcagcgca accagataac cataaaatgt ttcacctacc cgcccctgag
     2281 ttctggcttc atccagagtc aatgccatta cattgctgct taccagccca acaagaaacg
     2341 cacaaagaag taatgttttc ttcatcatcg cctcagaaaa gatcgctacg agtttcaagc
     2401 agctcttcga catctttgtc tgccttgatg atgatctcat gctcaatttt aacgttcata
     2461 ttgatagtga tcggttcctt gggcgcagcg acttcaatac gcggcgtaca ggcagccagc
     2521 ataaaagatg acgtcaacgc agccagtaaa attttcattg tttttcctca cactcttttc
     2581 cttgcggaca gtcatttccc ggcagacgtg cgttctgctc caaccatgcc tggagattat
     2641 cgccaaagcg taaactgcgc cacagcgtaa aaatattctc ttcatgatgg taattaaggt
     2701 ttaccgtacc gcttttacca tcaacgcgac tggtacctgt aatgttggcc tgcatggtta
     2761 ataaaccgag attatctaaa ttaatatttg tcgatgaacg gctaatttcc atatagcgca
     2821 accagttaat tgctgaaccc gcagtcatat tgtctttcac caccgcatct gcggtgtctt
     2881 tatccagccg caaggtcatt ggcccgctat ttgccagcca accatctttc actatccatt
     2941 tttcgttgtt tagccataac ggcaatgcac cactaaatgc cccggacatg gcgaattgtt
     3001 tcggattgac ggcgctaacc agttcgctgg atgagagatt attcagccgc aacagcgccg
     3061 ggtcatgttg cggcatacgt aattgtttca tcagtacgtt accgcctaac acatcgacgc
     3121 taacatcagt caacagcaag ggttcttctt cagtccacgg ataacgccct tgcaaatcag
     3181 ccgtaatatt tttcgctgtc accagattaa tcacttcggc aattcgcaac gtaacggggc
     3241 cgcgagtccc cagatgccag gctccatcgg caaaacggaa aggcaggaca aaatcgacac
     3301 cgttaacctg attatctggc atccaggcac taccgccttt caacacgccg tgtcctcccg
     3361 cgcggaatcc ttgttcaggc gcagcagaaa atgcaacctg agcatacagt tcaccatcgc
     3421 gtaagttcat cttccagtcg ggtggcacca gcggctggaa tacagtcagt gattgttttg
     3481 gccaccaggc gttaccgcgc agacgaatac cgtcccagcg accatttacc cgaaccgggc
     3541 caatctcacc agcatgtaaa tcgcctttaa agaggaaata cgtcggatcg cgcccatcga
     3601 cgctaaattt taaggttgat ggcggtaaca cactgccgcc agtgaacagc gtttgcccgg
     3661 cgtccagtga cagcgcgccg ctgaaggagg gatgctgtgc gtcacgtacc cagacgacgg
     3721 gcttgtcgag aattaatcgc ggcttttcta ccgtcatcgt accgtattgt aactggtcga
     3781 aaccggtgga gaggtcggtc agcgtaatcg tgctgtcatg ccattcacct tttcctgcga
     3841 catcccaggt ggcattcatc ggtgtaaaac tgccttttcc ccagtagcgc cactgccagc
     3901 ggcccgcgtc agggagaaaa tcattcgcca gcccatccat atgcagcacg aaatcgccca
     3961 gttcattttc atgcgcctgc aagatggcct gcaaacgtcc gtcaacacca cgttgggtga
     4021 cttttacacc cgctaaaggc cagcggattt catcgatgtc cagcgaatcg atgactcttc
     4081 ccttcgaacg taataacgcg cctggctcaa aggccaacgt tgggtcagtc agacttccgc
     4141 ttagctgcgc aggtaaacgg gcatataaaa tgaggtccgc ctgtttcgct tcaccggtca
     4201 gttgcagagg cagctgactg ttatccatgc ttaattttcc tgggccaaaa ttaagcaccg
     4261 cgttgccttt acccgcttgc ccctgggtca gcacactcag tcgtccgctg accagagcgt
     4321 tctcgagccc tgcctgccag ttgtcgactt tgacacccag tcggccactc aaaggaaaac
     4381 ctgcatacgg ccagctccag cgaccatcgc ttacggtcaa ttgttgacga gtaatttgcc
     4441 acggcaaatc gagcaacgga tcgccgttat cccgtgccag cacgatcaat tgcccgctat
     4501 tttcctgcca gtccagctcg gcatccacca gtgaaggctc ctgcggcaag tttaacgtcg
     4561 cagtagcatg accacttaca ggaagtccat ccggcacgag cggcatagta aattccccca
     4621 ccagtttaac cggcgactga ttttcaaacg cgacgacatc cagttcgctg actgtaagtt
     4681 gttgcccttt cagctggcct tgaaatttaa ctttttcgcc ctgataacgc agttgctgga
     4741 tatcagaggt taatgcgaga gagagttttc cctgccattc ctgccattcc tgccagggag
     4801 aaaaaatcag tttatcgata ttgatccagg tgttaggcag catggactgc cactgcgcga
     4861 gggtttttgg agcagctggc gattgctccg tctgcggcaa tttcgccaga caagcagaat
     4921 caagttctac cgtgccgacg ttcaataacc agcggctggg atgtgaaagg ctggcgttgg
     4981 tgatatgcgc aagctgacaa tctcccacca gataacggag atcgggaatg attaaacctt
     5041 tacgcgtaat gcgtgggctt tcatctaatg caatacgtgt cccgagcggt agccagatgc
     5101 ccgccagcgt gggaacccac agcccgagcg tcatcagcag cgtcaacggc acaagaataa
     5161 tcagtaataa cagcgcgaga acggctttat atttacccag catgggtagt taatatcctg
     5221 atttagcgaa aaattaagca ttcaatacgg gtattgtggc atgtttaacc gttcagttga
     5281 aggttgcgcc tacactaagc atagttgttg atgaattttt caatatcgcc atagctttca
     5341 attaaatttg aaattttgta aaatattttt agtagcttaa atgtgattca acatcactgg
     5401 agaaagtctt atgaaactcg ccgtttatag cacaaaacag tacgacaaga agtacctgca
     5461 acaggtgaac gagtcctttg gctttgagct ggaatttttt gactttctgc tgacggaaaa
     5521 aaccgctaaa actgccaatg gctgcgaagc ggtatgtatt ttcgtaaacg atgacggcag
     5581 ccgcccggtg ctggaagagt tgaaaaagca cggcgttaaa tatatcgccc tgcgctgtgc
     5641 cggtttcaat aacgtcgacc ttgacgcggc aaaagaactg gggctgaaag tagtccgtgt
     5701 tccagcctat gatccagaag ccgttgctga acacgccatc ggtatgatga tgacgctgaa
     5761 ccgccgtatt caccgcgcgt atcagcgtac ccgtgacgct aacttctctc tggaaggtct
     5821 gaccggcttt actatgtatg gcaaaacggc aggcgttatc ggtaccggta aaatcggtgt
     5881 ggcgatgctg cgcattctga aaggttttgg tatgcgtctg ctggcgttcg atccgtatcc
     5941 aagtgcagcg gcgctggaac tcggtgtgga gtatgttgat ctgccaaccc tgttctctga
     6001 atcagacgtt atctctctgc actgcccgct gacaccggaa aactaccatc ttttgaacga
     6061 agccgccttc gatcagatga aaaatggcgt gatgatcgtc aataccagtc gcggtgcatt
     6121 gattgattct caggcggcaa ttgaagcgtt gaaaaatcag aaaattggtt cgttgggtat
     6181 ggacgtgtat gagaacgaac gcgatctgtt ctttgaagat aaatccaacg acgtgatcca
     6241 ggatgacgta ttccgtcgcc tgtctgcctg ccacaacgtg ctgtttaccg ggcaccaggc
     6301 attcctgaca gcagaagctc tgaccagtat ttctcagact acgctgcaaa acttaagcaa
     6361 tctggaaaaa ggcgaaacct gcccgaacga actggtttaa tcttgccgct cccctgcatt
     6421 ccaggggagc ttattcagat aatccccaaa gacctttcat cttctattct taaaatagcc
     6481 ctgagtcaga aactgtaatt gagaaccaca atgaagaaag tagccgcgtt tgttgcgcta
     6541 agcctactga tggcgggatg tgtaagtaat gacaaaattg ctgttacgcc agaacagcta
     6601 cagcatcatc gctttgtgct ggaaagcgta aacggtaagc ccgtgaccag cgataaaaat
     6661 cagccagaaa tcagctttgg tgaaaaaatg atgatttccg gcagcatgtg taaccgcttt
     6721 agcggtgaag gcaaactgtc taatggtgaa ctgacagcca aagggctggc aatgacccgt
     6781 atgatgtgcg ctaacccgca gcttaatgaa ctcgataaca ccattagcga aatgctgaaa
     6841 gaaggtgcac aagtggatct gaccgcgaac cagttaacgc tggcgaccgc gaaacagaca
     6901 ttaacttata agctggcgga tttaatgaat taatagctgc cacagctccc ggcggcaagt
     6961 gactgttcgc tacagcgttt gccgttgggt aatgcacaca tcccaatcgc cgtaccatcc
     7021 agttgacggg caacagaaag cgaaccgccg atcattgcac aatttgcttc tccactactg
     7081 gacatcgacg cttttaaacc tggcgctacg tgcgccgcag tcgcctgctg aacaggttca
     7141 ctactacacg ccgacaacaa taaagcggca caccctaccc aaaacgctgc tcgcatctct
     7201 tcttcctctg atcttcaagc caaacgacac cgccataaat aataggcagc acagagggcg
     7261 gcgtcgagag ctgtcctgcg cgttgccccg catttttact tttttatggc tatttttttg
     7321 ccctctgttt gatcaaaaca ttcattacgc tgatgtgggg gacacaaaag cgaaaatgca
     7381 gaagaaagcc atttgctaaa attgaaggat tactactggg cgcgcagcaa tttcgtgcgc
     7441 ccctcatttg cgcaatgtaa gggtgtcata tgattactat tgacggtaat ggcgcggttg
     7501 cttcggtggc gtttcgcacc agtgaagtta tcgccatcta ccctattacc cccagttcca
     7561 cgatggcaga acaggctgat gcctgggccg gaaacggctt gaagaacgtt tggggagaca
     7621 caccacgcgt ggttgaaatg cagtcggaag cgggtgctat cgctaccgtg catggcgctt
     7681 tgcagacggg tgccctttca acatcgttta cgtcatcgca gggtttgctg ctgatgatcc
     7741 cgacgctgta caaactggca ggcgaactca caccgtttgt cctgcatgta gcggcacgta
     7801 ccgttgccac acatgcactc tctatttttg gcgatcattc cgacgtcatg gcggtgcgcc
     7861 agacgggttg cgcgatgttg tgtgcggcaa acgtccagga agcgcaagac tttgctctca
     7921 tttcgcacat cgcgacgctg aaaagccgcg tgccatttat tcatttcttt gatggtttcc
     7981 gcacgtccca cgaaatcaac aaaattgtcc cgctggccga tgacacgatt cttgatctca
     8041 tgccgcaggc cgaaattgat gctcatcgcg cccgggcact caacccggaa catccggtga
     8101 tccgcggtac gtccgccaat cctgacactt atttccagtc tcgcgaagcc accaacccat
     8161 ggtacgacgc ggtctatgac catgttgaac aggcgatgaa tgatttctct gccgcgacag
     8221 gtcgtcagta tcagccgttt gaatattacg ggcatccgca agcggaacgg gtgattatcc
     8281 tgatgggctc tgccattggc acctgtgaag aagtggttga tgaattgcta atccgtggcg
     8341 aaaaagtcgg cgtgctgaaa gttcgcctgt accgcccctt ctccgccaaa cacttgctgc
     8401 aagctctgcc gggatccgta cgcagcgtgg cggtactgga cagaaccaaa gaacccggtg
     8461 cccaggcaga accgctctat ctggatgtga tgaccgcact ggcagaagcc tttaataatg
     8521 gtgagcgcga aaccctgccc cgtgtcattg gtgggcgcta tggtctttca tccaaagaat
     8581 ttggcccgga ctgtgtactg gcggtatttg ccgagctcaa cgcggctaaa ccgaaagcgc
     8641 gctttacggt tggtatttac gatgatgtga ctaatctgtc actgccgttg ccggaaaaca
     8701 ccctgccaaa ctcggcgaaa ctggaagcct tgttttatgg ccttggtagt gatggcagcg
     8761 tttccgcgac caaaaacaat atcaagatta tcggtaattc cacgccgtgg tacgcacagg
     8821 gctattttgt ttacgactcc aaaaaagcgg gcggcctgac ggtttctcac cttcgcgtga
     8881 gcgaacagcc gattcgttcc gcttatctca tttcccaggc tgattttgtt ggctgccacc
     8941 agttgcagtt tatcgataaa tatcagatgg ctgagcgttt aaaacctggc ggcattttcc
     9001 tgctcaacac accgtacagc gcagatgaag tgtggtcgcg cttgccgcaa gaagttcagg
     9061 ccgtgttaaa ccagaaaaaa gcgcgcttct atgtgattaa cgcggcgaaa atcgcccgcg
     9121 aatgtggcct ggcggcccgt attaataccg tcatgcagat ggcttttttc catctgacgc
     9181 aaattctgcc tggcgatagc gccctcgcag aattgcaggg tgcgattgcc aaaagttaca
     9241 gtagcaaagg ccaggatctg gtggaacgca attggcaggc tctggcgctg gcgcgtgaat
     9301 ccgtagaaga agttccgttg caaccggtaa atccgcacag cgccaatcga ccgccagtgg
     9361 tttccgatgc cgctcctgat ttcgtgaaaa ccgtaaccgc tgcgatgctc gccgggcttg
     9421 gtgacgccct ccccgtttcg gcgctgccgc cagacggctc ctggccgatg ggcactacgc
     9481 gctgggaaaa acgtaatatt gccgaagaga tccccatctg gaaagaggaa ctctgtaccc
     9541 aatgtaacca ctgcgttgcc gcttgcccac actcagctat tcgcgcaaaa gtggtgccgc
     9601 ctgaagcgat ggaaaacgcc cctgccagcc tgcattcgct ggatgtgaaa tcgcgtgata
     9661 tgcgcgggca gaaatatgtc ttgcaggtgg ctccggaaga ttgcaccggt tgtaacctgt
     9721 gcgtcgaagt ttgcccggcg aaagaccgtc agaatccaga gattaaagcc atcaatatga
     9781 tgtcgcgcct ggaacatgtc gaagaagaga aaatcaatta cgatttcttc ctcaacctgc
     9841 cagaaatcga ccgtagcaaa ctggaacgta ttgatattcg tacatcgcag ctgattacac
     9901 cgctgtttga atattcaggt gcttgctccg gttgtggcga gacgccgtat attaaattac
     9961 tgactcagct ctatggcgac cggatgttga tcgctaacgc caccggctgt tcttcaattt
    10021 atggcggtaa cctgccctct acaccgtata ccaccgatgc caacggtcgt gggccggcat
    10081 gggcgaactc tctatttgaa gataatgccg aatttggcct tggtttccgc ctgacggtcg
    10141 atcaacaccg tgtccgcgtg ctgcgtctgc tggatcaatt tgccgataaa atcccggcgg
    10201 aattactgac ggcgttgaaa tcagacgcta cgccagaggt tcgtcgagaa caggtcgcag
    10261 ctttacgcca gcaactcaac gatgttgccg aagcacatga actgctacgt gatgcagatg
    10321 cactggtgga aaaatcaatc tggctgattg gtggtgatgg ctgggcttac gatatcggct
    10381 ttggcggtct ggatcatgta ttgagtttga cggaaaacgt caacattctg gtgctggata
    10441 cgcaatgcta ttccaacacc ggtggtcagg catcgaaagc gacaccgctg ggtgcagtaa
    10501 ctaaatttgg cgagcacggc aaacgtaaag cgcgtaaaga tcttggcgtc agtatgatga
    10561 tgtacggtca tgtttatgtg gcgcagattt ctctcggcgc gcagctgaac cagacggtga
    10621 aagcgattca ggaagcggaa gcgtatccgg ggccatcgct gatcattgct tatagcccgt
    10681 gtgaagagca tggttacgat ctggcactca gccacgacca gatgcgccaa ctcacagcta
    10741 ccggcttctg gccgctatat cgcttcgatc cgcgtcgtgc cgatgaaggc aaactgccgc
    10801 tggcgctgga ttcacgcccg ccgtcagtag cactggaaga aacgttactt cacgagcaac
    10861 gtttccgtcg gctgaattcg cagcagccag aagtggcaga acagttatgg aaagatgctg
    10921 cagctgattt gcaaaaacgc tatgacttcc tggcacaaat ggccggaaaa gcggaaaaaa
    10981 gcaacaccga ttaaatgctc tggataagga ttatccaatt ctaaaaaaaa aagcccggac
    11041 gactgttcgg gcttgtcttt ttatatgttg gaaaatcagt ggcaatgcaa tggcccaaca
    11101 gaaaataaaa tctgtgacaa taaaggcata taacccgcgc agaataacgt atagaaaatt
    11161 aaaagtataa tttttatttt gtatgtatta tcttcgatac attcaccaga cttattatca
    11221 tttcagagga attatttgat taaggtttta cttaaggcgt aacaaatgat atttatcggc
    11281 taactgaact tctcctttag gatgttttca ctccccggaa tggggataaa aggcaaataa
    11341 aataactaaa ggatttattc aatgaaaagc aaagtactgg cacttttaat tcctgccctg
    11401 ctcgccgcag gtgctgcaca tgcagccgaa gtttataata aagacggcaa caaattagat
    11461 ctgtatggca aagttgatgg cctgcattat ttttctgata attcagcgaa agatggcgat
    11521 cagagatatg cgcgtctggg ttttaaaggc gaaacccaaa ttaacgatca actcactggc
    11581 tacggtcaat gggaatacaa tattcaggca aacaacactg aatcttcaaa aaaccagtca
    11641 tggacccgtc tggcatttgc cgggctgaaa tttgcagatt acggttcttt cgattacgga
    11701 cgtaattatg gcgtaatgta cgacatcgaa ggctggaccg atatgctgcc tgaatttggc
    11761 ggtgactctt ataccaatgc agacaacttt atgactggtc gagccaatgg cgtcgcgact
    11821 tatcgtaata ctgatttctt cggtctggta aatggtctga acttcgcggt gcagtatcaa
    11881 ggtaacaacg aaggtgccag taatggtcag gaaggcacca acaacggacg tgatgttcgc
    11941 catgaaaacg gtgacggctg gggtctttcc acaacatatg atttaggcat gggctttagc
    12001 gctggtgcgg catacacctc ttctgaccgc accaatgatc aggttaacca tactgcggcg
    12061 ggtggtgata aagcagacgc gtggactgct gggctaaaat acgatgctaa caatatttac
    12121 ctggcaacca tgtattcaga aacgcgtaat atgaccccgt ttggcgacag cgattatgct
    12181 gttgcaaaca aaacccagaa ctttgaagtc actgcacagt accagtttga ttttggtctg
    12241 cgtccggcag tctctttcct gatgtctaaa ggccgtgacc tgcacgctgc cggtggtgca
    12301 gacaacccgg caggtgttga tgataaagat ctggttaaat acgccgatgt tggcgcgact
    12361 tactatttca ataaaaacat gtccacttat gttgactata aaatcaacct gttggatgaa
    12421 gatgacagtt tctacgctgc caatggcatc tctaccgatg atattgtcgc tttaggtctg
    12481 gtttatcagt tctaaatcct cctgcccgct gttatggcgg gctttttctg cttattcttc
    12541 ctcctttgat ctaaattaaa aatgtgaact ccgtcattac acaaaaagtg tcatctggcg
    12601 ttacacttta tgcggatact aaaacaggag gttttatgaa cagaacgatt cttgtcccta
    12661 tcgatatttc cgattcagaa ttaactcaac gcgtgattag ccacgttgag gcagaggcaa
    12721 agattgatga tgcagaggtt catttcctga cggtaatacc ttcactgccc tactatgcct
    12781 ctctgggttt agcgtattcc gcagaattac cggcaatgga tgacctgaaa gcggaagcca
    12841 aatcgcaact ggaagagatc attaaaaaat ttaaactgcc aaccgacaga gtgcatgtcc
    12901 atgttgagga aggctcgccc aaagaccgca ttctggaaat ggcgaagaag atccccgctc
    12961 atatgatcat cattgcttcc catcgaccgg atatcaccac ttatctgctc ggttccaacg
    13021 ccgcagctgt agtgcgtcac gcagagtgct ccgtgctggt tgtgcgctga cactaacgcc
    13081 cgcacattgc tgcgggcttt ttgattcatt tcgcaaatgt gctgacattt tcccctctaa
    13141 tccgtaccat acacgccaca gtttttatat cagatttctt atgctgggcg ttccggcatg
    13201 acgcatactc ttctgatgcc atataacgaa ttgagtcgct tttaaatgtc gcaaaatcaa
    13261 gaaattagta agaaagaaca atacaacctg aacaaattac aaaaacgtct gcgtcgtaac
    13321 gtgggcgaag ccattgctga cttcaatatg attgaagaag gcgatcgcat catggtttgc
    13381 ctctccgggg gtaaagacag ctataccatg ctggagattc tgcgcaattt gcagcaaagc
    13441 gcgccaatca atttttcgct ggtggctgtt aacctcgatc aaaagcaacc gggcttcccg
    13501 gaacacgttc tgcccgagta tcttgaaaag ctgggcgttg agtacaagat tgttgaagag
    13561 aatacttacg gtatcgtgaa agagaagatt ccagagggca aaaccacttg ctcactgtgt
    13621 tctcgccttc gtcgcggtat cctttatcgt accgcaacgg aactgggggc gacgaagatc
    13681 gcgttgggtc accatcgtga cgatatcctg caaacgttgt tcttaaatat gttctacggc
    13741 ggcaagatga aaggtatgcc tccgaaactg atgagcgatg atggcaaaca tatcgttatt
    13801 cgtccgctgg cctactgccg cgagaaagat attcagcgat ttgccgatgc aaaagcgttc
    13861 ccgattattc cgtgcaacct gtgcggttca cagcctaacc tgcaacgtca ggtgattgct
    13921 gacatgttgc gtgactggga taaacgttat ccagggcgta tcgagacgat gttcagcgcg
    13981 atgcagaatg tggtgccgtc gcatctgtgc gataccaacc tgttcgattt caaaggcatt
    14041 acccacggtt ctgaagtggt taacgggggt gatctggcgt ttgatcgcga agagatccca
    14101 ctacaaccgg cgggctggca gccagaagaa gatgaaaatc agttggatga gttacggctg
    14161 aatgtggttg aagtgaaata accaggatag cgcccgatgc gcaagcgtat cgggctactc
    14221 ttatggaggc cggataagac gcggccagcg tcgcatccgg caatcccgca taagatgttt
    14281 actcttgcac ccggcaattc aacatttcat tattttaata accgcacccg gcacgttttt
    14341 cctttaatct tcccgccctg taactgtttc catgctttat gagcaacagc ctgacggacc
    14401 gcgacataga catgcgccgg atgcacggcg attttgccaa tatctacgcc atcaagcccg
    14461 atgtctcctg tcagcgcacc taatacatca cccgggcgca ttttggcttt tttcccgcca
    14521 tcgatacaca acgttgccat ttctgcttcc agcggcaaaa tggaactatt agctggcggc
    14581 gtttgccagt taagttttat ctgcaacatg tcagaaatga tattggcccg ctgtgcttct
    14641 tccggagcac agaaactgat cgccagaccg ctatttcctg cacgagctgt acgaccgatg
    14701 cgatgtacat gaacttcagg gtcccacgcc agctcaaagt tcaccaccag ctcaagcgat
    14761 ttaatatcca gaccacgcgc agcaacatca gtcgcaacca gtacacgggc gctaccgtta
    14821 gcaaaacgta ccagagtctg atcgcgatcg cgttgctcca gatcgccgtg taacgacaat
    14881 gcactttgcc ctacttcatt cagcgcgtcg cagacagcct ggcaatcttt tttggtattg
    14941 caaaacacca cacaagagga tggctgatgc aagcttaata accgttgcaa cagaggaatt
    15001 ttgcctttac tggatgtctc ataaaattgt tgttcaatag gtggcaaagc atctgttgag
    15061 tcaatttcaa tcgccaaagg atcgcgttgc actcgtccgc tgattgcggc gatggcttcc
    15121 ggccaggttg ccgaaaacag aagcgtctgt cgagatgcag gcgcaaaacg gatgacatca
    15181 tcaatggcat cgctaaatcc catatccagc atgcggtcgg cctcatccat caccaaagta
    15241 ttcagcgcat ccagtgatac cgtgcctttt tgcaagtgat ccagcaaacg acccagcgtt
    15301 gccacgataa tatgcggcgc atgttgcaac gaatcacgct gcataccgaa cggttgacca
    15361 ccgcacaacg tcaaaatttt ggtattcggc agaaaacgcg ccagccgacg caattcacct
    15421 gccacctgat ccgccagttc acgcgtagga cacagcacta aagcctgggt ttgaaatagc
    15481 gacgcatcaa tttgctgtaa caagccgagg ccaaaagccg ccgttttgcc gctgccggtt
    15541 ttcgcctgca cgcgaacatc ttttccggca aggatcgccg gaagcgcggc ggcctgcacc
    15601 ggcgtcatgg ttaaataacc caactcatta aggttcgtga gttgggcggg aggcaaaaca
    15661 ttcagggtag aaaaagcggt cacaatctat tctcgtggtc atcgacgcaa agttagcagg
    15721 cgcgtatcct cgcagatcta cgctcacgat gcgacaattt aatcggttct tcatcaggtg
    15781 gtgggtcagg catgggttgc gggcgaggga tcggatcggg cactggaaca ggatcgccag
    15841 gaatcggttc agggacagga atttgcaaat aaataagtgt cgtcatattt ccctccggtc
    15901 attgggtgga ctcttaaagg gtagacgctg ataaataaca ggcaaaaaaa agccgactca
    15961 tcaaagtcgg cgtcgtacga atcaattgtg ctatgcagta attcaaaaaa ggaagtaaga
    16021 caatatggag cgcaacgccc atcgcttgac gttgcattca cctgcaaggg agatattgcc
    16081 ctgaatgggt agagagttta ttgacttcgc tcaaactttg cggcgttttt gtatacagac
    16141 agccggaaaa attgcttttg ttacaaccat ttactacgat gcaaccataa agcaacacca
    16201 ccaataagaa caactaacag aatacaaaaa attgaaaatc cgaattgcca cccgccgcca
    16261 gggatcccac caaggttgac gccaaataac cctgtcagaa aggtactggg taaaaagacc
    16321 attgccatca atgacattgt ataggtacga cgagctaaat tttcctgcat cacctgagcg
    16381 atttcatccg ccatcacgcc agtccgtgct atacaggcgt cgatttcgtc aagaccgcgc
    16441 ccaaggcgat cggcaatatc ctgcatccga cggcgttggt catcgctcat ccacggcaaa
    16501 cgttcactgg caagacgagc ataaacatca cgttgcggtg ccatatagcg acgcatcacg
    16561 attaattgtt tgcgcagcag agccaggaat ccacgcggtg gaatttgctg atcaaggaga
    16621 ttatcttcaa ggtcgataat tttatcgtgc agctgctcga taaattcgct ggaatgatcg
    16681 gtcaacgcat cgcacacatc caccagccac cctccgcaat cggtcggacc cgtgccctct
    16741 tccagatcgc tcaccacatc gtccagcgcc agcactttgc gttgtcgggt cgaaacaatt
    16801 aaccgcccgt ccatatatac acgcatggcg accagttgat cggggcgttc atcggtgctg
    16861 ccgtttatac agcgcaatgt aatcagcgtg ccttcaccga gacggctgac tcggggccga
    16921 gtgctctcgc ctgccagcgc atcacgtacg ttattgggaa gcagcggtgt tgtcgccagc
    16981 cattgggcgc tttcatggtg tacataatta aggtggagcc agcagggatg cgcttcatca
    17041 atcacatctg tattttccag cggtttaacg ccgcctctac catccagcat ccaggcaaat
    17101 actgcatccg ggacattaac gtccgatccc ttaatcgctt ccacagtgcc tccatcatca
    17161 acgcattatt ttgtagtcta gccttctggc cctgttacgc aacatctcat caccccatta
    17221 ccctgaaatg attaataaaa ttctgtctaa attgaataca aaaagcaaaa tgcttttccg
    17281 tatacaaacc gtgtgaagtg ttaaatagcg tctatcatta tcagaattat ctgatcatat
    17341 gacgtggctt ttttgcgatc ggatagcaac aaaaattgat aaaaataacg ggatctcaat
    17401 gattacgcac aacttcaata ccctggactt actcaccagt cctgtctgga ttgtttcgcc
    17461 ctttgaggaa cagttaattt atgccaatag cgcggcgcga ctgttgatgc aagacctcac
    17521 gtttagtcag ctacgaaccg gaccctattc cgtctcctca caaaaagaac tgccgaaata
    17581 cctctccgat ctgcaaaacc aacacgatat tatcgaaatc ctcactgttc agcgtaaaga
    17641 agaggaaaca gcattaagct gtcggcttgt tttgcgagag ctgacagaaa cagaaccggt
    17701 gattattttc gaaggtatcg aagcgccggc aacgctgggt ttaaaagcca gtcgctcggc
    17761 aaattatcag cgcaaaaaac aaggttttta tgcgcgcttt tttctgacta actctgcacc
    17821 aatgttgttg attgacccct cacgagatgg ccaaatcgtc gatgctaacc tcgccgcgct
    17881 caatttctat ggttataacc atgaaacgat gtgccagaaa catacctggg aaattaatat
    17941 gctcgggcgt cgcgtcatgc ctatcatgca tgaaatttcg catttacccg gcggtcataa
    18001 acctttgaat tttgttcata aactggcgga tggatctact cgtcatgtgc agacctatgc
    18061 cgggccgatt gaaatttatg gcgacaagct catgttatgt attgtgcatg atattactga
    18121 gcaaaaacgg ctggaggagc agctggaaca tgctgctcac catgacgcga tgaccggatt
    18181 actgaatcgg cgacagtttt atcacattac cgagccaggc caaatgcagc atctcgccat
    18241 cgctcaggat tacagcttgt tgctcatcga caccgatcgt tttaaacaca ttaacgatct
    18301 ctatgggcat tctaaaggtg atgaggtgtt atgcgccctc gcccgcaccc ttgaaagttg
    18361 cgctcgcaaa ggcgatttgg tgtttcgttg gggaggcgaa gagtttgtct tattgctacc
    18421 aagaacccca ctggataccg cgctttcgct ggctgaaact atccgtgtaa gcgtggcaaa
    18481 agtgagtatt tcgggcttac cacgctttac cgtcagcatt ggtgtggcgc atcacgaagg
    18541 aaatgaaagc atcgatgaac tgtttaaacg cgttgatgat gctttgtatc gggcgaaaaa
    18601 tgatggacgc aaccgcgtgc tggcggcata agccgcggat gcgtctcgag atcaacgact
    18661 gcgcttagcg tggcgctccc agttttcttg cttcgcctgc gccgttttac gtagtgcgac
    18721 gtaacacgcc ccgctgccgc catgatgcgg tagcgcagtg caatatgcct gaacatcatc
    18781 aaattcggtc agccagcgcg ccacatagct gcggacaata ttggcatgcg atttatcatc
    18841 ccgtcctttg ccatgaataa tcagcacgtt acgcaaacca tcagccatgg cttgttgaat
    18901 gaaactgaac atcattttgc ggcactcttc caccggctgg cgcaaaagat tcaggctcgc
    18961 ctgttgcgga tatttaccac tgcgcagctt atccagcacc ccatgttgca acccttcccg
    19021 ccgaaactcc agcggctgac ttagtgggat gatgtcgaga aatccggtgg tgaggaaatt
    19081 atcaagctgc agcgtgtcga tacgctgcgg cgcacgttgg ttacgcgttg gatgccagtg
    19141 gacatcggta gcacgtttca gcggctggac atcttccatg gcgtcaagaa acagcgattt
    19201 gtcgtcaagg ttcatgttac atcctcccgc aattaagagc gcgatatgat aaccagaccg
    19261 ggtcggtcca acaacgtatt acccaaaatt ccagtaataa gttccaaata ttgccgatat
    19321 tttaagcaaa atgcttatgc atgattattc attcacgata ttaataatgt aacttatatt
    19381 ttcgtgaaat ctgtcactga agaaaattgg caactaaagg ttaaaaccgt tataacacag
    19441 tcaccggcgc agaggagaca atgccggact taagacgcgg atgcactgct gtgtgtactg
    19501 tagagtctgg cggatgtcga cagactctat ttttttatgc agttttaact ttgcagatag
    19561 ccgcattctc gccggaattg cgtgattaat gacgcggtca gtggtgtctg gcgactatca
    19621 cgccgctgaa tcaaataata ggccgctttc ggtaaaattt cgctaaccgg caacatcacc
    19681 agcccctgtc cgtgcaaggg atcgcagccc atttcttcag gcagtatgct gagaaaatcg
    19741 ctttttgcca ccagactgat acaggctgag aacgtctcgc agactacacc gacctgtggc
    19801 gtttgcgcct gatcgtcaag caattcactc aactgtttgt agtaactgcc gtgtggcgtc
    19861 ggcattgtcc agctgtaatc cagtaactgt ttgatcgaac gggcaccaat ggcggggtgt
    19921 cccgggcggc aaaagatcgc gaattgcttt tccagtaatt tctcaaaagt aaattcgtgg
    19981 tcgtacggtc cctgataata ggtattgatg gtgaaatcca attctccctg acgcaattca
    20041 ttaatcatcg acaccagttg cccttccata atgcgtactt ttacctgcgg atgctgctga
    20101 tgaaaacgag atatgacagc tggcatcaga ctgcgggaaa tactggcccc catgccgata
    20161 ttaatctgcc ctgccagttg cccttgtcgt tggcgaattt cctcttgcgc tgcgcgcagc
    20221 tcttcaagaa taagactggc gtgctgataa aaactttcac cggcatcagt taacgtcacg
    20281 cctttactac ggcgaaaaaa gagttgcgcc gctaatcctt cttccagctc ctgaatagat
    20341 ttactcagtg ccggttgcga catattcaac attcggctcg ctccgcgaat gctgccctga
    20401 cgagccactt caacaaaagc acgaatttga tgaattttta cctgaaaagc catgacgcca
    20461 ccgataaccg ttatttatca gaccaaagaa actggcatct actttaatgc agatgattgt
    20521 gtcagggtaa tttatgaacg gttaaaactg tgaaaaatca gttagtgata agtaaaaact
    20581 atcgctacat gaaccgggtc acacttttta ctgatgacgg gaaaggttat ggagtctttg
    20641 aatcaatttg ttaattcgct tgccccaaaa ttatcgcact ggcgacgtga ttttcatcac
    20701 tatgcagagt ctggctgggt ggaattccgc actgccaccc ttgttgcgga agaattgcag
    20761 cagctcggct attcactggc gctgggccgc gaagtagtta atgaaagtag ccggatggga
    20821 ctacctgatg aactcactct acaacgcgaa ttcgagcgcg ctcgtcaaca gggtgcgcta
    20881 gcacaatgga ttgcggtttt tgaaggtggt ttcactggta tcgtcgccac cctggatacc
    20941 ggtcgccccg gtccggtgat ggctttccgt gtcgatatgg acgcgctgga tctcagtgaa
    21001 gagcaggatg tcagccatcg cccctaccgc gacggttttg cgtcatgtaa cgccggaatg
    21061 atgcatgcct gtggtcatga tggacatacc gccattgggc ttgggctggc gcataccctt
    21121 aaacaattcg agtccggact acatggcgtc atcaaactga tttttcagcc tgcagaggaa
    21181 ggtacgcgtg gcgcgcgggc gatggtcgat gcaggtgtcg tagatgatgt tgattatttt
    21241 actgccgtgc acattggcac tggcgtacct gcgggcaccg tggtgtgcgg cagtgataat
    21301 tttatggcaa ccaccaaatt tgacgcgcac ttcaccggga ccgccgctca cgcaggcgca
    21361 aaaccagaag acggtcacaa tgccttgttg gcggcagcac aagcaactct tgcactgcat
    21421 gcaatcgccc cgcacagcga aggagcttcc agagtaaacg tgggcgttat gcaggcagga
    21481 agcggccgta acgttgttcc tgcctcggcg ttgctgaaag tggaaacacg cggggccagc
    21541 gacgtcatta atcaatatgt ttttgaccgt acacaacaag cgattcaggg cgcagcaacc
    21601 atgtatggtg tcggcgttga aactcgtctg atgggtgcag ctaccgccag ttctccttcg
    21661 ccgcaatggg tcgcatggtt gcaaagtcag gcggctcagg tcgcgggggt caatcaggcc
    21721 attgaacgtg ttgaagcgcc tgcgggttcc gaagatgcca cattaatgat ggcccgcgtg
    21781 cagcaacatc aagggcaagc ctcctacgtg gtgtttggca cacagctggc ggcaggtcat
    21841 cacaacgaaa aattcgattt tgacgagcag gttctcgcta ttgccgtcga aacgctggcg
    21901 cgcaccgcgc tcaattttcc ctggacgcga ggtatctgat gcaggaaatc tatcgtttta
    21961 tcgacgatgc gattgaagcc gatcgccaac gttataccga tattgccgat caaatctggg
    22021 atcatccaga aacacgtttt gaagagttct ggtcagcgga gcatctggct tcggcgctgg
    22081 aatctgcaag cttcaccgtt acccgcaacg taggcaatat cccaaatgca tttattgctt
    22141 cgtttggtca aggcaaaccg gttatcgccc tgctgggaga atatgacgcc ctggcaggtt
    22201 taagtcagca agctggttgc gcgcaaccta catccgcgac gcccggtgaa aatggtcacg
    22261 gttgcggaca caatttgctg ggaaccgccg cctttgccgc tgcaatagcc gtcaagaaat
    22321 ggctggaaca atatgggcaa ggcggcacag tgcgctttta tggttgtcct ggcgaagaag
    22381 gcggctcggg taaaacgttc atggtccgcg agggggtatt tgatgatgtg gatgcggcac
    22441 tcacctggca cccggaagcc tttgccggca tgttcaatac ccgcacgctg gcaaacattc
    22501 aggcatcatg gtgctttaaa gggatcgcag cacatgccgc gaattcccct catttgggac
    22561 gcagcgccct tgatgccgta acgttgatga ccactggcac caacttcctc aacgaacata
    22621 ttattgaaaa agcgcgcgta cactatgcca tcacaaatag cggcgggatc tcgcccaacg
    22681 tggtccaggc gcaggcagaa gtgctttatc ttatccgcgc ccccgaaatg accgacgtgc
    22741 agcatattta tgatcgggtc gccaaaatcg ccgaaggtgc ggcattgatg accgaaacca
    22801 cggttgaatg ccgcttcgac aaagcctgtt ccagttatct cccgaatcgc accttagaaa
    22861 atgccatgta ccatgcccta tcccattttg gtaccccgga atggaactcc gaagaactgg
    22921 cttttgcgaa acaaattcag gctacgctca cccccaacga tcggcaaaac agtctgaata
    22981 atatcgccgc aaccggtggc gaaaacggca aggcttttgc actacgtcat cgtgaaacgg
    23041 tactggcgaa tgaggtcgct ccatatgccg ccaccgataa cgtgcttgcg gcatcgactg
    23101 atgtcggcga cgtcagttgg aaactgcctg ttgcccagtg tttcagcccc tgttttgccg
    23161 tcggtacacc gctacatacg tggcaactgg ttagccaggg gcgaacatct attgctcata
    23221 aagtaatgct gctggcggcg aaaactatgg cagcaaccac agtcaatctc ttcattgatt
    23281 cagggctatt gcaagaatgc caacaagagc atcagcaagt tacggacacg caaccgtatc
    23341 actgccctat cccgaaaaac gtgacaccgt cacctttaaa ataacaacaa caacgcaaac
    23401 acaacaaccg aggaatgccc atgagtatgt catccatacc gtcgtcctcc caatccggga
    23461 agctctatgg ctgggtcgaa agaattggta acaaggttcc ccatcctttt ttgctcttta
    23521 tctatttgat aatcgtactc atggtgacga cggcaatttt gtcagccttt ggcgtcagtg
    23581 cgaaaaaccc gaccgatggt acgccggtgg tggtgaaaaa cctgctcagt gtagaaggat
    23641 tacactggtt tttacccaat gtgattaaaa actttagcgg ttttgctcca cttggagcga
    23701 tcctggcgct ggttttaggt gccggcctag cggagcgcgt cggcttactg ccagcgctaa
    23761 tggttaaaat ggcatcgcat gttaatgccc gctacgccag ttatatggtg ctgtttattg
    23821 cttttttcag ccacatttct tccgatgcgg cgttagtgat catgccaccg atgggtgcgc
    23881 tgatttttct ggcggtgggc aggcatccag ttgcaggttt actggccgcc attgcaggcg
    23941 taggttgcgg cttcacggct aatttactga ttgtcacaac cgacgtgttg ctgtcgggga
    24001 tcagcacgga agcggcggct gcgttcaatc cgcaaatgca cgtcagtgta attgataact
    24061 ggtattttat ggccagctcc gtagtcgtac tgacgattgt tggcggcctg ataaccgaca
    24121 aaatcatcga gccacggtta ggtcaatggc agggaaacag cgatgagaaa ctgcagacat
    24181 tgaccgaaag tcagcgtttt ggtttacgca tagcaggtgt cgtatcgcta ctttttattg
    24241 ctgcgatcgc gctgatggtg atcccggaaa acgggatatt gcgcgatccg attaatcaca
    24301 ccgtgatgcc atcacccttt attaaaggta tcgtgccact gatcattctt tttttctttg
    24361 ttgtctcgct ggcttatggc atcgctaccc gcacaattcg acgtcaggcg gatttaccgc
    24421 atttaatgat tgaaccgatg aaagagatgg cgggatttat cgtgatggtt tttcccctag
    24481 cccagtttgt cgccatgttt aactggagca acatggggaa attcatcgcc gtggggctga
    24541 ccgatatact tgaaagttca gggcttagcg gcatcccggc gtttgtcggt ctggcgttgc
    24601 tttcctcttt cttatgcatg tttattgcca gcggttccgc aatctggtcg attctggccc
    24661 ccattttcgt accaatgttt atgctacttg gctttcaccc ggcatttgcg caaatcctct
    24721 ttcgtattgc cgactcatcc gtattgcctt tagcgccggt atctcctttt gttccactgt
    24781 ttcttggatt cctgcaacgc tacaaaccag acgcgaaact gggtacttac tattcgttag
    24841 tcttgcccta tccgcttatc tttttggtgg tatggctgct gatgttgctg gcgtggtatc
    24901 ttgttggtct gccgataggt ccggggattt acccacgttt gtcttaagag agaacggatg
    24961 ctgagattac ttgaagaaaa aattgccacg ccactgggtc cactgtgggt gatttgcgat
    25021 gagcaatttc gcctgcgggc ggttgaatgg gaagagtaca gcgaacgcat ggtgc
//
LOCUS       DAMZET010000054        25074 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.054, whole genome shotgun sequence.
ACCESSION   DAMZET010000054 DAMZET010000000
VERSION     DAMZET010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.054"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..231)
                     /locus_tag="POB34_002234"
     CDS             complement(<1..231)
                     /locus_tag="POB34_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="part of the GlrKR two-component signal transduction
                     system involved in the regulation of glmY; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDI5919171.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCV"
     gene            complement(789..4676)
                     /gene="purL"
                     /locus_tag="POB34_002235"
                     /gene_synonym="purI"
     CDS             complement(789..4676)
                     /gene="purL"
                     /locus_tag="POB34_002235"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDI5919172.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAIAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALAREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATVARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            4934..6490
                     /gene="mltF"
                     /locus_tag="POB34_002236"
     CDS             4934..6490
                     /gene="mltF"
                     /locus_tag="POB34_002236"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HDI5919173.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(6487..7023)
                     /gene="tadA"
                     /locus_tag="POB34_002237"
     CDS             complement(6487..7023)
                     /gene="tadA"
                     /locus_tag="POB34_002237"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDI5919174.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(7048..7683)
                     /gene="yfhb"
                     /locus_tag="POB34_002238"
     CDS             complement(7048..7683)
                     /gene="yfhb"
                     /locus_tag="POB34_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HDI5919175.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            7892..8740
                     /locus_tag="POB34_002239"
     CDS             7892..8740
                     /locus_tag="POB34_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDI5919176.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            8796..9056
                     /gene="yfhL"
                     /locus_tag="POB34_002240"
     CDS             8796..9056
                     /gene="yfhL"
                     /locus_tag="POB34_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HDI5919177.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(9250..9330)
                     /gene="shoB"
                     /locus_tag="POB34_002241"
     CDS             complement(9250..9330)
                     /gene="shoB"
                     /locus_tag="POB34_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HDI5919178.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(9751..10131)
                     /gene="acpS"
                     /locus_tag="POB34_002242"
     CDS             complement(9751..10131)
                     /gene="acpS"
                     /locus_tag="POB34_002242"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDI5919179.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGDRLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVANMHVTLADERHYACATVIIES"
     gene            complement(10131..10862)
                     /gene="pdxJ"
                     /locus_tag="POB34_002243"
     CDS             complement(10131..10862)
                     /gene="pdxJ"
                     /locus_tag="POB34_002243"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HDI5919180.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(10874..11602)
                     /gene="recO"
                     /locus_tag="POB34_002244"
     CDS             complement(10874..11602)
                     /gene="recO"
                     /locus_tag="POB34_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDI5919181.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(11614..12519)
                     /gene="era"
                     /locus_tag="POB34_002245"
     CDS             complement(11614..12519)
                     /gene="era"
                     /locus_tag="POB34_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDI5919182.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(12516..13196)
                     /gene="rnc"
                     /locus_tag="POB34_002246"
     CDS             complement(12516..13196)
                     /gene="rnc"
                     /locus_tag="POB34_002246"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDI5919183.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(13468..14442)
                     /gene="lepB"
                     /locus_tag="POB34_002247"
     CDS             complement(13468..14442)
                     /gene="lepB"
                     /locus_tag="POB34_002247"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDI5919184.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGLRLSRIGGIH"
     gene            complement(14458..16257)
                     /gene="lepA"
                     /locus_tag="POB34_002248"
     CDS             complement(14458..16257)
                     /gene="lepA"
                     /locus_tag="POB34_002248"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDI5919185.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(16455..16934)
                     /gene="rseC"
                     /locus_tag="POB34_002249"
     CDS             complement(16455..16934)
                     /gene="rseC"
                     /locus_tag="POB34_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HDI5919186.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(16931..17887)
                     /gene="rseB"
                     /locus_tag="POB34_002250"
     CDS             complement(16931..17887)
                     /gene="rseB"
                     /locus_tag="POB34_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HDI5919187.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(17887..18537)
                     /gene="rseA"
                     /locus_tag="POB34_002251"
     CDS             complement(17887..18537)
                     /gene="rseA"
                     /locus_tag="POB34_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HDI5919188.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(18570..19145)
                     /gene="rpoE"
                     /locus_tag="POB34_002252"
     CDS             complement(18570..19145)
                     /gene="rpoE"
                     /locus_tag="POB34_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HDI5919189.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(19142..19297)
                     /gene="rseD"
                     /locus_tag="POB34_002253"
     CDS             complement(19142..19297)
                     /gene="rseD"
                     /locus_tag="POB34_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HDI5919190.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            19553..21175
                     /gene="nadB"
                     /locus_tag="POB34_002254"
     CDS             19553..21175
                     /gene="nadB"
                     /locus_tag="POB34_002254"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HDI5919191.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(21160..21897)
                     /gene="trmN"
                     /locus_tag="POB34_002255"
     CDS             complement(21160..21897)
                     /gene="trmN"
                     /locus_tag="POB34_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HDI5919192.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWVTQQTARFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            22029..23363
                     /gene="srmB"
                     /locus_tag="POB34_002256"
     CDS             22029..23363
                     /gene="srmB"
                     /locus_tag="POB34_002256"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HDI5919193.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(23572..24453)
                     /locus_tag="POB34_002257"
     CDS             complement(23572..24453)
                     /locus_tag="POB34_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI5919194.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            24556..>25074
                     /gene="eamB"
                     /locus_tag="POB34_002258"
     CDS             24556..>25074
                     /gene="eamB"
                     /locus_tag="POB34_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HDI5919195.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQQLFRQ"
BASE COUNT         6079 a         6722 c         6291 g         5982 t
ORIGIN      
        1 cacgcaatac tgacggtagc tacgctccat ctccagcgcc gcgttggtca tcgcttcact
       61 acgccgggca tcaataagcg tagtgcggtt aaccagcgcc gcctgatcgc taagcgcatt
      121 caggctttgc caggcttgcc atgccagcac caacaggggt agtagaatta gcaaaaatgc
      181 cagcattacc agttgtcgta atgagcgggg aaaaacgggc cagcgtttca aggtgttact
      241 ctcgtcagac gcgaatagcc tgatgctaac cgaggggaag ttcagataca acaaagccgg
      301 gaattacccg gctttgttat ggaataaggc ggtgcctaac tcgacgtttc gcccgatggt
      361 tgatatagct acgctgatat cagaagttgg acggcaggca ccttgttgtg cgtcattcga
      421 attttatgta gcacgtcccg aaggggctga cataagtcgg tgaatgagcc actgcttact
      481 attatgcagt aactgtgcca ataaagaaaa tagtttggta acgtactgat tatacgttgc
      541 tttgaggggt ttatgtcttc tccgctgttt gaatgtaaat cagccactgt gtcgctaaaa
      601 agagacaact taagataaac ttattagata atattaaaat caatgagtta agtgtcgcca
      661 gaaagcgaca cggcaaacca cccattgtcg tgatttacag acacaaaaaa gctcccggag
      721 ttgggagctt atgatagtgg ttggtgctta atgccgcatc cgggctgcaa aaccaatggg
      781 ctgacgactt accccaactg cttacgcgca ttgcggaaaa tacgcatcca tgggccatcc
      841 tcgccccagt tttccggatg ccaggagttg ctgacagtac ggaaaacacg ttccgggtgc
      901 ggcatcataa tggtgactcg accactttca gtcgtgactg ccgtaatacc gttcggtgaa
      961 ccgttcgggt tagccgggta ggtttcagtg actttgccga agttatcgac atagcgcagt
     1021 gccaccagcc ctttgctttc cagtgccgcc agatgcgccg catcacgcac ttccacgcgc
     1081 ccttcaccat gagagacagc aatcggcatt tgcgagccca ccatcccctg caacagcagc
     1141 gacgggcttt gggtcacttc aaccaggctg aaacgcgctt caaagcgatc ggaggtattg
     1201 cgcacaaaac gtggccacaa ctcactacct gggatcagtt cgcgcaggtt agacatcatc
     1261 tggcaaccgt tacatacccc cagcgccagc gtttgcggac ggtggaagaa ggttgcaaac
     1321 tcatcgcgta cacggtcatt gaacaggatt gacttcgccc aaccttcacc ggcacccagc
     1381 acatcaccgt aggagaaacc accgcacgcg accagggcgt ggaaatcttc caggcccgtg
     1441 cgtccggtca gcaggtcact catatgcacg tcgatagcat caaagcctgc acggtggaaa
     1501 gctgccgcca tttcaacatg cgagttcacg ccctgctcac gcagtacggc aactttcgga
     1561 cgtgcgacag tggcaatata cggtgctgcc acatcttcgt tgatatcgaa cgacagtttt
     1621 acattcaggc caggatcggc atcgttagat ttcgcctggt gctcctggtc agcacactcc
     1681 gggttgtcac gcaggcgctg catctgccag gtagtttctg cccaccagac acgcaacgtg
     1741 gtgcggcttt cgctgaatac agtctgcccg ttggcggtaa tcacaaaacg gtcaccggaa
     1801 accgcctgcc ctacataatg gacacaatca gcaagcccat gctgtgccag tacggactca
     1861 accgcttcac ggtcagcggc acgaacctga atcaccgcac ccagttcttc gttaaacaac
     1921 gccgccaggc gatcgtcgcc cagagaggca atatccgcgt taatgccgca atgaccagca
     1981 aaggccattt ccgccagcgt taccagcagg ccgccatcag agcggtcgtg gtatgcgagc
     2041 agcttacgct gtgcaaccag cgcctgaatc gcgtcataga agcctttcag ttgcgcgacg
     2101 tcgcgtacat ctgccggttt gtcgccaagc tgacgataaa cctgcgccag cgccgttgcg
     2161 cccagcgcgt tattgccttt gcccaaatca atcagcagca gtgcgttatc ttcggtagaa
     2221 agctgtggcg tgatggtgtg acgcacatct tccacgcggg caaatgcaga aatcaccagc
     2281 gacagcggcg acgtcatttc gcgctcttcg ttaccttcct gccagcgggt tttcatcgac
     2341 atagagtctt tacccaccgg gatcgtcagg cccagcgccg gacaaagctc ttcgcccacg
     2401 gctttaacgg cttcatacag gcccgcatct tcaccagggt ggcctgccgc cgccatccag
     2461 ttggcggaaa gtttaatgcg tttgatatca ccaatctgtg ttgcggcaat attggttaac
     2521 gcttcaccga ccgccagacg ggcagaggcg gcgaaatcca gcagcgcaac cggcgcacgc
     2581 tcgccaatcg ccatcgcttc accgtagtag ctgtcgaggc tggcggtagt gaccgcgcag
     2641 ttagcgaccg gcacctgcca cggccccacc atctgatcgc gcgctaccat gccggttacg
     2701 ctgcggtcgc caatggtcac caggaaggtt ttttccgcca cagtcggcag atgcagcaca
     2761 cgtttcaccg catctgcaat ggtgatccct tcacgggcca gcgcgtcgcc tttcgctttc
     2821 agcgtttgta catcgcgggt catcttcggc gttttaccaa gcaggacgtc cagcggcaga
     2881 tcgatcggct gattatcaaa atgacgatcg tgcagagaaa gatgcagttc ttcggtcgct
     2941 tcaccaatca ccgcgtaggg tgcgcgttca cgcttacaca gttcgtcaaa cagcggtaat
     3001 tgatcggcag caatcgccag cacgtagcgt tcctgggatt cgttacacca gatttccagc
     3061 gggctcatgc ccggttcgtc gcttagaata tcgcgcagtt caaatttacc gccgcgtccg
     3121 ccgtcgctca ccagctccgg catcgcgtta gaaagaccgc ccgcaccgac gtcgtggata
     3181 aacagaattg ggttagcatc accaagctgc cagcaacggt cgatcacttc ctggcaacga
     3241 cgctccatct ccgggttgtc gcgctgtacg gaagcaaagt cgaggtcggc atcagactga
     3301 cctgacgcca tggaagacgc cgctccgccg ccaagaccga tgttcattgc cgggccgccg
     3361 agaacgacca gcttcgcacc gacgttgatc tcgccttttt gtacgtgatc ggcgcgaatg
     3421 ttgccgatcc cgcccgccag catgatcggt ttgtgataac cgcgcagctc ttcgccgttg
     3481 tggctgttca ctttttcttc ataagtacgg aagtagccat tcagtgccgg acgaccaaat
     3541 tcgttgttaa acgccgcgcc gcccagcggg ccttcggtca tgatgtccag cgcggtgaca
     3601 atgcgctcag gcttaccgaa atcttcttcc cacggctgtt cgaagccagg aattcgcagg
     3661 ttggaaacgg agaaaccaac caaacccgct ttcggcttcg cgccgcgccc ggtggcacct
     3721 tcatcgcgga tttcaccgcc ggaaccggtc gccgcgcccg gccacggaga aatcgccgtc
     3781 gggtggttgt gagtttcgac tttcatcaga atatgcgccg gttcctgatg gaaatcgtag
     3841 cggcccgttt cgtggtcagc aaagtagcgg cccacttcag aaccttccat tacggcggcg
     3901 ttatctttat aagcagagag aacgtgatct ggcgtggttt cgaaagtatt tttgatcatc
     3961 ctgaacagcg atttcggctg ttgttcaccg tcgataatcc agtcggcgtt aaaaattttg
     4021 tggcggcagt gctcagagtt cgcctgagcg aacatataca gctcgatgtc gttcgggtta
     4081 cgaccaagct ttgtgaaagc atcctgcaga tagtcaattt catcttccgc cagagccagg
     4141 ccaagacgca ggttagcgtc gatcagcgcc tgacggccct gccccagcaa atcaacgctt
     4201 gtgaccggag tcggttgatg gtgggcaaac aactgctctg catcatctaa atcaaaaaag
     4261 accgtttcca tcatgcggtc gtgcagttca gcggtaacct gctgccattg ttcattggtc
     4321 agcgtaccgg cttctatata gtaagcaacg ccgcgctcaa ggcggtttac ctgttgtagc
     4381 ccgcagttat gggcaatatc ggtcgctttc gaagaccagg gagagatggt gccaggacgc
     4441 ggggtcacca gcaggagttt gccttgcggg gcgtggctgg cgagtgccgg accatatttc
     4501 agcaggcgtt caagttgtgc gtgctcatca tcgtttaacg gcgcattgag gtcagcaaaa
     4561 tggacatact cggcgtaaat attgtgaacc gggagcctgg cagcctgaaa acgtgccagc
     4621 agtttgttga ttcggaatgc cgacagtgca ggcgaaccac gcagaatttc catcataagt
     4681 ctctcgtctt ctaagctttc ggtgtaccca agggggggaa acgggcgtca ttataaagaa
     4741 tctgatgcgc tgacgaaacc gtttgcgtgg aaataaaatc accatcgtga attagcaacg
     4801 cgtgccgcca atggatgtaa taagttgcta tctggcgcag gtttacgcaa aatgccgctc
     4861 atttatgagt aaacctttca ctattattac gttttttcaa gctgggacgc gcacgacaca
     4921 gagaattaac taattgaaaa aattaaagat taattatctg ttcatcggca ttctggcact
     4981 gctgctcgcg gtcgctctct ggccatccat tccctggttt ggtaaagccg acaaccgtat
     5041 cgccgccatt caagcgcggg gagagttgcg tgtgagcacc attcatactc ccctgactta
     5101 taacgaaatc aacgggaaac cttttggcct ggattacgaa ctggcgaaac agtttgccga
     5161 ttacctcggc gtaaaactga aagtgaccgt gcggcagaat atcagccagc tgtttgacga
     5221 ccttgataat ggtaacgccg acctgctggc ggcaggactt gtctataaca gtgagcgggt
     5281 aaaaaattat cagcctggcc ctacctatta ttccgtgtca caacaactgg tttataaagt
     5341 gggtcagtat cgcccacgta cgctgggcaa cctgacggcg gagcaactca ccgttgcacc
     5401 gggtcatgtg gtggttaacg atctccagac cctgaaagaa acaaaattcc cggaattaag
     5461 ctggaaggta gacgacaaaa aaggctctgc ggaattaatg gaagatgtca tcgaaggaaa
     5521 actcgattac accattgctg attctgtcgc catcagcctg tttcagcgcg ttcacccgga
     5581 gctcgccgta gcgctcgata tcaccgatga acaaccggtg acttggttta gcccgttaga
     5641 tggcgataat accctttccg ccgccctgct cgacttcttc aacgaaatga atgaagacgg
     5701 tacgctggca cgcattgaag agaaatacct ggggcatggc gatgattttg attacgtcga
     5761 tacgcgcaca tttttacgcg ccgtcgatgc ggtactgccg cagttaaagc ccctgtttga
     5821 gaaatacgcc gaagaaattg actggcgttt gctggccgct attgcttatc aggaatcgca
     5881 ctgggatgca caggccactt caccgacggg tgtgcgcggc atgatgatgt taaccaaaaa
     5941 taccgcgcaa agcctcggca ttacggatcg taccgatgcc gaacagagca tcagcggtgg
     6001 cgtgcgttat ttgcaggata tgatgagtaa agtgccggaa agtgtgccgg agaacgagcg
     6061 gatctggttt gccctcgctg cgtacaatat gggctatgcg catatgctgg atgcccgcgc
     6121 cctgacggca aaaaccaaag ggaatcctga cagttgggct gacgtaaaac agcgtctgcc
     6181 tttacttagc cagaaaccct attacagcaa gctgacttac ggctacgctc gtggacatga
     6241 agcctacgct tatgtcgaaa atattcgtaa gtatcagatt agcctggtgg gttatctgca
     6301 agagaaagag aagcaggcta cagaagcggc gatgcaactg gcgcaggatt atccggcggt
     6361 atcgcctacg gagttgggca aagagaaatt tccttttctc tcgtttcttt cccagtcgtc
     6421 atcaaactat ttgacccatt ctccctctct gctgttttcc aggaaaggga gtgaagagaa
     6481 acaaaattaa tccgtcgagg attgcgcttt tttctgcgct ttaatttcct ggcggcgcat
     6541 gcgaaagaag tcactgagca acgccgcgca ctcatccgcc agtattcctt ccgtaatttc
     6601 cactcggtga ttcatacccg gatgatgcag cacatccatt aaagatcccg cagcgccagt
     6661 tttcgcgtca cgcgcaccaa agaccacgcg accaatgcga ctgtggatca tcgctccggc
     6721 acacattaca catggttcaa gcgtgacata caacgtggcg tcgatcagac gataattttg
     6781 catcaccaga ccaccctgcc gcagggccat gatttctgca tgtgcggtgg gatcatggcg
     6841 accaatcggg cggttccagc cttcgccgat tacccgattg ttatgcacta ataccgcgcc
     6901 gaccggcact tcccgctcat cccaggcacg tttcgccagc gtcagcgcgt gacgcatcca
     6961 gtattcgtgg ctaaattcga cttcagacaa aaagaaaact ccggttataa aagcgcggcg
     7021 cattatacac ggacgctatg ctttactcta ttccagttgc tggagttcac cgcgcggggt
     7081 tactcgccaa cgatgctgac agaaataaag cagcggattg tcctgattac tgtcgctata
     7141 gccactgtac agccgcagcg gagtgccgat tttgcgctcc agttgtgcga ccttttcatg
     7201 tcccagacaa cgcatcgtca atacccaacc accatagcca cgctgaattt ggctggcgat
     7261 aagattaacc cgcggcagcc agggcgtatc gaaataaacc gcttcaacca gcggctgcgg
     7321 agagccggta atcaaccaga tatcagcatc ggaacttaac aggtaggtgg ttaatcgctc
     7381 ctgaaccagc ggaaaggcgg taacattgtc gcgaaaccag cgcacgaaat cggcctgcaa
     7441 cgtctgtaaa cgtgcttcgc tgtgaccaaa agtgcacccc cacagaagca gactcatcgg
     7501 ccagcgtgcc gcacgacctt ttatcaataa cgcaatggct ataatcggta acaacggcag
     7561 gacaagtaac gcattcagcg gttggcgacg cagtaaatag cgcagaaaac tgccgaacat
     7621 atcctgctga tgcaatgttc catctaagtc aaaaaacacc acacgacgct cgtgagttgc
     7681 caaaccatac tcctctggac tgaaacatcc tgattaattt tcgctactac atagcctaac
     7741 agatagatca tcacttttcc ggcaacaatc gaccgcatca tggctaactg gaatttttaa
     7801 ttcatggcaa ttagcggcaa tggaatataa aattcactcg cgtgtctctc atattgcctg
     7861 ttgtcgccac tatttcatcg cgaggaaaga tatgaacggc ttacttcgta tccgtcagcg
     7921 ttaccagggg cttgcccaga gtgataaaaa actggcggat tatctgctgc tacaacctga
     7981 tacggcgcgc catttaagct ctcagcaact ggccaacgaa gccggagtca gtcagtccag
     8041 cgtcgtgaag ttcgcgcaaa aactcggcta taaaggtttt ccggcgctta agttggcgtt
     8101 gagtgaagcg ctggcaagcc agccggaatc gccctccgtg cccattcata accaaatccg
     8161 cggtgatgat ccgttacggc tggtcggcga aaaactgatt aaagaaaata ctgccgctat
     8221 gtacgcaacg ctaaacgtta atagtgaaga gaaactgcat gaatgcgtaa caatgttgcg
     8281 ctctgcgcgg cggataattc tgaccggtat tggcgcttcg ggtctggtgg cgcaaaactt
     8341 tgcctggaag ctgatgaaga ttggcttcaa tgctgccgca gtgcgcgata tgcatgcgct
     8401 actcgcaaca gtacaggcgt cgtcccctga cgatctgtta ttagccattt cctacaccgg
     8461 tgtacgacgc gagttaaacc tggcggcaga tgagatgctg cgagtgggcg gaaaagtgct
     8521 ggcgattacc ggctttactc cgaatgccct gcaacagcgt gcttctcatt gcctgtatac
     8581 cattgccgaa gaacaagcga caaacagcgc ttcaatctct gcttgtcacg ctcagggaat
     8641 gttaacggat ttgctgttca ttgcgctgat tcagcaggat ctggaactgg caccagaacg
     8701 tattcgtcat agtgaagcgc tggtgaaaaa actggtctga gtaaagaatg cgcgtataat
     8761 gcccgcccgg tttgtgttgt tttgagagtt tccttatggc gttgttaatc actaaaaaat
     8821 gcatcaattg tgatatgtgt gaacccgaat gcccgaatga ggcgatttca atgggagatc
     8881 atatctacga gattaacagc gataagtgta ccgaatgcgt agggcactac gagacaccaa
     8941 cctgccagaa ggtgtgcccg atccccaata ctattgtgaa agatccggcg catgtcgaga
     9001 cagaagaaca gttgtgggat aaatttgtgc tgatgcacca cgcggataaa atttaatatt
     9061 ctactctgga agtagagtat taattatatt actgggaagc cttaacgcca ttatatttat
     9121 ttaattgatg acattagcat aatcattcac taagttaatt tatatagtat ctgccaagac
     9181 acttatttat agttattaaa ggcgcgtccg attggttcac cggacgcacc ttaagtacgt
     9241 ttccttgtgt tataagaaca gaaggatcag ctgtaaaaca gcaatgatga ttttgatgac
     9301 ccgtttaatc aggtatcggc aatcagtcat tcgtttttcc ttaaacaagg aatgcagccg
     9361 ttcagattag cccttacatc tccccaaaac tgaacgtgcg agttattgag ggtgcatgct
     9421 gcactccaca ccagagcttt gacgacacca ctcgtttcaa tgggggaatt ctgtggcatg
     9481 gtgtaaagca cagcaaaatc ttcaataacg aagccaattt taatgtactt acgaattggc
     9541 ggtcacgtta atctttccat caatattgct ttcttcgtaa aggctcgagt ttttatgcta
     9601 aagattgcaa gttgcttgta aaagataagt acactgatcc ataatcgctg ttgttgaggg
     9661 tgcatgctgc acaaaattaa agttaaaaag taaaaccccc gttccttacc agttcggggg
     9721 ttttactttt taaagagaac ggtattattt ttaactttca ataattaccg tggcacaagc
     9781 atagtgccgc tcatctgcca gcgttacatg catatttgca acgcccagct tttccgccag
     9841 ttttaatgcc tcgccccata gccgtagccg tggtttgccg agctcatcat tgaatacttc
     9901 aaattgatta aacgccagac cattgcggat cccggtgcca aacgcttttg ctgcggcttc
     9961 tttcacagca aaacgcttcg ccagaaaacg caccggctgg tggtgcgttt tccagatagc
    10021 ccattcgtta tcgcttaata cgcggcgtgc caggcgatca ccggatcggg cgatcaccgc
    10081 ttcgatgcga gcgatctcca caatatccgt gcctaaacct aatattgcca ttagccacgc
    10141 gcttccagca tcagacgctt catttctgcc accgcatctt tcagtccggt catcactgca
    10201 cgaccaataa tggcatgacc gatattcagt tcatgcatct cagggatggc ggcaatggct
    10261 ttcacgttgt gataggtcag accgtgtccg gcgttaactt tcagaccgag gcttgcggca
    10321 aaggtcgcgg ctttggcgat acgcgccagc tcttgcgcct gttcggcgtc agttttggca
    10381 tcagcatagc aaccggtgtg gatctcgata aacggtgcgc caacctctgc cgcagctttg
    10441 atctgctctt catcggcgtc aataaacaga gaaacctgaa tcccggcatc tgccagacgt
    10501 ttgcaggcat cgcgcatttt gtcacgctgc cctgcgacat ccaggccgcc ttcggttgtt
    10561 acttcctgac gcttttccgg taccaggcag caaaaatgtg gcttcgtctc aacggcgatc
    10621 gccagcatct cttcggtcac cgccatctcc agattcatgc gggtatccag cgtctgacgc
    10681 aggatgcgca cgtcgcggtc agtaatgtga cggcgatctt cacgtaaatg cacggtaatg
    10741 ccgtccgctc ccgcctgctc ggcaataaac gcggcctgca ccggatccgg gtaagcggta
    10801 ccgcgcgcgt tgcgcagcgt agcgatatgg tcaatgttga cgcctaacag taattcagcc
    10861 atgacaatcc tcatcattca taatgtgttt tcaccgttcg cttaggcata aattgccgga
    10921 acagttccct gctctttaaa ggtttaccgc caagatacgg cttaagcgcc atgcgggtaa
    10981 agcgtttcgc agcgcgcagt gtgtctgcgt caggaaattc ccgtgcgttt aacgctttta
    11041 actgccttcc ggtgaacgtt ttattgtcga taacgacgct tgcgataaac cctttttctt
    11101 cgcgataacg atacgtcatg gtgtcatcta ccggctcgcc gctacccgca caatgggtaa
    11161 aattgacgcc ataacccaga tgcccgagca gtgccagttc aaagcggcgc agcgcgggtt
    11221 ctggcgtacc agtgacccct gcaagagact gaatgcagtg caagtaatcg aaaaaaagtt
    11281 cagagaagcg cgtctcgtat tccagtacgc gggagagaag ttcgttgatg tacagaccgc
    11341 tgtaaagcgt gataccgctt aatggcagcg ccagcgagac ggcttcagca ctgcgcagcg
    11401 ttttgacttc gccacgcccg ccaaaacgta gcaagagagg ggtgaaaggc tgtaatgcac
    11461 ctttcagggt agagcgttta gagcgtgcgc ctttggcaac cagacgcacg cgccccgatt
    11521 cctccgtgaa gacgtccagc atcaggctgg tttcgctcca cgggcgacta tgcaggacaa
    11581 atgcgcgctg ccagccttcc atcggagtta ctcttaaaga tcgtcaacgt aaccgagact
    11641 gcgcagtgcg cgttcgtcgt cggcccaacc ggatttcact tttacccaca gttcaaggtg
    11701 aacaggcgct tcgaacattt cctgcatgtc tttacgcgct tcaatcccga tggttttgat
    11761 cttggcccct ttgttgccaa tgaccatctt cttctgccct tcacgctcaa cgagaatcaa
    11821 accgttgatg tcataaccac cgcgttcgtt agagacgaaa cgttcgatct ccacggtcac
    11881 ggagtacggc agttcagcgc cgaggaaacg catcagtttt tcgcggatga tttcagacgc
    11941 cataaaacgc tgtgagcgat cggtgatgta atcttccggg aagtgatgag tcgcttcagg
    12001 tagatgctta cgcacgattg ccgcaatagt gtcaacattc agcccggttt cggcagagat
    12061 tggcacgata tcgaggaagt tcatctggct tgccaggaac tgcaggtgcg gcagcagatc
    12121 ggctttctcc tgcacgttgt ccactttgtt caccgcgagg attaccggcg ctttgccttc
    12181 gcgcagtttg ttgagcacca tttcgtcgtc cggcgtccag cgggtgcctt caacgacaaa
    12241 aatcaccagc tcaacatcgc caatagagct gctcgccgct ttgttcatca ggcggttaat
    12301 ggcgcgtttt tcttccatat gcaggcccgg tgtatcgacg tagatcgcct gatacgcgcc
    12361 ttcagtatgg atccccacaa tgcggtgacg agttgtctgc gccttgcggg aagtgatgga
    12421 gattttctgc cccagcagtt tgttcaacaa tgtggatttg ccaacgttcg gacgtccgac
    12481 gatggcaata aatccgcagt aacttttatc gatgctcatt ccagctccag ttttttcaac
    12541 gcctgttcgg cggcagcctg ctcagcctta cgacggcttg aacctgtgcc aaccaccggt
    12601 tcactcaggc cgctgacctg gcagtggata gtaaattcct gatcgtgcgc ttcgccacgt
    12661 acctggacta ccagataagt cggcagcggc agatggcgac cctgcaaata ttcttgcaag
    12721 cgcgttttcg gatctttttg tttatcgcct gggctaattt cgtccaaacg agtttgatac
    12781 cagttgagga ttaatttctc gacggtttga atatcactgt cgaggaatac gccaccaatt
    12841 aatgcttcga cggtgtcggc gagaattgac tcacgacgaa atccaccgct tttaagttca
    12901 cctggcccta aacgtaagca ctcgcctaac tcaaattcgc gcgccagttc cgccagcgta
    12961 ttgccacgga ccagcgtggc gcgcatccgg ctcatatcgc cttcatccac acgagggaaa
    13021 cggtgataaa gcgcattggc gataacgtag ctcagaatag agtcgcctaa aaattctaaa
    13081 cgctcgttat gtttactgct ggcactacga tgagttaatg cctgctgcaa cagttcctga
    13141 tgattaaaag tgtagcccag cttccgttga agccgattaa ttacgatggg gttcatgcga
    13201 taccaataaa taaatgcgtc aacaattcag cacacgaaac agacctgata tacatggttc
    13261 tgctaactgc ttcgctgcag tttcagttta ccggtatatg gggaccaacg ctgtttcgtg
    13321 tgccgtggca acctggaggt gccaacctta aacttcgggg gaatattcta tacacaacga
    13381 cgggggatgt cgttagccac gggagattta tctcataaat aattcacgtt gtcgccataa
    13441 cggcgacaac gtgaacgaag atggctatta atggatgccg ccaatgcgac ttaagcgcag
    13501 accagtcggc cattcgcctt cttgcttatc gaagctcatc cagatagccg ttgcccgacc
    13561 gaccagattc gcttccggca caaagcccca gtaacggctg tccgcgctgt tgtcgcggtt
    13621 gtcgcccatc atgaagtatt gtcccggagg aacaatccag gttgccagtt gttgccctgg
    13681 ctgctggtaa tacatcccca cctgatcctg cgcaatcggc actgtcagaa tgcggtgcgt
    13741 cacatcaccc agtgtctctt tacgctcgga aagacgaatt ccattttctt tggtttcgtt
    13801 tttcggcact tcaaagaatc cgctggtcgc ttccccacca ttacggcgtg agaaggtctg
    13861 aacgaaatcg ctcggttcca cgtttgagta ggtgaccggc agcgcgtttt cacacgcctg
    13921 gccggaactg catcccggtt gaatcgtcag ctcttttgag accggatcgt aagtgacttt
    13981 atcgcccggt aaacccaccg cgcgcttgat gtaatcaagc tttggatctt ccggatattt
    14041 aaagaccacg atatcgccgc gtttcggatg accggtttcg atcagcgttt tctggtagat
    14101 aggatcttta atgccataag caaacttctc taccagaata aaatcaccaa ttaacagagt
    14161 cggcatcatc gaacctgacg ggatctggaa cggttcataa ataaacgaac gcacaatcaa
    14221 tacgatagcc agtaccggaa aaacagaagc accggtttcc agccagccag gcttcggcgc
    14281 aacctttttc aacgttgctt tatccagtga gtcaccggca gccgcctgcg ccgctgcctg
    14341 acgttcccgc cgtttaggtg cgaaaaagaa tttatccacg caccataaaa tgcccgtcac
    14401 cagtgtggca atcaccagaa tcagggcaaa catattcgcc atgccaactc ctaagggtta
    14461 tttgttgtct ttgccgacgt gcagaatggc gaggaacgct tcctgcggca gctcgacgtt
    14521 accgatctgc ttcatgcgtt tcttaccttc tttctgcttc tgcagcagct ttttcttacg
    14581 gctgatatcg ccgccataac atttagccag tacgttttta cgcagctgtt tcacggtgga
    14641 tcgcgcaata atgtgcgtac caatcgctgc ctgaatggcg atatcaaact gctggcgtgg
    14701 gatcagatct ttcatcttct ccaccaactc gcgaccgcgg ttttgcgaat tatcacggtg
    14761 ggtgatcagc gccaacgcat caacacgttc accgttgatt aatacgtcta cacgtaccat
    14821 gtcggacgcc tggaagcgtt tgaagttgta atccagagac gcataaccac gcgaggtaga
    14881 tttcaggcga tcgaagaagt cgagcaccac ttccgccatc gggatctcgt acgtcagcgc
    14941 cacctgatta ccgtggtaaa ccatattggt ctgcacgccg cgtttttcta cgcacagcgt
    15001 aataacgttg ccgagatatg cctgcggcag cagcatgtga cattcggcaa tcggctcgcg
    15061 cagttcgtag atgttattta ccgctggcag cttggatgga ctgtcgacgt aaataacttc
    15121 tcttgacgtg gtttcaactt catacactac ggtcggcgca gtggtgatca gatccagatc
    15181 gtattcacgt tccagacgtt cctggatgat ctccatgtgc agcaggccga ggaagccgca
    15241 gcggaaacca aagcccagcg cgctggagct ttccggctca tagaacagtg aagcatcgtt
    15301 caggctgagt ttacccagcg cgtcacggaa ggcttcatag tcgtcggaac ttaccgggaa
    15361 cagaccggcg tatacctgcg gtttgacttt cttaaagcca ggcagcgcct tttctgccgg
    15421 attacgcgcc agcgttaagg tatcgccgac tggagcgccg tggatatctt taatcgcaca
    15481 taccagccag cctacttcgc cacatttcag ttcagtgcgg tcaacctgct tcggcgtgaa
    15541 gatgcccaga cggtcggcgt tataggtctg cccggtactc atgactttca ctttgtcgcc
    15601 cttacgcagg gtgccgtttt taatacggat aagtgaaaca acgcccagat agttgtcgaa
    15661 ccaggagtcg ataattagtg cctgcaacgg gccttccgga tcgccttccg gcggcggaat
    15721 gtcgcgcacc agacgttcga gaacgtcctg cacgccaacg ccggttttcg ctgaacagcg
    15781 caccgcgtcg gtggcgtcga tgccgacgat atcttcaatt tcttccgcca cgcgttcagg
    15841 atcggctgcc ggcaggtcaa tcttgttcag taccggcacc acttcgagat ccatttccat
    15901 ggcggtgtag cagtttgcca gggtttgcgc ttctacgccc tgcccggcgt cgaccaccag
    15961 caatgcacct tcacaggcag ccagcgaacg ggaaacttca taggagaagt ctacgtggcc
    16021 cggggtgtcg ataaagttaa gctgataggt ttcgccgtca gacgctttgt agtccagcgt
    16081 cacgctttgc gctttgatgg taatgccacg ctcacgctca agatccatgg aatcgagaac
    16141 ctgcgcctcc atttcacggt cagacaggcc accgcagatc tggataatac ggtcagacag
    16201 cgtcgattta ccgtggtcaa tgtgagctat gatcgaaaag ttacgtatat tcttcataaa
    16261 gtatgattat tgtgccttaa tgcccggtta accaggcttt tagaagtcgc tgttctgagc
    16321 ttaacgtctg tattaataga aacgccgcat tctacactac aacattgagg cgaggaaatg
    16381 ttcataccgt atggattgtg gtatctggaa acgtcctcgc atttgttatg caaaatgcaa
    16441 taaagccagt gaaatcactg gctcgcgtct tccgaagatg tttcaaatcg cacaaggcca
    16501 ggcggcaatg ccacgcttaa gatgatcggt tgccattctg cccgggcagc aaacttacgc
    16561 gagtagccgc gcgcaatcag gaacccgcca ataccaccga ggatcgcacc acataatgct
    16621 gcgacgtcag aagcaaagag tagctgaaat agcgaagcga tgaggaataa tcccaccagc
    16681 ggcgacatat aaaccagtaa tgcggagcta agcaggctgc cttcggcgat ccccaattcc
    16741 actttttgcc ccggcactaa cggctcatca cagggtacga caatggtatg cgtggtttgc
    16801 gggccaagtt tatttaacac gcggctaccg caaccggcgc gtgaagcgca gctgctgcat
    16861 gaagctttaa catcacaact gaccagcgcc tgcccgtttt gccaggagac gacggtagcc
    16921 cactctttga tcattgcgct gccccgaact taatattctc ggcaatgcgt ttcgccgttt
    16981 gcggcggcag ttcaccgaca atggtgattt cggcgttatc acgtacgctt gtactgacgg
    17041 ttctgcgtcc ggtgcgcaac atctgatcgg tgctcgatgg cgtagcgcgg ttaacgttta
    17101 ccgagaagct gaataatccg tcggaataga gacgtgattc gataggcatg ttgtccatcg
    17161 tcggtagcgg acgtcgacta ctggaaactt cgctaaaacc ctgtggcaac caggttggcg
    17221 tccagctgaa tttagctttt tcacctacag gaacagaaag caacggcggc aaatttgcct
    17281 tcgccagcgt ctgcatactg ctgctgatat cctgattgac gttaaaagca atcacgcgaa
    17341 attgttccag cgtttcacca tcgcgatcaa ggagatcaac ccgcatcggt aatttcgatt
    17401 cggtgtccat ccacacgatg tagctgtagc gtgtaccatc tcgggcaacc acgcgaatga
    17461 cttcgcaaag acgatcagca atacgcgtgc gcccgacgga gataaagtcg tagtaaggag
    17521 aaaggcgttt gaaatcggta tagataagcg atggcagaga atcaacaatg taatcgccat
    17581 taagcgtgaa cggttcaagt cccggttcaa aatagctgat ttcattgccg cgctgtacca
    17641 cttcccggcg cgggccatcc atttgcaaca attgtgcaag agggcggtta tcgaggcgtg
    17701 catgtcgata acgcagagac tcaacaccct gtttattgat gctgatgaat gacagctcgt
    17761 aattcagtga ctgactggcc aggttcatct gctgtaataa cgccccggac gcgggagtgg
    17821 ccgaggcgtt agcagagaat aacaggctac ctgtcactaa tgacatggca aaccaaagtt
    17881 gcttcattac tgcgattgcg ttcctaaagt ttgaattcct ggcacctgta cagcggcttg
    17941 ctgggtttgc gcctgctcaa actgaagctg ttcagagtgg agtcggcgtt gcagttcgta
    18001 atcctgcaac attgcattaa tgcgacgacg ctgctcctgt acctgctgct gttgaccatt
    18061 gtttgcggtc gcttcagaag gtactcccag gcttaccggg ctggctttac ccatcatcgg
    18121 cagtgtatta aataccggcg tttcgggctg ctgggacgtt tcagattgtc cattatagtg
    18181 ctggacgcca acgataactg caagcgatac gcatgctgct acgcccattt gggtaagctg
    18241 tgccgcccac ggacgtactt tctgccagaa tggcattttc tgccattgat gcggcgcagg
    18301 ctgggcttcc gggatcaatg tcgccggttg acgtactggc tcttcttcaa tggcggccat
    18361 cacgcgtgaa gagatatcga aatggagcac ctcgggagta tcaccccgca ttgagtcacg
    18421 gattaagtga tagctttccc aggttttctg catttctggg ttatgagcca gttcgttaag
    18481 cagctcacta tccagcgttt cgccatccat taaagcggaa agttgttctt tctgcatgcc
    18541 taataccctt atccagtatc ccgctatcgt caacgcctga taagcggttg aactttgtta
    18601 tcaatagctt ccctcgctcg gaagatacgt gaacgcaccg tacctaccgg acaatccatg
    18661 atagcggcta tctcttcata gctcaggcca tccagctccc gcaaggttat tgccatgcgc
    18721 aaatcttccg ggagggactc aatagttcgg aaaactatct gtctcagttc ttctgacaac
    18781 attaagttct cagggttcga aatttctttc aacgcgccgc cactttcgaa gttttcagct
    18841 tcaatggcat ccacatcact ggaaggtgga cgacgcccct gagcaaccag gtaatttttc
    18901 gctgtattta cagcaatccg atacagccat gtataaaaag cgctatctcc ccggaacgaa
    18961 tccagcgcac gataggcttt aataaaagct tcttgtacca catcgggaac atcacccgac
    19021 ggcacatagc gggaaaccag actcgccact ttatgctgat agcgcactac cagtaggtta
    19081 aaggctttct gatctccctt ctggacccgt tcaaccagga cctggtccgt taactgctcg
    19141 ctcatccgag gtaaagtctc cccaaaccaa atttccacgc gctatcgaaa cgccactcca
    19201 ttagctgcaa tttgagcaag caaagggtta gagtgtctcg tttttgtaaa gttccgtaac
    19261 gcatctgttt ttgtttgtca tgctgtagac ggatcattat ctatcattat aagtctacag
    19321 aatctgaaca tcgcattatc tgtgtagaaa tgcccattta actgcctgaa gagtaaccca
    19381 acggcctttt tatttcacca cctaatcctc caccagccag taacttctct ttttctcgcc
    19441 gccctgcgtc agcgtgttta gccactgtaa caaatattaa aatagcaggt gtttattcgc
    19501 acaacatgat gctatgctga ccaaacggtg tttagtaaat taaacaaaga aaatgaatac
    19561 tctcccagaa cattcatgtg acgtgttgat tatcggtagc ggcgcagccg gactttcact
    19621 ggcgctacgc ctggctgacc agcatcaggt catcgttcta agtaaaggcc cggtaacgga
    19681 aggttcaaca ttttatgccc agggcggtat tgccgccgtg tttgatgaaa ctgacagcat
    19741 tgactcgcat gtggaagaca cattgattgc cggggctggt atttgcgatc gccatgcagt
    19801 tgaatttgtc gccagcaatg cacgatcctg tgtgcaatgg ttaattgacc agggggtttt
    19861 gtttgatacc cacgttcaac cgaatggcga agaaagctac catctgaccc gtgaaggtgg
    19921 acatagtcac cgtcgtattc ttcatgccgc cgacgccacc ggtagagaag tacaatccac
    19981 gctggtgagc aaggcgcaga accatccgaa tattcgtgtg ctggagcgca gcaacgcggt
    20041 tgatctgatt gtctctgaca aaattggcct gccgggcacg cgacgggttg ttggcgcgtg
    20101 ggtatggaac cgaaataaag aaacggtgga aacctgccac gcaaaagcag tggtactggc
    20161 aaccggcggt gcgtcgaagg tttatcagta caccaccaat ccggatattt cttctggcga
    20221 tggcattgct atggcgtggc gcgcaggctg tcgggttgcc aatctcgaat ttaatcagtt
    20281 ccaccctacc gcactgtatc acccacaggc acgcaatttc ctgttaacgg aagcactgcg
    20341 cggcgaaggc gcttatctca agcgcccgga tggcacgcgt tttatgcccg attttgatga
    20401 gcgcggcgaa ctggccccgc gcgatattgt cgcccgcgcc attgaccatg aaatgaaacg
    20461 cctcggcgca gattgtatgt tcctcgacat cagccataag cccgccgatt ttattcgcca
    20521 gcatttcccg atgatttatg aaaaattgct cgggctgggg attgatctca cacaagaacc
    20581 ggtaccgatt gtgcctgctg cacattatac ctgcggtggt gtaatggttg atgatcatgg
    20641 gcgtacggac gtcgagggct tgtatgccat tggcgaggtg agttataccg gcttacacgg
    20701 cgctaaccgc atggcctcga attcattgct ggagtgtctg gtctatggct ggtcggcggc
    20761 agaagatatc accagacgta tgcctgatgc ccacggcgtc agtacgttac cgccgtggga
    20821 tgaaagccgc gttgagaacc ctgacgaacg ggtagtaatt cagcataact ggcacgagct
    20881 acggctgttt atgtgggatt acgttggcat tgtgcgcaca acgaagcgcc tggaacgcgc
    20941 cctgcggcgg ataaccatgc tccaacaaga aatagacgaa tattacgccc atttccgcgt
    21001 ctcaaataat ttgctggagc tgcgtaatct ggtacaggtt gccgagttga ttgttcgctg
    21061 tgcgatgatg cgcaaggaga gccgtggact gcattttacg ctggattacc cggaactgct
    21121 cacccattcc ggtccatcga tcctttctcc cggcaatcat tacataaaca gataaaaagc
    21181 ctgggtcagc gctgtatacg cttcggaata gttctggtct ggcccacgaa tgactaagcg
    21241 atcactaaag cattctcccg cctgcgggga gaatgccaac agcacccgat gcggcagtcg
    21301 cgcttcgttt tccgccacat ccgtccgcaa acgtaaatgc cagcccatgc ttaatgccag
    21361 ttcagtaaaa ccattaccaa tctgctctgg cagcaccacg cagaaaaatc cctcttcggt
    21421 aatgcactcc gccgcacagg tcagcagcga tgggtgatca agcgtagtgg tatagcgagc
    21481 ctgttcccgt tgaggtgtcg cacactctac tccctgctga tagtaaggtg ggttactgat
    21541 gattaaatcg aagcgcgcgg tctgctgtgt tacccactgc tgaatatccg ccgtatggac
    21601 gttaatccgc tctgcccacg gggactggtt gatattttcc tgcgcctgcg cggcagcttc
    21661 actttccagc tcaacggcat caattatcac gctgtcactg gttcgctgcg ccagcatcag
    21721 tgccagcaac ccgctacccg cgccgatatc aaggcaacgt ttcaccccag ccaccggtgc
    21781 ccatgcgccc agcaaaatgc catccgttcc cactttcatc gcacagcgat cgtgagcaac
    21841 aaaaaactgt ttaaaagtaa atccattacg acgaagcacg gatgtagact gtgacatgaa
    21901 aataaaacct tgcaggaaaa acggcgatag caccgggtga gaacaatacc cgagaagcga
    21961 tatccataca aacagatgaa gattgcagcc gtaacgtcta taatcagcgc cccacacaga
    22021 ggtagaacat gactgtaacg actttttccg aacttgaact cgacgaaagc ctgctggaag
    22081 ccctccagga taaaggtttc actcgcccga ccgccattca ggctgccgcc attccgcctg
    22141 cgctcgatgg ccgtgatgta ctcggttctg cgccgacagg caccggtaaa acggcggcgt
    22201 atctgctgcc agcgttgcag cacctgctcg atttcccacg taagaaatcc ggtccaccgc
    22261 gtattttgat cctcacccca actcgcgagc tggcgatgca ggtggccgat catgcccgcg
    22321 aactggcgaa acatacgcat ctggatatcg ccaccatcac cggcggcgta gcctatatga
    22381 accacgcgga agtgttcagc gaaaatcagg acatcgtggt cgccacgacc ggacgtctgc
    22441 tgcaatacat aaaagaagag aacttcgatt gccgcgcggt tgaaacgctg atcctcgacg
    22501 aagcagaccg tatgctggat atgggcttcg ctcaggatat cgaacatatt gctggcgaaa
    22561 cgcgctggcg taaacagacc ctgctctttt cggcaacgct ggaaggcgat gcgattcagg
    22621 actttgccga gcgtctgctg gaagatccgg tggaggtttc tgccaatccg tccacccgtg
    22681 agcgcaaaaa aattcatcag tggtattacc gcgccgatga cctggaacat aaaaccgcat
    22741 tgctggtgca tctgttaaaa cagccggaag cgacccgctc aattgtgttt gtgcgtaagc
    22801 gtgaacgtgt gcatgagctg gcaaactggc tgcgcgaagc gggcatcaac aactgctatc
    22861 tcgaaggtga gatggtacaa ggcaagcgta acgaagcgat caagcgtttg accgaaggtc
    22921 gcgtaaacgt actggttgca accgatgttg ccgcgcgcgg tatcgacatt cctgacgtca
    22981 gccacgtctt taacttcgat atgccgcgca gtggcgatac ttatttgcac cgtatcggac
    23041 gtaccgcgcg cgccggtcgt aaaggcaccg caatttcgct ggtggaagcc catgaccatt
    23101 tgctgctggg caaggttggc cgttacattg aagagccaat taaagctcgc gttattgatg
    23161 agttacgccc gaaaacgcgt gcgccaagcg aaaagcagac cggcaagcca tcgaagaaag
    23221 tactggctaa acgtgccgag aagaaaaaag ctaaagagaa agagaagccg cgggtgaaaa
    23281 aacgccatcg cgacaccaaa aatattggta agcgccgtaa accaagcgga acgggcgtgc
    23341 caccgcaaac gacagaagag taatctcaat gccgggttta agcctggtat taaaagtgct
    23401 ggcaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggct catgcaatat
    23461 attgaatttg cacgatcttg taggccggat aaggcgttca cgccgcatcc ggcatgaata
    23521 aagcgcactt tgtcgacaag ttaaatgccg ggtttaatcc cgacattatt tttatccagc
    23581 cacaaaactc tcttcaacac actgaataaa agtgtgcatc gccgggctaa ccgcttttcc
    23641 agcatggtgt gcgcacatgg ccgtaatggt ctgcgactgt tcgccaaaag gcaattcaat
    23701 taattctccg ctctcaagct ctttttccac cgcaaagcgg ggcaagtaac tgacaccgat
    23761 attcgccgcg acacaacgtt tgatactttc aatgcttaac agctcaatgg tgttttccac
    23821 cgtgatccgc cgctgacgca gcgtgctctc aaatatctgc cggaagacac attgcggttc
    23881 gttgataata aagctacagg cgttatgtct tcccggctca gtaaaatcga catctgcaat
    23941 ttgcggtgaa gccaccagca ccagcgattg ttcacccaac tctcgtcggt tcagggcatc
    24001 atcattccct acgcgataaa agacgccgac atcggcctca tcattcagta gcgcatcacg
    24061 gatcacgtaa cagttcagcg actgcaacga taaacgcact tttggtgcgc gttgccgaaa
    24121 acgctgcaac acctgcggca ttcggtaaga gagcaacgtt tcgcccgaaa caacgcgtag
    24181 ttccccgtcc ggatctgact cctttttagc agcctcacga agcgtatcca tcactcgggt
    24241 aagttcataa atgtgcggca gcaacttttt tccttcgcgg gtaaggcaca tccgtcggcc
    24301 aattttctca aataactgga ctgaaaactc ctgctcaagc tgctgaatat gaaaagtcac
    24361 cgtcgattgt gtacagcaca atttttgcga agctcgcaaa aaggaaccct cttccaccac
    24421 ggttttaagc gtaataaaac ggcgcagatc cataacccca aacctatcga aaatatcgaa
    24481 tctagaatat aaaaacattc atttttttaa atgttccgtg tcgggtactg tctaccaaaa
    24541 cagaggagat aacaagtgac accgaccctt ttaagtgctt tttggactta caccctgatt
    24601 accgctatga cgccaggacc gaacaatatt ctcgccctta gctctgctac gtcgcatgga
    24661 tttcgtcaaa gtacccgcgt gctggcaggg atgagtctgg gatttttgat tgtgatgtta
    24721 ctgtgtgcgg gcatttcatt ttcactggca gtgattgacc cggcagcggt acaccttttg
    24781 agttgggcgg gggcggcata tattgtctgg ctggcgtgga aaatcgccac cagcccaaca
    24841 aaggaagacg gacttcaggc aaaaccaatc agcttttggg ccagctttgc tttgcagttt
    24901 gtgaacgtca aaatcatttt gtacggtgtt acggcactgt cgacgtttgt tctgccgcaa
    24961 acacaggcgc taagctgggt agttggcgtc agcgttttgc tggcgatgat tgggacgttt
    25021 ggcaatgtat gctgggcgct ggcggggcat ctgtttcagc aattgtttcg ccag
//
LOCUS       DAMZET010000055        24107 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.055, whole genome shotgun sequence.
ACCESSION   DAMZET010000055 DAMZET010000000
VERSION     DAMZET010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.055"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..658)
                     /locus_tag="POB34_002259"
     CDS             complement(<1..658)
                     /locus_tag="POB34_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="involved in the post-transcriptional processing of
                     the daa operon mRNA, which encodes proteins involved in
                     fimbrial biogenesis of an enteropathogenic E. coli strain;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HDI5919196.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     R"
     gene            859..1464
                     /gene="azoR"
                     /locus_tag="POB34_002260"
     CDS             859..1464
                     /gene="azoR"
                     /locus_tag="POB34_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDI5919197.1"
                     /translation="MSKVLVLKSSILAEYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LVFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(1515..2831)
                     /locus_tag="POB34_002261"
     CDS             complement(1515..2831)
                     /locus_tag="POB34_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HDI5919198.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(2821..4578)
                     /locus_tag="POB34_002262"
     CDS             complement(2821..4578)
                     /locus_tag="POB34_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HDI5919199.1"
                     /translation="MENSRIHGEHFFTSSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTVFYAWDARGHGQTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLDEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQA
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(4594..5490)
                     /locus_tag="POB34_002263"
     CDS             complement(4594..5490)
                     /locus_tag="POB34_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDI5919200.1"
                     /translation="MLEKSLATLFALLILATLINHFLLWRLPERKEDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLFIGFNC
                     FELYLLFIPLAGFLILATGQVFMGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(5490..6095)
                     /locus_tag="POB34_002264"
     CDS             complement(5490..6095)
                     /locus_tag="POB34_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDI5919201.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(6266..8572)
                     /locus_tag="POB34_002265"
     CDS             complement(6266..8572)
                     /locus_tag="POB34_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HDI5919202.1"
                     /translation="MFKSRICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSSIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(8636..9496)
                     /gene="pdxI"
                     /locus_tag="POB34_002266"
     CDS             complement(8636..9496)
                     /gene="pdxI"
                     /locus_tag="POB34_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HDI5919203.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRIMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(9728..10318)
                     /gene="paaY"
                     /locus_tag="POB34_002267"
     CDS             complement(9728..10318)
                     /gene="paaY"
                     /locus_tag="POB34_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HDI5919204.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKLIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPGRKRLVFDENLRPKQ"
     gene            complement(10300..11250)
                     /gene="paaX"
                     /locus_tag="POB34_002268"
     CDS             complement(10300..11250)
                     /gene="paaX"
                     /locus_tag="POB34_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HDI5919205.1"
                     /translation="MSKLDTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVQLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPSPGSLYFQRFGGLNIEQEALCQFIR"
     gene            complement(11351..12664)
                     /gene="paaF"
                     /locus_tag="POB34_002269"
     CDS             complement(11351..12664)
                     /gene="paaF"
                     /locus_tag="POB34_002269"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HDI5919206.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(12691..13896)
                     /gene="paaJ"
                     /locus_tag="POB34_002270"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(12691..13896)
                     /gene="paaJ"
                     /locus_tag="POB34_002270"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HDI5919207.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(13896..14318)
                     /gene="paaI"
                     /locus_tag="POB34_002271"
     CDS             complement(13896..14318)
                     /gene="paaI"
                     /locus_tag="POB34_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HDI5919208.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(14308..15735)
                     /gene="paaC"
                     /locus_tag="POB34_002272"
     CDS             complement(14308..15735)
                     /gene="paaC"
                     /locus_tag="POB34_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaC"
                     /protein_id="HDI5919209.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAANLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVATEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(15737..16525)
                     /gene="paaB"
                     /locus_tag="POB34_002273"
     CDS             complement(15737..16525)
                     /gene="paaB"
                     /locus_tag="POB34_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase"
                     /protein_id="HDI5919210.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(16525..17292)
                     /locus_tag="POB34_002274"
     CDS             complement(16525..17292)
                     /locus_tag="POB34_002274"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase"
                     /protein_id="HDI5919211.1"
                     /translation="MSELIVSRQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATD
                     TSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(17289..18359)
                     /gene="paaK"
                     /locus_tag="POB34_002275"
     CDS             complement(17289..18359)
                     /gene="paaK"
                     /locus_tag="POB34_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HDI5919212.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASLDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYARDHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIAATLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(18367..18864)
                     /gene="paaD"
                     /locus_tag="POB34_002276"
     CDS             complement(18367..18864)
                     /gene="paaD"
                     /locus_tag="POB34_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate degradation protein PaaD"
                     /protein_id="HDI5919213.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMSTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVCCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(18879..19625)
                     /gene="paaC"
                     /locus_tag="POB34_002277"
     CDS             complement(18879..19625)
                     /gene="paaC"
                     /locus_tag="POB34_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HDI5919214.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAIN
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(19634..19921)
                     /gene="paaB"
                     /locus_tag="POB34_002278"
     CDS             complement(19634..19921)
                     /gene="paaB"
                     /locus_tag="POB34_002278"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HDI5919215.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(19933..20862)
                     /gene="paaA"
                     /locus_tag="POB34_002279"
     CDS             complement(19933..20862)
                     /gene="paaA"
                     /locus_tag="POB34_002279"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HDI5919216.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            21147..23192
                     /gene="paaZ"
                     /locus_tag="POB34_002280"
     CDS             21147..23192
                     /gene="paaZ"
                     /locus_tag="POB34_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HDI5919217.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RLFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDEAPAVH
                     ATEAFGPVATLMPAQNQRHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLENLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            23440..>24107
                     /gene="tynA"
                     /locus_tag="POB34_002281"
     CDS             23440..>24107
                     /gene="tynA"
                     /locus_tag="POB34_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="catalyzes the formation of phenylacetaldehyde from
                     2-phenylethylamine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HDI5919218.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDKTL
                     KEFGADVQWDDYAQLFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFA"
BASE COUNT         6106 a         6491 c         5691 g         5819 t
ORIGIN      
        1 gccgcggcag caactctttc aaatagccga gcagaaaatc gatattcagg ctgcgttcgt
       61 gtgcttcgtc aataatgatg gtgtcgtact gcatcagcag gcggtcttgc tggatctccg
      121 ccagcaggat accgtcggtc atcagcttga ccatcgtgtt atcactaacg tgatcgctga
      181 aacgcacttt gtaaccgatg caaccgcccg gctccgtttt cagctcttcc gcaatacggt
      241 tcgccaccgt tcttgccgcc aggcgacgcg gctgggtatg gccgatcagc cctttaatcc
      301 cgcgccccag ctccatacag attttcggta actgagtcgt tttaccagaa cccgtttccc
      361 cggcgacgat caccacctgg tgatcacgaa tcgcttcgag aatgtcctgt tttttctgac
      421 taaccggtaa attgtcagga taagtaattt ccggtcgtgc cgcttcacgc agcaggactt
      481 tccctgccgc ctggtcaatc tctttcgcca tctcctggaa aatggcctgt tgtgcatcag
      541 gatttttaac cttcttaaca ccgtgcagac gccgagaaaa acgcagtctg tcgcgcagca
      601 tcagcgaatc cagccgctgc tgcaaggccg taaaggtcaa tttttgttgt tctgtcataa
      661 cgttagtggg cagtagtctg cctgattaat tttcttttaa atgatagata tagagtacca
      721 catcgcggct ttctatgcgt tgttcaataa attcgaacat aggcttcgat ttattgcgct
      781 atctctgtgg caggattgtg aataaagtgt cagcaagcaa cggggcatcg cccattcaaa
      841 catctataag gaaacaccat gagcaaggta ttagttctta aatccagcat cctggcagag
      901 tactctcagt ctaatcagtt gtccgattat tttgttgaac aatggcgcga aaagcactcc
      961 gctgatgaaa tcaccgttcg cgacctggct gcaaatccga ttccggtact ggatggcgaa
     1021 ctggttggtg cactgcgtcc gagcgatgcg ccgctgactc cgcgtcagca ggaagctctg
     1081 gcgctttccg atgagctgat tgccgagctg aaagcccacg acgttatcgt tattgcggca
     1141 ccgatgtata acttcaacat ctcaactcag ttgaaaaact attttgacct ggttgcacgc
     1201 gcaggcgtta ctttccgcta taccgagaac ggtccggaag gtctggtaac gggtaaaaaa
     1261 gccatcgtta ttaccagccg cggtgggatc cacaaagatg gaccaacgga cctggtgacg
     1321 ccgtatctgt ccacgttcct cgtctttatc ggcattaccg atgtgaaatt tgtcttcgcc
     1381 gaagggatcg catacggtcc ggaaatggca gcgaaagcgc agtctgacgc gaaagcggcc
     1441 atcgacagca ttgttgctgc ataaccggac acgatcttga tgtggactgt agttcagtcc
     1501 acatctcaat ccacttacct gttttcccat aatttcagca tcgctttgtg atcttcctta
     1561 agcacaatat gcgaacgtct ggctcgaata taactaatcg cttcatcaac ggttttacag
     1621 tgtccgtaac ataacaacca tgccgccact accagcgcac tgcgcgataa tcccaacgcg
     1681 caatggacca gaacgctgcc ttgctcttcg cgtaatgttt ccagcatcgc cacggcctgt
     1741 cggagctccc cctcttccgg aaccaccaga tccagcatcg gtacacagaa atagagtcga
     1801 tcttttgtcg ctcgtccccg agggaattca aaggtgacgt ccagaaccgc attctgtgcc
     1861 ggaatatgtc gtggaaacgc ccctaaataa acaccagcag taattttgct caccggctcc
     1921 aggcgacgac aaaaccaacg catagacagc cacatcccga tgcgccaggg caatgtcagc
     1981 cagtaaacgg cgggcgggag tttcccctga ctatctttcc ctgttgttat cgcgccaagc
     2041 ccaccgtacc cacggccaat aatgagtagc gataatactg gccaacataa ccagactgac
     2101 caccataact gaatcatcac cattagctcc atcaacacaa tgcacgagcc cgcgcctacg
     2161 acatatggca gtgctatttt gattcgacgt tgatcaggtt tctgataatt ccaacgacga
     2221 tcgacgggca ccatccagtc aatcaacata cctaccgcca gccctgtgat gacatcaata
     2281 aaatgatgct gccaggtcgt cagagtagaa atagcgatga gtaaaaacca tccgccacag
     2341 actttacgcc acctctcagc cagatgctga cgaaagtgac gccaaagtag ccagcagaga
     2401 ataatatgca gcgaaggaga ctggttataa ggcagatcaa acagttcaag ttgcgaaaat
     2461 agccatcccg tcaccccact cacttcagga cggataaaac taaacttcag cggatagagc
     2521 aaaaaaccgc agcaggccat taccgttgcc agaataagcc ggtggacaag tcggcgctgt
     2581 tcgaatgtcg tgctacaaac gaacagcgaa aatccatata aaagatccag actccagtaa
     2641 ggaacaatag tccagggaag aaaagggatc gccgtttccc aaccgaatac ctgactgggg
     2701 atatcatggc tgttaaggtc ctgaaccgcg gtgaactgat taagagatcc ataggtgaag
     2761 aagaaaaacg gggccagcaa caataaccag ccagcgcctt gtagcaatac gttccgacgt
     2821 tcagttatca cgacgcaccg ccatcgaaac cgtaaaaata ccccactcat caatccgttg
     2881 tgtgcattta tcaaatccgg catcacgcac gagtgaatcc atctcccctt gcgaacgcac
     2941 gcgcattacc cacggtttac catctttatg actggttaac accccggcaa tcatctccag
     3001 ttgagggtgc cacggctgcc cggtgtagat gagaatgccg cccggttcga tggcattggc
     3061 aagacctgcg agtgagtttt ttacctgctc attttcggga aaaagctcat acaggccaga
     3121 gacaatcgcc agcgtaggcc gcggagttaa cgcgctgagt tcctccgggt taaacgcatc
     3181 gccctgttca aaacgcaccc gcccagacat tccccgttgg gcaatcatcg cttgcccctg
     3241 tgcaacattt aactcactgt aatcacgtaa caaaatatcg cttacggcag gctcgtttgc
     3301 cagcgcatcc agtacatagc gcccatgccc tgcggcaatg tcaaccacgc ggacggctaa
     3361 acctttggcg tggagatcgg caacggcttg tttaatcagt atttgtaaat gggttttacg
     3421 ctggcgaata ccgcgccagc caacactgtt caggtagatt ttgtcaatta agcgcccgaa
     3481 tgcgttactg ccttgcggtt gattttgata gacataatcc agggaactgc ctgaatcaaa
     3541 gccggtgctc attccgagat gcaggcccgc agagtgcgcc ccgaacaatt tcatcgcctt
     3601 tcgcataaag cgatacgcca aatcaaccgg cgataatggc acgggtccac ctgaaaggag
     3661 ccgccagcga tccgctgatg gtccagtgcg gtcttcatgc tgataatcaa atttttgtga
     3721 cttgttagca tataaacgac taataaagct ttgcattttt tcaaatgcct gcgccctgtt
     3781 ctcttcaccc aacgtgtcgt gataaaagcc tggcagcaga tgcagctctt tcagagggct
     3841 acgtaatctc tgataaaaat caatctgcgg ttggcgatgc accacatagt catcgcctga
     3901 tatcagaagt tgcgtgggga gcgtaatcgc cgccgcatct ctaataatac gttcagacgt
     3961 tttgtagaga tcgagcaaga tattaacggc aatcgcccgt gtgatcagcg gatcattatt
     4021 gaaactcgcc acccgttgcc gatcgtgggt caaatagcgt cctttcacat aggaattaat
     4081 aaaaaacaga ccacgcagac gatgccataa cgccagcgca ggacgtgcca gcggtacata
     4141 caatttaacc ttaaaggccg gagaagccag caccagcccg cgaattgcag gcgcataatc
     4201 atgaacccat gtggcaacca gcactgcgcc gacgctttgc gcgatcacta ccacctcatc
     4261 cagtccgacc tggctatcgc tggcagcaaa acgaacaaat tcatcgacat cctgcactga
     4321 acgcgcaaga gatgggctat aaccacgcgg ccccgaagtt tgtccatgcc ctcgggcatc
     4381 ccaggcataa aaaacagtat ctggcatcgc cagttcatca acgagatgtt gtagacgacc
     4441 agaatgttca tgaccgcgat gaaataagac gatgaccttt ttagccccgg gctgtaaagc
     4501 gggccagtgc cgataaaaca acgctgtatt atcactgctg gtaaaaaaat gttccccatg
     4561 gatgcgtgaa ttttccattt tctctatttt ccttcagtaa cagcagtagc gaataaaata
     4621 aaaaaatacc ggagcggtga aaattaatga gtcaatcctg tcaaggagtc cgccatgtcc
     4681 tggtaatagt ttcccactat ctttaacacc aatatctcgt ttgatggctg acatcacgac
     4741 atcgccgcaa aaaccactaa taccaattaa caaacccgct aataatgcct gtaatgtatt
     4801 tagcggtgtc agcaacggtc caataatcag tgacgcaatc atggtggtga ttacgccccc
     4861 caccagacct tccagcgttt ttcccgggct gactttgggg accacttttc ttctgccgca
     4921 ggatttcccc cataaatact gtgcaatatc gtttgactcg gttaatgcca aaagaaataa
     4981 caccagtaac gccccgccct ggatatttgt ggttggcaac attaataacc aggcggcatg
     5041 actcaaggcg aaaacggtca ttatccagcc ccaaaaaatg gcgctcacgg tatgcagaaa
     5101 accggagggg tcccccataa aaacttgccc ggtggctaat attagaaacc cagccaaagg
     5161 tataaataat agatacagct caaagcaatt aaaaccgata aacaaatagt taagaggaat
     5221 aacccaatat aaccaccgcg gaaaatgtac agatataagc gtacaatatt ctttcaatgc
     5281 cagaaaactt atcagcgcaa aaaacgtcaa cgtcatccag cgtggacctg aaatcaccag
     5341 tgaaaaacaa atgacgatgc cccaccaggt acgaatacgt aatgtaactt catcctcttt
     5401 tctctccggt aaccgccata gcagaaaatg gtttatcagc gttgctaaaa ttaacaacgc
     5461 gaaaagtgtt gccagagatt tttccagcat tatctttcag cgctcataag aagcacactc
     5521 cgacaacgat taatcgcagt ccagagaagc agaattgaag caatgctcca taaaagatta
     5581 ttccactgca tccactgtgg ataaatggca acggccagac cccacagacc aaatattaac
     5641 gcgcgatcgc ttttgccaaa tggaccggca taactgcgaa caccgttaat cgtctgcgcg
     5701 agtaaaccgc aaaactcggt caatatggtg caaaataaca tgagtatcac gagtgatgcg
     5761 ttactttccg gtaaaaataa aaatggtaaa tagagcgcaa tatcggaaat aacatcacca
     5821 gtctcattca aaattgcccc aagccgtgtt tgctgattgc actcacgcgc caacatgcca
     5881 tccagcgcat tgagcgccat acggataaaa agcacgatgg gcaatagcat aaagaggatg
     5941 ggttgtgcca ccaacatcaa cagcaatccg gtaagaagag aaagcgccag tgcagcaaga
     6001 gtgatgtgat tcgctgtaac gtggtgcttg taaagccaaa acatcgtcgg ccttaacagc
     6061 gactgaaaga gcggttttat ctggtagagc gtcattatca atccttgata attcggtttg
     6121 cgctatggtg catcaattcc cctgacggag atatcgcaac acgccgcttc attaaaaatt
     6181 cagaatatat caaggtattg gggactcact gtcaggaata attatcccca attaatcaaa
     6241 gggttaagaa atattacgtt gtggatcaat acaactcatc aagttttctc atcgcttgtt
     6301 cccgtattgc gcgcgtctta ccgttaagcg ccagctggcg gactaaatcc agttcagggt
     6361 taacaagctc taacgccagc aaaggatcac gtttcagcct cgcctttaat acatcaactg
     6421 gaaaacgggg attattcatc gccacaatcc accactcggg atcaatttct gccgccagta
     6481 ctgcaggcgg tgtgtggcgg ttgttaagtg cgccgaactt gaaaaatgtg agattgcttt
     6541 tcgccagtgt actccaccat atacgatccg gttgttttgc cagtacatcc agttgtgcta
     6601 acgcatcggg gtcgagtgca taatagacgc gtaatgcttc atcaacctgc gcacgggagc
     6661 tgttttcctg cctcagaaaa aacttcgccc aaatatcatt gcgtggcgta ttttgctgta
     6721 attccttgag gacaacagcg ggtaaattat cccaactaac taatgcactc tctaaaaagt
     6781 cccagtcttc caaacgtatc agggcggtgg catttaatag catctgatca tcaacttcac
     6841 cgtggctgat aagctcagac aaaatcatat cagcgctggt ttcatacgca gctgaatctg
     6901 ctttcggatt atttttccga tgcgataaag cacgttgcca aattttttgt ttcatcgacg
     6961 tgctcagata ctgatttttc gccagattac tccacccctc aggatcgttg aaacgtgtca
     7021 ataaccgttg tagagcagcg tcagagtgaa ttacctgcat atcccgacag caaaagtagg
     7081 caggggattt tttctccatc cttttaacaa aattaaactg taagttatct ggcaatgcta
     7141 aaaataacac acgtattaga tcatcgtcat tctcgaattt ttggtataaa taggctgcaa
     7201 ctttgtcact ctcttgtggc gtcatccgca ataattgacc atggtgtttc atctctgcca
     7261 gcttgaacgc taagcttgtg acaacattgt ttgatgaatc gttcgccaac tgcattgcct
     7321 gattgtaggt gagttgtcct ccagcaccga cctgacgaat agaggaatca tcactgcgga
     7381 tatagccatc aataatggat tcaggcaata accacgcatc tttataaagc tcctgaatga
     7441 tcatatccca atctcttttc gccagagtca ttacgatatc cagagggaga ttttcacgta
     7501 gcgcaagatt caacggaccg acaccatcag gtagacgact cattcgcgtc attatttcga
     7561 cagagagcgg ttcacggtcg agcaacccca gaacaacagg ctggtaaccc aattgctgat
     7621 cggtcaactc cagaatctca ttgtcaggca tatatctcgc catactttca atcgtttgtt
     7681 catcattgct attaataagt tgtttgcgta ggttagctga aattccttta agccttggga
     7741 gagttcgcat tatggcaaga tcaaatgtcg caatatctcg tccttcaaat gcagcaacgt
     7801 tgccttcatc cagcatttgc aattcctgca ctaactcagg agaaggctta gggcgaaaca
     7861 gcgcttcttt tttcatctga ttacagacat tccctgccat gcgaatattg cttcgcttaa
     7921 aaatttcttt cagagcatct tcatcattta ttagacccca ataatatgca tcaggattta
     7981 ttatcatcag cgcttttttg acattttcag tgtcattttt gtcactcgca cggaccatga
     8041 attcactatt tgtcgaagac gcaacaacag gtgcaattat gtcattgata aatattccac
     8101 gcgcattaat actcccctta atcgaagacg gattatcgta atatgagtcg ataaatacca
     8161 gtccacttgc atttatcgaa ccggtaatta acagtggtga atccgtaact atattcgttg
     8221 ccgtcacgtt accgagaata acccatggat tagaaagaga gaaagtggaa atattacctt
     8281 tgactgtcaa attaccaaga acaatcaatt tttgattact ctctatatta ccttctacca
     8341 ctaaatcgcc acgatgcact ttccagtccg atgcagataa accgaagggg acaagattca
     8401 cgctactgtt gatagcttgt tcaacttgtt gtttatcttc agcggtagcc gcagaaaaaa
     8461 atggttcaat atcaactgta tttgcaacac atcgaagttc cgcctgtact ggaagactca
     8521 gcataaacag gggaagtaaa cctatccagc cgcaaatcct ggacttaaac atatgcactc
     8581 catgccatta aaaaagaaac aaaagggaaa tgcatctttc ccttttgtat attcgttatt
     8641 ctcgcgaaat accatccaac gtagacaaca cttcctcaga aagatgcaat ttttcagcag
     8701 ccatattctc ccgtaaatgc gcaacagaag acgtccctgg gatcagcaaa atattcggtg
     8761 aacgttgtaa cagccacgcc agcgccacct gcatgggtgt tgcacccagg ctcgcagcaa
     8821 catccgacag cgtagaggat tgcagcggtg taaagccccc gagcgggaag aacggcacgt
     8881 aggcaatgcc gtcacgagcc aaagcatcaa tcattgcgtc atcagcacga tgagcaatgt
     8941 tgtattcgtt ctgcacacag acgatttcgg caatcttgcg cgcctctgca acctgcgtcg
     9001 gtgtgacgtt gcttaggcca atatgtttta ccaggccttg ttgttgcatc tctgccagca
     9061 cggtcaggct ggcttcaatc gatccttccg ctgggccatg accatccccc atcataatgc
     9121 gcaagttaac cacatccagt acgtccagcc cgagattacg tagattatcg tgcaccgctt
     9181 tttgcagctc tgccggagaa aatgcgggca accaggatgc gtcctctcca cgccgcgcac
     9241 caattttagt gacaattgtc aggtcgtcag agtaaggata aagcgcttcg cggataatct
     9301 gattggtgac gtgcggacca taaaagtcgc tggtatcaat gtgattgacg cccaatgcca
     9361 gcgcctcgcg cagcacggtt atagcgacgt ggcgatctct tgggggacca aaaaccccag
     9421 gacctgccag ttgcatcgcg ccataaccaa gacggttaac ggattttgta ccgagagtaa
     9481 atgtattgct gctcatcgca ccctgccctg taataaaacg gattgagaaa aatcagtata
     9541 gaaacgaaaa gagataaata atgcgaaggc gtaaacaaga ttactggatg agttgctcta
     9601 ttgtcctgaa agacaccaaa acaaaacgcg taaagcgccg cattcggcaa taaaaaaacc
     9661 gcaacctgta tcagcgttgc ggtttaggta tcacatgcta aaaattagaa cgttcagtat
     9721 ctggacgtta ctgtttcggt cgcagattct catcaaatac caggcgtttc ctgccaggtt
     9781 caacttcccg caatggttcg acttgatgta acgtctgctt acagcgtgtc accagcacct
     9841 gatactcatg cgtaccctgc tttttccatg ccagctcctg ctcactaagc tcacgaatca
     9901 gtttcgccgg gctgccgaca atcaggtaat tagcaggcat ttctgctttg gctttcacaa
     9961 atgcggatgc gccaacaatg ctgttctcgc caatcaccgc accatccatc actaccgcgt
    10021 tcattcccac taatgcattg cggcggataa tgcagccgtg aaggatagcg ctatgaccaa
    10081 tatgcccatc ttcttcaacg acagtatcct gctcgggaaa accgtgcata acgcaattat
    10141 cctgaatgtt cgcgccatct ttcaccacga tacgaccaaa atcgccacgc aggctggcat
    10201 ttggcccaac gtaaacaccc ttgccgagaa taacatcgcc gatcaatacc gctgtcggat
    10261 ggacaaaact ctcttctggc acaaccggag tcagaccgtc tatctgataa attggcataa
    10321 cgcctcctgt tcaatattca aaccgccaaa acgttgaaaa tacaggctac ccggcgaagg
    10381 cagttcaccg accgaggttt cacctttttc actaacgaac gctaaagcag caggcgctac
    10441 gcgctgataa atgttgatac acagctgacg cgccgtatgc cctgcccagt gagccggaag
    10501 caactcctcc ggcaacaaag ggtctttaag gacgacacgg cgataaaaat ggatcagtaa
    10561 aagctgaata tgaaatgcac gctccggggt taactcgtct gctgcctctt ttaacagctg
    10621 caccaacggg cggaatgact gaataaaggt ttcgtacatg gcattttgtt cagttaaatg
    10681 ccagcactct tctactctgg cacgcagtgc tgcacgagaa agcgccagtg gtatttgcgc
    10741 ttcaaaacaa atcacgttat ccgccacacc cgcttcatgc aaaagtgtct gtacatcggc
    10801 cagtttttgc gacggcgatg ccatcaggct gggtgccagt gcgccaaaac cttgccagat
    10861 caactgcttt ttgacatcag ccagcgttga tttatctaaa ccttccgaaa gcaacaggag
    10921 ccatttacca tcccatgcag gttgctctgc gcgataaatt ttactttctg cccgtcgcgt
    10981 caggcgcaag cctttatcac tgaggctata gaaactgcgt cgcccgatgc gggaaacatc
    11041 cagccagcct tctttattaa gacgaaacaa agcggtgcgc acgaatcgct caccaaatcc
    11101 cagcccttcc agtaaagcag ccagactacc cagccagatt tcaccaccac gatgggaaag
    11161 cgaatcacca tacagagagg agatcaaaga tgtgccactg acaggaacag cgtttacagc
    11221 atgttggata aaagtatcaa gtttactcat tcgattcagt ctgttgttat tgttgtgatt
    11281 tatgaatcat agcacaggtc gtatcgactt ctggccctgg cgctttaccc cacccgggcc
    11341 agacgacacg tcaggcacca acaatattgc gcagatcaaa cacccgacac gccttgcctt
    11401 ctgaacgcgg gatactgcca cagttaacga tcatcacatc ggtagagatc cccaccatcg
    11461 acttaatccg atggcgcagc tggtggcaca cctggcaacg ctgctcatgc gtcaatgtca
    11521 ggctgctttc tttcaactcc actttcacag aaagtgaatc aagatgcccc cggcggttca
    11581 cctccagttg gtaatggggc gataaatgtt cgaatttgac aatctcttct tccagttgtg
    11641 acgggaagac atttacaccg cgaatgatca acatgtcatc gctgcgtccg ctgatgcgat
    11701 ccatgcggcg catagtccgc gccgttcctg gtaacagtcg ggttaaatca cgcgtgcggt
    11761 aacgaatgac cggcaatgct tctttggtca gcgtggtgaa taacagttcg ccatgttcac
    11821 catcggcaag cggtgtgccg tcatgaggat taacaatttc aggatagaaa tgatcttccc
    11881 aaatggttgg gccgtcggta gtttccagac actccatcgc cacccccggc cccatcactt
    11941 ctgacaggcc ataaatatcc agtgcggtga tccccagacg ccgttcaatc tctttacgca
    12001 tggcctgtgt ccacggctca gcaccaaaca ctccaacccg cagcgagcaa ccgctggcat
    12061 caccgcccaa ctgccgctcc agctcttcaa tcaggttaag gcaataagat ggcgtaacca
    12121 tgatcatatc tggttgaaaa tcgcggatca gttgcgcttg tttttccgtc tgcccaccag
    12181 acatcgggat caccgtagcg cccagacgtt cggcaccata gtgcgcgccc agcccaccag
    12241 taaacaggcc gtaaccatag gcaacatgaa ttttgtcttt gggcgtgcca cctgccgcac
    12301 gcaaggaacg cgctacaata ttggcccagt tatcaatatc gttttgcgta tagccgacaa
    12361 ctgtcggttt acctgtggtt cccgaagaag catgaatgcg caccacttgt tccatcggca
    12421 cggcaaaggt gtcgaaggga tagttgtcgc gcagatcctg tttggtggta cagggaaatt
    12481 tacgcagatc tgaaagttcc ctgaaatcat caggatgtac gcctgctgcg tcaaatttgc
    12541 gccgatacat cgggacattt tcatacgcgt gtttgagcgt ccatttcaga cgttgtgttt
    12601 gcaacgcctg taactcatca acagacgcgg tttcaatcgg gtcaagcttt gtattggtta
    12661 tcattgtagg gtactcgcag gttgatatgc tcaaacacgc tccagaatca tggcgatgcc
    12721 ctgaccgaca ccgatgcaca tggtgcacaa tgcgtaacga ccgttacgcc gatgcagctc
    12781 atggctggca gccagtgcca ggcgggcacc actcattccc aacggatggc ctaaggcgat
    12841 agcgcctccg ttggggttaa catgtggggc atcatcaggc agccccaatt cgcgtagtac
    12901 acccaacgcc tgggccgcga acgcttcgtt cagttcaatc acgtccatat cgtgaatact
    12961 cagccccgcg cgttccagca cccggcgagt tgcaggcacc ggaccaagcc ccatcaggcg
    13021 cggttccacc ccggcggttg ccatggctac gattcgcgcc cgcggtgtca gtccttgcgc
    13081 tgctgccatc tgttcactgg caataatcaa cgcagcggct ccgtcattca ccccggaggc
    13141 attgcctgcg gtaatcaccc cattggcacg aaatggtgct tttaacccac gtaactgttc
    13201 cagcgtcgtt tccgggcgca gatgctcatc atgttgtatt tctgttacaa cgcctttctt
    13261 gtttttcaac acaaccggaa caatctcctc agccagaatg cctgaggatt gcgcttttgc
    13321 cgtacgttgc tgactgcgta gcgcaaaact atcttgatct tctcgcgata tttttaacag
    13381 ttcagctaca ttctctgccg tttccggcat gctgtcagtt ccaaattgtt gagccatgag
    13441 cgggttcaca aatcgccagc caatagtggt atcaaacatc tcagcctgac gagaaaatgc
    13501 actggttgcc ttgcccataa caaacggtgc ccgagacatt gactccacgc caccagcgat
    13561 cagcaaatcg ccatcgcccg ctttaatcgc ccgtgcggcg aaccccagtg cgtccagccc
    13621 ggaaccacac aagcggttaa tggttgtgcc ggaaacactc tgcggcagcc ccgccagtaa
    13681 cgtcgccatc cgggctacgt tacggttgtc ttctcccgcc tgattagcac agccgaggat
    13741 cacatcatcg atacactccg catcgagacg cgggtttcgc accagcagct cccgcaaagg
    13801 gatagcagcc agatcgtcag cccgaacacc tgataatgcc ccaccatagc ggccaattgg
    13861 cgtacgaatt ccgtcacaga taaaggcttc acgcatcagg cttctcctgt aatggtgccg
    13921 ccgatgcggt gagatttacc gcgaaacagc gcaaccgttt tttgttgttg gttaacaatt
    13981 tcgatgtcgt aaacaccggt ttgcttgccc tgatgacgta cctgcgcagt agcagttaag
    14041 gtgtctccgg caaagcctgg acgtaaaaaa tcaatcgtgc aggcagaagc gacggctgcc
    14101 agcccctggc tattgcaggc gtaggcaaag gcagtatcag ccagtgaaaa tagctgcccg
    14161 ccgtggcaac tttgatgacc gttaagcatt tgtgcagtga cggtcatggt cactacagca
    14221 aagccttcat ccattgagat aatgtcgatg ccaagcgctt tggcgcaggc atcgttctca
    14281 tacattgcat gggcattttg ccaggcctta tgactcataa ccgctctcca gaagcgcccg
    14341 ttggcgcagc aatgaacatg ggcgatagcg ttcttcgcca tagtgatgct gtagattttc
    14401 aaggagcctt aatattcgct gccagccaag ttgcgctccc caggcaagtg ggccatatgg
    14461 ataattcacc ccaagacgca tggcggtatc gatatcctgt tcagtggcca cgcctttttg
    14521 aagcgcatca agggcttcat tgatgatcat tgctaccgtt cgccaaatca gcattcctgg
    14581 gtaatctgca atttgcagca ctgttttgcc ctgctgttgc aggtaataaa tggccttgcg
    14641 ggtcgctgag tccgggttca ccgctgccgc agcaatggtc accaccttgc ccgccatttt
    14701 gtcgaccact accaccgggc gtgccagtcg tattgccagc gcctgtgccg tctcgccttg
    14761 tgtctcaatc aataaaacat cgtcaatttc cgtgacaccg tcacttttct tttctacttt
    14821 cattgggcta aaactgtcgc ttaccgcttc caggccaaca actgcctcac gttccgcgcg
    14881 ccagtcgtac acgcccagcc cacttttctt gcccaaccgt ccaccaatca ccagttcctg
    14941 ttgcaccagc gaaggtaaaa aacgacgctc ctgccagaaa gcgttaaaca ccgaacaggt
    15001 gacagcaaaa ttgacgtcct gaccaatcag atcggttaat tccagcggcc ccatcgggaa
    15061 accagcgcca tcgcgaagtg cagcgtcaat cacttctggt gcagcaacct gctcttccag
    15121 tgcccgccag gcctcggaat aataaggacg cgcaacacgg ttaacgataa atccgggggt
    15181 cgaatgacag cgcactggct gcttacccca actcaatgtc agttcacaca actgctcgac
    15241 aacttccgct gccgttgcca gcccactgac cacctccacc aacttcatca ccggtgccgg
    15301 gttaaaaaaa tgcagccccg caacacgttc aggatttttt acctccgcag caatcgcggt
    15361 tatagagatt gacgaagtgt tagtggtcaa tagcgtttgt ggcgggcaaa cttccgccag
    15421 ttgtgcaaag agcgcttttt taacttccag acgttcagac gccgcttcaa taaccagatt
    15481 cgctgctgcc agcgcgtgaa tatcggtcac cgggatcagg cgtttcaatg tgcgttcaca
    15541 ggtttcagcg gtcagttttc cccgcgtcac gcgtgaattt agccgcgcgt gtatcccgtc
    15601 gattgcgcgg gtcagcgctt cagcagaaat gtcatacagt aaaacctgat gtccatgact
    15661 ggcggcaact tcagcaatgc ctgcccccat ggtgccgctc ccaatcactg ccacagtttg
    15721 cacatttatc atcatgctat ttccccgtga actgcggtga gcgtttagcc aggaacgcac
    15781 tgacaccttc acgataatct gcgctccgtc cggcaagtcg ctgatagtca cgttccagat
    15841 ccagttgcgt atcgagcgta ttggtttcag cgctatttat cgcttgcttg ataagtccaa
    15901 gaccaaatgt cggttgtgtc gccagatgcc gtgccagctg ttgcgcggta tctgccagcg
    15961 tttcatcatc aacaacctgc cagatcatcc cccattcgtg cgcctgttca gcactcagtt
    16021 gattccccag cagtgccagc cccatggcgc gcgctcgtcc ggcaacgcgt ggcagtaacc
    16081 aggttccacc gcaatcgggt attaagccta acttactaaa cgccatgacg aattttgctg
    16141 aacgggcagc aataacgatg tcgcacccca gtgccagtgt tgcgcctgcc cctgccgcca
    16201 cgccattgac tgcacagatc accggttttg gcagttttgc caggcgacgt accagtgggt
    16261 tatagaaacg ttcaactgac atccctaaat ccggtgcggg gcctgtggga tcaacgttac
    16321 gatcgttaag atcctgacca gcacaaaatc cgcgcccggc acctgtaagt aacaggcaac
    16381 ggatagtgtc gtcgcgctcg acctgtttca ggcactctgc cagttgtgcg tgcatctcat
    16441 cattaaaact gttcaggcgt tccgggcggt tgagcgttag tgtcatcacg cccttttcta
    16501 catgactgag gatgaattcc atcattagcg tcctttaaag tcgggcgtgc gtttttgtaa
    16561 gaaagcggag atgccttcat gacgatcttc tgttgccgcc agcaaggtga ataactgtcg
    16621 ctcctgggca agtccggctt gcaaagccac ttcctgcgac tggcgcagcg cttgctttgc
    16681 cgcttgtaag gccagcggcg agtgacgtgc cattttcgat gccagctgta aggcgtattc
    16741 gagggttaaa tcgctgggga agacgtcgct aaccagcccg gcctgctgtg cttgctgggc
    16801 ggtgatactt tctccgctca gcaccatttt gctggctaac gatttaccga cactacggat
    16861 taaacgttgc gttccgcctg cgccaggcat gatgccgaga gtgatttccg gcaacccaaa
    16921 acgcgcgttc tctccggcaa ccaccacatc gcacaacaat gccagttcgc aacccgcccc
    16981 aagcgcgtaa ccattgacgg ctgcgataag tggtttatta aaggcctgca atcgcgccca
    17041 tagctgcgga cgtgtatcgt ttaaggtggc cgcgagatct ttttctgcca tttcgttgag
    17101 atcggcccca gcggcaaaaa agcgtgcatt accggtaatc acacagaccg aaatgctggt
    17161 atcggtagcc gcagcttcca gttcatttac cagttgcatc agcagggcat tatttagcgc
    17221 attacgtgcg gcgggacggt taagggtcag caacaatact cgttgctgac ggctgacgat
    17281 cagttcgctc atgccatccc cttcgcgtca aagtcaacca ccacatcact ggtcagcggc
    17341 agtgcctggc aactcaacac atagcctgca gccagttcat ccggttccag actgtaattg
    17401 gtttccatcg ccactttgcc acgcagcact ttgcatttgc aggtcgcaca gacgccgcct
    17461 ttgcaggcat agggcagatc agccccctgg cgcaatgccg catcgagaat gctttcatcg
    17521 tcggcattaa gcacgatttc ccgatcccgc ccatcctgac gtacagtcac tttttgtccg
    17581 tcactttgca cgttaacgct acgtttgacg cgcgtgccag gcgtattaaa ccgctccaga
    17641 tgaatggttt tatctggcat tcccagtgct tttaaggcgg cttccgtctc atccatcatc
    17701 gccgccggac cacaaataaa tgcctcatca taaagacgaa aattaatgag cgaggcccca
    17761 agtgactgta atttttcacc gtcaatacgc ccgtgaagca gatcgctgtc gagggtttcc
    17821 tgactgaaaa tgcacaacaa ctgtaaacgc tgaggatatt tgtctttcag gtctgccagt
    17881 gcctggcgaa acatcatgct ctggctggta cggttaccgt agatcagggt gaactgactt
    17941 tcaggctcgg tttgtaaagt ggcggcgata atcgccagca ttggcgtaat acctgatcct
    18001 gctgcaatag ccagatagcg cccctggcgt tcggcctgcg gctgatagcc gaaatggccc
    18061 tgcggcacca tgacttccag cgtcatcccc tgacggatgt gatcgcgggc atagcgggag
    18121 aaacgtccgc cttcaatggc ttttaccgcc acactaattt cgccaggcag gtagctgcgg
    18181 caaatggagt aacaacggcg taattcttca ccatcaaggc tggcttttaa ggtcaagtgt
    18241 tgaccggggc gaaagcgata cgcctcctgc aaaggctggg gcaccgcaaa ggtaatggtc
    18301 accgcatcac gggtttccgg ctccactttt gccaccgtta aggaatgaaa cgttgtcatg
    18361 gcatcctcaa atacatttga aataatcgaa aggttcgcgg caactatcgc agcgatacaa
    18421 tgctttgcag gccgtggaac caaattcact gataagtgtg gtatggacgc tggcacagcg
    18481 cgggcaacat acttctggcg gcaaatgggc atggcaactg tgtccggcgg gcgggctaat
    18541 gccatactgt cgcagacgct cacgggcatc gggggtcatc cagtcggtgg tccatgccgg
    18601 gtcaagttgc agcacaacct gaacgggggt aaacccgtgg gttgacattg cctcacgtat
    18661 cgcgccaatc aaatgttccg ttgccggaca accggaatat gtcggcgtaa agccgatcac
    18721 ccatccttct cccatctgtg tcacattacg caccatgcct aaatcagtaa tggtcagcac
    18781 cgggatctcc gggtccggga tctggctgag tagcgcccat atctcatgaa cctgtggcgg
    18841 tgcaatggtg gccagacgtt gcatacccat ctcctctgtt accattgctg accgggcaag
    18901 acacgctgga gatactgcat ttctgccagc atgggtccaa ggtgttcggt atgcagtcct
    18961 tttttaccgc cagtgcgata cgcctgctct tgcggtacgt tcaatgtggc ttcgttgatc
    19021 ccagcaaaaa cttcggcttc ccacgctgcg cgtaaagtgc gtggatcaac cgcaataccc
    19081 tcttcactca gtgcaatatc aatctcgtcg gcatcgaaca gttcggcggt aaaacgccac
    19141 aacttgttga tggcctgctg catcttttgc cctgatacgt cagtaccatt gcccagtcgc
    19201 tccagccagc cacgactaaa acgcaggtga taacgcgctt ctttaattgc tttggcagaa
    19261 atcgccgcca gttgcggatc acggctctcc atcagacggg taaagagcgc cacatgccat
    19321 gcatcgatga aatactggcg tgcaatggtg tcggcaaaat tgccgtttgg ctgttcaacc
    19381 aacaataagt tgctgaactg gcgctcgtct cgggtgaagg ccagggtatc ttcatcgcct
    19441 tctcccgcta attcagcggc atacgataag aagttgcgtg cctggcctaa taaatcgagt
    19501 ccaatatttg ccagtgcgag atcgatttcc agttccggtg cgtgaccgca ccattccccc
    19561 aaccgctggg agagcaccag acagttatcg cccaggcgca aggtgtaagc cgttaactga
    19621 ttcatttccg acctcacatg tgctcaatgc catcagggat ggtgtaaaac gttggatggc
    19681 gatagacctt gctttcagcc ggatcaaaaa attcaccgcg ttcttccggt tgcgaggcaa
    19741 caatttcact cgccttcacc acccaaattg aacatccttc gctacgacgg gtgtaagcat
    19801 cacgggcatt ttccagtgcc atccgctcat cggcagcatg taaactgcca acatggcggt
    19861 gtgataagcc ctgtttgcca cgcacgaaaa cttcgtataa cggccagtaa acattactca
    19921 ttttgaatct ccttatgcga ccttacgggc aagttgtttt tgtgcatggg ccagcgctgc
    19981 ttcccgtacc caggtacctt cttcccaggc tttacgtttg gcgtcgagcc tctcctgatt
    20041 acaaattccg cgaccgttaa ttacttcgtt aaactcctgc cagtcgatct caccaaagcg
    20101 gtagtgaccg ctttcagtgt caaaatgcag atccgggtca ggaacggtca taccgagcat
    20161 ttcaacctgt ggaacggtgt tgtccacgaa acgctggcgg agttcgtcgt tggtgaaacg
    20221 tttgattttc caggcgagac ttctggcgct gtttggcgag ttatcatcgt ttggcccgaa
    20281 catcattaag gctggccacc agaaacggtt aatggcgtct tgcaacatct gcttttgagc
    20341 ttcgctgccc tgcgccagtg ccatgcaggc ttcaaacccc tgacgctggt gaaaactctc
    20401 ttctttgcag attttcacca ttgctctggc atacgggcca taagaagtgc ggcataacgc
    20461 cacctgattg acgatcgctg cgccatccac cagccagccg ataacaccga tatcagccca
    20521 actcaatgtc ggataattaa agatggagga atatttcatc cgtccgtcga gcattttttg
    20581 gtagatgtct tctctggcgc agcccagtgt ttcggcggcg ctatagagat acagaccatg
    20641 accggcttca tcctgcactt tggccaacag aatggcttta cgccgcaagg ttggtgcgcg
    20701 agtgatccag ttaccttcag gcaacatacc aacaatctcg gagtgcgcat gctgcccaat
    20761 ctggcggatc agtgtctttc ggtaagcatc gggcatccag tcctgcggct cgatagccgt
    20821 ttcctgggct atccgttgct caaagcgttc ttcttgggtc acgtcatcac ctttacgatt
    20881 ccagttttcg tgaattaatt aaaaacacga atcacgttgt tttatgaaag ttacacaatg
    20941 gttaactaaa atcacaaatt ttgtatgttt gaactgtgac ggatttcgca ccctattttt
    21001 ataactcatt atatatcagt gcattaatgt ttttagtggt tgatatacag atcgcatagt
    21061 taacatttcg ttaaaatacg ccttgctttt tatgattcgt gatttaacta ttagcaacag
    21121 aaatgtgaaa catctggaga gtagcgatgc agcagttagc cagtttctta tccggtacct
    21181 ggcagtctgg ccggggccgt agccgtttga ttcatcacgc tattagcggc gaggcgttat
    21241 gggaagtgac cagtgaaggt cttgatatgg cggctgcccg cctgtttgcc attgaaaaag
    21301 gtgccccagc ccttcgcgct atgaccttta tcgaacgtgc ggcgatgctt aaagcggtcg
    21361 ctaaacatct gctgagtgaa aaagagcgct tctatgctct ttctgcgcaa acaggcgcaa
    21421 cgcgggcaga cagttgggtt gatattgaag gtggcattgg gacgttattt acttacgcca
    21481 gcctcggtag ccgggagctg cctgacgata cgctgtggcc ggaagatgaa ttgatcccct
    21541 tatcgaaaga aggtggattt gccgcgcgcc atgtactgac ctcaaagtca ggcgtggcag
    21601 tgcatattaa cgcctttaac ttcccctgct ggggaatgct ggaaaagctg gcaccaacgt
    21661 ggctgggcgg aatgccagcc atcatcaaac cagctaccgc gacggcccaa ctgactcagg
    21721 cgatggtgaa atcaattgtc gatagtggtc ttgttcccga aggcgcaatt agtctgatct
    21781 gcggtagtgc tggcgacttg ttggatcatc tggacagcca ggatgtggtg actttcacgg
    21841 ggtcagcggc gaccggacag atgctgcgag ttcagccaaa tatcgtcgcc aaatctatcc
    21901 ccttcactat ggaagctgat tccctgaact gctgcgtact gggcgaagat gtcaccccgg
    21961 atcaaccgga gtttgcgctg tttattcgtg aagttgtgcg tgagatgacc acaaaagccg
    22021 ggcaaaaatg tacggcaatc cggcggatta ttgtgccgca ggcattggtt aatgctgtca
    22081 gtgatgctct ggttgcgcga ttacagaaag tcgtggtcgg tgatcctgct caggaaggcg
    22141 tgaaaatggg cgcactggta aatgctgagc agcgtgccga tgtgcaggaa aaagtgaaca
    22201 tattgctggc tgcaggatgc gagattcgcc tcggtggtca ggcggattta tctgctgcgg
    22261 gtgccttctt cccgccaacc ttattgtact gtccgcagcc ggatgaagca ccggcggtac
    22321 atgcaacaga agcctttggc cctgtcgcaa cgctgatgcc agcacaaaac cagcgacatg
    22381 ctctgcaact ggcttgtgca ggcggcggta gccttgcggg aacgctggtg acggctgatc
    22441 cgcaaattgc gcgtcagttt attgcagacg cggcacgtac gcatgggcga attcagatcc
    22501 tcaatgaaga gtcggcaaaa gaatccaccg ggcatggctc cccactgcca caactggtac
    22561 atggtgggcc tggtcgcgca ggaggcggtg aagaattagg tggtttacga gcggtgaaac
    22621 attacatgca gcgaaccgct gttcagggta gtccgacgat gcttgccgct atcagtaaac
    22681 agtgggtgcg cggtgcgaaa gtcgaagaag atcgtattca tccgttccgc aaatattttg
    22741 aggagctgca accaggcgac agcctgttga ctccccgccg cacaatgaca gaggccgata
    22801 ttgttaactt tgcttgcctc agcggcgatc atttctatgc acatatggat aaaattgctg
    22861 ctgccgaatc tatttttggt gagcgtgtgg tacatggata ttttgtgctt tctgcggctg
    22921 cgggtctgtt tgtcgatgcc ggtgtcggtc cggtcatagc taactacggg ctggaaaact
    22981 tgcgttttat cgaacccgta aaaccaggcg ataccatcca ggtgcgtctc acctgtaagc
    23041 gcaagacgct gaaaaaacag cgtagcgcag aagaaaaacc aacaggtgtg gtggaatggg
    23101 cggtagaggt attcaatcag catcaaaccc cggtggcgct gtattcaatt ctgacgctgg
    23161 tggccaggca gcacggtgat tttgtcgatt aatcggtgaa tgaagggcaa cggcgaatag
    23221 ttgccctttt atttcactaa attttgtgac gttgtcacat tatgcatgat gtgtacatct
    23281 attttcaggg catccactgt atgaaaagct ggcacacctg ccaaacaacc tggcaggtgc
    23341 aggcaatccc ctttgcatca gtactgataa tgtgaacctg actaaaccgc ccacagagcg
    23401 cggttgctaa caagaacaca acatctgacg aggttaataa tgggaagccc ctctctgtat
    23461 tctgcccgta aaacaaccct ggcgttggca gtcgccttaa gtttcgcctg gcaagcgccg
    23521 gtatttgccc acggtggtga agcgcatatg gtgccaatgg ataaaacgct taaagaattt
    23581 ggtgccgatg tgcagtggga cgactacgcc cagctcttta ccctgattaa agatggcgcg
    23641 tacgtgaaag tgaagcctgg tgcgcaaaca gcaattgtta atggtcagcc tctggcactg
    23701 caagtaccgg tagtgatgaa agacaataaa gcctgggttt ctgacacctt tattaacgat
    23761 gttttccagt ccgggctgga tcaaaccttt caggtagaaa agcgccctca ccctcttaat
    23821 gcgctaactg cggacgaaat taaacaggcc gttgaaattg ttaaagcttc cgcggacttc
    23881 aaacccaata cccgttttac tgagatctcc ctgctaccgc cagataaaga agctgtctgg
    23941 gcgtttgcgc tggaaaacaa accggttgac cagccgcgca aagccgacgt cattatgctc
    24001 gacggcaaac atatcatcga agcggtggtg gatctgcaaa acaacaaact gctctcctgg
    24061 caacccatta aagacgccca cggtatggtg ttgctggatg atttcgc
//
LOCUS       DAMZET010000056        23909 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.056, whole genome shotgun sequence.
ACCESSION   DAMZET010000056 DAMZET010000000
VERSION     DAMZET010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.056"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..532
                     /gene="rplC"
                     /locus_tag="POB34_002282"
     CDS             <1..532
                     /gene="rplC"
                     /locus_tag="POB34_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDI5919219.1"
                     /translation="VTQVKDLANDGYRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGR
                     GLWEFRLAEGEEFTVGQSISVELFADVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHG
                     NSLSHRVPGSIGQNQTPGKVFKGKKMAGQMGNERVTVQSLDVVRVDAERNLLLVKGAV
                     PGATGSDLIVKPAVKA"
     gene            543..1148
                     /gene="rplD"
                     /locus_tag="POB34_002283"
     CDS             543..1148
                     /gene="rplD"
                     /locus_tag="POB34_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDI5919220.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            1145..1447
                     /gene="rplW"
                     /locus_tag="POB34_002284"
     CDS             1145..1447
                     /gene="rplW"
                     /locus_tag="POB34_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDI5919221.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            1465..2286
                     /gene="rplB"
                     /locus_tag="POB34_002285"
     CDS             1465..2286
                     /gene="rplB"
                     /locus_tag="POB34_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDI5919222.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            2303..2581
                     /gene="rpsS"
                     /locus_tag="POB34_002286"
     CDS             2303..2581
                     /gene="rpsS"
                     /locus_tag="POB34_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDI5919223.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            2596..2928
                     /gene="rplV"
                     /locus_tag="POB34_002287"
     CDS             2596..2928
                     /gene="rplV"
                     /locus_tag="POB34_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDI5919224.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            2946..3647
                     /gene="rpsC"
                     /locus_tag="POB34_002288"
     CDS             2946..3647
                     /gene="rpsC"
                     /locus_tag="POB34_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDI5919225.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            3660..4070
                     /gene="rplP"
                     /locus_tag="POB34_002289"
     CDS             3660..4070
                     /gene="rplP"
                     /locus_tag="POB34_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDI5919226.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            4070..4261
                     /gene="rpmC"
                     /locus_tag="POB34_002290"
     CDS             4070..4261
                     /gene="rpmC"
                     /locus_tag="POB34_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDI5919227.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            4261..4515
                     /gene="rpsQ"
                     /locus_tag="POB34_002291"
     CDS             4261..4515
                     /gene="rpsQ"
                     /locus_tag="POB34_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDI5919228.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            4680..5051
                     /gene="rplN"
                     /locus_tag="POB34_002292"
     CDS             4680..5051
                     /gene="rplN"
                     /locus_tag="POB34_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDI5919229.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            5062..5376
                     /gene="rplX"
                     /locus_tag="POB34_002293"
     CDS             5062..5376
                     /gene="rplX"
                     /locus_tag="POB34_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDI5919230.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            5391..5930
                     /gene="rplE"
                     /locus_tag="POB34_002294"
     CDS             5391..5930
                     /gene="rplE"
                     /locus_tag="POB34_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDI5919231.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            5945..6250
                     /gene="rpsN"
                     /locus_tag="POB34_002295"
     CDS             5945..6250
                     /gene="rpsN"
                     /locus_tag="POB34_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDI5919232.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            6284..6676
                     /gene="rpsH"
                     /locus_tag="POB34_002296"
     CDS             6284..6676
                     /gene="rpsH"
                     /locus_tag="POB34_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDI5919233.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            6689..7222
                     /gene="rplF"
                     /locus_tag="POB34_002297"
     CDS             6689..7222
                     /gene="rplF"
                     /locus_tag="POB34_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDI5919234.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            7232..7585
                     /gene="rplR"
                     /locus_tag="POB34_002298"
     CDS             7232..7585
                     /gene="rplR"
                     /locus_tag="POB34_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDI5919235.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            7600..8103
                     /gene="rpsE"
                     /locus_tag="POB34_002299"
     CDS             7600..8103
                     /gene="rpsE"
                     /locus_tag="POB34_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDI5919236.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            8107..8286
                     /gene="rpmD"
                     /locus_tag="POB34_002300"
     CDS             8107..8286
                     /gene="rpmD"
                     /locus_tag="POB34_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDI5919237.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            8290..8724
                     /gene="rplO"
                     /locus_tag="POB34_002301"
     CDS             8290..8724
                     /gene="rplO"
                     /locus_tag="POB34_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDI5919238.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            8732..10063
                     /gene="secY"
                     /locus_tag="POB34_002302"
     CDS             8732..10063
                     /gene="secY"
                     /locus_tag="POB34_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDI5919239.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            10095..10211
                     /gene="rpmJ"
                     /locus_tag="POB34_002303"
     CDS             10095..10211
                     /gene="rpmJ"
                     /locus_tag="POB34_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDI5919240.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            10358..10714
                     /gene="rpsM"
                     /locus_tag="POB34_002304"
     CDS             10358..10714
                     /gene="rpsM"
                     /locus_tag="POB34_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDI5919241.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            10731..11120
                     /gene="rpsK"
                     /locus_tag="POB34_002305"
     CDS             10731..11120
                     /gene="rpsK"
                     /locus_tag="POB34_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDI5919242.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            11154..11774
                     /gene="rpsD"
                     /locus_tag="POB34_002306"
     CDS             11154..11774
                     /gene="rpsD"
                     /locus_tag="POB34_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDI5919243.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            11800..12789
                     /gene="rpoA"
                     /locus_tag="POB34_002307"
     CDS             11800..12789
                     /gene="rpoA"
                     /locus_tag="POB34_002307"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDI5919244.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            12830..13213
                     /gene="rplQ"
                     /locus_tag="POB34_002308"
     CDS             12830..13213
                     /gene="rplQ"
                     /locus_tag="POB34_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDI5919245.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            13320..13688
                     /locus_tag="POB34_002309"
     CDS             13320..13688
                     /locus_tag="POB34_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HDI5919246.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            13699..14124
                     /gene="zntR"
                     /locus_tag="POB34_002310"
     CDS             13699..14124
                     /gene="zntR"
                     /locus_tag="POB34_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HDI5919247.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            14180..14398
                     /gene="arfA"
                     /locus_tag="POB34_002311"
     CDS             14180..14398
                     /gene="arfA"
                     /locus_tag="POB34_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HDI5919248.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(14395..14805)
                     /gene="mscL"
                     /locus_tag="POB34_002312"
     CDS             complement(14395..14805)
                     /gene="mscL"
                     /locus_tag="POB34_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDI5919249.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(14935..16311)
                     /gene="trkA"
                     /locus_tag="POB34_002313"
     CDS             complement(14935..16311)
                     /gene="trkA"
                     /locus_tag="POB34_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HDI5919250.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(16333..17622)
                     /gene="rsmB"
                     /locus_tag="POB34_002314"
     CDS             complement(16333..17622)
                     /gene="rsmB"
                     /locus_tag="POB34_002314"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDI5919251.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGTEEGDGFFYAKLIKK"
     gene            complement(17668..18615)
                     /gene="fmt"
                     /locus_tag="POB34_002315"
     CDS             complement(17668..18615)
                     /gene="fmt"
                     /locus_tag="POB34_002315"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDI5919252.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(18630..19139)
                     /gene="def"
                     /locus_tag="POB34_002316"
     CDS             complement(18630..19139)
                     /gene="def"
                     /locus_tag="POB34_002316"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDI5919253.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            19269..20393
                     /gene="dprA"
                     /locus_tag="POB34_002317"
     CDS             19269..20393
                     /gene="dprA"
                     /locus_tag="POB34_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDI5919254.1"
                     /translation="MVDTDIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            20365..20838
                     /locus_tag="POB34_002318"
     CDS             20365..20838
                     /locus_tag="POB34_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HDI5919255.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            20867..21409
                     /locus_tag="POB34_002319"
     CDS             20867..21409
                     /locus_tag="POB34_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HDI5919256.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCLACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            21414..21986
                     /gene="tsaC"
                     /locus_tag="POB34_002320"
     CDS             21414..21986
                     /gene="tsaC"
                     /locus_tag="POB34_002320"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HDI5919257.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            21991..22809
                     /gene="aroE"
                     /locus_tag="POB34_002321"
     CDS             21991..22809
                     /gene="aroE"
                     /locus_tag="POB34_002321"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDI5919258.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLQGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALSMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKCNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            22806..23063
                     /locus_tag="POB34_002322"
     CDS             22806..23063
                     /locus_tag="POB34_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HDI5919259.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(23039..23593)
                     /locus_tag="POB34_002323"
     CDS             complement(23039..23593)
                     /locus_tag="POB34_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDI5919260.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6069 a         5690 c         6253 g         5897 t
ORIGIN      
        1 cgttactcag gttaaagacc tggctaacga tggctaccgt gctattcagg tgaccaccgg
       61 tgctaaaaaa gctaaccgtg tgaccaagcc tgaagctggc cacttcgcta aagctggcgt
      121 agaagctggc cgtggtctgt gggaattccg cctggctgaa ggcgaagagt tcactgtagg
      181 tcagagcatt agcgttgaac tgtttgctga cgttaaaaaa gttgacgtaa ctggcacctc
      241 taaaggtaaa ggtttcgcag gtaccgttaa gcgctggaac ttccgtaccc aggacgctac
      301 tcacggtaac tccttgtctc accgcgttcc gggttctatc ggtcagaacc agactccggg
      361 caaagtgttc aaaggcaaga aaatggcagg tcagatgggt aacgaacgtg taaccgttca
      421 gagccttgac gtagtacgcg ttgacgctga gcgcaacctg ctgctggtta aaggtgctgt
      481 cccgggtgca accggtagcg acctgatcgt taaaccagct gtgaaggcgt aaggagatag
      541 caatggaatt agtattgaaa gacgcgcaga gcgcgctgac tgtttccgaa actaccttcg
      601 gtcgtgattt caacgaagcg ctggttcacc aggttgttgt tgcttatgca gctggtgctc
      661 gtcagggtac tcgtgctcag aagactcgtg ctgaagtaac tggttccggt aaaaaaccgt
      721 ggcgccagaa aggcaccggc cgtgcgcgtt ctggttctat caagagcccg atctggcgtt
      781 ctggtggcgt gacctttgct gctcgtccgc aggaccacag tcaaaaagtt aacaagaaga
      841 tgtaccgcgg cgcgctgaaa agcatcctgt ccgaactggt acgtcaggat cgtctgatcg
      901 ttgtcgagaa gttctctgta gaagcgccga aaactaagct gctggcacag aaactgaaag
      961 acatggctct ggaagatgtg ctgatcatca ccggtgagct ggacgaaaac ctgttcctgg
     1021 ctgcgcgcaa cctgcacaag gttgacgtac gcgatgcaac tggtatcgac ccggttagcc
     1081 tgatcgcctt cgacaaagtc gtaatgactg ctgatgctgt taagcaagtt gaggagatgc
     1141 tggcatgatt cgtgaagaac gtctgctgaa ggtgctgcgt gcaccgcacg tttctgaaaa
     1201 agcgtctact gcgatggaaa aatccaacac catcgtactc aaagttgcta aagacgcgac
     1261 caaagcagaa atcaaagctg ctgtgcagaa actgtttgaa gtcgaagtcg aagtcgttaa
     1321 caccctggta gttaaaggga aagttaaacg tcacggacag cgtatcggtc gtcgtagcga
     1381 ctggaaaaaa gcttacgtca ccctgaaaga aggccagaat ctggacttcg ttggcggcgc
     1441 tgagtaagtc ggaggagtaa tacaatggca gttgttaaat gtaaaccgac atctccgggt
     1501 cgtcgccacg tagttaaagt ggttaaccct gagctgcaca agggcaaacc ttttgctccg
     1561 ttgctggaaa aaaacagcaa atccggtggt cgtaacaaca atggccgtat caccactcgt
     1621 catatcggtg gtggccacaa gcaggcttac cgtattgttg acttcaaacg caacaaagac
     1681 ggtatcccgg cagttgttga acgtcttgag tacgatccga accgttccgc gaacatcgcg
     1741 ctggttctgt acaaagacgg tgaacgccgt tacatcctgg cccctaaagg cctgaaagct
     1801 ggcgaccaga ttcagtctgg cgttgatgct gcaatcaaac caggtaacac cctgccgatg
     1861 cgcaacatcc cggttggttc tactgttcat aacgtagaaa tgaaaccagg taaaggcggt
     1921 cagctggcac gttccgctgg tacttacgtt cagatcgttg ctcgtgatgg tgcttatgtc
     1981 accctgcgtc tgcgttctgg tgaaatgcgt aaagtagaag cagactgccg tgcaactctg
     2041 ggcgaagttg gcaatgctga gcatatgctg cgcgttctgg gtaaagcagg tgctgcacgc
     2101 tggcgtggtg ttcgtccgac cgttcgcggt accgcgatga acccggtaga ccacccacat
     2161 ggtggtggtg aaggtcgtaa ctttggtaag cacccggtaa ctccgtgggg cgttcagacc
     2221 aaaggtaaga agacccgcag caacaagcgt actgataaat tcatcgtacg tcgccgtagc
     2281 aaataatttt agaggataag ccatgccacg ttctctcaag aaaggtcctt ttattgacct
     2341 gcacttgctg aagaaggtag agaaagcggt ggaaagcgga gacaagaagc ccctgcgcac
     2401 ttggtcccgt cgttcaacga tctttcctaa catgatcggt ttgaccatcg ctgtccataa
     2461 tggtcgtcag cacgttccgg tatttgtaac cgacgaaatg gtcggtcaca aactgggtga
     2521 attcgcaccg actcgtactt atcgcggcca cgctgctgat aaaaaagcga agaagaaata
     2581 aggtaggagg aagagatgga aactatcgct aaacatcgcc atgctcgttc ttctgctcag
     2641 aaggttcgcc ttgttgctga cctgattcgc ggtaagaaag tgtcgcaggc tctggatatt
     2701 ttgacctaca ccaacaagaa agcggctgta ctggtcaaga aagttctgga atctgccatt
     2761 gctaacgctg aacacaacga tggcgctgac attgacgatc tgaaagttac gaaaattttc
     2821 gtagacgaag gcccgagcat gaagcgcatt atgccgcgtg caaaaggtcg tgcagatcgc
     2881 atcctgaagc gcaccagcca catcactgtg gttgtgtccg atcgctgaga ctctggagac
     2941 tagcaatggg tcagaaagta catcctaatg gtattcgcct gggtattgta aaaccatgga
     3001 actctacctg gtttgcgaac accaaagaat tcgctgacaa cctggacagc gattttaaag
     3061 tacgtcagta cctgactaag gaactggcta aagcgtccgt atctcgtatc gttatcgagc
     3121 gtccggctaa gagcatccgt gtaaccattc acactgctcg cccgggtatc gttatcggta
     3181 aaaaaggtga agacgtagaa aaactgcgta aggtcgtagc ggacatcgct ggcgttcctg
     3241 cacagatcaa catcgccgaa gttcgtaagc ctgaactgga cgcaaaactg gttgctgaca
     3301 gcatcacttc tcagctggaa cgtcgcgtta tgttccgtcg tgctatgaag cgtgctgtac
     3361 agaacgcaat gcgtctgggc gctaaaggta ttaaagttga agttagcggc cgtctgggcg
     3421 gcgcggaaat cgcacgtacc gaatggtacc gcgaaggtcg cgtaccgctg cacactctgc
     3481 gtgctgacat cgactacaac acctctgaag cgcacaccac ttacggtgta atcggcgtta
     3541 aagtgtggat cttcaaaggc gagatcctgg gtggtatggc tgctgttgaa caaccggaaa
     3601 aaccggctgc tcagcctaaa aagcagcagc gtaaaggccg taaataagga gcgtcgctga
     3661 tgttacaacc aaagcgtaca aaattccgta aaatgcacaa aggccgtaac cgcggtctgg
     3721 cgcagggtac ggatgttagc ttcggcagct tcggtctgaa agctgttggc cgtggtcgtc
     3781 tgactgcccg tcagatcgaa gcagcacgtc gtgctatgac ccgtgcagtt aagcgtcaag
     3841 gtaagatctg gatccgtgtg ttcccggaca aaccgatcac tgaaaagccg ctggcagtgc
     3901 gtatgggtaa aggtaaaggt aacgtggagt attgggttgc cttgattcag ccgggtaaag
     3961 tcctgtatga aatggacggt gttccggaag agctggcccg tgaagcattc aagctggcag
     4021 cagcgaaact gccgattaaa accacctttg taactaagac ggtgatgtaa tgaaagcaaa
     4081 agagctgcgt gagaagagcg ttgaagagct gaacaccgag ctgctgaacc tgctgcgtga
     4141 gcagttcaac ctgcgtatgc aggctgcaag tggccagctg caacagtctc acctgttgaa
     4201 gcaagtgcgt cgcgatgtcg cacgcgttaa gactttactg aacgagaagg cgggtgcgta
     4261 atgaccgata aaatccgtac tctgcaaggt cgcgttgtta gcgacaaaat ggagaaatcc
     4321 attgttgttg ctatcgaacg ttttgtgaaa cacccgatct acggtaaatt catcaagcgt
     4381 acgaccaaac tgcacgtaca tgacgagaac aacgaatgcg gtatcggtga cgtggttgaa
     4441 atccgcgaat gccgtccgct gtccaagact aaatcctgga cgctggttcg cgttgtagag
     4501 aaagcggttc tgtaatacag tacactctct cgatacgaat aaacggctca gaaatgagcc
     4561 gtttattttt tctacccata tccttgaagc ggtgttataa tgccgcgccc tcgatatggg
     4621 gatttttaac gacctgattt tcgggtctca gtagtagttg acattagcgg agcactaaaa
     4681 tgatccaaga acagactatg ctgaacgtcg ccgacaactc cggtgcacgt cgcgtaatgt
     4741 gtatcaaggt tctgggtggc tcgcaccgtc gctacgcagg cgtaggcgac atcatcaaga
     4801 tcaccatcaa agaagcaatt ccgcgtggta aggtcaaaaa aggtgatgtg ctgaaggcgg
     4861 tagtggtgcg caccaagaag ggtgttcgtc gcccggacgg ttctgtcatt cgcttcgatg
     4921 gtaatgcttg tgttcttctg aacaacaaca gcgagcagcc tatcggtacg cgtatttttg
     4981 ggccggtaac tcgtgagctt cgtagtgaga agttcatgaa aattatctct ctggcaccag
     5041 aagtactcta aggagcgaat catggcagcg aaaatccgtc gtgatgacga agttatcgtg
     5101 ttaaccggta aagataaagg taaacgcggt aaagttaaga atgtcctgtc ttccggcaag
     5161 gtcattgttg aaggtatcaa cctggttaag aaacatcaga agccggttcc ggccctgaac
     5221 caaccgggtg gcatcgttga aaaagaagcc gctattcagg tttccaacgt agcaatcttc
     5281 aatgcggcaa ccggcaaggc tgaccgtgta ggctttagat tcgaagacgg caaaaaagtc
     5341 cgtttcttca agtctaacag cgaaactatc aagtaatttg gagtagtacg atggcgaaac
     5401 tgcatgatta ctacaaagac gaagtagtta aaaaactcat gactgagttt aactacaatt
     5461 ctgtcatgca agtccctcgg gtcgagaaga tcaccctgaa catgggtgtt ggtgaagcga
     5521 tcgctgacaa aaaactgctg gataacgcag cagcagacct ggcagcaatc tccggtcaaa
     5581 aaccgctgat caccaaagca cgcaaatctg ttgcaggctt caaaatccgt cagggctatc
     5641 cgatcggctg taaagtaact ctgcgtggcg aacgcatgtg ggagttcttt gagcgcctga
     5701 tcactattgc tgtacctcgt atccgtgact tccgtggcct gtccgctaag tctttcgacg
     5761 gtcgtggtaa ctacagcatg ggtgtccgtg agcagatcat cttcccagaa atcgactacg
     5821 ataaagtcga ccgcgttcgt ggtttggata ttaccattac cactactgcg aaatctgacg
     5881 aagaaggccg cgctctgctg gctgcctttg acttcccgtt ccgcaagtaa ggtagggtta
     5941 ctaaatggct aagcaatcaa tgaaagcacg cgaagtaaaa cgcgtagctt tagctgataa
     6001 atacttcgcg aaacgcgctg aactgaaagc gatcatctct gatgtgaacg cttccgacga
     6061 agatcgttgg aacgctgttc tcaagctgca gactctgccg cgtgattcca gcccgtctcg
     6121 tcagcgtaac cgctgccgtc aaacaggtcg tccgcatggt ttcctgcgga agttcgggtt
     6181 gagccgtatt aaggtccgtg aagccgctat gcgcggtgaa atcccgggtc tgaaaaaggc
     6241 tagctggtaa ttgtcaccaa ttgaatcacg ggagtaaaga cagatgagca tgcaagatcc
     6301 gatcgcggat atgctgaccc gtatccgtaa cggtcaggcc gcgaacaaag ctgcggtcac
     6361 catgccttcc tccaagctga aagtggcaat cgccaacgtg ctgaaggaag aaggttttat
     6421 tgaagatttt aaagttgaag gcgacaccaa gcctgaactg gaacttactc tgaagtattt
     6481 ccagggcaaa gctgttgtag aaagcattca gcgtgtcagc cgcccaggtc tgcgcatcta
     6541 taaacgtaaa gatgagctgc cgaaagttat ggcgggtctg ggtatcgcag ttgtttctac
     6601 ctctaaaggt gttatgactg atcgtgcagc gcgccaggct ggtcttggtg gcgaaattat
     6661 ctgctacgta gcctaatcgg aggaaaaaat gtctcgtgtt gctaaagcac cggtcgttgt
     6721 tcctgccggc gttgacgtaa aaatcaacgg tcaggttatt acgatcaaag gtaaaaacgg
     6781 cgagctgact cgtactctca acgatgctgt tgaagttaaa catgcagata ataccctgac
     6841 cttcggtccg cgtgatggtt acgcagacgg ttgggcacag gctggtaccg cgcgtgccct
     6901 gctgaactca atggttatcg gtgttaccga aggcttcact aagaagctgc agctggttgg
     6961 tgtaggttac cgtgcagcgg ttaaaggcaa tgtgattaac ctgtctctgg gtttctctca
     7021 tcctgttgac catcagctgc ctgcgggtat cactgctgaa tgtccgactc agactgaaat
     7081 cgtgctgaaa ggcgctgata agcaggtgat cggccaggtt gcagcggatc tgcgcgccta
     7141 ccgtcgtcct gagccttata aaggcaaggg tgttcgttac gccgacgaag tcgtgcgtac
     7201 caaagaggct aagaagaagt aaggtaacac tatggataag aaatctgctc gtatccgtcg
     7261 tgcgacccgc gcacgccgca agctccagga gctgggcgca actcgcctgg tggtacatcg
     7321 taccccgcgt cacatttacg cacaggtaat tgcaccgaac ggttctgaag ttctggtagc
     7381 tgcttctact gtagaaaaag ctatcgctga acaactgaag tacaccggta acaaagacgc
     7441 ggctgcagct gtgggtaaag ctgtcgctga acgcgctctg gaaaaaggca tcaaagatgt
     7501 atcctttgac cgttccgggt tccaatatca tggtcgtgtc caggcactgg cagatgctgc
     7561 ccgtgaagct ggccttcagt tctaaggtag aggtgtaaga tggctcacat cgaaaaacaa
     7621 gctggcgaac tgcaggaaaa gctgatcgcg gtaaaccgcg tatctaaaac cgttaaaggt
     7681 ggtcgtattt tctccttcac agctctgact gtagttggcg atggtaacgg tcgcgttggt
     7741 tttggttacg gtaaagcgcg tgaagttcca gcagcgatcc agaaagcgat ggaaaaagcc
     7801 cgtcgcaata tgattaacgt cgcgctgaat aacggcactc tgcaacaccc tgttaaaggt
     7861 gttcacacgg gttctcgcgt attcatgcag ccggcttccg aaggtaccgg tatcatcgcc
     7921 ggtggtgcaa tgcgcgccgt tctggaagtc gctggggttc ataacgttct ggctaaagca
     7981 tatggttcca ccaacccgat caacgtggtt cgtgcaacta ttgatggcct ggaaaatatg
     8041 aattctccag aaatggtcgc tgccaagcgt ggtaaatccg ttgaagaaat tctggggaaa
     8101 taaaccatgg caaagactat taaaattact caaacccgca gtgcaatcgg tcgtctgccg
     8161 aaacacaagg caacgctgct tggcctgggt ctgcgtcgta ttggtcacac cgtagagcgc
     8221 gaggatactc ctgctattcg cggtatgatc aacgcggttt ccttcatggt taaagttgag
     8281 gagtaagaga tgcgtttaaa tactctgtct ccggccgaag gctccaaaaa ggcgggtaaa
     8341 cgcctgggtc gtggtatcgg ttctggcctc ggtaaaaccg gtggtcgtgg tcacaaaggt
     8401 cagaaatctc gttctggcgg tggcgtacgt cgcggtttcg agggtggtca gatgcctctg
     8461 taccgtcgtc tgccgaaatt cggcttcact tctcgtaaag cagcgattac agccgaagtt
     8521 cgtctgtctg acctggctaa agtagaaggc ggtgttgtag acctgaacac gctgaaagcg
     8581 gctaacatta tcggtatcca gatcgagttc gcgaaagtga tcctggctgg cgaagtaacg
     8641 actccggtaa ctgttcgtgg cctgcgtgtt actaaaggcg ctcgtgctgc tatcgaagct
     8701 gctggcggta aaatcgagga ataagtagca gatggctaaa caaccgggat tagattttca
     8761 aagtgccaaa ggtggcttag gcgagctgaa acgcagactg ctgtttgtta tcggtgcgct
     8821 gattgtgttc cgtattggct cttttattcc gatccctggt attgatgccg ctgtacttgc
     8881 caaactgctt gagcaacagc gaggcaccat cattgagatg tttaacatgt tctctggtgg
     8941 tgctctcagc cgtgcttcta tctttgctct ggggatcatg ccgtatattt cggcgtcgat
     9001 cattatccag ctgctgacgg tggttcaccc aacgttggca gaaattaaga aagaagggga
     9061 gtctggtcgt cgtaagatca gccagtacac ccgttacggt actctggtgc tggcaatatt
     9121 ccagtcgatc ggtattgcta ccggtctgcc gaatatgcct ggtatgcaag gcctggtgat
     9181 taacccaggc tttgcattct acttcaccgc tgttgtaagt ctggtcacag gaacgatgtt
     9241 cctgatgtgg ttgggcgaac agattactga acgaggtatc ggcaacggta tttcaatcat
     9301 tatcttcgcc ggtattgtcg cgggactccc gccagccatt gcccatacta tcgagcaagc
     9361 gcgtcaaggc gacctgcact tcctcgtgtt gctgttggtt gcagtattag tatttgcagt
     9421 gacgttcttt gttgtatttg ttgagcgtgg tcaacgccgc attgtggtaa actacgcgaa
     9481 acgtcagcaa ggtcgtcgtg tctatgctgc acagagcaca catttaccgc tgaaagtgaa
     9541 tatggcgggg gtaatcccgg caatcttcgc ttccagtatt attctgttcc cggcgaccat
     9601 cgcgtcatgg ttcgggggcg gtactggttg gaactggctg acaacaattt cgctgtattt
     9661 gcagcctggg caaccgcttt atgtgttact ctatgcgtct gcaatcatct tcttctgttt
     9721 cttctacacg gcgttggttt tcaacccgcg tgaaacagca gataacctga agaagtccgg
     9781 tgcatttgta ccaggaattc gtccgggaga gcaaacggcg aagtatatcg ataaagtaat
     9841 gacccgcctg accctggttg gtgcgctgta tattaccttt atctgcctga tcccggagtt
     9901 catgcgtgat gcaatgaaag taccgttcta cttcggtggg acctcactgc ttatcgttgt
     9961 tgtcgtgatt atggacttta tggctcaagt gcaaactctg atgatgtcca gtcagtatga
    10021 gtctgcattg aagaaggcga acctgaaagg ctacggccga taattggtcg cccgagaagt
    10081 tacggagagt aaaaatgaaa gttcgtgctt ccgtcaagaa attatgccgt aactgcaaaa
    10141 tcgttaagcg tgatggtgtc atccgtgtga tttgcagtgc cgagccgaag cataaacagc
    10201 gccaaggctg attttttcgc atatttttct tgcaaagttg ggttgagctg gctagattag
    10261 ccagccaatc ttttgtatgt ctgtgcgttt ccatttgagt atcctgaaaa cgggcttttc
    10321 agcatggaac gtacatatta aatagtagga gtgcatagtg gcccgtatag caggcattaa
    10381 cattcctgat cataagcatg ccgtaatcgc attaacttcg atttatggcg tcggcaagac
    10441 ccgttctaaa gccatcctgg ctgcagcggg tatcgctgaa gatgttaaga tcagtgagct
    10501 gtctgaagga caaatcgaca cgctgcgtga cgaagttgcc aaatttgtcg ttgaaggtga
    10561 tctgcgccgt gaaatcagca tgagcatcaa gcgcctgatg gatcttggtt gctatcgcgg
    10621 tttgcgtcat cgtcgtggtc tcccggttcg cggtcagcgt accaagacca acgcacgtac
    10681 ccgtaagggt ccgcgcaaac cgatcaagaa ataatcgggg tgattgaata atggcaaagg
    10741 caccaattcg tgcacgtaaa cgtgtaagaa aacaagtctc tgacggcgtg gctcatatcc
    10801 atgcttcttt caacaacacc atcgtgacta tcactgatcg tcagggtaac gcgttgggtt
    10861 gggcaacagc cggtggttcc ggtttccgtg gttctcgcaa atccactccg tttgcagctc
    10921 aggttgcagc agagcgttgc gctgacgccg tgaaagaata cggcatcaag aatctggaag
    10981 ttatggttaa aggtccgggt ccaggccgcg aatctactat tcgtgctctg aacgccgcag
    11041 gtttccgcat cactaacatt actgatgtga ctccgatccc tcataacggt tgtcgtccgc
    11101 cgaaaaaacg tcgcgtataa cgcctcgttt tccaggtttg ttggagaaag aaaatggcaa
    11161 gatatttggg tcctaagctc aagctgagcc gtcgtgaggg caccgactta ttccttaagt
    11221 ctggcgttcg cgcgatcgat accaagtgta aaattgaaca agctcctggc cagcacggtg
    11281 cgcgtaaacc gcgtctgtct gactatggtg tgcagttgcg tgaaaagcaa aaagttcgcc
    11341 gtatctatgg tgtgctggag cgtcagttcc gtaactacta caaagaagca gcacgtctga
    11401 aaggcaacac cggtgaaaac ctgttggctc tgctggaagg tcgtctggac aacgttgtat
    11461 accgtatggg cttcggtgcc actcgtgcag aagcacgtca gctggttagc cataaagcaa
    11521 ttatggtaaa cggtcgtgtt gttaacatcg cttcttatca ggttagtccg aatgacgttg
    11581 taagcattcg tgagaaagcg aagaagcagt ctcgcgtgaa agccgctctg gagctggctg
    11641 agcagcgtga aaagccaacc tggctggaag ttgatgctgg caagatggaa ggtacgttta
    11701 agcgtaagcc ggagcgttct gatctgtctg cggacattaa cgaacacctg atcgtcgagc
    11761 tttactccaa gtaaagctta gtaccaaaga gaggacacaa tgcagggttc tgtgacagag
    11821 tttctaaaac cgcgcctggt tgatatcgag caagtgagtt cgacgcacgc caaggtgacc
    11881 cttgagcctt tagagcgtgg ctttggccat actctgggta acgcactgcg ccgtattctg
    11941 ctctcatcga tgccgggttg cgcggtgacc gaggttgaga ttgatggtgt actacatgag
    12001 tacagcacca aagaaggcgt tcaggaagat atcctggaaa tcctgctcaa cctgaaaggg
    12061 ctggcggtga gagttcaggg caaagatgaa gttattctta ccttgaataa atctggcatt
    12121 ggccctgtga ctgcagccga tatcacccac gacggtgatg tcgaaatcgt caagccgcag
    12181 cacgtgatct gccacctgac cgatgagaac gcgtctatta gcatgcgtat caaagttcag
    12241 cgcggtcgtg gttatgtgcc ggcttctacc cgaattcatt cggaagaaga tgagcgccca
    12301 atcggccgtc tgctggtcga cgcatgctac agccctgtgg agcgtattgc ctacaatgtt
    12361 gaagcagcgc gtgtagaaca gcgtaccgac ctggacaagc tggtcatcga aatggaaacc
    12421 aacggcacaa tcgatcctga agaggcgatt cgtcgtgcgg caaccattct ggctgaacaa
    12481 ctggaagctt tcgttgactt acgtgatgta cgtcagcctg aagtgaaaga agagaaacca
    12541 gagttcgatc cgatcctgct gcgccctgtt gacgatctgg aattgactgt ccgctctgct
    12601 aactgcctta aagcagaagc tatccactat atcggtgatc tggtacagcg taccgaggtt
    12661 gagctcctta aaacgcctaa ccttggtaaa aaatctctta ctgagattaa agacgtgctg
    12721 gcttcccgtg gactgtctct gggcatgcgc ctggaaaact ggccaccggc aagcatcgct
    12781 gacgagtaac cggatcacag gttaaggttt tactgagaag gataaggtca tgcgccatcg
    12841 taagagtggt cgtcaactga accgcaacag cagccatcgc caggctatgt tccgcaatat
    12901 ggcaggttca ctggttcgtc atgaaatcat caagacgact ctgcctaaag cgaaagagct
    12961 gcgccgcgta gttgagccgc tgattactct tgccaagact gatagcgttg ctaatcgtcg
    13021 tctggcattc gcccgtactc gtgataacga gatcgtggca aaactgttta acgaactggg
    13081 cccgcgtttc gcgagccgtg ccggtggtta cactcgtatt ctgaagtgtg gcttccgtgc
    13141 aggcgacaac gcgccgatgg cttacatcga gctggttgat cgttcagaga aagcagaagc
    13201 tgctgcagag taatctgaag caacgtaaaa aaacccgccc cggcgggttt ttttataccc
    13261 gtagcatccc cacttatcta caatagctgt actctttttg ttcatcccct ggagtattta
    13321 tgtggttact tgaccagtgg gcagagcgtc atatagcaga agcgcaagcg aaaggtgagt
    13381 ttgataacct ggcaggtagc ggcgaaccat tgatactgga tgatgattct cacgtgccac
    13441 cggaattacg tgcggggtat cgcttgctga agaatgccgg ttgcttaccg ccagaacttg
    13501 agcaacggag agaagcaatt cagcttctgg atatcctgag aggtatccgt cacgatgatc
    13561 cgcaatatca agaggttagc cgtcgattgt cattactgga attgaagctg cgacaagctg
    13621 gattgagtac cgatttttta cgcggcgatt atgctgacaa gttgttggac aaaatcaacg
    13681 ataactagtg gagtatgtat gtatcgcatt ggtgagctgg caaaaatggc ggaagtaaca
    13741 cctgacacga ttcgttatta cgaaaaacag cagatgatgg agcatgaagt gcgtactgaa
    13801 ggcgggtttc gcctgtatac cgaaagcgat ctccagcgat tgaaatttat ccgccatgcc
    13861 agacaactag gtttcagtct ggagtcgatc cgcgagttgc tgtcgatccg catcgatcct
    13921 gaacaccata cctgtcagga gtcaaaaggc attgtgcagg aaagattgca ggaagtcgaa
    13981 gcacggatag ccgagttgca gagtatgcag cgttccttgc aacgccttaa cgatgcctgt
    14041 tgtgggaccg cccatagcag tgtttattgt tcgattcttg aagctcttga acaaggggcg
    14101 agtggcgtta agagtggctg ttgatttttt gcactggcag gattacactc gcgccgtaaa
    14161 ataaccaatt ggagttttta tgagccgata tcagcatact aaagggcaga taaaggataa
    14221 tgcgatagaa gcattactac atgatccctt attccgacag cgcgtagaga aaaataagaa
    14281 ggggaaaggc agttacatgc gaaaaggaaa acatggcaat cgggggaact gggaggccag
    14341 tggcaagaaa gtaaatcact tttttaccac tggccttctg ctttcaggcg tttgttaaga
    14401 gcggttattc tgctctttca gcaaatcacg aatttctgtc agtaatactt cttctttagt
    14461 tggtgctggt gcggctgctg gttcttcttt tttccgattc agtttgttga ttagcttaat
    14521 cgccataaag atggcaaagg ccacaatcag aaaatcaaag acgttttgaa tgaagacacc
    14581 gtaatgcatc acaacagcag ggatatcccc ctgcgcatcg cgtagcgtga cagcaaactg
    14641 tttaaagtcg atcccgccaa ttaataagcc cagaggtggc atgatgatat cggcaaccag
    14701 tgaagataca atcttcccga atgccgcacc gataatgaca cccaccgcca aatccaccac
    14761 gttcccgcgc atcgcaaatt cgcgaaattc tttaataatg ctcatgttat tctccctatg
    14821 aagccgacaa ccataagtct aacaaatgtt aagccatttt cctgccagga atgaatttaa
    14881 ttaatccttg ataatcaaag ggaaataata aggcgtcatt agacgcctta ttaattacaa
    14941 gaagaaaggg cttggctgga agagtctttc gacgtcggta ataaactttt tatctgtgag
    15001 gaacataatt acgtgatcgc cttgctcaat gcgcagattg tcattggcaa tcatcacgtc
    15061 gtttccacgt accaccgctc caataatcgt tcctggcggt agcttgattt cgtcaatgac
    15121 tctgccgaca acgcgtgagg tgctttcatc accgtgagca acggcttcaa tagcttctgc
    15181 tacgccgcgg cgcaatgagg aaacaccaac aatatctgct tttcgcacat ggctaagcaa
    15241 cgcagaaata gttgcttgtt gtggtgaaat cgcaatatcg atcacgctcc cctgaaccag
    15301 atccacataa gcgcgacgct ggatcaatac catcaccttt ttcgcaccca tacgtttggc
    15361 aagcatggcg gacatgatat tggcctcgtc atcgttggtg acggcaataa acagatcaac
    15421 ttgatcgata tgttcttcgg ccagcagttc ttgatccgac gcatcaccaa aaaagacaat
    15481 cgtattctgc aacttttccg ccagttcggc agcgcgctgc tgattacgtt cgatgagttt
    15541 aacgctgtaa tctttttcca gacgacgcgc cagccctgca ccgatattac cgccaccaac
    15601 cagcatgatc cgcttatacg gtttttccag tcgctgtaat tcactcatca ccgcacggat
    15661 atgctgtgaa gcggcaataa agaacacttc atcaccagct tccacaatgg tcgaaccttg
    15721 tggacgaatg gggcgatcgt ggcggaaaat tgctgccaca cgagtatcga tatgtggcat
    15781 atgttcgcgc atggtcgaaa gtgcgttacc aatcagcggg ccgccataat aggctttaac
    15841 cacagcgagg ctgactttac cctcagcgaa gttcaccacc tgcaatgcgc cgggatactc
    15901 aatcagtcgg taaatattat cgataaccaa ctgctctggt gcgatcagat gatcaatcgg
    15961 tacagcatct gaatgaaata gcttatcggc atcgcgcacg tagtctggtg agcggatgcg
    16021 agcgatgcga ttaggggtgt tgaaaagtga gtaggctacc tggcaggcaa ccatattggt
    16081 ttcatctgaa ctggttacag caaccagcat atcggcgtcg tcggcacctg cctcccgcaa
    16141 tacgcgtgga tgagagccat gcccctgcac gactcgcagg tcaaatttat cctgcaaggt
    16201 ccgcagacgc tcaccgttgg tatcgacaac agtgatatcg ttgttctcgc caaccaggtt
    16261 ttccgccagt gtgccgccaa cctggccggc acccagaatg ataattttca tcagtcgcga
    16321 cccgttatct catcactttt tgattagctt agcgtaaaag aagccgtcgc cctcttcggt
    16381 accaggcaga ttttgtttac ccggttgctc tggtgttcct gtttcgcaaa gttcggcatc
    16441 agcggtacgt tgcaaaaagg ctttaatctg caggctattc tcttccggta acaccgaaca
    16501 ggtggcatag accagggttc caccggattt taaatgcggc caaatggcgt cgagaatttc
    16561 agactgcaat tgcgcgagtt ccgggatatc gcgatcgcga cgtaaccatt taatatccgg
    16621 atggcgacga atcacaccgg ttgctgaaca aggcgcatct aataaaatgc gatcaaactg
    16681 ttgctcgcca caccattggg aagggtagcg gccatcacct tgtttcacgg tcgctttcat
    16741 accaaggcgt tttaaattgt cgtaaacgcg agagaggcgc tgttcgtcaa tatcaaccgc
    16801 aacaacctgc gcttctggtg ccacctcaag gatatgcgtt gttttaccgc cgggggcggc
    16861 acaaagatcc agaatgtgtt caccgttttg tggcgcaagc caggtcatgc aaccttgtgc
    16921 tgatgcatcc tgaacggtaa cccatccgtc ttcaaaacca ggtagcgcat gaacaggtgc
    16981 aggtgtttcc agccgtacag catcagggta atccgcatgc gggaaacctt tcattcctgc
    17041 ttcatccaac aatgcaagcc agctgtcgcg ggaatgatgc gtacgattaa cgcgcagcca
    17101 cattggcgga cgctggttat tggcttcgac gatggattgc cactgctctg gatacgcttt
    17161 ttgcagacgc ttcagcaacc aggaaggatg cagataacgt gcatcactgg cattaaactc
    17221 ggctaataac tcttcttgct gacgctggaa ctggcgtaat acaccgttta tcaacccttt
    17281 aagttgcgga cgcttaattg cgatagcgcc ttcaaccgtt tcagccagcg cagcatgagg
    17341 tggaatgcgg gtataaagca gttgatacaa accaaccata atcaggtaat gcacggtccg
    17401 ctgtttgccg gtcatcggac gggccattaa cttattaatc agccagtcta actgcgaaag
    17461 cgtacgcagt acgccaaagc acaactcttg aagaagtgct ttgtctttat cggaaacttt
    17521 ttgctggagc ggtggcagaa tgttgcttaa tgattgccct tgctcgacga cttgttcaac
    17581 ggcctgggcc gccatgctac gtaaattacg ttgttttttc ataagtataa aaatgcccgg
    17641 caagaccggg cttagaagag tggactatca gaccagacgg ttgcccggaa caaaccattc
    17701 ccgacgagag ttcaggaggt cttgcgcgct catcgctttc ttacccgcag gttgtaacga
    17761 gagcagattc aggatgccat caccagtcgc aacctgaatg ccttgtttat tggcttcaag
    17821 gatcgttcct ggtgcagcgt tggttgccgt atcaatgacc gacgctttcc agactttaac
    17881 cggttgtcct tcaatttcca gccagctcat tggccatggg ttgaaagcgc gaatgcagcg
    17941 ttcaagctgc gctgccgaaa gcgaccagtc aatacgcgct tcttctttac tcaacttctc
    18001 ggcgtaagtg acaagagttt cgtcctgaac ttctggtttc gccgtgccgt ctgccagttg
    18061 tttcaacgtg gtgataagcc cttgtgggcc aagctctgcc agcttgtcgt acagcgtacc
    18121 actggtatct tctgcagtaa tcgggcagga gagcttatag agcatatcac cggtgtctaa
    18181 accgacatcc atttgcataa tggtcacacc agtttctgca tcacccgccc atagtgagcg
    18241 ttggattggt gcagcaccgc gccagcgtgg cagcagtgaa ccatgaacgt tgatacagcc
    18301 aagacgcggc atctccagca ctgctttagg cagaattaag ccataggcga cgacgaccat
    18361 aacatcagcc tgcagatcgg cgaccagttg ctggttttct tgtggacgca gggaaacagg
    18421 ttgaaaaacg ggcagacctt tttcctcagc cagaacttta accgggctgg gcatcagttt
    18481 tttaccgcgt cctgccggtc ggtctggctg ggtgaacacg ccaacgacgt tatgaccaga
    18541 agacaacagc gcgtcgagat gacgcgctgc aaagtcaggt gtacccgcaa aaataatacg
    18601 tagtgattct gacacgttag ttcttatcct taagcccggg ctttcagacg atccagtttt
    18661 tcaactttct gacgaatacg ttgttgtttc aacggtgaca gataatccat aaacagtttg
    18721 ccgaccaggt gatccatctc atgctgaata cagatggcta acagaccgtc tgcttccagt
    18781 tcaaatggtt taccgtcgcg gtccagggcg cgaattttaa ctttctctgc gcgtggcact
    18841 aaagcacgtt gttcagggat cgacaggcaa ccttcttcaa tgcctgtttc gccgcttttt
    18901 tctaaaagct ccggattgat taacaccagc cgttcgtcac ggttttccga aacatcaata
    18961 acaatgatac gttgatggat atcaacctgg gttgccgcca ggccaatacc ttcttctgcg
    19021 tacatcgtct cgaacatatc atcgacgata cgctgaattt ctgcattcac ttcttctacc
    19081 ggtttagcaa ctttgcgaag ccgctcgtcc ggaatatgta acacttgcaa aactgacata
    19141 aatctccaga gatgtgttca ggagttagaa agattatttc ttctattcta gacaaatccc
    19201 cctctgattg acagcatcac tgaccaatcg caaagattgc taaggctgct tatggcaggg
    19261 agataaggat ggtcgataca gatatttggc tgcgtttaat gagcatcagc agcttgtacg
    19321 gcgatgatat ggtccgtata gctcactggc tggcaaaaca gtcgcatatt gatgcggttg
    19381 tattgcagca aacagggctt acattgcggc aggcacaacg ctttctttca tttccgcgaa
    19441 agagtatcga aagctcactt tgttggttgg agcaacccaa ccatcattta atccctgcgg
    19501 acagcgaatt ttatcctcct caacttctgg tgacgacaga ttaccccggc gcactgtttg
    19561 ttgaaggaga actgcacgcg ctgcattcat ttcagcttgc cgtagtgggg agtcgggcgc
    19621 attcatggta tggcgagcga tggggacggt tattttgcga aactctggcg acgcgtggag
    19681 tgacaattac gagtggactg gcgcgtggaa tcgatggtgt ggcgcataaa gctgccttac
    19741 aggtaaatgg cgtcagcatt gctgtattgg ggaatggact taataccatt catccccgcc
    19801 gccatgcccg actggctgcc agtctgcttg aacatggcgg agctctcgtc tcggaatttc
    19861 ccctcgatgt tccacccctt gcttacaatt tcccacgaag aaatcgcatt atcagtggtc
    19921 taagtaaagg tgtactggtg gtggaagcgg ctttgcgcag tggttcgctg gtgacagcac
    19981 gttgtgcgct tgagcagggg cgtgaagttt ttgccttgcc aggtccaata gggaatccgg
    20041 gaagcgaagg gcctcactgg ttaataaaac aaggtgcgat tcttgtgacg gaaccggaag
    20101 aaattctgga aaacttgcaa tttggattgc actggttgcc agacgcccct gaaaattcat
    20161 tttattcacc agatcagcaa gacgtggcat tgccatttcc tgagctcctg gctaacgtag
    20221 gagatgaggt aacacctgtt gacgtcgtcg ctgaacgtgc cggccaacct gtgccagagg
    20281 tagttactca actactcgaa ctggagttag caggatggat cgcagctgta cccggcggct
    20341 atgtccgatt gaggagggca tgccatgttc gacgtactaa tgtatttgtt tgaaacctat
    20401 attcacacag aagctgagtt gcgtgtggat caagacaaac ttgaacagga tcttaccgac
    20461 gcaggatttg atcgagaaga tatctacaat gccctgcttt ggctggaaaa gcttgcggat
    20521 tatcaggaag gactggcaga accgatgcaa ctggcctctg atccactctc catgcgtatt
    20581 tatacccctg aggagtgtga aagactggat gccagctgtc gtggttttct gcttttcctt
    20641 gagcaaattc aggtgcttaa ccttgaaacc cgtgaaatgg tgatagagcg agtgctcgcg
    20701 ctggataccg cagagttcga cctggaagac ctgaaatggg taatcctgat ggtgttgttc
    20761 aatattccgg gctgcgaaaa tgcgtaccag caaatggaag aattactctt tgaagtgaat
    20821 gaaggtatgc tgcattaatc atttctttaa ttcagcataa gttgttatgg cgaaatcagc
    20881 actgttcacg gtgcgtaata atgagtcctg cccaaagtgc ggggctgaac tggttattcg
    20941 atccgggaaa cacggtccgt ttcttggatg ctcacagtat ccggcgtgtg actacgtccg
    21001 tcctctgaaa tcttcagcgg atggacatat tgtcaaagtt ctggaggggc aggtttgcct
    21061 tgcatgtggc gcaaatctgg tattacgcca gggacgcttt ggtatgttta ttggttgcag
    21121 taactaccct gaatgcgaac ataccgaact tatcgataaa ccggacgaaa cagcaattac
    21181 atgcccccaa tgccggacgg gccatctggt ccagcgccgc tcccgttatg gcaaaacatt
    21241 tcactcctgt gatcgctacc cggagtgtca atttgccatt aacttcaaac ccatagctgg
    21301 agaatgccct gagtgtcatt atccactact catcgaaaag aaaaccgcgc agggtgtaaa
    21361 acacttttgt gccagtaaac aatgtggaaa gccggtttcg gcggaataat aacgtgaata
    21421 ataacctgca aggagacgct atcgcagctg cgatagatgt cctcaatgaa gaacgtgtca
    21481 tcgcctatcc aacggaagcc gttttcggtg ttgggtgcga tcctgatagc gaaacagcag
    21541 tgatgagact gttggagtta aaacagcgtc cggttgataa ggggctgatt ttaatcgcgg
    21601 caaattacga gcagcttaaa ccctatattg atgacaccat gctgactgat gcgcagcgtg
    21661 aaaccatttt ttcccgctgg ccaggtcctg tcacctttgt ctttcccgcg cctgcgacaa
    21721 caccgcgctg gttgacgggc cgctttgatt cgcttgctgt acgagtcacc gaccatccgt
    21781 tggtggttgc tttgtgccag gcttatggta aaccgctggt ttctaccagt gccaacttga
    21841 gtggattgcc accttgtcga acagtagacg aagttcgcgc acaatttggc gcggcgttcc
    21901 cggttgtgcc tggtgaaacg ggggggcgtt taaatccttc agaaatccgc gatgccctga
    21961 cgggtgaact gtttcgacag gggtaacata atggaaacct atgctgtttt tggtaatccg
    22021 atagcccaca gcaaatcgcc attcattcat cagcaatttg ctcaacaact gaatattgaa
    22081 catccctatg ggcgcgtgtt ggcacccatc aatgatttca tcaacacact gaacgctttc
    22141 tttagtgctg gtggtaaagg tgcgaatgtg acggtacctt ttaaagaaga ggcttttgcc
    22201 agagcggatg agcttactga acgggcagcg ttggctggtg ctgttaatac cctcaagcgg
    22261 ttagaagatg gacgcttgca gggtgacaat accgatggta taggtttgtt aagcgatctg
    22321 gaacgtctgt cttttatccg ccctggttta cgtattctgc ttatcggcgc tggtggagca
    22381 tctcgcggcg tactactgcc acttctttcc ctggactgtg cggtgacaat cactaatcgg
    22441 acggtatccc gcgcggaaga gttggctaaa ttgtttgcgc acactggtag tattcaggcg
    22501 ttgagtatgg acgaactgga aggacatgag tttgatctca ttattaatgc aacatccagt
    22561 ggcatcagtg gtgatattcc ggcgatcccg tcatcgctca ttcatccagg catttattgc
    22621 tatgacatgt tctatcagaa aggaaaaact ccttttctgg catggtgtga gcagcgaggc
    22681 tcaaagtgta atgctgatgg tttaggaatg ctggtggcac aggcggctca tgcctttctt
    22741 ctctggcacg gtgttctgcc tgacgtagaa ccagttataa agcaattgca ggaggaattg
    22801 tccgcgtgaa tcaggccatc cagtttccgg acagagaaga gtgggacgag aataaaaaat
    22861 gtgtatgttt tcccgctctc gtgaatggca tgcaactgac atgtgcgatc tctggcgaga
    22921 gtctggcgta tcgttttact ggagatacgc cagaacagtg gttagcgagt tttcgtcagc
    22981 atcgctggga tctggaagaa gaagcggaaa acttaattca ggaacaaagt gaagatgatc
    23041 aaggctgggt ctggttaccc tgatccagat attcgtcctt ccatttcacg taattattcg
    23101 cggaatagcg taacccagcc ttctcttcat cacttaacgg gcggatctgt ttgacggggc
    23161 taccgagata cagatatccg ctctccagcc gtttattttg tgggaccaga ctacccgcac
    23221 caatcatcac atcatcttct actattgcgc catcaagtaa aattgagccc atcccaacca
    23281 aaactcgatt gccaatggtg cagccgtgga gcatcacctt gtgaccaaca gtgacatctt
    23341 cgccaatggt taatgggttg ccatctgggt tgtacgagga tttatgagtg acatgcaaca
    23401 tactgccatc ctggatattg gtgcgtgctc cgatctgtac ataatgtaca tctccacgaa
    23461 tcacaacgag cggccagatc cccacatcat cagccagacg aacgtcacca atcacgacac
    23521 tgctatcgtc gatcattacg cgctgaccga tttgtggaaa aagatcgcgg tatgggcgta
    23581 aaacatcaga catacttacc tcagcaataa atgatttact aatgactttg ggggcattat
    23641 tggccttgtg caagtctttt agtatgcaaa aaagcaccgt tttgtgtgcg attgcagcaa
    23701 aaagggtgaa aaaacaacaa acagaaaaaa agatcaaaaa aatacttgtg caaaaaattg
    23761 ggatccctat aatgcgcctc cgttgagacg acaacgtgaa acacttcaca agatggtcgt
    23821 aacaacgaag agaaaaaatc ctgaaattca gggttgactc tgaaagagga aagcgtaata
    23881 tacgccacct cgcgacagtg agctgaaag
//
LOCUS       DAMZET010000057        23568 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.057, whole genome shotgun sequence.
ACCESSION   DAMZET010000057 DAMZET010000000
VERSION     DAMZET010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.057"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..870
                     /gene="hemH"
                     /locus_tag="POB34_002324"
     CDS             <1..870
                     /gene="hemH"
                     /locus_tag="POB34_002324"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDI5919261.1"
                     /translation="LSDRRVVDTSRLLWWPLLRGVILPLRSPRVAKLYASVWMEDGSP
                     LMVYSRQQQQALAQRLPDTPVALGMSYGSPSLESAVDELLAEHVDHIVVLPLYPQFSC
                     STVGAVWDELARILARKRSIPGISFIRDYADNHDYINALANSVRASFAKHGEPDLLLL
                     SYHGIPQRYADEGDDYPQRCRTTTRELASALGMAPEKVMMTFQSRFGREPWLMPYTDE
                     TLKMLGEKGVGHIQVMCPGFAADCLETLEEIAEQNREVFLGAGGKKYEYIPALNATPE
                     HIEMMANLVAAYR"
     gene            complement(867..1826)
                     /gene="aes"
                     /locus_tag="POB34_002325"
     CDS             complement(867..1826)
                     /gene="aes"
                     /locus_tag="POB34_002325"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HDI5919262.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQSDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            1978..3282
                     /gene="gsk"
                     /locus_tag="POB34_002326"
     CDS             1978..3282
                     /gene="gsk"
                     /locus_tag="POB34_002326"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HDI5919263.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(3415..5091)
                     /locus_tag="POB34_002327"
     CDS             complement(3415..5091)
                     /locus_tag="POB34_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HDI5919264.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(5329..6549)
                     /gene="fsr"
                     /locus_tag="POB34_002328"
     CDS             complement(5329..6549)
                     /gene="fsr"
                     /locus_tag="POB34_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HDI5919265.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKSKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            6767..8418
                     /gene="ushA"
                     /locus_tag="POB34_002329"
                     /pseudo
     CDS             6767..8418
                     /gene="ushA"
                     /locus_tag="POB34_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
     gene            complement(8455..8934)
                     /gene="ybaK"
                     /locus_tag="POB34_002330"
     CDS             complement(8455..8934)
                     /gene="ybaK"
                     /locus_tag="POB34_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HDI5919266.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(9138..9932)
                     /locus_tag="POB34_002331"
     CDS             complement(9138..9932)
                     /locus_tag="POB34_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HDI5919267.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            10070..10411
                     /locus_tag="POB34_002332"
     CDS             10070..10411
                     /locus_tag="POB34_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDI5919268.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDFWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(10525..13029)
                     /gene="copA"
                     /locus_tag="POB34_002333"
     CDS             complement(10525..13029)
                     /gene="copA"
                     /locus_tag="POB34_002333"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HDI5919269.1"
                     /translation="MSQIIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTAEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            13291..14223
                     /gene="glsA"
                     /locus_tag="POB34_002334"
     CDS             13291..14223
                     /gene="glsA"
                     /locus_tag="POB34_002334"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDI5919270.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGKVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLLNTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            14226..15518
                     /locus_tag="POB34_002335"
     CDS             14226..15518
                     /locus_tag="POB34_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI5919271.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            15643..16050
                     /gene="cueR"
                     /locus_tag="POB34_002336"
     CDS             15643..16050
                     /gene="cueR"
                     /locus_tag="POB34_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HDI5919272.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(16051..16509)
                     /locus_tag="POB34_002337"
     CDS             complement(16051..16509)
                     /locus_tag="POB34_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDI5919273.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(16506..17423)
                     /locus_tag="POB34_002338"
     CDS             complement(16506..17423)
                     /locus_tag="POB34_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDI5919274.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            17569..18246
                     /gene="fetA"
                     /locus_tag="POB34_002339"
     CDS             17569..18246
                     /gene="fetA"
                     /locus_tag="POB34_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HDI5919275.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKDIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            18233..19012
                     /gene="fetB"
                     /locus_tag="POB34_002340"
     CDS             18233..19012
                     /gene="fetB"
                     /locus_tag="POB34_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HDI5919276.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFVAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(19075..19929)
                     /gene="cnoX"
                     /locus_tag="POB34_002341"
     CDS             complement(19075..19929)
                     /gene="cnoX"
                     /locus_tag="POB34_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HDI5919277.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(19990..20799)
                     /gene="ybbO"
                     /locus_tag="POB34_002342"
     CDS             complement(19990..20799)
                     /gene="ybbO"
                     /locus_tag="POB34_002342"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDI5919278.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(20789..21412)
                     /gene="tesA"
                     /locus_tag="POB34_002343"
     CDS             complement(20789..21412)
                     /gene="tesA"
                     /locus_tag="POB34_002343"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HDI5919279.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            21383..22069
                     /locus_tag="POB34_002344"
     CDS             21383..22069
                     /locus_tag="POB34_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5919280.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            22066..>23568
                     /locus_tag="POB34_002345"
     CDS             22066..>23568
                     /locus_tag="POB34_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5919281.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQ"
BASE COUNT         5597 a         6250 c         6272 g         5449 t
ORIGIN      
        1 ttaagcgaca gacgcgtggt tgatacctca cggttgttat ggtggccgtt gctgcgcggc
       61 gtgattttgc cgctgcgctc gccgcgtgtg gcgaagctgt acgcctcggt ctggatggaa
      121 gacggctcgc cgctgatggt ttacagccgc cagcaacagc aggcgttggc acaacgttta
      181 ccggatacgc ccgtagcgct gggcatgagc tacggctcgc catcactgga aagcgccgtc
      241 gatgaactcc tggcagagca tgtagatcat attgtggtgc tgccgcttta tccgcaattc
      301 tcctgttcta cggtcggtgc ggtatgggat gaactggcac gcattctggc gcgcaaacgt
      361 agcattccgg ggatatcgtt tattcgtgat tacgccgata accacgatta cattaatgca
      421 ctggcgaaca gcgtacgcgc ttcttttgcc aaacatggcg aaccggatct gctgctgctc
      481 tcttatcatg gcattcccca gcgttatgca gatgaaggcg atgattaccc gcaacgttgc
      541 cgcacaacga ctcgcgaact ggcttccgca ctggggatgg caccggaaaa agtgatgatg
      601 acctttcagt cgcgctttgg ccgggaaccc tggctgatgc cttataccga cgaaacgctg
      661 aaaatgctcg gagaaaaagg cgtaggtcat atacaggtga tgtgcccggg ctttgctgcg
      721 gattgtctgg agacgctgga agagattgcc gagcaaaacc gtgaggtctt cctcggtgcc
      781 ggcgggaaaa aatatgaata tattccagcg cttaatgcca cgccggaaca tatcgaaatg
      841 atggctaatc ttgttgccgc gtatcgctaa agctgagcgg taaagaactg agcgccgtcg
      901 cgaagagcct cgtcggcggt tttcatcatt cgtgaataat gcaaaaaggc atgcagcgta
      961 cctgggtaga gtttgaactc acagggctgc tgatgcgccg ctaacgtctg gtaaagcaga
     1021 cggctgtcat ccagcagcgg atcgaactcc gccccggcaa taaaacaggg cggaacttcg
     1081 cgagtgagat cattattaaa cagacagtaa tacggtgact cgcggtccgc gtcgttgctt
     1141 aaatacgcct cttcatacat ctgcaaatcc tgttgcgtta agccatccca gacaccgccc
     1201 aacagacgac gagtcacgga atcccgtaat ccgtaaagcc cataccacag caaaacaccc
     1261 gcaattttac cgcaatcgat ctgtttatca cgcaaccaca acgcactggc gagcgccagc
     1321 atggcacctg cggaatcacc ggcaaagcca atgcgggaca tattgatttg ataatcctcc
     1381 gcctgctggt ggaaataaca acaagcagcc acaatttcct ctatcgcttg cggaaaacgc
     1441 gcttcaggtg aaagggggta atcaatacca atcaccgtac attggctgta gcttgccagc
     1501 aggcgcatga tgcgatcgtg ggtatcgaga ttgccgagaa taaaaccgcc tccatgcaaa
     1561 taaaatagcg tcgccgggct atctggctgc ggacaaaaga gacgtgtttc cacctgccca
     1621 tattttgttg gaaccatgta agctctggtt gccatttctg gagcgcccgc attccagaat
     1681 cggcgctcaa gcgtgtaata ctgtcgttgc tcagcaatcg ttcccgttgc gggccaggac
     1741 ggtaaatccg actgaagagt attcacaacg gtcttcattt cagcagaaat aaggtccaga
     1801 acaggtagtt tgttttccgg cttcatacaa aaactccttt caaattacgt cattgtaagg
     1861 aaccactgcc atgaaaatgc gatcccgcct gctgatattg aaactggctg cgtctcgcgc
     1921 gctcccgtca gattgtgtta acattcgccg ctcagttaac cacccgtaaa aacaaccatg
     1981 aaatttcccg gtaaacgtaa atccaaacat tacttccccg taaatgcacg cgatccgctg
     2041 cttcagcaat tccagccaga aaacgaaacc agcgctgcct gggtagtggg tatcgatcaa
     2101 acgctggtcg atattgaagc gaaagtggat gatgaattca ttgagcgtta tggattaagc
     2161 gccgggcatt cactggtgat tgaggatgat gtagccgaag cgctttatca ggaactaaaa
     2221 cagaaaaacc tgattaccca tcagtttgcg ggtggcacca ttggtaacac catgcataac
     2281 tactcggtgc tcgcggacga ccgttcggtg ctgctgggcg tcatgtgcag caatattgaa
     2341 attggcagct atgcctatcg ttacctgtgt aacacctcca gccgtaccga tcttaactat
     2401 ctacaaggcg tggatggccc gattggtcgt tgctttacgc tgattggcga gtccggggaa
     2461 cgtacctttg ctatcagccc tggccacatg aaccagctgc gggctgaaag tattccggaa
     2521 gatgtgattg ccggagcctc ggctctggtt ctcacctctt atctggtgcg ttgcaagccg
     2581 ggtgaaccca tgccggaagc aaccatgaaa gccattgagt acgcgaagaa atataacgta
     2641 ccggtggtgc tgacgctggg aactaagttt gtcattgccg agaatccgca gtggtggcag
     2701 caattcctca aagaccacgt ctctatcctt gcgatgaacg aagatgaagc cgaagcgttg
     2761 accggagaaa gcgatccgtt gttggcatct gacaaggcgc tggactgggt agatctggtg
     2821 ctgtgcaccg ccgggccaat cggcttgtat atggcgggct ttaccgaaga cgaagcgaaa
     2881 cgtaaaaccc agcatccgct gctgccgggc gctatagcgg aattcaacca gtatgagttt
     2941 agccgcgcca tgcgccacaa ggattgccag aatccgctgc gtgtatattc gcacattgcg
     3001 ccgtacatgg gcgggccgga aaaaatcatg aacactaatg gagcggggga tggcgcattg
     3061 gcagcgttgc tgcatgacat taccgccaac agctaccatc gtagcaacgt accaaactcc
     3121 agcaaacata aattcacctg gttaacttat tcatcgttag cgcaggtgtg taaatatgct
     3181 aaccgtgtga gctatcaggt actgaaccag cattcacctc gtttaacgcg cggcttgccg
     3241 gagcgtgaag acagcctgga agagtcttac tgggatcgtt aagttatcgt cggttcgtag
     3301 gccagataag gcgttcacgc cgcatctggc atttggctct cgatgcctga tgcgacgctg
     3361 gcgcgtctta tcatgcctac atatttttca tattttacat ccggcaacca ccgtttaccc
     3421 cgtcaccacc tcacccgccg gtggcgtttc cagcagttcc agcatggtac gggcgatttc
     3481 acgctcgccc atcactacct gattcgcacc acgttcggtg atatacgcca cttcatcgtc
     3541 ataatgggcg cgggcaataa tctcaatatc cggatttttc gcgcgggcag atgccacaat
     3601 ctcacccgct tcataaccgt tgggaatcgt caggatcagc cattttgcac attccagatg
     3661 cgccagttgc ataatttctt cgttcgccgc attgcccaat actgcgcgga ccccgcgctc
     3721 tcgcagctca tcaacacggg ttcgtgacgt ctcaatcacc accagcggaa tatcagaggc
     3781 gagcaatttc tcccccagca ggctgcctac acgaccgtaa cccaccagta gcgcatggtt
     3841 gcaaatatcc actgggatct gcttctcttc ttcgattgcc tcttccagcg tctgctcttc
     3901 cagcgtttcg gtcttcgcca gatatttctc cagtagtgcg aacagtaccg ggttgagcat
     3961 aatcgacagg atcgcccctg ccagtaccag gttttgtccg gcctgcggca gtaaattcaa
     4021 tgccattccc agtcccgcca ggataaacgc gaactcacca atctgcgcca ggctggcggc
     4081 gatggttaat gccgtacgtt gggagtgacc aaacagtcgc accaggaaaa atgcggctaa
     4141 cgacttacca aacagaataa tcgccagcgt cgccagcact gccagcggtt gctgaatcag
     4201 aattaacgga tcaaacaaca tcccgacgga gacaaaaaac agcaccgcaa acgcgtcgcg
     4261 caatggcagc gtatcgtggg cggcacggtg actcagttca gactcgttca gtaccatccc
     4321 ggcaaagaac gcaccgagtg caaaggagac atcaaacagc tctaccgcac caaaggcaat
     4381 ccctaacgcc agcgccagca ccgacagggt aaacagctcg cgagaaccgg ttgccgcgct
     4441 gcgtgccata atccacggca ccagacggcg acctaccagc atcataatgg cgataaatgc
     4501 gatcactttg ccgatggtga tccccatatc gactgcaaga gtggcaaagc ccacatcgcc
     4561 ctgttccatc attcctgcca ctgcgggcag caacaccagc gtcagaacca ttaccaggtc
     4621 ttccacaatc aaccaaccga tggcgatttg cccacgctga ctgtcaatta attgccgttc
     4681 ttcaagtgcg cgcagtaaca ccacggtact ggcggtggaa agacataaac cgaacacgat
     4741 accggtcatt aacgaccagc ccagcacggc agagagcgcc atacccagca gcgtcgccac
     4801 ggctatctgg gcgatcgcac cgggaatggc gatggccttt accgccatca aatccttcag
     4861 cgaaaagtgc aaaccgacgc caaacatcaa cagaatgacg cccagttcag ccagttccgg
     4921 cgcaagcttg gtatcggcaa caaagcccgg agtgaatggt cctgccagca cacccgctaa
     4981 cagatatccc accagaggag aaatacgtag tttattggcc agcatgccga ggataaaggc
     5041 gagcacaagg ccgccaacaa tggtggtgat aagcggggtg gcgtgatgca ttccgtctcc
     5101 ttttcctggt ggttattgtc catttttggc cgggaaaacc aaaattacag gtaatagttt
     5161 atgacaattt cattgatgat gttcatgaat aattattgaa ttttgcagaa aaatggaatt
     5221 agctgcaaaa aaagcacgga tcggaaaaca gaaggcggtt taacagagga aaggcttatg
     5281 gcgactgtaa aacgatgcag ccaaagtttg cctttggctg caatgaaatc agtctttatg
     5341 ccggttatca ggcaggaata tggtcaacat ccccaatagt ggcaggaaag cacagatttt
     5401 atagactaac tcgatgctgg tgtgatcggc gataagcccc agaactgccg ctcccagacc
     5461 tcccatgcca aaagcaaaac cgaaaaagag tccagaaacc ataccgatac gtcccggaag
     5521 tagctcctga gcgtagacca gaatggcaga gaatgccgaa gcgaggataa atccaataat
     5581 caccgttaaa acccccgtcc agtgcaggct ggcgtagggt aaaatcagcg taaacggcgc
     5641 aacgccgagg atagagcccc aaatcacata tttccgtcca attttatccc ctacaggccc
     5701 gccgatcacc gtacctgccg caacggcaaa caggaaggca aacagatgaa gctgggcatt
     5761 ctggatagat aatccgaatt tttgcatcag ataaaaggtg taatagctgc tgatgctcgc
     5821 catatagaaa tatttcgaga aaatgaggat taacagaatg ctgactgcca gtaccacttt
     5881 gttgcgcggc agtggattga ttatcgtcgc tttggatttt cctttattca ttcggtgctg
     5941 tgccgagtac caacggctaa tttgcgccaa caccacgatc gccagcagtg ccgcaagcac
     6001 aaaccaggca acgttgcctt tgccataagg cgcgataatc accgccgcca gcaagggtcc
     6061 cagggaactg ccaaagttgc cgccgacctg aaagatagat tgcgccaggc catgccgccc
     6121 gccggaagcc atacgggcta cgcgagaaga ttccggatga aagaccgatg aaccggtacc
     6181 gaccagcgcc gccgccagca gaactgcgcc aaaactgccc gccagcgcaa gcagcaccag
     6241 accacttaag gtaaagcaca tgccaattgg caacgaccac ggcatcggat atttatcggt
     6301 ccagtagccg accactggtt gcagtagcga agaggcgagc tggaaggtga gggttatcat
     6361 gccaatctgc ataaatgtca gagaaaattc tgactgaagc agcggataaa tcgccagaat
     6421 cagcgattgg atcatgtcgt tcagcagatg tgagaggctg atagcaccta aaataccaaa
     6481 cgatgttcgg gccttggtcg ttgacgcagc cgcgcccgcc acaggctggg gttgttcact
     6541 cattgccata ggaaagtcac tttttcaggg ttgcgatgta aagaatgatc ttatttgtga
     6601 ttattacccg actaacatac ctgtatgcgt cgcctgaagg aagtctcaac gccgaataca
     6661 gaatttctaa tctggatgca gatttatctt caccggacgc agacttgtct atgatgtcgc
     6721 gtcatactat ttttcaacac gttgaaatca ggtcagggag agaagtatga aattattgca
     6781 gcggggcgtg gcgttagcgc tgttaaccac atttacactg gcgagtgaaa ctgctctggc
     6841 gtatgagcag gataaaacct acaaaattac agttctgcat accaatgatc atcatgggca
     6901 tttttggcgc aatgaatatg gcgaatatgg tctggcggcg caaaaaacgc tggtggatgg
     6961 tatccgcaaa gaggttgcgg ctgaaggcgg tagcgtgctg ctactttccg gtggcgacat
     7021 taacactggc gtgcccgagt ctgacttaca ggatgccgaa cctgattttc gcggtatgaa
     7081 tctggtgggc tatgacgcga tggcgatcgg taatcatgaa tttgataatc cgctcaccgt
     7141 attacgccag caggaaaagt gggccaagtt cccgttgctt tccgcgaata tctaccagaa
     7201 aagtactggc gagcgcctgt ttaaaccgtg ggcgctgttt aagcgtcagg atctgaaaat
     7261 tgccgtcatt gggctgacaa ccgatgacac agcaaaaatt ggtaacccgg aatacttcac
     7321 tgatgtcgaa tttcgtaagc ccgccgatga agcgaagctg gtgattcagg agctgcaaca
     7381 gacagaaaag ccagacatta ttatcgcggc gacccatatg gggcattacg ataatggtga
     7441 gcacggctct aacgcaccgg gcgatgtgga gatggcacgc gcgctgcctg ccggatcgct
     7501 ggcgatgatc gtcggtggtc actcgcaaga tccggtctgc atggcggcag aaaacaaaaa
     7561 caggtcgatt acgtgccggg tacgccatgc aagccagatc aacaaaacgg catctggatt
     7621 gtgcaggcgc atgagtgggg caaatacgtg ggacgggctg attttgagtt tcgtaatggc
     7681 gaaatgaaaa tggttaacta ccagctgatt ccggtgaacc tgaagaagaa agtgacctgg
     7741 gaagacggga aaagcgagcg cgtgctttac actcctgaaa tcgctgaaaa ccagcaaatg
     7801 atctcgctgt tatcaccgtt ccagaacaaa ggcaaagcgc agctggaagt gaaaataggc
     7861 gaaaccaatg gtcgtctgga aggcgatcgt gacaaagtgc gttttgtaca gactaatatg
     7921 gggcggttga ttctggcagc ccaaatggat cgcactggtg ccgactttgc ggtgatgagc
     7981 ggaggcggaa ttcgtgattc tatcgaagca ggcgatatca gctataaaaa cgtgctgaaa
     8041 gtgcagccat tcggcaatgt ggtggtgtat gccgacatga ccggtaaaga ggtgattgat
     8101 tacctgaccg ccgtcgcgca gatgaagcca gattcaggtg cctacccgca atttgccaac
     8161 gttagctttg tggcgaaaga cggcaaactg aacgacctta aaatcaaagg cgaaccggtc
     8221 gatccggcga aaacttaccg tatggcgaca ttaaacttca atgccaccgg cggtgatgga
     8281 tatccgcgcc ttgataacaa accgggctat gtgaataccg gctttattga tgccgaagtg
     8341 ctgaaagcgt atatccagaa aagctcgccg ctggatgtga gtgtttatga accgaaaggt
     8401 gaggtgagct ggcagtaatc cgaaagtgcc ggatgtttgc atccggcaca atgcttaatc
     8461 gcggcgggcg atatcagcaa atttggcatc gaggatcttt gccagatcgc ctgccgccag
     8521 ttcgatatcc agtccgcgct tgccgccgga aacataaata gtggcaaatt cttgtgcggg
     8581 ggcgtcgata atcgttggca gacgtttttt ctgccccagt gggctaatcc ccccaaccag
     8641 gtatcccgtc gaacgctgcg cgaccatcgg atcggccatc tcaactttct tggcacccag
     8701 cgcttttgct acttttttaa gatccagttg acctgcaacc ggcgtaacgg ccactgccag
     8761 atgtttcata tcaccgttca ctgccaccag cagcgttttg tagacctgat ccggattcaa
     8821 acctaatttt ttgacgactt catcgccaaa attggtttca gccggatcgt gctcgtaggt
     8881 atggatttga aacgaaatct tgtttttttc gagtaattta actgcgggtg tcatggtaat
     8941 tttcctgcct taaacaaaaa aacgaagcaa gcatacgcct gatggcggtc caaaaaaaag
     9001 agtcatcttg cctaagagta ttggcaggat ggtgagattg agcgacaatc gagttacacc
     9061 gtcgcttaaa gtgacggcat aataataaaa aaatgaaatt cctctttgac gggccaatag
     9121 cgatattggc cattttttta gcgcaacatt tgcggcaaat tcccttctcc atacaggtgt
     9181 agtgcaccga ccgcgaccac atatcgcccc ggcggcatgg cgcgtaattt atcccgccag
     9241 gcgagatttc gctgatgcat cagcacatcg tacagcgact gactgaacgt attgggcagc
     9301 gttatttcat tattttgcgg cggcgcattc agccaccagc tcatcatttg ttgcagcaac
     9361 cgtgcgttgg tatgccagtg ggtcagcgta tcgtccagca gcgccagtcc tttgtcaggg
     9421 agctggagca acatggtaat ctggttttca gccccttcca gttcaatcac gggtttatgt
     9481 tgttgcttcg ccgcctgcaa taactggtaa tcgataccgt attctgcccg cagccccagt
     9541 ttttgtgcct gcgtcgcctg aagaaccatc gcgatttgcc acagcggttg agtagaaaag
     9601 agcgacggag aaatgcccat ctcctggcta atgtgctgca gattttggag ttgctcctcg
     9661 ttaatgcgct cttccagcgc ctcgcaagta ggcaaattag caaaaggcgt atcgctggtg
     9721 gaaacatccg cctcgacgat cagcgcatcg gcgtttttga gctttttgag caaacgggtg
     9781 ggcaggggag ccatatcgtg gctacccata tgaatactgc caatcagatg aaaatggcga
     9841 ttgccgggaa gggtgatatc gatggcaggc caggtgtaat gattaccgcg cagcgcggcc
     9901 caaagcgttt ttacccggta caacagatcc atacgacctc cctttgtgaa atatcatgct
     9961 agcgcgcggt gagggatggc gcaaatggag ttggggactt gtttaatgtg tttgtatgat
    10021 tcagtatgtt cttgcatcgc tattcacagg gaagcaacag ttaaaaacca tgaaacaggc
    10081 aacaagaaaa ccgacgacac ccggagatat tcttctctat gaatatctgg aaccgctcga
    10141 tttgaaaatc aatgagttag cagagttgct gcatgttcat cgtaatagcg tcagtgcact
    10201 gatcaataac aatcgtaaac tcaccactga gatggcattc cgtctggcga aagtttttga
    10261 taccacagtc gattttaggc taaacctcca ggcggcagtt gatttttggg aagttgaaaa
    10321 caacatgcgc acccaggaag aattgggacg gattgaaaca gtggctgaat atttggcacg
    10381 ccgtgaagag cgtgcaaaaa aggtcgcgta aggccaaaac atcgcttcat ctcgtaggcc
    10441 ggataaggcg tttacgccgc atccggcagt catgcgtcga tgccaaatgc gccgccctaa
    10501 agcagcgcat ccgcaatgac gtacttattc cttcggttta aaccgcagca accggttggc
    10561 gttactcact acggtaatcg acgagagcgc cattgccgct ccggcaacta ccgggttaag
    10621 cagtgttcca gtaaacggcc acaaaatacc ggcggcgacc ggaataccga tgctgttgta
    10681 gataaaggca ccgagcaggt tctgcttcat gttgcgcagc gttgcgcggg aaatcgcgag
    10741 cgcatccgca acgcccatca ggctatggcg catcagggta atcgccgcgg tttcaatggc
    10801 aacatcactg ccgccaccca tcgcaatgcc gacatccgcc tgggccagcg ctggcgcgtc
    10861 gttaatgccg tcgcccacca ttgccacctg acgtccttca ctttgcagac gtttgatcgc
    10921 ttcggcttta ccgtccggca gcactccggc aatcacttca tcaattccag cttctttggc
    10981 gatcgcattg gcggtggttg ggttatcccc ggtcaacatc accagacgat atcccgcttt
    11041 atgcaggcgt tgcagcgccg ccacgctatc actacgcaac ggatcgcgta cagccagcag
    11101 ggctactgct ttcccgtcaa tcgccagcag cacaggcgtt gctccttgcg atgcctgagc
    11161 agtaatctcc gcttcgatag ctttggtacc aacctgttgc tcatttaaca gcgcctgatt
    11221 gcccagcaat aacgcatgac cttcagcttc accgctcacg cccagcccgc gcaatgtgcg
    11281 gaaaccgttg acctgcggca gctgcatatc acctgcttta tcgaggatcg cccgtgccag
    11341 cggatggctg gaaccttgct ccagtgccgc cgccagacgc aatgcctgcg cttcatcaac
    11401 atcagcaaac gttttcactg cgacaacctg cggcttccct tcggtcagcg tcccggtttt
    11461 atcgaacacg acggtatcga gtgtactggc gcgttgcagc gcgtcggcgt cccgcaccag
    11521 cacgccaaac tcagccgccc gcccgacgcc ggaaataatc gacatcggcg tcgccagccc
    11581 cagcgcacac ggacaggcga taatcagtac cgtggtggca atcaccaggg tataaacaat
    11641 ttgcggtgcc ggaccaaaga agtaccagat tgccgcactg accagcgcaa tcaccaccac
    11701 cacaggtaca aatacggctg agattttatc cgccagctga ccgatttctg gcttgctgct
    11761 ctgggcctgg cgcaccatgc gaatgatccg tgacagcgtg gtatggctgc caaccgcact
    11821 ggcacgaaac agcacactgc cgtcctgtac cactgtcccg gcatggacgc tttcgccttc
    11881 gcctttttgc tgtgggattg gttcgcccgt cagcatcgct tcatccagcc atgcttcgcc
    11941 ctgggtaatc tcgccatcta ccggcacgcg atcgccggtc gtcaggcgca gtaacatccc
    12001 tggctgaact tccgccagcg gcacgctttt ttcaccctct gcggtgacca ctcgcgccgt
    12061 cggtggggtt aaatcgagta atttctccag cgccttagaa gaacgctgac gtgcgcgcgc
    12121 ttccagcata tggccgagat taatcagacc gataatcatc gcactggctt catagtaaag
    12181 atgccgcgct tccatcggga accactgcgg ccacaggttg acgctcatcg aatagagcca
    12241 cgccacgcca gtacccagcg ccaccagcgt atccatcgtc gctgcaccgt tcagcaggct
    12301 tttccatgca ctgcggtaaa aatggccgcc ggcgaaaacc atcaccgcca gggttatcag
    12361 gccgataacc aaccacaggc tgcggttgtc agcggtgacc atcatgttat cgccaatcat
    12421 cccccagacc atcaccggga tacccaccgc cagtgcgaca attgcctgcc agcggaagcg
    12481 cttcatcgta gcaacggcgg tttcctgctg gcgctcacgg cgtttcgcgt catcttcaat
    12541 cgcttccgcg ccgtagcccg ctttttccac cgcctgcact aaatcttgtg gggaggcact
    12601 gcccatcacc agcgcagtac gctccgccag gtttacccgt gcctgagtga cgcccggtac
    12661 gctttgcagc gcattttgta cgcgggtgac acagctggcg cagctcatgc cgctcagcag
    12721 caactgctgg ctgtcatcgt catcggcggt cgctgccgga agcgcctcag aaaccgctgt
    12781 cagtgcttcc gacgggattg atgactccgc cagcggttta gcctttgggt ggcttacaga
    12841 tgcgtcataa cccgcttgtt tgatggtttc gatcagctgt tctgcactgg cagtcccggt
    12901 aacgtgcgct tcagtgatag acacatccgc ttgttccaca tccgggcgct gttcgagact
    12961 ttctttcacg cgtttaacgc agtgaccgca ggacaggccg tccagggtca ggtcgataat
    13021 ttgtgacata aaacactcct ttaagacagt tttgactggc tgtgataaag gttaaacctt
    13081 ccagcaaggg gaaggtcaag aaattaataa accaggcgag taaaagtccg taaagattaa
    13141 aaaattggct caatttgcat caggattaga catttatctc tttgttttcc tgtagttaag
    13201 ttgcgggtgc taagttaaat caggatgcct gaaaatcggc accggggtga ggaattacct
    13261 cccgcatcta taaaaaggag ttaacaaaag atgttagatg caaacaaatt acagcaggca
    13321 gtggatcagg cttacaccca atttcactca cttaacggcg gtcaaaatgc cgactacatt
    13381 ccctttctgg cgaatgtgcc tggtcaactg gcggcagtgg ctatcgtgac ctgcgatggc
    13441 aaggtctata gtgcgggtga cagtgattat cgctttgcgc tggaatccat ctcgaaagtc
    13501 tgtacgttag cccttgcgtt agaggatgtc ggcccgcagg cggtacagga caaaattggc
    13561 gctgacccga ccggattgcc ctttaactca gttatcgcct tagagttgca tggcggcaaa
    13621 ccgctttcgc cactggtaaa tgctggcgcc attgccacca ccagcctgat taacgctgaa
    13681 aatgttgaac aacgctggca gcgaatttta catatccaac agcaactggc tggtgagcag
    13741 gtagcgctct ctgacgaagt caaccagtcg gaacaaacaa ccaacttcca taaccgggcc
    13801 attgcctggc tgctgtactc cgccggatat ctctattgtg atgcaatgga agcctgtgac
    13861 gtgtacaccc gtcagtgctc cacgctcctc aatactgttg aactggcaac acttggcgcg
    13921 acgctggcgg cggggggagt gaatccgctg acgcataaac gcgttcttca ggccgataac
    13981 gtgccgtaca ttctggccga aatgatgatg gaagggctgt atggtcgctc cggtgactgg
    14041 gcatatcgcg ttggtttacc gggcaaaagc ggtgtaggtg gcggtattct ggcggtcgtc
    14101 cctggtgtga tgggaattgc cgcgttctca ccaccgctgg acgaagaagg caacagtgtt
    14161 cgcggtcaaa aaatggtggc atcggtcgct aagcaactcg gctataacgt gtttaagggc
    14221 tgatcatgat gaacacggaa ggtaataacg gtaacaaacc cctcggtcta tggaacgtcg
    14281 tttccatcgg cattggggca atggtggggg cggggatctt cgcgctgctg gggcaggctg
    14341 cgttgctgat ggaagcctcg acctgggtcg cctttgcttt tggcggtatt gtggcgatgt
    14401 tttccggtta tgcctatgcg cgtctggggg cgagctatcc cagcaatggc ggcattatcg
    14461 acttctttcg tcgcggatta ggcaacggcg tcttttcgct ggcgctctcg ttactgtacc
    14521 tgttgacgct ggcggtgagc atcgccatgg tcgcccgtgc ttttggcgct tatgccgtgc
    14581 agtttttgca tgaaggcagc caggaggagc accttatttt gctctacgcg ttggggatca
    14641 ttgcggtgat gacgcttttc aactccttaa gcaaccatgc ggtagggcgg ctggaagtga
    14701 tcctcgtcgg cattaaaatg atgatcctgt tattgctgat tattgccggt gtctggtcgc
    14761 tgcaaccggc gcatatttcc gtctctgcgc cccccagctc cggtgcgttc ttctcctgta
    14821 tcgggataac cttccttgcc tatgcgggct ttggcatgat ggcgaacgcg gcggataaag
    14881 tgaaagatcc gcaggtcatt atgccgcggg cgtttctggt ggcgattggc gttaccacgt
    14941 tgctttatat ctcgctggca ctggttttgc ttagcgatgt atcggcatta gagttagaaa
    15001 aatatgccga taccgccgta gcgcaggctg cttctccgct gctcggacat gtgggttatg
    15061 tgatcgtcgt catcggcgct ttactggcga cggcttcagc cattaacgcg aacctgttcg
    15121 ccgtgtttaa catcatggac aacatgggca gcgaacgcga actgccgaag ctaatgaata
    15181 aatccctgtg gcggcagagt acctggggca acatcattgt cgtggtgttg attatgctga
    15241 tgacggcggc gctaaattta ggctcactcg ccagtgttgc cagcgccacc tttttgattt
    15301 gctacctggc ggtgtttgtg gtggcgatcc gcctgcgtca tgatattcac gcctcgttgc
    15361 cgattcttat cgttggtacg ttggtgatgt tgttggtgat cgttggcttt atctacagtc
    15421 tgtggtccca gggtagccgt gcgttgatat ggattattgg ctcactctta ctcagcctta
    15481 ttgtggcaat ggtcatgaag cgcaataaaa ccgtataaca tctctctgtg cgcagtactt
    15541 cctgtattat tgtggtggcg gtcgatattc gcactggcaa aaaaacgtgc ttgaatatct
    15601 gttgaaaccc tttaacaaag cacaggaggc gttgcgcgaa cgatgaacat cagcgatgta
    15661 gcaaaaatta ccggcctgac tagcaaagcc attcgcttct atgaagagaa ggggctggtg
    15721 acgccgccga tgcgcagcga aaacggttat cgcacctaca cgcagcagca tctcaacgaa
    15781 ctgaccttac tgcgccaggc acggcaggtg ggctttaacc tggaagagag cggcgagctg
    15841 gtgaatctgt ttaacgaccc gcagcggcac agcgccgacg tcaaacggcg cacgctggag
    15901 aaggtggcgg agatcgaacg acacattgag gagctgcaat ccatgcgcga ccagctgctg
    15961 gcactggcga atgcctgccc tggcgatgac agcgccgact gcccgattat cgaaaatctc
    16021 tccggctgct gtcatcatcg ggcagggtga ttaagacgag acggctcgga tatgtagggt
    16081 tatcccttct atcgcaatga cttcaacatg cgtacctgca ccgagatcct cgctggcgct
    16141 gacaggccat gaactgtcac cgacgcgcat atgaccgcgc ccgttgacca gcggagattc
    16201 cagcacaaaa cgtcggccaa tcagctgctg cccgcgctgg tttaaatgac tatcgctgtg
    16261 cttttgttcg cgcacccgcc gcgacaacca tttccaccac agccaggcgg cgagcagcgt
    16321 caggatggca aacattaccc cttgccactc ccaacccagc ggcaccagcc agaccaccag
    16381 gccggtaatc accgctgcca cgccgctcca caacaaataa ccatttccgc ccagcatctc
    16441 ggctgccagc agcaaaccgc cgagactgag ccagaaaata tgtggatgaa cgaccattaa
    16501 ctccatcatg gctgagtccg tttgttggcg ctgtctttca ccagctcggc aatcccggca
    16561 atcgacccca tcaggctgct ggcctctaat ggcatcatca ccactttgct gttactggac
    16621 gaaccgatct gctgtaacgc ttcggtgtat ttctgcgcta cgaagtagtt caccgcctga
    16681 atatcacctg aggcgatggc ttcagacacc attttggtgg cgcgggcttc tgcttcagcg
    16741 gaacgttcac gcgcttcagc ctgtaaaaac gccgactgac gttcgccttc cgctttcagg
    16801 atttgcgact gtttttcacc ttcggctttg aggatttccg cctgacggat cccttccgct
    16861 tcaaggatgt aagcgcgttt ggtacgttcc gctttcatct gcgcgttcat tgaagagata
    16921 agctctgccg gtgggcgcac gtcgcgaatt tcaatacggg tgactttaat cccccacggg
    16981 ttggtggctt catcgacaat acgcagcagg cgtgagttga tgctgtcgcg ctgagagagc
    17041 atttcatcaa gttccattga acccagcacg gtacggatgt tagtcatggt caggttgatg
    17101 atagccagct ccagattgct gacctcataa gccgcgcgtg gcgcgtcaat cacctgaata
    17161 aagcacacgg cgtcgatggt aacgttggcg ttatctttcg agataacttc ctgggaaggg
    17221 atatcgagca cttgctccat catattgatc ttgcgaccaa tgcgatccat aaacggcacc
    17281 accagactga gccccggctg taacgttttg gtatagcgac caaaacgttc taccgtccat
    17341 tgatagccct gcggcacgat tttgacgccc gcgccgacaa tgaccagcgc gacaaaaatg
    17401 agaatcggga taaagataag catcggaaaa acctcctgtt gtaccgtcca taatcagcaa
    17461 aattgctgct tgattaaaca aattatacct gattactgaa agagagttcc cccttattcc
    17521 tgcgaaggat aaactgtttt tagtaaaaat cagaaaaagg gaacagcgat gcaggaaaat
    17581 agtcctttgc ttcagctaca aaacgtagga tatctggcgg gtgatacgaa gattcttaat
    17641 aacatcaatt tttcgctgcg tgctggcgaa tttaagttaa ttaccggtcc ttctggttgt
    17701 ggcaaaagta cgctgctaaa aatagttgct tcattgatca gcccaaccag cggaacgtta
    17761 ctgtttgaag gggaggatgt cagcacacta aagccagaaa tctaccgcca gcaagtctct
    17821 tactgcgccc agacaccgac gctgtttggc gatacggtat acgataatct gatctttccc
    17881 tggcagatcc gtaaccagca gcctgacccg gccatttttc tcgattttct cgaacgcttc
    17941 gctttgccgg acagcatttt gacgaaggat atcgccgagc tatctggtgg tgaaaaacaa
    18001 cgcatctcat tgattcgtaa cctgcaattt atgccgaagg ttttattgct ggatgaaata
    18061 accagtgcgc tggatgaaag taataaacat aacgtcaatg agatgatcca tcgttatgtg
    18121 cgcgagcaaa atattgccgt gctgtgggtg acacacgata aagacgaaat taatcatgcg
    18181 gataaagtga ttacactgca accgcatgcc ggagaaatgc aggaagcacg ctatgaactc
    18241 gcataatatt actaacgaat cattagcact ggcattaatg ctggtggtgg tggcaatctt
    18301 aattagccat aaagaaaaac tggcgctgga gaaagatatt ctctggagcg tcgggcgagc
    18361 gataattcag ctgattattg tcggctatgt gctgaagtat attttcagcg tggatgatgc
    18421 cagcctgaca ttattgatgg tgttatttat ctgctttaat gcggcgtgga acgcgcaaaa
    18481 acgcagtaaa tatattgcta aagcttttat ctcatcgttt gttgccatta cggtcggggc
    18541 gggaattacc ctggcggtgc tgattctctc agggtcgatt gaatttatcc cgatgcaggt
    18601 gatccctatc gccgggatga ttgccggtaa cgccatggta gcggtggggt tgtgttacaa
    18661 caatttaggg caacgggtca ttagcgaaca gcaacagatt caggagaaac tgagtcttgg
    18721 tgcgacgccg aagcaggctt cagcgatatt gattcgcgac agtattcgcg cggctttaat
    18781 tccgacggtc gattcagcaa aaacggttgg cttagtgagt ttaccaggaa tgatgtccgg
    18841 gctgatattt gccgggattg atccggtgaa ggcgattaaa tatcagatta tggtgacctt
    18901 tatgctgctc tcaaccgcca gcttgtcgac cattattgcc tgctatttaa cctatcgtaa
    18961 gttttataat tcgcgccacc agttggtggt gacgcaattg aagaagaaat aatgcgatgc
    19021 cggatgcgca gcatcgcatc cggcgttgtg gttcatgtgc cgatcaacgg aatatcaata
    19081 caacaatgca tacagctggc ggcgatactt cgacgccagt gcatcacccg tacccagcgc
    19141 agcgaggatc tcctggaacg ttttacgcgt ctgaccgtct gcggcggtga gatctttacg
    19201 cagatgcccg aacagcaact ccagcgcctc ttcattgcgc ccaacctggt gcagttgcag
    19261 agccagttgc gtcgccagtg cggcatcttc tggattctcc gccacctgct gttgcaactg
    19321 ttgaatttcc ggcgtatcag ccgcctgctt cagcagttcg atttgcgcca ccagcccctg
    19381 gtagcgggtg tcctgatcct gcaacggaat ggttttcagc accgcttccg catcttcaga
    19441 acggttcagc gcaatcagcg tttctgccag cagcaggccg atctccccgt tctgattcga
    19501 caactgccag gcgtctttca gcaatggcag ggcatcggtg taattgcctt cctgcatcag
    19561 ttgcatcgcc tgctgcgctt tcagctcttc ttcgcgcggc agcactttat ccagcagggc
    19621 gcggatcgcc tcttccggtt gcggcccctg gaagccatct accggttgcc cgttctggaa
    19681 cagatacacg gtcggaatcg cgcgcagacc aaactgggcg gcaatcatct gctccgcgtc
    19741 gcagtccagc ttcgccagaa taaattgccc gttgtactgc gccgcgaggc tttccaggat
    19801 cggggttaac tgcaaacagt gctggctacg ttcagaccaa aaatagaaca gcaccggagt
    19861 ggtcatcgac tgttcaagaa cctgttgcag gttagattcg ttaatgttga caatattttc
    19921 tacggacatg gagtcgctct ctgttgtcga tattttcttt gacatggggg cttaagcgcg
    19981 cgcttcaact cacccctgca atattttgtc catcacgcgc cccggcagca ggcgcttaag
    20041 caccattact gcccaggtca ccagcgttac cggatagcgc atcttcggct tctcgctaat
    20101 aaaagcatgg cgtactttgt ccaccaccgc ttccggtccc aacgtaaagc gggcggcgat
    20161 gccgggattt tcgactggtt tatcactttg cgtctggttg acgttgtcgg tgaagcgagt
    20221 acgaatggga ccgggttcga tcaggctgac tttaattccg ctgtggcgta gctccatgcg
    20281 cagcgcgtct gaccacgcct ccagcgcata tttactggcc gcgtaagcgc cacgacccgg
    20341 cgtggagatt aatcccatca ccgatgatgt catcacaata cgcccttcac cgtgcggtaa
    20401 catcgcgggt aacaggcgca tggtgagctg gtgtgcgccg aaaaagttgg cggaaaactg
    20461 ctgttccatc tgcgcacggc tgatggtgga aagggggcca tacatgccga atccggcatt
    20521 gttaaagatc ccatacagac aattatcggt cagggcgatc acttcgtcgg ctgcgcgatc
    20581 aacactttct ggcgaatcca gatcgatcaa cacgccggta aatcccatgc tgttcatacg
    20641 ctcaacatca tccggtttcc ggcaacctgc cagcacatga aaaccctggc gttttaattc
    20701 gagcgcgctt tccaggccaa ttccactgga acatccggta attaagaccg atttttgcat
    20761 aactttacct gtcaggatct ccgttgcttt atgagtcatg atttactaaa ggctgcaact
    20821 gcttcgccat ccagtcggca ataaacggct gggcgtcgcg gttgggatga ataccgtcat
    20881 cctgcatcca ttgtggcttg aggtagacct cttccataaa aaagggcagc agcggaacat
    20941 caaactcttt ggcgagtttg gggtaaatgg cgctaaaggc ttcattataa cggcgaccat
    21001 agtttgcagg cagacgtatt tgcattaaca atggttcagc gttggcggct ttgacatcct
    21061 gcaaaatctg gcgcagcgtt tgctcggttt gctgtggctg aaaaccacgc aaaccgtcat
    21121 tgccgcccag ttcaaccagc acccaacgcg gctgatgctg tttcagcaga gccggaaggc
    21181 gcgccagtcc ttgttgcgag gtgtcgccgc tgatgctggc attaactacc gacgttttac
    21241 tctgccactt atcattcaac aaggcaggcc aggccgcgct ggcagacatt cgatacccgg
    21301 cgctcaggct atcacccaga atcaataacg tgtccgctgc ggcggcacgg aaggttaaca
    21361 ggaccaggaa caggaagggc aaatgccagc ggaaaacatt gttgaagttc atcatcttaa
    21421 gaagtccgtc ggtcaggggg agcatgaact ctccatcctc accggagttg agctggttgt
    21481 caaacgtggc gagaccatcg cactggtggg cgagtcggga tcgggtaagt caaccttgct
    21541 ggcgatcctc gccgggcttg atgacggcag cagtggcgaa gtgagtctgg tgggacaacc
    21601 gctacataat atggacgaag aagcgcgggc aaagttgcgc gcgaagcacg tcggctttgt
    21661 ttttcagtca tttatgttaa ttcctaccct taacgcgctg gaaaacgtcg agcttccggc
    21721 tctgctgcgc ggtgagagta gcgcggaaag tcgtaacggg gcgaaagcgt tgctcgaaca
    21781 gttagggctg ggtaaacgtc tggatcatct tccggcacag ctttccggcg gtgaacagca
    21841 acgagtggcg ctggcacgag cctttaatgg tcgacctgat gtgctgtttg ccgacgaacc
    21901 caccggcaac cttgaccgcc agacgggcga taaaattgcc gacctgctgt tttccctcaa
    21961 ccgtgaacat ggcaccacgt tgattatggt gacccacgac ctgcaactgg cggcacgctg
    22021 cgaccgctgc ttacggctgg tgaacgggca gttgcaggag gaagcatgat tgcacgttgg
    22081 ttctggcgcg aatggcgttc gccgtcgcta ttaattgtct ggctggcgct aagcctggcg
    22141 gtggcctgcg tgctggcgct gggcaatatc agcgatcgca tggagaaggg cttaagccag
    22201 caaagccgtg agtttatggc gggcgatcgg gcgttgcgca gttcacgcga agtgccgcaa
    22261 gcgtggctgg aggaagcgca aaagcgcggc ctgaaagtcg gcaagcagct gactttcgcc
    22321 acaatgacct ttgcaggcga cacaccgcag ctggcgaacg tcaaagcggt ggatgatatc
    22381 tacccgatgt atggcgatct gcaaactaat ccccctggcc tgaaaccgca ggcgggcagc
    22441 gtattgctgg ccccacgcct gatggcactg cttaacctga aaacgggcga caccattgac
    22501 gtgggcgatg ccaccttgcg gattgccgga gaagtgattc aggaaccgga ttccggtttt
    22561 aaccccttcc agatggctcc gcgtctgatg atgaatctgg cggatgtcga taaaaccgga
    22621 gccgtgcagc cggggagtcg ggtcacctgg cgttataaat tcggcggcaa cgagaaccag
    22681 ctcgacggct atgagaaatg gttgttacct cagcttaaac ccgaacaacg ctggtacggt
    22741 ctggaacagg acgaaggcgc gctggggcga tcgatggaac gctcgcaaca gttcctgctg
    22801 ctttcggcgc ttctgacctt gctgctggca gtggcagcgg tggcggtagc gatgaatcat
    22861 tactgtcgca gtcgctacga tctggtggcg atcctcaaaa cgctgggggc agggcgagcg
    22921 caactgcgta agctaatcgt cggtcagtgg ttgatggtgc tgacgctttc agccgttacc
    22981 ggtggggcca taggtctgtt gttcgaaaac gtgttgatgg tgctgctcaa gcccgttctg
    23041 cctgctgcac taccaccagc cagcctctgg ccgtggctgt gggcgcttgg caccatgacg
    23101 gtcatctcgc tgctggtggg gctacgacca taccgcttgt tgctggcaac gcagccttta
    23161 cgcgtattac gtaatgatgt ggtagcgaac gtctggccgc tgaagtttta tctgccgatt
    23221 gtcagtgtgg tggttgtgct gctgctcgcc ggattaatgg gtggcagcat gctgctttgg
    23281 gcggtgctgg cgggcgcggt agtactggct ttgctgtgcg gtgtgctggg ctggatgctg
    23341 ctgaatgtac ttcgccgcat gacgctgaaa tcgctgcctc tgcgcctggc ggttagccgc
    23401 ctgttacgtc agccgtggtc aacgttaagt cagctttcgg cattttcgct ctcctttatg
    23461 ctgctggcgc tgttgctggt attgcgtggc gatctgctcg accgctggca gcagcagcta
    23521 ccgccagaaa gcccgaacta ctttttaatt aacatcgcca cagaacag
//
LOCUS       DAMZET010000058        23258 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.058, whole genome shotgun sequence.
ACCESSION   DAMZET010000058 DAMZET010000000
VERSION     DAMZET010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.058"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1664
                     /locus_tag="POB34_002346"
     CDS             <1..1664
                     /locus_tag="POB34_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HDI5919282.1"
                     /translation="TTAVRLNVMGEKTHDAGRDKVKNERYGVAPSVAFGLGTANRLYL
                     NYLHVTQHNTPDGGIPTIGLPGYSAPSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDT
                     ATMRFEHDINDNTTIRNTTRWSRVKQDYLMTAIMGGASNITQPTSDVNSWTWSRTANT
                     KDVSNKILTNQTNLTSTFYTGSIGHDVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDS
                     SIHPGGLTRNGANANGQTDTFAIYAFDTLQITRDFELNGGIRLDNYHTEYDSATACGG
                     SGRGAITCPTGVAKGSPVTTVDTAKSGNLMNWKAGALYHLTENGNVYINYAVSQQPPG
                     GNNFALAQSGSGNSANRTDFKPQKANTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQ
                     NDDGTYSQYGKKRVEGYEISVAGNITPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPY
                     TPEHAFTLWSQYQATDDISVGAGARYIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRV
                     NRNLDFQLNVYNLFDTDYVASINKSGYRYHPGEPRTFLLTANMHF"
     gene            1706..2383
                     /gene="ybiX"
                     /locus_tag="POB34_002347"
     CDS             1706..2383
                     /gene="ybiX"
                     /locus_tag="POB34_002347"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HDI5919283.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            2457..2723
                     /gene="ybiI"
                     /locus_tag="POB34_002348"
     CDS             2457..2723
                     /gene="ybiI"
                     /locus_tag="POB34_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HDI5919284.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            2988..3248
                     /locus_tag="POB34_002349"
     CDS             2988..3248
                     /locus_tag="POB34_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDI5919285.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(3477..4562)
                     /gene="hcxB"
                     /locus_tag="POB34_002350"
     CDS             complement(3477..4562)
                     /gene="hcxB"
                     /locus_tag="POB34_002350"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HDI5919286.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(4703..5665)
                     /gene="ybiB"
                     /locus_tag="POB34_002351"
     CDS             complement(4703..5665)
                     /gene="ybiB"
                     /locus_tag="POB34_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HDI5919287.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            complement(5693..7843)
                     /gene="dinG"
                     /locus_tag="POB34_002352"
     CDS             complement(5693..7843)
                     /gene="dinG"
                     /locus_tag="POB34_002352"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HDI5919288.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            7963..8445
                     /gene="ybiA"
                     /locus_tag="POB34_002353"
     CDS             7963..8445
                     /gene="ybiA"
                     /locus_tag="POB34_002353"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HDI5919289.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            complement(8677..10041)
                     /gene="rhlE"
                     /locus_tag="POB34_002354"
     CDS             complement(8677..10041)
                     /gene="rhlE"
                     /locus_tag="POB34_002354"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HDI5919290.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            10270..10941
                     /gene="cecR"
                     /locus_tag="POB34_002355"
     CDS             10270..10941
                     /gene="cecR"
                     /locus_tag="POB34_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HDI5919291.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            10944..11939
                     /gene="hlyD"
                     /locus_tag="POB34_002356"
     CDS             10944..11939
                     /gene="hlyD"
                     /locus_tag="POB34_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HDI5919292.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLILA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            11932..13668
                     /locus_tag="POB34_002357"
     CDS             11932..13668
                     /locus_tag="POB34_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5919293.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVDGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            13661..14794
                     /locus_tag="POB34_002358"
     CDS             13661..14794
                     /locus_tag="POB34_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5919294.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            14805..15911
                     /locus_tag="POB34_002359"
     CDS             14805..15911
                     /locus_tag="POB34_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5919295.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(15873..16283)
                     /locus_tag="POB34_002360"
     CDS             complement(15873..16283)
                     /locus_tag="POB34_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HDI5919296.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRPPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            16416..17177
                     /locus_tag="POB34_002361"
     CDS             16416..17177
                     /locus_tag="POB34_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDI5919297.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            17174..18415
                     /gene="clsB"
                     /locus_tag="POB34_002362"
     CDS             17174..18415
                     /gene="clsB"
                     /locus_tag="POB34_002362"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HDI5919298.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            18415..19371
                     /locus_tag="POB34_002363"
     CDS             18415..19371
                     /locus_tag="POB34_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HDI5919299.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(19407..20120)
                     /locus_tag="POB34_002364"
     CDS             complement(19407..20120)
                     /locus_tag="POB34_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDI5919300.1"
                     /translation="MESYSQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPTMYVAIGLLLVCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAP
                     VPAIIVVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERP
                     VWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(20325..21029)
                     /locus_tag="POB34_002365"
     CDS             complement(20325..21029)
                     /locus_tag="POB34_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDI5919301.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(21166..21618)
                     /gene="moaE"
                     /locus_tag="POB34_002366"
     CDS             complement(21166..21618)
                     /gene="moaE"
                     /locus_tag="POB34_002366"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HDI5919302.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(21620..21865)
                     /gene="moaD"
                     /locus_tag="POB34_002367"
     CDS             complement(21620..21865)
                     /gene="moaD"
                     /locus_tag="POB34_002367"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HDI5919303.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(21858..22343)
                     /gene="moaC"
                     /locus_tag="POB34_002368"
     CDS             complement(21858..22343)
                     /gene="moaC"
                     /locus_tag="POB34_002368"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDI5919304.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(22346..22858)
                     /gene="moaB"
                     /locus_tag="POB34_002369"
     CDS             complement(22346..22858)
                     /gene="moaB"
                     /locus_tag="POB34_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HDI5919305.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREIEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(22880..>23258)
                     /gene="moaA"
                     /locus_tag="POB34_002370"
     CDS             complement(22880..>23258)
                     /gene="moaA"
                     /locus_tag="POB34_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="together with moaC, is involved in the conversion
                     of a guanosine derivative (GXP) into molybdopterin
                     precursor Z; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDI5919306.1"
                     /translation="KHHISGQVLRDELLRRGWIHQLRQRSDGPAQVFCHPDYAGEIGL
                     IMPYEKDFCATCNRLRVSSIGKLHLCLFGEGGVNLRDLLEDDTQQQALEARISAALRE
                     KKQTHFLHQNNTGITQNLSYIGG"
BASE COUNT         5518 a         6289 c         6050 g         5401 t
ORIGIN      
        1 ataccaccgc ggtgcgcctg aatgtaatgg gcgaaaaaac gcacgatgcc ggacgcgaca
       61 aagtcaaaaa tgagcgttac ggcgtcgccc cttctgtcgc ttttggcctt ggtacagcga
      121 atcgtttgta tcttaattat ctgcatgtca cccagcacaa cacgccagac ggcggcattc
      181 cgaccatcgg tttgccgggc tattctgccc catctgcggg aacggcggcc ctgaatcatt
      241 ccggaaaagt tgatactcat aacttttacg gcacggattc cgattacgac gattcgacca
      301 ccgacaccgc caccatgcgt tttgagcacg acatcaacga taacaccacc attcgcaata
      361 ctacccgttg gtcgcgcgta aagcaggatt acctgatgac ggcgattatg ggcggggcgt
      421 cgaatattac tcagcccacc agcgatgtga atagctggac ctggtcacgc acggcgaata
      481 ccaaagatgt gagtaataaa attctcacca accagaccaa cctgacctcg acgttctata
      541 ccggttctat cggtcatgat gtcagtaccg gcgtggaatt tacccgtgaa acgcagacta
      601 actacggcgt taatccggtg acgttacccg cggtaaatat ttatcatcct gacagcagca
      661 ttcatcccgg cggcctgacg cgcaacggcg caaacgccaa tggtcagacg gataccttcg
      721 caatttacgc ctttgatacg ctgcaaatca cccgtgattt tgagctgaac ggcgggatcc
      781 gtctggataa ttatcatact gaatatgaca gtgccaccgc ctgcggcggc agcggacgcg
      841 gtgccatcac ctgcccaact ggtgtggcaa aaggttctcc ggtcaccacc gtcgacaccg
      901 ccaagtcggg caatctgatg aactggaaag ccggggcgct gtatcacctg acggaaaacg
      961 gcaatgtcta tattaactat gccgtttccc agcagcctcc gggcggcaac aacttcgccc
     1021 ttgcgcagtc tggcagcggt aacagtgcca accgcaccga ttttaaaccg caaaaagcca
     1081 acaccagcga gattggcacc aaatggcagg ttctggataa acgtctgttg ctcaccgccg
     1141 cgctgttccg tactgatatc gaaaatgaag ttgagcaaaa tgatgacgga acttactcgc
     1201 aatacggtaa gaaacgcgtc gaaggctatg agatatccgt ggccgggaat atcactcccg
     1261 cgtggcaggt gattggcggc tatacccagc aaaaagcaac catcaaaaac ggcaaagatg
     1321 ttgcccagga tggttcctca tcgctgccgt ataccccgga gcacgccttc accttatgga
     1381 gccaatatca ggcaaccgac gatatctctg ttggcgcggg cgcacgctat atcggcagta
     1441 tgcataaagg ttcagacggc gcggtgggaa cgccagcgtt taccgaaggt tactgggtcg
     1501 ccgatgccaa actggggtat cgagttaatc gcaatctcga cttccagcta aacgtttaca
     1561 acctgtttga taccgattac gtcgcctcaa tcaacaagag cggctaccgt tatcacccgg
     1621 gcgagccaag aaccttcttg ctcacagcca atatgcattt ctgattcaga tgtggggcgc
     1681 aggccccact ttttggagaa attgtatgat gtaccacatt cccggcgtgt tatcgccaca
     1741 ggacgtcgct cgttttcgcg aacaactgga acaagccgaa tgggtggatg gacgcgtcac
     1801 caccggcgca caaggtgcgc aagttaagaa caatcaacag gtcgacaccc gcagcacgtt
     1861 atacgccgcc ctgcaaaatg aggtgctgaa cgcggttaac caacatgctt tattctttgc
     1921 cgcggccttg ccgcgtaccc tttccacgcc gctgtttaat cgctatcaga acaatgaaac
     1981 ctatggtttc catgtggatg gcgcagtacg cagccatcca caaaacggct ggatgcgtac
     2041 tgacctttct gccacgctgt ttttaagcga tccacaaagc tacgacggcg gcgaactggt
     2101 cgttaatgac accttcggac aacatcgggt aaaactcccg gcaggcgatc tcgtgttgta
     2161 tccctccagc agcctgcatt gcgtgacacc cgtaacccgc ggcgtacgag tggcatcatt
     2221 tatgtggatc cagtcgatga tccgcgatga taaaaagcgc gccatgctgt ttgaactgga
     2281 caacaatatt cagtcgctga aaagccgcta cggcgaaagt gaagagatcc tgtcgctgct
     2341 taatctttat cataatctgc tgcgggaatg gtcggagatc tgatgcttga actgcccgag
     2401 ttcgtccctg gattagctac acttaactgt acaagtattg atatggggag gtcgatatgg
     2461 catccggttg ggctaacgat gacgccgtca acgaacagat caacagtaca attgaagatg
     2521 cgattgcccg cgctcggggt gaaattccgc gcggcgaaag cctggatgaa tgtgaagagt
     2581 gcggtgcccc catcccgcag gcccgtcggg aagccattcc tggcgtgcgc ttatgtattc
     2641 attgtcagca ggagaaagat ttacaaaaac cagcttatac aggatataat cgcagaggtt
     2701 cgaaagacag ccagttacgt taactacaac tggcgagatg catagcgagt ataatttgta
     2761 ttttgcgtgg cattccgtgc ctttaacgct gctgtgactg ccacgcggta taaaacaagt
     2821 tcataagtac aacaaataaa tggtttatca gtaggttaga tatcaatcaa tttatttgaa
     2881 caaggcggtc aattctcttc gattttatct ctcgtaaaaa aacgtgatac tcatcacatc
     2941 gacgaaacaa cgtcacttat acaaaaatca cctgcgagag attaattatg aaaactatca
     3001 atactgttgt tgctgctatg gctctttcaa ctctgtcatt tggcgtgttc gcggcggaac
     3061 cggtaacggc atcccaggca cagaacatga ataaaatcgg cgtggtttct gccgatggcg
     3121 catccaccct cgatgccctg gaagcgaaac tggctgagaa agccgcggca gccggtgcca
     3181 gtggatacag catcacttcc gccaccaaca acaataaatt aagcggtact gcggtaattt
     3241 ataagtaatt cgtaggacgg ataaggcgtt tacgccgcat ccggcaacag atgcctgatg
     3301 cgacgctacc gcgtcttatc aggcctacaa aatccgaacc gttggacgga tcaggcgttt
     3361 acgctacgtc cagcacaagt atttccctgt ctgtttgccg acagacgcat atgctctaac
     3421 cctcattgat cctatgttac ccttgtttgc ccgtccgcca ctggacgggc ttttttttag
     3481 ctggctaact gctgacagaa agcctgcaac gtctcttccg gcataccaat ctgccgcgct
     3541 gcatcacaaa tggcctgcca gcttcccgca tccagtggaa tcccctgctt ctgccgttcg
     3601 cgacgcgtgt tcacttccca ctcgcccggt agcaaaatcg gcttatcatc atcatgcggc
     3661 gaggctttca cccactcggc aaaggcttcg gtctgcgcgt tacaatccgg cgcgccgaag
     3721 agttccgggt tgatgatgat agtggtcatg cagttaagaa tggcatcggg actggtttgt
     3781 aacgtttcct gatgcgtcgt tttaccgccg gaaagcgccc cgccaagaat ttcacacatc
     3841 gctgcaaggg cgtagccttt atgttcggca aaggtcaaca gcgaacccaa cggcgactcc
     3901 tgcattaccg ccggattggt cgtcggcacg ccgttaacgt caatcaggca acctggcggc
     3961 acggggacgc ctttatgcca ggcgacgcgg gttttgccaa atgcaatggc gctggtggcg
     4021 taatcaagca acagcgggaa attatcttta cgagggaaaa ccacacagaa cggattggtg
     4081 ccaaagcggc tgtcgcgacc gtggaacggc gcgaccattg gaataccgac cacgctaaca
     4141 aagtggatag agacaaaccc cgccgctgca cactgctccg cccagtaacc gatacggccg
     4201 atatgatgcg agttatgtag cgccacggcg gcaataccgt gctgatgcgc tttctcaatc
     4261 cccagcgcca tcgcttcatg tgccgcgacc tgaccaaatg cgcgatcgcc atcgagcgtg
     4321 accgccgccc ccgcctcttt aacggttttg gcatgatggt taatttgcag gtgcccctga
     4381 ctccaggagc gtacatagct tgggatcatg ccaataccat gtgaatcatg ccctgccagg
     4441 tttgccgcga ttaaatgatc ggcaactaat ttcgcttctt gttcctcgct acccatctga
     4501 cgaaatacag cctgaataaa actgtgcagc gtctgagcat caaagcgatg accactttcc
     4561 atggttaact cctgtttatg ttatgtgttt gttgtatttt tatgctgcac gccgggcgtg
     4621 ggaaggtcaa taaagatgca ggaatttcat attgcggtgg ggtaaaagcc ggatgacatg
     4681 gctcatccgg ctgagaaaag aattaaaatg cctgattaac gcgcgccagg ccgtcgctga
     4741 tagttgccgc ttcacccgta gccaccaggc agcaagccat ctggattttc agcgattcgg
     4801 gaatcggttc gctgccagca aggcaacgct caatccactg cgccgtggtt tccggatctt
     4861 ttgcttgtgg cagtaactcg ctaccagcag tgtcctgttt ttcatacagc acccgcatcc
     4921 cttcacggtc aatgagattg atctgcgggc agcgctgcgg attagcatac acttcacctt
     4981 cagtaccatg cattagtagc gcccgcccgc cgatatcgct aaagaacttc gcgacgcgtc
     5041 caatgtattc cggatgcgaa acgctggaaa gacgcagcgc ctcaccttcg gcaaatggcg
     5101 tcgccagttt cgccagggta tgtgcactgt tacgcacgcc catccgccag cgcatcgcca
     5161 gttgtttttc cagcggcggg caaaacgcgc cgactggcat aaacaccggt tgatgttcgt
     5221 cgagcttcgc ctgcgcctgc ccgccgtgca acgttggcgt aatacccatc aattcaaaaa
     5281 tggtttcagt cagcacgcgg gttggatctt cgctaacccc gtgaaccacc acaggaaaac
     5341 cgagtttgtg caggagaatc gccaacaacg gcgtcaggtt ggcctgttta cgcgcgccgt
     5401 tgtaactggg gatgacaatc ggcatcggct tgcctgctgg cggcgtcagc ttgatggtgt
     5461 gattttgcat ggcttcgtaa aagccgagca tctctgcttc cccttctcct ttgatacgca
     5521 gcgcaatcaa tacgccgccc aactcaaggt cagggacttc accattgagc atatgagcat
     5581 acagaccgcg cgcagtatcc cggtctaaat cgcgcgcgtg gtttttcccg cgcccgatct
     5641 ctttaatgat tttgcgatag tccatttacg actccttacc tgactcacat cattaacgcc
     5701 gccgacggcg tggggatttc gttttttctt tctttttaac tataccttca gggacttccg
     5761 gttgctctat cggaaatacc ggtaatgcat ccagtagtcg cttgccataa tttttggtca
     5821 gcaagcgttt gtcgtagata accacttcgc cccagcaacc gtggcttcga atcagtcgcc
     5881 caacctgctg aatcaggtta aacgaggcgc tcggcaggct ttgcacctca aacggatagc
     5941 ggttgaggct tttcagccat tccccttcgg tgatcaccac cgggctgtcg atgggcggaa
     6001 aagcgatttt gtggatatgc acctggctga gcagatcacc tttcaaatca agcccttcgg
     6061 caaatgactg taagcccacc agcacgctgc gctcaccgtt ggcgacgcgt ttgcggtgca
     6121 gttcaactaa acggtaacgc ggctgatcgc cctgaaccag caacatcaga cgtaaatccg
     6181 tcacatagtc gagaaagcgc tgcatcgccc gtccgctggc aaacagtacc aacataccga
     6241 gatgtttttt gctctccacc tgcttacgga aaaaggccgc catttcggca atatgctgct
     6301 cttcgttgtc gatggaaggc tcaacgcgca tccggggaat aacaattttg ccctgttcgc
     6361 agtggttaaa gggggaatcc agcgccacaa aacggtcgcc cgctttctct ttcagaccac
     6421 tcatctcctg caaacgcgaa aaactgttca gcgaacgcaa ggttgcggag gtgacaataa
     6481 tgtgcggaat actgcgccac agcagccttt ccagctgatc gctgacacgt attcccacgc
     6541 agtgaaacca gaggtgtagc tgcccttcgc gctcttcccg cgtcgcccat ttggtcaccg
     6601 gtgcaccgga agattgcgcc agcgaagcca gccgccagag tttgctttgc gcctcgaaca
     6661 tccccaacgc gcggttcatc tgcaaaatca accgatgcag acgtacaatg tcatggctgc
     6721 cggttttctc acttaaatcg ttaagaaata actccgccag gccacgcagc atctcggtga
     6781 gttttgccag ccgctggcag atctccagca cttcatctgg cagttcgccc atcgcaaaac
     6841 ggtgctctgc ctcctgcccg gcaggcatgt agagattgag aatgttgttt aacgaggcga
     6901 taagctcata caactcttca caatgcgcat tcaaacgttc agggatcgcc agcggtggga
     6961 tggtcttcgg gcgaaactgc tccatgcagg tagcgaccag tttcgtgaac aagtccagct
     7021 gtagtcgata ccagggcgcg gtgatttcag cgctcatctc cagcgcatcc cgggccacat
     7081 ctggcaggtg atgaccttcg tccagcacca gcagtaaatt tttcgggtca ggcaataccg
     7141 cttcgctttc catcgccgcc atcaccagcg catggtttgc caccaccact tccgcttcct
     7201 gaatctcccg acgagcgaca aaaaacgggc attcacgata gtagtaacaa ttgcggttga
     7261 ggcagctggc tttgtcggta cttaaacgac gccagagatc gtcatctata gcgatatcag
     7321 tatgatcgcg cagaccatcc catttataag tgtcgagatc gcccttcagc ttcgcacaac
     7381 gtttttgctc ttcctgattg ttcggcgtca gttcgtcgtc aagaaacgcc agcagatcct
     7441 gttgcgtggg ctcagtgctg gcaagcgccg tcagattacg cgggcaaacg tagcgcccac
     7501 gaccaaaagc ggcagtgaat ttaagatcgg gaatgatctt tttcagcagc ggtaaatctt
     7561 tgctgtaaat ctgatcctgc aacgccacgt tggcggtact caccaccagc gttttttgct
     7621 cttcgcgggc aatggcgatg ccggggatca aataggagag cgttttccca acgccggtgg
     7681 gggcttcaat cgccagatgc cgcccttctt ctccggccag cgttttggcg acgtccgcaa
     7741 tcatctgccg ctgcggcgca cggggaataa agtcggggat ctgttcctga agcgccttat
     7801 accaggcggc aatttgcgct ttaagcgcgg cggttaatgc catgagaaaa cctgaaatac
     7861 tgtataaaca gccaatattg tggcattttt cggttttcag gggtaatggt ttttctgttt
     7921 ctggaaagcg gctcgcaata accaccaaca taatgcggtt taatgcccgt tcgagcacaa
     7981 aggatccaac acgtcatgca agacaccatc ataaatttct acagcaccag cgatgactac
     8041 ggtgattttt ctaactttgc cgcctggccc atcaaggttg acgggaaaac ctggcccacc
     8101 tcagaacact attttcaggc acaaaaattc cttgatgaaa aataccgcga agagatccgt
     8161 cgggtttctt cccctatggt cgccgcacgc atggggcgcg atcgttctaa gcctctgcgt
     8221 aaaaactggg agtcggtcaa agaacaggtg atgcgaaaag cacttcgcgc taaattcgaa
     8281 cagcatgcag aactgcgtgc gctcttgctg gcaaccgcgc ccgcaaaact ggttgagcat
     8341 acggaaaacg atgcttactg gggagacggt ggtaatggta agggcaagaa tcgactgggc
     8401 taccttttaa tggagttgcg cgaacaattg gctatagaga agtaacgtct cggtggctta
     8461 tgggcggtaa aacagataaa ttttttcccg tgttctcaaa ttgctgacaa gtgcttgttg
     8521 tgaaatgccg gatgcggcgt aaacgcctta tccggcctac aaaagcatgc aaattcaata
     8581 tattgcagag ttcacgtagg cctgataagc gtagcgcatc agacagtttt gcgtttgttc
     8641 atcagcctga tgccgggcat agcccggcat aaaagattac tgcgcagcgg caggtttacg
     8701 cggacggcgg cgacgttgtt gttcgcctgc cggtttggca tcgccgaggc ggcgagacgg
     8761 tttttctgca ggtttcgcgc ttgcagattt tgcgccacct tccccacggc gtggttgctg
     8821 ttgaccgcga ccaccacctt gcccacgacc gccgccgcca cgttgctggc gaccgttctg
     8881 gatcggttcg gctttgattg acgggtccgg ctcatagccc ggaatcgcaa tgcgcgggat
     8941 ctcttttttc agcagttttt cgatatcacg cagcagtttg tgttcatcaa cacacaccag
     9001 cgacaacgct tcaccggtag cagccgcacg accggtacgc ccgatacggt ggacataatc
     9061 ttccggtacg tttggcagtt catagttgac gacgtgcggc aactcttcga tatccagacc
     9121 gcgcgcagcg atatcggtcg ccaccagtac gcgaatgtca cccgatttaa aatcagccag
     9181 cgcacgggta cgcgcacctt gcgatttatt cccgtggatc gccgcactac ggatgccatc
     9241 tttattgagc tgctcagcca gatggttagc gccgtgtttg gtacgggtaa acaccagcac
     9301 ctgctgccag ttccctttac caatcatgtg cgacagcaat tcgcgtttgc gtttcttatc
     9361 gacaaagtga acgtgctggg tcacctgatc agacgcggta ttgcggcgtg ccacttcgat
     9421 ttccagcggg ttgtgcaaca gtttttccgc cagggcttta atatcgtcag agaaggtcgc
     9481 agagaacagc aggttctggc gtttcgctgg cagttttgtt aacacgcgac ggatatcgtg
     9541 gataaagccc atgtcgagca tgcggtcagc ttcatcgagg acgaggattt caacctgatc
     9601 cagcttcact gcattttgat gttccaggtc cagcaaacgt cccggtgttg ccaccagaac
     9661 atcaacgccg ccgcgcagct tcatcatttg cgggttaatg ctgacaccgc caaacaccac
     9721 cagcgaacga atgttcaaat atttgctgta atcacggacg ttttcgccaa tctgcgcagc
     9781 cagttcacgg gtcggggtaa gaatgagcgc acgtaccgga cgacgccctt tggcgtgcgg
     9841 ctggcgagtg atcaggtgtt gcaacagcgg cagcgtaaag cccgctgttt taccggtacc
     9901 ggtctgggcg ctagccatca ggtcgcggcc ttccagcacc gcagggatcg cctgctgctg
     9961 aataggggtg ggttcacggt aaccctgctc ggcaacggcg cgcaggatat cagggcttaa
    10021 acccaaagaa tcgaaagaca taactactcc gaaccgcccc gaccgttacc ggtgtagttt
    10081 tcagggagat actgaaaagg aaaatgacaa aaaccacaat gtcatgaggg agcggagtgt
    10141 agcagttttt gtgacgcagc gcataaatta tccctgtgac tggcgtagct aaaattctta
    10201 gccatactgg acaactccca tatggagtca taatcttaat caatcatttg attaagaatg
    10261 gactgtgcga tgaataatcc tgccatgaca atcaagggtg aacaggcgaa aaaacagctg
    10321 attgctgccg cactggcgca gtttggtgaa tatggaatga acgccaccac ccgcgagata
    10381 gccgcccagg ccgggcagaa tatcgctgcc atcacctact acttcggttc gaaagaagat
    10441 ttgtacctcg cctgcgccca gtggattgcc gattttattg gcgagcagtt ccgtccgcat
    10501 gccgaggaag ccgaacgctt gttcgcccaa ccacagcctg atcgggctgc catccgtgaa
    10561 ctgatccttc gcgcctgcag gaacatgatt aagctgctca cccaggatga caccgtcaac
    10621 ctcagcaagt ttatctcccg tgagcagctc tctcccacgg cagcctacca cctggtgcac
    10681 gaacaggtga ttagtccgct acacagccac ctgacacgcc tgattgccgc ctggaccggc
    10741 agcgacgcca atgacacccg catgatcctt catacccatg cgttgattgg tgagattctg
    10801 gcgttccgtc ttggcaaaga aacaattctg ttacgtaccg gctggaccgc gttcgatgaa
    10861 gaaaagaccg aactgatcaa ccagacggtg acctgtcata tcgacctgat tttgcaagga
    10921 ttatcgcaaa ggagtttgta gtgatgaaaa aacctgtcgt gatcggactg gcggtagtgg
    10981 tacttgccgc cgtggttgcc ggaggctact ggtggtatca aagccgccag gataacggcc
    11041 tgacgctgta tggcaacgtg gatattcgta cggtgaatct tagtttccgt gttggggggc
    11101 gcgttgaatc gctggcggtg gacgaaggtg atgctatcaa agcgggccag gtgctgggcg
    11161 aactggatca caagccgtat gagattgccc tgatgcaggc gaaagcgggt gtttcggtgg
    11221 cgcaggcgca gtatgacctg atacttgccg ggtatcgcga tgaagaaatc gctcaggccg
    11281 ccgcagccgt aaaacaggcg caggccgcct atgactatgc gcagaacttc tacaaccgcc
    11341 agcaagggtt gtggaaaagc cgcaccattt cggcaaatga cctggaaaat gcccgctcct
    11401 cgcgcgacca ggcgcaggca acgctgaaat cagcacagga taaattgcgt cagtaccgtt
    11461 ccggtaaccg tgaacaggac atcgcccagg cgaaagccag cctcgaacag gcgcaggcgc
    11521 aactggcgca ggcggagttg aatttgcagg actcaacgtt gatagccccg tctgatggca
    11581 cgctgttaac gcgcgcggtg gagccaggca cggtcctcaa tgaaggtggc acggtgttta
    11641 ccgtttcact aacgcgtccg gtgtgggtgc gcgcttatgt tgatgaacgg aatcttgacc
    11701 aggcccagcc ggggcgcaaa gtgctgcttt ataccgatgg tcgcccggac aagccgtatc
    11761 acgggcagat tggtttcgtt tcgccgactg ctgaatttac cccgaaaacc gtcgaaacac
    11821 cggatctgcg taccgacctc gtctatcgcc tgcgtattgt ggtgaccgac gccgatgatg
    11881 cgttacgcca gggaatgccg gtgacggtgc aattcggtaa cgaggcagga catgaatgat
    11941 gccgttatca cgctgaacgg cctggaaaaa cgctttccgg gcatggacaa gcccgccgtc
    12001 gcgccgctcg attgtaccat tcacgccggt tatgtgacgg ggctggtggg gccggatggt
    12061 gcaggtaaaa ccacgctaat gcggatgctg gcgggattac tgaaacccga cagcggcaat
    12121 gccacggtga ttggctttga tccaatcaaa aacgacggcg cgctgcacgc cgtgctcggc
    12181 tatatgccgc agaaatttgg tctgtatgaa gatctcacgg tgatggagaa cctcaatctg
    12241 tacgcggatt tgcgcagcgt caccggcgag gcacgtaagc aaacttttgc tcgcctgctg
    12301 gagtttacgt ctcttgggcc gtttaccgga cgcctggcgg gcaagctctc cggtgggatg
    12361 aaacaaaaac tcggtctggc ctgtaccctg gtgggcgaac cgaaagtgtt gctgctcgat
    12421 gaacccggcg tcggcgttga ccctatctca cggcgcgaac tgtggcagat ggtgcatgag
    12481 ctggcaggcg aagggatgtt aatcctctgg agtacctcgt atctcgacga agccgagcag
    12541 tgccgtgacg tgctgctgat gaacgaaggc gagttgctgt atcaggggga accaaaagcc
    12601 ctgacacaaa ccatggccgg acgcagcttt ctgatgacca gtccacacga gggcaaccgc
    12661 aaactattgc aacgcgcctt gaaactgcca caggtcagcg acggcatgat tcaggggaaa
    12721 tcggtacgtc tgatcctcaa aaaagaggcc acaccagacg atattcgcca tgccgacggg
    12781 atgccggaaa tcaatatcaa cgaaactaca ccgcgctttg aagatgcgtt tattgatttg
    12841 ctgggcggtg ccggaacctc agaatcgccg ctgggtgcaa tattgcatac ggtagacggc
    12901 acacccggcg agacggtgat cgaagcgaaa gaactgacca agaaatttgg tgattttgcc
    12961 gccaccgatc acgtcaactt tgccgttaaa cgtggggaga tttttggttt gctggggcca
    13021 aacggcgcgg gtaaatcgac cacctttaag atgatgtgcg gtttgctggt gccgacctct
    13081 ggtcaggcgc tggtgctggg gatggatctg aaagagagtt ccggtaaagc acgccagcat
    13141 ctcggctata tggcgcaaaa attttcgctc tacggcaacc tgacggtcga acagaattta
    13201 cgctttttct ctggtgtgta tggcttacgc ggtcgggcgc agaacgaaaa aatctcccgc
    13261 atgagcgagg cattcggcct gaaaagtatc gcctcccacg ccaccgatga actgccatta
    13321 ggttttaaac agcggctggc gctggcctgt tcgctgatgc atgaaccgga cattctgttt
    13381 ctcgacgaac cgacgtccgg cgttgatccc ctcacccgcc gtgaattttg gctgcacatc
    13441 aacagcatgg tagagaaagg cgtcacggtg atggtcacca cccactttat ggatgaagcg
    13501 gaatattgcg accgcatcgg cctggtgtac cgtgggaaat taatcgccag cggtacgccg
    13561 gacgatttga aagcacagtc ggctaacgat gagcaacccg atcccaccat ggagcaagcc
    13621 tttattcagt tgatccacga ctgggataag gagcatagca atgagtaacc cgatcctttc
    13681 ctggcgtcgc gtgcgggcgc tgtgcgttaa agagacgcgg cagatcgttc gcgatccgag
    13741 tagctggctg attgcggtag tgatcccact gctactgctg tttatttttg gttacggcat
    13801 taacctcgac tccagcaagc tgcgggtcgg gattttactg gaacagcgta gcgaagcggc
    13861 gctggatttc acccacacta tgaccggttc gccctacatc gacgccacca tcagcgataa
    13921 ccgtcaggaa ctgatcgcca aaatgcaggc ggggaaaatt cgcggtctgg tggttattcc
    13981 ggtggatttt gcggaacaga tggagcgcgc caacgccacc gcaccgattc aggtgatcac
    14041 cgacggcagt gagccgaata ccgctaactt tgtgcagggg tatgtcgaag ggatctggca
    14101 gatctggcaa atgcagcgag cggaggacaa cgggcagact tttgaaccgc ttattgatgt
    14161 acaaacccgc tactggttta acccggcggc gattagccag cacttcatta tccccggtgc
    14221 ggtgaccatc atcatgacgg tcatcggcgc gattctcacc tcgctggtgg tggcgcgaga
    14281 atgggaacgc ggcaccatgg aggctctgct ctctacggag attacccgca cggaactgct
    14341 gctgtgtaag ctgatccctt attactttct cgggatgctg gcgatgttgc tgtgtatgct
    14401 ggtgtcagtg tttattctcg gcgtgccgta tcgcggttcg ctgctgattc tgttttttat
    14461 ctccagcctg tttttactca gtaccctggg gatggggctg ctgatttcca cgattacccg
    14521 caaccagttc aatgccgctc aggtcgctct gaacgcggct tttctgccgt cgattatgct
    14581 ttccggcttt atttttcaga tcgacagtat gcccgcggtg atccgcgcgg tgacgtacat
    14641 tattcccgct cgttatttcg tcagcaccct gcaaagcctg ttcctcgccg ggaatattcc
    14701 ggtggtgctg gtggtaaacg tgctgttttt gatcgcttcg gcggtgatgt ttatcggcct
    14761 gacgtggctg aaaaccaaac gtcggctgga ttagggagaa gagcatgttt catcgcttat
    14821 ggacgttaat ccgcaaagag ttgcagtcgt tgctgcgcga accgcaaacc cgcgcgattc
    14881 tgattttacc tgtgcttatt caggtgatcc tgttcccgtt cgccgccacg ctggaagtga
    14941 ctaacgccac catcgccatc tacgatgaag ataacggcga gcattcggtg gagctgaccc
    15001 aacgttttgc ccgcgccagc gcctttactc atgtgctgct gctgaaaagc ccacaggaga
    15061 tccgcccaac catcgacaca caaaaggcgt tactcctggt gcgtttcccg gctgacttct
    15121 cgcgcaaact ggataccttc cagaccgcgc cgttgcagtt gatcctcgac gggcgtaact
    15181 ccaacagtgc gcaaattgcc gccaactacc tgcaacagat cgtcaaaaat tatcagcagg
    15241 agctgctgga aggaaaaccg aaacctaata acagcgagct ggtggtacgc aactggtata
    15301 acccgaatct cgactacaaa tggtttgtgg tgccgtcgct gatcgccatg atcaccacta
    15361 ttggcgtaat gatcgtcact tcactttccg tcgcccgcga acgtgaacaa ggtacgctcg
    15421 atcagctact ggtttcgccg ctcaccacct ggcagatctt catcggcaaa gccgtaccgg
    15481 cgttaattgt cgccaccttc caggccacca ttgtgctggc gattggtatc tgggcgtatc
    15541 aaatcccctt cgccggatcg ctggcgctgt tctactttac gatggtgatt tatggtttat
    15601 cgctggtggg attcggtctg ttgatttcat cactctgttc aacacaacag caggcgttta
    15661 tcggcgtgtt tgtttttatg atgcccgcca ttctcctttc cggttacgtt tctccggtgg
    15721 aaaacatgcc agtatggctg caaaacctga cgtggattaa ccctattcgc cactttacgg
    15781 acattaccaa gcagatttat ttgaaggatg cgagtctgga tattgtgtgg aatagtttgt
    15841 ggccgctact ggtgataacg gccacgacag ggtcagcggc gtacgcgatg tttagacgta
    15901 aggtgatgta acttcttatc tttcgccagc aaagacacta ccgccgggcc ggcaaggatt
    15961 gccagccctg caattgccag caaaaagttg ttttccagta cacgggacaa cagccacagc
    16021 acaatcagcg ctgcgccaaa ataccaggtc gtggtcagct cttccagcac gtctgccacc
    16081 tcacgccagc gttgctcggg ggggcgctga aacaccaaca tcatcactac cgtgacacaa
    16141 tacagtgcgc ataatcccac gccgacgtga accagagtct tactcatcca gcccaccacc
    16201 agcagcgcca ctaccagtaa atgcaacata atctgccagc aacttagacc agttgcgaca
    16261 cgaacacgtt gttgccactt catggcttct ctcctgaaga atttatctct acttactcag
    16321 agtaccgaac tttacggaaa attccgtaag accgcacctt tttgtgacgc cgactacact
    16381 atttcaatga gataagaggc aaagacagga aaattatgcc cgatcaaaca caacaatttt
    16441 cgttcaaggt gctcaccatc aacattcaca aaggttttac cgcgtttaac cgacgcttca
    16501 ttttgccgga acttcgcgac gcggtgcgta ccgtcagcgc cgatattgtt tgcctgcagg
    16561 aagtgatggg cgcgcacgaa gttcatccgc tgcatgtgga aaactggccc gatacctcgc
    16621 actacgagtt tctcgccgac acgatgtgga gcgattttgc ctacggtcgc aatgccgtat
    16681 acccggaagg gcatcacggc aacgccgtac tgtcgcgtta tcccattgaa cattatgaga
    16741 atcgcgatgt ttcggtcgat ggtgcggaaa agcgcggcgt gctctactgc cgcattgtgc
    16801 caccgatgac cggaaaagcg attcatgtga tgtgcgtaca tctgggcctg cgtgaggcgc
    16861 accgtcaggc gcagcttgcg atgctcgccg aatgggtgaa tgagctaccg gacggcgaac
    16921 cggtattggt ggcgggtgat ttcaatgact ggcggcaaaa agctaatcat ccgttaaaag
    16981 tgcaggccgg actggatgag atttttaccc gcgcccacgg acgcccggcg cgcacgtttc
    17041 cggtgcaatt tcctctacta cgactggaca ggatctacgt caaaaatgcc agcgccagcg
    17101 cgccaaccgc gttgccgctg cggacatggc gacacctttc tgatcatgcc cctttaagtg
    17161 cggagattca tttatgaaat gtagctggcg cgaaggcaat aagatccagt tgctggaaaa
    17221 cggcgagcaa tattatcccg cggtgtttaa ggcgattggc gaggcacaag aacgcatcat
    17281 tcttgaaacg tttatctggt ttgaggatga cgtcggcaaa cagctgcatg cggcactact
    17341 ggcagcagcg caacgcgggg ttaaagcgga agtcttgctg gatggctacg gttcgccgga
    17401 tctcagcgat gagtttgtca atgaactgac ggcagctggc gtggtatttc gctactacga
    17461 tccccgccct cgcctttttg gtatgcgcac caatgtgttt cgccggatgc atcgcaaaat
    17521 tgtggtgatc gacgcgcgta tcgcctttat tggcgggctg aattactccg ccgagcatat
    17581 gtccagctac ggtccagagg ctaaacagga ttacgcggta cgccttgaag ggccgattgt
    17641 cgaagatatt ctccagtttg agctggaaaa cctgcctgga cagagcgcgg cacgacgctg
    17701 gtggcgacgt catcacaaag cggaagaaaa tcgccagccg ggagaagcgc aggtattgct
    17761 ggtctggcgc gataacgaag aacaccgcga tgatatagaa cgccactatc tgaaaatgct
    17821 cactcaggcg cggcgggaag tcattatcgc caacgcctac ttcttccccg gctatcgatt
    17881 tttacacgcc ttgcgtaaag cggcacgccg cggggtgcgg atcaaactga tcattcaggg
    17941 cgaaccggat atgccgattg tcagagtcgg cgcgcgcttg ctgtataact atctggttaa
    18001 aggcggcgtt caggtgtttg agtaccgccg ccgcccgctc cacggcaaag tagcattgat
    18061 ggacgatcac tgggcaacag tagggtccag taatctcgat ccgctcagtt tgtcactgaa
    18121 tctcgaagca aatgtcatca tccacgatcg taattttaac cagacgctgc gcgataatct
    18181 gaacggcatt atcgccgcag attgtcagca ggtggatgaa accatgctgc ccaaacgcac
    18241 ctggtggaac ctgaccaaaa gcgtgctggc gttccacttt ttacgccact tcccggcgct
    18301 ggttggctgg cttccggcac acacgccacg tctggcgcag gttgatccgc ccgcacaacc
    18361 gacaatggaa acgcaggatc gggtagaaac tgaaaacacg ggggtaaaac cctgatgagt
    18421 aaatcacacc cgcgctggcg cttagcaaag aagatcctca cctggctgtt ttttatcgcg
    18481 gtgattgtgt tgctggtggt ctacgccaaa aaagtggact gggaagaggt ctggaaggtc
    18541 atccgcgact acaatcgcgt tgcgctgctt agtgcggtcg ggctggtggt cgtcagctat
    18601 ctgatttacg gctgctatga cctgctcgcc cgcttttact gcggtcacaa actggcgaag
    18661 cgccaggtga tgctggtgtc gtttatctgc tacgccttca acctgacgct cagtacctgg
    18721 gtcggcggca ttggtatgcg ctatcgtttg tactctcgtc tggggttgcc cggtagcact
    18781 attacgcgga ttttctcgct cagtattacc accaactggc tgggctatat tttactggca
    18841 gggattatct ttaccgcagg cgtggtggag ttgccggatc actggtatgt cgatcaaact
    18901 acgctgcgta ttctcggcat tggcttactg atgattatcg cggtttattt gtggttttgc
    18961 gctttcgcga agcaccgcca tatgaccatc aaagggcaaa agctggtgct gccttcatgg
    19021 aaattcgccc tcgctcagat gctgatttcc agcgttaact ggatggtaat gggggcgatt
    19081 atctggctgt tacttggtca aagcgtgaac tatttctttg tactgggcgt gttactggtt
    19141 agtagtattg ctggcgtcat cgtgcatatt ccggcgggga tcggtgtgct ggaagcggtg
    19201 tttatcgcac tactggctgg ggagcatacc tcgaaaggga gcattatcgc cgccctgctc
    19261 gcttatcgcg tcctgtatta ctttatcccg ctgctgctgg cgctgatctg ttacctgcta
    19321 ctggaaagcc aggcgaagaa gctacgggcg aaaaatgaag cggcgatgtg aagtttagtt
    19381 aagttcttta gtatctgcat ttacgactaa tgaaaaaaac gcgtatgcct ttgccagaca
    19441 agcgttatag ctatcgcggc aagtatccca aagaagccga taaaatagta atagagtgtt
    19501 aatgcacctg taacagcgca acgaaccgga ctccacaact cttcactatg gcatttcccc
    19561 gccaattcaa ggagcttatt tcgtccatgc gagataatgc ctgaccataa cacaatcatt
    19621 aagcaactta ttacccaaac gggtcgttca ctcattaatg cggcattcat gatgatcacc
    19681 agggccaacc ctgtcaacgt catcataatg ataaaacggt gagaaccagg atcaacatta
    19741 aataataaac cggccagcat actgattaaa aacatcgcgc ccgcagtgct gaacacgacg
    19801 ataatggcag gtacaggagc taaacttgca acgacaatcg ccaccaaaga gagtaaaaac
    19861 agatatatta cgcctgcgat gacggcattt atcttgttga tatccttact taagccatac
    19921 atacaaacta ataataaccc tatggcgaca tacattgttg gtgaatcata tttggttgcc
    19981 gtaatttcga gatacttagc tccccaggcc agtcctgcgg ttgccaccaa acctaaagcg
    20041 gtaatgagcc atataccgtt taatatcctg gtctcgtgct gaaaatctaa tttattgctg
    20101 ttttgtgagt atgattccat tcttttagtt gtccttagaa aaaatattaa tattcaccat
    20161 caataaattt aaaaccacgc atacaaaata cttttgtatt tatggatacg gaaaacgctt
    20221 ccgctcgcca taaggcgtat tttatacctg gtacttgtta agtgtcgttt ttagtaagaa
    20281 aaaaaatccc gacacatggc cgggatattg atggtgaaaa agaattaacg gcggttgcca
    20341 aaaattcgca gcaacatcag gaacaggttg atgaagtcca gatacaaggt taacgcgcca
    20401 agaatggaat atttgcgcag gttcgacgtg tcgcgggtat caatctgctc acccatattt
    20461 ttcaatttct gagtgtcata cgccgtcaat ccgacaaaga caatcacgcc gatgtaggta
    20521 actgcccaca tcaatgcttc gcttttcagc cagaagttga ccagcgatgc cagcacaatg
    20581 ccgattaacg ccataaacag catattgcca aagccactta aatcgcgctt cgtggtgtaa
    20641 ccgtacaggc tcattgcgcc gaacatcccg gcagtaacga cgaaagtact ggcgatagaa
    20701 gcagcggtat agacaatgaa tatactggaa agcgtaagac ccgtcagcgc cgaataaagc
    20761 ataaagagca tcgtcgttac acctgcgctc agcttttgaa tcatcgctga taacacaata
    20821 accaatgcta attgcgcgat gatcagaccg attaaaaaga cacggttagt aaacaacagc
    20881 tccatcacgg ccgcggaatt agccgcatac caggcaacaa atgcggtcag caacaagcca
    20941 acggtcatcc agccatagac ttgcgccata taagtttgca agccagcccg gggttgtacg
    21001 attgaatcag aacgtgggaa tctgtccatg acgatctcct gaagatataa ggaatatctt
    21061 aaggatactg caaaatgatg aggctgtgca tcgacgcagc gtaaacgcat gtactgagcg
    21121 gtgaaattgc cggacgcagc ggtgccttat ccggctaaca aaaaactacc agcgttttgc
    21181 cgcctgctga tcgctctccc gagcttcaac ccagcggtcg ccttccggcg tggcttcgcg
    21241 cttccagaac ggtgcgcggg ttttgagata atccataata aactgcccgg cttcaaacgc
    21301 actgctgcga tgcgcactgg tgacaccgac aaaaacgatt tcatcgcccg gccataattc
    21361 cccgatgcgg tgaatcacag tgacgcgccc cagcggccag cggttacgcg cttcatcaac
    21421 aatttctgcc agtgcttttt cagtcatccc cggatagtgt tcgagggtta atgctttgac
    21481 gctgtcgccc aggttatggt tgcgcacctt accagtaaag gtgactaccg caccgtcttc
    21541 gtcacgctcc gccagccacg ggtactcttc tcctacgctg aacggctgcg gaccaacaac
    21601 aattttggtt tctgccatct taacctccgg ttaccggcgg gaagaaagct acttcgtcgc
    21661 cgtcagtcag cggatggtca aaactcacca gcgtctggtt gacggcagcc agtaatttgc
    21721 catcttccag cgccagcgcc cagcgatcgc tctgcgcagc catgtgctgg cgtaacgctt
    21781 caacagttgg gaaatccgca gccacttcgg ttgcatctgt tcccaccaac tcgcgcacct
    21841 gggcgaaaaa aagaacttta atcatccgct tccaccttaa agtcacccga cttgccgccg
    21901 ctcttcgcca gcaaacgtac cggaccaatc accatatctt tttgcaccgc tttgcacatg
    21961 tcataaatgg tcagcgccgc cacggaggcc gcggttaatg cttccatttc gacaccggtt
    22021 ttcccggtca ggcggcataa ggtttctata cgcacccgat tgtgctccgg ctcggcctgt
    22081 aaattgactt caactttgct gagcatcagc ggatgacaga gcgggatcag atcccaggtg
    22141 cgttttgccg cctgaatacc ggcaatacgc gcagtggcaa atacgtcgcc tttgtggtgg
    22201 cgaccatcaa taatcatcgc cagcgtctcg ctgcgcatgg tgacaaaggc ttccgcccgc
    22261 gcttcacgca cggtttccgc tttggcggag acatccacca tgtgcgcttc gccagcggcg
    22321 ttgatatggg tcagttgcga catacttatt tcttcaaatg tggatggaaa ttacacggac
    22381 gcgtacgggc atccagctgc ggcgcgatga tattttccca tgcggtacgg cacgctttgg
    22441 tcgaacccgg catggcgaaa atcagcgttt tgttggcgac gcccgctacc gcacgagatt
    22501 gcaacgtgga agtgccaatc tcttcaaacg acaacatacg gaacacttca ccaaaacctt
    22561 caatttcacg gtcgaacaac ggcagcaatg cttcgggagc ctgatcacct tccgtcaggc
    22621 cagtaccacc cgtaatcaat accacttgta catcgtcgct ggcgatccac gcagatacct
    22681 gagcgcgaat agcgtagcgg ttttctttca caatggcttt atcgacaacg tgatggcccg
    22741 cttcttgcgc cgaatcgcgc agatagtgac cggaggtatc gtcttcttca ccgcgacgat
    22801 tagaaaccgt aagaatagca atacgggtcg ggataaattc agtgcttacc tgactcatct
    22861 gatctctcct tttgacgttt tagccgccaa tgtacgataa gttttgcgta ataccggtgt
    22921 tgttttgatg caggaaatgg gtctgtttct tctcccgcag cgccgctgaa atacgcgctt
    22981 ccagcgcctg ttgctgggta tcgtcttcca gcagatcgcg caggttaacg ccgccttcac
    23041 caaacaggca gagatggagt ttaccaatgg aggaaacgcg caggcggttg caagtggcgc
    23101 agaagtcttt ttcatacggc atgataaggc caatctctcc ggcgtaatct ggatggcaaa
    23161 agacttgcgc gggaccgtcg ctgcgttgac gtaattggtg gatccagccg cgacgcagta
    23221 gctcgtcacg cagaacctga ccagagatgt gatgctta
//
LOCUS       DAMZET010000059        22885 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.059, whole genome shotgun sequence.
ACCESSION   DAMZET010000059 DAMZET010000000
VERSION     DAMZET010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.059"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            4..657
                     /gene="rluA"
                     /locus_tag="POB34_002371"
     CDS             4..657
                     /gene="rluA"
                     /locus_tag="POB34_002371"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HDI5919307.1"
                     /translation="MENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKDSV
                     MTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWGHP
                     SPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGRSH
                     QLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPADF"
     gene            complement(774..1589)
                     /gene="djlA"
                     /locus_tag="POB34_002372"
     CDS             complement(774..1589)
                     /gene="djlA"
                     /locus_tag="POB34_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HDI5919308.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQTGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            1844..4198
                     /gene="lptD"
                     /locus_tag="POB34_002373"
     CDS             1844..4198
                     /gene="lptD"
                     /locus_tag="POB34_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HDI5919309.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNSL"
     gene            4251..5537
                     /gene="surA"
                     /locus_tag="POB34_002374"
     CDS             4251..5537
                     /gene="surA"
                     /locus_tag="POB34_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HDI5919310.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATADIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            5537..6526
                     /gene="pdxA"
                     /locus_tag="POB34_002375"
     CDS             5537..6526
                     /gene="pdxA"
                     /locus_tag="POB34_002375"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HDI5919311.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRTYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPLLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            6523..7344
                     /gene="rsmA"
                     /locus_tag="POB34_002376"
                     /pseudo
     CDS             6523..7344
                     /gene="rsmA"
                     /locus_tag="POB34_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
     gene            7347..7724
                     /gene="apaG"
                     /locus_tag="POB34_002377"
     CDS             7347..7724
                     /gene="apaG"
                     /locus_tag="POB34_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HDI5919312.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            7731..8573
                     /gene="apaH"
                     /locus_tag="POB34_002378"
     CDS             7731..8573
                     /gene="apaH"
                     /locus_tag="POB34_002378"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HDI5919313.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(8651..9130)
                     /gene="folA"
                     /locus_tag="POB34_002379"
     CDS             complement(8651..9130)
                     /gene="folA"
                     /locus_tag="POB34_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HDI5919314.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(9322..11184)
                     /gene="kefC"
                     /locus_tag="POB34_002380"
     CDS             complement(9322..11184)
                     /gene="kefC"
                     /locus_tag="POB34_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HDI5919315.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(11177..11707)
                     /gene="kefF"
                     /locus_tag="POB34_002381"
     CDS             complement(11177..11707)
                     /gene="kefF"
                     /locus_tag="POB34_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HDI5919316.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(11815..13146)
                     /locus_tag="POB34_002382"
     CDS             complement(11815..13146)
                     /locus_tag="POB34_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5919317.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWRSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(13204..13491)
                     /gene="fixX"
                     /locus_tag="POB34_002383"
     CDS             complement(13204..13491)
                     /gene="fixX"
                     /locus_tag="POB34_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HDI5919318.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(13488..14774)
                     /locus_tag="POB34_002384"
     CDS             complement(13488..14774)
                     /locus_tag="POB34_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDI5919319.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(14825..15766)
                     /locus_tag="POB34_002385"
     CDS             complement(14825..15766)
                     /locus_tag="POB34_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDI5919320.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(15781..16551)
                     /locus_tag="POB34_002386"
     CDS             complement(15781..16551)
                     /locus_tag="POB34_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HDI5919321.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            17025..18539
                     /gene="caiT"
                     /locus_tag="POB34_002387"
     CDS             17025..18539
                     /gene="caiT"
                     /locus_tag="POB34_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HDI5919322.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            18570..19712
                     /gene="caiA"
                     /locus_tag="POB34_002388"
     CDS             18570..19712
                     /gene="caiA"
                     /locus_tag="POB34_002388"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HDI5919323.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            19841..21058
                     /gene="caiB"
                     /locus_tag="POB34_002389"
                     /pseudo
     CDS             19841..21058
                     /gene="caiB"
                     /locus_tag="POB34_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308068.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
     gene            21132..22685
                     /gene="caiC"
                     /locus_tag="POB34_002390"
     CDS             21132..22685
                     /gene="caiC"
                     /locus_tag="POB34_002390"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HDI5919324.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLLTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            22794..>22885
                     /locus_tag="POB34_002391"
     CDS             22794..>22885
                     /locus_tag="POB34_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="catalyzes the dehydration of L-carnitinyl-CoA to
                     crotonobetainyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitinyl-CoA dehydratase"
                     /protein_id="HDI5919325.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKT"
BASE COUNT         5612 a         6176 c         5884 g         5213 t
ORIGIN      
        1 gggatggaaa actacaatcc accgcaggaa ccctggttgg ttatcctgta tcaggatgac
       61 catattatgg tggtcaacaa gccgagcggt ttgttgtcag tgccgggtcg tctggaagag
      121 cacaaagaca gcgtgatgac gcgcattcag cgtgattatc cgcaggcaga atcggtgcat
      181 cgtctggata tggctaccag cggcgtgatt gtagtggcgc tgaccaaagc cgcggagcgg
      241 gagttaaaac gccagttccg cgagcgcgag ccgaaaaagc agtatgtggc ccgcgtctgg
      301 gggcatccat cccccgcaga aggtctggtg gatctgccgc tgatttgcga ctggccaaac
      361 cgcccgaaac agaaagtctg ttacgaaacg ggtaaacctg cgcagacgga atatgaagtg
      421 gtggagtatg cggcggataa cacggcaaga gtggtgttaa aaccgattac cgggcgttcg
      481 catcaattgc gtgtgcatat gctggcgctg gggcatccga ttctcggcga tcgtttttat
      541 gcatcaccag aagcgagagc gatggcaccg cgtttgttgc tacatgcaga gatgttgacg
      601 attacccatc cggcgtatgg caatagtatg acgtttaaag cgccagcgga tttttaatcg
      661 tcacgctggc gcagttcggt aggcggataa ggcgttcacg ccgcatccgg caattgtgcg
      721 ccgattgcct gatgcgacgc gggccgcgtc ttatcaggcc tacagtttaa aacttattta
      781 aaccctttct gctgctttat cagctcatat gcctgctgaa tttcctgcgc tttctgcttc
      841 gccatctcca tcatctcagg cggcaaacct ttcgccacca gcttatcggg atggtgttca
      901 ctcatcagct tacggtaggc acgtttgatg gtggtcgcat catccgtcgg cttcacgccc
      961 agcacattac aggcatcttc cagcgttggg ccacgctgcg cttgctgcca gttaccaccg
     1021 ccagtttgct gctgataacc gccgccaaac tgtgcaccgc cctgcatcat gcgcaaaaac
     1081 tggtcaaact gagcgcggga gatccctaat tcttctgcaa tgacatacag caccgcccgt
     1141 tcattcgggt gcagtgaacc atcagcaaac gccgcctgaa tctggatctc cagaaacata
     1201 cgaattaagt caaaacgacc aaagcagaca ctgcgaaact ggcgcatctt ttcgcgcagc
     1261 gggtaattgt ctgattttcc cacccggaac gcattttgcg ccgcagtacg ggaagcgcca
     1321 tgaagattca ttcggtccat caactggctg gcgatatgaa tatcagcctc cgtgacgcga
     1381 cctttggatt tggttaaatg ccccatcact tcaaaagtgg tggcaaaaaa cagcgcctga
     1441 cgctcacgct ggttggcgaa ccacgccatt ttacggctac gggctttatc aaacatatgg
     1501 ccaattaaca ggcctaacac tacgccccaa aagccgccgc ccatcagtaa ggccacggcc
     1561 acgccaatga tttttcccca atactgcata tattccccaa atcgacacac ggatatcagg
     1621 gctatctccc acaatataaa ggtgctttta ccgttttccg gctgcggtca attgtgggac
     1681 atcgcctata atttgcatta tcatacctgt cattcaatgc cgtgcctaac accactgacg
     1741 ctattcggac aggattaaca ctagcgtaga gatgacgagt acgttagtct ctgaccgttt
     1801 gtcacgcgca acgttaccga tgatggaaca ataaaatcaa cgtatgaaaa aacgtatccc
     1861 cactctcctg gccaccatga ttgccaccgc cctttatagt caacagggac tggcagccga
     1921 cctcgcctca cagtgcatgt tgggcgtgcc aagctatgac cgtcctctgg tacagggcga
     1981 taccaatgac ttacccgtga ctatcaatgc tgaccacgcg aaaggggact acccggatga
     2041 cgccgtgttt actggcagcg tggatatcat gcagggtaac agccgtctgc aggccgacga
     2101 agtgcagctc catcaaaaag aggcaccagg acaaccggag ccggtacgta ccgttgatgc
     2161 gctcggtaat gtccattacg acgataacca ggtgatcctc aaagggccga aaggctgggc
     2221 gaatctgaac accaaagata ccaacgtctg ggaaggtgat taccagatgg tgggtcgcca
     2281 gggtcgcggt aaagcggacc tgatgaaaca acgtggcgaa aaccgctata ccattctgga
     2341 taacggtagc tttacctcct gtctgccggg ttctgacacc tggagcgtgg taggtagcga
     2401 aattattcat gaccgcgaag aacaagttgc ggagatctgg aacgcccgct ttaaggtggg
     2461 tccggtaccg atcttttata gcccctattt gcagttgccg gtgggtgaca aacgtcgctc
     2521 tggtttcttg atcccgaacg ccaagtacac caccaccaac tactttgagt tctacctgcc
     2581 atattactgg aacatcgcgc caaatatgga tgccaccatc acgccgcatt atatgcatcg
     2641 tcgtggcaac atcatgtggg agaacgaatt ccgctacctc tcccaggcgg gcgctggctt
     2701 gatggaactg gactatctgc cttcagataa agtctatgaa gatgaacacc cgaacgatga
     2761 cagttcacgt cgttggttat tctactggaa ccactccggg gtcatggatc aggtgtggcg
     2821 tttcaacgtc gactacacca aggtcagcga tcctagctac ttcaatgatt tcgataacaa
     2881 gtacggttcc agtactgacg gctacgcaac gcaaaaattc agcgttggct atgcggtgca
     2941 aaacttcaat gccaccgttt caaccaagca gttccaggtt ttcagcgaac agaacaccag
     3001 tagctactcg gcagagccgc agttagacgt taattactac cagaatgatg ttggtccgtt
     3061 tgatacgcgt atttacggcc aggcagtgca ctttgttaac accagagacg acatgcctga
     3121 agcaacccgt gttcacctgg aaccgaccat caatttgccg ctctctaata actggggcag
     3181 catcaatacc gaagcgaagt tgctggcaac ccattatcag caaactaatc ttgactggta
     3241 taactccaga aacacgacca agctggacga atccgttaac cgcgtaatgc cgcaattcaa
     3301 agttgacggc aaaatggtct ttgaacgcga tatggaaatg ctggctccgg gttataccca
     3361 aacgctggaa ccgcgcgcgc agtatttgta cgtgccgtat cgcgatcaga gcgacatcta
     3421 taactacgac tcatctctgc tgcaatctga ctactctggc ctgttccggg accggactta
     3481 cggcggtctt gaccgtattg cctccgctaa ccaggtgacg accggtgtca catctcgcat
     3541 atatgatgat gctgccgttg aacgttttaa tatttccgtt ggtcaaatct actatttcac
     3601 ggagtctcgc actggcgatg acaacataac atgggagaat gacgacaaaa cgggctcact
     3661 ggtgtgggca ggcgatactt actggcgtat ctccgagcgt tggggattgc gtggcgggat
     3721 tcagtacgat acacgtctgg ataacgtagc gaccagtaac tccagcattg aataccgtcg
     3781 ggatgaagac cgtctggtac agctgaatta ccgttacgcc agcccggaat atattcaggc
     3841 tacgctgcct aagtactatt ccactgctga gcaatataag aatggtattt cgcaggtagg
     3901 tgctgtcgcc agctggccaa ttgccgatcg ttggtccatt gttggggcct actactacga
     3961 caccaatgcg aacaagcaag ctgactctat gttaggtgtg caatacagct cctgctgcta
     4021 tgcaattcgc gtcggttacg agcggaagct gaacggttgg gataacgata aacaacatgc
     4081 ggtatatgac aacgcaatcg gctttaacat cgaacttcgc ggcctgagct ccaactacgg
     4141 tctgggtacg caagagatgc tgcgttcgaa cattctgccg tatcaaaact ctttgtgatt
     4201 tgttgattta ccacgtaatc cgcagtgcgg ttaattgaaa tggaaaaagt atgaagaact
     4261 ggaaaacgct gcttctcggt atcgccatga tcgcgaatac cagtttcgct gccccccagg
     4321 tagtcgataa agtcgcagcc gtcgtcaata acggcgtcgt gctggaaagc gacgttgatg
     4381 gattaatgca gtcggtaaaa ctgaacgctg ctcaggcaag gcagcaactt cctgatgacg
     4441 cgacgctgcg ccaccaaata atggaacgtt tgatcatgga tcaaatcatc ctgcagatgg
     4501 ggcagaaaat gggagtgaaa atctccgatg agcagctgga tcaggcgatt gctaacatcg
     4561 cgaaacagaa caacatgacg ctggatcaga tgcgcagccg tctggcttac gatggtctga
     4621 actacaacac ctatcgtaac cagatccgca aagagatgat tatctctgaa gtgcgtaaca
     4681 acgaggtgcg tcgtcgcatc accatcctgc cgcaggaagt cgaatccctg gcgcagcagg
     4741 tgggtaacca aaacgacgcc agcactgagc tgaacctgag ccacatcctg atcccgctgc
     4801 cggaaaaccc gacctctgat caggtgaacg aagcggaaag ccaggcgcgc gccattgtcg
     4861 atcaggcgcg taacggcgct gatttcggta agctggcgat tgctcattct gccgaccagc
     4921 aggcgctgaa cggcggccag atgggctggg gccgtattca ggagttgccc gggatcttcg
     4981 cccaggcatt aagcaccgcg aagaaaggcg acattgtggg tccgattcgt tccggcgttg
     5041 gcttccatat tctgaaagtt aacgacctgc gcggcgaaag caaaaatatc tcggtgaccg
     5101 aagttcatgc tcgccatatt ctgctgaaac cgtcgccgat catgactgac gaacaggccc
     5161 gcgtgaaact ggaacagatt gctgctgata tcaagagtgg taaaacgact tttgctgccg
     5221 cagcgaaaga gttctcgcag gatccaggtt ctgctaacca gggcggcgat ctcggttggg
     5281 caacagcgga catttttgat ccggctttcc gtgacgccct gacccgcctg aacaaaggtc
     5341 aaatgagtgc accggttcac tcttcattcg gctggcattt aatcgaactg ctggataccc
     5401 gtaatgtcga taaaaccgac gctgcgcaga aagatcgtgc ataccgcatg ctgatgaacc
     5461 gtaagttctc ggaagaagca gcaagctgga tgcaggaaca acgtgccagc gcctacgtta
     5521 aaatcctgag caactaatgg ttaaaaccca acgtgttgtg atcactcccg gcgagcccgc
     5581 cgggattggc ccggacttag ttgtccagct tgcacagcgt gagtggccgg tcgaactggt
     5641 tgtttgtgcc gatgccactc tccttaccga ccgggcagcg atgctcggtt tgccgctcac
     5701 cctccgcact tattccccca actctcctgc acaaccgcaa actgcgggca cattaacgct
     5761 acttcctgtc gcgctacgtg aatctgtcac tgcggggcag ttagcggttg aaaatgggca
     5821 ttatgtggtg gaaacgctgg cgcgagcgtg cgatggctgt ctgaacggtg aatttgctgc
     5881 gctgatcaca ggccccgtgc ataaaggcgt cattaacgac gcaggcattc cgtttaccgg
     5941 tcataccgag tttttcgaag agcgttcgca ggcgaaaaaa gtggtgatga tgctggcgac
     6001 cgaagaactt cgcgtggcgc tggcaacgac gcatttaccg ctgcgcgata tcgcagatgc
     6061 tatcacccct gcgcttttgc acgaagtgat tgctattttg catcacgatt tgcggaccaa
     6121 atttggtatt gccgaaccgc gcattctggt ctgcgggctg aatccacacg cgggcgaagg
     6181 cggtcatatg ggtacggaag agatagacac cattattccg ctgctcgacg agttgcgggc
     6241 gcaggggatg aaacttaacg ggccgctgcc tgccgatacc ctgtttcagc cgaaatatct
     6301 cgataacgcc gacgccgtgc tggcgatgta ccacgatcag ggtcttcccg tgctaaaata
     6361 ccagggcttc gggcgcggtg tgaacattac gctgggcctg ccctttattc gcacatcagt
     6421 ggaccacggc accgcgcttg aactggcggg acgtggcgaa gccgatgtcg gcagttttat
     6481 tacggcgctt aatctcgcca tcaaaatgat tgttaacacc caatgaataa tcgagtccac
     6541 cagggccact tagcccgtaa acgcttcggg caaaactttc tcaacgatca gttcgtgatc
     6601 gacagcattg tttctgccat taacccgcaa aagggccagg caatggtcga aatcggcccc
     6661 ggtctggcgg cattgaccga gccggtcggc gaacgtctgg accagctgac ggtcatcgaa
     6721 cttgaccgcg atctggcggc acgtctgcaa acgcatccat tcttaggccc gaaactgacg
     6781 atttatcagc aggatgcgat gacctttaac tttggcgaac tggccgagaa aatgggtcag
     6841 ccgctgcgtg ttttcggcaa cctgccttat aacatctcca cgccgttgat gttccatctg
     6901 tttagctata ctgatgccat tgccgacatg cactttatgt tgcaaaaaga ggtggtgaat
     6961 cgtctggttg caggaccgaa cagcaaagcg tatggtcgat taagcgtcat ggcgcaatac
     7021 tattgcaacg tgatcccagt gctggaagta ccgccgtcag cctttacacc accacccaaa
     7081 gtggattccg ccgtcgtgcg cctggttcct catgcaacga tgcctcaccc ggttaaagat
     7141 gttcgtgtgt tgagccgcat caccaccgaa gcctttaacc agcgtcgtaa aaccattcgt
     7201 aacagcctcg gcaacctgtt tagcgtcgag gtgttaacgt gaatggggat cgacccggcg
     7261 atgcgagcgg aaaatatctc tgtcgcgcaa tattgccaga tggcgaacta tctggcggag
     7321 aacgcgcctt tgcaggagag ttaacgatga tcaattcgcc ccgagtgtgt attcaggttc
     7381 aaagcgtcta cattgaggca caatcttcac ctgataatga acgttacgtt tttgcttata
     7441 ccgtaaccat acgcaatctg gggcgagcgc cagtgcagtt gttggggcgt tactggctga
     7501 tcaccaatgg caatggccgt gaaaccgaag tccagggcga aggagtggtt ggcgtccagc
     7561 cacttatcgc gcctggcgaa gagtaccagt acaccagcgg tgcaatcatt gaaaccccgc
     7621 tgggcaccat gcagggtcac tacgaaatga tcgatgaaaa tggcgtccct ttcagcatcg
     7681 acattcccgt attccgactc gccgttccca cactcattca ttaaaagaat atggcgacat
     7741 accttattgg cgacgttcat ggttgttacg atgaactgat cgcattgctg cataaagtag
     7801 aatttacccc tgggaaagat accctctggc taacgggcga tctggtcgcg cgcgggccgg
     7861 gttcgctgga tgttctgcgc tatgtgaaat ccttaggcga cagcgtacgt ctggtgctgg
     7921 gcaatcacga tctgcatctg ctggcggtat ttgccgggat cagccgcaat aaaccgaaag
     7981 atcgcctgac accgctgctg gaagcgccgg atgccgacga gctgcttaac tggctgcgtc
     8041 gccagccttt gctgcaaatc gacgaagaga aaaagttggt gatggcccac gcagggatca
     8101 cgccgcagtg ggatctacag accgccaaag agtgcgcgcg cgatgtagaa gcggtgctat
     8161 cgagtgactc ctatcccttc tttctcgacg ctatgtacgg cgatatgcca aataactggt
     8221 caccggaatt gcgggggctg ggaagactgc gttttatcac caacgctttt acccgtatgc
     8281 gtttttgctt cccgaacggt caactggata tgtacagcaa agaatcgccg gaagaggccc
     8341 ctgccccact gaaaccgtgg tttgcgattc ctggccctgt cgctgaagaa tacagcatcg
     8401 cctttggtca ctgggcatcg ctggagggca aaggtacgcc ggaaggtata tacgcgctgg
     8461 ataccggctg ctgctggggt ggtacattaa cctgcctgcg ctgggaagat aaacagtatt
     8521 ttgtccagcc gtcgaaccgg cataaggatt tgggcgaagc ggcggcgtct taaacacagc
     8581 ctgatatagg aaggccggat aagacgcgac cggcgtcgca tccggcacta gccgtaaatt
     8641 ctatacaaaa ttaccgccgc tccagaatct caaagcaata gctgtgagag ttctgcgcat
     8701 cagcatcgtg gaattcgctg aataccgatt cccagtcatc cggctcgtaa tccgggaaat
     8761 gggtgtcgcc ttccacttct gcgtcgatat gcgtcagata cagtttttgc gcttttggca
     8821 agaactgttc ataaacgcga ccgccgccaa tcaccatgat ttctggtacg tcaccacacg
     8881 ccgcgatggc ttcatccacc gacttcaccc acgttacgcg atcgtccgta cccggttgac
     8941 tgctgaggat aatatttttg cgtcctggca acggacgacc gattgattcc caggtatggc
     9001 ggcccataat cacgggttta tttaaggtgt tgcgtttaaa ccaggcgaga tcggcaggca
     9061 ggttccacgg catggcgttt tccatgccga taacgcgatc taccgctaac gccgcaatca
     9121 gactgatcat tgagatttcc cgataaaaaa aattgtcgcc actatacgta aagcgtaaac
     9181 cgtcgtcgac tggtgcgagg atgatgttga ggaaaatttt atattctgct ggcgagtcca
     9241 cgctctctcc gtggactcgc cgcattacaa tgaaacaaaa acaaacagtt agctgtaaag
     9301 tgtgatttac gtcactcttt attaggatga gggtttcgtt tccggttcat ccgccatgtt
     9361 gccggtatgt ttaccttctt cggttccctg ccagccatgt cgttgaatta atgacagatg
     9421 ttcgcggtcc tcggtaatga tctcacttaa catcgcgctg gtgcgtttat agaccgccgc
     9481 gcgggctttg gtgtcgttct caaccattgc catctcttcc accatctgaa tattaaagcg
     9541 gcggaacaca tcggcacgtt ctcgcgcttc atacggcccc agacctaaac tttccagtgc
     9601 cagacgcccg gttttcagcg caccttcgaa ggtttcacgc tccggctttt caacgcctgc
     9661 ctgacgcaaa cgaatgtagt ggtcgacatc gcgggcgcgg gcaataatct gcaaatgcgg
     9721 gaaatgttct ttcaccatct cagtcagttg caggttggtt tgcggatcgt cgatggcgtt
     9781 aatcagcact tccgctttcg ccgctccggc agattccagt aaatccatcc gcgtggcatc
     9841 gccataaaac actttcatac caaatttacg caaggtttcg atatggtccg gatcgtgatc
     9901 gagtaccacc attttcaccc cgctggagag cagtaaacgt ccggtaatct gcccaaaacg
     9961 accgaatccg gcgataatca cgcgcggctg ttcttcgtcg atctcatcgg cttcacgcgc
    10021 ttcctcagta gaagattgct caaggcgatt gaggatcacc agcagaatcg gcgttgctgc
    10081 catcgacagc gccaccgcca gggtcagcga tttcgcccac tccggctcca gcacattcgc
    10141 catctgcgcc gcgccaaata ccacaaaggc aaactcactg ccctgcccta acaacaccgc
    10201 aaaccaacga cgctgtttat ttggcacttg caacggtcgg gcaatcagcc acagcatggc
    10261 gattttgatg atgaggaaac cgagcagcaa aatgacaatg cgcaatgggt tttcaagcag
    10321 cgtgccaaag tctatcgaca tgccaacacc gatgaaaaac agccccaaca gcaaaccttt
    10381 aaatggttcg atatcgctct ccagcgcatg acggtattcc gagcttgcca gcagtacgcc
    10441 cgccagaaac gcgcccatcg ccatcgacaa gccgacctct tccagcagca aaccaaagcc
    10501 aaacacgagg aataacgcca cggcactaaa cacttcccgc aagccagagc gggctacaaa
    10561 acgcagcgcc ggacgcgtga catagcgccc cagcaatacc accagcacca gcgcacccgc
    10621 cacttttaac gccgagagag caaatgcgcc catcgtcgtc gaggcactgc tcgttgccag
    10681 tagcggaatc atcgccacca gcgggatcgc cgcgatatcc tggaacagca gcaccgcaaa
    10741 ggcactgcga cccatttgcg tcaccatcag attgcgttca ttcatcgcct gcatggcaat
    10801 cgccgtagag gagagcgcca gcgtcatgcc gatcaattcc gcgacctgcc agcgcagccc
    10861 aagtaacatg cagaacagcc ccagcaggcc accgcaaatc accatctgca atgcgccacc
    10921 gccgaacact gccgcacgca gcttccacag cctttgtgga tcgagttcga ggccgataat
    10981 aaacagcatc agcaccacgc caatctcggc aaagtgcaga atagattcgg catcggtcac
    11041 cagtcgcagc ccccacgggc caataatgca gccggcgatc aggtagccaa gtaccgatcc
    11101 cagaccaaga cgtaccgcaa tgggtacaat cagcgctgcc gaaccgagat aaatcagcgc
    11161 ctgaatcagc gtatggctat ccatgatggg cctcctgcca ttccagcaga cgttgcttat
    11221 agtgacgcgc ctgcccttcg agggtttcgt cgtcacaaat aaaggtgcag tgcatggcaa
    11281 acggtggcag ccagttcagc ccgcagtaga ttgccgtcgc ctgtagcggc tgcgacagca
    11341 catcaaagcc cggatgcgca ccaatttcaa aatggctttc cccgccgccg gtcgtcaccg
    11401 cccacagcaa atgtttgcca tgcagcgccg tgccgccgtg accgtaagcc cagccgtgcg
    11461 agaaaacttt atcgatccaa agtttgagga gcggtggaat gctgtaccac tgcatcgggt
    11521 gctgccagac gatcagatcg gcgcgagaca gcgcctcctg ctcggcggca atatcgatat
    11581 tgaagtcagg atagagttga taaagagagc gaatttcgac gccttccagc gtccttgcct
    11641 gttcaagcat ccgtttattc gcatgggaat gatgcggata cggatgcgca taaattataa
    11701 gaatcatagg tagcccgcgt gattacgcct ttgttttccc acaaagtgta gtcatatatt
    11761 cacgcggctt atagtcaata atattgattc agaaaagaga gaaatctgaa caaattaccc
    11821 cattctctgc ccgcggatcg tcatattgct ggtctgcgcc agtgacatcc ctcgagtctc
    11881 cggggcaaac gctacggaaa tcaacaagcc aaacagcgag atacccgccc ccattagcat
    11941 cgtgttactg ataccgtaat tattgataaa gatcggcagt gcccaggtcg aaacaatggt
    12001 gccaatacgg cttaaggaca taatcacgcc cacggcagag gcgcggatat ctgtcgggaa
    12061 gagttcatta ggatagagcc actgcaaatt acccggcccg ccagagaaaa aggcatacac
    12121 cgcaaaggcc atcactacca gccagatccc catatccggg atcagcccca aaaccgccag
    12181 cgccagcgtc atcatggcaa agctgccaat caacaatgga cgccgtccgg cagtgtttaa
    12241 ccacagcatc ggcggaatac agccgagcat aaagaacagg ctaatcacca cgttccccag
    12301 tgccgcgttt ttgccaaccc ccaatcccaa caaaccaacg atttgcgggc caaaggtgta
    12361 aatggcgaac attgggatca cctggcaggt ccagatggcg gcaacaaaca gaacaaaagg
    12421 aaaatggcgg cgattaaaca gatcgcgaaa acgggtttgc tgcggctgct cttcatcgaa
    12481 agccaccggt tcgccaaaca gttttatcat catctcttcg cactctttta ctcgcccttt
    12541 gcgtaataac cagcggggag attcaggcag ttcgaatcga ccaatcaaaa tcaacaaaca
    12601 ggggatcgcc gcgctaccca gcatccagcg ccagccgcct tccacatcat aaagccagta
    12661 gccgaccaga tcggcgcagg tcgcgccgac ataccacatg gcggcgataa agctgatgga
    12721 aaacgcccgc tgacgggtac tggagaactc ggtgatcatt gaggtggcga tgggataatc
    12781 tgcaccgatg acaatgccga taagtacccg catcaccaac agttcgacag gggatgagac
    12841 aaacatcgtc gccaccgata tcacgccgat ggcgatgata tcaatgagga acattttgcg
    12901 ccgtccgact ttatcggaga tatagccaaa cagcgatgtg ccaacgaaca gcccggcgag
    12961 cgttcccgca cccagcaagc caatccagtc agcgtccagt ttcagcgccg gagtcagttg
    13021 ctccagcgcc acgccaatca ttaccagtac ataaccatcc agaaacggac caccgcttct
    13081 ccacagcaaa atgcggcggt gaatagagga gaatttgaga tcgtcaaagt ttctggacgg
    13141 ttgcatgggt tattcctgtt ttttgcaatg agagtcagtg cggcgttacg tatcaaacca
    13201 acatcagccg taacggaact ccacaccaaa ggtgccgcgc gggtattccc actgttccag
    13261 cgccgacccc agccccagaa tgcgacaggt gccgcactcc agacatccgg cgtaatcgaa
    13321 gcgcacactg ccgtcatcct gcttcttgta cagacctgcg gggcacgctt tcaccagcag
    13381 ctccagcacc tgtttatcag catcggcctt cacaacaatg tgcggatgct cttcatcgac
    13441 attgaattta ttgacgccca gtttgacgtc cacattgacg ggagaagtca taaaacggtc
    13501 actcctttca tgccatcctt gattagattg atgaagccca ctttcttacc gtggcggagg
    13561 attttcttgc gcatcagttc tggcgcactg ccgtcaatgg tgaacaggtc acgcgccacg
    13621 cccaccgcca gctccgggta gccgctgaac atgcgtgggt tatcaaggaa cgccggtagt
    13681 ttctggtaca tgcgcatatc gcgcagcggg ccactctcaa gatgctgacg atattccgcc
    13741 agtttttgct tactgaaatc gtcgcttttc atcgctgaaa gcacggtttt tgctgcagct
    13801 tccccggcgg caatcgccag atccataccg cgaatggtaa aaccgaggtt catacacatt
    13861 ccggcggcat cgccggcaat caatacgccg tcaccgacca gctccggcag catgttgatg
    13921 cctgcttccg gcactacgtg agcggaatat tccaccagct tgccacccgc gatcagcggt
    13981 gcaacggccg gatgttgttt gaaatcttcc agcatttgcg gcaccgattt ttttgcgtca
    14041 tgcagatgat gcagaccaca aaccagcccc agcgacaggg tattttcatt ggtataaagg
    14101 aagccgccgc ccatcaggcc atcggtgggt gatcccgcaa acagacaagc cgcgccctga
    14161 ttaccctgca actgaaaacg gtcttcgatt accgacttcg gtaactcgat cagttccttc
    14221 acgccaaccg ccacatccgt cggtttaacg cgttttgcca tccccagctt ttcggcaagg
    14281 atggagttca ctccatcagc aaggatcacc gttttcgctt caatcacatc gccatcggct
    14341 tctacaccga cgactttgcc atcgcgctgt acgaggttat cgacgcggat cccggtaatt
    14401 aactgcgcgc ccgcttcttc ggcctgctcc atcagccagg catcaaattt actgcgcaaa
    14461 acggagtaag aacgctggga tggcgaggtt tcgtcaccat tgcagtagtc catagtcatc
    14521 gctgactttt ccgtcataaa cgcgagtttt tcatgggtga tcaggcgttc tacgggggcg
    14581 gagtcggcga aaccaggaat aatgtgttct aggctgtggg catagagacg cccgccggtg
    14641 acgttcttgg cacctgcgga attgccacgc tcgataacta acacttgcgc cccttcgcgg
    14701 gcgagcacca gtgcggcaac cgaaccggca agcccagcac cgacgatgat ggcgtcaaag
    14761 atatcttcgg acataagagc tccctgtcag cggcagggcc aaaatgcagc cctgccagag
    14821 tggatcaacg cgctaaagct gcggtcagcg ccggaaggat cttcacggcg tcaccaacaa
    14881 tgccgtaatc cgcgtactgg aagatcggcg catttttatc tttattgatg gcgaaaatgg
    14941 tttgcgatgc attagcgcca accatgtgct ggatctgccc ggagatcccc accgccaggt
    15001 acagttcagg tttcagcatc aggttggaga taccgacata gcgttcgtgc tccatccatt
    15061 tttcgttttc cgccaccgga cgagaacagg ccaactccgc acctatcgcc ttgcaaagct
    15121 gttctgccag cgcaatgttc tctttgctgc caataccgcg accgacgctg accaccagac
    15181 gggctttgtc gagatcgacg ctgttgctct ggcgcgcctg ggttgccgtg cgggtaatcg
    15241 ccacagccgg agcctgccac tccacggtgt gcgtttcgcc agtgcgtgac gcgtctggct
    15301 gagccgcatc gaacgtgccg ctgctgatgg tcagtaccgc atacggcgtg gcaatgcgtt
    15361 cttcgccaat cgccagacca ccgtaaacca tgtgtttcac tgtcgcttta ccgtcctgta
    15421 cgctgacggt gctggcatcg ttagacaccg ccgctttaag gcgataaccc agttttgccg
    15481 ccagtaattt gccgcgacgg gtgtttggca gcagcaccag gccgtctgcg ccgtgctggc
    15541 gaatagtgtc agccatgaca ccggcgtaat cttcgatcat ccgatcgtcc ggtttgccgt
    15601 ttaatttcca gacatgatta gcgccgagct ggattgcctg tgcgccgtcg gcatcattga
    15661 ggacaaaggt gttgatttga ttagctaaag cctgcgcacc gttcatcagt tccggcagac
    15721 gagaaggggt atcgctgaat acccagactt gagaaaacgt gttcatagca tcccctgtaa
    15781 ttaaatgact ttgcgaagat tttcagcaaa tgcggcgatc tgttcttcgc cgtcgccttc
    15841 aatcacgatg cgctgacgtt cgcgctgttt cggcgcggca acctgttgtt ctgaccaggc
    15901 tgcctctgcg ttaaaaccaa tatccgccgc cgaccatacc tggacgggct ttttcgccgc
    15961 gccgagaatg gctttcatcg aaggaatttg tggggaattg atatcagtgg aaacagcaac
    16021 aaccgcaggc agcggaatgc ttaaggtttc ggtttcatct tccagttcgc gctcaacggt
    16081 gagggtatct gcagtcaggg agataatttt gctgacgccg ttaactgccg gaatattgag
    16141 gatttcgccc accagcagac caacctgctg ggcataaagg tcggaagaac catcgccaca
    16201 gaggatcaaa tcaaagcctg ctttctgggc ggctgcagcc agtgcgctcg ccgtttgttg
    16261 cggcagtgcc tgctcgaact ggtcatcaat caccacaatc agttcatccg ggccgcgcga
    16321 tagcacatct ttacgccctt tggcgttggt cagggcttta ccgcccacac ttaaggccgt
    16381 cacctgcgcc tctgctgcct gttgctttag ctggcaagcc gcttcaatag cgttcagatc
    16441 gtattggctt attttggcat cggctttgct gaagtctaat gaaccatcag cattattgac
    16501 cgcaatatcc tgttcatcag gcacgcactt atagcaagta ataatcttca ttgcatctcc
    16561 agaaatcatg aaggaaacgt cgttcatatc ggtggtgtta attacagata tcacgccctt
    16621 taatttggaa ataaaacttt aatcaccaat attgaaaatg tcacacgcag ataaatccaa
    16681 ctttcaatat tgttaagttc ctcaccaata ttgaaaacat ggcgtagcaa aaagaaattt
    16741 tcaatattgc tttatggatc accaatattg aaagtcagat gctatttttt tactcacaac
    16801 acaacataac aaactgatta ataatcaaaa ttaacgagaa aagcgccctc tattcacccg
    16861 aaacaaaaat gtgataacaa tcacagaatg cagcttattg aatacccatt atgagttagc
    16921 cattaacgcg tccacgaggt taataataat tatattaaat gttaacaaaa ataaaacaaa
    16981 cgggaacgca aaataaatat tcgttttcac agtggaatta actcatgaag aatgaaaaga
    17041 gaaaaacggg aatagaaccg aaggttttct ttccgccgtt aataatcgtc ggcatacttt
    17101 gttggcttac agtcagagat ctggatgcag cgaatgtcgt tattaatgct gtattcagtt
    17161 acgtcaccaa tgtatgggga tgggcatttg aatggtatat ggtggtgatg cttttcggtt
    17221 ggttctggct ggtgtttggc ccgtatgcca aaaagcgttt aggtaacgaa ccacctgaat
    17281 ttagtaccgc cagttggatc tttatgatgt tcgcctcctg tacgtctgct gccgtactgt
    17341 tctggggatc gattgagatc tactactaca tctccacccc gccgtttggc ttagaaccga
    17401 actcgacagg agcgaaagag ttggggctgg cttacagctt gttccactgg ggacctctgc
    17461 cgtgggccac ttacagcttc ctttcagtcg ccttcgctta cttcttcttt gtccgcaaaa
    17521 tggaagtgat tcgccccagc tcgacactgg tgccgctggt aggtgaaaaa cacgccaaag
    17581 ggttgttcgg cactatcgtc gacaacttct atctcgtcgc cttgatcttc gcgatgggta
    17641 ccagtctggg ccttgccacg ccgctggtga ccgagtgtat gcaatggttg tttggcattc
    17701 cgcataccct gcaactggac gctatcatca ttacctgctg gattatcctc aacgccattt
    17761 gcgtcgcttg cggtctgcaa aaaggggtac gtatcgccag tgacgtgcgt agttacctga
    17821 gcttcctgat gctgggttgg gtgttcattg tcagcggtgc cagcttcatc atgaactact
    17881 tcaccgattc ggtggggatg ttgctgatgt atctgccgcg catgttgttc tataccgatc
    17941 ccatcgctaa aggcggcttc ccgcagggct ggaccgtgtt ctactgggca tggtgggtga
    18001 tttatgctat ccagatgagt atcttcctcg cccgcatctc ccgtggtcgt actgtgcgtg
    18061 aactgtgctt cggcatggtg ctggggctga cagcgtcaac ctggatcctg tggactgtac
    18121 tcggtagtaa cactctgctg ttgatagata aaaacatcat caacattcca aatctgatcg
    18181 aacagtacgg tgtggcgcgc gccatcattg aaacctgggc cgctctgcca ctcagcaccg
    18241 ccaccatgtg gggcttcttc atcctctgct ttattgccac cgttacgctg gttaacgcct
    18301 gctcttatac cctggcgatg tccacttgcc gcgaagtacg cgatggtgaa gaaccacctc
    18361 tgctggtgcg tatcggttgg tcaattctgg ttggcattat cggtattgtt ctgctggcgc
    18421 tcggcggcct gaaaccgatt caaaccgcca ttatcgccgg aggatgcccg ctgttcttcg
    18481 tcaacattat ggtgacgctc tcctttatta aagacgcgaa acagaactgg aaagattaat
    18541 taacccccaa aatatcaaga ggttgaaaga tggattttaa tttaaatgat gagcaggaac
    18601 tgtttgtcgc cggtatccgc gaactgatgg ccagcgaaaa ctgggaggcc tattttgccg
    18661 agtgcgaccg tgacagcgtc tacccggaac gttttgtcaa agcactggcg gatatgggta
    18721 tcgacagtct gctgatccct gaagagcacg gtggtctgga cgcggggttt gttactctcg
    18781 ccgccgtgtg gatggagctg ggacgtctgg gggcaccaac ctatgtgctg taccagttgc
    18841 cgggcgggtt caacaccttc ctgcgcgaag gcacacaaga gcagatcgac aagattatgg
    18901 ctttccgcgg caccggtaag cagatgtgga actcagcgat taccgaaccg ggcgcgggct
    18961 ccgacgtggg tagcctgaaa acgacttata cccgtagaaa tggtaagatt tatcttaatg
    19021 gtagtaagtg ttttattacc agcagcgcct acaccccgta catcgtggtg atggcgcgcg
    19081 acggggcttc tccggacaaa cctgtctaca ccgaatggtt tgttgatatg agcaaaccgg
    19141 gcatcaaagt gaccaaactt gaaaagctcg gtctgcgtat ggatagctgc tgtgaaatca
    19201 cctttgacga cgtggaactg gacgagaaag acatgttcgg tcgggaaggt aacggcttta
    19261 accgcgtcaa agaagagttc gatcatgaac gtttcctggt agctctcacc aactacggta
    19321 cggcgatgtg cgcctttgaa gatgcggcgc gctacgccaa ccagcgcgtg cagtttggcg
    19381 aggctattgg ccgtttccag ttgattcagg aaaaattcgc ccacatggcg atcaaattaa
    19441 actccatgaa aaacatgctg tatgaagcag cgtggaaagc agacaacggc accatcacct
    19501 ctggcgatgc agcgatgtgc aaatacttct gcgccaatgc ggcatttgaa gtggtggata
    19561 gcgcaatgca ggtgctgggc ggtgtcggga ttgcgggcaa ccaccgcatc agccgcttct
    19621 ggcgtgacct gcgtgtagac cgcgtctccg ggggatctga cgaaatgcag atcctgacgc
    19681 tgggtcgcgc ggtgctgaag caataccgct aagtccgtgc gctgcctgat gcgacgctaa
    19741 tgcgtcttat caggcctacg ggagcacgaa tgtaggccgg ataaagcgtt tacgccgcat
    19801 ccggcagtca tgcgctacat aaatttttca ggagctaata atggatcatc tacccatgcc
    19861 gaaattcggg ccgttggccg gattgcgcgt tgtcttctcc ggtatcgaaa tcgccgggcc
    19921 gttcgccggg caaatgttcg cagaatgggg tgcggaagtt atctggatcg agaacgtcgc
    19981 ctgggccgac accattcgcg ttcaaccgaa ctacccgcag ctctcccgcc gcaatttgca
    20041 cgcgctgtcg ttaaatattt tcaaagatga aggccgcgaa gcgtttctga aattaatgga
    20101 aaccaccgat atcttcatcg aagccagcaa aggtccggcc tttgcccgtc gtggcattac
    20161 cgatgaagta ctgtggcagc acaacccgaa actggttatc gctcacctgt ccggttttgg
    20221 tcagtacggc accgaggagt acaccaatct tccggcctat aacaccatcg cctaggcctt
    20281 cagtggttac ctgattcaga acggtgatgt tgaccagcca atgcctgcct tcccgtatac
    20341 cgccgattac ttttctggcc tgaccgccac cacggcggcg ctggcagcac tgcataaagc
    20401 gcgtgaaacc ggtaaaggcg aaagtatcga catcgccatg tatgaagtga tgctgcgtat
    20461 gggccagtac ttcatgatgg attacttcaa cggcggcgaa atgtgcccgc gcatgagcaa
    20521 aggtaaagat ccctactacg ccggttgcgg tctgtataaa tgtgccgacg gctacatcgt
    20581 gatggaactg gtgggcatta cccaaattga agagtgcttt aaagatattg gcctcgcaca
    20641 tctactcagt acgccggaaa tcccggaagg cactcagctt atccaccgta tcgaatgccc
    20701 ttacggccca ctggtagaag aaaaactcga tgcctggctg gcggcacata ccatcgcaga
    20761 agttaaagaa cgctttgccg aactgaatat cgcctgtgcc aaagtactga ccgtaccgga
    20821 actggaaagc aatccacagt atgttgcccg tgaatcaatc actcagtggc aaacgatgga
    20881 tggtcgcacc tgcaaagggc cgaacatcat gccgaaattc aaaaataacc ccggacaaat
    20941 ctggcgcgga atgccctcac atggcatgga cacggctgcc attttgaaaa atatcggcta
    21001 cagcgaaaac gacattcagg agttggtcag caaaggtctg gccaaagttg aggactaaac
    21061 gcgtggcgtc agtcggcttg cctgactagc gcccgcccaa taacaaacga acaaaaatgg
    21121 atagaggtgc aatggatatc attggcggac aacatctacg tcaaatgtgg gacgatctgg
    21181 cggacgttta cggtcataaa acggcgctga tttgtgaatc cagcggcgga gtcgttaacc
    21241 ggtatagtta tcttgagtta aatcaggaga ttaaccgcac ggcaaacctg ttttatacgc
    21301 tggggattcg caaaggcgac aaggttgcac tacatctcga caactgcccg gaatttatct
    21361 tttgctggtt cgggctggca aaaattggcg cgattatggt gccgattaac gcccgcctgt
    21421 tacgcgagga aagcgcgtgg atcctgcaaa atagccaggc gtgcctgctg gtgaccagtg
    21481 cgcaattcta tcctatgtat caacagattc agcaggaaga tgccactcaa ttgcggcaca
    21541 tttgcctgac agatgtggca cttcccgctg atgatggcgt gagttcgttt actcaactga
    21601 aaaatcaaca acctgccacc ttgtgctatg caccgccgct attgactgac gatacggcgg
    21661 aaattctctt cacctccggc accacctccc gaccgaaagg tgtggtgatt acccattaca
    21721 acctgcgctt cgctggatat tactccgcct ggcagtgtgc actgcgtgac gatgacgtct
    21781 acctgacggt aatgcctgcg tttcatatcg attgccagtg tactgcggcg atggcggcgt
    21841 tttctgccgg ggccaccttt gtgctggtcg agaaatacag cgcccgcgcc ttctggggac
    21901 aggtgcagaa gtaccgcgcc accattaccg aatgtattcc gatgatgatc cgtacgttga
    21961 tggtgcagcc gccttcagcg aacgatcggc aacaccgcct gcgggaagtg atgttttatc
    22021 tcaacttgtc ggagcaggaa aaagatgcgt tttgtgaacg cttcggcgtt cgcttgctga
    22081 cgtcttatgg gatgacggaa accattgtgg gcattatcgg cgatcgccct ggcgataaac
    22141 gacgctggcc gtcgattggt cgggcggggt tttgctacga agcggagatc cgcgacgatc
    22201 acaatcgccc gctcccggca ggtgagatcg gtgaaatctg tattaaaggc gtaccaggga
    22261 aaaccatctt caaagagtat tttctcaacc cgaaagccac tgcgaaagtg ctggaagccg
    22321 acggctggct acataccggc gataccggat actgcgacga agagggcttt ttttatttcg
    22381 tcgatcgccg ctgcaatatg atcaaacgcg gtggcgagaa tgtctcctgc gtggagctgg
    22441 aaaatatcat cgccacccat ccaaaaattc aggatatcgt ggttgtgggt attaaagatt
    22501 cgattcgcga tgaagccatc aaagcatttg tggtgctgaa tgaaggtgaa acattgagcg
    22561 aagaggaatt tttccgcttc tgcgaacaaa atatggcgaa atttaaagtg ccctcttatc
    22621 tggagatcag aaaagatctg ccacgtaatt gctcggggaa aataattaga aagaatctga
    22681 aataaatgaa acagcaggga actaccctgc cagctaataa tcacgctata aaacaatatg
    22741 cattttttgc agggatgttg tcgtccctga aaaagcaaaa atggagaaaa ggaatgagtg
    22801 aatcattaca tctgacccgc aatggatcaa ttctggaaat tacccttgat cgtccaaaag
    22861 cgaatgctat tgatgcaaaa accag
//
LOCUS       DAMZET010000060        22733 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.060, whole genome shotgun sequence.
ACCESSION   DAMZET010000060 DAMZET010000000
VERSION     DAMZET010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.060"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1357)
                     /locus_tag="POB34_002392"
     CDS             complement(<1..1357)
                     /locus_tag="POB34_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI5919326.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KE"
     gene            complement(1487..2218)
                     /gene="pgeF"
                     /locus_tag="POB34_002393"
     CDS             complement(1487..2218)
                     /gene="pgeF"
                     /locus_tag="POB34_002393"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HDI5919327.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAADAKASTAFIQHGDKYLADIYLLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(2215..3195)
                     /gene="rluD"
                     /locus_tag="POB34_002394"
     CDS             complement(2215..3195)
                     /gene="rluD"
                     /locus_tag="POB34_002394"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HDI5919328.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIIINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            3330..4067
                     /gene="bamD"
                     /locus_tag="POB34_002395"
     CDS             3330..4067
                     /gene="bamD"
                     /locus_tag="POB34_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HDI5919329.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            4338..4679
                     /gene="raiA"
                     /locus_tag="POB34_002396"
     CDS             4338..4679
                     /gene="raiA"
                     /locus_tag="POB34_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDI5919330.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    4782..4907
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4783..4830
                     /gene="pheL"
                     /locus_tag="POB34_002397"
     CDS             4783..4830
                     /gene="pheL"
                     /locus_tag="POB34_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HDI5919331.1"
                     /translation="MKHTPFFFAFFFIFP"
     gene            4929..6089
                     /gene="pheA"
                     /locus_tag="POB34_002398"
     CDS             4929..6089
                     /gene="pheA"
                     /locus_tag="POB34_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HDI5919332.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTCLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(6132..7253)
                     /gene="tyrA"
                     /locus_tag="POB34_002399"
     CDS             complement(6132..7253)
                     /gene="tyrA"
                     /locus_tag="POB34_002399"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HDI5919333.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(7264..8334)
                     /gene="aroF"
                     /locus_tag="POB34_002400"
     CDS             complement(7264..8334)
                     /gene="aroF"
                     /locus_tag="POB34_002400"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HDI5919334.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            8544..8909
                     /locus_tag="POB34_002401"
     CDS             8544..8909
                     /locus_tag="POB34_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HDI5919335.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            9059..9577
                     /locus_tag="POB34_002402"
     CDS             9059..9577
                     /locus_tag="POB34_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HDI5919336.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            9567..10793
                     /gene="dgcN"
                     /locus_tag="POB34_002403"
     CDS             9567..10793
                     /gene="dgcN"
                     /locus_tag="POB34_002403"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HDI5919337.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISITSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            10809..11291
                     /locus_tag="POB34_002404"
     CDS             10809..11291
                     /locus_tag="POB34_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDI5919338.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(11367..11714)
                     /gene="rplS"
                     /locus_tag="POB34_002405"
     CDS             complement(11367..11714)
                     /gene="rplS"
                     /locus_tag="POB34_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDI5919339.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(11756..12523)
                     /gene="trmD"
                     /locus_tag="POB34_002406"
     CDS             complement(11756..12523)
                     /gene="trmD"
                     /locus_tag="POB34_002406"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDI5919340.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(12554..13102)
                     /gene="rimM"
                     /locus_tag="POB34_002407"
     CDS             complement(12554..13102)
                     /gene="rimM"
                     /locus_tag="POB34_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDI5919341.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(13121..13369)
                     /gene="rpsP"
                     /locus_tag="POB34_002408"
     CDS             complement(13121..13369)
                     /gene="rpsP"
                     /locus_tag="POB34_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDI5919342.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(13506..14867)
                     /gene="ffh"
                     /locus_tag="POB34_002409"
     CDS             complement(13506..14867)
                     /gene="ffh"
                     /locus_tag="POB34_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDI5919343.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            15034..15825
                     /locus_tag="POB34_002410"
     CDS             15034..15825
                     /locus_tag="POB34_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HDI5919344.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            15846..17132
                     /locus_tag="POB34_002411"
     CDS             15846..17132
                     /locus_tag="POB34_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDI5919345.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(17187..17780)
                     /gene="grpE"
                     /locus_tag="POB34_002412"
     CDS             complement(17187..17780)
                     /gene="grpE"
                     /locus_tag="POB34_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDI5919346.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            17903..18781
                     /gene="nadK"
                     /locus_tag="POB34_002413"
     CDS             17903..18781
                     /gene="nadK"
                     /locus_tag="POB34_002413"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HDI5919347.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            18867..20528
                     /gene="recN"
                     /locus_tag="POB34_002414"
     CDS             18867..20528
                     /gene="recN"
                     /locus_tag="POB34_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDI5919348.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            20677..21018
                     /gene="bamE"
                     /locus_tag="POB34_002415"
     CDS             20677..21018
                     /gene="bamE"
                     /locus_tag="POB34_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HDI5919349.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(21080..21370)
                     /locus_tag="POB34_002416"
     CDS             complement(21080..21370)
                     /locus_tag="POB34_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HDI5919350.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(21360..21797)
                     /gene="ratA"
                     /locus_tag="POB34_002417"
     CDS             complement(21360..21797)
                     /gene="ratA"
                     /locus_tag="POB34_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HDI5919351.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            21968..22450
                     /gene="smpB"
                     /locus_tag="POB34_002418"
     CDS             21968..22450
                     /gene="smpB"
                     /locus_tag="POB34_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDI5919352.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
BASE COUNT         5764 a         6008 c         5362 g         5599 t
ORIGIN      
        1 gttctttgtc gctcagttct tcgttgagca tatccaggcg ttttttactg gcttcatcag
       61 actctttcat taacgcctgt tgttccagtt tgagctggat gatacgacga tcgagtcggt
      121 cgagttcttc tggttttgag tcaatctgca tacgaatgct ggatgctgct tcatcgatca
      181 ggtcgatggc tttatccggc agctgacggt cagcaatgta gcgatgagac aacgtcgccg
      241 ctgcaacaat tgccgggtca gtaatttgca catggtggtg caattcgtaa cgttctttca
      301 ggccacgcag aatcgcaatg gtatcttcaa cagaaggctc ggcaacaaac actttctgga
      361 aacgacgttc cagcgcagca tctttttcga tgtactggcg atattcgtca agcgtcgtgg
      421 cacctacgca gtgcaattca ccacgcgcca gcgccggttt cagcatgttt ccggcgtcca
      481 ttgcgccatc ggctttaccc gcgccgacca tggtatgtaa ttcgtcgata aacaggatga
      541 cgttgccttc ctgtttggca agatcgttaa gcacgccttt taaacgttct tcaaactcac
      601 cgcgatattt cgccccagcc accagcgcgc ccatatccag cgccagtacg cggcggcctt
      661 tcaatccttc cggcacttcg ccgttgataa tacgctgcgc cagaccttca acgatggcag
      721 ttttaccgac gccgggttca ccaatcagta ccgggttatt tttagtacga cgttgcagca
      781 cctgaatggt acggcgaatt tcttcatcac gaccaatcac cggatcgagt ttgccctgtt
      841 cggctcgttc ggtaaggtcg atggtatatt ttttcaaagc ctgacgttgg tcttcagcac
      901 cttgatcgtt cacgctttca cctccacgca tttgttcaat cgcttgagta atgttggcgg
      961 tggtcgcccc tgctgctttc aggatgtcgg ccagcgtgcc gcgagactca agtgccgcca
     1021 gaacgaacag ttctgacgag ataaagttat caccacgttt ttgcgccagc ttgtcgcaaa
     1081 gattaagaac gcgcaccaga tcctgtgatg gctggacatc accaccagta ccttcaacct
     1141 gcggtaaacg atttaatgcc tgattgatat ctgtgcgcaa ctggccagca tttatgccag
     1201 ctgatgttaa caaaggacta accgaacccc cttcctgatt cagcagggcg ctcattaaat
     1261 gaagtggttc gatgaattgg ttgtcgtgcc cgagtgcaag tgattgggca tcggcaagag
     1321 caagctggaa tttattagta agacgatcca gacgcataac tcctcccata acggatcaaa
     1381 gttgctactg gagattaaat gaggtcatcc ctcaattatt caaggttatt gaccagatta
     1441 atgtgaaaag aaaatcacgc gtaccggatc gtcttgattc tttaggttat atcagccaaa
     1501 tgaaacttgc catacgcccg gtggtcttgt cgcgacgata agagaagaaa gtctcatttt
     1561 ccgtatatgt acaacggtcg ccgccgaaaa tttgttcaac accaacgctc gccagacgct
     1621 gccgggcaag cagataaata tccgccagat acttatcacc atgctgaatg aaagctgtac
     1681 tcgctttggc gtctgctgcc ataaacgcct cgcgaacctc cgcccccact tcgaacgcgc
     1741 gtggaccaat tgccggccct aaccaggcga gaatattttc cggattatca gcaaaacagg
     1801 aaactgtctc ttccagcacg cctgcgcaca gtccacgcca gccagcatga gcggcggcga
     1861 cttccgttcc cgctcgattg caaaacagca cagggaggca gtcggcagtc atcactgcgc
     1921 aaaccgtgcc aggcgtattg ctataagagg catctgctcg ttttgaggca taaggttcgc
     1981 cagtaagctt aagcacatct ttgccgtgta cctgctcaag ccagaccggt ttagaaggca
     2041 aattgcccgc agcaaaaagt cgcttgcgat tctcctcaac gtgatccggg ttatcgccac
     2101 aatgggcacc gaggttgagc gagtcatacg ggggcaagct cacgccgccg atacgagtag
     2161 agctacaagc cgcaacaccc tttggcagcg gccactgcgg gacaatcagc ttactcataa
     2221 ccagtccact tcatccttat gttcttcgaa atcggcgcgc atcacctcaa tcagctccac
     2281 catatcttgt ggaataggcg catgccattc catttcgatg ccggagatcg ggtgataaag
     2341 acgcagcatg gttgcatgta gcgcctggcg gtcaaactta cgcagcgtgg agataaatgc
     2401 ttccgaagca ccttttggcg gacgcggacg gccaccataa accggatcgc ccaccagcgg
     2461 atgagtgata tgggccatat gcacgcggat ctggtgcgta cgtccagttt ccagacgcaa
     2521 ccgcagacgc gtgtgcacac ggaagtgttc catgatgcga tagtgagtca ccgctggttt
     2581 gcccatcgga tgcaccgcca tatgggtacg tttggtcggg tggcgactga ttggctcgtc
     2641 caccgtgcca cctgcggtca tatgaccaat cgccaccgct tcatactcac gagtaatttc
     2701 acgccgttgc aaagattcga ctaaacgcgt ctgagccgga acggtttttg ccacaaccat
     2761 caggccagtg gtgtctttat ccagacgatg gacgatgccc gcacgcggta catcggcaat
     2821 gggtggatag taatgaagca acgcattcag taccgtgcca tccgggttac ccgcgccagg
     2881 atgtaccacc aggtcgcgcg gtttattaat gataataatg tcttcatctt catagacgat
     2941 atccagcggg atatcctgcg gttcaaaacg cgcttcttct tcaatctcag cgttgatggc
     3001 aacctgctcg ccacccaata ctttttcttt cggcttatca caaactttgc cgttaaccag
     3061 cactcgctgg tcgaggatcc attcttttat tcgcgaacgt gaataatccg ggaacatttc
     3121 ggccaaagcc tgatctaagc gttgaccgag ttggttttcg gacaccgttg cagtgagctg
     3181 tactcgttgt gccatatata ctgcttcttc gtttatcgtt ggggttttac ggctttgccg
     3241 tttaatatag tgtgctattg tagctggtct taaccgggag caggaacaga gaatctcccg
     3301 tattacattt tgaggaaagt caaaacgtca tgacgcgcat gaaatatctg gtggcagccg
     3361 ccacactaag cctgtttttg gcgggttgct cggggtcaaa ggaagaagta cctgataatc
     3421 cgccaaatga aatttacgcg actgcacaac aaaagctgca ggacggtaac tggagacagg
     3481 caataacgca actggaagcg ttagataatc gctatccgtt tggtccgtat tcgcagcagg
     3541 tgcagctgga tctcatctac gcctactata aaaacgccga tttgccgtta gcacaggctg
     3601 ccatcgatcg ttttattcgc cttaacccga cccatccgaa tatcgattat gtcatgtaca
     3661 tgcgtggcct gaccaatatg gcgctggatg acagtgcgct gcaagggttc tttggcgtcg
     3721 atcgtagcga tcgcgatcct caacatgcac gagctgcgtt tagtgacttt tccaaactgg
     3781 tgcgcggcta tccgaacagt cagtacacca ccgatgccac caaacgtctg gtattcctga
     3841 aagatcgtct ggcgaaatat gaatactccg tggccgagta ctatacagaa cgtggcgcat
     3901 gggttgccgt cgttaaccgc gtagaaggca tgttgcgcga ctacccggat acccaggcta
     3961 cgcgtgatgc gctgccgctg atggaaaatg cataccgtca gatgcagatg aatgcgcaag
     4021 ctgaaaaagt agcgaaaatc atcgccgcaa acagcagcaa tacataacag aaacctgaaa
     4081 cacaaaacgg cagcccttga gctgccgttt ttttattctg tcagttgtga aactgaaccg
     4141 atttagtcgc tatcgatctc atcaaatatg gctcgctttg agatattcct caagtaaaaa
     4201 aacacctctt cctgcgattt ctcacaaaaa agattcgttg acaaaaagtg acaaaattat
     4261 gagattttca tcacacattt tgacatcagg aacggtatgc tgaattcacc aagacgggaa
     4321 gacaagaggt aaaatttatg acaatgaaca ttaccagcaa acaaatggaa attactccgg
     4381 ccatccgcca acatgtcgca gaccgtctcg ccaaactgga aaaatggcaa acacatctga
     4441 ttaatccaca tatcattctg tccaaagagc cacaagggtt tgttgctgac gccacaatca
     4501 atacacctaa cggcgttctg gttgccagtg gtaaacatga agatatgtac accgcaatta
     4561 acgaattgat caacaagctg gaacggcagc tcaataaact gcagcacaaa ggcgaagcac
     4621 gtcgtgccgc aacatcggtg aaagacgcca acttcgtcga agaagttgaa gaagagtagt
     4681 cctttatatt gagtgtatcg ccaacgcgcc ttcgggcgcg ttttttgttg acagcgtgaa
     4741 aacagtacgg gtactgtact aaagtcactt aaggaaacaa acatgaaaca cacaccgttt
     4801 ttcttcgcat tcttttttat cttcccctga aagggaggcg tttcgtcgtg tgaaacagaa
     4861 tgcgaagacg aacaataagg cctcccaaat cggggggcct tttttattga taacaaaaag
     4921 gcaacactat gacatcggaa aacccgttac tggcgctgcg agagaaaatc agcgcgctgg
     4981 atgaaaaatt attagcatta ctcgcagagc ggcgcgaact ggccgtcgag gtgggaaaag
     5041 ccaaactgct ctcgcatcgc ccggtacgtg atattgatcg tgaacgcgat ttactggaaa
     5101 gattaattac gctcggtaaa gcgcaccatc tggacgccca ttacattact cgcctgttcc
     5161 agctcatcat tgaagattcc gtattaactc agcaggcttt gctccaacaa catctcaata
     5221 aaattaatcc gcactcagca cgcatcgctt ttctcggccc caaaggctcc tattcacatc
     5281 ttgccgctcg tcagtacgct gcccgtcact ttgagcaatt cattgaaagt ggctgcgcca
     5341 aatttgccga tatttttaat caggtggaaa ccggccaggc cgactatgcc gtcgtaccga
     5401 ttgaaaatac cagctccggt gccataaacg acgtttacga tctgctgcaa catacctgct
     5461 tgtcgattgt gggcgaaatg acccttacca tcgaccactg cctgctggtt tccggcacca
     5521 cagatttatc taccatcaac acggtctaca gtcatccaca gccgttccag caatgcagca
     5581 aattccttaa tcgttatccg cactggaaga ttgaatatac cgaaagtacg tctgcggcaa
     5641 tggaaaaggt tgcacaggca aaatcaccgc atgttgctgc gttgggaagc gaagctggcg
     5701 gcactttgta cggtttgcag gtactggagc gtattgaagc gaatcagcga caaaacttca
     5761 cccgatttgt ggtattggcg cgtaaagcca ttaacgtgtc tgatcaggtt ccggcgaaaa
     5821 cgacgttgtt aatggcgacc gggcaacaag ccggtgcgct ggttgaagcg ttgctggtgc
     5881 tgcgcaacca caatctgatt atgacccgtc tggaatcacg cccgattcac ggtaatccat
     5941 gggaagagat gttttatctg gatattcagg ccaatcttga atcagcggaa atgcaaaaag
     6001 cattgaaaga gttaggggaa attacccgtt caatgaaggt attgggctgt tacccaagtg
     6061 agaacgtagt gcctgttgat ccaacctgat gaaaaggtgc cggatgatgt gaatcatccg
     6121 gcactggatt attactggcg gttgtcattc gcctgacgca ataacacgcg gctttcactc
     6181 tgaaaacgct gtgcgtaatc gccgaaccag tgctccacct tgcggaaact gtcaataaac
     6241 gcctgcttat cgccctgctc cagcaactca atcgcctcgc cgaaacgctt atagtaacgt
     6301 ttgattaacg ccagattacg ctctgacgac ataataatgt cggcataaag ctgcggatcc
     6361 tgagcaaaca gtcgcccgac catcgccagc tcaaggcggt aaatcggcga agagagcgcc
     6421 agaagttgct caagctgaac attttcttct gccagatgca gcccataagc aaaagtagca
     6481 aagtggcgca gagcctgaat aaacgccata ttctgatcgt gctcgacagc gctaatacga
     6541 tgcagccgag cgccccagac ctgaatttgc tccagaaacc attggtatgc ttccggctta
     6601 cgtccatcac accagaccac aacttgcttt gccaggctac cgctgtccgg gccgaacatc
     6661 gggtgtaacc ccagtaccgg gccatcgtgc gccgccagca tggcctgtaa tggtccattt
     6721 ttcactgatg ccagatcaac cagaatacaa tctttcggta aaggcggtaa tttgccgata
     6781 acttgctcag taacgtggat tggcacacta acaatcacca ttccggcatc ggcaacaata
     6841 tcagccgctc gatcccagtc atgttgctcc agaatccgca cctgataacc cgatagtgtc
     6901 agcatcttct cgaacaggcg tcccatctga ccgccaccgc cgacgataac caccggacgc
     6961 agtgacggac aaagtgtttt aaatcctttg tcgttttcac tggagtaaga ttcacgcatc
     7021 acccgacgca aaacatcctc aatcagatct ggcggtacac ccagagcttc cgcctctgcg
     7081 cgccgcgagg ccaacataga tgcctcgcgc tccggaacat aaataggcag tccaaagcgg
     7141 cttttcacct cgcccacttc agcaaccagt tccagacgct tcgctaataa attcagcagc
     7201 gctttatcga cttcatcaat ttgatcgcgt aatgcggtca attcagcaac cataataaac
     7261 ctcttaagcc acgcgagccg tcagctgccc gttcagatcc tgatgaattt cacgcagcaa
     7321 ggcatcggtc atttcccagc taatgcaggc atcggttacg gatacaccgt atttcatttc
     7381 actgcgcggt tgctcggaag actgattgcc ctcgtggata ttactttcga tcatcagacc
     7441 aataattgag cgattgccat ctttgatttg agcaaccacg gattctgcca ccgcaggctg
     7501 acggcgataa tctttattgg aattaccgtg gctgcaatct accatcagag acgggcgcag
     7561 tcccgcctgt tccatctctt tttcacattg cgcaacatcc gcagggctat agttcggcgc
     7621 tttaccaccg cgcaggatca catggccgtc cggattcccc tgagtttgta gcaacgcaac
     7681 ctgccctgcc tggttaatgc caacaaaacg gtgcggctgg gcggcggcgc gcatagcgtt
     7741 aattgctgtt gccagactgc cgtcggtgcc gtttttaaaa ccaaccggca tggaaagccc
     7801 ggaggccatt tcacggtgag tttgcgattc cgttgtacga gcaccaattg ctgaccagct
     7861 aaacagatcg cccaggtatt gcgggctatt cggatctaac gcttccgtcg ccagtggcag
     7921 tcccatattc accagctcaa gcagcaattt acgcgcgatc tgcagcccgg cttctacatc
     7981 aaaagagcca tccatatggg gatcgttaat taaccctttc cagccgacag tggtacgggg
     8041 tttttcaaaa tagacgcgca ttaccagata gaggctatcg ctgacctctg cggcaagggc
     8101 tttaaatcga cgagcatatt ccagagcagt ttccggatca tgaatggaac aaggaccaca
     8161 tactaccagc agacgaggat cgcgcccggc gataatatct gaaatggttt tacgcgagtc
     8221 agcaatctgg gcttcttgtt gcaggctcaa tggaaaagcg gccttcagtt gttccggagt
     8281 cattaaaacc tgttcgtcgg taatatgtac gttattcagc gcgtcttttt gcatgatggc
     8341 gatcctgttt atgctcgttt gcgatagttg atcctcagcg aggatgacgt aacgataaca
     8401 cataaagtaa agttttcaat ccatatttcg tacattttta tttacacagg caatttagtc
     8461 gcgatttcaa cccttacttc tgtatagata aatttacact ccctttgaaa acaatccgct
     8521 atgctttgaa aaaggagaaa gaaatgatga aaaagtttat cgcccccttg ttggctttac
     8581 tggttagcgg atgtcagatt gatccttata ctcacgcgcc aaccttgacc agcaccgact
     8641 ggtatgatgt tggtatggaa gatgcgatat cgggcagcgc cataaaagat gacgatgcat
     8701 ttagcgattc acaggcggat cgcggtctat accttaaagg atatgccgaa ggacaaaaga
     8761 aaacttgcca gaccgatttt acttatgccc gaggactttc cggtaaaagc tttcctgcca
     8821 gctgcaacaa tgttgaaaat gccagccaac tgcatgaagt atggcaaaaa ggggctgatg
     8881 aaaatgccag cacaatacga ttaaattaat taaaacccca caaataaatt aaacataaga
     8941 ttttactcat tgtgttttat ttctcacatt gatgacgatc gcatgaaaaa atgatatttt
     9001 aatgagcatt ctgaaaaaat atcgatgcat ttcgagcgaa gatggtgggg atccctgaat
     9061 gcgtttttct caccgactgt tcttacttct gattctcctg ctgacgggag caccaatact
     9121 cgctcaggag ccttctgacg ttgcgaaaaa cgtgcgtatg atggtttccg gtattgtcag
     9181 ttacacccgc tggcctgcgc tatcagggcc accaaagtta tgtatctttt catcttcacg
     9241 ttttagtacc gccctacaag agaatgccgc gacatctttg ccttatctgc ctgtcatcat
     9301 tcacactcaa caagaggcga tgatttcagg ctgcaatggt ttttattttg gcaacgagtc
     9361 accgacattc cagatggaat taaccgaaca atatccgtca aaggcgttat tattaattgc
     9421 cgaacaaaat accgagtgca ttattggtag cgccttttgt ctgatcatcc acaataatga
     9481 cgtcagattt gccgtaaacc tggatgcctt atcgcgtagc ggtgtaaagg tcaacccgga
     9541 tgtcttaatg ctcgcacgga agaaaaatga tggataacga taattctctt aataagcgcc
     9601 ccacgtttaa aagagcatta cgcaacatca gtatcaccag catatttatc actatgatgc
     9661 tgatctggtt gctgctttcc gtgacctcgg tgctgaccct gaaacagtac gcgcaaaaaa
     9721 acctggcact gacagcagca acaatgactt acagtctgga agcagctgtc gtttttgccg
     9781 atggccctgc agcaactgaa acactggcag cgctgggcca gcaagggcaa ttttcaactg
     9841 cagaagtacg tgataagcag caaaatattc tggcgtcctg gcattacacc cgtaaggatc
     9901 caggcgatac tttcagcaat ttcataagcc actggctctt ccccgccccc atcattcagc
     9961 cgattcgtca caatggtgaa accattggcg aagtacgctt aaccgctcgc gacagttcaa
    10021 tcagccattt tatctggttt tcgctcgccg tactgaccgg ttgtattctg ctggcatcag
    10081 gcatcgcaat taccctcacc cgccatttgc acaatggcct ggtggaagca ctgaaaaata
    10141 tcaccgatgt cgtacatgat gtgcgttcca accgcaattt ttcccgacga gtttcggaag
    10201 aacgtatcgc tgagtttcac cgcttcgctc tcgacttcaa cagtctgctg gatgaaatgg
    10261 aagagtggca gcttcgttta caggctaaaa atgcgcagct tctacgtacc gcgctacatg
    10321 acccattaac cgggctggct aaccgcgcag cgtttcgtag cggcatcaac acgttgatga
    10381 acaattccga tgcccgaaaa acgtcggcgt tactatttct tgatggcgat aatttcaaat
    10441 acatcaatga tacctggggt catgcgacgg gcgatagagt cttgattgaa atcgcaaaac
    10501 ggttagctga agttggcggg ctgcgacata aagcataccg cctgggcggc gatgaattcg
    10561 ctatggtgct ctatgatgta cagtcagaat ctgaagtgca gcagatatgc tcagcactga
    10621 cacaaatctt taatctcccg tttgatcttc ataatggtca tcagaccacc atgacattaa
    10681 gcattggtta cgcgatgacc attgagcacg cctctgcgga aaaattacaa gagcttgccg
    10741 atcacaatat gtatcaggcc aaacaccagc gtgccgaaaa gctggtgaga taacaaggat
    10801 atatcgatat gataaagcac ctggtagcac ccctgatttt cacctcacta atactgactg
    10861 gctgccagtc ccctcaggga aagtttactc ctgagcaagt cgccgctatg caatcttatg
    10921 gatttactga atccgccggc gactggtcgc tgggcttatc agatgccatt ctgttcgcaa
    10981 aaaatgacta caaattgctc ccggaaagcc agcaacagat ccaaaccatg gcagccaaat
    11041 tggcctcgac agggctaaca catgcccgta tggatggaca caccgataac tatggtgaag
    11101 acagttacaa cgaaggctta tcattgaaac gggcgaatgt cgtggccgat gcatgggcta
    11161 tgggtggaca aattccacgc agcaatctca ccacacaggg tttaggaaaa aaatatccca
    11221 tagccagtaa caagaccgcc cagggccgcg ccgagaaccg ccgcgtcgca gtggtgatta
    11281 ctacccctta aaacaccagc aaacagataa aaaagggcca gccaattggc cagcccttct
    11341 taacaggatg tcgcttaagc gaaatcttag ttaagacgct ctttgatacg agcagcctta
    11401 ccagtacgct cacgcaggta gtacagttta gctttacgaa cagcaccacg acgtttgaca
    11461 gaaatgctgt caactaccgg agagtgagtc tggaagacac gctcaacgcc ttcgccgttg
    11521 gaaattttac gaacagtgaa tgcagagtgc agaccgcggt tacgaatagc gataaccacg
    11581 ccctcgaatg cctgcagacg ttttttggaa ccttcaacaa cccatacttt cacttccacg
    11641 gtatcacccg gacggaagga aggtacgtcc tgcttcatct gctcttgttc aagttgctta
    11701 ataatgttgc tcataattta atctcttatc ctgggtaaac tgatatctcg ggggcttacg
    11761 ccatcccatc atgtttatgt tgctgttgtg cgtgttccgt tttgaactcc gccagcaacc
    11821 ttgcttgctc ttcagtcaga gccaggtttt ccagaagttc aggtcttcta agccaggtac
    11881 ggcccagcga ctgtttcaaa cgccagcgac gtatctcggc atggttgccc gacagtaaca
    11941 ctggcggaac ttccatccct tctaacacct caggccgcgt atagtgcggg caatccagta
    12001 atccttcagc aaaggaatct tccgttgccg aggcttcatg tcccagtact cccggaataa
    12061 accgggaaac ggagtcaatc agcgtcattg ctggtaactc accaccactg agaacgtaat
    12121 cgccgattga ccattcttcg tcaatttcgg tttggatcac gcgctcatct ataccttcgt
    12181 agcgaccgca caccagaatc aatttttgat tcgttgccag ttcgctgacg cccgcttgat
    12241 caagcttgcg tccctgtggt gacagataaa tcacctttgc gccttcaccc gccgcggctt
    12301 ttgctgcatg aatggcgtcc cgcaagggtt gcaccatcat taacatcccc ggtccgccgc
    12361 cgtaaggacg atcgtccacg gtacggtgcc ggtcatgcgt gaagtcgcga ggactccagc
    12421 tctggatgct cagcaggcca tttttaactg cccggccagt taccccgtaa tcggtaattg
    12481 cgcggaacat ttcaggaaac aggctaatta tgccaatcca catagcgccg tcttttaccg
    12541 tttatccggt ggtttaaaaa ccaggatccc aatctacttc gattgaacga gtagtgagat
    12601 cgactttctt gataacctgc ccatcgagga acggtacgag acgttccttg ataccaaacg
    12661 catctttcag gtttgcctta atgacgagaa cgtcattaga tccggtttcc atcatatcga
    12721 cgactttacc gagatcgtag ccttcagtgg ttactacctg gcagcccatc aggtctttcc
    12781 agtagtagtc cccctcttca agctgaggca gctgcgatga atccacgaca atttcacaat
    12841 tcgtcagcag gttcgccgca tcacgatcgt caacgccttt cagcttgatg atcatgtcct
    12901 gattgtggtg cttccagctt tccagctgga cttgctgcca ctgacccgcc ttctggataa
    12961 accagggctg atagtcaaaa atgctttcgg cgtcttcggt ggaagaaaac actctgagcc
    13021 acccacgaat accgtaagac gaacccattt ttcccaaaac gatgggatca acaggtgctt
    13081 gcgcggtgag ttgtttgctc atcatgacca ccgtgacaga ttaagctgct ttgtttactt
    13141 ctttgatcag cgcagcaacg cgatcagaaa tagttgcgcc ctggccaacc cagtgagcga
    13201 tgcgatccag atccaggcga gtgccttctt ctttttcgct agcgattggg ttgaagaaac
    13261 caacgcgctc gatgaagcga ccgttgcgtg cattacggct gtcagcgaca acaacctggt
    13321 agaacggacg ctttttagcg ccgtgacgtg ctaaacgaat agttaccata acatcctctt
    13381 gtgtgaataa aacaaccgga ccccatcgag gaacggagtc cggtgtcata ttaaaagccc
    13441 gaaaatttta ctgatttctg gagaaaatgc aatcagcagt taataactct gtcgcataag
    13501 gtcatttagc gaccaggaaa tcccggcggc atcatcccct tcatgcttct catcatcttc
    13561 gccattccgc ccttcttcat tttcttcatc atgcgctgca tgtcgtcgaa ctgcttcaga
    13621 aggcggttaa cgtcctgcac ctgcataccg caaccggcag cgatacgacg cttacgcgaa
    13681 cctttgatga tttctggctt agcgcgctct ttcatcgtca tcgagttgat gatggcttcc
    13741 atacgcacca gcactttatc gtccatctgc gacttaacat tatccgggat ctgccccatg
    13801 cccggcagct tgcccatcag gctagccatg ccgcccatat ttttcatctg gcgcagttgc
    13861 tcaagaaaat cgttgagatc gaagccgtcg ccttttttca gcttgctggc taatttctct
    13921 gcctgcgcgc ggtcaacttt gctttcgata tcttcgatca gcgacagtac gtcgcccatg
    13981 ccgagaatac gcgacgcgat gcggtccgga tggaacggct ccagcgcctc agttttctcg
    14041 ccaacaccga gaaacttgat cggtttgcca gtgatgtgac gaatagagag cgccgcaccg
    14101 ccgcgggcat cgccgtccac tttggtcaac actacgccgg taagcggtaa cgcttcattg
    14161 aatgcttttg ccgtattggc cgcatcctga ccggtcatgg cgtcaaccac aaacagggtt
    14221 tcaaccgggt taatcgacgc atggacttgt ttgatctcgt ccatcatcgc ttcgtcaacg
    14281 tgcagacgac cagcggtatc caccagcagc acgtcgtaga atttcagttt ggcttctttc
    14341 agcgccgcgt taacgatatc taccggcttc tgaccaacat cagaagggaa gaaatcaacg
    14401 cccacctgct ctgccagcgt ctcaagctgt ttgattgccg ccgggcgata aacgtcggca
    14461 gaaaccacca gcactttctt cttgtgcttc tcgcgcagga acttaccgag tttaccaacg
    14521 ctggttgttt taccggcacc ttgcaggccc gccatcagca cgaccgcagg cggttgcgcc
    14581 gccaggttca gggtctggtt ctcttcgccc atcgccgcaa ccagttcgtt acggacgatt
    14641 ttgacgaact cctgccccgg cgtcaggctc ttattaactt catgaccaac cgctttctct
    14701 tttacgcgat tgataaactc acgcactacc ggcagagcga cgtccgcctc cagcagcgcc
    14761 atgcgcactt cgcgcagcgt atcttttacg ttgtcttcag tgaggcgtcc acggccactg
    14821 atattgcgca gcgtgcgcga caaacgatcg gttaaattat caaacattgt ctctcgcctg
    14881 gggtggaaac ggttggccgc aatcgcgaca catcatcagt attttgccgc agtataacat
    14941 gaaggcgtct ttgttgttat gcaacggttg gagcagcgtt cacctgacgc tatactgctt
    15001 ctctttctta ttgctcaaac tgtcgacatt actatgcccg tttttgctct gctcgcgctt
    15061 gtcgcctact ccgtcagtct tgcgctgatt gttcccggtc tgctgcaaaa aaacggcggc
    15121 tggcggcgca tggctattat ttctgcggtc attgcgctgg tctgccacgc aatcgctctg
    15181 gaagcccgca tcctgcccga cggtgatagc ggacaaaacc tcagcctgct gaacgttggt
    15241 tcattggtca gtttgatgat ctgtacggta atgaccattg tggcttctcg caatcgtggc
    15301 tggttgctgc tacccattgt ctatgccttt gcgcttatca acctggcgct ggcgaccttc
    15361 atgcccaatg aatacatcac ccatctggaa gcaacgcctg ggatgctggt gcacattggc
    15421 ttatcgctct tttcctatgc cacgctaatt atcgccgccc tgtacgcgct gcaactggcg
    15481 tggattgatt accaactgaa gaacaagaag ctggcattta accaggaaat gccgccattg
    15541 atgagtatcg agcgtaaaat gttccacatc acgcagattg gcgtggtgct gctaacgctc
    15601 acgctttgca ctggcctgtt ctacatgcac aacctattta gcatggaaaa tatcgacaag
    15661 gctgtgctct ctatcgtggc gtggtttgtc tatattgtgc tgctgtgggg acattaccat
    15721 gaaggatggc gtggacgccg cgtcgtctgg tttaacgttg cgggcgcggt aattctgaca
    15781 ctggcctact tcggcagccg aattgtccag cagttaatca gctaaaccca gaaaaggagt
    15841 ttcccctgga acacatttct actactacgt tgatcattat tctgatcatc atggtggtca
    15901 tttcagccta tttttccggg tccgaaaccg gaatgatgac cctcaaccgc tatcgtctgc
    15961 gacatatggc gaaacagggt aatcgctcgg ccaaacgcgt cgaaaaattg ctgcgtaagc
    16021 cagaccgcct gataagcctg gtgttaatcg gcaataacct ggtcaatatt cttgcctccg
    16081 cgctcggcac tattgttggg atgcgtttgt acggcgatgc gggcgtggca attgcgactg
    16141 gtgtgctgac ttttgtcgta ctggtatttg ctgaggtatt gccgaaaacc attgccgcgc
    16201 tgtacccgga aaaagtcgct tatccgagta gttttctgct ggctccgctg caaattttga
    16261 tgatgccgct ggtctggttg ctgaatgcta tcacccgtat gctgatgcgc atgatgggta
    16321 tcaaaaccga tatcgtggtt agcggctctt tgagcaaaga agagttgcgc actatcgtgc
    16381 acgaatcacg ctcacaaatt tcccgtcgca atcaggatat gctgctgtcg gtgctcgatc
    16441 tggaaaaaat gaccgttgat gacatcatgg tgccgcgcag tgaaattatc ggtattgata
    16501 tcaacgatga ctggaaatcg attctgcgcc aactctccca ctcacctcac gggcgcatcg
    16561 tgctctaccg tgattcgctg gacgacgcca tcagtatgct gcgagtacgt gaagcctggc
    16621 ggttgatgtc ggagaaaaaa gagttcacca aagaaaccat gctgcgcgcc gcggacgaga
    16681 tctattttgt gccggaaggt acgccgctca gcacgcagtt ggtaaagttt cagcgcaaca
    16741 aaaagaaagt cggcctggtc gtcaacgagt atggagacat tcaggggctg gtgacggttg
    16801 aagatattct ggaagagatt gtcggcgatt tcactacgtc gatgtcgcca acacttgccg
    16861 aagaggtcac gccgcaaaac gacggttcgg tgattatcga tggcaccgcc aacgtgcggg
    16921 aaatcaacaa agcctttaac tggcatctac cggaagatga tgcccgcacg gttaatggcg
    16981 tcattcttga ggcactggaa gagatccctg tcgcaggcac ccgcgtgcgt attggcgagt
    17041 acgatataga tattctcgac gtacaggaca atatgattaa gcaggtaaaa gtttttcctg
    17101 tgaaaccgct gcgcgagagt gtggcggagt aacgaaaacg gccctgcatt cgcatgcagg
    17161 gccgtgaatt attacgaaaa cagaaattac gcttttgctt tcgctacagt taccatcgcc
    17221 gcacgaatcg tacgaccatt cagcgtataa cccttctgca taatgcccag tacgttacct
    17281 ggcgcaacgt catcagattc caccattgcg atagcctgat gcacattcgg gtccagcggc
    17341 acgttagttt cggcgatcac ttcaacacca aacttacgca caacatccag catcgacttc
    17401 agcgtcagct caatgccttc aaccatcgcg gacatatccg ggttagcttt atcagccact
    17461 tccagcgcac gatccaggct atcaatcacc ggcagcaatt cgttgatgaa tttctccagc
    17521 gcgaatttgt gggctttttc aatatccagt tcagtacgtc gacgcaggtt ttccatttcg
    17581 gcttttacac gcaaaatgcc gtcacgttca cgggtctggg cttcagccag ctgagcttcg
    17641 agattcgcaa ttttttcatc gcgcgggtcc acctgctcag cagaagcttc tggctcaact
    17701 gcctcaatct cttcgtgctg atccatgata atttcttccg gggcttgccc ctcaggcgtt
    17761 ttctgttctt tactactcat gaatttctcc gcgttttttt cgcattcatc tcgctaactt
    17821 cgcttattat ggggatcagt ttcagggttt caagggaagc actcacattg tcatcaatct
    17881 tcgcaacaag gacctcggaa aaatgaataa tcatttcaag tgtattggca ttgtgggaca
    17941 cccacggcac cccactgcac tgacaacaca tgaaatgctc taccgctggc tgtgcacaaa
    18001 aggttacgag gtcatcgttg agcaacaaat cgctcacgaa ctgcaactga agaatgtgaa
    18061 aactggcacg ctcgcggaga ttgggcaact agctgatctc gcggtagtcg ttggtggcga
    18121 cggtaatatg ctgggcgcgg cacgcacact cgcccgttac gatattaaag ttattggaat
    18181 caaccgtggc aacctgggtt tcctgactga ccttgacccc gataacgccc agcaacagtt
    18241 agccgatgtg ctggaaggcc actacatcag cgagaaacgt tttttgctgg aagcgcaagt
    18301 ctgtcagcaa gattgccaga aacgcatcag caccgcgata aatgaagtgg tgcttcatcc
    18361 aggcaaagtg gcgcatatga ttgagttcga agtgtatatc gacgagatct ttgcgttttc
    18421 tcagcgatct gatggactaa ttatttcgac gccaacaggc tccaccgcct attccctctc
    18481 tgcaggcggt cctattctga ccccctctct ggatgcgatt accctggtgc ccatgttccc
    18541 gcatacgttg tcagcacgac cactggtcat aaacagcagc agcacgatcc gtctgcgttt
    18601 ttcgcatcgc cgtaacgacc tggaaatcag ttgcgacagc cagatagcac tgccgattca
    18661 ggaaggtgaa gatgtcctga ttcgtcgctg tgattaccat ctgaatctga ttcatccgaa
    18721 agattacagt tatttcaaca cattaagcac caagctcggc tggtcaaaaa aattattcta
    18781 attttacgcc agcctcttta ctgtatataa aaccagttta tactgtacac aataacagta
    18841 atggtttttc atacaggaaa acgactatgt tggcacaact gaccatcagc aactttgcta
    18901 tcgttcgtga gcttgagatt gattttcata gcggcatgac cgtaataact ggcgagaccg
    18961 gcgcgggtaa atctattgca atagatgccc tcggtctttg tctcggtggt cgcgctgaag
    19021 ccgacatggt gcgtaccggc gctgctcgcg ctgacctgtg cgcccgtttt tctctgaaag
    19081 atacgccagc ggctctgcgc tggctggaag aaaaccagct tgaagacggg catgaatgtt
    19141 tgcttcgtcg cgtgatcagc agcgatggtc gctcccgtgg tttcatcaac ggtacagctg
    19201 ttcctctgtc acaactgcgc gaactgggtc agttgctgat tcagatccat ggtcagcacg
    19261 ctcatcagtt actcaccaaa cccgagcacc aaaagttcct gcttgatggc tatgccaatg
    19321 aaacctctct actccaggaa atgacagcac gttatcagtt gtggcatcaa agctgccgtg
    19381 acctcgcgca tcatcaacag ttaagtcagg aacgcgctgc ccgtgcggaa ctgctgcaat
    19441 accaattaaa agaacttaac gaatttaatc cgcagcccgg agagtttgaa caaatcgacg
    19501 aagagtacaa acgtctggcg aacagcggtc aattgctgac caccagccag aatgcattgg
    19561 cattaatggc cgacggtgaa gacgcaaacc tgcaaagtca gctttacacg gctaaacaac
    19621 tggtgagcga attgattggc atggacagca aactgtccgg cgtacttgat atgctggaag
    19681 aagctaccat ccagattgct gaagccagcg atgaactgcg ccactactgc gatcgtctgg
    19741 atctcgatcc caaccgacta tttgaacttg aacagcgcat ctcaaaacag atttcgctgg
    19801 cacgtaaaca tcacgtcagc cctgaggcat tgccacagta ttaccagtcg ctactggaag
    19861 aacagcagca actggacgat caggccgact cacaagaaac gcttgcgctg gcggtaacga
    19921 aacatcatca gcaggcactg gaaatcgcgc gcgcattaca ccaacaacgc cagcaatatg
    19981 cagaagaact tgcacagctg atcaccgaca gtatgcatgc gctctcaatg ccgcatgggc
    20041 agtttacgat cgatgttaaa tttgacgagc atcacctggg cgctgacggt gccgatcgta
    20101 ttgagtttcg ggtaaccacc aacccaggtc agccaatgca gcctattgcc aaagtcgcat
    20161 ccggtggtga attgtcccgc atcgcactgg caatccaggt catcacggcg cgtaaaatgg
    20221 aaaccccggc actgattttt gatgaagtgg atgtagggat tagcggtcca acagcggcag
    20281 ttgtcggcaa actgctgcgt caactcggcg aatcaactca ggtgatgtgt gttacccacc
    20341 tgccacaagt cgcgggatgt ggtcatcaac actattttgt cagcaaagaa accgatggtg
    20401 cgatgacaga aacgcatatg caatccctga ataaaaaagc gcggttacaa gagctggcgc
    20461 gcctgcttgg tggcagtgaa gtcacacgta atacactggc gaatgcgaaa gaactgcttg
    20521 cagcgtaaac ttttttcctg cttcacggtc agagtaaaca gcaaaacgcc gtaagaccgg
    20581 aaagcaaaag gttttaaagt gatgaaaggt ctattatcat cggcatatta cagatgagcc
    20641 acgtactgct cgggcccgaa aaggaatcaa atcactatgc gctgtaaaac gctgactgct
    20701 gcagcagcag tactattgat gttgaccgca ggctgttcca ctctggagcg agtggtttac
    20761 cgtcctgaca tcaaccaggg gaactatctg accgctaacg acgtatccaa aatacgtgtt
    20821 ggcatgacgc aacaacaagt tgcgtacgca ttgggtacac cgctgatgtc cgatccattt
    20881 ggtacgaata cctggttcta tgtcttccgc cagcaaccag gtcatgaagg tgtaacgcag
    20941 caaacgctga cgctgacctt taacagtagc ggtgtgttga ccaatattga taacaaacct
    21001 gcgctgagtg gtaactaata ataaagttgc tctcaaagac gttaaaaaag gtgctcaatg
    21061 agcacctttt ttctgtctgt tatttattcg ctgatttttc tgctcgttgc ctgcgaagct
    21121 ctttcggatc ggcaatgaga gggcgataaa tctccacccg atcgccatca tgcacactat
    21181 cgcttagttt tgccggacgg ctgtaaatgc cgactttgtt tttagttaaa tcgatatcgg
    21241 tacgcaattc cagcaagcca ctggcgcgaa tagcttcttc aaccgtcgcg ccctcctgca
    21301 gcgtcactcg ctgcaggtac tgcttctcag gtagcgcata agccacctca acggcaattt
    21361 tacctggcac tgtaaacctc tttcgcacga accgtaaaag cctggaccat attagccgcc
    21421 agctctttaa acacgcgacc aaaggcgagt tcaatcaact tattggtaaa ctcaaagtcg
    21481 agatgaaact cgatacgaca cgcctcctgg ctcagcggcg taaacttcca tccaccaatc
    21541 aatttcttga acggcccatc caccagattc ataagaatac tttggttact ggtcaactgg
    21601 ttgcgggtag taaacgtttt gctgatccca gccttagaga catctaccgc cgcagtcatc
    21661 tgcccaggag tggactccag aatccgactt ccggtacaac ccggcaaaaa ctgaggataa
    21721 gactgaacgt cattcactaa ctgatacatt tgctccgcgc tgtagggtac cagtgcggtc
    21781 cggctaatct gaggcataac aatttccatc aacaaaaatc caacaaataa tatcatttat
    21841 cctgctgtaa aaaaaacgct atcccggcgc tgggtaacat cgggttcatg ctaagataga
    21901 gccttgtccc ccgcaggatt gatatggggt gttttcgatt tcagattacc gatgattcac
    21961 gacgcttatg acgaagaaaa aagcacataa acctggttca gcgaccatcg cgcttaacaa
    22021 gcgcgcccgt cacgaatact ttatcgaaga agagttcgaa gcgggacttg ccctgcaagg
    22081 ctgggaggtt aaatccctgc gcgcaggaaa agccaatatc agcgacagct acgtccttct
    22141 gcgtgacgga gaggcatttc tgtttggcgc taacatcacg ccaatggccg tggcctccac
    22201 gcatgtggtg tgcgatccta cccgtacccg caagttactt ctcaaccaac gcgaactgga
    22261 ctcattgtac ggtcgcgtca atcgagaagg ctataccgta gtggcgctct ccctgtactg
    22321 gaaaaatgcc tggtgcaaag tgaaaatcgg cgtcgccaaa ggtaaaaaac agcacgataa
    22381 acgttcagat atcaaagaac gcgaatggca ggtggataaa gcacgtatca tgaaaaacgc
    22441 ccaccgttaa acctgcactc caattattga ccagttcctc accgcgcctc cctctccggc
    22501 ggcgcgaatg aacatcttat tggctatcac atccgacaca aatgttgcca tcccattgct
    22561 taatcgaata aaaatcaggc tacatgggtg ctaaatcttt aacgataacg ccattgaggc
    22621 tggtcatggc gctcataaat ctggtatact tacctttaca cattggggct gattctggat
    22681 tcgacgggat ttgcgaaacc caaggtgcat gccgaggggc ggttggcctc gta
//
LOCUS       DAMZET010000061        22351 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.061, whole genome shotgun sequence.
ACCESSION   DAMZET010000061 DAMZET010000000
VERSION     DAMZET010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.061"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1405)
                     /gene="creC"
                     /locus_tag="POB34_002419"
     CDS             complement(<1..1405)
                     /gene="creC"
                     /locus_tag="POB34_002419"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HDI5919353.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLR"
     gene            complement(1405..2094)
                     /gene="creB"
                     /locus_tag="POB34_002420"
     CDS             complement(1405..2094)
                     /gene="creB"
                     /locus_tag="POB34_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HDI5919354.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(2107..2580)
                     /gene="creA"
                     /locus_tag="POB34_002421"
     CDS             complement(2107..2580)
                     /gene="creA"
                     /locus_tag="POB34_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HDI5919355.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            2791..3660
                     /gene="robA"
                     /locus_tag="POB34_002422"
     CDS             2791..3660
                     /gene="robA"
                     /locus_tag="POB34_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HDI5919356.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(3657..4304)
                     /gene="gpmB"
                     /locus_tag="POB34_002423"
     CDS             complement(3657..4304)
                     /gene="gpmB"
                     /locus_tag="POB34_002423"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HDI5919357.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            4356..4868
                     /gene="yjjX"
                     /locus_tag="POB34_002424"
     CDS             4356..4868
                     /gene="yjjX"
                     /locus_tag="POB34_002424"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HDI5919358.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            complement(4911..5237)
                     /gene="trpR"
                     /locus_tag="POB34_002425"
     CDS             complement(4911..5237)
                     /gene="trpR"
                     /locus_tag="POB34_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HDI5919359.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(5327..7264)
                     /gene="sltY"
                     /locus_tag="POB34_002426"
     CDS             complement(5327..7264)
                     /gene="sltY"
                     /locus_tag="POB34_002426"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HDI5919360.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            7475..9142
                     /gene="ettA"
                     /locus_tag="POB34_002427"
     CDS             7475..9142
                     /gene="ettA"
                     /locus_tag="POB34_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HDI5919361.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(9449..10681)
                     /gene="nadR"
                     /locus_tag="POB34_002428"
     CDS             complement(9449..10681)
                     /gene="nadR"
                     /locus_tag="POB34_002428"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HDI5919362.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(10702..12084)
                     /gene="radA"
                     /locus_tag="POB34_002429"
     CDS             complement(10702..12084)
                     /gene="radA"
                     /locus_tag="POB34_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDI5919363.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(12133..13101)
                     /gene="serB"
                     /locus_tag="POB34_002430"
     CDS             complement(12133..13101)
                     /gene="serB"
                     /locus_tag="POB34_002430"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HDI5919364.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            13207..13851
                     /locus_tag="POB34_002431"
     CDS             13207..13851
                     /locus_tag="POB34_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HDI5919365.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            13879..14895
                     /gene="lplA"
                     /locus_tag="POB34_002432"
     CDS             13879..14895
                     /gene="lplA"
                     /locus_tag="POB34_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HDI5919366.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            14927..15076
                     /locus_tag="POB34_002433"
     CDS             14927..15076
                     /locus_tag="POB34_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919367.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
     gene            complement(15351..16070)
                     /gene="deoD"
                     /locus_tag="POB34_002434"
     CDS             complement(15351..16070)
                     /gene="deoD"
                     /locus_tag="POB34_002434"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDI5919368.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(16150..17373)
                     /gene="deoB"
                     /locus_tag="POB34_002435"
     CDS             complement(16150..17373)
                     /gene="deoB"
                     /locus_tag="POB34_002435"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDI5919369.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(17425..18747)
                     /gene="deoA"
                     /locus_tag="POB34_002436"
     CDS             complement(17425..18747)
                     /gene="deoA"
                     /locus_tag="POB34_002436"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HDI5919370.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(18874..19653)
                     /gene="deoC"
                     /locus_tag="POB34_002437"
     CDS             complement(18874..19653)
                     /gene="deoC"
                     /locus_tag="POB34_002437"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDI5919371.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            19911..21461
                     /locus_tag="POB34_002438"
     CDS             19911..21461
                     /locus_tag="POB34_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HDI5919372.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NFGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            21433..22296
                     /locus_tag="POB34_002439"
     CDS             21433..22296
                     /locus_tag="POB34_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HDI5919373.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
BASE COUNT         5211 a         6225 c         5743 g         5172 t
ORIGIN      
        1 gtcgtagcga ggccagcacg ccaccttcct gcacgttgcg cagcgtgact tcgccgttaa
       61 acaaacgggc gacctcactg acgaacgcca accccagacc gctgcttttt tgcccatttg
      121 cacgcggcaa agagtaaaag cgttcaaaaa tacgtgacag cgcgtagtca ggaataccac
      181 taccggtatc cagcacctta agggcgacgt gttcctgatc cacttcggcg cttagcgtta
      241 tgcgaccgct ctcgggggta aaatcgatgg cgttatccag taaattcccc agcgcctgct
      301 ccagtaacgc cggttcagca gcaacattaa cctcagtggg cataacatgt agagtgatgt
      361 ttttttctgc caactgcacg gtgcgcgctt cgctgacgcg gcgaaataat gccgccacat
      421 caacaacagt cagaacgact tcctgacgat tctccagtct tgcctggcgt agtaacgttt
      481 ctaccaatgc ctgcatacgc gcattttgcg tcagaatgtt gtcggtaaaa cgagccacca
      541 cttccggtgg cggaccttcg cgtaaaattt ccgccgcgcc acgaatcgcc gccagtgggc
      601 tttttagctc atgggttaac gcataaacat actgctcaat atagtttttc ccttccagct
      661 tcacgcgcat actttccagc gcctgcgcaa gcttacgcaa ctcgctactg ccgagttcag
      721 gaagaggaac gggcttattg tcagtgacgg aatcggcata gcgagtgagc cgggcaatgg
      781 agcggttgat ccaccaaacc atgcctgcgc caatcaccag tgcaatcccc agcaaaatgg
      841 cgctggccca taaaattcgc cgctcgctac gcttaatgac cggagccatc gccgcgttcg
      901 gtttgcctac gctcaaaacg ccaataagcc gcgagccgtc cataatcggc gcggcaacat
      961 acatcaccga actttcggga tcggcaggat tttgcaacgt gctgcgcgca ccatactgac
     1021 cacgcaacgt tagccagacg tcattccagc gcgaataatc ctgcccaacg gctttatttg
     1081 ccgaatcgaa caatacttta ccctgcgaat cggtcatata gacatgatat tcattgcgca
     1141 ctttgttaat gccaccgata ttggcgcgaa acgggcgatg ttgtagctga ttaaacgcct
     1201 gcgccagttg cccatgcgtt gggtccccag agagcaaatc gggacgcgcc agctccgcca
     1261 gcaacgttgc ggtatcgatt aacgtcccct ccgttgctct tcgcacgccc ggtttaactt
     1321 ctttgacaaa aatagccagt acgaaccagg ccgccaccgc caccagtaaa aaatagccca
     1381 gcagcaaccg catgccgata cgcattacag gcccctcagg ctatatccca tgccgcgatg
     1441 agtattaatc ggtgaaagat cggggttgat ggcgcgcagc ttggcacgca acgttttaat
     1501 gtgggtatcg acggtgcgat cgtaggtgtc ctgcgcatct tcccacacgc tatccattag
     1561 ttgctggcgg gaccagacgc ggcctggcga cttgagtaac gtcttcagca ataaaaattc
     1621 ataccgcgtc agcgttaatg gcgtgtcaaa ccagctgatc tgcgccgcgg gttcattcaa
     1681 ttcaaaatga ccaatacgga tgacgggaga cggcgacgag aacttcttca cccgacgcag
     1741 taaggtgcgc accctggcgc acacttcgcg gggtgaaaac ggtttagcca catagtcgtc
     1801 agcgccaatt tccagcccaa gcaggcgatc gacctcttca cttcgggccg tcaggaacag
     1861 tacaggtaac gccggatgga gcgccagtaa ctggcggcac aattcaaagc cgctgatatc
     1921 aggcaaacca acatcgagaa tcatgacgtc gggaacctgc tggcgagctt tatccagcac
     1981 cggcaagcct cgctcaaaga cctcgacggc aaaaccttcc tgctgcaaca tgtagaccag
     2041 cgtgtcggct atcccttgct catcttccac taaccagacc gtttcccgtt gcataaatcg
     2101 cctctgttat tgccgccacg gcatgacagg aaccgcgcta atcgcgtttt tcggcgaacc
     2161 ttctacaact ttgtcggagt aagccagata agcgagcgcg ttgcgtttgg cgtcataaaa
     2221 gcgcacgacc tgtaacgact taaagaccag ggacgtgcgt tttttgaata ctacctcgcc
     2281 ctgagctttg ccgtttttaa tacgatccga cagttcaatc ggcccgactt gctgacaaga
     2341 aatagccgca tcggaggtat cttccgccag acccaatccc cctttaatac cacctgtttt
     2401 cgcccggctc acataacagg tgacattttt cacatcggga tcgtcaaagg cttccacaac
     2461 aattttgtga tccgggccga tcattttaaa tacggtgtcg accgaaccaa tctcttctgc
     2521 atgagccaat ggccccagca ttatcaggct taaagacagg atcaaatgct tgtatttcat
     2581 attgttacca tttttatcta ttacacgagc aattagttcg tcacggtaaa taacatattg
     2641 attaagaaca aaatctcaat acttttattt ccgtcaagcc ctaaaacata ctctactaag
     2701 aaaaaaacac tgaatgctaa aacagcaaaa aatgctatta tccaattacc tgatgtcagg
     2761 tgctcgttgt tgaaaggatg aggatatttt atggatcagg ccggcattat tcgcgacctt
     2821 ttaatctggc tggaaggtca tctggatcag cccctgtcgc tcgacaatgt agcggcgaaa
     2881 gcaggttatt ccaagtggca cttacagaga atgtttaaag atgtcactgg ccatgctatt
     2941 ggcgcgtata ttcgtgctcg tcgtttgtcg aaatcggcgg tcgcactacg cctgactgcg
     3001 cgtccgattc tggacatcgc gctgcaatac cgcttcgact ctcaacagac atttacccgc
     3061 gcattcaaga agcagtttgc ccagactcct gcactttacc gccgttctcc tgaatggagc
     3121 gcctttggta ttcgcccgcc gctacgtctg ggtgaattca caatgccaga gcacaaattt
     3181 gtcaccctgg aagatacgcc gctgattggt gttacccaga gctactcctg ttcgctggag
     3241 caaatctctg atttccgcca tgaaatgcgt tatcagttct ggcacgattt tctcggcaac
     3301 gcgccgacca ttccgccagt gctctacggc ctgaatgaaa cgcgtccgag tcaggataaa
     3361 gacgacgaac aagaggtatt ctataccacc gcgttagccc aggatcaggc agatggctat
     3421 gtactgacgg ggcatccggt gatgctgcag ggcggcgaat atgtgatgtt tacctatgaa
     3481 ggtctgggaa ccggcgtgca ggagtttatc ctgacagtat acggaacgtg catgccaatg
     3541 ctcaacctga cgcgccgtaa aggtcaggat attgagcgat actacccggc agaagacgcc
     3601 aaagcgggag atcgcccaat taatctgcgc tgtgaactgc tgattcctat ccgccgttaa
     3661 cgctgcagct catctaatgc aggggcgtcc agatgcgaga tgtcccctgc ggtttcaacg
     3721 acccagccag acgccagcca caggctttcc tgataatcca cgcgcgaaat agaacagtta
     3781 cgcagacgta agcggcgctc tgcccatgct ggtaatccga gaatcgtact caccaggcat
     3841 cccagtgcaa taccatgact caccagcagc ggtcggcttc cctgcggtaa gtcccggcaa
     3901 gattccagtg ccgcattgac gcgatcgctg agctcctgca ttgactcgcc ttcaggaata
     3961 cgcccgtcaa cggtgccatt gaccagctgc cgacgccagt tctcttcttc ttcggtcaga
     4021 gaatcgatat gtcttttttc cagcacaccc atgtttaatt cacgcaggcg agaatcaaag
     4081 atgatgtcac agccgcaggc ctgggcgatg atttccgccg tacgccgggt gcgtcctaaa
     4141 tcgctactga taatatgcgt aatgccaagc tctttggcac gggttgccac ctgcatcgcc
     4201 tgttgctcac ctttggcggt cagcgggctg tcagactggc cctgaatacg tcgctcggcg
     4261 ttccactgcg tttcaccgtg gcggactagg tatacctgta acatgctttt tttccgtata
     4321 ctgctgtaac gttaattttc tgagtaatgc tgattatgca ccaagttgtc tgtgcgacca
     4381 ccaatcccgc taaaattcag gccattctgc aggcatttca cgagatcttc ggcgaaggat
     4441 cctgccatat tgcatccgtt gccgtcgaga gcggtgtacc ggaacagccc tttggcagtg
     4501 aggaaacgcg cgctggcgca cgaaatcggg tagcaaatgc ccgccgttta cttccagagg
     4561 ctgatttttg ggtggcgatt gaagctggca tcgatggtga cagcactttc agttgggtgg
     4621 tgattgaaaa caccagccag cgcggcgagg cgcgttcggc tactttgccg ttacctgcgg
     4681 tgattctgca gaaagtgcgt gaaggtgagg cgcttggtcc cgtgatgtcg cgttataccg
     4741 gtattgatga gattggtcgt aaggaagggg cgatcggcgt gtttaccgcc gggaaactca
     4801 ctcgcgccag cgtctatcac caggcggtga tcctggcgct gagtccgttt cataatgccg
     4861 tgtattaagc gcctgatgcg acgctgccgc gtcttatcag gcctacaaaa tcaatcgctt
     4921 ttcagcaaca cctcttccag ccactggcgc agctccacgg gcgcggcttt caggctgtta
     4981 gatccacgcg taatcgtcgc gatgcccgcg ccgagttcat tttttaactc acgctgactc
     5041 atttcgccac gcaacagctc ttcgacaata cgcacgcgag tccccaacgc ttcgcgctca
     5101 tctggcgtca gcatcaggtt taacaacggt aaatggagat cgttttggta ggcattctta
     5161 agcaggtcga caaaacgtaa ccactcctgg tgacgctgtt ctgccatcgc tgctgaatag
     5221 ggtgattgtt gggccataat atgtcgccat tgttagcggg ggaagcaaaa tgcctccccc
     5281 ggttgtactc gctaaagagt acgatagcat atcataaacg tgcggatcag taacgacgtc
     5341 cccattccgt ggcgctcatc aacgtcggtt tatcccccat gaaatagcgg tagtaagcgt
     5401 cataagccag cacgttcttc acataaccgc gcgtctcgga gaacggaata ctctcgacaa
     5461 atgccactgc gtcgatacgc ccggcgctgt tgccaagcca ggttcgcacc cgccctggtc
     5521 cggcgttata agctgctgag gagaaaatac ggttattgcc aaactgctga taaacatatt
     5581 gcagataact ggtgccaatg ttgatattcg tttccggatc cagcaattgc ccagggctac
     5641 tataaccggg aatagagaac atcttcaccg tatgggtcgc tgtaccaggc ataatctgca
     5701 tcaggccgct ggcccctacc ggtgatttca cttttggatt ccaggcgctc tcctgacggg
     5761 caatcgccat cgcatagctt tgcgggatct ccttaccgct ggtgtagcgt ttgaaaagat
     5821 cgttgtaagc cagcgggaat cgctcttcca gatgatccca tagcttcccg gcgatcgttg
     5881 cctgaacgct aagatcccac cattggttgt tgaaagcata ccgcgccagt tgagcctgct
     5941 ctgtttttga cttgctcttc accagattgg cccactcgct acgcgcggtg ttatcgagat
     6001 tccagtacat caactcgcgc acgcgcgcca tctccggccc ctgagtcagg gcgctgtcaa
     6061 cattctgcgg cgctttatca atcttcagct catactcttc tccgatgcgt tgtgctgcaa
     6121 ccatcgggta gaaaccacgc tgttgcatga gttgatgcaa aatctctttc gcttcagctt
     6181 cacgtccgcg ttccagcaat aaatccgcct gccagtaacg ccattcatct ttctcttttg
     6241 cttccatcgg cagacgcgcc agccaggtat tcaggccgcg acgatcgccg gtgccaagtg
     6301 ccattcgcac acggcgttca atcaacgacg tggattgcga gcgcataatg gcgtcatcgc
     6361 gccatttcgc ctgctcgtcg gtgacatcgt tgcccatcaa acgccaggcg acgatatcgc
     6421 gcagctcctg aatctgatct tcattaagct gctgcgcctg ggcaagcgac gggatcatca
     6481 gccgtgcatt ttcagcatcc tgccgcgcca cactggcaaa cgccaccgct gccatttgac
     6541 gggtaaaatc ggtcgctccg gttgtacgcg cgaaggtcag taccgtatta gggttgttcg
     6601 ccagtgaaat gattgccgag gcgatagtct ggtaatcggc aggcatctgc cctgccagca
     6661 ctgttaccag gccagtgtta cccgctttca tcgccagacg gatacgctct aaatacgcca
     6721 gcggatcttg tttacctgac gcacgccaga cgctaaataa cttgtcacag gcgttaggct
     6781 ggctcttgcc ggttagccac agctctttcg ccccttgcca ggcttcttca ctctgcccgg
     6841 tgttccattt cgcatagtag taattacatt gcgcttcggt agttccgggc ttttccgggc
     6901 taaaggctaa caagccacgc cagtcttcac gccgcgccag ttcattgacg aaacgagatt
     6961 gcagcgtgcg agcgggagga agcgtggggt tagcgcgaac aaagttagtg accgtcaccg
     7021 ccggttgatt catcagatca tcggtgatct ggcggtattc caggtaggga taaagcggat
     7081 aatccttcag tccaggcatc atttgttcga ccacatccat ttgtcgatta tcccaggcct
     7141 gcttgatttg cgcgtaacgg ctacgctgct catccagtga gtcggctcgc gccacgctgc
     7201 tgaccgtcag cagacagaca ccggcagcca acagccgcca ggtaacttgt ttggcttttt
     7261 ccacaagcgc atcctctaag tgtaaataca tcaatgcagc gtcatgccgc gtaatagatt
     7321 tatgctaacc agtcattgcc gtttacgcca cgttacggac acttttttac ttttactgcg
     7381 aaggtgatcg accaactggg attaggcggg gaaaaaggct acacttcgcc tttgaaaact
     7441 catcatcacg ataaacatag aggcgaagtc caacgtggct caattcgttt ataccatgca
     7501 tcgtgtcggc aaagttgttc cgccgaaacg tcatattttg aaaaacatct ctctgagttt
     7561 cttccctggg gcaaaaattg gtgtcctggg tctgaacggc gcgggtaagt ctaccctgct
     7621 gcgcattatg gcgggcattg ataaagacat cgaaggtgaa gcacgtccgc agccagacat
     7681 caagattggt tatctgccgc aggaaccgca gctgaacccg gaacacaccg tgcgtgagtc
     7741 cattgaagaa gcggtttctg aagtggttaa cgccctgaaa cgcctggatg aagtgtatgc
     7801 gctgtacgcc gatccggatg ccgattttga caagctggcc gctgaacaag gccgtctgga
     7861 agagatcatt caggctcacg acggtcataa cctgaacgta cagctggagc gtgcggcgga
     7921 tgcgctacgt ctgccggact gggacgcgaa aatcgctaac ctctccggtg gtgagcgtcg
     7981 tcgcgtagcg ttgtgccgcc tgctgctgga aaaaccagac atgctgctgc tcgacgaacc
     8041 gaccaaccac ctggatgccg aatccgtggc ctggctggaa cgcttcctgc acgacttcga
     8101 gggcaccgtg gtggcgatta cccacgaccg ttacttcctc gataacgttg caggctggat
     8161 cctcgaactt gaccgcggtg aaggtattcc gtgggaaggc aactactcct cctggctgga
     8221 gcagaaagat cagcgcctgg cgcaggaagc ttcacaagaa gcggcgcgtc gtaagtcgat
     8281 cgagaaagag ctggagtggg tacgtcaggg aactaaaggc cgccagtcga aaggtaaagc
     8341 acgtctggca cgctttgaag agctgaacag caccgaatat cagaaacgta acgaaaccaa
     8401 cgaactgttt attccacctg gaccgcgtct gggcgataaa gtgctggaag tcagcaacct
     8461 gcgtaaatcc tacggtgatc gcctgctgat tgatgacctg agcttctcga tcccgaaagg
     8521 ggcaatcgtc gggatcatcg gtccgaacgg cgcgggtaaa tcgaccctgt tccgtatgat
     8581 ctctggtcag gaacagccgg acagcggcac catcacttta ggtgaaacgg tgaaactggc
     8641 atcggttgat cagttccgtg actcaatgga taacagcaaa accgtttggg aagaagtttc
     8701 cggcgggctg gatattatga agatcggcaa caccgagatg ccaagccgcg cctacgttgg
     8761 ccgctttaac tttaaagggg ttgatcaggg taaacgcgtt ggtgaacttt ccggtggtga
     8821 gcgcggtcgt ctgcatctgg cgaagctgct gcaggttggc ggcaacatgc tgctgctcga
     8881 cgaaccaacc aacgacctgg atatcgaaac cctgcgcgcg ctggaaaacg ccctgctgga
     8941 gttcccgggc tgcgcgatgg ttatctcgca cgaccgttgg ttcctcgacc gtatcgccac
     9001 gcacattctg gattaccagg atgaaggtaa agttgagttc ttcgaaggta actttaccga
     9061 gtacgaagag tacaagaaac gcacgctggg cgcagacgca ctggagccga agcgtatcaa
     9121 gtacaagcgt attgcgaagt aatgcgtaaa atgccggatg cggcgtgaac gccttatccg
     9181 gcctacaaac caagaaattt caatacgttg tgaatctcca ggcctgataa gacgcggtaa
     9241 gcgtcgcatc aggcattgag caccgaatgc cggatacggc gtgaacgcct tttccggcct
     9301 acaacagcct tagtatccgc aaattcgtag cgggtcaccc gtgctgattc ttttaaacct
     9361 tgtttaatct cgacagcctc ttccagagag gttttcaact catcaaaaaa cttattttat
     9421 acctcgcctt tgagccgttt catcgcggtt atctctgctc ccccatcatc tcccgcacca
     9481 gctccacgca gcgcaggaaa cggctgtcat aatcgtcctc ttcaacccgc acgaattcga
     9541 tattgttctc ttccagcatc tccaccagca agttctggaa ctctttgcga tccaccgaac
     9601 tgccgaggct gcgtaaacca tccgccaccc acggcgtgtt gttctccagc aggatcacca
     9661 gatcgaaacg gtattcatca atcaacgcct gtacgaacgg atgctcacgc ccttcgtact
     9721 ttttgcagaa cgcctgggta gtgacaaaat cagtgtcaat aaacgccact ttattggcat
     9781 atttcactgc aaaatcaatg tattgtgcgt ggcccagcgc gattttatcg taatcggaat
     9841 actgcaatgc gatctcatca ccgccgaggt gtgaaaagac ataatcgcga ccatattccc
     9901 acgcactggt ggtattgaag atattggcaa gtttgtttac cagggtggat ttaccgctcg
     9961 actcgccacc aaggatcgcc acggtacgta caaagaacgg cttcacttcc gtaggaatat
    10021 attcccagta gcggaacggg ttttcgcgga tctgcgcacc gctgatactc ataaaggtac
    10081 gtttcggatc gaccagcacc gtatcgatcc ccagatgttc catatactgt ggcgcatcgg
    10141 cttcttccga ggtgtagatc aggtccggct gaatcccctt ttcagccata aactttttga
    10201 tgccgttgct ccacacatcc cagccgtgcg gatacggctc catgccctct tcgttgaaag
    10261 catgaatgcg aatatttttc tgatatttaa aagtttgtaa taaccagcgc agacgatccg
    10321 gcaccgtggg ctgctgcgac atggcgctgt cttcaaacaa cgcgcggtcg cgggtatcgt
    10381 caaaacccat aatgatatgc agctcgtcaa cctggctaca ggcgcgctgg ataaggtaga
    10441 tatgtccggt atgcagcggg tagaacttac cgaatacgac accaatggtt ttcttctgtc
    10501 ggggaaattc aagccccaaa aaacggtgca acgcctccag cttttgcgcg ctggggcttt
    10561 taattttggc attcagtaac tggcttaaat accctttggt cataccgctg gcatcagcta
    10621 cctgctgtag cgtgcagccc tgttgcttga tggcagtttt caggtaatca aatgacgaca
    10681 tatctccctc cgtatctctc attataagtc gtcgaacacg ctaagcgcgt cggagagttt
    10741 tttaacgcca aaaatctgca tcccttccgg cgcttttttc ggtacgttag ccgccggaac
    10801 aatcgcccgg cgaaaaccgt gtttcgccgc ttctgaaatt cgttcctgac cgctgggcac
    10861 cgggcggatc tcccctgcca gcccgacttc accaaacacc accagatcct gtggcagcgg
    10921 tctgtcacgc aggctggaaa ccatcgccag cagtaacgct aaatcggcac tggtttcggt
    10981 taccttcacg ccgccgacca cgttcacaaa tacatcctga tcggccattt gcagaccacc
    11041 gtgacggtgc aagacagcca gcaggattgc cagacggttt tgttccagcc ccactgccac
    11101 gcggcgcgga ttcgccatca tcgagtgatc gaccagcgcc tgaatctcca ccagcaacgg
    11161 acgcgttcct tcccacacca ccatcacgga gctgccggag gtcacttcat ctccgcgact
    11221 taagaaaatt gccgaagggt tgctgacttc acgcagcccc tgttcggtca tcgcgaagac
    11281 gcccagctca ttcaccgcgc cgaagcggtt tttatggctg cgcaaggtgc gaaaacggga
    11341 gtcggcatca ccatccagaa gcaccgaaca gtcgatacag tgttccagca ctttagggcc
    11401 agccagcgag ccatctttgg ttacgtgccc aaccatgaca atcgccacac cgcgcgtttt
    11461 ggcgaagcgc gtcagataag ccgccgtttc acgcacctgc gccacgctac caggcgatga
    11521 ttgtacatcc gccatatgca tcacctggat cgagtcaatt accatcagtt tcggttgctc
    11581 ttcttcggca atcaggcaga tctgttcgat gctggtttcc gacaacatat tgagattgtc
    11641 agtcggcagg ccaaggcgat gagcgcgcat tgccacctgt tgcagcgact cttcgccagt
    11701 gacatacagc gttttcatct gctgggccag tttgcacagc gtttgcagta gcagcgtgga
    11761 tttccccgca cctgggttac cgccaatcag aatggcgctt cctggcacca cgccgccgcc
    11821 tagtacgcgg tcgaactctt taaatccggt ggaaaaacgc ggcagctctt caaggctgat
    11881 atcggagagt ttctggactt ttgccacccc ggcgctaccg gcatagccgc ttagacgctc
    11941 gttacgcgcc accgttggcg acgcagcaag acgcacctcg gtgatggtgt tccaggcatg
    12001 acaggcactg cactgcccct gccagcgcgg ataatcggcc ccgcattcat tacaaacaaa
    12061 ggcgcgtttt ggagcttttg ccacggtgta cctcgttaat gctgtgccgc ccgcaggatg
    12121 acgggcgagc aattacttct gattcaggct gcctgagagg atgcagaata cccccatcag
    12181 gtcagcgtga cggatggtga cttccgtctt ttcattcact tttggcttgg catggtaggc
    12241 aatccccagc cctgctgctt tgatcatcgg caggtcattg gctccatcgc caatcgccac
    12301 ggtctgcgcc agcggaattt catactcttg cgcgaggcgg gtcagagttt tcgctttgta
    12361 ctgcgcgtca acgatgtcgc cgatcacatt gccggtaaat ttaccgtcca tgatctccag
    12421 ttcattggct accacggcgg tcaggcgcag cttgtcgcgc aggtattcag caaagaaagt
    12481 aaagccgccg gaggcaatcg ccactttcca gcccagcgtt tccagcttga gcaccagttg
    12541 cgttaagcct ggcatcagcg gcagattttc acgcacctgt tgcagaatat tggcgtcagc
    12601 gcctttcagc gtcgccacac ggctgcgcag gctggcggta aaatcgagtt cgccgcgcat
    12661 cgcccgttcg gttacttccg ccaccatctc gcccgttccg gccagtttgg caatttcatc
    12721 aatacattca atctggatgg cggtggaatc catgtccatc accagcaaac ccggcgtgcg
    12781 caggtgcggg attttcccca gcggggcgac atccagttgc gcttcatggg ccaggcgcgt
    12841 agcccgtgcg gtgagtgaac ctgccagacg aatcacctga taatcttcca cgcaccaggc
    12901 ggcaacaatc accatcgccg cacccagttt gctctggtat tgggtcagac gctgtttatc
    12961 cagcccacga ccatacagca gccagccgct acgacctgcg tggtaatcca gtggcatcac
    13021 ttcatcacca cttaatgaaa gaggcagacc cggccataaa gagacatctt caggcaggtc
    13081 gcaccaggta atgttaggca ttaaggctcc tgtaaaatcg ttcgaagcag ggaaaataac
    13141 gcatgaggct accttgtatc cattgcttct ggcaacatta agtctcaaat tttcaaaggg
    13201 tggaagatgg ctcgcacaaa actgaaattc cggctgcatc gggcagtgat tgtcctgttc
    13261 tgtcttgcct tgttagtggc actgatgcag ggggcgtcat ggtttagtca aaaccaccag
    13321 cgacagcgta atccacagct ggaagaactg gcccgcaccc tggcgcgtca ggtgacgctg
    13381 aacgttgcac cgctgatgcg taccgactca ccagatgaaa aacgcattca ggcgatcctc
    13441 gatcagttaa cggatgaaag ccgcatcctc gacgcgggtg tgtatgacga acaaggcgat
    13501 cttatcgccc gttcaggcga aagcgtcgaa gtgcgcgacc ggctggcgct cgacggtaaa
    13561 aaagcaggcg gctattttaa ccagcagatt gtcgagccaa ttgcgggtaa aaacggaccg
    13621 ctcggctatc tgcgcctgac gctcgacacc catacgctcg ccaccgaagc ccaacaggtg
    13681 gataacacca ctaacatttt acgcctgatg ttgctgctct cactggcaat cggcgtagtg
    13741 ctgacccgca cgctgctaca gggtaaacgc acccgctggc agcaatcgcc cttcctgtta
    13801 accgccagca aaccggtgcc ggaagaggaa gaaagcgaga aaaaagagtg acccattact
    13861 acaagaaagg aaatcgttat gtccacatta cgcctgctca tctctgactc ttacgacccg
    13921 tggtttaacc tggcggtgga agagtgtatt tttcgccaaa tgcccgccac gcagcgcgtt
    13981 ctgtttctct ggcgcaatgc cgacacggta gtgattggtc gcgcgcagaa cccgtggaaa
    14041 gagtgtaata cccggcggat ggaagaagat aacgtccgcc tggcgcggcg cagtagcggt
    14101 ggcggcgcgg tgttccacga tctcggcaat acctgcttta cctttatggc tggcaagccg
    14161 gagtacgata aaaccatctc tacgtcgatt gtgctcaatg cgctgaacgc gctcggcgtc
    14221 agcgccgaag cgtccggacg taacgatctg gtggtgaaaa cagtcgaagg cgaccgcaaa
    14281 gtctcaggct cggcctatcg cgaaaccaaa gatcgtggct tccaccacgg cactttgctg
    14341 ctcaatgccg accttagccg cctggcaaac tatctcaatc cggataaaaa gaaactggcg
    14401 gcgaaaggca ttacctcagt gcgttcccgc gtgaccaacc tcaccgagct gctgccgggg
    14461 atcacccatg agcaggtttg cgaggccata accaaggcct ttttcgccca ttatggcgag
    14521 cgtgtagaag cggaaatcat ctccccggac aaaacgccag acttgccaaa cttcgccgaa
    14581 acctttgccc gtcagagtag ctgggaatgg aacttcggtc aggctccggc attctcgcat
    14641 ctgctggatg aacgctttag ctggggcggc gtggaactgc atttcgacgt tgaaaaaggc
    14701 catatcaccc gcgcccaggt gtttaccgac agcctcaacc ccgcgccgct ggaagccctc
    14761 gccgggcgac tgcaaggctg cctgtaccgc gcggatatgc tgcaacaaga gtgcgaagcg
    14821 ctgttggttg acttcccgga ccaggaaaaa gagctacggg agttgtcgac gtggatagcg
    14881 ggggcggtaa ggtaactcgc tgattaaaat accaaaggaa gggaatatga tcgatctgga
    14941 aaaccaggag cgggagatta tcaatttaat gttaagccag cgtatttcct ggctagccgc
    15001 cgtgcgtatc aggcataaat tatcgcttgc tgaagtaagt aaaatgttag gtatctcgat
    15061 taattctcta aaatagatcg aaaagacgga acggtttagc tcaaacatca aaagcaaaat
    15121 ggcagaaatt tatggttgtc caccagagtt gctgatatgc ccttcctgga tgacggctga
    15181 acataaatga atagattcgt gaatagatac ttgaatagat tttattttct atccatatga
    15241 aaaataatag taaatctata aatcagttgc tattgatgaa tagatactaa aaagccggag
    15301 cagtctccca ctccggcttc acacgacaat cgcctttcag cgaaacacaa ttactcttta
    15361 tcgcccagca gaacggattc cagtgcgatt ttgatcatgt cgttgaaggt ggtctgacgc
    15421 tcagcggcag tggtctgctc gtgagtgcgg atgtggtcag acacggtaca gatggtcagg
    15481 gctttcgcgc caaattctgc agcgacgccg tagataccag ccgcttccat ttccacgccg
    15541 aggatgccgt atttttccat cacgtcgaac atttcgccgt ccggagagta gaacaggtca
    15601 gcggagaaca ggttaccaac gcgagcatca atgcccagtg ctttagccgc gtctaccgcg
    15661 ttacgcacca tgtcaaagtc agcgatagcg gcaaagtcat gatctttaaa acggatgcgg
    15721 ttaactttgg aatcggtgca ggcacccata ccgataacga cgtcgcgcag ttttacgtgc
    15781 ggcagaactg cgccacagga acccacgcgg ataattttct tcacgccgaa atcggtgatc
    15841 agttctttgg tgtagatgga gcaggacggg atacccatac cgtgacccat tacggaaatt
    15901 ttgcggcctt tgtaagtacc ggtgaagccc agcataccgc gaacgttgtt cacttcacgg
    15961 gcatcttcaa ggaaagtttc agcaatatac ttcgcacgca gcgggtcgcc tggcatcaaa
    16021 actacgtcag cgaaatcgcc catttctgca ttaatgtgtg gggtagccat tgttttatcc
    16081 ttatcaacat gttttcaccc tctccctgag ggagagggcc ggggtgaggg catcaagccg
    16141 cacccattat cagaacatgg ctttgccata ttccatatca gaagtaccaa aatattttgc
    16201 cagagtctgg ccgatatccg cgaaggtttc acggtgaccc agtgagcccg gttttacttt
    16261 cgggccgtaa accagtaccg gaatgtgttc acgcgtgtgg tcagtaccgg tccaggtcgg
    16321 atcacaaccg tggtcagcag tgaggatcag gatgtcgtca tcgcgcagca gagacatcag
    16381 ctccggcaga cggcggtcga acagctccag acccgcagca taaccggcga cgtcgcgacg
    16441 gtggccccag gaagagtcga agtcaacgaa gttggtgaag acgatggtgt tatcacccgc
    16501 ttctttcatc tctttgatgg tggcgtcaaa cagcgcgtcc aggccagttg ctttcacttt
    16561 cttggtgata ccgcagttgg cgtagatgtc tgcaatttta ccgacagaaa ccacctgacc
    16621 gtgtttttca tcaaccagtt tctgcagcac ggtcggtgct ggcggctcaa cagccaggtc
    16681 gtgacggtta ccggtacgct ggaagttacc ggctttgtca ccgataaacg gacgagcgat
    16741 aacacgaccg atgttgtagc cgccgttggt cagctcttca cgggcgattt cgcacagttc
    16801 gtagagttta tccaggccaa aggtttcttc gtggcaggca atctggaaca cagagtcagc
    16861 ggaggtatag aaaatcggct tgccggtttt catgtgctct tcgcccagct gatccagaat
    16921 gaccgtaccg gaagagtggc agttaccgag gtaacccggc agattagcgc gttcaaccag
    16981 tttatccagc agctcttgcg ggaagctgtt ttcgtgatcg gagaaatatc cccactcaaa
    17041 tagaaccggg acaccggcga tttcccagtg accagacggg gtatctttac cggatgacat
    17101 ttcgtgcgcc catgcgtacg cgccgataac ttcagcgttg ccgtccattc ccgccggaat
    17161 gaaaccggta gaaccttcgt gtgctttcgc cagccccaga cgggtcagat ttggcagatt
    17221 gagcggacct ttacgaccgt tatcagcttc gcctttggca caagcttctg cgatatgacc
    17281 cagggtgtca gccccgacgt caccaaagcg ttctgcatct tctgtagcgc cgatgccgaa
    17341 tgagtccagc accataataa atgcacgttt catatgttct ccgtactcag tgcttcaaat
    17401 ttaaatgatc agatcagtat accgttattc gctgatacgg cgatagacag ttggtgtgct
    17461 ttccggtgct ttatcggcaa gtttaattgc cgctttcacc gctttcgccg cttcctgcca
    17521 gctgttttcg tctttcgcgt ggataaccgc cagcggacgc tgaccgtcta cctggtcgcc
    17581 caaacgcgcc atatcagtaa agccgacgct gtaatcaatg gtgtcagatg cctgacgacg
    17641 accgccgccc attgcaacca ctgccatccc cagcgcgcgg gtatccattt cactgacaaa
    17701 accttcggta tcagcataga ctgctttcgt cagcatcgct gtcggcagat acttcgcgta
    17761 gttctcaacg aagtcggttg ggcctttttg tgccgctacc atacgaccaa agacttctgc
    17821 cgctttaccg ttgtccagca ctgcctgcaa tttcgcgcgc gcttcggcgt catctttcgc
    17881 cagtttgccg gagataagca tctccacgca cagcgccatc gtgacatcaa acagacgcgg
    17941 gttacgatac tcacccgtca ggaactgcac cgcttcacga acttcaaccg cgttacctgc
    18001 actggaggcc agtacctgat tcatgtcggt gagcagcgcg gtggtgcgca cgccagcgcc
    18061 gttagccacg ccaacaatcg cttcggcaag ggcttcagag agttcgtagg tcggcataaa
    18121 cgcgccgcta cccactttca cgtccatcac cagcgcgtcc agaccttccg ccagtttctt
    18181 cgccagaata gaggcggtga tcagcgggat ggagtccacg gttgcggtaa tatcacgggt
    18241 cgcgtagaaa cgtttatccg ccggagccag tgagctggtc tgaccgataa tcgccacgcc
    18301 gacgtcttta ataatttcgc ggaaacggtt gtcatccggg aaaatgtcga agccagggat
    18361 ggattccagt ttgtcgagcg taccgccagt atgaccgagg ccgcgaccag agatcatcgg
    18421 aatatagccg ccgcaggctg cgaccatcgg ccccaacatc agcgaagtca catcgccgac
    18481 gccaccagtg gaatgtttat caacaatcgg gccattcaga tgcaggcttt tccagtcgag
    18541 aacggttcct gaatctcgca tcgccatggt cagcgagaca cgctcaggca ttgtcatatc
    18601 gtggaagaaa atggtcatcg cgagggcggc aatctgccct tcggagatag tgttgtcgcg
    18661 aatgccgttg ataaagaaac gaatttcttc atcgctcagt gcatgaccat cacgtttttt
    18721 acgaataatt tcttgtgcga gaaacaaggt aacctcccga ggaaaagagt atgaggcggc
    18781 ttttgccgga tgcgtctacg ccttatccgg cctacgggta gatacgtctc gtaggcctga
    18841 taagcgcagc gcatcaggcg taaagcatct tacttagtag ctgctggcgc tcttaccgtc
    18901 accgtggccc agcgctttca acaggcttgc cagcaggctg gaagcaccaa agcggtagtg
    18961 acgcgcatct gcccagtcag caccgaacag ttcatctgcg atggcgagat atttctgcgc
    19021 atcttccgca gtacgcacgc cgcccgctgg tttgaaacca acggtttttt ctacgcccat
    19081 atcacggatc acttccatca tgatgcgcgc gctttccggc gtcgcgttca cagccacttt
    19141 accggtagag gttttgatga agtccgcacc cgctttgatg gagatttcag acgctttacg
    19201 gatcagcgct tcgtctttca gttcgccggt ttcgatgatc actttcagca gtacattcgc
    19261 tgccgcgcaa gcctctttac aggctttcac caggtcaaaa ccaacctgct cgttacccgc
    19321 catcagcgcg cggtacggga acaccacgtc aacttcatcg gcaccgtagg cgattgccgc
    19381 acgggtttct gccagcgcga tttcgatgtc atcgttaccg tgtgggaagt tggttaccgt
    19441 agcaatacgg atttccgggg tgccctgctc tttcagtgtt ttgcgagcaa tcgggataaa
    19501 gcgaggatag atacagatag cggcggtatt gccgaccggg gttttggcct gatgacacag
    19561 agcaattact ttctcgtcgg tgtcgtcgtc attcagggtg gtcaggtcca tcaatttcag
    19621 tgcacgcagg ctgcttgctt tcagatcagt catttcattc tcctggcttg tcgccaataa
    19681 aattcacctt gcgagtttgt tagtattcta acatctactc cgcaacacac ttcgatatac
    19741 atcacaatta aggaaatcta cttacaagtt tgcatcactg taatgcgatc tggttcaaat
    19801 aattcacttt caaatgaatg cgtcagtggt ggcaaacgca tcaggatcaa agtgaacatc
    19861 acgaaacttc ttacaatggc gcatcacctt cggcataaaa ggaaaagaag atgcccactt
    19921 ctcatgaaaa tgcactgcaa caacgttgcc agcaaattgt caccagccca gtgcttagcc
    19981 cggagcagaa gcgccatttt ctggcactgg aagcagaaaa caatctgcct tacccacagc
    20041 tgcctgccga agcccgccgc gcgctggatg aaggtgtaat ctgcgatatg tttgaaggtc
    20101 atgcgccgta caaaccgcgc tatgtcttac ccgattacgc ccgttttctg gcgaacggtt
    20161 ccgaatggct ggagctggaa ggcgcgaaag atcttgatga cgcactctct ctgctgacca
    20221 ttctttacca ccacgtaccg tcggtcacat cgatgccggt ctacctgggg caactggatg
    20281 cgttgttgca accgtatgtt agaattctaa cacaagacga gatcgatgtt cgaataaaac
    20341 gtttctggcg ttacctcgac agaaccctgc cagacgcctt tatgcacgcc aatttcggcc
    20401 cgtctgattc gcccattacc cgtgcaatct tacgtgcaga cgcagagttg aagcaggttt
    20461 caccgaacct gacctttatc tacgatcctg aaatcacccc tgatgacctg ctgctggaag
    20521 tggcgaagaa catctgtgaa tgtagcaaac cgcacatcgc caacggtccg gtgcatgata
    20581 aaattttcac aaaagggggc tacgggattg tgagctgtta caactcactg ccgctggcgg
    20641 gtggtggcag cacgctggta cgccttaacc tgaaagccat tgccgagcgc agcgaatcgc
    20701 tggatgactt ctttacgcgc actctaccgc actactgcca gcagcagatc gccatcatcg
    20761 atgcgcggtg tgaattcctc tatcaacaat cacacttctt tgagaatagc ttcctggtga
    20821 aagaagggct gattaaccct gaacgttttg tgccaatgtt tggcatgtat gggctggcgg
    20881 aagcggttaa cttgctgtgt gagaaagaag ggattgccgc acgttacggt aaagaagccg
    20941 ccgcaaatga agtgggttat cgcatcagcg cgcaactggc ggagtttgtc gccaataccc
    21001 ccgtgaaata tggatggcaa aaacgcgcca tgttacacgc acagtcgggg atcagttccg
    21061 atattggcac cacgccgggc gcgcgtttgc cgtatggcga tgagccagat ccgatcaccc
    21121 atctgcaaac tgtcgccccg catcatgctt attattattc cggcatcagc gacattctga
    21181 cgctcgacga aaccatcaaa cgtaacccgc aggcactggt acagctttgc ctcggtgcct
    21241 ttaaagccgg aatgcgtgaa tttaccgcca atgtcagcgg taacgatctg gttcgcgtta
    21301 ccggttatat ggtacgtttg tcggacttag aaaaatatcg cgccgaaggt tcacgcacca
    21361 acaccacctg gctgggcgaa gaagccgcac gcaacactcg tattctggaa cgccagccgc
    21421 gcgtgataag ccatgaacag cagatgcgct ttagtcagta agattatccc cttctcctgc
    21481 gttgacgggc caggcagtcg tctggctctg tttttgcagg gctgcaatct gcgctgcaaa
    21541 aactgtcaca atccgtggac gatgggacgc tgcaatgatt gcggagagtg cgtgccacag
    21601 tgtccgcatc aggcgttgca gattgttgac ggcaaagtgc tgtggaacgc tgtggtctgc
    21661 gagcagtgtg atacctgcct gaagatgtgt ccgcaacatg ccacgccaat ggcgcaatcc
    21721 atgagcgtgg acgaagtgct tagccatgtc cgcaaagcgg tgctgtttat tgaagggata
    21781 acggtaagcg gcggcgaagc cacgacccaa ctgccgtttg tagtggcgct gtttactgct
    21841 atcaaaaacg atccgcaact gcgccacctc acctgcctgg tggacagtaa cggcatgttg
    21901 agcgaaaccg gctgggaaaa attactcccg gtgtgtgacg gcgcaatgct cgatctcaaa
    21961 gcgtggggga gcgaatgtca tcaacactta accggacgcg ataatcagca gattaagcgc
    22021 agcatctgtt tgcttgcaga gcgcggcaag ctggcggaac tgcgtttgct ggtaattcca
    22081 gaccaggtgg attatttgca tcacatcgat gagctggcga cgtttatcaa gagacttggc
    22141 gatgttccgg ttcgcctgaa tgcgtttcat gcccacggcg tgtatggcga ggcgcaaagc
    22201 tgggcgagcg ccacgccgga agacgttgag ccgttggctg atgcgttaaa ggtgcgcggg
    22261 gtgagccggt tgatatttcc ggcgctctat ttgtgacatg ccggatacgg cctacggttc
    22321 ggcacagact tgtaggcatg ataagacgcg a
//
LOCUS       DAMZET010000062        22203 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.062, whole genome shotgun sequence.
ACCESSION   DAMZET010000062 DAMZET010000000
VERSION     DAMZET010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.062"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1438)
                     /gene="cydA"
                     /locus_tag="POB34_002440"
     CDS             complement(<1..1438)
                     /gene="cydA"
                     /locus_tag="POB34_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDI5919374.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGVSAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLIC"
     gene            complement(2033..2260)
                     /locus_tag="POB34_002441"
     CDS             complement(2033..2260)
                     /locus_tag="POB34_002441"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919375.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKIITLGELIII"
     gene            complement(2285..4918)
                     /gene="mngB"
                     /locus_tag="POB34_002442"
     CDS             complement(2285..4918)
                     /gene="mngB"
                     /locus_tag="POB34_002442"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDI5919376.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEQIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVMTSATAKPQCEITHEAWQSRAVIR
                     YDMAVPLNLSERSVRQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(4936..6852)
                     /gene="mngA"
                     /locus_tag="POB34_002443"
     CDS             complement(4936..6852)
                     /gene="mngA"
                     /locus_tag="POB34_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HDI5919377.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            7021..7743
                     /gene="mngR"
                     /locus_tag="POB34_002444"
     CDS             7021..7743
                     /gene="mngR"
                     /locus_tag="POB34_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HDI5919378.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(7847..8716)
                     /gene="sucD"
                     /locus_tag="POB34_002445"
     CDS             complement(7847..8716)
                     /gene="sucD"
                     /locus_tag="POB34_002445"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDI5919379.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(8716..9882)
                     /gene="sucC"
                     /locus_tag="POB34_002446"
     CDS             complement(8716..9882)
                     /gene="sucC"
                     /locus_tag="POB34_002446"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDI5919380.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(9976..11193)
                     /gene="odhB"
                     /locus_tag="POB34_002447"
     CDS             complement(9976..11193)
                     /gene="odhB"
                     /locus_tag="POB34_002447"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDI5919381.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(11208..14009)
                     /gene="sucA"
                     /locus_tag="POB34_002448"
     CDS             complement(11208..14009)
                     /gene="sucA"
                     /locus_tag="POB34_002448"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDI5919382.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(14310..15026)
                     /gene="sdhB"
                     /locus_tag="POB34_002449"
     CDS             complement(14310..15026)
                     /gene="sdhB"
                     /locus_tag="POB34_002449"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HDI5919383.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(15042..16808)
                     /gene="sdhA"
                     /locus_tag="POB34_002450"
     CDS             complement(15042..16808)
                     /gene="sdhA"
                     /locus_tag="POB34_002450"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HDI5919384.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(16808..17155)
                     /gene="sdhD"
                     /locus_tag="POB34_002451"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(16808..17155)
                     /gene="sdhD"
                     /locus_tag="POB34_002451"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HDI5919385.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(17149..17553)
                     /gene="sdhC"
                     /locus_tag="POB34_002452"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(17149..17553)
                     /gene="sdhC"
                     /locus_tag="POB34_002452"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDI5919386.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            17934..18041
                     /locus_tag="POB34_002453"
     CDS             17934..18041
                     /locus_tag="POB34_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919387.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            18247..19530
                     /gene="gltA"
                     /locus_tag="POB34_002454"
     CDS             18247..19530
                     /gene="gltA"
                     /locus_tag="POB34_002454"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HDI5919388.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            19921..20487
                     /locus_tag="POB34_002455"
     CDS             19921..20487
                     /locus_tag="POB34_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDI5919389.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            20538..>22203
                     /locus_tag="POB34_002456"
     CDS             20538..>22203
                     /locus_tag="POB34_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDI5919390.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTTGINAGAWRLRSDYQLNQTDSDDNHEQSGGIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDERMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     SMPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSI"
BASE COUNT         5518 a         5916 c         5365 g         5404 t
ORIGIN      
        1 cgcaaatcag caccattgag aagatgagat cgcctgcggt cagtgacgag ttcgccacag
       61 ctgtcggcag cacttcaccg atagcccacg gttggcggcc atattcagcc acgaaccagc
      121 ccgcttctac agcaatccac ggcagcggaa taccgtacag cgcggcgcgc agaagccatt
      181 ttttctcgcc aatgcggttg cggatgacac tccagaaaga gagcgcgatg attgccagaa
      241 gcaggaagcc acacgccacc atgatacgga acgcaaagta cagcggcgct acacgcggga
      301 tggagtcttt ggttgcctgt tgaatctgcg cttcagtcgc atcagccacg tttggcgtat
      361 agcgtttcag cagcagaccg taaccgaggt ctttcttcat gctattgaac tggtcacgaa
      421 ccgcctggtc ggtagaacca gaacgcagtt gttcgagcag agagtacgcc ttcatcccgt
      481 tacgaatgcg ttcttcatgc tgcaccatca gctctttcag gccgataacc ggggtatcca
      541 cggaacgcgt tgcaatgatg cccagtgcgt aagggatctg aatcgcaaat ttgttcgtct
      601 cctcttcctg atcaggaatg ccgaacagag taaaggcagc aggcgcaggt tgcgtttccc
      661 actcggcttc aatagcagcc agtttggttt tctgcacgtc gcccatttcg tagccggatt
      721 catcacccaa aacaataaca gacagaacag cagccatacc gaagctggca gcgatagcaa
      781 aggagcgttt agcgaaggcg aagtcacgac ctttcagcat ataccatgcg ctgacaccga
      841 ggatgaacat cgcgccagta acataaccag acgctacagt gtgaacgaat ttcacctgag
      901 caaccgggtt aagcaccagc tcggagaagc tcaccatctc catacgcata gtttcaaagt
      961 tgaaatcgga cgcgattggg ttttgcatcc agccgttcgc aaccagaatc cacagtgcgg
     1021 acaggtttga accgagcgcc accagccagg tgacacacat atgctgaact ttacccagac
     1081 gatcccaacc gaagaagaac agacctacaa aggtggattc gaggaagaag gccatcagac
     1141 cttcgattgc cagcggcgca ccgaagatat cccctacata gtgggaatag taagaccagt
     1201 tagtcccgaa ctggaactcc atggtcagac cggtagccac ccccagagcg aagttgatac
     1261 caaacaactt gccccagaac ttggtcatat ctttataaat ctgtttgccg gagaggacgt
     1321 agaccgtttc cataatggcc agcaggaacg ccataccgag cgtcagtggc acaaaaagga
     1381 agtggtacat cgcggtcaag gcaaactgta agcgcgacag ttcgactata tctaacatca
     1441 tgactccttg ctcatcgcat gaagactccg agagtgaacc ccgttagaaa gggtcacacg
     1501 catgccccaa tagaaatttg tttgaacact ttcatcatac catgtgaatg cacacaaaaa
     1561 cggcgtaaag aaaggttgca aacatgttaa taaaaactca aattgatccc acgtatatat
     1621 tacgccgcaa aatccttaca ataaacaggt ttttgttgaa gcaaatttgc atttttcgac
     1681 ggtgatcaat ttatagctaa attaccgcct ttcagccaat ttgatcgaga acaatttatc
     1741 tctttttgat gcccatttcc aagacttata cattgataaa tatcaattcc tacaaattat
     1801 gttaatcatg tttcaataag gtaactttag tgacatttat gtttaaaatg tgtgagttat
     1861 aaggctgaaa ttagtttcgg ttggcaatta aagataaaaa gagacgaagc aaaaacagca
     1921 cggttgacct taattctttt tttggtcact ggctaatacg acgcagagtt tcaggattac
     1981 taataaaggc ataaatccac cccgccgata ttaacattta attaacttat aattatatta
     2041 taattaactc acccagggtt attattttgt taaaaataaa gccacatata cgtggcttga
     2101 gagtgaagat aaacgcaaaa ttacctgatg cgctacgctt gccagtgtct acatcatctc
     2161 tgcaatatat tgaatttgcc tgcttttgta gaccgaataa agcgttcatg ccgtatccgg
     2221 catgaacaaa gcgccctttg tcaacaatct ggccgcgcat atgtggcttc ggtttgattg
     2281 ctattcaggc aagccggtaa ctgaacgtcc gtgactggcc gggtaaaaaa ggccccgata
     2341 gatttgaacc ttgattttcc tcggtggtaa tgtgttcatc catcatcgtt tctgagcaag
     2401 aaatcacctc gcgactgaaa gcaacagtcg catcacaggt tgctgattca gccggattaa
     2461 acagccgtaa aatcacttct tgtcggtctt cagctttctt aagtgcgctt atcaggcatc
     2521 ccactggggg cattttcaac aaactataac tttccggcac gttgaatccg gctttgttga
     2581 gcttcatcgc atcccatggg attttgttgt aacactgtac tggagtcagc catgctcgcg
     2641 cctgctgagc tacaccagcg gcggttggcg taccggtata actcaataaa cttaggcgac
     2701 aagaaagcag accacgtagt tgtgagtccg ggactggcat tttaattccc gaaggccgcc
     2761 caggccttaa aagcagatct tctttgccca gtaagcccac gccacgcagc aacgtaatgg
     2821 caaaggtttt cttctcttca ccgatgactt caaattcacg tagcccttcg ctaaagacag
     2881 ccatgccgtt acgcccttcc tgtaaggcaa catagttgag catattccat accggaaccg
     2941 gcgcttcttt ccagccttct tgctgccagt tgttcattgc actgtcgttc acggggcgcg
     3001 ttagcgaacc aaactgcgta tctgccagaa cactgtcggt gttaaaaggt gtagggatca
     3061 ggacgcgaag gcgatgatcg tcagcctggt tatcaaggtt gatatccaca tcaatacgtc
     3121 tgctgttatg actcagagtg acaaccatct ccacccctac tctgcccgtg gattgccgga
     3181 cgctgcgttc tgacaaattg agcggcaccg ccatgtcata gcggataaca gccctgctct
     3241 gccaggcctc atgagtaatc tcgcattgcg gtttcgctgt tgcagaagtc attacccact
     3301 cttcttttgc gggtgaatag tcatattcat caccatcatc agagctttct tcaatttgca
     3361 acacccggtc atagcgcaca ccgctatctt tatctaccag ttgtagcgtt ccatcctcat
     3421 tgagcgcaat ttgccagaaa gcattttcca gtatcccttc agcgtcactt tttgccgcaa
     3481 tcacgttgcc aggctgattc gcttcgatat aaagcgtgcg atagcccata gaagggacaa
     3541 tctggttgat ctgtatatca aactccataa agggatcgta attaccgtaa tgaactattt
     3601 gccgatcgat taggcctgga tcgatctcac gggcatggcg aataaaatac ggtacaggct
     3661 gaccgcgatc gtctcgtaga ttaaactggc tggcgcgcag ccgcacagtg gtgttaataa
     3721 cttcttcccg cggccagggc atcaggttaa acagcacgag tttgtcggca tcactctgtg
     3781 gcatgttatc ggcaattttg cgcatgtaga aacgcagcag attatccgcc atgtcttcag
     3841 ccagttcgaa gcgggcgaca atttcgcgat gaactttgtc actgcaacaa caaccgatac
     3901 tgtcgtgggc atgatttttt aagatctctt tccacatttt ctccagcaag ccgtggtggt
     3961 attcaaaacc caacgtccag gccagcgttg ccagcggttc cagcagatta acaatcttat
     4021 tttcaatacg tgcgtgggca actttgatat ccatgcgcgt ggaaccgatg gtgcgatgca
     4081 cgcgcatata tttgccatca ataaattcac ctttcagggt cgccagactt tcacgctgcg
     4141 cttcaatctg ctcgaatacc tcttcaaagc ggctcatcac aaactttcgt tgaggataaa
     4201 tttcgcgaag tttctccatc acttcgaaga tattttgctg caatggcatc tggtcatgac
     4261 cattcggcaa taaaatctct ttggttacag acgctttttc cagcacgtca aaataactgt
     4321 cgaggcgttt gcgtaatccg ttttcgtcgg caggtaagta cttaccgatg gcataaccca
     4381 acggcagcac ctgcgccgtc acttcgctac cgtcactgct ttgccataaa aactcggttt
     4441 tatcagtacc gtggcgctcc gaacatccgc gccagaacat agtgcgggta atgccaaatc
     4501 cattgtagat atgcggcagt tgcccggaca tgccaaagga atccggtaaa taacctattt
     4561 tcatcggctc gccaaacgcg aggcagtcac gcattccgta catcagatta cggacgatgg
     4621 attccgcaga aacaatcgtg gtatcggtct gggtatacca ggggccgata atcaacttac
     4681 cggcttctac ctgtttcttc acgcggtctt tgttttccgg tttcaccgcg aaataatctt
     4741 cgaggatcgc cgtttgcccg tcgagtacgt aatatttgta ttcgttgtcc tgttccagtc
     4801 ggcacaggat ctcttccata ttattgacca gcagaatgcg tgactcttcg gtggtgaaat
     4861 accactcccg atcccaatgc atatgcgggg tgatgtgaac gcgagatacc gctttcatcg
     4921 tcgtttcctt tttgtttatg gcattacgcc atcagtcaga tagttgccat gcttaaccgc
     4981 gtgacgccgc cagatcagga gaattgcagt cgagattgca gcccccacca atgccgcacc
     5041 aaaccagcca attgccgcca taacaccgcc cgcgccatta tcatgaagta aaaagagcga
     5101 gaaaatgccg gcaccgggtg tcgaaaggcc gatattcatc gcaccgacaa tagcgcccgt
     5161 taccatagag cccagcacaa acgaaccaat aacccgcagc ggatcttcaa tcgccatcgg
     5221 gatcgcccct tcggtaatac ctgccagccc taacagccag gtggatttcc cggtctcaat
     5281 ttcaaactct ttaaacaggc gcggtgcgag catcgtggaa gcggttacgg taaatgccga
     5341 aaccattttg acggaggcga aaatggcata cgggccgtaa acgccgttcg ccattgcgcc
     5401 caggcagaat gcataagcgg ctttgttcac tggccctcca aggtcaaagg aacacataaa
     5461 accgagaatg gcacccagca acagcgcgtt acttcctgac agaccgttca gccaggcggt
     5521 aagcgagtta ttgatccagg cgacaggttc tcccaccaca aacagcatca gactgcccgc
     5581 tcccaacgta ccgagcaccg ggtagagata aaaagtcagg aatccattga atttactgct
     5641 aagacgcaag tgatttttca cccagcgcat caagtaaccg gctatcaatc cgccaacgac
     5701 cgcgccgaga aacccggagc cgatcatgtt ggcggcaagt ccagccgcaa agcctggcgc
     5761 taacgccggt ttatctgcca gagaataggc ggtataggcc gcgagcaccg gtaccatcaa
     5821 aattccgagc agcccgccgc ccagcttgcg gtacatccac agccaggagt tttcttcatt
     5881 aaacagatct tgtagcccga agatttgcga cagtaatacc gcgaccgcca gcaccgtgcc
     5941 ccccgcgaca atcaacggta cggcaaaaga gattccgctc aacagtgcct gtttcagctc
     6001 cgttttgaca cttttcaccg gttgcgtatc ttgctgtacg gtacgcgtct catcgctacg
     6061 cttgagggta agcgcttgtt ggatcaacgc ttctgcatgg cgaatcggct cggcaacagg
     6121 cactgaaagc gcgggaatgc cattaaaacg ctcactctcc ttgatggcga cttcagccgc
     6181 aaaaatacag gcggttgcac tattgagttg atccgccgtt aaacgccctt caatgccgtt
     6241 agcgccttgt ttttcaacgt aaacatttac gccgagtttg cgtccggctt tttccagata
     6301 ttccgcagcc atataggtgt gagcaatacc cgccggacag gccgttacgc agacgatagt
     6361 tggcgcgttg gaaaaagagg cagaaggttg cgtgcctccc ttgtcatcca gcgccgagag
     6421 caactcatca ggcgtcgtcg ccgactgtat acgcgcccga atctcatcat ccgccaggcg
     6481 cgtggtcagc gctgtcagca gttgcatatg cgtagtaccc gcttcattgg ggggaatcgc
     6541 cagcagcacc actaaatcaa ccgcttccgg gccatcaacg ccttcccact gaagcggctc
     6601 gctgagtgtg gcgaccgcaa acgccgcttc ttttaccgca gcagttttgc catgcggcac
     6661 agccaaccct tcacctaagg cggtcgggcc aaggctttca cggcgataca cttcttccag
     6721 aaattgctca gtactggaaa ttttccccag agcagcaaga cgttgagtca acgcgtggat
     6781 ggcctcttca cggctggtaa agcgcgcatt cagacacaac gcatcgcggt gggtcagagt
     6841 cgtcagattc atcatgatcc tcacttgatc gtttttatag tcgcgccagt gtgcccgata
     6901 aaacaatacc attatgtgat caatttcact tgattgaaat tatttatatt gtatttgtat
     6961 tatttacagg atgaatttat cgggcataat ggctaaattc gtattaatga gatactaaaa
     7021 atgggacaca agcccttata ccggcagatt gccgatcgca ttcgtgaaca aattgcgcgt
     7081 ggcgagctta aacctggcga tgcgttacct accgaatcgg cattacaaac agagtttggc
     7141 gtcagccggg ttacggtgcg tcaggctttg cgccagttag tcgagcaaca gatcctcgaa
     7201 agcattcagg gcagcgggac ttacgtcaaa gaagagcggg tcaattacga tatttttcag
     7261 ttaaccagtt ttgatgaaaa actgtcggac cgtcacgtcg atacacacag tgaagtcctg
     7321 atatttgaag tgattccggc tgacgatttt cttcagcaac agctacaaat caccgcgcag
     7381 gatcgtgtgt ggcatgtgaa gcgcgtgcgc tatcgcaagc aaaagccaat ggcgctggaa
     7441 gaaacctgga tgccgcttgc gttgttcccg gatctcacct ggcaggtcat ggaaaattca
     7501 aaatatcact ttattgaaga agtgaagaaa atggttatcg atcgtagcga acaggaaatc
     7561 attcctctga tgccaaccga agagatgagc cgtctgctta atatcagcca gacaaagccg
     7621 attcttgaaa aagtatcgcg tggatattta gttgatggtc gggtgtttga atatagccgc
     7681 aacgctttta ataccgatga ctataaattt accctgatag cgcaacgaaa atcatcgcga
     7741 taagcacaaa aaaggccatc atacgatggc caaccatgtc gaaaccgaca gcccttcgcc
     7801 attcctgtag agggaagcgg cgagggctat ttcttattac agatatttat ttcagaacag
     7861 ttttcagtgc ttcaccgata tccgccaggc tgcgaacggt tttcacgcct gcggcttcca
     7921 gagcagcgaa tttctcatcc gcagtccctt tcccaccggc aatgatggca cccgcgtggc
     7981 ccatacgttt gcctttcggc gcagtcacac cagcgatgta acccacaact ggcttggtaa
     8041 catgctcttt gatgtacgct gccgcttctt cttcagcgct accgccgatc tcaccgatca
     8101 ttacgatcgc ttcggtctgc ggatcttttt cgaacatttc gagaatgtcg ataaagttag
     8161 agccagggat cgggtcaccg ccgataccaa cacaggtcga ctgaccgaaa ccgtaatccg
     8221 tggtctgttt aaccgcttca taggtcagtg taccggaacg ggaaacgata cccactttac
     8281 ccggtttgtg aatgtgaccc ggctggatac cgattttgca ttcacccgga gtgataacgc
     8341 ccgggcagtt cgggccgatc atacgaacgc cggcttcgtc cagtttcact ttcacagtca
     8401 gcatatccag cgtcgggatg ccttcagtga tggtgataat cagtttgatg cctgcatcaa
     8461 tagcttccag aatggagtct ttgcagaacg gtgctggtac gtagataaca gaagcggtag
     8521 cgccagtagc agcaacagct tcacgcacgg tgttgaacac cggcaggccg aggtgggtag
     8581 taccgccttt acctggggtt acgccgccaa ccattttagt gccgtatgca atggcctgtt
     8641 ctgagtggaa agtcccctgg ctaccagtaa agccctggca gataaccttg gtgtttttat
     8701 caattaaaat ggacattatt tcccctccac tgcggcaaca acctgctgag ctgcatccgt
     8761 cagacctttt gctgcaataa tattcaggcc gctgtcagcc agtttcttcg cgccgagttc
     8821 ggcgttgtta ccttccagac gtaccacgac cggtacgtta acacccactt ctgctaccgc
     8881 gccgatgata ccgtcagcga tcaggtcgca acgaacgata ccgccgaaga tgttaaccag
     8941 aacggctttc actttgtcgt cagagaggat gattttgaac gcttcggtta cacgttcttt
     9001 ggttgcgccg ccgccaacgt caaggaagtt agccggttcg ccgccgtgca gtttaacgat
     9061 gtccatcgta cccatcgcca gacctgcgcc gttaaccata caaccgatgt taccgtccag
     9121 cgcaacgtag ttcagttccc actgtgcagc ctgtgcttca cgcggatctt cctgcgactg
     9181 gtcacgcatt tcgcgcagat caggctggcg gaacagtgcg ttgccgtcag cgcccagttt
     9241 gccgtcgagg caaatcagat cgccctgttt ggtgatgacc agcgggttga tttcgatcaa
     9301 cgccaggtcg cgctccagga aaatggtcgc caggcccatg aagattttgg tgaactgctg
     9361 aaccagttta ccttccagac ccagtttgaa cgccagctcg cgtccctgat acggcatcgg
     9421 gccagtcagc ggatcaagcg caactttgtg gatcaggtgc ggagtttctt ccgccacttt
     9481 ttcgatttcc acgccgcctt cggtggaggc cataaagacc acacgacggg aactacggtc
     9541 tacaacggca ccgagataca gctctttagc gatatcggtc gctgcttcaa ccagaatctg
     9601 gttaaccggt tggccattgg catctgtttg atacgttacc agacgcttgc cgagccagtt
     9661 ttctgcaaaa gcacggatgt cttctttgct gtttacaact ttcacaccgc ccgctttacc
     9721 gcggccacca gcgtgaacct gacatttcac tacccacggg ccggcaccga tttttgaagc
     9781 ggcttcttct gcttcgcgcg gagtagtaca ggcataaccc accggtgctg gtaagccata
     9841 gcgggcaaaa agttgttttg cctgatattc atgtaagttc atgtgttctg tccatccttc
     9901 agtaatcgtt atcttttaaa ccgtagaccg acaccctatc cacgtcggtc tacagtgcag
     9961 gtgaagctta aactactaca cgtccagcag cagacgcgtc ggatcttcca gcaactcttt
    10021 gatcgttacc aggaagccca cggattcgcg accatcgatc agacggtgat cgtaggacag
    10081 cgccaggtac atcatcggca ggatctcaac ctgaccattc accgccatcg gacgatcttt
    10141 gatagcgtgc atacccagaa ttgcgctctg cggcgggttg atgatcggcg tagacatcag
    10201 ggaaccgaac acaccaccgt tggtgatggt gaagttacca ccggtcagat cttcaacggt
    10261 cagcttgccg tcacggcctt tgactgccag ctctttgatt ttcttctcga tgtctgccat
    10321 gccgagggta tcgacatcac gcagtaccgg tgtcaccagg ccgcgcggcg tagaaaccgc
    10381 catgctgacg tcgaaatagt tgtggtaaac cacgtcatcg ccgtcgatag acgcgttcac
    10441 ttccgggtaa cgtttcaggg cttcaaccac cgctttcacg tagaaggaca taaagcccag
    10501 acggatgccg tggcgttttt caaacgcttc accgtactgc ttacgcagat ccataatcgg
    10561 cttcatgttg acttcgttga acgtggtcag catggcggtg gagtttttcg cttccagcag
    10621 acgctctgcc acacgcttac gcaggcgagt catcgggaca cgtttttcac tacgtgcagc
    10681 cagagccggt tgcgccgccg gagcagccgc tgccggagca gactctttcg ccggggcttt
    10741 cgccagatgt ttttccacat cttcacgagt cagacgacca cccacaccgg tgcctttaat
    10801 ggcgctggcg tcgagattgt gttcagccag caggcgacgg atcgccgggc ttaacgcatc
    10861 gttgttttgc tcttccagag atgcctgctg gcgttgcgcc ggagtggacg ctttctcttc
    10921 agatttggcg ctggtttctt taccggcgct gttgccttca cgcaggcgac caaggatctg
    10981 acgagacgtt accgttgtac cttcatcttc cagaaccgca tccagaatgc cgtctgctga
    11041 tgccggtact tccagtacca ctttgtcagt ttcgatttct accagcactt catcacgtac
    11101 gactgcgtcg ccgggttttt tatgccaggt tgcgacggtg gcatcggcta cggattcagg
    11161 caggtcaggg accagaatat ctacgctact cattgtgtat cctttattta ttcgacgttc
    11221 agcgcgtcat taaccagatc ttgttgctgt ttctggtgaa cggacatata ccctaccgcc
    11281 ggagaggcgg aggccgggcg gcctgcataa cgcagagaag ccccaaacgg aatcacttca
    11341 cggaaatgat gctggctgca gtaccatgcg ccctggttga gcggctcttc ctggcaccag
    11401 acaaaatcct tgacgtgagc aaactgttgc aacacttcct gcatcgcttt atgcgggaac
    11461 gggtagagtt gctcgatacg cacaatggcg acatcgtgtt gattgttctt acgacgctgt
    11521 tccagcaggt cgtaataaac cttaccagaa cacatcacta cgcgcttcac gcccttcgga
    11581 tcaagctcgt cgatttcacc gatggctggc aggaaggtgc cgttcgccag ttcttcgagg
    11641 ctggaaaccg ccagcggatg acgcagcagg gatttcggcg acatcacgac cagcggacga
    11701 cgcatcccgc gcagcgcctg acgacgcagc atgtggtaaa cctgtgccgg ggtagacggt
    11761 acgcacacct gcatgttttg ctcagcacaa agttgcagat aacgttccag acgcgcggag
    11821 gagtgctccg gcccctgccc ttcgtaaccg tgcggcagca acatcaccag accacacatc
    11881 cggccccatt tctgctcgcc agaggagatg aactggtcga taaccacctg cgcaccgttg
    11941 gcgaagtcac cgaactgcgc ttcccagatg gtcagagtgc gtggttctgc ggtggcataa
    12001 ccgtattcaa acgccagcac tgcttcttca gacagtacgg agtcccagac gcggaacgcg
    12061 ccctgcccgt tatggatgtg ttgcaacggc gtgtaagtgg aaccgttaga ctggttgtgg
    12121 atcaccgcgt ggcggtggaa gaaggtaccg cgaccggagt cttcacccga caggcgaacc
    12181 ggaatgcctt cgtcaaccag cgtggcgtaa gcgaggtttt ccgcaccgcc ccagtcgaac
    12241 agtttctcac cggcagccat cgcctggcga tcgccataaa tcttggcaac gcgagactgc
    12301 atttcaactg cttccggcac cgtgctgatg cgtttcgcca gctcctgcag gcgcttcatc
    12361 tcaactttgt tcggatactc ttcgtcccat tcgtggttga gatacggcga ccaggtgaaa
    12421 gagtgcatgt tcatcggacg ccactctgcc actacgcaat cgccagcatc cagcgcatcg
    12481 cggtacaggt taaccatctc ggtggcatct tccagcgtcg ccactttttc ctgctccagc
    12541 ttgtcagcat agattttgcg cggcgtcgga tgttttttga ttttctgata catcagcggc
    12601 tgggttgcgc tcggctcgtc ggcttcgttg tggccgtgac ggcggtagca caccaggtcg
    12661 atgaagacat cacgtttaaa ggtgttacgg aaatcgagcg ccagacgggt cacaaaggca
    12721 acggcttccg gatcatccgc gttaacgtgg aaaatcggtg cctgaaccat cttaccgata
    12781 tcagtacagt acggtgtaga acgagcatcc agcggattag aggtggtgaa accaacctgg
    12841 ttgttgataa cgatacgtac cgtaccgcca acttcataac cacgcgcttt cgacatgttc
    12901 agagtttcct gaaccacacc ctgcccggtc actgcggcgt caccgtggat ggtgattggc
    12961 agcactttgt tgctgctcgg ctcatcaagt ctgtccagac gggcacgaac agaaccgata
    13021 actaccgggc ttacaatctc aaggtgagac gggttaaacg ccagcgccag gtgcaccagg
    13081 ccgccatcgg tctggaagtc agacgagaag cccatgtggt atttcacgtc acccgtgccg
    13141 aggtgttctt tatgtttacc ggcgaactcg tcgaacaagt cttgcggttt tttacccagc
    13201 acgttcacca gcacgttcag acgaccacgg tgcgccatcc cgagaaccac ttcgcgggtg
    13261 ccgctgttgc cagcgtgtcg gatcatctct ttaagcatcg ggattaacgc gtcaccgcct
    13321 tccagcgaga agcgttttgc gccagggaat tttgcgccga ggtaacgttc aaggccttca
    13381 gcggcggtca gttcgcttaa gaagcgtttt ttctcttcgc tattgaaagt cgcgcgacca
    13441 gactcgatac gctgttggat ccagcgtttt tcttcggtgc tggtaatgtg catatactcg
    13501 gcaccaatcg ggccacagta ggtttgcttg agggcttcca gcagctcgcc gagtttcatg
    13561 gtttctttgc cgctggcaaa tgaaccgacg ttgaaggtct cctggaagtc tgcttcggtc
    13621 agatcgtgga aagacggatc cagatcggcc actttatctt gctgccacag tcccagcgga
    13681 tcgagattcg catgctggtg accacggaag cggtatgcgt taatgagctg caggacttta
    13741 acctgcttca cattggtgtc agggtcggag atcgttgaag agtaacgtga agcgtctttc
    13801 gccaggcggc ggaaatattc acgcgtttga gagtggaatt gatccggttt gactcccgta
    13861 ccaggtaact gctggaacgt cgaacgccag ttagcgtcaa ccgagtcagg atcggttaag
    13921 aagtcttcat agagctgttc tatccagctc tggtttgcgc cagagaggta agaagagtcc
    13981 aaccaggctt tcaaagcgct gttctgcatc gtgatccctt aagcatcttt tttatgctta
    14041 cttcgccgtg gatactacca cgcacagtga tgtgcgtgcc ggggttcact tgcgagctct
    14101 tgtctatgta gtggctcgcg aaggaacctt taaaaactgt cttagtgtca ggggcagttt
    14161 ttaaagattt cctgcatttc tttgttacgc ctgatgcgct gcgcttatca ggcctacggg
    14221 taatgacttg taggccggat aaggcgttca cgccgcatcc ggcactggtt gcctgatgcg
    14281 acgcttgcgc gtcttatcag gcctacggtt tacgcattac gttgcaacaa catcgactta
    14341 atatggccga tggcgcgcgt cgggttcagc cccttcggac atacactgac gcagttcatg
    14401 atgctgtgac agcggaatac gctgaatgca tcgctcaaac cgtcgaggcg gctgtcagtc
    14461 tcggtatcac ggctatcgat caggaaacga tatgccgcta acaagcctgc cgggccgata
    14521 aacttatcgg gattccacca gaaagacgga caagaggttg aacaacatgc gcagagaata
    14581 cattcataca gcccgtcgag tttttcgcgc tgctctggca tctgtaaatg ctcgcgagct
    14641 ggcggatttt gtccattatt caacaggtaa ggcttaattt tctcatattg cgcatagaat
    14701 tgtcccatgt ctaccaccaa atcgcggatc accggtaaac ctggcagcgg gcgaatcaca
    14761 atcttcttgc ccggctggtt gagtgccgaa atcggggtaa tacaggccag accattcttg
    14821 ccgttcatgt tcagaccgtc ggaaccgcac acaccttcac ggcaggagcg gcggaacgac
    14881 aggctgggat ctttctcttt cagctggata agcgcatcca gcagcatcat gtcgcgacct
    14941 tcttccgctt ccagggtgta atcctgcata cgcggagcat catcaacatc cgggttatag
    15001 cgataaattg aaaactcgag tctcattttc ctgtctccgc attagtaagt acgaatcttc
    15061 ggcgggaatg ccgggcgcag tttcggttcc atgttgacgc ttcggcgcgt catggattcc
    15121 gactctggca gatacaggga gtggcacagc cagttttcat catcacgatc cgggaagtcg
    15181 aagcggctat gtgcgccacg gctttcggta cggaagttgg cagaaacagc cgttgcatac
    15241 gccgtttcca tcaggttatc cagttccagg cactcaacgc gctgggtgtt gaactcgctg
    15301 gaagtgtcat ccagacgggc atttttcaga cgctcgcgga tcactttcaa ctgctcaagc
    15361 cctttcgcca tcgcatcacc ttcacggaag accgagaagt tatgctgcat acattcttgc
    15421 agcgctttac gaatcgccac cggatcttca ccgttacgat tattgttcca gcggttcaga
    15481 cgatccagag atgcttcaac atcagactcg ctggcatcgc gcaatgcgcc ctgttcggcg
    15541 atagactctt gcagatgcag acccgctgca cgaccaaaga ccaccaggtc cagcagcgag
    15601 ttgccgccca gacggttagc gccgtgtacc gatacacaag cgatttcacc aacggcaaac
    15661 agtcccggaa caaccacatc ttcgcctttc tcgttcaccg tcagcgcctg accggtcact
    15721 ttggtcggaa taccgcccat catgtagtga caggttggga taaccggaat cggctctttc
    15781 accggatcaa cgtgagcgaa ggtacgggag agttccagga tacccggcag gcgggattcg
    15841 agaacttctt tacccaggtg gtcgagtttc agtttcgcgt gtggccccca cggaccatca
    15901 cagccgcgac cttcacggat ttcgatcatg atggaacgcg ccaccacatc acggcccgcc
    15961 aggtctttgg cgttcggcgc ataacgctcc ataaagcgtt cgccatgttt gttcagcaga
    16021 taaccgcctt caccacggca accttcggtg accagtacgc ccgcaccggc aataccggtc
    16081 gggtggaact gccacatttc catatcctgc accggtacgc ctgcgcggat agccatgccg
    16141 acgccgtcgc cagtgttaat gtgggcgtta gtggtggact gataaatacg ccctgccccg
    16201 ccagtcgcca gcacggtagc gcgggctttg aaataaacca cttcaccagt ttcgatgcac
    16261 agtgcagtac aacccaccac cgcgccatcc tggtttttca ccagatccag cgcataccac
    16321 tcggagaaaa tagtggtgtg gtttttcagg ttctgctgat aaagggtatg caacagtgcg
    16381 tgaccggtac ggtcagctgc tgccgcagtg cgtgccgcct gctcgccgcc gaagtttttc
    16441 gactgaccgc caaacggacg ttgatagata cgaccatcat caagacgcga gaacggcagg
    16501 cccatgtgtt cgagttccag aatcgcttcc ggcccggttt tacacatata ttcaatcgcg
    16561 tcctggtcac cgatatagtc cgaacctttt acggtgtcgt acatatgcca ttcccagtta
    16621 tcttcatggg tattacccag cgcaacggta atgccgcctt gcgcagaaac ggtatgggaa
    16681 cgggtcggga agactttaga gagcagcgca caggtttgac cgctctggga aatttgcagc
    16741 gccgcgcgca tacctgcgcc accggcacca atcacaactg catcaaattc tctgactggc
    16801 aatttcatca cacaccccac accacaacga atccataaat cacgtaaacc accagagcaa
    16861 cgacaatcac cagttgcagc atcaggcgca aggccagcgg tttaacgtag tcggtcaaca
    16921 cctgccacat gccgatccag gcatggatca agatagaaaa cagcgccagc agggtgaaca
    16981 ctttggtgaa cgcagaggcg aagaaaccga tccagacttc atatgtcagc tcgccactgg
    17041 tagcgaaaaa accgaccata taaatgatgt agagcgtcag gacgatagcg gtagcgcgaa
    17101 cgaggatgaa atcatgtacg ccattgcgtc ctaatgcgga ggcgttgctt accatacgag
    17161 gactcctgcg agaagtgaaa gcacgacagt aataacaaag gagattttgg cggagcgttt
    17221 acccgcttcg aatgtttctt ccagatagcc aaaatccatc atcatgtggc gaatacctac
    17281 gacgacgtga tacgccagag cggtaaggat gccccacatg ataaatttga cgaagaagct
    17341 gcccataatc gcggaagctt gctcgaaacc ttcaggggaa gagaggctgg tacccagaag
    17401 ccacagcagg atgcccactg caacaaaggt gatcacaccg gaaacgcgat ggagaatgga
    17461 cgctatcgcc gtgatgggga accggatggt ctgtaggtcc agattaacag gtctttgttt
    17521 tttcacattt cttatcatga ataacgccca catgctgttc ttattattcc ctggggacta
    17581 cgggcacaga ggttaacttt ctgttacctg gagacgtcgg gatttccttc ctccggtctg
    17641 cttgcgggtc agacagcgtc ctttctataa ctgcgcgtca tgcaaaacac tgcttccaga
    17701 tgcgaaaacg acacgttaca acgctgggtg gctcgggatt gcagggtgtt ccggagacct
    17761 ggcggcagta taggctgttc acaaaatcat tacaattaac ctacatatag tttgtcgggt
    17821 tttatcctga acagtgatcc aggtcacgat aacaacattt atttaatttt taatcatcta
    17881 atttgacaat cattcaacaa agttgttaca aacattacca ggaaaagcat ataatgcgta
    17941 aaagttatga agtcggtatt tcacctaaga ttaacttatg taacagtgtg gaagtattga
    18001 ccaattcatt cgggacagtt attagtggta gacaagttta ataattcgga ttgctaagta
    18061 cttgattcgc catttattcg tcatcaatgg atcctttacc tgcaagcgcc cagagctctg
    18121 tacccaggtt ttcccctctt tcacagagcg gcgagccaaa taacaaacgg gtaaagccag
    18181 gttgatgtgc gaaggcaaat ttaagttccg gcagtcttac gtaataaggc gctaaggaga
    18241 ccttaaatgg ctgatacaaa agcaaaactc accctcaacg gggacacagc tgttgaactg
    18301 gatgtgctga aaggcacgct gggtcaagat gttattgata tccgtactct cggttcaaaa
    18361 ggtgtgttca cctttgaccc aggcttcact tcaaccgcat cctgcgaatc taaaattact
    18421 tttattgatg gtgatgaagg tattttgctg caccgcggtt tcccgatcga tcagctggcg
    18481 accgattcta actacctgga agtttgttac atcctgctga atggtgaaaa accgactcag
    18541 gaacagtatg acgaatttaa aactacggtg acccgtcata ccatgatcca cgagcagatt
    18601 acccgtctgt tccacgcttt ccgtcgcgac tcacatccaa tggcagtcat gtgtggtatt
    18661 accggcgcgc tggcggcgtt ctatcacgac tcgctggatg taaacaatcc tcgtcatcgt
    18721 gaaattgccg cgttccgcct gctgtcgaaa atgccgacca tggccgcgat gtgttacaag
    18781 tattccattg gtcagccatt tgtttatccg cgcaacgatc tctcctatgc cggtaacttc
    18841 ctgaatatga tgttctccac gccgtgcgaa ccgtatgaag ttaatccgat tctggaacgt
    18901 gctatggacc gtattctgat cctgcacgct gaccatgaac agaacgcctc tacctccacc
    18961 gtgcgtaccg ctggctcttc gggtgcgaac ccgtttgcct gtatcgcagc aggtattgct
    19021 tcactgtggg gacctgcgca cggtggtgct aacgaagcgg cgctgaaaat gctggaagaa
    19081 attagctccg ttaaacacat tccggaattt gttcgtcgtg cgaaagataa aaatgattct
    19141 ttccgcctga tgggcttcgg tcaccgcgtg tacaaaaatt acgacccgcg cgccaccgta
    19201 atgcgtgaaa cctgccatga agttctgaaa gagctgggca ccaaagatga cctgctggaa
    19261 gtggctatgg agctggaaaa catcgcgctg aacgacccgt actttatcga gaagaaactg
    19321 tacccgaacg tcgatttcta ctctggtatc atcctgaaag cgatgggtat tccgtcttcc
    19381 atgttcaccg tcattttcgc aatggcacgt accgttggct ggatcgccca ctggagcgaa
    19441 atgcacagtg acggtatgaa gattgcccgt ccgcgtcagc tgtatacagg atatgaaaaa
    19501 cgcgacttta aaagcgatat caagcgttaa tggttgattg ctaagttgta aatattttaa
    19561 cccgccgttc aaatggcggg ttaattttta tatgcctaaa cacaaaaaat tgtaaaaata
    19621 aaatccacta tcggacattc atatattcta aaaaaaaata tatcggtata aattataaac
    19681 aaatcgatga tttttaatca tctatcttcc tctcagttac atgaataaac caccaaatat
    19741 agcgcacccc tataatttcc aatactttat tcatcaaaca tttaattcat accatcaatt
    19801 aattactttt acatttaata aaattaataa tatttttaag aaaaactaaa aacattcgac
    19861 aatcaaataa gatattcttg caccgatata cttttttctt ttctgttaaa ggactccgtt
    19921 atgtttaaag gacaaaaaac attggccgca ctggccgtat ctctgctgtt cacagcacct
    19981 gtttatgcgg ctgatgaagg atccggtgaa attcacttta aaggtgaagt tattgaagca
    20041 ccgtgtgaaa tacatcagga tgatattgat aaagaggttg aactcggtca ggtgaccacc
    20101 agccacatta atcagtcaca tcacagcgat gccgttgctg tcgacctgcg cttagtcaac
    20161 tgtgatctgg aaaactccag caacggttcc ggtggcaaga tttctaaagt tgcagtcacc
    20221 tttgatagct cagcgaaaac caccggcgca gatccgattc tcaacaacac cagcacgggt
    20281 gaagccaccg gcgtcggcgt acgtttaatg aataaagatc aaagcaatat cgttttaggc
    20341 accgctactc cagatatcga cctggctccg acctccagcg aacaaacgct gaatttcttt
    20401 gcctggatgg aacaaattga tcaggctaca cctgtaacgc caggcgcagt tacggcgaat
    20461 gcaacctacg tgctggatta taaataagta ttactttggc ggcgctatcg ccgcctttca
    20521 taaaatattt atcaggtatg gacaccgtga atatttatcg actctctttt gtatcctgcc
    20581 tggtcgtggc gatgccttgc gcattggcgg tcgaattcaa ccttaatgtt ctcgataaat
    20641 caatgcgcga ccgcattgat atttcattat taaaggaaaa aggagtcatt gctcccggtg
    20701 agtattttgt tagcgttgcg gtaaataata accaaatcag taacgggcaa aagattaact
    20761 ggcacaaaaa tgacgataaa accattccgt gcatcaatga tttactggtc gataaatttg
    20821 gcttaaaacc tgaagtccgt cagtcgttac cattgataaa tcagtgcgtc gattttagct
    20881 cccgacctga aatgctcttc aatttcgatc aagccaatca gcaactaaat atcaccattc
    20941 cgcaagcctg gctggcgtgg cactcagaaa actggacccc accctccaca tggaaagaag
    21001 gtgtcgccgg tgtcctgatg gattacaact tgtttgccag cagctaccgc ccacaggatg
    21061 gcagcagcag cactaacctg aacgcctacg gtaccaccgg aattaacgcc ggggcatggc
    21121 gcttacgtag tgattatcag ttgaatcaga ctgatagcga tgataaccat gaacagtcag
    21181 gcggaatatc gcgcacctat ctttttcgtc cattaccgca attaggctct aaattaaccc
    21241 tcggcgaaac ggattttagt tccaatattt tcgacggttt ttcttatacc ggcgcggcac
    21301 tggcaagtga cgagcgaatg ttgccatggg aactacgcgg ctacgcccca caaattagcg
    21361 gtattgcaca gaccaatgcc acggtgacga tcagtcaatc aggccgcgtc atttaccaga
    21421 aaaaagtccc accaggccca tttatcattg acgaccttaa tcagtctgtt cagggcacac
    21481 tggatgtcaa agtgacggaa gaagatggtc gggtgaacaa tttccaggtt tcggcagcat
    21541 cgatgccctt cctgactcgt cagggacagg ttcgctataa actggccgcg ggtcagccac
    21601 ggccctccat gtcacatcaa actgaaaatg aaaccttttt tagcaatgaa gtttcctggg
    21661 ggatgctgtc aaacacctcg ctgtacggcg gcctgctgct ttctggtgat gactaccatt
    21721 ctgccgcaat gggtattggg caaaatatgc tgtggcttgg tgcgctgtcg tttgatgtca
    21781 cgtgggccag tagccatttt gatactcagc aggacgagcg gggcttaagc taccgtttta
    21841 attacagcaa acaagtggat gccaccaaca gcacgatttc cctcgccgct tatcgtttct
    21901 ccgatcgtca ttttcacagc tacgccaact atcttgatca caaatacaac gacagcgatg
    21961 cgcaggacga aaaacagacg atcagcttat ctgtgggcca accgattacc ccactaaacc
    22021 tcaatcttta cgccaacctg ctacatcaaa cctggtggaa tgcagacgcc tccacgaccg
    22081 ccaacatcac ggcaggtttt aatgttgata ttggtgactg gagagatatc tctatttcga
    22141 cgtcattcaa tacaacccat tacgaagata aagatcgcga caaccagatt tacctgtcga
    22201 ttt
//
LOCUS       DAMZET010000063        22181 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.063, whole genome shotgun sequence.
ACCESSION   DAMZET010000063 DAMZET010000000
VERSION     DAMZET010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.063"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(224..1006)
                     /locus_tag="POB34_002457"
     CDS             complement(224..1006)
                     /locus_tag="POB34_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDI5919391.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(1003..2076)
                     /locus_tag="POB34_002458"
     CDS             complement(1003..2076)
                     /locus_tag="POB34_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HDI5919392.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(2198..2359)
                     /locus_tag="POB34_002459"
     CDS             complement(2198..2359)
                     /locus_tag="POB34_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HDI5919393.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(2486..3091)
                     /gene="osmY"
                     /locus_tag="POB34_002460"
     CDS             complement(2486..3091)
                     /gene="osmY"
                     /locus_tag="POB34_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HDI5919394.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(3484..5073)
                     /gene="prfC"
                     /locus_tag="POB34_002461"
     CDS             complement(3484..5073)
                     /gene="prfC"
                     /locus_tag="POB34_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDI5919395.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVQASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(5164..5841)
                     /gene="yjjG"
                     /locus_tag="POB34_002462"
     CDS             complement(5164..5841)
                     /gene="yjjG"
                     /locus_tag="POB34_002462"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HDI5919396.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(5856..6302)
                     /gene="rimI"
                     /locus_tag="POB34_002463"
     CDS             complement(5856..6302)
                     /gene="rimI"
                     /locus_tag="POB34_002463"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDI5919397.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(6271..6684)
                     /gene="holD"
                     /locus_tag="POB34_002464"
     CDS             complement(6271..6684)
                     /gene="holD"
                     /locus_tag="POB34_002464"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HDI5919398.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            6787..7818
                     /gene="rsmC"
                     /locus_tag="POB34_002465"
     CDS             6787..7818
                     /gene="rsmC"
                     /locus_tag="POB34_002465"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HDI5919399.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVCHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            8086..8172
                     /locus_tag="POB34_002466"
     tRNA            8086..8172
                     /locus_tag="POB34_002466"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8120..8122,aa:Leu,seq:cag)
     gene            8201..8287
                     /locus_tag="POB34_002467"
     tRNA            8201..8287
                     /locus_tag="POB34_002467"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8235..8237,aa:Leu,seq:cag)
     gene            8322..8408
                     /locus_tag="POB34_002468"
     tRNA            8322..8408
                     /locus_tag="POB34_002468"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8356..8358,aa:Leu,seq:cag)
     gene            complement(8447..8683)
                     /locus_tag="POB34_002469"
     CDS             complement(8447..8683)
                     /locus_tag="POB34_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HDI5919400.1"
                     /translation="MLQRMLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
     gene            8824..9612
                     /gene="fhuF"
                     /locus_tag="POB34_002470"
     CDS             8824..9612
                     /gene="fhuF"
                     /locus_tag="POB34_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HDI5919401.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(9650..10327)
                     /gene="bglJ"
                     /locus_tag="POB34_002471"
     CDS             complement(9650..10327)
                     /gene="bglJ"
                     /locus_tag="POB34_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HDI5919402.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(10285..11010)
                     /locus_tag="POB34_002472"
     CDS             complement(10285..11010)
                     /locus_tag="POB34_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI5919403.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            11629..12399
                     /locus_tag="POB34_002473"
     CDS             11629..12399
                     /locus_tag="POB34_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDI5919404.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            12390..12863
                     /locus_tag="POB34_002474"
     CDS             12390..12863
                     /locus_tag="POB34_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HDI5919405.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            12970..13509
                     /gene="dnaT"
                     /locus_tag="POB34_002475"
     CDS             12970..13509
                     /gene="dnaT"
                     /locus_tag="POB34_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HDI5919406.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            13512..14249
                     /gene="dnaC"
                     /locus_tag="POB34_002476"
     CDS             13512..14249
                     /gene="dnaC"
                     /locus_tag="POB34_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HDI5919407.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            14298..14792
                     /locus_tag="POB34_002477"
     CDS             14298..14792
                     /locus_tag="POB34_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HDI5919408.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            15046..17337
                     /gene="opgB"
                     /locus_tag="POB34_002478"
     CDS             15046..17337
                     /gene="opgB"
                     /locus_tag="POB34_002478"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HDI5919409.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(17477..18499)
                     /gene="lgoD"
                     /locus_tag="POB34_002479"
     CDS             complement(17477..18499)
                     /gene="lgoD"
                     /locus_tag="POB34_002479"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HDI5919410.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            18638..19552
                     /locus_tag="POB34_002480"
     CDS             18638..19552
                     /locus_tag="POB34_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI5919411.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGILTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            19767..21128
                     /locus_tag="POB34_002481"
     CDS             19767..21128
                     /locus_tag="POB34_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5919412.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(21177..>22181)
                     /locus_tag="POB34_002482"
     CDS             complement(21177..>22181)
                     /locus_tag="POB34_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HDI5919413.1"
                     /translation="LVAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQG
                     ELMRTVGDVRNGANAIYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQN
                     AENARQASHLALSASETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNI
                     LALNAAVEAARAGEQGRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLV
                     ESAGETMAEIVSAVTRVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEE
                     SAAAAAALEEQASRLTEAVAVFRIQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF
                     "
BASE COUNT         5387 a         5869 c         5441 g         5484 t
ORIGIN      
        1 atgcggcgtg aacgccttat ccggcctacg gttcggcaca aacctgtagg catgataaga
       61 cgcgtcaagc gtcgcatcag gcattgtgcg ccaactgccg gatgcggcgt gaacgcctta
      121 tccggcctac ggttcggcac aaacctgtag gcatgataag acgcgttaag cgtcgcatca
      181 ggcattatgc gccgactgcc ggatgctgcc tacgaagggt aaactaccca ctaacgctaa
      241 acaacgcata cgtattatta agcaacactt cggcaatctc atcttctggt tccgggcgca
      301 actcgcaaag cacgtcaaac acccgcacag cctgttccgg tcggttaggc tgcccctgaa
      361 agccgttgag cggcatatcc ggcgcgtcgg tttccagcaa caacgatgcc agcggtaatt
      421 ttgcgatgac atcgcgggtt ttactggcgc gtggataggt gatagtaccg cctacgccga
      481 ttttgtagcc cagctgtaca aaccgctcgg cctgttgcag gctgccggaa aaaccgtgaa
      541 ccacgccagt gcacggtaaa tcgtgacgtt taagatgcat cgccagtttg tcgtgtgtac
      601 gccgggaatg aaggatcacc ggcagatcgt agcgtttcgc cagtttcagc tgttcgtcga
      661 gtaaccactg ctgcctctca aattgcggat cgtcgccgaa gagatccaga ccgatctccc
      721 ccaccgccac caccttcgcc ggacgccttt ccagcgcctg ctgtagctga tcaagagaca
      781 catcgctatg tttttccaac atccccggat gcaaccccaa tgcggcatac agcggttgat
      841 atttttccgc taacgcctgc acgcgggcaa aattttccgc ctctgtcgcc ggaacaatga
      901 tcttgcctac gcccgcttgt gccgcgcgtt gcaggctggc ctcttcatcg ccactaaacg
      961 gcgggaaatc aaaatggcag tgggtatcga taaaacggca aatcaagcca gatcctcatt
     1021 gttaaatgtc gggtcgttcg cctgcggagc atcgctcact tcggcaacca gcgtatcgtt
     1081 ggcgactggc gcaggcggaa taacgatcga ttccggcgtc accactggca ccagatggcg
     1141 ggtaagcggc gctttttcag ttaatagctt gccaaccgtg gcgaggaaat aacgaccgca
     1201 taaacgccca agtttatagt cttcacgcag cgccggaatc cgactgccaa gcgcgatact
     1261 atgtaatggt ttcggtggat aaatttcgaa tatccgcagc ttgcccggtg gtttctcaat
     1321 aaattgctga atgtcacgat agctggtttc atgatgctgc accagattga ccagcggctg
     1381 caggctactg tcacccagcc agcgttccat gcgtttgaac cactgcggcg tgtagtacat
     1441 ttgtgacggc acagtgcgga tgacgaccaa cgttttagcg ccctgccttg ccgcctcttt
     1501 aaccggaatc gcatcactga tcccgccatc caagtagtta atgccttcca gcgacactcc
     1561 gctacgataa aagccaggta tcgcactgga ggcgcgaatc acatccagcc agttttgttt
     1621 ggttggtaaa aagtaattag gcgcgtagtc atcctgacga caggcgcaca tataaaacga
     1681 tttgccgctg tcaaacaacc gcgcggcggt atccatttgc aacggcatct ggcttgcggt
     1741 ggcctccacc agccagtcga gatcgataag atttcctcca cggacaaagc gcaatggatc
     1801 gaaaaattcg cgttttgtgg tatagcgcat gatgactttg cgcgcgtaac cgggctgatt
     1861 gcagataaac gccgagaggt tctgcgcccc ggcagatgtg ccgagataaa gatcgaaagg
     1921 attaaactgc gcgcgcataa actcatccag cacgccagcc gtgaaaattc cacgctgtcc
     1981 gccgccttcg cacaccagag ctattcgtcc aggctggaac ggccttagcg acaacggcgc
     2041 aatattacca agcgttacag gtattcgctg ccccacctct gccttcctgt ttttgttgtg
     2101 aaagaatgac aaagtaacgc aatttattat tcgctaaaac cgcaaaagcc agtccgctgg
     2161 actggctttg gataaaattt tcttaatggt ctgaaatcta gggtcgtttt cggcccatga
     2221 acaaactcac caggaacaga ataatcccga cgacaaagac aattttagct gcgcctgcag
     2281 cggtaccggc cagaccacca aacccaagtg cggcggcgat taacgcgata accagaaata
     2341 tgatgcccca acgaaacata agcgtctcct ttaccatagt taatgtcacc gcttgatatg
     2401 agcgtgaaaa tcactcaatg cgatattttt agtgtggtgc acatcgcgcc tcccgacaaa
     2461 gttcgggagg acgaattacg acgaattact tagttttcag atcattttta acgcttttca
     2521 caccatctac cgctttggcg atactttcag cacggtcgct ctgtgcctgg gaatcgacgg
     2581 taccggagag ctgaaccacg ccgtcggtgg tttcaacttt cacatgacgg gaagggacga
     2641 tatcgtccgc cagcagtttg gctttgattt cactggtggt ggcggtgtca cccgcgtagc
     2701 ccttcacaga gccttcttta gcgtcgcgaa cgtgcagttt gtcgctgaca gaggtcaccc
     2761 cttcaacgcc tttcgccact ttcactgcct cttcggcctg ggcctggctt tcaacgaaac
     2821 cgctcagggt cacgactttt tgatcggttt ttacagagat atcggtgctc ttgatgttgt
     2881 catgatccac cagggccgcc ttcactttcg cggtgatggc gctgtcatcc atgaaattac
     2941 cgactttatt catagagcta tcgacttttt gccctgcgct ttcattggta gtctgtgcgt
     3001 tgttttccgc gtaggcagag ccggtcgcga cggcagaggt caacattaca gccagcagag
     3061 ttttcgaaat cttcagtctt gtcatagtca tcgatttatt cctgtatgtt tgctcgtaat
     3121 ttgagcctgg caacacgagg ttgcattgct gaatagggag agacttcacc ctctacagaa
     3181 gtcaatggtc gccatcacaa aagcgatggg tgatgaataa cgaccattac agcctctgaa
     3241 tcagttatta atatcggtag aatgacaatc gacggcttta gatactgata tctacgcatt
     3301 gaacggtatt taacgccgtc agaaatgtca ttactttgtt aaatatagat cacaattttg
     3361 aaaccgctcg ggatatcagc gaaaacataa gcaaaagtga atgttttaag aacattccgt
     3421 aagcggctaa taaggaaggg aaattgacag ggcgaggcgg ttgccgcgcc ctggaggcaa
     3481 gaattaatgc tcgcgggtct ggtggaactg aacgtccgga taacgttcct gtgccaggcg
     3541 caggttaacc atgctggtag cgatgtaagc gagattatcg ccgccatcaa gcgccagttg
     3601 gctttcgttc ttacgcttga actcttcaaa tttcttcgcg tccgcacatt ctacccaccg
     3661 ggcggtggca acgttgactg attcatatac tgcttcaacg ttgtattcgc tcttcaggcg
     3721 cgataccact acatcaaact gcagcacacc aaccgcacca acgatcaggt cgttgttgga
     3781 gatcggacgg aacacctgca ccgcgccctc ttcggaaagc tgtaccagcc ctttgagcag
     3841 ctgtttttgc ttcagcggat ctttcaggcg gatacgacgg aacagttctg gtgcgaagtt
     3901 cggaataccg gtgaacttca tcatctcacc ctgggtaaag gtgtcgccga tctgaatggt
     3961 gccgtggttg tgcaggccga ggatatcgcc aggatacgct tcttcaacgt gcgaacggtc
     4021 acccgccata aaggtcagcg cgtcagagat caccacgtct ttcgcggtgc gcacctggcg
     4081 cagcttcatg cctttttcat atttaccgga taccacgcgc ataaacgcca cgcggtcgcg
     4141 gtgtttcggg tccatgttgg cctgaatttt aaatacgaag ccggtaaatt tgtcttcgct
     4201 cgcttgtacg gtacgggtat cagtctgacg cggcatcggc gcaggtgccc actccaccag
     4261 gccatccaac atatgatcga cgccgaagtt acccagcgca gtaccgaaga ataccggagt
     4321 gatttcgccc gcaaggaaca gctctttgtc gaactcgtta gacgcgcctt taaccagttc
     4381 cagttcgtca cgcagctgct gtgccagatc ttcaccaacc gcagcatcga gatccgggtt
     4441 attcagccct ttaacaatgc ggacttcctg aatggtgtgc cctttaccgc tctgatagag
     4501 ataggtttca tctttataaa ggtggtaaac gcctttaaac agcttgccgc agccaattgg
     4561 ccaggtgatc ggtgcgcagc caattttcag ctcgttctca acttcatcga gcaattccat
     4621 cgggtcgcgg atatcacggt caagtttgtt cataaaggtg aggatcggcg tgtcgcgcag
     4681 acgggtaact tccatcagct tacgggtacg atcttcaaca ccttttgcgg cgtcgataac
     4741 catcaggcag cagtccaccg ccgtcagggt acgataggta tcttccgaga agtcttcatg
     4801 ccccggggtg tcgagcaggt taaccaggca atcgtgatac ggaaactgca tcacagacgt
     4861 agtaatggag atcccacgct gcttttccat ctccatccag tccgacttag cgtgctggtt
     4921 ggaaccacgg ccttttactg taccggcggt ctgaatggcc tgtccgaaca gcagcacctt
     4981 ctcggtgatg gtagtcttac cggcgtccgg gtgagaaata atggcaaaag tgcggcgctt
     5041 cgccacctct tgcaaataag gagacaacgt cataattctt tcttcttgag taagcggcat
     5101 cgcgccgcgc ttgttgaaaa cgaaaaattg cggctatttt acccatcaac gggggggagg
     5161 caatcagtgt ttacacagga gctgctccag ttcatgcaac gaagaaacgg tccaggtggg
     5221 cgcgatgcct tctggttgct cgcgatggtg tgcattcagc cagcaggtcg caagcccggc
     5281 gttgatgcca ccgagaatat cggactcggc agtgtcgcca accatcagca cgcgtgaacg
     5341 gtcaggattg cccgcctgtt ccagcgcata atcgaaaatt ttcttattcg gtttagcaac
     5401 gccaacttct tcggaaatca ccagcaaatc gaagtaatca cgcaggcccg tccgttccag
     5461 acgcacttgc tgcaaggcgc taaaaccgtt ggtgatgatg ccgattttag cgttgccacg
     5521 aatggcgtta agcagagaaa ccgcgcccgg caacggcgta cagatttccg ccatcgcatt
     5581 aataaaggca tcgttaagca agcctgcttc aactttcagc cgttcggccc agctctcaaa
     5641 ccgcccgtgc tgaagctgta atgaagtgat cgcgccgttt tgataatcca cccacagtgg
     5701 cttgttaacg gcctgatagt cctgaaaatc ttcagcggta aaggtgacgc tgtaatcaag
     5761 aaacatccgc tgcaggccgg tgaatgagtc aaaggtaaac agcgtttcat cggcatcaaa
     5821 gaaaatccag tcccacttca ttattccacc ttgtcttaca tactgattgg caacgccatg
     5881 atgatggcgt cttcgcgacc gtccgtggtg gggtagtaat tgcggcgaat cgtcgcctcg
     5941 ttaaagccta aactttcgta cagggcaatg gcggcagcgt ttgaagcacg gacttccagc
     6001 catagtgtcg ccacgccgcg tttttccagt tcgtcgatca gatgttccag cagcaccctt
     6061 cccaatccct gacgctgata gtcaggatcg accgcaatat tgaacaatgt agcttcatcc
     6121 agcaccactt gcgtaatcgc aaacgccgcc attttgccgt tttgcgttaa ctgaaagttg
     6181 agataacgct cgccctggtt gctggcaaag gttttttcac tccacggaaa ggcgtgggcg
     6241 cgttgttcaa tgtggtaagc cgctggtaaa tcagtcgttt cgagggaaga aatcgtgttc
     6301 atatgtgcaa atctgttgcc ataacgcggc gcgtgccgtt gggtttgccc gtaattcggt
     6361 gagcgccggt gatgccacct gagcgccttc cagtgatagc ggttcgtcag tacccaaccg
     6421 ccagctgttg cagcgactgc cttgcggcag catcgcgatt ttttctggcg tcagttgcag
     6481 cacttgatcg gggctgacgg ttaatgcgcg cagaacatcg ctcactaaag gatcggtcag
     6541 ggcgggaaga tcgtttgcca ccatcaccag acggacgtgt gccgggatcg caatggcaat
     6601 ctccccctgc aacgcgccag ggcgacgcag cgaccactgg gtaatgccca gttgctgtaa
     6661 ctgccagtct cgtcgggatg tcatagcgaa tcgctcctgt tgtcaggggc gcaaatatag
     6721 caaattcgtc gataccgcgc caacatatgg ctataatcgc cgccagtatc aattgaggag
     6781 cattccatgt ctgcatttac cccggcaagt gaagtcttgc tgcgtcacag tgatgatttc
     6841 gaacaaagcc gtattctgtt tgccggagac ttacaggatg acctgcccgc gcgtttagat
     6901 accgcggcca gccgtgctca tacccagcaa ttccaccact ggcaggtatt aagccgccag
     6961 atgggggata acgcccgttt cagtctggtc gccacggtgg atgacgtcgc agattgcgat
     7021 acgctgattt actactggcc gaagaacaaa ccggaagccc agttccagtt gatgaattta
     7081 ctttctctgc tgccagtggg gacagatatt tttgtcgttg gcgagaaccg cagcggcgtg
     7141 cgcagcgccg agcagatgct ggcagattat gcgccgttga ataaagtcga cagcgctcgt
     7201 cgctgtggcc tctattttgg tcgtctggaa aaacagccgg tatttgatgc cgataaattc
     7261 tggggcgaat acagcgtcga tggcctgacg gtcaaaacgc tgcctggcgt gtttagccgc
     7321 gacggtctgg atgtcggtag ccagttgctg ctctcgacgt taaccccgca cacgaaaggt
     7381 aaagtgctgg atgtcggctg tggcgcgggt gtgctttcag ttgcctttgc gcgccattcg
     7441 ccgaaaattc gtctcacctt gtgcgatgtc tctgcgccag cggtagaagc cagccgcgca
     7501 acacttgcgg ccaacggtgt tgaaggtgaa gtctttgcca gcaacgtctt ttccgaggtg
     7561 aaaggtcgtt ttgatatgat catctccaac ccgccgttcc acgatgggat gcaaaccagc
     7621 ctggatgcgg cgcaaacgct gattcgcggc gcggtgtgtc atcttaatag cggcggcgag
     7681 ctgcgaattg tagcgaacgc cttcctgcct tacccggacg tgctggatga gacatttggc
     7741 ttccatgaag tgattgcgca aaccgggcgt ttcaaggtgt atcgcgccat tatgacccgc
     7801 caggcgaaga aaggttaatt atctcgccga ataccgtgtc ggatgcggcg tgaacgcctt
     7861 atccgaccta caaaacctgc acgttagccc ttcgtaggcc agataagacg cgccagcgtc
     7921 gcatctggca tctgcccccg catgcctacc aaacctgcac gctagtttcc tgatggacat
     7981 ttttccagca attacacctc cggcgataat taactattga cgaaaagctg aaaaccacta
     8041 gaatgcgcct ccgtggtagc aattcttttt aagaattgat ggtatgcgaa ggtggcggaa
     8101 ttggtagacg cgctagcttc aggtgttagt gtccttacgg acgtgggggt tcaagtcccc
     8161 cccctcgcac caaaaaccac gttgatattg ctcgcactgg gcgaaggtgg cggaattggt
     8221 agacgcgcta gcttcaggtg ttagtgttct tacggacgtg ggggttcaag tcccccccct
     8281 cgcaccaaac gaggcgatat caaaaaaagt aagatgactg tgcgaaggtg gcggaattgg
     8341 tagacgcgct agcttcaggt gttagtgtcc ttacggacgt gggggttcaa gtcccccccc
     8401 tcgcaccaat tatctttact tcctttcttg tttcttcctt gatttttcat ccctgattca
     8461 aattcattat catcagcatg atgccagcaa taagcgccag gcacgatggt agcaaaacgt
     8521 aatgccgtaa ctgtttgtga tacagcatca ccggcgtcaa tactaatccc atcagaatga
     8581 caatccgcca ctgatccggc gataaatccg catacatcag ccctgcaatc agcaatcctg
     8641 gcagcaacac tccccagcca ctgcccagca tacgttgcaa catggtttca tctctttcat
     8701 tgataatgat aaccaatatc atatgataat ttttatcatt tgcaagccag agaaatccct
     8761 tgctatcggg taaacctatc gctaggatta gcaatcatta tcatttagat tactatcccg
     8821 attatggcct atcgttccgc accgctctat gaagatatca tctggcgaac gcatctccag
     8881 ccgcaggatg ctgggcttgc acaagccgta cgtgcgacga tcgccaaaca tcgtgaacat
     8941 ttgctcgagt ttatccgcct ggatgaaccc gctccgctta acgccatgac gctggcgcaa
     9001 tggtcatcac cgaatgcgct aagttctcta ctcgcggtct attccgatca tatctatcgc
     9061 aaccaaccaa cgatgatccg cgagaacaaa ccgctgatct cactatgggc acaatggtat
     9121 atcggcctga tggtgccacc attaatgctg gcgctactga cgcaggaaaa agcgttagat
     9181 gtgtcgccgg aacatttcca tgctgagttt cacgaaaccg gacgcgtcgc ctgtttctgg
     9241 gtcgatgtgt gtgaagataa aaacgcaaca ccacattcgc cgcagcagcg aatggaaacg
     9301 ttaatcagcc aggcgctggt tccggttgtg caagcactag aagcgaccgg agagatcaac
     9361 ggcaaactta tctggagtaa taccggttat ttgatcaact ggtatctcac tgagatgaag
     9421 caactgctcg gcgaggcgac tgttgaatcg ctgcgccatg ccctcttttt tgagaaaacg
     9481 ctcacgaacg gtgaagataa tccactctgg cgtaccgtgg tactgcgcga cggcctgctg
     9541 gtgcgccgca cttgctgcca gcgttatcgc ttaccggatg tgcagcaatg tggcgattgt
     9601 acgctgaaat gaaagcactg ccggggaagt aaacccggca tcatgcggat taatagggat
     9661 gcaacacatt acttgtttcg caatgccgca tacataacag aatatctgcg gcctccaaca
     9721 accctgcgtc agaactgacg cccagtttcg acatcacatt aaatttatgt gcacggatcg
     9781 ttttgatatt gcgtttaagc tgctcggcaa tttgtgtcat tgagtagcca cgcgacataa
     9841 agcgcaatat ttctctctcc gtcgggctta acgtccggct ttggtttatg taccactgat
     9901 tgttcagtcg gtcggtcgcc tgacgtacac catttaatga caaaaagagt tcctgatgaa
     9961 aaatctccag cgttgacgct ttacttaata caccgtccag cggtgatggc gacaatgaac
    10021 caatcagccg agcttcaata tcatcatccg caataactaa acgccgggta cgcggaaact
    10081 taatcgccag ttcagtcagg caagataatc cctctctgcg ctcacttctc atggcagaaa
    10141 aagaaaaaat gaccgccgca aacgagatcc gcgacatggc atcctgaaat gactcctgac
    10201 tggtataggt atggagctta taagggttac ccgcaaactt tctgaataaa ctctcaatac
    10261 cgatactact catgacgcat ttttctatga gtgcgacatt tctcttctta attcggctgt
    10321 gttccattct gcggcatctc cataaacaga aggcaagtta agttctcttg caccctgcgc
    10381 gctatttatc catgcataca tttcagcgtt gctgtgaatt gccagtcgac gcatcgcact
    10441 gtttttttgg gcgctgatag ttttattact tttcttaagc aatgaagcta tctggttgat
    10501 gccccatcct ttacctaata gccgcagtac cttgcgctct gaaagagtga gaatgacgga
    10561 gcgatagcta aaatcatgag tttcagtcat tgccggggag gtcagcatgg cgctgatacg
    10621 ctctgcgtgc gtattgccgg aacgtacggt cttgaccaga ttctcaatgg gttcaacatc
    10681 agacaataac gtcgccgtag ggcacatgag cagttctact gcctgggaat accaggagcg
    10741 tgacaccatg aaaacccagt gaatttcccg atattgtgag attaaagaat aataatgttc
    10801 acaaacagaa cgtggatctt cactttcacc ggctaaatca gcaatgacta atccggaacg
    10861 acgtaattgt aataaggtaa ggtcctccgc agaggcgctg gatattattt catattcagg
    10921 gaagtgagtc ctcatgacct cttttaaccc tgcttgcata acaggtattt tactgataac
    10981 aattccattt ttgcagcatc ctggcaacat atttcctcca catccactgt ctaaaaatta
    11041 tgatggttta ttcaggatat tcattcctta aatgtttcca gcaaattaat agattaagcg
    11101 aataaattat ccttagcatt cactttcata tcagcatact cctgctgcac aacaactcca
    11161 ctgcatgtgt cagaaggtta gcatcaaatg ataacctggt gaaatagcct ggagacattt
    11221 aaaatatcta cattaagttg ttaggaatac ttaatgaaaa acagaaatca cattcattta
    11281 atttataatt aaatgatatc ccttcccgca gaatgaaaaa tcacacaacc aatatttatt
    11341 caatgaaatc tgatagtttt aacttgttaa cattaaaaaa cgtgcccggt tatctattat
    11401 cctgattcgt aaaggccaaa caataaattc attatgatga taatattcat gtgatagttt
    11461 gtttgcggcg agagataatt cgctttttat caccgagctt ctcccgataa ccaggattaa
    11521 tcagcgcaat tttgatctta acctaccgca aaacaaccgt gcttcatgat acaaatggat
    11581 tctcattatc tgaacaatac gcagcacgtc tatgacaaag ggcgagttat gcaaactgag
    11641 caacagcgag ccgtaacacg gctttgtatc cagtgtggat tatttctttt gcaacatggt
    11701 gcggaaagcg cgttggttga tgagctttcc tcacgactgg gtcgggcgct gggaatggac
    11761 agtgtcgaaa gttctatctc ttcgaacgcc atagtgctga caactattaa agatgggcaa
    11821 tgcctgacat cgacacgtaa aaatcacgat cgcggcatta atatgcatgt ggtgactgaa
    11881 gtccagcaca ttgtgattct tgcggagcat catctgctgg attacaaagg cgtagagaaa
    11941 cgatttagcc aaattcagcc attacgttac ccaagatggc tggtagcctt aatggttggc
    12001 ctttcttgcg cctgtttctg taaactcaat aacggtggct gggatggtgc cgtcatcacc
    12061 ttctttgcca gtacgaccgc gatgtatatc cgccagctgc tggcacaacg tcatcttcat
    12121 ccacagatca acttttgcct taccgctttc gccgccacca ccatttccgg attgcttttg
    12181 caactcccca ctttcagcaa tacccccacc attgcgatgg ccgccagcgt tctgctgctg
    12241 gtgccgggct ttccgttgat taatgccgtc gccgatatgt ttaaaggcca catcaatacc
    12301 ggactggcac gctgggcgat cgccagtctg ctgacactgg ctacctgcgt cggcgtggtg
    12361 atggcactga cgatttgggg gctacgcgga tgggtgtgat cgaatttctg tttgcattgg
    12421 cgcaggatat gatcctcgcc gccattcctg cggtcggctt tgcgatggtg ttcaacgttc
    12481 ccgtgcgggc gttacgctgg tgtgcgctgc ttggctcgat aggtcatggt tcccgaatga
    12541 tcttgatgac cagcgggttg aatattgagt ggtcaacctt tatggcttct atgctggtcg
    12601 gcaccattgg tattcaatgg tcgcgctggt atctggcgca tccgaaagtg tttaccgtgg
    12661 cggccgttat ccctatgttc ccgggcatat cggcttatac cgcaatgatt tcggcggtaa
    12721 aaatcagcca gctaggttac agcgaaccgt tgatgattac cctgttaacc aactttctta
    12781 cagcttcatc gattgttggt gcgttatcca tcggtctttc cattcctgga ttatggttgt
    12841 accgcaagcg ccctcgcgta taaaattgcc tcgcttaagc acacggatga gagacagcct
    12901 cctctcctcc gtgtgttact ataaaagtta tctcccttct cgttcatcgt tccatatttg
    12961 agaaacagta tgtcttccag agttttgacc ccggacgtcg ttggtattga cgccctggta
    13021 cacgatcacc aaaccgttct ggcaaaagct gaaggcggtg tggttgccgt atttgctaac
    13081 aatgccccgg cgttttatgc cgtcacgcct gcacgcctgg ctgaactgct ggcgctggaa
    13141 gaaaagctgg cgcgtccggg aagcgatgtc gctctggacg atcaactcta tcaggaaccg
    13201 caagccgctc ccgttgctgt acccatgggg aaattcgcca tgtatccgga ctggcaaccc
    13261 gatgccgact ttatccgcct ggcggcgcta tggggcgtgg cgctaagaga gccggtaacc
    13321 accgaagaac tggcctcatt cattgcctac tggcaggcgg aaggtaaagt ctttcaccat
    13381 gtgcagtggc aacaaaaact ggcgcgcagc ctgcaaatcg gtcgtgccag caacggcgga
    13441 ctgccgaaac gagatgtgaa tacggtcagt gaacctgaca gccaaattcc accaggattc
    13501 agagggtaac gatgaaaaac gttggcgacc tgatgcaacg cctgcaaaaa atgatgcctg
    13561 cccatatcaa acccgcattc aaaacgggtg aagaactact ggcctggcaa aaagaacaag
    13621 gggcgatccg ctccgccgct ctcgaacgtg aaaatcgggc gatgaaaatg cagcgcacct
    13681 ttaaccgctc cggtattcgt ccactgcatc agaactgctc ctttgagaac tatcgcgttg
    13741 agtgtgaagg gcagatgaat gcgttaagca aagcgcgcca gtatgtcgaa gagtttgacg
    13801 gcaacatcgc cagctttatc ttttctggta agccaggaac cggcaaaaac catctggcgg
    13861 cggcaatctg caacgagctg ctgctacgcg gtaaatccgt attgatcatc accgtggccg
    13921 atattatgtc ggcgatgaaa gataccttca ggaatagcgg taccagcgaa gaacaactgc
    13981 ttaacgatct gagcaacgtt gatctgctgg tgatcgatga gatcggcgtg cagaccgaat
    14041 cgaaatacga aaaagtgatc atcaaccaga tcgtcgatcg ccgctcttct tccaaacgcc
    14101 caaccgggat gctgaccaac agtaatatgg aagagatgac aaagttgctg ggcgaacgcg
    14161 tgatggaccg tatgcgcctg ggtaacagtt tgtgggtgat cttcaactgg gatagctacc
    14221 gtagccgggt aacgggtaaa gagtattaat ccccgtttct ggcggtatac tgcgacacaa
    14281 ttccaccttg agtaatgatg atgaaaacta ttaaacacct tctgtgctgt gccattgccg
    14341 ccagcgcatt aatttccacc ggggtgcatg ctgcgtcctg gaaagatgcg ctctccagcg
    14401 ccgccagcga acttggcaac caaaacagca cgacacagga aggcggttgg tcgctcgcgt
    14461 cattaactaa cttgcttagc agcggaaacc aggccttaag cgcagataac atgaacaatg
    14521 ccgcaggcat tctgcaatac tgcgcgaagc aaaagctggc ttcggtaacc gatgccgaaa
    14581 acatcaagaa ccaggtgctg gaaaagctgg gcctgaacag tgaagagcaa aaagaagaca
    14641 ccaactatct ggacggtatt cagggtttgc tgaaaaccaa agatggtcag caactcaatc
    14701 tggataacat cggaacaact ccgctggcag aaaaggtgaa aaccaaagcc tgcgatctgg
    14761 tgttaaagca ggggctgaac ttcatttcct gaaccgcgaa acgccgcgtt ttagctgtta
    14821 cccggctgac gcggctctct cctgcccttt tccctgctcc tggacggttt tccccctgtt
    14881 acacgggaag cgaccgcgat caaaaaagtc tgtttataaa ctaattccta acaacatcgc
    14941 atttttttga cactagaatt gcagcaatat ggcgacgcca ttacattgtg cttctcaaaa
    15001 agcttaaacg ttggctaaac ggcccggttt agtgaggatc gcccgttgtc agaactactc
    15061 tctttcgccc tttttctcgc ctctgtgctg atttacgcat ggaaagcggg acgtaacacc
    15121 tggtggtttg cagccacatt aacggtgctg gggctatttg tcgttttaaa tatcaccctg
    15181 tttgccagcg actattttac tggcgatggt attaacgacg cggttctcta taccttaacc
    15241 aacagcctga ccggtgctgg cgtcagcaaa tacatcctgc cgggtatcgg cattgtgctg
    15301 gggctgacag cggtgttcgg tgcgctgggc tggatcctgc gccgtcgtcg ccatcatccg
    15361 caccattttg gttacagcct gctggcgctc ttactggcgc tgggttcggt ggacgccagc
    15421 ccagcatttc gtcagataac ggaactggtg aaatcccagt cacgcgatgg cgacccggac
    15481 tttgcggctt attataaaga gccgtcgaaa actatccctg acccgaaact caacctggtt
    15541 tatatctacg gcgaaagtct cgagcggacc tattttgata acgaagcttt cccggatctc
    15601 acgcctgaac tgggcgcgtt gaaaaatgaa ggcctggatt tcagccacac gcagcagctg
    15661 ccaggaacgg attacacgat tgcgggcatg gtggcttctc agtgcggcat accgctgttt
    15721 gccccctttg aaggcaacgc ctccgcctct gtctccagct tcttcccgca gaacatctgt
    15781 ctgggcgata tcctgaaaaa ctcgggttat cagaactatt tcgtgcaggg cgcgaatctg
    15841 cgttttgccg gtaaagatgt gttcctgaag tcgcatggct tcgaccactt atacggctca
    15901 gaagagctga aaagcgtggt ggccgacccg cactatcgca acgactgggg attctacgac
    15961 gataccgttc tcgatgaagc gtggaaaaag tttgaagagc tttcccgctc aggtcagcga
    16021 ttctcactgt ttaccctgac agtcgatacc catcacccgg atggttttat ctcgcgtacc
    16081 tgtaaccgca aaaaatatga ttttgacggt aagccgaacc agtcattcag cgcggtaagt
    16141 tgcagccagg agaatatcgc ggcgtttatc aacaaaatca aagcgtcacc gtggtttaaa
    16201 gataccgtca tcgtcgtctc ttctgaccat ttagcgatga acaacacggc gtggaaatac
    16261 cttaataagc aggatcgcaa taacctgttt tttgtcattc gtggcgacaa gccgcagcaa
    16321 gagacgctgg cagtgaagcg taacacgatg gataacggcg cgacggtgct ggacattctc
    16381 ggtggcgata actatctcgg acttggtcgt agcagtttat ccgggcagtc aatgtcggaa
    16441 atcttcctca atatcaaaga gaaaacattg gcgtggaagc cggatatcat ccgcctgtgg
    16501 aaattcccta aagagatgaa agagttcacc atcgaccagc agaaaaacat gattgccttc
    16561 tcgggtagcc atttccgttt gccgctgctg ttgcgggttt cagacaaacg cgtggaaccg
    16621 ctgccggaaa gcgaatactc agcaccgctg cgtttccagc tggccgattt cgctccacgc
    16681 gacaatttcg tctgggttga ccgttgctat aagatggcac aactctgggc tccggaactg
    16741 gcactctcca ccgactggtg tgtctcgcaa gggcagcttg gcggtcagca aattgttcag
    16801 catgttgaca aaacaacatg gcagggcaaa acggcattta aagatacggt catcgacatg
    16861 gcgcgttata aaggcaatgt cgatacgctg aaaattgttg ataacgatat tcgctacaaa
    16921 gccgacagtt tcatcttcaa cgtcgccggt gcgccagaag aggtgaaaca gtttagtggg
    16981 atttcccgtc cagagtcgtg gggccgctgg tccaacgcgc agctgggcga tgaagtaaaa
    17041 atcgagtaca agcatccgct gccgaagaaa tttgacctgg tgattaccgc caaagcatac
    17101 ggcaataacg ccagccgtcc tattccggta cgcgtaggca atgaagaaca aactcttgtg
    17161 ctgggcaatg aagtgaccac caccacactg catttcgata acccaaccga tgccgacaca
    17221 ctggtaattg tgccgccgga acctgtctca accaacgaag ggaatatcct cggacactcg
    17281 ccgcgtaagc tcgggatcgg catggtggaa attaaagttg tggaacgtga agggtaattt
    17341 ctgactgatg tgtaggtcgc ataaggtgcg acctacacat cttctccggc ctacaaaaca
    17401 tcattgagaa acagaaaaaa aacagcgccc aacacttccc cggacgctgc atggcaactt
    17461 taataatctc gccttatcag aaagtaatta cacccttaat caactcacga ttgttaatca
    17521 catcgcgctc gtaggtttct gccagcgtgg cgaacggata gcgatgggtt aacatcatgt
    17581 cggcagtgat ttttccttcc gccatcagtc gaccgacttt agcaaaatct tccggcgtgg
    17641 cgttgcggct gcccatcatc gtcgtttctt ttttatggaa ttccggatcg gagaactgca
    17701 actcaccttt aaacaggccg acaaatacca ccgtgccgcc gtgacgaatc aaattcacgg
    17761 tgttattcat cgcatgttga ttacctgtcg cgtcgatcac tttctgcgcc agcgaaccac
    17821 caaactgcgc ccgtagctgc gcgtcaaaat cttcggctga cgggtccagt aaaggtaatt
    17881 ccagacgcgt tgccacatgt tcacggcgcg ccggactggt atccgccacc accacctgtg
    17941 cgccatcggc tttagcgatt gccgccgcgc ccagaccgat tggccccgcc ccgaccacca
    18001 gcacctgctc gccgggagca atggctgcgc gacgcaccgc atgagcgcta atagcgaaag
    18061 gttcaatcaa tgcggccgcc tgcgggtcaa taccgtctgc gggcaaaatg ttcgccaccg
    18121 gcacgctcaa atactcacta aaaccgccat cctgatgcac gccaatgact gaaatttttt
    18181 cacagcaatt ggtacgcccg cttttacacg ccgggcattg ctgacaggca acataaggga
    18241 tcacagcaac ttgctgacca tttttaagat cagcaatatt tttacccagc ccaacaatct
    18301 ccccacatat ttcatggcct aaaacacgtg gataactaaa aaatggttga tttccacccc
    18361 aggcatgaat atcggtaccg caaatcccga cagacttaat ttttattaat gcttcattgt
    18421 cacccggaat aggtatctcg cgttgtttcc agactaattc tttcggctgc tggcaaatta
    18481 aaacattcat cgtagacatg attcggctct ccattttttg ttgccttaga tatcaatgaa
    18541 aatgaaaaat ggcagcggaa cggaaaatct gttttgtgaa aacacacgca taaaaatgtt
    18601 ttaaatcggg ttttaatgga ctcacaagga ggaaaatatg agtcgttcac aaaatttacg
    18661 ccacaatgtg attaaccagg tcattgatga tatggcgcgg ggtcatatcc cttccccact
    18721 tccatcgcaa agtgcgctgg cggaaatgta caacatcagc cgcacaacag tgcgtcatat
    18781 tctcagccac ttacgcgaat gcggcatctt aacgcaggtt ggaaacgact atgttattgc
    18841 gcgtaaacct gaccatgatg acggtttcgc ctgtactacc gcttcaatga gtgaacaaaa
    18901 caaagtgttt gagcaggctt ttttcacgat gataaaccag cgtcagttac gccctgggga
    18961 aactttttct gaactgcaac tggctcgggc cgcgggtgtc agcccggtgg ttgtaagaga
    19021 atatctttta aaattcggcc gttataatct tattcaaagc gaaaaacgcg gccagtggag
    19081 catgaagcaa ttcgatcaat cctacgccga gcagctgttt gagctacgcg aaatgctcga
    19141 aacacattca cttcagcact ttctcaacct gcctgatcat gatccccgct ggctgcaggc
    19201 gaaaaccatg ctcgaacgtc atcggctact gcgtgacaac atcggcaata gctttcgcat
    19261 gttctcgcaa cttgacagag attttcactc cttactgctt tcagctgccg acaatatctt
    19321 ttttgatcaa tcgcttgaga tcatctcagt catctttcat tttcactatc aatgggacga
    19381 aagcgatctt aaacagcgca atatcattgc cgtcgatgag catatgacca ttctcagcgc
    19441 tctgatttgc cgcagtgacc ttgatgccac ccttgcgctc cgtaaccatt tgaattcagc
    19501 taaacaatca atgattcgat ccattaatga gaacacgcgt tatgctcatt aagtacggat
    19561 taaaaacagc aaactattag cgggtgcacg gttttatcaa aagataaata ccgggaaatt
    19621 cccgcttacc tatgctcaca atcagtcgac taaaaatttt tttgtgagcg gtagtaaagt
    19681 cctaaaactt taacctgccg ttaatgtcaa ccttgcgtaa ttcacaatta cgtaaacaat
    19741 taaaattata aatacaggag tccggcgtgg aaaaagaaaa tatcaccctc gatccgcgtt
    19801 cttcatttac tccatcttcg tcggcagata ttcccgtgcc accagatgga ttagttcaac
    19861 gcagtacccg aattaaacgc attcaaacca ccgccatgtt gttattattt tttgcggcgg
    19921 taatcaatta tctcgaccgc agttcgctgt cggtagcaaa tttaacgatt cgtgaagaat
    19981 tgggattaag tgccaccgaa atcggcgctt tgctctccgt gttttcactc gcttacggga
    20041 ttgcgcaact tccttgcggc ccactattgg atcgtaaagg cccacgcctg atgctgggac
    20101 tggggatgtt cttctggtca ctgttccagg caatgtctgg catggtgcac aactttacgc
    20161 agttcgtgtt ggtgcgtatc ggtatgggga ttggtgaagc gccgatgaac ccatgcggtg
    20221 taaaagtcat taacgactgg ttcaacatca aagagcgcgg acgcccgatg ggcttcttca
    20281 acgcagcttc taccattggc gttgccgtaa gcccaccgat tctggcggcg atgatgctgg
    20341 tgatgggctg gcgcgggatg tttattacca ttggtgtact ggggattttt ctcgccatcg
    20401 gctggtatat gctctatcgc aaccgcgagc acgtagaact gactgccgtt gagcaagctt
    20461 atctcaatgc aggtagcgtc aatgcccgcc gagatccgct cagttttgcc gaatggcgca
    20521 gcctgttccg taaccgtaca atgtggggaa tgatgctcgg attcagtggc atcaactaca
    20581 ctgcgtggct gtatctggcc tggcttcctg gttacctgca aacagcctat aacctggatt
    20641 taaaaagcac agggttgatg gcggctatcc ctttcctgtt tggggctgcc gggatgctgg
    20701 tcaacggtta cgttactgac tggctggtca aagggggaat ggctccgatt aaaagccgta
    20761 agatctgcat tattgccggg atgttctgtt ctgccgcctt tacgctgata gtaccacaag
    20821 cgacaacatc catgacggcg gttctgctga ttggcatggc actgttctgt attcactttg
    20881 ccggaacatc ctgctggggc ttgatccacg tcgcagttgc ttctcgcatg actgcgtcgg
    20941 tgggcagtat ccagaacttt gccagcttca tctgcgcctc ttttgcgccg atcattactg
    21001 gttttattgt tgataccacc cactcattcc gtctggcact aatcatctgc ggttgcgtca
    21061 ccgcagcggg ggcactggcg tacatcttcc tggttcgtca gccgatcaac gacccacgta
    21121 aagattaatc tccttatgcc cgataacatt ttgcttatcg ggcattttca tggcgattaa
    21181 aatgtttccc agttctcctc gctatctgcc acggccattt tacgcggcgc agctggcgtc
    21241 acggttttta ccacagccga tgtttcacgc tgctgttgct gaatccggaa cactgccact
    21301 gcttcggtca ggcgactggc ctgctcttcc agcgcggcgg cggcagcggc agactcttcc
    21361 accagcgcgg cgttctgttg agttacccgg tccatctcag caaccgctaa gccaacctga
    21421 tcgataccac ggctctgctc atcagaagca gaagcaattt cgcccataat gtccgtcacg
    21481 cgggtcacgg cgctgacaat ctccgccatt gtttccccgg cgctttcgac cagcgtagag
    21541 ccaacatcca ctttccccac cgagtcttca atcaggcttt taatttcacg agccgcctgg
    21601 gcgctgcgct gggccagatt acgcacttct cccgcgacca ccgcaaaacc gcgcccttgc
    21661 tcacccgcac gcgcagcctc aaccgccgcg ttcaaagcca gaatattggt ctggaaggca
    21721 atgccgtcaa ttacgctgat aatatcggcg attttctgcg aactggtgga gatatcgcgc
    21781 atagtctgca ccacgttatc taccactttg ccgccgcgtt gcgccgtttc agaagcactt
    21841 aacgccagat ggctggcctg gcgcgcattc tcggcgttct gtttcaccgt tgcggtcagt
    21901 tgctccatgc tggctgccgt ctcttccagc gaagcggcct gttgctcggt gcgcgaagag
    21961 agatcgttat tgccggtggc gatttcgctg gcaccgctat agatggcatt ggccccgttg
    22021 cgcacatcac cgacggtacg catcagctct ccctgcatat ggcgcaaact ctctgccagt
    22081 tgccccatct cattagagcc atccacctca atcggtttca ccagatcgcc gcctgcaata
    22141 tgacgaatgc tgtcaatcag gcgattcatt ggcgctacca g
//
LOCUS       DAMZET010000064        22007 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.064, whole genome shotgun sequence.
ACCESSION   DAMZET010000064 DAMZET010000000
VERSION     DAMZET010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.064"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..931
                     /locus_tag="POB34_002483"
     CDS             <1..931
                     /locus_tag="POB34_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HDI5919414.1"
                     /translation="RLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFGIADPNFKI
                     QLPPFALSVAVMCLVLGMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSHSLSGELHW
                     QWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTRYRRSFVAT
                     GFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVPALTRLFCS
                     IPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMALPPVYLQD
                     LPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            953..2098
                     /gene="glxK"
                     /locus_tag="POB34_002484"
     CDS             953..2098
                     /gene="glxK"
                     /locus_tag="POB34_002484"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HDI5919415.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMEECDPRLANSHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(2326..3111)
                     /gene="allE"
                     /locus_tag="POB34_002485"
     CDS             complement(2326..3111)
                     /gene="allE"
                     /locus_tag="POB34_002485"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HDI5919416.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGYAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(3122..4357)
                     /gene="allC"
                     /locus_tag="POB34_002486"
     CDS             complement(3122..4357)
                     /gene="allC"
                     /locus_tag="POB34_002486"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HDI5919417.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMIRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLNGTEYPQEVILSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(4379..5428)
                     /gene="allD"
                     /locus_tag="POB34_002487"
     CDS             complement(4379..5428)
                     /gene="allD"
                     /locus_tag="POB34_002487"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HDI5919418.1"
                     /translation="MKINRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGFIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            5745..7412
                     /locus_tag="POB34_002488"
     CDS             5745..7412
                     /locus_tag="POB34_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDI5919419.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQSL
                     TQVRRWDSACQKLSDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSQDEAARLACLLSRVTARRNAITPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIRPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            7422..8681
                     /locus_tag="POB34_002489"
     CDS             7422..8681
                     /locus_tag="POB34_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDI5919420.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            8692..9507
                     /locus_tag="POB34_002490"
                     /pseudo
     CDS             8692..9507
                     /locus_tag="POB34_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            9504..10397
                     /locus_tag="POB34_002491"
     CDS             9504..10397
                     /locus_tag="POB34_002491"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDI5919421.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVDPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQKALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(10534..11601)
                     /gene="purK"
                     /locus_tag="POB34_002492"
     CDS             complement(10534..11601)
                     /gene="purK"
                     /locus_tag="POB34_002492"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDI5919422.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAE
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLSATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(11598..12107)
                     /gene="purE"
                     /locus_tag="POB34_002493"
     CDS             complement(11598..12107)
                     /gene="purE"
                     /locus_tag="POB34_002493"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDI5919423.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(12225..12947)
                     /gene="lpxH"
                     /locus_tag="POB34_002494"
     CDS             complement(12225..12947)
                     /gene="lpxH"
                     /locus_tag="POB34_002494"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HDI5919424.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQGLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(12950..13444)
                     /gene="ppiB"
                     /locus_tag="POB34_002495"
     CDS             complement(12950..13444)
                     /gene="ppiB"
                     /locus_tag="POB34_002495"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HDI5919425.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            13618..15003
                     /gene="cysS"
                     /locus_tag="POB34_002496"
     CDS             13618..15003
                     /gene="cysS"
                     /locus_tag="POB34_002496"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDI5919426.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(15039..15560)
                     /locus_tag="POB34_002497"
     CDS             complement(15039..15560)
                     /locus_tag="POB34_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDI5919427.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FNFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(15668..15880)
                     /gene="ybcJ"
                     /locus_tag="POB34_002498"
     CDS             complement(15668..15880)
                     /gene="ybcJ"
                     /locus_tag="POB34_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HDI5919428.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(15882..16748)
                     /gene="folD"
                     /locus_tag="POB34_002499"
     CDS             complement(15882..16748)
                     /gene="folD"
                     /locus_tag="POB34_002499"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDI5919429.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            17219..17761
                     /gene="fimA"
                     /locus_tag="POB34_002500"
     CDS             17219..17761
                     /gene="fimA"
                     /locus_tag="POB34_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDI5919430.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            17981..18673
                     /gene="fimC"
                     /locus_tag="POB34_002501"
     CDS             17981..18673
                     /gene="fimC"
                     /locus_tag="POB34_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDI5919431.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            18704..21312
                     /locus_tag="POB34_002502"
                     /pseudo
     CDS             18704..21312
                     /locus_tag="POB34_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            21325..>22007
                     /gene="fimH"
                     /locus_tag="POB34_002503"
     CDS             21325..>22007
                     /gene="fimH"
                     /locus_tag="POB34_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HDI5919432.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYILMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRP"
BASE COUNT         5253 a         5449 c         5865 g         5440 t
ORIGIN      
        1 tcgcctggca cggttattta cgccgtcggt gatggtcttg tttatgttga tgctgggcgc
       61 gcagctgacc actatctttt tcaaaggtat gctcgggctg ccgtttggca tagccgaccc
      121 aaattttaaa attcagttac cgccgttcgc gctctcggtg gcggtgatgt gcctggtact
      181 ggggatgatt atcttcctgc cgcaacgttt tgcccgttat ggcctgctgg tcggcaccat
      241 aaccggctgg ttgttgtggt acttttgctt tccttcttcg cactcgctct ccggtgagtt
      301 gcactggcag tggttcccgc tcggcagtgg aggtgctttg tctccgggaa ttattctgac
      361 ggcggtgatt acaggtctgg tgaatatcag caatacctac ggtgcgattc ggggcacgga
      421 tgttttttat ccgcagcagg gcgcgggaaa tacgcgttat cgtcgcagct ttgtggcgac
      481 cggatttatg acgctgataa ccgtaccgct ggcggtaatt ccattttcac cgtttgtttc
      541 atccattggt ttattaaccc agacaggcga ttacacgcgg cgttcgttta tttatggcag
      601 cgttatttgc ctgctggtgg cgctggttcc ggcactcaca cgactgtttt gcagtatccc
      661 tttacccgtg agtagcgcgg tcatgctggt ttcttatctg cctttactct tttccgcgct
      721 ggtgtttagc cagcaaataa cctttaccgc tcgcaatatt tatcgactcg cattgccgtt
      781 atttgtcggc atatttttaa tggcattacc gcctgtgtat ctgcaagacc ttccattaac
      841 gcttcgtcct ctgctcagta acggcttatt ggtcgggatt ttactggctg ttcttatgga
      901 taaccttatt ccgtgggaac gtatcgaata atttgttgaa aaaggattga taatgaagat
      961 tgtcattgcg ccagactctt ttaaagagag cttaagtgca gaaaaatgtt gtcaggcaat
     1021 taaagccggg ttttcgaccc tctttcccga tgcgaactat atctgtttgc cgatagcgga
     1081 tggcggcgaa ggaacggtgg atgcgatggt cgccgcgacg ggcggcaaca tcgtgacgct
     1141 tgaagtctgc gggccgatgg gcgaaaaagt gaatgctttt tatggcctta ccggcgacgg
     1201 gaaaacggcg gtgattgaga tggcggcagc aagtggcctg atgctggtcg cgcctgaaaa
     1261 gcgtaatccg ttgctggcct ccagttttgg tacgggggag ttaattcgtc atgcgctgga
     1321 taacggcatt cgccatatta ttctcggcat tggcggcagt gcgacggtcg acggcggtat
     1381 gggcatggcg caggcgcttg gtgtgcgttt ccttgatgcc gacggccagg tgctggcggc
     1441 taacggcggc aatttagcgc gcgtggcaag cattgagatg gaggaatgcg atccgcgtct
     1501 ggcgaacagc catattgaag tggcatgtga cgttgataac ccgctggtag gggcacgcgg
     1561 cgcggcggcg gtatttggcc cgcaaaaagg ggcaacgccg gagatggtcg aagagctgga
     1621 acaggggctg caaaattacg cccgtgtttt acaacagcaa actgaaatta atgtctgcca
     1681 gatggcgggc ggcggcgctg cgggcggtat gggtattgcg gcggcggtat ttctcaatgc
     1741 ggatattaaa ccgggcattg aaattgtgtt gaatgcagtc aatcttgcgc aggcagtgca
     1801 gggcgcagca ctggtgatca ccggggaagg gcgcatcgac tcgcaaacgg caggcggtaa
     1861 agcgccgctg ggtgtggcgt cggtggcgaa acagtttaat gtaccggtga ttggaattgc
     1921 tggcgtattg ggtgatggcg tggaagtggt acaccagtac ggcattgacg cggtattcag
     1981 cattttgcca cgtctggcac ctttagccga agtgctcgcc agcggtgaaa ccaatctctt
     2041 caacagcgcg cgaaatattg cctgcgccat taaaataggt cagggaatta aaaattgact
     2101 cttgccttta aagcggatgc tatttatttc gtataagagc gcagtgctca tgccggatgc
     2161 ggcatgagta ctatatcctt cctgaaaatc gcgcaaattc tatatattgc agagatcatg
     2221 taggcctgat aagcgaagcg catcaggcaa tgttacaaaa aaagccacgg tataaaccgt
     2281 ggcaaaatcc aacatagcta aaaataatca ggcgagtggt atgacttaaa tctctacgtc
     2341 gcggttgcaa tctttcgagt aaatatagct gaacgcttca ccacgcccta caccataacc
     2401 agcctgtaaa gaataagcgc ccataaagat gtaatcgcct tttttcaccg ggatccagtt
     2461 attgtcgagg ttataaaccc cctgaccgga aagaatatag gcaccgtgtt cctgaacgtg
     2521 tgtttcgata taaccgtggc tggcacctgg tgcaaaagag aggatatgca tgttcatatc
     2581 aaaacctaac tctttgggca gaaaatccag cagaataaca tcgtccatac cttcatagtg
     2641 aatgcgttcc agttcgctgg cattgccaga aaccagccac ggtgcatagc cttctaccgg
     2701 aacatagcgg cgcttatata aaaagatttg gctgtcttcg gcctgggcgt taacaaacgt
     2761 cattaaggag cctggcgggc aataaagata gccaccttcg cttaaggcaa atgttttgcc
     2821 ttcggctttg gcagtgatat ttccagagat cacatacagg aacgtttcaa tgccttcgcc
     2881 accgaagccc tgttggttgc caccgttttg atgcagtgtg accagataat caacaaaaga
     2941 ggcacccagc tttggcgtgg agaggattgt cgcgtcacaa ttttcaaagc ccggaataat
     3001 attttttacc agaccgtctg gtgttaacag tgcgaaatta ccgtgtttca caatcgcacg
     3061 gttagccagt aaatcatcgc ggtaaccggt gacgttattt aaatatccca tttataactc
     3121 cttatttctg ccaggcaagt tgataaagca tgagtgccaa cgttttgacc ccttcggcaa
     3181 ggtcggtaat attggtgcgt tccgccgggt tatggctgat cccgttgatg ctggggataa
     3241 aaatcatgca ggttggtacg cgaggcgcga aaatttgcgc gtcgtgcccg gcaccactgt
     3301 gcatcacccg gtaattcagt ttttctcttt cacacaattc tgtcagggtg gcgaccagct
     3361 ccttattcat cggcacgggt tcttcgtcca tccataaatc gatatcaata ccaatgtcca
     3421 tttcatcgca aatcgcccgc atgtcgtttt ctaactgttg ggtgaaatca cgcaggacgg
     3481 cggcgtcggt atgacgacaa tcaatggtga acgtggtttt acccggcacc acattcaccg
     3541 tattcgggcg cggctccacc ttaccaaagg tcagaaccag cggatcgccc atctttttcg
     3601 ctttttcgat cgactgatgg caaatgcgac tgaaagcgta aaccgtatca cgacgataac
     3661 ccatcggcgt ggtgcctgca tggtttgatt cgccgttcag cgtcaccgta tagcgacgct
     3721 gcccgacaat tgcattcacc acgccaattg attgaccatt actttccagc acacagccct
     3781 gttcaatatg cagttcaaca aaagctttaa tatcctgacg cggagttagt ggggcgttcg
     3841 gcagagtaaa tccgcaagcc ttcatcgcat cgacaaaact atttcctttg gcatcacaga
     3901 tattccgcac gtcgtcagga ttcgccagcc caaagatatt tttactgccc cagaagacat
     3961 acgggaagcg gctgccttct tcttctgcca tcgccaccac ttcgaccgta cgtagcggcg
     4021 cgccgtattg cgttttcagc cagtcaattg ccagccacgc cgccagcgcg ccgaattgcc
     4081 cgtcgaggtt accgccgtta accacggtat cgatatgcga accgctcaga atcacttcct
     4141 gtggatattc ggtaccattc aggcgaccgt ataaattccc tacttcatcg aaacgtgttt
     4201 ccagcccact tgctgccatt ctttttttaa attgctgctg ggtttccagc cattccggcg
     4261 aataaagtaa acggatcatc ccacccgttg ggtcagcgcc aaaagaggaa agccagggca
     4321 gcgtttcttc tatggcttga cgaaaatgtg taatcataag aaagtcctgt ctcaataatt
     4381 attgcgcaaa gggatttttc gtttcgtatg acgtgttata aagcgcgtcg gaaattaaat
     4441 actggtaaat atcatcaaca atttcgatgc cttcgacggc ggctttgcgt tgtttaatat
     4501 cctgatcctg tccgggataa taaacttggt taaaaccggg cgcgggggta atggcattta
     4561 attcgcgcat ggtctggcta agatgttgac ggaataattc gctggaggag aaaaagttcg
     4621 gattaataac tatatgtaat tgccccaaat tacgccctgc gtgtaaatcg tcatacatcg
     4681 aactaacctg tcgcccgaac ggtaagccga gtaagacgcc tgagaggacg tcaatcatca
     4741 tcatcaggcc ataccctttt ggcccggcgg cggggagcag agcatgtacc gcgaacggat
     4801 cggttgttgg tgcaccgttt ttatcgaccg cccaggtatc cgggatagac atattacgcg
     4861 agcgggcgtc gagcactttt ccccatgcct gtacggtagt cgccatatca aaggtaagga
     4921 tctcgtcgcc ttctcccggc gcggcaaagg ccagggggtt agtaccgtag taaatttccg
     4981 cgccgccaaa cggcaccacc attggatcgg actggcacat cgaaatgcca atgaatccgg
     5041 cgcgggctgc ctgctgcaca aaataagaga ttgcgccgct gtgacccatc cggctgatac
     5101 cgaccaccgc aacgccattt tgctgggcgg ttttgatggc atgttccata cccattttcg
     5161 ccgcgacctg tccggcggca ttgtcggcat gtaaaattgc cgagcacggc ccggtttcct
     5221 caagacgaaa ctccggttcg cggttggtgc cgccttttga aatgcgttcc gcgtagtatt
     5281 ccacgcgcac cgcgccatga gagtggatcc ctctggcatc ggcgtaaacc aatacttcag
     5341 ccacggttgc agcgtgctca cgttttaacc cagcctggca gagtttattc tcaattagct
     5401 ggtggagtgt ttcccgattg attttcatct gtcttccttt ttaacgacgg tgtgaagcat
     5461 gactgcaatt aacatacagg gaaaatatct ggattatgtg atccagacag gcaaaaaaat
     5521 atagttagaa tttatttgat aatccgctca cttttaacct gatttttaaa acaaccacgc
     5581 ttattaaaaa ataatgcgta atagcctggc ggttatttga attcttttgt taataattcc
     5641 tgtgtgatat tcatcacctt atttactcgt tgtcatcgat accgtaatcg ccacattaac
     5701 actgctcgtg caattgccat gggtgcaatt tttaaggagt tgttatgatc cacgccttta
     5761 ttaaaaaagg gtgttttcag gattcggtca gtttaatgat tatttcacga aaactcagcg
     5821 aatcagaaaa tgttgatgat gtttccgtaa tgatgggtac gcccgccaat aaagcgttat
     5881 tagataccac aggtttctgg catgacgatt ttaataacgc cacgccgaac gatatttgcg
     5941 tggcaatacg tagcgaagcg gcggatgcgg ggatcgcgca ggcgattatg cagcagcttg
     6001 aagaggcgct aaaacaactg gcgcaggggt caggcagcag ccagtccttg acgcaggtgc
     6061 gtcgctggga cagtgcctgt cagaaattat ccgatgccaa tctggcgctg atttcagtgg
     6121 ctggcgagta tgcggcggag ctggcgaatc aggcgctgga tcgcaacctc aacgtgatga
     6181 tgttctccga taacgtcacg ctggaagatg aaatccaact taaaacccgc gcgcgagaaa
     6241 aaggcttgct ggtgatgggg ccggactgcg gtacgtcgat gattgccgcc acgccgctgg
     6301 cttttgctaa cgtgatgccg gaaggcaaca ttggcgtcat tggcgcttcc ggtaccggga
     6361 ttcaggagct gtgctcacag attgcgctgg caggggaggg aattactcac gcgattggcc
     6421 ttggcgggcg cgacctcagc cgtgaagtgg gcggcatcag tgcgctgacg gcgctggaaa
     6481 tgctcagtgc agacgagaaa agcgaagtgc tggcatttgt ttcaaaaccg cctgccgaag
     6541 ctgtgcgtct gaaaattgtt aatgccatga aagcaaccgg caaaccgacg gtggcgctgt
     6601 ttttaggtta taccccggcg gtggcccgcg acgagaatgt ctggtttgcc tcctcgcagg
     6661 atgaggccgc acgcctggct tgcctgcttt cacgcgttac ggcgcgacgt aacgcgataa
     6721 cgcctgccag cagcggattt atttgcggtt tgtataccgg cggtacgctg gctgccgaag
     6781 cggcgggatt acttgccggg caccttggcg tggaagccga cgatacccat catcacggca
     6841 tgatgctcga cgccgatggt caccagatta ttgacctcgg cgatgatttc tacaccgtcg
     6901 ggcgtcccca tccgatgatc gacccagcct tacgcaatca gttaattgcc gatctcggcg
     6961 ctaaaccgca agtgcgcgta ttgctgcttg atgtcgtgat tggcttcggt gcgaccgccg
     7021 atcctgccgc ctcgctggta agcgcctggc aaaaagcatg tgccgcgcgt tcagacaatc
     7081 aaccactgta tgccattgcc acggtgacag gcactgaacg tgacccgcaa tgccgctcgc
     7141 agcaaatcgc cacgctggaa gatgcaggga tcgcggtagt gagttcgctg ccggaagcca
     7201 ccttgctggc ggcggcactg attcgtccgc tttcgcctgc cactcagcaa cacacaccgt
     7261 cattactgga aaacgtcgcc gtgattaaca tcggattacg cagctttgcg ctggagctac
     7321 aaagcgccag caaaccggtt gtgcattacc aatggtcgcc agtcgccggc ggcaataaaa
     7381 aactggctcg tttattagaa cgtttgcaat aaggggttcc catgtttaca tcagtggcgc
     7441 aagccaatgc tgcggttatc gaacaaattc gtcgtgctcg tccacactgg ctggatgtgc
     7501 aaccggcttc ttcacttatc agcgaactaa acgagggcaa aacactgctt cacgccgggc
     7561 cgccaatgcg ctggcaggag atgaccggac ccatgaaagg ggcgtgcgtg ggcgcatgtc
     7621 tgttcgaagg ctgggcgaaa gatgaagcgc aggcgctggc aatactggag cagggggaag
     7681 tgaacttcat tccttgtcac catgtgaatg ccgtcggtcc aatgggcggt attacttctg
     7741 ccagtatgcc gatgctggtg gtcgagaacg tgaccgacgg caaccgggcg tactgcaacc
     7801 tcaacgaagg tatcggcaaa gtgatgcgtt ttggcgctta cggcgaagat gtcctgactc
     7861 gccatcgctg gatgcgcgat gtgttaatgc cagtattaag cgcggcgctg gggcgcatgg
     7921 agcgcggtat cgatctcacg gcgatgatgg cgcagggcat tacgatgggc gatgagttcc
     7981 atcaacgcaa tattgcttcc tctgcactgt taatgcgtgc gctggcccca caaattgctc
     8041 gcctcgatca tgataaacag cacatcgccg aagtgatgga tttcctcagc gtgaccgatc
     8101 agttcttcct caacctcgcg atggcttact gcaaggcggc gatggatgct ggcgcgatga
     8161 tccgcgcagg cagcatcgtc acggcaatga cccgcaacgg caatatgttc gggattcggg
     8221 taagcgggct gggcgaacgc tggtttactg cgcctgtaaa cactccgcaa ggtctgtttt
     8281 tcaccggctt ctcgcaggag caggcgaacc cggatatggg cgatagcgcg attaccgaaa
     8341 cctttggtat cggaggtgcg gcaatgatcg cagcgcctgg cgtaacgcgc tttgtcggtg
     8401 cgggtggcat ggaagcggcc agagcggtat ctgaagagat ggcggaaatt taccttgagc
     8461 gcaatatgca gttgcagatc ccaggctggg attttcaggg cgcgtgcctg ggactggaca
     8521 ttcgtcgcgt ggtagaaacc ggcattacgc cactcatcaa taccggtatc gcccataaag
     8581 aggcggggat cgggcagatt ggcgcaggca ctgtgcgggc accgctggcg tgttttgaac
     8641 aggcgctgga agcactggct gaaagcatgg ggattggttg aggaacgcgc aatgacgatc
     8701 atccatcctc tgcttgccag ccgtagtgca ccgaattatc gccagtcctg gcggttagcg
     8761 ggagtgtggc gacgggcgat taacctgatg acggaaagcg gcgaactgtt aacgctgcat
     8821 cgtcagggta gtggtttcgg ccccggagga tgggtgcttc gccgcgcgca attcgatgcg
     8881 ttatgcggtg gattatgcgg caatgaacga ccacaggttg tggctcaagg gattcgcctc
     8941 gggcgtttca cggttaaata gccacagcgt tattgtttgc tgcgtattac gccgccttca
     9001 catcctcaac cgcttgcagc tgcatggatg caacgcgcgg aggaaaccgg acttttcgga
     9061 ccactggcga tggcggcaag cgatccgctg accgctgaat tacgccagtt tcgtcactgt
     9121 tttcaggccg cgctcaatgg cgttaagacc gactggcggc actggctggg taaaggcccc
     9181 ggattaacgc caagtcatga tgacacgttg agcggaatgc tgctggcggc ctggtattat
     9241 ggcgctttag atgcgcgctc cggtcgccag ttttttgcct gctccgacaa tcttcaactc
     9301 gttaccacag cggtgagcgt cagttattta cgctacgccg cgcaaggata tttcgcctcg
     9361 ccactcctgc actttgttca tgctctgagt tgcccgaaac gtaccgctgt tgcgattgat
     9421 tcgctgctgg cgctggggca cacgtcaggg gcagatacgc tgctggggtt ctggcttggt
     9481 caacaattat tacaaggaac accatgaaaa cactggttgt agctcttggg ggcaacgcct
     9541 tactccagcg cggtgaggcg ctgacggcag aaaatcaata tcgcaatatc gccagtgctg
     9601 tacccgcgct ggcgcgcctg gcccgttctt atcggctggc gattgttcac ggcaatgggc
     9661 cgcaggtggg gctgctggca ttgcagaatc tggcgtggaa agaggtagat ccgtatccgc
     9721 tggatgtgct ggtggcggaa agccagggga tgattggcta tatgctggcg cagagtttga
     9781 gcgcacagcc gcagatgccg cctgtcacga cggtgctgac gcgcattgag gtttcgcctg
     9841 atgatccggc gtttttgcag ccagagaaat ttattggtcc ggtttatcag ccagaagaac
     9901 aaaaggcact ggaagcggct tacggctggc agatgaaacg tgatggtaaa tatttgcgcc
     9961 gggtggtggc gtctccgcaa ccgcgtaaaa ttctcgacag cgaagccatc gagttgttgc
    10021 tcaaagaggg gcatgtggtg atttgcagtg gcggcggcgg tgtgcctgtg acggaagatg
    10081 gggcagggag tgaagcggtg attgataaag atctcgccgc tgcgttgctc gccgagcaga
    10141 ttaatgccga tggactggtg atcctcaccg atgctgacgc ggtttatgaa aactggggaa
    10201 cgccgcagca acgcgccatt cgccatgcca caccggatga gttagcgcca tttgccaaag
    10261 ccgatggttc gatggggccg aaggtaacgg cggtaagtgg ttatgtcaga agccgtggta
    10321 aacccgcgtg gattggggcg ttatcgcgaa ttgaagagac gctggcgggc gaagcgggga
    10381 cctgtatttc gctgtagtga tgagagcatc cggcaatcgg tgcaccatgc ctgatgcgac
    10441 gcttgacgcg tcttatcagg cctacaatgg gtaccggagc ggtaagccgg ataaggcgtt
    10501 tacgccgcat ccggcaatca aatgagcacc aggttaactg aacttactct gtgcccacat
    10561 cacgccgctg gcatattccg gcggcagcag cgggattaag gcttccagcg tcgcggacag
    10621 acgcgatgtg tcgctgtcgg tcaaattcag atgccccact ttacgccccg gacggacttc
    10681 tttgtcgtac cagtgcagat gcaccagcgg caatttcagc cagtcataat tcacatcgct
    10741 accaatcaga ttgatcatca ccgacggact attcactact ggttgcggta acggcagatc
    10801 ggtaatcgcc cgcagatgca gctcaaactg gctgatgctg gcaccgtttt gtgtccagtg
    10861 accgctgtta tgcacacgcg gagccagttc gttgatcagc agaccttgcg gggtgacaaa
    10921 acactccatc gccatcacgc ccacatagcc cagctcctgc ataatcgccg acagcatctc
    10981 ttcggcttgc gcctgctgct gtgcgttggc ctgcggaaaa gcgacgctgg tgcgcaaaat
    11041 accgtcctga tgcaggttat gcgtcagcgg ataaaacacg gtgctgccat caaagccgcg
    11101 cgcgccaacc agcgacactt caccagagaa gttaatgccc tgctcgacaa tacattcgcc
    11161 gtaacactct gccggtaact gttcggtttc atttgcgcgt aaacgccatt gaccgcgacc
    11221 gtcataacca ccagtgcgac gcttaacaat cgccagctca cctaaacgat caaacaccgc
    11281 aggccactcg ctgcgttcgg caagtaactg ccacggtgca gtcggcaggt ggagcttatc
    11341 gaaaagctgc ttctgagtca gacggtcagc aataatcggg aacacatcgc ggttcacaaa
    11401 ggccggatgg cgcgccagct cgcgggttaa tgcggtttcc ggccagcgtt ctatctcagc
    11461 ggtaatcacg ctttgttgaa aaggcaccgc cgccggttca gcgtccagcc cgactggcca
    11521 gacagcaatg cctaacggtt cgcctgcctg acgcagcata cggcctaact gcccgttacc
    11581 gaggacgcaa acctgtttca tgccgcacct cgcgggtccg ggttttccag cacttcgtcg
    11641 gtctgggctt tgcgccagtc attcagacgc tggtgcagtt ctttatcatg agtcgcaaga
    11701 atttgtgctg ccagtaacgc cgcgtttgcc gcgccagctt taccaatcgc cagcgtaccc
    11761 accggaatgc cgcgcggcat ttgtacgatg gagtagaggc tatcgacacc gctcagtgcg
    11821 gcgctctgta ctggcacgcc cagcaccggc accagcgttt tggcggcaat catgcctggc
    11881 agatgcgctg cgccgcctgc gcccgcaata atcacctgat aaccgttctc ttcggcgctt
    11941 tcggcgaagc tgaacagttt atcgggggtg cggtgagcag aaaccacttc aacgtggtgc
    12001 gggacattca ggatttcgaa gatttcggcg gcgaactgca tggtagccca gtcgcttttg
    12061 gaccccatca cgatggcgac acgcgccgga ttattgcggg aagacatgcg tcttaaaact
    12121 cctgtggtgc acaactctcg gctttagagg gcacagagaa tagcacggaa agagagcaag
    12181 gaaaacggtt gcgtggctgt gaaatcagca aagttgcggg ttttttaaaa cggaaaatga
    12241 attagctcaa cgtcatccgc cgtgactttc accatcgaac cttccgtatg ccaggcaccc
    12301 agtaccacgc gaaaagcagg ttgctgattg gcgataagtt catgcaccgc cgggcgatgg
    12361 gtatgcccgt ggatcagccc ttgcacctga tgtttttcca tcgcactgac taccgcgttt
    12421 tggttaacgt ccatgatcgc cagcgattta ctgctattgg cttctttact gttcgcgcgc
    12481 attcgcgcgg caatgcgttt gcgcacaaac aacggcagag cgaggaatag catttgcagc
    12541 cagggtttgt ggactttggc gcgaaaagcc tgataacccg cgtcatcggt gcacagcgtg
    12601 tcgccatgca taatcaacac ccggcgacca taaagttcga gcaccttttc ttccggcaat
    12661 aatgtcatgc cgctttcacg ggcaaagcgt ttgccgagca gaaaatcacg gttgccatga
    12721 atgaaataac agggaacgcc ggaatcggac accgctttga tcgctgccgc catctggcga
    12781 tggagtgggt tgggatcgtc gtcgccaatc catgcttcaa acagatcgcc aagaatatac
    12841 agcgcgtcgg ccttgcgggc ttcccccgct aaaaaacgca gaaaaccggc ggtgatcgcc
    12901 ggttcttcca cgcagagatg aagatctgca ataaagagtg tcgccacgat tactcgctaa
    12961 cggtcacgct ttcaatgata acgtcttctt ttggcacgtc ctggtgcata ccgctacgac
    13021 cggttgcaac acctttgatt ttgtctacca cgtccatgcc gtcaaccact tcagcaaaca
    13081 cgcagtagcc ccaaccttgc aggctttcgc cagagaagtt caggaagtcg ttatcaacca
    13141 cgttgatgaa gaactgtgca gttgcagagt gcggagcctg agtacgtgcc attgccagcg
    13201 taccacgggt atttttcagg ccgttgttgg cttcgttttt gatcggttct ttggtggctt
    13261 tttgtttcat gcccggttca aaaccgccgc cctgaatcat aaagccgttg ataacacggt
    13321 ggaaaatggt gttgttgtaa aaaccttcgc ggcagtagtc caggaagttt ttaactgttt
    13381 caggtgcttt atcgtcaaaa gttttgatga caatatcgcc gtgattggtg tggaaagtaa
    13441 ccatttttgc atcctgttcc gtttgattgg tgcttcaacc cagttcgggt catatatagg
    13501 gtggtgttat agcataaccg cacgatcgga tcatcacgca atgtatgctg attcgcgcgg
    13561 gaaatatggg tattatacgc aactcaatta cccacacatg tctaaacgga atcttcgatg
    13621 ctaaaaatct tcaatactct gacacgccaa aaagaggaat ttaagcctat tcacgccggg
    13681 gaagtcggca tgtacgtgtg tggaatcacc gtttacgatc tctgtcatat cggtcacggg
    13741 cgtacctttg ttgcttttga cgtggttgcg cgctatctgc gtttcctcgg ctataagctg
    13801 aagtatgtgc gcaacattac cgatatcgac gacaaaatca tcaaacgcgc caatgaaaat
    13861 ggcgaaagct ttgtggcgct ggtggatcgc atgatcgccg aaatgcacaa agattttgat
    13921 gcgttgaaca ttctgcgccc ggatatggag ccgcgcgcga cgcaccatat cgcagaaatt
    13981 attgaactca ctgaacaact gatcgccaaa ggtcacgctt atgtggcgga caacggcgac
    14041 gtgatgttcg acgtcccgac cgatccaact tatggcgtgc tgtcgcgtca ggatctcgac
    14101 cagctgcagg caggcgcgcg cgttgacgtg gtcgacgaca aacgcaaccc aatggacttc
    14161 gttctgtgga agatgtcgaa agagggcgaa ccgagctggc cgtctccgtg gggcgcgggt
    14221 cgtcctggct ggcacattga atgttcggca atgaactgca agcagctggg taaccacttt
    14281 gatatccacg gcggcggttc agacctgatg ttcccgcacc acgaaaacga aatcgcgcag
    14341 tccacctgtg cccatgatgg tcagtatgtg aactactgga tgcactcggg gatggtgatg
    14401 gttgaccgcg agaagatgtc caaatcgctg ggtaacttct ttaccgtgcg cgatgtgctg
    14461 aaatactacg acgcggaaac cgtgcgttac ttcctgatgt cgggccacta tcgcagccag
    14521 ctgaactata gcgaagagaa cctgaagcag gcgcgtgcgg cgctggagcg tctctacact
    14581 gcgctgcgcg gcacagataa aaccgttgcg cctgccggtg gcgaagcgtt tgaagcgcgc
    14641 tttattgaag cgatggacga cgatttcaac accccggaag cctattccgt gctgtttgat
    14701 atggcgcgtg aagtaaaccg tctgaaagca gaagatatgg cagcggcgaa tgcaatggca
    14761 tctcacctgc gtaaactttc cgccgtattg ggcctgctgg agcaagaacc ggaagcgttc
    14821 ctgcaaagcg gcgcgcaggc agacgacagc gaagtggctg agattgaagc gttaattcaa
    14881 cagcgtctgg atgcccgtaa agcgaaagac tgggcggcgg cagatgcggc gcgtgaccgt
    14941 cttaatgaga tggggatcgt gctggaagat ggcccgcaag ggaccacctg gcgtcgtaag
    15001 taattgcgct attgccggat gcgagttttc gcatccggtt atcgtctgcg ccaccacaac
    15061 attcccatca gtagcatccc cggcaaccac acccacatca attcagaaat aatcacctga
    15121 tgcccgtacg ctgtggtgta acgagacaat gcaaacggcg cgactttaat cacctgccag
    15181 ggagcgaaaa agcgttcatc tgaccacggc cacagccagc caaccccttt accgccagtg
    15241 gttaccgaat ccagcaagct gtgcgatagc aacgagacgg ttaaaaacag ccagcagcga
    15301 atcagcccag ccctgaacca tcgacgtcca atcagcacac ataacagcgg gacaacaaac
    15361 gcaaacacca gcgaatgggt aaacccgcga tgaccaaaaa cattgccgta agcaacgcca
    15421 aaattaaacg acaagacgtc ggcatcgggc agcatcgcca ggatgattcc ggcaaataac
    15481 aggcgcggag ggatgacttt cgaacccaac cctaaaccaa tgcatagggg aacggcggcg
    15541 tgcgtaataa cggttggcat gatggtcgct tcggcaaaat gtcgatgcta tcagcatgga
    15601 tgaacggggc gtagagggca aaagtctgaa aagagaaccg gcctgttgat acaggccggg
    15661 aaagggatca ggcaacaacc tgtacgctgt gacctgcaaa actcactgtc tgaccggcga
    15721 cgattttgca gcgtttgcgc gtttcaaccg caccgtcgac tttcacctgg ccttcggcaa
    15781 tcgcgatttt cgcctgcgcg ccgctttcgc tccagccttc cagtttcagc aagtcgcaca
    15841 gctcaacgtg cggatgttta cctaaagaaa atgtcgccat gttactcatc ctgtggatca
    15901 tgatattcaa cgcacgcctg tagcgtgttt tcaatcagcg tggcaaccgt catcgggcca
    15961 acgccgccgg gaacaggcgt aatgtatgag gcgcgtttag ccgcgtcttc aaacacgacg
    16021 tcgcccacaa ctttgccatt ttccagacgg ttgatgccga catcaatcac aattgcgcct
    16081 tctttgatcc agtcaccggg aataaagcct ggcttgccaa cggcaacgat caatagatcg
    16141 gcattttcta cgtgatgacg cagattttta gtgaagcggt gagtcactgt agtggtgcaa
    16201 cctgccagca gcagttccat gctcatcggg cggccaacga tattcgatgc gccaatcacc
    16261 acggcgttga ggccgaaggt atcaatgttg taacgctcaa gcagcgtgac gataccgcgc
    16321 ggggtgcagg gacgcagacg cggcgcgcgc tggcacagac gaccgacgtt gtaaggatgg
    16381 aaaccgtcca cgtctttgtc cggatgaata cgttccagca ctttgacgtt atcaataccc
    16441 gccggtaacg gcagttgaac cagaatgcca tcgatggtgt tgtcggcatt cagcgtatcg
    16501 ataagctcca gcagctccgc ttcgctggtg gtttccggga ggtcataaga gcgggagacg
    16561 aacccgactt cttcacaagc cttgcgtttg cttgcgacat aaatttgcga tgcagggtta
    16621 ctacccacca gcacaacggc cagtcctggt gcccgcagtc cggctgcaat acgcgcctga
    16681 actttttgag caacttcaga gcgcacctgc tgcgcaatcg ttttaccgtc aataatcttt
    16741 gctgccatca gagagaggat tccatctgtt acgtaggtcg aaggggatgc gcctattttg
    16801 tcagaagcgg ggcgcgctgt caggtttcgt ttcagattta tcgcgtgaag cgacctcttg
    16861 cgaaggtgag gcgcaccgtc gctgagactg aaagcttcat ttttcgtcca tgatggcgtt
    16921 gtaaatctgg aactgattta tttccttgtc taaggattaa gataatttaa gaaatacctg
    16981 acaatataaa aagaattttc agcctggtaa tttaccgctt caggtctata tttgtgttga
    17041 atatattatg cgcggaagta ttcatctaac ggggctctct attttttaga atagagtgca
    17101 tattttcaat taagacattc ttagaggata aaaaggaatt tactactatc agtgtcttaa
    17161 ataaagtaat cggttatata cggatgtgga gtcgataaat gagattgaag gaatatatat
    17221 gaaattaaga tttatttcgt ctgcgctggc tgccgcacta ttcgccgcta cgggtagtta
    17281 tgctgccgtt gtagatggcg gtacaattca ctttgaaggc gaactggtga atgctgcctg
    17341 ttcagtgaat actgactcgg cagaccaggt tgtcacactc ggtcaatatc gtaccgatat
    17401 tttcaatgct gttggtaata cctctgcatt aattccattc accattcagt tgaacgactg
    17461 cgatcctgtt gttgccgcta atgctgccgt tgcattttct ggtcaggctg atgcaatcaa
    17521 tgataattta ttggccattg catccagtac caatacaaca acagcaacgg gtgtcggtat
    17581 tgaaatactt gataatacat ccgcaattct caaacctgat gggaatagct tctcaaccaa
    17641 ccagaacttg atccccggga ccaacgttct tcatttttct gcacgttata aaggcaccgg
    17701 tacaagtgca tcagcagggc aagcaaatgc tgacgcgact tttattatga gatatgaata
    17761 atcaaaacca cgttgttttg aattatatat cacgtcttat aacaaagtaa tgtaccggtt
    17821 gtctgaagcg gtatggtggc aatgtaaatc gaaatcatgt tcactttgta tcatgccgct
    17881 ttattaaatg aaaagggaat gatgtgttgt aagaaaccaa agcaatcatt tctttatatt
    17941 ccttattttt gccgtcagga atacacaagg cgtattaact atgatgacta aaataaagtt
    18001 attgatgctc attatatttt atttaatcat ttcggccagc gcccatgctg ccggagggat
    18061 cgcattaggt gccacgcgta ttatttatcc cgctgatgct aaacagactg cggtatggat
    18121 tagaaatagc cataccaatg agcgctttct ggtcaattcg tggattgaaa acagcagcgg
    18181 tgtaaaagaa aagtcattca tcattacacc gccactgttt gttagtgaac ccaaaagcga
    18241 aaataccttg cgtattattt acaccggtcc accgctggcg gcagatcgtg agtctctgtt
    18301 ctggatgaat gttaagacga tcccttcggt agataaaaat gcattgaacg gcaggaatgt
    18361 tttgcaactg gcgattttat cgcgcatgaa attatttctc cgtccaattc aattacaaga
    18421 attacccgca gaagcgccgg acacactcaa gttttcgcga tccggtaact atatcaatgt
    18481 tcataatcca tcaccttttt atgtcaccct cgttaacctg caagtgggca gccaaaagtt
    18541 ggggaatgct atggcggcac ccagagttag ttcacaaatt cccttaccct caggagtgca
    18601 gggaaagctg aaatttcaga ccgttaatga ttatggttca gtaaccccgg tcagagaagt
    18661 gaacttaaac taaccgaatc atctgacaat atcagagcta attatgaaaa tacccactac
    18721 tacggatatt ccgcagaggt atacctggtg tctggccgga atttgttatt catctcttgc
    18781 cattttaccc tcctttttaa gctatgcgga aagttatttc aacccggcat ttttattaga
    18841 gaatggcaca tccgttgctg atttatcgcg ctttgagaga ggtaatcatc aacctgcgcg
    18901 cgtgtatcgg gtggatctct ggcgtaatga tgagttcatt ggttcacagg atatcgtatt
    18961 tgaatcgaca acagaaaata caggtgataa atcaggtggg ttaatgccct gttttaacca
    19021 ggtactcctt gaacgaattg gccttaatag cagtgcattt cccgagttag cccagcagca
    19081 aaacaataaa tgcatcaatt tactgaaagc tgtacctgat gccacaatta actttgattt
    19141 tgcagcgatg cgcctgaaca tcactattcc tcagatagcg ttgttgagta gcgctcacgg
    19201 ttacattccg cctgaagagt gggatgaagg tattcctgct ttactcctga attataattt
    19261 caccggtaac agaggtaatg gtaacgatag ctattttttt agtgagttca gcgggattaa
    19321 tattggcccg tggcgtttac gcaacaatgg ttcctggaac tattttcgcg gaaatggata
    19381 tcattcagaa cagtggaata atattggcac ctgggtacag cgtgccatta ttccgctgaa
    19441 aagtgaactg gtaatgggag acggcaatac aggaagtgat attttcgatg gtgttggatt
    19501 tcgtggtgta cggctttatt cttccgataa tatgtatcct gatagccagc aagggtttgc
    19561 cccaacggta cgtgggattg cccgtacggc ggcccagcta acgattcggc aaaatggttt
    19621 tattatctat caaagctatg tttcccccgg cgcttttgaa attacagatt tgcacccgac
    19681 atcttcaaat ggcgatctgg acgtcaccat cgacgagcgc gatggtaatc agcagaatta
    19741 cactatcccg tattcaacag tgccaatttt acaacgcgaa gggcgtttca aatttgacct
    19801 gacggcgggc gattttcgta gcggtaatag tcagcaatca tcgcctttct tttttcaggg
    19861 cacggcactc ggcggtttac cacaggaatt tactgcctac ggcgggacgc aattatctgc
    19921 caattacacc gcctttttat tagggctggg gcgcaacctc gggaactggg gggcagtgtc
    19981 gctggatgtg acccatgcgc gcagtcagtt agccgacgac agtcgtcatg agggggattc
    20041 cattcgcttc ctctatgcga aatcgatgaa caccttcggc accaattttc agttaatggg
    20101 ttaccgctat tcgacacaag gtttttatac ccttgatgat gttgcgtatc gtcgaatgga
    20161 ggggtacgaa tatgattacg attacgacgg tgagcatcgc gatgaaccga taatcgtgaa
    20221 ttaccacaat ttacgcttta gccgtaaaga ccgtttgcag ttaaatattt cacaatcact
    20281 taatgacttt ggctcgcttt atatctctgg tacccatcaa aaatactgga atacttcgga
    20341 ttcagatacc tggtatcagg tggggtatac cagcagctgg gttggcatca gttattcact
    20401 ctcattttcg tggaatgaat ctgtagggat ccccgataac gaacgtattg tcggacttaa
    20461 tgtttcagtg cctttcaatg ttctgaccaa acgtcgctac acccgggaaa atgcgctcga
    20521 ccgcgcttat gcctccttta acgccaaccg taacagcaac gggcaaaata gctggctggc
    20581 aggtgtaggt gggaccttac tggaaggcca caacctgagt tatcacgtaa gccagggtga
    20641 tacctcgaat aatgggtata cgggcagcgc cacggcaaac tggcaggccg cttacgctac
    20701 gctgggggtc gggtataact acgaccgcga tcaacatgac gttaactggc agctgtctgg
    20761 cggtgtggtc gggcatgaaa atggcataac gctgagccag ccttttgggg ataccaatgt
    20821 tttgattaaa gcgcctggcg caggcggtgt acgcattgaa aatcaaactg gcattttaac
    20881 cgactggcgc ggctatgcgg tgatgccgta tgccacggtt tatcggtata accgtatcgc
    20941 gcttgatacc aatacgatgg ggaattccat cgatgttgaa aaaaatatta gcagcgttgt
    21001 gccgacgcaa ggcgcgttgg ttcgtgccaa ttttgatacc cgcataggcg tgcgggcgct
    21061 cattaccgtt acccagggcg gaaaaccggt gccgtttgga tcactggtac gggaaaacag
    21121 tatcggaata accagtatgt gggtgatgac gggcaagttt atttaagtgg tgcgccattg
    21181 tctggtgaat tactggttca gtggggagac ggcgcgaact cacgctgcat tgcgcactat
    21241 gtattgccga agcaaagctt acagcaagcc gtcactgtta tttcggcagt ttgcacacat
    21301 cctggctcat aaaggaaatt atcaatgaag ataatctgca gattattatt ggcgatggca
    21361 tgtttgtgtc tggcaaacat atcctgggct actgtttgtg caaatagtac tggcgtagca
    21421 gaagatgaac actatgatct ctcaaatatc tttaatagca ccaataacca gccagggcag
    21481 attgttgttt taccggaaaa atccggctgg gtaggtgtct cagcaatttg tccacccggt
    21541 acgctggtga attatacata ccgtagttat gtcaccaact ttattgttca ggaaactatc
    21601 gataattata aatatatgca attaaatgat tatctattag gtgcgatgag tctggttgat
    21661 agtgtgatgg atattcagtt ccccccgcaa aattatattc tgatgggaac agatcctaac
    21721 gtttcgcaaa accttccatt cggggtgatg gattctcgtt taatatttcg tttaaaggtt
    21781 attcgtccct ttattaacat ggtggagatc cccagacagg tgatgtttac cgtgtatgtg
    21841 acatcaacgc cttacgatcc gttggttaca cctgtttata ccattagttt tggtggccgg
    21901 gttgaagtac cgcaaaactg cgaattaaat gccgggcaga ttgttgaatt tgattttggt
    21961 gatatcggcg catcgttatt tagtgcggca gggccgggta atcgacc
//
LOCUS       DAMZET010000065        21978 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.065, whole genome shotgun sequence.
ACCESSION   DAMZET010000065 DAMZET010000000
VERSION     DAMZET010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.065"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1798)
                     /locus_tag="POB34_002504"
     CDS             complement(<1..1798)
                     /locus_tag="POB34_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HDI5919433.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQT"
     gene            complement(1890..2390)
                     /locus_tag="POB34_002505"
     CDS             complement(1890..2390)
                     /locus_tag="POB34_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI5919434.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            complement(2420..3136)
                     /locus_tag="POB34_002506"
     CDS             complement(2420..3136)
                     /locus_tag="POB34_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDI5919435.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            3432..4208
                     /gene="exuR"
                     /locus_tag="POB34_002507"
     CDS             3432..4208
                     /gene="exuR"
                     /locus_tag="POB34_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HDI5919436.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            4553..5215
                     /locus_tag="POB34_002508"
     CDS             4553..5215
                     /locus_tag="POB34_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDI5919437.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            5219..5602
                     /gene="mzrA"
                     /locus_tag="POB34_002509"
     CDS             5219..5602
                     /gene="mzrA"
                     /locus_tag="POB34_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HDI5919438.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            5749..6117
                     /locus_tag="POB34_002510"
     CDS             5749..6117
                     /locus_tag="POB34_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HDI5919439.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            6155..6460
                     /locus_tag="POB34_002511"
     CDS             6155..6460
                     /locus_tag="POB34_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDI5919440.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            6463..6867
                     /locus_tag="POB34_002512"
     CDS             6463..6867
                     /locus_tag="POB34_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDI5919441.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            6857..7156
                     /locus_tag="POB34_002513"
     CDS             6857..7156
                     /locus_tag="POB34_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HDI5919442.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            7342..7734
                     /locus_tag="POB34_002514"
     CDS             7342..7734
                     /locus_tag="POB34_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDI5919443.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            7804..8790
                     /gene="yqjG"
                     /locus_tag="POB34_002515"
     CDS             7804..8790
                     /gene="yqjG"
                     /locus_tag="POB34_002515"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HDI5919444.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISNYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            9083..9448
                     /locus_tag="POB34_002516"
     CDS             9083..9448
                     /locus_tag="POB34_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDI5919445.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            9690..10046
                     /locus_tag="POB34_002517"
     CDS             9690..10046
                     /locus_tag="POB34_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDI5919446.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(10097..10993)
                     /gene="yhaJ"
                     /locus_tag="POB34_002518"
     CDS             complement(10097..10993)
                     /gene="yhaJ"
                     /locus_tag="POB34_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HDI5919447.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            11098..11799
                     /locus_tag="POB34_002519"
     CDS             11098..11799
                     /locus_tag="POB34_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HDI5919448.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            11822..11986
                     /locus_tag="POB34_002520"
     CDS             11822..11986
                     /locus_tag="POB34_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919449.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(12198..13508)
                     /locus_tag="POB34_002521"
     CDS             complement(12198..13508)
                     /locus_tag="POB34_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDI5919450.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(13536..14867)
                     /locus_tag="POB34_002522"
     CDS             complement(13536..14867)
                     /locus_tag="POB34_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDI5919451.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGYLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(15142..16506)
                     /gene="tdcG"
                     /locus_tag="POB34_002523"
     CDS             complement(15142..16506)
                     /gene="tdcG"
                     /locus_tag="POB34_002523"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDI5919452.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(16578..16967)
                     /locus_tag="POB34_002524"
     CDS             complement(16578..16967)
                     /locus_tag="POB34_002524"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HDI5919453.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(16981..19275)
                     /gene="tdcE"
                     /locus_tag="POB34_002525"
     CDS             complement(16981..19275)
                     /gene="tdcE"
                     /locus_tag="POB34_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HDI5919454.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(19309..20517)
                     /gene="tdcD"
                     /locus_tag="POB34_002526"
     CDS             complement(19309..20517)
                     /gene="tdcD"
                     /locus_tag="POB34_002526"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HDI5919455.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(20543..21874)
                     /gene="tdcC"
                     /locus_tag="POB34_002527"
     CDS             complement(20543..21874)
                     /gene="tdcC"
                     /locus_tag="POB34_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HDI5919456.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
BASE COUNT         5663 a         5654 c         5199 g         5462 t
ORIGIN      
        1 acgtttgatc gccatcctgt tcgctataac gataatccgt agacacattt gttgaatggc
       61 tgttgttatt gctatccatc gttggcgtgt cagataacga aaacgataag tacaagccgt
      121 tgtcgtcgtt atcgccaccg ttgtcattac gatggaaaac accgactgac gtactcaaaa
      181 tccagtcacg catcacaaaa gcgcgggaag cgctaagttg tacagtttca gagatacttt
      241 gcgctgttgt ctggcgaaga tagttatttt caaagctctg ctgtttatcc caacgcaaac
      301 tatcatcagt gttattttgt gtgcgggtat aacccagcat catattccag ccgacaaaat
      361 tctgtgaaac cgtcgcggaa atactttcat aacagcttaa ggcgttatag ctctctctgc
      421 tgtcggttgt acaagcatcg ccgtcagagt tggtccgata aaagcccaac gtcggccatc
      481 ccggattact ccagttggcc tgttgcatat cacctttgcc gccgtcatcg ttacggaaca
      541 cgctggcgct gattgcaaga ttccccaccc cgcctaaatc tgccgtccag ttattaccta
      601 actcgaaagc actcacatca tcggcattcg ccagccctgc gtagagctca tattgcggat
      661 gtaatggcag gcgtactcct agctgataag ctgaactttc atcatcagaa acctgtgatg
      721 tagttttacc tgcctgtaag aaccattgcg catttccgtc agtcagaccg ccggttttgg
      781 taaacggcac aagctcggtg cgggtgagtt ggttattttc gtagactttt aatgccacgc
      841 tgtagctgcc aggcggaaag gaactggtat caataaattg cgaaccacta ttgagataaa
      901 acgatcccaa aagttgctca ttacgataag cgtcaacacg agaattgcgc gaaagcagaa
      961 ccattactgg ggttccttgc tggctttgcg cctggtttaa atagctcagt gtcgacccga
     1021 tacgcatccc atcaattgca ccgagtggca gaaagttaaa ggtaaagttc ccgccttgtg
     1081 cattaaatag tgtgcggttg tccatgcgac ccgcctgcac ataataacga cgtaaaaaat
     1141 cataacgata atagagatcg ctgacgtcag cattactgtc actgacatca tctgcatcat
     1201 aaccgttgaa attccagtgt gcaccaatat agctattttc tgttattccc agcgcaccgt
     1261 ttccctgaat agacaacgat tggtaatcat cctgtgccag aacattgatg tcttgctgat
     1321 gtataaaagc attaacagta tttttgtctg gatttaaata taatgacttc gcatcgaaag
     1381 ctgaattcca ttgcggatta ataaacaacg ttgccgaact ttcttcatca ttatagatta
     1441 tttccagcgt atcggtattc agaaaaccac accctgagtc agtacgtaca taaccgcaag
     1501 ccaattcacc gtggcgagct agcggctgcg ataatttttc tttcactata tgcgcgattt
     1561 tctgatcgtc tacatccagc tccagcttgt tatatagact tgctggatca agaaatgtta
     1621 cggtatcaag attaaccatt gcataatatt taccgagact tttccctgaa aaataaacct
     1681 cgatatactc ctgctgtccc tgagcaataa cctcaaaccc tgcgggaatt ttggttaatg
     1741 tcgcctctgc cgggctgaga ctcgccagga tcaaaagagc cagtaatttt ttatccatgg
     1801 caatcctgaa atccataaca aatactaaaa cagttaaaat aagggagggc tacccctccc
     1861 tttgttacgt gttaattctg ttatctggat tagatatcct gagacagata aatgcttact
     1921 acaccacggt agatgcctgt ctccagaaca cctttggtgg tttgagcaaa catcaggttt
     1981 ttagccagag aaccgttagt taacgcatcg gaagcaaaga ttttggttgc ggtaaaagtg
     2041 gttgccgcat cagctttaat ttcttcaccg ccccaggtta ctgtcagcgg aacaatcttg
     2101 ctagcatcca ggctttgaac cagttgcgct ggggacacta actgcatttt tacatctttg
     2161 gtaacgtcgt tagaccagat tttggtcatc aactggtaag attgcagacc ctgccccggc
     2221 agatattgca tttctactgc tttcggcaac gcggtgttat cggtctgtgt catatccaga
     2281 gcggcatcaa cgttggccgt tacagtaata tccttttgta ctgccaatgc ggagcctgca
     2341 acagaaaaca tcgctgcgac cagaagagat tttgcaaata cctttttcat tactattcct
     2401 tcgtttatta caatgataat taatggaccg ccagttcaaa ctcgccatta tctttggtga
     2461 tccagttatt atatttgaca acgattttgt cgtagccaga tatgctttca agatttcttt
     2521 cctgaccagg gaagatgttt ttatttacgg tctgtatctt acaattttct ttgctattat
     2581 ttttacaata agcgatcgtt aaaatcttta agcgttgatt ccctgcatta actaattttt
     2641 gggcagcatt tacttctaac ttagcaatag gttgctgagg tggaacacta acaacaatcc
     2701 cccaaattaa gttaactgtt aactgcatag agagatcttt tttatctgtg gcaacgtcat
     2761 caatactggg aaccgcttca aatttaacgc gataattttt ctctttttgc tctggttcca
     2821 tcgcaacaaa acgtacggtt ttgctactac cggctggtaa agcgaattta ggtggcataa
     2881 caaccagatg atttgcatca ccggatttaa tctcaacttc attttcctga ggcgtcgatg
     2941 gattatcgat acggaatact gtcgccttga tgtactgaac ttcattactt ttagatatta
     3001 cgcgaacgtt gccttcaccc tgactattaa tggaaacagc cataggataa acagtcatat
     3061 ttgactgtgc caaaaaagga gcgaacacga ataaaagcgg cgtaagccgt gataagagat
     3121 taattttcgc caccatataa atttgatctt acaatattgt ttccaaaaag ttggtgtatt
     3181 agagatgtag atttattgta agtcaatata aaaaagcatt tttagttaat attaaattta
     3241 aaggctggga attaacttac tgaaatttag tagctttcac aatgacgata cttatttttt
     3301 aggaatttat aaatattcac aatattaatt caatacatta attcaatcac caaatttata
     3361 tatgaccaat ggcataccca tgaataaagt ggtatgataa cttcgtccgc attccactcc
     3421 gggagacgca tatggaaatc actgaaccac gccgtttgta tcaacaactt gccgctgacc
     3481 tgaaagagcg catcgaacag ggcgtctatc tggtaggtga taaactgcct gcagaacgct
     3541 ttattgccga tgaaaagaac gtcagccgta cggtagttcg tgaagccatc atcatgctgg
     3601 aagttgaagg ctatgtcgaa gtgcgtaaag gttcaggtat tcatgtggtt tccaaccagc
     3661 cgcgccatca gcaggcagct gacaataata tggaattcgc caattacggt ccgtttgagt
     3721 tgcttcaggc tcgccagctc atcgaaagta atattgccga attcgcggca actcaggtaa
     3781 cgaaacagga catcatgaaa ctgatggcca ttcaggaaca ggcgcgcggc gaacaatgct
     3841 tccgtgattc cgagtgggat ttacagttcc acattcaggt agccctggcg acgcagaact
     3901 ccgccctggc ggccatcgtt gaaaaaatgt ggacacaacg tagccataac ccgtactgga
     3961 aaaaactgca cgaacacatt gattcccgta ccgtcgataa ctggtgtgat gaccacgatc
     4021 aaatcctcaa ggcgctgatt cgcaaagatc ctcatgctgc taagctggca atgtggcagc
     4081 acctggaaaa caccaagatc atgttattca acgaaaccag cgacgacttc gagttcaatg
     4141 ccgaccgcta tctattcgcc gaaaacccgg tggtgcatct cgatacggcc accagtggca
     4201 gtaaatgaaa tacctgtccg acaggcgctg cgacgcgcct gtttgtctcg tgggtaagcg
     4261 aaaagtataa agtgtcagcc tgtgtaaatc ctctcgccac cctcccctgc attcagcaaa
     4321 atcagactcc acggacatgt aattttgata acgaacaacg ttgacctttg ttacaattag
     4381 attcaatttg aatttatgtt tttgaatgct ttcttatctc acgatttaac agggaatagt
     4441 tcaggctgtg ttgatgtatc aaacccgcag aacataccaa aacagcaata acattgcggt
     4501 agtgcatctt ttaaaaccag cgtggcgtta accgattcac caggaataat gaatggaact
     4561 tttgacccaa ttgctgcaag ccctgtgggc gcaggatttt gaaaccctgg ccaatccatc
     4621 gatgattggc atgttgtatt ttgtcttgtt tgtaattttg ttccttgaaa acggcttgct
     4681 tccagcggcc tttttaccgg gcgacagttt actggtattg gtcggcgtgt tgattgcgaa
     4741 aggcgcgatg ggttacccgc aaacgattct gctgctgacc gttgccgcca gcctcggctg
     4801 ctgggtcagc tatattcagg ggcgatggct gggcaatacc cgcaccgtac aaaactggct
     4861 atctcattta cccgcgcatt atcatcaacg cgcacaccat ctttttcata aacacggttt
     4921 atcggcgctg ttaattggtc gctttattgc gtttgtcaga acactgctgc cgacgattgc
     4981 cgggttatca gggctgaata acgcgcgctt tcagtttttc aactggatga gcggtctgct
     5041 gtgggtattg atcctgacaa ctctgggtta catgctcggc aaaacgccgg tatttttaaa
     5101 gtacgaggac cagctgatgt catgcctgat gctgctcccg gtggtgctgc tggtgtttgg
     5161 cctggcaggt tctctggtcg tgttatggaa aaagaaatat ggaaatcggg ggtaagggat
     5221 gcaaatacct cgcatgtcgc ttcgccagct agcctggtcc ggcactgttt tacttctggt
     5281 cggcacgctg ttgctggcct ggtccgcggt tcgccagcaa gagtctacgc tggcgattcg
     5341 tgccgttcat caaggcacaa cgatgccaga cggtttttcg atctggcatc accttgacgc
     5401 tcatggcatt cctttcaaaa gtatcacccc caaaaacgac actctgttaa ttacatttga
     5461 ctccagcgac cagagcgccg ccgcaaaagc ggtcctcgac agaacattgc cccatggcta
     5521 catcattgcg cagcaggaca ataacagtca ggctatgcag tggctgaccc ggttacggga
     5581 taactctcat cgcttcggat aacttcctgt tatccgaaac atatcactca ctttggtgat
     5641 ttcaccgtaa ctgtctatga ttaatgaggc ggtggttaac actaccgctt cgttgtctgg
     5701 acaatcgttc ctttgtaata ggtccgaatc acaatggaag gttcaagaat gaaataccgc
     5761 atcgctttag ctgtttctct ctttgctctt agtgccggta gttatgccac taccctgtgt
     5821 caggaaaagg agcaaaatat ccttaaggag atcagctatg ccgaaaaaca ccaaaaccag
     5881 aatcgtattg acggtctgaa taaagccctg agtgaagtcc gggccaactg ttcagatagc
     5941 cagctgcgtg ccgatcatca caagaaaatc gcaaagcaga aagatgaggt ggcggaacgc
     6001 cagcaagatt tagccgaggc gaagcaaaaa ggcgatgccg ataagattgc caaacgcgaa
     6061 cggaaactgg cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc
     6121 acaatagtca ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca
     6181 tctgcgtgct gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg
     6241 cgagaagtcg aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca
     6301 gagccgttat cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc
     6361 ggcgcgtgct gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggca ttggcgctgc
     6421 aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac
     6481 gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt
     6541 gaaatggtgg aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat
     6601 ctctttcaac ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg
     6661 agcctgatgg tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt
     6721 gccaccaccg tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa
     6781 tcgcgtaaat ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg
     6841 ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct
     6901 tagccagatc cagcaacaac ggctggatct ttccgccagt cgtcgtgaat ggctggaggc
     6961 aacaggcgct tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt
     7021 tggcagtagc gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc
     7081 cagacgcggt tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca
     7141 acagcttcgc ggttaacttc cctctggccg gagcaattcc ggccttatcc ctcaaatttt
     7201 ttgaagattt ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc
     7261 tccatcgaca gcaacgacgc taataccgcg cccttgcaca aaaaaacaat cagcagcctg
     7321 agtggcgcag tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcgc
     7381 attttaatgc cgattctgtt tattaccgca ggctggggaa aaattactgg ctacgcgggt
     7441 acccaacaat atatggaagc aatgggtgtc ccgggtttta tgctgccgct ggtgattctg
     7501 cttgagttcg gtggtggtct ggcaatcctg ttcggtttcc tgactcgcac cacagccctg
     7561 tttactgcgg gctttacgct gctgacggca tttttatttc acagcaactt tgctgaaggc
     7621 gtcaactcgc tgatgttcat gaaaaacctg acaatttctg gcggattcct gctgctggca
     7681 attaccggtc cgggcgcgta tagcatcgac cgcctgctga ataaaaagtg gtaagcacgc
     7741 gctatactta acgataaaaa acgaggagga agctcctcgt ttttgctatt ggaggagaga
     7801 aaaatgggtc aactgattga cggcgtctgg catgacacct ggtacgatac caaatctacc
     7861 ggcggtaaat ttcaacgttc agcttccgca tttcgtaact ggctcactgc cgatggcgca
     7921 cctggcccca ctggcacagg cggttttatc gcagagaaag atcgttatca tctctatgtt
     7981 tcactcgcct gcccgtgggc gcaccgcacg ctgatcatgc gcaaactcaa aggactggaa
     8041 ccgtttattt ccgtttccgt agtgaacccg ctgatgctgg aaaacggctg gacctttgat
     8101 gacagttttc cgggagcaac cggagatacg ctctatcaac atgaatttct gtatcagctt
     8161 tatctccacg ccgatccaca ctacagcgga cgagttactg ttcccgtgct gtgggacaaa
     8221 aagaaccaca ccatcgtcag caacgaatca gcagaaatca tccgcatgtt caataccgcg
     8281 tttgatgcgc tgggcgcaaa agcgggtgat tactacccac cagccctgca aacgaaaatt
     8341 gacgaactta acggctggat ttatgacacc gttaacaacg gcgtgtataa agctggtttt
     8401 gccaccagcc agcaagccta cgacgaggcg gtggcgaaag tgtttgaatc gctggcgcga
     8461 ctggagcaga ttttaggcca gcatcgttac ctgaccggca accagctaac cgaagccgat
     8521 attcgcctgt ggactacgct ggtgcgtttt gatccagtgt atgtgaccca cttcaagtgt
     8581 gataagcacc gcatcagcaa ttacctgaat ctgtatggct tcctgcgcga tatctaccag
     8641 atgccgggga tcgccgaaac tgtcaatttc gatcacatcc gtaatcatta cttccgcagc
     8701 cataagacca tcaaccctac ggggattatt tcaattggtc cgtggcagga tctcgatgaa
     8761 ccgcatggac gagatgttcg cttcggttaa aaaaaggcac cttacgaggt gcctctgata
     8821 attagctaag tgcatgttgt tcatgtcgga tgcggcgtga acgccttatc aaacctacaa
     8881 aacatttata ttcaatatgt tgcaatgacc gcgcaggcca gataagcgta gcgcatctgg
     8941 cgattttgcg cttgtcatca gtcttagcgc gccttcacag cactttatta attccttgaa
     9001 cgaatattta ctgccatttc attcacgtct attcttaatt tgctgcttta aaaacaagtg
     9061 attgagcaaa attgaggcaa aaatggactg gtatctgaaa gtactaaaaa attatgtcgg
     9121 tttccggggt cgtgcgcggc gcaaagagta ctggatgttt attctggtca acatcatctt
     9181 tacgttcgtg ctggggctgc tggataaaat gttaggctgg caacgcgctg gtggcgaagg
     9241 catcctgacg acaatctacg gtattctggt gtttttaccg tggtgggcag ttcagttccg
     9301 ccgcctgcac gacaccgacc gctcggcgtg gtgggcactg ttgttcttaa tcccgtttat
     9361 cggctggctt attatcatcg tctttaactg ccaggcgggt acgccaggcg aaaaccgctt
     9421 tggaccagat ccgaaactgg aaccttaaaa caaatctgct cgccggatgt ctgactcatc
     9481 cggcttttat catcatgttg atgaatgaat aatttactaa tgtttattta aaatatttca
     9541 atgtcgttat ataaattacc tataaaaaac aaccatgtat ttatcttaaa tatcaaaacc
     9601 tttataacat ccgaaaaaga aagatacgaa tagcatttac acaatacccg gaatagactt
     9661 tcgtcacttt taattaaagg gatgttttta tgcagtggta tttagccgta ttaaaaaatt
     9721 atgttggttt ctctggtcgc gcacgtcgta aagagtactg gatgtttact ctgattaacg
     9781 ccatcgtcgg cgctattatc aatgtcattc aattgatttt aggtttagag tttccattcc
     9841 tttctctcat ttacctggca gcaacaatca ttccggtcat tgctctgtgt gttcgtcgtc
     9901 tgcacgatac tgaccgttca ggtgcgtggg cgcttctcta tctggtaccg atcattggct
     9961 ggttagttct gtttgtattt gcctgtttag aaggcaattc tggcagcaat cgttacggaa
    10021 acgatcctaa gtttggttca aattaatctt agaattgggg cgacatttcg ccccttttta
    10081 ttaataataa caaatattat tttccgctaa aaagtttggg aatttcccgc agacaccagg
    10141 atttcgcttc ccccatactg tcacgacgcc aggccataat aatatcgatc tcgctggtcg
    10201 attccgggct gacgacacgc aaccgccctt ccgcaatatc tttttcgacc atcggatacg
    10261 gcatcgtcgc cacgccaagc cccgccagta atgcctgacg tttatcttca atcgtgctca
    10321 ccgttaagcg cggctgtttg tccagcagct gtacggtcaa caccgggcgc tcacgagcgg
    10381 tatccgctac cgcaattcca cgatatttca cgcgcgtcac ttcagataac ggttccggct
    10441 cctgatgaat cgggtgatca ggcgcggcaa cgtagacgtt cattaacgta tagagcttgc
    10501 gcgagttgat ctccgacgag gaacgaaaat gcatatccgg cgcgataaca atatccgccc
    10561 gcccctgctc cagtcgttcc cacgcccccg ccagcacttc ggtgatgatt gccagttggg
    10621 tattggcttt tgccgccagt ttgtcgatta acgggaaaaa ggcaggtgtc ggtaccagcg
    10681 cttcggtcac aatggtgaga tgcgtttccc aaccgcgcgc gagggcttcc gcatcggtag
    10741 tcagtttatc tgcggcttcc agcaaaacgc gccctcgctc cagcaacatc ctcccgacat
    10801 tggtgaattt ggtgcgatgg cccgagcggt caaacagcac cacatccagc tcttcttcca
    10861 gtttttgcat ggtgtagcta agtgcggaag gcacgcgtcc cagctcatcc gccgccgccg
    10921 caaaactgcc ccggcgatcg atcgcatcca taacccgtag tgcttccagc gttaatgccc
    10981 tttctttggc catttcgttc tcattcagga aatttgaaca taccgggcag aatatctggc
    11041 taacaatgta gcgtccagcc ccttaccata aaaggaagta aagagaggtc aagaattatg
    11101 attactaccc gaactgccag gcagtgtgga caagcagact acggatggtt gcaggcccgg
    11161 tatacttttt cctttggaca ctacttcgac ccgaaattgt taggctatgc ctccctgcgt
    11221 gtgcttaacc aggaagtgct ggccccaggt gccgcctttc agccgcgaac ctatcccaaa
    11281 gtcgatattt taaatgtgat tctggatgga gaagcagagt atcgcgacag cgaaggcaat
    11341 catgttcagg ccagcgccgg tgaggcgttg ctgctctcta cccagccggg tgtcagctac
    11401 agcgaacaca atctcagcaa agacaaaccg ttaacacgaa tgcagctttg gctggacgcc
    11461 tgcccgcagc gagagaatcc gctgattcaa aaactggcgc ttaatatggg caagcagcaa
    11521 ttaatcgcct cgccagaggg tacgatggga agcctgcaat tacgccagca agtgtggctg
    11581 caccatatcg tgctcgacaa aggcgaaagt gcgaatttcc agttgcatgg gccacgcgcg
    11641 tatttgcaat cgattcacgg gaaatttcat gcgcttacgc atcatgaaga gaaagcggcg
    11701 ctgacctgcg gtgatggggc gtttattcgt gacgaggcta acattacgct ggttgccgat
    11761 tccccactgc gcgctttgct gatagatttg cctgtctagt tgtttttaca gggagatgat
    11821 gatgagtaaa aaatcggcca aaaagcgcca gccagtgaag cccgtggtgg cgaaagaacc
    11881 tgctcgcacc gccaaaaatt ttggctatga agagatgttg agcgagctgg aagccatcgt
    11941 cgcggatgct gaaacgcgtt tagccgagga tgaagctacc gcgtaaagac aggttgccgg
    12001 atgcggcgaa aacgccttat ccggcctaca aaatccaaca acattaacga attaaacaac
    12061 ccgtaggccg gataagatgc gccagcatcg catccggcat caggtacaaa acacaacaac
    12121 atcaacaagt tgagcacccc gtaggccgga taaggtgcgc cagcatcgca tccggcacga
    12181 tacccaaaac ctggcgttta tctggccttg ctcgccataa tctcgataat ctgccgatcc
    12241 gtttgctgca tcgaatggct tgctaacgca cacaggttgg caatcgactg ctcaacatca
    12301 tgcgccacga tcccttcatt gccggtcacg gcggtatcat ccagcgccat taacaccgct
    12361 ttccacgcag ccgaagcact ggtcgaaacc ttcatcgcgc agctgttcga cgcaccatcg
    12421 caaatcatgc cgctgacatc gccgatcata ctgctgatcg ccatcgagat ggtttcataa
    12481 cgcccatcca ccagccatgc catcccggcg gcggccccca ttgctgcggt cgttgcggca
    12541 cacagcgcag acaaacgcgg taactggtta tggatgtaaa ttgcgctcaa atgagaaagc
    12601 atcagcgcac gcgccagccg ttcatcatcc gctccgaagt gttctgctac caccaccaca
    12661 ggcattgttg cggtgatccc ctggttaccc gagccggagt tactcatagc cggaagcgta
    12721 gcgccgccca tacgcgcatc ggatgccgcg ctggtacgaa tcacaatgga tgaagagaga
    12781 tctttcgcca gcaaaccgcg ctcgcactgt ttttccagcg tcgcgccaat atgcagcccc
    12841 cacttaccgc tcaaaccttc ctgcgataac gcacaattta gcttcgcgga atcgagaata
    12901 aagcggatcg ccgcaaacgg gacttcattg acgaacttca ggatctcagc cagcgtcgtt
    12961 ctggaaagca ccgtcagcgg agactcttgc tcgccctctg ccacacacgc ctgctgggta
    13021 aacaccacac cattgtgcgt ctcaatatgc acaatgttgg tatgcccgcc gacgatggtg
    13081 acacacgccc acttctcacc gttccagact ttggcgcgtg agaagaggat ttcattgcaa
    13141 ggttcctgga tcttaacgga gactttcccc gccgccagca gtgctttggc atcggcaatt
    13201 gcctgcgcag ttgcgtcttt cagcacttcc agcccggcgt tggcatttcc acctaacgcc
    13261 cccagcgccg ccgcaatcgg cagccccacc attcccgtgc cgggaacggt gacgcccaga
    13321 ccgttcttca tcagatttgg cgaaacccag gcttctacac gttcaaccgg accttccagt
    13381 tctgccgcag caaccgccgc cgccagcgcc agtgaaatcg gttcagtaca tcccagcgcc
    13441 ggttttactt cctcctgaac ggcgaggatg taacgctgcc ataacggatt taaagtcgaa
    13501 tcaaacatat taaaaacctt aaaatttcag gtaaatcaag aaaatgccag gaacggagaa
    13561 acacaaagca acaaaccagt gacgataatc aggtacagag acatcccttt gtatttgtgc
    13621 aatgccggta ctttgtaaac cagccacgcc gggatcaggt accctaccat gccgaaaatc
    13681 gggctacaga tagaggtgaa gctcaacacc ggtgcgttca gtacgatggc gctccaggcc
    13741 agcaaaatgg cgaaaatcat gatgccgcgc tgaacaagat tttcgttaat cttctcggca
    13801 ggcatcttgc gacgcaggat gttcattacg atcccttgcg ttgcttcgcg aaagcctaaa
    13861 tagacgccaa agaatgcagt catgacagca aagatattga gaatgacgct gaccactttc
    13921 acccacgctg cgccgtcacc gctaataaac tgtgcggcaa tcgccagcgc ggaaatattc
    13981 tgctcatagg cttttaccgc ttcgtcatgt cccatcgcca gcgtgaacga cacggcgtag
    14041 aaaaagacgg tgacaaacaa aatgccaaac gcgatattca ttgcccgcaa tgctttatgc
    14101 cgcgccactt caattgattt ttcccgcgag cgataagaga tcaccatcgg gcttaacgtc
    14161 tggataaaca gaatcgacgt cagggtaaac ggcagcgtaa taatggcgtt tttcaccagc
    14221 agccccagcg gcggtagcga accgacgtta tacagatgcc acattcccac catcgacacg
    14281 cccagcgccg cgaccaccag cagcttggtc agcaccatgc cggtcgaaat tttgaataac
    14341 aatttctcgc cgcgtgaaga gatcgccacc agaatgcaaa tcagtaccag accataaaag
    14401 ggactgtctg acagcaaccc ttccgtcacg ccgaaggtat gcagatagga agcactatcg
    14461 ttggtgatgg cggtggaata gacgaacatc caaatcacca gcattacgaa atagagcgca
    14521 cctaacagga tgccccagtt tttacctaaa taaccgctaa tgacactcgg gtaatcttta
    14581 cattctggtg attctgccag cgtattaata aacaaccgct gaaacagata cattgccggg
    14641 taaccaatca ccgatgagag caaaaatacc cacaatccca tcaaaccgac ctgcaccggg
    14701 agaaaaacaa tccccgcgcc aatcgccatc ccgatactca taatcaccca gccggtgtca
    14761 gtactgtcga atttgatcgc ttctcgccac tcgctctcac tcattccagc acgacccgcc
    14821 ggggtcgaag cgtctgcaat gacgccttta ttcgatgcaa tttccataat ttctcgctca
    14881 atattttgta gggcttatta tttttttccg agccgcatca aggcgatatg cggtctacgt
    14941 ttttgcaggc gagttattag aataaagaaa tgatacgcgc gaggtcagag aaaatcttca
    15001 caatcaagac tttgaaataa cggagatgga taagaatttt ctactaaatt aatcgcaaga
    15061 gaatattcat tctctatttg cggcagatca caaaaaaaag gtgcacattt gtgcacccaa
    15121 ggatgaaagc tgacagcaat gtcagccgca gaccacttta atggccagtc ctccgcgtga
    15181 tgtttcgcgg tatttatcgt tcatatcttt gccggtttca tacatcgtct cgatcacttt
    15241 atcgagtgaa acacgcggtg ccgaggtgcg gcgcatcgcc atccgcgcgg cgtttactgc
    15301 tttcacggca ttaatggcat tacgttcaat gcacgggatt tgtacctgtc cggcaaccgg
    15361 atcgcaggtc agcccaaggt tatgctccat cgcgatttcc gccgcattgc atacctgcgc
    15421 cggactgccg cccagtagtt cagttaaccc tgccgccgcc attgaacagg ccacgccaat
    15481 ctccccctga cagccgacct ccgcgccaga gatggaggcg ttcattttat acagcgcgcc
    15541 aatagccccc gctgccagaa aatagcgggc aattgaccgc tcgtttaccg gacgacggaa
    15601 cttatcgtaa taagccagca ctgccggaat aatgccgcac gcaccgttag tcggtgccgt
    15661 taccacgcgc ccgccagctg cgttttcttc actaaccgcc agcgcgtaca tgttgatcca
    15721 gtcgatgaca ttcatcggat cgttagagat gttatcgcta gaaaccagct gacgacgcag
    15781 cgctacggca cgacgcggca cattgagcgg accaggcagc acgccttcag tgttcatccc
    15841 gcgttcaata ccgtcatgca tcacttgcca gatacgggca aaccctgcgt caatttccgc
    15901 tttgctgcgc agcgccagct cgttgtgcat catcaggcca gatatagaca ggccgttgta
    15961 atcacacatt ttcagcagtt cacctgctga gtggaaatcg taaggtacgg acgtttcgac
    16021 atcgtgcgac aggccgaagt gttcttcttc gacaataaac ccgccgccga cagagtaata
    16081 ggttttactt aatagcgctt cctgtccctt ccaggcagtg atccgcattc cgttctcatg
    16141 gcgaggcaac atttcgggat ggaagataat gttctttgct acaggaaaat caacaatatg
    16201 cgcaccagat gccactggca gccgcccgct gcgcgttact aactctataa atgcagggat
    16261 ctcatcaatg acaacatcct gcggactgtt tcctgccagt cccatgataa tggcgacatc
    16321 cgtggcatgg cctttgcccg tcagtgacaa cgacccatac agatcgacca caatatggct
    16381 cgtcgcggtt aataagccgc tactttccag ccgatcaata aaactttttc cggcattcat
    16441 tggccccacg gtatgcgaac tggagggacc aatcccaatt ttgaaaatat cgaatgcact
    16501 aatcatatcc acaccctcgg attgccgttc agtgaagtgg agcggaacga ccttgcgacc
    16561 gtcccgctca cgaggcttta cgcactacgt actgcgatag cttcaatttc cagcttcaca
    16621 tctttcggca aacgcgcaac ctgcacacag ctccgggtcg ggtaagtcgc ctgatgctca
    16681 tcgaagaact gcttatagac ttcattgatg gtggcaaaat cattcagatc ggtgatgaac
    16741 acggtcatct tgatgatatc gcccacgctc agcccggcag caaccacgat cgctttgacg
    16801 ttttcgaggc ttaaacgcgc ctgatcttgc acatcagccg ggatctcacc ggtctgcggg
    16861 caaaccggaa tttgcccgga ggtgaagacc atgctgccta aatcaacgcc ctgaacataa
    16921 gggccgattg cgcctggggc acgttgcgtt tcgataatct ttttcataca tcctccggcg
    16981 tcagagcgcc tgggtaaaag tacgtgaaat aacatcctgt tgctgttcac gggtcagtgc
    17041 gttgaagcgc acggcgtagc cagagacacg gattgtcagg ttaggatatt tttccgggtg
    17101 ctcgatggca tccagcagca tttcccgatt cattacgttg acgttgaggt gttgaccgcc
    17161 ttcgacatcc gcttcgtggt ggaaataccc atccagcagg ccgacaaggt tggttttacg
    17221 tactggatct tctttgccca gcgccgcagg aacgattgag aaggtgtacg agatcccatc
    17281 tttggcgtag gtgaacggca gtttcgccac cgacgtcaat gaggccacgg cacctttgcg
    17341 gtcacgacca tgcatcgggt tagcgcccgg cgcgaacggt gttccggcgc gacgaccgtc
    17401 cggcgtatta ccggttttct ggccgtacac cacgttagaa gtgatagtca gaatcgactg
    17461 ggtagggacg gcgttgcgat aggttggcag cgctttaatt ttcttcataa agcgttcaac
    17521 caggtcgcag gcaatgctgt ctacgcgctc gtcgttgttg ccgtactgcg gatattcacc
    17581 gtcgatttca aagtccaccg ccaggccgtt ttcgtcacgg attggtttca cgcgggcata
    17641 tttgatggca gacagggagt ccgtcgccac cgacaggccc gcgatgccgc atgccatagt
    17701 gcgatagaca tcacgatcgt gcagcgccat cagcgaagct tcgtagctgt acttgtcgtg
    17761 catgtagtga atgatattca gcgcgctgat gtactgcacc gccagccagt ccatgaagtg
    17821 atcgaggctg tccatcactt tgtcgtaatc cagcacgtcg tccatcagcg gtgctgtttt
    17881 cggcccgacc tgaatcttca gcttctcgtc caccccgccg ttaattgcgt agagcagcgt
    17941 tttcgccagg ttagcgcgtg caccaaagaa ctgcatttgc ttaccaatca ccattgggct
    18001 gacgcagcag gcaatcgcgt aatcgtcgct gttgaagtca gtacgcatca gatcgtcatt
    18061 ttcatactgc aaggaagagg tgacgatcga cacctgcgcg gcatattttt tgaaggcaat
    18121 cggtaattct tccgaccaaa gaatggtcag gttaggttcc ggtgccggcc ccatagtgtg
    18181 cagggtgtgc aaatagcgga aggagttttt ggtcaccagc gtacgaccgt ccagccccat
    18241 cccgccgatc acttccgtcg cccagattgg gtcgccggag aacagcgaat caaattccgg
    18301 tgtacgcagg aagcgtacca tacggatctt catgatgaag tgatcgatca gttcctgtgc
    18361 ctgctgctca ttgagtacgc cagctttaaa gtcgcgctca atgtagatat cgaggaacga
    18421 tgccgtgcgg cccagcgaca tcgcgccgcc attttgcgat ttcactgccg ccagataagc
    18481 gaagtagagc cactgcaccg cttcctgcgc attctgcgcc gggcgagaga tatcaaagcc
    18541 atatttcgcc gccatttcct gaatctgcaa cagcgcatga cgatgctctg ccagctcctc
    18601 acgcagacgg atggtggctt ccagatcctc gcctttttcc agacgagact ggagatcggc
    18661 aaattgcagt tcgcgttcac gtaccagata actgatgcca tacagcgcta cgcggcgata
    18721 gtcaccgata atgcgcccac ggccatagcc atctggtaaa ccggtcagca cgccagattt
    18781 acggcagcgc agcatatccg gtgagtaaac atcaaatacg ccctggttat gggttttacg
    18841 cagatcggta aacagatatt caaattcact gtccatttca cggccatagg cgtggaatga
    18901 acttttaatc atattaatgc caccgaacgg gtgtagcgca cgtttcaacg gcgcatccgt
    18961 ttgcaggcca acaatttttt ccagcggctg gttaatatat cccgcatcat gagcggtaat
    19021 tgtggtggca atattggtat cgaaatcaac cggcgcgtgg gttgcgtttt cgatacggat
    19081 accttccatt actttttccc acaattccgt ggtggcaggc gtcgcttcgg cgaggaaaga
    19141 ttcatcgcct tcatacggtg tatagttatg ttgaataaaa tcgcggacat taatttcgtt
    19201 tttccagtcc gtacctttaa agccaagcca tgcgtcggcg tacagcttat cgctggtatc
    19261 aatatctacc ttcatgaaaa ataatctctc tacaatactt caactaaatt atgcaaattc
    19321 tgcgggcgcg ttaactttgc ctaaatgaat ggcatccaaa gcaatcattt tttcttcgtt
    19381 agtcggaata acagcgcaaa tgacacgcgc attttcactg gaaacaattc gctcaccaca
    19441 ggagttagag cgattattca tttctgtatc aatctctacg cctaatacag ccaaatgttc
    19501 catgaccaga cgacgaatta agcttgaatt ctctcctatt ccgccggtga atataattcc
    19561 atccaggcga cgtaatgaag ctgcgtgtcc ggcaatatga cgggcaattc ggtgaacaaa
    19621 ggttttaatt gccagttgcg cgcgttcgtg accttcatgc caggcttttt ccagaacacg
    19681 taaatccgaa gaaagaccgg aaatacctaa taatcccgac tctttattca ctacgcgttc
    19741 caggtcaccc aggctctggt tggtttggct ggcgacccag gacatcgcac caaagtcgac
    19801 atcgccactg cgggtaccca tcatcaagcc ttccagcggc gtcattccca ttgaagtatc
    19861 aacactctga ccgttgcgaa ccgcgcagat tgacgcgcca ttgccaagat gcgccacaac
    19921 caggccggag tcatcttccg ccagattcag cagcgaatgt gcgcgctggg aaacatagcg
    19981 gtgcgacgtg ccgtggaaac cataacggcg tacacctaac tcttcataat atttccacgg
    20041 caggccgtat aaataagctt ccggagccat cgtctggtgg aaactggtat caaataccgc
    20101 cacctgagtt acgcccggaa ataattgctg cgccgattca ataccactta aattggcgta
    20161 attatgcagg ggtgccagtg gagaaacgcg acggatatta tcaatgactt catcggtaat
    20221 aatggcggac tcggtaaaaa tactgccgcc gtgagcgatg cggtggccaa ttaaggccac
    20281 actgtcattt aaattccgtt tttccagttc aaatgcaatt gccttcaatg caccttcgta
    20341 gctgtggtga gccagcggtg ctggctctcc cccatttacg gataagaatg cattttccga
    20401 gttaataccg tcggcaatac ctgacattaa tacttcacag tcgctggcat cgagtacgga
    20461 aaacttaatc gaagacgaac cacagttaat aaccaaaaca accggaaatt cattcatctc
    20521 ttttctcatc ctgagttacg gattaaaaca gtttgtatac gatgttcagg atggtcagca
    20581 gaccaatcac ggtaacaaac acgttatcca gacgaccacg gtatttcgcc agagacggcg
    20641 ctttacggat ggcatacatc ggcaacaggc acagcaggga tgcgataatc ggtgcgccca
    20701 tggcttcaat aaggtcgagg atgttcgggt tggcgtaggc aacaacccag gtggagccca
    20761 tgatgaagat catgctgata gtgttcagtt tacccagcga cactttagtt ttgtcgcctt
    20821 tataaccgaa cttcagaatc agaccattca agccttccag cgtccccaga tagtgaccga
    20881 agaaagattt gaagatagcc acgagtgcga tgatggaagc cgcatattcc agtgtaattg
    20941 cgaacgttgt tttggtaccg gtcatggacg caaagtggtt agccagataa gaaagcactg
    21001 gaatattctg cgctttggct tccgccatgt tggccggaga cagcgtaaac aggcagctaa
    21061 aggcaaagaa catcaccact gcaaccatca gcatgctggc acgagaaatg atttgggaac
    21121 atttacgttc ggtgaagtcg cgaccgaagt ctttctcata ctcttcacgt ttagaaacca
    21181 cgaaggaaga gacgattggc gagaagttaa aggagaaaac catgatggaa atccccagcc
    21241 agacagtgat caggataccg tcatgaccgg ttaacgacag cgaaccgagg tcaacctggt
    21301 cgataactgc agagttccag taagggatca gcgacagaga aatcagcacc aggctggcga
    21361 taaacggcca taccaggtag ctcatcactt taaccatcag atccttacca aaccagatga
    21421 cgaaagccat cagcagcaac aggaacagcg ccacaaagcc gcgattcagc ggtgcaaatc
    21481 cgagctggtt ttcccagaac gtcataaagg tattggtaat agtaacgcca taaatccaca
    21541 gcagtgggca aatcgcgaag aagtacagga acgtgataac cacgccgcca gttttaccaa
    21601 aatgctcttc caccgtttcc gtaatgttgc cggaagggtt agagccagaa agacacagac
    21661 gcgccagcgc ccggtggcaa taaaatgcga tggggtatgc caataccaac atcagaagaa
    21721 tcgggatcag tccgccaaaa cctgcgcgga tagggaagaa cagcaccccg gcgccgattg
    21781 ccgtaccaaa caagcctaac gtccatgtgg tatctgattt acgccaggac gattgttttg
    21841 tctggctgga tacaatgcta tctgaagtac tcatatccta tcctcaacga attaattaag
    21901 cgtcaacgaa accggtgatt tgagagacgc gagaaagatc gatattgccg ccggaaataa
    21961 tactgacggt ttttctgt
//
LOCUS       DAMZET010000066        21936 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.066, whole genome shotgun sequence.
ACCESSION   DAMZET010000066 DAMZET010000000
VERSION     DAMZET010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.066"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(4..79)
                     /locus_tag="POB34_002528"
     tRNA            complement(4..79)
                     /locus_tag="POB34_002528"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44..46),aa:Val,seq:tac)
     gene            complement(126..201)
                     /locus_tag="POB34_002529"
     tRNA            complement(126..201)
                     /locus_tag="POB34_002529"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(166..168),aa:Val,seq:tac)
     gene            complement(246..321)
                     /locus_tag="POB34_002530"
     tRNA            complement(246..321)
                     /locus_tag="POB34_002530"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(286..288),aa:Val,seq:tac)
     gene            580..1995
                     /gene="gltX"
                     /locus_tag="POB34_002531"
     CDS             580..1995
                     /gene="gltX"
                     /locus_tag="POB34_002531"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDI5919457.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(2047..2439)
                     /locus_tag="POB34_002532"
     CDS             complement(2047..2439)
                     /locus_tag="POB34_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDI5919458.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(2441..2800)
                     /locus_tag="POB34_002533"
     CDS             complement(2441..2800)
                     /locus_tag="POB34_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDI5919459.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            3021..3096
                     /locus_tag="POB34_002534"
     tRNA            3021..3096
                     /locus_tag="POB34_002534"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3054..3056,aa:Ala,seq:ggc)
     gene            3136..3211
                     /locus_tag="POB34_002535"
     tRNA            3136..3211
                     /locus_tag="POB34_002535"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3169..3171,aa:Ala,seq:ggc)
     gene            3419..5608
                     /locus_tag="POB34_002536"
     CDS             3419..5608
                     /locus_tag="POB34_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HDI5919460.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMINILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(5658..6860)
                     /gene="nupC"
                     /locus_tag="POB34_002537"
     CDS             complement(5658..6860)
                     /gene="nupC"
                     /locus_tag="POB34_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HDI5919461.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            7196..8434
                     /locus_tag="POB34_002538"
     CDS             7196..8434
                     /locus_tag="POB34_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDI5919462.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            complement(8575..8901)
                     /locus_tag="POB34_002539"
     CDS             complement(8575..8901)
                     /locus_tag="POB34_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDI5919463.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(9016..10272)
                     /locus_tag="POB34_002540"
     CDS             complement(9016..10272)
                     /locus_tag="POB34_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HDI5919464.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            10476..11441
                     /gene="glk"
                     /locus_tag="POB34_002541"
     CDS             10476..11441
                     /gene="glk"
                     /locus_tag="POB34_002541"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HDI5919465.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHNNPGLLGSGAHLRQTLGHIL"
     gene            11660..11986
                     /locus_tag="POB34_002542"
     CDS             11660..11986
                     /locus_tag="POB34_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HDI5919466.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            12008..13255
                     /locus_tag="POB34_002543"
     CDS             12008..13255
                     /locus_tag="POB34_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDI5919467.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESRITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            13270..14354
                     /gene="ypdF"
                     /locus_tag="POB34_002544"
                     /pseudo
     CDS             13270..14354
                     /gene="ypdF"
                     /locus_tag="POB34_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            14354..15391
                     /gene="ypdE"
                     /locus_tag="POB34_002545"
     CDS             14354..15391
                     /gene="ypdE"
                     /locus_tag="POB34_002545"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDI5919468.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            15416..17911
                     /gene="ptsP"
                     /locus_tag="POB34_002546"
     CDS             15416..17911
                     /gene="ptsP"
                     /locus_tag="POB34_002546"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDI5919469.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFFNHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(17914..18771)
                     /locus_tag="POB34_002547"
     CDS             complement(17914..18771)
                     /locus_tag="POB34_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI5919470.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(18784..19518)
                     /gene="ypdB"
                     /locus_tag="POB34_002548"
     CDS             complement(18784..19518)
                     /gene="ypdB"
                     /locus_tag="POB34_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HDI5919471.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(19533..21230)
                     /gene="ypdA"
                     /locus_tag="POB34_002549"
     CDS             complement(19533..21230)
                     /gene="ypdA"
                     /locus_tag="POB34_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HDI5919472.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            21607..>21936
                     /locus_tag="POB34_002550"
     CDS             21607..>21936
                     /locus_tag="POB34_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDI5919473.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEG"
BASE COUNT         5218 a         5446 c         5841 g         5431 t
ORIGIN      
        1 aagtggtggg tgatgacggg atcgaaccgc cgaccccctc cttgtaaggg aggtgctctc
       61 ccagctgagc taatcaccca cttcggtact tcacattttt acaagaaatt tagctggcga
      121 gaaagtggtg ggtgatgacg ggatcgaacc gccgaccccc tccttgtaag ggaggtgctc
      181 tcccagctga gctaatcacc cacttctcaa tttcttgcta cacggcggag actacataaa
      241 gtagttggtg ggtgatgacg ggatcgaacc gccgaccccc tccttgtaag ggaggtgctc
      301 tcccagctga gctaatcacc cccgctgtgt ggagtcgcat tatagggaga gttcaaaatg
      361 agtcaacgca ttttctaaag aaattgttcg ttcgtcgtaa atttaagcaa gatgatcgca
      421 aaacagaccg tgttgcgcaa tttgtcaacg aaaacaataa tgcgtaaggt agaaacccga
      481 actacattga ggaatcaggc gggagtgata gaatatcgcc cactcaattt ttccaggatt
      541 tgccggttgt cggcatcttt ctaaacgtaa ggccatttca tgaaaatcaa aactcgcttc
      601 gcgccaagcc caacaggcta tctgcacgtt ggcggcgcgc gtactgctct ttactcctgg
      661 ctttttgcac gtaaccacgg cggtgagttc gtgctgcgta ttgaagacac cgatcttgag
      721 cgttccacgc cggaagctat cgaagccatt atggatggca tgaactggct gagcctggag
      781 tgggatgaag gtccgtacta ccagaccaaa cgttttgatc gctacaatgc ggtgatcgat
      841 cagatgctgg aagagggcac tgcttataaa tgctattgct ctaaagagcg cctggaagcg
      901 ctgcgcgaag agcaaatggc gaaaggtgag aagccgcgtt atgacggtcg ctgccgccac
      961 agccatgagc atcatgctga tgatgaaccg tgtgtcgtgc gttttgctaa tccgcaggaa
     1021 ggttctgttg tttttgacga tcagatccgt ggtccgatcg agttcagcaa ccaggagctg
     1081 gatgatctga ttatccgccg taccgatggt tccccaacct ataacttctg tgtggttgtc
     1141 gatgactggg atatggaaat cacccacgtt atccgtggcg aagaccatat caacaacacg
     1201 ccgcgccaga tcaacattct taaggccctg aaagcgccgg tgccggttta cgcgcacgtt
     1261 tctatgatca atggcgatga cggtaaaaaa ctgtccaaac gtcacggggc agtcagcgta
     1321 atgcagtatc gtgatgacgg ttatttgcca gaagcactgc tgaactatct ggtgcgtctg
     1381 ggctggtccc acggcgatca ggaaatcttc actcgtgaag agatgatcaa atacttcact
     1441 ttgaatgccg tcagcaaatc tgccagtgcg ttcaacaccg acaagctgct gtggctgaac
     1501 catcactaca ttaacgcgct gccgccggag tatgttgcta ctcacttaca gtggcacatt
     1561 gagcaggaaa atatcgatac ccgtaacggc ccgcagctgg ctgatctggt gaaactgctg
     1621 ggcgaacgct gcaagacgct gaaagagatg gcacagagct gccgttattt ctacgaagat
     1681 tttgctgagt tcgatgccga tgcggcgaaa aaacatctgc gtccggtagc gcgtcagccg
     1741 ctggaagtgg ttcgtgacaa actggccgcg attactgact ggaccgctga aaacgttcat
     1801 cacgctattc aggcgacggc ggatgagctg gaagtgggta tgggtaaagt tggtatgccg
     1861 ctgcgtgtcg ccgtaaccgg tgcggggcag tctccagcac tggatgttac cgttcacgca
     1921 attggtaaga cccgcagtat cgagcgtatc aacaaagcgc tggattttat tgctgaacgc
     1981 gaaaatcagc agtaattagc gtgtaaaaag ataaacggca ggagataata tcctgccgtt
     2041 ttttatttat gctgattcgt caatatcgag acgggcgagg aaattgcgaa ttccttcacg
     2101 tgacaaaaat tgcgcgactt tttcctgctc aatgtctgtc atattatcta tcgcgttgat
     2161 gatttgtcga tacgccgggg cgcgtgctcc aggagcgtat tcagcaattc tctcttcagc
     2221 ttccatcaac attggtgtgt tatggaaggc tggcgtgttc tgaataaact cgcaaacttg
     2281 cgtatcgacc agaataagtc tggcacgacc gcctttcacg ccgggaaatt tttccgtttt
     2341 ccagcctttt tctctgatcc aacggttaac agtttgttta gccacaccga ggcattcagc
     2401 cagttcttcg gtggtcattt tgttgcgtaa tcttttcata ttatttgcta tcgcgaatcc
     2461 ctaagcgttg taacaatccg ataatccctt cccgcagaag caaggatgta aactgttttt
     2521 gttcaactgg cgtcatctct ttcgccagtg tcaccagcaa tacttcaagc ggcgcatcac
     2581 cagaaagggc aggcgcttct ccctgacctt ctggacgttc agcattgcga atatattcac
     2641 gaacctgttc attgacgtga accagtcggg ctttgccacc ctggacgcca ggttttggtg
     2701 acgttgtcca gccttccttg cgtacccatt tattaatggt ctggcggcta tagccggtca
     2761 gtctggcaag ttcatctggt gtcatcctct ccttgaacat aacaattccc tgaatgtatg
     2821 tgggtctatt agtagttcat tttataacac cactttacag gaaaccgtga catgaataac
     2881 tactgaatgc gagcggtttt ccaaaattgt tcatttgcct gatttttcag cgattgaaac
     2941 tacacgttgc aatttgccgt tgacacattc gggcggaatt catatgatgc cgcccgtcaa
     3001 ctcgacaagc tttacgtgac ggggctatag ctcagctggg agagcgcttg catggcatgc
     3061 aagaggccag cggttcgatc ccgcttagct ccaccaaatt ttgcacccag caaatttggt
     3121 acgtaaacgc atcgtggggc tatagctcag ctgggagagc gcttgcatgg catgcaagag
     3181 gtcagcggtt cgatcccgct tagctccacc aaatttccaa ccctcgctgc aaagcggggg
     3241 ttttttgtct ctgctttttg ccgcttttgt aatacagtct acgtccgggt tagcgccgcc
     3301 tggtgaaagc atcattggat gaaaaatcgg caacaggctg gcccctgttt gcttcgcgat
     3361 gcgaataaac ttattatttg tgtgcctgaa aaccccgatc agtgagagta gtgtactcat
     3421 gtttgtggag cataacctga taaaaaatat caagatattc acactagcgt ttacgctcac
     3481 cgtggtactt attcagctat cccgttttat ttcgccactt gccattatcc attccagtta
     3541 tatctttctg gcgtggatgc cactgtgcgt aatgctgtca atcttgttta tctttggctg
     3601 gcgcggtgtc gttcccgttt tatgcgggat gttttgcacc aatctgtgga actttcatct
     3661 ctctttttta cagactgcgg tcatgctcgg tagccagacg tttgtcgtgt tgtgtgcctg
     3721 cgcaatatta cgctggcagc tggggacgcg ttggcgttat ggattgacca gccgatatgt
     3781 ctggcaacgt ctgttctggc ttggtttggt gacgccgatc ggcatcaaat gcagcatgta
     3841 tcttgtgggg agcttctttg atttcccgct aaagatatcc acctttttcg gcgatgcgga
     3901 tgccattttc acggtcgttg atttgctaag ccttttcacc gctgtgctga tttacaacat
     3961 gcttttctac tatctcactc gcatgattgt aagtccccac tttgcgcaga tattgtggcg
     4021 cagggatatc gctccgtcgt tgggcaaaga gaaacgcgca tttaccttaa gctggctggc
     4081 agcgttaagc gtgctgctac ttctgttgtg cacaccttat gaaaacgact ttattgccgg
     4141 ttacttggta cccgttttct tcatcatctt taccctcggg gtcggtaagc ttcgctatcc
     4201 gtttttaaat ctcacctggg ctgtttcaac gctttgcctg ctgaattaca accagaactt
     4261 tttgcaaggg gtggaaaccg aatattcgct ggcatttatt cttgcggtgc tgatttcctt
     4321 tagcgtttgc ctgctctata tggtgcgcat ttatcatcgc agtgaatggc ttaatcgccg
     4381 ctggcatttg caggcgctga cagatccgtt aacgctccta cccaactttc gtgcgttgga
     4441 acaagcgccg gagcaagagg cgggcaagag tttttgctgc ctgcgcattg ataatcttga
     4501 gtttatgagt cgtcattacg gcttaatgat gcgcgttcac tgtatccgct caatttgccg
     4561 tacgctgctg ccgttgatgc aggaaaacga aaagttgtat caattgccgg gtagtgaact
     4621 gctgttagtg ctgagcgggc cggaaacgga agggcgactc cagcatatga ttaacatcct
     4681 gaatagtcgg caaattcact ggaacaatac cgggctggat atgggctatg gtgctgcctg
     4741 ggggcgtttt gatggaaatc aggaaaccct gcaacccttg ttggggcagt taagctggct
     4801 ggcggagcaa tcctgcgcac atcatcatgt gctggcgctg gatagcagag aggagatggt
     4861 ttccgggcag accactaaac aggtgctatt gctgaatacc attcgcacgg cgttagatca
     4921 gggtgatttg ctgctctacg cccagccaat tcgcaacaaa gagggtgaag gttatgatga
     4981 gatcctcgcg cgactgaaat atgacggcgg cattatgacc ccggataagt ttctgcccct
     5041 tattgctcag tttaacctta gcgcgcgttt tgatttgcaa gtgctggaat ccttgttgaa
     5101 gtggctggca acacaccctt gcgacaaaaa aggaccgcgc ttttcagtca atttaatgcc
     5161 gctcacgctg ctgcaaaaga atattgccgg gcggattatt cgtctgttta agcgttatca
     5221 catctccccg caggcggtca ttcttgagat caccgaggag caggcgtttt ctaacgcaga
     5281 aagcagcatg tacaacatcg agcagctgca taagtttggt ttccggattg cgattgatga
     5341 ctttggcacc ggatatgcca actacgaacg gttaaagcgt ttgcaggctg atatcatcaa
     5401 aattgatggc gtctttgtga aagatattgt cacgaacacg ctggatgcga tgattgtgag
     5461 atcaattacc gatctggcga aagcgaagtc attgagtgtg gtcgcggagt ttgtcgagac
     5521 gcaacagcag caggcgctat tgcataagct cggggtgcaa tatctgcaag ggtatttgat
     5581 tggtcgcccg cagccattag ctgattaaca ggcgtaaaaa aaccggggaa ttatccccgg
     5641 cttttttatg tatggtttta cagcaccagt gctgcgattg acgcagacag cacactcacc
     5701 agggtagagc cgtaaaccag tttcagaccg aagcgagaaa ccacgttacc ttgctcttca
     5761 ttcaggcctt taactgcacc tgcgataatc ccgattgaag agaagttagc gaaggaaacc
     5821 aggaacacag agattatgcc ttcagcacgc ggagagagcg tggaagcaat tttctgcaga
     5881 tccatcatcg caacgaactc gttggaaacc agtttggtcg ccatgatact gcccacttgc
     5941 agtgcttcac tggaaggaac acccatcacc catgcaatcg gatagaagat gtagcccagg
     6001 atgccctgga aggagatgct gtagccaaac cagccagtaa cggtggcaaa cagtgcgttc
     6061 agcgcggcga tcagggcgat aaagccgatc agcatcgcgg caacgataat ggcaactttg
     6121 aaacctgcca gaatgtattc acccagcatt tcgaagaagc tctgaccttc gtgcaggttg
     6181 gacatctgga tgttttcttc actggcatca acacggtaag gattgatcag cgacagcacg
     6241 ataaaggtgc tgaacatgtt cagtaccagc gcagcaacga cgtatttcgg ttccagcatg
     6301 gtcatgtatg caccaacgat ggacatcgac acggtggaca ttgccgtggc agccatggtg
     6361 tacatacgat tacgggagat tttgccgagg atatctttat aggcaataaa gttttcagac
     6421 tgacccagaa tcagggagct gacggcgtta aaggattcca gtttgcccat accgttgact
     6481 ttggagagca ggaaaccaat tgcgcggata ataaccggca acacgcgaat gtgctggaga
     6541 ataccgatca gtgcagagat aaagacgatt gggcacagca ctttcaggaa gaagaatgcc
     6601 aggccttgat cattcatgct accaaagacg aagttagtcc cttcgttggc aaatccgagc
     6661 agtttttcga acatttcgga gaagcctttc acgaagccta aaccaacgtc ggagttcagg
     6721 aagaaccacg ccagtaacac ttcgataaca agcagttgaa taacataacg gatacgaatt
     6781 tttttgcggt cgctgcttac cagcagtgcg agaatcgcaa caacggcaag tgccagtaca
     6841 aaatgaagga cgcggtccat atttgctcca aatatgaggc aggttaaatt tccgtgcaca
     6901 ttctatgtaa catggttaag gaaaacgaga tctgacacac actataatga cgtcctgtga
     6961 caagcttcaa aaatagtgat ctgtcataca tttctgctat gttgtgtatg gaagctgaaa
     7021 gttatgtaaa tgtgctaaca ttaaacataa ccataacatt caagtcatca atcttttcgt
     7081 agtcataatc ctggtctatc aaagaaatca ccacaatcca ttcaaatgaa tttgataatc
     7141 attctcgttt ggcatagcat gaaacatagc aaaggctatg ttttagaggc acaagatgac
     7201 gaactatcgc gttgagagta gcagcggacg ggcggcgcgc aagatgaggc tcgcattaat
     7261 gggacctgcg ttcattgcgg cgattggtta tatcgatccc ggtaactttg cgaccaatat
     7321 tcaggcgggt gctagcttcg gctatcagct actgtgggtt gtcgtttggg ccaacctgat
     7381 ggcgatgctg attcagatcc tctctgccaa actagggatt gccaccggta aaaatctggc
     7441 ggagcagatt cgtgatcact atccgcgtcc cgtagtgtgg ttctattggg ttcaggcaga
     7501 aattattgcg atggcaaccg acctggcgga atttattggt gcggcgatcg gttttaaact
     7561 cattcttggt gtttcgttgt tgcagggcgc ggtgctgacg gggatcgcga ctttcctgat
     7621 tttaatgctg caacgtcgcg ggcaaaaacc gctggagaaa gtgattggcg ggttactgtt
     7681 gtttgttgcc gcggcttaca ttgtcgagtt gattttctcc cagcctaacc tggcgcagtt
     7741 gggtaaagga atggtgatcc cgagtttacc tacttcggag gcggtcttcc tggcagcagg
     7801 cgtgttaggg gcgacgatta tgccgcatgt gatttatttg cactcttcgc tcactcagca
     7861 tttacatggc ggttcgcgtc aacaacgtta ttccgccacc aaatgggatg tggctatcgc
     7921 catgacgatt gccggttttg tcaatctggc gatgatggct acagctgcgg cggcgttcca
     7981 cttttctggt catactggtg ttgccgatct tgatgaggct tatctgacgc tgcaaccgct
     8041 gttaagccat gctgcggcaa cggtctttgg attaagtctg gttgctgccg gactgtcctc
     8101 aacggtggtg gggacactgg cggggcaggt ggtgatgcag ggattcattc gcttccatat
     8161 cccgctgtgg gtgcgtcgta cagtcaccat gttgccgtca tttattgtca ttctgatggg
     8221 attagatccg acacggattc tggttatgag tcaggtgctg ttaagttttg gtatcgccct
     8281 ggcgctggtt ccactgctga ttttcaccag tgacagcaag ttgatgggcg atctggtgaa
     8341 cagcaaacgc gtaaaacaga caggctgggt gattgtagtg ctggtcgtgg cgctgaatat
     8401 ctggttgttg gtggggacgg cgctgggatt gtagttgaat gagcgtcgca tctggcacta
     8461 ttggcggatg cggcgtaaac gccttatccg ccctacgcgt taaagagccg gtttgtaggc
     8521 ctgataagac gcgctagcgt cgcatcaggc attgtgttcc attggcgaat gtgtttaatg
     8581 gcggtgtcca tgtccgcggc cttttccgcg atgatcgtca cgatcgcgcc agccttcacg
     8641 atagccacgc tcatacgctt tacgcttatc ccagccacga tgatagccat tatcatgacg
     8701 ccaccagcgg tttttacgcc actcataatt gcgatgccag tagtcacggt cgcgccagtg
     8761 accaccgtcc cagtaattac cgtaatgatc gcgatcgcca atttgtaatt tgattgatgg
     8821 cagtagggtg atttcacctg cgtttgctgc aagcggggta aatgccatca gggcggccgc
     8881 cagaaacagt gacctgaaca ttgttattct ccttcacgat cgaagccgtc agcggcctgt
     8941 taacgcaata ttacggggag gtaaagcccc gtatcatcgc cataactctt aaatcataaa
     9001 ggagagcatt ttttgctaaa cgttatcgtg gggtggctgt tgcttcggac gattgaccat
     9061 catcatcggc ttacctgcta ataacagcca tgccgggagc atgacgatac agagcagcgg
     9121 tagcaatgtg gtattgggta caacgaccgc cgccataaaa agacttaacc agccatcgcg
     9181 tgttaccacc agcacgatgc cgagaatagc gcaagaaacg gttattgccg ctggtacggc
     9241 gggtacgtgc tcatgcagca tcaaccctaa tgccacgccg acaaacactg ccgggaagat
     9301 tcgcccaccg cgaaagccac tggcggcagc aacgaccagg gcggcaagtt taattaccgc
     9361 cagcaaaaag taatcgctgg tgctgaaagc ctgatttgcc accatctgct gcatctcatc
     9421 cagcccttta aacagcgaaa ctggtccacc aataaccccc agaataccga gaataaatcc
     9481 gccaataccc agcacgagca ccggattttt catttgattc atcatcgcgt gcaaccgtgg
     9541 taagcaccat acggcgacca ttcctgctgc gatggcaatc gccgcgacaa ttgcaccgct
     9601 gagaatatcg gtcatttcca tctgtccgta atgcgcaatg ggcagtgaaa aatggggatg
     9661 gaaaaataat ccggtagtaa gtgcaccagc tgctgccgcc attaatggcg caaaaagacg
     9721 atcccatagc ggaacttcac tactgccatt taaggtttgc gaaaatatca acgccgccgc
     9781 aacaggtgta ccaaacagcg cgccgatggt tccggcagag gctaaaatag tccactccat
     9841 tcggttgacg cgcggtaaca gacgagcgcc aatggcaacc gcaagggcga tattgacggt
     9901 catgatcgga tgttccggcc ccaggctgac gccgccagca agaccgagaa ttaatgctac
     9961 gataagtcct ggtagcgcag agggcggaac tggtgcgccg atcagcggtt cacaggcggg
    10021 gtctggtccg gcatgaccct ggctgaaacg gataaccaac cccaccgcaa tacccgttag
    10081 cgttaataca ccgatgatcc agagaggtga atcctgggct atccccagag ttcccggcag
    10141 tcgttgccag agcaaattct gtaataccga ggcgattttc atcaccacaa tcagaataag
    10201 actggacgca atcccaattg ccacggcggg gagcgataat aacaacatgg ttctggctcg
    10261 cggatggagc atgttctatt ccttatgcgg ggtcagatac ttagtttgcc cagcttgcaa
    10321 aaaggcatcg ctgcaattgg tgctgaaacg ataaagtaaa tgtgtgaccc agatcgatat
    10381 ttacagggag cctgcctttc cggcgttgtt gttatgcccc caggtattta cagtgtgaga
    10441 aagaattatt ttgactttag cggagcagtt gaagaatgac aaagtatgca ttagtcggtg
    10501 atgtgggcgg caccaacgca cgtcttgctc tgtgtgatat tgccagtggt gaaatctcgc
    10561 aggctaagac ctattcaggg cttgattacc ccagcctcga agcggtcatt cgcgtttatc
    10621 ttgaagaaca taaggtcgag gtgaaagacg gctgtattgc catcgcttgc ccaattaccg
    10681 gtgactgggt ggcaatgacc aaccatacct gggcgttctc aattgccgaa atgaaaaaga
    10741 atctcggttt tagccatctg gaaattatta acgattttac cgctgtatcg atggcgatcc
    10801 cgatgctgaa aaaagagcat ctgattcagt ttggtggcgc agaaccggtc gaaggtaaac
    10861 ctattgcggt ttacggtgcc ggaacagggc ttggggttgc gcatctggtc catgtcgata
    10921 agcgttgggt aagcttgccg ggcgaaggcg gtcacgttga ttttgcgccg aatagtgaag
    10981 aagaggccat tatcctcgaa atattgcgtg cggaaattgg tcatgtttcg gcggaacgcg
    11041 tgctttctgg tcctgggctg gtgaatttgt atcgcgcaat tgttaaagcc gacaaccgcc
    11101 tgccagaaaa tctcaagcca aaagatatta ccgaacgcgc gctggctgac agctgcaccg
    11161 attgccgccg tgcgttgtcg ctgttttgcg tcattatggg ccgttttggc ggcaatctgg
    11221 cattaacgct gggaaccttt ggtggggttt atattgctgg cggtatcgtg ccgcgcttcc
    11281 ttgagttctt caaagcctct ggtttccgtg ccgcatttga agataaagga cgctttaaag
    11341 aatatgtcca tgatattccg gtgtatctca tcgtccataa caatccgggt cttctcggtt
    11401 ccggtgcaca tttacgccag accttaggtc acattctgta aatccttcct gttatatcgg
    11461 gaggtaactc tcccgataat cttttaaatc atacagttaa ttcaattttt ctttgtgtcc
    11521 cctcacaagg ccgacctgcg tcacacttcc gtacagcggg attaattctc cagtaaatgc
    11581 attatttgtc tggtaacggc gatttgtttt gcacgttcat aatttcactc gtcaactatc
    11641 actgaatgag gaattaacga tgagtaagaa actgattgcc ttatgtgcct gcccaatggg
    11701 cctggctcac acctttatgg ccgctcaggc gttggaagaa gcggcggtag aagccggtta
    11761 tgaagtgaaa attgaaaccc agggcgcgga cggtatccag aatcgcctga cggcgcagga
    11821 tatcgccgaa gcaaccatca tcatccactc cgtggcagtt accccggaag ataatgagcg
    11881 tttcgaatca cgcgacgttt atgaaatcac tttgcaggac gcaattaaaa acgctgcggg
    11941 catcatcaaa gaaatcgaag agatgattgc ctctgaacaa cagtaatatt cgacagggat
    12001 aattgttatg gccattaaaa aacgcagtgc aaccgttgtg cctggcgcat ccggtgcggc
    12061 agcggcagtt aagaatccgc aggcctctaa aagcagcttc tggggtgaac tcccgcagca
    12121 tgtgatgtca gggatttcac gcatggtgcc aaccttaatt atgggcggtg tgatcctcgc
    12181 tttcagccag ctgattgctt atagctggct taaaattcct gctgagatcg gcatcatgga
    12241 tgctcttaat agcgggaaat tctccggttt cgacctctcc ttacttaaat ttgcctggct
    12301 gtcgcagtcc ttcggcggcg tgttgtttgg ttttgccatt ccgatgtttg ctgcttttgt
    12361 ggcgaactct atcggtggca aactggcgtt cccggctggt tttatcggcg ggttgatgtc
    12421 tacccagccg acgcaactgc tgaacttcga tcccagcact atgcaatggg cgacctcttc
    12481 gccggtgccg tcaaccttca ttggtgcgct gattatttct atcgtcgcgg gttaccttgt
    12541 gaagtggatg aaccagaaaa tccagttacc ggatttcctg ttagcgttca aaaccacatt
    12601 tttgctaccg attctttccg ccatttttgt catgctggcg atgtactacg tcatcacccc
    12661 ctttggtggc tggatcaacg gcggtatccg tactgtgctg actgccgcag gtgagaaggg
    12721 cgcgctgatg tacgcaatgg ggatcgccgc ggcgacagca atcgaccttg gtggcccgat
    12781 caacaaagcc gcaggtttcg ttgccttcag ctttaccact gaccacgtat taccggtcac
    12841 cgctcgttct atcgctatcg ttattcctcc gattggtctg ggtctggcga ccattattga
    12901 ccgtcgttta accggcaaac gcctgttcaa cgctcaactt tatccgcagg gtaagaccgc
    12961 gatgttcctt gcctttatgg ggattagtga gggtgcgatt ccgtttgcgc tggaaagccg
    13021 catcaccgcc attccgtcgt atatggtcgg cgcgattgtc ggctcaaccg ccgctgtctg
    13081 gctgggcgca gtgcaatggt tcccggaatc cgctatctgg gcatggccgc tggtcactaa
    13141 cctgggcgtc tatatggcgg ggatcgcgct gggggcagtc ataactgcgc tgatggtggt
    13201 gttcctgcgt ctgatgatgt tccgtaaagg caaattgtta atcgatagcc tgtaagaagg
    13261 aaaatccaga tgacattact cgcttcgctg cgcgactggc ttaaggcgca acaactggat
    13321 gcagtgcttc tctcctcacg gcagaacaaa cagccgcatc tggggatctc caccggacag
    13381 gctatgtggt tattagccgt gaaagtgcgc acattctggt agattcgcgc tatttcgttg
    13441 aggtggaagc ccgtgcgcaa ggctaccagc tgcatttgct tgacgcgacg aacacgctta
    13501 ccactatcgt caatcaaatc attgccgatg agcagttgca aacgctcggt tttgaaggcc
    13561 agcaggtgag ttgggaaacc gcgcaccgct ggaagtctga actcaatgcg aaactggtta
    13621 gcgccacgcc ggatgtgctg cggcaaatca aaacgccaga ggaggtggag ataatccgcc
    13681 tcgcctgtgg gattgccgat cgcggagcgg agcatattcg ccgctttatt caggcgggga
    13741 tgagcgagcg cgagatagcc gctgaactgg agtggtttat gcgccagcag ggcgcagaaa
    13801 aagcctcttt tgacaccatt gtcgccagcg gctggcgcgg ggcgctgccg cacggcaaag
    13861 ccagcgacaa gattgttgca gcgggcgagt ttgtcactct cgattttggt gcgctgtatc
    13921 agggctactg ctctgatatg acgcgcacct tgctggtgaa tggcgaaggg gtgagcgccg
    13981 aatctcaccc gctgtttaac gtctaccaga ttgtcctgca ggcacagctc gcagcaatct
    14041 ctgcaattcg ccccggcgtg cgctgccagc aggttgacga tgccgcgcgc caagtgatta
    14101 ccgaggcagg ttttagccac tatttcggtc ataacaccgg tcatgctatc ggcattgaag
    14161 ttcatgaaga tccgcgtttt tcaccgcggg acaccacgac gctacagcca ggcatgttac
    14221 tgaccgtgga gccggggatt tatttgccag ggcaaggggg cgtgcgcatc gaagatgttg
    14281 tgctggttac cccgcaaggc gcagaagtgc tctacgccat gccgaaaaca gtgttgctca
    14341 cgggagaggc ataatggatt tatcgctatt aaaagcgttg agcgaggcag atgcgatcgc
    14401 ctcctcggaa caggaagtgc ggcagatcct gctggaagaa gcggatcgcc tgcaaaaaga
    14461 agtgcgattt gatggtctgg gatcggtgct gatccgcctc aatgaatcga caggtccgaa
    14521 ggtgatgatc tgcgcccata tggacgaagt gggatttatg gtgcgtagca tctcccgcga
    14581 aggggcaatt gatgtgctgc cggttggcaa cgtgcgcatg gctgcccgcc agctgcagcc
    14641 ggtgcgcatc accacccgtg aagagtgcaa aattccaggc ctgcttgacg gcgaccggca
    14701 ggggaatgac gtcagcgcca tgcgcgtgga cattggtgcg cgctcgtatg acgaagtgat
    14761 gcaggcggga attcgtccag gcgatcgcgt cacctttgat acaacttttc aggttctccc
    14821 tcaccagcgg gtgatgggga aagcctttga tgatcgcctc ggttgctatc tgctggtgac
    14881 gttactacgt gagctgcacg atgccgaact gcctgcggag gtgtggctgg tggcaagttc
    14941 cagcgaagag gtgggattac gcggtgggca aaccgctacc cgcgcggtgt cgccggacgt
    15001 cgccattgtg ctggataccg cctgctgggc gaaaaacttt gattatggcg cggctaacca
    15061 tcgccagatt ggtaacggcc cgatgctggt gttaagcgac aagtcactga ttgcgccgcc
    15121 aaaacttacc gcctggatcg aaaccgtggc ggctgaaatt ggcgtaccgt tgcaggcaga
    15181 tatgttcagc aacggcggca cggacggcgg agcggtgcac ttaaccggta ctggcgtacc
    15241 cacagtggta atggggcctg ccacccgcca cggacattgc gcggcatcga ttgccgattg
    15301 ccgcgacatt ttgcagatgg agcaactttt atctgccctt attcaacgtc ttacgcgtga
    15361 gacggttgtt caactgacgg atttcagatg atctcctgat taacccggag cggttatgtt
    15421 aacgattcaa tttctctgtc ctttgcctaa cggtctacat gctcgtccgg cgtgggaact
    15481 taaagaacag tgcagccagt ggcaaagcga aatcaccttt ttcaaccatc gccagaacgc
    15541 aaaggccgat gctaaaagct cgctggcgtt gattggaact ggcaccttat ttaatgacag
    15601 ttgcagcctg aacattagcg gcagcgatga agagcaggcg cggcgcgtgc tggaagagta
    15661 catcctggtg cgctttatcg acagcgacag cgttcagccg acgcaggcag aactgacggc
    15721 gcatccgctg ccgcgttcat taagccgcct taacccggat ttactgtacg gcaatgtgct
    15781 ggcaagcggc gtcggcgtgg gtacgctgac cctgttacaa agcgacagcc tcgacagtta
    15841 tcgggcaatc cccgccagtg cgcaagattc cacccggctg gagcacagcc tggcaacgct
    15901 tgccgagcaa ctgaaccagc aattgcgtga gcgtgacggc gaaagcaaaa ctatcctcag
    15961 cgcccatttg tcgctgattc aggatgatga attcgcgggc aatatccgtc gtctgatgac
    16021 agaacagcat caagggctgg gggcggcgat catcagcaat atggagcagg tttgcgccaa
    16081 actttctgct tctgccagtg attatctgcg cgaacgtgtt agcgacattc gcgatattag
    16141 cgaacagttg ctgcatatca cctggccgga actgaagccg cgcaacaatc tggtgctgga
    16201 aaaaccgact attctggtag ccgaggattt aaccccaagc cagtttttga gcctcgattt
    16261 aaaaaatctt gcaggcatga ttctggagaa aactgggcgc acctcgcata ccctgattct
    16321 ggcccgcgcc tcggcgatcc cggtactgag cggcctgccg ctggatgcga ttgcccgtta
    16381 tgccgggcaa ccggcggtgc ttgacgccca gtgcggcgtg ctggcgatta acccgaatga
    16441 cgcggtgagc ggttattatc aggtcgcaca gacgctggcg gataaacgcc aaaaacaaca
    16501 ggcgcaggcc gccgcgcagc tggcctattc ccgtgataac aagcgtattg atattgcggc
    16561 gaatatcggc accgctctgg aagcgccagg cgcgtttgcc aacggcgcgg aaggcgttgg
    16621 actgttccgt actgaaatgc tgtatatgga tcgtgacagc gcgccggacg agcaggagca
    16681 atttgaagcc taccagcagg tactactggc agcgggcgac aagccgatta tcttccgcac
    16741 aatggacatc ggtggcgata aaagcattcc ttatctgaat attccccagg aagagaaccc
    16801 gttcctcggc tatcgcgcgg tacgtattta cccggaattt gctggcctgt tccgcactca
    16861 actacgggcg attttgcgcg ccgccagttt tggcaacgcc cagttgatga tcccgatggt
    16921 ccacagtctc gatcagatct tatgggtgaa gggcgagatc caaaaagcga tcgttgagct
    16981 taagcgcgat ggcctgcgtc atgcagagac gattacgctg gggatcatgg tggaagtgcc
    17041 gtcggtgtgc tacatcatcg atcacttctg cgatgaggtc gatttcttca gtatcggctc
    17101 caacgatatg acccagtatc tgtatgcggt cgatcgtaat aacccgcgcg tatcgccgct
    17161 gtataacccg attacgccat cgttcctgcg catgttgcag cagattgtta ccactgcgca
    17221 tcagcggggc aaatgggtag gcatttgcgg tgaactgggc ggtgaaagcc gttatctgcc
    17281 gctactgctt gggctgggcc tggatgagct gagtatgagt agcccgcgta ttccggcggt
    17341 gaaaagccag cttcgtcaac tggatagcga ggcgtgtcgg gaactggcgc gtcaggcatg
    17401 tgaatgccgc agtgcgcagg aaattgaagc gttactcacc gcctttacgc cggaagaaga
    17461 cgttcgccca ctgctggcgc tggagaatat ctttgttgat caggatttta gcaataaaga
    17521 gcaggcgatc cagttcctgt gcggcaacct cggcgttaac gggcgcactg aacatccgtt
    17581 tgagctggaa gaagatgtct ggcagcggga agagattgtc actaccggcg ttggttttgg
    17641 cgtggcgatc ccgcacacca aatctcagtg gatccgtcat tccagtatca gcattgcccg
    17701 gctggtgaaa ccggttgact ggcagtcaga gatgggcgaa gtcgaactgg tgatcatgct
    17761 gacgttgggt gctaacgaag ggatgaacca tgtgaaagtc ttctcgcagc tggcgcgtaa
    17821 actggtgaat aaaaacttcc gccagtcatt gtttgccgcg caagatgcgc aaagcatcct
    17881 gacgctgctg gaaacggaat taaccttctg acgttagccc tgaaaacggg cgctgtactc
    17941 tcccggcgtc agaccaaact gacgccggaa aacgcgacaa aaatagtcgc tgtccggaaa
    18001 accgcaacgc tgtgccactt catgaattga cagatgatat ttctgcaaaa tcatcctcgc
    18061 cttcgccatt cgcacccaac gcacatactc gataaaacgc atcgttccat gctgagcaaa
    18121 cagttttgac agatgattgg gcgtgatatt aaaaaactgc gccacgctct cgcgggtgag
    18181 cggctgggca taattatcct gaacccagtt acaaatgctg tgatagagaa aatcgccgcg
    18241 cggttgcgaa ttacccggcg gcatattcac cacgttgcgg caaagatgca gcaaacttaa
    18301 caccagtggc tgaatgatat tctgctccag cggcgaacgg ctaagatggg tgagggcggt
    18361 aagcatcgct tcaccttcac cgcgttgcag gctgggaagc tggatctggc gagcaggatg
    18421 caacaacgac gtggtgcggc tatcgtaaaa cgataaccca agccaggtcg gtgcgaacac
    18481 caggcttaac agcatcaccg gtttgttgtt gatcggtaaa ttagccgcgc gtgccggaat
    18541 aaacagcatt tctccttcca ccattcgctg ctgctttgct tccagcaggt tgccgtattc
    18601 gccgcgcagc acgatatcca gacggggaaa atcaatacat aaactgccca ccggtaacga
    18661 ggcaggctgg ctggcaaacc agacgcgccc cagtagttgc gggttaagca ccaggccgct
    18721 gaacagatcg gcgaaaaatt gttgatcggc gggcaagcct ggtgctttca tgaaagttcc
    18781 cgattaaaga tgcattaact ggcgaaattc tttcactttg ctgcggctga ccggcacttc
    18841 aaaatccaga tctttcagtc gcagaatgta ggtgttatta aaccacggtt cgatttcgcg
    18901 tattttgttc agattgacac aaaatgagcg atggcagcgg aaaaaatgcg acggcggcag
    18961 tttgctgcaa aattcggtaa tgttcatcgg cattacgtag gattcacgcc gcgtatagac
    19021 aaaggtcatt ttctcgtgcg cttcggcgta atagatatcg ttaattggcg tgacgattat
    19081 tcgctcatct ttcaccagat taatggtgtc attctcacgc gttaccgtcg cggcaggcgt
    19141 actgctggtc tgctgttgtt gccaggccgc ttccagtttt tgcagcatcc cggtaatacg
    19201 tgactcctga tacggtttga gaatgtagtc aaacgcctcc agttcaaacg cttctaccgc
    19261 atgttctttc cacgcggtga tgaacacaat aaacggttta tgggcgaact ggctgatgtt
    19321 ttgcgccagc aacacgccat ccagcgacgg aatattgata tccagaaaaa tggcgtcgac
    19381 gcggttatgc tgcaaaaact tcaacacgtc cagaccgtcg tcaaaggtgc cgacaatctc
    19441 catctggctg tgctctttaa ttagccagct cagttcctgt tgtgccagga attcgtcttc
    19501 aacaatgatg actttcacaa tatcactccg gctcaaagca ataacgtagc ctgtgaggcg
    19561 actggggtgc gttggttagg aatgtaaaac gcaatttccg tccccggctc caggcggcgg
    19621 atatgcagcc cctcgccata caataacttc acgcgatgat ggacattcag caggccaatt
    19681 ttattgcccg gcatttcatt cgcttcgacc cgctcaatca cctttggatc gatgccgtgc
    19741 ccggtatctc gcaccgcaat gcgtacccga tttccgcact ctgcaacgct gatggtgaca
    19801 acgcctttac ctttgcaagg ctgaataccg tggacaatgg cgttctccac caacggctgg
    19861 atcagcaggc tgggaatgca gcaattcacc tcttcatcaa tatcatagat aaccgtcagc
    19921 ttgtcaccaa agcgggcctg ctcaatggca atatagtctt taatttgata cagctctttt
    19981 ttgatatcga tttgctcatc gtcttttaat tcaatgttat agcgcagata acgcgacaga
    20041 ttaaagatca actggcgagc ggtatccgga ttcagacgga ttgacgatga aatagcgttc
    20101 agagcgttaa acagaaaatg gggattaatt ttgctttgca gggcgcgaag ctctgcctta
    20161 tttgccattt cacgtagctg ctcggcgcgt gaaacctcca gttgcgtgga gatgatttgc
    20221 gacagaccga ccgccatttc ctgtaatgac gaggtgatct gatgcgcgtg gcagtagtaa
    20281 attttcagcg ttccggtcac gacccctttc tcccacaatg ggatcaccag catggaatga
    20341 atctctggtg tgcggtgggc ttcatcattg tttttaatga tgatttttcc gtaattcatc
    20401 gcctgacggg tagtcgggct aatgaagtca tcgccattct gatagttatg ttcacccacg
    20461 ccaacatagg ccagcacatg atcggtattg gtaattgcca ccgcatcggc gtgaatgtca
    20521 tcgcggataa tttcgcagac cttgcgtaat gactcgctat tgacatggcg aaacagcggt
    20581 agcgttttgt tggcgatatc cagcgccagc ttggcctgcc gcgcggcgct ggcctctttt
    20641 tcgccctcaa cgctttgcac cagaagcaca ataaagccga tacagacgct accgaggatc
    20701 attggaatgc cgattttaga gacgatatcg atccccaacg cggtagttgg tgcccagaca
    20761 attaccagaa tcatggtcaa cgtctcacac aacatgccgc cgagaatacc gacgcgccag
    20821 cgctgtgctt tggggatttt caggttgatc cagcccgata tacaacccgc cagaatgctg
    20881 gtgataaagc aggggatcgc cgtcacgccg ccgatatcaa ttaaataccg gtgaataccc
    20941 gcaatcacgc cggtaatgat gcctacccac gggccaaaca gaatcccgcc ggacatcacc
    21001 gcgataatac gtacgttcac cagcgagcct tctacgggaa cgccggacca ggtgctgaac
    21061 agggcgaaca gcgagaaaat ggcggtaacg gcgagcaatt cttttgggga gtgcgccgac
    21121 ttgtgcaaca gttcgcgaaa caggcggata cggatgagaa agaacaggca gataagcatt
    21181 aacgctgccc gatcgaagac cgccagcagc atgttgaata tttcgtgcac gggtgactct
    21241 gaataaggat taaacctcta tgataggtaa cctgaaggct gatgaccagc aggccgtttt
    21301 tgaggaaaaa ccataaattt aaaaggggat tgaaagataa atggtgtatt agcaatgtat
    21361 ctcttgataa tgcacttctg gctggtaaat ttattgaaat tataatgtta ataaaattgg
    21421 tggtaatttt cttgttagcg tagaaaaatt tcctgttttt gaagtggagt tcctcattta
    21481 aagccattga taaaaatgtt ctaaaacgct ggacagacag aaattaatca ggctattttc
    21541 aaaacagcca ccattgtggc agcgtcgctc ggacggtccg ggcgctaacg ttaatctgag
    21601 gaaattatgg ctgacactcg ccctgaacgt cgctttacgc gcattgatcg tctcccgccc
    21661 tacgttttta acatcaccgc tgaactgaaa atggctgcgc gtcggcgcgg cgaagatatt
    21721 atcgatttca gcatgggtaa cccggacggt gcgactccgc cgcatatcgt cgaaaaatta
    21781 tgtactgtgg cccagcgtcc ggacacgcat ggttactcca cttcacgcgg cattccgcgg
    21841 ttacgtcgcg ccatttcccg ctggtatcag gatcgctacg acgttgaaat cgacccggaa
    21901 tcagaagcca tcgtcactat tggttcgaaa gagggc
//
LOCUS       DAMZET010000067        21806 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.067, whole genome shotgun sequence.
ACCESSION   DAMZET010000067 DAMZET010000000
VERSION     DAMZET010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.067"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(29..928)
                     /gene="nhaR"
                     /locus_tag="POB34_002551"
     CDS             complement(29..928)
                     /gene="nhaR"
                     /locus_tag="POB34_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HDI5919474.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(994..2160)
                     /gene="nhaA"
                     /locus_tag="POB34_002552"
     CDS             complement(994..2160)
                     /gene="nhaA"
                     /locus_tag="POB34_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HDI5919475.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            2746..2898
                     /gene="mokC"
                     /locus_tag="POB34_002553"
     CDS             2746..2898
                     /gene="mokC"
                     /locus_tag="POB34_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HDI5919476.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3002..4132)
                     /gene="dnaJ"
                     /locus_tag="POB34_002554"
     CDS             complement(3002..4132)
                     /gene="dnaJ"
                     /locus_tag="POB34_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDI5919477.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4221..6137)
                     /gene="dnaK"
                     /locus_tag="POB34_002555"
     CDS             complement(4221..6137)
                     /gene="dnaK"
                     /locus_tag="POB34_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDI5919478.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQARNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLIEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6514..6918
                     /locus_tag="POB34_002556"
     CDS             6514..6918
                     /locus_tag="POB34_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HDI5919479.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            6944..7657
                     /gene="msyB"
                     /locus_tag="POB34_002557"
     CDS             6944..7657
                     /gene="msyB"
                     /locus_tag="POB34_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HDI5919480.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            7806..8372
                     /gene="satP"
                     /locus_tag="POB34_002558"
     CDS             7806..8372
                     /gene="satP"
                     /locus_tag="POB34_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HDI5919481.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8407..8994)
                     /gene="mog"
                     /locus_tag="POB34_002559"
     CDS             complement(8407..8994)
                     /gene="mog"
                     /locus_tag="POB34_002559"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HDI5919482.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9109..10062)
                     /gene="tal"
                     /locus_tag="POB34_002560"
     CDS             complement(9109..10062)
                     /gene="tal"
                     /locus_tag="POB34_002560"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDI5919483.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10341..11771
                     /locus_tag="POB34_002561"
     CDS             10341..11771
                     /locus_tag="POB34_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDI5919484.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            11841..12617
                     /gene="yaaA"
                     /locus_tag="POB34_002562"
     CDS             11841..12617
                     /gene="yaaA"
                     /locus_tag="POB34_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDI5919485.1"
                     /translation="MLILISPAKTLDYQSPLTTMRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(12770..13066)
                     /locus_tag="POB34_002563"
     CDS             complement(12770..13066)
                     /locus_tag="POB34_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDI5919486.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13280..14566)
                     /gene="thrC"
                     /locus_tag="POB34_002564"
     CDS             complement(13280..14566)
                     /gene="thrC"
                     /locus_tag="POB34_002564"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDI5919487.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(14567..15499)
                     /gene="thrB"
                     /locus_tag="POB34_002565"
     CDS             complement(14567..15499)
                     /gene="thrB"
                     /locus_tag="POB34_002565"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDI5919488.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15501..17963)
                     /gene="thrA"
                     /locus_tag="POB34_002566"
     CDS             complement(15501..17963)
                     /gene="thrA"
                     /locus_tag="POB34_002566"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HDI5919489.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLTAAQPGFPLAQLKSFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(17989..18108)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18044..18109)
                     /gene="thrL"
                     /locus_tag="POB34_002567"
     CDS             complement(18044..18109)
                     /gene="thrL"
                     /locus_tag="POB34_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HDI5919490.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18323..19009)
                     /locus_tag="POB34_002568"
     CDS             complement(18323..19009)
                     /locus_tag="POB34_002568"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDI5919491.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19409..19549)
                     /locus_tag="POB34_002569"
     CDS             complement(19409..19549)
                     /locus_tag="POB34_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919492.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            19645..20361
                     /gene="arcA"
                     /locus_tag="POB34_002570"
     CDS             19645..20361
                     /gene="arcA"
                     /locus_tag="POB34_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HDI5919493.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20420..21772)
                     /gene="creD"
                     /locus_tag="POB34_002571"
     CDS             complement(20420..21772)
                     /gene="creD"
                     /locus_tag="POB34_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HDI5919494.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEDDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
BASE COUNT         5315 a         5732 c         5282 g         5477 t
ORIGIN      
        1 ccaccttaac tttgggggga ggtgccgatt aacgcgccgc tggactaaaa agcgcagaat
       61 aatccgtatt gcagattcgc tgtaccgccg ggtgctgaat catccgctca gcaaaaatag
      121 catggtactc ttccatcaca ttctcgacgc gaccaatttc tacgacagtt ttatcggcat
      181 aaaagtcata tgcataaagc gttggggcaa cgaagattgc attgtgcatc gcaccaaaag
      241 ctttcatcaa cgcggcatca tcaaactcgc cgaggatttc tacgtttaat ccctgggagt
      301 taaaccagtt aagcaatttg cgccctaaca ttgaacgtcg cccaggaatc aaaagtcgcc
      361 gttcttccag acaagccggg aacggttttt ctggtggtgg atttgtacac cagaaactca
      421 cgccacattc gccaattctc acggagaaca ggccttcctg ctgcgtagag tctatcggac
      481 agtcagaaat gatcatatcc agtttatgct gacttaattg ctccagcagc atttcgtggg
      541 tggattcgaa gcagcgaaga tgaatgggct cgccttctac cactgcggcg ttaagtacgc
      601 tactgaccag gcgtttggaa agtgcatcag ccacaccaac gtcaaacaat aaattggatt
      661 ctttgcgata gttcacaata tccagcattt cctggcttaa ggtgaacatt ttatcggcat
      721 agcgatagac cagttctccc agctcgctgg gttcgagacc acgtcccttg cgtttaaata
      781 atttgccttg caggcgctct tccagcgctc gaatctgtcc ggtaatggtt tgtggagtta
      841 aataaagcgc ctccgctgcg ccaaccacgg aaccttcttt atagacatgc cagaagtaat
      901 acaagtggtt gtaattgata tgagacatgc tcatttctct ccctgataac aatgaaaagg
      961 gagccgttta tggctccccg gtaaaccgtc ctgtcaaact gatggacgca aacgaacgcg
     1021 taaccagctg tatccaatta ccgccgaaga gatagaaccg accaggatac cgagtttcgc
     1081 ccagttaatc agttctggat ctacgctacc aaaggccagg ctggcaataa agatagacat
     1141 agtaaaaccg ataccgcaca ggatccccac cgccataatt tgctgataag tcgttccctc
     1201 aggcagatgc gccagtttca aacgcagcgc caaccagcag aacagactaa tccccagcgg
     1261 tttgccaatc agcaagccag cgatgatccc caatggcaga atggaggtca agccatccag
     1321 cgtgacacct tgcagtgaaa cgccagcatt agcaaatgca aacagcggca aaatcagata
     1381 cgccacccac gggtgtaaca catgctccag tcgcttcgct ggagaacgcc catgcttctc
     1441 tttcaaagga ataaagaagc cgacaattac ccccgccagg gttgcgtgaa cccccgattt
     1501 caacaccgca gtccacaaca ctacgccaac cagaatatag acgcccgtgc ggcgtacacc
     1561 acacagattc aataccgcga gtaccgcaat tgctacagcc gcgacgccaa gagaggccat
     1621 cgataagtca ttagtgtaga acaatgcgat gataatgatg gccccaagat cgtcgataat
     1681 agccagagcc atcaaaaaga tcttcagcgc taacggaaca cgacttccca acagcgccag
     1741 cacaccaagt gcaaaggcaa tgtcagtagc cgccgggatc gcccaccctt cgcgggtaat
     1801 cggatcggca tagttaaagg ccagatagag taatgccgga acaatcatcc caccaatagc
     1861 ggcgataact ggaaatgcgg cctggcgtaa gctggctagc gatccttgca tcagttcacg
     1921 tttaacttcc agaccgacta acaggaaaaa taccgccatc agcgcgtcat ttatccataa
     1981 cagcatgttt ttgttgattt cgagtgaacc aacccggagc tgaaccggcg tctccagaaa
     2041 gtcgtgatac catccactgg ttgcgccgct attggccata atcatcgcca ggatagcggc
     2101 aatgataaga ataatgcctc ccgaggcatc actgctaaag aatcgatgca gatgtttcac
     2161 tttttatttc tctttcaggt gaatagatcg tttttactat tttaccccgc acaaatcatc
     2221 gttaaaatca gattatagtg taggaataac tcgtttttta cgatccattg aagaatttgc
     2281 gccatcagcg gtgagatgag tttttgacgg ctgtaagcgc tcacgagtga ccctaagccc
     2341 agataatcat agcttaaggt gaggatttct gagagattaa ttaggaaaag taataattaa
     2401 tttgttgtca gtcggcgatg tttgttaggc caatatttca aaaataatcc agcttgtcgt
     2461 caataattgc gatgaagaga taaattaaag ttaaataatt aaatcattaa aaaattacgc
     2521 cataaaaatg cgaggcgtct tcaggattag aatcgataaa acagaatggg ttaaagagag
     2581 cgatataata gcggcgggtg cttgaggctg tctgtctcag gcattagctg aacggcagat
     2641 agagaaaagc cccgagtgat attttaccat caacccgagg ccccctatat gctgaacaca
     2701 tgtagagtgc ctcttactga ccgtaaggtc aaggagaaga gagcaatgaa gcagcataag
     2761 gcgatgattg tcgccctgat cgtcatctgt atcaccgccg tagtggcggc gctggtaacg
     2821 agaaaagacc tctgtgaggt tcacatccga actggccaga cggaggttgc tgttttcacg
     2881 gcttacgaat ccgagtaaga gcaacggcgg ggagtgatcc ccgccacttt acaggtgctc
     2941 gcatatcttc aacgcaccct atttttaccc aggcctgccc acgggcaggc ttttggggag
     3001 gttagcgggt caggtcgtca aaaaacttct tcacaccatc aaagaagctc tttgagcgcg
     3061 ggctgttgtg ctcgccggtt gggccaccga agctttcttg cagctcttgc agcagctgtt
     3121 tctgcttctc gttcaggcct accggtgttt cgacgacaac gcggcacagt aaatcaccct
     3181 gtgcgccacc gcggacagac ttgacgcctt taccgcgcat acggaacagc ttaccagtct
     3241 gggtttcgcc aggcactttc agtttgacgc gaccatcaag ggtcggtact tcgatttcgc
     3301 cacccagcgc cgccatagcg aagttgatcg ggacttcgca atacaggttg ttgccttcac
     3361 gctcgaaaat cgggtgctgt ttaacctgaa cctgaacgta cagatcgcct gccggtgcgc
     3421 catgctcgcc cgcttcacct tcgcccgcaa gacggatgcg gtctccagtg tccacccctg
     3481 ccgggatttt aacggacagc gttttgctgc gctcaacacg accatgacca tgacatttgt
     3541 tgcacggatc tttgatcagc gtaccgcgac cctgacagtg tggacaggtc tgctgcacgg
     3601 caaagaaacc ctgacgcatc tgcacctgac cagaaccatg acaggtcgga caggtctgcg
     3661 gctgtgtacc tggttttgca ccgctaccgt ggcaaacgtc acactcttcc agagtcggaa
     3721 tgcggatctc tttggtcacg ccacgtacag cttcttcgag ggtgagctcc atgttatagc
     3781 gtaaatcagc accgcgcgcc gcacgttgac gaccacgtcc gccgccaaaa atatcgccga
     3841 aaacgtcacc aaaaatatcg ctgaagtctg cgccgccgcc aaaaccgccg ccgcccatac
     3901 caccttgctc aaacgcagca tgaccatact gatcgtatgc cgcacgtttt tgcgagtcgg
     3961 tcagaacttc ataagcttcc ttgatctctt taaatttcgc ctcggcctct ttgtcaccct
     4021 ggttacggtc cgggtggtat ttcatggcca ggcgtttgta ggcctttttg atttcacgct
     4081 cttccgctgt tttggaaacg cctaaaatct cgtaataatc ttgcttagcc atctttttta
     4141 aattgcccct agatgaatgc acgggcggag aggaaattcc ccttcgcccg tgtcagtata
     4201 attacccgtt tatagggcga ttattttttg tctttgactt cttcaaattc agcgtcgaca
     4261 acatcgtcat ctttcgcgtt gtttgcagaa gcatcagcac cggcagtctg ctgctgggca
     4321 tgttgctgct gggcgatttc aatcagtttc tgggaaacct gtgccagctc ctgcattttc
     4381 gcttcgatag cggctttgtc ttcacctttc agagcagttt ccagtgcagt cagtgcagac
     4441 tcgatagcag ttttgtcgtc agccggcagt ttgtcgcctg cttcttcaac ctgcttacgg
     4501 gtgctgtgca gcagatggtc gccctggttg cgagcctgta ccagctcttc aaacttacgg
     4561 tcagcttcgg cgttagcttc tgcgtcgcgt accattttct ggatttcatc ttcgttcaga
     4621 ccagaagaag ccttgatggt gatcttctgc tctttaccgc tgtttttatc tttcgcggaa
     4681 acgtgcagga taccgtcagc atcgatatcg aaggtaactt cgatctgcgg catgccgcgc
     4741 ggtgccgggt tgataccatc caggttgaac tgacccagag atttgttatc agccgcacgt
     4801 ttacgttcac cctgcagcac atggatggtt accgcagact ggttgtcttc agcggtagag
     4861 aacacctggc tgtgcttggt cgggatagtg gtgtttttcg cgatcagcgt cgtcatcaca
     4921 ccgcccatgg tttcgatacc cagagacagc ggggtaacgt ccagcagcag tacgtctttc
     4981 acgtcaccag tcagaacacc accctgaaca gcagcaccga ttgctacagc ttcgtccggg
     5041 ttaacgtctt tacgcggctc tttaccaaag aactcagcaa ctttcttctg aaccattggc
     5101 atacgagtct gaccaccaac gaggataacg tcgtcgatat cagatacgga caggccagcg
     5161 tcctgcagtg caactttcag cggctcaatg gaacggttta ccagatcttc aaccaggctt
     5221 tccagtttcg cacgagtcac tttgatgttc atgtgtttcg gaccggtcgc atctgcagtg
     5281 atgtacggca ggttaacgtc ggtctgctga gcggaagaca gttcgatttt cgctttttct
     5341 gccgcttctt tcaggcgctg cattgccagc ggatcgttgc gcaggtcaat gccctgatct
     5401 ttcttgaatt cttcaaccag gtagttgatc agacggctgt cgaagtcttc accacccagg
     5461 tgggtatcac cgttggtagc cagaacttcg aaggtttttt cgccgtcaac ttcgtcgatt
     5521 tcgataatag aaatatcgaa agtaccacca cccaggtcat aaaccgcgat agtacggttg
     5581 ccagtacctt tgtccagacc gtaagccagc gcagctgcgg tcggttcgtt gatgatacgt
     5641 tttacttcca gaccagcgat acggcctgcg tctttggttg cctgacgctg agcatcgtta
     5701 aagtatgccg gtacggtgat aacagcttca gttaccggtt cacccaagta atcttcagcg
     5761 gttttcttca tttttttcag cacttcagca gaaatctgcg gcggtgccat tttctggcct
     5821 ttaacttcga cccatgcgtc gccgttatca gcagcaataa ttttgaacgg catgatggaa
     5881 acatcacgct gtacttcttc gtcctggaag cggcggccaa tcaggcgttt aatcgcaaac
     5941 agggtgtttt gcgggttcgt cactgcctga cgtttagccg gctgaccaac cagagtttca
     6001 ccatcctggg tataggcaat aatagaaggc gtggtgcgat cgccttcggc gttctccagc
     6061 acgcgaggag tggtgccatc cataatcgct acacaagagt tggtagtacc caggtcgata
     6121 ccaattattt tacccatcta aacgtctcca ctatatattc ggtcatcatg tggttgtgag
     6181 tctgtaatag gggcgaaacg tctggtttca actgcccaat ttcatttttt tgcagactca
     6241 ctcactgcgg ttgactacta aatggggtcg taacccacgt catcaagggg gagatgcaaa
     6301 aattttttgt gcttttgcgc agaaatttcg cgatatagca ggacaagacg tgaaactgac
     6361 agcaatttta ttcatataaa gaatgaatcg cagccaagac attcatcaaa aaattgtaat
     6421 aaaaagaaaa cattacgcac ctgaatcttc tctttatcag cagtaaacta gtgggtatcc
     6481 atccccctac ctctccccac taagagaatc cttatgaaat ccgtttttac gatttccgcc
     6541 agcctggcga ttagtctgat gctgtgctgc acggcgcagg caaacgacca taaaatcctc
     6601 ggcgtcattg cgatgccacg taacgaaacc aacgatctgg cgctaaaact tcctgtttgc
     6661 cgaattgtta aacgcataca actctctgcc gaccatggcg atttacagtt aagcggtgca
     6721 tcggtttatt tcaaagccgc ccgtagcgcc agtcagagcc tgaatattcc ttcagaaata
     6781 aaagaagggc aaaccactga ctggatcaac attaacagcg ataacgacaa taaacgctgc
     6841 gtctcaaaaa tcaccttttc cggtcatacg gtgaactcat cggatatggc cacgctgaaa
     6901 attatcggcg acgactaacg ccagaatata gaagccacaa aaaatgaatg ttaattacct
     6961 gaatgattca gatctggatt ttctccagca ttgcagtgag gaacagttgg caaatttcgc
     7021 ccgattgctc acccataatg aaaaaggcaa aactcgcctc tccagcgtac tgatgcgcaa
     7081 cgaactgttt aaatcgatgg aagggcatcc cgagcaacat cgccgcaact ggcagctgat
     7141 tgctggagaa ttacagcatt ttggtggcga tagtatcgcc aacaaactgc gcggacacgg
     7201 taaattgtat cgggccattt tgctcgatgt ttcaaaacga ttgaagctga aagccgacaa
     7261 agagatgtct acgtttgaga ttgagcaaca gttactggaa caatttctgc gtaacacctg
     7321 gaagaatatg gacgaggaac ataaacagga gtttctgcac gcggtcgatg cgagggtgaa
     7381 tgagctggaa gagctgctgc cgctgctgat gaaagacaaa ttattggcaa aaggcgtgtc
     7441 gcatttgctt tccagccaac tgacccgcat tttacgcacc cacgcagcaa tgagcgtact
     7501 tgggcatggt ttgctgcgcg gcgcggggct gggaggccct gtaggtgcgg cactaaatgg
     7561 ggttaaagcg gtcagcggca gcgcctatcg cgtgacgatt ccagccgtac tgcaaatcgc
     7621 ctgcctgcgc cggatggtta gcgccactca ggtctgatac cggaaaccgg ggaaaatttt
     7681 cattttcccc tcaaaagatc gtagacactg ccccactggc tgattattat gccgcgccct
     7741 gaaaacacta cagttattca gggaaattat ttcaccattc attcgatgat gatttttgag
     7801 gaattatggg caacactaag ttggctaatc cggcaccgct gggcctgatg ggcttcggca
     7861 tgaccaccat tctgcttaac ctgcacaacg tgggttattt cgctctggac ggtattattc
     7921 ttgccatggg cattttctac ggcggcatcg cgcaaatttt tgctggtctg ctggagtaca
     7981 aaaaaggcaa cactttcggt ttaaccgcat tcacctctta cggttctttc tggctgacgc
     8041 tggttgcgat tctgctgatg ccgaaactgg gtctgaccga tgcgccaaat gcacagttcc
     8101 ttggtgtcta tctgggtctg tggggcgtat ttacgctgtt tatgttcttc ggcacgctga
     8161 aaggcgcacg cgttctgcaa ttcgttttct ttagcctgac cgtgctgttt gccctgctgg
     8221 cgatcggtaa cattgccggt aacgccgcaa tcatccactt tgccggctgg attgggctga
     8281 tctgcggtgc cagcgcaatc tatctggcga tgggtgaagt actgaacgag cagtttggtc
     8341 gcaccgttct gccgattggt gaatcccact aattgttttg agatcccccc gctcgggggg
     8401 atttttttat tcgctaacgt cgcgtcttgc actcttcggt ctgaatgctg caaccacttc
     8461 cggtgccgtt tcaacgtatg gcccttccag caactgaatg cagtacggta cgctggcaaa
     8521 aataccgtgt accacaacgt tacccgcagc gtccttcaca ccttccagcg tctctttaat
     8581 agacttcggc tgaccgggta agttaaggat cagcgcctgt ttgcgaatca caccaacctg
     8641 acgcgaaagg atcgcagttg gtacaaaatg caggcttatc tggcgcatct gttcaccaaa
     8701 gccaggcatc tcgcggtccg ctactgccag cgtcgcatcg ggcgttacgt cacgacgcgc
     8761 cgggccagtt ccgcccgtgg tgagcaccag atggcaactc atttcatcca ctagctcaca
     8821 caacgtttgc tcgatgatcg cctgctcatc ggggattaag cgggtttcca gttcaaacgg
     8881 cgtggttagc gccgatgtca gccattcttc cagcgcaggg atgcctttat cctgataaac
     8941 gccgctggat gcgcgatcag agatggaaac taagccaata cgtaaagtat tcatcaagat
     9001 ttttccgttt aaacagtctc gttaaacaga atgatacact gcgaagggag tgacagacag
     9061 gcttcagaag aggtagcgtg accgacttcc cggtcacgct aagaatgatt acagcagatc
     9121 gccgatcatt ttttccagtt tttcctggtc aatagcaaac ttacggatac cttccgccag
     9181 tttatctact gccattggat cctggttgtg ctgccacagg aactcggact cagtgatacg
     9241 cgccggacgc gctttcactt cgccggtgta agacagttta cgttcgatag ccccttcgct
     9301 ctcagccagc tctttcagca gtgccggtgc gatggtcaga cggtcgcagc ctgccagttc
     9361 cagaatttcg ccgatgttac ggaagcttgc gcccataacc acggtttcat aaccgtgctc
     9421 tttgtagtac tggtagattt cagatacaga aaccacgccc ggatcttctg ccggagcgta
     9481 ctctttctta tcggtattcg ctttgtacca gtcaagaata cggccaacaa acggcgagat
     9541 caggaacacg cccgcttccg cacaagcacg agcctgagcg aaggagaaca gcagggtcag
     9601 gttacagttg atgccttctt tttccagctg ttctgcagca cggataccct gccaggtaga
     9661 agccagtttg atcagaatac gatcgttgct aataccagca tcgttgtaga gtttgatcag
     9721 gcgttttgct ttcgcaattg acgcttcagt gtcataggaa agacgcgcat caacttcagt
     9781 tgagatacgg cccggaacca gtttcaggat ttccagacca atatttactg ccagtttgtc
     9841 ggtcgcgtcc acgatctgct gcgcgcgatc gttgctctgc tgtttcgccc aggcgacagc
     9901 atcatcaatc aacttacggt attccggaat ctgcgctgcg ttaagaatga gagaagggtt
     9961 ggttgtggca tcctgcggtt gatacagctt cattgccgcg atgtccccag tgtcggccac
    10021 tacggtggtg tactgacgaa gggaggtcaa tttgtccgtc atgatagtat ttctctttaa
    10081 acagcttgtt agggggatgt aaccggtctg ccctgatgat atcacgacgc aacgtcagcg
    10141 caaccgccga caagacggtt atcgccaggc gagactgttt cggatttctg acactgcgtt
    10201 gatatcgccc gccattttta tacaaaacct catgtatgct acgcagaagt tatcaagtac
    10261 ctcgtagcgt atatacttct taaacaattg gtaacgttta cacaggaaag tcatcgcgac
    10321 cggcaataag agggatatgc atgccagatt ttttctcctt tattaacagc gtcctttggg
    10381 gatcggtaat gatttacctg ctcttcggcg caggttgttg gttcactttt cgcaccggat
    10441 ttgtgcagtt tcgctacatc cgccagtttg gcaaaagtct taaaaatagc attcatccac
    10501 agccaggcgg tttaacctca tttcagtcat tgtgtaccag tcttgcggcg cgcgtgggta
    10561 gcggcaacct ggccggcgtt gcgctggcaa ttaccgccgg tggacctgga gccgtctttt
    10621 ggatgtgggt tgccgcgttt atcggcatgg cgacctcgtt tgccgaatgt tctctggcac
    10681 aactttataa agaacgtgac gtcaatggac agtttcgtgg cggaccggca tggtatatgg
    10741 cgcgcgggct ggggatgcgt tggatgggcg ttctgttcgc cgtctttttg ctcatcgcct
    10801 atggcataat tttcagcgga gttcaggcga acgccgttgc ccgcgcccta agtttttctt
    10861 ttgattttcc tccgctggtg acaggcatta ttctcgctgt ctttgctctg ctggcaatca
    10921 ctcgcggtct tcatggcgtc gcccggctca tgcagggttt tgtcccgttg atggcgataa
    10981 tctgggtact gaccagcctg gtgatttgcg taatgaatat cgggcaactt ccccacgtca
    11041 tttggtctat ttttgagagt gcttttggct ggcaggaagc ggcaggcggc gcggcgggat
    11101 ataccttaag ccaggcgatt actaacggtt ttcagcgcag tatgttttcc aatgaggcgg
    11161 gaatgggttc tacaccaaac gcagcagcgg cagcggcgtc ctggcctccg catccggcag
    11221 cgcaagggat tgtccagatg attggcattt ttatcgacac cctggtcatc tgtacggcaa
    11281 gcgccatgct gatattactg gcgggtaacg gcacaaccta catgccgctg gaaggtattc
    11341 agcttatcca gaaggcgatg cgggtgctaa tgggttcctg gggtgctgag tttgttaccc
    11401 tcgtggttat tctgtttgcc ttcagctcca tcgttgccaa ctacatttat gccgaaaaca
    11461 atctcttctt tttacgcctg aacaacccta aagcgatctg gtgtttgcgg atctgcacct
    11521 tcgcaacggt catcggcggc accttgctaa gtcttccgct gatgtggcaa ctggcagata
    11581 tcataatggc ctgcatggct attaccaatt tgaccgccat tttactgctc tcgcctgtgg
    11641 ttcataccat tgccagtgat tatctacgcc agcgtaaact cggcgtgcgc ccggtgtttg
    11701 atccgttgcg ttatccggat atcggtcgcc agctttctcc ggacgcgtgg gatgatgttt
    11761 cgcaggagta atcacaacta tcgatcaact cattctcttt ttttgctaaa gtcggcataa
    11821 atttcctgca aggactggat atgctgattc ttatttcacc tgcgaaaacg cttgattacc
    11881 aaagcccgtt gaccaccatg cgctatacgc tgccggagct gttagacaat tcccagcagt
    11941 tgatccatga agcgcggaaa ctgacgcctc cgcagattag cacgctgatg cgcatcagcg
    12001 acaaactggc gggtatcaac gccgctcgct ttcatgactg gcagccagat ttcacgccgg
    12061 agaatgcccg ccaggcgatt ctggcgttta aaggtgatgt ctacaccggc ttgcaggccg
    12121 aaaccttcag cgaagacgat ttcgattttg cccaacagca tttgcgaatg ctttccggct
    12181 tgtatggcgt actgcgcccg ctcgatttaa tgcagcctta tcgtctggaa atggggatcc
    12241 gtcttgagaa tgcccgaggg aaagatctgt atcaattctg gggagatatc atcaccaaca
    12301 agctgaacga ggcgctcgca gcacaaggcg ataatgtggt gattaatctg gcgtcagatg
    12361 agtattttaa atcggtgaag ccgaagaaat tgaatgccga gattatcaag ccggtgttcc
    12421 tcgatgagaa gaacggcaaa tttaagataa tcagcttcta cgctaagaaa gcacgcggtc
    12481 tgatgagtcg ttttattatt gaaaatcggc tgaccaaacc agagcaactg actggtttta
    12541 atagcgaagg ttacttcttt gatgaagctt cctccagcaa tggcgaactg gtgtttaaac
    12601 gctacgagca gcgttaagtt tgtagtcaat gccggatgcg gcgtgaacgc cttatccggc
    12661 ctacaaaagc atgcagattc aatatattgc agaattcacg taggcctgat aagacgcgcc
    12721 agcgtcgcat caggcgctga atgccggatt gttacccggc atttgtcatt tagcggtgat
    12781 gtttgcctgg accatgaccg ccgtgatgat catgaggagc tttcttatgg tggcgcggcg
    12841 gtggcggcgg tccgtgtggg tgccagcgat tgcctcgcca ttcataatgt tgtttccacc
    12901 agccgtggtc gcgccagtga cctccatccc agtaatagcc acgattatca cgatcgccta
    12961 tctgtaattt tactgacggg actaacgtaa tttccgcagc ctgtgctgcc atgggagcga
    13021 ccagaaccag ggaaagtgcg agtacgatag attgcatctt tttcacaggt tactcctttt
    13081 atcattgccc gttatgggtc gatatccgga gattacgcga tgagaacgac ggttgttatt
    13141 ctccgcaatc ctaatctcta agttaccgca tttattgaga atttctcctt tttccctgtc
    13201 gtttttctcc ataatttctt cataaaaaag ccgggctgca taaaagcaaa cccggcctga
    13261 ttgagataat gaatagattt tactgatgat tcatcatcaa tttacgcaac gcagcaaaat
    13321 cggcgggcag attatgtgaa agcaagggta aatcagcacg ttctgccagc tcttttggca
    13381 gatccaacgt ttcaccgaga atcgcttcca cgctctcttt aaatttcgcc ggatgcgcgg
    13441 tgccgaggaa caagccatat tcgcctggat tcaactggtc acgcagcgca cgatacgcta
    13501 cggcagcgtg cggctccgag gtgtagccca gttcttttaa ctcacgcatt gtctgttgcg
    13561 tggtttcatc atccacggct gcataaccca gctctttcag ttgccagatt ttgcggcgga
    13621 acaactcttc cacacgcggc cagttgttcg gctgactcac gtccatcgcg ttggataacg
    13681 tcgcctgagt cgctttgggt gaccactgac cgtcgtgcag gaaacgtggc acggtatcgt
    13741 tcacgttggt cgcagcaata aaacgtttca ccggcagacc gagtgacttc gccagcagac
    13801 ccgccgtcaa atcgccgaag tttccgcttg gcaccgagac aaccagctgg ttgcgcgctt
    13861 cctgcggcag ctgcgcaaca gcttcaaagt agtagcaaat ctgcgccagc aaacggctga
    13921 tgttaatcga gttagctgag tttaacccca gcgccacttt cagttcttca tcatcaaacg
    13981 cctgcttcac cagcgcctga caggcatcga aatcgccgtc gatggcaaca gtttcgatat
    14041 tgccgcccaa cgtacagaac agtttttctt gcagtggact gattttgcct cgtggataga
    14101 ggataaccac tttcacattc ggtaaaccgt agaaagcatg agccactgcc gctccggtat
    14161 caccggaggt cgcggtcaga atggtcactg gcttatcgcc cgcaatatgg gtcagcattt
    14221 gtgccataaa gcgaccgccg aaatctttaa atgccagcgt tggcccgtgg aacaattcca
    14281 gacaaccgac atcgctttca acattggcga ccggagccgg gaaggcaaac gccgcgcgca
    14341 cgcgctcttc caggatttcc tgcgggattt catcaccaat aaacgccgag aggatcttcg
    14401 cactgcgggt gacaaaatcc agcttcagca tctcatcaat ttcagtcagg ctgaattccg
    14461 gcaggtcatg cggaaaaaac agcccctgat ttttgcccaa cccctgggtt acggcttgcg
    14521 caaagctgac ctgctcgttg tgatctttca gattgtagag tttcatttag ttttccagta
    14581 ctcgtgcgcc cgccgtatcc agccggcaaa tatgaacaaa accttcctga ttttgcaggt
    14641 agttcttacc caaccagtcg gcaacgcgct gggcggtatc cggcttgtca catagagcga
    14701 acaaggtcgg gccggagccg gagataccgc tcgctaccgc gccgatttcc gcgaccgcct
    14761 gccgcgcctg ccggaagcca ggcagtaacc gttcacggta gggttcagcg ataacatctt
    14821 tcatcagctt cgcggcaagc tcaggctgac gggaatagca ggcgtgaatg aagccagcca
    14881 gatgtcgccc gtgcgcaatg caatcctggc ggcgatactg cgccggtaaa atagccctgg
    14941 cttctgccgt cgagacttta atccccggat acgccagcac ccacagccac tcatcaaacc
    15001 ctggcacttg ctggctgatg atgtcgtttt cttcgatcat caactgcata ccaccaagaa
    15061 aacacggtgc cacgttgtcg taatgaatgc tgccggagat tcgtccttcc agctcgccca
    15121 tcaaagccag caaacgagtg tcattaagcg gcttgccaca gtgttcattc atcgccatca
    15181 gcgccgcgac caccgaacag gcgctggagc ctaagcccga accgattggc atattctttt
    15241 ccagagtcat cgccactgga atttgcttgc caagctcctg gcaaaaacgc tcccagcact
    15301 gataaacgat attttcccgt ggttctgacg gcagcttatc ggcaaagcgt ccgaggttgt
    15361 tgagactgaa tgtctctgcc gcctcaaccg tgactacatc tccgagcaat gcaccatcaa
    15421 caggtgtcac cgccgccccg agcacatcaa acccgacgct catattggca ctggaagccg
    15481 gggcataaac tttaaccatg tcagactcct aacttccatg agagggtacg tagcagatcg
    15541 gcaaagacac cggcagctgt aacgtcattg cccgcaccat atccgcgcag caccaacggc
    15601 agcggctgat aatagtggct ataaaaggcc agggcgtttt cgccattttt cactttgaac
    15661 agcggatcat taccatccac ttcggcaatc ttcacgcggc aggcaccatc ttcatcaata
    15721 ttgccaacat agcgcaaaac ttttccttca tcacgggcct tcgccacgcg cgcggcaaag
    15781 agatcgtcga gctgtgacag attcgccata aaagcggcaa catcaccctc agcgttaaac
    15841 tctgcgggca gcacaggttc aatttcaata tccgccagct ccagttcacg tcccgtttca
    15901 cgagcgagaa tcaatagctt acgcgctaca tccataccag aaagatcatc tcgcggatcc
    15961 ggttcggtat aacccatttc ccgcgccaga gtagtcgcct cggagaaact catgccttcg
    16021 tctaacttgc cgaagatata agaaagcgaa cctgaaagaa tgccggagaa cttcatcaat
    16081 tcatcaccag cattgagcag attttgcagg ttctcaataa ccggtaatcc agccccaacg
    16141 ttggtgtcat agaggaattt acgccgcgat ttttccgccg catgacgcaa cagatggtag
    16201 taatccatcg acgaggtgtt ggcctttttg ttcggcgtga caacgtggaa accttcgcgc
    16261 aagaagtcgg catattgatc cgccactgcc tggctggagg tgcagtcaac aatgaccggg
    16321 ttcagcagat gatattcttt cacgaggcga attaagcgcc cgagattaaa cggctctttg
    16381 gcttgcgcca gttcttcctg ccagttttcc agatttaggc catgcacatt ggtgagcagt
    16441 gccttcgagt tggcaacacc gcagacacgt aagtcgatat gtttattctt cagccagctt
    16501 tgttgacgct tcagttgctc cagcagcgca ccgccaacgc caccgacgcc aatcacaaac
    16561 acttcgataa cctgatcggt attgaacagc atctgatgag taacgcgcac gccagtggtc
    16621 gcatcatcgt tatttaccac gacagagatt gagcgttcag aagatccctg agcaatggcg
    16681 acaatgttga tattggcgcg ggccagcgcg gcaaagaatt tcgccgagat cccacgcaag
    16741 gtgcgcatac catcacctac caccgagata atggccagcc gttccgtcac cgccagcggc
    16801 tccagtaagc cttctttcag ttccaggtag aactcttcct gcattgcccg ttcagcccgc
    16861 acacagtcgc tttgcggaac gcagaaactg atactgtatt cggaagatga ttgcgtaatc
    16921 agcaccacgg aaatacgggc gcgtgacatc gcggcaaaga cgcgcgccgc catgccgacc
    16981 atccctttca ttcccggacc agaaacgctg aacattgcca tgttattcag attggaaatg
    17041 cccttgaccg gtaattcgtc ttcatcacgg ctggcaccaa tgagcgtacc tggtgcttga
    17101 ggatttccgg tatttttaat caggcaaggg atctggaact gggcgatggg ggtaatggtg
    17161 cgggggtgaa gaactttagc gccgaagtag gaaagctcca tcgcttcctg gtaggacatc
    17221 gacttcaaca acctcgcatc gggcacctga cgcgggtcgc aggtatagac cccgtcaacg
    17281 tccgtccaaa tctcgcaaca atcggcgcgt aaacaggcag ccagcaccgc cgcggagtag
    17341 tcggaaccgt tgcgtccaag taccaccagt tcgccttttt cattaccggc ggtgaagcct
    17401 gccatcagca ccatgtgatc agccggaata cgacttgccg caatacggcg ggtggactca
    17461 gcaatatcga cggtagattc gaggtaatgc cccactgcca gtagtttttc gaccggatcg
    17521 ataacggtaa cgttgtgacc acgcgcttct aacacgccgg ccataatggc gatcgacatt
    17581 ttctcgccac ggcaaatcag cgcagcgttg atgctatccg ggcactgccc caacaaacta
    17641 atgccatgca ggacatgttt tatttgggca aattcctggt cgacgaaaga tttcagttgc
    17701 gccagcggga atcccggctg ggcggcggtg agtcccgtca gaagttcggc aaaaatacgt
    17761 tcggcatcgc tgatattggg taaagcatcc tggccgctaa tggttttttc aatcatcgcc
    17821 accaggtggt tggtgatttt ggcgggggca gagaggacgg tggccacctg cccctgcctg
    17881 gcattgcttt ccagaatatc ggcaacccgc agaaaacgtt ctgcatttgc cactgatgta
    17941 ccgccgaact tcaacactcg catggttgtt acctcgttac ctttggtcga aaaaaaagcc
    18001 cgcactgtca ggtgcgggct tttttctgtg tttcctgtac gcgtcagccc gcaccgttac
    18061 ctgtggtaat ggtgatggtg gtggtaatgg tggtgctaat gcgtttcatg gatgttgtgt
    18121 actctgtaat ttttatctgt ctgtgcgcta tgcctatatt ggttaaagta tttagtgacc
    18181 taagtcaata aaattttaat ttactcacgg caggtaacca gttcagaagc tgctatcaga
    18241 cactcttttt ttaatccaca cagagacata ttgcccgttg cagtcagaat gaaaagctga
    18301 aaaatactta ctaaggcgtt ttttatttgg tgatattttt ttcaatatca tgcagcaaac
    18361 ggtgcaacat tgccgtgtct cgttgctcta aaagccccag gcgttgttgt aaccagtcga
    18421 ccagttttat gtcatctgcc actgccagag tcgtcagcaa tgccatggct cgttcgcgta
    18481 aagcttgcag ttgatgttgg tctgccgttg tgtcactttt cgccggttgt tgtattaatg
    18541 ttgctaattg atagcaatag accatcaccg cctgccccag attgagcgaa ggataatctg
    18601 ccaccatcgg cacaccagta agaacgtcag ccaacgctaa ctcttcgtta gtcagcccgg
    18661 aatcttcgcg accaaacacc agcgcggcat ggctcatcca tgaagatttt tcctctaaca
    18721 gcggtaccag ttctactggc gtagcgtagt aatgatattt cgcccgactg cgcgcagtgg
    18781 tggcgacagt gaaatcgaca tcgtgtaacg attcagccag tgtcgggaaa actttaatat
    18841 tatcaataac atcaccagat ccatgtgcga cccagcgggt ggctggctcc aggtgtgcct
    18901 gactatcgac aatccgcagt tcgctaaaac ccatcgtttt cattgcccgc gccgctgccc
    18961 caatattttc tgctctggcg ggtgcgacca gaataatcgt tatacgcata ttgccactct
    19021 tcttgaccaa ataaccgcga acagggtgat cactgtcaac ttattacgcg gtgcgaattt
    19081 acaaatcctt aacgtaagtc gcagaaaaag ccctttactt agcttaaaaa aggctaaact
    19141 atttcctgac tgtactaacg gttgaattgt taaaaaatgc tacatatcct tctgtttact
    19201 taggataatt ttataaaaaa taaatctcga caattggatt caccacgttt attagttgta
    19261 tgatgcaact agttggatta ttaaaataat gtgacgaaag ctagcattta gatacgatga
    19321 tttcatcaaa ctgttaacgt gctacaattg aacttgatat atgtcaacga agcgtagttt
    19381 tattgggtgt ccggcccctc ttagcctgtt atgttgctgt taaaatggtt aggatgacag
    19441 ccgtttttga cactgtcggg tcctgaggga aagtacccac gaccaagcta atgatgttgt
    19501 tgacgttgat ggaaagtgca tcaagaacgc aattacgtac tttagtcatg ttacgccgat
    19561 catgttaatt tgcagcatgc atcaggcagg tcagggactt ttgtacttcc tgtttcgatt
    19621 tagttggcaa tttaggtagc aaacatgcag accccgcaca ttcttatcgt tgaagacgag
    19681 ttggtaacac gcaacacgtt gaaaagtatt ttcgaagcgg aaggctatga tgttttcgaa
    19741 gcgacagatg gcgcggaaat gcatcagatc ctctctgaat atgacatcaa cctggtgatc
    19801 atggatatca atctgccggg taagaacggt cttctgttag cgcgtgaact gcgcgagcag
    19861 gcgaatgttg cgttgatgtt cctgactggc cgtgacaacg aagtcgataa aattctcggc
    19921 ctcgaaatcg gtgcagatga ctacatcacc aaaccgttca acccgcgtga actgactatt
    19981 cgtgcacgca acctgctgtc ccgtaccatg aatctgggta ctgtcagcga agaacgtcgt
    20041 agcgttgaaa gctacaagtt caatggttgg gaactggaca tcaacagccg ttcgttgatc
    20101 ggccctgatg gcgagcagta caagctgccg cgcagcgagt tccgcgccat gcttcacttc
    20161 tgtgaaaacc caggcaaaat tcagtcccgt gctgaactgc tgaagaaaat gaccggccgt
    20221 gagctgaaac cgcacgaccg tactgtagac gtgacgatcc gccgtattcg taaacatttc
    20281 gaatctacgc cggatacgcc ggaaatcatc gccaccattc acggtgaagg ttatcgcttc
    20341 tgcggtgatc tggaagatta atcggcttta ccaccgtcaa aaaaacggcg ctttttagcg
    20401 ccgtttttat ttttcaacct tatttccaga tacgtaattc atcgtccgtt gtaacttctt
    20461 tactggcttt cattttcggc agtgaaaacg cataccagtc gatattacgg gtcacaaaca
    20521 tcatgccggc cagcgccacc accagcacac tggttcccaa caacagcgcg ctatcggcag
    20581 agttgagcag tccccacatc acaccatcca gcaacaacag cgcgagggta aacaacatgc
    20641 tgttgcgcca gcctttcaat accgcctgca aataaatacc gttcattaac gccccaatca
    20701 gactggcgat tatccatgcc acggtaaaac cggtatgttc agaaagcgcc agcaagagca
    20761 aataaaacat caccaatgaa agccccacca gcaaatattg cattgggtgt aagcgttgcg
    20821 cggtgagcgt ttcaaaaaca aagaacgcca taaaagtcag cgcaatcagc agaatggcat
    20881 acttagtcgc ccggtcagtt aattggtatt gatcggctgg cgtcgtcact gcgacgctaa
    20941 acgcggggaa gttttcccag ccggtatcat tgcctgaagc aaaacgctca ccgagattat
    21001 ttgcaaacca gctgctttgc cattgcgcct gaaaacctga ctcgctaact tcccgtttgg
    21061 ctggcaaaaa atcacctaaa aaactgggat gcggccagtt gctggttaag gtcatttcgc
    21121 tattacgccc ggcaggcacc acagaaagat cgccggtacc gcttaaattc agggccatat
    21181 tcagcttcag gttctgcttg cgccagtccc cttcgggtaa agggatatgc acgccctgcc
    21241 cgccttgctc taacccggta ccgggttcga tggtcagcgc tgttccgtta acttcaggcg
    21301 ctttcaccac accaatacca cgcgcatccc cgacgctaat cacaataaat ggcttgccta
    21361 aggtgatatt tggtgcgtcg agttcgctaa gacgcgaaac atcgaaatcg gcttttagcg
    21421 ttaaatcact gtgccagacc tgaccggtat aaatccctat cttgcgttct tcaacgttct
    21481 gattgccatc aaccatcaat gactcaggta accaaaaatg gataaaactt cgtttccgct
    21541 ccacggtttt atcgtcttcc tgcaccgtat aaagctcggt cacaggaata gcgatgagcg
    21601 gcccaacgag tttttgcggc ccgctggtac tttggcgaat cgcatcttcc acatcgctac
    21661 ggtaatcggc acgttcgaca atcacctgcc gaatcagcat tatcggaatc aacaacagca
    21721 atactgcacc aaacaaggtg gtcattttcc agaacagggg ggatttcaac atagtcttct
    21781 cctttgcaat ggcagcagga tacgaa
//
LOCUS       DAMZET010000068        21235 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.068, whole genome shotgun sequence.
ACCESSION   DAMZET010000068 DAMZET010000000
VERSION     DAMZET010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.068"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..309
                     /locus_tag="POB34_002572"
     CDS             <1..309
                     /locus_tag="POB34_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein StaE"
                     /protein_id="HDI5919495.1"
                     /translation="TKRATCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSA
                     TQFSCVTPASQEVTISNAANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN
                     "
     gene            336..932
                     /locus_tag="POB34_002573"
     CDS             336..932
                     /locus_tag="POB34_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI5919496.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            985..2259
                     /locus_tag="POB34_002574"
     CDS             985..2259
                     /locus_tag="POB34_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HDI5919497.1"
                     /translation="MKKFFRHFLFLILCLSCYTASAGTDDNVGYIVGNSYGVGPSDQK
                     WRETGPNGDATVIFRYATSTNNLVFYKPTQLGPTGVKLQWSQLDTASGGGFLYCNRSD
                     STSGSAMRIENAMVDSGKMYGSHKLFNTSVPGLYYTLLISNMWSAYGTVTNVSSPGIY
                     IGDSAEQYFSWYNPSEDVLYWSCNNANSTRKYWAVGGIYQTLTIEFYTDTNFDPTVTQ
                     QIKLSSSSNYLYSFKAYGAGQGINEHSYFIKIDFDLLNVKLTNPTCFTAMLSGTSVTG
                     STVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIETKLVSTKVGTENGQLLGNTLTG
                     NDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYDPRGKDDTTGGVYPDQDTGITY
                     PLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            2372..3166
                     /gene="panB"
                     /locus_tag="POB34_002575"
     CDS             2372..3166
                     /gene="panB"
                     /locus_tag="POB34_002575"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDI5919498.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITDALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            3178..4029
                     /gene="panC"
                     /locus_tag="POB34_002576"
     CDS             3178..4029
                     /gene="panC"
                     /locus_tag="POB34_002576"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDI5919499.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(4103..5005)
                     /gene="rpnC"
                     /locus_tag="POB34_002577"
     CDS             complement(4103..5005)
                     /gene="rpnC"
                     /locus_tag="POB34_002577"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HDI5919500.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            5279..5659
                     /gene="panD"
                     /locus_tag="POB34_002578"
     CDS             5279..5659
                     /gene="panD"
                     /locus_tag="POB34_002578"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDI5919501.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(5663..6892)
                     /locus_tag="POB34_002579"
     CDS             complement(5663..6892)
                     /locus_tag="POB34_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDI5919502.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDMPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(6956..7396)
                     /locus_tag="POB34_002580"
     CDS             complement(6956..7396)
                     /locus_tag="POB34_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDI5919503.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(7501..8271)
                     /locus_tag="POB34_002581"
     CDS             complement(7501..8271)
                     /locus_tag="POB34_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5919504.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(8268..9194)
                     /locus_tag="POB34_002582"
     CDS             complement(8268..9194)
                     /locus_tag="POB34_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5919505.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            9303..9965
                     /gene="can"
                     /locus_tag="POB34_002583"
     CDS             9303..9965
                     /gene="can"
                     /locus_tag="POB34_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HDI5919506.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(10006..10542)
                     /gene="hpt"
                     /locus_tag="POB34_002584"
     CDS             complement(10006..10542)
                     /gene="hpt"
                     /locus_tag="POB34_002584"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDI5919507.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            10748..13138
                     /gene="gcd"
                     /locus_tag="POB34_002585"
     CDS             10748..13138
                     /gene="gcd"
                     /locus_tag="POB34_002585"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HDI5919508.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWLSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKNPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(13340..14890)
                     /gene="cueO"
                     /locus_tag="POB34_002586"
     CDS             complement(13340..14890)
                     /gene="cueO"
                     /locus_tag="POB34_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HDI5919509.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQHGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            15056..15403
                     /locus_tag="POB34_002587"
     CDS             15056..15403
                     /locus_tag="POB34_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HDI5919510.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            15509..16375
                     /gene="speE"
                     /locus_tag="POB34_002588"
     CDS             15509..16375
                     /gene="speE"
                     /locus_tag="POB34_002588"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDI5919511.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLTHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            16391..17185
                     /gene="speD"
                     /locus_tag="POB34_002589"
     CDS             16391..17185
                     /gene="speD"
                     /locus_tag="POB34_002589"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDI5919512.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(17223..17585)
                     /locus_tag="POB34_002590"
     CDS             complement(17223..17585)
                     /locus_tag="POB34_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HDI5919513.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(17760..20357)
                     /gene="acnB"
                     /locus_tag="POB34_002591"
     CDS             complement(17760..20357)
                     /gene="acnB"
                     /locus_tag="POB34_002591"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HDI5919514.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            20712..>21235
                     /locus_tag="POB34_002592"
     CDS             20712..>21235
                     /locus_tag="POB34_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HDI5919515.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSV"
BASE COUNT         5326 a         5597 c         5146 g         5166 t
ORIGIN      
        1 accaagcgag ccacatgcga aggtactgct aatgacggtg cggggttcgc aaatggatcc
       61 acagccgcag ataaagcaag tgccgtcgcc gttgaagtct ggagcaccga aactccggca
      121 acaggtagcg caacacaatt tagttgtgtg acaccagcat ctcaagaggt cactatctcc
      181 aatgcagcca acgcggttgt ttattatccg atgagtgctc gactggtcgt cgaaaaaaat
      241 aaaaccgtaa gcaatgtcac tgcgggtaaa ttttctgcac ctgccacatt tacagtaacc
      301 tataactaac agactgacag atccaggaac tatatatgca tcccactcaa cgtaagctga
      361 tgaagagaat aattctgttt ctgtcactac agtttcccat tacctatcca gccatcgctg
      421 ggcaggatat tgaccttgtt gccaatgtaa aaaacagcac ttgcaaaagc ggaatcagta
      481 accagggtaa tattgatctt ggcgtcgttg gggtgggata tttttcagat aatgttactc
      541 ctgaaagcta tcaaccaggt ggaaaagagt tcactgtcac agtatcagac tgtgcattac
      601 agggaactgg cgatgtgctt aatcagttac atattgattt tagagccctt agcggtgtca
      661 tggctgcagg ttcaaggcaa atatttgcca atgaagtcgc aaccggggct aaaaatgtcg
      721 gtgtcgttct cttttccatt caggacccga ccaatatatt caatgttatt agttcagcag
      781 gaaattctcg ttccgtgtat ccagtaatga cttctgccct gcataattct tcatggaaat
      841 tttatgccag aatgcaaaaa atcgatcccg cgctggatgt catatctggc caggtaatga
      901 gtcatatatt agttgatgtg tactacgaat aatagatcaa aacaaataac tgcgactgtt
      961 acagcagtag atggtggagt gataatgaag aaattcttca gacattttct ttttttaatt
     1021 ctgtgcttga gttgttatac cgcgagtgct ggcactgatg ataacgtagg atatattgtt
     1081 gggaatagtt acggcgttgg tccgtcagat caaaaatgga gagaaacggg gccgaatggc
     1141 gatgctactg tcatattcag atatgcaaca agtacaaata atttagtttt ctacaaaccg
     1201 acgcagcttg gacctacagg tgtaaaatta cagtggagtc agttagatac cgcttctggt
     1261 ggtggttttc tttattgcaa cagaagtgat agcacaagtg gtagcgcaat gcgtattgaa
     1321 aatgcaatgg ttgactcagg taaaatgtat ggctcccata aattatttaa tacatcagtt
     1381 cctggtttgt attacacatt attaatttca aacatgtggt cagcttacgg taccgtaact
     1441 aacgttagtt cacctgggat atacattggt gactctgcag aacaatattt ttcgtggtat
     1501 aatccaagcg aagacgtgtt atattggagt tgcaataatg cgaatagcac ccgtaaatac
     1561 tgggctgtag gtggtattta tcagaccctt acaattgaat tctatacaga tacaaacttt
     1621 gatccaacag taactcaaca gatcaaatta tccagctcat caaattatct gtattcattt
     1681 aaagcctatg gcgcaggcca aggtataaat gagcatagtt attttatcaa aatcgatttc
     1741 gatctgctca atgtcaagtt gactaatccc acttgtttta ccgctatgct tagtggaact
     1801 tctgtgactg gttctacggt aaaaatgggt gaatatagtg cagaacaaat cagaaacggt
     1861 gccacaccgg ttccttttga tatttcactt caaaactgcg ttcgtgtgac taatattgag
     1921 acaaaattag tttcaacaaa ggttggtact gaaaacgggc aactccttgg taatactctc
     1981 acaggtaatg acgcagcaaa aggagtcggt gtactcatag aaggtttagc aactagtaaa
     2041 aatcctctaa tgacattgaa acctaatgat tcaaattctg tttataaaga ttacgacccg
     2101 agaggcaaag acgatacaac aggaggagtt tacccggatc aagatacagg tataacatac
     2161 cctctccatt tccaggccac gctacaacag gatggaacta taccaataga agctggtgag
     2221 tttaaagcca ccagtacttt ccaggtaacc tacccttaat aagtccaccg caccgccatc
     2281 atctggcggt gcagtaattg ataaatcatt gcccccacca atgtgactga taaaatgcca
     2341 ggcaacacga catcatttat caggatacgt tatgaaacca accaccattg cttcacttca
     2401 gaaatgtaag caggataaaa agcgtttcgc gaccatcacc gcttacgact acagcttcgc
     2461 gaaactattt gctgatgaag gacttaacgt catgctggta ggcgactcgc tgggcatgac
     2521 ggttcagggt cacgactcca ccctgccggt gaccgttgct gatatcgcct accacactgc
     2581 cgccgtacgt cgcggcgcgc caaactgcct gctgctggct gatctgccgt ttatggcgta
     2641 tgccacgccg gaacaagcct ttgaaaacgc cgcaacggtt atgcgtgccg gtgccaatat
     2701 ggtcaaaatt gagggcggtg agtggctggt cgaaaccgta aaaatgctga ccgaacgtgc
     2761 cgttcctgta tgtggtcact taggtttaac cccacagtca gtgaatattt tcggtggcta
     2821 caaagttcag gggcgcggcg atgaagcggg cgatcaactg ctcagcgatg cattagcctt
     2881 agaagccgct ggggcacagc tgctggtgct ggaatgcgtg ccggttgaac tggcaaaacg
     2941 tattaccgat gcactggcga tcccggttat tggcattggc gcaggcaacg tcactgacgg
     3001 gcagatcctc gtgatgcacg acgccttcgg cattaccggc ggtcacattc ctaaattcgc
     3061 taaaaatttc ctcgccgaaa cgggcgacat ccgcgcggct gtgcggcagt atatggctga
     3121 agtggagtcc ggcgtttatc cgggcgaaga acacagtttc cattaaggag tcacgttgtg
     3181 ttaattatcg aaaccctgcc gctgctgcgt cagcaaattc gccgcctgcg tatggaaggc
     3241 aagcgcgtgg cgctggtgcc tactatgggt aatctgcacg atggccatat gaagctggtc
     3301 gacgaagcca aagcccgcgc cgatgtggtc gtcgtcagta ttttcgttaa cccaatgcag
     3361 tttgaccgcc cggaagatct ggctcgttat ccacggacct tgcaagagga ctgcgaaaag
     3421 ctaaacaaac gtaaagtgga tttagttttc gccccttcgg taaaagagat ctacccgaac
     3481 ggtactgaaa cccacactta cgttgacgtt cctggccttt cgaccatgct ggaaggtgcc
     3541 agccgtccgg gacattttcg cggcgtttcg actattgtca gcaagctgtt caacctggtc
     3601 cagccggaca tcgcctgctt cggtgaaaag gactttcagc aactggcgct aatccgcaaa
     3661 atggttgccg atatgggctt cgatattgaa attgtcggcg tcccgattat gcgcgccaaa
     3721 gatggccttg cgctcagttc ccgtaatggt tatctgacgg cggaacaacg caaaattgcg
     3781 cccggtctgt acaaagtttt aagttcaatt gccgacaaat tgcaggctgg ggaacgggat
     3841 ctcgatgaaa ttattaccat tgcggggcaa gaactgaatg aaaaaggctt ccgcgccgat
     3901 gatattcaga ttcgcgatgc cgacacattg ctggaagttt ctgaaaccag caaacgggca
     3961 gtaattctgg tagccgcctg gcttggcgat gctcgcctga tcgacaacaa aatggtcgag
     4021 ctggcgtaat acttaactgg cgctacggct gatggcgcca gttattaatt taccccacgt
     4081 catctgtcat aacacaggta acttaaataa ggttaattac cttatcaatc tcagcaagag
     4141 gtaaatttgc catctctgca acgtcttccc gagacattcc tttacttaga agacgcaggg
     4201 cgaattcttg cctttcctcc tgtcttccct gctgaatccc cttctcaatc cctttctctt
     4261 caaaccactg cgccagcgtc atcatagact cccctcccgt ttccctgtct ctcagcacac
     4321 cataaaacaa atccgcttgt tcagtatgac cgcgttgcag catatagttt tgcatggcaa
     4381 ctaactgact tccgctagtg tacccttcgt caatcagcgt gaccagttgc tccagcaata
     4441 acattaagtc gcgctggcga atatgttttt gcagtagttc gagaatcgca atccgccgat
     4501 gttgcatgat ttcgtcatcc ggtgtgatgg tgatatccac cagcgggaaa ggactgttat
     4561 agacgcgtcg tgccagctcc ggcgagtaaa atatatcaaa ccagcacatt gagagcggat
     4621 aaggtgtggc ctcgccctga taaaacagta tcggcaccac cagcggcagc ttatcgtgat
     4681 ccgcctccag atgccggtgc atggcagcaa tagaataacg catcatgcga aaggccattt
     4741 tcttatccgg cttgctttgg tgttcaatca caacatgcag ataaccggga ttgccctgca
     4801 tttgcacgga atagagcacg tccgtgctgt gtcctttcag gctctcttca atgaaactcc
     4861 ccgactctaa atgaagcgtg ttgaggtcac aaagttcgcg taattccact ggcaaatgta
     4921 tctccagaaa gtcgcgagcc gtctccgcat gcattaaaaa ttgtttaaat accgcgtcat
     4981 gcggtgtggt actcggtgca tccatcttcc ctgtcttccg tgttgtcttg cgtgaaggca
     5041 gtatccagtc aaggcgcgcc gtcgtcagcc gcctctttag tgtttaccga gcagcgttca
     5101 gagaaatttc aattcgttac aggcgataca tggcacgctt cggcgcggta cgcaaactgc
     5161 tgacgcaaat gccatcagat gagtagacac tcatcaaaaa tcgggcaata ctgcgtgaga
     5221 atttcctcaa agcaggcacc gcctgcttcg ttaacgacag ggtagaaagg tagaagttat
     5281 gattcgcacg atgctgcagg gcaaactcca ccgcgtgaaa gtgactcatg cggacctgca
     5341 ctatgaaggt tcttgcgcca ttgaccagga ttttcttgac gcagccggta ttctcgaaaa
     5401 cgaagccatc gatatctgga atgtcaccaa cggcaagcgt ttctccactt atgccatcgc
     5461 ggcagaacgc ggttcgagaa ttatttctgt taacggtgcg gcggcccact gcgccagcgt
     5521 cggcgatatt gtcatcatcg ccagcttcgt taccatgcca gatgaagaag ctcgcacctg
     5581 gcgacccaat gtcgcctatt ttgaaggcga caatgaaatg aaacgtaccg cgaaagcgat
     5641 tccggtacag gttgcttgat tgttatccct gcggctggtt actcaccagc cgcgacatcg
     5701 tctccagcga atccgttctt aagatataaa gtcgttttag taacaacgga ttatcccccg
     5761 gttttacttt gcctttcatg gttgtcaccg ccaggtgaaa tccggcatcg tttgctgcct
     5821 tcacggcttt gtcattaaat ccgccaaacg gatacgaaag ataccagaca tgcggattaa
     5881 attgcgccag agcgcggcgt gaacgtgcaa aatcaaacag aatattgtgc tcactacggc
     5941 tcagtaatat gggtcggcga tagccatcca cccgatgcaa aaaatgggta tgtgactgaa
     6001 aatcaaaaac atcgcgaatt tcgttaagct cagaaacgct cataaattgc agcgattttg
     6061 ggttccactt ctgcgggtga cgtttgatgc gcgaggtaac aacaaacgcc gtcgccttca
     6121 tgccatattg tttcaataca ggatacgcat agcggctcac cgacttgagg ccatcatcaa
     6181 aggtaatcac caccgctcgc gcagggagat tgatcttatt cttcacgtaa ccttccagct
     6241 gcgccatgct cagtgtcgcg tatcccctgt cgcgcagcca ggccatctgg ttattgaaag
     6301 cgcgtaccga ggtggtcgtc gaagtatggc gaaaacgggt gttttcttca tcgcgcagaa
     6361 tatggtgata ggttagcacc ggcaggccat tatcgggttg ggcatccagc gcgctgatat
     6421 aggcgagtcg atcgccaata cggatctgat accaggtttg atttaacctg tctttcagtt
     6481 tatgcaaaat cgggtagcgc aaattgtccg ccagtacccc aaatggcgca ctgcccgcac
     6541 tcggcgcgtt atagaccggc atatctttcc aggtaattaa gttctgatta ctcagcggct
     6601 tgttgaggtc acccaaaccg tcttcaactt tttgtcgccc ctgaactggc tcgagatgcc
     6661 ctttatcgat aaacccttta ccaaagccaa aattaaatgc gtaataactt gcggcagtgg
     6721 gctccacggc aatgatttgc ccggcccgaa tattccccac ggtcaccatc ttgtcgccaa
     6781 tttgcgccca gatcgcagca ttttcgatgg tttgcatata acgggcaggt aacgcggcac
     6841 tgacacttgc ggtaaacagc atcagcagga gaataacagc ttgtttatac atgaaaaaat
     6901 aaccgagggt gaaacaacgc ttttattgta gcaaaagcgc caccagaatc gcgggttaat
     6961 actcatacaa attatgtttg aggacgaaag gcggattttt ttgttgttgg tgccaaagac
     7021 tactcacctc cggcgcaccc agttcgacaa tacgctcgcg aaactctgaa ctggtcatgg
     7081 tttcacggca agccatcatc tgttcaatta acggtaacgt gacaccagaa atcacttcgc
     7141 aacgggagtg tttgtggctt aataatgaag ccacgcggta cggtggcgct ccggctatat
     7201 cggttaagaa gatgacaccc tcaccagagt ccgcttcatg cagagcatcg cacatcatgc
     7261 ggctgagcat attagagctt aacccacgcc agaaattcac ggcccggcac tgaagaagcg
     7321 ccccatgttt tttctccagc gcatccagta tctcttgcgc ccggtcatcg tgacaggtaa
     7381 ttacccaacc taacatcgcc ttcctcctta gccgaaaatc agtctaccct gaccattgtt
     7441 gataaagggt gatagccgtc aaaactcctc tcccccaaat ccagaggaga ggaaatagcc
     7501 ttagctacgc aaaccacgtc cacgttggat cagcgaccaa cagatcaaat aaaacgccac
     7561 aataaagacc accagtacgc caaaggtagt gaccagcgga acatcattga taccgaggaa
     7621 gccgtagcgg aaaccactga tcatataaac gattgggttc aggtgcgaca gcccttgcca
     7681 gaacggcggc aacaaagtca gtgagtaaaa gaccccaccc aaatacgtga gtggcgttaa
     7741 cacaaaggtt ggcaccaggc tgatgtcatc gaacgttttg gcaaacacac cgttcagcaa
     7801 acccgcaagg gagaacaaca ccgccgtgag caccagggtt aaggcaacga atacccacga
     7861 atgcacctga aacggcacaa aaaacagtga aattgccgtc accagaatgc caacaaacag
     7921 accacgcgcc acgccaccgc cgacatatcc ggcaataatg acgtgagtcg gaaccggcgc
     7981 taccagcagc tcttcaatat tacgctggaa cttggcacca aaaaatgatg acgcaacgtt
     8041 ggcgtaggca ttggtgatca ccgacatcat gatcagcccc ggtacgatga actgcatata
     8101 gctgaagcca tgcatatcgc caatacgcga accaatcagg ttaccgaaga taataaagta
     8161 aagggtcatg gtgatgactg gcggcaccag cgtctgcacc cagatacgca taaagcgatg
     8221 gatctctttc gcccagatgc tttttagcgc cacccagtaa agatgcatca tgcgcgatct
     8281 ccttgttttt cattaaccag tgaaacaaac agctcttcca gacggttagc tttgttacgc
     8341 atacttaata cctgaatgcc ctgctcactt aactgcgtaa atacgctgtt gatcccctgc
     8401 tcacgcagca cttcaacttc cagcgtcgcg gtatcgacca gtcgatactg atagccatcg
     8461 agcttcggta acggactttt cggtgcgaga tcgagaataa aggtttccga tttcagcttc
     8521 gccagcagcg ccttcatcga ggtattttcc accagctcac cgtgttgaat aatgccgata
     8581 ttgcggcaca gcatttctgc ttcttccagg tagtgtgtgg tgagaatgat ggtggtgcct
     8641 ttgtcgttta aatccttcaa aaagccccac attgagcggc gaagttcaat atccacgcct
     8701 gcggtcggtt cgtcgagaat cagtagttta ggttcatgca ttaacgcacg ggcaatcatt
     8761 aaacggcgct tcatcccgcc agataacata cgcgcacgtt cgttgcgttt tccccataga
     8821 tcgagttgtt taagatattt ttcgctgcgg atgtacgcct ctttgcgctc cacaccgtag
     8881 taccctgcct gattcaccac aatttgctgc acggtttcaa acgggttgaa gttaaattcc
     8941 tgcggcacca gtcccaactg acgtttagcg ttcacgacat ctttctcgag atcgtaacca
     9001 aatacgctga cccgcccgga ggttttattt accagagagc tgataatacc gatagtggtc
     9061 gatttcccgg ccccgttcgg cccgagaagc gcataaaaat cacccgcttc gacctgcaaa
     9121 tctattccac gaagcgcctg aacgccgcct ggataggttt ttttaagctg ttgaagttcc
     9181 agtgcaatgg tcataaattt ttacttacct tacgttctta cactttatct gtggtttaaa
     9241 tcgtcccgga gttgccctat attagccaaa cgtaattatt tggttacagg tcgttaacct
     9301 ccatgaaaga catagataca ctcatcagca acaatgcact atggtcaaaa atgctggtgg
     9361 aagaggatcc cgggtttttt gagaaactgg cacaagcgca aaaaccgcgc tttctatgga
     9421 ttggatgttc cgacagtcgc gttcctgcag aacgtttaac cggtcttgag ccgggcgaac
     9481 tctttgttca ccgtaatgtt gctaacctgg tcattcacac cgacctgaac tgcctttccg
     9541 tggttcagta tgcagtggat gtactagaag ttgaacacat tattatctgt ggccactacg
     9601 gttgcggcgg cgtacaagcc gcagttgaaa acccggaact ggggcttatc aacaactggc
     9661 tgctgcatat ccgcgatatc tggttcaaac atagctcatt gctcggcgaa atgccgcaag
     9721 agcgccgtct ggataccttg tgtgaactga acgtcatgga acaggtgtat aacctgggcc
     9781 actccaccat tatgcaatca gcgtggaaac gcgggcagaa agttaccatt cacggctggg
     9841 cctatggcat tcacgacggc ttgctgcgtg atctggatgt cacagcgact aaccgcgaaa
     9901 cccttgagca acgttaccgt cacgggattt ccaacctcaa gctgaaacac gccaaccaca
     9961 aataaaaatg ccatgccgga tgcaacacat ccggcaactt cacacttact cgtccagcag
    10021 aatcactttg ccgatatacg gcagatgacg gtaacgctgt gcgtaatcaa tgccgtaacc
    10081 caccacaaac tcatccggga tcgagaaacc gataaattct accgggacgt tcacttcacg
    10141 acgggacggt ttatccagca gcgtacaaat cgccagcgac ttcggttcgc gcaggcttaa
    10201 gatctcacgc actttcgaca gtgtattccc cgagtcgatg atatcttcaa caatcagcac
    10261 gtccttgcca cggatatctt catccagatc tttgaggatt ttcacatcac gggtggtgga
    10321 catgccgcta ccgtagctgg aggcggtcat aaagtcgact tcatgagata cctgaacttc
    10381 acggcacagg tccgccataa acataaatga gccacgcagc agaccaacca gcaccatatc
    10441 gctgccgctg tctttgtaac gctcagtaat ctgacgaccc agttcggcga tacgcgcttt
    10501 aatctccgct tcggggatca ttacttctac agtatgtttc atatctctaa ccatatgatt
    10561 taaaaacaaa tcacttcgca cgggcaggtt ttggcaccgc aacaaagcgc aagacacgca
    10621 gtataccagt aaaactattc attcgcggaa tctgttaata aagctcgaat tgtgatgacg
    10681 atcacacatg ttaaaaccta taattaattg ctactaaaat attaatgaat tgaaatggtg
    10741 tctctttatg gcaattaaca atacaggctc gcgacgatta ctcgtcacgc taacagccct
    10801 ttttgcagcg ctttgcgggc tgtatctact cattggcgga ggctggctgg tcgcgattgg
    10861 cggctcctgg tactacccta tcgctggtct tgtgatgctc ggcgtcgcct ggatgctgtg
    10921 gctcagtaaa cgtgccgcgc tttggctgta cgcagccctg ctgctcggca ccatgatttg
    10981 gggcgtctgg gaagttggtt tcgacttctg ggcgctgact ccgcgcagcg acattctggt
    11041 cttcttcggc atctggctga tcctgccgtt tgtctggcgt cgcctggtca ttcctgccag
    11101 cggcgcagtt gccgcactgg tggtggcact gctgattagc ggtggtatcc tgacctgggc
    11161 cggatttaac gatccgcagg aaatcaacgg caccttaagc gccgatgcca cacctgccga
    11221 agcaatctcc cccgttgccg atcaggactg gcctgcctat ggtcgtaatc aggaaggtca
    11281 acgcttttca cctctgaaac aaattaacgc cgataacgtt cacaagctga aagaagcctg
    11341 ggtgttccgt actggcgatg tgaagcagcc gaacgatccg ggtgaaatca ccaatgaagt
    11401 gacgccgatt aaagtgggcg acacccttta cctgtgtacc gctcaccagc gactgtttgc
    11461 gctcgatgcc gccagcggca aagagaaatg gcattacgat cctgagctga aaaccaacga
    11521 gtctttccag cacgtaacct gccgtggcgt ctcttatcat gaagccaaag cagaaacggc
    11581 ttcgccggaa gtgatggcgg attgcccgcg tcgtatcatt cttccagtga atgatggtcg
    11641 cctgattgcg attaacgctg aaaacggcaa actgtgcgaa accttcgcca ataaaggcgt
    11701 gcttaatctg caaagcaata tgccagacac caaaccgggt ctgtatgaac cgacttcgcc
    11761 accgattatc accgataaaa ccatcgtgat ggcaggttca gttaccgata acttctcaac
    11821 ccgcgaaacg tctggcgtga tccgtggttt tgatgtcaac accggggagc tgctgtgggc
    11881 ttttgatccg ggcgcgaaaa atccgaacgc aatcccgtct gacgaacaca cctttacctt
    11941 taactcgcca aactcgtggg caccagcggc ctatgacgcg aagctggatc tggtgtatct
    12001 gccgatgggc gtgaccacgc cggatatctg gggcggtaac cgcacaccgg aacaggaacg
    12061 ttatgccagc tcgattctgg cgctgaatgc cactaccggg aaactggcgt ggagctacca
    12121 gaccgttcac cacgacctgt gggatatgga tcttccggca cagccgacgc tggcggacat
    12181 caccgttaat ggtcagaaag tgccagttat ttacgctccg gcgaaaaccg gcaacatttt
    12241 tgtgctcgat cgtcgtaatg gcgaactggt ggttccggca ccggaaaaac cggttcccca
    12301 aggtgctgcc aaaggcgatt acgttacccc tactcaacca ttctcggaac tgagcttccg
    12361 tccgacgaaa gatttgagcg gtgcggatat gtggggagcc accatgtttg accaactggt
    12421 gtgccgcgtg atgttccacc agatgcgcta tgaaggcatt ttcaccccgc catctgaaca
    12481 gggtacgctg gtcttcccgg gtaacctggg gatgttcgaa tggggcggga tttccgttga
    12541 tccaaatcgt gaagtggcga ttgccaaccc aatggcactg ccgtttgttt cgaaactgat
    12601 cccgcgtggt cctggtaacc cgatggagca gccgaaagat gccaaaggca cgggtacgga
    12661 atccggcatt cagccacagt acggtgtacc gtatggtgtc acgctcaacc cgttcctctc
    12721 accgtttggt ctgccatgta aacagccagc atggggttat atctcggcgc tggatctgaa
    12781 aaccaatgaa gtggtgtgga agaaacgtat tggtacgccg caggacagta tgccgttccc
    12841 gatgcctgta ccggtgccgt tcaatatggg tatgccgatg ctgggcgggc caatctccac
    12901 ggcgggtaac gtgctgttta tcgccgctac ggcagataac tacctgcgcg cttacaacat
    12961 gagcaacggt gaaaaactgt ggcaaggtcg tttaccagcg ggtggtcagg cgacgccaat
    13021 gacctatgaa gtgaatggta agcagtatgt ggtgatctcc gcaggcggtc acggttcatt
    13081 tggtacgaag atgggcgact atattgtggc ttatgccctg ccggatgatg tgaagtaaga
    13141 cttgctcaga ttgctgacaa cgtgcgcgtt gttcatgccg gatgcggcgt taacgcctta
    13201 tccagcctac aaaattgtgc aaaatcaatg gattgcatag gtagcgtagg cctgataagc
    13261 gtagcgcatc aggcagattt gcgtttgtca tcagtttaat gcccggagag atccgggcat
    13321 atttccgaat acggtctttt tataccgtaa accctaacat catccccgta tcttcatgct
    13381 ccagcagatg gcagtgcgcc atataagcat gttctttcgg tgcatcgtga ttaaacttca
    13441 ccagcacttc gctgacatta ccttctacct taacggtatc tttccagccc gcgcgatgag
    13501 ccgctggcgg tttgccattt tctgacaaga tacggaactg cgtgccgtga atatggaacg
    13561 gatgcagcat catgtcaccc acgccagaga taacccaacg ttcgtactgc cctttcgccg
    13621 ccgcaaacat cggcttattc atatcaaacg cctgaccgtt gattttattg gcatggtgga
    13681 aatcgaactt cccgccgtgg ttcatatgat tcatattgcc gtgccccata tggcccatca
    13741 tctggctgtg atccatcccg gccatcgcct gatcgccata tttctccatc agcatctgca
    13801 tacccatcat atcgagcatc gggtccatag aaagttgcag cttgcgtacc gtcagccctt
    13861 ccagcgaagg taacgcaggc aggctactta atgtgtctgg caaagcaccg gaggcactaa
    13921 tagcaatcgg ctgaatccgc attaccggat gcggcttgtc aaacggcgca atcgccatcc
    13981 ccatctggct gaccggcagc gtcaccaggt caaagggttt gttgtcgtta acctccacca
    14041 gcacttcaaa acgctcgccc atcagcaccg gcagctcgtt caccttcacc ggttcaggta
    14101 gcagaccacc gtcgctggca atcacataaa gcgggcgatt gtcgctggtg gcgaaattga
    14161 gcgagcgggc attacagcca ttgagcaaac gcaggcgcag ccaaccacgc ggggcagcgt
    14221 gttgcgggta gattgcaccg ttggtcagca acgtatcgcc aaaccagccc acggcggcgg
    14281 tcatcacatc cagttgataa tcaatctgcc cgtcggcgct aaatttctta tcctgaacga
    14341 tcaccggaac atcatcgata ccccactgtt ttggcagcat taatttcagg atctcgtcat
    14401 cttcaattac caccaaccca gccaacccca tcgccacctg tcgcccggtc ttgccatgtt
    14461 gatgtggatg gaaccagcag gtagcggcag gttgatcaac gttcaacgtc accgagcgct
    14521 tgccacctgg cggaataatt ccctgcgggc cgccgtccac ttcacccggt acttccagcc
    14581 cgtgccagtg caacgtcgtc tcttccgtca gttggttata gatatcaacc gttaccgctt
    14641 tgccgtgctg taatttcacc gccggcccca gcagattgcc gttatagccc caggtagttg
    14701 cagttttccc gccaaaggtg gactggcctg cgccaatagt taactgaatg cgattacggg
    14761 catcggtcgt gagcaaatca gggatcggta acgttgggcg ttccgccgca aagactgcgc
    14821 ggctccacag cggcaaggct gaagccacac ccagcgcgac ggaatatttt aagaaatcac
    14881 ggcgttgcat agttatttcc ttattcttaa gcaggcgaaa caaaatcaaa cgttgagcat
    14941 agtccttccc cttacgggaa ggtcaagccg cagacacaat ttaatcgaca ataatggtcg
    15001 tcgcctcgct cgcagtgtgc taacgtttat cttctttaag cccctggtag aagcaatgaa
    15061 gacgtttttc agaacagtgt tattcggcag cctgatggcc gtctgcgcaa acagttacgc
    15121 gctcagcgag tctgaagccg aagatatggc cgatttaacg gcagtttttg tctttctgaa
    15181 gaacgattgt ggttaccaga acttacctaa cgggcaaatt cgtcgcgcac tggtcttttt
    15241 cgcccagcaa aaccagtggg atctcagtaa ttacgacacc ttcgacatga aagccctcgg
    15301 tgaagacagc taccgcgatc tcagcggcat tggcattccc gtcgctaaaa agtgcaaagc
    15361 cctggctcgc gattccttaa gcctgcttgc ctacgtcaaa taatccctga tacctttttg
    15421 tagaaatatt gaccgtgcat ccgcggtcaa tgttagctat tatgttgcgc ccttttttta
    15481 cgggtgttaa caaaggaggt atcaacccat ggccgaaaaa aaacagtggc atgaaacgct
    15541 acacgaccag tttgggcagt actttgcggt agataacgtt ctgtatcatg aaaagaccga
    15601 tcaccaggat ctgatcattt ttgagaacgc tgcatttggt cgcgtaatgg cgctggatgg
    15661 cgtagtacaa accaccgagc gcgacgagtt tatctatcat gagatgatga ctcatgttcc
    15721 gctactgacc cacggtcacg cgaaacatgt gctgattatc ggcggcggcg acggtgccat
    15781 gctgcgtgaa gtaacccgac ataaaaacgt tgagtcaatc acgatggtgg aaatcgatgc
    15841 gggtgtcgta tcgttctgcc gtcagtatct acccaaccat aacgccggta gctacgacga
    15901 tccgcgcttt aagctggtga tcgatgatgg cgtcaatttc gttaatcaaa ccagccagac
    15961 ctttgatgtc attatctccg actgcaccga tcctatcggt cccggcgaaa gtctgttcac
    16021 ctcagcattt tatgaaggct gcaaacgttg cctgaatcct ggcggtatct tcgtcgcaca
    16081 aaacggcgtc tgctttttac agcaggaaga agccatcgac agccatcgca aactcagcca
    16141 ttacttcagc gacgttggct tttatcaggc ggcgatcccg acctattacg gcggtatcat
    16201 gacttttgca tgggcgacag ataacgacgc cttacgccat ctctcaaccg aaattattca
    16261 ggcgcgtttt ctcgcttctg gcctgaaatg ccgttattac aatccggcaa tccatacggc
    16321 agcttttgcc ttacctcagt atctgcaaga cgcactggct tcacagccgt cctaaggaga
    16381 agataagaaa ttgaaaaaac tgaaactgca tggctttaat aatctgacca aaagtctgag
    16441 tttttgtatt tacgatatct gctacgccaa aactgccgaa gagcgcgacg gttatattgc
    16501 ttatatcgat gaactctata atgccaaccg tctgaccgaa atcctgtcag aaacctgttc
    16561 cattatcggg gccaatattc ttaacatcgc ccgtcaggat tacgaaccac agggtgccag
    16621 cgtcactatt ctggtgagtg aagaaccggt cgacccgaaa cttatcgaca aaacagaaca
    16681 ccccggccca ctgccagaaa cggtggttgc ccatctcgat aaaagtcata tttgcgtaca
    16741 tacctacccg gaaagtcatc ctgaaggcgg tttatgtacc ttccgcgccg atattgaagt
    16801 ctctacctgc ggcgtgattt ctccgctgaa ggcgctgaat tacctgatcc accagcttga
    16861 gtccgatatc gtaaccattg attatcgcgt gcgcggtttt acccgcgaca ttaacggtat
    16921 gaagcacttt atcgaccatg agattaattc gattcagaac tttatgtctg acgatatgaa
    16981 ggcgctgtat gacatggtgg atgtgaacgt ctatcaggaa aatatcttcc ataccaagat
    17041 gttgcttaaa gagttcgacc ttaagcacta catgttccac accaaaccgg aagatttaac
    17101 cgacagcgag cgccaggaaa ttaccgctgc gctgtggaaa gaaatgcgcg agatttatta
    17161 cgggcgcaat atgccagctg tttaacggct ctggcggagc tcccaggctc cgccagattt
    17221 atttacttct gctgcacgaa attgcggtaa gccgccacga cctgcagaaa atcctcaacg
    17281 ccgcatagcg acaggctttc ttcgtcgtag tagttcatcc cttcttccat ttcatcgcca
    17341 gcgaattcca gttgattggc gcgaaccatc acctcttcac cgtccatcca cagggtgtat
    17401 tcatgccctg cccgttgcca ggaccgttcg ctacctttca gtgcgtgcgc tgcttgttcc
    17461 acttcatcaa gcaaagccag gttttctttc acctcttcat taaaccagtg cccgaccact
    17521 tcatgcccca tggacatacg cacctttacc actccggtaa tatcgcgcag aaattcgtaa
    17581 tccatagtgt gttcctctgc tcccggcaat gcgccgaaat cgcattgcgc cattgatgta
    17641 attatcgcaa ccttaccgga gaaaaacagc ggaacggcga tggtttagaa ataaaaaatg
    17701 cccgggaatc tcattccccg agcattgtgt cgtttatgcg cagcgcgtgc gctgactttt
    17761 taaaccgcag tctggaaaat caccccatcg gctttttcgg tatactgaga aagctggttg
    17821 aagttcagat aacgataagt atcaacggca gttttatcca cttgcgccac gtaggtctgg
    17881 tactcttccg gcgtcggcag tttgccaatc agcgccgcaa cagccgccag ttccgcagaa
    17941 gccaggaaga cattcgcgcc agtacccaga cggttcggga agttacgggt agaggtggaa
    18001 accaccgtcg caccgtctgc tacacgcgcc tggttaccca tacacaggga acagccaggg
    18061 atctcgatac gcgcaccact cttaccgaag acgctgtagt agccttcttc agtcaactgc
    18121 gcggcgtcca tacgggttgg cggtgccacc cacaggcggg tcggcagttg gcctttgtgc
    18181 gcatccagca gtttacccgc agcacggaag tgaccgatgt tggtcatgca ggaaccgata
    18241 aatacttcgt cgatcttctc accctgtacc gcagacagcg gacgcgcgtc atccgggtcg
    18301 ttcggtgcac agaggattgg ctctttaata tccgccagat cgatgtcgat cactgccgcg
    18361 tattcagcat ctgcatcggc ttccagcagc tcaggattcg ccagccattt ttccatgccc
    18421 tgaatacgac gttccagggt gcgacgatcg ccgtaacctt ccgcgatcat ccacttcagc
    18481 aggacgatgt tagagttcag gtattcgatg atcggttctt tgttcagctt gatggtacaa
    18541 ccagcggcag aacgctcagc ggacgcatcg gtaagctcaa aggcctgctc aactttcaga
    18601 tccggcagac cttcaatttc cagaatgcgg ccagagaaga tgtttttctt gcctttcttc
    18661 tcaacggtca gcagaccttg tttgatggca tacagcggaa tggcgtgtac cagatcgcgc
    18721 agggtgatgc ccggctgcat tttgcctttg aagcgcacca gaacggattc cggcatatcc
    18781 agcggcatta cgcctgttgc ggctgcaaat gccaccagac cagaacccgc cgggaaagag
    18841 ataccgatcg ggaaacgggt atgggagtca cctccggtac cgacggtatc cggcagcagc
    18901 atacggttca gccaggagtg gattacgccg tcacccggac gcagcgacac accgccacgg
    18961 ttcataatga agtccggcag cgtgtggtgc gtgttcacgt caactggctt cggatacgcc
    19021 gcggtgtggc agaaagactg catcaccagg tcagccgaga agcccaggca cgccaggtct
    19081 ttcagttcat cacgggtcat cgggccggtg gtgtcctgag agcctacaga agtcattttc
    19141 ggttcgcagt acgcgcccgg acgaatgcct ttcacgccac aggcacggcc taccattttc
    19201 tgcgccagcg agaagccgcg atcgctctca gcgacatctt tcgcctgacg gaacacatca
    19261 ctgtgcggca gaccaagtgc ttcacgcgct ttggtggtca ggccacgccc gataatcagc
    19321 ggaatacggc caccagcacg cacttcatca atcagcacgt cggttttcag ttcgaaggtc
    19381 gccagcagtt cgccggtttc gtggttacgc acttcacctt tgtacgggta aacgtcaatc
    19441 acgtcgccca tgttcaggtt agagacgtcg acttcgattg gcagtgcacc cgcgtcttcc
    19501 atcgtgttaa agaagatggg tgcaatttta ccgccgaggc acaaaccacc gccgcgtttg
    19561 ttcggcacat gtggaatatc atcgcccata aaccacagaa cggagttagt ggcggattta
    19621 cgcgaagaac ccgtacccac aacgtcaccg acgtacgcca gcgggaaacc tttctgttgc
    19681 agagcttcga tttgcttgat cggaccaaca acaccaggct ggtctggctc aataccttca
    19741 cgggcgtttt tcagcatcgc cagcgcgtgc agtgggatat ccgggcgtga ccacgcatcc
    19801 ggtgccggag aaaggtcatc ggtgttagtt tcgccagtga ctttgaagac agtaacggtc
    19861 agtttttcag ccagcgccgg gcgattcagg aaccattcgg catccgccca ggactgcata
    19921 acctgcttcg catattcgtt gcctgctttc gctttctctt ctacgtcata gaagttatcg
    19981 aacatcagca gcgtgtgaga aagtgctttg gcagcaatag gtgccagttt ggcatcatcc
    20041 agcgcgtcga tcagcggatg aatgttgtaa ccaccctgca tggtgcccag cagttcgatg
    20101 gctttttccg gagtcagcag aggggatttg gcttcgcctt tcgcgatagc agccaggaag
    20161 cctgctttga cataggcggc ttcatcgacg cctgggggaa cacggttggt taacagatct
    20221 aacaggaatt cttcttcgcc cgcgggcggg tttttcagca gctctacaag tgcggccatt
    20281 tggtttgcat ccaggggttt gggcgcaatc ccctcagcgg cacgctcagc tacgtgctta
    20341 cggtattctt ctagcacgac ggttctcctc gctctcattg tcatagtgcg gcaggcgatt
    20401 ctcttcacgc tcctgtgaga cagcagtttg tagggtaaat gcccagtacc gcaacgggca
    20461 gaatagcagg attttgacga tgtgttaatc tgtttacaaa aaagcaacat aaaaaagtgg
    20521 ctgaatcgtt aaggatggtc tagagattgt ttccctccgt aaaaacttcg ttaattcgca
    20581 tggtgataat cacctttctc aacgaatccc aagattagtc aaaatttaaa ctaccgcctc
    20641 tttatactcg gattcacagc acctgcgggt ggcagttcgc cgaccattgc gatttccttg
    20701 agatccgaat tatgaaaatg actttgccgt ttaaacccca tgtgctggcg ctaatttgca
    20761 gtgccgggct ttgtgccgcc tctaccgggc tatatataaa aagccgcaca gtggaagcgc
    20821 ctgtggaacc gcaatcgaca caacagactg cgcctgacat caccgcagtt acgcttcctg
    20881 caacggtttc cgcaccgccc gtaacgcccg ccgtcgtcaa atccgcattc agcactgcac
    20941 aaatcgatca atgggttgcg cctgtcgcgc tgtaccccga ttctctgctt tcacaagtgt
    21001 taatggcatc aacctatccg gcaaacgttg ctcaagcagt gcaatggtcg cacgataatc
    21061 cacttaaaca aggcgatgct gctattcagg cggtatctga ccagccgtgg gacgccagcg
    21121 ttaaatcact ggtggccttt ccacaattga tggcattgat gggcgaaaac ccgcaatggg
    21181 tgcaaaacct gggcgatgct tttctggcgc agccgcagga cgtgatggac tcggt
//
LOCUS       DAMZET010000069        21181 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.069, whole genome shotgun sequence.
ACCESSION   DAMZET010000069 DAMZET010000000
VERSION     DAMZET010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.069"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..294)
                     /locus_tag="POB34_002593"
     CDS             complement(<1..294)
                     /locus_tag="POB34_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919516.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTS"
     gene            complement(736..948)
                     /gene="cspH"
                     /locus_tag="POB34_002594"
     CDS             complement(736..948)
                     /gene="cspH"
                     /locus_tag="POB34_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HDI5919517.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            1234..1446
                     /gene="cspG"
                     /locus_tag="POB34_002595"
     CDS             1234..1446
                     /gene="cspG"
                     /locus_tag="POB34_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HDI5919518.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            1457..1645
                     /locus_tag="POB34_002596"
     CDS             1457..1645
                     /locus_tag="POB34_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDI5919519.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            1620..1850
                     /gene="ymcE"
                     /locus_tag="POB34_002597"
     CDS             1620..1850
                     /gene="ymcE"
                     /locus_tag="POB34_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HDI5919520.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            1840..2013
                     /locus_tag="POB34_002598"
     CDS             1840..2013
                     /locus_tag="POB34_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HDI5919521.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(2062..3135)
                     /locus_tag="POB34_002599"
     CDS             complement(2062..3135)
                     /locus_tag="POB34_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDI5919522.1"
                     /translation="MAENKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHVAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(3207..5951)
                     /gene="torS"
                     /locus_tag="POB34_002600"
     CDS             complement(3207..5951)
                     /gene="torS"
                     /locus_tag="POB34_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HDI5919523.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTEGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITIEMLKTSGAQIVAVGNAAQALETLQNSEPFAAALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            6034..7062
                     /gene="torT"
                     /locus_tag="POB34_002601"
     CDS             6034..7062
                     /gene="torT"
                     /locus_tag="POB34_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HDI5919524.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(7035..7727)
                     /gene="torR"
                     /locus_tag="POB34_002602"
     CDS             complement(7035..7727)
                     /gene="torR"
                     /locus_tag="POB34_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HDI5919525.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            7857..9029
                     /gene="torC"
                     /locus_tag="POB34_002603"
     CDS             7857..9029
                     /gene="torC"
                     /locus_tag="POB34_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HDI5919526.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDMPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            9029..11575
                     /gene="torA"
                     /locus_tag="POB34_002604"
                     /pseudo
     CDS             9029..11575
                     /gene="torA"
                     /locus_tag="POB34_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            11572..12171
                     /gene="torD"
                     /locus_tag="POB34_002605"
     CDS             11572..12171
                     /gene="torD"
                     /locus_tag="POB34_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HDI5919527.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVVRCRQYDSFGFYAALSQLLLVLVESDHQNR"
     gene            complement(12264..12569)
                     /gene="cbpM"
                     /locus_tag="POB34_002606"
     CDS             complement(12264..12569)
                     /gene="cbpM"
                     /locus_tag="POB34_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HDI5919528.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12569..13489)
                     /gene="cbpA"
                     /locus_tag="POB34_002607"
     CDS             complement(12569..13489)
                     /gene="cbpA"
                     /locus_tag="POB34_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HDI5919529.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHSDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGHDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            13749..15008
                     /locus_tag="POB34_002608"
     CDS             13749..15008
                     /locus_tag="POB34_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HDI5919530.1"
                     /translation="MSSNIHGISCTANNYLKQAWNNIKNEHEKNQKYSITLFENTLVC
                     FMRLYKEIRRQKAEDYIPCLECDSLEKEFEEMQNDNDLSLFLRTLRTNDTETYSGVSE
                     GITYTIQYVRDIDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEWINTPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFNDLREDVKTLFGVNLQS
                     DNKIQGQGGFLSWSFLSFKKEFIDSCLNDKKCILHLPEFIFNDNKARLVLDTDPEQKV
                     NEVKNFLTALSDSICSLFIVNSNVASISLGNESFSTDDDLEYGYLINTGNHYDVYLPP
                     ELFAQAYELNNKERNAQIDFLTRYAIY"
     gene            15300..16541
                     /gene="agp"
                     /locus_tag="POB34_002609"
     CDS             15300..16541
                     /gene="agp"
                     /locus_tag="POB34_002609"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HDI5919531.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAQQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(16579..16806)
                     /locus_tag="POB34_002610"
     CDS             complement(16579..16806)
                     /locus_tag="POB34_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HDI5919532.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(16827..17423)
                     /gene="wrbA"
                     /locus_tag="POB34_002611"
     CDS             complement(16827..17423)
                     /gene="wrbA"
                     /locus_tag="POB34_002611"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDI5919533.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            17796..17969
                     /locus_tag="POB34_002612"
     CDS             17796..17969
                     /locus_tag="POB34_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDI5919534.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(18052..19380)
                     /gene="rutG"
                     /locus_tag="POB34_002613"
     CDS             complement(18052..19380)
                     /gene="rutG"
                     /locus_tag="POB34_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HDI5919535.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAIMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGVGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNLLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(19401..19895)
                     /gene="rutF"
                     /locus_tag="POB34_002614"
     CDS             complement(19401..19895)
                     /gene="rutF"
                     /locus_tag="POB34_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HDI5919536.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(19906..20496)
                     /locus_tag="POB34_002615"
     CDS             complement(19906..20496)
                     /locus_tag="POB34_002615"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HDI5919537.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSNLLINIGYGDNSKLYARLPRLSFEEACGLL"
     gene            complement(20506..>21181)
                     /gene="rutD"
                     /locus_tag="POB34_002616"
     CDS             complement(20506..>21181)
                     /gene="rutD"
                     /locus_tag="POB34_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HDI5919538.1"
                     /translation="VCYDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVG
                     HALGALVGMQLALDYPASVTVLVCVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQ
                     PLFLYPADWMAARAPRLEAEDALALAHFQGKNNLLRRLNALKRADFSHHAVRIRCPVQ
                     IICASDDLLVPSACSSELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLH
                     HREAAL"
BASE COUNT         5331 a         5318 c         5426 g         5106 t
ORIGIN      
        1 ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg ctgcaacaac
       61 tgccaacgtc gccacaccaa cagccgttgc cacaccgaca accgtcgccg tgccgacagc
      121 gccagcagcc acgccaccag tggtagccgc agtcgtggtt gcaccggtag tggtcgttgt
      181 ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct gcttcagggg cagcaaatgc
      241 agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt tcataacttt
      301 tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct actttgagta
      361 taacatcctt gtgaaatctc cctttcctca taccgcttgc actatatact gcgggtagga
      421 aaggcgcgca gagggaaata taagattgtt aactatcccc ctctggaaat acgacttgtg
      481 agcataagat aaatcttagc aaaatacaaa ttaaagataa ggaaattgtt acgaaagcta
      541 ttaattattg ttggtaatat agtttcaagt ggtactataa ctggctgtta aatggcattt
      601 ataactatta ggtgcagaga tattcgctta aatggattag tttctttgtg gaaaagtaac
      661 tgatgttata atgtatggtg ttgactattg cactattcca ataaaaccag tcagctttaa
      721 acaagcagcg tcatattaag agagataaac atttgccgct gttggtcctc gcaggccatt
      781 tacgcggcaa aattccacgc gtaatcctgg tataagcact tctgcgtcgc ggggagtgaa
      841 tgcggaaata tggacctgga cttctttacg accgtcggag gggataatga atcctttgcc
      901 gcttttgcga tcaaaggttt tgacaattcc tgtcatttta cgggacaaac aaattcctta
      961 ctgaaaatac tgtgccgcac tatacggggt taataaaata aagccagcga tatttaagac
     1021 cgccggacgg ctaaaataaa atttgcttaa tctcaattat catgcgttaa tagctgcgtc
     1081 ggtttgaaag acagacagca tacaaagtag tttactaaag cagttctcat tatcaggcat
     1141 tatccctttc ttttgagtct ctctcctgaa cactaagtag tttctgtatt aaagccctgt
     1201 ttgccgaaag gcccaaaatg aaggaagtaa aatatgtcta ataaaatgac tggtttagta
     1261 aaatggttta acgcagataa aggttttggc tttatcactc ctgatgatgg cagcaaagac
     1321 gttttcgtcc atttcaccgc cattcagagc aatgaattcc gcacgctgaa cgaaaatcag
     1381 aaagttgaat tttctattga gcaggggcaa cgtggccccg cggcagcgaa cgttgttacg
     1441 ctctaaggtt gccgttatta ctcaacatct ccatttccgc tgtccatgtt gtcatggttc
     1501 acagtaccgt acatcggcat tcgatgtgac ggagcgaaac cctttgggcg ctaagtgtat
     1561 tttttgtaaa tcgacgatga tcacctttga taacgtcgcg ctgcaaatac gcacggacca
     1621 tgcgccgctg gatttcacaa aataatatca ggctccctcg tggagccttt tttatatctg
     1681 ccttattttt cttcaacgct gtatgtatag taagcgataa cctgttgatt attgaatctt
     1741 tcggggagat ggcttataac atttcttacc tgaccagggt accgggaacc aacaccttac
     1801 tggcgtgttg ctgtctttca agaccagacg aggttaacag tgaatattga agagttaaaa
     1861 aaacaagccg aaacggaaat cgctgacttt atcgcgcaaa aaatcgccga gctgaacaag
     1921 aatacaggga aagaagtctc tgaaattcgc ttcaccgcac gagaaaaaat gaccgggctt
     1981 gaaagttatg atgtcaaaat caaaataatg tgattttgtg aacatcaccc cgtgcgaggt
     2041 gatgttccgc ttgttgctaa tttagtgacc aatcattggc gcttgtggaa ttaagcgtcg
     2101 gtacaattcc tccggcaccg ggctttgcca tactcccgca tacattgcgt aaccaatcac
     2161 cgcaaacata atccccagaa ccagtagcgt cattaaccag ccagacaacg caaaagcttt
     2221 tttatttgcc gcaggttttt gcagtgaaaa ggtcaatgtt gaggctaccg gacatgactc
     2281 tacgcaagtc atacagccgg tacattccac tgttcgtacc tgaattaatt tatctaccgg
     2341 gatccgtgat gggcaatttt ttgcgcattt gccacagtcg atacaacttt cggcattgcg
     2401 acgaatctta aacggcgaca atagcgaaac cacgcccatc agcgcgccat atgggcaaag
     2461 ataacgacac caggcatggc gaataaacag gctggcaatc agcaaaacgg tcacgctgat
     2521 taatgtcgcg gtccccatat gacgaaagaa atcgagcatt tttacgtcca tcaccacgct
     2581 gtagggcgac aacataaaat agtgaatcgc ctgagcgggc atcaataacg cgatatagag
     2641 aaaaaaactc aacagcaaat acttcacgcc gcgcagagga atatccagcc agcggggaag
     2701 gacacattgc cgaccaaaca gtttgttacc gagatcgccg attaattcag aaagcgtacc
     2761 aaccgggcat aaccatgagc aaaaggcctt tttgagtaat agactgatga cgataaaagc
     2821 gaccaataac agcatcgcgg cgacgtggac ggacggtaac tgacctgttg taaggctata
     2881 tttcagattc atcagaccgg caatcggtag ccagccttcg atacctcccg gtctggcgac
     2941 aaatgtcgtg ctacttgccg tttcgtaata gcgcacccaa taccagaacg tgatggcaat
     3001 ataaatattc attgccaaca gtaataattg cgtcgcttta cgccaggtcg tggcattacg
     3061 ccagtcattc cacggtaatt tgccgcccgt cgtgcctggc cgccgctgcc agcgggttct
     3121 tttattctct gccatgattt tgccagtccg ttaagttgta taccaaatgc cactattcta
     3181 gttgttctta actggctgat attgattcaa atcgcgttca ggtctttctt atgcaaccat
     3241 gcttccagag cggcaacact gcgtgtaatt tcttcgtgtg gaaggggggc agataatggc
     3301 tgctgctcca gttgtgcgca tagctggctg gcgatacgca ttcccagact cgagcaactg
     3361 ctttttagct gatgcgcggc acgctttatt ttctcgctat cctgactggc gcgggcaatg
     3421 tcgatttcat cgagaagcgg cagggcatgt tgtgtaaata ataccagcca ttcgtggatc
     3481 ttctccgtcc ccattaactg agcatcttca ttgagttgcg atacatccag cgattgatca
     3541 ttattgactt gcagttggag atagtgcgcc agtaactgac cgagcacttc acgcggcacc
     3601 ggtttaggga taatcccgcg gaataatgaa ctggtacgct ggcgcagcgt ttcgtcaatg
     3661 acatgggcgc taaagccaat caaaaccagc gacggatatt gctgtgccag ttgtcgggca
     3721 agcgtaatgc cgtcgatatc cggcagatca aaatccacca gtgcggcagc aaacggttcg
     3781 ctattttgca gtgtctctaa agcctgcgcg gcattgccaa cagcaacaat ctgcgcacca
     3841 ctggttttca gcatctcaat ggtaattcgc tgggttagcg ggttatcttc aattaacagc
     3901 aaacgtaaac cgtcaagacg caccgcctga ttgactgttt ttggcacggg tgccgtggca
     3961 acacgtaacg gcaagcgtaa acaaaaacag cttccaacct ccggcgtgct ggtggcgctc
     4021 agttcgccgc ccatcgcctg ggccagacgg ctactgatag tcagtcccag cccggtgccg
     4081 ccgcgtttgc cgcttacctg cacaaatggc tggaagattt ctgccagttt cgcgggatca
     4141 ataccgcagc cgctgtcttc cacttcgacc agccattgct cgccttcagt gcgactacgc
     4201 aggataatgt acccttcgtc agtaaaacgc agggcgttgc tcaacaggtt ggttataacc
     4261 tgacgaatac gtcgtggatc gcccattaac gcgcacggca tatcatcggc aattgccgtt
     4321 gccaggcgaa tcgggcgacc tttcacccgt ccgctcatta attgcagggt actttccagc
     4381 agcgggcgcg gttcaaaggg ctcatcgctg accgaaacat tcttgccacc tgcttcgata
     4441 gcggaataat cgagaatatc gttgaggatg gtcagcaacg attcaccaga gtcagtaatt
     4501 gcccgcaaat catcacgctg ggcgttaagt gcggggttat ctgccagtag ctgtgcggtg
     4561 ccgagaatac cgtacagcgg tgtgcggatc tcatggctca tcgccgccag aaacgccgat
     4621 tttgcctggc tggctttttc tgcttccgcc cgtgcctgtc ggtgttctat caccagttcc
     4681 tgcaattcag ctgtacgcgc tttgacctgc gccgccagct gttcgcggtg gcgattcagt
     4741 gcatgaacat tgctgcgaaa cgcatccatc agccgcccga tggtatccag ctcccgtacg
     4801 ccagcggttt ccgggaaagg ggagtcaata tcaccgtcca gcagccgttg cagcgcctgc
     4861 gtttgttcgg caagtggacg cgtgactgag cgataaacca cgcgccagag gatcagaatc
     4921 agtgcgcaaa gtgaaaccat ccccagcaat aacaggctgt attgcccgcg tgcactggct
     4981 ttttccagat gtgccagtcc atgctgattg cgcagctcaa tggtgtcgac cagctgactg
     5041 acttcgctac taaactgcgc gaactgggcg atattatttt gggcgagagt ttgcaggtga
     5101 ttgctgattt cactgtcctg ctggaacagc gccaacagat cgctatattg gctaacggta
     5161 gttaacgttg ttgcgacctg cgcacgaaca cccggatctt caatgcgtat ttgccgacgt
     5221 tgcagaattt tcaccgcatt attgagctgc ttttccagcg ttggcgcatt tttctggatc
     5281 tgctccagcc ccagattcat caccatttgc tgcacccgca gggcgctaag gcgcagttca
     5341 ttcatctggt taacatactc aagatcgata tcaatcagcc gatcgagtgc actttctgcg
     5401 gcctgacgct gatcttgttc gatcaaatcg taaatcccgg cctgggtcgc tccggcggag
     5461 gttgccgcat tattcgcctg accttgcgcc aggcgtgcga tctcatcggc ggcagcgact
     5521 atctgctgac tgagttgctg ttgttgctgg cgcagttgca aacgctgccc caccagttcc
     5581 ccttgctgac gtaacgaacg ggagatctcc tgctcctgtt gttcaatagc ggtggtatca
     5641 aaaccttgtt cccgtaacgc ttgcagcaac gcattaatct tcaggctttg tgcggtgagc
     5701 attcgcccct gcgcctgcca catcttttcg ttatcggcac tggtcaggtt ctgcgcggcg
     5761 aaaagttccc aggcgctggc ttcgctcaac tggcgcgcca tattcatggt aggaatcaat
     5821 gcctgagtat tgtctttttc cacctggctg ataaagcgca ggttgtacca tcccaccagg
     5881 gtactggtca gggttaacag cgccatcagg gcaaagccca tccagagtct tcgggtcagg
     5941 gttaaattca cggtcggtgc actttaggtg aaaaaggttg agtcgcaaag cggaatgcat
     6001 ctagcataaa gccttattat tgatgaggct atcatgcgcg tactgctatt tttacttctt
     6061 tcccttttca tgttgccggc attttcggct gataacctgt tgcgctggca tgatgcgcag
     6121 catttcacgg tgcaagcctc tacgccgctt aaagccaaac gcgcatggaa actgtgcgcg
     6181 ctttatccca gcctgaaaga ttcatattgg ttatcgttga actatggtat gcaggaggct
     6241 gctcgccgct acggtgtgga tttaaaagtg ctggaggcag gcggctacag ccagttggct
     6301 acccagcaag cacaaatcga ccagtgtaaa cagtggggcg cagaggccat tttgctcggt
     6361 agtagcacga cctcatttcc cgacctgcaa aagcaggtag caagtctgcc ggtgatcgaa
     6421 ctggtaaatg ctattgatgc tccccaggtg aaaagccgcg ttggtgtgcc ctggtttcag
     6481 atgggctatc aaccggggcg atatctggtg caatgggcgc acggtaaacc actgaatgtg
     6541 ctgttgatgc ccggacccga taacgccggg ggcagtaagg agatggtcga gggttttcgc
     6601 gcagccattg ccggaagccc ggtgcgtatt gttgatattg cgcttggtga taacgatatt
     6661 gaaatccagc gtaacctgtt gcaggagatg ctggaacgcc atccagaaat cgacgtcgtt
     6721 gccggaacgg ccattgcggc agaggcggca atgggggaag ggcgtaacct gaaaacgccg
     6781 cttaccgtgg tgtcgtttta tctttcacat caggtgtatc gcgggctgaa gcggggaaga
     6841 gtgattatgg ctgccagcga tcaaatggtc tggcaggggg aactggcagt tgagcaggcc
     6901 atcaggcaat tacaggggca atcggtgtct gataatgtca gcccaccgat tttagttctg
     6961 acgccgaaaa atgccgaccg agaacatatc cgccgctcgc tgtcaccggg gggatttcgt
     7021 ccggtctatt tttatcagca cacatcagcg gctaagaaat aaccttcacc atgttgcgtc
     7081 accagtaaat ccgcgctgag tttatgacgt aaacgacgaa ttaacacatc gacggtgcgc
     7141 aggtcagggt tttccacccg acgcgcagaa agcatacgta gcagacgttc acggctgaga
     7201 atttcgcccg gattcgtcac aaatgccacc aacatttcat actctgcgcg ggtcagttta
     7261 atcggctcgc catcccgctc cagcgtatgg cgcgacacat tcaggcaata accggcaaag
     7321 cgatagcagt tgtcctgagt gtgcggttga gcttgtcgcg cgaggtcgat tcgccagagc
     7381 agatttttca cccgtactac cagttcgcgc agttccagcg gtttggtgac gtaatcgtct
     7441 gcgcccattt ccagcccaac aatacggtca atccgatcgc tgcgtccggt aaccagaata
     7501 atccccaccg ttgagcgttc tcgcagggcg cgggttaaca tcaggccatt ttcatcgggt
     7561 aagttgatat ccagcagaat taaatctacc ggctgattct gcataatttc ccgcagcccg
     7621 gcaccgctcg ctgtaacgga aacggtatac ccctcctgag tgaagtaaga ttgtaatcgc
     7681 gcctgggtaa ccggctcatc ttcaacaata acaatgtgat gtggcatcag agggttttac
     7741 tcattctgtt catatctgtt catattctgc cgtaagccgt tcatcctgac cagtgccgct
     7801 gttcatattt gctcattaag atcgcttcac taaaccataa ttttacaggg gctattatgc
     7861 ggaaactctg gaacgcgcta cgccgaccca gtgctcgttg gtcggtactg gcgctggtcg
     7921 caattgggat tgtgattggc attgcgctga ttgtattgcc acacgttggg atcaaagtca
     7981 ccagcacaac cgaattttgt gtcagttgcc acagtatgca accggtgtat gaagaatata
     8041 aacagtcggt gcatttccag aacgcctccg gcgtgcgagc tgaatgccat gactgtcata
     8101 tcccgccgga tatgccaggc atggtgaagc gtaaactgga agcgagcaat gatatctacc
     8161 agacctttat tgctcactcc attgatacac ctgaaaaatt cgaagccaaa cgcgcggaac
     8221 ttgccgagcg tgaatgggcg cgaatgaaag aaaacaactc ggcaacctgc cgctcctgcc
     8281 ataactacga tgcgatggat catgcgaagc agcatcctga agcggcacgt cagatgaagg
     8341 tggcagcgaa agataatcaa tcctgcatcg actgtcataa aggtattgcc caccagttac
     8401 cggatatgag tagcggcttc cgtaagcagt tcgatgagct gcgcgccagt gctaatgaca
     8461 gtggtgacac gctgtactct attgatatta agccaattta tgcggcgaaa ggcgataaag
     8521 aagcctctgg ttctctgctg cctgcttcgg aagtgaaagt ccttaaacgt gacggcgact
     8581 ggctgcaaat tgaaattacc ggctggacgg aaagcgccgg acgtcagcgt gtactcaccc
     8641 aattcccagg taaacgcatc tttgttgcct cgattcgtgg tgatgtgcag cagcaggtaa
     8701 aaacgctgga gaaaaccacc gttgccgaca ccaataccga gtggagcaag ttgcaggcca
     8761 ctgcgtggat gaagaaaggc gacatggtga acgatatcaa accgatctgg gcttatgcgg
     8821 attcgttgta caacggcacc tgtaaccagt gccacggcgc accggaaatc gcccactttg
     8881 acgctaacgg ttggatcggc acgctcaacg gcatgattgg ctttaccagt ctcgataaac
     8941 gtgaagaacg caccttgttg aaatatctgc aaatgaatgc gtctgacacc gcaggtaagg
     9001 ctcacggcga taagaaggaa gaaaaataat gaacaataac gatctctttc aggcatcacg
     9061 tcggcgtttt ctggcacaac tcggcggctt aaccgtcgcc gggatgctgg ggccgtcatt
     9121 gttaacgccg cgccgtgcga ctgcggcgca agcggcgact gacgctgtca tctcgaaaga
     9181 gggcattctt accgggtcgc actggggggc tatccgcgcg acggtgaagg atggtcgctt
     9241 tgtggcggca aaaccgttcg aactggataa atatccgtcg aaaatgattg ccggattgcc
     9301 ggatcacgta cacaacgcgg cgcgtattcg ttatccgatg gtacgcgtgg actggctgcg
     9361 taagcgccat ctcagcgata cctcccagcg cggtgataac cgttttgtgc gcgtgagctg
     9421 ggatgaagcc ctcgacatgt tctatgaaga actggaacgc gtgcagaaaa ctcacgggcc
     9481 gagtgccttg ctgaccgcca gtggttggca atcgacgggg atgttccata acgcttcggg
     9541 gatgctggcg aaagctattg ccttgcatgg taatagcgtt ggtacgggcg gagattactc
     9601 taccggtgct gcgcaggtga tcctgccgcg cgtggttggc tcaatggaag tgtatgaaca
     9661 gcaaacctcc tggccgctgg tattgcagaa cagcaaaacc attgtgctgt ggggctctga
     9721 tttgctgaaa aaccagcaag cgaactggtg gtgcccggat cacgatgttt atgaatatta
     9781 cgcgcagcta aaagcgaaag tcgccgccgg tgaaattgag gtcatcagca tcgatccggt
     9841 tgtcacatcc acccatgagt atctggggcg cgagcatgtg aagcacattg cggttaaccc
     9901 gcaaactgat gtgccgctgc aactggcgct ggcgtatacg ctgtacagtg aaaacctgta
     9961 cgacaaaaac ttcctcgcta actactgtgt gggttttgag cagttcctgc cgtacctgct
    10021 gggtgagaaa gacggtcagc cgaaagatgc cgcatgggct gaaaaactga ccggcattga
    10081 tgcctaaacc attcgtgggc tggcgcggca gatggcggcg aacagaacgc aaattattgc
    10141 tggctggtgc gtgcagcgta tgcagcacgg tgaacagtgg gcgtggatga ttgtggttct
    10201 ggcggcgatg ctggggcaaa ttggcctgcc aggtggtggc tttggttttg gctggcacta
    10261 taacggcgca ggcacgccgg ggcgtaaagg cgttattctt agtggtttct ccggctctac
    10321 gtcgattccg cctgttcacg acaacagtga ttacaaaggc tacagcagca ccattccgat
    10381 tgcccgtttt atcgatgcga tcctcgaacc ggggaaagtg atcaactgga acggtaaatc
    10441 ggtaaaactg ccgccgctga aaatgtgtat ttttgccgga actaacccat tccatcgcca
    10501 tcagcagatc aaccgcatta ttgaaggctg gcgcaagctg gaaacggtta tcgccataga
    10561 taaccagtgg acctcaacct gccgctttgc cgatatcgtg ctgcctgcga ccacgcagtt
    10621 tgagcgtaac gatctcgacc agtacggcaa ccactccaac cgtggcatta tcgccatgaa
    10681 acaggtggtg ccgccgcagt tcgaggcgcg caacgacttt gatattttcc gcgagctgtg
    10741 ccgtcgcttt aatcgcgaag aagcctttac cgaagggctg gacgaaatgg gctggctgaa
    10801 acgcatctgg caggaaggtg tacaacaagg caaaggacgc ggcgttcatc tgccagcgtt
    10861 tgatgacttc tggaataaca aagagtacgt cgagtttgac catccgcaga tgtttgttcg
    10921 ccaccaggca ttccgcgaag atccggatct cgaaccgctg ggcacgccga gtggcctgat
    10981 tgagatctac tcgaaaacca tcgccgatat gaactacgac gattgtcagg ggcatccgat
    11041 gtggtttgag aaaatcgaac gctcccacgg tgggcccggc tcgcagaagt atccgttgca
    11101 tctgcaatct gtgcatccgg atttccgact tcactcgcag ttatgtgagt cggaaactct
    11161 gcgtcagcaa tatacggtag cgggtaaaga gccagtgttc attaacccgc aggatgccag
    11221 cgcgcgcggt attcgtaacg gtgatgtggt acgcgtcttt aacgctcgcg gtcaggtgtt
    11281 ggcaggggca gtagtttctg accgctatgc acccggcgtg gcacgaattc acgaaggggc
    11341 atggtacgat ccagataaag gcggcgagcc tggtgcactg tgcaaatacg gtaaccccaa
    11401 cgtgctgacc atcgacatcg gtacttcgca gctggcgcag gcgaccagtg cgcacactac
    11461 gctggtggaa attgagaagt acaatggagc agtggagcag gtgacggcgt ttaacggccc
    11521 tgtggagatg gtggcgcagt gcgaatatgt tcccgcgtcg caggtgaaat catgaccacg
    11581 ctgacagcac aacagattgc ctgtgtttac gcctggctgg cgcagttgtt ctcccgtgaa
    11641 ctggacgatg aacaactgac gcaaatcgcc agtgcgcaga tggctgaatg gttttcgttg
    11701 ctgaaaagcg aaccgccgct ggctgcggcg gtgaatgagc tggaaaactg tattgccacg
    11761 ctgacagtac gtgacgatgc ccgtctggaa ctggccgctg acttttgcgg cctgtttctg
    11821 atgaccgaca aacaagcggc gctgccgtat gcatcggcct acaaacagga cgagcaagag
    11881 attaaacgct tgttagttga ggcagggatg gaaaccagcg gcaatttcaa cgaaccggca
    11941 gatcatctgg cgatctatct cgaattgctc agccatctgc atttttcgct gggagagggg
    12001 accgttcctg cgcgaagaat cgacagtttg cggcaaaaaa cactgacggc gctgtggcaa
    12061 tggttaccag agtttgttgt gcgttgtcgt cagtatgaca gctttggttt ttacgcggca
    12121 ctaagccagt tattgctggt gttagtggag agcgaccacc aaaacagata acgtcgtttg
    12181 tgcgcctgaa aagacgcgtt tagcgtcgca tcaggcatta tggggcagtt gccggatgcg
    12241 tcatcacatc cggcaaacac acatcacgga tgagctacaa accgggaaag tcgctggcgc
    12301 agcaggcggt tttcctgctt caggtgcgca atatcatcca ttaacgtcag cgccaccgcg
    12361 atcccaggcc agtccagagc cagttcatga cgcaggcgta ctgcgcgttg caccacgatg
    12421 gcggcatggt cgtcaaatac ccaggttgtt tcctggatct cacgcggttc aaccaccccc
    12481 aaaccgacaa tttcattcaa ctcctcttca gagatgccgg tatgcaggca aaattcggta
    12541 atagtaaaag tcaccgtaac attagccatt atgctttccc ccaatcttta cgtggatcaa
    12601 aagacgactg ggcgtctgcc agttgctgcc acagcgcggc agtgttttca tccggtttcg
    12661 gcggcatcac gattttcagt accgcataca gatcgccggt ctgttttttg ctcaccagac
    12721 ctttgccttt aacgcgcaat cgttgcccgg cctggctgcc tggcgggata gtcagcaaaa
    12781 tgctttcttt cagtgttgga acggtgactt tagcacccag cgccgcttcc cacgggctaa
    12841 ccggcactac aatttccaga tcatggccga caatatcgaa cagcggatgt ggcgcaatat
    12901 gaatcaccag ccacaaatcg ccatttggac cgccgttttc gcccggcgtc ccctggcctt
    12961 tcagacggat gcgttgacca ttgccgacgc ccgccgggat cttgacattc agcgttttcg
    13021 gaatttcctg ttcgatcatg ccaaaggcgt tataaaccgg caggttatag ctgatggtac
    13081 gcttatgctc agtaagcgtt tcttcgagga ataccgccac ttcgatttca atatcgtggc
    13141 cgcgtgtggc ggggcgttga cggctctggc gggcatgctg accgaaaatt gacgagaaga
    13201 tatcgtcaaa atcttcggcg ttaaaactct gaccgtcgct atgatggaac tgacggttaa
    13261 attgcggatc gttgcgatgt tgccacatct gatcatactc agcgcgacgt tgttcatcac
    13321 ttaacacttc ccaggcttca gcgacctctt tgaagcgggc ttcggcatcc ggttctttgc
    13381 tgacatcagg atggtatttg cgggccagtc gacgataggc ggtcttgatt gtcttgagat
    13441 cgtccgtcgg tttcacgccc atgatggcgt aataatcctt taattccata gcgttatctc
    13501 gcgtaaatca acacaaattg aaggaacccc tgtaaggtaa ctcctataag tgtagggtaa
    13561 tcctcaaaat ttcatatgcc aacacagaat atgttattga aatcatcgcg gagaggaggt
    13621 cgcctcaagg tggggtgctg aacatatttt aaacaggtga aaaagggtga gcgatttttg
    13681 atagtggaac caggcacttt aagtttaact ggggcgtcat tatttattaa attttatagg
    13741 cgctatatat gagtagtaat atacatggaa ttagttgcac tgcaaataat tatttgaaac
    13801 aagcctggaa caatataaaa aatgagcacg aaaaaaatca aaaatattca atcacgcttt
    13861 ttgaaaacac actggtgtgt tttatgcggt tgtataaaga aatcagacgt caaaaagctg
    13921 aagactacat tccatgcctg gaatgcgatt cactagaaaa agaatttgag gaaatgcaga
    13981 atgataatga tttatcatta tttttgagaa cattgcgtac taatgataca gaaacttatt
    14041 caggggtttc agaaggtatt acatatacta tacaatatgt tcgagatatt gatattgtta
    14101 gagtgtcctt gccgggcaga ggttcagagt ctatcacaga ttttaaaggt tattattggt
    14161 atggctttat ggaatatatt gaaaacatta atgcgtgtga tgatgttttt tctgagtatt
    14221 gtttagatga tgaaaatatg agtatccagc cagaatggat aaatacgccg ggaatatctg
    14281 atttagatac tggtattgat ttgtctggta tatcttttat tcagagtgaa attaataaaa
    14341 cttatggatt aaaatacgct ccagtagatg gcgatggcta ttgtctgtta agggccatac
    14401 tggttttaaa agaacatgag tattcatggg cgctgggcag ccataagacg caaaagcaag
    14461 tatacgaaga gtttattaaa attgttgata aacaaacgat cgaggctctt gttgatacgg
    14521 cattcaatga tctcagggaa gatgtaaaga cattattcgg cgttaatcta caatctgaca
    14581 acaaaattca ggggcagggt ggttttttat catggagttt tttgtctttt aaaaaagaat
    14641 ttattgatag ttgcttgaat gacaaaaaat gcatactgca tttacccgag tttatattta
    14701 atgataataa ggcacgcctt gttttagaca ccgacccaga gcagaaggtt aatgaggtga
    14761 aaaatttcct cactgctctt tcagatagca tttgctcatt atttattgtt aatagtaatg
    14821 tggcatcaat ctccttaggg aatgaatcct tttcaacaga tgacgacctt gagtatgggt
    14881 atttaataaa cactggcaat cattatgacg tttacctccc tcctgaactt tttgctcagg
    14941 cttacgagtt aaacaataag gaaaggaatg cgcaaattga ttttttaact cgttatgcaa
    15001 tttactgaca aaggcatgtg ctaaaaacca ttgttattag cctcacttct cttattattg
    15061 gtactattgc ctctgtatta gtaacgccgc tgatattctg tttagccaca ggtgtaatca
    15121 tcagcggcgt acgcgaggca gggagctaat caggcatagt ttgcgtcaaa ccttgcctgt
    15181 ttttaaagat gtatatagaa aaacaggcat tcaacaagcc attttgcgaa cctgttcccg
    15241 gaaaaatgtc atatttctgt cacactcttt agtgattgat aacaaaagag gtgccaggaa
    15301 tgaacaaaac gctaatcgcc gcaactgtgg cagggatagt tttactcgct tcaaatgctc
    15361 aggcacaaac cgtaccggaa ggctatcagc tacagcaagt gctcatgatg agccgccata
    15421 acttacgtgc gccgctggcg aacaatggca gtgtgctgga gcagtctacg ccgaataaat
    15481 ggcccgaatg ggacgtcccc ggtgggcaac tcaccaccaa aggtggcgtg ctcgaagtgt
    15541 atatgggcca ttacatgcgt gaatggctgg cacagcaggg gatggtgaaa tcgggggaat
    15601 gcccgccgcc ggacaccgtt tatgcctatg ccaatagtct gcaacgtacc gttgcgaccg
    15661 cacagttctt tattaccggc gcattcccgg ggtgtgatat tcctgtgcat caccaggaaa
    15721 aaatgggcac tatggaccca acctttaacc cggtgatcac cgatgattcc gccgcattca
    15781 gtgaacaggc ggtggcggca atggagaaag agctcagcaa actccagctt accgacagct
    15841 accaactact ggaaaaaatc gttaactata aagattcccc tgcctgtaaa gagaaacaac
    15901 agtgttcgct ggtggatggc aaaaatacct ttagcgccaa gtatcaacaa gaaccaggtg
    15961 tttccgggcc gctgaaagtc ggcaactcgc tggtagatgc gtttactttg caatattacg
    16021 aaggttttcc gatggatcag gtggcctggg gagaaatcaa atctgaccag cagtggaagg
    16081 tgttgtcgaa gcttaaaaac ggctaccagg acagcctgtt tacctcaccg gaagtggcgc
    16141 gcaatgttgc gaaaccgctg gtcagttata tcgacaaagc tctggtcacc gatcgcacca
    16201 gcgcaccgaa aattacagtg ttggttgggc acgactccaa cattgcctct ctgttaacgg
    16261 cgctggattt caaaccgtat cagttgcatg accagaacga acgcacgccg attggcggca
    16321 aaatcgtttt ccagcgttgg catgacagca aagccaatcg cgatttgatg aaaattgaat
    16381 atgtgtatca gagtgcggaa cagttacgta atgccgatgc gttaaccctg caggcacctg
    16441 ctcagcgtgt gacgctggaa ttaagcggtt gcccgataga tgccaacggt ttctgcccaa
    16501 tggataagtt tgatagcgtg ttgaatgagg cggtgaaata acagaaaact cccccgcgag
    16561 aagtggggga gtcgctggtt aaacgttttt acgttcgatg gtctgttcgc cccaaaaaag
    16621 cgaatcttta tcggtcttag caaaggcttt gactaacact tcatcgctac cttcttccca
    16681 aatcttttcc gccatttttt cgtcgtaccc ggcgacttcg aaaatggcct cggctatttc
    16741 cggcgacgta ttgcgcagag atgcccattc accgacgtga tgagctttcg cttcttgagt
    16801 tggcatgcgt atcctcctgt tgaagattag ccgttaagtt taactgccag acctgcgaca
    16861 tattcccctt gataacgagc aatagacagt tcttcctggc ttggctggcg tgagccgtca
    16921 ccgcctgcga tggtggttgc gccgtacggc gtaccgccgc gaacctgtga aacgtcaaat
    16981 aattcctgcg ctgcgtagcc aatggggaca attaccatgc cgtgatgcgc aagggtcgtc
    17041 caggttgaag taatagtttg ttcctgaccg ccgccagtac cggtggaact aaagacgctc
    17101 gccagttttc cgtatagtgc gccggaagcc cacaggccgc ccgtctggtc gaggaaggta
    17161 cgcatttgac cggacatgtt gccaaagcgg gtaggtgtac caaaaataat ggcgtcgtaa
    17221 tcggccagtt cttgcggggt tgcaaccggt gcagtttgcg ttttaccgcc tgctttttca
    17281 aataattgcg gcggcatggt ttccggtaca cgcttaacga caacttctgc gccatcgact
    17341 ttgcttgcac cctcagcgac tgcgcgtgcc atcgtttcaa tatgtccgta catggaataa
    17401 taaagcacca gaactttagc catttctaac cactcctcgt gttatctcta ttccgtagcg
    17461 attcgctacc acttatttaa agataagacg tccttttcag agtgcaaatt tcacaaccac
    17521 ttatttgatt tataacaact ttcacaagca cgtaattttg tcgcaaaatg acacattttt
    17581 atctcatcgc gtttttttaa tcataagagc ggcttatgga taattatttg agatgatatc
    17641 tattctcgct aagaagctgt tgcaggatat taccaaacgc gggtctgccc gcgtcagttc
    17701 actaagctta gtcccacgta gcgaaaatat ggcagccgcc atacgccgcg ttaattctat
    17761 gcaatatgat gtctataccc agacggaggt cagtaatggc aaaccatcga ggcggttccg
    17821 gcaattttgc agaagaccgc gaaagagcat cagaagcagg taaaaaaggt ggacagcaca
    17881 gcgggggtaa tttcaagaat gacccgcagc gcgcatctga agcaggtaaa aaaggtggca
    17941 agagcagtca cggcaaaagc gacaactagc cgggctaatc aatgacgaat gcattttttt
    18001 cttaggctcg tcaaaaagtc atcaccgccg gtaatcccgg cggttttctg tttatggctc
    18061 ctgatgaaca acttctggcg gtggaacgtc aaccaacttt ctgcttaaca acgcattgag
    18121 taaaatcgcg ccaaaggttg ctgtaccaat ccctcccaac gtaaaaccgc ccagcgtgag
    18181 agcaaaatca cccgcgccca gcactaaggt tactgcgacc ataatcaaat taccgttctg
    18241 gcttaaatcg acacggtttt gtacccatat ccttgcgcct gcgacggcaa tcagcccgaa
    18301 cacaacaatt gatgcaccac caatcaccgc ggccggaatg gtatgaatca gcgcgccaaa
    18361 tttcggtgaa aagcccaaca gcatggcgat gacggcagca gcgacaaaca ccagcgtcga
    18421 gtagactttt gtcacggcca ttacaccgat attctcggcg taggtggtta cgccgctgcc
    18481 accgacagaa ccagaaagca tcgtcgccag cccatcaccg acgaatgccc gccccatata
    18541 cgggtccata ttgcgtccgg tcatcccggc gaccgccttg agatgaccta agttttccgc
    18601 caccagaatc accgccacgg gcgcaatcag catcattgcc tgactattaa aagcaggagt
    18661 ggaaaaatgt ggcaaaccga accaggcagc atggctgacg agagtaaaat cgacggcttt
    18721 tcccagccct aaaaggttgg tcatcacgcc atacagcaga caggcgacaa ttaaacctac
    18781 gagaatcaat aaccgctgga tcatgccacg ggtaaacacc gccaccagcc caatacacag
    18841 caccgtcatt accgccatcc agctatcaaa ggccgaagcc gatacacttt tcactgcgat
    18901 aggcgctaag ttcaggccaa tcgccatcac caccgcaccc gtcaccaccg gcggcattag
    18961 tcgttcaatc cagcgtgtac cgattttcat caccaccagg ccaatgacgg tataaaccag
    19021 cccacaggcg ataatcccgc ccagcgcaat gctgatattc gggttaatgc cctgaccgtt
    19081 aaagcccgtc gcggcaatca ccacgccgac aaaagccgcg ctggaaccga gataactggg
    19141 gacgcgcccg ccggtaataa agaaaaacag taacgtgccg acccccgaca ttaaaatgga
    19201 aaggttagga tccagcccca taagaatcgg cattaacacc gtcgcgccaa acatcgccac
    19261 cgcgtgttga acgcccatta ttgccgtctg agcaaacggc aatcgttcat ccggcgcgac
    19321 cacgccgctc tctgtagagg tcgattttaa ctgccagtga ggaaaaccga acattgccat
    19381 cagctgtctc cttaaggagg ttaacaagca gggcgcatca gcgcgtgata actgcgatcg
    19441 aaccacacca gcccgtaggg tgtggcgtga cgatgaatcg cttcgatggc gcaaaacaga
    19501 atgtcgtggg tgccgacgct caccacctgg ctgatacggc agtcaaacga aaccagagcc
    19561 tcttccagtt gcgggcatcc ggtcacaccc gtctgccagc gggcggcggc aaagcggtgt
    19621 tccatgggcg ttttgccgcc aaaaaggttg gaaagcggct cctgcccggc gctaagtgta
    19681 tttacacaca gcgttcgatt ttcattgaat accggccaga cggacgcccc acgattcagg
    19741 cacaccagta atgtgggcgg cgtgtcggtc acgctgcaga cggcgctggc ggtgaacccg
    19801 gcgcgcccgg ctggaccgtc cgtggtgatg atattgaccg ccgcgcccat acaggacatc
    19861 gcatcgcgaa aggtttgttg atcaactatg ttcatagttt gctccttaca acagcccgca
    19921 ggcttcttca aaggacagac gtggcaggcg cgcataaagt ttgctgttat cgccatagcc
    19981 gatgttaatc agcagattgc tcttcagcgt gctgcctgca aaaaaagcgt cgtcaacgta
    20041 ttgacggtca aaacccgaca tcgggccggt atccagtccc agcgcacggc aagcgacgat
    20101 cagataggcc gcctgcatgg aactgttgcg aaacgctgtt tcttcggcaa gttgtgggct
    20161 ggaggtaaac caactgcggg catcaccgtg gggaaacagt agtggtaacc gttcataaaa
    20221 ttcactgtcc caggcgacga tagcggtgac gggcgcggtc agggtttttt gcagattgcc
    20281 gctggaaagc gccgggcgca gacgttcttt tccctctgca gtgcggataa acacgatacg
    20341 tgccggagaa cagttagctg atgtcggccc ccatttcatc agggcataaa gctcccgtaa
    20401 cgtctcatcg ctgacgggtg tctcccgcca gccattgtga gtgcgggcat cggtgaacag
    20461 ggtgctaagc gcacctgggc taacggcttc gttcatagca attccttaca gggcggcttc
    20521 acggtgatgt aacaggctgg caagcccgtt gagtaacaga gcattaaacg tttcgggatc
    20581 ggtcacgttg caggcgtgtc cgccatagcg catcaccatt ttctggctat cgggcagggc
    20641 ggcatgaagt tcactggaac atgctgatgg caccagcaga tcatcactgg cgcagatgat
    20701 ttgcaccggg cagcggatgc gaaccgcatg atgactaaag tcagcgcgtt tgagggcgtt
    20761 aagtcgacgc agtaaattat ttttgccctg aaaatgcgcc agtgccagcg cgtcttctgc
    20821 ctcaaggcga ggtgcgcggg ccgccatcca gtcggcggga tagaggaaca acggctgcgc
    20881 ttccacccat gcctgcgcgc cgccgctata cagtaatcgt tcgcgaacct gaaaacagcg
    20941 gcgcgtatgg gcgtttattc gtaaccagcc gttaacgcag accagcacag ttaccgacgc
    21001 gggataatcc agcgccagct gcatccccac cagcgcaccg agcgcatggc cgaccactgc
    21061 gtaatgctca atccctgcgg ctaccagcgc ctgatgcagt tccgctgcca tctgggcgat
    21121 actgtaatct tctgccagcg tgtcgggatt attgccggtg ccgcgctggt cgtaacagac
    21181 t
//
LOCUS       DAMZET010000070        21080 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.070, whole genome shotgun sequence.
ACCESSION   DAMZET010000070 DAMZET010000000
VERSION     DAMZET010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.070"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..474)
                     /locus_tag="POB34_002617"
     CDS             complement(<1..474)
                     /locus_tag="POB34_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDI5919539.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHH"
     gene            complement(496..2508)
                     /gene="hyfR"
                     /locus_tag="POB34_002618"
     CDS             complement(496..2508)
                     /gene="hyfR"
                     /locus_tag="POB34_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HDI5919540.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATVEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(2538..2951)
                     /gene="hyfJ"
                     /locus_tag="POB34_002619"
     CDS             complement(2538..2951)
                     /gene="hyfJ"
                     /locus_tag="POB34_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HDI5919541.1"
                     /translation="MAEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFALLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(2944..3702)
                     /gene="hyfI"
                     /locus_tag="POB34_002620"
     CDS             complement(2944..3702)
                     /gene="hyfI"
                     /locus_tag="POB34_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HDI5919542.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPLQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLREADDPRLNSIVQQLFRVLRGLHG"
     gene            complement(3699..4244)
                     /gene="hyfH"
                     /locus_tag="POB34_002621"
     CDS             complement(3699..4244)
                     /gene="hyfH"
                     /locus_tag="POB34_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HDI5919543.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            complement(4254..5921)
                     /gene="hyfG"
                     /locus_tag="POB34_002622"
     CDS             complement(4254..5921)
                     /gene="hyfG"
                     /locus_tag="POB34_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HDI5919544.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAELDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFD
                     HPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSY
                     KPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAP
                     LIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK"
     gene            complement(5911..7491)
                     /gene="hyfF"
                     /locus_tag="POB34_002623"
     CDS             complement(5911..7491)
                     /gene="hyfF"
                     /locus_tag="POB34_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HDI5919545.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLEPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7496..8146)
                     /gene="hyfE"
                     /locus_tag="POB34_002624"
     CDS             complement(7496..8146)
                     /gene="hyfE"
                     /locus_tag="POB34_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HDI5919546.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILQQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIAIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8158..9389)
                     /locus_tag="POB34_002625"
                     /pseudo
     CDS             complement(8158..9389)
                     /locus_tag="POB34_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
     gene            complement(9406..10353)
                     /gene="hyfC"
                     /locus_tag="POB34_002626"
     CDS             complement(9406..10353)
                     /gene="hyfC"
                     /locus_tag="POB34_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HDI5919547.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMASLFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10364..12381)
                     /gene="hyfB"
                     /locus_tag="POB34_002627"
                     /pseudo
     CDS             complement(10364..12381)
                     /gene="hyfB"
                     /locus_tag="POB34_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(12381..12998)
                     /gene="hyfA"
                     /locus_tag="POB34_002628"
     CDS             complement(12381..12998)
                     /gene="hyfA"
                     /locus_tag="POB34_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HDI5919548.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13251..13721)
                     /gene="bcp"
                     /locus_tag="POB34_002629"
     CDS             complement(13251..13721)
                     /gene="bcp"
                     /locus_tag="POB34_002629"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDI5919549.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(13721..14293)
                     /locus_tag="POB34_002630"
     CDS             complement(13721..14293)
                     /locus_tag="POB34_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HDI5919550.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14439..15317
                     /gene="dapA"
                     /locus_tag="POB34_002631"
     CDS             14439..15317
                     /gene="dapA"
                     /locus_tag="POB34_002631"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDI5919551.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15334..16368
                     /gene="bamC"
                     /locus_tag="POB34_002632"
     CDS             15334..16368
                     /gene="bamC"
                     /locus_tag="POB34_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HDI5919552.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            16581..17294
                     /gene="purC"
                     /locus_tag="POB34_002633"
     CDS             16581..17294
                     /gene="purC"
                     /locus_tag="POB34_002633"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDI5919553.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17448..17675
                     /gene="vapB"
                     /locus_tag="POB34_002634"
     CDS             17448..17675
                     /gene="vapB"
                     /locus_tag="POB34_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HDI5919554.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            17675..18073
                     /gene="vapC"
                     /locus_tag="POB34_002635"
     CDS             17675..18073
                     /gene="vapC"
                     /locus_tag="POB34_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HDI5919555.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            18220..19083
                     /locus_tag="POB34_002636"
     CDS             18220..19083
                     /locus_tag="POB34_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HDI5919556.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLLVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            19098..>21080
                     /gene="tmcA"
                     /locus_tag="POB34_002637"
     CDS             19098..>21080
                     /gene="tmcA"
                     /locus_tag="POB34_002637"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HDI5919557.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHHFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPQTPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLMRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLR"
BASE COUNT         5285 a         5562 c         5666 g         4567 t
ORIGIN      
        1 atgatgcatt ttgccctcgg cgcagtgtaa aaccgcgact ccccacatcg cgttttcact
       61 catcaccagc ccggaaaacc agattaacaa actgaaacag gcaatacctg ccagattacc
      121 gcaggccacc agaagtgcgt taatcagcca agttcgccaa ctaataacgc cccgactttt
      181 tgccattacc gtcattaccg acgaggtgaa caggttggtg ccgcaaaccg ccagcaaaat
      241 aaagccaagt gtaaagcaaa ggccgcccac cagatgggtt aatgcctgtg aagacggggc
      301 atcggcgata actgaaaggt aaaaagtaaa tccgatcgcc ataaacacgc cagcggacat
      361 cgccattaaa aagaccgaca ctttactgcg ggcaatttta tgggcggcaa tccgttcagc
      421 gattgccgct tttctggcgc tcaactgcaa gtcgaaagag agtttgtttc tcatgcatac
      481 tcctgcaaaa gcagattaca acacctcgcg aaccgagatt cccagacgct gcattcgtga
      541 cagcagcgtg gtgcgcttca tccctaatcg tgtcgccgcg ccacggggcc cggcaacaat
      601 gccattggtt tctcgcaata cctgaacaat gcgctgacgt tcttcttcgt cattctctgg
      661 cgtcgtcggg tgcagcaact gcgccattga actgcgaagc gctgaatctt catttagcgt
      721 cggcagtaaa cggctttgtc ggacattaag atgtaaattc agactgttac cgcgagtcag
      781 cagtaccgct cgctcaatca cgttttccag ctcgcgcacg ttgcccggcc agtcccacga
      841 catcaactgg cgtagcgcct cggtcgggat ggcgtcaatt gaacgattca tatggcgcgc
      901 cattttttgc gtgaaatgct ttgctaaaag agggatatct tccggacggt cgcgcagcgg
      961 cggcaattcc agtgggaaga cattcaggcg ataaaagaga tcgctgcgaa actggcgatc
     1021 ttcaaccatt tgccacaaat cacggttggt ggcggcaatg actctgacat ttaccgggat
     1081 cgttctactc ccgccgagac gctcaatctc ccgttcctgc aatacgcgca gcagtttagg
     1141 ctgaagttct aacggcagat cgccaatttc atcgagaaac aacgtgccgc catcggcaat
     1201 ttcaaaacga ccacgatggg tattaatcgc accagtaaac gcccctttgt catgaccgaa
     1261 taactcactt tccagaagac tggcggggat ggcagcgcag ttgattttga ccagcggctt
     1321 gtcgcgtcgc gggctaagtt gatggatcgc tctggcgatc acctctttgc cggttccggt
     1381 ttcaccgcaa atcaacaccg tactgtcgct cttcgccaca atatctacct gctggagtag
     1441 gtcttccatt gcctggcttt gatagataat gtcaccgacg cccagattcg aaaggagctg
     1501 ctcgctaagc tggtggtttt cttgctgcaa actttcctgc aaatcggtca tgctacgcca
     1561 ggcatcggca ttgccaacgg caatagcgat gcgatcggct atgtgttgta gtagctggca
     1621 gttttcctca ctaaagagag tggaagaggt atgcgccagt aacaatgcac ccggtgtatg
     1681 gttgccaaag gtaagcggta tcaggagcgc agattcgcag ccgttagatg caagtaagag
     1741 taataacggg tcgcgttgcc agagaaacag atcgtctgca cggtgcgtca aggtcggttg
     1801 tcgggtttgt agcgtttgtg tcagcaatac actgtcgcca ggcatattgc gtggcagaca
     1861 cgcacaatgt gaggcagaaa gatcgctgca ccacaggctg aacttctcgt tctttcgatg
     1921 atcgcccagt accatactga ctgaagccag accgaaaaaa tgatggatct cacgagcgac
     1981 gtcagcgatc agatcgtcga gatcaagatg tgacagcact gcattagtga tatccaccag
     2041 tacccgatga tgatcgcgat ctttctccag caaacgcagc gccacctgtt gttcttcatc
     2101 attgagccat gattgcagaa ccggggtgat aatgcgtgcc agccagttaa acagtgtgca
     2161 atcctgggga gtaaaacggc tgccgtgggt acgaataaag cgtagctccg ccattccact
     2221 actgccgaga aggacggcgt gacgcgaata atcgaccatc tcgccctgat actctactgt
     2281 tgccgtcaca tcgctgccat catcatggta gcggtaatag aagttgcttt tgagaacatg
     2341 cagttcaata agttgtaccg ggctgaacgg cggcggcagc gggatgaagg cgcgtaataa
     2401 agacgccttg ccgtgtttct gtgctaaccg ctccgcgagc attccgttta ccgcttcaat
     2461 tgttattcct tgtggcgggg caaacatcgc ctcgtctgac atagccatca acagtcctta
     2521 ttaatgagaa atccttctca cgcaattttt ctggccatca gatagatggc cggttcctgc
     2581 acaatttcat ccaacagatg aatgaattga atgctacgcg cctgccacac agacgtcgcg
     2641 tcatctgcca gtagcgcgaa tgcccgggtc aacagggcgg tgtggctggc gtcaataaca
     2701 atctcaccaa aggtcatcac ccccagcatc ttacgtcggg cttgctcctc ttcgacctgt
     2761 gcaagccaat cttcgtattc cgtcagtggg cagcggaagg cgacattcag acaatcaatc
     2821 acgccaacgt gatgaccgat agccagcgag taatacatta cctgagagct gtgttccggc
     2881 atctcgtcgc tactggcgac aaactttttt cgcagcgtcc agaaaacaat ttccccgcac
     2941 tcctcagcca tgtaaccccc ggagaacgcg aaagagttgc tgaacgatac tattgagacg
     3001 cggatcgtcg gcttcgcgca accaggtgtt aacccgattt cctgtaggat cgtcgcttaa
     3061 atggcgcagg agccgatcgc aaatttctcg cccctgacga tagcccgcca gccgccgcgc
     3121 ttctcgctca atggcgatac gctgtaatgg cgggatctgt ggccacaacg gttgcatagt
     3181 caccccggtg ggatcgcgat aatccacagc gtgaatcttc tgttgcagca aaccgagcgc
     3241 cacggcgaaa ccgtgaatgg tggcggccgg tgttggcggg cagccgggga tccaaacatc
     3301 aatggggaca atggtgtcgc tgccgcccca gacgctgtag agatcgtgga aaataccgcc
     3361 gccgacaccg cacgcgccgt aggaaacaca gattttatga tcgggggcag actcatacgc
     3421 ccgaagtgca ggcatacgca tcgcccgggt gactgcgcca gtaaataaca aaatatcggc
     3481 gtgacgcggt gatgaaacaa ccttaatgcc aaaacgttct gcgtcgaata ctggtgtgat
     3541 ggcagcaaaa atttcgattt cacaggcgtt gcagccgccg caatcgacgc gataaacgta
     3601 agccgagcga cggatatcct gtagcaaatg ctgcttcatc ttttgcgttt gctcgtccag
     3661 cgtgatgggc tggctgacat attgcgtaag cactggactc atagctgctc cttagccacc
     3721 agcggtacat ctgtatcgtc gttgatcagc gtcgcgcgtt gtttgcactc cgggcagacg
     3781 ctcgcttgcg cccgcaacat ttcgcggttt tgtggcgcat tttgttgctg tgccaacaat
     3841 tcagcagcca gtgcgacggt tttttgcggg gcaaacgggc gttcgcaacg gctgcaacgt
     3901 tgtagatgga acgtcgcgcg ggtatagaga tcggctttat tggtgacggt cagttcaaag
     3961 ttattggtaa gctggatggc tctggtcgga cacacttctt cacaacgtcc gcagtaaata
     4021 caacgcccca gatagagctg ccaggtgcgc gaattttgct ggtcgtcggt ctggatagtc
     4081 agcgcatttg ccggacaagc acaggcgcag gcaccgcagg caatacattg gctgggcatc
     4141 aggtccggtt tcccgcgaaa gcctgggctg acctccagtg gcgcgaaggg atatttcacc
     4201 gtcgcggttc cggcgcgcat aatagttttc agtaacttca gcatcttctg tccttacttc
     4261 agcggcgaac ggttacgatc aatgccgtag cgttcgatct ctttatacgg cacggttttt
     4321 gactggcgct tgcgcacatc taccagcgtc acacggtcgg tacaggagta gcagggatca
     4381 aggctaccga taatcagcgg tgcgtcagaa acggtattgc cgcgcagcat gtaacgcaac
     4441 accggccagt tggcgtaggt ggcggcacgg cagcgccagc ggaacaattt ttggttatca
     4501 ccgagcatgc tccagtgcac gtcttcaccg cgtggcgctt caacaaagcc cagcgcgaat
     4561 gcgtgaggtt tatagctaaa gccttcggtc agcagtgggg tatccggcat gttgtcgagg
     4621 gcaaattcca gcattgccag cgaatcaaac gtctctttga cacggaccat cacgcgggag
     4681 aaaacatcgc cgccggtaaa ggtaaacagt gtttttggaa tattgccgta gtcggcgtag
     4741 gggtgatcaa agcgcaaatc acgggcgatt tgtcggtcga gaatgccaat gccctgagtg
     4801 cgttgttcca tattcggcgt agcaagcagc atctctacca gctccgacac gtcggcgcgc
     4861 atctcgcgca ccagtttcag cgtttgcaga cgttgctctt tgagaatatc gcggcgaaca
     4921 ccaccaatca gattcagacc gtaggtttta cgcgacccga tcagcaattc cgccatcgtc
     4981 atcgactttt cccgcacgcg gaaaaattgc ataaagccgg tatcaaaacc aacgaaatgg
     5041 caggagaggc caaggttaag caaatgactg tgtagccgtt cgacttccag cagaatcgag
     5101 cgaatagtat gtgctcgttg cggcacctca atccccagtg cattttcaac cgaattggta
     5161 taggccacac tgtgggcaaa accgcaaatc ccacacacgc ggtccgataa gaaggtcact
     5221 tcgttgtagc ccatccgcgt ttctgccagt ttctccatgc cgcgatggac ataaaacagg
     5281 cggtaatcag catcgacaat ttgctcgcca tccacaaaca agcggaagtg acccggttca
     5341 tcggaggtga tatgcagcgg gccgacaggg atcacccgcg catcgctgtt gccctcattg
     5401 ataaacgcat acgtttcgga atctgtcgtc ggttcagggc gcagtcgata atccatcgca
     5461 tctttgcgca gcggatgcat atcttccggc cagtcatcgg gcaacaccag gcgacgctga
     5521 tccggcaggc caaccggaat cagcccgtac atatcgcgaa tttctcgctc gccccagacc
     5581 gcggcaggga cgcgcggtgt gactgacgga aactcccgac tgtcggcatc gaccagtgcc
     5641 ttcaccacga tccagcattt ttcggcccct tccattgaaa gggcataata aaccgcgtaa
     5701 tgaccgttaa gtgtccgctc gtcgttgcca aacaggactg gaagccagcc atcatgttga
     5761 taataaagat aatgtacaac gtcaggcagc agattgattt tcaccgtaat ggtgacctgt
     5821 tcaggcgttt gtcgctcttc atccagctcc gcgccgggga actgcgtttt cagggcggcg
     5881 agaatcgctt cgccacgatt tgacgatgaa ttaacgttca ctgtgtttct ccgaaacaga
     5941 tgcggtgcct gaaggcaaaa aatcatgcca ggtgctacgt tgtgcaggca ggtcgtgcgt
     6001 ccctgagagg actatagtgg aagcgccagc caggatcctg atgacaggtt gtggaatatg
     6061 cgttcccatc gccagcatca tgaccagcag aatcaccatt ggcgaggtgg tcaaccagcc
     6121 gagatcaccc cggttaacgg cctgcggcgg tttcgccatt aacacccgcg cagccatccg
     6181 taccaggccc gccagcacca gcgttaacag caataacagc aggacgataa tcagcaggtg
     6241 attacgtgcc agtcctgcgg taacggtcat aaattcgcta agaaaaatgt tgaagggcgg
     6301 catccctgcc agcgccagcg caccgccgcc aaacagcacg gcggtaaatg gcatgatttt
     6361 gagcatccca cagacgacgt tgagatcgcg cgtgccgtac ttgagcagta cattgccgga
     6421 accgcagaac agcagcgttt ttgccagact gtggtttaag gtgtgcagca gcgcggcaaa
     6481 aattcccagc ggcccgccaa tgcctagcgc caccgcgacc agccccatgt tctccacgct
     6541 ggagtacgcc agcagacgct taatgtcccg ctgtaccaga atgaaaaatg ccgccacggc
     6601 aaccgacaac atgccgaaga tgagcaacaa ccggttgggg aaatcgctgc cgatggcttg
     6661 gcaaataatg atgtaatagc gaatcagcac caacagcgcg cagttcagca atacggcgga
     6721 gagcagggcg ctgaccggac tcggtgcttc actgtgagca tccggcagcc aggcgtgcat
     6781 gggaaatagc ccggttttgg taccaaagcc aattagcaca aacacaaagg ccaacagcat
     6841 taatgttggt tcaagcaagg acgattgctt aagaacctcg ctccagaata tcgccatttc
     6901 tgcctgcggc ataacgctgg cggcgttggc gtataccagc acggtaccga acagaccaaa
     6961 agcgacacca acagtacaaa taatgatgta cttccatgct gattccagcg atgaacgctg
     7021 accgtaaatg cctaccagaa acgccgagct taaggtggtg gcttcgattg ccgcccacat
     7081 cacaatcagg ttattgctgg taacaaccag cagcatggtg aacaaaaaca gatggaagaa
     7141 accgtagtaa tcgcacagcg taacgggtga aagctcgccg tgtgccactt catgacgcat
     7201 gtagccaatc gagtaaacac cggtgagaaa gccaatcaca ccaagaatgg cgaggaacaa
     7261 accgcccaga ccatcaatat gaagccacag tcccgcagcg aatatttctc ctgcatcagc
     7321 ggcagtttgg accacccaga gtgccagaat cagcagcagt gtgatcccta agctatgtaa
     7381 tacagtcacc gtacaagtcg cagagagccc ccgtttccgg caggcaaaac agagcagcga
     7441 aaaaagcagc ggcgtgagca ggagtaaagc gaacatcaca gaataactca tctcattatc
     7501 ccttcagcgc ggtcaagttg ttcacgtcca gcgtgccgtg ggtacgccat atttttcttg
     7561 ccagtaacac catcacaatg acggcgaaga tggcgtcggt agcgatagct atttccacca
     7621 gttccggtgc tcgccaggca agaagcgcca gcaccagatg ggagccgttt tccatcaggc
     7681 agtaaccaaa aatttgctgc aggatattgc gctggctgac aatgcacagc aggccaagca
     7741 gaaaatgacc taacgctacc gccagcgccg gtttcagccc ggtagccatc ggtagcttca
     7801 cgggctgaac gacaaatgcg caaagcagga caattaacgc cgcgagcagt gccatcattg
     7861 ccggaccgaa taacgctttt tccgggatgt tctggggaat atttcgtgca gcgtaagtca
     7921 tgattaacgg taccagcagc actttggtga taaaggcgct ggcggaccag atcagcagtt
     7981 gctctgcggc gaacaggcac gagagagtgg cgaaaataga caccagcacc agcgactggc
     8041 aggcgtaaaa tccgcatgac aggcgatagc ttttgacgct aatcacaaac agcgatgtca
     8101 gcatcatcag tcccgccaga ttatttacga tcatagaacc ggtcattcat catctcctta
     8161 ctgcaaccag gtcgcggcga ttacgctgga aatcagcgac atcacaatca gtacaatcaa
     8221 caccaggcgc attgaccctg gcagcggtgc ggcatcggcg acggcctcgc tcggtttgcc
     8281 aggcacaacg cgaccaaacc agcgaataaa ccaggcgaaa ctggcgaccg attcaatcat
     8341 cagcagaatc atggcgggca gcaggatcca gtactccact gacaacgcaa aaccggcagc
     8401 aaacagcggg aatttactga agaagccgtt gaacggcggc acaccggtaa tcgccagcgc
     8461 tgccacgcag aaacccacgc ctggcaacgg cagggtgtgc aatacgccac gcagacgcgg
     8521 caacaagcgc gtgccgcagc tgtaactcag cgcacctgct acaaggaaaa acaggctttt
     8581 agcgaacgcg tggttgacga tgtaggcgat gctgccctcc agtgccagcc gcccactttc
     8641 accttcgatg cggcgtggag ataggcactg atcggtgttg gcgcttccat tgcatccggt
     8701 agccacgctt gcatcggtag ctgggccgat ttcccccacg cggcaaacag gatgccgcca
     8761 taaaccagat aacgtgcgtc gccgtgtaac tcgctcatcg cgctaagcgc aaacgttccg
     8821 gtctgcaaaa acagcgtggc ggcggcaaga tacaacccca acgagccgat atgagtgata
     8881 agtaacgctt ttagtgctga acgctgtgct ttatcgctct ggtaataact gatcaacgcc
     8941 caggagcagc cgcccgtaat ttcaaaaaac aacaactgac cgagcagcgt cgaggagagt
     9001 accagtcccg ccatcgcgcc gataaacaac agcaaaaatg cgtaataacg attcgtgccg
     9061 ttatgcgggt gttcgcgatt tttatccgtc agataacccg tggagtagat cgtgaccagc
     9121 aaaccgagaa acaccaccac aaacagaatc agcgtactca cgcgatcaat gaccaggcca
     9181 aacaatgcca catcccccac gttgaccaat gtcaacgtga cggcaacttt atcggcctga
     9241 taaaacgcgg agatcagtga caacatgcac agcgtggtca gcgcggcgaa caaaaccccc
     9301 cattcggcgg cccgacgttg tggcgaaaac gaaacgacca gtgcgccgat aaaaggcagc
     9361 aataacgtcg tcagagcaag attttccata ttccgtcagt gctccttaca gaccggttaa
     9421 ccagaagacc catgcaagcg cagcaaggct gaagccaagc caggtcacat ggtgaatgag
     9481 taaaaaacgc ccgcgtgcca gcgtgttttc tgcgattgag gccagtacaa aaatcagcaa
     9541 aaccttgagc agcgtaacga caagtgaagt cagcaggcag gcgagagaaa gttcttgcgc
     9601 gcgcccaaag ggcagaaaca gggccacaaa cagtgatgcc atcacgacct gtttcagccc
     9661 cagcccccat ttcgccagcg ccagcccggc accggaatat tcggtcagcg ggccttcctg
     9721 taattcctgt tctgcttcag caacatcaaa gggaattttt cccatctcaa tgaagcaggc
     9781 aaaaccacag gccagtaacg ccagtacggt ggttagcggc gagttccagc ccatcgccag
     9841 cgtattgctg atcatctcga tatgcgtgga acctgctatc agcgccaata ccagcagtga
     9901 gagaataagc attggttcga ccagaatgcc gagcgtcaac tcgcgactgg caccgactcc
     9961 cgcaaacgga cttccggtat ccagcccgga aagagcaaag aaaaaacgaa acagggcaag
    10021 aagatagata agggtgatca gatcgccgcc gcccgcaaaa ggggaaacgg taataaacag
    10081 tggtaaggcc atcgccagca ccagcatgct gctgattaat acccacggca tcaggcggaa
    10141 catcagacct gaagatgtcg gcgcaacttc ctggcgttta aacagtttgt ggatatcgcg
    10201 ataatcctgc cagatccccg gcccgcggcg ggagtgcata cgcgcgcgta tctgccggga
    10261 aatacccgta aaaagtgggg ttagcatcag cagaatcacg gcctgtgcta acgcaaaaat
    10321 aaccagatat ccgtcgcaaa gagtttgtct catggtgatt tccttagacg gcaatagcga
    10381 ttagcagcac aaccagtgcg gcgaccacat acaggcaata gagacgaaag tcgccgcttt
    10441 gcagatgctg tatttctttg gccagccgtt gggtggcgct cacgatgggg cggatcaccc
    10501 gctcatccca gaagggttct gtgctctgag ccctggcggt gacgtgcgca aaacctttat
    10561 ttagcctcag cgtagggtcg agctgttgtc gtagacgaaa taacgcagag aagaccacac
    10621 gcagcggctg catcacgcca ttgcctgacg gggccatcgc attttcccag ccgtagccgc
    10681 atgcccaggc atctcctgtg cgacgaaacg ctgcgcggcg cgaacgacac cacagccagt
    10741 aaaggccagg cagcaaaggc atcgccagta gtaacagcaa cagtaatgag ggggtgacct
    10801 gtgtatgaaa cgtgccgggt acaagtgcta ttccgctggc gacagtgacg ggaggggtat
    10861 tggtaaacgc atgggcaata tgcattatct tcggtgccag ccaactggca ctaagcgcaa
    10921 ttaatacgca gagtgccgcg agcagtagca tggcgacgat catcgtattt ggcacttcct
    10981 gtgcctcttc agcgtgtgta ctgcgcggcg caccacagaa agtaataccg tacattttta
    11041 cgaagcacat tactgccagc ccaccagtga ctgccagcat taccatagca ataggacccg
    11101 caagttgtag cgctacggct tccacacgac ttagtgagaa cagcgactgc caggtgtacc
    11161 attcgctgat aaaaccattc agcggaggaa tggctgatat cgcgaggcaa ccaatcaggc
    11221 atgctgcggc tgtccacggc atccgtttcg ccagtgcccc cattttttcc atgtcgtggg
    11281 tatgcaaacg cgaaataatc gcgcccgcgc cgagaaatag cagccctttg aacagcgcat
    11341 ggttgagcag atgaaacagt gcgccgagca gtccaaccac ggtgagcagc gggtcgtgca
    11401 gtgacagacc gaccatcgcc acaccgactg ccagcaaaat aatgccgacg ttttcgacgg
    11461 tactccaggc cagcagccgt ttgatatcct gttccgccag cgcatacagc acgcccagga
    11521 gcgcggagat tgcgccgatc gccatcacca gaatgcccca ccacagcggc aaacccgttt
    11581 gcgccagcag gtccatcgct actttcagga tgccgaaaat accgattttg accattacgc
    11641 cagacatcaa cgccgaagcg tgcgatggtg cggcagggtg agcgcgcggc aaccagctgt
    11701 gcaacggcat catcccggct ttcgcgccaa aaccgaaaaa ggccagcaga aacaccgccg
    11761 acgccagccc cggagaaagt gaaagcgtgc gaaactggca aaatcgaggc tgccgctttc
    11821 gcgccccatc agcaagaagg cgatcataat cagcaccgat ccggcgtggg cgataaaaaa
    11881 gtagagcatg ccagcgttga tcgacgtttt atcctgcctg gcaatgacca gaaaccagga
    11941 agacagcgac atcatttcaa acagcacgat gaaccaaaaa gcgttgtcca tcaccagcag
    12001 ggcaaccatc gatgcgatga aaagattcat aaagaagccc atcgccgccg cgcctttgcc
    12061 ctcgtattcg cgcatataag tcaatgaata gagcgaacaa accaccacca gcaaggagat
    12121 aaccagcacc ataaatgccg agaggctatc cattcgcagg gttaaatcgg caaagtcaaa
    12181 aggggtggca aaatgggcga ctaaagttac accagcatgt aattgcgtaa ttccgctgat
    12241 gatgccaatc acgccgccca ccagcgatgt gatgccggaa agcttaatag ccagtctgtc
    12301 cagaccgagt aaaaacagcg aagccagact ggcaaacaga tagaggatca gcgaccaggt
    12361 taataattgc agggcatcca ttagcgttgc tcctgagtga gggaaagggg atcctccccg
    12421 ccattggcaa accagcttgc ggcaaggcgc tgtttttctt tcatctgtcg ttgcaggcta
    12481 tcgtcggtga tcagccgtaa cgcctgattc gggcaagcgc gaacgcaggc cggaccttct
    12541 ggcaagaaat cacacaggtc gcatttcacc gcgacggtct ggacacccgg ttcccagcgc
    12601 agcaatgcat gttggggcgg cacgttttct tcgccgtctt tgagtgagcc ttcagcctga
    12661 aaaacatatt gcgcaggggc attcaccgga cggcttcctg aagcgctgat tgcgccaaat
    12721 gggcagacca cggcgcaaag cttgcagcca atacagaggc tttcgttgag ttggatcgca
    12781 tcatccctct gggagatggc attaaccggg cagacctgca ggcaaggggc ttcctcacag
    12841 tgatgacaca cgacaggggc agtgagtgtt gacgtcttcg ccagggccag gcgcgggtgt
    12901 tgctgtaaac cttgcgtttt atgcacgtcc gaacaggcag cgagacaggt attacatcct
    12961 gtacaccaca gtggttcggc caccacaaag cggttcatgt cggctccttg aaaatgatat
    13021 gaaacattgc atttcatatt gcataaagga tgccattctg attaactcaa tatattcaat
    13081 aagttatgta ttgactggat tctgaacggt acaaaaatga tggtcattaa cgttcagcaa
    13141 aaaatggatt cgacacccgt gtcgcagggg aaagcgagaa tgtgatcttg atcacgagaa
    13201 gtgaggtatg ctggccgcaa gcgcagccag cacggaatgg agcaaagtaa tcaggcgtgt
    13261 tctttcagcc agttcagcac aacgtcgtgg tgattgctgg ttttgaaatc gtcaaagaca
    13321 tgttcgattt tgccatcagc gtcaatcagg aagctgatgc gatgaatgcc atcgtaggtt
    13381 ttgcccatga aggacttttc accccagacg ccgaattgtt cgcacacctg gtggtcctca
    13441 tcagacagga gcgtaaagtt aagcagctct ttttccgcaa aacgggagag tttttcgggt
    13501 ttatcggtgc tgatacccag cacatcaacg cccgcttttt tcaactcatc catgttatcg
    13561 cgtaagccgc aggcctgtac ggtacagccg ggggtcatgg ctttcgggta gaaataaacc
    13621 agaacacgct gtccctggaa gtcggtcaaa ttaacttgtt ctccgtcttg atccggcaag
    13681 ctaaatttcg gtgcgatatc accggctttc agtggattca ttacttaact ccatcctgtt
    13741 catcatgttg ggaataattg acgacgttaa tactgccttg tgcattgagt tctgtacata
    13801 gggctttgaa cgcttgctca atatttgctg cgtccgcaga tgcggggctg tgggcggtta
    13861 tctgaatatg caactgcgca gcccgttcat tttcagcagg ttgcgtgcgc gacaccagct
    13921 ccgcaatgtt catatgatgc gcgtcgaaaa gtgctgtgaa gcgttcaatt aaatgcgggg
    13981 agtctgccac atcgacctga acccagacag atgctggcat tggcggacgc ggacgcgccg
    14041 tcgtgcgctt catcacgatt aaaagatcca gttcggcacc tttcaacggt aacgttgatt
    14101 caatcagagt aatggcattc catgaaccgg aaagcagcat aataaacgtg aactcttctc
    14161 ccagcatcgc caggcgactg tcttcaatat tacagccgca actactgaca tgacgggtga
    14221 tggtgttcac aattccaggg cgatcggcac ccaacgcagt gatcaccaga taatgttgcg
    14281 atgacagtgt caaactggtt attcctttaa ggggtgagtt gttcttaagg aaagcataaa
    14341 aaaaacatgc atacaacaat cagaacggtt ctgtctgctt gcttttaatg ccataccaaa
    14401 cgtaccattg agacacttgt ttgcacagag gatggcccat gttcacggga agtattgtcg
    14461 cgattgttac tccgatggat gaaaaaggta atgtctgtcg ggctagcttg aaaaaactga
    14521 ttgattatca tgtcgccagc ggtacttcgg cgatcgtttc tgttggcacc actggcgagt
    14581 ccgctacctt aaatcatgac gaacatgctg atgtggtgat gatgacgctg gagctggctg
    14641 atgggcgcat tccggtaatt gccgggaccg gcgctaacgc tactgcggaa gccattagcc
    14701 tgacgcagcg cttcaatgac agtggtatcg tcggctgcct gacggtaacc ccttactaca
    14761 atcgtccgtc gcaagaaggt ttgtatcagc atttcaaagc catcgctgag catactgacc
    14821 tgccgcaaat tctgtataat gtgccgtccc gtactggctg cgatctgctc ccggaaacgg
    14881 tgggccgtct ggcgaaagta aaaaatatta tcggaatcaa agaggcaaca gggaacttaa
    14941 cgcgtgtaaa ccagatcaaa gagctggttt cagatgattt tgttctgctg agcggcgatg
    15001 atgcgagcgc gctggacttc atgcaattgg gcggtcatgg ggttatttcc gttacggcta
    15061 acgtcgcagc gcgtgatatg gcccagatgt gcaaactggc agcagaaggg cattttgccg
    15121 aggcacgcgt tattaatcag cgtctgatgc cattacacaa caaactattt gtcgaaccca
    15181 atccaatccc ggtgaaatgg gcatgtaagg aactgggtct tgtggcgacc gatacgctgc
    15241 gcctgccaat gacaccaatc accgacagtg gtcgtgagac ggtcagagcg gcgcttaagc
    15301 atgccggttt gctgtaaagt ttagggagat ttgatggctt actctgttca aaagtcgcgc
    15361 ctggcaaagg ttgcgggtgt ttcgcttgtt ttattactcg ctgcctgtag ttctgactca
    15421 cgctataagc gtcaggtcag tggtgatgaa gcctacctgg aagcggcacc gcttgcggag
    15481 cttcatgccc cggctggaat gattttgccg gtgacctccg gtgattatgc aatcccggtg
    15541 accaacggta gtggtgctgt cggtaaggcg ctggacattc gtccaccagc ccagccgctg
    15601 gcactggttt ctggcgcgcg tacccagttc acgggcgata ccgcttcatt gctggtggaa
    15661 aatggtcgtg gcaatactct gtggccgcag gtggttagcg tgctgcaggc gaaaaactac
    15721 accatcaccc aacgtgatga tgctggtcag acactgacca ccgattgggt acaatggaac
    15781 cgtctggacg aagacgagca gtatcgtggt cgttatcaaa tctctgttaa gccgcagggt
    15841 tatcagcagg cggttacggt taaactgctg aacctggaac aggcgggcaa accggttgca
    15901 gacgcggctt ccatgcagcg ttacagcacg gagatgatga acgttatttc cgccggtctg
    15961 gataaatctg ccactgacgc cgcgaacgct gcgcaaaatc gtgcctccac cactatggac
    16021 gtacaaagtg cagctgatga caccggttta ccaatgctgg tcgtacgcgg gccgttcaat
    16081 gtggtttggc aacgtctgcc agcggcgctg gaaaaagtgg gcatgaaagt gaccgacagc
    16141 acccgttcgc agggcaacat ggccgtaact tataagccgc tgtctgacag cgactggcag
    16201 gaactgggcg cgagcgatcc aggcctggca tccggtgact ataaactgca ggttggcgat
    16261 ttagataacc gcagcagcct acagttcatc gatccgaaag gtcatactct gactcagagt
    16321 cagaacgacg cgctggtagc tgtcttccag gctgcgttta gcaagtaaaa atacagggct
    16381 ggaatcatcc ggcccttttt tctgatatga tacgcaaacg tgtgcgtcgg caggaaaacg
    16441 cgattttagc ggtaattcgc acgaaatttg tttgtcggac gtagttcgga taaggcgttc
    16501 acgccgcatc cgacaaaaca tccggcacac cagacagcaa aagattttaa aacgttaatt
    16561 cacacccagg agtgataaag atgcaaaagc aagctgagtt gtatcgtggt aaagcgaaaa
    16621 ccgtatacag cacggaaaac ccggacctgt tggtgctcga attccgcaat gatacgtcag
    16681 caggggatgg cgcgcgcatt gagcagtttg atcgcaaagg tatggtgaac aacaagttca
    16741 attacttcat tatgagcaaa ctggctgaag cgggtatccc gactcaaatg gagcgtctgc
    16801 tctccgatac cgaatgtctg gtgaaaaagc tggatatggt gccggttgag tgtgtcgtgc
    16861 gtaaccgtgc tgctggctct ctggtgaaac gtcttggaat cgaagaaggt attgagctga
    16921 acccgccgct gttcgatctg ttcctgaaaa acgacgccat gcacgatccg atggtcaacg
    16981 aatcttactg cgaaaccttt ggctgggtga gcaaagagaa cctggcgcgt atgaaagagc
    17041 tgacctacaa agcgaacgac gtgctgaaaa aactgttcga tgatgctggt ctgattctgg
    17101 tcgacttcaa gctggagttt ggcctgtaca aaggtgaagt ggtactgggc gatgagttct
    17161 ccccggacgg cagccgcctg tgggacaaag aaacactgga gaaaatggac aaagaccgtt
    17221 tccgccagag cctcggtggc ctgatcgaag cctatgaagc cgtcgcccgc cgcctgggtg
    17281 tacagctgga ctgatttttc tggttgcttg tttctttagc cggagggtac tccggctaaa
    17341 tgtctttgat acccccgcta caatacttct cattatctcc ctttgacagg aaagtcatac
    17401 caggccaata tgtatataca taacgtatat cccaaaaagg gggggctatg gaaactactg
    17461 tatttttaag taatcgcagt caggccgtca gattaccgaa agcggtcgct ctgccagaag
    17521 acgttaagaa agtagaaatt attgcgattg gtcgtacaag aatcatcact cccgccggtg
    17581 agtcctggga cagttggttt gatggcgaaa atgtgagtgc ggattttatg gatatccgtg
    17641 atcaacccgc aatgcaggaa agggaatcat tttgatgctg aaattcatgc tggataccaa
    17701 tatctgtatt ttcactatta agaataaacc agtacatgtc agagagcggt ttaacctgaa
    17761 ttctggccgg atgtgtataa gttctgtaac attgatggag cttatctatg gggcagaaaa
    17821 aagtcagatg ccagaacgca atttggcggt tattgaaggt ttcgtatctc gtttggtagt
    17881 gcttgattat gatagtgctg ctgcgacgca taccgggcaa atcagggccg agcttgcccg
    17941 gcagggaagg cctgttggac cttttgacca gatgatagcg ggacatgctc gcagtcgcgg
    18001 tttgattgtg gttaccaaca atacccgaga gtttgagcga gtggcgggaa tcagactaga
    18061 agactggagc taacattctc ttttacttct aacattcgta aattatataa cgtggcatga
    18121 agccttaatt cctccgccat ttcatcccgc tttcctgtgg taatcttcct ctgaaccacc
    18181 tacgatcatt tctataatga atatattgtt gaggtatcta tgcgttggca agggcgacgt
    18241 gaaagtgaca atgttgaaga caggcgcaac agctccggtg gtccgtctat gggcggtcct
    18301 ggttttcgcc taccaagcgg taaaggcggg ctgattttac tgttagtcgt gctggttgca
    18361 ggctactatg gtgttgattt aaccgggtta atgactggac agccggtttc ccaacaacaa
    18421 tcaacgcggt ccattagccc taatgaagac gaagctgcaa aattcacctc ggtgattctg
    18481 gcaaccacgg aagacacctg gggacaacag ttcgagaaga tgggtaagac ctatcagcaa
    18541 ccgaagctgg tcatgtaccg tggaatgacg cgcaccggct gcggggctgg tcagtccatt
    18601 atggggccgt tctattgccc ggcggatggc acagtttata tcgatctctc cttctatgat
    18661 gacatgaaag acaaacttgg cgcggatggc gattttgccc aggggtacgt tatcgcccat
    18721 gaagtcggtc atcatgtgca gaaactgtta ggcatcgagc cgaaagttcg tcaactgcaa
    18781 caaaacgcga cgcaggcgga agtgaaccgc ttatctgtgc gtatggaact ccaggccgac
    18841 tgttttgccg gtgtctgggg gcataacatg cagcagcaag gcgttctgga aaccggcgat
    18901 ctggaagagg cgctgaacgc ggcgcaggcc atcggcgatg accgtttaca acagcaaagt
    18961 caggggcgag tagtaccaga tagtttcaca catggcactt ctcagcaacg ctacagctgg
    19021 tttaaacgtg gtttcgacag cggcgatccg gcacaatgca atacttttgg taaaagcatt
    19081 taacttccgg ggcagggatg gctgaactga ctgcgcttca cacattaaca gcgcaaatga
    19141 aacgtgaagg gatccgccgc ttgctggtgt tgagcgggga agagggttgg tgttttgatc
    19201 atgcgcttaa gttgcgtgat gccttacctg gcgactggct gtggatttcg ccgcagccag
    19261 atgctgaaaa ccactgttca ccctcggcgc tacaaacttt acttgggcgc gagttccggc
    19321 atgcggtatt cgacgcccgc cacggctttg atgccgctgc ctttgcagca cttagcggaa
    19381 cgttgaaagc gggaagctgg ctggttttgt tactccctgt atgggaagag tgggaaaacc
    19441 aacctgatgc cgactcgctg cgctggagtg attgccctga ccctattgcg acgccgcatt
    19501 ttgtccagca tctcaaacgc gtacttacgg cggataacga cgctatcctc tggcggcaaa
    19561 accagccgtt ctcgttggcg cattttactc cccgtactga ctggcacccc gcgaccggcg
    19621 caccacaacc agaacaacag caactcttac agcagctact gaccatgccg ccgggcgtgg
    19681 cagcggtaac ggctgcgcgt gggcgcggta agtcggcgct ggcagggcaa ctcatttctc
    19741 gtattgcggg cagtgcgatt gtcaccgcgc ccgcaaaagc ggcaacggat gtactggcac
    19801 aatttgcggg cgagaagttt cgctttattg cgccggatgc cttgttagcc agcgatgagc
    19861 aagccgactg gctggtggtc gatgaagccg cagccatacc tgcgccgttg ttgcatcaac
    19921 tggtatcgcg ttttcctcga acgttgttaa ccactacggt gcagggctac gaaggcaccg
    19981 gacgtggttt tttgctgaaa ttttgcgctc gctttccgca tttacaccat tttgaactgc
    20041 aacagccgat ccgctgggcg cagggatgcc cgctggaaaa aatggttagt gaggcactgg
    20101 tttttgacga tgaaaacttc acccatacac cacaaggcaa tattgtcatt tccgcatttg
    20161 aacagacgtt atggcgaagc gagccacaaa cgccgttaaa ggtttatcag ctattgtctg
    20221 gcgcgcacta ccggacctcg ccgctggatt tacgccggat gatggatgca ccagggcaac
    20281 attttttaca ggcggctggc gaaaacgaga ttgccggagc gctgtggctg gtggatgagg
    20341 ggggattatc tcaacaactc agtcaggcgg tatgggcagg ttttcgtcgc ccgcggggta
    20401 atctggtggc ccagtcgctg gcggcgcacg gcagcaatcc actggcggcg acattgcgcg
    20461 gacggcgggt cagccggatc gcagttcatc cggcgcgtca gcgcgaaggc gttgggcaac
    20521 agctcattgc cagcgctttg caatataggc ctggcctcga ctatctttcg gtgagttttg
    20581 gttacaccgg ggagttatgg cgtttctggc aacgctgcgg ttttgtgctg gtgcgaatgg
    20641 gtaatcatcg tgaagccagc agcggttgct atacggcgat ggcgctgtta ccgatgagtg
    20701 atgcgggtaa acagctggct gaacgtgagc attaccgttt acgtcgcgat gcgcaagctc
    20761 tcgcgcagtg gaatggcgaa acacttcctg ttgatccact aaacgatgcc atcctttctg
    20821 acgacgactg gcttgaactg gccggttttg cttttgctca tcgtccgcta ttaacttcgt
    20881 taggttgctt aatgcgtctg ttacaaacca gcgaactggc attaccggcg ctgcgtgggc
    20941 gtttacagaa aaacgtcagt gacgcgcagt tatgtaccac acttaaactt tcaggccgca
    21001 agatgttact ggtccgtcag cgggaagagg ccgcgcaggc gctgttcgca cttaatgatg
    21061 ttcgcactga gcgtctgcgc
//
LOCUS       DAMZET010000071        20820 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.071, whole genome shotgun sequence.
ACCESSION   DAMZET010000071 DAMZET010000000
VERSION     DAMZET010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.071"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            4..80
                     /locus_tag="POB34_002638"
     tRNA            4..80
                     /locus_tag="POB34_002638"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38..40,aa:Met,seq:cat)
     gene            complement(155..1408)
                     /gene="amiC"
                     /locus_tag="POB34_002639"
     CDS             complement(155..1408)
                     /gene="amiC"
                     /locus_tag="POB34_002639"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HDI5919558.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1640..2971
                     /gene="argA"
                     /locus_tag="POB34_002640"
     CDS             1640..2971
                     /gene="argA"
                     /locus_tag="POB34_002640"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HDI5919559.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERITAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(3033..4859)
                     /gene="recD"
                     /locus_tag="POB34_002641"
     CDS             complement(3033..4859)
                     /gene="recD"
                     /locus_tag="POB34_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HDI5919560.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4859..8401)
                     /gene="recB"
                     /locus_tag="POB34_002642"
     CDS             complement(4859..8401)
                     /gene="recB"
                     /locus_tag="POB34_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HDI5919561.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIALVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIEQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDVVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8394..11282)
                     /gene="ptrA"
                     /locus_tag="POB34_002643"
     CDS             complement(8394..11282)
                     /gene="ptrA"
                     /locus_tag="POB34_002643"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HDI5919562.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11458..14826)
                     /gene="recC"
                     /locus_tag="POB34_002644"
     CDS             complement(11458..14826)
                     /gene="recC"
                     /locus_tag="POB34_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HDI5919563.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKSHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14839..15162)
                     /locus_tag="POB34_002645"
     CDS             complement(14839..15162)
                     /locus_tag="POB34_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDI5919564.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15147..15554)
                     /locus_tag="POB34_002646"
     CDS             complement(15147..15554)
                     /locus_tag="POB34_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HDI5919565.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(15551..16114)
                     /locus_tag="POB34_002647"
     CDS             complement(15551..16114)
                     /locus_tag="POB34_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDI5919566.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16105..16575)
                     /locus_tag="POB34_002648"
     CDS             complement(16105..16575)
                     /locus_tag="POB34_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDI5919567.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(16760..17554)
                     /gene="thyA"
                     /locus_tag="POB34_002649"
     CDS             complement(16760..17554)
                     /gene="thyA"
                     /locus_tag="POB34_002649"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDI5919568.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17561..18436)
                     /gene="lgt"
                     /locus_tag="POB34_002650"
     CDS             complement(17561..18436)
                     /gene="lgt"
                     /locus_tag="POB34_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDI5919569.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18587..>20820)
                     /gene="ptsP"
                     /locus_tag="POB34_002651"
     CDS             complement(18587..>20820)
                     /gene="ptsP"
                     /locus_tag="POB34_002651"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDI5919570.1"
                     /translation="REIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLADHDRRC
                     YYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSVKEERF
                     RAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLTALFGQ
                     YRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEEAANEF
                     RRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRDLFAEVDKGSVAEWAVKTVIEKFAE
                     QFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATTLAELP
                     QDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGELLVDP
                     EPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEKLGSRI
                     DGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQLPYMPI
                     SEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVDEARRL
                     IERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQYILAV
                     DRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILIGLGYR
                     HLSMNGRSVARVKYLLRRIDYADAENLAQRSLEAQLATEVRHQVAAFMERRGMGGLIR
                     GGL"
BASE COUNT         4932 a         5829 c         5232 g         4827 t
ORIGIN      
        1 tgacgcgggg tggagcagcc tggtagctcg tcgggctcat aacccgaagg tcgtcggttc
       61 aaatccggcc cccgcaacca attattgaac accctaacgg gtgttttttt gtttctgctc
      121 tcccataaaa aaagcgccat tcagcgcctt tttatcatcc ccttctcgcc agcgtcgccc
      181 catcggcaaa atacgcttta attcccgcaa gaatagactc cgcaacttcc tgctggaaag
      241 tcgccgtttt cagtttacgc tcttcctcaa cgttactgat aaacgccgtt tcgaccagaa
      301 tggagggaat atcaggcgct ttcagcacgg caaacccggc ctgttcaacc tgatttttat
      361 gcagcttgtt gattttaccg agcttattca gcaccgcttt accaaactta aggctgtcgg
      421 caatggtcag cgactgcacc atatcgaaca tggtgtggtc gacatagcgg tcaccgcttt
      481 tgctcacgcc accaatcaag tccgaggcgt tctgggtctg tgccagatat tttgccgcag
      541 tactggttgc gcctttggtt gagagcgcaa acactgagga accgctcggc tggcgactgg
      601 taaaggcgtc ggcatggata gagacaaaca ggtcggcacg ctgtttctgg gcttttgcta
      661 cgcgcacttg caacggaatg aagatgtctt cattgcgcgt catgtacacc ttcatattgc
      721 cctctttctc gatcagagag cgcagacggc gagctatttg caataccacg tctttttcgc
      781 gcgttttgta tttccccacc gcaccggagt cttcgccgcc gtggccaggg tcaagcataa
      841 taacaatagg acgatcgcgc cctgctttac ccggttgtgg accactttgt gctggcggca
      901 cctgcttttc gaggtcgcct ttgttgtaat cctccagcag cgccagcagc gggtcctgca
      961 tatcctgtgc attggccgga tagaggtcca tcaccagacg ctctttaaac ccggcgaccg
     1021 gcgcaagggc aaacagctgc ggttttacgt tttgctttaa ttcaaaaacc atccgtacgg
     1081 tctgcgggtc aaattgcccg acgcgcgccg acttgatgaa cgggtcgtct gcacgaattt
     1141 gtgccgccat ccccttgagt accgagttca ggtttacatc ttcgatatcc accaccacgc
     1201 gttccgggtt actcaacgcg aactgcttat atttcagctg acgatttgat tctaccgtca
     1261 cgcgggtgta gctggacgca ggccagacgc gcaccgccac gacctggcta accgcagcca
     1321 ggctgacctg acttacgctc aatagccaca tagcgcccgc gccttgcagt aaacgacgac
     1381 ggctgattgc agtgttggat cctgacatgc ctctcccgag caaaagaaat ctaagctgtg
     1441 taacaagtaa acgactaatt tgaccggaaa ctttagcgaa agacgcataa tctgtcatct
     1501 aataaacggt aaacattctt tttatattca cggcattact gataaaaaag tcgctctcgc
     1561 ataaaattta cacttgcacc ctgcgaaaaa acagaataaa aatacaataa tttcgaataa
     1621 taatgcaaag gggtgtgccg tggtaaagga acgtaaaacc gagttggtcg agggattccg
     1681 ccattcggtt ccctatatca atacccaccg gggaaaaacg tttgtcatca tgctcggcgg
     1741 tgaagccatt gagcatgaga atttctccag tatcgttaat gatatcgggt tgttgcacag
     1801 cctcggcatc cgtctggtgg tggtctatgg cgcacgtccg cagatcgacg caaatctggc
     1861 tgcgcatcac cacgaaccgc tgtatcacaa gaatatacgt gtgaccgacg ccaaaacact
     1921 ggaactggtg aagcaggctg cgggaacatt gcaactggat attactgctc gcctgtcgat
     1981 gagtctcaat aacacgccgc tacagggcgc gcatatcaac gtcgttagtg gcaattttat
     2041 tattgcccag ccgctgggcg tcgatgacgg cgtggattac tgccatagcg ggcgtatccg
     2101 gcggattgat gaagacgcga tccatcgtca actggacagc ggtgcaatag tgctaatggg
     2161 gccggtcgct gtttcagtca ctggcgagag ctttaatctg acctcggaag agattgccac
     2221 tcaactagcc atcaaactga aagctgaaaa gatgattggt ttttgctctt cccagggcgt
     2281 tactaatgac gacggtgata ttgtctcaga acttttccct aacgaagcgc aagcgcgggt
     2341 agaagcccag gaagagaaag gcgattacaa ctccggtacg gtgcgctttt tgcgtggcgc
     2401 agtgaaagcc tgccgcagcg gcgtgcgtcg ctgtcattta atcagttatc aggaagatgg
     2461 cgcgctgttg caagagttgt tctcacgcga cggtatcggt acgcagattg tgatggaaag
     2521 cgccgagcag attcgtcgcg caacaatcaa cgatattggc ggtattctgg agttgattcg
     2581 cccgctggag cagcaaggta ttctggtgcg ccgttctcgc gagcagctgg agatggaaat
     2641 cgacaaattc accattattc agcgcgataa cacgactatt gcctgcgccg cgctctatcc
     2701 gttcccggaa gagaagattg gggaaatggc ctgtgtggca gttcacccgg attaccgcag
     2761 ttcatcacgg ggcgaggttc tgctggaacg cattaccgct caggcgaagc agagcggctt
     2821 aagcaaattg tttgtgctga ccacgcgcag tattcactgg ttccaggaac gtggatttac
     2881 cccggtggat attgatttac tgcccgagag caaaaagcag ttgtacaact accagcgtaa
     2941 atccaaagtg ttgatggcgg atttagggta agcgatgaaa attgtcggat gcgacatgcg
     3001 taacactcgt gcgtcgcatc cggcaattac gtttattccc gcgaactaaa caacgccgcc
     3061 agaccactgc gccgctcagt acgagtggcg attgccgcac ttaatatacg ctcatcggca
     3121 tacagcgaca gacggcgacg cgcgcgggtc accgcggtgt ataccagctc tcgcgttact
     3181 accggcgtgc gttggctcgg taaaatcaac gccgcatggt cgaactccga tccctgcgat
     3241 ttatgtaccg tcatcgccca cgtagtttcg tgctctggca agcgactcgg ttgcacagac
     3301 ttaatattgc cgtccggcat cgcaaaccag acgcgcgtcc cctgcccgcg atccagcgca
     3361 ataccgatat cgccattaaa caacccaagc gcgctgtcat tacgggcaat cattaccggt
     3421 cgaccttcgt accaacgaga gtgcggatga cgatgaattt tgcgcttctg ttgcataaac
     3481 tgctcaattc gctcattcag tccagccacg ccaaacggcc cttcccgcag ggcgcacaaa
     3541 agttggtact cattgaacgc ctgaatgatt aaatccggct cggcacgcgc ttgcagcaga
     3601 tccagataac gtccataacc cgcaagagct tcctcaagca tcgcaatata atcttctccg
     3661 ctctgtaaaa gccgtttttc gatatcagta aaatcctgct gaaaaacggt tttcactgcc
     3721 gttttatcac cacggttgat cgccgcagct aactgaccaa tgccagaatc actgccgaaa
     3781 cgatagcttt tttgcagcag gcagagactg tcgcgcaaag atgccgcttc tgtgccagtt
     3841 cctgccggaa cgtgagttcc cgtcaggcgg cttagctgcc cggcacgctc ggcggtaaag
     3901 ccggcattgg cataagcgca gatatcgccc agcacagccc cagcctcaac cgaggccagt
     3961 tgatcacgat cgccgagaaa gatcactcgc gcatgatcgg gcaaggcgtc gatcagtctc
     4021 gacatcatag gcagatcgat cattgacgct tcatctacca ccagcacatc aagatgcagc
     4081 gggttaccgg catgatgacg taaacgctgg ctacccggct gcgcacccag caatcggtgc
     4141 aaagtactgg catcttccgg aatgcgtttc ttttgttcat cggtcagcgg taactgtcgc
     4201 aaagccttgc cgagagattc ggttaagcgc gcggcagctt tacccgttgg tgcagccaga
     4261 cggatacggc agcgttcgcc gtcggccatt tgaattaacg ctgccagtaa cttcgccacg
     4321 gtggtcgttt taccggtgcc tgggccgccg gaaatcaccg agatccgccg cgtcagcgcc
     4381 actgccgctg caactttttg ccagttaatt tcatcgctta ctggaaaaag tttgtccagg
     4441 gtttgcgcca gtagagcttc atcaacctca atggcatgat tcacctcatt gaagaagcgc
     4501 gctaccgtcc gctcgttaca ccacatgcga ttcaaataaa gacgatcgcc acagaggatc
     4561 atcggcgtgg gttcatctcc cctgctgacc gcttgagaag ccagcaagca ttcttcccaa
     4621 ttttgtagct caccgatttc actgacacag gtcgccaaca gcggatgcga cgcctcgtta
     4681 ttttccagtc gtgaaagcgg caaacagacg tgtccctctc cggcatcatg acttaacagt
     4741 gccgccgcga gggtgacggc aggatgttca tctcccgcca cggtcagggc aaattgcaca
     4801 tccagcgggc gtagctgttt gtgctccaca gcttccagta attgcttttg caatttcatt
     4861 acgcctcctc cagggtcata ccggcaaaca tctcatccat cagggcaatc aacccggcgt
     4921 tgggtcgggt tgtgtaaatc ccttgttgcg gatgttcttt atcaacgcca cgcaggaaca
     4981 gatagataac gccgccaaag tgacgatcat agtcgtaatc agcaatgcga tggcgcagat
     5041 aacgatgcag cgccagggta taaagctgat attgcagatc atagcggtgt gcctgcatcg
     5101 ctgctgccat agcctgttgg gtgtaagccg aactgtcttc acccagccag ttggatttat
     5161 agtcgagcag gtaataacgc ccttcgtggc ggaataccag gtcgataaag ccttttaaca
     5221 tgccgcgtac ctgcatgaac tccagcggcg ggcagccagc ggatagcggg tcaaactggc
     5281 gaattagtgc atcaagctgg ctggcgatga gcggttcgct aatcggcagg taaaactcca
     5341 tctccacctg tttatcgcgg tcggaaagct gactcaggct aacacccgtt tcattgagag
     5401 gtgcctggag gacagccgtg atccactcgg tcaacaccgg ttcccactgc gattcaaagc
     5461 cgccgagttc cagtttttcc tgtacccagt tcgggtcaac cggctgggta aaatcgaggt
     5521 cttcaaacaa actgtgcaag aatgtccccg gtgacgcacc gcgcgggaac tgatgcggtg
     5581 ttaacgtcgg ttcttcaacg acgctgacca cgcctgcggc atcaacatcc agccgtggca
     5641 tcagatcctg agcgataccg tgaccacgct gctgcaagcc ggagtagctg gtgacacgcc
     5701 agttatcgcc gggcaatcgt tgtagtgtcc tcgcattcag ttctgcagtt aaagcatcat
     5761 taacctgcca gggctggtta tcaccagttt gtgccgtttg ccaggcaata tcatcatcgc
     5821 ataacgcttc aatacaggtg cgaagccctg ccgcatcttg cggttcccct ttttgcagca
     5881 aacgcccgag cgcactttgg tggacgtcgg tgtcaccttt tttatcgcca cgacggcgca
     5941 ccagcggtgc aacgccgaga ctgcaatgcc aaaccgaacg cgtcagcgcc acgtaaagca
     6001 aacgcagatc ttccgccaga cgttcgacct ccgcgaggtc gacgctttct ggcgcagcgt
     6061 taagatccaa aactgcctca aacgagtggc gatcgtgata aaacgcctga tcctggacgc
     6121 ggaaattggt gataaacggc agccagacca atggatattc cagccctttc gatttgtgga
     6181 tcgtgacaat ctgcaccaga tgtttatcac tttcgagacg catttgttgg ctggaggcat
     6241 tactgtctgg ctcgaggata tgttgcgata accagcgtac cagcgcatgt tcactttcca
     6301 gctgcgttcc ggcttcttgt agcagttcgc tgatatgcaa gatatcggta agacgccgct
     6361 caccgcctgc cgttgccagc aagttttcag caatgttacg cgccgacatc agcgcccgca
     6421 gcatcggcat aacgccacgt ttgcgccaga tttgccgata accatcgaac tcttcgacta
     6481 ccacatccca cgcatgttcg tcattgttca gcgtttcgat atccagcgcg ttcagcccca
     6541 tcattgacgt tgccagcgca ctacgcaggg tgttctcacg ttcgggcgtc atcaccgcct
     6601 gcaacaacca aagcatttcc tgcgcttcca gagtttcaaa aacactgtcg cggttcgaaa
     6661 ggtaaacgga agggatttcc agcaacgtta aggcgtcgcg cacctgggcg gcctcctggc
     6721 ggctgcgcac cagcacactg atgtccgaag cacgcaccgg acgcgcgtcg tcgccgttca
     6781 tcagcaacgc ttcgccccgc tgtccggctt gtagccagtc gcggatttgc gcagcacata
     6841 cctgcgccat ggtactttga tagtcgccaa cgccgcagct ttcgccttcc atcagccaca
     6901 ttttcatcgc aggctgcgtt tcacctttaa acacaaaacg taacgcctga tttttcccgg
     6961 ctgatttcac tggaataaac ggtatttcgc gaaacatgaa cgcgtcatca gtctggctga
     7021 aaagcttgtt cacactgttc accattcctg gtgcggaacg ccagttggtg tctaaagtgt
     7081 agtgggcgtg aacttcgcta cgcgccttca tataagtgaa gatatccgca ccccggaatg
     7141 catatatggc ctgtttcggg tcgccaatca gcaacaatgc ggtttccggc tgatggtgcc
     7201 agatacggcg aaaaattcgg tactgctggg ggtcggtatc ctgaaattca tcaatcattg
     7261 ccaccgggaa tcgcgtacgg atcgccgctg ccaacacctc gccgctttcg ctacgcagcg
     7321 cggaatcgag ccgacttaac atgtcatcaa aacccaattc gccacggcgg cgtttttcac
     7381 gcgctactgt ttcgcggatc tcagccaatg cgcgggttat caccagatca cggatcgaca
     7441 atggttctgc aagcagttgt tcgatcgcct caaacagtgg atgtcgcggg gtttcccccc
     7501 cggctttcgt gcgatcttct aagaaacgct gagagaattt ttccagcgac tccggtaact
     7561 gataactgtt ggtctcttct tctgcccagg cgctgatctt ctcgatccat ttagcctgat
     7621 tgctacggtt aaacttgcgt cgatcaatac cagaagattc gatcagcgca tccagttcac
     7681 ccactgcgtc gcgccactgc tgttttaccg tatcaatacg cgccacaatt tgcgcgtggc
     7741 gggaagccag cgtttcatca tcgggcggcg gtgctttgat aaccggtgct tcgccttgca
     7801 gataacgatt aatatcgcgc agcaacgcct gcggcccttt ccaggtttca aagaccacca
     7861 gggcaatttc acgcggcagc gggtagcagt ggcgacgcca gaaatcggcg caggcctggt
     7921 agcgtagcag agactcatct tcaatcagct gctgctcaaa cagcataccg gattcaaagg
     7981 cattcaggtt gagcatgcgc tggcaaaaac cgtgaatagt aaagactgcc gcttcatcca
     8041 tctgccgttc ggctaacaac aaccactgcg cggcttgcgc tttatcgtcg atctcttcca
     8101 gcaggcgttc gtacagtgga ttgtcggtgg tttcacgcag acaggcgatg cgcaactcgt
     8161 ggatattgct acggatacga ccgcgcaatt ctgccgtggc agcctcggta aaggtgacca
     8221 ccagcagttc ttcaacggtc agcgggcggg gaaaggcggc ggaaccgcct agtccaagta
     8281 acaggcgcaa atagagcgcc gcaatcgtaa aggttttgcc tgtgccggca gaggcttcaa
     8341 tcaggcgctc gccctgtaag ggcaagcgca aaggatctag tgtctcggcg acatcactca
     8401 ttcttttcac tcatcagggg cattgtttgc tgcaacgcgc tgacgttctc ccacactttc
     8461 cagccttcag gatgcacata ttcggctttc ccgttctggc tgccggaaat ctgcgacagg
     8521 atagccatgc cttgcggctc gaccaccgcc tgatggaaga aatcagcaag tttttgcggc
     8581 gtcagcagtt ttatctgggc cacgatttta tcacgcgaat cgaagcgcat attgccgcga
     8641 tcgaaatctt tacttaactt cgatgcttct tcgccgagcg tttgcggtgc ctgcagcatc
     8701 tgggtaatta ccgcctgctg gatttgcgca aactcatctg gcttcatcgc tcgcaatttt
     8761 gcctctgcgg ttgggaaaaa cgccttgtaa cgctcccaca agaatgaagg ctgtttatca
     8821 ttgctttgca aaaggaagcc catgccccac tgacgcccca cgctcattgg aaacgcaaac
     8881 acggcatagc ccaattgttc ttcggtacgc aactgattgt agaaccacgg ctgtacgatc
     8941 tgccccaaca gagagctata ggctgagctg gtgtattcat cgtagccagt cggtacaaat
     9001 accgctgcca gtgcggagtc ggtgctgtta ccggcttttt caaagatgac ggattgtttt
     9061 ttatcgacca ctacatcttt gtttcgacac cactctgaac catcagcgcc caactgtttt
     9121 tgcacatcgc gtgccagcgt tgttgcctgg gcctcggtca tgttgccgat aaccataaac
     9181 tctggtcgag cccctgattt taaggcgtcg cgataggcca gcacctcttt caacgtaatg
     9241 gagggcaaaa ttttacgccg ttcatctcgc gagaagtacg gcacttgcga gagcatctgc
     9301 gcgggcataa tcgcctgctc aaacgcttta cccttttctg cggaatccat catctggtta
     9361 taccaggact tcgcctgctc aagctgatct tccgtagcgg tatagctaaa gtacccctcg
     9421 agcaatgcct ggaacagctg cggcagacgc tgggtgtaac cattagcatt aaccataagg
     9481 ccgttgttag cgttggtgga aaaacttatg ccaccaaccg acgcctggtt gcttaactga
     9541 tcaagcgcca gccctgcgag ataatcattg agcgcaaaca tcacctgatt gcgggcgctg
     9601 tccatggctt tcggattacg caaaatcagg ctgacatcag ctttgggttc gctggaaaaa
     9661 taacggcttg gcgcatacac cacgcgcaga ttcgactcat caacaatcag ctctggatgg
     9721 tcatatttct tctctgactt aatcagcgag aaatcgtcag gaatataggg gttaagctcc
     9781 ggtaaggaga gcgcaatatt ggcagctttt tgctgccagt cagcgaaagt ttgttcgctg
     9841 attttatcga cctgatacgg cgcatcgaca aaataagccg ttttattgtg cggctctttc
     9901 gggctgatat accagatacg cgcattctgc ggcgtcatca tcgccagacg ttcttttact
     9961 gctttagcat cgtaccgatc ggcaatattg actgcatcca gcgtatgctc aaccggaacg
    10021 cgaatcatgg tatctgccag ccattcgacg taatccatat cacgggtgat cgacggataa
    10081 cggaagtcga tatccagcac attcgccagt tcatcgaagt attgtttatc aatgcctttt
    10141 tcacgtaaca gattgagata gctaaaaatt gccgccacaa cctgatcgcg attagccagg
    10201 cctttatcgg ttaaagacgc agagatcgct aatacgccgc tgttgccgtt gacgatagga
    10261 tcggagttgg cgctaatgcc ctcaactaat ccctgctttt gcagccagtc ggaaagtgta
    10321 cctgggctgc gattaccaat cagataggta atcaattcat ccgttttact acggaacttc
    10381 gctgagttgt tatcgatgcg aaactcaacg cgcagaactt tacgcggcag tgccgggacg
    10441 taatgaatga taatgccctt ttgcgcgtcg gtgactaccg gcacggtgat ttccggtttt
    10501 ttgctctctt tgttcggcac gcgaccaaag gtgtccgccg ccatttttgc caactccggc
    10561 agcggtttat tgctgtaaat aaccgccttc atcagattgg cggaatagta cttctcgtgg
    10621 aaatctttca gcgcctgctg caccggatta ccaggtttat cgcttaaagt ttcgaggtta
    10681 ccaccagaaa actttgaacc ggggtgtgcc gggttaatgg tttctgcgct gacctgtgcc
    10741 atgcgcatcc cgtcacgcgt acgcgccatg gttaattcag cgttcaccgc attacgctca
    10801 cgttcggcat atttcttgtc gagcaaaggt tcagcaatag catcggccag gcggtctacc
    10861 gcaccaggca aggcgtcgtt ctcaacttcc agatagaaag ccgtgcgata cggcgcagtg
    10921 ctggcattgt gactaccgcc gtgcattttg agatattcgg ccagactgtc agcctgcggg
    10981 tactttttcg accccatcag actcatatgt tcaaggtaat gtgccagccc ctggtacgcc
    11041 tcgggatctt ccagcgaccc aacgggcacc accagcgccg agagcgattt aactgcctgc
    11101 ggatcagaaa ccagcaagac caccataccg ttatccagac gtatagcctg atactggcgg
    11161 ttatctttat cacttttacg gatggtttcc tgaatcggct gccatcccgt ttctgcctga
    11221 cttaagggtg cccaaagggc aacaaacaac aataatgctt tgaaccaggt gctgcggggc
    11281 attcacggac ctcataatca actttatttt ctggccagat tcaacaggtt aacgcaaatc
    11341 atctgccaga atttaatctt gtgctgcacg agtcagccta tgtctatata tccatcagtc
    11401 cgtgactggt gcgcatcata atgtaagcga atgaattgcg caatttttat acagcactca
    11461 tgactgatta aagcgaaaca gcggtaacag gaaacgttgc gactgttcaa cgatggcctc
    11521 cattgtctct ggtgttaatt gccgccagag ccgttgatac cagatatcat caccttcgcc
    11581 acgcaccatc atgttgcctt cgtaagcctg aaggaatttc gtacgggctt tttgcaacgt
    11641 ggaatcgtca tccagcatgg catcgttttg cgcgtcataa caggttttta gccacgcgcc
    11701 gccactttca ggtaacacca gcaatggcgc ggacattcct tcacgatacc cctcaatcag
    11761 ttgtgagagg taatgcaaag cctgttcggc tgcaagcggc ggaaaacgcc actcgccgtc
    11821 tttgcgtaga aaaaggcgac tttcaccatt accaccgctg gcacagtaga caaggtgttc
    11881 cagccaaagt tgcattccct gggccacact taataaagag ggacgccagc gcaacaggcc
    11941 atccggctgc acctgcggca accagccagt tatctgcaca ccgttgcagg cgagatcaat
    12001 ttccatactc tgccccggct ggcgacaggc aatgactctg tcggcaagct gctgcatctc
    12061 ctggcactgt gtttcccaga aaatttcacc aaaagcgcca tacggtaaat cccctgccgc
    12121 ccggaagcgg cggaacaagc gttcggcatc atcctgctca accagtgcat tcaataactg
    12181 ctgattgatt tgataacggc taagcccttc cagaataaat ggctcggtgt cggggatttc
    12241 gctgtcttcg gtacggaagt tcacctgcaa acgcatctgg aaaaatgccc gcaccggatg
    12301 tgcccagaat cgttgtagcg tttccagcgg cacggtttcc ggtaaggtaa acggcagcgg
    12361 ctgaacaaat tcagaatgtg ctttaccagc ctggctggcc gcaggtagcc attcacgagc
    12421 ataactttgt cgttcgcctg gctggtagtt ttgcggatca aacggcattc gggtatgaag
    12481 gcaagtaaga tgcgatttta cccttgcctc gctttcatca cagttgagcg cctcatcgcc
    12541 cggtagataa tgactttgtc cgatgtagtc gatcagttcc tgcaccagta ccgacgggaa
    12601 acgttcactg ttatcctgaa tggaacgccc gatatagctg atatagagtt tttgctgcgc
    12661 ggaaattaac gcttccagga acagatagcg gtcgtcatcg cgacggctac ggtcgccacg
    12721 cttcggtttc tggctcatca ggtcaaagcc caatggcgca agctggcgtg gataaacgcc
    12781 gtcgttcatt cccagcaggc aaaccacttt gaatgggatg gaacgcattg gcatcagagt
    12841 acaaatgtta accggtccgg cgagaaaacg ctgactgata cgttcctgat ccaggcgctg
    12901 tgccagttca tcacgcaata gtgacagcgg caccgcgtcg ccatactgcg cacctaaacc
    12961 ttcggcgata atcgcctgcc attgttgttc gatcagcgtc atcgccgctt cggtttccgc
    13021 atccggcagg aagaaggcgt tgagcatatc gcgacaaacc ggcaaccact cttccagcgg
    13081 acgctcctgc gccagcccgc gacgccagat gtttagctgc attagcagtg aagccagatg
    13141 ccccaccagt tctgcaatta agccgctcga ttcatcataa ggtagaaccg attgccactc
    13201 gccctgcgcg ctctccatcg cgtagcccag caacatacgc gtcaggccaa accgccaggt
    13261 gtgttgaccg gtggcgggga gttccagctc gcgaacgtta tcgtcatcta tcccccagcg
    13321 aatgccggat tcgttgaccc actggcgtaa ataacgcagc ccttcttcgg tgatgtcaaa
    13381 ccgcgccgcc agcaccggca catccagcag cgccagcaca tcctctgaca caaagcgact
    13441 gtcaggcagt gataacaggc tgataaacgc ttccagtacc ggatgtgact gccgcgcccg
    13501 acggtcggaa atggcgtaag gcaggtaacg atccgcaggt gcgctaccaa acacagcctg
    13561 aataaacgga ctgtagctgt cgatgtcagc caccatcacg atgatgtcgc gcggagtaag
    13621 tgtcgggtct tcctccagca tcgccagcaa gcgatcgtgt aaaacttcaa cttcacgctg
    13681 cgggctatgg caaacgtgga aggtgatact gctatccagt ggatcaagcg ggcgtttgtt
    13741 atcgctacgg gaaaactctt cgatgttcac acccgcaacg gcgcggtttt ccagttccag
    13801 aatgtcagac tgaatgttat gcagcaggtt gtctggcgtc acatcgacaa aagcgtccag
    13861 ctcctggctg ctctccaggt cagaaaggag ataaatgtag tcgcgcccaa gcttccccca
    13921 tgaggccagc agcgggttgc cgacatcctg ttcaccatcg ctgttaaaga gctgtccggc
    13981 attttcgcta tcgcgaaata gcggcaattc gcgatcttca aaactgtgtc ggcgctggcg
    14041 agtcagtagt ttcgccagat aagcaggatc tttaatgtcg ccccagtaat aacggcaggg
    14101 gttggtaaac aggagatgga tttcaatatg tttacccagc gcctgtagcg cctggagata
    14161 aacaggcggt aacgcggaaa taccgcatat aaagacgcgc gaaggtagcc ccggcgggca
    14221 ggtcgtcgcg gactccagcg tttcgataaa gcgctgatag agattggcgc ggtgccagcg
    14281 cggttgcccg agttgatggg tatattccac caacgccttc cacaacggcg cttgccaggc
    14341 ctgtgcttct cccagccctt caaccaaatg tcctgtttcc cactgtgcca gccagtccgg
    14401 acgatagacc agatactggt caaacaggtc cgccgctttt gaagaaagct ggaacagttt
    14461 tcgcttgtcg ctatcgtcag tcagataatg ccgcaacagg gtaaagtctt cgcgctccaa
    14521 caattgcggc agcagagtca tcagtttcca gctcatgctc tgtttgttaa aggcgctctc
    14581 tttggggatt tccggtaaca cccggacgaa catatcccag ataaagctcg ctggcagcgg
    14641 aaaatcaatg tttgccgcaa taccaaactt ttgcgacagg gtcatttgca gccactgcgc
    14701 cataccggta ctttgcacca gaatcatctc tggttcgaaa ggatcgtcca gccgttcgcg
    14761 ttcgacaata aactccatca acgcttccag cacgtccaga cgattggaat ggtagaccct
    14821 taacatagcg gctcctgact actgacgatt cgggcaatgc aggcgggtca tctcgccttc
    14881 tctgcccccg ggtgaaacta gcgtaacgct gatgctgaca catcccgcct gcgatgtctg
    14941 cattcggttg acctgccagt tggcaggtgg cgaaatcgcg cgcagttgcg tttgctgcca
    15001 accatgccgc cagagctgtt ggtactggtt tctgctggcg agactgttca ttaatgttcg
    15061 ctggataccc gataacgcag tgacaatcat caccatcagc accatcgcaa ccattacctc
    15121 cggcaggcta aagccttgtt gattcttcag ggaagctgac ataacgccct ctctttcagc
    15181 ggacaaaaat cgctccagcc gcgtggcgaa aaaacaatgt ttccatcaat gacttcgcct
    15241 gttcgccaca acgaaacgcc ttcataaccg gcaatcaata aggcttcatt atctgccagt
    15301 aaacgcaaac aaacctgggc atcggttcca gcgtactgcg agcactgaac tgctggctgc
    15361 gtctgccagg agtgcatttt tccccacgcc agcgccgact gaacgatggc ctggcggcgc
    15421 aatgactgac tttccatgct cacgcgagag gcaaaactgc gatcctgctg actcattcct
    15481 tgcaatagca agctacccaa aaccagcagc atcaggacca gagccagtga cgaaacacct
    15541 ttttcgcggt tcacaggttg aatcctgtca cgctatagct ggcatccacc acggtttgcg
    15601 gttcagactt actggcagca cgcatattaa ccgtcaacac cggcgagaag ccgctgacat
    15661 cctgacgtac gacctgaaaa gtgtcgataa tgatggcatc cggattagtg actttatccc
    15721 agcccttacc ttcacaggat gtcgcaccgc gtagcgtttc cagcacatgc tccttcagac
    15781 gaaatccaat ctggtcagac tcttttaccg gttcgcgatc ccagataccg ttactgttcg
    15841 catcccactg cacaatgata cagtcaccct gtccgacaat ttccagccct tcgccggtac
    15901 agctgccatg acaataaccc gccctctgga gatgcttcgc gacggtaaat acccgcagcc
    15961 agatttcatc ttccagcgcc agcttacggg tgctcgttaa actttcacgc tgtaacgcag
    16021 gcagaaagcg tgcagccccc agcaacaata cgctactgat cgccatagca atcaacactt
    16081 ccagcagaga aaaaccttgc tcttttacag gcatccttct gtttctcctt gctgacaaag
    16141 ccggagtctt ccccacggcg aaaccaccag ccaccactcg cccgttgagt ttttgaagcg
    16201 aatatgcccg gcccatgcgg tattgcgcag gccaaagaaa gcaagcgaag gtgtcaggtc
    16261 gctcatttcg acttcgggcc agcgtggcac aaagaccaat ggtgaactgc catgacaggt
    16321 attggcccca gcagcggaac tcacaaggca ccataacgtc ccctccctga taacgctgat
    16381 actgtggtcg cggttatgcc agttggcatc ttcacgtaaa tagagcaaat agtcccgcgc
    16441 ctggctggcg gtttgccata gccgttgcga ctgctgccag tattgccagc catagagtcc
    16501 acttgcgctt agcatgacca aaatcagcat cgcgaccagc gtttcaatca gcgtataacc
    16561 acgttgtgtt ttcatgccgg cagtatggag cgaggagaaa aaaagacgag ggccagtttc
    16621 tatttcttcg gcgcatcttc cggactattt acgccgttgc aggacgttgc aaaatttcgg
    16681 gaaggcgtct cgaagaattt aacggagggt aaaaaaaacc gacgcacact ggcgtcggct
    16741 ctggcaggat gtttcgtaat tagatagcca ccggcgcttt aatgcccgga tgtggatcgt
    16801 agccttcaat ctcaaagtct tcgaaacggt agtcgaagat ggattcgggt ttacgtttga
    16861 taatcaactt cggcagcgga cgcggttcgc ggcttaattg cagatgagtt tgatccatat
    16921 gattgctgta caggtgcgtg tcgccccccg tccagacaaa atcgcccact tccagatcac
    16981 actgctgcgc catcatatgc accagtaacg cgtagctggc aatgttaaac ggcaggccga
    17041 ggaaaacgtc acaggagcgc tgataaagct ggcaagagag tttgccgtct gccacataga
    17101 actggaagaa cgcatggcac ggtgccagcg ccattttatc cagttcgcct acgttccacg
    17161 ctgaaacaat aatgcggcgc gaatccgggt cgtttttcag ctggttcagt accgtagtga
    17221 tctggtcaat atgacgacca tccggcgttg gccaggcgcg ccactgttta ccatacactg
    17281 gcccgaggtc gccgttttca tcggcccatt cgtcccagat ggtgacattg ttttcgtgta
    17341 gataagcaat gttagtgtcg ccctgcagaa accacagcag ttcatggatg atggaacgca
    17401 ggtggcaacg tttagttgtc accagcggga atccatcctg caggttaaaa cgcatctgat
    17461 gaccaaaaat ggaaagcgtt ccggttccgg tacggtcgtt tttctgtgtg ccttcgtcga
    17521 gcactttttg catcagttct aaatactgtt tcatggttcc tcaggaaacg tgttgctgtg
    17581 ggctgcgacg atatgcccag accatcatga tcacacccgc gacaatcatc gggatggaaa
    17641 gaatttgtcc catgctgatg tactgcaccc aggcaccagt aaactgcgcg tcgggctggc
    17701 ggaaaaactc aacaatgatg cgaaacgcgc cgtaaccaat caggaacaaa cctgagacag
    17761 ctcccattgg gcgcggttta cgaatataca ggttgaggat aataaacagc actacacctt
    17821 ccagcaacag ctcgtaaagc tgtgatgggt ggcgcggcag cacaccgtaa gtatcgaaaa
    17881 tggattgcca ctgcgggttg gtttgcagca gcaaaatatc ttctgtacgg gagccaggga
    17941 acagcatggc aaacgggaag ttcgggtcaa cgcggcccca caattcaccg ttaataaagt
    18001 tgcccagacg cccggcacca agaccaaacg gaatgagtgg tgcgataaaa tcagagacct
    18061 ggaagaagga acgtttagta cggcgggcga agataatcat caccacgata acgccaatca
    18121 ggccgccgtg gaaagacatg ccgccgtccc agacacggaa cagatacagc ggatcggcca
    18181 taaactgcgg gaaattgtag aacagaacat aaccaatacg tccaccgagg aagacgccga
    18241 ggaagcccgc atagagtaag ttttcaactt catttttggt ccagccactg cccggacgat
    18301 tcgcccgtcg tgttgccagc cacattgcaa aaatgaaacc caccagatac atcaggccgt
    18361 accagtgaag cgccacgggt cctattgaga aaatgaccgg atcaaactcc ggaaaatgca
    18421 gatagctact ggtcatctgt caccacaagt tcttgttatt tcgctgaaag agaacagcga
    18481 ttgaaatgcg cgccgcaggt ttcaggcgct ccaaaggtgc gaataatagc acaaggggac
    18541 ctggctggtt gccggatacc gttaaaagat atgtatatga tccgcgctat aaccctccgc
    18601 gaatcagccc gcccatgccg cgacgctcca taaaggctgc aacctgatgg cgaacttcgg
    18661 tcgccagttg cgcttccaga ctacgctgcg caagattttc tgcgtcggca taatcaatgc
    18721 gccgcagcag gtattttacc cgcgctacag aacgtccgtt catagacaga tggcgatacc
    18781 caagcccaat gaggattgcc acgcacatgg gatcgcccgc catttcaccg cacaaacgga
    18841 gatcgattcc atgtatttcc gcttcccggg cgatcatcgc cagagctcgt aacattgcag
    18901 gatgaagact gtcataaatg ttcgccaccc gggtattgtt gcgatcaacg gccagaatgt
    18961 attgagtcag atcgttggtg ccaacagaga tgaaatcgac ccgctttgcc agatgcggca
    19021 gcataaatac cattgacggc acttccagca tgatgccgat acgtggtttg ggaatttcgt
    19081 aaccgatcat ctcctcgact tcacgtccgg cacgttcaat caggcggcgt gcttcatcaa
    19141 cttcatcgag gcttgtgacc atcggcaaca gaatattcag gttgcccgta gcggcattag
    19201 cacgcagcat cgcccgcacc tggatcaaga agatctccgg ctgatcgagc gtaatgcgaa
    19261 tcccacgcca acccaggcat ggattctctt cgctgatcgg catgtaaggc agctgcttat
    19321 ctgctccgac atccagcgta cgcaaggtga cgggtttatc attgaacatt tgcagcatcc
    19381 cctgatactg cgccacctgt tcttcttccg acgggaaacc actttgcagc atgaatggga
    19441 tttcagtgcg ataaagaccg atgccatcaa tacggctgcc cagtttttct tcatgttccg
    19501 ggcttaaacc agcattgagc atgactttta tacgctcacc gctttttaac tgcgcgggta
    19561 aattgacgtc atcttccgcc agacggctaa gctcaatctc ttcactaatt agccgctgat
    19621 attcttgcag cagtaccggc tccggatcga ccagcaattc accgcgataa ccatcgacga
    19681 tcagcgtccg acgatgcagc accgaaggct gaatatccgc gcccatcacg gtagggatcc
    19741 ccagcgcacg taccatgatt gcagcatggg agttggctgc gccatcgcgc acgacaacac
    19801 ccactaagcg atcctggggc agctcagcaa gagttgtcgc tgacagttca tctgccacca
    19861 gaatgaaacg ttccggccag gcgttcggcc cttgattagc gtcatcaaga tgaaacagca
    19921 atcgctgacc cagcgcacgt aaatcgccag cccgctcttt gagatagtta tcgcttagcg
    19981 cggcaaactg ttcggcaaat ttttcaatga ccgtttttac cgcccactct gccaccgagc
    20041 ctttatcaac ctcggcaaac aagtcgcgac gcagccgggt gtccgaaagc aggtgcgagt
    20101 aaagatcgaa aatagccgcc gtttcttttt gtgctccggc ggcaaagcgt ttgctgtagc
    20161 ggcgaaactc gtttgccgct tcttccagcg ccccggtcag tcgttcgcgt tccagagccg
    20221 gatccagcgt tgatgcctga tacacctgtt ccattaaagg taacgtggca tcctgccagc
    20281 cttcggcaat cgccacacca ggtgctgccg gtaatgcgcg gattcgcgtc tggcgatatt
    20341 gcccaaacag ggcagtcaac tgcgactgag aaagaatagc tgccatctgg gtggcaagcg
    20401 tcaccaggaa ggattcttca ctttcgtcat actggcgcaa ctctcgttgc tgtaccacca
    20461 gtacaccaag caactggcga cgttgaataa ttggtacgcc taaaaacgcg cggaaacgtt
    20521 cttcttttac ggaggggatg tatttgaagc tggggtgctt ttgcgcatct gcaaggttta
    20581 tcggttccgc cagcctgcca accaggccga cgatcccttc atcaaacgcg agcgttacag
    20641 tgcgaccgcg tggttttttc agcccccggg tcgccatcag gtagtaacaa cgtcgatcat
    20701 gatcggccag gtagaccgaa cagacctcgg tatccatcgc aagacagatg tcggtaacca
    20761 gaatatttaa cgcctcattc aggcgtggtg cgctggctac cttttcgact atttcgcgca
//
LOCUS       DAMZET010000072        20442 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.072, whole genome shotgun sequence.
ACCESSION   DAMZET010000072 DAMZET010000000
VERSION     DAMZET010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.072"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            156..1421
                     /locus_tag="POB34_002652"
     CDS             156..1421
                     /locus_tag="POB34_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDI5919571.1"
                     /translation="MALTDIKVRAAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARVRRDEARNLLANGVDPGDKKKNDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEV
                     AARLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASCNRQHRPALELKRIPELLTKIDS
                     YTGRPLTRWAIELTLLIFIRSSELRFARWSEIDFEASIWTIPPEREPIPGVKHSHRGS
                     KMRTTHLVPLSTQALAILKQIKQFYGAHDLIFIGDHDSHKPMSENTVNSALRVMGYDT
                     KVEVCGHGFRTMACSSLVESGLWSRDAVERQMSHMERNSVRAAYIHKAEHLEERRLML
                     QWWADFLDVNRERFISPFEYAKINNPLKQ"
     gene            complement(1738..2919)
                     /locus_tag="POB34_002653"
                     /pseudo
     CDS             complement(1738..2919)
                     /locus_tag="POB34_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            3506..3735
                     /locus_tag="POB34_002654"
                     /pseudo
     CDS             3506..3735
                     /locus_tag="POB34_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            4078..4221
                     /locus_tag="POB34_002655"
     CDS             4078..4221
                     /locus_tag="POB34_002655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919572.1"
                     /translation="MSLDPLACGHDNVKQSKIVALTVSLGDKKAQYSRWQPDNLTVDA
                     ETK"
     gene            complement(4249..5595)
                     /locus_tag="POB34_002656"
     CDS             complement(4249..5595)
                     /locus_tag="POB34_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HDI5919573.1"
                     /translation="MFPDFFMHIGQALDLVSRYNSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRHVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY
                     YSLGLLNAWSLAGEHRHWMIPLRKGAQYEEIRKLGKGDHLVKLKTSPQARKKWPGLGN
                     EVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMGDLYSHRWEIELGYREIKQTMQRSR
                     LTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLM
                     TLQGASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQSVA"
     gene            6204..7421
                     /locus_tag="POB34_002657"
     CDS             6204..7421
                     /locus_tag="POB34_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5919574.1"
                     /translation="MATAWYKQVNPPQRKALFSAWLGYVFDGFDFMMIFYILHIIKAD
                     LGITDIQATLIGTVAFIARPIGGGFFGAMADKYGRKPMMMWAIIIYSVGTGLSGIATN
                     LYMLAVCRFIVGLGMSGEYACASTYAVESWPKNLQSKASAFLVSGFSVGNIIAAQIIP
                     QFAEVYGWRNSFFIGLLPVLLVLWIRKSAPESQEWIEDKYKDKSTFLSVFRKPHLSIS
                     MIVFLVCFCLFGANWPINGLLPSYLADNGVNTVVISTLMTIAGLGTLTGTIFFGFVGD
                     KIGVKKAFVVGLITSFVFLCPLFFISVKNSSLIGLCLFGLMFTNLGIAGLVPKFIYDY
                     FPTKLRGLGTGLIYNLGATGGMAAPVLATYISGYYGLGVSLFIVTVAFSALLILLVGF
                     DIPGKIYKLSVAK"
     gene            7433..8551
                     /locus_tag="POB34_002658"
     CDS             7433..8551
                     /locus_tag="POB34_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDI5919575.1"
                     /translation="MINYGVVGVGYFGAELARFMNMHDNAKITCVYDPENGENIAREL
                     QCINMSSLDALVSSKLVDCVIVATPNYLHKEPVIKAAKNKKHVFCEKPIALSYEDCVD
                     MVKACKEAGVTFMAGHIMNFFNGVQYARKLIKEGVIGEILSCHTKRNGWENKQERLSW
                     KKMKEQSGGHLYHHIHELDCVQHLLGEIPETVTMIGGNLAHSGPGFGNEDDMLFMTLE
                     FPSGKLATLEWGSAFNWPEHYVIINGTKGSIKIDMQETAGSLRIGGQTKHFLVHETQE
                     EDDDRRKGNMTSEMDGAIAYGHPGKKTPLWLASLIRKETLFLHNILCGAKPEEDYIDL
                     LNGEAAMSAIATADAATLSRSQDRKVKISEIIKHTSVM"
     gene            8594..8719
                     /locus_tag="POB34_002659"
     CDS             8594..8719
                     /locus_tag="POB34_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919576.1"
                     /translation="MIKNFIFDNLIILAVPFMIKTSLKTNLIFFFLCVFVPHMAS"
     gene            complement(8772..9029)
                     /locus_tag="POB34_002660"
     CDS             complement(8772..9029)
                     /locus_tag="POB34_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919577.1"
                     /translation="MVSSSASNVVNCETKQRTQFECIYFSQYWAKGDFIAKRAPIGQW
                     EPYSEESLLGIIVTSVCRIKVAMLKPEPPRDPHIPLMGDFN"
     gene            9019..9255
                     /locus_tag="POB34_002661"
                     /pseudo
     CDS             9019..9255
                     /locus_tag="POB34_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939276.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            9343..10509
                     /locus_tag="POB34_002662"
                     /pseudo
     CDS             9343..10509
                     /locus_tag="POB34_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            10592..10849
                     /locus_tag="POB34_002663"
     CDS             10592..10849
                     /locus_tag="POB34_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919578.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(11406..12173)
                     /gene="fecE"
                     /locus_tag="POB34_002664"
     CDS             complement(11406..12173)
                     /gene="fecE"
                     /locus_tag="POB34_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HDI5919579.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(12174..13130)
                     /gene="fecD"
                     /locus_tag="POB34_002665"
     CDS             complement(12174..13130)
                     /gene="fecD"
                     /locus_tag="POB34_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HDI5919580.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(13127..14125)
                     /gene="fecC"
                     /locus_tag="POB34_002666"
     CDS             complement(13127..14125)
                     /gene="fecC"
                     /locus_tag="POB34_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HDI5919581.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(14122..15024)
                     /gene="fecB"
                     /locus_tag="POB34_002667"
     CDS             complement(14122..15024)
                     /gene="fecB"
                     /locus_tag="POB34_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HDI5919582.1"
                     /translation="MLAFIRFVFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(15069..17393)
                     /gene="fecA"
                     /locus_tag="POB34_002668"
     CDS             complement(15069..17393)
                     /gene="fecA"
                     /locus_tag="POB34_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HDI5919583.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLTSRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(17480..18433)
                     /gene="fecR"
                     /locus_tag="POB34_002669"
     CDS             complement(17480..18433)
                     /gene="fecR"
                     /locus_tag="POB34_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HDI5919584.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(18430..18951)
                     /gene="fecI"
                     /locus_tag="POB34_002670"
     CDS             complement(18430..18951)
                     /gene="fecI"
                     /locus_tag="POB34_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HDI5919585.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            19243..19940
                     /locus_tag="POB34_002671"
                     /pseudo
     CDS             19243..19940
                     /locus_tag="POB34_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(19960..20025)
                     /locus_tag="POB34_002672"
                     /pseudo
     CDS             complement(19960..20025)
                     /locus_tag="POB34_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
     gene            20082..20264
                     /locus_tag="POB34_002673"
                     /pseudo
     CDS             20082..20264
                     /locus_tag="POB34_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            20258..20362
                     /locus_tag="POB34_002674"
                     /pseudo
     CDS             20258..20362
                     /locus_tag="POB34_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         5056 a         4981 c         5311 g         5094 t
ORIGIN      
        1 ttttttatgc ctgaaatcta gggtttatct atctttcccg ctatattaac tctctcaagg
       61 tcaaccgata tcaacgtaca tctaccaaca tatgttggta cagatgatgg tatttccggt
      121 tcgataatgc ttgtaccaac agggagggaa tacgcatggc attaacagat atcaaagtca
      181 gagcagccaa gccaacggat aagcagtata agctgactga tggtggcggt atgcatctgc
      241 ttgtccatcc aaatggttct aagtactggc gtttgcagta ccgttatgag ggaaagcaaa
      301 aaatgctggc acttggggtt tatcctgaaa tcacactagc ggatgccaga gtacgtcgtg
      361 acgaggcgcg taacctgctt gcgaatggcg tcgatccggg agacaaaaag aaaaatgata
      421 aggttgaaca gagtaaagca cgaaccttta aagaagtcgc gattgagtgg catggcacca
      481 ataaaaagtg gtctgaagat cacgcccatc gtgtgctaaa aagtcttgaa gataatcttt
      541 ttgcagcgct tggtgaacgt aatatcgctg agttaaaaac tcgagattta ttagcaccta
      601 ttaaggccgt agaaatgtct ggacgtcttg aagtggccgc tcgtcttcag cagcgcacta
      661 cagccatcat gcgctatgca gtgcaaagtg ggttaattga ttataacccg gcacaagaga
      721 tggctggggc ggttgcttcc tgtaatcgac aacatcgtcc cgcgcttgaa ttaaagcgca
      781 tccctgagtt gcttacaaaa atagatagct atactggtag gccgctaacc cgatgggcga
      841 tagaactcac tttgctgatc tttattcggt ccagtgagct gcgttttgct cgttggtcag
      901 agatcgattt cgaagcgtct atatggacta tcccaccgga gcgggagcct attcctggag
      961 tgaaacattc ccatagaggc tcaaaaatgc gtacaacgca tctagtgcct ctttcaacgc
     1021 aagctcttgc aattttaaag cagataaaac agttttatgg ggcccatgac ttgatattta
     1081 ttggtgatca cgattcgcac aaacccatga gtgagaatac ggtaaatagt gcgttacggg
     1141 tcatggggta tgatacaaaa gtagaggttt gtggtcatgg ctttcgaaca atggcctgta
     1201 gttcattggt cgaatcaggt ttgtggtctc gtgatgctgt tgaacgtcag atgagccaca
     1261 tggagcgaaa ttcagtgagg gccgcgtata tccataaagc agagcatctg gaagaacggc
     1321 gattgatgct acagtggtgg gccgattttc tggatgtaaa cagagaaaga tttatcagtc
     1381 catttgaata tgcaaagatt aataatccat taaaacagta atcatcccgg gcaaatgccc
     1441 gggaattatt ctaggattat tttctttgtt aaaaaagaca aacggtatta actgatgtat
     1501 ttactattta ccgctccctg caggggggat tatcagaatc cgcggttatt cattatttat
     1561 atactttcaa taaaaaagta acctcataaa aaatcctgta ctcacagggt gatgcgggat
     1621 aactgacgta aggagtgctg cagggatttg atgatccctt gtgctgatat gaatacggat
     1681 attttataca gaatactttt cagtacaagc gactgtatta cagtctgcca tatatgatta
     1741 cctgtgatca ggaagaataa gtggctgagg tttcaaactg tctgccggta tattcctctc
     1801 tcccttaaaa accataacag tttaattatc ttcgtctgtc agcggattga atggcggtat
     1861 attttcagta caaataccgg tcatccactg aaaaatacct gcgaaatgac gggcactgat
     1921 ttttctactg acgggctgat gagacgttat gtcactggcg gtaataatca gtggcacact
     1981 gtagcctccc tgcacatgac cataatgatg aacaggattg gcactgtcgc tgaccgacag
     2041 cccaaggcca gaaaagtaaa gcatggcgaa atgacgtgag taccggcgaa ggataccatc
     2101 aagctggcca agaaagttat ccagtttact gatgctggcg aggtaacagg caatttttcg
     2161 gggatactgc tccaggtaat tattcggcca ggagttaagg ctgtcacacg gatttggatg
     2221 agaacccatc atgtgcagga aaattatctt cggagaggat gtatccgcca gcgcacgttc
     2281 tgtttcctgt aacaacagtt tgtcatctgt tttacgggaa gcgaagctgc ctttcttgag
     2341 gaaagtggca tgctccgcat cagaagcaat aacagagatg cgtgtgtcat gctcccccag
     2401 ttttccttga ttggatatcc accatgtgct gtatcctgct tttgctgcca gcgccaccac
     2461 attgttgccg gaatcagggt tctgctcata gtcataaatc agtgtccggc tcagggaaga
     2521 cactgtactg gaggctgtcg aggtataatc gtcaataaat aaaccgggtg ccgtattcag
     2581 ccacggtgtg gttggtacgg gatagccata cactgacata taatccctgc gcacactctc
     2641 accagtgaca ataacaatcg tatcatataa cggtgctccc ggctggattt tccagttgtc
     2701 agccccgtgc tgactcagtt gttcataacg ctgcatttca cgtaatgtgt cagttgtccc
     2761 cacaactgtt cctttaacca tccgcaatgg ccaacggttt acagagcata atacgaatag
     2821 ccgtagagcc agccagttac ggtggtcgcg atggtgtgtc cgccagaaaa tcaccacgaa
     2881 taccagaatc gcggcactga ccagaaaatg ataaacagga aacacggggc ggttcacttt
     2941 gctttctcct gtagtgtttt ctgtaggttc tgaaccggtt cgagcggggt tgtgaggctg
     3001 atggcaatcc ccgggctctt cctgcatgtt acactggtac tgcaagaggg ggccttcgct
     3061 cctctgacat tactcagtat tttcactact acaccccatc tgccatccat aacaaccgat
     3121 tcaccccctc gcgaggcgtt gttgcccgtc acccggacag acatcgttat ggacttctct
     3181 tgttgcacga tcgatcaatt gttatgcatg ctgacaccac aaccccggtg ggtatgaccg
     3241 atgcctttct cactctcttc ccggtcatcg tcggccttcc ccgttattgt ggcgtgtcag
     3301 caccctcgac ggcatttcga tggctgctca gtgttcactc acgttacggc ccgcataacc
     3361 cgttgacttc ctgaagaagt cgtttcaggg agagattcag cccttttgtt acctcctgga
     3421 ccgcttcttg tccttccagt cggagcgaga gttagccggg gcggactttc accgacggaa
     3481 agatcgtgca tttcaaggca cacacaacaa tatctgcgag cgtgctatcc gtccggtcgt
     3541 tatgggacga aaggcctggt tgttcgctgg ttcactgatg gccgggaacc gcgcggcaca
     3601 gataatgagc cttctgggaa ccgcagtctg gagccacatg cttggctgac ggacgtcctg
     3661 acgcgtctgc ctgagtggcc ggaggagagg ttggctgagt tactgcctct tgagggcttt
     3721 accttcttcg ggtaagtgat acctgccgtc aggtgtttgt gcaccgggcc ataacctgca
     3781 gttgggaatt gaacgcctga caacaggaaa tgagctagag cagtaaacac gcgggctgac
     3841 aatgggtcag aatatgagtc agcccggcgt tattcctggg gtcagtactt tagatccagc
     3901 ctccggtacc agactagcta gcccctacgc aaccaaataa atcaaccatc gtcagagtgc
     3961 aatcgttcat atacactgtc ggtgatgcgc tgaaaaatgc gttaatgttg agttcctcga
     4021 acccttacca attaagggac atagccagat acgcaactgg ctgatgcaag cagtctaatg
     4081 tctttagatc cgctggcctg cgggcatgac aatgtcaaac agtcaaagat tgtggcgttg
     4141 acggtcagtc ttggggacaa aaaagctcag tattctcgtt ggcaaccaga taatttaaca
     4201 gttgatgctg aaacgaaata ggcatcttgg ttctgtaatg ccagtcagtt aagcaactga
     4261 ctggctcttt ttcggggctg tggggtattt ccagggcctc tcctttacca ctctcgggaa
     4321 ggccctttcc cttcttgtcg gtaatttcac aagttgtccc atacttgcaa gatcgcgcat
     4381 cagctccggt atacgtcccg gtgaagcgcc ctgcaatgtc atcagcattc tcatcaccat
     4441 tccgcatgat tctgagaaac tcagttgatt cggccagtaa cctttcagat gttccgccat
     4501 tttaatcatc tgatatctca ccagattata agccagtaag acaccccaca gctcttgctc
     4561 cacaagctcc ggctttttac ttctcagcgt cagcctgctc cgttgcatcg tctgttttat
     4621 ctccctgtat cccagttcga tttcccagcg atgactgtac agatccccca tttctcctcc
     4681 ggggaagcgc atggcgtccg tcatcgacgt cagcagatgg cagacttttc ctttgcgcgt
     4741 cacggtcagc aggcgggcag tcacttcatt tcccagtccc ggccactttt ttcgtgcctg
     4801 cgggctggtt ttcagcttca ccagatgatc gcctttaccc agttttctga tctcttcata
     4861 ttgcgctccc tttctgagag gtatcatcca gtggcggtgt tctcccgcca ggctccaggc
     4921 atttaacagt cccagtgagt aataaccttt atccattaac gtcagagtgt tatcgccggt
     4981 ttgttctata agttgctcag caagctcatt ttcgctgttc ttcatcgtgc cgaaggctgc
     5041 agccgtcagc agatggctgg tcagttccat ctggcagacc attttgacct gcgggtagag
     5101 cgccgggttc ccggcatgtg tctggcgggg gaaggctgca tcgttctctg gtgtatccgg
     5161 tgtgcgccag aacacaccat cgatggccag cagggtcagg ccgcaccagt gcggatgcgg
     5221 cgtggcgtta tgccagagct gcgctgtttt cgtgaacacg tggcggacag cctcacttcc
     5281 caggcgctgg cgggcctgaa taacggcact gggggcaacg aaggggcgat tgcccggcag
     5341 catgatgtcc aggcgattca caatctggtg aagaggttct ttacgctcaa gcgccatgcc
     5401 aacaatacac cagaccatca tttcgagggg aagacggcgc ttgcgtagcg ttacagtacc
     5461 tgattcggca aggcaacgag agatgagttc ggggtcgagg taatccccca gagaagtcag
     5521 tgggttacgc agagaattgt aacgggatac cagatcaaga gcctgtccaa tgtgcataaa
     5581 aaaatccgga aacaagtgag cgtttccgga ttcttacaca gccactggat cggtcaactg
     5641 atccttaact gatcggcatt aatcttggtt ctggtgtttg taacaaacta tcagctacaa
     5701 aaatatgctc aatttgtgac atcagtaaca aaacgcgttt tgttatgtgg attgcttgtt
     5761 ttttgatctg gtataacagg tataaaggta tacagaaaag caaagaaata ctgcaaagga
     5821 aaacagctat aacgtaagct aaagtaataa cctctcagtc tttcctcatt tgactaaggg
     5881 agttttattc aacctgaacg gactacgaaa atgagcacaa tgaataagtc aatttttgaa
     5941 gcagggttga actgtgccag tggtggaata tctggcgaac attgtttttt gttggtgacc
     6001 caaaactgta ggtgggtatc agttttatct ttcatagagt gaaatatgtt aagaagaaat
     6061 ggaggaaaag attgtacgga ttaggtattg ataacaatca atagtactgg cgtatttgaa
     6121 gacaatataa ttatttctgg atattgttga ggctccctaa tatttacttt aagggctata
     6181 ttagaataac acaggaaaca aatatggcaa cagcatggta taaacaagtt aatccaccac
     6241 aacggaaagc tcttttttcc gcatggcttg gatatgtatt tgatggcttt gattttatga
     6301 tgatatttta cattcttcat attataaaag cagatcttgg cattacggat attcaggcta
     6361 ctttaatagg gacagtggcc ttcatagcca gacctattgg aggtggtttt tttggtgcca
     6421 tggctgataa atatggtcgt aagccaatga tgatgtgggc aattatcatt tactcagtcg
     6481 gaacaggcct tagcggtatt gctacaaact tatatatgct cgcagtttgc cgttttattg
     6541 ttggcttagg gatgtctggt gaatatgcat gtgcttcaac ttatgcggta gaaagttggc
     6601 ctaaaaatct tcaatctaaa gctagtgctt ttttggtaag tggtttttct gttggaaata
     6661 ttattgcggc acaaataatc cctcagtttg ctgaagtata tggatggaga aactcttttt
     6721 ttataggcct gttaccagtt ttactagttc tttggatcag aaaaagtgct ccagaaagtc
     6781 aggagtggat tgaagataaa tataaggata aatcaacttt tttgtctgtc ttcagaaaac
     6841 cacatctttc aatctctatg atcgttttcc tcgtctgttt ttgtctattt ggtgcaaact
     6901 ggccgataaa cggactactt ccttcctacc tggcagataa tggagttaat acagtggtta
     6961 tttcaactct gatgacaata gcaggtttag gaacactgac aggtacaata ttttttggtt
     7021 ttgttggtga taagattggt gtaaaaaaag cctttgtagt cggtctaata acttcatttg
     7081 ttttcctttg tcctcttttt tttatttctg tgaaaaactc ttctcttata ggattatgtc
     7141 tctttggatt aatgtttaca aatttaggta ttgcagggtt ggttccaaaa tttatatatg
     7201 attactttcc aacaaaatta agaggattag ggaccggtct tatttataac ttaggggcaa
     7261 ctggaggaat ggccgcacct gtattagcta catacatttc aggatattat ggcttaggtg
     7321 tttcattatt cattgttacg gttgcattct ctgccttatt aattttgtta gttggttttg
     7381 atattccagg taaaatttat aaactatccg tggctaaata attggaggct ttatgattaa
     7441 ttatggcgtt gttggtgttg gatactttgg cgctgaatta gctcgtttta tgaatatgca
     7501 tgataatgca aaaattacat gtgtatacga tcctgaaaat ggagaaaata ttgcccgtga
     7561 attgcagtgt atcaatatgt caagcttgga tgctttagtc tcaagtaaat tagtcgattg
     7621 cgtgatcgta gccaccccaa attatctgca taaagaacca gtaattaaag cagcaaagaa
     7681 taagaagcat gttttttgtg aaaaaccaat tgcattaagt tatgaagatt gtgtggatat
     7741 ggtcaaagcg tgtaaagaag ctggtgtgac ctttatggcc gggcatatta tgaatttttt
     7801 caatggggtt caatatgcac ggaagttaat taaagaaggt gttatcggcg aaatattatc
     7861 atgtcatact aagagaaatg gctgggaaaa caaacaagag agactttcct ggaaaaagat
     7921 gaaagaacaa tctggtggac atctatatca tcatatacat gagttagatt gtgttcagca
     7981 tttacttgga gaaataccag agacggttac tatgattggt ggaaatttgg cccattctgg
     8041 tccaggattt ggcaatgaag atgatatgtt atttatgacc ttggaattcc cgtcaggaaa
     8101 actagcaacc ttagagtggg ggagtgcatt taactggccg gaacattatg tcatcatcaa
     8161 tggaactaaa ggctctatta aaattgatat gcaagaaaca gcagggtcac ttaggattgg
     8221 cggtcagaca aagcattttt tggtccatga aacacaagaa gaagatgatg atcgtcggaa
     8281 aggcaatatg acctcagaaa tggatggcgc tatagcatat ggtcatccag gaaaaaaaac
     8341 accattatgg cttgccagtt taataagaaa ggagacgtta ttcctccata atatcctctg
     8401 tggtgcaaaa cctgaagaag attatattga ccttctcaat ggtgaggcgg ccatgtcggc
     8461 gattgctact gctgatgctg ccactctttc aagatcgcag gacaggaaag tgaaaatcag
     8521 tgagatcatt aaacatacat cagtaatgta aaaactatac gcctcttgat ttaattcagg
     8581 aggcctttta tgtatgatca agaattttat cttcgataat ctcataattt tagcagtgcc
     8641 atttatgata aaaacatccc ttaaaaccaa tctaatcttt ttcttcctct gtgtatttgt
     8701 tccccatatg gcgtcataat ttacgctacg taatacggga tagttacgat acgcagcgat
     8761 agcgctaagt tttagttaaa atcccccatc agcgggatat ggggatctct gggaggttca
     8821 ggttttagca tcgcgacttt aatacgacag acgctggtga cgatgatgcc aagtagtgac
     8881 tcttccgaat aaggttccca ctgaccaatt ggcgcgcgct tcgcaataaa atctcccttc
     8941 gcccagtatt gggaaaagta gatacattca aactgtgtac gctgtttcgt ctcacagttc
     9001 acgacattgc tggccgatga gctgaccatt gggcctatcc gggctgtccc gatggatatt
     9061 acgccgaagt atgtgggaat tgccagcgga ttgatgaacg ccggttccgc tgtcgccgac
     9121 attatttcac ctatcgcctt tggcattatt atcgacaaaa caggcaactg gagtttacca
     9181 ttctacggtt ccgtcgcatt gcttgttatt ggtatattcc tgacgttctt catgcgtcca
     9241 gataagtcat tgtaaaagtc gaatcagggc tgaagtggca cactgaattt ggccacctga
     9301 acagaggtga tatgctcacc tcagaacaac acaggtgctc caatgaaaaa aagaaatttc
     9361 agcgcagagt ttaaacgcga atccgctcaa ctggttgttg accagaaata cacggtggca
     9421 gatgccgcga aagctatgga tgttggcctt tccacaatga caagatgggt caaacaactg
     9481 cgtgatgagc gtcagggcaa aacaccaaaa gcctctccga taacaccaga acaaatcgaa
     9541 atacgtaagc tgaggaaaaa gctacaacgc attgaaatgg agaatgaaat attaaaaagg
     9601 ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga aactcagagc
     9661 gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca gctacagata
     9721 ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca gtcaggtact
     9781 tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca caatggcaac
     9841 ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag agctggggct
     9901 ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac atgttgctat
     9961 ccctaactac cttgaaaggc agttcgccgt gaccgagcca aatcaggtgt ggtgcggtga
    10021 tgtgacctat atctggacgg gtaagcgctg ggcgtacctc gccgttgttc tcgacctgtt
    10081 cgcaagaaaa ccagtgggct gggccatgtc gttctcgccg gacagcaggc ttaccatgaa
    10141 agcactggaa atggcatggg aaacccgtgg taagcccgtc ggggtgatgt tccaagcgat
    10201 caaggcagtc attatacgag caggcagttc cggcagttac tgtggcgata ccggatcagg
    10261 cagagtatga gtcggcgtgg aaactgctgg gataacagcc caatggagcg cttcttcagg
    10321 agtctgaaga acgaatgggt gccagcgacg ggctatgtaa gcttcagcga tgcagctcac
    10381 gcaataacgg actatatcgt tggatattac agcgcactaa gaccgcacga atataatggt
    10441 gggttaccac caaacgaatc agaaaaccga tactggaaaa actctaacgc ggaggccagt
    10501 tttagttgac cacaacagac tacctgaagg gagccgcggt cgcctggcag ttgcagtagc
    10561 aggagatcat ccagccgcag tacaggtcac gatgactctg gttaatgata ccggctttga
    10621 ccccgtattt tccggctcta tcgctgaatc atggcgtcag cagccgtgca caccatccta
    10681 ttgttgtgac tgggaggctg ccaccatgct tcgcgctttc cctctggcga aaaagggaga
    10741 aggacgggcc cgtctgcctt cactttatgc cagcttcggt aagctgggtg agacaccgac
    10801 tcatgaagat atcattgata acaatcgatc catcaactgg cctgtataac gtggctgccg
    10861 gtgattaaga aagctgcacc tacctaagta gtagcaaacg cacacttttt agaaaaatcg
    10921 atggtcagaa actggattag caattccgtt ccagggttgc ttttgattta cgttggcgtc
    10981 tgatcattga tttatcctca aaagcccaac ctcattggta atgaaccagc tccgtgaatg
    11041 tccgctctgg cacagagcga aattttttga tctcccccct gaaatctaaa cttagtcatg
    11101 tcacgttttt gggtttctaa aattttaact tcgcgttttt cgttgccgta agggttatac
    11161 agaaatgtcc gttaagcaga gttcaaaatt gattgccgtg atcacgactg gtttgaaagc
    11221 cgcgcccaag cctgtacagc tctggtttgc gttgattatg aacctgtcag cctaaagcaa
    11281 gcggatggac gatgagtatt ggtaatcttt cagagtccgg aaaagttcag ccccagtctg
    11341 aacaggcttg ctggcgccag tccagtttca ttcagtcgtg gtttggttct tacggcctgt
    11401 gcaatctacc tcattaggca catcggcctg ccagataccg gctcggggtg tatttccgct
    11461 tccacgctga atactgttct cagcaatcct ggggtcatca cctcttctgg tgtgccttgc
    11521 gccataacat gtccgtttgc cattaccacc agttgatcgc agtaccggct agcctgatta
    11581 aggtcgtgca gcacagcgac caccgttttc ccctgagtcc ggagttcgcc catcaaccgc
    11641 atcaggtcca cctggtgatt gatatcaaga taggtggttg gctcatcaag taatacaacg
    11701 ggcgtattct gggccaggac cattgccaga aatgcgcgct ggcgctgacc gccggaaagc
    11761 tcggttaacc gacgaacggc aagatgattg atccgggtct ggttcatggc gacattaact
    11821 cgtgcattgt cttcagcgga gagacgcccc cagagtgaca gccagggatt acgaccatac
    11881 gaaaccagct cctggactgt gatcccctct ggcgttaaat ggtgctgagg cagcagcgaa
    11941 agcctgcggg ccaactggcg cgatgagagc atatttatgg gattatcgcc gagaaatacg
    12001 gtgccagact gcggcattaa aagccgcgaa aaacagttta acagcgtcga tttcccgcaa
    12061 ccgttaggac cgatcagggc ggtgatcttc cccgttggca gtgagagtga aacgtcgtta
    12121 agtaccttgt ctgtcccgta actgaccgtc agattttcag ttcgtaaagt catttatcgc
    12181 attctcacaa gcaaccagac aaaccacggc gcaccgataa tggcggtcag cacgccaacc
    12241 gggagctcca gtgggggatg aataattctc gccagcagat cggcaaccac caacagcaac
    12301 gcacctgtca gggccgaaac aggcagcagt ctgcggtgac gtccaccggt gatgctacgc
    12361 atcatatgcg gcaccacgag accaataaag ctaatcgggc cgcaggcggc cacgccggta
    12421 gatgtcatgg cgacagctag taacaaagcc cagaatcggg tatggggcac cgacacaccg
    12481 agcgtggtgg cgcgcgcatc gccgagtgca aggaggtcga gatcgcggca aaaactcagg
    12541 ctcagcggca gaaataaaat catcagcggg atggcaatct tcacaaagct ccagtcacgg
    12601 ccccataagc tgccggtcag ccacagcagg gcgttgttca catcctgcgg gcgcgagagc
    12661 atcagataat ccgtcaggct ggcccagcat gcagaaagcg ccacgccggt gagcgccagc
    12721 ttcatcggct ggtgggtctt tgccagcatc ttcagcaata tcaaccccgc catgccgccc
    12781 gcaaaggcca gcagcggcag caccatcacg ggcagtgacg gcataagaag tagagccccc
    12841 acagaggcca ggctggcggc atggttaaca ccgagaatat ccggtgatgc cagagggttg
    12901 cgcacaatcc cctgtatcag cacgcccgcc acggcgaggg ctgcaccgac aaacagtgcc
    12961 agcagcaagc gcggcagtcg gtactccatc aatacataat aatgctcgcg tccggcctgc
    13021 cagtcggtca gcagcgcgcg ccacggcacg gggatcactc ccatatggag tgataacagc
    13081 gcacagcccg ccagggcaag ggtgatgaaa ataaccagcg caattttcat cctcgcctcc
    13141 tcacaagcca gacaaagcaa gggctgccaa tcagcgccag cactgcgcct gcgggcagat
    13201 ctccggggaa ggccagtgcg cgtgcgagta catctgccag cagcatcagc gtggccccca
    13261 gcagcatgct cactggcagt acgttgcgct gatcgaagcc tgcccagaag cgcgccagat
    13321 gtggcaccag cagaccgata aacgccaccg gacctgccac gctgacgcac gcaccaacca
    13381 gaagcagcac taacatattg atgaccaaac gtagcctcgt caggttcact cccagcgtat
    13441 gggcggtgct gtcgctgagg ttgagcaggt tcagttgatt cgccagcagc aacacgacag
    13501 ggactgcagt gaccaccacc ggcaagagct gccagacatc ctgccagcgg gcgtgggaca
    13561 ctccgcctgc cagccagtaa aagatgccgt aagcatgatc ttcggccagc agcagggtga
    13621 tgcgggtaag gcccatacaa aaggccgaca gcgcgatacc cgcgaggatc agtttgtttc
    13681 tgtcatgggt atgacgaaat ccgcctcctg cggtcatgac cagcagccag ctcacgccgc
    13741 ccccgcatgc cgcgatgaac gacagagaat agcctgcaat cggcgtcgga ctcagcgcgc
    13801 tggtaagcgc catagccagc gccgcgccgc tgttaatgcc gagcagtgaa ggagaggcca
    13861 ttgggttgtg ggtcagggtt tgcagcagcg tgcccgcgag cgccaggctt gcgccgatca
    13921 gaacggcgac caggcttcgt ggcaaacgaa ggttttgcac cagcgcttct ggtagcgttg
    13981 gcgtgtgtcc aggcagcagg gcgcgggttg catctgctcc ggaaacagga atggccgagt
    14041 agcaaaacag actcagccag aaaataataa taagtgctgc aacgggaagc ccccacagca
    14101 gcaccgggtg ttttatcgcg gtcatttcac aacggtaagc ggctgatggt ggaagatttt
    14161 taccgtgtca gcggcaatac gctctgcagc aaaaataccg cgcatccgcg cccaggtgtt
    14221 actgtcgacc gaagcaacct gctgcttctg cgcggcggtt aacatctgcc agagcggatc
    14281 ttgttgccag cgtttaacaa tgctctcttc gcgatagtgg gcaaccagca gccaggcagg
    14341 attgaccgcc agcagttgct ccaggccgat ggacggcatg gacgcacccg ccatcgcagc
    14401 gggaacgttc agccccagag aggccagcac gctgccggtc caggtctcct gagtatgcag
    14461 gttgaattgc tgttcgcgtg atgtgccaaa ggccacgcgt gtccctttgg gaagctggct
    14521 ggcccactgc gccatcctct ctttatgttg ttccaggcgt gcctgcatct ctcgcttttt
    14581 acccaccatt tcgccgatga tagccgcaga ttgcaaattt tcagcgtagg tttcgttgcg
    14641 ggacttaagc agcagtaccg gcgcgatttg ctgcaaggcg atgtaaaccc ccgcatggcg
    14701 actgctgtcg gcaatgatca ggtctggttt cagagcggca atggcttcca ggctcggctg
    14761 cgcgcgcgtt ccgacggact gccacggttt caggtgcgca cgcacttcgg gcaggatgcg
    14821 ttttgcatcg ttatcgtcgg caataccgat cgggctgacg tccacggcgg ccagcgcatc
    14881 ggcgaacgag agttccagca ccacaatccg ttgtggcgtt ttttcgagtg taaacgtgcc
    14941 gtgttcgtcc tgaaccgtgg cggcaaaggc gtggctgatc accagcagca ggcctgcaaa
    15001 aacaaaacgg ataaatgcca acataatcac attccagcta aaagcccggc aagccgggcg
    15061 ttaacacatc agaacttcaa cgacccctgc atatacagcg tgcgcggctg gcctgcatag
    15121 atgcctttgt tgttgtcgtc ataagagcgg atgaagtagt cctggtcgaa gatgtttttc
    15181 acaccgaatg ccaggttcag atctgccatc tgcgggccaa agtcatacgc cacgcgtgcg
    15241 ccccagagca tgaagccggg aatgcggccg gtactgccgt cggcgctctc tttcaccgta
    15301 ttggcgttat ccgcaaactg gctggactgg aaatcgctgt tcagattgaa cgtccagttt
    15361 cctggcttgt agtccacgcc cagcgtgcct ttatgtttcg gggagaatgg taccagattg
    15421 ccgtaggtgt cgcctttctc gcggatttcc gcgttcacat acgcatagct ggcgtagatg
    15481 gaaacgttat caagcgttgg cgttagcgta cccagatcgt aacgtgcctg cgtttccagc
    15541 ccggtatggc gcgttttgcc acgtgcagtg acggtgtcgt tggtctggtt ggagtcgtac
    15601 tgattgttaa agttaatcag gaacagcccc atttccgccg tcagcgcgcc gtcgtcgtag
    15661 cgggtaccga gttcccaggt tcgcgctttt tccggttcca cattgccgct ttgcacagcc
    15721 ttgccaatct ggctgtactg tacggtgccg aacgagcctt cagtgtttgc ataaagattc
    15781 cagctgtcag tcaggtgata gagcacgttc aacgccggaa gcggtgcgtt atagctcact
    15841 tcttcgtgcg tgcctgtgat ggcgttgttc tggtatgact cgatatgttc gaaacgcata
    15901 cccggcgtga tggtccagtt gccgatgtcg attttgtcat ccagatacca ggcgtgcgcc
    15961 tcggtgccgg aacgcgtatc gcggtcgtaa gggcttgagc cggacggcaa ctgcccgctg
    16021 ctggtggcgg tgtagtaacg catttcatgc gttgattcat tcacatagcg atagcccacg
    16081 cccacttcgt gcgcggaagg gccgatcata aagatctggc tgtagcgtgg ctcaataccg
    16141 cgcacccagt agttacgcgg cgagagggtg atgcgtttgc cttgctccag gtagccgctg
    16201 cgcagggttt gggtgtagaa cccctgaatg ttgaatttat gctggctgtc tggctggaac
    16261 tggtagccca ggctcgccag cttgcgacga ccccagaagc ggtcatacgg gcgggtggat
    16321 tgccagcgat cggcgtcgta atccgcgcga gacaggccac cgggcatgtc ggcttcaccg
    16381 tcgtaatatt gcagcaggct gttgaaggtg tgcacctcat ccggcgcata tttgcttttc
    16441 agcatcaggt cgtcgatgcg ggtggcgctg tgctcgcgcc agtcactgcc gcgcgtgccg
    16501 gagtagagca gcgcggtgcc aaaaccgttg tccgctgtgc cgcccaccat caggttgtgc
    16561 gtctctttcg ggttgttttg tgaagaggtt gggctgagct gaccttccac gcccgcctcg
    16621 ataccaaagt cctgcggaat ggcacgggta acaaagttca ccacgccgcc cacgctctgc
    16681 ggtccgtaac gcaccgcacc accaccgcgt accacgtcaa tggcatccat gttgccgagc
    16741 gaaacgggag ccagtgaaag ctgcggctga ccgtaagggg cgaaggggac ggggatgccg
    16801 tccatcagga cggtcgagcg gctggtgagg cgcgggttca ggccccggat gccaaagttc
    16861 atcgccaggt cgtggctgcc ggtgccgttg ttttccggcg cgctgacgcc agggatgcgg
    16921 ttaagtacct cacgcatggt ggttgcgccg gttttggcga aatcctcacg gcggatcacg
    16981 tcacgcgcgc cagcatgttc aaatacgtcg ttttcacgcg catcacccag ccagtcgccg
    17041 accacggtca gggcatcttc ttttggtgcg ggcgcgggct ccagcgtcca gctattattt
    17101 cccagcggtt ttacctgcag tccgctgccg tccagcagtt gttgcaggcc gctctcgacg
    17161 tcgtaatcgc cgtgcaggcc gttgctctgc ttgccgcgcg tcaggctggc gtcaaccgag
    17221 agggtaaatc cgctgtgtgc ggcatactga ttgagcgctt tatcgagcga tcccggtgcg
    17281 atattaacct gtgcagcaaa agcggaaaac gagagaccgg ccagcggcag caggctcagg
    17341 cgaatggcgt taaccaaagg tgttgtttta cgaaaaacgc gtaacggcgt cataccttcc
    17401 ccatcatcat ttttgttgtg ttcagctatg agtcgaacga gaagggtaaa aaggacaatc
    17461 gaaataagaa ttattttcct tacagtggtg aaatgtttat ccagtaccgc gtaatagact
    17521 gaattttaac gggaagcgtt tgcgcgataa cgttcaggat cgcatcggta tttttcagcg
    17581 ggaacgtccc gctcaggcgc agcccggcaa cggcgggatc gcagcgcagc acgccgttgc
    17641 ggtaacgggt tagcgtggct atcacctcac ccagtggttt atcgctgaag ctcaggatgt
    17701 ccttcgtcca gcttgtactc tcgtcatcca gcggtttcac tgcgccaaac tcagaggcgc
    17761 tgaactgcag gctttcacca gcgttcacga tgcgtttttg cgcgggggca ctggcgagaa
    17821 gcacttccac agcgtgctgc tgaacgtcaa gctgcgtgaa attatcctgc tggcggacgg
    17881 taaattctgt ccctaaagca gtgagctggc cctgacgggt caggacacgg aaggggcgtt
    17941 gcagggcatc tttcgcggta gtaatggcga tttcaccgta ccagagccgg acggtgcgct
    18001 gatgcgcatc aaaacgcaca tccgccgcgc tttgggtatt cagcgtgagc agggagccat
    18061 cttccagttg ctgacggctg actgtgcctt tggcggtgcg gtaatctgcc cgcagacctt
    18121 cgccggtttc cgactgccag agctgccagc ctccgccagc gccgagcaac agcagtaatc
    18181 ctttcatcac gtgacggcgg gtgaggcggg tatcgtgcaa cgcccggctg gcaacgtcgc
    18241 caggcacacc gccaagctgg ttgcgcaggt tttcaacctg ctgccaggcc cactggttat
    18301 cctgatcctg ttcataccac tgttgccagc gcgcttcctg ttgtgggctg acgcgctcgc
    18361 cgcttagcac ggcataccag tgggaagctg aacgcagcgc ctgacggcgg gaatcggtta
    18421 acaaaggatt cataacccat actccagacg gaacagcagg cagtgctcga cggctttcgc
    18481 cacgtatttt ttcacggagc tgatggaaac accgagtttg tgcgcaatct cgctgtatgt
    18541 cagaccatcc agttgcgaaa gcagaaacgc ttcacgtgtt ttgccgttta gcccgtccag
    18601 catgctgtcg aggagttgta gggtctcgag ttggctttcg cgttcctcag gtgaaggcgc
    18661 tcccccctcc ggcataagcg ccagcatctc cagatacgct ttttccagcg cgtttcggcg
    18721 aaacaggtcc accatcacgc gtttggcgat agtgcagagg aaggagcgag gatcgcggat
    18781 cgtcgagagc gtttcgctga ccattacccg caaaaaagtg tcctgggcaa tgtcatctgc
    18841 atcaaaagca gactggagtt tgcgcgtcag ccagcttttc aaccagccgt gatgtgtgcc
    18901 ataaagcgac tcgaacgtta aggaagctgt ggtagtggcg cggtcagaca tgcggagtgc
    18961 atcaaaagtt aattatcacg tagtcatatt aatatgagaa tggttatcat tacaattgga
    19021 aataaaattg tttccaatag acatttttaa catgttgttt ttctaagtgt tataaggtag
    19081 gtataaaatg ggatggagcc tctgcttctg gcatgtgtcg gtcagaatga ctcatgatgt
    19141 ggtctgctat tattgacatc ctcactgccc taaaggatgg ggatttcggt aatgctgcca
    19201 acttactgat ttagtgtatg atggtgattt taaggtgctt gcgtggcttc catttccatc
    19261 agatgtcctt cctgctccgc tactgaaggc gtggtgcgta acggcaaaag cactgccgga
    19321 catcagcgct atctctgctc tcattgccgt aaaacatggc aactacagtt cacttacacc
    19381 gcctctcagc ccggtacgca ccagaaaatc attgatatgg ccatgaatgg cgtcggatgt
    19441 cgcgccagtg cacgcattat gggcgttggc ctcaacacgg ttttacgtca cttaaaaaac
    19501 tcaggccgta gtcggtaacc tcgcgcatac aaccgggcag tgatgtgatt gtctgcgctg
    19561 aaatggacga acagtggggc tacgtcggtg ctaaatcacg tcagcgctgg ctgttttacg
    19621 cgtatgacag gatacggagg acggttgtgg cgcacgtctt cggtgaacgc actctggcca
    19681 cactggagcg tcttctgagc ctgctgtcgg cctttgaggt cgtggtatag atgacggatg
    19741 gctggccgct gtatgaatca cgcctgaaga gaaagctgca cgttatcagc aagcgttaca
    19801 ctcagcgcat tgagcgacat aatctgaatc tgagacaaca tctggcaagg ctgggacgga
    19861 agttactgtc gttctcaaaa tcggtggagt tgcatgacaa ggtcatcggg cattatctga
    19921 acataaaaca ctatcagtaa gttggagtca ttacctcctc ccgaactcaa agctatcata
    19981 cagataatta ctggttaacc ctatataggg cattttaaag tgttgtgatc ttacccagca
    20041 atagtggaca cgcggctaag tgagtaaact ctcagtcaga ggtgactcac aagacaaaac
    20101 agtttcaacc agtaaaaaaa cgtaaacacc attcgcatga atttcgtagt taagatctga
    20161 agcttgcaga atgcatcggt gttactgccg gagtccgtga acttagtctg catgaatcac
    20221 cacagcttta catctgtcgc agtaaacagc aaaatcagca aacttactta attatatcga
    20281 gtgcgattac aatcgctggc gatggtacag ttggtgtggc ggtctcagtc cggaacaatt
    20341 tgaaaatcag aatctcactt aaaacggcat ccattttaca aaagatggcc gtctgtgtat
    20401 ttataatgaa taccttctac atgtttagag tgcataatcc ac
//
LOCUS       DAMZET010000073        20148 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.073, whole genome shotgun sequence.
ACCESSION   DAMZET010000073 DAMZET010000000
VERSION     DAMZET010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.073"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..679)
                     /locus_tag="POB34_002675"
     CDS             complement(<1..679)
                     /locus_tag="POB34_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDI5919586.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLG"
     gene            complement(676..1566)
                     /gene="fhuD"
                     /locus_tag="POB34_002676"
     CDS             complement(676..1566)
                     /gene="fhuD"
                     /locus_tag="POB34_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HDI5919587.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            complement(1566..2363)
                     /gene="fhuC"
                     /locus_tag="POB34_002677"
     CDS             complement(1566..2363)
                     /gene="fhuC"
                     /locus_tag="POB34_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HDI5919588.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPTEGEILLDTQPLESWSSKAFARKVAYLPQQLPPAEGLTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            complement(2414..4603)
                     /gene="fhuA"
                     /locus_tag="POB34_002678"
     CDS             complement(2414..4603)
                     /gene="fhuA"
                     /locus_tag="POB34_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HDI5919589.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIANDGHTLNRGTVVDNERLQNFSVDTQLESKFATGEVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGNAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRVSYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            complement(4823..7345)
                     /gene="mrcB"
                     /locus_tag="POB34_002679"
     CDS             complement(4823..7345)
                     /gene="mrcB"
                     /locus_tag="POB34_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HDI5919590.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            complement(7541..9970)
                     /gene="hrpB"
                     /locus_tag="POB34_002680"
     CDS             complement(7541..9970)
                     /gene="hrpB"
                     /locus_tag="POB34_002680"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HDI5919591.1"
                     /translation="MSSLPVAAVLSELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKDLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            10044..10574
                     /gene="thpR"
                     /locus_tag="POB34_002681"
     CDS             10044..10574
                     /gene="thpR"
                     /locus_tag="POB34_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HDI5919592.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            10589..11293
                     /gene="sfsA"
                     /locus_tag="POB34_002682"
     CDS             10589..11293
                     /gene="sfsA"
                     /locus_tag="POB34_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDI5919593.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            11471..11926
                     /gene="dksA"
                     /locus_tag="POB34_002683"
     CDS             11471..11926
                     /gene="dksA"
                     /locus_tag="POB34_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HDI5919594.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            11963..12889
                     /gene="gluQRS"
                     /locus_tag="POB34_002684"
     CDS             11963..12889
                     /gene="gluQRS"
                     /locus_tag="POB34_002684"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HDI5919595.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWRLTAVPESAIVNSTFSNASC"
     gene            12928..14346
                     /gene="pcnB"
                     /locus_tag="POB34_002685"
     CDS             12928..14346
                     /gene="pcnB"
                     /locus_tag="POB34_002685"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HDI5919596.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYDTYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            14343..14822
                     /gene="folK"
                     /locus_tag="POB34_002686"
     CDS             14343..14822
                     /gene="folK"
                     /locus_tag="POB34_002686"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDI5919597.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            15192..15776
                     /locus_tag="POB34_002687"
     CDS             15192..15776
                     /locus_tag="POB34_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI5919598.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            15881..16621
                     /locus_tag="POB34_002688"
     CDS             15881..16621
                     /locus_tag="POB34_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDI5919599.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            16656..19256
                     /gene="htrE"
                     /locus_tag="POB34_002689"
     CDS             16656..19256
                     /gene="htrE"
                     /locus_tag="POB34_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HDI5919600.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RLSYSVNTGYTMNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIE"
     gene            19273..19842
                     /locus_tag="POB34_002690"
     CDS             19273..19842
                     /locus_tag="POB34_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI5919601.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            19857..>20148
                     /locus_tag="POB34_002691"
     CDS             19857..>20148
                     /locus_tag="POB34_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein StaE"
                     /protein_id="HDI5919602.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIK"
BASE COUNT         5121 a         5226 c         4871 g         4930 t
ORIGIN      
        1 gcccgagatt gcgcgccacg ccatcatcaa gccccatcag ggttaacggg cgaagtagca
       61 ataacgtcaa catcacgccg cccagcaact gcggccataa acgctcaacg ccgccccagt
      121 cggtttgcgt cagcgttccg gtgctccaca ggaacatgct ttgcagctgg tcgtgatgga
      181 agataaccag tagctgatta attgccccgc aataaaggct caccaccagc cccgcgagaa
      241 taagcgtaac cggcgaaagc cgtttccccc acgcgacgcc aaagacgatt aagccaacga
      301 cacaagcgcc caccagtgcg gcaaactggc tcgccatagc acctgggatc gcccaaagtg
      361 tggtgacggt aattcccagt tgcgcgcctg tagcaacgcc aagtgtcgtc ggttccgcca
      421 gcggattacg cagcacttgc tgaaacagca cgcccaccag ccccaggcct gcgcccacca
      481 gcagcgaaat cgccagacgc ggcaacaagc tgtagtgaaa aatcatctgc tcgatgacgt
      541 caatatccgg cgaccaggcg gcctgcgccc actgactgcg cggcagcgcc tgtgagaagt
      601 tcatccaggt tagcgccgta gcgacaatca ctaacagcgc cagcaataac gccgggaaaa
      661 gcgcaattcg tttactcacg ctttacctcc gatggcgtta tccagaacac gcacaaagtg
      721 cattgccgaa agcgttgcac cataaaacca gactgcgggt acgcgctgaa agcgccctgt
      781 acggacaaac ggcatggcct gccacagcgg ggttgccatt agcgcatcca tgtctttgct
      841 gttgtcgtga tcaaaacaga gcacatcaac gtctttatac gccgccagac gatcgatact
      901 gacggcggta ctgccccaga agttggtttc cccttgccag gcatttggga tgccgtactc
      961 atcgagaatt tcctggaaca agctgtttgg accgaagacc agcatatggc gcggatcgat
     1021 aagcgtcgtc agcagtaacg ggcgtgcacc acgcttcaca aagcggggtt tcatgctgcg
     1081 gataaagtct tcgtattgcg ctaaatgcgt ttccgctgcg ctttgcaggt taagtaaatc
     1141 tgccatttcc gtcagcgatt tacgcgccat cgccaacggc tgtttgccgt cactgaagtt
     1201 aaatccgcga cccggcgcaa tacgtgccag catttctgat gaagggccat atcctgccga
     1261 ccagaccata aacgacggtt tcatttcggt cagcagttca aggttaggtt ctgtacgcaa
     1321 accgacgtca atcactgagt ccggcaatgg tggttcgctg acccacaggc gatagttgat
     1381 ggtatccgcc acgccgtaag gcacgatgcc gagcgccagc agtaattcca ccggcaacca
     1441 ctccagcgcc acaatacgat tggggtcaat agccgccgcg tgggcggtat tcatctgcca
     1501 taacaacgga gaaagcgcca tcgccgttaa cagtcgacgg cgcgaaataa gaggtaagcc
     1561 gctcatcaat aaacaaaact cacaggtgca gcacccgccg gatgcggcaa aatacccatc
     1621 gggatgccat aaatcatttc gagggtttcg ccgcgcataa tttccgcagg cgttccctga
     1681 gcaatcattt caccaccgcg cagggcgacc agataatcac agtagcgtgc cgccatattg
     1741 atatcgtgca gcacggcaat gaccgtcagg ccacgttcct gacttaaacg gtgcaccagc
     1801 gccagcacat caacctggtg ggcgatatcc agcgccgaag tcggttcgtc gagcaacaga
     1861 caacggctat cctgcgccac cagcatggcg atccacgccc gttgacgttc gccgccagag
     1921 agactatcga ccagccgatg cgccagcggt tttaagccaa ccagcgagat agcttcctcg
     1981 actttttcgc gatctgccgc cccaaagcgc cccagcgcgc catgccacgg gtaacgacca
     2041 atcgccacca gttcacgcac ggtcaaccct tctgccggag gaagctgctg cggcaaataa
     2101 gccactttgc gggcgaacgc tttgctgctc cagctttcca gcggctgggt atcaagaaga
     2161 atctcccctt ccgtcggagg ctgatgacgg ccaagcattt tgagcagagt ggatttacca
     2221 gaaccgttgt gaccgatcag accggtcact ttcccggcag gaaaggttaa cgacagcgga
     2281 tgcaaaagcg tgcgcccggg cacacgaaag gagatattac gcagtgcaaa agtggtatcg
     2341 gaatgattcg tgtattcctg cataacagcc aacttgtgaa atgggcacgg taatccgtgc
     2401 cccaaaagag aaattagaaa cggaaggttg cggttgcaac gacctgacgt tctgcgcccc
     2461 agaagcagcc ataagtctgg aagcaactgg caacatactc acgatcgagc aaattgttga
     2521 cgttcacagc aaggctggat cccgccatac caaagcgtgc cagatcatat tttacaacag
     2581 catccattac agctgcgcta cccactttaa aagtgtttgc cggatcacca tagctggaac
     2641 caataaaacg accacctgtt cccaatgtta aaccagaaag cggcccttca ttgaaggtat
     2701 aatcccccca gagagatgcc atatgctccg gtacttgttc tggagtttta cctttgagat
     2761 tggtgtcttt ggtgtattcc gcatccgtgt aggtataaga agccgtcaag ttaatattgg
     2821 catttaacgc tgcttttgct tcaagctcca caccacgagc gcgaatctca cctgctggga
     2881 cttgcgctaa cgggtttgtt gggtctgctg tcaggttatt tgttttcgtc aattgataga
     2941 ctgcgcccgt aacaacgacc ggcatatcat tcggaacata ttttacgcca gcttcatact
     3001 gttcaccttt cgatggctta taggaaacgc gcgggttgct ccacagatcg aaagcactcg
     3061 gttcaaagga ctggctatag ctaaagtaag gtgaaatacc attatcgaat aagtaattta
     3121 caccaccgcg ccaggtgaac tgatgatcat tacgctcaat ataaccttca gtcggcgtat
     3181 aagagttttc acgaacagtg gtcgcttgct tagaccaatc gtagcgtccc cccagagtga
     3241 atacccattt attccattcc gcctgatcct gaacataaat acctgtttgt ttgctttcat
     3301 tcatttggta tggctctgcg ttaccaaatg caaagtattc aggatgatat ttgttataaa
     3361 gatcgattga tggtgcagat ccaaagctgg cattgatatc attgcgcata cgcatgaagt
     3421 ctacccctgt cagcaaagta tgctccactt cacctgtagc aaatttactt tcaagttggg
     3481 tatcaacgct aaagttttgc agacgctcat tatccaccac tgtcccgcgg tttagagtat
     3541 gaccatcgtt ggcaatccct gtgccataaa cacttttctg tgaggttttc atttcactga
     3601 aacgcagatt ctgacgcacg gtgaaggtgt cattaaaacc atgttcaaaa ctatatccca
     3661 ccattttctg gttacgggag tatgtattat tcgatgcacc ttcattgaag tcagtcggta
     3721 gacgtttacc attaggcaat ggctcaacgg tcccctcttt cggcaaccaa ccgtaataac
     3781 ccgtttctgg ttcattctgg aaataggaca ggaaagtaaa attggttttg tcgtctggac
     3841 gccatgagaa tgatggagca atggtataac gctgggattc agaatttttc tgctgttcgt
     3901 ttgttgaacg agcgaggccc gtcaaacggt aggagaactc accgttatca tccagtgcat
     3961 cactgaaatc gaatccagtc tgaaacagat tatccgtccc cattttaaac tgaatttctt
     4021 tcaggggttc agtggtcgga cgcttactca ccatagaaat aatcccacca ggattacttt
     4081 ttccatagag aacagacgtc ggcccacgca tcagttcaac cctctcgagc atgtaaggat
     4141 caatcacagc atcgttataa aagttgccct gaagttttag tccatcaagg taattatttt
     4201 gattcagacc aacagatgag aaaccacgaa tgataacaaa atcatatgta ttagaagcac
     4261 cacgactgct aaccgtaacg ccaggtgtat aacctaatgc ttcttttaca gactgaaatt
     4321 gatgcatctg catctcttca tttgtcacaa ccgaaataga ctgcggcgtt ttttcaattg
     4381 gtgtatccgt tttagtcgca gtagcagagt gcttcgccgc aatagttgca gcaggccccc
     4441 atgcgctttc ttgcggcgca ggtgcagcgg taacggtgat agtgtcttct ttcggttcaa
     4501 ccgctgcctg tgcataaaca gacatgccgc taaccgctgt ggctactaca actgcgattt
     4561 tacgcagtga gtgttttggc tgagcagttt tggaacgcgc cattggtata tctctgatgt
     4621 aaagtgaatg ataacgtaaa cgagaatgat tattataaag ataagataat attcgaaacg
     4681 ctggcgggtt cgcaagcggt acgcaatctg agcgttcctg aagggttaat aaacaaccag
     4741 atgaaaagaa agggttaata ctttaggtgg gatgttattt taccggatgg caattcgcca
     4801 tccggtattt cacgcttaga tgttaattac taccaaacat atccttgatc caaccggcta
     4861 caccgtcgct gtctttctgc tcttgctgag caggttgctg ttgcggctga gaagactgat
     4921 caaacggatt gcctgacggt tgctgctgca tctcactctg ctggcacagc gattgcggat
     4981 cgctggtcca gaccggcaag acacgcatgc caccgctgca aacaaagttg ccgtcgtagt
     5041 ccacgcccat atctgcaata tcttctggcg gaacaagatt cagcggcgtt ggcgtctggt
     5101 tagccagata acgctgataa atcgacattg ccccgctggc accatacagt ttggtcggct
     5161 ggttgttatc acggcctacc caggtgatgg tcaccgtgct gccgtcaatg cccgcaaacc
     5221 aggtatctac gttattgttg gtagtccctg ttttccctgc cagatgcagg ttcgggtatt
     5281 tcgccccaag ctgacgaccc gtaccgcgtt gtaccacctg ctgcatggtc catagtgtca
     5341 gatacgccgc ctgcgccgga acagcgcgtt ccgcctgcgg gaagctctga tacagcacct
     5401 tgccatcttc cgcaattacc gaacgcagtg cagaaagcgg tgcacggtta ccgccgctgg
     5461 cgatggtctg gaacgcctgc gccacttcga ttggcgttaa gttcaacgcc cccagcagca
     5521 tcgccggaac cggatgcaac tgatctttcg gtacgcccag tttgatccag gtctccgtaa
     5581 ccgcaggcag ccccagcgcc atccccagat ttaccgtcgg cacgttcatc gaacgggtca
     5641 acgcatccac cagcatcact ctgccgcttt cgctataacg acggtcatca ttctgcggtg
     5701 accagacctg gccattcggc tgacgcagcg cgattggcgc atccgcaatc cacgtattca
     5761 gacgatagat tttcggctgg cttaaggcag tcagataagt cgctggtttt gcaagggaac
     5821 caatcgaacg acgcgcctgc atcgcacggt tgtagcccgc aaactgcggc tcagaaccac
     5881 cgaccatcgc acgaacttca ccactaaagc ggtcgacgac cacaatcgca gtttcaagat
     5941 cgctcaactt acgctgtttc ttcagtgccg gaatgccttc cacggcggct ttttctgccg
     6001 cgtcctgggc caccgagtca aaggtagtga agatcttcac gccggagaga tcttttacct
     6061 tatcgcctaa tttcgcctgc agctcctgac gcaccagttg cataaaggct ggctgtggag
     6121 agatcacccc accgcgtggc tgaacaccca gcggacgtgc actcaacatg tcatagagtt
     6181 cttgatcaat aatctgttgc tgttgcagca gacgcagcac cagattacgt cgctccagcg
     6241 ccagttttgg gttacgccac gggttgtaga tggacgctcc tttcaccatg ccgactaaca
     6301 gcgcctgctg gtcgaggctt agctcttcta ccgggcgacc aaagtaatac aagctcgcca
     6361 gcgggaagcc gcggatttcg ttgtcgccgc tctgaccgag atacacctcg ttcatataca
     6421 gctcaagaat acggtctttg ctgtaacgcg cgtccatgat cagcgccatg taagcttcgt
     6481 tcgctttacg ccagtaagaa cgctcactgg agaggaacag gtttttcacc agctgttgcg
     6541 tcagcgtact cgctccctgc accgtgcgtc cggcggtcag gtttgccagc accgcacgtc
     6601 cgatggagta gaggctgatg ccgtcatgct cgtaaaaatg acggtcttct gtcgccagca
     6661 aagtatccac cagcaaatca gggaaaccac tgcgcggcac aaacaaacgc tgctcaccgt
     6721 ttggcgagga gatcatggtg atcagacgcg gatcaagacg gaagaaaccg aactgacggt
     6781 tgttctccat attgacgatc gtcgccagat gatcgccatc aaaggtcaga cgcgcacgca
     6841 cctgcccttc tttactgtcc gggaaatcaa acggacggcg gatcatctca atgctgttgg
     6901 cctgcacggt aaattcgcca ggacgggtca ttttcgacac ctgacgatac tgagtcgcct
     6961 ccagcagctt caccatctcg ttcttgctga tggtcatatc tggctcaagg ttgaccattc
     7021 ggccataaac tgccgcaggc agttgccaga ccttgccatc aatacggcta cgaatttttt
     7081 gatcgagata aacgccgtaa atggcgatca gcacggcaaa aacgatagcc agttttaaca
     7141 gcagccatag ccagccgcgt ttgccacgag gcttacgccc tttgcctttg cccttacctt
     7201 tgcgcggcat cggttcttca tcctcatagt catcataatc gtcgtaatca tcgtcatctt
     7261 cgtaacgacg acggcttacc ttttgtttga ccggacgcgt cggtttccct ttgcgtccaa
     7321 ttggctcgcg gtcattcccg gccatgcttt ttctccgcaa tattcaggcg caaaagcccg
     7381 attttctgtt cttctgttac aagacagaag aagatatctc tcaaaatttg tgctcgatcg
     7441 gtcccctctc ccttgaggga gagggttagg gtgagggtga acgtgcgcgc tgatgccctc
     7501 accccggccc tctcccacag ggagagggag aaaaccaacc ttacgaatac tttttcgtcc
     7561 gtcgcgtcgg tgcagtattt gccgggtcgt ccggccagac atgtttggga taacgccctt
     7621 tcatctcttt ttgcacctca cggtacgctc ctttccagaa ggcgctcaaa tctcgtgtga
     7681 tttgtaatgg cctttgggca ggtgaaagca actccagcac cagcggcacg cgcccctggg
     7741 cgatcgtcgg attggtggcc tcgccaaaca tctcttgcat tctcaccgcc agcgcgggcg
     7801 ggttatcttc atgataacga atggcgatcc ggcttcccgt cggcacagtg taatgcgcag
     7861 gcaattcact atccagacgt tgctgcattc cccaatcaag taatccgcgt agtgcctgat
     7921 aaatgtcgag tgatttcagg ccgcgtagtg aatgtacgcc agtcatatgt ggcagcagcc
     7981 acgtttccag cgtcgccaat aaactttcat catcaaccgc tggccagtca tattccggca
     8041 accactttgc ggcgcataac aaacgcaagc gtagctgttc cgcttccgcc gtccagttga
     8101 gcacgcttaa atctttatcg cggatgccat taagcatcgc ctgatgcaac tcgtcttccg
     8161 agggtttcgc cagcggctgc actttcaccg tcagcagacc gatttgcagc cgacgccagg
     8221 ctttcagcgt accttgcgcg tcatcccact ccacagtgtc agactgctgt accagctgcg
     8281 ggcagcgttg tactaactca tcaatatcga ccggcagcgc cagtaaaatc cgcgcatccg
     8341 gcgaggcgct gccctgcaat aataacggtg cgatcaacca ttcgtggcgg cttagcgcgt
     8401 cgttggcatc gagcatcgct cccatgccgt ttgccagttg atagcgtcca tcttgcccac
     8461 ggcgacgagc aatgcgatcg gcaaacgccc cggcaagtag cggcgcgata agcgaactgt
     8521 ctgcctcacc gccacgtacg tttaagcgtt ttaacagttg ctgactacgt tgctgccagg
     8581 ctggttgatt gcgcgaaaac gccacgccca ggtcactatt gcccatccgt ggcggctctt
     8641 cgagaatggc ggcaattttt gccgcggtag cagcttcgtc gtcgttcttc gcgctaacca
     8701 gcattgccgc taaacgcgga tcgttaccca gcgctgccat tttttgccct tgcgcactaa
     8761 gccgttcacc ctccagtgcc cccagcattt gtaacagacg tttcgcggcc agtagattca
     8821 ctactggcgg ttgatccagc cagctcatct gcgccggatc gctgcatccc cattgcagta
     8881 attccatcag caaaccggaa agatcgcttt gtaagatctc cggttcactt tgcgccgcgg
     8941 cgcgttctgc ttgttctttg gcgattaaat gcaggcagat acccggctcc agacgcccgg
     9001 cgcgcccggc acgctgcgtc atggatgcct ggctaacgcg ttgagtaatc agtcgcgtaa
     9061 gccccgtgcg cggatcaaaa cgcgccacac gctcctgggc acaatccacc accagacgaa
     9121 taccttcaat ggttaaactg gtttcagcaa tattggtcgc cagcaccact ttgcgcatcc
     9181 cttgcggtgc cgggaggatc gcttttcgct gatcgttcag cgacaacgcg ccatacagcg
     9241 ggcagagcaa tacatcactg ccgatgcgcg aagccagttg ttcctgcaca cgctgaattt
     9301 ctccgacgcc aggtaaaaat aacagtaatg atccgctttc ctgacgcagc atttcagcgg
     9361 tggctaccgc aacggcatcg tcaaaacgct gatgcgcggg cagcggtaaa taacggcgtt
     9421 caaccggaaa cgagcgccct tctgagatga cgacaggcgc ttctggcagc atttgctgca
     9481 agcggtcgtt gtccagggta gccgacataa tcagcagttt aaggtcatca cgcagacctt
     9541 gttgcacatc gagtaacagc gccaacgcca aatccgcctg caagctgcgc tcatgaaatt
     9601 catcaaggat caccagtcca acaccgctca gttccgggtc acgctggatc atgcgcgtca
     9661 gcacgccttc ggtaaccact tccaggcggg tattcggccc gacgcagttt tgcgcacgca
     9721 tccggtagcc aacggtatcg cctggctttt cgttaagcag ctccgccagc cgttgcgcga
     9781 cgttacgcgc cgccagacga cgcggctcca gcaggataat tttcccgtta atgccgggat
     9841 gcgccagcag ttgcagcggc agccaggttg atttcccggc cccggtcggc gcacttaata
     9901 atacctgcgg cgcacaatcg agagcggtaa gtaattcaga taagacggca gcaacgggca
     9961 acgacgacac aaaacgctcc agagggttaa cattcttcgc gccacattgt agcatcgcgg
    10021 taattcataa ccgagtgcct cacatgtctg aaccgcaacg tctgttcttt gctatcgact
    10081 tacctgcaga aatccgcgaa cagattatcc attggcgcgc cacacacttc ccacctgagg
    10141 cgggacgtcc ggtcgccgcc gataatttgc atctgactct ggcattttta ggcgaagtga
    10201 gcgccgagaa agagaaggcg ctttctcttt tagccggacg gattcgtcaa cctggtttca
    10261 cactcacgct tgatgacgcc ggacaatggc tgcgttcgcg tgtggtgtgg ttagggatgc
    10321 gtcagcctcc acgcggctta atccagctgg cgaatatgct ccgttcacag gctgcccgca
    10381 gcggttgttt tcaaagcaat cgtccgtttc atccacatat taccttattg cgcgacgcca
    10441 gcgaggcggt gacaatcccg ccgccaggtt ttaactggtc ttatgcggtg acggagttca
    10501 ccctttacgc ctcctcgttt gcccgtggac gcacacgcta cacgccgcta aaacgctggg
    10561 cgctaacgca ataacaagga ttgtcgcaat ggaattctct ccccctctac agcgcgcgac
    10621 gctaattcag cgttacaaac gttttttagc cgatgtgatc acacccgatg gtcgcgaatt
    10681 aacgctacac tgcccgaata cgggtgcaat gaccggttgt gcaacgcctg gcgataccgt
    10741 ctggtattcg acttcagaca acaccaaacg gaaataccca cacacctggg aattaactca
    10801 aagccagagc ggcgcattta tttgcgtcaa cacgctttgg gctaacaggt tgacgaaaga
    10861 ggctatcctt aatgaatcaa tttcagaact gtcaggctat agctcgctga aaagcgaagt
    10921 aaaatacggc gcagaacgca gccgtattga ctttatgttg caggcggatt cgcgtccaga
    10981 ctgctatatt gaagtgaaat cggttacgtt agcggagaac gaacagggat attttcccga
    11041 tgcggtcact gaacgaggtc agaaacacct tcgggagttg atgagcgtag cggctgaagg
    11101 ccagcgtgcg gttatctttt tcgccgtgct gcattcagcc attacacggt tttcacccgc
    11161 gcgccacatc gatgagaaat acgcgcaact attgtcagaa gctcaacaga ggggggtaga
    11221 aattctggct tacaaagcgg aaatttctgc tgaaggcatg gctcttaaaa aatcactgcc
    11281 ggttacattg tagtaaagta agtaactggt taatttacat tctggtcgcg tgcgcaaata
    11341 cgcttttcct cacacagttg tcaagtgtta cgtttagata attgctatcc ggaaaagcat
    11401 ctgctattta tagcggcctc atttttcccc cgaacatggg gatcgatagt gcgtgttaag
    11461 gagaagcaac atgcaagaag ggcaaaaccg taaaacatcg tccctgagta ttctcgccat
    11521 cgctggggtg gaaccatatc aggagaagcc gggcgaagag tatatgaatg aagcccagct
    11581 ggcgcacttc cgtcgtattc tggaagcatg gcgtaatcaa ctcagggatg aagtcgatcg
    11641 caccgttaca catatgcagg atgaagcagc caacttcccg gacccggtag accgtgcagc
    11701 ccaggaagaa gagttcagcc tcgaactgcg taaccgcgat cgcgagcgta agctgatcaa
    11761 aaagatcgag aagacgctga aaaaagtgga agacgaagat ttcggctact gcgaatcctg
    11821 cggtgttgaa attggtattc gccgtctgga agcgcgcccg acagccgatc tgtgcatcga
    11881 ctgcaaaacg ctggctgaaa ttcgcgaaaa acagatggct ggctaattac agccgttcca
    11941 tcacgtttac cacaggcggg aaatgctccc gccttatttt ttgttcaaag agatgacaga
    12001 cacacagtat attggccgct tcgccccctc tccttccggc gagcttcatt ttggctctct
    12061 gatcgccgcg ctcggcagct atttgcaggc tcgcgcccgg caaggtcgct ggctggtacg
    12121 catagaagat atcgacccgc ctcgtgaagt tcccggtgcc gcagaaacta tcctgcgcca
    12181 gctggaacat tacggtctgc actgggacgg cgatgttctc tggcaatcgc aacgtcacga
    12241 cgcctatcgt gaagcactcg cctggttaca tgaacaagga ctaagttatt actgcacctg
    12301 tacgcgtgcg cgtattcaaa gcattggcgg tatttacgac ggtcattgcc gggtgttgca
    12361 tcatggacca gacaacgccg cagtgcgtat ccgccagcag catccggtca cgcaatttac
    12421 tgaccagctg cgcggcatta ttcacgccga cgaaaaactg gcacgggaag attttatcat
    12481 tcatcgccgt gatgggttgt tcgcctacaa cctggctgtt gtggttgatg atcatttcca
    12541 gggcgttaca gaaatagtgc gtggggctga tctgattgaa ccaacagtaa ggcaaatctc
    12601 gctgtaccag ctttttggct ggaaagtgcc agattacatt catctgccgc tggcgcttaa
    12661 tccacaaggc gctaaacttt ccaagcagaa tcatgcgcct gcgttgccga aaggcgatcc
    12721 acgcccggta ctaatcgcgg cacttcaatt tctggggcag caggcagaag cacactggca
    12781 ggatttcagc gtcgagcaaa tccttcagtc agccgtcaaa aactggcggc ttaccgccgt
    12841 gcccgagtcg gcaattgtaa attcaacatt ctcaaatgcg tcatgctgag ctatgattag
    12901 ccgctatttt tttgtcctga atgatgtttg acactaccga ggtgtactat ttttacccga
    12961 gtcgctaatt tttgccgcaa ggtgctaagc cgcgaggaaa gcgaggctga acaggcagtc
    13021 gcccgtccac aggtgacggt gatcccgcgt gagcagcatg ctatttcccg caaagatatc
    13081 agtgaaaatg ccctgaaggt aatgtacagg ctcaacaaag cgggatacga agcctggctg
    13141 gttggcggcg gcgtgcgcga cctgttactt ggcaaaaagc cgaaagattt tgacgtaacc
    13201 actaacgcca cgcctgagca ggtgcgcaaa ctgttccgta actgccgcct ggtgggtcgc
    13261 cgtttccgtc tggctcatgt aatgtttggc ccggagatta tcgaagttgc gaccttccgt
    13321 ggacaccacg aaggtaacgt cagcgaccgc acgacctccc aacgcgggca aaacggcatg
    13381 ttgctgcgcg acaacatttt cggctccatc gaagaagacg cccagcgccg cgatttcact
    13441 atcaacagcc tctattacag cgtagcggat tttaccgtcc gtgattacgt tggcggtatg
    13501 aaggatctga aggacggcgt tatccgtctg attggtaacc cggaaacgcg ctaccgtgaa
    13561 gatccggtac gtatgctgcg cgcggtacgt tttgccgcca aattgggtat gcgcatcagc
    13621 ccggaaaccg cagaaccgat ccctcgcctc gctaccctgt tgaacgatat cccaccggca
    13681 cgcctgtttg aagaatcgct taaactgcta caagcgggct acggttacga tacctataag
    13741 ctgttgtgtg aatatcatct gttccagccg ctgttcccga ccattacccg ctacttcacg
    13801 gaaaatggcg acagcccgat ggagcggatc attgaacagg tgctgaagaa taccgatacg
    13861 cgtatccata acgatatgcg cgtgaacccg gcgttcctgt ttgccgccat gttctggtac
    13921 ccactgctgg agacggcaca gaagattgcc caggaaagcg gcctgaccta tcacgacgct
    13981 ttcgcgctgg cgatgaacga cgtgctggac gaagcctgcc gttcactggc aatcccgaaa
    14041 cgtctgacga cgttaacccg cgatatctgg cagttgcagt tgcgtatgtc ccgtcgtcag
    14101 ggtaaacgcg catggaaact gctggagcat cctaagttcc gtgcggctta tgacctgttg
    14161 gccttgcgag ctgaagttga acgtaacgct gaactgcagc gtctggtgaa atggtggggt
    14221 gagttccagg tttccgcgcc accagatcaa aaagggatgc ttaacgagtt ggatgaagag
    14281 ccgtcaccgc gtcgccgtac tcgtcgtcca cgcaaacgcg caccgcgtcg tgagggtacc
    14341 gcatgacagt ggcgtatatt gccataggca gcaatctggc ctctccgctg gagcaggtca
    14401 atgctgccct gaaagcatta ggcgatatcc ctgaaagcca cattcttgcc gtttcttcgt
    14461 tttaccgcac cccaccgctg gggccgcaag atcaacccga ttacctcaac gccgccgtgg
    14521 cgcttgaaac ttcgctcgcc ccggaagagt tactcaacca tacacagcgc atagaactgc
    14581 aacagggtcg tgttcgtaaa gctgaacgct ggggaccacg cacgctggat ctcgatatca
    14641 tgctgtttgg taatgaagtg ataaataccg aacgcctgac cgttccgcac tacgatatga
    14701 agaatcgtgg atttatgctg tggccgctgt ttgagattgc accggagctg gcgtttcctg
    14761 atggggagac gttgcgtgaa gtcttacata caagagcatt tgacaaatta agcaaatggt
    14821 aatttattta tattacgaat ataaatattt tctgaaaatc atacatcctc tctttcctta
    14881 ataaaaattt tagtttaaac atttttgcag aaacataata taaattcccg cagataaata
    14941 tcaatcccat cgccataccc taaccaattg cacttgtgaa tttatttttg cttaattttg
    15001 aattaagaaa atttaatttg ttcaaaagcc cttaaagtgc acaataggcg tcctaatgaa
    15061 caatggttgt aaattttggt aacaaatatc attgctcatt aatatctagc atctgcttat
    15121 aacatttgtg tcatgaatag tcattcttca aaccagtagt ctgactaatg gaattttatt
    15181 caaggatttt tatgtctaaa aaattaggtt ttgccctgtc tggtcttatg ctggcaatgg
    15241 ttgcaggtac tgcttctgct gatatggatg gtggtcagtt aaatatcagt ggtctggttg
    15301 ttgataacac ctgtgaaacc cgcgttgacg gcggtaataa agatggtctg atcctgctgc
    15361 aaactgcaac tgttggtgaa atcgatgctg gcgtgctgaa tgacaccgtt ggcgctaaag
    15421 ccaaaccttt cagcatcacc gttgactgca gcaaagcaaa tccgaatcca ggtagtacag
    15481 ctaaaatgac cttcggttct gttttctttg gtaatagcaa aggaacgctg aacaacgaca
    15541 tgtctattaa caacccgtct gacggcgtga atatcgctct gcacaatatt gatggttcca
    15601 ctatcaaaca ggttcaaatt aacaaccctg gcgatgtgta tactaaagcc ctggatgcaa
    15661 cgacaaaatc tgctgtttat gattttaaag cgtcttacgt tcgtgccgtc gcagaccaaa
    15721 cagcaactgc tggttatgta aaaactaaca ctgcatacac cattacttat cagtaagtat
    15781 tcaccaatta aattgattga atgaatatac agggaatagt gttaataatt tctattttta
    15841 tattattccc tgttttaatt aactctgtca gggatggttt atgtttttta acaccaaaca
    15901 tacaacagct ttatgcttcg taacctgtat ggcttttagt tcatcgtcta ttgcggacat
    15961 tgtcatttcg ggtactcgcg taatatataa aagcgatcaa aaaagtgtca acgtacgtct
    16021 ggaaaataaa gggaataacc cgttgcttgt ccagagttgg ttagatactg gcgatgacaa
    16081 cgctgagcct ggcagtatta cagtcccttt tactgccacg ccgccagtat cgcgtattga
    16141 tgccaaacgt gggcaaacaa tcaaattaat gtacacagcc agcacctcac tgcctaaaga
    16201 cagagagagc gtgttctggt ttaacgtact ggaagttcca ccaaaaccag atgcagaaaa
    16261 ggtcgcgaat caaagtctgc tgcaactggc atttcgcaca cgtataaaac ttttctatcg
    16321 cccggatgga ttgaagggaa atccctctga agccccgtta gccctgaagt ggttctggtc
    16381 aggttcagaa ggtaaggcgt cattgcgagt gaccaatcca accccttact acgtctcttt
    16441 tagcagtggt gatttagaag ctagcggtaa acgctatccg attgatgtga aaatgattgc
    16501 accatttagt gatgaggtca tgaaagtcac tggccttaat ggcaaagcaa gttctgcaaa
    16561 agtacatttt tacgccatta atgattttgg tggcgccatt gaaggtaatg ccagtctgta
    16621 atcaggcaag gatataattc cgcaggaagc ataacgtgac tataaaacca actaaaaatt
    16681 atcataatca tctgacccgt atcgctacgt tttgcgcgct gctgtattgc aatagtgctt
    16741 tctgtgctga acttgttgaa tatgaccata ccttcctgat ggggcagaat gcctcaaata
    16801 ttgatctcag ccggtatagt gaaggtaacc ctgctatacc gggtatgtac gacgtcagtg
    16861 tttatgtcaa cgatcaacct attattaacc aaagcattac atttattgaa attgaaggaa
    16921 aaaaaaacgc acaagcctgt atcacactta agaatttatt gcagtttcat ataaattctc
    16981 ccgatataaa taacgaaaaa gccgttctgc ttgccaggga tgaaacactc ggcaattgcc
    17041 ttaatttgac ggaaattatc cctcaggctt ctgttcgtta tgacgttaac gagcaacgtc
    17101 tggatataga cgttcctcag gcatgggtaa tgaaaaatta ccaaaactat gttgatccat
    17161 cgttatggga aaatggcatt aatgcggcca tgttgtcata caacctcaac ggatatcata
    17221 gtgaaactcc tggtcgaaga aatgacagca tttatgctgc ttttaacggt gggatgaatt
    17281 taggtgcatg gcgactgcgt gcctcaggca actacaactg gatgaccgat tctggcagta
    17341 attatgattt taagaatcgg tatatacagc gtgatatcgc ctcgctgcgt tctcaactca
    17401 ttcttggtga gtcttataca acgggcgaaa cctttgattc tgtcagtatc cgcggcattc
    17461 gtttatacag cgacagccgc atgttgcctc cgacattagc gagttttgcg cctatcattc
    17521 atggcgttgc taataccaac gccaaagtca ctattacgca aggtggctat aagatttatg
    17581 aaacgactgt gccgccaggc gctttcgtca ttgatgatct gagtccgtca ggttacggca
    17641 gcgatcttat tgttactgtc gaagaatccg atggctcaaa gcggacattc tcgcaacctt
    17701 tttcatcggt cgttcaaatg ttacgccctg gcgttggacg ttgggatatt agcggcggcc
    17761 aggtcttaaa agatgatatt caggatgagc ctaatttatt tcaggccagc tactactacg
    17821 gtctgaataa ctatctaacg ggttatactg gtattcagat taccgataat aactataccg
    17881 ctggtttgtt aggtcttggt ctgaatactt cagtcggtgc attttctttc gatgtgactc
    17941 actccaatgt tcgtataccg gatgataaaa catatcaggg acaaagttat cgtgtttcct
    18001 ggaacaagtt attcgaagaa acaagtactt cactgaatat cgcggcctat cgctattcga
    18061 cacagaatta ccttggtctt aatgatgcac taactctaat tgatgaagtg aaacatcccg
    18121 aacaagatct tgaaccgaaa tccatgcgta attactcgcg catgaaaaat caggttacgg
    18181 tcagtattaa ccaaccgcta aaatttgaga aaaaagatta cggttcattt tatctttccg
    18241 gaagttggtc cgattactgg gcttccggac aaaatcgtag caattactct attggctaca
    18301 gtaacagtgc atcctggggc agctacagtg tcagtgccca gcgttcatgg aatgaagacg
    18361 gcgacactga cgatagcgtt tatctcagtt tcaccattcc tattgaaaaa ttacttggca
    18421 ctgaacaacg gacttcaggt ttccagagta ttgatactca aatgagcagt gactttaagg
    18481 gtaataacca actcaacgtc agtagcagtg gctatagtga taacgctcgc ctcagttata
    18541 gcgtaaatac tggctatacg atgaataaag ccagcaaaga tttgagttat gttgggggtt
    18601 atgccagcta tgaatcaccg tggggaaccc tggcaggttc agtttctgca aatagcgata
    18661 acagtcgtca agtgtctctc agcaccgacg gtggttttgt attgcatagc ggcggcctga
    18721 ccttcagtaa tgacagtttt agcgactccg atacactggc ggtagttcag gctccaggtg
    18781 ctcaaggagc gcgaataaat tatggcaaca gtactatcga tcgatggggt tatggtgtca
    18841 ccagcgctct ttctccttat catgaaaacc gtatcgcgct ggatatcaac gatcttgaga
    18901 acgatgttga attaaaaagt accagtgcag tagctgtacc gcgtcagggt tcagtcgtct
    18961 ttgctgattt tgaaaccgtg caagggcaat cagccattat gaacatcaca cgaagtgatg
    19021 gtaaaaatat tccatttgct gcagatattt atgatgagca aggcaatgtc attggtaatg
    19081 ttggacaggg tggacaagca tttgttcgtg gcattgagca gcagggaaat atcagcatta
    19141 aatggctcga agaaagtaaa cccgtaagtt gtcttgcgca ttatcaacaa agttcagaag
    19201 cagaaaaaat agcgcaatct attattctga atggaattcg gtgtcagatt gagtaactac
    19261 aagggacgtc aaatgataaa aataacgcca cataaaatag cgatactgat gggaatatta
    19321 ttatcaccat cagtatttgc aacggatatt aatgtagagt ttacagccac tgtcaaagcg
    19381 acaacctgta acatcacact tactggtaat aacgtcacga atgatggcaa taataactac
    19441 acattgagaa tccctaagat gggtctggat aagatcgcga ataaaacgac agaatctcag
    19501 gctgatttta aactggttgc tagtgggtgc agcagtggca tcagttggat tgataccact
    19561 ctgaccggaa atgcatcatc aagctcacct aagcttatta taccgcagtc tggtgattca
    19621 tcttcgacga caagtaatat cggtatgggt ttcaaaaaac ggactactga tgatgccact
    19681 ttccttaaac ctaacagtgc ggaaaagata cgctggagca cagacgagat gcagcccgat
    19741 aagggtcttg aaatgaccgt tgcgctacgt gaaacagatg cagggcaagg cgtaccgggg
    19801 aattttcgtg cactggcaac gtttaatttc atctatcaat aagaaggata attaagatga
    19861 catttaaaaa tttacgttat ggattatcca gcagtgtcgt tttggctgcc tcattgttca
    19921 gcgtaccctc ttatgcggca acagacagca ttgggctgac cgtgattact accgtagaaa
    19981 taggtacttg taccgctaca ttagtaaatg actctgatca ggacatttct gttgttgact
    20041 ttggcgatgt atatatttct gaaatcaatg ccaagaccaa agttaaaaca ttcaaactca
    20101 aattcaaaga ctgtgcgggt atccccaata aaaaagcaca aataaaat
//
LOCUS       DAMZET010000074        19928 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.074, whole genome shotgun sequence.
ACCESSION   DAMZET010000074 DAMZET010000000
VERSION     DAMZET010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.074"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1406
                     /locus_tag="POB34_002692"
     CDS             <1..1406
                     /locus_tag="POB34_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDI5919603.1"
                     /translation="VNSRKGADKISASERKNHFGEIYCGLDPLQATYESDRCVYCAEK
                     ANCNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTL
                     KDHSGAVTIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARA
                     GVQVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFS
                     NLTSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTD
                     VEGKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEF
                     QFNVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFG
                     FQAHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAA
                     GRQAARDMLTLFDTKAS"
     gene            1406..1894
                     /locus_tag="POB34_002693"
     CDS             1406..1894
                     /locus_tag="POB34_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDI5919604.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(1930..3297)
                     /gene="ghxQ"
                     /locus_tag="POB34_002694"
                     /gene_synonym="ygfQ"
     CDS             complement(1930..3297)
                     /gene="ghxQ"
                     /locus_tag="POB34_002694"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HDI5919605.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(3333..4649)
                     /gene="guaD"
                     /locus_tag="POB34_002695"
     CDS             complement(3333..4649)
                     /gene="guaD"
                     /locus_tag="POB34_002695"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HDI5919606.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWENGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(4667..6067)
                     /gene="xanQ"
                     /locus_tag="POB34_002696"
     CDS             complement(4667..6067)
                     /gene="xanQ"
                     /locus_tag="POB34_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HDI5919607.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(6232..9102)
                     /locus_tag="POB34_002697"
     CDS             complement(6232..9102)
                     /locus_tag="POB34_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HDI5919608.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKRDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERIRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(9099..9878)
                     /locus_tag="POB34_002698"
     CDS             complement(9099..9878)
                     /locus_tag="POB34_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HDI5919609.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLASPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(9929..11257)
                     /gene="ssnA"
                     /locus_tag="POB34_002699"
     CDS             complement(9929..11257)
                     /gene="ssnA"
                     /locus_tag="POB34_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HDI5919610.1"
                     /translation="MLILKNVTAVQLHPAKVQEAVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFAAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDTLA"
     gene            complement(11260..14358)
                     /gene="ygfK"
                     /locus_tag="POB34_002700"
     CDS             complement(11260..14358)
                     /gene="ygfK"
                     /locus_tag="POB34_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HDI5919611.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVHGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVKKATIVYRRSVQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(14680..15258)
                     /gene="mocA"
                     /locus_tag="POB34_002701"
     CDS             complement(14680..15258)
                     /gene="mocA"
                     /locus_tag="POB34_002701"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HDI5919612.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIKRPNITNMRQALLMG
                     AHYSVEVENAEIILDIDTPDDFITAQKRYTKI"
     gene            15361..16131
                     /gene="yqeC"
                     /locus_tag="POB34_002702"
     CDS             15361..16131
                     /gene="yqeC"
                     /locus_tag="POB34_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HDI5919613.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRAMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            16179..17804
                     /locus_tag="POB34_002703"
     CDS             16179..17804
                     /locus_tag="POB34_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HDI5919614.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFI
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(17845..18777)
                     /gene="arcC"
                     /locus_tag="POB34_002704"
     CDS             complement(17845..18777)
                     /gene="arcC"
                     /locus_tag="POB34_002704"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDI5919615.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(18825..>19928)
                     /gene="hyuA"
                     /locus_tag="POB34_002705"
     CDS             complement(18825..>19928)
                     /gene="hyuA"
                     /locus_tag="POB34_002705"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HDI5919616.1"
                     /translation="GFGPNGCRLRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDE
                     IPMMVEEGLSSFKLYLTYQYKLNDDEVLQALRRLHESGALTTVHPENDAAIASKRAEF
                     IAAGLTAPRYHALSRPLECEAEAIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARA
                     NHQPVWVETCPQYLLLDERSYDTEDGMKFILSPPLRNIREQDKLWCGISDGAIDVVAT
                     DHCTFSMAQRLQISKGDFSRCPNGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMP
                     ARLFGLWPQKGLLAPGSDGDVVIIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRT
                     LSRGETIFCDGTFTGKAGRGRFLRRKPFVPPVL"
BASE COUNT         4816 a         5013 c         5220 g         4879 t
ORIGIN      
        1 ccgttaactc gcgtaaaggc gcagataaaa tttcagcaag tgaacggaaa aaccactttg
       61 gcgaaatcta ttgcgggctg gatccactgc aagcgactta tgagagtgac cgctgtgttt
      121 attgtgccga aaaagctaac tgcaactggc attgcccgct gcataacgct attccggatt
      181 acatccgtct ggtacaggag ggaaagatta ttgaagcggc agaactttgc caccagacca
      241 gttccttacc agaaatctgc ggcagggtat gtccacagga ccgtctttgt gaaggcgcat
      301 gtactttgaa agatcactct ggcgcagtca ctatcggtaa tctggaacgc tacatcaccg
      361 ataccgcgct ggcgatgggc tggcgtcccg atgtcagcaa agttgttccc cgtagcgaaa
      421 aagtggcggt gattggcgct gggccagcag ggttagggtg tgctgatatt ctggcgcgcg
      481 caggagttca ggttgatgtc tttgatcgcc atccagaaat tggcggtatg ctgacttttg
      541 gcattcctcc tttcaaactc gataaaacgg tattaagcca gcggcgagag atattcaccg
      601 caatgggaat cgactttcat ctcaactgtg aaattggccg cgatattacc tttagcaatt
      661 taacttctga atatgatgca gttttcatcg gcgtggggac ttacgggatg atgcgagcag
      721 atctgccgca tgaagatgcg cccggtgtca ttcaggctct accgttcctg actgcccata
      781 cccgccagct catgggattg ccggagtctg aagagtatcc gctgacggac gtggaaggta
      841 agcgagtcgt ggtattgggc ggtggcgata cgacaatgga ttgtttgcgg acttccatcc
      901 gcctcaatgc cgccagcgtg acctgcgcgt atcgtcgtga tgaagtcagt atgccgggct
      961 cgcgcaaaga ggtggttaat gcgcgcgagg aaggtgtcga gtttcagttc aatgttcaac
     1021 cgcaatatat cgcttgtgac gaagatgggc gtttaactgc ggtgggcctg attcgtaccg
     1081 ccatgggtga gccggggccg gatggtcgcc gtcgtcctcg tccggtagcg ggttcagagt
     1141 ttgaattgcc cgccgatgtt ctgattatgg cctttggttt ccaggcgcat gccatgccgt
     1201 ggttgcaggg cagcggaatt aaactcgata aatggggcct gattcaaacc ggtgacgtcg
     1261 ggtatttacc tacccagacg catctgaaaa aagtctttgc tggtggtgat gcagttcatg
     1321 gcgcggatct ggttgtcact gcaatggccg caggaaggca ggcggcgcgc gatatgttaa
     1381 ctctgtttga tacgaaggca tcgtgatgaa atcgttaatt atcgttaatc cggctgactg
     1441 tattggctgc cgtacttgtg aagtagcctg tgtggtcgct cacccttcag aacaggagtt
     1501 gaatgccgat gtctttttac cccggctgaa ggtacagcga ctggacagca tcagtgcgcc
     1561 ggtgatgtgc catcagtgtg aaaacgcccc ttgtgttggc gcttgccccg tgggggcgct
     1621 gacgatgggc gagcaggtgg tgcagaccaa ttctgcccgt tgtattggct gtcagagttg
     1681 cgtcagtgcg tgcccgtttg gaatgatcac cattcagtcg ttgccggggg atacccgaca
     1741 acaaattgtg aaatgcgatc tctgtgaaca gcgggaagag gggccagcct gtgttgaatc
     1801 ttgcccgaca caggcgctgc agttgcttac tgaaagagaa ctcaggcgag tccgccagca
     1861 gcgtattgtt gccagcggtg agaatcctct gtgaaagaaa tctccgcgct gaaaggcgcg
     1921 gagaatctat tagattgccc aaccacccgc gtaaaatgcg accagtgcgg cagtaatgat
     1981 caccgtacca atattcagct tttgccattc gcgtgcaaag acgcggccta cgaccagtgt
     2041 cacaaagccc agcataatac cggtaacgat attacaagtc agaacgatga acacggcaca
     2101 caccaggcca gccatagcgt caataaaatc attgaaatcc agcttcgaga cgttacttaa
     2161 catcagcaaa cccacgtaca tcagagcggg tgcagtggcg taaccaggga tcaaaaatga
     2221 cagcggtgat aagaacagaa tcagcaggaa taacgccccc actacggttg cggtcaaccc
     2281 tgttttaccc ccggcggcgg ttcctgccgc tgattcgata taaaccgctg cgggcgctgc
     2341 gcccaccaga ccggagaata ttgaacttac tgagtcactg gtcagggctt tgccgccgtt
     2401 gatgatctgg ttgtctttat ccaacaaatt cgcctgaccg gcgacggcac ggatggtgcc
     2461 agtggcgtcg aacactgcgg tcatcaccaa tgccagtaca ctcggaagta cagttggctg
     2521 gagtgcgccc ataatatcga ggctgaaaat cagagactta ccatcttcgc cagtcaggct
     2581 tggcatcgcc accagaccgt ggtatttcac cgctgggtca aagattaagc cgatgatcga
     2641 aattgcaata atcaccaaca agatcccgcc gggtacgcga cacttctcca gaccgaagat
     2701 gaccgccagc cccagtaagc tcatcatcac cgggaaggag gtaaacgcac cgagcgccac
     2761 tggcaagcct tcaatcgggt ttttgataac cattcccaca ccgttagcag caatcagcag
     2821 cagaaacagg ccgataccga tacctgtacc gtgagcgatc cccatcggta aattacgtaa
     2881 gatccaggta cgcacaccgg ttacggaaat agcggtgaag atgacgccca tcagaaagac
     2941 cgcgcccagt gcgacaggaa cgctaatttg ttgcccgagt accagactga atgcggtaaa
     3001 cgccgtcaag gaaatcgcgc aaccaatcgc cattggcaaa ttggcccata atcccatcag
     3061 caacgagccg aagcccgcga ccagacaggt ggcaacaaac acagctgcgg gaggaaaacc
     3121 tgctttgccc agcattcccg gaacgacgat aacggaataa accatggcca gaaaggtcgt
     3181 taagccagcc agtacttcct gacgaacggt actgccacga gcggtaattt taaaataatt
     3241 atccagcgcg ccctgtggct ttggtgcgtc cggtgtttgt aggatgtctc cagacataat
     3301 gatgtcctca cgaggatgtt aaagttttat tattagttgc gttcgtacac cagacgacca
     3361 tcaacgtagg tgcggtagat cgaacggtca tcgcccaacg tcatcatcac gaacaatttg
     3421 tcgactaaag aaacagagtt gtcatagcgc agctgctgta gcggagtggc ggtgggttcc
     3481 atcaccacga aatcagcctc tttgccaggt aaaaagttgc caatcaaatc gtcaaggccc
     3541 agagatttcg ctccgccgag cgtggccagg taaaacgctt catatgccga gaggcgatag
     3601 ccttgtaatt gcaacacttt gtaggcttcg ttcagcgttt gcagcatgtt gaaagtggtt
     3661 ccggcaccga tatccgttcc catgcccact ttaactttct tctgccatgc ttttttcaag
     3721 ttgaataagc cgctgccgag gtaaaggttg gaggtcggac agaaagcaat gctggatttg
     3781 gtttcgctga gacgatccca ctctttttct tcgagatgga cgcagtgagc aaagacacag
     3841 tttttaccgg tcaggccgta ctgatggtaa acatccagat aaccatcatg gtcaggataa
     3901 agcgatttca cccaggcaat ttcatctttg ttttcacaga gatgggtatg tacccacgta
     3961 tccggatatt cttctttcag gcgttgcgcc atcgccatct gttcaggaga tgaggtcggg
     4021 gcgaagcgtg gcgtaatcgc atatagcaga cgaccatttt tgtgccagcg ttcaatcagt
     4081 tctttgcttt ggtgatagct gctttcggca gtgtcgagca gataatccgg tgcgttacgg
     4141 tccatcatca ccttaccggc aatcatacgc atattgatat gactggcggc ttcaaacagc
     4201 gcatcaacgg attgcggatg aacagtgcca aacaccagcg ccgtggtggt tccgttacgt
     4261 aaaagctgct tgatgaagaa cgccgacatt tcgcgggcgt actctaaatc ctcataacgc
     4321 cgttcagtag ggaaggtgtg tttattcaac cactccagca attgctcacc ataggccccc
     4381 accatttcac tttgcggata atggatatgt gtatcgacaa agcccggtac tatcagtttg
     4441 ccgcgatagt cgcgcacgcg aatggtgtca ggaatttgat gctttccgtt ttcccattcg
     4501 ccaaaccatt ccacttttcc ctgtttaatg agtaataaac catcctcaat aaaccgcagc
     4561 gcagaggcaa tctcttccgg gttatcgacc gtacgggtga catcaataaa actgcctcgt
     4621 accgctttta acgtgtgttc tcctgacatc attgactcct ttactgttaa tccatctctt
     4681 ccgctgaggt aataccaggc agaacgtttt cctgtcggta gccaccaggg aggataatat
     4741 ttaataaaat cgcagttaac ccgccagcac aaatagggtt ttcaactaat acataaatag
     4801 aggctggcaa tattttaaaa atttcgggat cgtaggagac gccaagccct aaacctaaag
     4861 aagtggcgac aataagtgtt tcacggcgct ttaaaccgtt ggtgatgatg atgcgaatcc
     4921 ctgcgatggc aatcatggaa aacatcaacg tcattgcgcc tcccagaact gccgagggaa
     4981 tggtcgtgaa gaagccgcca atcatcggaa ataagccgag gataaccagc attaccgcga
     5041 tggttcgccc gacataacgt gaagcgacgc cagtcatctg aataacccca ttattttgcg
     5101 caaacgtggt taatggtaat gaaccgacag cggaggcgat aacagaaacc agaccatctg
     5161 ccagcacgcc gcctttcagt cgggactgat actcttcccc ctgaatgggg cggcgggaaa
     5221 ccattgccgt ggcggtgata tcgccgactg cttccagcac gctaagcaga taaatcgtgc
     5281 caaccaccag gaactgatgg aagctaaaac taaagccgta tttgaatgga tgtgggatgg
     5341 tgattaacgg caaattgcgc atactgctga aatccaccat gcccaggcat aacgatgcga
     5401 tatagccgac acatagccca atagcgatcc ctcccatgcg tagcaacgga ctgcgacagc
     5461 agttaaagcc gatcaccaca atcaaaacca ataaaccaac gccgagatgt tcgtaattgc
     5521 cgaaggtacc gctgcttttg gcagcaaatc ctccaccaaa atcaataatg ccgactttaa
     5581 tcaggcttaa gccgatcatc agtaccacaa taccgctgac agtaggcgta ataacccgac
     5641 gtaaataggg caggataaaa gaagagccga caaccagaaa tgcgccaacg aaggagacgc
     5701 cgagaagcga cgacatgatt aactcttcgt gaaaaccgtc gcttttcatg ctgctgccca
     5761 gcgcaatcat gaccgtaaca aatgaaaaat tgactgactg aattgagagt aggccagaac
     5821 cgacgatgcc gtagcggttt acttgtaacc aggtaccaat accagaggcg atcatcgcca
     5881 ttgaaacaag ataggcagtt gtttcagcgg aaagctgtaa ggccgcaccc acgattaacg
     5941 cgggggttac catcggaacg aaaattgcca acagatgggt aatggcacca acgagagcct
     6001 gatgaaaggg agggcgatcc tccagttcaa atataaggtc agaacctgca tggtttatat
     6061 cagacatccc ttcactcctt ttaaagtaaa tttgatcatc tgaattagta aaataaattc
     6121 ccacgaattt ggggcgcagg acgccttgag aacggcttat ctcattttcg cagtcactat
     6181 ttagtcatcg ttcaaacaga gaaacaggcc gccgaagcgg ccatttatta cttatatttt
     6241 ttccagcgcg gtgagtattt tctccggtgt gaaatgccat tcgcgtaacc agatgccgca
     6301 ggcatcgtga attgcggtag caatcgccgg agctgcgcca tttacaccga tttccgagat
     6361 cgatttcgcc ccgaacgggc cgactttatc gtcgctcggc accagcacag cgcggaaatc
     6421 acgcggaatg tcaccaattt tcggtgcgcc gtaactgcgt aaatcacgcg ttaacgggtg
     6481 accttcggcg tcataaatga tctcttcgct catactgtgg ccgatagctc gcagggtagc
     6541 accgtagatt tgtcccaatg ctaattccgg gttgaccggt gtaccgcagt ccagcaaggc
     6601 gtagaattta tccaggcgga tttcacccgt acgcgtgttg acggcaactt cagcgaagtt
     6661 tgcgccatac gggaaggcga aatcaggcgt gatataactg ccagttccca ccagtgaacc
     6721 aaagccggtg ccggtttcgc ctttatgagc aatatccccg aagctgactt cgcctttctt
     6781 gccgcgcacg acgcccggcg ttgccagttg aacatctgcc actgcctcac ccaacatttg
     6841 cgcgccgtgg aacagaattt tctcccgcag attttccgct gccaaacgcg ccgcgttacc
     6901 tgagaagcaa gtaccggacg aggcatatgc gcctttatca aacaacgcat gatcggtatc
     6961 accggagata acatgcacgt cctgcggtgg gcaatgcagc acttctgctg ccagtttcgt
     7021 caccacagta tccagaccag taccaatatc cgcaccgcca gaatgaacga taaaggtgcc
     7081 gtccgattcc agtttgatca tgcagttagc ctgatcgata tccgggatac ccgatttctg
     7141 catgataatg gcgacgccgc gaccgatgtg ccagtcacca ttttgcggtt tcggtgacga
     7201 ccattggatc atctcgcgac cctgacgcag gatctcttct agtgcacagc tggcggcgga
     7261 aggaacggag gtcggcgctt taccttcacc gattgcaccg agaattttca gctcttgccc
     7321 ttcgtgtacc cggatacgtt cgataatttc cagttggtcg atctgtaact gttcagccag
     7381 ttccgctaat gccatggtga tagcgaagtt acctttcggt gcgccataac cctgataagc
     7441 accatttggg caaatgttgc tgtagtaggt ggtgacctga aaatcgacgt tatcgcacgg
     7501 atataacggc agcgacagcg ccggtccgtt acatggtacg gtgagtgagt ggttgccgta
     7561 agggccagtg ttggcgcgga aatccatctt cactgctgtc aggcgaccat ctcttttcgc
     7621 tcccagtttg acggtgactt tcgcgacgtg acgagaggtg ttagcaataa actcttcttc
     7681 acgggtgtag cggaacagta ccggacgccc ggtcacgcag gttgcccagg cgcacacttc
     7741 ttccagcagg atgtcctgtt tggaaccaaa accgccgccg actcgctctt taatgacatg
     7801 aactttatgc tgtttcatgc ccacgaggcg cgcgacctgg cggcgtaagt gccatggtac
     7861 ctgggtggag gcgtggataa ccagacgatc gccgtccata cgagtaaagc agatatgtgt
     7921 ttcagtcggg cactgctgcg cttgcgttga gttataggtt cgctcaatga tcacatcggc
     7981 atcggcaaag cctttgtcca tatcgccaat atgaccatga atactggcgg cgatattttt
     8041 gcgggggcga gaaccgatcg ggaagttaat gatcatatgc tcgccgcgct gggctgcatg
     8101 gctgttatcg tcttccagag tatctggcgc accagcaaca tacaccaccg gttcatcgtg
     8161 cacgacaggc gcatcttccg ccattgcttc gtcgatcgac attaccggct taagcacttc
     8221 atattcaacg tcgatgagct tcagtgcttc gagcgcaatt tcttcacttt ctgcgaccac
     8281 cgcagcaaca cgatcgccga cgtgacgcat tttcttgccg aacatacggc ggtcaagcgg
     8341 tgacggttcc ggtgcgctct gaccccccgg ggtatagtag atatccgggc aattcaggtg
     8401 agtaataacg tgaacgacgc ccggtaaggc ttcagctttg ctgacatcca gatgagtaat
     8461 cagtgcgtga gcgtgtgggc tacgtaacat tttaatgacg caggcgtcag ccgttacgcg
     8521 gtcttcaaca tagcagggtt tcgcctgcac cattttcgcg gcatcagttt taggataatg
     8581 cttgccaatg acttcaaggt cgtcacggaa agtcggagcg atatcaatgg tggcctgcgg
     8641 attattttta cgtgcaaccg ccagttcaat gacctgatag tattgttgcc agcctgcatc
     8701 gcggctgaac aaaccagaaa gcgcgtcgtc gatctcttcg cgggtaggtg cggcgatgcg
     8761 atcgagaaga tccgtgataa tcagagctgc agctggatcg ttataaccag actgcaccac
     8821 gccaacatca accattgcct gttgaaccag acttaactcg ttccatttgc ccagagattc
     8881 tgcggtacga atatctgcct tctctaactg tgcggcaata agcaaggacg cgttaacgat
     8941 attaccgtta aagattattg cgtcagaacc ggcaaacccg aagccatcat cactgttgcg
     9001 tacagagtgc attcccatgt taaacaacag cttttgcacg ttttcgcctg gattaacggt
     9061 tagctcctga ggcgcgccat ttaaagtaaa gtggatgatc atacggcttc ctccccagcc
     9121 tgttggcagt cggcatacag gtcggctacc agaactcccg tgatatagcg tttataggcc
     9181 acgctgcccc gcaaatcttc ctgcgggaaa atggcgttgg cgacagcctg ttcaagtgca
     9241 ttgccttcca gattttgttt ttcgacatcg tgcagacgca gtgctttact ggcgacgcca
     9301 tccagcgcaa ttcgcatacc gtcgtggtct gtcattgcaa cggctgccgt cacgacggtt
     9361 aaacctgcct gagaacggct aattttgcgg gtcgcacagg tgcgatacgg atctttaatg
     9421 ataatttcgg ttagcaggcg atcgcatggg ctggccaggt agtcctcgat tgacagcgtt
     9481 tcgccgttgc caaaaaccag ttcagcatcc agtgccagca ggacaggaag cagcaccgac
     9541 tcttcctgcc gggcggcgat ttcaccacca atggtcgact gattacgaac atggcgtgag
     9601 taaacaaaac cgagggcttc acgcagtgct gcaggaataa atcgcgcatc acgcagtggc
     9661 tgcaagcgag acattgcacc aatccgcagt gcaccgttat cccagtcaac ccagtccagt
     9721 tccagatcct gtaaggaaat ggcaatcttt ttatcggtac gggttggtgt agcgttgagt
     9781 ttgctgcccc cggcgaacca gacggcttca tcctggtagc ggcgcttcag ttccagcgcc
     9841 tgttcgacag agtcgggcct gaaaaattgt tcaatcatct tctttcctta tttaaccctc
     9901 ttccctgcgg gaagagggca tctgtcattt atgccagcgt atccatccga cgccacatac
     9961 tggcagcggc ttttctggct tgtgcataaa tggaatcgca atcaaagtta aactgacggt
    10021 cttcatagac catcacgcca ttgaccatca cgctgtgaac gctgcctgag cccataccga
    10081 aagcgatatg cccggcgata ttgtctgcca gcagcggcgt cggcgagttg taatcgcaaa
    10141 tggttaaatc ggctttgtaa ccggcttcca gaagaccaaa tttcgcggca aagttgcggc
    10201 tcatcagttc gttaccgtta gtcagggctt tggcaaaact gtcaggccac agcggaccac
    10261 ccgcatcgcg atgtttaaag aaggcaaatt tcatctcttc aaacatgtcc gaaccaatgc
    10321 cgtccgttcc caacgccaga ttgcggatgt cgctaaggtg atggttgtag ccgacatggt
    10381 tgttcatgtt tgaacgggcg ttatgcacca ggaacgcatc gcgctgattg agtagggcga
    10441 tgtcatcttt cgacaagtac agcccatgag cgaccagcgt tttgctgtcg atgagatcga
    10501 attgcgccag tcgtgccagc aggtctttgc cgtaccagtg gtgactgtag gaaacgtcgt
    10561 aaaggtcttc cgcagcgtga atatgcaaac cacgacctgt ggctttcacg gcttcacgca
    10621 gcatctccag accggcatct ggcacggtaa acggcgcgtg agcaccgata tgtgcttcca
    10681 ccagatacgg ctcggttgct gctttcttcg cctcatcaat ctgacgggca aagcggatgt
    10741 tttcttctac accttcctgc aactctttga tgccgttgtt acggtcagta gtttcaaaac
    10801 aggtcattgc gcgcaggcca acttttaaaa atgcgtcgcg caatgtggag agcgacccgc
    10861 cgatatacgc tggagaggca tggtgatcga taaccgatgt acatccgctc ttaatcgctt
    10921 ccagggaaca aatcagcccg ctgtaataga gcgactcttc atcaagggcg cgatcgagcc
    10981 gccaccagag atttttcagc gttgagatga aatccgggca gggggcgatg tttgccataa
    11041 ttccgcggga aagccccgag taaaaatggt tgtgcgagca gacaattccc ggcatcacaa
    11101 tccgaccatg catctctttg tagctggcat cggggtagcg ttgcgtcagg gcatcgccga
    11161 tagcgacaat cacatcattt tcgatagcga tatcaacggc ttcctgcact ttcgccgggt
    11221 gtaactgcac tgcggtgaca ttcttcagaa tcaacatgat tacacctcca cgcggcccag
    11281 cagataatga tgatgctggt ggacatggct gatgatgcgg cacatatcgt tcagctccgg
    11341 tggtacgttg ttgaactgac cttcgctgtc gatgtttaac acccagcttt ggttattcag
    11401 acgtactcgt acccggcaat cttccacaag gaagcctggg ttgctgctgt tatcaaagtc
    11461 ttgcgacagg ctgaagacgg tgattttgtc tttgtatggt ttaccgttcc acggacagaa
    11521 ctgagcgcag ttgccgcatt cgttacagta agcgtcgagg tgcagcgtct ggaaacggtt
    11581 ctggaagcct gggaccgcaa tggagacgtt ggcgcggttc gggcagacat ccacgcactt
    11641 gctgcaaacg tagttacatt caaggcagcg agcagcttcc tgcgcgacaa acgcatcacg
    11701 atcgtcactg ttcaccagcg tgatagagat atcgccttta cgttgataga tttccgccgg
    11761 attgacgttg ttccagtatt tatcgttctg gtgggaacgg atattttccc gactaaggat
    11821 ggcatcggtc gcccgacgcg cggttccgac agcagcgaca atggaggatg gtccgcgctg
    11881 tacgtcgccg atcataaaga cgtcagtcag acgagtttcg ccattatggt cgacgtctgg
    11941 ccagccgttt ttgtccagcg gcacgcccat cgcattcagg gcttcagtat cctgctgttc
    12001 accaatggcg gtgatcaggc tgtctacatg cagtgttact gtttcgttgg tttcaaccgg
    12061 acgacgacga cctttctcat ccggttcgcc aagcgacata acgcgcaagg ttaaggtgcc
    12121 atcagcatcg aaacgttccg gattattcag gaaacggaac tccacgccgt cgtgcaacgc
    12181 ttcttcatac tcttcgcgcc atgcgggcat ctcttgcact gaacgacggt aaacgatcgt
    12241 tgcttttttt acgcctggaa cgcgtaacgc cgcacgagcg cagtccattg cggtgttacc
    12301 cgccccgaca acgaccacat gtttgcccag cttgagcgct gtacccttgt tgtattcacg
    12361 caggaagggg agtgatttcc agacattttg gttgtcgccc gccagtttca caccgctatt
    12421 tttatcagtg ccggtggcaa tcagaacata gtggaagccc tgatttttta actgttcaac
    12481 ggtcaaatcg ggtgagcagc catactcaaa tttcacgccg tgagcggcaa caaaatcgat
    12541 atcgtgctga attaactctg caggaatacg gaactgaggg atgatatttt tcaccacgcc
    12601 gcccgcattg gcttcgcgtt caaacagcgt aaccggatgg cccgctctgg caaggaagta
    12661 acctgcagcc agacccgccg gacctgcacc aatcacggca accggatggc gtgaaccaga
    12721 accggctggt ttgtgccagc gttgcttata ttcatcccaa cctttttcca gcgcgacttt
    12781 tttcagttcg cggatattca gtgcactgtc gtaatccagg cgggtacagt tgtattggca
    12841 ctggtgatcg caaatatgac cggtaatggc gggcagggcg ttgcgttggt agataagttc
    12901 cagcgcgtcg gcatagcggt gttcgccaag cagacggatg tattccggaa tatcttgctt
    12961 gatcgcgcag gcagtaacac agggggcaac gtagcagtcg gtcagcggca ggtcttctgc
    13021 cacttcaata cgctcttctg gcttccagtg tttctgggtg tattccatgg ttagcgcgtc
    13081 tgctgccagt ctgttcagtc gttcgacgtc aacatggtca agtccccagg cgtcggagcc
    13141 ttccagctcg cgcatgcagg cacttaaacg cagatagcca ccaggtttca gcaggtcggt
    13201 tgccatagta atagggcgaa tacctgtatc aaaaatatcg cggatagtca gctgactggc
    13261 accaccggaa taagaaatgg gcagtttgcc gtcaaaggcg cgagagagaa ctgctgcaac
    13321 attgatggag agcgggaaca gcgcacggcc tgacatatac atctcttcac caggcagtgc
    13381 gcctttattg ttgatggtgc cgagagtgtt agtcagtttt acgccaaagc cgagtgattt
    13441 ttcttttgcc agtgccatca ggcgttccag catttccagt gcttgcgtca gcttgaggtc
    13501 gtgatcaaat gactcttctt ttaagcctat gtaaccgaaa ccgcagacat cgaggatctc
    13561 acgaacacgc gcgtacccca gtaaggtcgg gttaagtttc acaaaggtgt tgagcccttt
    13621 ttcttccagc atgtagcggc aaatggcttc gatttcatgc ggaggacagc cgtgcatggt
    13681 ggagagggtg acgccatgca ccatactggt ggggatgcga gcgggtaagg cttgcaagct
    13741 ttcgcgtttt tcctgcaatc cgtggcgggc taaaaatgcg tcatcctgga gtaatttatt
    13801 cagcgtatcg cgatattgcg cgaatttcgg atggtcagat gcgtccatca tattgtcgat
    13861 gaactgttgc attggcggtt gcttaatacc ttcgaggttg taaccgacgc tcatattaaa
    13921 gatgaacgat ttaccggaat cagaaggttg gaacatcgct tcgagaaggt gcagggcaaa
    13981 ccaggctttg aggtattcat cccaggcttt aagcagggta aattcggtag accattcggt
    14041 gttaaagcac tcgtcttcgg catcaataca gggcttttcc agctccaggc ggtcaagaat
    14101 ttggacggtt tttagttcga tgaaacgtcc gccagtcagc caggaagtga caatattttg
    14161 cgcgagctgc gtgtgcgggc cagcggcagg gccgacggga gtggcacagg tttcaccgaa
    14221 gacgctaaca gttttacctt ttacaggtga gtaaaactgt tgctcgggaa taccaaagat
    14281 tgagcgttgt tgttggtatt catcaaatat gcgcgtcaaa agttcctcaa acggaatggg
    14341 acgcataata tcccccataa ccctctcctt agcttataca gatatcattg agtgaccggt
    14401 gttgagaaat ccggtggttt agggggatag agcaacaatc gcaccactct tgccaggttt
    14461 ggcgggaaat ataaaaaaat gtgaggcggc tcgaaactta agggcttttt gtgaatgaac
    14521 tcacaggtgc taaatgagaa ttaaaatcac tcctgaaaat tattattgcc agcccatata
    14581 tcactttttc tcttttctca aaatcagtga ttccttatca ttatgataaa gagtgctgga
    14641 gggattaaat atttgaacgc aatcgtttaa ctacgttttt taaattttag tatacctttt
    14701 ttgcgctgta ataaagtcgt ccggagtatc aatatctaaa attatttctg cattttctac
    14761 ttcgacggaa taatgagctc ccataaggag tgcctgacgc atattggtga tattggggcg
    14821 cttgattgcc tgcatcaggc atggttttga cactaaaatc ggatggccgg ggatgccatt
    14881 atggagtggc agtattgcgc catcatttcg taacgaccag atttttctga aaatatcgat
    14941 ggtgagggtt ggcatgtcac cgtgggtgag aaaacaatgt tctgtttgca ctgcgggtac
    15001 tgcggccttt actgatgtta gtaaaccctg cgcataatct gggttgtgga taatagtgat
    15061 attgctctgg ttcgcataac gttcgtgcag ttcattacca cgatagccgg tgactaaaat
    15121 aattcggcta caaaactgca acgcattttt gatacttgta tcaagaattg tcccctgttg
    15181 ccagggtaac atcattttcc attgtcccat tcttgatgat aatccggcag cggtaattat
    15241 acagtcgatg gctgacatag tggttccatt atttataggg gctaataacg cctgataatt
    15301 gcgttacttc ctgatggatt aagttgggga taatttatcc cagagaggtc ataaagactc
    15361 gtgaaaagta tagttgaccc atcggcatta ttcattgatt taggtgcgca gaaacgtccc
    15421 actgtgattt ctgttgttgg tgctgggggg aaaaccagct tgcttttttg gctggcagaa
    15481 ctgctccagg ctagcggcag gcgtgtgtta atcacgacga caacacatat gtttatgcca
    15541 acgtctcact ggcccgtggt tttctgtcgt gatcccgcca tgcttcctca tgcgtctctt
    15601 acatccccca tttcattttg ttttcacagc tggaaagcga accagggaaa agtgcaggga
    15661 tttacgccag aagcgattga tgcactggtg caacgaccag agtgtgacgt aattctcatt
    15721 gaagcagatg gctcgcgtgc aatgccgtta aaagcgcctg atgagcacga accttgcata
    15781 cccaaaagca gttgctgcgt gattgctgtg atgggaggac atattttggg cgcgaaagtg
    15841 agcacagaaa atgtccatcg ctggtcgcag tttgctgata ttaccgggtt aacacctgat
    15901 gcaaccttgc aactgagcga tctcgttgcg ctggttcgcc accctcaggg ggcgtttaaa
    15961 aacgtaccgc aaggttgtcg gcgagtctgg ttcattaacc gtttttctca atgtgagaat
    16021 gcgattgcgc aaagcgagct ccttcaaccg ctgcaacaac acgacgtaga ggcaatctgg
    16081 ctgggcgata tacaagaaca tcctgcaatc gcgcgcagat ttgtgaatta gcggcccggg
    16141 tattccgggt tactgaaagg atatccattg aggttggtat gaatattttc acagaggctg
    16201 caaaactcga agagcaaaat tgtccgtttg cgatggcgca aattgttgat agccgaggct
    16261 cgactccccg ccattctgca caaatgttag tgcgcgccga tggttctatc gtcggtacaa
    16321 ttggtggcgg aatggttgag cggaaggtga ttgaagagtc acttcaggca ttgcaggagc
    16381 gtaagccgcg attattccat ggacgtatgg ctcgtaacgg tgcggatgct gtcgggtcag
    16441 attgtggagg tgcaatgtca gtatttatca gcgtccatgg tatgcgtcca cgtctggtgt
    16501 tgattggcgc ggggcatgtc aaccgggcga tagcccagag tgcggcgcta ttaggatttg
    16561 atattgccgt tgccgatatt tatcgcgaaa gcctcaatcc tgaactattc ccaccatcaa
    16621 ccacgcttct ccatgctgag tcgtttggtg cggcagtgga agcactggat attcgccctg
    16681 ataattttgt cctgattgcc acgaataatc aggatcgtga agccctcgac aaactcattg
    16741 aacagcccat tgcatggttg gggttgctgg caagtcgtcg caaggttcag cttttcctgc
    16801 gtcaattgcg tgagaaaggc gtggctgaag aacatattgc ccgtttacat gcgcccgttg
    16861 gttacaacat aggtgcggaa acgccgcagg agatcgccat cagcgtgctg gcagaaatat
    16921 tacaggtgaa aaataacgcg ccgggtgggc tgatgatgaa accttctcat ccttccggac
    16981 accagctggt ggtgattcgc ggtgcgggag atatcgccag tggtgtggcg ctacgtctgt
    17041 atcatgcggg ttttaaagtg atcatgctgg aagtggaaaa accgacagtg attcgttgta
    17101 ccgtggcgtt tgcccaggcc gtgttcgatg gcgaaatgac ggtcgaaggc gtcactgctc
    17161 gcctggcaac cagctctgct gaagcgatga aacttaccga acgcggattc atccctgtga
    17221 tggtagatcc cgcctgttca ttgcttgatg aactgaaacc gctttgcgtg gtggacgcta
    17281 ttctggcgaa acagaatttg gggacgcggg cagatatggc accagtaaca atcgcgcttg
    17341 ggccgggctt tattgcaggg aaggattgtc atgcggtaat tgaaacaaat cgcgggcact
    17401 ggctcggtca ggtgatttac tctggttgtg cgcaggagaa taccggtgtt cctggcaata
    17461 ttatggggca taccacccga cgggtgatcc gtgctcctgc tgcaggcatt atgcgatcca
    17521 acgtgaaatt aggcgatctg gtgaaagagg gcgatgtgat tgcctggatt ggtgagcatg
    17581 aaattaaagc accgttgacg gggatggtgc gtggcttgtt gaacgacggc ctggcagtgg
    17641 ttggtggttt taaaattggt gatatcgatc ctcgtggtga aaccgctgat ttcaccagcg
    17701 tttctgataa agcccgggcg attggcggcg gcgtacttga ggcgttaatg atgttgatgc
    17761 atcagggcgt gaaagcgaca aaagaagtgc tggaagtggc ttaaatgaaa atggccggat
    17821 ggtattaccc acccggcctt aaaattactg acaaatcacc gttccggttt tgccttcaat
    17881 cccttctttc gctttgctca atacggtaat cagtgcttca cgacctgcgc gagagcgagc
    17941 aaacgaggcg gcagcttcca ctttaggcag catagaacct ttcgcaaaat gaccttcttc
    18001 gataaagcgt tcagcatcac ttaatgacag gcgatcgagc cactgttcat tctctttacc
    18061 gaagttaatg gccacttttt ctaccgcagt cagaatgatc agcatatcgg catcaatcat
    18121 ttctgctaaa cgggcgctgg cccagtcttt atcgataacc gcgctggcac cacgcagatg
    18181 gttaccttca cgaataacag ggataccacc accgccaacg gtgatcacca cctggcctgc
    18241 atctaccaga gctttaaccg tttctttttc aatgatatca accggttttg gcgaggcaac
    18301 aacacggcga tagccgcgac cagcatcttc tttcagagtg taaccctgtt ttgtcagttg
    18361 ctctgcttct tgctcggtaa agaacgaacc gatcggcttg gtcgggttga ggaatgccgg
    18421 atcgttagcg tcgacttcga cctgagtaac cagcgttgct acaggtttat taatgccccg
    18481 agaaagcagt tcttctctta acgcgttttg tagatcgtaa ccaatataac cctggcttaa
    18541 ggcaacacag acagacatcg gcagcattgg cgagtgtgct tcagtttttg cggcagcttc
    18601 aaaggcctgg ttaatcatgc ccacctgtgg cccgttaccg tgagtgacga tgacctcatg
    18661 tccctgggca attaaatcaa caattgcctg agaagtaatt tttaccgctt tcatttgccc
    18721 ggccagatca tcgcccagcg cattcccgcc cagggcgaga acaattttct tactcatttt
    18781 tcctcactca tcattcttga ttgtgtatag atccctggca gtggttagag cacgggaggg
    18841 acaaacggtt tgcgtcgcag gaaacgacct cgcccggctt tgcctgtaaa ggtgccgtca
    18901 cagaaaatcg tttcaccacg ggataaggtt ctgacaatcg cgccctgaca ggtaaaaccc
    18961 tcccatggcg agtagtcggc gttgtcgtgg agatggcgat gctgaatttg ttggctctga
    19021 cgtgggtcga taatcaccac gtcgccatcg gaaccgggcg ctaataatcc tttttgcggc
    19081 cacaggccaa acagcctggc gggcattgcg ctggttaatt caacaaagcg ttccggtgtt
    19141 atacgtcccg tcatcacgcc actggaaaac agtaactgca tgcggttttc cacgccgggt
    19201 aagccatttg ggcagcgact gaaatcgcct ttagaaattt gcaggcgttg agccattgag
    19261 aacgtgcagt gatcggtcgc caccacgtca atcgcaccat cgctgatgcc acaccacagt
    19321 ttgtcctgct cgcgtatgtt acgcagcggt gggctaagaa tgaacttcat gccatcttct
    19381 gtatcgtaac tgcgttcgtc caacaggaga tattgtgggc aggtttcaac ccagactggc
    19441 tggtgattcg cacgggcaag acgcagataa tccagaccta agccgttaga caggtgcacg
    19501 atatagagcg gggcgttacc ggcaatttgt gccaggttaa tcatgcgggc gatggcttcc
    19561 gcttcgcatt ccagagggcg actcaaggcg tgatagcgcg gcgcggttaa ccctgcggcg
    19621 ataaactccg cccgcttgct ggcgatagcc gcatcatttt ccgggtgcac ggtggtcagc
    19681 gcgccggatt catgcagacg gcgtaatgcc tgcaaaacct cgtcatcgtt gagtttgtat
    19741 tgataggtta aatagagttt aaaactgctc agcccttcct cgaccatcat agggatttcg
    19801 tcgaggatgg cgtgattaat gtgctggatc acaccatgaa agctgtagtc gatgaccgcc
    19861 ttatgggcgg catagccacg ataaacctcc agttgatggc gtaaccgaca cccgtttggg
    19921 ccaaatcc
//
LOCUS       DAMZET010000075        19807 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.075, whole genome shotgun sequence.
ACCESSION   DAMZET010000075 DAMZET010000000
VERSION     DAMZET010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.075"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1195)
                     /gene="fdeC"
                     /locus_tag="POB34_002706"
     CDS             complement(<1..1195)
                     /gene="fdeC"
                     /locus_tag="POB34_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
                     /protein_id="HDI5919617.1"
                     /translation="MSHYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLAKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEA"
     gene            2310..2411
                     /locus_tag="POB34_002707"
     CDS             2310..2411
                     /locus_tag="POB34_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919618.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            2775..3038
                     /locus_tag="POB34_002708"
     CDS             2775..3038
                     /locus_tag="POB34_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDI5919619.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            3038..3178
                     /gene="ykgO"
                     /locus_tag="POB34_002709"
     CDS             3038..3178
                     /gene="ykgO"
                     /locus_tag="POB34_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HDI5919620.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(3213..3440)
                     /locus_tag="POB34_002710"
     CDS             complement(3213..3440)
                     /locus_tag="POB34_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919621.1"
                     /translation="MQAAPQREETEWRVQSKRGLMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            4263..4805
                     /gene="ecpR"
                     /locus_tag="POB34_002711"
                     /gene_synonym="matA"
     CDS             4263..4805
                     /gene="ecpR"
                     /locus_tag="POB34_002711"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HDI5919622.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLSTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKIMDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            4880..5467
                     /gene="ecpA"
                     /locus_tag="POB34_002712"
                     /gene_synonym="matB"
     CDS             4880..5467
                     /gene="ecpA"
                     /locus_tag="POB34_002712"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HDI5919623.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFTII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            5525..6193
                     /gene="ecpB"
                     /locus_tag="POB34_002713"
                     /gene_synonym="yagY"
     CDS             5525..6193
                     /gene="ecpB"
                     /locus_tag="POB34_002713"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HDI5919624.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            6205..8730
                     /gene="ecpC"
                     /locus_tag="POB34_002714"
                     /gene_synonym="yagX"
     CDS             6205..8730
                     /gene="ecpC"
                     /locus_tag="POB34_002714"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HDI5919625.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            8720..10363
                     /gene="ecpD"
                     /locus_tag="POB34_002715"
     CDS             8720..10363
                     /gene="ecpD"
                     /locus_tag="POB34_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HDI5919626.1"
                     /translation="MRVNLLITMIIFALIWPVTELRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            10332..11042
                     /gene="ecpE"
                     /locus_tag="POB34_002716"
     CDS             10332..11042
                     /gene="ecpE"
                     /locus_tag="POB34_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HDI5919627.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            11355..11684
                     /locus_tag="POB34_002717"
     CDS             11355..11684
                     /locus_tag="POB34_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDI5919628.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(11932..12546)
                     /locus_tag="POB34_002718"
     CDS             complement(11932..12546)
                     /locus_tag="POB34_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HDI5919629.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            12964..13653
                     /gene="paoA"
                     /locus_tag="POB34_002719"
     CDS             12964..13653
                     /gene="paoA"
                     /locus_tag="POB34_002719"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit"
                     /protein_id="HDI5919630.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            13650..14606
                     /gene="paoB"
                     /locus_tag="POB34_002720"
     CDS             13650..14606
                     /gene="paoB"
                     /locus_tag="POB34_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HDI5919631.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFAQVSVAAIIHPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDTLFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            14603..16801
                     /gene="paoC"
                     /locus_tag="POB34_002721"
     CDS             14603..16801
                     /gene="paoC"
                     /locus_tag="POB34_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HDI5919632.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEGTPDK
                     NVGDFDGAFSSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPTIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQVDPADP
                     TRRFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEPNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACVKLREMIASAVGFDPEQSQFADGKITNGTRSAMLHEATAGGRL
                     IAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            16811..17767
                     /gene="paoD"
                     /locus_tag="POB34_002722"
     CDS             16811..17767
                     /gene="paoD"
                     /locus_tag="POB34_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HDI5919633.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARDAHTLALSVLAEVASVRLHQEEDSCLPPSS"
     gene            17818..18156
                     /locus_tag="POB34_002723"
                     /pseudo
     CDS             17818..18156
                     /locus_tag="POB34_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308332.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            18445..19722
                     /locus_tag="POB34_002724"
     CDS             18445..19722
                     /locus_tag="POB34_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI5919634.1"
                     /translation="MRYKPNQYIICDSYGNYYLRITLPVYMQPFFEGKRTFVRSLHTS
                     NLRVARRKRDQIADEYHCLRESVAPVNSTIENTLELLRSKAKYAKTATRMQDTASSCP
                     SLLKILEIYLTINSTKKKPATLAKARKAVEMFLSYRKKPDIALQDVSRTTVTGWIEHM
                     QKTLSQQSIANYISPMAQLWELASSRYHDAPERALSPWRGHRLDVAQSRESYEAFSNK
                     ELLQVLQVFSGNSAENKEMIALCLIGAYTGMRINEIASLTIDDVKEIEGVLCFEITQG
                     KTKAAARVVPVHSLITPLVLSLREKPHNGFLFYHASITERADGKRSTWHTQRFTRAKR
                     KALGEKGTERKVFHSLRHGVAQLLDRNQIPEDRIALLLGHTRGNTETFRTYSKNAASP
                     VELKKYIELLRYPEIEKGLSINKKSNLRRKTTP"
BASE COUNT         5190 a         5044 c         4916 g         4657 t
ORIGIN      
        1 gcgcttccca ctgcacattt ttcagtccgt gagttgcttt gctgaccaca agccccaggg
       61 aaagtgtctg accaccctta ccttcaatac gttcaggcag agcaatacgg atcacttcag
      121 atttgcggta ctcaagaacg atgttgttat tacgctcaac caggtcatag cggctgcctg
      181 ccagtacccg acgctcgcga atgctgtctg tatcgagttg tttcgccaga ggttcgccaa
      241 tccgataatt aacttccagg ccaaagcgag tgtcattctc accactcttg ccctgcttat
      301 gcccggcgct cagtgtcaga agaggcactg gcgtataggt cacctcggca gaaatagcat
      361 gcgggtcttt ctggcgctta tctttaccaa acagcccgac ttcatcgcca taatactgtt
      421 catacatcag gcttgcgcca agctgcggcc aggcgggtaa atagccctca gcacgaatat
      481 cccagccatt cgccgggcgt tcctgataat cctcaacatc cggcgatttt ttccagccag
      541 aagcccggat ataaccattg gcgctcagtt tcagataatc gcgccagtat tccgcaccaa
      601 caccaatgcg ggtatgacta cgggataaat catggtcgat aaaggtgttc acccccgcca
      661 tccagtcatt tcctgaaaaa tgacgccagc caaaaccaat atttgactga gtacgatcgt
      721 cggtacgatg tattgctccc tgagtgaaca acatatttgt tggcgtatca taaatcggat
      781 aaagcatttc cagcgaagaa tccttcagcg agaaatcttt atcgacattc agtttgacgc
      841 gcgcagtacc atatttcccg agccactcct gtatttcctg gttagcttta gctgtggcca
      901 ttccggtaat aaagttacgt gtcgcatcgc tatctggctg actgcttaaa aatgtcccgg
      961 catttgcggc aaacgacgcg acatttttct ccacgttatt atcagcagtt accgtagtat
     1021 ttcccatgct caaccgtggc tgaaccgcat gctgcgcacg tgccgccatt actggggtaa
     1081 aggtgacagc gagtggaaaa agaacctgaa cagagatatt tgcccacgcc acgcagcggg
     1141 ccagaactga ataacgaaat cgtggttgtt tatgacctgt tttataatgt gacattgaga
     1201 taatttcctg atgaacgaat taacgaacat aggcagacac aaataatggc gtctgcttgt
     1261 cagaaaaacc ggggatttcc cgatttcctt ttccgcagaa aaggaaatac ctttatgtaa
     1321 gaaagtgcac cgccacctaa tatatccagt gcctgtagcc actcatcagg tggttgggcg
     1381 cgggattata tttttattta tgggggaaaa tttagctttt tgtgcaagtc attaaataga
     1441 tacctaagtt atatttctta ctctgcggac agtgaataat tcactattca gagtgaataa
     1501 caatgacctg cctttaatta aaactattgg aaatacgtcg aaataggtat atatgccagg
     1561 ataaaccaat atatttttta tggatattca ttttccttca tcggactgta aattgcggag
     1621 atatcgaact tgcactaccc atcgcaacac tcaaacagat ttatgaattt cgttcataac
     1681 ccctgacaaa ttctgccgtc taatcgagct tttcccttta cgttatgctt ctcatgaacg
     1741 ataacacaac ttgttcatga attaaccatt ccggataaac tatgggatca aattcaggca
     1801 tcgaaaggaa ctggctggtt tccgttaatg gattaaatat ctattattct cccgagagta
     1861 aaactatcaa atcaatgagt aaccgataca gcctcacaaa ttctttcaga taattaaccc
     1921 gtcactgcgg cacgttctct cataaggcgt gcctgcagtg taatccagta tgtatccggc
     1981 atagttgacg gtttaataga actcaattcc tctcccaggt caccaacaac gatatcttgt
     2041 ataacacccc aggagatacg acattatgat gagatgcgat atgctcctca gcaccaatca
     2101 acgcccggac tgggccagtc ttgagatcag actcattccc ggaactgact tcaggcgaca
     2161 tatatcaatg acgttggtgc aataaaagat agttgatgta atacatctat tcaaatttgt
     2221 gatacatgtc aaaaaattgg ttgaccaaac tcgttattta tataagggca cttacgaagt
     2281 gcactctttt ttaaagcgag gaagtaccaa tgaaagagaa taaagtacag caaatcagtc
     2341 ataaactgat taatatcgtt gtttttgtcg caattgtaga atacgcctat ttatttctcc
     2401 atttctatta ataacggaaa taaactgttc acttcagtga tatttaaaat atgcatcctc
     2461 tccctttttt gtaagtaatt attatatccg tgggagagga atacacgctg tcaggtaatc
     2521 aatcatactg cgataaatca tcggccagta aagtggagat aacctccatt ctcgaaaaat
     2581 ccatactctc agcaaaacca tcatcaatca ctcatccagg cgtttatggg agcgtcacca
     2641 atggctgcta acaatgccag agttccccgt tgcgaaaatt ccacatccac aaagagtcac
     2701 agggattgag tgttgaaatg atccggatga gcatgtatct ttatggttat gttataacat
     2761 aacaggtaaa aatgatgaag cccaatatcc atcctgagta tcgtactgtg gtgttccacg
     2821 acaccagtgt tgatgagtac tttaaaatcg gctcgactat caaaacagac cgtgagattg
     2881 agctggatgg cgtaacgtat ccatacgtga caattgatgt ctcttctaaa tcgcacccgt
     2941 tctatacagg gaagctgaga acagtggcat cagaaggaaa tgttgcacga ttcacccaac
     3001 gttttggtcg ttttgttagc acgaaaaagg gggcgtgatg aaagttctta actctctgcg
     3061 taccgcaaaa gaacgccatc cagactgtca gattgtgaag cgaaaaggac ggctatatgt
     3121 gatttgtaaa tctaatccac gttttaaggc cgttcagggt cgtaagaaaa aacgttgatt
     3181 caaaattcga cggattaacg atatttgtct gattaataat cagatcggat taatgttggt
     3241 gtgtttataa caccaacatt aattttcctg gggatatatt cttcctgttc atttgaggcc
     3301 aactgcctga cgtttctctc cgaatattcc attatcttaa tgttgacttg ttgaccagct
     3361 tcgcccctgt atgctggcat caaccctctt ttagactgaa cacgccactc agtctcctcc
     3421 ctttgcggcg cagcctgcat tttcactcaa actgttaaga tgataaatgt ggtaaatctg
     3481 ttggtactaa cataaaaacg tttacgccac aggaacagtc tgatccaccg gtaaccccgt
     3541 cgccgacgtt cgagtgccag ttagagtaac gcgcacagat aactgaatgc agtgccctga
     3601 caaaaaggcc atcgttcctg tgacagctgg cagccttcgt ttaacttcac ttaatctggc
     3661 tcttgggggc ttaccgaaca gatgacgtac atacgcccgt tcaattttcc attacttatt
     3721 ggaatgaaca cctgtaacca ttttgtgcgg catgttaatc cattaaaata tcttactgat
     3781 tggcaaatca tcttcaatga cagctcatca tagttttata ttctatccct tacccttaaa
     3841 acttgttttt ttactagtcc atcacacagc gcattaagac tattcctaac acttcagggc
     3901 aaagttcctg accaatataa aatgcaagta agaattgaac gttatattgc caataacctt
     3961 atgaaaccaa atgtcttttt cttcttatca aaaaagcaat attttcagtt tttctaaata
     4021 ttgacttaac cattgaattc cttttccgtt cacatattga cactcatcgg gaaaaaaaca
     4081 taaatttaag cccaatcgaa aataattaaa cttaatctcg tttaaccttt attgatatgt
     4141 actacgtatc ttatttactt ccggtttact aaggaaactg aatgcacctg taaaaattac
     4201 aggtttggaa agtagtgaca tggcaaagtg attacagtag ggactatgag gttaaaaacc
     4261 atatggaatg tcaaaaccgt tctgataaat acatctggtc tccccatgac gcctacttct
     4321 ataaaggact atctgaactg attgtggata tcgacagatt aatttatcta tcgttggaga
     4381 aaattagaaa agatttcgtg tttatcaatc tcagtacgga ttctttatct gaatttataa
     4441 accgtgataa tgaatggtta tccgcggtaa aggggaaaca ggtcgtattg attgcggcca
     4501 gaaagtcaga agccttagca aattattggt attacaatag caatattagg ggcgtggtat
     4561 acgctggact gagtcgtgat attagaaaag aactggccta tgtgattaat ggcaggttcc
     4621 tgagaaaaga tattaagaaa gataaaatca tggaccggga aatggaaatt atccgcatga
     4681 cggcccaggg aatgcaacct aaatcgattg ccagaattga aaattgtagt gtgaagacag
     4741 tgtataccca tcggcgtaat gctgaggcca agctgtactc aaaaatatat aagttggttc
     4801 agtaaactcc aggcaagtta gttttaaaaa atgactcact gggacatcac gtcctcaatt
     4861 caactcggga agaaatgcaa tgaaaaaaaa ggttctggca atagctctgg taacggtgtt
     4921 taccggcatg ggtgtggcgc aggctgctga cgtaacagct caggctgtag cgacctggtc
     4981 ggcaacagcc aaaaaagaca ccaccagtaa gctggttgtg acgccactcg gtagcctggc
     5041 gttccagtat gccgaaggca ttaaaggttt taactcacag aaaggtctat ttgacgtggc
     5101 tatcgagggt gactcaacgg ctaccgcctt taaactgacc tcacgtctta tcaccaacac
     5161 attaacccag ttggatacct caggttccac actgaatgtg ggcgtggatt ataacggcgc
     5221 ggcagtcgaa aaaactggcg ataccgtgat gatcgatacc gccaacggcg tactgggcgg
     5281 caaccttagc ccgctggcta acggttacaa tgccagcaat cgtaccaccg cacaggatgg
     5341 tttcaccttc accatcatca gcggcaccac caatggtacc accgcagtaa ccgattacag
     5401 cactctaccg gaaggcatct ggagcggcga cgttagcgta cagttcgacg cgacctggac
     5461 cagttaatct ctctgatgta ccagcagggg tagcccccct gctttattcc tggacggaat
     5521 tgttatgaaa aagcaccttc tgcctctcgc tctgctgttt tccggaatat ctccggccca
     5581 ggcgctggat gtcggcgata tatcatcgtt tatgaacagt gacagcagca cgctgagcaa
     5641 aacgatcaaa aacagtaccg acagtggtcg ccttatcaat atccgtctcg aacggctctc
     5701 ttcaccgctt gacgacgggc aggttatctc aatggacaag ccggatgagt tgctactcac
     5761 tcccgccagc ttgctgctac ccgcccaagc cagcgaagtg atccgcttct tctataaggg
     5821 acccgcagat gaaaaagagc gctactaccg cattgtctgg tttgatcagg ccctcagtga
     5881 tgcacagcgc gataatgcca accgcagcgc tgtggccact gcttccgccc gcatcggcac
     5941 cattctggtc gtcgcacccc gccaggcaaa ctaccacttt cagtacgcca acggctccct
     6001 gacaaataca ggaaatgcga cgctgcggat cctcgcctac ggcccctgcc tgaaagccgc
     6061 caatggtaaa gagtgtaaag agaattacta cctaatgccg ggcaagtcgc gtcgttttac
     6121 ccgcgtggac acggcggata acaaaggacg ggttgcactt tggcagggtg ataagttcat
     6181 tcccgtgaaa tagatagctc gacaatgcct ttacgacggt tctccccagg actgaaagcc
     6241 cagtttgcct tcggcatggt ctttttgttc gttcagcccg atgccagcgc tgctgacata
     6301 agtgcgcagc aaataggtgg ggtgattatt ccgcaggcct tcagtcaggc gcttcaggac
     6361 ggcatgagcg tcccgctcta tattcatctc gccggtagcc agggtcgcca ggacgatcag
     6421 cgaatcggca gcgcttttat ctggctggac gatggacagc tacgcatccg gaaaatacag
     6481 ctggaagaga gtgaagataa cgccagtgtc agcgaacaaa ctcgacagca gctgatggct
     6541 ctggcgaacg ccccgttcaa tgaggccctt accatccccc tgactgacaa cgcgcagctg
     6601 gatctcagct tgcgccaact gctgctgcag ctggtggtca agcgcgaagc gctgggcacc
     6661 gtactacgct cacgtagcga agacatcggg cagtccagtg ttaacaccct cagcagtaat
     6721 ctgagctata acttgggcgt ctataacaac cagttgcgta acggcgggag caacacatcc
     6781 agctatctgt cgctgaataa cgttactgcg ctgcgcgaac atcatgtggt gctcgacggc
     6841 tcgctgtacg ggatcggtag cggtcaacag gacagtgaat tatataaagc gatgtatgaa
     6901 cgcgattttg ccggtcaccg atttgccggt ggaatgctcg acacctggaa cttgcagtcc
     6961 ttagggccga tgaccgccat ttcagcaggg aagatttacg gcctttcctg gggaaaccag
     7021 gccagctcca ccatcttcga cagcagccag tcagccacgc cagtgatcgc ctttttaccg
     7081 gcggcgggcg aagtacatct cacccgtgat gggcggctac taagcgttca gaacttcact
     7141 atgggcaatc atgaagtgga tacccggggt ctaccgtacg ggatttacga tgtggaagtt
     7201 gaggtgatcg ttaacggtcg cgtgatcagc aaacgcaccc agcgggtcaa taagctgttt
     7261 agccgggggc gcggcgtcgg tgcaccactg gcgtggcagg tatggggcgg tagctttcat
     7321 atggatcgct ggtcggaaaa cgggaaaaag acgcgaccag ctaaagagag ttggctagca
     7381 ggtgcctcga cctccggctc attgagtacg cttagctggg cggcaacggg atatggatac
     7441 gataatcagg cggtgggtga aacccgtctg acgctgccgc ttgggggggc gatcaacgtt
     7501 aacctgcaaa atatgctggc cagtgacagc tcatggagca gcatcggcag catcagcgcc
     7561 actctaccgg gaggctttag ttcgctgtgg gttaatcagg aaaaaacccg cattggcaat
     7621 caattgcgac gtagcgatgc cgataaccgt gcaatcggcg gcacactcaa cctgaactca
     7681 ctgtggtcga agctgggcac attcagcatc agctacaatg atgaccgccg ttacaacagc
     7741 cattattaca cggcagatta ctatcaaaat gtctacagcg gtacctttgg ttcgcttggc
     7801 ctgcgggccg gtattcagcg ctataacaac ggcgacagca acgccaatac agggaaatat
     7861 atcgctctcg atctctcgct accactgggc aactggttta gcgcagggat gacccatcaa
     7921 aacggctaca ccatggcaaa cctgtcagca cgcaaacagt ttgatgaagg aaccattcgc
     7981 actgttggtg ccaatctgtc acgtgccatc tccggcgata ccggtgatga caaaaccctc
     8041 agcggtgggg cgtatgcaca gttcgacgct cgttacgcca gcggaacgct gaacgtcaat
     8101 agcgcggcgg acggctacat caataccaac ttgaccgcca atggcagcgt cggctggcag
     8161 ggtaaaaaca tcgctgccag cgggcggact gatggcaacg ctggggtgat attcaacacc
     8221 gggctggagg acgacggtca gatcagcgcc aaaatcaacg ggcggatttt cccgcttaac
     8281 ggcaagcgta actatctccc gctctctccc tatggaagat atgaggtgga gttacagaac
     8341 agcaaaaact cactcgacag ctacgatatc gtcagcggcc gcaaaagcca tctgactctc
     8401 tatccaggca atgtcgctgt cattgagcca gaggtgaagc agatggttac cgtctccggt
     8461 cgtatccgtg cggaagacgg cacactgctg gctaacgcac ggattaacaa ccatatcggc
     8521 cgaacccgaa ccgatgaaaa cggcgagttt gtcatggacg tggataagaa atatcccact
     8581 atcgattttc gctacagtgg caataaaacc tgcgaagtgg cactggaact caaccaggcg
     8641 cgcggtgccg tctgggtcgg tgatgtggtc tgcagcggcc tctcatcgtg ggcggcggtg
     8701 acgcagacag gagaagagaa tgagagttaa cctactaata acgatgataa ttttcgcgct
     8761 aatctggcca gtaactgagc tcagagcggc agtgagcaaa acaacctggg cggatgcacc
     8821 ggcacgcgag tttgtgtttg tcgaaaacaa ctcagacgac aactttttcg tcactcctgg
     8881 cggggcgctg gatccgcgcc tgaccggtgc caaccgctgg accggtttaa aatacactgg
     8941 ttcaggaacc atctatcagc aaagcctcgg ctacattgat aacggttaca acaccggcct
     9001 ttataccaac tggaagtttg atatgtggct ggaaaattca ccagtttcat ctcctttaac
     9061 tggcttgcgc tgcatcaact ggtacgctgg gtgtaatatg accaccagtc ttatcctgcc
     9121 gcaaaccacc gacgccagtg gattttatgg cgcgacggtg accagcggcg gcgcgaagtg
     9181 gatgcacggc atgttgtcag acgcgtttta ccagtatctg caacaaatgc ccgtcggcag
     9241 cagctttaca atgaccatca atgcctgcca gacctctgtg aactatgacg ccagcagcgg
     9301 cgcacgctgt aaggatcagg cctccggcaa ctggtatgtt cgcaacgtca cccatacgaa
     9361 agcagcaaat ctgcggttaa taaataccca ctcgctggcg gaggtattta tcaacagcga
     9421 cggagtaccg actctgggcg aagggaacgc cgactgccgg acacagacca tcggcagcct
     9481 ttcaggatta agttgtaaga tggtcaacta taccctgcaa acaaacggac tcagcaacac
     9541 ctcaatccat atattcccgg cgatcgccaa ctcgtcgtta gcctcggccg tcggggcgta
     9601 cgatatgcag ttcagtctga atggcagttc atggaaaccg gtgagcaata ccgcctatta
     9661 ctacaccttc aacgagatga agagcgcaga ctcgatctat gttttcttct cgagcaactt
     9721 ctttaagcag atggtgaacc tcgggatcag cgatatcaac accaaagatc tattcaactt
     9781 tcgctttcag aacaccacat caccggagtc tggctggtat gaattttcta cctccaacac
     9841 gctgattatc aaaccccgtg atttcagcat cagtattatc tccgatgaat atactcagac
     9901 accgtcgcgg gagggatatg ttggcagcgg cgagtcggca ctcgatttcg gctatatcgt
     9961 aaccaccagc ggtaaaacag ctgccgacga agtgctgatc aaggtgaccg gacccgcgca
    10021 ggtgattggc gggcgctcct attgtgtctt cagctccgat gacggtaagg cgaaagtacc
    10081 gttcccggcg acactttcct ttattacccg caacggagct acaaaaacct acgatgccgg
    10141 gtgcgatgat agctggcggg atatgaccga tgcgctgtgg ttgaccacac cgtggactga
    10201 tatctctggc gaagtggggc agatggataa gaccacagtc aaattttcga ttccaatgga
    10261 taacgccatt tcgctgcgta cggtagatga taacggctgg tttggcgaag tcagcgcttc
    10321 aggagagatt catgttcagg cgacgtggcg taacattaac taaggccctg ctgacagcgg
    10381 tctgtatgct ggcggcacct ttgacacagg cgatttcggt cggcaatctg acattttcgc
    10441 tgccgtccga gactgacttt gtcagcaaac gtgtagtgaa taacaacaaa agcgcacgga
    10501 tataccgtat tgccatcagt gctattgata gcccgggcag cagtgaattg cgcacccgac
    10561 cggtggatgg tgaactgctt ttcgcccccc gccaactggc gttgcaggct ggtgagagcg
    10621 agtattttaa attttactat catggtccac gggataaccg cgagcgctac taccgggtct
    10681 catttcgcga agtccccact cgtaaccaga caagacgtag cccaaccggc ggcgaggtca
    10741 gcacggagcc ggtggtggtg atggatacca ttctggtagt acgaccacgt caggttcagt
    10801 ttaaatggtc cttcgacaag gtgacaggaa cggtaagtaa cactggcaac acatggttta
    10861 agctactgat taagccagga tgtgattcga ccgaagagga aggcgatgcc tggtatctac
    10921 gtccgggaga cgtggttcat cagcctgagt tacgtcagcc ggggaatcat tatctggtct
    10981 ataacgacaa attcattaag attagcgatt cctgtccggc taagccccct tcggcggact
    11041 aagacttatc catcggtcga gggaagaatt ccacctcaga gctccaaaaa tcgtttaaat
    11101 gatgtggaag cgatcgtcaa tgtcacctgt aacgatgggc tttctcatat gcacaccata
    11161 gtgttcagga gacacggaaa ggtatcaggc agcctgctaa cgagtggttt aatcacgaat
    11221 tagtacgtaa aatcggtaac ggctggaaat cattcaatac tcacactatc gaaagttccc
    11281 cagccaaccg cggtacgttc ttacatacga tgtaccgctg ttctctttac gatttttagc
    11341 tgtactggtg aattatgagc aatctgaatc catgcatgac gtgtggtgcc tgttgtgcat
    11401 ttttccgcgt ctctttttac tgggccgaag ccgacgatgc tggcggtact attcccgcca
    11461 ggctcactga acaaatatcc ccttttcacc gatgcatgag cggtaccaat cagaaaaacc
    11521 cccgatgtat tgcccttgca ggaaccccgg gcaaaaatgc ctgctgcacg atatataaaa
    11581 atcgatcgtc cacatgcaga gaattcgcca tgtctggtga gaacggagtc gtcaatgagg
    11641 cttgcaatcg tgcaagggct aaatacgggc tgacaccgtt ataaacatac aataattaat
    11701 tgcactgccc cgccagcgat aatagcgggg cttcgttttt caggggtaac aaaacccgat
    11761 acttcttcta tttgccagca acaatgcctc tcttctgtag cgttcttcgg atctacatca
    11821 tcctgaagta gcgcgatata ctcactgact atctgcatca gaatataaaa agcaatgttt
    11881 ttaacctata aaaatggcgc tgtatttgcg ccatttttat cattcaatgc attatctgtt
    11941 tgagcctaaa gggatctcag ggtctggctc atgagtaatt ctgtttcgta aatctctgcg
    12001 aataatttca atagaccaga accagactaa atgtccaaaa atttcagaaa cattctcata
    12061 ccacgggaga tcaaacagag gtggcgtcag tcccatgaga gggaatgaaa tcatatgaac
    12121 aaaaagttgg gctaaagcac ctgccagtaa gccctgccag agtttaattt ttggaaatac
    12181 ttcagcgacc acacaataac cgacagcaaa cactatcgaa aagataatgt gcgtaacacc
    12241 aacccagtta aagacatgcc cggcaaaggt ataaacagcc gcattgggat ctgtcagccc
    12301 caaccagtct cgaagaaaaa tatacggtgg attgagaaaa ttacgcgagc aatcaatttg
    12361 gcctgcagcc ctgattaatg attccgggcc acacgctgca ttaaacatat ccaccgggct
    12421 acgtggcggc aatggaactt cagcccccca cttcacgaat gcggaaacaa cgccagcaat
    12481 cagcccaatg aatgcagcaa gaccataacg tctgcggttc ggtggagttt gttcaaatat
    12541 attcatatct accctgcttg taccattatg ttatacacct cttcaggagt attcataaaa
    12601 caaggcaaat gtaaagaact gtattgtttt gtataacaag atggtttcct aatcgccaat
    12661 gaatataagc tccatcattt ctccctattt ttatattaaa agtgacagag atttgcaggg
    12721 tgatgcagag ctgaaaatca caggtttcct tattggtttt tgcatcgtac aactaaagca
    12781 ataaaccagc tcccatcatc atcaacatcc cggcgaacat gaatttatta gccgggctca
    12841 tagctgcatt cgcgttttaa gaataatcct cctgctgtcg ccgactatgc ttaacattta
    12901 aaaaagcatc agcactctcg caacgcactc ttattttccc ctttagaata ccggaggcct
    12961 ggtatgagca accaaggcga ataccccgaa gataatcggg ttgggaagca cgagccgcac
    13021 gatttaagtt tgacccgtcg cgatctgatt aaagtgagcg ccgcaacagc ggcgaccgcc
    13081 gtggtttatc ctcattctac gctggcggca agcgttccgg cagctacacc cgcgccagag
    13141 ataatgcccc tgacactgaa ggtgaacggc aaaaccgagc agcttgaggt ggatacccga
    13201 accacgctac tggacgcttt gcgtgaaaat ctgcatttga tcggtaccaa gaaaggttgc
    13261 gatcacggac agtgcggagc ctgtaccgtg ctggtcaatg gtcgcaggct taatgcctgc
    13321 ctgacgcttg cagtcatgca tcagggggcc gagatcacca ccattgaagg cctgggctcg
    13381 ccagataatc ttcaccccat gcaggcggcc tttatcaagc atgatggctt ccagtgcggc
    13441 tactgcacct ccgggcaaat ttgctcatca gtagcggtgc taaaagagat tcaggacggc
    13501 attcccagtc acgtcacggt cgatttggtt tccgctccag aaacaactgc cgatgagatc
    13561 cgtgaacgta tgagcggcaa catctgtcgc tgtggtgcat acgctaacat ccttgccgcc
    13621 attgaagatg ctgcggggga gataaaatca tgaaggcgtt tacctatgaa cgagtgaaca
    13681 ccccagccga ggcggcactt agcgctcagc gcgtacccgg cgcaaaattt atcgcgggcg
    13741 ggaccaatct gctggacctg atgaagctgg aaattgaaac gcccacccac cttatcgatg
    13801 tgaacggcct cgggctcgat aagattgaag tgaccgacgc gggtgggctg cgcatcggcg
    13861 cactggtacg gaacaccgac ctggcggctc acgagcgcgt gcgtcgtgat tacgcggtac
    13921 tctcccgcgc cctgctcgct ggcgcatctg gtcagttacg taatcaggca accaccgcag
    13981 gtaatctgct ccagcgcacg cgctgcccct atttttacga caccaatcag ccctgcaata
    14041 agcgcctgcc cgggagcggc tgcgcggcgc ttgaaggctt tagccgtcag cacgcggtgg
    14101 taggcgtaag cgaagcctgc attgccaccc atccgagcga tatggcggtc gcaatgcggt
    14161 tgctggatgc ggtggtggaa accatcacgc cggagggaaa gactcgcagt atcacactgg
    14221 ctgattttta tcaccctccg ggaaaaacgc cgcacattga aaccgccctg cttcccggtg
    14281 agcttatcgt tgcggtgacg ttacctccgc cgctcggcgg aaaacatatc taccgtaagg
    14341 tgcgcgatcg cgcctcctac gcctttgccc aggtatcggt cgcggcgatt attcaccctg
    14401 acggcagcgg gcgcgtcgcg ctgggcggag tagcacataa gccctggcgc attgaggctg
    14461 cggatgctca gctatcccag ggggcgcagg ccgtatatga cacgctgttc gccagcgccc
    14521 atcccaccgc tgaaaacacc tttaaactcc tgttggcgaa gcgaacgctt gcctccgtac
    14581 tggctgaagc gagggcacag gcatgaaatt tgataaaccc gcaggggaaa acccgatcga
    14641 tcagctgaag gttgtcggtc gtccccatga ccgcatcgac ggaccgctga aaactaccgg
    14701 cacggcacgc tacgcctacg aatggcatga agaggccccc aacgccgcct atggctatat
    14761 cgtcggttcc gccattgcca aaggacgcct caccgccctt gatacggacg ccgcgcaaaa
    14821 agcgccgggc gtactggctg tcattaccgc cagtaacgcc ggggcactcg gcaaaggcga
    14881 caaaaacacc gccaggctgt taggcggccc cactattgag cactatcatc aggccattgc
    14941 gctggtagtg gccgagacct tcgaacaggc gcgagcggcg gcctcgctgg tgcaggcgca
    15001 ctatcgccgt aataaaggag cttactccct ggcggacgaa aaacaggccg tcaatcagcc
    15061 gccggaaggc acgcccgaca aaaacgtcgg tgactttgac ggggctttct cctccgctgc
    15121 ggtgaagatt gatgctacct acacgacccc ggaccagagc catatggcga tggagccgca
    15181 tgcctcgatg gccgtctggg atggaaataa gcttactctc tggacctcaa atcagatgat
    15241 tgactggtgc cgcaccgatc tggcaaaaac gctgaaagtg cccgtggaga atgtgcgtat
    15301 tatctccccg tatatcggcg gagggtttgg cggcaagctg ttcctgagaa gcgatgcgct
    15361 gctggcagcc ctcgccgccc gagcggtgaa acgtccggtt aaagtgatgc tcccccgccc
    15421 cactattccc aataacacca cgcaccgccc cgcaaccctt cagcacctgc gtattggtgc
    15481 tgaccagagc gggaaaatca ccgctatctc acatgaaagc tggtccggaa acctgcccgg
    15541 cggcacgccg gaaacggcgg tacagcaaag cgaattactc tacgccgggg caaaccgtca
    15601 taccggcctg cggctcgcca cgcttgattt gccggaaggg aacgccatgc gtgcgcccgg
    15661 cgaagccccc ggtctgatgg cgctcgaaat cgcgatcgac gaactggcgg aaaaagcggg
    15721 catcgatccc gtcgagtttc gcatcctgaa tgacactcag gttgaccccg ccgacccgac
    15781 gcgccgcttc tctcgccgtc agcttatcga gtgcttgcgc accggagcgg ataaatttgg
    15841 ctggaagcag cgcaacgcca cacccggaca ggtgcgcgac ggggagtggc tagtcggcca
    15901 cggcgtcgcg gcgggctttc gcaataatct gctggaaaaa tcgggggctc gggttcacct
    15961 cgaaccaaac ggcaccgtta ccgtggaaac ggacatgacc gacattggca ccggcagcta
    16021 caccattctg gcccagacgg cagcggaaat gcttggcgta ccgctggagc aggttgcggt
    16081 tcacctcggc gattccagtt tcccggtttc tgcgggttct ggtggacaat ggggcgcgaa
    16141 tacctccacc tccggcgttt acgccgcctg tgtgaagctt cgcgaaatga ttgcctcggc
    16201 agtcgggttt gatcctgagc agtcgcagtt tgccgacggc aagattacca acggtacccg
    16261 aagcgccatg ctacatgagg ccaccgcagg cggcagactg atagcggaag agagcattga
    16321 attcggaaca ctgagcaagg agtaccagca gtcgaccttt gccgggcatt ttgtggaggt
    16381 cggcgtgcat agcgcgacgg gagaagttcg ggtccggcgt atgctcgctg tgtgtgctgc
    16441 aggacgcatc ctgaatccga aaactgcacg cagccaggtc attggcgcaa tgactatggg
    16501 catgggcgcg gcactgatgg aggagctggc ggtggatgac cgtttgggct acttcgttaa
    16561 tcacgatatg gcggggtatg aggtgccggt ccatgcggat atcccaaaac aggaggtgat
    16621 tttcctggat gataccgacc ccatatcctc cccgatgaag gccaagggtg tcggtgagct
    16681 gggcctgtgc ggcgtgagcg cggctatcgc caacgcggtg tataacgcca ccggtattcg
    16741 ggtacgcgat tatcccatca ctctggataa gctgctcgat aagctgccgg atgtggttta
    16801 aggagaaaca atgtcatacc cgctttttga taaagacgaa cactggcata agccagagca
    16861 ggcgtttctc accgatgacc accggaccat tctgcgcttc gccgtagagg cgctaatgtc
    16921 cggtaaagga gctgtgctgg tgacgctggt ggagatacgc ggcggcgcgg cgcgcccgct
    16981 cggggcgcag atggtggtgc gcgaagatgg tcgttactgc ggttttgtct ctggcggctg
    17041 cgtggaggcc gctgccgctt ttgaagcgct ggagatgatg ggctcaggcc gcgatcgcga
    17101 aattcgctat ggcgaaggtt cgccgtggtt tgacatcgtt ctgccctgcg gcggtgggat
    17161 cacgctgacg ctccataaac tacgctcggc acagcctctg ctcgccgtgc tgaaccgcct
    17221 ggaacagaga aaaccggcgg ggctgcgcta cgatccgcaa gcacaatcgt tggtgtgcct
    17281 gcccacgcaa acccgaacgg gctggaatct caatggcttt gaggtggggt tcaggccatg
    17341 cgtcaggctg atgatttacg gacgttctct tgaggcgcag gcaaccgcga gtcttgcagc
    17401 agccacaggc tatgacagcc atatcttcga tctttttccg gcctcagcca gcgctcagat
    17461 cgataccgat acggcggtca ttttgctgtg ccatgatctc aaccgggagc tgccagtgtt
    17521 gcaggccgcg cgagaagcaa aaccctttta tctcggcgca ttgggcagct atcgaaccca
    17581 cactttacgt ctgcaaaagc tccacgagct gggatggtcc agggaggaga cagcgcaaat
    17641 ccgggcaccc gtcgggatat ttcccaaagc ccgggatgcg catactctgg cactctccgt
    17701 gctggcagaa gtcgcctctg tacgtctcca tcaggaggag gattcatgcc tgcccccgtc
    17761 gtcctgatcc ttgcggccgg gcgtggagag cgctttctcg cctccggggg aaatacccat
    17821 aagtgtatcg gctggcgtca gtccccggag gttgcgcctt atcgctggcc atttgaagaa
    17881 aacgggagaa ctttcgacct tgcgattgaa ccgcagatta cgactaatga tctgcgtctg
    17941 atgttgcagc tggctcttgt cggtgaagga ataacaattg ccactcagga aactttcagg
    18001 ccatatattg aaagcggtaa gcttgtatcg ctgcttgatg actttcttcc acaatttcca
    18061 ggcttctatc tgtatttccc acagcgtcgc aatattgcac caaagctccg cgccctgatt
    18121 gaccacgtca aggaatggcg gcagcaattg gcttaaatgt ctgcacctgc attgcctgat
    18181 gtcagaacag tattttgatg aattgccagg gttacaatgg cacaaatacg gcacaggagg
    18241 aaacgtggtg tatttaaata tggggtaact tattgattta aatggtgccg ataataggag
    18301 taaaacactc gattaatctc gtgttaatgc catcacctac gaccattact aattgattga
    18361 atcttataga aaaaatcatc attttctatg cgttacaatc aattttcaca ttacaaacgc
    18421 tccgacactt tagccgggga gtttatgaga tacaagccta atcaatacat aatctgtgat
    18481 tcttacggta actactattt acggatcacg ctgcctgtgt atatgcagcc cttttttgaa
    18541 ggaaaaagga cgtttgtcag aagtctacac acaagtaatc ttcgtgttgc acgtagaaag
    18601 cgtgatcaga ttgcagatga ataccattgc ttacgagaga gtgtcgctcc tgtaaacagc
    18661 acaatagaga acacgctgga gctgttacgc agtaaggcta aatacgccaa aacagctacc
    18721 agaatgcaag atacagcgtc ttcgtgtccg tcattgctta aaattcttga aatctacctg
    18781 acaattaaca gcacgaagaa gaagccagcc actttagcta aggcaagaaa agcggtagag
    18841 atgtttctct cctaccgtaa aaagcctgat attgcattgc aagatgtgag ccgcaccact
    18901 gttacaggct ggattgaaca catgcaaaaa accctttcac aacaatcaat tgcaaattat
    18961 atcagcccaa tggcccagct atgggagtta gcttcatcac gttaccacga tgcgccagaa
    19021 agggcgctct ccccctggcg agggcatagg cttgatgtgg cacaaagtag agagagctat
    19081 gaggcatttt ctaacaaaga gctattgcag gtgttgcaag tattttccgg taattcagca
    19141 gaaaacaaag aaatgatagc tttgtgtctt atcggtgctt atacaggtat gcggatcaat
    19201 gagatagcaa gtctcacaat agacgatgtg aaagagatcg aaggtgtgct gtgttttgaa
    19261 atcacacagg gaaaaacgaa agctgcggcg cgtgttgtgc ctgtacatag ccttatcact
    19321 ccgttggtgt tgtcgctgcg tgaaaagcct cataatggct ttttgttcta tcacgccagc
    19381 attacagagc gagccgatgg taaacgttcc acgtggcaca cgcagagatt tacaagagct
    19441 aaacgaaagg ctttagggga aaagggaaca gaaaggaagg tgtttcattc tctgagacac
    19501 ggagtagcac agcttcttga tcgaaatcaa attccagaag acaggatcgc ccttctcctg
    19561 ggccatacac gcggcaatac agagacattc cgcacatata gcaagaatgc agcttctcca
    19621 gtagagctta aaaaatatat tgagcttcta cgctaccctg aaatagagaa aggcttatca
    19681 atcaataaaa aatcaaattt aaggcgtaaa acaacgccat agacgcaata aagaggcttc
    19741 aacatagatt ggtagcctct tttataatta catcgttaca gagcgtttta gaggtattta
    19801 cggctat
//
LOCUS       DAMZET010000076        19725 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.076, whole genome shotgun sequence.
ACCESSION   DAMZET010000076 DAMZET010000000
VERSION     DAMZET010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.076"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..87)
                     /locus_tag="POB34_002725"
     CDS             complement(<1..87)
                     /locus_tag="POB34_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HDI5919635.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPR"
     gene            complement(251..2092)
                     /gene="nuoL"
                     /locus_tag="POB34_002726"
     CDS             complement(251..2092)
                     /gene="nuoL"
                     /locus_tag="POB34_002726"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HDI5919636.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQSYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(2089..2391)
                     /gene="nuoK"
                     /locus_tag="POB34_002727"
     CDS             complement(2089..2391)
                     /gene="nuoK"
                     /locus_tag="POB34_002727"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HDI5919637.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(2388..2942)
                     /gene="nuoJ"
                     /locus_tag="POB34_002728"
     CDS             complement(2388..2942)
                     /gene="nuoJ"
                     /locus_tag="POB34_002728"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HDI5919638.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(2954..3496)
                     /gene="nuoI"
                     /locus_tag="POB34_002729"
     CDS             complement(2954..3496)
                     /gene="nuoI"
                     /locus_tag="POB34_002729"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HDI5919639.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(3511..4488)
                     /gene="nuoH"
                     /locus_tag="POB34_002730"
     CDS             complement(3511..4488)
                     /gene="nuoH"
                     /locus_tag="POB34_002730"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HDI5919640.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(4485..7211)
                     /gene="nuoG"
                     /locus_tag="POB34_002731"
     CDS             complement(4485..7211)
                     /gene="nuoG"
                     /locus_tag="POB34_002731"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HDI5919641.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQEHLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQELAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(7264..8601)
                     /gene="nuoF"
                     /locus_tag="POB34_002732"
     CDS             complement(7264..8601)
                     /gene="nuoF"
                     /locus_tag="POB34_002732"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDI5919642.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(8598..9098)
                     /gene="nuoE"
                     /locus_tag="POB34_002733"
     CDS             complement(8598..9098)
                     /gene="nuoE"
                     /locus_tag="POB34_002733"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HDI5919643.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(9101..10903)
                     /gene="nuoC"
                     /locus_tag="POB34_002734"
     CDS             complement(9101..10903)
                     /gene="nuoC"
                     /locus_tag="POB34_002734"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HDI5919644.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(10997..11659)
                     /gene="nuoB"
                     /locus_tag="POB34_002735"
     CDS             complement(10997..11659)
                     /gene="nuoB"
                     /locus_tag="POB34_002735"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HDI5919645.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(11675..12118)
                     /gene="nuoA"
                     /locus_tag="POB34_002736"
     CDS             complement(11675..12118)
                     /gene="nuoA"
                     /locus_tag="POB34_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HDI5919646.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(12749..13687)
                     /gene="lrhA"
                     /locus_tag="POB34_002737"
     CDS             complement(12749..13687)
                     /gene="lrhA"
                     /locus_tag="POB34_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HDI5919647.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            14607..15824
                     /gene="alaA"
                     /locus_tag="POB34_002738"
     CDS             14607..15824
                     /gene="alaA"
                     /locus_tag="POB34_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HDI5919648.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            15908..16507
                     /gene="yfbR"
                     /locus_tag="POB34_002739"
     CDS             15908..16507
                     /gene="yfbR"
                     /locus_tag="POB34_002739"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HDI5919649.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     ATIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(16566..18398)
                     /locus_tag="POB34_002740"
     CDS             complement(16566..18398)
                     /locus_tag="POB34_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDI5919650.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(18485..19135)
                     /gene="hxpA"
                     /locus_tag="POB34_002741"
     CDS             complement(18485..19135)
                     /gene="hxpA"
                     /locus_tag="POB34_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HDI5919651.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLSHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(19146..19640)
                     /locus_tag="POB34_002742"
     CDS             complement(19146..19640)
                     /locus_tag="POB34_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HDI5919652.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
BASE COUNT         4937 a         5476 c         4935 g         4377 t
ORIGIN      
        1 gcgtggcacc ttgacgccaa agcgttcggt ctgccagcac aggaagccgc caataaaggg
       61 aattaatatt agccagggta gtaacatggc gatctttatt ccttgtaaaa gtcccgtcag
      121 gaccggattt tcaacgaatt ctcacgacag aaaattctct tcggactggg ggtagtgtcg
      181 gatgcaccgc gaaggcggct tatccgacct acgttcttat atgcccaggg gcaatcccac
      241 aatccttaac tcaacgcagt accatcaaca gtgccagcac cacgaccgca ccgatgctca
      301 tggatgccac ataccagcgc agataaccgt tctcgcttaa cagcagacct ttacctgcaa
      361 agcgggaaag aaccgccggg atgttcatca ttgagttcag cggatcgcgt ttcagcaacc
      421 aggcaatacc caggaacggt ttgacgaaca ctttgtcgta cagccagtca aagccccagg
      481 cgttgtacca ccaggtgccc agcagacggc ccggcgcact gttggcaatg gaggtcacca
      541 gagtacgttt acccagccac agccaggctg ccagcagaat gccgaccacc gcgaccacgc
      601 cagaggtaat ttccagggtc aacatgctgc cgtgcgccag ctccgtcgtt tgcggaagca
      661 cgccctgcag cggcggtaca atcagtgcgc caacgaaggt ggaaaggatc agcagcacaa
      721 tcagcggcag gctgtgagtt acccctttca cggcgtgagc gtgaatttgt tcttttccgt
      781 ggaagacgat gaaaatcata cggaaggtgt agagcgaggt cataaacgca ccgaccagac
      841 ctgccaccat cagattgata tgaccattcg ccatcgcacc cgcgaggatc tcatccttac
      901 tgaagaagcc agcagtgacc agcggcagcg ccgacagtgc tgcgccgcct accaggaagc
      961 agagataaac cagcggaata gatttacgca gaccgcccat cttgaagatg ttctgttcgt
     1021 gatggcaggc cagaatgacg gaaccggatg ccaggaacag cagcgcttta aagaacgcgt
     1081 gagtcatcaa gtggaaaatc gccgcatccc atgcctgcac gccaagcgcg aggaacatgt
     1141 agccaatctg gctcatggta gagtaagcga gaacacgttt gatgtctgtc tgtaccagcg
     1201 cggcaaaacc ggccagcagc agcgtaaccg ccccgacaat acccaccaga tgcagaactt
     1261 ccggggtcat caggaacagg ccgtgggtac gggcgatcag gtagacaccc gcggttacca
     1321 tcgttgcagc gtggatcagc gcggagacag gcgtcgggcc agccatcgca tcggcaagcc
     1381 atgtctgcaa cggcaactgc gcagatttac cgaccgcacc gcccagcagc atcagcgtcg
     1441 cccacatcag catgttattg ccgtcagcaa agtgcgctgg tgccagttcc accatttcgc
     1501 ggaagttcag ggtgcccagt tcgttgtaaa gaatgaacag tgcgaaagcg aggaacacgt
     1561 cacccacacg ggtcacgacg aacgctttca ttgccgctgc gccattcttc ggatcggtgt
     1621 aatagaaacc gatcagcaga taggagcaca ggcccacgcc ttcccagccg aggtacatca
     1681 gcagcaggtt gtcggcaagc accagaacta ccatgctggc gatgaacagg ttggtgtagg
     1741 cgaagaagcg agagtagccc tcttcaccgc gcatgtacca ggaggcgtac atgtgaataa
     1801 ggaaacccac accagtgacc accgagagca tggtcagcga caggccgtcc agcaccagat
     1861 tgaaaccgat gttaaagtcg cctaccgaca tccacgtcca cagcggctgg ctgtatgact
     1921 gctcgccgtt agcgaagaaa tcaacgccga taaaggcggt taccagcgcc gccaggccca
     1981 cagagccaac gccgacgatc gccgaaacgt tttcagacca gcgcccacgg gagaacgcca
     2041 gcaggacgaa gccaatcaat ggcaaaataa tggttaaggc aagcatgttc atccgcgcat
     2101 ctcacttact gaatcgatgt tcaggttctg gcgacgacgg tgaagttgca gcagcagcgc
     2161 aaggccgata ctcgcttctg ccgccgcgag gctgatggcg agaatgtaca tcacctgacc
     2221 gtcggtctga ccccagtagc ttccggcgac cacaaaggcc agcgcggagg cgttaatcat
     2281 gatttccaga ccaatcagca taaacagcag attgcgacgg ataaccagac cggttaagcc
     2341 aagaacgaat aagattgccg cgaggatcag tccatgttgt aaggggatca tgcgtgctcc
     2401 tccgtttttc ttttcgcgct gtcgtcttta cgattgctca gcacttcacc cgcacgctct
     2461 tcacgaccga cgtggaaggc cacaaccaga cccgcgagca gcagcataga agccagttcc
     2521 accgccagta cgtaaggccc gaacagcgta atacccactg ctttagcact gattggcgta
     2581 ccgtcgatac cctgatcatt aacaccaagg atggcgtaaa caatcaccac cagcatgatg
     2641 gccgacaaaa ttgccggacc aatccacacc tgcggtttca gccactggcg ttcctgttcg
     2701 atttctgaac cgcccaggtt gagcatcatc accacgaaca cgaacagcac cataatggca
     2761 cccgcgtaga cgataatttc cagcgcaccg gcgaagtaag cgcccagtga gaagaacacc
     2821 ccggaaatcg ccagcagcga aataatcagg tacagcagtg cgtgtaccgg attggtatgg
     2881 gtgatcactc gcaaggtcgc aagtatggct atcaggccac agatataaaa agcgaactcc
     2941 attgcccctc tccttacggt aacaggctct tgacgtcgat aggcttggct tcgttctctg
     3001 cttcgccctt atctttgccg tcgattgcca tacctgccat ccggtagaag ttatattccg
     3061 ggtatttgcc cggaccggag atcagcagat cctctttttc gtaaaccaga tcctggcgct
     3121 tgtattcccc catttcgaaa tccggggtta actgaatcgc cgtggtcgga caggcttctt
     3181 cgcacagacc acagaaaatg cagcgtgaga agttgatgcg gaaaaattcc gggtaccagc
     3241 gaccgtcttt ggtttctgct ttttgcagcg agatacagcc gaccgggcag gctaccgcgc
     3301 agaggttaca ggctacgcaa cgctcttcgc cgtccgggtc gcgggtcaga acgatacgac
     3361 cacgataacg gggcggcaga tagaccggct cttccgggta cattcgcgtt tcgcgtttgg
     3421 cgaacgcgtg caggccgatc atccagatac tacgaacctg ggtgccgaaa cctactaaca
     3481 attcttttaa ggtcatggtc ttattgcccc ttattgcgcc tgccagagaa tgacagccgc
     3541 cgttaccagc aagttgatca gcgtcagcgg caggcagatt ttccagccga aggacattac
     3601 ctggtcataa cgcggacgcg gtaacgacgc acgaatcaaa atgaacatca tcataaagaa
     3661 cgcggttttc agcgcgaacc agatgaatgg cggtaacaac gggccttgcc agccaccgaa
     3721 gaacagcgtc accatcaatg cagagatggt cacaatcccg atgtactcac ccacgaagaa
     3781 cagaccgaac ttcataccgg aatattcaat gtggtaacca tccgccagtt cctgctcggc
     3841 ttccggctgg tcaaacgggt gacggtgaca taccgccacg cccgcgatgg caaaggtaat
     3901 aaaaccaaag aattgcggga taacgttcca cacatgcgcc tggctgttga cgatgtcggt
     3961 catgttgaat gaaccggcct gcgccaccac gcccatcagg gaaagcccga ggaacacttc
     4021 gtagctcagg gtctgcgcag aagcacgcat cgcacccagc aacgagtatt tgttgttact
     4081 tgaccagccc gcaaacagca ccgcgtaaac cgccagacct gccatcatca ggaagaacaa
     4141 aatcccgatg ttcaggtcgg caaccaccca acccggactg actggcacaa tcgcaaaggc
     4201 cagcagcagc gaggtaaagg caatcatcgg tgccagggta aagatgacgc gatccgagaa
     4261 tttcgggatc cagtcttctt taaagaacat tttgatcatg tccgcaacca gctggagcga
     4321 accgccccag ccaacacggt taggtccgta acggttctgg aacagaccca gcaggcgacg
     4381 ttcgccaaag ctcatgaatg ccccgcaggt gacaaccacc agcaggatca ccaccgcttt
     4441 gaggatggtc agcaggatct caatcagttc cggtgatatc caactcattg ttgtgcctcc
     4501 ttgagatcct caagatgcgc gccagccagc accggagcaa taccggacat acccatcggc
     4561 aagcccacct gccctgccgt cagcccttca gcgatttcaa ccggcagcgt gaccgtgttg
     4621 ccatcgtaac taaaggagac gtgtgtacct gcgttcacac ctaacttcgc ggcatccgct
     4681 gggttgagtt tgatgtacgg ctgcggcata cggctctgga agaccggagc acgctgtgac
     4741 aattcatcgc tgccaaacag gtggtaatac ggcgcgatac gccatttccc gtcctgcggc
     4801 tggaagcgtg ccggtacgct ggtgaagtaa tccagaccat tttcgctggt ttcaaacaga
     4861 cgcacgcccg gatcgccaaa gcgcagtttg ccgcccactt cgtcctggaa tttgttccac
     4921 gcctgcgggg agttccagcc cggcgcccag gcaaacggca cttgcgaacg gtgcgcagtc
     4981 ggctggttgt taccttccat cgagaaggtg aacatggtgt caatatcctg cggctgacgc
     5041 ggctcatgaa cgctgatatt ggcgcgcatg gcggtacgac cgctgtaacg gtgcggttca
     5101 cgggccagtt cctgcccacg aatacggaat gtcgcatccg gcgcagcatc tttgatacct
     5161 gccagttccg ggattttcgc cacaacagcg tcaatcacat ggtcgagctg cgtccagtcc
     5221 acttcacggc tcagcagggt gctgtgcagc gagtgtaacc agcgccagct ttccagcatg
     5281 acagttttgc tgtcgtaata agcaggatcg taaacctgga agaaacgttg ggcgcggcct
     5341 tcgttgttga tcaccgtacc gtcgctttca gcaaagctgg cagcagaaag taccagatgg
     5401 gcgttttcca taatcgctgt gcgttgatga tcaaccacca tcaccagcgg cgctttagcc
     5461 agcgcagcat tcacgcgggt agcggaagcg tgacgatgca gatcgttttc cagcaccacc
     5521 accgcgtcgg cgcgtccggt ttccagttcg gtcaacgctt cttcaagcga accgccaccc
     5581 ataatgccca gccccatgct gttgacggaa cgggcaatca tggtgatacc aacgtcagcg
     5641 ccgcgacctt tcagggcttt cgcgacgtta gccgcagcct gaatcacctc tatgctaccg
     5701 gcgttcgtcc cggagataat caacggtttc ttcgcacctg ccagtgcctg cacgatgacg
     5761 tcgattttgc tttgcagctc aggttcgata ccgtcaaccg ctggcgcaga gttatccagc
     5821 gcatgggcga tggcaaaacc taaacgcgcc tgatcttcaa ccggtgcgcg gtaagtccac
     5881 gccgcgatat catccagacg ggtgtcatca acgttggtaa caaacagcgg atgcttcgca
     5941 cgttgaccga tgttgaggat tgccgcaatc tgccagtcag ccactttctg tgctgccgcc
     6001 atttcgcgcg ctttaccttt cacagcctga cgcactgcca gcgcgacgcg cgcgccggtc
     6061 tgggtaacgt cttcgcccag caccagtacc gcatcgtaag attcgatttc gcgcagagcc
     6121 ggagtataaa tgccgccttc acgcagcact ttcagcgcca gttgcagatg ttcctgctca
     6181 ccgtgagcga taccggtgta gaagttttct tcgcccacca gttcacgcag cgcaaagttg
     6241 ctttccacgc tggcacgcgg agaaccaata ccgatcactt tcttcgactg acgcagaata
     6301 tctgccgcgc cctgcattgc ctgttcggcg ttgagggtaa tgaaatcatc gccacgacgc
     6361 tgtactggtt gacgcggacg atctttcagg ttgacgtaac cgtaaccgaa acgaccacgg
     6421 tcgcagagga agtagtggtt taccgtaccg ttgtaacggt tttcgatacg acgcagttcg
     6481 ccgtaacgtt caccggggct gatgttacag ccgatggaac attgctggca gatgctcggc
     6541 gcaaactgca tatcccattt acggttgtaa cgctcggagt gcgttttgtc ggtaaatacg
     6601 ccggtcgggc aaatttcgac caggttaccg gaaaattcgc tttccagcgt gccgtcttcc
     6661 gggcgaccga agtagacgtt gtcgtgcgca ccgtaaacgc ccagatccgt accgtcagcg
     6721 taatctttgt agtaacgcac gcagcggtag caggcgatgc agcggttcat ttcgtgagag
     6781 atgaatggcc ccaaatcctg attacggtgg gtacgtttgg tgaaacggta gcgacggaag
     6841 ctgtgtccgg tcatcacggt catatcctga agatggcagt taccgccttc ttcacatacc
     6901 ggacagtcgt gcgggtggtt ggtcattaac cactcgacca cactttcacg gaactgtttc
     6961 gcttcttcgt cgtcaatgga aataaaggtg ccatcggaag ccggtgtcat acaggacatc
     7021 accaggcgac cacgcgtgtc ttccgcgttt tggtattgct tcaccgcaca ctggcggcaa
     7081 gcaccgacgc ttcccagcgc cggatgccag caaaagtaag gaatatcaag gcccagagac
     7141 agacaagctt ccagcaggtt gtccgctccg ttgacctcgt attctttgcc gtctacatga
     7201 attgtagcca ttagcatgct tccagttttc tcggtcaggg accgagcgtt aatcgaaatt
     7261 cggttaccag cgctctttca gcaggttcgg ctgaatccca ttaatcaaat gggtattgct
     7321 gaacggctgt ttgattcccg cctcaaattc ttcgcggaaa tatttgatgg cgctctgtaa
     7381 cggctccact gcaccaggtg cgtgggcaca gaaagtttta cccgggccta agaatcgaca
     7441 cagttgctca agtgtttcga tatcgcccgg ctgaccttca ccacgctcca gcgcacgcag
     7501 aattttcacg ctccacggca gaccgtcgcg gcacggcgta caccagccgc aggactcacg
     7561 ggcgaaaaac tcttccaggt tacgcaccag cgacaccatg ttgatctcat ggtcaaccgc
     7621 catcgccagc gccgtaccca gacggcttcc cgctttaccg atactttcga attccatcgg
     7681 caggtcaagg tgcgcttcgg tcaggaagtc ggtgcccgcg ccgcctggct gccaggcttt
     7741 gaatttcaga ccgtcacgca taccaccggc gtaatcttcg aggatctcgc gcgcggtagt
     7801 accaaacggc agttcccaca gtcccggatt tttcacccga ccggagaagc ccatcagctt
     7861 ggtgccagca tctttacttt tcgagatgtt ctgataccac tccacgccgt tagcgaggat
     7921 cgccggaacg ttacacaggg tttcgacgtt gttgacacag gtcggtttgc cccatacgcc
     7981 ggaggttgcc gggaaggggg gtttcgagcg tgggttagca cgacgccctt ccagggagtt
     8041 gattaacgct gtttcttccc cgcagatgta gcgccctgcc ccggtatgga cgaacagttc
     8101 gaagtcgaaa cctgttccca taatgttttt gccaagcaag cccgcttcag tggcttcggc
     8161 aatggcgcgg cgcagattta ctgccgcttc gatatattcg ccacgcagga agatgtagcc
     8221 acggtaagct ttcagcgcaa acgcggagat gagcatacct tccaccagca ggtgcggcag
     8281 ttgctccatc agcaggcggt ctttataggt gcctggctcc atttcatcgg cgttacacag
     8341 caggtaacgg atgttcatgg attcgtcttt cggcatcagg ctccatttca ggccggtaga
     8401 aaagcccgcg ccaccgcgcc ctttcagacc agcgtctttt acctgattaa cgatttcgtc
     8461 cggagacagc ccggtcagcg ccttacgcgc gccttcgtaa ccgtttttgc tgcgatattc
     8521 gtccagccac actggctgtt tgtcatcgcg cagacgccag gtcagcggat gcgtttcggg
     8581 agtacggata atgtttttca tttataccgc tccagcagtt cagggatcgc ttccggggtc
     8641 agatgcgcgt gagtatcctc atcgatcatc atgtttggcc ctttatcaca gttccccagg
     8701 cagcaagttg gcagcagcgt aaagcgaccg tcaaatgtcg tttgccctgg tttgatgttt
     8761 agctttttct cgagcgccgc ctgaataccc tggtaaccgt tgatatgaca gaccacgctg
     8821 tcgcaataac ggatcacatg gcggccaacc ggctggcgga agatctggct gtagaacgtt
     8881 gccacacctt cgacgtcgct tgccggaata cccagcacat cggcgatcgc gtggatcgca
     8941 ccatccggca cccagccacg ctgcttctga acgattttca gcgcttcaat ggacgccgca
     9001 cgcgggtctt cgtagtggtg catctcgtgc tcaatcgctt cacgctctgc cgcactcagc
     9061 tcaaaagcct cggtttgtgg ttgttgattc tcgtgcataa ttagcggtcc acatctgaca
     9121 taacaaaatc gatactgccc agataaacaa tcaggtcaga caccaggctg ccgcggatcg
     9181 ccgccggaat ttgctgcaaa tgcgcataac tcggcgtgcg gatacgagtg cgataactca
     9241 tggtgctacc gtcgctggtc aggtagtaac tgttgatccc cttggtcgcc tcaatcatct
     9301 ggaaagattc attggcaggc atcaccggcc cccacgacac ttgcaggaag tgggtgatca
     9361 gggtttcgat atgttgcagc gtgcgctctt tcggcggcgg cgtggtcagc gggtgatccg
     9421 ctttgaacgg gccttccggc atgttgttga ggcactgctc aagaatgcgc agactctggc
     9481 gcagctcttc cactttcagc attacgcggg tgtagcagtc agaaacacca ccaccaaccg
     9541 ggatttcaaa gtcgaagttt tcatagccag aataaggacg cgccttacgc acgtcgaagt
     9601 cgatcccggt agcacgcagg cccgcgccag tggtgcccca ctccagcgcc tctttcgcgc
     9661 cataggcggc aacgccctgg gaacgacctt tcagaatggt attttgcagc gccgctttct
     9721 cgtaagacgc cagacgtttc ggcatccagt cgaggaactc acgcagcagg cgatcccagc
     9781 cgcgcggcag gtcgtgcgct acgccgccaa tacggaacca cgccgggtgc atacggaaac
     9841 ccgtgatcgc ttccaccaga tcgtaaattt tctgacgatc ggtaaaggcg aagaacacgg
     9901 gcgtcattgc gccgacgtcc tgaataaagg tcgagatgta cagcaggtgg ctgttgatac
     9961 ggaacagttc ggagagcata acgcgaatga cgttaacgcg atccggcacg gtgatcccgg
    10021 ccagtttctc taccgccagc acgtaaggca tttcgttaac gcagccgccg aggtattcaa
    10081 tacggtcggt atacggaatg tagctgtgcc aggactggcg ctcgcccatt ttctccgcac
    10141 cacggtggtg gtagccgatg tctggtacgc agtcgacaat ctcttcgccg tcgagttgta
    10201 aaacgatacg gaaagccccg tgcgccgacg ggtggttcgg accgaggttg aggaacatga
    10261 agtcctcgtt ttcggtgccg cgcttcatcc cccactcttc cggtttgaag gtcagggctt
    10321 ccatctccag atcctgtttg gctttggtca gctcaaacgg cgagaattcg gtagcgcgcg
    10381 ccgggtaatc tttacgcagc gggtgacctt tccaggtttg cggcatcatg atgcggcgca
    10441 ggttcgggtg accgtcgaaa gtaatgccaa acagatccca ggtttcacgc tcataccagt
    10501 tagcgttcgg gaacagtttg gtgaaggtcg gtacgtgcag gtcgttttct gccagcgcca
    10561 ccttcagcat gatgtcgcgg ttacgatcga tagaaatcag atggtagaaa acggaaaaat
    10621 ccgcggcagg taacccttcg cggtgtgtgc gcagacgttc gtccatgccg tgtaagtcaa
    10681 acagcatgac gtaaggtttc ggcagtttct ttaagaaatc gccaacttcc agtaattgtt
    10741 cacgcttgat ccacacaacg ggaaccccgg tgcgagtcgc ctgaacagta aaggcatccg
    10801 gcccaaaacg gttgcgcagt tcgccaatca ccggatcatc aagatgatcg cgggtctgcc
    10861 aggcgggttc ttgcgcggtt aagtcggtca tattgttcac cattgcaaat ggtccgtggt
    10921 gactgtcggc ggagtaagct tcgcgaaata gatttaggaa taagcgaaga aaaatccctt
    10981 tgccgacagg cgcaaattaa atctcgtcag gtgtacgcag gttggttacg gcaatgcgtt
    11041 caccgcgctt gcgttcgcgc tctgattgca tattggcgcg ataaacgccc tgatcgccaa
    11101 ccacccagga gagcggacga cgttctttgc cgatagattc ctgcaacagc atcagtgcct
    11161 gcatgtacgc ttcaggacgc ggcgggcagc ccgggatata cacatcaacc gggatgaatt
    11221 tatcgacgcc ctgcacaacg gaataaatat cgtacatacc gccggagttg gcacaggcac
    11281 ccattgagat aacccatttt ggttccagca tctggtcata cagacgctga ataaccggtg
    11341 ccattttggt aaagcaggtt cctgcaacca ccatcaggtc agcctgacgc ggcgaagcac
    11401 gcaatacttc tgcgccaaaa cgcgccacgt catgcaccgc ggtaaacgaa gtcaccatct
    11461 caacgtaaca gcaggaaaga ccgaagttat acggccaaat tgagttttta cgaccccagt
    11521 taaccatgtc attgagcttg cccataaaca cgtttttgtt aacttcttgc tccagagggt
    11581 cggttacgat ctcctgcttt tgcagggggt aacggtcgtt ctcaccgttg ggatctatgc
    11641 gggtgagcgt ataatccatc ttaatgcctc gcggttagcg ttgacgatta gcgatactgt
    11701 tcgtttccgg gttcatacgc tcgcggcgtg aacgcgcggg cgtccagtcc agcgcgccaa
    11761 tacgcaccag ataaaccaga cctgccagta acacaaaaat aaaaattgca gcttccacaa
    11821 agcctaccca gccgctttcg cggatagagg ttgaccatgc gaacagatac agcgcttcaa
    11881 cgtcgaagat aacgaagaac atggccacca gataaaactt ggcggacagg cgtaagcggg
    11941 cggagccgac cgagtcgata ccggattcaa acggcacgtt tttcgacctc gcgcgtgcgc
    12001 gaccgcctaa aaaccaaccg cctaccagca tcaggcaaca caggccaatg gcaacgataa
    12061 gaaagatagc gaatgcccag tgatgagcga tgacttcagt ggatgttgac atactcattg
    12121 cttactcatc aaaagtagcg ccagattcac tgctcttcac ggcagatgga cgccacatcg
    12181 attcatgggg aggaataaaa aaaaccttac aatcactgta gaaattcttt tatacagcta
    12241 attgatgtgg tcttttactc ctttctataa ccttttgtca actttaacaa aagtttcttc
    12301 acattagttt acataatatc aacaccatta gcatttaatg ccctttcacc ccagatcctt
    12361 gacgactcca ggataattag atgttgttga atcgtgtccg ttgtgaagca atggaaaaaa
    12421 tacgggtcta ttttgacagg aatttgtgtc gattcctccc cccaaaagag agtattttct
    12481 tgatctgtga cacgcttttg tcattccata acaaaaacgc agcaacaaat ttacgtattt
    12541 tttaacatca ttgtagcagg tgattttttt caggcgatta tttgtgcgtt cgggacgtga
    12601 atctctggtg attgaaaaat gaacagtttt gtacgttctg cactatgcga ggaaggattt
    12661 ttactaaaaa aaagccgctg gggtttaaaa cacccccagc ggctcgtttt ttacactatt
    12721 gtctcaggaa ttatctatcg tccgtcgatt actcgatatc cctttcaatc aacaatgaat
    12781 catccccttc cggagcagac attgggctgt attgccacgg attatggtag ctctccatcg
    12841 cctgatagat cacctgggcc agctcattat tactggacgg gtcatagcac agcaaatatt
    12901 cggtatcagg tagcggcggt aagccatcta ctccactcag cacacgcagg tccgggctca
    12961 tcatctcaac cggccttgcc gtcacgccaa gaccggcttt cactgccgca cgaacggccg
    13021 gaagcgtcga ggcgacataa gccagtcgcc atggaatatc tgctttatta agcgtcgcca
    13081 gcaccatatc gcgaaacgga cttggatcat ccagcaatac aagagggatc ggctcgcctt
    13141 tttgcaatac gtattccgct gcgcagtacc agtgtgttgg cgaggtacgc aggttcaatg
    13201 ccttaaacgc tgaaggacga tgggtggtta ccatcaaatc cacttcctgg gattccagca
    13261 tttctgccat ataggcatta cgtttaacgc ggacatccag cgctaatttc ggataaaccg
    13321 aactcacgcg atttaacagg aaaggtaaga tcgtatcggc agattcatct gaagcaccga
    13381 tagttaacac gccctgaaga ttactgaaca ttaatgatga gcaggcctca tcattaaaac
    13441 gcaggatttt cctggcgtaa ccaagaagtt gaatgccatg ttcagttaac agtttgttgc
    13501 gaccgtgacg agcgaacagt tctttcccaa cgagttgttc cagacgctgc atttgctgac
    13561 ttacggcgga ctgagtacga cacacagcgg cagctgcggc agcaaaagtg ttcagatcgg
    13621 caacagcaac aaatgttctc agcagatcga ggtcgaggtt aattatcgga cgatttgcac
    13681 ttatcatata ttatcactta ctggcggctc atactgagct ggttaatgct gtgcacacac
    13741 aaacaagcaa ttccatttgt aatgtgcctc cctggcagtt tcatccggaa cccggacgaa
    13801 agtaaaaatg catatgagtt gcactaaaaa agcgactcac attgttccgt tataatgcct
    13861 gaagtagatc acagaatata tcttcaggga tcgcatatct attaagttac tcactctttt
    13921 ctatttatga catgcgcgtg tttgtataaa tgtaaatgtg agtccttgtt ccactctcgt
    13981 gcagcatcgc tggtcatacg cgaacacgta caaacagcaa tggtgaggcg gcatcaagag
    14041 cagggatccg tcatttatgc gagcatttta ccccaaaaac cttttattta taagggtcat
    14101 tgcgaattat ctgatgcaaa gttatgttat gttaggcaaa gtaatcttct attattaata
    14161 agcacatcaa aacctttttg aatattaaat aataattaat tagcatcatc ctcattcatt
    14221 aattccgctt aacaatagtt tcacaccttg cccccattac gacacctcgg aaatcatcac
    14281 gctgttaaac tctccgtaat gcggaaatat catcatcgcg tttcattagt gaattcttaa
    14341 atgaggcatt ttcacacaat tatcttacag ataaaaaaac cagacttaca attaagaatc
    14401 agaacaattc actatataac attgcatgta aagcatatac acctcattat tttgtcatta
    14461 ttaagttatt aacagcacaa tcgagccttc ccctctggca aaatcttatt ctgcagacct
    14521 tcaaaacacc gtcctggggg agtacattgt tctaagctga cttccacggc agggagtggc
    14581 gataacagca aaaaaggtca agattcatgt cccccattga aaaatccagc aaattagaga
    14641 atgtctgtta tgacatccgt ggtccggtgc tgaaagaagc aaaacgcctg gaagaagaag
    14701 gtaacaaggt actgaaactg aacatcggca acccagcccc gttcggtttt gacgcgccag
    14761 atgaaatcct cgttgacgtg atacgcaacc tgcctaccgc tcaagggtat tgcgattcca
    14821 aaggtcttta ctccgcgcgt aaagccatca tgcagcacta ccaggctcgt ggcatgcgtg
    14881 atgttaccgt ggaagatatt tacatcggca atggtgtatc ggagcttatc gttcaggcaa
    14941 tgcaggcatt gctgaacagc ggggacgaaa tgttggttcc tgcaccagat tacccactat
    15001 ggaccgcggc ggtttcgctt tccagcggta aagcggtgca ttatctttgc gatgaatcct
    15061 ctgactggtt cccggacctc gatgatattc gcgctaaaat tacgcctcgt acgcgtggga
    15121 tcgttattat caacccaaat aacccaaccg gcgcggtata ttccaaagag cttttaatgg
    15181 agattgtgga gattgcacgt cagcataatc tcattatctt cgccgatgaa atttatgaca
    15241 aaattcttta cgacgacgct gagcatcact caattgcgcc gctggcacct gacctgctga
    15301 ccattacctt taacggactg tcgaaaacgt accgcgttgc aggcttccgt caggggtgga
    15361 tggtgttgaa cgggccgaaa aaacacgcca aaggctacat cgaaggtctg gaaatgctgg
    15421 cttcaatgcg cctgtgtgct aacgttcctg cgcaacacgc cattcagacc gcgctgggtg
    15481 gttatcagag catcagtgaa tttattaccc ctggcggtcg tctttatgag cagcgtaacc
    15541 gcgcgtggga actgatcaac gatattccgg gcgtttcctg cgtgaaacct cgtggtgcgc
    15601 tgtatatgtt cccgaaaatc gacgccaaac gctttaacat tcacgacgat cagaaaatgg
    15661 tgctggattt cctgttgcag gaaaaagttc tgttggtgca agggacggca ttcaactggc
    15721 cgtggccgga tcacttccgc attgtcacgc taccgcgtgt cgatgatatc gagctgtctt
    15781 tgagcaagtt cgcgcgtttc ctttctggtt atcatcagct gtaatcttaa tttcactgcc
    15841 ggagattgca tccggcagcg ttatcccgcc acaatgacct gatgatgtca tcatacgtaa
    15901 ggtcactatg aaacagagcc atttttttgc ccatctctcc cgcctgaaac tcattaaccg
    15961 ctggccgctc atgcgcaacg tgcggacgga aaatgtgtcc gaacacagtt tgcaggtagc
    16021 gatggtcgcc catgcgctgg caactatcaa aaatcggaaa tttggcggta atgtcaacgc
    16081 cgaacgtatc gctttactgg cgatgtacca cgatgcctca gaagtgctca ccggcgatct
    16141 ccctactccg gtgaaatact tcaattcgca aatcgctcag gagtacaagg ctattgaaaa
    16201 aatcgctcag caaaaactgg tcgatatggt tccggaagag ctgcgggata tctttgcgcc
    16261 gttaattgac gagcatgcat atagcgatga agaaaaatcg ctggtgaaac aggcagatgc
    16321 actgtgtgca tatctgaaat gtctggaaga actcgcggcc ggaaataatg aattcttgct
    16381 ggcaaaaacg cgactggaag cgacgcttga agcgcgtcgc agccaggaga tggactactt
    16441 catggaagta tttgttccca gcttccatct ttcgctcgat gagattagcc aggattcacc
    16501 gctgtaagca gccggagtct gcgtcgcatc aggcaataag cgccggatgc gacatcaggc
    16561 tcttgtcaaa acggaaacag catcgggatc atcaccacac aaaccgccat cacgataatg
    16621 gtgaacggta cccccaactt cacaaagtca ctgaagctgt aatttcccgg acctaaaacc
    16681 agtgtgttaa caggtgaaga aaccggtgtc ataaaggcgg cggatgctgc catcgccacg
    16741 accatcgcga atggataagg cgacaccccc atcgttttgg cagcagccag cgcaatcgga
    16801 gccatcaaca ccgccgtcgc ggtattagag ataaatagcc caataaccgc cgacaagaca
    16861 aacaaacaac ccagcatcat atgtggcccg taaccaccgc caatgtccat cagccctttc
    16921 accgccagcg cgacacctcc cgttttctgt aatgccacag caaacggcat catcccaacg
    16981 atcaaaataa tgctcggcca gtgaatggat ttataggcgc tttcagcatc tatacagcgg
    17041 aatttcccca tcagcaggca ggcgatgata gcggcaacag gattaggaat ttcatctgtc
    17101 agcattaacg ccaccattag caccagacag aaaatggcat ggggtgcctg gctgtgcgcg
    17161 ggcgatgctt cactcacctc ttccggtaag ttcagcgcta cgaagtcgcg gccctgtttg
    17221 gccagcatac cgatcagttt ccagttaccc acaaccagga tgatatcgcc cagcagcaga
    17281 ggctcatccg ccagcgaacc ttccagcgcc acgccattgc gctttagccc caccacattc
    17341 agtccgtagc gggtacgaaa accaatttcg cgcaccgatt taccaatcag ttctgactca
    17401 ggaattaatg aaatctctgc catgcccaca tcaagggcct ggtcagaaaa atactcgccg
    17461 cgcagtacca tcggctccag caattgctca ctacaaaatt gccggagatc gacatcagcc
    17521 gcagacatat caataagcaa aacgtcacgc gcgcgaaatt cagaaacccc attaacgttc
    17581 acgataacgc gacgaaaacg ccgccagcgt tcaacaccga tgacgttagc gccataacgc
    17641 tcacgtaatt tgagatcatc cagccgttga cctatcattg gcgatccggg gcgaatagcc
    17701 agacgtcgcg cacgcccggt cagtcgatat tcacggataa gatcgcgaaa ggttcgacgc
    17761 gtccagcctt cgcgctgcgg ggtctgggta tcccctttca gcatgaaacg catcactaac
    17821 atatacaaaa tacccagcac cagcacgacc aggccaatag gtgttacgct aaagaaactg
    17881 aagccgtgat agccttcacg cagcaattca ctgttgacta ccaggttcgg cggcgtcgcc
    17941 accagcgtca tcatgccgct tatcagcccg gcaaaactca acggcatcat cagacgtgac
    18001 ggcgaagttt gcatacgcat ggcaacgctt aacaccacgg gaataaagat agcgacaacg
    18061 ccggttgaac tcataaacgc gccaagcccc gcgacggtca gcatcaacaa aaccaacatt
    18121 ttgatttcac tgttgcccgc aactttgacc agccatgttc ccattacggt ggcaacaccg
    18181 gtacggacca aaccatcgcc aataataaac aaggcggcaa tcaggacaac gttaggatca
    18241 gaaaagccgg aaaatacttc ggggactgtc agcgttccgc ttaatgcaaa cgcgacaata
    18301 acaaacaaag cgaccgcatc catacgcact ctgcccgtcg caaacaagac aatggcaacg
    18361 gccagtaatg aaagaaccca aatcaattca ccgttcacaa cttatccttg ttaattgagg
    18421 gagatgtctt gattctgcca taaaaaagcc ccgacgagac ggggctaaat catgatcagg
    18481 tgtttcactg aataataaca tcgccatttg gctgtttggt cacagtaatt tgctccagac
    18541 tgtggaggac caaatcgacc tcattcaggc gcggggtatc tgccggagcg ttaaccgcaa
    18601 tgacatggca acccgccgcc aggccagaaa gcacgccagc gggagcatct tccaccacca
    18661 cacactcctg cggcgcaagc cccagcagct gcgcacctaa cagatacgca tcaggttctg
    18721 gttttccgcg cttcactcgc tcagcggtta caaacacctc tggtgcggga agcccagcta
    18781 ttttatggcg cgctcgcgct accggcatgg agccagaagt cacaatggcc cacggaatac
    18841 ctgctttatt caaatgactg agtaaggcga ttgcccccgg aagcgcggta ataccttcgg
    18901 tttccgtggc ctcgatttgc tccagacgcg taaactcggc ggcaatatca gcctcggatt
    18961 tgcccgccat aaaatggcgc agagaggtga tcgcctgttt accgtgaatg aaagccagca
    19021 cctcttccgg cgctaaccca tgacgtctgg cccagttgct ccacgcccgt tctaccgcag
    19081 gcagggaatc caccagcgtt ccatcaagat caaacagaaa acctttgcac cgcacgcggg
    19141 cctcctcagg cgttgataat ttgattaatt tcgttggcgc tcaaatggta ctgacgcggg
    19201 caggcatgcc acacattaag catgcgctga tatttttccc acattggcgt ctgggcgtta
    19261 aaaccgtgag ttccggcgtc aaaatgggta tagcgccctt ccacattaac cataaagcgg
    19321 acataaccga ggtaacgtgc ttcagtggcg gcgtcaaagc cgaggaaggt gacacgacgt
    19381 tcatcgatgg attgctggtc ctgcaaatta gaccaggaaa catgcaacgc atgatacatc
    19441 tccataatgt cgatgatagt gcggcaggtt tcttctttca gctcgccaaa ctcgcgatcc
    19501 aattcacgca tctgtaatcc gtaaccacgc tcaataattg tttgcaggcg acggtaacgt
    19561 tcagcatttg ccggatcgag catagtcatc atcttgtact gattagacaa aataagacgt
    19621 tgcgcgttgg tcatttccat tgttgactcc tgtatcacta tactacggtg aaaaaaaaga
    19681 aggcttagta tgccttcttt tatatgcgta atcaggggtc aatta
//
LOCUS       DAMZET010000077        19507 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.077, whole genome shotgun sequence.
ACCESSION   DAMZET010000077 DAMZET010000000
VERSION     DAMZET010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.077"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            198..1295
                     /gene="mltA"
                     /locus_tag="POB34_002743"
     CDS             198..1295
                     /gene="mltA"
                     /locus_tag="POB34_002743"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HDI5919653.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1534..2340
                     /gene="tcdA"
                     /locus_tag="POB34_002744"
     CDS             1534..2340
                     /gene="tcdA"
                     /locus_tag="POB34_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HDI5919654.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAKVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2391..2834)
                     /gene="csdE"
                     /locus_tag="POB34_002745"
     CDS             complement(2391..2834)
                     /gene="csdE"
                     /locus_tag="POB34_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HDI5919655.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2834..4039)
                     /gene="csdA"
                     /locus_tag="POB34_002746"
     CDS             complement(2834..4039)
                     /gene="csdA"
                     /locus_tag="POB34_002746"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HDI5919656.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQLGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4231..4458
                     /locus_tag="POB34_002747"
     CDS             4231..4458
                     /locus_tag="POB34_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HDI5919657.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4809..5726
                     /gene="gcvA"
                     /locus_tag="POB34_002748"
     CDS             4809..5726
                     /gene="gcvA"
                     /locus_tag="POB34_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HDI5919658.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5745..6140
                     /locus_tag="POB34_002749"
     CDS             5745..6140
                     /locus_tag="POB34_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDI5919659.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6133..7233
                     /gene="rlmM"
                     /locus_tag="POB34_002750"
     CDS             6133..7233
                     /gene="rlmM"
                     /locus_tag="POB34_002750"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HDI5919660.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7277..8008)
                     /gene="fucR"
                     /locus_tag="POB34_002751"
     CDS             complement(7277..8008)
                     /gene="fucR"
                     /locus_tag="POB34_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HDI5919661.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8066..8488)
                     /gene="fucU"
                     /locus_tag="POB34_002752"
     CDS             complement(8066..8488)
                     /gene="fucU"
                     /locus_tag="POB34_002752"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HDI5919662.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8490..9908)
                     /gene="fucK"
                     /locus_tag="POB34_002753"
     CDS             complement(8490..9908)
                     /gene="fucK"
                     /locus_tag="POB34_002753"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HDI5919663.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQLLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(10017..11792)
                     /gene="fucI"
                     /locus_tag="POB34_002754"
     CDS             complement(10017..11792)
                     /gene="fucI"
                     /locus_tag="POB34_002754"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HDI5919664.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQKEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11825..13141)
                     /gene="fucP"
                     /locus_tag="POB34_002755"
     CDS             complement(11825..13141)
                     /gene="fucP"
                     /locus_tag="POB34_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HDI5919665.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13688..14335
                     /gene="fucA"
                     /locus_tag="POB34_002756"
     CDS             13688..14335
                     /gene="fucA"
                     /locus_tag="POB34_002756"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDI5919666.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14363..15511
                     /gene="fucO"
                     /locus_tag="POB34_002757"
     CDS             14363..15511
                     /gene="fucO"
                     /locus_tag="POB34_002757"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDI5919667.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15566..16321)
                     /gene="xni"
                     /locus_tag="POB34_002758"
     CDS             complement(15566..16321)
                     /gene="xni"
                     /locus_tag="POB34_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HDI5919668.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVVGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16433..17800)
                     /gene="sdaB"
                     /locus_tag="POB34_002759"
     CDS             complement(16433..17800)
                     /gene="sdaB"
                     /locus_tag="POB34_002759"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HDI5919669.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVIYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17858..19147)
                     /gene="sdaC"
                     /locus_tag="POB34_002760"
     CDS             complement(17858..19147)
                     /gene="sdaC"
                     /locus_tag="POB34_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HDI5919670.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         4792 a         4817 c         5152 g         4746 t
ORIGIN      
        1 gcactatact ccgctcgcgt tttcatgcaa cctttttttt accccgatca cggatttggc
       61 atgattttga acaaaaacaa accactaaag cctatttttt gtgcaaaatt tgcgtcaggg
      121 agaggctgct catttcatta aacgcggcgg tttgttatct tcgttgcgcc ttatttttta
      181 acctgaagaa gagaacaatg aaaggacgtt gggtaaagta ccttcttatg ggcacggttg
      241 tggcaatgct tgccgcctgc tcttccaaac caaccgatcg cggacagcaa tataaagacg
      301 ggaaatttac ccagcctttc tctctggtga accagccaga tgccgttggc gcgccgatta
      361 acgccggtga ttttgccgag caaattaacc atatccgtaa ttcgtcaccg cgtctgtatg
      421 gcaaccagag taatgtttat aacgcggtgc aagagtggct gcgcgcaggc ggtgataccc
      481 gcaatatgcg tcagttcggc attgatgcct ggcagatgga aggtgccgac aactatggta
      541 acgtgcagtt tacaggctat tacacgccgg taattcaggc gcgccatacc cgccagggcg
      601 agttccagta tccgatttac cgtatgccgc caaaacgtgg tcgtctgccg tctcgtgcgg
      661 agatctacgc aggggcgctg agtgataaat atattctcgc ttacagcaac tccctgatgg
      721 ataacttcat tatggatgtg cagggtagcg ggtatatcga ctttggtgat ggcagtccgc
      781 ttaacttttt cagctatgca gggaaaaacg gtcatgccta tcgcagcatt ggtaaggtgc
      841 tgatcgaccg tggcgaagtg aaaaaagaag atatgtcgat gcaggcgatt cgtcattggg
      901 gcgaaacaca cagtgaagcc gaggttcgcg agctgctgga acagaacccg tctttcgtct
      961 tctttaaacc gcaatctttt gcaccggtga aaggggcaag tgcggtgccg ctggttggcc
     1021 gtgcttctgt tgcctccgat cgttcaatta ttccgccagg tactaccttg ctggcagaag
     1081 tgccgctgct ggataataac ggcaaattta acggtcagta cgaattgcgt ctgatggtgg
     1141 cgttggatgt cgggggcgca atcaaaggcc aacacttcga tatctatcaa gggatcgggc
     1201 cggaagccgg acaccgcgca ggttggtaca accactatgg acgtgtctgg gtgctgaaaa
     1261 ccgccccggg cgcaggtaac gtctttagcg gctgatgtga tattctgtat gccgttttta
     1321 cggatatgac agggtgaggg gtaacctcac atcctgatta ttgactaagt gcgcgttgtt
     1381 catgccggat gcggcgtgaa cgccttatcc ggcccacaaa tcatgcaaat ccaaaatatt
     1441 gcaggagtta tgtaggcctg ataagcgtag cgcatcaggc atttatgcgt ttagggtgag
     1501 ggaaaacctc acccttttta attctgaggt gctatgtctg tggtaattag tgatgcatgg
     1561 cgtcagcgtt ttggtggcac agcgcgtctg tatggtgaaa aagcgttgca actgtttgct
     1621 gacgcgcata tttgtgtggt cggtatcggt ggtgtcggtt cctgggcggc ggaagcgctg
     1681 gcgcgcacgg ggattggcgc aatcacgctt atcgatatgg atgatgtgtg cgtcaccaat
     1741 accaatcggc aaattcatgc cctgcgcgat aacgttgggc tggcaaaagc gaaagtcatg
     1801 gcggaacgta ttcgccagat taacccggag tgccgtgtaa cggtggtgga tgatttcgtg
     1861 acgccggata acgtagcgca gtatatgagt gccggttatt cgtacgtgat tgatgccatt
     1921 gatagtgtac gacccaaagc ggcgctgatt gcttattgtc ggcgcaataa aatcccgctg
     1981 gtcacgaccg gtggcgcggg tgggcagatt gacccgacgc agattcaggt taccgatctg
     2041 gcgaaaacga ttcaggaccc gctggcggcg aagttgcgcg agcgcctgaa aagcgatttt
     2101 ggcgtagtga aaaacagtaa aggtaagctc ggcgtggatt gcgtgttttc tactgaagcg
     2161 ctggtgtacc cgcagtcaga cggtacggtg tgtgcgatga aagccacggc agaagggccg
     2221 aagcggatgg attgtgcatc tggatttggc gcggcaacga tggtgaccgc cacctttggg
     2281 tttgttgcgg tttctcatgc gctgaagaag atgatggcga aagcggcgcg tcagggttaa
     2341 acaatcaggt cggatgagac gcgaacgtct tatccgaccc ggttctgtgc ttaaacctgc
     2401 ttcgtcgcag cgataatcgc ctcgcttaac gcattcaacc cctggctgcg tgaggcgcta
     2461 agctgcccac gcaatcccag ctcatcaaac aatgccagcg gcgactgtgc ctgcaattcg
     2521 gcggcggttt tcccctcaac ggcagtcaac agcaccgcca gcaggccgcg cactatacgt
     2581 ccttcgctgt cgccaaagaa gtgcatttta ccgttttcag ccacggtata tcccagccag
     2641 acgcggtttt cgcatccggc aatctcttta gcctgcgctt ttaactcgtc tggcaaagcc
     2701 ggaagctgtt tccccagcat gatcaactgg cgatatttat cttcccattg cgacaacggt
     2761 gcgaaggtat tgcgtaacgt ttctgcggtt acggttgtgc cgaacggatg tccggcgaat
     2821 tgcgggtttg tcattaatcc accaataatt ccagcgcgcg gtcaacggca ttcaccagcg
     2881 catccacatc actctttgta ttatatggcg caaaagaggc gcgcagtgtg ccggttacgc
     2941 ctaattctgc cagtagcggc tgagcgcaat gctgcccggc ccgcagggca ataccgtact
     3001 ccgccagcag cgtcaccata tcgctatgat gaacgccagc aaaatcaaag gccagcaggc
     3061 tggaatcctg gcagcggaat gaacgaaagc cgggacgttt cgccagcgca tcttccgcca
     3121 gcgttgctaa gctacggctc cagctttcgg cctggttgat atcgtaatct gccagccatt
     3181 ccagcgccgc gcttaatcct atgacaccag cgacatttgg cgttccagct tccagtttcc
     3241 acggcgcaga ttgagtcgtg aagccgtcaa aactcacttc gtgaatcatt ttgccgccac
     3301 ccagccaggg cgacatcgct tccagcagtt ctgatttgcc atacagcacg ccgatacccg
     3361 tcgggccata cagtttgtga ccagagaaag catagaaatc gatatctagt tgctgaacat
     3421 ctgcggggaa atgcaccgcc ccctgagcac catcaaccat caccaccatc ccggctgaat
     3481 gagcaaaggt aatcgctcgc gccagatccg ggcaaccgcc agtaacgttc gacatctgac
     3541 ccaacgccag aatccgacta cggggagtaa tcagttctgg caacaaatcg acatccggca
     3601 gtcgctgcgc attaagcggc aatttcacca ctttggctcc agtttgttgg gcgaccatca
     3661 gccaggggac gaggttggcg tggtgttctg ccacgctgac aataatctca tcgcccagtt
     3721 gcagacgcgg acgcgcatag cattgtgcaa ccatgttgat ggattcagtg gtgccgcgcg
     3781 tccagacgat agttttatca tccggtgcat tcagtaattg cgccactttc tctcgtgcag
     3841 cttcataacg cgcggtcagg cgttgggctt cggcaaactg gctgcgatgg acgtttccgg
     3901 cgctcaggct gtaaaactgt cgggtggctt caaccacggc ttcaggttta agcgcggtcg
     3961 cggcgctgtc gagatagacg cccgcatcct gtagagcggg aaactgggcg cgaaactgcg
     4021 cgggattaaa aacgttcatg gtactcctcg gcttgatgca ccgatcgtga cgcaaaattc
     4081 actttgatac aaggagagtg ctaaccatca gaattatctg gatatcctgg cgaagccgcg
     4141 taagtaacat atgctgaata gtgttaggcg ttaattttag cctgttaatc aatactatta
     4201 agatttatta atcgctttaa ggagaagatg atgaaaaaga ctgccgcaat tatttctgcc
     4261 tgtatgctga cttttgccct gagcgcctgt tccggttcga actatgtgat gcacaccaat
     4321 gacggacgta ccatcgtctc tgacggcaaa ccacagactg ataacgatac cggtatgatt
     4381 tcgtataaag acgctaatgg caacaaacag cagatcaacc gtactgacgt gaaagagatg
     4441 gtcgaactgg atcagtaatt cgcgatcgca aggtaaaaaa aagcaccgca attaggcggt
     4501 gctacattaa tcactatgga cagacagggt aaatgtacag gaagtgaaaa aaggtagctt
     4561 tgctaccatg gtctgaatcg cagaccaatt gcaaacacaa caacacaaca tcacaaccgt
     4621 aagccaaaag ttcaccagaa cacgcattcc gataaaactt ttcgttccgg ctcaggaagt
     4681 gccgccacta taggtatttg ctggtagaag ctcaacggac aatttataat ggcttagatt
     4741 aaaaaaacta ataggttaca cagtgtgatc taattgttaa attcatttaa catcaaagtt
     4801 taaaagccat gtctaaacga ttaccaccgc taaatgcctt acgagttttt gatgccgcag
     4861 cacgccattt aagtttcact cgcgcagcag aagagctttt tgtgacccag gccgcagtaa
     4921 gtcatcaaat caagtctctt gaggattttc tggggctaaa actgttccgc cgccgtaatc
     4981 gttcactcct gctgaccgag gaaggtcaaa gctatttcct cgatatcaaa gagatatttt
     5041 cgcaattaac cgaagcgacg cgtaaactcc aggcccgtag cgccaagggg gcgttgacgg
     5101 tcagtttact ccccagtttc gccattcatt ggttggttcc gcgactttcc agctttaatt
     5161 cagcttatcc gggaattgac gttcgaatcc aggcggttga tcgtcaggaa gataagctgg
     5221 cggatgatgt tgatgtggcg atattttatg gtcggggcaa ctggccgggg ctacgggtgg
     5281 aaaaacttta cgccgaatat ttattgccgg tgtgttcgcc gctactgctg actggcgaaa
     5341 aacccttgaa gaccccggaa gatctggcta aacatacgtt attacatgat gcttcgcgcc
     5401 gtgactggca gacatatacc cgacaattgg ggttaaatca tatcaacgtt cagcaagggc
     5461 caatttttag ccatagcgcc atggtgctgc aagcggctat tcacgggcag ggagtggcgc
     5521 tggcaaataa cgtgatggcg caatctgaaa ttgaagccgg acgtcttgtt tgcccgttta
     5581 atgatgttct ggtcagtaaa aacgcttttt atctggtttg tcatgacagc caggcagaac
     5641 tgggtaaaat agccgccttt cgccaatgga tcctggcgaa agccgctgct gaacaagaaa
     5701 aattccgctt tcgttatgaa caataattta cgtagggtac gaccatgacc agccgtttta
     5761 tgctgatttt cgccgccatt agcggcttca tttttgtggc tctgggcgct tttggcgcgc
     5821 atgtgttaag taaaaccatg ggcgccgttg agatgggctg gatccagacc ggcctcgaat
     5881 accaggcgtt tcatacgctg gcgatcttag gtctggcggt ggcaatgcag cgtcgcatca
     5941 gtatctggtt ttactggagt agcgttttcc tcgcgttagg cacggtgttg ttcagcggca
     6001 gcctttattg cctagcgctg tcccatctgc gtttgtgggc gtttgtcact ccggttggcg
     6061 gcgtgagctt cctcgcgggc tgggcgttaa tgttagttgg tgctatccgt ttaaagcgca
     6121 agggcgtaag tcatgaataa ggttgtattg ctgtgccgtc cgggctttga aaaagagtgc
     6181 gccgcagaaa ttaccgataa agccggccag cgggaaattt tcggttttgc ccgcgtgaaa
     6241 gagaatgcgg gttatgtcat ttatgaatgt tatcaacctg atgatggcga taagttaatc
     6301 cgtgagctgc cgttcagttc attaattttt gcccgccagt ggtttgtggt gggggaactc
     6361 ctgcagcatt tgccgccaga agatcgtatt acccccattg tcggcatgtt acagggcgta
     6421 gtagagaagg gcggtgaact gcgtgttgaa gttgccgata ccaacgaaag caaagagtta
     6481 ctgaaattct gccgtaaatt tactgttccg ctacgcgctg ccttgcgcga tgccggggtg
     6541 ctggcgaact atgaaacgcc gaagcgtccg gttgtgcatg tattctttat tgcaccaggc
     6601 tgctgctata ccggttactc atacagcaac aataattcgc cgttctatat gggcattccg
     6661 cgcctgaaat ttccggcaga tgcgccgagt cgttccacgc tcaaactgga agaggcattt
     6721 catgtgttta ttcctgcgga tgagtgggat gaacgcctgg cgaacgggat gtgggcggtg
     6781 gatttaggcg cttgccctgg cggctggacc taccaactgg tgaagcgcaa catgtgggtt
     6841 tattccgtcg acaacggccc gatggcgcaa agtctgatgg ataccggaca ggtgacgtgg
     6901 ctgcgggaag acggtttcaa attccgtccg acgcgcagca atatctcctg gatggtatgc
     6961 gatatggttg aaaaaccggc gaaagttgcg gcattgatgg cgcagtggct ggttaatggc
     7021 tggtgccgtg aaaccatttt caacctcaaa ctgccgatga aaaaacgcta cgaagaagtg
     7081 tcacacaatc tggcgtatat tcaggcacag cttgatgaac atggcataaa tgctcagatt
     7141 caggcgcggc agttgtatca cgatcgcgaa gaagtgacgg tgcacgtccg ccgcatctgg
     7201 gctgcggtgg gtggtcgtcg cgacgagcga taacaggcaa ggccggggcg tcgtcttcgg
     7261 cctgctaccc tttccgtcag gctgttacca aagaagttgc aacctggcgc tcatcagaaa
     7321 taatgtgcgt tacctcatcc agatgcccga tgcgggcttc ccctgaacga ttaaatttac
     7381 ttttatcaat caataacaac gattgcgcgg cacgttttaa tagcatcgat ttgtaatcag
     7441 cgttgatggc attggagtcc cacagtgcgc cgctgctatc gatcccttca caagaaaaaa
     7501 taaacagatc gatttccagc gatttaagtt gggaaatcag cgagggattg acgtaacagc
     7561 catatttgcg ctcaagtgtg ccgccggaac tgatcagttg aatgcgttcg cgtttaccga
     7621 gttcatggca aatcggatgg ctattggtga agacctgaat gttgatgtca ggcaactggc
     7681 gtgccagata ccagcaagtt gaactggcgt ctaaggctat caccatccct tcttcaatcc
     7741 acgcgagcgc ctcgcgcgcg atatctgctt tatgcgcata atggcttttc agccggatgt
     7801 gaaagggatc gccactgtct tgattttgac ggtggatata tttagcgcgt ccatgattgc
     7861 gcagaatttt accctgcgtc tgtaattcat tgagatcgcg acgaatggtt tctttactga
     7921 cctttagctg ttcagagaga gcttccgtgg tcaggctggt atggttcagc agcaggtcga
     7981 ctatcgcttg ctggcgtgcc gctttcatat ttttcacccc atcattcgat aaccgcccgc
     8041 gtcatgcggg cgtaccggta tgagattacg gtgttacccc tttttttaaa agaatattcc
     8101 cgtacttcgc tcgttcgcct gtgataacga tcgcaaaggc tttttgcgcc cgttcataaa
     8161 acgcaaaacg attgatgcgg atgatgtcag gacacggggc ttgtagtgaa agcgcattac
     8221 ggtaacgtcg ttctacttca ggatcgagag tgtcaccttc taccgccgcc atcatcacca
     8281 gcggcggtgc ataactgtcc agttcaaata acgggataat cgcctggagc aagtcgctca
     8341 ccaacaggcc atcagcgcgg atcacctgcg gtcccatcga atgggcggga aagtgagcat
     8401 cggaaaaaat aatttcatct ccatgtccca tctctgccag cactttcaat agttcgggag
     8461 aaattaacgg cgaaattgtt ttcagcattt cacacttcct ctataaattc aggttcagtt
     8521 tgcgggtaga aataacggta ctgataatga atctgtgcgc gggcttcttc cgggctgtta
     8581 aattccccta cgccatacca accgaacagc gcagctcctg cgacggtcgt ttcggcgtcg
     8641 tcgagaactt ttaccggaat atcaagcata ttggctttaa tctgattcca caatgtgttg
     8701 cgacttcctc caccgactaa caataattca gaggccttaa agtgcccgat tttttccagc
     8761 aactgtagat tgcgctgtaa ttgcgcagtt aacccttcca gcgccgcgcg atagaaatgc
     8821 ccccgcgtgg tattaagcgt cactccttgc cagccagcgt tctgacacga caataaatca
     8881 cactgcattt ttacgccatc cgcgccaggc gcgatcagac gagcttcttc aatcaacatt
     8941 tgccagggtg tttcagccgt ccagaacagt tttctcaccc attccagcac gccggatgcc
     9001 agccattgca tacctgggtt atacaaccct gcctggctat ccagttcgca ggtggaaccg
     9061 gcgtactgac ttaacagcga agtatcaacc tgggcgctac ggaccattaa aatttcccat
     9121 gtaccggaag agagcacggg ttcattttgg tcggcaccag cgccaaaaag ggcgaactgg
     9181 gtatcgtgac ctgcggaaat caccggtatg ccaacgggta agccgagcat tgctgcggcg
     9241 ctgttctgta gcgtaccaat ctgttcaccc gcttccacca gacgagggaa gagtcggcgt
     9301 ggaataccgg tggcttgtaa aatttgcgga ctgaaatcgc gttgctggat atccagcatc
     9361 tggctggttc cggccatcgt gatatcagta gtgaattcgc cggttaaacg gtggttaatc
     9421 agcgacgaaa taaagagcca ggcgtgcgcg cgttccagca gttgtggatg attttctttc
     9481 aaccacacca acttatataa cgtattgaaa ctaaaggctc cgacgccaga aatagcctgc
     9541 aaccgctgtg cggagatcaa ctgttcaata ttgtccataa ccgctgctgt tcgcggacat
     9601 ttccagctaa tgatcggata gagcagattg ccttgcttat ctaccagagc gccatccaca
     9661 ccaaaggtgg tgacggcgat accgcggatg tggcattcag tcagttcact attgatttgc
     9721 cgacagcaat cagcaaagcg ttgcaaaatg gcgtctaaag accactggtg ccaggtgttg
     9781 ttttccatcg cgatatcgct ggcattaggc gttgaggcgc gggcaacaat ttttccctgc
     9841 cgattaaccg cgatggccct gacattggtc gcgccacagt cgagtaccag gataacttct
     9901 tgtttcataa tcgctcctgc aatatagccg gataacattg cttatccggc taaccactct
     9961 tgcttgttca ggcccggtag ccggagcgac cgggcatcac atcagggagt aatgtattaa
    10021 cgcttgtaca acgggccgta gttctggcaa gcgcggtaat cctgaccttc aatatccatg
    10081 ccgtgcgcgg cccaggcaga aggacgatat actttggtct cttcaacgtt gtgcatacat
    10141 accgggatac gcagcatgga ggcgagagtg ataaagtctg cgccaacgtg gccgatggtc
    10201 agaaccccgt ggttagcgcc ccagttcgcc attaccgaat acacatccgt aaacggccct
    10261 ttaccggtga ggcgcggtgc aaaccaggtg gttggccagg ttgagttggt gcgtttgttg
    10321 aggatgtcat gcacatcctt cggcaattcc acgctccagc cttccgcgat ttgcagtacc
    10381 ggtcccaggc ctttgatgat gttgacacgg gtcatggtga acgggacgcc gccttcggta
    10441 aggaagcggg aagagtaacc gccgccacgg aagtattcgt ggatcgccgg gcaccattcg
    10501 gtagcggcga ggcaagcgtc agcctctttc tgagagattt cccagtgtgg cttcatcgtc
    10561 gggttacctt cgctgtcgcg ttgtttacag gaaccgtcca gcgcagcaga accggagttg
    10621 atcaaatgga tgatgccgtg ttctgccagt ccatcgagtt tatgccccgt tacacgctca
    10681 attgcttctg gtgaccagta ggtacgcaca tcggcaaata cctgagcggt gccggtgagc
    10741 tggtgaccca ttagcattgc cacgccgtta agactgtcgt tttcggtcgc cacgacaaag
    10801 ggttcgcgca cgccattcca gtcaaatgaa ctgttaagga tcgcttcggc ggtgtcacca
    10861 ttgggatatt gatcggtcca gtgacgttgc ccctggaagc ccgcagcgat ggcgttgtag
    10921 ccaagtgatt cttccacgcg accaatatcg gccagtttgc tgttgccttg catcatgtcg
    10981 cggatacaca tcgccatcag taaactttcg cgcagaactg cgcggctttg ctcggcattg
    11041 cgttgatact gtttgttatt ttcatcttcg ccatagcgga agtttttatc agcccaggcc
    11101 agtgccattt ccagttcggc ttcgtcgtaa atcttctgat cgatacggcg acgcagttcg
    11161 gtcatatcca ccgcctggac tttcattccc agccaggatt caaagaagtt gtgatcaaca
    11221 atggaaccgg cgatacccat cgaaacgccg cccagcgaca gatagctttt acctttcatg
    11281 ctggcgacgg ccaaaccggc gcgggcaaag cgcagcagtt tttcttcaac atcggcagga
    11341 atcgatgtgt catcggcatc ctgaacgtca tgaccgtaaa tggagaatgc cgggatgcct
    11401 ttctggctgt gagctgccag agccgctgcc aggtaaacag cgccgggacg ttcagtgccg
    11461 ttaaagcccc aaatggcttt cgggcgggtt ggatccatgt cgatggtttc actgccatag
    11521 caccagcaag gcgttaccgt aatggtgagg cctacattct gactgctgaa tttttcttcg
    11581 caagcagcgg cttcagccat acccgcgata caggtatcgg aaatgacaca ctcgacggca
    11641 gctccgcagg catggcgcag tttctcggtc agcagtgcgg ccgtagcttt cgccatattc
    11701 attgtttgtt cttcaagcga ctcacgaaca cccatgcgac gaccgtcaat aaccgggcgg
    11761 ataccaattt tcggtaagct gatttttttc attacagatt cctcacttta ttcggaaaat
    11821 atgttcagtt agttgccgtt tgagaacgga aacgggcaaa gataaagatg accgcgaagc
    11881 agagtgcggg gatcagttca gcagtgggga tgttgcccgc cgcgtcactg acaaaaccca
    11941 tgaccggagt gacaataccg ccgccaatga tggtcataac gatgaaggac gaaccgtatt
    12001 tggtgtcctg gccgagattc ttaatgccca gcgagaagat tgttgggtac tgaatcgaca
    12061 taaaggcgct gcataaagtc agggctatta agcccacatg accgccagcg aaggctgaga
    12121 tcaggcacag tgccatagcg attaatgcgt aggcggccag gactttgtgt ggtgcgaagc
    12181 gactgatgag ccaggtaccg gtgaaacgac caataaagaa gcacaccatg gttccggtta
    12241 aatagttagc ggcaaagccg gcagtcatac ctggaatttc ttctacagcg tagcgaatca
    12301 aatagctcca gcaggccgtt tgtgcgccga catagcagaa ttgcgctaat accgcccagc
    12361 gccagtgacg aatacgcgcc agacgagaaa gcgatgcgga gaacgatcct tgtttggcgt
    12421 cactgtgatt atcactctgc aatgccggga atttcgtcag catgatcagc agggcgacca
    12481 gtaacacgat agccacgatg atcatataag gtgtctgtac cgataatacc aggctgtgtt
    12541 tatacgcact caattgctct ggagacattt tatcgagaac gtcttgcgat tgatgtggca
    12601 cgttagacaa aataagactt tgcccaaaga caaccgcgat aattgcgcca aacgagttaa
    12661 atgtttgcgc aagatttaag cggaagtgac cactgctttc tggccctaat accgtaacaa
    12721 aagggtttgc ggcagtttcc agacaaccta atccggctgc aataataaat aggccaacta
    12781 aaaataaggt gtagttcatt atttctgcag cgggccagaa taatgcagcg cccaaggcat
    12841 ataaaaataa tccggtaata atccctgctt tataactgag ttttttcatc aatatcccag
    12901 cagggattgg gataatgaaa taaccaaagt aaaaggccga ttggatcagg ccagcctgga
    12961 aatttgtcag cgtaaaagcc tgctggaatt gaggtaataa aatgtcgtta aggttattgg
    13021 ctaccgccca aagaaaaaac agtgagcaca gcagcgcgaa tggaataatg taacttctgc
    13081 tttgccctgc atctttatct accgcacggt aactctgcgt ttgtattgat gtgtttccca
    13141 tagcatcctc ttaggttcag aagcttaagc acgtaatcgg ttatgttttc aaaagtgcaa
    13201 aaccagcgag ttcgggcttc atatcagtga atatttgcct gtctgaaatg aactcatgga
    13261 ttcattttca aataaaaaac gctctgtaat atgacggcgg tcacacttat tagctggaag
    13321 gtgactttat gtgactacca tcactttaat aatcgaaatt tcatgcccgt ttcagaaaat
    13381 aaatcataaa aaacacaaac cagatgaccg tattaattgt ttatgccgaa aacgggcatt
    13441 cctggcttgg gtgaatagaa aaatgaccgt tttgataaat atttttaaga gcggtcattt
    13501 ttaatggggt tataaaaaga gaacttgcag ttatttactg tgattagttg aaccaggtca
    13561 caaaatataa gcttgtgaat ttctttcttg ttaatcagga agaagatggt gggtaattaa
    13621 acggctaatt caatagtgtg aaaggaacaa cattattgcc ctgttttgaa tcagagagag
    13681 gtagctaatg gaacgaaata aacttgctcg tcagattatt gacacttgcc tggaaatgac
    13741 ccgcctggga ctgaaccagg ggacagcggg gaacgtcagt gtacgttatc aggatgggat
    13801 gctgattacg cctacaggca ttccatatga aaaactgacg gagtcgcata ttgtctttat
    13861 tgatggcaac ggtaaacatg aggaaggaaa gctcccctca agcgaatggc gtttccatat
    13921 ggcggcctat caaagcagac cggatgccaa cgcggttgtt cacaatcatg ccgttcattg
    13981 cacagcagtt tccattctta accgaccgat ccccgctatt cactacatga ttgcggctgc
    14041 aggtggtaat tctattcctt gcgcgcctta tgcgaccttt ggaacacgcg aactttctga
    14101 acatgttgcg ctggctctca aaaatcgtaa ggcaactttg ttacaacatc atgggcttat
    14161 cgcttgtgag gtgaatctgg aaaaagcgtt atggctggcg catgaagttg aagtgctggc
    14221 gcaactttac ctgacgaccc tggcgattac ggacccggtg ccagtgctga gcgatgaaga
    14281 gattgccgta gtgctggaga aattcaaaac ctatgggtta cgaattgaag agtaatttcg
    14341 taaagcaaca aggagaagga tgatggctaa cagaatgatt ctgaacgaaa cggcatggtt
    14401 tggtcggggt gctgttgggg ctttaaccga tgaggtgaaa cgccgtggtt atcagaaggc
    14461 gctgatcgtc accgataaaa cgctggtgca atgcggcgtg gtggcgaaag tgaccgataa
    14521 gatggatgct gcagggctgg catgggcgat ttacgacggc gtagtgccca acccaacaat
    14581 tactgtcgtc aaagaagggc tcggtgtatt ccagaatagc ggcgcggatt acctgatcgc
    14641 tattggtggt ggttctccac aggatacttg taaagcgatt ggcattatca gcaacaaccc
    14701 ggagtttgcc gatgtgcgta gcctggaagg actttccccg accaataaac ctagtgtacc
    14761 gattctggca atccccacca cagcaggcac tgcggcagaa gtgaccatta actacgtgat
    14821 cactgacgaa gaaaaacggc gcaagtttgt ttgcgttgat ccgcatgata tcccgcaggt
    14881 ggcgtttatt gacgctgaca tgatggatgg tatgcctcca gcgctgaaag ctgcgacggg
    14941 tgtcgatgcg ctcactcatg ctattgaggg gtatattacc cgtggcgcgt gggcgctaac
    15001 cgatgcactg cacattaaag cgattgaaat cattgctggg gcgctgcgag gatcggttgc
    15061 tggtgataag gatgccggag aagaaatggc gctcgggcag tatgttgcgg gtatgggctt
    15121 ctcgaatgtt gggttagggt tggtgcatgg tatggcgcat ccactgggcg cgttttacaa
    15181 cactccacac ggtgttgcga acgccatcct gttaccgcat gtcatgcgct ataacgctga
    15241 ctttaccggt gagaagtacc gcgatatcgc gcgcgttatg ggcgtgaaag tggaaggtat
    15301 gagcctggaa gaggcgcgta atgccgctgt tgaagcggtg tttgctctca accgtgatgt
    15361 cggtattccg ccacatttgc gtgatgttgg tgtacgcaag gaagacattc cggcactggc
    15421 gcaggcggca ctggatgatg tttgtaccgg tggcaacccg cgtgaagcaa cgcttgagga
    15481 tattgtagag ctttaccata ccgcctggta aatgcgctga tgtgataatg ccggatacga
    15541 cgtttgcggc gtatccggct cgccgttacc gtaccaaccg caattgctgt aaattgccgt
    15601 cgatatgcaa atcggtttgt aagcgggcaa tatcgcggca cagaaacgcc atctctttat
    15661 gggtttctaa ttttttgcgc cacttttcgg caaccgcatc cagattctca tatattcctt
    15721 ccagactctg aaactcgacc agcagctgcg tggcgctttt tggtccgatt cccacaacac
    15781 ccggtacctt tgaactgctg atccccgcaa gtccccagta atcgggcaac tgctgcggtt
    15841 gaacgccaaa ttctttatcg ataaatggcg catccagcca acgtttctgg aagtaatcac
    15901 gaatacgtaa tgtcggtgaa agtaactgac agtagccttt atctgtcgaa acaatcgttg
    15961 cctgatgccc ggcctgtgtc actttgaccg ccagcgtggc ggctaagtca tcggcttcgt
    16021 tgccgctggt tgaccagcac gggacgccgc gttgctcaaa ggcggcgcgt aatgcaggca
    16081 tctcgtcgtg caactcttcc ggcattggcg gtcgacccgc tttgtaatct ggtaaacgct
    16141 gatgacgcca gccgctactg cggttttcat catcaaaaac ggcgaccgcg tgggttggct
    16201 ggctgtgcat aatgagctga tcgagcgcat gctggcaggt ttcgacacag ggcgatccct
    16261 gaacggcatg aatgcgacga ataagattca gtgcatcgac aataagcaaa tgaacagcca
    16321 cgataacccc cttaaagatc aggcgataag ggtaacattc gatgccggaa caggctacgg
    16381 ctggatgaga aatcgggaag aggcctcgca aaacgaggcc tctggagagc gattaatcgc
    16441 aggcaacgat cttcattgcc aggccgccgc gagaggtttc gcggtacttg gcgttcatat
    16501 ctttacctgt ttcgtacatg gtttcgataa ctttatcgag gcagacgcgc ggctcgctgg
    16561 tacggcgtag cgccatacgt gcggcgttca ccgcttttac tgccgcaatg gcgttacgct
    16621 cgatgcatgg cacctggacc tgtccggcga ccgggtcaca cgtcagaccg aggttgtgct
    16681 ccatggcgat ttccgccgcg atgcacacct gcgccgggct tgcacctaac agttctgcca
    16741 gaccagccgc cgccattgag cacgccacac caacttcacc ctggcagccg acttcagcac
    16801 cagaaattga cgcgttcatc ttataaagag aaccaatggc actggctacc agcaggtaac
    16861 gagccagtga gttagcgttc acttcgcgga taaacttatc gtagtacgcc agcacagccg
    16921 ggataatccc gcacgcaccg ttagtcggcg cagtcaccac gcgaccgcca gcggcgttct
    16981 cttcgttcac tgccagtgca aacatgttga tccagtcaac aaccgccatc gggtcagtgg
    17041 tggttttatc ctggctgacc agcatccggc gtagtgccgc agcacggcgt ggtacgcgca
    17101 gtttgccagg caacacgcct tcggtagaaa taccgcgctc aataccgccg cgcatgactt
    17161 cccagacgtt cgccaggtgc tgttccagct cttctttgct gtgcagcgcc agctcgtttt
    17221 tcatcatcag gccagagagt gacagcccgg tttcctgaca atgtttttgc agatcggctg
    17281 ctgaactgta cggataagga acttcaaccg gtgcgctatc ctgctggcca aaatgttctt
    17341 catcaacgat aaagccaccg ccgatagagt agtaagtctg gctgtaaatg actttatcgc
    17401 ccgccagtgc ggtaatgcgc ataccgtttt catgcagaga aaggttgtcg gcatggaagt
    17461 tcatgcactg atcaaccggg aactccactt catgctgacc gtttgccagc atcaggcgac
    17521 catgagtatt cacatcctga ataaaaccgg ggatggaatc gatatccacg gtatccggca
    17581 ggttacccgc caggcccata ataatggcga tatcagtgtg gtggccttta ccggtcagag
    17641 agagcgagcc gtacacgtca accaccacgc gggtcacgtc tttaagcagg ttacgggcaa
    17701 tcagatcgtc ggtaaattgt ttacccgctt tcattggtcc aacggtatga gaactggaag
    17761 ggccaatgcc gattttgaaa atatcgaata cgctaatcat aggaaataca tcgcgttaaa
    17821 acggaggaag cgccgcccga aagcggcgcg aaaggactta gctgaacaga gagtagaaga
    17881 ttgcggagat tgcaatcaga cccatcacga caacgaatac gttgctgatg tgaccgctgt
    17941 acttacgcat tgccggtact ttctgaattg cgtacatcgg catcaggaac aggatcatcg
    18001 cgatgattgg gccgcccagg gtttcaatca tacccaggat gctcgggttc agggtggcaa
    18061 caatccaggt cgttaccagc atgaacagcg cagtgatacg gttcagcttg ttgatttcga
    18121 tagacttacc tttaccacgc agagatttaa tcaccatacc gttgaagcct tcacgtgcgc
    18181 ccaggtagtg gccgaggaag gatttggtga tagcgataat cgcgataatc ggagccatcc
    18241 acgcgataac cggtgcgtta aagtggttag ccaggtaaga cagaatcgag atgttctgct
    18301 ctttagccgc agccaggtct gccggagtca ggctcagtac gcagctgaag acgaagaaca
    18361 ttacggtcag caccatcatg atgtgtgcga atgccaggat cttcgagcat ttctgttctg
    18421 ccatatcgcc gtactcttca cgcttcgcaa cggcgaaaga agagatgatc ggagagtggt
    18481 tgaacgagaa caccattacc ggaattgcca gccacagggt catccacaga ccgtttccgg
    18541 ttgcagatgc agtgtccaga gacagcgttt ccagtgctgc gccgttccac tgcgggatga
    18601 ggtacagagc cagcagcatc agtacgccaa caaacgggaa taccagaata ctcatcgctt
    18661 taacgatcat ctgctcaccg aagcgaacga tggtcatcat acccacgatc aggatcagcg
    18721 acagaatcgc acgcggcggt ggcgtcatac ccagctggtg agacatgaag ctttcaacgg
    18781 tattggtgat tgccacgcta taaaccagca ggatcgggta gatagcgaag aagtagagca
    18841 gggtaatcag tttacctgcg ccaataccaa agtgttcttc tacaacctcg gtgatgtctt
    18901 cgcccgggtt tttaccagac agtacgaagc gagtcaggcc gcggtgagca aaaaacgtca
    18961 tcgggaacgc aaggatagcc atgatgatca gcgggatcat accgccaaca ccggcgttga
    19021 ttggcaggaa cagcacgccc gcgccgattg ccgtgccgta aaggcccagc atccacatgg
    19081 tgtctgtctt gcgccaggca ctacgagagt ctttcgacgc aatcgtgctg gtttgagtcg
    19141 tttccatcta tttctcctgg aggaatgcaa ataattcagc ttttagccag ttcagcccgg
    19201 gaaggtgcct gaccgtaaaa atgaaatctg tctatcggcg atggcttaat catttaccgc
    19261 cgtaataatt tgagggcgga aagatacaca caagttatgt atctatcagt gatcttgatc
    19321 ccaaatacaa taatctactt ggaagataga ttatcccaac gctttactct gtaaatcaaa
    19381 agtaaaagag aatttaaggg caatatttgc ccattaaacg acatctgact tcttccgggg
    19441 cgcgcaatat atgtaggttg aactgaaaaa atcaatactt ttagttaatc atggcgataa
    19501 tttcttt
//
LOCUS       DAMZET010000078        19125 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.078, whole genome shotgun sequence.
ACCESSION   DAMZET010000078 DAMZET010000000
VERSION     DAMZET010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.078"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            206..1345
                     /locus_tag="POB34_002761"
     CDS             206..1345
                     /locus_tag="POB34_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HDI5919671.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(1337..2617)
                     /gene="csiE"
                     /locus_tag="POB34_002762"
     CDS             complement(1337..2617)
                     /gene="csiE"
                     /locus_tag="POB34_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HDI5919672.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(2808..3662)
                     /locus_tag="POB34_002763"
     CDS             complement(2808..3662)
                     /locus_tag="POB34_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI5919673.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            complement(3807..4610)
                     /gene="suhB"
                     /locus_tag="POB34_002764"
     CDS             complement(3807..4610)
                     /gene="suhB"
                     /locus_tag="POB34_002764"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HDI5919674.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            4729..5466
                     /gene="trmJ"
                     /locus_tag="POB34_002765"
     CDS             4729..5466
                     /gene="trmJ"
                     /locus_tag="POB34_002765"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HDI5919675.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            5767..6255
                     /gene="iscR"
                     /locus_tag="POB34_002766"
     CDS             5767..6255
                     /gene="iscR"
                     /locus_tag="POB34_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HDI5919676.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            6367..7581
                     /gene="iscS"
                     /locus_tag="POB34_002767"
     CDS             6367..7581
                     /gene="iscS"
                     /locus_tag="POB34_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDI5919677.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            7609..7995
                     /gene="iscU"
                     /locus_tag="POB34_002768"
     CDS             7609..7995
                     /gene="iscU"
                     /locus_tag="POB34_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HDI5919678.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            8012..8335
                     /gene="iscA"
                     /locus_tag="POB34_002769"
     CDS             8012..8335
                     /gene="iscA"
                     /locus_tag="POB34_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HDI5919679.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            8431..8946
                     /gene="hscB"
                     /locus_tag="POB34_002770"
     CDS             8431..8946
                     /gene="hscB"
                     /locus_tag="POB34_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HDI5919680.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            8963..10813
                     /gene="hscA"
                     /locus_tag="POB34_002771"
     CDS             8963..10813
                     /gene="hscA"
                     /locus_tag="POB34_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HDI5919681.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            10815..11150
                     /gene="fdx"
                     /locus_tag="POB34_002772"
     CDS             10815..11150
                     /gene="fdx"
                     /locus_tag="POB34_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HDI5919682.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            11162..11362
                     /gene="iscX"
                     /locus_tag="POB34_002773"
     CDS             11162..11362
                     /gene="iscX"
                     /locus_tag="POB34_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HDI5919683.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            11540..12823
                     /gene="pepB"
                     /locus_tag="POB34_002774"
     CDS             11540..12823
                     /gene="pepB"
                     /locus_tag="POB34_002774"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HDI5919684.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            12965..13741
                     /gene="sseB"
                     /locus_tag="POB34_002775"
     CDS             12965..13741
                     /gene="sseB"
                     /locus_tag="POB34_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HDI5919685.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGGEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPSDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(14234..15079)
                     /gene="sseA"
                     /locus_tag="POB34_002776"
     CDS             complement(14234..15079)
                     /gene="sseA"
                     /locus_tag="POB34_002776"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HDI5919686.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            15286..>19125
                     /gene="yfhM"
                     /locus_tag="POB34_002777"
     CDS             15286..>19125
                     /gene="yfhM"
                     /locus_tag="POB34_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HDI5919687.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGLTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAGFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPDETVADQQKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGN"
BASE COUNT         4619 a         4778 c         5312 g         4416 t
ORIGIN      
        1 tttcatcgcc attgtgcagg aacgtttggc aagcgtaacg gcataattac gcattatttt
       61 tgcaacatgc gtcgcggatc tcagtttttt agtgcttgtg aactggcgtt acagacatcg
      121 ggaagcaggc tcgaatattc ttatttttca acttgcacca acattcgatt aaccccagac
      181 taacgcctcc tgacgggagg gactcatggt tttgcaatcc acgcgctggt tggcgctcgg
      241 ctatttcaca tactttttta gttacggcat ttttctacct ttctggagcg tctggcttaa
      301 agggattggt ttaacgccag aaaccatcgg cctgttattg ggggcaggtc tggttgcccg
      361 tttcctcggg agtttgctca tcgcgccccg cgtcagcgat ccttcccgcc tgatttccgc
      421 cttgcgcgtg ctggcactgc tgacacttct ctttgctgtc gccttctggg cgggggcgca
      481 cgtagcgtgg ctgatgctgg tgatgattgg ctttaacctc tttttctcac cactggtacc
      541 gttgaccgat gcactggcga atacgtggca aaagcagttc ccgcttgatt acggcaaagt
      601 gcgactgtgg ggctcggtgg cgtttgtcat tggctcggcg ctgacgggca aactggtcac
      661 tatgtttgat tatcgggtga tcctcgcgct gttgacgttg ggcgtggcat ccatgctgct
      721 cggctttctc atccgtccga cgattcagcc acaaggggca agtcgccagc aggagagcac
      781 cggttggtct gcgtggttgg cgctggttcg ccagaactgg cgctttctgg cctgcgtttg
      841 tttattgcag ggggcacatg cggcctatta cggttttagc gccatttact ggcaggcagc
      901 tggctactcg gcctcggcgg tggggtattt gtggtcgctg ggcgtggtgg cggaagtcat
      961 tatctttgcg ctgagtaata aacttttccg ccgttgtagt gcacgcgata tgctgttgat
     1021 ctcggcgatt tgcggcgtag tgcgctgggg cattatggga gcaactacgg cgttgccgtg
     1081 gttgatagtg gtgcaaattc tgcattgcgg caccttcacg gtctgccacc tggccgccat
     1141 gcgttatatt gctgctcgcc agggtagcga agtcatccgt ttacaggcgg tttactctgc
     1201 cgtcgcgatg ggcggcagta tcgctatcat gaccgttttc gccggtttcc tgtatcaata
     1261 tctgggccac ggcgtgttct gggtaatggc gctggtggcg ctgccggcaa tgtttttgcg
     1321 cccgaaagtt gttccctcat gctgattcga gcattttgcg gatctgctcc tgttgatgtg
     1381 ttgtcagcgt caggtccgta tagatcagcg gtggtgaaaa gagcggtaat ggcatggtat
     1441 aaggcgcaat aatcagtgcc gcgccacgcg gagcgcctgt ctgcaaaaat gcctttaccg
     1501 acatatgctt aatgttgagc ggcagtaacg ttagttcgcg taactgctgc tcaatctgcg
     1561 cttctcgctc gctatcattc ccggtcagta gaataatctg tttttcatgc agatcgtttt
     1621 cctgcattag ccaggcaccg aaaatcaccg cgaccagacc actttcctca tcagaaagat
     1681 ggacgccgta ttcactttca aatccggcca gcgccgcgcg ggtggtgcgc accaggcgtg
     1741 ggtacagtct ggcgaactct tccggcaggg tattatcaat accgatggca aacaaactgc
     1801 gatttaacgc ctgggcgagg tgggtataaa gctgatcaca taacccctgt tcatcataaa
     1861 aacgaacatt tcccagctca cgaaaatggt ttaccagacg tttgatagac tgacgcaatt
     1921 gtctgtctcg ctgatgcgca tcccgcagtg gatcgggaac ccgcaacatg gaaaaaagta
     1981 gtgccataaa cagtggctca tcaggcggta caggctggag cgcccgacgc tgccagtggc
     2041 gtccaatttc ctgcgctacc tgaaattcaa ggcaggattc cgcccagcga cgttggagcg
     2101 gattaaactg aggcgtaatt ccagcgtggt gctgcagcaa acaatattgc agatacagac
     2161 acaggaaatg aatatcgcgc gattcaaagc gtttttgcag ccgtcgggag cagagattca
     2221 ctaacgcttg tagattggta tcgtcgtaaa agttacgcgc aattccgcgc cgcttcagtt
     2281 cactcttcag ggcgggggtg aattggctgg taataaatga cggacacaga cggaaaccac
     2341 gtcgtagcca gtgaaataaa cacaaacgtt ggttaagcac tgtaccttca acccggtagc
     2401 taccgtcata accagtcgtc agtgtgagtt gatgatagcg caggatctcc cgtcctgttt
     2461 cgctgatatc aagactggca atatcatcat ccacaccatt aagctcgctg aaggttgcca
     2521 tggtggcggt caaccccggc tgaaagagcg tcagcaagat ctggcagcgg cgctggggag
     2581 ccgaaaggac agatggtgga gcaagcgtag gcatcatctg tagatctccc ataagcataa
     2641 ttgataagaa tagctaaagt ttgctcaggg aaatggcgaa gggaatgcta atcatcagaa
     2701 atgttggcaa gcgtcacaga atttttctca tcttaaactg ttctgaccag tggattagca
     2761 tgtcttaagg tggttttgtg aatgattatc gtgctagcga tcatgggtta gttctgcgga
     2821 ttcaacgcgc taaggataaa gtccaccatc tgttcgcgat aaacatcgcc gtggcgatcg
     2881 gaaaaagcat caatgtgttc gccatccggg attaggatca accgttttgg ctcttttgcc
     2941 aggctataca acttttcgct gtgctgccat gggataacgt gatcagcttt accgtgaatg
     3001 agtaaaagcg ggatcgggct gacgctggcg atataatttt cgccgctgta actctcatca
     3061 agtaagtagc cactgccggg gatcatctgg ttggcgatgg ttgcataaga ggcaaatgtg
     3121 gagtcgagga tcaccgcacg tatgccttca cgatcaccct gaccaataac agcaagaata
     3181 ttcgccccgc caatactctg cccgaacagc accagccgtt gtgggtttac atcactgcga
     3241 tggcgcacca cattgatggc gctttgcgta tcgtccagca atccggcctg ggacggcgtg
     3301 ccttttgatt taccaaaccc gcgataatca aacataaaaa cgttgaaatt acgctcgggt
     3361 aaccaactga ccagcggcca gtgggcggac atatttccgg cattgccgtg agcatgaatg
     3421 atggttgcga tggcgttgtc agcagggccc gtcgaagaag gaataaacca gccttgcaga
     3481 cgagtaccgt ccttagccgt aaattcgacg gattccgccg accaggggtc gggaccgtaa
     3541 attttatcgt caggataata gaagaagttg atggcaacgc gcggcactaa ataaacgcag
     3601 aaggcaacaa caaagagaat aaacagaatt ttgggaacgc gtttgttcac tggcagtgcc
     3661 ataacctttc cttgcgactt ctttatcatc cattaatcgg ggaaagatgg tgggagagtg
     3721 acgaaagaaa gtaaagcggt gaggaagtat ttcagaatag cgcctgaaag gcgagggcgg
     3781 gtgagtgaca tcacccgcct gagtcattaa cgcttcagag cgtcgcttaa ctcgtcacgc
     3841 atgttcgcca gcatggcttt cacaacacgt gggttaccag caacgatgtt accggtcagc
     3901 atatagttat gaccaccggt gaagtcgctg acgatgccgc ccgcttcacg aaccagcagc
     3961 tcgcctgccg cgaaatccca cggacgcaga ccgatttcaa agaaaccgtc aacacgaccc
     4021 gcagcgacgt aagccagatc cagcgccgca gaaccggtac gacggaagtc tgcacattcg
     4081 ttgaacagtt tgccgacgat gttgatgtag gtagtggcgt actgttttgc tttgaacggg
     4141 aagccggtcg ccagaatggt accgtcgaga tcgcgagcgg tgctaccgcg cagacggtag
     4201 ccgttcagct gtgcgccctg accgcgagtg gcggtgaaca gttcgttacg cataggatcg
     4261 tataccacag caacttcggt gcggcctttg atacgaacgg cgatagatac cgcgaagtgc
     4321 ggcagacgtt tgataaagtt ggtagtacca tccagtggat cgataaccca ttgaacatcc
     4381 tgatcagtac cttcaagttc accgctttct tcggtgatga tggtgtgctg tgggtaagat
     4441 ttacgaatcg tgtcgataat caccgcttcg gcagctttat ctacgttggt cacgaaatcg
     4501 ttactgcctt tctggctcgc ttctacagcg tccggggttt catagttttt ggcaattaaa
     4561 ttacccgcct tgcgcgctgc gcgcacggcg atgttcagca tcggatgcat cggtctctct
     4621 cactggatgt taaagaacgg gaaaacggcg cagagtatag cagcgagatc ggcatctgtc
     4681 ttcgttttat gataagatgc gtgaataatc tttagatgaa gaaagacaat gctgcaaaat
     4741 attcgaattg tgctggtgga gacgtcacac accggcaata tgggttctgt tgcccgtgcc
     4801 atgaaaacaa tgggattaac caatctgtgg ctggttaatc cactggtgaa acccgactcc
     4861 caggcgattg ccctggcagc aggggccagc gatgtgattg gtaatgctca catcgtcgat
     4921 acgctcgacg aagcgttagc cggttgtagc ctggtggtgg gcaccagcgc acgctcccgc
     4981 acgctgccat ggccgatgct cgacccgcgc gaatgcggcc tgaaaagcgt cgctgaagcg
     5041 gcaaataccc cggtggcgct ggtgtttggt cgcgagcgcg tcggcttgac caatgaagag
     5101 ttgcagaaat gccattatca tgtcgcgatt gcggctaacc cggaatacag ctcgctgaac
     5161 ctggcgatgg cggttcaggt tattgcctat gaagtgcgca tggcctggct ggcaactcag
     5221 gaaaacggcg agcaggtcga gcatgaagag acgccgtatc cgctggtcga tgatctggag
     5281 cgtttttatg gtcatctgga acaaacgctg ctggcaaccg gttttatccg tgaaaaccat
     5341 ccggggcagg tgatgaataa attgcgccgt ctgtttaccc gtgcgcgccc ggaaagccag
     5401 gaattgaata tcctgcgcgg gattctggct tctattgagc agcagaataa aggtaataag
     5461 gcctgagggc taagccagat aaatgcacgg acggtcccct cgccccttcg gggagagggt
     5521 tagggtgagg ggaagaggcc agcgctaatg cgaataattg ccctcaaccc gcccatggtc
     5581 tggaaggaca acaggtacaa acgccacgat aaaaaaatgg cactgaaggt taaatacccg
     5641 actaaatcag tcaagtaaat agttgaccaa tttactcggg aatgtcagac ttgaccctgc
     5701 tatgcaatac ccccactttt acaataaaaa accccgggca ggggcgagtt tgaggtgaag
     5761 taagacatga gactgacatc taaagggcgc tatgccgtga ccgcaatgct tgacgttgcg
     5821 ctcaactctg aagcgggccc ggtaccgttg gctgatattt ccgaacgtca gggaatttcc
     5881 ctttcttatc tggaacaact gttttcccgt ctgcgtaaaa atggtctggt ttccagcgta
     5941 cgtggaccag gcggtggtta tctgttaggc aaagatgcca gcagcatcgc cgttggcgaa
     6001 gtgattagcg ccgttgacga atctgtagat gccacccgtt gtcagggtaa aggcggctgc
     6061 cagggcggcg ataaatgcct gacccacgcg ctgtggcgtg atttgagcga ccgtctcacc
     6121 ggttttctca acaacattac tttaggcgaa ctggttaata accaggaagt gctggatgtg
     6181 tctggtcgtc agcatactca cgacgcgcca cgcacccgca cacaagacgc gatcgacgtt
     6241 aagttacgcg cttaataaaa agaattcaga atcaggccgg agtgctaaat actccgtaaa
     6301 cacggtcgta catccagccg gtagcctggt tccttgcatt gagtgatgta cggagtttat
     6361 agagcaatga aattaccgat ttatctcgac tactccgcaa ccacgccggt ggacccgcgt
     6421 gttgccgaga aaatgatgca gttcatgacg atggacggaa cctttggtaa cccggcctct
     6481 cgttctcacc gtttcggctg gcaggctgaa gaagcggtag atatcgcccg taatcagatt
     6541 gccgatctgg tcggcgctga tccgcgtgaa atcgtcttta cctctggtgc aaccgaatct
     6601 gacaacctgg cgatcaaagg tgcagccaac ttttatcaga aaaaaggcaa gcacatcatc
     6661 accagcaaaa ccgaacacaa agcggtactg gatacctgcc gtcagctgga gcgcgaaggt
     6721 tttgaagtca cctacctggc accgcagcgt aacggcatta tcgacctgaa agaacttgaa
     6781 gcagcgatgc gtgacgacac catccttgtt tccatcatgc acgtaaataa cgaaatcggc
     6841 gtggtgcagg atatcgcggc tatcggcgaa atgtgccgtg ctcgtggcat tatctatcac
     6901 gttgatgcaa cccagagcgt gggcaaactg ccaatcgacc tgagccagtt gaaagttgac
     6961 ctgatgtctt tctccggtca caaaatctat ggcccgaaag gtatcggtgc gctgtatgtg
     7021 cgtcgtaaac cgcgtgtacg catcgaagcg caaatgcacg gcggcggtca cgagcgcggt
     7081 atgcgttccg gcactctgcc tgttcaccag atcgtcggaa tgggcgaagc ctatcgcatc
     7141 gcaaaagaag agatggcgac cgagatggaa cgtctgcgcg gcctgcgtaa ccgtctgtgg
     7201 aacggcatca aagatatcga agaagtttac ctgaacggtg acctggaaca cggtgcgccg
     7261 aacattctca acgtcagctt caactacgtt gaaggtgagt cgctgattat ggcgctgaaa
     7321 gacctcgcgg tttcttccgg ctccgcctgt acgtcagcaa gcctcgaacc gtcctacgtg
     7381 ctgcgcgcgc tggggctgaa cgacgagctg gcacatagct ctatccgttt ctctttaggt
     7441 cgttttacta ctgaagaaga gatcgactac accatcgagt tagttcgtaa atccatcggt
     7501 cgtctgcgtg acctttctcc gctgtgggaa atgtacaagc agggcgtgga tctgaatagc
     7561 atcgaatggg ctcatcatta atcggtatcg gaatcaggag aatttataat ggcttacagc
     7621 gaaaaagtta tcgaccatta cgagaatccg cgtaacgtgg gttcctttga caacaacgac
     7681 gagaacgtcg gcagcggcat ggtgggtgca ccggcctgtg gcgacgtgat gaagttgcag
     7741 attaaagtca acgatgaagg tatcattgaa gacgcgcgtt tcaaaactta cggctgcggt
     7801 tctgctatcg cttccagctc cctggtcacc gaatgggtga aagggaagtc tctcgacgaa
     7861 gcgcaggcga tcaaaaacac cgatattgct gaagaacttg aactgccgcc ggtgaaaatt
     7921 cactgttcta ttctggcaga agacgcgatc aaagccgcca ttgcggacta taaaagcaaa
     7981 cgtgaagcaa aataagagtt gaggtttggt tatgtcgatt acactgagcg acagtgcagc
     8041 agcgcgagta aatacctttc tggctaaccg cggcaaaggg tttggcctgc gtctgggcgt
     8101 gagaacctcc ggatgttcag gtatggctta tgtactggaa tttgttgacg aaccgacgcc
     8161 ggaagacatc gtgtttgaag acaaaggcgt gaaagtcgtg gtcgatggca aaagcctgca
     8221 atttctggac ggtacgcaac tggacttcgt aaaagaaggc ctgaacgaag ggtttaaatt
     8281 caccaacccg aacgtcaaag atgagtgtgg ttgcggcgaa agcttccacg tttgatgcgc
     8341 ataccgataa ccccaccgtg gtcgcctgcg cgtggggttt gttttacctg attcgccgtt
     8401 atcgcggcgg atcgcagccc tgagaatgtt atggattact tcaccctctt tggcttgcct
     8461 gcccgttatc aactcgatac ccaggcactg agcctgcgtt ttcaggatct acaacgtcag
     8521 tatcatcctg ataaattcgc cagcggaagc caggcggaac aactcgccgc cgtacagcaa
     8581 tctgcaacca ttaaccaggc ctggcaaacg ctgcgtcatc cgttaatgcg cgcggaatat
     8641 ttgctttctt tgcacggttt tgatctcgcc agcgagcagc atactgtgcg cgacaccgcg
     8701 ttcctgatgg aacagctgga gctgcgcgaa gaactggacg agatcgaaca ggcgaaagat
     8761 gaagcgcggc tggaaagctt tatcaaacgt gtgaaaaaga tgtttgatac ccgccatcag
     8821 ttgatggttg aacagttaga caacgagacg tgggacgcgg cggcggatac cgtgcgtaag
     8881 ctgcgttttc tcgataaact gcgaagcagt gccgaacaac tcgaagaaaa actgctcgat
     8941 ttttaatttc tggaagctaa acatggcctt attacaaatt agtgaacctg gtttgagtgc
     9001 tgcgccgcat cagcgtcgtc tggcggctgg tattgacctg ggcacaacca actcgctggt
     9061 ggcgacagtg cgcagcggtc aggccgaaac gttagccgac catgaaggcc gtcacctgct
     9121 gccatctgtt gttcactatc aacagcaagg gcattcggtg ggttatgacg cgcgtactaa
     9181 cgcagcgctt gacaccgcca acaccattag ttctgttaaa cgcctgatgg ggcgctcgct
     9241 ggctgatatc cagcaacgct atccgcatct gccttatcaa ttccaggcca gcgaaaacgg
     9301 cctgccgatg attgaaacgg cggcggggct gctgaacccg gtgcgcgttt ctgcggacat
     9361 cctcaaagca ctggcggcgc gggcaactga agccctggca ggcgagctgg atggtgtagt
     9421 tatcaccgtt ccggcgtact ttgacgatgc ccagcgtcag ggcaccaaag acgcggcacg
     9481 tctggcgggg ttgcatgttc tgcgcttact taacgaaccg accgctgcgg ctatcgccta
     9541 cgggttggat tccggtcagg aaggcgtgat cgctgtttat gacctcggtg gcgggacgtt
     9601 tgatatttcc attctgcgct taagtcgcgg cgtgtttgaa gtgctggcaa ccggcggtga
     9661 ttccgcgctc ggcggcgatg atttcgacca tctgctggcg gattacattc gcgagcaggc
     9721 gggcattcct gatcgtagcg ataaccgcgt tcagcgtgaa ctgctggatg ccgccattgc
     9781 agccaaaatc gcgctgagcg atgcggactc cgtgaccgtt aacgttgcgg gctggcaggg
     9841 cgaaatcagc cgtgaacaat tcaatgaact gatcgcgcca ctggtaaaac gaaccttact
     9901 ggcttgtcgt cgcgcgctga aagacgcggg tgtagaagct gatgaagtgc tggaagtggt
     9961 gatggtgggc ggttctactc gcgtgccgct ggtgcgtgaa cgggtaggcg aatttttcgg
    10021 tcgtccaccg ctgacttcca tcgacccgga taaagtcgtc gctattggcg cggcgattca
    10081 ggcggatatt ctggtgggta acaagccaga cagcgaaatg ctgctgcttg atgtgatccc
    10141 actgtcgctg ggcctcgaaa cgatgggcgg cctggtggag aaagtgattc cgcgtaatac
    10201 cactattccg gtggcccgcg ctcaggattt caccaccttt aaagatggtc agacggcgat
    10261 gtctatccat gtaatgcagg gtgagcgcga actggtgcag gactgccgct cactggcgcg
    10321 ttttgcgctg cgtggtattc cggcgctacc ggctggtggt gcgcatattc gcgtgacgtt
    10381 ccaggtcgat gccgacggtc ttttgagcgt gacggcgatg gagaaatcca ccggcgttga
    10441 ggcgtctatt caggtcaaac cgtcttacgg tctgaccgat agcgaaatcg cttcgatgat
    10501 caaagactca atgagctatg ccgagcagga cgtaaaagcc cgaatgctgg cagaacaaaa
    10561 agtagaagcg gcgcgtgtgc tggaaagtct gcacggcgcg ctggctgctg atgccgcgct
    10621 gttaagcgcc gcagaacgtc aggtcattga cgatgctgcc gctcacctga gtgaagtggc
    10681 gcagggcgat gacgttgacg ccatcgaaca agcgattaaa aacgtagaca aacaaaccca
    10741 ggatttcgcc gctcgccgca tggaccagtc ggttcgtcgt gcgctgaaag gccattccgt
    10801 ggacgaggtt taatatgcca aagattgtta ttttgcctca tcaggatctc tgccctgatg
    10861 gcgctgttct ggaagctaat agcggtgaaa ccattctcga cgcagctctg cgtaacggta
    10921 tcgagattga acacgcctgt gaaaaatcct gtgcttgcac cacctgccac tgcatcgttc
    10981 gtgaaggttt tgactcgctg ccggaaagct cagagcagga agacgacatg ctggacaaag
    11041 cctggggact ggagccggaa agccgtttaa gctgccaggc gcgcgtcacc gacgaagatt
    11101 tagtggtcga aatcccgcgt tacactatca accatgcgcg tgagcattaa cagaggttag
    11161 tatgggactt aaatggaccg atagccgcga aattggcgaa gcactgtacg atgcgtatcc
    11221 cgatcttgat ccgaaaacgg ttcgattcac cgatatgcat cagtggattt gcgatctgga
    11281 agatttcgac gacgacccgc aggcatccaa cgagaaaatc ctcgaagcga ttttgttagt
    11341 ctggctggac gaggccgaat aatttccgcg tcatgcttca cgccgcagat gcgttggctg
    11401 cgctcgttca ccccagtcac atagttatct atgctcctgg ggcttcactc acttgccgcc
    11461 ttcctgcaac gcgaatcatt tagcggaaaa atcctggggc tgccaactgg cggccctttt
    11521 acaaagaagg ataactaaaa tgacagaagc gatgaagatt accctctcta cccaacctgc
    11581 cgacgcgcgc tggggagaaa aagcaactta cagcattaat aatgacggca ttaccctgca
    11641 tttgaacggg gcagacgatc tggggctgat ccagcgtgcg gcgcgcaaga ttgacggtct
    11701 gggcatcaag catgttcagt taagcggtga aggctgggat gcggatcgct gctgggcatt
    11761 ctggcaaggt tacaaagccc cgaaaggcac gcgtaaagtg gagtggccgg atctggacga
    11821 tgcccagcgc caggaactgg ataaccgcct gatgatcatc gactgggtgc gtgacaccat
    11881 caacgcaccg gcagaagaat tgggaccatc gcaactggca cagcgtgctg ttgatctgat
    11941 cagcaacgtc gcgggcgatc gtgtgactta tcggatcacc aaaggcgaag atctgcgtga
    12001 gcaaggttat atggggctgc acaccgtcgg gcgcggttca gaacgttctc cggtattgct
    12061 ggcgctggat tacaacccaa ctggcgataa agaagcaccg gtgtacgcgt gcctggtagg
    12121 taaaggtatc acttttgact ccggcggcta cagcatcaaa cagactgcgt ttatggactc
    12181 gatgaagtcg gacatgggcg gcgcggcaac ggttaccggg gcgctggcat ttgccattac
    12241 gcgcggactg aacaagcgcg tgaagctgtt cctctgctgt gcggataacc tgattagcgg
    12301 caatgcgttc aagctgggcg atatcatcac ctatcgcaac ggtaaaaaag ttgaagtgat
    12361 gaacactgat gccgaagggc gtctggtgct tgccgatggt ctgattgatg ccagtgcgca
    12421 gaaaccggaa atgatcattg atgcggcgac cctcaccggg gcggcgaaaa ctgcgctggg
    12481 taatgattat cacgcgctgt tcagttttga cgatgcgctt gccggtcgtt tgctggcgag
    12541 tgcctcacaa gagaacgaac cattctggcg tctgccgctg gcggaattcc accgcagcca
    12601 gctgccgtct aactttgccg aactgaacaa taccggaagc gcggcgtatc cggcaggcgc
    12661 gagcacggcg gcgggcttcc tgtcgcactt tgttgagaac tatcagcaag gctggctgca
    12721 tatcgactgc tcggcgactt accgtaaagc gccggttgaa cagtggtctg cgggcgctac
    12781 gggacttggt gtgcgcacga tagctaatct gttaacggcg taaactaata tgccggatgc
    12841 ggtgtaaacg ccttatccgg cctacaaatt ctgcaaattc aacaaattgc gaatcccttg
    12901 taggcctgat aagcgtagcg catcaggcat ttgcgccccg aacgttgaat gaattgtggt
    12961 tcatatgtcc gaaacaaaaa acgaacttga agacctgctg gaaaaagcag caaccgaacc
    13021 ggcgcaccgc ccggcctttt tccgtactct actggaatcc accgtctggg tgcctggtac
    13081 ggcggcgcag ggcgaggctg tggttgaaga tagtgcgctt gatttacagc actgggaaaa
    13141 agaagacggc accagcgtca ttcctttttt cacctcgtta gaagcgcttc aacaggcggt
    13201 tgaagacgaa caggcatttg tcgtaatgcc cgttcgcaca ttgtttgaga tgacccttgg
    13261 cgaaacgctc ttccttaatg ccaaactgcc gaccggtaaa gaatttatgc cgcgtgaaat
    13321 cagtttgttg attggtggag agggaaatcc gctgagcagc caggaaatcc tggaaggcgg
    13381 tgaatcgctg atattatcgg aagtcgcaga gccgccagca caaatgattg attcactcac
    13441 caccttattt aaaaccatta agccggtgaa gcgtgctttt atttgttcaa ttaaagagaa
    13501 cgaagaggca cagcctaatt tacttattgg cattgaagcc gatggtgata tcgaagaaat
    13561 tattcaggcg acgggaagtg tagcgaccga tacattacct agcgatgaac caatcgatat
    13621 ttgtcaggtg aaaaaagggg aaaaaggaat tagccacttt attaccgaac atattgcgcc
    13681 attctatgaa cgtcgctggg gtggtttttt gcgtgacttt aaacagaacc ggataatcta
    13741 aaatatgccg ccctaaaggg cggcattcgg gcttacttgc tgctctgaag gttgttaacc
    13801 accagtaaat tgtcgccgga gagactccgg ttattattta cttgggaaaa cagcgcacca
    13861 gaaacaataa gtacgataca gaagcaccgt atcttcattg tgcggtccat gcgaagaccg
    13921 catcacgaca gcaccactgg cgatattgcc gcgatagcaa gcgctgagac gcgcttgctc
    13981 taacggtcgc ggaacatcct ttccttaagc cagcgcccgc ttgcgagacg gcggaaaggg
    14041 ccgcagtgga taatacacta acagaacaat aataactctc ttttgcttga caaaaagaga
    14101 gttactggcg agtattgtct ccgctgagac gcgcttgcat tactacggta atacaacctg
    14161 ctggaaaggc cgcgaaccag accagcaata aaaaaccgcc aaattcggcg gttttttatt
    14221 gcctgtaaaa tacttatttc actggctcaa ccggtaaatc tgcccgcgcg ccccattcac
    14281 tccatgcgcc gtcgtacagt ttcacgtttg gcacatccag cgtcgcgagt gctaacaaaa
    14341 ccacggctgc cgttacacca gagccgcagc tgacgataat tggtttgtcg tagctgacgc
    14401 cgcgaccaaa aaatatcgca tccagttcat cggtcgtttt tagttcgcct tcgcgcacca
    14461 gttccgtcca cggaacattc agtgctccgg gaatatgtcc gcgacgtaaa cctgggcgag
    14521 gttcatcaac ttctgcgtta aaacgtgcag ccgggcgggc atcaataatt tgcgccgtat
    14581 tttcatggct tgccaataat acatcggtta ctttcaccac ggcttcagga ttaaacgcgg
    14641 cgttaaactc tccttccggc agctctactg caccttcttc taacagcaga tcatcgcgct
    14701 gccagcctgc aagtccaccc cccagaatcg acactttctc tacaccaaag gtgcgcagca
    14761 tccaccatgc tcgtggggct gagaaaagat taccttcgtc atagacaatc aggtgcttat
    14821 cctggttaac gcctaattca cgcatcgcca cggcgaacgt ttccgggcgc ggcagcatgt
    14881 gcggaagcgg ggaagtgtga tcagaaagcg cttcgatatc aaaaaacact gcgccgggaa
    14941 tatgtccatt cagatactcc tgagcaacgt tacgatcctc ctgtccaggc gacgccatgc
    15001 gggcatcgat aatctgaatt tccgggtcat caatatgttc ggcgagccag tcggctccta
    15061 caaaccatgt cgtggacata ggcatctcca tttttgccgg ggttaggcta aattgtcgga
    15121 tgtttagtga aaagcgacaa gcaaaagagg ctgatttggc tgtctgagcc acaattctca
    15181 ctaaaattcg gattgtatgg gcgtgatact gaattacata acgtaaattc tcacgcataa
    15241 taattattcg acgttgctaa cgggccattg tggcagggat gaaaaatgaa aaagttacgc
    15301 gtagccgcct gcatgctaat gctggcgctg gcagggtgcg acaacaacga taacgcgcca
    15361 acagcggtga aaaaagatgc gccttctgaa gttactaaag cggcctcttc agaaaacgcg
    15421 agttcagcaa aactctccgt gccggagaga caaaaactgg cccaacagag tgccggtaag
    15481 gtgctgacat tgctggatct ctctgaagtc caacttgatg gtgcagccac gctggtgctg
    15541 acgttctcca tccctctcga cccggatcag gatttctcac gcgttattca tgtcgtcgat
    15601 aaaaaaagcg gcaaagtgga tggtgcctgg gagctgtcag ataatcttaa agagttacgt
    15661 ttacgccacc tcgaaccgaa acgtgatttg atcgttacta ttggcaagga ggtcaaagca
    15721 ctcaacaacg caaccttcag taaagattac gaaaaaacta taactacccg cgacatccaa
    15781 cccagcgtcg gttttgccag ccgtggttcg ctgctgcctg gcaaagtcgt tgaagggctg
    15841 ccggtaatgg cgctcaacgt taataatgtc gatgttaact tcttccgcgt taagccagaa
    15901 tctctgccag cattcattag ccaatgggaa taccgcaatt cgctggcgaa ctggcagtca
    15961 gacaaactgc tgcagatggc ggatctggtc tacaccggac ggtttgatct caatcctgcg
    16021 cgtaacaccc gtgaaaaatt attgctgccg ctgggcgata tcaaaccgct tcagcaggcg
    16081 ggcgtgtatc tggctgtgat gaatcaggct ggacgttacg attacagtaa tcccgcgacg
    16141 ctgtttacgt taagtgatat cggcgtttca gctcaccgtt atcacaatcg tctggatatc
    16201 tttacccaaa gtctggaaaa cggcgcggcc cagcaaggaa ttgaagtctc tttattaaat
    16261 gagaaagggc tgactctgac tcaggcaacc agtgacgctc aggggcatgt gcagctggaa
    16321 aatgataaaa acgcggcatt actgttggcg cgtaaagacg gtcagacaac gctactcgat
    16381 ttaaaacttc cggcgctgga cttagcagaa tttaacattg ctggcgcgcc aggctatagc
    16441 aaacagtttt tcatgtttgg cccacgcgat ctttatcgcc caggtgaaac ggtaatcctc
    16501 aatggtttgc tgcgtgatgc agacggtaaa gcgttgccca atcaacccat caagttagac
    16561 gtgattaaac ccgatgggca ggtactcagg agcgtcgtta gtcagccgga gaatggcctc
    16621 taccacttta cctggccact cgatagcaat gcggcaaccg gtatgtggca tattcgcgct
    16681 aacacgggcg ataaccagta tcggatgtgg gatttccacg tcgaagattt tatgccagag
    16741 cgcatggcgc tgaatctgac cggtgagaaa accccgctaa cgccgaaaga tgaagtgaaa
    16801 ttctccgtgg tggggtacta cctgtatggt gcacctgcta atggtaatac tttgcaaggg
    16861 caacttttcc tgcgcccact gcgtgaagct gtgtcagcct tacctggttt tgaattcggc
    16921 gatatagctg ccgaaaatct ttcccgcacg ctggatgaag ttcagttgac gctggatgat
    16981 aaagggcgcg gcgaagtttc tacagaaagc cagtggaagg aaacgcattc cccattacag
    17041 gttattttcc agggtagttt gctggaatcg ggcggtcgcc cggtgacgcg ccgcgctgag
    17101 caggctatct ggcctgccga tgcattgccg gggatccgtc cgcagttcgc ctcgaaatcg
    17161 gtttacgatt atcgcactga cagcacggtg aaacagccca ttgttgatga aggcagtaac
    17221 gccggttttg acatcgttta tagcgatgcg caaggcgtga aaaaagccgt gtcgggcttg
    17281 caggtgcgcc tgattcgcga acgccgcgat tactactgga actggtcaga agatgaaggc
    17341 tggcagtcac agtttgatca aaaagatctg atcgaaaatg aacaaactct ggatcttcag
    17401 gcggatgaaa ccggtaaggt cagtttcccg gtagagtggg gcgcttatcg tctggaagtc
    17461 aaagcgccga atgaagcggt cagtagcgtg cgtttctggg ctggctatag ctggcaggac
    17521 aacagcgacg ggagcggtgc cgtgcgaccc gaccgtgtca cgctgaaact ggataaagcc
    17581 agttatcgcc ctggcgatac cattaagttg catattgccg cgccaacggc gggtaaaggt
    17641 tatgcgatgg tcgagtccag tgaagggccg ttgtggtggc aagagattga tgttccggct
    17701 caagggctgg atctgacgat tccggtcgat aaaacctgga atcgtcatga tctgtattta
    17761 agtacgctgg tagtacgtcc tggcgataaa tctcgctccg cgacgccaaa acgcgcagtt
    17821 ggggtgttgc atctgccgct tggcgatgaa aaccgtcgcc tcgatctggc gctggaaaca
    17881 ccagcaaaaa tgcggccgaa tcaaccatta accgtgaaaa ttaaagccag cactaaaaat
    17941 ggcgagaagc ctaaacaggt gaatgtgctg gtgtctgccg ttgatagtgg tgtgctgaat
    18001 attactgact acgtcacgcc agatccgtgg caggcgttct ttggtcagaa acgctatggc
    18061 gcagacattt acgatattta cggtcaggtt attgaaggtc aggggcgtct ggcagctctg
    18121 cgtttcggtg gcgatggtga tgagctgaaa cgtggtggta aaccgccggt caatcacgtc
    18181 aatattgtcg cgcagcaggc gctgccggta acgctcaacg aacagggcga aggctcggtt
    18241 acactgccga ttggcgattt taacggtgaa ttacgcgtca tggcgcaagc ctggacggca
    18301 gatgacttcg gtagcaacga aagcaaagtg atagttgccg caccggtgat tgctgaactg
    18361 aacatgccgc gctttatggc gagtggcgat acctcgcgtc tgacgctgga tatcactaat
    18421 cttaccgata aaccgcaaaa actgaacgtt gccctgaccg ccagtggttt gcttgaactg
    18481 gtcagcgatt cacccgcagc cgttgaatta gcgccaggtg tgcgtactac gctgtttatc
    18541 ccggtgcgag cattgccagg ttatggcgat ggagaaattc aggccaccat tagcgggtta
    18601 gcgttaccgg atgaaaccgt tgccgatcag cagaagcagt ggaaaatcgg cgtccgtccg
    18661 gcgttcccgg cacaaacggt taattacggt acggcgttac agcctggtga gacatgggcg
    18721 attccggcgg atggattgca aaacttctcg cctgttacgc tggaagggca attgttgttg
    18781 agcggcaaac caccgctgaa cattgcacgt tatatcaaag agttaaaagc gtatccgtac
    18841 ggctgtcttg agcaaaccgc cagcggcctg ttcccgtcac tttataccaa cgcagcccaa
    18901 ctgcaggcgt tgggcatcaa aggcgacagt gatgagaaac gccgtgcatc ggtcgatatc
    18961 ggcatttccc gtttgctgca aatgcaacgt gataacggcg gctttgcgct gtgggataaa
    19021 aacggtgacg aagagtactg gctgacggct tacgtgatgg atttcctggt ccgcgcaggc
    19081 gaacagggtt acagcgtgcc gacagacgcc attaaccggg gtaat
//
LOCUS       DAMZET010000079        18975 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.079, whole genome shotgun sequence.
ACCESSION   DAMZET010000079 DAMZET010000000
VERSION     DAMZET010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.079"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(195..1553)
                     /gene="plaP"
                     /locus_tag="POB34_002778"
     CDS             complement(195..1553)
                     /gene="plaP"
                     /locus_tag="POB34_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HDI5919688.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1543..1605)
                     /gene="yoeI"
                     /locus_tag="POB34_002779"
     CDS             complement(1543..1605)
                     /gene="yoeI"
                     /locus_tag="POB34_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HDI5919689.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1820..2749)
                     /locus_tag="POB34_002780"
     CDS             complement(1820..2749)
                     /locus_tag="POB34_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI5919690.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2795..3619)
                     /locus_tag="POB34_002781"
     CDS             complement(2795..3619)
                     /locus_tag="POB34_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI5919691.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDVDALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3702..3956)
                     /gene="yoeB"
                     /locus_tag="POB34_002782"
     CDS             complement(3702..3956)
                     /gene="yoeB"
                     /locus_tag="POB34_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HDI5919692.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(3953..4204)
                     /gene="yefM"
                     /locus_tag="POB34_002783"
     CDS             complement(3953..4204)
                     /gene="yefM"
                     /locus_tag="POB34_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HDI5919693.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            4488..4538
                     /gene="hisL"
                     /locus_tag="POB34_002784"
     CDS             4488..4538
                     /gene="hisL"
                     /locus_tag="POB34_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HDI5919694.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    4514..4637
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4684..5583
                     /gene="hisG"
                     /locus_tag="POB34_002785"
     CDS             4684..5583
                     /gene="hisG"
                     /locus_tag="POB34_002785"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDI5919695.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5589..6893
                     /gene="hisD"
                     /locus_tag="POB34_002786"
     CDS             5589..6893
                     /gene="hisD"
                     /locus_tag="POB34_002786"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDI5919696.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALSASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLASAERLTAHKNAVTLRVNALKEQA"
     gene            6890..7960
                     /gene="hisC"
                     /locus_tag="POB34_002787"
     CDS             6890..7960
                     /gene="hisC"
                     /locus_tag="POB34_002787"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDI5919697.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7960..9027
                     /gene="hisB"
                     /locus_tag="POB34_002788"
     CDS             7960..9027
                     /gene="hisB"
                     /locus_tag="POB34_002788"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HDI5919698.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLLYDREILSWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGDSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLNLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            9027..9617
                     /gene="hisH"
                     /locus_tag="POB34_002789"
     CDS             9027..9617
                     /gene="hisH"
                     /locus_tag="POB34_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDI5919699.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNSWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9617..10354
                     /gene="hisA"
                     /locus_tag="POB34_002790"
     CDS             9617..10354
                     /gene="hisA"
                     /locus_tag="POB34_002790"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDI5919700.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYATQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADTLVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10336..11112
                     /gene="hisF"
                     /locus_tag="POB34_002791"
     CDS             10336..11112
                     /gene="hisF"
                     /locus_tag="POB34_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDI5919701.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            11106..11717
                     /locus_tag="POB34_002792"
     CDS             11106..11717
                     /locus_tag="POB34_002792"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDI5919702.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(11814..12791)
                     /gene="wzzB"
                     /locus_tag="POB34_002793"
     CDS             complement(11814..12791)
                     /gene="wzzB"
                     /locus_tag="POB34_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HDI5919703.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQTAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(12937..14103)
                     /gene="ugd"
                     /locus_tag="POB34_002794"
     CDS             complement(12937..14103)
                     /gene="ugd"
                     /locus_tag="POB34_002794"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HDI5919704.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GESSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(14352..15758)
                     /gene="gndA"
                     /locus_tag="POB34_002795"
     CDS             complement(14352..15758)
                     /gene="gndA"
                     /locus_tag="POB34_002795"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDI5919705.1"
                     /translation="MLKQQIGVVGMAVMGRNLALNIESRGYTVSIYNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSSEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDKGEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(15821..17236)
                     /gene="wzx"
                     /locus_tag="POB34_002796"
     CDS             complement(15821..17236)
                     /gene="wzx"
                     /locus_tag="POB34_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O78 family O-antigen flippase"
                     /protein_id="HDI5919706.1"
                     /translation="MRQHRKLVSYAVIITFLFIPIQVILSLIFNYYLAKNVALDVLNA
                     WYMFFALISLFSLLEFSYPILAINFFNSYQGNCKGALSFFIKKSLGVVIPLQIIFVVI
                     YSLFVNPLFIYFGMGLVVRSISNIINACVYAKQNIIIDKVYRFLYAIIMPTAFLFLFY
                     HSDKNIDLYDLTLVWFLSSLFCLIYSLSFLLLKHKKSVNRSEKSFHLPFKYNLKLFFT
                     VFPAIFIYTLSIYYLKIFGSESSSSSTIIYGFFLQIFNVYNLIIILVASYLMPTLSKR
                     FHDGVDVIPLTLKLLDISVLISVISTSFVALLGIPVAEYILKGKIDVISYYYIVAIII
                     IFYIETCQVILTSIGTAIGIYDFHRQSIISAVMVLILSCFLIPHYNAEGLMYSIIISQ
                     FFTCFIYNPKRVINKIGLDKVNYIKRLMVHFLFMLTLIFMGLYFQNDDILPRVVILSF
                     ITLIGVCFLYPNVKKIIVDFL"
     gene            complement(17233..17967)
                     /locus_tag="POB34_002797"
     CDS             complement(17233..17967)
                     /locus_tag="POB34_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HDI5919707.1"
                     /translation="MTTIVIPMAGQSSRFYKAGYDVPKYKLKINNKSVFFHSLRSFLK
                     FKDHFFLIIGIKGILDEKFVQYEMAELGINDFEIVLLEQQTNGQAETVVKGVKNCKRD
                     ISDILIFNIDTFRREINLPDNFSLKDCSGYLETFLGSGENWSNIMPESDVPNQVKMTA
                     EKQNISEYCCTGLYYFGDYKLLNIAYNNWLENEDTTKEVYIAPLYNHLITCGHKIFYT
                     VINRCDVVFCGVPNEYEKIKNGFDWK"
     gene            complement(17964..>18975)
                     /locus_tag="POB34_002798"
     CDS             complement(17964..>18975)
                     /locus_tag="POB34_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919708.1"
                     /translation="YDFGHLNSFFTSRTSITTEREFNSLQINNFCVIKKSKKKAKMLG
                     EANWFLSLPPALSIFTPRVFDVREDNDYAEYTIEYLYNLPLSDMAVFCELPKSCWVNV
                     FNSCRDFLNRSKEYSNSKKTLDMSELASYDKLYYDKTLKRLHDFELTTNIQLTKDVIF
                     NGKRFPSIKKIAEISSRFINPTKSDDVIITHGDFCFSNILYDFRSQRIKLIDPRGINY
                     NNELSIWGDIRYDLAKLCHSVIGCYDLIIAQKFLLEYDYVDNRIEFELYDDNYSDIVN
                     SFLGVFNSFEEFGYTIEEIQCITIHLFLSMLPLHSDSKLRQEAFIANAYRLFSLLNLE
                     YL"
BASE COUNT         5274 a         4582 c         4341 g         4778 t
ORIGIN      
        1 atatcattga aaacataaat taattaacca gatgaatgtt aatagggaaa ttattcatga
       61 ctggtggaat gcagaccaat aaccaaattc tctaaattag aacaaatggt tattaatgag
      121 gatgtcgata aaaaagaata aaaaaaccgg agccaaatat tcattcgact ccggtttatt
      181 attacaaaag atgattacgc tacgtcttcg tactgcggaa ccgggttacg gaaactcttg
      241 gtgacgcaag ccaggtaaat cagaccgata gccgcccaga tcagacccag aaccattgag
      301 ctttcttcca ggttaaccca cagcgcacca acggtcagcg caccacacat cggcaggaac
      361 agatactgga agtgatcttt cagcgtctta ttacgcttct cacggatcca gaactgcgag
      421 atgaccgaca ggttaacgaa ggtgaacgcc accaacgcac caaagttaat cagcgctgta
      481 gccattacca ggtcgaagtt gattgccagc agggcaatcg cgccaaccag gatgatgttc
      541 atcgccggag tacgccattt cgggtgtaca taaccgaaga agcttttcgg gaatacgccg
      601 tcacgaccca ttacgtacat cagacgcgct acgcctgcat gcgccgccat accggacgcc
      661 agtacggtaa tggtggagaa gatcagcgcg ccaacctgga acgctttacc cgcaacgtac
      721 agcatgattt caggctgtga cgcatccgga tctttaaagc gagagatatc cgggaagtac
      781 agctgcagga agtaagttgc aaagatgaag atcatgccgc caatcagcgc ggtcaggaaa
      841 atcgcacgcg ggatcacgcg ctctgcatct ttggtttctt ccgacaggtt gctgatgccg
      901 tcaaagccgg taaaggagaa gcacaggatt gtcgccccgg taatcatcgg gataacatgc
      961 gcatcgccag accagaacgg acgagtgctc gccagcgtac cagcgccttc accttcaaat
     1021 acgccataaa caaccatgcc cagaatcacc gcgatcagca ctacctgcaa cacgacgatt
     1081 acagtgttga agttcgctac ggatttcaga ctacgcaggt taaaggcggt catgaaggcc
     1141 accaacgcca ccacaaacat ccacgatggg atggaaggca ccagagcttc aaaatagatt
     1201 ttcgccagca gaatgttgat catcggcgcg aacagatagt cgagcagaga agaccaaccc
     1261 accataaagc cgacagtcgg gctaatggat ttctgggcgt aagtgtatgc agagccagca
     1321 gaaggatagc ggcgaaccag cttcccgtag ctcagagccg taaacaggat cgcaatcaat
     1381 gcgaacgcat aggctgtcgg cacatgacca tccgtgaggc ctgaaacgat accaaaagta
     1441 tcaaacagcg tcatcggctg catataggca agacccatca ttacaaccgg aactaacgta
     1501 agcgttttac gcaattccac gcgagaggtg tttggagtaa cgttatgcga catggtcatt
     1561 ctcctttacg gtgataaccg tcgcgtaagc aaaaaattgc cccatttttt tggattcctc
     1621 agcgacaaca actgtcgatt tttagtaaat atctatccgg tacgaagccc ggcctcttgg
     1681 tatgaatgat tggtttgaag caaaaaataa ccgacgctga tgaaacgtcg gtttttagtc
     1741 attttttgac agcggcgcat tgtgcctaaa tgggggggaa atgacaagag aatgagaggc
     1801 ttgtcataat aatttttctt taaatggctg attttccgtc atcagatttt gcataaacac
     1861 cggcgatcgc actatttgct aaaatttcat cccgccacca gccactggca agtcctgcgg
     1921 tctgttcatt ccagccaatc cagacgggtt caaagagagt ttgggcgacc actttcttct
     1981 cgattaacgc gccactatcg agaaaacgtt gcgccagata acggggtaaa tagccgcagc
     2041 cgaggccgct aatttgcagc tccagcttgg ttttaaaatc gaaaacggta atggcttctt
     2101 gttcgtcgag aagttgtgag gccgtagaag cgcctgcctg cgcagtatcc cccaccacaa
     2161 tcgcccgata tcgcttaata atacgacgat ttaacggctc ttcttccagg gctaacggat
     2221 gatgaggcgc gaccgcaaag acttgttcca gatcgcccaa ccgtgaaaaa ccaaactcac
     2281 tggaagatgg tggctcatgc atcgcgccaa caataatatc cgctcgcccc tgagtcaggg
     2341 catcccagga accaccgagt acgccattga taaacttcag gcgcgtcacg ctatgatgtt
     2401 gataaaaagc ttcaatcagt ggcgcaagca gagaaaaagg aaacgtgtcg tccacgccaa
     2461 tcaccagctc gttttcccag ccttcatgca gtttgatcgc ctgcttttcc agttctcgca
     2521 cggtatgcag aacttcccgc cctttctcta ataacatttt tccggtgcgg gtgaatttag
     2581 cgcggtgtcc gctacgatca agcaattgaa tattaaggtc gctttccagc ttgtgaacgg
     2641 tgtaactcag agcggacggc gtcttataga gtttggccga cgccgccgca aaactgcctt
     2701 ctttttctaa ggcatcgagg atcatcaaca catccagcag tggtttcata cacgccccct
     2761 tgcagtgcat gcggcgtcct gctggcgtga tcgtttactc cagcggcatt accagcggat
     2821 cgggatactg gtattcaaat cccagttcat tacaaatccg actgccatca ataatcttac
     2881 ctttgccgct gtccagacta tttctgaatt gcggaggttc cagccccagt aaacgggcca
     2941 tctgcggata gaaaacatta cgcgcagggt gagctggcgc acatatatta tagatgtgtc
     3001 cgcctttagg tgcctgtaac aacagagtga tagcgccaat aacatcttct aaatggacta
     3061 aattaacacc atgttcacca tcaggcgcgg tttttccggc aaagaagcgt ccgggatgac
     3121 gtcccggtcc gaccaggccc gcaagacgca gaatatcgac cgaagtaccg ggtaaattgt
     3181 gcagccagtc ttcgagttct tctaatactc gtccactatt ggttaccgga ttacgcgggg
     3241 tagtttcttt gaccgtgcct tgcgcgtcgc catagacaga tgttgagctg gtaaaaataa
     3301 ttcggggaat acgatgggcc agcgcgctat ccactaactc ttgtaccgct tgtaaataga
     3361 actcatcgcc ggggccgcta cgacgtgccg gaagcgtaat gaccagcgca tcgacatcca
     3421 tcagggcatc cagatcgtca gaatcgcaaa ctaactcagg ctccatgcga agcagatagc
     3481 tatcaatgcc actcattcgg gccgcttcga caccatcttg tgtggtttta ctcccggtga
     3541 cttgccagcc tcgcgctgaa agtgacatcg ccagcggcat gcctaaccac cctaacccga
     3601 caattgcgac ctttttcatc cgttatctcc tgatacacct tagatctata aggctacgct
     3661 agcgtatcaa aactgacaat tcattctatg aatgaatctg ttcaataatg ataacgacat
     3721 gctgcaatga gcagtgaatc gtcggtaacc gcgtatacca gacggtgctc ctctgtaatg
     3781 cgtcgggacc agaaacctga caaattatgt ttcaggggtt ctggcttccc cttaccttca
     3841 aatggcgttc tgcgggtatc tttgataagt tcattgatct ttttaacaat tcgcttatct
     3901 gtttcctgcc agtacagata atcatcccat gattcctcag accagattag tttcactcaa
     3961 tgatgtcctt ttccgttcct ttgcctgatt tcaggctatc gattgagtcc atcaatctcc
     4021 gggcgttagc gggggaacgc agtagataag ccgtctcttc cagggagtta tattcttcga
     4081 gtgacatcag aacacaagcc tctccattct gacgagtaat aaggatcggg gcatgatctt
     4141 caacggcttt catcattgtt gccgacaaat tctgacgcgc ttcgctgtag ctaattgtac
     4201 gcatgtcaat ctcctctttt gtacagttca ttgtacaatg atgggcgtta attaactatt
     4261 tattaattag tttgtagatc aagatattgt cagtgagacg aaaaatccag gcttcgctat
     4321 ttttggtgcc gtcagcttag aggacagtcc tcttagcgcc ctcctttccc cgctcattca
     4381 ttaaacaaat ccattgccat aaaatatata aaaaagccct tgctttctaa cgtgaaagtg
     4441 gtttaggtta aaagacatca gttgaataaa cattcacaga gacttttatg acacgcgttc
     4501 aatttaaaca ccaccatcat caccatcatc ctgactagtc tttcaggcga tgtgtgctgg
     4561 aagacattca gatcttccag tggtgcatga acgcatgaga aagcccccgg aagatcacct
     4621 tccgggggct tttttattgc gcgattgata acggttcaga caggtttaaa gaggaataag
     4681 aaaatgacag acaacactcg tttacgcata gctatgcaga aatccggccg tttaagtgat
     4741 gactcacgtg aattgctggc gcgctgtggc attaaaatca atcttcacac ccagcgcctg
     4801 atcgcgatgg cagaaaacat gccgattgat attctgcgcg tgcgtgacga cgacattccc
     4861 ggtctggtaa tggacggcgt ggtagacctt ggaattatcg gcgaaaacgt gctggaagaa
     4921 gaactgctta accgccgcgc ccagggtgaa gatccgcgct actttaccct gcgtcgactg
     4981 gatttcggcg gctgccgact ttcactggca acgccggttg atgaagcctg ggacggcccg
     5041 ctctccttaa atggtaaacg tatcgccacc tcttatcctc acctgctcaa gcgttatctc
     5101 gaccagaaag gcatctcttt taaatcctgc ttactgaacg gttctgttga agtcgccccg
     5161 cgtgccggac tggcggatgc gatttgcgat ctggtttcca ccggtgccac gctggaagca
     5221 aacggcctgc gtgaagtcga agttatctac cgctcgaaag cctgcctgat ccagcgcgat
     5281 ggcgaaatgg aagaatccaa acagcaactg atcgacaaac tgctgacccg tattcagggt
     5341 gtgatccagg cgcgcgaatc aaaatacatc atgatgcacg caccgaccga acgtctggat
     5401 gaagtcatcg ccctgctgcc aggtgccgaa cgcccaacca ttctgccgct ggcgggtgac
     5461 caacaacgcg tagcgatgca catggtcagt agcgaaaccc tgttctggga aacgatggaa
     5521 aaactgaaag cgctgggtgc cagttcaatc ctggtcctgc cgattgagaa gatgatggag
     5581 tgatcgccat gagctttaac acaatcattg actggaatag ctgtactgcg gagcaacaac
     5641 gccagctatt aatgcgcccg gcgatctccg cttctgaaag cattacccgc actgttaacg
     5701 atattctcga taacgtgaaa gcacgcggcg atgaggccct gcgggaatac agcgcgaagt
     5761 ttgataaaac cacggttacc gcgctgaagg tgtctgcaga ggaaattgcc gccgccagcg
     5821 aacgcctgag cgacgagcta aaacaggcga tggcggtggc agtaaagaat attgaaacct
     5881 tccacactgc gcaaaaactg ccgccggtag atgtagaaac tcagccaggc gtacgttgtc
     5941 agcaagtcac gcgcccggtg gcttcggtag gtttgtatat tcctggcggc tccgccccgc
     6001 tcttctcaac ggtattaatg ttggcaactc cggcgcgtat tgcgggctgt aaaaaagtgg
     6061 tgctgtgctc accgccgccg attgccgatg agatccttta tgcggcgcag ctgtgcggtg
     6121 tacaggacgt gtttaacgtc ggcggcgcac aggccattgc cgcactggcg tttggtactg
     6181 aatctgtgcc aaaagtggac aaaatcttcg ggccaggcaa cgcctttgtc accgaggcaa
     6241 aacgccaggt aagccagcgt ctggacggtg cggcgatcga tatgcccgca ggcccgtcgg
     6301 aagtgctggt gattgccgac agcggcgcta cgccagattt cgtggcttct gatttgcttt
     6361 ctcaggctga acacggcccg gactctcagg tgattttact gacgcccgat gctgatatgg
     6421 cgcgtcgcgt tgccgaagct gtcgaacgcc agctggcaga actgccgcgt gccgaaaccg
     6481 cacgtcaggc actgagcgcc agccgcctga tcgtgactaa agatttagcg cagtgcgtag
     6541 agatttccaa ccagtacggc ccggagcacc tgatcattca gacccgcaac gcccgcgaac
     6601 tggtcgatgg catcaccagc gccggttcgg tatttcttgg tgactggtca ccggaatcgg
     6661 caggcgacta tgcctccggc actaaccatg ttctaccgac ttacggttac accgccacct
     6721 gttccagcct cgggctggca gatttccaga agcgcatgac cgtgcaggaa ctctcgaaag
     6781 aaggcttttc cgcgctggct tcgaccattg aaacactggc ctccgccgag cgcctgaccg
     6841 cccataaaaa tgccgttact ttgcgtgtta acgcccttaa ggagcaagca tgagcaccgt
     6901 gactattacc gatttagcgc gtgaaaacgt ccgcaacctg acgccgtatc agtcagcgcg
     6961 tcgtctgggc ggtaacggcg atgtctggct gaacgccaac gaatacccca ctgccgtgga
     7021 gtttcagctt actcagcaaa cgctcaaccg ctacccggaa tgccagccga aagcggtgat
     7081 tgaaaattac gcgcaatatg caggcgtaaa accggagcag gtgctggtca gccgtggcgc
     7141 ggacgaaggt attgaactgc tgattcgcgc tttttgcgaa ccgggtaaag acgccatcct
     7201 ctactgcccg ccaacgtacg gcatgtacag cgtcagcgcc gaaacgattg gcgtcgagtg
     7261 ccgcacagtg ccgacgctgg acaactggca actggactta cagggcattt ccgacaagct
     7321 ggacggcgta aaagtggtct atgtttgcag ccccaacaac ccgaccgggc aactgatcaa
     7381 cccgcaggat tttcgcaccc tgctggagtt aacgcgcggt aaggcgattg tggttgccga
     7441 tgaagcctat atagagtttt gtccacaggc atcgctggct ggctggctgg cggaatatcc
     7501 gcacctggct attttgcgca cactgtcgaa agcttttgct ctggcggggc ttcgttgcgg
     7561 atttacgctg gcaaacgaag aggtcatcaa cctgttaatg aaagtgatcg ccccctaccc
     7621 gctctcgacg ccggttgccg acattgcggc ccaggcgtta agcccgcagg ggatcgtcgc
     7681 catgcgtgag cgggtggcgc aaattattgc agaacgcgaa tatctgattg ccgcattgaa
     7741 agagatcccc tgcgtggagc aggtttttga ctctgaaacc aactacattc tggcgcgctt
     7801 taaagcctcc agtgcagtgt ttaaatcttt gtgggatcag ggcattatct tacgtgatca
     7861 gaataaacaa ccctctttaa gcggctgcct gcgaattacc gtcggaaccc gtgaagaaag
     7921 ccagcgcgtc attgacgcct tacgtgcgga gcaagtttga tgagtcagaa gtatcttttt
     7981 atcgatcgcg atggaaccct gattagcgaa ccgccgagtg attttcaggt ggatcgtttt
     8041 gacaaactcg cctttgaacc gggcgtgatc ccggaactgc tgaagctgca aaaagcgggc
     8101 tacaaactgg tgatgatcac taatcaggat ggtcttggaa cacaaagttt cccgcaggcg
     8161 gatttcgatg gcccgcacaa cctgatgatg cagatcttca cctcgcaagg cgtgcagttt
     8221 gatgaagtgc tgatttgccc gcacctgccc gccgatgagt gcgactgccg taaaccgaaa
     8281 gtaaaactgg tggagcgtta tctcgctgag caagcgatgg atcgcgccaa cagttatgtg
     8341 attggcgatc gcgcgaccga cattcaactg gcggaaaaca tgggcattaa tggtttactc
     8401 tacgaccgcg aaatcctgag ctggccgatg attggcgagc agctcactaa acgagaccgt
     8461 tacgcccatg tagtgcgcaa caccaaagag acgcaaattg acgtccaggt gtggctggat
     8521 cgcgaaggtg acagcaagat taacaccggc gttggcttct ttgatcatat gctggatcag
     8581 atcgccaccc acggcggttt ccgcatggaa atcaacgtca aaggcgacct ctatatcgac
     8641 gatcaccaca ccgtcgaaga taccggcctg gcgctgggcg aagcgttaaa aattgccctc
     8701 ggtgacaaac gcggtatttg ccgctttggt tttgtgctgc cgatggacga atgccttgcc
     8761 cgttgcgcgc tggatatctc tggtcgcccg cacctggaat ataaagccga gtttacctac
     8821 cagcgcgtgg gcgatctcag caccgaaatg atcgagcact tcttccgttc tctctcttac
     8881 accatgggcg tgacgctaaa cctgaaaacc aaaggtaaaa acgatcatca ccgtgtagag
     8941 agcctgttca aagcctttgg tcggaccctg cgccaggcca tccgcgtgga aggcgacacc
     9001 ctgccctcgt cgaaaggagt gctgtaatga acgtggtgat ccttgatacc ggctgcgcca
     9061 atttgaactc agtgaagtct gccattgcgc gtcacggtta tgaacccaaa gtcagccgtg
     9121 acccggacgt cgtgttgctg gccgataaac tatttttacc cggcgttggc actgcacaag
     9181 cagcgatgga tcaggtgcgt gagcgcgagc tgtttgatct catcaaagcc tgtacccaac
     9241 cggtgctggg catctgctta gggatgcaac ttctggggcg gcgcagcgaa gagagcaacg
     9301 gcgtcgattt gctgggcatc atcgacgaag acgtgccgaa aatgaccgac tttggtctgc
     9361 ccctgccgca tatgggctgg aaccgcgttt acccacaggc aggcaaccgc ctgtttcagg
     9421 ggattgaaga cggcgcgtac ttttactttg ttcacagcta cgcaatgcca gtcaattcgt
     9481 ggaccatcgc ccagtgtaat tacggcgaac cgtttaccgc ggcggtacaa aaagataact
     9541 tctacggcgt gcagttccac ccggagcgtt ctggtgccgc tggtgctaag ttgctgaaaa
     9601 acttcctgga gatgtgatga ttattccggc attagattta atcgacggca ccgtggtgcg
     9661 tctccatcag ggcgattatg gcaaacaacg cgattacggt aacgacccgc tgccacgctt
     9721 acaggattac gccacacaag gtgctgaagt gctgcacctt gtggatctga ccggggcaaa
     9781 agatccggct aaacgtcaaa tcccgctgat taaaaccctg gtcgcgggcg ttaacgttcc
     9841 ggtgcaggtt ggcggcggcg tgcgtagcga agaagacgtg gcggcgttac tggaagcggg
     9901 cgttgcgcgc gtggtggttg gctccaccgc ggtgaaatca ccagaaatgg tgaaaggctg
     9961 gtttgaacgt ttcggtgccg ataccttagt actggcgctg gatgtccgta ttgacgagca
    10021 aggcaacaag caggtggcgg tcagcggctg gcaagagaac tcgggcgtgt cgctggaaca
    10081 actggtggaa acctatctgc ccgtcggcct gaaacatgtg ctgtgtaccg atatctcacg
    10141 cgacggtacg ctggcaggct ctaacgtctc tttatatgaa gaagtgtgcg ccagatatcc
    10201 gcaagtagca tttcagtcct ccggcggcat tggcgacatt aacgatgttg ctgccctgcg
    10261 tggaactggc gtgcgcggcg taattgtcgg tcgcgcatta ctagaaggta aattcaccgt
    10321 gaaggaggcc atcgcatgct ggcaaaacgc ataattccat gtctcgacgt tcgtgatggt
    10381 caggtggtga aaggcgtaca gtttcgcaac catgaaatca ttggcgatat cgtaccgctg
    10441 gcaaaacgct acgccgaaga aggtgcagac gaactggtgt tctacgatat caccgcttcc
    10501 agcgatggcc gtgtggtaga taaaagctgg gtatcgcgcg tggcggaagt gatcgacatt
    10561 ccgttctgcg tggcgggcgg gattaagtct ctggaagatg ccgcgaaaat tctttccttt
    10621 ggcgcggata aaatctccat taactcccct gcgctggcgg acccgacatt aatcactcgc
    10681 ctggcggatc gctttggcgt gcagtgtatt gtggtcggta ttgatacctg gtacgacgct
    10741 gaaaccggta aatatcatgt gaatcaatat accggcgatg aaagccgcac ccgcgtcact
    10801 cagtgggaaa cgctcgactg ggtacaggaa gtgcaaaaac gcggtgccgg agaaatcgtc
    10861 ctcaatatga tgaatcagga cggcgtgcgt aacggttacg atctcgaaca actgaaaaaa
    10921 gtgcgtgaag tttgccacgt cccgctgatt gcctccggtg gcgcgggcac catggaacac
    10981 ttcctcgaag ccttccgcga tgccgacgtt gacggcgcgc tggcagcttc cgtattccac
    11041 aaacaaataa ttaatattgg tgaattaaaa gcgtacctgg caacacaggg cgtggagatc
    11101 aggatatgtt aactgaacaa caacgtcgag aactggactg ggaaaaaacc gacggactta
    11161 tgccggtgat tgtgcaacac gcggtatccg gcgaagtgtt aatgctgggc tatatgaacc
    11221 cggaagcctt agacaaaacc atcgaaagcg gcaaagtcac cttcttctcg cgcactaaac
    11281 agcgactgtg gaccaaaggc gaaacatcgg gcaattttct caatgtggtg agtattgccc
    11341 cggactgcga caacgacacg ctactggtac tggcgaatcc catcggccca acctgccaca
    11401 aaggcaccag cagctgcttc ggcaacaccg ctcaccagtg gttgttcctg tatcaactgg
    11461 aacaactgct cgccgaacgt aaatctgccg acccggaaac ctcctacacc gccaaactgt
    11521 atgccagcgg caccaaacgc attgcgcaga aagtgggcga agaaggcgtg gaaaccgcac
    11581 tggcagcaac ggtacatgac cgctttgagc tgaccaacga ggcatctgac ttgatgtatc
    11641 acctgctggt attgttgcag gatcaggatc tggatttaac aacggtgatt gagaacctgc
    11701 ataaacggca tcagtgagtt gcggggtaag cggatgcgat attgttgtcg catccggcaa
    11761 aaaaaacggg caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa agattacttc
    11821 gcgttgtaat tacgcagagc gttacgcccc agcacaatcc ccgcgccaac catgccgccc
    11881 agcagcaccg ccagaatcaa ggtgattgcc tttttcggac tatcgcgacg aataggtaac
    11941 gtcggtttca tcacataacg gtaagcatga atatcaagat catcaacttt taagctttca
    12001 atatccagca ggttttgacg agtctgatag tagtttggcg agaacaccaa cggacgggtc
    12061 gcctcatgct taatcatcga ctccagcgct tcgctcccca aaaggaacaa cgtatcttgc
    12121 gtcacatctt cagtctgctg aacctgtggc tttgtcacct gcgcctgatt cgcatactgc
    12181 aacgcttcct gaatctgacg gatacgcaga tctttctgct cctgcgcaac cacttcctgg
    12241 gttcttaaag aatcctgcaa gtttttccgt cccagagcaa tgttgtcttt gagatccttt
    12301 tctagctctt gattcacttt atcatcaact tgctgaatgt attgggccaa cttcatttgt
    12361 gcgccctctg ctgtttgccc aacataagaa acagtcaatg gtaattgctg gttcttaaca
    12421 gaaggttcga tggtaagttt ctctggctct tcctgattat ccagcgtttc tgctaatgca
    12481 gagaaggcag aactgaagcg accaattaac gtctcctgca aatccgatac tttcggtgca
    12541 gtctgaccat agataacatt catggcattg ttatagccag caatttgccc cacgtcgggc
    12601 tgagtgataa ttgctgttga cgtccatttc tccttcgcta ctgccaaata tccaatagcc
    12661 agggcaatag ccacaatgac ggaaatgatg attgtcatct tgccacgcca caactgcact
    12721 agtaaatcaa tcaaatcaat ctgttccggg tcatggtttt gcccagaaac attattattt
    12781 tctactctca tcattaccct aactgacgga aatttccaag tccgcatagt ttagcgataa
    12841 ttctcgtgaa tgctatagga taagtgataa aaataatgaa ttattgagga agtaacaaaa
    12901 gacaatcagg gcgtaaatag ccctgataac aagatgttag tcactgccaa agagatcgcg
    12961 ggtgtatacc ttatctgcca catccttaag ctcttctgcc atacgattgg agataatgac
    13021 gtcagcttgt tgtttgaaag tggcgagatc tcgttccagg cgagagttga agaatgagtc
    13081 ttctttcatc accggttcgt aaatgatgac ttcaacgcct ttcgccttga tacgtttcat
    13141 aatcccctga atggaagacg cgcggaagtt gtctgaaccg ctcttcataa tcagacgata
    13201 aatacccacc acttgcggct tgcgtgacaa aatggcatcg gcaataaaat ctttacgcgt
    13261 gcggttagca tcgacaattg ccgaaatcag gttattcggc acagactgat aattcgccag
    13321 taactgtttg gtatctttcg gcaaacaata accaccataa ccaaacgacg gattgttgta
    13381 atggttgcca atacgcggat cgagacaaac gccttcgata atctggcgag tattcagacc
    13441 taaactttct gcatagctat ccagttcatt aaagtacgct acgcgcatcg ccagataggt
    13501 attagcgaaa agttttatcg cttctgcttc agtggagtcg gtaaacaggg tcgggatatt
    13561 ttgcttaatc gccccttcct gtaacaacgc agcaaaacgt tcagcgcgtt ctgaactctc
    13621 accgatgaca atacgtgaag gatggagatt atcgtaaagg gctttacctt cgcggagaaa
    13681 ttccggagag aaaataatat tttcagtacg atatttctta tgcatcgctg cggtaaaacc
    13741 aacgggaacc gttgatttga tgaccataac cgcataagga tttatctcaa ctacgtcttt
    13801 aattactgat tctacactgg atgtattgaa ataattagtt ttaggatcat agtcggttgg
    13861 agtggcgatg atgacataat cagcatcccg gtaggcttca tttttatcta atgtggcatt
    13921 aaagtgtatt ttatctgatt gcaaaaactg ctgaatttcc ttatcaacaa ttggagatat
    13981 ccgatcattc agcatagcaa cgcgtgacgg taaaatatct aatgccacaa cctcatgatt
    14041 ttgtgcgatt agaagcccgt ttgacaagcc tacatagccg gtaccggaaa tggtgatttt
    14101 catttcatgc tctcagaatt aacttaactg tgaatcatga tgtttttagc atcctgataa
    14161 gagctaaaag ttttaacgct acgttgtcag atggtcaacg caacggaaaa caattcagat
    14221 tttctttata agaatagctg atatttatta agttaatatt aagcaatgaa atttagtcta
    14281 taagataaat ggcaaaaaaa agcccggtgc aatatacgcc gggcctgaat tttattgttg
    14341 gttaaatcag attaatccag ccattcagta tggaacacac cttctttatc aatgcgctta
    14401 taagtatgtg caccgaaata gtcacgctgt gcctggatca ggttcgcagg cagaacagcg
    14461 gcacggtagc tatcgtaata ggcaaccgca gcggcgaagg tcggaaccgg gataccgttc
    14521 tgtactgcat aagcaacgac atcgcgcagc gcctgctggt agtcatcggc aatttgcttg
    14581 aagtacgggg ccagcagcag gttagcgatc tgcggatttt cggcatatgc atcggtgatt
    14641 ttctgcagga actgcgcacg gatgatgcag ccagcacgga aaatcttcgc gatttcgccg
    14701 tagttcagat cccagttgta ctcttcagac gctgcgcgca gctgagagaa accctgagcg
    14761 taagaaacga ttttgcctag atacagagca cgacgaactt tttcaatgaa ctcacctttg
    14821 tcgccagctg gctgcgcttg cgggcctgag agaactttag atgcggcaac acgctgctct
    14881 ttcagagaag agatataacg tgcaaacaca gactcggtaa tcagcgacag cggttcgccg
    14941 agatccagcg cgctctggct ggtccattta ccggtacctt tgttagccgc ttcatccagg
    15001 atcacatcaa ccaggtagtt accgtcttca tcttttttgg tgaagatgtc tttggtgatg
    15061 tcgatcaggt agctactcag ttcaccgtta ttccactcgg taaaggtctg tgccagttct
    15121 tcgttggtga ggttcaggcc acctttaagc agagaatagg cttcagcaat cagctgcata
    15181 tcgccgtatt caataccgtt gtgaaccatc ttcacatagt gacctgcacc atcggcacca
    15241 atataggtaa cgcatggctc cccgtcttca gctactgcgg cgattttggt caggatcggt
    15301 gcaaccagtt cataggcttc tttctggcca ccaggcatga tggacggccc tttcagcgcc
    15361 ccctcttcac cgccggaaac accggtaccg atgaagttaa agccttctga agaaagctca
    15421 cggttacgac gaatggtgtc ctggaagaag gtgttaccac catcaatgat gatgtcacct
    15481 ttatcgaggt acggcttgag ggaatcaata gcagcatccg tgcctgcacc tgctttcacc
    15541 attaacagga tgcgacgagg cgtttccaga gattcaacaa actctttcac cgtatagtaa
    15601 ggaaccagtt tcttgcctgg attttcggca atcacttctt ccgtcttttc acgggaacgg
    15661 ttgtaaatag agacggtata accacggctt tcgatgttga gcgcaaggtt gcgccccatc
    15721 acggccatac cgactacgcc gatctgttgc ttcagcattg ttaactcctt tctaagatag
    15781 aacaagattt tccatttaaa atgcaaataa aattaaaaaa ctacaagaaa tcaacaatta
    15841 tttttttcac atttgggtag agaaaacaaa caccaatcaa tgtgataaaa gaaagaatca
    15901 caactctcgg caatatatca tcattttgaa aatataaccc cataaaaata agagtcaaca
    15961 taaacaaaaa gtgcaccatt aatcttttaa tataattaac tttatctaat ccaattttat
    16021 ttataacacg tttagggtta taaataaagc atgtaaagaa ttgagatata atgatactat
    16081 acatcaaacc ctcagcattg taatgaggta ttaaaaaaca tgacagaatt aaaaccataa
    16141 ctgctgatat aatactttgc ctatgaaaat cgtaaatacc aatggcagtg ccaattgagg
    16201 ttagaataac ctgacatgtt tcaatataga atataataat tattgctact atataatagt
    16261 aggatattac atctattttc cccttaagaa tatactccgc aacaggaata cctaacaatg
    16321 ccacaaaaga agtagaaata acagatataa gtacactgat atctaatagt ttaagtgtga
    16381 gagggataac atcaacacca tcatggaaac gctttgagag cgtaggcatc aaataacttg
    16441 caaccaaaat aattatcaaa ttataaacat taaatatttg caaaaagaaa ccatatataa
    16501 tagtagaact agaactgctt tctgaaccaa atattttcaa ataatatata gacaatgtat
    16561 agatgaaaat tgcaggaaaa acagtaaaaa aaagcttaag gttatacttg aatggtagat
    16621 ggaatgattt ttcgctcctg ttgacagact tcttatgctt aaggagtaaa aatgacaaag
    16681 aatatattaa gcagaatagt gagctcaaaa accatacaag agttaaatca tacaaatcaa
    16741 tgtttttatc agaatgataa aacaagaaaa ggaaagcagt tggcataatt atggcgtaca
    16801 gaaatcgata aactttatcg ataataatat tttgttttgc atatacacat gcattaataa
    16861 tattcgatat actacgtacc accaaaccca taccaaaata aataaaaagc gggttaacaa
    16921 ataaactata aattacaaca aatatgatct gaaggggtat tactacccct aatgatttct
    16981 ttatgaagaa agacagagct cctttacaat ttccctgata gctgttaaaa aaattaattg
    17041 ctaatatggg ataggaaaat tccaacaaag aaaaaaggga aatgagtgca aaaaacatat
    17101 accaagcatt taaaacatcc agagcaacgt ttttagctaa ataatagtta aaaattaacg
    17161 acagaataac ttgtataggt ataaacaaaa aagtaataat cacagcataa ctgactaatt
    17221 ttctgtgctg tctcatttcc agtcaaaccc attctttatt ttttcatatt cattaggcac
    17281 accacagaac accacatcac atctgtttat aacggtataa aaaattttgt gcccacatgt
    17341 aatcaaatga ttatatagag gagcgatata cacctcttta gtagtatctt cattttctag
    17401 ccagttatta taagctatat tcaacaactt ataatcacca aaataatata aaccagtgca
    17461 acaatattca gatatatttt gtttttccgc agtcattttt acttgattag ggacatctga
    17521 ttcaggcata atattactcc aattctcacc tgaccctaaa aatgtttcca aatacccaga
    17581 gcaatctttc aaactaaaat tatcaggcaa attaatctct ctgcgaaaag tatctatatt
    17641 aaaaattaaa atgtcactaa tatctctctt gcaatttttt actcctttca caaccgtctc
    17701 tgcttgacca tttgtttgct gttcgagcaa aacaatttca aaatcattta tacctaattc
    17761 ggccatttca tattgaacaa acttttcatc tagtatgcct ttaataccta taattagaaa
    17821 aaaatggtcc ttaaatttta gaaaacttcg tagtgaatga aaaaatacgc ttttgttatt
    17881 aatttttaac ttatacttag gaacatcata acctgcttta taaaagcgtg atgattgtcc
    17941 tgccatagga attacaatcg ttgtcataaa tactccaaat ttaacaaact gaacagtcta
    18001 taagcatttg ctataaatgc ttcttgtcta agtttagaat cagaatgaag cggcaacatt
    18061 gataaaaata aatgtatggt tatacattgt atctcttcaa tagtataccc aaattcttca
    18121 aaagaattaa acacgcctaa aaatgaattc actatgtcgc tgtagttatc atcatatagt
    18181 tcaaattcaa ttctgttatc tacataatcg tattccagca aaaatttctg agcgataata
    18241 agatcataac atccaattac cgagtgacat aacttagcca agtcatacct tatatctccc
    18301 catatgctta actcattgtt atagttaata cctctgggat cgattaattt aattctctga
    18361 cttctaaaat cgtataatat attactaaag caaaaatcgc catgtgtaat tataacatca
    18421 tcactcttag ttgggttaat aaagcgtgat gatatttctg ctattttttt tatcgatgga
    18481 aatcttttac cattaaatat aacatcttta gtcaattgta tgtttgttgt caattcaaaa
    18541 tcatgtaacc tttttagggt cttatcatag tacaatttat catatgatgc cagttcagac
    18601 atatctagcg tttttttact attagagtat tcttttgatc tatttaagaa atctcgacac
    18661 gagttaaaca cattcaccca acatgactta ggtaactcac aaaacactgc catatcagac
    18721 aaaggaagat tataaagata ttctattgta tactctgcat aatcattatc ttctctaaca
    18781 tcaaaaaccc tcggagtgaa gatactcaat gcaggcggca aactcaaaaa ccaattcgct
    18841 tcgcctagca tttttgcttt cttcttactt ttttttatca cacaaaaatt atttatttga
    18901 agactattaa actcacgctc agtagtgata ctcgttcttg atgtaaaaaa gctgtttaaa
    18961 tgaccaaaat catac
//
LOCUS       DAMZET010000080        18906 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.080, whole genome shotgun sequence.
ACCESSION   DAMZET010000080 DAMZET010000000
VERSION     DAMZET010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.080"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..784)
                     /gene="mhpF"
                     /locus_tag="POB34_002799"
     CDS             complement(<1..784)
                     /gene="mhpF"
                     /locus_tag="POB34_002799"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HDI5919709.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPKSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPG"
     gene            complement(781..1590)
                     /gene="mhpD"
                     /locus_tag="POB34_002800"
     CDS             complement(781..1590)
                     /gene="mhpD"
                     /locus_tag="POB34_002800"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HDI5919710.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(1600..2466)
                     /gene="mhpC"
                     /locus_tag="POB34_002801"
     CDS             complement(1600..2466)
                     /gene="mhpC"
                     /locus_tag="POB34_002801"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HDI5919711.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLKWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(2484..3428)
                     /gene="mhpB"
                     /locus_tag="POB34_002802"
     CDS             complement(2484..3428)
                     /gene="mhpB"
                     /locus_tag="POB34_002802"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HDI5919712.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISTFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(3430..5094)
                     /locus_tag="POB34_002803"
     CDS             complement(3430..5094)
                     /locus_tag="POB34_002803"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HDI5919713.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQNDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTTGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            5171..6118
                     /gene="mhpR"
                     /locus_tag="POB34_002804"
     CDS             5171..6118
                     /gene="mhpR"
                     /locus_tag="POB34_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HDI5919714.1"
                     /translation="MIFYCALSIGRVFSATIKTCPNVHQVHNVVLTIEMSINMQNNEQ
                     TEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLHRTTVRRLLETLQEEGYVRRS
                     PSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLREVVWPTDVSTLDVDAMVVRE
                     TTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQERKELIEMLAARPGDDYQLAR
                     EPLKLQAILARARKEGYGQNYRGWDQEEKIASIAVPLRSEQRVIGCLNLVYMASAMTI
                     EQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHLR"
     gene            6195..7277
                     /gene="lacI"
                     /locus_tag="POB34_002805"
     CDS             6195..7277
                     /gene="lacI"
                     /locus_tag="POB34_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HDI5919715.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            7400..10474
                     /gene="lacZ"
                     /locus_tag="POB34_002806"
     CDS             7400..10474
                     /gene="lacZ"
                     /locus_tag="POB34_002806"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HDI5919716.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRVGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGSYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHIIPQ
                     LTTSETDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCSADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            10526..11779
                     /gene="lacY"
                     /locus_tag="POB34_002807"
     CDS             10526..11779
                     /gene="lacY"
                     /locus_tag="POB34_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HDI5919717.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTVNNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            11845..12456
                     /gene="lacA"
                     /locus_tag="POB34_002808"
     CDS             11845..12456
                     /gene="lacA"
                     /locus_tag="POB34_002808"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HDI5919718.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLLKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAAGVPCRVIREINDRDKQYYFKDYKVESSV"
     gene            complement(12559..13740)
                     /gene="cynX"
                     /locus_tag="POB34_002809"
     CDS             complement(12559..13740)
                     /gene="cynX"
                     /locus_tag="POB34_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HDI5919719.1"
                     /translation="MRNHQKTFTILLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAALTPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSTREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(13746..14216)
                     /gene="cynS"
                     /locus_tag="POB34_002810"
     CDS             complement(13746..14216)
                     /gene="cynS"
                     /locus_tag="POB34_002810"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HDI5919720.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(14247..14906)
                     /gene="cynT"
                     /locus_tag="POB34_002811"
     CDS             complement(14247..14906)
                     /gene="cynT"
                     /locus_tag="POB34_002811"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HDI5919721.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            15016..15915
                     /gene="cynR"
                     /locus_tag="POB34_002812"
     CDS             15016..15915
                     /gene="cynR"
                     /locus_tag="POB34_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HDI5919722.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            complement(15955..17238)
                     /gene="codA"
                     /locus_tag="POB34_002813"
     CDS             complement(15955..17238)
                     /gene="codA"
                     /locus_tag="POB34_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HDI5919723.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(17228..18487)
                     /gene="codB"
                     /locus_tag="POB34_002814"
     CDS             complement(17228..18487)
                     /gene="codB"
                     /locus_tag="POB34_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDI5919724.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
BASE COUNT         4462 a         5200 c         5012 g         4232 t
ORIGIN      
        1 cgcccggtaa attgaccggt ttatcctgcg ggataacttc aaactgcacg cgctgtttca
       61 ggcgataacc cggtacgtaa gcctgcaccg cctcagccat ttcattgatt gaggcttcga
      121 tatcatcttg tgaagcttcg tcgctcaata catacaccgt gtcacgcatc atcaacggtg
      181 gctctgctgg gttaagcaca ataatcgcct tcccttttgc cgcgccgccc accacttcaa
      241 tggctcggga agtggtttcc gtaaattcat cgatattggc acgcgtgcca ggtccggcag
      301 atttactggc gatagaagcg ataatttcgg cgtaatgaac acgcgccacg cgtgaaactg
      361 ccgccaccat tggaatggtg gcctggccgc cgcaggtgac catgttgacg ttcagttgat
      421 cgacgttcgc ctcgaggtta accaccggca cgcagtaagg gccgatggca gcaggcgtca
      481 ggtcaattaa gcgaatatcc ggtttcgctt cgcgtaaagc ggcatcgttt ttcacatgcg
      541 cgcccgcgct ggtcgcatca aatacaatgt cgatatcagc aaattcaggc atgttcatca
      601 gcccgatcac cccttcatgg gtggtggcga cgcccatacg tctggcacgc gccagaccgt
      661 cggacttagg atcaatgccg accatcaccg ccatctccag atgctgaccg tgacgcaaaa
      721 ttttaatcat cagatcggtg ccaatgttgc cagaaccgat aatggcgact ttacgcttac
      781 tcatgacaga cttccttttg gggctgcgct tgaaaatgtc gcagcaactg aacctatgcc
      841 ttcaatatgg gcttcaaaac gatcgcccgc attcaccgcc accatcgggc ctaatgcccc
      901 ggtaagaatg atatctccgg cgcgcagcgg ttcacccaga ctggccattt tgcgtgccag
      961 ccagacggcg gcattaagcg gatgtcccag gcattcgctg ccgcgcccgc tggaaacctc
     1021 ttcgttatta cgcgtcatct tcatggcgca gtttttcagg tctaaccccg ccggacgttg
     1081 cgccggaccg ccgatgacat acaccccgca ggaagcgtta tctgccacgg tatcgacaaa
     1141 ctgaatcgac cagtcgcgaa tgcggctccc caccacttcc agcgccggaa gtacccattc
     1201 aatggcgtta tacagttcgt cgaaggtgat atcagttgcg ggtaaatcgc ggttcaacac
     1261 cagtgcgatc tccgcttcaa tgcggggttg cagaacacgg gaaaaaggaa tgatttcgtt
     1321 atcgccataa cacatgtcgg caaataacgt cccaaaatcc ggttgatcaa cgcccagttg
     1381 ttgttgcact ttcggatgcg tcaggcccac tttacgccct accacgcgac gcccctgcac
     1441 aacgtcatat tgcacattta tgtgctgaat ggcgtaggcc gcttcagcgt tatcgatacc
     1501 aatcagatcg cgcagcggcg caatcgcttc gccctgctct gcggcgcggc gtaaatccgc
     1561 cgccagttgc tcaagagtat gcttcgtcat gaccattcct taagggcgtg cgaggaaatt
     1621 cagcaccaat tgattgaaag cgtcggcatg ttcccactgc gcccagtgac cacagtcgcg
     1681 gaagatatgc agttcagaac cggcaatgcc ggacagcaga cgcagacccg catccatcgg
     1741 cacaaagcgg tcgttgcgcc cccagacaat cagggtttgc gctttgattt ccgccagacg
     1801 tgggccaaaa tccgggaact gtttcggatt agcttccagg ctcttaacga agttttccag
     1861 gtgatcgcgg cgcgacagca tattattcag gcgcgcttca aacagggcgt cggtcaaatc
     1921 gctggtatca aaaacgaaga tatccatcat cagcttcagg ttttcgatag tcggctgacg
     1981 ataaagctga ttcagtcgct taataccttc ggttggcatc ggcgtaaaca aactcatgcc
     2041 gcccgtaccg ccgcccatca gcaccagttt gccgacgcgc tccggccatt taagggtgaa
     2101 cgccacagaa ctatggcccc ccatcgagtt gcccagcagg tggattttgg cgatatccag
     2161 ttgatccacc acgcttttca ggattcgtgc attaagatcc gatcgtgaac cactattaac
     2221 gatcgaatcg cttttgcccc aacccggaca atccagcagg attacccgat agcccgcctc
     2281 taccagcgga tcgatattgc ggctgaagtt cgcccagcca gtagcacccg ggccggaacc
     2341 atgcagcagg acaacggttt cgtcgccttg tccgcagtca ttaaaatgga tgcgcagcgt
     2401 tttacccgct tcttctacat tcagaaaacg gctggtggcg gcttcggttt gtggctgata
     2461 actcatcatc ttctcctgca tattcagttc tctgttctgg cgcttaacga gccaaatccg
     2521 gcaatccact ccgggattgg gcggtaataa cgcccttcgc tacgccagtt gccaaatgta
     2581 gaaatagcgg caaaagcggc gacccaggtt ttgatttcat gtgtcgactt tccggcaatg
     2641 gcggaaagct cttcgttact gacggcatcc agttcctgta tgcgtccctg ctccagcaaa
     2701 gtcatgaact ggttatccca aatcgggttg agcggatgca gagttctctg atcctcaaca
     2761 aacttctcag cggcgctaat cacccgttgc tgacgcaatt cgcgctcact ggcgggtaaa
     2821 tctttcccgc tccccaacag acggtcgcgc atatgggcat cggctttcgc cagttcgggc
     2881 accggcggct gatgggaaag cccaccggaa cccaggaaca gcacgcgttt attgagagtg
     2941 ctggtgaaac gtccaatggc ttcacccaac atgcgggtac gctggaaacc gggcagcggc
     3001 gtggcgacac cgttgatgaa cacaggcaga actggcacct tatccagccc accgagcagg
     3061 aactccagcg gctgggcgaa cccgtggtcc acctgcatac agtaagaaac ggcaagatcg
     3121 atcccgctct tcatgacagc atgcgcacag gcctccgcca gctccacagg cacgggcagc
     3181 tctcctgccg cactgccgaa atcaccaatt gccgtcgctc caacgcctaa acagaacggt
     3241 ggcatcacgt catagaaaaa gccgttgtag tgatctggcg caaacagcac caccagttca
     3301 ggggagaaag ccgcaatacg ctcgcgggcg ctggcaatca cgccattgac ctcatcgagt
     3361 acctcttgcg ccgggtcgac ataccccacc aacggcgagt gggaaagaca gtgaagataa
     3421 gcgtgcatat caggctacct tttcgacaga aacgtcggca tcagggcggg tcagcgtcat
     3481 caccgacgcc agtttattca gcgtattgcc cagggtttgc ggaatggcgg tggcggcaac
     3541 aaagcgatcc gggcgcatca ccaccagcga agcattgtgt tgcgcgaacc agctacgcag
     3601 acgaccttgc gtatcgccca cgcgtagcac gccgtcgtgg ttatcctgtg cggtatgaat
     3661 ttgcacttcc ggcaccacct gaatgaagcg tgtgcccaac gcgcgccact gctggatttg
     3721 ctcgtcgctc atcccccaca gtggattgca tccccagcca attaccgcga agttcgcgcc
     3781 aatcgcgtta tcgagcagcg tcacgtcgcc gttttccagc gtgactttcg gctgaataaa
     3841 catcttgccg accggagagt gcttcgcttc gccctcacgt accagcgcac cgccgtaata
     3901 ttgcggcatc ggcttgaagc gcatttcgag gaagtagcgt tttactggcg gcagataatt
     3961 cagtagccag gaaacgccgt cacgtaacgt accctgccag cgtttcggcg gagccagcac
     4021 gttgcccgtc gtcacggaca ggtcaatcat cgctttggcg tgatcgcgac gttcttgttg
     4081 ataggtatcg agcagcgcat cgcgggcttt cccctggata accaacgcca gtttccatgc
     4141 gaggttaaag gcgtcgcgca taccactgtt atagccctgc ccctgccata ccggcatgat
     4201 gtgcgcggca tcgcccgcca gcagtacgcg atcaatacgg aaacgttgcg ccagtcgcgc
     4261 gttgtgggtg tagacacgct ggcgaatcaa ttcaacattg tccggattag gcagcacttt
     4321 gcttaacagc ttgcgcatat tttgcggctc acgcagctgt tcttcggttt ctcccggcat
     4381 caccataaat tcaaagcgac gtaccgcatg aggcagcgcg gcagaaacat acgggcgcac
     4441 cggatcgcaa cataaataga tatgcggcgt acttaacgga tcgttggcga tatccaccac
     4501 aatccactga tttggcgcag ttttaccttc aaacggcaca ttcagggtgc gacgaacaaa
     4561 acttgcccca ccatcacagg ccaccagcca ctgggctttg actatttccc gctgcccttc
     4621 tgccgttttc aggtgcaagg tcacttcgtc attttgctgg ctgaaggcct ccagctcgcg
     4681 ggaaaacaag cagcgcacat taggaaaacg cgacacccct tccagcatca ccgcatcgac
     4741 ctgcggctga ataaaggcgt tacggcgcgg ccagccaaat tcatcggtca ttggctgaat
     4801 atcagcaaaa cagcggcctt tcggggtgag aaaacgcatc gcgtgccacg gcgtagtgtg
     4861 cggcagaaca ttctcgacca ggccaaccga ctgcatggtg cgcagcgcct cgtcatcaat
     4921 accaatcgca cgcgggtagt cgatcaactt atcgagtttc tccaccacca gcacgtcaat
     4981 gcccatctga ccgagatagt tcgccatcat cagcccaacc ggaccggcac cagcgatcgc
     5041 cacctgaacg ctatggttaa cagcaggctg gatgtcaggg tgttgtattg ccatttcagt
     5101 acctcacgac tcggacaaaa tgccgttgcg cgcacagtac ggcgcaactt attttgttaa
     5161 aaacatgtaa atgatttttt attgtgcgct cagtatagga agggtgtttt cggctacaat
     5221 caaaacatgc ccgaatgtgc accaggtgca caacgttgtt ttaactatag aaatgtcaat
     5281 taatatgcag aacaatgagc agacggaata caaaaccgtg cgcggcttaa cccgcggtct
     5341 aatgttatta aatatgttaa ataaacttga tggcggtgcc agcgtcgggc tgctggcgga
     5401 actcagcggc ctgcatcgca ccactgtgcg gcgactgctg gagacgctgc aggaagaggg
     5461 atatgtccgc cgtagcccct ccgatgacag ttttcgtctg accatcaaag tgcggcaatt
     5521 aagcgaagga tttcgtgacg aacagtggat ttctgcactg gcggccccgc tgctgggcga
     5581 tctgttgcgc gaagtggtat ggccaacaga tgtgtccacc ctggatgttg atgcaatggt
     5641 ggtacgcgaa accacccacc gtttcagccg cttatccttt caccgggcaa tggtcgggcg
     5701 acgtttaccg ctcctgaaaa ccgcctcggg cctgacctgg ctggcctttt gcccggaaca
     5761 agaacgcaag gaattaatcg aaatgttagc cgcccgcccc ggtgatgact atcaactggc
     5821 acgagaaccg ttaaagctgc aagccattct ggcgcgcgcg cgcaaagagg gttacggaca
     5881 gaactaccgc ggctgggatc aggaggagaa gattgcctct atcgccgtac cgctgcgcag
     5941 tgaacaacgg gtgattggct gtctgaatct ggtgtatatg gcgagcgcaa tgaccattga
     6001 acaggcagcg gaaaagcatc ttccggcgct acaacgggta gcaaaacaga tcgaagaagg
     6061 ggttgaatcg caggctattc tggtggccgg acggcgaagc ggcgtgcatt tacgttgaca
     6121 ccatcgaatg gcgcaaaacc tttcgtggta tggcatgata gcgcccggaa gagagtcaat
     6181 tcagggtagt gaatgtgaaa ccagtaacgc tatacgatgt cgcagagtat gccggtgtct
     6241 cttatcagac cgtttctcgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcgag
     6301 aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac
     6361 tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc
     6421 cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg
     6481 tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg
     6541 cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg
     6601 tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca
     6661 tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg
     6721 cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc
     6781 tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac
     6841 gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg
     6901 gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg
     6961 ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata
     7021 ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc
     7081 tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca
     7141 atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa
     7201 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     7261 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     7321 caggctttac actttatgct tccggctcgt atgttgtgtg aaattgtgag cggataacaa
     7381 tttcacacag gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg
     7441 tcgtgactgg gaaaaccctg gcgttacaca acttaatcgc cttgcggcac atcccccttt
     7501 cgccagctgg cgtaatagcg aagaagcccg caccgatcgc ccttcccaac agttgcgcag
     7561 cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg
     7621 gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca
     7681 cggttacgat gcgcccatct acaccaacgt gacctatccc attacggtca atccgccgtt
     7741 tgttcccacg gagaatccga cgggttgtta ctcgctcaca tttaatgttg atgaaagctg
     7801 gctacaggaa ggccagacgc gaattatttt tgatggcgtt aactcggcgt ttcatctgtg
     7861 gtgcaacggt cgctgggtcg gttacggtca ggacagtcgt ttgccgtctg aatttgacct
     7921 gagcgcattt ttacgcgtcg gagaaaaccg cctcgcggtg atggtgctgc gctggagtga
     7981 cggcagttat ctggaagatc aggatatgtg gcggatgagc ggcattttcc gtgacgtctc
     8041 gttgctgcat aaaccgacta cacaaatcag cgatttccat gttgccactc gctttaatga
     8101 tgatttcagc cgcgctgtac tggaggctga agttcagatg tgcggcgagt tgcgtgacta
     8161 cctacgggta acagtttctt tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc
     8221 tttcggcggt gaaattatcg atgagcgtgg tagttatgcc gatcgcgtca cactacgtct
     8281 gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg aatctctatc gtgcggtggt
     8341 tgaactgcac accgccgacg gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg
     8401 cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc aagccgttgc tgattcgagg
     8461 cgttaaccgt cacgagcatc atcctctgca tggtcaggtc atggatgagc agacgatggt
     8521 gcaggatatc ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc
     8581 gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc
     8641 caatattgaa acccacggca tggtgccaat gaatcgtctg accgatgatc cgcgctggct
     8701 accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt
     8761 gatcatctgg tcgctgggga atgaatcagg ccacggcgct aatcacgacg cgctgtatcg
     8821 ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg gagccgacac
     8881 cacggccacc gatattattt gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc
     8941 ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct
     9001 gatcctttgc gaatacgccc acgcgatggg taacagtctt ggcggtttcg ctaaatactg
     9061 gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc gtctgggact gggtggatca
     9121 gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg
     9181 cgatacgccg aacgatcgcc agttctgtat gaacggtctg gtctttgccg accgcacgcc
     9241 gcatccagcg ctgacggaag caaaacacca gcagcagttt ttccagttcc gtttatccgg
     9301 gcaaaccatc gaagtgacca gcgaatacct gttccgtcat agcgataacg agctcctgca
     9361 ctggatggtg gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc
     9421 tccacaaggt aaacagttga ttgaactgcc tgaactaccg cagccggaga gcgccgggca
     9481 actctggctc acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag aagccgggca
     9541 catcagcgcc tggcagcagt ggcgtctggc ggaaaacctc agtgtgacgc tcccctccgc
     9601 gtcccatatc atcccgcaac taaccaccag cgaaacggat ttttgcatcg agctgggtaa
     9661 taagcgttgg caatttaacc gtcagtcagg ccttctttca cagatgtgga ttggcgatga
     9721 aaaacaactg ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg ataacgacat
     9781 tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct ggaaggcggc
     9841 gggccattac caggccgaag cagcgttgtt gcagtgctcg gcagatacac ttgccgacgc
     9901 ggtgctgatt acgaccgctc acgcgtggca gcatcaggga aaaaccttat ttatcagccg
     9961 gaaaacctac cggattgatg gtagtggtca aatggcgatt accgttgatg ttgaagtagc
    10021 gagcgataca ccgcatccgg cacggattgg cctgacctgc cagctggcgc aggtcgcaga
    10081 gcgggtaaac tggctcggat tagggccgca agaaaactat cccgaccgcc ttactgcggc
    10141 ctgttttgac cgctgggatc tgccattgtc agacatgtat accccgtacg tcttcccgag
    10201 cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat ggcccacacc agtggcgcgg
    10261 cgacttccag ttcaacatca gccgctacag tcaacagcaa ctgatggaaa ccagccatcg
    10321 ccatctgctg cacgcggaag aaggcacatg gctgaatatc gacggtttcc atatggggat
    10381 tggtggcgac gactcctgga gcccgtcagt gtcggcggaa ttccagctga gcgccggtcg
    10441 ctaccattac cagttggtct ggtgtcaaaa ataataataa ccgggcaggc catgtctgcc
    10501 cgtatttcgc gtaaggaaat ccattatgta ctatttaaaa aacacaaact tttggatgtt
    10561 cggtttattc tttttctttt acttttttat catgggagcc tacttcccgt ttttcccgat
    10621 ttggctacat gacatcaacc atatcagcaa aagtgatacg ggtattattt ttgccgctat
    10681 ttctctgttc tcgctattat tccaaccgct gtttggtctg ctttctgaca aactcgggct
    10741 gcgcaaatac ctgctgtgga ttattaccgg catgttagtg atgtttgcgc cgttctttat
    10801 ttttatcttc gggccactgt tacaatacaa cattttagta ggatcgattg ttggtggtat
    10861 ttatctaggc ttttgtttta acgccggtgc gccagcagta gaggcattta ttgagaaagt
    10921 cagccgtcgc agtaatttcg aatttggtcg cgcgcggatg tttggctgtg ttggctgggc
    10981 gctgtgtgcc tcgattgtcg gcatcatgtt caccgtcaat aatcagtttg ttttctggct
    11041 gggctctggc tgtgcactca tcctcgccgt tttactcttt ttcgccaaaa cggatgcgcc
    11101 ctcttctgcc acggttgcca atgcggtagg tgccaaccat tcggcattta gccttaagct
    11161 ggcgctggaa ctgttcagac agccaaaact gtggtttttg tcactgtatg ttattggcgt
    11221 ttcctgcacc tacgatgttt ttgaccaaca gtttgctaat ttctttactt cgttctttgc
    11281 taccggtgaa cagggtacgc gggtatttgg ctacgtaacg acaatgggcg aattacttaa
    11341 cgcctcgatt atgttctttg cgccactgat cattaatcgc atcggtggga aaaatgccct
    11401 gctgctggct ggcactatta tgtctgtacg tattattggc tcatcgttcg ccacctcagc
    11461 gctggaagta gttattctga aaacgctgca tatgtttgaa gtaccgttcc tgctggtggg
    11521 ctgctttaaa tatattacca gccagtttga agtgcgtttt tcagcgacga tttatctggt
    11581 ctgtttctgc ttctttaagc aactggcgat gatttttatg tctgtactgg cgggcaatat
    11641 gtatgaaagc atcggtttcc agggcgctta tctggtgctg ggtctggtgg cgctgggctt
    11701 caccttaatt tccgtgttca cgcttagcgg ccccggcccg ctttctctac tgcgtcgtca
    11761 ggtgaatgaa gtcgcttaag caatcaatgt cggatgcggc gcgagcgcct tatccgacca
    11821 acatatcagg acggaatgat cgcaatgaac atgtcgatga cagaaagaat aaaagcaggc
    11881 aagctattta ccgatatgtg cgaaggctta cctgaaaaaa gacttcgtgg gaaaacgtta
    11941 atgtatgagt ttaatcactc gcatccatca gaagttgaaa aaagggaaag cctgcttaaa
    12001 gaaatgtttg ccacggtagg ggaaaacgcc tgggtagaac cacctgtcta tttctcttac
    12061 ggttccaaca tccatatagg ccgcaatttt tatgcaaatt tcaatttaac cattgtcgat
    12121 gactacacgg taacaatcgg tgataacgta ctgattgcac ccaacgttac tctttccgtt
    12181 acgggacacc ctgtacacca tgaattgaga aaaaacggcg agatgtactc ttttccgata
    12241 acgattggca ataacgtctg gatcggaagt catgtggtta ttaatccagg cgtcaccatc
    12301 ggggataatt ctgttattgg cgcgggtagt atcgtcacaa aagacattcc accaaacgtc
    12361 gtggcggctg gcgttccttg tcgggttatt cgcgaaataa acgaccggga taagcaatat
    12421 tatttcaaag attataaagt tgaatcgtca gtttaaatta taaaaattgc ctgatacgct
    12481 gcgcttatca ggcctacaag ttcagcgatc tacattagcc gtatccggca tgaacaaagc
    12541 gcaggaacaa gcgtcgcatc atgcctcttt gacccacagc tgcggaaaac gtgctggtgc
    12601 aaaacgcagg gttataatca tcagcccaac gacgcacagc gcatgaaatg cccagtccat
    12661 caggtaattg ccgctgatac tacgcagcac gccagaaaac cacggggcaa gcccggcgat
    12721 gataaaaccg attccctgca taaacgccac cagcttgcca gcaatagccg gttgcacaga
    12781 gtgatcgagc gccagcagca aacagagcgg aaacgcgccg cccagaccta acccacacac
    12841 catcgcccac aagaccggca attgcagcgg caaccagata aagccgcaga accccaccag
    12901 ttgtaacacc agcgccagca ttaacagttt gcgccgatcc tgatggcgag ccatagcagg
    12961 catcagcaaa gctcctgcgg cttgcccaag cgtcatcaat gccagcaagg aaccgctgta
    13021 ctgcgcgctg gcaccaatct caatatagaa ggcgggtaac caggcaatca ggctggcgta
    13081 accaccgtta atcagaccaa agtaaacacc cagcgtccac gcgcgaggag tgaaaaccac
    13141 gcgaaccgga gtggttgttg tcttgtggga agaggcgacc tcgcgggtgc tttgccacca
    13201 ccaggcaaag agcgcaacaa cggcaggcag cgcccaccag gcgagtgttt gataccaggt
    13261 ttcgctatgt tgaactaacc agggtgtcag ggcggcacca agcccaccgc cgcccatcag
    13321 agccgcggac catagcccca tcaccagtgg cgtgcgctgc tgaaaccgcc gtttaatcac
    13381 cgaaggcatc accgcctgga tgatgccgat ccccacccca ccgagcagtg cgctgctaag
    13441 cagcagcgca ctttgggggt aaagctcacg catcaatgca ccgacggcaa tcagcaacaa
    13501 actgatggcg acgctgcgac gttcgctgac atgctgatga agccagcttc cggccagcgc
    13561 cagcccgccc atggtaacca ccggcagagc ggtcaacagg gcagccacgc taaagctcat
    13621 tccgctcgcc tggcgcaatt gcggtagcag tggcccgacg gaggtgagca gtggtcgcat
    13681 attaagacca atcagcacca gaaccagcag tatcgtgaac gtcttttgat ggttgcgcat
    13741 ggctgtcaga acggtttggt cggcagatat ttaccatcta aggtgatgac cgcacgttcg
    13801 ccaccttccg ggtccgccac tttcttaacg tcgagtttga agttaatcgc gctgataatg
    13861 ccatcgccaa acttctcatg aaccaacgct ttcagggttg tgccgtacac ctgcaacatt
    13921 tcatagaaac gatacatcgt tgggtcggtt ggaatacggt catcaatgca gccacgcagt
    13981 ggaatcatct gcaacagtag aatggcgtct tcgtcgagat ccagcttcgc tccgaccagg
    14041 cgggcggcgt cggcaggaag cgcctgctgc cccagcaaag ccgcggttac gaaggcttct
    14101 gccagaccgg taccgtcggc aatctcggca aatgagagat cttttttagc tttgctgagc
    14161 aaaatggcat cggcaagatc aagacgaata ttgcggttaa tttgcgactg aatcatggtg
    14221 catctcctga tggtttaaaa ataaggttac gctgcggtcg gttggcgtag cggtatggca
    14281 caaacgcgcg gattagctgc cagtggcaca aactggcggg ttgcgccgtc aaacgctgcg
    14341 atgctgccgc tttcaatgtc gtagacccag ccgtgcaggg cgatccgccc ctcttcgagc
    14401 gccaggcgca ccgatggatg agtttgcaaa ttagccaact gagcaatgac gttttcacgt
    14461 accatcgccg cagcttttga cggtaaatcg gaatgcgggc gcgcctcatt aacgacgcgg
    14521 gctgaatcgg cataacgcag ccagtgggag acggcaggca tatggtccat gcactgacag
    14581 ctggcaatgg cggtcatcgc gccacagttg gaatgaccgc aaatcacaat gtcagatacc
    14641 cgaagcgcag cgacggcata ctccaccgaa gcagaaacgc caccgggttc cggcccgtag
    14701 gaagggacga tattgcccgc gttgcgaata acgaacagat cgccaggctc acgttgcgtc
    14761 accagctcag ggaccagacg gctgtcggag caggagataa aaagtgtgcg cgggctttgc
    14821 tgtgtcgcca gctgtttaaa caaggcttcc cgcttcggaa atgcctcgcg ctggaattta
    14881 aggaatccat caataatctc tttcaccgtt aacctctgtc tgtctctgga atgagaggca
    14941 gactactcgc cgattgtcat aaggtaaaag tctcatttat gatgagttct attggattta
    15001 cttataggtt ggcgaatgct ctctcgacat atcaattatt ttcttgccgt ggctgaacat
    15061 ggcagcttca cccgtgccgc cagtgcgttg cacgtctctc agcctgcgct ttcccagcag
    15121 attcgccagt tagaggagag tttaggcgtg ccgctgtttg accgtagcgg gcgaacgatt
    15181 cgtctcaccg atgcaggaga agtctggcga cagtacgcca gccgggcgtt acaggaactg
    15241 ggagcgggta aacgggcgat tcatgatgtt gccgatctga cgcgaggatc gctgcgtatc
    15301 gccgtcaccc ccacctttac aagctacttt atcggccctt taatggcgga tttctatgcg
    15361 cgctaccccg gcatcacgct ccagctacag gaaatgtcgc aggagaaaat cgaagatctg
    15421 ctttgtcgcg acgagctgga cgttgggatt gccttcgcgc ctgtgcattc gccggagctg
    15481 gaggcaattc ctttactgac agaaagttta gcgttagtcg tggcgcaaca tcatccgctg
    15541 gctgcctgtg aacaggtggc gttgagtcgc ttgcatgatg aaaaactggt cctgctcagc
    15601 gcggaatttg ccaccagaga gcaaattgac cactactgcg agaaagcggg gctacatcca
    15661 caggtggtca ttgaggcgaa ctcaattagc gcggttctgg agctgattcg ccgcacttcc
    15721 ctttccacat tgttaccagc agcgattgcc acacaacatg acgggcttaa agcgatttct
    15781 cttgccccgc cactactgga gagaacggcg gttttgttgc ggcggaaaaa tagctggcag
    15841 acagccgccg cgaaggcatt tttgcacatg gcgttggagg aatgtgcgga tgttggcgag
    15901 aatgaatcac ggtagccact gacacaggtc agtggctctt caacacagag gaaattaccg
    15961 tttgtaatcg atggcttctg gctgctccag atatacggtg gtttgtgccg gttgtgtact
    16021 ggcaatcacc ttgccgccac gtaccgaata acgtaccgga acctgacggc gcagcgcatc
    16081 aaatccattt tcagccggca ggataatcag gtttgcgctg tttccggcgg caatgccgta
    16141 atcctgcaaa ttcaatgttc tggcactgtg gtgggtgatt aaattcaggc catcgttaat
    16201 ctgcccgtag cccatcaact ggcaaacatg cagccccata tgcagcactt gcagcatatt
    16261 cgccgttccc agcggatacc acggatcgaa gacatcatcg tgaccaaagc agacgttaat
    16321 gccggactcc agcatctctt taacgcgcgt gatgccgcga cgttttggat acgtatcgaa
    16381 acgtccttgc agatgaatat tgaccagcgg gttggcgaca aagttaatac cggacatttt
    16441 cagcaagcgg aacaggcgtg aggtatacgc cccgttatag gagtgcattg ccgtggtgtg
    16501 gctggcggtg actcgcgcgc ccatgccttc acggtgcgcc agggcagcaa cggtttcgac
    16561 aaagcgcgac tgctcgtcat cgatctcatc acagtgaacg tcgatgagac ggtcgtattt
    16621 ttgcgccagg gcgaaggttt tatgcagcga ctccacgccg tattcacggg taaattcaaa
    16681 atgcggaatc gcccccacta catctgcccc taagcgtaac gcctcttcca gcaacgcttc
    16741 accgttggga tacgacaaaa tcccttcctg agggaaggcg acaatttgca gatcaatcca
    16801 cggcgcgact tcctgcttca cttccagcat tgctttcagc gcagttagcg ttgcatccga
    16861 aacatcgaca tgggtacgca catgctgaat gccgttggca atctgccatt tcagcgtttg
    16921 ccaggcgcgt tgtttcacat cgtcatgggt taataacgct ttgcgctcgg cccagcgttc
    16981 aatgccttca aacagcgtgc cggactgatt ccagttcggt tgtccggcgg tttgcgtggt
    17041 gtccaggtga atatgcggct ccacaaacgg cggtataact aaaccttgtt cggcatccag
    17101 gctgttttca gttatgggca tcacgccgga ttgcgcatca atggcgctga tttttccgtc
    17161 ctgcagatga atctgccaca gcccctcttt gcctggtaac cgggcgttaa taattgtttg
    17221 taaagcgtta ttcgacactg ttaacctcca catgcgtcat tgctgctgtc gttttacgat
    17281 tcaaaatcgg gttaaggatc agatagctca gcgcgccacc taataccgcg ttgaccggaa
    17341 caattcccgg taaccagtgg cctgcggcaa tccccagcgc gaccgccaga atcgccaccc
    17401 aattgacact catcatacgc gtggtcgcaa agtgctcata gcggcgacgg ttcatcagat
    17461 agtcggcgat gatcacgccc cctaccggag gaatagctgc cgaaaggaag gtcaaccagc
    17521 cgacaaaatt gttatacagc cataatgcgc agaccgtacc gataataccg ttgattaccg
    17581 aaagggtttt gctcgacata ccggtaatgt tggcgaaacc taaacccgac gcatagagtg
    17641 cgttatcgtt ggtggtccag atattcagcc ccagcaccac aatcgcgggc agcagcagtc
    17701 cctgagcaat catcacatca gagatatctg ccatccccag tgccgcagcg cccgctgcac
    17761 cgaaaataaa catcaacgag ttgccgagga aaaaggccac catcgccacc agcaccgcca
    17821 gtttggcatt gcgaccaaac cggacaaagt cagcggtgag cgtacccgca ctgataaatg
    17881 accccacaac cagcgccagc gcgacattga aatctaacgg ttgtgcggga acgaccgctt
    17941 ttaaggcgtc caggccgccc atgccgttaa cggccagcca cacggaataa ccgccaaggc
    18001 aggcaatagc cggaaccgca atcaccgaaa gaactgtcag cgccgaaatg ccaaagaaga
    18061 cggtgacggt catcagtaaa ccggaaacgg caatcagcaa attaatatcc agcccggttg
    18121 ccttgcccac cggaatagca aacatcgcca cgccaacgcc aaaccagcca acctgagttc
    18181 cgcccagtag cagtgaaggc agccatgagc ctttaacacc aaacgagaag cgagcaagaa
    18241 gatgagtggt caggccggtt tttgcgccaa tgtaaccgag aaatgaagtg taaataccga
    18301 ggagaagatt accgatgaga actgcgagga agaaatcatg atagctaaga ccggttccga
    18361 gagtgccgcc ggtccacata ctggcggaaa agaaggttaa tcccagcatg acgaacgtca
    18421 atgccaatac ccctttccgc gccgactgcg ggactggccc ctggctaaag ttgttatctt
    18481 gcgacacgaa attccccctt tttgctgttt gaaacccaaa aaatccgccg cattctattc
    18541 atctgaagat aaaaagcaat cgttttcgtg gggaaatata ttttttatat gaatgagtgc
    18601 aaaaaggtgg gggaaaaatt atcggtgtaa aaacagtggg taggcataat aagacgcggt
    18661 aagcgtcgca tcaggcatcg gcgcggggtg tcggatgcga cgcgagcgcc ttatccgacc
    18721 tacggttcag gcctgtaggc ataataagac gcggtaagcg tcgcatcagg catcggcgca
    18781 tggtgtcgga tgcgccacgc gacgcatccg acaatacctt actcttccat cgcctggcgg
    18841 atctgatcca atgacgcagg atcatcaatg gtcgtcagat ccccaggatc gcgttttcgg
    18901 caattg
//
LOCUS       DAMZET010000081        18871 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.081, whole genome shotgun sequence.
ACCESSION   DAMZET010000081 DAMZET010000000
VERSION     DAMZET010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.081"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..803)
                     /locus_tag="POB34_002815"
     CDS             complement(<1..803)
                     /locus_tag="POB34_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="metallo-dependent hydrolase superfamily; deaminase
                     with metallo-dependent hydrolase domain; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HDI5919725.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALD"
     gene            complement(813..1763)
                     /locus_tag="POB34_002816"
     CDS             complement(813..1763)
                     /locus_tag="POB34_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HDI5919726.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(1906..3324)
                     /locus_tag="POB34_002817"
     CDS             complement(1906..3324)
                     /locus_tag="POB34_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDI5919727.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEATELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(3324..4871)
                     /gene="fdrA"
                     /locus_tag="POB34_002818"
     CDS             complement(3324..4871)
                     /gene="fdrA"
                     /locus_tag="POB34_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDI5919728.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(4861..5724)
                     /locus_tag="POB34_002819"
     CDS             complement(4861..5724)
                     /locus_tag="POB34_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDI5919729.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFNGLFRHGDKVQFDEQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALVLFIPGHPAEKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHSFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(5764..6369)
                     /locus_tag="POB34_002820"
     CDS             complement(5764..6369)
                     /locus_tag="POB34_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDI5919730.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDLTCLNPFLMSCLNGDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            6627..7124
                     /locus_tag="POB34_002821"
     CDS             6627..7124
                     /locus_tag="POB34_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDI5919731.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGISGFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            7216..8148
                     /locus_tag="POB34_002822"
     CDS             7216..8148
                     /locus_tag="POB34_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI5919732.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(8190..9278)
                     /gene="pdeL"
                     /locus_tag="POB34_002823"
     CDS             complement(8190..9278)
                     /gene="pdeL"
                     /locus_tag="POB34_002823"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HDI5919733.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGSNSHRYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFVSEWVMKAGG"
     gene            complement(9774..9848)
                     /locus_tag="POB34_002824"
     CDS             complement(9774..9848)
                     /locus_tag="POB34_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919734.1"
                     /translation="MCDILLNVLNIVFIGIAIILVIIC"
     gene            complement(10154..12187)
                     /gene="betT"
                     /locus_tag="POB34_002825"
     CDS             complement(10154..12187)
                     /gene="betT"
                     /locus_tag="POB34_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HDI5919735.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            12316..12903
                     /gene="betI"
                     /locus_tag="POB34_002826"
     CDS             12316..12903
                     /gene="betI"
                     /locus_tag="POB34_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HDI5919736.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVAGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            12917..14389
                     /gene="betB"
                     /locus_tag="POB34_002827"
     CDS             12917..14389
                     /gene="betB"
                     /locus_tag="POB34_002827"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HDI5919737.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSALQGQKIWASMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            14403..16073
                     /gene="betA"
                     /locus_tag="POB34_002828"
     CDS             14403..16073
                     /gene="betA"
                     /locus_tag="POB34_002828"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HDI5919738.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDMGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            16286..16954
                     /locus_tag="POB34_002829"
     CDS             16286..16954
                     /locus_tag="POB34_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919739.1"
                     /translation="MREQIKQDIDLIEILFYLKKKIRVILFIMAICMAMVLLFLYINK
                     DNIKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIR
                     LEWSGDKRALPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(17197..17892)
                     /locus_tag="POB34_002830"
     CDS             complement(17197..17892)
                     /locus_tag="POB34_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HDI5919740.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVILFSAAERGRSLSLLP
                     EYSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(17885..>18871)
                     /locus_tag="POB34_002831"
     CDS             complement(17885..>18871)
                     /locus_tag="POB34_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HDI5919741.1"
                     /translation="DPPSHVVVPAIHKDRHQIRRVLHERLGYEGPETPEAMTLFIRQK
                     IREDFLSAEIGITGCNFAVAETGSVCLVTNEGNARMCTTLPKTHIAVMGMERIAPTFA
                     EVDVLITMLARSAVGARLTGYNTWLTGPREAGHVDGPEEFHLVIVDNGRSEVLASEFR
                     DVLRCIRCGACMNTCPAYRHIGGHGYGSIYPGPIGAVISPLLGGYKDFKDLPYACSLC
                     TACDNVCPVRIPLSKLILRHRRVMAEKGITAKAEQRAIKMFAYANSHPGLWKVGMMAG
                     AHAASWFINGGKTPLKFGAISDWMEARDLPEADGESFRSWFKKHQAQEKKNG"
BASE COUNT         4583 a         5054 c         4722 g         4512 t
ORIGIN      
        1 tagtccagcg caatctgaaa catggtgtcg agggattttt ccatcgcgcc atcaacactg
       61 gtcgggtcca gcccgccgac gtaatgcgca cccgcctgca tcgcttcgcg cattaagggt
      121 tccgatttcg acagcagcaa accgtgctgc gggaaggcga cgatttcaca ctcgaagccc
      181 gcctgacgcc gcgccagcac cgcctgcaaa ttttgcagat tttttaggcc ggaaaccggt
      241 tcgatattgc agtggctgcg ggcaatggtg gtgcctttcg actgcaatag atcgatcagt
      301 ttttccgccc gctcctgcgt atacggttgt aactccggca gcattttctg ctcgagtttg
      361 atcatgtcct ggatagtggt gcctgccgga cgattgagcg agcgccacgg cccgccgtag
      421 aaggttttgt cgagatgaat atgcatgtcg cgagtggtgg gtagcatcaa cttaccgcca
      481 gcgtcatagt gcggcagcgt ggcgtccgga tgctgcttgt tctcgcgcag ggcgacaatt
      541 ttgccgtcct gaatctccac ggtctggcgc gccgtgcggg tctgcaccgc cacaccgttt
      601 tcgtagtcaa aaccggtttc cagcagcacg ttatcgagat agtaatgcgg gtcagtgatt
      661 ttcatggtgt tgttcgcttc gcagtttagg gtgccagcta ccgccgcatg ggcgagcggc
      721 acagaggtac caaacagcgc ggcggcggtg accatcttac cgctctggct cagaaattca
      781 cggcggctat tgctttcttt catcctacgt ccttacgttt taataatatg ggtgcccgtt
      841 tcaccgcgca gcgccgcagg caggcattcc ggcgtggtga taatcacttc tttgccgccc
      901 tgctccagga acgtcaggct ggcgatgatt tttggcaaca tgctgccggg cggaaaatgc
      961 ccttcctgca tatagcgagt catggtggca atatccaccc gatccagcgc ctgctgctgc
     1021 ggtttaccaa agtggataca gactttttcg acgccggtgg tgatcaccag aatgtcggcg
     1081 tgaatttcac gggccagcag cgcggtagag agatctttgt caataaccgc gtccacgctt
     1141 tggtaatccc ccgcttcagt acgcactacc ggaattccac cgccgcccgc gccaattacg
     1201 acaaaacctt gttggatcag cgccttaatg gcgggtgctt cgacaatacg tttcggttcc
     1261 ggcgaggcga ccacgcggcg atagccccgt ccggcatctt caacaaaaca ccagtcaggg
     1321 tttgcctttt gtagtttgtc acgctggctt tcactgaaga atgcaccaat gggcttggtg
     1381 ggatgggcaa aacctggatc gtttttatcc acttccacct gagttaccac ggtgacggct
     1441 tttttctcgc cgtgacgcgc cagccggtta ttcagcgcct gttggatcag atagccgatg
     1501 ccgccctgcg tatctgccac acagttcgcc agcggcgtta agggcagccc ttcgcgctcg
     1561 tgggcaatct ccgcacggcg taaatccagc ccgacctgcg gcccgttacc gtgggtcagc
     1621 acaatgtcgt aatcggaagc cagcatttcc agcaccgtat cggccacggc tttcaccgcc
     1681 tccgcctgat gctcaatcga ctggctggcg ttatctttga taatgctgtt gccaccaatg
     1741 gcaacgacca caagctcttt catgttgtta tgtccgctac agagggtgat taggttatga
     1801 ccaacgccag actgcctgat gcgctacgct tatcaggcct acaattctcg ggccatccgt
     1861 aggccggata aggcgttcac accgcatccg gcataaacat gtaagtcaga cgccgtaacg
     1921 ttcgcaccag ccgagaatgg ctttctcgaa gcaggcgaat ggtggatgca caatgcccgc
     1981 gccaatcatg ccgacgcccg catctttatg ggcgatggcg gtgttgatca ccggcagaat
     2041 gccgctgcta cccacgcggg tgatgtcgat tgccgacggc acgcccataa aaccgagcag
     2101 cggaatggtg acgttggggt tttcgccaag ggtgatttcg cgcatctgac gggagaaatc
     2161 gatagcctct tccaccgtgc cgcccaccag tgcgacgatt gcaggcgcgg tcgccatggc
     2221 aaacccgcca ataccgtagg tttctgtgat ggcgctgtcg ccgatatcca gccccgaatc
     2281 ttccggctta taaccagcaa acatcgggcc gatgacctgc tgcgctgggc cggtaaacca
     2341 ctgccccggc aggccgctga cccgcaggcc gaactcgacg ccgttacgcg ccatggtggt
     2401 gaccacggtg ctgtattcga tgccgtgcgc cgcatccatc gccgctttac acatcgccat
     2461 ccacgtcggg ccggagaagt agtcgctgct ggcgacaaac tcgaacactt cgcgctgttg
     2521 ctcgacggaa tagcctgcct gaatgatccc cggcgtcagt gcctgaatca gcagcgtcgt
     2581 cccggcgtta ttgcggttat ggcactcatc gcccatatgc agcgcctgcg ccagcattaa
     2641 gcgcagatcg atctcaccga taattttcat cgcgtcgcgc agcattggcc cctgcacatc
     2701 gcgcatccag ttgaggcggt cgatcacgct ctgatcgttg gcacccatac gcaaaatctt
     2761 cgccatctgc tcgctcatat tggtataagc gatgttgccg taggttttgt ttttcacgat
     2821 gtgcataaac atcgaggccg aggtaacgcc cgccatcgat cccacgcagt cgtgttcgtg
     2881 gcacggcgag aaggtgatct ccccggaggc cgccagttca gtcgcctcat cgagatcttt
     2941 cgccagccct tcgaacacca acgccccggt gaccgcgcct ttcatcgcgc cgcacatttt
     3001 ttcccaggtg atcggcggcc cggcgtgaag aatggttttc ggcgtcatgc ccggcaccac
     3061 gttaatcgcc tgatcaaaac caatcagcac cggatgcgac tggataatgc gctccagcgc
     3121 ctgctggtta gcggcggcga ttttgtctgc cagcggcgaa tcggcaatgt tatccagcgc
     3181 ctgcaccacc tgcatattgc cctgccccgg cggcgtccag tcgagttgag tcacttctac
     3241 atgctgcttt ttcaggtcat cgctaaacat ggcgataccg acgttaataa cgttcaacgg
     3301 ttggctaaac agtgactggc tcatcaggct tcctcccctt tgcagataaa ttcacgcgcc
     3361 agcaatccgg tattggtgct gctgctcgcc agaatcactc cggcatcaag cagcatctgg
     3421 ctttgttgtt ctaatgatgg cgtatcgaga tcggtgccca gcacataggc gagaatgatc
     3481 aactcgcgtc cttcggcggc ggcgatcgct ttcgcttctt tgatcgcctc gatggtggag
     3541 ccgaccggat cttcatgcga tccaaatccg agcacaaaat ccatcacgat caccgccact
     3601 tctggatcgc gcgcttcttc gatcaagcga ctgatgcggt tggtggggtc aatcattgga
     3661 tgcggcttgc cgttggtgaa gtcgtcatcg ccaaagtcga ggaaggtgtg tttgatgctg
     3721 cggttgatat cttgcaggcg gaattcagga tcgggctgaa tgttgctgta gacatcgcca
     3781 tgtttttcca tcaccgcgaa catggtttcg tcgcacagcg tgccgccgca gaacaggcca
     3841 cgtatgtatt tctgctgcgg ttgcagacgc gcacgcacat cagcaatcaa cggctggtta
     3901 agcgtatgca ggtcgagatt ttcctgtttc acgccggaga gcatcaccgc ttttagcgcc
     3961 gcctctttgc tgccgcgggc aaactgtagc ccctgctcat ccaccggcgt ttcaacgcga
     4021 ccgaggaagc agaccaccac cggcttgcgg caggcgcgcg cgcgttccag cactttacgg
     4081 gccaccgcag gcgcgggcgg tttggagata agcacaatga tttcagtttg cggatcgttt
     4141 tccagcatcc cgatggcgtc gagcatcatc aggccgccga ttttctcgct caggtcgcgc
     4201 ccgccggtgc caatcagttg tgaaacgccg ccaccaaatt catgaatgcg gacgctcagc
     4261 tcctggctgc cggtgccgga tgcgccaaca ataccgatgt taccgcgacg cacggcgtta
     4321 ccaaagcaga gcgccgcgcc gttgataatc gccgtgccgc agtccggccc catcatcagc
     4381 agcccttttt catgggccaa ttgtttgagc gccagttcat cttcaaccga gacgttatcg
     4441 gaaaacagca tcacgttgag atcgttttgc agcgcctgac gcgcttcgcg agcggcaaac
     4501 agaccgttga ccgaaatcac cgccaggtta ctttccggga tatgcttttt ggcgctggca
     4561 atagtggcgt aacgcgcctc gtgcgagccg ctttgcgctt tggtgttgaa cagttcttca
     4621 atctccacca gcaactgctc gttgtccgca cccgatttac cattgatgac aatcatcagg
     4681 tcgccgtttt tcgcctgttc cagctccggc gtcagcagtc ctaaattttt cagcacacct
     4741 ttgttcattt cggtcgccat cgccacaaat gcctgctcga cgccgtcgag tttatttgca
     4801 cgcgtggaga tagacatcag cgagacagaa tcaaaatagg tattcggttt aatgactatt
     4861 ttaactgaca taattccctc cgtaggtttg gctcagcgca caaccatcgg ccatgccata
     4921 cagcatgtcg cagccggaac tggagccaat atgtttaatt ttttctattg cctgagttgc
     4981 gttcccaggg atgtcataaa taatgttgtg aataaaacta tgaatatttt cccggcaacg
     5041 ttgttgtaat gcggcttcca gcgttatagc acttaataat gtggtattat ttctgccgcg
     5101 ttgcagaccg agataaaatt cctctttgta tttttccgcc ggatgcccgg gaataaataa
     5161 aacaagcgcc agacctgtta aataatcgtc ggctgatggc gtaaggccaa tccctaaccc
     5221 catcatactg gcgaccgctg aggcgatatt ttgtttatcg tttacggcct gaataactgc
     5281 ccgtcgttta atatgtaatt ctttatttaa cgcctgataa aacggattat cgccgcgata
     5341 attaaacagg gtttcatttt cccccaggtg ctgatgaata atgtcgtgcc actgttgcca
     5401 ggcgataagg tgaaaattca cagcggtcat ttgcaaggtt ggggacagcc aacgccgaca
     5461 acgactcatc tctatatgaa gatgttgacc aaccgtaatg ccttgctcgt caaactgaac
     5521 cttatcacca tgccggaaca gaccattaaa gtgagtgagt gccaaccgac aactgtttgg
     5581 cgcattgtcg tactcttcac aaagcagcgt cagcaactgc tggcgagccg ggagtgcgat
     5641 atttaccgct ctggcaaaga cctgttcaac ctgtccttgc ccccgctgcg ccagaaaatc
     5701 cgcatccgca gttagcgccc acacacaccg tctttctggt gtgttgatag caagcctccc
     5761 ggtttatgca cctgcggcaa tcagtaactg cgcaatctct tcaaagcccc gttcccgcgc
     5821 cagttccagt ggcgttttgc catatttatc ggtcagatgc gggctggcac cgtgttccag
     5881 caataactgg acaatcgcct gctgtttaat cccgccatca ttaagcacaa tcgcttccag
     5941 cagcggtgtc cagccaacat ggttggtctg gttaacgtta atttccgtat gcgccaaaag
     6001 ctcttttaca atacttaaat ggcctttttc acaggcaggc gtcaggccga caccgccaaa
     6061 acgggtgacg caattaaggt ctggtttagc cggtaaaata attcgtagca gcgttaaatc
     6121 accgttcaga cagctcatta aaaaaggatt taaacatgtc aggtcttgtt tattaatatc
     6181 cgctccggca tcaattaatg cctgaacgca agcatattgc tgatataaac ttgccagcgt
     6241 aattgccgtt ttcccctggc gatcgcaggt attaatatcg acacccagcg caagacaggt
     6301 ttttacttta tcgatatcgc cctgttgtgc agccaataaa tagtcggcag gtagattttt
     6361 tatcgtcatt cacattcccc tggtttttgt ggctgttttc gatgaattga gaatagcaac
     6421 gataatctga taaaagaacc cgcttaaatt taattcgccg tatttcgcct ttttgttgaa
     6481 agatgttcaa caatcttttc gcagtgtgcg gtagtgcaat atttgtttct ttgacccgat
     6541 accctttcac tcaatcaggt tttatttctc tatagtctgc acatgtaaga gtagctttcg
     6601 tccatgtcta accagagggg aacaagatga acggactcac ggcaacgggt gtcaccgtcg
     6661 gtatttgtgc agggctttgg cagttagtgt cttcacatgt cgggctctct cagggatggg
     6721 aattattagg aaccattggc ttcgtcgcct tttgcagttt ttatgccgca ggcggcggta
     6781 aatcggggtt tatcagaagt ctggcggtta attattccgg gatggtgtgg gcatttttcg
     6841 ccgcgctggc tgccggttgg ctggcctcgg tgagtgggat ttctgggttc tgggcgagcg
     6901 tgattactac cgtgcctttc tcagcggtag tggtctggca agggcgtttc tggttactgt
     6961 catttattcc cggtggcttt ctcggcatga cgctgttttt tgccagcggg atgaactgga
     7021 cggtgacgtt actgggtttt ctggcgggta actgcgtggg gataatttca gaatatggtg
     7081 gacaaaaatt aagcgaagcg acgactaaac gcgacgggta ttaaaagtta ttcaaaaatg
     7141 attatctatc tttaaccgat acctctacag tcatagctgg agaaattcat agtagataat
     7201 ggcgagaatg gtttaatgaa ctcaattttt accgaagaaa acctgttggc atttactacc
     7261 gctgcgcgtt ttggcagctt cagtaaagcc gcagaagagt tgggtttaac cacttccgcc
     7321 attagctaca ccattaagcg tatggagacg gggctggatg tggtgctgtt cactcgcagt
     7381 acccgcagca ttgagttaac ggagtccggg cgttattttt tccgcaaagc taccgacctg
     7441 ctgaatgatt tttatgccat caaacgtagc atagatacta tttcgcaggg catagaagcg
     7501 cgtgtgcgca tttgtattaa tcagctgctt tatacgccca aacacaccgc gcgcttatta
     7561 caggtgctga aaaagcagtt tcctacctgc cagattaccg tgacgaccga agtgtataac
     7621 ggcgtctggg atgcgattat taataatcag gccaatatcg ccatcggcgc acccgataca
     7681 ctactggacg gcggcggtat tgattacact gaaattggcg cgattcgctg ggcttttgct
     7741 atcgccccgg atcacccgtt agcctttgtg ccggaaccca tcgccgaaag ccaactgcgt
     7801 ctctacccta atattatggt ggaggacacc gcacatacga ttaataaaaa agtgggctgg
     7861 ttgctgcatg ggcaggagtc aattctggtg ccagatttca acaccaaatg tcagtgccag
     7921 attttaggtg aaggaattgg ttttttgccg gattacatgg tccgtgaggc gatggcgcaa
     7981 tccttgctgg ttacccgaca aatccataac ccgcgccagg attcacgcat gttactcgca
     8041 acccagcact ctgccaccgg tcaggtgacg cagtggatta aaaagcaatt tgcgccaaat
     8101 ggcatattaa cggggatcta tcaggattta ctgcatcggg agaattaatc gctgccaacc
     8161 tcccgatgcg ctgtgaatag cggtttacat caaccaccag ctttcattac ccattcagaa
     8221 acaaatttat tacccggtac tggcggggag tacaaatagc cctgcaaaaa gtgaacgcct
     8281 ttaccgatca ttaaatccgc ctgctcctgg gtttctaccc cttccgctac gatactcaga
     8341 ccaggcttac gcgccagttc gacaatattg tccacaatat gaccggagat ttcgtcgaca
     8401 ctcgccattt gcacaaatga cttatcgatc ttaataaaat cgaccgggaa cgcctgcaag
     8461 taacgatagg tcgcataacc cgtaccaaag tcatccagcg caaaggtaat gttgtgctga
     8521 tgaaggctgt caaatatcgc tctggcttct ggcgttaccg gaatagggtt acgttccgtt
     8581 agctcgagaa ccagtttgat tttatcgttt cctaatttct tgaccaggtt caggcattct
     8641 ttttcaaatc ccgcggccaa aaaacaaccc gccgagacgt tgataccaat atggaaatta
     8701 tccggcagca aatgttttac cggcatcaga atatccgcag tctgtttcat cagttggcgg
     8761 gtcattatga cgataagacc agatgactcc gccagaggaa taaactgatc cggtgggata
     8821 attcccgttt gtggatgttc ccagcggaca agcacctcac agcccgtcag taccccagtc
     8881 tgcgcgcaga aaaccggttg gatccacggt ttgaattcgt ggttttccaa cgccagactg
     8941 atggcttcag gcgtgacgat atggtgatag tgattatcat taatatatct gtgactatta
     9001 ctccccgtgg cggatatgat ctccggattg tactgaaaga aaatatcgcg ccagaaagta
     9061 atatcgctct gaatccccag tttctcaaag agctgtttct tttgatgtga aattgtcttt
     9121 gcactgcgat ttctgtactg tgatatttct gttacagaca ttccctgcat cagcagcttg
     9181 agtagcagtc gttctttctc ggatacgcta ccaggcaatg acaaatgaac cgttgtaccg
     9241 aactcttgca gaaaaacacg aaaatcacat gaattcatga acacaccttt atcttttatc
     9301 gctgttcagt atgagtgatt acctgtcggt tcctgagcac acataagggg taaccataaa
     9361 catcagcgat gccatacgta taacatccca catcactatc aagaagatta tctttacctc
     9421 tgatgctcgc tcaaaaacag ttacgctaaa taccttaaaa aggtaatcac aagtcaaact
     9481 caggagataa tcacgcaaga atgcaatatt aaaattcaga tactcatttt tggtaataaa
     9541 gaagtagttg gactatttca atatttataa tttcccacta catactggca tccattcatg
     9601 agcaaaggcg cattatatga atttaacaat ccagatcaac tgaaaatacc tctccctcat
     9661 aaacacatag cgtcaacatt caatgacata atgagtaaag atgttggtta tacatacgta
     9721 tcattactct atgcctgtcc cttaaaaacc ccactcatta agactgaatc cattcagcaa
     9781 atgataacca atattatagc aatgccaata aaaacaatat ttaaaacatt caataaaata
     9841 tcacacacag ataattaaac cttaaataac aaatggttgc gttaatttca gttgatttac
     9901 gatacacatt tctcatgaat aatgcgtcat ttcaaatgat cagaaatatt tacattaaat
     9961 aagactaaac ttattgaaca ttcgcaacag cccaaatgta ggttttgggc tgcaatagtt
    10021 cttttctttt ttattatctg ttgtctggta atcatcaata gatactaagg gcttaaccgt
    10081 atagatataa cgtatcaaga ggtaggagaa acagcgccat atgcagacat ggcgcggttt
    10141 tatgcaataa caatcacgcg tccgggaaca tcacgctatg gcccggcgct tcacgatgga
    10201 gatgaataaa gttaaggtgc cgctcgtact ggtcaagaat atcggtgatc acctgctctt
    10261 tgctgtagtc catcaggtcg ttgccctggc tgccttctaa caggaaggtt tccagccggt
    10321 agtaggtcga tttaccgctg cgtgcgcggt aggtaaagcc cggcaccgaa tattgctgcg
    10381 gccaaatctg atagacaaag ttttgctctt cgcccatatg caccaacaaa tccagatgcc
    10441 ccaactgttg tccctcttcc ggcggcaggc tttttagctc cacgtacgcg ccgcgcaacc
    10501 gcagctcctg cgccacttct tccattgccg ggtaacagac cgtctccatc atctgtttag
    10561 tgtaacgcgt gcccggataa ttcatcaggc gcgagagacg ttttttccag ctcaggcgat
    10621 cctgaagccc cagcggtcgc ggcgcggtat cgcggttggc actttcacgg cggtaatctt
    10681 ctaccttcag agatttatac aaccccgcca tcacgaagaa aatcacaaag ctgaacggca
    10741 gccccatgat taccgtggtg ttttgcagcg cggatatccc gttggtcatc agcatgccga
    10801 gcgtcagcag accaatcgcc accgaccaga agacgcgcag ccagccgggg gcgtcgctgt
    10861 tgatatcttt aagctgcgag gtgaaattcc ccagcaccag cgcaccggag tccgccgagg
    10921 tcacataaaa cagcaggcca gtaatggtgg cgacggaggc gctaaaggta aacgccggat
    10981 actgcgccag caggctgtag aagccgcgct ccggatggac catcgcttcc tcggcaaatg
    11041 ccgcgccgcc gtggataatt tcatacagcg cgctattgcc gaacaccgag agccataaca
    11101 gcgtgaaggt aaacggaata atcaacgtgc ccagcacgaa ctggcgaatg gtacgcccac
    11161 gcgagatacg cgcaaggaac aagccgacaa acggcgacca tgccacccac catgcccaga
    11221 agaagagcgt ccagttattc atccactcaa ctggacggtc gaaggcaaaa ctgttgagcg
    11281 tcatgcccat aaagcgattc acatagtcgc caacattcag caccagcgca ttaagcagga
    11341 acgaagtgtc gcccataaac aatacgaaca ggatcaatcc cagcgccagc gcgacattaa
    11401 gctccgataa cacgcgaatg cccttatcga caccggaggt gacagagatc gtggcgatta
    11461 tcaccgacaa ggcgatcagt gccgcttttg ccgccatcga atcgggaata tcaaacagta
    11521 cgctcaagcc atagttaagc tgcaccacac cgataccgag cgtagtggca ataccgaaga
    11581 tagtgccgat caccgctgca atatccactg agtgacctat aggtccgtta atccgtttac
    11641 cgaagatcgg gtacagcgcc gagcggatgg tgagcggcaa attataacga tagctaaagt
    11701 atccgagcgc catgcccatc agcgcataca tcgaccagcc ggttaagccg tagtgaaaca
    11761 gcgtccagac catcgcctga cgcgcggcct caatcgtctg tcccgcgcct tccggcggct
    11821 gcatatactg cgttaccggt tcggctacgg aaaagaacat caggtcgata ccgatcccgg
    11881 cagcaaacag catcgccgcc caactcagca ggctgaattc cggtttggat tgttctggcc
    11941 cgagcttcac cgaaccaaaa cgcgaacaag cgatacagac cacaaagaca atatagagcg
    12001 ttgccgccag cagatagtac caaccgaagg ttttagacac ccagtccagc gtgcggccaa
    12061 tccacagggc cgagaagtcg cgaaacagga tcgttgtcag ggaaaacaac aaaatcagtc
    12121 cggcggaggt gtaaaacacc accggattga ttttgtcctt ttccctgctg tgtgaaaggt
    12181 ctgtcatcca gtatccccac tgttattgtt accatttaaa atcaaattcg taacaattaa
    12241 gacacatttt atattgaacg tccaatcaat aaccgcttta atagataaac aacgctgatg
    12301 aatggagtgg cgaaaatgcc caaattgggg atgcagtcga tccggcgcag acaactgatc
    12361 gacgccacac tggaagcaat aaatgaagtg ggcatgcacg atgcaacgat cgcgcagatc
    12421 gcccgccgtg caggcgtttc tacggggatc atcagccact atttcaggga caaaaatggt
    12481 ctgctggaag caaccatgcg cgacatcacc agtcagttac gtgacgcggt tttgaatcga
    12541 ttacatgcac ttccgcaggg cagtgcagag cagcgattac aggcgattgt tgccggaaac
    12601 ttcgatgaaa ctcaggtgag cagtgcggcg atgaaagcct ggctggcgtt ctgggccagc
    12661 agtatgcatc agccgatgct ctatcgttta cagcaggtca gcagccgccg cttgctgtcg
    12721 aatctggtga gcgagtttcg ccgcgaattg ccgcgcgaac aggcacagga agcgggctac
    12781 ggccttgccg cgctgattga cggattatgg ctgcgcgcgg cactgagcgg caaaccgctg
    12841 gataaacctc tcgcacactc gctgacccgt cactttatta ctcagcattt acccaccgat
    12901 taaccgagga gacgtgatgt cccgaatggc agaacagcag ctttatatac atggtggtta
    12961 tacctccgcc accagcggtc gcaccttcga gaccattaac ccggccaacg gtaacgtgct
    13021 ggcgaccgtg caggccgccg ggcgcgagga tgtcgatcgc gccgtgaaaa gcgccctgca
    13081 ggggcaaaaa atctgggcgt cgatgaccgc catggagcgc tcgcgtattc tgcgtcgggc
    13141 cgttgatatt ctgcgtgaac gcaatgacga actcgcaaaa ctggaaaccc tcgacaccgg
    13201 aaaagcatat tcggaaacct caaccgtcga tatcgttacc ggtgcggacg tgctggagta
    13261 ctacgccggg ctgatcccgg cgctggaagg cagccagatc ccgttgcgtg aaacgtcctt
    13321 tgtgtatacc cgccgcgaac cgctgggcgt agtggcaggg attggcgcat ggaactaccc
    13381 gatccagatt gccctgtgga aatccgcccc ggcgctggcg gcaggcaacg caatgatttt
    13441 caaaccgagc gaagttaccc cgcttaccgc gttaaagctg gctgaaattt acagcgaagc
    13501 gggcctgccg gacggcgtat ttaacgtgtt gccgggcgtg ggcgcggaga ccgggcaata
    13561 tctgaccgag catccgggca ttgccaaagt gtcatttacc ggcggtgtcg ccagcggcaa
    13621 aaaagtgatg gctaactcgg cggcctcttc cctgaaagaa gtgaccatgg aactgggcgg
    13681 taaatcaccg ctgatcgttt tcgatgatgc ggatctcgat ctcgccgccg atatcgccat
    13741 gatggcaaac ttcttcagct ccggtcaggt gtgtaccaat ggcacccgcg tcttcgttcc
    13801 ggcgaaatgc aaagccgcat ttgagcagaa aattctggcg cgcgttgagc gcattcgcgc
    13861 gggcgacgtt ttcgatccgc aaactaactt cggcccgctg gtcagcttcc cgcatcgcga
    13921 taacgtgctg cgctatatcg ccaaaggcaa agaggaaggc gcgcgcgtac tgtgcggcgg
    13981 cgatgtactg aaaggcgatg gcttcgataa cggcgcatgg gttgcaccga cagtgttcac
    14041 cgattgcagc gacgatatga ccatcgtgcg tgaagagatc ttcgggccag tgatgtccat
    14101 tctgacctac gagtcggaag acgaagtcat tcgccgcgct aacgataccg actacggcct
    14161 ggcggcgggc atcgtgacag cggacctgaa ccgcgcgcat cgcgtcattc atcagctgga
    14221 agcgggtatt tgctggatca acacctgggg cgaatccccg gcagagatgc ccgttggcgg
    14281 ctacaaacac tccggcattg gtcgcgagaa cggcgtgatg acgctccaga gttacaccca
    14341 ggtgaagtcc atccaggttg agatggctaa attccagtcc atattctaac caggaggttt
    14401 atttgcaatt tgactacatc attattggtg ccggctcagc cggcaacgtt ctcgctaccc
    14461 gtctgactga agatccgaat acctccgtgc tgctgcttga agcgggcggc ccggactatc
    14521 gctttgactt ccgcacccag atgcccgctg ccctggcatt cccgctacag ggtaaacgct
    14581 acaactgggc ctatgaaacg gaacctgaac cgtttatgaa taaccgccgc atggagtgcg
    14641 gacgcggtaa aggtctgggt ggatcgtcgc tgatcaacgg catgtgctac atccgtggca
    14701 atgcgctgga tctcgataac tgggcgcaag aacccggtct ggagaactgg agctacctcg
    14761 actgcctgcc ctactaccgc aaggccgaga ctcgcgatat gggtgaaaac gactatcacg
    14821 gcggtgatgg cccggtgagc gtcactacct ccaaacccgg cgtcaatccg ctgtttgaag
    14881 cgatgattga agcgggcgtg caggcgggct acccgcgcac ggacgatctc aacggttatc
    14941 agcaggaagg ttttggtccg atggatcgca ccgtcacgcc gcagggccgt cgcgccagca
    15001 ccgcgcgtgg ctatctcgat caggccaaat cgcgtcctaa cctgaccatt cgtactcacg
    15061 ctatgaccga tcacatcatt tttgacggca aacgcgcggt gggcgtcgaa tggctggaag
    15121 gcgacagcac catcccaacc cgcgcaacgg ccaacaaaga agtgctgtta tgtgcaggcg
    15181 cgattgcctc accgcagatc ctgcaacgct ccggcgtcgg caacgctgaa ctgctggcgg
    15241 agtttgatat tccgctggtg catgaattac ccggcgtcgg cgaaaatctt caggatcatc
    15301 tggagatgta tctgcaatat gagtgcaaag aaccggtttc cctctaccct gccctgcagt
    15361 ggtggaacca gccgaaaatc ggtgcggagt ggctgtttgg cggcactggc gttggtgcca
    15421 gcaaccactt tgaagcaggt ggatttattc gcagccgtga ggaatttgcg tggccgaata
    15481 ttcagtacca tttcctgcca gtagcgatta actataacgg ctcgaatgca gtgaaagagc
    15541 acggtttcca gtgccacgtc ggctcaatgc gctcgccaag ccgtgggcat gtgcggatta
    15601 aatcccgcga cccgcaccag catccggcga ttctgtttaa ctacatgtcg cacgagcagg
    15661 actggcagga gttccgcgac gcaattcgca tcacccgcga gatcatgcat caacccgcgc
    15721 tggatcagta tcgtggccgc gaaatcagcc ccggtgtcga atgccagacg gatgaacagc
    15781 tcgatgagtt cgtgcgtaac cacgccgaaa ccgccttcca tccgtgcggt acctgcaaaa
    15841 tgggttacga cgagatgtcc gtggttgacg gcgaaggccg cgtacacggg ttagaaggcc
    15901 tgcgtgtggt ggatgcgtcg attatgccgc agattatcac cgggaatttg aacgccacga
    15961 caattatgat tggcgagaaa atagcggata tgattcgtgg acaggaagcg ctgccgagga
    16021 gcacggcggg atattttgtg gcaaatggga tgccggtgag agcgaaaaaa tgagtcgtga
    16081 tgtgaactaa cgcaggaacc gatcagattg agagttaccg ttccagagag ggggaccgaa
    16141 tccttatata aacactgagg taactctcat gcttcatatc cagtattcat gatgcgggct
    16201 ttttgctgat atttaacccg aacattcctg atacagacta ttaatacaac cttatctgcg
    16261 acagatttta ttttctggat atattatgcg tgaacaaatc aaacaggata tcgatctgat
    16321 tgagatttta ttttatctga agaaaaagat tcgtgttatc ctttttatta tggctatatg
    16381 tatggctatg gtgctgttgt ttctgtatat caataaagac aatataaaag tgatttacag
    16441 cctaaaaata aaccagacaa cgccaggtat acttgttagc tgtgatagca ataataattt
    16501 tgcctgtcag actacaatga ctgaagatgt tattcagcga attactacat tttttcacac
    16561 cagcccagat gtcaagaaca gagaaataag gctggaatgg tcaggagata agagagcttt
    16621 accaactgct gaagaggaaa tatctcgcgt gcaggcctct attatcaaat ggtatgcgtc
    16681 agaatatcat aatggcaggc aagttctcga tgagatacaa acgccttcag caattaacag
    16741 tgagctttat acaaaaatga tatacctgac caggaactgg tcattgtatc cgaacggtga
    16801 tggctgtgta actataagct caccagaaat aaaaaataaa taccctgctg ccatttgcct
    16861 ggctctggga ttttttctaa gcattgtaat ttctgtaatg ttttgccttg tcaaaaaaat
    16921 ggtagatgaa taccaacaaa actctgggca gtaataaaca acattctgat agagagtgcc
    16981 tgtataaagc tgaagtgaca atgaccggga agggaacccg ttttcaggaa tggatgcctg
    17041 tctttttcac tgtaaatatc ctggcattgg taataccagg atatttacag aagagcagca
    17101 taaccatgac ggaacggggt taagtcaggt tgtcctaaac gctattttca acctcgtatg
    17161 ccttcttcag gtttatgtcc agacttcata tctctctcaa caatcctcaa taatcagata
    17221 caccgctttc accgggccat gaactccgac gactttgata agctcaatat ccgccgttga
    17281 actggggccg ctaatgatgt taatgcagga aggcattcgt tcaccggcct gcgctttctg
    17341 atgcaatttt tctgcgagtt gcgctacacg cggcaggata gtgcttttac gcaggataaa
    17401 aagagaatat tccgggagca ggctcaatga acgcccgcgc tcggcggcgg aaaaaagaat
    17461 cacgcctccc gattcggtta aaccatattc agcatacaca acacccactt tagcctgctc
    17521 tgcctgcgag atattctcgg cacctttcgc cggatcccaa acaacggcat tgcattcctg
    17581 ctgcaaacgt tcgctaatcc ccaattcctc cagcctcgtg tcaccgctaa tcacgaccga
    17641 ctgatctccc agctctttac acagacgtat tgcagcttct gccgccttcg cctcgctggt
    17701 cagctcacag cgcgtcaaca taacatcgct ggcaaactga ataaacgcgt cacagcgctg
    17761 ctgttggtta agttgggtaa gccgctcgtt agcatagttg ttaagcggcg catcttctgc
    17821 ttgcggttca agtcgcagcg ggcgacccag tgcctgagca acgttattca aaaattcgcc
    17881 tcgattatcc attctttttc tcctgcgcct gatgtttctt aaaccaacta cggaaactct
    17941 ctccgtcagc ttcaggaaga tcgcgtgctt ccatccagtc gctaatcgcg ccaaatttga
    18001 gtggtgtttt gccgccattg ataaaccagc ttgccgcatg agcaccggcc atcatcccga
    18061 ctttccacaa tcctggatga ctattggcat aagcgaacat ttttatcgcc cgttgctctg
    18121 cttttgcggt gatccctttt tcagccatca cccgacgatg acgcaaaatc agttttgaca
    18181 gcggaatacg caccggacac acgttgtcac aagctgtgca taaagagcag gcgtagggta
    18241 aatctttaaa atctttatag ccgccaagta gcggagaaat caccgcacca attggccctg
    18301 gataaataga gccatatcca tgaccgccaa tatggcgata tgccggacaa gtattcatac
    18361 aagccccgca gcgaatacag cgcagcacat cccgaaattc agaggccagc acctcagaac
    18421 gcccgttatc gacaataacc agatgaaact cttcaggacc atcaacgtgc ccagcttcgc
    18481 gcggtcctgt cagccaggtg ttgtatcccg tcaaacgtgc accaacggca ctgcgcgcca
    18541 gcatggtgat caatacatct acctcggcaa acgtgggggc aatacgctcc attcccatca
    18601 ctgcaatatg cgttttaggc agcgtggtac acattcgcgc attaccttca ttggtcacca
    18661 ggcataccga accggtctct gccaccgcga aattacagcc ggtaatacct atttcagcac
    18721 tgaggaaatc ttcgcggatt ttttgccgga tgaataaggt catcgcttca ggcgtttccg
    18781 gcccctcata gcccagacgt tcgtgtagca ctcgacggat ctgatggcga tctttatgaa
    18841 ttgccgggac cacaacatga gatggcggat c
//
LOCUS       DAMZET010000082        18328 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.082, whole genome shotgun sequence.
ACCESSION   DAMZET010000082 DAMZET010000000
VERSION     DAMZET010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.082"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..257)
                     /locus_tag="POB34_002832"
     CDS             complement(<1..257)
                     /locus_tag="POB34_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919742.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVEAGCQQLERGDVLPHDMVESSAAAWRDEMSRKV"
     gene            complement(312..470)
                     /locus_tag="POB34_002833"
     CDS             complement(312..470)
                     /locus_tag="POB34_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001700046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919743.1"
                     /translation="MAESGYGLAGLGMGKVKSINQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGISW"
     gene            complement(1166..2197)
                     /locus_tag="POB34_002834"
     CDS             complement(1166..2197)
                     /locus_tag="POB34_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDI5919744.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATTFLVEPERK
                     RTKRRRGEHSTKPKCENPSWYRPERYKALSGQLGHAYNRLVKKDPVTGEQSLRMHMSL
                     HPFYVQKRTYVGRKYAFRPEKQRLLDAVWPVLVSFSDAGTHTVGMSVSRLAREISPKD
                     SKGKVIPELEVTVSRLSRLLAEQVRFGVLGVSEETLWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRMAIRELRQLE
                     LTLAAPPPH"
     gene            3170..3433
                     /locus_tag="POB34_002835"
     CDS             3170..3433
                     /locus_tag="POB34_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HDI5919745.1"
                     /translation="MPYHKDRNLYFLSSSMVAQAGQALAWPVSDDAGIPTPVWAITNE
                     RRNSVIAFLVVIGDLCYVTSTDTETPGHHSVGRHVSRAINKPA"
     gene            3351..3638
                     /locus_tag="POB34_002836"
     CDS             3351..3638
                     /locus_tag="POB34_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HDI5919746.1"
                     /translation="MSPQLIQKLPAITLLEGMFPELSTNQLKVCVFYAMGVPYDAIAQ
                     NCRLSPETVRTYLKRSLKNLNLEGYDALRSAVLMRTFVFMISNTAKENEKM"
     gene            4080..4613
                     /locus_tag="POB34_002837"
     CDS             4080..4613
                     /locus_tag="POB34_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /protein_id="HDI5919747.1"
                     /translation="MNIEHLNNRNWYLAQYNTAGKNRESLFSWLNEQNVVPWTPLITR
                     KIRRADSRCCYRERIFAIFPGYFFILANFDIHPVSALRRHSAFIDFVKFGGEIKPVNK
                     DIVDGLMKIYPDPVLNPGAREELNAASSIWLTKAQYQYLLRMENTLQPESRISLLLEL
                     VSNAEHHGFMERLVNIP"
     gene            4867..5550
                     /locus_tag="POB34_002838"
     CDS             4867..5550
                     /locus_tag="POB34_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /protein_id="HDI5919748.1"
                     /translation="MTTTEHPVRQLLWCALNALKTAQENHNITSETGLRHYLLEWLSG
                     ASRHPEFRSLPGEIMALKALTEKDRNIPIIGTLNTLFLSSATVGECPLFRFRAALGKL
                     QKAGWRTYVCPWPERVFNESIERACSGKRHLLQLSRTEECFLPTGDMRAPVTLQLITP
                     LHRKTMQLLEAAELIFSDEGFQVVRGYEHQFGIERRETLFHTLFIGLPSLPEDVWGAT
                     QEQTTITLH"
     gene            5564..6130
                     /locus_tag="POB34_002839"
     CDS             5564..6130
                     /locus_tag="POB34_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HDI5919749.1"
                     /translation="MKLQYRIPLAIYSVWCIIAILLCKDGLYQMNIWTLVEILGMIAL
                     PLLVRPFFILLRIIFRKNSFKTKDMSDNHTVCVFLSALADTSSKKAIKKHFKQLLEKL
                     PPLLRQKKRIYMKSHLLTEARTQKLICSLRRKGLDVTAERRESAMNSVMFRILIVSRI
                     LSQWKVPHINPRCGIVILTLKNDSGNRE"
     gene            6398..6652
                     /locus_tag="POB34_002840"
     CDS             6398..6652
                     /locus_tag="POB34_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HDI5919750.1"
                     /translation="MPQQHPGRLQVLVVDTHCKRKLFSTKTQTDPDELARRFCTPDNC
                     LVVVLCNNRFLFRLERAPGSHCRWRKGSRSRHQHLQDWLS"
     gene            6695..7147
                     /locus_tag="POB34_002841"
     CDS             6695..7147
                     /locus_tag="POB34_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pili assembly chaperone"
                     /protein_id="HDI5919751.1"
                     /translation="MSFLFKLTIAILIVFAILFPILISSDNKLSALFWKIPKDFWVFL
                     IIMYGAIITVVFIESSEQARIRRQQYLQQYKMPNEIHENVVIKNSIISRETTDDESDI
                     NTLYLDSFKSKNNCKSFETNSNLLDKNGKPVEIMVCDVQELHFFVPKN"
     gene            7821..8888
                     /gene="pilL"
                     /locus_tag="POB34_002842"
     CDS             7821..8888
                     /gene="pilL"
                     /locus_tag="POB34_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis lipoprotein PilL"
                     /protein_id="HDI5919752.1"
                     /translation="MKKNYLTLILPSLLSGCAGISGSNNVAPSDTPALVFVDGQISES
                     AGVLALTQQRLSPPKITPPPRVSVPETVKTAHPVSESRPLPSPTISQNFSRLIITGKP
                     GSAPVTLSPTARNRTVSQWIKTLLPEGWKFRQENAATPKLNTKLVSWSANDQWTRSLN
                     RLLEEQHLWGHLDWDNKTLTVTTSAAAPAQDTATTISPAAMVTTSTSPESPTTANSQN
                     KPRNPFRGNSVSSSTPAATPTPTGSTVKSIPLMTGTPVKPVSQGKEWRAPAGTTLREN
                     IIKWAEETKCESMASTHWMVIWPLSVTDYRLDAPLMFRGSFESAMEQVFELYRTAQKP
                     LYAQASRMQCIITVSDTRDGR"
     gene            8888..9325
                     /gene="pilM"
                     /locus_tag="POB34_002843"
     CDS             8888..9325
                     /gene="pilM"
                     /locus_tag="POB34_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HDI5919753.1"
                     /translation="MGWLVMAVGLTILIITGSYQNQKMSETTNAQQYASASVWASQIL
                     MIANRINDIRYVSGQQDGVISSDKLALPVTPDSRIKHQLQQGRLWVWMPEQPGLVETL
                     RSKSRGSALIGIFQNGQLTWLSGTATGLTPPAGITAGSVVYVN"
     gene            9339..11021
                     /locus_tag="POB34_002844"
     CDS             9339..11021
                     /locus_tag="POB34_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HDI5919754.1"
                     /translation="MKKSHQRSMKLAVLPCMIAVALSISGCTFSEINKMQKKAQEDSA
                     HAREKVSALSARKSQALTWLDNQWINPVPVAQVSREKKQTAPACYITQARKGEITLQE
                     LGQRITAVCGIPVIITPDAANSTLEGGATRQMTGTLPAPDENGRLPLSSLGSTTMTTS
                     TQPLTLNNLMWQGDINGLLDLMASRSGLYWRMDNGRIVFYLTETRTYPLHMLNTKTSS
                     SSSVSSGSTSTMGATGGQDNSASGDATSSQSTTVGQEYDLYEDIRKTIEAMLTPEKGR
                     YWLSASSSTLTVTDTPAVQEAVARYVDEQNSIMNRQVALNVQVLSVSNTRNEQFGLDW
                     NLVYKSLHSAGATLNNASGDFTGATSAGVSILDTATGNAAKFSGSSLLIKALSEQGDV
                     SVVTSQESTVTNLTPVPIQMADQTVYVAQSATTTTTDVGATTTLTPGMITTGFNMTLL
                     PLIQKTGNLQLQMNFNLSDPPTIRSFTSKDGNSYIEMPYTKLRSLSQKVNLKEGQSLV
                     VTGFDQNNTTTSKAGTFTPANPLFGGSQTGKNERSTLVIIITPTFPSGGNNG"
     gene            11014..12309
                     /gene="pilO2"
                     /locus_tag="POB34_002845"
     CDS             11014..12309
                     /gene="pilO2"
                     /locus_tag="POB34_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HDI5919755.1"
                     /translation="MADEDINPVVLLADPKVNHRVWAACLKWSPVVKKQRVPSHQKHK
                     PHVKSRRLTSLKVTVGSRSSRGKISRITGTGILARPERNHYFSLALAFCSWVRNGYGV
                     FRYSDKELLFLASINGQPAVMADLSGNDADVAQKVSLFLTMNEEPPEKWQVVSPLEHP
                     DNWESIITRLSSADLRRCKLTVGNRSKFTLPAVLFLVAASAGTVFWMTQPEPDVVPTP
                     EEIAARARLQFKKPDPPPELPHPWASQPVISDFLKACADLRKPSPVALEGWKLTGGTC
                     TPETFTLIYERQPGGTIEGFLARSKEIFNVIPDFNLKDGARLASVTRPLPSLPRRDEA
                     VPTPSEQLMRVFTWFQKKQLTPAINEIAIPEPLPGNDGEPAPVQKWKEYQFSLSTPVN
                     PDELFPLFQDTGVRLSNIHFELNGGTFSYSSEGHIYASR"
     gene            12296..12748
                     /gene="pilP"
                     /locus_tag="POB34_002846"
     CDS             12296..12748
                     /gene="pilP"
                     /locus_tag="POB34_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HDI5919756.1"
                     /translation="MRPGKLLIIPSIFFFSGFSFATTQPLVTIGELEAQQNRNILLQA
                     KVQGAQLQKQLEESDVTSSSETVSGFSGVTASLPSVSEQPTSKNRKELPVIMEINGKD
                     KRLNAVLRMADGRQTSVTTGSQLPGTSVTVKSISLSGVTLSDGTPLTF"
     gene            12759..14312
                     /gene="tadA"
                     /locus_tag="POB34_002847"
     CDS             12759..14312
                     /gene="tadA"
                     /locus_tag="POB34_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDI5919757.1"
                     /translation="MDKYNLKDALLFISRGDTHEILIETNQRTRPDVQSNLQELLKLY
                     PDINPKVVSLSELQEAGQDEKNRGPKERIIHLKDLADVSESEKKVLSYFETARKLGAS
                     DIHFLISESIFKVRMRIFGELQTVDEDQPALGYSLCATAILSMADVTETSFFPQREQD
                     ARLSPQLMRKIGIFGARYSHRPTGDGLIAVMRLIPDDGDKVPTFKQLGFIPEQIRLLN
                     IMLRRPEGKIVLSGPTGSGKSTTLRSACRVYLDDNQGRHLLTIEDPLEGQILGATQTP
                     IICDKSDEDAVKLAWSRAISSAMRLDPDAIMEGEMRDLISMMSTTYAAQTGHIVLTTL
                     HTNSALGIPERMITMGMNADLICDAQLLIGMISQRLVPTLCPSCRIPWEKRAPELSDD
                     ERDYLERHCNKDSLCSTDNIWFRNHHGCSECNHDVIINGRKRGEIGKGLTGRTVIAEV
                     IEPDNRLFQILKTRGKVAARKYWLENMKGISRVEHLLRRINEGLVDPLEADRIIPLDE
                     DERLSIDDV"
     gene            14325..15422
                     /locus_tag="POB34_002848"
     CDS             14325..15422
                     /locus_tag="POB34_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001699973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDI5919758.1"
                     /translation="MREMNFSQRLRRFIVRKTFSAPYRVQFYEALRFLLENKQPLKTA
                     LEQMRDAWTDFGRKWHPFAELATDCIESLRENSGENSLEYTLSLWVPQEEAAVISAGI
                     RSGSIVDALQFATTLTDAKEQIHQAIWQMAIYPVGLLIMMTGTLYVLNTELIPELSKI
                     SSPDSWSGALGFLYGLSVFVDNYGAICAVLFAVITGLISWSLPNWKSPDSVRTFADKI
                     MPWSIYQDIQGATFLLNMAALLKAKMTTLNSLNILQEFASPWLSTRLDSIIYRVRQGD
                     HLGLALRQCGYQFPSREAANFLSLLQGDGATELISNYGQRWLSQTLQRVKKRANVIRL
                     IMLIFLVMSLMLLVFAIMDIQSISDNSMGNF"
     gene            15440..16054
                     /locus_tag="POB34_002849"
     CDS             15440..16054
                     /locus_tag="POB34_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilX"
                     /protein_id="HDI5919759.1"
                     /translation="MLVENINTTLTGNNKKNEPHDKGWAILEQGTIALVVLFVIVVVL
                     GSLYALRTRANVATETANIQTIITSAQSLLKGSDGYTFTSSAKMTGALIQMGVIPSGM
                     TVQGDKTSGTATLYNAWGGAVTVAPASTSGFNNGFTVTYDKVPQDACIQIATRISKTG
                     LTNGITLNSTAHSDGKVTTEEASTQCKADNGSTGTNKLIFTING"
     gene            16064..16624
                     /locus_tag="POB34_002850"
     CDS             16064..16624
                     /locus_tag="POB34_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDI5919760.1"
                     /translation="MSGGTVPLRLISLILLFSLIPPARASYVAPKQIWTNKWDNCFAA
                     AGARYQIEPLLLKAISAGESSLRPGAININKDKKTGKASSTDYGLMQINSTHIPKLIN
                     MGVIKKSEDLITKPCLNIHIGSWILARHFQICGVSWNCLGSYNAGFRKDRHETREQYA
                     NKIWRIYRDMKGICLPGQGGRQCRQS"
     gene            16609..17265
                     /locus_tag="POB34_002851"
     CDS             16609..17265
                     /locus_tag="POB34_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDI5919761.1"
                     /translation="MQTIVGISLSPVYILPLMFTFSIIGRTLLFRVRYFLSDTGHLWY
                     KTHPAVLSGIWLYSIAVALIILSSSPLLYRIHAVLILSFILQMAVTDALTGLLPGTFT
                     RRFLIAGMLSQITTDIWWFRTTEFATAAIVLFCLHKLVNRHRLNIGTGDLWLIAGITA
                     WSGLYNAIWCVLLGTGGFVLWHSTWCIKGHKEGPLGPWLCFGHVLLLLDNLYQPLWVI
                     "
     gene            17265..>18328
                     /gene="pilV"
                     /locus_tag="POB34_002852"
     CDS             17265..>18328
                     /gene="pilV"
                     /locus_tag="POB34_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HDI5919762.1"
                     /translation="MKKYDRGWASLETGAALLIVMLLIAWGAGIWQDYIQTKGWQTEA
                     RLVSNWTSAARSYIGKNYTTLQGSSTTTTPAVITTTMLKNTGFLSSGFTETNSEGQRL
                     QAYVVRNAQNPELLQAMVVSSGGTPYPVKALIQMAKDITTGLGGYIQDGKTATGALRS
                     WSVALSNYGAKSGNGHIAVLLSTDELSGAAEDTDRLYRFQVNGRPDLNKMHTAIDMGS
                     NNLNNVGAVNAQTGNFSGNVNGVNGTFSGQVKGNSGNFDVNVTAGGDIRSNNGWLITR
                     NSKGWLNETHGGGFYMSDGSWVRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLERT
                     AVAGASCSPNGLVGRDNTGA"
BASE COUNT         5020 a         4410 c         4193 g         4705 t
ORIGIN      
        1 actttcctgc tcatttcatc ccgccatgca gcagcggaag attcgaccat gtcatgggga
       61 agcacatcgc cacgctcgag ttgctgacat cctgcctcaa cctggtcacg gaaccaggta
      121 ttatgctgcc gttccacggt ctggcgcata aaatcccgga ttaactgaga gccattacgg
      181 tccatgcttt ttgcagcggc cataaaagca tccttcagtt cagcgtcgat tcttaaactc
      241 atattaacct gtgccattta tcacctcgtc attactacgt atatacacag tatataacga
      301 ttcagatatc gtcaccagga tatgccgcgc cagcggcatg gaaggcggca cgctgtggtt
      361 acatgtgata ccggagtaaa accgccgaag cccggcgtca gccggtactg attgatagat
      421 ttcaccttac ccatccccag ccctgctaag ccatacccgc tttcagccat gagagagttt
      481 ctgtgcgcgg tcggagtggt cccgacgagg gtttacccga agtcggggcg tatctccgcg
      541 ttagcgggcc gtcagggccg cttacgagcg tgtactgaga acatccagcc agaagactga
      601 cagcgatgat aatgtagtta caacattcat aattaaaagc gatgttgttc ccgccctttg
      661 ggcgggggcg gcctcgcttt tcagttatga ggggtgggca cggtgctgcg tttgtgtggt
      721 gtttttattc ctgatgtctt tttgttgtgg tttcagaacg gtgaggttcg gggcccgcgc
      781 gttagcgtgg gaggtgcttt tagattctga gcggcgcggc cttggccgct gcaagcgaag
      841 gcctctttgt atttgttcac agtggtctca gagataaaga ttttccctgc ggcgaagcaa
      901 cgcaggggat taaataatgg ggttaagact atattgcagg tttgctgttt caggtttgca
      961 gcttcttaaa gttctttgtt tgcggattta tgtggataac tgaaacaacg tggccatgtt
     1021 ctgatgactg atgcgtggtt ttatgggcag atatgcccct ctgaggggat ttaaggtgat
     1081 tactgcggat tttgggctga gcggaaccag cgccgaaagc cccggagggg ctggtgatga
     1141 ttcagtgctg agggaatggt gctgtctagt gcggtggcgg ggcagccagc gtcagttcaa
     1201 gctgacgcaa ctccctgatg gccattcgct tcagatggtc atcggaacaa aaataggctt
     1261 catccggagg cagacgcttc ctgagatact ctgccatctc atgaatctgc tggtcggcag
     1321 gcagtttctt caggcgtctg gcgcgcttac tggctgctgc tttcgcacga cggtgtttca
     1381 gcgcatcctg actgcgctgc agataccagc gttttctggc cgcatgtacg gagatatctt
     1441 catcctcacg cagaacacct tcccgaatga gctgctggcg gatttcactt tcacgcagtc
     1501 gtttctgctg ctgttcgtga agttttacca tgtcgacgcc cagcatctgc cagcctgccg
     1561 gtgttatcca gacgtaacgt ggcagacgct ggcgggtttc acggtcccac agggtttcct
     1621 ctgaaacacc cagcacacca aaacgtacct gttcggccag caaacgggaa aggcgggaga
     1681 ccgtcacttc cagttccgga ataaccttcc ccttgctgtc tttcgggctg atttctctgg
     1741 ccagacggga aacactcatg cctacggtat gtgtgcccgc atcactgaag ctgaccagaa
     1801 ccggccagac ggcatcgagg aggcgttgtt tttccggacg gaaagcatat ttgcgaccga
     1861 catacgttcg tttctgcacg taaaaaggat gcagagacat gtgcatgcgc aggctctgct
     1921 cgccggtcac cgggtccttt ttcaccagac ggttgtaggc gtgcccgagc tgcccgctca
     1981 gcgccttata gcgctcagga cgataccagg acggattttc gcatttgggt ttggtggagt
     2041 gctcaccacg acgacgcttc gtgcgcttcc tttccggttc aaccaggaaa gtggtcgccc
     2101 ttcccgcttc atagtcttcc cagaaacgga tctgctcttc gggtgctaac tgactccagt
     2161 gtactgcgtt atatggagta ttcttcagac ctgccacaag ctgtcgctta tggcggagcg
     2221 attgtgccgt gtatttatac actgcacagg gttgaatctc tggtacggtt tcattatact
     2281 ttacccgtat tctgtgaggc ccccactatc tttcgtcgtt ccgccaagtt cgtaagaaag
     2341 atagtggggg ttttcctttt atggcttccg gaacatcttc agtcttctga cttactgccc
     2401 gtacatgcca tctctcctga atgccttatg gcgcattctg ccgtccggct ttcgccgctt
     2461 tgctccacag agtgccaccc tctgcacagc taataacagc cgaaatatac ccgctttacg
     2521 ttaatttgaa aagcattaca acgatctctt ctcttccagt gcctcctcac tgatacctct
     2581 aataagttaa tcttgacctg cgtcaaaaaa ataaaatcta tttttaaaag cagatttttc
     2641 tgctttagat ctttcttttg gattaaaaga tcgactttta tcctactcac gatcatttat
     2701 cttacaaccg atctacctaa cagaataata ttctgtttta atgcaattaa acgatctaaa
     2761 taacctttaa aaaatcattt tgtagatcat tcgatcgtaa atagaatgat tcttagatga
     2821 taaaaccatt tgttacacaa cccgccaatt gtgtaacaac agcctctaca agagcatatt
     2881 tgttcatatg ttcgtcagaa ctgcggattg tctggacgag ggtcagacac cgtttcgtgc
     2941 atatggacca gcatgttcga acgccacaaa gcagaagcat catgcctgga tcgcttcggc
     3001 gcggagcgtt aagttttgac gcttttttgt atgtttttta agctgttagt tgactcggtg
     3061 cgttatacta agtacccttt actggtcaca ggcaaaactg tgatcgggcg taggaacccg
     3121 cgtagtcatc gtcggcgcag aagttaattt cttcatttgg cgtcgttttt tgccatacca
     3181 taaagacagg aacttgtatt tcttgtcgtc atctatggta gctcaggcgg ggcaggcatt
     3241 agcctggccg gtttccgatg acgccggtat tcctaccccc gtctgggcta tcaccaatga
     3301 gcgtaggaac tcggtgatag cgtttttagt tgtcatcgga gacctttgct atgtcacctc
     3361 aactgataca gaaactcccg gccatcactc tgttggaagg catgtttccc gagctatcaa
     3421 caaaccagct taaagtctgc gttttttacg caatgggtgt tccttacgac gccattgctc
     3481 agaactgtcg gctttctccc gaaacggtac gaacctacct gaagcgaagc ctgaagaatt
     3541 taaatctgga aggatatgat gcacttcgct ctgctgtgct gatgcgaact ttcgttttca
     3601 tgattagcaa tacagctaaa gaaaacgaaa aaatgtgaac aggtgtcccc attaaagggg
     3661 acactttcat ggaaattgca ttttgcactt tacaggcgat cccaaattga taaacataaa
     3721 caccaagcac aagttacttt aataaagcaa atcacttcag acggtgtccc agttatttgt
     3781 atctggctca tgcccgatat atggcgacct tactctgaac tgacgtgctg atgttttatt
     3841 tcgcgtatca caatactgtg ccactgtatt gctgatatta gtgcttagcc gttctttatc
     3901 tgtgagttcg ccaaatcacc gatacagaac gtactagagt ctgctggtta tatgcctgtc
     3961 agaactgcag tttatccaga tatgggctgg acactgcttc agacatatga tttagcatat
     4021 tcgcctgaca tcgcaaaatc gtccatcatg gatcggtttt gcgcggagcg ttgaacttcg
     4081 tgaatataga acacctgaat aaccggaact ggtatcttgc gcaatataat acagccggca
     4141 aaaacagaga atctttattc tcatggctga atgagcaaaa cgtcgtacca tggactccat
     4201 taataacaag aaagataaga cgggcagact ccaggtgctg ttaccgtgaa agaatattcg
     4261 ctatttttcc agggtacttt tttattctgg ctaattttga cattcaccca gtatcagcat
     4321 tacgtcgaca tagcgccttc attgatttcg tgaagtttgg cggtgaaata aaacccgtca
     4381 ataaagatat tgttgatgga ttaatgaaga tttacccgga ccctgtgcta aatccgggag
     4441 ccagggaaga gctgaatgcg gcttccagca tatggctgac aaaagcacaa tatcagtatc
     4501 tcctcagaat ggaaaatact ctccagcctg agtcgaggat atccctgctc ctggaactgg
     4561 tatcaaatgc cgaacatcac ggctttatgg agcgccttgt aaacatccca tagccagtat
     4621 ttcccggcct tcgggccggg cttttttcaa ttcaagagaa tattccggag tgaagcctgc
     4681 acgaagccat ccccaccatg gactccccct gtcttttgat ggaaccgtgt atttcttaat
     4741 ggcgaaagaa ataaaaggct tcaccccaga gtattgattc gttttatcaa aggccggccc
     4801 gtgccatcac gtgtggcgga gagaacggat accggtcttc ttttcatctc acagtaggac
     4861 attcgcatga ccacgacaga acatcccgtg cgccagttgc tctggtgtgc actgaatgcc
     4921 ctgaaaacgg cacaggaaaa ccacaatata accagtgaaa ccggattacg gcattacctg
     4981 cttgaatggc tttctggtgc cagcagacat cctgagttcc gtagtctccc tggcgaaatc
     5041 atggcgttaa aagcattgac cgaaaaagat cgcaatattc ccatcatcgg aacgctgaat
     5101 acgctctttc tcagctctgc cactgtcggg gaatgtccgt tgtttcgttt tcgggctgca
     5161 ctgggtaaat tacagaaagc aggctggcgc acatatgtct gcccatggcc tgagagggtg
     5221 ttcaatgaat ccattgaacg ggcctgttcc gggaaacgtc atctgctaca actaagtcgc
     5281 accgaagagt gttttctacc gacaggcgat atgagggcac cagttacgtt gcagctgatc
     5341 actccactac accgcaaaac tatgcagctt ctggaagcgg cagagttaat attctccgac
     5401 gaaggatttc aggtggtgag aggatacgaa catcaattcg ggatcgaacg cagagaaacc
     5461 ctctttcaca ccttatttat cgggctgccc agcctgccag aagatgtctg gggtgcaaca
     5521 caggagcaga cgactatcac attacactga ggaagagcaa tacatgaaac tccaatatcg
     5581 tattccatta gctatatata gcgtctggtg cattatagcg attttgctct gcaaggatgg
     5641 cttgtaccag atgaacatat ggacgctagt agagatattg gggatgatag ctctcccgct
     5701 gttagtccgt ccgtttttca ttctgcttag aataatattt aggaaaaata gttttaaaac
     5761 caaagacatg tctgacaatc acacagtatg tgttttcctg agcgcactcg cagatacttc
     5821 ctcaaagaag gcgataaaaa aacacttcaa gcaattactc gaaaagctgc cccccttatt
     5881 acgccagaag aaacgtatct acatgaaatc ccatcttctg actgaagcca ggacacaaaa
     5941 gctgatttgc tctctgcgcc gaaaagggct ggatgtaaca gcggaacgaa gggaatccgc
     6001 catgaatagc gtcatgttcc gcatcctcat cgtcagcaga atcttatctc agtggaaagt
     6061 gcctcacata aatccccgct gtggtattgt cattctgacc ctgaaaaacg attctggaaa
     6121 cagggaataa cagttataag agcaccacga tttccgacag gaaaacatac atgaaaatca
     6181 acgtagatga ccctggcaaa aagagcttcg ctctcattac gcctcacgtg cgttaattcc
     6241 cgacacaccc cgtgacccgt cttcgcagcc tgtaccaccc tttctgccgt tcccgggatt
     6301 ctttctgtca ctactcgctt gtgccgtttt ttcgcaaccg gtacgtgcag ttacgacggt
     6361 acagctgctt cttttcccgt tccgaaagga gtctttgatg ccacagcaac atcccggacg
     6421 actacaggta ctggtcgtcg acacgcactg caaacgcaag ctgttttcaa cgaaaacaca
     6481 gacagatccc gatgagcttg cccgacgctt ctgcacgccg gataactgcc ttgtggtggt
     6541 actatgcaac aaccgatttc tgttccgcct ggaacgtgca cccggtagcc actgccgctg
     6601 gcgcaagggt agccgctccc gccatcagca cctgcaggac tggctgtcgt aaaaacaaca
     6661 aaaacaaaag acctcgtcaa aacaggaggg gaccatgtcg tttttattta aattaaccat
     6721 cgcgattttg attgtctttg caatcctttt tccgatttta atttcatcag ataacaaact
     6781 gtccgcactt ttttggaaga taccaaaaga tttctgggtt ttcctgatta tcatgtacgg
     6841 ggcaatcatc acggtggtgt tcatagaaag cagtgaacag gcacgcatcc gccgccaaca
     6901 atatttgcaa cagtacaaaa tgccaaatga gatacatgaa aatgtggtga taaaaaacag
     6961 tattatttca agagaaacaa cagatgacga atctgacatt aacaccctgt accttgattc
     7021 atttaaaagt aaaaacaact gcaaatcttt tgaaaccaat tcaaatttac tggataaaaa
     7081 tggtaagcct gtggaaataa tggtgtgtga tgttcaggaa ctgcattttt tcgttccaaa
     7141 gaattaaaca gttaatcccc cttactctgt tatcttagtc gccggcatca actaacatga
     7201 aaaataataa cgtaccatta tatctatggt tactttggtg tacgttttgc ctgtacatca
     7261 ctaccccgtg gcaatacttc cgttatctat acactgacca catatcacta tcttatatat
     7321 tatatttctg tatagtggtt attatctgct tttccccact acttttcaga tgcgtgcttc
     7381 ttatcgtaag gtctgtatct aaatacaact tcacattctt tgcgaaaaaa aatggcagta
     7441 aagaatcaag catacatctt agcccctatg ccgatacacg ttgcccggag tcaatgagga
     7501 aacactggga tgtgttactt tctctgatat caaaagcaat taataatgat gaaaccattg
     7561 ttatgcattc acatctcttt actcctgcaa gaataaataa acttataaag agactggaat
     7621 cttatggttt gaatttctct gttattacat gcccacggaa aacaagtctg tatgaaagat
     7681 tatcaattcc ttttgtatat ctcttatttc agtggtctct cccgcatttg cattcggacg
     7741 gaatgacagt cgtcattcgt cctctccagc cgcctcacac caattaacaa acacgtatat
     7801 acgaacacgg agtctccgtt atgaaaaaaa actatctgac acttatactg ccgtcattat
     7861 tgtccggctg tgccggaatt tccggcagca ataacgtggc accatcagat acgcctgctc
     7921 tggtttttgt tgacggacaa atcagtgaga gtgctggcgt tctggcactt acgcaacaac
     7981 gtctttctcc acctaaaata actccccccc cgagagtcag cgtgccagag acagtaaaaa
     8041 cagcacatcc agtcagcgaa tcccgaccgt taccttcacc aacaataagt cagaatttct
     8101 cccggttaat cattacaggc aagcctggta gcgcccctgt aacactatcg ccgacggccc
     8161 gtaaccggac cgtcagtcag tggataaaaa cattactacc ggaaggatgg aaattcagac
     8221 aagagaatgc agccacgccc aagctgaata ccaaactggt ttcatggtct gccaatgatc
     8281 agtggacccg ttctctgaat cggttgctgg aagaacaaca cctatggggc catctggact
     8341 gggataataa aacgttgacc gtcacaacat cagctgctgc accggcacag gacacggcaa
     8401 ccactatctc gccagcagcc atggtaacca catccacttc ccctgaatct ccaacaacag
     8461 caaatagcca gaacaaacca cgaaatccat tcagaggaaa cagcgtatct tcgtcaaccc
     8521 ccgccgccac cccgactcca accggctcca cagtcaagtc tatcccgctc atgaccggaa
     8581 cgcctgttaa accggtatct cagggtaaag agtggcgtgc gccggcaggc accacattaa
     8641 gagagaacat tatcaagtgg gcagaagaaa caaaatgcga aagcatggca tcaacgcact
     8701 ggatggtcat atggccactt tctgtaaccg attaccgtct ggatgcgcct ttaatgttcc
     8761 ggggatcatt tgaatcagcc atggagcagg tatttgagtt atatcggact gcgcaaaaac
     8821 ctttatacgc acaggcttct cgcatgcagt gcatcattac agtttctgat acaagggatg
     8881 gtcgctaatg ggatggctgg tcatggccgt cggcctgacc attctgatta tcactggtag
     8941 ctaccagaat cagaagatgt ctgaaaccac aaacgcccag caatatgcaa gtgcgtctgt
     9001 atgggcaagt cagatactga tgattgctaa cagaatcaat gatatcagat acgtatcagg
     9061 ccagcaggac ggagtaattt cctcagataa actggctctc cctgtcactc cggactcccg
     9121 aattaaacat cagttacaac aggggcgtct gtgggtatgg atgcctgaac aaccaggtct
     9181 tgttgaaact ttgcgttcaa aaagtcgtgg ttctgcacta atagggatct tccaaaacgg
     9241 gcaactcacc tggctatcag gaactgcaac cggtctgaca cccccggcag gcattactgc
     9301 tggttctgtc gtctacgtta actaatctgg atatattcat gaaaaaatca caccagcgtt
     9361 caatgaagct ggcggtgctc ccctgcatga tcgccgtcgc tctttccatc tccggatgta
     9421 ctttcagcga aatcaacaaa atgcagaaaa aagcacagga agactcagca catgcacggg
     9481 aaaaggtatc agccctttcg gcccgtaaat cgcaggctct tacctggctc gataatcaat
     9541 ggataaaccc tgttccggtc gctcaggtat caagagagaa aaaacaaaca gctccggcct
     9601 gctacatcac gcaggcaaga aaaggagaga tcactctgca ggaactgggg caacgtatta
     9661 ctgccgtatg cggcatccct gtgatcatca cgcctgacgc agccaattca actcttgaag
     9721 gaggcgctac ccgccagatg acaggaacac taccagcacc agatgaaaat gggcgtctac
     9781 cgttaagcag cctgggcagc acaacaatga ctacctccac tcagccatta acgctgaata
     9841 acctcatgtg gcagggagat atcaatggtc ttctggatct gatggccagc cggagtggtc
     9901 tgtactggcg catggataat ggtcggattg tattctatct gactgaaacc agaacgtatc
     9961 cacttcatat gctgaacacc aaaaccagca gcagttccag tgtcagctct ggctcaacaa
    10021 gcacaatggg ggcaacagga ggccaggata actcagcatc cggtgatgca acgtcctctc
    10081 agagcacaac cgttggtcag gaatacgatc tgtatgaaga catccggaaa actattgaag
    10141 caatgctgac accagaaaaa ggccgttact ggttatctgc atcgagctca acgctgactg
    10201 tcactgatac tccagctgtc caggaagccg tcgcacgata tgtggacgaa caaaacagta
    10261 ttatgaaccg ccaggtagcc ctgaacgtac aggttctgag cgtcagcaat accagaaacg
    10321 aacagttcgg tctggactgg aaccttgttt ataaatcgct acattccgcc ggagcaacgt
    10381 tgaacaatgc aagcggagat tttacaggcg ctacatctgc aggcgtatca attctggata
    10441 cggcaacagg gaatgccgcc aaattcagcg gttccagtct tctgattaaa gcgctgagtg
    10501 aacagggcga tgtcagtgtt gtgacttcac aagaaagcac tgtcacaaac ctgacgccgg
    10561 tacctatcca gatggcagat cagacggttt acgtcgccca gtcagcaaca acaacgacta
    10621 cggatgtagg agcaacaaca acattaacgc cgggcatgat caccaccgga ttcaatatga
    10681 ccctgctgcc tttaattcag aaaacgggca atctccagtt gcagatgaat tttaatctgt
    10741 cagatccccc aacaatccgt agctttacgt caaaagacgg aaacagttac atcgaaatgc
    10801 cgtataccaa actgcgttca ctgagccaga aggtcaatct gaaagaaggg caatcacttg
    10861 tcgttactgg tttcgatcag aacaatacga cgacaagtaa agccggtacg tttacgccag
    10921 caaatccatt atttggtggt tcacaaaccg ggaaaaatga acgcagcacg cttgtaatca
    10981 tcattacccc gactttcccg tcaggaggca acaatggctg atgaagatat caacccggtt
    11041 gtacttctcg cagatcctaa agtaaatcat cgcgtgtggg cagcctgtct taaatggagt
    11101 cctgtcgtaa aaaaacagcg agtcccttct catcagaaac acaagcccca tgtaaaatcc
    11161 agacggctta cctctctgaa agtgactgta ggcagcagaa gttcccgtgg gaagatcagc
    11221 cgcattaccg gcacaggaat actggccagg ccggagcgca atcattattt ttcccttgct
    11281 ctggctttct gctcatgggt gagaaatgga tacggcgtct tccggtacag cgataaggag
    11341 cttttattcc ttgcctccat taatggccag cctgccgtta tggctgatct gtcaggaaat
    11401 gatgctgatg ttgctcaaaa ggtcagtctt tttctgacga tgaacgaaga gccccccgaa
    11461 aaatggcagg tcgtatctcc tctggaacat cctgataact gggaaagtat tattacccgg
    11521 ttatcatcag cagatctccg ccgttgcaaa cttactgtcg gcaatcgcag caaattcaca
    11581 ctgcctgccg tacttttttt agtagcagcc agcgcaggca ctgtattctg gatgactcag
    11641 ccagagccgg atgtcgtacc gacgcccgag gagatagctg cacgggcaag attgcagttt
    11701 aaaaagcccg atccaccgcc agaactcccg catccatggg catcacaacc tgtaatcagt
    11761 gattttctga aagcctgcgc ggaccttcga aaaccatcac cggtcgcgct agaaggctgg
    11821 aaacttaccg gtggaacatg tactcccgag acattcacac tgatttacga acgacagcca
    11881 ggaggaacta ttgaaggctt tctggcaaga agcaaagaaa tctttaatgt tattcctgac
    11941 tttaacctta aagatggtgc acgcctggct tctgtaaccc ggccactgcc ttccctgccc
    12001 cgcagggatg aggcagtccc taccccctca gaacaactga tgcgggtgtt cacgtggttt
    12061 cagaaaaaac agttaacgcc ggccattaat gaaatagcca taccggaacc attaccaggt
    12121 aacgatggtg aaccagcccc tgttcagaaa tggaaagaat atcagttcag tttatcaacg
    12181 cctgtaaatc ctgatgagtt gttcccttta tttcaggata ccggcgttcg tctgagcaat
    12241 atccattttg aattaaacgg cggtactttc agttattcct cagaaggtca tatttatgcg
    12301 tccaggtaaa ttactcatta ttccatccat atttttcttc tccggttttt catttgcaac
    12361 aacacaacct cttgtcacaa taggagaact tgaagcacaa caaaaccgga atattctttt
    12421 acaggcaaaa gttcagggcg cacagctaca gaaacaactc gaagaatcag atgttacttc
    12481 atcatctgaa actgtttcag gtttctctgg tgtgactgcc tctttgccat cagtttcaga
    12541 gcagccgaca tctaaaaaca gaaaggaatt gcccgttatt atggaaataa acggcaaaga
    12601 taaacgtctt aatgccgttc ttcgcatggc tgatggacgt cagacaagcg tcaccactgg
    12661 cagccagctt ccaggcacat ccgtcacagt gaagtcgatc tcattgtctg gcgtcacgtt
    12721 aagtgatggc acccccctta ctttctgagt aacgtgatat ggataaatat aatctgaagg
    12781 atgctctcct ttttatatcc aggggagaca cccatgaaat tcttatagaa acaaaccagc
    12841 ggacaaggcc tgacgtacaa agtaatctcc aggagctgct aaaattatat ccggatatca
    12901 acccaaaagt agtctcgttg tcggagttac aggaggcagg tcaggatgag aaaaacagag
    12961 gcccgaaaga acgaattatt catctgaaag accttgctga tgtcagtgaa agtgagaaga
    13021 aagttctatc ttattttgaa actgccagaa aacttggtgc ttcagatatt catttcttaa
    13081 tttcagagtc tattttcaaa gttcgcatga gaatattcgg tgaactgcag actgtagatg
    13141 aagatcaacc agcactgggc tattcacttt gcgcaacagc cattctgtca atggctgatg
    13201 tcactgaaac cagtttcttc cctcaaaggg agcaggatgc gcgcctgtcc ccacaattaa
    13261 tgcgaaaaat aggaatattt ggtgcacgct acagtcacag acctacggga gacggtctga
    13321 ttgcagttat gcgcctgata cccgatgacg gagataaagt tcccacgttt aaacaactgg
    13381 gctttatccc ggaacaaatc cggttgctga acataatgct gcgccgcccg gaaggaaaaa
    13441 tagtcctgtc aggtccaacc ggttccggta aatcgacaac gttacgcagc gcctgccgtg
    13501 tatatcttga cgataatcag gggcgacacc tgctaacaat cgaagatccg ctggaaggac
    13561 agatactggg agcaacacag actccgatta tctgcgacaa atccgacgaa gatgccgtca
    13621 aactggcatg gagtcgggct atttcatcgg caatgcgact tgaccctgat gccatcatgg
    13681 aaggggaaat gcgcgatctt atctcgatga tgtcaaccac ctacgcggca cagaccgggc
    13741 acatagttct gacgacatta catacaaact cagctctggg tataccggag cgaatgatta
    13801 cgatgggtat gaatgccgat cttatttgcg atgcgcaatt actgattggg atgatcagcc
    13861 agcgccttgt accaactctg tgtccctcct gccgtattcc atgggaaaag cgagcacctg
    13921 aacttagcga cgacgagcgt gattaccttg agcggcactg caataaagat tcactctgca
    13981 gtacagataa tatctggttc cgaaatcatc acggatgctc tgaatgtaat cacgacgtaa
    14041 tcattaacgg cagaaagcgc ggtgaaatag gtaaaggcct caccggcagg actgtaattg
    14101 ctgaggtgat agaacctgat aaccgcttat ttcagattct gaaaacacgc ggcaaagttg
    14161 cagcacgtaa atactggctg gaaaacatga aaggcatcag tcgtgtagag catcttcttc
    14221 gccgtatcaa tgaaggtctg gtagaccctc ttgaagccga cagaatcatc cctctggacg
    14281 aagacgagcg cctcagcatc gatgatgtct aacaggagaa caacatgcgt gaaatgaatt
    14341 tcagtcaacg gctgaggcgt ttcattgtca gaaaaacgtt cagcgccccc tacagggtgc
    14401 agttttacga agccctgcgt tttctgctgg aaaataaaca gccattaaag accgcactgg
    14461 aacagatgcg ggatgcctgg acagactttg gacgaaaatg gcatcccttt gctgaactgg
    14521 ccacagactg cattgagtct cttcgtgaaa acagtggcga aaactctctg gaatatactc
    14581 ttagtttatg ggttcctcag gaagaggccg cagtaatcag cgccgggata agaagtggtt
    14641 caattgtgga tgcactgcaa ttcgccacca ctcttacaga tgcaaaagaa caaatccatc
    14701 aggctatatg gcaaatggct atttatccgg tgggattgtt aatcatgatg acggggacat
    14761 tatatgttct gaatacagag ctaatacctg aactgagcaa aataagctcc cccgattcat
    14821 ggagtggtgc tcttggtttt ctttatggat tatctgtttt tgtcgataat tacggcgcta
    14881 tatgtgccgt tctttttgca gtgatcacag gtttaatatc atggtctctg ccaaactgga
    14941 aaagccctga cagtgttcgt acttttgccg acaagataat gccatggagt atttatcagg
    15001 atatacaggg agcaacattt ctcctgaata tggctgcact tctgaaagcc aaaatgacga
    15061 cgctcaactc gctaaacatt cttcaggagt ttgcatcacc atggttaagc acacgtcttg
    15121 acagcattat ttaccgcgtc cgccaggggg atcatcttgg actggctttg cgtcagtgcg
    15181 gttatcagtt cccatccagg gaagcagcca acttcctgtc tttattacag ggagatggcg
    15241 caacagagct aatcagcaat tatggtcaac gatggcttag tcaaacactt caacgtgtaa
    15301 agaaacgggc aaacgtgatt cgtttaatta tgctgatatt tcttgtcatg tctctcatgt
    15361 tactggtttt tgccattatg gatattcagt ctattagtga caacagcatg ggtaactttt
    15421 aaacaaaacg aggatattta tgttagtaga aaatattaat accactttaa ctggaaataa
    15481 taagaagaat gagcctcatg ataaaggatg ggcaattttg gagcagggaa caatagcgct
    15541 cgttgttctt tttgttatag tcgttgttct gggtagtttg tatgcgttgc gtacaagagc
    15601 caatgtggca actgaaaccg caaatataca aacaattatc acaagtgccc agagcctgct
    15661 aaaaggtagc gatggatata catttaccag cagtgccaaa atgacaggtg ccctcattca
    15721 gatgggtgtc attccctctg ggatgaccgt acagggcgac aaaacatctg gtacagcaac
    15781 gctctataac gcatggggcg gtgctgtaac tgttgccccg gcttcaacat cagggttcaa
    15841 caacggtttt accgttacct atgataaagt cccacaggat gcctgtatcc agattgccac
    15901 acgaatcagt aaaactggtt taactaacgg cattaccctg aacagtactg ctcacagtga
    15961 tggaaaagtc actactgaag aagccagtac gcaatgcaag gcagataatg gcagcactgg
    16021 tacaaacaaa cttatcttca ccatcaatgg ttaaggagtt ttaatgtcag ggggtacagt
    16081 acccctcaga cttatttcat taatcctgct tttttctctt attccacccg cccgagcttc
    16141 atacgtagct cccaaacaga tctggaccaa caagtgggac aattgctttg ccgctgccgg
    16201 cgcgcgctat caaattgaac cattattatt gaaggctatt tctgccgggg aatcttcgtt
    16261 gaggccgggc gcaattaata taaataagga caaaaaaaca ggtaaagcca gtagcacgga
    16321 ttatgggttg atgcaaataa actcaaccca tattccaaag ctgataaata tgggcgtaat
    16381 aaaaaagtct gaggatttaa ttaccaaacc atgccttaat atccatatag gaagctggat
    16441 actggcaaga cattttcaga tatgtggcgt cagctggaat tgccttgggt catacaatgc
    16501 cggattccgt aaggacagac atgaaacaag agagcaatat gccaataaaa tatggagaat
    16561 ttatcgtgac atgaaaggga tatgtcttcc cggacaggga ggcaggcaat gcagacaatc
    16621 gtaggcataa gtctatcccc tgtttatata ctgccattga tgttcacatt ttccattatt
    16681 ggacgaactc tgctattcag agttcgttat tttttatctg atacgggaca tctctggtat
    16741 aagacacacc ctgctgttct gtcaggaata tggctataca gtatagctgt ggcgttaatt
    16801 attttatcat catcaccact gctctaccgt attcatgctg tccttattct ttcgttcata
    16861 ctgcaaatgg cggtaacgga tgcactgaca ggtttattac caggaacatt cacccgacgt
    16921 tttttaattg caggaatgct atcacaaatc acgactgata tatggtggtt ccgtacaaca
    16981 gaatttgcca ctgctgcaat tgtcttattc tgccttcata aattagttaa ccgccatcgt
    17041 cttaatattg gcactggaga cctgtggctt attgcaggta ttaccgcatg gtcagggtta
    17101 tataacgcca tatggtgtgt gttgctgggc acaggaggat ttgtcttatg gcattcaaca
    17161 tggtgcataa aagggcataa agaaggccct ctgggtccat ggctatgttt tggccatgtc
    17221 cttttgctgc tggataatct ttatcaacca ctatgggtaa tctgatgaaa aaatatgacc
    17281 gaggctgggc atcgctggaa actggcgcgg ctttactgat tgttatgctc ctgattgcct
    17341 ggggagcggg catatggcag gactatattc aaacaaaagg atggcaaact gaagcacgtc
    17401 ttgtcagcaa ctggaccagt gcggcccgct cttatatagg gaaaaactac acgactctcc
    17461 agggcagtag taccacaaca actcctgccg ttatcacgac aaccatgctg aaaaataccg
    17521 gctttttgtc cagcgggttt actgagacaa acagcgaggg gcagcggtta caggcatatg
    17581 tggttcgaaa cgcccaaaac ccggaattac tacaggcaat ggttgtatcc agtggtggca
    17641 caccttatcc agtgaaagca cttatccaga tggctaagga tattaccact ggtcttggtg
    17701 gatatatcca ggacggcaaa acagccacag gtgcattacg ttcctggtca gtagctttaa
    17761 gtaattatgg tgccaaaagc ggtaacgggc atattgccgt attgttatcg acagatgaac
    17821 ttagtggtgc agctgaggac actgatcgtc tttacagatt ccaggtcaat ggtcgccctg
    17881 acttaaacaa aatgcacacg gccattgata tgggatcaaa taacctgaat aacgttgggg
    17941 cagtaaatgc ccagacaggt aatttcagcg gcaatgtgaa tggtgtaaat ggcactttca
    18001 gcggtcaggt aaaaggcaat agcggaaact ttgacgtaaa tgtcaccgct ggcggtgata
    18061 tcagaagtaa taatggttgg ttaattactc gtaacagtaa aggctggctc aatgaaactc
    18121 acggtggcgg attttatatg tccgatggat catgggttcg aagtgtaaac aacaagggca
    18181 tctataccgg cggtcaggtg aaaggcggta ctgttcgggc tgatggtcgc ctttatactg
    18241 gtgaatactt acaactggaa agaactgccg ttgctggcgc atcatgttcg cctaacggcc
    18301 ttgtaggccg cgataataca ggggcaat
//
LOCUS       DAMZET010000083        17928 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.083, whole genome shotgun sequence.
ACCESSION   DAMZET010000083 DAMZET010000000
VERSION     DAMZET010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.083"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            91..1233
                     /locus_tag="POB34_002853"
     CDS             91..1233
                     /locus_tag="POB34_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI5919763.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHCERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1240..2004)
                     /gene="glcC"
                     /locus_tag="POB34_002854"
     CDS             complement(1240..2004)
                     /gene="glcC"
                     /locus_tag="POB34_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HDI5919764.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2255..3754
                     /gene="glcD"
                     /locus_tag="POB34_002855"
     CDS             2255..3754
                     /gene="glcD"
                     /locus_tag="POB34_002855"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HDI5919765.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3754..4806
                     /gene="glcE"
                     /locus_tag="POB34_002856"
     CDS             3754..4806
                     /gene="glcE"
                     /locus_tag="POB34_002856"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HDI5919766.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGEGGFAPLPAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4817..6040
                     /gene="glcF"
                     /locus_tag="POB34_002857"
     CDS             4817..6040
                     /gene="glcF"
                     /locus_tag="POB34_002857"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HDI5919767.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERILREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAVRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6045..6449
                     /locus_tag="POB34_002858"
     CDS             6045..6449
                     /locus_tag="POB34_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HDI5919768.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6471..8642
                     /gene="glcB"
                     /locus_tag="POB34_002859"
     CDS             6471..8642
                     /gene="glcB"
                     /locus_tag="POB34_002859"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HDI5919769.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAVVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAALTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNADDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8998..9381
                     /locus_tag="POB34_002860"
                     /pseudo
     CDS             8998..9381
                     /locus_tag="POB34_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
     gene            9381..10370
                     /locus_tag="POB34_002861"
                     /pseudo
     CDS             9381..10370
                     /locus_tag="POB34_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspD"
     gene            10370..11863
                     /gene="gspE"
                     /locus_tag="POB34_002862"
     CDS             10370..11863
                     /gene="gspE"
                     /locus_tag="POB34_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HDI5919770.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            11863..13085
                     /gene="gspF"
                     /locus_tag="POB34_002863"
                     /pseudo
     CDS             11863..13085
                     /gene="gspF"
                     /locus_tag="POB34_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
     gene            13102..13557
                     /gene="gspG"
                     /locus_tag="POB34_002864"
     CDS             13102..13557
                     /gene="gspG"
                     /locus_tag="POB34_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HDI5919771.1"
                     /translation="MYSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            13561..14124
                     /gene="gspH"
                     /locus_tag="POB34_002865"
     CDS             13561..14124
                     /gene="gspH"
                     /locus_tag="POB34_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HDI5919772.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASSGVVQTFATDSEPPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDAPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERMP"
     gene            14121..14492
                     /gene="gspI"
                     /locus_tag="POB34_002866"
     CDS             14121..14492
                     /gene="gspI"
                     /locus_tag="POB34_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HDI5919773.1"
                     /translation="MKRGFTLLEVMLALAFFALAATAVLQIASGALSNQQILEEKTVA
                     GWVAENQTALLYLMTREQRAVRQQGESDMAGSRWYWRTTPLSTGNALLQAVDIEVSLH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            14489..15088
                     /gene="gspJ"
                     /locus_tag="POB34_002867"
     CDS             14489..15088
                     /gene="gspJ"
                     /locus_tag="POB34_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HDI5919774.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQ"
     gene            15091..16068
                     /gene="gspK"
                     /locus_tag="POB34_002868"
     CDS             15091..16068
                     /gene="gspK"
                     /locus_tag="POB34_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HDI5919775.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALITRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            16065..17243
                     /gene="gspL"
                     /locus_tag="POB34_002869"
     CDS             16065..17243
                     /gene="gspL"
                     /locus_tag="POB34_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HDI5919776.1"
                     /translation="MSSILEIFFPLCAADPIHWQRRTPDVEHGIWSDVANEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPVVARKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGLALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAVTTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALAADFVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            17245..17781
                     /gene="yghD"
                     /locus_tag="POB34_002870"
     CDS             17245..17781
                     /gene="yghD"
                     /locus_tag="POB34_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HDI5919777.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETILQQLVAMNTRLKSAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIRRIAERGENIQVWIEPVVFNDLLKWLNALDEKYALRVTQIDVS
                     AAEKPGMVNVQRLEFGRG"
BASE COUNT         4127 a         4611 c         5213 g         3977 t
ORIGIN      
        1 tgtttgtatt cattgggttg attattaagg tgtcgttcgt tgctgttgca acctgttttt
       61 caccgttaat tatttgttat ccacagagat atggaatgcg atctgctgat gattaaaatt
      121 gaaaaagtaa ttaataaaaa tgaccttaag gcatttatcg cttttccgtc atcactttat
      181 cccgacgatc caaactggat acccccttta ttcattgaac gcaacgagca tttgtctgcg
      241 aaaaatccgg ggacggacca tatcatctgg caggcgtggg tggcaaaaaa agcggggcag
      301 atagttgggc gtattaccgc gcaaatagat accttgcact gtgagcgtta cggtgaagat
      361 accggtcatt tcggcatgat tgacgccatt gatgatccgc aggtttttgc cgcgttgttt
      421 ggcgcagcgg aagcgtggtt gaagtcacaa ggtgcaagta agatcagcgg gcctttcagc
      481 ctgaatatca atcaggaaag cggattactg attgaaggtt ttgacacacc accctgtgcg
      541 atgatgccac acggcaaacc gtggtatgcc gcgcatattg aacaactggg ttatcacaaa
      601 ggcattgatt tactggcgtg gtggatgcag cgaaccgatc tcactttctc tccggcgcta
      661 aaaaaactga tggatcaggt gcgcaaaaag gtgaccattc gctgcatcaa tcgtcagcgg
      721 tttgccgaag agatgcagat cctgcgtgag attttcaact ctggctggca gcacaactgg
      781 ggatttgtgc cgtttaccga acatgaattc gcgaccatgg gcgatcaact taaatatctg
      841 gtgccggacg atatgatcta tatcgctgag attgattctg caccctgcgc gtttattgtc
      901 ggcttgccga acatcaacga ggcgattgcc gatctgaacg gatcgctctt tcccttcggc
      961 tgggcaaaat tgctgtggcg tttgaaagtc agcggtgtgc gaactgcgcg agtaccgctg
     1021 atgggcgtgc gcgacgagta tcagttcagc cgcatcggcc cggtgattgc gctgttattg
     1081 attgaagcct tacgcgatcc gtttgcccgc cggaagatcg atgcgctgga gatgtcatgg
     1141 atcctcgaaa ccaatacagg catgaataac atgctggaac gcattggggc ggagccgtac
     1201 aagcgttacc gattgtatga aaagcagatt taataccagc taactcaggt tcatctccag
     1261 cggcaccgcc gagcgaatca aatggtggcc ttccagctcg atatcgtgga gattcttttt
     1321 cacggtccgc acatgatcgc gtgctgcgcg ctgggcgacg tgcggcagcc gctgcaacac
     1381 cgcgttgtag atcctcgcat gctggcgatc gatctgcttt ttttgctgtg gtcgatggta
     1441 gagattattt acgctggcaa acactgaatt aaacatcaga tcggtcaatg attgcagcgt
     1501 aaacaccagc acctgattgt gagaagcctg acaaatggcg agatggaaag cgtgatccaa
     1561 ctgcgcatgt tcgatcagcg aaatctcttt gttgttctca ctggcggcga gcattttttc
     1621 ataacagcgg gttatcacaa caaaatcagc ctgcgttccc agcgttgccg ccagccttgc
     1681 cgattcgccc tccagtaatg cgcgaacgtc gagcagatcg tacagcgttc gcggctgcgt
     1741 actgaacaga tggatcagcg ggctggtgtc ctgcacccga ttaagccgtg cgacacgaga
     1801 atcgcgaccc tgcgccgtct caataatccc gcgcccgcgc agcacggtca gcccttcacg
     1861 cagtgcggag cgtgagaagc cgagcttttc acacagtcga cgttccgagg gaagtggctg
     1921 accgaccttc agtacgccgt cgataattaa ccgttcgata ctctctgcaa ccacttcgca
     1981 aatagggcga cgttcatctt tcattcccgg acctcgtgca caggtaggac caatttttct
     2041 gttcttaact cgcaaaacac gcacatcacg taagtgatta tgttacatca atttaacatt
     2101 gagttaacca aggcaaggtc acagagctgg aaaaaaaatg gtctgaccgg tagctaaaga
     2161 gatagacgaa aacgaaaagc ccgcttaata actgttcaca gaagcagcgc gcaaaaatca
     2221 gctgccacac aacacaacaa agcgaagcct actcatgagc atcttgtacg aagagcgtct
     2281 tgatggcgct ttacccgatg tcgaccgcac atcggtactg atggcactgc gtgagcatgt
     2341 ccctggactt gagatcctgc ataccgatga ggagatcatt ccttacgagt gtgacgggtt
     2401 gagcgcgtat cgcacgcgtc cattactggt tgttctgcct aagcaaatgg aacaggtgac
     2461 agcgattctt gctgtctgcc atcgcctgcg tgtaccggtg gtgacccgtg gtgcaggcac
     2521 cgggctttct ggtggcgcgc tgccgctgga aaaaggtgtg ttgttggtga tggcgcgctt
     2581 taaagagatc ctcgacatta accccgttgg tcgccgcgcg cgcgtgcagc caggcgtgcg
     2641 taacctggcg atctcccagg ccgttgcacc gcataatctc tactacgcac cggacccttc
     2701 ctcacaaatc gcctgttcca ttggcggcaa cgtggcagaa aatgccggcg gcgtccactg
     2761 cctgaaatat ggtctgaccg tacataacct gctgaaaatt gaagtgcaaa cgctggacgg
     2821 cgaggcgctg acgctgggat cggacgcgct ggattcacct ggttttgacc tgctggcgct
     2881 gttcaccgga tcggaaggta tgctcggcgt gaccaccgaa gtgacggtaa aactgctgcc
     2941 gaagccgccc gtggcgcggg ttctgttagc cagctttgac tcggtagaaa aagccggact
     3001 tgcagttggt gacatcatcg ccaatggcat tatccccggc gggctggaga tgatggataa
     3061 cctgtcgatc cgcgcggcgg aagattttat tcatgccggt tatcccgtcg acgccgaagc
     3121 aattttgtta tgcgagctgg acggcgtgga gtctgacgta caggaagact gcgagcgggt
     3181 taacgacatc ttgttgaaag cgggcgcgac tgacgtccgt ctggcacagg acgaagcaga
     3241 gcgcgtacgt ttctgggccg gtcgcaaaaa tgcgttcccg gcggtaggac gtatctcccc
     3301 ggattactac tgcatggatg gcaccatccc gcgtcgcgcc ctgcctggcg tactggaagg
     3361 cattgcccgt ttatcgcagc aatatgattt acgtgttgcc aacgtctttc atgccggaga
     3421 tggcaacatg cacccgttaa tccttttcga tgccaacgaa cccggtgaat ttgcccgcgc
     3481 ggaagagctg ggtgggaaga tcctcgaact ctgcgttgaa gttggcggca gcatcagtgg
     3541 ggaacatggc atcgggcgag aaaaaatcaa tcaaatgtgc gcccagttca acagcgatga
     3601 aatcacgacc ttccatgcgg tcaaggcggc gtttgacccc gatggtttgc tgaaccctgg
     3661 gaaaaacatt cccacgctac accgctgtgc tgaatttggt gccatgcatg tgcatcacgg
     3721 tcatttacct ttccctgaac tggagcgttt ctgatgctac gcgagtgtga ttacagccag
     3781 gcgctgctgg agcaggtgaa tcaggcaatt agcgataaaa cgccgctggt gattcagggc
     3841 agcaatagca aagccttttt aggtcgccct gtcaccgggc aaacgctgga tgtgcgttgt
     3901 catcgcggca ttgttaatta cgacccgacc gagctggtga taaccgcgcg tgccggaacg
     3961 ccgctggtgg cgattgaagc ggcgctggag agcgccgggc aaatgctccc ctgcgagccg
     4021 ccgcattatg gtgaagaagc cacctggggc gggatggtcg cctgcgggct ggcggggccg
     4081 cgtcgcccgt ggagcggttc ggtccgcgat tttgtcctcg gcacgcgcat cattaccggc
     4141 gctggaaaac atctgcgttt tggtggcgaa gtgatgaaaa acgttgccgg atacgatctc
     4201 tcacggttaa tggccggaag ctacggttgt cttggcgtgc tcactgaaat ctcgatgaaa
     4261 gtgttaccgc gaccgcgcgc ctccctgagc ctgcgtcggg aaatcagcct gcaagaagcc
     4321 atgaacgaaa tcgcccagtg gcaactccag ccgctaccca ttagtggctt atgttacttc
     4381 gacaatgcgt tgtggatccg ccttgagggc ggcgaaggat cggtaaaagc ggcgcgtgaa
     4441 ctgctggggg gcgaagaggt tgcgggtcag ttctggcagc aattgcgtga acaacaactg
     4501 ccgttcttct cgttaccagg taccttatgg cgcatttcat tacccagtga tgcgccgatg
     4561 atggatttac ccggcgagca actgatcgac tggggcgggg cgttacgctg gctgaaatcg
     4621 acagccgatg acaatcaaat ccatcgcatc gcccgcaacg ctggcggtca tgcgacccgc
     4681 tttagtgccg gagagggtgg ctttgccccg ctaccggctc ctttattccg ctatcaccag
     4741 cagcttaaac agcagctcga cccttgcggc gtgtttaacc ccggtcgcat gtacgcggaa
     4801 ctttgaggag caggctatgc aaacccaatt aactgaagag atgcggcaga acgcgcgggc
     4861 gctggaagcc gacagcatcc tgcgcgcctg tgttcactgc ggattttgta ccgcaacctg
     4921 cccaacctat cagcttctgg gcgatgaact ggacgggccg cgcgggcgca tctatctgat
     4981 taaacaggtg ctggaaggca acgaagtcac gcttaaaaca caggagcatc tcgatcgctg
     5041 cctcacttgc cgcaattgtg aaaccacctg tccttctggt gtgcgctatc acaatttgct
     5101 ggatatcggg cgtgatattg tcgagcagaa agtaaaacgc ccactgccgg agcgaatact
     5161 gcgcgaagga ttgcgccagg tagtgccgcg tccggcggtc ttccgtgcgc tgacgcaggt
     5221 agggctggtg ctgcgaccgt ttttaccgga acaggtcaga gcaaaactgc ctgctgaaac
     5281 ggtgaaagct aaaccgcgtc cgccgctgcg ccataagcgt cgggttttaa tgctggaagg
     5341 ctgcgcccag cctacgcttt cgcccaacac caacgcggca actgcgcgag tgctggatcg
     5401 tctggggatc agcgtcatgt cagctaacga agcaggctgt tgtggcgcgg tggactatca
     5461 tcttaatgcg caggagaaag ggctggcgcg ggcgcgcaat aatattgatg cctggtggcc
     5521 cgcgattgaa gcaggtgctg aggcaatttt gcaaaccgcc agcggttgcg gcgcgtttgt
     5581 caaagagtac gggcagatgc tgaaaaacga tgcgttatat gccgataaag cgcgtcaagt
     5641 cagtgaactg gcggtcgatt tagtcgagct actgcgcgag gaaccgctgg aaaaactggc
     5701 agttcgcggc gataaaaaac tggccttcca ctgcccgtgt accctacaac atgcgcaaaa
     5761 gctgaacggc gaagtggaaa aagtgttgct tcgtcttgga tttaccttaa cggacgttcc
     5821 cgacagccat ctgtgctgcg gttcagcggg aacatatgcg ttaacgcatc ccgatctggc
     5881 acgccagctg cgggataaca aaatgaatgc gctggaaagc ggcaaaccgg aaatgatcgt
     5941 caccgccaac attggttgcc agacgcatct ggcgagcgcc ggtcgtacct ctgtgcgtca
     6001 ctggattgaa attgtagaac aagcccttga aaaggaataa aaaaatgaaa actaaagtca
     6061 ttcttagcca gcaaatggcg agtgcaatta ttgccgcagg tcaggaagag gcgcagaaaa
     6121 ataactggtc tgtttccatt gctgttgccg atgacggcgg tcatctgctg gcgttaagtc
     6181 gcatggacga ttgcgcgccg attgcggctt acatctccca ggagaaagcg cgtaccgccg
     6241 cgctggggcg tcgtgaaact aagggctatg aagagatggt gaacaacgga cgtaccgcgt
     6301 tcgtgactgc gccgttactg acgtcgctgg aaggtggcgt accggttgtt gttgatgggc
     6361 aaattattgg tgccgtgggc gtttctggtt taaccggagc acaggatgcg caggtcgcga
     6421 aagcggcagc agcggtgttg gcgaaataag cgaaaacgag gagataaaca atgagtcaaa
     6481 ccataaccca gggccgttta cgcattgacg ccaattttaa acgttttgtg gatgaagaag
     6541 ttttaccggg aacagggctg gacgctgcgg cgttctggcg caattttgat gaaatcgttc
     6601 acgatctggc accagaaaat cgtcagttgc tggcagaacg cgatcgcatt caggcggcgc
     6661 ttgatgagtg gcatcgcagc aatccggggc cggtaaaaga taaagcggcc tataaatctt
     6721 tcctgcgtga actgggctac ctggtgccgc aaccggagcg cgtgacggtg gaaaccacgg
     6781 gcattgacag cgaaatcacc agtcaggcgg ggccacagct ggtggttccg gcaatgaacg
     6841 cccgctacgc gctgaacgcg gcgaacgctc gctggggctc actgtacgat gcgttatacg
     6901 gcagcgacat catcccgcag gaaggggcgg tggtcagcgg ctacgatccg caacgcggtg
     6961 agcaggttat cgcctgggtt cggcgtttcc tcgatgaatc tctaccgctg gaaaacggca
     7021 gctatcagga tgtggtggcg tttaaggtgg tcgataaaca attacgcatc cagttgaaaa
     7081 atggtaaaga aaccacgtta cgtaccccgg cgcagtttgt cggttaccgt ggcgatgccg
     7141 ctgcgctgac ctgcattttg ctgaaaaata acggcctgca tattgaactg caaattgatg
     7201 ccaacgggcg gattggcaaa gacgatccgg cgcacatcaa cgatgttatc gtcgaagctg
     7261 ctatcagtac cattctcgac tgcgaagatt cggtcgcggc ggttgatgcg gaagataaaa
     7321 tcctgctgta ccgcaatctg ctgggcctga tgcaggggac tctgcaagag aaaatggaga
     7381 aaaacggtcg acaaatcgtg cgtaaactga atgacgatcg tcattacacc gccgccgatg
     7441 gctctgaaat ttctctgcac ggacgctcgc tgctgtttat ccgcaacgtg ggtcatttga
     7501 tgaccattcc tgtgatttgg gacagcgaag gcaatgaaat cccggaaggc attcttgatg
     7561 gcgtcatgac tggcgcgatc gccctctatg atttaaaagt gcagaaaaac tcgcgcactg
     7621 gcagcgtcta tattgtgaaa ccgaaaatgc acggtccgca ggaagtggcg ttcgccaaca
     7681 aactgtttac ccgcattgag acaatgctcg gtatggcacc gaataccctg aaaatgggca
     7741 ttatggatga agaacgtcgg acctcgctga acttgcgtag ctgtatcgct caggcgcgca
     7801 atcgcgtggc gttcatcaat accggtttcc tcgaccgtac cggcgatgaa atgcattcgg
     7861 tgatggaagc tggcccgatg ctgcgtaaaa accagatgaa atcgacgccg tggatcaaag
     7921 cctacgagcg taataacgtg ctttccggtc tgttctgtgg gctgcgcggt aaagcgcaaa
     7981 ttggtaaagg catgtgggca atgccggacc tgatggcaga catgtacagc cagaagggcg
     8041 accaactgcg tgccggggca aacacagcct gggttccgtc accaaccgct gctacgctcc
     8101 atgcgctgca ctaccaccaa accaacgtac agagcgtgca agccaacatt gcccagaccg
     8161 agttcaatgc tgaatttgaa ccgctgctgg acgatctgct gactattccg gtggctgaaa
     8221 acgctaactg gtcggtggaa gagatccaac aagagctgga taacaacgtg caggggattc
     8281 tggggtacgt ggtgcgctgg gtggagcagg ggattggttg ttcaaaagtt ccggatattc
     8341 acaatgtggc gctgatggaa gaccgcgcaa cgctgcgtat ctccagccag catatcgcca
     8401 actggttacg tcacggtatt ctgaccaaag aacaggtgca ggcgtcgctg gagaatatgg
     8461 cgaaagtggt tgatcagcaa aacgctgacg atccggctta tcgtccgatg gcggggaatt
     8521 tcgctaactc gtgtgctttt aaagctgcca gcgatttaat cttcctcggc gtgaaacagc
     8581 caaacggcta taccgaaccg ttattacacg cctggcgttt acgcgaaaaa gaaagtcatt
     8641 aatagcgtca aaaaaagccc cggcaagttt tattgtcggg gctatgatag taactgcggt
     8701 agtacattta tcccggaaat aaccggagcg attatcaggg tttaacaata ttaaataccc
     8761 gtagcgcatt actagcgggc ctataacaac aatatggaca tcttaacgat actttgcgcc
     8821 tgcgtgtgca gaacagaaac ccttacccga tatacttgtt atgccagagg tagcaattgc
     8881 gttagcggcg tactgttgct gtaaagtata acgcctatat tttacagttc agtcctcttt
     8941 tgcttattac aaccttaagt atatcaagca tataaagata ataagagact gaacaatatg
     9001 gttacctgga cccaaatgta tatgccgatg ggaggactgg ggctatccgc tctggtcgcc
     9061 ctgatcccga taatattctt cttcgttgca ctcgcggtat tacgtctgaa aggacatgtc
     9121 gctggagcaa taacccttat attatctatc ctgattgcaa tattcgcctt taaaatgccg
     9181 attgatatgg cttttgctgc tgcgggctat ggctttattt atggattatg gcctattgcg
     9241 tggattattg tcgcggcggt gttcctctat aaattaaccg ttgccagcgg tcagttcgat
     9301 attatccgca gctcggttat ctccatcacc gacgatcagc gtttgcaggt gttactgatt
     9361 ggtttctcct ttggtgcgtt attcgccgtg ctcaggtgca cgtcgaggcg ttgatcgtgg
     9421 aagttgccga aggtagcaat atcaacttcg gcgtgcagtg ggcgtcgaaa gatgccggat
     9481 taatgcagtt tgctaatggt acgcagatcc ctatcggtac gctgggggca gccatttctc
     9541 aggcgaaacc gcagaaaggc tcgacggtga tcagtgaaaa cggcgctacc accattaatc
     9601 cggataccaa cggcgatctc tccacgctcg cccagcttct ttccggcttt agcggtacgg
     9661 cggttggtgt ggtgaaaggc gactggatgg cgctggtgca ggcggttaaa aacgactcca
     9721 gctcgaacgt gctctccacg ccgagcatca ccacgctgga caaccaggaa gccttcttca
     9781 tggtgggcca ggacgttccg gtattaaccg gctctaccgt tggctccaat aacagcaatc
     9841 ctttcaatac agtagaaagg aaaaaagtcg gcatcatgct gaaagtcacg ccgcagatta
     9901 acgaaggtaa cgcggtacag atggtgattg agcaggaagt ctcgaaggtg gaaggacaga
     9961 ccagcctcga cgtggtgttt ggtgagcgca aactgaaaac caccgtgctg gctaacgatg
    10021 gtgagctgat tgtgcttggt ggtctgatgg acgatcaggc gggagaaagc gtggcgaaag
    10081 tgccgctgct gggcgatatc ccattgattg gtaacctgtt taaatcgacg gcggataaaa
    10141 aagaaaaacg taacctgatg gtgtttatcc gcccgaccat tctgcgtgac ggtatggcgg
    10201 cagacggcgt gtcgcagcgc aaatataact atatgcgcgc cgaacagatc tatcgtgatg
    10261 agcaaggttt aagcctgatg ccgcacaccg cacagccggt actgccagcg caaaaccagg
    10321 ccttaccgcc ggaagttcgc gcgttcctca atgccgggag aacgcgttaa tggtgcctgt
    10381 agcacaggaa accaccgcca acaccgtgcg tctgccctac agtttcagcc gtcggtttag
    10441 cctggtggca tggtgcgaag cgtcgctgga gatcctccac gttcatccgc tatcgctctc
    10501 tgttttgcag gagctgcagc gggggctgaa cgcgcccttt acgctgcggc aaatcgacga
    10561 ggccgaattt gaacagcggc tgaatgcggt ctggcagcgg gactcttccg aggctcgcca
    10621 gctgatggaa gatctcggtt ctgctgagga cttttttact ctcgcggaag aactgccaga
    10681 aacggaagat ctgctggaaa gcgacgacga tgcgccgatc atcaaactga tcaacgccat
    10741 gctggcagag gcaatcaaag aaggcgcttc ggatatccac attgagacgt ttgaaaagag
    10801 tctggtgatc cgttttcgtg ttgacggcac attacatgaa atgctgcgtc cggggcgcaa
    10861 actggcctcg ctgctggtgt cgcgtatcaa ggtgatggcg cggctggaca ttgccgaaaa
    10921 gcgcgtgccg caggatggac gtattgcgct gttgctgggc ggccgggcga ttgacgtgcg
    10981 tgtctccacc atgccttctg cctggggcga gcgcgtggtg ctgcgactgc tggacaaaaa
    11041 ccaggcccgc ctgacgctgg agcgtctggg gcttagccag caactgaccg cgcagttgcg
    11101 ccagctgtta cacaaaccgc acggcatctt tctggtgacg gggccgacgg gttccggcaa
    11161 aagcaccacg ctgtacgccg gattgcagga gctgaacaac cattcgcgca acattctcac
    11221 ggttgaagat cccatcgaat acatgattga agggatcggt cagacgcagg ttaacacccg
    11281 cgtcggcatg accttcgccc gtggcctgcg cgcgattttg cgtcaggacc cggatgtggt
    11341 gatggtcggt gaaatccgcg ataccgaaac cgcagaaatc gccgtccagg cttcacttac
    11401 cggacacctg gtcctttcca cgctgcatac caacacagcg gtgggggcga tcacacgttt
    11461 gcaggatatg ggcgtggagc ctttcctgct ctcttccagt ctgacgggcg tgatggcgca
    11521 gcgactggtt cgcacgctgt gtcccgactg ccgccagccc gcaccagcca ctgacgaaga
    11581 aaaacgcctg ctgggaatta ccgacgcccg taccgtcact ctgtaccatc cacagggctg
    11641 tcccgcctgt aatcacaaag gttttcgcgg acggactgcc atccatgagc tgatcgtggt
    11701 ggatgccaca ttgcgtgatt tgatccaccg tcaggccggg gagctggagc tggaacgtta
    11761 tgtccgacaa cactctgcgg gtatccgcag caacggcatt gagaaagtgc tcgccggaga
    11821 aacctctctc gatgaagttc tgcgggtaac catggaggcg taatggcact gttttactat
    11881 caggcgctgg agcgtaatgg tcgcaaaacc aaaggtatga ttgaggcgga ttccgcgcgt
    11941 catgcccgcc agttgttgcg cggtaaagag cttatccccg tgcatattga agcccggatg
    12001 aatacttcgt cagggggatg ttgcagcgtc ggcggcacgc acatcgtcgc gtggcggcgg
    12061 cagatctggc gctgttcacg cgccaactgg caacgttagt acaggcagca atgccgctgg
    12121 aaacctgctt acaggcggta agtgagcaaa gtgaaaaact gcatgtaaaa agcctcggaa
    12181 tggcgctgcg cagccggatt caggaaggtt acaccctgtc ggacagcctg cgcgaacatc
    12241 cccgcgtctt tgattccctg ttttgttcga tggttgctgc cggagaaaaa tccgggcatc
    12301 tcgacgtggt gctcaatcgc ctggcagatt acaccgaaca gcgacagcgc ctgaaatcac
    12361 gtctgttgca ggccatgctc tatccgctgg ttctgctggt ggtggcaacg ggcgtagtca
    12421 ctattttgct gacggcagtg gtgccgaaaa tcatcgaaca gtttgatcat ctcggacacg
    12481 cgctgcccgc ctccacccgc acgcttattg ccatgagcga cgcgttacag gccagcggcg
    12541 tttactggct ggcgggtttg ctggggcttc tggtgctggg gcaacggcta cttaaaaatc
    12601 ctgctatgcg cctgcgctgg gatcaaaccg tgctgcgcct gcctgtgacg gggcgtgttg
    12661 cgcgcgggct gaatacggcg cgtttttccc gcaccttaag catcctcacc gccagcagtg
    12721 ttccgctact ggaaggtatt cagaccgctg ccgccgtgtc ggcaaatcgc tatgtcgaac
    12781 aacaactact gctggcggca gatcgcgtcc gcgaaggaag cagtctgcgc gccgcgctgg
    12841 cggagttgcg cctgttcccg ccgatgatgc tgtacatgat cgcctccggc gaacagagcg
    12901 gcgaactgga aaccatgctt gagcaggccg cagtcaacca ggaacgggaa tttgataccc
    12961 aggtggggct ggcgttaggg ctgtttgaac cggcgctggt ggtgatgatg gcgggcgtgg
    13021 tgctgtttat cgtcatcgcc atccttgagc cgatgctgca actgaacaat atggttggaa
    13081 tgtaatttac ggagttatca catgtattcg ttatcccgca cacaaaaacc acgggcaggt
    13141 tttaccctgc tggaagtgat ggtggtgatt gttattcttg gcgtcctggc aagtctggtg
    13201 gtgcctaacc tgttgggcaa taaagagaaa gcggatcggc aaaaagccat cagcgatatc
    13261 gtggcgctgg agaatgcgct ggatatgtac cgactggata acgggcgtta tccgaccaca
    13321 gaacaggggc ttgaggcgct gatccagcag ccagccaata tggcggactc ccgcaactac
    13381 cgtactggtg gatacatcaa acgactgcca aaggatccgt ggggcaatga ttatcagtat
    13441 ctcagcccgg gtgaaaaagg actgtttgat gtttataccc tgggggcgga tggtcaggaa
    13501 aacggggaag gcgctggcgc agatatcggt aactggaatt tgcaggagtt tcagtaagca
    13561 gtgcctgaac gcggattcac acttctggaa atcatgttgg tgattttcct tatcggcctt
    13621 gccagttcgg gcgtggtaca gacgtttgcg accgattctg aaccgcccgc gaaaaaagcg
    13681 gcacaggatt ttctgactcg ctttgcgcag tttaaggaca gggcagtgat tgaagggcag
    13741 acactcggtg tgctaatcga cgcgcctggc tatcagttta tgcagcgtcg tcaggggcag
    13801 tggttgcccg tttccgcgac ccggttatcg gcacaggtta cggtgccaaa acaggtgcag
    13861 atgctgttac aacccggaag tgatatctgg cagaaggagt atgcgctgga gctgcaacgt
    13921 cgtcgcctga cgctgcacga tattgaactg gagctgcaaa aagaggcgaa aaagaagacg
    13981 ccgcagatcc gtttttcgcc ttttgaaccc gccacgccgt ttacgctgcg cttctactcg
    14041 gcggcgcaaa acgcatgttg ggcggtaaaa ctggcgcacg atggcgcgtt atccctcaat
    14101 caatgtgatg agaggatgcc atgaagcgcg gatttacctt gctggaagtg atgctcgcgc
    14161 tggcgttttt tgcgctggct gccacggcgg tgttacagat tgccagcggt gcgctgagta
    14221 atcagcaaat tcttgaggaa aaaacggtag cgggctgggt ggctgaaaac cagaccgcgc
    14281 tgctctacct gatgacccgc gagcagcggg cggtcaggca gcagggcgag agcgatatgg
    14341 caggaagccg ctggtactgg cgaaccacac cgctgagtac aggcaacgcg ctgctccagg
    14401 cggtggatat tgaagtcagc cttcacgaag acttttcgtc ggtgattcag tcacgacgcg
    14461 cctggtttag cgccgtggga ggccaacagt gagaaggact cgcgctggtt tcacgttact
    14521 ggaaatgttg gtggcaattg ccatttttgc ctcgctggca ctgatggcgc agcaggtgac
    14581 taacggcgtc acgcgcgtga atagcgccgt cgccggacac gatcaaaaac taaacctcat
    14641 gcagcaaacg atgagttttc tgacccacga tctgacccaa atgatgccgc gtccggtaag
    14701 aggcgatcag ggccagcgtg aaccagcgtt actggctggc gctggcgtgc tggcgtctga
    14761 gagtgaagga atgcgttttg tgcgcggcgg tgtggttaac ccgttgatgc gtctgccgcg
    14821 cagtaatctg ctcaccgtcg gttaccgcat tcatgacggt tatctcgaac ggttagcctg
    14881 gccgctgacc gatgccgcag gcagcgtgaa gccgacaatg caaaagttga ttccggccga
    14941 ttcgctccgt ttgcagttct acgacggcac gcgctggcag gagagctggt cgtcagtaca
    15001 ggcgatccct gtggcggtac gcatgaccct gcattcgccg caatggggcg agattgaacg
    15061 catctggttg ttacgcgggc cgcaataatc atgatcacct caccaccaaa acgcggaatg
    15121 gcactggttg tggtgctggt attgctggca gttatgatgc tggtaaccat cacgctttcc
    15181 gggcggatgc agcagcaact gggaagaacg cgcagccagc aggagtacca gcaggcgctg
    15241 tggtacagcg ccagtgcaga aagcctggcg ttgagcgcgc tcagtctgag cctgaaaaat
    15301 gaaaagcgcg tgcatctggc acaaccgtgg gcttccggcc ctcgtttttt cccactgccg
    15361 caggggcaaa tcgccgtcac tctgcgtgac gcacaggcct gctttaacct gaatgccctc
    15421 gctcagccca caacggcgtc gcgtccgctc gcggtacaac aactgattgc cctgatcacg
    15481 cgcctggatg tgcctgctta tcgggccgaa ctgatagccg aaagcctgtg ggagtttatt
    15541 gatgaagacc gcagcgtgca gacgcgtctg ggccgtgaag acagcgaata tctcgcccgc
    15601 tcggtgcctt tctacgccgc caatcaaccg ctggccgata tcagcgagat gcgcgtggtg
    15661 cagggaatgg acgccgggct ttatcaaaaa ctgaaaccgc tggtctgtgc gctgccgatg
    15721 acccgccagc aaatcaacat caatacttta gacgtcacgc aaagtgtgat tcttgaggcg
    15781 ctgtttgacc cgtggttaag ccctgttcag gcgcgggcgt tattacaaca acgtccggcg
    15841 aagggctggg aagatgtcga tcagtttctt gcacagccgc tacttgctga cgtcgatgag
    15901 cgtactaaaa aacagctaaa aaccgtcctg agcgtggaca gcaattactt ctggctgcgt
    15961 tcagatatca ccgtgaatga gattgaactg acgatgaact cgttaattgt ccgcatgggc
    16021 ccacaacact tttcggttct ctggcatcag acaggagaaa gtgagtgagt tccatccttg
    16081 agattttttt cccgctttgc gccgctgatc ccatccattg gcagcgccgt acacccgatg
    16141 tggagcacgg tatctggtct gacgttgcta acgaacagct ccagcaatgg ctgcaaactg
    16201 atgcgattcg actctacatt cccggcgaat ggatcagcgt ctggcaggtt gaactgcctg
    16261 ttgtcgcccg caagcagata ccgactattc tgcccgcctt actggaagaa gagctgaacc
    16321 aggatatcga tgaactgcat ttcgccccgt tgaaaatcga ccagcaactg gcaaccgtag
    16381 cagtgattca ccaacagcat atgcgcaaca ttgcgcagtg gttgcaggaa aacggcatca
    16441 cccgcgctac cgtcgcgccg gactggatgt ccattccttg tgggtttatg gctggcgatg
    16501 cgcaacgggt tatctgccgc atcgatgaat gccggggatg gagcgccggg ctggcgctgg
    16561 ctccggtcat gttccgcgcc cagctcaatg agcaggattt accgctttcg ctaaccgtag
    16621 tcggcattgc accggaaaag ctatcagcat gggctggcgc agacgctgaa cgcctgaccg
    16681 ttacagctct gcccgccgtt accacttatg gcgaaccgga agggaacctg ctgacagggc
    16741 cgtggcagcc tcgcgtcagc taccgaaaac agtgggcgcg ctggcgggtg atgattctgc
    16801 cgatattgct gattctggtt gcgctggcag tggagcgggg cgtgacgtta tggagcgtca
    16861 gcgaacaggt ggcgcaaagc cgcacccagg cggaggaaca gttcttaacg ttgttcccgg
    16921 agcagaagcg gattgtgaat ttacgctctc aggtgacgat ggcgctgaaa aaatatcgcc
    16981 cacaggccga cgatacccgg ctgctcgccg agttgtcagc gatagccagc accctgaaat
    17041 cagcgtcact ttccgacatc gaaatgcgtg gtttcacctt tgatcaaaaa cgccagacgc
    17101 ttcacctcca gttgcgggcc gcgaactttg ccagcttcga caaactgcgt agcgcactgg
    17161 cggcagattt tgttgtgcag caggacgcgt tacagaaaga gggtgatgcg gtttccggcg
    17221 gcgtaacgtt gcggaggaaa taacatgtta cgcgataaat ttattcacta ttttcagcaa
    17281 tggcgtgaac gccagttaag ccgtggcgaa cactggctgg cacaacacct ggcggggcgt
    17341 tctccgcgtg aaaagggcat gttactggca gcggtggtgt tcctgtttag cgtcggatat
    17401 tacgtcctca tctggcagcc tttgagcgaa cggattgagc aacaggagac aatattgcag
    17461 cagctggtgg cgatgaacac ccgactgaag agcgccgcgc cggatattat tgcggcgcga
    17521 aaatccgcca caacaacgcc tgcgcaggta tcgcgggtta tcagtgacag tgcttccgcg
    17581 cactcggtag tcatcaggcg gatagccgag cgtggggaga atattcaggt ctggatagaa
    17641 cctgtggtgt ttaatgacct gctgaaatgg ttaaacgcac tggatgaaaa atatgcgctg
    17701 cgggtgacac aaattgatgt cagtgctgct gagaagcctg ggatggtgaa tgtgcagcgg
    17761 ctggagtttg gacgggggta aagtatcagg agattaatat caataaaatt actctcgtta
    17821 acgtggcgag agtaattccc ccagacttta taagctaatt tttttcgtga aggttattgt
    17881 cagatccaaa agcaaaaacc cgccttgtgg gcgggttctt aagaatta
//
LOCUS       DAMZET010000084        17911 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.084, whole genome shotgun sequence.
ACCESSION   DAMZET010000084 DAMZET010000000
VERSION     DAMZET010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.084"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..619)
                     /locus_tag="POB34_002871"
     CDS             complement(<1..619)
                     /locus_tag="POB34_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
                     /protein_id="HDI5919778.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAV"
     gene            complement(738..1184)
                     /gene="hpaR"
                     /locus_tag="POB34_002872"
     CDS             complement(738..1184)
                     /gene="hpaR"
                     /locus_tag="POB34_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HDI5919779.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            1456..2745
                     /locus_tag="POB34_002873"
     CDS             1456..2745
                     /locus_tag="POB34_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HDI5919780.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARSVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            2742..4208
                     /gene="hpaE"
                     /locus_tag="POB34_002874"
     CDS             2742..4208
                     /gene="hpaE"
                     /locus_tag="POB34_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HDI5919781.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            4210..5061
                     /gene="hpaD"
                     /locus_tag="POB34_002875"
     CDS             4210..5061
                     /gene="hpaD"
                     /locus_tag="POB34_002875"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HDI5919782.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            5071..5451
                     /locus_tag="POB34_002876"
     CDS             5071..5451
                     /locus_tag="POB34_002876"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HDI5919783.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            5519..6322
                     /gene="hpaH"
                     /locus_tag="POB34_002877"
     CDS             5519..6322
                     /gene="hpaH"
                     /locus_tag="POB34_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HDI5919784.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            6333..7121
                     /gene="hpaI"
                     /locus_tag="POB34_002878"
     CDS             6333..7121
                     /gene="hpaI"
                     /locus_tag="POB34_002878"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HDI5919785.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            7296..8672
                     /gene="hpaX"
                     /locus_tag="POB34_002879"
     CDS             7296..8672
                     /gene="hpaX"
                     /locus_tag="POB34_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HDI5919786.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            8682..9572
                     /gene="hpaA"
                     /locus_tag="POB34_002880"
     CDS             8682..9572
                     /gene="hpaA"
                     /locus_tag="POB34_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HDI5919787.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFHMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            9823..11385
                     /gene="hpaB"
                     /locus_tag="POB34_002881"
     CDS             9823..11385
                     /gene="hpaB"
                     /locus_tag="POB34_002881"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HDI5919788.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            11403..11915
                     /locus_tag="POB34_002882"
     CDS             11403..11915
                     /locus_tag="POB34_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HDI5919789.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            12308..14458
                     /gene="btsT"
                     /locus_tag="POB34_002883"
     CDS             12308..14458
                     /gene="btsT"
                     /locus_tag="POB34_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HDI5919790.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            14508..14711
                     /locus_tag="POB34_002884"
     CDS             14508..14711
                     /locus_tag="POB34_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDI5919791.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            14722..15678
                     /gene="yjiA"
                     /locus_tag="POB34_002885"
     CDS             14722..15678
                     /gene="yjiA"
                     /locus_tag="POB34_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HDI5919792.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(15756..>17911)
                     /locus_tag="POB34_002886"
     CDS             complement(15756..>17911)
                     /locus_tag="POB34_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HDI5919793.1"
                     /translation="IDKVNTNPETLLPKQFNTFGFTPKRWEPFDVAMIFVGTMANRFS
                     DSTSEIDNLALLTALKDKYGVSQGMAVFNQLKWLVNPSAPTTIAVQESSYPLKFNQQN
                     SQTAALLPRYDLPAPMLDRPAKGADGALLALTAGKNRETIAAQFAQGGANGLAGYPTT
                     SNMWVIGKSKAQDAKAIMVNGPQFGWYAPAYTYGIGLHGAGYDVTGNTPFAYPGLVFG
                     HNGVISWGSTAGFGDDVDIFAERLSAEKPGYYLHNGKWVKMLSREETITVKNGQAETF
                     TVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAKNWQEWTQQAAKQA
                     LTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKGLLPFEMNPKVYNP
                     LSGYIANWNNSPQKDYPASDLFAFLWGGADRVTEIDRLLEQKPRLTADQAWDVIRQTS
                     RQDLNLRLFLPTLQAATSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILN
                     VWLTSMLKRTVVAAVPMPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSP
                     IPQAVDLFAGKPQQEVVLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQ
                     AAAEETRHQAEYQNRGTENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDKHYE
                     DQLKMYENFGRKSLWLTKQDVEAHKESQEVLHVQR"
BASE COUNT         4189 a         4864 c         4719 g         4139 t
ORIGIN      
        1 tgaccgccag tacgacgatc atcacgccca ccagaatcca catcgcctgg ctgtaggagg
       61 cattgttatc gctgacggcg atatcataga gccgatcgtt ttgctccatg taagccacat
      121 actgcttctc gaaaccgtcc tgatatccct gagtcggctg atcaaagaac tcgttgattt
      181 tgcctgcacc taacagttgg atcagctccg ccagcgcatt gtgataaata tcgtaattac
      241 gtttgatctc tgccgctgcg gcggtgctct gacgcgggtc acgcggcaac gcttcgtaat
      301 ccgcccagtt tttttccgcc tgtttcagcg aaatactggc actctgcatc agctcagcaa
      361 cggttgaacc gctaccaata ttattctgat ccatcatgta gcggataccc gcgcggttga
      421 gggtgttacg cgtctgcaac aacgcgaccc agctgccatt cagcgtggat tgctgctggc
      481 gaatggtttg taaaacagtg aaattttctt tgtcattctt taaggcatta aagaacagac
      541 cgcctgatgt cagttgtaaa aggccaaaaa cggccaaaac cagcagtaag ctggtcacga
      601 tcttgatacg ttttaacatg gtttctcttt cctgtggacc gatgcaagat tttcggcctg
      661 gaaagcaaaa actttatgaa aaggtgtaac ccgccatcca tggaaaaacc aggcgctggt
      721 tatcagatac taaaaagtta ttcattatcg cccgggatgg cttcttgtcg ggaattgccc
      781 agagcaataa attcctctaa cagatgggtt aactgttgca ttttctcggc agtaaactga
      841 gcttcaatct gccgataggc ttcttcaatc tgtgtctgag cgcggttata cagcgcctgc
      901 ccctctttgg ttagcgagat atagagcttc cgctgatcat tgatcggctt taatcgcaac
      961 actaaaccgt cgcgttccat ccgcgtaagg atcccggtca ggcttgggcg caaaatgcag
     1021 gcgcgatacg ccagatcgtg aaaatccatt gatgggcttt ccgccagaat acgcacgatg
     1081 cgccactgtt gctcagtcaa attatgccgt ttaacgattg ggcgaaaata actcatcgcc
     1141 gcttctcgcg cctggagcaa cgcaatggtt agagagtcgt gcattatctt tcccctgaaa
     1201 aattcaaaat cattaatata gaaacagtta atcatcacgt taccgggact gcgctgcccc
     1261 tgcaattctt cattttatca aaattgagca tgttttgtta acttgctgat atttaatatt
     1321 ttattatctt taaatgtaaa tattttgtta attagatcac atttacatca cttacttttg
     1381 ccaaactacg caatcgggaa taaaagtaat cattaacata ttaatgatta aaatgatcga
     1441 atccgaggag tggtaatgaa aggcactatc ttcgccgtag cgttgaacca tcgcagccaa
     1501 cttgatgcat ggcaggaagc gttccagcaa tccccctaca aagccccgcc taaaactgcg
     1561 gtctggttta ttaaaccgcg caatacggtg attggttgcg gtgaaccgat tccctttcca
     1621 cagggtgaaa aggtactgag cggtgcgact gttgcgctga ttgtgggaaa aacggcgacg
     1681 aaagtacgtg aagaagatgc ggcagagtac atcgccggat atgcgctggc taacgacgtc
     1741 agcctgccgg aagagagctt ttaccgcccg gcaatcaaag caaaatgtcg tgatggattc
     1801 tgccccattg gcgaaaccgt ggctctcagc aatgtcgata atctgaccat ctataccgag
     1861 atcaacgggc gtcctgccga tcactggaat accgccgatt tacaacgtaa cgccgcgcag
     1921 ttgctgagcg ccctgagcga atttgccaca ctgaatccag gcgatgccat tctgctcggc
     1981 acgccacagg cgcgcgtgga aatacagcca ggcgatcgcg ttcgtgttct cgcagaaggt
     2041 ttcccgccgc tggaaaatcc ggtagtggac gaacgtgaag tgaccacgcg caagagcttc
     2101 ccaacgcagc cacacccgca cggcacgctg tttgccctcg gcctgaacta cgccgaccac
     2161 gccagcgaac tggaatttaa gccaccggaa gaaccgctgg tgttcctgaa agcgccaaat
     2221 accctcactg gcgataacca gacctccgtg cgtccaaaca atattgaata catgcactat
     2281 gaagcggagc tggtggtagt tattggcaaa caggcgcgta gcgtcagcga agccgatgcc
     2341 atggattatg tcgcgggcta caccgtgtgt aacgactacg ccattcgcga ctatctggaa
     2401 aactactacc gccctaacct gcgggtaaaa agccgcgacg gactgacgcc gatgctttca
     2461 accatcgtgc cgaaagaggc gatcccggac ccgcataatc tgacccttcg caccttcgtc
     2521 aacggcgagt tacgccagca aggcaccacc gccgatctga tcttcagcgt gcccttcctg
     2581 atcgcctact taagcgaatt tatgaccctg aatccgggcg acatgatcgc caccggcaca
     2641 ccaaaaggct tatctgacgt ggtgcctggt gatgaagtag tggtggaagt agaaggcgtg
     2701 ggccgcctgg tgaaccgaat tgtgagtgag gatacagcga aatgaaaaaa gtaaatcatt
     2761 ggatcaacgg caaaaatgtc gcaggtaacg actacttcca gaccaccaat ccggcaacgg
     2821 gtgaagtgct ggcggatgtg gcctctggcg gtgaagcgga gatcaatcag gcggtagcgg
     2881 cagcgaaaga ggcgttcccg aaatgggcca atctgccgat gaaagagcgt gcgcgcctga
     2941 tgcgccgtct gggcgatctg atcgaccaga acgtgcctga gatcgccgcg atggaaaccg
     3001 ccgacacggg cctgccaatc catcagacca aaaatgtgtt gatcccacgc gcttcccaca
     3061 actttgaatt tttcgcggaa gtctgccagc agatgaacgg caagacttat ccggtcgacg
     3121 acaagatgct caactacacg ctggtgcagc cggtaggcgt ttgtgcactg gtgtcaccgt
     3181 ggaacgtgcc gtttatgacc gccacctgga aggtcgcgcc gtgtctggcg ctgggcaata
     3241 ccgcggtact gaaaatgtcg gaactctccc cgctgaccgc tgaccgcctg ggtgagctgg
     3301 cgctggaagc tggtattccg gcgggcgtgc tgaacgtggt acagggctac ggcgcaaccg
     3361 caggcgatgc gctggtccgt catcatgacg tgcgtgccgt gtcgttcacc ggcggtacgg
     3421 cgaccgggcg caacatcatg aaaaacgccg ggctgaaaaa atactccatg gaactgggcg
     3481 gtaaatcgcc ggtgctgatt tttgaagatg ctgatattga acgcgcgctg gacgccgccc
     3541 tgttcaccat cttctcgatc aacggcgaac gctgcaccgc cggttcgcgc atctttattc
     3601 agcaaagcat ctacccggaa ttcgttaaac gctttgccga acgcgccaac cgtctgcgcg
     3661 tgggcgatcc gaacgatccg aatacccagg ttggcgcgct tatcagccag cagcactggg
     3721 aaaaagtctc cggctatatc cgtctcggca ttgaagaagg cgcaaccctg ctggcgggcg
     3781 gcccggataa accgtccgac ctgcctgcgc acctgaaagg cggcaacttc ctgcgcccaa
     3841 ccgtgctggc agacgttgat aaccgtatgc gcgtcgccca ggaagagatt ttcgggccgg
     3901 tcgcctgcct gctgccgttt aaagacgaag ccgaaggctt acgtctggca aacgacgtgg
     3961 agtacggcct cgcgtcgtac atctggacac aggatgtcag caaagtgtta cgcctggcgc
     4021 gtggcattga agctggcatg gtgttcgtca acacccagaa cgtgcgtgac ctgcgccagc
     4081 catttggcgg cgtaaaagcc tccggcaccg ggcgtgaagg cggtgagtac agcttcgaag
     4141 tgttcgcgga aatgaagaac gtctgcattt ccatgggcga ccatccaatt ccgaaatggg
     4201 gagtctgata tgggtaagtt agcgttagcc gccaagatca cgcacgtacc gtcgatgtat
     4261 ctctctgaac tgccagggaa aaaccacggt tgccgccagg gcgcgatcga cgggcataaa
     4321 gagatcagca agcgttgccg ggaaatgggc gtcgatacca ttatcgtttt cgatacccac
     4381 tggctggtca acagtgctta tcacatcaac tgtgcagacc attttgaagg cgtctacacc
     4441 agcaacgagc tgccgcattt tatccgcgat atgacctaca actacgaggg caacccggag
     4501 ttggggcagc ttattgccga tgaagcctta aagctcggcg tgcgggcaaa agcgcacaac
     4561 attcccagcc tgaaactgga atacggcacg ctggtgccaa tgcgctacat gaatgaagac
     4621 aagcacttca aagtggtttc catttcagct ttctgcactg ttcacgattt tgccgacagc
     4681 cgcaagctgg gcgaggcgat tctgaaagcg atcgaacagt acgacggcac cgtggcggtc
     4741 cttgccagcg gttcgttatc gcaccgcttt attgacgatc agcgagcgga agaagggatg
     4801 aacagctaca cccgcgagtt cgaccgccag atggacgagc gtgtggtgaa gctgtggcgc
     4861 gaaggccagt tcaaagagtt ctgcaatatg ctgccggagt acgccgacta ctgctacggc
     4921 gaaggcaata tgcacgacac ggtgatgctg ctggggatgc tcggctggga taaatacgac
     4981 ggcaaggtgg agtttatcac cgagctattc ccaagctctg gcaccggtca ggttaacgct
     5041 gttttcccgc ttcccgcgta aggacgtttt atgccgcact ttatcgtgga atgtactgaa
     5101 aatattcgtg aagaagcccg cttacctgag ctgtttgcca gtgttaacac tgcactggcg
     5161 gcaacgggga tttttccgct tgggggaata cgtagccggg cgcactggat cgatacctgg
     5221 caaatggctg atggcaagca cgattacgcc tttgtgcata tgacgctaaa aatcggcagc
     5281 gggcgcagcc tggaaagccg tcaggaagtg ggcgaaatgc tgtttgatct gattaaaacg
     5341 cacttcgcct cactgatgga gagccgttac ctggcgcttt catttgagat tgcagagctg
     5401 cacccgacgc tgaattttaa acaaaacaac gtgcacgcgt tgtttaaata aatttgcacc
     5461 agcttccagg ccggatgata aggcgcagcc gcatccggca atgccacagg atatcgctat
     5521 gttcgataaa cacacccaca ccctgatcgc ccagcgtctg gatcaggcag aaaaacagcg
     5581 cgaacagatc cgcgcgatct cgctggatta cccggagatc accatcgaag acgcttacgc
     5641 ggtgcagcgt gaatgggttc gactgaaaat cgccgaaggt cgcacgctga aaggccacaa
     5701 aatcggcctg acctcgaaag cgatgcaggc cagctcacaa atcagcgagc cggattacgg
     5761 tgccctgctg gatgacatgt tcttccacga tggcagcgat atcccgaccg atcgctttat
     5821 cgtgccgcgc attgaagtgg agctggcttt cgtgctggca aaaccgctgc gtggaccaaa
     5881 ctgcacgctt ttcgacgtct acaacgccac ggactacgtg atcccggcgc tggagctgat
     5941 cgacgctcgc tgccacaaca tcgatccgga aacccagcgc ccgcgcaaag tgttcgacac
     6001 catttctgat aacgccgcca acgccggggt gatcctcggt ggtcgtccca ttaagcccga
     6061 tgagttggat ctgcgttgga tctccgccct gatgtatcgc aatggcgtga ttgaagaaac
     6121 cggcgtcgcc gctggtgtgc taaatcatcc ggcaaacggc gtggcctggc tggcgaacaa
     6181 actcgcaccg tatgacgtac aactggaagc cgggcaaatc attctcggcg gttcgttcac
     6241 ccgcccggtt ccggcgcgta agggcgacac cttccacgtc gattacggca acatgggctc
     6301 cattagctgc cgctttgttt aaggagagaa cgatggaaaa cagttttaaa gcggcgctga
     6361 aagcaggccg cccgcagatc ggattatggc tggggctgag tagcagctac agcgcagagt
     6421 tactggccgg agcaggattc gactggttgt tgatcgacgg tgagcacgcg ccgaataacg
     6481 tgcaaaccgt gctcacccag ctacaggcga ttgcgcccta ccccagccag ccggtagtac
     6541 gtccgtcgtg gaacgatccg gtgcaaatca aacaactgct ggacgtcggc acacaaacct
     6601 tgctggtgcc gatggtacaa aacgctgacg aagcccgtga agcggtacgc gccacccgtt
     6661 atccccccgc cggtattcgc ggtgtgggca gtgcgctggc ccgcgcctcg cgctggaatc
     6721 gtattcctga ttacctgcaa aaagccaacg atcaaatgtg cgtgctggtg cagatcgaaa
     6781 cgcgtgaggc aatgaagaac ttaccgcaga ttctggacgt ggaaggcgtc gacggcgtgt
     6841 ttatcggccc ggcggatctg agcgccgata tgggttatgc cggtaatccg cagcacccgg
     6901 aagtacaggc cgccattgag caggcgatcg tgcagatccg tgaatcgggc aaagcgccgg
     6961 ggatcctgat cgccaatgag caactggcaa aacgctatct ggaactgggc gcgctgtttg
     7021 tcgccgtcgg cgttgacacc accctgctcg cccgcgccgc tgaagcgctg gcagcacggt
     7081 ttggtgcgca ggccaccgcc gtgaagcccg gcgtgtatta atgccggatg cgctgcactt
     7141 atccggccta cactcgcacc aaaccgtagg ccggataagg cgtttacgcc gcatccggca
     7201 aaaagccgta ccaaatcgcg gaccggataa ggcgtttgtg cgcatccgcc ataaaccttg
     7261 tcgtacccta caaaaatccc attagaggaa gaaaaatgag cgacacctca cctgccatac
     7321 cggagagtat cgatccggcg aatcagcata aagcgctgac tgccggacaa caggcggtta
     7381 ttaagaagct atttcgccgc ctgatcgtct ttctgttcgt gctgtttatc ttctcgttcc
     7441 ttgatcgcat caacatcggc tttgccggac tcacgatggg acgcgacctc ggtctgagcg
     7501 ccaccatgtt tggcctcgct accaccctgt tctatgccgc ttatgtcatc ttcggcattc
     7561 ccagcaacat tatgctgagt attgtcggtg cgcggcgctg gatcgccacc atcatggtgc
     7621 tctggggcat cgcctctact gccaccatgt ttgccactgg ccccaccagc ttgtacgtac
     7681 tgcgtatact ggttggcatt accgaagccg gctttctgcc tggtattctg ctgtatttaa
     7741 ccttctggtt tccggcctat ttccgcgccc gtgccaacgc cttgtttatg gtagcaatgc
     7801 cggtaacgac agcgttgggc tcgatcgttt ccggctacat tttgtcgctg gatggcgtca
     7861 tggcattaaa aggctggcag tggctgtttt tgctggaagg cttcccgtcg gtattactcg
     7921 gcgtcatggt gtggttctgg cttgatgact caccggacaa agctaagtgg ctgacgaaag
     7981 aagacaaaaa atgcctgcaa gagatgatgg ataacgatcg tctgacgctg gttcagccag
     8041 agggagccat cagccaccac gccatgcaac aacgcagtat gtggcgggag atcttcactc
     8101 cggtggtgat gatgtatacc ctagcgtatt tctgcctgac caacacactt agtgcgatca
     8161 gcatctggac accgcagatc ctgcaaagtt ttaatcaggg cagcagtaat atcaccatcg
     8221 gcctgctggc cgccgtaccg cagatttgta ccattctcgg gatggtctac tggagccgtc
     8281 actcagatcg ccgccaggaa cgaaggcatc acaccgccct tccttatttg ttcgctgccg
     8341 caggttggtt actggcttcg gcaactgatc acaacatgat ccagatgctg gggatcatta
     8401 tggcttcgac cggatcattc agcgcaatgg cgattttctg gacaacaccg gatcagtcca
     8461 tcagcctgcg ggcacgagcg atcggtattg cggtgatcaa cgccactggc aacattggtt
     8521 cagcgttaag tccgtttatg atcggctggt tgaaagattt gaccggcagc tttaacagtg
     8581 gattgtggtt tgttgccgcg ctgctggtga ttggtgcggg gattatctgg gcgattccaa
     8641 tgcagtcctc ccgtccgcga gcgaccccgt aaggaacgac gatgtgtgac cgtcagattg
     8701 ccaatattga tatcagcaaa gagtacgatg aaagcctggg cacggacgat gtgcattatc
     8761 agtcgttcgc ccgcatggcg gccttttttg gccgccatat gctgccacat cgccacgaac
     8821 agtactttca gatgcatttc ctcaatagcg gacagattga gctacagctt gacgatcatc
     8881 gctactcggt ggaagcgccc ctgtttgtcc tgacgccgcc gtcagtacct catgcgttta
     8941 ttacggagtc tgatgctgac ggtcatgtat tgacggtacg ggaagatctg atctggcccc
     9001 tgctggaagt tctttatccg ggcactcggg aaaccttcgg cctgccgggg atttgcctgt
     9061 cactggcaga taaacccgac gaactggcgg cgctggaaca ctattggcaa ctgatagagc
     9121 gggaatcggt agaacaactg cctggacggg aacacaccct gacgttactg gcacaggcag
     9181 tgttcaccct actgctgcgt aacgcaaaac tcgacgacca tgccgccagc ggaatgcgcg
     9241 gagaattaaa actgttccag cgttttcata tgcttattga aagccatttt catcagcact
     9301 ggacagtacc ggattacgct aacgaactgc atatcaccga atcacgcctc acggacatct
     9361 gccgccgctt tgccaaccgt ccgccaaaac ggttgatttt cgacaggcag ctacgagaag
     9421 ccaagcggct gctgctgttt tctgataacg ccgtgaacaa tattgcctgg caactcggtt
     9481 ttaaggatcc ggcttatttt gcgcgctttt ttaatcgctt agtcggttgc tcgcccagtg
     9541 cttatcgtgc caaaaaagta cctgtgacgt gaaattccct tagccttaac gggaaaccag
     9601 gcaccacctg ctattcccct ttcttcgcct ggagcaaggg gatttagtaa cgttaaaaat
     9661 cgctaaaagc gacctcgatc acaaaaccgt acccgacctg aaaagtacca gccattgtcc
     9721 caaaagtgtc ttcccgtaat cgcccctgca agcttcaatt gaataacaaa aacaacacac
     9781 aaaattaaca atcgtattcc gattaatact gtagaggtcg acatgaaacc agaagatttc
     9841 cgcgccagta cccaacgtcc gttcaccggg gaagagtatc tgaaaagcct gcaggatggt
     9901 cgcgagatct atatctatgg cgagcgagtg aaagacgtca ccactcatcc ggcatttcgt
     9961 aatgcggcag cgtctgttgc ccaactgtac gacgcgctac acaaaccgga gatgcaggac
    10021 tctctgtgct ggaacaccga caccggtagc ggcggctata cccataaatt cttccgcgtg
    10081 gcgaaaagtg ccgacgacct gcgccagcaa cgcgacgcca tcgctgagtg gtcacgcctg
    10141 agctatggct ggatgggccg taccccagac tacaaagccg ctttcggttg cgcactgggc
    10201 gcgaatccgg gcttttacgg tcagttcgag cagaacgccc gtaactggta tacccgtatt
    10261 caggaaactg gcctctactt taaccacgcg attgttaacc caccgatcga tcgtcatttg
    10321 ccgaccgata aagtgaaaga cgtttacatc aagctggaaa aagagactga cgccgggatt
    10381 atcgtcagcg gtgcgaaagt ggttgccacc aactcggcgc tgactcacta caacatgatt
    10441 ggcttcggct cggcacaagt gatgggcgaa aacccggact tcgcactgat gttcgttgcg
    10501 ccaatggatg ccgatggcgt gaaattaatc tcccgcgcct cttatgagat ggtcgcgggt
    10561 gctaccggct cgccgtacga ctacccgctc tccagccgct ttgatgagaa tgatgcgatt
    10621 ctggtgatgg ataacgtgct gatcccatgg gaaaacgtac tgatctaccg cgatttcgat
    10681 cgctgccgtc gctggacaat ggaaggcggt tttgcccgta tgtatccgct gcaagcctgt
    10741 gtgcgcctgg cagtgaaact cgacttcatt acagcactgc tgaaaaaatc actcgaatgt
    10801 accggcaccc tggagttccg tggtgtacag gccgatctcg gtgaagtggt ggcgtggcgc
    10861 aacaccttct gggcattgag tgactcgatg tgttctgaag cgacgccgtg ggtcaacggg
    10921 gcttatttac cggatcatgc cgcactgcaa acctatcgcg tactggcacc aatggcctac
    10981 gcgaagatca aaaacattat cgaacgcaac gttaccagtg gcctgatcta tctcccttcc
    11041 agtgcccgtg acctgaacaa tccgcagatc gaccagtatc tggcgaagta tgtgcgcggt
    11101 tcgaatggta tggatcacgt ccagcgcatc aagatcctca aactgatgtg ggacgccatt
    11161 ggcagcgagt ttggtggtcg ccacgaactg tatgaaatca actactccgg tagccaggat
    11221 gagattcgcc tacagtgtct gcgccaggca caaagctccg gcaatatgga caagatgatg
    11281 gcgatggttg atcgctgcct gtcggaatac gaccagaacg gctggactgt gccgcacttg
    11341 cacaacaacg acgatatcaa catgctggat aagctgctga aataacgcag caggaggtta
    11401 agatgcaatt agatgaacag cgcctgcgct ttcgtgacgc gatggccagc ctgtcggcag
    11461 cggtaaatat tatcaccacc gagggcgacg ccggacaatg cgggattacg gcaacggccg
    11521 tctgctcggt cacggataca ccaccatcgc tgatggtgtg cattaacgcc aacagtgcga
    11581 tgaacccggt ttttcagggc aacggtaagt tgtgcgtcaa cgtcctcaac catgagcagg
    11641 aactgatggc acgccacttc gcgggcatga caggcatggc gatggaagag cgttttagcc
    11701 tctcatgctg gcaaaaaggt ccgctggcgc agccggtgct gaaaggttcg ctggccagtc
    11761 ttgaaggtga gatccgcgat gtgcaggcaa ttggcacaca tctggtgtat ctggtggaga
    11821 ttaaaaacat catcctcagt gcagaaggtc acggacttat ctactttaaa cgccgtttcc
    11881 atccggtgat gctggaaatg gaagctgcga tttaagctcc agccccttca cgcattcgtc
    11941 gaaggggcat ttttattgtg atctcaatca catatccacc cgatgaataa ttcttgccca
    12001 accgctcacc gccattcctg accgcttacg tccctaaaat aaccactcag ttatttacct
    12061 tactttacgc gcgcgtaact ctggcaacat cactgcagga tagcggtcaa tttacctcct
    12121 caaacgcaac gcaaacctag aacggcttcg gcctatgatt aacaaattac tctatgaatt
    12181 ttggtttgca ggaaggcagc aagtgagtga atccccggga gcttacaaca gtaagtgaca
    12241 ggggtgaacg aacgcaactg ccgcacctgt aagccaaaag acgacgagta aaatgccagg
    12301 ttttactatg gatacgaaaa aactattcaa gcacataccc tgggtgattc tcggaatcat
    12361 cggtgcattc tgcctcgcgg tagttgcatt acgtcggggg gagcacgtca gcgccctgtg
    12421 gatcgtggtc gcctctgtgt cggtgtatct ggtggcgtat cgctactaca gtctgtacat
    12481 cgcccagaag gtgatgaaac tcgaccccac gcgcgcgacg cctgcggtta ttaacaacga
    12541 cggtctgaac tacgttccga ccaaccgtta cgtgttgttt ggtcaccact tcgccgctat
    12601 cgccggtgct ggtccgctgg tcggtccggt tctcgccgca cagatgggct acctgcctgg
    12661 cacgctgtgg ctgctggcgg gggtagtgct ggccggtgcg gtacaggact ttatggtgct
    12721 gtttatctcc tctcgccgta acggcgcatc tctcggtgag atgatcaaag aagagatggg
    12781 gccagtaccg gggactatcg cgctgtttgg ctgtttctta atcatgatca tcatccttgc
    12841 cgtcctggcg ctgattgtgg ttaaagccct ggccgaaagt ccgtggggtg tcttcaccgt
    12901 ttgctcaacc gtaccgattg cgctgtttat gggtatctac atgcgcttta tccgtccggg
    12961 gcgtgtgggt gaagtctctg tcattggtat cgtgctgctg gttgcctcta tctacttcgg
    13021 tggcgtgatt gctcacgatc cgtactgggg tccggcactg acctttaaag acaccaccat
    13081 taccttcgcg ctgattggct atgcgtttgt ttccgcattg ctgccggtgt ggctgatcct
    13141 cgcaccgcgc gactatctgg caaccttcct gaaaatcggc gttatcgtcg gcctggcgct
    13201 gggtatcgtg gtgctgaacc cggaactgaa aatgcctgcg atgacccagt acattgacgg
    13261 tactggcccg ctgtggaaag gtgctctgtt cccgttcttg ttcattacca tcgcctgtgg
    13321 tgcggtatct ggcttccacg cactgatctc ttccggtacg acgccgaaac tgctggctaa
    13381 tgaaaccgac gcgcgtttca ttggctacgg cgcaatgctg atggagtcct tcgtggcgat
    13441 tatggcgctg gttgctgcgt ccatcatcga accgggtctt tacttcgcga tgaacacccc
    13501 gcctgctggc cttggcatca ccatgcctaa cctgcatgaa atgggtggcg agaacgcgcc
    13561 gatcatcatg gcgcagctga aagacgttac cgcacacgcg gcagcgaccg tcagctcctg
    13621 gggcttcgtg atttcgccag agcagatcct gcaaaccgcg aaagacattg gtgagccttc
    13681 tgtcctgaac cgtgcaggtg gtgcgccaac gctggcggta ggtatcgctc acgtgttcca
    13741 caaagtgctg ccgatggctg acatgggctt ctggtatcac ttcggtattc tgttcgaagc
    13801 cctgttcatc ctgaccgcgc tggatgcggg tactcgttct ggccgcttta tgctgcaaga
    13861 cctgctgggt aacttcatcc cgttcctgaa aaaaaccgac tctctggttg ccggtatcat
    13921 cggtactgcg ggctgtgtgg gtctgtgggg ctacctgctg tatcagggcg tggtcgatcc
    13981 gctgggcggc gttaagagcc tgtggccgct gttcggtatt tccaaccaga tgctggcagc
    14041 cgtagcgctg gtactgggca ccgttgtgct gattaagatg aagcgcaccc aatacatctg
    14101 ggtaactgtt gttccggctg tatggctgct tatctgcacc acctgggcgc tgggcctgaa
    14161 actgttcagc accaacccgc agatggaagg cttcttctac atggcaagcc agtacaaaga
    14221 gaagattgct aacggtactg acctgacggc gcagcagatt gccaacatga accacatcgt
    14281 tgtaaacaac tacaccaacg caggcctgag tattctgttc ctgattgtgg tgtacagcat
    14341 catcttctac ggtttcaaaa cctggcttgc ggtgcgtaac agcgacaaac gtactgacaa
    14401 agaaactccg tacgttccaa tcccggaagg cggcgtgaag atctcttcgc accactaacc
    14461 gtgtttagcc ccgcttcggc ggggctttgt tcttatcaga gtgaactatg tttggtaact
    14521 taggacaggc aaaaaaatat ctcggccagg cggcgaagat gttgattggc attccagact
    14581 atgacaacta cgtcgagcat atgaagacca accatcccga caagccgtac atgagctatg
    14641 aagaattctt ccgcgaacgc cagaacgcac gctacggcgg cgatggtaaa ggcggtatgc
    14701 gctgttgtta atggagagag catgaacccg attgcagtta ccctactcac cggtttttta
    14761 ggcgcgggca aaaccaccct gctgcgccat attcttaatg aacaacacgg ctacaagatt
    14821 gccgtgattg aaaacgaatt cggcgaagtc tccgttgatg atcaattgat tggtgatcgc
    14881 gcaacgcaga tcaaaacgct gaccaacggc tgcatctgct gttcgcgctc caacgagctg
    14941 gaggacgcgc tactcgacct gctggataat ctcgacaagg gcaatattca gttcgatcgt
    15001 ctggtcattg aatgcaccgg catggccgat cctggcccga ttattcaaac ctttttctcc
    15061 catgagattt tatgccagcg ctacctgctg gacggcgtga ttgcgctggt ggatgcggta
    15121 catgccgatg agcagatgaa ccagttcacc atcgcccagt cccaggtggg ctacgccgac
    15181 cgtattctgc tgactaaaac cgacgtcgca ggcgaagcag aaaaactgcg tgaacgtctg
    15241 gcacgcatca acgcccgtgc accggtctac accgtcaccc acggcgacat cgacctgggt
    15301 ctgctgttca acaccaacgg ttttatgctg gaagaaaacg tcgtcagcac caaaccgcgt
    15361 ttccatttta tcgcggacaa acaaaacgat atttcgtcga ttgtggtgga actggattac
    15421 ccggtagata tcagcgaagt ttcccgggtg atggaaaacc tgctgctgga atcggcggat
    15481 aaactgctgc gttacaaagg gatgctgtgg attgacggcg aacctaaccg cctgctgttc
    15541 cagggcgtcc agcgcctcta cagcgccgac tgggacaggc catggggcga tgaaaaaccg
    15601 catagcacga tggtgtttat cgggattcag ttgccggagg atgagattcg ggcggcgttt
    15661 gcgggactca aaaagtaacc agccctccaa catcacaata cctgagactt tatcaccctc
    15721 atattcctcc agaatatgag ggcattcggg cttaattatc tctgaacgtg caacacttcc
    15781 tgcgactcct tatgcgcctc cacatcctgc ttcgttaacc agagcgactt acgaccaaaa
    15841 ttttcgtaca ttttcagctg atcttcatag tgcttatcaa ctgttccatc gggagcaata
    15901 aacccactct gaccgggtgc gaccacatcc caggcaagca caggacgatc gcttgtcgtt
    15961 ggtgagaaaa caatcatatc gttttctgtt ccacggtttt gatactccgc ctgatgacgc
    16021 gtttcttccg ctgcggcctg cggtacacca aagaaattat ttgcccggaa cgttaaggcc
    16081 attgcaggtg ttttccagtt actcacatta ttgccatagc gtttggaaag agtctcccag
    16141 gtatcttcca gcgcagccaa cacaacctcc tgctgtggtt tcccagcaaa cagatcaacc
    16201 gcttgtggga ttggtgattt gtctccctgc accgcctcat acaaaatttt tgctccaaca
    16261 cttatattca gcgaaccagt tgggccgtcc tgggttgttt cgtagccact ggcgctgtac
    16321 cacttatcaa atggcatagg tacggcagcc actacggtac gcttcaacat actggtcagc
    16381 caaacgttca ggatggcaga gcctggctgc tgccaggttt taccatcatc attaagcaaa
    16441 ttgatgccat cccaacgtgt taatgtatcc accaactgac ggcgcggatc gctctgtgtc
    16501 aaaccagatg tcgctgcttg cagagtaggt aaaaaaagcc tcaggttaag atcctgacga
    16561 ctggtttggc gaataacatc ccatgcctga tcagcagtta agcgtggctt ttgctcaagc
    16621 agtcggtcga tctccgtaac gcgatctgcc cccccccaca aaaaggcaaa cagatccgaa
    16681 gcgggataat ctttttgggg agaattgttc cagttagcaa tatatcccga cagggggtta
    16741 tacaccttag ggttcatttc aaaaggcaat agccctttcc agtcccattt tcccgtacca
    16801 ggaacgggta atcgcggatc atggcctgat tgacgatctg gataagcacc agtatgaaca
    16861 taaccaatat tgccgtttac atcagcatag taccagttga tggtcagtgc ttgtttcgct
    16921 gcctgctgtg tccactcctg ccaatttttg gccttcatct gatgagtcca ggccagcaaa
    16981 gacgccacct ctttaccatc ccatgcgcgg gatttagcgt aagccgtttg tgtcgtctgg
    17041 tcagtttgga gaatgttgcc atgcaccgta cgccagacag taaaggtctc tgcctgacca
    17101 tttttcaccg taatggtttc ctcacggctt aacattttca cccacttacc attatgcaag
    17161 tagtagcctg gtttctctgc cgacagccgt tcagcaaaaa tatcgacatc atcgccgaaa
    17221 cctgccgttg atccccagga aatcacacca ttatgaccaa aaaccagccc aggataggca
    17281 aatggtgtat tgccagtgac atcataacca gcaccgtgca gaccaatacc ataagtatac
    17341 gcaggcgcat accagccaaa ctgcggacca tttaccatga ttgctttcgc atcctgggct
    17401 ttgcttttgc cgatcaccca catattgctg gtcgttggat accccgccag accattggca
    17461 ccaccctgtg caaattgtgc agcaatagtt tcccggttct tccctgctgt taacgccagc
    17521 agtgcgccat ccgccccttt tgctggtcgg tcaagcattg gtgcaggtaa atcgtagcgt
    17581 ggcaacagag ctgctgtttg cgagttttgc tgattaaatt taagtgggta gctactctct
    17641 tgtacggcaa tagtggttgg cgctgatggg tttaccagcc atttcaactg attaaatacc
    17701 gccatgcctt gtgatacacc atatttatct tttaaagccg ttagcagtgc cagattatca
    17761 atttcgctag tgctatcaga gaagcggttt gccatggtgc ccacaaatat catcgcgaca
    17821 tcaaacggtt cccagcgctt aggagtaaag ccaaatgtat taaactgttt tggtaagagc
    17881 gtctctggat tggtatttac cttatcaatc c
//
LOCUS       DAMZET010000085        17807 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.085, whole genome shotgun sequence.
ACCESSION   DAMZET010000085 DAMZET010000000
VERSION     DAMZET010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.085"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..362
                     /locus_tag="POB34_002887"
     CDS             <1..362
                     /locus_tag="POB34_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDI5919794.1"
                     /translation="VSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFE
                     HCIANLFVIPFAIAIRHFAPTSFWQLAHSSADNFPTLTVSHFITANLLPVMLGNIIGG
                     AVLVSMCYRAIYLRQES"
     gene            complement(400..1461)
                     /locus_tag="POB34_002888"
     CDS             complement(400..1461)
                     /locus_tag="POB34_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDI5919795.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1674..3137
                     /gene="bepA"
                     /locus_tag="POB34_002889"
     CDS             1674..3137
                     /gene="bepA"
                     /locus_tag="POB34_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HDI5919796.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            3158..3517
                     /gene="arsC"
                     /locus_tag="POB34_002890"
     CDS             3158..3517
                     /gene="arsC"
                     /locus_tag="POB34_002890"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HDI5919797.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVESEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3655..4401)
                     /gene="hda"
                     /locus_tag="POB34_002891"
     CDS             complement(3655..4401)
                     /gene="hda"
                     /locus_tag="POB34_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HDI5919798.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(4451..5740)
                     /gene="uraA"
                     /locus_tag="POB34_002892"
     CDS             complement(4451..5740)
                     /gene="uraA"
                     /locus_tag="POB34_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDI5919799.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5826..6452)
                     /gene="upp"
                     /locus_tag="POB34_002893"
     CDS             complement(5826..6452)
                     /gene="upp"
                     /locus_tag="POB34_002893"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDI5919800.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6777..7814
                     /gene="purM"
                     /locus_tag="POB34_002894"
     CDS             6777..7814
                     /gene="purM"
                     /locus_tag="POB34_002894"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDI5919801.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7814..8452
                     /gene="purN"
                     /locus_tag="POB34_002895"
     CDS             7814..8452
                     /gene="purN"
                     /locus_tag="POB34_002895"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDI5919802.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8623..10689
                     /gene="ppk1"
                     /locus_tag="POB34_002896"
     CDS             8623..10689
                     /gene="ppk1"
                     /locus_tag="POB34_002896"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HDI5919803.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10694..12235
                     /gene="ppx"
                     /locus_tag="POB34_002897"
     CDS             10694..12235
                     /gene="ppx"
                     /locus_tag="POB34_002897"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HDI5919804.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12274..14517)
                     /gene="pdeF"
                     /locus_tag="POB34_002898"
     CDS             complement(12274..14517)
                     /gene="pdeF"
                     /locus_tag="POB34_002898"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HDI5919805.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIAFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTIAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(14699..14851)
                     /locus_tag="POB34_002899"
     CDS             complement(14699..14851)
                     /locus_tag="POB34_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919806.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            14869..15060
                     /locus_tag="POB34_002900"
     CDS             14869..15060
                     /locus_tag="POB34_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HDI5919807.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            15371..15889
                     /locus_tag="POB34_002901"
     CDS             15371..15889
                     /locus_tag="POB34_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDI5919808.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            15905..16444
                     /locus_tag="POB34_002902"
     CDS             15905..16444
                     /locus_tag="POB34_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HDI5919809.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQKQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16539..>17807)
                     /gene="guaA"
                     /locus_tag="POB34_002903"
     CDS             complement(16539..>17807)
                     /gene="guaA"
                     /locus_tag="POB34_002903"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDI5919810.1"
                     /translation="NEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKV
                     TAIPSDFVTVASTESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCE
                     ALWTPAKIIDDAVARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNG
                     LLRLNEAEQVLDMFGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDE
                     EALKLEDVKWLAQGTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKEL
                     FKDEVRKIGLELGLPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRK
                     ADLYDKVSQAFTVFLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGR
                     VSNRIINEVNGISRVVYDISGKPPATIEWE"
BASE COUNT         4712 a         4520 c         4392 g         4183 t
ORIGIN      
        1 ctgtcagcct cggcattatg tgcaatctga tggtttgcct ggcgctgtgg atgagttatt
       61 gcgggcgttc gttatgcgac aaaatcgtcg ccatgatttt gcccatcacc ctgtttgtcg
      121 ccagtggctt tgagcactgt atcgccaatt tgtttgtgat tccgttcgcc attgccattc
      181 gccatttcgc ccctacctcc ttctggcaac tggcgcacag tagcgcagac aattttccga
      241 cactgacggt aagccatttt attaccgcca atctgctccc ggtaatgctg ggtaatatta
      301 tcggcggtgc agtgctggtg agtatgtgtt atcgggctat ttatttacgt caggaatcct
      361 gaaaaatcag cccggcgaaa cagttcgtcg ggctgaagat tattcttgcg ctatttgccc
      421 atcgggccag gcgtggatca cggctttgat cagcgtcgcc aacggaatgg cgaagaatac
      481 gccccagaat ccccacaaac caccgaagat caccaccgat aaaataatca ccagcggatg
      541 caggttaacc gcttcggaga acaacaccgg tactagcagg ttgccgtcca gcgcctgaat
      601 aatcagatac accgcgaagc agctccagaa ttccgtgcct gcgccaaact ggaatagcgc
      661 cacgccaacc accggaatgg tcaccacaaa tgcgccaatg tacggaataa gaaccgagaa
      721 gccgaccagc accgccagca gcagcgaata gttcagccca aagagcaaga accccagcca
      781 ggtggcgatc cccaccacga tcatctccag cactttgccg cggatatagt tggtgatttg
      841 ttgattcatc tccttccaca cctgtcctgc cagtccacgg ttgcgcggca gcacccgacg
      901 aacggcgttc agcatctgct ctttgtcttt cagcaggaag aagaccatca atggcaccag
      961 caccagatag acggcaatgg tcagcaaacc gaccagcgag gcgagggaaa ttttcaccac
     1021 cgaatcgccc atggtcagca tccgactgcg catattttcg gccattgcat caataatgcc
     1081 cgcgtccatt aacgccggat agcggcgcgg caacgtggcg gcaaagtcag aaagcttatt
     1141 gagcatcccc ggcatatcgc gaattaagta gatgccctgt tgccaggcga taggcagtac
     1201 cacgaacgcc atcagtagca atataccgac aaaaaccacc aatacaatcg acgtcgccca
     1261 gcggcgggag cagccaatag attgcaggcg cacagttggc cattccagca aataggccag
     1321 cacaatagcc accagcaacg gagcaagcag gccactaaag aaaaagataa tgccaaatcc
     1381 ggcaactaaa ataaccagca aggcaatcgc ttccgggtcg ctaaaacggc ggcgatacca
     1441 ttgcatcaac atttcgagca taaagacctt ccctgaacct caagagcggg attgcgatcc
     1501 gcaattgtat cgaaatgtca caaaaaagac ttcgcttttt atgacggatt caggaaactg
     1561 aaaagtcatt tgagtgggct aatcttcgcc gttacactca aaggcggcgc ggtgggaacg
     1621 atatttcaca gtatcggtca aatgactatc ttcagaaata caggatagag gttatgttca
     1681 ggcagttgaa aaaaaacctg gttgcaaccc tcattgctgc tatgaccatt ggtcaggtag
     1741 ccccggcgtt tgccgacagc gcagacacct tgccggatat gggaacctcc gcaggaagca
     1801 cgctttccat tggtcaggaa atgcagatgg gcgactatta tgtccgccag ctacgcggca
     1861 gcgcgccgtt aattaatgac ccgctgttaa cgcaatatat taattcgctg gggatgcgtc
     1921 tggtttcgca tgccaattcg gttaagacac cgtttcattt ctttctgatc aacaacgacg
     1981 aaattaacgc ctttgctttc tttggcggca acgtggtgct gcactctgcc ctgttccgtt
     2041 attccgataa cgaaagtcaa ctggcttcag ttatggcgca cgaaatctcc cacgtcaccc
     2101 aacgtcacct ggcgcgagcg atggaagatc agcagcgcag cgcgccgctg acctgggtcg
     2161 gcgcgttagg ttctatttta ctggcgatgg ccagtccgca ggcggggatg gcggcgctga
     2221 ccggtacact ggcgggaacg cgtcagggga tgatcagttt cacccagcaa aatgaacagg
     2281 aagcggaccg cattggtatt caggtgctgc aacgctcggg attcgatccg caggcgatgc
     2341 caaccttcct cgaaaaatta ctcgatcagg cgcgttactc ctcgcgtccg ccggaaattt
     2401 tattgactca cccgttgccg gaaagtcgtc tggcagatgc ccgcaaccgt gctaatcaga
     2461 tgcgcccgat ggtggtgcag tcgtcggaag atttctatct ggcgaaagcg cgcacactgg
     2521 ggatgtataa ttccggacgt aaccagctca ccagtgattt gctggatgaa tgggcgaaag
     2581 gaaacgttcg tcagcaacga gcagcacaat atggtcgtgc tttacaggcg atggaagcca
     2641 ataaatacga cgaggcgcgt aaaacgctgc aaccgttact ggcggcagaa cctggcaacg
     2701 catggtatct cgatctggct actgatatcg atcttgggca aaacaaagcc aatgaggcga
     2761 tcaatcgtct gaaaaatgcc cgcgatttac gcaccaatcc ggtgttgcag ctcaacctgg
     2821 cgaacgctta tctgcaaggc ggtcaaccac aagaagcggc caatattctc aatcgctaca
     2881 cctttaataa taaagatgac agcaacggct gggatttgct ggcacaggcg gaagccgcgc
     2941 taaataaccg cgatcaggag ctggctgcgc gagcagaagg ttatgcgctc gccggacgac
     3001 tcgatcaggc catttcgctg ttgagtagcg ccagttcgca ggtgaaatta ggcagcctgc
     3061 aacaagcgcg ttacgatgcg cgcatcgacc agttgcgcca gctgcaggaa cgctttaagc
     3121 cttataccaa gatgtaattc gcattaaagg aagaacaatg accaaacagg taaaaatcta
     3181 ccataaccca cgctgttcaa agagccggga aacgctgaac ctgctgaaag aaaacggcgt
     3241 ggagtcagaa gtggtgctct accttgagac acccgctgat gcggcaacgc tgcgcgattt
     3301 gctgaaaatg ctggggatga atagcgcccg tgaattgatg cgccagaaag aggatcttta
     3361 taaagaactg aacctggctg acagttcgct tagcgaagag gcgctgattc aggcaatggt
     3421 ggataatccg aagctaatgg agcgcccgat tgtggtcgcg aatggcaaag cgcggattgg
     3481 tcgtccaccg gagcaggtac tggaaatcgt gggttaagat gtgctccatc gctagttgaa
     3541 gcacattgcc ggatgcgacg cttgccacgt cttatccggc ctacgaatac tatggattaa
     3601 cttcgcgtag ttcggataag gcgttcgcgc cgcatccgac aataaacacc ttatctacaa
     3661 cttcagaatt tctttcacaa acggaatggt cagcttacgt tgcgcggtaa tcgacgcacg
     3721 atccaactga tccaacgtca taaatagcgt gcgcatttct ctgtcgagcc gcttcagcaa
     3781 gaaacgcccc acatcttccg gcagttcaaa accacgcaaa cgcgcgcgta actgtagcgc
     3841 ctgcaactta tcttcatcag aaagtggctg caatttgtag atctgccccc agtcgagtcg
     3901 cgacgcgaga tccggtaatc ccagattcaa ctgccgcggt ggacgatcgc cggtgatcaa
     3961 caatcgtgtt ttgcccgatt ccagaattcg attgtagaga tcgaaaatcg ccatctccca
     4021 caactcatcg cctgcaatac actcaatgtt gtcgatacag accagcgaca aatgctccat
     4081 accgtcgagc acttccggaa caaaccaggt gcgtttatcc agcgggacat agcccaccgc
     4141 atcgccacgc tgcgacaatt ccgcgcaagc cgcgtgcagc agatggctgc gccccgcgcc
     4201 ttcgcgtgcc cagagataga tgtaaccgct atgttcctga cgcagcacgt tttgcagcgc
     4261 agccagtaaa gaggagttat cccccggcca gaaacttgca aaggtttcgt cgtcaggaag
     4321 ataaagtggc aaagagagct gtgccggtgt gttcagagat acctcaacca ggatttcaca
     4381 aaatcgcgag aagtttacca cagaatccca tgatgtttga accgggcagc aacactgccc
     4441 ggtcggtaca ttatttgtct gttatatccg cgtcttctgc gtccagcacc acttcttcag
     4501 ggcgcagcac actgatcagt ttgaaaatca aacttaagcc gataccgacg atggtcgcca
     4561 gcgccatacc ttttaactcc gccgcgccga tgtttacctt cgcgccactg acgccgatga
     4621 tcaaaatcac ggaagtcagg atcaggttct gtgctttgtt gtaatccact ttcgattcga
     4681 tcaaaacacg aataccggaa gcaccgatga caccataaag cagcagcgaa acgccgccca
     4741 tcaccggcaa tgggatcatc tggatagcgg cagccagttt accgacgcag gaaagcagga
     4801 tagcgaaaat cgccgccccg ccgataaccc aggtactgta aacacgggtg atcgccatca
     4861 cgccaatgtt ttctccgtaa gtggtatttg gcgtagagcc aaagaagccg gaaatcacgg
     4921 tcgacaagcc attagcaaac atcgaacggt gcagacctgg atcgcgcagc agatcttttt
     4981 tgacgatatt agccgttact accaggtgcc ctacgtgttc ggcaataacc actaacgccg
     5041 ctggcagaat agtcagaatg gcaaaccact cgaagcgcgg cgtatagagg gttggcagcg
     5101 caaaccagtg agcattaata atcggcgtgg tatcgacaat tcccattgcg aaagagagcg
     5161 cgtaccccac cagcacgcca attaaaatcg ggataattgc caggaaacca cgaaacagca
     5221 cggaacctaa aaccgtgacc gccagggtgg taatagagat gatgatggtt ttggagtctg
     5281 gcgtttgccc ttcagccggg agtaaacccg ccataccggc agctacgccc gccagctcca
     5341 gaccgatgac ggcaacgatt gcgcccattg ccgcaggtgg aaacagcacg tccagccagc
     5401 cggtccccgc tttcttcacg ataaaagaaa ccaggcagaa cagcacgccg cacataataa
     5461 agccgcccag cgcgacttca taccctaacg gcaacagtaa caataccggt gaaataaagg
     5521 caaagctgga accaagataa gccggaattt tccctttaca gatgaagaga tacagcagcg
     5581 ttccaatacc gttaaataac agtacagtcg ccgggttaat atgaaataag acgggcacca
     5641 ggacggttgc accaaacatg gcgaacaaat gttgcaaact aagcgggatt gtctgtaaaa
     5701 gtggcggtct ttcactcacc ccgatagcac ggcgcgtcat agtattatcc tctgtattat
     5761 gtgttatagg cgctttactc aaaaaaaagc cgactcttaa agtcggcttt aattattttt
     5821 attctttatt tcgtaccaaa gattttgtca ccggcatcgc cgaggcccgg aataatgtat
     5881 ccgtgctcgt tcagtccctg atcaatcgat gcggtataca gttcgacgtc cgggtgcgct
     5941 ttttccagcg cagcgatacc ttctggcgca gcgaccagca ccagaacttt gatgctgctg
     6001 cagcccgctt ttttcagcag gtcgatggtc gcgataacgg aaccaccggt tgccagcatt
     6061 gggtcaacga tcagcgccat acgctcatcg atgttagaaa ccagtttctg gaagtacggt
     6121 accggctcca gcgtttcttc attacggtac ataccgacaa cgctgatgcg cgcgctcgga
     6181 acgttttcca gcacaccgtc catcatacca agacccgcac gcagaattgg cacaacggta
     6241 attttcttac ctttgatctg gtcgatttct accgggccgt tccagccttc gatagttact
     6301 ttttccgttt cgaggtcggc ggtcgcttcg taagtcagca ggctacccac ttcggaagcg
     6361 agttcgcgaa agcgcttggt gctgatatct tgctcacgca tcagtcccag cttgtgtttg
     6421 acgagtgggt gtttgacttc cacgatcttc atactctttc tcctttgagg ggcagccaca
     6481 aaaaaaatcg acggattata cctcctttct tcaaggcggc aatattcttt tcgttgactt
     6541 tagtcaaaat gataacgatt tgagataaag ttattttata ttcaaatggt tatgaaagaa
     6601 gattattcca tccgaaaact aacctttacc ctggcacaag tcttctttcg ccgcgtgcct
     6661 gggcaaaaga cgtgcaaaaa ggttgtgtaa agcagtctcg caaacgtttg ctttccctgt
     6721 tagaattgcg ccgaatttta tttttctacc gcaagtaacg cgtggggacc caagcagtga
     6781 ccgataaaac ctctcttagc tacaaagatg ccggtgttga tattgacgcg ggtaatgctc
     6841 tggttggaag aatcaaaggc gtagtgaaga aaacgcgtcg tccggaagtg atgggcggtc
     6901 tgggcggctt cggtgcgctg tgtgcattgc cgcaaaaata tcgtgaaccc gtgctggttt
     6961 ccggcactga cggcgtaggt accaagctgc gtctggcaat ggacttaaaa cgtcacgaca
     7021 ccattggtat tgatctggtc gccatgtgcg ttaatgacct ggtggtgcaa ggtgcagagc
     7081 cgctgttttt cctcgactat tacgcaaccg gaaaactgga tgttgatacc gcttcagcgg
     7141 tgatcagcgg tatcgcggaa ggatgtctgc aatcgggctg ttcactggtg ggtggcgaaa
     7201 cggcagaaat gccggggatg tatcacggtg aggattacga tgtcgcgggt ttctgcgttg
     7261 gcgtggtaga aaaatcagaa atcatcgacg gctctaaagt cagcgacggc gatgtgctga
     7321 ttgcactcgg ttccagcggt ccgcactcga acggctattc gctggtgcgc aaaattcttg
     7381 aagtcagcgg ttgtgatccg caaaccaccg aacttgatgg taagccatta gccgatcatc
     7441 tgctggcacc gacccgcatt tacgtgaagt cagtgctgga gttgattgaa aaggtcgatg
     7501 tgcatgccat tgcgcacctg accggcggcg gcttctggga aaacattcca cgcgtattgc
     7561 cagataatac tcaggcagtg attgatgaat cttcctggca gtggccggaa gtgttcaact
     7621 ggctgcaaac ggcaggtaac gttgagcgcc atgaaatgta tcgcaccttc aactgcggcg
     7681 tcgggatgat tatcgccctg cctgctccgg aagtggacaa agccctcgcc ctgctaaacg
     7741 ccaacggtga aaacgcgtgg aaaatcggta tcatcaaagc ctctgattcc gaacaacgcg
     7801 tggttatcga ataatgaata ttgtggtgct tatttccggc aacggaagta atttacaggc
     7861 aattattgac gcctgtaaaa ctaacaaaat taaaggcacc gtacgggcag ttttcagcaa
     7921 taaggccgac gcgttcggcc ttgaacgcgc ccgccaggcg ggtattgcaa cgcatacgct
     7981 catcgccagc gcgtttgaca gtcgtgaagc ctatgaccgg gagttgattc atgaaatcga
     8041 catgtacgca cccgatgtgg tcgtgctggc tggttttatg cgcattctca gcccggcgtt
     8101 tgtctcccac tatgccgggc gtttgctgaa cattcaccct tctctgctgc cgaaatatcc
     8161 cggattacac acccatcgtc aggcgctgga aaatggtgat gaagagcacg gtacatcggt
     8221 gcatttcgtc accgatgaac tggacggtgg cccggttatt ttacaggcga aagtcccggt
     8281 atttgctggt gatacggaag atgacatcac cgcccgcgtg caaacccagg aacacgctat
     8341 ttatccactg gtgattagct ggtttgccga tggtcgtctg aaaatgcacg aaaacgccgc
     8401 gtggctggat ggtcaacgtc tgccgccgca gggctacgct gccgacgagt aatgccccgt
     8461 agttaaagcg ccagctctgc cgctggcgtt tttcaattca cctgtaaatc gcaagctcca
     8521 gcagtttttt tccccctttt ctggcatagt tggacatctg ccaatattgc tcgccataat
     8581 atccaggcag tgtcccgtga ataaaacgga gtaaaagtgg taatgggtca ggaaaagcta
     8641 tacatcgaaa aagagctcag ttggttatcg ttcaatgaac gcgtgcttca ggaagcggcg
     8701 gacaaatcta acccgctgat tgaaaggatg cgtttcctgg ggatctattc caataacctt
     8761 gatgagttct ataaagtccg cttcgctgaa ctgaagcgac gcatcattat tagcgaagaa
     8821 caaggctcca actctcattc ccgccattta ctgggcaaaa ttcagtcccg ggtgctgaaa
     8881 gccgatcagg aattcgacgg cctctacaac gagctgctgc tggagatggc gcgtaaccag
     8941 atcttcctga ttaatgaacg ccagctctcc gtcaatcaac aaaactggct tcgtcattat
     9001 tttaagcagt atctgcgtca gcacattacg ccgattttaa tcaatcctga cactgactta
     9061 gtgcagttcc tgaaagatga ttacacctat ctggcggtgg aaattatccg tggcgatacc
     9121 atccgttacg cgctgctgga gatcccatca gataaagtgc cgcgctttgt gaatttaccg
     9181 ccagaagcac cgcgtcgacg caagccgatg attcttctgg ataacattct gcgttactgc
     9241 cttgatgata ttttcaaagg cttctttgat tatgacgcgc tgaatgccta ttcaatgaag
     9301 atgacccgcg atgccgaata cgatttagtg catgagatgg aagccagcct gatggagttg
     9361 atgtcttcca gtctcaagca gcgtttaact gctgagccgg tgcgttttgt ttatcagcgc
     9421 gatatgccca atgcgctggt tgaagtgtta cgcgaaaaac tgactatttc ccgctacgac
     9481 tccatcgtcc ccggcggtcg ttatcataat tttaaagact ttattaattt ccccaatgtc
     9541 ggcaaagcca atctggtgaa caaaccactg ccgcgtttac gccacatttg gtttgataaa
     9601 gcccagttcc gcaatggttt tgatgccatt cgcgaacgcg atgtgttgct ctattatcct
     9661 tatcacacct ttgagcatgt gctggaactg ctgcgtcagg cttcgttcga cccgagcgta
     9721 ctggcgatta aaattaacat ttaccgcgtg gcgaaagatt cacgcatcat cgactcgatg
     9781 atccacgccg cacataacgg taagaaagtc accgtggtgg ttgagttaca ggcgcgtttc
     9841 gacgaagaag ctaacattca ctgggcgaag cgcctgaccg aagcaggcgt gcacgttatc
     9901 ttctctgcgc cggggctgaa aattcacgcc aaactgttcc tgatttcacg aaaagaaaac
     9961 ggtgaagtgg tgcgttacgc acacatcggg accgggaact ttaacgaaaa aaccgcgcgt
    10021 ctttatactg actattcatt gctgaccgcc gatgcgcgta tcaccaacga agtacggcgg
    10081 gtatttaact ttattgaaaa cccataccgc ccggtgacat ttgattattt aatggtgtca
    10141 ccgcaaaact ctcgccgtct gttatatgaa atggtagacc gcgaaatcgc caacgcgcag
    10201 caagggctgc ccagtggtat caccctgaag ctaaataacc ttgtcgataa aggcctggtt
    10261 gatcgtctgt atgcggcctc cagctccggc gtaccggtta atctgctggt tcgcggaatg
    10321 tgttcgctga tccccaatct ggaaggcatt agcgacaaca ttcgtgccat cagtattgtt
    10381 gaccgttacc tagaacatga ccgggtttat atttttgaaa atggcggcga taaaaaggtc
    10441 tacctttctt ccgccgactg gatgacgcgc aatattgatt atcgtattga agtggcgaca
    10501 ccgctgctcg atccgcgcct gaagcagcgg gtgctggaca tcatcgacat attgttcagc
    10561 gatacggtca aagctcgtta tatcgataaa gaactcagta atcgctacgt tccccgcggc
    10621 aatcgccgca aagtacgggc gcagttggcg atttatgact acatcaaatc actcgaacaa
    10681 cctgaataac cctatgccaa tacacgataa atcccctcgt ccgcaggagt ttgctgcggt
    10741 cgatcttggt tcaaacagtt ttcacatggt catagcccgt gtggtagatg gtgccatgca
    10801 gattattggc cgcctgaaac agcgggtgca tctggcggac ggcctggggc cagataatat
    10861 gttgagtgaa gaggcaatga cgcgcggttt gaactgtctg tcgctgtttg ccgaacggct
    10921 acaagggttt tctcctgcca gcgtctgtat agtcggcacc catacgctgc gtcaggcgct
    10981 gaacgccact gactttctga aacgcgcgga aaaggtcatt ccctacccga ttgaaattat
    11041 ttccggtaat gaagaagccc gtctgatttt tatgggcgtg gaacataccc aaccggagaa
    11101 aggccgcaaa ctggttattg atattggcgg cggatctacg gaactggtga ttggtgaaaa
    11161 tttcgaaccg attctcgttg aaagccgccg gatgggttgt gtcagctttg cccagcttta
    11221 cttccctggc ggggtcatca ataaagagaa ttttcagcgc gctcgtatgg cggcagcaca
    11281 aaaactggaa actttaacct ggcaattccg tattcagggc tggaacgtgg caatgggcgc
    11341 ttccggtacc ataaaagccg cccatgaagt gttaatggaa atgggcgaga aagacgggat
    11401 aattaccccg gaacgtctgg aaaaactggt aaaagaagtt ttacgtcacc gtaatttcgc
    11461 atcgctgagt ttaccgggtc tttccgaaga gcggaaaaca gtcttcgttc ctggactggc
    11521 gattttatgc ggtgtgtttg atgctttagc catccgtgaa ctgcgccttt ctgacggggc
    11581 tttacgcgaa ggcgtactgt atgaaatgga aggacgtttc cgtcatcagg atgtgcgtag
    11641 tcgcaccgcc agcagcctcg ccaaccagta tcacatcgac agcgagcagg cccgacgggt
    11701 gctggatacc actatgcaaa tgtacgaaca gtggcgggaa cagcaaccga agctggcgca
    11761 tccgcaactg gaggcgctac tgcgatgggc cgccatgctg catgaggtcg ggttgaatat
    11821 caaccacagc ggtttgcatc gccactccgc ttatattctg caaaacagtg acttgccggg
    11881 ttttaatcag gaacagcagc tgatgatggc gacactggtg cgctatcacc gtaaagcgat
    11941 taagctcgac gatctgccgc gctttacctt gtttaagaag aaacagttcc tgccactgat
    12001 acagctattg cgccttggcg tattactaaa caatcaacgt caggcaacca ccacaccgcc
    12061 aacattgaca ctgattactg atgacagtca ctggacactg cgtttcccgc atgactggtt
    12121 tagtcagaat gcgctggtac tgcttgatct ggaaaaggag caagaatact gggaaggcgt
    12181 ggctggctgg cggttgaaaa ttgaagaaga aagtacacct gaaatcgccg cttaatgctt
    12241 tgcgggccga cgagaaatgt cggcccgcat tattcaggca ctttcgcgaa tgggttcgat
    12301 ttcattcagc gtatcaatta acggttgcgg cttaccaata agataaccct gcatataatc
    12361 gatccccaaa gagagcaccg cctcgcggat ctcttcgttt tcaacgtact ctgccactac
    12421 ccgcattttc ttcattcgcg ccaggtggca aatcgatgcc actatctgat aatccagact
    12481 atttgacaca atattgcgga taaaactgcc gtcaatttta agcagatcgg cattcacatt
    12541 tttaagccgc gcatagctgg cgtagccggt gccgaaatca tcaatcgcaa tctggcagcc
    12601 taattcctga agatgctgca aggtgatttg cgcctgctta acattggtca gagcattact
    12661 ttcggtgact tcaaaaataa gttgccacgc ttcaatctga tatttatcca gcaactgact
    12721 gacttcaaca ggaaaacgag cctgacatac cgaggttgga gacagattaa tagcaaaacg
    12781 gtgagcgggc atcttcgctc tgttttcagc cataaattgc agcgtatgct caatgaccca
    12841 catgtcgata ctcgacgata aaccaaattc gtgcgcgacc ggcaagaagc tatcggggcc
    12901 gatcagttca tcattctcac ctttcatgcg cagaagaatt tcatggtaaa catcaccacg
    12961 cataccggta atcggctggg ccatcaggaa aaaatggttg tgttccagcg cctgctgtag
    13021 tcgattcatc atcgcgactt tatctttcaa ttcgcgttgc aaatacattg ccccgcgacg
    13081 ctgcatattt tccggggcgt tggtcacgat ggaaagttcg gcgatcgtat ttagctctcc
    13141 cagcagcagg tagatatgat tcactggcga gcgcacatag cagtaactga cgccaatctg
    13201 cggttgcatc ggcatgccat cccaaaagaa acgaaattgc ttgagatggc tatccagtgc
    13261 ggtaatgcgc tcctggtgcg attctgtatt cagccgcagc gcgagatcgt tacccgaaag
    13321 ctgataaaca tcttcacccg gttccagcaa gggtgacagc cagtgagaaa gtttttgctt
    13381 gtattgaatc cgcagcatga tgccatagtt cttaaccagc atttccatgc cagggatgcg
    13441 taaataacaa agcgcagacc agggggcatc acgcaacgcg cgattcaggg cgcgaacatt
    13501 tggcagatga accaccgggt ccacatacgc aagccgctga atgcgtctga ctaccgctcg
    13561 ctgacgagtt gccagtacag ccatataatt gacaataaaa gagaatacca gataactgga
    13621 ggaggttatg gtcagctgcg tggtatagcc aggataaatg ggaatgtaat tttgatagct
    13681 gtggatgctg atcatcaaca cgaccgccca gagcagcgaa atcagcttat aaccatagcg
    13741 catcgctccc cacatcatca ggggcagcaa taatgacaag gtataattgg tgctaaaaat
    13801 tgtgcttttt tcatttaacg gcatgcataa cagcaacaat aaagcaccta atgccagtag
    13861 ccagagcgcg aactcttttt tggtgacttt ggcatcaacc tgctgtttta attgcgaata
    13921 atagctacgt aaataaaatg gatttcgcac tacccgaatg atgaagtagc acagcgggac
    13981 accgatcaga ttacccacca gtaaggcctg ataattgatt aaagtcccga ggttaaaagg
    14041 catgacaccg accagatttt ctctgctcgc cagcaatcct acaaatgcag caaactggaa
    14101 aagtatcaga aacagcgttg caggaaacgc aatctgccag aatatacgct gggaaattaa
    14161 acgggtatca ccatgtgaaa cgttgttacg ccggggagta aagaccctgt aaccgcccca
    14221 acagagaatg ataatgataa taaaattagc cgttaatgag agcgtttcgt aaaaacccag
    14281 ctctgcatat ttgcggacaa aaatccctaa ggtgatccct ggcaatgccg cccagctgaa
    14341 aaacatcatc atgctgatca tcagtgccaa aggcagataa aagagaaaaa cctcaccgga
    14401 agaaatatgc gcgaaagtat taatgtgggc gaaaatgggc aagattaaag aaggcaggaa
    14461 cagcggaagc ccccaccatt tatcacgtat ttttatataa gttgcattca gtttcataga
    14521 tgctcagcag aatcccccac atcctgaagg aggtgtattc agacaggcat cccacctgac
    14581 ttcgaatgat gattattcat cactatagag agcattgatt ctaagtgtca tatgaaagtt
    14641 ccaattgata tatatcaaac aaaataaccc tgattaatga attattacgt ttatcatgtt
    14701 aattcatcat tattacatca tcattgtaaa taattaaatt aacttccata acattaaaat
    14761 atgtatccac tgacgctttt ttacataacg aagaattgac cattttgtcc tgttgtgcct
    14821 taatgtaagt accgtccaca gcgtgggaca tacttcaagg aaccttttgt gagtcaggca
    14881 accagtatgc gaaaacgaca ccgatttaac agtcgcatga cccgtatcgt actgctcatc
    14941 agctttatct tcttctttgg ccgttttatc tactcgtccg tcggtgcctg gcagcaccat
    15001 cagagcaaaa aagaagctca gcaatccaca ctctccgtcg aatcaccggt acaacgttag
    15061 cggttacctt ctccactttc acagaacata acggcacttc gctgtcggat gcttttgctg
    15121 tttgggatta tcaaagcggc agatattctt tcatcttaaa ttttacgtct ttgtcctgac
    15181 tgatgtttat cctgtttggc tgcgaaataa atataaaatt aatatatatg ttgtaatgat
    15241 atatttttat aaattattcc ctgcgtgaat tttaataaat ttaatctatc cctttatacg
    15301 caatacattt actttcctct tttgatgatc ttaaatgtct tatttttcgt aatgtgtata
    15361 acaaggaata gtgatgaaat ttaaaaaatg tcttctgcct gtggcaatgt tagcgtcatt
    15421 cactctggca ggatgccagt caaatgctga cgatcatgcc gccgatgttt atcaaaccga
    15481 tcaactgaat accaaacaag aaactaaaac cgttaatatt atttccattc ttcccgcaaa
    15541 agttgccgta gacaactccc aaaataaacg gaacgcacaa gccttcggcg cgcttattgg
    15601 cgcagtcgct ggcggtgtta tcggccacaa cgtcggttct ggcagcaatt ccggaacgac
    15661 ggcaggggca gttggcggcg gagctgtagg cgcggcagcg ggttctatgg tgaatgataa
    15721 aaccttagtg gaaggtgttt ctttaacgta taaggaaggc accaaagtgt atacctccac
    15781 ccaggtgggt aaagagtgcc agtttacgac aggtttagcc gttgttatta ccacgacgta
    15841 taacgaaacg cgtattcagc caaataccaa atgtcctgaa aagagctaat aatcaggagg
    15901 agtcatgaag aaagtttttc tttgcgccat cttggcctcc ttaagctatc cggctatcgc
    15961 ctcatcattg caggatcaac tctcggctgt cgcagaagcg gaacagcaag gtaaaaatga
    16021 agagcaaaag cagcatgacg aatgggtcgc ggagcgcaac agggaaatcc agcaagagaa
    16081 gcaacgtcgc gcaaatgccc aggccgccgc taacaaaaga gcggcaacgg cagcagcaaa
    16141 taagaaagct cgtcaggata aactggacgc cgaagcctct gcggacaaaa aacgcgatca
    16201 aagttatgaa gatgagctac gcagcttaga gattcagaaa caaaaactgg cgctggcgaa
    16261 agaagaagcc cgcgttaagc gagaaaacga atttatcgat caggaactga agcacaaagc
    16321 tgcgcaaacc gatgtggtgc aatctgaagc tgacgccaac agaaatatga ctgaaggcgg
    16381 tcgcgatctg atgaaaagcg tgggcaaagc agaagagaac aaatcggaca gctggtttaa
    16441 ttaatcgatg ttagtaactt caagcctatg attcttgaag ataaaaaacc ctctgtagta
    16501 acagagggtt ttgttcattc atagtgcagg gatcaaaatc attcccactc aatggtagct
    16561 ggcggcttgc cgctgatgtc atacaccacg cgggaaatac cgttcacttc attgataatg
    16621 cggttggaaa cgcgaccgag gaaatcgtac ggcagatgcg cccagtgtgc ggtcataaag
    16681 tcgatggttt cgacagcacg cagagagaca acccagtcat acttacgacc atcgcccatt
    16741 acgccaacgg aacgtaccgg caggaacaca gtgaacgcct ggctgacttt gtcgtacagg
    16801 tccgctttac gcagttcttc aatgaagatg gcgtcagcac ggcgcagcag gtcacagtac
    16861 tctttcttca cttcacccag cacacgaacg ccaaggcctg gtcccgggaa cgggtgacgg
    16921 tacagcatgt cgtacggcag gcccagctcc agaccaatct tacgcacttc gtctttgaac
    16981 agctctttca gcggttcaac caggcccatc ttcatctctt tcggcaggcc gcccacgttg
    17041 tggtgagatt tgatgacgtg tgctttaccg gttgcagaag ccgcagattc gataacgtca
    17101 gggtagatgg tgccctgcgc cagccacttc acgtcttcca gtttcagcgc ttcttcatcg
    17161 aatacttcaa cgaaaacgcg cccgatgatt ttacgttttg cttccggatc gttttcgcca
    17221 gccagcgctg acaggaagcg atcttctgcc ggtacgtgaa caatgttaag accaaagtga
    17281 tcgccaaaca tatccagaac ctgctctgct tcgttgagac gcagcaggcc gttgtcgacg
    17341 aatacgcaag tcaggttttt accgatagcg cggtgcagca gcattgcggt tacggaggaa
    17401 tccacaccac cagagaggcc gaggatgact ttatcgtcgc ctacctgctc gcggatgcga
    17461 gctacagcat cgtcgataat tttcgctggc gtccacaggg cttcacactg gcagatatca
    17521 cgcacaaaac gctccagcat gcgcataccc tggcgggtgt gagtcacttc cgggtggaac
    17581 tgtacgccat agaagcgttt ttcttcgtta gccataatgg caaacgggca gctttcggtg
    17641 ctggctacgg tgacgaagtc ggacgggata gcggtaactt tatcgccgtg gctcatccag
    17701 acatcgagca gcggtttacc gtctgcggtc agcgcatctt cgataccgcg aaccagtgcg
    17761 ctgtcgttta cgacttcaac ctgcgcgtag ccaaattcac gttcgtt
//
LOCUS       DAMZET010000086        17779 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.086, whole genome shotgun sequence.
ACCESSION   DAMZET010000086 DAMZET010000000
VERSION     DAMZET010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.086"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            148..957
                     /gene="mutM"
                     /locus_tag="POB34_002904"
     CDS             148..957
                     /gene="mutM"
                     /locus_tag="POB34_002904"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDI5919811.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(996..1475)
                     /gene="coaD"
                     /locus_tag="POB34_002905"
     CDS             complement(996..1475)
                     /gene="coaD"
                     /locus_tag="POB34_002905"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDI5919812.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1483..2760)
                     /gene="waaA"
                     /locus_tag="POB34_002906"
     CDS             complement(1483..2760)
                     /gene="waaA"
                     /locus_tag="POB34_002906"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HDI5919813.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            3173..4231
                     /gene="rfaQ"
                     /locus_tag="POB34_002907"
     CDS             3173..4231
                     /gene="rfaQ"
                     /locus_tag="POB34_002907"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HDI5919814.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            4228..5352
                     /locus_tag="POB34_002908"
     CDS             4228..5352
                     /locus_tag="POB34_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDI5919815.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPPNSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            5345..6142
                     /gene="rfaP"
                     /locus_tag="POB34_002909"
     CDS             5345..6142
                     /gene="rfaP"
                     /locus_tag="POB34_002909"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HDI5919816.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            6158..7174
                     /gene="waaO"
                     /locus_tag="POB34_002910"
                     /gene_synonym="rfaI"
     CDS             6158..7174
                     /gene="waaO"
                     /locus_tag="POB34_002910"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HDI5919817.1"
                     /translation="MSAHYFNPQEMITKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKVLYLDADIACQGNLKPLITMDLANNIAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSARPFIKAKEASPWKNEPLMRPVNSNYARYCAKHNFKQNKPINGIMNYIYYFYL
                     KIIK"
     gene            7191..8186
                     /locus_tag="POB34_002911"
     CDS             7191..8186
                     /locus_tag="POB34_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HDI5919818.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNDSTQEAC
                     AKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLLKLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLGKDFDTIYTLKNELHDRSHRKFQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            8196..8888
                     /gene="rfaY"
                     /locus_tag="POB34_002912"
     CDS             8196..8888
                     /gene="rfaY"
                     /locus_tag="POB34_002912"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HDI5919819.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLRNIDD
                     TKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            8914..9939
                     /locus_tag="POB34_002913"
     CDS             8914..9939
                     /locus_tag="POB34_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HDI5919820.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVIIYKVSNAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDEDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            10024..11007
                     /locus_tag="POB34_002914"
     CDS             10024..11007
                     /locus_tag="POB34_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDI5919821.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMHSRQHETGGDAIYCGLYGHHIKNGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTISHYRHESFAHEKIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFK
                     QLNQLVESNEKELKDCNLPGKRDKRRAKILASKNYIIWRMVRLVNRKKNKR"
     gene            11053..12306
                     /locus_tag="POB34_002915"
     CDS             11053..12306
                     /locus_tag="POB34_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDI5919822.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLVTPKKTITNNPTLLIFISLCLLGIVNIIWYSHYKISGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDLRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALILIVMDSYINKRINYKLYCFITIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPYVDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFITIILLCAYFKAQSDQCLLEK"
     gene            complement(12376..13347)
                     /gene="rfaC"
                     /locus_tag="POB34_002916"
     CDS             complement(12376..13347)
                     /gene="rfaC"
                     /locus_tag="POB34_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HDI5919823.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAAMI"
     gene            complement(13351..14397)
                     /gene="rfaF"
                     /locus_tag="POB34_002917"
     CDS             complement(13351..14397)
                     /gene="rfaF"
                     /locus_tag="POB34_002917"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HDI5919824.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALFGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(14407..15339)
                     /gene="rfaD"
                     /locus_tag="POB34_002918"
     CDS             complement(14407..15339)
                     /gene="rfaD"
                     /locus_tag="POB34_002918"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HDI5919825.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNKGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVMEYMAWLNRDA"
     gene            15553..16749
                     /gene="kbl"
                     /locus_tag="POB34_002919"
     CDS             15553..16749
                     /gene="kbl"
                     /locus_tag="POB34_002919"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDI5919826.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            16759..>17779
                     /gene="tdh"
                     /locus_tag="POB34_002920"
     CDS             16759..>17779
                     /gene="tdh"
                     /locus_tag="POB34_002920"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDI5919827.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSW"
BASE COUNT         4794 a         3864 c         4200 g         4921 t
ORIGIN      
        1 atccagttgt tcgtcagcac gtgatctaca aagaagcgaa aatcaaataa ttctcgcttt
       61 gatgtaacaa aaaacctcgc tccggcgggg ttttttgtta tctgcttgcc cccatattga
      121 ttgcatctgt tcattcctgg agatgctatg cctgaattac ccgaagttga aaccagccgt
      181 cgcggcatag aaccgcatct cgttggtgca accattcttc atgcagtggt gcgcaacgga
      241 cgcttgcgct ggccggtttc agaagagatc taccgtttaa gcgaccaacc agtgcttagc
      301 gtgcagcggc gggctaaata tttgctgctg gagctgcctg agggctggat tatcattcat
      361 ttggggatgt ctggcagcct gcgcatcctt ccagaagaac ttccccctga aaagcatgac
      421 catgtggatt tggtgatgag caacggtaaa gtgctgcgct acaccgatcc gcgccgcttt
      481 ggtgcctggc tgtggaccaa agagctggaa gggcataatg tgctggccca tcttggaccg
      541 gagccgctca gcgacgattt caatggtgag tatctgcatc agaagtgcgc gaagaaaaaa
      601 acggcgatta aaccgtggct gatggataac aagctggtgg taggggtagg gaatatctat
      661 gccagcgaat cactgtttgc ggcggggatc catccggatc ggctggcgtc atcactgtcg
      721 ctggcagagt gtgaattgtt agctcgggtg attaaagcgg tgttgctgcg ttcgattgag
      781 cagggtggta caacgctgaa agattttctg caaagtgatg gtaaaccggg ctatttcgcg
      841 caggaattgc aggtttacgg gcgaaaaggc gagccgtgtc gggtgtgcgg tacgccgatt
      901 gtggcgacta aacatgcgca gcgggcaacg ttttattgtc ggcagtgcca gaagtaattc
      961 atgcgcgccg gatggcatac catccggcat aaacgctacg ctaacttcgc catcagcgcc
     1021 tgatggacat tctccggcag gaaatgggtg acatcgccct gatggcgcgc cacctctttc
     1081 accaacgatg aagagataaa cgaccactct ttcgacggca tcagaaacac actttccagt
     1141 tccggcatta agtggcgatt catatgcgcc agctgcattt catattcaaa atccgccacc
     1201 gcacgcaggc cacgaatcag caccgtagcg tgttgattac gggcgaagtt cgccatcaaa
     1261 tcactaaacc cgaccacttc cacgttcccc agatgcgcgg ttgcctgctg tgccagcgcc
     1321 acacgctctt ccagggtaaa catcggtttt ttactggggc tggcggcaat cgccagaata
     1381 acgtgatcga acatttgcgt ggcgcgcgtc acgatatcga tatgaccatt ggtaatggga
     1441 tcgaaagtac caggataaat cgcccgtttt tgcataacaa cctcaatgcg ttttcggtgg
     1501 caggtaaggt tccagcagtt gaagcagacg ctgtagcgcg ccctggtttt gatacagtac
     1561 ttcaacggca tgacggccat agaaactacg gtaatcggcg tcggtgagta aagaggacac
     1621 ctcttttgca agcgtagtgg catcggtaac ggtaatcagc ccgcttgcct gctccagccg
     1681 cgcgcaaatg tctttaaagt taaaagtatg cggccccatc aataccggaa tagcgtgtgc
     1741 ggcagcttcc agcggattat gcccaccacg ttcaaccagt gaaccgccaa caaaggcgag
     1801 atcggcaatg ccgtacagta acatcaactc gcccatcgta tcgccaacca caacctgcgt
     1861 gctggtggag gggacttccc ctgaagagcg tgtgatatag cttagtccag cctggcggac
     1921 aagattaatc gcatccggga agcgttctgg atgacggggt accaggatga gcaataaatt
     1981 cgggaattgc tgtaacaatg cctgatgtgc ggcgatcacc acactctctt cgccttcgtg
     2041 agtgctggtg gcaatccata ccgggcggtg tggtgcccac tggcggcgca gcgtcacggc
     2101 tttagcagcc aactgcggcg ttacagaaat atcgaatttc aggctaccgg taacggtcac
     2161 ctgattattt tttgcgccca gcgccacaaa acgtgcacca tcttcttcat tttgcgcagc
     2221 aatcagcgta atacgacgca gcaagcgacg gacgaattta cccagtttgg cataacctgc
     2281 ggccgagcgg gcagagagtc gcgcgttagc gatcaccagc ggaattttac gtttgtgtag
     2341 cgccgcaatc aggttaggcc atagttcggt ttccataatc aacaccagtt tggggtcgac
     2401 tttattcagg aaacggttga gcgcatcggg caggtcatac ggcagataaa cgtgctgaac
     2461 atccttcccg aaagccgatt gtacgcgctc cgaaccggtt ggcgtcatgg ttgtaacggt
     2521 aatcggtaaa tcaggataac gatggcgcag cgcgcgcacc aacgggatcg ccgccagagt
     2581 ttcaccgacg gagacggagt gcagcataat gccgcctggt tttagcggat ggcggtaaaa
     2641 accgtaacgt tcaccccagc gttttcgata ggccggagcc ttacgtccgc gcacccagag
     2701 ccgtatccag atcagcggct gaataaggta gagaagggcg gtgtaaagca attcgagcat
     2761 agtaaatagc tgacttatgg atgtgctggg gattctatgt atttagctgt ggctttacca
     2821 ttacttttcc cgtttttgac ttaaatagca atttacccgc caattcttgc tgaacatcta
     2881 tacttttttt gtatttttat gcgattcatt gatttctgct gtacgtgcag gaaggaatcg
     2941 cagagcaaca gtgaaagaat atggttgcta atgattttat ttgattgatt tctttattga
     3001 aaatcaacaa tgtgcttaaa aatcattcgg gtcggattgc cgcgaaagaa gtgatacact
     3061 agcgcgtcaa tatcagtgcg gtatcagtat tcaggtagct gttgagcctg gggcggtagc
     3121 gtgctttttc tgcttcacct taatcggata atctcaacaa aaagagttac ttgtggataa
     3181 gccatttcga agaattttgc tcattaagat gcgttttcat ggggatatgt tattaactac
     3241 tcccgtcatt agttcgctga aaaaaaatta ccctgacgca aaaatcgatg tgctgcttta
     3301 tcaggacacc atcccgatcc tgtctgaaaa tccagagatt aacgcgctct acggcataaa
     3361 aaataaaaaa gcaaaagcct cagaaaaaat tgccaacttt tttcatctca tcaaggtatt
     3421 acgtgccaat aagtatgacc ttatcgtcaa tcttaccgat caatggatgg ttgctatact
     3481 ggttcgctta ttaaatgccc gtgtgaaaat ttcccaggat tatcatcatc ggcagtctgc
     3541 tttttggcgt aaaagtttca cccatttggt gccgttgcag ggtggaaatg tggtggaaag
     3601 taacttatcc gtgctgacac cattgggact tgattcgttg gtgaagcaga caaccatgag
     3661 ttacccgcct gcaagctgga aacgtatgcg tcgcgaactt gatcacgctg gtgttggaca
     3721 aaattatgtg gttatccaac ctacggcgcg gcaaatcttc aaatgctggg acaacgccaa
     3781 gttttccgct gtgattgatg ccttacatgc tcgtggttat gaagtcgttc tgacgtccgg
     3841 cccggataaa gacgatctgg cctgcgtcaa tgaaattgcg cagggatgcc agacgccacc
     3901 agtaacggcg ctggctggaa aggtgacctt cccggaactt ggtgcgttaa tcgatcatgc
     3961 gcagctgttt attggcgttg attccgcacc ggcgcatatt gccgctgcag ttaatacgcc
     4021 gctgatatcg ctgtttggtg cgacagacca tattttctgg cgtccctggt caaataacat
     4081 gattcaattc tgggcgggag attaccggga aatgccaacg cgcgatcagc gtgaccgaaa
     4141 tgagatgtat ctttcggtta ttccggcggc agatgtcatt gctgctgtcg ataaattact
     4201 gccctcctcc acgacaggta cgtcgttatg atcgttgctt tttgtttata taaatatttt
     4261 ccctttggcg gtttgcagcg tgattttatg cgtattgctc agacagtcgc cgcccgaggt
     4321 catcatgttc gggtttatac ccagtcgtgg gaaggcgaat gccctgatgt atttgaactg
     4381 atcaaagtgc cggttaaatc gcataccaat cacgggcgca atgcggagta ttttgcctgg
     4441 gtgcaaaaac atttacgcga acatcccgtc gataaagtcg ttggattcaa caaaatgccg
     4501 gggctggacg tttattatgc cgctgatgtt tgttatgccg agaaagtagc gcaggaaaaa
     4561 ggctttttct atcgcctgac gtcacgttat cgccattatg ccgcctttga gcgggcaacc
     4621 ttcgaacagg gtaagccgac acagttgctg atgctgacag ataagcaaat cgccgatttc
     4681 cagaaacatt atcagactga agcggagcgt tttcatattc tgccaccggg gatttatcct
     4741 gatcgtaaat atagccagca gccacccaat agccgtgaaa tcttccgtaa gaagaatgga
     4801 ataaccgaac aacaatattt attgttgcag gtcggttcag acttcacgcg taaaggtgtc
     4861 gatcgttcca ttgaagcact tgcttcgtta ccggattcgc tgcgccacaa cacattgcta
     4921 tatgttgttg ggcaggataa accgcgaaaa tttgaggcgc tggcagaaaa acgcggcgtg
     4981 cgcagtaatg ttcacttttt ctcggggcgc aatgatgtct cggaattaat ggcggcggcg
     5041 gatttattac tgcatcctgc ctaccaggaa gcggcgggaa ttgtgctgct ggaagcgatt
     5101 actgcaggat taccggtact aacaacagcc gtttgtggct atgcgcatta tattgtcgac
     5161 gccaattgcg gcgaggctat tgctgagcca ttccgccagg aaacattgaa tgagatttta
     5221 cgcaaagcgt taacgcaatc ttcattgcgc caggcctggg cggaaaatgc gcgacattat
     5281 gctgatacac aagatttata cagtctgcca gagaaagcgg cggatatcat aacgggtggt
     5341 ctggatggtt gaacttaaag agccgtttgc cacgttatgg cgcggcaaag atccttttga
     5401 ggaagttaaa accttgcagg gtgaggtatt tcgtgaactg gaaactcgcc gtactctgcg
     5461 ctttgaaatg gcgggcaaaa gctattttct caaatggcat cgcggcacga ccctgaaaga
     5521 gataatcaaa aatttactct cattgcggat gccagtatta ggcgcagacc gcgaatggaa
     5581 tgcgattcat cgactgcggg atgtcggcgt tgatactatg tatggggtgg cattcggcga
     5641 aaaaggcatg aatccgctga ccagaacctc gtttattatt accgaagatc tgacaccaac
     5701 cataagtctg gaagattact gtgctgactg ggcgactaac cctccagatg ttcgcgtaaa
     5761 gcgtatgctt attaagcgtg tcgcgacgat ggtgcgcgat atgcatgctg cgggcattaa
     5821 ccaccgtgac tgttatatct gtcatttcct gctgcacttg cctttttccg gtaaggaaga
     5881 ggagttaaaa atttcggtaa ttgacctgca ccgggcgcag cttcgcacgc gcgttccacg
     5941 tcgttggcgg gataaagatc ttattgggct ttatttttct tcgatgaata tcggcctgac
     6001 tcagcgggat atctggcggt ttatgaaagt gtattttgcc gccccgctta aagacattct
     6061 caagcaggaa caaggactgc tgtcgcaagc agaagcaaaa gccacaaaaa tcagggaaag
     6121 aacgattcga aaatcgttgt aatttccgga gagaaatatg agtgcccact attttaatcc
     6181 acaagagatg atcactaaga caatcatctt cgatgaaagg ccagcggcgt cagtggcatc
     6241 atcattccat gttgcttatg gcattgataa aaactttctt tttggttgtg gtgtttcaat
     6301 cacgtcagtt ttgttacata acaacgacgt gagttttgtt ttccacgttt ttattgatga
     6361 tatccctgaa gccgatatcc agcgtttagc ccaattggcg aaaagctatc gtacctgtat
     6421 ccagatccat ctggtaaatt gtgaacggct taaggcatta ccgacgacca aaaattggtc
     6481 tattgccatg tatttccgtt ttgtaattgc agattacttt attgatcaac aagataaggt
     6541 tctttacctg gatgctgata tcgcctgtca gggaaactta aagccgctga taacaatgga
     6601 tcttgccaat aacattgctg ctgttgttac tgaacgcgat gctaactggt ggtcgttacg
     6661 gggtcaaagt ctgcagtgta atgaacttga aaagggttac tttaattcag gtgtcctgtt
     6721 aattaataca ctagcgtggg cgcaggagtc cgtttctgct aaagcgatgt cgatgcttgc
     6781 tgataaagcc gtcgtttccc gtttaaccta tatggatcaa gatatactta atcttatcct
     6841 gtcagggaaa gttaaattca ttgatgctaa atacaatacg caatttagtt taaattatga
     6901 attaaaaaaa tcatttgttt gtccaattaa tgatgaaacc gtattaattc attatgtcgg
     6961 cccgacaaaa ccctggcatt actgggccgg ttatccaagt gcgcgacctt ttatcaaagc
     7021 caaagaggca tcgccctgga aaaatgaacc gttaatgcgg ccagttaact caaactatgc
     7081 tcgttattgc gccaagcata attttaaaca aaataaacca attaacggga taatgaatta
     7141 tatttattat ttttatttaa agataataaa atgaaattga gaaaagttaa atgaatgaat
     7201 ttataaaaga acggttttcg tatttagcag ataataaaaa agaaaacgcc ccagagctaa
     7261 atgtttccta cggtatcgat aagaattttt tgtatggtgc tggcgtttca atttcttccg
     7321 ttttgattaa taattcagat attaattttg tctttcatgt tttcactgat tatgtggatg
     7381 atgattattt aaagtcattt aatgaaacag caaaacaatt taatacctca attattgtat
     7441 atttaattga ccccaaatac tttgctgatc tgccgacgtc acagttttgg tcgtacgcga
     7501 catacttcag ggtattgtct tttgaatatc tgagtgaaag tatttccaca ctgctgtatc
     7561 tggatgccga tgttgtttgt aaaggaagcc tgaaacctct cacagaaatt atatttaaag
     7621 atgagtttgc tgcggtcatt cctgacaatg atagtacgca ggaggcatgt gcaaaacgcc
     7681 tcaacattcc cgaaatgaat ggacgttatt tcaatgcagg cgttatctat gtcaatctta
     7741 aaaaatggca tgaagcaaat ttgacaccgt atttactcaa gcttttacga ggggaaacta
     7801 aatatggctc tcttaaatat ttagatcagg atgcgttgaa tatcgcattt aatatgaata
     7861 atatctacct cgggaaggat tttgatacta tttataccct gaaaaatgaa cttcatgatc
     7921 gtagtcatcg aaagtttcag caaaccatta ccgataaaac agtattgatt cactatacag
     7981 ggataactaa accatggcat agctgggctg gatatccgtc tgcatcatac tttaatatcg
     8041 cgcgtgaaca atctccctgg aagaaatatc ctcttaaaga ggcgcggact gttgcagaaa
     8101 tgcagaaaca atataagcat ctgtttgccc atggtgagta tataaaaggc ataacttcat
     8161 taattaagta caagcttaag aaataaatta ctcttatgat tacaagtata cgttatcgcg
     8221 gcttctcatt ttattacaaa gataatgata ataaatataa agaaatcttt gatgaaatac
     8281 tggcctataa ttttaaaaca gtaaaagtat tacgtaatat tgatgatacc aaagtatcgt
     8341 tgattgatac gaaatatggt cgctatgttt ttaaagtctt tgctccgaaa acgaaaagga
     8401 atgaacgttt tttaaaatct tttgtaaaag gtgactacta tcagaatttg atcgttgaaa
     8461 ccgatcgtgt cagaagtgca ggtcttactt ttcctaatga tttttatttt ctggctgagc
     8521 gtaaaatttt caactatgcc agtgttttta tcatgcttat cgagtatgtc gagggcgtag
     8581 agctcaatga tatgccgatt attccagaaa atgttaaagc agaaattaag gcttcaatgg
     8641 aaaaattgca tgccttaaat atgctatcgg gcgacccaca ccggggtaac tttatcgtta
     8701 gtaaagatgg tgtcaggata attgacctat ccggtaaaag ctgtacggca gagcgtaaag
     8761 ctcgtgatcg cctggccatg gaacgtcacc tgggtattgc caacgaaata aaagattatg
     8821 gctactattc agtaatctac agaaccaaac tacgcaaatt tattaaaaaa ttaaaaggca
     8881 aagcgtaaac cacacagtca aaacggaacc aacatggatt tattagctga gagtattact
     8941 gaagtcgctg tctctgggga aattgctaac accgatcgtg tgttaaatat cgcttacggt
     9001 attgaccgta actttttatt tggtgcggca gtatctatgc aatcagttgt tatgcataac
     9061 ccggaccttg cggttaagtt tcatctcttt accgactaca ttgatgaaga ttatctacaa
     9121 cgtgttaatg cttttaccag caaaaatgct aacgttgaag taataattta taaagtctcc
     9181 aatgccttta ttgatatctt ccccagcctg aaacagtggt cttatgcaac attcttccgt
     9241 ttagttgcgt tccagtattt gagtgaaact attgaaaatc tgttatatat cgatgctgat
     9301 gtcatctgta aaggctcatt agctggattg cttgatatta attttgatga ggataagttc
     9361 gcagctgtta ttaaagatgt gccttttatg caggaaaaac cagcgaagcg tctggctata
     9421 gagggacttc cagggaatta tttcaacgcc ggtgtagtat atctgcagct tgaagcatgg
     9481 gcgaaaaatg attttatgaa taaagccatt gctatgctgg caagtgaccc gcagcacacg
     9541 aagtataaat gccttgatca ggatatttta aatattctgt tctttggtca ttgtattttt
     9601 attagcggcg attatgattg cttttatggc attgactatg agttaaaaaa taaaagcgat
     9661 gaagattata aaaagaccat taccgatgat actaagctga ttcattatgt tggcgtaacg
     9721 aagccctgga acgactggac gaattatccc tgccagaagt attttaatga ggcttatcag
     9781 gcttcttgct ggaatgatgt ggcgtttatt ccagccacga atgaaaagca gtatcaagtg
     9841 aaatatcaac atgcaaagaa aaatggtgat acgtttaacg cttttattta cttcattaaa
     9901 tttaaattaa ataagtataa aagaaaacta tttggataat aataaaaatc atggatgtct
     9961 gagttacaat aataattaag gatgagtact atcatatttt attagttcag gcaagagaac
    10021 acgatgagca atgattaccc tttagtatcc ataataatac cgacgtataa ttcatctgat
    10081 tacattactg aaactctaac gaaattagaa aaacaaactt acccaaattt tgaaattgtt
    10141 attgttaatg atggttctaa agataacaca tcaaacgttt tgagagagta tgggttaacc
    10201 cactctcgat taattattat caataaagaa aatggcggtg tttcgtctgc caggaataca
    10261 ggtatccgca aggcgcaagg acagtttata tgttttatgg atgatgatga tgagatagat
    10321 cctaactatc tgctgaagat gcattccaga caacatgaga cgggaggaga tgccatttat
    10381 tgtgggcttt atggccatca tataaaaaat ggtgttactt actcacctat aaatacagag
    10441 tttaatgaag gatctttact tttcgacttt ttttataaaa aggttagatt ccatataggg
    10501 tgcttgttta taagaaaaca acttctggaa gagaataatc ttttttttga tgaagattta
    10561 cgactaggag aagatctgga ttttatctat cgactgctaa ttacatgcga tatgtatgcg
    10621 gttccatatt atatgtataa gcataactat agagaaaatt ccttaatgaa ctcatgtaga
    10681 accatcagtc attatcgaca tgagtcattt gcgcacgaaa aaatctactc ttctgtgatg
    10741 cagttataca aaggtaaccg gaaagaagaa attcatacat tattgagtca aaatagagct
    10801 tatcataaaa ctcgttattt gtggaatgtt ctacttaatg gtgattttaa acaattgaat
    10861 caattagttg aaagcaatga aaaagaatta aaagattgta atcttcctgg caagagagat
    10921 aagagacgag caaaaatatt agcatcaaaa aattatatta tctggaggat ggtaagactg
    10981 gtaaatagaa aaaagaataa acgttagatc ttgcattaaa taacccatcc tcgtagcata
    11041 ggttgaaact atatgtcgtt ttgttggaat gaaattaatt ctggtataaa gtctttaatt
    11101 ctcatattat gtattttttc tttaatgact ttgtctttat gggatgatgt tgcaacaaag
    11161 tttcttcatg cagctggaat tatatctgca ttgtattttc ttgtgacacc aaaaaaaaca
    11221 ataactaata atcctacttt gttaattttc atctcattat gtcttttggg tatcgtaaat
    11281 atcatctggt attcacatta taaaatttca ggctctgttt ataccaatgc atatcgtggc
    11341 ccaatggaaa ctggaaaaat tgccttatgt agcgctttta ttttcttagt tctttttgct
    11401 aaaaatgaga tgagaacaaa aataaaattt gggaaactaa ttctgttcgc atccctggca
    11461 acgcagttac ttttttttgc gcatgccatg tggcaacatt tctatttaaa cgtcgaccgt
    11521 gttgcattat cagcttccca cgctacaaca gcaggctaca tcatcctttt tccttcttta
    11581 ctggcatcaa ttctcatttt aaaatccgac ttaagacata aaacaacatt atatacaatt
    11641 aacttcatgc ttagtttatg tgctgtcata gtaactgaga cgcgtgcagc catattagtg
    11701 tttccattct ttgcgttaat attaatcgta atggatagtt atattaataa gcgaattaat
    11761 tataagttat attgttttat tacgattgca ttattagcag gtgtattttc ttttaaagat
    11821 acattgctta tgagaatgaa tgacttaaat aacgatttag ttaattattc gcatgataac
    11881 accagaactt cagtcggtgc ccgtctggca atgtatgaag ttggcttaaa aacatattct
    11941 ccaataggac aatcactgga aaaacgtgcg gaaaaaatac atgaactaga agaaaaagag
    12001 cctagattga gtggcgcttt accctatgta gattctcatt tgcataacga tctcatagat
    12061 acgttatcaa cgcgtggtat tcctggagtt gtattaacaa ttttagcatt ttcagcaata
    12121 ctcatatatg ccttaagaac tgctaaagaa ccttatattt taatcttgct tttttcacta
    12181 ctggtagtag gactaagtga tgtaatactc ttttctaaac cggttccgac tgctgtgttt
    12241 atcaccataa tattgctttg tgcttatttt aaagcacaat cggaccaatg tttattagag
    12301 aagtaatgaa tatgtgaaat aaaatcagca aggaagctga ttttatttca aaattatcta
    12361 ttaaagagtt gtaatttaaa tcatggcagc gttttcttca atgaaccgct taacagcatt
    12421 tgctgtcaat tgagacaact cattccccgg agccctacaa accatctgat tcttcccata
    12481 cccaccaatt aatcccggat cggtaggtcc ataaaccgtg atattgggtc tatccagtgc
    12541 cgctgttaaa tggcttaacc ccgtatccac tgacactaca aatttagccc cggccagcac
    12601 gcgggcaacg ccttccagac tcatcttcgg caatacttca acataagcaa atccttctgc
    12661 cagtcgtttc gcccgttctt cctcatgcgg cgcgccccac ggaagtttaa tccgtattcc
    12721 tgaatcagcc agtaaaccaa tcaattctcg ccagtgttct tccggccagt gtttatcatc
    12781 acgggtcgtc gcatgaagaa atacggcata ttcgccagca tctgtaggca ggttcgtcag
    12841 gaaatgctgt gcaatagcat aatcgccctg ggtttgcggt ttgctatagc ccagactttt
    12901 ggcaaacagt tcgcgggtgc gttctacggc gtgctgctgt tttgcaatat gatgcttacg
    12961 attgtaaaac aggctggcta acggttcgcg agcggtttgc cagtccaatc catgctttac
    13021 gccatgcgcc agacgcgtca ccagcgccgc gctttttacc agcccctgag catcgataac
    13081 tgcgtcatag ttctctgctt gtagcgcttc acgaaacgct ttgcgttcag cttttatggg
    13141 ggccgagaac caggctttac gccagcgacg tattgccaca ggaataactc gctcaacggc
    13201 agcgtgccag gaaggaatct gtgcgaaccc ttcttccacc acccagtcaa acttaatccc
    13261 tgggattgcc tgctgggcat cagtgagtgc gggcaacgta tggagaacat cgcccatcga
    13321 cgatgtttta acgatcaaaa cccgcatccg tcaggcttcc tcttgtaaca atagcgcgtt
    13381 gagttcttcc agtacgcgct ggggagtaat gtcgatcaag ctctggtgat aaccctccgc
    13441 agcgtcacct ttacgcactt tgtgatagcc ggtaatcaga cggatcacgc gcgctttatg
    13501 ggatagcggc ggtgtgaagt ccgggctact cggaccaaac agggcaacca gcggacgatt
    13561 gagcgccgcc gcaacgtgca ttaggccaga atcgttagtg acaatggctt tacaggctgc
    13621 aatcaggata accgcttgat caagctgtgt ttcccccgcc aggttccgac accatgcctg
    13681 ctgctcggta ttcaaagcgg caagaatctc attgcccgct tcatgatctt tcgcagagcc
    13741 aaacagaacc acctgataac cttcatcaat cagctgcttt gccagctccg catagtggta
    13801 gtgtggccag cgttttgccg gaccaaactc cgcacccggg caaaagccaa tcatcggacg
    13861 ttctgatgaa agcgaaaatt gattacaggt atatgatttt tcaccttcgc tcacctgcaa
    13921 ctgcggccat aacaatggct gcggcagatc ttgtgcggta cgcataatgc ctttgtcata
    13981 ggccagcgcg acatagcgtt ccaccattag cggccaggct tctttatcga gcacgcgtac
    14041 atcgttgagt aaaccgtagc gcatctcgcc gcgccagcca gtgcgatgag gaatacccgc
    14101 gaagaaaggc actaatgcag atttgaagga gtttggtaag acgtaggcgc ggtcgtagcg
    14161 cttttcacgc aggctatgac ccagtttgcg gcgttcgccg atttccagcg ctccgtgacc
    14221 gagaggcata gggatagctt cgttaacttc cggcatccgc gataataatg gacggcacca
    14281 tgccggtgcc atcacatcga ttatcgcctg gggatagcgc gcctggagcg tgcgatagag
    14341 actttgcgac atcatcatgt cgccaaccca agacgggccg atcaccagta ttttcatgca
    14401 gagctcttat gcgtcgcgat tcagccaggc catgtattcc attacacctt cagcaacggt
    14461 tttgaacggt ttgtcgtaac ccgccgcgcg cagatttgtc agatctgcct gagtgaacgc
    14521 ctggtagcgg cctttcagct tatccgggaa cggaatgtat tcgatctggc ctttcttgtg
    14581 ataagccaga gtagcatctg ctacggcctg gaaggattcc gcacgaccag taccgaggtt
    14641 gaagataccg gaaacgccat tttccaggaa ccacaggttt acatctgcca cgtcgcccac
    14701 atagacgaag tcacgtttga agttctcgct accttcaaac agcttcggtg attcaccttt
    14761 gttaagctga gtgttgagat ggaaagcgac gctcgccatg ctgcctttat ggccttcacg
    14821 cggtccataa acgttgaaat agcggaagcc aacaatctgc gagttcgctt caggcaggat
    14881 ttggcgaaca tattcatcaa acaggaattt tgagtaaccg tagacgttca acggtttttc
    14941 gtactcgcgg gattcaataa agtcggaggt gcgtccgccg taggtggctg cggaagaagc
    15001 gtacaggaac gggatttcgc gctccaggca gtagtgcagc agctccttgg agtattgata
    15061 gttgttatcc atcatatact tgccgtccca ctcggtggtg gaagagcatg cgccttcgtg
    15121 gaaaatcgct tcgacatcgc cgaactcttc gccagccata atctggatca ggaagtcttc
    15181 cttatccata tagtccgcga tatccagatc caccaggttc acaaacttgg tgccgtcttt
    15241 caggttgtcc accaccagaa tatcggtgat gcctttatca ttcagggcct taacgatgtt
    15301 gctgccgata aagcccgcgc cgccggtaac gatgatcata actgtaacct tcgaattatg
    15361 gagtcagaga caatctcaga cacgaatact tctatcatat cactacacgg cgttggcttc
    15421 agccattcgc caaaagtgag attgatggac gttatttatc ctgaattttg cagaagtgtt
    15481 aacgcgttat ctcgtcgcga cctataagtt tgggtaatat gtgctggaat ttgccctgtc
    15541 tggagaatcg caatgcgtgg agaattttat cagcagttaa ccaacgatct ggaaaccgca
    15601 cgggcggaag ggttgtttaa agaagagcgc attattacgt ctgcgcagca agcagatatc
    15661 accgtggctg atggaagcca cgtcattaac ttttgtgcca acaactatct cgggctggcg
    15721 aatcatccgg atctgattgc ggcagcaaag gcgggaatgg attctcacgg tttcggcatg
    15781 gcttcggtgc gttttatttg cggcactcag gacagccata aagagcttga acaaaaactg
    15841 gcggcattcc tggggatgga agatgcgatt ctctactctt cctgctttga tgctaacggt
    15901 ggcctgtttg aaacgctgct gggcgcggaa gacgccatta tctccgacgc actgaaccac
    15961 gcgtctatta ttgatggtgt gcgtctgtgt aaagctaaac gctatcgcta tgccaacaac
    16021 gatatgcagg agcttgaagc tcgtctgaaa gaagcgcgtg aagccggtgc gcgtcatgtg
    16081 ctgattgcca ccgatggtgt gttctcgatg gacggcgtga ttgccaatct gaagggcgtt
    16141 tgcgatctgg cagataaata cgatgccctg gtgatggtag acgactccca cgcggtcggt
    16201 tttgtcggtg aaaatggtcg tggttcccat gaatactgcg atgtgatggg gcgtgtcgac
    16261 atcatcaccg gcacgctcgg taaagcgctg ggcggggctt ctggtggtta taccgcggcg
    16321 cgcaaagaag tggttgagtg gctgcgccag cgttctcgtc cgtacctgtt ctccaactcg
    16381 ctggcaccag cgattgtcgc cgcttccatc aaagtgctgg agatggttga agcgggtagc
    16441 gagttgcgtg accgtctgtg ggcgaacgcg cgtcagttcc gtgagcaaat gtcggcggcg
    16501 ggctttaccc tggcgggagc cgatcacgcc attattccgg tcatgcttgg tgatgcggta
    16561 gtggcgcaga aatttgcccg tgagctgcaa aaagagggca tttacgtcac cggtttcttc
    16621 tatccggtcg ttccgaaagg tcaggcgcgt attcgtaccc agatgtctgc ggcgcatacc
    16681 cctgagcaaa ttacgcgtgc agtagaagca ttcacgcgta ttggtaaaca actgggcgtt
    16741 atcgcctgag gatgtgagat gaaagcgtta tccaaactga aagcggaaga gggcatctgg
    16801 atgaccgacg ttcctgtacc ggaactcggg cataacgatc tgctgattaa aatccgtaaa
    16861 acagccatct gcgggactga cgttcacatc tataactggg atgagtggtc gcaaaaaacc
    16921 atcccggtgc cgatggtcgt gggccatgaa tatgtcggtg aagtggtagg tattggtcag
    16981 gaagtgaagg gcttcaagat tggcgatcgc gtttctggcg aaggccatat cacctgcggt
    17041 cactgtcgca actgtcgtgg cggtcgtacc catctgtgcc gcaacacgat cggcgtcggc
    17101 gttaaccgcc cgggctgctt cgccgaatat ctggtgattc cggcgttcaa cgccttcaaa
    17161 attcccgaca atatctccga cgacctggct tccatttttg atcccttcgg taatgccgtg
    17221 cataccgcgc tgtcgttcga tctggttggc gaggatgtgc tggtttctgg tgcaggcccg
    17281 attggtatta tggcagcggc ggtggcgaaa cacgttggtg cacgcaatgt ggtgatcact
    17341 gatgttaacg aataccgcct tgagctggcg cgtaaaatgg gtatcacccg tgcggttaac
    17401 gtcgccaaag aaaatctcaa tgacgtgatg gcggagttag gcatgaccga aggttttgat
    17461 gtcggtctgg aaatgtccgg tgcgccacca gcgtttcgta ccatgcttga taccatgaac
    17521 cacggcggcc gtattgcgat gctgggtatt ccgccgtctg atatgtctat cgactggacc
    17581 aaagtgatct ttaaaggctt gttcattaaa ggtatttacg gtcgtgagat gtttgaaacc
    17641 tggtacaaga tggcggcgct gattcagtct ggcctcgatc tttcgccgat cattacccat
    17701 cgtttctcta tcgatgattt ccagaagggc tttgacgcta tgcgttcggg ccagtccggg
    17761 aaagtaattc tgagttggg
//
LOCUS       DAMZET010000087        17381 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.087, whole genome shotgun sequence.
ACCESSION   DAMZET010000087 DAMZET010000000
VERSION     DAMZET010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.087"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            65..1183
                     /gene="hybO"
                     /locus_tag="POB34_002921"
     CDS             65..1183
                     /gene="hybO"
                     /locus_tag="POB34_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HDI5919828.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            1186..2172
                     /gene="hybA"
                     /locus_tag="POB34_002922"
     CDS             1186..2172
                     /gene="hybA"
                     /locus_tag="POB34_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HDI5919829.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            2162..3340
                     /gene="hybB"
                     /locus_tag="POB34_002923"
     CDS             2162..3340
                     /gene="hybB"
                     /locus_tag="POB34_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HDI5919830.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            3337..5040
                     /gene="hybC"
                     /locus_tag="POB34_002924"
     CDS             3337..5040
                     /gene="hybC"
                     /locus_tag="POB34_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HDI5919831.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            5040..5534
                     /locus_tag="POB34_002925"
     CDS             5040..5534
                     /locus_tag="POB34_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HDI5919832.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            5527..6015
                     /gene="hybE"
                     /locus_tag="POB34_002926"
     CDS             5527..6015
                     /gene="hybE"
                     /locus_tag="POB34_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HDI5919833.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            6008..6349
                     /gene="hypA"
                     /locus_tag="POB34_002927"
     CDS             6008..6349
                     /gene="hypA"
                     /locus_tag="POB34_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDI5919834.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            6362..6610
                     /gene="hybG"
                     /locus_tag="POB34_002928"
     CDS             6362..6610
                     /gene="hybG"
                     /locus_tag="POB34_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HDI5919835.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(6733..7599)
                     /gene="yghU"
                     /locus_tag="POB34_002929"
     CDS             complement(6733..7599)
                     /gene="yghU"
                     /locus_tag="POB34_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HDI5919836.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            7804..9663
                     /gene="gss"
                     /locus_tag="POB34_002930"
     CDS             7804..9663
                     /gene="gss"
                     /locus_tag="POB34_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HDI5919837.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDLLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            9955..11454
                     /gene="pitB"
                     /locus_tag="POB34_002931"
     CDS             9955..11454
                     /gene="pitB"
                     /locus_tag="POB34_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HDI5919838.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(11503..12195)
                     /locus_tag="POB34_002932"
     CDS             complement(11503..12195)
                     /locus_tag="POB34_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDI5919839.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            12369..13082
                     /locus_tag="POB34_002933"
     CDS             12369..13082
                     /locus_tag="POB34_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDI5919840.1"
                     /translation="MNQRNMSIINSTPVHVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEILQASLRAIHAALS"
     gene            13114..13872
                     /locus_tag="POB34_002934"
     CDS             13114..13872
                     /locus_tag="POB34_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919841.1"
                     /translation="MDALQTQTVISTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            13918..15267
                     /locus_tag="POB34_002935"
     CDS             13918..15267
                     /locus_tag="POB34_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HDI5919842.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPHQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVFI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            15267..16091
                     /locus_tag="POB34_002936"
     CDS             15267..16091
                     /locus_tag="POB34_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919843.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            16103..16663
                     /locus_tag="POB34_002937"
     CDS             16103..16663
                     /locus_tag="POB34_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDI5919844.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            16694..>17381
                     /locus_tag="POB34_002938"
     CDS             16694..>17381
                     /locus_tag="POB34_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HDI5919845.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNL"
BASE COUNT         4056 a         4276 c         4695 g         4354 t
ORIGIN      
        1 cgcattattg attttgcgat tgaggcaaaa tatatgccag gtcttcgcaa cggaataact
       61 ataaatgact ggagataaca ccctcatcca ttctcacggc attaaccgtc gtgatttcat
      121 gaagctttgt gcagcattag ccgccaccat ggggttaagt agcaaagccg ctgcagagat
      181 ggccgaatcg gttactaacc cgcagcgtcc gccagttatc tggattggcg cgcaggagtg
      241 caccggttgt acggaatctc tgcttcgtgc aacgcatcca acggtagaaa acctcgtgct
      301 ggagactatc tctctggagt atcacgaagt gctttccgcc gccttcggtc atcaggtcga
      361 agagaacaaa cataacgcac ttgagaagta caaagggcag tatgtgttgg ttgtggatgg
      421 ttccatccca ttaaaagata acggtattta ttgcatggtt gctggtgagc cgattgtgga
      481 tcacatccgc aaagcggcag aaggcgcagc agcgattatc gctatcggtt cctgctctgc
      541 gtggggcggt gttgccgcag ctggagttaa cccaactggc gcagtcagcc tgcaagaagt
      601 tctgccaggc aaaaccgtta tcaatattcc gggctgcccg ccgaacccgc acaacttcct
      661 cgcgaccgtt gcgcacatca tcacttacgg caaaccgccg aaactggatg acaaaaatcg
      721 tccgaccttc gcctatggcc gtctgattca cgaacactgc gaacgtcgcc cgcacttcga
      781 tgctggtcgt tttgccaaag agttcggtga tgaaggccac cgtgaaggct ggtgcctgta
      841 ccacctcggc tgtaaagggc cagaaactta cggcaactgc tcaacgctgc aattctgcga
      901 tgttggcggc gtgtggccgg tagcgattgg tcacccatgc tatggctgta acgaagaagg
      961 tatcggcttc cataaaggca tccatcagct tgccaacgtc gaaaatcaga ctccgcgttc
     1021 acagaaaccg gatgttaacg ctaaagaggg cggcaacgtc tctgcaggcg ctattggttt
     1081 gctcggcggt gtggttgggc tcgttgccgg tgtcagcgtg atggcggtgc gtgaactggg
     1141 tcgtcagcaa aagaaagata acgctgactc acggggagaa taaccgtgaa cagacgtaat
     1201 tttattaaag cagcctcctg cggggcattg ctgacgggcg cgctgccgtc tgtcagtcat
     1261 gcggctgctg aaaaccgccc gccaattccg ggatcgctgg ggatgttgta cgactcgacc
     1321 ttgtgcgtag gctgccaggc ttgcgtcacc aagtgtcagg atatcaactt ccctgaacgt
     1381 aacccgcaag gggaacagac ctggtcgaac aacgacaaac tgtcgccgta taccaataac
     1441 atcattcagg tgtggaccag cggcacaggg gtcaacaaag accaggagga gaacggctac
     1501 gcgtacatta agaaacagtg tatgcactgc gtcgatccga actgtgtctc tgtgtgcccg
     1561 gtctctgcac tgaaaaaaga tccgaaaacc ggcattgtcc attacgacaa agacgtgtgc
     1621 accggctgcc gttactgcat ggtcgcctgt ccgtacaacg tgccgaagta cgactacaac
     1681 aacccgtttg gtgcgctgca taagtgcgag ctgtgcaacc agaaaggtgt ggaacgtctc
     1741 gataaaggcg gtctgcctgg ctgcgtagaa gtgtgcccgg cgggcgcggt gattttcggt
     1801 acgcgtgaag agctgatggc ggaggcgaaa aaacgtctgg cgctgaagcc tggcagcgaa
     1861 taccactatc cgcgtcagac gctgaaatct ggcgacactt acctgcatac ggtgccgaaa
     1921 tattatccgc atctgtacgg cgagaaagag ggcggcggta ctcaggttct ggtactgacg
     1981 ggtgtgcctt atgaaaatct cgacctgccg aaactggacg atctttctac cggtgcgcgt
     2041 tccgaaaata ttcaacacac cctgtataaa ggcatgatgc taccactggc tgtgctggcg
     2101 ggcttaaccg tgctggttcg tcgcaacacc aaaaacgacc atcacgacgg aggagacgat
     2161 catgagtcat gatccacaac cgctgggcgg caaaatcatc agtaaaccgg tcatgatttt
     2221 tggaccgtta atcgtcatct gtatgctcct gattgtgaag cgtctggtgt tcggtctggg
     2281 ctctgtctct gacctgaacg gcggcttccc gtggggcgtg tggatcgcgt ttgacctgct
     2341 gattggcacc ggctttgcct gtggcggctg ggcgctggcg tgggcggtat acgtctttaa
     2401 ccgtgggcaa taccatccgc tggtgcgtcc ggcgctgttg gcgagtctgt ttggttactc
     2461 actgggtggc ttgtcgatca ctatcgacgt gggtcgctac tggaacctgc cgtacttcta
     2521 cattccgggc cacttcaacg tgaactcggt actgttcgag acggcggtct gtatgaccat
     2581 ctatatcggc gtgatggcac tggagtttgc tccggcactg tttgaacgtc tggggtggaa
     2641 ggtgtcgcta cagcgactaa acaaggtgat gttcttcatc atcgcgctcg gtgcgctgct
     2701 gccgaccatg caccagtctt caatggggtc gctgatgatc tcggcgggct acaaggtgca
     2761 tccgttgtgg cagagctatg aaatgttgcc gctgttctcg ctgctgacgg cgttcatcat
     2821 gggcttctcg attgtcatct ttgaaggttc gctggtgcag gcgggtctgc gtggcaacgg
     2881 tccggatgaa aagagtctgt ttgttaagct gaccaacacc atcagtgtgt tgctggcgat
     2941 tttcatcgtg ctgcgctttg gcgagcttat ctatcgcgac aagctgtcgt tagcgtttgc
     3001 cggtgacttc tactccgtga tgttctggat tgaagtcctg ctgatgctct tcccgctggt
     3061 cgttctgcgt gtggcgaagc tgcgtaatga ttcccgcatg ctgttcctgt cagcactgag
     3121 cgcactgtta ggttgtgcaa cctggcgtct gacctattcg ctggtggcat tcaacccggg
     3181 cggcggttac gcctacttcc cgacctggga agaactgttg atttctattg gttttgtggc
     3241 tattgagatt tgcgcttaca tcgtactcat tcgtctactg ccgatacttc ctcctttaaa
     3301 acaaaacgat cataatcgtc atgaggcgag caaagcatga gccagagaat tactattgat
     3361 ccggtaaccc gtattgaggg gcatttacgc atcgattgcg aaatcgaaaa tggcgtcgtt
     3421 tcgaaagcat gggcttccgg taccatgtgg cgcggcatgg aagagatcgt gaaaaaccgc
     3481 gatccgcgcg atgcatggat gattgtgcaa cgtatctgtg gcgtatgtac taccactcac
     3541 gcgctgtctt ccgttcgtgc ggcagaaagt gcgctgaata tcgacgttcc ggttaacgcg
     3601 caatacatcc gtaacatcat tctggctgcg cacaccacgc atgaccatat tgttcatttc
     3661 tatcagcttt cggcgctgga ctgggtggac atcacttctg cactgcaagc tgacccaacc
     3721 aaagcctccg aaatgctgaa aggcgtttcg acctggcacc tgaacagtcc ggaagagttc
     3781 accaaagttc agaacaagat caaagatctg gttgccagcg gtcagttggg tattttcgcc
     3841 aatggctact ggggtcaccc ggcgatgaaa ctgccgccgg aagtgaacct gattgcggta
     3901 gcgcactacc tgcaggcgct ggagtgccag cgtgacgcta accgcgtcgt ggcgctgctg
     3961 ggcggtaaaa cgccgcacat tcagaacctg gcggtaggtg gtgtcgcgaa cccgatcaac
     4021 ctcgacggtt tgggcgtgct gaaccttgag cgcctgatgt acatcaagtc tttcatcgac
     4081 aagctgagcg actttgttga acaggtttat aaggttgata ccgcagttat tgccgcgttc
     4141 tacccggaat ggctgacgcg cggtaaaggt gcggtgaact acctgagcgt gccggaattc
     4201 ccgaccgaca gtaaaaacgg cagcttcctg ttcccgggcg gctacattga gaatgcggat
     4261 ctgtcctcgt atcgtccgat cacttctcat tccgatgaat acctgatcaa aggcattcag
     4321 gaaagcgcga agcactcctg gtataaagac gaagcgccgc aggcaccgtg ggaaggtacc
     4381 accattccgg cttatgatgg ttggtctgac gacgggaaat attcctgggt gaaatcaccg
     4441 actttctacg gcaaaacggt agaagtgggt ccgctggcta acatgctggt gaaactggcg
     4501 gcaggacgcg agtctaccca gaacaaactg aatgaaatcg ttgcgattta tcagaaactg
     4561 actggtaaca cgcttgaagt ggcgcaactg cactctacgc tgggccgtat tattggtcgt
     4621 accgttcact gctgcgaatt gcaggatatc ctgcaaaatc aatacagtgc actgatcacc
     4681 aatatcggta aaggcgatca taccaccttt gtgaagccga acattccggc aacgggtgag
     4741 ttcaaaggtg ttggcttcct cgaagcgccg cgcggtatgc tctctcactg gatggtgatc
     4801 aaagacggta tcatcagtaa ctaccaggcg gttgttccat caacctggaa ctctggtccg
     4861 cgtaacttca atgatgacgt cggtccttac gagcagtcgc tggtgggtac accggttgcc
     4921 gatccgaata aaccgctgga agtggtgcgt accattcact ccttcgaccc gtgcatggcc
     4981 tgtgcggtac acgtagtgga tgctgacggc aacgaagtgg tttcagtgaa ggttctgtaa
     5041 tgcgtatttt agtcttaggg gtcggcaata ttttgctgac cgatgaagcc atcggtgtgc
     5101 ggattgtcga agcgttagag caacgataca ttctgccgga ttatgttgag atcctcgatg
     5161 gcggcacggc gggaatggag ctgcttggcg acatggcaaa tcgcgatcat ctgattattg
     5221 cggatgccat tgtgtcgaag aagagcgcgc cgggaacgat gatgatcctg cgcgatgaag
     5281 aagttccggc gttgtttacc aacaaaatct ctccgcatca gcttggcctg gccgacgtct
     5341 tgtcggccct gcgcttcacc ggtgagtttc cgaaaaagct gaccctggtc ggcgtgatcc
     5401 cggaatcgct ggagccacac atcggcttaa cgccgacggt tgaagcaatg attgaacctg
     5461 cgcttgagca ggttctggct gcgctgcgtg aatctggcgt ggaagccatc ccacgggagg
     5521 cgattcatga ctgaagagat agcaggtttc cagacctccc cgaaggcgca agtacaggca
     5581 gcgtttgaag aaattgcccg gcgttcgatg cacgatctct cttttctgca tccttcaatg
     5641 ccggtgtatg tttctgattt tacgctgttc gaaggtcagt ggacggggtg tgtgatcacc
     5701 ccgtggatgc tgagtgcagt tatcttcccc ggcccggatc aactctggcc gctgcgcaaa
     5761 gtgagtgaaa aaattggtct gcaactgccg tatggcacta tgacctttac cgttggcgaa
     5821 ctggacggtg tttcgcaata tctctcctgt tcgctgatgt cgccgctttc gcacagcatg
     5881 tcgattgaag agggccaacg cctgacggat gactgcgcac gaatgatcct ttcgctgcca
     5941 gtcacgaatc cggatgtacc acacgcaggg cgtcgcgccc tgctgtttgg tcgcaggagt
     6001 ggcgaaaatg catgagttgt ctctttgcca gagcgccgtt gaaattatcc aacggcaggc
     6061 ggagcagcac gatgttaagc gcgtcaccgc cgtgtggctg gaaattggcg cgctctcctg
     6121 cgttgaggag agcgccgtcc gttttagttt tgaaattgtc tgccacggaa cggtggcgca
     6181 agggtgcgat ttacatatcg tctataaacc cgcccaggcc tggtgctggg attgcagcca
     6241 ggtggtggag attcatcagc acgatgcgca gtgtccgctc tgtcacggcg agcggttgcg
     6301 tgtcgatacc ggcgattcgc tgatcgtcaa aagtattgaa gttgaataac cggagttaat
     6361 aatgtgtatt ggcgttccag gccaggtgct ggctgtcggt gaagatattc accagcttgc
     6421 gcaggttgaa gtatgtggta tcaagcgcga cgtgaatatc gccctgattt gtgaaggtaa
     6481 ccctgccgat ctactgggcc agtgggtgct ggtacacgtc ggatttgcca tgagcatcat
     6541 cgacgaagat gaagccaaag ccacattaga cgcactgcgc caaatggatt acgacattac
     6601 cagcgcgtga tgattagctt tcgtaggcct gataagacgc ggcagcgtcg catcaggcat
     6661 tgtgcacgat tgccggatgc ggcgtgaacg ccttatccgg cctggatggc ttgctgcgac
     6721 gaacaccaac ccttacccct gacgcttatc ttccgtattc gtctcgaaat cactggcgtc
     6781 atggcgctca tgcaactgct cattcagcgg tccgttggtg cggttaacaa tacgcccacg
     6841 tttcaccgcc ggacgttcgc ctacttcttt cgcccagcgt tgtacatgct tataactgcc
     6901 cgcatcaaga aactcagcgg catcatacac accacctaac accacgttgc caaaccacgg
     6961 ccaaatcgcc atatccgcaa tggtgtactc atcgcccgca acaaacttat gctgcgccag
     7021 ttgcttatcc agcacgtcga gcagacgttt ggcttccatg gtaaagcggt tgatggcgta
     7081 ctcaatcttt accggtgcgt aatggtaaaa gtgaccaaaa ccaccgccga ggaacggtgc
     7141 cgcgccctgt aaccagaaca gccagttcat cgtttcagta cgctttgcca aatcctgcgg
     7201 caggaagtag ccaaatttct ccgccagata aagcaggatc gaaccagatt caaacacgcg
     7261 gatcggcgga ttatgcgtat gatcgcgcag cgccgggatc ttcgagtttg ggttcacttc
     7321 gacaaagccg ctggagaatt gatcgccatc gccaatacga atcagccagg cgtcgtactc
     7381 tgcaccagta acgcccagcg ccagcagctc ctcaagcata atcgttactt tctgaccgtt
     7441 cggcgttccc agcgaataaa gttgcaatgg gtgtttgcca acgggcagcg ttttttcatg
     7501 cgtcggacca gaaaccgggc gattgatatt ggcgaacgcg ccgccagcgg atttatccca
     7561 cgtccagact ttcgcgggct gataagtatt gtctgtcata gtgagttgcc ttctgagtgg
     7621 tagtgttgaa gcagtgtagc aggtcacttg ctgtacagaa tgtgaatttt ttaacccttt
     7681 gcgccagccg ctgcgatgag gtgtatgatt agtctgacct ctgtccggtt aatgtcgctg
     7741 agcaggtaat cagagcacaa caattacgcc aactgcttag gttgtattgt taaaggtaaa
     7801 gtgatgagca aaggaacgac cagccaggat gccccgttcg ggacattatt gggctacgcc
     7861 ccaggtgggg tagcaatcta ctcttcagat tacagttctc tcgatccgca ggaatacgaa
     7921 gatgacgccg tattccgtag ctatatcgac gacgaatata tgggccacaa gtggcaatgc
     7981 gttgaatttg ctcgccgttt tctctttctg aattacggtg tggtctttac tgacgtgggt
     8041 atggcgtggg agattttctc gctgcgcttc ctgcgagaag tggttaatga caacatcctg
     8101 ccattgcagg catttcctaa cggctcgccg cgtgcgccgg tcgcgggtgc gcttcttatc
     8161 tgggataaag gcggtgaatt taaagacact ggccatgtcg ccatcattac ccaattgcat
     8221 ggcaacaaag tccgtattgc ggaacagaac gtgattcatt ccccgttgcc gcaagggcaa
     8281 cagtggacgc gtgagctgga gatggtggtc gaaaacggct gctataccct gaaagacact
     8341 tttgatgaca ccaccattct gggctggatg atccagacgg aagatactga atacagctta
     8401 ccgcagccgg aaattgcagg cgagctgctg aaaatcagcg gagcgcgcct ggaaaacaaa
     8461 ggccagtttg acggtaaatg gctggatgaa aaagatctgc tgcaaaacgc ctatgtgcag
     8521 gccaacggtc aggtgatcaa tcaggatcct tatcattact acaccattac cgagagtgcc
     8581 gagcaggagc taattaaagc caccaacgag ctgcacctga tgtatcttca cgcaaccgac
     8641 aaggtgctga aagatgacaa cctgctggcg ctgttcgaca tcccgaaaat cctctggcca
     8701 cgtttgcgtc tctcctggca gcgtcgccgt caccatatga tcactggtcg tatggatttc
     8761 tgcatggatg agcgtggcct gaaggtttac gaatacaacg ccgactctgc ctcatgtcat
     8821 accgaagcgg gcttgatcct cgaacgttgg gcggagcagg gctataaagg caacggcttc
     8881 aatccggcgg aagggctgat taacgaactg gctggtgcct ggaaacacag tcgtgcacgt
     8941 ccgtttgtcc atatcatgca ggacaaagat atcgaggaaa actatcacgc gcagtttatg
     9001 gagcaggcgc tgcaccaggc gggctttgaa acgcgtatct tgcgcgggct ggatgaactg
     9061 ggctgggatg ctgccgggca actgattgat ggggaaggac ggctggttaa ctgcgtgtgg
     9121 aaaacctggg cgtgggaaac cgcgtttgat cagattcgtg aagttagcga ccgtgagttt
     9181 gctgcggtgc caatccgtac cggtcatccg caaaacgaag tgcgtcttat cgacgtattg
     9241 ctgcgcccgg aagtgctggt ctttgagccg ctgtggacgg tgatccccgg caacaaagcg
     9301 attctgccga tcctctggtc gctgttcccg caccatcgtt acctgctgga taccgatttc
     9361 actgttaatg atgaactggt gaaaacaggt tacgcagtga aaccgatcgc cggtcgctgt
     9421 ggcagcaata tcgacctcgt cagccatcat gaagaggtgc tggacaaaac cagcggtaaa
     9481 tttgccgagc agaaaaacat ctatcagcaa ctgtggtgtt tgccgaaagt ggacggtaaa
     9541 tacattcagg tatgtacctt caccgttggc ggcaactacg gtgggacgtg tttgcgcggt
     9601 gatgaatcac tggtcatcaa aaaagagagt gatattgaac cgttaattgt ggtgaaaaag
     9661 taatacatgt tgtagcaacc ccgtactgat tatgaagaat aatcagtacg gggattaatc
     9721 aaaatattca atatttatat attccaatat ttattcattt catatgtgaa tatattaacc
     9781 agtggaatac ctgtgttttg attatttcta aaggttttga atgaatgctt attgtctgat
     9841 acacaagaaa taacactctt tttatcgtta aaaaatgata tttcactttg cccatgccgt
     9901 taaagtcact gataatgcgt ccgttcgtaa attcaaaatg gcgtaatcta atatatgcta
     9961 aatttatttg ttggccttga tatatacaca gggcttttgt tattgcttgc tctggcattt
    10021 gtgttgttct acgaagcaat caatggtttt catgacacgg cgaatgcggt ggcaaccgtt
    10081 atttatactc gtgccatgca accacaactt gctgtggtga tggcggcatt ttttaacttt
    10141 tttggcgtgt tattgggcgg acttagcgtt gcctatgcca ttgtccatat gttgccaacc
    10201 gatttgttgc tgaatatggg gtcaacccac ggcctggcga tggtcttttc catgctgctg
    10261 gcggcgatta tctggaacct gggaacgtgg ttcttcggtt taccggcctc cagttcgcac
    10321 accttgattg gtgcgattat cggcatcggt ttaaccaacg cgctgttaac cggctcatcg
    10381 gtgatggatg cgttaaacct gcgtgaagtg accaaaattt tctcctcgct gattgtttcc
    10441 cctatcgtcg gcctggtcat tgcgggaggc ctgatattcc tgctgcgacg ctactggagc
    10501 gggacgaaaa agcgtgaccg tattcaccgc attccggaag atcgcaaaaa gaaaaaaggc
    10561 aaacgtaaac cgccattctg gacgcgtatt gcgctgattg tttccgctgc gggcgtggcg
    10621 ttttcgcacg gcgcgaacga cggacaaaaa gggatcggcc tggtgatgct ggtactggtg
    10681 gggattgccc ctgctggctt cgtcgtcaat atgaacgcgt ccggctatga aattacccgt
    10741 acccgcgatg ccgtcaccaa cttcgaacac tacctgcaac agcatcctga actgccgcag
    10801 aagttgattg cgatggaacc tccattgcct gcagcatcga ctgatggcac gcaagtaaca
    10861 gagtttcact gtcatccggc aaataccttt gatgcgattg cgcgcgttaa aacgatgctg
    10921 ccaggcaata tggaaagtta cgagccgtta agcgtgagtc agcgcagcca gctgcgccgc
    10981 attatgctgt gcatctctga tacctccgcg aagctggcga aactgccagg cgtcagtaaa
    11041 gaagaccaga acctgctgaa aaaactgcgc agcgatatgt taagcaccat tgagtacgct
    11101 ccggtgtgga tcatcatggc ggtagcactg gcgctcggca ttggcaccat gattggctgg
    11161 cgtcgtgtag cgatgaccat cggtgagaag attggtaagc gcggcatgac gtatgcgcaa
    11221 ggcatggcgg cacaaatgac ggcggcagtg tctatcggtc ttgccagtta tattgggatg
    11281 cccgtctcca caacacacgt cctctcgtct gcagttgcag ggacgatggt ggtggacggc
    11341 ggtgggttac agcgtaaaac ggtaaccagc atcctgatgg cgtgggtatt tactttaccg
    11401 gcggcaattt ttctttctgg tgggctgtac tggatagcat tgcagttgat ttaaccttcc
    11461 tgaaaatgcc cggtcctggc gatcgggcat ttccattttt gactagtgat aaccacgcgc
    11521 ggtcataaaa tccgtaatcg ctttttctgc atcaaccaac acctgttcca gtggctgatt
    11581 ggcatcgata tcaaccagtt gtgcaccacc aaaggttaac tgtggcgtta tggcaatctt
    11641 cctcgccagc gattcccgtt tatggtcggg tttacgtgca caggcaactt caaggtcaac
    11701 attgagtttg atgaccagat caggcttatg gctcgccatc cagtgaaacg ctttacgttc
    11761 ctggcttgcc agccatgaga caaaacgacc accttcaacg ttaggtggga acaccgtacc
    11821 atcgtaagcg ccaggaattt ggtcctgagg ataacggtcg gttagaacaa ttaacccgcg
    11881 acgacgacag gcaagcatat gacgaaagcg cagtaagcga cgggcgacaa acgctgtaat
    11941 taccagcgcc ggaactggtc caggcaattt ttttgctgtt ttcacctgat ttcgttcaat
    12001 tgttttatgt aaggattttc ccatcaacgg taattttgtc actgcacgac cgacatttcc
    12061 ggcctgtttt cctaaatgaa ctctttcggc agcaccatat ttttcgacaa cggtaataag
    12121 atgttcacac accgttgact tgcctgaacc atcgctacca ataacggcaa ttaatggagg
    12181 tgttattgac tgcatgaata tatcctttaa tttaaatatc cattaaaaat atttatttgg
    12241 ttaatatgtt tttatgaaag cgtaattcag gtcaatgtca caattaacca ttgtcacaat
    12301 aaggttgaac ggatattctg agcacggacc acgtaatatt caatacatta tttactgccg
    12361 ggtttttcgt gaatcaacgt aatatgtcaa taattaattc cacgccagtg catgttattg
    12421 ccattgtagg atgtgatggt tcaggtaaat cgaccctcac ggcaagcctg gtaaatgaac
    12481 tggcagcaag aatgccaaca gaacacattt atctcgggca atcgtccggg cgaattggcg
    12541 aatggatttc acagctccct gttattggcg caccttttgg gcgttatctg cgaagtaaag
    12601 cggcacatgt gcacgaaaag ccctcaacac cgcctggcaa tattactgca ctggttatct
    12661 atctgctttc ctgctggcgg gcgtacaagt ttcgcaaaat gttgtgtaaa agccagcaag
    12721 gctttctgct catcaccgac cgctacccgc aggttgaagt gccggggttt cgctttgatg
    12781 gcccgcaatt ggcaaaaacc acgggcggta acggttggat aaaaatgtta aggcagcgcg
    12841 agctgaagct gtaccaatgg atggcatctt atttgcccgt attgttgatt cgtcttggca
    12901 ttgatgaaca aacggcgttt gcgcgtaaac ctgaccacca actggcagcg ttacaggaaa
    12961 aaatcgccgt tacgccgcaa ctgacattca atggtgcaaa gatccttgaa ctggatggtc
    13021 ggcaaccagc cgatgaaatt ctgcaagcgt cactacgcgc aattcacgcc gcgctttcct
    13081 gatcaccaaa atggataggg gatgatttaa cgaatggatg cactacaaac acaaactgtt
    13141 attagtacaa ccgcgccgca gcccagctac attccggggc taattgcggt ggtcgggtgt
    13201 gatggcaccg gtaaatccac actgaccacc gacctggtga aatcgctgca acaacactgg
    13261 caaaccgagc ggcgctatct ggggctgctc tccggcgaag acggcgacaa aatcaaacga
    13321 ttgccgttgg ttggcgtctg gctggaacgg cgactggcgg ccaaatcctc gaaaacccaa
    13381 agcatgaaaa ccaaatctcc ggcgctatgg gcggcggtga ttatgtactg cttctcgctg
    13441 cgaagaatgg cgaatctacg caaggttcag cgactggcgc aaagtggcgt tctggtggtc
    13501 agcgatcgct tcccgcaggc tgaaatttcg ggcttttatt atgacggacc ggggattggc
    13561 gtcgaacgtg cgaccgggaa aatcagcatg tttctggcgc agcgcgaacg gcgtttatac
    13621 caacaaatgg cgcaatatcg cccggaatta attattcgcc tgggcattga tattgagact
    13681 gccatctccc gcaagcctga ccatgactat gccgagctgc aggacaaaat cggtgtcatg
    13741 tcgaagattg gctataacgg cacaaaaatt cttgagattg attccagagc gccctacagc
    13801 gaagtgctgg aacaggcaca gaaggcggtt tctctggttg ccatcgtttc tgaccgccga
    13861 agtttaactt aaggctggcg ggatactttt tactatcaac gataaggtca ggctgaattg
    13921 gcgggtttta acatcaaaca ttggtttgca gatggcgcgt ttcgcaccat tattcgcaat
    13981 agcgcctggt taggctccag taatgtcgtg agcgccttgc tggggctgtt ggcgctctcg
    14041 tgtgccggta aagggatgac gcccgccatg tttggcgtac tggtgattgt gcaatcgtac
    14101 gccaagtcga tcagcgattt tattaagttt cagacatggc agctggtggt tcagtacgga
    14161 acgccagcct tgaccaacaa taatccgcac caatttcgca atgtcgtctc attttccttc
    14221 tcgctggata tcgtcagcgg cgcggtggcg attgtcggtg gcattgcctt actgccattc
    14281 ctttcccatt cattaggtct ggatgaccaa agtttttggc tggcagcgct ctattgcacg
    14341 ctcattcctt caatggcttc ctccacgccg accggcattc tgcgtgcggt agatcgcttc
    14401 gatttaattg ctgtacagca ggcgacgaaa ccttttctgc gcgcagcggg gagcgtcgta
    14461 gcctggtatt ttgactttgg ttttgcgggt tttgttattg cctggtacgt gtcgaatctg
    14521 gttggcggca ccatgtactg gtggtttgcc gcgcgcgaat tacgccgccg aaatatccat
    14581 aatgccttca aattgaatct gtttgagtct gcccgacaca tcaaaggcgc gtggagtttt
    14641 gtctggtcaa ccaacattgc ccactccatc tggtcggcgc gtaactcgtg cagcacagtg
    14701 ctggtgggga tcgtgttagg acccgctgcc gccgggttat ttaaaatcgc catgacattc
    14761 ttcgacgccg ccggaacgcc agcgggtttg ctgggtaaaa gtttttaccc agaggtgatg
    14821 cgtttagatc cgcgcaccac cagaccgtgg ttgttaggtg tgaagtccgg tttactggcg
    14881 ggcggaatcg gtattctggt ggcgctcgcg gtgtttatcg tcggcaagcc gctcatttcg
    14941 ctggtgtttg gcgttaagta tctcgaagcg tatgacctga ttcaggtgat gctgggcgca
    15001 attgtgattt cgatgctggg cttccctcag gagtcattgc tattgatggc gggaaaacag
    15061 cgcgcttttc tcgtggcgca aaccatcgcc tcaatcggct acatcgtatt gctgttcatg
    15121 ttctgtcatc tgtttggtgt gctgggagca gcgtttgctt acttcggtgg gcaatgtctg
    15181 gacgtggcgc tctcgttaat tccaacctta aaggcatttt ttcagcgcca ttccttgctt
    15241 tataacgcag ccggagagaa atcctgatga tgagaaaata tttcccgctt gaggcgagcg
    15301 agcgtctatt tgttgcgatt gaagaagatg atgtcgttga tgcacaggtt agtttgccgc
    15361 cgactattgc actaagttgt accaccgaaa tcattcatga taattacgcg ttatgcctgc
    15421 aattctggct caatggcgtt gatcgtcagg aattactgcg tttagttcgc aaacaagcca
    15481 aaggcgatga gctgactgct gatgagcgca aacaatttaa atacatgcga gcgcgctaca
    15541 agcacctgcg ttttgcgcaa cggctgtatt taaagaagca tcaggccgga tttttattcg
    15601 gcaaaacgac cgtttttctt gggcgttttc aggacggctt tcgtaacggt aagaaaaata
    15661 ttgtgtcgta ttacggcaac ctgttacgga tttatttaag ctctccggtc tggtcgttgg
    15721 tgaactattc attacgccat agccagttag aaagcgtaag cagttttatt gcctatcggc
    15781 aaaagcaaat gcataccctg aaagagatta ttgccaaacc gcgactaacc ggcagggaat
    15841 ttcatgatgt gcgaaaaatc atcagccaac aggtttctta ttacgacacc ttaagatcgc
    15901 ttgatcctga aaataaagag gcgttacaga tttcccgttt cctcgccgcc attaatggcc
    15961 tgatgggaga taaacacgac gacatggtgg cggatgatat ggaaaaccgc cagtcgtatg
    16021 atgcaccgtt ggcgctggat agcgatattc gtcagcgctt agagctgctg atttcgcgtt
    16081 ttccgctata accggagcaa tgatgtcatc tcgtttgatt attgcgctga ttatcatgct
    16141 tttagccccg ggtgtgcagg cacacaattt cgtcactggc aaaacggtta cccctgttta
    16201 tattcaggaa ggcggcgagc tgcttctgaa tagcgacgac gaaattcact accaaaagtg
    16261 gaacagcaca caattggcgg gaaaagtccg cattatccag tatatcgccg ggcgtaagtc
    16321 ggcgaagaaa aagaactcac tgttaattaa agcggttgaa gcggcaaact ttccgcagga
    16381 tcgttttcag cccaccacca ttgtgaacac cgatgatgcc atctttggta ctggctattt
    16441 tgttgttggc aaaatcgaaa aaaataaacg ccgctatccg tgggcgcaat tcgttattga
    16501 cggcaatgga cagggacgcg ttgcctggcg cttgccggag cagagttcaa ccattctggt
    16561 gctgaataaa gccgggcaaa ttcagtgggc aaaggacggc agcctgacgc cggaagaggt
    16621 agatcatgtc attgcgctgg cgcagaaact gatcaatgaa taaaaaattc aaaggattga
    16681 gggcgttagt gagatgaagc tggttgaaca ctacatcatg cgtggtacgc gccgtctggt
    16741 gctgattatc gtcggttttc tgatctttat tttcgccagc tactccgcac agcgttatct
    16801 gaccgaagcg gcaaatggga cattagctct tgatgttgtg ctggatatcg ttttctacaa
    16861 agtgctgatt gcactggaga tgttgttacc tgttggactg tatgtgtcag ttggcgtgac
    16921 gctagggcag atgtataccg attcggaaat taccgctatc tctgcggcgg ggggcagtcc
    16981 gggacgcttg tacaaagccg ttctttatct ggcgataccg ctaagtattt ttgtcaccct
    17041 tctgtcgatg tatggtcgtc cgtgggctta tgcgcagatt tatcaactgg agcagcagtc
    17101 gcagtcggag ctggatgttc gccagttgcg ggcaaagaaa tttaacacta acgataacgg
    17161 acgaatgatc ctttcgcaga cggttgatca ggataataat cgcctgactg acgcgctgat
    17221 ttatacttct actgccaatc gaacccgcat tttccgcgcc cgttcggttg atgtggttga
    17281 cccatcaccc gagaaaccga ccgttatgtt gcacaacggc actgcctatc ttctcgatca
    17341 tcaggggcgt gacgacaacg aacagatcta ccgtaatctg t
//
LOCUS       DAMZET010000088        17201 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.088, whole genome shotgun sequence.
ACCESSION   DAMZET010000088 DAMZET010000000
VERSION     DAMZET010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17201
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.088"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(3..338)
                     /locus_tag="POB34_002939"
                     /pseudo
     CDS             complement(3..338)
                     /locus_tag="POB34_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(493..777)
                     /gene="ppnP"
                     /locus_tag="POB34_002940"
     CDS             complement(493..777)
                     /gene="ppnP"
                     /locus_tag="POB34_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDI5919846.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(849..1526)
                     /locus_tag="POB34_002941"
     CDS             complement(849..1526)
                     /locus_tag="POB34_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HDI5919847.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNRGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(1784..1975)
                     /gene="yaiA"
                     /locus_tag="POB34_002942"
     CDS             complement(1784..1975)
                     /gene="yaiA"
                     /locus_tag="POB34_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HDI5919848.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(2025..2549)
                     /gene="aroL"
                     /locus_tag="POB34_002943"
     CDS             complement(2025..2549)
                     /gene="aroL"
                     /locus_tag="POB34_002943"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HDI5919849.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(2726..3190)
                     /locus_tag="POB34_002944"
     CDS             complement(2726..3190)
                     /locus_tag="POB34_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDI5919850.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAAINPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRGQM"
     gene            3310..4119
                     /gene="proC"
                     /locus_tag="POB34_002945"
     CDS             3310..4119
                     /gene="proC"
                     /locus_tag="POB34_002945"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDI5919851.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(4136..5251)
                     /gene="adrA"
                     /locus_tag="POB34_002946"
                     /gene_synonym="dgcC"
     CDS             complement(4136..5251)
                     /gene="adrA"
                     /locus_tag="POB34_002946"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HDI5919852.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(5353..5673)
                     /gene="psiF"
                     /locus_tag="POB34_002947"
     CDS             complement(5353..5673)
                     /gene="psiF"
                     /locus_tag="POB34_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HDI5919853.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(5792..7207)
                     /gene="phoA"
                     /locus_tag="POB34_002948"
     CDS             complement(5792..7207)
                     /gene="phoA"
                     /locus_tag="POB34_002948"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HDI5919854.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWQGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(7308..7568)
                     /gene="iraP"
                     /locus_tag="POB34_002949"
     CDS             complement(7308..7568)
                     /gene="iraP"
                     /locus_tag="POB34_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HDI5919855.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(7750..7953)
                     /locus_tag="POB34_002950"
     CDS             complement(7750..7953)
                     /locus_tag="POB34_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919856.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            8031..9125
                     /gene="ddlA"
                     /locus_tag="POB34_002951"
     CDS             8031..9125
                     /gene="ddlA"
                     /locus_tag="POB34_002951"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDI5919857.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(9149..9361)
                     /locus_tag="POB34_002952"
     CDS             complement(9149..9361)
                     /locus_tag="POB34_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HDI5919858.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            9621..9929
                     /locus_tag="POB34_002953"
     CDS             9621..9929
                     /locus_tag="POB34_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HDI5919859.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(9988..11082)
                     /gene="yaiW"
                     /locus_tag="POB34_002954"
     CDS             complement(9988..11082)
                     /gene="yaiW"
                     /locus_tag="POB34_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HDI5919860.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVGSVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPPFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(11095..12315)
                     /gene="sbmA"
                     /locus_tag="POB34_002955"
     CDS             complement(11095..12315)
                     /gene="sbmA"
                     /locus_tag="POB34_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HDI5919861.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            12667..13824
                     /gene="ampH"
                     /locus_tag="POB34_002956"
     CDS             12667..13824
                     /gene="ampH"
                     /locus_tag="POB34_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HDI5919862.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKQLVIPAS"
     gene            complement(13825..14448)
                     /gene="iprA"
                     /locus_tag="POB34_002957"
     CDS             complement(13825..14448)
                     /gene="iprA"
                     /locus_tag="POB34_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HDI5919863.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(14536..17201)
                     /locus_tag="POB34_002958"
                     /pseudo
     CDS             complement(14536..17201)
                     /locus_tag="POB34_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         4336 a         4368 c         4066 g         4431 t
ORIGIN      
        1 tgtcattgtt tttcctcaag ccggtgacgc gcttcactgc gaattttccg cgttgccccg
       61 gttttcacta aatcacgtaa ctgcgaaaga tcgggttgtt cgacaaataa gagtaatgat
      121 ggatcctctt ttaaccacgc cgttttcaca tcggcagaca gctgtggatt attaatagcc
      181 agcgaccgat cctgaggctc agtcaatgtc gttaacaaca acgccggggt atgtgcgttg
      241 cgcaatgccg tttggcgcaa atgatcattg cgactttgca gcaatgcacg ccaccattca
      301 tcatctgatt tatcttttat cgtagccagt accgcaaaaa atacatggag atcttcactg
      361 atatttatat caaccttgat gaattatgaa tataccggaa atcgaaaaaa tcgcagcaaa
      421 ggaaatatta ttatgggttt atgagaggat atttcagaaa aatgccctcc cgttcaaggg
      481 gaaggcgagg aattacagat agcgacacag ataagaggtg ggttcggcaa cttgcagatg
      541 aaactcgctg tgaccgggaa cattaaacac cgaaccggct tcatacactt gccagtcggt
      601 cgcgtcaggc agtaacacat tcagcgcgcc actgattacc gtcatctctt ccggctcagc
      661 ggtgctgaag gtgtattcgc cttcaaccat aacacccaca ctggcgcgac cagtgctgct
      721 gctggaaaag ccgatcgatt tcactttgcc ggaaaagtac tcattacttt gaagcataaa
      781 ctggccctgt ctggtagttg aatatctact tcactatagg ggccagaggc gctgactgtc
      841 acgcaaaatt acaccagtaa ttccgcagcc agccgtgcaa tcaatacgtt agacagcaag
      901 acaggaacat cgagctgttt ttgcagtaaa tcgcgatgac gttggtgaaa tcctaaacaa
      961 tccagcatga tgacatcagc cccttttgcc agtaattctt tcccggcatc aatgattttt
     1021 tgttctgaat catgaatggg gttacccaat gaaaataccg gcgatttctg caaaatttgc
     1081 catttttgcg cctgaacggg cagcatctcc tcaaccggaa cgataacccc cacttgatga
     1141 tcttcaacaa tagaggaaac cagcggtggc aatatccgcg acggctcaag aaagatcgta
     1201 ttacgcgcag tcatactact aatgtttgcc gtactcatta atataatgac gtcgtaaccc
     1261 cgattatcga gtacttcaac cacaccttgc aggtcacgct ccactttgcg acgcgaaaca
     1321 tgggccagct ggttgtcatt taataatgtg agaatggtgt cttcgcctgc ttctggcgcg
     1381 tactccgcca tcacttcttc acgacttaac ttccccagca ggctatgatg ggaaatatta
     1441 tcttcgtcaa tgtattccgt caggagcggc aaaacttcct gcataggtac aatgccgatg
     1501 gtcaggatcg ccaacgacgc actcattgtt catccacctt acttttcttt cgtcgttaat
     1561 taccgggcaa gtgtgaagca ccactgctga cacttacttg tgctacaaat gacaaaaagc
     1621 gtagcagcag acgtgcacgg cataatgtaa agattccaaa tgattccagt aatggattgt
     1681 tattgtttaa tattctaatt attagaaaaa catgaattat gaaaaatgtg acgcagatcc
     1741 cttgtagtgg cactcagaat cccttccgag cagtctgatg cgatcactct ttgtcagggt
     1801 cctcatagcg ttttttcgca tccatcgctt cctgagcggt cggatgttca ctgatcaacg
     1861 agtctggttt aggatgatcg gctctgagtt ggtaccaggt taccgtctgt cctggttttc
     1921 ctttttcaat cgtcactata tatgcttcac gaggataagg cggtttcgtt ggcatcgttc
     1981 ttccttattt cacgggatga acgttaagta taggcgctcg aaaatcaaca attgatcgtc
     2041 tgtgccaggg cgctacgaat ttcagaaatc acctggctgg gttcgtttgt tgcgtcgatg
     2101 ataatatgcg caacttcgcg atatagcgca tcgcgttctt ccagcacttc ctgaacttct
     2161 tcgctcagcg gttttcccgt taaggttggc cgtaaatctt cttccggtgc agcttgcagt
     2221 cggttaacca ggactgatac tggcgcacac aaataaacca cgatcccgtt attttgcatg
     2281 aagtgacgat taaattccgt cagaataatg ccgccgcctg tagcgataac ggtggatggc
     2341 gcagttaccg cttccagcgc cgccgtttct ctggcgcgaa atcccgccca ctcttccctt
     2401 tcgacgatct ccgcgaccgt catattgagc tgtgattgca accactgatc ggtatcgaca
     2461 aaccgacggt taagcgaatc ggcaagggcc attccgaccg ttgttttacc acagccccga
     2521 ggcccgatca gaaaaagagg ttgtgtcatc gtgggttttc cccaataggt cgcaatgcgg
     2581 cgaaagccgg tgtcatgaga atagcgatca taccatcaaa ctagtacaat ttcgattgta
     2641 aaggaaaaat tccacttaaa gtgaaaatct caatacaccc cttactatac caataaataa
     2701 tcaagaatga agtgtaaata ataaattaca tttggccgcg actacgttgc acttccagcc
     2761 accatttctc cagctctgca gcaaaggcct ggcggtcacg ttgtgaaaga ctatctggtc
     2821 cgccggtctg gatcccactg gcacgtaagg tatccataaa atcgcgcatc gtcaggcgct
     2881 cacgaatggt cgctggcgtg taacgttcgc cgcgcggatt aatcgccgca gcgcctttct
     2941 cgatggcttc agcagccaaa ggtatatctg cggtgatcac caaatcgccc gcttcacact
     3001 gccggacaat ttcgttatcg gcaacgtcga aacctgccgc gacgcgcagc gtacgaataa
     3061 atcgcgatgg cggcacgcgt aaactctggt ttgctaccag taccagcggc atctgcatac
     3121 gttccgccgc gcgatacaaa atctctttaa ttacattggg acacgcgtcg gcatccaccc
     3181 aaattgtcat aaagtcatcc tttgttgggt aatcctctat tgtgtcgcgc ttttgccttc
     3241 cggcatagtt ctgtttatgc ttctgccagc gattatcaaa acaatgaatt tcacggcagg
     3301 agtgaggcaa tggaaaagaa aatcggtttt attggctgcg gcaatatggg aaaagccatt
     3361 ctcggcggtc tgattgccag cggtcaggtg cttccagggc aaatctgggt atataccccc
     3421 tccccggata aagtcgccgc cctgcatgac cagttcggca tcaacgccgc agaatcggcg
     3481 caagaagtgg cgcaaatcgc cgacatcatt tttgctgccg ttaaacctgg cataatgatt
     3541 aaagtgctta gcgaaatcac ctccagcctg aataaagact ctctggtcgt ttctattgct
     3601 gcaggtgtca cgctcgacca gcttgcccgc gcgctgggcc atgaccggaa aattatccgc
     3661 gccatgccga acactcccgc gctggttaat gccgggatga cctccgtaac gccaaacgcg
     3721 ctggtaaccc ccgaagatac cgctgatgtg ctgaatattt tccgctgctt tggcgaagcg
     3781 gaagtaattg ctgagccgat gatccacccg gtggtcggtg tgagcggttc ttcgccagcc
     3841 tacgtattta tgtttatcga agcgatggcc gacgccgccg tgctgggcgg gatgccacgc
     3901 gcccaggcgt ataaatttgc cgctcaggcg gtaatgggtt ccgcaaaaat ggtgctggaa
     3961 acgggagaac atccgggggc actgaaagat atggtctgct caccgggagg caccaccatt
     4021 gaagcggtac gcgtactgga agagaaaggc ttccgtgccg cagtgatcga agcgatgacg
     4081 aagtgtatgg aaaaatcaga aaaactcagc aaatcctgat gatttttgcc ggacgtcagg
     4141 ccgccacttc ggtgcggtta cgtccggctt tctttgcttt gtaaagcgcc aaatctgccg
     4201 atttcaacca ctcgcgatag tgactcattt gtgggttcag cggcgcaacc cccacactaa
     4261 tccgtaaagt tacctgtggc gtattcggca agcgtaacgt gtttagccct tcatgcaccc
     4321 gtaacatggc ggtaatggcg ctctcagctg gcgtaccgga catgattacc gcaaactcat
     4381 cgccgccaaa ccgaccaatc acatcgctac cgcgcagggt aatttgtaac tgtcgggtaa
     4441 gcgccacaat cgcttcatcg cccacatcat ggccccaggt atcgttgatg ctcttgaaat
     4501 ggtcgatatc gataatcagt aatgtcgcat cgcgattatg ccgccgacag ttatcaaatt
     4561 cattgcgtaa catagtttcc caatgacgtc ggttatacac gcccgtcata ccgtcgcggg
     4621 tactcatgac ctgcaacctg cgtttatgtt ccgccagttt agtcgccgtc tggtagctga
     4681 cccagccaaa cagcagagga taaatgacga taatgggaag ggagagccac cattccagcg
     4741 gcgcactatt gaacgacacg gtgatgcccg tcagttcgag ggtgacaagg caggaaacca
     4801 ccatcaacac cagacccgcg acaaacagac gggggccgcc tgcccccatc aaattcagac
     4861 acataatcat caacatcgcg gtggaaggca gcacgtttac gcccattacg cctacccaca
     4921 ttcccgctaa tactgcatcg gtttttaagt tgtaaatttc ccggctaagc ggatcgacgg
     4981 ccctgctcgc tatctgccag gctaaatgcg gccagacgaa cgcccagccg accaacacca
     5041 gccaccacca gcccggcggc gggtgcgaaa ccagcgttga agcaatcggt aagaacatgc
     5101 cagccaggcc aaccgcacgg ggtagtctga cgcgacgggc gaagcgcagc ccggaccgct
     5161 gatgttcgtt ttgaggagtt aaaggaggtt cctccccatg cgccgccgct tttttgaaaa
     5221 agttttcatc attcattatt tttgggaaca ttcagaaaca attttcccaa attatagaga
     5281 cggggctaaa ggcagaagta tgaaatttcg ggtgagccac tttcgtagct cacccgttag
     5341 cactatcagg tatcaggcgg ctttcttgag gcaggcactc ataaacttat tacgatcatc
     5401 acctttcaga gattgttgcg ttgcttgatt attacattcg cgcatctttt gctgctgtgg
     5461 cgtcaaactt ttttcaccag gcgcagactt gctgttcttc aggcaatcac tcatgtaggt
     5521 cttacgagca tcccctttca acgcctgcgc cgtcgcctgc tgattacagg aggtcatacg
     5581 ctgttgttgc ggggttaagg ttctctcggc agcgccgacg gtggttaaaa aaaccagtcc
     5641 gaaaagcaag gtaaccagta atgttatttt catagcacca tccctcttca tgttttaacc
     5701 atgagcgttt gcgcccgtga tctgccatta agtctggttg ctaacagcaa aaaaaccacc
     5761 cgacagcgaa aattcaccgc cgggcgcggt tttatttcag ccccagggcg gctttcatgg
     5821 tgtagaagag atcggtctgg tcggtcagtc caacgacatt ggcggcatgt gggccatacg
     5881 ccgcaatacg cagctgacta ccggtatgtt cttgtgaatc ctcttcggag ttcccgtaac
     5941 tcatcaccat cactgcgcca tctttggtat ttagcgcctg ggtgaggccc ggcgctttgg
     6001 tgtccggcgc aacaatctgg ctggcgtggg cgtgatcagc ggtgactatg accagcgtgt
     6061 tgccatcctt tttagcgaat tccagcgccc gttgtacggc ttcgtcgaga tcgaccgtct
     6121 cgccaatttg cccacaagga ttcgcagcgt gatcctgttt atcgattgat gcaccttcaa
     6181 cttgcaggaa aaagcctttc tcatttttac tcaacaattc aatggcttta tcagtcatct
     6241 gcgccagggt cggtacgctg tcattacgtt gcggattagg cgtacaggta actgcgggct
     6301 tgtcgatatt gccgtggtac gttgctttcg gtccttgcca gcgcactggc atattgccgt
     6361 cagcaaacag tcctagcagg ggtttttgct ggttcgcttc cgtcaccgca ttcagtgagg
     6421 cagcatcgct caccaactga taaccacgcg cctgtgcctg ttcacgcagc gtttttccct
     6481 gccattcacc ggcggttgcc gtttcagcaa aggtttttgc gccgccgcca agcgtaacat
     6541 cggcacgagc gttaagcagc tgttcggtaa tcgatccttt tccgcctttt tctagcgcgt
     6601 tacccggaca tttttcactg gtcgcgctgg gaccgtagca tttgcgcgag gtcacatgcg
     6661 ccaccagcgc agcgggcgtg gcatcctgca actctgcggt agaaacgtta ccggtcgcca
     6721 gacctgcggc ttttgccatt tccagaatcg ttgggtgatc tttttcgtga atatcgacgc
     6781 ccagcgcgcc gttataggtt ttgacaccgg ttgaccaggc ggttgctgac gcagccgaat
     6841 cggtgacgta gtccggtttg ccggtttttt tattcagcgc atagtgagtg tattgcccgg
     6901 taagcggtaa tgcatcgata cctttaaaaa agccgcccgc accttcggca taattacgtg
     6961 cggcagtaat ttccgaatcc cccatcccat cgccaatcag caaaataata ttttttgcag
     7021 gtttatcgct aagagaatca cgcagagcgg cggtctgatc acccgttaag cggcgagcac
     7081 cgccgggtgc agtaatatcg ccctgagcag cccggttttc cagaacaggc atttctggtg
     7141 tccgggcttt tgtcacaggg gtaaacagta acggtaagag tgccagtgca atagtgcttt
     7201 gtttcacttt attttctcca tgtacaaata cattaaaaaa taaaaacaaa gcgactataa
     7261 gtctcggccg tgacaacttt atgacagctg ttgaaaagat taacttttta ctgacgagga
     7321 tgcttcaata acttctttac gtaatcgcgc agcagctccg tatcgtcgtc aggaatgctg
     7381 gcatcgggct ttacctcgta cagcgccccc tctacctgat caatcaaccg ctgttggtca
     7441 ttttgcgcca tattgcgaag cattgcagtg acgataatct ccaaagcttc tacctgcgca
     7501 cacagttctt tcgactcttc ttctttttgg gcaagcttaa ataacaactc agcaatgaga
     7561 tttttcatgt ctgtatttcc ttatccaaag tatggagaag ttaacattat gtttcacaac
     7621 ttcatagtgt ctggaagtat tctttttatg acaaaggaaa tactttgcgc aaatattttt
     7681 tgtttgatta ccagcgaaac gtttctctta tttaaataac tacccacaca aaatataggc
     7741 ttttaattat caacctttat tataaataaa agttatgaaa tatattctct tcgttaactt
     7801 ataattaaga gaaaaaaact caccgcattg ccatttaagc caggaataaa ttagcttccc
     7861 gttcatcatt tttgtgatct tgcgcacatc tttgagataa cgcactttct tcaccagtac
     7921 gttgcacgct ttgtcagcag aagattgcaa caatctttca gaaggccgcg aatcccccgt
     7981 tttgtcgtgg taatgtatgc actttgtgtt agacgggata ggtttttaag atggaaaaac
     8041 tgcgggtagg aatcgttttt ggtggtaaat cagcggaaca tgaagtgtct ctgcaatcgg
     8101 caaaaaacat tgtcgatgcc attgataaaa gtcgcttcga cgttgtgctg ctgggcattg
     8161 ataaacaagg gcaatggcac gtcagcgatg ccagcaatta tctgctaaat gcagacgatc
     8221 ctgcccatat tgcgttgcgc ccttcggcga ccagccttgc gcaggtgcca ggtaaacatg
     8281 agcatcagct tatcgacgcg caaaacggtc agccgttgcc gacggtggat gtcattttcc
     8341 cgattgtcca cggtacgctg ggcgaagatg gttccttgca gggaatgctg cgggtcgcca
     8401 atttaccgtt tgtaggttct gatgttctgg cttcagcagc ctgtatggat aaagatgtca
     8461 ccaaacgtct gctacgcgat gccgggctga acattgcgcc atttattacc ctgacgcgcg
     8521 ctaatcgtca caacatcagt tttgccgaag tggagtctaa actggggtta ccactgtttg
     8581 taaaaccggc taatcagggc tcttctgttg gtgtcagcaa agtaaccagt gaagaacagt
     8641 acgcaattgc cgtcgatctg gcgttcgagt tcgatcataa agtgatcgtt gagcaaggga
     8701 tcaaaggtcg tgagatcgaa tgcgcagttc tgggcaacga caatccgcaa gccagcacct
     8761 gtggcgagat cgtactcacc agcgatttct atgcctacga caccaagtac attgacgaag
     8821 atggcgcgaa agtggtagtt ccggcagcca ttgcaccaga gatcaacgat aagatccggg
     8881 cgattgccgt tcaggcttat cagacgttgg gatgcgcagg catggcgcgt gtagacgtgt
     8941 ttttaacccc agagaacgaa gtggtgatca acgagatcaa cacactgcct ggcttcacca
     9001 acatcagtat gtatccgaag ctgtggcaag ccagcggtct gggttacacc gatctgatca
     9061 cacgtttgat tgagctggcg ctggagcgtc acgctgcgga taacgcattg aaaaccacaa
     9121 tgtaatattt taacgcccga taatattctt attgggcgtt ttcagtttct tcatcgcgac
     9181 gacgaataat caaccccgcc agccagaaac taatcaccca cgtcaccaga ccgacggcat
     9241 aggtctgcca accttttgct tcaaatccca gtaaccccac cacgccatta agaatgaata
     9301 taaggccaat ggcgaacgca tagtagtgcc agtcacggcg gatttttaca ggcagattca
     9361 tggcagctcc gttaacgaaa aacaacatcc ttgcatattc tgtaacaacc gtctgtggtg
     9421 tagacaacac tttcgcaatt gttaataaat ttaacttaaa gttgctgttt tgtgactcgc
     9481 aacgaagatt cacgcttcgg gaatattccc aacatcaatt taccatgatt ctgatattga
     9541 caatctaaat gacctgtcag acttgccata agccgctggt atcacaccag aagagaagca
     9601 gatgacttcc ggaggttttt atggctgatt tcaccctgtc aaaatcgctg tttagcggaa
     9661 aatatcgcaa tgcctcttca acgcctggca acattgccta tgcgttgttt gtgctgtttt
     9721 gcttttgggc tggggcgcaa ttgctgaacc tgttagtgca tgcgcccggc gtctatgagc
     9781 gtttaatgca agtccaggaa acaggtcgcc cacgggtgga aattggttta ggtgtcggca
     9841 ccattttcgg gctgatcccg tttttagtag gctgcctcat ttttgcagtg gtggcgctat
     9901 ggctgcactg gcgacatcgc cgccagtaag ttttacgttg tcagatgcgg cctgaaacgc
     9961 cttatctggc atgacattcc tgtttgatca ctgtttcata caacgcgccc gccgttcgtc
    10021 tacgcgcttc gcaaaccagg ccgtagtcag gttgcgggtg attttcgggc tttccagttg
    10081 aatgccaggc aacatttcgc ggggtagtga tttaccggtt ttcgcttcgg caagttgata
    10141 aactttcttg tacagcgccg tctcctcaaa agagaagctg tcacctttct ctagctgacg
    10201 acgaatttcg ctgtcgctca tccccagttt tgccgccagt ttgcgcgttg ccagttctgt
    10261 tttcccgggt tctttactgt cgtaacgaat taaatcaccg tccagtgcca gcttcacgcc
    10321 gctggcctta ctgaccgcgt tctgaaacgc ggcattacgg ctggcgtacc agccagcgtt
    10381 aaaatcagca aaacggtata tcggtgcact ataactggcg ggataattca gtaaatggta
    10441 agtaccaaac cacaacccgc cgcggcgact aaagacttcc tgacgaactg taccgtccat
    10501 tttccacgga taccctttgg tatgctgttc ggcaaaagca atgctgacct gcatcggccc
    10561 accggtgcgc accggattaa gcgaaccaaa cagcgtctgc cccattggca ccatgctgat
    10621 cagatcgtca aaaatcgcgc tcaattgctt ttctgtacgc accgaatcta accgttcgct
    10681 ataacttttg ccgtttggcg atttgatttt tagcgcggta tgaaccagaa aaggaggaat
    10741 gtgcatccgt tcagcacgac ggtcaatctc ttgccaggcg atcttgctta agcccggaac
    10801 ggccggatct gcctgataat ttgactcctg ctgcgccacc gccagcaccg agcagacgtt
    10861 ttccagcgtc ggtgccagcc cctgactttc aaaggtggtg gcgagatctt tcgcccaggc
    10921 atcgcggtct ttcacgctcg caggcatctt ctgccgcacg acgctcccta catctatcgc
    10981 tttttcgccc tttttcaacg gttgcggagc ctgactacta caccccgcta aaaccagaac
    11041 cgccaacaat gacagcgaag atagaggatt gacgcgcgac attacttctc ctttttagct
    11101 caaggtatgg gttacttcct gaattttgtc accatccagc tcatgttcaa agctgcgcag
    11161 acgtttgtag atagacatca actcaaccag tgtggtccat gagttaatca ggtactggaa
    11221 agcaccgcga acctgaccaa aaacgttggt aatctgcgtc atcaggccga gcgtaatcgt
    11281 accggcaaca attgacggaa acagcaagaa caaaccgaaa acgttatcga cctgcaaata
    11341 gagaatgcgg gcgatgttga aatacatata gtgaaaatag aggcgaaaat agtttttccg
    11401 tacggcgcta aacagctcgc gtaccgtagg cggcgtcgcg cgcgtggcat catcttcacc
    11461 ataaaccagc tctttacggt aggcagcctc tacacgctgg tttttaaact ccagccccgg
    11521 cagtttgatc cctaccactg ccagcaatcc ggtccccatc agcgaccaga cgatagcggc
    11581 aatcaccaga ccatacggaa tgtgcccgat aatcggcagc tccggcacat gcgcggagag
    11641 cgttaccagt accggcagga aggcgattaa cgtcatgatg gcgttgataa aactgacgcc
    11701 catattctcc agcgttgaag caaaacgcat ggtgtcttcc tgcacacgct gtgcggcacc
    11761 ttcgatatga cgcagttgtt gccagttcgc catgtaatat tcgttcatcg ccgtacgcca
    11821 gcggaacacg tagtgactga caaagaagtt attcagcaca ctgatcacta ccgcgatcag
    11881 cgcaatcccc agaaagacgc ccacttcgcg gtaaaattgt tcgatggtga ctttatgcgg
    11941 cgaacttagc gcggtttgaa tcagatcata gaacggcgca taccaggcgt tgacggcaac
    12001 cccgacttcc accaaaaacc aggtgacgaa gataatcagc gcagtaccga gtattgacca
    12061 gtattgccaa cgatgcgggc tgtagataaa ccagaacaat gcaaaaagtc ctacgcaaac
    12121 aatgtagtaa gcgtaaaaaa tcaggaaatc caacgaccag aaacgcgcgg cgctaatcgg
    12181 gatctgcccg gaagcgccgg tgatacgcgc cacccagtca cccccaccgg cttgccagaa
    12241 gataacggca atcaatgccc aaacaaaggc cgagagaaaa aacgttcccg gctttgggaa
    12301 aaaagactta aacatatgca ctcctgctaa cttcttatcg ttttaacaac tgctgtgtat
    12361 taaccgcatg accgctacaa agcgtcagcc aaatgaaacc gcccattgtg acccatagca
    12421 aggcgcttag ttcgccagcc tgaccataag aattgtttcg acgggtttca caaaccaaag
    12481 attgtttaca taagcggatt aacacgcgca ggatcacaaa tgcaggcatt ggtgagcagg
    12541 aaaaaaatcg tgtttacgcg ccgttacgtt tttaacacta ttattgcact aattcgccct
    12601 ttgcaatcca tcaatgagta gtataaatac gctcagttac cttcattcaa tttatggaca
    12661 ccaccgttga aacgtagtct gcttttttct gccgtgctgt gtgcggcgtc attgacctcc
    12721 gtccatgcgg cgcagccgat cactgaaccg gagtttgcct ctgatattgt cgatcgttat
    12781 gccgatcata ttttttacgg cagcggtgcc acggggatgg cgctggttgt tatcgacggt
    12841 aaccagcgcg tctttcgcag ttatggcgaa acgcgacctg gtaataacgt tcgcccgcag
    12901 ctggattcgg tcgtgcgtat tgcttccctc accaagctaa tgaccagtga gatgctggtg
    12961 aaattgctcg accaggggac cgtgaagtta aacgatccgc taagtaaata tgctccgcca
    13021 ggcgcacgcg tgccaaccta caatgggacg cccattacgc tggtcaatct ggcaacccat
    13081 accagcgccc tgccccgtga acagcccggt ggcgcggcac atcgtcccgt gtttgtctgg
    13141 ccaacgcgcg agcaacgctg gaaatacctt tctacggcga agctgaaagc cgcgccaggc
    13201 agtcaggcgg cgtactctaa ccttgcgttc gacctgctgg cagatgcact ggcgaatgcg
    13261 tccggcaagc cttataccca gttgtttgaa gagcagatta cccgtccatt ggggatgaaa
    13321 gacaccacct acaccccctc accggatcag tgccgccgtt taatggttgc tgagcgcggt
    13381 gccagtccgt gcaataacac gctggcggca attggcagcg gcggcgtcta ttccacgcct
    13441 ggcgatatga tgcgctggat gcagcagtat ttgtcatctg atttttatca gcgcagtaac
    13501 caggcagatc gcatgcagac gttgatttat cagcgcgcgc agtttaccaa agtgattggc
    13561 atggatgtcc ccggcaaagc ggatgcgctt ggtttaggct gggtatatat ggccccgaaa
    13621 gagggtcgtc cggggattat tcagaagaca ggcggtggtg gcggtttcat tacctatatg
    13681 gcgatgatcc cgcagaagaa tattggcgcg tttgtggtgg tcactcgttc gccgttgacg
    13741 cgctttaaaa atatgagcga tggcatcaat gatctggtga ccgagctaag cgggaataaa
    13801 cagttggtta tccccgcatc ctgattaata ctctgaaggc aaacggttga tagcgaccag
    13861 tttgccttta ttcatttcga tatagccgcc tttacgcaaa gcagcgagaa cttctgcgac
    13921 gacagaacgc gatatgcgtg tacgttgatg gatataattc atcacaccaa tacgtgatcg
    13981 caattcttca ttccagtcaa tcattgataa taatgttgcg cggatttgtt cgtaggaatt
    14041 atgcccaatg agctgcacgt cacgtaattc cagaattcta ttttgccagg ctaaccagta
    14101 aaaagcgtct cgccagagtt gattttgttc aataagcgta atggtttgtt tggctggtag
    14161 atgatatccc gtacaatttc cttctgatat taatttgtat ggtatatcgt ttttcattaa
    14221 accatcagcc agccccataa tataaggagc ctgggtaata ccgataagta cgttttcttc
    14281 tctacgcaga gagataactc cctctaaaat aacgaaagta tcttcgttat tgacatcact
    14341 ggaaaatata acttgctttt cattattaaa ctcgaagcgc gtaccgtatc tggacaaaca
    14401 tttatcgagc ttaccaaatt cctgaagagg tttaactaca gataacattt gcgcgtcctt
    14461 tgcagtaatg cccgtcaaat ccttgacggg cattatttag attaaattac cagaatttct
    14521 tcggagtgaa gaatattacc aggtatattt aacacccacg ttcgcggacc agtcttgatc
    14581 tacgtcacca ccaccgaggt agttagcatc ggtataggcg ctaaagttct tggtgaagct
    14641 gaactgagta cccagaccaa cacgtaccgc agacccttca gtaccgttat cgatggaatc
    14701 accgttcaca tcgttatcgt tgttagagtc gtcgtagacg taagccagtt tgaagtacgg
    14761 agtcagagcc tgatcttcgc tgtaggtgaa ggtataacct gcatctacac ccagttcata
    14821 acgcatgctg tcgtaagact gaccgtcaac tttcatgtcg ttgctcagct agtagtcatc
    14881 accagactgg aacagaccag aaacgctgcc gtaaggagtc acgtaaccag catcacccag
    14941 tttgaagtcg taaccggctt tcaagccgaa gccccaagcg tcggagttgg tgctaccgtc
    15001 aacgtaagta ccgttgctca tggttgcaga caggtcgttg ttgaagtgag agtagctcaa
    15061 gctaccatca acaaagacgt tgttcgcgaa gtgagcagaa gagtagatgt aggcagtctg
    15121 gctgtcttga tctacctgac cagaacggtc attcatgtca cctttagcga agcctgcagc
    15181 cgcaccgacg atccacttag cgttgttacc gtcaactttg gtatcaacac cgaccatgat
    15241 gccgttaaca tcctgatcat agttgatggt gccgttgtcg ccgttgaagt taccaccgaa
    15301 gtagcttacc catgcgccgc cgttatcagc caggccatga cgagagttgg tcagacgagt
    15361 accaacggtg tcttgttcca ggttccagat attggtgttc gcagacggga tgctcagcgc
    15421 catgttagcg tagtcggtca gctccatctg ttgcagaaca acggtgttac cgcgctgttc
    15481 agcctgatag gtgtatgcac ccaggtcagc tttgttagca gcagagaagg tcgcgttggt
    15541 gttagcgtcg tttacccgaa taagttcttt atctttgtaa tcagcgatag tgccagcgcc
    15601 agttgcgttg tcgatacgaa ctttgtagtt accggttgca gcaacaacgt tatcaacaga
    15661 gctgttatcc agttcagtac cgttgtctac gtcgccgtta ccattgatag tcaggtgacc
    15721 atcagagttc atagcaacaa taccgtaacc gtagttagac ttggaagtat cccacgtacg
    15781 gtcgttcacc agatcagcgt tcagaacgta atcgctgctg ttgatgtcga acacaccaga
    15841 aattacgttg ccattatggt cagcaatgtt gccgctggtc agttccagtt tatcagtacg
    15901 gaaagcttca gtatcgaact gatcaacatt cacatccagc acaccaccgt tagtcacagt
    15961 gatagtattg gtatacagat tagaataatt attccaaccg gtagattcgg ttaaattaac
    16021 ggtactgtag ctatcaacag tcaggtggtc tgtagcaacc acgccatttg caccaatatt
    16081 cagtgaagaa ccgttggtta aaccaatggt atctgatacc agagaggaat cagtaacatt
    16141 tacctgagag ccattattta cagctaatgt atcaattact gaactattaa cggtattcca
    16201 ttctgaacta ttgttcagtg ctacgttaaa tacgccgctc tggaatactt tgttgtcaat
    16261 aatacggccc caatcattag cttcgatggt ggcaccaggg gtcaggctgt tcggcgcaac
    16321 gtcatacata tctgccggag caattacttc agcactcatg tttgctgaac caacccactt
    16381 actaccgtta ttcagagtaa tattcagttc gtcaacgttc tgagcgtcat ctacccaacc
    16441 gccgttggta tcaaggacgc cgtcgccgtt gctgtcatga ccggtggaat agaagaatac
    16501 gccatcgtta ttccagtgac ttgtaaattc gacatcaccc aacagagttg aatggtcaaa
    16561 gtttacattg tttttcatgg aataatttga gccagcattg tcagtgaatg acagtgctac
    16621 gtcattctgg ccaccattac cagcgtaatc acttggttca gcagatttac cgaagaagcc
    16681 atcactttct tccagcggag tctgtgatcc tgaagttaca gtggaattag acacattgat
    16741 gttgttgtcc cactgatgat attcgctagt ataagttgtg ttgcttgtat tgccttcaga
    16801 cagcgcaatg ccagctacac gggagttgtt ggtaatattg atattgcttt caacatcaag
    16861 atttactgca acacccagag cggtatagaa cgtttcattg gttgacttag atttatcacc
    16921 atcgagatgt gtgtcagtga agtaaagagc ttcatagtca tcatcaatgg tggaattgga
    16981 tacgttcaga gtgaatacgt caccatcgta ccaattgtag tcaagatcag tagtagttcc
    17041 gttattgaaa gtatagccat tataaccttc aggcgtaatg ctgtaataac caaatggcag
    17101 ttcagaagtg attgacccat gaattactga attgctgata tctacctggt taacagaacc
    17161 gctagcgtaa tcatgattca agtaataagt agaaatggta c
//
LOCUS       DAMZET010000089        16797 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.089, whole genome shotgun sequence.
ACCESSION   DAMZET010000089 DAMZET010000000
VERSION     DAMZET010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.089"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            139..828
                     /gene="pepE"
                     /locus_tag="POB34_002959"
     CDS             139..828
                     /gene="pepE"
                     /locus_tag="POB34_002959"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HDI5919864.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(919..2550)
                     /locus_tag="POB34_002960"
     CDS             complement(919..2550)
                     /locus_tag="POB34_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDI5919865.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(2770..6453)
                     /gene="metH"
                     /locus_tag="POB34_002961"
     CDS             complement(2770..6453)
                     /gene="metH"
                     /locus_tag="POB34_002961"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDI5919866.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            6653..7477
                     /gene="iclR"
                     /locus_tag="POB34_002962"
     CDS             6653..7477
                     /gene="iclR"
                     /locus_tag="POB34_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HDI5919867.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSAHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            7794..9368
                     /locus_tag="POB34_002963"
     CDS             7794..9368
                     /locus_tag="POB34_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HDI5919868.1"
                     /translation="MITRIPRSSFSANINNTAQTNEHQTLSELFYKELEDKFSGKELA
                     TPLLKSFSENCRQNGRHIFSNKDFVIKFSTSVLQADKKEITIINKNENTTLTQTIAPI
                     FEEYLMEILPQRSDTLDKQELNLKSDRKEKELPRIKLNGQCYFPGRPQNRIVCRHIAA
                     QYINDIYQNVDYKPHQDDYSSAEKFLTHFNKKCKNQTLALISSRPEGRCVAACGDFGL
                     VMKAYFDKMESNGISVMAAILLVDNHALTVRLRIKNTTEGCTHYVVSVYDPNVTNDKI
                     RIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHIINQSVAIIPALPKEQLLMLKGSVDE
                     ITPPLSPATMNLLMAIGQNHQLTQLMIQLQKMPELHRTEMLTAYNSGHMNVINTIFNA
                     LPTLFNTFKFDKKNMKPLLLANNSNEYPGLFSAIQHKQQNVVETVYLALSNHARLFGF
                     TAEDIMDFWQHKAPQKYSAFELAFELGHRVIAELILNTLNKMAESFGFTDNPRYIAEK
                     NYMEALLKKASPHTVR"
     gene            complement(9337..11073)
                     /gene="aceK"
                     /locus_tag="POB34_002964"
     CDS             complement(9337..11073)
                     /gene="aceK"
                     /locus_tag="POB34_002964"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HDI5919869.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(11354..12658)
                     /gene="aceA"
                     /locus_tag="POB34_002965"
     CDS             complement(11354..12658)
                     /gene="aceA"
                     /locus_tag="POB34_002965"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HDI5919870.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(12688..14289)
                     /gene="aceB"
                     /locus_tag="POB34_002966"
     CDS             complement(12688..14289)
                     /gene="aceB"
                     /locus_tag="POB34_002966"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HDI5919871.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISEIASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(14558..15487)
                     /gene="metA"
                     /locus_tag="POB34_002967"
     CDS             complement(14558..15487)
                     /gene="metA"
                     /locus_tag="POB34_002967"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDI5919872.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            15644..16087
                     /locus_tag="POB34_002968"
     CDS             15644..16087
                     /locus_tag="POB34_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDI5919873.1"
                     /translation="MVISIRRSRHEEGEGLVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGA"
     gene            complement(16150..16533)
                     /locus_tag="POB34_002969"
     CDS             complement(16150..16533)
                     /locus_tag="POB34_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919874.1"
                     /translation="MSVLYIQISRNKITVRDLESKREVSGDAAFSNQRLLIANFFVAE
                     KVLHDLVLQLHPRSTWHSFLPAKRMDIVVSALEMNEGGLSQVEERILHEVVAGATLMK
                     YRQFHIHAQSVVLSDSAVMAMLKQK"
     gene            complement(16610..16725)
                     /gene="rrf"
                     /locus_tag="POB34_002970"
     rRNA            complement(16610..16725)
                     /gene="rrf"
                     /locus_tag="POB34_002970"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         4113 a         4371 c         4217 g         4096 t
ORIGIN      
        1 gtagcgcatc aggcaatgct gtgtttgtca tcagtctcag cgcccggtgc aatacgtgac
       61 atcgtgagcc atgtcagtaa gtggttaaat tcgcagtggt agcatgcctc ccgtgatttc
      121 ttactaactg gagaagtaat ggaactgctt ttattgagta actcgacgct gccgggtaaa
      181 gcctggctgg aacatgcgct gccgctgatt gctgaacagt tgcagggtcg ccgttcagcg
      241 gtatttattc ctttcgctgg cgtaacgcag acctgggatg attacacagc gaaaacggct
      301 gcggttctcg ctccgctggg tgtatctgta accggtattc atagcgttgt cgatcccgtt
      361 gccgcgattg aaaatgctga gatcgtgatt gtcgggggcg ggaatacttt ccagttgctg
      421 aaacagtgcc gcgagcgcgg gctgctggca ccaattactg acgtggttaa acgtggtgcg
      481 ctgtatattg gctggagcgc aggcgctaac cttgcttgcc caaccattcg taccaccaac
      541 gatatgccga ttgtcgatcc gcaaggtttc gatgcgctaa atctgttccc gctgcaaatc
      601 aacccgcact tcaccaacgc gctgccggaa ggccataaag gtgaaacccg tgagcagcgt
      661 attcgcgaac tgctggtcgt cgcgccagaa ctgacgatta ttggtctacc ggaaggtaac
      721 tggatcacag tgagtaaagg tcacgctacg ctgggtggcc cgaacaccac ttatgtgttt
      781 aaggctggtg aagaagcggt gccgctagaa gctggtcacc gtttttaagt aaattgccgg
      841 atgcatgatc cggcatcttg ttcatagtgc ctgatgcgac gcttgacgcg tcttatcagg
      901 cctacaggat tcacaatatt aatactcgtc ccgtccttca tcttcatccg gctgttccag
      961 cacactgtaa gccaccgaac aaaacagcga gttcaggcgc tgcatatctc ccagtaagcc
     1021 taaatgtagc gaactggttt caatgctctg cacgttttgc tgatgcaggc gatcgacgtg
     1081 ggcgtgggaa tagcggcgat taagaatgcg aaaacgatgt ttgctgcgac gcaaacgacg
     1141 agcgctggtg acatcgccag agaagaacac cgacattgcc agctttaaat tactgagcaa
     1201 ttgctcatag agcgcatcca gttccttcaa cccatcaagc gaaaatgccc gccgtgctgc
     1261 cagcgattta tcggcaattt cgctgcccat gcgctcgacg atatcggaag cctgttcaag
     1321 gttgagcgac atttcaataa tctccgccca gcgacgcgac tcttcttctg ccagttcctc
     1381 ttttggcatc cgcgccagat acagcttaat ggcggtatag agaacgttga tatcatctgc
     1441 cagcttacgc agctctttct cctgccgtgg ctcgccgtgc atcactttat tcagcccttc
     1501 catcatctgt tccatggcgt cgccaatgcg cagggtttcg cgcgcggcgt tcgccagagc
     1561 aagcgtgggc gtatccagcg cgctgacatc cagatgttta ggccgtagct gggtatccag
     1621 ttccggttca tcgcgaataa tcgttttgca aaaccgtgcc atcgggtcaa caaatggcag
     1681 catgaccagg caacgtacaa ggttgtagaa gacgtggaaa tagatcacca gttccgcttt
     1741 tggcaatgac agcttcccca ttgtctctgc cagcaaatgg acaaacggca ggataatcag
     1801 gctacccacc agcttaaaca gcagactacc cagcgcgaca cggcgggctg cggcattggc
     1861 ggcactgttg ttgagcatcg ccagcaggcc ggaaccgagg ttagcaccaa tcaccagaca
     1921 gagcgccacg gggaaggaga taatgcctgc ggcggtcaga gtcgcagtca gcagtacagc
     1981 agcaaggctg gagtagctga taatggcgaa catcgcgcca atcagcgcat ccagcagaat
     2041 atcgccggtc agcgaggcaa agatcacctg aacgccgttt gcctgggtga tcggcgttac
     2101 ggcctgcaca atcaactcca gcgccagtaa aatcagccca agaccaatac cgacgcggcc
     2161 cagttgcccg gcgcgtgact gtttgcgtcc gaggaaaaaa atcacgccga taaaaataag
     2221 taacggtgac agccaggata agtcgaaggt gagaatacgc gccattagcg ccgtcccgac
     2281 atctgccccc agcacaatga ccagagccgg tgcgagggct accagatcct gagcgacaaa
     2341 cgaggtcacc agcatggtgg tggcattact gctctgcacc agtgcggtaa cgccgatccc
     2401 cgcgcaaaag gcgagcggct ttttttcgac gctccggcta aggacagtac gcaaacgcgc
     2461 gccgaagacg cgcattacgc cggttcgaac aatatgagtc ccccagacca gcagggcgac
     2521 ggcagaaagc aggtgaagca gcgttaacac gagttcaggt tctccttatc gttatatgtt
     2581 cctgaagcaa ttgaatgacc aaaggaccgg aaacacgctg actggcatct ttactgtgcc
     2641 ttttgacatt cgtttttccg gctaagatta ttccagcctg tttccagtat aagtgtttca
     2701 tattaataaa gagacagggc actcattgag cgccctgttt gttgtaagga aaagtgacag
     2761 atttgtgaat cagtccgcgt cataccccag attcggtgcc agccagcgct caacatcgga
     2821 gacgctcata cctttacggc gggcatagtc ttcaacctga tcgcgctgaa tttgcgccac
     2881 cgcgtagtac ttgctgtccg ggtggctgaa ataccaaccc gaaaccgatg caccgggcca
     2941 catggcgaaa gattctgtga gtttcatgcc ggtatgtttt tccacttcca gcagctccca
     3001 gatggtggct ttttccgtat gttccgggca ggccgggtag cccggtgccg gacggatgcc
     3061 ctggtagttt tcgcggatca gttcttcgtt gctgaggttc tcgttcggcg catagcccca
     3121 gtagacttta cgcacacgct catggagata ctccgcaaag gcttcggcta aacggtcggc
     3181 aagcgctttc accatgattt tgttgtaatc atcgtgctgc gcttcaaagg catcagccag
     3241 tgcgtcctct tccagcccgc cagtcacggc aaatgcgccg atgtaatctg ctttaccaga
     3301 aagcttcggc gcaacgaagt cagcgagaca gtagttagcg aatcccgttt tttcggtctg
     3361 ttgacgcaga tggtggctga cgttgatcac atgggtgcgc gtttcgtcac ggtagatttc
     3421 aatgtcatcg cccacacggt ttgccgggaa caggcccacc acgccacgcg gattcagcgt
     3481 tttctcggcg cttaatttat ccagcatgtc gttggcgtct ttaaacagcc gctgcgcctc
     3541 aacgcccacc acttcatctt ccagaatgcg cggatacttc ccggccagcg accaggtcat
     3601 aaagaacggt gtccagtcga tgtaattacg cagagtttcg atgctggctt caacttcctg
     3661 cacgccgaga cggtgcgcca ccggcggcgt gtaagcctgc cagtcaaaag cgaaatcgtt
     3721 atcgcgcgcc gcttccagcg tgaccggtgg tgtgcgcggt ttcttacgcc cgtgctgaat
     3781 acgtacggtt tcgtactcct tgcgggtacg agcgacaaaa tcatcacgct gagtatcgga
     3841 aagcagcgcc gccaccacac caacggtgcg cgatgcgttc tgtacataca ccgtcgggcc
     3901 gctgtagttc tgctcaattt tcaccgccgt gtgcgctttt gaggtcgtcg cgccgccaat
     3961 caatagcggg atggtgaagc cctgacgctc catctctttc gccacgttaa ccatctcgtc
     4021 cagcgacggc gtgataagcc ccgaaagtcc aatcagatca gcattcactt ctttagcggt
     4081 acggagaatt ttttccgcag gcaccataac gccgagatcg acaatttcgt agttgttaca
     4141 ttgcagcacc acaccaacga tatttttacc gatgtcgtgg acgtcgccct tcacggtggc
     4201 gatcaccatc ttgccgttgg ttttgccctg ctctttgctg gcttcaataa acggttcgag
     4261 atacgccacc gcctgtttca tgacgcgcgc cgatttgacc acctgtggca ggaacatttt
     4321 cccttcgcca aacagatcgc cgaccacgtt catgccgtcc atcaacgggc cttcaatcac
     4381 ttcaatcggg cgcgtagcct gctggcgggc ttcttcggta tcctgctcga taaactcggt
     4441 aatgcctttg accagcgagt attccagacg tttattcact tcccacgagc gccactccgc
     4501 ctgctgggcg ttagcggtgt cgtcggtttt gctgccgcga tatttctcgg caagctccag
     4561 taaacgctca gtgccatcat cgcgacgatt aagaatcaca tcttccaccg cgtcgcgcag
     4621 ttcagcgggc aggtcgtcgt aaatcgccag ctgcccggca ttgacgatcc ccatatccat
     4681 gccattgcga atagcgtagt agaggaacac tgcgtgaatg gcttcgcgca ccggatcgtt
     4741 gccacggaac gagaaagaaa cgttagatac gccgccggaa attagcgcgt gcggcagttc
     4801 gcgtttgatg tcttcacacg cgccgataaa gtcctgcgcg tagttgttgt gctcttcaat
     4861 gccggtggca accgcgaaga tgttcgggtc gaagatgata tcttctggcg ggaagccaac
     4921 ctcttcggtg aggattttgt acgcccgacg gcaaatctcg attttccgtg cgcgggtatc
     4981 ggcctgtccc tgttcgtcaa aggccattac caccactgcc gcaccgtaac gacgcaagag
     5041 cttcgcgtga tggataaagg cgtcgacgcc ctctttcatc gagatagagt taacaatgcc
     5101 tttgccctgg atacacttca gacctttttc aatgacatcc cattttgagg agtcgatcat
     5161 aatcggcacg cgagcgatat ccggttcacc ggcaatcaga ttgagaaaac gcaccatcgc
     5221 cgcttcggcg tcgagcatcc cttcatccat gttgatatcg ataatctgcg caccgttttc
     5281 cacctgctgg cgggccacat ccagtgcctc gctgtatttc tcttctttga tcaggcgctt
     5341 gaacttagcg gaaccggtga cgttggtgcg ttcacccacg ttcacaaaca ggctatcttc
     5401 gccaatgttc agcggctcca ggccggacaa acggcaggct acggggattt ccggcagttt
     5461 gcgcggcgct aatccttcta ctgcacgact catcgctgca atatgttgtg gcgtggtgcc
     5521 acagcagccg ccgacgatat tgagaaaacc cgcttgcgcc cattcacgta tctgttttgc
     5581 catcgtgtcg gcgtcgagat cgtactcacc aaaggcgttg ggtagcccgg cgttcgggtg
     5641 cgcggtgacg tagcattccg caatccgtga cagctcctgc acgtactggc gcagttcatc
     5701 gggccccagc gcacagttca ggccaaaggt cagagcttcg gcgtggcgca atgagttgta
     5761 aaatgcttcg gtggtctgcc cggagagcgt gcgcccggag gcgtcggtga tggtgccgga
     5821 gatcataatc ggcagctcaa cgcccagcgc ttcaaactcc gttttcaccg caaaaaccgc
     5881 cgctttggcg ttaagggtgt cgaaaacggt ttcaatcagg atcagatccg caccaccttc
     5941 caccagcgct ttggtggatt cgcgataggc cgccaccagc tggtcaaaag tgatgttgcg
     6001 gaatgccgga tcgttgacgt ccggagaaat agacgccgtg cggttggttg ggccgagaac
     6061 accggcaacg tagcgcggtt tctccggcgt gcgcgcggtc cactcgtcag cacaggcgcg
     6121 cgccagtttc gccgccgcaa agttgatttc cgccgacagg gattccatct ggtaatccgc
     6181 catcgcaatg gtcgtggagt tgaaggtgtt ggtttcgatg atatccgcgc ccgcttcaaa
     6241 gtaggcgttg tggatagcgg cgatcacttc cggtttgctg agcaccagca ggtcgttgtt
     6301 gcctttgaga tcgcatggcc agtcggcaaa gcgttcacca cgaaaatcgg cttcgttcag
     6361 tcgatagctc tggatcatgg tgcccatacc gccgtccagc accagaatac gttcatttaa
     6421 ctgcgcacgc agttgttcca ctttgctgct cacacttgct cccgacacgc tcaacccaga
     6481 tttaataaaa attcaacaaa ccatactggc ataaacgcat ctgtggtaaa agcgaccacc
     6541 acgcaacatg agatttgttc aacattaact catcggatca gttcagcaac tattgcatta
     6601 gctaacaata aaaatgaaaa tgatttccac gatacagaaa aaggaggctg tcatggtcgc
     6661 acccattccc gcgaaacgcg gcagaaaacc cgccgttgcc accgcaccag cgactggaca
     6721 ggttcagtct ttaacgcgtg gcctgaaatt actggagtgg attgccgaat ccaatggcag
     6781 tgtggcactc acagagctgg cgcaacaagc cgggttaccc aattccacga cccaccgcct
     6841 gctaaccacg atgcaacagc agggtttcgt gcgtcaggtc ggcgaactgg gacattgggc
     6901 aatcggcgca catgccttta tggtcggcag cagctttctc cagagccgta atttgttagc
     6961 gattgttcac cctatcctgc gcaatttaat ggaagagtct ggcgaaacgg tcaatatggc
     7021 ggtgcttgat caaagcgctc acgaagcgat tattatcgac caggtacagt gtacgcatct
     7081 gatgcgaatg tccgcgccta tcggcggtaa attgccgatg cacgcttccg gtgcgggtaa
     7141 agccttttta gcccaactga gcgaagaaca ggtgacgaag ctgctgcacc gcaaagggtt
     7201 acatgcctat acccacgcaa cgctggtgtc tcctgtgcat ttaaaagaag atctcgccca
     7261 aacgcgcaaa cggggttatt catttgacga tgaggaacat gcactggggc tacgttgcct
     7321 tgcagcgtgt attttcgatg agcaccgcga accgtttgcc gcaatttcaa tttccggacc
     7381 gatttcacgt attaccgatg accgcgtgac cgagtttggc gcgatggtga ttaaagcggc
     7441 gaaggaagtg acgctggcgt acggtggaat gcgctgactt tttctggcgg gcagaggcaa
     7501 tattctgccc atcatacctg agtagcaata gaataagggt gtctgttaat cgcattgacg
     7561 ccaaaataac ttaatgtcat acacttcaca cacttttatt aactcagcat tatttttaaa
     7621 catcaaacta cttaatcatt aacaaaatcg caagatctca ttccaccgaa acttttacgg
     7681 ctgcattatg agattgagcg atttatgata tttctttagt tgtaatcata ttaaattttt
     7741 tcactgttct gatagttaaa attcaagaca tcaataaaca atgagatatt taaatgatta
     7801 ctcgtatccc tcgtagttct ttctctgcaa atattaataa tacagcccag acaaatgaac
     7861 accaaaccct gagtgaattg ttttataaag aactcgagga taaattttct ggcaaggagc
     7921 tggcgacgcc tctattaaaa agcttctcag agaactgtag acaaaatggt cgtcatatct
     7981 ttagcaacaa ggattttgtc attaaatttt ccacgtccgt cttacaagct gataagaaag
     8041 aaattacgat aattaataaa aacgaaaaca cgacacttac tcaaaccatt gccccaatat
     8101 ttgaagaata cctaatggaa attttacctc aacgctcaga cactcttgat aaacaagaat
     8161 taaacctaaa atcagataga aaagaaaaag aactcccaag aattaaactt aatggtcaat
     8221 gttattttcc ggggcgaccc caaaaccgta tagtatgccg acacattgct gcacaatata
     8281 ttaatgatat ttatcagaat gttgattaca aaccccatca agatgattac tcttcagctg
     8341 aaaaatttct cactcacttc aacaaaaaat gcaaaaacca gactttggcg ttgatttcca
     8401 gccgtcctga ggggcgttgt gttgctgcct gtggtgattt cgggctagtt atgaaggcat
     8461 atttcgacaa gatggaatca aatggcatca gtgttatggc agccatatta ctggtggata
     8521 accatgcttt gacggtccgg ctaagaataa agaacacaac tgaaggatgt acccattacg
     8581 tggtttcggt ttatgatcct aatgtcacta acgataaaat aagaattatg agcgaaagca
     8641 aagaggatat taaacactat tctctgatgg attttatgaa tgtagattat agcctcctga
     8701 aatggtcaaa tgatcatatt attaaccaat ctgttgcaat aattccagca cttccgaaag
     8761 aacagctatt gatgttaaaa ggatctgtgg atgaaataac ccctccatta tcaccagcaa
     8821 cgatgaattt gctaatggca attggtcaga atcaccaact tacgcaactg atgattcagc
     8881 tccagaaaat gccagaacta catagaacag aaatgttgac tgcctataat agtggacata
     8941 tgaacgttat taatactatt tttaacgcat tacccactct gtttaatacg tttaaattcg
     9001 ataaaaaaaa tatgaagccc ctcctcctgg caaataattc taatgaatat cccggtttgt
     9061 tttcagcgat acagcataaa caacaaaatg ttgtagagac ggtttatctt gctttatcta
     9121 accatgcacg cctgtttgga tttaccgctg aagatattat ggatttttgg caacacaaag
     9181 ccccacaaaa atactctgcc tttgagttgg cttttgaatt gggtcaccgg gttattgctg
     9241 aattaatcct taatacatta aataagatgg ctgaaagctt tggctttacg gataaccctc
     9301 gatacattgc ggagaaaaat tatatggaag ctttactcaa aaaagcatct ccccataccg
     9361 tacgctaaat ctttgcctgc gccgatacgc ataaacatct tccacatgcc cttcacgtat
     9421 gcggttttgt agtgcgcgcc agtaatcagc gcggaacagg tcggcgtgca tctcttcaaa
     9481 cagcggacca atacgcgggt cggcgcatag ccagtggcga aactcttccg ggaaaacatc
     9541 gcccggcgag acgctgtacc acggttcgct ggcaagttcg tcttccggat agcgcggcgg
     9601 cgggatgtcg cggaaattca cttccgtcat gtagcaaatt tcatcgtaat cataaaaaac
     9661 cacacgccca tgacgggtga caccgaagtt tttaaacagc atgtcgccag gaaaaatgtt
     9721 agcagcggca agctggcgaa tagcgttacc gtattcttca atggcgtcgc gcaactgctg
     9781 accttccact tgttccagcc agatattgag cggcaccatc cgccgctcga tatagagatg
     9841 gcgaattaca atctgctcac caagatcggt gattttttcc gctgcttcct ggagcagtaa
     9901 ttccattaat gccggggaaa tatgccgctt ctccagcaca aagttttcaa actcctgggt
     9961 gtccgccatt cggcccacac ggtcgtgctc tttcaccagt tgatagcagg cacgaacgtg
    10021 agcggcagac atctctttct gcggcgcgaa cttgtctttg atgactttga atacccgatc
    10081 aaagcccggc agcgtaaata ccaacatcac cattccacga atacccggcg cttcaatgaa
    10141 ctgctcatta cagccctgta gataaacgag atattcgcgg tagctttcgg ttttggcgtg
    10201 cttctggcag ccgatagcca tatacaattc agcagtggtt ttacccggca gaatttcccg
    10261 tagccactcg accagcgctg cgggcagcgg tgcataaacc ataaaataag aacgcgcaaa
    10321 gccaaaaaca atgctcgctt cggcggtcgt cgtcaggcag gtatcaataa ataactcgcc
    10381 gtcgtccgtc tggtggatcg gcagtaagaa gggcaacgtg ccggaaggcg tgatcagttt
    10441 gcccaccagc caggcggctt tattgcggta aaacagttcg ttcgccacct gtaaatgact
    10501 ttccgcgagg ttgtctgtcc ccagcgtttc cgtcagatgg cgaatgatgt aatgaatgtc
    10561 acggctttta ttctgccagc gcaggcgcag cggcaggtcg ctgataacgc gcatcagtag
    10621 agattcccag ccgtgatcgg ggtgaaagtc tttcgccagc gggcggggaa tggtacgaaa
    10681 gcggcgctct ggctgagagc taaagataaa aagccgctcg ggagtaagcg agcggtggtc
    10741 aaataaccga cagtacacgg agttaaaaaa gctctccgca atctcgaagc gcgggtaatc
    10801 cggcaacagc cgcgtgtaat gctctttaac gcgcagtaaa aatgccgcgt ccgtactttg
    10861 gccgttagta atgcagcgca gttgctccac gaccagacca acgtgatgat cgtaaagatg
    10921 gatacggttt ttcatcgcct gctggacagc atgccagtcg gcctgttcga aacgctgctg
    10981 cgccccggaa gtcacttcga ggaatcgacc atactgagcg tcgaagcctt gcaaaatggt
    11041 ttgagcaatc agtaattcca ggccacgcgg catttagcgc cctcatcagg agcagagaat
    11101 tgccggatgc ggcgtaaacg ccttatccag cctacgttcg gcaacggttg taggcatgat
    11161 aagacgcgca agcgtcgcat caggcatcgt gcaccgattg ccggatgcgg cgtaaatgcc
    11221 ttatccggcc tacgttcggc aacggctgta ggcatgataa gacgcgcaag cgtcgcatca
    11281 ggcatcgtgc accgattgcc ggatgcggcg tgaacgcctt atccggccta cagtcagcaa
    11341 cggttgttgt tgcttagaac tgcgattctt cagtggagcc ggtcagcgcg gtgactgaag
    11401 acgtgccgcc ctgaataata gtcgtcactt tatcgaagta acctgtaccc acttcctgct
    11461 ggtgagatac gaaggtatag ccatctttcg cggcggcaaa ttccggctgc tgcactttct
    11521 caacgtagtg cttcataccc tcgccctggg cataggcgtt tgccaggtca aacatgttga
    11581 accacatgct gtggatacct gccagggtga tgaactggaa cttgtagccc atatccgaca
    11641 gctgctgctg gaagctggca atagttttgt cgtcgaggtt tttctgccag ttgaacgacg
    11701 gcgagcagtt ataagccagc agtttgcccg gatatttcgc gtggatagct tgtgcaaagc
    11761 gacgcgccag ttccagatcc ggcgtggagg tttcacacca gaccaggtca gcatatggcg
    11821 catacgccag gccacggctg atcgcttgct caatgcccgc atgagtacgg aagaagcctt
    11881 cactggtacg ctcgccagta ataaattcac tgtcatacgg gtcgcaatcg gaggtaatca
    11941 gatccgccgc atcggcatcg gtacgagcaa ccagcagggt tggaacgccc gtcacgtcag
    12001 ctgcaagacg cgccgcgacc agtttctgaa tagcttcctg agttggcacc aaaactttgc
    12061 cgcccatgtg accgcatttc ttcactgacg ccagctgatc ttcgaagtga actgccgctg
    12121 caccggcttc aatcatcgct ttcatcagtt caaaggcatt caggacaccg ccaaaaccgg
    12181 cttccgcatc ggcaacgatc ggcaggaagt aatcgacata gcgcggatcg cccggctcaa
    12241 tgcctgcgga ccattggatc tgatcggcac gacggaaggt gttgttgatc cgctccacca
    12301 cagctggcac cgagtttgcc ggatagagcg actgatccgg atacatgctg gccgccaggt
    12361 tagcgtccgc cgctacctgc catcccgaca gatagactgc ttcaataccc gctttcgcct
    12421 gttgcagcgc ctgaccgcca gtcagtgcac cgaggctgtt gatgtagcct tttttcgact
    12481 cgccatgcag cagacgccac attttcgccg cgcccagttg cgccagggtg cattcaggat
    12541 tgactgaacc gcgtaatttc accacatctt ccgcgctgta tgggcgagta atgccttccc
    12601 aacgcggttg agtccactct ttctgtaatt cttcaatttg ttgtgtacgg gttttcatgt
    12661 gcagatgctc catagttatg tggtggttta cgctaacagg cggtagcctg gcagggtcag
    12721 gaaatcaatt aactcatcgg aagtggtgat ctgttccatc aagcgtgcgg catcgtcaaa
    12781 acgcccctgg gagaaacgtt cttcgcccag ttcgctggca atgactttca tctcttcacc
    12841 cagcatctgg cggaacaagg ctttggtcac cggtttgcca ttgctcaacg ttttttgatg
    12901 atggatccac tgccagattg aggtacggga aatttcagcc gtcgccgcat cttccatcag
    12961 gccataaatc ggcacgcagc cgttgccgga gatccatgct tcgatgtact gcactgccac
    13021 gcgaatgttg gcgcgcatac cttcttcggt gcgttcacca tcacaaggtg ccagcagctg
    13081 atcggcagta atcggcgcgt cttgttcgcg catcacttca agctgatttt tacgggagcc
    13141 gagaatgtcg ttgaataccg ccattgccgt atccgcaagg cctgggtgag caatccatgt
    13201 gccatcgtga ccgttattgg cttccagcgc tttatccgct tttactttgt tgagcacctg
    13261 gttattgcgc tcttcatctt tgctcggaat aaacgctgcc atgccgccca tcgcaaaagc
    13321 accgcgttta tggcaggttt taatcaacag gcgtgagtaa gcattcagga atggtttatc
    13381 catcgtcact gcctgtctgt ctggcaggac gcgatcggga tagtttttca acgttttgat
    13441 atagctgaag atgtaatccc aacgaccgca gttcagacca acaatatggt cacgcagcgc
    13501 gtgaaggatt tcatccatct ggaacacggc gggcagcgtt tcaatcagca acgtcgcctt
    13561 gatggtgccg cgcggcagat taaagcgatc ttctgcatag ctgaagactt cgctccacca
    13621 ggccgcttcc tgccaggact gggttttcgg cagatagaaa tagggaccgc tgccctttgc
    13681 caacagtgcc tgatagttgt ggaagaaata gagcgcaaaa tcaaacaggc taccggggat
    13741 tgcctcacca cgccaggtga catgtttttc cggcaagtgc agaccgcgta cccgacaaat
    13801 caaaaccgct ggattgggct tgagctgata aattttgcct gcttcattgg tatagctgat
    13861 ggtgccgtta accgcatcgc gcaggttaat ttgcccgtcg atcactttgt tccagtctgg
    13921 tgccagtgaa tcttcgaaat cggccataaa gactttcaca ttggcgttca gtgcgttgat
    13981 caccatcttg cgctcaaccg ggccagttat ctcgactcga cgatcttgta agtccgcggg
    14041 aataccacga attttccagt ccgtatcgcg aatggaagct atttccgaaa taaaatcagg
    14101 caaagttccg ttatcaatat cttgctgctg ctgaatgcgc gctgccagaa gtttattgcg
    14161 ttgtggcgta aaatgcgtca ccagctcagt cagaaattct accgcttcgg cagtaagaat
    14221 ttgcttctcc tgctcgccat acggccttat gaaagccagt tcatcggttg ttgttgcctg
    14281 ttcagtcatc gtgcagctcc tcgtcatgga tccgaaaact tccccactgt gaacgaagga
    14341 tcgttgtgcg cttttcgttc agcacaacta agactactca tttaaaatgc aaaatcaaaa
    14401 acaatttcca ttttaattaa aaatacttga taacttattg ataacaatat gaataaaatt
    14461 tattcacctc ctgaggtgcg tttcggaaat aaaaaaggca cccgaaggtg cctgaggtaa
    14521 ggtgctgaat cgcttaacga tcgactatca cagaagatta atccagcgtt ggattcatgt
    14581 gccgtagatc gtatggcgtg atctgataga cgtaataatt gagccagttg gtaaacagca
    14641 aattaccgtg actacgccag ctcgctcgcg gtgtattttg cggatcattg tgcgggaaat
    14701 agttatacgg tacttccggg cctagtccgg cttccacatc gcggaaatat tcctgcgcca
    14761 gcgtttgcgc atcatattcg ggatggcccg tcacaaaggc aatgcgctta tctttgctgg
    14821 caaacagata tgcatcccct tcttccgtct ctgccagaat ttccagatcg gtgtaatcac
    14881 gaatcaacgc tgccggaaag tcagcatagc gcgaatgtgg tgccaggaat gaatcatcaa
    14941 agccacgcgt cagaagcgca tgaggatgga gaatatgatg ctcgtaaacg ccagagagtt
    15001 tgtcggtgcg agtttgctta ggaatgccgt agaggatatt gagcgcggcc tgtaccgccc
    15061 agcagacaaa cagcgtcgag gtgacgtgat ctttcgacca ctccagcacc tgtttgatct
    15121 gcggccagta agcgacatca ttaaactcca ccaggcccag cggcgcaccg gttacaatca
    15181 gaccgtcaaa gttctgttcc tgaatatctt caaagttaca gtagaagttg ttcagatgct
    15241 ctgcgggcgt gttgcgcgat tcacgggaat cgatgcgcaa cagctgaata tcgacctgca
    15301 aaggtgagtt tgaaagcagg cgcagaaact gattttcagt ttcaatcttc ttcggcatca
    15361 ggttaaggat cagaacctta agtggacgaa tttcctgacc agacgcacga gaagttgtca
    15421 tcacaaagac gttttcttca cgcaagaaat tgacggcggg tagctcgtcc ggcacacgaa
    15481 taggcataac ctgattacct cactacatac gcttatacgt ttagacatcc agatagctga
    15541 agataaccag aaaatttgct aatgtcgagc ctgcatgttg aaggtgagaa agtttcaagg
    15601 aaaaggctac gttagaatat aagaatgacg aaaaggagag aaaatggtta ttagtattcg
    15661 ccgctcacga catgaggaag gggagggact cgttgcgatt tggtgtcgat ctgtcgatgc
    15721 cactcacgat tttttatcag cagagtatcg ggccgagctg gaggagctgg ttcgttcctt
    15781 tctgccggaa gcaccgttgt gggtcgcggt taatgagcgg gaacagccgg ttgggtttat
    15841 gttgttaagt gggcagcata tggatgcgct gtttatcgat cctgatgtgc gcggctgcgg
    15901 cgtgggtcgg atgctggtgg agcatgcgct ctcgatggca ccggaactga caaccaacgt
    15961 taatgagcaa aatgagcagg cggttgggtt ctataagaag gtgggtttta aggttacggg
    16021 acgctctgag gtggacgatt tggggaaacc gtatccattg ctgaatctgg catatgtcgg
    16081 ggcgtaggcg tttttttctc atcccgctgg gctgaacttc aggtttcgta cccaccaatg
    16141 gtgacacgat tatttctgct taagcattgc catgaccgca ctatcactga gtaccactga
    16201 ttgcgcatgg atgtggaact ggcgatattt cattaacgtt gccccagcga ccacttcatg
    16261 aagaatacgt tcctcaacct gtgacaaacc gccctcattc atttccagcg cgctaacaac
    16321 aatatccata cgttttgctg gcaaaaaaga atgccaggtt gaacgtgggt ggagttgcag
    16381 aacaagatca tgcagaactt tttctgcaac aaagaaattg gcgattaaca ggcgctggtt
    16441 actgaaggca gcgtctcctg acacttcacg tttgctttca agatcgcgaa cagtaatttt
    16501 attacgacta atttggatat acagaactga catgagattc ccttcatcat gcaaataatt
    16561 gatatgcaaa aaaccccggc cttatggacc ggggtttttc taatttgatg cctggcagtt
    16621 ccctactctc gcatggggag accccacact accatcggcg ctacggcgtt tcacttctga
    16681 gttcggcatg gggtcaggtg ggaccaccgc gctacggccg ccaggcaaat tctgttttat
    16741 taacccgctt atcgccggtt aatctaatct gtatcaggct gaaaatcttc tctcatc
//
LOCUS       DAMZET010000090        16230 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.090, whole genome shotgun sequence.
ACCESSION   DAMZET010000090 DAMZET010000000
VERSION     DAMZET010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.090"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            111..2111
                     /locus_tag="POB34_002971"
     CDS             111..2111
                     /locus_tag="POB34_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HDI5919875.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCTTAQPTEKEE
                     AWDPAIWLEELATLPDATRKRAQALVGKGITIELFCTPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFANDEG
                     NACRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHVRASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            2236..2697
                     /locus_tag="POB34_002972"
     CDS             2236..2697
                     /locus_tag="POB34_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HDI5919876.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(2738..3208)
                     /locus_tag="POB34_002973"
     CDS             complement(2738..3208)
                     /locus_tag="POB34_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HDI5919877.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(3255..3974)
                     /gene="yehT"
                     /locus_tag="POB34_002974"
     CDS             complement(3255..3974)
                     /gene="yehT"
                     /locus_tag="POB34_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HDI5919878.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(3971..5656)
                     /gene="btsS"
                     /locus_tag="POB34_002975"
     CDS             complement(3971..5656)
                     /gene="btsS"
                     /locus_tag="POB34_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HDI5919879.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            5878..6609
                     /gene="mlrA"
                     /locus_tag="POB34_002976"
     CDS             5878..6609
                     /gene="mlrA"
                     /locus_tag="POB34_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HDI5919880.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            6669..6776
                     /locus_tag="POB34_002977"
     CDS             6669..6776
                     /locus_tag="POB34_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HDI5919881.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(6757..7488)
                     /gene="yehW"
                     /locus_tag="POB34_002978"
     CDS             complement(6757..7488)
                     /gene="yehW"
                     /locus_tag="POB34_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HDI5919882.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPALQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(7493..8419)
                     /gene="yehX"
                     /locus_tag="POB34_002979"
     CDS             complement(7493..8419)
                     /gene="yehX"
                     /locus_tag="POB34_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HDI5919883.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(8412..9569)
                     /gene="yehY"
                     /locus_tag="POB34_002980"
     CDS             complement(8412..9569)
                     /gene="yehY"
                     /locus_tag="POB34_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HDI5919884.1"
                     /translation="MTYLRINPVLALLLLLTVIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSIFALILVQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(9576..10493)
                     /gene="osmF"
                     /locus_tag="POB34_002981"
     CDS             complement(9576..10493)
                     /gene="osmF"
                     /locus_tag="POB34_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HDI5919885.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(10704..13001)
                     /gene="bglX"
                     /locus_tag="POB34_002982"
     CDS             complement(10704..13001)
                     /gene="bglX"
                     /locus_tag="POB34_002982"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HDI5919886.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            13197..14912
                     /gene="dld"
                     /locus_tag="POB34_002983"
     CDS             13197..14912
                     /gene="dld"
                     /locus_tag="POB34_002983"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDI5919887.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(14950..15882)
                     /gene="pbpG"
                     /locus_tag="POB34_002984"
     CDS             complement(14950..15882)
                     /gene="pbpG"
                     /locus_tag="POB34_002984"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HDI5919888.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(16056..>16230)
                     /locus_tag="POB34_002985"
     CDS             complement(16056..>16230)
                     /locus_tag="POB34_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919889.1"
                     /translation="ALFYTGYLLYLGIPSFLNINKEEGLSFSSSTLAIGVLVLEVLLA
                     LTVILWGYGYRLF"
BASE COUNT         3950 a         4437 c         4084 g         3759 t
ORIGIN      
        1 acaccttgct atgaccacga tacggcccag gcgctggtaa atgtcggcgc acaaatagcc
       61 gccatgacac cgggcgagct ggcatcatgg cttgcggaga atcttcagtc atgaattcac
      121 tacgtccgga attattagaa ctgacaccgc aggccctgac ggcgttaagc aatgccggtt
      181 ttgttaagcg cagtcttaag gaactggaaa atggcaacgt cccggaaatc agccatgaga
      241 acagcgcttt aatcgccacc ttcagtgacg gtgtccgtac ccagctggcg aacggccagg
      301 cactgaaaga ggctcagtgc agctgcgggg ccagcggtat gtgccgtcat cgcgtgatgc
      361 ttgtgttaag ttatcaacga ctttgtacta ccgcccagcc tacggaaaaa gaagaagcgt
      421 gggatccggc aatctggctg gaagaactgg ctaccctgcc cgatgccacc cgcaaacgcg
      481 cacaggcgtt ggtcggtaaa ggcatcacca ttgagttgtt ctgtacgccg ggcgaaattc
      541 cctctgcccg cttaccgatg agcgatgtgc gtttttattc ccgcagcagt attcgtttcg
      601 cccgttgtga ttgtattgaa ggcacacttt gcgaacatgt cgtactggct gtgcaggcct
      661 tcgtcgaggc caaaactcag caagcggaat ttactcattt aatctggcag atgcgcagcg
      721 aacacgtcac atcatctgac gatccgtttg ccaacgacga gggtaacgcg tgtcgtcaat
      781 atgttcagca attaagccag gcattatggc tgggcggcat cagccagccg cttattcact
      841 acgaggccgc tttcagtcgc gcgcagcagg cggcggaacg ctgcaactgg cgatgggtga
      901 gtgaatcact acgacagcta cgggcaagcg ttgatgcctt ccacgtccgc gccagccact
      961 atcatgccgg agaatgctta cgtcagcttg cggcattaaa cagtcgatta aattgcgcac
     1021 aagagatggc ccggcgcgac agtgttggtg aagttcctcc tgtgccgtgg cgcacggtcg
     1081 ttggctctgg cattgccgga gaagcaaagc ttgatcatct gcggctggtg tctttaggta
     1141 tgcgttgctg gcaggatatt gagcattatg gtttacgcat ctggtttacc gatcccgaca
     1201 ccggcagtat tttgcacctt tcgcgcagtt ggccgcgaag tgaacaggaa aactcaccgg
     1261 cagctacgcg tcggctgttt agttttcagg ctggcgcact ggcgggtggg caaattgttt
     1321 cacaagcagc aaaacgcagt gccgatggcg agctgctgtt agctacccgc aaccgcttaa
     1381 gcagcgttgt gccgctgtcg cctgatgcct ggcaaatgtt gagcgcgccg ttacgccagc
     1441 cgggcattgt ggctttgcgg gaatatttac gccagcgtcc ccccgcctgc atacggcctc
     1501 ttaatcaggt cgataactta tttattctgc cggtcgctga gtgtatttcg ctcggttggg
     1561 atagcagccg ccagacgctg gatgcgcagg taatcagcgg cgaaggtgaa gataatctcc
     1621 tgacgttatc ccttccggca tctgcctgtt ctccttttgc cgttgaacgc atggcggcgc
     1681 ttttgcaaca aacagacgac cccgtgagtc tggtttctgg ctttgtcagt tttgttgatg
     1741 ggcaattgac actggaacca cgggtgatga tgacaaaaac ccgcgcctgg gcgctggacg
     1801 cagaaactgc gcctgtggca ccgctacctt ctgccagcgt tttgcctgtg ccgtctaccg
     1861 ctcatcagtt gctgatgcgc tgccaggcgt tacttattca actgctccat aacggctggc
     1921 gctatcagga acagagcgct attagtcagg cagagttgct ggcgaatgac ctctccgcgg
     1981 tcggttttta tcggctggca catgtgttgg gacaatttcg taatacagaa agcgaggcac
     2041 gggtagaagc aatgaataac ggtgttttac tttgcgaaca attattcccc atgcttcagc
     2101 aacaaggatg aaatagtgct ttttactaag agttctactc cagttccgga ctgctcacgt
     2161 cacggtatta ggcatattct atatagcccc tggtgagagt caccagtttc ttgattaaat
     2221 aaaatggagt tttacatgaa ggctttcaat aagctgtttt ccctcgttgt tgcatctgtt
     2281 ctggttttct ctcttgctgg ctgcggtgac aaagaagaat cgaagaaatt cagcgccaat
     2341 ctgaacggca ctgaaattgc cattacctat gtctacaaag gtgacaaggt gcttaagcaa
     2401 tcttctgaaa ccaaaattca atttgcttct attggtgcaa ccaccaaaga agacgctgcc
     2461 aagacacttg agccgttaag cgccaaatac aaaaacatcg cgggtgttga agaaaaatta
     2521 acctatacag atacctacgc gcaggaaaac gtgactatcg atatggaaaa agtggatttt
     2581 aaagccctgc agggtatttc aggaatcaac gtttctgctg aagatgccaa aaaaggtatc
     2641 actatggcgc aaatggaact ggtgatgaaa gccgctggtt ttaaagaagt gaaataatct
     2701 gtcggcggcc atgtttcgca tggccgccat acccgtctta gcttttcttc acatgctggc
     2761 gcgctgccag tccacgcaga aaataacgta aaaattgatc gccgcattcg cggaagtttt
     2821 tatgatccgg tgcgcgcatc atcgctgtaa tttccggcat cgaaacgcgg aactgctgtt
     2881 cggtgaggat agccagaatg tcatcggttt tcagcgaaaa cgcgatgcgt aattttttca
     2941 gcacgatgtt gttattaatg cgacgttccg gctccagtgc cggagcagac tcatccttgc
     3001 cgcgtttttc ataaatcagg ccattgagga atgacgacaa aacaatgtcc ggacaacgct
     3061 gaaaaccctc ttcgtcttct ttacgtagcc agacggcgat ctgttccgcg gtggcttcga
     3121 cattacccag cgccagaata cgcaccaggt cattattatt ggctttcaaa atgtagcgca
     3181 cgctgcgcag aatatcgtta cttagcatga ggccttcagg tgttgatgag gcaaaaagcc
     3241 attttagcag tcttttacag gccaatcgcc tcttttaagc ttttcagata acggcggctg
     3301 accggcacgg ttaagccatt acgcaaaatc aactcggcct ggccgttatc ttccagacga
     3361 atctcctgta aatgcgcgag gttaaccaga tactgacgat ggcagcgcag tagtggtgta
     3421 cgactttcca gggtacgtaa tgtcaattcg gtaaagccct ctttcccttc gtggctggta
     3481 acgtagacac cgctcatccg actgctgaca aatgccacat ctttcatttg cagcaaataa
     3541 atccgactat gccccgtaca agggataaat ttcagcgcct gttgattttc cggtaacagc
     3601 gaaacatcct gcttgctgcg ttcctgacgc aaacgcgcca gcgttttctc cagtcgcgct
     3661 tcatcaattg gcttcagcag ataatcaaag gcatgttctt caaaggcttt aatagcgtat
     3721 tcgtcaaacg cggtgagaaa aacaatatac ggacgatgtt ccgggtcgag catccccacc
     3781 atttccagac cactgatgcg cggcatctgg atatcgagaa acagcacatc cgggcgcagt
     3841 ttatgcaccg cgccgatccc ttccacggcg tttgaacact ctccaacgat ttcaatatcg
     3901 ctctgctcct gcaaaaatac gcgcaggttc tcccgtgcta acggttcatc atcgacaatt
     3961 aagactttaa tcatgcctcg tccctccatg gtagtcgtaa cgttattcgg gtgtaactat
     4021 caggctcaca ggcgacgctt attccatagt catcgccaaa ccgttcacgt aaacgcttat
     4081 ccaccagatt catccccagc ccactggcat tggttaccgg ttgatacaaa ccggcattgt
     4141 cttcgatctc cagcatcaaa tgttgcccct cacgtcgggc gctgattgcc actcgccctg
     4201 tatccagcag ttgtgatgtc ccatgtttaa tggcgttttc cactatcggt tgcagggtaa
     4261 acgcgggcaa ttgctgctgg gataattctt gcggaatagc aatgttgacc tgcaaccgcg
     4321 actggaagcg cgccttttca atttgcagat aagcgttcac atgttcaatt tcgtcggcga
     4381 gagtaacaaa ctccgaaggc cgctttaagt ttttgcggaa aaaagtggaa agatactgca
     4441 ccagctggct ggcctgttcg ctgtcgcggc ggatcaccgc tttaatggtg ttaagcgcat
     4501 taaacaaaaa atgaggattc acctgggcgt gaagcagttt gatttctgac tgggtgagca
     4561 tcgctttttg ccgctcatat tgcccggcaa gaatctgcgc cgaaagcagt tgcgcaatcc
     4621 cctcgcccag cgtgcggttt attgaactga ataaacggtt tttggcttca tacaatttga
     4681 tggtgcccat cacgcgctga ttttcgccac gcaacggaat taccagcgtc gaccccagtt
     4741 tgcattgcgg atgcaaagag caacgataag gtacttcgtt gccatcagcg tagaccactt
     4801 caccggtttc aatcgctttt aaggtgtaag tcgaagaaat cggtttgccg ggtaaatggt
     4861 ggtcgtcacc aattccggta aaggccagca atttctctcg atcggtaatc gcgactgcac
     4921 caatatccag ctcctgatac agcacctgag ccactttcat gctgttcact tcgttaaacc
     4981 cctgtcgcaa aatgccttcc gtcgaggctg ccactttcag cgcagtggca gaaaaagcag
     5041 aagtgtattt ttcaaacatc gcgcgtttat cgagcaatat acgcataaac agcgccgcgc
     5101 cgacggtatt ggtgaccatc attggtgcag caatattact caccagacgc accgcatctt
     5161 cataaggtcg ggcgatcgca aggatgatca gcatttgcac catttcagcg acgaacgtga
     5221 cggcaccggc ggtaatgggg ttaaagactt tatcagtgcg cccgcgacgg atcaggatgc
     5281 tgtgtaccag gccgccgagc aatccttcaa cgatggttga gatcatgcaa cttagcgcgg
     5341 tcatgccccc catcgaatat cgatgtaagc cgccggtcag accaaccagc ccaccgacga
     5401 ccggaccgcc gagtaagccg cccattaccg cgcctatcgc acgggtattg gcaatagaat
     5461 cgtcaatgtg caacccaaac caggtgccca tgatgcagaa gatggaaaag acgatgtagc
     5521 agagaaattt atgcggcaga cgaaccgtga cctgcattaa cggtatgaat aatggcgttt
     5581 tactcattaa ccacgcaatg actaaaaaaa cgcacatctg ctgaagcagc agcaacacca
     5641 gattaaaatc gtacataccc gcaaaccaca cttcccttta aaacgcgtaa catacattgc
     5701 ctgcgtttaa ctttctttga actcttgcag aaaaatgaga attcgtgagt acgatcactc
     5761 aaaatcgcct ggcaaaaata aaatcaccct atagatgcac aaaaaacggg caaaactacc
     5821 tggttcgcaa aactgcgtct aaagttaaac cgggacctcg cgagcaaggg tgagacgatg
     5881 gcgctttaca caattggtga agtggcgttg ctttgtgata ttaaccctgt cacgttacgc
     5941 gcgtggcaga ggcgttacgg gttgctgaaa ccgcaacgga cagacggcgg tcatcgactg
     6001 ttcaacgatg ccgatattga ccgtatccgc gagatcaaac gctggatcga caacggcgtg
     6061 caggtcagca aagttaaaat gctgctcagt aatgaaaatg ttgatgtgca gaacggctgg
     6121 cgcgatcagc aagaaacatt actgacctac ctgcaaagcg gcaatctgca tagcctgcga
     6181 acgtggatca aagagcgcgg tcaggattac cccgcccaga cactcaccac acatctgttt
     6241 attcctctgc gccgacgact tcaatgccaa caaccgactc tccaggcgct gctggcgatc
     6301 ctcgacggcg tactgatcaa ctacatcgcc atttgtctgg cttcggcacg taaaaaacag
     6361 ggtaaagatg cgctggtggt tggctggaat attcaggata ccacccgtct gtggctggag
     6421 ggctggattg ccagtcaaca aggatggcgc attgatgtcc tcgcccactc gctcaatcaa
     6481 ctacgccctg aactattcga aggccgtaca ttgctggtgt ggtgcggtga aaatcgaacc
     6541 tccgcccaac agcagcaact caccagttgg caagaacaag gccatgatat tttcccactc
     6601 ggcatttaat gattcgttaa caaatgcgct ttactgtaca atcctttcgt taacataagg
     6661 agtgcaatat gcgcatagct aaaattgggg tcatcgccct gttcctgttt atggcgttag
     6721 gcggaattgg tggcgtcatg ctcgcaggtt atacctttat tttgcgtgct ggctaagcgc
     6781 ctgcaccagc ctttcaaaca ggcggtctgc gatgatcgcc gccagtgcca ccagtaacgc
     6841 cccctggatc acatacgcgg tattaaatcc gctaagcccg atgatgatgg gcgtacccag
     6901 cgtgctggcc cctaccgttg aggcgatcgt cgccgtacca atgttgataa tcaccgaagt
     6961 tcgcacgccc gccagaatca ccggagccgc cagcggtagc tcgaccttac gcagtcgctg
     7021 accacgactc attcccatac ctttcgcaac ttctgtcacg ctggcatcaa tcgctcccag
     7081 cccggcaagt gtcgcctgca gggcgggcag cacaccgtaa aggatcaagg cgataatcgc
     7141 tggttgcaga ccaaagccga tcaccggaac ggcaatcgcc agcactgcga caggcggaaa
     7201 agtctgtcca acggcggcaa tagtttccac cagtgggcga aattccgcgc cccacgggcg
     7261 agtgacagca attccggcac cagtgccaat gatcaccgca aacaaactcg aaattcccac
     7321 cagccagaaa tgagccagtg ccagagctgc aaaactttct tgctgataaa cgggtcgtgg
     7381 cagttgtggg aacaaggcag caaacagcgg ctggctgtaa ggcagccaga aaatcagtgc
     7441 cacaaacaaa gcaatgagcc agaacagcgg atcgcgcaac atcttcatac gcttacgcct
     7501 cctccagcag atcctgaaaa tgcagcgtgc cgcaaggctg gccctgcgtg ttcaccaccg
     7561 gcagcacctc gcatccccgc gcgacaaaca gggagagcgc atcgcgtagc gtcatctctt
     7621 ctgccagtgc ctcaccttct gcccgttctt cgcgacgcac gtaatccgcc acactacgta
     7681 acgaaagcag gcgcacaccc agttcactac gtccaaaaaa ctggcggaca aaatcattcg
     7741 ccggacgagt cagcatcgtc agcggattcc cctgctgcac cacttcaccg tgatccatca
     7801 ataccagatg ttctgccagc cttagcgcct catcaatatc atgagtgacc agcacaatag
     7861 tgcgccccag caaacggtga atgcgcgtca tctcttgttg caacgcgccg cgcgttaccg
     7921 ggtccagtgc gccaaaaggt tcatccatca gtaagacttg cggatcggca gccagtgcgc
     7981 gcgccactcc cacacgttgc tgctgaccac cggaaagctg atgcggataa cgctcacgca
     8041 aatttgactc cagccccagt agcgccatta attcgtcgat acgatcgtca atccgcgccc
     8101 gcgaccattt ttgcaattgc ggcacggtag cgatgttttg cgccacgctc cagtggggga
     8161 acaggccaat agattgaatg gcatagccca tccggcggcg caactccagt actggcagcg
     8221 agcgaatttc ttctccggca aagcggatca cgccgctgtc atgttccacc aggcggttaa
     8281 tcattttcag ggtggtggat ttgccggagc cagatgtgcc aatcagcacc gaaaaactcc
     8341 cttcctgaaa attgagattg agatcgttaa cggctttttg tgcgccaaac agtttgctga
     8401 catggctaaa ttcaatcatt acgtttcacc ttcagcagtg cgataagcaa atcgaacagc
     8461 gcgtcggtaa gcaccgccag aacaattacc gggatcaccc ccagcaacac taaatcaatg
     8521 gcgctgctta gcagcccctg gaaaaccagc gcaccaaaac cgcctgcgcc gattaacgcc
     8581 gcaatcaccg ccatacctac agtttgcacc atcaccaccc gcaggctgcg cagaaatacc
     8641 ggtaacgcca gcggtaactg aacatgcagg aaacgctgcg ccccgctcat ccccatcgct
     8701 ctggcgctct ccagcacatc gcgcgggatc tggttcaaac cgactaccac gccgcgcacc
     8761 agcggcagca aggcatagag caccagcgca atcagtgcgg gtgtcattcc ggttcctgct
     8821 atgccgagct tccccagcca cggaaaggcc gtaaccagcg cggcaagcgg cgcaatcaac
     8881 aggccaaaga gcgccaccga aggcacggtc tgaatgacat tgagcagaga aaaaattgcc
     8941 ccctgccgcg cagtggaaaa gtagcaccag atgcccaacg gcacaccaat cactaacgca
     9001 ggcagcaccg caccaaacag caacgtcaga tgttgtgcca gcgcgtcgtc aaacacatcc
     9061 tgacggttgg cgtattcttt cattagtgag agatcgttaa gcgtgccgga gtacagcaac
     9121 cacagcggaa taatggcaat ctgcatatgc aacaaccagc gccacagcgg atgcgtggag
     9181 attcggcgga tggcatcgct acaggccagc aatgccagcg ccgcagccag ccagaaacca
     9241 ctgccgaggc tggtacgcgc cagcgcactg ccattttgcg ccagttgggt cgccgccttt
     9301 ccagctcccc acaccagcaa tacgaagacg aattgcacca gaatgagtgc aaaaatgctg
     9361 ccttttttac cgggaataaa acaggctgtc agccaggcgc aaccaacgcc caccagcatc
     9421 cagatcgttt gcggccacag ctgccagaga tgtcgccctt cacccgagac taaacgatta
     9481 ggcgcgtaac tgataaacgg cagcgccgct gcgattaccg tcagcaacag cagcagcgcc
     9541 agaacaggat taatacgtag ataagtcacg ggaaattact tcgcccaccc tttttgtttc
     9601 aggtagtcgg cagccacttt tttggcatcc agtccttcca ctgcaatgct ggcattcagt
     9661 tgctgcaatg tttttgcatc gaggctggcg aagactggct gtagccactg tgccatttgc
     9721 ggatactcct tcaacaccga ttcacgcacc actggtgcag gcgcatagat aggttgcacg
     9781 ccttgcggat cgcttaaggt ttgcagcccc agcgccgcta ccgggccgtc agtgccgtaa
     9841 gccattgcag cattaacgcc agaggtttgc tgggcagcgg ctttgatcgt caccgccgtg
     9901 tcgccaccag ccagtgacag caactgatcc tgaccgagct taaagccata ggctttttca
     9961 aacgcgggta acgcatcggc gcgttcgata aactctgccg aggctgccag tttgaaggtg
    10021 ccgccctctt gcagataacg actcaggtcg gcaagcgaag tgagtttatt tttctctgcc
    10081 acatcctgac gcacggcgat agtccaggtg ttatttgcag gcgcgggcgt cagccagatt
    10141 aacttgttgt gctccgaatc gagctttttg actttctcgt aaccttgctg cgcgtttttc
    10201 cacgctgcat cgttttcatc tttaaagaag aaagcgccat tgccggtata ttccggatag
    10261 atatccagtt caccggaagt aatcgccccg cgcaccacag gagtcgttcc gagttgcact
    10321 ttattcaccg tcggtacgcc gtggctttcc agcacctgca aaatgatatt gccgagtagc
    10381 gcaccttcgg tatcgatttt tgaaccgact ttaacggggg aagccgcctg tagcggcagg
    10441 ctcacggctg ccaacataac cagtgaacct gcccagagct ttaagagtgg catgatgctt
    10501 tcctcattct tttactgttg ttttcagcga attaagagaa aagcatagtt gataatggcg
    10561 gggttagctt gagcggaagg attcagttgc agaatcagat aaatacttaa ggggcatatt
    10621 cggtccggcg tttcctgccg gatgcggcgc gagcgcctta tccggcctac aaagggcgca
    10681 aacgtcgcgc ccttactaaa gcattacagc aactcaaact cgcctttctt aacgcgtgcg
    10741 gaatcagtgc cgataaatac attgaacttg ccaggctcgg cgtcatattt catctgttga
    10801 ttccagaact tcagcgcctc aatatcgatc gggaagctga cagtctgagt ttcgcccggt
    10861 ttcagggtga ttttctcaaa gcctttcagc tgtttcacag ggcgactcat ggaagccgtc
    10921 acatcctgca agtacatctg cactaccgtg gccccctcgc gcttaccggt gttcgtcacc
    10981 tgcacgctgg cagtcacttt gccgtcacgc ttcatggtcg gcgcagaaag tttcacatca
    11041 gagacggtga aagtggtgta gctcagccca tagccgaacg gatacaacgc cccgttagct
    11101 tcatcaaaat aacgcgaagt gtatttgttc ggcttgtcgg cattatacgg gcgaccggta
    11161 ttcagatggc tgtagtacac cgggatctgc ccgacagaac gcgggaagga cattggcagc
    11221 ttgccggacg ggttgtaatc gccaaacaat acatcggcaa ttgcattacc gccttcagtc
    11281 cccgcaaacc aggtttccag aatcgcatca gcctgctgat cttctttcac cagcgccagc
    11341 ggacgcccgt tcatcagcac cagcaccagc ggtttaccgg tggctttcag cgccgcaatc
    11401 aagtcacgtt ggctttgcgg aatagtgata tcggtccggc tggaggcttc gtgcgccatc
    11461 ccctgtgctt caccgactac agccaccacc acatcagatt gtttcgccgt ctgcaccgct
    11521 tcatcaatca tctcttgcgg cgaacgcgga tcgactttga ccgcttcttc atactgattc
    11581 aggaaatcga taatgccttt gtcactggta acgttcgccc ctttggcata cagcacttta
    11641 ccgttttcac cgacggaatt tttaatcccg gtcagtacgg tcacggattg atcggcaaca
    11701 ccggctgcgg accagctgcc catcacgtca cgtttactgt ccgccagtgg cccaaccacc
    11761 gcaatggtgg ccgatttttt cagcggtaac gtttcgagac ggtttttcag caacaccagg
    11821 ctttcgcgcg ccacttcacg cgcttcttta cggtgcaggc ggctttcggc attggtatcc
    11881 accgggtcag actctttcgg ccccaaatgg ctgtatgggt cgttaaacaa tcccatatca
    11941 tatttaacgt tcagtacatg gcgggcagca tcgtccagct cttccatcgt cactttgccg
    12001 gatttgatca acccaggcag atacttcgag taatactcgt cgctcatact catgttgatt
    12061 ccggatttca gcgccacgcg caccgcatct tccgggtctg ccgccgtgcc atgtttaatc
    12121 agctctttga ttgcaccgtg atcggaaacg gtgatgcctt taaagcccca ctggtcgcgc
    12181 agaacatctt tcagcagcca ggaatcggag gtggctggcg tgccgttcag cgagttcagc
    12241 gccaccatca ccgcgccgct gcctgcgtcc agccccgctt tgtacggcgg catataatca
    12301 ttaaacaggc gctgcggact catatcgacg gtgttgtact ctttaccgcc ttctaccgcg
    12361 ccgtatgcgg caaagtgttt gacgctggtc atcaccgagt agcgatctgc cgggctttta
    12421 ccctgcatcg cttccaccat ggttttaccc attgttgagg tgagatacgt atcttcgcca
    12481 aaaccttcgg aagcacgtcc ccagcgcgga tcgcgcgaga catcgaccat cggtgcccag
    12541 gtcatattca ggccatcatc tgccgcttca taagcagaga cacgtccgac cgttttcact
    12601 gcatcgaggt taaaagacga ggccagaccg aggctaatcg ggaacaccgt gcgctgaccg
    12661 tgcagcacgt cgtaagcaaa gaaaagagga attttcaggc ggcttaattc catcacctga
    12721 tcctgcatgg cgcggatatc ctgacgggtt acggtgttga aaatcgcccc aacctgaccg
    12781 tctttgatca tctcgcggat cgcctctttc gggttatccg ggccgacgct gattaagcgc
    12841 agctgaccaa ttttctcatc aactgtcatt ttcttaagca gttcggtgac gaacgcatcc
    12901 cgcgcttcgg gcgttaatgg atggttgccg aataaatcat ccgccagtgc tggctgcagg
    12961 gccagactca ccgcgattcc tactgaacat agccatttca tatggatttt tcttcctctg
    13021 ttgcccgacg aggcagcgaa aatggtgcaa aaaccgtagt ttgccataag catgatggag
    13081 agagaaaaag aatgctcagt ttattgtctg aattttcaaa atattcactc gctgaattgt
    13141 catacaaggc gctattctag tttgtgatat tttttcgcca ccacaaggag tggaaaatgt
    13201 cttccatgac aacaactgat aataaagcct ttttgaatga acttgcccgt ctggtcggtc
    13261 attcacacct gctcaccgat cccgcaaaaa cggcccgcta tcgcaagggc ttccgttctg
    13321 gtcagggcga cgcgctggct gtcgttttcc ctggctcact actggaattg tggcgggtgc
    13381 tgaaagcctg tgtcaccgcc gacaaaatta ttctgatgca ggctgccaat acaggcctga
    13441 ccgaaggatc gacgccaaac ggtaacgact atgatcgcga tatcgtgatc atcagcaccc
    13501 tgcgtctcga caagctgcac gttcttggca agggcgaaca ggtgctggcc tatccgggca
    13561 ccacgctcta ttcgctggaa aaagccctca aaccgctggg acgcgaaccg cactcggtga
    13621 ttggatcgtc gtgtataggc gcatcggtta tcggcggtat ttgtaacaac tccggcggct
    13681 cgctggtgca acgtggcccg gcgtataccg aaatgtcgtt attcgcgcgt ataaatgaag
    13741 acggcaaact gacgctggtg aaccatctgg ggattgatct gggcgaaacg ccggagcaga
    13801 tccttagcaa gctggatgat gatcgcatca aagatgacga tgtgcgtcac gatggtcgtc
    13861 acgcccacga ttatgactat gtccaccgcg ttcgtgatat tgaagccgac acgcccgcac
    13921 gttataacgc cgatccggat cggttatttg aatcttctgg ttgcgccggg aagctggccg
    13981 tctttgcggt acgtctagat accttcgaag cggaaaaaaa tcagcaggtg ttttatatcg
    14041 gcaccaacca gccggaagtg ctgaccgaaa tccgccgtca tattctggct aacttcgaaa
    14101 atctgccggt tgccggggaa tatatgcacc gggatatcta cgatattgcg gaaaaatacg
    14161 gcaaagacac cttcctgatg attgataagt taggcaccga caagatgccg ttcttcttta
    14221 atctcaaggg acgcaccgat gcgatgctgg agaaagtgaa attcttccgt ccgcatttta
    14281 ctgaccgtgc gatgcaaaaa ttcggtcacc tgttccccag ccatttaccg ccgcgcatga
    14341 aaaactggcg cgataaatac gagcatcatc tgctgttaaa aatggcgggc gatggcgtgg
    14401 gcgaagccaa atcgtggttg gtggattatt tcaaacaggc cgaaggcgat ttctttgtct
    14461 gtacgccgga ggaaggcagc aaagcgtttt tacaccgttt cgccgctgcg ggcgcagcaa
    14521 ttcgttatca ggcggtgcat tccgatgaag tcgaagacat tctggcgttg gatatcgctc
    14581 tgcggcgtaa cgacaccgag tggtatgagc atttaccgcc ggagatcgac agccagctgg
    14641 tgcacaagct ctattacggc cattttatgt gctatgtctt ccatcaggat tacatagtga
    14701 aaaaaggcgt ggatgtgcat gcgttaaaag aacagatgct ggaactgcta cagcagcgcg
    14761 gcgcgcaata ccctgccgag cataacgtcg gtcatttgta taaagcaccg gagacgttgc
    14821 agaagttcta tcgcgagaac gatccgacca acagcatgaa tccggggatc ggtaaaacca
    14881 gtaaacggaa aaactggcag gaagtggagt aaaaattacg gatggcagag tatcgccatc
    14941 cgaattcact taatcgttct gtgccgtctg ccccgccgcc gccatttggg cggctttttg
    15001 ttttttatag ctcaacgctg ctgccggcac aggcatcact ttaccggttt caatccaggt
    15061 acgcaggcgg ctggcatcgg caaaatgggt atatttgcca aacgcgtcca tcactaccag
    15121 cgccaccggt ttattattga taaccgtacg catcaccaga caatggcccg ccgcattggt
    15181 aaagccggtt ttggttaact gaatattcca gttatcgcga tacaccagat gattagtatt
    15241 gcggaacggc agcgtatacg tcgggttaga gaaggttgcc atatcttccc gggtagtact
    15301 taactgcccg atcaacggat attgtttgct ggcaatgagc agtttggtta agtcacgggc
    15361 agttgaaacg ttatgcaccg acaatccggt aggttcaaca aagcgcgtgt tgttcattcc
    15421 gagcgatttc gctttcgcat tcattgcctt aataaaggct ttgtaaccac cgggataatg
    15481 gtgtgcaagg cttgccgccg cgcggttttc tgaagacatc agcgccagca acagcatatc
    15541 tttacggctg atttcgctat tcagtcgtac gcgcgaatag acccctttca tctccggcgt
    15601 ctggctgata tccactttta gtttttcatc cagcggcagt cgtgcatcca gcacaaccat
    15661 cgcggtcatt aatttgctga tagacgcaat cggacgcacc agatccgggt ggttcgaata
    15721 gatcactttg ttggtattca gatcaacaat catcgcgcta ccggaggcaa tttccggttg
    15781 tgaagcggtg gtagcggctg ccgttttcgc aacggcctgc ggtgcaaaag gcacagccag
    15841 catcagggcc aggctaaata aagaaactcg aaatttcggc atgatgagca ttcagatagt
    15901 ggttcacgcg cacgggttgc gcaccgccgg agtaaggatt tactgaggct agcgacgcca
    15961 tcataacgag caaaaagtgc gatcgtcaaa ggagaatcgt gaggaaatgc tgcattgctg
    16021 acatttacgc cagcaatgca acgtcagaga actttctaga acaaacgata accgtagccc
    16081 catagtataa cggttagggc gagcagcact tccagtacca gcacgccaat cgccagcgtc
    16141 gaactagaga agctaaggcc ttcctctttg ttgatattca ggaagctcgg aataccaagg
    16201 taaagcaggt agccggtgta aaacagcgcc
//
LOCUS       DAMZET010000091        16016 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.091, whole genome shotgun sequence.
ACCESSION   DAMZET010000091 DAMZET010000000
VERSION     DAMZET010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.091"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..266)
                     /locus_tag="POB34_002986"
     CDS             complement(<1..266)
                     /locus_tag="POB34_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HDI5919890.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYG"
     gene            complement(335..1801)
                     /gene="guaB"
                     /locus_tag="POB34_002987"
     CDS             complement(335..1801)
                     /gene="guaB"
                     /locus_tag="POB34_002987"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDI5919891.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            1963..3333
                     /gene="xseA"
                     /locus_tag="POB34_002988"
     CDS             1963..3333
                     /gene="xseA"
                     /locus_tag="POB34_002988"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDI5919892.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(3330..3545)
                     /locus_tag="POB34_002989"
     CDS             complement(3330..3545)
                     /locus_tag="POB34_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDI5919893.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(3614..5086)
                     /gene="der"
                     /locus_tag="POB34_002990"
     CDS             complement(3614..5086)
                     /gene="der"
                     /locus_tag="POB34_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDI5919894.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(5204..6382)
                     /gene="bamB"
                     /locus_tag="POB34_002991"
     CDS             complement(5204..6382)
                     /gene="bamB"
                     /locus_tag="POB34_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HDI5919895.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(6393..7013)
                     /gene="yfgM"
                     /locus_tag="POB34_002992"
     CDS             complement(6393..7013)
                     /gene="yfgM"
                     /locus_tag="POB34_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HDI5919896.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(7031..8305)
                     /gene="hisS"
                     /locus_tag="POB34_002993"
     CDS             complement(7031..8305)
                     /gene="hisS"
                     /locus_tag="POB34_002993"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDI5919897.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(8416..9534)
                     /gene="ispG"
                     /locus_tag="POB34_002994"
                     /gene_synonym="gcpE"
     CDS             complement(8416..9534)
                     /gene="ispG"
                     /locus_tag="POB34_002994"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDI5919898.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(9561..10574)
                     /gene="rodZ"
                     /locus_tag="POB34_002995"
     CDS             complement(9561..10574)
                     /gene="rodZ"
                     /locus_tag="POB34_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HDI5919899.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(10859..12013)
                     /gene="trmG/rlmN"
                     /locus_tag="POB34_002996"
     CDS             complement(10859..12013)
                     /gene="trmG/rlmN"
                     /locus_tag="POB34_002996"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HDI5919900.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(12163..12594)
                     /gene="ndk"
                     /locus_tag="POB34_002997"
     CDS             complement(12163..12594)
                     /gene="ndk"
                     /locus_tag="POB34_002997"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDI5919901.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(12743..15055)
                     /gene="pbpC"
                     /locus_tag="POB34_002998"
     CDS             complement(12743..15055)
                     /gene="pbpC"
                     /locus_tag="POB34_002998"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HDI5919902.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDHWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAKLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(15056..>16016)
                     /locus_tag="POB34_002999"
     CDS             complement(15056..>16016)
                     /locus_tag="POB34_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HDI5919903.1"
                     /translation="ADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
BASE COUNT         3776 a         4538 c         3978 g         3724 t
ORIGIN      
        1 atgccatagc aaacgccgaa taccggtacg cctgcttcaa agacatactg cggcgcacgc
       61 gggctgtttt cttcggtggt gctttccggg ccgccggaaa gaataatgcc gcttggattg
      121 aagtcacgaa tttgtgcttc tgtcacatcc cacgcccaca gttcgcagta aacacccagc
      181 tcacgcacgc ggcgcgcaac cagttgagtg tactgagaac cgaagtccag aatgaggatg
      241 cgatgcttat gaatgttttc cgtcattgac gcttattccg aggcaagtga aacagataat
      301 ataaatcgcc cgacatgaag tcgggcgaag agaatcagga gcccagacgg tagttcgggg
      361 actctttagt aatggtcacg tcgtgaacgt ggctttcctg aatgcccgca ccgctgatac
      421 gtacaaactc cgctttagta cgcagttcgt cgatagtacc acagccggtc agacccatac
      481 aggagcgcag gccgcccatc tgctggtgaa tgatctcttt caggcgacct ttataagcta
      541 cgcgaccttc gataccttcc ggcaccagtt tgtcggcagc gttatcgctc tggaaataac
      601 ggtcagagga acctttggac atcgcgccca gggaacccat accacggtaa gatttgtaag
      661 aacggccctg gtagagttcg atttcacccg gagattcttc agtacccgcc agcatggaac
      721 ctaccatcac cgcgcttgcg ccagcggcga tagctttggc gatgtcgccg gagaagcgaa
      781 taccaccatc tgcgataacc ggaataccgg tgccttccag ggcttcaact gcgtcagcaa
      841 cggcagtgat ctgcggaaca cctacgccag taacgatacg agtcgtacag atagaaccag
      901 ggccgatacc gactttaacc gcgctgcaac cagcttctgc caaagcgcgt gcacctgcag
      961 ctgttgccac gttgccgccg atgatttgca gatccggata tttagcacgg gtttcacgga
     1021 tacgctgcaa aacgccttct gagtgaccgt gggaggagtc gatcagcaga acgtcaacgc
     1081 cagcggcaac cagcgcgtca acacgctctt cgttacccgc acctgcgcca actgcagcac
     1141 caacacgcag acggccttgc tcgtctttac aggcgttcgg tttacgttcc gctttctgga
     1201 agtctttcac ggtgatcatg ccgatcaggt ggaattcgtc atcaaccacc agcgcttttt
     1261 caacgcgttt ttcgtgcatt tttgccagca ccacttcacg ggcttcacct tcacgcacgg
     1321 tgaccagacg ctctttcggc gtcatgtaaa cgctaaccgg ctggttcagg tcggtaacaa
     1381 aacgcacgtc acgaccggtg ataataccca ccagttcgtt ttcttcggta acgaccggat
     1441 agcccgcaaa accgttacgc tcagtcagtt ctttcacttc gcgcagcgtc gtggttggca
     1501 gaacagtctg cggatcagtc accacaccag attcgtgttt tttcacacgg cgaacttctt
     1561 ctgcctggcg ttcaatggac atgtttttgt ggataaagcc gataccgcct tcctgagcca
     1621 gagcaatagc caggcgcgct tccgttacgg tatccattgc tgcggaaagc atagggatat
     1681 tcagacgaat agttttcgtc agctgggtgc tgaggtcagc agtattcggc agaacggtag
     1741 aatgagcagg aacgaggaga acgtcgtcaa acgtcagagc ttctttagcg atacgtagca
     1801 tgggcaatat ctcgaccaga gtggttaata aatattgccg cggcattata cagagcgtaa
     1861 ccgattgcat ctaccccttt ttgcaaaaaa tgcttgctat ccccgaaggg cgggttacta
     1921 tcgactgaat aacctgctga tttagaattt gatctcgctc acatgttacc ttctcaatcc
     1981 cctgcaattt ttaccgttag tcgcctgaat caaacggttc gtctgctgct tgagcatgag
     2041 atgggacagg tttggatcag cggcgaaatc tctaatttca cacaaccggc ttccggtcac
     2101 tggtacttta cactcaaaga cgacaccgcc caggtacgct gcgcgatgtt ccgcaacagc
     2161 aaccgccggg tgaccttccg tccacagcat ggacaacaag ttttagttcg cgccaatatt
     2221 acgctctacg agccgcgcgg tgactaccag ataatcgttg agagtatgca gccggccggt
     2281 gaagggctgc tgcaacagaa gtacgaacag ctcaaagcga agttgcaggc tgaaggtttg
     2341 ttcgatcagc aatacaaaaa accacttccc tcccctgcgc attgcgttgg tgtgatcacc
     2401 tcaaaaaccg gtgctgcgct acatgatatt ttgcatgtgt taaaacgtcg cgatccttct
     2461 ctaccggtga tcatctaccc caccgccgtt cagggcgatg acgcaccggg gcaaattgtt
     2521 cgcgccattg aactggcgaa tcagcgcaat gagtgcgacg tgttgatcgt tgggcgcggc
     2581 ggcggttcgc tggaagattt atggagtttt aacgacgaac gcgtagcgcg ggcgattttt
     2641 gccagccgca ttccggtcgt cagcgccgtc gggcatgaga cggatgtgac cattgccgat
     2701 tttgttgccg atctgcgtgc gccaacgccg tctgccgccg ctgaagtagt gagccgtaat
     2761 cagcaagagt tactgcgcca ggtgcaatcg acccgtcaac ggctggagat ggcgatggat
     2821 tattatctcg ccaaccgcac gcgtcgcttt acgcagatcc atcaccgatt acagcaacag
     2881 catccgcagc tccggctggc acgccagcaa accatgcttg agcgcctgca aaagcggatg
     2941 agctttgcgc tggaaaatca acttaagcgt accgggcaac agcagcagcg gttaacacag
     3001 cggctgaatc agcaaaatcc acagccgaag attcatcgcg cgcaaacgcg cattcagcaa
     3061 ctggaatatc gtttagcaga aaccctgcgc gtacagctta gcgccacgcg tgaacgtttc
     3121 ggtaatgcag taacgcacct cgaagccgta agcccactgt caacgctggc gcgtggatac
     3181 agcgttacta ctgctactga cggcaatgta ctgaaaaaag tgaagcaagt taaagcgggt
     3241 gaaatgctaa ccacacgtct ggaagacggc tggatagaaa gtgaagttaa aaacatccag
     3301 ccagtaaaaa aatcgcgtaa aaaggtgcat taagccagca cgaattcaac gcgttttttc
     3361 gatatcaagc catgcccgtg ctgacagaag taatctactg caccgcaggc ttttaacacc
     3421 tgcaacggtt gatggcagtc tggacaaaga gctttcattt caataatttc cccgcaggat
     3481 gggcaacgcg catggccgtt atcctgatca agcacatgct gacattgtgg acaatgcagt
     3541 tccatatgaa ttcctccggt agccatccct ctacattcat agagggaatg gcagataaaa
     3601 tacttacgga taattattta tttttcttga tgtgcttcat cagacgctta cgtttacgca
     3661 tctgggttgg cgtcagggtg ttacgcttgt tcgcatacgg gttttcccct tctttgaact
     3721 gaatacgaat cggcgatccc attacgtcca gcgatttgcg gaagtagttc atcaagtagc
     3781 gcttgtagga atcaggcagg tctttcacct gattaccgtg aatcaccaca atcggcgggt
     3841 tataaccacc agcgtgggca tatttcagct tcacacgacg accgcgtacc agcggcggtt
     3901 ggtgatcttc aacagccatc gtcatgatgc gcgtcagcat agaggtcccc acacgacggg
     3961 tggagctgtc atacgcttca cgtactgatt caaacaagtt accaacacca ctgccgtgca
     4021 aggcagagat aaagtgcaca cgagcaaaat cgataaagcc cagacggaag tccagcgttt
     4081 ctttcacctg ctctttcact tcctggctca ggccatccca cttattcacc acaatgacaa
     4141 gtgagcgccc actattgaga ataaagccca gcagcgagag atcctgatcg gaaatacctt
     4201 cgcgcgcatc aatcactaac atcaccacgt tggcatcttc aatggcctgc aacgttttga
     4261 ttacggagaa tttctctaca gcatcggtga ttttgccgcg tttacgtacg ccagcggtgt
     4321 caatgagcac atactcacgt ccatcgcgtt ccattggaat gtagatactg tcacgcgtcg
     4381 tgccaggcat gtcataaaca acaacgcgct cttcaccaag aatacggtta gtgagtgtag
     4441 acttacctac gttcggacga cccacaatcg ccagtttgat cggcagactt tgcgggtcga
     4501 agtcgtcttc ctcttcttct tcgccgttct cttccgcttc aaattgcgcc cagtattcag
     4561 cgtcttcgtc gacttcctct tgcggtgcga gatcttccat ccacggcagc agcacatgct
     4621 ccagcagact taatacgcca cgaccgtgag acgcggcgat cgggtagatt tcacctaaac
     4681 caagcgagta gaaatcaacc actgcctgat cgggatccag accgtcagtt ttgtttgcca
     4741 ccaggaaggt cggtttttca cgggagcgca gatgtttggc aatcgcttca tctgccggca
     4801 tcaggcccgc gcgcgcatcc accataaaca gtacgacgtc cgcttcttca atcgccagca
     4861 gcgactgttc cgccatgcgg gtttctacac cgtcttctgt gccatcaatc ccgccggtat
     4921 caatacagat aaactcacgg ccttcaattt ccgcacgacc gtacttacgg tcacgagtca
     4981 gacccgggaa atccgcaacc agcgcatctc gggtgcgagt tagacggtta aataacgtgg
     5041 attttcctac gttagggcgc ccgacaagcg cgaccacagg taccatgttt aaagcctcat
     5101 ttttataaat catcagacaa cgcacgctat attcgcgtcg ttgttaaaaa caggaaaacg
     5161 gcccctgtcc aggagccgtt ttcaaagtga acgacagaga cgattaacgt gtaatagagt
     5221 acacggttcc gtcttttgcc tggatcagca gtttgccgtc agcggcaacc ggttcagtct
     5281 ggaaaccgga actatcaact ttttgctggg caacgaaacg accatcttcg acgttaatcc
     5341 agtgcagata accttcactg tcaccgacca ccaggttgcc attatacagc accggagaag
     5401 tcagcaggcg atgcagcaga tcgctttgtg cccacagcgt aacgccgcca tcaatggtca
     5461 acgccatcac ccggtcattt tgatcgacca gatagatgcg attgccgtcg acgatgaaat
     5521 cattcaccga acccagttcg cgtttccaca taatctgacc actgcgcaga tcaagcgccg
     5581 tcaggttacc attataggcc agcgcgaaaa caacgccgtt aacaacgacg ggagtcgtgt
     5641 caacatcgct cagacggtca atttcggtag aaccggtcgc ctgggaaata cgctgctgcc
     5701 aaatcatctg gccctgttcc atcagcactg cgctgacgcg accattatcg ccccccacga
     5761 cggccgcacc aaaagccgtt gccggcgcag actcgccacg caaagagagc gaaggcatat
     5821 cgaggttaac tgtccatttg acagcgccgt cagcttcgtt cagcgcttgt aactgaccgt
     5881 tactggtgtg gattaacacc agaccgtcgc tgaccaccgg gcgcgaaagt gcttcacccg
     5941 cgactttagt ttgccatgcc acagtaccat cgctggtatt cagcgcgtaa acctgcgcct
     6001 tttcgctgcc aatgtagaca tgcccaccag acacggtcac accgccagaa agtaatgcag
     6061 gctctttaga gaaccagcca tctttctcgg ccaggctgac agaccagatt tctttgccat
     6121 catccgcatt cagcgctttt accaaaccag cgcggtccgc tgcatagaca acgttgtccg
     6181 ccagtgccgg atgaagattg gaatagaagt tgccaatgcc gctaccaacg gaagtactcc
     6241 acgccgtggt cggcgtaaac tggttttcaa cggttggcaa tggggacatc tttaccacat
     6301 cttcttcgct gttaaacagc gaacagccgc ttaaaagggt aacggaaagc agtcctggca
     6361 gcagtaattt acgcaattgc atcgggtccc tctcagatgg acaaattatt aattttcatc
     6421 tgcatcattt cgctcagtgc cggagtaaca tcgcttttca cgcctgcttc ccatgcacta
     6481 cgcgcacctt gcttatcacc tttgctcagc aatgcttcac cacgcaggtc ggcaacaatg
     6541 gcagcccacc cttcaccttt gatggtatca agggttttca gcgcggcatc agcctgcttg
     6601 agctgtacct gaacgcgagc aagacgcaga tttatcacgg ctttgagatt ttcatcgctc
     6661 gtgtctgcca gcccctgttg taactgggcg gcagctttct ctagttcatt tttgtcaaca
     6721 aattgctgcg caagttccaa agaagccagc gcaccataag tatttttatt ttcagcagca
     6781 aatttttccg ccgccgggat gctatccggt ttgccttcgc tcactgcggt aaccgcattt
     6841 tgataggcaa gagaagcgga gcgtgcagaa tcaacctgat ggctgttcca gtagcgccag
     6901 ccaatcagtg cgccaacgcc caaaatcacc ccaacagcca gtgctttgcc attttcagca
     6961 aaaaagcgtt taaccgcttc tacctggtcg ttttcgttct cgtaaatttc cacgctgtcc
     7021 ttctccttcc ttaacccagt aacgtgcgca aatgcgcggc tacgctatcc tgcgcaactg
     7081 ccgtttgctc accagagcgc aaatccttca ctactgctgt gccgttagcc acttcagact
     7141 cacccagcac cacagcaacg cgggcacccc atttatcagc acgggcaaac tgtttcttaa
     7201 agttgccgcc gccgtggttg gtcatcaatt tcacgcccgg taattcatca cgcagacgct
     7261 cagctaatgc catagccgca gattgtgtat cagcacctga agccaccagg tatatatcga
     7321 caacaggatc ggctttaaat tccggattaa cggcctgtac taacaataca agacgttcga
     7381 ggcccatcgc aaaaccgaca gccggtgttg cacgaccgcc cagttgttcc acaagaccgt
     7441 cataacgacc gcctgcacac acggtgccct gggagccgag actgttagtc acccactcga
     7501 aaacggtacg gttatagtaa tccagaccac gcaccagacg ctggtttacg gtgtaagcga
     7561 tccccgcgct ctcaagcagt ttgcacagac cggcaaaatg ctcacgagat tcctcgtcca
     7621 gataatcacc taatgccgga gcgtcgttga gaagcgcctg cacttccgga ttttttgaat
     7681 ccagcacgcg cagcgggtta gtgtacatgc ggcgtttgca gtcttcgtcc agcttttctt
     7741 tatgctgctc aaggaatgcc accagcgcat cgcggtaatt ggcgcgtgct tccagcgaac
     7801 cgatagaatt cagctcaaga gttacgtgtt cggaaatacc cagcgcgcgc caccagcggg
     7861 cggtgagcat aatcagttca gcgtcgatat ccggaccttg cagaccgaaa acttcgcagc
     7921 ccaactgatg gaactgacga taacgccctt tctgcggacg ctcgtgacgg aacatcggcc
     7981 cgatatacca cagacgctgt tcctgattgt acagaagacc atgctcgatg ccggcgcgta
     8041 cacagcccgc cgtcccttca gggcgcagag tcaggctgtc gccattgcga tcctcaaagg
     8101 tgtacatctc tttttcaacc acgtcggtga cttcaccaat cgcacgtttg aatagcgggg
     8161 tctgctctac aatcggcaag cggatttcac tgtaaccgta gctgccgagc acgtttttca
     8221 gtgtgccttc aatgcgctgc cagatggccg tttcgccagg caggtaatcg ttcatgccgc
     8281 gaatggcttg aatgtttttt gccacgttta ttctctttct gaatataaaa atgaaccctc
     8341 aacgcttccc tcaatgtttc gggagctatg cgggttcaat catacacggg aagcgaggcg
     8401 cttcccatca cgttattatt tttcaacctg ctgaacgtca attcgacgcg cttcgtccag
     8461 ctgactggct ttcgcacgaa tgcgtgcttc cagctggtca atcatatcgt tgttgtccag
     8521 acggtctttg cgcacgccat cttcatagag gccgcttttc ttgttgccgc cggtgacgcc
     8581 gagtgtagaa accagcgcct cacctgggcc attcaccacg cagccgataa tcgaaacgtc
     8641 catcggagtg atgatatctt ccaggcgttg ctccagcgcg ttaaccgtac cgataacatc
     8701 aaattcctga cgcgaacagg tcgggcaggc gatgaagttg atcccgcgcg aacggatacg
     8761 cagcgatttc aaaatatcga aaccgacttt gatctcttcg accggatcgg ccgccagtga
     8821 tacgcgcagc gtgtcgccga tgccttcaga cagcagcaga cctaaaccaa tggcggattt
     8881 tactgccccg ctgcgtgcac caccggcttc ggtgatcccc agatgcagcg gctgatcgat
     8941 ctgttttgcc agcaaacgat aagactcaac agcgaggaag acgtcagacg ctttcacgct
     9001 gactttgaac tgatcgaagt tcaggcgatc gagatgatca acatggcgca tggcggattc
     9061 cagcaacgcc tgcggcgtcg gttcgccata cttttcttgc agatcttttt ccagcgatcc
     9121 ggcgttaacg ccaatacgga tcggaatgtt tttatcgcgc gcacagtcaa ccaccatgcg
     9181 aatacgctct tcattaccga tattgccagg gttaatacgc agacaatcga cgccgtattc
     9241 cgctactttc agtgcaatgc gatagtcgaa gtggatgtca gccaccagcg gcacgttaac
     9301 ctgctgtttg atgagtttga acgcttctgc cgcgtccatc gtcggtacgg agacacggac
     9361 gatatcagcg ccaacgcgtt ccagcgcctt gatttgattg accgttgctt cgacgtctgt
     9421 cgtacgcgta ttggtcatgg actgtacggc gatgggagca ccatcgccaa tcggcacatt
     9481 cccaacgtaa atacgtgttg attttctacg ttgaattgga gcctggttat gcatgaaaaa
     9541 tctcccgcgt tacccgtctg ttactgcgcc ggtgattgtt cggcattgag ggtcagacgc
     9601 gcaacctggt tagttctgat aaaacgactc agatcgacag gtttcccttg atactggatc
     9661 tgtactgcgg ctggcgcacc aattttcagt ttgtatggtg cctggccggt taagttcaaa
     9721 ttaccgtctt tacgctgcat accgctaaac aattttttac cggtagcatc agtgacctcc
     9781 agccagcaat cggcagtaaa gttcatcacc agcgcattcg gatcagccac cggcgtggtc
     9841 acgccagcct gatcggttgg caacggcgca gcaccatctg gcgttgttgc tgccgttggt
     9901 gccggggtcg cggcggtatc aacatttgcc tgcgaaggcg aaacaaccgc attctgttgc
     9961 ggatcaacag ctggtgctgg cgcagttacg gcaggtgttt gcgtgttggt tgcggtagta
    10021 tccacagacg ctggcggcgt ggacgttgta gccgggtctg tagttgtcga cgtatttaac
    10081 ggaacactct gcccctgctc gctattactg ctcagttccg ccgaagattg atcggccata
    10141 gtggtgatct cttcctgctg agctttgtgg tcttgccacc accaggcacc gctcaggccg
    10201 ataaccacaa acaacaccag ccaggtgaag gtcatcagcc agccgtcacg ttttttgcgg
    10261 cgtttaccga gggaaaaact ctgcatcggc gcaactttcg cagcccgtag tggagcctgc
    10321 ttttccagcc ctggcagcag ttcttcttct ggaatatgta ccagacgcgc ataagagcgg
    10381 atatatccgc gcaggaatgt tgaagcaaga tcggcgggtg ccttatcttc ttcaatgtcg
    10441 cgcaccgtgg aaaccttcag gcaaagtcgc tcggcaacgg cctgctgact aagtcctagt
    10501 tgttcgcgag cattacgcag gcgagcgccg gtagtaagtg cttcattttg gtcgtgcgtg
    10561 gcttcagtat tcattcgctg caggtacgtt taaatgagaa ttgagatgcc ggtgaaccat
    10621 catgctcacc cacaccgcga aacatccgtt aagttaaccg ttatcataca gtataagacc
    10681 gtctgtgtgc tcgtgacaaa gcccacattc actaaggcta acttactgtt gcatcgttac
    10741 atactgcctt aaagtcagca aaaacgcacc gttaatattg accagacaat tgcaacttaa
    10801 tacttcattc ataaaagatt acgccacggt acataaagta accgtggcgt aatggctatc
    10861 agaccgcttt aatgtcgatg gcttcaccct gcatccgttt acgcagggta cgtttcgtac
    10921 ggtcgataac atcgcccgcc aactgaccac aggcagcatc gatatcatca ccacgggttt
    10981 tacgcacaat agtggtgaaa ccgtagctca tcagcacttt tgagaaacgg tcgatacggc
    11041 tgttcgagct gcgtccatac ggcgcgcccg ggaaggggtt ccacgggatc aggttgatct
    11101 tacacggcgt atctttcagc agttccgcca gttggtgcgc gtgttcagtg ccgtcgttaa
    11161 cgtggtcaag catcacatac tcaatagtga cacggccctg attggcgttg gatttctcca
    11221 gataacggcg caccgcagca aggaacgttt cgatattgta ctttttgttg atcggcacaa
    11281 tttcgtcacg aatttcgtcg ttcggcgcgt gcagggaaat tgccagtgca acgtcgatca
    11341 tatcgcccag tttatccagc gccggaacta caccggaagt agaaagcgtg acgcgacgtt
    11401 tagacaggcc aaaaccgaaa tcatcaagca tgatttccat cgccggaacg acgttgttca
    11461 ggttgagcag cggctcgccc atgcccatca tcactacgtt agtgattggg cgctgaccgg
    11521 tgacttttgc tgcgccgacg attttcgccg cacgccacac ctggccaata atttccgata
    11581 cccgcaggtt gcggttaaag ccctgctggg cggtggaaca gaatttacac tccagcgcac
    11641 accccacctg cgaagagacg cagagcgtgg cacggtcgtc ttccgggata tacaccgttt
    11701 cgacgcgctg atcgccaacg gcgatcgccc atttaatggt gccgtcggat gaacgctgtt
    11761 cttcaaccac ttccggtgcg cggatctccg ccacctcttt cagtttgccg cgcaacactt
    11821 tgttgatgtc ggtcatctca tcaaagttgt cgcagcaata gtgatacatc cacttcatca
    11881 cctgatcggc gcggaagggt ttttcaccta aatctttaaa aaactcccgc atctgctgac
    11941 ggttgagatc cagcaggttg atttttccat ctttcgtggt gacgttttca ggtgtgacta
    12001 attgttcaga catatgctat tccggcctcg ttattacacg ttatggcccc tggagggttg
    12061 aaaaaagaaa cgccccggtg agcggctgct cgtccggggg cgctgcattg tacaaattct
    12121 ggcgcacgga tgccacgttt gcacgcggca tttacgaaat tattaacggg tgcgcgggca
    12181 cacttcgcct tcgccaaaga aataagcgat ttcgcgagcg gcagattcga cggaatcaga
    12241 accgtgggta ccgttttcgg tcaggctgtc agcgtaatca gcgcgcagag taccagccag
    12301 tgcgtttgcc ggattggtcg cgcccagcag atcgcggtga cgctgaacgg cgttttcacc
    12361 ttccagcacg gaaaccacga tcgggccaga ggtcatgaat tcaaccagac catcaaagaa
    12421 cggttttcca tcgtgttcag cataaaagcc acgtgcctgt tcaacggtca ggtgcagcat
    12481 tttggtgccg acaattttga accctgcagc ttcaaagcgc gcaaagatat taccaatgac
    12541 gttttttgct accgcgttcg gtttgatgat ggaaaaagta cgttcaatag ccatttttac
    12601 ctctgtaaat tgttctgttg ttgtctgtac cagcgtacga gatggcgcgg attataatga
    12661 gcaacagggc cgttgactat tgatgaaggt aacatttttt taaaataaaa tgagttttag
    12721 caacaatcag gggcaaaaca gactattgca tgacaaattt cactgtcgcg atttgcccca
    12781 catcatccat caccagcaat tgataatcac ctttatccgt caaatgcagg gtcacgttgc
    12841 gcccgcgttc agttaacggt tcgccattca gaaaccacca gcgttcacct gcccctccac
    12901 ttgattgcaa cggcaaagtc gcttccgctg cgcccggtaa acgtttaata atcgcgccat
    12961 cgcgaacgcc tgtcagctgc agagggagct tggcatcgtg accgtatggc gggcaaattg
    13021 tcgaggccgg tggtaagcgc acagcgcgac gttctgatgc gggcagccag ggttccagcg
    13081 gtagcggcca gacatttatc atttcttgtc gcgcttgcgg gcaatcagcg gcaacacgtt
    13141 tgccattttc atccagccag atggggaaac gaatgccatt aatgccttcc tgctccggca
    13201 gtaatagagt tggcggctga ctcccgtcca gcagccaggt tgccaggcgg cggcgacagt
    13261 taccgtcacc ttccggcaaa gactgtccgc ccggccagca gataacgcca cgagtgactg
    13321 agttcgggcg cgggtcttcc ggcagattcg cactgcgcga cagtaagata ttattgacct
    13381 gattcaacaa tggtacggca ctggcaaagc caaactgacc aacaacgggc gtgccgtccg
    13441 gtctgccagt ccagatccca atgacatagc gagcgttaac cccaatcgcc caggcgtcac
    13501 gatagccata gctggtgccc gttttccatg ccagtggggc gacgcgcggc aaggcactat
    13561 ccggcaaggg tttcgcttca tcagccataa tccggcgaat gatccacgcc gcccccgacg
    13621 acattaaagg ccgttcaagc agcggatcgt caggctgtaa gcgcaatttg cctgccttgc
    13681 cgtggcgagc aaacgcggta tacgctgccg ccatatcttc cagttttgca ccagcgccgc
    13741 cgagaatgag tgaaagattc ggcgcagcac cgttgggcaa atataacggc aatccaacat
    13801 tgcgtaactt tgccgcaaac cgtttcggtc cataggcttc cagcacctgc acagcaggta
    13861 agttcagcga gcgcaccagc gcctcgctca tgctgatcgg gccatgaaaa ccgctatcaa
    13921 agttacctgg tcgataatca ccggtgcgcc gggggacgtc ttgcagcagt gatgccgggt
    13981 ggatcaagcc ttcatccagc gccagaccat aaacaaacgg tttgagcacc gatcctggcg
    14041 atcggatcgc attgaccata tcaacatgac caaagcgtga atcatcgttg agatcaaccg
    14101 atcccaccca gccgcgaaca cgcatatcgg tatgatcaac cacgatcatc gccagtgagc
    14161 tgcgcggtgg caaccgccct ttccagtttt gcgccagttc ttccagacgt cgttgaagac
    14221 cggcatccag cgtagtaacg attttgtcgc ttttgctttt accgagcatc atgcgcgaaa
    14281 acagcggtgc cagttgcggc atttgtcggg gtgccagcca gatgggttct tcccttgact
    14341 cttttacctg ctcacgggac cacacacctt gcacggccat ccgttcgagc actttattac
    14401 gcgcggcttc ggcacgctcc ggccaacgat ccgggcgaag acggctgggc gcttgcggca
    14461 aaaccgccag cattgccgcc tcggaatagc ttaaattcgc aggcgacttt ccgagataag
    14521 cccaacttgc cgcaccgatc ccctgcaacg taccgccaaa cggagcgcgg ttaagataca
    14581 aggtcagaat ttcacgctta gacagatgcc attccagttg caacgcgcgc cagagctggc
    14641 gaattttgcc gccaaatgtt ttggggtgag gatcaagcag acgagcaacc tgcatagtga
    14701 gcgtgctgcc accggaaata acccgtcccg aagtgagatc ttgccatgct gcgcgcgcca
    14761 ccgagaatgg attcaccccc ggatgcttcc agaaccagtg atcttcataa ttgatcagcg
    14821 cttcaaggta acgtggagaa acatcttcga ttgttaccgg ataacgccag atgccgtcag
    14881 catcggcgaa gcgccatagc ggcgtaccat cctgcgccac gaccactcgt gcgggattga
    14941 cttcatgcag cggtagaggc cagagtttat ctgctcccca cgctgcaaga aaaaggagaa
    15001 atggcgcagc tgccagcgtt atccagcagc cgcgtttggt taacaagcga ggcatttacg
    15061 gtcggacaat cagcagatct tcagccgcgc cggtcgcccg ccattgggga acatacattg
    15121 attccaccat cggttgcggt acctgatacg ttcccggcgt caccgcccgc gccagataca
    15181 ccagcgttac cggttggtat tcatcaacgg caaccgccgc cacaaagcga tcgtcacgga
    15241 actcaatgtg cttaatactc gcctgctgca tctggttcag taagttctgc acttcgccac
    15301 cactttgctc caggctggcg ctaccgttcg ccagattctg attttccagt tccagacccg
    15361 caggcagcag atccacgact aacgcatccg gcacgctgtt actggctttt acctgcaacc
    15421 agaccagcac cagatcgccg ctacgtaacg agtccagcga tttgctctta ccatcagtac
    15481 caagaatatg acgctcgatt tgcagcacat tgttcgccgg taaaggtgcg gattgcggat
    15541 aaccgctggc atccatacgc aaccataacg gctgatcgcc actgtttctc acctgcaagg
    15601 tggcaagttg atcgctattc agattgctgt ttagcgtttt ctcgcctgtc agcggctcag
    15661 ctgagaaaga ggtttgcgcc tgccatttac ccggtaaatc ctgaatcgta cgggcagcta
    15721 ggaacaacgc gttactttcc tgcgtcgata gccagcgttc accaaacgcc tgctgcgaaa
    15781 gtgtgttcag caaagtgtac tgctcatcgg gtagcagttt attttcttcc agcaaggaga
    15841 gcattaacgc gttgtcgcgc agtggactac cgtaatcacc cagccatatc cgctcatcac
    15901 tatttctcgg cgttttcagc gccagcacaa tcgcttcttc accacgcgtt gcatcaccca
    15961 tggttttcag cgcaacgcca agttgcagca gcggtaaacc agaagcggca tctgca
//
LOCUS       DAMZET010000092        16004 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.092, whole genome shotgun sequence.
ACCESSION   DAMZET010000092 DAMZET010000000
VERSION     DAMZET010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.092"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..634)
                     /locus_tag="POB34_003000"
     CDS             complement(<1..634)
                     /locus_tag="POB34_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDI5919904.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKY"
     gene            830..1738
                     /locus_tag="POB34_003001"
     CDS             830..1738
                     /locus_tag="POB34_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDI5919905.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            1735..2996
                     /locus_tag="POB34_003002"
                     /pseudo
     CDS             1735..2996
                     /locus_tag="POB34_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
     gene            2993..3631
                     /locus_tag="POB34_003003"
     CDS             2993..3631
                     /locus_tag="POB34_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDI5919906.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            3636..4412
                     /locus_tag="POB34_003004"
     CDS             3636..4412
                     /locus_tag="POB34_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDI5919907.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            4501..5865
                     /locus_tag="POB34_003005"
     CDS             4501..5865
                     /locus_tag="POB34_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HDI5919908.1"
                     /translation="MSTITLLCIALTGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(5959..6951)
                     /gene="rpoS"
                     /locus_tag="POB34_003006"
     CDS             complement(5959..6951)
                     /gene="rpoS"
                     /locus_tag="POB34_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HDI5919909.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(7014..8153)
                     /gene="nlpD"
                     /locus_tag="POB34_003007"
     CDS             complement(7014..8153)
                     /gene="nlpD"
                     /locus_tag="POB34_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HDI5919910.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8293..8919)
                     /gene="pcm"
                     /locus_tag="POB34_003008"
     CDS             complement(8293..8919)
                     /gene="pcm"
                     /locus_tag="POB34_003008"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HDI5919911.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(8913..9674)
                     /gene="surE"
                     /locus_tag="POB34_003009"
     CDS             complement(8913..9674)
                     /gene="surE"
                     /locus_tag="POB34_003009"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HDI5919912.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(9655..10704)
                     /gene="truD"
                     /locus_tag="POB34_003010"
     CDS             complement(9655..10704)
                     /gene="truD"
                     /locus_tag="POB34_003010"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HDI5919913.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(10701..11180)
                     /gene="ispF"
                     /locus_tag="POB34_003011"
     CDS             complement(10701..11180)
                     /gene="ispF"
                     /locus_tag="POB34_003011"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDI5919914.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11180..11890)
                     /gene="ispD"
                     /locus_tag="POB34_003012"
     CDS             complement(11180..11890)
                     /gene="ispD"
                     /locus_tag="POB34_003012"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDI5919915.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(11909..12220)
                     /gene="ftsB"
                     /locus_tag="POB34_003013"
     CDS             complement(11909..12220)
                     /gene="ftsB"
                     /locus_tag="POB34_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HDI5919916.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(12414..12737)
                     /locus_tag="POB34_003014"
     CDS             complement(12414..12737)
                     /locus_tag="POB34_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HDI5919917.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(12787..13392)
                     /gene="cysC"
                     /locus_tag="POB34_003015"
     CDS             complement(12787..13392)
                     /gene="cysC"
                     /locus_tag="POB34_003015"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HDI5919918.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13392..14819)
                     /gene="cysN"
                     /locus_tag="POB34_003016"
     CDS             complement(13392..14819)
                     /gene="cysN"
                     /locus_tag="POB34_003016"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HDI5919919.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEKTFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMAWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(14821..15729)
                     /gene="cysD"
                     /locus_tag="POB34_003017"
     CDS             complement(14821..15729)
                     /gene="cysD"
                     /locus_tag="POB34_003017"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HDI5919920.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
BASE COUNT         3706 a         4349 c         4039 g         3910 t
ORIGIN      
        1 catatttcgt cgcatcacag accaaaactc gctggcggct ggcactggca atcgcccgtt
       61 tcaccgtgac tttatcttcc gctggcgtag aaatcccccg cacactccat gacgatgcag
      121 aaataaaagc ctgatcaatc atcaggctgc gcagcatggt cgcagcggct tccccgacac
      181 aggaacggtt ttcccgacac actgcaccgc cagtgtgaat aattgtgcaa ttactgttgt
      241 cgagcaagta gttcgcaata acgaaatcgt ttgtgaccac agtcagtgac tccatgtgaa
      301 tcagatgctg tgctatcgct aacgtggtcg ttcccgcatc cagatagata cagcttcccg
      361 gctgaacaag acttgccgcc agcttgccaa tagccgcttt ttgcgtcatt gccagcgcag
      421 tttttacctg atgagaaggt tcatgcgcca cgcgtcccgg agactggacg cctccggaca
      481 ccagcacaac ggctccctgc tgctccagtt tttgtaaatc ccgacgaatg gtcatatgtg
      541 acacattcat tctgtccgtt agttcagcaa tactgacaat gcctttttca gctaccatct
      601 caaggatgat ttggcgacgc tctacgggta tcaacttttg ctccttcctt tgtcctgctg
      661 acattctacg ctatttgcct gcgaaacgtg cgcggcgcaa ctaacgctta gttcacataa
      721 aataacacac aatgttaatt tatgtgaatc agctcaccat atcgttatct tccagcgctt
      781 atattcacaa tatcaaacaa aatatcactt aaattaacaa ggagagcaga tgaaaacggg
      841 atctgagttt catgtcggta tcgttggctt agggtcaatg ggaatgggag cagcactgtc
      901 atgtgtccgc gcaggtcttt ctacctgggg cgcagacctg aacagcaatg cctgcgctac
      961 gttgaaagag gcaggtgctt gcggggtttc tgataacgcc gcgacgtttg ccgaaaaact
     1021 ggacgctctg ctggtgctgg tggtcaatgc ggcccaggtt aaacaggtgc tgtttggtga
     1081 aacaggcgtt gcacaacatc tgaaacccgg tacggcagta atggtttctt ccactatcgc
     1141 tagtgctgat gcgcaagaaa ttgctaccgc tctggctgga ttcgatctgg aaatgctgga
     1201 tgcgccagtt tctggtggtg cagtaaaagc cgctaacggt gaaatgactg tcatggcctc
     1261 cggtagcgat attgcctttg aacgactggc acccgtgctg gaagccgttg ccggaaaagt
     1321 ttatcgcata ggtgcagaac cgggactagg ttcgaccgta aaaattattc accagttgtt
     1381 agcgggcgta catattgctg ccggagccga agcgatggca cttgcagccc gtgcggggat
     1441 cccgctggat gtgatgtatg acgtcgtgac caatgccgcc ggaaattcct ggatgttcga
     1501 aaaccggatg cgtcatgtgg tggatggcga ttacaccccg cattcagccg tcgatatttt
     1561 tgttaaggat cttggtctgg ttgccgatac agccaaagcc ctgcacttcc cgctgccatt
     1621 ggcctcaaca gcattgaata tgttcaccag cgccagtaac gcaggttacg ggaaagaaga
     1681 cgatagcgca gttatcaaga ttttctctgg catcactcta ccgggagcga aatcatgatc
     1741 aagattggcg ttatcgccga tgattttacc ggcgcgacgg atatcgccag ttttctggtg
     1801 gaaaacggtc taccaacggt acaaattaac ggtgttccaa caggtaaaat gccggaagca
     1861 atcgacgcac tggtgatcag cctgaaaacg cgctcctgtc cggtggttga agccacacag
     1921 caatcgctgg cggctctgag ctggttgcaa cagcaaggtt gcaaacagat ctatttcaaa
     1981 tactgctcta ctttcgacag tacggcgaaa ggtaatatcg gcccggttac cgatgcatta
     2041 atggatgctc tcgacacgcc gtttacggtc ttctctccgg ccctgccggt caacggacgt
     2101 acggtttatc aggggtattt gttcgtaatg aatcaactgc tggccgaatc cgggatgcgc
     2161 catcacccgg taaatcccat gaccgacagc tatcttcccc gtctggttga agcgcaatcc
     2221 acagggcgct gcggcgtcgt ttcggcacat gttttcgaac aaggtgtgga tgccgttcgt
     2281 caagagctgg ctcgcttaca gcaagagggc taccgctacg cggtgcttga tgcgctgacc
     2341 gaacaccatc tggaaattca gggagaagcc ttgcgcgatg ccccactggt aacgggcggt
     2401 tctggtctgg cgattggcct ggcccggcag tgggcgcaag aaaacggtaa ccaggctcgc
     2461 gaagcagggc atccgctcgc tgggcgcggc gtagtgctct ccggttcatg ctctcaaatg
     2521 accaaccgcc aggtggcaca ttaccgtcaa attgcaccag cccgtgaagt tgatgtggca
     2581 cgctgcctct caaccgaaac tctggccgct tatgcacacg aactggcaga gtgggttctg
     2641 ggccaggaaa gtgtacttgc tccactggtt tttgccaccg ccagcactga cgcattggca
     2701 gcaattcaac agcaatacgg tgcacaaaaa gccagtcagg cagtagaaac tctgttttct
     2761 caactagcgg cgcggttagc agcggaaggc gtgacacgct ttattgtcgc aggcggtgag
     2821 acctccggcg tagtcacaca gagcctgggc ataaaagggt ttcatattgg cccaaccatt
     2881 tcccccggcg tgccgtgggt aaacgcactg gataagcctg tctcactcgc ccttaaatct
     2941 ggcaacttcg gtgatgaagc cttttttcac gagcccaaag agagttttta tcatgagcga
     3001 tttcgcaaaa gtagagcagt ctttgcgaga ggagatgacg cggattgcca gttcattctt
     3061 tcagcgcggc tatgcaaccg gttcggctgg caatctgtcg ctgcttttac ctgacgggaa
     3121 tttactggcg acaccgacag gttcatgcct gggcaatctc gatccgcagc ggctttccaa
     3181 agtcgccgcg gatggcgaat ggttaagtgg tgacaaaccc tcgaaagagg tgctctttca
     3241 tctggcgctg tatcgcaaca atccgcgctg taaagcggtg gtgcatttgc acagcacatg
     3301 gtcgacggcg ctttcctgcc tgcaagggct ggacagcagc aacgttattc gtccgttcac
     3361 accatacgtg gtgatgcgga tgggaaatgt cccgctggtg ccttattacc gaccgggcga
     3421 taaacgcatc gcacaggatc tggcggaact ggcagcagac aatcaggctt ttttactggc
     3481 aaatcatggc ccagtggttt gcggtgaaag cctgcaagaa gccgccaaca atatggaaga
     3541 gctggaggaa acggcaaagc tgatttttat tctcggtgac cgcccgatcc gttatctgac
     3601 cgcaggtgaa attgcggaat taaggagtta atgcaatgcc tcgttttgca gctaatttat
     3661 ccatgatgtt caccgaagtg ccttttattg aacgcttcgc cgcagcgcga aaagccgggt
     3721 tcgatgctgt ggaatttctg tttccctatg actactccac cctgcaaatc caaaagcaac
     3781 tggagcaaaa ccatctgaca ctggcgctgt tcaataccgc tcccggagat attaatgcgg
     3841 gggaatgggg attatccgcc cttcccggac gtgagcacga agcacacgca gacatcgacc
     3901 tggcactgga atatgcattg gcgctcaatt gtgaacaagt ccatgtgatg gcaggcgtcg
     3961 tgcccgctgg cgaagacgct gaacggtacc gggcagtatt tatcgataat ctccgctacg
     4021 ctgccgaccg ctttgcgcta cacggcaagc gaatattagt tgaagcactc agccccgacg
     4081 ttaagcctca ttatctcttc tccagccagt atcaggcact ggctattgtt gaagaggttg
     4141 cgcgagataa cgtgtttatt caactagata cttttcatgc gcaaaaagta gatggcaacc
     4201 tgacgcattt aatccgcgac tacgccggga aatatgcgca cgtacaaatt gccggactac
     4261 ctgatcgaca tgaaccggac gatggagaaa tcaactatcc gtggctgttc cgcctgttcg
     4321 atgaggtggg atatcagggc tggatcggtt gtgaatataa acctcgtggc ctcaccgaag
     4381 aagggcttgg ctggtttgac gcctggcgct aattcgttaa atccctaatt acaacgtacc
     4441 catacatccc cccatctacg gatggggtag gttaactata actttcagac agggtttccc
     4501 atgtccacaa ttacattgtt atgcattgcg ttaactggcg taatcatgct gttgctgctg
     4561 gtcatcaagg caaaggtaca accattcgtt gctctgctcc tcgtcagcct gttagtcgca
     4621 cttgcggcgg gtataccggc gggcgaagtg ggtaaagtga tgatcgccgg gatgggcggt
     4681 gtgcttggtt ccgtcactat tattattggt ctgggcgcta tgctggggcg tatgatcgaa
     4741 cactctggtg gtgcagagtc actggctaat tatttcagtc gcaagttagg tgacaaacga
     4801 actatcgctg cgctgactct ggcagcgttc ttcctcggta ttcccgtctt ctttgatgtc
     4861 ggctttatta ttcttgcgcc aatcatttac ggttttgcca aggttgccaa aatatcgcca
     4921 ctcaaatttg gcctgcctgt cgctgggatc atgctaactg tacatgtggc ggtaccgccg
     4981 catccaggcc ctgtcgccgc agcggggtta ctccacgcag acatcggctg gctaaccatc
     5041 atcggtattg cgatttctat tcccgtaggg attgttggct actttgcagc gaaaataatc
     5101 aataagcacc aatatgcgat gtcggtggaa gtactggaac agatgcaact ggctccggcc
     5161 agtgaggaag gcgcgacaaa attaagcgat aaaataaatc caccgggcgt cgcgctggtc
     5221 acctcgctaa ttgttattcc tatcgcgatt atcatggcgg gtacggtttc cgcaacactg
     5281 atgccgcctt cgcatcccct gcttggtacg ctacagctga tcggctcacc aatggtagct
     5341 ctaatgattg cgctggtgct ggcattctgg ttattggctt tacgtcgcgg ctggagctta
     5401 caacacacca gcgacattat gggctcagcc cttcccactg cggcggtagt gattttggtt
     5461 actggtgctg gaggggtatt tggcaaagtg ctggtggaat cgggcgtcgg caaagccctt
     5521 gccaatatgc tacaaatgat tgacctgcct ctgttaccag ccgcatttat tatttcactg
     5581 gctctgcgtg catcgcaggg gtcagcaacc gtagcaatcc tgacaaccgg cgggttactc
     5641 tcagaagcgg tgatgggatt gaatccgatt cagtgcgtat tggtgacgct ggcagcctgc
     5701 tttggtgggc ttggcgcgtc acatattaat gactcagggt tctggattgt gaccaaatat
     5761 ctggggttgt cggtagcaga cggtctgaaa acctggacag tgttaacgac cattctcggt
     5821 tttaccggat tcttaattac ctggtgcgta tgggcggtaa tttgacctta ataaaaaggt
     5881 ccgatgggca tcggaccttt tattgtgcac agaaaaggcc agcctcgctt gagactggcc
     5941 tttctgacag atgcttactt actcgcggaa cagcgcttcg atattcagcc cctgcgtttg
     6001 caggatttca cgcaaacggc gcaggccttc aacctgaatc tggcgaacac gttcacgggt
     6061 gaggccaatt tcacgaccta catcttccag tgttgccgct tcgtacccca gcaaaccgaa
     6121 tcgacgtgcc agcacttcac gctgtttggc gttcagctcg aacagccatt tgacgatgct
     6181 ctgcttcata tcgtcatctt gcgtggtatc ttccggaccg ttctcttttt catcggccag
     6241 gatgtccagc aacgcttttt cggaatcacc acccagcggg gtgtctaccg aggtaatgcg
     6301 ctcgttaaga cgaagcatac ggctgacgtc atcaactggc ttatccagtt gctctgcgat
     6361 ctcttccgca cttggttcat ggtccagctt atgggacaac tcacgtgcgg ttcgcaggta
     6421 aacgttcagc tcctttacga tgtgaatcgg caaacgaata gtacgggttt ggttcataat
     6481 cgcccgttca atcgtctggc gaatccacca ggttgcgtat gttgagaagc ggaaaccacg
     6541 ttccgggtca aacttctcta ccgcgcggat cagccccagg ttgccctctt cgataaggtc
     6601 cagcaacgcc agaccacgat tgccataacg gcgggcaatt tttaccacca gacgcaagtt
     6661 actctcgatc atccggcggc gagaggcgac atctccacgc agtgcgcgac gcgcaaaata
     6721 aacttcttct tcggccgtta acagtggtga ataaccaatc tcaccaaggt aaagctgagt
     6781 cgcgtccaac acacgctgtg tggctccctg cgataacagt tcctcttcgg ccaaatcgtt
     6841 atcactgggt tcctcttcta ctaaggcctt ttcgtcaaaa acctcaactc cgttctcatc
     6901 aaattccgca tcttcattta aatcatgaac tttcagcgta ttctgactca taaggtggct
     6961 cctacccgtg atcccttgac ggaacattca agcaaaagcc tggttccgtc gatttatcgc
     7021 tgcggcaaat aacgcagcgg gtttacggat ttccccttgt aacgaatttc aaaatgcaag
     7081 cgtgttgaac tggttccggt gctacccatg gtcgctattt tttgccccgc cttaacttct
     7141 tgttgttccc ggaccagcat tgtgtcgtta tgggcgtagg cactcaggta atcatcatta
     7201 tgtttgatga taatcagatt accgtagccg cgcagcgcgt taccagcata aacaacgcgg
     7261 ccatctgcgg tcgcgataat tgcctgtcct ttgctgcctg cgatatcaat ccccttgttg
     7321 cccccctcag aagcgccaaa ggtttcgatc actttgccct cagtcggcca gcgccaggtg
     7381 gagataggcg tactggttga tgtactgctg acagtcggct cggttgtgct tgctgttggt
     7441 accgttacag gcgctgtgac cgtggtcgca gttggcttgt tgttcggcaa cattttgtta
     7501 gcactctgtt cacccgaaga ctcagaatac gtaattgtcg gttgcgacgc aacagcaacg
     7561 gtggaatttt gtgcaggctt gatcacaact ccttgctctg ctgcgtcggc ctgggtaatg
     7621 gcatttccgc cagtgattgg cgtaccggaa gcattaccca cctgcaaggt ctgaccaacg
     7681 ttcagcgcgt atggtgcctg aatattgttg cgctgagcaa ggtcacggaa atcgttgcca
     7741 gtaatccagg cgatatagaa aagtgtgtcg ccttttttca cggtataggt actgccgcta
     7801 taactgcctt tcggaatgtt cccatactga cggttataga cgatgcgtcc gttttccatc
     7861 tgtaccggct gctgagctac tggctgcact ggctggattt gcggttgttg agtagcctga
     7921 atttgtggct gctgcaccgg ctgaatttgc ggttgctgcg ctgtagacgt cgtccccatt
     7981 ttcggcggcg gcgtaatcaa cataccagaa ttagtatttg caggcgcatt gccattaacg
     8041 gagctgaccg gtgccggtgg atttgaagtg tcagaacagc ctgccagcca tagcgaaacc
     8101 agtgacaaag ccgcaatgcg gcgaacggtg aattttgggc ttcccgcgct catttatccc
     8161 ccaggaaaaa ttggttaata accagtgaca taattaccgt gcaaggcacc ctactgaaca
     8221 ctggaaaaga tgttcacgat acgctgacct gcggcaaaat aaccaggaaa aatccaggta
     8281 tttcctcacg ttttaagcca gctcaccctt cactaaaggg acaaagcgca cggcctccac
     8341 ggtatcgata ataaattcgc ctccccgacg acgcacccgt ttcaaatact ggtgctcctc
     8401 ccctacgggt aagacgagaa tcccgccttc gtccagctgc gtcattagcg cagttggaat
     8461 ttccggcggt gccgccgtaa caatgatagc gtcaaacggc gcacgtgcct gccaaccttg
     8521 ccatccatcg ccatgacggg ttgaaacatt atgtaaatca agatttttca ggcggcgacg
     8581 tgcctgccac tgcaagcctt taatccgttc aaccgagcaa acatgctgga caagatgcgc
     8641 caggattgcc gtttgatatc ccgaaccggt gccaatttcc agcacccgcg actgcggcgt
     8701 cagctcgagt aattcggtca ttcgcgccac catatatggc tgcgaaattg tctgcccctg
     8761 acctatcggc aaagcgatat tgtcccaggc tttttgttca aacgcttcat caacgaattt
     8821 ttcacgcggc acggcggcaa gtgcattcag cacctgctca tcctgaatac cttgcgcacg
     8881 taattgatcc agaagtgctt gtacgcgtct gcttaccatt gcgtgccaac tcccacgctg
     8941 tttaaccagt ctgaaaccac atcttgcgcg ctatgcgcag ttaaatccac atgcagcggc
     9001 gtgatggaga catagccctc atctaccgca gcaaaatcgg tccccggacc agcatcacat
     9061 ttaccgcccg gcgggccaat ccagtacagc gtattgccgc gcggatcttg ctgcgggatc
     9121 acctgatctg ccggatgtcg tgtaccgcag cgcgtcacgc gaataccttt gatttgatcc
     9181 aagggtaaat ccggaacgtt aatattaaga atacgcccgg tgcgcagcgg ctctttacac
     9241 agtgcgcgca aaattgaaca ggttaccgcc gcggcagtgt cgtaatgttt atgcccgtca
     9301 agcgagacgg caagcgccgg aaaacctaaa tgacggcctt ccatcgcggc ggctaccgta
     9361 ccggaataaa taacatcatc ccccagattc ggcccggcgt taattccgga cacaacaatg
     9421 tccgggcgcg gacgcatcag agcattcacg ccaagataga cgcaatcggt cggggttccc
     9481 atttgcacag caatatcacc attttcaaag gtaaacgtgc gcagggagga ttccagtgtc
     9541 agagaatttg aagcgccgct gcggttacga tcgggggcga ccacctgaac gtcagcaaac
     9601 tcacgcaagg ctttcgccag cgtttgtata ccgggtgcat gtaccccgtc atcattactc
     9661 agcaatatgc gcataatcac ctgttgtgtt gataagttcc ctgacaacgc tggttgcaaa
     9721 actacccgcc ggaagccaga aacggatctc tacggtgacg tcatcccacc aattccagct
     9781 taattgttgc ggatacagca gcatcgctct gcgcgcggct tcaacttttt cgcgcaccag
     9841 taaagcttgt aattcagttt ctgcggcgac agctgcttgt tcgaatgcca gcgcttcacg
     9901 ctgagttccc cattcgccac tgcctggcaa tgcggcggtt atcatcaact ctttatcgtt
     9961 gacgcgacgc tgtaattccg ccagttcttc ggtggttgct acaaaccagc taccacgtcc
    10021 ggctaattgt agcgcatcgc cgtcaacaac ttgattaacg tctgcttttt tgaggcgctc
    10081 agcaacaatc tgattaaaca acgcactgcg ggctgccgac aaccaaaaac tccgtttatt
    10141 gcgatcgcgc accggagtat tggtttgcgc ccagcgctgc gccccctgca agttgctacc
    10201 gccaatccca aaacgttggg caccgaagta gttcggtaca ccttttacgc aaatatcgat
    10261 cagacgttgt tcaacgtcat cgcgattgct cacttcgcgc agaaccaggg taaaggcgtt
    10321 tcctttcagc gcccctaaac gcagcttgcg cttgtgccgc gcatactcca gcacctggca
    10381 gccttccagt tgaaaggcgc tcagatcggg catttccttg cccggcacgc gagcgcataa
    10441 ccactgttcc gtaacagcat gtttgtcttt ttgcccagcg aagctgactt cacgggcatg
    10501 aattttcagg aatttcgcca gtgcatccgc cacaaaacgg gtattgcagc cgtttttgag
    10561 gattctaacc agaatatgct caccttcacc atcaggctca aagcccaaat cttccaccac
    10621 cacaaagtct tccggattgg ctttcagcag cccggtgcct tgcggtttac cgtggaggta
    10681 agtgagatta tcaaactcaa tcattttgtt gccttaatga gtagcgccac cgcttcacag
    10741 gcaatccctt ccccacgtcc ggtaaatccc agtttttccg tagtagtggc tttcacgtta
    10801 acatcatcca tatggcagcc gagatcttcg gcaataaaca cgcgcatttg tggaatgtgc
    10861 ggcaacatct tcggtgcctg agcgatgata gtgacatcga cgttgccaag ggtataaccc
    10921 ttcgcctgaa tacgacgcca ggcttcgcgt agcagctcgc ggctatcggc acctttaaat
    10981 gccggatcgg tatccgggaa cagcttgccg atatccccca gcgccgccgc gccaagcaat
    11041 gcatcggtca acgcatggag cgccacgtcg ccatcagaat gcgccagcaa tcctttttcg
    11101 taaggaatgc gtacgccacc aatgataatt gggccttcac cgccaaaggc atgtacgtca
    11161 aaaccatgtc caattcgcat tatgtattct cctgatggat ggttcgggtg aggtaaaact
    11221 cggccagtgc caaatcttcc gggcgcgtga ctttaatgtt atccgcacgg ccttcgacca
    11281 actgaggatg gaatccgcaa tattccagcg ccgaggcttc gtcggtaata gtcgcgcctt
    11341 catttagagc gcgcgtcaga cagtcatgta acagctcacg agggaaaaat tgcggcgtca
    11401 gcgcgtgcca taagccgttg cgatcaacgg tatgagcaat ggcatttttg cccggttcgg
    11461 cacgtttcat agtatcgcgc actggtgcgg cgaggatccc ccccgtgcgg ctggtttcgc
    11521 tcaacgccaa caatcgcgcg aggtcatcct gatgcaaaca aggacgagcg gcgtcatgca
    11581 ccaataccca ctgcgcgtcg ccagcggctt tcagacctgc cagcacggaa tcggcacgct
    11641 catcaccgcc atctacaacg gtgatttgcg gatgattcgc cagaggaagt tgtgcaaaac
    11701 ggctatcgcc aggacttatg gcaatgacga ctcgtttcac ccggggatgc gccagcagcg
    11761 catgcaccga gtgttcaaga atggtttgat taccgattga gagatattgc ttaggacatt
    11821 ccgtttgcat tcgacggcca aatccggccg ccggaaccac ggcgcaaaca tccaaatgag
    11881 tggttgccat gttaattccc gggctgattt atcgattgtt ttgccccgca gactgtgcgc
    11941 gcttcgacgc gtcaggcacc agacgataaa aagtttcgcc cggcctggtc atgctgagtt
    12001 cattacgcgc acgctcttcg agcgcctcct ggccgccatt gagatcgtca atttcggcaa
    12061 aaagttgatc gtttcgcgct ttaagtttcg cgtttgtagc ttgctgtgcc gccacatcat
    12121 cattgacgcg ggtatagtca tgtataccgt tcttaccgaa ccacagcgaa tactgtagcc
    12181 agaccagaat agccagcaac agcagcgtta gtttacccat cctgccccct gaaaaacggc
    12241 atcatcatcc catgcatccg aagacgactc tacatcctct gttggggata ccgcgacaac
    12301 gcgggcaaat gtaccacatt tgtccattgt tacgtatacc cagggcgtgc agaacataat
    12361 ctcattatta gttacggttt gaattatgaa cagaggagac gggaaagtac aaattagccc
    12421 agtagccaca taaacagtgc gccaaacata atgcctactg tcaccagggt gaaaacaata
    12481 ctgtagcgta gctttccgtc catcaatgaa tgcagcgcaa tccccaccac taccgcgacg
    12541 ggcatcagcg ccagaaagaa aggccaggtg tagataaaga agaacagcgt gttagagcca
    12601 taaatcaaca tcggcatcgc cagcgcaaat aaccaggaga taaaaccgac cacggcacca
    12661 ggcagtgacc atgtggtttc ttcatcctca gtaaggctgt cgttatttgt tagtgtaatg
    12721 ttatggctat tacgcatatt tgatcctgtt actttgacga accgggcatg gaaacccggt
    12781 ggtgtctcag gatctgataa tatcgttctg tctcaacaga tctaataatt gctgtaccaa
    12841 atttgttact aattgttcac cattgagatg aatttctgcc gattcaggcg cttcgtaaac
    12901 ggaatctatt cccgtaaagt tgcgcagttc accggcacgc gctttcttat ataagccttt
    12961 tggatcgcgg gcttcgcaaa tcgccagcgg cgtatcgaca aacacttcaa taaagcgccc
    13021 ttctcctacg cgttcgcgaa ccatctggcg ttcggcgcgg tgtggcgaga taaatgcggt
    13081 cagcaccacc agtccggctt caaccatcaa attcgccact tcaccgacgc gacggatatt
    13141 ctctttacga tcggcatcgc taaaaccgag atcgctgcat aatccgtggc gaacattatc
    13201 gccatccagc agatacgtac tgacgccgag tttatgtaac gcctcctcca gcgccccggc
    13261 gaccgttgat ttaccggacc cggagaggcc ggtaaaccac agcactacac cacgatgacc
    13321 gtggtgtagc tcgcgttgtt gcacagtgac cggatggcta tgccagacga cgttttcgtc
    13381 atgcagcgcc attatttatc ccccagcaaa tcgcgcgcgc cccagtgtgg aaagtggcgg
    13441 cgaaccaggg cattcaattc caattcgaat gcactaaatt cagatggcgc agcagttgcc
    13501 tggctaactg gctcgtgcac cataccggcg cccacggtca cattgctaag gcgatcgata
    13561 aaaatcagcc caccggtaac cggattttgc tgataacgat ctaacaccag tggctcgtca
    13621 aaagtgagat ccaccaggcc gatgccgttc agcggcaggt tttcaacttc gcgttgggta
    13681 aggttattaa tatcaacctg ataacgaatg ccatcaacac gagcacgcgt cttcttaccg
    13741 gcaattttga tgtcataact ctggcccggg gaaagcggct gttccgccat ccataccaca
    13801 tccaccgacg cgctctgcac agctggtaac gcttcgtctg ccgccagcag cagatcgcca
    13861 cggctgatgt cgatctcatc cgtcagcacc agggtgatcg cttctccggc aaaggcttct
    13921 tcgcgatcgc catcaaaagt cacgatccgg gcgacgtttg attccacacc agagggcagc
    13981 actttgactc gttgcccgac ttctacgcga ccggatgcca gcgttccggc gtaaccacga
    14041 aaatcgagat tcgggcgatt aacgtactgc accgggaagc gcattggctg agcatccacc
    14101 actcgctgga tctccacggt ttccagcact tcgagcagtg ttggaccgct gtaccacgcc
    14161 atactttcac tttgcgaagc cacgttgtcg ccttccagtg cagagagcgg cacaaagcgg
    14221 atatccagat tacccggcag ctgcccggca aaggtcagat aatcttcacg aatacgggtg
    14281 aacgtctttt cactgtaatc caccagatcc attttgttga tcgccacgac cagatgtttg
    14341 atccccaaca gtgtggagat aaaactgtga cgacgggttt gatcgagcac gcctttacgg
    14401 gcatcgatca gtaagatcgc cagttcacat gtcgatgcgc cagtcgccat attgcgggtg
    14461 tactgctcgt gccctggggt gtcggcgata ataaatttac gcttctcggt agagaaatag
    14521 cggtaagcca cgtcaatggt gatgccctgt tcgcgctcag cttgcaggcc gtccaccagc
    14581 agtgccagat ccagcttttc gccctgggtg ccgtgacgtt tactgtcgtt atgcagcgat
    14641 gagagctgat cttcgtagat ttggcgggta tcgtgcagca ggcgaccaat cagggtactt
    14701 ttgccgtcat cgacgctacc acaggtcaga aaacgcagca ggcttttatg ttgttgcgca
    14761 atcatccagg cttcgacgcc gccttcattg gcgatttgtt gtgcaagtgc ggtgttcatc
    14821 ttaaaaatac ccctgacgtt ttttcagctc catagacccc gcctggtcgc ggtcaatcac
    14881 gcggccctga cgttcactgg tggtagaaac cagcatctct tcgatgatct ccggtagtgt
    14941 ttgtgcattt gactccaccg caccggtcag cggccagcag cccagcgtac ggaaacgcac
    15001 catccgtttt ttaatcactt cgcccggttg caggtcgata cggttgtcat caatcatcat
    15061 caacataccg tcgcgttcca gaaccggacg ttccgcagcg agatacagcg gcacaatgtc
    15121 gatattttcc agccagatgt attgccagat atcctgctcg gtccagttag agagcgggaa
    15181 gacgcggatg ctttcgcctt tgttaatttg cccgttgtag ttgtgccaca gctctgggcg
    15241 ctgatttttc ggatcccagc gatggaagcg gtcacggaaa gagtagattc gctctttagc
    15301 gcgggatttc tcttcgtcac ggcgcgcacc accgaaggcg gcatcaaaac cgtatttgtt
    15361 cagcgcctgt ttcaggcctt cagttttcat aatatcggta tgtttcgcgc tgccgtgcac
    15421 gaatggatta atccccatcg ccacgccttc cgggttttta tgcaccagca gctcgcagcc
    15481 gtaggctttc gccgtacgat cgcggaactc atacatctcg cggaatttcc agccggtatc
    15541 gacatgcagc aacgggaaag gcagcgtacc tggataaaac gccttgcgcg ccagatgcag
    15601 catgacgctg gaatctttac cgatagagta gagcatcacc ggatttgaga attctgccgc
    15661 cacctcgcga ataatgtgga tgctttccgc ctccagttgc cgcaggtgag taagtcgtat
    15721 ttgatccata accgttcctt tgcaataccg ctattttctt gccatcagat gtttcgacta
    15781 tagggagcgt aagagaacga atgaaattac caattagaat gagtagttcc ttaacggaat
    15841 aacgatttgg caaagctaat atcaaaaagt gcttaaggca ccggatttcg ggcgtttagg
    15901 aagatttgaa attgttttag cgcagcggca gtttcatact atggcggtaa aaaaatttgc
    15961 atggtattta aggactcact atgttttccg cattgcgcca ccgt
//
LOCUS       DAMZET010000093        15821 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.093, whole genome shotgun sequence.
ACCESSION   DAMZET010000093 DAMZET010000000
VERSION     DAMZET010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.093"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1138
                     /gene="menE"
                     /locus_tag="POB34_003018"
     CDS             <1..1138
                     /gene="menE"
                     /locus_tag="POB34_003018"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDI5919921.1"
                     /translation="QCGARVLPVNPQLPQPLLEELLPNLTLQFALVPDGENTFPALTS
                     LHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHLASAEGVLSLIPFGDH
                     DDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLV
                     NRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGS
                     PLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYATRDRGEMHNGKLTIVG
                     RLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVMEYDHESVDL
                     SEWVKDKLASFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQQ"
     gene            1248..1514
                     /gene="pmrD"
                     /locus_tag="POB34_003019"
     CDS             1248..1514
                     /gene="pmrD"
                     /locus_tag="POB34_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HDI5919922.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(1508..1894)
                     /gene="arnF"
                     /locus_tag="POB34_003020"
     CDS             complement(1508..1894)
                     /gene="arnF"
                     /locus_tag="POB34_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HDI5919923.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPMTKQRY"
     gene            complement(1894..2229)
                     /gene="arnE"
                     /locus_tag="POB34_003021"
     CDS             complement(1894..2229)
                     /gene="arnE"
                     /locus_tag="POB34_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HDI5919924.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            complement(2226..3878)
                     /gene="arnT"
                     /locus_tag="POB34_003022"
     CDS             complement(2226..3878)
                     /gene="arnT"
                     /locus_tag="POB34_003022"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HDI5919925.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPNFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFEIYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            complement(3878..4768)
                     /locus_tag="POB34_003023"
     CDS             complement(3878..4768)
                     /locus_tag="POB34_003023"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HDI5919926.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            complement(4765..6747)
                     /gene="arnA"
                     /locus_tag="POB34_003024"
     CDS             complement(4765..6747)
                     /gene="arnA"
                     /locus_tag="POB34_003024"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HDI5919927.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFTYVGNQKFTVWSSRVHPHASKAQPGSVISIAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLLIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            complement(6747..7715)
                     /gene="arnC"
                     /locus_tag="POB34_003025"
     CDS             complement(6747..7715)
                     /gene="arnC"
                     /locus_tag="POB34_003025"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HDI5919928.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            complement(7719..8858)
                     /gene="arnB"
                     /locus_tag="POB34_003026"
     CDS             complement(7719..8858)
                     /gene="arnB"
                     /locus_tag="POB34_003026"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HDI5919929.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPDWPHVHAWHLFIIRVDEQCCGISRNALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            9166..9768
                     /locus_tag="POB34_003027"
     CDS             9166..9768
                     /locus_tag="POB34_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDI5919930.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(9807..10232)
                     /gene="nudI"
                     /locus_tag="POB34_003028"
     CDS             complement(9807..10232)
                     /gene="nudI"
                     /locus_tag="POB34_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HDI5919931.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            10511..11053
                     /locus_tag="POB34_003029"
     CDS             10511..11053
                     /locus_tag="POB34_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919932.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            11153..12355
                     /locus_tag="POB34_003030"
     CDS             11153..12355
                     /locus_tag="POB34_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HDI5919933.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            12410..12880
                     /locus_tag="POB34_003031"
     CDS             12410..12880
                     /locus_tag="POB34_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDI5919934.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(12921..13823)
                     /locus_tag="POB34_003032"
     CDS             complement(12921..13823)
                     /locus_tag="POB34_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HDI5919935.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(14017..15207)
                     /gene="glpC"
                     /locus_tag="POB34_003033"
     CDS             complement(14017..15207)
                     /gene="glpC"
                     /locus_tag="POB34_003033"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HDI5919936.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVYTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYTTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(15204..>15821)
                     /gene="glpB"
                     /locus_tag="POB34_003034"
     CDS             complement(15204..>15821)
                     /gene="glpB"
                     /locus_tag="POB34_003034"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HDI5919937.1"
                     /translation="CEMILMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRL
                     QNQLQRQFVRQGGVWMPGDEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFS
                     GGLVAERNGIREPILGLDVLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQT
                     IENLFAIGSVLGGFDPIAQGCGGGVCAVSALHAAQQIAQRAGGQQ"
BASE COUNT         3897 a         3972 c         4084 g         3868 t
ORIGIN      
        1 gcaatgcggg gcgcgggtgt tgcccgtgaa ccctcagctg ccgcaaccgt tgcttgaaga
       61 attgctgccc aatctgacgc tgcaatttgc gctggtgccg gatggggaaa acacgtttcc
      121 ggcgttaacg tcgctgcaca ttcagcgggt tgaaggcgca catgccgcta cgtggcagcc
      181 gacgcgtctg tgctcaatga cgttgacctc gggttctacc gggttgccga aagccgctgt
      241 ccatacttat caggcccatc ttgccagtgc cgaaggggtg ttatcgttga ttccgtttgg
      301 cgatcacgat gactggttgc tctctttacc gctgtttcac gtctccggtc agggaattat
      361 gtggcgctgg ttatacgctg gtgcgcggat gacggtgcgt gataaacagc cattggagca
      421 aatgctggca ggctgtactc atgcttcact ggtgccaaca caactctggc gtttgctggt
      481 taaccgcagt tccgtttccc tgaaagcggt gttacttggc ggcgcggcta tcccggtcga
      541 gttgacggaa caggcgcgcg agcaggggat tcgttgcttt tgcggctatg gtctgaccga
      601 gtttgcctcc acggtgtgtg cgaaagaagc cgacggcctg gcagacgttg gttcgccgct
      661 gccgggtcgg gaagtgaaaa tcgttaataa tgaagtgtgg ctgcgggctg ccagtatggc
      721 agaaggttac tggcgtaacg ggcaactggt ttcactggtt aatgacgaag gctggtacgc
      781 tacgcgcgat cgcggtgaga tgcataatgg caagctgacc attgtcggac gtttagacaa
      841 tctattcttc agtggcggag agggtattca gccggaagaa gtcgagcgtg ttattgctgc
      901 acatcctgcg gttttgcagg tgtttatcgt ccccgttgcc gacaaggagt ttggtcatcg
      961 accggtggcg gtgatggagt atgaccacga gagcgttgat cttagtgaat gggtgaaaga
     1021 taagctggcc agttttcagc aaccggtgcg ttggttaacg ttgccgcctg aactgaaaaa
     1081 cggcggtatt aaaatttcac gtcaggcgct aaaagagtgg gtgcaacgtc agcaataatt
     1141 aaccctccgt ttcacttctc atttctgcca ttccattgcc gttgataaag agtttatctg
     1201 ttaatctgta aattgatgtg aaaactctta gcaaacagga taatgcaatg gaatggctgg
     1261 tcaaaaaatc gtgctgcaat aaacaagata acagacatgt acttatgctc tgcgatgctg
     1321 gcggcgcgat aaaaatgatc gccgaagtga agagtgactt cgccgtgaaa gtgggggatt
     1381 tactctcgcc tttgcagaat gcgctttatt gtattaatcg tgaaaagctg catacggtga
     1441 aagtcctctc ggcaagcagt tatagccccg atgagtggga acggcagtgt aaagtggcag
     1501 ggaaaactca gtaacgttgt tttgtcatgg gcaggaaaat cagcatcaac ccgctcataa
     1561 tacaggctac tcccagtagt gctttcagcg aaaaagtccc ttcccagccg ggaagtacca
     1621 ttgaagcaat ccataccagc acataactca tactgagcaa ggcataggct ttgcttagcg
     1681 caagtttatg tagcgtttta taccaacaaa aaacggaaag cagataaccc agcagcccga
     1741 gtaacagtat ccgggcatcc aggccaaaag ctaacagagc cgcgataaaa tcccataagt
     1801 gcgtcatcgg cggcagatga ctcgccgcaa aacccaggct taattgcgca actgaggcaa
     1861 taatgacgct gaataatccc cacatcaggc ccattacacc gtactcccga ggatcacaat
     1921 gccaccaata atgaacgcca ccccacacca gtgacgcggt gataccggtt cgtgccacag
     1981 ttttactgca gccagcgtca cccagacaaa attcagactt aacatcgggt aggcaatgcc
     2041 taccggtacg ttctgcaaga ccagcagcca gagcaccatg gcaagaccaa gacaagccag
     2101 cgccagtccc agccacagca cgatatgttt gcgccgtttg tttatcgcca caaagcaggt
     2161 tgcctgtttc tgacacaact gcccggcaac gctaagcaag ctggcaaaga ctaatgttag
     2221 ccagatcatt tgggacgata ctgaatcagc accagacgct cctgacgatc gatggcatct
     2281 gcgggcggaa tggcgagact gttgatatct tcatcgcggt caaccgaaag cacgagtgta
     2341 ataatcccct cctgacgatg ttgattaagc cagtttgcga actcatcacc gctgacaaat
     2401 ctccctttcg catccggata attaaggccg tatttcaact cacctgtctg gcgatacatg
     2461 atgatgtcat cgcgttgcag gctccacgcc agacctgcgg caacaccgac gctatcagta
     2521 agaatatagc ggcttggctg aagtgattct tgtgtcatct cgacaaaaaa ttgcggatgt
     2581 tttccttcca tgactctgtc aggaattgaa aatcctacca gcaacgccag ccccagcggg
     2641 caaagcgcgg caaaagacca ggtcttttcg acgtttgtta aggtgtacca gccgaaaaat
     2701 gcccatagcg aaaaaatcga ccaggcacaa aagactttat agatctcgaa ggtttgccac
     2761 accggcgtgt tcattggtcc ccagggggag accacaaatg tggcaataat gccagtgacg
     2821 ccaaaagcga tgttaatcca gccattaatc cgcagcgcca gaggattatt ttttgctgcc
     2881 agcaaagcgt aatgcgccat cagcattgcc agagatgcaa agcaggaaag aatataggtg
     2941 ggcaatttac ctttagcgac ggagaaaaac agcagcggca ttatcgtcca gctcaacaaa
     3001 tagacggttg cggaatgctt gcggtttttc cagcctgtgt acagtgcacc ggggagtaat
     3061 cccagccacg gcaggctacc ggcaatgatg accggcacgt agtaccagaa cggagctcta
     3121 tgttgggcat catccagtgc aaagcgttga atatgctcaa cccagaaaaa atagtgccag
     3181 aagttaggct ctcgctgcgc tatcgccagt ccccaaggga gaaccgtcag tacgcaactg
     3241 ataaccgcca gccagccgta aataaagaga tctttccagc gtttttgcgt cgctacccat
     3301 ggcagcacgc ttaataccgg cacggcaagg gcgagaaaac ctttggtcat cacccccatg
     3361 ccgcaggtga ttcccagcag taaaaatcct gcgcttttgc ctttccacgt ctgtgcctgc
     3421 attgccagcc agaagctgca cattcccgcc accagccaga atgcaataaa cggatcgagc
     3481 acggcataag tgccgatggc atagacaata aacaatgaga gataaattac tgtggcgagt
     3541 agagccagac gtttatcgcg ccataagcgc agagtaaacc aggtcaccag cgcggcagtt
     3601 aacagggtcg caaagataac gcctgcccgc acaccaaagt tattcgcgcc aaatagccat
     3661 tgcccaatgc tgttaatcca gtatccggca atgggttttt cgaaataacg tagccctaac
     3721 agatggggca caatccagtc gccggatgcc agcatttctc gactgatttc cgcataacgc
     3781 gtttcatcgg gttgccagag cagacgcgtg ctgatcggta acaggtaata gcaggcaata
     3841 aacgcgaaga ggccgataag gtaacgtacc gatttcatca gcgactaccc gcaatttgtt
     3901 ggcaacccag ccagccttca cgtccggcaa tatttccgcg aacaacttgt ccgagcggca
     3961 acgtctctga taacagttcg cttaaagggc aaaatgtcac gccttcctga gctgcgcgtt
     4021 tgaggagatc cacaaaatta tgctgataag cgcagccttc aacttctgca tgaatggtat
     4081 aaaccggcgt gcctttatct cgcaggatgc ggttgagtaa ccaaccgtta aaatcttctg
     4141 ctttcacatc ccgaccaatc acttcatccc aggtgggtaa ggtcaccgga atttgcgcag
     4201 tgccagggtt tcctgattcg agcaatggac ggaacggcat ggccccacga caatcgctgt
     4261 tgtagcgcaa atggaacgct tcttttgctt cgatcacctt ctggtcggca cgccagcccg
     4321 cagcggcaga acaggttacc ggttgaccga taatctcttc cagagtgcga agaccgcgtg
     4381 caatatcgtc gatcattgtt tgccgatccc agttaccgct acgggcttgc caggcatggt
     4441 gatcccaggc gtgcaggccg acttcgtgat gtttagccgc ttcacgaatg atatcggcat
     4501 tggcatgacc aatctcttta cctggccagg ccgtacctgc cagtaaaata tcccagccat
     4561 aaagcgatgc cgcatttgag cgcagcatct tccacaaaaa ctgtggcttc accagtcgcc
     4621 agagatggcg gcccatattg tccgggccga cgctgaaaaa aatgctggcc tgaatattat
     4681 gcttactcaa gatttccagc agacgcggca cgccttcacg ggtgccacga aaggtatcga
     4741 catcaatgcg taagcctact ttggtcatga tggtttatcc gtaagatcaa cggtgcgcag
     4801 gaagaaatcc agcgtttcgt cgatggtttc ctgcatatca attttcggct cccagtcaag
     4861 gcagcggtgg gcattgcgga tgctcggttt acgatgctct acgtcctgat atcctttgcc
     4921 gtagtagctg ctactttcga caacgcgaaa gcccgcaaac ggtgggaaat gatggcgcag
     4981 cggatgtttt tcgaagctcg ccagcagcat ctcgcccagt tcctcaatgc tcgcttcgtt
     5041 ctcaggattg ccaatgttga taatttcacc gtcgcagcga tttcccgcat tttcgataat
     5101 gcggtataac gcctcgatac catcgcgaat atcagtaaag cagcgttttt gttttccgcc
     5161 atcaatcagc ttaatcggtg aaccttctac cagattgaga atgagttgcg taatagcgcg
     5221 ggagctgcca attcgcgctg cattaaggtt atccagtcgt ggtcccatcc agttaaacgg
     5281 gcggaagagg gtgaactgta aaccctcttt ttcgccatag gcccagataa cccgatcaag
     5341 taattgtttt gacaccgaat aaatccagcg tggtttattc accgggccga cgattaaatt
     5401 agaatggtcc tcatcgaagt atttatcgct acacatccca taaacttctg aagttgacgg
     5461 gaagatgatt cgcttacggt acttcacgca gtagcggata atgagcagat tctcttcaaa
     5521 atcgagttca aatacgcgca gcgggttgcg ggtatattca atcggcgtgg ctatcgccac
     5581 cagcggcaag acgacatcac attttttgac atgatactca atccattcgg aatgaatact
     5641 gatatcgcct tcaacaaagt gaaaatgcgg atgattcaga aaacggctta tcgcatcgct
     5701 gccaatatcc agaccgtaaa cttcataatg atcttcgcgc agcaggcgtt ctgtcagatg
     5761 gttgccaata aagccattca ccccgaggat gagtacccgg gtacggcgtc gggcggtgca
     5821 ggcaggctgg ctattcaagc gtgaaccttg caccaggccc agcgtctgcg ctaattgcga
     5881 gccctgcata gtaatgccgt cgcccgcctg tccggtgacg atttccagcg cgccatcgcc
     5941 acaggcaatc aacagcggtg caatagaaat cacgctcccc ggctgtgctt tgctggcatg
     6001 aggatgaaca cgcgacgacc agacggtaaa tttctgattg ccaacatagg tgaaggcacc
     6061 cggccacgga tcggcaacgg cacgtaccat gttgtgcagt acggatgccg gtttatgcca
     6121 ttcaaggaaa ctgtcatccg gcgttctgcg accaaaacag gtggcttcgt tttcgcgctg
     6181 ggcgatttcc agaatattac cgtgtttaat ggcgggtaat gtctgttcca gtagctggcg
     6241 cgcggcatgg cacaatttat gatgcagcgt aatagcgata tcgtctggcg caatggcaac
     6301 gcgcagttgc gccacaatgg ccccagcatc ggcacgtttt accattcggt gcaatgtaac
     6361 gcccgtttcc gtttcaccgt tcaccagcac ccagttcagc ggcgcgcgac cacgatattt
     6421 tggtaacagc gaaccgtgca gattaaatgc acctgcggga gcgagctgca aaatctcgtc
     6481 gcaaataaga tggcgataat aaaaagagaa aatcacctct ggtgacagtt gggcaatgcg
     6541 ttccacccac agcggatgat taacgttatc cggcgcataa accggaatgc ctctttccgc
     6601 cgccagatga gccaccgaac cataaaaggc tttttcaccg ggattatcgg tatgggtaaa
     6661 aatggcgcta atttcgtaac cggcagccag cagagcttca ataccgaggc atcccatatc
     6721 gtggtaggca aaaacgacgg ttttcattat tcattttcct tgctggatgg acggataact
     6781 tgctgaacaa aatagcgggg gcgggcgcgg acatcggtgt agatcctgcc gatatattca
     6841 ccgagtaatc ccatgccgat aaactgagcg ccaataaaag taaacagcac ggcaaatagc
     6901 ataaagacgc cttctgccgc ccattgtggt ccgaaggtca ggcgtaaaat caccagcagc
     6961 accgcaatgc taaaacctcc aatcgcaata atgctaccga gcagactcag catacgtagc
     7021 ggcgttgtgg taaggcaggt caccaggtcg tacatcaaat taatcaggcg cataaaactg
     7081 tatttggatt caccaaactc acgctcggca tgatgtactg gaatttcaat tgcacggcgg
     7141 gcgaagatat tcgccagaat cgggataaag gtgctgcgtt catggcagtg caacatcgca
     7201 tcgacaatat gacggcgata ggcgcgcagc atacaaccgt agtcacccat cgctttgcca
     7261 gtggtgcgct gaataagccg gttaatcatc ttcgaagcgg ttttacgaaa ccagctgtcc
     7321 tggcggttct ggcgtacagt cccaaccacg tcgtaacctt catcggcttt tgccaccagg
     7381 cgggggattt cttctggcgg attctggaga tcggcatcaa gggtaataat taagtcgcca
     7441 gtaacgtgac tgaatcccgc cataatcgct gaatgttgcc cgtaattgcg gttaagcaaa
     7501 atagacacaa tatggctgtt ctccgcttgt gaggcttcga ccagcatatg cgcggaatta
     7561 tcgctactgc cgtcatcaat cagcaggatc tcatactctt tccccaacga ttcacaggct
     7621 gtggtggtgc gcctgattaa ttccggtaag ctttcctgct cgttataaac gggaataacc
     7681 accgagactt tcttaacagg gtggatttca aacatggctt attgtcctgc gagttgctga
     7741 agggctgtga tgacgcggtc ggcatcggcg gtagtcatat ccgggaacag cggcaacgaa
     7801 cagatgcgtt cgctattcca ttcggtattc ggtaacgaca gcgtgggaaa acgctcgcga
     7861 taatattttt gtgtgtgagc ggcgcggaaa tgtaacccgg taccaatgcc tctttctttt
     7921 aacgcttcca tcaacgcatt tcgactgata ccacaacatt gttcatcgac acgaataata
     7981 aacagatgcc aggcgtgaac gtgcggccag tcgggaaggc ttaatggctg aaaggggaga
     8041 gctgccagtg cttgctgata ttgctgggca atttcgcgcc gacgggtgtt gaggtgctct
     8101 aatttgacta actgtgtcag ggcaatcgcg gcgttaatat cggtcagatt gtacttatag
     8161 cccggtgtta agacttcagc ctgcggtgca cggccccagg tttgtctgtc ataggcatcg
     8221 acacccagac cgtgaaattt cagcatccgt agctggcggg caaggttttc attatcagtt
     8281 acaatcaggc caccttccgc acaggtaata tttttaatgg catgaaatga aaaaatagcg
     8341 gtaccttttg cgccaatatg tcgccctttg taatacgtac cgacggcatg ggcagcatct
     8401 tcgataactg cgatgccgta acgttcgcca atggcgcgaa tggcgtcaat atctgctggc
     8461 gcaccggcat aatgcaccgg aatgatggct ttagtgcgtg gcgtaatggc tgactcgatc
     8521 gcttcaggcg tgaccatcag cgtatcgcgg tcgacatcca ccattaccgg cgttgcaccc
     8581 aacaaggaaa tcatattgag ggttgaaacc caggtcaggg aaggcgtaat cacttcatcg
     8641 cccttgccaa tttccaacgc cattagcgtg atatgcattc cggcggtggc tgaactgacc
     8701 gcgatggcat gctgatttcc cgtcaactgg caaaaggctt gctcaagcgc ctgattcttc
     8761 ggaccggttg tgatccaacc ggattcgaga acctctttaa ctgcagcgag ttcctccacg
     8821 cccattgctg gtcgcgaaaa aggcaaaaat tctgacattg cttttccttc cgccattgaa
     8881 tacctgtcca cttatatttg ctataaagag tgttgtgtat attttgccat ttggagcgaa
     8941 attttaagga tagaatatta acttaacctt aagaaactaa tattagacgt aaatattgaa
     9001 atttttatat tttttcttat ttaggctttg catttggcaa aattttgagg cattttgccg
     9061 acatcgtagg atttttaata ttacaccaac tgctaattat cgccagaaat gtcgctcaat
     9121 tttacggtaa ttgtctggtt gcgcttgtct ataggtggag tttacgtgtt agctttttgc
     9181 cgctcatcat tgaagtcaaa aaaatatttc atcattttac tggcgctcgc tgcaattgcc
     9241 ggactgggta ctcatgccgc ctggagtagc aatggtttgc cacgtatcga caataaaaca
     9301 ttggccagac tggcacagca gcacccggtt gtcgttttgt ttcgccatgc tgaacgttgc
     9361 gatcgttcaa ccaatcaatg cttgtcagat aaaacaggta ttacggttaa aggtacccag
     9421 gatgcccgtg aactgggcaa cgcttttagc gctgacatcc ctgatttcga tctttattcc
     9481 agtaataccg tccggaccat tcagtcggcg acctggtttt ccgcgggtaa aaagctgacg
     9541 gtagataaac gacttcttca gtgcggtaat gagatttata gtgcaattaa ggacttacaa
     9601 agcaaagcgc ctgataaaaa tatcgttatt ttcacccata atcattgcct gacatatatt
     9661 gctaaaaata agcgtgatgc gacatttaaa cctgattatc tggatggttt agtcatgcat
     9721 gtggaaaaag gcaaagttta tctggatggg gaattcgtta accactaaat tttcaatctg
     9781 acagccagtg atggctgtca tcattgttaa agaagacctt tcaaacgtaa cgtttttcgg
     9841 gtggcaacat ttaaatcata atgcaccaga tcttcaggtt ttacccacgc gtagtcctga
     9901 aactcttcgt ttattttcac ttctcggttg gcagaaacgc agtcaaaaat caggtaaatc
     9961 atataaatct cttccttgcg accatctgca tacgtcttgg tgcgaatatc atcgctgaag
    10021 gtccacggcg tgatttctgt caaaagcagc tgttctccca gttcttcgcg aatttcgcgg
    10081 cgtagtgcct cttcaattcg ttcgccagac tccacgccgc cacccgaaag cgcccattga
    10141 ccggggaaaa cgccgcgatc gtcggccatt ttacacagca aataagcacc atcattttga
    10201 atcaaagggc atacaatagt ccgttgtcgc ataggggtat tccttataaa taaaaagaaa
    10261 ccatgagaat acggtaactc agaaatatgt gaaagtgaca gtaatctctt ttgcaatgat
    10321 aactgcactt attattattt ttgtgtgtat cggtaaagtg tgtttatttt ttcggcgttt
    10381 aattatttaa gaagtattta gttacaataa gtgactagat cccttttttc gctcattgaa
    10441 tcctctgtgc caggcagtac aatcccgcag ctttgggaat tttgattaat tgcttgtggg
    10501 aaaggggata atgaaaaaaa ttgcgctagc aggtctggcc ggaatgctgt tggtttctgc
    10561 atcggtcaat gcaatgagca tcagcggcca ggcgggtaaa gaatacacca atattggtgt
    10621 cggttttggg actgaatcga cgggcctggc tttaagcggt aactggacac ataacgacga
    10681 cgacggtgac gtcgcgggcg tggggctggg gttgaatctg cctctcgggc cgttaatggc
    10741 gaccgttggc ggaaaaggcg tttacaccaa cccgaattac ggcgatgaag gttatgccgc
    10801 agcggtagga ggtggtttgc agtggaaaat tggcaacagc ttccgtttgt ttggcgagta
    10861 ttactactct ccggattcgc tctccagcgg tattcaaagt tatgaggaag cgaatgctgg
    10921 cgcgcgttac accattatgc gtccagtcag tattgaggcg ggttatcgct acctgaatct
    10981 gtcgggtaaa gacggtaacc gcgacaacgc cgtggctgac ggcccgtatg ttggggttaa
    11041 cgccagtttc tgatcccttc cggcgcggca ttctgtcgcg ccattcagtt tcttactacg
    11101 ccttatctcc ctttgcgtta tagtgattcg ataacaaaag caggagataa ccatgttaaa
    11161 agtggaaatg ttatccaccg gggatgaagt gttacacggg caaatcgttg acactaacgc
    11221 tgcctggctg gccgattttt tctttcatca ggggctgcca ttatctcgcc gcaatacggt
    11281 gggggataac cttgatgact tagtcaccat tcttcgcgaa cgtagtcagc acgccgatgt
    11341 gctgatcgtt aacggcgggc tgggaccgac cagcgatgat ttaagcgcac tcgccgctgc
    11401 gacagcaaaa ggtgaaggcc tggtgctgca tgaagcctgg ctcaaagaga tggaacgcta
    11461 tttccacgaa cgtggacgag taatggcacc gagcaaccgt aaacaagcgg agctgcctgc
    11521 cagtgctgaa tttatcaata acccggtagg caccgcctgt ggttttgccg tgcagcttaa
    11581 tcgttgcctg atgttcttta ctcccggcgt accgtcagaa tttaaggtga tggtcgagca
    11641 cgaaatcctg ccgcgcctgc gcgagcgttt ttctttacca cagccgccgg tttgtctgcg
    11701 tttaactacc tttggtcgtt cggaaagcga tctggcacaa agcctggaca ctctacaact
    11761 gccgccgggc gtaacaatgg gctatcgctc ctcaatgccg atcatcgaac tgaaactcac
    11821 cggaccggca agcgagcaac aggcgatgga aaaactgtgg ctggacgtta aacgtgttgc
    11881 cggacagagc gtgattttcg aaggcaccga aggactgccc gcgcagatca gtcgcgaatt
    11941 gcaaaaccgc cagttcagcc tgacgttgag cgagcaattc accggtggtt tattggcttt
    12001 gcaactttct cgcgcaggtg ctccattgct ggcgtgtgaa gtggttcctt cacaggaaga
    12061 aaccctggcg caaactgcgc actggattac agaacggcgg gccaaccatt ttgccgggct
    12121 ggcactggct gtttcgggtt tcgagaacga gcatctcaac tttgcgctag ccacgccaga
    12181 cggcactttc gctctgcgtg tgcgtttcag cactacgcgc tacagcctgg ctatccgtca
    12241 ggaagtgtgc gcaatgatgg cactgaatat gctgcgccgt tggttaaacg gccaggatat
    12301 cgccagtgag catggctgga ttgaggttat tgagtccatg accttatctg tctgattccg
    12361 ccactctgtg ccggtttact gcaccggcac attccccctc cggcaaaact tgtccaaccc
    12421 tccctacggt gagcgtggtg ttggggccag tgtggcacgg gctgcgcgct ggggacgcat
    12481 tgagaattac atggcgcaag tcaacgattc gctttgtctg ttagtgcagg tggaaagtaa
    12541 aacggcactg gataacctgg acgagatcct cgacgtcgaa gggattgatg gcgtgtttat
    12601 tggaccagcg gatctttctg cgtcgttggg ctacccggat aacgccgggc acccggaagt
    12661 gcagcgaatt attgaaacca gcattcggcg gatccgtgct gcgggtaaag cggctggttt
    12721 tctggctgtg gctcctgata tggcgcagca atgcctggcg tggggagcga actttgtcgc
    12781 tgttggcgtt gacacgatgc tctacagcga tgccctggat caacgactgg cgatgtttaa
    12841 atcaggcaaa aatgggccac gcataaaagg tagttattga tatcaaaggc ccatggggat
    12901 cggctgtggg cctgtgttaa ttagtggtta ttcgctgcca gatcggcttc gcttagctgg
    12961 gtggccgcga gcacctggtc acgatcaatg ccctgttcca gcatgcgcaa agcaatttta
    13021 atggcttgtt catgcatacc ttcctgccag ccaatttgat gcccttcctg ccgtagccgt
    13081 tcagcaatag tcattaatct ctccttttgt agtggtgaac gttcggcaat ctcctcaata
    13141 aaacgggtga aacgggaggt atcgccgcat tgcagcagat aattaaacag tgtttgtagc
    13201 tggctgtcat tagtgaagcc tctaaccaaa agcgtggtga tcctgtcgac catgccgatt
    13261 aaatcgcggt cgcgaatatg cttttgaatc agttccagca gagctatacg ccgatgttgc
    13321 atgatctcat cgtcaggtac gatggtgata tccaccaacg gaaaagcttc ggtgtacaac
    13381 tgccgggcaa gttgcggatc gtcaaactca tccagccagt taagtgagta cgggtagggc
    13441 gatgtttcgc catgataaaa cagcaatggc accaccagcg gaactctgtc atagccttta
    13501 tccaggtgac gctgcatggc ggcagtggca tagcgcatta gccgaaaagc catattcttt
    13561 tctgcagagc tttgatgttc aatcacgcag tagatatagc cgtcaccgtc gctggtttcc
    13621 acggaccaca aaacatccga gtagtaagcg cgtaaacttt tttcaataaa actagtgggt
    13681 tccaggcgta aggtttgcag gttgcaaagc ttgcgcagtg gttctggtaa atgtatttcg
    13741 agaaaatccc gtgcggtttc gggtgtgaac ataaaggttt taaacaccgc atcatgcggg
    13801 gaggatgttg ttgattcggt catggttgtc cgtcacctga tatttatgat gacgcgacat
    13861 tacgtctatc ccgggccttt gacatctgtc agtttcagcc tttgcgagac attttcagag
    13921 gaattttcaa agcgttgcaa cgcatacatg gtgcgtggaa tcgcgctcgc aaaaatgccc
    13981 gataagcaat gtgcttaccg ggcatcaaaa ggtcggttaa gccagcgcct gggccagtag
    14041 cgtaatcgga tgttcgcagc gaaggctcgt ggacatctca atctgccatt tgcaggtttc
    14101 gcagtcggtg atgaccagat ccgcgccgct ctcttctatc tggcggaaca gtggcgcacc
    14161 gatggcttgt gaggtggtgt agttctcttt tttgaaaccg taagtacccg caataccgca
    14221 gcactgggaa tccagcaccg ttaactcaag ccccgggatt ttacgcaaca gctccagggt
    14281 gtagagcgtc cagcccattt tttccatatg gcacggagtg tgataaacca ctttcagcgg
    14341 cagcggtttc agcggtaacg ttttgccttc gtccagcttg cgccacagcc agcgggttgc
    14401 cagttcgata tgatcgcgca agcctttgtt gtcgacattc agcacttccg ggtattcgtc
    14461 gcgcagggca aatgtacagg ttgaggaggt ggcaatcacc ggaatgcctt ttactcccac
    14521 agcttcgcgg atcgactcta cattcgtaat tgcctgtttg cgtgctttat cggtaaagcc
    14581 gttggcgatt agcggtacgc cgcagcattt ttctttgctg agcagttgta caccggtacc
    14641 cattgcgttg agcactttaa ttaaatcttt acctaactgc ggatggttgt agttaacgaa
    14701 gcagccgtga aagaaagcga cctggtcttt atattgtgct tgctgagccg ctatgctgcg
    14761 ataccagcga cgaaacgtac cgaaggagta tttcggtagc gtgcggcgat gatcgatttt
    14821 taacgccgca tcaagcagct gccgcaccgg tttcagcgag gtagcggtgt agacgattgg
    14881 tgcgaacggc gtggaaacgc tacccatcag gtcggtatga ctcaacacaa aattacgcag
    14941 cgacgggcgc gtggtgtcat attttgcccg cgcgcgctgg ataatatcgc caatcttcac
    15001 atcggacggg caggcgactt cacaacgttt gcagttgatg caatatttca gcgcctcgtc
    15061 atacagtgcg ccatctttca aacgcagacg ctcgccatcc ggcccggctt gttttggccc
    15121 tggataaccg ggattcaccc ggctcaccgg gcaggcggtg gtgcagacgg tgcacttaat
    15181 gcagttttcg aagctggtgt cgttcattgt tggcctcctg cgcgttgggc aatctgttga
    15241 gcggcatgta aagcactgac ggcacaaaca ccgccgccgc atccctgggc gatgggatca
    15301 aatccgccca gcaccgaacc gatggcaaac aggttctcaa tggtttgccc tgcctgcgac
    15361 gggcgtagcg tctgatcagt ggttacaccg aactgctgcc acggttgcgg cgcaaaaaaa
    15421 tcgcccttat accattcacc ccgcgtggcg gtttgtagca catcaaggcc gagaatcggc
    15481 tcgcgaatgc cgttacgttc cgctaccagt ccgccactaa agaaactgcc gctggcgaga
    15541 accgcgaaac gtggacgtag cggaatatcg gcgtgattgc gggtccagat ttcattcact
    15601 acgccatttt tacaggtcac ttttttcact tcatcgcccg gcatccacac gccaccctgg
    15661 cgcacaaact ggcgctgtaa ctggttttgc agacgaatgc ccagcacgga aggcggcagc
    15721 gttggcaaaa gcatcagtga acaaggtagt ttttcattca accaacgcca cagtttgtca
    15781 tcggccaggc cgaagcaggc gggcatcagg atcatttcgc a
//
LOCUS       DAMZET010000094        15771 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.094, whole genome shotgun sequence.
ACCESSION   DAMZET010000094 DAMZET010000000
VERSION     DAMZET010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.094"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            279..386
                     /locus_tag="POB34_003035"
     CDS             279..386
                     /locus_tag="POB34_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDI5919938.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(473..2152)
                     /gene="bcsG"
                     /locus_tag="POB34_003036"
     CDS             complement(473..2152)
                     /gene="bcsG"
                     /locus_tag="POB34_003036"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HDI5919939.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2149..2340)
                     /gene="bcsF"
                     /locus_tag="POB34_003037"
     CDS             complement(2149..2340)
                     /gene="bcsF"
                     /locus_tag="POB34_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HDI5919940.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2337..3908)
                     /gene="bcsE"
                     /locus_tag="POB34_003038"
     CDS             complement(2337..3908)
                     /gene="bcsE"
                     /locus_tag="POB34_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HDI5919941.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            4181..4369
                     /locus_tag="POB34_003039"
     CDS             4181..4369
                     /locus_tag="POB34_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HDI5919942.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4381..5133
                     /gene="bcsQ"
                     /locus_tag="POB34_003040"
     CDS             4381..5133
                     /gene="bcsQ"
                     /locus_tag="POB34_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HDI5919943.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPGNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLHYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            5130..7748
                     /gene="bcsA"
                     /locus_tag="POB34_003041"
     CDS             5130..7748
                     /gene="bcsA"
                     /locus_tag="POB34_003041"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HDI5919944.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            7759..10098
                     /gene="bcsB"
                     /locus_tag="POB34_003042"
     CDS             7759..10098
                     /gene="bcsB"
                     /locus_tag="POB34_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HDI5919945.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERVWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            10105..11211
                     /gene="bcsZ"
                     /locus_tag="POB34_003043"
     CDS             10105..11211
                     /gene="bcsZ"
                     /locus_tag="POB34_003043"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HDI5919946.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            11193..14666
                     /gene="bcsC"
                     /locus_tag="POB34_003044"
     CDS             11193..14666
                     /gene="bcsC"
                     /locus_tag="POB34_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HDI5919947.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPANADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQRTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLHDC
                     SGNRSQSDSGASVAVGWRNDTWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRITSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            14748..>15771
                     /locus_tag="POB34_003045"
     CDS             14748..>15771
                     /locus_tag="POB34_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HDI5919948.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQV"
BASE COUNT         3707 a         4080 c         4410 g         3574 t
ORIGIN      
        1 cggttgcaag cggataatat tctgcggaat gcgcagcaca gagtgagtaa gtgatggatt
       61 tcggaacaga aaaacagtcg tagaatcagc ggcaagccgg aaaggttccg gtaaggcgca
      121 atgttgcggg ggctttatcc ctggtggcat tggttgctgg agagagaaaa cccccgcacg
      181 ttgcaggtat gcacctgaca acataacggg ggctaatctt gaacccaaac aactcaagaa
      241 tagccgcgaa acgttgtcat tacaacacag gcggctatat gacgctcgca gagctgggca
      301 tggccttctg gcatgattta gcggctccgg tcattgctgg cattcttgcc agtatgatcg
      361 tgaactggct gaataagcgg aagtaacgtg tcatgcgggc gtcaggctgc cgtaatggca
      421 atttgcgccc ggatcaggcc gcagaggtta aactctgcgg cctttttcgt gcttactgcg
      481 ggtaaggcac ccagtcgccg ccgttcaggc gaacgtacgg tttatcctgg tattgaataa
      541 ctactgcatt tgagttctcg gagaccggtg ctgtttgtgg caacccactg gtgagttttt
      601 tccagtcaac attgtcttcg gtgaaaatct tgccatcaag aacgcgaacc accagatcgg
      661 agatagccag gaagctgctc ggttggtcaa tgacaatcgg tgccccctga tgtggtgcct
      721 tcatgccgaa gaatttcacc ccaacgggga cgtctgtgat agacgggcta gggatatcac
      781 gtaggccaga tacctgcatt ctgtcgccct tcagcgcgcc gccgtgttcc ggcaccacga
      841 ccaccatcac tttacgaccc gatttctcca gttcagtaaa gaaggcgtcc agttcatcaa
      901 agaatttctg cgcccgcgct ttgtaatccg ctgttttgct gacccccgga taatggttgc
      961 cgtcatgcag tggaagcgtg ttgtagaacg tggcactacg gctattttta tctttttcgg
     1021 taacgtccag ccagcggtta agcacggcgg tatcgtcata aaccggcgaa ccatcaaagc
     1081 ccagcaaaat aaccggcaga tttgtttgat ccatcaattc agtctgcatg ccgccatttt
     1141 cgcgaacttc tttcaaaaaa ccgccgaact ggccgttatg ccccatcatc aggtgctggg
     1201 taaagcccag tttcgaaagg ttatcaaaca gatagcagtc gttatttgcc ggttgataca
     1261 gattagtgtg cgaagtctgc ccgcagctgg cgcgcagtaa acggatcgcc gccgggccac
     1321 tgtaggaggt ggcggagtta aagttcttga actcaatatc gaaatgcgac cacagtggat
     1381 gcgacatcaa cccggcggct tctatatccg accaggaaag cgaacagatg ttaatcacca
     1441 gtagttcaaa tggctgagca tcagcgggca gcgaagacgg gaaggtcgat ttacgtttcg
     1501 cctccgcgtt atagaaatta ttcagccagg cgttaaggtt cgccgttgtt ggcggtgcag
     1561 tttgtgcggg catatcaccc actaccggtg cgccacccgt cgccgcaacg gttgccgctg
     1621 cgttaccacc cgtcgttgtt acagtggtcg tcggttgtcc ggctggccac aaggagaaac
     1681 ttggtcccgc cagggtaagt acgttcagcc atagcagtat ggcaaccaca aaaacggtaa
     1741 tgcgaatcca ttgtgacagg aataaccagg ccactaataa aacaaaaatg gccccaatca
     1801 tctgccagtt aataaagcgt gtgacaaggt cgattaaata atcggtactg aaccccgcca
     1861 cctgcgaacc ctggctcatt atgctttccg ggccaggcaa ccaggtgtca tgccagaaca
     1921 aagcaaagcc gatcggcagg gcaatccagt ggcgcaagcg atgtaggctg tagcgcggaa
     1981 tgggcatcag cagaaacgcg gcaaacacca aattgaggag cggatggaag ttaagatatc
     2041 ccgcccacaa caggccgaac ttaaccagaa aatagaagtt ccagccggaa aggccgcgcc
     2101 agtattgcca gagggaagaa ggcatggcgg tattttgcgt aaattgagtc attttttggt
     2161 tgccctggct ttttccgtgc ggcgtaacgt cccggccggt ttaacataac gaggtttagc
     2221 aaagaacaaa cgtaaggtgt cgcgaatgcg tcgcaaagag tgccgcgcca gatagcccag
     2281 cgggaaaaat atcagtgcgc aaacgacaat aatttcaatg atatcgctga tggtcatcat
     2341 gatgagcgct ccacagcatc atctaacagt cgcatgggtt ctggtattcg tcgccagtgg
     2401 cgaccatcgt gctcggcatt gatgaccggt ttagaacttt gcgttaaagg cagcggcatg
     2461 ccccattgtt ctggggcaag caagcgcatc tgcaccagct cggcactgat ttgatcatct
     2521 tcaaaccaga ccatacggtt tgagaaaatg tcgccagtag gcaatgggaa aatatgattc
     2581 aacgcggtat ccagatcgtt aatccgacag aatgagagaa acagcaccag ccgattaccg
     2641 ccaatggtca tgatatcgcc ggtacggtta gggcgacaca gcgtcagggc ttgttcaacg
     2701 cggatacccg gtaccggacg tagggcaacc agaacgcctt taccgtgggc aggcaagaga
     2761 gggttattca tcatgttgtt gacggcatta cagaacacat cccacttctg gaaaccacgc
     2821 agtttgagcg gctgggtcat tgacagcaag gtagtgatat cttccggcac atagcgacta
     2881 aacttctgcc cttgcacgct ttcgatcatc gtcagacaac gggagagtgg cgcattccac
     2941 ggaataacca tatttgcacc gcaggccaat aacaaacgtt catcggtggc gcgcaggcta
     3001 gcggtatttt cccgcacgag gattttcatc gcactaccgc gctggcgacg cagggtatga
     3061 atgctgcggg ccagtggctc gatttgcgca ttttgctgta aagaaaagac caccgtcgcc
     3121 gcctgagcgg tacgggcttc attgaacagg acttcgttat tgttgaacag ttgccagtgt
     3181 tccgatagcg gcggcgcacc ttccagtaca gcaacattac tcagaatgcg tttttcgtcg
     3241 ctgcgtggtt ggatctccgc ctcttcgctt tgaactaatg tccagatacc attttgttgc
     3301 tgaacgctaa gctgctgacg ggcgctgacc cctttttcgt tgcaccagaa ggcaatatcc
     3361 agcaaatgtt ggtcaccctg aaaacgcaaa ctggcaagac caaaaagtga acggtattcc
     3421 tcaagcaaca atgaaaattg tttatcgtta ttatttccgg gattaattac caaaagcgaa
     3481 caatggttta acctgctcca tttattcatt ttatccaacc atgagcgaag ccgctcggca
     3541 ggaatgtttt gccatgcgtt atttgcgcaa acaagaataa aaaggtaatt atggggatca
     3601 atagaacact gcaaatcacg ggtcaaatag tatagacctt tttcatgatt tagcattgaa
     3661 aataatttta ttttttccgg cccttgagaa tcatctaatt gaaagatttt cgccggatcg
     3721 ctgtccatgc taatgaccgc gacgtgtgcg gtttcagcct gggatgcaat tgtttgattc
     3781 gccagactga tagcatcttc atggcgatcg acgttaaacc accagacgcc gcctgctggc
     3841 atatgtcgca gctcatccca taatgatgag ataccgatag agaatacagg gtccacaatg
     3901 tccctcatgt caccattttg tctgattatc agtttactag cgaaagctaa gaaataaacc
     3961 taccattgaa attaaaactt atcagattta gcatgtgaat caatttagaa ggtcacaaga
     4021 cgtgtgattt tagtcttttc gtttttaact ttttctgttg cgtaacggaa agtcaaaaag
     4081 tgagcaaatt cccgtctcgc cgtaaacaat aaaccggcga aatgctcacg gttagaattg
     4141 tctcatgaac ggtacggtta tttcataggg atcaagcaaa atgaataaca atgaaccaga
     4201 tactctgcct gatcccgcga taggctatat cttccagaat gatattgtgg cgttaaagca
     4261 ggcattttca ctgcctgata ttgattatgc cgatatttcc caacgcgaac agttggccgc
     4321 ggcattaaaa cgctggccgt tgctggcaga gtttgcgcaa caaaagtagg ggattggtga
     4381 atggccgtac tgggattgca gggggtgcgg ggaggcgtgg ggacaacaac catcaccgcc
     4441 gcattagcct ggtcattaca aatgttggga gaaaatgtcc tggtggtcga tgcctgcccg
     4501 ggcaacttgt tgcgcctgtc atttaatgtt gattttaccc accgtcaggg ctgggccaga
     4561 gcgatgctgg atggccagga ctggcgtgac gctgggttgc actacacctc gcagctcgat
     4621 ttgctgcctt ttggtcagtt atccattgaa gaacaagaaa atccacagca ctggcaaacc
     4681 cggctgagcg atatttgctc cggcttacag caactaaaag ccagcgggcg ttaccagtgg
     4741 attttaatcg acttaccgcg tgatgcctcg cagataaccc accagctgct gagtttgtgc
     4801 gatcactcgc tggcaatcgt caatgtggat gccaactgcc atatccgact gcatcagcaa
     4861 gcgctgccgg atggcgcaca tattttgatt aatgacttcc gtattggcag tcaggttcag
     4921 gacgatattt accagctttg gttgcaaagc cagcgccgat tactgccgat gctcattcat
     4981 cgtgatgaag cgatggctga atgcctggcg gctaagcaac cagtaggtga atatcgcagt
     5041 gatgcgctgg cggctgaaga gatactgacg ctggcgaact ggtgcctgtt gaactactcc
     5101 gggctgaaaa cgccagtcgg gagtgcatca tgagtatcct gacccggtgg ttgcttatcc
     5161 cgccggtcaa cgcgcggctt atcgggcgtt atcgcgatta tcgtcgtcac ggtgcgtcgg
     5221 ctttcagcgc gacgctcggc tgtttctgga tgatcctggc ctggattttt attccgctgg
     5281 agcacccgcg ctggcagcgt attcgcgcag aacataaaaa cctgtatccg catatcaacg
     5341 cctcgcgtcc gcgtccgctg gacccggtcc gttatctcat tcaaacatgc tggttactga
     5401 tcggtacatc gcgcaaagaa acgccgaaac cgcgcaggcg ggcattttca ggtctgcaga
     5461 atattcgtgg acgttaccat caatggatga acgagctgcc tgagcgcgtt agccataaaa
     5521 cacagcatct tgatgagaaa aaagagctcg gtcatttgag tgccggggcg cggcggttga
     5581 tcctcggtat catcgtcacc ttctcgctga ttctggcgtt aatctgcgtt actcaaccgt
     5641 ttaacccgct ggcgcagttt atcttcctga tgctgctgtg gggtgtagcg ctgatcgtac
     5701 ggcggatgcc ggggcgcttc tcggcgctaa tgttgattgt gctgtcgctg accgtttctt
     5761 gccgttatat ctggtggcgt tacacctcta cgctgaactg ggacgatccg gtcagcctgg
     5821 tgtgcgggct tattctgctc ttcgctgaaa cgtacgcgtg gattgtgctg gtgctcggct
     5881 acttccaggt agtatggccg ctgaatcgtc agccggtgcc attgccgaaa gatatgtcgc
     5941 tgtggccgtc ggtggatatc tttgtcccga cttacaacga agatctcaac gtggtgaaaa
     6001 atactattta cgcctcgctg ggtatcgact ggccgaaaga caagctgaac atctggatcc
     6061 tcgatgacgg cggtagggaa gagtttcgcc agtttgcgca aaacgtgggg gtgaaatata
     6121 tcgcccgtac cactcatgaa catgcgaaag cgggcaacat caacaatgcg ctgaaatatg
     6181 ccaaaggcga gttcgtgtcg attttcgact gcgaccacgt accaacgcga tcgttcctgc
     6241 aaatgaccat gggctggttc ctgaaagaga aacagctggc gatgatgcag actccacacc
     6301 acttcttctc gccggacccg tttgaacgca acctggggcg tttccgtaaa acaccgaacg
     6361 aaggcacgct gttctatggt ctggtgcagg atggcaacga tatgtgggac gccactttct
     6421 tctgcggttc ctgtgcagta attcgtcgca aaccattgga tgaaattggc ggtattgctg
     6481 tcgaaacggt aaccgaagat gcgcacacgt ctctgcgcct gcaccgtcgt ggctacacct
     6541 ctgcatatat gcgtattccg caggcggcgg ggctggcgac cgaaagtctg tcggcgcata
     6601 tcggtcagcg tattcgctgg gcacgcggga tggtgcaaat cttccgtctc gataacccgc
     6661 ttaccggtaa agggctgaaa ttcgcccagc ggctgtgcta cgtcaacgcc atgttccact
     6721 tcttgtcggg cattccacga ctgatcttcc tgactgcgcc gctggcgttc ctgctgcttc
     6781 atgcctacat catctatgcg ccagcgttga tgatcgccct gttcgtgctg ccgcatatga
     6841 tccatgccag cctgaccaac tcgaagatcc agggcaaata tcgccactct ttctggagtg
     6901 aaatctacga aacggtgctg gcgtggtata tcgcaccacc gacgctggtg gcgctgatta
     6961 acccgcacaa aggcaaattt aacgtcaccg ccaaaggtgg actggtggaa gaagagtacg
     7021 tcgactgggt gatctcgcgg ccctacatct tccttgttct actcaacctg gtgggcgttg
     7081 cggtaggcat ctggcgctac ttctatggcc cgccaaccga gatgctcacc gtggtcgtca
     7141 gtatggtgtg ggtgttctac aacctgattg ttcttggcgg cgcagttgcg gtatcggtag
     7201 aaagcaaaca ggtacgccga tcgcaccgcg tggagatgac gatgcccgcg gcaattgccc
     7261 gcgaagatgg tcacctcttc tcgtgtaccg ttcaggattt ctccgacggt ggtttgggga
     7321 tcaagatcaa cggtcaggcg cagattctgg aagggcagaa agtgaatctg ttgcttaaac
     7381 gcggtcagca ggaatacgtc ttcccgaccc aggtggcgcg cgtgatgggt aatgaagttg
     7441 ggctgaaatt aatgccgctc accacccagc aacatatcga ttttgtgcag tgtacgtttg
     7501 cccgtgcgga tacatgggcg ctctggcagg acagctaccc ggaagataag ccgctggaaa
     7561 gtctgctgga tattctgaag ctcggcttcc gtggctaccg ccatctggcg gagtttgcgc
     7621 cttcttcggt gaagggcata ttccgtgtgc tgacttctct ggtttcctgg gttgtatcgt
     7681 ttattccgcg ccgcccggag cggagcgaaa cggcacaacc atcggatcag gctttggctc
     7741 aacaatgatg ataacgcgat gaaaagaaaa ctattctgga tttgtgcagt ggctatgggg
     7801 atgagtgcgt tcccctcttt catgacgcag gcgacgccag caacgcaacc actgatcaat
     7861 gctgagccag ctgtagccgc ccagacggaa caaaatccgc aggtggggca agtgatgccg
     7921 ggcgtgcagg gcgctgatgc gccagtcgtg gcgcagaacg gtccttcgcg tgatgtgaag
     7981 ctgacctttg cgcaaattgc gccgccgccg ggcagcatgg tgctacgtgg cattaacccg
     8041 aacggcagca ttgagtttgg tatgcgcagc gatgaagtgg tgacgaaggc gatgctcaac
     8101 ctcgaataca cgccatcgcc atcgttactg cctgtccagt cgcagttaaa ggtttatctc
     8161 aatgatgagc tgatgggcgt gctgccagtg accaaagaac agttgggtaa aaaaacgctg
     8221 gcgcaaatgc ccattaaccc actgtttatt accgacttca accgtgtgcg gctggagttt
     8281 gtcggccatt atcaggacgt gtgcgaaaac ccggccagca ccacgctttg gctggatgtt
     8341 gggcggagca gtggactgga tctgacctat cagaccctga atgtgaagaa tgacctgtca
     8401 cacttcccgg tgccattctt tgacccgcgc gataaccgta ctaacacctt gccgatggtc
     8461 tttgcgggtg cgccggatgt tgggctgcaa caagcctctg ccattgtcgc ctcgtggttt
     8521 ggttcgcgtt ctggctggcg tgggcagaac ttcccggtgc tctataacca actgccggat
     8581 cgtaatgcca ttgtctttgc aaccaacgac aaacggccgg acttcctgcg cgatcatccg
     8641 gcggtaaaag cccctgtgat tgagatgatt aaccatccgc agaatcctta cgtcaaactg
     8701 ctggtggtgt ttggtcgtga cgacaaagac ctgttgcagg cagcgaaagg tatcgctcag
     8761 ggtaacattc tgttccgtgg tgaaagcgtg gtagtgaatg aagtgaaacc gctgctaccg
     8821 cgtaagccgt acgatgcgcc gaactgggta cgtaccgatc gtccggtcac atttggcgaa
     8881 ctgaaaacct atgaagaaca gttacaatcc agcggtcttg agccagcagc gattaacgtt
     8941 tcgctaaacc tgccgccgga tctctacctg atgcgcagta ccggcattga tatggatatt
     9001 aattaccgct acaccatgcc gccggtgaaa gacagttcgc ggatggatat cagcctgaat
     9061 aaccagttcc tgcaatcctt caacctgagc agcaaacagg aggcgaaccg cctgctgctg
     9121 cggattccgg tattacaagg tttgctggat ggcaaaacag atgtctctat tccggcgctg
     9181 aaactgggcg cgaccaacca gctgcgcttc gactttgagt atatgaaccc gatgccgggc
     9241 ggttcggtgg ataactgtat taccttccag ccggtgcaga atcatgtggt gattggtgac
     9301 gactccacca tcgacttctc gaagtattac cacttcatcc cgatgccgga tctacgcgcc
     9361 tttgctaacg cgggcttccc attcagccgg atggcggatc tgtcgcaaac catcaccgtg
     9421 atgccgaaag cgcctaacga agcacagatg gaaacgttgc tgaatactgt tggttttatc
     9481 ggcgcacaga cgggcttccc ggcgattaat ctgacggtga ccgatgatgg cagcaccatt
     9541 cagggcaaag atgccgacat catgatcatc ggtggtatcc cggacaaact gaaagacgat
     9601 aagcagatcg acctattggt gcaggcgacc gaaagctggg tgaaaacacc gatgcgccag
     9661 accccgttcc ccggcattgt gccggacgag agcgatcgcg cggcagaaac ccggtcaacg
     9721 ctgacctctt ccggtgcgat ggcggcggtg attggcttcc agtcgccgta taacgaccag
     9781 cgcagcgtga ttgcgctgtt ggcagatagc ccacgcggtt atgaaatgct taacgatgcg
     9841 gtgaacgata gcggcaaacg cgccaccatg ttcggttcgg tcgcggtgat ccgcgagtcc
     9901 ggtatcaaca gcctacgtgt tggcgacgtt tattacgtag gtcatctgcc gtggttcgag
     9961 cgcgtgtggt atgcgctggc aaaccatccg attctgctgg cggtgctggc ggctatcagt
    10021 gtgatattgc tggcatgggt actgtggcgt ctgctgcgaa ttattagtcg tcgtcgtctt
    10081 aacccggata acgagtaatt gaagatgaat gtgttgcgta gtggaatcgt gacgatgctg
    10141 ctgctggctg cctttagtgt tcaggcagcc tgtacctggc ctgcctggga gcagtttaaa
    10201 aaggattaca tcagtcagga agggcgcgtc atcgacccca gcgacgcgcg caaaatcacc
    10261 acctccgaag ggcaaagtta cggtatgttc tttgccctgg cggctaacga ccgtgtagct
    10321 ttcgataata ttctcgactg gacgcagaac aatctcgctc agggctcttt aaaagaacgt
    10381 ttgcccgcct ggctgtgggg caagaaagag aacagtaagt gggaagtgct ggacagcaat
    10441 tcggcctccg atggtgatgt ctggatggcc tggtcgttgc tggaggcggg gcgtttgtgg
    10501 aaagagcagc gttataccga catcggcagc gcgttgctaa aacgtatcgc gcgggaggaa
    10561 gtggtgacgg tgcctgggct gggttccatg ttgttaccgg gcaaagtggg ttttgctgag
    10621 gataacagct ggcgttttaa ccccagctac ctgccgccga cgctggcgca gtatttcacc
    10681 cgctttggcg cgccgtggac cacgctgcgc gaaaccaatc aacgtttatt gctggaaacc
    10741 gccccgaaag gtttttcgcc agactgggtg cgctatgaga aagacaaagg ctggcagcta
    10801 aaagccgaaa aaacattgat cagcagctac gacgctatcc gcgtttacat gtgggtaggc
    10861 atgatgcctg acagcgatcc gcaaaaagcg cggatgctca accggtttaa accgatggcg
    10921 acattcactg agaaaaacgg ttatccgccg gaaaaagtgg atgtggctac ggggaaagcg
    10981 cagggtaaag gaccggtcgg tttttctgcc gccatgctgc cctttttaca aaaccgcgat
    11041 gcgcaggccg ttcagcgcca gcgcgtggcc gataactttc ccggcagcga tgcctattac
    11101 aactatgtgc tgaccctgtt tggacaaggc tgggatcaac accgtttccg cttctcgaca
    11161 aaaggtgagt tattacctga ctggggccag gaatgcgcaa attcacacta aacatattca
    11221 cgctttccct cggtctggcc gtcatgccga tggtcgaggc agcaccaacc gctcagcaac
    11281 agttgctgga gcaagttcgg ttaggcgaag cgacccatcg tgaagatctg gtgcaacagt
    11341 cgttatatcg gctggaactt attgatccga ataacccgga cgtcgttgcc gcccgtttcc
    11401 gttctttgtt acgtcagggc gatattgatg gcgcgcaaaa acagctcgat cggctgtcgc
    11461 agttagcgcc gagttcaaat gcgtataaat cgtcgcggac tacgatgcta ctttccacgc
    11521 cggatggtcg tcaggcactg caacaggcac gattgcaggc gacgaccggt catgcagaag
    11581 aagctgtggc gagttacaac aaactgttca acggtgcgcc gccggaaggt gacattgccg
    11641 tcgagtactg gagtacggtg gcgaaaattc cggctcgccg tggcgaagcg attaatcagc
    11701 taaaacgcat taatgcggat acgccgggca atacgggcct gcaaaacaat ctggcgctat
    11761 tgctgtttag tagcgatcgg cgtgacgaag gttttgccgt cctggaacag atggcaaaat
    11821 cgaacgccgg gcgcgaaggg gcctctaaaa tctggtacgg gcagattaaa gacatgcccg
    11881 tcagtgatgc cagtgtgtcg gcgctgaaaa aatatctctc gatctttagt gatggcgata
    11941 gcgtggcggc tgcgcaatcg caactggcag aacagcaaaa acagctggcc gatcctgctt
    12001 tccgcgctcg tgcgcaaggt ttagcggcgg tggactctgg tatggcgggt aaagccattc
    12061 ccgaactaca acaggcggtg cgggcgaacc cgaaagacag tgaagctctg ggggcgctgg
    12121 gccaggcgta ttctcagaaa ggcgatcgcg ccaatgcagt ggcgaatctg gaaaaagccc
    12181 tcgcactgga cccgcacagc agcaacaacg acaaatggaa cagtctgctg aaagtaaacc
    12241 gctactggct ggcgatccag cagggcgatg ctgcgctgaa agccaataat cctgaccggg
    12301 cagaacgcct gttccagcag gcgcgtaatg tcgataacac cgacagttat gcagtgctgg
    12361 ggctgggcga tgtggcgatg gcgcgaaaag attatcccgc cgccgaacgt tattatcagc
    12421 agaccttgcg tatggacagc ggcaacacta acgccgtgcg cgggctggca aatatttacc
    12481 gccagcaatc gccagaaaaa gctgaagcgt ttatcgcctc gctctctgcc agtcagcggc
    12541 gtagcattga tgatatcgaa cgcagcctgc aaaacgaccg tctggcacag caggcagagg
    12601 cactggaaaa ccagggcaaa tgggcgcagg cggcagcact tcagcggcaa cgactggcgc
    12661 tggaccccgg cagcgtatgg attacttacc gactttcgca ggatctctgg caggccggac
    12721 aacgcagcca ggccgatacg ttaatgcgca atctggcgca gcagaagccg aacgacccgg
    12781 agcaggttta cgcttacggg ctgtacctct ctggtcatga ccaggacaga gcggcgctgg
    12841 cgcatatcaa cagcctgccg cgcgcgcagt ggaacagcaa tattcaggag ctggttaatc
    12901 gactgcaaag cgatcaggtg ctggaaaccg ctaatcgcct gcgagaaagc ggcaaagaag
    12961 ctgaagcgga agcgatgctg cgccagcaac caccttccac gcgtattgac ctcacgctgg
    13021 ctgactgggc gcaacaacga cgtgattaca ccgccgcccg cgctgcatat cagaatgtcc
    13081 tgacgcggga gccagctaac gccgacgcca ttcttggtct gacggaagtg gatattgctg
    13141 ccggtgacaa agcggcggca cgtagccagc tggcgaaact gcccgctacc gataacgcct
    13201 cgctgaacac acagcggcgc gtggcgctgg cacaggcgca gcttggcgat accgcagcag
    13261 cgcagcggac gtttaataag ttgatcccgc aggcaaaatc tcagccaccg tcgatggaaa
    13321 gcgcgatggt gctgcgtgat ggtgcgaagt ttgaagcgca ggcgggcgat ccaaagcagg
    13381 cgctggaaac ctacaaagac gccatggtcg catccggtgt gactacgacg cgtccgcagg
    13441 ataacgacac ctttacccga ctgacccgta acgacgagaa agatgactgg ctgaaacgcg
    13501 gcgtgcgcag cgatgcggcg gacctctatc gccagcagga tcttaacgtc accctcgagc
    13561 atgattactg ggggtcgagc ggcaccggtg gttactccga tctgaaagcg cacactacca
    13621 tgttgcaggt ggatgcgccg tattctgacg ggcggatgtt ctttcgcagt gatttcgtca
    13681 atatgaacgt cggcagtttc tccactaatg ccgatggcaa atgggatgac aactggggta
    13741 cctgtacgtt gcacgactgt agtggcaacc gcagccagtc ggattccggt gccagcgtgg
    13801 cggttggctg gcgcaacgac acctggagct gggatatcgg caccacgccg atgggcttca
    13861 acgtggtgga tgtggtcggc ggcatcagtt acagcgatga tatcgggccg ctgggttaca
    13921 ccgttaacgc ccaccgtcgg cccatctcca gttctttgct tgcctttggc gggcaaaaag
    13981 actccccgag caataccggg aaaaaatggg gtggcgtacg tgccgacggt gtggggctaa
    14041 gtctgagcta cgataaaggt gaagcaaacg gcgtctgggc atcgcttagt ggcgaccagt
    14101 taaccggtaa aaatgtcgaa gataactggc gcgtgcgctg gatgacgggc tattactata
    14161 aggtcattaa ccagaacaat cgccgcgtca caatcggcct gaacaacatg atctggcatt
    14221 acgacaaaga cctgagtggc tactcactcg gtcagggcgg ttactacagt ccgcaggaat
    14281 acctgtcgtt tgccataccg gtgatgtggc gggagcgcac ggaaaactgg tcgtgggagc
    14341 tgggggcgtc tggctcgtgg tcgcattcac gcaccaaaac catgccgcgt tatccgctga
    14401 tgaatctgat cccgaccgac tggcaggaag aagctgcgcg gcaatccaac gatggcggca
    14461 gcagtcaggg cttcggctac acggcgcggg cattacttga acgacgtatt acttccaact
    14521 ggtttgttgg cacggcaatt gatatccagc aggcgaaaga ttacgcaccc agccatttcc
    14581 tgctctacgt acgttattcc gccgccggat ggcagggtga catggattta ccgccgcagc
    14641 cgctgatacc ttacgccgac tggtaagttt tcagatagcg cctctcttaa tgccgctgcg
    14701 atcgggtata ctcgggcggc aatctgggat ttccgggggg agacaatttg cgcgtaagtc
    14761 gctcgttaac aatcaagcag atggcaatgg tggcagccgt tgtcctggtg ttcgttttta
    14821 ttttttgcac cgttttgctg ttccatctgg tccagcagaa tcgctataac acggctacgc
    14881 aactggaaag cattgctcgc tctgtccgcg aacccttatc ttcagctatt ttgaaaggcg
    14941 atattcccga agcggaagct attcttgcca gcattaaacc ggcaggcgtg gtcagccgtg
    15001 ccgatgtagt gctgcctaac cagttccagg cgctgcgtaa aagttttatt ccagagcgtc
    15061 cggtgccggt aatggttact cgcctgtttg agctaccggt tcaaatctcg ctgggcgtct
    15121 actcgctcga acgtccggca aatccgcagc caattgccta tctggtgcta caggcggatt
    15181 ccttccgtat gtataagttc gtgatgagca ccctctcaac gttagtgacc atttacttac
    15241 ttttgtcgtt aatattgacg gtggcgatta gctggtgcat taaccgcctg attttgcatc
    15301 cgttacgcaa tattgctcgc gaacttaacg ccatcccagc ccaggagctt gttggtcacc
    15361 aactggcatt accgcgtctg catcaggacg atgaaatcgg tatgttggtg cgcagttaca
    15421 acctcaacca gcaattgctg cagcgccatt atgaagaaca gaacgaaaat gcgatgcgct
    15481 tcccggtgtc ggatttgccg aacaaagcct tgctgatgga gatgctggag caggttgtcg
    15541 cgcgtaaaca aaccaccgcg ctgatgatca tcacctgtga aaccctgcgt gatactgcgg
    15601 gcgtgctgaa agaggcgcaa cgagaaattc tgctgctgac gctggtggaa aaactcaaat
    15661 cggtactgtc gccacgtatg atcctcgcgc agattagcgg ttatgacttt gctgtcattg
    15721 ccaacggtgt acaggaaccg tggcacgcaa tcaccttagg tcagcaagtg c
//
LOCUS       DAMZET010000095        15681 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.095, whole genome shotgun sequence.
ACCESSION   DAMZET010000095 DAMZET010000000
VERSION     DAMZET010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.095"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(1..526)
                     /locus_tag="POB34_003046"
                     /pseudo
     CDS             complement(1..526)
                     /locus_tag="POB34_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(787..1236)
                     /locus_tag="POB34_003047"
     CDS             complement(787..1236)
                     /locus_tag="POB34_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919949.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEISDLYAGKIIDLLQMKYFRKEKYNNIRWGVSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
     gene            complement(1248..4221)
                     /locus_tag="POB34_003048"
                     /pseudo
     CDS             complement(1248..4221)
                     /locus_tag="POB34_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            4282..4764
                     /locus_tag="POB34_003049"
     CDS             4282..4764
                     /locus_tag="POB34_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HDI5919950.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            4808..5721
                     /locus_tag="POB34_003050"
                     /pseudo
     CDS             4808..5721
                     /locus_tag="POB34_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5731..6210
                     /locus_tag="POB34_003051"
     CDS             5731..6210
                     /locus_tag="POB34_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919951.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLILCFGFTSFSFLNIHVILLTIIKSFLIKKTKVVNFATDITIQLTLIFLL
                     IAIVIAPLIAPFVTGYVNANYHPCGNNTGIFPGAIYIKNGMKCNNEYISRKEDSAVK"
     gene            complement(6347..7132)
                     /locus_tag="POB34_003052"
     CDS             complement(6347..7132)
                     /locus_tag="POB34_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDI5919952.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(7460..7536)
                     /locus_tag="POB34_003053"
     tRNA            complement(7460..7536)
                     /locus_tag="POB34_003053"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7500..7502),aa:Asp,seq:gtc)
     gene            complement(7669..8400)
                     /gene="dnaQ"
                     /locus_tag="POB34_003054"
     CDS             complement(7669..8400)
                     /gene="dnaQ"
                     /locus_tag="POB34_003054"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HDI5919953.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVIFAT
                     DDELAAHEARLDLVQKKGGSCLWRA"
     gene            8465..8932
                     /gene="rnhA"
                     /locus_tag="POB34_003055"
     CDS             8465..8932
                     /gene="rnhA"
                     /locus_tag="POB34_003055"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HDI5919954.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(8929..9651)
                     /locus_tag="POB34_003056"
     CDS             complement(8929..9651)
                     /locus_tag="POB34_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDI5919955.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            9685..10440
                     /gene="gloB"
                     /locus_tag="POB34_003057"
     CDS             9685..10440
                     /gene="gloB"
                     /locus_tag="POB34_003057"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HDI5919956.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            10512..11870
                     /gene="mltD"
                     /locus_tag="POB34_003058"
     CDS             10512..11870
                     /gene="mltD"
                     /locus_tag="POB34_003058"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HDI5919957.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(11918..12688)
                     /locus_tag="POB34_003059"
     CDS             complement(11918..12688)
                     /locus_tag="POB34_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDI5919958.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDISHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGFFTSDIIMVDAHKAA"
     gene            complement(12766..13566)
                     /locus_tag="POB34_003060"
     CDS             complement(12766..13566)
                     /locus_tag="POB34_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDI5919959.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            13807..14721
                     /locus_tag="POB34_003061"
     CDS             13807..14721
                     /locus_tag="POB34_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI5919960.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMASAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(14718..15521)
                     /gene="dkgB"
                     /locus_tag="POB34_003062"
     CDS             complement(14718..15521)
                     /gene="dkgB"
                     /locus_tag="POB34_003062"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HDI5919961.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVITALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         4117 a         3821 c         3529 g         4214 t
ORIGIN      
        1 aacccatcgc atcagttgtg atgatttttc ctttaatatc cagcatgtta agaagttctg
       61 ggatagctgt aatctcatta gatttctcat ccgtcctgat ctgtccgatg accagactgt
      121 gcattgttga gaacgcacta atgacatgaa tcgctcccct gcggcgactc ttgtcataag
      181 agtgccggag cgtttttcca tcaattgcga tgacgtcttt atcatctgaa gaatggcagt
      241 cacgcatcca gttaataaag cactcatgaa attttgcagg actgatacag gatacaactc
      301 tggcaatagt atcgtgaaca ggaataccat tttcaaaatc accatattgc ttcaagaaat
      361 cgagatgtgt ttccccgaaa tcctctatat cttcccaact ttctgcacca gaaataacgg
      421 caaaaatagt caatagtagg atgcctgaca atttatgctc tactttccag gcttatctgt
      481 aatcgggaat aatagaaata tgttccatca attttttaag ttccatttgt tcaccttatt
      541 tatgtaagga gtatttgatc atgtataagc aataaaaaac agcttcaggt aatgaggaat
      601 atctcaattt ttaaacataa aatgcgaatt atttagtaca aaaagtgaac ttctgatgat
      661 ctttccctgc gggtcatgtc gccctgccgc gccgccggtt ttccgctcat ggtttattcc
      721 ttttctttta ccttacatgg ccatacaaat agttacctat actgtctttg aatattgccc
      781 taccatttaa agcccaccta ttttttgcga cagcaaaggg atttcttttt taatccaacc
      841 tatcatttca tttttttcaa aacacacgtt aatgccattc acacttccac attcaccaaa
      901 agcatcaaaa taaatttcac atttattatt acctttcgaa atgatactaa ccccccatct
      961 tatattgtta tatttttctt ttctgaaata cttcatttgc aataaatcaa ttatcttacc
     1021 agcataaaga tcgctaattt cattcaccac cactttatat ctgtatattt cattaagacc
     1081 ttccttgcta agcggaacta acgtggtaac atacgatgga agattaatta tctctatcgt
     1141 tttcacgcta tctattccac acaattcatt cactcgcccc atccccagta ccgaaccaga
     1201 aaatagagac aataaaacca tcaaatattt catcataaaa aacctcacta ttctggcgta
     1261 cccgttattt tcatcgtctg caacggtcca tccacccgat aaaaaggcag tccactatga
     1321 gctcctctgg tacactcacc taaagttttc gccgcacgcg attcaattat tgtaataacc
     1381 cttgcctctt ctttattctc atatttctta tctgttcggc ttaaaagagc catgaaattt
     1441 tccttatcaa gaaggtaata ctgcgcatga gcaaactcat gtcccaaacc taatgcaggc
     1501 gattgtgatt tcgagtttaa ttcagttgaa caaaataaag atgctctact attccaatat
     1561 atcgccatat tattcgaatt aaatctaaca ttggttcctt caatatactc tatatttata
     1621 gtttcttcag agcttgataa aaaatctata gtttctttca tttgtgaatc ttgttttaaa
     1681 tactcaacag cctgattaaa atcacttgga tcaccgttaa catggaattt taaacccaaa
     1741 gaatctataa accgaacagg gtttaatgga tatttataca gattccattc acctctcagc
     1801 cctatcgggt cctgggtgat atatctcccc tgcaacggat catagtaccg gttgcggttg
     1861 tagtacagcc ccgactcctc atcatactgc tgtcccggca gacggagtga ctgctgaagg
     1921 tgctgcgcgc tttcctcgcc cagcaggttt ccccactcgt catactcccc ctgccacgcc
     1981 gtttcccctt ccgggctgat aagcgccagc ggcagcccac ggtggtcaca gtggtaaaga
     2041 tgaagtttcc tctccgggat gtattccgcc tccagttgcg cggccatctg ttccgccgtc
     2101 agcccgcact gcgcaagcca ctgctggctt tcttcactca cgctgccctg ccgcagttcc
     2161 cgctccagcc tccccagcat caccgccagc tccgccggta gcgtcacacc cgtgtcctcc
     2221 tgcaacacct ccgccaggct acggtgccgc gccttcgcct gttcaccatt ctctgtttcg
     2281 attctcagga gcggcgtgaa gcttcccggc tggtataccg tctggattct tgtctgttgg
     2341 gtctgtacag tggtcagcct gtcaccgtcc cacccgtacc aggtctcctc cggtttacgc
     2401 gacagcgaca tccaccccgt caggtcacgc tcccgccgcc acacccgttt tcccgtccgg
     2461 cggcccagcg ggtcatagag gtagcggctc tccacctgcg gctcaccgtg ctgtatccgc
     2521 gtgtagaaca ccagacggtg ctggctgtcg tagtggtagt ggtgggtccg ctcgtcgtgc
     2581 atccgtatca ccccttccgg gatgcggtcc gtcttctccg ccagcctgcc gtattcatcg
     2641 tagcggtaga catagtgcgc atcttccgcg atgcggttat ccggccacgc cgtgagggtg
     2701 ctgtccgggt gcagttccgg gtccggcagc cggtttcctg ccgggtccgt cgcatacggg
     2761 atatcgatat ccaggttcgc tgccgtagtg tggacgcccg tcagccttcc cgtgccgctg
     2821 taacggtact cccggcactc ctgcgggccg ctgatgcgca ccagttgtcc gttatcgttc
     2881 cacgtatagt cacagtcaag ctgcggaagg ttcaggtgcc tgctctgtag ctgccctgtg
     2941 agggtgtacg ccgtggcctg ttcgtacccc gctgtactgc ccgccccgcc gaagctacgg
     3001 gccgtctcgc ggtgcagccg gtcccgcgtg tactcgacca gcggcgttcc gcccagcttc
     3061 atccccgcaa gataaccgct gccatacgtc agccactcca ccggcggcag accgtccggc
     3121 tcctgacggg tcgccagccc ctgttcgctg tacgcatgcc ccgtctcatg ctcccacagc
     3181 atctcccctg tctccgggtt ctccaccgtc tgccgttcac ccgtcaggcg gcctttatcg
     3241 tcatagccgt agtggaccga cacgcggtgg ccttcacagg tatggctgag ggtggttaac
     3301 cacccgtgct catcatactg ccactgctct gccgggtcgc cgttcaccgt ccggtgcgtg
     3361 atgcggtccg acgcatcgta gtgccagagg gtgataagcc cctcgtcttc actctgcgtg
     3421 agttttccgg tcaggtcata gtggtaacgt tgcgtccggc cgtcaaaacc gcgctgttca
     3481 gtcagcctgt ccaccgggtc ataccggaac gtggactggc tgccgttctc gttggtcagc
     3541 acggtgatgc gcccggcagc gtcatacccc atgctgcgcg taagaccgcc ttccgtcaca
     3601 ctcaccggac gcccgcgttt atcatattcg atggtgctgc gtttaccgtc cggtgatatc
     3661 gttgctgtca ggtcgcctgc ggcgctgtac tcataacgga tttcacgccc ctgcgcatcc
     3721 ttctgactga ccagttgccc acgcgggtta taactgctgt aagtgctgat gccttcctcc
     3781 cggtgaacgg cgatttgttg accgtaccgg tcgtactcat accgcgtcgt gtaccccgag
     3841 cagtccgtga aggccagcag ttgaccgtaa cggctccatg ccatctgttt tgtactgccc
     3901 gtcgcgtcct gtatccctgt cggcagctca ctcgccgggt catcatagct gtaccgcgtt
     3961 gtctcaccgc tgcgcgaggt ctccgccgtc agccttccct tctcatcata ctcccggctg
     4021 ctgcgaagcc cgtccgggta tcaaaaatgt catctggcag gcggagcggg gactggggca
     4081 ggaaacaccc gttgagtctc tgttgacgca gtaccatgat gcccgtgcgc agaagcagaa
     4141 tacagatacg ctggaaaaac aaattaatga gcgactcgaa ggcgtgttaa gccgctggct
     4201 gctgctgaag aataacgtca tgccagaggc ggcaaccggt accacggccg aaaaataatt
     4261 atttaattaa aggagattat catggcaaat ataagttatt tatctttgag tggagagaca
     4321 caagggctaa tttcagcagg ttgctcaacg cttgattctg tcggaaacaa agcgcagcca
     4381 gaacataaag atcagatcat ggtttatgcg ttaatgcatt ccatctcaag aagtcaaaat
     4441 gtcaatcatc atgaattaat tatcaccaaa ccggttgata aatcctcacc attgctggca
     4501 aaagcactta gtgacaatga aaaaatggca atatgtgaat ttatactcta ccgaaccagt
     4561 aaagcgggta tttaccagcc ttattataaa ataaatctgt caaaagcccg aatatcaagt
     4621 attgattttg tcacaccgca tgctgtcctg gaaaaagaac tagaaccaca ggagagaatt
     4681 gcctttattt atgaagatat ttcctgggag catacgttag ccggtacgaa tgcaatgagt
     4741 aaatggcagg atcgtgtaca gtaacttact gtaataacat aataaattac tattgcggag
     4801 agataaaatg gctggtttaa ggaataacaa caacacacag aatgcacagt gggctgatta
     4861 tgtgggtgac attcttcggg gggctcagcc gataaaccag ctagtacctc agcaccctta
     4921 tcttaacgac gtcccactca ttgatgagtt aaggcatcag aacactcatc atgttattct
     4981 cttgacactt gatgttgcaa aaaaaatact cagcccaata acatcatttg attacattca
     5041 ttttatcact acacatcctt caggtataaa gatacactcg cctggttagt taacgcgagt
     5101 aaattaatga ctgagtttga tgacaatggg aaaattatat ttaaccttaa tgccttaaag
     5161 tatacaaaag caagttactt tgaaatactt ggagagaaat atataaaaat cacaacatcc
     5221 agcccctggc ttttagaaaa gctcggtaaa tatattttct cttcacgagc gcctcaagtt
     5281 ctggaactcg ctattggctg gcgaggagct ttatctgaat caataaaagg agttaagttc
     5341 tgtatctggt tttcagtagc ctggagaacc atagaattta ttatgagttc agagcgagat
     5401 ctggttaatt ttcttggcga tttttcaatg gatgtggcca aagcagttat agccggtggt
     5461 gttgcaaccg ctattggaag cctggcttct tttgcctgtg ttagctttgg ctttccagta
     5521 attcttgtcg gaggagcaat tttactgaca gggatagtgt gtacagttgt tttaaatgaa
     5581 atcgatgctc aatgccattt atcagaaaaa ttaaaatatg caattagaga tggactaaaa
     5641 cggcaacagg aacttgataa atggaaaagg gaaaacatga ctccatttat gtatgttctt
     5701 aacactccac ccgtgatatg aggattaacc atgcgtttac atgttaaatt aaaagagttt
     5761 ctttcgatgt tttttatggc catattattt ttccctgcat tcaatgcttc tttgtttttt
     5821 accggagtta aacctctata ttcaataata aaatgctcca cggaaatatt ttatgattgg
     5881 agaatgttga ttctgtgctt cggtttcacg tcgttttcct ttttaaatat tcatgtaatt
     5941 ttacttacga taataaaatc attcttaatc aaaaaaacga aggtagtcaa tttcgccaca
     6001 gacataacaa tacagctaac tcttatattt ttactaatag ctatcgtaat agcaccttta
     6061 atagctcctt ttgtcacagg atatgtcaat gctaattatc acccctgcgg taataatacg
     6121 ggaattttcc caggagcaat ctatattaaa aatggcatga aatgtaataa tgaatatatt
     6181 tctcgcaagg aagattcagc agttaaataa ttaaatattc taaaacaaac agtttttaac
     6241 tccggattcc atgtcctttc ttgcactgtc tttgtcaaga aaaatttctt atgcaaagaa
     6301 ttttgtatta ttgccgccag aaattcctgg cggcagatat aatttattat tgatagcggt
     6361 taaccaaatc ttttaccatc gctttatagg tcttttgctt aagttgcatg atttgctgct
     6421 ctgtcatatc agtcgtactg gcttcattct tgtcgggcaa tggtggaagc actttatatt
     6481 cataattcac cggcgaccag gtagcccact cacctgttgc cacatccacc agcgtgaaac
     6541 gtaccaggta gcgcagggag atagcgtcgg tgagtttttc atttttatag tcggaccata
     6601 ctgttgattt cagcgcagag tcatattttc ctgtctgcaa tgtatcctgg taaacaataa
     6661 tcgctttctg atgtcctttt gcagccacaa aacgcagtgc ctgcatccag ttcatattct
     6721 cagcactggc aacatcctcg ttctcatttt tatctttgtt tttgttacaa gtcagaggct
     6781 tttgcctgtc cgggatacca gagaatgtgg aaacagtata atattttcgc atctcctcct
     6841 gcataatggt ttccggtgcg cggttgccgg attgaacaag tattacagga gaattaaggg
     6901 gaacgcggaa ctcatctcca tcaagcgcag cttgaatatc ctcttcggat acggccagag
     6961 tggtttcatt accgaataaa tctttgtctg tcagttgctg gggagtgttt ttattaatat
     7021 tactttgtgt gacttgagct tttttcttct tttcatcaat agaagattca gaggcacatc
     7081 ctgcaagcag cgagaataac acacatatgc aacaaagctt ttttgaattc ataattggac
     7141 actccctcgc ctgtcataat gattagaata tttagcattg ataacggact ctaattatat
     7201 acctcactta aataataaca aacaaaatca atttgaaata tacattcatt taatcaatat
     7261 aaatttattc tctggaaata tatttaagct gaatggttcg atatcattat tgaattttaa
     7321 tgaatgagac aacaagcgag aatcacgcac ttacgccaga gtaaaatcat tgaacagaga
     7381 aatggtggaa ttcaaataca aaaaaaccgc aaaattaaaa atcttgcggc tctctgaact
     7441 cattttcatg agtgaatagt ggcggaacgg acgggactcg aacccgcgac cccctgcgtg
     7501 acaggcaggt attctaaccg actgaactac cgctccgcgt tgtgttccgt tgggaacgag
     7561 gcgaatatta cggattgcct cgaccttcgt caacggtttt tctcatcttt tgaatcgttt
     7621 gctgcaaaaa tcgcccaagt cgctattttt agcgcctttt acaggtattt atgcccgcca
     7681 gaggcagctt ccgcccttct tctgcaccag atcaagacgg gcttcatgtg ctgcaagctc
     7741 gtcatctgtc gcaaaaataa cgcgtaactt acttgcctga cgtacaatgc gctgaattgt
     7801 tgcttcacct tgttgctgtt gtgtctctcc ttccatcgca aaagccatcg acgtttgacc
     7861 accggtcatc gccagataaa cttccgcaag gatctgggca tcgagtaatg ccccgtgtag
     7921 cgttcgttta ctgttatcta tttcgtagcg ggcacataac gcatcgaggc tgttgcgctt
     7981 accaggaaac attttcctcg ccaccgcaag gctatcggtg accttacaga aggtattggt
     8041 cttcggaata tcgcgcttaa gcaacgaaaa ctcgtagtcc ataaagccga tatcgaacgc
     8101 tgcgttatgg atcaccaact ccgcgccgcg gatatagtcc atgaactcat cggctacttc
     8161 ggcaaacgtg ggcttatcga gcaaaaattc atcggcaata ccatgtacgc caaaggcttc
     8221 aggatccacc agccgatcgg gtttgagata aacatggaag ttattgcccg tcaggcgacg
     8281 gttcaccact tcaacggcac caatctcaat gatcttgtgg ccttcatagt gcgcaccaat
     8341 ctggttcata ccggtggttt cggtatcgag aacgatctgg cgtgtaattg cagtgctcat
     8401 agcggtcatt tatgtcagac ttgtcgtttt acagttcgat tcaattacag gaagtctacc
     8461 agagatgctt aaacaggtag aaattttcac cgatggttcg tgtctgggca atcccggacc
     8521 tgggggttat ggcgctattt tacgctatcg cggacgcgag aaaaccttta gcgctggcta
     8581 cacccgcacc accaacaacc gtatggagtt gatggccgct attgtcgcgc tggaggcgtt
     8641 aaaagaacat tgcgaagtca ttttgagtac cgacagccag tatgtccgcc agggtatcac
     8701 ccagtggatc cataactgga aaaaacgtgg ctggaaaacc gcagacaaaa aaccagtaaa
     8761 aaatgtcgat ctctggcaac gtcttgatgc tgcattgggg cagcatcaaa tcaaatggga
     8821 atgggttaaa ggccatgccg gacacccgga aaacgaacgc tgtgatgaac tggctcgtgc
     8881 cgcggcgatg aatcccacac tggaagatac aggctaccaa gttgaagttt aagcctgtgg
     8941 tttacgacat tgccgggtgg ctccaaccgc ctggcgaatt cgtggcttgt ttttactctg
     9001 tttcatcgga tttagcgtta aaggaatagt ccgtttccgg gcaacaataa gttgtaagca
     9061 accaagcgca ggaatatgcg cattcaatag ttttcctccg tgtttgttcc acgggagaac
     9121 gtggaaacgg ctggcgtgta gcacttcaaa attcaacaaa gagagccagt ccagctgccg
     9181 catcagagta aacatccggc tgttataggg cgaggttttg cgcaataccg gcacaagttt
     9241 gcgtaatccc attaaactga tgggattgaa gccactaatg accagccagc catcatcaat
     9301 caatacccga tcggcttcac gcaataaacg atgcggatcg gtgcaccacg gcaatgtatg
     9361 tgccagtaga caaacatcaa cggatttatc ggcaaaagga agatgaagtg ggtccgcctg
     9421 tacctggacg ggcattcctt gcgcagaaac attcacttga tgagaaaccg cgcacgcttc
     9481 gcaattgatt tctgcgctta aattgccaat cttaagcaga tgaaaaccat acattttagt
     9541 gaaccacggg ttgagctggc gctccagcgc cttgcgataa agctctcccc agggcaaatc
     9601 gccccagcaa tcaggagcca cgacagtttg agggactctt gccggtttca tcacaacctt
     9661 ccgtttcaca ctgagaggta atctatgaat cttaacagta ttcccgcctt tgatgacaat
     9721 tacatctggg ttttgaatga tgaagcaggt cgctgcctga ttgtcgatcc cggagacgca
     9781 gagccagtat taaacgccat tgccgccaat aactggcaac cagaggccat atttctcacc
     9841 caccatcacc acgatcacgt tggcggcgta aaagaactgg tggaaaagtt tccgcaaatt
     9901 gtggtgtatg gtccacaaga gacacaagat aagggaacaa cacaggtagt caaagatggc
     9961 gaaactgcct tcgttttagg gcatgaattt agtgtaattg ccacaccggg tcacacttta
    10021 ggacatatct gttacttcag caaaccttat ctattttgcg gcgacacgct gttttctggt
    10081 gggtgtggtc ggttgtttga agggacacca tcacaaatgt atcaatcact taaaaagtta
    10141 agtgcgttac ctgacgatac attggtatgt tgtgctcatg aatatacgtt atcaaatatg
    10201 aagtttgctt tgagtattct tccgcacgat ttgtccataa atgattatta tcgtaaagtt
    10261 aaggagttac gggcaaaaaa tcaaataaca ctacccgtaa ttctgaaaaa tgagcggcaa
    10321 attaatgttt ttttaagaac agaagatatt gatttaatta atgtaattaa tgaagaaaca
    10381 ttattgcaac aacctgaaga acgttttgca tggttaaggt caaagaaaga taggttctga
    10441 taaaactttc ttgtcatcgg ctccgttcgc cgttatgatc ggtcgtcttt taagcaacta
    10501 ttgacacaca catgaaggca aaagcgatat tactcgcctc tgtcctgctc gtgggctgcc
    10561 aaagtaccgg caacgttcaa cagcacgcac agagcctttc tgcagctggt caaggggaag
    10621 cagcaaagtt tacaagtcag gcacgatgga tggacgatgg gacgtctatc gcgccagatg
    10681 gtgacttgtg ggctttcatt ggcgacgagc taaagatggg aattccggaa aatgaccgga
    10741 ttcgcgaaca gaaacagaaa tatttacgca ataagagcta tctccacgat gtaactttac
    10801 gggcagagcc gtatatgtac tggatagccg ggcaagttaa aaaacgtaac atgcctatgg
    10861 aactggtact actacccata gtggagagcg cttttgatcc tcacgcaacg tctggcgcca
    10921 atgccgcagg catctggcag atcattccga gcacggggcg caattatggt ttgaaacaga
    10981 cccgcaatta tgacgcgcgt cgcgatgttg ttgcttcaac aactgccgcg ctgaacatga
    11041 tgcagcgtct gaacaagatg tttgacggcg actggcttct gaccgtagcg gcttataaca
    11101 gcggcgaagg tcgggtcatg aaggcaatta aaacgaacaa agcgcgtggg aaatccacgg
    11161 acttctggtc gttaccgttg ccgcaggaaa cgaagcagta cgtgcctaaa atgctggcat
    11221 tgagtgatat tctcaaaaac agcaagcgtt atggcgtacg tctgccaacg accgatgaaa
    11281 gccgtgctct ggcgcgtgtg cacctgagca gcccggttga aatggcgaag gttgcagata
    11341 tggcggggat ttccgtcagc aagctgaaga cattcaacgc tggcgtaaaa ggctccacgc
    11401 tgggcgcaag tggcccgcag tacgtgatgg tgccaaagaa gcatgcagat caactgcgtg
    11461 aatctctggc ttcaggcgaa attgctgctg tacagtcgac gctggttgcc gacaatacgc
    11521 cgcttaacag ccgtgtttac accgtacgct ctggcgacac gctttcaagt atcgcttcac
    11581 gtctcggcgt aagcaccaaa gatttgcagc agtggaacaa actgcgcgga tctaagctga
    11641 agccaggcca aagtttgacg attggtgcag gcagtagcgc acagcgactg gcaaacaaca
    11701 gcgatagcat tacgtatcgt gtgcgcaaag gcgattcgct ttcaagcatt gctaaacgcc
    11761 acggcgtgaa catcaaagat gtgatgcgct ggaacagcga cactgcgaat ctgcaaccag
    11821 gcgataagct gacgttgttt gtgaaaaaca acaacatgcc agattcctga caaaccagat
    11881 aataaaaagg caccgattcc cccggtgcct tttttattta tgccgcttta tgtgcatcta
    11941 ccatgatgat atcactggtg aaaaagccat cattctgcaa ggcaaaatac gttctcacct
    12001 ctgtcgatgc gctctgttgg taaatgcgaa tagcgtctac taacgcttct ggcgtacgca
    12061 ttctcgcgac ccatgaagaa aattccagcg gtaacttatc tgtaattaaa ttatcaacta
    12121 tcagattggc ttcattgatt aacgtcaacc actcaccgct ggcgtagttt cgtacgtgag
    12181 agatatcgcg taatgcttct accgtctgta accagatgtc gcgcactggg tgacccggag
    12241 acattacgtc catcacaatc agcctaccgc caggtttcaa tatcctattc acttctcgca
    12301 gtgctgcacc aacatcatgc cagtgatggg cagaataacg gctgataaca atatcaaatg
    12361 cgttatcggc aaatggcaga ctttcggcat atccctggcg ggtggtgata tttttcagtt
    12421 gccgggcttc ggcagcttgt gccacgacat ccagcatatg ggcagataag tcatacgcca
    12481 ccaccgtgct cacgttttgc gcagcgacaa agctggcatg ccctgctccg cagcccatat
    12541 caagtacact tgcaccagga taatcagcca gacgcaccgc caggcgctgt aaatctcgtc
    12601 cggatgcatg tacggtactg gtcagatatt cgcaggcttg agagctaaac tgcttttcta
    12661 cgtggtcatg gtgggattgt gttgtcattg cgctgtcctt ttgttgtttg agtataaaag
    12721 gacagcgcca aacgttggtc tgccctgtgg cagacctgac ataccttatt tatcaggctt
    12781 gccgggactg aattcaacga gtagcggatt gtgatcggaa gcgcgcgtaa ccaatacaga
    12841 agcttcgctg acgttcagac cacggtagaa aacaaaatcg agcgggcggc caaacgcccg
    12901 acggcgctga tcatcggtaa aacgcacctg gcgcagcgac atttcccgcg caaagcgata
    12961 taacgcgttc atccttctac ggctccaggc attgaaatct cccgccataa tgaccgggcc
    13021 gctgtggtga gctatctgat caccaatagg aagtaactgc ttactataga catccacgcc
    13081 cagactgaag ttgacggcgt gtatattaac caccatcaac aggcgggtgt caggtaatgg
    13141 atagaccgtc accagtgccg acttcgccag acgcaaaatg ggttctcgtt cacgtaacgg
    13201 gcagcaatac actggatgcg ccgccgaaag ggtcattaca ccggaaggat gttgtggcag
    13261 cacgaaagcg ggcacctgat cggcggcaag atagttagcg gtcgcaaact gtactaactc
    13321 tggcgttgtc tgcgcttcct gcaataacac cagatgtgca tctttgccgt agttctttaa
    13381 taccgacaac cattcagcgc gttgctgttt gtatatgttc cacaccagga tccgaatacg
    13441 ctcttcggta cttaacggtt ctccaggtgg taatgcctgg ccgatgctcg caaaagaccc
    13501 cggcggtaag atcctttccg caggttgtcc ggcaacatag cgcatggcat aggtgttttt
    13561 tcgcactttt gactttgaaa cctctattac gtcaaccctt ccgcactcgg gagggttcta
    13621 ctgttatagg gattttagct cactctttca acgagcaatt gtatattgtt atgtaagcaa
    13681 gtgcttacac cggtcagaat tttgctcatt atggagaaca ttaaatcgca tgacatatca
    13741 cgcatccaga tggtttaatg acaaataaat tcaaaatatc tcttctattt tgcaacagga
    13801 gcaaaaatga aagccacgtc ggaagaactc gccatttttg tttcggtcgt cgaaagcggc
    13861 agctttagcc gggcagcgga acaattaggg caagcaaact cagcggtaag ccgggcggtg
    13921 aaaaagctgg agatgaaact tggcgttagc ctgcttaatc ggaccacgcg acaacttagc
    13981 ctgacggaag aaggcgagcg ttattttcgt cgcgtacagt caattttgca ggagatggca
    14041 tcggcagaat cagaaattat ggagacgcgt aatacaccgc gtggactgtt acggatcgat
    14101 gccgcaactc cagtggtgct gcactttctg atgccgttaa ttaagccttt ccgtgaacgc
    14161 tatccggaag tcactttgtc gctagtctcc tccgaaacga ttattaattt gatcgaaaga
    14221 aaagtggatg tcgcgatacg cgctggtacg ttaacggatt ccagcttacg tgccaggccg
    14281 ttatttaaca gttatcgaaa aattatcgcc tcccccgatt atatttcccg ctacgggaag
    14341 ccagaaacga tcgacgattt aaagcaacat atttgcctgg gattcactga acccgcttcc
    14401 ctcaatacct ggccgatagc ccgtagcgat ggacaattac atgaggtgaa gtacggtttg
    14461 tcatccaata gtggggaaac actgaaacag ctttgcctga gtggcaacgg gattgcgtgt
    14521 ttgtccgact acatgatcga caaagaaatc gctcgcggag aattggtgga gttaatggca
    14581 gataaagtgt tgccagtgga aatgccattc agtgccgtct attacagcga ccgtgcggta
    14641 agtacgcgca tccgggcttt tatcgatttc cttagcgagc atgtaaaaac agctcccgga
    14701 ggagctgtca gagaggctta atcccattca ggagccagac cttccgggct aaccaggcgg
    14761 tcgttgcaat ccagtgcggc gatcgctttt ttatcttcgg catcaagctg taaattttgt
    14821 gccttaagat tactttccag gtttttacgt ttagtagaag aaggaattac tgagtaacct
    14881 tcccccatag cccacgccag aatcacttgt gccggagtcg cattgtgttt agctgcgata
    14941 cgagcaataa cctcatcttt cagggcctta ccatacgcca gcgtcatata ggaagtaata
    15001 tggatgccgt gctgtttagc ccaggcaacc actttacggt tttgcagata aggagagagt
    15061 tcaatctggt tagtagcgat gttttcagca ccaacagcag caatcgcttt ttccatcaac
    15121 gggatcgtga agttggaaat accgatctca cgcgtcagcc cttgtttttt ggcttccagc
    15181 agcgcctgca taaactcttc aacagagact tcatcgtttg gtgacggcca gtggattagc
    15241 gtcagatcaa cataatcggt acgcaatttt tgcaggctct ctttcagact tgggatcaat
    15301 ttgtctttgc tgagattttc aatccagatt ttagtggtga tgtagagttc atgacgtggc
    15361 acgccacttt ctgcaatcgc ctgacctact gcggcttcgt tatcatagat ttgtgcggta
    15421 tcaattgcgc gataaccaag ttcaagcgcc gttatcacag atgaaataac aacgtcgtct
    15481 ttcagacgga aagtacctaa accaaatgca gggatagcca taatatgcct cttttaatgc
    15541 tattttcgtt aactgaggtg attatgggct tcagactgtt gaggaaaaag agcgtaaaat
    15601 gcagaggatt tttgcgattc tggcaataat agatatacag acgaaaaaaa acctcgagcg
    15661 ttaactcgag gcttcttaat a
//
LOCUS       DAMZET010000096        15478 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.096, whole genome shotgun sequence.
ACCESSION   DAMZET010000096 DAMZET010000000
VERSION     DAMZET010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.096"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            298..1212
                     /gene="sitA"
                     /locus_tag="POB34_003063"
     CDS             298..1212
                     /gene="sitA"
                     /locus_tag="POB34_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HDI5919962.1"
                     /translation="MLSIKKVTMLLGCLVLTCSIAFQASAAEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDAFIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQIAELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            1212..2039
                     /gene="sitB"
                     /locus_tag="POB34_003064"
     CDS             1212..2039
                     /gene="sitB"
                     /locus_tag="POB34_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HDI5919963.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPGGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREER"
     gene            2036..2893
                     /gene="sitC"
                     /locus_tag="POB34_003065"
     CDS             2036..2893
                     /gene="sitC"
                     /locus_tag="POB34_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HDI5919964.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQLTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLMIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            2890..3747
                     /gene="sitD"
                     /locus_tag="POB34_003066"
     CDS             2890..3747
                     /gene="sitD"
                     /locus_tag="POB34_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HDI5919965.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKSWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQASGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISILVSMTGVWLSFFIDSAPAPTIVVLFAVMFIMTFTVTSINARTKENA
                     EPRDLLSSD"
     gene            complement(3981..4238)
                     /locus_tag="POB34_003067"
                     /pseudo
     CDS             complement(3981..4238)
                     /locus_tag="POB34_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            4236..4649
                     /locus_tag="POB34_003068"
                     /pseudo
     CDS             4236..4649
                     /locus_tag="POB34_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015377870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
     gene            4990..5370
                     /gene="crcB"
                     /locus_tag="POB34_003069"
     CDS             4990..5370
                     /gene="crcB"
                     /locus_tag="POB34_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HDI5919966.1"
                     /translation="MIKSLFAVIIGGSVGCTLRWLLSTRFNSLFPNLPPGTLVVNLLA
                     GLIIGTALAYFLRQPHLDPFWKLMITTGLCGGLSTFSTFSVEVFALLQAGNYIWALTS
                     VLVHVIGSLIMTALGFFIITILFA"
     gene            complement(5750..6943)
                     /locus_tag="POB34_003070"
     CDS             complement(5750..6943)
                     /locus_tag="POB34_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5919967.1"
                     /translation="MSSKIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISLTGSKTVVLWALGGYGVSFTLLGLGSVLM
                     ATGAVTTAVGLGILIIARIVYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLVIHPLAPVWMLMAAPVWAVVMLLRLPGTPPQPTPERKSVSLKRDC
                     LPYLLCAMLLAAAMSMMQLGLSPALTRQFDTDTTTISQQVAWLLGLSAIAALIAQFVV
                     LRPQRLTPVALLLSAGVLMSSGLAIMLTEQLWLFYLGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLAASHTLGYGLCALLVPLASKTGVSIALIVTALFAAVLFTIVSACIWH
                     YRTIK"
     gene            7079..8803
                     /gene="iucA"
                     /locus_tag="POB34_003071"
     CDS             7079..8803
                     /gene="iucA"
                     /locus_tag="POB34_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HDI5919968.1"
                     /translation="MILPSEKSATDVAAQCFLNALIRETKDWQLAEYPPDELIIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPAHLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESYAHTQQTIDARHDWAILREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RQEAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQVWCQALFAKGLIRDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSVRTLSVKEVERGMRLARLAQTDGWQMLQARFPTFRVMQEDGWAGLRDL
                     NGNIMQESLFSLRENLLLEQPQSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATEWLDTIDEAQAENIFTREQLLRYFPYYLLVNSTFAVTAALGAAGLDSEANLM
                     ARVRTLLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            8804..9751
                     /gene="iucB"
                     /locus_tag="POB34_003072"
     CDS             8804..9751
                     /gene="iucB"
                     /locus_tag="POB34_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HDI5919969.1"
                     /translation="MSGANIVHSGYGLRCEKLDKPLNLGWGLDNSAVLHWPGELPTGW
                     LCDALDQIFIAAPQLSAVVLPWSEWCEEPQALTLFGQVQSDIIHRSAFWQLPLWLSSP
                     ANRASGKMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQIAYLERQLTSKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGVTHYLLLNEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            9751..11493
                     /gene="iucC"
                     /locus_tag="POB34_003073"
     CDS             9751..11493
                     /gene="iucC"
                     /locus_tag="POB34_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HDI5919970.1"
                     /translation="MNHKDWDLVNRRLVAKMLSELEYEQVFHAESQGDDRYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCADEPVLAQTLLMQLKQVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLNADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNEMDIHQLLTAAMDPQEFARFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEEFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMKKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLED
                     LQNPLWLVTQEYES"
     gene            11490..12767
                     /gene="iucD"
                     /locus_tag="POB34_003074"
     CDS             11490..12767
                     /gene="iucD"
                     /locus_tag="POB34_003074"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HDI5919971.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELDCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKRRLFLVQTSQGEYFARNICLGTGKQPYLPPCVK
                     HMTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLEEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFVVNASMQTHGIAEPQLSL
                     MAWRSARILNRVMGRDLFDLSMPPALIQWRSGT"
     gene            12849..15050
                     /gene="iutA"
                     /locus_tag="POB34_003075"
     CDS             12849..15050
                     /gene="iutA"
                     /locus_tag="POB34_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HDI5919972.1"
                     /translation="MMISKKYTLWALNPLLLTMMAPAVAQQTDDETFVVSANRSNRTV
                     AEMAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSRSRTNYGMNVRGRPLVVL
                     VDGVRLNSSRTDSRQLDSIDPFNIDHIEVISGATSLYGGGSTGGLINIVTKKGQPETM
                     MEFEAGTKSGFSSSKDHDERIAGAVSGGNEHISGRLSVAYQKFGGWFDGNGDATLLDN
                     TQTGLQYSDRLDIMGTGTLNIDESRQLQLITQYYKSQGDDDYGLNLGKGFSAIRGTST
                     PFVSNGLNSDRIPGTERHLISLQYSDSAFLGQELVGQVYYRDESLRFYPFPTVNANKQ
                     VTAFSSSQQDTDQYGMKLTLNSKPMDGWQITWGLDADHERFTSNQMFFDLAQASASGG
                     LNNKKIYTTGRYPSYDITNLAVFLQSGYDINNLFTLNGGVRYQYTENKIDDFIGYAQQ
                     RQIAAGKATSADAIPGGSVDYDNFLFNAGLLMHITERQQAWLNFSQGVELPDPGKYYG
                     RGIYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGNNLRTQIAAYYSISDKS
                     VVANKDLTISVVDDKRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGTWQKYDV
                     KTASPSKATAYIGWAPDPWSLRVQSTTSFDVSDAQGYKVDGYTTVDLLGSYQLPVGTL
                     SFSIENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         3722 a         3891 c         3961 g         3904 t
ORIGIN      
        1 cccttcattt agtgactgcc cctgggtttt tcatgctctc ctgcgtccta gagaacttgc
       61 caataaccta cattattttt gctttgccat taccaatcgt cctgaacaag ttcattttaa
      121 agccaaagcg ctgcccaact tttatcctca ataccacatc gtcagcagga aataacctaa
      181 tccctgtgtt gggatcttat aatcgctaat gataattatt tacattttat agcttctgct
      241 ataaaacata gtagttgcta tgtttattct aattttgctc actataggta ctaaatcatg
      301 ctctcaataa aaaaagtaac catgctcttg gggtgcctag tcctcacctg ctcgatagca
      361 tttcaggcga gtgcagctga aaaattcaag gtcattacaa cttttaccat catcgcagat
      421 atggccaaaa acgtggctgg agatgctgca gaagtctcat ccataaccaa gcctggtgca
      481 gaaattcatg agtatcagcc tacccctggc gatattaaac gtgcgcaggg ggcacaactg
      541 attctcgcca atggtatgaa tctggaattg tggttccaac gcttttacca gcatctcaat
      601 ggggttccag aagtaattgt ctcttcgggt gtgacgccag tagggatcac cgaagggccc
      661 tatgagggca aacctaaccc ccatgcctgg atgtcgccag ataatgctct gatttacgtc
      721 gataatattc gtgatgcgtt tataaaatac gatccggcaa atgcacaaac ctaccaacgt
      781 aatgccgata cttataaagc caagattacc caaacccttg cccccctgcg taagcagatt
      841 gcggaactcc ctgagaatca gcgatggatg gtcaccagtg aaggggcttt ttcttatctc
      901 gcacgcgatt tggggctaaa agagctttat ctgtggccga ttaatgccga tcaacaagga
      961 acaccgcagc aggtacgtaa ggttgttgat atagttaaga aaaatcatat cccggcagtc
     1021 tttagcgaga gtacgatttc cgataaacca gcgcgtcagg ttgcgcgtga aaccggcgcg
     1081 cactacggtg gtgtgctcta tgtcgattcc ctgagcacag aaaacggccc ggtacctacg
     1141 tatatcgacc ttctgaaggt tactaccagt acgctggtac agggaattaa agccggaaaa
     1201 agggagaaat aatgatgcaa tctgcaggca ttgtcgttaa tgatgttact gtcacctggc
     1261 gtaacgggca cacagcactg cgcgatgcat ccttcacagt accaggcgga tctattgccg
     1321 ctctggttgg ggtaaacggt tccggaaaat cgacgttgtt taaagcgatt atgggatttg
     1381 tgcgtctgac cagcgggaag atatctgttc tgggtattcc cacacgacag gcgctacaga
     1441 aaaacctggt tgcctacgtc cctcagtcag aagaggttga ctggtcattt cctgtactgg
     1501 tggaagatgt ggtgatgatg ggccgctatg ggcatatggg tatgttgcgt atcgccaaaa
     1561 agagggatcg tcagattgtc accgatgcgc tggaacgtgt cgatatggtg gattttcgcc
     1621 atcgacaaat cggcgagctt tccggtggac aaaagaaacg cgtctttctg gcgagagcga
     1681 ttgcacagca gggtgatgtg atcctgctgg atgaaccttt taccggcgtt gatgtgaaaa
     1741 ccgaagcgaa aatcatcagc ctgttgaggg agttacgcgc ggaaggcaaa acgatgcttg
     1801 tctcaacgca caatctcgga tccgtcacga cattttgcga ttatacggtc atggtcaaag
     1861 gcaccgtcct ggcaagcggg ccgacagaca ccacttttac agccgagaac ctggagctgg
     1921 cttttagcgg cgtactgcgc catgtcacac ttaacggctc ggaagaaagc attattactg
     1981 acgatgaacg cccttttgtg gcacatcgac cgtcagcggt gcagagggaa gaaagatgaa
     2041 cgtgctactg gaacccttca gctatgaata tatgctcaat gcgatgtggg tctcggcgat
     2101 ggtcggcggt ctctgcgctt tcctgtcatg ctatctgatg ctaaaaggct ggtcgctgat
     2161 tggcgatgcg ctgtcgcact ctattgtccc gggtgttgcg ggggcatata tgctggggct
     2221 tccgttttct ctgggagcct tcttttccgg cggactggca gcgggcagca tgctgtttct
     2281 taaccaactc actcgcttaa aagaagatgc catcatcggc ctgatcttct cgtccttttt
     2341 tggcctgggg ctgtttatgg tgtcattgaa cccgacatcc gtgaacattc agaccatcgt
     2401 cctgggcaat attctggcaa tcgatccggc ggatatcctg caactcacta tcattggcat
     2461 tctctcgatc atcgtgcttt ttttcaaatg gaaagatctt atggtgacct ttttcgacga
     2521 gaatcacgcc cgtgctattg gattgcatcc gggcagatta aaaatcctct tcttcaccct
     2581 gctgtcggtt tcgaccgttg cggcgctgca aacggttggt gcctttctgg tgatctgtct
     2641 ggtggtgact cccggtgcaa cagcatggtt gttaaccgat cgttttcccc gcctgctcat
     2701 gattgctgta actattggca gtgtgaccag tttcctcggc gcgtgggtca gttactttct
     2761 ggacggcgcg acaggtggaa tcatcgtggt ggcgcagaca ctgctgtttc ttttagcatt
     2821 tgtctttgcg cccacccatg ggcttttggc aaatcgtcgg cgtgcgcata aagcgctgga
     2881 ggatcgctca tgatggcatt attactggag ccactgcagt tcacctttat gagtcacgca
     2941 ctgctgattt cactggtagt ttccataccc tgtgcgctgc tgtcggtctt tctggtcctg
     3001 aaaagttggg cactgatggg tgacgcgatg agtcatgcgg tttttcctgg catcgtgctt
     3061 gcctggattc tggggttacc tctggctaca ggggcattcg tcgccggagt attctgtgcg
     3121 gtggcgacgg gatacctgaa agacaacagc cgaatcaagc aagatacagt gatggggatt
     3181 gttttttctg gcatgtttgc cgcaggtctg atcttgtata ttgcagtcaa accagacgta
     3241 catcttgatc atattctttt cggcgacatg ctcgggataa ccatcggcga tataatccag
     3301 acggtgatta ttgccggact ggttacactt gttattagcg taaaatggcg ggattttttg
     3361 ctgttcagct ttgattatca acaggcgcag gcaagcggtt tgcatacgag atggctgcat
     3421 tatgggctgc tgtgcatggt ctccctgacc attgtggcga cgctgaaagc ggtaggtatc
     3481 attctctcta tttctcttct cattgcgcct ggtgccattg cggtactcct gacacaacgc
     3541 tttcatattg cgctcctgct ggcgactggc atatcgatat tggtctcaat gactggtgtc
     3601 tggctgtcct tttttatcga cagcgcgcca gctccgacaa ttgttgtctt attcgcggtc
     3661 atgttcatca tgacgtttac cgttaccagc atcaacgcac gcacaaagga aaatgccgaa
     3721 ccacgggatt tgttatcatc cgattagcag aacactcggc cagcaacatc cgcttattgg
     3781 cgatgcgctt gaacgaataa tgcacagact tatgattctg ataagttttg ttgccagaga
     3841 gttgctgcgg actggatagc aagtaatgtc gattctcagg ggtagcaaac agcgcagtaa
     3901 aatgagggaa gtagtggcaa aacatagaga aaattggggg gcgagggcca gtgatgtaaa
     3961 aaaccacgct aagagtttaa ttaattgttt gtattgaagt ttaatgatct taactcaccc
     4021 ttaagaatat cttccggtaa cgaagacgta taatgcccca gcatatttat atggctatgt
     4081 atcagcagcg ataatcttgc cggatgctac tcctcaggag tttctcccga tttgcgaaga
     4141 tgtgacaacg cctcctgcat gtaaagtgtg tttcacagca caaccgcatt ggtcaccagc
     4201 cccagagcac ctagttgatc ttcctgccct taacggtagc tgaagatgac tgggctggat
     4261 ggaaaatcct gcatggtgca ttgggagagc agatagaatt ggttggcgac gatctgtttg
     4321 tgaccaacgt aaagtatatt cagcgcggca ttgacgaaaa ccttgccaat tcagcattga
     4381 ttaaactcaa ccagattggt tccctgagtg aaacctttga tgcggtacaa ctttgccatg
     4441 acaacaactg gggcaccttt atctcacacc gcagtggtga gacagtggac agctttatcg
     4501 ccgatatgac agttgcaatg cgcgcaggtc atctcaaaac gggagctcca tgccggggtg
     4561 agcgtattga aaaatataac cagcttatga gaattgagga tgaactgggt tcatcggcgc
     4621 aatttgctgg aaagtctgcc tttaaatgac attattgtgc caatccccca ggcttacacg
     4681 tcggggttat tctggaggaa tgatgaaagc tcacgttttt gttatttcta tgcttactgg
     4741 cattgtcgtc acctatgccg tgttacttct tggttgcttg ttcatcgata aaacgctccc
     4801 cactgtagat gttgtcattc tgtcacttgt cgtgggggct tctgctcagc aattgtcgag
     4861 ggtattaatg tcaataaata gaaccttccc ttatcttgca tgtagtcaaa ggatataaat
     4921 taacaatatc agtcaaatga gaacatattc tgatattgtt acgataaaaa attattttat
     4981 gaggggtaca tgattaaatc actttttgcc gtcattatcg gcggttctgt tggatgtact
     5041 ctgcgctggt tactgtccac acgctttaac agtctttttc caaatctacc tccaggtaca
     5101 ctggtggtaa acctccttgc cgggttaatt attggtactg cgttggcgta ctttttacga
     5161 caaccacacc ttgatccttt ttggaaatta atgataacaa cgggactttg tggaggtctg
     5221 agtactttct ctacattttc tgttgaagta ttcgcattac tccaggcggg aaattatata
     5281 tgggctttga cgtctgtact tgttcacgtc attggctccc tcatcatgac agctttaggc
     5341 ttttttatca taaccatact tttcgcatga gaattcatta acgttacagg tttatcaact
     5401 aatgtgacgc ttaggataga atttcgctcc atggtatgcg tttgcagtca ttacagtctc
     5461 catgacgact gtaatgactg caaacgcatt acctgacata tcagtataaa caatgaatta
     5521 acctcttggc gtatttttta catcactggc cctcaccccc caaaattctc tctgaaaaat
     5581 tgttgcggtt aacgttgcca atgtatccac ccatttcaga agacgatctg gcatcctggt
     5641 ggcatgctgc gatgtgcttc aggtgagtgc actgggcggt cagcacaggc ggtttcgaca
     5701 gcgaaattgc acaggacgtt atgccggctg tgagtacaac atcatagcct tacttgattg
     5761 tacggtaatg ccaaatacat gcagacacta tggtaaataa tacagcggca aataatgccg
     5821 tcacaatcag cgcgattgag acacctgttt tcgacgccag tggcaccaac aatgcgcaca
     5881 gcccatagcc aagtgtgtga ctggccgcaa gccagcccgc gcctgcgccg tcggccagct
     5941 tatcattcag taaaagttga taagcgggtg ttgccagagc ggccccaaat gacagcactg
     6001 cacagcccag gtaaaacaac catagctgtt cagtgagcat gatagccaga ccactactca
     6061 tcaacacccc ggcactcagg agcagagcca ctggagtcag gcgctgtgga cggagtacca
     6121 cgaactgcgc gataagcgca gctattgcgg acagccccaa caaccacgcc acctgttggc
     6181 taatagtggt ggtatcagta tcgaactggc gagtaagggc tggcgaaagt ccaagctgca
     6241 tcatgctcat tgccgcagcc agtaacattg cgcaaagcag ataaggcagg caatcccgct
     6301 tcaggctgac gctcttgcgc tccggtgtgg gctgtggtgg tgtgccgggc aaccgcagaa
     6361 gcatcaccac agcccacacc ggagctgcca tcagcatcca cactggcgcc agagggtgaa
     6421 tgaccaacat tgccgccgcg cacagcggcc cgaatagcct gccgcagctc aggccggagc
     6481 tgatggttgc cagagcggcc atgcgattcc cttctcccgc tctctgcaat gcccagacct
     6541 gacaggctgg caccattgct gacacggtca gaccgtagac gatccgggcg atgattaata
     6601 ttcccaaccc caccgctgtt gttacggcac cggtagccat cagcacactt cccagcccaa
     6661 gcagggtaaa gcttacgcca tatcctccca gagcccacag caccacagtc ttactgccag
     6721 taagggaaat ttgctttccc caccatgggg aagatggcag aaacagcatt gagcccagca
     6781 tcagcagggc agcccataca gacagactca gatttgtctg tatgaccaga acagggagta
     6841 caaccagcag accgttctgt ccgataccga gaagaccggc actgaacgcc agtggccagc
     6901 aggacagggt tttttggggc gtatcttcga tcttagatga cattgtaatg tttcaacacc
     6961 atgtattaat tgtgtttatt tgtaaaatta atttttctga caataacatt tctcattgat
     7021 aatgagaatc attattgaca taattgttat tattttactg tgtaggagct gtttgattat
     7081 gatcctgccc tctgaaaaat ccgccacaga tgtggctgcg cagtgcttcc tgaatgcctt
     7141 gattcgtgaa accaaagact ggcaactggc ggaatacccg ccagacgaat tgattatccc
     7201 gctggacgaa cagaagtcgc tccatttcag agtggcttat ttctccccaa cccagcatca
     7261 ccgctttgca tttcctgctc atctggtcac ggcatcaggc agttatcctg tcgactttac
     7321 cactctctcc cggcttatta ttgataagct ccggcatcaa ctttttctgc cagttcccct
     7381 ctgcgaaact ttccaccaac gcgtgctgga aagctacgcc catacgcaac agacaattga
     7441 tgcccgccat gactgggcca tcctgcgtga aaaagcgttg aattttggcg aggctgagca
     7501 ggcactgctg acaggacacg ctttccaccc tgcacctaag tctcatgaac cgtttaaccg
     7561 ccaggaagct gaacgatacc tgcctgacat ggcacctcac ttcccgctgc ggtggttttc
     7621 ggtggataaa acgcaaatcg ctggtgaaag tctgcatctt aaccttcaac agcggttgac
     7681 gcgatttgcc gcagaaaatg cgccccagtt actcaacgaa ttaagtgaca atcaatggct
     7741 gttcccgctg cacccgtggc agggagaata tcttttgcag caagtgtggt gccaggcact
     7801 ttttgctaaa ggacttatca gagacttagg cgaggccggc acgtcgtggc tgccgaccac
     7861 ctcttcccgc tccctctact gtgctaccag ccgcgatatg atcaagttct ccctgagcgt
     7921 tcggctgacc aactccgtcc gtactctgtc tgtgaaagag gtggagcgag gaatgcgcct
     7981 ggcacgtctg gcgcaaaccg acggctggca gatgctacag gcccgcttcc ctactttccg
     8041 ggtaatgcag gaggacggct gggccgggct gcgcgatctt aacggcaaca tcatgcagga
     8101 aagtttgttt tccctgcgtg aaaacctgct gctggagcag ccgcaaagcc agaccaacgt
     8161 tctggtttcc ctgactcagg ccgcaccgga tggcggtgat tcgctgctgg tttcggcggt
     8221 aaaacgcctg agcgatcgcc tcggtatcac tgtgcaacag gccgcccacg catgggtcga
     8281 tgcttactgt cagcaggtgc tgaagcctct gtttacggct gaagcggatt acggcctggt
     8341 gctgctggcg catcagcaaa atattcttgt gcagatgctt ggggatctgc cggtcggatt
     8401 tatttaccgt gactgccagg gcagcgcgtt tatgcctcac gcgacagagt ggctcgatac
     8461 cattgacgag gcgcaggcgg aaaatatttt cacccgtgag cagttgctgc gctatttccc
     8521 ttattacctg ctggttaact ccacttttgc cgtaactgct gcgctgggtg ctgccgggct
     8581 ggacagcgaa gcgaatctga tggctcgtgt acgaacattg ctggctgaag tgcgtgacca
     8641 ggtgactcat aaaacctgtc tcaactatgt tctggaaagt ccgtactgga acgtaaaagg
     8701 taactttttc tgttatctga acgatcataa cgagaacacc atcgttgacc cttcggtgat
     8761 ctattttgat ttcgctaacc cgctgcaggc tcaggaggtc tgaatgtctg gggcaaacat
     8821 tgttcacagc ggatatggac tgcgctgtga aaaactcgac aagcctctga atcttggctg
     8881 ggggctggac aatagcgcgg tgttgcactg gcccggggag ctgccaacag ggtggctgtg
     8941 cgacgcgctg gatcagatat ttatcgccgc accacaactt tcagcagtgg ttcttccctg
     9001 gtccgaatgg tgtgaggagc cacaggcgct gacgcttttc ggacaggtac aaagcgacat
     9061 tatccatcgt tccgctttct ggcagttacc gttatggctg agttctccgg caaaccgggc
     9121 ctccggtaaa atggtttttg atgcagagcg tgagatttat ttcccgcagc gccccccccg
     9181 tccgcagggt gaagtttatc gtcgttacga tccacgcatt cgcaggatgc tgagtttccg
     9241 cattgccgat cccgtttctg atgcagaacg tttcactcgc tggatgaacg atccgcgcgt
     9301 tgagtatttc tgggagcaaa gtggctcact ggaggtacag atcgcctatc tggaacgcca
     9361 gctcaccagt aaacatgcat tcccgctgat tggttgtttc gacgatcggc cgttcagtta
     9421 tttcgaaatc tactgggcgg cggaagaccg cattggtcgc cactattcgt ggcagccctt
     9481 tgaccgtggc ctgcatctgc tggttggtga acagcaatgg cgcggagccc actatgtgca
     9541 aagctggctg cgcggggtga cacattacct gctgctgaat gagccacgta cgcagcgcac
     9601 agtactggag ccacgcaccg ataaccagcg cctgttccgc catcttgagc ctgcgggata
     9661 ccggacaatt aaagagttcg acttcccaca taaacgctcg cgcatggtga tggcggatcg
     9721 tcatcacttc ttcacggagg tcggtctgta atgaatcaca aagactggga tttggtcaac
     9781 cgccgcctgg tggcaaaaat gttgtctgag ctggagtatg agcaggtttt ccacgcggaa
     9841 tctcagggcg atgaccgcta ctgcattaac ctgccgggag cacaatggcg cttcatcgct
     9901 gaacgtggta tctggggctg gctctggatt gatgctcaaa ctctgcgctg cgcggacgag
     9961 ccagtactgg ctcagacgct gctgatgcag ctaaagcagg tactgtcaat gagcgatgca
    10021 accgttgctg agcatatgca ggatttgtat gccacgctgc tgggcgacct gcaactactg
    10081 aaagcccgtc gcgggctgag cgccagtgac ctgattaatc ttaatgccga ccgcctgcaa
    10141 tgcctgctga gcggtcatcc taaattcgtt tttaataaag gtcgccgtgg ctggggtaaa
    10201 gaggcgctgg aacgatatgc gccagagtat gccaacacct tcagactgca ctggctggcg
    10261 gtaaaacgtg aacatatgat ctggcgctgt gataacgaga tggatattca tcagttgttg
    10321 acggccgcaa tggatccgca ggagtttgcc cgcttcagtc aggtctggca ggaaaacgga
    10381 ctggatcata actggctgcc gctgccggta catccgtggc agtggcagca aaaaatcgct
    10441 accgacttca tcgctgattt tgccgaaggc aggatggtgt ctctcggcga gtttggcgac
    10501 cagtggctcg cccagcagtc gctgcgtacc ctgaccaacg ccagtcgccg gggcgggctg
    10561 gatatcaagc tgccgctgac catctacaac acctcatgct accgggggat ccctggcaga
    10621 tacatcgctg ccggaccact ggcttcacgc tggctacaac aggtttttgc gaccgacgcc
    10681 accctagtgc aaagcggcgc agtgatactt ggtgaaccgg ctgcaggcta tgtctcccat
    10741 gaaggctatg ccgcgcttgc ccgggctccc tatcgctacc aggaaatgct tggtgttatc
    10801 tggcgggaga atccgtgccg ctggctgaaa ccggatgaaa gtccggttct gatggcaaca
    10861 ctgatggagt gcgacgaaaa caatcagccg ctggcaggcg catatataga ccgctccggg
    10921 ctggacgctg aaacctggct tacgcaactg ttccgggtgg tcgtggttcc tctgtatcac
    10981 ctgctttgcc gctacggtgt cgcgcttatt gcacatggac aaaatataac tctcgccatg
    11041 aaagaggggg ttccacagcg tgttctgctg aaagacttcc agggcgatat gcgactggtg
    11101 aaagaagagt tccccgaaat ggactctttg cctcaggagg ttcgtgatgt tacatcccgc
    11161 ctgagtgcgg actacttaat ccatgatttg cagacgggtc acttcgtgac agtactgcgt
    11221 tttatttcgc cactgatggt tcgtcttggc gtacctgaaa ggcgatttta tcaactgctg
    11281 gcagcagtgt tgagtgatta catgaaaaaa catccacaaa tgtcagagcg ttttgcgctt
    11341 ttctcacttt tcaggccaca aatcattcgc gtggtgctga acccggtaaa actgacctgg
    11401 cctgatctgg atggcggcag ccgcatgctg ccgaattacc ttgaggatct gcaaaatccg
    11461 ctgtggctgg taactcagga atatgaatca tgaaaaaaag tgtcgatttt attggtgtag
    11521 ggacagggcc atttaatctc agcattgctg cgttgtcaca tcagatcgaa gaactggact
    11581 gtctcttctt tgatgaacat cctcattttt cctggcatcc gggtatgctg gtaccggatt
    11641 gtcatatgca gaccgtcttt ctgaaagatc tggtcagtgc tgttgcacct acaaatccct
    11701 acagttttgt taactatctg gtgaagcaca aaaagttcta tcgcttcctt acaagcagac
    11761 tacgtacagt atcccgtgaa gagttttctg actacctccg ctgggctgct gaagatatga
    11821 ataacctgta tttcagtcat accgttgaaa acattgattt cgataaaaaa cgtcgattgt
    11881 ttctggtgca aaccagccag ggagaatatt ttgcccgcaa tatctgcctt ggtacaggaa
    11941 aacaacctta tttaccaccc tgtgtgaagc atatgacaca atcctgtttc catgccagtg
    12001 aaatgaatct tcgtcggccg gaccttagtg gaaaacggat aaccgtggtt ggtggaggac
    12061 agagtggtgc agacctgttc cttaatgcat tacgcgggga atggggagaa gcggcggaaa
    12121 taaactgggt ctcccggcgt aataatttta acgcactgga tgaggctgct tttgctgatg
    12181 agtattttac acctgaatat atttcaggct tctccggact ggaggaagat attcgccatc
    12241 agttactgga tgagcagaaa atgacatcgg atggcatcac tgccgattct ttactgacca
    12301 tttatcgtga gttgtaccac cgttttgaag ttctgagaaa accaagaaat atccgtctgc
    12361 tacccagccg ctcggtaaca actctggaaa gtagtggtcc tggctggaag ttgctgatgg
    12421 agcatcatct ggatcagggc agggagagcc tggaaagtga tgtggtgatt ttcgccacag
    12481 gttaccgttc tgcgttgcca caaatacttc cctcactgat gcccctgatc accatgcacg
    12541 ataagaacac ctttaaagtg cgtgatgact tcactctgga atggagtggc ccgaaagaga
    12601 acaacatctt cgtggtcaac gccagtatgc aaacccatgg catcgccgaa ccccagctca
    12661 gcctgatggc atggagatct gcacgtattc ttaatcgcgt aatgggacgt gatttattcg
    12721 atctcagtat gccgcccgcc ctgattcagt ggcgcagcgg cacctaggga aaacgcagcc
    12781 ggaggctgct tctttaactc gctacacagc atctttgggc tgattttttc cgcccgtatg
    12841 gaggaataat gatgataagc aaaaagtata cgctttgggc tctcaaccca ctgcttctta
    12901 ccatgatggc gccagcagtc gctcaacaaa ccgatgatga aacgttcgtg gtgtctgcca
    12961 accgcagcaa tcgcaccgta gcggagatgg cgcaaaccac ctgggttatc gaaaacgccg
    13021 aactggaaca gcagattcag ggcggcaaag agcttaaaga cgcactggct cagctgatcc
    13081 ctggccttga cgtcagcagc cggagccgca ccaactacgg tatgaatgtg cgtggccgcc
    13141 cgctggtcgt gctggttgac ggcgtgcgtc tcaactcttc acgtaccgac agccgacaac
    13201 tggactctat agatcctttt aatatcgacc atattgaagt gatctccggt gcgacgtccc
    13261 tgtacggcgg cggcagtacc ggtggcctga tcaacatcgt gaccaaaaaa ggccagccgg
    13321 aaaccatgat ggagtttgag gctggcacca aaagtggctt tagcagcagt aaagatcacg
    13381 atgaacgcat tgccggagct gtctccggcg gaaatgagca tatctccgga cgtctttccg
    13441 tggcatatca gaaatttggc ggctggtttg acggtaacgg cgatgccacc ttgcttgata
    13501 acacccagac cggcctgcag tactccgatc ggctggacat catgggaact ggtacgctga
    13561 acatcgatga atcccggcag cttcagttga tcacacagta ctataaaagc cagggcgacg
    13621 acgattacgg gcttaatctc gggaaaggct tctctgccat cagagggacc agcacgccat
    13681 tcgtcagtaa cgggctgaat tccgaccgta ttcccggcac tgagcggcat ttgatcagcc
    13741 tgcagtactc tgacagcgct tttctgggac aggagctggt cggtcaggtt tactaccgcg
    13801 atgagtcgtt gcgattctac ccgttcccga cggtaaatgc gaacaaacag gtgacggctt
    13861 tctcttcgtc acagcaggac accgaccagt acggcatgaa actgactctg aacagcaaac
    13921 cgatggacgg ctggcaaatc acctgggggc tggatgctga tcatgagcgc tttacctcca
    13981 accagatgtt cttcgacctg gctcaggcaa gcgcttccgg agggctgaac aacaagaaga
    14041 tttacaccac cgggcgctat ccgtcgtatg acatcaccaa cctggcggtc ttcctgcaat
    14101 caggctatga catcaataat ctctttaccc tcaacggtgg cgtacgctat cagtacactg
    14161 aaaacaagat tgatgatttc atcggctacg cgcagcaacg gcagattgcc gccgggaagg
    14221 ctacatccgc cgacgccatt cctggcggct cagtcgatta cgacaacttc ctgttcaacg
    14281 ccggtctgct gatgcacatc accgaacgcc agcaggcatg gctcaacttc tcccagggcg
    14341 tggagctgcc ggacccgggt aaatactatg gtcgcggcat ctatggtgct gcagtgaacg
    14401 gccatcttcc tctaacaaag agtgtgaacg tcagcgacag caagctggaa ggcgtgaaag
    14461 tcgattctta tgagctgggc tggcgcttta ctggcaataa tctgcgtacc caaatcgcgg
    14521 cctactattc gatttctgat aagagcgtgg tggcgaataa agatctgacc atcagcgtgg
    14581 tggacgacaa acgccgtatt tacggcgtgg aaggtgcggt ggactacctg attcctgata
    14641 ctgactggag taccggagtg aacttcaacg tgctgaaaac tgagtcgaaa gtgaacggta
    14701 cctggcagaa atacgatgtg aagacagcaa gcccatcaaa agcgacagcc tacattggct
    14761 gggcaccgga cccgtggagt ctgcgcgtgc agagcaccac ctcctttgac gtgagcgacg
    14821 cgcagggcta caaggtcgat ggctatacca ccgtggatct gctcggcagt tatcagcttc
    14881 cggtgggtac actcagcttc agcattgaaa acctcttcga ccgtgactac accactgtct
    14941 gggggcagcg tgcaccactg tactacagcc cgggttacgg cccagcgtca ctgtacgact
    15001 acaaaggcag gggccgaacc tttggtctga actactctgt gctgttctga ccggtattcc
    15061 tttacaacaa aggtacgctg atatcaacat ggccgctgac agccaagttg atatcatata
    15121 atacacgaca taatctgtag tcagggagga tagactcttt actgactaca gattatgtcg
    15181 tgttccgtgc tcatttcctc aaaaaaatac aagaaaagaa ttagtattct aacaaaaagt
    15241 gaaataaatt gtatcaaact ccctctttta atcctgttga gtaaatcagc ttttgcaata
    15301 ggattgaaag agtgtaagtg gaatctcttc cggatactcg ttaccaccgt ggctagaata
    15361 tctacggctg cgggccagta atagattaag tgataacagc tgtctggaaa tgtaggggcg
    15421 tatccactaa tcataatcat tgtgagataa atattacggg gcattttata atgaataa
//
LOCUS       DAMZET010000097        15313 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.097, whole genome shotgun sequence.
ACCESSION   DAMZET010000097 DAMZET010000000
VERSION     DAMZET010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.097"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            88..1887
                     /gene="cysJ"
                     /locus_tag="POB34_003076"
     CDS             88..1887
                     /gene="cysJ"
                     /locus_tag="POB34_003076"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HDI5919973.1"
                     /translation="MTTQVPPSALLPLNPEQLVRLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            1887..3599
                     /gene="cysI"
                     /locus_tag="POB34_003077"
     CDS             1887..3599
                     /gene="cysI"
                     /locus_tag="POB34_003077"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HDI5919974.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            3673..4407
                     /gene="cysH"
                     /locus_tag="POB34_003078"
     CDS             3673..4407
                     /gene="cysH"
                     /locus_tag="POB34_003078"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDI5919975.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMLEEETRFFGLKRECGLHEG"
     gene            4672..4824
                     /locus_tag="POB34_003079"
     CDS             4672..4824
                     /locus_tag="POB34_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDI5919976.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            5018..7717
                     /locus_tag="POB34_003080"
     CDS             5018..7717
                     /locus_tag="POB34_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDI5919977.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKEGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     ELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGRPPDRMDELDNFLPEDKAAARDFLLAIKALFPRIEIPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAQAMDIKHYWQKALVQAQNALTVFPPKAETAPFTGI
                     NTLFPFIENPTPLQQKVLDLDISQPGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGAEGQ
                     QEAPLLGKDDYPWLTHVTKTDVHSHRVATRKEVERSVSVGWLHSEQECIARIESAVSQ
                     GKCIAWIRNSVDDAIKVYRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKE
                     DGSQRAGKVLICTQVLEQSVDCDLDEMISDLAPIDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQM
                     PHSARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRARAKKFVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGDAFRQWCIEQRQDPEMANVILVTDDESCGYSAMEGLTGKVG"
     gene            7815..9377
                     /gene="casA"
                     /locus_tag="POB34_003081"
     CDS             7815..9377
                     /gene="casA"
                     /locus_tag="POB34_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HDI5919978.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDHRRWDDIWEDGLEVEKLREALLSLEHAFQFGPDSPSFM
                     QDFEALTGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEYLCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKMWLNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTDDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQNDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRFVRQIE
                     EGQDADELLSKWNKEIWLFARQDFDERVFTNPYEPVNLERIMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            9374..9910
                     /gene="casB"
                     /locus_tag="POB34_003082"
     CDS             9374..9910
                     /gene="casB"
                     /locus_tag="POB34_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HDI5919979.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            9922..10977
                     /gene="cas7e"
                     /locus_tag="POB34_003083"
     CDS             9922..10977
                     /gene="cas7e"
                     /locus_tag="POB34_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HDI5919980.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWAMAEKGTEQPRSLAAAFYEPINGTRQLEVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            10988..11734
                     /gene="cas5e"
                     /locus_tag="POB34_003084"
     CDS             10988..11734
                     /gene="cas5e"
                     /locus_tag="POB34_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HDI5919981.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTIQMPKEVRKARYFSRREELSDPD
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTINQGPWLSKEEPCTSQE"
     gene            11716..12366
                     /gene="cas6e"
                     /locus_tag="POB34_003085"
     CDS             11716..12366
                     /gene="cas6e"
                     /locus_tag="POB34_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HDI5919982.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELRTGQQLCFNLRANPTICKS
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            12363..13286
                     /gene="cas1e"
                     /locus_tag="POB34_003086"
     CDS             12363..13286
                     /gene="cas1e"
                     /locus_tag="POB34_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HDI5919983.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAVVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            13283..13576
                     /gene="cas2"
                     /locus_tag="POB34_003087"
     CDS             13283..13576
                     /gene="cas2"
                     /locus_tag="POB34_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HDI5919984.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     repeat_region   13673..14250
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=13673..13701
                     /rpt_unit_seq="gtgttccccgcgccagcggggataaaccg"
     gene            complement(14332..>15313)
                     /gene="iap"
                     /locus_tag="POB34_003088"
     CDS             complement(14332..>15313)
                     /gene="iap"
                     /locus_tag="POB34_003088"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HDI5919985.1"
                     /translation="LPVHASSPKPGDFANTQARHIATFFPGRMTGTPAEMLSADYIRQ
                     QFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIAAHEGKAPQQIIIMAHLDTY
                     APLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKNTPTEYGIRFVATSGEEEGK
                     LGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGVKTPEAVRKLTRDRALAIAR
                     SHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSVEATNWNLGNKDGYQQRAKT
                     AAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIMLPLVKELAKAS"
BASE COUNT         3763 a         3779 c         4191 g         3580 t
ORIGIN      
        1 ttcaccccgt taaccttacc ttctcttctg ttttatgggc gctgacaggg cgcagaaaca
       61 gctttgctta ctggaacata acgacgcatg acgacacagg tcccaccttc cgcgttgctt
      121 ccgttgaacc cggagcaact ggtacgcctt caggcggcca cgaccgattt aactcccacc
      181 cagcttgcct gggtttctgg ctatttctgg ggcgtgctca atcagcagcc tgctgcgctt
      241 gcagcgacgc cagctcccgc cgcagaaatg ccgggtataa ctattatctc cgcctcgcaa
      301 accggcaatg cgcgccgtgt tgctgaagca ttacgtgatg atttattagc ggcaaaactg
      361 aacgttaagc tggtgaacgc gggcgactat aaattcaaac aaatcgccag cgaaaaactg
      421 cttatcgtgg tgacgtcaac gcaaggagaa ggggaaccgc cggaagaagc cgtcgcgctg
      481 cataagttcc tgttctccaa aaaagcgccg aagctggaaa acaccgcatt tgccgtgttt
      541 agcctcggcg atagctctta tgaatttttc tgccagtccg ggaaagattt tgacagcaag
      601 ctggcggaac tgggtggtga acgcctgctc gaccgtgtcg atgccgatgt tgaataccag
      661 gctgctgcca gcgagtggcg cgcccgcgtg gttgatgcgc ttaaatcgcg tgcgcctgtc
      721 gcggcacctt cgcaatccgt cgctactggc gcggtaaatg aaatccacac cagcccgtac
      781 agcaaagacg cgccgctggt ggctagcctc tctgttaacc agaaaattac cgggcgtaac
      841 tctgaaaaag acgttcgcca tatcgaaatt gacttaggtg actcgggcct gcgttaccag
      901 ccgggtgacg cgctgggcgt ctggtatcag aacgatccgg cactggtgaa agaacttgtc
      961 gaactgctgt ggctgaaagg cgatgaacct gtcaccgtcg agggcaaaac gttgcctctg
     1021 aacgaagcgc tacagtggca cttcgaactg accgtcaaca ccgccaacat tgttgagaat
     1081 tacgccacgc ttacccgcag cgaaacactg ctgccgctgg tgggcgataa agcgaagtta
     1141 cagcattacg ccgcgacgac gccgattgtc gacatggtgc gtttctcccc ggcacagctt
     1201 gatgccgaag cgctaattaa tctgctgcgc ccgctgacgc cgcgtctcta ttccattgcc
     1261 tcctcgcagg cggaagtcga gaacgaagta cacgtcaccg ttggtgtggt gcgttacgac
     1321 gtggaaggcc gcgcccgtgc cggtggtgcc tccagcttcc tcgctgaccg cgtggaagaa
     1381 gagggcgaag tccgcgtatt tatcgaacat aacgataact tccgcctacc agccaatcca
     1441 gaaaccccgg tgattatgat tggcccaggc accggtattg cgccgttccg cgcctttatg
     1501 cagcaacgcg ccgccgacga agcgccaggt aaaaactggc tgttctttgg taatccgcac
     1561 tttacggaag acttcctgta ccaggtggag tggcagcgct acgtcaaaga tggcgtgctg
     1621 acacgtatcg atcttgcctg gtcgcgcgac caaaaagaaa aagtttacgt acaagacaaa
     1681 ctgcgcgaac agggcgcgga gctgtggcgc tggatcaatg atggtgccca catttatgtc
     1741 tgcggcgacg ctaatcgcat ggcgaaagac gttgagcagg cacttctgga agtgattgcc
     1801 gaatttggtg gcatggacac cgaagcggcg gatgaatttt taagtgagct gcgcgtagag
     1861 cgccgttatc agcgagatgt ctactaatga gcgaaaaaca tccagggcct ttagtggtcg
     1921 aaggaaaact gacagacgcc gagcgcatga agcttgaaag caactacctg cgcggcacca
     1981 ttgcggaaga tttaaacgac ggtctgaccg gcggctttaa gggcgataac ttcctgctga
     2041 tccgcttcca cggcatgtat cagcaggatg accgcgacat ccgcgccgaa cgtgctgaac
     2101 agaagctgga gccgcgccac gcgatgctgc ttcgctgtcg tctgccgggt ggggtgatta
     2161 ccactaaaca gtggcaggcg atcgacaaat ttgccggtga aaacaccatc tatggcagca
     2221 ttcgcctgac caaccgccag acgtttcagt tccacggcat tctgaaaaag aacgtcaaac
     2281 cggtgcacca gatgctgcac tcggtcggtc ttgatgcgct ggcgacagct aacgacatga
     2341 accgtaacgt actctgcacc tcgaaccctt acgagtcgca gctgcacgcg gaagcgtacg
     2401 agtgggcgaa gaagatttct gagcatctgt tgcctcgtac ccgcgcgtat gcggagatct
     2461 ggctcgacca ggaaaaagtc gccactactg atgaagaacc gatccttggt cagacctacc
     2521 tgccgcgtaa gttcaaaacc acagtagtga tcccgccgca gaacgatatc gatctgcatg
     2581 ccaacgacat gaacttcgtg gcaatcgccg aaaacggcaa gctggtaggc ttcaacctgc
     2641 tggtgggcgg tgggctttct atcgaacacg gcaacaagaa aacctacgcc cgcacggcga
     2701 gcgagtttgg ctatctgccg ctggagcata cgctggcggt ggccgaagcc gtcgtgacaa
     2761 ctcagcgtga ctggggtaac cgaaccgatc gtaaaaatgc caaaaccaaa tacacgctgg
     2821 agcgcgtggg ggttgagacg tttaaagcgg aagtggagcg tcgcgcgggg atcaaatttg
     2881 aaccgatccg tccatatgag ttcaccggac gaggcgatcg tattggctgg gttaagggca
     2941 ttgatgataa ctggcacctg acgctgttta tcgaaaatgg tcgcatcctt gattatccgg
     3001 ggcgtccgct gaaaaccggc ctgctggaga tcgcgaagat ccacaaaggc gatttccgca
     3061 ttacggcgaa ccagaatctg atcatcgccg gtgtaccgga aagcgagaaa gcgaagatcg
     3121 agaagatcgc caaagagagc ggtttaatga atgccgtcac gccgcagcgt gaaaactcga
     3181 tggcctgcgt gtcattcccg acttgcccgc tggcgatggc ggaagcggag cgtttcctgc
     3241 cgtcttttat cgacaacatc gataatttaa tggcgaaaca tggtgtcagc gatgagcata
     3301 tcgtgatgcg tgtaacaggc tgcccgaacg gttgtggtcg cgcgatgctg gcggaagtgg
     3361 gcctggtggg taaagcaccg ggtcgctaca atctgcatct tggcggcaac cgcattggga
     3421 cacgtatccc acggatgtat aaagaaaaca tcaccgagcc ggaaatcctg gcgtcgcttg
     3481 atgaactgat agggcgctgg gcgaaagagc gcgaagcggg tgaaggcttc ggcgacttta
     3541 cggtgcgtgc gggcatcatt cgcccggtgc tcgatccggc gcgcgatttg tgggattaac
     3601 catcagcccg gtcttgtagg cctgataaga cgcgtgagcg tcgcatcagg caaggcaaac
     3661 agtgaggaat ctatgtccaa actcgatcta aacgccctga acgaactgcc gaaggtagat
     3721 cgcattctgg cgctggcaga aactaacgcc gaactggaaa aactggacgc tgaaggccgc
     3781 gttgcctggg cgctggataa tctgcccggt gaatatgtac tttcttccag ctttggcatt
     3841 caggcggcgg tgagcctgca tctggtgaat cagattcacc cggatattcc ggtgatcctc
     3901 accgacaccg gctatctgtt cccggaaacc taccgcttta ttgacgagtt aacggacaaa
     3961 ctcaagctca acctgaaagt gtaccgcgct accgaaagcg ccgcctggca ggaagcacgc
     4021 tacggcaaac tgtgggaaca gggcgttgaa ggcattgaaa agtacaatga catcaacaaa
     4081 gtcgaaccga tgaaccgggc gctgaaagaa ctgaatgcgc aaacgtggtt tgctggcctg
     4141 cgccgcgaac aatccggcag ccgcgccaat ttaccggtgc tggcaattca gcgtggcgta
     4201 tttaaagtgc tgccgattat cgactgggat aaccgaacta tttatcagta cctgcaaaaa
     4261 catggcctga aatatcaccc attatgggat gaaggatatt tatcggtcgg tgatacccat
     4321 acaacccgta aatgggaacc tggcatgttg gaagaagaaa cacgtttctt tggcttaaaa
     4381 agggaatgtg gattgcacga agggtaaata ttgccggata aattaaacgc ctctgtcaga
     4441 aatgatgggg gcgtttttat tttgcgagca gtattcctga ttttaaacgt gaacgccgct
     4501 ggaagaatta tcaggtatag aatggcgtcg ggtgcttgag gctgtctggc gttaagcatt
     4561 cgcgaggttc cagatggaca aaagccccag gcgatatttc tatcaacctg aggcttacgt
     4621 tcgaacccaa acaattcgaa tgttagtctc ttctttgcag ggaggcaaga catgctgaca
     4681 aaatatgccc ttgtggcaat catcgtactg tgttgtacag tactgggatt cacgctgatg
     4741 gtaggtgact cgttgtgtga gttgagtatc agagaacgtg gtatggagtt taaggcagtt
     4801 ctcgcttacg aatcgaagaa gtagccacca cgcgggggta aaaccccgcg tactgaattg
     4861 tgatggtttg gtctcctgca ccctttttta aaatttatat tttttatgtg aatatatagt
     4921 ctctatttat tattatgaat ttcaaatgaa aatcattaat ttattcataa tgtaaatgtt
     4981 ttattggctg atatatatca cagataattt ttaaaatatg cgtaaatatc ctttaagttt
     5041 actgaaggat aaaaatattg tgactttctt tgatttctgg ggaaaaaccc gacgcggcga
     5101 gaaagaaggg ggcgacgact atcaccttct ttgctggcat tcgctggatg tggccgcgat
     5161 gggctattta atggttaaaa gaaattgctt cgggctggct gattattttc gtcaattagg
     5221 gatttctgac aaggaacagg cggctcaatt tttcgcctgg ttgctgtgct ggcacgatat
     5281 tggaaaattt gcccgctctt ttcaacaact ttacctgccc cctgaactca agattcagga
     5341 aggtgccaga aaaaattacg aaaaaatctc tcattcaacg ctgggctact ggttgtggaa
     5401 tcattattta agtgaatgtc aggagttact tccttcatct tcgctctctc cgcgcaaact
     5461 taggcgagtg atagaaatgt ggatgcccgt gacgacaggg catcatggtc ggccccctga
     5521 tcgtatggat gaattggata attttctgcc tgaagacaaa gctgcagcac gagattttct
     5581 ccttgcaatc aaggcactgt ttccgcgaat agagatcccc gcattctggg atgatgacga
     5641 gggcattgaa cttataaaac acctttcctg gtatatctct gcaaccgtcg tactcgcaga
     5701 ctggacaggt tcgtcaacgc gattttttcc acgcgtcgca caggcaatgg atattaaaca
     5761 ttactggcag aaggctttag tgcaggctca aaacgcatta accgtctttc ctccaaaagc
     5821 agaaaccgca cctttcaccg gaattaatac actgtttcct tttattgaga atccgacacc
     5881 gttacagcaa aaggtgctgg atctggatat cagccagcca ggaccgcagt tatttattct
     5941 ggaagatgtg actggcgcag gtaaaacaga agcggcgctt atcctggcgc acaggttgat
     6001 agctgcgggg aaagctcagg ggctgttttt tggtctgcca acaatggcaa cggccaatgc
     6061 catgtacgat cggctggtca aaacctggct tgctttctat tcgccagaat cccgccccag
     6121 cctggtgctg gcacatagtg cccgcacatt aatggaccgc tttaatgaat cactctggtc
     6181 cggtgattta gtcgggtcag aagaaccgga tgaacaaacg ttcagtcagg gatgtgcggc
     6241 ctggtttgcc gacagtaaca agaaggcgtt gctggctgaa attggcgtcg gcacgctgga
     6301 tcaggcgatg atggcggtga tgccgttcaa acataataat ctgcggcttc tggggctgag
     6361 taacaaaatc ctgctggctg atgagatcca cgcctgtgac gcttacatgt cgtgcattct
     6421 tgaagggctg atcgagcggc aggcgcgtgg cggaaacagc gttattttgc tttctgctac
     6481 gttatctcaa cagcagcgcg acaaactcgt cgccgccttt gcgcgtggtg cagagggcca
     6541 gcaagaagcc ccactcctgg gaaaagatga ttacccctgg ctgacgcatg tcacgaaaac
     6601 cgatgtacat tcgcaccggg tagcgacgcg caaagaagtt gagcgtagcg tgagcgtggg
     6661 ttggcttcat agcgaacaag aatgtattgc gcgtatcgaa tcagcggtaa gtcaggggaa
     6721 atgcatcgcc tggatccgga attctgtcga tgacgctatt aaggtttatc gtcagttact
     6781 tgcccgcggc gtcattcctg cttccagcct ttcacttttt catagccgct ttgcttttag
     6841 cgatcgccag cgaattgaaa cggaaacgct ggcacgcttt ggtaaagaag acggttcaca
     6901 gcgtgccgga aaagtcctca tttgtactca ggtcttagag cagagcgttg attgtgacct
     6961 ggacgaaatg atctccgacc tggcccccat tgatttgcta atccagcgag caggacgatt
     7021 acagcggcat atccgcgata ttaatggtca gttaaagcgt gacggaaaag acgagcgttc
     7081 cccccctgaa ttgctgattc tggctcccgt ctgggacgac tctcctggcg acgaatggtt
     7141 tggcagtgcc atgcgtaaca gtgcatttgt ctatcccgat catgggcgaa tctggctgac
     7201 gcagcgtgtg ctgcgtgagc aaggtgctat tcagatgcca cactcagccc ggcttcttat
     7261 tgaatccgtc tacggtgagg acgtggtaat gccggaagga tttgcccgca gcgagcagga
     7321 gcaagtgggc aaatattact gtgatcgcgc aagggctaaa aagtttgtgc tgaacttcag
     7381 gcccggctat gccgccaata tcaacgatta cctcccggaa aaactgtcga cacgtctggc
     7441 tgaggagtct gtttccctgt ggctggctac ctgtattgac ggtgtggtga agccttatgc
     7501 caccggtgct cacgcatggg aaatgagcgt tgtcagagtg cgccgaagct ggtggaaaaa
     7561 acatcgggat gagttttctt tactggaagg ggatgcgttc aggcagtggt gcattgaaca
     7621 gcggcaagat ccggagatgg caaacgtgat tttagtcacc gatgacgaaa gttgcgggta
     7681 ttcggctatg gaaggattga ctggcaaggt tggttgagat gcctgcgaca gggctctctg
     7741 aatagttgat acaccgaaaa ggatggaatg atggacaacc gacctgggat ggtctttttt
     7801 acgagaggat gcgtatgaac tcgttttcac ttctgacaac cccgtggttg cctgttcgtt
     7861 tcaaagatgg aacgacaggc aaactggcgc ctgtcgatct ggcggatgaa aatgttgtcg
     7921 atattgctgc gccacgggca gatctccagg gagcggcatg gcagtttttg ctggggctac
     7981 tacaaaccag ctttgcgcca aaagatcatc gtcgttggga tgatatctgg gaagacggac
     8041 tggaagttga aaagctacgg gaagcattgc tgtccttaga acacgctttc cagtttgggc
     8101 cagattcccc ttcatttatg caggattttg aggcgctcac gggagataaa gttcaggtcg
     8161 cttcactact gcctgagatt cccggcgctc aaacaacgaa gtttaataaa gaccacttta
     8221 tcaagcgtgg tgtaacggaa tacctgtgcc cacattgttc tgcgttagct ctgttctccc
     8281 tacagttaaa tgcgccgtca ggtggtaaag gctaccgcac cggcttacgc ggcggtgggc
     8341 cgatgaccac gctgattgaa ttacaggaat atcagggcaa tcaacaaact cccttgtggc
     8401 gcaaaatgtg gctcaacgtg atgccgcagg atgaagccga tttaccgcta cccaaaaaat
     8461 ttgacgatct ggttttcccc tggcttggcc cgacgcgcac cagcgaactg gccggtgcgg
     8521 tggtaaccga tgatcaggtt aataaactcc aggcgtactg gggaatgccg cggcgtattc
     8581 gtattgattt taataccacg acagtcggca actgcgatat ttgcggcgag cagaacgacg
     8641 cgctcctgag cctgatgacg accaaaaatt acggtgcgaa ttatgccatg tggcagcatc
     8701 ccttaacgcc ttaccgtgta ccacttaaag agggtggcga gttttactcc gttaaaccac
     8761 aaccgggcgg tttaatctgg cgtgactggt taggccttat cgaaacgggt aaatcagaaa
     8821 acaatacgga acttcccgcg ctggtggtga aactctttaa tgccagcagt ctgaaacagg
     8881 caaaagtggg cctgtgggga tttggttatg atttcgacaa catgaaagcg cgctgttggt
     8941 acgaacacca tttccctcta ttgctcaata aaaaagaagg ccagatacca aagctgcggc
     9001 tggctgcgca aacggcttca cggattctaa gtctgttacg gagtgcattg aaagaagcat
     9061 ggttctctga tccaaaaggt gcaaggggtg attttagttt tgtggatatc gacttctgga
     9121 acaaaactca gcatcgcttt ctgaggttcg tgcgccaaat tgaagaaggt caggatgcgg
     9181 atgaattact cagcaaatgg aataaggaaa tttggttatt cgcgcgtcag gattttgacg
     9241 agcgtgtatt cactaatcct tatgagcccg ttaatttgga acgcatcatg accgcgcgca
     9301 agaaatattt tacaacatcg gcggagaagc aaagtgctaa agccgccagg gagaaaaagc
     9361 aggaggctgc tgaatgagta ttgtaaaaga ggagcacaaa gccacgctgc gcaaatggca
     9421 cgaggaattg caggagaaac gcggcgaacg tgccagtctg cggcgtagta cgacggttaa
     9481 tgatgtttgt ctgacggatg gatttcgcct gtttctgaag aacagacaaa taaagtggca
     9541 ggatgaacct gagtggcgta tcaccgcact agcgctgata gccgcagtta gcgcgaatgt
     9601 taaagccatt gatgaacggc agccttttgc cgcgcagctg gcggcggtaa tgtcaaaggg
     9661 gcgttttact cgcctgtcgg cagtaaaaac accggatgaa ttactgcgcc agttacggcg
     9721 cgcagtcaga ttattaaacg gttccgtcaa tcttgactct ctggcagagg gggtcttccg
     9781 ctggtgccag gagagcgacg acctgctcaa tcatcaccgc cgccagcagc ggccaaccga
     9841 atttatccgt atccgctggg cgctggaata ttaccaggct ggcgacgccg ataacgaaca
     9901 aaatcaataa aggactatct catgacaaca tttattcagc ttcatttgtt aaccgcttac
     9961 cctgctgcca accttaaccg tgatgatact ggtgcgccaa aaactgtggt actgggtggc
    10021 gcaacgcgtc tgcgcgtttc ctcacaaagt ctgaaacgtg cgtggcgcac ttctgcactt
    10081 tttgaacagg cactggcggg ccatattggt attcgtagtg ggcgtattgc gcgtgaggcg
    10141 gcaactatcc tgattgagaa aggaatcgaa gagaaaaaag ccatcgaatg ggcggcaaaa
    10201 attgcggatt atcttgggaa agctaaaaac gacaaaaaac caaaagatcc gctcactaac
    10261 gccgaaactg aacaattagt tcatatcagc ccggcagaat ttgacgccgt aaaagcgctg
    10321 gcccatcagc ttgcagaaga aaagcgcgcg ccaaaagagg aagatctcgc gttgttacgc
    10381 aaagaccgta tggcggtaga tattgccatg tttggtcgta tgctggcgaa taaacccgag
    10441 tttaatgttg aagccgcctg ccaggtagcg cacgcatttg gtgtcagtga aacgattgtc
    10501 gaagatgatt ttttcaccgc cgttgatgat ttgcgccagg cttctgaaga tgccggtgcc
    10561 gggcatcttg gtgaaaccgg attcggttct gcactgttct acacctatat ctgcattgat
    10621 aaagatctgc tggtcgaaaa cctcggcggt gacgaagcgt tagctaatca gaccatacgc
    10681 gcctttacgg aagccgcgct taaagtctcc ccaaccggca aacagaacag ctttgccagc
    10741 cgtgcctacg cctcctgggc gatggcagaa aaaggtaccg aacaaccgcg ttctctggcg
    10801 gcggctttct atgaacccat taatggcacc cggcagttag aggtggcggt gcagcgtatt
    10861 acaacgcttc gcgaaaacat gaatacggtc tacgaacaga agaccgaatg cgcaagtttt
    10921 gacgtgatga acaaacaggg aagcatgaag gacgtgctgg actttatctg cgcgtaaggg
    10981 gcggcttatg agccaatatc tgatttttca gcttcatggg ccaatggcat cctggggcgt
    11041 cgatgccccc ggcgaagtgc gtcataccca tgaactgcct tcgcgctcag cattgctggg
    11101 gctgctggct gccggggtag ggattcggcg tgatgatacc gaacgcttaa acgcgtttaa
    11161 tcgccactat tcactggtgg tttgcgccag ccgtaacccg cgctgggcac gggattatca
    11221 cactatccag atgccaaaag aggtgcgtaa agcgcgttat ttcagccgtc gcgaagagtt
    11281 gagcgaccct gatcttctga gcgcgattat ctcccggcgc gactactaca ccgatgcctg
    11341 gtggatggtg gcggtggcaa caacagccga tgcgccttac agcctcgaac agttgcagga
    11401 cggtttacgc catccagttt ttccgcttta tctggggcga aaaagtcatc cgctggcatt
    11461 acctcttgcg ccgttactgc tcgaaggcaa cgcgtgtgat gccttatgta atgcatacca
    11521 acagtatcag gatcatttcc ataaactgaa agtctcactc ccgaaacttc aggatgaatg
    11581 ctggtgggaa ggggaacacg acggcctggt cgcgagcaaa atattacgcc gtcgggatgt
    11641 tcctttaaat cgtcagcagt ggttgtttgg ggaacgcacc atcaatcagg ggccgtggct
    11701 cagcaaggag gaaccatgta cctctcaaga ataaccttac ataccggcca gctttcgcct
    11761 gcgcagttgc tgcatctggt ggatcgcggt gaatatgtga tgcatcagtg gctgtgggat
    11821 cttttccctg gcggcaaaga aaggcaattt ctttatcgtc gggaagaact ccagggcgcg
    11881 tttcgctttt ttgtcctctc gcaggaacgc ccggcggaaa gtgatacgtt caccatagaa
    11941 tgccggtcat ttgcgcccga gctgcgtacc ggacaacaac tttgtttcaa cctgcgggcg
    12001 aatccaacaa tctgtaaatc gggcaagcgc cacgatctgc tgatggaggc gaaacggcag
    12061 gtgaggggac aggcggaagg aagtgatgtc tggttgcatc aacaacaggc ggcgctggac
    12121 tggctggcag cgcaggggga aagaagtggt tttacactac tggatacctc ggttgatgcc
    12181 taccgacaac agcaactccg gcgggaaaac tcccggcaac tgatccagtt cagtagtgtg
    12241 gattatacgg ggatgcttac ggtcaccgat cccgggttat ttctgcaacg tcttagccag
    12301 ggatatggca aaagccgggc gttcggctgt ggtctgatgc tgatcaaacc cggagcagag
    12361 gcgtgacgtt tgtaccactg agtccgatcc cgttaaaaga tcgcacctcc atgatcttcc
    12421 tccagtacgg tcagatcgac gtactggacg gcgctttcgt gctgatcgac aaaaccggga
    12481 tccgcacgca cattccggtg ggatcggtcg cctgcattat gctcgaaccg ggaacgagag
    12541 tttcccacgc ggcggtgcat ctagccgcca cggtggggac gctactggtc tgggtcggtg
    12601 aagcggtcgt tcgcgtttac tcctccggac aacccggagg ggcgcgggca gataaattac
    12661 tctaccaggc aaagctggct ttaacggaag atctacggtt gaaggtggtg cgcaaaatgt
    12721 atgaattacg ttttcgtgaa ccaccgccag ctcgccgttc agtggagcag ttacgtggaa
    12781 ttgagggatc tcgcgttcgc cagacgtatg cattactggc gaaacaatat ggtgtgaaat
    12841 ggaatggtcg caaatacgat cctaaagact gggaaaaagg cgatgttgtg aatcgctgca
    12901 tcagtgctgc tacatcatgt ctgtacggta tatctgaagc ggcagtatta gccgcgggat
    12961 atgcgcccgc tattgggttt atccatagtg gcaaaccgct ttcatttgtt tatgacatag
    13021 ccgatatcat taaatttgat tcggttgtgc caaaggcatt tgaaatagca gcgaggcaac
    13081 ccgcagaacc tgataaagaa gtcagattag cctgtcgcga tattttccgt agcactaagt
    13141 taacgggcaa attaataccg ttaattgagg aagtccttgc tgcaggtgaa attgaaccac
    13201 cacaacccgc gccggatatg ttaccgcctg ccatccctga acctgaaacg ctgggtgata
    13261 gtggtcaccg ggggcgcggc ggatgagcat ggtcgtggtt gttacagaaa atgtccctcc
    13321 gcgcttacgt ggacggctcg caatctggct actggaagtg cgtgccggtg tgtatgttgg
    13381 tgatacatca aaacgtattc gggagatgat ctggcagcaa attacccaac tggctggttg
    13441 cggaaatgtg gtgatggcct gggcgaccaa taccgagtcg ggttttgaat ttcagacctg
    13501 gggagaaaac agacgtattc cggtggattt ggatgggtta cgtttggttt cttttcttcc
    13561 tgttgataat caataagtta gacgttcttt aaaaataagg aaatgtttta atttagttgg
    13621 tagattgttg atgcggaata aatttgttta aaaacagtta tgtatgctta gtgtgttccc
    13681 cgcgccagcg gggataaacc gttgcccgcg caattccggg agcatccgca attgtgttcc
    13741 ccgcgccagc ggggataaac cgacggacaa aatatatatt gatttgcgaa ttatgtgttc
    13801 cccgcgccag cggggataaa ccggtaaaga aactgccgac aaatccctgt tcgttgtgtt
    13861 ccccgcgcca gcggggataa accgcccgtc accgacgcgc agtggcgcta ccgtgagtgt
    13921 tccccgcgcc agcggggata aaccgggatc taacgcgctg taaaaattcc gtgctttgtg
    13981 ttccccgcgc cagcggggat aaaccatgcg gattaccggc aaaacatggg agcaaaccgt
    14041 gttccccgcg ccagcgggga taaaccgccg aacggctggc gaagcaggtg gctggcgtag
    14101 tgttccccgc gccagcgggg ataaaccggt ttaccgcccc gcagaggcgc tggcagatcc
    14161 gtgttccccg cgccagcggg gataaaccgg gatgacctgt cgctaaaact cgccgcgtac
    14221 agtgttcccc gcgccagcgg ggataaaccg ggcgcactgg atgcgatgat ggatatcact
    14281 tagaattccc cgcccctgcg gtagaacacc cagctcccat tttcaaaccc atcaagacgc
    14341 cttcgccaac tccttcacca gaggtagcat tatccgcata acgtcacggc agcgacgttc
    14401 tattcttcca ggaagtgcct tatcaatatg ctgttgatta tccagtctta cgtcatgcca
    14461 gctatttccc gcagggaatg cggctgtttt tgcgcgttgc tgataaccat ccttattccc
    14521 aagattccag ttagtcgctt ccaccgaaag tacagcaatg cccgctttgt cgaatatttc
    14581 tgcgtcatta caacacccag tgcctttcgg ataattttta ttcaaacccg gattggtcgt
    14641 tgcggctatt ccgtgactgc gtgcaattgc cagcgccctg tcgcgcgtta atttccttac
    14701 tgcttcaggg gtttttacac cgctgttgaa atacaattta tcgccaacaa ttaagttatc
    14761 gagattaatc accagcagcg tatttttctt ttcggtgtca ctcatccgct tgagtaaatt
    14821 ctcagcgcct aatttccctt cctcttcgcc gctggtcgcc acaaatcgaa taccatactc
    14881 ggtaggcgta tttttcaggc gttctgccaa ttccagcatg acacctaaac ctgcggcgtt
    14941 atcatccatt ccttgtaacg tcagcccgcc gagattggca tcggcgtcag catcgctcag
    15001 cggggcgtaa gtatccagat gcgccataat gatgatctgc tgcggcgctt tgccttcatg
    15061 agcggcaatc accgtacttc ccgtcacgtt atgccagctc ttacgattat cgcgggcggt
    15121 ataaatatac cgactattaa atgtccgaat atcactgcga taacccattt gctgaaactg
    15181 ttggcgaata taatcggcag ataacatttc tgcaggagtt ccggtcatgc gtcccggaaa
    15241 gaaagtagca atatgtcgtg cctgagtatt agcaaaatcg ccaggtttag gtgacgaggc
    15301 gtgtacgggg aga
//
LOCUS       DAMZET010000098        15283 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.098, whole genome shotgun sequence.
ACCESSION   DAMZET010000098 DAMZET010000000
VERSION     DAMZET010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.098"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            416..1564
                     /gene="cfa"
                     /locus_tag="POB34_003089"
     CDS             416..1564
                     /gene="cfa"
                     /locus_tag="POB34_003089"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HDI5919986.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIASAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(1604..2245)
                     /gene="ribE"
                     /locus_tag="POB34_003090"
     CDS             complement(1604..2245)
                     /gene="ribE"
                     /locus_tag="POB34_003090"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDI5919987.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            2460..3833
                     /gene="mdtK"
                     /locus_tag="POB34_003091"
     CDS             2460..3833
                     /gene="mdtK"
                     /locus_tag="POB34_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HDI5919988.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            complement(3874..5130)
                     /locus_tag="POB34_003092"
     CDS             complement(3874..5130)
                     /locus_tag="POB34_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDI5919989.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            5438..5514
                     /locus_tag="POB34_003093"
     tRNA            5438..5514
                     /locus_tag="POB34_003093"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5472..5474,aa:Val,seq:gac)
     gene            5519..5595
                     /locus_tag="POB34_003094"
     tRNA            5519..5595
                     /locus_tag="POB34_003094"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5553..5555,aa:Val,seq:gac)
     gene            5703..6008
                     /locus_tag="POB34_003095"
     CDS             5703..6008
                     /locus_tag="POB34_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HDI5919990.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            6134..7738
                     /locus_tag="POB34_003096"
     CDS             6134..7738
                     /locus_tag="POB34_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HDI5919991.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLVVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7750..8562)
                     /locus_tag="POB34_003097"
     CDS             complement(7750..8562)
                     /locus_tag="POB34_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5919992.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLAQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(8566..9351)
                     /locus_tag="POB34_003098"
     CDS             complement(8566..9351)
                     /locus_tag="POB34_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HDI5919993.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(9348..10016)
                     /locus_tag="POB34_003099"
     CDS             complement(9348..10016)
                     /locus_tag="POB34_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDI5919994.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(10080..10718)
                     /locus_tag="POB34_003100"
     CDS             complement(10080..10718)
                     /locus_tag="POB34_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HDI5919995.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(10731..12833)
                     /locus_tag="POB34_003101"
     CDS             complement(10731..12833)
                     /locus_tag="POB34_003101"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HDI5919996.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            complement(12854..13480)
                     /locus_tag="POB34_003102"
     CDS             complement(12854..13480)
                     /locus_tag="POB34_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HDI5919997.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(13936..14145)
                     /gene="fumD"
                     /locus_tag="POB34_003103"
     CDS             complement(13936..14145)
                     /gene="fumD"
                     /locus_tag="POB34_003103"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HDI5919998.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            14701..>15283
                     /locus_tag="POB34_003104"
     CDS             14701..>15283
                     /locus_tag="POB34_003104"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDI5919999.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFI"
BASE COUNT         3854 a         3886 c         3463 g         4080 t
ORIGIN      
        1 ccaccaccag cgtgatgtta tcaacagtag tgctggtcgc tctgggttac atgatgcaac
       61 gttgtgaaga agttggctgc cagaatcatg gcaatgccga agtcgctcat agcgaatcac
      121 actgatctat atcgatatac ttatacttag gctgctaaca aaattttgtt gtatgattga
      181 aattagcggc ctatactaat ttcgagttgt taaagctacg ataaatatta tgtttttacg
      241 gggacaggat cgttcccgac tcactatgga tggtcatttc ggcaaaggtt cctcctttcc
      301 ctctgttcta cgtcggatta tagactcgcg gttttttctg cgagatttct cacaaagccc
      361 aaaaagcgtc tacgctgttt taaggttctg atcaccgacc agtgatggag aaactatgag
      421 ttcatcgtgt atagaagaag tcagtgtacc ggatgacaac tggtaccgta tcgccaacga
      481 attacttagc cgtgccggta tagccattaa cggttctgcc ccggcggata ttcgtgtgaa
      541 aaaccccgat ttttttaaac gcgttctgca agaaggctct ttggggttag gcgaaagtta
      601 tatggatggc tggtgggaat gtgaccgact ggatatgttt tttagcaaag tcttacgcgc
      661 aggtctcgag aaccaactcc cccatcattt caaagacacg ctgcgtattg ccagcgctcg
      721 tctcttcaat ctgcagagta aaaaacgtgc ctggatagtc ggcaaggagc attacgattt
      781 gggtaatgac ttgttcagcc gcatgcttga tcccttcatg caatattcct gcgcttactg
      841 gaaagatgcc gataatctgg aatctgccca gcaggcgaag ctcaaaatga tttgtgaaaa
      901 attgcagtta aaaccaggga tgcgcgtact ggatattggc tgcggctggg gcggactggc
      961 gcactacatg gcatctaact atgacgtaag cgtggtcggc gtcaccattt ctgccgaaca
     1021 gcaaaaaatg gctcaggaac gctgtgaagg cctggatgtc accattttgc tgcaagatta
     1081 tcgtgacctg aacgaccagt ttgatcgtat tgtttctgtg gggatgttcg agcacgtcgg
     1141 accgaaaaat tacgatacct attttgcggt ggtggatcgt aatttgaaac cggaaggtat
     1201 attcctgctc catactatcg gttcgaaaaa aaccgatctg aatgttgatc cctggattaa
     1261 taaatatatt tttccgaacg gttgcctgcc ctctgtacgc cagattgctc aatccagcga
     1321 accccacttc gtgatggaag actggcataa cttcggtgct gattacgata ctacgttgat
     1381 ggcgtggtat gaacgattcc tcgccgcatg gccagaaatt gcggataact acagtgaacg
     1441 ctttaaacga atgtttacct attatctgaa tgcctgtgca ggtgctttcc gcgcccgtga
     1501 tattcagctc tggcaggtcg tgttctcacg cggtgttgaa aacggccttc gagtcgctcg
     1561 ctaaaggcta ttctatcgcc ccctctccgg gggcgatttc agatcaggct tctgtacctg
     1621 gttgattcat ggcattttct cgtgccgcca gcacacgttc taccgtatct accactgcct
     1681 gagtttgtgg atcaatttca atgttgacgc gtgcgccgag ttttttcttc ccaagagtcg
     1741 tgcgttccag agtttccgga attaaatgga cgcaaaaacg cgttggcgtg acttcgccga
     1801 cggtcaggct aataccgtcg atgccaataa atcctttgta caggatatat ttcatcaact
     1861 gactatcctg gactttaaac cagatctggc gattattttc cgaggttaat attttcgcca
     1921 cttcagcggt ggtcataata tgacctgaca ttaagtgtcc gccaatttca tcactgaatt
     1981 tcgccgcacg ctcaacgttt acccaatccc ccacttttaa atcgccaaga ttggtaatgc
     2041 gtaacgtttc tttcatcagg tcaaaactga catggttgcc gttaatttcc gtcacggtca
     2101 ggcagcaacc gttatgcgcc acggaagcac ctgtttccag gccgtccagc atgtggtcgg
     2161 gtaactccac cacatgcgta cgaaaatttg gtttctcgtc aatcgacacc agttttgcgg
     2221 tgccctgtac aatccccgta aacatactta caactcctga aatcagttaa gacattctgt
     2281 tcagcacaat agcaggtggc aaacgccctt accagtgaag gggtaagaat gactattttt
     2341 tcactggaga attaataaat cctcgctaca atagactgaa tttcccctgc ttcttctttt
     2401 tgctgcccat tcaggcggct ttttagtctc tcatataact acaaataaaa ggtgttcacg
     2461 tgcagaagta tatcagtgaa gcgcgtctgt tattagcatt agcaatcccg gtgattctcg
     2521 cgcaaatcgc ccaaactgcg atgggttttg tcgataccgt gatggcgggc ggctatagtg
     2581 ccaccgacat ggcggcggtc gctatcggta cttctatctg gcttccggcg atcctctttg
     2641 gtcacggact gctgctggca ttaacgccgg ttatcgcgca attaaatggt tccggtcgac
     2701 gtgagcgcat tgcgcatcag gtgcgacaag gtttctggct ggcaggtttt gtttccgttc
     2761 tcattatgct tgtgctgtgg aatgcaggtt acattatccg ctccatggaa aacatcgatc
     2821 cggctctggc ggacaaagcc gtgggttatc tgcgtgcgtt gttgtggggc gcgccgggct
     2881 atctgttctt ccaggttgcc cgtaaccaat gtgaaggtct ggcaaaaacc aagccgggta
     2941 tggtaatggg ctttatcggc ctgctggtga acattccggt gaactatatc tttatttatg
     3001 gtcatttcgg tatgcctgag ctcggtggcg ttggttgtgg cgtggctact gcggcggtgt
     3061 attgggtcat gttccttgcc atggtttctt acattaaacg cgcccgctcc atgcgcgata
     3121 ttcgtaacga aaaaggcacc gcgaaacccg atcctgcggt tatgaaacga ctgattcaac
     3181 tcggtttgcc gattgcgctg gcactattct ttgaagtgac actgtttgcc gtcgtggctc
     3241 tgttagtgtc tccgctcggt attgttgatg tcgcaggaca ccagattgcc ctgaacttta
     3301 gttcactgat gtttgtgctt ccaatgtcgc tggcggcagc ggtaactatc cgcgtaggtt
     3361 atcgtctggg tcagggctca acgctggatg cgcaaaccgc tgcgcggacc gggcttatgg
     3421 tgggtgtctg tatggcaacc ctgacggcca tattcacggt ttcactgcgg gagcaaatcg
     3481 ccctgttgta caacgacaat cccgaggttg taacgctggc tgcgcatttg atgttgctgg
     3541 cggcggtata tcagatttct gactcaatcc aggtgattgg cagtgggatt ttgcgtggtt
     3601 ataaagatac gcgttccatt ttctatatca cctttacggc ttactgggtg ctgggcttgc
     3661 caagcggcta tattctggca ctgaccgatc tggtcgttga acctatgggg ccagcaggct
     3721 tctggatagg ctttattatt ggcctgacgt cggcagccat tatgatgatg ctgcgtatgc
     3781 ggttcctgca acgtctgccg tcagttatca ttctgcaacg agcatcccgc taataaagac
     3841 aaggcgcaac cttcacgggt tgcgcctgtg tttttacgca ggctggagcg ttgcgccaat
     3901 cccgtcttcg tctggctgta atttcagagc gttgcacaga gtttgccgac tggcatctgt
     3961 tatctcaacg tgtatatccc cggtaacttc ccctttcaca ttgatgaagt caggtgagat
     4021 ttctttcgca ctggtttgca ggcaaatcgc tccctctccg cgcaggcgtt tcactttcag
     4081 ttgcacatgc gttgccgcac cagtgatcag ccacagtttt ccgccattca gattgatgtt
     4141 accaacaata aaagtgacac cgtcaccttt taccgtactg ccgtcgagaa tcaacacacc
     4201 gccctgctgt acagtaatcg ttgattgttg cagatctgcg ccatagccaa taatcattga
     4261 agcgccgtcc agtaatgcaa cactcccggt cagttcaacc ggtgcgcgat cttcctctac
     4321 gacaaaattt tcgtccgcgc tggtgggtga caacaggatc actgttccac cttgtattac
     4381 cgcattttcc agcgtaccgg catgcacttc agcccgacca cccacattca cggacaggtt
     4441 ttgcgtctta ccggaactgt agagctgaag gccatccgtc cccagacgat aaatggatcc
     4501 attctcaaca atagtgtcag agtcttcacc accgagatta ttcatggtgc caccagcaag
     4561 caacgtatca ctggctttcc cggccagcat aaaaacgccg ccctgattaa tctcacaatt
     4621 gctggcttcc ccacctggat acaccaacaa ttcaccacct tcatctacca cgaccgctga
     4681 ggttgtccca ttaaccaaca ggccgccctc ttgatcgaga atgatttttt ccgcgcgatg
     4741 tccttccagt acacgcaggt taccgccatt ttccagcaac aaaccgcagg cataaccctg
     4801 atcaacgctg aattcacctt cgggatggcg gccattaacc gtagcgagcg tagagagact
     4861 aattgcgcct ccctcggact gaacaatatc tgtggcaatg gcaccgtcgc tgaccgatag
     4921 cgtgccattg ttaccgtact gtgtgcattt ggcgatccca ccggttgcca ctttcaacca
     4981 gccagccccc gtgaccacgg tattttcggc tcttccgcca gcttcaacaa tcaactcgcc
     5041 ttcagtaagt tgcgtcgcgc caatgacctc gccggtctta acaatttgca cattcccgtc
     5101 gctcatcaag tttttcgcat cagatcccat gatttattcc tttgctgcat ctgtgtgcct
     5161 ttattgctac ctaagtgtaa aggctacgga ggatttatcc acgacagatt tgagatggtg
     5221 gcaaacaact ctgtttaaac tctgatacac gaattattgg gttgtatcag atgtaaatgc
     5281 gatcctgaat aaaaatcgcc cttgcaaatc aacaaaatat agccaatttg cttaaaagct
     5341 ctgcaaacgg tcgaaatagc gcagaaaatt acgttttgcc tcttgccacc ttcccactct
     5401 gccgctaata ttcgtccccg ttgtcaccta caacgttgcg ttcatagctc agttggttag
     5461 agcaccacct tgacatggtg ggggtcgttg gttcgagtcc aattgaacgc accatcctgc
     5521 gtccgtagct cagttggtta gagcaccacc ttgacatggt gggggtcggt ggttcgagtc
     5581 cactcggacg caccagattt tcttaatctg gtcttctcct ttttccctct gtttcttctc
     5641 tgtatccaat acgttaaaag atttacactg tcttcgtatg cgttatcaga aggagaatcg
     5701 ctatggcaac tttgttacaa cttcattttg cttttaatgg cccatttggc gacgcaatgg
     5761 ctgagcagct taaaccgctt gctgagtcga ttaatcagga acctggtttt ctgtggaagg
     5821 tatggacaga aagtgaaaag aaccacgaag ccggtgggat ctatcttttt actgatgaaa
     5881 aaagcgccct tgcctatctg gaaaaacata ctgcccgact taaaaatctc ggcgttgagg
     5941 aagttgtcgc caaagttttc gatgtcaatg agccactgag tcaaatcaat caggcaaaac
     6001 tcgcctgaca gaatttaatc aagggcggtt agcgcccttt tcatccctgt ctgaaatttc
     6061 tcaaattcta aaaatctcaa ccaaacttat ctgataacac taaattcgaa agaatgcgta
     6121 caggtaagta acaatgaaaa aaattgctat tgtgggtgcc gggcctacgg ggatctacac
     6181 cttattctcg cttctacagc aacaaactcc actttctatt tctatcttcg agcaggctga
     6241 cgaggccggt gtcgggatgc catacagtga tgaggaaaac tcaaaaatga tgctggcaaa
     6301 tattgccagt attgaaatac cgccgattaa ttgtacgtat ctcgaatggc tacaaaagca
     6361 agaagccagc catctccagc gttatggcgt taaaaaagaa accttgcacg atcgtcagtt
     6421 tttaccgcga attctgctgg gcgaatattt ccgcgatcaa tttttacgat tagtagacca
     6481 ggcacgacag caaaaatttg cagtggctgt ttatgaatca tgccaggtta ccgatctgca
     6541 aattacaaat gctggcgtca tgctcgctac aaatcaggat ttacccagcg agacgtttga
     6601 tttagtggtg attgccacgg gtcacgtctg gcctgatgaa gaagaagcaa ccagaacgta
     6661 ttttcccagt ccgtggtcag gcctgatgga agcaaaggtc gatgcgtgta acgtgggtat
     6721 tatgggaaca tccttgagcg gactggatgc ggcaatggca gtggctattc agcatggttc
     6781 gttcattgaa gatgataaac aacacgtcgt ttttaaccgc gataacgcaa gtgaaaagct
     6841 aaatattacg ttgatgtcgc gcacgggtat tttacccgaa gccgatttct attgccctat
     6901 tccctacgag cccttacaca tcgtcaccga tcaggcatta aatgctgaga ttcaaaaagg
     6961 tgaagagggc cttttggatc gggtatttag attgatagta gaggaaatca agtttgctga
     7021 tccagactgg agccaacgca tagccttaga gagcctgaat gtcgattcct ttgctcaagc
     7081 ctggtttgcc gagcgcaaac aacgcgaccc atttgactgg gcagaaaaaa atctccagga
     7141 agtcgaacgc aataaacgag aaaaacatac tgttccctgg cgttatgtca ttctgcgcct
     7201 gcatgaagcc gtacaggaaa ttgttccaca tctgaatgaa cacgaccata aacggttcag
     7261 taaaggcctt gcccgggttt ttatcgataa ttatgcggca atcccttcag agtctattcg
     7321 tcggctgctg gccttacgtg aagcggggat cattcatatt ctcgccctcg gtgaagacta
     7381 cgaaatggaa attaatgagt cgcgcaccgt cctgaaaacg gaagacaaca gctactcgtt
     7441 tgacgttttt attgatgccc gcggacagcg tccgcttaaa gtgaaagata ttcctttccc
     7501 tgggctacgc gagcaattac agaaaacagg ggatgaaatc cctgatgttg gcgaagatta
     7561 tacgttacag caacccgaag atattcgtgg acgcgtagcg ttcggcgcgt tgccctggtt
     7621 gatgcacgat cagcctttcg ttcagggact tacggcatgt gcagaaattg gtgaggcgat
     7681 ggctcgagcg gtcgtaaaac ctgcatcccg tgcacgtcgg cgtctttcgt ttgattaaag
     7741 gtaaagctat cagcactcaa caaactgatg ctgttcatta agacgatagc ggactccggc
     7801 tcgaatattg ttttcacctt caatggcgac ggcgaagcga actctttcgc cgtcatgata
     7861 tgccagtgcg gcgcttcccc ctggcccgag aataattgag tcgaccacgc ccgtgctcac
     7921 taccgttgaa ttttcaccgc tggcaatgat gtgtacattg tcgccgctgt tggcaattct
     7981 ggcgtttgcg ccaaagctgg cgatttgcgc caggtcacca ttactggcga catgacaccg
     8041 ttcacccaat gtacaaacgc gtactcgcat tccggtgttc gcaatccgac tactatctcc
     8101 agcgctgctt atccgcgaag aattaccggt tacagcaacc cgtgcgcgtt caccggagct
     8161 gccaatgtga ctgttaaagc cgacacttcc aatccgcacg ctataaccca tactggcaat
     8221 ttgcgcggca tacccagcgg ttgcgattcg ggcgttatcc tcacaacagc cgatgcgagc
     8281 atgatcgtcg ctgcgggcaa actgactgca aaatggtatc tctccggggt ctgtggccag
     8341 tttttgcata gaggaaactt cctgatgcgc gaagttttca tccgccagcc attttttcca
     8401 cgcatactca accagactct ccgcccagtc cgtatatcct tcctcaatca gtgcatgatg
     8461 aatatcggca taactaccgc cgcgtgggaa atggcgcagg aaccagcgat acatcaccgc
     8521 tccgatacgc cattctcgca ggtctgctcg tgtaatgatc attcgttagt gccggtgata
     8581 gccatcgacc atgcttttaa aggtttcgtg tggcgtacgc cccgtggtgc aaagataaag
     8641 atgaccgaag ataaaaaaga ggcttataaa tgccagagca aaatgcgcct gcaataacca
     8701 gtatcttacg ccaggaaaca catctcccac ggcttgcgga tagagacaca gcagccccgt
     8761 caatagtaac aacggcagca atccatacat aacaccaaca taggcgacct gctgtaaggg
     8821 attaaattta gactgggttg ttgccgggaa aggatgttct tccccctgca taatgccaaa
     8881 caaataaaat cgcgtttgtt ttgccgctcg ctccagccac ccctgacgac gaatgcgata
     8941 gtggtgacca ttacccccaa cggcattgat cagcacaaag ccgagccagc atgcgagtaa
     9001 caaaaatccg caaacttcat gcaccgcaac cagactttta accgcagttg cgcccaccag
     9061 cgcaaagtga tttatcagcc cacttgccag caacaataca aagagtaacg cattcgacca
     9121 gtgccataat ctgaccgctt tgcagtaaag ataaatcttc tcaccataat cggtgactga
     9181 tttcttcacg ccacgagcac gaagcaaggc atgcagcccc agcaccaacc acatcccaac
     9241 cagcaatact ccggcaatga tcaaccacac cggccaaaat tcgggagtga actgtggcac
     9301 atagttcgct aactggctct gaaactgttc agcatgttgc gacgggttca tacattttcc
     9361 tttttaatca aatgttgacc gaaccggcga tacagatgcg gtttcccggc accaggtagc
     9421 tgatattgat agtatttatt ttgctgcaac cacgcctgaa tttctgggct atcttcgcga
     9481 ccaaagatca atgcgtgttc cggacaggcg ctgacgcaaa tgggcggaaa gccttttgcc
     9541 agtcgggact cagcgcagaa atcacattta tctgccactt tagtgacagg gttaaggtag
     9601 cgaacctggt acggacaagc gccaatgcaa taactgcagc cgatgcactg cgatttttct
     9661 acccgcacga tcccctgctc atcacgccac gacgcccctg tcgggcatac gtcgatgcac
     9721 ggagcatctt cgcagtgctg acatgactgg cgaaaaaagt gatactgcgt ttcgttgtcg
     9781 ttatcggtga ctggaatgtg tgctatcgat aaacggcttc cctgagcggg gacgtggtta
     9841 gttttacggc atgcgcgggc gcaaatatta cagccattgc accgtgactc gtcatgaatc
     9901 atggcatacc gaacttcctt ttcttgcctc gtgttcgcta acagagaaga cgcagaacca
     9961 gtaaaaaaga ttactgttcc catccccaga acaaatttgc gtcgagtgaa ggacatctga
    10021 ttatccccta atgcagtggc tgcaactgtc caccctatcc atgacatctc cctgaaattt
    10081 caggggttct gaaactgctc tacctcgaac cattcgcata agccataagc cttgccatag
    10141 gtcataaatc gctcgctaaa caagtacaac accgccgtcg atgactccac ctgccgtata
    10201 tcggcatatt caggtgcaac atccatcgcg gcgatcgcgg cttcaatctg cgcttcctgg
    10261 aagtaatatg gcgcttgcat cagcatggcg actttgtaag gacgaggata cgtctggcac
    10321 tcaaaacgaa cggcgtgtgc aatggcgcga caaatgtcct gctccacaac ttgcaaagac
    10381 atcgccgcgt agttttcact catggcctgc gttgagtaat agtaatcatc ctggcttccc
    10441 gtcatacaag cgatatccgc gtagtctccg ttctgcttaa tgctttccag caaaactgac
    10501 agctcctctt ctgcaaccga atagggtggc gagagaaaca cggcgcgcgc caccagctgc
    10561 ccagccgcag aatgctggcg aatgaactct gccaggattt cagccggttc tggttcggtt
    10621 accgtatcaa cggggtgacg catcccctgt aaccattgcc gcttcgcctc gtcaacttcg
    10681 ctgactttcg ctaacggcaa ttcatcccga tggttcatct cacccattcc ttacacaggc
    10741 agtagattgt gtgctgccag atcggcggca atatcttcca gccccagacg ctgcaatgtt
    10801 tcgcgggtcg gacaaccaag ctgtgggtcc cagcccattt ctttgtagaa catcgtgagc
    10861 gaagcatgca tatcgtcacg atccattttg tcagtacctt cagtaaagac cgggatctgc
    10921 ggatccttgt cgaataccca ggaacagata agatcgtgtt cattacgcat atctttggtt
    10981 tgcatcagtt ttaccgtgta ggcacgatgc aacgtaaaga tacgctctgc agctaaatct
    11041 aatttttcct gagtcatctc ttcgccggtg atcgctttga agaatttggc ttcaagcgcc
    11101 agatcgcccc ggtaattacg gcttttcagc ggcgaaacgg tcattggcca gacccagttg
    11161 cacagggtga cggcgttatg caaacagacc cgcaacagcg accactttgc atatttaatt
    11221 tttgcgtcat tgattggcgt gtagtttttg gtttcatcgt aggcatcttc agaaccaaaa
    11281 agctctttcg ccacttcacg ctgcagtttc aatggcaaac ccgaaccaat aaaattgata
    11341 tgggtatgcg tcatgcagtc acggttgaac atacagttaa caatggaacc gacctgcgct
    11401 gacgcttcat tggcatggtg aaccggatag ccaaacggcg accagagttt attttttgcg
    11461 tagccccagt actcttcacc caaattccag cgttctgcga tggcatatga accatcagcc
    11521 aggtgactca gctcacccac acgatgcgcc agacggtagt aaaaatcttt aatgaaatta
    11581 acgtcacccg cttccagttg atcccagtga atttccgcat actcttcagc tggcagaaca
    11641 cgcttgaaca cccctttgct gtaacaataa gtaaagtcgc gatgcagctg cccgtagtta
    11701 caccataggc cgtagtcatc gaacaaattc agaccaacca ggttaccaat cacacgacca
    11761 tcgtctttat cttcaaaatc tttcggcccg ttcgggaaga tggtggtatg gacaaagttg
    11821 gctacacaag tgttaccacc tgtgctgggc acgccaaact ctttcacccg aggaatattc
    11881 atttgggtca tacaacggat cgggcaagag tggcagccac tcatttttac tgtgtatttc
    11941 tctgccgccg gacctaagtc aaaaacggat ttataggtac gaaagccgac cgtgttctga
    12001 ttgcctggcg gaatttcccc cgtttcaatc ggaccacctt cagccgcgcc ccaaaacagc
    12061 cctttacgtg ctgtccaacg tgacttggga tctgaatact ctgcccacga ttgcggcgta
    12121 cttggcacga catggttgtt attcgcacca ataagttcag tcatcatgta atcgttgaga
    12181 cgcttcatct cctgacgatc ggcaatgttg acccctttcg tcccttcaac cgcaatcgct
    12241 ttcaggtttt tcgaacccat aattgcgcca gttcccgcac cgccgctgtg gttacggcta
    12301 ttcaacatgc cagagagagg aacaaggttt tccccagcct gaccaatagc cgccacacag
    12361 gtttccggac tggtcaatcg acaaatttct tccgtcgttg cgcgcgtccc ttttccccat
    12421 aagaaatcgg ctttttccag gctaactttg tcatctttaa tcttcagcca taccggtgat
    12481 ttcgccttcc cttcgataat aatgacgtcg tatccagcga atttcatttg cgctgcaaaa
    12541 aagccaccca tatgggcatc gacaactaaa tttcctttgg taaaagtaga aagtgaggtg
    12601 atatttacgc gagaactaca gggggcacca gatccagtta atgggccggt agcaaagact
    12661 aatttattcg cttcatcgaa aggtttcgtg cctggcggta cttcgtcata cataattttg
    12721 tagccgaagc ccatgccacc gacaaaactt ttaaacttac tggaatcttc gagggtaata
    12781 tttcctgtcg tgagattgac tcttaatata ttacctgtcc aaccgttagc catgattttt
    12841 tcctttgcaa gattcacaca gtaatatctt tccactcgat aatttttaac gcccctgttg
    12901 ggcaggcgtt tgcgcattca ccgcataaca cacatttcga ggatttttta ctttcggtat
    12961 ttacggtggc catcatccac gggcatgccg tagtacaggc gctacagcca atacagcgtt
    13021 tatgatcgac ggtaatacag ccttctttct gctgccaggt aatcgcacca atcgggcaga
    13081 cgttcatgca ttgcggctct ttgcattgac ggcaggtgtc cgcggtatag ttgagatcgc
    13141 catacaggcc gccgccagag ccaaccccgt tgtcgccaaa gaaataattg cgatggattt
    13201 tgatacggga gaagaatgtc cctactgagc catcgttgaa gttggtacaa gagatttcac
    13261 agcggtggca acccgtgcat cgcgctcgct gggtcaccag cacccctttg ggtgtattta
    13321 tcaaccctac tgtgccgcta tcaatatctt cctgtttgca acccaaaagt gacagtaacg
    13381 caggggcaat tgttagacca gcaaggcctt ttcctgatat acgcaagaat tctaatcgcg
    13441 ttaagccaat atctaatagt ggacgatcaa ccgggttcat ttatttcgca tcctcttcgc
    13501 aaacacagtg ctgacaattc agcagcctga tattaagcat tcaactaata cgctgctgac
    13561 tctgtcccca acaccagata ttttccctaa cccgatagtg tatgagaaaa gatttaacgc
    13621 cgataaacta atcctttatg gttaggagaa aaaataatgt gattattctg agcccttaac
    13681 attgatcgtt atcaattaaa attacaaagc aagaaattat aagtgaactg atatttatta
    13741 tcatttgaaa taaatttaac ttaagcgaca attatttgtg acgttcattg cagaaaataa
    13801 aaataatatt atcgttataa attaaaatac ataccgcaat tcgtataatt tcagcagttg
    13861 ttatttagaa tgattaactg gaccgcaact gaagcaagaa tatcaggatg aagcttaccg
    13921 cctcatcctg aatggtcatt ttaccagtgc gttaatgacg gtttccatcg cctgcttttc
    13981 cagctcactg cgctgaatgc gtttattggc taatgctgta cgtaatacgc ctgcgataac
    14041 aatatgcttt ggttcctgcc ccagatcgcg catttccagc acaactttac cgacaactct
    14101 gcacatttct cggtacagtt catcctcttt tgtccgattt cccattcttt atacctattt
    14161 atcatgccaa ctatcagcat atatcaatct aaccaattaa acaaaaatca aacaaaatca
    14221 gacaaataac gcgataaatt attttaactg tagcaattga gcgatgatat atttatacac
    14281 cggatgaact ttcacttatc ctcacactga caacttcggc accagacgtt gcgcaaacag
    14341 tgaagttttt gcgtaacctt ttccctggaa cgttaaatct ttgataacaa tttattgtct
    14401 aacaagttgt atttttttga aacgctgttt ttgttttcct tttggattaa tttcagcgta
    14461 taatgcgcgc caattgactc ttgaatggtt tcagcacttt ggactgtaga actcaacgac
    14521 tcaaaaacag gcactcacgt tgggctgaga cacaagcaca cattcctctg cacgcttttt
    14581 cgatgtcacc tatccttaga gcgaggcacc accactttcg taataccgga ttcgctttcc
    14641 ggcagtgcgc ccagaaagca agtttctccc atccttctca acttaaagac taagactgtc
    14701 atgaaaaaga ccaaaattgt ttgcaccatc ggaccgaaaa ccgaatctga agagatgtta
    14761 gctaaaatgc tggacgctgg catgaacgtt atgcgtctga acttctctca tggtgactat
    14821 gcagaacacg gtcagcgcat tcagaatctg cgcaacgtga tgagcaaaac tggtaaaacc
    14881 gccgctatcc tgcttgatac caaaggtccg gaaatccgca ccatgaaact ggaaggcggt
    14941 aacgacgttt ctctgaaagc tggtcagacc tttactttca ccactgataa atctgttatc
    15001 ggcaacagcg aaatggttgc ggtaacgtat gaaggtttca ctactgacct gtctgttggc
    15061 aacaccgtac tggttgacga tggtctgatc ggtatggaag ttaccgccat tgaaggtaac
    15121 aaagttatct gtaaagtgct gaacaacggt gacctgggcg aaaacaaagg tgtgaacctg
    15181 cctggcgttt ccattgctct gccagcactg gctgaaaaag acaaacagga cctgatcttt
    15241 ggttgcgaac aaggcgtaga ctttgttgct gcttccttta ttc
//
LOCUS       DAMZET010000099        15263 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.099, whole genome shotgun sequence.
ACCESSION   DAMZET010000099 DAMZET010000000
VERSION     DAMZET010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.099"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..651
                     /gene="caiD"
                     /locus_tag="POB34_003105"
     CDS             <1..651
                     /gene="caiD"
                     /locus_tag="POB34_003105"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HDI5920000.1"
                     /translation="QLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTE
                     IFNLDKPVIAAVNGYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLP
                     KILPPAIVNEMVMTGRRMGTEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIA
                     ALKEIYRTTSEMPVEEAYRYIRSGVLKHYPSVLHSEDAVEGPLAFAEKRDPVWKGR"
     gene            657..1247
                     /gene="caiE"
                     /locus_tag="POB34_003106"
     CDS             657..1247
                     /gene="caiE"
                     /locus_tag="POB34_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HDI5920001.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(1333..1728)
                     /gene="caiF"
                     /locus_tag="POB34_003107"
     CDS             complement(1333..1728)
                     /gene="caiF"
                     /locus_tag="POB34_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HDI5920002.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(1989..5210)
                     /gene="carB"
                     /locus_tag="POB34_003108"
     CDS             complement(1989..5210)
                     /gene="carB"
                     /locus_tag="POB34_003108"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDI5920003.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(5228..6376)
                     /gene="carA"
                     /locus_tag="POB34_003109"
     CDS             complement(5228..6376)
                     /gene="carA"
                     /locus_tag="POB34_003109"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDI5920004.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(6832..7653)
                     /gene="dapB"
                     /locus_tag="POB34_003110"
     CDS             complement(6832..7653)
                     /gene="dapB"
                     /locus_tag="POB34_003110"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDI5920005.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKEGGLFDMRDVLDLNSL"
     gene            complement(7820..8734)
                     /gene="rihC"
                     /locus_tag="POB34_003111"
     CDS             complement(7820..8734)
                     /gene="rihC"
                     /locus_tag="POB34_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HDI5920006.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            complement(8800..9750)
                     /gene="ispH"
                     /locus_tag="POB34_003112"
                     /gene_synonym="lytB"
     CDS             complement(8800..9750)
                     /gene="ispH"
                     /locus_tag="POB34_003112"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDI5920007.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKSAFLIDDAKDIQEEWVKEVKCIGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(9752..10201)
                     /gene="fkpB"
                     /locus_tag="POB34_003113"
     CDS             complement(9752..10201)
                     /gene="fkpB"
                     /locus_tag="POB34_003113"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDI5920008.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(10326..10820)
                     /gene="lspA"
                     /locus_tag="POB34_003114"
     CDS             complement(10326..10820)
                     /gene="lspA"
                     /locus_tag="POB34_003114"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDI5920009.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(10820..13636)
                     /gene="ileS"
                     /locus_tag="POB34_003115"
     CDS             complement(10820..13636)
                     /gene="ileS"
                     /locus_tag="POB34_003115"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDI5920010.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(13679..14620)
                     /gene="ribF"
                     /locus_tag="POB34_003116"
     CDS             complement(13679..14620)
                     /gene="ribF"
                     /locus_tag="POB34_003116"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDI5920011.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(14628..14846)
                     /locus_tag="POB34_003117"
     CDS             complement(14628..14846)
                     /locus_tag="POB34_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HDI5920012.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            14949..15212
                     /gene="rpsT"
                     /locus_tag="POB34_003118"
     CDS             14949..15212
                     /gene="rpsT"
                     /locus_tag="POB34_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDI5920013.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         3563 a         4250 c         3899 g         3551 t
ORIGIN      
        1 caattacgtg tcgccattat taccggtgcc ggagagaagt tcttttccgc gggctgggat
       61 ttaaaagcgg cagcagaagg cgaagcaccg gatgctgact ttggcccggg tggatttgcg
      121 ggattaaccg aaattttcaa tctcgacaaa ccggttatcg cagctgtgaa cggctatgcc
      181 tttggcggcg gtttcgaact ggcgctggcg gcagatttta ttgtttgtgc cgataacgcc
      241 agcttcgccc tgccggaagc caagctgggc atcgttcctg acagcggcgg cgtgctgcgt
      301 ctaccgaaga tcctgccgcc tgccatcgtc aatgaaatgg tgatgaccgg cagacgaatg
      361 ggcacagaag aggcgctgcg ttgggggata gtcaaccgcg tggttagcca ggcggaactg
      421 atggataacg cccgcgaact ggcgcagcag ctggttaaca gcgccccgct ggcgattgcg
      481 gcgctgaaag agatctaccg caccaccagc gaaatgccgg tagaagaagc gtatcgctat
      541 attcgcagcg gcgtgttgaa acactatccg tcggttctgc attcggaaga tgccgttgaa
      601 gggccgctgg cgtttgccga gaagcgcgat ccggtgtgga aaggacgtta acaaccgtga
      661 gctattacgc ctttgagggg ttaattccgg tggttcaccc gacggcgttt gtccatccca
      721 gtgccgtctt gattggcgat gtgattgtgg gagccggtgt ctacatcggc ccactcgcct
      781 cactgcgtgg tgactacggg cggttgatcg tgcaagcggg agccaatatt caggatggct
      841 gcattatgca tggctactgc gacactgaca ccatcgttgg ggaaaacggc catatcgggc
      901 acggagcgat cctgcatggt tgtgtgattg gtcgcgatgc attggtcggg atgaacagcg
      961 tgattatgga tggcgcggtc attggcgaag agagcattgt tgccgccatg agctttgtca
     1021 aagcaggctt tcacggcgag aaacgccagt tgttgatggg tacgccagcc cgcgctgtac
     1081 gcagtgttag tgacgacgag ttacactgga aacggttgaa taccaaagag tatcaggatc
     1141 ttgtcgggcg ctgtcatgca tcgttacatg aaacacagcc actgaggcaa atggaggaaa
     1201 atcgcccccg tttgcagggg acgacggatg tgacgcctaa acggtgatct tgccggatgc
     1261 gacctggttg ctggataaga tgctacaaca tcgcatctgg caagcaggac gcccggtatc
     1321 aacgagataa aattaacgac gcatactctt tgacaacatc atctgccact tctgctcgcg
     1381 gttaagttcc gtgagcggaa cggcaggaat acgcggcgtc tttcttacgc cgactaattt
     1441 atcccggctg ttattgcgca ggcgcgcgta aatttgctca tcgatatcga ccacttttac
     1501 cagtcgctgg cactggcatc cccgtccttc cagcttatta gggatcatct taacttcgca
     1561 gcttatttct gcgacttccg acagaatata agtcagggta ttaaccgcct tgctgtgttc
     1621 aatatcgaaa tgaatagaga tctctcttgc tatcacccac cgattttcag ccatcatcca
     1681 ttcggcgatt aacaagtaga gtggtttttc aacatatcct tcacacatac gaactccagt
     1741 ttcacaaatt aaaagtctgg ctatgactcg ttttgaagca attaaacaag gccatattaa
     1801 aattgtggat atatcgatag tatgcaactt caccgtcgat aagtgtgagc taaatcctgc
     1861 ttatgtcatc aaaaatatcg attccccaaa agatttactt aatgggcaaa aaaaacggtc
     1921 ctcatcagag aaccgtatta cttattcgat caaattagcg gatgaaaaat atccgccatg
     1981 acacgctatt atttgatctg cgcgtgcatt tcctgcaccg aaattacttt ttcggtcgcg
     2041 tcggcattca gcgccatcgc ggtagcgaaa ccaccgttca gggtggtgtc ataatgcact
     2101 ttatattgca gcgcactgcg acggatcacg cgggagtctt caatcgcgcg acggcctgaa
     2161 gtggtgttga tgatgtaggt atattcgcca ttcttgatgc ggtcctgaat gtgcggacgg
     2221 ccttcatgca ccttgttcac cagacgcgga ttaatgcccg cttcgcccag cacaatcgcc
     2281 gtgccgtggg tcgcatccag ctcgaagccc tgtttcagca gttttgccgc caggtccacc
     2341 acgcgttctt tatcgccttc gcgcacggaa agcaacgcac gaccgtgttt cttcatggtg
     2401 gagttgctgc ccagctgcgc tttggcaaac gcttcagcga aggtgcggcc cacgcccatc
     2461 acttccccgg tggagcgcat ttctggccct aacagcgggt caacacccgg gaatttattg
     2521 aacggcagca ccacttcttt caccgagtag tacggcggga taacttcttt ggttacgccc
     2581 tgctcagcca gcgatttgcc agccatcaca cgcgccgcca cttttgccag cggtacgccg
     2641 gtggctttgg agacgaacgg aacggtacgc gccgcacgcg ggttaacttc aatcaggtag
     2701 acttcgttgt ttttcaccgc aaactgcacg ttcatcagac cgcgcacctg caattcgaag
     2761 gccagtttct gcacctgctg gcgcatcaca tcctgaattt cctgacttaa ggtgtaggct
     2821 ggcagagaac atgcggagtc accggagtgc acgcccgcct gctcgatgtg ctccatgatg
     2881 ccgccaatca gcaccatttc gccgtcgcag atggcatcca cgtcaacttc taccgcgtca
     2941 tcaaggaagt gatccagcaa cactggcgca tcgttagaca cgctgaccgc cgtctggaag
     3001 tagcgacgca ggtcagcttc gtcatagacg atttccatcg cccgaccgcc gagaacgtaa
     3061 gacggacgta ccaccagcgg gtagccaatc tctttcgcct tctctaccgc catttcaata
     3121 gcggtaacgg tggcgttcgc cggttgtttc agtttcagac gctcaaccgc atgctggaag
     3181 cgttcacggt cttctgcacg gtcgatagca tccgggctgg tgccgataac cggtacgcca
     3241 gcagcttcca gcgcgcgcgc cagtttcagc ggggtctgac cgccgtactg aacgataacg
     3301 cctttcggct tctcaatacg cacgatttct agcacatctt ccagagttac cggctcgaag
     3361 tagaggcgat cggaagtgtc gtagtcggtg gagacggttt ccgggttaca gttaaccata
     3421 atggtttcgt aaccgtcttc gcgcagcgcc agcgaggcgt gtacacagca gtagtcgaat
     3481 tcgataccct gaccgatacg gttcgggccg ccgccgagaa ccatgatttt ttcacggtcg
     3541 gtagacggat tcgcttcgca ctcttcttca taagtggagt acatgtaagc ggtgtcggtg
     3601 gcgaactctg ccgcacaggt atccacgcgc ttataaaccg ggtgcaggtc atactggtcg
     3661 cgcagcttgc ggatttccgc ttcgcgcacg cccgccagtt ttgccagacg cgcatcggca
     3721 aagcctttgc gtttcagctg gcgcaggaat tcagcgttca ggccagtgat gcccacttcc
     3781 gctactttct cttccagacg caccagttct tcaatctgta ccaggaacca gcggtcaata
     3841 ttggtcaggt tgaacacgcc gtccacagac aggcccgcac ggaaggcatc ggcgatgtac
     3901 cagatacgct ctgcgccagc atctttcagt tcgcgacgga ttttggttaa cgcttccggg
     3961 tcatcgaggc tcactttcgg gtcgaatcca gtcgcaccga cttccaggcc gcgcagcgct
     4021 ttttgcaggg attcctgctg cgtgcgacca atcgccatca cttcgccaac cgatttcatc
     4081 tgagtggtca gacggtcgtt agcaccagcg aatttttcga agttgaagcg aggaatttta
     4141 gtaaccacat agtcgatgga cggctcgaag gaggccggag tacgtccgcc agtgatgtcg
     4201 ttcatcagtt cgtcgagggt gtaacccacc gccagtttcg ccgccacttt agcaatcggg
     4261 aaaccggtcg ctttcgacgc cagcgccgaa gaacgggaca cgcgtgggtt catttcgata
     4321 acaatcaggc gaccgttttt cgggttcacc gcaaactgga cattggaacc accggtttca
     4381 acgccgattt cacgcagcac cgccatcgag gcgttacgca tgatttgata ttctttgtcg
     4441 gtcagcgttt gggctggcgc gacagtgatg gagtcaccgg tatggatgcc catcgcatca
     4501 aagttttcga tagagcagac gatgatgcag ttgtcgtttt tatcacgcac cacttccatc
     4561 tcgtactctt tccagccgat cagcgactca tcaatcagca gctctttggt tggggagaga
     4621 tccagaccgc gggcgcaaat ttcttcaaac tcttcgcggt tataagcgat accgccgccg
     4681 ctaccgccca tggtaaagga tgggcgaata atgcacggga agcccacgtc agcggcaacc
     4741 gccagcgctt cttccatcgt gtgtgcgata ccggaacgcg cggtttccag accaattttc
     4801 ttcatcgcta cgtcgaaacg acggcggtct tctgctttat caatcgcatc ggcggtcgca
     4861 ccaatcatag tcacgccgaa ctcttccaac acgccctgac gctccagctc cagcgcgcag
     4921 ttcagcgccg tctgaccgcc catcgttggc agcaccgcgt ccgggcgctc tttttcaata
     4981 atcttgcgta ctacttccca gtgaatcggc tcgatgtagg tcgcatcggc catttccggg
     5041 tcggtcatga tggtcgccgg gttggagttc accagaatga cgcggtaacc ctcttcgcgc
     5101 agggctttac atgcttgcgc gccagagtag tcaaactcac acgcctgacc gataacaatc
     5161 gggcccgcac ccagaatcag gatacttttt atatctgtac gttttggcat ggctctttta
     5221 ctcctgatta cttagcggtt ttacggtact gctcaattaa ctcgataaag tggtcgaaca
     5281 acggcgctgc gtcgtgtggc ccagggctgg cttccgggtg accctggaag ctgaacgccg
     5341 gtttatcagt gcgatgaatg ccctgtaacg taccgtcgaa cagggattta tgcgtgacac
     5401 gcaggtttgc aggtaatgtt gcttcatcca ccgcaaaacc gtggttctgg gcggtgatca
     5461 tcaccacgtt tttctccaca tctttgaccg gatggttgcc gccgtggtga ccaaatttca
     5521 ttttgacagt cttcgcaccg ctcgccagcg ccagcagctg atgaccgagg cagatgccga
     5581 ataccggaat atcggtttcg aggaatttct ggatggcggt aatggcgtaa tcgcacgggg
     5641 ccgggtcgcc aggaccgttg gagaggaaga tgccgtctgg attcattttc agcacatctt
     5701 ccgcagaagt ttgcgccgga acgatggtca ggcgacagcc tctatccacc agcatccgca
     5761 ggatgttgcg cttggcacca aaatcataag ccacgacgtg gaacggcagc tcgtcttctt
     5821 ttttcgcttc tggcaggccg ccggtcaacg tccagctccc ttgtgtccag ctataggctt
     5881 ctgcggtggt cacttctttt gccagatcca tgccattcag acctgggaac gcgcgggctt
     5941 tttctaacgc caacgccgca tccgggttat cgcctgcgat aatgcagcca ttctgtgcgc
     6001 ctttctcgcg cagtaaacgc gtcagcttac gggtatcgat atcggcaatc gccacgatgt
     6061 tatggcgctt caggtaagaa gaaaggtctt cggtattacg gaagttgctg gcaatcagcg
     6121 gcagatcgcg aatcaccaga ccttgtgcat gtacctgaga agattcttca tcggcgtcat
     6181 tagtgccgac attgccaata tggggataag taagagtaac gatttgacga gaataggaag
     6241 gatcagtgag gatttcttga taaccggtca ttgaagtatt gaaaacgact tccccaaccg
     6301 ccgaacctgt tgcccctatg gcccgaccgt gaaactgggt tccgtcttcc agaaccaata
     6361 gcgctgactt aatcaaaaca ccctccagag aatattcact cactttattt gcatattaat
     6421 tcacaatgat gacgtaaatc aatgcaaatc tgcttaccga cgaatttctg gcaaacggcg
     6481 gcattctgga gatatgggcg ctaaaagtca acttaatggc gggatttttt atattatttt
     6541 gtggctcatg catgttttac aggcattaag cgacaaaaag atcatataac ctgttctgga
     6601 aaacgcttgc gcagcaagaa gattgcatga aataaaagcg agcagggaaa agtggaccaa
     6661 atggtcaaaa tttacattaa aacaacaaaa accgaagaca aaacatgtaa tttttggcac
     6721 ttatagacaa tttaaaaaca taacactaaa aataaaaggg ccaaaaatta aagcccttta
     6781 taatcaataa attaaatgtg ttattcttgc accataacaa atattttgtg gttacaaact
     6841 attgagatca agcacatctc gcatatcaaa aagaccacct tccttaccac tcaaccacaa
     6901 agccgatctt accgcgccgt tagcaaatgt catgcggctg gacgccttat gggtgatctc
     6961 cagacgctcg ccaatatcgg caaacatcgc ggtatgttcg ccaacgatgt cacctgcacg
     7021 cacggtggca aaaccaatgg tgccaggcac acgttcaccg gtgtggcctt cacgactgta
     7081 gaccgcgcaa tctttcagat ctttatcaag ggcgtgggcg atcgcctctc ccattgccag
     7141 tgcggtgcct gacggcgcat caactttatg tctatgatgt gcttcaataa tttcgatatc
     7201 ggtgtagtca cccatcactt tggctgcttt ctccagcagc ttaagcatga cgttaacgcc
     7261 aacgctaaaa ttggcggcaa agacaatcgc aatatcggcg gcggcgtcac gaatcgcctg
     7321 tttaccggct tcgtcaaacc ccgtagtgcc gatcaccatc cctttgccat gctggcgaca
     7381 aaaagcgaga tggttcagcg taccttccgg acgggtaaaa tcgataaaca catcaaaatc
     7441 atcttttacc gcatcgaggc tgctttgcac ggtaacgcct gttttccctg caccagccag
     7501 ctcaccggcg tcgctgccca gtaaagaaga tccttcgcgc tccagcgccg cgcccagctg
     7561 cacgccttct aatgccagcg ccgcctgaat caactggcgg cccatacgcc ccccggctcc
     7621 cgcgatggca acgcggatgt ttgcatcatg catagctatt ctcttttgtt aatttgcata
     7681 gaccgttttc agagtaacca gcgcatcgct acgccgccag ccaaaaacgc tgataattag
     7741 aaattaatga taaacaggga gggatatcag tgaaaggcat gagtcgatga atgactgcat
     7801 gccgataaca tgtgacaggt tacgacgcca gagccagcac ctcagccacc cactgctgga
     7861 agcctttcac atccagatcc aatgccacct ttacattggc tggtttgccc aggcaaccgt
     7921 cgatatcaac caccgtcgtg cctgaggtaa attcgccttg agtttctacc gcgacaaaac
     7981 acggcttgag ggtgaataag tccgggcgca ccagccaggc gatggcgcag agatcgtgca
     8041 ttcgcagacc gctttgcata ctgccgctac ggtagtggct aaacagtgca tgaagcattt
     8101 ttccggtgcg attcaactcc ggcaaagtag cgagatagtc aggggttaat attgcctgat
     8161 tggtgacatc cagaccgcac atgacgattt cgattccgct acggaagaca caggcagccg
     8221 cttctggatc ggcagcgata ttaaactcgg cgtttggcgt acagttgccg cgtccggcag
     8281 aaccacccat gatcaccaga cggcgaatat acggcttgca ttccgggcat tgtgaaagta
     8341 acagcgcaat attggttaac gggccaatgg ccaccagggt aacaggctct ggtgcacgca
     8401 tcagggcatc ccgaatcgcc agaaacgccg gtatcccgag cggctttcgg ttgtgctcaa
     8461 caaagtcgta gccagccatt cccgattcgc cgtgcacaga tgccgcatca cgcggtgcgc
     8521 gtaccagcgg cacagcggcc ccttgggcga gcggaatctc cgcattccag aaatgcagca
     8581 gttgcagggc attgcgggta gttttctcaa ccgagacatt acccgcgacg gtggtcatca
     8641 gttgcaggtc gagttcgggt gcaaaaatcg cggcggcaat ggcgacggca tcgtcaatgc
     8701 cggggtcagt atcgaggaag ataggtaaac gcatgttttc tccataaaaa atgccggtaa
     8761 caagaccggc attttcgcat aacttaggct gctaatgact taatcgactt cacgaatatc
     8821 gacacgcagc tctttcggca cttcgaaaac aatgttttct tcacgtcctt ccagcggaat
     8881 ggcttcacca ccgcccagct gttgcaaacg tgccaccaca ttctgcacca gaatatccgg
     8941 agccgatgcg cccgcagtca cgccgatgca tttaacctct ttcacccact cttcctggat
     9001 atctttcgca tcatcaatca aaaacgcgct tttgcccata cgctgggcca gctccgccag
     9061 acggttggag ttagaggagt ttttcgaacc gaccaccagc acaacttccg cctgttcggc
     9121 cagggcacgt accgcttcct gtcggttagt cgtggcgtag cagatgtcat ctttgcgcgg
     9181 accgacaatt ttcgggaagc gtttacgcag cgcgtcgatc acatcagacg tgtcatccac
     9241 cgacagcgtg gtctgggtca taaaggagag tttctcttcg tttttgaccg tcagtttcca
     9301 cacatcgtct ggtgattcaa ccagatacat tcccccttcc gggttgctgt actgacccat
     9361 cgtgccttcc acttccgggt gcccggcgtg accgatgaga atagattctt cgccacggcg
     9421 actagcgcgg gcgacttcca tatgcacttt ggtcaccagc ggacaggtgg catcaaacac
     9481 cgtcaaatcg cggctttttg cttcgttacg taccgcctga gaaacaccgt gtgcggagaa
     9541 aatcaggatc gcgccgtccg gtacttcgct aatctgctca ataaagatag ccccacgctc
     9601 acgcaagcta tcgaccacat agcggttatg taccacttcg tgacggacat atatcggtgc
     9661 gccgtaaatg gccagcgcgt tttcaacaat gctgatagcg cggtctaccc cggcacaaaa
     9721 accacgcggg ttggccaaca ggatctgcat gttacgcctc cagtgccgga tcgatttcca
     9781 gcacttcaat atcaaaatga acggtctgcc cggccagcgg atggttgaaa tcaacggtaa
     9841 tggagtcgcc gttaatttcg cggatcacgc caggcatctc actgccatcc attgcggtaa
     9901 aaagcatgat tgcgccaatt tctggctcgc ctgcatccat aaattcacgg cgggagaagt
     9961 actgaatcag gtccggtgac ggcacgccaa acgccgcatc tggctccaac gagaaggtgg
    10021 ttttatcgcc cactttcagc cccaacaggt gttgctccag cccttcagaa agagaagcat
    10081 cacccaggcg gaacagcgcc ggtttaccgt tgttgcgggt agactcggcg gtggtgccat
    10141 cgtcgagttt tagcgtgaag tgcaccagga cggcgctatt gctctgtaca gattcagaca
    10201 tgcaggtttg ctcttatatt taaggatttt tgccggatgc ggcgtaaacg ccttatccgg
    10261 cctacgattt cgcagcaatc tgtaggccgg ataagatgcg tcagcatcgc atccggcagg
    10321 gtttattatt gttttttcgc tttagaaggt aaaaaacctt ccagcacaat cagtgccgca
    10381 ccgacacaaa tggcagtatc ggcaaggttg aaggtggcga agtgccagtc gccgacgtag
    10441 aagtcgatca tatcgacaac gaagccgtgc cacaggcggt cgaacaggtt gcccagcgcg
    10501 ccgccaataa tcagcgcgta agcgatattg tttagcttct gcgtggcctt cgagcgatac
    10561 atcatcactg ccaggatcac gctaatacca atcgcaatac cggcaaagaa ccaacgctgc
    10621 caaccgccgc tatcggcaag gaaactaaac gccgcgccat agttacgcgc ataatgcaga
    10681 ttaagcgacg ggaacagcgg gaccgtatcc cccagagcaa agttctggag gatcaggtat
    10741 ttgctgccca gatcgataat cagcacgact accaccagcc acagccagcg tagccctgtt
    10801 gaacagatcg attgactcat caggcaaact tacgtttttc accgtcaccg gcgacgttgc
    10861 tgacacagcg gccgcagatt tctgcgtgtt ccgccacctt gccgacatcc tgggtgtagt
    10921 gccagcagcg tgggcacttc tcaccttcgg ctttactcaa cgcgactttc agccctttga
    10981 gtacttcgct ctgctgagca tcagcaggtg cgtcgttata gtctgcaacg gtagcgccgg
    11041 aggtcaacag gacaaatcgt aattcatcgc ccagcgcggt cagtttcgcc gacagttccg
    11101 gttctgcata caaggttact gccgcttcca gcgagccacc cactttcttg tcggcacgcg
    11161 cttgctcaat gactttgttc acttcgccac gcactttcaa cagctcgtcc cagaacgcat
    11221 cgttcatcgc ttcactgtct gccaggccaa acaggccttc gtaccactca ccggtgaaga
    11281 cgtatttttc acgttcgccc ggcaggtagc cccacacttc atcagcggtg aaggagagga
    11341 ttggtgccat ccagcgcacc agcgcttctg cgatgtgata cagcgcagtc tggcagctac
    11401 gacgcgccac actgtccgct ttggcggtgt actgacggtc tttgatgatg tcgaggtaga
    11461 aggaacccat ctcaacggag cagaagcgca tcagacgctg taccacttcg tggaaatcgt
    11521 atgcttcgta cgccttgagg atgtcttcct gtgccgcttt cgcacaacct acggcccagc
    11581 gatccagtac caccatctct tccggtttca ccatatcttt tgctggatca aaaccgttca
    11641 ggtttgccag caggaagcgc gcggtgttac ggatacgacg atagctatcg gcagcacgtt
    11701 tcaggatctc gtcagaaacg gccatttcac cggtgtagtc ggttgatgcc acccacagac
    11761 gcagaatatc cgcgcccagt ttgttcatca catcctgcgg cgaaacggta ttgccgatgg
    11821 atttagacat cttgcggccc tgaccatcca cggtaaagcc gtgggtcagt acctgacgat
    11881 acggcgcttt gcctttcatc gcggtggaga tcattaggga agacatgaac caaccacggt
    11941 gctggtcaga accttccaga tacatgtccg ctgcgtgacc ggcaaattcc ggacgcacgt
    12001 caacaacaga agagtgggta gatccggagt caaaccatac atccaatgtg tccggcactt
    12061 tcacgtactg atcagcttca tcgccgagga tctctttcgc atcaagatcc caccacgcct
    12121 ggatgccgtc aacttcaacg cgttttgcca cttcttccat tagttcgaga gtacgcggat
    12181 gcagctcttc cgtgtctttg tgcacgaaca gtgacatcgg tacgccccag gtgcgctgac
    12241 gggagataca ccagtcagga cggttagcaa ccatcgactc gatacgcgcc tggccccagt
    12301 ccgggatcca ctgcacgcct ttgatctctt tcagtgactg cgcacgcaga cctttctgat
    12361 ccatgctgac gaaccactgc ggcgtcgcgc ggaagatgat cggcgttttg tgacgccagc
    12421 agcacggata gctgtgctgc attttctcaa cgtgcagcag cgcgcctttt tcctgcagca
    12481 gcgcaacgac gatgtcgttc gctttgaaga cgttcacgcc atccagcgtc ggataagtgc
    12541 ccggcagata agtgccgtcc gggccaaccg ggttagcggt ttccaggccg tatttctgac
    12601 cgatcacata gtcgtccggg ccgtggccag gcgcggtgtg aacggcaccg gtaccggcat
    12661 ccagggtaac gtgatcgccg aggattgccg gaacgtcgaa gcccataaac ggatgggtaa
    12721 agcgcagcaa ctcaagctcc gcacctttta ccgtgccgag aatggtgtaa tcggtcacgc
    12781 cgatacgctg cattacgctt tcaaccagat ctttcgccag aatcacggcc tgaccgtcga
    12841 tctgcaccag cgcatagtcg aaatcaggtg caatagagat tgcgcggttc gcaggcagag
    12901 tccacggcgt agtggtccag atcaccagcg agattgggcc gttaacgttg cttacggcaa
    12961 attttgcttt cagtgcatcc tgatcgaccg cctgaaaagc aacgtcgatg gacggagaag
    13021 ttttgtcgta atactcaact tccgcttccg ccagcgcaga acggcagtca acgcaccagt
    13081 gtaccggctt cgcgcctttg tgcaggtgac cgttgccgat gattttgccc agcgcgcgga
    13141 tgatgttggc ttcagttttg aagtccatgg tcaggtacgg gtgcgaccag tcgcccagca
    13201 cgcccagacg gataaagtct ttgcgttgac cgtcaacctg ggtcgccgcg tattcgcggc
    13261 acttggcgcg gaactcggcg gcggtgaatt tctcacccgg cttaccgtat tcttgctcta
    13321 ctttcagctc gatcggcaga ccgtggcagt cccagccagg cacatacggc gagtcataac
    13381 cggaaagccc tttggacttc acgataatgt ctttcagaat cttgttaacc gagtgaccaa
    13441 tatgaatgct gccattcgca taaggagggc catcatgcag aatgaaggtt tttttgcctt
    13501 ttttagccgc acggatgatg ccgtacagat catcatcagt ccaacgcgcc agcattcccg
    13561 gttcgcgctt ggcgagatcg ccacgcatcg ggaaccctgt ttccggcaaa ttcagggttg
    13621 atttatagtc actcatcaga ttctcggttc cgtgtttcgg tttgattaca taacaggctt
    13681 aagccggttt tgttagccca aaaaattcgc gggcggttaa ttcatcacgc gcaatctgcg
    13741 ctttcagttc gtccagcgac gcaaatcgct gctcattgcg tattttttta cgcagcacta
    13801 cttgtatatg gcgaccgtaa aggtccattg caacatctaa caaatgcact tccagctgct
    13861 ggcgaatacc ggcaaccgtt gggcgtgttc cgatgtttgc cacgccgggt aacggctttt
    13921 caccgaggcc cagcacttct accgcataaa cccctttcac cggggaaacc tgacggcgta
    13981 gcggtacatt cgccgtcggg aaacctatag tgcgccctaa ttcatcaccg tggactacac
    14041 gcccggagat ggcaaacggg tgccccagta aactttctgc cagagccaga ttgtcatccg
    14101 ccagcgcctg acgcacagcg gtgctgctaa tacgcacgcc accttcgcaa aaagtttgcg
    14161 tactagtgat atcgaagccg tattccatgc cagctttctg taataacaag aaatcgcctt
    14221 cacgaccagc gccaaagcgg aaatcatccc ctacggcaag aaattttacg cgcaaatgct
    14281 tcaccagaag atcgctgatg aaattttgcg cggttaacgc cgcgaaacgc ctgtcgaaac
    14341 gcacgcacag cacgtaatca acgccacact ctgcaaggta acgcagtttt tcccgcagcc
    14401 gggtcagtct tgccggggct ttatcggtag caaacagttc cagtggttga ggttcaaaaa
    14461 gcatcaccat caccggtaag ttgcgcttgc gcccttcttc ctgcaagccc tgtaacagcg
    14521 cgcgatgacc gcgatgcacg ccgtcgaaat taccaatagt cagcacacac ccttcttgcg
    14581 gggcctggct gagattatgt atgccgcgta tcagcttcat gtctggctca aaacagtgaa
    14641 aatcgtccga gtataccttg tacagcggtc aaggttaacc ggcgattgag taccgaaatc
    14701 agaaagaagt ggcttttcat tgccatggcg caaatcacgg gaagaaagtg accgcctgct
    14761 gcaattttta tcgcggaaaa gctgtattca caccccgcaa gctggtagaa tcctgcgcca
    14821 tcactacgta acgagtgccg gcacattaac ggcgcttatt tgcacaaatc cattgacaaa
    14881 agaaggctaa aagggcatat tcctcggcct ttgaattgtc catatagaac acatttggga
    14941 gttggacctt ggctaatatc aaatcagcta agaagcgcgc cattcagtct gaaaaggctc
    15001 gtaagcacaa cgcaagccgt cgctctatga tgcgtacttt catcaagaaa gtatacgcag
    15061 ctatcgaagc tggcgacaaa gctgctgcac agaaagcatt taacgaaatg caaccgatcg
    15121 tggaccgtca ggctgctaaa ggtctgatcc acaaaaacaa agctgcacgt cataaggcta
    15181 acctgactgc acagatcaac aaactggctt aatcgccaat ttgctgaagc tttgtgaaaa
    15241 agcccgcgca agcgggtttt ttt
//
LOCUS       DAMZET010000100        15236 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.100, whole genome shotgun sequence.
ACCESSION   DAMZET010000100 DAMZET010000000
VERSION     DAMZET010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.100"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..2816)
                     /gene="yfaL"
                     /locus_tag="POB34_003119"
     CDS             complement(<1..2816)
                     /gene="yfaL"
                     /locus_tag="POB34_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HDI5920014.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAVTDSCQGYDVKASCQ
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGGGGAIDVTDNNSDSK
                     HPSGYTIVNNTAFTNNTAEGYGGAIYTNSVTAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSSAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQN
                     QAELNVGSTQQTFVHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANN
                     NSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITSDVDTRSDAAGHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADILPYASSLWVGDGATFVTGADQDIQSIDAISSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSNVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPTPDPDPTPDP"
     gene            3512..5797
                     /gene="nrdA"
                     /locus_tag="POB34_003120"
     CDS             3512..5797
                     /gene="nrdA"
                     /locus_tag="POB34_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDI5920015.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            6032..7162
                     /gene="nrdB"
                     /locus_tag="POB34_003121"
     CDS             6032..7162
                     /gene="nrdB"
                     /locus_tag="POB34_003121"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDI5920016.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            7162..7416
                     /gene="yfaE"
                     /locus_tag="POB34_003122"
     CDS             7162..7416
                     /gene="yfaE"
                     /locus_tag="POB34_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HDI5920017.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(7470..8120)
                     /gene="inaA"
                     /locus_tag="POB34_003123"
     CDS             complement(7470..8120)
                     /gene="inaA"
                     /locus_tag="POB34_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HDI5920018.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            8299..8541
                     /locus_tag="POB34_003124"
                     /pseudo
     CDS             8299..8541
                     /locus_tag="POB34_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            9007..10077
                     /locus_tag="POB34_003125"
     CDS             9007..10077
                     /locus_tag="POB34_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920019.1"
                     /translation="MPNTSIHLSRCNILQNNKLQPEEVYKESQQTAKLEIFCDEFLKI
                     SQSRYGLSTSADSIANLLTFFTKASDAIDRIKTQKIDVHSYGFVPIRHFVEHVITYKN
                     EFAADYEPYTLTFTRGENNEGVLSIESKEGSISQRTINLNEYETAINIINEHVTKENI
                     HNTVQSLTEKDISKINSSDKHHKISSEESIKSQLYSDQKKYADLLLHSEKNTEWYKYA
                     SSEERYDKFKNSSKEIKNTYKQIVLAQKKLNQMKYINKLGGELIDIADKKLAPLINDS
                     FSYTRDFFAYSKQENNIFTFDNSKFVDPKEKEGLMIQHSNGQLVITGKYCPEGVQTAF
                     TQEQYDKLIRYINIFFTFPKCE"
     gene            complement(10296..11372)
                     /gene="glpQ"
                     /locus_tag="POB34_003126"
     CDS             complement(10296..11372)
                     /gene="glpQ"
                     /locus_tag="POB34_003126"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDI5920020.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(11377..12735)
                     /gene="glpT"
                     /locus_tag="POB34_003127"
     CDS             complement(11377..12735)
                     /gene="glpT"
                     /locus_tag="POB34_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDI5920021.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            13008..14636
                     /gene="glpA"
                     /locus_tag="POB34_003128"
     CDS             13008..14636
                     /gene="glpA"
                     /locus_tag="POB34_003128"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HDI5920022.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            14626..>15236
                     /gene="glpB"
                     /locus_tag="POB34_003129"
     CDS             14626..>15236
                     /gene="glpB"
                     /locus_tag="POB34_003129"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HDI5920023.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPL"
BASE COUNT         4017 a         3940 c         3501 g         3778 t
ORIGIN      
        1 tcaggatccg gcgtgggatc aggatcggga gtcgggtctg gatcaggtgg cgatggcggc
       61 gttacttctt gcgatcgcag ataccagtcg ttgttatctt ccaccagcgt gtagtcatac
      121 gctcccatat tgacgtagcc gctgcctgcc agactgaact gcgcattgtt ttgaaactgc
      181 gtgggatcag ctgcgaaatc aaccacttta atgcctgtcg atgtcggctc accaatccct
      241 gtaatggagt taaccaccac agttgtgttg ccagcagtat taccgttcat caccaattga
      301 tcgcttaccg agtcatcgcc gtttaattcg ctatcgagca gtagcgtacc gccgccggta
      361 taatcaccgt ttaccgtcag cgtatcgcca gcgacgccat tttgcaggct aaccgcaccg
      421 gacgtattgg tcagattgcc attaaccgtc acgttggaaa gataagtcag gctgtcagtt
      481 tcaaggttgg tattaagctc acctgtcaac gtcacaccat cggtggcatt caccagcgtc
      541 ccatcgccgt taaatagtga ggcattaagg gcgacggaag tatcctgccc ggtcaggcgc
      601 aaaaccgtac catcgctgat gtcgatagtg ccgctggaaa tagcatcaat tgactgaata
      661 tcctgatccg cgccagtaac gaacgttgcc ccatcaccaa cccacagtga cgaagcataa
      721 ggaaggatat cagcaacatc acctttcagg gtgccttctt cgacacgtac cgccgactgc
      781 gttgtaccgc tggcggtcag ctccagtgta ccagcccccg ttttagtcaa tgtgctaccc
      841 tcatcctgat gctgcccgct gctgtcagcc atcagtgcgc cccactgcgt gtctaccccc
      901 gcatcaactg ccacttcacc gtcggcgcgc atttcaatat cacgtccgtg gcctgccgca
      961 tctgaacgcg tgtcaacgtc ggaggtaatc tccatcacgc tttcttgttg cttatcggta
     1021 aagataacct ggcggttata gtgggtatct ccaagctgcg agttgtcgct caccatcagc
     1081 gtaccggagg cgatttgtgt tgtgcccaaa taactattat tgtttgccag acttacctgc
     1141 ccgtcgctcg catcgaccac cacgtagacg ccatctttcc cgtcgccgcc gatatgcaaa
     1201 ttgtcgccac ctgcaagatc taccacatcc tgactgccga taaccgttcc gctgctgcca
     1261 ctgacttcaa ggccatcgtt gtatgatgtg ccgctctgcc aggtggagaa atcagagaga
     1321 tcgagcacac cgccgtttaa cacgatcgac tgcggatcgt cctggagagc ggtaagatct
     1381 gccgcgtcgc cttccagcga aagcaccgca ccatcatcaa cgactatatc gccggttagc
     1441 gccatcgact gcgcccctgc cagcacgtag ctgccgtttt gcgcaatggt gagctgacca
     1501 gcaccttcga tgatgccagc aaaactgccc tgattaacag taacgttgcc accagcatcg
     1561 atatttaaag tgccattctg aaagcccgtc aatgcgtgca caaaagtttg ttgggtcgag
     1621 ccaacgttta gctcagcctg attctgatac tgatcaatac tccctatcgt cagaccgtag
     1681 cagtcttgcg gatcgtcctg gcaatgcgta tcgccgacat tcatcaggga gttgctgcgg
     1741 cccagggtaa cttcaccgtt ttcaatctgc atctcaccag taaagtcatt gttatctgca
     1801 ttaagtacca gatcgccgga acctgttttg gtgattaacc cggtaccagc aatagagtca
     1861 acagctccgt cattctctgt attgccaata accagcgttt ttccgtcggc aatatcaaag
     1921 gtaacttcac ttaagccgag atacataaag ccacccgccg cagaggaagg accatctcca
     1981 tagcctgctg cgctattgtt ctcatcgact aacacgcctc cgttctggct gtagctgtca
     2041 tcaacagaaa tatcaataag atagggagcc gtcacgctat tggtatatat cgccccgcca
     2101 taaccttcgg cagtgttatt tgtaaaggca gtgttattta ctatcgtata acctgaagga
     2161 tgcttgctgt cgctattatt atcggtaaca tcgattgccc cgccaccgcc atcactgtaa
     2221 cttgttgatg tatatgcctg gttgttatca aaaataacat cacttaaata aacgtcatta
     2281 ttaatggtat aaattgcgcc acctttgcca tcattagcga tgttattgcg aaacatggcg
     2341 ttagtgacac gtaaatcgac ggcaccagta tcgttagtac cagaagaata gattgcgcca
     2401 ccatagccgc ctgcgacgtt accggaaaaa ataacatcag taagattcag cgttgagttt
     2461 tctttagcaa atatggcccc gccgttatta tattctcctg taacggtgtt attggcaaac
     2521 agagtcattc cagtttcatt ttctggtaat agtgaaaatt ccgctccttg ttgcaaaaat
     2581 acggccccac cgctggcgtt attggtcata tccgaaaaaa ccagccattg cccatcagcg
     2641 atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcctgaca actcgctttg
     2701 acatcatatc cctggcatga atcagtgacc gccgcgacac cgttagcaga gaaaagagat
     2761 gcaatcattg acgggagtaa agataaatac tccttgcgta gaaagataat ccgcatatta
     2821 atctaaccat cattttctat aagacggcgt atttaatcgc attatacgat atggaatatc
     2881 tttcttttgt aaattgttca acccggtgca atggctattt tgttattatt tgatgaataa
     2941 tatcagtgcg tcataattca agttaataac cttcagggat atcagttata tttaaactaa
     3001 attaaagtca tgaataattt tcttataata taaggtaaat taacaaaatg gcttagcatt
     3061 taacaataac cgaatagaaa acaaccattt cgccatcaac aatctcttac attcgcttat
     3121 atattgacca caactgatac atcagattat gtgatgactc gtgcttagat caatttttgc
     3181 aatcattagc aaaaagatta ataaaccatc tatatcaatt tatctaacct attatgccgt
     3241 tcaagaaatc gccgaacagt tatttttaac aaatttttct cttcccattg actttcccgg
     3301 acaccttgtc tgacctaagg tgcgcgaaag ccactttttc cttcctgagt tatccacaaa
     3361 gttatgcact tgcaagaggg tcattttcac actatcttgc agtgaatccc aaacataccc
     3421 cctatatata gtgttctaag cagcttcccg tactacaggt agtctgcatg aaactattgc
     3481 ggaaagaatt ccaaaaacag gtacgacata catgaatcag aatctgctgg tgacaaagcg
     3541 cgacggtagc acagagcgca tcaatctcga caaaatccat cgcgttctgg attgggcggc
     3601 agaaggactg cataacgttt cgatttccca ggtcgaactg cgctcccaca ttcagtttta
     3661 tgacggtatc aagacctctg acatccacga aaccattatc aaggctgccg cagacctgat
     3721 ctcccgtgat gcgccggatt atcagtatct cgccgcgcgc ctggcaatct tccacctgcg
     3781 taaaaaagcc tacggccagt ttgagccgcc tgcgctgtac gaccatgtgg tgaaaatggt
     3841 cgagatgggc aaatacgata atcatctgct ggaagactac acggaagaag agttcaagca
     3901 gatggacacg tttatcgatc acgaccgtga tatgaccttc tcttatgctg ccgttaagca
     3961 gctggaaggc aaatatctgg tacaaaaccg cgtaaccggc gaaatctatg agagcgccca
     4021 gttcctttat attcttgttg ccgcgtgcct gttctcgaac tacccgcgtg aaacgcgcct
     4081 gcaatacgtg aagcgttttt acgacgcggt ttccacattt aaaatttcgc tgccgacgcc
     4141 aatcatgtcc ggcgtgcgta ccccgactcg tcagttcagc tcctgcgtac tgatcgagtg
     4201 cggtgacagc ctggattcca tcaacgccac ctccagcgcg attgtgaaat atgtttccca
     4261 gcgtgccggg atcggcatca acgccggacg tattcgtgcg ctgggcagcc cgattcgcgg
     4321 tggcgaagcg ttccataccg gctgtatccc gttctacaaa catttccaga cagcggtgaa
     4381 atcctgctct cagggcggtg tgcgcggcgg tgcggcaacg ctgttctacc cgatgtggca
     4441 tctggaagtg gaaagcctgc tggtgttgaa aaacaaccgt ggggttgaag gcaaccgcgt
     4501 gcgtcatatg gactacgggg tacaaatcaa caaactgatg tatacccgtc tgctgaaagg
     4561 tgaagatatc accctgttca gcccgtccga cgtaccgggg ctgtacgacg cgttcttcgc
     4621 cgatcaggaa gagtttgaac gtctgtatac caaatatgag aaagacgaca gcatccgcaa
     4681 gcagcgtgtg aaagccgttg agctgttctc gctgatgatg caggaacgtg cgtctaccgg
     4741 tcgtatctat attcagaacg ttgaccactg taatacccat agcccgtttg atccggccat
     4801 cgcgccagtt cgccagtcta acctgtgcct ggaaattgcc ctgccgacca aaccgctgaa
     4861 cgacgtcaac gacgagaacg gtgaaatcgc gctgtgtacg ctgtctgctt tcaacctggg
     4921 cgcaattaat agcctggatg aactggaaga gctggcaatt ctggcggttc gtgcacttga
     4981 cgcgctgctg gattatcagg attacccgat cccggccgcc aaacgtggag cgatgggtcg
     5041 tcgtacgctg ggtattggtg tgatcaactt cgcttactac ctggcgaagc acggtaaacg
     5101 ctactccgac ggcagcgcca acaacctgac gcataaaacc ttcgaagcca ttcagtatta
     5161 cctgctgaaa gcctctaatg agctggcgaa agagcaaggc gcgtgcccgt ggtttaacga
     5221 aaccacttac gcgaaaggga tcctgccgat cgatacctat aagaaagatc tggataccat
     5281 cgctaatgag ccgctgcatt acgactggga agctctgcgt gagtcaatca aaacgcacgg
     5341 cctgcgtaac tccacgcttt ctgctctgat gccgtccgag acttcttcgc agatctctaa
     5401 cgccactaac ggtattgaac cgccgcgcgg ctacgtcagc atcaaagcgt cgaaagacgg
     5461 tattctgcgc caggtggtgc cggactacga gcatctgcac gacgcctatg agctgctgtg
     5521 ggaaatgccg ggtaacgatg gctacctgca actggtgggt atcatgcaga aatttatcga
     5581 tcagtcgatc tctgctaaca ccaactacga tccgtcacgc ttcccgtcag gaaaagtgcc
     5641 gatgcagcag ttgctgaaag acctgctcac cgcctacaaa ttcggggtca aaacgctgta
     5701 ttatcagaac acccgtgacg gcgctgaaga cgcgcaggac gatctggtgc cgtcaatcca
     5761 ggacgatggc tgcgaaagcg gcgcatgtaa gatctgatat tgagatgccg gatgcggcgt
     5821 aaacgcctta tccggcctac ggctcggatt tgtaggcctg ataagacgcg ccagcgtcgc
     5881 atcaggctcc gggtgccgga tgcagcgtga acgccttatc cggcctacgg ctcggatttg
     5941 taggcctgat aagacgcgtc agcgtcgcat caggcacagg atgcggcgta aaatgcctta
     6001 tccggcatta aactcccaac aggacacact catggcatat accacctttt cacagacgaa
     6061 aaatgatcag ctcaaagaac cgatgttctt tggtcagccg gtcaacgtgg ctcgctacga
     6121 tcagcaaaaa tatgacatct tcgaaaagct gatcgaaaag cagctctctt tcttctggcg
     6181 tccggaagaa gttgacgttt ctcgcgaccg tatcgattac caggcactgc ctgaacacga
     6241 aaaacacatc tttatcagca acctgaaata tcagacactg ctggattcca ttcagggccg
     6301 tagcccgaac gtggcactgc tgccgctgat ctcgatcccg gaactggaga cctgggtcga
     6361 aacctgggcg ttttcagaaa cgattcattc ccgttcctat actcatatca ttcgtaatat
     6421 cgttaacgat ccgtctgttg tgtttgacga tatcgtcacc aacgagcaga tccagaaacg
     6481 tgcggaaggg atctccagct attacgatga gctgatcgaa atgaccagct actggcatct
     6541 gctgggcgaa ggtacccaca ccgttaacgg taaaactgtg accgttagcc tgcgcgagct
     6601 gaagaaaaaa ctgtatctct gcctgatgag cgttaacgcg ctggaagcga ttcgtttcta
     6661 cgtcagcttt gcttgttcct tcgcatttgc agaacgcgaa ttgatggaag gcaacgccaa
     6721 aattattcgc ctgattgccc gcgacgaagc cctgcacctg accggcaccc agcatatgct
     6781 gaatctgctg cgcagcggcg cggacgatcc tgagatggcg gaaattgccg aagagtgtaa
     6841 gcaggagtgc tatgacctgt ttgttcaggc agctcaacag gagaaagact gggcggatta
     6901 tctgttccgc gacggttcga tgattggtct gaataaagac attctctgcc agtacgttga
     6961 atacatcacc aatatccgta tgcaggcagt cggtttggat ctgccgttcc agacgcgctc
     7021 caacccgatc ccatggatca acacctggct ggtgtctgat aacgtgcagg ttgctccgca
     7081 ggaagtggaa gtcagttctt atctggtcgg gcagattgac gcggaagtgg acaccgacga
     7141 tttgagtaac ttccaactct gatggcccgc gttaccctgc gcatcactgg cacacaactg
     7201 ctgtgccagg atgaacaccc ttcccttctg gcggcgctgg aatcccacaa tgtggcggtt
     7261 gagtatcagt gtcgcgaagg ttactgcggc tcctgtcgca cacgcctggt tgcaggtcaa
     7321 gttgactgga ttgccgaacc gttagccttt attcagccgg gggaaatttt gccctgttgt
     7381 tgccgggcaa aaggcgatat tgaaatcgag atgtgaattg gttgtagtgc cagatacaac
     7441 gcttatgcgt cttatctggc ctacaacgat tacatggcgt agtaatacgc tttcacctgc
     7501 tcccagtccg ctttggggat tggctccaga tatttttcca gctggcggaa gtcatgatta
     7561 atcgctttat cgcgacgcaa gcggcgacga cttttctcca ggtcaagaaa accagcttct
     7621 gccttacctt ctgttttcac atagatatgg cgaacataac aacagccatg ctgacgatta
     7681 atgctatgca ttttcttaaa cgccagcgcc actgctttca acatggcttg ccgtacttcg
     7741 tcagaataag gcgatactgc atgctgggca taccagtcag caatgctgat gaaccccgcc
     7801 atatcttcag tcaccagcaa cgctcgccat tcaccctcaa ttttcaccgc ttcaccaaaa
     7861 acgatcttcg gcacgatgac accggcccgt tccagttctt taattaccgc aacctcacgg
     7921 acaatcgttg gtcggccgaa cggataacgt acggaatgaa acagatgatg cgtcatgcgc
     7981 tttacataca gctttttgcc gttgcgctcg acgcattgca ccccgctcat accattacgg
     8041 cgatagttag gctcttcaac ccagtcgccc tctgttgccc accagtgatt aaattcgtcg
     8101 tactttgctg aaactgccat accctatcgc ctgtcatttt tattaacgac aatgactata
     8161 ggtggttacc tgaggaaaat cttaatgaaa cgtgtcgtat taatgaattt cttttgccac
     8221 tactagcgtg acaccgcttt tacccttcat ttgcagcgca tagattccac cagccactac
     8281 cactaatagt gcgcccagca gataaaatgt gaatttaatc aggcggatac agcgatgaat
     8341 tttattcgac agggattagg catcgcctta caaccagagt taacgctgaa aagcattgca
     8401 ggtgaattgt gttccgttcc tctcgaatcg accttctatc gacagatttc gttgttggct
     8461 aaagaaaagc cggtagaagg cagtccactg tttttactac aaacgtgtac ggaacaactc
     8521 gtggtgagtg gaaaaatttg atgtgagaca tgagattgat gacaaatgta agattgcctg
     8581 attcgccaca cttagttggc ctgtagtctt taccaggcgt aataattcgt tttcacctga
     8641 ttttggtcca ctttgtgaat tagcgatata tatttatcca gtttagggaa gtgacaatga
     8701 ctccaggtag aaaccggtat gaagcgcccg actcactgtc aaaaccgatt gttgagagta
     8761 atgacactcc ggcgaacagt acacagttta ctctggagcc attctcaatc ttcaagtggg
     8821 atacctaata acttgtcagg gaagttatca atatctgttt gttacttatt accgtgttta
     8881 ttaacaataa tgatactttc taacatgcgt aaatgtcttc gtaataaaat gagctaaatt
     8941 tgatagtgaa tatattctgc aaagattaaa ttgtatcttg ttaactcaca atacaaggaa
     9001 tcatttatgc caaatacatc tattcactta tctcgctgta atattttaca aaataataaa
     9061 ttgcaaccag aagaagttta caaagaatca caacaaaccg cgaaacttga gattttttgt
     9121 gatgagtttc taaaaatatc tcaaagtaga tacggattat caacctcagc agacagcata
     9181 gctaatttat tgactttttt cacgaaagcc tcagatgcaa ttgatagaat caagacccaa
     9241 aaaattgacg ttcacagtta tggtttcgta ccaataagac attttgtcga acatgtcata
     9301 acgtataaaa atgaatttgc agcggattat gaaccctata cccttacctt tacccgaggt
     9361 gaaaacaacg aaggtgtttt gagtatcgaa agcaaagaag ggtctatttc acaaagaaca
     9421 ataaacttaa atgaatatga aactgcaatt aacatcatca atgaacatgt tacaaaagaa
     9481 aatatccaca acacggtcca gtcactgaca gaaaaagaca tttcaaaaat aaacagctct
     9541 gataaacatc ataagatcag cagtgaggaa agtattaaaa gtcaacttta cagcgatcaa
     9601 aaaaaatatg cagacctgct actacattct gagaaaaata cagaatggta caaatacgca
     9661 tcctccgagg aacgctatga taaattcaag aattcttcta aggaaattaa aaacacctac
     9721 aagcaaattg tactagcgca aaaaaaattg aaccaaatga aatacatcaa taaattaggt
     9781 ggtgagctta tcgatatcgc cgacaaaaag ttagcaccat taataaatga tagtttcagc
     9841 tatacacgag atttctttgc ttactcaaaa caggaaaata acatcttcac atttgataat
     9901 tctaagtttg tagatccaaa ggaaaaagaa ggtttaatga tacaacacag caatgggcaa
     9961 ctggttatta ctggtaaata ttgccctgaa ggtgtgcaaa cagcattcac ccaagaacaa
    10021 tatgataaat taataagata cattaatatt ttctttactt ttccgaaatg cgaataatac
    10081 tttacagtat aaactgcaat aaaattaatt attgaaccca attcatactg tttaaagaat
    10141 aacaataccc agtgttagct tccctaacga atgagtttta cgcaaatatg ttggcatttt
    10201 tttaacagga tgcaccggtt aatgaataga acaacggtag tagcattatt tcgctttagc
    10261 agaggatggc gcatgaacgc catcctgcaa tagcattact ctttattaag gaattttact
    10321 gccttatcag ggaaatcagt aaacagccca tttacacccg ctttgttata cagagcatca
    10381 tataactgat tcacatcagt agtgtattca ggcagtttat ccgaccgcac ggtataagga
    10441 tgcactacca gtttattctg ctgagcatct tgcaccatgc cagtgagttt gatattaccc
    10501 ggctgcgatg tttcctcaat caacatatgg taatccggac caataccatc tgcatattcc
    10561 gccacctgtt tcatggcacc cggcttaaac atccagtcgt agttgtaatt aacccagctt
    10621 ccatccggct gtttctgctg cgtttcattc cagtcggtat aggctatcag ctgtaccaga
    10681 ttgagctcca tgcccatttt gggttccagc tcattcttaa tacgcttcag ctcatcagca
    10741 tcaaaacatt gcaaataaac tttatcgtct ttaccggtgt aaccatattt cttcagcact
    10801 tccagcgttt ttgccgcaat atccttccct tcctgatgat ggaaccacgg cgctttgatt
    10861 tctggataaa taccgatatt tttcccggta gagtgattta acccctgaac aaattcaatc
    10921 tcttcttcaa aggtgtgcac ccggaagtcg gacttaccca ttgggaaacg ccccggataa
    10981 gtctgcactt ttttaccgtt ttcaatatcg aaaccttcgg taaatttcag cgacttaatt
    11041 tcatccagcg tgaaatctat cgcgtagtaa cgaccgtctt tgcgcgcccg atccgggaaa
    11101 cgatcggcaa catcagtaac acgatcgaga taatggtcat gcagaacaac cagatggtcg
    11161 tctttggtca tcaccaaatc ctgttccaga taatccgctc cctgcgcata cgccatcgct
    11221 tttgctggca gcgtatgctc cggcaaatat ccactggcac cgcgatgggc gatgactatt
    11281 ttttcgttgc tgtccgccgc cattgcactg cttcccatga ctatagtgct catcatgatc
    11341 gccatgctaa ggtttttcag cgtcaatttc atgccattag cctccgttgc gtttttgcag
    11401 taattgttca tggcgacgtt tttcgccaat catcacaaca atcaacaaga taaccgccag
    11461 aatgctgccg ccaatcatta ccataaagcc gccatcccag ccgaagaagt ccacggtgta
    11521 gccaacaatc gcgctcgccg ccaccgaacc gcccaggtaa ccaaacagcc cggtaaagcc
    11581 cgctgccgta cctgccgctt ttttcggtgc cagttccagc gcatgcagac cgatcagcat
    11641 cacaggaccg tagatcagga agccgataac aatcatacaa atcatatcga cggttgggtt
    11701 acctgccggg ttcatccagt aaacgatagt cgcgatggtc accagtgtca taaagaaaac
    11761 gccggttgcc ccacggttgc cacggaagac tttatccgac atccagccgc acagcagagt
    11821 gcccggaata cctgcatatt cataaaggaa gtaggcccag gaggatttat ccagcgcgaa
    11881 atgcttaacc tctttcagat aagtcggtga ccagtcgagg atgccgtaac gcagcagata
    11941 aacgaacacg ttggcgatgg cgatatacca cagcagtttg ttcggcagta cgtactgcat
    12001 gaagatttgc ttcgccgtca gctcctgttc cgctttttcg ttatagtcgt ccggataatc
    12061 atttttgtac tcttcgatcg gcggcaagcc acaggattgc ggggtatcgc gcatcatcgc
    12121 aaaggcgaat aatgccacca gaatggcgca gaaagcgggc atatagagcg ccgcgtgcca
    12181 gtcattgaac caggccatcc ccagcaggaa cagcagcggc ggaataccac caccaacgtt
    12241 gtgcgcacag ttccacactg acacaatgcc gccacgttct ttctgcgacc accagtgcac
    12301 catagtacga ccacacggcg gccaccccat cccctggaac caaccgcaga ggaacaaaag
    12361 tacaaacatc accgcaatgc tcgacgtcgc ccatggcaca aagcccataa acaacatcac
    12421 tgccgccgcc agaatcaaac ctgcggggag gaaaacgcgc ggattcgagc gatctgatac
    12481 cgaacccatt atgaatttcg aaaatccata agcgatcgag atccccgaaa gcgcgaaacc
    12541 taaatcaccg cgagagaatc cctgctcgac cagataaggc atagcaagcg caaagttctt
    12601 acgaaccaaa tagtaagccg catagccaaa gaatatcccc aggaaaattt gccagcgcaa
    12661 tcgacgataa gtcggatcga tctccgcagc aggtaagcgc gctttgtgtg gcgctggttt
    12721 aaaaatactc aacattgaaa gcctccgtgg cccgtggtct tatttatgat taacagactg
    12781 attcagtggg agaacctgcc gtttctttag ttgccgcgat gttaagaaaa cattcagaaa
    12841 ttaaatgtga attgccgcac acattattaa ataagattta caaaatgttc agaatgacgc
    12901 atgaaatcac gtttcacttt cgaattatga gcgaatatgc gcgaaatcaa acaattcatg
    12961 tttttactat ggctaaatgg taaaaaacga acttcagagg gataacaatg aaaactcgcg
    13021 actcgcaatc aagtgacgtg attatcattg gtggcggcgc aacgggagcc gggattgccc
    13081 gcgactgtgc cctgcgcggg ctgcgcgtga ttttggttga gcgccacgac atcgcaaccg
    13141 gtgccaccgg gcgtaaccac ggcctgctgc acagcggtgc gcgctatgcg gtaaccgatg
    13201 cggaatcggc ccgcgaatgc attagtgaaa accagatcct gaaacgcatt gcacgtcact
    13261 gcgttgaacc aaccaacggc ctgtttatca ccctgccgga agatgacctc tcattccagg
    13321 ccacttttat tcgcgcctgc gaagaagcag ggatcagcgc agaagctata gacccgcagc
    13381 aagcgcgcat tatcgaacct gccgttaacc cggcactgat tggcgcggtg aaagttccgg
    13441 atggcaccgt tgatccattt cgtctgaccg cagcaaacat gctggatgcc aaagaacacg
    13501 gtgccgttat ccttaccgct catgaagtca cggggctgat tcgtgaaggc gcgacggtgt
    13561 gcggtgttcg tgtacgtaac catctcaccg gcgaaactca ggcccttcat gcacctgtcg
    13621 tggttaatgc cgctgggatc tgggggcaac acattgccga atatgccgat ctgcgcattc
    13681 gcatgttccc ggcgaaagga tcgctgctga tcatggatca ccgcattaac cagcatgtga
    13741 tcaaccgctg ccgtaaacct tccgacgccg atattctggt gcctggcgat accatttcgc
    13801 tgattggcac cacctcttta cgtattgatt acaacgagat tgacgataat cgagtgacgg
    13861 cagaagaggt tgatattctg ctgcgtgaag gggaaaaact ggcccccgtg atggcgaaaa
    13921 cgcgcatttt gcgggcctat tctggcgtgc gcccgctggt tgccagcgat gacgacccga
    13981 gcggacgtaa cgtcagccgt ggcatcgtgc tgctcgacca tgctgaacgc gatggtctgg
    14041 acggatttat caccatcacc ggtggcaaac tgatgaccta tcggctgatg gctgaatggg
    14101 ctaccgacgc ggtatgccgc aaactgggca acacgcgccc ctgtacgact gccgatctgg
    14161 cactgcctgg ttcacaagaa cccgctgaag ttaccttgcg taaagtcatc tccctgcctg
    14221 ccccgctgcg cggttctgcg gtttatcgtc atggcgatcg cacgcctgcc tggctgagcg
    14281 aaggccgtct gcatcgtagc ctggtatgtg agtgcgaagc ggtaactgcg ggtgaagttc
    14341 agtacgcggt agaaaattta aacgttaata gcctgctgga tttacgccgt cgtacccgtg
    14401 tggggatggg tacctgccag ggcgaactct gcgcctgccg cgctgccgga ctgctgcaac
    14461 gttttaacgt cacgacgtcc gcgcaatcta tcgagcaact ttccaccttc cttaacgaac
    14521 gctggaaagg cgtgcaaccc atcgcctggg gagatgcact gcgcgaaagc gaatttaccc
    14581 gctgggttta tcagggattg tgtggtctgg agaaggagca gaaagatgcg ctttgatact
    14641 gtcattatgg gcggcggcct cgccggatta ctctgtggcc tgcaactgca aaaacacggc
    14701 ctgcgctgtg ccattgtcac gcgtggtcaa agcgcgctgc atttctcatc cggttcgctg
    14761 gatttgctga gccatctgcc tgatggtcaa ccggtgacag acattcacag tggactggaa
    14821 tctttgcgtc agcaggcacc agcccatcct tacagtcttc tcgaaccaca acgcgtgctc
    14881 gatctcgctt gccaggcgca ggcattgatc gctgaaagcg gtgcgcaatt gcagggcagc
    14941 gtggaactgg cccatcagcg cgttaccccg ctcggcactt tgcgtgcaac ctggttaagt
    15001 tcgccagaag ttcccgtctg gccgctaccc gcgaagaaga tatgtgtagt ggggataagc
    15061 gggctgatgg atttccaggc gcatcttgcg gcggcttcgt tgcgcgaact ggatctttcg
    15121 gttgaaaccg cagaaataga gctgccggaa ctggatgtcc tgcgcaataa cgccaccgaa
    15181 tttcgcgcgg tgaatatcgc ccgtttcctt gataatgaag aaaactggcc gctgtt
//
LOCUS       DAMZET010000101        14990 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.101, whole genome shotgun sequence.
ACCESSION   DAMZET010000101 DAMZET010000000
VERSION     DAMZET010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.101"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            375..1244
                     /gene="hexR"
                     /locus_tag="POB34_003130"
                     /gene_synonym="yebK"
     CDS             375..1244
                     /gene="hexR"
                     /locus_tag="POB34_003130"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HDI5920024.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            1372..2814
                     /gene="pyk"
                     /locus_tag="POB34_003131"
     CDS             1372..2814
                     /gene="pyk"
                     /locus_tag="POB34_003131"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDI5920025.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAARLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(2945..3916)
                     /gene="lpxM"
                     /locus_tag="POB34_003132"
                     /gene_synonym="msbB"
     CDS             complement(2945..3916)
                     /gene="lpxM"
                     /locus_tag="POB34_003132"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HDI5920026.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(4036..5358)
                     /gene="mepM"
                     /locus_tag="POB34_003133"
     CDS             complement(4036..5358)
                     /gene="mepM"
                     /locus_tag="POB34_003133"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HDI5920027.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(5374..6306)
                     /gene="znuA"
                     /locus_tag="POB34_003134"
     CDS             complement(5374..6306)
                     /gene="znuA"
                     /locus_tag="POB34_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HDI5920028.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            6385..7140
                     /gene="znuC"
                     /locus_tag="POB34_003135"
     CDS             6385..7140
                     /gene="znuC"
                     /locus_tag="POB34_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HDI5920029.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            7137..7922
                     /gene="znuB"
                     /locus_tag="POB34_003136"
     CDS             7137..7922
                     /gene="znuB"
                     /locus_tag="POB34_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HDI5920030.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(8069..9079)
                     /gene="ruvB"
                     /locus_tag="POB34_003137"
     CDS             complement(8069..9079)
                     /gene="ruvB"
                     /locus_tag="POB34_003137"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDI5920031.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(9088..9699)
                     /gene="ruvA"
                     /locus_tag="POB34_003138"
     CDS             complement(9088..9699)
                     /gene="ruvA"
                     /locus_tag="POB34_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDI5920032.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(9838..9903)
                     /gene="yobI"
                     /locus_tag="POB34_003139"
     CDS             complement(9838..9903)
                     /gene="yobI"
                     /locus_tag="POB34_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HDI5920033.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            9974..10576
                     /locus_tag="POB34_003140"
     CDS             9974..10576
                     /locus_tag="POB34_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HDI5920034.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(10578..11099)
                     /gene="ruvC"
                     /locus_tag="POB34_003141"
     CDS             complement(10578..11099)
                     /gene="ruvC"
                     /locus_tag="POB34_003141"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDI5920035.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(11134..11874)
                     /locus_tag="POB34_003142"
     CDS             complement(11134..11874)
                     /locus_tag="POB34_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI5920036.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(11903..12355)
                     /gene="nudB"
                     /locus_tag="POB34_003143"
     CDS             complement(11903..12355)
                     /gene="nudB"
                     /locus_tag="POB34_003143"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HDI5920037.1"
                     /translation="MEDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(12473..14245)
                     /gene="aspS"
                     /locus_tag="POB34_003144"
     CDS             complement(12473..14245)
                     /gene="aspS"
                     /locus_tag="POB34_003144"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDI5920038.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAGIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            14555..>14990
                     /locus_tag="POB34_003145"
     CDS             14555..>14990
                     /locus_tag="POB34_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDI5920039.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWEL"
BASE COUNT         3560 a         3888 c         3709 g         3833 t
ORIGIN      
        1 ccaggtcaca ggcctgggct gtttgcgtta ccgccatgtc attctcctta agttaactaa
       61 cccggtactt aagccagggt atacttgtaa ttttcttacg gtgcactgta ctgcttttac
      121 gagcttgcga aaactgtaaa cgcttatcca cccgtgcgat tacggtaaaa gcgcgcaaag
      181 tgcggcaaaa ctgataaaaa aatcatcgtt ttccattggg taaaaatctg acactgatca
      241 tgttatgaaa aaaaataaca acttttttat ctgcttttgt cattaacaca gcacacaggc
      301 gtaatatctg acaaaactac atttcgattt ctttcagtgc ggaaatcgtc attacccgtg
      361 agtttcttta catcatgaat atgctggaaa aaatccagtc tcagctggaa catttgagca
      421 aatcagagcg caaagttgcc gaggtcattc tggcttcgcc cgataacgcg atccattcga
      481 gtattgctgc tctggcgctg gaagccaatg ttagcgaacc gacggtgaat cgtttctgtc
      541 gcagcatgga cacgcgcggt tttcctgatt ttaaacttca tctggcacaa agtctggcga
      601 atggcactcc ctatgttaat cgcaatgtca atgaagatga cagcgttgaa tcatacacag
      661 ggaaaatatt tgagtccgca atggcaacgc ttgatcatgt ccgtcattca ctggataaat
      721 ctgccatcaa ccgcgccgtc gacttgctca ctcaggcaaa aaaaatcgcc tttttcggat
      781 taggctcttc agccgccgtt gcccacgatg cgatgaataa gttctttcgt tttaatgttc
      841 cggtggtgta ctccgatgat atcgtgctgc aacgcatgag ttgtatgaat tgtagcgacg
      901 gagacgtggt ggtgctgatt tctcacactg gaagaacaaa aaatctggtc gagctggcgc
      961 agctggctcg cgaaaacgac gcaatggtga ttgccctcac ctctgcgggt accccgctcg
     1021 cccgggaagc aacgctggca attaccctcg acgtaccgga agatactgac atttatatgc
     1081 ccatggtttc tcgacttgca cagctgaccg tgatagatgt gctggcgaca ggatttactt
     1141 tgcgacgcgg tgcaaaattc agagataact tgaagcgggt caaggaagcg ctgaaggaat
     1201 cgcgttttga taagcagtta cttaatttaa gtgacgatcg ctaaaaacga ctgtcactgt
     1261 cctaatctta tacgacatcc gaatgagatt aatttatcgc catcgcggcg ttatttcatt
     1321 cggatttcat gttcaagcaa cacctggttg tttcagtcaa cggagtatta catgtccaga
     1381 aggcttcgca gaacaaaaat cgttaccacg ttaggcccag caacagatcg cgataataat
     1441 cttgaaaaag ttatcgcggc gggtgccaac gttgtacgta tgaacttttc tcacggctcg
     1501 cctgaagatc acaaaatgcg cgcggataaa gttcgtgaga ttgccgcaag actggggcgt
     1561 catgtggcta ttctgggtga cctccagggg cccaaaatcc gtgtatccac ctttaaagaa
     1621 ggcaaagttt tcctcaatat tggggataaa ttcctgctcg acgccaacct gggtaaaggt
     1681 gaaggcgaca aagaaaaagt cggtatcgac tacaaaggcc tgcctgctga cgttgtgcct
     1741 ggcgacatcc tgctgctgga cgatggtcgc gttcagttaa aagtactgga agttcagggc
     1801 atgaaagtgt tcaccgaagt gaccgtcggt ggtcccctct ccaacaataa aggtatcaac
     1861 aaacttggcg gcggtttgtc ggctgaagcg ctgaccgaaa aagacaaagc agacattaag
     1921 actgcggcgt tgattggcgt agattacctg gctgtctcct tcccacgctg cggcgaagat
     1981 ctgaactatg cccgtcgcct ggcacgcgat gcaggatgtg atgcgaaaat tgttgccaag
     2041 gttgaacgtg cggaagccgt ttgcagccag gatgcaatgg atgacattat tctcgcctct
     2101 gacgtggtaa tggttgcacg cggcgacctc ggtgtggaaa ttggcgaccc ggaactggtc
     2161 ggcattcaga aagcgttgat ccgtcgtgcg cgtcagctaa accgagcggt aatcacggcg
     2221 acccagatga tggagtcaat gattactaac ccgatgccga cgcgtgcaga agtcatggac
     2281 gtagcaaacg ccgttctgga tggtactgac gctgtgatgc tgtctgcaga aactgccgca
     2341 gggcagtatc cgtcagaaac tgttgcagcc atggcgcgcg tttgcctggg tgcggaaaaa
     2401 atcccgagca tcaacgtttc taaacaccgt ctggacgttc agttcgacaa tgtggaagaa
     2461 gctattgcca tgtcagcaat gtacgcagct aaccacctga aaggcgttac ggcgattatt
     2521 accatgaccg aatcgggtcg taccgcgctg atgacctccc gtatcagctc tggtctacca
     2581 attttcgcca tgtcgcgcca tgaacgtacg ctgaacctga ctgctctcta tcgtggcgtt
     2641 acgccggtgc actttgatag cgctaatgac ggcgtagcag ctgccagcga agcggttaat
     2701 ctgctgcgcg ataaaggtta cttgatgtct ggtgacctgg tgattgtcac ccagggcgac
     2761 gtgatgagta ccgtgggttc tactaatacc acgcgtattt taacggtaga gtaagtacgt
     2821 tgccggatgc ggcgaaaacg ccacatccgg cctacagttc aatgatagtt caacagattt
     2881 cgaatattct gaagcaaact tgaacttatc atcaggcgaa ggcctctcct cgcgagaggc
     2941 ttttttattt gatgggataa agatctttgc gcttatacgg ctggatttcg cccggtttgc
     3001 gagttttcag caattttagt atccaggtgt attgttctgg tcgcggacca acaaaaatct
     3061 cgacttcttc attcatccgc cgcgcaatcg tatgatcatc cgcctctaac agatcatcca
     3121 tcggtgggcg cacctgaatc gtcagacgat gcgtcttgcc atcataaatc ggaaacagcg
     3181 gtacaacgcg cgcacggcac actttcatca aacgaccaat cgcgggcaac gtcgctttat
     3241 aggtggcaaa gaaatccaca aattcgctgt gctctgggcc atgatcctga tcgggtaaat
     3301 aatatcccca gtacccctga cgtaccgact ggatgaatgg tttaataccg tcatttctcg
     3361 catgcagacg accgccaaag cgacgacgca ccgtgttcca gacataatca aaaaccgggt
     3421 tgccctgatt atggaacatc gctgccattt tctgcccttg cgaggccatc agcatggcag
     3481 gaatatcgac ggcccaaccg tgcggcacca gaaagataac tttctcgtta ttacgcctca
     3541 tctcttcgat gatctccagc ccttgccagt caacgcgcgg ctgaattttc tccggtccgc
     3601 gtattgccaa ctcagccatc attgccatcg cttgcggcgc ggtggcaaac atctcatcaa
     3661 caatcgcttc gcgttcagct tcactacgtt ctggaaagca gagcgacaga ttgattaacg
     3721 cacgacggcg tgagcttttt cccagtcgtc cggcaaaacg tcccagccgt gccagaatgg
     3781 gatcacggaa ctttggcggc gttaaagcga tacccgccat cgctgctacg cccagccatg
     3841 ctccccagta gcgcgggtgg cgaaaggatt tatcaaactc aggaatgtat tcgctattat
     3901 tttttttcgt ttccatgctt ttccagtttc ggataaggca aaaatcaatc tggtgatagt
     3961 gtagcggcgc aacttgcccc gcaccaaata aaaaaagccg gtactgactg cgtaccggct
     4021 gcgaatggat gttaattaat caaaccgtag ctgcggcaca atctctttgg cctgcgccag
     4081 gaattcgcga cgatcggagc cggtcagccc ttcggtacgc ggcagttttg ccgtcagcgg
     4141 gtttacggcc tgctggttta tccatacttc atagtgcaga tgcggcccgg ttgaacgtcc
     4201 ggtattaccg gaaagcgcga tacggtcgcc acgtttcacc ttctgtcccg gtttcaccag
     4261 aatcttgcgc aagtgcatat aacgcgtggt gtagctgcga ccatgacgaa tagccacata
     4321 ataacctgct gcgccactgc gtttggcaac caccacttca ccgtcaccca ctgaaagcac
     4381 tggcgtacct tgtggcatgg cgaaatcaac acctctgtgt ggtgcaacgc gacctgtcac
     4441 cggattagta cgacgcgggt taaagttaga cgagatacgg aactgtttcg ccgtcgggaa
     4501 tcgcaagaat cctttcgcca gaccagtacc gttacgatcg tagaatttgc catcctcagc
     4561 gcggatagcg taataatctt taccttctga acgcaaacgt acgcccagca gctggctttg
     4621 ctcacgttta ccatcaagca tttctcgaga cattaacacc gcaaattcat cgcctttttt
     4681 cagtttgcgg aaatccattt gccactgcat ggctttaatc actgcgctca cttcggcgct
     4741 ggttaaaccg gcgtttctgg cgctggcaac aaagctcccc ccgacggtac ctttcagcag
     4801 attgttaacc cactctcctt gctgcatttc gctggtcatt ttaaaaccgt tagcagcagt
     4861 acggtcatag gttcgggttt cacgacgaga cacttcccag gtgaggcgct gcagttcgcc
     4921 gtccgcggtt aatgtccagg agagttgttg accgattttc agattacgca attctttgtc
     4981 ggcagcagcc agttgggtga tatcacccat atcaatacca tactgattga gaatgctgct
     5041 tagcgtatcg ccagtggaaa caacatattc atgcacgccc gcttcaccgg cgattttgtc
     5101 atccagttcg tcctggggaa tggcttcatc ttcttgtgca gcttgatcaa tcggctcact
     5161 ggcttcaggt aagagcgaac gaatttcgtt ctgttccagc tcaatggttt tgacaattgg
     5221 cgtggcatca cggtgataaa cataaggccg ccagacagcg acggccagag taagaacggt
     5281 gagcgacccc aacataacgc ggtgtggtcg cggtaaatta ttaaacgcca gggcgacaga
     5341 gcgggctatc tgttgcacgt aatcacttcc tcattaatct cctttcaggc agctcgcata
     5401 ctggttggct aattgactca ggaattctga atagcttgtt ttacccagtt tgatattcgt
     5461 ccccagggga tccaacgttc ccatacgaac ggatgtccct cgtgcgacgc tctcaacgac
     5521 cgctggcctg aactgtggct cagcaaaaac gcaggttgct ttttgctcaa ccaactgtgt
     5581 tcttatttca tgtaaacgct gcgcgccagg ttgaatctca gggttaacgg taaaatgacc
     5641 aagcggtgtc agtccaaact gtttttcgaa atagccgtaa gcatcgtgaa aaacgaaata
     5701 acctttcccc ttgagcggcg cgagctcgtt accaacctgc gtttcggttg aggctaattg
     5761 tgcctcaaaa tccttcaggt tggcgtcaag tttggctcga ctttgcggca taagttccac
     5821 taattttcca tggattgcaa ccgctgtagc ccgcgctatc tctggggaaa gccaaagatg
     5881 catgttgaaa tcgccgtgat ggtgatcttc gtcacttttt tccgcgtggt cgtgatcatc
     5941 atcatcgccg tgaatacttt tcatcagcag cggtttcaca tcttctagct gtgcaatcgt
     6001 tacctgtttc gcttctggta atttacttac cggtttttgc ataaacgctt ccatctccgg
     6061 gccaacccaa acgactaagt ccgcgttctg taagcgtttt acatccgatg gacgcagtga
     6121 ataatcatgt tctgaagcgc cgtcaggcag taaaacctcc gtttctgtta caccatcagc
     6181 aatggcagaa gcgatgaacc caacgggttt aagcgaagcg acaacggcgg catctgcggc
     6241 ctgtgttgca cctccccaga gagcggcgga taatgctgcg aaaagaagcg tttttttatg
     6301 taacataatg cgaccaatca tcgtaatgaa tatgagaagt gtgatattat aacatttcat
     6361 gactactgca agactaaaat taacatgaca agtctggttt ccctggaaaa tgtctcggtt
     6421 tcttttggcc aacgccgcgt cctctctgat gtgtcgctgg aacttaaacc tggaaaaatt
     6481 ttgactttac ttgggccaaa tggcgcaggt aagtcgacac tggtacgggt agtgctcggg
     6541 ctagtaacac ccgatgaagg ggttatcaag cgtaatggaa aactgcgcat cggctatgta
     6601 ccgcagaagc tgtatctcga caccacgttg ccactgaccg taaaccgttt tttacgctta
     6661 cgccctggta cacataaaga agatattttg cctgcactga aacgtgtcca ggccgggcac
     6721 ctgattaacg caccgatgca aaagctctct ggtggcgaaa cgcagcgtgt actgttagcg
     6781 cgagcattgt taaatcgccc gcaattatta gtgctggatg aacccactca aggcgtggat
     6841 gtaaatggcc aggtggcgtt atatgacctt attgaccagt tgcgtcgcga actggattgt
     6901 ggcgttttaa tggtctctca cgatctgcat ctggtaatgg caaaaaccga tgaagtgctg
     6961 tgtctgaatc accacatttg ttgttccggc acaccggaag ttgtttccct gcatccggag
     7021 tttatttcaa tgtttggtcc tcgtggtgct gaacaactgg gtatctatcg ccatcatcat
     7081 aatcatcgtc acgatttaca gggacgaatt gttttgcgtc ggggaaatga tcgctcatga
     7141 ttgaattatt atttcccggt tggttagccg ggatcatgct cgcctgtgcc gcgggtccgc
     7201 tgggttcgtt tgtagtctgg cgtcgtatgt cttatttcgg tgatacgctg gctcatgcct
     7261 cattacttgg tgtcgcgttt ggtttgttgc tggacgtgaa tccattctat gcggtgattg
     7321 ccgttacgct gctgctggcg ggcggtctgg tatggctgga gaagcgtcca cagctggcga
     7381 tcgacacgtt attagggatt atggcgcaca gtgccctgtc gctgggcctg gtggtcgtta
     7441 gtctgatgtc taatattcgt gttgatttga tggcttacct gttcggtgat ttgctggcag
     7501 tgacgccaga agatctcatc tctattgcga ttggcgtggt catcgtggtg gctattttgt
     7561 tctggcaatg gcgcaatttg ctgtcgatga cgattagccc ggatctggcg tttgttgatg
     7621 gtgtgaaatt acagcgcgtg aaattgttgt tgatgctggt gacggcattg acgattggtg
     7681 tagcgatgaa atttgtcggt gcgttgatta ttacttcgtt gctgattatt cctgcagcta
     7741 ctgcgcgtcg ctttgcccgt acgccggaac agatggctgg tgtcgctgtt ttggtgggga
     7801 tggtggcagt aactggcggt ttaacctttt ccgcgtttta cgatacgccg gcgggtccgt
     7861 cggtggtgct atgtgcggca ctgttattta ttctcagtat gatgaaaaag caggccagct
     7921 aatctatcgc tgaacacatt tgtcggatgc ggcgcgagcg ccttatccga cctacggttc
     7981 gctatctctg gtaggcctgg taagacgcga atagcgtcgc atcaggcaca ctgccaatgt
     8041 cggatgcggt gcgagcgacc aatccgactt acggcatttc tggcggcgtt atgccaaagt
     8101 gattccacgc ccgcgtcgtc gccatacgcc cacgcggtgt acgctgcaaa aagccttgct
     8161 gaatcaaata aggttccagc acatcctcaa tggtttcacg ttcttcgcca atggctgccg
     8221 caaggttatc cagacctacc ggcccaccaa agaacttatc gattaccgcc agcaataact
     8281 tgcggtccat ataatcgaaa ccttcagcat cgacattcaa catatccaac gcctgagcag
     8341 cgatatctgc cgagatggtg ccatcgtgct tcacttcggc gaaatcacgc actcgacgca
     8401 gcagacggtt ggcaatgcgc ggcgtaccgc gagctcgacg agcaacttcc agcgcgccgt
     8461 catcactcat ctcaagcccc ataaagcgtg cgctgcgact gacgatatat tgcagatccg
     8521 gcacctgata aaactccaga cgctgcacaa taccaaaacg atcgcgcaac ggtgatgtca
     8581 gcgaacctgc gcgcgtggtt gcaccaatca gggtaaacgg cggcaaatca attttaatgg
     8641 agcgtgccgc cggaccttca ccaatcatga tatccagttg gtagtcttcc attgccggat
     8701 acaacacctc ttccaccact ggcgaaagac ggtggatctc atcaataaac aacacgtcat
     8761 gcggttcaag gttagtgagc atcgctgcca gatcgcccgc cttttccagc accggaccag
     8821 aagtcgtgcg taaattaacg cccatttcat tggcgacgat gttggcaagc gtagttttac
     8881 ccaaccccgg aggaccgaaa atcaacagat gatcgagggc atcgccgcgc agtttcgctg
     8941 ctttgatgaa aatctccatc tgtgaacgaa cctgcggctg accaacatac tcttccagta
     9001 atttagggcg aatggcgcga tctgccacat cttccggcaa agtggtaccg gcagaaatca
     9061 gacggtctgc ttcaatcatc ctttacctca taacgcggcg cgtagggctt cgcgaattaa
     9121 tgtttcactg ctggcgtcag ggcgagcgat tttgctcacc atgcggcttg cctcttgtgg
     9181 tttatagccc agcgccacca gcgcggcaac tgcttcttgt tcagcatcgt cggtcgccgg
     9241 gctggcaggt gatgtgagta ccaggtcggc ggctggcgta aagagatcgc catgcaaacc
     9301 tttaaatcgg tctttcattt caacaatcaa gcgttcggcg gtttttttgc caatacccgg
     9361 cagtttcacc agcgccccca cttcttcacg ctcaacggca ttaacgaact gctgcgctga
     9421 cattccggag aggatcgcca gcgccaactt cgggccgacg ccgttggttt tgatcaactc
     9481 tttgaacaat gtgcgctctt gtttattgtt aaaaccgtac agcagttgcg cgtcttcacg
     9541 caccacaaag tgggtgaaaa cgatcgcttc ctgacccgct tcagggagtt cataaaaaca
     9601 ggtcatcggc atatgcactt catagcctac gccgcccact tcaattaaca ccagcggggg
     9661 ttgtttttca atgatgatgc ctctgagtct gcctatcaca tgacgctcct gcgtaatgaa
     9721 tcaaagataa tgctgtatga taaaaaaatg ctggatagat atccagcgaa ggatgaagaa
     9781 aacttgcgag gtgtcccgat gatctgaaaa atggcagagt ataatttatt caacaggtta
     9841 tattggaagc aaatatttga atattacata ttcagtgaag aaatgcgtaa taaaaatata
     9901 cattgcgact cctgaaaaaa taaatttttt atgctattac gtatattcgt atcgatttca
     9961 atggaatgac aacgtgaata ttaattaccc tgctgaatat gaaattggag atatcgtctt
    10021 tacatgtata ggtgctgcct tatttggtca aatatcagct gcatcaaatt gctggagtaa
    10081 tcacgtcggg atcattatcg gtcataacgg tgaagacttc ctggttgcag aaagccgggt
    10141 tcccctttca accatcacca cgctatctcg ttttattaaa cgttcgtcta atcaacgcta
    10201 tgctataaag cgattagacg ctgggctgac agaacaacaa aaacaacgaa ttgttgaaca
    10261 ggttccttcc cggctacgca aactttacca cacgggtttt aaatacgaat cttcccgcca
    10321 gttctgttca aaatttgtct ttgatattta taaagaggcg ctatgtattc cggtgggtga
    10381 aatagagacg tttggacaac tgttaaatag caatccgaat gcaaaactca ctttctggaa
    10441 attctggttc ttaggttcta ttccgtggga gcgtaaaacc gtcacgccag ccagtttgtg
    10501 gcatcatccg ggtttggtgt tgattcacgc ggagggagtt gaaacgcctc agcctgaact
    10561 gaccgaggcg gtataactta acgcagtcgc cctctcgcca ggttcagccg cgattcgctc
    10621 atctgcatcg cattctgact gacgtgacaa tgggtaatcg cgatagctaa cgcatcggcg
    10681 gcatccgcct gtggattagc gggcagtttc agcaaggtgc ggaccatatg ctgcacctgg
    10741 cttttttcgg cactaccaat acctaccacc gtttgcttta cctgacgtgc cgcgtattca
    10801 aataccggca attcctgatt caccgccgca acaatcgcca cgccgcgcgc ctggcccagt
    10861 ttcagggctg agtcagcgtt cttcgccata aagacttgtt caatggcgaa ataatcaggc
    10921 tggaactggg tgatgatttc cgtcacgccc gcatagatga gcttcagacg agacggtaaa
    10981 tcatccactt tggtgcgtat gcatccgcta cccaggtagg acagttgcct gcctacctgg
    11041 cgaattacgc catagccggt cacgcgcgaa cccggatcaa tgccgagaat aatagccatc
    11101 acgcgtctcc gttttgctgt ttagcaggcc tcatcagaga gtcgctgcga cctcatcaga
    11161 gatttcaccg ttatggtaaa cttcctgcac gtcgtcgcaa tcttccagca tatcgatcag
    11221 acgcatcagt ttcggtgcgg tttctgcatc catatcagct ttggtggacg ggatcatgga
    11281 aacttccgcg ctgtctgctt tcagacctgc cgcttccaga gcgtcgcgca ctttacccat
    11341 ttcttcccat gcagtgtaga catcaatcgc gccgtcatca taggtcacaa cgtcttcagc
    11401 acctgcttcc agagctgctt ccatgatggt gtcttcatcg cctttctcga aggagatcac
    11461 gccttttttg ctgaacagat aggctacgga accatctgta ccgaggttac cgccacattt
    11521 gctgaatgca tgacgcactt cagcaacggt acggttgcgg ttgtcagaca gacattcaat
    11581 catgattgcc gtgccgccag gaccgtaacc ttcgtagatg atggtttcca tgtttgcatc
    11641 atcatcaccg cccacaccac gtgcaattgc gcggttcagt gtgtcacggg tcatgttgtt
    11701 agacagtgct ttatccaccg ccgcacgcag acgcgggtta gcgtccggat cgccaccgcc
    11761 cagcttagcc gcggttacca gctcacgaat gattttagtg aagattttac cgcgcttagc
    11821 atcctgcgca gctttacgat gtctggtgtt ggcccattta ctatgacctg ccataaaaat
    11881 atctccagat agccctgcct gttcaggcag cgttaattac aaactgttca atcgcctgcc
    11941 ggttgctcca ggacttagtg agcgccgccg cagcaggcgc atcaagccac ttgtaagcca
    12001 gatgttcagt gaaaacgatc tgccgctcgt gcggaagcgc aagacagaac catgattccg
    12061 tattacgcgt cacgcccggc gcatagcgat gacgtaaatg tgaaaaaatt tcaaactcta
    12121 ccgtgcgctg acagtcaatt aaggtcagtt gttcagcgac aacatcaatg gtgacctctt
    12181 cctttacttc gcgcatggca gcttgcggcg cggtttcacc ctcttccacg ctgccggtta
    12241 ccgactgcca gaaatcggga tcgtcacgcc gctgcaacat cagcacccgt ttcgtatctt
    12301 gtgcatagat gaccactaag atcgaaacgg gacgcttata cactttatcc tccacgctgc
    12361 ctcttcgttg actgccttcg ctcatcccat tcacatagtt atctatgctc atgggagttc
    12421 actcagttgc cgccttgatg caacgcgaat gatttcgtgt atttgagtca tatcagttat
    12481 tctcagcctt cttcacaacc tgaatgctca gctcagccag tgcagtcggg ttagcaaagc
    12541 tcggtgcttc agtcatcaga cacgctgccg ccgtggtttt cgggaaggcg ataacgtcac
    12601 ggatattgtc ggtgccggtc agcagcatgg tcagacggtc aagaccgaat gccagacctg
    12661 cgtgcggcgg agtaccgtat ttcagagcgt cgagcaggaa gccgaatttc tcgcgctgtt
    12721 cctcttcgtt gatacccaga ataccaaaca ccgtctgctg catatcacca ttatggatac
    12781 gtactgaacc accgcccact tcgtaaccat tgatgaccat atcgtaagcg ttcgccaccg
    12841 cattttccgg tgcagctttc agttctgcag cagtcatgtc tttcggtgag gtgaacggat
    12901 ggtgcattgc cgtcaggccg ccttcaccgt cgtcttcaaa catcgggaag tcgataaccc
    12961 acagcggtgc ccatttgctt tcgtcggtca gaccaaggtc tttacccact ttcaggcgca
    13021 gtgcgcccat cgcgtcggca acaattttct tgttgtcggc accgaagaaa atcatatcgc
    13081 catcttgcgc ggcagtacga tccaggatgg cttcgatgat tcctgcatta aggaacttcg
    13141 ctaccgggct gttgatacct tccagacctt tcgcgcgttc gttaactttg atgtaagcca
    13201 gacctttcgc gccgtagatt ttaacgaagt taccgtattc gtcgatctgc ttacgggtca
    13261 gcgatgcgcc gcccggaacg cgcagagcgg caacgcgacc tttcggatcg ttcgccggac
    13321 ctgcaaatac tgcaaactca acagatttca gcagatcagc aacgtcggtc agttccatcg
    13381 ggttacgcag atccggttta tcagaaccat aacggcgttc tgcttccgca aaggtcatta
    13441 ccgggaaatc gcccagatcc acgcccttca cttccagcca caaatgacgc accagcgctt
    13501 ccatcacttc acgcacttgc ggcgcggtca tgaaagaagt ttccacatcg atctgagtaa
    13561 attcaggctg acgatcagcg cgcaggtctt cgtcacggaa gcatttaacg atctgatagt
    13621 aacggtcaaa accggacatc atcagcagct gtttgaacaa ctgtggggat tgcggcagtg
    13681 cgtagaattt acctttgtgc acacgagaag gcaccaggta gtcacgcgcg ccttccggcg
    13741 tggctttggt cagcatcgga gtttcgatgt cgaggaagcc gtggtcatcc ataaaacggc
    13801 gcaccaggct ggtgatttta gcgcgggttt tcaggcgctg agccatttcc ggacgacgca
    13861 ggtcgaggta gcggtatttc agacgcgctt cttcggtgtt gacgtggtta gagtcaagcg
    13921 gcagaacatc tgcgcggttg atgatagtca gcgaggacgc cagcacttcg atttcgccgg
    13981 tcgccatatc gcggttaata tttttttcgt cacgcgcacg tacggtgccc gtgacctgaa
    14041 tgcagaactc attacgcagt tcagaggcca gctttaacgc gtccgcacga tccggatcga
    14101 aaaatacctg cacgatacct tcgcggtcgc gcatatcgat gaagatcagg ctaccaagat
    14161 cacgacgacg gttgacccaa ccacacagag tcacctgctg ccccacgtgg gacaaacgga
    14221 gctgtccaca atattctgta cgcatgagat atcccttaac ttagctgccg gcggatgccc
    14281 cctgctgcgc aggtgaccaa gtcgcagcgt tagctgtatg tcacaactga atgaaaaaag
    14341 gcggctatta tactggaaat tctgccgcac cgtaagagcc tggcccgcgc tggaacgcct
    14401 cgttaccact ttatatcggg cctgaaatca gactctacgc cagtttgcta taaaggtgtt
    14461 gcccgaactc ataaaaatta acaaaatttg tcgttccgcc atcggctaat cgcattaagg
    14521 tgtgaggcac gattttgttt tttcaggagt cattatgctt gaacttaatg ctaaaaccac
    14581 tgcgctggtg gtgatcgatt tacaagaagg catcttacct tttgccggtg gtccacatac
    14641 tgccgatgag gtagttaatc gcgccgggaa gctggcggcg aaatttcgcg ccagcggtca
    14701 gcccgtgttt ctggtgcgcg ttggctggtc tgccgattac gccgaagcat taaaacagcc
    14761 ggtcgatgcg ccctcaccgg ctaaagtgtt acccgaaaac tggtggcaac atcctgctgc
    14821 attaggtgca actgacagtg atatcgaaat catcaaacgt cagtggggtg cgttttacgg
    14881 tacggatctg gagttgcaac tgcgccgccg aggtatcgat acaatagtgt tatgtgggat
    14941 ctcgaccaat atcggtgttg aatccaccgc ccgcaatgcc tgggaacttg
//
LOCUS       DAMZET010000102        14952 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.102, whole genome shotgun sequence.
ACCESSION   DAMZET010000102 DAMZET010000000
VERSION     DAMZET010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.102"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..284)
                     /locus_tag="POB34_003146"
     CDS             complement(<1..284)
                     /locus_tag="POB34_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920040.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAANYDTWGYHDNTTTDLV
                     WPYDTDNDGIADAGGMDPMGSDGNYVTYNGFVYYNNANGDFDTVFKGDTV"
     gene            808..1782
                     /gene="hemB"
                     /locus_tag="POB34_003147"
     CDS             808..1782
                     /gene="hemB"
                     /locus_tag="POB34_003147"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDI5920041.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1888..2739)
                     /gene="tauD"
                     /locus_tag="POB34_003148"
     CDS             complement(1888..2739)
                     /gene="tauD"
                     /locus_tag="POB34_003148"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HDI5920042.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2736..3563)
                     /gene="tauC"
                     /locus_tag="POB34_003149"
     CDS             complement(2736..3563)
                     /gene="tauC"
                     /locus_tag="POB34_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HDI5920043.1"
                     /translation="MSVLINEKLHSHRLKWRWPLSRQVTLSIGTLAVLLTVWWAVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(3560..4327)
                     /gene="tauB"
                     /locus_tag="POB34_003150"
     CDS             complement(3560..4327)
                     /gene="tauB"
                     /locus_tag="POB34_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HDI5920044.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSILLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLSLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(4340..5302)
                     /gene="tauA"
                     /locus_tag="POB34_003151"
     CDS             complement(4340..5302)
                     /gene="tauA"
                     /locus_tag="POB34_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI5920045.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            5918..6589
                     /locus_tag="POB34_003152"
     CDS             5918..6589
                     /locus_tag="POB34_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDI5920046.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            6599..7795
                     /locus_tag="POB34_003153"
     CDS             6599..7795
                     /locus_tag="POB34_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDI5920047.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYSQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            7773..8354
                     /locus_tag="POB34_003154"
     CDS             7773..8354
                     /locus_tag="POB34_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDI5920048.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            8356..9129
                     /locus_tag="POB34_003155"
     CDS             8356..9129
                     /locus_tag="POB34_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HDI5920049.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            9314..9589
                     /gene="frmR"
                     /locus_tag="POB34_003156"
     CDS             9314..9589
                     /gene="frmR"
                     /locus_tag="POB34_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HDI5920050.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            9624..10733
                     /gene="frmA"
                     /locus_tag="POB34_003157"
     CDS             9624..10733
                     /gene="frmA"
                     /locus_tag="POB34_003157"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HDI5920051.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDVPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            10826..11659
                     /gene="fghA"
                     /locus_tag="POB34_003158"
     CDS             10826..11659
                     /gene="fghA"
                     /locus_tag="POB34_003158"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDI5920052.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(11784..12323)
                     /locus_tag="POB34_003159"
     CDS             complement(11784..12323)
                     /locus_tag="POB34_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HDI5920053.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(12425..13636)
                     /gene="mhpT"
                     /locus_tag="POB34_003160"
     CDS             complement(12425..13636)
                     /gene="mhpT"
                     /locus_tag="POB34_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HDI5920054.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(13812..14825)
                     /gene="dmpG"
                     /locus_tag="POB34_003161"
     CDS             complement(13812..14825)
                     /gene="dmpG"
                     /locus_tag="POB34_003161"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HDI5920055.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADMVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(14822..>14952)
                     /locus_tag="POB34_003162"
     CDS             complement(14822..>14952)
                     /locus_tag="POB34_003162"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308433.1"
                     /note="catalyzes the formation of acetyl-CoA from
                     acetalaldehyde; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HDI5920056.1"
                     /translation="EVEGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
BASE COUNT         3684 a         3991 c         3654 g         3623 t
ORIGIN      
        1 ttaacggtat cgcctttaaa gacggtatcg aaatcgccat tggcattgtt atagtaaaca
       61 aaaccattgt aagttacgta gttaccatct gaacccatcg ggtccatgcc gccagcatcg
      121 gcaattccat cattgtcggt gtcatacggc caaactaaat cggtcgtggt gttgtcatga
      181 tacccccagg tatcatagtt tgctgcatta gcctgagagg tgatagccag agtgcaagcc
      241 aatgctaatt gtgatactac aagtttcttt ttccaggagt gcatttgtca tccctcctca
      301 gggacgtaat aagttgatcc atcaattatc aatgcataga aaacatatta tgtttcgcgc
      361 taacgattat gctgcgttac agacgaatgg ttcaattatt ctttccccta agtccgaatg
      421 tggacatata taatttatgg agaaaaacac gcatcaaatt gataaaaggg cgatatttca
      481 acatgttgca gataatgtta tttttgatac atacgcgtta taaatatcaa aaacacaaac
      541 cagataaata tcttatcaat atacaacaaa gtttgaatta ttcaatttat agcagcacaa
      601 ccaaacgcca aaaaataata accaagagct ataaataaag attaaaaata ttttcagtga
      661 atatgaaatt attgctattt ctattacaaa gatgtcctgg tctctctttc attctcttct
      721 tgtcaacatc acgacaactt tcgtaaaaca ctcctgcccc gcttcaggta tactgtgcac
      781 cttaattcca caaacatcag gcagaccatg acagacttaa tccaacgccc tcgtcgcctg
      841 cgcaaatctc ctgcgctgcg cgctatgttt gaagagacaa cacttagcct taacgacctg
      901 gtgttgccga tctttgttga agaagaaatt gacgactaca aagccgttga agccatgcca
      961 ggcgtgatgc gcattccaga gaaacatctg gcacgcgaaa ttgaacgcat cgccaacgcc
     1021 ggtatccgtt ccgtaatgac ttttggcatc tctcaccata ccgatgaaac cggcagcgac
     1081 gcctggcggg aagatggact ggtggcgcgt atgtcgcgca tctgcaagca gactgtgcca
     1141 gaaatgatcg tcatgtcaga cacttgtttc tgtgaataca cttctcacgg tcactgcggt
     1201 gtgctgtgcg agcatggcgt cgacaacgac gcgactctgg aaaatttagg caagcaagcc
     1261 gtggttgcag ctgctgcagg cgcagacttc atcgcccctt ccgccgcgat ggacggccag
     1321 gtacaggcga ttcgtcaggc gctggacgct gcaggcttta aagatacggc gattatgtcg
     1381 tattcgacca agttcgcctc ttccttttat ggcccgttcc gtgaagctgc cggaagcgca
     1441 ttaaaaggcg accgcaaaag ctatcagatg aacccaatga accgtcgcga ggcgattcgt
     1501 gagtcactgc tggatgaagc ccagggcgca gactgcctga tggttaaacc tgctggagcg
     1561 tacctcgaca tcgtgcgtga gctgcgtgaa cgtactgaat tgccgattgg cgcgtatcag
     1621 gtgagcggtg agtacgcgat gattaagttc gccgcgctgg cgggtgctat agatgaagag
     1681 aaagtcgtgc tcgaaagctt aggttcaatt aagcgtgcgg gtgcggatct gattttcagc
     1741 tactttgcgc tggatttggc tgagaagaag attctgcgtt aatttttttc ctctcagaaa
     1801 gggggagtta acctgttgaa agtacggtga acgccccctc accctaaccc tctccccaag
     1861 ggggcgaggg aaccgtccgc actcgtatca ccccgcccga taaaacggtt tatccccaag
     1921 aatcgtcgcc cgatgcataa tccgccgctg tggcaggtaa tcggcattgg catagtgctg
     1981 agtcacgcgg ttatcccaaa tcgcaatatc attcggttgc cagcgccaac gcacctgaaa
     2041 ctccggttta gtgatatggg cgaataaaaa gcctaacaag gcttcgctct ctttctcgct
     2101 gacatcgaca attcgcgtag taaagccttc attcacaaac agcgcctgtt taccgctcac
     2161 cggatgcgtt cgcaccaccg gatgtagcaa cggaggattt ttcgcgaccg cctcgcgcca
     2221 gcgttgatgt tcctcctcgg ttttgcggta tttgtattcc gggaacgatt tacggaaatc
     2281 atgctccgca cgcagcccgc tcagcagctg gcggaaggga acagagagcg cctcataggc
     2341 cgcaataccg ctggtccaga gcgtatcacc gccggtcgaa ggtaattctt tagctgccag
     2401 aatcgcccct gcgggtggcg tttcaataaa tgtcacatcg gtatgccagt tgtcgttatc
     2461 tggcggatta tcgttatggg tatccagcac gatgatctcg tcaacccctt cggcatgagg
     2521 gtaaacaggg tgaatatgca gttcgccaaa acgctgggcc agcgcgcgtt gctgctgcgg
     2581 cgtaatggct tgatcgcgca gaaacaccac ctgatggcgc agcaccgcat ggtaaagctg
     2641 ttcaaactga ttatcgctta acgggcgcgt caggtcggca cccgaaattt gtgcgccgat
     2701 atacggcccc agcggggtaa tgctcagacg ttcactcatt gtacttctcc atgccagggc
     2761 gtcagacggc gctgtaacgc gcgcaaaccc agttctaaaa gaaaggcgat aatcgcaatc
     2821 accgcgattc ccgccagcac cacgtcagtc gcgagaaatt cacccgctga ctgaaccata
     2881 aatcctaaac cgcgcgtcgc ggcaatcagc tccgccgcta ccagcgtaga ccagcccaca
     2941 cccagaccaa tgcgtaatcc ggtgaggatt tccggcagcg cgccgggcaa aatgacaaac
     3001 cacagcacct gcgcacggct ggcacccagc gactgggcgg cacgaatgcg aacctgctgc
     3061 gcgcttttca cccccgccag cgccgacatc gccaccggtg caaaaatcgc taaatagatc
     3121 agtaagatct tcgaggtttc accaatacca aaccagatca ccatcagcgg caaataagcc
     3181 agcggcggca ccggacgata aagttcgatt accggatcca gaatgccgcg caccgtcggg
     3241 cttagcccca tggcgatccc gaccggaata ccgatcaaca ccgccgcgag tagcgccagc
     3301 acaatgcgcg tcaggctggc tgccagatgc tgccacagcg tggcgtccat aaatccctgc
     3361 ggcccggcaa tggtgagcag ttttgccagt acctgttgcg gtggcggcaa aaacagcggg
     3421 ctaatcagtt gcagcgctgc gactgcccac catacagtaa gtaaaaccgc taacgtgcca
     3481 atgcttaagg tcacctgacg cgagagcggc cagcgccatt taagccgatg cgaatgcagt
     3541 ttttcattaa tgagcacact catgagaacg cctcccgctg ctcaaatacg cggcttaaaa
     3601 catattcgcg catggcgatg aattgtggat cggacttgat gctgcggctc gactctcccg
     3661 caacaaagcg gcgagcaaag ttgagcgaca gccgctccag cacacggcca gggccggatg
     3721 aaagcagaac cagttcagtc gccataaaca ccgcttcttc tatatcgtgg gtaatcaaca
     3781 gcacctgctt gcccgtctcc tgccagagtt tcagcagcag ggtttgcatc tggtcgcggg
     3841 tgaaggcgtc cagcgcacca aacggttcgt cgagtaataa cagctgggga ttcgccgcca
     3901 gcgcacgggc aatccccacc cgctgacgtt gaccaccgga aagctgccag atgtagcgtt
     3961 tttctgcgcc ttccagcccc acttttttca gcatctggtg cgcgatttcc agtcgctgca
     4021 ttttctctat acctgccaat tgcaggccga acgccacgtt gtcctgtaca ttgcgccacg
     4081 gtagtagccc ttcattctga aaaactacgc cacgctctgc tcccggtccc tcaatacgct
     4141 tacccgccag tagaatgctg ccatgctgat aaggcacaaa accggcaatc agattcagca
     4201 acgtggtttt accgcagccg gacggcccca gcaccaccag tagctcgccg ctttccagcg
     4261 tcaggttgat atcttccagt gccggtttgc cgccataatc ggcgtaaaga tgagagattt
     4321 gcagcatctg cgcctccttt tattgcacga agcgcgaggt aacgtactgg ctgtaatcat
     4381 tcgctacagc cgggaccttg ccctgctctt tcaaaaactg cgcggtgtcg atgatcgctt
     4441 tgttcaccgg tccggtcagt tctgccgttt gttgctgcgg cgtcagatag gtattcccct
     4501 tcaccagccc cggaacgtca ccttcaggca cgccgcttaa acgcgccagt ttgctgatgt
     4561 tttccggctg tttcagccac acgtctgggt tagcaatgta cggttgctga gcatcgatgg
     4621 cgcttttagc gaacgctttc acgacctcag gatgtttctc ggcaaaatct ttgcgcacca
     4681 cccagacgtc cagcgttggc gcgccccact gcccgacctg ttcagaatcg gtcaacacct
     4741 tgccgtcttt ttccagggcg ttaaccgccg gtgcccagac ataagcacca tcaatatctc
     4801 cccgctgcca ggcagcgata atcgcgggcg gctgcaggtt cacaatctcc acttgcccgg
     4861 gtttaatgcc ccagtgtttc agcgccgcca gcaggctgta gtgggtggtg gagataaacg
     4921 gtacggcgat gcgtttgcca atcagatctt ccggtttgct gatagttttc tttaccacca
     4981 gcgcttcgga gttacccagt tttgacgcca gcaagaagac ttcaatcggc acctgttggc
     5041 tggctgcaac cgctaacggg ctggaaccga ggttgccgat ttgcacatcg cctgaagcca
     5101 gcgcccgcac gatgctggct ccgctgtcaa acttacgcca gtccacgctc gccccgctct
     5161 ctttagcaaa ggtgttgtcg gcctgagcca ctttcgccgg ttcggctgag gtttgatagg
     5221 ccacggtgac gttcaccgcc tgtgcctgaa aagcgatgaa tgccagtgcg gctagaagtg
     5281 tgttacgcga tgaaattgcc atgattgtct gctcccctgt cttgttatgg gagcagtatt
     5341 caggaataaa aacattcatt aaaagaatta gtcgttatcg cacagatgat tttattctta
     5401 gcaaaaaaac ggctaaggaa agattaaata tgcaattact ccgttctaac aattgggaaa
     5461 attactacac ttcattttag tgatgcataa acaaccacat tacgagattt attacttcat
     5521 tatttctatc aatatcaata catataataa aactattata ttcgcattgt atatatcact
     5581 gtatcattct caatcactcg catattacat tcacataatg tatataaaca atgcttgttc
     5641 acagaacata ttttttaaat gcaattaatt gacaaactaa atcacatcta aatacaatgt
     5701 ataaaacaaa ataactatac ataccaaaca tacaaacact taacatatga attgcataga
     5761 aacattaccc agtcgaacct ccgggtctga aagcccttgc ttttcagtta ttagcaacat
     5821 ttaacattta taaattaaca tctggctgcg ccagagatta cgtctctgag tgcatcgttg
     5881 tgcttgtagt ataataaata aaatggagtg tcattaagtg gataaggttt tagattcagc
     5941 cctcctttct tcggcaaata aaagaaaggg tatccttgct attggcgcac atcctgacga
     6001 tatagaatta ggctgtggcg catcgcttgc tcgtcttgcg caaaaaggaa tttatatcgc
     6061 agccgtggtg atgactaccg gcaactctgg cacagatgga ataatcgatc gccatgaaga
     6121 atcgcgcaac gccctaaaga tattagggtg ccaccaaact attcatctta attttgctga
     6181 cacccgcgct catttacagc tcaatgatat gatttccgcc ctggaagaca tcattaaaaa
     6241 tcaaattcct tctgatgttg aaatcatgcg ggtatatacc atgcatgatg ccgaccgcca
     6301 tcaggatcat ctcgctgttt atcaagcttc aatggttgcc tgccgcacta ttccacaaat
     6361 tctcggctac gaaaccccaa gtacctggct ttcatttatg cctcaggttt ttgaatccgt
     6421 taaagaagaa tatttcacag tcaaacttgc tgcattaaaa aaacataaaa gccaggaacg
     6481 acgcgattat atgcgtcatg atcgcctgcg tgcagttgca caatttcgcg ggcaacaggt
     6541 caatagcgat ctgggtgaag gctttgtaat tcataaaatg attctttgag caaattgcat
     6601 gaaaacctgg atatttatct gtatgtccat agcaatgttg ctatggtttt taagtacgct
     6661 aagacgtaaa cccagtcaaa agaaaggctg tattgacgcc attatacctg cgtataacga
     6721 aggcccgtgt ctggcgcagt cactggataa tctactgcgt aacccttatt tttgccgggt
     6781 aatttgcgtt aacgacggct ccacggacaa taccgaagcg gtcatggcgg aagtcaaacg
     6841 caaatggggc gaccgctttg ttgccgtcac gcaaaaaaat accggtaaag gtggtgcgct
     6901 gatgaatggc ctcaattatg ccacctgcga ccaggttttt ttaagtgatg ccgacaccta
     6961 tgttccgccc gatcaagacg gaatgggcta tatgctggca gaaattgagc gcggtgctga
     7021 tgccgtaggc ggcattccct ctactgcgtt gaaaggcgcg gggctgttac cgcacatccg
     7081 cgcgaccgta aagttgccga tgattgttat gaagcgcacg ctacagcagc tcctgggcgg
     7141 cgcaccgttt attatcagcg gtgcctgcgg aatgttccgc actgatgtat tgcgtaagtt
     7201 cggtttctca gatcgtacta aagtcgaaga ccttgatctc acctggacat tagtggcaaa
     7261 cggctaccgt attcggcagg cgaatcgctg catcgtatac tcacaggaat gcaacagccc
     7321 gcgtgaggag tggcgtcgct ggcggcgttg gattgtggga tacgcggtct gtatgcgcct
     7381 gcataaaaga cttttattta gccgcttcgg tatcttcagt atatttccta tgctgttggt
     7441 tgtgatttat ggcgttggga tttatctcac tacctggttt aatgaattca tcaccaccgg
     7501 gccgcatggt gtggtgttgg caatgtttcc gcttatctgg atcggcgtag tttgtgttat
     7561 tggtgctttt agcgcctggt ttcatcgttg ctggttgttg gtgcctttag cgccgctttc
     7621 cgttgtgtat gtattattag cttatgccat ctggattatt tatggactta ttgccttttt
     7681 tactggacgc gaacctcagc gcgacaaacc cacccgctat tccgcactgg tggaagcgtc
     7741 aaccgcttat tcccaacctt ctgtcacagg aactgaaaaa ctatctgaag cttaatgtta
     7801 aagagaaaaa tattcagatt gcagaccagg taattattga tgaaactgca ggtgaagttg
     7861 ttatcggcgc gaatacccgt atttgtcatg gtgccgttat tcagggtccg gtagtgattg
     7921 gcgcaaactg cctgataggt aattatgcgt ttattcgtcc tggcacaata atcagcaatg
     7981 gtgtaaaaat tggttttgcc accgaaatta aaaatgcggt tattgaagcg gaagcaacga
     8041 ttggtccgca atgttttatt gccgactcgg tagttgcaaa ccaggcatat ttgggcgcac
     8101 aagtacgtac cagtaatcat cgtctggatg aacagcctgt gtctgttcga actccagagg
     8161 gaattatcgc taccggatgc gataaattag gttgttatat cgggcagcgt tcacgccttg
     8221 gcgtacaagt tataattttg cctgggcgaa ttatttcccc gaacacgcaa cttggcccgc
     8281 gcgtgattgt agaacgtaat ttacctgccg gaacttattc actccgacaa gaacttatcc
     8341 gtacaggaga ttaaaatgat aaaacggacg ttattagcgg cggccatttt tagcgcattg
     8401 cccgcttatg ccgggttaac ttccattacc gcgggctacg attttaccga ttattctggc
     8461 gatcatggca accgtaattt agcgtatgct gaactggtgg cgaaagttga aaacgcaacg
     8521 ctgcttttta atctttccca ggggcgtcgt gattatgaaa ctgaacattt caatgccact
     8581 cgcggtcagg gtgcggtctg gtataaatgg aataactggc tgacaacccg aacgggtatt
     8641 gcctttgcgg ataatacgcc ggtctttgcc cgccaggatt ttcgtcagga tattaacctg
     8701 gccctgttgc caaaaacgct tttcacgacc ggttatcgct acactaaata ttacgatgat
     8761 gtcgaagtcg atgcctggca aggcggcgta tcactctata ctggcccggt gatcaccagc
     8821 taccgctata cccattatga ctccagcgat gcaggtggta gttatagcaa tatgatttcc
     8881 gtgcgtctga atgacccgcg cggcactggt tatacgcaac tatggctaag ccgcggaaca
     8941 ggcgcttaca cctatgactg gacgccagaa acacgctacg gcagcatgaa gagcgtcagt
     9001 ttgcaacgta ttcaaccgct gactgagcaa cttaatctcg ggctgacggc aggtaaagtg
     9061 tggtacgaca ccccaaccga tgattataac ggtctgcaac ttgcagccca tctgacctgg
     9121 aaattctgat tccttctgcc gcccgctatc cggggcggcc ttccctgccg attagccccc
     9181 cctttcctct ttgttttccg accacattca ccggataaat tttattctcc agtgttatat
     9241 actatagggg ggtatgcatt gacatatagc atacccccct atagtatatt gcgtgcagat
     9301 gatgaggtgc gaaatgccca gtactccgga agagaagaaa aaggtcctta ctcgagttcg
     9361 tcgtattcga gggcagattg atgctctgga acggtcgctg gagggtgatg ccgaatgccg
     9421 tgccatactc caacagatcg ctgccgttcg gggcgcggct aacgggctga tggcagaagt
     9481 gcttgaaagc catatccggg aaacgtttga ccgaaatgac tgctacagcc gcgaagtcag
     9541 ccaatccgtt gacgacacta ttgaactggt tcgagcctat cttaaatagc tgaatctatt
     9601 accatattga ggaagagcga gagatgaaat cacgtgctgc cgttgcattt gctcccggta
     9661 aaccgctgga aatcgttgaa attgacgttg caccaccgaa aaaaggtgaa gtgctgatta
     9721 aagtcaccca taccggcgtt tgccataccg acgcatttac cctctccggg gatgtcccgg
     9781 aaggtgtatt cccggtggtt ctcggtcacg aaggggccgg cgttgtggtt gaagtcggtg
     9841 aaggcgtaac cagcgtcaaa cctggcgacc atgtgatccc gctttacacc gcggagtgcg
     9901 gcgagtgtga gttctgtcgt tctggcaaaa ctaacctctg tgttgcggtt cgcgaaaccc
     9961 agggtaaagg cttgatgcca gacggcacca cccgtttttc ttacaacggg cagccgcttt
    10021 atcactacat ggggtgctct acattcagtg aatacaccgt ggtcgcggaa gtgtctctgg
    10081 ccaaaattaa tccagaagca aaccatgaac acgtctgtct gctgggctgt ggcgtgacca
    10141 ccggtattgg cgcggtgcac aacacagcta aagtccagcc aggtgattct gttgccgtgt
    10201 ttggtcttgg cgcgattggt ctggcagtgg ttcagggcgc gcgtcaggcg aaagcgggac
    10261 ggattatcgc tatcgatacc aacccgaaga aattcgatct ggcgcgtcgc ttcggtgcta
    10321 ccgactgcat taacccgaat gactacgaca aaccgattaa agatgtcctg ctggatatca
    10381 acaaatgggg tatcgaccat acctttgaat gcatcggtaa cgtcaacgtg atgcgtgcgg
    10441 cgctggaaag tgcgcaccgc ggctggggtc agtcggtgat catcggggtg gcaggttctg
    10501 gtcaggaaat ctccacccgt ccattccagt tggtcactgg tcgcgtatgg aaaggttccg
    10561 cgtttggcgg cgtgaaaggt cgttcccagt taccgggtat ggttgaagat gcgatgaaag
    10621 gtgatatcga tctggaaccg tttgtcacgc ataccatgag ccttgatgaa attaatgacg
    10681 ccttcgacct gatgcatgaa ggcaaatcca ttcgaaccgt aattcgttac tgatttcccg
    10741 caggtatacc ccgtccactt cagacggggt tcttaatact ctccccgggc agccgtccgg
    10801 gggattaacc gtgagataat gactgatgga actcattgaa aaacatgcca gctttggcgg
    10861 ctggcaaaat gtgtatcggc attattccca atcactgaaa tgtgaaatga atgtcggcgt
    10921 ctatctccca ccaaaagccg cgaatgaaaa attgccggtg ctgtactggc tttcgggcct
    10981 gacctgcaac gagcagaatt tcattactaa atcggggatg cagcgttacg cggctgagca
    11041 caacattatt gttgttgcgc cggacaccag tccgcgaggc agtcatgtcg cagatgccga
    11101 ccgttacgat ctcgggcaag gtgccgggtt ttacctgaac gcaacgcaag cgccgtggaa
    11161 tgaacattac aaaatgtatg actatatccg caacgagctg ccggatttag tgatgcaaca
    11221 ttttccggca acgaccagaa agtctatctc tggtcattct atgggcgggc tgggcgcgct
    11281 ggtgctggcg ttacgtaacc cagatgaata tgtcagcgtc tcggcgtttt cgcccattgt
    11341 ctccccatcg caagtaccgt ggggacagca agcctttgcc gcatatcttg gtgaaaataa
    11401 agatgcctgg ttggattacg atccggtgag tcttatttca caaggtcaac gcgttgcgga
    11461 aatcatggtt gatcaggggt tgagtgatga tttttacgcg gaacagctgc ggactccaaa
    11521 tcttgaaaag atctgccagg agatgaatat caagacgtta atccgttatc acgagggtta
    11581 cgaccacagc tattattttg tctccagttt tattggcgag catattgcct accacgccaa
    11641 taaactgaat atgcgttgat aatagtgcac gactgccgga tgcggcgtga acgccttatc
    11701 cgcctacatt tcgcccgcag gccgtaggca tgataagacg cgcatagcgt cgcatcaggc
    11761 atctgcgcac gacttatcta tgtttaccac atcaaatcat caggcacttt gaagtcggca
    11821 tacggatcgt cttcatcctg ctcttcggca ctgagcgcgc tgtgcaacac tatactgctg
    11881 gcatcacgct gagctatttt atcggcaacg ctggcgggaa taatggcgta ttcaccttcg
    11941 ctattgttat cgaccaacaa gcgagcaatc gccagacgac cattaatcaa ctgcgcctgt
    12001 gtgagcttat cgacaaaaat cttcttaatc agattgccgt cagtgaagtt aaaaccaata
    12061 tcgccattgg caatggtgat tcggttcatt tcaatgagct gcttcacctg agctttatat
    12121 tctttcgcca acgcagcttg tttttgttgc tcgctaagct gtttatcacg ctcaagctgt
    12181 gcctttttat tttcttctac cgccgcccga gcttcacgcg cctgaacgcg cgattttttc
    12241 gccgttctct ccaccttcgc cgcttttttg ctggtgacta atcctgcttt gagcaactgc
    12301 tcttgtaagg taagttttgc catctttgtt tccaaacccg ctgaataatt tttagcgagt
    12361 atacctgtaa acgccgtggg tgtattgggg gcaagtccct ttcgcccctg ctaaataagg
    12421 cgcgtcaggc atcggcacac ggctgcattc ttgatctccg actcatcaaa ataaacaccg
    12481 ccaacccagc aacaagaata cccggtgcag aagcggccat tacgccgacc gtgccagtgc
    12541 ctaatgccag cattttcccg gccagtaacg gaccgctcat agcccccaga cgccctaccg
    12601 ccacggctgt tcccacacct gttgcgcgga tctgcgaact gtaaaacaac ggtgccaggg
    12661 catacaaaac gctttgccca cctgtcgcaa acaaccccgc gacaaatccc gccagcaaca
    12721 taccgttaaa tgacgacacc gttccaagcg ccagcagcga agctaacatg ccgctataaa
    12781 tcagtagcga catggttact ggacgcagct tatccatcaa tgcgcccaac attaacgtcc
    12841 cgcttgccgc ccccatttgc agggcaaaca tcacccctgc cgcctgcgat ggctggaatc
    12901 cttgctccac caaaagtagc ggtagccagt tgatcaacat ataaaccacc agcagagtga
    12961 agaaataaca caaccacagc agcagcgtcg cggttgccgt ttctggcgca aataaggcac
    13021 gcagtggtgg cgcagcctgt ttttcgccag cgaaaaccgc tgactccggc agccagcgca
    13081 ttaatagcgg caccagaatc aacggcacca caccacctac ccaaaacacc gtttgccatg
    13141 ctaagtttgc ccccgcgaaa cccagtgtcg ccgccagcgc cgcgccaatg ggaacaccgc
    13201 aatacatcag gctcactgcc gtcccacgaa aacgtggacc cgcggcttca gacgtcagag
    13261 cgataagatt cggcaacgcc gcccccagcc cgacaccggt catcagccgc gcaaagacca
    13321 gtgaggggaa atcccaggca atcgccgttg ccagtgagaa caaaccaaac agcgcaactg
    13381 agccaatcaa aatacgctta cgaccataac ggtctgccag catcccgcca accaacgcgc
    13441 cgggtagcaa accgagtatt ccggcgctaa atatccagcc catttgcatt ttatcgagtg
    13501 cgaaagcctg ggcgatgcca cccgccgcaa tgccagccgc ctgaagatcc agcccttcca
    13561 tcagagcgac caaaaaacaa agcccgatgg tcagcatcag gcgggatgaa gatgatgaag
    13621 gggtacgagt cgacataaga tgaacctcat tgcgagtgca ggtgaatcgc gactgataca
    13681 ttgccagccg cgtaatcaga actatttaat gactgcctac gggcgaaatg taggccggat
    13741 aaggcgttca cgccgcatcc ggctgtcgtg ctgggatggg catacaaaat gcacgttatc
    13801 ggcagtcatc attatttgtt gttgcgcaga tccagcgcca cgtcgacgat catatcctcc
    13861 tggccgccaa ccatccggcg tttgcccagc tcaacgagaa tatccaccgc acttaagcca
    13921 taacgcgccg ccgccgtttc acagtgacgc aggaagctcg agtaaacacc agcgtatccc
    13981 agcgccagcg tttcgcgatc gactcgtacc ggtcgatcct gcaacggacg caccaggtcg
    14041 tcggcggcat ccattaacgc atagagatcg gtcccatgct gccagcccag tttatccgcg
    14101 gcggcaataa acacttccag cggtgcgtta cctgcgcccg cgcccattcc cgcgaggctg
    14161 gcgtcgattc ggtcgcagcc ctcttccacc gccgcgatag agttcgccac gccaagactc
    14221 aggttatggt gagcgtgcat gccagtttgc gtttctggtt tcagctctgc tttcagggcg
    14281 cggaaacggt cacggatatc gctcatgttc atcgcaccgc cagaatccac cacataaata
    14341 caggtcgcac cgtagccttc catcagcttt gcctgcttgg cgagattctc cggcgtggtc
    14401 atatggctca tcatcagaaa accaacggtg tccattccga gctcgcgggc atactgaata
    14461 tgctgggcgg aaacatcagc ttcggtacag tgcgttgcca cacgaaccac ccgcgcgcca
    14521 gcctgccagg catttttcag atcgtgaata gtgccgattc ctggcagcaa caacgtcgcg
    14581 attttggcgt gcttcaccat atccgccgct gcttcaatcc attcaaggtc gctatgtgcg
    14641 ccgaaaccat agttaaagct ggaaccctgc aaaccgtcgc catgggccac ttcaatcgaa
    14701 tccacgcggg catcgtccag tgctttggca atctggcgaa cgttttccag cgaatactga
    14761 tgacgaatgg cgtgcatacc gtcacgcaac gtgacgtccg agatataaag ttttttaccg
    14821 ttcatgccgc ttctcctgcc ttgcgcgcca gtgactgggc cattttttcc gctgtcgcca
    14881 gcgcactgga agtcataatg tcgaggttgc ccgcataggc aggcagataa tgcgctgcgc
    14941 cttcgacttc ca
//
LOCUS       DAMZET010000103        14686 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.103, whole genome shotgun sequence.
ACCESSION   DAMZET010000103 DAMZET010000000
VERSION     DAMZET010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.103"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            83..1897
                     /locus_tag="POB34_003163"
     CDS             83..1897
                     /locus_tag="POB34_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI5920057.1"
                     /translation="MIVRILLLFIALFTFSVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPIYSLGIDTWWYDVNKAAKLPSASKQGE"
     gene            1898..2992
                     /locus_tag="POB34_003164"
     CDS             1898..2992
                     /locus_tag="POB34_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HDI5920058.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            2992..4017
                     /locus_tag="POB34_003165"
     CDS             2992..4017
                     /locus_tag="POB34_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5920059.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            4019..5608
                     /gene="yejF"
                     /locus_tag="POB34_003166"
     CDS             4019..5608
                     /gene="yejF"
                     /locus_tag="POB34_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HDI5920060.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(5612..5956)
                     /locus_tag="POB34_003167"
     CDS             complement(5612..5956)
                     /locus_tag="POB34_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920061.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(6289..7479)
                     /gene="bcr"
                     /locus_tag="POB34_003168"
     CDS             complement(6289..7479)
                     /gene="bcr"
                     /locus_tag="POB34_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HDI5920062.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(7507..8202)
                     /gene="rsuA"
                     /locus_tag="POB34_003169"
     CDS             complement(7507..8202)
                     /gene="rsuA"
                     /locus_tag="POB34_003169"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HDI5920063.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            8352..10112
                     /locus_tag="POB34_003170"
     CDS             8352..10112
                     /locus_tag="POB34_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDI5920064.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            10237..10521
                     /gene="rplY"
                     /locus_tag="POB34_003171"
     CDS             10237..10521
                     /gene="rplY"
                     /locus_tag="POB34_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HDI5920065.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(10660..11667)
                     /gene="yejK"
                     /locus_tag="POB34_003172"
     CDS             complement(10660..11667)
                     /gene="yejK"
                     /locus_tag="POB34_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HDI5920066.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     LESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAQAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            11849..12076
                     /locus_tag="POB34_003173"
     CDS             11849..12076
                     /locus_tag="POB34_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HDI5920067.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            12096..13856
                     /gene="pbgA"
                     /locus_tag="POB34_003174"
     CDS             12096..13856
                     /gene="pbgA"
                     /locus_tag="POB34_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HDI5920068.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            13931..14007
                     /locus_tag="POB34_003175"
     tRNA            13931..14007
                     /locus_tag="POB34_003175"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13965..13967,aa:Pro,seq:ggg)
     gene            14249..14466
                     /locus_tag="POB34_003176"
                     /pseudo
     CDS             14249..14466
                     /locus_tag="POB34_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
BASE COUNT         3621 a         3796 c         3672 g         3597 t
ORIGIN      
        1 aggtgtgctt acgtccctta ttattcatag tgaaagcatg ccggattgcg gctaatgatg
       61 agtaaaagga aatccgttgc agatgattgt gcgcatactg ctgctgttta tcgctctgtt
      121 cacctttagt gtgcaggcgc aggctatcaa ggaaagctat gcctttgccg tgctgggcga
      181 accccggtac gcgtttaatt tcaaccattt tgattatgtg aaccccgccg cgccaaaagg
      241 tgggcagata acgttgtcag ccctcggcac cttcgataat ttcaaccgct atgcactgcg
      301 cggcaacccg ggcgcacgca ccgagcagct gtacgacacg ctatttacga cttccgatga
      361 cgaaccaggc agttattacc cgctgattgc tgaaagcgca cgctatgctg acgattattc
      421 ctgggtggag gtcgctatta atccgcgcgc ccgttttcat gatggttcgc ccattactgc
      481 ccgcgatgta gagtttactt ttcaaaaatt tatgaccgaa ggcgtgccgc aatttcgtct
      541 ggtctacaaa ggcaccaccg tcaaagccat tgcgccgtta accgtgcgca ttgagttagc
      601 taaacccggc aaagaagata tgttgagtct gttttcgctg ccggtatttc cagaaaagta
      661 ctggaaggat cacaaactta gcgacccgct cgccacgcct ccgcttgcca gtggtccgta
      721 ccgcattacg tcctggaaaa tggggcaaaa tattgtctat tcccgcgtga aagattactg
      781 ggcagcaaac ttaccggtaa accgcggacg ctggaatttc gacaccattc gctacgatta
      841 ttacctcgat gataatgtcg cctttgaagc gtttaaagca ggtgcctttg atttgcgtat
      901 ggaaaacgac gccaaaaact gggccacgcg ttataccggt aaaaatttcg ataaaaaata
      961 catcatcaaa gatgagcaaa agaacgaatc agcccaggat acgcgctggc tggcgtttaa
     1021 tatccaacgt ccagtattca gcgatcgccg ggtgcgggaa gcaatcactc tcgcctttga
     1081 ctttgaatgg atgaacaagg cgttgtttta caatgcctgg agtcgcacga acagttattt
     1141 tcagaatacc gaatacgcgg ccagaaatta ccccgacgcc gcggagctgg tgcttctggc
     1201 accaatgaaa aaagatctac cgccagaagt cttcacacaa atctaccagc cgccggtatc
     1261 caaaggcgat ggctacgatc gtgacaacct gttaaaagcc gacaaacttc tcaacgaagc
     1321 gggctgggtg ctgaagggtc agcaacgcgt taatgccaca acgggtcagc cactcagctt
     1381 tgaattattg cttcccgcaa gcagcaatag tcagtgggta ttgccgttcc agcacagcct
     1441 gcaacggctg ggtatcaaca tggacattcg caaggtggat aactcgcaaa tcactaaccg
     1501 catgcgcagt cgcgactatg acatgatgcc gcgcgtatgg cgggcgatgc cgtggcccag
     1561 ttccgattta cagatttcct ggtcatcgga atatatcaat tccacttata atgcccccgg
     1621 cgtgcaaagc ccggttatcg actcgctgat caaccaaatt attgccgcgc agggaaataa
     1681 agaaaaatta ctgccgttgg ggcgagcact ggatcgcgta ttaacgtgga attattacat
     1741 gctgccaatg tggtacatgg cggaagaccg tctcgcctgg tgggataaat tctcccagcc
     1801 ggccgtgcgc cccatctata gcctcggtat cgatacctgg tggtatgacg tcaataaagc
     1861 ggccaaactg ccgtccgcca gcaaacaggg agagtagatg ggcgcttacc tgattcgccg
     1921 tctgttgctg gtgatcccaa cattatgggc gattatcacc atcaactttt tcatcgtgca
     1981 aattgcgcct ggcggtccgg tcgaccaggc catcgccgcc attgagtttg gtaatgccgg
     2041 agtattaccc ggcgcaggcg gtgaaggtgt tcgtgccagc catgcgcaaa cgggtgtcgg
     2101 caatatcagc gacagtaatt accgtggcgg acgcggatta gatccagaag tgatcgctga
     2161 gatcactcat cgctacggtt ttgataagcc gatccacgaa cgttacttca aaatgctctg
     2221 ggactacatc cgctttgatt ttggcgatag cctgtttcgc agcgcctcgg tgctgacgct
     2281 gattaaagac agtctgccgg tttccatcac cctcggattg tggagcacgc tgattatcta
     2341 tctggtgtcg attccgttag gcattcgcaa agctgtttat aatgggagcc gctttgacgt
     2401 ctggagtagc gcatttatca tcatcggcta cgccattccg gcctttttgt ttgccatcct
     2461 gctgattgtc ttcttcgcgg gcggcagcta tttcgacctg ttccctctac gcggcctggt
     2521 ttccgctaac tttgattcgc tgccgtggta tcagaaaatc accgattatc tgtggcatat
     2581 cacgctgccg gtgctggcga cagtgattgg tggctttgcg gcgctgacca tgctgacaaa
     2641 aaactcattc cttgatgaag tgcgcaagca atacgtggtg accgcgcgtg cgaaaggggt
     2701 aagtgaaaaa aatattctct ggaaacatgt gttccgcaac gccatgctgc tggtgattgc
     2761 cggttttccg gcgacgttta tcagcatgtt ttttaccggc tcgctgctga ttgaggtgat
     2821 gttttcactc aatggtctgg gcttactggg ctacgaagcg accgtctcgc gcgattatcc
     2881 tgtaatgttt ggtaccttgt atattttcac cctgattggc ctgctgctga atattgtcag
     2941 tgatatcagc tatacgctgg ttgatccgcg tattgatttt gagggacgtt aatgtcgcga
     3001 ctcagccccg tcaatcaggc ccgatgggcg cgttttcgtc ataaccgtcg cggctactgg
     3061 tcgttatgga ttttcctcgt cttgtttggt ttgagtttgt gttctgaact tatcgccaac
     3121 gataaaccgt tgctggtgcg ttatgacggc agttggtatt tcccgttatt gaaaaactac
     3181 agcgaaagcg attttggcgg cccgctggca agtcaggctg attatcagga cccgtggctg
     3241 aaacaacggc tggaaaataa cggctgggta ctgtgggcac cgattcgctt tggtgctacc
     3301 agtatcaact ttgctaccga taagcccttc ccttctccac cctcccggca aaactggctg
     3361 ggaacggatg ccaacggcgg cgatgtgctg gcacgcattc tctatggcac gcggatctcg
     3421 gttctgtttg gcctgatgct gactctctgt tccagcgtga tgggcgtgct ggcgggggcg
     3481 ctacaaggct attacggcgg taaagtcgat ctctggggac aacgctttat tgaagtatgg
     3541 tcggggatgc cgacgctgtt tttgattatt ttactttcca gcgtcgtaca gcctaacttc
     3601 tggtggctgc tggcaattac tgtcttgttt ggctggatga gtctggtcgg cgtggtgcgg
     3661 gcggagtttt tacgtactcg taatttcgac tacattcgtg cggcacaggc gcttggcgtc
     3721 agcgatcgca gtatcatcct gcgtcatatg ttgcctaatg ccatggtcgc gaccctcacc
     3781 tttttaccgt ttattttatg tagttcgata accaccctga cctcgctcga tttcctcggc
     3841 ttcggtctac cgctcggttc accgtcactc ggtgaactgc tgttacaagg gaaaaataac
     3901 cttcaggccc cgtggcttgg gatcaccgcc ttcttgtcgg tggcgatatt attgtctttg
     3961 ctgatcttta ttggtgaagc cgtccgcgac gcatttgatc ctaataaggc ggtgtagcat
     4021 gacgcaaact ctgttagcga ttgaaaattt gtcggtgggt tttcgccatc agcaaaccgt
     4081 acgtacagta gtcaatgatg tttcactaca gattgaggct ggcgaaacgc tggcgctggt
     4141 gggtgagtca ggttcaggca aaagcgttac cgcgctgtca attttacgcc tgctcccttc
     4201 cccgccggtt gaatatctct ccggcgatat tcgttttcat ggcgaatcgc tgcttcatgc
     4261 cagcgatcaa acgttgcgcg gtgtacgcgg taataagatc gccatgattt ttcaggaacc
     4321 gatggtgtcg ttaaatccat tgcataccct ggaaaaacag ctttatgaag tgctttcact
     4381 ccaccgcggg atgcgtcggg aagcggctcg tggcgaaatt cttaactgcc ttgatcgcgt
     4441 tggtatccgc caggcggcaa aacggctgac agattatccg catcagctct ccggcggcga
     4501 acgccagcgg gtgatgattg cgatggcgct gttaacgcga ccggaattat taattgccga
     4561 tgaaccgacc accgcactgg acgtctctgt ccaggcgcag attttacagc tgttgcgcga
     4621 actgcaaggc gagctgaata tgggcatgct gtttattact cataacctca gcattgtcag
     4681 aaaactggcc caccgcgtgg cggtaatgca aaacggtcgc tgtgtcgagc aaaataacgc
     4741 cgctacgcta tttgcctcac ccactcatcc ttacacacaa aagctactca acagtgaacc
     4801 gtcaggcgac ccggtgccgt tgccagaacc tgcctcaacg ttgctggatg ttgaacagct
     4861 tcaggttgcc ttccccattc gcaaagggat tttgaagcgc attgtggatc ataatgtggt
     4921 ggtgaaaaac atcagtttta cgctacgagc gggtgaaaca ctgggtttag tgggcgagtc
     4981 cggttccggg aaaagtacga cgggactggc gctgctgcga ctgattaatt ctcagggcag
     5041 catcatcttt gacggtcagc cattgcaaaa tttaaatcgc cgccagctgt tacctattcg
     5101 tcatcgcatt caggtggtat ttcaggatcc aaactcctcg ctcaacccac gactcaacgt
     5161 tttgcagatt attgaggaag gcttacgggt tcaccagccg acgctttctg ccgcacaacg
     5221 cgaacaacaa gtgatagccg tgatgcatga agtgggatta gatcctgaaa cacgccaccg
     5281 ttatccggcg gagttctctg gtggtcagcg acaacgtatt gcgattgcca gggcattaat
     5341 tcttaagccc tcgctgatca tacttgatga accgacatca tcactcgaca aaacggtaca
     5401 ggcgcaaata ttgacgctat tgaaatcatt gcaacaaaag catcaactgg cctatttgtt
     5461 tatcagccac gatttgcacg ttgtccgcgc gttatgtcat caggttatcg tactgcgaca
     5521 aggggaagta gtggaacaag gaccgtgcgc gcgcgtgttt gccgcaccgc agcaggagta
     5581 tacgcgtcag ctactggcgt tgagctgacg cttaaaaagg attgtagttt gaaaagggtt
     5641 cggcaattgc cacaccaaaa tttttcaatc ggcatgttgc agcaaactcg tcctggcgat
     5701 ttacaaacag gcacggctca ccttcacact ccagcaccga acatggaact tcgatgtcgc
     5761 ttaacgcctg gcttagtttg tacatcaccg accatgcatt atcaccatcc gggctaagca
     5821 atttaagcgc caccaggctg ttgtcaccgc acggtccatt ttgcggaatc ggttcaacct
     5881 tcgaacctgc gaaaccagca gaccagcgaa agttctgcgg tagacgatga acgattgaga
     5941 gttgtaatga agtcacttat tttccccgga agtacattta cttcacaatt tgtttacatc
     6001 aattttaaca catcatcaac aatccgtctt tatacagatc gtaaagattg atgcctcgcc
     6061 tgcgtggcct ctatggctct gatttaagta atatctgcgg gcgtggtcac gtttgcgggg
     6121 ctatatgtac ccgtttctgc gatctaactc aaccttttta actacaatga tgtgactttt
     6181 tacataaatt gattttacat aaaataaaca tatatcgggg gaatgataga tttgtggttg
     6241 acatgcatca acaaaggaag ccttttagct tcctcgttgt gcaatagatc accgtttttt
     6301 cggtcgactg gcgtacagac agaagagaat ggagctggtt gcgcagaatg caattgacca
     6361 aatcatcggc catgcagagt taaaggtcgc aagagaaagc aatgcgccaa caattgcccc
     6421 tatgccaaaa cggaaggttc ctgccagcga agatgccgtt cccgccatat ggggaaactc
     6481 atcaagaatg accgccatcg cattggatga caccatcgac acgcagccca caaacgccgc
     6541 aacgccaacc accagcgacc agaatcccag ccccagcagc gcactgatga ccatccacgc
     6601 cgccataata aactgtatcc acaaccccga gcggaacata tttaacgcgc caatacggcg
     6661 gacgaagcgg ctgttaaaaa tggtcatcac gaacaggaaa acaatgttta gtgcaaagta
     6721 ataaccaaag ttttccggcg cgacgtggtt aatttcaatg tagacaaacg gtccggcgct
     6781 caggaacgag aacattccgg caaagctgaa accgctggcc agcatataac tcaggacacg
     6841 cttatgacgg aacagcgccg caaagttacc aatagtggta cgaatgtgaa aaggctggcg
     6901 acgctccggt ggtaaggttt ctttgatcag aaagaagatc attgccgaag ccagaatcgc
     6961 cgccacggcg aggatccaga agatgtaatg ccagctcagc cacaccagca cccagccgcc
     7021 aactatcggt gccatcagcg gtgcaatggt tgtcaccagc atgacaaacg acatcatccg
     7081 cgagaactct tctttcgggt agatatcacg catcaaagcg ttgataacca cgctggccgc
     7141 agccgccgcc agcccgtgga agaaacgcat cacaatcagc tgatcgatgg tttgtgccaa
     7201 cgcacacgcc accgcggcgg cggcaaacac cagcgtaccg ccaagaacca ccggcttacg
     7261 cccgaagctg tctgccatcg gcccgtagat taactgcccc aacgcaaagc ccagaatata
     7321 agtactgagg gtcatctgcg tactgcccgc cggtacgcca aactgcgctg aaattaccgg
     7381 tagcgcgggc agatacatat caatcgacag cggcatcaac atggccagca ggccaaggat
     7441 aaaaacaata gcaaacgacg aatgctgtcg ggtggtcaca acgggctcct gaaagtcatt
     7501 gaaaagttag acgacgctgg caatttcttc ttcagttaac ggacgatatt caccgggggc
     7561 taaatcagca tccagcgtaa tgccgccaat acgttcacga tgcagctcaa ccacatggtt
     7621 acccacggcg gcgaacatgc gtttcacctg atgataacgc ccttcgctga tggtcagacg
     7681 aacctgcgtt ggggtaatca cttccagcac cgccggctta gtgagatctt tttcgttatg
     7741 cagctgcacg cctttagcaa attgctctgc cgtatcgtca gctacgggtg attccagcgt
     7801 caccagatag gtcttctcgc aatgatggcg cggagaagta atgcggtgcg accactgacc
     7861 atcatctgtc atcagcacca gaccggtggt atcgatatcc agacgccctg cggcgtgcag
     7921 tttccaggct accggttcat caagaaaata gagcaccgtt gggtgatcag ggtcgtccgt
     7981 ggagcaaacg tagccctgag gcttattgag catgaagtaa cgtggaccgt gttgctgcgc
     8041 cagcgggttg ccatcgtaag cgacatcatg ttcaggaagc agtttgaacg ctgcattacg
     8101 gacgatttcg ccatcgacgg tgacacgatt gccgcggatt tcacgcccgg caatagcacg
     8161 gctaacgccg agttgctgtg cgataaattt atcaagtcgc atgtgtgtga ttttgcctgt
     8221 aaaaaacgga ggtcgggtaa tgacccgaaa atctgaactg ttgtctgccc agtatagcgg
     8281 tctaataaca tccctcaagg gaaaaagatc catggcatac tattagcaga ataatctacc
     8341 tacgcgagac catgattttt acacttcgcc catatcagca agaagccgtg gatgccacgc
     8401 tcaaccattt tcgtcgtcat aaaacccctg ccgttatcgt gctgcctact ggtgcaggta
     8461 aaagcctggt gatagcggaa ctggcgcggc tggcgcgtgg tcgcgtgctg gtgctggcac
     8521 acgttaaaga actggtggcg caaaaccatg caaaatatca ggcactggga ctggaagccg
     8581 atatttttgc cgccgggcta aagcgcaaag agagccacgg taaagtggta tttggcagcg
     8641 tgcagtcggt cgcccgtaat cttgatgcct ttcagggtga attttcgctg ttgattgtcg
     8701 atgaatgtca ccgtattggt gatgatgaag agagccagta tcagcaaatc ctcactcacc
     8761 tgacaaaagt gaatccccac ttacgcctgc tggggctgac tgccacgcct tttcgattgg
     8821 gcaaaggctg gatctaccag ttccattatc acggcatggt acgcggcgat gagaaagccc
     8881 ttttccgtga ctgcatttat gagctgccgc tgcgttatat gattaaacac ggctatctga
     8941 cgccgccaga acgactggat atgccagtag tacaatacga tttcagccgc ttgcaggcac
     9001 agagtaacgg gctgttcagc gaagctgatc tcaaccgtga gctgaaaaaa cagcaacgca
     9061 ttacgccaca tatcatcagc cagatcatgg agttcgccga aaagcgcaaa ggggtaatga
     9121 ttttcgccgc gacggtcgaa cacgcaaaag agattgtggg attactgccc gccgaagatg
     9181 ctgcactgat tactggcgac acccccggcg ctgagcgcga tgtgttaatt gaagatttta
     9241 aagcccagcg ttttcgctat ctggtcaacg tcgcagtact gaccaccgga tttgacgccc
     9301 cgcacgtcga tcttatcgcc attctgcgcc ctaccgaatc agtgagtctt taccaacaaa
     9361 ttgtcgggcg cggtctgcgt ctcgctcctg gcaagactga ttgcttaatt cttgattatg
     9421 cgggtaatcc tcacgatctc tacgcgccgg aagttggtac accgaaaggc aaaagtgaca
     9481 acgtcccggt acaggttttc tgccctgcct gcggttttgc caacaccttt tgggggaaaa
     9541 cgaccgccga cgggacattg attgaacact ttggtcgtcg ctgtcaggga tggtttgaag
     9601 atgacgacgg tcatcgcgaa cagtgtgact tccgtttccg ttttaaaaat tgcccgcaat
     9661 gtaacgcaga aaatgatatt gccgcccgcc gctgccgcga atgtgacacc gtactggttg
     9721 atccggacga tatgttaaaa gcggcgctac gactgaaaga cgcgctggta ttacgctgta
     9781 gcggcatgtc tttgcaacat gggcacgacg agaaaggcga atggttgaaa atcacctatt
     9841 acgatgaaga cggcgcggat gtgagtgagc gtttccgtct gcaaacgccc gcccagcgaa
     9901 ctgccttcga gcagcttttt atccgcccgc atacgcgcac accgggcatc cctctgcgct
     9961 ggatcaccgc cgccgatatc ctcgcccagc aagcgttact gcgacacccg gattttgtcg
    10021 tcgcccgcat gaaaggtcag tactggcaag tgcgtgaaaa agtgttcgat tacgaaggtc
    10081 gttttcgtcg ggcgcacgaa ttacgcggtt aatggtagtt ttgattgatg tataaggcga
    10141 ttgagtatag aatctcgccc gcttttgcat acgcaaagca gatcacttac ctgttgctgg
    10201 gtcgcctgta gcaggattaa tttaagagag aaagaaatgt ttactatcaa cgcagaagta
    10261 cgtaaagagc agggtaaggg tgcgagccgc cgcctgcgtg ccgctaacaa gttcccggca
    10321 atcatctacg gtggcaaaga agcgccgctg gctatcgagc tggatcacga caaagtcatg
    10381 aacatgcaag ctaaagctga attctacagc gaagttctga ccatcgttgt tgacggtaaa
    10441 gaaatcaaag ttaaagctca ggacgtacag cgtcacccgt acaaaccgaa gctgcagcac
    10501 atcgacttcg ttcgcgctta attgctgaat aagttgtaaa aaaccccgct ccggcggggt
    10561 tttttgtatc tgcagattat gcctgattac ggtattgcta ttttttgcag gccagataag
    10621 gcgttcacgc cgcatctggc attcagaaca caaaaccgat taattgccgc cagacgtccg
    10681 gcgctgtaat tggtcgcgca aattcggcgg cgtgccttta atggtcaagg tatcggttgc
    10741 tggatcccag aagatgcgtt cacctaacag catcgcatca aagttaatag tcaagccacc
    10801 gccgctaccg gcaaacttcg tcaactgacg cagtgtgcta cggtctgccg ggaagctctc
    10861 ttccagttcg tagccttttt cagccgcaaa ctccgtgaaa ctcacttcgc tgacgcctgc
    10921 cagctctttc gacagcgact ccagttcaat ctcttcgcct gcttgcagct gctcgttaca
    10981 gtagctataa acctgctggc gcacattctg ccgctcggct ttatccagtt gcgcttgtgc
    11041 agtgaaatca tccaccgctt gcagcaagcc acggttttgc gcctttgcat tcagcccttc
    11101 gctggcacca aggaaatcca taaagaaatc ggcgaccttg cgccctaccc gccctttcag
    11161 gaaagttaaa tagcgggtcg attccaggtt ggtttcccat tcggttaaat cgatgcgcgc
    11221 gacgatatcc gcatggttga tatcgagata gtgcgttggg ttgatgtcga gattttcgtt
    11281 aacgcgcata ctgctcaggt tgctcaggac tgccaccagc agatactcca ccgccagata
    11341 acggtagtgg caaaacagca cgaatccacc gtcagcaaaa ggatatttcg ctaattcgtc
    11401 acgcaggcga ccagttgccg cgcggctaaa tgccagaaag tcctcttcgc cctgacgctg
    11461 taagcgcagc gtctgcgcca gttcactctc ttcgctaaac aagccgtaag ctttattttt
    11521 ggcgctatag acccgatgca gttctgccac catctcaacg acggtttctg tcggttccag
    11581 caatgaatcg cgcaacacca gctcaaggtt ctgctcatca cgcttgataa gctggtgcag
    11641 agcaatctgg ttgatatcca gactcatgat aaactctcct ttaagaccgg gcggtattca
    11701 accaccgccg gttaagcgac gcaataaaag atggcggtca ttttacagaa ggcagcgtaa
    11761 cgagaatgat ttaacgcata aaaaagcaga aaaaaaaccg ttgctacggt aatatgttgc
    11821 cctttcatca actaactgat ttcgatttat gccacaaatt tcccgctaca gtgatgaaca
    11881 ggttgaacaa ttgctcgcag agctgctcaa cgtactggaa aaacataagg ctccgaccga
    11941 cctttccctg atggtgttag gcaacatggt cactaacctt atcaacacca gcattgcccc
    12001 ggcccaacgc caggcaattg ccaactcttt tgcccgcgcc ttacagtcct ctatcaacga
    12061 agacaaagcg cactaaggga aacagataac aggttatggt aactcatcgt cagcgctacc
    12121 gtgaaaaagt ctcccagatg gtcagttggg ggcactggtt tgcactgttc aatattctgc
    12181 tttcgctcgt cattggcagc cgttacctgt ttatcgccga ctggccgacc acgcttgctg
    12241 gtcgcattta ttcctacgta agcattatcg gccatttcag cttcctggtg ttcgccacct
    12301 acttgctgat cctcttcccg ctgaccttta tcgtcggctc ccagaggctg atgaggtttt
    12361 tgtccgtcat tctggcaacg gcgggaatga cgctattact gatcgatagc gaagtcttta
    12421 ctcgtttcca tctccatctt aatcccatcg tctggcagtt ggttatcaac ccagacgaaa
    12481 atgagatggc gcgcgactgg cagctgatgt tcatcagcgt gccggttatt ttattgcttg
    12541 aactggtgtt tgcgacgtgg agctggcaaa agctgcgcag cctgacgcgt cgtcgacgct
    12601 tcgcgcgccc gctggccgca ttcttattta tcgcctttat cgcctcgcat gtggtgtata
    12661 tctgggccga tgccaacttc tatcgcccga tcaccatgca gcgcgctaac ctgccgcttt
    12721 cgtacccgat gacggcgcga cgttttcttg agaagcatgg cctgcttgat gcgcaggagt
    12781 atcaacgccg tcttattgag caaggtaatc cagacgccgt ttccgttcag tatccgttaa
    12841 gcgaactgcg ctatcgcgat atgggcaccg ggcagaatgt cttgttgatt actgtcgatg
    12901 gcctgaacta ctcacgcttc gagaagcaga tgcctgcgct ggcaggtttt gctgagcaaa
    12961 atatttcgtt cacgcgccat atgagctccg gcaacactac agacaacggc atctttggcc
    13021 tgttctatgg catctcgccg agctatatgg acggcattct gtcgacccgt acgcctgcgg
    13081 cattaattac tgcgcttaat cagcaaggct atcagctggg gttattctca tcagatggct
    13141 ttaccagccc gctgtatcgc caggcattgt tgtcagattt ctcgatgccg agcgtacgca
    13201 cccaatccga cgagcagacc gccacgcagt ggatcaactg gctgggacgc tacgcacaag
    13261 aagataaccg ctggttctcg tgggtttctt tcaatggtac taacattgac gacagcaatc
    13321 agcaggcatt tgcacggaaa tatagccggg cggcaggcaa tgtcgatgac cagatcaacc
    13381 gcgtgctcaa tgcactgcgt gattctggca aactggacaa tacggtggtg attatcactg
    13441 ccggtcgggg tattccactg agcgaagagg aagaaacctt tgactggtcc cacggtcatc
    13501 tgcaggtgcc attagtgatt cactggccag gcacgccggc gcagcgtatt aatgctctga
    13561 ctgatcatac cgatctgatg acgacgctga tgcaacgcct gctacatgtc agcacacctg
    13621 ccagcgaata ttcgcaaggt caggatttgt tcaaccctca acgccgtcat tactgggtta
    13681 ccgcagcgga taacgatacg ctggcaatta ccaccccgaa aaagacgctg gtgctgaaca
    13741 ataacggtaa ataccgcact tacaacttac gtggtgaaag agtgaaagat gaaaaaccac
    13801 agttaagttt gttattgcaa gtgctgacag acgagaagcg ttttatcgct aactgattaa
    13861 ttataaatca gttagcgaaa tatcttactt gcaatcggtg tggaaaacgg tagtattagc
    13921 agccacgagt cggcacgtag cgcagcctgg tagcgcaccg tcatggggtg tcgggggtcg
    13981 gaggttcaaa tcctctcgtg ccgaccaaaa atcccttaaa aaccagccta ttatggctgg
    14041 tttttttatg attgtttttt atgcggagaa ataccgggga ataattgggg taaaacacct
    14101 ttaggctatt cacttagcct accaccatca aaagctttcc gaatgtatcc atctcttttt
    14161 tcttatgcag ctaaaacgaa gtcttgatac tgtttattta cacagataag aataatactg
    14221 tctacaaacg cagtacaaaa gaagtgttat gcgtattgag atctccatag ccaaagacaa
    14281 aatgaccaaa tgccaaacgg cgctgtggac gcgttaaagg aagaattaac ccaacacatt
    14341 ggcaagcgtt atgacgatgt ggagatgatc gtaaaagcca ccagcaacga tagtttttca
    14401 gttagacata ctgcagataa agattgagct aaaactttcg ttcaggaaac tctgaatgat
    14461 acctgagagt ctactgaaga gttgtttgtt cactagttag cacgtaggaa atcattcaat
    14521 actcgcacta tcggaaattc accagccagt cgtagcacgt ccttgcaaac gacgtgccta
    14581 cggtttcaat cacctctcac catcagttct atcaataata attaaacagc gcctagacaa
    14641 aataaataac aacaagtcaa caacctcgac aggatgccga tgggat
//
LOCUS       DAMZET010000104        14633 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.104, whole genome shotgun sequence.
ACCESSION   DAMZET010000104 DAMZET010000000
VERSION     DAMZET010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.104"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..249)
                     /locus_tag="POB34_003177"
     CDS             complement(<1..249)
                     /locus_tag="POB34_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HDI5920069.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVF"
     gene            complement(359..1282)
                     /locus_tag="POB34_003178"
     CDS             complement(359..1282)
                     /locus_tag="POB34_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDI5920070.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(1284..1931)
                     /locus_tag="POB34_003179"
     CDS             complement(1284..1931)
                     /locus_tag="POB34_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HDI5920071.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPSVTDMSSAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(1942..2526)
                     /gene="rimJ"
                     /locus_tag="POB34_003180"
     CDS             complement(1942..2526)
                     /gene="rimJ"
                     /locus_tag="POB34_003180"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HDI5920072.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            2762..3970
                     /gene="mdtH"
                     /locus_tag="POB34_003181"
     CDS             2762..3970
                     /gene="mdtH"
                     /locus_tag="POB34_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HDI5920073.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            4034..4681
                     /gene="grxB"
                     /locus_tag="POB34_003182"
     CDS             4034..4681
                     /gene="grxB"
                     /locus_tag="POB34_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HDI5920074.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            4815..5375
                     /locus_tag="POB34_003183"
     CDS             4815..5375
                     /locus_tag="POB34_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDI5920075.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            5481..6527
                     /gene="pyrC"
                     /locus_tag="POB34_003184"
     CDS             5481..6527
                     /gene="pyrC"
                     /locus_tag="POB34_003184"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDI5920076.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            6601..6846
                     /gene="dinI"
                     /locus_tag="POB34_003185"
     CDS             6601..6846
                     /gene="dinI"
                     /locus_tag="POB34_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HDI5920077.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            7136..7390
                     /gene="bssS"
                     /locus_tag="POB34_003186"
     CDS             7136..7390
                     /gene="bssS"
                     /locus_tag="POB34_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HDI5920078.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            7505..8623
                     /gene="solA"
                     /locus_tag="POB34_003187"
     CDS             7505..8623
                     /gene="solA"
                     /locus_tag="POB34_003187"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HDI5920079.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIHHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            8671..8784
                     /locus_tag="POB34_003188"
     CDS             8671..8784
                     /locus_tag="POB34_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HDI5920080.1"
                     /translation="MCRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            9045..9611
                     /locus_tag="POB34_003189"
     CDS             9045..9611
                     /locus_tag="POB34_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HDI5920081.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISSADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            9615..10190
                     /locus_tag="POB34_003190"
     CDS             9615..10190
                     /locus_tag="POB34_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HDI5920082.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(10232..11284)
                     /locus_tag="POB34_003191"
     CDS             complement(10232..11284)
                     /locus_tag="POB34_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDI5920083.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            11509..12429
                     /gene="lpxL"
                     /locus_tag="POB34_003192"
     CDS             11509..12429
                     /gene="lpxL"
                     /locus_tag="POB34_003192"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HDI5920084.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            12601..13827
                     /gene="mdtG"
                     /locus_tag="POB34_003193"
     CDS             12601..13827
                     /gene="mdtG"
                     /locus_tag="POB34_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HDI5920085.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            13910..14284
                     /locus_tag="POB34_003194"
     CDS             13910..14284
                     /locus_tag="POB34_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HDI5920086.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(14285..14512)
                     /locus_tag="POB34_003195"
     CDS             complement(14285..14512)
                     /locus_tag="POB34_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDI5920087.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
BASE COUNT         3588 a         3744 c         3575 g         3726 t
ORIGIN      
        1 aaagacccgc gtggcgtctt caccctgctt acttttatat tccgccagaa tcggtacaaa
       61 tgcctgggaa aatgcccctt cggcaaagat acggcgtaac aagttaggaa gtttaaaagc
      121 gacgaaaaag gcgtcggttg ccatccctgc gccaaagatt ctggcgacaa ttgcgtctcg
      181 tgcgaagcca agcacacgcg aaaacatggt catcgagctg acggcggcca gcgattttaa
      241 taaattcatc ggtgttctaa tccagaccca cgggcaaaac gcctgcaagg caggcgctga
      301 aagaggcgaa tagtctacct ggattatggt gaattgctac cgccagatgt tacagggttt
      361 attcactcat cgcatcgcgc cagatcttgt caacgatacg ttgcgccagc actgcctgtt
      421 cgccggcggt ttgcggaact gtctgatttt gcacacattc aatgaagtgc cgcgcacagc
      481 cgacaaatcc acgttgctca agcgtactct gccaaccagg aatcggttta tgcactacgc
      541 cctgcccgcg ctcctcacgc cattcgcgca tatccgtaat gtcgatgagc gcaccgtcag
      601 tcacggcctg cacggtttca cgctgacttc cggcacggcg atgcatacag gtggtgatct
      661 gcaaaggacc ggccgaaaaa tggtgctcgg caaacagcat ttcgccagcg tcgttagtca
      721 gtagcgtacc gccatccaga gaggctttgc cgcccgacaa ccacagcgcg gtatccacca
      781 catgcagata atcatccagc aacgtgaaat aaagatcgtg tggcccgacg ctattgctac
      841 gatgtttatc cattcttagc gaggctgcgg tggcgagttg cgtttttaac tcaccgtaga
      901 gtggtgcgaa acgacggtta aaaccgacca tcaacgtcag ttttttccgc gccgccagtt
      961 ccaccagccg ttcagcatcg cgcagatttt ctgccagcgg tttatcgaca cagacatgta
     1021 cccccgcatt gagtaacgta ctgaccacgt caaagtgact ggcggtgctg gaatgtacaa
     1081 aaaccgcatc gcaactggcg gcaaggctgg ataacgaatc ggcataagga atgcgccagc
     1141 tttcacaaat tggcagggct ttcgcgcgcg taggcgacca ggctccttgt aacgtccagt
     1201 cagacgctgc cgccagcacc ggtaaccacg ctttttgagc aatgccgcct aatcccacta
     1261 cgccgatacg taattttttc actttaatct cccaggtggg ccagcaacga atcaagacgc
     1321 tgtttcagtt ctgccacttc gatttccagg gcttcgacgc gggcctgtaa atcaccgtca
     1381 accgcgctcg acatatccgt taccgacggc tgatcctcta cctcaccgct aaaaagatgc
     1441 atgtagcggc tttcacgttt acccggttcg cgggccagac gcaccacaaa aggaccatct
     1501 tcgcgattcg ccagttgttc cagcgtcgac tccacttccg ccatatcgct gaattcatac
     1561 attcgcgcgg cgcggctgcg cagttcaccc ggcgtctggg caccacgcaa taacaacgtg
     1621 gtgattaacg ccacttctgc ggcgctcagt ttcagatcgc caaattctga attacaaaaa
     1681 cgttgctcat atttggtgac ccgattacca aaaccgctca ctgtgcgtag ataatgacgt
     1741 ttgaccagat tatccagctg ttcctgcact tcagattcac tcagattcat gaccggttca
     1801 cggttcgttt tctgattaca ggccgtgact acaccattga ctgagagcgg gtattgctcc
     1861 ggcgtggtca cctgcttttc cagcaagcaa ccaatcacac gagcttccag ggcagtaagt
     1921 tgatatttca tgagattctc cttagcggcc gggcgtccag tctggggtag ttaatgccgt
     1981 cagtacgtga tcgcgccatt gtccatcaat caacagatag tctttcgcat agccttcttt
     2041 ttcaaaaccc agtcgcgcca gtaaatcacc gctgcgttta ttgtgcggca tataattagc
     2101 cataatgcga tgaatatgtt gggtgcgctg catataacga atggctgcgg tcagggcttc
     2161 aaacatgagt cctttgccct gccatttttg cccaatcgaa taaccgagat agcaggcatg
     2221 aaaagagccg cgaacaacat tggaaaaatt ggcaacgcca ataatctctt tttcgtccgg
     2281 gtcgaataaa ccaaagtaga aagctgaacc ttgtttatga aattcgttaa tcatccccag
     2341 cctggcctgc cagcctgatg gatagcagtg gctttcgtcg cgcactggct cccagggctt
     2401 gaggaaatgg cgattctctg cgtaataatc cgccagacgc caggcatcac gatcatgcac
     2461 cagacgcacg accagtcggt ctgtggttaa gcgcactttt ggcacgttac tgcgatagcc
     2521 aaacattcta tacctactcc ttcccgtaac gtctttaata cgctgtaagt attactatac
     2581 ctgcgcaacc agaccctgtg aaaagtccaa aatgccattt tctgcattat tcacatattt
     2641 gataaaaact ctctatcaaa gcgacgtttt gataaaaaaa tattgtcccc gaagtgatgg
     2701 gaaaagtctt agcggcaatg cagaatagaa agtgctcatc ttttatttcc cgggagggga
     2761 aatgtcccgc gtatcgcagg cgaggaacct gggtaaatat ttcctgctca tcgataatat
     2821 gctggtcgtg ctggggttct ttgttgtctt cccgctgatc tctatccgct tcgttgatca
     2881 aatgggctgg gccgccgtca tggtcggtat tgctctcggt ctacgccagt ttattcagca
     2941 aggtctgggt attttcggcg gcgcaattgc cgaccgcttt ggtgccaaac cgatgattgt
     3001 taccggtatg ctgatgcgcg ccgccggatt cgccacaatg ggtatcgccc acgaaccgtg
     3061 gctattgtgg ttttcatgcc tgctctcggg actcggcggc acgttgtttg atccgccgcg
     3121 ttcggcgctg gtggtgaaac taatccgtcc acaacaacgt ggtcgttttt tctcgctgtt
     3181 gatgatgcag gacagtgccg gtgcggtcat tggcgcattg ttggggagct ggctgttgca
     3241 atacgacttt cgcctggtct gcgccacagg ggccgttttg tttgtgctgt gtgcggcgtt
     3301 caatgcgtgg ttgttaccgg catggaaact ctccaccgta cgcacgcccg ttcgcgaagg
     3361 catgacccgc gtgatgcgtg acaagcgttt tgtcacctat gtcctgacgc tggcgggtta
     3421 ctacatgctg gctgtacaag tgatgctgat gctgccaatt atggtcaacg acgtggctgg
     3481 cgcaccctct gccgttaaat ggatgtatgc cattgaagcg tgtctgtcgt taacgttgct
     3541 ctaccctatc gcccgctgga gtgaaaagca ttttcgtctg gaacaccggt tgatggctgg
     3601 gctgttgata atgtcattaa gcatgatgcc ggtgggcatg gtcagcggcc tgcaacaact
     3661 tttcaccctg atttgtctgt tttatatcgg gtcgatcatt gccgagcctg cgcgtgaaac
     3721 cttaagtgct tcgctggcgg acgcaagagc tcgcggcagc tatatggggt ttagccgtct
     3781 gggtctggcg attggcggcg ctattggtta tatcggtggt ggctggctgt ttgacctggg
     3841 caaatcggcg caccagccag agcttccgtg gatgatgctg ggaattattg gcatcttcac
     3901 tttccttgcg ctgggttggc agtttagtca gaaacgcgcc gcgcgtcgtt tgctggaacg
     3961 cgacgcctga tttgatactc ccccctgact cctccatact gaaagtgaac tgacagccaa
     4021 atggaggagt cacgtgaagc tatacattta cgatcactgc ccttactgcc tcaaagcccg
     4081 catgattttc ggcctgaaaa atatccccgt cgaattacat gttctgctca acgacgacgc
     4141 agaaacaccc acccggatgg tcggtcaaaa acaggttccc attctgcaaa aagatgacag
     4201 ccgctatatg ccagaaagca tggatatcgt tcactatgtc gataaactcg acggcaaacc
     4261 gttactgacc ggcaaacgtt cccctgccat tgaagagtgg ctgcgcaagg tcaatggcta
     4321 cgccaacaaa ctgctgttgc cgcgttttgc caaatcggca tttgatgagt tttctactcc
     4381 cgccgcgcgc aaatatttcg tcgacaagaa agaggccagc gcgggtaatt ttgccgacct
     4441 gctggcccac tctgacggtc tgattaagaa tatcagcgat gatttgcgtg cgctggacaa
     4501 actgatcgtc aaaccgaacg ccgtgaatgg cgaactttcg gaagatgata ttcagctatt
     4561 tccgctactg cgtaatctga cgctggtagc cggaatcaac tggccaagcc gcgttgctga
     4621 ttaccgcgat aatatggcga aacagacaca aatcaatttg ttatcatcaa tggcgattta
     4681 atcctgaccc gccgcggcag cctgtattcg cgggttgccg ccgttttcag ttttttctgc
     4741 tggcgagttt atcgctggtg acacatgctg cgttgtgtca ggatattgac gcagatttca
     4801 tgtcgaggac taccatgaac aagtttttat ttgccgctgc attgatcgtc agtggcctgc
     4861 tcgttggctg taatcaactc acccaataca ccatcaccga acaagaaatt aaccagtcgc
     4921 ttgcgaaaca taataatttc tcaaaagata tcggtttacc cggcgtggct gacgcccata
     4981 ttgttctgac aaacttgacc agccaaattg gtcgcgaaga gccgaataag gttaccctaa
     5041 ccggagacgc caatctggac atgaactccc tgttcggtag tcagaaagcg accatgaaac
     5101 tgaagctgaa agcgctgccc gtgtttgata aagaaaaagg tgcgatcttc ctgaaagaga
     5161 tggaagtggt cgatgcgacg gtacaaccgg aaaaaatgca aacggtgatg caaacgttgc
     5221 ttccctattt gaaccaggca ttacgcaatt actttaacca gcaacctgct tacgtcctgc
     5281 gcgaagatgg cagtcagggc gaagcaatgg cgaaaaaact ggcgaaaggc attgaagtga
     5341 agccaggcga aattgtcatt ccgtttacgg attaatgacg agggcgcatt cgcgcccttt
     5401 atttttcgtg caaaggaaaa cgtttccgct tatcctttgt gtccggcaaa aacatccctt
     5461 cagccggagc atagagatta atgactgcac catcccaggt attaaagatc cgccgcccag
     5521 acgactggca ccttcacctc cgcgatggcg acatgttaaa aactgtcgtg ccgtatacca
     5581 gcgaaattta tggacgggct atcgtaatgc ccaatctggc tccgcccgtg accacagttg
     5641 aggctgccgt ggcgtatcgc caacgtattc ttgatgccgt acctgccggg cacaatttca
     5701 ccccattgat gacctgttat ttaacagatt cgctggatcc taatgagctg gagcgcggat
     5761 ttaacgaagg cgtgttcacc gctgcaaaac tttatccggc aaacgcaacc actaactcca
     5821 gccacggcgt gacgtcaatt gacgcaatca tgccggtact tgagcgcatg gaaaaaatcg
     5881 gtatgccgct actggtgcat ggtgaagtga cacatgcaga tatcgacatt tttgatcgtg
     5941 aagcgcgctt tatagaaagc gtgatggaac ctctgcgcca gcgcctgact gcgctgaaag
     6001 tcgtttttga gcacatcacc accaaagatg ctgccgacta tgtccgtgac ggaaatgaac
     6061 ggctggctgc caccatcact ccgcagcatc tgatgtttaa ccgcaaccat atgctggttg
     6121 gtggcgtgcg tccgcacctg tattgtttac ccatcctcaa acgcaatatt caccaacagg
     6181 cattgcgtga actggtcgcc agcggtttta atcgagtatt cctcggtacg gattctgcgc
     6241 cacatgcacg tcatcgcaaa gagagcagtt gcggctgcgc gggctgcttc aacgccccaa
     6301 ccgcgctggg cagttacgct accgtctttg aagagatgaa tgctctgcag cactttgaag
     6361 cattctgttc tgtaaacggc ccgcagttct atggcttgcc ggtcaacgac acattcatcg
     6421 aactggtacg tgaagagcaa caggttgctg aaagcatcgc actgactgat gacaccctgg
     6481 tgccattcct cgccggggaa acggtacgct ggtccgttaa acaataaaaa ttgtagcttc
     6541 ctgttgtcat taggttattt tacctgtata aataaccagt atattcaaca gggggctatt
     6601 atgcgaattg aagtcaccat agcgaaaact tctccattgc cagctggggc tattgacgcc
     6661 ctggctggcg aactttcccg ccgtattcag tatgcgtttc ctgataatga aggccacgta
     6721 tcggtacgtt atgccgcagc gaataattta tcggttattg gcgcaacaaa agaagataaa
     6781 cagcgcatta gcgaaattct ccaggaaacg tgggaaagcg ccgatgactg gtttgtcagc
     6841 gaataatatg cagtgatttt tgtttttgcc gggtcgcccc ggctttttta ttttacttac
     6901 aaattttgag tacaaaattt ctacacttcc tcaaaataat tgcacaatat gctaaaaaaa
     6961 tatgaatgac atgctgtttt attgttcata taaatcttaa aagcagaaat ttatctgttc
     7021 ccccctttaa aaattcggtg aataccctta cttattggta tactgaagtt gcttcagata
     7081 aaaacgcatt gaacctcgaa taacgttgtc tagtaacacg aattaggggg ccatgatgga
     7141 aaaaaataat gaagtcattc agactcatcc gctcgtaggg tgggacatca gcaccgttga
     7201 tagctatgat gcgctgatgt tgcgtttgca ctaccagacc ccaaataagt ccgagcagga
     7261 agggactgaa gttggtcaga cgctctggtt aaccactgat gttgccagac aatttatttc
     7321 gatattagaa gcaggaatcg ccaaaattga atccggtgat ttcccggtaa acgagtatcg
     7381 gcgtcattaa catgacatga ccatccaccc aaaaggcacc tcaccggtgc ctttaccatt
     7441 tctgggttgt atatctgcat tcagttaatt actctgctta aaccatgaga gcagagagaa
     7501 aaaaatgaaa tacgatctca tcattattgg cagcggttcc gtaggcgctg ccgccgggta
     7561 ttatgcaacc cgcgccggtt taaacgtgct aatgaccgac gcccatatgc caccgcatca
     7621 acacggcagc caccacggcg atacgcgatt aattcgccat gcttatggtg aaggcgaaaa
     7681 gtatgtcccg ttggtcctcc gcgcgcaaac gctgtgggat gaactttccc gccacaacga
     7741 agatgatccc atttttgtac gctctggtgt cattaacctc ggcccggctg actccgcatt
     7801 tctcgccaac gtcgcccaca gcgccgaaca gtggcaactc aacgttgaaa agctcgatgc
     7861 gcaagggatt atggcccgct ggccagaaat acgcgtcccg gacaactaca tcggcttatt
     7921 tgagactgat tccggttttt tgcgcagcga actggcgatt aaaacctgga tccaactggc
     7981 gaaggaagcg ggctgtgcgc aactgttcaa ctgcccggtc accgcaattc atcatgacga
     8041 tgatggcgta actattgaaa cggttgacgg tgagtatcag gcgaaaaaag cgattgtctg
     8101 cgcgggaaca tgggtaaaag acctgctccc ggagctgcct gtccagcccg tacgtaaagt
     8161 atttgcctgg tatcaggccg atggccgcta tagcgtgaag aataaattcc cggcgtttac
     8221 cggtgaactg cccaatggcg atcaatatta tggttttccg gcagaaaacg acgcgttgaa
     8281 gattggcaaa cataacggag gccaggttat ccattcagcg gatgaacgtg ttccgtttgc
     8341 ggaagtggtc agcgatggtt cggaagcctt cccgttcttg cgcaatgtat tgccgggtat
     8401 cggttgctgc ctgtacggcg ctgcctgcac ctatgataat tcgcctgacg aagattttat
     8461 tatcgatacc ctacccggcc acgataatac actgctcatt accggcctga gtgggcacgg
     8521 ttttaaattt gcgtcagttt taggggaaat agctgccgat tttgcgcaag acaaaaaaag
     8581 cgattttgat ttgacgccat tcaggctttc ccgcttccaa taatcatcat tacggcctcc
     8641 agaatgaggc cgtttttaca ggagtattta atgtgtcggc tgttgcacta tctcatcaat
     8701 aatattcgcg agcatctgat gctttatctt tttctctggg gattgttggc aattatggat
     8761 ctgatctatg tgttttattt ttagttttcc gcactgacat aaactgacaa atatttaatc
     8821 tccctttcat ttagcgtgaa aaattgaatg agattattcc tgcttctaca gcactcacca
     8881 gacatctctg atgctgaacc cactaaactt atgaagattc tttcattatt aattcaaaat
     8941 tattgaaata aaaataaata tcattcaatt tgttattttt gtgttgcaat tcattctccg
     9001 acacgccatt ttctttctct cgcaaattcg tattggatgc acttatgtca ttcacaaata
     9061 cccctgaacg ctatggtgtt atatctgcgg ccttccattg gctaagcgca attattgtct
     9121 acggcatgtt tgctttaggt ctgtggatgg tcacgctcag ttattacgat ggctggtatc
     9181 acaaagcacc cgaactgcat aaaagcatcg gtattttgtt aatgatgggg ctggttattc
     9241 gcgttctgtg gcgtgtcata tctccaccgc cgggtccgct gccgagttat tcgcccatga
     9301 ctcgtcttgc cgcaaaggct ggacatctcg cattgtacct attgctgttc gccatcggca
     9361 tcagcggcta tctgatctca tctgccgatg gtaaaccgat cagcgttttt ggctggtttg
     9421 acgtccccgc gacccttgcc gacgctggcg cacaggcaga ctttgccggt gccctgcatt
     9481 tttggctcgc gtggagcgtc gtagtactgt ccgttatgca cggatttatg gccctgaagc
     9541 atcatttcat cgataaagac gacactctga agcgcatgct gggaaagtcg tcatctgact
     9601 atggagtata aaaaatgaaa aaaagcctgc ttggtttaac cttcgcgtcc ctgatgttct
     9661 ctgccggttc agcggttgcc gccgattaca aaattgacaa agaaggtcag cacgcctttg
     9721 ttaatttccg catccagcac cttggctata gctggttata cggcaccttt aaagatttcg
     9781 acggtacttt tacctttgac gaaaaaaatc cggctgccga taaagtgaat gtgacaatta
     9841 acaccaccag cgtcgatact aatcacgccg aacgcgataa acatcttcgc agtgcagatt
     9901 tcctcaatac cgcaaaatat ccacaggcaa cattcacctc caccagcgtg aagaaagacg
     9961 gtgacgaact ggatattacc ggcgatctga cgctgaatgg cgtaaccaaa cccgtcacgc
    10021 tggaagcgaa attaattggt cagggcgacg acccatgggg tggtaaacgt gcaggcttcg
    10081 aggccgaagg caaaattaag ctcaaggact tcaatatcaa gacagattta ggtccagctt
    10141 ctcaggaagt ggatctgatt atttcagtgg aaggcgtaca gcagaagtaa gagtgaaatg
    10201 ttgccggatg gcacgcatcc ggcaatgata tttattccgt tggatcagga atgcccagtg
    10261 ttgtattcag acgtccacga gacttattaa agatcttatt gccattttca cgtcctgccc
    10321 gacggcgtcg ctgttcttct ggcggtaacg cgctttcttc gcagcaaatc tcactacaac
    10381 aacctttgta tttttccgca catactggac actgaataaa cagcaggtgg cagccatcat
    10441 ttttacagtt ggtatggctg tcgcacggcg cgccgcactg gtggcaatgc gcgataatct
    10501 catccgatat acgttcgccc atccgctcgt caaaaacaaa atttttgcca ataaaacgca
    10561 ccggcaagcc ctgctcgcgc gccttacggg cgtattcaat aattccgccc tcgatatgcc
    10621 agactttgtt gaatccgtta tgtttcatcc aggcactggc cttttcacaa cgaatgccgc
    10681 cggtgcagta catgacgatt tttttatctt tatgtgcctg catcatctcg actgcttttg
    10741 gcagctgctc acggaaggta tctgccggaa tttcgagcgc gttttcaaag tgccccactt
    10801 catactcata gtggttacgc atgtcgataa atagtgcatc gggatcgtca agcatggcgt
    10861 tcacttccgc cgcttgcaga tactcaccaa cattgctggc atcaaagtga ggatcgtcaa
    10921 taccgtcggc aacaatgcga tcgcgtacct tcatgcgcag tacccagaaa gatttcccgt
    10981 catcatccaa cgcgatattc aggcgtaaac cctctaaagc cgggtcgaag gcatagagct
    11041 gcgcgcgaaa tgtttcaaca ttgctcgcag gtacgctgat ttgcgcgtta atgccctcat
    11101 gcgccagata cactcgccca aaaacattca gcgcggtaaa cagctgatat aaagcgtcac
    11161 gggtcgcctt aggatcggcg atgtggaaat acttgtaaaa cgaaatggtg gttcgcggtt
    11221 cgctctcagc caacattttg gcttttagcg cgtcgttgga aatgcggttg tgtaacactg
    11281 gcatggtgta cggttcctgc gagatgggaa agtaaaaatc cgcggcatga tatagcaatt
    11341 atcgataatt aacatccaca cattttacgc tacatttgcg cattaaaaat tatttgttat
    11401 ttacaagcgc ggcaatttcg cccagtcttc agccacaaat tttggttgcg ggcgaaaaaa
    11461 tgcgacaata catacaattg cccgaatagg ttgaaaaaca ggattgatat gacgaatcta
    11521 cccaagttct ccaccgcact gcttcatccg cgttattggt taacctggtt gggtattggc
    11581 gtactttggt tagtcgtgca attgccctac ccggttatct accgcctcgg ttgtggatta
    11641 ggaaaactgg cgttacgttt tatgaaacga cgcgcaaaaa ttgtgcatcg caacctggaa
    11701 ctgtgcttcc cggaaatgag cgaacaagaa cgccgtaaaa tggtggtgaa gaatttcgaa
    11761 tccgttggca tgggcctgat ggaaaccggc atggcgtggt tctggccgga ccgccgaatc
    11821 gcccgctgga cggaagtgat cggcatggaa cacattcgtg acgtgcaggc gcaaaaacgc
    11881 ggcatcctgt tagttggcat ccattttctg acactggagc tgggtgcgcg gcagtttggt
    11941 atgcaggaac cggggattgg cgtttaccgc ccgaacgata atccactgat tgactggcta
    12001 caaacctggg gccgtttgcg ctcaaataaa tcgatgctcg accgcaaaga tttaaaaggc
    12061 atgattaaag ccctgaaaaa aggcgaagtg gtctggtacg caccggatca tgattacggc
    12121 ccgcgctcaa gcgttttcgt cccgttattt gccgttgagc aggctgcgac cacgaccgga
    12181 acctggatgc tggcacggat gtccggcgca tgtctggtgc ccttcgttcc acgccgtaag
    12241 ccagatggca aagggtatca attgattatg ctgccgccag agtgttctcc gccactggat
    12301 gatgccgaaa ctaccgccgc gtggatgaac aaagtggtcg aaaaatgcat catgatggca
    12361 ccagagcagt atatgtggtt acaccgtcgc tttaaaacac gcccggaagg cgttccttca
    12421 cgctattaaa tctcccctgc cggatgcttc agaatggcat ccggcattac cacagcaaat
    12481 ccccctgatt tagcgataaa agctctctgg attgcgcccc ctggaagtcg ggcgcataat
    12541 tagtgtgctt atcttttctt cttatgttca ccgcgcctgg cgcaccaaca gcggattgct
    12601 atgtcaccct gtgaaaatga cacccctata aactggaaac gaaacctgat cgtcgcctgg
    12661 ctaggctgtt ttcttaccgg tgccgccttc agtctggtaa tgcccttctt acccctctac
    12721 gttgagcagc ttggcgttac cggtcactcc gccctgaata tgtggtccgg tattgtcttc
    12781 agcattacat ttttattttc ggccatcgcc tcaccgtttt ggggtggact cgccgaccgt
    12841 aaaggccgaa aactcatgct attacgctct gccctcggca tgggcatcgt gatggtgttg
    12901 atgggactgg cacaaaatat ctggcagttt ttgatcctac gggcgcttct tgggttactt
    12961 ggcggatttg tccccaacgc taatgctctt atcgccacac aagtaccgcg taataaaagc
    13021 ggctgggcgc tgggtacgct ctccacaggc ggcgttagtg gtgcgttgct cggcccaatg
    13081 gctggcggcc tgctcgccga tagctacggc ttacgtccgg tattctttat taccgccagt
    13141 gtgctcatac tctgcttttt cgtcaccctg ttctgcatca gagaaaaatt ccagccggtc
    13201 agcaaaaaag agatgctgca catgcgggaa gtggtgacat cacttaaaaa cccgaaactg
    13261 gtactcagcc tgtttgtcac cacgttaatc atccaggtgg cgacaggctc aattgccccc
    13321 attctgacgc tgtatgtccg cgaactggcg ggtaacgtca gtaacgtcgc ctttatcagt
    13381 ggcatgatcg cctcggtgcc aggcgtggcg gctctgctaa gcgcaccacg actcggcaaa
    13441 cttggcgatc gaatcggacc cgaaaagatc ctgattacag cgctgatctt ttctgtactg
    13501 ctgttgatcc caatgtctta cgttcagacg ccattgcaac ttgggatttt acgttttttg
    13561 ctcggtgccg ccgatggtgc actactcccc gccgtacaga cactgttggt ttacaactcg
    13621 agcaaccaga tcgccgggcg tatcttcagc tataaccaat cgtttcgtga tattggcaac
    13681 gttaccggac cattgatggg agcagcgatt tcagcgaact acggtttcag agcggtattt
    13741 ctcgtcaccg ctggcgtagt gttattcaac gcagtctatt catggaacag tctacgtcgt
    13801 cgtcgaatac cccaggtatc gaactgattt ttcgcctttc atacttgcaa aagcggagaa
    13861 tcagctatcc ttttccctga aacctcatca actcaaaggg agaatcgtga tgaccatgta
    13921 cgcaacgctt gaagaagcca ttgacgctgc acgcgaagaa tttcttgcag acaaccccgg
    13981 catcgacgct gaagatgcga atgtgcaaca gttcaatgcc caaaaatacg ttttgcagga
    14041 cggcgacatc atgtggcaag ttgagttttt tgccgacgaa ggggaagaag gtgaatgttt
    14101 acctatgctt agcggtgaag ccgcgcaaag tgtttttgat ggcgactatg atgagataga
    14161 gatacgccag gagtggcagg aagagaatac attacatgaa tgggacgagg gggaatttca
    14221 gcttgagcca ccgctggata ccgaggaagg acgcgcagca gctgatgagt gggatgaacg
    14281 ttaatcactc atacgggcca tgatgaatgt cgatcggcag cagtaaagta tcaaaaacca
    14341 gagagaatgg cagatcaagg atcgtgacat aacgccaggc ggagtcacgc acatcccatt
    14401 gcacaccggg ataatattgg ttgccatgcc cctgccccgg tatagtgcga ctaataatgc
    14461 tgccacagcc actcagcaag gtcaccatga tgctcaccac aattaaacgc atttgatacc
    14521 tctttcgtaa aaaaataccg gcataacgcc ggtattttta ttggctcgtt gtttttgccg
    14581 gatgcggcgt gaacgcctta tccgacccac aaaatcttgc aaaatcaata aat
//
LOCUS       DAMZET010000105        14482 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.105, whole genome shotgun sequence.
ACCESSION   DAMZET010000105 DAMZET010000000
VERSION     DAMZET010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.105"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..713
                     /gene="gspC"
                     /locus_tag="POB34_003196"
     CDS             <1..713
                     /gene="gspC"
                     /locus_tag="POB34_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HDI5920088.1"
                     /translation="NYFHTTIVKNGQIINQPTNAFQSDFSLAALWRNENHAGVKDANP
                     VAVNQETPKLSIALNGIVLTSNDETSFVLINEGSEQKRYSLNEALESAPGTFIRKINK
                     TSVVFETHGHYEKVTLHPRLPDIIKQPDSESQNVLADYIIATPIRDGEQIYGLRLNPR
                     KGLNAFTTSLLQPGDIALRINNLSLTHPDEVSQALSLLLTQQSAQFTIRRNGVPRLIN
                     VSVGELTGMNGLRHERTQ"
     gene            697..2649
                     /gene="gspD"
                     /locus_tag="POB34_003197"
     CDS             697..2649
                     /gene="gspD"
                     /locus_tag="POB34_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HDI5920089.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNARKPSSSGAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPIFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYHSLSKEKYTRYRQEQQQRIDGKSK
                     ALVGSEDLPVLDENTFNSHAPAPSSR"
     gene            2659..4140
                     /gene="gspE"
                     /locus_tag="POB34_003198"
     CDS             2659..4140
                     /gene="gspE"
                     /locus_tag="POB34_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HDI5920090.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVS
                     PQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHNALIKNGLQKVISGDTSWDEVMRVASATLESEA"
     gene            4137..5333
                     /gene="gspF"
                     /locus_tag="POB34_003199"
     CDS             4137..5333
                     /gene="gspF"
                     /locus_tag="POB34_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HDI5920091.1"
                     /translation="MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNKRLGDVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTTVAIGVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQRTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLNNLEIRQRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            5343..5780
                     /gene="gspG"
                     /locus_tag="POB34_003200"
     CDS             5343..5780
                     /gene="gspG"
                     /locus_tag="POB34_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HDI5920092.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     VVSDIVALENALDMYKLDNHHYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            5788..6297
                     /gene="gspH"
                     /locus_tag="POB34_003201"
     CDS             5788..6297
                     /gene="gspH"
                     /locus_tag="POB34_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HDI5920093.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDVTNTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESQND
                     WDEELSIHLQPFKPDDSNQPQVVILADGQITPFSLLMANAGTGEPLLTLVCSGSWPLD
                     QTLARDTRP"
     gene            6294..6671
                     /gene="gspI"
                     /locus_tag="POB34_003202"
     CDS             6294..6671
                     /gene="gspI"
                     /locus_tag="POB34_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HDI5920094.1"
                     /translation="MNKQSGMTLLEVLLAMSIFTAVALTLMSSMQGQRNAIERMRNET
                     LALWIADNQLQSQDSFGEENTSSSGKELINGEEWNWRSDIHSSKDGTLLERTITVTLP
                     SGQTTSLTRYQSIDNKSGQAQDD"
     gene            6664..7251
                     /gene="gspJ"
                     /locus_tag="POB34_003203"
     CDS             6664..7251
                     /gene="gspJ"
                     /locus_tag="POB34_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HDI5920095.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPLAPLSEAQTL
                     LTVHWYLRNHTLYRAVRTSVDGRKDQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     QTQQYGGLQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            7244..8227
                     /gene="gspK"
                     /locus_tag="POB34_003204"
     CDS             7244..8227
                     /gene="gspK"
                     /locus_tag="POB34_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HDI5920096.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTLSFHSQLQRTRQVNHH
                     LQRQYDIELAEKLALASLTQDVKDNDRQTTLQQYWAQPQQLQLEDGNTVKWQLRDAQH
                     CFNLNALAKISDDPLASPDFPAQVFSALLINAGIDRGNTDEIVQSIADYIDVDDSPRF
                     HGAEDSFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTTELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPREGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKRHLFPYSRYFTLSTESISDEQSQGWQSHIFFNRKQQSAQIYRRTLQLY"
     gene            8242..9405
                     /gene="gspL"
                     /locus_tag="POB34_003205"
     CDS             8242..9405
                     /gene="gspL"
                     /locus_tag="POB34_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HDI5920097.1"
                     /translation="MPESLMVIRSSSTLRKHWEWMTFSADSVSSVHTLTDDLPLESLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDNTQDWHWTVVDK
                     QNESVEVIGIQSEKLSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQKSWLIR
                     SAAHAFNELDEHWLQHLAAQFPPENMLCYGVVPHGVAAANPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGIFRKQKTVSKSGKWLARLAVSCLVLAILSFVGSRSIALWHTLKIEDQL
                     QQQQQETWQRYFPQIKRTHNFHFYFKQQLAQQYPEAVPLLYHLQTLLLEHPELQLMEA
                     NYSQKQKSLTLKMSAKSEANIDRFCELTQSWLPMEKTEKDPVSGVWTVRNSGK"
     gene            9402..9863
                     /gene="gspM"
                     /locus_tag="POB34_003206"
     CDS             9402..9863
                     /gene="gspM"
                     /locus_tag="POB34_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HDI5920098.1"
                     /translation="MIKSWWAEKSTSEKQIVAALAVLSLGVFCWLGVIKPIDTYIAEH
                     QSHAQKIKKDIKWMQDQASTHGLLGHPALTQPIKNILLEEAKRENLAITLENGPDNTL
                     TIHPVTAPLENVSRWLTTAQVTYGIVIEDLQFTLAGNEEITLRHLSFREQQ"
     gene            9863..10540
                     /gene="gspO"
                     /locus_tag="POB34_003207"
     CDS             9863..10540
                     /gene="gspO"
                     /locus_tag="POB34_003207"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase GspO"
                     /protein_id="HDI5920099.1"
                     /translation="MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHF
                     RQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGALFLYFCFVLTLSVIDFRTQLLPD
                     KLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
                     LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLG
                     YLAWISY"
     gene            complement(10569..11045)
                     /gene="bfr"
                     /locus_tag="POB34_003208"
     CDS             complement(10569..11045)
                     /gene="bfr"
                     /locus_tag="POB34_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HDI5920100.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREEG"
     gene            complement(11117..11311)
                     /gene="bfd"
                     /locus_tag="POB34_003209"
     CDS             complement(11117..11311)
                     /gene="bfd"
                     /locus_tag="POB34_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HDI5920101.1"
                     /translation="MYVCLCNGISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVR
                     AAREVMEDELMQLPEFKESA"
     gene            complement(11480..14173)
                     /gene="chiA"
                     /locus_tag="POB34_003210"
     CDS             complement(11480..14173)
                     /gene="chiA"
                     /locus_tag="POB34_003210"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HDI5920102.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDGK
                     IYENAWWVSSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDAKSNDASNPWRYVRAATATEISETSNPQSCTSAPQPSPDVKPAPDVKPAPDVQPA
                     PADKSNDNYAVVAWKGQEGSSTWYVIYNGGIYKNAWWVGAANCPGDAKENDASNPWRY
                     VRAATATEISQYGNPGSCSVKPDNNGGAVTPVDPTPETPVTPTPDNNEPSTPADSGND
                     YSLQTWSGQEGSEIYHVIFNGNVYKNAWWVGSKDCPRGTSAENSNNPWRLVRTATAAE
                     LSQYGNPTTCEIDNGGVIVADGFQASKAYSADSIVDYNDAHYKTSVDQDAWGFVPGGD
                     NPWKKYEPAKAWSASTVYVKGDRVVVDGQAYEALFWTQSDNPALVANQNATGSNSRPW
                     KPLGKAQSYSNEELNNAPQFNPETLYASDTLIRFNGVNYISQSKVQKVSPSDSNPWRV
                     FVDWTGTKERVGTPKKAWPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVVSKD
                     ANTCLPTWGTAYGMQNYAQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDLMQH
                     YYDIVDNLNLKVLDFDIEGTWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTLPIL
                     PTGLTPEGMNVLSDAKAKGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSAIAN
                     LHSQLKGLHPNKSDAEIDAMMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLGMVG
                     IWSIARDLPGGTNLSPEFHGLTKEQAPKYAFSEIFAPFTKQ"
BASE COUNT         3776 a         3701 c         3424 g         3581 t
ORIGIN      
        1 tgaattattt ccatacaact atagttaaaa atggacagat cattaatcaa ccaacaaacg
       61 catttcagtc tgatttcagc cttgccgctt tatggcgtaa cgaaaatcat gctggggtaa
      121 aagatgcaaa tcccgtggca gttaatcagg aaacacctaa gctctctata gcgcttaatg
      181 gcattgtact cacatcgaat gatgagactt cttttgtctt aattaatgag gggagcgaac
      241 aaaagcgata ctccctcaac gaagcccttg agtcagcgcc gggaacgttt attagaaaaa
      301 taaataaaac cagcgttgtg tttgaaactc acgggcatta cgaaaaagta actctccatc
      361 caaggctacc tgacatcatc aagcagcctg attcagaaag tcaaaatgta ctcgctgatt
      421 atattatcgc cacgcctata cgtgatggag aacagatata cgggcttcga ttgaaccctc
      481 gtaagggcct caatgcattt accaccagct tgctacaacc aggggatatc gccctcagga
      541 tcaataatct ttcgctgacc caccctgatg aggtttcaca ggcattaagt ttactgctaa
      601 ctcaacaaag tgctcagttt acaattcgtc gcaacggcgt accccgcttg ataaatgttt
      661 ccgtcgggga acttacagga atgaatggac tgcgtcatga aaggactcaa taaaatcacc
      721 tgctgcttgc tggcagcact actcatgcct tgtgcaggac acgctgagaa cgaacaatac
      781 ggcgcgaact tcaataacgc cgatatccgc cagttcgtgg aaatagtggg tcagcatctt
      841 ggcaaaacga tcctgatcga cccttcggta cagggaacca tttccgtacg cagtaatgat
      901 acgtttagcc aacaggagta ctaccagttc tttttaagta ttcttgatct ttacggttat
      961 tccgtgatca cgctggacaa tggttttctg aaagtggttc gctcagctaa tgtaaaaaca
     1021 tcgccaggga tgattgctga cagttctcgt ccaggcgtag gtgatgagtt ggtcacccga
     1081 attgtaccgc ttgagaacgt tcctgctcgt gacctggccc ccctgctccg ccagatgatg
     1141 gatgcgggta gcgtcggtaa tgttgtgcat tatgaaccct ccaacgttct tattctgacc
     1201 ggtcgtgcct ccaccattaa taaactgatt gaagtcataa agcgcgttga tgtcatcggc
     1261 acagagaagc agcaaattat tcatctggaa tatgcgtcag cggaagatct cgccgagatt
     1321 cttaatcaat taatcagcga aagccacggt aaaagccaga tgccagccct cctctccgcg
     1381 aagattgtgg cggataagcg aaccaactct cttatcatca gtggaccgga aaaagcacgc
     1441 cagcgcatca cttcattact gaaaagcctt gatgtcgaag agagcgagga aggaaatacc
     1501 cgggtttatt acctgaaata tgctaaagcc acgaatctgg tggaagtgct aaccggtgtt
     1561 tccgaaaagc tgaaagatga aaaagggaat gcgcgtaagc cctcctcttc tggcgcgatg
     1621 gataacgtcg ccattaccgc cgatgaacag actaactctc tggtcattac cgctgaccag
     1681 tccgtccagg aaaaactcgc cacggtaatt gcgcgtctgg acattcgccg tgcacaggtg
     1741 ctggttgagg caatcatcgt tgaagttcag gatggaaatg gactaaacct cggcgtgcaa
     1801 tgggcgaata aaaacgttgg cgcacagcaa tttaccaata ccggattacc gatttttaac
     1861 gctgcgcaag gtgtggctga ttataaaaag aatggtggga tcaccagcgc gaatcctgcc
     1921 tgggatatgt ttagcgccta caatggcatg gccgcaggct tcttcaatgg cgactgggga
     1981 gtactgctta ccgcgctggc cagtaacaat aaaaatgaca tcctcgccac cccaagcatc
     2041 gtaacgctgg ataataaact cgcgtccttc aacgtggggc aggatgtgcc ggtgctatcc
     2101 gggtcacaga ccacttcagg ggataacgtc tttaataccg tcgaacgcaa aacggtgggg
     2161 acaaaactca aagttactcc gcaggtcaat gaaggcgacg cggtgttgct cgaaatagag
     2221 caggaagtct ccagcgttga ctcttcctct aactcgacgc tcggcccgac gtttaatacc
     2281 cgtactattc aaaacgccgt gctggtcaaa accggtgaaa cggtggtcct gggcggattg
     2341 ctggatgatt tttctaaaga gcaagtgtca aaggttcctc tgcttggcga tattccttta
     2401 gtggggcaac tcttccgcta tacctccacc gagcgcgcta aacgcaacct gatggtattt
     2461 atccgtccga cgattatccg tgacgatgat gtttatcact cactgtcaaa agagaaatac
     2521 acccgttacc gtcaggagca acaacagcgg atcgacggga aatcaaaagc gctggttggc
     2581 tcggaagatt tgccggtgct ggatgaaaac acgttcaaca gtcacgcccc tgcgccatcg
     2641 tcacggtgag gcattcatat gagaattcac tcaccgtacc ccgccagttg ggcgctggca
     2701 caacgaattg gttatctcta ttcagagggc gagattattt atctcgccga tacgccattc
     2761 gagcggttac tcgatattca acgtcaggtt ggccagtgcc agaccatgac cagcttgtca
     2821 caggctgatt ttgaagctcg gctggaagcg gtattccatc agaataccgg tgagtcgcaa
     2881 cagattgcgc aggatatcga tcaatccgtc gatcttctct cgctttcgga agagatgccc
     2941 gcaaatgaag atctcctgaa tgaagattca gcggcaccgg ttatccgctt gatcaatgcg
     3001 attttgagtg aggccatcaa agaaaccgcc tctgatatcc acattgaaac ctatgaaaaa
     3061 acaatgtcga tccgttttcg catcgacggc gttttgcgga caattttaca gccaaacaaa
     3121 aaactggcgg cactgcttat ctcccgaatt aaggtcatgg ctcgtcttga tatcgccgaa
     3181 aaacgtattc cacaggatgg aagaattagt ttgcgtatcg ggcgacgtaa catagatgtc
     3241 cgcgtatcca cactgccgtc catctatggt gaacgcgccg tactccgcct gctggataaa
     3301 aacagcctcc agctttcatt gaacaacctg gggatgacgg cagcggataa gcaggattta
     3361 gaaaatctca ttcagcttcc gcacggtatt atcctggtga cagggccgac aggctccggt
     3421 aaaagcacca cgctctacgc catcctttcg gcgctgaata ctcccggccg caatattctg
     3481 acggtagaag atcccgtgga atatgagctg gaaggcattg ggcaaacgca ggtgaatacc
     3541 cgtgtggata tgtctttcgc tcgcggcctg cgcgccatac ttcgccagga cccggatgtc
     3601 gtcatggtgg gggaaattcg tgatacagaa accgcgcaga ttgcggttca ggcctcgctc
     3661 accggccatc tggtactctc aacactccac actaacagtg catcaggcgc agtgacccgg
     3721 ctccgcgaca tgggcgtcga atcattcctg ctttcgtctt ccctggcagg gattatcgcg
     3781 caacgtctgg ttcgtcgcct gtgtccgcaa tgccgacaat tcacgcccgt atcaccccaa
     3841 caagcgcaga tgtttaaata tcatcagctc gcggtgacaa caattggcac tcccgtaggc
     3901 tgccctcatt gccatcaatc cggctatcag gggcgcatgg cgatccacga aatgatggtg
     3961 gtgacgccgg aattacgggc cgctattcat gaaaatgtgg atgaacaagc actggagcga
     4021 ctagtccggc aacaacacaa tgccttaatc aaaaatggcc tgcaaaaagt gataagcggt
     4081 gacacctcct gggatgaggt tatgcgcgtc gccagtgcca cgctggagag cgaagcatga
     4141 attatcgcta tcgcgccatg acccaggatg gtcaaaaatt gcaagggatc attgatgcta
     4201 acgatgaacg tcaggcacga ctgcggctgc gtgaagaagg gcttttcctg ctggatattc
     4261 gcccccaaaa aagttcggga gtaaaaacac gtcgcccgag gatcagccat agtgaactga
     4321 cgcttttcac ccggcagttg gcaaccttaa gcgcagcggc attacccctg gaagagagcc
     4381 ttgccgtaat cggtcaacaa agcagtaata aacgactggg tgacgtgtta aatcaggtac
     4441 gcagcgccat ccttgaaggg catccccttt ccgatgcatt acagcatttt cccacgcttt
     4501 tcgattcgct ctatcgtacc ctggtaaaag cgggcgaaaa gagcgggctg ctggccccgg
     4561 tgttggaaaa gctggctgat tacaatgaaa accggcagaa aatccgcagc aagctcattc
     4621 agtcactgat ctacccctgt atgctcacta cggtggcgat tggggtcgtg attattctcc
     4681 tcactgctgt cgtgcccaaa attaccgaac agttcgtgca tatgaagcag caactgccgc
     4741 tgagtacacg cattctttta ggtctgagcg acacgttgca acgtaccggc ccgacattat
     4801 tagcgacagt gtttattgtc gctgtaggtt tctggctctg gttaaaacgc ggcaataacc
     4861 gccaccgttt tcatgccatg ttgctgcgcg ttgcgctcat cggcccgctg atttgcgcca
     4921 ttaacagcgc acgctatctc cgcactttaa gtattttgca atccagcggc gtccctctgc
     4981 tggatgggat gaatttgtcc accgaaagcc tcaacaacct tgaaattcgc cagcgtctgg
     5041 caaatgcggc agagaacgtt cgccagggta acagcattca tctttcgctg gaacaaaccg
     5101 caattttccc gccgatgatg ctctacatgg tggcctctgg cgaaaaaagc gggcagctcg
     5161 gcacattaat ggtcagagcc gcagataacc aggagacact ccaacaaaat cggatcgcct
     5221 taacgctctc catcttcgag ccagcactca ttattacgat ggcactgatc gtcctgttta
     5281 ttgtcgtgtc ggtactccaa cctcttcttc aacttaactc aatgattaat taaggaaagc
     5341 atatgcgcgc aacggataag caacgcggtt ttacattact ggaaattatg gtggtgatcg
     5401 tcattatcgg cgtacttgcc agcctggtgg ttcctaacct aatgggcaat aaagaaaagg
     5461 cggataagca aaaagtcgtc agcgatattg tggcgctgga aaacgccctt gatatgtaca
     5521 aactcgacaa tcatcactat ccaaccacaa atcaggggct tgaatcctta gtagaagcgc
     5581 cgacactgcc accgctggcc gcaaactata acaaggaagg ttatatcaag cgtctgcctg
     5641 ccgatccctg gggcaatgat tatgtcctcg ttaatcctgg tgaacatggt gcatacgatc
     5701 tgctttcagc agggcccgat ggtgaaatgg gaaccgagga cgacatcacc aactggggtt
     5761 tgagcaagaa gaaaaagtaa gtcagcgatg aatcagcaac gcgggtttac cctgctggag
     5821 atgatgctgg tgctggcgct ggtcgctatc acggcaagcg tggtgctctt cacatatggc
     5881 cgggaggatg tcaccaacac tcgtgcgcgc gaaacagcgg cgcggtttac cgctgcactt
     5941 gaattagcca tcgaccgcgc cacgctcagt ggtcagcctg tcggtatcca tttctctgat
     6001 tcagcatggc gcattatggt accgggcaaa acaccatcag cctggcgctg ggttccatta
     6061 caagaagatg ccgcagacga gagccaaaat gactgggatg aagaactctc aattcatctt
     6121 cagccattta aacctgatga cagcaaccag ccacaggttg tgattctggc cgacggacaa
     6181 atcacgccct tctctttgtt gatggctaac gccgggaccg gtgagccgct cctgacgtta
     6241 gtctgttcag gctcatggcc cctggatcaa acgctcgcca gagatacccg cccatgaaca
     6301 aacaatcagg gatgacactg cttgaagtct tactggcgat gagtattttc accgctgttg
     6361 ctctgacttt aatgagcagt atgcaagggc aacgaaatgc catcgagcga atgcgtaacg
     6421 agacgctggc attgtggata gcggataacc agcttcagtc acaagattca ttcggcgagg
     6481 aaaacacgtc cagttctggc aaagagttaa taaatggcga agagtggaac tggcgcagcg
     6541 atatccactc aagcaaagac ggcacgcttc tggaacgcac cataacggtg acattaccca
     6601 gcggtcagac aacctcgctt acgcgttatc aaagcatcga taataaatcc gggcaggcac
     6661 aagatgatta atcgccagca gggtttcaca ttgctggagg tcatggcggc gctggcgatt
     6721 ttctcaatgc ttagcgtgct ggcgtttatg attttttcgc aagcttccga gttgcatcaa
     6781 cgctcacaga aagaaatcca gcagttcaat cagctacagc gcaccatcac cattcttgat
     6841 aacgatctcc tgcaactggt cgcccggagg aatcggagta cggacaaaat catggtgttg
     6901 ggtgaagaag ctatttttac cacgcaaagt cgcgatccgc tggctcctct cagtgaagca
     6961 caaaccttac tcactgttca ctggtatctg cgaaatcata cgctctaccg tgctgttcgt
     7021 acttctgtgg atggcaggaa ggatcaaccc gcccaggcga tgctggaaca tgtcgagagc
     7081 tttcttctgg aaagtaacag cggggaaagc caggaacttc ctctctcagt cacgttacat
     7141 ctgcagactc agcaatatgg ggggttacag cgccgttttg cattacctga gcagttagcg
     7201 agagaagagt cgccagctca gacgcaggcc gggaataata accatgaata atgaacaacg
     7261 cggcgtcgcg ctgctcattg tactcatgtt actggcgctc atggctgctc tggcagcaga
     7321 catgacgctc agttttcaca gccagcttca gcgaacccgc caggtaaacc atcatctgca
     7381 acggcaatat gatattgaac tggcggagaa acttgcgctt gccagcctca cccaggacgt
     7441 taaagataac gacagacaga ccacgttgca gcagtactgg gcacagccac aacagttgca
     7501 gctggaagat ggcaatacag tgaagtggca gttacgcgat gcccagcact gttttaatct
     7561 caatgcgtta gcaaaaatct ctgacgaccc gctggcttcc cctgatttcc ccgcccaggt
     7621 attcagtgcg ctgctgatta acgccggtat cgatcgcgga aacaccgatg agatcgtgca
     7681 atcaatcgct gactatattg atgtcgatga ctcaccacgc tttcacggcg cagaagatag
     7741 cttttaccaa agccagacgc cgccccggca tagtgccaat cagatgcttt ttctgactgg
     7801 cgaattacgt cagataaaag gcataaccga aaacatctat cagcggctta tcccgtatgt
     7861 ctgcgttctc ccgaccacgg aacttagcat caatcttaat atgctaacgg agaacgatat
     7921 tccgctcttc agagcgttat tcctcaataa catcacggat gctgacgctc gggtcttatt
     7981 gcaaaaaagg ccgagggaag gctggctaac cacggatgca tttctttact gggcgcagca
     8041 ggatttctcc ggtgtgaagc cactggttgc tcaggtgaaa aggcatctct ttccctatag
     8101 ccgctatttt acgctgagta cggaaagtat cagcgacgaa caatcccagg gatggcaaag
     8161 ccatattttt ttcaaccgta aacagcagag cgcccaaatc tatcggcgta cgctgcaact
     8221 ctactaatca taaggatcgt gatgcctgaa tcgttgatgg tcatacgttc ttcctcgacg
     8281 ctgcgtaagc attgggaatg gatgacgttc tccgcagata gcgtatcttc ggtacatacg
     8341 ctcacggacg atcttccgct ggagtcgctg gccgatcagc ccggtgccgg aaatgttcat
     8401 cttttgatcc caccagaggg ccttttatat cgttccctca cgctgcccaa cgccaaatac
     8461 aaactcaccg cacaaacatt acaatggctg gcagaggaga cgttacccga caacacccag
     8521 gactggcact ggacggttgt cgataaacaa aacgaaagtg tcgaggtgat aggcattcag
     8581 tcggagaaac tcagtcgcta tcttgagcgt ttacatactg ccggactcaa cgtgacgcgg
     8641 gttttgccag atggctgcta tcttccctgg gaagtagata gctggactct ggttaaccag
     8701 caaaaaagct ggctcattcg cagtgccgca catgccttca acgaactgga tgaacactgg
     8761 ttacagcatc ttgctgccca gtttccgcct gagaatatgc tttgttatgg tgtagtacct
     8821 cacggcgttg cagcggccaa tccacttatc cagcatcctg aaataccatc actgagtttg
     8881 tattctgccg atattgcatt ccagcgctat gacatgctgc atggtatttt tcgtaaacag
     8941 aaaactgtaa gcaaatcggg aaaatggctc gcccgcctgg cagtaagctg tctcgttctc
     9001 gccattcttt cttttgttgg cagccggagc atcgcgctct ggcatacgct taagattgaa
     9061 gatcaacttc agcaacaaca gcaagaaacc tggcaacgtt atttcccgca gatcaaacgc
     9121 acccacaact ttcattttta ctttaagcag caactcgctc agcaatatcc tgaagctgta
     9181 ccgttgctct atcatttaca aacacttctg ctggaacacc ctgaactgca gttaatggag
     9241 gcgaactata gccagaagca aaagtcgctg accttaaaaa tgagtgctaa gagtgaagcc
     9301 aatatcgatc gtttctgtga gttaacacag tcctggcttc cgatggaaaa aacagaaaaa
     9361 gatccggtca gtggtgtatg gacagtaagg aactcaggga aatgattaaa tcatggtggg
     9421 cagaaaaatc cacatcggaa aaacagatcg ttgcggcact ggcggttctc tcgctcggcg
     9481 ttttttgttg gcttggggtg ataaaaccga tagataccta cattgcagag catcagtccc
     9541 acgcgcaaaa aattaagaag gacatcaaat ggatgcagga tcaagcgagc acccatggtt
     9601 tactcggtca tcctgcgctt acgcagccca tcaaaaatat ccttctcgag gaagcaaaac
     9661 gtgagaattt ggctatcacg ctggaaaacg gcccggacaa tactctgaca attcatcctg
     9721 tcacagctcc cctcgaaaac gtctcccgct ggctcactac ggcacaggta acatacggta
     9781 ttgttattga agaccttcag tttactcttg ccggaaacga agagatcacc ctcaggcatt
     9841 tgtccttcag ggagcaacaa taatgacaat gctactgccg ctcttcattc tggttggttt
     9901 tattgcaggt tattttgtta atgccatcgc ctatcacctc tcgccgttgg aggataaaac
     9961 ggcgttaacg tttcgccagg tattggttca tttcaggcaa aaaaaatatg cctggcatga
    10021 tacagtgccc ctgatacttt gtgttgctgc tgctatcgcc tgcgccctgg cacctttcac
    10081 gcccatcgtg actggcgcac tctttctcta tttctgtttc gtactcacgc tcagtgttat
    10141 tgattttcgc actcagctcc tgcccgacaa actcacctta ccgctgctct ggcttggctt
    10201 ggtatttaat gcgcagtatg gattaattga tttacatgat gcggtttacg gcgcggtagc
    10261 gggctatggg gtgctgtggt gtgtttactg gggcgtctgg ttagtttgtc acaaagaggg
    10321 attgggctac ggtgatttca agctactggc cgctgcaggc gcatggtgtg gctggcaaac
    10381 gttgccaatg atactgctga ttgcctcgct gggtggcatt ggttacgcca tcgtttcaca
    10441 acttctgcaa cgccggacta taaccactat cgcattcggc ccgtggcttg cgctcggcag
    10501 catgataaac ctggggtatc tggcctggat ctcttattaa ccgggagggt tctccctccc
    10561 gacacggctc aaccttcttc gcggatctgt gcttgcagat aattttgcag gcccatcttc
    10621 tgaatcagat caagttccgt ttccagccag tcgatatggc cttcttcatc acgcaaaatt
    10681 tctatcatca tatcgcggct gacgtaatca tgaacgctat cggcataacc aattgcctca
    10741 cgcaaattct tcgcgccatc cagctcaagt gccagatcag aacgcagcat ttcctcaaca
    10801 tcttcaccaa tgttcagttt gcccaggtcc tgtaagtttg gaagaccttc cagaaaaaga
    10861 atgcgctcaa tataacgatc ggcgtgtttc atctcatcaa tggattcatg atactccaca
    10921 tcattgagac gtttgagacc ccagttttta aacattcggg catggagaaa gtactgattg
    10981 attgcgacaa gctcatttcc caacagtttg ttgagataat ttataacttt agtatcacct
    11041 ttcatttttt gactccttcg cttccactct ttgaagagta gaacggctta cgagtaagtc
    11101 aaaaaacaca ctccgtttat gcggactcct taaactccgg cagctgcatt aattcatcct
    11161 ccatcacttc acgcgcggcc cggacacatt taccgcactg attgccgacc ggaataaatt
    11221 tttttaattg ctggaacgag tgaggggaaa actggcgcac agcctgacga atttttttat
    11281 cgctgatacc attacaaaga caaacgtaca tagagactcc cgtatacttt cttcaaaaat
    11341 aaatgaaaat agttcttatt tcaattacgg aaagactctt ttttaagcaa agaatagaaa
    11401 tatgaataaa aataatgggc taccaaaagt agcccattga caaaaaatgc ggcgatactg
    11461 gaaggtatcg ccaacacatt tattgcttag taaacggcgc gaagatttcg ctaaatgcgt
    11521 acttcggtgc ctgttcttta gtcaggccgt ggaattccgg agacaggtta gtgccgcccg
    11581 gcaggtcgcg cgcgattgac cagataccaa ccataccgag attacgcttc tgcgcatcct
    11641 gcatgaccag acgagcatca gagagataga acacctcgcc ctgaacgtcg ttcacgccaa
    11701 ccatcggcgt ggtacccatc atagcgtcaa tttctgcatc gctcttattg ggatggaggc
    11761 ctttcaattg tgaatgcagg ttggcaatcg cagacgttgc acacttaccg tgaatgttct
    11821 ggccttcggt atttgcagac tgacaaatcg cgttaccgta gtccattgtc atcacgttca
    11881 cacccgccag ctcaacacct ttcgctttgg catcgctcag gacattcatc ccttccggcg
    11941 tcaggccagt cggcagaatt ggcaaggtgt accagatagc aatatctttg ccttctgact
    12001 tccatttatc ctgcactttc ttcacagcaa ggttacgacg ttcaatagat gcctgatccg
    12061 caacccaggt gccttcgata tcgaagtcca ggactttgag gttcaggtta tcaacgatgt
    12121 cataataatg ctgcatcaga tcgtctacgt tcttacagga agcagccagc ggagcgttgt
    12181 tagcaccacc gatagacagc atcacatcgc cgccagcctc acgcagagct ttgattttgc
    12241 tgtactgagc gtaattctgc ataccataag cggtacccca tgtcggcaga caggtgttcg
    12301 catctttact caccacaaac gccagcgtga agtggttgac gttatgattc ttagccagcg
    12361 cagccaaatc cgggatcgta ttcagcgtaa agtcaacata cggtgcataa acgtgtttcg
    12421 gccatgcttt cttcggcgta cctacacgtt ctttggttcc ggtccagtca acaaaaacac
    12481 gccacgggtt gctgtcagaa ggagaaactt tctgcacttt actctgagaa atgtagttca
    12541 caccgttaaa gcgaatcagc gtatcgctgg cataaagcgt ttctggatta aactgcggcg
    12601 cattattcag ctcttcgttg ctatagctct gagccttacc taacggcttc cacgggcggc
    12661 tattgctacc ggtggcgttt tggttcgcaa ccagagcagg gttgtcactt tgcgtccaga
    12721 acagcgcttc ataagcctgc ccatcaacaa caacgcgatc acctttcacg tacacagtgg
    12781 atgcggacca tgctttcgcc ggttcgtatt tcttccacgg gttatcgccg cccgggacaa
    12841 agccccatgc gtcttgatcg acagaagttt tataatgtgc atcgttataa tctacgatgc
    12901 tgtccgcgct gtacgctttg ctggcctgga aaccatccgc aacaatgacg ccgccgttat
    12961 caatttcaca ggtagtcggg ttaccgtact gactcaattc cgcagcggta gctgtacgca
    13021 cgagacgcca tgggttattg gagttttcag cgctggtacc ccgtgggcaa tctttagacc
    13081 caacccacca ggcgttcttg taaacattac cattgaaaat aacatggtaa atttcgctac
    13141 cttcctggcc gctccacgtt tgcaatgagt aatcgttacc gctatccgct ggtgttgatg
    13201 gctcgttgtt atccggggtt ggggtcaccg gtgtttccgg agttggatca accggagtca
    13261 cagcaccgcc attattatcc ggcttaacgg aacaggagcc agggttacca tactggctga
    13321 tttctgttgc cgttgccgcg cgaacataac gccatgggtt actggcatcg ttttctttcg
    13381 catcgcctgg gcaatttgcc gcgcctaccc accaggcgtt cttataaatg ccgccgttat
    13441 agataacgta ccatgtagaa gaaccttcct gacctttcca ggctactaca gcatagttgt
    13501 cgtttgactt atcagctggg gctggctgaa catcaggagc cggtttaacg tccggtgccg
    13561 gtttcacatc cggtgaaggc tgtggtgctg aagtacagga ctgtggatta ctggtttctg
    13621 agatttccgt tgccgtagcg gcacgaacat aacgccatgg gttgctggca tcattgcttt
    13681 tcgcatcacc cggacagtta gaagaggcta cccaccaggc atttttatat accgcaccat
    13741 taaagacgac ataccaggtc tgaccgccct gctgtttatt ccaggcaact acagaagaat
    13801 tagacggaac agtgccctgt gctggtgtag ccgaaccgcc attagccggc gtattggatg
    13861 caggcgtatt tgaatttgaa gaagatgagc tgccgctctt ttcgcaggaa agtgtattgc
    13921 caaactgact aatttcagca gctgttgcgg tacgctttaa acgccacggg ttagttgcat
    13981 catttgcttt cgcttttccc gggcaatttg tagaagaaac ccaccaggca ttttcataaa
    14041 ttttgccatc gaaaataacc tgatatttat taccaccttg ttggttattc catgcttcca
    14101 ttgccaatgc tggcagagcg gaacacacca gccccatacc aatcatagat ttagtaaata
    14161 tatttaattt cattataatc ccttgtgacg taaaaactgc aaaacaaaag tcctacgcag
    14221 gaattattta cgttttacga gaacccttca atatgaatta aagtgcggca ttaaaaataa
    14281 gaaaaaagcc tgacaaatga agaattttaa aaacagaaac attcatattt aaaatattaa
    14341 attgaattga tattttaaat atgaataatt tattcgttct gacagtacga attagatata
    14401 cagccaacaa atgcaaaaag ggcgccgaag cgcccttttc aattcaaaac taattaacgt
    14461 gtaattagcc cagaacttta gc
//
LOCUS       DAMZET010000106        14455 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.106, whole genome shotgun sequence.
ACCESSION   DAMZET010000106 DAMZET010000000
VERSION     DAMZET010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.106"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..503)
                     /gene="rppH"
                     /locus_tag="POB34_003211"
     CDS             complement(<1..503)
                     /gene="rppH"
                     /locus_tag="POB34_003211"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HDI5920103.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNA"
     gene            1188..1877
                     /gene="mutH"
                     /locus_tag="POB34_003212"
     CDS             1188..1877
                     /gene="mutH"
                     /locus_tag="POB34_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HDI5920104.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            1946..2659
                     /locus_tag="POB34_003213"
     CDS             1946..2659
                     /locus_tag="POB34_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDI5920105.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            2797..3015
                     /gene="ygdR"
                     /locus_tag="POB34_003214"
     CDS             2797..3015
                     /gene="ygdR"
                     /locus_tag="POB34_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HDI5920106.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            3123..4163
                     /locus_tag="POB34_003215"
     CDS             3123..4163
                     /locus_tag="POB34_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HDI5920107.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(4195..5388)
                     /gene="lplT"
                     /locus_tag="POB34_003216"
     CDS             complement(4195..5388)
                     /gene="lplT"
                     /locus_tag="POB34_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HDI5920108.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(5381..7540)
                     /gene="aas"
                     /locus_tag="POB34_003217"
     CDS             complement(5381..7540)
                     /gene="aas"
                     /locus_tag="POB34_003217"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HDI5920109.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRMPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            8126..9157
                     /gene="galR"
                     /locus_tag="POB34_003218"
     CDS             8126..9157
                     /gene="galR"
                     /locus_tag="POB34_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HDI5920110.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(9164..10426)
                     /gene="lysA"
                     /locus_tag="POB34_003219"
     CDS             complement(9164..10426)
                     /gene="lysA"
                     /locus_tag="POB34_003219"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDI5920111.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDVGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            10548..11483
                     /gene="lysR"
                     /locus_tag="POB34_003220"
     CDS             10548..11483
                     /gene="lysR"
                     /locus_tag="POB34_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HDI5920112.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(11470..12162)
                     /gene="ygeA"
                     /locus_tag="POB34_003221"
     CDS             complement(11470..12162)
                     /gene="ygeA"
                     /locus_tag="POB34_003221"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDI5920113.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(12291..13709)
                     /gene="araE"
                     /locus_tag="POB34_003222"
     CDS             complement(12291..13709)
                     /gene="araE"
                     /locus_tag="POB34_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HDI5920114.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(14024..>14455)
                     /locus_tag="POB34_003223"
     CDS             complement(14024..>14455)
                     /locus_tag="POB34_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI5920115.1"
                     /translation="DVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIR
                     VPSYTASKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDR
                     IPAGRWGLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
BASE COUNT         3709 a         3838 c         3498 g         3410 t
ORIGIN      
        1 gcgttttgtg gttttggcgt attttcctgc agtgacatca ccacactcgc gaactctttc
       61 attaccctac ggtagacatc acgtttaaat gacaccacct gtctgaccgg ataccagtaa
      121 cttacccatc gccagccgtc aaactctggt gtactgctgg tttgcatatt gatttctgca
      181 tcgccgctca ccagctgcaa gagaaaccat ttttgttttt ggccgataca aaccggcttc
      241 gtgtcccaac gcaccaaacg tttcggtaat ttgtagcgca accagttacg cgttgaagca
      301 aggattcgaa cgtctttgcg gcttaatcct acttcttcaa acaattcacg gtacatcgcc
      361 tgctctgcgg attctccggg gttgattccg ccttgcggaa attgccagga gtgctgacca
      421 aatcgccggg cccacattac ctgcccctgg cgattacaaa tcacgatacc tacgtttggg
      481 cggtagccat cgtcatcaat caccggacta cctcaaaata aagctttata tacgaatgat
      541 tgtttcatac tccaggaaga cggtaaacca ctctctgcag ggcattacac actaataaca
      601 attgaataac tcacagttat gtgcagagtt ataaacagag gaaggggtgg atagccgttt
      661 ttattcacct tttctgtgga tagagttgtg aagaactacg gaattactac gggaaaaccc
      721 ggagcattct gaataagccc gatatagaca ttttaaaata ttcattatta gtcaatgcat
      781 tacatgtaat ttgagtaatg aaaaagagta attcgtgacc caggtcacac ctctcattta
      841 cgggttggcg aaagatcaac caatgccgta tttatccaca gaatgtgcca ctaagttaag
      901 cactgaacca ctaaaaactg gagtttcgtc gcacgtcaag gctgtaaatg gaaacagtag
      961 tggaggtttt tcacagttat cccagctttc tgtggataac atggtgtaag atcctgttta
     1021 ttttcagtga ccagatttgg aaaacccgtt gcagtgttgc gcaactcgat taccggcagc
     1081 ctaaaaagca atataaatca gggaattgaa caacacatgt ggaaaaagtt acactgcgaa
     1141 tattcggcac ataattgctg tttgtttttt aatcaaggta tcatgacatg tcccaacctc
     1201 gcccactgct ctctcctccc gaaactgaag aacagttgtt agcgcaagca cagcaacttt
     1261 ctggttatac attgggagaa ctggcggcac ttgccgggct tgttacgcca gagaatttaa
     1321 aacgcgataa gggctggatt ggcgtgttac tggagatctg gctaggtgcc agcgcaggga
     1381 gtaaacctga gcaagatttt gccgctctgg gcgtggaact taaaactatc cctgtggata
     1441 gtcttggtcg tccgctggaa acaacattcg tttgtgttgc cccgttaacg ggtaatagcg
     1501 gggtgacctg ggaaaccagc catgtgcgcc acaagctcaa acgcgtactg tggataccgg
     1561 ttgaaggcga gcgcagcatc ccgctggcgc agcgtcgcgt aggatcaccg ttgctgtgga
     1621 gcccgaatga agaggaagac cgacagctac gcgaagactg ggaagaatta atggatatga
     1681 ttgttctcgg tcaggttgag cggatcaccg ctcgtcacgg ggaatattta cagataagac
     1741 cgaaagcagc gaatgcgaaa gcgcttaccg aagccattgg tgcccggggc gaacggattc
     1801 tgacgctgcc acgcggcttt tatttgaaga agaatttcac cagtgcacta ctggcccgtc
     1861 attttctgat ccagtagcca tcgctttgac ctgccgcttt ccgggcatat aattaccgct
     1921 tcattttttt ggcagggctt tttagatgtt atttgcatgg ataaccgatc ctaacgcctg
     1981 gcttgcgctc ggtacgctga cgctgctgga gatcgttctt gggatcgaca atattatttt
     2041 cctttctctg gtggtggcaa agcttcccac agcacaacgt gctcatgcgc gccgtctggg
     2101 gttggcggga gccatggtta tgcgtctggc gctgctggca tcaatcgcct gggttacgcg
     2161 cctgacaaat ccgcttttta caatattcag tcaggaaatt tctgcccgtg atttgattct
     2221 gcttctgggt ggcttgttcc ttatctggaa agccagcaag gaaatccacg aatccattga
     2281 aggtgaagaa gaagggctga aaacacgcgt ttcatcattc ctcggcgcta tcgtgcagat
     2341 tatgctgctg gatattatct ttagcctcga ctcggtgatt acggctgtgg gtctgtcaga
     2401 tcacctgttt attatgatgg cagccgtggt gattgccgta ggcgtgatga tgttcgccgc
     2461 gcgctcgatt ggtgattttg tcgaacgcca tccttcggta aaaatgctgg cgctctcttt
     2521 cctgattctg gtgggcttta ccctgattct ggaaagtttc gacatccacg taccgaaagg
     2581 ttacatctac ttcgcgatgt tcttctctat tgcggttgaa agcctcaact tgattcgcaa
     2641 caaaaagaat ccgctctgat actccgtacg ctctcctgcg ggagggcgta ctcactcctt
     2701 ttaccctcac agtttaagat tttcctgctt tcaagatata taacgtcggt ttataaacag
     2761 actattatca taggtaggcc aaatgaggat aaccggatga aaaaatgggc agtaataatt
     2821 tctgcagtcg gactggcgtt tgctgtttcc gggtgttcca gtgattacgt catggcgacc
     2881 aaagatggcc gtatgatttt gaccgatgga aaacctgaaa ttgatgatga taccgggctg
     2941 gtgagttatc acgatcagca aggtaacgcg atgcaaatta accgtgatga tgtttcgcaa
     3001 attattgaac gttaacaaat aaggtcagca tccggctggc cttaagattt ttctctttcc
     3061 cttttccctt ccctctgcca tttttatatt ccttatgtcg tgattataaa aaggaaacgg
     3121 ctatgcaata tcaccgtata ccccacagtt cgctggaagt cagcacgctg gggcttggca
     3181 cgatgacgtt tggtgaacag aacagcgaag ccgacgccca cgcacaactc gactatgccg
     3241 tcgctcaggg cattaacctt atcgacgttg ccgaaatgta cccagtacct ccgcgccccg
     3301 aaacgcaagg gttaaccgaa acctacgtcg gcaactggct ggcgaaacat ggcagccgcg
     3361 aaaagttaat tatcgcctcc aaagtgagcg gaccgtcgcg caataatgac aagggcatcc
     3421 gcccggatca ggcgctggat cggaagaata tccgcgaagc gctgcatgac agcctcaagc
     3481 gcctacagac tgattacctc gatctttatc aggtgcactg gccgcagcgc ccgaccaact
     3541 gcttcggcaa actcggttat agctggacgg attctgcgcc tgcggtttcg ctgctggata
     3601 cgctggacgc actggcagag taccaacgcg cgggaaaaat tcgttatatc ggcgtgtcga
     3661 acgaaactgc atttggcgta atgcgctacc tgcatctggc ggacaaacac gatctgccgc
     3721 gtattgtcac cattcagaac ccttacagtc tgttaaaccg cagttttgaa gtaggtctgg
     3781 cagaagtcag ccagtatgaa ggggtcgaac tgctggccta ttcgtgcctg ggtttcggca
     3841 cgctgaccgg gaaatatctc aatggtgcaa aacccgctgg cgcacgtaat acgctcttta
     3901 gtcggttcac ccgctatagc ggtgagcaaa cgcaaaaagc cgtcgcggcg tatgttgata
     3961 tcgccagacg tcatggcctg gaccctgctc agatggcgct cgcgtttgta cgccgtcaac
     4021 cgtttgttgc cagcactctg ctgggcgcaa ccacgatgga tcagctgaaa actaacatcg
     4081 aaagtttgca tctggagtta agcgaagacg tattagctga aattgaagcg gtgcatcagg
     4141 tttatactta tccggcacca taagattaac gccggttaaa accggcgtta aatattaatg
     4201 acggcgctgc cagatccaca gcgccgttat tgccagcgca aacagcgcac cgaagccaat
     4261 gccaatgggc acgaccggga tgcctatcat taccgccagc gagtaaatgc ccagcatcaa
     4321 caacatggcg ctgttttcgc caaggttttg tactgcaatc gcattccccg ccccgacgct
     4381 ttttttaccc cgctcctgta gcaacgcatt gagcggaacg acaaaaaagc cccccatcac
     4441 gccaatcagc atcaacaagg cataggctgg cagcagctcg tgttgcaggg aaaaaatcag
     4501 taccaccacg ccaatcaaaa tcccggctgg catacagcgt gacacggttt ccagcgtaac
     4561 taacttcgct gccgcacctg cgccaaccac gataccaatc gctaccatcg cgttgagata
     4621 ggtgggcgta gcgttatcgg taatgcccag cgccaccggt acccacagca ccaacaggaa
     4681 acgcagcgtg acacccgctc cccagaataa actggtgccc accagcgaaa aacgcgtttc
     4741 accattgcgc catagcgagg tgcaggcatt caggaaactg cgggtcatgt tgatgagatt
     4801 ccaggactgc cccggacgcg ccgccgccag tttgggaatg tagatattgg caacgaccgc
     4861 accaccgtag gccagtgcgc atgcggccag ggcgacgagg acatgccagt cagccagcac
     4921 accaccggct acggaaccga gcaaaatcgc cgctatggta gaagcttcca ttaaaccgtt
     4981 agctttcact aacttactac ccgtggttaa ttcgccgaga ataccgtatt tcgccggtga
     5041 ataggctgca gcaccaacac ccaccagcgt atagccgaga aacggattga taccaaagca
     5101 gatactggct gcgcccagca gcttcaggcc gttggcaaac atcatcaccc ggcctttggc
     5161 gaagctatcc gccacctgcc cgacaaacgg cgcaaaaaga atgtaagcac ctacaaacac
     5221 catttgcagg atgggctggc tccactccgg atagaactgc gctttcagta acgccagagt
     5281 ggcaaacagt agggcattat cgccaaacgc agagagaaac tgcgccacga taaccgcttt
     5341 catccccttc gaccacaacg aagtgttagt gtgcactgac tcactcatcg tgttgttccg
     5401 cttcgtctac ccagcttttc aacgtgacaa agtcaggttt gccgctgcca agtaatggca
     5461 tctgtttcag atagcgaata tcgcgcggta cagcaagctc cggcacgccg tgctcgcggg
     5521 catactgttg caacttatcg cgcgtcagtt cgttatctgt ggtgaaaagc accagtgcct
     5581 cgcctttgct ggcatcgctc ttaatcgcag tggcatggac tttatctggc gaaacaccaa
     5641 gtgccagttg ttccaccatt tccagcgaca ccatttcgcc tgcaattttg gcaaagcgtt
     5701 ttgcgcggcc ctgaatctgc acaaagccct gctcgtcaaa acgcacaata tcgccagtgt
     5761 cataccagcc gcgctccatt tcgccgcgaa cattctcggc ggtgggcact tccagtacac
     5821 ctggcttctc cacccgcaga tagccgttca ttatgttcgg ccctttcagt tgcaggcgtc
     5881 cgccctcttc gataccagga accgacaaca ggcgcgcatc cattcctggt agaatacgcc
     5941 ctaccgtacc gggtttcgcc gccatcggta cgttgataga aacgacaggc gcgcactcgg
     6001 ttacaccata gccttcaagg atgcgcaggc caaatttatc ctgccaaagc tgtttggtac
     6061 tttcttgtag tttttctgcg cctgccacca catagcgtag acgatagaag tcatacgggt
     6121 tggcgaaacg cgcgtagtga ccgaggaaag tcgaggtacc gaacaacacg gtgcaactgc
     6181 ggtcatacac caactccggc acaatgcggt aatgcagcgg gcttggataa aggaacactt
     6241 ctgcacctgt aagcagtggc gtaaacaggc ctaccgtcag cccaaaggag tgaaacagcg
     6301 gtaacgccga cataaagcga tcgttggtgg tgaagtcggc aatcgtttta atctgctcga
     6361 cattcgccag aatgctttta tggctatgga cgacgccttt cggatggcct tcagaaccgg
     6421 aggtaaagag gatcagcgcc tcttcttccg gctgctgttt aacctgtgcc agacgcggca
     6481 tcagcaaatg agcgaagatc catactttgt cggcagtggt gacatctgct tttaaatctt
     6541 ccagatagac ccagcgcacc tgagtaagtt gctccggcag atgccagagt ttgcctttat
     6601 cgagaaactg gcgggaagtg aagatggttt tgatttcagc cgccgtaata gcactggtca
     6661 gcccttttac cccggcagtg tagttcatca ttgcgggcat gcggcgacgg gcgatggccc
     6721 caaaaatcac tgccgcactg atgcctgcat tgggcagcat taagccgatg cgttcgcctt
     6781 caacactgta tttttcaagg atgcgtccaa caaacagcgt tttcgtaagc aatttgcgat
     6841 aggagtctgg ggtaaagttg acgtcttcga cacatttctt cccggctccg aagcggtaca
     6901 ttgcactcag taaagattcg tacagcgttt cacgcgggcg caccgccatt cgcgcttcca
     6961 tcattatttg atgcagcatt tcgccagcga ttttgcgacg gtcacgggca cgcggcgcat
     7021 ccggcatcgc cacctgcgtt ggtggcaaaa tatgcagagt aatttgcggg aacaagcgac
     7081 gtttaaccag acctttcagg cggctgaagt gcgtaagttc cgccccttca atacgcacag
     7141 gaataaccgt tgcaccagac ttcgccgcga caaaacccgc gccatcgtag attttcatca
     7201 gcgagcctgt agtggtgatg cgtccttcag ggaaaatcac cactggtcgg ccctgttcca
     7261 ccagacgtac cagatgttta atagccatag gttgcgtcgg gtcgagagga acaaagtcga
     7321 taaatgattt cagccaacgc atataccact gttggcttat tgaggtgtaa acggcaaaca
     7381 ctggacgcac aggtaaaaac agtccaagca aaatgccatc aataaaagag acgtgattag
     7441 gcgtaattag aacgcgctcg cccttcagtg cctgggtgtc acccgtaacg cgaacgcgat
     7501 acaaaacacg gcacaaattt cgaaaaaagc taaaaagcat tccaactccc tttgctctga
     7561 ttcagtaaaa gcgaatggag ggagattaca cgagataaag aacgcgagcg acagtaaatt
     7621 aggtgcgaaa aaaaacctgc gcatccgcgc aggttggtgc aagagacagg gtacgaagag
     7681 cgtaccgaat aatctcacca atcaatacct ctgggatctt gattgtggtc tgcacgacga
     7741 ctcttcgcca gcgagaaaac gcaaaggaat gaagggaaat gcaacgaggt gtgtaaattg
     7801 tcggttactg ttacagattg atgaccggca aaaaaaaacc tgcgcatctg cgcaggctgg
     7861 tgtaattcat gtgctcaacc cgaagttgac ttcacctatc aatacctctg ggatcaccac
     7921 tttagcaacc tgaagccaaa cgccaccagc ggtcaatcgc aacagcgttt cgcaaagtgt
     7981 aaccaaaggt ttgcaatctc tttttttgtc ttacttattt gcgtttggct cacattccca
     8041 cgatgaaaac acgccacccc ttgaaccaac gggcgttttc cgtaacactg aaagaatgta
     8101 agcgtttacc cactaaggta ttttcatggc gaccataaag gatgtagccc gactggcagg
     8161 cgtttcagtc gccaccgttt cccgcgtcat taataattca cctaaagcca gtgaagcttc
     8221 ccggctggct gtgcatagtg caatggagtc tcttagctat cacccgaacg ccaacgcccg
     8281 tgcgctggcg cagcagacca ctgaaacggt cggtctgatc gttggtgatg tttccgatcc
     8341 gtttttcggt gcaatggtga aagcggtcga acaggtggct tatcacaccg gtaatttctt
     8401 attgattggc aacggttacc acaacgaaca aaaagagcgt caggctattg agcaactgat
     8461 ccgccatcgc tgtgctgcgt tggtcgtcca tgccaaaatg atcccggatg ccgatttagc
     8521 ctcattaatg aaacaaatgc ccggtatggt gctgatcaac cgtatcctgc ctggctttga
     8581 aaaccgttgt attgctcttg acgatcgtta cggtgcctgg ctggcaacgc gtcatttaat
     8641 tcagcaaggt catacccgca ttggttatct gtgctctaac cactctattt ctgacgccga
     8701 agatcgtctg caagggtatt acgatgccct tgctgaaagt ggtattgcgg ccaatgaccg
     8761 gctggtgaca tttggcgaac cagacgaaag cggcggcgaa caggcaatga ccgagctttt
     8821 gggacgagga agaaatttca ctgcggtagc ctgttataac gattcaatgg cggcgggtgc
     8881 gatgggcgtt ctcaatgata atggtattga tgtaccgggt gagatttcgt taattggctt
     8941 tgatgatgtg ctggtgtcac gctatgtgcg tccgcgcctg accaccgtgc gttacccaat
     9001 cgtgacgatg gcgacccagg ctgccgaact ggctttggcg ctggcggata atcgccctct
     9061 cccggaaatc actaatgtct ttagtccgac gctggtacgt cgtcattcag tgtcaactcc
     9121 gtcgctggag gcaagtcatc atgcaaccag cgactaaccg cagttaaagc aattccagcg
     9181 ccagtaattc ttcgatggtc tggcgacggc gaatcaaccg cgcctgacca ttatcaaaca
     9241 gaacttctgg taacagcgga cggctattgt agttggatga cattgatgcg ccatatgccc
     9301 ctgtatcatg cagtaccaga taatcacccg ccttcacttc cggcaaggcg cgggtttcaa
     9361 catttccccc ttcctgctgg gtaaagacat cgcccgattc acataatggc ccggcgacga
     9421 cggtttccac cgttggcgcg tgttccagag aacgaccatc agctgccagg gcactgatat
     9481 ggtggtaact accgtacatt gccgggcgca tcagatcgtt gaacccgaca tcaaccagca
     9541 caaagtggcg gctacccatt tgtttgacgc tccgcacctg agtaattaac acgccagact
     9601 gcgctaccag aaagcgaccc ggttcaattt ccagtttcac agggtggccc aaatggcgag
     9661 cgatttgctc acgcgcggca ttccacagac cataataatg ttcggtatca accgcctctt
     9721 caccctgttg ataaggaata gaaagcccac cgcccgcaga aatagcctgt aaatcctgac
     9781 cgaattcgat gacctgacgt accatagcac cacacacctg ttccagatgg gcataatcaa
     9841 cgccagaacc aatgtgcatg tgaatgccga ccagctgcaa atgatgacgt tgtatcacgt
     9901 ccagtgcggc gggcagatcg gtgtaccaga taccgtgctt gctgttttcg ccaccggtat
     9961 tggttttttg gctatgtccg tgaccaaacc ccgggttaac gcgcagccat acccgatgcc
    10021 ctggcgaaac ctggcccagt tggtcgagca tatcaacaga acccgcattc accggaattt
    10081 gcaattcact gacgcgttca agcgtcgcct gatcgataac atctgccgta aaaacaatat
    10141 catcggggtg cgtttgcgga ttgtaacccg ccgccaacgc acgctctatt tcgcctaacg
    10201 agacggaatc cactttcacg ccctgctcac gcattaagcg caaaatatga atattggaac
    10261 aggctttctg tgcaaagcgc accacatcaa actgtttcag cgctgcaatc tgccgacgaa
    10321 taatttgcgc atcgtagacc cacaccgggc agccaaattc agcgggcaaa cgcagcagat
    10381 tttcggcggt gagatcggta tcggtgctga acagtgaatg tggcataaca aactccagat
    10441 aagtgctttt ttatgattac gccacatcat aaaaagaata aaaaatatcg atttatgtcg
    10501 agtctatgca aaaatgatat ggattaccgg attgcgagag agcgctaatg gccgccgtta
    10561 acttacgtca tattgaaatt tttcatgcgg taatgaccgc cggaagcctg actgaggcag
    10621 cacacctgct acacacctca cagccaaccg tcagccgcga actggcgcgc tttgagaagg
    10681 tgatcgggct aaaattgttt gagcgcgtac gtggacgatt acatcctacc gtgcaaggac
    10741 tgcgtctgtt tgaagaagtg caacgatcct ggtacggact ggatcgcatt gtcagcgccg
    10801 cagaaagtct gcgcgagttt cgccagggag aactgtctat tgcctgcctg ccggtctttt
    10861 cgcaatcttt tttaccgcag ctcctgcaac cctttctggc acgttatccc gatgtcagct
    10921 taaatatcgt gccccaagaa tcaccgctac ttgaagagtg gctctcggcc cagcgtcatg
    10981 atttaggact cactgagaca ctccatacgc ctgcgggaac agaacgtacc gaattactct
    11041 ctttagatga agtgtgtgtg ttacctccgg gtcatccgct ggcggtaaaa aaggtattaa
    11101 cgccggatga ttttcagggt gagaactaca tcagcctttc ccgtactgac agctatcgcc
    11161 agttgctgga tcagctattt actgaacatc aggttaaacg acgcatgatc gtagaaaccc
    11221 acagcgccgc gtcagtctgc gcaatggtac gggcgggggt aggtgtttcg gtggttaacc
    11281 cgctcaccgc actggattat gcggcaagcg gtttagtggt gcggcggttc agtattgcgg
    11341 ttccgttcac cgtcagcctg atccgccccc tgcaccgccc gtcatcagcg ctggtgcagg
    11401 cgtttagtga gcatttacaa gcggggttac cgaaactggt cacttctctt gacgctattt
    11461 tgtcgtcagc tacgacagca taaaagcgac agcatcctcg gcatggatcg ccgcagtatc
    11521 aaacacaggc aaaacactgc gctcttctgg caccagtaaa ccaatttctg tgcagccaaa
    11581 aatgacgccc tgtgcgccct gttctgcaag gcgagcaatc acttgcgcat aataagcgcg
    11641 tgacgcttcg gtaaattgcc ccagacacag ttcttcaaaa ataatctgat taattttcgc
    11701 ccgttcatcc gcttcaggaa taagacaatt gatggaaaat tgttccgtca gccgcccgcg
    11761 ataaaaatcc tgttccatgg tgtaacgcgt acccagcagc gccacacgag tcattccggc
    11821 cccggtaatt gcacgtccgg tggcatccgc aatgtgtaag aaaggcagag tgcaacgtga
    11881 ctcaatggca tccgccactt tatgcatcgt attggtacat agcacaatac cttctgcgcc
    11941 cgcccgctgt aagccaagcg ccgcctcagc cagaatgtcc ccggttttat cccattcccc
    12001 gcgacgctgg cactcttcta tttcatgaaa atcgacgcta tgtagcagca cttgcgcaga
    12061 gtgaagccca ccaagccgct gtttaatgcc ttcatttatc agacgatagt aaggaatggt
    12121 ggattcccag ctcattcctc ccagcaaacc aattgttttc attcctctct cctgatgtgt
    12181 gttaccgcaa caaggctgaa taaggaacga gacaaacgcc tcaacggcca ggtgcccgat
    12241 ctctattaac gaaaaaaggg ccggatgtac agcacatccg gcccatgaaa tcagacgccg
    12301 atatttctca acttctcgcc tgccatcagt ttgcgttcga tatgttccag cgtgacattt
    12361 ttggtttccg gaatgagcca gaaagtgatg cccacaaacg caatgttcag cgcagtgtag
    12421 agccagaacg taccggcagc gccaatgcta tcaagcagtg tcaggaaggt cgcgccgata
    12481 atcatattcg acacccagtt tgtcgtcgtc gaacaggtaa taccgaaatc gcggcatttc
    12541 agcggctgaa tttcagagca caggatccac accactggcg cggcgctcat cgcataaccg
    12601 gcaatacaca tcatcgtcat gccaacagag agccaggaca agccactgga agccgtaccg
    12661 ttatcaaact gcatcaggca atagcccagc accagagtgc ctaacgccat cacgctgaaa
    12721 ccaattttca gagccggttt acgacctgct ttatctaccg taaagaccgc aatgaaggtc
    12781 gcgaacataa aggtcagtcc cacgaccaga gtcgcaatca tctgttgttc tgtggtcgta
    12841 aagcccgcca ttttgaagat acgcggcgca tagtacatga tgatgttcat accggtaaac
    12901 tgctgcatcg cctgcaacaa cataccgaga aacacagcac gacggacgtt acggttgatc
    12961 ttaaacagtg cccaaccgcc ttgttttaac ttcaggcttt cacgaatttc gttgagttct
    13021 tctcgcgctt tttccgacgt atcgcgcagc atacgcaaca cttcttccgc ctcaatatga
    13081 cgcccctttt ccgccagcca gcgcgggcta tttggcagga agaccaccag aataatcagc
    13141 aggactgctg gtaaagcaag aacccccaac attgcgcgcc agttaccgct ataactgaac
    13201 gctgtatcgg ataaaaacgc cagcacgatg ccgagtgtga ccatcaactg gtacatactg
    13261 atcatcttac cgcgaacgtt ttcacttgcc atttcagaaa gatacagagg agcggtgtaa
    13321 gacgcgatcc cgacagcaat gcccagcacc acacgagcgg cgattaacat ctctacgctg
    13381 gtcgcaaaag cggaccctat agaaccgagt acaaacagga tggcccccgc catcaggctg
    13441 tatttacgcc ccaggcggaa cgacagccaa ccattaaaca gcgcaccgat tgctgcgccg
    13501 agcatcatgc tactaaccac ccattcctgc aaacgactgg tcagcacaaa gtgatcggta
    13561 atgaacggca acgctccggc gattacgccg atatcaagac caaataacaa tcctgcgacc
    13621 gcagcagcta ccgaaacaaa catattcata cgccgcgtat cacgcaaaga acgtggcgtt
    13681 aaagcagatt ccgtattgat agtaaccatt ttttcctgcc agcagagagt aagacatagt
    13741 gaaaaaatac gtgaacaatt cacacaggtg tcaggtcgga aacagcataa atatggatta
    13801 aattgctgcg acatgccgtt atgtgatgga tattccaatt ttaaaattaa gttgaattat
    13861 tgagattatt attaaccaac taattttaaa gaagataaaa ttcataaagt tcattaattg
    13921 ataattgata tggattattt cataaccatg atatggatta tgatgatcta caggtataaa
    13981 aaaccctgcc atgcggcagg gtcataaaag taagaagaat gaattaacgc gccagccaac
    14041 cgccatccac ggcaatggta taaccattca cataatctga agcgctggag gcaaggaaca
    14101 ctaccggccc catcaggtca ctcggcagtc cccaacggcc agctggaatg cggtcgagaa
    14161 tttccgcgct acgttgttca tctgcccgca gttgttgagt attgttggtc gccatgtaac
    14221 ccggagctat cgcattaaca ttaatgttgt gtttagccca ttcgttcgcc atcaatcgcg
    14281 tcacgcccat cacaccgctt tttgatgcgg tataagaagg cacacggatc ccgccctgga
    14341 aggagagcat tgacgcgata ttgataatct tgccgccatt gccttgcgcg ataaagtgtt
    14401 tcgccgctgc ctgagacatg aagaatacgc tcttgatatt caggttcatg acatc
//
LOCUS       DAMZET010000107        14454 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.107, whole genome shotgun sequence.
ACCESSION   DAMZET010000107 DAMZET010000000
VERSION     DAMZET010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.107"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1287
                     /locus_tag="POB34_003224"
     CDS             <1..1287
                     /locus_tag="POB34_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDI5920116.1"
                     /translation="RLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLIIAVVVALSFGRDAHGWTWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(1322..2602)
                     /gene="hemL"
                     /locus_tag="POB34_003225"
     CDS             complement(1322..2602)
                     /gene="hemL"
                     /locus_tag="POB34_003225"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDI5920117.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTSRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDGGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            2827..4248
                     /gene="clcA"
                     /locus_tag="POB34_003226"
     CDS             2827..4248
                     /gene="clcA"
                     /locus_tag="POB34_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HDI5920118.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            4272..4337
                     /locus_tag="POB34_003227"
     CDS             4272..4337
                     /locus_tag="POB34_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920119.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            4330..4674
                     /gene="erpA"
                     /locus_tag="POB34_003228"
     CDS             4330..4674
                     /gene="erpA"
                     /locus_tag="POB34_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HDI5920120.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(4721..5344)
                     /locus_tag="POB34_003229"
     CDS             complement(4721..5344)
                     /locus_tag="POB34_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HDI5920121.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(5382..6182)
                     /gene="btuF"
                     /locus_tag="POB34_003230"
     CDS             complement(5382..6182)
                     /gene="btuF"
                     /locus_tag="POB34_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HDI5920122.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(6175..6873)
                     /gene="mtnN"
                     /locus_tag="POB34_003231"
     CDS             complement(6175..6873)
                     /gene="mtnN"
                     /locus_tag="POB34_003231"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDI5920123.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIEKRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            6957..8474
                     /gene="dgt"
                     /locus_tag="POB34_003232"
     CDS             6957..8474
                     /gene="dgt"
                     /locus_tag="POB34_003232"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HDI5920124.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCYFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            8604..10028
                     /gene="degP"
                     /locus_tag="POB34_003233"
     CDS             8604..10028
                     /gene="degP"
                     /locus_tag="POB34_003233"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HDI5920125.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            10183..11340
                     /gene="cdaR"
                     /locus_tag="POB34_003234"
     CDS             10183..11340
                     /gene="cdaR"
                     /locus_tag="POB34_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HDI5920126.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(11429..11815)
                     /locus_tag="POB34_003235"
     CDS             complement(11429..11815)
                     /locus_tag="POB34_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HDI5920127.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(12130..12954)
                     /gene="dapD"
                     /locus_tag="POB34_003236"
     CDS             complement(12130..12954)
                     /gene="dapD"
                     /locus_tag="POB34_003236"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HDI5920128.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(12985..>14454)
                     /gene="glnD"
                     /locus_tag="POB34_003237"
     CDS             complement(12985..>14454)
                     /gene="glnD"
                     /locus_tag="POB34_003237"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HDI5920129.1"
                     /translation="HARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLG
                     AYMPQWSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPS
                     TELIFIAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSV
                     TAQRRDIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYF
                     ATEKQLRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSP
                     NQLAWHARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSV
                     HDAQIFTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPA
                     KLRHFTVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIG
                     ERVEDLFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
BASE COUNT         3345 a         3632 c         3939 g         3538 t
ORIGIN      
        1 cgtctggcag cgctgtcgct ggcgattgtc atcagtgcgc tgctggtgaa cgctgtgggg
       61 attatcggct ttatcgggtt gttcgcgccg ctgctggcaa aaatgctggg ggcgcggcgt
      121 ctgctgccac gactgatgct ggcgtcgctg attggggcgc tgatcctctg gctttccgat
      181 caaatcatcc tctggctgac tcgcgtgtgg atggaagtgt ccaccggttc ggtcactgcg
      241 ttgatcggtg cgccgctgct actgtggctg ttgccgcgtt tacgcagcat tagcgcgccg
      301 gatatgaagg tcaacgatcg tgtcgcggct gaacgccaac atgtgctggc gtttgccctt
      361 gctggcggtg tgctgctgat aattgctgtg gtggtggcgc tgtcgtttgg tcgtgatgcg
      421 cacggctgga cgtgggcgag cggggcgctg ctcgatgatt taatgccctg gcgctggccg
      481 cgaattatgg cggcgctgtt tgcgggcgtc atgctggcgg tggcgggctg tattattcag
      541 cggctgaccg gaaacccgat ggcaagcccg gaagtgctgg ggattagctc tggcgcagcg
      601 tttggcgtgg tgttgatgct gtttctggtg ccgggtaatg cctttggctg gttgttacct
      661 gcaggcagtc tcggggcggc ggtgacgctg ttgatcatta tgatcgccgc cggacgcggt
      721 ggattttccc cacaccgcat gttactggcg gggatggcgt taagtaccgc gttcaccatg
      781 cttttgatga tgttgcaggc aagtggtgac ccgcgaatgg cgcaagtgct gacctggatt
      841 tccggttcga cctacaacgc gaccgatgcg caggtctggc gcaccggaat tgtgatggtg
      901 attttgctgg cgattacccc gctgtgccgc cgctggctga ccattttacc gctgggtggt
      961 gataccgccc gagccgtagg aatggcgcta acgccgacgc gaattgcgct gctgctgtta
     1021 gcggcttgcc tgacggcgac cgcgacgatg actattggac cgttgagttt tgttggttta
     1081 atggcaccgc atattgcgcg gatgatgggc tttcgacgga cgatgccgca catcgtaatt
     1141 tcggcgctgg tgggtggttt actgctggtg ttcgctgact ggtgtgggcg gatggtgctg
     1201 tttccattcc agatcccggc ggggctgctg tcaaccttta tcggcgcgcc atattttatc
     1261 tatttgttga gaaagcagag ccgttaattg aatgcctcgc ccggtgggcg aggcaaaatc
     1321 atcacaactt cgcaaacacc cgacgtgcag catcgatggt gttattgata tcttccatgc
     1381 tgtgcgccac ggacataaag cccgcttcaa acgctgacgg tgccaggtaa acacctccat
     1441 ccagcatcat atggaagaaa cgcttaaagc gttccacgtc acaggccatc acatcctgat
     1501 agcacgtcac ggactcggcg tcggtaaaga aaataccgaa catgccgcca acgtggttaa
     1561 cgaccagcgg aattccggct tcttctgccg cttccagcag accttctgcc agacgtgatg
     1621 tcagctcatc caacgtttcg tgaacgcccg gctgcgcgac ttcattcaga caggcgaaac
     1681 cggctgccat cgcaattggg ttaccggaaa gcgtacccgc ctgatagacc ggacccgtcg
     1741 gggccagcgc atccattaca tcacgacgac caccgaatgc gcctaccggc attccaccgc
     1801 cgatgatttt tccgaggcag gttaaatctg gcactacgcc gtaataatcc tgtgcaccag
     1861 ccagtgccac gcggaagcca gtcattactt catcgatgat cagcaatgcg ccaaattcgt
     1921 cgcacagcgc gcgcagacct ggcaggaact ctggcagcgg cggaacacag ttcatattgc
     1981 ctgccaccgg ctcaacgata atacaggcaa tctcttgcgg gtattgctca aacgcggcgc
     2041 gtacagaagc cagatcgtta taagtacagg ttaaggtatg tttggcgaaa tctgccggaa
     2101 cacccggcga gtttggctgg cctaacgtga gtgcgccaga accggctttc accagcaggc
     2161 agtcagcgtg accgtggtaa caaccttcaa atttaatgat tttgtcgcga ccggtaaaac
     2221 cacgggccag gcggatggcg ctcatggtcg cttcagtgcc ggagttcacc atgcgcacca
     2281 tatccatggt cgggaccagt tcggtcacca gttgcgccat tttcacttcc atttcggttg
     2341 gtgcaccaaa gcttaaacca cgctcggcgg cttcaatcac ggcattgcgg attgccggat
     2401 ggttatggcc cagcaccatc ggcccccagg aaccgacata atcgatatag gctttgccat
     2461 caacatcgta cagataagcg ccgtccgctt tttcgataaa cagtggagtg ccgcccacgc
     2521 cagtaaaggc gcgaacaggg gagttcacac cgccagggat cagctcgcgc gctgcgctgt
     2581 aaagattttc agacttactc atggagggtt cctgattcgt agaaaaagtg aatggctgct
     2641 attctatgtt attcataaca agttaaatac tcgtcaaaca tcaggctgct tgtactggtg
     2701 caatcctgat ttcttagagt ataaaagttt tgtgcatttg aaacattacg ctttgcaaag
     2761 gattttcatg gaacgtgcga gtaaaatgcc gtcatcttat ttgtatgacc aataagtgat
     2821 cattggatga aaactgatac tccctcttta gaaacaccgc aggccgcgcg cctgcgacgc
     2881 agacaactga ttcgccaact tcttgagcgc gataaaaccc cgttagccat tttgtttatg
     2941 gcggcagtcg tcggcacgct tgtcgggctg gctgcggttg cttttgacaa aggtgtcgcc
     3001 tggttgcaga accaacgtat gggggcgctg gtacatactg ctgataatta tccgctactg
     3061 ttaactgtcg cttttctctg ttcggcggtg ctggcgatgt tcggctactt tttggtgcgc
     3121 aaatacgcgc ccgaagcagg gggatcgggg atcccggaaa ttgaaggggc gctggaagat
     3181 caacgtcctg ttcgctggtg gcgtgtattg ccggtgaaat tctttggcgg gctggggaca
     3241 ctcggcggag gcatggtgct agggcgcgaa ggaccaaccg tgcagatcgg cggcaatatc
     3301 ggtcgcatgg tgcttgatat tttccgcctg aaaggtgatg aagctcgcca tacgctgctg
     3361 gcaaccggtg ctgctgcggg gctggctgcg gcctttaacg cgccgctggc gggtattttg
     3421 tttattatcg aagagatgcg tccgcagttt cgctatacgt taatttcgat taaagcggta
     3481 tttattggcg tcattatgtc gaccattatg taccggattt ttaatcatga agttgcgttg
     3541 attgacgtcg gtaaactttc tgatgcgccg cttaatacgc tgtggcttta tctgatcctc
     3601 ggtattattt ttggcatttt cggccctatt tttaataaat gggtgctggg gatgcaggat
     3661 ttgctgcacc gtgtgcacgg cggcaatatt accaaatggg tgctaatggg cggtgcgatt
     3721 ggcggtctgt gtggattgct ggggtttgtg gcaccagcaa cgtcgggcgg cggttttaac
     3781 ctgattccta tcgctaccgc ggggaatttc agcatgggaa tgctggtgtt tatcttcgtc
     3841 gcgcgggtca ttaccacctt actctgcttc tcttccggcg cgccgggcgg tatttttgcc
     3901 ccgatgctgg cgctgggtac tgtgctggga accgctttcg gaatggttgc cgttgagctg
     3961 tttccgcaat atcaccttga ggcgggaacg tttgctattg ccggaatggg ggcgttactg
     4021 gcggcatcta ttcgcgcgcc gttaacgggg atcattctgg ttctggagat gaccgataac
     4081 taccagctca ttttgccaat gattattacc ggtcttggcg caacactatt agcgcaattt
     4141 accggcggga aaccgctata ctcggcgatt cttgcgcgca cgctggcaaa acaggaagct
     4201 gagcaactgg cgcgaagcaa ggccgcatca gccagcgaga atacttgaac gaaataccag
     4261 ggtattagat aatggcgatt attattgggt tagaatttgc ccaattgccg atgtcgtttg
     4321 gagcaaaata tgagtgatga cgtagcactg ccgctggagt ttaccgacgc agcagccaac
     4381 aaagttaaaa gcctgatcgc tgacgaagat aacccgaatc tgaaattacg cgtgtatatc
     4441 accggtggcg gttgcagcgg cttccagtat ggtttcacct ttgatgatca ggtgaacgaa
     4501 ggcgatatga ccatcgaaaa acagggcgtt ggcctggtgg ttgatccgat gagcctgcaa
     4561 tatctggtcg gcggttccgt tgactatacc gaaggactgg aaggttctcg tttcatcgtg
     4621 accaacccga acgcgaaaag cacctgcggt tgcggttctt cctttagcat ctaatctgtt
     4681 gttcatgtgc cggataaact atccggcaca tcttctctct tcaacgccca ttctcatcca
     4741 gcgcaaacgt cggtagctta agatgccaac gaatagccgc cagccgaatc aatagcgtca
     4801 cgaccatgcc catcatactg gctgtttcca gtggtacgga aaatgtgtaa taagccgtag
     4861 cgtggacaat accgccgata atacaggcag ttgcgtagat ttctgtacgt aaaatcatgg
     4921 ggatctcgcg ggccagtaca tcacgaataa tcccgccgcc aacgccagta atgacgccca
     4981 tacaaaccgc gattaacgga ccggcttccg cattaaaggc tttattcacg ccaatgccga
     5041 caaacaccgc cagaccaacg gcgtccagca ccggcaacat ccattttggt aagcgtcttg
     5101 gctggcgcac cagcacgatg gtcagcatgc tggtgaccat tgcaacgacc agatcggtgg
     5161 gatctttcac ccaaaatacc gggccgtgat ccagcgccat gtcgcgaatt gttccgccgc
     5221 ctactgcggt taccacgccc agtaccagaa caccaaaagg gtccatacgc aattttccgg
     5281 ctaacaaaac gccggagatg gcaaataccg ctgtgccgac tatatccagc caatagacga
     5341 gcattgttca atccccactg agcacctgaa aaggtcaggc actaatctac ctgtgaaagc
     5401 gcattacaga gctgttgtgc tgcgaggata atacgtgggc ttgcacgttc aaaccagtca
     5461 ctcgtgagag gaataacggg aattttgagc tgttcacccc agtattgttt gattttagga
     5521 atttggtccg gtccgcctgt aatgacaatc gcctgtggcg agcgtgctaa cacctgttcg
     5581 cggctaactt gcggccaggg aacccggctg tctttaaaga tgttttctcc gccacaaact
     5641 tcgagtacct ggttctgaat cgactctttt ccactggtaa atggcggatt aatgccgaat
     5701 tgcagaaaaa cacgtttttt aggtttatca gcatattgcg ctttcaattg cgcgtactga
     5761 tccagcaggg attgcgcggc ttgttcggcc ttgtctggtt gcggactcca gggggccagt
     5821 tgacgtaacg cattggcaat ttgttcaatg cttgtcgcat cgacccacat cacttttatt
     5881 cccagcgaag ccagctggtc aacctgccgc tcggcattac ctccacgcca ggcaatcacc
     5941 agatcgggct tcagcgcgac aatgcgttcc agattcatcc cctgccaggt ggaaacctgc
     6001 tcaatctttt gcgcttgtgg aggatagtcg gaatagctgc tgaccccaac cggcgtgatc
     6061 ccggcagcaa aggcaagttc agtgttggcg ggagaaagcg tgatgacgcg cggcgcggcg
     6121 ttgagccaca gtggcgcaag aaaagacagg gcgaccagcg ccctgaacag tgacttagcc
     6181 atgtgcaagt ttctgcacca gtgactcaac catcaggctg gactgtttag cggcaacagc
     6241 caggaactca tcgaagctaa gatgagactg ttgatcggcc acgtcggaga tggcgcgtac
     6301 gacaacaaac gggacgttga aattgtggca gacatgggcg attgccgtcg cttccatctc
     6361 tacagcaatg gcctgtggga agttgtggcg gattttcgcc agaccaacag aaccgttgat
     6421 gaaagcgtcg ccgctaacaa tcaggccacg tacagcgtta agattcagtt cggcaatgca
     6481 ggcctcagcg gcagcgatca gtttatcgtc agctttaaag cctgccggac agcctggtaa
     6541 ctgaccgtat tcataaccaa atgccgtgac atccgcgtcg tgataacgtg cttcgtccga
     6601 gacaacgata tcgcccactt tcaacgttgg tgccaggcca ccggcagaac cggtgttaat
     6661 aatcacatct ggcttgcagt gttccaacag caaagtggca cccagcgccg cagcgacttt
     6721 accgatgccc gatttcagaa gcgcaacctc ggttccattc agttggccgg tatagatttc
     6781 gcaaccgccg agactgatag tttgacgttt ttcgattttg tcacgcagca gcgtaacttc
     6841 ttcttccatt gcaccaatga tgccgatttt catagattta ctcgcgataa gcccgatttg
     6901 aaggcatagt ttaccatgcg cttacgggga agcgtatttc tcacgcggga gaggacatgg
     6961 cacagattga tttccgaaaa aaaataaact ggcatcgtcg ttaccgttca ccgcagggcg
     7021 ttaaaaccga acatgagatc ctgcggatct tcgagagcga tcgcgggcgt atcatcaact
     7081 ctccggcaat tcgtcgtctg caacaaaaga cccaggtttt tccactggag cgcaatgccg
     7141 ccgtgcgcac gcgtcttacc cactcgatgg aagtccagca ggtggggcgc tacatcgcca
     7201 aagaaattct aagccgtctg aaagagctta aattactgga agcatacggc ctggatgaac
     7261 tgaccggccc ctttgaaagc attgttgaga tgtcatgcct gatgcacgat atcggcaatc
     7321 cgccgtttgg tcattttggc gaagcggcga taaatgactg gtttcgccag cgtttatacc
     7381 cggaagatgc cgaaagccag cctctgactg acgatcgctg cagcgtggca gcactacgtt
     7441 tacgggacgg ggaagaaccg cttaacgagc tgcggcgcaa gattcgtcag gacttatgtt
     7501 attttgaggg gaatgcacaa ggcattcgtc tggtgcatac attgatgcgg atgaatctca
     7561 cctgggcaca ggttggcggt attttaaaat atacccgtcc ggcgtggtgg cgtggcgaaa
     7621 cgcctgagac acatcactat ttaatgaaaa agccgggtta ttatctttct gaagaagcct
     7681 atattgcccg gttgcgtaaa gaacttaatt tggcgcttta cagtcgtttt ccattaacgt
     7741 ggattatgga agctgccgac gatatctcct attgtgtggc agaccttgaa gatgcagtag
     7801 agaaaagaat atttaccgtt gagcagcttt atcatcattt gcacgaagcg tggggccagc
     7861 atgagaaagg ttcgctcttt tcgctggtgg ttgaaaatgc ctgggaaaaa tcacgctcaa
     7921 atagtttaag ccgcagtacg gaagatcagt tttttatgta tttacgggta aacaccctaa
     7981 ataaactggt accctacgcg gcacaacgat ttattgataa tctgcctgcg attttcgccg
     8041 gaacgtttaa tcatgcatta ttggaagatg ccagcgaatg cagcgatctt cttaagctat
     8101 ataaaaatgt cgctgtaaaa catgtgttta gccatccaga tgtcgagcag cttgaattgc
     8161 agggctatcg ggtcattagc ggattattag agatttatcg tcctttatta agcctgtcgt
     8221 tatcagactt tactgaactg gtagaaaaag aacgggtgaa acgtttccct attgaatcgc
     8281 gcttattcca caaactctcg acgcgccatc ggctggccta tgtcgaggct gtcagtaaat
     8341 taccgtcaga ttctcctgag tttccgctat gggaatatta ttaccgttgc cgcctgttgc
     8401 aggattatat cagcggtatg accgacctct atgcgtggga tgaataccga cgtctgatgg
     8461 ccgtagaaca ataaccaggc ttttgtaaag acgaacaata aatttttacc ttttgcagaa
     8521 actttagttc ggaacttcgc gctataaaac gaatctgaag aacacagcaa ttttgcgtta
     8581 tctgttaatc gagactgaaa tacatgaaaa aaaccacatt agcactgagt gcactggctc
     8641 tgagtttagg tttggcgtta tctccgctct ctgcaacggc ggctgagact tcttcagcaa
     8701 cgacagccca gcagatgcca agccttgcac cgatgctcga aaaggtgatg ccttcagtgg
     8761 tcagcattaa cgtagaaggt agcacaaccg ttaatacgcc gcgtatgccg cgtaatttcc
     8821 agcagttctt cggtgatgat tctccgttct gccaggaagg ttcaccgttc cagagctctc
     8881 cgttctgcca ggggggccag ggcggtaatg gcggcggcca gcaacagaaa ttcatggcgc
     8941 tgggttccgg cgtcatcatt gatgccgata aaggctatgt cgtcaccaac aaccacgttg
     9001 ttgataacgc gacggtgatt aaagtccagc tgagcgatgg tcgtaagttc gacgcgaaga
     9061 tggttggcaa agatccgcgc tctgatatcg cgctgattca gatccagaac ccgaaaaacc
     9121 tgaccgcaat taagatggcg gattctgatg cactgcgcgt gggtgattac accgtagcga
     9181 ttggtaaccc gtttggcctg ggagagacgg taacttccgg gattgtctct gcgctggggc
     9241 gtagcggcct gaatgccgaa aactacgaaa acttcatcca gaccgatgca gcgatcaacc
     9301 gtggtaactc cggtggtgcg ctggttaacc tgaacggcga actgatcggt atcaacaccg
     9361 cgatcctcgc accggacggc ggcaacatcg gtatcggttt tgctatcccg agcaacatgg
     9421 tgaaaaacct gacctcgcag atggtggaat acggccaggt gaaacgcggt gagctgggta
     9481 ttatggggac tgagctgaat tccgaactgg cgaaagcgat gaaagttgac gcccagcgcg
     9541 gtgctttcgt aagccaggtt ctgcctaatt cctccgctgc aaaagcgggc attaaagcgg
     9601 gtgatgtgat cacctcactg aacggtaagc caatcagcag ctttgccgca ctgcgtgctc
     9661 aggtgggtac tatgccggtg ggcagcaaac tgaccctggg cttgctgcgc gacggtaagc
     9721 aggtcaacgt gaatctggaa ctgcagcaga gcagccagaa tcaggttgat tccagctcca
     9781 tcttcaacgg cattgaaggt gctgagatga gcaacaaagg caaagatcag ggcgtggtag
     9841 tgaacaacgt gaaaacgggc actccggctg cgcagatcgg cctgaagaaa ggtgatgtga
     9901 ttattggcgc gaaccagcag gcagtgaaaa acatcgctga actgcgtaaa gttctcgaca
     9961 gcaaaccgtc tgtgctggca ctgaacattc agcgcggcga cagctccatc tacctgttaa
    10021 tgcagtaatc tccctcaacc ccttcctgaa aacgggaagg ggttctcctt acaatctgtg
    10081 aacttcacca caactccata catcttcatc atcctttagg catttgcaca atgccgtacg
    10141 ttacgtactt ccttatgcta agccgtgcat aacggaggac ttatggctgg ctggcatctt
    10201 gataccaaaa tggcgcagga tatcgtggca cgtaccatgc gcatcatcga taccaatatc
    10261 aacgtaatgg atgcccgtgg gcgaattatc ggcagcggcg atcgtgagcg tattggtgaa
    10321 ttgcacgaag gtgcattgct ggtactttca cagggacgag tcgtcgatat cgatgacgcg
    10381 gtggcacgtc atctgcacgg tgtgcggcaa gggattaatc taccgttacg gctggaaggt
    10441 gaaattgtcg gcgtaattgg cctgacaggt gaaccagaga atctgcgtaa atatggcgaa
    10501 ctggtctgca tgacggctga aatgatgctg gaacagtcgc ggttgatgca cttgttggct
    10561 caggatagcc gtttgcggga ggaactggtg atgaacctga ttcaggcaga ggagaatact
    10621 cccgcactta ctgaatgggc gcaacggctg gggatcgatc tcaatcaacc gcgagtggtg
    10681 gctattgttg aggtcgacag cggtcagctt ggtgtggaca gcgcaatggc ggagttacaa
    10741 caactgcaaa acgcgctgac tacgcccgag cgtaataatc tggtggcgat tgtctcgcta
    10801 accgaaatgg tggtgttgaa accggcgttg aactcttttg ggcgctggga tgcagaagat
    10861 catcgtaagc gagttgaaca actgattacc cgcatgaaag agtacggcca gctgcgtttt
    10921 cgcgtttcac tgggcaacta ttttaccggt cctggcagta ttgcccgatc ctatcgtacg
    10981 gcgaaaacga cgatggtggt gggtaaacag cggatgccag aaagtcgctg ctatttttat
    11041 caggatctga tgttacctgt gttactcgac agtttgcgtg gcgactggca ggccaacgaa
    11101 ctggcgcgac cgctggcgcg gctgaaagcg atggacaata acggcttgct gcgacgaacg
    11161 ctggcggcgt ggtttcgtca caatgtgcaa ccgctggcaa cgtcaaaggc gttgtttatt
    11221 catcgtaata ctctggagta tcggcttaat cgtatatcgg aactgaccgg gcttgatttg
    11281 ggtaattttg atgacaggtt gctgctgtat gtggcgttgc agctggatga agagcggtag
    11341 gttatgcgtt aaggtcgagg cggcgctggc tcattcccct cagcccgaag ggtagagggg
    11401 aatgtccgag ttaaccgtca gttcggcatt atttacgcgt tagtttttcc agatcggctt
    11461 caatctcgct gatcttattg gttacgactg actccaggtg acgtaagtcg tcgaggatct
    11521 tacgcttaag atcaacttcg ctgcggtcac gctggcagat ttgatcaagc tcatcaatga
    11581 tataccgtag attcgggctg atttcctgga cttctttata accctgaccc acaccatcag
    11641 cgacgaccgt tttacgctga cgcggatatt taaacttaac gctcttggcg aaaaactcgc
    11701 ctttgtcttt ctggaaatag attttcagaa tatcgttgtt ggcttcctgc cggaggctgt
    11761 aacgatcaat ttcttcagga ttggtaatac ccagactttt cagattgtcg tacatagcgt
    11821 tacctcaaaa tgagtcagta aattgtgctt attttagcat ttggcctggc ccgcccggct
    11881 cgactgtgat cgcagaaagc ctttttcttc cagacttcta tcatggcgta aaaatcaaaa
    11941 aattacctgc tttattgtgg cgatattcca tgggatggat taacacattt atcttcgttt
    12001 tgcgaattac atgacagtcc gaaacaagat gaaaaagtat ttaaaaaagg ggggaagaag
    12061 aatgagtagg acgaattatt ctgagccaga aagcatacgg gcagcatcat gctgcccgta
    12121 tatgtactct taatcgatgg tacgcagcag ttcgttaatg ccgactttgc cgcgagtttt
    12181 cgcgtcaact ttcttaacga taaccgcaca gtagaggctg tatttgccat ctttcgacgg
    12241 cagattacct gaaacaacca cagaccccgc cggaacgcga ccgtagtgga tttcgccggt
    12301 ttcacggtcg taaatacggg tgctctgacc aatgtatacg cccatagaga tgacggaacc
    12361 ttcttcgaca atcacccctt caaccacttc ggagcgtgcg ccaatgaagc agttatcttc
    12421 aataatggtc gggttagcct gcagcggctc cagcacgccg ccgatgccca cgccaccgga
    12481 aaggtggacg tttttaccaa tctgcgcaca agaaccgacg gtcgcccagg tatcaaccat
    12541 cgtgccttca tcaacatatg cgccgatgtt gacgtaagac ggcatcagca cggtgttacg
    12601 ggcaataaat gcaccctgac gtaccgccgc tggtggcaca acgcggaagc cttctttctg
    12661 aaaacgcgct tcgtcgtagt cggcgaattt catcggcact ttatcgaagt agcggctttc
    12721 tgccccttcg atcacctgat tatcattaat acggaaagag agcagcaccg cttttttcaa
    12781 ccactgatgc gtcacccact gaccgtcaat tttttccgct acacgcagtg cgccggaatc
    12841 cagcagggcg atcacctgat ttaccgcttc gcgggttacg gtgtctgcat tggctggcgt
    12901 gatctcggca cggcgttcaa aagcggtttc aataatgttc tgtaactgct gcattgttaa
    12961 actcttttca tatcagtaaa cacatcaccc tttatcgttt ggattgaggg cctctgtcaa
    13021 ccgctgatgc acttcctgct gcaactcgtt attaagtgca cgccggtcag cggtggcaat
    13081 tatgaataaa tcttctactc gctcgccaat ggttgtaatt cgggcaccat gaagcgaaat
    13141 tcccagatcg gcaaaaattt tcccgactct cgccagcagt cctggctggt cgagcgcgat
    13201 aagctcaagg aacgatttgc ggtcggtatg ggtcggcaaa aacgttactt cggtttcaac
    13261 agtaaaatgg cgtaatttgg cgggttggcg acggggctgc ggtggctgcc agctactttg
    13321 cgtcagtact tgctccagac caaaccgaat aacctcatga cgatctgcgg acagcgggct
    13381 gccatcgggt tccagcacga taaaggtatc catcgccata ccgtcgcgag tggtgaaaat
    13441 ttgtgcgtcg tgaacactta aattgcggcg gtctaattcg gcacagacgg cggcaaacag
    13501 ataggggcgg tccgggctcc agataaaaat ctcggtgcct ccacgcgtag cctgcgggct
    13561 aagcaatacc agcggtttgc ttaaatcatg ctgtaataaa tggcgggcat gccaggccag
    13621 ttgatttggg ctatggcgga caaaatagtt agcacgacag cgtgaccaaa tttggtgcag
    13681 cgcctcttcg tcgatgttat ccatgcgcag tagtgccagt gcctggagtt ggtgatggcg
    13741 aacccgttcg cgcatatccg gcgtgttttg cattccgcgt cgtagctgct tttcggtggc
    13801 aaaatagagt tcgcgcaaca gactttgctt ccagctattc cacagcgttt cgttggtggc
    13861 gcaaatgtca gccactgtca ggcataccag atagcgcaga cgattttccg tttgaacttc
    13921 ttcggcaaac tgcttgatga cttcagggtc ctgaatatcg cggcgttgtg ctgtcaccga
    13981 catcagcaag tgctggcgaa ccagccaggc aaccagctgt gtttcgcgtg agttcagccc
    14041 gtggagttct gcaaaatgca ctacatcctg agcaccgaga atggagtggt cgccgccgcg
    14101 tcctttggcg atatcgtgaa acagcgcggc gatgaaaatc agctcagttg acggcaggcg
    14161 cggccagacg tccacacaca acggatggcg ctggcgcgtt tcttcactgg caaaactctc
    14221 cagtttcagc atcacgcgga tagtatgttc atccaccgtg taggcgtgga acagatcaaa
    14281 ctgcatctgc ccgacgatat gcgaccattg cggcatatac gcgccgagca cgctatggcg
    14341 atgcattggc aatagcccgc gccgcaccgc tccggggtga cgcagaatgc tcaaaaacag
    14401 ttttcgtgct tccggaatat tacacagcgg ttgttgcaga tggcgacggg catg
//
LOCUS       DAMZET010000108        14192 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.108, whole genome shotgun sequence.
ACCESSION   DAMZET010000108 DAMZET010000000
VERSION     DAMZET010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.108"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..925)
                     /locus_tag="POB34_003238"
     CDS             complement(<1..925)
                     /locus_tag="POB34_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="necessary for biosynthesis of osmoregulated
                     periplasmic glucans possibly involved in the transfer to
                     the periplasmic space; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis glucosyltransferase H"
                     /protein_id="HDI5920130.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGG"
     gene            complement(918..2453)
                     /gene="mdoG"
                     /locus_tag="POB34_003239"
     CDS             complement(918..2453)
                     /gene="mdoG"
                     /locus_tag="POB34_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HDI5920131.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            2847..4004
                     /gene="mdoC"
                     /locus_tag="POB34_003240"
     CDS             2847..4004
                     /gene="mdoC"
                     /locus_tag="POB34_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HDI5920132.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(4012..5493)
                     /gene="clsC"
                     /locus_tag="POB34_003241"
     CDS             complement(4012..5493)
                     /gene="clsC"
                     /locus_tag="POB34_003241"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HDI5920133.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(5435..5968)
                     /gene="ymdB"
                     /locus_tag="POB34_003242"
     CDS             complement(5435..5968)
                     /gene="ymdB"
                     /locus_tag="POB34_003242"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HDI5920134.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(6063..6374)
                     /locus_tag="POB34_003243"
     CDS             complement(6063..6374)
                     /locus_tag="POB34_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDI5920135.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(6495..6827)
                     /gene="csgC"
                     /locus_tag="POB34_003244"
     CDS             complement(6495..6827)
                     /gene="csgC"
                     /locus_tag="POB34_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HDI5920136.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(6886..7341)
                     /gene="csgA"
                     /locus_tag="POB34_003245"
     CDS             complement(6886..7341)
                     /gene="csgA"
                     /locus_tag="POB34_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HDI5920137.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(7382..7837)
                     /gene="csgB"
                     /locus_tag="POB34_003246"
     CDS             complement(7382..7837)
                     /gene="csgB"
                     /locus_tag="POB34_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HDI5920138.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            8591..9241
                     /gene="csgD"
                     /locus_tag="POB34_003247"
     CDS             8591..9241
                     /gene="csgD"
                     /locus_tag="POB34_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HDI5920139.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            9246..9635
                     /gene="csgE"
                     /locus_tag="POB34_003248"
     CDS             9246..9635
                     /gene="csgE"
                     /locus_tag="POB34_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HDI5920140.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            9660..10076
                     /gene="csgF"
                     /locus_tag="POB34_003249"
     CDS             9660..10076
                     /gene="csgF"
                     /locus_tag="POB34_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HDI5920141.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            10103..10936
                     /gene="csgG"
                     /locus_tag="POB34_003250"
     CDS             10103..10936
                     /gene="csgG"
                     /locus_tag="POB34_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HDI5920142.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(11000..11491)
                     /locus_tag="POB34_003251"
     CDS             complement(11000..11491)
                     /locus_tag="POB34_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDI5920143.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(11593..12147)
                     /gene="ycdY"
                     /locus_tag="POB34_003252"
     CDS             complement(11593..12147)
                     /gene="ycdY"
                     /locus_tag="POB34_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HDI5920144.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(12171..12908)
                     /gene="ycdX"
                     /locus_tag="POB34_003253"
     CDS             complement(12171..12908)
                     /gene="ycdX"
                     /locus_tag="POB34_003253"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HDI5920145.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(12963..13901)
                     /gene="ghrA"
                     /locus_tag="POB34_003254"
     CDS             complement(12963..13901)
                     /gene="ghrA"
                     /locus_tag="POB34_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HDI5920146.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGACLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGEKVCGQVDRARGY"
BASE COUNT         3695 a         3503 c         3211 g         3783 t
ORIGIN      
        1 cgccaccgac ttcagcgata agctccatcc aggctttttg ctctgcgacg cagatatccg
       61 ggttataact gtcactaaga atgtagacat caaagtgttt ggcattcccg gtggctttta
      121 ctgattccca cgttgcacgc aggccagcaa aaacacggtt cacgtcttcg ttacagatag
      181 gcatgatcaa cgccgtgcga tgctccgggt ttaatggttc atcgccaact gttgacgcag
      241 atatactgta tttatcgcga ccaataagca gttgcaggaa gcccattaac gccgtccaga
      301 atccggcgga cacccaacag aacagtaccg caaagaggat caggataccg gtttgcagca
      361 tataaggcag aagctgcata aaggaaaccc acaaatcctg accaaccata tccataggat
      421 taatcagcgc ccaaccctga taaggaagaa tggtcttcat ataccaggtc gcgacgacag
      481 tttgcgcgag cgtcaggatc aacagaatgt aacggcggat ggtaccgacg gtacgccact
      541 tttgctcgct ctcctgctct tctttggtca aacgagccag atagcgcggc gtgacatcgc
      601 gtccacgcag gcgatcccag aaacggccta ccgggttggt acgccacggg tcgggaaaca
      661 tcgaggagcg ttttgcttct ggcatcgcct tcagctgatc gcgcccttcg tcgtctttaa
      721 ttaactgtcc atcagcaagt gaatctggcc aggcttgttc cagacgcgcc tttaccgagc
      781 cttgcgggga atcatcctcc cgcgcccagg tgcggtgttc ggcatccagc gcctgatgaa
      841 cggcgcggat atcagtcttc ggcaatgccg ctttctcgct tgcggcgatg ggcattgcgt
      901 caatgtactc agttgtctta ttcattggca ggtaactggt agctccaggt ttcactcaac
      961 gtctgatcgg cattcaccag cgcagcacgc atttcagtgg ttttcttggc atctttcact
     1021 ttcacacgca tcaccagacg ccagcctttg gtaaccgggt tatagcgcac cgtgctttca
     1081 actatctcac cattatcacc aatgctggtt tgcgctgtga ccggggtatc ctctggcagt
     1141 tttttcatct ctgcgccggt aaaatcgacc acaaaggcga tagtaccgtc aggctggcga
     1201 atcaggttcg actgcttcac atcccccgtt gaacgacgcg tttgttgcac ccatgcgtta
     1261 tctggcgcat gcagtttgtc ttcatcacgg ctgaaggtga tggtgtattt aaagttcatc
     1321 tctttacccg gctccggcag ctgatccggc gtccagtaag cgacgatgtt atcgttggtt
     1381 tcatcgttgg ttggaatttc caccagctca acgctgcctt taccccactc ccctttcgga
     1441 gtcacccatg cgcttggacg aagatcgtaa cgatcatcga gatcttcaaa gcgggagaaa
     1501 tcacgaccgc gctgcaacag accgaagcct tgcgggttct ccatcgagaa gctgctgacc
     1561 gctaaatgtt tcgggttatt caacggacgc cagatccatt cgccattacc ggcatggata
     1621 gacagaccgt tagagtcgtg caactccgga cgatagttat ttgcaggcga cggttggttc
     1681 ggcccaaaca ggaacatact ggttaacggt gcaaccccca gtttgccgac tttatcgcgc
     1741 agatagattt tcgactgcac atccacaacc gtgtcacgcc ctggcataac cacgaatttg
     1801 taagcacctg tcgcgcgcgg cgagtcaagc aatgcataaa tggttaaacg tttatcagtc
     1861 ggttttggac gctcgatcca gaactctttg aagcgtggaa attcttcacc cgatggcaag
     1921 gcggtatcaa ttgccaggcc gcgggcagaa aggccataaa cctgacctgc accaatcacg
     1981 cggaaatagc tggccccgag catgctgacg atttcatcgt ttttatcttt gctgttgatc
     2041 gggtaaagca ctttaaaacc ggcaaaacca aggtctttta ccgtgtcttt gtcatgctga
     2101 acatcgccga aagtgaaata atccgggctg tatttgattc gtttgactgc ggtggcagtc
     2161 acttcattta ttttgaccgg ggtatcgaag tacataccct gatggtagaa ctcgagtttg
     2221 aatggggtct tcagattgtt ccagtacgct ttgtcatgat taaactggat ctgctgatag
     2281 tccgcgtatt tcatatcgcg gaaaacggag ggcaagttgc ttttgggcgc ctcatagcct
     2341 ttcccggcta aggattgagc ttgctttgcg acatcatcaa tactgaaagc ccagcttgaa
     2401 gatgtataca gggttaacat tactgcagca ctcaaccaac gcattttcat catttgtagt
     2461 ttatgtttca taattagtaa gcacttcccc ctttgtgtgc ttatatcgat ccgatctatt
     2521 ttaatggata accagccatt ccgacaacag aatcccggta tcgttcagca cgctggctca
     2581 tggattaagc acctgattgc aaccgttttc actttccgtg cttctcctaa agacagaagc
     2641 atcgacttcg tgtagggttt gccagcaatt ttatttatca accgtcttac agacgcttcc
     2701 attctggtta gtggtagcga tgatgctatg cataaaaacc ttgaagcctg cgcctggcgc
     2761 ggtcaggtcg gtgtcgctat ggcaacaagc ctaaaggcat aacccgacag ggataagacg
     2821 aaaaatccga gattagttaa ccatatatga atccagtacc cgcgcaacgt gaatatttcc
     2881 tcgactccat ccgcgcctgg ctgatgttgt tagggatccc ttttcatatt tctttaatct
     2941 attcgagcca tacatggcat gtgaatagcg ccgaaccatc attgtggctg acccttttta
     3001 atgacttcat ccactcgttc cgcatgcagg tatttttcgt tatatccggc tacttttcct
     3061 acatgctttt tttacgctat cccttgaaaa aatggtggaa agtacgtgtc gaacgtgtag
     3121 gtatcccgat gttaacagcc atccccctac tgacattacc gcaatttatt atgctgcaat
     3181 atgtcaaagg aaaagcggaa agttggcctg ggctgtcatt gtatgacaaa tataatacgt
     3241 tggcctggga attaatatca cacctgtggt ttttactggt gttagtagtc atgacgacgc
     3301 tgtgcgtatg gatatttaag cgcatcagaa ataatttaga aaattctgat aaaacgaata
     3361 aaaaattctc gatggtaaaa ctatcggtga tttttttatg cctcggcatc ggttatgcgg
     3421 taataagaag aacgattttt attgtgtatc cgcccattct gagtaatggc atgttcaatt
     3481 ttattgtcat gcaaacgctg ttttatttgc cgttctttat cctcggcgca ctggctttca
     3541 ttttccctca tcttaaagcc ttgtttacca cgccgtctcg tggctgtacc cttgcagcag
     3601 cattggcgtt tgtcgcttat ttactcaacc agcgctatgg cagtggcgat gcctggatgt
     3661 acgaaaccga gtcggtgatc accatggtcc tcggtctgtg gatggtgaat gtggtcttct
     3721 cctttggcca ccgtttgctt aacttccagt cagcgcgggt gacttatttt gttaacgcat
     3781 cgctgtttat ctatctggtt caccacccgt taacgctgtt tttcggcgca tacattacac
     3841 cgcacatcac ctccaactgg cttggttttc tctgtggcct gatatttgta gtagggattg
     3901 cgataattct gtatgaaatt catttgcgca tcccgttact gaagtttttg ttctctggta
     3961 aaccggttgt taagcgtgag aacgataaag caccagcccg ttaagccaca tttacaataa
     4021 ccattccacg ggcaatatcg acgccagtct gaccataacc cgcttccaga aactggtggc
     4081 gggttctttt ttgagaataa tctctttctt tgcatgacga tcaacccagt tgatccgtcc
     4141 ccacctgtcc agacggagct gccaggccgc atcatactgg ctctgaataa agcgtttatc
     4201 aattaactgt gccagcgttt cgctctctat cacgaagccc atttcagtat tgagcaatgt
     4261 tgaacgcgga tcgaaattga aagagccgat aaacaccgtt ttaccatcga tgctaaaggt
     4321 tttagcatgc aggctggctc cggaattacc ggttatgccg cgatcgtgta acgtactact
     4381 ttgttcacgc gtcggcttga gttcatataa ttccacgcca tagcggagca attttttgcg
     4441 ccagcgcgcg tatccggcat ggacgacagc aacatcgtta gcggcaagag aattggttag
     4501 gatcgcaatc tttacccctt ttctcaccat ccgtaagagt tgcgccacac ctgcgcgtgt
     4561 cggtacaaaa taggaagaga taatatcgat gcgttcactg ggtgagccca tgatatcgaa
     4621 caggcgctgc ggtaacagtg aatgccgttt tgccttgccc tcccctttcg ccggatcatc
     4681 acttaataaa cgtgtcttcg cccagataag cggcaatgtt ccatcaacca gatgatttat
     4741 aaatggactg gattccattt tgcgtaaata acgatgcgtc atggcatcgt tatgccagga
     4801 ggcgggtaac tcgatgcgat ccgccatttc accctccggg acatccagca cctgctgtaa
     4861 gggtgaaacc gatttgcaat accagtagcg ggcgaaatca tcggcaacgt cctctaccac
     4921 gggtcctatt gccatgacat ctaaatccga aaaaagtggc tcctcccctg ctccaaaata
     4981 ggcatcacca atatttcgtc ctcccaccag ggtcaccacg ccatcgacag tgaaactttt
     5041 attgtgcata cggcgattaa gacgggaaaa gtcggtgata taaccaagcg gacgcagcaa
     5101 gcgaaacgag aaaggattaa aaagccggac ttcaatgcgt ggatgactgt caagcaagcg
     5161 taaaatgtcg tcaagtccgg gcgtattgtt gtcgtccagc aacaaacgga cgcgaacgcc
     5221 acgctttgct gcggctaaca gggcggaaaa cagtaaccga cccgacatat cgtcctgcca
     5281 gatgtaatac tgaacatcga gcgtatgctc tgccatttcg gccagacgat accgggcggc
     5341 aaacgcatcc agacttttct ccagaggaaa aaggccacac tgaccgggat gttgcgaaca
     5401 cagtggcagc accgcgctcg ccagccgggg caaatcattc atctccttgt tgggtaagga
     5461 gtctttcgta gaggtgggcg ttttcttcat cataacagac aaagtatacc tgttcgggta
     5521 aagcgtgacg ggtaataaat tctgaaacgg tttttactgc gatttcagcc gctgccgcac
     5581 gagggtaacc ataaacccca gtactgattg caggaaaagc cactgacgta tagctgtttg
     5641 ccgccaccag tcgtaggcta ttgagatagg catcctgcaa aagctggtct tcgttttgtt
     5701 caccaccacg ccagactggc ccgacggtgt gcactacggc tttagcggga agagcgcctg
     5761 caagcgtaat aacggcatgc cccgtagggc aatcgccctg ctgttgcctg acttttaaac
     5821 aggcatccag tagggccgga cccgctgcgc gatgaatggc cccatcgacg ccgccacctc
     5881 ccattaatga aggattagcc gcattcacaa tcacatcaac ggccagtttg gtaatatcac
     5941 cctgcacaac atgaatacgc gttttcataa cgcctcctta caccttcttg tttcttaagt
     6001 gtacagcagg ctttttggtt aaatcgcatt tttctactca gcaacgtgag ttgttcagaa
     6061 acttagttat attcgatgtt cattacagcc agctttttct gtttatcaag atactgcact
     6121 tttactgagg ctatttgcgc gtttttgaca ttgccgctca ttaacgcttt gctggaatag
     6181 gttttgcccg gaacagaacc attctgtggg cagttcattt ctacgggtga tgactgggtg
     6241 ctgacgtcac atgccggttc cacaatttgg ccataaaaat ggatcacgcc cccttgcgcg
     6301 gtgcttcctg cgatggcaat aaaaggaaaa aacaaactgc cgagcataag agagtttaaa
     6361 aaaggtctga acatttattc tatttcctca atgattagtc atccttgagg gttgtgttat
     6421 ccatactttc aggttggctg attcgcagca gaccattctc tccagattca tcttatgctc
     6481 gatatttcaa caaattaaga cttttctgaa gagggcggcc attgttgtga taaatgaagt
     6541 gactgtccat cagaaacagt aacaactatt ttcacccgat cgtccgggga aatatttaaa
     6601 ctcaacttcg tcaaagcaat gggttgatta gcaggcaatg aaagggtctt ttcttgcttc
     6661 gtctgacttt gccctgaact gccttcgcgc agggacaata tttgtactct gcacagacaa
     6721 gattgagtaa gagtgacttc aggaataatg gtatacacat ccccttgctg ggtcgtatta
     6781 aaggttatct gactggaaag tgccgcaagg agtaataacg cattcatatt cttctcccga
     6841 aaaaaaacag ggcttgcgcc ctgtttctgt aatacaaatg atgtattagt actgatgagc
     6901 ggtcgcgttg ttaccaaagc caacctgagt cacgttgacg gaggagttag atgcagtctg
     6961 gtcaactgca gcaccgttgc caccaccgaa ctgtttaacc gtcatttcag aatttttgcc
     7021 gttccactga tcaagagtag cgctgttacc gaagccacgt tgggtcagat cgattgagct
     7081 gtcatccgag ccctgaccaa catctgcacc attaccgccg ccatgctggg taatagtcaa
     7141 gtcagagtta cgggcatcag tttgcagagc aagtgcagag ttaccgccac cgtactggta
     7201 aatgttcagc tcagaatttg ggccgctatt attaccgcca ccaccgtggt taccgccgcc
     7261 gccgtactga ggaacaacac ctgccagagc gctaccggag aatacgattg ctgcaattgc
     7321 tgctactttt aaaagtttca tgtaaaaccc ccatcggatt gatttaaaag tcgaatggaa
     7381 attaacgttg tgtcacgcga atagccattt gcgactgtct ctgcactaca attgccgttt
     7441 tttgagtacc atactgtgta atatttgctt tattaccaga acctttctgg ataatcatcg
     7501 cagtattacc ataagcacct tgcgaaatac tggcatcgtt ggcactgccc gcctgatcaa
     7561 tatatgcaag gttataatct cctgtctggt caatctttgc ccggttgcta ctaccttctt
     7621 gcgcaacaac cgccaaaagt tttgagcctc cctgccgtaa ctgagcacta ttattcgtcc
     7681 cagcttgacc aattatggct gcctgattaa atgaagactt actcaattca tttaccgcga
     7741 agttatattc tgaattagct aaatcataac ctgctgcggc tgcaatccca ggcgcaccca
     7801 gtattgttaa catcataaat aacaatttgt ttttcatgtt gtcaccctgg acctggtcgt
     7861 acatttaaga aattaaatca tttcaacttg gttgttaacg caacctgtat tttgttaacg
     7921 ctgcgttacg atggaaagta tgtctgcgga aatattttta ataactcacc cgcgcgttgt
     7981 atttttcttt ttttgttcat acaaaagtat taaaacccac gtaaatacgc tgttatctac
     8041 gcaaaaaata ttttgttttc ttttaaatct ccgttttccg ctatcaaaaa tcaccagaca
     8101 gtcatttttc ttgcccgtcg ctgattgctg cgatatgtct tgcgcacaag ccgtgacaaa
     8161 cctcgtcttt ttgcagtaaa aaattgtcca cggaggtgcg gagaaaaaac aagaacgttt
     8221 tacatgacga aaggactaca ccgaaatatt ttttatatgt attattagta agttatcacc
     8281 atttgtatga ttttttaaaa ttgtgcaata aaaaccaaat gtacaacttt tctatcattt
     8341 ctaaacttaa taaaacctta agattaacat tttaatataa cgagttacat ttagttacat
     8401 gtttaacact tgatttaaga tttgtaatgg ctagattgaa atcagatgta atccattagt
     8461 tttatatttt acccatttag ggctgattta ttactacaca cagcagtgca acatctgtca
     8521 gtacttctgg tgcttctatt ttagaggcag ctgtcaggtg tgcgatcaat aaaaaaagcg
     8581 gggtttcatc atgtttaatg aagtccatag tattcatggt catacattat tgttgatcac
     8641 taaaccgtct ttgcaagcga cagctctctt gcagcacctt aaacaatcgc tggcaattac
     8701 aggaaaatta cataatattc aacgttctct ggacgatata tcttcaggct ctattattct
     8761 tctggatatg atggaagcag ataaaaagct tatccattat tggcaggata ctttgagcag
     8821 gaaaaacaac aatatcaaaa tattgttgct aaatacgcct gaagattacc cgtaccgcga
     8881 cattgaaaac tggcctcata tcaacggcgt tttttatgcc atggaggatc aagaacgtgt
     8941 tgtcaatggg ttgcaaggcg tcctgcgcgg cgaatgctac tttacgcaaa agcttgccag
     9001 ttacctgatt acacattctg gtaactatcg ttataacagc acggaatcag ccctccttac
     9061 tcatcgggaa aaagagatcc tgaataagct gcgtatcggc gcgtctaata acgagatcgc
     9121 tcgttcgttg ttcatcagcg aaaatacggt taaaacgcat ctttataatc ttttcaagaa
     9181 gatagccgta aaaaaccgga cacaagcggt ttcctgggca aacgataacc tcaggcgata
     9241 aagccatgaa acgttattta cgctggattg tggcggcaga atttctgttc gccgcaggga
     9301 atcttcacgc cgttgaggta gaagtcccgg gattgttaac tgaccatact gtttcatcta
     9361 ttggccatga tttttaccga gcctttagtg ataaatggga aagtgactat acgggtaact
     9421 taacgattaa tgaaaggccc agtgcacgat ggggaagctg gatcactata acggtcaatc
     9481 aggacgttat tttccagact tttttatttc cgttgaaaag agacttcgag aaaactgtcg
     9541 tctttgcact gattcaaact gaagaagcac taaatcgtcg ccagataaat caggcgttat
     9601 taagtacggg cgatttggcg catgatgaat tctaaataaa aaattgttcg gaggctgcaa
     9661 tgcgtgtcaa acatgcagta gttctactca tgcttatttc gccattaagt tgggctggaa
     9721 ccatgacttt ccagttccgt aatccaaact ttggtggtaa cccaaataat ggcgcttttt
     9781 tattaaatag cgctcaggcc caaaactctt ataaagatcc gagctataac gatgactttg
     9841 gtattgaaac accctcagcg ttagataact ttactcaggc catccagtca caaattttag
     9901 gtgggctact gtcgaatatt aataccggta aaccgggccg catggtgacc aacgattata
     9961 ttgttgatat tgctaaccgc gatggtcaat tgcagttgaa cgtgacagat cgtaaaaccg
    10021 gacaaacctc gaccatccag gtttcgggtt tacaaaataa ctcaaccgat ttttaagccc
    10081 cagcttcata aggaaaataa tcatgcagcg cttatttctt ttggttgccg tcatgttact
    10141 gagcggatgc ttaaccgccc cgcctaaaga agccgccaga ccgacattaa tgcctcgagc
    10201 tcagagctac aaagatttga cccatctgcc agcgccgacg ggtaagatct ttgtttcggt
    10261 atacaacatt caggacgaaa ccgggcaatt taaaccctac ccggcaagta acttctccac
    10321 tgctgttccg caaagcgcca cggcaatgct ggtcacggca ttgaaagatt ctcgctggtt
    10381 tataccgctg gagcgccagg gcttacaaaa cctgcttaac gagcgcaaga ttattcgtgc
    10441 ggcacaagaa aacggcacgg ttgccattaa taaccgaatc ccgctgcaat ctttaacggc
    10501 ggcaaatatt atggttgaag gttcgattat cggttatgaa agcaacgtta aatcgggcgg
    10561 tgttggggca agatattttg ggatcggtgc agacacgcaa taccagctcg atcagattgc
    10621 cgtgaacctg cgcgtcgtca atgtgagtac cggtgagatc ctctcctcgg tgaacaccag
    10681 taagacgata ctttcctatg aagttcaggc cggggttttc cgctttattg actaccagcg
    10741 cttgctggaa ggggaagtgg gttacacctc aaacgaaccc gttatgctgt gtctgatgtc
    10801 ggctatcgaa acaggggtca ttttcctgat taatgatggt atcgaccgtg gtctgtggga
    10861 tttgcagaac aaagcagaac ggcagaacga cattctggtg aaataccgcc atatgtcggt
    10921 tccaccggaa tcctgacaga taaaaaagcc ggaattatca tccggcattt tactgtttaa
    10981 gcgaaagcgt gagtgcccct cacgctttat ttttgatttc ctgctcacgg tgagccgtct
    11041 ttgcactacg cgccgccagc cacaggccgc tgtttttcat tgcgtaacca aataccagcc
    11101 caagcgccag cgaaggtaac accagtttcc agtcaccctg cccggcaaac gtcgcgcatg
    11161 cgcctataaa cgtccccgga acaaatgaaa gcagcagctg tttggcctga atacacatca
    11221 gaaacgccac aataccggtt atgacataac cgagaatttc cagatgtggt gccagtgcac
    11281 taccgtaaat aatgaccatc gcccacacca cgccactaag cagggttgca gcggagatcg
    11341 ccagcccttt cagcccacct tgcgggcagg caaagtaggc cgtacagccc aggaagcccg
    11401 cccagcttag taagcccagg gaaacagcca cccatcccca gataccggag agaatgcctg
    11461 ttgtgattgc aatagagaga agtatgttca tgccgcgcat gatagcagaa aacgcggcca
    11521 tgaatgagat cgagcacaca ttttatgcaa caacaaatta tctgttgcag ttatgagtga
    11581 cgatgatcac atttactctt cagaatcttc ttccagctcg tcccacattg cactaatggc
    11641 atcacgggtt agcggtgcca tggtgcgcca gaaaggcgtg gttgcatggg cctccacttt
    11701 gccgaggaac gcaccacacc agggtaacag atactcactg aacagtgttt ccagtgcttc
    11761 gctctcatct tccgttgact gatcttccag ccaggaagcc gcaagcagca atgtgccgat
    11821 gtgatcggct ggcgtatccg ccagtggcat ccctcgttcg gaaagaaaag cgcgcacttc
    11881 cgcttccgtt gcgtcctcaa cccatgcgct acgatatggc ggcacggcac attcatcgcc
    11941 gataaacaac gcattgtaat cggcagagac ttgcgccata tcacagcttt tctgtaaacg
    12001 cgtcagtaac tcatcctgct ccagtggcca gttcgcagcc agtttcccct cacgaatcag
    12061 ggtaaacagc ggcaccagta aaggatcttg cggttggcgg taatagagcg aacccagcac
    12121 acgacagagg atagaaaact cgttcattaa tatatttcca ttactagtaa ttaaaggtct
    12181 gcaaattccg caatcggtgc cataccgcga gattcaagga agttcagtaa gcggcgcgga
    12241 gaaacattca aaatgcgctc tggcggaaaa tctaccgcgt cgaggatttt aagacactct
    12301 tcaaattccc ccatggtaaa cgcggtgtga gaatccgagc ctaatgccac ccaacctcca
    12361 gcatcacgta ccgctgcggc taccgcacga cagttgtctt cactgccctt acgtgagtgt
    12421 aaaaatgaag aattattgat ttccagcgcc acctgatgtt tcgcggctgc ttcagcaacg
    12481 gctttcacat ctatttcata tttgggattt ccgggatggc ttattatatg cacattgccg
    12541 ctggcgatag tggcgatcat cgcttgtgta ttggtcgctt tgtcatgtgg tgcaaaaacc
    12601 ggctcatgaa aaccggcaat aatcagatcc agcgagtcaa acattttacc gctgcaatca
    12661 atttcaccat caacattttt aatgttagct tcgatgccgc gcaggatccc taccccatca
    12721 accactcgcg gccagatacg catgttaatg aagtgccagt gatgcggagc atcttccata
    12781 tccgggccat gatcggtgat cgcaaaaagt ttaatgccct tttgtttggc ctgggcaatg
    12841 taatcactca atgtgctata tgcatgtgtg ctggcaacgg tatgcatatg aaggtcgacg
    12901 ggatacataa ctctctcctg tattcaattc ccgccaagga tagcaggaat cctgatgctt
    12961 tattagtagc cgcgtgcgcg gtcgacttgc ccgcagacct tctccccttt ttcgagctgg
    13021 gcaatagtgc gagaaatgta ctccacagct tcagcgggac gggtaatcgc ggcgacatgt
    13081 ggtgttatcg tcacgcgtgg atgttgccag agcggacttt caggcggtaa gggttcacga
    13141 ttaaaaacat ccaagcatgc gcctttaact ttcccgctat ccagcgccgc gagcaggtca
    13201 tcttccacaa catgaacacc acgcgccagg ttgaggagat acgcgccatc cggtaatttt
    13261 tcgagtaatt gttgattaat aatgccgacg gtttcagggg tattcggtaa caaattaatc
    13321 aatacccgac attggctcag aaatgcagac agttcttccc gtccggcaaa gctttgcacg
    13381 ccaggccacg atttacgggt tcgactccag caacgcagcg gaaagcgcca ggtttgcaga
    13441 ctctgagcaa ctttactgcc cagtacgcct gcgcccaaaa tgccgatggt aaaatcttcc
    13501 cgatgatatt ccggcagcgg ttgccaatgc gaactatttt gctggatgcg ataatcgtca
    13561 aaacgtcgaa accaatgcag cacctgactg acagcatatt cctgcatttg ctcgcccata
    13621 ccggtatctt ccaggcgaaa aagtggaaca gaagggttca gcatttcagg gtgtgcctgt
    13681 agcttgctca aaatagaatc aacaccggcc ccgagtgcga acaccgcttt aagatcgcgc
    13741 cctgccagca tttcaacagg aggatgccag actaaagcat aatcagcaga gtcattatct
    13801 ccgcttttcc acgctctgac tcttgcctga ggaatagctt tgcgcagtgc ctcaatccac
    13861 cattgggtat cgaacgttgg gtgataaaag atgatatcca tactgactcc cgaaaagcgt
    13921 tgtgcgaaat ttattcgcac ttatcgttat gatctacaaa ggccaccagc ataacaaatc
    13981 cgtggtcggt ggcaaaaaaa gcagatttcg cttattaaaa ccacacattg attgaagttt
    14041 gaataaacgc gcgatttttt caaaaagttt gttgacctca ggtcatgatt tccctaaatt
    14101 agcgcccgtt ccagcaagac aggaacgaca atttggtgag gtgtccgagt ggctgaagga
    14161 gcacgcctgg aaagtgtgta tacggcaacg ta
//
LOCUS       DAMZET010000109        14119 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.109, whole genome shotgun sequence.
ACCESSION   DAMZET010000109 DAMZET010000000
VERSION     DAMZET010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.109"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..285)
                     /locus_tag="POB34_003255"
     CDS             complement(<1..285)
                     /locus_tag="POB34_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HDI5920147.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(404..1291)
                     /locus_tag="POB34_003256"
     CDS             complement(404..1291)
                     /locus_tag="POB34_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDI5920148.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            1448..2488
                     /gene="gpr"
                     /locus_tag="POB34_003257"
     CDS             1448..2488
                     /gene="gpr"
                     /locus_tag="POB34_003257"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HDI5920149.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(2528..3022)
                     /locus_tag="POB34_003258"
     CDS             complement(2528..3022)
                     /locus_tag="POB34_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HDI5920150.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            3213..4097
                     /gene="yghA"
                     /locus_tag="POB34_003259"
     CDS             3213..4097
                     /gene="yghA"
                     /locus_tag="POB34_003259"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDI5920151.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(4369..4794)
                     /gene="exbD"
                     /locus_tag="POB34_003260"
     CDS             complement(4369..4794)
                     /gene="exbD"
                     /locus_tag="POB34_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HDI5920152.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(4801..5535)
                     /gene="exbB"
                     /locus_tag="POB34_003261"
     CDS             complement(4801..5535)
                     /gene="exbB"
                     /locus_tag="POB34_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDI5920153.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            5787..6974
                     /gene="metC"
                     /locus_tag="POB34_003262"
     CDS             5787..6974
                     /gene="metC"
                     /locus_tag="POB34_003262"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HDI5920154.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            7114..7773
                     /locus_tag="POB34_003263"
     CDS             7114..7773
                     /locus_tag="POB34_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDI5920155.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(7813..8712)
                     /gene="yqhC"
                     /locus_tag="POB34_003264"
     CDS             complement(7813..8712)
                     /gene="yqhC"
                     /locus_tag="POB34_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HDI5920156.1"
                     /translation="MKREEICRLLADKVNKLKIKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            8906..10069
                     /gene="yqhD"
                     /locus_tag="POB34_003265"
     CDS             8906..10069
                     /gene="yqhD"
                     /locus_tag="POB34_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDI5920157.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWGATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            10174..11001
                     /gene="dkgA"
                     /locus_tag="POB34_003266"
     CDS             10174..11001
                     /gene="dkgA"
                     /locus_tag="POB34_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HDI5920158.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            11201..12127
                     /locus_tag="POB34_003267"
     CDS             11201..12127
                     /locus_tag="POB34_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HDI5920159.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            12178..12435
                     /gene="yqhH"
                     /locus_tag="POB34_003268"
     CDS             12178..12435
                     /gene="yqhH"
                     /locus_tag="POB34_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HDI5920160.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(12478..>14119)
                     /locus_tag="POB34_003269"
     CDS             complement(12478..>14119)
                     /locus_tag="POB34_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDI5920161.1"
                     /translation="FGNGERPLVEVAHRLAMGEPISEIRDVRNTAIIVKEALPGWSGV
                     DSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKAVTVQPPRPKPWEKTYVLLP
                     SFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRYVWINPPAIPLSTEEMDSVF
                     ALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSFCSITEHEGRIIQSRSEDSI
                     INEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAEQTCRRLSCVYPDICPHMDT
                     NHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYIKELATHHVGGYLKIAPEHT
                     EEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPYFISAHPGTRDEDMVNLALW
                     LKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYKSEDVFVPKGDKQRRLHKAL
                     LRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIEEMREARRQNRNTRPALTKH
                     TPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
BASE COUNT         3554 a         3648 c         3543 g         3374 t
ORIGIN      
        1 attttcgagg cgataaccgg ccataaaaaa gcgcaacgta gaacaggaat tattctcacg
       61 aaagaaatcc attaccatct ctttatccag tccatagcgg cgcgtcagaa tacccgcttc
      121 ccaggcgcta agctggcgat ctccggtctt ttcgtacatc cggtgtgagt agcctaatac
      181 aaaaccgcgt ttataatcgg cacagaattt tgtcacatta accgcactgt cggcatgcgt
      241 tgcttttaat cccgccatta agcctttacc aaaatggtta ttcatcgttc acctcattcg
      301 ctatatatcg ccatatatag cgatatttta ggcaaagaac gacttttgcg cacgctattt
      361 tttcatgctt gtttaagacg taatccagta agagataaac ctatcaggag agatatttat
      421 cgaaccattt cagtgtcctt tgccaggcaa gatcggcggc agatttgtca taacggggcg
      481 tggaatcatt atggaatccg tgattaaccc ccggatagat atacgcctca taaaccttat
      541 tattggcttt caacgccgcc tcgtaagcag gccagccctc gttgattcgg gtgtccagtt
      601 ccgcgaagtg gagtagtaaa ggcgcttcaa tcttcgccac atcggcagtg ggtgcctgac
      661 gaccataaaa cggcaccgcg caggccagtt ccggatacgc gacagccgcc gcgttcgata
      721 cgccaccgcc atagcaaaat ccggtaatac ccactttgcc tgccgcttgc ggatagcgtt
      781 gcataaactc aattgcggca aagaaatcat tcatcagttt ggttggatca acctgctgtt
      841 gcagctcacg acctttatca tcatttcccg gataacctcc aacggaactt aagccgtcag
      901 gtgccagggc gatatacccc gccttcgcca ctcgccgtgc cacatcttcg atatacggat
      961 tcagtccacg attctcatgc accactacca cggctggcgt tttgccgctc atttttgcgg
     1021 gtttcaccag ataaccccgt acctcgccgt gaccatttgg cgaaggatac gtgatgtact
     1081 cagcaacaat ctccgggtcg gtaaactcta cctgagtcgc cagcgcataa tttggcttga
     1141 gcaaatcaaa caacgctaat gccgtcatcc cgcccaccgc atacttcgcc gcaaggttga
     1201 ggaactcacg tttcgagatt ttcccgtgag cgtaatagtc gtagtaatcc aacaactctt
     1261 gtgggaaatc tttggcggtt aaacgcggca tcgttgcact cctcaactgg tgttttttta
     1321 agcaaagcat aagcacgtgt ttttgcccag tttttcgtca ctctgtgagc cagactacgg
     1381 gatacgcgct ggcgaatcgc taaactagaa acattgtttc gaaattgaac ggtggaaagg
     1441 agaggtcatg gtctggttag cgaatcccga acgttacggg cagatgcaat accgctattg
     1501 cggaaaaagc ggtttacgcc tgcccgcgtt atcgctcggt ttatggcaca atttcggtca
     1561 cgttaacgcg ctggaatcac agcgtgcgat cctgcgtaaa gcgtttgatt tgggcattac
     1621 gcactttgat ttagccaaca attacgggcc gcctccagga agcgcagaag agaactttgg
     1681 tcgcctgctg cgggaggatt ttgccgctta tcgcgatgaa ctgattatct ctaccaaggc
     1741 tggctacgat atgtggcccg gcccttacgg ctctggcggt tcacgtaaat acctgctcgc
     1801 cagcctcgac caaagcctga agcgtatggg gcttgagtat gtcgatatct tttactctca
     1861 tcgcgtcgat gaaaatacgc cgatggaaga aaccgcctct gcgctggctc atgcggtaca
     1921 aagcggtaag gcgctgtatg tcgggatctc ctcttactcg ccagagcgga cgcaaaaaat
     1981 ggtcgagttg ctgcacgagt ggaaaattcc gcttttaatt catcaacctt cgtacaattt
     2041 actgaaccgc tgggtggata aaagcggcct gctggatacc ctgcaaaata acggcgtggg
     2101 ctgtattgcc tttactcctc tggctcaggg attgctgacc ggaaaatatc tcaacggcat
     2161 tccgcaagat tcacggatgt atcgtgaagg gaataaagtt cgtggtctga caccgaaaat
     2221 gcttaccgaa gccaacctca acagcctgcg cttattgaat gaaatggcac agcagcgtgg
     2281 acaatcaatg gcgcaaatgg cgttaagctg gttgttgaaa gatgatcgcg tgacgtcggt
     2341 attgattggt gccagccgcg cggagcaact agaggagaac gtgcaggcgc tgaataatct
     2401 gacatttagc accgaggagc tggcgcagat tgatcagcat atcgccgatg gcgagctgaa
     2461 tctgtggcag gcgtcgtccg ataaatgacc tgttaataac gggcgcgcga accgcgcccg
     2521 aatcagatca gtgattatga cgagtcagtc ggtcaagata gcccatcaca aatgcagaaa
     2581 gcacaaacgt cagatggata atgacgtacc acatcagttt gttatccggg acatttttcg
     2641 catccataaa gacgcgcagt aggtgaatgg aagaaattgc cacaatcgac gctgctactt
     2701 tgtttttcag cgacgttgcg tccattttcc ccagccagtt cagcttctct ttgttctcgg
     2761 agatatccag ctgtgagacg aaattctcat aaccggaaaa catcaccatc accagcaaac
     2821 cgccaaccag cgtcatatcc accagcgaca gcaacacgag gatcaaatct gattccgcca
     2881 tcgagaagat attcggcagt acgtgaataa tctcctggaa gaacttcagc gccagggcaa
     2941 ctaacgccag cgaaaggcca aagtacacgg gggcaagcag ccagcgagaa gcatacattg
     3001 cattttcaag aaaacgttcc atacattcct gtcgtgtagt taaaacgggc aacagtatat
     3061 cgcaattgag taaacagcac gcaacagcaa atcaacatat acgttaaacc tttgtcgcat
     3121 aattctgagg tttagggatc ttcctggttc ccttatcaac gcatcgagag cgtctaccct
     3181 taaaggacgt tgaacctgaa gggagaaaaa cgatgtctca tctaaaagac ccgaccacgc
     3241 agtattacac tggtgaatat cccaaacaga aacaaccgac gccaggcatc caggcgaaga
     3301 tgacaccggt accggattgc ggcgagaaaa cctatgttgg tagcggtcgc ctgaaagatc
     3361 gtaaagcact ggtgacaggg ggcgattccg gaataggtcg cgctgccgcc atcgcttacg
     3421 cgcgtgaagg ggctgacgtg gcgatcagtt atcttcccgt ggaagaagaa gacgctcagg
     3481 atgtgaaaaa gatcattgaa gaatgcggtc gcaaagccgt tctgctgcca ggcgatttaa
     3541 gcgatgagaa attcgcccgt tcgctggttc acgaagcgca caaggcgtta ggcgggctgg
     3601 atattatggc gctggtcgcc gggaaacagg ttgccattcc tgatattgca gacctcacca
     3661 gcgaacagtt tcaaaagacc tttgccatta acgttttcgc gctgttctgg ctaacccagg
     3721 aagcgatccc cctgctaccg aaaggtgcaa gtatcatcac cacttcgtca atccaggcat
     3781 accagccaag tccgcattta ctggactatg cggctacgaa ggcggcgatt ctgaactaca
     3841 gccgtggcct ggcaaaacag gtcgcggaga aaggtattcg ggtgaatatt gtcgcgccag
     3901 gccctatctg gacggcactg caaatttctg gcggacaaac gcaggataag atcccgcagt
     3961 ttggtcagca aacgccgatg aaacgtgcgg ggcaaccggc ggaactggcc cctgtatatg
     4021 tttatctggc aagtcaggag tcgagctacg tcaccgcaga agtgcacggt gtgtgcggcg
     4081 gcgagcattt aggttaaaaa aatgcccggt tgtgaaaagc aaccgggcat cattgtgaat
     4141 tcactcttac ctgatgtggc gaaaacgcct tatccgccta caaacgcatg aagattcaac
     4201 atgttgcaat agattttgta ggcctgataa gcgtagcgca tcaggcattg agcacctaat
     4261 gccggatgcg gcgtgaacgc cttatccggc ctacaaacgc acgaagattc aacatgttgc
     4321 aatagatttt gtaggcctga tgagcgtagc gcatcaggca attctacttt acttcgcttt
     4381 ggcggtttct tcgccgacca gacctatctt caggtaaccc gcctgatgca gcgtatccat
     4441 taccttcatc aacgtctcgt aatcgacggt tttatccgct cggaagaaga tggtggtgtc
     4501 tttcttgcct tcggttaacg cattcaacgc cgtaatcatt gtttcatcgg tgaccggatc
     4561 gttaccgata aacatcgagt tgtctgcctt caccgacaga taaaccggtt tttccgggcg
     4621 cggctgcggc gtgctggtag aagcaggcaa gttcaccttc acatctaccg tcgctaacgg
     4681 tgccgccacc ataaagataa tcagcagaac caacatcacg tcgataaacg gcgtcacgtt
     4741 gatatcatgc atttcgccgt tatcgtcgag gttttcgtta agatgcattg ccatcggata
     4801 ttatcctgcg cgtaattttt gtgcgacacg aaccggatgc gcagcggcgc tggcttccag
     4861 atccaggtca cggctttgca gcaacaatac ctgcgctgca acatcaccca gcatcgcttt
     4921 aaagccgcca atctggcgtg caaatacgtt atagataacg accgcaggaa tcgctgccac
     4981 gaggccgatt gccgttgcta acagagcttc tgcgataccc ggcgcaacga ctgccaggtt
     5041 agtggtctgc gtttgcgcga taccaataaa gctgttcatg atgccccaga ccgtaccaaa
     5101 cagaccaacg aacggagaaa tcgcgccgat ggttgccaga tagccgttac cgcggcccat
     5161 ttgacgaccc actgcggcga cccgacgctc cagacggaag ctggtacgtt ctttaatacc
     5221 ttcgttatcg tcgctgcctt ctgacagttc cagctcattc tgcgcttcat tgagcaaatg
     5281 caggcttaag cttttgctac caaaatcagc ggcgatatcg ttggcctggt ttaaggaacg
     5341 cgcttcagcc agcagttgct gctcgcgctt aaggcgacgc ttctgattga agaactctac
     5401 gctcttactg aagaagattg cccaggtgac tacggaggcc aaaataagcc caatcatcac
     5461 gcacttaacg acaatatcgg cgtgctgata cataccccag acggaaaggt ccgtctgcat
     5521 taaattatta cccactgtgt atctccagga cgcaagtcac aaaatctgcg cataataata
     5581 tcaaaacgac gtcgaattga tagtcgttct cattactatt tgcatactgc cgtacctttg
     5641 ctttcttttc cttgcgttta cgcagtaaaa aagtcaccag cacgccattt gcgaaaattt
     5701 tctgctttat gccaattctt caggatgcgc ccgcgaatat tcatgctagt ttagacatcc
     5761 agacgtataa aaataggaat cccgacatgg cggacaaaaa gcttgatact caactggtga
     5821 atgcaggacg cagcaaaaaa tacactcttg gcgcggtaaa tagcgtgatt cagcgcgctt
     5881 cttcgctggt atttgagagt atggaagcca aaaagcacgc gacgcgcaat cgcgccaatg
     5941 gcgagttgtt ctatggacgg cgcggaacgt taacccattt ttccttacaa caagcgatgt
     6001 gtgaactgga aggtggcgca ggctgcgcgc tatttccctg cggggcggca gcggttgcta
     6061 attcgattct tgcttttgtc gaacagggcg atcatgtgtt gatgaccaac accgcctatg
     6121 aaccgagtca ggatttctgt agcaaaatcc tcagcaaact gggcgtaacg acatcgtggt
     6181 ttgatccgct gattggtgcc gatatcgtta agcatctgca gccaaacact aaaatcgtgt
     6241 ttctggaatc gccaggctcc atcaccatgg aagtccacga cgttccggcg attgttgccg
     6301 ccgtacgcag tgtggtgccg gatgccatca ttatgatcga caacacctgg gcagccggtg
     6361 tgctgtttaa ggcgctggat tttggcatcg atgtttctat tcaagccgcc accaaatatc
     6421 tggttgggca ttcagatgcg atgattggca ctgccgtgtg caatgcccgt tgctgggagc
     6481 agctacggga aaacgcctat ctgatgggcc agatggtcga tgccgatacc gcctatataa
     6541 ccagccgtgg cctgcgcaca ttaggtgtgc gtttgcgtca acatcatgaa agcagtctga
     6601 aagtggctga atggctggca gaacatccgc aagtagcgcg agttaaccac cctgctctgc
     6661 ctggcagtaa aggacacgaa ttctggaaac gagactttac aggtagcagc gggctatttt
     6721 cctttgtgct taagaaaaaa ctcagtaatg aagagctggc gaactatctg gataacttca
     6781 gtttattcag catggcctac tcgtggggcg ggtatgaatc gttgatcctg gcgaatcaac
     6841 cagaacatat cgccgccatt cgcccacaag gcgagatcga ttttagcggg accttgattc
     6901 gcctgcatat tggtctggaa gatgtcgacg atctgattgc cgatctggac gccggttttg
     6961 cgcgaattgt ataacattgc cacttttgga caattttgca gacatttaat tgtgaaaagt
     7021 cttaaattgt tgcgtccggg atcaaggcgt cccggacgaa tcaggagtac aataggtaga
     7081 taaaggctta aacgctgttc cacaggaaag tccatggctg ttattcaaga tatcatcgct
     7141 gcgctctggc aacacgactt tgccgcgctg gcggatcctc atattgttag cgttgtttac
     7201 tttgtcatgt ttgccacgct gtttttagaa aacggcctgc tgcccgcctc atttttgcca
     7261 ggcgacagct tgttgatact ggcaggcgca ttgattgccc aaggggttat ggattttctg
     7321 cctacgattg cgattctgac cgccgcagca agtctgggct gctggctaag ttatattcag
     7381 gggcgctggt tagggaatac caaaacggtg aaaggctggc tggcacagct tcctgctaaa
     7441 tatcaccagc gcgccacctg catgtttgac cgccacggtc tgctggcgct gctggctgga
     7501 cgttttcttg catttgtccg tacgctgctg ccaaccatgg cgggaatttc cggtctgcca
     7561 aaccgccgct tccagttttt caactggtta agtggattgc tgtgggtcag cgtggtaacc
     7621 agttttggct atgccttaag tatgattccg ttcgttaaac gccatgaaga tcaggtaatg
     7681 acgttcctga tgatcctgcc aattgccttg ttaaccgctg gcttgttagg cacgctgttt
     7741 gtggtgatta aaaaaaaata ctgtaacgcc tgacgatttt ccccgttccc ggctgctgta
     7801 ccgggaacgt atttaattcc cctgcatcgc ccgcattctt gccgcatctt cccccggcgt
     7861 cacaccgaag taacgtttaa actcacggct aaattgcgat gcgctttcat agccgacgcg
     7921 catcgctgct gcgctggcct tcatgccgtc atggatgatc atcatccgcg ccttatgcag
     7981 acggtaattc ttcaaatact gcaacggcga ggtgctggtg acagacttaa aattatggtg
     8041 gaacgccgat acgctcatgt tggcttctgc cgccagttgc tcgacgctca ggttttcggt
     8101 gtatttattc tcaatccgtt tcagcacgcg gctaatcaga ctgaagtgag tctggcgact
     8161 gaccagcgcc agtaacgcgc cgccgcaagg tccggtcagc acgtagtaca gaatttcgcg
     8221 gatgatctgt ttgccgagaa tacgcgcatc cagtggtcgc tccatcacgt cgagtaaccg
     8281 ctccgccgcg cataaaatct cttctgataa cgtggcggag ttaatcccgc tggctgccat
     8341 cgacggctgg aaatgctcat cttcgccaat gtccatcaac agttcctgta actgcaaaat
     8401 atcgacattg agacgcaacc ctgccagcgg cacctctgac gtggcatagg tttcgcactc
     8461 aaacggcaac ggcaccgtca gcagcaggta ttcattggca tcataacgaa acacgcgttc
     8521 attgatataa ccgattttat gcccggaaaa gagaattatg atgccaggct cgtacatcac
     8581 cggtgtacgt gcgaaaggcg tctcgccata caacaaacgc acatcgggca acagttctga
     8641 caaactattt tctttaattt tcagtttatt aactttatcc gccagcaagc ggcaaatctc
     8701 ttcacgtttc atatcgcgta atttcttagg aataatgcgg caatttgatt gtgcgcaatt
     8761 ttgtagcatt tctccagcac tctggaggaa taggcaagac attggcagaa atgagcattg
     8821 agagccaggg cgctggcgat cacaatgaaa aacatcaggc agatcgttct ctgccctcat
     8881 attggcccag caaagggagc aagtaatgaa caactttaat ctgcacaccc caacccgcat
     8941 tctgtttggt aaaggcgcaa tcgctggttt acgcgaacaa attcctcacg atgctcgcgt
     9001 attgattacc tacggtggcg gcagcgtgaa aaaaaccggc gttctcgatc aagttctgga
     9061 tgccctgaaa ggcatggacg tgctggaatt tggcggtatt gagccaaacc cggcttatga
     9121 aacgctgatg aacgccgtga aactggttcg cgaacagaaa gtgactttcc tgctggcggt
     9181 tggcggcggt tctgtactgg acggcaccaa atttatcgcc gcagcggcta actatccgga
     9241 aaatatcgat ccgtggcaca ttctgcaaac gggcggtaaa gagattaaaa gcgccatccc
     9301 gatgggctgt gtgctgacgc tgccagcaac cggttcagaa tccaacgcag gcgcggtgat
     9361 ctcccgtaaa accacaggcg acaagcaggc gttccattct gcccatgttc agccggtatt
     9421 tgccgtgctc gatccggttt atacctacac cctgccgccg cgtcaggtgg ctaacggcgt
     9481 agtggacgcc tttgtacaca ccgtggaaca gtatgttacc aaaccggttg atgccaaaat
     9541 tcaggaccgt ttcgcagaag gcattttgct gacgctgatc gaagatggtc cgaaagccct
     9601 gaaagagcca gaaaactacg atgtgcgcgc caacgtcatg tggggggcga cgcaggcgct
     9661 gaacggtttg attggcgctg gcgtaccgca ggactgggca acgcatatgc tgggccacga
     9721 actgactgcg atgcacggtc tggatcacgc gcaaacactg gctatcgtcc tgcctgcact
     9781 gtggaatgaa aaacgcgaga ccaagcgcgc taagctgctg caatatgctg aacgcgtctg
     9841 gaacatcact gaaggttccg atgatgagcg tattgacgcc gcgattgccg caacccgcaa
     9901 tttctttgag caattaggcg tgccgaccca cctctccgac tacggtctgg acggcagctc
     9961 catcccggct ttgctgaaaa aactggaaga gcacggcatg acccaactgg gcgaaaatca
    10021 tgacattacg ttggatgtca gccgccgtat atacgaagcc gcccgctaag ctttttacgc
    10081 ctcaaacttt cgttttcggg catttcgtcc agacttaagt tcacaacacc tcaccggagc
    10141 ctgctccggt gagttcatat aaaggaggaa cgtatggcta atccaaccgt tattaagcta
    10201 caggatggca atgtcatgcc ccagctggga ctgggcgtct ggcaagcaag taatgaggaa
    10261 gtaatcaccg ccattcaaaa agcgttagaa gtgggttatc gctcgattga taccgccgcg
    10321 gcctacaaga acgaagaagg tgtcggcaaa gccctgaaaa atgcctcagt caacagagaa
    10381 gaactgttca tcaccactaa gctgtggaac gacgaccaca agcgcccccg cgaagccctg
    10441 ctcgacagcc tgaaaaaact ccagcttgat tatatcgacc tctacttaat gcactggccc
    10501 gttcccgcta tcgaccatta tgtcgaagca tggaaaggca tgatcgaatt gcaaaaagag
    10561 ggattaatca aaagcatcgg cgtgtgcaac ttccagatcc atcacctgca acgcctgatt
    10621 gatgaaactg gcgtgacgcc tgtgataaac cagatcgaac ttcatccgct gatgcaacaa
    10681 cgccagctac acgcctggaa cgcgacacac aaaatccaga ccgaatcctg gagcccatta
    10741 gcgcaaggag ggaaaggcgt tttcgatcag aaagtcattc gcgatctggc agataaatac
    10801 ggcaaaaccc cggcgcagat tgttatccgc tggcatctgg atagcggcct ggtggtgatc
    10861 ccgaaatcgg tcacaccttc acgtattgcc gaaaactttg atgtctggga tttccgtctc
    10921 gacaaagacg aactcggcga aattgcaaaa ctcgatcagg gcaagcgtct cggtcccgat
    10981 cctgaccagt tcggcggcta acatgcaaat tctcccggtg gcggtaatgt tccgctaccg
    11041 gacttttcag aaatcattta ttcccctcgc gtcccgcccg ttgttactct tccttgttca
    11101 ggaatgccaa atataaggac atcatcatgc agagccggaa gctcttaaaa gaacaactca
    11161 tctatatccg ggataaacgc aacggagagg tgaaaaacag atgaaaataa tacttctgtt
    11221 tttagcagcc ctggcaagtt ttaccgtaca cgcacagccc ccctcactga ccgtagaaca
    11281 aacagtccgg catatttatc agaactataa atcagatgcc actgcccctt attttggtga
    11341 aaccggagag cgggcgataa cttctgcgcg tattcaacag gcgcttaccc tgaacgacaa
    11401 tcttacgctg ccgggcaata ttggctggct ggattatgat ccggtttgtg attgtcagga
    11461 ttttggcgat ctggtgctag aaagcgttgc gataacccaa actgacgccg atcatgccga
    11521 tgccgttgtg cgctttcgta tctttaaaga tgataaagaa aagaccacgc agacactgaa
    11581 aatggtggcg gaaaatggtc gttgggtcat tgacgatatt gtcagcaatc atggcagcgt
    11641 cttacaagca gttaatagcg agaatgaaaa aacgctggcc gctttagctt cgttgcaaaa
    11701 agaacagccg gaagcctttg ttgccgaact ctttgaacat attgctgatt atagctggcc
    11761 gtggacgtgg gtggtttccg actcttaccg ccaggcggtt aatgccttct ataaaaccac
    11821 cttcaagacg gccaataatc ccgatgaaga tatgcaaata gaacggcaat ttatttacga
    11881 caatccgatc tgttttggcg aagagtcgct attttcacgc gttgatgaaa ttcgagtcct
    11941 ggagaaaacc gccgattccg cccgcattca tgttcgtttt acgctgacca atggcaacaa
    12001 cgaagagcaa gaactggttt tacagcggcg cgaaggcaag tgggaaatcg ctgattttat
    12061 ccgcccgaac agcggcagcc tacttaagca gattgaggca aaaaccgccg ccagattaaa
    12121 gcaatgagct gaattaaata acaattagcc ggaacaataa ataaaaggga acactatatg
    12181 aaaacgattt tcaccgtggg agctgttgtt ctggcaacct gcttgctcag tggctgcgtc
    12241 aatgagcaaa aggtcaatca gctggcgagc aatgtgcaaa cattaaatgc caaaatcgcc
    12301 cggcttgagc aggatatgaa agcactacgc ccacaaatct atgctgccaa atccgaagct
    12361 aacagagcca atacgcgtct tgatgctcag gactattttg attgcctgcg ctgcttgcgt
    12421 atgtacgcag aatgataaaa aaatccccgg cagcatgtca gttgccgggg atttttttta
    12481 acgtccaacc gccgctttag ggcgtttctt cgcaccagca ttcaccggac gagattgcgt
    12541 agacgacgct ttttttgccg tagcaggcgt ctgacgctgg gtcgccatcg gcgtatgttt
    12601 cgtcaacgcc ggacgggtat tgcggttctg gcgacgagct tcacgcatct cttcaatggt
    12661 tggcgcaggc actaagcaat cgcgacggct gccaatcaga tgctttttgc ccatcgcttc
    12721 cagcgcctgg cggattaacg gccagtttgc cggatcgtgg taacgcaaca acgctttatg
    12781 caaacgacgc tgcttgtcgc ccttcggtac gaagacgtct tcactcttat aaccaatctt
    12841 cgccagcggg tttttcccgg tgtaatacat ggtggttgag ttcgccagcg gcgatgggta
    12901 gaagttctgt acctggtcga gacggaaacg atgctttttc agccacagcg ccagattcac
    12961 catatcttca tcacgcgtac cggggtgcgc ggagatgaaa taggggatca gatactgctc
    13021 tttacctgcc tgttttgagt aagtatcgaa cagctcttta aagcggtcat agctgcccat
    13081 gcccggcttc atcatcttcg ataacggccc ttcttcggta tgttccgggg caatcttcag
    13141 ataaccgccg acgtgatggg tcgccaactc tttgatatag cgcggatctt ccacggcgat
    13201 gtcataacgt acgcctgagg cgatgaggat ttttttaatg cctttcagat cacgcgcacg
    13261 gcgatagagg ttgatcgtcg gttcgtggtt agtgtccatg tgcggacaaa tatccggata
    13321 aacgcacgac aaacggcgac aagtttgttc agcgcgtggc gatttgcagc gcaacatata
    13381 catgttggca gttggcccac cgagatcgga aatcacgccc gtaaaacctg gaacggtgtc
    13441 gcggatcgct tcgatctcat taatgatcga atcttccgaa cggctctgaa taatgcgccc
    13501 ttcgtgctcg gtaatagaac agaaagaaca gccgccaaag cagccgcgca taatgttgac
    13561 cgaaaaacgg atcatttcgt aagccggaat acgggcattg ccataggccg gatgtggcac
    13621 gcgcttgtac ggcagcgcaa aaacgctgtc catctcttcg gtagaaagcg ggatagcagg
    13681 cgggttgatc cacacatagc ggtcaccgtg tttttgcatc aatgcgcggg cgcagcctgg
    13741 gttggtttcg tggtgcagaa tgcgcgaagc atgggcgtac agcactttat cgcccttcac
    13801 tttctcgaaa gaaggcagca acacgtaggt tttttcccac ggtttcgggc gcggtggctg
    13861 cacggttacg gctttggctt cctgcttttt cggtgccacc ggtttgttat ccgcgcacgg
    13921 caaatcttca ccatacggat gcgggattgg gtcgattttt ccaggggtat caagacgggt
    13981 ggaatccacg ccgctccagc caggcagcgc ctctttcacg ataatcgcgg tattacgcac
    14041 atcgcggatt tcactaatcg gctcgcccat cgccagacgg tgcgccacct ccaccagcgg
    14101 acgctcaccg ttaccaaac
//
LOCUS       DAMZET010000110        13946 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.110, whole genome shotgun sequence.
ACCESSION   DAMZET010000110 DAMZET010000000
VERSION     DAMZET010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.110"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..665)
                     /locus_tag="POB34_003270"
     CDS             complement(<1..665)
                     /locus_tag="POB34_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HDI5920162.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKL"
     gene            complement(675..1121)
                     /locus_tag="POB34_003271"
     CDS             complement(675..1121)
                     /locus_tag="POB34_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDI5920163.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            1244..3298
                     /gene="helD"
                     /locus_tag="POB34_003272"
     CDS             1244..3298
                     /gene="helD"
                     /locus_tag="POB34_003272"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HDI5920164.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(3330..3788)
                     /gene="mgsA"
                     /locus_tag="POB34_003273"
     CDS             complement(3330..3788)
                     /gene="mgsA"
                     /locus_tag="POB34_003273"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDI5920165.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(3884..4546)
                     /locus_tag="POB34_003274"
     CDS             complement(3884..4546)
                     /locus_tag="POB34_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HDI5920166.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            4719..5132
                     /locus_tag="POB34_003275"
     CDS             4719..5132
                     /locus_tag="POB34_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDI5920167.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(5177..5494)
                     /gene="hspQ"
                     /locus_tag="POB34_003276"
     CDS             complement(5177..5494)
                     /gene="hspQ"
                     /locus_tag="POB34_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HDI5920168.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(5552..6742)
                     /gene="rlmI"
                     /locus_tag="POB34_003277"
     CDS             complement(5552..6742)
                     /gene="rlmI"
                     /locus_tag="POB34_003277"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HDI5920169.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            6837..7115
                     /gene="yccX"
                     /locus_tag="POB34_003278"
     CDS             6837..7115
                     /gene="yccX"
                     /locus_tag="POB34_003278"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDI5920170.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELADFRIR"
     gene            complement(7112..7441)
                     /gene="tusE"
                     /locus_tag="POB34_003279"
     CDS             complement(7112..7441)
                     /gene="tusE"
                     /locus_tag="POB34_003279"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HDI5920171.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(7532..8191)
                     /gene="yccA"
                     /locus_tag="POB34_003280"
     CDS             complement(7532..8191)
                     /gene="yccA"
                     /locus_tag="POB34_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HDI5920172.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(8398..8485)
                     /locus_tag="POB34_003281"
     tRNA            complement(8398..8485)
                     /locus_tag="POB34_003281"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8449..8451),aa:Ser,seq:tga)
     gene            8912..10030
                     /gene="hyaA"
                     /locus_tag="POB34_003282"
     CDS             8912..10030
                     /gene="hyaA"
                     /locus_tag="POB34_003282"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HDI5920173.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGNQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            10027..11820
                     /gene="hyaB"
                     /locus_tag="POB34_003283"
     CDS             10027..11820
                     /gene="hyaB"
                     /locus_tag="POB34_003283"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HDI5920174.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            11839..12546
                     /gene="hyaC"
                     /locus_tag="POB34_003284"
     CDS             11839..12546
                     /gene="hyaC"
                     /locus_tag="POB34_003284"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HDI5920175.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            12543..13130
                     /locus_tag="POB34_003285"
     CDS             12543..13130
                     /locus_tag="POB34_003285"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HDI5920176.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            13127..13525
                     /gene="hyaE"
                     /locus_tag="POB34_003286"
     CDS             13127..13525
                     /gene="hyaE"
                     /locus_tag="POB34_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HDI5920177.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            13522..>13946
                     /locus_tag="POB34_003287"
     CDS             13522..>13946
                     /locus_tag="POB34_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HDI5920178.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLA"
BASE COUNT         3449 a         3554 c         3770 g         3173 t
ORIGIN      
        1 agtttcgtct gattcaatgt cgccatcagc agaccgttgg cgagagccaa atcgtacagc
       61 ggtgcctggc tttcatcttc aatatcagga tcaaacatgc gcgacttgag ctcaagataa
      121 cgcgccagtt gttcatagca acgcgccagg ttgtcctgca gcgggcggac cgggaacagc
      181 agatgaccaa taagtgtcag gacgttgtac cagacggcac cggccagcag atacatcggc
      241 tgctgatacc agtgctcata cagtgatgtt cccaacatag tgtaaatggc gatcagcaat
      301 gcaccgaagg caattgttgc atagcgttga cccagaccgc cgagcaaaat gaagccgctg
      361 gtagagagcg ttaagccaat cgcaaatagc cagggccagg gaaacagcaa ttctactgag
      421 gccgaggcga taaaaaagca gaacagcgta atgatgaggt tacgcaaacg tcccgccagt
      481 cggtcatcga gatcggtcag cgccgctgcc accatcccca gcgttagcgg aatcgtcagt
      541 tttacatcac ccagccacca cggaaacgct gtggttccac aaagcgcaat aaaaatacgc
      601 gcgtaataca gccaggcgct gttccaggta tagcgtttga gcaaaggact tagcataaag
      661 gccatattag tggattattc aaaacgacga cgtgcattgg cttcacgcgc agcttgcgct
      721 gtttctaccg ataccacgcg acgaccaacc ggccatagtg caatagcggc aattttaaag
      781 ttcgcaatgc cgacaggaat gccaatgatt gaaatacatt gtgcgatgcc cgttgcaatg
      841 tgcatcaggc ataaccacca gccaaagaaa atcagccaga aaatattcaa taccgtaccg
      901 ccagtattca gcagcacatt tttgccagcc gggttcagtt catcgacatg aatagcttca
      961 ttgccataag gcaccagaga cagtttagtg atctcccagc aggatcgtgt cagcggtaag
     1021 gtaaaaatca gcacaatact gaccagagtc gccaacagcc agcccagagt ggtggcaaat
     1081 ccgccaagca caaagttcag aatgttcaaa acggtacgca taaaacctcg ctttactgtg
     1141 gttttcagta atgggcggca attgtaacgt ttttttgggc tggagcacgt tttctctgac
     1201 ggttacactg ataagaaata atttcgtgtg gatctacaga gtcatggaac tgaaagcgac
     1261 aacgcttgga aaacgtctgg cacagcaccc ttacgatcgg gcggtgatcc tcaatgccgg
     1321 gattaaagtc tccggcgatc gccacgaata ccttattcct ttcaatcaat tactggcgat
     1381 tcactgtaag cgcggtctgg tatggggcga gctggaattt gtactgccgg acgaaaaagt
     1441 ggtgcgtctg cacggcaccg aatggggcga gacgcagcgt ttttaccatc atcttgatgc
     1501 tcactggcgg cggtggagtg gcgagatgag cgaaattgcg tctggtgttt tacgccagca
     1561 actggatttg attgccacgc gcactggaga aaataaatgg ctgacgcgtg agcaaacctc
     1621 tggcgttcag caacaaatcc gccaggcttt gtcggcgttg ccgttgccgg ttaaccgact
     1681 ggaagaattc gataactgcc gtgaggcgtg gcgtaaatgt caggcctggt tgaaagatat
     1741 tgaaagcgct cggttgcagc ataaccaggc gtataccgaa gccatgctta ccgagtatgc
     1801 ggattttttc cgccaggtcg agtcttcacc gctgaatccg gcgcaggccc gggcagttgt
     1861 taatggcgag cattctctgt tagtgctggc aggtgcagga agcggaaaaa cgtcggtgct
     1921 ggtggcccgt gcaggctggt tgctggcgcg tggtgaagcg tcccctgagc aaattttatt
     1981 gctggcgttt ggtcgcaaag ccgctgaaga gatggacgag cgtattcgcg aacggctaca
     2041 taccgaagac attaccgcac gcacgtttca tgcgctggcg ctgcatatta ttcagcaggg
     2101 cagcaaaaaa gttccgatag tcagcaaact ggaaaatgat accgctgccc gtcatgaact
     2161 ctttattgct gagtggcgca agcaatgcag cgaaaagaaa gcgcaggcga agggctggcg
     2221 gcaatggctg acggaagaaa tgcagtggtc agtgccagaa ggtaacttct gggatgatga
     2281 aaaattacag cgtcgtcttg cctcacgcct cgatcgttgg gtaagtctga tgcggatgca
     2341 cggtggtgca caggcagaaa tgattgccag tgcacccgaa gagattcgcg atctgttcag
     2401 taaacgtatc aagttgatgg ccccgttatt aaaagcctgg aaaggtgcgc tgaaggcaga
     2461 aaacgctgtc gatttttcgg gccttattca tcaggcgatt gtgattctgg agaaaggccg
     2521 ctttatcagc ccgtggaagc atattctggt tgatgaattt caggatatct cgccgcagcg
     2581 cgcagcgttg ttagcggcat tacgcaagca aaacagtcag acgacgttgt tcgccgttgg
     2641 tgatgactgg caggcgattt accgattcag cggtgcgcaa atgtcgctca ccaccgcttt
     2701 ccatgaaaac tttggtgaag gcgatcgctg tgatttagac acgacttacc gttttaacag
     2761 tcgtatcggt gaggtggcaa accggtttat tcagcagaac ccaggccagc tgaaaaagcc
     2821 gctaaacagc ttaaccaatg gagacaaaaa agccgtcacg ttattggatg agagtcaact
     2881 tgacgctttg ctggataagc tctctggtta tgccaaaccg gaagagcgca ttctgatcct
     2941 ggcgcgttac catcacatga ggcctgccag cctggaaaaa gcggcaacac gctggccgaa
     3001 gttgcaaatc gactttatga ccattcatgc cagcaaaggg caacaggcgg attacgtcat
     3061 catcgttggc ttgcaggagg ggagtgatgg ttttccggct gcggcgcggg agtcgattat
     3121 ggaagaggcg ctactgccac cggttgagga tttcccggac gctgaagaac ggcggttaat
     3181 gtacgtggcg ctgacccggg cacgccatcg ggtatgggca ctgtttaaca aagagaatcc
     3241 ctctcccttt gtggaaatac tgaaaaatct ggatgtgccg gtagcgagaa aaccgtaaga
     3301 aacaggtggc gtttgccacc tgtgcaatat tacttcagac ggtccgcgag ataacgctga
     3361 taatcgggga tcagaatatc gaccgcgtcg ttgaaatgcg gcgactggat tatgaagtct
     3421 gccgttgcca cgttggtggc gaccggaatg ttccataccg tcgccagacg caacaaggct
     3481 ttcacgtcag gatcgtgcgg cacggcattt agtgggtccc agaagaaaat caatacatca
     3541 attttccctt ctgagatcaa tgcgccaacc tgctggtcac cccccattgg gccactcaac
     3601 atcgcgttga cgttcatgcc ggtcgcgcgg gaaattaagt taccggtagt gcctgttgca
     3661 tacagtacgt gttgttccag taacggttga tgccgttcca cccagctcat cagcatttgt
     3721 ttgcagtgat cgtgtgccac cagcgcaata tgtttccgcg caggtaaagt gcgagtcgtc
     3781 agttccataa tgtacatccg tagttaactt tcctacagat tactgtaagc acttatcgct
     3841 gcaagataaa gaccgaaaaa gcctgcgcac aggcacaaaa atctcaggaa gatggttgtt
     3901 tttccgccca ttgtaggaaa gtatttcgcg tttgtgggtc agccagttta aaccaatact
     3961 tcagccgttg ttctgtgagc acctgagact gcggtggaat agcatccagc tcggaaacac
     4021 ccgaaagcag cgtactgtca tctgccatca tcgtggcaaa ttgcggtagt gaagcgcgtt
     4081 tcgcggattt gttatagcgt tcaacctcga cctcataatc aatcgttttt gccgttgagg
     4141 taatggcgag aatatccagc tttaccggaa tcggcgtcgc atcgccatcc aacaattcaa
     4201 ggcgcggcgc ggcatcaaaa tggttagctt cccgctcatt ctccaggcga ggcaggcgaa
     4261 aattgacctg gttgatgagc tgggtattaa aactgaccac cagcggtggg gagatataca
     4321 gccgttcttc actattggaa agatgaatcg tcttctcaac gcgaaacact aactgatgcg
     4381 gtccattatc cagttcaatg ctgtccgcac cgcgcagcag agagctggaa accttcttgc
     4441 cgtcgagtac cagaagatcg acatcggttg aaagccgcag cgtggtggca aaaacggata
     4501 ccggcaaaca taatgcaatc aaggtggtca cgatgccggt tttcataggt agctcctgtc
     4561 aaaagaccgc ccgaataata atgtgtcaaa atttttcttt aatcatactt actaacatat
     4621 agacatattt cccgcttttg ctctcaatca ttcgtattag ctgcatggtt ggcatgtcgg
     4681 ctttgtcgta cactttgtta aacagtctgg aggaaataat gaaagaaacc gatattgcag
     4741 gtattttaac gtccactcac accattgcgc tggtgggggc aagcgacaaa cccgatcgtc
     4801 ccagctatcg cgtgatgaaa tatctactcg accaggggta tcacgtcatt ccggtttcgc
     4861 caaaagttgc cggcaaaacg ctgctgggcc agcaggggta tggcacgctg gcagacgttc
     4921 cggaaaaagt cgatatggtc gatgttttcc gtaattcaga agcggcgtgg ggcgtggcgc
     4981 aggaagctat cgccattggc gcgaaaaccc tctggatgca actgggggtg attaatgagc
     5041 aagcggcagt actggcacgg gatgccgggt taaatgtggt gatggatcgc tgcccggcta
     5101 ttgagatccc tcgcctgggc ctggccaaat aaaaaatccc cggaaggcaa aaaccttccg
     5161 gggattcgtt cagggatcag ttacgcagac gcggggcctg gagttgtttg cggatggtct
     5221 gcgccagctc atccatggat ggctgttcag gatgttcatc ctgcaattcg ctacttagct
     5281 gggcttcagc caggtaggta tgaaccggta ggccgttatc gtcctccatc accacgtgat
     5341 accacggagc ggcgcgaagc tcgtcattca ccgccagctc atcaggcgac ggttcagaga
     5401 gcgaataaac cgggtcgata tccacgacca ctccgagata acctaacagg gaatggcgga
     5461 cctgctggcc gataccgaat ttgctggcaa tcatagtcac ctcccgggaa atctttacct
     5521 ttgatataag ggtaaaattc cacatttcaa gttacatgac gcgacaggca aaccctttca
     5581 gatatagccc ttccggatag gtagcgatca ccggatgatc ggctgcctga cggaactgct
     5641 ctataaattg tacatcacgg ccggcatcaa ttgcggcatc cgcgatgatt ttctgaaata
     5701 aatcgctggt catcagaccg gaacaggaga aagtcaggag aataccgcct tcattcagca
     5761 actgaatcgc cagcatgttg atatctttat agccacgaca cgcgcccatc aactggcttt
     5821 tattctcaac aaacttcggc gggtccatca cgataacgtc aaatttttca ccgcgatcgc
     5881 gataagtacg cagcaattta aagacatcat cacggacaaa ctcagccttg ctcagatcca
     5941 gtttgttcag ctcaacgttc tgccgtgcaa tatccagcgc ttcctgggag gtatcaacgc
     6001 tgacaacctg gctgcaaccg cccatcagtg ccgatacggc gaaaccaccg gtataggaga
     6061 aacagttcag cacacgttta ttttcaacgt agcggcgggt agccaggcgg ctgtctcgct
     6121 ggtccaggta gtagcccgtt ttgtgtccgt gctgaatatc caccagcagt ttcatgccgt
     6181 gttcttcaat cggcagcagg gcaggtggca actcgccggt gacgaggccc tgggtcagct
     6241 ccatcccttc ttttttacgt accgcaacat cgctgcgatc gtaaatcgca cattccgggt
     6301 acagcgtttg cagggcacta attaatgccg cgcgctgata ttctgcgcca gcactcagca
     6361 gttgcagcac cagaaaatta ccgaaacgat cgatagtaat acccggcagg ccatcagatt
     6421 ctccggcgat taaacgatag ctgtcgaggc catctttttg cgccagccag tcacgccatt
     6481 tttgtgcttg ttgcaaacgg cgggaaaaaa aagcaatgtc gatagactca gacgggtcaa
     6541 acgtccagac gcgcgcccgg atttgcgaag ctggcgaata agcgccgcgt gctaaccatt
     6601 ttccctgatg atcaacaata tcgatggttt caccgaggct ggctttacct tccatgcggg
     6661 caacggcccc ggaaaagacc cacggatggc gacgaagtaa tgatttttcg cgccctttgg
     6721 ctaacactaa acgtacactc ataatttact tttctgtcga tgcaaagaaa tgggcgtcat
     6781 tgtccggagt tttccatgga tttgcaacgc gtccattaag gataaggaga gcagcaatgt
     6841 cgaaagtctg cataattgcc tgggtttacg ggcgagttca gggcgtagga tttcgctaca
     6901 ccacacagta tgaagcgaaa agactgggtt taaccgggta cgccaaaaat cttgacgatg
     6961 gcagcgtgga agtggttgcc tgcggtgaag aagggcaggt ggaaaaatta atgcagtggt
     7021 taaagagtgg cggcccgcgt tctgcgcgtg tggaacgggt gcttagcgag ccgcatcatc
     7081 cctcggggga attagccgat tttcgtatcc gttaaataca ttttaccggt tttggcaggc
     7141 cagcaatttt ggtggcttgc ttagccggac ctttcggaaa cagtcgatat aaatagcggc
     7201 tgttaccttt ttcttcgcca aatttattcg ccatcgcttt taccagcata cgaatcgccg
     7261 gagaagtatt gaattccaga tagaaatcac gcacaaaacg caccacttcc cagtgttctg
     7321 gcgacagcga aatcccttcg ttctctgcaa tcaccacagc cagcggctca ctccactggc
     7381 tgctttcttt gagatagcct tcggtatccg tttctatctc tttaccttcg aagatcagca
     7441 taattattac taccttaatc agactggcgg cagtgtaaca aaaaacaaag ccccgcataa
     7501 agcgaggcta tgaaagtgtt agcgggtgag attaatcgcg gctagcgaag cccagaatgc
     7561 tcagcaggct gacgaagatg ttgtacagcg aaacatacag gctaaccgtg gcacgaatat
     7621 agttcgtctc accgccatga atgatgttgc tggtttcaaa caagatagcg ccagaggaga
     7681 tcagaatgaa gaccgcgctg atcgccagat gcagagcagg cagttgcagg aagatattag
     7741 caaccatacc aatcagcacc accacaatac ccgccatcag cataccgccg aggaacgaca
     7801 tatctttgcg ggtggtcagc acatatgcag agcagcagaa gaacactaac gccgttccgc
     7861 ccagtgccat agcgattacg tcacccattc cggcagacag ataggtgttc agaataggtc
     7921 cgaggatata acccagaaaa ccggtaaagg cgaatgcgga gataatcccg gtcggcttat
     7981 tcgccgtttt ataggtcagg aacatcaaac catacatacc caccagcgtc agaatcagac
     8041 ccggagatgg cagcatcagc accgtactgg cagttgcggt aatcgccgaa aaggccagcg
     8101 tcaggctcag cagaaaatag gtattacgca gcaccttatg ggtgctaagc agtgatgtac
     8161 ggtcatgtga agaactaaca atacgatcca ttgagtcact ctctatgaca gatgtaatta
     8221 attaagcagc ataatgataa tgcgtaaggg cacccagaag ttttacccat ctttacgcat
     8281 ttgatctgga acaggtttaa cagcggatta tcaggtcatt aagcaaatat aacgccctga
     8341 gaatttcgac aggcaaaaga aaaaggggtt agcatttagc taacccctta tcttatttgg
     8401 cggaagcgca gagattcgaa ctctggaacc ctttcgggtc gccggttttc aagaccggtg
     8461 ccttcaaccg ctcggccaca cttccggaat gacgcgcact ataaacatcc cgatgcggcg
     8521 tgtaaacccc taatttgttt gtttgcctga aaaacagcca aaagtgcatt gatagcgtga
     8581 aataacagca gattgatcat ttcatcacca tgaattcctt ctcttttact cgtttagcaa
     8641 ccggctaaac atccccaccg cccggccaaa agaaaaatag gtccattttt atcgctaaaa
     8701 gataaatcca cacagtttgt attgttttgt gcaaaagttt cactacgctt tattaacaat
     8761 actttctggc gacgtgtgcc agtgcagaag gatgagcttt cgttttcagc atctcacgtg
     8821 aagcgatggt ttgtcttgct acagggacgt cgcttgccga ccataagcgc ccggtgtcct
     8881 gccggtgttg ctaggagaag agacgtgcga tatgaataac gaggaaacat tttaccaggc
     8941 catgcggcgt cagggcgtta cccggcgcag ctttctcaaa tattgtagtc tggctgccac
     9001 gtcgctggga ttaggcgcgg gaatggcacc aaagattgcc tgggcgctgg agaacaaacc
     9061 gcgcattccg gtggtatgga tccacggtct ggaatgcacc tgctgtaccg aatcttttat
     9121 ccgctccgct cacccactgg cgaaggacgt catcctttcc cttatttctc tcgattacga
     9181 cgatactttg atggctgccg ccggaaacca ggcggaagaa gtctttgaag acatcatcac
     9241 gcaatacaat ggcaaatata tcctcgcagt agaaggcaat ccgccgctgg gcgagcaggg
     9301 gatgttctgt atcagcagcg gtcgaccgtt tattgagaaa ctcaaacgtg ccgctgccgg
     9361 agccagcgcg attatcgcct ggggaacctg cgcgtcctgg ggctgcgtgc aggccgcgcg
     9421 gcccaatccg acgcaggcaa cgcctatcga caaagtcatc accgacaaac ccattatcaa
     9481 agtacctggc tgcccgccga tcccggatgt gatgagcgcc atcattactt acatggtgac
     9541 ctttgatcgc ttgccagatg tcgacagaat gggccgtcca ctgatgtttt atggtcagcg
     9601 tatccatgat aaatgctatc gccgcgctca cttcgacgcc ggagagttcg tccagagttg
     9661 ggatgatgac gctgcccgca aaggttactg cctgtacaaa atgggctgca aagggcctac
     9721 cacctataac gcctgttcct ccacacgctg gaatgatggc gtttcttttc caatccagtc
     9781 tggtcacggc tgcctgggct gtgcggaaaa tggtttctgg gatcgcggtt cgttctacag
     9841 ccgcgtggtc gatattccgc aaatgggtac tcattccact gccgataccg tcggtttaac
     9901 cgcgcttggc gtggtggcag cggctgttgg tgtgcacgca gtcgccagcg ccgttgacca
     9961 gcgcagacgt cataaccagc aacctacaga aaccgaacat cagccaggca atgaggataa
    10021 acaggcatga gcactcagta cgaaactcag ggatacacca tcaataatgc cggacgccgc
    10081 ctggtggtcg acccgattac gcgcatcgaa ggccacatgc gctgcgaagt gaatattaac
    10141 gatcagaatg tgatcaccaa tgccgtctcc tgcggcacca tgtttcgcgg gctggagatc
    10201 attctgcaag ggcgcgaccc gcgcgatgcg tgggcgttcg ttgaacgtat ctgcggcgtc
    10261 tgtactggcg tacacgccct ggcttcggtt tacgccatcg aagatgctat cggtattaaa
    10321 gtgccggaca acgccaatat catccgcaac attatgctgg caacgctctg gtgccacgat
    10381 catctggtgc acttctatca gcttgccggg atggactgga tcgatgtgtt agatgcgctg
    10441 aaagccgacc cgcggaaaac ctccgaactg gcgcaaagtc tctcctcttg gccgaaatca
    10501 tcccctggct atttcttcga cgtacaaaac cgcctgaaaa aatttgttga aggcgggcag
    10561 ttggggatct tccgcaatgg ctactggggg cacccgcagt acaaactgcc gccagaagct
    10621 aacctgatgg gctttgccca ctatctcgaa gctctcgatt tccagcgtga aattgtcaaa
    10681 atccacgcgg tctttggcgg taaaaacccg catccaaact ggattgtcgg cgggatgcct
    10741 tgcgccatca acattgacga aagcggcgcg gtcggggcag tcaatatgga acgcctgaac
    10801 ctggtgcagt caattatcac ccgcacggcg gacttcatta acaacgtgat gatccccgac
    10861 gccttagcca tcggtcagtt caacaaaccg tggagcgaaa tcggcacggg tctttctgat
    10921 aaatgtgtcc tcagctacgg ggcattcccg gatattgcca acgactttgg tgagaaaagt
    10981 ctgctgatgc ctggcggcgc ggtgattaac ggcgatttca acaatgtgct gccagtggat
    11041 ttggttgatc cgcagcaggt gcaggagttt gtcgatcacg cctggtatcg ttatcccaac
    11101 gatcaggtcg ggcgtcatcc gtttgatggc atcaccgacc cgtggtacaa ccccggcgat
    11161 gtcaaaggca gcgataccaa cattcagcag ctgaatgaac aggaacgcta ctcgtggatc
    11221 aaagcgccac gctggcgcgg taacgcgatg gaagtggggc cgctggcgcg cacgttaatc
    11281 gcttatcaca aaggcgatgc tgcgaccgtt gagtcggtcg atcgcatgat gtcggcgttg
    11341 aacctgccgc tttccggtat ccagtcaacg ttaggccgca ttttgtgccg cgcgcacgaa
    11401 gcgcagtggg ccgcaggtaa gttgcagtat ttcttcgaca agctgatgac caacctgaaa
    11461 aacggcaatc tcgccactgc ttccacggaa aaatgggaac ctgcaacctg gccgacagag
    11521 tgccgtggtg tcggttttac cgaagcgccg cgcggggcgt taggccactg ggccgccatt
    11581 cgcgatggca agattgatct ctaccagtgc gtggtgccga ccacctggaa cgccagcccg
    11641 cgcgatccca aagggcagat tggcgcttat gaagcggcgc tgatgaacac caaaatggcg
    11701 atccccgagc aaccgctgga gatcctgcgt actctgcaca gctttgaccc gtgcctcgcc
    11761 tgttcaacac acgtgctggg cgacgacggt agcgagctga tctccgtgca ggtgcgttaa
    11821 cagcgaagga gaatcatcat gcaacagaaa agcgacaacg ttgtcagcca ctatgtcttt
    11881 gaagcgccag tccgcatctg gcactggttg acggtgttat gcatggcggt gttgatggtc
    11941 accggatact ttatcggcaa gccgctacct tccgtcagcg gcgaggcgac gtatctgttc
    12001 tatatgggct acatcaggtt aattcacttc agcgccggga tggtttttac cgtggttttg
    12061 ctgatgcgga tctactgggc ttttgttggc aatcgatact cccgcgagct gtttatcgtg
    12121 ccggtatggc gtaaaagctg gtggcagggc gtgtggtatg aaatccgctg gtatctgttt
    12181 ctggcaaaac gtccgagtgc cgatataggc cataatccca tcgcccaggc ggcgatgttc
    12241 ggctatttcc tgatgtcggt ctttatgatc atcactggtt ttgcgctgta cagcgaacac
    12301 agccagtacg ctatttttgc gccgttccgt tatgtggtgg aatttttcta ctggacgggt
    12361 ggcaactcaa tggacattca cagctggcat cggctgggga tgtggctgat tggcgcgttt
    12421 gtgatcggtc atgtctacat ggcgctgcgt gaagacatca tgtccgacga cacggtgatc
    12481 tccaccatgg tcaacggcta ccgtagccac aaatttggca aaataagtaa caaggagcgt
    12541 tcatgagcga gcaacgcgtg gtggtcatgg ggctgggcaa cctgctgtgg gccgatgaag
    12601 gcttcggcgt gcgggtggcg gaacggctgt atgcccatta ccactggccc gagtatgtgg
    12661 agattgtcga tggcggtact cagggactga acttgctggg gtatgtcgaa agcgccagcc
    12721 atctgttgat tctcgatgcc attgactacg ggctggaacc tggaacgctg cgaacctatg
    12781 ccggagaacg cattccggct tatctcagcg cgaagaaaat gagcctgcat cagaacagtt
    12841 tctccgaagt gttggcgctg gcggatatcc gcggacatct gccagcacat attgccctcg
    12901 tcggtctgca acccgcaatg ctcgacgact acggcggtag cctgagcgaa ctggcacggg
    12961 agcaactgcc cgctgcggaa caggcggcgc tggcgcagct ggctgcgtgg ggaattgtgc
    13021 cgcaaccggc taatgaatcg cgctgtctca attatgactg tctgtcgatg gaaaattacg
    13081 aaggcgttcg cttgcgccag taccggatga cacaggagga gcagggatga gcaacgacac
    13141 gccatttgat gcgttgtggc aacgaatgct ggcgcgcggc tggacgccag tcagtgaatc
    13201 ccgtcttgac gactggctta cgcaagcgcc agacggcgtg gtgttattaa gcagtgaccc
    13261 gaaacgcacg ccagaggtca gcgataatcc ggtaatgatt ggcgaattac tgcacgagtt
    13321 tcccgactat acatggcagg tggcgattgc tgaccttgag cagagcgaag ccatcggcga
    13381 tcgctttggc gtctttcgct ttcctgccac tttagtgttt accggcggaa actatcgcgg
    13441 cgtgctgaat ggtattcacc cgtgggcgga actgataaac ctgatgcgcg ggcttgtcga
    13501 accgcagcag gagcgtgcct catgagcgaa acttttttcc atctgctggg gccaggaacg
    13561 caaccgaacg atgacagttt cagcatgaat ccactgccga tcacctgtca ggtgaatgat
    13621 gaaccgagta tggcggccct ggagcaatgt gctcacagcc cgcaggtgat tgcgctgtta
    13681 aacgagttac aacatcaact aagcgaacgc caaccgccgt tgggcgaggt gctggcagtc
    13741 gatctgttaa atctcaacgc cgacgatcgt cactttatca atacgcttct cggggaaggg
    13801 gaagtgtcag tgcgcattca gcaggctgac gacagtgaaa gtgaaataca ggaggcgatc
    13861 ttctgcggat tatggcgggt gcgcagacgt cgcggcgaaa agttgctgga ggacaaactg
    13921 gaggctggct gcgcgccgct ggcgtt
//
LOCUS       DAMZET010000111        13791 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.111, whole genome shotgun sequence.
ACCESSION   DAMZET010000111 DAMZET010000000
VERSION     DAMZET010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.111"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..666)
                     /locus_tag="POB34_003288"
     CDS             complement(<1..666)
                     /locus_tag="POB34_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HDI5920179.1"
                     /translation="MKSILIEKPNQLAIVEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGDGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIF"
     gene            complement(678..1892)
                     /gene="rspA"
                     /locus_tag="POB34_003289"
     CDS             complement(678..1892)
                     /gene="rspA"
                     /locus_tag="POB34_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HDI5920180.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(2098..2424)
                     /locus_tag="POB34_003290"
     CDS             complement(2098..2424)
                     /locus_tag="POB34_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HDI5920181.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            2559..2900
                     /locus_tag="POB34_003291"
     CDS             2559..2900
                     /locus_tag="POB34_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HDI5920182.1"
                     /translation="MKITLSKRIGLLAILLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2935..3495
                     /gene="speG"
                     /locus_tag="POB34_003292"
     CDS             2935..3495
                     /gene="speG"
                     /locus_tag="POB34_003292"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HDI5920183.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(3498..4208)
                     /locus_tag="POB34_003293"
     CDS             complement(3498..4208)
                     /locus_tag="POB34_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HDI5920184.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            4316..4621
                     /locus_tag="POB34_003294"
     CDS             4316..4621
                     /locus_tag="POB34_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HDI5920185.1"
                     /translation="MKLSTCCAALLLALASPVVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4820..7246
                     /locus_tag="POB34_003295"
     CDS             4820..7246
                     /locus_tag="POB34_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HDI5920186.1"
                     /translation="MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAATV
                     QQASEKVIWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGTRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     RTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            7307..9730
                     /locus_tag="POB34_003296"
     CDS             7307..9730
                     /locus_tag="POB34_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HDI5920187.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGKEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            9741..10358
                     /gene="dmsB"
                     /locus_tag="POB34_003297"
     CDS             9741..10358
                     /gene="dmsB"
                     /locus_tag="POB34_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDI5920188.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            10360..11214
                     /locus_tag="POB34_003298"
     CDS             10360..11214
                     /locus_tag="POB34_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HDI5920189.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLACIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWVCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            11257..11871
                     /gene="dmsD"
                     /locus_tag="POB34_003299"
     CDS             11257..11871
                     /gene="dmsD"
                     /locus_tag="POB34_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HDI5920190.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            12030..13322
                     /gene="clcB"
                     /locus_tag="POB34_003300"
     CDS             12030..13322
                     /gene="clcB"
                     /locus_tag="POB34_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HDI5920191.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFHHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYELIISTGVLAGLCGPLLLTLMNACHRGFVGLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(13275..>13791)
                     /gene="bioD"
                     /locus_tag="POB34_003301"
     CDS             complement(13275..>13791)
                     /gene="bioD"
                     /locus_tag="POB34_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDI5920192.1"
                     /translation="SVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANLTE
                     KVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIAND
                     GLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLAML
                     RSVLAVDRVTV"
BASE COUNT         3461 a         3525 c         3403 g         3402 t
ORIGIN      
        1 gaaaatctcg ccaagcggtg tctggctgtt attgatcgcc cagtctgccc cgctctcttt
       61 cgctttttcc agtcgttcat caatgcgatc ggcaacaatc acatttttaa cgttatagac
      121 gccttttaat acctgaacga tcgtcaggcc gattggaccg gcaccataaa ccagaacggt
      181 atcattttca gtcggttgac catgtccggt tacgttagcc gcaatggtaa aaggttcgat
      241 cattaccgca tattgatcgg ccactgcttc aggaattttc cacgcatttt ttgccggaac
      301 cacggcatat tcactgaaac caccgtcagc gtgcacacct aaaacagcca gtgtcgtaca
      361 aacgttcggt ttacctatag agcacggata gcaatgccca cagctgacca ccggatcgac
      421 agcaacacgt tcaccgactc tggcgctttc cacgccgtca cccaccgcat caatgacgcc
      481 aaagaattca tgtccaatga cgcgcggata tttcgcaaaa ggattatgcc cacgataaat
      541 atggctatct gaaccacaaa ttccggcaag tttcactttt actcgtactt cacccgctga
      601 cggggtgggt atttcacgtt cgacaatcgc cagttgattc ggtttttcaa ttaatatgct
      661 tttcattatc ttactcctta ccagttccac agcgtgccat cttccagacg tgcgactggt
      721 agataagcag gttcataggg atatttcgcc gccagctttt catcgaattc gatgccaaga
      781 cccggtttgt ctcccggatg catatagccg ttatcgaaag tccagttgtg cgggaagact
      841 tcgagcattt gttcggaata acccatgtat tcctggacac cgaaattggg gacccacaga
      901 tcaaagtgca gcgccgcagc catgcagact ggtgacaaat cggaaggacc gtgtgagcca
      961 gtacgtacct gatacagcga agcaaaatcg gcaatccggc gcataccggt aattccgcct
     1021 gcatgggtca gcgtggtgcg gatataatcg atgagttgct cttcaatcag ttgtttgcag
     1081 tcccagatgc tgttgaagac ttcacccact gcgatgggtg tgacggtatg ttggcgaatg
     1141 agacggaagc attcctggtt ttctgcaggc gtcgggtctt ccatccagaa catgcgataa
     1201 tcttcaatgc ttttaccaaa gcgcgccgct tcaataggcg ttaagcgatg gtgcatgtca
     1261 tgcagcaaat gttcattaaa accaaacttg ttacgtaccg cgtcaaacaa tttcggcatg
     1321 aaatcgaggt atttctccgt cgaccacagc tgctcttccg gccactgtcc tttggttgcg
     1381 ggttcataag ccagaccttt acctttcgac atgccgtagg tggttttcat accagggatt
     1441 ccgcactgca cgcggatggc tttgaatccc agctcctgat gacgggcata atcatccaga
     1501 gcttcatcaa tactgtgacc ggtggtatgg caataaacca tcaccccttc acgagacgcg
     1561 ccgccgagta actggtaaag cggcatgttg gcagctttgg ctttaatatc ccacagcgcc
     1621 atatcaaccg ctgaaatggc cgacatcgta accggaccgc gacgccagta agcaccttta
     1681 tagaaaaact gccagatatc ttcgatacgg tgcgcatcgc gaccaataag ctgcggacaa
     1741 aggtgatcct gcaaataaga ggccacggaa agctcacgtc cattgagggt ggcatcccca
     1801 aggcccgtaa taccgtcctc agtggtgatt tttaatgtga cgaaattacg ccccggacag
     1861 gtaacaaaaa cttcagcctt tacgatcttc atgttcgatt ccttgcatcg cttgtcgtga
     1921 tgcatgaaat ctacgcaact gagctactac catacaagta taaagatcga aaaaagccgg
     1981 agtgatcaca aaaaaaggcg tatatttgcg ctgtgaatgg ttgacaaaag atgaaataga
     2041 ataccttttg tcagctgaca cttcctctta tcttattgat aaaatggatt tatgttccta
     2101 cgtgcgcccc cagcccgcaa caatgatcaa catgccgcaa agcgcaatca acgcacccgt
     2161 ccagtcataa agagtcagtt tcacgccatc cacaacgcgc agccacatca acgccgtgca
     2221 gacataaacg ccaccataag ccgcgtaaac acgcccactc gccgctggat gcaacgttaa
     2281 caaccagaca aacagcgcca gtgaaatccc cgccggaagc aacagccaga tactggcgtt
     2341 tcgttttaac cacaaccagg gcagaaagca tccaataatt tcacacagcg cagtagcaaa
     2401 aaatagtaac gttgttttaa tcatctttgt ctcttattga catcatgtat agttataggg
     2461 cgacataata tcatcaatat aaacaccctc ctggtacgtt ttgcgtccgc agtggatggt
     2521 gtagaatcac ctttaatcat tcatacaggg aatgaattat gaaaatcact ctcagcaaac
     2581 gaatcggcct gctcgctatt ctgctgcctt gcgcactggc attgagcaca actgttcatg
     2641 ccgaaactaa caaactggtg attgagtctg gcgacagtgc acaaagccgc cagcacgccg
     2701 ctatggaaaa agagcaatgg aatgacacgc gcaatctgcg ccagaaagtg aataaacgca
     2761 ctgaaaaaga gtgggataaa gccgacgccg cttttgataa ccgcgataaa tgtgagcaaa
     2821 gcgccaacat caatgcctac tgggagccca atactttgcg ctgcctggac cgtcgaactg
     2881 gccgcgttat taccccctaa cctgttattg atttaaggaa tgtaaggaca cgttatgcca
     2941 agcgcccaca gtgttaagct acgcccgctg gagcgtgaag atttacgcta tgtacatcaa
     3001 ctcgacaaca acgccagtgt gatgcgttac tggtttgagg aaccctacga agcctttgtt
     3061 gaactctctg atctgtatga taagcatatt cacgatcaga gcgaacggcg ctttgtggtg
     3121 gaatgtgacg gcgaaaaagc cggtctggtg gagctggtgg aaattaacca tgttcatcgc
     3181 cgcgcagaat ttcagataat tatctccccg gagtatcagg ggaaaggtct ggcaacccgt
     3241 gccgccaaat tagcaatgga ctatggcttt accgttctca atctctataa gctgtatctg
     3301 atcgttgata aagagaatga aaaagcgatt cacatttacc gcaagcttgg cttttcggtt
     3361 gaaggtgaat tgatgcacga gttctttatt aatggtcaat atcgtaatgc cattcgcatg
     3421 tgtatattcc agcatcagta tctggcagag cacaaaacac cgggtcagac tctcctgaag
     3481 ccgaccgcac aatagcatta ataataatcg atcgtatttt tgatggtgta aacccgttcg
     3541 acggcgggtt ttactccttc atcaacaatg attagctgac agtccaccgg attagcgtga
     3601 ctgtcatatt cacagctctg ttttacatta ccaacccgtt gattattcag taaagtaacc
     3661 gctgtgtaat ctaatttttt gatcggatcc gttgatggcg tggcgctgac agataatgtt
     3721 ttgtcgttac ttttcgtggt tttacccagc ggataaccct gatcatcata gcgatattcc
     3781 atctgcattt gtttgctgct ggctttaatc acgaagccat tatcatcggt ttcccaactc
     3841 accccggcag aaggtaattc tgctagctgg cattttccct gtaaacgtac tctcttctcc
     3901 agcgtctcgg catcacggta ataattggcg tccagtacca gagcgaccac ggtattattt
     3961 tccagatcca gtaattcgag tgaatcaaaa cagccttctt ccgacaaagt cccagaaaca
     4021 cgtttcgtca cttcaccttg ctcatccatt aatgtctgag tgaaatcttt taccggacca
     4081 cgcagcggat caaaatcgaa ttcattagag aaactggcca tctcaggggt aaatgaaagt
     4141 gttacctctg tgcggtcaca tcctgtgagg aatatcgcga gtaagcatgg tagtaatttg
     4201 tatttcacaa cagtcaccag agagtagaga tgattctcaa tcatagtagc aaatacagta
     4261 ctttacacgt taaatgctat gcttaaagaa gttatcttcg cgtaaggagc ttatgatgaa
     4321 actctcaacc tgctgtgccg cgcttctgct cgccctcgcc tcacccgtgg tactcgccgc
     4381 gccgggatcc tgtgagcgca tacagagcga tatatcacag cgcattatca ataatggcgt
     4441 acccgaaagc agcttcacgt taagtattgt acccaatgac caggttgatc agcctgattc
     4501 ccaggtcgtc ggccattgcg ctaatgatac gcataaaatt ctctataccc gcaccaccag
     4561 cggtaacgtc tctgctcccg cgcagtctac ccaggatggc gcgcctgccg aaccgcagta
     4621 atacattatc gactgaacgc cggatacgac acatccggcg atttgaacat acaacataat
     4681 cccaccttat tactcatacc cttctattga tatggattaa taattcttaa cccaaaatgg
     4741 gtagactccc tctattgtta gcgcgctaaa tattcaatat ataaacattt atataacgat
     4801 aaagaacagg gagtgagtta tgtccaaaaa tgaccgaatg gtgggcatca gccgcagaac
     4861 actcgttaaa tcaaccgcga taggttctct ggcgctggct gcaggcggtt tttctttacc
     4921 gttcaccctg cgcagtgcag cggcaacggt acaacaggcc agcgaaaaag tgatctgggg
     4981 tgcctgttcc gtcaactgtg gtagccgctg tgcacttcgt ctacatgtta aagataatga
     5041 agtgacctgg gtggaaactg acaataccgg cagcgatgag tacggcaacc atcaggtacg
     5101 cgcctgtttg cgcggtcgct ccatccgccg gcgtattaat catcccgatc gcttgaatta
     5161 cccaatgaaa cgcgtgggta cacgcggtga aggcaaattc gaacggatta gctgggatga
     5221 agccctggat actatcgcca gtagcctgaa aaaaaccgtc gaacaatatg gcaatgaggc
     5281 tgtatatatt cagtactctt cggggattgt tggcggcaat atgacccgct cttcgccatc
     5341 agcctcggcg gtcaaacgcc tgatgaactg ctacggcggt tcactcaatc agtatggctc
     5401 ctacagcacc gcgcaaattt cctgtgccat gccctacacc tacggcagta atgatggcaa
     5461 cagcaccacg gatattgaaa acagcaagct ggtcgtgatg tttggtaaca acccggcaga
     5521 aacccgcatg agcggcggtg gcatcactta tcttcttgaa aaagcgcgcg agaaatcgaa
     5581 cgccaaaatg attgttatcg atccgcgata taccgatacg gctgctggtc gtgaagatga
     5641 atggctccct attcgcccgg gcaccgatgc cgcgctggtt gcgggtattg cctgggtatt
     5701 gattaacgaa aatctcgttg atcaaccttt tctcgataaa tactgcgtcg gctatgacga
     5761 aaaaacctta cccgcagatg cacccaaaaa tggtcactat aaagcctata ttcttggtga
     5821 aggtgatgat aacacagcga aaacgccgca gtgggcttcg caaattaccg gtatcccggt
     5881 agaccgtatc atcaaactgg cgcgtgaaat tggcacagca aaacccgctt atatctgcca
     5941 gggctggggg ccgcaacgcc aggcaaacgg cgaactgact gcacgcgcta ttgctatgtt
     6001 acctattttg acgggcaatg tcggcatcag cggcggaaat agtggcgcgc gtgaatcgac
     6061 ctataccatt accatagaac gcctgccggt gctggataat ccggtcaaaa cgtcaatctc
     6121 ctgcttcagc tggacagatg ctatcgatca tggtccgcaa atgacggcaa tccgcgacgg
     6181 cgtccgcggc aaagataaac tggatgtgcc cattaagttc atctggaact acgcgggaaa
     6241 taccctcgtt aatcagcatt ctgacatcaa caaaacgcat gaaattctgc aagacgaatc
     6301 gaaatgcgaa atgattgtgg tcatcgaaaa ctttatgacc tcatcggcaa aatatgccga
     6361 cattctgctg ccagacctga tgaccgttga gcaggaagat attattccta acgactacgc
     6421 cggtaacatg ggatatctca ttttcctcca gccagtcacc agcgaaaaat tcgaacgtaa
     6481 accgatttac tggatcctga gtgaagtcgc gaaacgtctt ggacctgacg tctatcaaaa
     6541 gttcactgaa ggtcgcacgc aggaacaatg gttacaacat ctgtacgcca aaatgcttgc
     6601 caaagatccg gcgttaccgt cttacgacga actgaaaaaa atgggtatct ataagcgtaa
     6661 agatcccaat ggtcattttg tcgcctacaa agcatttcgt gacgaccccg aggcaaatcc
     6721 acttaaaaca ccttccggta agattgaaat ttattccagc aagctggcgg aaattgcccg
     6781 tacctgggaa ctggaaaaag atgaagtgat aagcccactg ccagtttatg cctctacctt
     6841 tgaaggctgg gactcccctg aacgtagaac cttcccactt caactgtttg gtttccatta
     6901 caaatcccgt actcactcga cctacggcaa tattgatctc ctgaaggctg cctgccgtca
     6961 ggaggtgtgg atcaacccta tagatgcgca gaaacgtggg attgccaacg gcgatatggt
     7021 gcgggtgttt aaccatcgtg gcgaagttcg gctaccagcc aaagtgacac cacgcattct
     7081 ccctggggtt agcgctatgg gccagggagc ctggcacgag gccaatatgt ctggcgataa
     7141 aatcgaccat ggcggctgtg tgaatacgct aaccactctg cgcccttcac cactggcgaa
     7201 gggaaacccg cagcacacta atctggtcga gatcgaaaaa atataaccca cgacaaccat
     7261 aaattctggc atgacatttt gtttgaaaag caataagtga gtaatgatga aaatccatac
     7321 cacagaggcg ctcatgaagg ctgaaatcag ccgtagaagt ctgatgaaaa cctccgcact
     7381 tggcagtctt gcgctggcaa gcagtgcttt cactctgcca ttttctcaaa tggtccgggc
     7441 ggcagaggct ccggtagaag agaaagcggt ctggagttcc tgcaccgtta actgcgggag
     7501 ccgctgtctg ttacgtttgc atgtgaaaga tgacaccgtg tactgggtgg agtctgatac
     7561 gacaggtgac gacgtctacg gtaatcatca ggttcgcgcg tgtttacgcg ggcgctctat
     7621 ccgccgacgg atgaatcatc ctgataggtt gaaatatccc atgaagcgcg tcggcaagcg
     7681 cggtgaaggt aaatttgaac ggataagttg ggacgaagcc ctggatacca tcagtgataa
     7741 tcttcggcgg atcctaaaag attacggcaa tgaggctgta catgtcctgt acggaacagg
     7801 cgtagatggc ggaaacatca ccaactcaaa cgtcccgtac cgtctgatga actcttgcgg
     7861 tggttttctc agtcgctatg gcagctacag taccgcacag atcagtgccg caatgagtta
     7921 tatgttcggt gccaatgatg gcaacagccc ggatgatatc gccaatacga aactggtcgt
     7981 tatgttcgga aataacccgg cagaaacgcg gatgagcggc ggtggtgtca cttactacgt
     8041 cgagcaagcc cgcgaacgtt caaacgcacg catgatcgtc atcgatccac gttataacga
     8101 cacagctgcc gggcgtgaag atgaatggct gcccattcgc ccaggcaccg atggcgcact
     8161 ggcctgtgcg attgcctggg tactgattac tgaaaacatg gtcgatcagc catttctcga
     8221 caaatattgt gttggttacg atgaaaaaac tctgcccgcc aacgcaccac gtaacgcgca
     8281 ttataaagcc tatattctgg gcgaagggcc tgacggcata gctaaaacgc cggaatgggc
     8341 agcaaaaatc accagcatcc cggcagaaaa aattatccag ttggcacgag agatcggttc
     8401 agcaaaacct gcttatattt gtcagggttg ggggccacaa cgacattcca acggcgagca
     8461 aacgtcccgc gctattgcca tgctttccgt tctcaccggt aacgtcggca taaacggcgg
     8521 caactcaggt gtacgcgaag gtagctggga tctgggggta gaatggttcc cgatgcttga
     8581 gaaccctgtt aaaacgcaga tttccgtctt tacatggaca gatgccatcg accatggtaa
     8641 ggaaatgacc gcgacccgtg atggtgttcg tggaaaagaa aaactggatg tccccatcaa
     8701 gtttttatgg tgctacgcca gtaacacatt gatcaatcaa catggcgaca tcaatcacac
     8761 ccatgaggtg cttcaggatg acagcaagtg cgagatgatt gttggcattg accacttcat
     8821 gacggcctcg gctaagtatt gcgatatcct gttgcccgac ctgatgccaa cagagcaaga
     8881 agaccttatc tctcatgaat ctgcagggaa tatgggctat gtgatcctcg cccaacccgc
     8941 aacctctgca aaatttgaac gcaaacccat ctactggatg ctgagtgaag tcgccaaacg
     9001 cttaggacca gacgtttatc aaacctttac tgaaggtcgc agtcagcatg aatggatcaa
     9061 atatctccat gcgaaaacga aggaacgtaa ccctgagatg cccgactacg aggagatgaa
     9121 aacgaccggg atctttaaga aaaaatgccc ggaagaacac tacgtcgctt tccgcgcatt
     9181 ccgtgaagat ccccaggcaa acccgttgaa aacaccttcg gggaaaatcg aaatttattc
     9241 tgaacgactg gcgaagattg cggatacctg ggaattgaaa aaagatgaaa ttattcatcc
     9301 ccttccagct tatacgccag gttttgatgg ctgggacgat cccctgcgga aaacctatcc
     9361 actgcagtta acgggcttcc actacaaggc gcgtacccac tccagctacg gcaatattga
     9421 tgtgttacag caggcctgcc cacaagaggt gtggatcaac cccattgatg ctcaggctcg
     9481 cggtatccgt catggcgata ccgtgcgggt atttaacaat aatggagaaa tgctgattgc
     9541 cgcaaaagtg actccgcgta ttctgcctgg cgttaccgcc atcgggcaag gtgcgtggct
     9601 taaagcggat atgtttggtg accgggtcga tcacggcggc agtatcaata ttctgacctc
     9661 tcaccgccct tcaccgctgg caaagggaaa cccgtcgcac agcaatcttg tccagatcga
     9721 aaaggtttaa agagtagccc atgaccacac aatatggatt ttttatcgat tccagccgct
     9781 gtactggctg taaaacctgc gaactggcgt gcaaagattt taaagatctt ggcccggaag
     9841 tcagtttccg ccgtatttat gaatatgctg gcggcgactg gcaggaagat aatggcgtct
     9901 ggcaccagaa cgtgttcgct tattatctct ccatctcctg taaccattgc gatgacccag
     9961 catgtacaaa agtctgcccc agcggcgcaa tgcataagcg tgaagatggc tttgtggtgg
    10021 ttgatgaaga tgtttgtatc ggctgccgct actgccacat ggcttgtccg tacggcgcac
    10081 cacagtacaa tgctgaaaaa gggcacatga cgaagtgcga tggttgttat tcgcgcgtcg
    10141 ccgaggggaa acaacccata tgcgtcgaat cctgcccgct gcgtgcactt gagttcggtc
    10201 caattgaaga gctgcgccag aagcacggca ctctggctgc cgtcgcgcca ctgccgcgcg
    10261 cgcatttcac aaaacccaat atcgttatca aacctaacgc caacagccgc ccgaccggcg
    10321 ataccactgg ttatctggct aatccggagg aagtgtaaaa tgggaaatgg atggcatgaa
    10381 tggccactgg ttatctttac cgtattgggt caatgcgtag taggcgcgct gattgttagc
    10441 ggaattggct ggttcgccgc caaaaatgac gcagaccgtc agcgtattgt ccgtggcatg
    10501 tttttcctct ggctgttgat gggcatcggg tttattgcct ctgtcatgca cctgggatcg
    10561 ccactgcgag cgtttaactc gctgaatcga atcggagctt ctggtctgag taacgagatt
    10621 gctgccgggt ccatattttt tgccgttggc ggactatggt ggctggtggc ggtcatcggt
    10681 aaaatgccgc aagcgttggg gaaactctgg ttacttgtca gcatggcgct cggcgtcatt
    10741 ttcgtctgga tgatgacctg cgtgtatcaa atcgacaccg tgccaacctg gcataacggt
    10801 tacactacgc tggctttctt cctgacggta ttgttgagcg gtccaatact tgctgccgca
    10861 attctgcggg cagctcgcgt tacatttaat accacaccat ttgccatcat tagcgttctg
    10921 gcgctgatcg cttgtgccgg ggtgattgtg ctgcaaggtt tgtctctggc gtgtattcac
    10981 tcatccgtac aacaagccag cgcactggta ccagattacg cctccttaca ggtctggcgt
    11041 gtggtattgt tatgcgcagg tctaggatgc tgggtttgtc cgctcattcg tcgccgtgaa
    11101 cctcacgttg ccggacttat tctggggtta atcctcattc ttggcggtga gatgatcggg
    11161 cgtgtgctct tttatggcct gcacatgacg gtaggaatgg ctattgcagg ttaacaaatt
    11221 ggtgcggggc aacccgcaca tttcaggatg ttaggaatga cccatttttc acagcaagat
    11281 aatttttctg tcgcggcgcg cgtgttgggc gcgctgtttt attacgctcc agagagtgcg
    11341 gaagccgcac ctctggttgc ggtactcacc agtgacggct gggaaactca gtggcctcta
    11401 ccagaagcgt cattagcgcc gctggtgacg gcttttcaga cacagtgtga agagactcac
    11461 gcccaggcct ggcagcgttt gtttgtcggc ccgtgggcac tgccgtctcc gccgtggggg
    11521 tcggtctggc tggatcgcga atctgtgctg tttggcgatt caacattggc acttcgtcag
    11581 tggatgcgcg agaaaggcat tcagtttgaa atgaagcaaa acgaaccgga agatcatttt
    11641 ggttcactgc tgttgatggc tgcgtggttg gcggagaatg gtcgccagac agaatgcgaa
    11701 gaattgctgg catggcacct ttttccgtgg tcaacacgtt ttcttgatgt ttttatcgaa
    11761 aaagcagaac accctttcta ccgtgcactg ggtgaactgg ctcgcttaac gctggcgcaa
    11821 tggcaatcac aactgttgat tcctgtcgcg gttaaaccgc tgtttcgata ggatcaccgt
    11881 aatattgccg gatggtgacg attacgcgca atccggcaat aataggttac agtgtcacgt
    11941 ttttttatct cttaaagcac gcactgcttt tgcggctggc ctcttttgtc gcaaaatagt
    12001 cgcccgtgtt tcattgccca tttctgctca tgcatcatct acacatctat ccggatctgc
    12061 gcactatgtt ccgccgtctg cttatcgcaa cagtcgtcgg tattctcgcg gcctttgccg
    12121 ttgccgggtt tcatcatgcg atgctgctac tggagtggtt gttcctcaat aatgactccg
    12181 gcagtctggt caatgcagcg acaaaccttt ccccctggcg acggctgcta actccggcgc
    12241 tcggcggact ggcggcgggt ttgttgctga tgggctggca gaaatttacc caacaacgcc
    12301 ctcatgcgcc gaccgattac atggaagcgt tgcaaaccga tggacagttc gattacgcag
    12361 caagcctggt taaatcgctt gcctcgctgc tggtagtaac cagcggcagt gcaattggtc
    12421 gcgaaggtgc gatgattctt ttagctgccc ttgccgcctc ctgttttgcc caacgtttta
    12481 cgccacgcca ggagtggaaa ttatggatcg cctgtggggc cgcggcggga atggctgcgg
    12541 cctatcgtgc cccgcttgct ggcagtttat ttatagccga agtgctgttt ggcactatga
    12601 tgttggcctc tctcggcccg gtgattattt ccgccgtcgt ggcattgctg gttagcaatc
    12661 tgattaatca tagcgacgcg ttactctaca acgtacaact ctcagtgacg gttcaggctc
    12721 gtgactatga gctgattatc agtacaggtg tgctggcagg tctgtgcgga ccactgttgt
    12781 taacgttaat gaacgcctgt catcgtggat ttgtgggtct caaacttgcg ccgccctggc
    12841 aactggcact aggcgggttg atcgtgggtc tgctttccct gttcacacct gcagtgtggg
    12901 gcaacggcta tagcaccgta caatcctttt taaccgcccc gccactgtta atgatcattg
    12961 ccgggatctt cctttgtaaa ctgtgtgccg tgctggcgag tagcggttcc ggcgcacccg
    13021 gtggtgtctt tacaccgacg ctatttatcg gtcttgccat tggcatgttg tatggtcgta
    13081 gcctgggatt atggttccct gatggcgaag aaattacgct tttactcgga ttgaccggga
    13141 tggcgacact gttggcggca accacgcacg cgccgattat gtcgacgttg atgatatgtg
    13201 aaatgaccgg ggagtatcag ctactccccg gtttattgat tgcctgcgta attgcgtcgg
    13261 taatctcgcg gacgttacac cgtgactcta tctaccgcca gcacactgcg cagcatagct
    13321 aagcggatgt attgccccag ttcacgctgt tcggcgcgcg gcagataagg cagttcacca
    13381 atgagcggtg ccggaagttt tttaccgagc acatcaatga tttccgcata atgcgccagt
    13441 cctgggttga ttcggttagc cacccagcca atgagcggca gcccgtcgtt ggcaatcgcc
    13501 tgagctgtta gcagtgcatg gttaatgcaa ccttcctgaa taccgacaac catcaacacc
    13561 ggcagttgtt cctgcaccac ccattcagag agtggacgca aatcattcat cagactgcgc
    13621 cagccgccag tcccttctac cacgacatga tcgacttttt cggtcaggtt tgccaggccg
    13681 tttgaaatga gggtgtaatt gattgggcaa ctgtgcgcca cgctactttc ttcttcgctt
    13741 aacgcgatag gattaactgc ttcataaggc agttcgatgg ttgaaacact c
//
LOCUS       DAMZET010000112        13697 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.112, whole genome shotgun sequence.
ACCESSION   DAMZET010000112 DAMZET010000000
VERSION     DAMZET010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.112"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            534..827
                     /gene="iss"
                     /locus_tag="POB34_003302"
     CDS             534..827
                     /gene="iss"
                     /locus_tag="POB34_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HDI5920193.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGLLGFITFGIYTPLEARVYCSQ"
     gene            complement(885..1060)
                     /locus_tag="POB34_003303"
                     /pseudo
     CDS             complement(885..1060)
                     /locus_tag="POB34_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309650.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            1638..2740
                     /locus_tag="POB34_003304"
                     /pseudo
     CDS             1638..2740
                     /locus_tag="POB34_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356822.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3976..5091
                     /gene="iroB"
                     /locus_tag="POB34_003305"
     CDS             3976..5091
                     /gene="iroB"
                     /locus_tag="POB34_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="salmochelin biosynthesis C-glycosyltransferase
                     IroB"
                     /protein_id="HDI5920194.1"
                     /translation="MRILFVGPPLYGLLYPVLSLAQAFRVNGHEVLIASGGQFAQKAA
                     EAGLVVFDAAPGLDSEAGYRHHEAQRKKSNIGTQMGNFSFFSEEMADHLVEFAGHWRP
                     DLIIYPPLGVIGPLIAAKYDIPVVMQTVGFGHTPWHIKGVTRSLTDAYRRHNVGATPR
                     DMAWIDVTPPSMSILENDGEPIIPMQYVPYNGGAVWEPWWERRPERKRLLVSLGTVKP
                     MVDGLDLIAWVMDSASEVDAEIILHISANARSDLRSLPSNVRLVDWIPMGVFLNGADG
                     FIHHGGAGNTLTALHAGIPQIVFGQGADRPVNARVVAERGCGIIPGDVGLSSNMINAF
                     LNNRSLRKASEEVAAEMAAQPCPGEVAKSLITMVQKG"
     gene            5231..8890
                     /gene="iroC"
                     /locus_tag="POB34_003306"
     CDS             5231..8890
                     /gene="iroC"
                     /locus_tag="POB34_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="salmochelin/enterobactin export ABC transporter
                     IroC"
                     /protein_id="HDI5920195.1"
                     /translation="MPANHTPTPAQSWIVRLARVCWERKKLSVIVVVASVSTILLAAL
                     TPLLTRQAVNDALAGNPARLPWLACGLLLIAFFDFIGNYVRRGYAGMLSLWVQHTLRG
                     RVFDSIQKLDGAGQDALRTGQVISRTNSDLQQVHTLLQMCPVPLAVFTYYIAGIAVML
                     WMSPAMTLIVVCVLVCLAITALRARRRVFAQTGLASDQLANLTEHIREVLAQISVVKS
                     CVAEMRETHWLDRQSRQIVRVRIGAVISQAMPGATMLALPVLGQIVLLCYGGWSVMHG
                     RIDLGTFVAFASFLAMLTGPTRVLASFLVIAQRTQASVERVFALIDTRSQMEDGTESI
                     NSQVVGLELENMSFDYHHGDRHILSNISFSLRAGETVAVVGASGSGKSTLLMLLARFY
                     DPCSGKIWLNTSEGRQNLRDIRLEALRRRVGIVFEDAFLFAGTVAENIAYGHPQATAD
                     DIRRAAAAAGASDFINALPKGFDSLLTERGTNLSGGQRQRIALARALITAPDVLILDD
                     TTSAVDAVTEAEINTALGRYADEGHMLLVIARRRSTLQLASRVVVLDKGRMVDTGTPA
                     ELEARCPAFRALMTGDSDFLATSHNSHNELWPAEPATQDDVTDTGDKGFVARMTRVPE
                     NAVQQALAGKGRKVTSLLKPVAWMFVIAALLIALDSAAGVGVLILLQHGIDSGVATGD
                     MSIIGLCALLALCLVIVGWCSYSLQTVFAARAAESVQHSVRLRSFGHMLRLGLPWHEK
                     HADSRLTRMTVDVDSLARFLQNGLAGAATSLVTMFAIAATMFWLDPFLALTALSAVPV
                     AALATMIYRRLSTPAYAQARLEIGKVNSTLQEKVSGLRVVQSHGQQELEGARLRALSE
                     RFRATRVRAQKYLAVYFPFLTFCTEASYAAVLLVGASQVAAGEMTAGVLAAFFLLLGQ
                     FYGPVQQLSGIVDAWQQATASGKHIDELLATEGTEHLGSSSVLPVTGALHLDEVTFSY
                     PDSHEPALNKLTLTIPEGMVVAVVGRSGAGKSTLIKLIAGLYFPTHGNIRIGVQMLDD
                     ASLTEYRRQIGLVDQDVALFSSDIAENIRYSRPSATNEDVEIASQRAGLYEMVCNLPQ
                     GFRTPVNNGGADLPAGQRQLIALARAQLANAHILLLDEATSCLDRTSEERLMSSLTDV
                     VHAGKHSALIVAHRLTTAQRCDLIAVIDKGLLAEYGTHEQLLSAGGLYTRLWHDSVSS
                     TALHRQHNMKEETPG"
     gene            8994..10223
                     /gene="iroD"
                     /locus_tag="POB34_003307"
     CDS             8994..10223
                     /gene="iroD"
                     /locus_tag="POB34_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore esterase IroD"
                     /protein_id="HDI5920196.1"
                     /translation="MLNMQQHPSAIASLRNQLAAGHIANLTDFWREAESLNVPLVTPV
                     EGAEDEREVTFLWRARHPLQGVYLRLNRVTDKEHVEKGMMSALPETDIWTLTLRLPAS
                     YCGSYSLLEIPPGTTAETIALSGGRFATLAGKADPLNKMPEINVRGNAKESVLTLDKA
                     PALSEWNGGFHTGQLLTSMRIIAGKSRQVRLYIPDVDISQPLGLVVLPDGETWFDHLG
                     VCAAIDAAINNGRIVPVAVLGIDNINEHERTEILGGRSKLIKDIAGHLLPMIRAEQPQ
                     RQWADRSRTVLAGQSLGGISALMGARYAPETFGLVLSHSPSMWWTPERTSRPGLFSET
                     DTSWVSEHLLSAPPQGVRISLCVGSLEGSTVPHVQQLHQRLITAGVESHCAIYTGGHD
                     YAWWRGALIDGIGLLQG"
     gene            10308..11264
                     /gene="iroE"
                     /locus_tag="POB34_003308"
     CDS             10308..11264
                     /gene="iroE"
                     /locus_tag="POB34_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore esterase IroE"
                     /protein_id="HDI5920197.1"
                     /translation="MYAREYRSTRPHKAIFFHLSCLTLICSAQVYAKPDMRPLGPNIA
                     DKGSVFYHFSVTSFDSVDGTRHYRVWTAVPNTTAPASGYPILYMLDGNAVMDRLDDEL
                     LKQLSEKTPPVIVAVGYQTNLPFDLNSRAYDYTPAAESRKTDLHSGHFSRKSGGSNNF
                     RQLLETRIAPKVEQGLNIDRQRRGLWGHSYGGLFVLDSWLSSSYFRSYYSASPSLGRG
                     YDALLSRVTAVEPLQFCAKHLAIMEGSATQGDNRETHAVGVLSKIHTTLTILKDKGVN
                     AVFWDFPNLGHGPMFNASFRQALLDISGENANYTAGCHELSH"
     gene            complement(11309..13486)
                     /gene="iroN"
                     /locus_tag="POB34_003309"
     CDS             complement(11309..13486)
                     /gene="iroN"
                     /locus_tag="POB34_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001222186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore salmochelin receptor IroN"
                     /protein_id="HDI5920198.1"
                     /translation="MRINKILWSLTVLLVGLNSQVSVAKSSDDDNDETLVVEATAEQV
                     LKQQPGVSVITSEDIKKTPPVNDLSDIIRKMPGVNLTGNSASGTRGNNRQIDIRGMGP
                     ENTLILIDGVPVTSRNSVRYSWRGERDTRGDTNWVPPEQVERIEVIRGPAAARYGSGA
                     AGGVVNIITKRPTNDWHGSLSLYTNQPESSDEGATRRANFSLSGPLAGNALTTRLYGN
                     LNKTDADSWDINSPVGTKNAAGHEGVRNKDINGVVSWKLNPQQILDFEAGYSRQGNIY
                     AGDTQNSSSSAVTESLAKSGKETNRLYRQNYGITHNGIWDWGQSRFGVYYEKTNNTRM
                     NEGLSGGGEGRILAGEKFTTNRLSSWRTSGELNIPLNVMVDQTLTVGAEWNRDKLDDP
                     SSTSLTVNDSDISGISGSAADRSSKNHSQISALYIEDNIEPVPGTNIIPGLRFDYLSD
                     SGGNFSPSLNLSQELGDYFKVKAGVARTFKAPNLYQSSEGYLLYSKGNGCPKDITSGG
                     CYLIGNKDLDPEISVNKEIGLEFTWEDYHASVTYFRNDYQNKIVAGDNVIGQTASGAY
                     ILKWQNGGKALVDGIEASMSFPLVKDRLNWNTNATWMITSEQKDTGNPLSVIPKYTIN
                     NSLNWTITQAFSASVNWTLYGRQKPRTHAETRSEDTGGLSGKELGAYSLVGTNFNYDI
                     NKNLRLNVGVSNILNKQIFRSSEGANTYNEPGRAYYAGVTASF"
BASE COUNT         3280 a         3530 c         3526 g         3361 t
ORIGIN      
        1 gtgcacgaat cctagaggca gaatgtttct ccacatgctt gtggagaaac cgccgcattt
       61 tgcccgttct gtacgataca ccgtagccag agataagata ttctgttgct gtatgctaca
      121 tacattacac tatacgaaat caggtctgtt caatcagtaa ggaaataaac ggacaaacgt
      181 tatgtaaccc agacagagat atattgtctg ggaaaatagt ggcttttgtg gcgaaaacta
      241 gtaaaacagc aacccgaacc acttgatgtg catcgttttt gattattccc gtatactctt
      301 gcagaaggag ttctccgtcg ggctactgtc atggttaatg cggggaatat ggcgacaata
      361 caacatacct aaaagagtaa tggacagatg aagcggttta ttcatttccc atgattctga
      421 gtacctacca agtctgagta accactttta tacttttaat tttcgttcat ttagctatcg
      481 tttaattatt atcacatagg attctgccgt ttttaacaat gcaggataat aagatgaaaa
      541 aaatgttatt ttctgccgct ctggcaatgc ttattacagg atgtgctcaa caaacgttta
      601 ctgttggaaa caaaccgaca gcagtaacac caaaggaaac catcactcat catttcttcg
      661 tttcgggaat tggacaagag aaaactgttg atgcagccaa aatttgtggc ggtgcagaaa
      721 atgttgttaa aacagaaact cagcaaacat tcgtaaatgg attgctcggt tttatcactt
      781 ttggcatcta tactccgctg gaagcccggg tatattgctc acaatagttg cccatcgata
      841 tggggagctc atctgcactg ttcattaata tacttctggg ctccctacag ttgtttttgc
      901 atagtgataa gcctctctct gagggaggaa ataatcctgt tcagcgatgt ctgccagtcg
      961 ggggggctgc attatccacg cccgaggcgg tggtggcttc acgcggggat gggcagattg
     1021 atctgatatg caaccgacga cgaccagcgg caacatcatc acgcagagct tcattttcag
     1081 atttgggcca ccttttgatt tctcgtttaa cttttgcagt caccagactg tgatttattt
     1141 taacaaatca atagctttca taaaattctc gaagctgaaa gcttaactga accaaccagc
     1201 ctggctggtg ttttctaaag acaaaaaggc catcagcaaa acactgatag tctgaatcac
     1261 ctttatcaat catgtatgaa gaaaatatgc gcaattagtt gacagtgatt atcattttca
     1321 ttaaaaagag cgcgtagcat tcttatttca tgaggaaatt tacccgccaa caacctgagt
     1381 agcgaaagct gttcatcccc aatatttttg ctggcgggtt cttttttcta ctgtcctcct
     1441 ccccagagga agcattccca ttaatcatta ctggcgagga ggcaagagaa aaagtcggtt
     1501 ctttgtccct aaataacagc atcatttcag tccatgcaaa atctgattta ccagctcaca
     1561 taataacctg atgatacaca gtgtaagttc acagagatat tgcaattgcc tccggataag
     1621 taaggggaga ttgcactatg caaatgcagc acctgaggtt ggctatccta agcagccgat
     1681 tatgcgttga ggctttcagt gatggctgat atagcaacaa tgagaatgaa ggctctgcac
     1741 ttctgggata aacacggtat ttctgcagct tctgaagcct ttggcgtatc ctgccgcacg
     1801 ctttactggt ggcgtcagtt actgatcaag ggaggacctg aggggctaat cccgcacagt
     1861 aaggcacctc tggtgcgacg aaaaaagcac tggcatcccg atgtgctgaa agagattcga
     1921 cgcctcagga cagagctgcc gaacctcggt aaagagcaga tttttgttcg cctgaagccc
     1981 tggtgcgcat aacgccatca gacctgcccg agtgtttcca ccatcggcag aatgatcgcc
     2041 accgcacacg ataaaatgcg gatgatacca gtacgtctga gctcgcgggg taaggctctg
     2101 cttgtcaaaa agcgaaccac cccccccccc ccgcaggcca aagcactacc gtccggtaaa
     2161 gacaggagag ctcattggga tggacgccat tgagctcaga atgggtgaaa tgcgtcgcta
     2221 tatcattacc atgatcgacg aacacagcga ttatgtactg gcgctggctg tgccgtcgct
     2281 caacagtgat atcgtcaatc actttttcag ccgtgcagcc cggctgttcc ctgtcggtat
     2341 cagccagatc atcacagata acgggaaaga gttcctggga agctttgaca aaacgctgca
     2401 ggaagccgcc atcaaacacc tctggaccta tccctacacg ccaaaaatga acgctatctg
     2461 tgaacgtttt aaccgggcat taagagagca attcattgaa tttaatgaaa ttttactctt
     2521 tgaggatctg gcgttgttta acctgaaact ggcggaatat ctggcgctgt ataacagcaa
     2581 aagactccac aaggcgctcg cactaacgac gcccgtggaa tatattttaa aagagaacaa
     2641 aaattgcaat atgtggtgga cccatacaca gcaaacgcac aggccgcccc ggttgggtag
     2701 atatgtggga tggctggttc agacttcagg atatggttga ggctggtgat gaagtctctt
     2761 tatcaggaga tctgatcaag agacagccgc gcagggggat tcactatgct ggattctttt
     2821 aacaaattct ccgctaacca aaaaatagac tttttgcatt cgtaaatgcg ttaagagata
     2881 atttttaaag ttccatcaat ctcccggcca gttcgtctcg cggcattact cactatccgc
     2941 acaacttcag taagatcatg actttttcaa tcgttatctg ttgaccaagc tttgaaacca
     3001 ctagcgtccc gtcaacctga cctggaatcg tcgagaaccg cccatagttg tgaattggct
     3061 agagtgtgat ggaaagcaac gctaaacgac ctagtcacac attctctttt ccctaacaat
     3121 gaccatttag tgaaataacg tatccaaaat gatgacgtga aagcctgata ctggcagcga
     3181 aaagccgaaa aaactcacct ggtcaggagc aaaaatcgga ttattcgcca acagcgtaat
     3241 atcagcagta gttctcgaca cgcttgcgct atagacagag gatgaaacgc tgctggtcgg
     3301 agttctctat acatttttgg cgacggcaga accgctcacc aatgccttaa cgtctatcag
     3361 ccttccggtg agttggaaca gcatgctcag cgtttgcaag atattctcca cggtctgtag
     3421 cagcgtaggt ttcaatttaa atatccccag atagccctct gggagcttcc ctttttatta
     3481 agttttaatt accgttgcaa tagttaaatg atattggtaa ttattatcat tagcatttgt
     3541 gtctgtgttg tttctggtaa ggctgaaagg gaacaaacat cgcctgtaca acggcgtttt
     3601 tacttacaga ccagcgaagg cgagactatc aggatgatgc atcgtgtgtc taagaaatca
     3661 cggttctttc cagattcgtt gttcggtcta acactgcaca aatgcgtcag aacatcccgg
     3721 cgtagctgtc cgacgccatt tgggctgttc aacaggacaa tggcgcgtaa gcgttaatgg
     3781 aagtggggat acggagttcc ctgttcacct gtgtatgtca cggaaaagtc tctgactgag
     3841 ctgtccataa cgctggtgaa gcacaaatgg cgcagcatct ggtaaagcag gtttaacgaa
     3901 aagtcgtgtc ctgctgctgg catatgagtg cgtcgactgc ctgatttaga tcgtcaagcg
     3961 gagagggatt ttctcatgcg tattttgttt gtaggcccgc ctctctatgg gctgttgtat
     4021 cctgtgctgt ctctggcgca ggcatttcgc gttaacggtc atgaagtgct gatcgccagt
     4081 ggaggacaat tcgcacaaaa agcggcagag gccgggttgg tggtgtttga cgccgcaccc
     4141 ggtctggatt ccgaagctgg ttaccgccac catgaggcac agcggaaaaa aagtaatatc
     4201 ggaacccaaa tgggcaactt ctcattcttc agcgaagaaa tggccgacca tctggtggag
     4261 tttgccggac actggcggcc tgacctcatc atctaccctc cgctcggagt catcgggccg
     4321 ctgattgccg ccaaatatga tataccggtc gtgatgcaaa ccgtaggctt cggccatacc
     4381 ccctggcata tcaagggtgt aacgcgttcg ctcacggacg cctatcgtcg ccacaatgtg
     4441 ggggccacac cacgtgatat ggcctggatc gacgtaacac cgccgagtat gagcattctg
     4501 gaaaacgacg gtgaacccat tattccgatg cagtacgtcc cgtataacgg tggcgcggtg
     4561 tgggaaccat ggtgggagag aaggcccgag cgtaaacgtc tgctggtgag tctcggcact
     4621 gtcaaaccga tggttgacgg ccttgatcta atcgcctggg ttatggattc tgccagtgag
     4681 gtcgatgccg agatcatcct gcatatttca gccaatgcgc ggtccgattt gcgctcgctg
     4741 ccgtcaaatg ttcgcctggt tgactggata cccatgggtg tcttcctcaa cggcgcagat
     4801 ggtttcattc atcatggcgg agcaggcaat accctgacag cgctacacgc tggtattcca
     4861 caaatcgtct tcggccaggg agccgatcgc ccggtaaatg cccgcgttgt tgccgaacgc
     4921 ggctgcggga ttatccctgg cgacgtcggc ctgtcgagta acatgatcaa tgctttcctc
     4981 aacaatcgct cacttcgcaa agcctctgaa gaggtagcag cggaaatggc ggcgcaaccc
     5041 tgcccaggtg aagtggcgaa aagcctgatc acaatggtac agaaagggta accactacac
     5101 cttcatgata atcatatgcc agcacccatc caggagtcat gcatccataa caatgcggga
     5161 ccggagcgtg ctggcttttt tgcggaacga tccggccagt ggatcgtatt ttacagacgg
     5221 aggaggctta atgcctgcga atcacactcc cacaccggct cagtcatgga tagttcgcct
     5281 agcgcgcgtg tgctgggaac gtaagaaact tagtgtcatc gtggtggtag cgtcagtatc
     5341 gactattttg ctggctgcgc tgacgccact gctgacaaga caggctgtga atgacgcact
     5401 ggcgggcaat ccggcccgcc tgccgtggct ggcctgcggg ttactgttga tcgctttttt
     5461 tgatttcatc ggtaactatg tgcgccgtgg ttatgccggg atgctctcac tctgggtgca
     5521 gcataccctc agaggacggg tattcgacag tattcagaaa cttgacggcg caggccagga
     5581 tgcgctgcgc accgggcagg tgatttcacg gaccaacagc gatctgcagc aggtgcatac
     5641 cctgctgcag atgtgcccgg tgccgctggc agtgttcact tattacattg ccggcattgc
     5701 cgtgatgctg tggatgtccc ctgccatgac gcttatcgtc gtgtgcgtac tggtatgcct
     5761 ggcgatcacc gcgcttcgtg cgcgtcgtag ggtcttcgcg caaaccgggc tggcctcgga
     5821 ccaactggcg aatctcaccg aacatatacg cgaggtgctg gcacagatct cagtggtaaa
     5881 atcctgtgtg gcagagatgc gtgaaacgca ctggctcgat aggcagtcgc ggcagattgt
     5941 gcgtgtacgc atcggtgcgg ttatctcgca ggcgatgcct ggggccacca tgctggcgct
     6001 accggtgctc gggcaaatcg tcctgctgtg ctacggcggg tggtcggtca tgcacgggcg
     6061 gatcgatctc ggtaccttcg ttgcattcgc cagcttcctc gcgatgctga ccgggccaac
     6121 ccgcgtactg gcatcgtttc tggttatcgc acagcgcact caggcgtccg tggagcgggt
     6181 gtttgcactg atcgacaccc gttcacagat ggaggacggg acggagtcga ttaacagtca
     6241 ggttgtcgga ctggaactgg agaatatgag ctttgactac caccatggcg acagacatat
     6301 cctcagcaat atctcctttt ccctgcgcgc cggtgaaacc gtggcggtgg tgggcgcatc
     6361 gggttcagga aaatcgaccc tgttgatgct gctggcgcgt ttttatgatc cctgctccgg
     6421 aaagatatgg ctcaacacca gcgaaggccg acaaaatctt cgcgatatca gactggaggc
     6481 gcttcgtcgc cgggtaggca tcgtatttga agatgctttt ctgtttgccg gtacggtggc
     6541 ggaaaatatc gcctatggcc accctcaggc aacggcggac gacattcgcc gtgcggcagc
     6601 tgctgcagga gccagcgatt ttatcaacgc cctgccgaaa ggcttcgata gcctgttaac
     6661 cgaacggggt acgaatcttt ccggcgggca gaggcagcga atagcgctgg cgcgggcgct
     6721 cattactgca ccggacgtgt taatcctgga tgataccacc tcagcggttg atgctgttac
     6781 ggaagcggag attaataccg cgctgggtcg ctatgctgac gaagggcata tgctgctggt
     6841 gattgcccga cggcgttcaa cacttcagct agccagccgg gttgtggtgc tggataaggg
     6901 ccgtatggtg gataccggaa ccccggcaga acttgaagcg cgctgtccgg cgttccgcgc
     6961 actgatgacc ggcgacagcg attttctggc cacgtcccac aatagccaca acgaattgtg
     7021 gccggctgaa ccagcgacac aagacgatgt aacggatacg ggggataaag gttttgtcgc
     7081 ccgtatgacc cgcgtaccgg aaaatgcagt acagcaggcg ctggccggta aaggtcgcaa
     7141 agtcacgtca ctactgaagc ctgtggcgtg gatgttcgtc atcgccgctc tgctgatcgc
     7201 actcgattct gcggcaggcg taggggtact gatactgttg cagcacggca ttgactccgg
     7261 tgtcgccaca ggcgatatgt cgatcatcgg cctctgtgcc ctgctcgccc tgtgcctagt
     7321 cattgtgggc tggtgcagtt attctctgca gacggtcttc gccgccagag cggcggaatc
     7381 agttcagcat tcggtgcgct tgcgcagctt cggccatatg ctgcgtcttg gactcccctg
     7441 gcatgaaaag catgccgatt cgcgtcttac ccgcatgacc gttgatgtgg actctctcgc
     7501 ccgctttctg caaaacggcc ttgccggtgc ggccaccagt ctggtgacga tgttcgcaat
     7561 cgccgccacc atgttctggc tcgacccgtt cctggcgctg acggcattaa gcgcagtgcc
     7621 agtggccgca ctggcaacca tgatttatcg ccgcctcagt acccctgctt atgcacaggc
     7681 acggctggaa ataggcaaag tcaacagcac cctgcaggaa aaagtctctg gcctgcgtgt
     7741 cgtgcaatcg catggtcagc aggaactgga gggcgcccgg ctgcgcgcgt tatcggagcg
     7801 tttccgcgca acccgtgtgc gagcacaaaa ataccttgca gtctattttc cgttcctgac
     7861 attctgcacc gaggcctcct atgccgctgt tctgttagtg ggagcttcgc aggtcgccgc
     7921 tggagaaatg actgccgggg tactggcggc tttcttcctg ttgctggggc aattctatgg
     7981 gccagtgcag cagttatcag ggattgtcga cgcctggcag caggcgacag ccagcggcaa
     8041 acatattgat gaactactgg cgacagaagg cactgagcac ctcgggtcct cttcggtcct
     8101 ccctgtcacc ggtgcactgc atcttgatga ggtcacgttc agttatcccg acagtcacga
     8161 gccagctctg aacaaactta ccctgacgat cccagaggga atggttgtcg cggtcgtcgg
     8221 tcgcagcggt gcgggtaagt cgacgctgat taagctgatt gccgggttgt atttccccac
     8281 gcacggcaac atcagaatcg gtgtgcaaat gctcgatgat gcctcgctca ctgagtatcg
     8341 tcgccagatt gggcttgtcg atcaggatgt agcactgttt agtagtgata ttgcagaaaa
     8401 cattcgttat tcacggccat ccgccaccaa tgaagacgtt gaaattgcct cacagcgggc
     8461 agggctgtat gagatggtgt gcaatctgcc gcagggattc cggacaccgg tgaataacgg
     8521 cggagccgat ctgcccgcag gtcagcgcca gttgattgcg ctggcccgcg cgcaactggc
     8581 gaatgcccac atcctgctgc tcgacgaagc cacgtcatgt ctggatcgca catccgaaga
     8641 acgactgatg tcatcgttaa cagatgtcgt gcatgccggg aagcactcgg cgctgattgt
     8701 tgcacatcgt ctgaccaccg cgcaacgctg cgatctgatt gccgttattg ataaggggtt
     8761 acttgcggaa tacggaaccc acgaacagct gttatctgcg ggcggcctct atacccgctt
     8821 atggcatgac agcgtcagca gtactgcgct ccatcgccag cacaacatga aggaggaaac
     8881 cccgggatag ttactggaca cgtaatgtat taaaaacaca gtcagaagcg gcggtaccgt
     8941 gaatagccgc tttaattatt tatactgaca tccttaattt ttaaagagta tgaatgctga
     9001 acatgcaaca acatccctct gctatcgcca gcctgcgcaa ccaactggca gcgggccaca
     9061 ttgctaacct tactgacttc tggcgcgaag ctgagtcgct gaatgttcct cttgtgacgc
     9121 cagtcgaagg agcggaagat gagcgagaag tgacctttct gtggcgcgcc cgacatcctc
     9181 tgcagggcgt ttatctgcgt ctgaaccggg tgacggataa agagcacgta gaaaaaggaa
     9241 tgatgagcgc ccttcccgaa acggatatct ggacactgac actgcgttta cccgcaagtt
     9301 actgcggctc ctattcgctg ctggaaatcc cccccggcac tacggctgag acgattgcac
     9361 tgtccggagg ccgttttgcc acccttgccg gaaaggccga tccgctaaac aaaatgccgg
     9421 agatcaacgt tcggggaaac gcaaaggaat cagtgctgac acttgataaa gctcccgccc
     9481 tgtcggaatg gaacggcggc ttccacaccg gacaactgct tacctccatg cgcattatcg
     9541 ccgggaaatc tcgccaggtt cggctctata ttccggatgt tgatatttct cagcccctcg
     9601 ggctggtcgt gctgcccgat ggtgaaacct ggtttgatca ccttggcgta tgcgcggcaa
     9661 ttgacgccgc cataaataat gggcgcatcg tgcccgtggc tgtactgggc attgacaaca
     9721 ttaatgaaca tgaacgcact gagatactcg gcgggcgcag caaactgata aaggatatcg
     9781 ccggacatct gctgccgatg attcgcgctg aacaaccgca gcgtcagtgg gcagaccgtt
     9841 cgcgcacagt gctggccggg cagagcctcg gcgggatcag tgcgctaatg ggggctcgtt
     9901 acgcaccgga aacgttcggt ctggtgctca gccactctcc ttcaatgtgg tggacgccag
     9961 aaagaaccag tcgaccaggc ttgttcagcg aaaccgatac ctcatgggtg agtgagcatc
    10021 tgctttctgc cccaccgcag ggcgtacgta tcagcctgtg cgtgggatcg ctggaaggtt
    10081 cgacagtgcc tcacgttcag cagcttcacc agcggctgat taccgctggc gtcgaaagcc
    10141 attgcgcaat ctacaccggt ggtcacgatt acgcatggtg gcgcggtgca ctgattgacg
    10201 ggattggttt actacagggt tgagttgacc cacaaacact ttcaggaaac ggtacagact
    10261 tcctgaataa atcaaatagt cacctgcgga aaaggaataa tcatcagatg tatgcccgcg
    10321 agtatcgctc aacacgcccg cataaagcga ttttctttca tctttcttgc ctcaccctta
    10381 tctgtagtgc gcaagtttat gcgaagccgg atatgcggcc actggggccg aatatagccg
    10441 ataaaggctc cgtgttttac catttcagcg tcacctcttt cgactctgtc gatggcacac
    10501 gccattatcg ggtatggacg gccgtgccga atacaaccgc accggcatcg ggttacccga
    10561 ttttatatat gcttgacggt aacgcagtta tggatcgcct ggatgacgaa ctgctcaaac
    10621 aattgtcaga aaaaacaccg ccagtgatcg tggctgtcgg gtatcagacc aacctccctt
    10681 tcgatctcaa cagcagggct tacgactata cgccagcagc agaaagcaga aaaacagatc
    10741 tccactcagg gcattttagc cgtaagagtg gtggcagcaa caacttccgc cagttactgg
    10801 aaacgcgtat tgccccaaaa gtggaacagg gactgaatat cgatcggcaa cgccgcggct
    10861 tatgggggca ctcctacggc ggcctcttcg tgctggattc ctggctgtcc tcctcttact
    10921 tccggtcgta ctacagcgcc agcccgtcgt tgggcagagg ttatgatgct ttgctaagcc
    10981 gcgttacggc ggttgagcct ctgcaattct gcgccaaaca cctggcgata atggaaggct
    11041 cggcgacaca gggtgataac cgggaaacgc atgctgtcgg ggtgctgtcg aaaattcata
    11101 ccaccctcac tatactgaaa gataaaggcg tcaatgccgt attttgggat ttccccaacc
    11161 taggacacgg gccgatgttc aatgcctcct ttcgccaggc actgttagat atcagtggtg
    11221 aaaacgcaaa ttacacagca ggttgtcatg agttaagcca ctaaacactg cccgctttta
    11281 cgcgggcagt acgcctgaaa cactacgatc agaatgatgc ggtaactccg gcatagtaag
    11341 cccggcctgg ctcgttatag gtattcgccc cttcagaaga tcggaagatc tgtttattga
    11401 ggatattact gacgccgaca ttaagacgca gatttttatt aatatcgtaa ttgaagttcg
    11461 tccccaccag tgaataagcg cccagctctt tacctgacag accgccagta tcttcactgc
    11521 gggtttccgc atgagtacgc ggtttttgtc tgccatataa cgtccagttg acgctggcag
    11581 aaaacgcctg ggtgatggtc cagttaagcg agttattgat agtatatttc gggatgaccg
    11641 acagaggatt accggtgtct ttttgctccg aagtgatcat ccatgtggca ttggtattcc
    11701 agttcagacg atctttcacc agtgggaaag acatactggc ttcgataccg tccaccagag
    11761 ctttcccgcc attctgccac ttgaggatat atgcgcctga agcggtttgc ccgataacgt
    11821 tatccccggc cacgatctta ttctggtaat cattgcggaa gtaggtcaca cttgcgtggt
    11881 aatcttccca ggtgaactcc agcccaattt ctttattgac gctgatttcc ggatcgagat
    11941 ctttattacc gatcaggtag cacccgcctg atgtaatatc ttttggacag ccattgcctt
    12001 tcgagtagag cagatagcct tcactggatt gatacaggtt tggggcttta aaggttcggg
    12061 caacccctgc tttgactttg aaataatcgc ccaattcctg cgaaagattc agactggggc
    12121 tgaagttccc gccggagtcg ctgagataat caaagcgcag gccgggaatg atattcgtgc
    12181 caggaaccgg ctcaatgtta tcttcaatat acagcgcact gatttgagaa tgatttttac
    12241 tgctgcgatc cgcagcagag ccagaaatac cgctgatatc actgtcattc accgtcaggc
    12301 tggtagagga aggatcatcg agcttatcgc ggttccactc tgcaccaacg gtcagcgttt
    12361 gatcaaccat cacattcaaa ggaatattaa gctcgccgct ggttcgccag gaactcaggc
    12421 gattggtcgt aaacttttca cccgctaaaa tacgcccttc accaccgccg gataatcctt
    12481 cattcatgcg ggtattattg gttttctcgt aataaacacc aaagcgactt tgtccccagt
    12541 cccagatacc attatgcgta atgccataat tctgtcggta caggcggttc gtctctttgc
    12601 cggattttgc caggctttcg gtaactgcac tggaagaact gttttgcgta tcgcccgcat
    12661 agatattccc ctggcggcta tatccggctt cgaaatcgag aatctgctgc ggatttaatt
    12721 tccacgagac aacgccgtta atatctttgt tacgtacccc ttcatgcccg gctgcgtttt
    12781 tcgtaccgac cggagaatta atatcccaac tgtcagcatc cgttttattc aggttaccat
    12841 acaaacgcgt ggtaagagca ttaccagcca gaggcccact aaggctgaaa ttggcgcgac
    12901 gcgtagcgcc ctcatcgcta ctttccggct gattggtgta taacgacagc gaaccgtgcc
    12961 agtcgttggt gggacgtttg gtaatgatgt tcaccacccc cccggctgcc cccgaaccgt
    13021 agcgcgccgc cgcagggccg cggatcactt caatacgctc aacctgttcc ggtggcaccc
    13081 agttggtgtc accgcgggta tcacgctctc cacgccagct ataacgcacg gagttacgtg
    13141 acgtcaccgg tacaccatca attaaaatta aggtgttttc cggccccata ccacgaatat
    13201 cgatctggcg gttgttaccg cgtgtgcccg aggcgctatt gccggtaaga ttgacgccag
    13261 gcattttacg aataatatct gaaaggtcgt ttaccggagg ggtcttttta atatcctcgc
    13321 tggtaataac cgacacgccc ggctgctgtt ttaatacctg ctcagcggtg gcttccacca
    13381 ccagagtctc gtcattatca tcgtcggagg atttggctac tgatacctgg ctattcaacc
    13441 caaccaggag cacagttagc gaccagagga ttttgttaat tctcatacct attccctaat
    13501 aaatgcctaa cttaaaatgt ttgatcgtta agctcacatc cttgccagat attttttact
    13561 gccattattg tttttatata agaatgataa ttaatatcat ttagcaaaaa aaaagcaatc
    13621 cctcacaaga taaatatatt gatttttcat aaatatcaaa ttgatatata acatatgttt
    13681 tttatttcat tgcactt
//
LOCUS       DAMZET010000113        13645 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.113, whole genome shotgun sequence.
ACCESSION   DAMZET010000113 DAMZET010000000
VERSION     DAMZET010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.113"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..233)
                     /locus_tag="POB34_003310"
     CDS             complement(<1..233)
                     /locus_tag="POB34_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HDI5920199.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQ"
     gene            369..1154
                     /gene="cmoM"
                     /locus_tag="POB34_003311"
     CDS             369..1154
                     /gene="cmoM"
                     /locus_tag="POB34_003311"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HDI5920200.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDHVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            1151..2473
                     /gene="mukF"
                     /locus_tag="POB34_003312"
     CDS             1151..2473
                     /gene="mukF"
                     /locus_tag="POB34_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HDI5920201.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            2454..3158
                     /gene="mukE"
                     /locus_tag="POB34_003313"
     CDS             2454..3158
                     /gene="mukE"
                     /locus_tag="POB34_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HDI5920202.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            3158..7618
                     /gene="mukB"
                     /locus_tag="POB34_003314"
     CDS             3158..7618
                     /gene="mukB"
                     /locus_tag="POB34_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HDI5920203.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            7879..9726
                     /gene="ldtD"
                     /locus_tag="POB34_003315"
     CDS             7879..9726
                     /gene="ldtD"
                     /locus_tag="POB34_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDI5920204.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAILAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQMTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            9907..10455
                     /locus_tag="POB34_003316"
     CDS             9907..10455
                     /locus_tag="POB34_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HDI5920205.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPPPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            10482..11129
                     /gene="gloC"
                     /locus_tag="POB34_003317"
     CDS             10482..11129
                     /gene="gloC"
                     /locus_tag="POB34_003317"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HDI5920206.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(11351..12541)
                     /gene="aspC"
                     /locus_tag="POB34_003318"
     CDS             complement(11351..12541)
                     /gene="aspC"
                     /locus_tag="POB34_003318"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HDI5920207.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(12726..>13645)
                     /gene="ompF"
                     /locus_tag="POB34_003319"
     CDS             complement(12726..>13645)
                     /gene="ompF"
                     /locus_tag="POB34_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HDI5920208.1"
                     /translation="GDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGN
                     KTRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVA
                     TYRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAAD
                     RTNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANK
                     TQDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYV
                     DYIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         3332 a         3413 c         3753 g         3147 t
ORIGIN      
        1 ttttgcgaat tattccatgt cggataggtg ctttcgatgg gacgcagcag gcggtcggct
       61 accggttgca aactcaataa caagagtgcc agccacccaa tgctaataaa aatcttcccg
      121 gttttttgaa aacggctaaa ccacagcagc gccaggccag cgccaataat taacaacatc
      181 agcggaagtg gcagcaacat attgccaatc acttttttca gtgtaaaaag catcctttct
      241 ggttcctttt ttaaccatat agcaagggat cgccagcgat attacaccag acgggttcat
      301 tctcgccgtg gctgtgacaa aatagcgtct ttatcagaaa acgtcttagc caaccggtgg
      361 agaaagcaat gcaggatcgc aatttcgatg atattgcgga aaagttttcc cgtaacattt
      421 acggcaccac caaagggcag cttcgacagg ctattctgtg gcaggatctc gatcacgtgc
      481 tggcggaaat gggcccgcaa aaactgcgtg tgctggatgc tggcggtgga gaagggcaga
      541 ccgcaatcaa aatggccgag cgtgggcatc aggtcatttt atgcgatctt tctgcgcaga
      601 tgatcgaccg cgcaaaacag gcggcagaag caaaaggtgt gagcgacaac atgcaattta
      661 tacattgcgc cgctcaggat gttgcttcgc atttggaaac gcccgttgat ctgatattgt
      721 tccatgcggt gctcgagtgg gtggccgatc cccgcagtgt attgcagacc ctgtggtcag
      781 tgttgcgtcc aggcggagtg ttgtcgttaa tgttctacaa tgcgcatggt ttgttgatgc
      841 ataacatggt cgccgggaat tttgattacg tgcaggcggg aatgccgaaa aagaaaaaac
      901 ggacgctttc gccagattat ccacgcgacc cggcgcaggt ttatctgtgg ctggaagaag
      961 ctggttggca aattatgggt aagacaggcg ttcgcgtgtt tcatgattat ctgcgcgaga
     1021 agcaccagca gcgcgactgc tatgaagcat tacttgaatt agaaacgcgt tattgccgtc
     1081 aggaaccgta tattaccctg gggcgttata ttcatgtcac cgcgcgcaaa ccgcagagca
     1141 aggataaagt atgagtgaat tttcccagac agtccccgaa ctggttgcct gggccagaaa
     1201 aaatgacttc tccatctcgc tgccggtaga ccgactctct tttctgctgg cggttgccac
     1261 gctgaacggc gagcgtctgg atggtgagat gagtgaaggc gagctggtgg atgcattccg
     1321 ccatgtgagt gatgcgtttg agcaaaccag cgaaaccatc ggcgtgcgcg ccaacaacgc
     1381 gatcaacgac atggtgcgtc aacgtctgct gaaccgcttt accagcgagc aggcggaagg
     1441 gaacgcaatt taccgtctga cgccgctcgg catcggcatc actgactatt acattcgtca
     1501 gcgcgagttt tctacgctgc gcctttctat gcagttgtcg attgtggcgg gtgagctcaa
     1561 acgcgcagca gatgccgccg aagagggcgg tgatgaattt cactggcacc gtaatgttta
     1621 tgctccactg aaatattcgg tagcagaaat tttcgacagt atcgacctga cgcaacgtct
     1681 gatggatgaa cagcagcagc aggtgaagga tgatatcgcc cagttgctga acaaagactg
     1741 gcgggcggcg atttccagct gtgaattgtt gctttcggaa acttccggaa cgctgcgtga
     1801 attgcaggat acgctggaag cggcaggcga caaattgcag gctaatctgt tgcgcattca
     1861 ggatgcgacg atgacccatg acgatctgca tttcgtcgat cgtctggtgt tcgatctgca
     1921 gagcaaactc gatcgtatta tcagttgggg ccagcaatcc atcgacttgt ggattggcta
     1981 cgaccgccac gtacacaaat ttattcgtac cgcgatcgat atggataaaa accgcgtctt
     2041 tgctcagcgg ttacgtcagt cggtacaaac ctattttgat gagccgtggg cgctaactta
     2101 tgccaatgcc gatcgtctgc tggatatgcg tgacgaagag atggcactgc gcgatgaaga
     2161 agtgactggg gaacttcctg aggatctgga atacgaagag tttaacgaga tccgcgaaca
     2221 gctggcggcg atcatcgaag aacaacttgc cgtgtacaaa accagacaag tgccgctgga
     2281 tcttggtctg gtggtacgcg aatatctgtc acagtatccg cgtgcacgtc actttgacgt
     2341 tgcgcgtatt gttattgatc aggcggtacg tcttggcgta gcgcaagcag atttcaccgg
     2401 actgccagcg aaatggcagc cgattaatga ttacggagcc aaggtacagg cgcatgtcat
     2461 cgacaaatat tgaacaagtg atgccggtta agctggcgca ggcgctggcg aatccgttat
     2521 ttccggcgct ggacagcgcc ttacgttcag gccgccatat tggcctcgac gaactggata
     2581 atcatgcatt cctgatggat tttcaggaat atctggaaga gttttacgcg cgttataacg
     2641 ttgagcttat tcgcgcacca gaagggttct tctatttacg cccacgttcc accacgctga
     2701 tccctcgttc cgtcttgtcg gaactggata tgatggtcgg gaaaatcctc tgttatctct
     2761 atctcagccc ggaacggctg gcgaatgagg ggattttcac ccagcaggaa ctgtacgacg
     2821 aactgctcac cctggccgat gaagcaaaac tgctgaaact ggtgaacaac cgttcaaccg
     2881 gttcagacgt tgaccgtcag aagttgcagg agaaagtacg ttcttcgctc aaccgtctgc
     2941 gtcgtttagg catggtgtgg tttatgggcc acgacagcag caagtttcgc attaccgaat
     3001 cggtgttccg cttcggagcc gatgtgcgtg ctggcgacga tccccgtgaa gcacagcgtc
     3061 gcctgattcg tgatggcgaa gcaatgccga ttgaaaatca tctgcaactc aacgatgaaa
     3121 ccgaagagaa tcagccagat agcggagagg aagaataatg attgaacgcg gtaaatttcg
     3181 ctcactgacg ctgattaact ggaacggctt ttttgcccga acttttgacc ttgacgagct
     3241 ggtcacgacg ctttctggcg gtaacggggc gggtaaatcc accaccatgg cggcgttcgt
     3301 tacggcgctg atccccgacc tgaccctgct gcatttccgt aacactacgg aagccggggc
     3361 caccagcggt tcgcgcgata aaggtctgca cggtaagctg aaagcgggtg tctgttattc
     3421 gatgctcgac accattaact cgcgccacca gcgcgtggtg gtcggtgtgc gtctgcaaca
     3481 ggttgccgga cgcgatcgta aagtggatat caaaccattt gccattcagg gactgccgat
     3541 gtcggtgcag ccgacacagc tggtgaccga aaccctgaac gaacgccagg cgcgcgtgct
     3601 gccgcttaac gagctgaaag acaagctcga ggcgatggaa ggcgtgcagt ttaaacagtt
     3661 caactccatt accgattacc actcgctgat gttcgatctt ggcatcatcg cgcgtcgtct
     3721 gcgctccgca tctgaccgta gcaaattcta tcgtctgatc gaagcttcgc tgtatggcgg
     3781 gatctccagt gccattaccc gttctctgcg cgactacctg ttgccggaaa acagcggcgt
     3841 gcgtaaagcg ttccaggaca tggaagcggc gctgcgtgaa aaccgcatga cgctggaagc
     3901 gattcgtgtc acccagtcgg accgtgacct gtttaagcat ctgatcagcg aagctaccaa
     3961 ctacgtggcg gcggactaca tgcgtcacgc caacgagcgc cgtgtccatc tcgacaaagc
     4021 cctggagttt cgtcgcgagt tacatacttc gcgtcagcaa ctggcggctg agcagtacaa
     4081 acacgtcgat atggcgcgtg agctggcaga gcacaacggt gccgaaggcg atctggaagc
     4141 ggattatcag gcggccagcg atcacctgaa cctggtgcaa accgcactgc gtcagcagga
     4201 gaagatcgaa cgctacgaag cggatctcga tgagctgcag atccgtctgg aagagcaaaa
     4261 cgaagtggtg gcagaagcca tcgaacgcca ggaagagaac gaggctcgcg cggaagctgc
     4321 cgaactggaa gtggacgagc tgaaaagcca gcttgctgac taccagcagg cgctggacgt
     4381 ccagcaaacg cgcgcgatcc agtataacca ggcgattgct gcgcttaatc gcgccaaaga
     4441 actgtgccat ctgccggact taaccgccga cagcgccgcc gaatggctgg aaaccttcca
     4501 ggcgaaagag ctggaagcga ctgaaaaaat gctctctctt gagcagaaaa tgagcatggc
     4561 gcaaaccgcg cacagccagt ttgagcaggc ttatcagctg gtggtggcaa tcaacggccc
     4621 gctggcgcgt aacgaggcgt gggatgtcgc tcgcgaacta ttgcgcgaag gggtcgatca
     4681 gcgtcacctg gcagagcagg ttcagccgtt gcggatgcgc ttaagcgaac tggaacagcg
     4741 tctgcgcgaa cagcaagaag ctgagcgtct gctggcagat ttctgcaaac gtcagggcaa
     4801 gaattttgac attgacgagc tggaagctct gcatcaggag ctggaagcgc gcattgcctc
     4861 tctttccgat agcgtgtcta acgcccgtga agagcgcatg gcactgcgcc aggaacagga
     4921 acagctgcag tctcgcattc agagtttgat gcagcgtgcg ccggtttggc tggcagcgca
     4981 aaacagtctc aaccagttga gcgaacagtg cggcgaagag tttacctcca gccaggacgt
     5041 caccgaatat ctgcaacagt tgctggagcg tgagcgagag gcgattgttg aacgcgatga
     5101 agtgggcgcg cgcaaaaacg ccgtcgatga agagatcgaa cgtttaagcc agcctggcgg
     5161 ctctgaagat cagcgtctga acgcgctggc ggagcgtttt ggtggtgtgc tgctgtcaga
     5221 aatttatgac gacgttagcc tggaagatgc gccgtacttc tcagcgctgt atggcccgtc
     5281 acgccacgcc atcgtggtgc cagatctgtc acaggtaact gaacacctgg aaggcttgac
     5341 cgattgcccg gaagatctct atctgatcga aggagatccg cagtcattcg atgacagcgt
     5401 gttcagcgtt gatgagctgg aaaaagcggt agtggtgaaa atcgccgatc gtcagtggcg
     5461 ttattcacgt ttcccggaag tgccgctgtt tggtcgtgct gcgcgtgaaa gccgtattga
     5521 aagcctccat gccgagcgtg aagtgctttc cgaacgcttc gccacgctct cctttgatgt
     5581 acagaaaact cagcgtctgc atcaggcgtt cagccgcttt atcggcagtc atctggcggt
     5641 tgcgtttgag tctgacccgg aagcagaaat ccgtcaactg aacagccgtc gcgtcgaact
     5701 ggagcgggcg ttgagtaatc atgaaaatga taaccagcag cagcgtattc agtttgagca
     5761 ggcgaaagaa ggcgttacgg cgctgaaccg cattctgccg cgtctcaacc tgttggctga
     5821 tgacagcctg gcggatcgcg tcgatgaaat ccgcgaacgt ctggatgaag cccaggaagc
     5881 cgcgcgtttt gttcagcagt ttggcaatca actggcgaaa ctggaaccga tcgtttcggt
     5941 attgcagagc gacccggaac agttcgaaca gctaaaagaa gattacgcgt actctcagca
     6001 gatgcagcgc gatgcccgtc agcaggcgtt tgccctgacg gaagtggtgc agcgtcgtgc
     6061 gcactttagc tattctgact cggcagaaat gctgagcggt aatagcgatc tcaacgaaaa
     6121 gctgcgtgaa cgtctggaac aggcggaagc ggagcgtact cgcgctcgtg aagcgttgcg
     6181 tggtcacgca gcgcagttga gtcagtacaa ccaggtgctg gcttcgctga aaagttctta
     6241 cgacaccaaa aaagagctac tcaacgatct gcaacgtgaa ttgcaggata tcggcgtgcg
     6301 tgctgatagc ggggcagaag agcgggcgcg tattcgccgt gacgagctgc atgcgcaact
     6361 gagcaataac cgttcacgcc gcaatcaact ggaaaaagcg cttaccttct gcgaagcgga
     6421 gatggacaac ctgacccgca aactgcgcaa gctggagcgg gattactttg agatgcgcga
     6481 gcaggtagtg accgccaaag cgggctggtg tgcagtgatg cgcatggtga aagataacgg
     6541 cgttgaacgg cgcttacacc gtcgcgagct ggcttatctc tccgctgatg atttgcgctc
     6601 tatgtcggat aaggcattgg gtgcgctgcg tctggcggtg gcggataacg aacatctgcg
     6661 cgacgtgctg cgcatgtcgg aagatccaaa acgtccggaa cgtaaaattc agttcttcgt
     6721 ggcggtttat cagcatctgc gtgaacgtat tcgtcaggat attattcgta ccgatgatcc
     6781 ggtggaagcc atcgagcaga tggaaatcga acttagccga ctgaccgagg agttaacctc
     6841 ccgcgaacag aaactggcga tcagttcccg cagcgtggcg aacatcattc gcaaaaccat
     6901 tcagcgcgag cagaaccgta tccgtatgct caaccagggg ttgcagaacg tatcgtttgg
     6961 tcaggtgaac agcgtacgtc tgaacgtgaa cgtgcgtgaa acgcacgcca tgctgctgga
     7021 tgtgctctct gaacagcacg agcagcatca ggatctgttt aacagcaacc gtctgacctt
     7081 ctcggaagcg ctggcgaaat tgtatcagcg tcttaatccg cagattgata tggggcagcg
     7141 cacgccgcag accattggcg aagaactgct ggattaccgc aactatctgg aaatggaagt
     7201 tgaggttaat cgtggttccg atggctggtt gcgcgcagag tctggtgcat tgtcgaccgg
     7261 tgaggcgatt ggtaccggta tgtcgattct ggtgatggtg gtacaaagct gggaagatga
     7321 atctcgccgc ctgcgcggta aagatatctc tccttgccgc ctgctgttcc tcgatgaagc
     7381 agcgcgactg gatgctcgtt ctatcgccac gctgtttgaa ttgtgtgaac gtttgcaaat
     7441 gcagctcatc atcgcagcgc cggaaaatat cagcccggag aaaggcacca cctataaact
     7501 ggtgcgtaaa gtcttccaga ataccgaaca cgttcacgtc gtcggcctgc gaggatttgc
     7561 gccgcaactc cctgaaacgc ttccaggaag tgacgaagcg ccttctcagg cgagttaaaa
     7621 ttaaggcggc agcaatgccg ccttttcttt ttccgaaaac tccgtttctg cactaaaaaa
     7681 gtggcacatt acggcgcgct tttctttaaa cttctttaca ttaggttatg taaaaacgta
     7741 tcggcgttta tatactgaag ataagcctga tgagtaacag gcttgctcgt catactttcg
     7801 tgagtattgg cgttgtacag gcaagtcgta aaataacagc ctggctattc agagtatgat
     7861 aaaaacaggg ggcaagggat gttgcttaat atgatgtgtg gtcgtcggct gtcggcaatc
     7921 agtttgtgcc tggccgtaac attcgctcca ctgttcaatg cgcaggccga tgagcctgaa
     7981 gtaatccctg gcgacagccc ggtggctgtc agtgaacagg gcgaggcact gccgcaggcg
     8041 caagccacgg caatattggc ggggatccag ccattgcctg aaggtgcggc agaaaaagcc
     8101 cgcactcaaa tcgaatctca attacccgca ggttacaagc cggtttatct taaccagctt
     8161 caactgttgt atgccgcacg cgatatgcaa cccatgtggg aaaaccgtga tgctgttaaa
     8221 gccttccagc aacagctggc agaggtggcg attgccggtt tccagccgca gtttaataaa
     8281 tgggtagagt tactgaccga tcctggtgtt aacgggatgg cacgcgacgt agtgctctct
     8341 gatgcgatga tgggctatct ccatttcatt gcaaatattc cggtcaaagg cactcgctgg
     8401 ctatatagca gtaaacctta tgcacttgca acgccgccgc tctcggtgat taaccaatgg
     8461 cagctggcgc tggataaagg tcaattgcct acgtttgttg caggactggc accgcagcat
     8521 ccgcaatatg cggcgatgca tgaatcgtta ctggccttac tcagtgacac caaaccgtgg
     8581 ccccaactga ccggcaaagc aacgttgcgc ccagggcagt ggagtaacga cgtaccggcg
     8641 ttgcgcgaaa tattgcaacg cacaggcatg ttggacgggg ggccgaaaat tactctacct
     8701 ggcgatgaca cgccaactga cgcggtagtc agcccatccg ctgttactgt tgaaacagca
     8761 gaaactaagc cgatggacaa gcaaatgacg tctcgtagta aacctgcgcc tgccgttcgt
     8821 gccgcctacg ataatgaact ggtggaagcc gttaaacgtt ttcaggcatg gcaaggattg
     8881 ggggcagatg gtgctattgg cccggcaaca cgtgactggt taaacgtaac gcccgcccag
     8941 cgtgctggcg tgttggctct caacatccag cgattgcgct tgctgccaac agagctttct
     9001 accgggatca tggttaacat tccggcctat tcgctggtct actatcagaa cggcaatcag
     9061 gtgctggatt cgcgagtcat tgtcggtcgc cccgatcgca aaacgccgat gatgagcagt
     9121 gcccttaaca acgtagtggt aaacccgccg tggaacgtac ctccaacact ggcacgcaaa
     9181 gatattctgc caaaagtgcg caacgatccg ggatatctcg aaagccatgg ctatacggtg
     9241 atgcgcggct ggaacagcag agaagcgatt gacccatggc aggttgactg gtctacaatc
     9301 acggcctcga atttaccgtt ccgcttccag caggctccag gcccacggaa ctcgctgggg
     9361 cgctataaat tcaatatgcc gagttcagag gccatttatt tgcatgacac gccgaaccac
     9421 aatctgttca agcgtgatac acgcgcattg agctcaggct gtgtacgagt gaataaagct
     9481 tccgatctgg cgaatatgct gttgcaggat gcaggctgga atgacaaacg tatttctgat
     9541 gcgctgaagc agggtgatac acgttacgtc aatattcggc agtcgattcc ggtgaatctc
     9601 tactacctga cggcctttgt tggtgcagat ggtcgtaccc agtatcgtac agatatttac
     9661 aattatgatc tgcctgcgcg atccagctcg caaatcgtat cgaaagcgga acaattaatc
     9721 aggtaaatga agtagttcgg ggaaatcagt tgtcgtaata attagcatga ttgggggcga
     9781 ttctctgcag cccccgtcac tgctggggtt gagtcatctt gacgtctgct ttacgggcgg
     9841 ttaaggtgcc tcttgtgcgc cagaagtgca tataaacgat aacattgacc tgtagacttg
     9901 attatcatgg acaaattcga cgctaatcgc cgcaaattgc tggcgcttgg tggcgttgca
     9961 ctcggtgccg ccatcctgcc gccccctgcg tttgcaacac tctctacccc acgcccgcgc
    10021 attttgacac tcaataatct tcataccgga gagtcaatca aagcggagtt tttcgatggc
    10081 agaggctata ttcaggaaga attggcaaaa cttaaccatt ttttccgcga ttaccgcgcg
    10141 aacaaaataa agtccatcga cccaggatta ttcgaccagt tgtatcgcct gcaagggttg
    10201 ttaggcacgc gcaaaccggt gcaactcatt tccggttatc gttctattga taccaacaat
    10261 gaactacgcg cccgcagccg tggagtagcg aagaaaagct atcacactaa aggccaggcg
    10321 atggatttcc atattgaagg tatcgcgtta agcaatattc gcaaagccgc gttatctatg
    10381 cgcgcaggtg gtgtaggata ttatccacgt agtaactttg tgcatattga taccgggcca
    10441 gcacggcact ggtagtaatt gcttaacgaa acaggggcag tatgaactat cgtattattc
    10501 cggtcaccgc attctcccag aactgttcat taatctggtg tgaacaaacc cgtctggccg
    10561 cactggtcga tcctggcggc gatgcggaaa aaatcaaaca ggaagttgat gacagcggcc
    10621 tgacactgat gcagatcctg ctgacgcatg gtcatctgga ccacgttggc gcagcggcgg
    10681 aactggcgca acattacggc gtgccggttt ttggcccgga aaaagaagat gagttctggc
    10741 tgcaaggctt gcctgcgcaa agtcgtatgt ttggtctgga agagtgccag ccgctgacgc
    10801 cagatcgttg gctgaacgaa ggcgatacca tcagcatagg gaatgtgact ttacaggtgt
    10861 tacattgccc tgggcatacg ccgggtcatg tcgtgttttt tgatgatcgg gcaaagctgc
    10921 tgatttctgg cgatgttatt ttcaaaggcg gagtagggcg cagtgacttc ccgcgtggcg
    10981 atcataatca actgatttct tcaatcaaag ataaattgct gccactgggg gatgacgtga
    11041 catttattcc gggtcacgga ccattatcca cacttggtta tgaacgcctg cataatccct
    11101 tcctgcaaga cgaaatgccc gtctggtaag gcacataaaa aagcccgctt ttaatgcggg
    11161 cctggatttc tggcaaagtg cgctttgttt atgccggatg cggcacgagc gccttatccg
    11221 gcctacaaaa tcgtgcaaat tcaaaatatt gcaggggacg tgtaggcctg ataagcgtag
    11281 cgcatcaggc aatgttgcgt ttgtcatcag tctcagcccg cttttcagcg ggcttcattg
    11341 tttttaatgc ttacagcact gccacaatcg cttcgcacag cggagccatg ttatctggtg
    11401 tcatcccggc cacatttacg cgaccagaag caaccgcata tacgccaaac tcttcgcgca
    11461 gacgcagcac ttgttctttt gtcagaccac tgaaggagaa catgccgttc tgtttgatga
    11521 taaagctgaa gtcgcggttt gcgccttttt cctgcagcgt attgacgaac aactgacgca
    11581 tacgctgaat acgctggcgc atatcagtca gctcttgttc ccaaatcgca cgtaacgcat
    11641 cgttgctcag gatggtggca acaacagaag cgccgtgtgc tggtgggtta gagtagttag
    11701 cgcgaatcgc cgctttcatt tggctgaatg cgcgatcaac ggtttcactg tcggcagcaa
    11761 ccagagtaca agcgccaaca cgctcgttgt acaggccaaa gtttttagag taggaactgg
    11821 caacaatcag ctctttatgc atagccgcga aagcgcgcag tccttcagca tcttcttcca
    11881 gaccacgggc aaaaccctgg taagcgaagt caaacagcgg taaccagcct ttctcaactg
    11941 agagttgtgc cagtgtctgc cattgttcca gcgtagggtc gataccggtt gggttatggc
    12001 agcagccatg gaacagcact acgtcgccag cctgagcttc gttcaggctg ttaatcagtg
    12061 catcgaagtc aagagtgtga ttttccgcat cataataagc gtattcacga acttccagac
    12121 ctgcagagtt aaagacgctc ttatggttcg gccagcttgg gttgctcacc cacactcgct
    12181 taacgctggt attttttgcc aggaaatcgg cagctacgcg tagtgcgcca gtgccccccg
    12241 gagtctgtgc cgtgcgagca cgtttgtcat tgatcagggc gctaccttta ccaaacagca
    12301 gttcctgagt gcagcgacca aattcaggga tgccgtcaat gccgaggtaa tttttggtgg
    12361 tttcattttc gagcagatac tgttcagcct ttttcacgct ggtcagtacc ggggttttgc
    12421 ccgtctcatc tttatagaca ccaatcccga ggttaatttt gccgggacgt tcatcggcac
    12481 gaaacagatc ggccaggccc agaatcgggt cggcaggagc ggcggtaatg ttctcaaaca
    12541 tgacgaggtt ccattatggt tacagaaggg aagtccgcta tcagggtaac gggagattta
    12601 caaaattcca actattactg atgaaaacgc aggctgtttt tgcaagacgt gagattgctc
    12661 tggaaggtat aaaaaaaaca ggaccaaagt cctgtttttt cggcatttaa caaagaggtg
    12721 tgctattaga actggtaaac gatacccaca gcaacggtgt cgtctgaacc tacgcccagt
    12781 ttgttgtcag aatcgatctg gttgatgatg tagtcaacat aggtggacat gtttttgttg
    12841 aagtagtagg ttgcgcccac ttcaaagtag ttcaccagat caacatcacc gataccttct
    12901 acgtctttcg ctttagattt ggtgtaagcg atggacggac gcagaccgaa atcgaactgg
    12961 tattgcgcaa ctaacagaac gtcttgcgtt ttgttggcga agccgctggt gtttgtaaat
    13021 ttattagtga tcggcgtagc gttacgggtt tcaccgtagt tcgctgccag gtagatgttg
    13081 ttcgcgtcgt acttcagacc agtagcccac tgttcagctt ttttaccgtt gccaagaggt
    13141 tgagcttctt gcaggttggt acggtcagct gcaccataag caccaacgat accaaagcct
    13201 tcgtattcgt agctgataga accgccaaca ccgtcgccgt tagaacggcg tgcagtgtca
    13261 cgctcgtttt tacccaggta ctgaacagcg aagttcaggc catcaaccag accaaagaag
    13321 ttggagttac gataggtagc aacgccgcca acacgaccaa cgaagaagtc atcgctgtat
    13381 gcagtatcac caccaaattc tggcagcata tcggtgtaac ccagtgcatc ataaaccaca
    13441 ccgtagttac ggccgtaatc gaaagaacca acgtcagcgt atttaagacc cgcgaatgcc
    13501 agacgcgttt tgttaccagt ttgagcgtca gcgccttcag agttgttacc ctggaagtta
    13561 tattcccact gaccataacc ggtcagatcg gaattgattt gagtttcccc tttaaaacca
    13621 agacgggcat aggtcatgtc gccat
//
LOCUS       DAMZET010000114        13551 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.114, whole genome shotgun sequence.
ACCESSION   DAMZET010000114 DAMZET010000000
VERSION     DAMZET010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.114"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..576)
                     /locus_tag="POB34_003320"
     CDS             complement(<1..576)
                     /locus_tag="POB34_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDI5920209.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIE"
     regulatory      complement(564..708)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            973..1881
                     /gene="yvcK"
                     /locus_tag="POB34_003321"
     CDS             973..1881
                     /gene="yvcK"
                     /locus_tag="POB34_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HDI5920210.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(2073..4094)
                     /gene="uvrB"
                     /locus_tag="POB34_003322"
     CDS             complement(2073..4094)
                     /gene="uvrB"
                     /locus_tag="POB34_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HDI5920211.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(4673..5350)
                     /gene="bioD"
                     /locus_tag="POB34_003323"
     CDS             complement(4673..5350)
                     /gene="bioD"
                     /locus_tag="POB34_003323"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDI5920212.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(5343..6098)
                     /gene="bioC"
                     /locus_tag="POB34_003324"
     CDS             complement(5343..6098)
                     /gene="bioC"
                     /locus_tag="POB34_003324"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HDI5920213.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPKGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(6085..7239)
                     /gene="bioF"
                     /locus_tag="POB34_003325"
     CDS             complement(6085..7239)
                     /gene="bioF"
                     /locus_tag="POB34_003325"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HDI5920214.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIAADRLSHASLLEAASLSPSQLRRFAHNDVTHLAR
                     LLASPCPGQQMVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(7236..8276)
                     /gene="bioB"
                     /locus_tag="POB34_003326"
     CDS             complement(7236..8276)
                     /gene="bioB"
                     /locus_tag="POB34_003326"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDI5920215.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            8363..9652
                     /gene="bioA"
                     /locus_tag="POB34_003327"
     CDS             8363..9652
                     /gene="bioA"
                     /locus_tag="POB34_003327"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDI5920216.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGKR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            9711..10187
                     /locus_tag="POB34_003328"
     CDS             9711..10187
                     /locus_tag="POB34_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HDI5920217.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(10586..10732)
                     /locus_tag="POB34_003329"
                     /pseudo
     CDS             complement(10586..10732)
                     /locus_tag="POB34_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            10796..11248
                     /locus_tag="POB34_003330"
                     /pseudo
     CDS             10796..11248
                     /locus_tag="POB34_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(11290..12033)
                     /locus_tag="POB34_003331"
     CDS             complement(11290..12033)
                     /locus_tag="POB34_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDI5920218.1"
                     /translation="MATTCSVILILESFDVYFGKESVFLERGSSVLVDSSSRDFFLTY
                     PERVIVADFGAEFISRYLKANNLRDISDCREYPSYLKINFADFSLIKGLISWANHCAE
                     YIEIFDESIAFTCLSAFSSEKQFGVFLFECLKSTGAKVKTIIHTDLSAPWRLKDISSR
                     LYLSESLLKRKLKEEGVSFSKIILDERMQMAEYLLSTRCYPISKVAKVCGYASVSYFT
                     YVFRRYFGVSPSQYSQSSSESKILTHQGI"
BASE COUNT         3403 a         3443 c         3273 g         3432 t
ORIGIN      
        1 ttcgatgaaa cgcagctgga tagggcgatg ctggatccag ttcagaaagg tgtcgagctg
       61 gtgatgatta acatcacgca tcagcacggt attgactttg accttctcaa aaccggcctc
      121 aaatgcagca tcaatccccg ccatgacctg gttgaattta tcctgcccgg taatagcgtg
      181 aaactggcgg gcgtccagac tgtcgacact gacgttaatg ccagtaagtc ccgcatcgcg
      241 ccagttcgcc acatcgcgtt ccagacggta accattggtg gtgaccgcaa tctggcggat
      301 agcgtcgttt tcccgcacag cggcgatgat atcggtaaag tcgcggcgta aagacggctc
      361 accgcctgtc aggcgcactt tttcggtgcc cagactggcg aaggcgcgcg taacccggcg
      421 aatttcatcg acggtaagaa agcctttatt ggtgacgccg ctcggtttgt agccatccgg
      481 caggcagtag gtgcaacgaa agttacacac atcggtaatc gacaggcgca agtagtaaaa
      541 cttacgcgca aatgcatcag tcagttgtga agccatgtac acctttccag atacgggagg
      601 cgaagtcatt tcttccttcg ccctggtggc aatctttacg agcattgtca cggccaaagc
      661 accgtatcag ttgacccagg tgcagaggct agagtgttta gtggttatgc cgatactagc
      721 gtgtaaatga gatttttacc atccacactt tcgctatgta atcatgtata tagcgtcatg
      781 atcgtgctat ttcgctacag aataagggaa aatggcggct ttactttgat atacatcatc
      841 agggaggcat agcgtgcatc gtcctttaga ggtagaagaa aatagtttgt atcctcaata
      901 ttggcaggtt aattgctgtt tccccgcaat ttgcgctact gtagcgcgga taatctgact
      961 ccaggaaact atatgcgcaa tcgtacgctg gctgatcttg atcgtgtcgt tgctctcggc
     1021 ggagggcatg gactgggacg cgttctctca tcactttcgt ctttgggttc tcgtttaacg
     1081 ggtatcgtca ccaccaccga taatggtggc tcgacggggc gtattcgccg ttcagaaggc
     1141 ggcattgcct ggggcgatat gcgcaactgc ctcaaccagc tgataacgga accgagcgtc
     1201 gcctccgcga tgtttgaata ccgttttggt ggcaatggcg aactttccgg tcataatctc
     1261 ggaaacttga tgttaaaggc gctggatcac cttagcgtgc ggcctctgga agccatcaat
     1321 ttaattcgta atctgctgaa agtggatacg catttgattc caatgtcaga gcatcctgtt
     1381 gatctgatgg cgattgacga tcaggggcat gaagtttacg gcgaggtcaa tatcgaccag
     1441 ttaactacgc cgattcaaga gttattgtta acgcctaatg tacccgcaac gcgtgaggcg
     1501 gttcacgcta tcaatgaagc ggatctcatc attattgggc ctggcagttt ttataccagc
     1561 ctgatgccaa ttctgctgct gaaggaaatc gcccaggcat tacgccgcac gccagcgccg
     1621 atggtttata tcggcaatct ggggcgtgag ttgagtttac ctgcggctaa tttgaagctg
     1681 gaaagcaagc tggcaattat ggagcagtat gttggtaaaa aggtcattga tgcggtcatc
     1741 gtcgggccaa aagtggatgt ctcggcggtg aaagagcgga ttgtgatcca ggaggtactg
     1801 gaggccagcg atattccgta tcgtcatgac cgccagttgt tacataacgc gctggaaaag
     1861 gcgttacagg ctttaggtta acggaaatcc gaaggaaaat tccggcttcc tattgaagac
     1921 aaagtgcgcg ttgtttatgc cggatgcggc gtgaacgcct tatccggcct acaaaccgcg
     1981 caaattcaat atattgcgga gaaaatgtag gcctgataag cgtagcgcat caggctgttt
     2041 tccgtttgtc atcagtcttc ttcgctatcc tgttacgatg ccgcgataaa cagctcacgc
     2101 agctgatgca actggtcacg aatttgcgcc gcttcttcga actccagatt ctgcgcgtgt
     2161 tgcatcatca acccttccag ctcatggatt ttctgctgca acgctttagg cgacatatcc
     2221 atcggcacat tatccggctc aacaatcggt cgcgattttc ctctgccctt cgctttggtt
     2281 ttagcaatgt tctgccccaa cgccaggata tcgaccactt tcttgttcaa gccttgcggc
     2341 gtaatgccgt gttcctcgtt gtacttctgc tgtttctcgc ggcgacgttc ggtttcgcca
     2401 atcgctttcg ccattgatgg ggtgatctta tcgccgtaga gaatcgcttt accgttaacg
     2461 ttacgtgccg cacgaccaat ggtctggatc aacgaacgtt cggaacgcag gaagccttct
     2521 ttgtcagcgt cgaggatcgc caccagcgaa acttccggca tatccagacc ttcacgcagt
     2581 aagttgatcc ctaccaatac gtcgaactca cccagacgca agtcgcggat aatctccata
     2641 cgctcgacgg tgtcgatatc tgagtgaaga taacgcacgc gctcaccgtg ttcttcgaga
     2701 tattcggtaa gatcttccgc cattcgcttg gtcagtgtgg tgaccagtac gcgttcgtta
     2761 attgccgctc gctgacgaat ctccgaaaga agatcatcaa cctgtgtcgc caccggccgc
     2821 acttcgataa tcgggtcaag caatccggtt ggacgcacca cctgatccac cacatcgccg
     2881 ccggattttt ccagctcgta attacccggc gtcgccgaaa catagatggt ttgcggcgct
     2941 aatgcttcga actcttcaaa cttaagcgga cggttatcca gcgctgatgg caggcggaag
     3001 ccgtactcca ccagtgtctc tttacgcgcc cggtcaccgc gatacatgcc gccaatttgt
     3061 ggaatggtga cgtgagattc atcgaccacc agcagcccat cagcagggag gtaatcaaac
     3121 agcgtcggcg gtggctcacc cggtccacga ccggagagga agcgcgagta gttttcaatc
     3181 cccgaacagt agcccagctc gttcatcatc tccagatcaa actgggtacg ctgggtcagc
     3241 cgctgctctt ccagcagttt gttgttttcc aacagcactt tgcgtctggc ggccagctct
     3301 tctttgatct cctccatcgc ctgtacgatg cgctcgcgcg gtgtgacgta gtgcgttttc
     3361 gggtagatgg taaaacgtgg aatagtggaa acaatctgcc cggtcagcgg gtcaaataac
     3421 gacaatcgtt ccacttcctc gtcaaacagt tccacgcgaa gtgcaatgtc atccgattct
     3481 gccgggaaga tatctatcac ctcgccacga acgcggaaag taccacgctg gaatgcttga
     3541 tcattacgag cgtattgcag ctccgccagt cggcgcagaa tcgcgcgctg atcgataatc
     3601 ataccgaccg tgagatggag catcatcttg agatataaat caggatcgcc cagaccataa
     3661 atcgcggaaa cagacgccac cacaaccaca tcacgccgct ccagcatcgc tttggtggcg
     3721 gacaaacgca tctgctcaat atgttcgtta accgaggcat ctttctcaat gaaagtgtcg
     3781 gaactcggta cataggcttc cggctgatag tagtcgtagt aggaaacgaa atattccacc
     3841 gcgttttccg ggaagaactc tttcatttcg ccatacagct gggccgccag cgttttgttg
     3901 ggcgcaagta ccatggttgg gcgctgaagg tcagcaatga cattggcaat ggtgaaggtt
     3961 ttcccggagc cagtcacgcc aagtaacgtc tggtgcgcca ggccatcttc cagcccctct
     4021 tcgagacgtc gaatcgcctc tggctgatcg ccagaaggtt taaaagcgga attcagtttg
     4081 aacggtttac tcatgagtcg ctacctgaag gagttgggcg ggcaggtatg taattttact
     4141 cgtcgtactt aattatgcca acaaattata ctggataaaa aaacagttca tcaccataat
     4201 atttctgtta cagcgtaaac tccgctgtca atcatgagca aaatttactc tgtggcgaga
     4261 taaaactccg gccttaccgg gttatcccca aagcaacggc ttttttaaca tttgtcaaga
     4321 tgagtgatga cagttttatg gcaagagatg cctgttcagt gactgccatt gcatttatct
     4381 aaccagttaa aaaataaaag atatttcttt gagccgtctt taacgccaat tcgcgtgcaa
     4441 gccgcgtatt ctctcgcttg cctcgtgttt tctaactcta atacacatgg ttatccacag
     4501 gaatagtgga taactgtctc cagcccctat cccccgccgc ctgggcaatt cccacattcg
     4561 ccgcatgagg cggaagtaaa atttttttcg tgattatttt agaatttaat tggttaaatt
     4621 gcagtcaatc gaagacgcga tctcgctcgc aatttaacca aatacaggat agctacaaca
     4681 aggcaaggtt tatgtacttt ccggttgccg cattttctgg attttctgca agccagggga
     4741 tctctcccag cagcggcgcg ggaatcatgc gggtgagcgt ggtcatatat tcagcgtgac
     4801 gttttcccgg aggcgtaaca tcgttcgcca cccaacccgc cagagtcagt ccggcgtgtt
     4861 gtattgcctg cgcagtcaac atcgcgtgat taatacagcc gagtttcaca ccaactacca
     4921 gtatcaccgg cagttgttcc tgtgttaccc aatctgcaaa agtgaaagtg tcagaaagcg
     4981 gcgtaaacca gccgccagca ccttccacta acacccagtc agcctgttgt tcaagcgcgc
     5041 gtaatccggc gctcattacc aatgattcta tcggcctgcc ctcttgcgcg ctgatgatgt
     5101 gcggcgaggt gggttctgcg aaggtgtaag gatttaccgt tgcgtaatcc agctgcaggc
     5161 tgctgttgtg ctgtaacgcc agcgcgtcac tattacgtaa gccttccggg gtcttttcgc
     5221 tgccagaggc gaccggttta taacctgccg tccgataacc tactgccttc gcggcttgta
     5281 aaagtgcaca actggcgacg gttttcccca cttcggtatc cgttccggtg acaaaataac
     5341 gtttactcac gagcaatcac tcccaaaaaa agatgatacg tcagaggata tcgcccctgt
     5401 tgttgcggcc aggccagttg caatcgctgc aattgcgaac gcgttaatat tcgcgggtcg
     5461 cgcccttcat gaagatgcgt agcaccgatg cctttcagcg aacgcatggc actgagcgca
     5521 tcatcaaacc acagcgtgat gggctgaata tgatgttgat aatgcacgcc gttcagcgac
     5581 tgttcgattt catctggcgg taaaaagcga ttagcatgcg gacgctcgtc caccgcctgc
     5641 cacgcctgat gcagttcggg taacgatccc tgcaccagcg tggtaaacgc gaccacgcct
     5701 ttggggcgca ccacccgata cagctcgcgg agtgccgtgg ataaattacc gcaccactgc
     5761 actgcgagat tgctccatgc aagatcgaac gtcgcagtcg ctaacggcag ggattcgata
     5821 tctcccgcca gataatggtc tgcggcatcc ttctggcgtg cctgaacaag cattggcggc
     5881 gagagatcta aggccgtcac ctgcgcgtga cgttcccgcc agtggcggct catccagcca
     5941 ggtccacaac ccgcgtccag tacgtgggtg tatttacgct gtggaagcat tgccagtaag
     6001 gcgtcagcac tctggcgctg tagatctgca tgttgctcat agtgtgcggc tgcccgacca
     6061 aatgccgctg caatggcttg tttattaacc gttgccatgc agcacctcca gcagacggtc
     6121 gatatcctgc atttcatgcg cagcggttag cgttaagcgc agtcgcgcag taccagcggg
     6181 tacggttggc gggcgaatcg ccgtgaccca gcagccttgc tggcgcagtt tttctgccag
     6241 ttgtaacgca cggctgttat caccgacaat caatggctgg atggcgctgc atgaatcagc
     6301 aagcgtaaac ggcaaatcct gtactccggc acgaaaacgc gtaatgagtg ccgccagttt
     6361 ttcgcgccgt gcatcaccct catcactgcg aatgaccgcc agcgacgcac gtaatgcctg
     6421 cgcctgagcg ggcggcatac tggtgctgta gataaggtgg cgggcgaatt gcagcagata
     6481 atccgccacc gtactggagc aaagcaccgc tgccccgctg acgccaaatc ctttgccaaa
     6541 agtcactacc agcaattctg gttttacctt ttgcagccag cagctgccgc gcccctgctc
     6601 cccgataacg cccgtgccgt gggcatcatc gaccatcaac cagccattgt gctgttgcgt
     6661 tacctgctgg atttccgcca gtggcgcact atcgccgtcc atgctgaaca cgccttctgt
     6721 caccaccatt tgctgccccg gacaggggga agcaagcaat cgcgccaaat gagtgacatc
     6781 gttatgagca aaacggcgaa gctgcgacgg gcttaaactg gcagcttcca gcaatgaggc
     6841 atggctaagc cggtcggcag caatacggtc ctctttcgcc atcatcgcgg caataactgc
     6901 ctgattagcg gcgaaaccag agataaacag cagtgcccgc gaatagccaa gccactcggc
     6961 cagctcttct tccagtgcct gatgcaccac gctataaccg ctgacgtgac cggagccgcc
     7021 gctaccgatg ccaaattgct ccgccccctg ctgccaggca cggataattt gcggatgatg
     7081 gcttaaacct aaataatcgt tactggaaaa gttcagatac tggcgatcat ccgccaccag
     7141 ccagcgtccg gctccttgcg ccaccggata acggcgacgc agggcatcgg cagcacgccg
     7201 cgcatcgagc gccgcgttga ttttctcctg ccagctcata atgctgccgc gttgtaatat
     7261 tcgtcggtgt ccggggtcat cagcgcctgt tcaagacgtt gctgttgttc gttatcccct
     7321 gccagcacgg cagtttgctg cggatttagc cccagtttgc ggaacagttg caggtcttta
     7381 tcttcttccg gattcggcgt ggtcagcagt ttgcaaccgt agaaaatcga gtttgcgcct
     7441 gccataaagc acatcgcctg agtctgttcg ttcatctgct cgcgtccggc agaaaggcgc
     7501 acgtaagagg ttggcatcat gatccgcgcg accgcaatgg tgcgaataaa atcaaaggca
     7561 tcgacatcat cgttatcggc aagcggcgtg cctttcacct tcaccagcat gttgattggc
     7621 acgctttccg gcggcgtcgg caggtttgcc agttgcagca ataatccggc gcgatctttt
     7681 accgtttcgc ctaagcccac aatgccgcca gaacagacct tgatcccggc atcgcgcact
     7741 ttttccagcg tatcgaggcg ttcctgataa gtgcgtgtgg tgatgatatt gccgtaaaac
     7801 tccggcgagg tgtccaggtt gtggttgtag taatccagcc cggcgttcgc gaggcgctgc
     7861 gcctgagatt cactcaacgt gcccagcgtc atacacgcct ccagccccat cgcttttacc
     7921 ccctgcacca tttgttccag gtacggcata tcgcgttcgt ggggattctt ccacgccgcg
     7981 cccatacaga agcgcgtcga tcctgccgct ttcgctttgc gcgccgactc cagcacctgt
     8041 tcaacttcca tcaaccgctc ggcttccagc ccggttttgt agcgcgagct ttgcgggcag
     8101 tatttgcaat cttccggaca agctccggtc ttaatcgaca gcaacgtgct gacctgcacc
     8161 tgacgaggat cgaaatgctg gcgatgcacc tgctgcgctt caaacagcag atccagcaac
     8221 ggtttttcaa ataattctgt gacttgcgac aatgtccagc gtgggcggtg agccatgggg
     8281 cttctccaaa acgtgttttt tgttgttaat tcggtgtaga cttgtaaacc taaatctttt
     8341 caatttggtt tacaagtcga ttatgacaac ggacgatctt gcctttgacc aacgccatat
     8401 ctggcaccca tacacatcca tgacctcccc tctgccggtt tatccggtgg tgagcgccga
     8461 aggttgcgag ctgattttgt ctgacggcaa acgcctggtt gacggtatgt cgtcctggtg
     8521 ggcggcgatc cacggctaca atcacccgca gcttaatgcg gcgatgaagt cgcaaattga
     8581 tgccatgtcg catgtgatgt ttggcggtat cacccatgcg ccagccattg agctgtgccg
     8641 caaactggtg gcgatgacgc cgcaaccgct ggagtgcgtt tttctcgcgg actccggttc
     8701 cgtagcggtg gaagtggcga tgaaaatggc gttgcagtac tggcaagcca aaggcgaagc
     8761 gcgccagcgt tttctgacct tccgcaatgg ttatcatggc gatacctttg gcgcgatgtc
     8821 ggtgtgcgat ccggataact caatgcacag tctgtggaaa ggctacctgc cagaaaacct
     8881 gtttgctccc gccccgcaaa gccgcatgga tggcgaatgg gatgagcgcg atatggtggg
     8941 ctttgcccgc ctgatggcgg cgcatcgtca tgaaatcgcg gcggtgatca ttgagccgat
     9001 tgtccagggc gcaggcggga tgcgcatgta ccatccggaa tggttaaaac gaatccgcaa
     9061 aatgtgcgat cgcgaaggta tcttgctgat tgccgacgag atcgccaccg gatttggtcg
     9121 taccggcaaa ctgtttgcct gtgaatatgc agaaatcgcg ccggacattt tgtgcctcgg
     9181 taaagcctta accggcggca caatgaccct ttccgccaca cttaccacgc gcgaggttgc
     9241 agaaaccatc agtaacggcg aagccggctg ctttatgcat gggccaactt ttatgggcaa
     9301 tccgctggcc tgcgcggcag caaacgccag cctggcgatt atcgaatccg gcgaatggca
     9361 gcagcaggtg gcggctattg aagtgcagct gcgcgagcaa ctggcaccag cccgtgatgc
     9421 cgaaatggtt gccgatgtgc gcgtactggg ggcaatcggt gtggtcgaaa ccactcgtcc
     9481 ggtgaatatg gcggcgctgc aaaaattctt tgtcgaacag ggtgtctgga tccggccttt
     9541 tggcaaactg atttacctga tgccgcccta tattattctc ccgcaacagt tgcagcgtct
     9601 gaccgcagcg gttaaccgcg cggtacagga tgaaacattt ttttgccaat aacgggaagt
     9661 ccgcgtgagg gtttctggct acactttctg caaacaagaa aggagggttc atgaaactca
     9721 tcagtaacga tctgcgcgat ggcgataaat tgccgcatcg tcatgtcttt aacggcatgg
     9781 gttacgatgg cgataatatt tcaccgcatc tggcgtggga tgatgttcct gcgggaacga
     9841 aaagttttgt tgtcacctgc tacgacccgg atgcgccaac cggctccggc tggtggcact
     9901 gggtagttgt taacttaccc gctgataccc gcgtattacc gcaagggttt ggctctggtc
     9961 tggtagcaat gccagacggc gttttgcaga cgcgtaccga ctttggtaaa accgggtacg
    10021 atggcgcagc accgccgaaa ggcgaaactc atcgctacat ttttaccgtt cacgcgctgg
    10081 atatagaacg tattgatgtc gatgaaggtg ccagcggcgc gatggtcggg tttaacgttc
    10141 atttccactc tctggcaagc gcctcgatta ctgcgatgtt tagttaatca ctctgccaga
    10201 tggcgcaatg ccatctggta tcacttaaag gtattaaaaa caactttttg tctttttacc
    10261 ttcccgtttc gctcaagtta gtataaaaaa gttgaatgtg aaacggtaaa aacaattaat
    10321 atcaacgact tataattgat tcagtctaaa aaatagacta cataatgcta caaaacacaa
    10381 catatccagt cactatgaat caaccactta gatggtatta gtgacctgta acagagcatt
    10441 agcgcaaggt gatttttttg tcttcttgcg ctaatttttt gtcaacacac tgggttagct
    10501 gaatttacaa ccacgctcag tatttcgata agcgcagaga aatgatgcag tggtgggcgg
    10561 attggcttga tgaaaaagtg gagtaatccg gaccgtactt ctttatccag gcataaaggc
    10621 tgtgggtggt gatatcgaga cgtgttgcaa cgctggcaac agaataaccg cgatcaacaa
    10681 cctgtttgac tgcttcagtt ttaaactctt cgggataacg cttaccgctc atgggcacct
    10741 ctctttaagc catcttaaat gactctgagg tgtctgttaa acccgtggcg attcaaaact
    10801 gcgtgttacg ttcgccctgg actgttgcga tcgtgaagcc atagactggg ccgcaagcac
    10861 gggaggttac gataaagcga cagtgcagga tgtcatgtta ggagcgatag aaaaacgctt
    10921 tggcgataaa gtaccggaac agtccatcca gtggctgaca gataacggtt cagcgtatag
    10981 agcgcatgaa acatggcagt tcgccagaga gttgaatctg gagccctgca caacggcaat
    11041 cagcagtcct cagagtaatg gaatggcaga aaggttcgtg aaaacgatga aggaagacta
    11101 catcgcgttc atgccgaaac cgaatgtaag aacggcattg cataatcttg cagtggcgat
    11161 cgaacattac aatgaaaacc atccgcacag tgcgttgggt tatcgctctc cgcgagaata
    11221 tcgacgtcag cgggtaacgt taacttaaga tacaccccct gtctggaaat aagggggcaa
    11281 gaacaatgat cagattccct ggtgagtaag aattttactt tctgaactgc tctgagagta
    11341 ttgacttgga gaaacaccaa aataacgtct aaatacataa gtaaagtatg aaacactggc
    11401 ataaccacag accttagcta ctttactaat aggataacaa cgagtgctga gtaaatattc
    11461 agccatttgc atcctctcat caagtatgat cttactgaat gataccccct cttctttcaa
    11521 tttcctcttt agtaaacttt cgctgagata taatcttgat gatatatcct taagacgcca
    11581 tggtgcagat aaatccgtat gaataatcgt tttaacttta gcccctgtgc ttttcaaaca
    11641 ttcaaacaga aatactccaa attgtttttc agaagaaaat gcagagagac atgtaaaagc
    11701 aatagactca tcaaaaattt ctatgtattc agcacagtga ttagcccaac taattaatcc
    11761 tttaattaaa ctgaagtcag caaagtttat ttttaaataa gatggatatt ccctacaatc
    11821 agaaatatcc cttaagttat tagctttcaa atagcgacta ataaactcag cgccaaaatc
    11881 cgccactatc actctttcag gatatgtcag gaaaaaatct ctagagctag agtcgacaag
    11941 tacagatgaa cctctctcca gaaacacact ctcttttccg aaataaacat caaaggactc
    12001 caaaatcaat ataactgaac atgtagttgc catatcatcc acccaactta actgaaacca
    12061 ggatgaagta tatgcatata aagttcactt tgccaaactt cagtataaaa aaccacataa
    12121 aaaatagggt gtgataaaaa ataccgtaac aataaagcaa aggattataa attccgttac
    12181 agttacaaat gatactcaag aaacaattcc ttaggaaaaa cttatttaca gccaataagt
    12241 aagacactta tatgatatca agttttcata aaacataact aggctaaata gctgcgccta
    12301 ataccgctac acttttgcca gcccatgttt gcctccgggt attgacccct tctctacgca
    12361 acttcagttc ccaccaccaa ctttgcggca gctttgtagg atcaatgtct aaaagaataa
    12421 tggtgaccga taagaaaaca actgaataac tgcaaatttt cgctcaaaac cttcctgtca
    12481 gatccatagc gaatcaagtg ctgaatgtca cagtatcgaa cagaaaacag tgacgatcta
    12541 acccttcaag aatattctac gattgttctg tttaggaaaa gcaaggcggg aagtcgggag
    12601 ataagtcatt gataaagtgg cggagagagg gggatttgaa cccccggtag agttgcccct
    12661 actccggttt tcgagaccga tgatacagag tcgaaaaatc aaagagttaa aaatttttct
    12721 tggaataaat gtcttaaaat cgtacacgaa aaatcaatga attacataac attaaagaag
    12781 aatattccaa gccttcttga ggctaccacc tgttttcagg tgtcattcag acgtgatttg
    12841 ccccagattg ggaaagcacg cttttttctc agctatcgtc ctcttatttt gccgttacat
    12901 ttctttttgc actctaatga ttaatttaca ataataatat cggtaattat tgagtaataa
    12961 taaattggtt attgcaaatg taatttaatt ccatcttcgc gttatattcc aatctttaat
    13021 ctccgcatgg aacaagttgc atgcgtgact atattaagac aacttataac caccaaaaca
    13081 tttgctttgc aagcaaaaac attgaccatt gactctaaga aaattccgat ctattgttag
    13141 ggtcatcaaa gttacttaac agtattccga aatgaaaata caaagcaagc taattgtctt
    13201 attaaacatt atcattgcat ttatattatt tgagctttta gctcaaaaat ttggtttgag
    13261 ttcattattt taaagcaaga taaacaatat cgggcactct agtttttata attaacaatt
    13321 aaacgtccat aaaatgttat ttacttttct tcgctattgt catatcttaa tatagtggct
    13381 tacgccactc tactttcatc acattttgta cgtttatgta ctcgccatga agctctttca
    13441 tggcgatttt ttattcttaa tccaagaaca tactccctac ctatagcaaa atcacttttt
    13501 gaacaaaaga ataacgtatt taggtaatca ttacgaattt accctatagg t
//
LOCUS       DAMZET010000115        13258 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.115, whole genome shotgun sequence.
ACCESSION   DAMZET010000115 DAMZET010000000
VERSION     DAMZET010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.115"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(68..607)
                     /locus_tag="POB34_003332"
     CDS             complement(68..607)
                     /locus_tag="POB34_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDI5920219.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(834..2138)
                     /gene="ndh"
                     /locus_tag="POB34_003333"
     CDS             complement(834..2138)
                     /gene="ndh"
                     /locus_tag="POB34_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HDI5920220.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(2546..3088)
                     /locus_tag="POB34_003334"
     CDS             complement(2546..3088)
                     /locus_tag="POB34_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI5920221.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3111..4136)
                     /gene="nagZ"
                     /locus_tag="POB34_003335"
     CDS             complement(3111..4136)
                     /gene="nagZ"
                     /locus_tag="POB34_003335"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HDI5920222.1"
                     /translation="MGPVMLDVKGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDSRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4147..4971)
                     /gene="thiK"
                     /locus_tag="POB34_003336"
     CDS             complement(4147..4971)
                     /gene="thiK"
                     /locus_tag="POB34_003336"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HDI5920223.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(4952..5593)
                     /gene="lpoB"
                     /locus_tag="POB34_003337"
     CDS             complement(4952..5593)
                     /gene="lpoB"
                     /locus_tag="POB34_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HDI5920224.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(5607..5984)
                     /locus_tag="POB34_003338"
     CDS             complement(5607..5984)
                     /locus_tag="POB34_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HDI5920225.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPITVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(5987..6346)
                     /gene="hinT"
                     /locus_tag="POB34_003339"
     CDS             complement(5987..6346)
                     /gene="hinT"
                     /locus_tag="POB34_003339"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HDI5920226.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            6680..8869
                     /gene="fhuE"
                     /locus_tag="POB34_003340"
     CDS             6680..8869
                     /gene="fhuE"
                     /locus_tag="POB34_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HDI5920227.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGIYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGTPRNFSITSTYQF"
     gene            complement(8929..10362)
                     /gene="ptsG"
                     /locus_tag="POB34_003341"
     CDS             complement(8929..10362)
                     /gene="ptsG"
                     /locus_tag="POB34_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HDI5920228.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(10657..11454)
                     /locus_tag="POB34_003342"
     CDS             complement(10657..11454)
                     /locus_tag="POB34_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDI5920229.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(11465..12469)
                     /gene="holB"
                     /locus_tag="POB34_003343"
     CDS             complement(11465..12469)
                     /gene="holB"
                     /locus_tag="POB34_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDI5920230.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNMLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(12466..13107)
                     /locus_tag="POB34_003344"
     CDS             complement(12466..13107)
                     /locus_tag="POB34_003344"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDI5920231.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13097..>13258)
                     /locus_tag="POB34_003345"
     CDS             complement(13097..>13258)
                     /locus_tag="POB34_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDI5920232.1"
                     /translation="GADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYL
                     KVLKEKNAQ"
BASE COUNT         3316 a         3533 c         3242 g         3167 t
ORIGIN      
        1 aaataagtcg caaaaaaagc ccctcagcct gagcgaatga ggggccaaat tgcagagtac
       61 agccttgtta tactttgtta ttcaaaatca gttgcccatt actatctagc gggatctgcg
      121 tacccggatc gcggtccatg cggattttgc cctgctgatc gccaatctta taggtcacat
      181 cataaccgag cattttttct gacttgtcat acaccgtttt acaacgctgt tgcgtagtcg
      241 tgtaagtatc gctttcctgg agagagccct ggatctggtt accggcatat ccaccaccca
      301 gcgcccccac aacagtggcg acatctttac cacgaccacc accaaactga tgccctatca
      361 cgccgccagc aacagcgccg agcaccgacc cggtaatgcg attttcatcc tgcaccggtc
      421 gacgatgggt cactgtgacg ttgcgacact cctgacgcgg tgttttaacc gtttccttga
      481 ttggggttgc agaaacaacc tgagcgtatt gcgggccccg ttcaaacacg ttcagactgg
      541 ccactgccgc tacgcccagc gcagctgcga caccaatccc gatacccgcc aacattgatt
      601 tattcacggg aacacctcct tctttgcagt tatgccaatc atgcaagcga aggagaaaag
      661 cagctatcag acagaagatg aaaagcgcgg gaatggctgg aaatacttaa ggattcggag
      721 gagtctgaag gaatgcctga tgcgacgctt aacgcgtctt atcaggccta ccgctcgtac
      781 catttgtcca gggcggatga ggcgtttatg ccacatccgc cagtgtacgt cgattaatgc
      841 aacttcaaac gcggacggat aacgcggtta atactcccca ccagcatcat taatccggtt
      901 ttaaagtaac catgcagcgc aatctgatgc attcggtata gcgagatata tacaaagcgc
      961 gcaattcgtc cttcaatcat cattgagccg cgcgtcaggt tacccatcag gctaccgacg
     1021 gtggagaagt tcgacagcga taccagcgaa ccatgatctt tatactgata atttttcagc
     1081 ggcttaccgt tcatctgcgc cagtatgttg ttcattgcgc aagtcgccat ctggtgtgca
     1141 gcctgagcac gcggcggaac aaagccccct tccggacgcg ggcatgacgc gcagtcgcca
     1201 atagcgtaaa tgtctggatc gcgggtggtt tgcagcgtcg gttccaccac cagctggttg
     1261 atacggttag tttcaagacc accgatatct ttcaggaagt ctggcgcttt gatcccggct
     1321 gcccacacca tcagatcagc ctcaatatat tcgccatctt tagtgtgcag gccgccttca
     1381 tcagcactgg tgaccatggt ttgcgtcagc acgcgaacgc caagtttcgt tagctcgttg
     1441 tgggccgcag cagagatacg tggcggtaat gcaggcaaaa tacgttctcc cgcttctacc
     1501 agcgttacgt tcagggcttc gttggtcagg cctttgtaac cgtagctgtg cagttgcttg
     1561 accgcgttgt gcaattcagc ggagagttct acacccgtcg cgccgccgcc gacaatcgca
     1621 atgttcactt tgccatttgc gcccaggttg gcggagtatt tcaggaagag attcagcatc
     1681 tcctggtgaa agcgacgcgc ctggtgcggg ttatcgagga aaatgcagtt ctctttgaca
     1741 cctggcgtat tgaaatcgtt agaggtgcta cccagcgcca ttaccagggt gtcataggcg
     1801 attttacgtt ccggaaccag cagttcacct ttctcatcgc gcagttctgc aatagtgatt
     1861 gttttcgctt cacgatcaat atcaatgacg gaacccagct ggaactggaa accatgattg
     1921 cgcgcatggg ccagatagct caacgcatcg acgccttcat caagcgagcc agtcgccact
     1981 tcgtgcagca gcggtttcca caggtggctg tggttacgat cgaccagcgt aattttggct
     2041 tttttcttac gtcccagctt atgccccagc tgtgttgcca tttccagccc accagcaccg
     2101 ccgccgacaa tcacaatctt tttcaatggc gtagtcaacg tgaccccctt aaatttatta
     2161 accaattgtt aattaatggt tattaacata gctcttaatt aacaacaggt tacagcaatg
     2221 aaaatgttct tcgctattga gaataacatg aatggtgcat tggtcatacc aaaattgatg
     2281 ttaatcaagt tttgttgctg aaaagatagg cgactttacc gggtgagatc aaaaaataga
     2341 gtttattgtt tattatttga gcaaattcgg aagagtaata tagggtgaag cgtatatcga
     2401 gggcgtttgg tcgtcctgat aagatgcgca gcatcgcatc aggcattgat gctcgactgt
     2461 cggatgcggc gtggacgcct tatccgactt acagaattcg gagcgcatca ggtaccggta
     2521 aggttacctg atgcgacgag ggcatttacc cgagggtttt gaacgcttta atgcgctgta
     2581 aatgcgggga gatattcttg aatttgtgcg tctgctcttc gtcccagaca atctcgtaat
     2641 aatgatgcaa ctcttcagat gtccgctggc tgttaagtgc ttcatcatta cgcgacaaaa
     2701 taaccagaca acgatcgcga ttcttctcgc ggaagttggt cacacactta gtggcaatat
     2761 cggcatactc ttccgggcga tcaatcttgc cttccatgtt ctcataaggg aacaaattag
     2821 ggttgaagat cacctggcgg atatcgcaaa gaaaaccaat ccgttccgcc cagtatccgc
     2881 ccaggccaac gccgcaaatt agcggacgct cgtcaacgtt cagttgcaac attttgtcca
     2941 cttctttaag cagatgctgc atatcatgtt tcggatgccg cgtactgtag cttatcaagc
     3001 gtacatccgg gtcaataaac tgcaattgta agactttctc gtggttaccc ggactgttag
     3061 agtcaaaacc gtgtaaatag ataatcatcg cttcctcaca taagccaggg ttagtgacct
     3121 gctttctctt cctgccagcg ttcgtgtaac tgattcagac gggtgctgat cgctttccag
     3181 cgagccgagt ccatcagttc ctgtcgcgaa aatgaacctt tatgatacaa acgtgtaaca
     3241 cgttctgcct tgatcggtga cagattatct aacacgctga cggccccttt acgattattg
     3301 cagaccagga tcatatcgca acccgcatcc agtgatgcct gcccgcgttc ggcataactg
     3361 cccataatcg cggcaccttc catcgataaa tcgtcagaga aaatcacgcc gtcaaaaccc
     3421 agttcctgac gcaaaacggt tttcagccag tagggagaac cgctcgccgg acgcgaatca
     3481 acatcactgt agatcacatg cgcaggcata atggcgtcga gtttattttc gcggattaag
     3541 gaactgaaga ccgacatatc tttagcgcga atctccgctt gtggacgtgg atcgcacggt
     3601 gtttctttgt gtgagtctgc cgttactgca ccgtgtcctg ggaagtgttt cccggtcgtt
     3661 ttcattccgg cttcatgcat accatcaata aaccggctgg caattgccag ggctttttgt
     3721 ggatcggcat gataagaacg ctcgccaatc gccgcgctga tatgcccgac atccagcact
     3781 ggcgcaaagc tgatatcaat atccatagcg atcatttcgc tggccatcag ccaacccgcc
     3841 tcttgcgcca gtttgccgcc ctcttccatt cctgacagcg cagcgaatga ttgcgccgct
     3901 ggcaagcggg taaaaccttc acgaaaacgc tgcacgcgtc caccttcctg atctaccgcc
     3961 accaccagat gattgcgcga agctgcgcgg atctggcgca ccagttcacg taactgggca
     4021 ggatcatgat agttacgcgt aaagagaatc agccctccca ccagcggatg cgcaagtatt
     4081 tcacgctctt ccgcgtccag ttcgtaacct ttgacatcca acattactgg acccacactg
     4141 ctctccttat tgttttatta atagctgccg ccaggtgtca tcggccagcc tgataaattg
     4201 ttgatcgccg gtttgtcgcc agcggtactc aaaccaccct gctttgagca tcagcagcca
     4261 gggaaaccat cgcctgacct gacgccataa ttgcgccgga taaatcttcg cgcgagtggc
     4321 atagtcattg accaattgcc ggtgctgttc agtattttcc acccacaccg ccgccagttc
     4381 cagcgcgata tcaccatctc ctgcatactc ccagtcgatg agttttaacc ctgacgcgct
     4441 atgcactaaa tttccggcgt ggacatccat atgcaatgga cttaagcgta aaggccgtgg
     4501 ttcccgcgct ttgcgcagac gttttaacat tcgcagccaa cccactgtcc gccgcgccgg
     4561 atcgctttgc tgccagtaca gttccagtaa cggcaacagc gttattcgcc agccaaaacg
     4621 tggttgttga tgtagataat acagcaagcc tgccagttcg ttggtatccg gcaaatacgt
     4681 ttttaccgcg ccgggcagat agtcgactac catccagtca cggagatata aatgcggctt
     4741 cggtgcaatg catgcgggta gttgtgataa agcccgatac tggcgcaaga acgcggactg
     4801 cggcgcatca ggatcgtgcg gctgacgcac aacaaaacgc tggccctggt gttcaatgag
     4861 aaaactcccg ccactaagcc cactattaaa cgtcgtgacg ggatgaaact gcgggaaaaa
     4921 gcgcgacagc aattcgtcgc gcgtgatggg attattgctg cgaaacggca cctttacctg
     4981 accagataat ttcgcccgtc tgcaccagca tcagctgcat ttgtagggtc ggagcgttaa
     5041 cgttgccaga ggcgctggag tatagcacgt aatgagcgcc gacattgcgg gcaatgccta
     5101 tggctttact acgggtacct aaactgtcct gcggcgacaa acctaactgt tgcttagcca
     5161 tcgacagctg ctgggcggaa accagggtaa atttaccgtt attagccagc gcatttcgca
     5221 gcgtttcggt cgcttctgcg gcattcagcg aaccgttagt acggttgtta acgctatcaa
     5281 ccagcaggac gctacccgca gtcaccccgt cagccccaag catcttactg accatcggct
     5341 gcattgcgcc attccagtca taatggcgaa tatgcggcgc aggcggtgca gtttgatctt
     5401 cgtgctcaat tgggcctggc tgctgcggga tcgtcggcac cgagggcact gtggggacag
     5461 gctgttgtgg ctcggctggt tgttccggcg ctggtttcac ttcttctacc ggtgcaggtt
     5521 cacgttgccc cacacacccg gcgagaaaca tcgccagcgc ggtaatcaag gcgtagcgac
     5581 tcatttttgt catcaagatt caccccttac aaatatagat aaagtctgac tttgtgcgcc
     5641 cccagaaaat tggcgctgcc gtacagcgtt accgccgaat gtgcgggaat ggtaacgctg
     5701 cgtggccttt ccagaggatg catctccagc cctctggcgt cataccagta aaaacgataa
     5761 tgaacggtaa tgggttcttg cctttcgtta taaagcgttg aggatgctga aggttgaata
     5821 tcagacgtcg aaaggacggg cttttctgca ctgatgcccg cagccaataa agatgactcc
     5881 atcaccagcg attgctcatc attcaccgga atttccggat gtgaacgaca gcccaccagc
     5941 agtaacaacg ccagagacac cagcccaaag catccttttc tcatcgttac agacctttat
     6001 gcgccagcat tggtcccagc ggacggccac ccaacaagtg catatggatg tggtaaacct
     6061 cttgtccgcc atggcggttg gtattcatga tcagacgata gccatcttcg gcaatacctt
     6121 cttgctcagc aatttttgcc gctacggtga tcatgcgtcc cagcgcctgc tcatgctcag
     6181 ctgacacgtc gttcacagtc ggaatgagga tattcggaat gatcagaata tgcgttggcg
     6241 cttgcggcga aatatcgcga aacgccgtta ccagatcatc ctggtagacg atatcggagg
     6301 ggatctcacg acgaataatt ttgctgaata tagtttcttc tgccacgaca ttttcctttt
     6361 tcaaatagcc atgcgttgcg ccatgctacg ccgcgccaca ctgcgagtat agagtatggt
     6421 cgagttaaac ctgctctttc aaccttaact cacgatttct taagcaaaaa atcgttttgc
     6481 tgccgtatat atcgccatta ttcccatttc tgcctgcgaa tgcgctgaaa agacattctc
     6541 tgtttggttt acacgacaaa tatgaatacg tatatttctc atttgcattt acaagcaaaa
     6601 ttattcgcac actaaatata accgctcatc accctcctcc ggcatgagcc tgacgacaac
     6661 ataaaccaag agatttcaaa tgctttcaac acaatttaac agggataatc aatatcaagc
     6721 catcaccaaa ccctcactac ttgccggttg catagcattg gcactattac cttctgccgc
     6781 ttttgctgca ccagccactg aagaaacggt gattgttgag ggttcagcca cagctccaga
     6841 tgatggcgaa aatgattaca gcgtaacgtc tacctctgcg ggtaccaaaa tgcagatgac
     6901 tcaacgtgat attcctcagt cggtcactat tgttagccag cagcggatgg aagatcagca
     6961 gttacaaacg ctgggcgaag tgatggaaaa cacgctgggg atcagcaaaa gtcaggcgga
     7021 ttccgatcgt gctctttatt attcccgcgg attccagatc gataactata tggttgatgg
     7081 tatccccacc tattttgaat cgcgctggaa tctgggcgac gcactttctg atatggcact
     7141 gtttgaacgc gtagaagtag tgcgtggcgc gacaggactc atgaccggga cgggtaatcc
     7201 atctgcggca attaatatgg ttcgaaaaca cgcgaccagt cgtgaattta aaggcgatgt
     7261 ctcggcggaa tacggtagct ggaacaaaga acggtatgtg gcggatttac aaagcccact
     7321 caccgaagac ggtaaaatcc gcgcgcgaat tgtcggcggc taccagaata acgactcatg
     7381 gctggaccgc tacaacagtg aaaagacctt cttctcgggc attgtcgatg ctgatttagg
     7441 cgatcttacg acgctttcag ccggttacga atatcagcgc attgatgtta atagccctac
     7501 ctggggcggt ttaccgcgct ggaatactga tggcagcagc aacagttacg atcgtgcacg
     7561 cagtaccgca cctgactggg cgtacaacga taaagagatc aacaaggtct ttatgaccct
     7621 gaagcagcgg tttgctgata cctggcaagc gacactgaat gccacccact ctgaagtcga
     7681 atttgacagc aaaatgatgt atgtcgatgc ctatgtaaac aaagcggatg gtatgctggt
     7741 tgggccatac agtaattatg gacctggctt tgattatgtc ggcggcaccg gttggaacag
     7801 tggcaaacgt aaagttgatg cgctggattt gttcgctgac ggtatttatg aattgtttgg
     7861 tcgtcagcac aatctaatgt ttggtggcag ttacagcaaa caaaacaatc gttacttcag
     7921 ttcatgggcc aacatcttcc cggatgaaat tggcagtttc tacaacttta atggcaattt
     7981 cccacaaacc gactggtcac cacagagcct ggcacaggac gataccacac atatgaaatc
     8041 gttatatgct gccactcgag tcacccttgc cgatccgctg catctgatcc tcggcgcacg
     8101 ttataccaac tggcgggttg atacgctgac ttacagcatg gagaaaaacc acaccacgcc
     8161 ttacgctggt ctggtgtttg acatcaatga caactggtcg acctacgcca gctatacctc
     8221 tattttccag ccgcaaaatg atcgtgacag ttcaggcaaa tatctggctc caatcaccgg
     8281 taacaactac gagctgggtc tgaaatcgga ctggatgaat agccgtctga ccaccacgtt
     8341 agccatcttc cgtattgagc aggataatgt cgctcagtcc accggtacac ctatccccgg
     8401 cagcaacggc gaaaccgcct ataaagcggt ggatgggaca gtcagtaaag gggtggaatt
     8461 tgaactcaac ggcgcaatta ccgacaactg gcagctgaca tttggcgcaa cgcgctatat
     8521 tgcagaggat aacgaaggaa acgccgttaa tcctaatctg ccacgcacca cggttaaaat
     8581 gttcaccagc tatcggttgc ctgtcatgcc agagttgaca gtcggcggtg gtgttaactg
     8641 gcaaaatcgc gtgtataccg acaccgtgac accgtatggc accttccgcg ccgagcaagg
     8701 tagctacgcg ctggtggatc tcttcacccg ctaccaggtg acgaaaaact tctcgttaca
     8761 ggggaacgtc aataacctgt tcgacaaaac ctacgatacc aacgtggaag gttctatcgt
     8821 ctacggcaca ccgcgtaatt tcagcattac cagcacgtat caattctgaa taacacctgt
     8881 aaaaaaggca gccatctggc tgccttagtc tccccaacgt cttacggatt agtggttacg
     8941 gatgtactca tccatctcgg ttttcaggtt atcggattta gtaccgaaaa tcgcctgaac
     9001 accagaacca gcaaccacta cgcccgctgc gcccagtttc ttcaggccgg cctgatccac
     9061 tttagacaca tcagcaacgc tgacgcgcag acgggtaata catgcgtcga ggttagtaat
     9121 gttttcttta ccaccaaatg cagcaaccag agccggtgcc atttcgctgg tacctgtcgc
     9181 ttttgcatct tcagtcgcgt cttcacgacc cggcgttttc agatccagtg ctttaatcag
     9241 cacgcggaag atggtgtagt aaacaatcgc ataaccgata ccgacgatcg ggaacagcca
     9301 cagtttgctg ctgttaccag acagaacgat gaagtcgatc agaccgtgcg agaacgacgt
     9361 accgtcacgc atccccagaa gaatacagat tgggaatgcc aggcctgcca gaatcgcgtg
     9421 gatgatgtac aggatcggcg caacgaacat gaaggagaac tcaatcggct cggtgatacc
     9481 ggtcaggaac gaggtcagcg ccgcggaaat cataataccg cccactttcg cgcggttttc
     9541 tggtttagca gagtgccaga tagcaattgc ggcagctggc agaccgtaca ttttgaacag
     9601 gaagccacca gacagtttac ccgcagtcgg gtcacccgcc atataacgcg gaatgtcgcc
     9661 gtggaaaacc tgacctgctg cgttggtgta ttcaccaatc tgcatctgga aaggtacgtt
     9721 ccagatgtgg tgcagaccaa acggtaccag gcaacgttcg atgaaaccgt aaatgccaaa
     9781 cgcaactacc gggttctggt aagcagccca ctgagagaag gtctggattg cagaaccaat
     9841 cggcggccaa atgaaggaca gcacaacgcc agtaaagatg gcagccaggc cagaaatgat
     9901 cggcacaaag cgtttaccgg caaagaagcc aagatactca ggcagcttaa tacggtagaa
     9961 acggttaaac atgtacgctg cgatcgcacc ggagataatc cctccgagta cgccagtatc
    10021 cgccaggtgt ttagaggcga tttcttcagc aggtaaatgc agtaccagtg gcgcaaccac
    10081 ggccatggtt ttaaccatga tgccataggc aacaactgcg gccagcgcgg atacgccatc
    10141 gttattggta aagccgaggg cgacaccgat cgcaaaaatc agtggcatgt ttgcaaagac
    10201 ggaaccgcct gcttctgcca taacatgcga tacaacggcg ggcagccagc tgaaattcgc
    10261 ggaaccgacg cccagcagaa tacctgcgat aggcagtacg gataccggca gcatcagcga
    10321 tttaccgacc ttttgcaggt tagcaaatgc attcttaaac ataattgaga gtgctcctga
    10381 gtatgggtgc tttttttacg ttctcacgcg tggcaagggg ggagagcctc gccgtgtaca
    10441 gggcatctaa gcgcccttta tttattacac agagtaaaat aattcagtgc caatttgttt
    10501 gacggctatc acgtttcaac ttcagatcat tggagaagtt gcacattttg acaaaacata
    10561 tgtcacaata tttcaaacgc ggttattcac cgcccaattt tcggcggtga actttactcg
    10621 ttttagccat taattacgag ctttaaaaaa actcattcaa cggatggatt gaaggcggga
    10681 agcgtcgatg tgaaacagac gggcgaagtt atcggtggtt acctgcgcca gttcttcaac
    10741 ggcaacacct ttcaacacag ccatgtattc tgcaacgtca cgaaccatcg caggttgatt
    10801 ctcttttcct cgatgcggta ccggcgcaag gtaaggtgag tcagtttcca ccagtaatcg
    10861 atccaggggg acataacgcg cagcatcgcg cagttgctcc gcattacgga aggtcacaat
    10921 gccggaaaag gagatgtaaa atccgagatc cagtaattta cccgccgttt ctctgtcctc
    10981 tgtaaaacag tgtagtacgc caccgcaatc cgtcactttt tcttcgcgca gaattgccag
    11041 cgtatcggcg cgggcgtcac gggtatggac gataaccggc ttgttcagtt cacgaccaat
    11101 ctggatatga tggatgaagg actcttgctg acgtacttta gtttccggcg tgtaataata
    11161 atccagccca gtttcaccca gcgcaaccac accctcttct gccgccagac ggcgtaaatc
    11221 ttctacatcg tagggatcat tctggttgag tggatgcacg ccacaagaaa atacgacgtt
    11281 gtcacgttcg cctaccagat cccgcatgtg taaataaccc ggtaacgttg tggcgactgc
    11341 cagacaaaat ttcacatcgc gtgcggcggc tttcgccaga acgtcatcca cgtccttatg
    11401 caaagattca taatccagac catcgagatg gcagtgtgag tcgactaaaa acatgatgtc
    11461 tctcttaaag atgaggaacc ggtagcacaa cgcccggttg caggtaatgc tcaatacgca
    11521 gtaaaagatc ggtgatgaga agctcgcggt tgatgcctgt aacagacatt aactgttcac
    11581 gaatgtggca aacatccccc agtatagcct gcaggcgcga gggagaaaga tggtttgcca
    11641 gttcggcgac caggcccggc acatcaacat tggtcacctg cgcagcacca tgatggcgtt
    11701 ttagcgcatc catcagcaac gttgccagcc agtgtaaacg cgccggagct tgttcatgat
    11761 taagggccgc tagcagcgaa taccagtcgc ccgatggcac gctatatgcc aacgcctgac
    11821 acaatgtttc acgagcctgc cagttatctc cctgaaacaa cgccagtgcc gcgccaggcg
    11881 aaccggcgct taagcgcaat gcggcaagta atgcatcctg tgacattgtc acttcgcgtg
    11941 aaagccaggt cacggcgtac tgttccggcg gcggcgcaag gtaatgtaac cgacaacgac
    12001 tacgtaatgt tgccagtaaa cgttcaggct cgcgggtagc caggaaaaac caggtttctg
    12061 ctggtggctc ttcaagcgtt ttcagcaatg cgttagccgc ggcgtcggtt agtaaggcag
    12121 catcggttac ccagacaact ttcgcaccac ctaggcgtgc gtgctcattc agcttttcgg
    12181 tgacctcacg taccgcatca atgcccagca tatttttccc tttttcggga gccagggtgt
    12241 agtaatcggg atgcgtgcca gcctgcatca actgacatcc acgacagtga ccgcaacttt
    12301 tgtggccctg cggttgttgg cagagtaaat aacggctcag ggcgtagatt aaagcatcat
    12361 cgcccatgcc cggtaacgcc tgaatgagta gcgcatggtg acctcttccg gcctgatagc
    12421 tggctaccag tttttcgaaa tcaggtcgta accatggata ccatctcatg cgtccaactc
    12481 cttaacccag tgggtcacgg tagtgcggat tgcatccatc acggcctcca gcggctgggt
    12541 ggcatcaatg gtatgaatac ttttatcttg tgcagccagt tccagatagc gggcgcgggt
    12601 gcgattaaag aaatcgaaag actcttgctc aatacgatcc agctcgccgc gcgcacgcgc
    12661 gcgttttaag ccaacttccg gggtaacatc gagatagagc gttaagtcgg ggcgaaaatc
    12721 cccgagaaca gcatcacgca gtgttgccag catatgttgg tcaataccac gtcctccgcc
    12781 ctgatacgcc tgagtggaga gatcgtggcg atcgccaatc acccaggtgc cgttagccag
    12841 agctggtttg atgaccgttt ctaccagttg aacgcgcgcg gcataaaaca tcagaacttc
    12901 ggctttatcg gtaatgactt catcgcctac cgatttgata tccagcacca ggcttcttaa
    12961 cttttcggca agttgcgtac cgccaggttc ccgagtgaaa accatgtcgc ggatacccag
    13021 ttgctcaagc gtctcaacca ccacattacg cgcggtagtt ttgcctgcgc cttccagccc
    13081 ctcaatgacg atatacttac tgcgcatttt tttccttaag cactttcaga taatcctgca
    13141 cagacttgtt atggctggca agattggtat taaacgtgtg accaccttta ccatcggcca
    13201 caaaatagag atacggcgtt tttgccggat gcgcagcagc cttcagcgaa tccgcccc
//
LOCUS       DAMZET010000116        13169 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.116, whole genome shotgun sequence.
ACCESSION   DAMZET010000116 DAMZET010000000
VERSION     DAMZET010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.116"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..523)
                     /locus_tag="POB34_003346"
     CDS             complement(<1..523)
                     /locus_tag="POB34_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HDI5920233.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEAL"
     gene            complement(535..1896)
                     /gene="trpCF"
                     /locus_tag="POB34_003347"
     CDS             complement(535..1896)
                     /gene="trpCF"
                     /locus_tag="POB34_003347"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HDI5920234.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HSNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(1897..3492)
                     /gene="trpD"
                     /locus_tag="POB34_003348"
     CDS             complement(1897..3492)
                     /gene="trpD"
                     /locus_tag="POB34_003348"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HDI5920235.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     YQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(3492..5054)
                     /gene="trpE"
                     /locus_tag="POB34_003349"
     CDS             complement(3492..5054)
                     /gene="trpE"
                     /locus_tag="POB34_003349"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDI5920236.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(5075..5168)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(5145..5189)
                     /gene="trpL"
                     /locus_tag="POB34_003350"
     CDS             complement(5145..5189)
                     /gene="trpL"
                     /locus_tag="POB34_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HDI5920237.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            5327..6208
                     /gene="yciV"
                     /locus_tag="POB34_003351"
     CDS             5327..6208
                     /gene="yciV"
                     /locus_tag="POB34_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HDI5920238.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            6205..6825
                     /locus_tag="POB34_003352"
     CDS             6205..6825
                     /locus_tag="POB34_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDI5920239.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            6926..7798
                     /gene="rluB"
                     /locus_tag="POB34_003353"
     CDS             6926..7798
                     /gene="rluB"
                     /locus_tag="POB34_003353"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HDI5920240.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(7838..8428)
                     /gene="cobO"
                     /locus_tag="POB34_003354"
     CDS             complement(7838..8428)
                     /gene="cobO"
                     /locus_tag="POB34_003354"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HDI5920241.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(8425..9183)
                     /locus_tag="POB34_003355"
     CDS             complement(8425..9183)
                     /locus_tag="POB34_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HDI5920242.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRINCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            9403..10452
                     /gene="sohB"
                     /locus_tag="POB34_003356"
     CDS             9403..10452
                     /gene="sohB"
                     /locus_tag="POB34_003356"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HDI5920243.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(10488..10739)
                     /locus_tag="POB34_003357"
     CDS             complement(10488..10739)
                     /locus_tag="POB34_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HDI5920244.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            11119..>13169
                     /gene="topA"
                     /locus_tag="POB34_003358"
     CDS             11119..>13169
                     /gene="topA"
                     /locus_tag="POB34_003358"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDI5920245.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CG"
BASE COUNT         3175 a         3353 c         3545 g         3096 t
ORIGIN      
        1 gcagcgcctc gttacaggca tctttcagcg tcgcggaacc gctatgcacc gggatcactt
       61 ccgcacccat taagcgcata cgaaaaacgt taggcgactg gcgttctacg tctttggcac
      121 ccatataaat acggcatttc aggccgagca gggcgctggc aagggccgac gccacgccat
      181 gctgaccggc accggtttcg gcgatgattt cggttttacc catccgcttc gccagtaaag
      241 cctgaccgag cacctggtta gttttatgcg cgccgccgtg cagcaaatct tcacgcttga
      301 gatacagagt ggtgttcgtc ccggctgtaa tgttctggca tttggtcagc gcggttggac
      361 gcccggcata gtttttcagc aggtcgttga actgagcctg aaattcagga tctttttgcg
      421 cactgacaaa agcttcttcc agctggcgca gagcaggcat caggatttgt ggcacgtaca
      481 tgccgccaaa ctcaccaaaa taggggttaa gtaatgttgt cattgttcct ttccttaata
      541 tgcgcgcagc gtctggaaaa ccgaggccaa aagacgtgcg tctttgatgc ccggttgcga
      601 ctctacagca gaattaaaat caagtccggc gcagccggtt tgtgccgctt ccacgcagtt
      661 atctgcgcct aagccccccg ccagcagaac gttgccaagc gattgaccat ttaatagtga
      721 ccagtcgaaa cgttgcccgc ttccaccctg gccgttgtct aaaacatatt tatcaacgtg
      781 ctgaagctcg cgggcgggca gggtttcacc gacgcttaat gctttccaga tggcaacgtg
      841 tgctggcaga gcttcacgca gcgtatcgat atacagctga tcttcattac tatgcagttg
      901 cactgccgcc agcgataaca ccttagcttt gtccaccaca tcggcaatat cgtgattgcg
      961 gaacacgcca acatactgca acggtgccgc agccatcact tcctgcgcct gttcaacgtt
     1021 gacgcaacgt ggtgatgtcg caacaaaaat caacccaccg taaatcgcgc ccgcgtcata
     1081 agctgcttta gcatcttgcc cacgcgtcag gccacatact ttattctcac ccagcaacac
     1141 ccggcgcacg gcggcgtgca aatcgtcatg ggccatcaac gccgaaccaa tcagaaaacc
     1201 gttagcgaag tggcttaact cgcgcacctg agcgtaagta ttgatgccgg attcgctgat
     1261 taccgttacg ttgtgcccca gtttcggcgc aagctcgcgg gtacggttga gatcaatcga
     1321 caaatcacgc agatcgcggt tgttgatgcc aacgaccttt gcccccaatg caatggcacg
     1381 ctccagttcc tcttcattac tgacttcggt cagcacaccc atctccagac tgtgggcgac
     1441 ggcggcaagc tggcgatatt gttcgtcatc cagtactgaa agcattaata agcaggcatc
     1501 agcctggtaa tagcgcgcca gatagatctg gtaaggatcg ataatgaagt ctttacataa
     1561 aatcggctgc ggggcgattt ggctgacgat ggggaggaaa tcaaagctcc cctgaaaata
     1621 tttctcatca gtcagcaccg aaattgccga agcgtaatgt ttataaatgg cggcaatgcg
     1681 tgctggatcg aaatcatcac ggatcacgcc ttttgacggc gacgctttct tgcactccag
     1741 aataaacgcc gtgcgcgcac cctgtagcgc atcataaaaa tgtcgcgtgc tcggctgaac
     1801 ctcattctga aaactggcca gcggttgctg ctgtttgcgg gtttctaccc aaatcgcctt
     1861 gtctgcgacg attttcgcta aaacggtttg catcatttac cctcgtgccg ccagcgcggt
     1921 aactctgtcg taagcggaac cactgcgcag tacctcaaga acggtttgcg cattggcttg
     1981 cagatcttca tggccatgca ggcgcattaa catggcgacg ttcgcagcga cggctgcttc
     2041 atgggcggcg tcgcctttac cttgtaacaa acgtgttaaa atgtcacggt tttcttccgg
     2101 tgttccgcct gccagttgct cctggtggta gggtgtcagg ccaaaatctt cagcggtcaa
     2161 ttgatagctc ttaatttcgc cgtcatgcag ctcggcaacg attgtcggcg cgtgtaatga
     2221 aacttcatcc atcccgccgc tgtgcaccac cgccgcgcgt tgatacccca gcacgcgcaa
     2281 ggtttcggca atcggcagca ccagttccgg actataaaca ccaattaacg ccagcggcgg
     2341 atgcgccggg ttaatcaatg gccccagcac attgaacagg gtgcgggttt ttagttgctg
     2401 gcgaaccggc attgcatggc gaaatccggt gtgatatttc ggtgcaaaga ggaaacatac
     2461 gcctaactca tccagcgcct ggcgcgattt atcggcgttc atatcaagat taataccgaa
     2521 cgccgccagc agatcggacg aaccagattt actggagacg ctacggttgc cgtgtttcgc
     2581 cactttcagc ccacaggccg cggcgacaaa cgcactggcg gtagaaatat tgatactgtt
     2641 gctgccgtca ccgccagtac cgacgatatc agcaaacaga taatccgggc gcgggaacgg
     2701 cgctgcgttt tccagtagcg cggttgctgc cccggcgatc tcgttcgggt gctcaccgcg
     2761 aattttcatg ctcaccagcg ccgccgccag ttgttccggc ttcagctcgc cacgcaccac
     2821 cgctgaaaac agctggtagc tttcttgttg gctaagcgtc tgcgcctgat acagtttttc
     2881 cagaatcggt tgcagcgtgt tggctggctc tagtttctgc tgcgcccagg ccagcgtttg
     2941 ttccagcagg cgagcgccct gggtggtgag aatggattcc ggatggaact ggaatccaca
     3001 aacgcgatcc gcatcgtgac gtactgccat caccatgcca ttaaaatggg cgttgatggt
     3061 taaaccggcc ggaatgttac tgccaaccag cgagtgataa cgcgccaccg gcagcgggtt
     3121 tgttaatccg gcaaacatcg cctgaccgtc atgttcaatg ctcgacgctt taccgtgaag
     3181 aatttcgccc gcctgaccga catagccccc gtaagcttcg acaattgcct gatgtccgag
     3241 gcaaatgcca ataatgggca gcttgccacg caagcgggtg aggagttccg gcatacaacc
     3301 ggcttcgctc ggcacaccgg ggccaggaga aagcatcagt accggattgc tcatggtcgc
     3361 caggcgttca attaaggtct gcgccggaat atggttgcgg taaatcacca cgttatgccc
     3421 attgctgcgc aactgatctg ccaggttgta cgtaaaagag tcgatattat cgagcagcag
     3481 tatgtcagcc atcagaaagt ctcctgtgca tgatgcgcgg tggcaatagc gcgcagtaca
     3541 gcgcgggctt tattacgggt ttcgtcggct tccgactgcg gaatagaatc aaggactacg
     3601 ccagcaccgg cttgcacggt ggcgataccg ttttccacca gcgccgagcg gatcacaatg
     3661 caggtgtcga gatcgccgtg cgcggtgaaa taacctaccg cgccgccgta gctgccgcgg
     3721 cgacgacctt ccgcctcggc aattaactgc atggcgcgca ctttcggcgc accgcttaac
     3781 gtccccatat tcatacaggc gcggtaagcg tgtagggcgt cgagatcgtg gcgcagctca
     3841 ccgaccacgc gggagaccag gtgcatcacg taagagtaac ggtcaacttt ggtgaggtcg
     3901 gcgacgtagc ggctgccggg ggtgcaaatg cgtgccagat cattacgggc aagatccacc
     3961 agcatcagat gttcagaaag ctctttatga tcggtacgca tttccagttc gatgcggctg
     4021 tcgaggtctc tgtccagcga gccatcggca cgacgaccgc gtggacgtgt tccggcaatc
     4081 gggtagatct caatctggcg gctggtggcg tcatacttga gcgaactttc cggcgacgcg
     4141 ccaaacaggg tgaaatcatt atcctgcata aaaaacatgt acgggctggg attactcttt
     4201 ttcagcacgt aataggccgc cagcggtgac gggcagggca gagagaaacg gcgagatggc
     4261 accacctgga aaatttctcc agcgcgaatc gctttttgca acaaacgcac tacgccaccg
     4321 aactcttcat cgctctgatt acattcacaa cgcatatgcg gcacggaaac caccggcagc
     4381 ggcggcgcgg cttcggtcag ttgctgacgt agttcgttca ggcgagcagt gagacgttgt
     4441 ttttcttctt cattcggagc aaacaggctg gcctgaatac gggtgctttt tttctgatgg
     4501 tcaatcacca tcagagtttc agcgagataa aaacagaaat cagggcagtt attttccgct
     4561 gacagttgcg gtaaatcttc aaatcccgcc acaaggtcat aagagaacag gccgccgaag
     4621 aacattgctt ctcgttcttc cttcggtaca ttcaacagat tctgcaataa acggaaagcg
     4681 tcaaaaaccg aaagggagca taagcgagcg tcttcatcca gcagtggact gacagggggg
     4741 aagcgcagca cacggcagtt tggtaattgt tcattttcca cacccgcagg cagggcgtta
     4801 tccagtagtg tcagcagggc ttcgccgttg ccggaaagtg cctggattgt gacagtgtca
     4861 cctaaagctg taatgcgcag cgcactgtct accagcagca ggctttttaa atcatctttg
     4921 ctgtcgatat ctgcggattc cagcagcagc gttgccggac gatccccaca caactggtga
     4981 aaaagcgcgg tgggattgtc gcgataagcg ccttcgcagg ttagcagttc gagagtcggt
     5041 ttttgtgttt gcattgttat tctctaattt tgttcaaaaa aagcccgctc attaggcggg
     5101 ctgggtatct gattgcttta cgcatggtga atacactgcc cgtttcagga agtgcgccac
     5161 caacctttca gtacgaaaat tgctttcatt gtcgataccc tttttacgtg aacttgcgta
     5221 ctagttaact agttcgatga ttaattgtca acagctcatt tcagaatatt tgccagaacc
     5281 gttatgatgt cggcgcaaaa aacattatcc agaacgggag tgcgccttga gcgacacgaa
     5341 ttatgcagtg atttacgacc tgcacagcca taccacagct tccgatggct gcctgacgcc
     5401 agaagcattg gtgcaccgtg cagtcgagat gcgcgtcggc accctggcga tcaccgacca
     5461 tgacaccaca gctgcaatcg cgccagcaag agaggaaatt tcacgttcgg gactggcgtt
     5521 gaatcttatt cccggcgtgg aaatttccac ggtctgggaa aatcatgaaa ttcatattgt
     5581 cgggctgaat attgatatta ctcatccgct aatgtgtgag ttcctcgcgc agcagacaga
     5641 acggcgcaat cagcgggcgc agctgattgc cgaaagactt gaaaaagcgc aaatacctgg
     5701 ggcgctggaa ggcgcacaac gactggcgca gggcggcgca gtgacgcgcg gtcattttgc
     5761 gcgtttcctt gttgagtgcg gcaaagccag ttcaatggcg gatgtcttta aaaagtatct
     5821 ggcgcgcggg aaaaccggat acgttccgcc acagtggtgt acaatagaac aagctattga
     5881 tgtcattcat cattctggcg gtaaggcggt gctagctcat cctgggcggt acaatcttag
     5941 tgctaaatgg ctgaaaagat tggttgcgca ttttgccgaa caccacggtg acgcgatgga
     6001 agtcgcgcag tgccagcaat cgcccaatga acgtacccag ctggcggccc ttgcgcgtca
     6061 gcatcattta tgggcatcac aaggatctga ttttcatcag ccatgcccgt ggatcgaact
     6121 gggtcgtaaa ctctggctgc ccgcaggcgt tgaaggcgtc tggcagctat gggaacagcc
     6181 gcagaacacc acagagaggg aattatgagc cagttttttt atattcatcc tgataaccca
     6241 cagcaacgtc tgatcaacca ggcggtggag atcgtgcgta aaggcggggt gattgtttat
     6301 ccaactgatt ccggctatgc gctcggctgt aaaattgaag ataaaaacgc gatggagcgt
     6361 atttgtcgta ttcgccagct gccggacggt cacaacttta ccctgatgtg tcgcgatctt
     6421 tctgaactct cgacctattc atttgttgat aacgttgcgt ttcgtttaat gaaaaacaac
     6481 acgccgggca actatacctt catcttgaag gggacgaagg aagtgccacg ccgtctgttg
     6541 caggaaaaac gcaaaaccat cggtatgcgt gtgccttcta acccgattgc gcaggcgttg
     6601 cttgaagcac tgggcgaacc gatgctttcc acttcgctaa tgctgccagg cagcgaattt
     6661 accgaatccg atcccgaaga aattaaagac cgtctggaaa aacaggtgga tttgattatc
     6721 cacggcggct atctcggaca gaaaccgaca acggttattg atcttaccga tgatacgccg
     6781 gtcgtggtgc gtgaaggcgt aggtgatgtg aagcctttct tataagtgcg taactctgta
     6841 tactacgcgg ccttaaaatg gcgctatacg gcgccattga ttctcctgtt cgacgcctgt
     6901 gaaggcgaca ccaaaaggaa gctctatgag cgaaaaatta cagaaagtgc tggcccgcgc
     6961 tggccacggc tcccgccgtg aaatcgaatc tattattgaa gccggtcgtg tgagtgttga
     7021 tggcaaaatt gctaaactcg gcgatcgcgt tgaagttacc cctgggctga aaattcgtat
     7081 cgatggtcat ctgatttcgg tacgtgagtc cgctgaacaa atttgtcgcg ttctggccta
     7141 ttacaaaccg gaaggtgagt tgtgtacccg taacgatccg gaagggcgtc cgacggtgtt
     7201 tgaccgtctg ccaaagctgc gtggcgcacg ctggattgcc gtaggtcgtc tggacgttaa
     7261 cacctgtggt ctgctgctgt tcaccaccga tggtgaactg gcaaaccgtt taatgcatcc
     7321 aagccgtgaa gttgaacgtg aatacgccgt gcgtgtattt ggtcaggttg acgacgcgaa
     7381 actgcgtgat ttgagtcgtg gcgtgcaact ggaagatggt ccggcagctt ttaaaaccat
     7441 caagttcagc ggcggcgaag ggatcaacca gtggtacaac gtgactctga ccgaaggccg
     7501 taaccgtgaa gttcgtcgtc tgtgggaagc ggttggtgtg caggtgagcc gcctgatccg
     7561 tgttcgttac ggtgatatcc cactgccaaa aggtctgcca cgcggtggct ggacggagct
     7621 ggacctcgcc cagactaact atctgcgcga actggtggag cttccgccag aaaccagctc
     7681 taaagtcgct gtagaaaaag accgtcgtcg catgaaggct aatcagattc gtcgtgcggt
     7741 gaaacgtcac agtcaggtga gcggcggtcg tcgttctggc gggcgtaata acggttaatc
     7801 aaagtatgcc ggacgtcata tccggcattt ttacagatta ataatcaatc cctatttgcg
     7861 ccttaacacc ggcatcaaac gcatgtttga tggggcgcag ctcacttacc gtatccgcca
     7921 gttcaagaat atcccgatga cagcctcgac ccgtgataat caccgtctgt tgatgtggac
     7981 gttcatttaa cgcctgcacc acttcttcca gtggcaaata gtcatacgcc accatatacg
     8041 tcagttcatc aagcaaaacc atatccagtg aggaatcagc aagcatccgc tttgcatgtt
     8101 gccagacttc gcggcaggcg gcggtatcag actcgcggtt ttgtgtatcc caggtaaagc
     8161 ccgttgccat cacctgaaac tcaacgccat gtggctccag cagattgcgt tcgccattag
     8221 gccaggtgcc tttaataaac tgcacgacgc ctactttttt tccgtgacca actgcgcgtg
     8281 ttgccgtacc aaatgccgcg gtggttttgc cttttccatt gccggtaaag acgatgataa
     8341 taccgcgttc atcctgggcc tgggccacac gagcatctac tttttctttc actcgctgct
     8401 gacgctgttg gtagcgttca tcactcattg ggaaattcct ggtttacggc ccggttgggc
     8461 gtcaaaggtc atgccggttt tacggcggct gtcatcgccc atcagccaga ggtagagcgg
     8521 catgatatca gcgggtgttt taagtttctg tggatcttcg gtcgggaagg cgctggcacg
     8581 cattgcggtg cgcgtaccgc ctgggttaat gcagttgatg cgcaggcgct gctgatattc
     8641 atcggccagt acctgcatca tcccttcggt ggcaaatttc gacgctgcat atgcgcccca
     8701 gttggctcgt ccctgacgtc caacgcttga tgaagtaaag accagtgaac cggcgtccga
     8761 tttgagtaat aaaggaagca gtgcctgggt gagcataaag gtggcattaa cgttgacctg
     8821 tatgacgtcc tgccagacct gtggattttg ttcgctcatt gggcaaacat cgccgagcaa
     8881 tccggcatta tgcaaaacac catccagacg cggataatta acggtaatgc gctgtgccag
     8941 ttgttggcaa tcttcggacg tgcaggtcag caaatcgaga ataaaccact gtggctgacg
     9001 tccagtttct tcgtttatgt ggctggctac ctgacgtaat ttttcttcat tacggcccaa
     9061 cagaatcact gtcgcaccat agcgtgcata cgtcatcgcg gcttcacggc caatcccatc
     9121 gctggctccc gtcaccagga taatgcgatc attgagtaaa tcttgttttg gctggtaatg
     9181 catggctact cctcaacgac gttgtctgtc gtgctgccag tgtactttat gactttgggg
     9241 ctttatgcct gaaacagaag ggcatttcaa tcagcctggg gcgataacac cgcaaaatgg
     9301 atactttgtc atactttcgc tgcaataaca tctctgcgag acggcttaac atgcctgttg
     9361 taaactgtga gccaaagcgt tgtttaacca aggtggggac tcgtggaatt gttgtctgaa
     9421 tatggtttgt ttttggcgaa aatcgttacc gttgtgctag cgattgcggc gattgccgcc
     9481 attattgtca atgttgctca acgtaataaa cgccagcgtg gcgagttacg ggtcaacaat
     9541 ctcagcgaac agtataagga gatgaaagaa gaactggccg cggcgctgat ggactcacat
     9601 cagcaaaaac agtggcacaa agcgcagaag aaaaagcaca agcaagaagc gaaagcagca
     9661 aaagcgaaag ccaaactggg cgaggtggca actgacagta aacctcgcgt ctgggtgctg
     9721 gattttaaag gcagcatgga cgcccatgaa gtgaactcgc tacgtgaaga gataacggcg
     9781 gtactcgcag cattcaaacc gcaggatcag gttgtgctcc gtctggaaag ccctggtggc
     9841 atggtgcatg gttacgggtt ggcggcttcg cagctgcagc gtctgcgtga taaaaacatt
     9901 cctttaactg ttacggtaga caaagtcgct gccagcggcg gttacatgat ggcctgtgtg
     9961 gcggacaaaa ttgtttccgc accgtttgct attgtgggtt ccattggggt ggtggcgcaa
    10021 atgcccaact ttaaccgctt cctgaaaagc aaagatattg atatcgaact gcacactgcc
    10081 gggcagtata agcgtactct gacgttgctg ggtgaaaata ccgaagaagg gcgggagaaa
    10141 ttccgcgaag agttgaacga aacgcatcag ttgtttaaag attttgtgaa gcgtatgcgt
    10201 ccgtctctgg atattgaaca ggtggcaacg ggtgaacact ggtacggaca acaggcggta
    10261 gagaaaggcc tggttgatga aatcaacacc agtgatgaag ttattcttag cctgatggaa
    10321 ggccgtgaag tggttaatgt acgctatatg cagcgtaaac gactcattga ccgattcacc
    10381 ggcagcgcgg cagagagcgc cgatcgattg ttgctacgct ggtggcagcg gggtcaaaag
    10441 ccattgatgt aaaagacaaa cgcgaggcta agacctcgcg ttttgcttta atcaaccaga
    10501 tgatattttt ctgaaagcac atgggccagg tgtttgaaca tattaaacac cgcggtgctt
    10561 ttggctgttg gcaatccttg ttcatctaaa aagtagtcgc cggtaaatac cagcacgcca
    10621 ttacgctgcg tgacgccggt ggcttcaatc cctgcgagcg tatcctcatg ctcacgaatg
    10681 attttgttgg cctctttcaa cagcgtttcg cggtcgatgg gttgtgtctc tttgttcatt
    10741 atttactcct taaacaagga cattagtcta cgccaggcat ggcttgcaga caaatatacc
    10801 acgctggtgg caagagcgcc ttactggtaa ctttggattt tgcatgctaa taaagttgcg
    10861 tatcggattt tatcaggtac agtgtgacgc tttcgtcaat ctggcaatag atttgcttga
    10921 cattcgacca aaattccgtc gtgctatagc gcctgtaggc caagacctgt taactcagtc
    10981 acctgaattt tcgtgaacag agtcacgaca aggggttgat atccgcagag aacgagtcca
    11041 tatcggtaac tcgttgccag tggaaggttt atcaacgtgc gacgcattcc tggaagaatc
    11101 aaattaggta aggtgaatat gggtaaagct cttgtcatcg ttgagtcccc ggcaaaagcc
    11161 aaaacgatca acaagtatct gggtagtgac tacgtggtga aatccagcgt cggtcacatc
    11221 cgcgatttgc cgaccagtgg ctcagctgcc aaaaagagtg ccgactctac ctccaccaag
    11281 acggctaaaa agcctaaaaa ggatgaacgt ggcgctctcg tcaaccgtat gggggttgac
    11341 ccgtggcaca attgggaggc gcactatgaa gtgttgcctg gtaaagagaa ggtcgtctct
    11401 gaactgaaac aactggctga aaaagccgac cacatctatc tcgcaaccga ccttgaccgc
    11461 gaaggggaag ccattgcatg gcacctgcgg gaagtgattg gtggtgatga tgcgcgctat
    11521 agccgagtgg tgtttaacga aattactaaa aacgcgatcc gccaggcatt taacaaaccg
    11581 ggtgagctga atattgatcg tgttaatgcc cagcaggcgc gtcgctttat ggaccgcgtg
    11641 gttgggtata tggtttcgcc gctgctatgg aaaaagatcg ctcgtggtct gtctgccggt
    11701 cgtgtgcagt cggtggcggt tcgcctggtg gtcgagcgtg agcgtgaaat taaagcgttc
    11761 gtgccggaag agttctggga agtcgatgcc agcacgacca cgccatctgg tgaagcgttg
    11821 gcgttgcagg tgactcatca gaacgacaaa ccgttccgtc cggtcaacaa agaacaaact
    11881 caggctgcgg taagtctgct ggaaaaagcg cgctacagcg tgctggaacg tgaagacaaa
    11941 ccgacaacca gtaaacctgg cgctcctttt attacctcta cgctgcaaca agctgccagc
    12001 acccgtcttg gatttggcgt gaaaaaaacc atgatgatgg cgcagcgttt gtatgaagca
    12061 ggctatatca cttacatgcg taccgactcc actaacctga gtcaggacgc ggtaaatatg
    12121 gttcgcggtt atatcagcga taattttggt aagaaatatc tgccggaaag tccgaatcag
    12181 tacgccagca aagaaaactc acaggaagcg catgaagcga ttcgtccttc tgacgtcaat
    12241 gtgatggcgg aatcgctgaa ggatatggaa gcagatgcgc agaaactgta ccagttaatc
    12301 tggcgtcagt tcgtagcctg ccagatgacc ccagcgaaat atgactccac gacgctgacc
    12361 gttggtgcgg gcgatttccg cctgaaagca cgcggtcgta ttttgcgttt tgatggctgg
    12421 acaaaagtga tgcctgcgtt gcgtaaaggc gatgaagatc gcatcttacc agcagttaat
    12481 aaaggcgatg ctctgacgct cgttgaactt acaccagccc agcactttac caagccgcca
    12541 gcccgtttca gtgaagcatc gctggttaaa gagctggaaa aacgtggtat cggtcgtccg
    12601 tctacctatg cgtcgatcat ttcgaccatt caggatcgtg gctacgtgcg agtagaaaat
    12661 cgtcgtttct atgcggaaaa aatgggcgaa atcgtcaccg atcgccttga agaaaatttc
    12721 cgcgagttaa tgaactacga ctttaccgcg cagatggaaa acagcctcga ccaggtggca
    12781 aatcacgaag cagagtggaa agctgtactg gatcacttct tctcggattt cacccagcag
    12841 ttagataaag ctgaaaaaga tccggaagag ggtggtatgc gcccgaacca gatggttctg
    12901 accagcattg actgcccgac ttgtggtcgc aaaatgggga ttcgcacagc gagcaccggg
    12961 gtattccttg gctgttctgg ctatgcgctg ccgccgaaag agcgttgcaa aaccaccatt
    13021 aacctggtgc cggaaaacga agtgctgaac gtgctggaag gcgaagatgc tgaaaccaac
    13081 gcgctgcgcg caaaacgtcg ttgcccgaaa tgcggcacgg cgatggacag ctatctcatc
    13141 gatccgaaac gtaagttgca tgtctgtgg
//
LOCUS       DAMZET010000117        12914 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.117, whole genome shotgun sequence.
ACCESSION   DAMZET010000117 DAMZET010000000
VERSION     DAMZET010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.117"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..690
                     /gene="sapD"
                     /locus_tag="POB34_003359"
     CDS             <1..690
                     /gene="sapD"
                     /locus_tag="POB34_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HDI5920246.1"
                     /translation="LDPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGI
                     KDHKDAMRSFPYELTEGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTR
                     LNQNSNTTILLISHDLQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRA
                     IPDFGSAMPHKSRLNTLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLY
                     ACHFPLNMEKE"
     gene            692..1498
                     /gene="sapF"
                     /locus_tag="POB34_003360"
     CDS             692..1498
                     /gene="sapF"
                     /locus_tag="POB34_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HDI5920247.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTNLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            1566..1919
                     /locus_tag="POB34_003361"
     CDS             1566..1919
                     /locus_tag="POB34_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HDI5920248.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRHFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQSWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            2287..3075
                     /gene="fabI"
                     /locus_tag="POB34_003362"
     CDS             2287..3075
                     /gene="fabI"
                     /locus_tag="POB34_003362"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDI5920249.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            3220..4347
                     /locus_tag="POB34_003363"
     CDS             3220..4347
                     /locus_tag="POB34_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HDI5920250.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            4415..6349
                     /gene="rnb"
                     /locus_tag="POB34_003364"
     CDS             4415..6349
                     /gene="rnb"
                     /locus_tag="POB34_003364"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HDI5920251.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(6427..6531)
                     /locus_tag="POB34_003365"
     CDS             complement(6427..6531)
                     /locus_tag="POB34_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920252.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            6585..8570
                     /gene="pdeR"
                     /locus_tag="POB34_003366"
     CDS             6585..8570
                     /gene="pdeR"
                     /locus_tag="POB34_003366"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDI5920253.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVMRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            8717..8890
                     /locus_tag="POB34_003367"
     CDS             8717..8890
                     /locus_tag="POB34_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920254.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            8980..9729
                     /locus_tag="POB34_003368"
     CDS             8980..9729
                     /locus_tag="POB34_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDI5920255.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            9998..10216
                     /gene="osmB"
                     /locus_tag="POB34_003369"
     CDS             9998..10216
                     /gene="osmB"
                     /locus_tag="POB34_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HDI5920256.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(10342..10668)
                     /gene="yciH"
                     /locus_tag="POB34_003370"
     CDS             complement(10342..10668)
                     /gene="yciH"
                     /locus_tag="POB34_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HDI5920257.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(10668..11405)
                     /gene="pyrF"
                     /locus_tag="POB34_003371"
     CDS             complement(10668..11405)
                     /gene="pyrF"
                     /locus_tag="POB34_003371"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDI5920258.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(11598..12767)
                     /gene="lapB"
                     /locus_tag="POB34_003372"
     CDS             complement(11598..12767)
                     /gene="lapB"
                     /locus_tag="POB34_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HDI5920259.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(12774..>12914)
                     /gene="lapA"
                     /locus_tag="POB34_003373"
     CDS             complement(12774..>12914)
                     /gene="lapA"
                     /locus_tag="POB34_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HDI5920260.1"
                     /translation="WLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAA
                     KE"
BASE COUNT         3209 a         3285 c         3183 g         3237 t
ORIGIN      
        1 ctagatcctt cagaacgtgt gggtcgccag ttgatgcaaa acatcccagc ctggacctat
       61 aaaggccgtt ggtggcagcg ttttggctgg cgcaaacgcc gtgccattga actactgcac
      121 cgcgtgggga ttaaagatca caaagatgcg atgcgcagtt ttccctatga gttgaccgaa
      181 ggtgaatgtc aaaaagtgat gatagccatt gcactggcga atcaaccgcg tctgctgatt
      241 gctgacgaac cgacaaactc aatggagcca acaacccagg cgcaaatctt tcgcctgttg
      301 acgcgtctca accaaaacag caataccact attttgctta tcagccatga cttacaaatg
      361 cttagccaat gggcggataa aattaacgtg ctttactgcg gtcaaacagt ggaaaccgcg
      421 ccgagtaagg agttggtgac gatgccacat catccttata cccaggcgct gatccgcgcg
      481 ataccagact tcggcagcgc gatgccgcat aaaagtcgcc tcaatacgct gcccggcgct
      541 atcccactgc tggaacagtt accgattggt tgtcgtctgg ggccgcgttg cccgtacgca
      601 caacgagaat gcattgtgac gccacgtttg acgggggcga aaaatcatct ctatgcctgt
      661 catttcccgc tgaacatgga gaaagagtga gatgatcgaa acgctgcttg aagtgcgtaa
      721 tttaagtaaa acgttccgct accggaccgg ttggtttcgt cgtcagaccg tagaagcggt
      781 aaaacccttg agctttacgc tacgtgaagg ccagacactg gcgattattg gcgagaatgg
      841 ttcgggtaaa tccacgctgg caaaaatgct ggcgggaatg atagaaccta ccagcggcga
      901 gttattgatt gacgatcatc cactgcattt tggcgattat tccttccgca gtcagcgtat
      961 tcgcatgatt tttcaggatc cttcgacctc gttgaatccc cgtcaacgta tctcgcaaat
     1021 tctggatttt ccactgcgcc tgaacaccaa cctggaaccg gaacagcgtc gcaaacagat
     1081 tattgaaacg atgcgtatgg ttgggctgct accggatcac gtcagttact atccgcatat
     1141 gttggccccc gggcaaaaac agcgtctggg tctggcgcgc gcgctgatat tgcgcccaaa
     1201 agtcattatt gccgatgaag ccctcgcttc actggatatg tcgatgcgtt cgcagttgat
     1261 taatctgatg ctggaattac aggaaaaaca gggcatttcg tatatttatg ttacccagca
     1321 tatcggaatg atgaagcaca ttagcgacca ggtgctggtg atgcatcagg gcgaggttgt
     1381 cgaacgaggc agcaccgcgg atgtgctggc atcgccgctg catgaactca ccaaacggct
     1441 gattgccggg cactttggcg aagcattgac agcggatgcg tggcgtaaag atcgctaatt
     1501 ttctgaatcc tccttccaga ttatccacct cattctgaca agagattaaa tcttgtcaga
     1561 atgaggtgat ggataaaata aaatcaaatg cgcgtgattt aagacgcaat ctcactttgc
     1621 aggaacgaaa gctctggcga tatcttcgca gccgacattt tggtgatttc aaatttcgcc
     1681 gtcaacatcc ggtggggagc tacattctcg attttgcttg ctgctcggcg cgcgtagtcg
     1741 ttgagctgga tggtgggcag catgatttgg cagttgccta tgataccagg cgcactagct
     1801 ggcttgagtc gcagagctgg accgtgctgc gtttctggca taacgagatt gattgtaatg
     1861 aggaggcggt gctggagatt attctgcagg aactgaaccg ccggtcaccc tcaccctgaa
     1921 agggcgaggg ggccgaccga gccgaatagc tgttgtggtg aaaacatgga gacagtgctg
     1981 gagaatattc ggcaaggtct gaaccatccc agccatcgcc ataaaagggt taggggctgt
     2041 atgagcctgt ttgttgctgg ggtaacaata tttgcacaat atggtcccct cgcccctctg
     2101 gggagagggt tagggtaagg ggaaaagcgc ccaccctgcc gcagcctgct ccggtcggac
     2161 ctggcaacta tagctactca cagccaggtt gattataata accgtttatc tgttcgtact
     2221 gtttactaaa acgacgaatc gcctgatttt caggcacaac aagcatcaac aataaggatt
     2281 aaagctatgg gttttctttc cggtaagcgc attctggtaa ccggtgttgc cagcaaacta
     2341 tccatcgcct acggtatcgc tcaggcgatg caccgcgaag gagctgaact ggcattcacc
     2401 taccagaacg acaaactgaa aggccgcgta gaagaatttg ccgctcaatt gggttctgac
     2461 atcgttctgc agtgcgatgt tgcagaagat gccagcatcg acaccatgtt cgctgaactg
     2521 gggaaagttt ggccgaaatt tgacggtttc gtacactcta ttggttttgc acctggcgat
     2581 cagctggatg gtgactatgt taacgccgtt acccgtgaag gcttcaaaat tgcccacgac
     2641 atcagctcct acagcttcgt tgcaatggca aaagcttgcc gctccatgct gaatccgggt
     2701 tctgccctgc tgaccctttc ctaccttggc gctgagcgcg ctatcccgaa ctacaacgtt
     2761 atgggtctgg caaaagcgtc tctggaagcg aacgtgcgct atatggcgaa cgcgatgggt
     2821 ccggaaggtg tgcgtgttaa cgccatctct gctggtccga tccgtactct ggcggcctcc
     2881 ggtatcaaag acttccgcaa aatgctggct cattgcgaag ccgttacccc gattcgccgt
     2941 accgttacta ttgaagatgt gggtaactcc gcggcattcc tgtgctccga tctctctgcc
     3001 ggtatctccg gtgaagtggt ccacgttgac ggcggtttca gcatcgctgc aatgaacgaa
     3061 ctcgaattga aataatcatt ctgttggtaa atattgggcg gcgttctgcc gcccgttatc
     3121 tctgttatac ctttctgata tttgttatcg ccgatccgtc tttctcccct tcccgccttg
     3181 cgtcaggata acgatttcct ttacgaccaa ggagcgccca tggaacaacg ccacatcacc
     3241 ggcaaaagcc actggtatca tgaaacgcaa tccagtactg cggagtatga cgttctgcct
     3301 ctggtcccgg aagccgcaaa ggtcagcgat ccctttctgc tcgacgtgat ccttgatgaa
     3361 gaaacgctgg ctcccttcct ttcatggctg gtccctgcgc gcgttcttgc agtggaattg
     3421 ttccctgacc agcttaccgt gacccgttca cagaccttca ccgcttatga acgcttgtct
     3481 acggccctga cggttgctca ggtttgcggc gtccagcggt tatgtaacta ctattcggcg
     3541 cgacttacgc cgctccccgg gcctgattcc tccagggaaa gcaatcatag gttggcacaa
     3601 atcacgcaat atgcccgcca actggctagc tcgccttcta ttatcgacaa ccgatcgcgc
     3661 cagcatctga atgacgtcgg tcttactgcc tgggactgtg tgatcattaa ccaaatcatt
     3721 ggttttattg gctttcaggc gcggaccatt gcgacatttc aggcttatct cgggcacccg
     3781 gtacgctggt tacccggtct ggaaatacaa aactacgccg acgcgtcact gtttgctgat
     3841 gaatcaatac gctggcgaag cagctatgag gtggaaaaac tacctgaaga gtacacaaaa
     3901 agttcaacag cagaactttg ccaactggcc gaaacactct ctctccaccc tatttcactt
     3961 tcccttctcg aaaggttgtt aaacagtaca cgggttaata cacagccgga taataagctt
     4021 gcggcgttgt tatgcgcgcg gataaatggc agtcctgctt gttttgccgc ctgtatggat
     4081 tcatcaaatg aatataaaaa aatcagcccc cttctgcgca agggcgaaaa tgaaattaac
     4141 caatgggctg accgtcattc tgttgagcac gctaccgttc aggcgataca atggctgacc
     4201 cgagcacccg atcgctttag cgccgcccag ttcagccctt tactcgaaca cgaaaaatca
     4261 tcaacgcaga ttattaatct gctggtatgg agcgggctgt gtggctggat aaatcgctta
     4321 aaaatcgcgt tgggtgagac atattaacct tgccgcgtca gacagattcg cgtaaaactg
     4381 tcagccgctc taatggccac caaaatagac aattatgttt caggacaacc cgctgctagc
     4441 gcagcttaaa cagcaactgc attcccagac gccacgcgct gaaggggtgg taaaagccac
     4501 agaaaaaggc tttggcttcc tggaagtcga cgcgcaaaaa agttatttca ttccgccgcc
     4561 gcagatgaaa aaagtcatgc atggcgaccg aattatcgcg gtgatccaca gtgaaaaaga
     4621 acgtgaatcc gcagagccag aagaactggt tgaaccgttc ctgactcgtt tcgtgggtaa
     4681 agttcagggc aaaaatgacc gtctggccat cgttcctgat catccactct taaaagacgc
     4741 cattccttgc cgcgcagccc gtggcctgaa ccacgagttt aaagaaggcg actgggcggt
     4801 tgccgaaatg cgccgtcatc cgctgaaagg cgatcgttct ttctatgctg aactgacaca
     4861 atacatcact tttggtgacg accactttgt accgtggtgg gttacccttg cgcgccataa
     4921 tctggaaaaa gaagcaccag acggcgtcgc taccgaaatg ctcgatgaag gtctggttcg
     4981 tgaagatctg accgcgctgg attttgtcac catcgacagt gccagcacag aagatatgga
     5041 tgacgccctt ttcgctaagg cgttgccgga tgacaaactt cagctgattg tggcgattgc
     5101 cgatccaacc gcgtggattg ctgaaggcag caagctggac aaagccgcga aaattcgcgc
     5161 attcaccaac tatctgcctg gcttcaacat ccctatgctg cctcgcgagc tttctgacga
     5221 tctctgctca ctgcgcgcca atgaagtccg cccggtactg gcatgccgca tgacgctctc
     5281 cgctgatggc accattgaag ataatatcga attctttgcc gccaccatcg aatccaaagc
     5341 aaagctggtg tatgaccagg tttctgactg gctggaaaat accggtgact ggcagcctga
     5401 aagtgaagca attgccgaac aagtccgttt gctagcgcaa atttgccaac gccgcggcga
     5461 gtggcgtcat aaccacgcac tggtgtttaa agatcgcccg gattaccgct ttattctcgg
     5521 tgaaaaaggt gaagtgctgg atatcgtcgc cgagcctcgt cgcattgcca accgtatcgt
     5581 cgaagaagcg atgattgccg ctaacatttg tgcggcccgc gtactgcgcg ataagctcgg
     5641 ttttggcatc tataacgtgc atatgggctt tgatccggcg aatgccgacg cgctggcagc
     5701 gttgctgaaa acgcacggtc tgcatgtcga tgccgaagaa gtgctcacgc tggacggttt
     5761 ctgcaaactg cgtcgtgaac tggacgcgca accaactggt ttcctcgaca gccgcattcg
     5821 tcgcttccag tcatttgctg aaattagcac tgaacccggt cctcactttg gcctcggtct
     5881 ggaagcatac gccacctgga cttcgccgat ccgtaaatat ggcgacatga tcaaccaccg
     5941 tctgctgaaa gcggttatca aaggcgaaac tgcgacgcgc ccacaggatg aaatcactgt
     6001 ccaaatggcc gagcgtcgcc gtctcaaccg gatggcagaa cgtgatgttg gtgactggtt
     6061 atacgcacgc ttcctgaaag acaaagccgg gaccgacacc cgtttcgcag cggaaattgt
     6121 cgatatcagc cgtggcggca tgcgtgttcg tttggttgat aacggcgcta tcgcctttat
     6181 tccggcacct ttcttacacg ctgtgcgcga tgaactggtt tgcagccagg aaaacggcac
     6241 tgtacaaatt aaaggtgaaa cggtttacaa agtgactgac gttattgacg tcactattgc
     6301 cgaagtccgc atggaaaccc gcagcattat tgcgcgcccg gtcgcgtaat ctcctttcac
     6361 ggcccattcc tcatggatgg gccgtttatt tccccgctct accttcgtta tatccaggca
     6421 gattttttaa ctgactttcg tttgaaaact ggcgtttttc caaaactgga ttacaaataa
     6481 atatataaaa aaaacaatgc gtttagccgc cgttaatttc atgtaagtca tgttgttcct
     6541 tttatttaac cgcggactcc gcagttaacc ggaggatatg catcatgaaa accgttaggg
     6601 agtccacaac gttgtacaac tttctcggat cgcacaatcc atactggcgg ttgacggaaa
     6661 gcagcgatgt tatgcgcttt tctaccaccg aaaccacaga acctgatcgt actttgcagt
     6721 tatctgccga acaggctgct cgcatcaggg aaatgacggt catcacctcc agcctgatga
     6781 tgagtctgac cgtcgatgag agcgatcttt ctgtgcatct ggtaggacga aaaatcaata
     6841 aacgggaatg ggctggcaac gcgtctgcat ggcatgacac accggcggtt gctcgtgatt
     6901 tatcacacgg gctttccttt gctgagcagg tagtttctga agcacattcc gcaatagtca
     6961 ttctcgacag ccgggggaat attcaacgct tcaatcggtt atgtgaagat tacacagggt
     7021 tgaaagaaca cgacgtcatt gggcaaagcg tgtttaaact gtttatgagc cgtcgtgaag
     7081 ctgcggcatc caggcgcaat aaccgtgtat tttttcgaag cggcaatgca tatgaagtcg
     7141 aactgtggat accaacatgt aaaggccagc ggctgtttct gtttcgcaat aaatttgtcc
     7201 acagcggcag tggcaaaaac gagatttttt taatctgttc cggcaccgac attaccgaag
     7261 agcgccgcgc tcaggagcga ctgcgtattc tggcaaatac cgacagtatc accggactgc
     7321 cgaatcgtaa cgcaatgcag gatttaatcg atcacgctat taatcatgca gataacaata
     7381 aagttggggt tgtgtatctt gatttggata atttcaaaaa ggtcaacgac gcctatgggc
     7441 atttgtttgg tgaccagtta ttacgcgacg tgtcattggc tattttaagc tgtctcgaac
     7501 atgaccaggt gttggcgcgt ccaggtgggg atgagtttct ggtactggca tccaacacct
     7561 cacaaagcgc gctggaagca atggcatcac gaattttgac ccgcttacgg ctcccctttc
     7621 gcattggttt aattgaagtt tataccagct gttcagtagg tattgcactc tctcccgaac
     7681 atggttcaga cagcacggct attattcgtc acgccgacac agcaatgtac acagcgaagg
     7741 aaggcggacg aggacaattt tgcgttttta ccccagaaat gaatcaacgg gtatttgaat
     7801 atctctggct ggataccaac ttgcgtaaag cactggaaaa cgatcagttg gttattcact
     7861 atcaaccgaa aatcacctgg cgtggcgaag tgcgcagtct ggaagcacta gtacgttggc
     7921 agtcacctga acgtgggttg attccaccgt tggacttcat ttcctacgcc gaagagtcag
     7981 ggctaattgt gcctttaggc cgttgggtga ttctcgatgt cgtacgccag gtggcaaagt
     8041 ggcgggataa aggcataaac ctgcgagtgg cggtaaatat ttctgcacgt cagctcgccg
     8101 atcaaaccat tttcaccgcc ctgaaacagg ttctccagga actcaatttt gaatactgcc
     8161 ctatagatgt tgaactgaca gagagttgtc tgattgagaa tgatgaactg gcactgtctg
     8221 ttattcaaca atttagccaa ctaggtgcgc aagtgcatct ggatgatttt ggtaccggct
     8281 actcttcact ttcgcaactg gcgcgctttc cgatcgatgc catcaaactt gaccaggttt
     8341 ttgttcgaga tattcacaaa caacctgtct cgcagtcact ggtccgggcg atcgtcgctg
     8401 tggcccaggc attgaatctt caggtgatcg ccgaaggtgt agagagtgca aaggaagatg
     8461 cttttttaac caagaacggg atcaatgagc ggcaaggatt tttgtttgcc aaaccgatgc
     8521 ccgccgtcgc cttcgaacgc tggtataaac gctatctgaa gcgcgcataa gtagatgaat
     8581 atatatactt agcgctatat cccgtcatta tatatcgtat tcctggatga taattagccg
     8641 ttgctgttaa acacttctct tttagcggca atttttgcat tattcatttt ccacttttgc
     8701 tataaggatg tccttgatgt ctgaatttga tgcccagcgc gttgccgaac gtattgatat
     8761 tgtgttagat atccttgtgg caggcgatta ccactctgcg atccataatc tcgagatact
     8821 taaagcagaa ctcttgcgcc aggttgctga atctacgcca gacatcccga aggcaccgtg
     8881 ggagatttaa caatcgtgcc ttcaggtttt cctatttgag gatgcccggc gcgtcagtat
     8941 ttttttattt agtattataa cgttataaga attacagcga tgaattcccg acaacaaact
     9001 attctacaga tggtcattga ccagggtcag gttagcgtaa ccgatctggc aaaagccact
     9061 ggagtttctg aagttaccat tcgccaggat ctcaacaccc tcgaaaaact gagttacctc
     9121 cgccgtgcac atggctttgc agtttcgctt gatagtgatg acgtcgaaac ccgtatgatg
     9181 agcaactata cgctgaagcg tgaactcgcc gagtttgccg cgtcactggt tcaaccgggc
     9241 gaaaccatct ttatcgaaaa tggtagcagc aatgccctgc tcgcccggac tctgggggag
     9301 cagaagaaaa atgtcactat catcacggtc agcagctaca tcgcgcattt gctgaaagac
     9361 gcgccttgtg aagtcatttt gctcggtggc gtgtaccaga aaaaaagcga aagtatggtt
     9421 ggcccgttga cacgccagtg catccagcag gtgcatttca gcaaagcatt tattggtatt
     9481 gatggctggc aacctgaaac tggattcacc ggtcgcgata tgatgcgtac cgatgtggtc
     9541 aatgccgtgc tggaaaaaga gtgcgaagcg atagtcctga ctgacagctc gaaatttggt
     9601 gctgtacatt catactccat cggtcccgtt gagcgattca atcgcgtgat taccgattcg
     9661 aaaatacgcg ccagcgatct gatgcatctt gagcacagta aactcaccgt tcacgtcgtt
     9721 gacatttaac catttccctt ctctacggat gatttgcagt ttggcaaatc atccgctcta
     9781 agatgattcc tggttgataa ttaagactat ttacctgtta ttaacacgct taagatataa
     9841 aattattatc agcgatataa caggaagtca ttatcacctg cgtgatataa ccctgcgcgc
     9901 gagcagattt cacggaataa tttcaccagg cttattctta tctattatag ttatagagag
     9961 cttacttccg tgaatcataa attcaggaga gagtattatg tttgtaacga gcaaaaaaat
    10021 gaccgcggct gttctggcaa ttactttggc aatgtctctg agtgcctgtt ctaactggtc
    10081 taaacgggac cgcaacaccg caatcggcgc gggtgcaggg gcattaggcg gtgcagtact
    10141 gaccgatggc agtacgttgg gtacattagg tggtgcagct gtcggtggtg ttattggtca
    10201 ccaggtcggt aaataatcgt aaataaatgt cgattatcga ctcgcaatta taatacgctt
    10261 gattctgttc agtacgaaat aactgaataa tacgcacagt aaaatattcg aaagccacgg
    10321 atatatccgt ggctttttat gttaaccgcc tgcgagtttt accttcatcc ctttcgcttc
    10381 aagtagtgat tttaataaat cacgcttatc gccctggatt tcaataactc catctttaac
    10441 tgctccgccg cagccgcatt ttttcttcag ttccgctgca agttttgtca gttcggcatc
    10501 atcgagatcg acaccggtaa tcaggcaaac gcccttacct ttacgtccac tggtctgacg
    10561 ctgaatacgc accacaccgt cgcctttagg gcgtacaggg gccgctttgg gctcatcaat
    10621 acgtccggtt tccgttgagt agaccagacg gctgttggaa tcactcatca tgcactccgc
    10681 tgtaaagagg cgttgatcgc tttcagcgtc tgcgctggat ctaccgattg cgttaccggg
    10741 cgaccaatca ccatataatc aacaccagcc gacaacgcct gttctggcgt cataatgcgg
    10801 cgctggtcac cagcttcact cccctgcgga cgaatgcccg gcgtaaccag tttgaactcc
    10861 tgaccgaata cctgtttaaa gcgcacagct tcctgagcag aacacaccac accatcaagg
    10921 ccacattttt gcgtcagtgc cgccagacgt tctgcataat ctgcaggtga cagtgtcatg
    10981 ccaagatcga ccaggtcgct ggcttccatg ctggtcaaca ctgtcacagc aatcaaaagc
    11041 ggtgcatctt tgccaaacgg aaccagtgcc tcacgcgctg cggtcatcat acgcgcccca
    11101 ccagaggcat gaacattcac catccacacg cctaagtcag ctgcagcagc gacagcgtgc
    11161 gcagcagtgt taggaatatc gtggaatttc aggtcaagaa agatatcaaa accacgctgt
    11221 tgaagttcgc gcacaaactg tggcccaaac aatgtaaaca tctctttgcc gaccttcaga
    11281 cgacaatcgc gtgggtcgat cttgtcgaca aaggacagcg cgtcatcacg attatgataa
    11341 tcaagggcaa caaccacagg agaattcgta acagcgcggg aagaagatga agcagttaac
    11401 gtcatgacca gaccttcttg atgatgggca ccggaagtgg cgcgaaacag gtaaacggcg
    11461 agcattctac ccgtggaaat aaaaaattaa cagttgcgat ttttcccctg gcaggctttt
    11521 tctgcgccgt cgttgccaga gcacaatgga acatcaataa ctagtatgtt gtaactaaag
    11581 taggattttt ttaaaaatta caggccatca agaccgcgaa tcggtttaat ggttgaccag
    11641 gcccgacaag acggacaatg ccagtagagg gtgtatgcgg taaaaccaca tttctggcag
    11701 cgataacgag gcttactacg taccttttcg ccaaccatgt cacgcagcac catcaggctc
    11761 tctttggcac gcccttcttc cgcttcattt aagtggtaat ccattaactt atggaacaca
    11821 cgcatggtcg gatgacgctg aagctggcgc gtaatataga cctgtgcggc ctcactaccg
    11881 tcgcgcgctt cgatgatatc agcaagcatc aattcagcat cggcaccggt gttctcttcc
    11941 accgcgcgtt gcaggaattc tgcccattcg gcagttttac ccaactgctg atagcaggtt
    12001 tgcaacattt ccagcgtttc gctgaccagt tctctgtcct gggatatcac gcgttgcaaa
    12061 ctttcgacgg ctttggcgta ttctcctttc gccataaaca cgcgtcccat catgatggat
    12121 acgcgggcgc tgtttttatc tgccgccgcc ccttttttta gcaaggtcat ggcacgatcg
    12181 agatcgtcgc tggccatatg ctgcagggct aactcacagt agaaatgggc aatttcgacg
    12241 cgctgtttat ctttacccag cttcaccagg cgttcggcaa catcaattgc tttctgccac
    12301 tcactggtag cctggtagat ttgtagcaac tgttgcagag cgccaatgcg gaagtcagtt
    12361 tcatcggtca gctgattgaa catgtcttcc gcgcggtcgt ataacccggc ggccatgtaa
    12421 tcacgcccta gttgttgaat cgccaacaga cgctgttcat aggtcagcga ggcgctttcc
    12481 attagggtct gatggatgcg aatagcgcga tcaacttcgc cacgcgaacg gaacaggttt
    12541 ccgagcgtaa ggtgggcttc aacggtgcct gtatcctctt taagcatatc gagaaacagg
    12601 tctaccgctt tatcctgttg attactaagc aggaagttaa cccccgctac gtaatcacgc
    12661 gacaagcggt tggcttcatc ttgcttgttt tgttgcgcac ttctgcggcc catataccag
    12721 ccataggcag cggctacagg caaaagcaga aacaacaact ccagcataga aagttattcc
    12781 ttcgccgctg acgagtgcgg cactacagcc acgtcagtcg cgggtgaaag ctggttttcc
    12841 agtcgcttta ttttacgttc agcgcgcgcc agggaaacac gaactcgcag ccagaacagg
    12901 ccacaaatca acca
//
LOCUS       DAMZET010000118        12883 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.118, whole genome shotgun sequence.
ACCESSION   DAMZET010000118 DAMZET010000000
VERSION     DAMZET010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.118"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..242)
                     /locus_tag="POB34_003374"
     CDS             complement(<1..242)
                     /locus_tag="POB34_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDI5920261.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(453..1850)
                     /gene="ccp"
                     /locus_tag="POB34_003375"
     CDS             complement(453..1850)
                     /gene="ccp"
                     /locus_tag="POB34_003375"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HDI5920262.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2255..3904
                     /locus_tag="POB34_003376"
     CDS             2255..3904
                     /locus_tag="POB34_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDI5920263.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3955..4557)
                     /locus_tag="POB34_003377"
     CDS             complement(3955..4557)
                     /locus_tag="POB34_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI5920264.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            5077..5976
                     /locus_tag="POB34_003378"
     CDS             5077..5976
                     /locus_tag="POB34_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI5920265.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            6025..7038
                     /gene="yhjD"
                     /locus_tag="POB34_003379"
     CDS             6025..7038
                     /gene="yhjD"
                     /locus_tag="POB34_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HDI5920266.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7449..8771
                     /locus_tag="POB34_003380"
     CDS             7449..8771
                     /locus_tag="POB34_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HDI5920267.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(9005..11065)
                     /locus_tag="POB34_003381"
     CDS             complement(9005..11065)
                     /locus_tag="POB34_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDI5920268.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(11135..11902)
                     /gene="pdeH"
                     /locus_tag="POB34_003382"
     CDS             complement(11135..11902)
                     /gene="pdeH"
                     /locus_tag="POB34_003382"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HDI5920269.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVAHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            12134..>12883
                     /gene="kdgK"
                     /locus_tag="POB34_003383"
     CDS             12134..>12883
                     /gene="kdgK"
                     /locus_tag="POB34_003383"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HDI5920270.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVK"
BASE COUNT         3132 a         3446 c         3118 g         3187 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tacaggctta atttaaataa caaaatccta agcagaaaaa cgacttttta
      361 taatataaac atttaacatg ataatattaa aaacagactt tattatcgta ataaaaatcg
      421 cccaaacatt cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
      481 tacaccccgt tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc
      541 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      601 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
      661 aatttatcac gctcttcttt agtcacattc atacgaccaa tatccgccgc cgtaatttcc
      721 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccgcc gagaataata
      781 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
      841 gccgtcagcg cattttcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt
      901 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga
      961 taaacttcga ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg
     1021 tcccaggatt tcgacgccat ttcaatcggg ttcaacggcg gtccacctgc ctgatcctgc
     1081 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
     1141 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg
     1201 ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg
     1261 ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg catcggtagg cagtttttgc
     1321 gggatgggct gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat
     1381 tccgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1441 cccgcccagt gtagcgcggt ataacgcgtt ggtggcatag tttcatactg catcacccat
     1501 tcaattttat tcagatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg
     1561 gcctcgaggt taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg
     1621 ccaggaatat aataataggc gggtaattct gccgaaggtg tgtggcaata gtcgcatcct
     1681 ttttcgcgga gaaagcctaa aaccttatta ttttcactga cagcagatgc ctgaacatcg
     1741 gcctgtttac tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa
     1801 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     1861 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     1921 agacaaagcc atcataaaaa acagggagat acgctttttg ataggaatca atcaattaca
     1981 tgaatataaa ctatcaataa gatagcctgt gcctattacc cagatcagga atggcatatc
     2041 atgattgcgg cgagagcagg attgaatgtt ctgaaaatga aaaacaacac gaaatccgta
     2101 tttttataaa ttccatggat attagtgcgt tatatttttt gacaggttaa taaacgtgat
     2161 ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg
     2221 ataattagcg acgaatttcg gaggttggat ccttatgctc aatcagaaaa ttcaaaaccc
     2281 taatccagac gaactgatga tcgaagtcga tctctgctat gagctggacc cgtatgaatt
     2341 aaaactggat gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc
     2401 ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg ttcgagcatg ttcagtcggc
     2461 gaaaattttc cccgacagta aaacctttcc cgactgcgca cccaaaatgg acccgctgga
     2521 tattttaatc cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt
     2581 tgaaaatcac ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa
     2641 ttccctgaaa gagcatatcg accagctgtg gccggtgcta acccgcgaac cacaggatca
     2701 cattccgtgg tcttctctgc tggcgctacc gcagtcatat attgtcccgg gcggccgttt
     2761 tagcgaaacc tactattggg attcctattt caccatgctg gggctggcgg aaagtggtcg
     2821 ggaagatttg ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actatggtca
     2881 catccccaac ggcaaccgca cctattattt gagccgatcg caaccaccgg tttttgcgct
     2941 gatggtggag ttgtttgaag aagatggtgt acgcggtgcg cgccgctatc tcgaccacct
     3001 taaaatggaa tatgccttct ggatggacgg tgcagaatcg ttgatcccta atcaggccta
     3061 tcgccatgtt gtgcggatgc cggacggatc gctgctcaac cgttattggg acgatcgcga
     3121 cacgccgcgt gacgaatcct ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc
     3181 gcccaacgag gtgtaccgcg atttacgcgc gggagcggcc tcaggttggg attactcttc
     3241 ccgttggctg cgtgatactg gtcgtctggc gagcattcgt accacccagt tcatccccat
     3301 cgatctgaat gccttcctgt ttaaactgga gagcgccatc gccaacatct cggcgctgaa
     3361 aggcgagaaa gagacagaag cgctgttccg ccagaaggcc agtgcccgtc gcgatgcggt
     3421 aaaccgttac ctctgggatg atgaaaacgg catctaccgc gattacgact ggcgacgcga
     3481 acaactggcg ctgttttccg ctgccgccat tgtgccgctc tatgtcggca tggcgaacca
     3541 tgaacaggcc gatcgtctgg caaacgccgt acgcagccgg ttactgacac ctggcgggat
     3601 tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa cccaatggct gggcaccgtt
     3661 acaatggatg gcaattcagg gatttaaaat gtatggcgat gaccttctgg gtgatgaaat
     3721 cgcgcgcagc tggctgaaaa cggtgaatca gttctatctg gaacagcaca aaatgatcga
     3781 gaaataccat attgccgatg gtgttccccg cgaaggcggc ggtggcgagt atccgttgca
     3841 ggatgggttt ggctggacta acggtgtggt acgccgttta attggtttgt acggcgaacc
     3901 ataatatttt tacagccagc cgctaacttc ctgctggctg taaaattatc ctcttcagga
     3961 ggagatattt aacatcattg ccgcctgggt gcgatttttc acttccagac ggcgatacag
     4021 ggattccaga tgcgctttta ccgttccggt actgatattc agcgctctgc cgatctcctt
     4081 atttgattcg cccgccgcta acatggttaa aatctcccgc tggcgggcgc ttaacgattt
     4141 gagatcttta atgtcctttt ccggcgtcgt ccgccagtct ccaggcagaa acatcatccc
     4201 catcgccgca ctatttaccg ccaacgcaaa tgtctcgacg gttgaatcac gaggcacaat
     4261 ggccagcaca ttaaaatgaa taacttcctg taaccaccgt ttattgcaat ccgtcgccgt
     4321 gattaacacc ttaacctcag gaaactgcac cacggttttt tgcagcaacc agtagcaaaa
     4381 ctcaccatcc tgatcgccat cgagcataac taaggcttca gggtaacttt ccagcttttg
     4441 ccataactcg tctgcctgac tggccccctg aatactcact cctggaatac gctgctgtaa
     4501 actgattttc attccatgaa taaatattga ctgcctgtca aacatgacta tttgcataac
     4561 tgaatctcca cctgaatacg ttaaaaagac ttaagtagtg gaagggtatt acccgcgaga
     4621 aaaaataaga attcgccatt tggcggtggc cattctacag agatgacgcg tagaaaatag
     4681 ttaccgatat aaatagttac agctaaacgc ctgaaattac atgtcgaggg cactatttaa
     4741 aacaattttg aggatttcct tatattggtg gttagtacgc atgcaattaa aaatgaaact
     4801 ccgcgaccac aagcaaaaat aacaaacggc aatgagacaa aaataagcac aaatagccaa
     4861 cacgtcctct attcacttta aagggaatcg ctgaaaaata cgctctgttt agggggattc
     4921 acctttctca gaaagctatt ccgccctttt cctgctgaga aatcgccaca ttcgtcatga
     4981 caacattgtg aaacccggca ttagatgtta gaaaaaacaa taaacaatgc gatatgcgcg
     5041 ttactgtttc gctgtgaaca acaatcggtc aaagaaatgg ataaaattca cgcaatgcag
     5101 ttgttcatca aagtcgcgga gctggaaagt ttttcccgcg cggcggattt ctttgctttg
     5161 ccaaaaggaa gtgtttcgcg ccagatacag gcactggaac atcaacttgg cacccagctt
     5221 ctccagcgca ccacgcgacg ggtcaaactc acgccagaag gcatgaccta ttatcaacga
     5281 gcaaaagatg tgttgagtaa tctcagcgaa ctggacggtc tgtttcaaca ggatgccacc
     5341 agtatcagcg gtaaattacg catcgacatc ccgccaggaa tcgcgaaaag cctgttactg
     5401 ccgcgcctgt cggaatttct ctatctgcat ccgggaattg agctggaact gagtagccat
     5461 gaccgtccgg tagatattct tcatgatggt tttgattgcg tgatacgcac tggcgcgtta
     5521 ccggaagatg gcgttatcgc ccgtcccctc ggcaaactga ccatggtcaa ctgtgccagt
     5581 ccgcactatc tgacgcgctt tggttatccg caaagccccg atgatctgac ttcacacgca
     5641 atagtgcgtt atacaccgca cctgggtgta catccgttag gttttgaggt tgccagcgtt
     5701 aatggcgtcc agtggtttaa gtctggcggc atgttgacgg taaacagtag cgaaaactat
     5761 ctcgccgccg gtcttgccgg tctggggatt attcagatcc cgcgcattgc cgtgcgcgaa
     5821 gccctgcgtg ccgggcggct tattgaagtg ttgccaggct accgtgccga gccgctctcc
     5881 ctttcgctgg tttacccgca gcgtcgggag ctttcccggc gtgtaaacct gtttatgcag
     5941 tggctggctg gcgtaatgaa agagcacctg gactgaccga ctatactttt taagaatgac
     6001 cacgacagaa ggacaaaaga gcggatgacg caggaaaacg agatcaaacg tcccacccag
     6061 gatctggagc acgagccgat taagcagttg gataatagcg agaaaggcgg caaagttagc
     6121 caggcgctgg aaaccgtcac caccactgcc gaaaaagtcc aacgccaacc ggttattgcg
     6181 cacctgattc gcgcgacaga acgctttaac gatcggctgg gtaaccagtt tggtgcggct
     6241 atcacctatt tctcattttt atcgatgatc ccgattttga tggtgtcgtt tgccgcaggg
     6301 ggctttgtgc tggcctccca tccgatgttg ctacaggata tcttcgacaa aattctgcaa
     6361 aacatcagcg atccgacgct agccgccacg ttgaaaaaca ccatcaacac cgccgttcag
     6421 cagcgtacga ctgtagggct tgtcggcctg gcggtggcgc tttattctgg catcaactgg
     6481 atgggtaacc tgcgtgaagc gattcgtgcc cagtcgcgcg atgtctggga acgctcgccg
     6541 caagatcagg agaagttctg ggtaaaatat ctgcgtgatt ttatttcact gattggtttg
     6601 ttaattgcgc tgattgtgac gctgtcgatc acctcggttg ccggttcggc gcagcaaatg
     6661 attattagcg ccctgcacct gaacagcatt gagtggctga aaccgacgtg gcggttgatt
     6721 ggcctggcga tttccatctt cgccaactat ctgcttttct tctggatctt ctggcggttg
     6781 ccgcgccacc gtccgcgcaa aaaagccctg attcgcggaa catttctggc tgctattggt
     6841 tttgaagtga ttaaaatcgt gatgacctac accttgccat cgctgatgaa atccccctct
     6901 ggcgcagcat ttggttccgt gctggggctg atggcatttt tctacttctt cgcccgtttg
     6961 acgctgtttt gcgcggcgtg gattgccacc gccgaatata aagacgaccc gcgaatgcca
     7021 gggaaaacgc agccttaaaa taggccggat gcggcgtaaa cgccttatcc ggcatacgat
     7081 tttattcggc ctggctcccc gtaggccgga taagatgcgc cagcatcgca tccggctata
     7141 atgcgcacat aacctcttga aactcttccc cagagcctct tcagccatct atttgggagc
     7201 aaacaatttc attccaactc ataaccccag catataaatc cagttggtaa cttttattta
     7261 acctgaaacc agttttatcc actatttata aaattatgtg aagcatttca tagaagaaaa
     7321 atcactggcc taaacattat cccctttttg cctggttttt gaccatttcc gcgatttgtt
     7381 acacattgaa atatcacttt tgctgtgcgt aatatggcta ttcgttagcc aaaaaataag
     7441 aaaagattat gcaagcaaca gccacaacac tcgaccacga gcaagaatac acgccaatca
     7501 actcgcgtaa taaagtcctt gtcgcctctc tcattggcac ggccattgag ttcttcgact
     7561 tttacattta cgccactgcg gccgttattg tgtttccgca tatcttcttc ccgcagggcg
     7621 atccaacggc agcaacgcta cagtcgctcg ccaccttcgc catcgccttc gtcgcgcgcc
     7681 ccattggctc tgccgttttt ggtcattttg gcgatcgcgt tgggcgtaaa gcgacgctgg
     7741 tcgcctcgtt gctaacgatg gggatttcga ccgtggtgat tggtctgctg ccgggctatg
     7801 ccacgattgg tattttcgcc ccgctgctgc tggcgctggc tcgatttggt cagggtctgg
     7861 gcttaggcgg tgaatggggc ggcgcggcgc tgctggcgac tgaaaacgcc ccaccgcgca
     7921 aacgtgcact gtatggctcc ttcccacagc tgggcgcacc gattggcttc ttctttgcca
     7981 acggcacttt cttgctgctt tcctggctac tgaccgacga gcagtttatg agctggggct
     8041 ggcgtgtgcc atttatcttc tcggcggtgc tggtcattat cggcctgtat gttcgcgtgt
     8101 cgctgcatga gtcgccggtg tttgagaaag tcgctaaagc gaaaaaacag gtgaagatcc
     8161 cgctgggtac gctgctgacc aaacatgttc gcgtaaccgt actgggtacg ttcattatgc
     8221 tggcaaccta tacgctgttt tacatcatga cggtctactc tatgaccttc agtaccgccg
     8281 ccgcgccagt tgggcttggc ctgccgcgta acgaagtgtt gtggatgctg atgatggcag
     8341 ttattggttt tggcgtgatg gtgccagtcg ccggattact ggctgatgcc tttggtcgcc
     8401 gtaaaagcat ggtaatcatc accacgctga tcatcctgtt cgcgctgttc gcctttaacc
     8461 cgctgctcgg ttctggcaac ccgattctgg tttttgcctt cctgctgctg gggttaagtc
     8521 tgatgggact gaccttcggg ccgatgggcg cgctgttacc agagctgttc ccgacagaag
     8581 tgcgttacac cggagcatcg ttctcttaca acgtagcgtc gattctcggg gcttccgttg
     8641 caccatatat cgcagcctgg ttgcaggcta actacgggtt aggtgcggtg gggttatatc
     8701 tggcggcgat ggctggcttg acgttaatcg ccctgctgct gacccatgag acgcgacatc
     8761 agtcgttgta agtatcgtgt gggcagccac cctcacccca gccctctccc tgaaagggag
     8821 agggagaaaa gcggagcgag atgttatatc gcgaacataa cagccctccc ccagaaagtg
     8881 aacggaaaag cggtactcgt tgtttcaaca cgcacaaaac agtcccctcg cccctttggg
     8941 gagagggtta gggtgagggg aacacgctgc tcccgaactc accagcaaac acaatccaac
     9001 ctcatcactt cttcatctgc gacaatatcg tccgacactg attagcctcc ccttcggaag
     9061 gtgagatcag tgccagtaac gccgccgccg gtgttaccag cgttgccagt gccgcagcaa
     9121 cagcaccacg ggcaatcaac ggcccggctt tcaccccagc ctgcggattt ttaaacgtcc
     9181 cacgcacata cagcggcgaa cgcagtgtga taatccgaat ccctttactc tccggatcaa
     9241 tagtcaaatc cagctgttcc gaagcaaaac ttgccgtgcc ggtaacatta atcaacgcgt
     9301 tctcagtatc gaaagcaaaa atctgcgggc gcgccacgcc gttggcaata ttcagattcg
     9361 ccgccgcgca gttcacccgc acctcatcgt caccaaatat cgcaccgaca atgtagttgc
     9421 cgacattcag cccaacaatc tccatcaggt tgcggctcac cagcccgtca ttcatcaaca
     9481 gtttcaggtt gccgttactg ttgcctaaaa gtgctgccac cgagttaccg ctaccgcgta
     9541 gttccgcgtc accgttcatt tcccccagcg tcttctgcat cagttccaca tcgggcatca
     9601 gttctttcag tttcagtcga cgagcctgaa tatctgcccg cccctgcatc ggctttttat
     9661 cgccttccag atgaatattc gccgcaatgc tgccgcccgc catgccaaat ttcagcggtt
     9721 gcaggcgcag gtcagcattt ttgaggatga tatgagtaga aagatcgcta atcggcaggc
     9781 tactgccatg ctcaatgcgc cgccctttga agcgaacatc ggcatccata acgtcccatt
     9841 tatcggtttc gaagcggtca taaggcagca ctttgcccgc aggctgaacg cttttttcgc
     9901 ccttcttctg ttcagaccgt ttcgactttt ctgccccttt cccggagtca acgccaatta
     9961 acggtcccag gtccgccagc cgcaattgcc gcgactcgac atcaccttcc agttttggtc
    10021 gtggctttcc ggtggtgtag accagagaac cgtggatatc gctatcgcca attcgcccat
    10081 taaaaccgcg ataatcaaag accgacgatt tttcagtgtc gattttcgct accagccgac
    10141 catccgtttc aaacggcggg gtatcgggca gcagaacgcc cgtcagttca tagagatcac
    10201 ccagtgaatc gccagaaaat ttaagccgta aatcgacacc gcccatcttc attgggtcat
    10261 tcacgacgcc atcaaaagca acacgggtgt taccagagcg gaaatcagcc tgtaccggaa
    10321 acggcgtccc ttcgccacgc agcgccagca taccgcctat ttttcccgta cccgtgagcg
    10381 gttcaccgtt ataacgtccc tgcgccttca ggccaaaaac gtaatcgccc accttttctt
    10441 tatccgcttt acctttcgat ccagtaactt cgctgaacgg cagcggcttg cctaacggat
    10501 caacaaaaat ctccagatcc gctttgctta ctttgtcatc aatggcgatc cgcccttgat
    10561 cgaaaagaat attatccagc cgaaacgacc atgccgacgg ctttgcattc gcgtctttgt
    10621 tatcatcgtt ggcgagatta aacgtccagt tattgttctt ttcggagagg cgaatcaggc
    10681 gcgcgtcggg cttttcgagc ttgatccacg gcagccagac cgttttggtc agcagcgcca
    10741 gcggggccag cgttgcctct acgcgtggca aatgcaccat cgtgacttcg ggaatatccg
    10801 gtggattgcc aagaatgatg tcttccgcgt gtacatgggg ccacggcacc cagctgcgcc
    10861 agcctgtttc ttgcttttgc cgctcccaca ccacgcccag atcgccacgg atagcgaacg
    10921 gacgattcaa ctccgcagag actttctggt tgatggtcgg tttgagtcga ttccagtcaa
    10981 aagttgcaat caaaatgatc gccacgacaa tcaacaacaa gaaagcccct gaaatcgcag
    11041 cggttatttt gcctgccttg ctcatcgttc gcttcctcat attcttcctg tgccagtcct
    11101 aaagatagtc cagccaggcg gaaaatgagg cagcttatag cgccagaacc gccgtattca
    11161 gcgtttctat cggtgccggg cgtgaaagaa accagccttg tgcggcgaat gcgggcgagt
    11221 tctgaacatc acgccactct tccggcgttt ctacgccctc aacaatcacc ccgcgacaat
    11281 agcgattcat tagatgtaaa agctgagaaa agagtgtgcg tccttccggc gactgacgca
    11341 gcatcacaaa cagttctcgc gcgattttga tgtagtcata acgcacttca cttagtgcag
    11401 agaaatttgc catcccggta ccaaaatcat ccagccacag cgggccaaat tcacacatcg
    11461 aggcaaaggt tgaatctttc ggcagacgga tatgctccac cagttcgaaa cgcagccagg
    11521 gaagacgctc aatctggcgc aggatttttg gttgctgacg cagggcgatg agcgtagggc
    11581 catcaatatt gaccgatgcc agcaggccgt gctctataaa gaagtcggct ttttgcgcca
    11641 gcaaatcaat ctgctctttc acaacctcca tccgatggct gacggtgatt tcagtaaaat
    11701 agcgatccgg cggcaggcgt tgcgaagggt tcaagggatg cgcgaccacc gttaatagct
    11761 ccacggccat taaccgcccg catgtttgat agatcggctg ccaggtgtaa gcacgctcac
    11821 actgcaacca aaaacgccgt tcctgcaagc tctcgatgct tgcttcaggg ttgcttattc
    11881 gctggataac ctgccttatc atcaaagatg tcctgtgttt aaaagtgatg ctgcccggac
    11941 tcgacaaaga ttatcggcgc gtaagggcag aactttaccg cctggccggg gtaaatttcc
    12001 tcactccaca acggaatctt tcaggaatac gtcctggctc aaaaaaataa tggaacactg
    12061 ttttaatatg gttgaccagc aaaccaccac agcgcaaact aacgctaatt ttttacagat
    12121 caggttcacg actatgtcca aaaagattgc cgtgattggc gaatgcatga ttgagctttc
    12181 cgagaaaggc gcggacgtta agcgcggttt cggcggcgat accctgaaca cttccgtcta
    12241 tatcgcccgt caggtcgatc ctgcggcatt aaccgtccat tacgtaacgg cgctgggaac
    12301 ggacagtttt agccagcaaa tgctggacgc ctggcacggc gagaacgttg atacttccct
    12361 gacccaacgg atggaaaacc gtctgccggg cctttactac attgaaaccg acagcaccgg
    12421 tgaacgtacg ttctactact ggcgtaacga agccgctgcc aaattctggc tggagagcga
    12481 gcagtctgcg gcgatttgcg aagagctggc gaatttcgat tatctctacc tgagcgggat
    12541 tagcctggcg atcttaagcc cgaccagccg cgaaaagctg ctttccctgc tgcgcgaatg
    12601 ccgcgccaac ggcggaaaag tgattttcga caataactat cgtccgcgcc tgtgggccag
    12661 caaagaagag acacagcagg tgtaccaaca aatgctggaa tgcacggata tcgccttcct
    12721 gacgctggac gacgaagacg cgctgtgggg tcaacagccg gtggaagacg tcattgcgcg
    12781 cacccataac gcgggcgtga aagaagtggt ggtgaaacgc ggggcggatt cttgcctggt
    12841 gtccattgct ggcgaagggt tagtggatgt tccggcggtg aaa
//
LOCUS       DAMZET010000119        12864 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.119, whole genome shotgun sequence.
ACCESSION   DAMZET010000119 DAMZET010000000
VERSION     DAMZET010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.119"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..153)
                     /locus_tag="POB34_003384"
     CDS             complement(<1..153)
                     /locus_tag="POB34_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl CoA-acyl carrier protein transacylase"
                     /protein_id="HDI5920271.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQG"
     gene            complement(169..1122)
                     /gene="fabH"
                     /locus_tag="POB34_003385"
     CDS             complement(169..1122)
                     /gene="fabH"
                     /locus_tag="POB34_003385"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HDI5920272.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(1190..2260)
                     /gene="plsX"
                     /locus_tag="POB34_003386"
     CDS             complement(1190..2260)
                     /gene="plsX"
                     /locus_tag="POB34_003386"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDI5920273.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(2341..2514)
                     /gene="rpmF"
                     /locus_tag="POB34_003387"
     CDS             complement(2341..2514)
                     /gene="rpmF"
                     /locus_tag="POB34_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDI5920274.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(2566..3087)
                     /gene="yceD"
                     /locus_tag="POB34_003388"
     CDS             complement(2566..3087)
                     /gene="yceD"
                     /locus_tag="POB34_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HDI5920275.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            3286..3870
                     /gene="yceF"
                     /locus_tag="POB34_003389"
     CDS             3286..3870
                     /gene="yceF"
                     /locus_tag="POB34_003389"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HDI5920276.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTL
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(3982..4941)
                     /gene="rluC"
                     /locus_tag="POB34_003390"
     CDS             complement(3982..4941)
                     /gene="rluC"
                     /locus_tag="POB34_003390"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HDI5920277.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            5514..8699
                     /gene="rne"
                     /locus_tag="POB34_003391"
     CDS             5514..8699
                     /gene="rne"
                     /locus_tag="POB34_003391"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HDI5920278.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQKVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(8896..9849)
                     /gene="flgL"
                     /locus_tag="POB34_003392"
     CDS             complement(8896..9849)
                     /gene="flgL"
                     /locus_tag="POB34_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDI5920279.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGFRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(9861..11504)
                     /gene="flgK"
                     /locus_tag="POB34_003393"
     CDS             complement(9861..11504)
                     /gene="flgK"
                     /locus_tag="POB34_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDI5920280.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNTGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(11570..12511)
                     /gene="flgJ"
                     /locus_tag="POB34_003394"
     CDS             complement(11570..12511)
                     /gene="flgJ"
                     /locus_tag="POB34_003394"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HDI5920281.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEEPTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(12511..>12864)
                     /gene="flgI"
                     /locus_tag="POB34_003395"
     CDS             complement(12511..>12864)
                     /gene="flgI"
                     /locus_tag="POB34_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HDI5920282.1"
                     /translation="QDAKVVINSRTGSVVMNREVTLDSCAVAQGNLSVTVNRQANVSQ
                     PDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRALNALGATPMDLMSILQ
                     SMQSAGCLRAKLEII"
BASE COUNT         3037 a         3523 c         3226 g         3078 t
ORIGIN      
        1 cccctgctgg gtcagcgccc acaggtcgta gcccagcgcc gcagaagctt cagcaaacgt
       61 ttcttcgaca attggatagc tcgccgccat atcagccagc attccaacgg tttgagaacc
      121 ctgtccaggg aacacaaatg caaattgcgt catgttttaa tccttatcct agaaacgaac
      181 cagcgcggag ccccaggtga atccaccgcc aaaggcttca agcagaacca actgccccgg
      241 cttaatgcgc ccgtcgcgta cagcttcatc cagcgcgcac gggacagagg ccgcagaggt
      301 attaccgtgg cgatccagcg tcaccacgac attgtccata gacataccga gttttttcgc
      361 cgttgcactg ataatacgca ggttagcctg atgcggaacc agccagtcca gttgagaacg
      421 gtcaagatta tttgccgcca gcgtctcatc aacgatgtgc gccagttccg ttaccgcaac
      481 cttgaagact tcgttgcccg ccatcgtcag atgaattgaa ttctctggat tcacacggtc
      541 agcattaggc agcgtcagca actcaccata gctaccgtcg gcatgcagat gggtggagat
      601 gattcccggc tcttcagagg cagccagcac cgcagcgccc gcgccatcgc caaaaataat
      661 aatagtccca cgatcggttg gatcgcaggt gcgcgccagt acatcggaac cgacgaccag
      721 agcatacttc accgccccag atttcacgta ttgatcggct acgcttaatg cataggtgaa
      781 acctgcgcag gctgctgcaa cgtcaaatgc cgggcaacct ttaatgccca gcatgctttg
      841 aatctgacaa gctgcgctcg ggaaagcgtg cgtagcagaa gtcgttgcca caacgatcag
      901 gccaatctgg tctttctcaa tgcccgccat ctcaattgcg cgtgtcgccg cttcaaagcc
      961 catggttgaa acggtttcgt ttggcgcggc aatgtggcgt tcgcggatac cggtacgagt
     1021 gacaatccac tcgtcagagg tgtccaccat tttttccaaa tcggcgtttg tccgcacttg
     1081 ttcgggcaga tagctgccag taccaataat cttcgtatac atgtacgctc agtcactttt
     1141 cggttatata ccgtcacttg caaactgcga gttcgctggc agcgtcctgc taccgcagag
     1201 ttccgctttt gccaccgtcc agcagctcaa aaccagctgg gtatacagat tccaggcgag
     1261 cggcaattcg ctgaggaact tgtcgctgca ccgcctgcac tgcctgttca atcgcgaccg
     1321 caaaagctcg ctgattggct gcaccatgac tttttatcac cgtgccgcgc aatcctaaca
     1381 gacaggcgcc gttatactgg tcggggttga ggtgactgaa tcgcctcgtc aggctctttt
     1441 gtagccaacg ctttaataac agtagccacc acgaccgttt tttcccttca ccctgagatt
     1501 tcagcagaga aaggaacatc ctgacaacac cctccatcgt ctttaatgtg acatttcctg
     1561 taaagccgtc acaaaccagc acatctgtct tgccagttaa caactcattg gcttcaagat
     1621 agccgatata attgatagaa gggattgttt taagcaccgc tgaggcatcc cgaatactgt
     1681 cgagaccctt tacttcttct tcaccaatat tgagcaacgc cacgcgagga ttgggaattt
     1741 ccaccacctc ttcagccaga actgagccca taatggcaaa ttgcaccaac attgtactgt
     1801 cacaatcgac gttggcccct aagtcaagga ccaccgtttt gcccttttgc tgatgtggta
     1861 ataccgtcac cagcgccgga cgctcaatcc cctccagggg cttgagtaat aattttgcca
     1921 gccccatcag tgccccggta ttaccggcac tgacacaggc ttgcgctcga ccttctttca
     1981 ccagctccag ggccacgcgc attgaactcc cacgactggc gcggatagct tgcgaaggcc
     2041 gggcatcact ggcgataact gactgcgcag gaataatctg cagacgcgaa cgttgttcaa
     2101 agtcagcttt agcaagtaat ggcgtgatgg cgtcgggatt gccgactaaa agaagagtga
     2161 gttgcgaatt agaattcagt gcctgcaatg ctgcaggcac tgtcacggaa gggccaaaat
     2221 cccctcccat gacatctaac gccagggtta gacgtgtcaa ggtatcgtcg ccgcccggtt
     2281 tggtctttcc ccagttgcct ggggaaaatc ctcactaagc ttcatcacgc agacgcgtga
     2341 ttacttagcg atgaccttgc ggccgcggta gtaaccgtcg gcagtgatgt ggtgacgcag
     2401 gtgtttttca ccagaagttt tgtctacaga caggctggtg actgcggtca gcgcgtcatg
     2461 ggaacgacgc atgccacgtt tggaacgggt tggtttattc tgttgtacgg ccatggacct
     2521 tactcctcaa ttacggttta tccccgatcc aacggggagc accaattact tacgctttaa
     2581 gctggctaat acggcaaatg ggtttggctt ttgcgcttct tcaggcagtt caccaaagac
     2641 catgtccgct tcggacactt cacagtgttc agaatcatgc accggaacta ccggcaaggc
     2701 gaggatgatt tcatcttcaa ccattgcaag cagatcgatt tcaccgaatt cgttaacctc
     2761 aatcggttca tacgcttccg gcagtgcttc agcctgttcg tctgaacgca caggactaaa
     2821 acaatacgtt gtgtagacct gatgagtaaa cggcttcccg caacgctgac actcgagcgt
     2881 taccgtcacc ttcgcatcgc cgtttaacac tgcgagacgt tggttatcga tagcgaacga
     2941 catggagcat tccacatcac tgtccacact gactacggat tcggcgacgc gctcaacctg
     3001 atcaggggta tagataccct ggtaatcaag gcgtttttga gccgtacgaa ccggatcgag
     3061 agtcaggggt aattttacct tttgcatagg gcgcgcatat taactttgta acgtcataga
     3121 gtcaaagaaa aaggcagcct gtggttgcct tttgccaata attcgcacac attgcgggtt
     3181 actgctttat tttcgttcag tgctgataac gaacatcaac ctgcaaaacg aagcgcatag
     3241 tttaaaatgg ctatcatcaa tacgctatat ctgatggaaa aaaatatgcc taaacttatt
     3301 ttagcctcca cctcgccatg gcgtcgcgct cttctggaaa aactgcaaat ctcttttgaa
     3361 tgtgcagcac cagaggttga cgaaacccca cgcagcgacg aatcaccgcg acagttggtg
     3421 cttcgactgg cacaagaaaa agcgcaatct ctggcgtcac gttatccgga tcatttaatt
     3481 attggttcag accaggtatg tgttcttgat ggcgaaatca ccggtaaacc gttaacggaa
     3541 gaaaatgccc gtctgcaatt acgcaaagcc agcggcaata tcgtcaccct ctatactgga
     3601 ctggcgttgt ttaattcggc gaatgggcat ctgcaaacag aagtggagcc ttttgacgtc
     3661 catttccgtc acctgagcga ggcggagatt gataattacg tgcgtaaaga gcatcccctg
     3721 cactgcgcgg gtagctttaa gagtgaagga tttggcatta cgctgtttga gcgtttagag
     3781 gggcgtgatc ctaacacgct ggttggttta ccgctaatcg cgctgtgtca gatgttacgt
     3841 cgggaaggga aaaacccgct gatgggatag ttttttgcgc cggatgtggt tacgtcgcat
     3901 ccggcaatcg gtgctggcgg cctgatgcgg cgtaaacgcc ttatcaggcc aaccgcccat
     3961 tatcaggttt atatgcttgt attagcgcgc gttacgcagc ttttgcaaac aacgcttcaa
     4021 accttcatcc atcggagctt cgatacgcat cacctcaccg gtccccggat gagtaaactt
     4081 caacgctgca gcgtgcagga acaggcgatt taatcccgtg cctgcttcag tgagctgcct
     4141 gtcaaattca cggtcaccgt agcgatcgtc aaaggcaatc ggatgacccg catattgtgt
     4201 atgcacacgg atctggtgag tacgccctgt taccgggcta caacgcacca gggtggcaaa
     4261 tgcatagcgt tcttccactt taaagcgtgt ttccgacggt ttgccttcct gactcacacg
     4321 cacgatacgt tcgccgcttt gcagaatatt tttcagtaac ggtgcttgaa cgctcttcac
     4381 atgcgactgc cactgaccgc gcaccagcgc caggtaatct ttttgcatcc ctttttcacg
     4441 taattgctca tgcagagaac gcaacgccga gcgttttttc gctaccagca aaacacctga
     4501 ggtgtcccgg tcaagacgat gaaccagttc aaggaaccgc gcttccgggc gcaacgcccg
     4561 caaaccttca ataacgccga agcttaaacc actgccgcca tgtaccgccg taccggaagg
     4621 tttattcagc accaggatgt gatcatcttc atataagatg acgtccgcca gcgccgccac
     4681 cttttgcaga tgtggcgaaa ccgcctcttc ttcccgctca gcaacgcgaa ccggtggaat
     4741 acgcacctca tcacccgctt cgagtttata ttcaggctta atacgttttt tgttcacccg
     4801 cacttcgcct ttacgcaaaa tacggtaaat catacttttt ggtacgcctt tcaattgggt
     4861 acgcaaaaag ttatcgatac gttgccccgc ttcgtcagcg gtgatagcaa caatttttac
     4921 ggatggagtc tctgttttca tggttggcga ttctaattag ccaacaggat tcgcgccact
     4981 catttttcta tgcttatatt tacttttgca ccttattact tcactgcgtg atcactttat
     5041 tgatggttat taaaccaatc accagcaaga agtgaaaaaa ctgtgagtaa gcgggtgata
     5101 aatggtaaaa gtcatcttgc tataacaagg cttgcagtgg aataatgagg ccgtttccgt
     5161 gtccatcctt gttaaaacaa gaaattttac ggaataaccc attttgcccg accgatcatc
     5221 cacgcagcaa tggcgtaaga cgtattgatc tttcaggcag ttagcgggct gcgggttgca
     5281 gtccttaccg gtagatggaa atatttctgg agagtaatac ccagtctgtt tctttgataa
     5341 ttgcgctgtt tttccgcatg aaaaacgggc aaccgacact ctgcgcctct ttgagctgac
     5401 gataaccgtg aggttggcga cgcgactaga cacgaggcca tcggttcaca cccggaaagg
     5461 cgttactttg cccgcagctt agtcgtcaat gtaagaataa tgagtaagtt acgatgaaaa
     5521 gaatgttaat caacgcaact cagcaggaag agttgcgcgt tgcccttgta gatgggcagc
     5581 gtctgtatga cctggatatc gaaagtccag ggcacgagca gaaaaaggca aacatctaca
     5641 aaggtaaaat cacccgcatt gaaccgagtc tggaagctgc ttttgttgat tacggcgctg
     5701 aacgtcacgg tttcctccca ctaaaagaaa ttgcccgcga atatttccct gctaactaca
     5761 gtgctcatgg tcgtcccaac attaaagatg tgttgcgtga aggtcaggaa gtcattgttc
     5821 agatcgataa agaagagcgc ggcaacaaag gcgcggcatt aaccaccttt atcagtctgg
     5881 cgggtagcta tctggttctg atgccgaaca acccgcgcgc gggtggcatt tctcgccgta
     5941 tcgaaggcga cgaccgtacc gaattaaaag aagcactggc gagccttgaa ctgccggaag
     6001 gcatgggact tatcgtgcgc accgctggcg tcggcaaatc tgctgaggcg ctgcaatggg
     6061 atttaagctt ccgtctgaaa cactgggaag ccatcaaaaa agccgctgaa agccgcccgg
     6121 ctccgttcct gattcatcag gagagcaacg taatcgttcg cgcattccgc gattacttac
     6181 gtcaggacat cggcgaaatc cttatcgata acccgaaagt gctcgaactg gcacgtcagc
     6241 atatcgctgc attaggtcgc ccggatttca gcagcaaaat caaactgtac accggcgaga
     6301 tcccgctgtt cagccactac cagatcgagt cgcagatcga atccgccttc cagcgtgaag
     6361 tccgtctgcc gtccggtggt tccattgtta tcgacagcac cgaagcgtta accgccatcg
     6421 acatcaactc cgcacgcgcg acccgcggcg gcgatatcga agaaactgcg tttaacacca
     6481 acctcgaagc tgccgatgag attgctcgtc agctgcgcct gcgtgacctc ggcggcctga
     6541 ttgttatcga cttcatcgac atgacgcctg tacgccacca gcgtgcggta gaaaaccgtc
     6601 tgcgtgaagc ggtgcgtcag gaccgtgcgc gtattcaaat cagccatatt tctcgctttg
     6661 gcctgctgga aatgtcccgt cagcgcctga gcccatcact gggtgaatcc agtcatcacg
     6721 tttgtccgcg ttgttctggt actggcaccg tgcgtgacaa cgaatcgctg tcgctctcta
     6781 ttctgcgtct gatcgaagaa gaagcgctga aagagaacac ccaggaagtt cacgccattg
     6841 ttcctgtgcc aatcgcttct tacctgctga atgaaaaacg ttctgcggta aatgccattg
     6901 aaactcgtca ggacggtgtg cgctgtgtaa ttgtgccaaa cgatcagatg gaaaccccgc
     6961 actaccacgt gctgcgcgtg cgtaaagggg aagaaacccc aaccttaagc tacatgctgc
     7021 cgaagctgca tgaagaagcg atggcgctgc cgtctgaaga agagttcgct gaacgtaagc
     7081 gtccggaaca acctgcgctg gcaacctttg ccatgccgga tgtgccgcca gcgccaacgc
     7141 cagctgaacc tgccgcgcct gttgtagccc cagcaccgaa agctgcaccg gcaacaccag
     7201 caactcctgc acaacctggg ctgttgagcc gcttcttcgg cgcactgaaa gcgctgttca
     7261 gcggtagtga agaaaccaaa ccgaccgagc aaccagcacc gaaagcagaa gcgaaaccgg
     7321 aacgtcaaca ggatcgtcgc aagcctcgtc agaacaaccg ccgtgaccgt aatgagcgcc
     7381 gcgacacccg tagtgaacgt actgaaggca gcgataatcg cgaagaaaac cgtcgtaatc
     7441 gtcgccaggc acagcagcag actgccgaga cgcgtgagag ccgtcagcaa gctgaggtaa
     7501 cggaaaaagc acgtaccacc gacgagcagc aagcgccgcg tcgtgaacgt agccgccgcc
     7561 gtaacgatga taaacgtcag gcgcaacaag aagcgaaggc gctgaatgtt gaagagcaat
     7621 ctgttcagga aaccgaacag gaagaacgtg tacgtccggt tcagccgcgt cgtaaacagc
     7681 gtcagctcaa tcagaaagtg cgttacgagc aaagcgtagc cgaagaagcg gtagtcgcac
     7741 cggtggttga agaaactgcc gctgccgaac caattgttca ggaagctcca gctccacgca
     7801 cagaactggt gaaagtcccg ctgccagtcg tagcgcaaac tgcaccagaa cagcaagaag
     7861 agaacaatgc tgataaccgt gacaacggtg gcatgccgcg tcgttctcgc cgctcgcctc
     7921 gtcacctgcg cgtgagtggt cagcgtcgtc gtcgctatcg tgacgagcgt tatccaaccc
     7981 agtcgccaat gccgttgacc gtagcgtgcg cgtctccgga actggcctct ggcaaagtct
     8041 ggatccgcta tccaattgta cgtccgcaag atgtacaggt tgaagagcag cgcgaacagg
     8101 aagaagtaca agtgcagccg atggtgactg aggtccctgt cgccgccgct gtcgaacctg
     8161 ttgttagcgc gccagttgtt gaagaaatgg ctgaagtcgt agaagccccc gtaccggttg
     8221 ccgaaccgca accggaagtg gttgaaacga cgcatcctga agtgattgcc gccgcggtaa
     8281 ctgaacagcc gcaggtgatt accgagtctg atgttgccgt agcccagaaa gtcgcagaac
     8341 acgcagaacc ggtggttgaa ccgcaggaag agacggcaga tattgaagaa gttgccgaaa
     8401 ctgctgaggt tgtggttgct gaacctgaag ttgttgctca acctgccgcg ccagtcgtcg
     8461 ctgaagtcgc agcagaagtt gaaacggtag ccgcggttga accagagatc accgttgagc
     8521 ataaccacgc taccgcgcca atgacgcgcg ctccggcacc ggaatatgtt ccggaggcac
     8581 cgcgtcacag tgactggcag cgccctactt ttgccttcga aggtaaaggt gccgcaggtg
     8641 gtcatacggc aacgcatcat gcctctgccg ctcctgcgcg tccgcaacct gttgagtaat
     8701 aattagctca aagtaatcaa gccctggtaa ctgccagggc ttttttattt catgtttgaa
     8761 tcaacatatt aaaattcatc aaatggcatc cttgctaacc aacaatgcaa aataggcaaa
     8821 aaaaatgccc gccccggcga tcccagagcg ggcagacaaa acatacccag tttcatgata
     8881 tgttttaaag cgagcttatt tgttgagctg gaagagcgac aatccctgca tatcggtaaa
     8941 tgctttatac gatgcctgca atgccgtttg ctgcatgatg taagatgaaa tagttgcatt
     9001 ccagtccaca tcaaccagat cgctcatctg ctgcgtttgc cctaaagcgc gatcgctacc
     9061 taaggaatcc agcgactcca gttcgttcag ctgcgtgcct aattccgcgc gaacggtaag
     9121 gacattattc agcgagtttt tcagtccacg gttggtttta tccaatgccg cagcggcgat
     9181 ttctttatcc gcttcgctat ccgcgaccgg cgttttcagg gctgcgatgg cgctatccag
     9241 catggcaaaa agattggttt cagaagcgct accgtctggt tccgctaccg cgttactggt
     9301 aatactgtcg aaaattttgt cgcccgtatg tcctatcacc atcgaacgcg aagcatcgac
     9361 ctgttgctta atactttctg ttccgccgac ataatcaccg ttagcctcac taaacggcgc
     9421 agtctctgtt ttataaccgg caaaaatgta gcgcccatta ccgtcagtgg tgttcgccag
     9481 attcagcaac tggtcacgaa acccctgaat atccgtagcc agcgaggccc ggtcatcgtc
     9541 actcaaggtg ccattgctgg cgtagacaat tttttcctga gcattctgga tagcagtggt
     9601 gacctggcta agtacactct cttccagtga cactttttga gtggcgaaag tacgtgccag
     9661 cgtgtactgg ctgttttgcg cctgtgcctg ggagagaaca acagcttgtg atgcagcaat
     9721 gggatcgtct gaagggttaa cgactcgctt acccgtcgac atctgctcgc cgtacttcat
     9781 ccattctgcc tgagaattgg tgatgccgcg catgttttgc tggtacatca tctgggtact
     9841 gaaacgcatt ttatctcccc ttagcgaatg ttaatcagcg catcaaaaat cgcgtttgcc
     9901 gtctgcagaa cctgcgcatt cgccaggtaa tactgctgaa aacgttgcag atttccgtac
     9961 tcctcatcga gattgacacc ggaaatcgac tgctgctgat tggaaagctg cgtcaccaca
    10021 ttaccttgcg tggcgctact ggttttcaac gtcgcggttt tattaccgat atcactcact
    10081 aacgaggcat aagcgtcgtt aaaggatttc gcaccgccca ccgttttact gttgctttgc
    10141 agatccagca gggcctgacc gttgcggtta tcgctatcac ccgcatcttc ttcgctcgcc
    10201 atcgctattt tcgcttcgtc ggtgattaat acatccatgt tgacgatagc gtcacttact
    10261 ggtttcagcg tgaagctgtc gttaacagca ggcgttcctg taaacgtcaa ctccagacca
    10321 tcaaatgcca ctttaccgtt ggcatccggc gtcaccgtaa aagtggtatt actggcaagg
    10381 cgggtgacct gccactgatt attatcgaac gagattttgt aatcagtcgc cagtaccgcg
    10441 gaggcatcag ttaccgtggc accgatcgca acgtcacctg tgtttttcgt gttttgcaga
    10501 accgcgggct taccgatagc aaagaaatct tcaccagcat cgccgttggc atcaaatccg
    10561 gctttgtgtt ggctgttgaa agcctcggca aatgccagcg ccagttgtcc aagcgtatta
    10621 cgcgtctggt ccagatcctg agaacggaat gtcagaatgc cgcccagcga cccggtattc
    10681 agtaatttct ccgggatctc aatattgcct gccgtcccat caacgtaagc gacagtcgta
    10741 cgagaagggt cggcgctgga aggaactgcc gccagttgcc gcgccgtact tccctgaacc
    10801 agtgagtaac cattggccat cgtgatgtta taagtaccgc catcctgaac gctgacttct
    10861 acgccaacaa tctggtttaa ttcgctcacc agttgatcgc gttgatccag cagattgtta
    10921 ggtgacgccc ctgcccccac gcctgtcagg cgcgagattt gatcgttcag gctggcaatt
    10981 tgtttagcgt aattgttgat ctgatcaacg ctggcaccta tcgcgatatt gacctgtttg
    11041 tcctggtcgc gcagatattg atcggtggtt ttaaactgat tcaccaaccc ttctgatttc
    11101 ccaatcagcg cctggcgcgc tgccgggtct tccgcgttac tcaccagcgt ttgcaggctg
    11161 gtgaagaaat cctgcatctg tgttgccagc gaagaggtac tggtggagag catattgtcg
    11221 attttcgaca tctgctcata gcgggcagtc aggccgctac tttgcgtctg cgccgcacgt
    11281 aactggttgg taataaaagc atcatactca cgctgcacac cagaaacgta gacaccattg
    11341 ccaacccagc cgccagcgcc caacgtgcta ttggcctgcg ccataatagt ggtttggcgg
    11401 gtatatccgg caacgttata gctggagata ttattacttg ccgtatttaa cgccgcctgg
    11461 gccgcgttca gtccgctcat ggcgttattg atcaagctgg acatggaggt tccttataag
    11521 ccttcaatta cagagtatta tcggcagcga cccgccggac ttgagttatt cagaacagat
    11581 tatcaatgtt catgctgtag gttttgctca ccttgtcgct tatcgatttc atctgctgga
    11641 tcatgttggt gagtttacgg gcatagtgag gatcggtagc ataccccgcg tcctgtaggg
    11701 cctgcgcccc ctgctccgca ctcgcggcgg tcgtcacggc ggcgtagcgc gggttacgtg
    11761 ttaacaaccc aacgtaatcc gacaatgctt ccagatacga gctgtagacc cgaaatttcg
    11821 cttttacttt cttcgcttcg ccattttcat attcagtcgt ggtgatttca gtgactggcc
    11881 ctttccagtt gccagaggct ttgacaccaa acaggttata gctcggctcg ccgttttcgc
    11941 ggcggatttg tcgttgcccc cagccagatt ccagcgccgc ctgagcgagg atcaaatgat
    12001 gtggcacacc gctttgctgg cttgccagtt gggcgggcag cgagagttgc gcgaggaatg
    12061 ctttactgtc acccggcagc gaatcatcgt agttacgtgg tacggccttt tgcaccagct
    12121 gagaaagcgc ctgattttga taacgcacca cagtttcgag cgggaatttc atcggtgctg
    12181 ctggcgtggg ctcctctggc aatggttgtt ctggcgtcat ctgtttaacc atcatctctg
    12241 caagccccag acctttgccc gccgtcatct gttgggcaat ctgctggtca tacatactgg
    12301 tatacaggcg agtgtgctcg ctgctgaaca ggccatcttt tggtaaagcg tcgcgcatgc
    12361 ttttcaacat catctgcacg aacatccctt ccacctggcg ggccaccgga cggatatttg
    12421 ccgccggatc ttcgcccgct ttcgccttta gttcgttgag tgattgcgca tcccaggccg
    12481 cacttgccag tagtttgctg tcgctgatca tcagatgatt tccagttttg cccgcagaca
    12541 tcccgcactt tgcattgatt gcaaaataga catcagatcc atcggcgtag cgcccagcgc
    12601 attgagcgcg cgcaccacgt tattgaggct ggcgctggaa cgtacgcttt gcagcgaacc
    12661 gccgctctgg cgtaagtcga tctgcgtttg tggcgttacc acggtctgtc cgccaccaaa
    12721 cggtgtatct ggctggctga cattggcctg acgattgact gtaactgaga gattcccctg
    12781 cgctaccgcg cagctgtcga gggtcacttc gcgattcatc accaccgaac cggtgcgcga
    12841 gttaatcact actttagcgt cctg
//
LOCUS       DAMZET010000120        12783 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.120, whole genome shotgun sequence.
ACCESSION   DAMZET010000120 DAMZET010000000
VERSION     DAMZET010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.120"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            166..1080
                     /gene="accD"
                     /locus_tag="POB34_003396"
     CDS             166..1080
                     /gene="accD"
                     /locus_tag="POB34_003396"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HDI5920283.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            1150..2418
                     /gene="folC"
                     /locus_tag="POB34_003397"
     CDS             1150..2418
                     /gene="folC"
                     /locus_tag="POB34_003397"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDI5920284.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            2408..3070
                     /gene="dedD"
                     /locus_tag="POB34_003398"
     CDS             2408..3070
                     /gene="dedD"
                     /locus_tag="POB34_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HDI5920285.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            3329..3817
                     /gene="cvpA"
                     /locus_tag="POB34_003399"
     CDS             3329..3817
                     /gene="cvpA"
                     /locus_tag="POB34_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HDI5920286.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            3854..5371
                     /gene="purF"
                     /locus_tag="POB34_003400"
     CDS             3854..5371
                     /gene="purF"
                     /locus_tag="POB34_003400"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDI5920287.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            5466..6035
                     /gene="ubiX"
                     /locus_tag="POB34_003401"
     CDS             5466..6035
                     /gene="ubiX"
                     /locus_tag="POB34_003401"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HDI5920288.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            6301..7083
                     /gene="argT"
                     /locus_tag="POB34_003402"
     CDS             6301..7083
                     /gene="argT"
                     /locus_tag="POB34_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HDI5920289.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            7304..8086
                     /gene="hisJ"
                     /locus_tag="POB34_003403"
     CDS             7304..8086
                     /gene="hisJ"
                     /locus_tag="POB34_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HDI5920290.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAEKYFDFDVYGG"
     gene            8176..8862
                     /gene="hisQ"
                     /locus_tag="POB34_003404"
     CDS             8176..8862
                     /gene="hisQ"
                     /locus_tag="POB34_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HDI5920291.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            8859..9575
                     /locus_tag="POB34_003405"
     CDS             8859..9575
                     /locus_tag="POB34_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5920292.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            9583..10356
                     /gene="hisP"
                     /locus_tag="POB34_003406"
     CDS             9583..10356
                     /gene="hisP"
                     /locus_tag="POB34_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HDI5920293.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVSGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            10553..11443
                     /gene="rpnB"
                     /locus_tag="POB34_003407"
     CDS             10553..11443
                     /gene="rpnB"
                     /locus_tag="POB34_003407"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HDI5920294.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(11491..12384)
                     /locus_tag="POB34_003408"
     CDS             complement(11491..12384)
                     /locus_tag="POB34_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDI5920295.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEPKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(12405..12767)
                     /gene="folX"
                     /locus_tag="POB34_003409"
     CDS             complement(12405..12767)
                     /gene="folX"
                     /locus_tag="POB34_003409"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HDI5920296.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
BASE COUNT         3019 a         3127 c         3469 g         3168 t
ORIGIN      
        1 cgcaaaataa aaagtaactc cgcggttcga ccactttttt atccaaagtt tcgggctgtt
       61 atgttttaat gtgcaacatt catggtctgt tgggggcaaa aatggcatta tgcgtcccca
      121 aagataaaac tggcatcgaa ccaggttcag acagaaaggt ccctaatgag ctggattgaa
      181 cgaattaaaa gcaacattac tcccacccgc aaggcgagca ttcctgaagg ggtgtggact
      241 aagtgtgata gctgcggtca ggttttatac cgcgctgagc tggaacgtaa tcttgaggtc
      301 tgtccgaagt gtgaccatca catgcgtatg acagcgcgta atcgcctgca tagcctgtta
      361 gatgaaggaa gccttgtgga gctgggtagc gagcttgagc cgaaagatgt gctgaagttt
      421 cgtgactcca agaagtataa agaccgtctg gcatctgcgc agaaagaaac tggcgaaaaa
      481 gatgcgctgg tggtgatgaa aggcactctg tatggaatgc cggttgtcgc tgcggcattc
      541 gagttcgcct ttatgggcgg ttcaatgggg tctgttgtgg gtgcacgttt cgtgcgtgcc
      601 gttgagcagg cgctggaaga taactgcccg ctgatctgct tctccgcctc tggtggcgca
      661 cgtatgcagg aagcactgat gtcgctgatg cagatggcga aaacctctgc ggcactggca
      721 aaaatgcagg agcgcggctt gccgtacatc tccgtgctga ccgacccgac gatgggcggt
      781 gtttctgcaa gtttcgccat gctgggcgat ctcaacatcg ctgaaccgaa agcgttaatc
      841 ggctttgccg gtccgcgtgt tatcgaacag accgttcgcg aaaaactgcc gccaggattc
      901 cagcgcagtg aattcctgat cgagaaaggc gcgatcgaca tgatcgtccg tcgtccggaa
      961 atgcgcctga aactggcgag cattctggcg aagttgatga atctgccagc gccgaatcct
     1021 gaagcgccgc gtgaaggcgt agtggtaccc ccggtaccgg atcaggaacc tgaggcctga
     1081 taactgataa gggcagggcc actggctctg cccttttgct attctcaccg taacgaatca
     1141 gcggatacca tgattatcaa acgcactcct caagccgcgt cgcctctggc ttcgtggctt
     1201 tcttatctgg aaaacctgca cagtaaaact atcgatctcg gccttgagcg cgtgagccag
     1261 gtcgcggcgc gtcttggcgt cctgaaacca gcgccatttg tgtttaccgt tgcgggtacg
     1321 aatggcaaag gcaccacctg ccgtacgctg gagtcgattc tgatggcggc agggtacaaa
     1381 gtgggcgtct acagttcacc gcatctggtg cgttataccg agcgcgtacg tgtgcagggg
     1441 caggaattgc cggaatcggc ccacaccgcc tcttttgcgg agattgaatc ggcacgcggt
     1501 gatatttccc tgacctattt cgagtacggt acgctgtcgg cgttgtggct gttcaaacag
     1561 gcacaacttg acgtagtgat tctggaagta gggctgggcg gtcgtctgga cgcaaccaat
     1621 attgtcgatg ccgatgtggc tgtagtaacc agcattgcgc tggatcatac cgactggctg
     1681 ggaccagatc gcgaaagtat tggtcgcgag aaagcaggca tctttcgcag cgcaaaaccg
     1741 gcaattgtcg gtgagccgga aatgccttct accattgctg atgtggcgca ggaaaaaggt
     1801 gcactgttac aacgtcgggg cgttgagtgg aactattccg tcaccgatca tgactgggcg
     1861 tttagcgatg ctcacggcac gctggaaaat ctgccgttgc cgcttgtccc gcaaccgaat
     1921 gccgcaacag cgctggcggc actgcgtgcc agcgggctgg aagtcagtga aaatgccatt
     1981 cgcgacggga ttgccagcgc aattttgccg ggacgtttcc agattgtgag cgagtcgcca
     2041 cgcgttattt ttgatgtcgc gcataatcca catgcggcgg aatatctcac cgggcgtatg
     2101 aaagcgctac cgaaaaacgg gcgcgtgctg gcggttatcg gtatgctaca tgataaagat
     2161 attgccggaa ctctggcctg gttgaaaagc gtggttgatg actggtattg tgcgccactg
     2221 gaagggccgc gcggtgccac ggcagaacaa ctgcttgagc atttgggtaa cggcaaatca
     2281 tttgatagcg ttgcgcaggc atgggatgcc gcaatggcgg acgctaaagc ggaagacacc
     2341 gtgctggtgt gtggttcttt ccacacggtc gcacatgtca tggaagtgat tgacgcgagg
     2401 agaagcggtg gcaagtaagt ttcagaatcg gttagtgggc acgatcgtgc tggtggcgct
     2461 gggggtgatt gtacttccag ggctgctgga cgggcagaaa aaacattatc aggatgagtt
     2521 cgcggctatc ccgctggtgc cgaaagcggg cgatcgtgat gagcctgata tgatgccagc
     2581 cgccacccag gcgttgccga cgcagccgcc ggaaggcgca gcggaagagg tgcgggcagg
     2641 tgatgccgca gcaccgtcgc tcgatccggc cactattgca gccaataaca ccgagtttga
     2701 accggaacct gcaccggtcg ccccaccgaa gccgaaaccg gtggagccgc ctaaaccgaa
     2761 ggttgaagca ccacctgcgc cgaagccaga accgaagccg gtcgtggaag aaaaagcagc
     2821 gccaacgggt aaagcctatg ttgtgcaact gggtgcgctg aaaaatgccg ataaagtgaa
     2881 tgagattgtc ggtaagctgc gcggtgccgg ttatcgggtt tatacgtcgc catccacgcc
     2941 agtgcagggt aaaattaccc gtattctggt tgggccggat gcctcgaaag ataagctgaa
     3001 aggttcgctg ggtgagttga agcaactttc tggcttaagt ggcgtggtaa tgggctatac
     3061 gccgaattaa tacagtcttg cctgatgcga cgctggcgcg tcttatcagg cctgggcagt
     3121 gttagaaccg taggtcggat aaggcgttta cgccgcatcc gacacgcatt gcacgatgcc
     3181 gcaaaggcat aaaaagtcga tggcgttgaa tattttttca gcgccatttt tattgatgcg
     3241 cgggaaggaa atccctacgc aaacgttttc tttttctgtt agaatgcgcc ccgaacagga
     3301 tgacagggcg taaaatcgtg ggacacatat ggtctggatt gattacgcca taatcgcggt
     3361 gattgctttt tcctctctgg ttagcctgat ccgcggcttt gttcgtgaag cgttatcgct
     3421 ggtgacatgg ggttgtgctt tctttgttgc cagtcattac tacacttacc tgtcagtctg
     3481 gtttacgggc tttgaagacg aactggttcg aaatgggatt gccatcgcgg tactatttat
     3541 cgctaccctg atcgttggtg ctatcgtgaa cttcgtgata ggccagctgg tggagaaaac
     3601 ggggttgtca ggcaccgatc gggtgctggg cgtctgtttc ggtgcgttgc gcggtgtgtt
     3661 gattgttgcc gccattctat tctttctcga ctcctttacc ggggtgtcga aaagcgaaga
     3721 ctggagcaaa tcacagctga tcccgcagtt cagttttatc atcagatggt tttttgatta
     3781 tctgcaaagc tcgtcaagtt tcttgcccag agcgtaagtg ctctgagatg tggcttaacg
     3841 aggaaaaaga cgtatgtgcg gtattgtcgg tatcgccggt gttatgccgg ttaaccagtc
     3901 gatttatgat gccttaacgg tgcttcagca tcgcggtcag gatgccgccg gcatcatcac
     3961 catagatgcc aataactgct tccgtttgcg taaagcgaac gggctggtga gcgatgtatt
     4021 tgaagctcgc catatgcagc gtttgcaggg caatatgggc attggtcatg tgcgttaccc
     4081 tacggctggc agctccagcg cctctgaagc gcagccgttt tacgttaact ccccgtatgg
     4141 cattacgctt gcccacaacg gcaatctgac caacgctcac gagttgcgta aaaaactgtt
     4201 tgaagaaaaa cgccgccaca tcaacaccac ttccgactcg gaaattctgc ttaatatctt
     4261 cgccagcgag ctggacaact tccgccacta cccgctggaa gccgacaata ttttcgctgc
     4321 cattgctgcc acaaaccgct taatccgcgg cgcatatgcc tgtgtggcga tgattatcgg
     4381 ccacggtatg gttgctttcc gcgatccaaa cgggattcgt ccgctggtac tgggaaaacg
     4441 tgatattgac gagaaccgta cagaatatat ggtcgcttcc gaaagcgtag cgctcgatac
     4501 gctgggcttt gatttcctgc gtgacgtcgc gccgggcgaa gcgatttaca tcactgaaga
     4561 agggcagttg tttacccgtc aatgtgctga caatccggtc agcaatccgt gcctgtttga
     4621 gtatgtatac tttgctcgcc cggactcgtt catcgacaaa atttccgttt acagcgcgcg
     4681 tgtgaatatg ggcacgaaac tgggcgagaa aattgcccgc gaatgggaag atctggatat
     4741 cgacgtggtg atcccgatcc cagaaacctc gtgtgatatc gcgctggaaa ttgctcgtat
     4801 tctgggcaaa ccgtaccgcc agggcttcgt taaaaaccgc tatgttggcc gcacctttat
     4861 catgccgggc cagcagctgc gtcgtaagtc cgtgcgccgt aaactgaatg ccaaccgcgc
     4921 cgagttccgc gataaaaacg tcctgctggt cgacgactcc atcgtccgtg gcaccacttc
     4981 tgagcagatt atcgagatgg cacgcgaagc cggagcgaag aaagtgtacc tcgcttctgc
     5041 ggcaccggaa attcgcttcc cgaacgttta tggtattgat atgccgagcg ccacggaact
     5101 gatcgctcac ggtcgcgaag ttgatgaaat tcgccagatc atcggtgctg acgggttgat
     5161 tttccaggat ctgaacgatc tgatcgacgc cgttcgcgct gaaaatccgg atatccagca
     5221 gtttgaatgc tcggtgttca acggcgtcta cgtcaccaaa gatgttgatc agggctacct
     5281 cgatttcctc gatacgttac gtaatgatga cgccaaagca gtgcaacgtc agaacgaagt
     5341 ggaaaatctc gaaatgcata acgaaggatg atgccttcgc tgagggtgcc ggtctggcac
     5401 cctgacttgc aactcccgcc gaaatcctgc aaagtctgcc tgcaagtctg acagggcaac
     5461 tatttatgaa acgactcatt gtaggcatca gcggtgccag cggcgcgatt tatggcgtgc
     5521 gcttattaca ggttctgcgc gatgtcacag atatcgaaac gcatctggtg atgagccagg
     5581 cagcgcgcca gaccttatcc ctcgaaacgg atttttctct gcgcgaagtg caggcattag
     5641 ccgatgtcac gcacgatgcg cgcgatattg ccgccagcat ctcttccggt tctttccaga
     5701 cgctagggat ggtgatttta ccctgttcaa tcaaaaccct ttccggcatt gtccatagct
     5761 atactgatgg cttactgacc cgtgcggcag atgtggtgct gaaagagcgt cgcccgttgg
     5821 tgctctgcgt gcgtgaaaca ccattgcact taggccatct gcgtttaatg actcaggcgg
     5881 cagaaatcgg tgcggtgatt atgcctcccg ttccggcgtt ttatcatcgc ccgcaatccc
     5941 ttgatgatgt gataaatcag acggttaatc gtgttcttga ccagtttgcg ataacccttc
     6001 ctgaagatct ctttgcccgc tggcagggcg cataactccg ctgttgccct gtttcagggc
     6061 aattttgcaa ccgcgatcaa atcctcgaca ttttgtttct gccattcaat cgaaacgctg
     6121 cgattcaacc gctatacctg ctatcttcaa cttcaggaca ataatgcaac gtcttattaa
     6181 catatttaac gttgaatgtt actgttgtcg tcaagatggc ataagacctg catgaaagag
     6241 cctgcaaaca cacaacacaa tacacaacat aaaaaagcca ttttcacttg agggttatgt
     6301 atgaagaagt cgattctcgc tctgtctttg ttagtcggtc tctccgcagc ggcttccagc
     6361 tatgcggcgc taccggagac ggtacgtatc ggaaccgata ccacctatgc gccgttctcg
     6421 tcgaaagatg ctaaaggtga ttttgttggc tttgatatcg atctcggtaa cgagatgtgc
     6481 aaacggatgc aggtgaaatg tacctgggtt gccagtgact ttgacgcgct gatcccctca
     6541 ctgaaagcga aaaaaatcga cgccattatt tcgtcgcttt ccattaccga taaacgtcag
     6601 caggagattg ccttctccga caagctgtac gccgcagatt ctcgtttgat tgctgccaaa
     6661 ggttcaccga ttcagccaac gctggattca ctgaaaggta aacatgttgg tgtgctgcag
     6721 ggatcaaccc aggaagctta cgctaacgag acctggcgta gcaaaggcgt ggatgtggtg
     6781 gcctatgcca accaggattt ggtctattcc gatctggctg caggacgtct ggatgctgcg
     6841 ttacaagatg aagttgctgc cagcgaagga ttcctcaagc aacctgctgg taaagatttc
     6901 gcctttgctg gctcatcagt aaaagacaaa aaatacttcg gtgatggcac cggtgtaggg
     6961 ctacgtaaag atgatgctga actgacggct gccttcaata aggcgcttgg cgagctgcgt
     7021 caggacggca cctacgacaa gatggcgaaa aagtatttcg actttaatgt ctacggtgac
     7081 tgatacgtcg ctgggaagct gtacctgatg gaatgatcat catggtgcac gccaggtttg
     7141 ttgcactatc gtggtgcatt gaaatgcata cttaagcatt tttaatgaaa aataatacgt
     7201 ctaacggggc gggatatttt gccttgatgg tcaattttat ggcacgataa gtgtaacaaa
     7261 cctgtaaata ttccctataa aaagactgtc agttgaggac attatgaaaa aactggtgct
     7321 atcgctctct ctggttctgg ccttctccag cgcaactgcg gcgtttgctg cgattccgca
     7381 aaacatccgc atcggtaccg acccgaccta tgcgccattt gaatcaaaaa attcacaagg
     7441 cgaactggtt ggcttcgata tcgatctggc aaaggaatta tgcaaacgca tcaatacgca
     7501 atgtacgttt gtcgaaaatc cgctggatgc gttaatcccg tccttaaaag cgaagaagat
     7561 tgacgccatc atgtcatcgc tttccattac ggaaaaacgt cagcaagaaa tagccttcac
     7621 cgacaaactg tacgctgccg attctcgttt ggtggtggcg aaaaattctg acattcagcc
     7681 gacagtcgag tcgctgaaag gcaaacgggt aggcgtattg cagggcacca cccaggagac
     7741 gttcggtaat gaacattggg caccaaaagg cattgaaatc gtctcgtatc aggggcagga
     7801 caacatttat tctgacctga ctgccggacg tattgatgcc gcgttccagg atgaggtcgc
     7861 tgccagcgaa ggtttcctca aacaacctgt cggtaaagat tacaaattcg gtggcccgtc
     7921 tgttaaagat gaaaaactgt ttggcgtagg gaccggcatg ggcctgcgta aagaagataa
     7981 cgaactgcgc gaagcactga acaaagcctt tgccgaaatg cgcgctgacg gtacttacga
     8041 gaaattagcg gaaaagtact tcgattttga tgtttatggt ggctaatcgc catcggtgaa
     8101 agaaagcccg ctcccttcgg gtaaccggag ggagacaaga ggcggtaatt caccacacac
     8161 gacaggacag gcagcatgtt gtatgggttt tcaggtgtta ttttacaggg tgcgctcgtc
     8221 acgctggagc tggctatcag ctctgtagtg ctcgctgtaa tcatcggttt aattggcgct
     8281 ggcggtaagc tctcgcaaaa tcggctttcg gggctgattt tcgaagggta caccacgctg
     8341 attcgtggcg tgccggactt agtgttgatg ctgctgattt tctacggttt gcagattgcg
     8401 ctaaacacgg tgacggaggc gatgggcgtc gggcagattg atatcgatcc gatggtcgct
     8461 ggtattatca ctctcggttt tatctacggt gcttatttta ccgaaacgtt ccgtggtgct
     8521 tttatggcag tgccgaaagg acatatagag gcggcgacgg cgttcggttt tactcgtggg
     8581 caagtgtttc ggcggatcat gtttccggcg atgatgcgtt acgccctgcc aggcattggc
     8641 aacaactggc aggtgatcct caaatctacc gcactggttt cgttactcgg cctggaagat
     8701 gtggtcaaag ccacccaact ggcaggcaaa agtacctggg aaccgttcta tttcgccatc
     8761 gtctgtggcg tgatttactt ggttttcacc actgtttcca atggtgtgct gctgttcctt
     8821 gagcgccgct actccgtggg tgtgaagagg gctgacctgt gatcgaaatc ttacatgaat
     8881 actggaaacc gctgctgtgg accgacggtt atcgctttac tggtgtggcg atcactctgt
     8941 ggctgcttat tttgtcggta gtgataggcg gagtcctggc gctgtttctg gcgattggtc
     9001 gtgtctccag taataaatac atccagtttc caatctggtt atttacctat atttttcgcg
     9061 gtacgccgct gtatgttcag ttgctggtgt tctattccgg catgtacacg cttgagattg
     9121 ttaagggaac cgaattcctt aacgctttct tccgcagtgg cctgaactgt accgtgctgg
     9181 cgctgacgct taacacctgc gcttacacta ccgagatttt tgctggggca atccgttcgg
     9241 ttccgcatgg ggaaattgaa gccgccagag cctatggctt ctcgaccttt aaaatgtatc
     9301 gctgcattat tttgccttct gcgctgcgta ttgcgttacc ggcatacagc aatgaagtga
     9361 tcctgatgct gcactctact gcgctggcgt ttacggccac ggtgccggat ctgctgaaaa
     9421 tagcccgcga tattaacgcc gccacgtatc aaccttttac cgccttcggc attgccgcgg
     9481 tgctctattt aatcatctct tatgtcctga tcagcctctt tcgcagagcg gaaaaacgct
     9541 ggttgcagca tgtgaaacct tcttcaacgc actgagaata cgatgtccga gaataaatta
     9601 aacgttatcg atttgcacaa acgctatggc gaacacgaag tgctgaaagg ggtgtcactg
     9661 caagcgaatg ccggagatgt aataagcatc atcggatcgt cgggatcggg gaaaagtacc
     9721 tttctgcgct gcattaactt cctcgaaaaa ccgagcgaag gatcgatcgt ggtcagtggc
     9781 cagacgatca atctggtgcg cgacaaagat ggtcaactca aagtcgccga taaaaatcaa
     9841 ctgcgcttac tgcgcacacg cctgacgatg gtattccagc acttcaatct ctggagccat
     9901 atgacggtgc tggaaaacgt catggaagcg ccgattcagg tgttgggcct gagcaagcag
     9961 gaagcgcgcg agcgggcggt gaagtatctg gcgaaagtcg ggatagacga acgtgcgcag
    10021 gggaaatatc cggtgcatct ttccggcggt cagcaacagc gtgtttctat cgcgcgggcg
    10081 ctggcgatgg aaccggaagt tttactgttt gatgaaccta cctcggcgct cgatcctgaa
    10141 ctggtaggcg aagtgttacg tattatgcag caactggcag aagaggggaa aaccatggtg
    10201 gtagtgactc acgaaatggg ctttgctcgc catgtttcta ctcacgtcat tttcctccat
    10261 caggggaaaa tagaagaaga gggcgcgccg gagcagttat ttggcaaccc gcaaagccct
    10321 cgtctgcaac agttccttaa gggatcgctg aaataatttc ctctccggtg gctaacgctg
    10381 ccggagtatt ttcatgggga atttgcgagt cagcttccgc tgaaatgtaa aagttgcagt
    10441 ccgctaaaaa tacttctgaa ttcgctgtcc aaagataaaa actgcctgct gcattgggtg
    10501 ttaatcgtgg ttattgtcgc gtcatcttaa atttgagggg tgacggaacg ccatgacaat
    10561 atcgacaact tccacgccgc atgatgcggt atttaaatct tttttacgcc atccagacac
    10621 cgcgcgggat tttattgata ttcatcttcc cgcgccgctg cgcaaactgt gtgatttaac
    10681 gacgcttaaa ctggaaccaa acagttttat tgatgacgac ctgcggcaat attattccga
    10741 cctcttgtgg tctgtgaaaa cgcaggaggg agtgggttat atttatgtag tgatagagca
    10801 ccaaagtaag ccggaagaat taatggcttt tcgcatgatg cgttattcca ttgcggcaat
    10861 gcaaaaccat cttgatgcgg gctataaaga gcttccattg gtgatcccga tgctgtttta
    10921 tcatggttgc agaagtcctt atccttattc actctgctgg cttgatgaat ttgccgagcc
    10981 tgctatagcc cgcaaaatat attcatcagc gtttccgttg gtggatatta ccgtggtgcc
    11041 ggatgacgag attatgcaac accgcaaaat ggcgctgttg gagttaattc agaaacatat
    11101 tcgtcagcgc gatctgttgg gattagtcga ccaaattgtt tcgctgctag ttacagggaa
    11161 cactaatgac agacagctaa aagccctgtt taattacgta ttacaaacag gggatgccca
    11221 gcgtttccgt gcatttattg gtgagataac agaacgcgca ccacaagaaa aggagaaact
    11281 gatgaccatt gctgacagat tacgtgaaga aggcgcaatg cagggcaaac acgaagaagc
    11341 cctgcgtatt gctcaggaga tgctggatag aggtttagac agagagttag ttatgatggt
    11401 gacccgacta tcgccagacg atcttatagc gcaaagccac taatcctgta acaccgggag
    11461 ttaactggcg gatgtttgct gtaaaccaca tcagcgaacg acatccgcca gcgcctcttc
    11521 taaatcgtac cagcgaaacg caaaacccgc ttcttccagc cgtttaggca gcgcgcgttg
    11581 tccacctaat accagtactg aagattcgcc cattaacagt cgaatggcgg tcgcggggac
    11641 gcgcaaaatg gccgggcgat gcagcgcatg accgagcgca tgggcaaatt gttcattgcg
    11701 taccgggtag ggcgaaacca tattaaatgg cccgcgcagc tcgttatcca gcagccagag
    11761 aataccgttg accatatcat caatatgaat ccaggccaga tactgccgac cagagccaat
    11821 cggcccgccc aggccaagac gaaacggcgg cagcatttta ccgagaatac cgccatccgg
    11881 tgccagtacc acgccggttc gcagcaggca cactcgcgtt ttgtcacttt gcgcccggca
    11941 ggcaatctct tcccagcggg cgcagagttt atgagtaaat tcgttatgcg gcggttcctc
    12001 ttcggtaacc accacttcac ctaagtcgcc ataatagccc gttgccgagc cggaaatgag
    12061 taccgacggc ggcgtgtcgc tggcattaat caaatcgacc agtttttgcg tgatattcca
    12121 gcggctttgg cagagacgct cttttggctc gtgagtccag cgtttatcag caatcggttc
    12181 tccggccagg ttgattaccg catcaacgcc gttgaggttg ctttgatcgg ctagcccttg
    12241 ccatagcgtc acccgagggc cgagaacgga actggctttc tgtgggttac gcgtcactac
    12301 cgtgatttga tggcccagct ccagcaaacg cggaatcaaa tggcgaccaa ttaatcccgt
    12361 ccctccggtg atcactatgt tcatgcagcc tcccgatatg gcggttaacg ctgccagctt
    12421 aaggtcatgg ataccgaatc ggcgtagcgc agcgcgtgca gtttatcgat ctccacttca
    12481 gcatacgtca cccagtgatg ttcacgtgcg atatcgagca catcctgagt taatttttcc
    12541 aacaaagaga aacggttatt ctctacatgc tgaataatgt ttttcgttac ggtgcgataa
    12601 ttcagcgcat cgttgatatc ttcgctagtg cgcgctttat cggcggggta gtggatcgtc
    12661 acattgataa caatatcctg acggttgtta atttcttctt ccttaattcc gataaacgta
    12721 cgtaaacgaa ggttctttat acgaataata gcggcaggtt gtgccattgc aggttctccg
    12781 ttg
//
LOCUS       DAMZET010000121        12494 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.121, whole genome shotgun sequence.
ACCESSION   DAMZET010000121 DAMZET010000000
VERSION     DAMZET010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.121"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1593)
                     /gene="flhA"
                     /locus_tag="POB34_003410"
     CDS             complement(<1..1593)
                     /gene="flhA"
                     /locus_tag="POB34_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HDI5920297.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVAD"
     gene            complement(1667..2677)
                     /gene="hypE"
                     /locus_tag="POB34_003411"
     CDS             complement(1667..2677)
                     /gene="hypE"
                     /locus_tag="POB34_003411"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HDI5920298.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAVDKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(2674..3795)
                     /gene="hypD"
                     /locus_tag="POB34_003412"
     CDS             complement(2674..3795)
                     /gene="hypD"
                     /locus_tag="POB34_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HDI5920299.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTNAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            complement(3795..4067)
                     /gene="hypC"
                     /locus_tag="POB34_003413"
     CDS             complement(3795..4067)
                     /gene="hypC"
                     /locus_tag="POB34_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HDI5920300.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(4058..4930)
                     /gene="hypB"
                     /locus_tag="POB34_003414"
     CDS             complement(4058..4930)
                     /gene="hypB"
                     /locus_tag="POB34_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HDI5920301.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIAEAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(4934..5284)
                     /gene="hypA"
                     /locus_tag="POB34_003415"
     CDS             complement(4934..5284)
                     /gene="hypA"
                     /locus_tag="POB34_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDI5920302.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            5496..5957
                     /gene="hycA"
                     /locus_tag="POB34_003416"
     CDS             5496..5957
                     /gene="hycA"
                     /locus_tag="POB34_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HDI5920303.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            6082..6693
                     /gene="hycB"
                     /locus_tag="POB34_003417"
     CDS             6082..6693
                     /gene="hycB"
                     /locus_tag="POB34_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HDI5920304.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            6690..8516
                     /gene="hycC"
                     /locus_tag="POB34_003418"
     CDS             6690..8516
                     /gene="hycC"
                     /locus_tag="POB34_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HDI5920305.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQALAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            8519..9442
                     /gene="hycD"
                     /locus_tag="POB34_003419"
     CDS             8519..9442
                     /gene="hycD"
                     /locus_tag="POB34_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HDI5920306.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFVIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            9460..11169
                     /gene="hycE"
                     /locus_tag="POB34_003420"
     CDS             9460..11169
                     /gene="hycE"
                     /locus_tag="POB34_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HDI5920307.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            11179..11721
                     /gene="hycF"
                     /locus_tag="POB34_003421"
     CDS             11179..11721
                     /gene="hycF"
                     /locus_tag="POB34_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HDI5920308.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            11721..12488
                     /gene="hycG"
                     /locus_tag="POB34_003422"
     CDS             11721..12488
                     /gene="hycG"
                     /locus_tag="POB34_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HDI5920309.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
BASE COUNT         2637 a         3395 c         3505 g         2957 t
ORIGIN      
        1 gtcggcgacc atttttttca gatcgcggtt agtcgcggcg attagacgca cgtccgtctg
       61 aatgattttg ttgctgccga gacgttcaaa ctcctgttcc tgcaatacac gcagcaactt
      121 cggctgtaac tccagtggca tatcgcccac ttcgtcgagg aacagggagc ttttatccgc
      181 cagttcaaaa cgaccaatgc gctgggcgct ggcaccggta aaagccccac gctcatgacc
      241 aaacagatcg ctttccagca atccggcagg catcgccgcg cagttcattt tgaccatgcg
      301 gcgattatta cgcccactga gattatggat cgcacgggca atcagctctt tacccgtgcc
      361 agtttcaccg aggatcagca cggtactgtc actttgcgcc accatttcaa cttgtttaag
      421 cacgctgtac atggcttcgc tgcggccaat aatctcgcca aattcactat caacattgtt
      481 gagctgctcg gtcagggcga ggttttcatc aaccagccgt tctttcagac gatggatttc
      541 ctgataggcg agggcgttat cgacagcgat tgccacacgt tcggcaatct ggcgcagtaa
      601 attcagattg gtagtggtaa acactttctc ttcgcattgc gccagtttca gcacgcccag
      661 catggtgtcg ccagacatca gcggtaacag gcacaaggtt tgaatctggt tgccccaggt
      721 gtcgaacaac atgcgttcat agggggctaa atcgtcccgc tcgtggagat tgatcagcag
      781 catctcttta cttttgaaca cgcgttcggt gagggttccg gcttcatcga cttcgctctg
      841 ttcgtgggcg ggatgttgtt tatcaagata gtgagtggag tagatgttga gtttgttttt
      901 acggtggctg cgtaagacaa tgctgatatc gtcaatgtcg aaatagtaat ggatttcttt
      961 ggcgacttcg ctgaccagtt cgtccatatc caggcgggaa agcaccgcgt tggtaatggc
     1021 aaccaggatg cggaagttat cgcgctcccg gcataacaac tcatagtcga cattgttgac
     1081 gacgcggctc tggatttgtt cggtgacgac agaaacgatc tgcgtaaatg tttgcagacg
     1141 attgaactct ttttcgctcc aggggcgatc gtcataacga ataaattcac agccaccaaa
     1201 aatatgccct tccgccgcca gtggcatcag gcaatagtga ccaaatttgg ggtatagccc
     1261 acctgcggcc agctgcggcc aggtttcaca aaattcttcg taactgcaat gcagcgtatc
     1321 agggcgcgac aaaatgctgc gtaccggacc gtgtgccaga acagtttcgt cttcatattt
     1381 aatgggggtg tctttttcac gcgatgcgta ataagacgca cgttgagtct gcgcttgcca
     1441 caacacaata gccgcgttgt cggcgagcgc agaacgcttt accagttgcg aaagagcctc
     1501 acacagcgag gccagatcgg gctgctgcaa tagtgtccga gtgatgtcga acaacccttg
     1561 ctgtccgaga tcactcatcg gtgtatatga cattttgttc ttccaggaaa gcaccgcaaa
     1621 gcggtgcaaa aggtcattga ctaactatag gagatttaga attttattag catatacgcg
     1681 gaagcggttc ggcgtgtggt aaatcgaggg ttcgtttcac gccatacaga ccggcaagac
     1741 gaacaccttt acgttccacc acttcaccaa tcagcgccgc gtctttcccc agtggatggg
     1801 aatgtaacgc tgccagcact tgctctgccg cgttgcgttc aacagctatt actagtttgc
     1861 cttcgttggc aaagttcagg gcgtccagtc ccagcaattc gcaaacgcca cgcacggcag
     1921 gtttaacagg cagtgccgct tctgaaagtt caataccaca accgcaggct gccgcgaact
     1981 catgaaccac cgcgtttaca ccaccacggg tggcatcacg cagcgctttc acgccgggaa
     2041 tgtcacgcag cgtctgaata agcggcgtca gcaccgcgca gtcgctgacc agttcgccat
     2101 ccagccccag ctgctcacgc aggttaagga tagtcgcccc gtggtcgccg agtgtaccgc
     2161 tcaccagcaa tacatcgcct gcggttagcg tctgtgcgcc ccagtgaata ttcgccggaa
     2221 ttgcgcccat gccagcggtg ttgataaaca gtttatctac cgcgccgcgc tgcaccactt
     2281 tagtatcgcc agtaacgatg gcaatgcctg ccgtgcgggc ggtttctgcc atgctggtca
     2341 ctacggcttt cagtgtctcc atcggcaatc cttcttcgag gataaagcca caggagagat
     2401 agcgcggaat agcgccactg accgcaacgt cattcgcggt gccgcaaatc gccagcttgc
     2461 cgatattacc gccaggaaag aacagcgggt caatgacgta actgtcggtg gagaacgcca
     2521 gacgatcgcc ttctgctacc agctgcgcca gatcaagacg tgcctggtct tcctgctctg
     2581 ccagccacgg gttggcaaag gcttccataa acaggctgtt gattaattgc tgcatcgcct
     2641 ggccgccgct gccgtgggcg agttggatat tattcacgct tcattctcct gctgacgata
     2701 ctgataccac gcggcgcacg ctccttcgga ggagaccatc agtgcaccaa atgcggtttg
     2761 aggattacag gtgttaccaa acagcgggca ttgatgcggc ttacatttgc ccgttaatac
     2821 ttcaccacaa cgtgcgcgcg ggtcatcgca gacctgctgc ggtgccgggc ggaaatgtgc
     2881 ttcggcatcg aatcgttgat agtccggcgt caggtgtaca ccagaagatt caatcacgcc
     2941 taagccgcgc cattcgctgt cgccgttgac gcagaagaca tccgcaatcg cctgttgcgc
     3001 cagcaggtta ccggcatccg gcactacccg ccgatactga ttttctacct tgctatgagc
     3061 agctattttc tgctccacca gcatgaccac gccttgtagt agatcaaggg gttcgaatcc
     3121 agcaaccacc agcggacgat gaaaatcgct ggcgataaaa ttataggcgt tggtgccgat
     3181 aaccatactg acgtgacctg gcgcgaggaa cgcatcgata ccgttatccg gctgttccag
     3241 caaactgcgc aacgtcggga taagcgtaat gtgctggcag aagaagtaaa aattctgcac
     3301 atcacgcgct ttcgcctgtt gcagagtgat ggcggtggtc ggcatggtgg tttcaaaacc
     3361 taagccgaag aacaccactt tgcgggttgg attctcctgc gccagtttca acgcatccat
     3421 tggcgagtaa acgatgcgaa catcggcacc gcgtgctttt gcctgcaaca gcgatccctg
     3481 tttccccggc acgcgcatgg cgtcgccaaa ggtacagaag atgacttccg gatggctggc
     3541 aatctccacg caggtgtcga ttctacccat cggcagtacg cacaccgggc aacccggacc
     3601 gtggataaac tcaacgtttt caggcagtaa ctggtcgagg ccgaatttaa aaatggcgtg
     3661 ggtatgacca ccgcacactt ccataatccg cagagggcgt tcggctgtgt aagagagatg
     3721 tgaagcacgt tcgcgcagat gctcaattaa ctgcatcacc tgttccggcg cgcgatattc
     3781 atcaacaaaa cgcattattt ttcctcgcca tacaacagcg cgccgacatc cggctcaacg
     3841 tcaaacatgt tttgtaaggc gtcgagagtg tcgcgtgctt cggcttcatt aattacgctc
     3901 atggcaaagc caacgtgtac cagtacccac tggcccacgc gcggctgacc gttttcatcg
     3961 cagctgccga ctaacgttaa atcgacatcg cgctgaatgc cgcagacgtc gactttcgcc
     4021 tggttgccgt caatggtgcg gatctggccg ggaacgccta tgcacatcgc tgtgtctcca
     4081 gccagttcag ccactggtcc atcccttcgc cgctggtggc ggaaataagg atgatttcaa
     4141 tttctggatt gacttcgcgg gcgcaggcga tgcacttctc aacgtcaaag ttgagatacg
     4201 gcagcaggtc aactttgttg agcagcatca gcgaggcggc ggcaaacata tgcggatatt
     4261 tcagcggttt gtcttcacct tcggtaacgg aaagcaccgc cactttgtgt ttttcaccga
     4321 gatcgaagct ggccgggcat acgaggttgc caacgttttc gataaacaga ataccgttat
     4381 cgtccagtgg caggcgcggt gcggcttcgg caatcatctg tgcgtcaaga tggcagcctt
     4441 taccggtatt cacctgaatc gctggtgtgc cggtagcgcg aatgcgtgcg gcatcgttca
     4501 cggtttgctg atcgccttca ataactgcgc acggaacgct gtctttcagg cgcattaggg
     4561 tttccgtcag cagggtggtt ttaccggaac cagggctgga aaccaggttg agcaccagtt
     4621 gcttgcgggc agcaaagcgc gcgcggttgc gttcagccag acggttattt ttgtccagca
     4681 cgtcaatttc gacttccagc atccgacgct ggctcatgcc cggcgcgtga gtgcctgctt
     4741 cgccatgacc gtaatgcagg tcgccttctt cagtctggct gggggtaaat tcaggcgctt
     4801 taatgccggt gattttcatc ttcgggcgtg ccgccggggc aaatggcgcg ctacgaaacg
     4861 cggaatgagg gttatgttca tcaccctcga tatacaggtt gccttcaccg caaccgcatg
     4921 ttgtacacat cgctcactcc tggtctattt ctatccgccg aatctgtaaa ccgtcgtctg
     4981 ccacaatctg tagcatgtca ccatgacact gtggacagcg gcggacgcgc tgggtcagta
     5041 gcgtcacata ctgttggcat gtttcacacc agcattcggc ctcttgttct tcgaggtgca
     5101 gtttacaacc ttccgccacg ctgccgcggc aaaccagatc aaaacaaaag gcaagagagc
     5161 tggtttcgac acaagaaaat gcgccaattt tgagccagac cccagttacg cgttttgcgc
     5221 cgtgttttgc ggcctgctgt tcgatcaatt ccagtgcccg ttggcagagg gttatttcgt
     5281 gcatatcgcc tcccattaac tatggccagc tacaagcaat aattgtgcca gtattgatta
     5341 tccctgcggt gaataatgtc gatgatgtcg aaatgacacg tcgacacggc gacgaaattc
     5401 atctttagct taaaaatcgt tttaataaca atgaattaaa agttggcaca gaaaatgctt
     5461 aaagctggca tctctgttaa acgggtaacc tgacaatgac tatttgggaa ataagcgaga
     5521 aagccgatta catcgcacag cggcatcgtc gcctacagga ccagtggcac atctactgca
     5581 attcgctggt tcaggggatc acgttatcga aagcgcgcct gcatcacgcc atgagctgcg
     5641 cgccggacaa agaactctgt ttcgtccttt ttgaacattt tcgcatttac gtcaccctgg
     5701 cggatggctt taacagccac accatcgagt attacgtcga aacaaaagat ggcgaagaca
     5761 aacagcggat tgcgcaggcg caactgagca ttgacggcat gattgatggc aaggtcaaca
     5821 tccgcgatcg cgaacaggtt ctggaacact atctcgaaaa aatcgctggc gtttacgaca
     5881 gcttatacac cgctattgaa aacaatgtgc cggtgaattt aagccaactg gtaaagggac
     5941 aaagcccggc agcatgagct gaggctttgc ccgttttgca ggcgttacgc ctgtttgggg
     6001 atgggcgtgt cgatgagtgt cgaaaatgac atttcatcgg catgttttcg tcaaaaatga
     6061 caatcacctg aggaatgcct ggtgaatcgt tttgtaattg ctgactccac gctctgtatc
     6121 ggctgccaca cttgtgaggc cgcctgttca gagacgcatc gccagcacgg cctgcaatca
     6181 atgccgcgcc tgagagtgat gctgaatgaa aaagaatctg cgccgcagct ctgtcaccac
     6241 tgtgaagatg caccctgcgc ggtggtctgc ccggttaacg ccatcacccg cgttgatggg
     6301 gccgtgcagt tgaatgaaag cctgtgcgta agctgcaagc tgtgcggcat cgcctgcccg
     6361 tttggcgcaa ttgaattttc cggcagccgt ccgctggata ttccggcaaa cgccaatacc
     6421 ccgaaagcgc caccggcacc gcctgctccg gcgcgtgtca gcacattgct tgactgggtg
     6481 ccaggtattc gcgcgatcgc cgtcaaatgt gacctttgta gctttgatga acaaggtccg
     6541 gcctgcgtgc ggatgtgccc gactaaagcc ctgcatctgg tggataacac tgatatcgcc
     6601 cgcgtcagca aacgtaagcg tgagctgact tttaacacgg actttggcga tctcaccttg
     6661 tttcagcagg ctcaaagtgg agaggctaaa tgagcgcaat ttccctgatc aatagcggcg
     6721 tggcgtggtt tgtcgccgcc gctgttctgg catttctctt ttcttttcaa aaagcgttaa
     6781 gtggctggat agctggaatt ggcggcgcgg ttggtagtct gtatacggca gccgcgggct
     6841 tcactgtact gactggcgcg gttggcgtga gcggtgcgct gtcgctggta agctacgatg
     6901 tgcaaatctc tccgcttaac gcgatttggc tgattacgct cggtctgtgc ggtctgtttg
     6961 tcagcctcta caacattgac tggcatcgcc acgcgcaggt gaagtgcaac ggcttgcaga
     7021 tcaatatgtt gatggctgcc gccgtctgcg ccgtcattgc cagcaacctc ggcatgttcg
     7081 tggtaatggc cgaaatcatg gccctgtgcg cggtgttcct caccagcaac agcaaagagg
     7141 gcaaactgtg gtttgcgctg gggcgtcttg gcactctgct gctggcgatt gcttgctggc
     7201 tgctgtggca gcgttatggc acgctggatc tgcgcctgct ggatatgcgt atgcaacagc
     7261 tgccgctcgg ttccgatatc tggctgctcg gtgtgattgg ctttggcctg ctggccggga
     7321 ttattccgct gcacggctgg gtgccgcagg cacatgctaa cgcctctgcg ccagctgccg
     7381 cgttgttttc tacggtagtc atgaaaattg gcctgctggg cattttaacc ctgtcactgc
     7441 tgggcggtaa tgcaccgctg tggtggggga tcgcgctgct ggtgctcggc atgatcaccg
     7501 cgttcgtcgg tggtctgtat gcgttgatgg aacacaacat ccagcgcctg ctggcttacc
     7561 acaccctgga aaatatcggc attatcctgc tggggctggg cgctggcgta acgggtatcg
     7621 cgctcgaaca accggcactg attgctcttg gcctggtcgg tggtctgtac catctgctta
     7681 accatagcct gttcaaaagc gtactgttcc tcggagcggg gagcgtctgg ttccgtaccg
     7741 gtcatcgcga tatcgaaaaa ctcggtggta ttggcaagaa aatgccggtt atctccatcg
     7801 ccatgttagt cgggctgatg gcaatggctg cgctgccgcc gctgaatggt tttgccgggg
     7861 aatgggttat ctatcaatcc ttcttcaaac tgagcaatag tggcgcgttt gttgcccgtc
     7921 tgctggggcc gctgctcgcc gtgggtctgg caattaccgg tgcgctggcg gtgatgtgta
     7981 tggcgaaagt ctatggcgtc acgttcctcg gcgcgccgcg taccaaagaa gccgaaaacg
     8041 ccacctgtgc accgctcctg atgagcgtaa gcgtagtggc actggcgatt tgctgcgtaa
     8101 ttggcggtgt tgctgcgccg tggctgctgc cgatgctgtc tgctgctgta cctctgccgc
     8161 tggagcctgc taacaccacc gtttctcaac cgatgatcac gttgctgctg attgcctgcc
     8221 cgctgctgcc attcatcatt atggcgattt gcaaaggcga tcgtttgcca tcgcgttccc
     8281 gcggcgcggc ctgggtgtgc ggctacgacc acgaaaaatc aatggtgatt accgctcacg
     8341 gttttgccat gccggtgaaa caggcgcttg cgccagtgct gaaactgcgc aaatggctga
     8401 atccggtgtc gctggtgccg ggctggcagt gcgaaggtag tgcgttgctg ttccgccgga
     8461 tggcgctggt tgaactggcg gtactggtgg tgattattgt ttcacgagga gcctgagaat
     8521 gagtgtttta tatccgttaa ttcaggcgct ggtgttattt gccgttgcgc cgctgctctc
     8581 cggtattacc cgcgtggcgc gcgcccgtct gcataaccgt cgtgggccgg gtgtgttgca
     8641 ggagtatcgt gacattttca aactgctggg ccgccagagc gttggcccgg atgcctccgg
     8701 ctgggtgttc cgcctgacgc cgtatgtgat ggtgggcgtc atgctgacta tcgctactgc
     8761 gctgccggtg gtgaccgtcg gttctccgct gccgcaactg ggtgatttaa tcaccttact
     8821 gtatctcttt gtcatcgcgc gtttcttctt tgccatttct ggtctggata ccggtagccc
     8881 gtttaccgct atcggcgcga gccgtgaagc gatgcttggc gtgctggtcg aaccgatgct
     8941 gctgcttggt ctgtgggttg ccgcacaggt tgccggttcc accaacatca gcaacatcac
     9001 cgacaccgtt tatcactggc cgctgagcca gagcatcccg ctggtgctgg cgctttgtgc
     9061 ctgtgcgttc gccactttta tcgaaatggg caaactgccg ttcgacctgg cggaagccga
     9121 gcaggagttg caggaaggtc cgctctccga atacagcggt agcggctttg gcgtcatgaa
     9181 atggggtatc agcctgaaac agctggtggt gttgcagatg ttcgtcgggg tgtttattcc
     9241 gtggggacaa atggaaacct tcaccgccgg tggactgctg ctggcgctgg tgattgccat
     9301 cgtcaaactg gtggtcggcg tcctggttat cgcgctgttc gaaaacagca tggcccgtct
     9361 gcgtcttgat attactccgc gcattacctg ggctgggttt ggctttgcat ttttagcgtt
     9421 cgtctccttg ctggcggcgt gattaaagag agtttgagca tgtctgaaga aaaattaggt
     9481 caacattatc tcgccgcgct gaatgaggca tttccgggcg tcgtgctgga ccacgcctgg
     9541 cagaccaaag atcagctgac tgtcaccgta aaggtgaact acctgccgga agtggtggag
     9601 tttctttact acaaacaggg tggctggctg tcggtgctgt ttggtaacga cgaacgcaaa
     9661 ctgaatggtc attacgccgt ttattacgtg ctgtcgatgg agaagggcac taagtgttgg
     9721 gtaacggttc gcgtcgaagt tgacgccaac aaaccggagt atccgtccgt gacgccgcgc
     9781 gttccggcgg ctgtgtgggg cgagcgcgaa gtgcgcgata tgtacggttt gattccggtt
     9841 ggtctgccgg atgaacgtcg tctggtgctg ccggatgact ggccggatga actttatccg
     9901 ctgcgtaaag acagcatgga ttatcgtcag cgtccggcgc cgaccaccga tgctgaaacc
     9961 tacgagttca tcaatgaact gggtgacaag aaaaacaacg tcgtgccgat tggtccgttg
    10021 cacgtcactt ccgacgaacc gggtcacttc cgtctgttcg tcgatggcga aaatattatc
    10081 gacgccgact accgcctgtt ctacgtccat cgcggcatgg aaaaactggc ggaaacccgt
    10141 atggggtaca acgaagtgac cttcctctct gaccgtgtgt gcggcatctg cggctttgct
    10201 cacagcaccg cctacaccac gtcggtggaa aacgcgatgg gtattcaggt gccagaacgt
    10261 gcgcagatga tccgcgccat tctgctggag gtggaacgcc tgcactcgca tctgctcaac
    10321 ctcggcctgg cctgtcactt tactggcttc gactccggct ttatgcagtt cttccgcgtg
    10381 cgtgaaacct ccatgaaaat ggcagagatc cttaccggtg cgcgtaaaac ctacggcctg
    10441 aacctgatcg gcgggattcg tcgcgatctg ctgaaagatg acatgatcca gacccgccag
    10501 ctggcgcaac aaatgcgtcg tgaagtgcag gagctggtgg atgtgctgct gagtacgccg
    10561 aacatggaac agcgcactgt cggcattggt cgtctggacc cggaaatcgc ccgcgatttc
    10621 agtaacgttg gcccgatggt ccgcgccagc ggacacgctc gcgatacccg cgccgatcac
    10681 ccgtttgttg gttatggcct gctgccaatg gaagtccaca gcgagcaggg ctgcgacgtt
    10741 atttcccgtc tgaaagtgcg tatcaacgaa gtctataccg cgctgaacat gatcgactac
    10801 ggtctggata acctgccggg tggtccgctg atggtggaag gctttaccta cattccgcac
    10861 cgtttcgcgc tgggctttgc cgaagcgccg cgcggcgacg atatccactg gagcatgacc
    10921 ggcgacaacc agaagctgta ccgctggcgc tgccgtgccg cgacctacgc gaactggccg
    10981 accctgcgct acatgctgcg tggcaatacc gtttccgatg cgccgctgat tatcggtagc
    11041 ctggaccctt gctactcctg taccgaccgc atgaccgtgg tcgatgtgcg taagaagaag
    11101 agcaaagtgg tgccgtacaa agaactcgag cgttacagca ttgagcgtaa aaactcgccg
    11161 ctgaaataag gaatcgccat gtttaccttt atcaaaaaag tcatcaaaac cggcacggcg
    11221 acctcgtctt atccgctgga gccgattgcg gttgataaaa acttccgtgg taagccagag
    11281 cagaacccgc agcagtgcat cggctgcgcg gcctgcgtca atgcctgccc gtcaaacgcc
    11341 ttaacggttg aaactgacct cgccacagga gagcttgcgt gggagtttaa tctcgggcgc
    11401 tgcatcttct gtggacgctg cgaagaagtc tgcccgacgg cagcgatcaa actgtcgcaa
    11461 gagtacgaac tggcggtgtg gaagaaagaa gatttcctgc aacagtcccg ttttgtgctg
    11521 tgcagctgtc gcgtctgcaa tcgtcctttc gccgtccaga aagagatcga ctacgccatt
    11581 gcgctgctta agcacaatgg tgacagccgc gcggaaaacc accgcgaaag ctttgagact
    11641 tgcccggaat gtaagcgcca gaaatgcctg gtgccgtccg accgtattga actgactcgc
    11701 catatgaaag aggccatctg atgagcaatt tattaggccc ccgtgacgcc aacggcattc
    11761 cggtcccgat gacggtggat gaatccatcg ccagcatgaa ggcgtcgtta ctgaaaaaaa
    11821 tcaaacgttc tgcctacgtt taccgcgtgg actgcggcgg ctgcaacggc tgcgaaatcg
    11881 aaattttcgc cacgctttcg ccgctgtttg atgcagaacg cttcggcatt aaagtcgttc
    11941 cttcaccgcg tcatgcggat attttactgt ttaccggcgc ggtcacccgt gcaatgcgat
    12001 cccctgcgct gcgtgcgtgg cagtccgcgc cggacccgaa aatctgtatc tcctacggtg
    12061 cctgcggtaa cagtggcggg atcttccacg atctctactg cgtgtggggc ggtacggata
    12121 aaatcgtccc ggtagatgtc tacatccctg gttgcccgcc aacgcctgcc gccacgctgt
    12181 acggctttgc aatggcgctc ggcctgctgg agcagaaaat tcacgcccgt ggcccgggtg
    12241 agcaggatga acaaccggcg gaaatcctgc atggcgatat ggtgcagccg ctgcgtgtga
    12301 aagtggatcg cgaagcacgc cgcctggcag gttatcgtta cggtcgtcag attgccgatg
    12361 acttcctgac acagttaggg cagggcgaag aacaggttgc acgctggctg gaagcggaaa
    12421 acgatccgcg tctgaacgag attgtcagcc atctgaatca tgttgtagaa gaggcgcgta
    12481 tccgatgagt gaaa
//
LOCUS       DAMZET010000122        12401 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.122, whole genome shotgun sequence.
ACCESSION   DAMZET010000122 DAMZET010000000
VERSION     DAMZET010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.122"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(251..595)
                     /locus_tag="POB34_003423"
                     /pseudo
     CDS             complement(251..595)
                     /locus_tag="POB34_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(630..749)
                     /locus_tag="POB34_003424"
     CDS             complement(630..749)
                     /locus_tag="POB34_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDI5920310.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(710..895)
                     /locus_tag="POB34_003425"
     CDS             complement(710..895)
                     /locus_tag="POB34_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920311.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            996..1169
                     /locus_tag="POB34_003426"
     CDS             996..1169
                     /locus_tag="POB34_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920312.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            1171..1515
                     /locus_tag="POB34_003427"
     CDS             1171..1515
                     /locus_tag="POB34_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HDI5920313.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            1519..1854
                     /locus_tag="POB34_003428"
     CDS             1519..1854
                     /locus_tag="POB34_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HDI5920314.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(1911..4232)
                     /locus_tag="POB34_003429"
     CDS             complement(1911..4232)
                     /locus_tag="POB34_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDI5920315.1"
                     /translation="MQGKNTIVITGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDVN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGAELLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(4349..4558)
                     /locus_tag="POB34_003430"
     CDS             complement(4349..4558)
                     /locus_tag="POB34_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920316.1"
                     /translation="MKLKKLSGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
     gene            complement(4958..5176)
                     /locus_tag="POB34_003431"
     CDS             complement(4958..5176)
                     /locus_tag="POB34_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920317.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVNHRDQQ"
     gene            complement(5308..6831)
                     /locus_tag="POB34_003432"
     CDS             complement(5308..6831)
                     /locus_tag="POB34_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDI5920318.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVAVE"
     gene            complement(7163..7411)
                     /locus_tag="POB34_003433"
     CDS             complement(7163..7411)
                     /locus_tag="POB34_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920319.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMNNTTLTDIIINDRESVLS"
     gene            complement(7524..7790)
                     /gene="ariR"
                     /locus_tag="POB34_003434"
     CDS             complement(7524..7790)
                     /gene="ariR"
                     /locus_tag="POB34_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HDI5920320.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            complement(7819..8091)
                     /locus_tag="POB34_003435"
     CDS             complement(7819..8091)
                     /locus_tag="POB34_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HDI5920321.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            complement(8134..8370)
                     /locus_tag="POB34_003436"
     CDS             complement(8134..8370)
                     /locus_tag="POB34_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HDI5920322.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            8684..9895
                     /gene="bluF"
                     /locus_tag="POB34_003437"
     CDS             8684..9895
                     /gene="bluF"
                     /locus_tag="POB34_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HDI5920323.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            10100..10831
                     /gene="bluR"
                     /locus_tag="POB34_003438"
     CDS             10100..10831
                     /gene="bluR"
                     /locus_tag="POB34_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HDI5920324.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            11052..11456
                     /locus_tag="POB34_003439"
     CDS             11052..11456
                     /locus_tag="POB34_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HDI5920325.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            11509..11619
                     /locus_tag="POB34_003440"
     CDS             11509..11619
                     /locus_tag="POB34_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920326.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHHANTIKLIFEM"
     gene            11855..11899
                     /locus_tag="POB34_003441"
     CDS             11855..11899
                     /locus_tag="POB34_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920327.1"
                     /translation="MFSKLAQSSIKAMF"
     gene            12152..>12401
                     /gene="iraM"
                     /locus_tag="POB34_003442"
     CDS             12152..>12401
                     /gene="iraM"
                     /locus_tag="POB34_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HDI5920328.1"
                     /translation="MKWIVIDTIIQPSCGISFSAIWGNMKMIIWYQSTIFLPPGSIFT
                     PVKSGIILKDKEYPITIYNIAPFNKDLWSLLKSSQECPP"
BASE COUNT         3621 a         2712 c         2497 g         3571 t
ORIGIN      
        1 tccacctccc cctttacacc ttaaggctgt tcccacgcac agaacaaaat gtttatgcct
       61 ggttgagtaa ataaccctgt tgttagtgtg gatatactgg gagatgataa ccccgcaatt
      121 tcattattca ttgttctccc ataggatgag gtttcccgcc gtaactggcg ggcttttttt
      181 gccccagaat tttctctttc aacatcctgt aaacgaaaac tgcgccgaag cgcagtttaa
      241 tcgttgatgg tcagaacgac cagttcacac cagccaccgc gttccacggg gactccacac
      301 cggcaccatg gctatacccc accccgaaat gcccgcttaa cgtactgctg aatgaggctt
      361 taatacctgc ctggtatatt ccacgtctgc ccgacaaatc attgacaaaa ttaccgtcac
      421 tattcacttt cacccgatta tcatcgacaa attctttgcg cacagccgcc ttcaggcagg
      481 gttcaatttc cataccgttc cccagacgca tgttgtaact cagcgttgca cccagttcac
      541 gatatatact gcgggtatcg actgatttcg attccatgcc attggataaa tgatagtagc
      601 gtctaagtga cctaagctct gctgcaacat cagcccgcag gcaccagacc aggggattca
      661 tcctgaagag acagcgcagg tgtattgtgt tcaccgctca ccaaagacat catgatgaaa
      721 tgatgatatc ccgcataaga aagaggcatt ttttacacgc agtgcgctga agtgtagttg
      781 gataaaaaat cactcttttc agataatact gtcgtacgct tatctttcga caatgaggcc
      841 gtcggcaaaa taaaatgact aatgtaatcg ttgctgatga atatcttctg ctcacataaa
      901 aatcacacaa taatattgat aaatcagatt atcttacctt tacaacattg cgttaacagt
      961 caatgacact tcttgaataa aaatattgtc aatcaatgag ttattcctca tttaagatta
     1021 ttctcattca cgtaaaaaac ataattccta tcatcacagc cacgctcatt ttgaattatc
     1081 tgaataatag cgagcgttct ttagttaagc agatcttaat tgaagacgaa attattgtgt
     1141 gtgcaactta cttaataccg gacatctaag ttgaagaaaa aaatacttgc cttcgggctt
     1201 atctcagcat tgttctgctc cacgccagcg atggcggata tgaatcgcac cacgaaaggt
     1261 gcattgctag gcgcaggcgt tggtttactt actggcaatg gtgttaatgg cgtactgaaa
     1321 ggtgcagctg ttggcgctgg tgttggtgca gtaacagaaa aaggccgcga cggtaaaaat
     1381 gcgcgtaaag gtgcaaaagt aggtgccgcc gttggtgctg tcactggcgt tctgaccggc
     1441 aatggcctcg aaggggcgat taaaggtgcc gtgattggcg gtacgggtgg tgcaattctg
     1501 ggtaaaatga aataaggaat gatgatgaaa aaatttgctt tactggcggg cctgtttgtt
     1561 ttcgcaccaa tgacctgggc gcaggactac aatattaaaa acggtttgcc gtcagaaacc
     1621 tatattactt gcgcagaagc caacgaaatg gcaaaaactg acagcgcaca ggttgcagaa
     1681 attgttgcgg taatgggtaa tgccagcgtt gccagccgtg atttaaaaat tgagcaatct
     1741 ccggaactga gcgccaaagt ggtagaaaaa ctgaatcagg tttgcgctaa agacccgcaa
     1801 atgctgttga tcacggcaat tgatgacacc atgcgtgcta tcggcaaaaa ataatatttc
     1861 atatgtaccg cgtcgatgaa tacgacgcgg ttttttagct aacggcggga tcagaactta
     1921 tatttcaggc ccaccatcac tgcggtgtca ttgtagccat tatcacccag ctgcacgccc
     1981 acattccccc agacgctggc cgctggattc aggttgccat ttacgccaag tttaatttcc
     2041 cccaaattac tcgccccatc ttgttcgact ttcacaccgt tcattgagac agcaaaatct
     2101 ttactgttat ggatccagtt catttcaaca aaaggttcaa agtcgtcacc gtgcggaccg
     2161 ctgtttttct cctgagtacg aataaaggtg cggaaaccaa gtcgggtctg gatgttgtta
     2221 tttgcgtcat tctcaatacg tgagccattg gcttcggtgt gcgcattatg atcgacgccc
     2281 atccagatag cctgtgcctg tggctggaat atccacgtgt atttcgccgc ttccagacca
     2341 aaggatttat tcagggcaaa gccgtaaccc gcttccagcg agacggttgc gcctttcgca
     2401 ctataggatt cataggctaa ttcatcccct ttcaccgagt ttttaaacca gctatattgc
     2461 gcccaggcgt caatgtatgc gcctttttta ctgatgtcat cggcaaacca agtggcatac
     2521 agccctgcgc aatagcctct gacactccct ttcgaacgat aatccgacac gctggaatga
     2581 gttgagttgt agtcgcgggc ataaccagcc atcactccca aacgccaact gtcactgtcg
     2641 gtaaaaccac cggttaacag ctcggcccct aactgcgaga cgtagcgatg cgaggtcgtt
     2701 ctcaactgtc cgttgctgtc acgccaggca ttatgcccgc caatttgacg tagccaaagt
     2761 cggcttgaac gctcctgttc agttacagga tcgatgtagc gcatttcacc cgcacggtcg
     2821 ttcagacgca taacaaaaag agtgttagcg gctgccaggt tggcgacgta acttccagct
     2881 tcgaggcgca ggacgggggt aggcgcaaca gtaggcgctg aacttcctcc ggagtttgtt
     2941 tcaccgccgc tcgttgcctg gaagttagtc agataccagt ttttattggt cgcttcggta
     3001 ttacctcggg ttaatgaata ttcgtaggca ccggcgaaaa tcctcgaatc cttaataaat
     3061 tccccatttg attccccctc aacgctgata atctctatcc catttaacgt gtaagctccg
     3121 ctgcctcctg cattggtaac tttaactcgg gttgttccag aggtatttcc attaataacg
     3181 actttatccg tagctgaatt atcatcaccc aattcagttc tgagatgcaa tacgccatta
     3241 ttaccaacgt aattttcagt tattgttaat cttgttggtt cgacatcccg tccatctgcc
     3301 cgggaaatat agaccgtact attatcaacc gtgaggttgc tcaccgttga gtcagcttta
     3361 atatcccagg tactattatc tgaaagcgac agataggtat gagtgttaac atctgttgaa
     3421 atattagctg aacccgttat ttttgaatta gttgcatcga cggtcaaatg acctttaata
     3481 ttattgataa aaacatctgc agccgatgat acctcactat ccgtaatggt cacgttactc
     3541 gtctcatttt tatggtagag gatttcattt gctgaccgta gtgagctacc agataaaata
     3601 tctaccgttg cttcctgacc atttatggaa gactccagca caattccaga cccctgatgc
     3661 gcgactgcag atgtattttt cagcgtcacc gtgccgccat tataaatttt agcagtcgcg
     3721 gcgtcaggac ccgttgtata aatttctccc tggttaaatg aaactgtccc ctggcgggca
     3781 gcgatggcat gagcattgtc cccatgcgtg gtgatatcaa catcggttaa tgtaacgctg
     3841 gcattggtat aatcaagcgt gtcaccagcc ttgacgacag tgccattagc gtacgcggca
     3901 taactttctg ttccgtgggt tgtgatctcc ccttttgcca tatccagttt tgctatggca
     3961 ttattttcat tacttcctgt atcggtagta ctttcttcat caagagaaga tacacacgca
     4021 cgcgcgcttc cgggagatgc acacgcaaga acgccgactg cgtgattacc cgcagtaaca
     4081 ataaatgtat catctccgtc agaaaaagag ggggtaaccg aaccgtcaga gttgactctt
     4141 attatggtgt cagtattcag gtttccactc gtttgactta gcaaaccaat tgagtaatcc
     4201 cccgttatga caatcgtgtt tttaccctgc aaaataacag caccgccaaa ttcacttacg
     4261 attgcgctag ctgttaatgc tgtgcttaga ggactattgt cagaaggata taaaccgccc
     4321 gttgttacaa tgacgccatt gttaatggtt aacttgctac cctctcctgc aacggatacc
     4381 cgtaacttcc attacccgtt gttataacta gcgcattatc aatatctttt acttttccat
     4441 cttttattgc cacagcatgt ttatattcat cgtgctgaat aaactgttca gcatatgcat
     4501 tacccaccgc cagagcaata agtcccaaac taaacccgga gagttttttc agtttcatag
     4561 attaaccatc cattcattgg attaacaaac attcctggtc aaataaccta tgcgttcaga
     4621 ttaccaaaac ggagggtaaa gacaaaaaca aaagcacaaa aaatttaaat agataaacag
     4681 cgacaatata taaatgataa ataagtcggc attatcattt ttcaccttat tttcatgaca
     4741 ttgatcactt tagggtgacg attttgttta acaaataact cttcgacatc aaaattgtta
     4801 atagttgcga tgaggattat ttcggtgata tataaaaaca accgtgataa caccagggtt
     4861 gacaactatt tgcgcaataa tgcctttcct tcaatgatcc aggcgttcac tgccagcaac
     4921 ccattcagcc gcatactgac agtgagaaac tgaaagatta ttgctgatcc ctgtggttga
     4981 cgagtctttc tcttaatcga ttaaccagat tgatcgccag ccaaacaagg gcattaagca
     5041 aatttatcgt cacaagcact tctcccagcc cggtactggg ttttgcaata aggatgacca
     5101 ggagggtgat cagcataaat gcaatggaaa agatgatata gagagtgaaa taatccagat
     5161 tattactatt gttcatgcta ctaatggcaa tcctgtcaaa actttcaaac aattatagca
     5221 aacaaaataa tgaacaatag taacaagagg caacgagcta attagctgtc tataattaca
     5281 ccgcactatg gccttactga tcaattttca ctcaaccgca accttcacct tcggtttaga
     5341 aaggataatt ttgaccaggt cgatgtatgt aacaggttta tagaaataat aaccctgctg
     5401 aaatgtgata ttattttggt tcagatagtc aagttgttct ttcgtttcga caccttcagc
     5461 gacgatactg atcgaaagtt tacgcgccag atccaataca caatccagaa ttcgggttga
     5521 ttctgggtct gcgtttacgc ggccaacgaa actgtgatca attttgatat aatcaatatg
     5581 caggtcatga agataagaaa gccctgagta gccagtaccg aaatcatcca gcgcgatgac
     5641 aaaaccattt tcatgcagaa tgttcaaccg ctgaaccaga ctttcatcaa cattcaatgg
     5701 ctcacgctcg gtgacttcca gaacaaggtt taaatcgcgg cgggtaaaac tgtcacggaa
     5761 atttaaacac tcgtcgacaa acgtcggcga aataatatgc gaagcgctaa aattaattcc
     5821 aatatgaaaa ccttccggca atttactcgc gatagcgttc atctgtctgg caacctgatt
     5881 catcaggctt tgcgtaagcg gaacgattaa tccggatttt tcagcaagtg gaataaatgc
     5941 cgcgggtgat atatatccac cgtgaggttg tttccagcgg gctaacacct caactccccg
     6001 caatgtccct tcccgaccat ttaccacagg ttggtaaaaa gggacgatct ccccattatt
     6061 tattgcccgt cgcaggatct cttcaggcgt tgcgctttta ttgaaatatc tatcaagcag
     6121 gaaggcagcg gcgcaggcga ttaacagaat aaatattaaa atcccaaagc ctttatcgat
     6181 aagtctgtag aagctaaaga gtggcggcgg gttaaagata atagtgaaat gatattttgt
     6241 ggagtgaacc ttctcggtat agggataatg tccattaagc gtcatcacat cacccgttgg
     6301 tccaataatg tcatccgcca cgcgtaatac ataccttacc cctttcaagg gtacattaag
     6361 tgccccacga atatgctgat cgcttatcgt aaccaaaatg cgcgtatctg caaattggct
     6421 ctgatagagc aggataggta atctattaac ggtgtcgatt gccggagcca acagtaaatt
     6481 actgtccggg acaacaggaa tacgagacag caggacccga ttcccaggca gggatgtaca
     6541 ccagactatt ccctgtttga gaatgatgat tgtgcgcagg tgaggtttca gagctgcttc
     6601 agtgccaagc tgatattgct cctcgaggtc gcattccttc ccggcaatgt taaccgctgt
     6661 ttgagtcgcc tgtgacgctt cttcaagtat atgattgata ttgttggcgg catatcttgc
     6721 tcccgccagg tactctgctt ttgcggaata ccagagctgg atgttcaaaa tagcgacacc
     6781 ggtaataaac aagcatatcg caacgaggat gggtatgagt gtattgcgca tttgcttgtc
     6841 ccggccaaag ggtttaatac atgaaaatgc cattcattcg ctcgcgctgg catctacaga
     6901 gagcatggta gagagcataa gaaatatcca cgccgaagat aaaattttct ccatcttgat
     6961 ctaacccatt aattattgag cataatgtag gcagtacaaa ataagttagg cgggatatca
     7021 ggcgtcaaga atggagagcg agaactctcc attcttgaca cctgatattg cggacataat
     7081 aagaaagcat aacgcctgaa atgctcactt tgtatcagca tggtgataca gctgatgttt
     7141 attctaaaac cttactcaag ttctaagaga gcacggattc cctgtcatta ataatgatat
     7201 ccgttagtgt agtattattc atttcaatgt actgcaatat ttgcagatag agatcagaaa
     7261 gaatcacact ggtttcatct tttaaacgca agcgtaaagc ctgatcaata atatgactat
     7321 caacataccc atgcgtcatg aacacctccc tctcgagatt tttaaacaaa gcacattgaa
     7381 aaataaatct ctctgggatt gaattattca tgtcagcctc gtagaaatat taacctaata
     7441 aaattatgct gtgtatagtc taaagcgaca gacaaaaata agatgaatga gttttctata
     7501 aacttatact taataattag aagttacata tcatcagctg tgtatcgcaa cacgatttcc
     7561 agtgtttttc taatcacatc agctttgaga atatcacttg ttgtttccag ggagtgtatc
     7621 agacttaaga taatattttt attattaaca ttatcgcctg aaagcattaa attggtgaca
     7681 gcctgcccta acactgctga ctcttcttct aacaaattac ccgaactgcg aaagtaactt
     7741 gctaacgctt tatcagttat tgcattatga attgtagtat cttcaagcat ggtaacctct
     7801 catcttactt atgaaatttt aatgtattct gtttattttc ttaccattga acatactttt
     7861 attgtcagct gtcgctgttc ttcggtttgg ccctcctgaa tcactattgc taaaatcacg
     7921 aattatgctg tcaattaaat cgcgtcggaa agcatcttcc tcagccgatt tcattacgag
     7981 taattgctta ataagagtct gcaatgaaaa atttatcttc atgcgcagta tctggagcac
     8041 cgcctggcca gtaatttcat attttatacg ttcattatca gatgtcttca taacgacttc
     8101 atagctaacc tgttaaataa tcaggctgtt gcattattca aaaagcaacc caattagtgc
     8161 attataatgt ttctgttcct cttcccctgt cgcttgttcc agtcggcaaa gaagtttggc
     8221 acaaagcgac tttcgactca gattacgtcc atctttcaaa atctcagtca caatttcccc
     8281 gagagtttcc tgctgagtag gcaagttagc ttttgcaaag taacgagtga ttgctcccgc
     8341 agaatctgaa gtcactgaat tttgatgcat gctacgcctc tgttaaaaat gttaatcaat
     8401 gtgaagtgag gtaacaatag caaacttgta cgaaatatgt gtacagaatg ataaattaat
     8461 tttttgcaat tagtgctaat gcatataaaa caatgtgtta tgataaaaca tattgcatca
     8521 tttaactgac aatctataca agatttttcg ccaactagtg ttcaactttt tccatttttt
     8581 atcaatgtaa agaaacggtt atgttttggt attaaaagaa agacacaaga agatatgtct
     8641 gttaccgtct tactctcgcc tcacccatta ccctggattg ttaatgctta ccacccttat
     8701 ttatcgtagc catatacgtg acgacgaacc tgtcaaaaaa atcgaagaaa tggtttcgat
     8761 agcaaatcgc aggaacatgc agtctgacgt aacagggatc ttactgttta atggttctca
     8821 ttttttccag cttctggaag gtccggaaga acaggttaaa atgatatatc gggctatatg
     8881 ccaggatcca cggcactata atattgttga gctgctgtgc gattacgcgc ctgctcgccg
     8941 ttttggcaaa gcgggaatgg aattatttga tttgcgcctg cacgagcgag atgacgtttt
     9001 acaggccgta ttcgacaaag gcacatcaaa atttcagcta acttatgatg acagagcgct
     9061 acaatttttt cgtacttttg tccttgcaac cgaacaatca acctatttcg agatccctgc
     9121 cgaagactcc tggcttttta tcgctgacgg atctgataaa gaacttgatt cctgtgccct
     9181 ttcaccaact ataaacgacc actttgcctt tcatcctatt gtcgatccct tatcgcggcg
     9241 gataatcgct tttgaagcca ttgtgcaaaa aaatgaagat agcccatcag ccatagcggt
     9301 tgggcagcgt aaagacgggg aaatctacac agcggatctc aaaagtaagg cgcttgcatt
     9361 cacgatggca cacgcacttg agctcggtga taaaatgatt tcaatcaatc tattacctat
     9421 gaccctggtt aacgaacctg acgcagtctc ttttttactt aatgaaataa aggccaatgc
     9481 tctggtgcct gaacaaatca tcgttgaatt tactgaaagt gaagtcatat ctcggtttga
     9541 tgagtttgcc gaagcgatta aatcgctaaa ggctgctggt atcagtgtag caattgatca
     9601 ttttggcgca ggttttgctg gtttgttact cctgtcacgc ttccagcctg acagaattaa
     9661 aatcagtcag gaattgatta ccaatgttca taaaagtggg ccacggcagg caattattca
     9721 ggcgatcata aaatgctgta catcacttga aattcaagtc agtgctatgg gcgtggcaac
     9781 accagaagag tggatgtggc ttgaatccgc aggaattgag atgtttcagg gagatctgtt
     9841 tgctaaagct aaattgaatg gtatcccttc catagcgtgg ccagagaaaa aataattttc
     9901 agcacattct ttcacatgat ttcagtaaat catcgtgaaa atgaatacgt tacgtttaaa
     9961 tttaatccac attaaaaata atgtttcatc caatttagct gaaatcttat ttatactttg
    10021 tacaatttta gcgtatattg tacaaagtat tattgggtcg tgtacaggcg acggagattt
    10081 gtgaccgcaa ggaggaattg tggcttatta cagcattggt gatgttgctg aacgttgcgg
    10141 gattaatcct gtcactctcc gggcctggca acgccgctac ggtttgttaa aaccacagcg
    10201 cagtgaaggc ggacaccgac tctttgatga agaagacata caacgcatcg aagagatcaa
    10261 gcgttggata agtaatggcg tccctgtagg caaagttaaa gcattactgg aaaccaccag
    10321 ccaggatacg gaagatgact ggagccgcct gcaagaagag atgatgtcaa ttcttcgcat
    10381 ggctaatcct gccaaactac gcgcgagaat tatttcactg ggtcgagagt acccagtaga
    10441 tcaattgatt aatcatgttt accttcctgt tcgccagcgt ctcgtgcttg atcacaacac
    10501 ctcccgcatt atgagcagta tgtttgacgg cgcattaatt gaatacgcag caacctcgct
    10561 tttcgaaatg cgccgtaagc ccggtaaaga agccattctg atggcgtgga atgttgaaga
    10621 gagagcacga ttgtggctgg aagcatggcg tttatcattg tcaggatggc acatttctgt
    10681 tcttgctgat cccattgaat cgccgcgccc ggaactgttc ccgacgcaaa cattgattgt
    10741 ctggacaggc atggcaccaa cgagaaggca aaacgaactt ttacaacatt ggggtgagca
    10801 aggttataaa gtcatctttc atgcccccta atttcacctt tatcccgccc cttagacaga
    10861 atgttataat ctgaacccac aacagcgttt gtaccggagc tggtccgttg caaaatgctc
    10921 accgggagaa ttcagatatt ccctctatca cactctgtac atatcacagc caaaggacag
    10981 accagattga agtggtaaaa ccgatcgtgc acactacgca gtctcacaac taacaagagg
    11041 ttattatcct gatggatcag gtcgttgttt ttcaaaagat gtttgagcaa gtgagaaaag
    11101 agcaaaactt ctcctggttt tattcagaat taaaacatca ccgtattgca cattacattt
    11161 attatctggc tacggataac atcagaatta ttactcacga tgacacggtt ttgttattaa
    11221 gaggaaccag gaacctgtta aaagttagta cgaccaagaa ccctgctaaa ataaaagagg
    11281 ccgcattgct tcatatttgc ggaaaatcta catttcggga atactgttca acactagcag
    11341 gcgcgggcgt tttccggtgg gttactgatg ttaatcataa caaacgcagt tactacgcca
    11401 ttgataatac gcttttatat attgaagatg tagaaaataa taaaccatta atctagctaa
    11461 agttggatgc ttaagaaatg cttcataatt cagtaaggca ttagcataat gggaataaaa
    11521 gtacagagac tatccctatg gatgataaat actgtccttt tattgctacc cataaataat
    11581 caccatgcca atactatcaa attgatattt gaaatgtgat cacttgactt tctatacgtt
    11641 attttataac ggttaatata tttataaaaa caacgggtgt gccatacgcc cgtttcaata
    11701 cttaacgcac atgtgttttg gtttagtcat catccagtta tatgtatttt agccaggaac
    11761 aggttaaacc tttcctatat aactcaaaaa ttgaaacctt attctcatgt catgcttata
    11821 ttcattatta tcgttatata aaaaggcaac cataatgttt agcaaattgg cacaaagtag
    11881 cataaaggct atgttttaat tacaggatgt tcagtcattt gaatgtataa cattatagct
    11941 aaacaaatct aaaacgaagt gaataattta ttgttttctc taaatctcat tttgtttaac
    12001 atccattgag attccttgct ttaaattttg ttttatataa gcaatcgttt taattaattt
    12061 attttttgaa ggggtaatat actcatatgc aaaataaaga aatgaacatc caaatgaact
    12121 atattaaata ccgtgggata agacataaca aatgaagtgg atagtaattg acacgataat
    12181 tcaaccttca tgcggcatat ctttttcagc catatggggt aatatgaaaa tgatcatctg
    12241 gtatcaatct actatatttc tccctcctgg cagtatattt acaccggtta aatctggaat
    12301 tatccttaaa gataaagaat atcctattac tatttataac atcgcaccat tcaacaagga
    12361 tttatggagt ctactcaaaa gcagtcaaga gtgtcctcca g
//
LOCUS       DAMZET010000123        12285 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.123, whole genome shotgun sequence.
ACCESSION   DAMZET010000123 DAMZET010000000
VERSION     DAMZET010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.123"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..570
                     /gene="fliH"
                     /locus_tag="POB34_003443"
     CDS             <1..570
                     /gene="fliH"
                     /locus_tag="POB34_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDI5920329.1"
                     /translation="LEQQLAQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLARGLEQG
                     LAEAKSQQAPIHARMQQLVSKFQTTLDALDSVIASRLMQIALEAARQVIGQTPTVDNS
                     ALIKQIQQLLQQEPLFSGKPQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGC
                     KVSADEGDLDASVATRWQELCRLAAPGVV"
     gene            570..1943
                     /gene="fliI"
                     /locus_tag="POB34_003444"
     CDS             570..1943
                     /gene="fliI"
                     /locus_tag="POB34_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HDI5920330.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGTETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            1962..2405
                     /gene="fliJ"
                     /locus_tag="POB34_003445"
     CDS             1962..2405
                     /gene="fliJ"
                     /locus_tag="POB34_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HDI5920331.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            2402..3529
                     /gene="fliK"
                     /locus_tag="POB34_003446"
     CDS             2402..3529
                     /gene="fliK"
                     /locus_tag="POB34_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HDI5920332.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVAGNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQHSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            3634..4098
                     /gene="fliL"
                     /locus_tag="POB34_003447"
     CDS             3634..4098
                     /gene="fliL"
                     /locus_tag="POB34_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HDI5920333.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            4103..5107
                     /gene="fliM"
                     /locus_tag="POB34_003448"
     CDS             4103..5107
                     /gene="fliM"
                     /locus_tag="POB34_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HDI5920334.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            5104..5517
                     /gene="fliN"
                     /locus_tag="POB34_003449"
     CDS             5104..5517
                     /gene="fliN"
                     /locus_tag="POB34_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HDI5920335.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            5520..5885
                     /gene="fliO"
                     /locus_tag="POB34_003450"
     CDS             5520..5885
                     /gene="fliO"
                     /locus_tag="POB34_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HDI5920336.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQPDFQSVMKNLLKRSGRS"
     gene            5885..6622
                     /gene="fliP"
                     /locus_tag="POB34_003451"
     CDS             5885..6622
                     /gene="fliP"
                     /locus_tag="POB34_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDI5920337.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            6632..6901
                     /gene="fliQ"
                     /locus_tag="POB34_003452"
     CDS             6632..6901
                     /gene="fliQ"
                     /locus_tag="POB34_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HDI5920338.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVSTLFTNLPYIIG"
     gene            6909..7694
                     /gene="fliR"
                     /locus_tag="POB34_003453"
     CDS             6909..7694
                     /gene="fliR"
                     /locus_tag="POB34_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HDI5920339.1"
                     /translation="MLQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLTLLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            7984..8607
                     /gene="rcsA"
                     /locus_tag="POB34_003454"
     CDS             7984..8607
                     /gene="rcsA"
                     /locus_tag="POB34_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HDI5920340.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(8651..8839)
                     /gene="dsrB"
                     /locus_tag="POB34_003455"
     CDS             complement(8651..8839)
                     /gene="dsrB"
                     /locus_tag="POB34_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HDI5920341.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            9002..9229
                     /gene="yodD"
                     /locus_tag="POB34_003456"
     CDS             9002..9229
                     /gene="yodD"
                     /locus_tag="POB34_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HDI5920342.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            9527..10345
                     /locus_tag="POB34_003457"
     CDS             9527..10345
                     /locus_tag="POB34_003457"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HDI5920343.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQQLSGKRPTTLGLGDGPN
                     DAPLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(10342..12036)
                     /gene="yedQ"
                     /locus_tag="POB34_003458"
     CDS             complement(10342..12036)
                     /gene="yedQ"
                     /locus_tag="POB34_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HDI5920344.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFMRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRLLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDIAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(12207..>12285)
                     /locus_tag="POB34_003459"
     CDS             complement(12207..>12285)
                     /locus_tag="POB34_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HDI5920345.1"
                     /translation="YGVKREAFHETELVPGEGSRSAEEV"
BASE COUNT         2976 a         3172 c         3214 g         2923 t
ORIGIN      
        1 cttgagcagc aactggcgca actgcaaatg caggcccatg agcaaggtta tcaggcgggg
       61 attgccgaag gtcgccagca aggtcatgag cagggctatc aggaaggact ggcccggggg
      121 ctggagcaag gtctggcaga ggcgaagtct caacaagcgc caattcatgc ccggatgcag
      181 caactggtca gcaaatttca aactaccctt gatgcgcttg atagtgtgat tgcttcgcgc
      241 ctgatgcaga tagcgctgga ggcggcacgt caggtgatcg gtcagacgcc aacagtggat
      301 aactcggcac tgatcaaaca gatccaacag ttgttgcagc aagaaccgtt attcagcggt
      361 aaaccacagc tgcgcgtgca cccggatgat ctgcaacgtg tggatgatat gctcggcgca
      421 accttaagtt tgcatggctg gcgcttgcgg ggcgatccca cactccatcc tggcggctgt
      481 aaagtctccg ccgatgaagg cgatctcgac gccagtgtcg ccactcgctg gcaagaactc
      541 tgccgtctgg cagcaccagg agtggtgtaa tgaccacgcg cctgactcgc tggctaacca
      601 cgctggataa ctttgaagcc aaaatggcgc agttgcctgc ggtacgtcgc tatgggcgat
      661 taacccgcgc taccgggctg gtgctggaag ccaccggatt acaattgccg ctcggcgcaa
      721 cctgtgtcat tgaacgccag aacggcactg aaacgcacga agtagaaagc gaagtcgttg
      781 gctttaacgg tcaacggctg tttttaatgc cgctggagga agtcgaaggt gtcctgcccg
      841 gcgcgcgggt ttatgccaaa aacatttcgg ctgaaggact gcaaagcggc aagcagttgc
      901 cgctcggtcc ggcgttatta ggtcgcgttc tggacggcag cggtaagccg ctcgatggcc
      961 tgccctcccc cgatacgacg gaaaccggtg cgctgattac cccgccattt aacccgttgc
     1021 aacgtacacc gattgaacat gtgctggaca ccggcgtgcg cccaatcaat gccctgctta
     1081 ccgttgggcg tgggcagcgt atggggctgt ttgccgggtc tggcgttggt aaaagtgtgc
     1141 tgctggggat gatggcccgt tacacccgcg ccgatgtcat tgtcgtgggt ttgattggtg
     1201 aacgtgggcg cgaagtaaaa gattttattg agaatatcct cggtgccgag ggacgtgcac
     1261 gctccgtggt gattgccgct ccggcggatg tctctccgct tctgcgaatg caaggtgccg
     1321 cctatgccac gcgaattgcc gaagattttc gcgatcgtgg tcagcatgtg ttgctgatta
     1381 tggactccct cacccgctac gcgatggccc agcgtgaaat tgcgctggcg attggcgaac
     1441 cacctgccac taaaggttat ccaccgtcgg tgtttgccaa attaccggca ctggtcgagc
     1501 gtgccggaaa tggcattagc ggcggcggat cgattactgc attttatacc gtgctcactg
     1561 aaggcgatga ccagcaggac cccattgccg actctgcgcg ggcaatcctc gacggtcaca
     1621 ttgtgctgtc tcgccgactg gcggaagccg ggcactatcc ggctatcgat attgaagcgt
     1681 cgatcagccg cgcaatgacg gcgttgatca gtgagcaaca ttacgcgcga gtgcgcacct
     1741 tcaaacagct gttgtcgagt tttcagcgta accgcgatct ggttagcgtc ggcgcgtatg
     1801 ccaaaggcag cgatccgatg ctcgacaaag ccatcgccct gtggccgcag ctggagggct
     1861 atttgcaaca aggcattttt gaacgcgcgg actgggaagc gtctctccag gggctggagc
     1921 gtattttccc gacagtgtca taacccagga gataacggca gatggcagaa catggcgcgc
     1981 tggcgaccct gaaagatctg gcagaaaaag aggtagagga tgccgcgcgc ctgctgggtg
     2041 aaatgcgtcg cgggtgtcag caggcggaag aacagctcaa aatgctgatc gattatcaga
     2101 atgaatatcg caataacctc aacagcgata tgagtgccgg gatgaccagc aaccgctgga
     2161 tcaactatca gcagtttatc cagacgctgg aaaaagccat tactcagcat cgccagcaac
     2221 ttaatcagtg gacgcagaaa gttgacattg ccctgaacag ctggcgagaa aagaaacaac
     2281 gtttgcaggc ctggcagaca ctgcaggaac ggcaatccac ggcggcactg cttgcagaaa
     2341 accgcctcga tcagaaaaag atggatgagt tcgcccagcg cgccgccatg aggaaacctg
     2401 aatgattcgc ttagcgccct taattaccgc cgacgttgac accaccacat tgcctggcgg
     2461 caaagccagc gatgctgcac aagattttct cgcgttgttg agcgaagcat tagcaggcga
     2521 gacaactacc gacaaagcgg ccccccagtt gctggtggca acagataagc ccacgacaaa
     2581 aggcgagccg ctggtcagcg agattcttgc cgatgcgcaa caagcggatt tactgatccc
     2641 tgtggatgaa acaccgcctg tcatcaacga cgaacaatcc acatcaacac cattaaccac
     2701 cgctcaaacg atgacgttgg ctgcggtggc tggcaacaat acggcaaaag acgaaaaagc
     2761 ggatgatctg aatgaagacg tcaccgcaag cctgagcgcc ctttttgcga tgttgccggg
     2821 ttttgacaat acgcccaaag tgactgatgt gccgtcaacc gtgttaccgg cagagaaacc
     2881 aacgctcttc acaaaactga cttctgcgca actcacaaca gcacagcctg atgatgcccc
     2941 cggcacgcca gctcagccat taacaccgct ggtagcagaa gcccagagta aagcggaagt
     3001 catcagcaca ccttcaccgg tgaccgctgc cgccagcccg ctaatcactc cacaccagac
     3061 acagccactg cccaccgtcg ccgcgcctgt gttgagtgca ccgctgggtt ctcacgaatg
     3121 gcaacattca ttaagccagc atatttcgct gttcacccgc caggggcaac aaagtgcaga
     3181 gttgcgtctg cacccgcagg atttaggtga agtgcaaatc tccctcaaag tggatgataa
     3241 ccaggcgcaa atccagatgg tttcaccgca tcaacacgta cgcgccgccc tagaagcagc
     3301 gcttccggta ctgcgaacgc agctggccga aagtggcatt cagttagggc aaagcaacat
     3361 cagtggcgaa agctttagtg gtcagcagca ggccgcttcc cagcaacagc aaagccaacg
     3421 cacagcaaac catgaacctc tggcggggga agacgacgat acgcttccgg ttcccgtctc
     3481 tttacaaggg cgcgtaacag gcaacagcgg cgttgatatt ttcgcctaac gtcagaggta
     3541 gcaccgtaat ccgcgtcttt tccccgcttt gttgcgctca agacgcagga taattagccg
     3601 ataagcagta gcgacacagg aagaccgcaa cacatgactg attacgcgat aagcaagaaa
     3661 agcaagcgat cgctttggat cccgattctg gtattcatta ccctcgcggc ctgtgccagc
     3721 gcaggttaca gctactggca ttcgcatcag gttgccgctg acgacaaagc gcagcaacgc
     3781 gtcgtgccct ccccggtctt ctatgcgctg gataccttta cggtcaatct gggcgatgcg
     3841 gatcgcgtgc tgtatatcgg cataaccctg cgcctgaaag atgaagctac acgctcgcgg
     3901 ctgagtgagt atttgccgga agtccgtagc cgtttgctgt tactgttttc gcgtcaggat
     3961 gctgccgtac tggcgacaga agaaggcaag aaaaacctga ttgccgagat taaaaccacg
     4021 ctttccaccc cgcttgttgc cgggcaaccg aaacaggatg tcaccgacgt gctgtatacc
     4081 gcttttattc tgcgataacg acatgggcga tagtattctt tctcaagctg aaattgatgc
     4141 gctgttgaat ggtgacagcg aagtcaaaga cgaaccgaca gccagtgtta gcggcgaaag
     4201 tgacattcgt ccgtacgatc cgaataccca acgacgggtt gtgcgcgaac gtttgcaggc
     4261 gctggaaatc attaatgagc gctttgcccg ccattttcgt atggggctgt tcaacctgct
     4321 gcgtcgtagc ccggatataa ccgtcggggc catccgcatt cagccgtacc atgaatttgc
     4381 ccgcaacctg ccggtgccga ccaacctgaa ccttatccat ctgaaaccgc tgcgcggcac
     4441 agggctggtg gtgttttcac cgagtctggt gtttatcgcc gtggataacc tgtttggcgg
     4501 cgatggacgc ttcccgacca aagtggaagg tcgcgagttt acccataccg aacagcgcgt
     4561 catcaaccgc atgttaaagc tggcgcttga aggctatagc gacgcctgga aggcgattaa
     4621 tccgctggaa gttgagtacg tgcgttcgga aatgcaggtg aaatttacca atatcaccac
     4681 ctcgccgaac gacattgtgg ttaacacgcc gttccatgtg gagattggca acctgaccgg
     4741 cgaatttaat atctgtctgc cattcagcat gattgagccg ctacgggaat tgttggttaa
     4801 cccgccgctg gaaaactcgc gtaatgaaga tcagaactgg cgcgataacc tggtgcgcca
     4861 ggtgcagcat tcacagctgg agttggtcgc caactttgcc gatatctcgc tacgcctgtc
     4921 gcagatttta aaactgaaac ccggcgacgt cctgccgata gaaaaacccg atcgcatcat
     4981 cgctcatgtt gacggcgttc cggtactgac cagtcagtac ggcaccctca acggtcagta
     5041 tgcgttacgg atagaacatt tgattaaccc gattttaaat tctctgaacg aggaacagcc
     5101 caaatgagtg acatgaataa tccggccgat gacaacaacg gcgcaatgga cgatctgtgg
     5161 gctgaagctt tgagcgaaca aaaatcaacc agcagcaaaa gcgctgccga cgcggtgttc
     5221 cagcaatttg gcggtggtga tgtcagcgga acgttgcagg atatcgacct gattatggat
     5281 attccggtca agctgaccgt cgagctgggc cgtacgcgga tgaccatcaa agagctgttg
     5341 cgtctgacgc aagggtccgt cgtggcgctg gacggtctgg cgggcgaacc actggatatt
     5401 ctgatcaacg gttatttaat tgcccagggc gaagtggtgg tcgttgccga taaatatggc
     5461 gtgcggatca ccgatatcat tactccgtct gagcgaatgc gccgcctgag ccgttagtga
     5521 tgaataacca cgctactgtg caatcttccg cgccggtttc tgctgcgcca ctgctgcagg
     5581 tgagcggcgc actcatcgcc attattgccc tgatcctcgc tgctgcctgg ctggtaaaac
     5641 ggttgggatt tgcccctaaa cgcactggcg ttaacggtct gaaaattagc gccagtgctt
     5701 cgctgggcgc gcgtgaacgg gttgtggtgg tcgatgtgga agatgcacgg ctggtgctcg
     5761 gcgttaccgc agggcaaatc aatctgctgc ataaacttcc cccttcagca ccaacggaag
     5821 agataccgca gcccgatttt cagtcggtca tgaaaaactt gcttaagcgt agcgggagat
     5881 cctgatgcgt cgtttgttgt ctgtcgcccc tgtccttctc tggctgatta cgcccctcgc
     5941 cttcgcgcaa ctgccgggta tcaccagcca gccgctgcct ggcggtggac aaagctggtc
     6001 gctcccggtg cagacgctgg tgttcatcac ctcgttgacg tttattccgg caattttact
     6061 gatgatgacc agtttcaccc gcatcattat tgtttttggt ttattgcgta acgcgctggg
     6121 aacaccctcc gcgccaccta accaggtatt gctggggttg gcgctgtttt tgaccttttt
     6181 tattatgtcg ccggtgatcg acaaaattta tgtcgatgcg taccagccat tcagcgaaga
     6241 gaaaatatca atgcaggagg cgctggaaaa aggggcgcag ccgctgcgtg agtttatgct
     6301 gcgtcagacc cgtgaggcgg atttagggtt gtttgccaga ctggcgaata ctggcccgtt
     6361 gcagggacct gaagccgtgc cgatgcgcat tttgctcccg gcctacgtga ccagcgagtt
     6421 gaaaaccgca tttcagatag gcttcacgat tttcatccct tttttgatta tcgacctggt
     6481 gatagccagc gtgttgatgg cattagggat gatgatggtt ccccccgcca ccattgctct
     6541 gccctttaaa ctgatgctgt ttgtactggt ggatggctgg caattgctgg tcggttcgct
     6601 ggcgcagagc ttttacagct agagaggcaa aatgacacct gaatcggtca tgatgatggg
     6661 gactgaagcg atgaaagtcg cgctggcact ggctgccccg ctattgttgg tagcgttggt
     6721 cacgggcctt atcatcagta ttttgcaggc cgccacccag attaacgaaa tgacgctgtc
     6781 gtttattccg aaaatcatcg ccgtatttat tgccattatt attgccggac cgtggatgct
     6841 caatctgttg ctggattacg tcagcacctt gttcactaac ctgccgtata tcatcgggta
     6901 gccgtactat gttgcaggtg acaagcgatc aatggctttc ctggctaagt ctgtacttct
     6961 ggccgttact gcgcgtgctg gcgctcattt ctaccgcgcc aattctgagc gaacgcagcg
     7021 tgccgaaacg ggtaaaactg ggtctggcaa tgatgatcac gttcgctatt gccccatcat
     7081 tacctgccaa cgatgttcct gttttttcgt tctttgctct gtggctggcc gtgcagcaga
     7141 tcctgatcgg cattgcgctt ggtttcacca tgcaatttgc ctttgccgct gtgcgaaccg
     7201 ctggcgaaat tatcggtctg caaatggggc tgtcatttgc gacgtttgtc gatccggcca
     7261 gccatctcaa tatgcctgtt ttagcgcgta tcatggatat gctgacgtta ctgctgttcc
     7321 tgacatttaa cggtcattta tggttgattt cactgctggt cgataccttt cacaccctgc
     7381 caattggtgg cgaaccgttg aacagcaatg cgtttctggc actcaccaaa gcagggagtt
     7441 tgattttcct taacgggctg atgctggcgt taccgctcat tactctgctg ctgaccctga
     7501 atctggcatt aggtttactg aatcgtatgg ccccgcaatt atccattttt gttattggat
     7561 ttccattaac tctgactgtc ggtatctctt taatggcggc attaatgccg ttaattgcac
     7621 ctttttgcga acatttattc agtgagatgt ttaatttgct ggctgatatt attagtgaat
     7681 tgccattaat ataattccgt aacgtttatc atgttatcct aaggattatc cgaaaaataa
     7741 tacctacgaa catcttccag gatactcctg cggcgaaata tttgttttaa gctcactcac
     7801 atatcgcaac atttacttta ctttaagaca attccaggca aattatacaa cactttacgg
     7861 gatagtaagt ccgcctgaaa aatcgcgaga gtggcgcatt aggtgaccca tgttgttccg
     7921 tttagtcatg atgaaatatt caggtaaggg gaattatcgt tacgcattga gtgagggtat
     7981 gccatgtcaa cgattattat ggatttatgt agttacaccc gactaggttt aaccgggtat
     8041 ctgttgagta gaggggttaa aaaaagagaa atcaacgaca ttgaaaccgt tgatgacctt
     8101 gccatagctt gtgattcaca gcgcccttca gtggtgttta ttaatgagga ctgtttcatc
     8161 catgatgctt ctaacagtca gcacatcaag cacatcatta atcaacatcc caatacgtta
     8221 tttatcgttt ttatggcaat tgccaatgtt cattttgatg aatatctatt ggtcagaaaa
     8281 aatttattga tcagttctaa atcgattaaa ccggaatctc tcgacgatat ccttggcgat
     8341 attctgaaaa aagagacaac gataacctct tttttaaata tgccgacgtt atcattgagc
     8401 cgaaccgaat cgagtatgtt gcgaatgtgg atggcaggtc agggaacgat tcaaatctct
     8461 gaccaaatga atatcaaagc caagaccgtt tcatcgcata aaggtaatat taaacgtaag
     8521 atcaaaacgc ataataaaca ggttatctac catgtcgtcc gactgacgga taatgtgact
     8581 aatggtattt ttgtcaacat gcgctaacac attctgactg gtggtttccc accagtcagg
     8641 ctgaataaga ttactctgct ttctccacaa agataccgtc ctgatgccct gcttcattaa
     8701 agaaccagat gccgagcggg tagtcttcca gcgaaaccag gtacattgtg ccttcactaa
     8761 actcctcaac tgccagtacc acgccaggac gacgcggacc gccatccgtt ttgactgtta
     8821 cccgatcatt caccttcatc gttttcctcc tgtggctttg tgccagtgta gaacaatttc
     8881 gtttttctgg cagcgccagg cgcgcgcgag tgctgatttt ctcgacggtc tatacttaag
     8941 agatgccagg cggacttaac gactggcggc aacaacagag taacggttgc gaggaaagat
     9001 gatgaaaacc gcaaaagagt acagcgatac cgcaaaacgt gaggtcagcg tcgatgtcga
     9061 tgccctgctg gcggcgatca atgaaattag cgaaagcgaa gttcatcgca gccagaacga
     9121 ttctgaacac gttagcgtcg atggacgtga atatcataca tggcgtgaat tggcggatgc
     9181 cttcgaactg gatattcatg acttcagcgt ctctgaagtg aatcgttgaa tgcacaataa
     9241 aaaaatcccg accctgaggg ggtcgggatg aaacttgctt aagcaagaag cacttaaaaa
     9301 attcgttaca ccaggaaatc tgatgtgttc atcaccttat ccgcaatttt tttcgctgac
     9361 aagaaaatat tcgcgatatg aatgattaac gctacttatg aatagaaatg tgacgtgcgt
     9421 caccccttgg tggctctggc gtcagcggga tcgctcccga aaaaatcatc tttgataatt
     9481 tgtgctgttt tgagaatcat cctggtaagg ggagtactgc gcgactatgt tttcaattca
     9541 acaaccacta ctggttttta gcgatcttga tggcaccctg ctggacagtc atagttatga
     9601 ctggcaacca gctgccccct ggctcagccg tttacgcgaa gcgaatgttc ccgtcattct
     9661 ctgtagcagt aaaacttcag cggaaatgct gtacttgcaa aaaacgttgg ggctacaagg
     9721 gttaccgctg attgccgaaa atggcgcagt gatccagctt gctgaacaat ggcaggatat
     9781 agacggtttt ccacgcatca tctcaggtat tagccatggc gaaatcagcc aggttttaaa
     9841 tacgctacgc gagaaagaac attttaaatt cacaactttt gatgatgtcg acgatgcaac
     9901 catcgccgaa tggacgggat taagccgtag ccaggcggcg ctgacgcagc tgcatgaggc
     9961 gtcggtaacg ctaatctggc gcgacagtga cgagcgtatg gcacaattta ccgctcgtct
    10021 gaacgaactg ggcttgcagt ttatgcaggg tgcgcgcttc tggcacgtac tggatgcctc
    10081 tgccggaaaa gatcaggctg ccaactggat tatcgcgacc tatcaacaac aattgtcagg
    10141 caaacgccca accacacttg gcctgggcga tgggccaaac gatgcgccct tactggaggt
    10201 aatggattac gcggtgattg tgaaagggct aaaccgtgaa ggggtgcatc tgcatgatga
    10261 ggatccggcc cgcgtctggc gaacgcagcg tgaaggaccg gaaggatggc gtgaagggct
    10321 ggaccatttt ttctccgccc gttaagcgtt atcgctcgcg catacccgat tacgcccggc
    10381 ctgtttagcg agataaagtc gacggtcagc cagtgactgg agttgttcaa aatcataatc
    10441 accggtttcc tcgctgctgc ttacccccag cgaggcactg atgcgtatcg tcgtactctt
    10501 ggcgatcaac atctcttttt catttaattt cagtcgaata cgttctgcga cctccgcagc
    10561 ctccgtcaga ctcgcgcctg gcagaatcac acaaaactcc tcaccaccga cccgcccggc
    10621 aatgtcctgc gcacgcaagg aactgctaat taatccggca gcatgagaaa gaacacggtc
    10681 gcccgcctga tgaccaaagc ggtcattaat cgctttaaaa tggtcaaggt cgacctgaat
    10741 gacagaaaaa ggatgttggt gtgtctgaca caatttggcg agcggacggg ctttttcgaa
    10801 cagtgcgcca cgattatata aacgggttaa ggtgtcgtgc cacgcctgcc actgcaacga
    10861 gctttgcaga acatacatgt tgctgaccat ccggcgaatc acataccagg agatgagtaa
    10921 catggtggta aagagcgccc acagcagggt taatgcaatg ctgatactgc cgaaatcgcc
    10981 gcgcacgcct tcgcttagcg tatggacgcg caccagcaca ccgtcgaaat ggtccagacg
    11041 ttcccagcta acatagcgac tgttcatacg aatgccgccc cgtgtgtcat gttccattgc
    11101 ctgcgccagc aaggccagtt cacgaggatc aaaaatattc cctgttggat gatcaggatt
    11161 ggaagaggtc aataatctca gcttactgtc atagagctga tactcaccat cgaggttttt
    11221 atcgatggcg tttcttaaaa attgctgcat ggtacgcacg ggaatactca tccccagcac
    11281 gccataccag tagttattac tgtctaccgg aacactgacg gtaacctgcg gttcagtatt
    11341 gctggcgtgt tccggttgcg aagtgaacca gcgtaccgcg cggtgacgat tttctcgttg
    11401 tgaatggccg ataaaccagg gttgggtgac atagccgtaa taacgcgtcg ggacattgcg
    11461 cataaacaag gtcggctgcg tcgaaacgta aaatccggca cgcgagacat acatcgcctg
    11521 ttcaaccatc gacgaggagt tgtgtgccag tcgcagcaag taaccaactt ccagtgcagc
    11581 ggtaatttca ttgtcgaggc tttcattttc gcgagacagg agattcccct cgctgactaa
    11641 tgcatccgat acgccattga ctgacagggt gcgtcgtcgg ttgagttcaa tttgccaggc
    11701 gtgctcgtcg cgatgctgtt cgaactcggt tacagcatta cgcagtgaag tgaaatccag
    11761 tggcgctacg agagcttcgc gcatgccatt acgcaaaaag atcagtttgt cgacgttata
    11821 ctgcaaatgc ttatcgagcg cgttggctac gttttccaga tgattacgct ggctggagat
    11881 ataggcatct tccagcacca ctacttcacg ccaggtgagc aaggtggaaa aaagcaatac
    11941 cacgataaag cagagattaa cgacatgacc aggccccagg cggcgtgcga gttttttcaa
    12001 ccagctctgg ttttccattt ttgtctcgtg ctgcaccctg acgactccca acctgctttt
    12061 ttatgattct ggcataacct ggcgatagcg taccgcaaaa cagcgataat tttataatgg
    12121 tggcatgata agacgcgact atcattcatt gcaggcattg gcacaccagg ccggatgtga
    12181 tccggcctgg tgttaatgat accctttcaa acttcttccg cagaacgact cccctccccc
    12241 ggaacaagct cggtttcatg aaaagcttcc cgcttgaccc cgtaa
//
LOCUS       DAMZET010000124        12220 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.124, whole genome shotgun sequence.
ACCESSION   DAMZET010000124 DAMZET010000000
VERSION     DAMZET010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.124"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..728
                     /locus_tag="POB34_003460"
     CDS             <1..728
                     /locus_tag="POB34_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HDI5920346.1"
                     /translation="QAVQEIKHSLDHGRKTGGRLLMFGVGVIVIGVMLSILQQFVGIN
                     VVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIMTVDKFGRKPLQIIGALGM
                     AIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCWVLLSEIFPNAIRGKALAI
                     AVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGCMGVLAALFMWKFVPETKG
                     KTLEELEALWEPETKKTQQTATL"
     gene            complement(772..1182)
                     /gene="psiE"
                     /locus_tag="POB34_003461"
     CDS             complement(772..1182)
                     /gene="psiE"
                     /locus_tag="POB34_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HDI5920347.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(1308..1451)
                     /locus_tag="POB34_003462"
     CDS             complement(1308..1451)
                     /locus_tag="POB34_003462"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920348.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     VYG"
     gene            1452..1730
                     /locus_tag="POB34_003463"
     CDS             1452..1730
                     /locus_tag="POB34_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920349.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(1777..3873)
                     /locus_tag="POB34_003464"
     CDS             complement(1777..3873)
                     /locus_tag="POB34_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HDI5920350.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3873..4610)
                     /locus_tag="POB34_003465"
     CDS             complement(3873..4610)
                     /locus_tag="POB34_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HDI5920351.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEYLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(4607..5245)
                     /locus_tag="POB34_003466"
     CDS             complement(4607..5245)
                     /locus_tag="POB34_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HDI5920352.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(5359..5601)
                     /locus_tag="POB34_003467"
     CDS             complement(5359..5601)
                     /locus_tag="POB34_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920353.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(5956..7605)
                     /gene="pgi"
                     /locus_tag="POB34_003468"
     CDS             complement(5956..7605)
                     /gene="pgi"
                     /locus_tag="POB34_003468"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDI5920354.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRD"
     gene            8130..9479
                     /gene="lysC"
                     /locus_tag="POB34_003469"
     CDS             8130..9479
                     /gene="lysC"
                     /locus_tag="POB34_003469"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HDI5920355.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAAVQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            9624..10550
                     /locus_tag="POB34_003470"
     CDS             9624..10550
                     /locus_tag="POB34_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDI5920356.1"
                     /translation="MATLMEKDALLNGASQCIAFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGAGKSSLIELAKRETKDNIMIINGDDFRFLHPDFNHIYQNYGD
                     DFVTHTAKFSGETVERAIERAIVSKLNIVVEGTFRNAATPLQTLKKLKDAGYQTEVMI
                     KTTSAALSWESTNERYNKDKEAGNIARKVDKNHHDIVTGLLAENARKVFASNLADKFA
                     VYSREKMIFSSQAATNDDIATLIQNEISGNTQ"
     gene            complement(10586..11377)
                     /locus_tag="POB34_003471"
     CDS             complement(10586..11377)
                     /locus_tag="POB34_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDI5920357.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVESPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITAFKAMVPMWFSPLRARWHYYSMAQKNFPGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAELARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(11383..>12220)
                     /locus_tag="POB34_003472"
     CDS             complement(11383..>12220)
                     /locus_tag="POB34_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HDI5920358.1"
                     /translation="RRLYWRVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRD
                     AILTVQVPAWHVGVLKIDGETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEI
                     LTKDKVNLRINLAANWRYSDVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLED
                     KQVIDDVVSEQVKSRMLPFGMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRR
                     REETAATRSLLNTAKVMENNPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNI
                     KG"
BASE COUNT         2940 a         3244 c         3025 g         3011 t
ORIGIN      
        1 ctcaggcagt acaggaaatt aaacactctc tggatcatgg ccgcaaaacc ggtggtcgtc
       61 tgctgatgtt tggcgtaggc gtgattgtca tcggcgttat gctctccatc ctccagcaat
      121 ttgtcggcat caatgtggtg ctgtactacg cgccggaagt gttcaaaacg ctgggggcca
      181 gcacggatat cgcgctgttg cagaccatta ttgtcggagt tatcaacctc accttcaccg
      241 ttctggcaat tatgacggtg gataaatttg gtcgtaagcc actgcaaatt atcggcgcac
      301 tcggaatggc aatcggtatg tttagcctcg gtaccgcgtt ttacactcag gcaccgggta
      361 ttgtggcgct tctgtcgatg ctgttctatg ttgccgcctt tgccatgtcc tggggtccgg
      421 tatgctgggt actgctgtcg gaaatcttcc cgaatgctat tcgtggtaaa gcgctggcaa
      481 tcgcggtggc ggcccagtgg ctggcgaact acttcgtctc ctggaccttc ccgatgatgg
      541 acaaaaactc ctggctggtg gcccatttcc acaacggttt ctcctactgg atttacgggt
      601 gtatgggcgt tctggcagca ctatttatgt ggaaatttgt cccggaaacc aaaggtaaaa
      661 cccttgagga gctggaagcc ctctgggaac cggaaacgaa gaaaacacaa caaactgcta
      721 cgctgtaatc ttcctgtcca gcacgccgcg ccagttcggc gtgctgtctt tttactcccg
      781 cttcagccgt ttcgaattac acagccacag ggtgatcacc agtaacagga tcgcagccga
      841 gtagatcaac acatccagtg gcgatttatg atcgacgatg atcaagcgca caatggcggt
      901 gatcccaata tagacaaagt aacgtagcgg gaagtgaaaa ccggactgaa agtacttcac
      961 aatcagcgcg ataaattcga aatagagaaa gtaaaccacc agtccttcta ccagctcata
     1021 tttgctggtt tgttctggcg cgaatagcac atcagccaga tgcaccgttt ctttgccgag
     1081 gaagacaacc aaaatcaggc cgaggcacaa caggccaaga ttgagtacgg tctgcaaaat
     1141 ggtggagata aactccacgc gcggacgaga gagtgacgtc atagcagtac ctccttcaca
     1201 gtggcgagct tcctgtataa cgcaaaaatg tgacggagat ctatattttt tgtgtatgtt
     1261 ttgttcaacc tggatttgga gcgcgcctcc accaaagccg gatataccta cccgtagacc
     1321 ggataagccg cgcctggcgg cgcatccggc aagacaaacc gcaccaaagc cggtaattca
     1381 aaaatcaccc attcaggtga ttcccagcca ccagcaatgc gcgaaaaatc gacaatgttc
     1441 ctctggaaca aatgaaacgt aacctgataa aggtggtaaa aatgaagcct tattttgctg
     1501 ctttgatgtt atcagtctct gttcttcctg cttatgctgg tccgctcggt actgctgata
     1561 aagccgacct accgcaaagc aatgtctcca gcccaatgat ggcccagtcg ctcaggcaac
     1621 cagacctgca accaatatcg acggacagga agacagaatg ttttcgcctt tacacaccag
     1681 atcgcaaacc gggggtgaac tgcgttcctg atggctcgac aggccattaa gaatgaaggt
     1741 gccagtgagt gcgaaaactg gcacctttcc catgacttag cggaaattta cgctacggtc
     1801 gctggtcatg tcatacaact ggaacttacg cccaagttgc tgaccaccgt cacgcgtcag
     1861 cggcgtccag ccaattgccg cacggctgcg tgtcgggcca gacgagaaga gatccaacgg
     1921 taccgagaca tacacgcctt tggtgaagtc cccttcgccg tactcctctt tcgaaacatt
     1981 cgtgatcgtg gcatagccac ccaccaccac accgctatca aagcgtttgg cgatctccag
     2041 cgtgccgcct ttatcccctg ccagatactg cccgacgctg gctttaacta acacatcctg
     2101 agcgaaagat ggcgtccagt aggcggtcag atgtccggtt ttcacgctgt agtcggtgaa
     2161 tttcatcata tcttttgcac tacgccagtc gcgctgttta acgtagttgg catccagacc
     2221 aaacgcccag ttgctgtcca gcgggcgata caacacctca gcccccgcac cgccaaacat
     2281 ggtttcgaga tagccgccgt agacctgacc gtagaagccg ttgcccagat gctggaagta
     2341 gttggcttgc aggttattca catagacatc gttctgcaca tactcgcgca catgggtacg
     2401 cacgcgcggt aagtgcgagt cctgcggcgg attggtgtag ttaaacttgt cgtagttgtt
     2461 ggcaagattt gcaaacaggc tgccggtggt cagcagatgg tccgtcagcc acaaatctgc
     2521 cgttcccatc acgcccagct gatacatgta aaagttttcc gggccaccga ccgactggtt
     2581 cagcaccgga tcgatatgga aatcaaagcg cgatttgtcg atataccagc cctgctcggt
     2641 ggacttcgga accactggct cgacgcgttt ttgcgccagc ttcgtttcgt ggcccaacgg
     2701 ctctccggcg agatgatttt tcaggctggc gacatccgtt tccgtcgtca cctgcggcat
     2761 gttaaggcga ttttccgtaa tgcggatcgt tttgatccca tccggcagat cgttcatcac
     2821 gatccgatta gcacggatga tcccttcgcg cgaatcacga tatttcacct gctcgccggt
     2881 aacatacagc gtatcgcctt tcgcctggat ctgtggatcg gcaagtccgg cattgtattt
     2941 caacagcgtt aactgattcg ccaccaccga atgctgcaaa atggcatcct gcggctgcgg
     3001 ttgatattgc gggcgggcgt tatcgttgta agacgggcgc agatcgttaa agttggtgcg
     3061 caacgtaacg ccaaacataa aggtgttgcc acgttcatag ctaaggttaa cgtcggccca
     3121 atcggtaacg cgataaatcg caccgacgtt aaacttactt ttttgctcca gcttcccggc
     3181 aaaatcctgc tgataattat tgccttcata ctccagtttc aggcgcagcg gttgccaggg
     3241 cgtctggtat tccacgccgc caaacagtga ggcaggaccg tggaacatct ggctaccgtc
     3301 gatagatccc gcctgtttgt agctgttatc gcgatagcaa tatttatcac tggctgaaca
     3361 gagcggattt ttcacattac cgctggtgcc cagatatccc cagcccaggc cgagcgtaaa
     3421 atcgaacggc ccccaggctt tgctggcaac aagatattcc gcatcaaaca gccccgtacc
     3481 gccgatatcc cgcgcaccaa ccgccacttg cggcagccag taactctctt cccacaaacg
     3541 cagtttgaga tcgaaggctt tatctttata cgtttgatcg ccagagaacg cttcgacgct
     3601 gctgtactgc cgggtgcgca cgtcggtgta gcgcagcgtt gtttccagcc acgggaagag
     3661 ttgcactgaa gctgagtaat aacggtactg atcgttatct cgatagttca ggctcaactc
     3721 cccttcccgc gccatgcgcg cggtgggcgt ttgtaataat cctacgccac cgaaatccga
     3781 ctgcgacgga ccaatgggcg caggatatgt ttcgccgtag caagctgtgc taatgcccag
     3841 cgccagtaag ctaagcagat gtcttttttt cattattggg gtatccgctg cgtcagagtc
     3901 tgaagaatgt cggcgttaag ggcatcaggc gtctcactcc agacggagtc cgcgaggcca
     3961 acataaataa tgctgccggg catcggctct acgtgacgct tgttccagta agccaccggc
     4021 gctttttgtg tgcgtccgtc cgggtaaacg acccacgcgt agctgcgatc cgcaccgctg
     4081 agcaggctct ggtcagagag atagctcgcc acgtcgcgac ctggagtgaa tggctgcttg
     4141 ccaggacggc tgataagccc gaacagcgtg accgtggacg gtggtggccc gacccacagc
     4201 gtgtagttgc cctgcaacgg tgggttaccg cgttcggcaa cgcggacgat atcaggatca
     4261 agattgattt tttgcctgcc cgtcactttc aacgcctgaa tttgctggcg taaggcgtta
     4321 atcgcggcgg catcgtcggc gctggaatct gcagccagtt ctgccagccg tgtcagcaac
     4381 gcctgctgct gccgcaatgc tgccgccgtt gccagctctt cgctaatcac cgcaccaggc
     4441 caccagctat tcgccagccg cggctggcct accagatcga ttaaatactc tgcgcccgtc
     4501 agcgttttgg cctcactgct gccattgctg aataccttga cggttcccgc cgcgaagacc
     4561 gatgacgccc ccacactcaa aatcaatgcg acaatagttt gtttaatcat tgttttgccg
     4621 ccttgatgag agtggttttc accgggaaat aatccgcgcc gagatactgt tccgactggc
     4681 gaatttgccc ttcgctgtcg atccagtagc ggttatgcca gcgcgcctgg tcggtggaga
     4741 cttcttcgtc cagcacgcga accggggttt catcgctgcc gaccttgacg gtatcggtgc
     4801 catcccattt gaagactgag cgtgcggtgg cgtagcgtac ctgctggtac tcggtccagc
     4861 ccatcgtgcg cgtccatgtt gcaccatcaa caatttgtgc gggtttaatc agcgggtcgg
     4921 cggcaaggtt attcacttca atcaggttgt cgccgccaag caaagtcttc accagacgac
     4981 catgttgtgt gacaagagtg gcttgatcct gagtgaccca tttttgttgt ccgtcttcag
     5041 cgaaggccag caccacaaac aattgcggcc cgccattaag ctgcatgtac tggctggcgt
     5101 agggcatatt ttgaatatca tcgtccgtca gctgtacgcc tggcgtgccg aacagactgt
     5161 cccacagtga attgcccagc tctttagtcg tggctgaaca ggcctgtaat agcaggcaga
     5221 taagaatgag tgcaggtcgc ttcacgactc ttctccagag gggcagaata accacaccgg
     5281 agtgtggtta tggtaaatcc cttcatactt caagccacag atgcaatgac tcaaattatt
     5341 ttggggatac atactttatt actgggtact ggtggtcgtg gttgtggtgg tcccggtgtt
     5401 agaaccatca ccgccaccgg ttgccgcgag cgcgacaccc accgccgagc ttacggtgct
     5461 gacgctggtc gcggaggaac tccccgccga aaccgacgtt gctgccgacc ctgccgcttc
     5521 gcccacctgt acaggtgcag cccacgcaga agtcgccgca agcgcagata tggcaaaaat
     5581 gccatacaga acttttttca taacaatttc ccttcattga atgaatggag atttacccaa
     5641 aaacatttcg ggcgggatcg agtatacaca actaaagcat gcgaattacg gcgcggggaa
     5701 ttagagatgg ggttttagga taattggaat gaatatcaaa tataagctac aacttatata
     5761 taaataacat taaaaatcat aatgttaaaa taaaagtaaa agtaattaat ctgagtattt
     5821 tccgaaatca atcacgatta aaaatctatt ttaggattaa cctcacggta tgatttccgt
     5881 taaatcacag acaaggggat ttatctgata aaacaatgcc gacataacgt cggcattgtt
     5941 attaaggttt ttaaattaat cgcgccacgc tttatagcgg ttaatcagac cattggtcga
     6001 gctatcgtgg ctgctgattt ctttatcatc tttcagctct ggcagaatac ggtttgccag
     6061 ttgtttaccc agctccacgc cccactggtc gaaggtgaag atgttcagga tcacgccctg
     6121 agtaaagatt ttgtgctcat acagcgcaat caacgccccc aggctgaacg gagtgatttc
     6181 acgcagcagg atggagttgg tcgggcggtt accttcgaac actttgaacg gcaccacgta
     6241 gtcaagcgtt gccggatctt taccctgatc gcgatattcc tgctcaacca cttcgcggga
     6301 tttaccaaac gccagcgctt cagtctgagc aaagaagtta gacagcagtt tctggtggtg
     6361 atcagagagc gggttatggg tgatagccgg agcgatgaaa tcgcacggta ccattttggt
     6421 tccctggtgg atcagctggt agaacgcgtg ctgaccgtta gtgcctggtt caccccagat
     6481 aatcgggcca gtctggtaat ccacaacgtt accgttacgg tcaacatact taccgttgga
     6541 ctccatattg ccctgctgga agtacgccgc gaaacggtgc atatactggt catacggcag
     6601 aatcgcctca gtttccgcac caaagaaatt gttgtaccag atgccaatca gcgccagcag
     6661 tacaggcagg tttttctcgg caggcgtggt ggagaaatgc ttgtccatcg cgtgtgcgcc
     6721 agaaagcagt tcaacgaagt tatcaaagcc gatggagaga acaatcgaca ggccaatcgc
     6781 tgaccacaaa gagtaacggc cgccgaccca gtcccagaac tcgaacatgt tggcagtatc
     6841 aataccaaac tcgccaacgg ctttggcatt ggttgagagc gccgcaaagt gtttcgcaac
     6901 gtgtttttca tcacctgccg ctttcaggaa ccagtcacgc gcgctatggg cgttggtcat
     6961 ggtttcctga gtggtgaagg ttttagatgc taccaggaac agcgtggttt ccgggtttac
     7021 ttttttcagc acttccgcga tgtgagtccc atcgacgtta gaaacaaagt gcatgttcag
     7081 gtggtttttg tacggacgca gtgcttcggt caccatgtac gggccgaggt cagaaccgcc
     7141 gatcccgatg ttcactacgt cagtgattgc tttgccggta taccccttcc actcaccgga
     7201 gataatcgct tccgagaagg ttttcatctt ctccagcacc gcgttgactt ccggcattac
     7261 gtctttgcca tcaaccagaa tcggggtatt gctacggtta cgcagcgcta cgtgcagcac
     7321 ggcgcggttt tcagtgcggt tgatcttctc gccagagaac atcgacttaa tcgcgcccgc
     7381 cagatcgcac tctttcgcca gatcctgtaa tttcgccagc gtctcttcag tgatgcggtt
     7441 tttggagtaa tccaccagca tctgatcgtc gaatgttgcg gagaacttag aaaaacgatc
     7501 gccgtcttta gcaaaaagat cggcgatcgt aacgtctttc atttcatcga agtgtttctg
     7561 tagtgcctgc caggcagcgg tctgcgttgg attgatgttt ttcattagca atactcttct
     7621 gattttgaga attgtgactt tggaagattg tagcgccagt cacagaaaaa tgtgatggtt
     7681 ttagtgccgt tagcgtaatg ttgagtgtaa acccttagcg cagtgaagca tttattagct
     7741 gaactactga ccgccaggag tggatgaaaa atccgcatga ccccgtcgtt gacaaccgcc
     7801 ccgctcaccc tttatttata aatgtactac ctgcgctagc gcaggccaga agaggcgcgt
     7861 tgcccaagta acggtgttgg aggagccagt cctgtgataa cacctgaggg ggtgcatcgc
     7921 cgaggtgatt gaacggctgg ccacgttcat catcggctac aggggctgaa tcccctgggt
     7981 tgtcaccaga agcgttcgca gtcgggcgtt tcgcaagtgg tggagcactt ctgggtgaaa
     8041 atagtagcga agtatcgctc tgcgcccacc cgtcttccgc tcttcccttg tgccaaggct
     8101 gaaaatggat cccctgacac gaggtagtta tgtctgaaat tgttgtctcc aaatttggcg
     8161 gtaccagcgt agctgatttt gacgccatga accgcagcgc tgatattgtg ctttctgatg
     8221 ccaacgtgcg tttagttgtc ctctcggctt ctgctggtat cactaatctg ctggtcgctt
     8281 tagctgaagg actggaacct ggcgagcgat tcgaaaaact cgacgctatc cgcaatatcc
     8341 agtttgccat tctggaacgt ctgcgttacc cgaacgttat ccgtgaagag attgaacgtc
     8401 tactagagaa cattactgtt ctggcagaag cggcggcgct ggcaacgtct ccggcgctga
     8461 cagatgaact ggtcagccac ggtgagctga tgtcgaccct gctgtttgtc gaaatcctgc
     8521 gcgaacgcga tgttcaggca cagtggtttg atgtacgtaa agtgatgcgt accaacgacc
     8581 gatttggtcg tgcagagcca gatgtagccg cgctggcgga actggccgcg gtgcagctgc
     8641 tcccacgcct caatgaaggc ttagtgatca cccagggatt tatcggtagc gaaaataaag
     8701 gtcgtacaac aacgcttggc cgtggaggca gcgattatac ggcagccttg ctggcggagg
     8761 ctttacacgc atctcgtgtt gatatctgga ccgacgtccc gggcatctac accaccgatc
     8821 cacgcgtggt ttccgcagca aaacgcattg atgaaatcgc gtttgccgaa gcggcagaga
     8881 tggcaacttt tggtgcaaaa gtactgcatc cggcaacgtt gctacccgca gtacgcagcg
     8941 atatcccggt ctttgtcggc tccagcaaag acccacgcgc aggtggtacg ctggtgtgca
     9001 ataaaactga aaatccgccg ctgttccgcg cgctggcgct tcgtcgcaat cagactctgc
     9061 tcactttgca cagcctgaat atgctgcatt ctcgcggttt cctcgcggaa gttttcggca
     9121 tcctcgcgcg gcataatatt tcggtagact taatcaccac gtcagaagtg agcgtggcat
     9181 taacccttga taccaccggt tcaacctcca ctggcgatac gttgctgacg caatctctgc
     9241 tgatggagct ttccgcactg tgccgggtgg aggtggaaga aggtctggcg ctggtcgcgt
     9301 tgattggcaa tgacctgtca aaagcctgcg gcgttggcaa agaggtattc ggcgtactgg
     9361 aaccgttcaa cattcgcatg atttgttacg gcgcatccag ccataacctg tgcttcctgg
     9421 tgcccggcga agatgccgag caggtggtgc agaagctgca ttttaatttg tttgagtaat
     9481 atttccctac tggcgtataa cgctcaccga ttgttatacg ccatcctcac tgaaaacata
     9541 gattaaataa tatctgaaaa gcaaaatcac tcctgtagaa cagtattata atcaccgaaa
     9601 aatatcattt aacaggagtg ttaatggcta ctttaatgga aaaagatgca cttttaaacg
     9661 gagccagtca gtgcatcgcg tttttatcaa atatcattga taattgctca gtttcgtcgc
     9721 atcaggattc aggcgatgct ctcaagcgat tagtcagcta tcgcgattat ctttattcta
     9781 cccccgctga acttgttgat tttacacaag gtaagattct ccttcagcag gtaagaactc
     9841 aatatcagca tgaattcaac aatactacgc attcagaaaa taaagcatct tttgattcta
     9901 tttggcagcg tctgacaaat catgaagtca caccgcaaca gcatcccatt ggttttgttt
     9961 taggtgggca acccggagca ggtaaatcat ctctgattga attagccaaa agagagacaa
    10021 aagataatat aatgattatt aacggtgatg attttcgatt tctacaccct gattttaatc
    10081 atatctacca aaattatggg gatgattttg ttacccatac agcgaaattt tccggcgaaa
    10141 cggtcgaacg tgcaattgag agagcgatag tcagtaaact caacattgtt gtcgaaggaa
    10201 catttcgtaa tgccgctacg ccgttacaaa cattaaaaaa actcaaagat gcaggctacc
    10261 aaacagaagt catgataaaa acaacctcag cggcattaag ctgggaaagc accaatgaac
    10321 ggtataataa ggacaaagag gcgggaaata ttgcccgaaa agtggataaa aaccaccatg
    10381 atatcgtcac cggtttgctg gcagaaaatg cccgcaaggt gtttgcttca aacctggcag
    10441 ataagtttgc ggtgtattcg cgtgagaaga tgatatttag ttcacaagcg gcaacgaatg
    10501 acgatattgc aacgcttata cagaatgaaa tcagcgggaa tacacaataa taataagagc
    10561 tgatggcgtg aaataacgcc gggctttatg cccggcgcat taccgtctgg tacattaagc
    10621 tatccagttc cttaacgtcc ccttctctgc tatccggtaa cagcggtgga atttccccac
    10681 gcgccagttc cgcacgaata aacgcatgta acaatggacg gcgcggacca tattccgctt
    10741 ctcctgcgcg ctgtttacgt tccagtaatt cgtctatttc tgcacgtaac ggcgcatcaa
    10801 gttcgcttcc cgccagtaac tcagcaaagc gcattggcgg tacgccttta cccgcttcga
    10861 cccagcgcac ggccagcagc ggacgcagca cgtaaaagta ttttttcagt cgaacttcgt
    10921 cgccctgcaa atatccgggg aagtttttct gcgccatcga atagtaatgc cagcgcgcac
    10981 gcagcggaga aaaccacatt gggaccattg ccttaaacgc cgttatcgtt tcttcatctt
    11041 gttgataaac caccggagaa tccagccatt ctatcagcgt cgggttcgcg cctttcaata
    11101 atcccagcgc cttacgccac tcccaaccgg aaacatctaa ctcgtcgtcg atgggcagct
    11161 caataacatc gcgcggtgac tctactcgca gataccattc cagcgggtga acatacaaaa
    11221 accgtacatc gtagtcgctg tcgggcgagg caaatcccca gccgcggctc cctgattcac
    11281 aggcgtacag tactttcaca tcataccgcc gttcaatttc ttttaactgc tgcgataccc
    11341 tttctcgcat cgcagcactg actccattta atggcatgtt actcaccctt tgatattaac
    11401 caacccatgc agaacctgat ccagaccgcc aaatacagag atgttatcga tccgttcagc
    11461 cactctctcc agcgtttcca gttcttttaa gcgtaacgct accgggttgt tttccatgac
    11521 tttagcggta ttgagtaacg aacgcgtcgc tgccgtttct tcacgtcggc gaatcacatt
    11581 ggcctgcgcg gatttctccg cctcaaccag ctgcgcaagg atatttttca tatcgccagg
    11641 caggacgata tctttcaccc ctaacgaggc aatctccata ccaaatggca acatgcgcga
    11701 tttcacctgt tcgctgacaa cgtcatcaat gacctgttta tcttccagca attcatccaa
    11761 cgtacgcgta cctaccgctt cacgcagggc aaattgtaat tcccgataca aatgatcaat
    11821 cggcttcgtt aactggctaa atgccaataa cacgtcgctg tagcgccagt tagcggcgag
    11881 gttaatccgc agattgacct tatctttcgt taagatttct tgcccgctga cttccagtac
    11941 ctgcaagcgg gtgtcaacca cttcagcgtc aaccagatgg ttaattttcc agtatgcggt
    12001 cagccccggc ggtaacagcg cctgcgtttc accatcgatt ttcagcacgc ctacatgcca
    12061 ggccggaacc tgcaccgtta gtattgcatc gcggccttta actgcccctt tgcgccgtgg
    12121 ttgcaatacc gcattcatca cctccgtttg tacctggact tgctgcgtat tcatccgcac
    12181 cagtgtcaaa tcatcctcta cacgccagta caaacggcgc
//
LOCUS       DAMZET010000125        12150 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.125, whole genome shotgun sequence.
ACCESSION   DAMZET010000125 DAMZET010000000
VERSION     DAMZET010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.125"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..102)
                     /locus_tag="POB34_003473"
     CDS             complement(<1..102)
                     /locus_tag="POB34_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HDI5920359.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQF"
     gene            complement(99..656)
                     /gene="dsbE"
                     /locus_tag="POB34_003474"
     CDS             complement(99..656)
                     /gene="dsbE"
                     /locus_tag="POB34_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HDI5920360.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(653..2596)
                     /gene="ccmF"
                     /locus_tag="POB34_003475"
     CDS             complement(653..2596)
                     /gene="ccmF"
                     /locus_tag="POB34_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HDI5920361.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(2593..3072)
                     /gene="ccmE"
                     /locus_tag="POB34_003476"
     CDS             complement(2593..3072)
                     /gene="ccmE"
                     /locus_tag="POB34_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HDI5920362.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(3069..3278)
                     /gene="ccmD"
                     /locus_tag="POB34_003477"
     CDS             complement(3069..3278)
                     /gene="ccmD"
                     /locus_tag="POB34_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HDI5920363.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(3275..4012)
                     /gene="ccmC"
                     /locus_tag="POB34_003478"
     CDS             complement(3275..4012)
                     /gene="ccmC"
                     /locus_tag="POB34_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HDI5920364.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(4054..4716)
                     /gene="ccmB"
                     /locus_tag="POB34_003479"
     CDS             complement(4054..4716)
                     /gene="ccmB"
                     /locus_tag="POB34_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HDI5920365.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(4713..5330)
                     /gene="ccmA"
                     /locus_tag="POB34_003480"
     CDS             complement(4713..5330)
                     /gene="ccmA"
                     /locus_tag="POB34_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HDI5920366.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(5349..5951)
                     /gene="napC"
                     /locus_tag="POB34_003481"
     CDS             complement(5349..5951)
                     /gene="napC"
                     /locus_tag="POB34_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HDI5920367.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(5961..6410)
                     /gene="napB"
                     /locus_tag="POB34_003482"
     CDS             complement(5961..6410)
                     /gene="napB"
                     /locus_tag="POB34_003482"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HDI5920368.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(6407..7270)
                     /gene="napH"
                     /locus_tag="POB34_003483"
     CDS             complement(6407..7270)
                     /gene="napH"
                     /locus_tag="POB34_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HDI5920369.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(7257..7952)
                     /gene="napG"
                     /locus_tag="POB34_003484"
     CDS             complement(7257..7952)
                     /gene="napG"
                     /locus_tag="POB34_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HDI5920370.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(7959..10445)
                     /gene="napA"
                     /locus_tag="POB34_003485"
     CDS             complement(7959..10445)
                     /gene="napA"
                     /locus_tag="POB34_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HDI5920371.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(10442..10705)
                     /gene="napD"
                     /locus_tag="POB34_003486"
     CDS             complement(10442..10705)
                     /gene="napD"
                     /locus_tag="POB34_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HDI5920372.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(10695..11189)
                     /gene="napF"
                     /locus_tag="POB34_003487"
     CDS             complement(10695..11189)
                     /gene="napF"
                     /locus_tag="POB34_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HDI5920373.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            11298..11462
                     /locus_tag="POB34_003488"
     CDS             11298..11462
                     /locus_tag="POB34_003488"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920374.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            11597..12085
                     /gene="eco"
                     /locus_tag="POB34_003489"
     CDS             11597..12085
                     /gene="eco"
                     /locus_tag="POB34_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HDI5920375.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
BASE COUNT         2938 a         3600 c         3097 g         2515 t
ORIGIN      
        1 gaactgttgt tcctgtgctt catctttaaa ctgcaacacg tcgatggtcg ccagcgctga
       61 gccggagatc atcagcatca gcacgcccaa taaaaacctc attgtgcggc ctccttactg
      121 tatttctccc acagcggctt gatctcttct tcccagacgc gaggattcag atcgcccgca
      181 tggcgatagc gaatgatgcc gttgccgtca ataaggaacg tttctggcgc gccatagaca
      241 ccgagatcca gccctaacat gccgtcgcca tcaaacaggc ttagcgcgta aggattgccc
      301 agctctttca gccagctgat tgccttctgg cgatcgtctt tatagttcat gccgaccacg
      361 cgaatgccct gcgctgaaag ctgattcaga tattgatgtt ccgcacggca ggtcggacac
      421 caggtcgccc agacgttaag cagtactggt ttgccctgag tcagcacatc cgcctgataa
      481 aactgccccg gattgtccag tgattcgaga cgaaacttcg gcacaggctt gccaatgagc
      541 gccgattcca gattggtcgg atcatcccct tcggcattac gcgccagctg ccacagcagc
      601 gccgcggcaa tcgccaggaa gataatcaac ggaattaaca atactttgcg cttcatacgg
      661 cctccggcgc agttttttgc ggactcacgc gcttacgata gcgaggatca aacagacaca
      721 gtagtccgcc caacgccatc atcagcccgc ccgcccaaat ccagcgaaca aatggtttgt
      781 agtaaagacg cacggcccac gcgccgtttt ccagctcttc accgagcgcg gcgtacaggt
      841 cacgcgtgat gccgccgtca attgccgctt cggtcatcat cgacccggca gtgttgtaat
      901 aacgtttttc cgcatacagc accgtttccg gtttaccctc tcgcgttacg ccaatagtcg
      961 ccacaccgcc gcgccagttc gggccagtca cctctttgac atcacggaag gtgaagcgat
     1021 attcatgaat atcgacgcta tcgccggact tcatgcgcac atcacgctca acgctataat
     1081 tctggctaaa ggcaatgcca acaattgtca ctgccagccc caggtgagcc gccaccatcc
     1141 cccaataact gaaggtggtt ttcgtgccgc gtgaaatacg tagcgcagct tccgcaattg
     1201 ccagcaccgc aatccagcag gccattgcca ggccgagcac cgtcatcgcc accactttgc
     1261 tttcgaacag ccacggcaac agcagagaca gcaccagcgt agagatgaag gcgataatca
     1321 ataaattgcg gattttacgt gggcgatcgc gcccccagcg caccagagga ccgacaccca
     1381 gcagtagcgc aaacggcacc atcagccagg taaacatggt gttgaagaac ggttcgccaa
     1441 tcgaaatact gcccagtccc agttgcttgt gcaccagcgg cagcagcgtc cccagcaaca
     1501 ccaccagcat cgcggcgacc agcaaaacgt tgttcgctaa cagcagagat tcccgcgacc
     1561 acagcgcatt gtttacgcgt gagcgaactt tgtgtccacg cgcggcaaac agcagcagcg
     1621 aaccgccaat caccagcacc ataaaggcga ggataaacat accgcgcgcc ggatcagacg
     1681 caaacgcgtg taccgatacc agcacgccgg aacgcacgag gaaagtcccc aacagacaca
     1741 acgagaaggc actgattgcc agcaataatg tccacgcttt gaagctggcg cgttgttcag
     1801 tgaccgccag tgagtgcatc agcgcagtcc ccaccagcca cggcataaac gaggcgtttt
     1861 ctaccggatc ccagaaccac cagccacccc agccgagttc gtaataggcc catgcggaac
     1921 cgagcacaat gccgagcgtc aggaaaatcc acgccgccag cgtccacggg cgagtaaaac
     1981 gcgcataagt gctgtccaga cgcccgctca gcaaagaagc aatggcaaaa gcaaacgcca
     2041 ccgagaaacc cacgtacccc atatagagca gaggcggatg gaagatcagc cccggatcct
     2101 gtaacagcgg gttaagatcg cgcccttcaa tcgggaagtt cggcagcgtg cgtgagaatg
     2161 ggttagaggt aaagagaatg aacagcaaaa agccgacgct gaccatcccc attatcgcca
     2221 gtacgcgggc cacaatatcc agcggaatac gctgactaaa aatcgccacc gcaaaggtcc
     2281 agccgctcat cagcagcacc cacagcagca gtgagccttc atgtgcgccc caggtcgccg
     2341 ccacgcgata ccacaccgga agctgggtat tggagttgct ggcaacatag gtgacggtaa
     2401 agtcgttgac cacgaaagca ttgaccagca ccagaaatgc gccagccaca gacataaaca
     2461 gcagccaggc aaacaagcgg gaagacgcca tcatgcgcgc atctccgcgc gccacgcccc
     2521 atagcggata cacggacagc agcagcgcaa tccctaacgc caggcacagc agcccgtttc
     2581 caatttccgg catcatgatg ctgggtcctt ataaacactc gccgggcgac ggtggttagc
     2641 ttccatcgct ttctcaactt ctggcggcgt gtagttttcg tcgtgtttcg ccagcacttc
     2701 ttttgcgagg atatgattgc ctttttccag ctcgccctgc accacaacgc cctgcccttc
     2761 acggaacaga tccggcaaaa tgccttcgta agagacatcc actgagcctt cagcgtcgta
     2821 aatggtgaag gtcactttca gcgaattggg atcgcgctgc acactacccg gcatcaccat
     2881 cccgccaacg cgcagacgct gaccgacttc cggcatttgc tgagtttcac gcttgccgta
     2941 gagaatttcc cccggcgtat aaaagagatc gatattcgag cgcagcgcat atagcaccag
     3001 accgatagtc agcgccagcc ctgccaacac ggcacaggca atccacaagc ggtttttacg
     3061 gcgaatattc atgcagcctc ctgctgttgc gcagcacgta aacgcgcctc acgcgcccgc
     3121 tgttgcgcca cgccacgcag aattgcgcga tgttgcatca ccgagtgcac gaccaaaacc
     3181 accagcggaa taacggtcat caccaccgcc agccagacaa aaaaggcgta accgcccatt
     3241 gcgaaaaatt cattccagga agcaaatgca ggggtcattt acggcctctt ttcagtatca
     3301 gttcactcac ccacggacgg cgtttttcca tcagcaaaat caaattacgc atccgcatca
     3361 gcgtcagcgt ggcagacagt agcaggaagc caaaaatcga ccagcgcagc ggcgaacgca
     3421 tcgccggatc gatactttgc tgcatccgcg ttgatccctg atgcagggtg ttccaccact
     3481 ccacggagta atgaataatc ggcagattca ccacgccaat cagcaccaga atacctgccg
     3541 cacggcccgc cagacggcgg tcgtcgaagg cgtgccacag ggcaatcaca cccacataca
     3601 aaaacagcag caccagttca gaagtcagac gtgcatccca tacccaccag gtgccccaca
     3661 tcggttttcc ccatgcagag ccggtaacca gggcaataaa ggtaaacacg gcaccaatgg
     3721 gggccatcgc cgccaccgcc aggttggcca ttttcatctg ccagacaagg ccaataaacg
     3781 ccgccaccgc cattgatgca taaatgccca tcgaccagat cgccgcaggc acatgcaggt
     3841 agataatgcg gtagctattt ccctgctgat aatcagccgg agcaaagccg aatccccaga
     3901 tccagccgac ggtaagcacg accacactgg caattgccag ccacggtata aaccagccac
     3961 agatttgata cagccgtggt gggatcgcca gttgatgcag tgttttccac atagtttcga
     4021 taccagactc gaacaaaaat cagtaatcca gcgttattga atgctgattc gtaacgctgc
     4081 cgccgtcgca aaaggactta atgtcgcggt gcctgccagc aacgcgccta aaattgccag
     4141 atacccgtca acgggcaaat gcatggaagc cgcgtccatc gcggcggtgg caaagatgag
     4201 taatgggata gtcagcggta acaccagtat gctgagcagc acaccaccgc gcttaagtcc
     4261 cactgtcaac gccacacccg gtgcaccgag aaagccaagc gtaggcgttc ccagcagcag
     4321 cgtcagcgcc atcacttgcc agccataaac atccattccc agtagcattg ctaccagtgg
     4381 cgaaaggatg agtaacggca gaccggttac catccagtgc gccatcacct tcgccagcac
     4441 aacggcgggc aagggtaacg gcaacaacat caattgttca agactgccgt cctgcaaatc
     4501 gtcacggaac agtcgttcca gcgccagcaa ggatgaaagc agcgcagcaa cccagataat
     4561 gcccggtgca atacgcgcca gcagttgcgg ctccggaccg atactgagcg gaaaaagggt
     4621 aattacaatc aggaagaacc acagcgggtt ggcgatttcg gcgctatggc gaaacgctac
     4681 acgcagctca agacggaaaa tgcgccagaa catcatgcgg ccctcgtttg cgtcagtgaa
     4741 atgcggcgaa ttttactttc agcaacgttg agcggctggt gggtagtcag aatcacaatc
     4801 cccccctgct ccgtatgctg cgccatacgc tgggtcagac gatcgacgcc gttaacgtca
     4861 atcgcggtaa aaggctcgtc gaggatccat aacgtggcac gggtcagcca cagacgcgct
     4921 aaagcgacgc ggcgttgttg cccggccgag agctgattta caggaatatc ttcgaatccg
     4981 gcaagcccgg cctgcgccag ggcttccaga cattgtgcgg tatcgccatc gcgatgataa
     5041 aagtgcagat tttctaacgc cgtcagccgg gttttgatcc ccggctgatg gcctatccat
     5101 aacaggtttt gatggtagct gtcgcgtacc tgatgcaagg gctgcccttg ccagagaacc
     5161 tcgcctgcgt cagggcgaga caaccccgtc agcaaacgga gaagcgttgt cttccccgcg
     5221 ccgttgctac cggtgatttg tacccactct cctgcgttca gcgtaaatga caagccacta
     5281 aataaggttc gttcatcccg ctcacaaagt aactctctgg cttcaagcat acccacgcaa
     5341 taaccctgtt aaaaacctgg ctcgacttca cgcatatcgg gcagcttgtg cgctatccct
     5401 ttatggcaat caatacaggt ttgcccatct ttcaccgcct ggtcatgcat cttcgcggca
     5461 accgatttct gggcggttgt atccatatac tcgaagttgt gacagttacg gcactcctgc
     5521 gagttattgt ccttcatgcg ccgccactca ttctgtgcca tcgtcagacg atgagcttca
     5581 aatttctgcg gcgtgtcaat aacgccaaaa attttaccat acagctcttt acttgctttg
     5641 agcttgcgta tcatcttcgg cacaaactcg tgcggaacgt gacaatccgg acaggtcgca
     5701 cggacgccgc tacggttgtt gtagtgcacg gaatccatgt attcctgata caccgtgttg
     5761 cgcatttcgt ggcagctaat gcagaactct tcggtattgg ctttttccat cccggtgtta
     5821 aagccacccc agaagacgat gccgccaaca aaaccgatca acagcagcgt ccccagcgcc
     5881 agacggctgg gggtacgcca ccatttccac aggcgcttaa tcagaccagg cttacggtca
     5941 gaatttccca taataacctc ttatttcccg taaccttttg atggggtaaa ggtattcccc
     6001 acgattggcg cggtatcggc ctgcggtacg tgacattgca gacagaaata acgacgcgga
     6061 gccacttccg cgcccacttt gccgtcgctg tccataaagt gagtaggact gatacgcggc
     6121 gcgccagtgg tgcgatagct ttcgacaccg tggcactgca agcagcggtt ggtattggtc
     6181 gttacctgat aaccttcaac gctatgcggg atcattggcg gctgattcac atagttcagc
     6241 ggcatccgtt cctgctcttt tggcatccga atggcccctt cctgtgtccc ggatacttcc
     6301 ggcgactggc taaaatcgac gccattagcc gcccaaaccg cgccgcttac caccaaggcc
     6361 agcatcgccg tccattgaca cagcgctttc ttcaggtcat ggcttttcat gatttcgctc
     6421 ccgaactcca tcgtgtagtt attgtaaata catcctcaga acagacatcc acgcagcgac
     6481 cgcacgtcat gcaatcgcgg ctggtgacct gcaccgggct ttgttcatcc agcaccgggg
     6541 cacgtagcac atgcggttcc gggcaaacat gaaaacaatc catacagcgg ttacatttct
     6601 gccgatcggt ggccgcaacg gtaatcacac ctttgctacc cagcacgcca tacagcgcgc
     6661 caaccggaca aatgtgcccg caccagccgt gttcaacgac cagtagatca aataaaaaca
     6721 gcgcgagaat aagcagcgcg ccgctgccga agcccattac caggctacgc cccatcagag
     6781 aaacggggtt gatccattcc caaatgagcg tgccagttaa ggctgatccc accagaatga
     6841 ccaccaacag cacgtagcga atgtggcgag ggatcgtcgc agactgattg aggtcaaagc
     6901 gcctgcgtaa ccagttcgct aagtcggtaa tcgggttcag cgggcagacc cagctgcaaa
     6961 ataatcgctt tcccgccagg gcgtagagca cggtgataat caccgcgccg gtcaacgcca
     7021 ccgtggcagg cagatgacca ctggcaagac tttgcagcgt catcagcgga tcggtcagcg
     7081 gaacggtgtc gaataacagg ctactgctgt agttgccgtg caagatccac acaccaaacc
     7141 atggaccgct caaaaacatc cccagcacga agaactgaca aaggcgacgt aacaccagcc
     7201 aacggtgact gcgccaccag ccttttttct ccagcgcctc gcgcccggcg tcacgtttac
     7261 gatttgccat tgttcccctc cagccagccg aagcggtaat ggtgacctaa ctcccctttc
     7321 gccagtgaca gcggtaacac cttgattgcc ggttgttcca gcacgcatac tttttcgcac
     7381 ttaccgcaac cggtacaggc gtcgctgtga accgtcggca gaaagcgggc gtgcttaccg
     7441 gtacgcgtgt tgcgctccag ctccagggtg atggcctcat caattttcgg gcattcgcga
     7501 taacaaacat cgcagcgcag cccctgaaag ttgagacagt tttcctggtc caccagtacc
     7561 gccagcccca tccgcgcgtc gtcgatcgat tcaatctcac gatccagcgc accgcttggg
     7621 cacactttgg cgcacggaat gtcctcacac atttcgcaag gaatatcccg tgcgacaaaa
     7681 tatggcgtgc ccgccgacag accagaggcc agcgtcgcca gttttaaggt gtcgtaaggg
     7741 caagcctgaa cacactgacc gcaacgcaca caggcactgg caaaggcgtt ctcgtttatg
     7801 gctccgggcg ggcgcaaccg cacgccagat gcgcgtgcgg tttgctgttg taaccccagc
     7861 gccacaccca cggcagccag cccgcctgct gtgcgaacaa catcgcgcag aaagcggcgg
     7921 cgaccatttt gaggtttcgc tgaccgggac ataatggctt acaccttctc cagtttgacc
     7981 gcgcacttct tgaagtccgt ctctttcgag agcggatcgg tcgcatccag cgtcagtttg
     8041 ttaaccagct gtgcggcgtc gaagaacggc atgtacacca ggccctgtgg cggacggtta
     8101 cgaccgcgcg tttcaacaat cgagatcact tcgccacggc gagaaaccac tttcactttg
     8161 tcgccacggc gcagatcgcg cgctttcgca tccagcgggt gaataaacaa gaccgcttcc
     8221 gggaaggcgc ggtgcagttc cggcacacgg cgagtcatac tgccggtgtg ccagtgctcc
     8281 aggacgcgtc cggtagagag ccacaggtcg tactcttcat ccggtgcttc cgccgccggt
     8341 tcgaacggca gcgcgaagat caccgctttg ccatccggtt taccgtagaa cttgtagcct
     8401 tcgcccgctt tcacgtacgg gtcgttacct tcgctgtaac gccactgcgt ttctttaccg
     8461 ttcaccaccg gccagcgcag accgcgcgct ttgtggtagt catcgaacgg tgccagatcg
     8521 tgaccgtgac cgcgaccaaa ccatgcgtac tcttcgaaca gccctttttg cagatagaag
     8581 cccagctcgc gggattcatc gttcagctga tcttccgcca gttcggatac cgggaatttg
     8641 ctcacttcgg gggtggcata cagaacttcg tacagcgttt tgccacgcag ttccggtttc
     8701 ttcgccagca gctcttccgg ccatacttct tcagttttga agcggcggga gaactggact
     8761 aactgccaga gatccgattt cgcttcgccc ggtgcctgta cctgctgacg ccagaactga
     8821 gtacggcgtt cggcgttacc gtaagcgccc tctttctcta cccacattgc ggtcggcagg
     8881 atcaagtcgg cggccagcgc actgactgtc ggatacggat cggagacgat gatgaagttg
     8941 cgcggatcgc gccagcccgg catacgctct tcattgatgt tcggcccggc ctgcatgttg
     9001 ttggtacaca tggtccagta aacattcagc ttgccgtctt tcagcgcacg gtcttgtgct
     9061 accgcatgca gaccgatttt cgccggaatg gtgccgctcg ggatattcca cttcttctcg
     9121 cagatatcac gatgtttctc gttagtcacc accatgtccg caggcagacg gtgagcaaag
     9181 gtgcccactt cacgcgcagt accacacgcg gaaggctgcc cggtcaggga gaacggaccg
     9241 caacccggct gggaaatttt gccggtcagc aggtgcaggt tgtagaccag gttgttggcc
     9301 cacacgccac gagtatgctg gttgaagccc atcgtccagt aggagatgac tttcttgttc
     9361 ggatcggcat acagctgcgc cagttgttct aactggtctt tcggcacgcc ggtcatttcg
     9421 gcagtttttt ccagcgtata ctcggcaacg aaggctttgt aatcttcaaa gctcatcggt
     9481 tcggaggcgt cagaacccgg attcttcgct gctttttcca gcggatgggt cggacgtaaa
     9541 ccgtagccga tgtccgtcgc ccctttgcgc aggttaacgt gcttgctgaa gaagtcctga
     9601 tttatcgcat tgttttgaat gatatagttg gcgatgtagt tcaggatcac caggtcagat
     9661 tgcggcgtaa agatgatgcc gttatccgcc agctcgaagc tacgatgctg gtaggtagaa
     9721 agcaccgcca cggtgacgtt ctggttagag agacgacggt tagtgatgcg tgaccagagg
     9781 atcgggtgca tctccgccat gttagcgccc cacagcacaa acgcgtcagc ctgctcgatg
     9841 tcgtcatagc agcccatcgg ctcatccata ccaaaggtac gcataaagcc aactactgcc
     9901 gacgccatac agtgacgtgc gttcgggtcg atgttgttcg aacggaagcc cgctttgaac
     9961 agcttggacg cggcataacc ttcccagata gtccactgac cagaaccgaa cataccgatc
    10021 gattccggtc ctttttcttt cagggcggtt ttgaatttct cttccatcac atcgaaggcc
    10081 tgatcccagg tgattggggt aaattcgcct tctttgtcat atttaccgtt tttcatacgc
    10141 agcagcggct gcgtcaaacg gtctttaccg tacatgatct tgggcaggaa atagccctta
    10201 atgcagttca ggccacggtt aaccggtgcg tccgggtcgc cctgacaggc caccacacgc
    10261 ccctgctgcg ttccgaccag aacgccgcaa ccagtaccgc agaaacggca cggcgcttta
    10321 tcccatttaa tggcttcctg ctgaccaaca acggcgcggg caacgcccgg cacgctgaga
    10381 ccggcagccg ccgcagcggc cgcaacggcg ttagctttca taaagctacg acgactgagt
    10441 ttcatggtgt ttcctcacct tgctcttcct gctggtgata aaccagcgac accgccagca
    10501 cgccctctac gttgcgtact gactcaatgg tttggatcag cgtttcgctg tcttctgctt
    10561 ccaccaccac aatcaactga ccgctcggcg cgtcgctgac agcaacttca cagccgggaa
    10621 aggcgttcag ttgggtgctg atgtctgaaa ttcgttcgct tttggcctgc acgaccaggc
    10681 tgcaaacttg ccagttagtg tgcatggaga tactccgcag ttatggctga taccggacag
    10741 ctggctgcac atgctccaca tccgttgcaa agttggctat taagttgcgg ctggtagatt
    10801 ccggagagcg tcgggcgaaa gataattgcc attggctcac agctatcctg acagcggcgg
    10861 cattcaactg actgatacgc caggcacgcg tccccaatgg tgaactgcaa atcccaggcc
    10921 ctggtgtggc gcggagaaaa tagcgattcg gggcaagcct gcgcgcaggc gtaacagaag
    10981 ctgcactcgt tatttttgaa attaacgctc ggatagccgc ccgcgccgcg ttgcagaatg
    11041 ttgttttcac aagcattaat acaggcgtcg caacgggtgc aatgggtcag aaaatgagat
    11101 tcatcaccgg accagggcgg acggataccg ttactggctt tgcgccagcg accagtgagt
    11161 atgccccgac gggatgcatc aatcttcaca ttgaccttcc atcattaacg cgctctgaaa
    11221 attgagagcg accaaataaa ccgcataatt aataagccat ttttatagcc gctaagatat
    11281 taaaggatgt gtcaaagatg cataccccga tcggggtaaa acctgtagca ggatcaaaag
    11341 agtggcggga agcgtggcaa aaacgggctt ttgctcacat ttcaaatggt tataaacata
    11401 tttatatagc gattaattca ccagagatat ttctgctggt ttgctccctc atcagaattt
    11461 aacactaaaa gagcaggtaa aattgtctga atgttcttta agttattcat aaagcaaatt
    11521 aataaatctg atgaatatgt taaccttcag cgacatcatc ggtgaaaacc tataaatgaa
    11581 gaaggaaagc aaaaaaatga agaccattct acctgcagta ttgtttgccg ctttcgctac
    11641 cacttccgcc tgggcggcag aaagcgtcca gccactggaa aaaatcgcgc cttatccaca
    11701 agctgaaaaa gggatgaagc gtcaggtgat tcagttaacc ccgcaagaag atgaatctac
    11761 cctgaaagta gaactgttaa tcggtcagac gctggaagtc gattgcaatt tgcatcgtct
    11821 cggcgggaag ctggaaagca aaacgctgga aggctggggc tatgattatt atgtctttga
    11881 taaagttagc tcgccggttt ccacgatgat ggcctgcccg gatggcaaga aagagaagaa
    11941 atttgtcacc gcgtatctgg gcgatgctgg aatgctgcgt tacaacagca agctgccgat
    12001 cgtggtgtat acgccagaca atgtagatgt gaagtaccgc gtctggaagg cggaagagaa
    12061 aattgacaac gcggtagttc gctaaactgc cgtgaagtgc ggcaccccgt aggtcagaca
    12121 aggcggtcac gccgcatccg acatccaacg
//
LOCUS       DAMZET010000126        12096 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.126, whole genome shotgun sequence.
ACCESSION   DAMZET010000126 DAMZET010000000
VERSION     DAMZET010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.126"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(129..1490)
                     /gene="allB"
                     /locus_tag="POB34_003490"
     CDS             complement(129..1490)
                     /gene="allB"
                     /locus_tag="POB34_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HDI5920376.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(1550..3004)
                     /locus_tag="POB34_003491"
                     /pseudo
     CDS             complement(1550..3004)
                     /locus_tag="POB34_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
     gene            complement(3173..4051)
                     /gene="glxR"
                     /locus_tag="POB34_003492"
     CDS             complement(3173..4051)
                     /gene="glxR"
                     /locus_tag="POB34_003492"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDI5920377.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(4151..4927)
                     /gene="hyi"
                     /locus_tag="POB34_003493"
     CDS             complement(4151..4927)
                     /gene="hyi"
                     /locus_tag="POB34_003493"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HDI5920378.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(4940..6721)
                     /gene="gcl"
                     /locus_tag="POB34_003494"
     CDS             complement(4940..6721)
                     /gene="gcl"
                     /locus_tag="POB34_003494"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HDI5920379.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(6811..7626)
                     /gene="allR"
                     /locus_tag="POB34_003495"
     CDS             complement(6811..7626)
                     /gene="allR"
                     /locus_tag="POB34_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HDI5920380.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(7704..8186)
                     /gene="allA"
                     /locus_tag="POB34_003496"
     CDS             complement(7704..8186)
                     /gene="allA"
                     /locus_tag="POB34_003496"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HDI5920381.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWLRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            8416..9342
                     /gene="allS"
                     /locus_tag="POB34_003497"
     CDS             8416..9342
                     /gene="allS"
                     /locus_tag="POB34_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HDI5920382.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            9411..10505
                     /gene="mnmH"
                     /locus_tag="POB34_003498"
     CDS             9411..10505
                     /gene="mnmH"
                     /locus_tag="POB34_003498"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDI5920383.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEHGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEVGINYPLVEGGYKVLRQTAIQATIELSQKPIVLIGGCTGCGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            complement(11217..>12096)
                     /locus_tag="POB34_003499"
     CDS             complement(11217..>12096)
                     /locus_tag="POB34_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5920384.1"
                     /translation="HIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRP
                     DHNPIVAGNWPPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWES
                     LRPNFYFIFPEGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQ
                     VLEQVSRALEVMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWC
                     EFAMLGFVSGLVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWL
                     GARLVKGKALFRQFAG"
BASE COUNT         3166 a         3222 c         2836 g         2872 t
ORIGIN      
        1 gttgcaaaaa ataaagaaaa accactgaaa tcctgataac aggctttcgc ggctgactgc
       61 aaaattgaac ataaacacac cttaaccgga gaatgcccgc cgcaaggacg ggcattgcag
      121 gggccagatt actgctgatg tttaaggata aattgacctt tcggcgcaac agggaagccc
      181 tgttcaatgt cgtaaatcac atcaccacgt aagatggttt tcgtgatacg cgcgccaatg
      241 gtacggccaa catacgggct gactttgtgg cgatattcca gatcgtcatt ggtaagaaca
      301 tagctgctat tcggctgaat gaagacgaag tcggcatctt ttcctggggc gatacggcct
      361 ttttgctgca gaccgaaaat atctgctgcg ttagtcgcca ttaatttgcc gaacattggc
      421 agagacattc cgcgtttctg taccgcttca tcgaacatca cgtccataca gttttgcaga
      481 ccggcaatac cgccccatgc ttccatgatg ttgccggctt tcatttccgg agggcatggt
      541 gagtggtcgg aaaccaggca gtcgatttca ccgttaaaca gtttttccca catgcctttc
      601 tggttttcca gatcgcggat cggcggtgaa cacttcgcca gagtgccgat ttcttcgaac
      661 tgatcggtat ccagtacaaa gtaatgcggg caggattcac aggtaacatc ctgaccttcc
      721 tgacgtgcac gagtcacttc ttcaacacct tccgggctgc tgatatggca aacgtgcaga
      781 cggcaaccgg caactttcgc cagatacagc acgcggcgaa tcgcttccac ttcggtaaat
      841 accggacgcg aagccacata gtcatgggca gttacgcgac cttcacgttt cgcttcttcg
      901 cccagtgcgt cacagatcag cgcgttttcg cagtgcacca acaccggctg ccccagttcg
      961 cccagcttct gcgcaccttt gaagaactgc cagtcgttta catcacggaa gtcgttgtcg
     1021 ataccgcgat cgccacaggt cgcaacgaag catttgaagc cgacaacgcc cacttcatcc
     1081 aactcatgca gacgatcaat gttgtaagac accaggccac cgagttgtgc cgcatcgata
     1141 gtcagcttgc ctttagcggc atcgaacttc agttcaattg aagcacggtc aaccgttgca
     1201 ggcagctggt tgagcggcat ttcgatcatg gtggtgatac caccttttgc cgctgcgcga
     1261 gtaccggttt cataaccttc ccagtggcta cgacccggtt cagaaatatg ggtgtgcgca
     1321 tcaaccatgc ccggcgaaac caccagacca gacgcatcca taacttcttt tgcatcgccc
     1381 agatcctgac cgatagcagc aatttttccg cctttaacgg cgatatctac aacgcgagct
     1441 tcgttttcta aaataacggt gccgttttta atgattaaat caaaagacat aacaaactcc
     1501 ttgtaaaata cagattggtt tttaactcaa tgttcaatat cgggattaat taacctatgg
     1561 ttttttgctc tcctgttttt tctgctgttg tacgtttctt taataaggcg taggccgcaa
     1621 aggcgacgat gacgccgaca aaccatgaaa cacgcgataa cggttccata aagggaataa
     1681 acttaccgcc aagagataaa ataacggcca ccagagttac tgaaaacgca gtgaggttaa
     1741 aaccgttatc gtaatattta taatcgccag gtgcggtata cagttcatca agattaattt
     1801 tcccacgcat caccacaaaa taatgcgcca tcatgacacc aattaccgga ccaagcattc
     1861 cgccgataat atcgaggaac agataaatac tgtcctgatt ttccattaat ttccacgggc
     1921 agatcagcag gctgataata ctggcaatca gtacgccgtt tttataagtc agtttcgtcg
     1981 gtgcaatagc ggcaatctga taaccggcag gaataatatt accggtggcg ttagtggaga
     2041 tagtcgtcat cagaataacc agtaccgcaa agaacgaggc gaacaagctg tcccaacgct
     2101 gaacaatatc cagcacgttc taggtatcag cgccgtaatg aatactggct ccggcaataa
     2161 tacatacccc cgcgaccgca aacagaatat aggccacaac taaacccagc gtttgcccca
     2221 gcgcttgctc acgaaacgag tgggcgtttt gcgtaaagtc ggatgcgctc accgccggtg
     2281 ccgcccagac cgcaactacc gcgtttatca ccaccaggaa caggaagcca ctgttttctg
     2341 ctttctgaat accgctcgga atgtagtcaa agattggacc gagcccgacc agcgaaatcg
     2401 cccaaatcgc cataccgccg aaaacgatat agatgcacgg gttaagaatg gcagtgaatt
     2461 tatttaaaac tttgccaccg ccaaaaccta taccaacgtt gaccagccag aagagtaaga
     2521 aagtaattaa gcccggtaga gaaagcccta acagcgtgaa atcaccaccg agagttaaaa
     2581 atcccggcca gattttgcca atcagaatca agcaggccag tgaccccgcg taacattgca
     2641 ggccaaacca cataatggca gcaataccgc ctcttaataa tccgggaaac agtgcgccac
     2701 gtacgccgta agaagcacgc aggatcatgg caaacggtac gccgtattta ctgcccgcag
     2761 cgccgtttaa taccattacc gcggcaatga aaaaggcgct gaggataatt gccagcataa
     2821 tactaaaggt agacaagccg agaataaaaa agccgccgac catcacataa ttgggaacgt
     2881 tatgaaccga acccatccat aaggtaaaat agttaaatgt tttccaggtc cgctggcttt
     2941 gcgttttcgg caatagatct tcgctatagc cgcgttgctg gaatagtttt ctctgatgtt
     3001 ccataaatcc ctcttaattt cacgtatcga ataacgttgt tttaatctag ctatgaggaa
     3061 caaataaaat aaaaatatta tttttaatac tgaagcaaca tctttggctg ctttcttttt
     3121 taatattgct ggccgattca ttttctgata tcggccattt ttattgcggg tatcaggcca
     3181 gtttatggtt agccattaat tccagcgcct gcactaacgc agagtgatcc aactggctgc
     3241 caccgtttgc cgcacaggta ttaaataact cctggcaggt cgcagtgttt ggcaggttca
     3301 gcgcaagtgc tttcgcactt tgcagtgcca ggttgagatc tttctggtgc agagcgattt
     3361 tgaagcccgg attaaaggtg cgtttaatca tacgctcgcc gtgaacttcc agaatacgtg
     3421 aggaagcaaa gccgcccatc agcgcctggc gcacacgtac cgggtccgca ccggcttttg
     3481 aagcaaatag cagggcttca gaaaccgctt caatattgag cgccacgata atctgatttg
     3541 ccactttgca ggtttgaccg tcgccgttac cgcccacgag ggtgatattt ttaccgagca
     3601 gttcaaaaag cggtttaaca cgttcaaata ccgcttcatc accgccaacc ataatcgaca
     3661 acgtcccttc acgcgcaccg atttcaccgc cggagactgg cgcatcgaga taatcgccgc
     3721 ccagttcatt cacctgacga gcgaaacgct tagtttcaat cggggaaatg gagctcatat
     3781 caacaatggt tttgcccttc agcgaggctt tggtacaacc attttcaccg aacagaactt
     3841 cttcaacctg tggtgtatct ggcaccataa taaaaatgat gtccgatgct tccgttacct
     3901 ggcgcgcagt ttcaacactg acggcaccca gtgacagtaa ttcatcagca accggaccaa
     3961 tggttgtgac atgcagttga tgaccggcac gcgccagatt aatggccatc ggtgtaccca
     4021 taatgcctaa gccaataaat cccagtttca taattaacct cttttaaatt tcgcttttcc
     4081 tgaattcaga caacacgatc ccctgccaac ttagcggcct aaaaaagcat tgcctgaaca
     4141 gcgttacgtt ttaacggtac ggatccatcc agcgtaaacc ggcttccgtg gtggtttggg
     4201 gtttatattc acacccaacc caaccgttgt agtcagaatt ttcgattacc ttaaagagat
     4261 aatcataatt aatttctccg gtccccggtt cgccgcgatg cggattatca gcaatttgca
     4321 ggtggccaat tttatcggcc cactgagtca tggtgttggt taattcacct tccatccgct
     4381 gcatgtgata aatgtcatac tgaattttca aattgcagca accaacatca tcaatcaatt
     4441 tcagcgcctg ccgagttccg gtgagatgga aaccaggaat atcaaaatgg ttaataggtt
     4501 caatcagtaa taaaatatct tctttcatca gcatattcgc ggcataacgc aggttttcta
     4561 caagcgttgc gtgaatctgt tcactgctga aaccagccgg cgttttaccg accagacagt
     4621 taattttttt attccccagc gcacgggcat aacgaatcgc tgctgctacg ccatcccgaa
     4681 actcttcttc acggccagga atacaggcaa taccgcgctc ccccgccgcc cagtcacccg
     4741 ccggtagatt gtgcagcgta tgttcgagtt tattactcgc cagcacctgt tttaattctt
     4801 caatgtcgta gtcataagga aacataaatt caacgccgcg aaaaccacac tgcgcagctt
     4861 tctcaaaacg ggcgagaaaa tcatattctc caaataacat cgataaatta gcagagaaac
     4921 gtaacattat ttatctccct tattcatagt gcatgaagca ggtttcagtc ggtgcgtccg
     4981 ctgcgttatc ggcgatatct tcaaattcca tgacgttatc cagttcgctg cccatcgaaa
     5041 tattggtcac acgctcgaga ataacttcca cgactaccgg tacccgatat tgcgccatta
     5101 aggctttcgc ctgttcaaag gctggcgcaa tatcttccgg tttgaagacg cgaatcgctt
     5161 tacaacctaa accttccgct acttttacgt ggtcgacgcc gtaaccgttc acttcgctgg
     5221 agttgatatt ctcgaaagcg agttgcacgc agtagtccat atcaaacgcg cgctgcgact
     5281 ggcgaatcag ccccagataa gcgttgttga ccagcacatg gatgtacgga atgttgaact
     5341 gcgcgccaac agctaactct tcaatcagga actggaagtc aaagtcgcca gaaatcgcca
     5401 ccacattgcg tttcggatca gcggcacaaa cccctagcgc agccggaatc gtccagccta
     5461 acggaccagc ctgaccacag ttgatccagt ggcggtcttt aaagacatgc agcatttgtg
     5521 ccgcagcgat ttgtgacaga ccaatggtgg tgacataaca aacatcgcga ccaaaggctt
     5581 tgttcatctc ttcatacacg cgctgcggtt tcaccggcac gttgtcgaag tgggttttgc
     5641 gcagcaaagt gcgtttacgc tgctggcagt cggcgaccca ttctttacga cacggcagac
     5701 gacccgcttt ttgcatctcc tgcgccactt caaccagcag tgtcagcgcc gctttagcat
     5761 cagagacaat accgagatcc ggacacagca cgcgaccaat ttgcgtcggc tcaatatcaa
     5821 tatgaacgat tttgcgccct tcggtgtatt tctctaccga gccggtatga cggttagcaa
     5881 aacggttacc gataccaaac accatgtcag acgccagcag cgttgcgtta ccgtaacgat
     5941 gcgcggtttg cagacccacc atcccggcca tcagttcatg atcgtccggg atacagcccc
     6001 agcccattag cgttgggatc accggaacgc tggtcagttc agcaaactgt tgtaacagtg
     6061 cagctgcgtc agcattaatt accccgcccc cggcaacaat caccggacgt tcggcctgga
     6121 ttaacatttc tacagctttt tcgatctgca tacggctggc agcaggtttg tagaccggca
     6181 gcggttcgta catgtcagga tcaaactcga tttccgcaac ctgaacgtcg aacggtaaat
     6241 ccaccagtac cggacccgga cgaccagaac gcatcaggtg aaatgcctgt tgcagcacgc
     6301 gaggcaccag cgccgcttca cgaactgtaa ccgccatttt gctgaccggt ttagcaattg
     6361 cttcaatatc tacggcctga aaatcttctt tatgcagacg ggcgcgcggt gcctggccgg
     6421 taatgcacag aataggaatg gaatcagcag aagcggaata gagcgcggtg atcatgtccg
     6481 tgcccgcagg accggaagtc cccagacata cgccgatatt ccctgccgtt gcgcgggtat
     6541 aaccttccgc catgtgcgaa gcaccttcca catgacgcgc cagaatgtga cgaataccgc
     6601 cgtgcttacg catcgctgag tagaacggat tgattgcagc tcccggaacg ccgaaggcgg
     6661 tggtgatacc ttctttctcc agcacataca ttgccgcgtc aacggctctc atttttgcca
     6721 ttttattcct acctctatta attggaaaaa ttttccaact ttcaaatttt gtcaaaaaaa
     6781 cccgctccgc agagcgggat gcgacagaca ctatggatgt gctttcagtc ccaacgccgt
     6841 gctgatatcg cgggcggtgt ctctgaccag ctcaccctga ctgacaaaac gatcttctgt
     6901 cagccttgat gaaggcccgg agatggagat agcggcaaca acactaccta catcatcgta
     6961 aattgctgaa gctatgcaat tcagacctac aacatgctct tctttatcta cggtatagcc
     7021 cagttcacgc gcttgctcca ggtccttcag taaggtgggc atatccacaa gcgtagttgg
     7081 cgtaaactgc tgcaaaccgg tttgcagaat gatgctcatc aactcctctt ccgccagcgg
     7141 ataaagcagc gctttgcccg caccggaagc atgcagtggc agacgactgc ccagtggcgc
     7201 acacatcctg accatcgatt tacactctag ctgaccaatt aatactgctt cattgccgtt
     7261 acggatcgcg acattgaccg tttcgccgga aagtaacatc aggcggcgca taaacggccc
     7321 ggcgacggag aggacatcgc ggttatggat gtacgccgca ccgacgttaa agacacctaa
     7381 tcctatatgc caccagccta actgactgtc ctgatagaca aaatccgctg cctgtaaaac
     7441 cttcagcaag cgaaaggtcg tggagagcgg caaatccaga ttgagagaaa tatcgctaac
     7501 cgacgaactt cccccacttt tttccagata ttgcagaatc gcaattcccc gctctaacgc
     7561 ctgtgcgccc ttctgtgcca caggctccgc ctgtcctggc ctgccgcgcc gtctaacttc
     7621 cgtcatacct acgttaactc cgacctacaa gtgagcataa acaatgaaca attaatttat
     7681 cttatgcaag tcggttgcag gcgtcacgca aaacagagtt cctgttcagg aatactttca
     7741 acatcacagt tgtcactgcc gccgcgatcg atggtcagaa aatcggtgac gcgcagccag
     7801 gcgaaaagtg ggtgatgcca gacgttacga tggtaattca ccccctgttc accgttggtg
     7861 ataaacgccc gcagggttga caggtctggt ttgtcgtcac ctaacgccac gaccaccaca
     7921 aacacttcac ctttcatcgg gataaaggcc tgagtaccca gcggatgacg ttcgagttcg
     7981 tgaatggtca gcggtagatt cgccggttgc gcgcggttaa tgctgataag cgtacggtct
     8041 tgctcaagaa tctcaaccag cgccaaatcg tggtaacgct ccaccaggcc attgttaata
     8101 tggaaaaaat cccgttgctg cgtttcgatt acgtcgccat aagcactaaa ggcttcctga
     8161 cttaacggta atacctgaag tttcatcttt ttgtccttgt aacataattc cgttcaacca
     8221 ccgccgaccg gtcagcttgg tttcaataac aggcaatcta aaaccttaag agaacaactt
     8281 caatacaaaa gttggaatat ttttccaaaa ctagatttag tccacaaatt gaacatggtt
     8341 atcatgtaaa aatactgata aatcagagga taagaaaatc acgcaacttg cggatttaaa
     8401 aggggatacc tgaagatgtt cgatccagaa accttgcgga ctttcattgc ggttgctgaa
     8461 acaggaagtt tttcaaaagc ggcagaacga ttatgtaaaa ccacagcgac gatcagttat
     8521 cgcattaaac ttctggaaga gaataccgga gtagcgctgt ttttccgcac gactcgcagc
     8581 gtgacgttga cagcggctgg cgagcatcta ctttcccagg ccagagactg gctgagctgg
     8641 ctggaaagta tgccaagcga gctgcaacag gtgaatgatg gcgtggaacg ccaggtgaat
     8701 attgtcatca acaacctgct ctacaacccc caggccgtcg cccagttgct ggcgtggctg
     8761 aatgaacgtt acccctttac ccagtttcac atctcccgac aaatctatat gggcgtctgg
     8821 gactcgctat tgtacgaagg tttttcactg gctatcggcg tcacgggaac cgaggcgctg
     8881 gcaaatacct ttagtcttga tcccttagga tcggtgcaat ggcgctttgt catggcggcg
     8941 gatcatccgc tggcgaacgt tgaagagccg ctaacagaag cgcagttgcg gcgctttccg
     9001 gcggtcaata ttgaagacag cgcccgcacc ttaaccaaac gcgtcgcctg gcgattgcca
     9061 gggcaaaaag agattattgt tcctgatatg gaaacgaaaa tcgccgccca tctggcgggc
     9121 gttggcattg gttttttgcc aaaatcactt tgccagtcaa tgatcgataa tcaacaactg
     9181 gtcagccggg taatcccaac gatgcgccct ccttcgccat tgagtctggc atggcgcaaa
     9241 tttggcagcg gcaaagcggt agaagatatt gtgaccttgt ttacccaacg caggccggaa
     9301 atcagcggat ttttagaaat tttcggcaac ccacgcagtt aaaaatcaag tttccttgcg
     9361 cgttggcgcg taaaatagcg cgcaagctga tttgaggttc acttttaact atgcaagaga
     9421 gacacacgga acaggactat cgtgccctgc tgattgctga tacgcccatt attgatgttc
     9481 gcgcccctat cgagtttgag cacggcgcaa tgcccgccgc tatcaatctg ccgttaatga
     9541 ataacgatga acgcgccgcc gttggcacct gctataaaca gcaaggctca gacgcagcgc
     9601 tggcgctggg acataaactg gtggcgggtg aaattcgtca gcagcgcatg gacgcctggc
     9661 gggcagcgtg cctgcaaaat ccgcaaggta ttctctgctg cgcccgtggc ggtcaacgtt
     9721 cacatattgt gcaacgctgg ttgcatgaag tggggattaa ttatccgctg gtggaaggcg
     9781 gttataaggt actgcgccag actgcgattc aggcgaccat tgaactgtca caaaaaccga
     9841 tagtgctgat tggcggttgt accggctgcg gcaaaacgct gttagtgcag caacagccga
     9901 acggtgttga tctggaaggg ttggcgcgtc atcgcggttc ggcgtttggt cgcaccttac
     9961 aaccacaact tagccaggcg agttttgaaa acctgctggc tgccgaaatg ctaaaaaccg
    10021 acgcccgtca gaatttgcgc ctgtgggtgc tggaagacga aagccggatg atcggttcga
    10081 atcacctgcc ggaatgtctg cgcgagcgaa tgactcaggc ggcgattgcg gtggtagaag
    10141 atccgtttga gatccgtctt gaacgcctga acgaagagta tttcttgcgt atgcatcatg
    10201 attttaccca cgcttatggc gacgaacagg gctggcagga atattgcgaa tacctgcatc
    10261 acggactttc ggcgattaag cgtcggctgg ggctacagcg atataacgaa ctggctgcaa
    10321 ggctggatgc agcactgaca acgcaactca ccaccggcag caccgacggt catctggcct
    10381 ggctggtgcc gttacttgaa gaatattacg acccgatgta tcgctatcag cttgagaaaa
    10441 aagcggaaaa agttgtcttt cgcggtgagt gggcggaagt ggcggaatgg gttaaggcgc
    10501 agtgatgggc ggtattctga aacggcgatt gtcgtgagaa cagaatggtt gcaccagaat
    10561 acagatataa aaaaaccgac atgacagtcg gtttttttat tttttccgcc caagacttgg
    10621 tggttccggg cagaaatgaa acagtcctca tctagcaagt gaattataag tcaggatgcc
    10681 tacatttcaa acagctatgt tggcgataca atcccaacgc tagtagtgat actgcctcaa
    10741 gggattaccg cagtgcggaa aagtactttt aagtcctggt caggatacag gtggtgctga
    10801 ttctggtgct ctaatttgga gagaggcagt cctcatctag cacatgaaga aaatcatagt
    10861 tacaggcaac gtaagggtaa gctgtgattg tctttctccc ttaagggagc aacggatgaa
    10921 aacttttctc gataaagttc tcatcgttct taaggcgcta atcgcgctgc tggaactgat
    10981 ccgtcagttt attgaataac tgaggaaacg tagctaaggc cgaaggttta ccaccttcgg
    11041 cctttaaaaa aaccgacatg acagttggtt tcatatttat tgccaaaatg cacactgttc
    11101 atgccagatg cggcgtaaac gccttatccg gcctacacaa tcttgaaaaa tcaatatatt
    11161 gcacgctctt cgtaggcctg ataagcgtag cgcatcaggc aatctaacgc ctgtcatcac
    11221 cccgcaaact gcctgaacag cgccttaccc ttaaccagtc gcgcacccag ccagccgccg
    11281 aaaagcgaca gcagcagcgc accgctgcac ggcagaacaa tccacaatcg ccagtctggc
    11341 tcccacggaa aatcaaacac tttcgcctgc aacactgcca gtgccgtttc cgcaccaatt
    11401 gcggccacca ggccggaaac aaacccaagc atggcgaact cacaccacaa tgtcgtgcgc
    11461 agcagttttt tccccgcacc gagtgtgcgc cataccacca gctcctgatg acgctggcgc
    11521 attcccacct gcacctgtgc cagcaacagc aacataccgc aggcggtgac cagcaccacc
    11581 atcacctcca gcgcccgact tacctgctcc agcacctgac cgacctgttt taaaatcgcg
    11641 ccaatatcta acaggctaat ggtcgggaac tggcggttga gttgcgtcaa catgccgttg
    11701 ccattctccc agcggaaact ggtaagccag ctctgcggtt gcccgtctaa tgccccttcc
    11761 gggaaaataa aatagaaatt aggccgcaga ctttcccagt ccactttgcg caggctggtc
    11821 actttagcgc ggaactcctg ggtatcgccc ataaaagtca cggtatcacc gagggcaacg
    11881 tttaagcgtt tcgccagccc ctcttccatc gacacttcat cggcttttgg cggccagtta
    11941 ccggcgacaa tcggattatg atcgggccgc gtattttgcc aggtaagatt gagttcgcga
    12001 ttaagcgcct catcttcatt accttctgtc ggcttatcgt taatcgccgt cagccgcgcc
    12061 cgcactaccg gataaaatga ttccgggact atatga
//
LOCUS       DAMZET010000127        12033 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.127, whole genome shotgun sequence.
ACCESSION   DAMZET010000127 DAMZET010000000
VERSION     DAMZET010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.127"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..537
                     /locus_tag="POB34_003500"
     CDS             <1..537
                     /locus_tag="POB34_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDI5920385.1"
                     /translation="GVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHR
                     MAAQQISIASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYA
                     QLYIRPNEQNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARIS
                     PRTATFHKGQLVWGSVAG"
     gene            914..1963
                     /gene="yahK"
                     /locus_tag="POB34_003501"
     CDS             914..1963
                     /gene="yahK"
                     /locus_tag="POB34_003501"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HDI5920386.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            2206..3021
                     /locus_tag="POB34_003502"
     CDS             2206..3021
                     /locus_tag="POB34_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920387.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            3433..3678
                     /locus_tag="POB34_003503"
     CDS             3433..3678
                     /locus_tag="POB34_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920388.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(3695..4366)
                     /locus_tag="POB34_003504"
     CDS             complement(3695..4366)
                     /locus_tag="POB34_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HDI5920389.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGNAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            4513..4788
                     /locus_tag="POB34_003505"
     CDS             4513..4788
                     /locus_tag="POB34_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDI5920390.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(4886..6472)
                     /gene="prpR"
                     /locus_tag="POB34_003506"
     CDS             complement(4886..6472)
                     /gene="prpR"
                     /locus_tag="POB34_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HDI5920391.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCKLEEDMQQGRFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            6710..7600
                     /gene="prpB"
                     /locus_tag="POB34_003507"
     CDS             6710..7600
                     /gene="prpB"
                     /locus_tag="POB34_003507"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HDI5920392.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEAKLDDLFARSQVK"
     gene            7645..8814
                     /gene="prpC"
                     /locus_tag="POB34_003508"
     CDS             7645..8814
                     /gene="prpC"
                     /locus_tag="POB34_003508"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HDI5920393.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            8848..10299
                     /gene="prpD"
                     /locus_tag="POB34_003509"
     CDS             8848..10299
                     /gene="prpD"
                     /locus_tag="POB34_003509"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HDI5920394.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            10339..>12033
                     /gene="prpE"
                     /locus_tag="POB34_003510"
     CDS             10339..>12033
                     /gene="prpE"
                     /locus_tag="POB34_003510"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HDI5920395.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMAL"
BASE COUNT         2997 a         3024 c         3207 g         2805 t
ORIGIN      
        1 ggcgtggcag ccatcaatta tatggttgaa acggtagaga aaacgccaca gctgaagggc
       61 aagctgacca tcagtcacgc ctttgcgctg gcaacgctca acgaacaaca ggtagatgaa
      121 ctggcgcacc ggatggcggc gcagcaaatt tctatcgcct cgacggtgcc gattggcacg
      181 ctgcatatgc cgctcaaaca gttgcacgac aaaggcgtaa aagtgatgac tggcactgac
      241 agcgttatcg accactggtc gccttacggc ctgggcgaca tgctggaaaa agccaatctg
      301 tacgcgcagc tctatattcg tcctaacgaa cagaacctct cccgctcgct gtttttagcc
      361 actggcgatg tattgccgct caacgaaaaa ggcgagcgcg tgtggcccaa agcgcaggat
      421 gacgccagtt ttgtgctggt ggacgcctcc tgttccgccg aggcggtggc gcgtatctcg
      481 ccgagaaccg caacgttcca taaagggcaa ctggtgtggg ggagtgtggc aggttgatgc
      541 gcatttgaaa cgcctgcaac ggtgaatagt aagagattta agccccaggg agtggggcaa
      601 cctgaatatg agtgtgtcgg cagaaatgga tattatcttt gaggaatatg cccttattga
      661 cgttgtgtag ataactattt gtctacgtga aaagatcatc agttttgccg attttagtct
      721 tttacggata gcaaatatca cacttacagg tgctcgcctt atggggaacg ttgctgcttt
      781 gtttcattca ggctcgcggc atgttggggc tggatcgggt ggtttaaata ttggctatat
      841 tcaatggacg cgttttgccg cgatgacata tcaggcatcg ccaaatacac atagctaatc
      901 aggagtaaac acaatgaaga tcaaagctgt tggtgcatat tccgctaaac aaccacttga
      961 accgatggat atcacccggc gtgaaccggg accgaatgat gtcaaaatcg aaatcgctta
     1021 ctgtggcgtc tgccattccg atctccacca ggtccgttcc gagtgggcgg ggacggttta
     1081 cccctgcgta ccgggtcatg aaattgtggg gcgtgtggta gccgttggtg atcaggtaga
     1141 aaaacatgcg ccgggcgatc tggtcggtgt cggctgcatt gtcgacagtt gtaaacattg
     1201 cgaagagtgt gaagacgggc tggaaaacta ctgtgatcac atgaccggca cctataactc
     1261 gccgacgccg gacgaaccgg gccatactct gggcggctac tcacaacaga tcgtcgttca
     1321 tgagcgatat gttctgcgta ttcgtcaccc gcaagagcag ctggcggcgg tggctccttt
     1381 gttgtgtgca gggatcacca cgtattcgcc gctacgtcac tggcaggccg ggccgggtaa
     1441 aaaagtgggc gtggtcggca tcggcggtct gggacatatg gggatcaagc tggcccacgc
     1501 gatgggggcg catgtggtgg catttaccac ttctgaggca aaacgcgaag cggcaaaagc
     1561 cctgggggcc gatgaagttg ttaactcacg caatgccgat gagatggcgg ctcatctgaa
     1621 gagtttcgat ttcattttga atacagtagc tgcgccacat aatctcgacg attttaccac
     1681 cttgctgaag cgtgatggca ccatgacgct ggttggtgcg cctgcgacac cgcataaatc
     1741 gccggaagtt ttcaacctga tcatgaaacg ccgtgcgata gccggttcta tgattggcgg
     1801 cattccagaa actcaggaga tgctcgattt ttgcgccgaa catggcatcg tggctgatat
     1861 agagatgatt cgggccgatc aaattaatga agcctatgag cgaatgctgc gtggtgatgt
     1921 gaaatatcgt tttgttatcg ataatcgcac actaacagac tgaaaaaatt aataaatacg
     1981 ctgtggttta acatattaac ttcgctctcc acttaacttt ttagttaagg agagcgaaat
     2041 aatatcaaag tagcagtaaa acctataacg taaattcaaa ttgttaaatt aactccctcc
     2101 agtacacaat acttcacacg ttagttatga gcgatttctg atagtgcctg gtttaatcag
     2161 agctttatta tctgcgacgt ttatttttat ttaagagaga tcgcgatgat atcattaaaa
     2221 gctccgcaca ataatttgat gccatatacg caacaaagca tacttaatac ggttaaaaac
     2281 aatcagttac cagaggatat caaaagctcc ctggtttcct gtgtcgatat attcaaggtt
     2341 ttgattaaac aatattatga ttatccttat gattgtcgtg atgatttagt cgatgacgac
     2401 aaactcattc atctcatggc tgccgtacga gactgtgagt ggtcagacga taacgcactc
     2461 accataaatg tgcagtttaa tgatttccct ggattttatg actggatgga ttaccctgat
     2521 cacccggtta agtttgtttt tcgcatactt gaaaatcaaa aagggacagt ttgggtatat
     2581 gaccaggatg atgcgtttct tgatattaaa gcaaatgtgc aggctggacg ctttaccggg
     2641 cttaagaaat tagttcagtt tatcgattca gtgcgaacag attgcaaatg tattcttctt
     2701 gaacaccata tgcctttgct gagacttttc cctaaaggga aagagtgtat gcatgtggaa
     2761 aaatggcttc gcgaaatgtc gtctattccg gagacagacg cgcccattaa gcaggcgctt
     2821 gcgcatggat tattattaca cttaaaaaat atctaccctg tttttcctga atcactggtg
     2881 atgttattgc tctctgttct ggacgtaaaa acatacagag atgacgcacg attaaatgaa
     2941 tggatatcga atagagtgca ggaattaggt gacagatatt atcctgtaaa taagcacgtt
     3001 aaaatccgct acactcttta gttcagaaag ggttccacct gcaagttgtt attccaccag
     3061 agagtgaatc gcaaagcagg taatcatttc atctgtatat ccgtatgcta aatggcattc
     3121 caccgccttt tcgccgatat tttgttgtga tcagcgacgc ttcgcttaaa aaagcaccag
     3181 cagtggtttc gcagctatgt ggtgtataaa aaatgatctc atgcagatgt tttgtgaatg
     3241 tgtgggttga cattcatatg aaaaaaatca taattccatc atgtttgtat ggggtctttt
     3301 ctgtacctta cgcatcgcac tcaagcctga cagaaaatat gctgtaaggc tcatatcaaa
     3361 aaccgccatt agctcatcag gaagagcaga cgacaaccag gattgttgta tggtacgggg
     3421 ttcgaggcct cgatggcggt ccaacttttc aaaactcttc tgaatcagat ccccttatta
     3481 tcatccctgc aaagtggaac attgccattg tttggctatt caggacgggt cagaccaatg
     3541 aaaaaagccc acacagggga gagtgggctg aaatgggaag ctaaagactc aagtaaactt
     3601 atcggaaata aggaccacgc attacggggg ctatcatcgc cgatggtcgg gattcggcaa
     3661 atcaggttga ttacgtagag agtaaattat ctgctcaccg ctgcgtcacc ccttcgtaaa
     3721 tcaggcgtag cgcgaataca ccaataattg caccaataac ccgactggca acgcgttgca
     3781 tacgcccata agcacgacgc acagcgggca aagaaaacgc ctgactaaga aaaactcgcc
     3841 agataattga tgcgagcaca atccccgccc aggccattaa acgtgcccat gttggtgttt
     3901 cggcatttaa tgttactgag aaaatactga taaaaaataa aacggtttgc gggttagaga
     3961 gatcggtaat taatccgcgg cgaaaaaaga cataccaggg ggcgctaatc ggttgttgta
     4021 gtgtgctcat ttgcggtgtt gactggcggc gcatgctgca ccacgcaaac cataagagat
     4081 aagcgccgcc gacgattctg ataagcgaaa aaatctcctc acactgcgta attagcgttg
     4141 caagaccaaa caaacccaac ccggaataaa atgcattgcc cagcgccacg cccagcccgg
     4201 tcagcacccc tgcgcgtcga ccggaagcca ggctggtttg tactaccaca aagagattgg
     4261 ctcccggatt aaaaaaagta atcacgaaca gtcctacggt caggtaaacg gcgtgcaaag
     4321 gatccatagt gatttcatcc ataaataagt gaagtaactt catcatattt ctaccaaaaa
     4381 taatcgggtg cgagagagat cacaaagtgt cttatttccg gttactggcg tttatgccct
     4441 gactgaacta attattaatc aacccaataa tgtgggtgga tgatagtgtg ataacaactc
     4501 tggagccgta atatgaaaat aatctctaaa atgttagtcg gtgcgttagc gttagccgtt
     4561 accaatgtct atgccgctga attgatgacc aaagcggaat ttgaaaaagt tgaatcgcag
     4621 tatgaaaaaa taggtgatat ttcaaccagc aatgaaatgt cgactgcaga tgcaaaagaa
     4681 gatttgatca aaaaagcgga tgaaaaaggg gctgatgtgt tggtactgac ctccggtcaa
     4741 actgacaata agatccacgg cacggcaaat atttataaga agaagtaatt ctgaacccta
     4801 tgtaaacatg cccgatgcgt aagtttatcg gtgatcatct attgaaattt atgccggata
     4861 aagcgttcgc gctgcattcg gcagttcagc ttttcagccg ccgccagaac gtcgtccggc
     4921 tgatgcctaa ataattcgcc gctgctgttt tatcgccatt aaatttctcc agtgcctgtt
     4981 gtggtgtcag taagcgtgga gcgggagttt tcgccgactc gcgcgccagt tccggcagta
     5041 gcagctgcaa aaattgcggc gttaaatccg gcgtcggttc cacacttaaa aatagcgcca
     5101 gtcgctccat catattgcgc agttcacgaa tattacctgg ccagtcgtag tgcaccagca
     5161 cggtttcgct tgcctgtaat ccctggcgta atgcggcaga aaacggggcg gagagcgccg
     5221 ccagagacac tttcaaaaag ctttccgcca gcggaagaat atccgccacc cgctcgcgca
     5281 gtggtggcaa ttgcagacgc aaaatactca gccgataaaa cagatcacgg cgaaaacgtc
     5341 cttgctgcat atcttcttcc agcttgcagt gagtggcgct aatgacccgt acatctaccg
     5401 gaacaggctg atgcccgccg acgcgggtga cctctttttc ttccagcacc cgtaacaggc
     5461 gagtctgcaa aggtagcggc atttcgccaa tctcatccag aaacagcgta ccgccgtggg
     5521 caatttcgaa cagcccggcg cgacctccgc gtcgcgagcc ggtaaacgcc ccttcctcat
     5581 agccaaacag ctccgcttcc agcagcgatt cggctatcgc cccgcagttg acggcaacaa
     5641 acggatgcga ctttttgccc tgtcgcgcat cgtggcgggc aaaatattcc cgatgaatcg
     5701 cctgggccgc cagctctttg cccgtccccg tttccccctc aatcaacacc gctgcactgg
     5761 agcgggcata cagcaaaata gtctgccgta cttgttccat ctgtggtgat tgaccgagca
     5821 tatcgcccag cacgtaacga gttctcaggg cgttgcgggt ggcatcgtga gtgttatggc
     5881 gtaacgacat gcgcgtcata tccagcgcat cgctgaacgc ctggcgcacg gtggcggcgg
     5941 aatagataaa aattccggtc attccggctt cttctgccag atcggtaatc agccccgcgc
     6001 cgaccaccgc ttcagtgccg ttagctttca gctcgttaat ctgcccgcgg gcgtcttctt
     6061 cggtaatgta gctacgttga tcgaggcgca gattaaaggt tttttgaaac gccaccagcg
     6121 ctggaatggt ttcctgatag gtgacaacgc cgatagaaga ggtgagtttt ccggcttttg
     6181 ccagtgcctg taacacatcg tagccgctcg gtttaatcaa aataactggc actgacaggc
     6241 ggcttttcag gtacgcgccg ttagagccag cagcgatgat ggcgtcacag cgttcgtttg
     6301 ccagtttctt gcggatgtag gtcactgctt tttcaaagcc aagctgaata ggggtaatgt
     6361 tcgccaggtg atcaaactcg aggctgatat cgcgaaacag ctcgaacagg cgcgttacag
     6421 ataccgtcca gataaccggt ttgtcgtcat taagccgtgg tggatgtgcc atagcgcacc
     6481 gcaaagttaa gaaaccgaat attgggttta gtcttgtttc ataattgttg caatgaaacg
     6541 cggtgaaaca ttgcctgaaa cgttaactga aacgcatatt tgcggattag ttcatgacgt
     6601 tatctctaac aaattgaaat taaacattta attttattaa ggcaattgtg gcacacccct
     6661 tgctttgttt tattaacgaa aataacaagt tgataacaaa ggatgggcta tgtctctaca
     6721 ctctccaggt aaagcgtttc gcgctgcact gactaaagaa aatccattgc agattgttgg
     6781 caccatcaac gctaatcatg cgctgttggc gcagcgtgcc ggatatcagg caatttatct
     6841 ctccggcggt ggcgtggcgg cagggtcgct ggggctgccc gatctcggta tttctaccct
     6901 tgatgatgtg ctgacagata ttcgccgtat caccgacgtt tgttcgctgc cactgctggt
     6961 ggatgcggat atcggttttg gttcttcggc ctttaacgtg gcgcgtacgg tgaaatcgat
     7021 gattaaagcc ggtgcggcag gattgcatat tgaagatcag gttggtgcga aacgctgcgg
     7081 tcatcgcccg aacaaagcga tcgtctcgaa agaggagatg gtggatcgga tccgcgcggc
     7141 ggtggatgcg aaaaccgatc ctgattttgt gatcatggcg cgcaccgatg cgctggcggt
     7201 agaggggctg gatgcggcga tcgagcgtgc acaggcctat gttgaagcgg gtgccgagat
     7261 gttgttcccg gaggcgatta ccgaactcgc catgtaccgc cagtttgccg atgcggtgca
     7321 ggtgccgatc ctcgccaaca ttaccgaatt tggcgcaaca ccgctgttta ccaccgacga
     7381 attacgcagc gcccatgtcg caatggcgct gtacccactt tcagcgttcc gcgccatgaa
     7441 ccgcgccgct gagcatgtct acaacgtcct gcgccaggaa ggcacgcaga aaagcgtcat
     7501 cgataccatg caaacccgca acgagctgta cgaaagcatc aactactacc agtacgaagc
     7561 gaagctcgac gacctgtttg cccgtagcca ggtgaaataa aacacaacga aaaatatctc
     7621 gaccctacaa atgacgagga caacatgagc gacacaacga tcctgcaaaa cagtacccat
     7681 gtcattaaac cgaaaaaatc tgtggcactt tctggcgttc cggcgggcaa tacggcgctc
     7741 tgcaccgtgg gtaaaagcgg caatgacctg cattaccgcg gctacgatat tcttgatctg
     7801 gcggaacatt gcgaatttga agaagtggcg catctgctga tccacggcaa actgccgacc
     7861 cgtgacgaac tcgccgctta caaaacgaaa ctgaaagccc tgcgcggttt accggctaac
     7921 gtgcgtaccg tgctggaagc cttaccggcg gcgtcacacc cgatggatgt tatgcgcacc
     7981 ggcgtttccg cgctcggctg cacgctgcca gaaaaagagg gacataccgt ctctggcgcg
     8041 cgggatattg ccgacaaact actggcatca cttagctcga ttctccttta ttggtatcac
     8101 tacagtcaca acggcgaacg catccagccg gaaaccgatg acgactccat cggtggtcac
     8161 ttcctgcatc tgctgcacgg cgaaaagcca tcgcaaagct gggaaaaggc gatgcatatt
     8221 tcgctagtgc tgtacgccga acacgagttt aacgcctcca cctttaccag tcgggtgatt
     8281 gcgggcactg gctctgatat gtattccgcg attattggcg cgattggcgc actgcgcggg
     8341 ccgaaacacg gcggagcgaa tgaagtgtcg ctggagatcc agcaacgcta cgaaacgccg
     8401 gacgaagccg aagccgatat ccgcaagcgg gtggaaaaca aagaagtggt cattggtttt
     8461 ggtcatccgg tttataccat cgccgacccg cgccaccagg tgatcaaacg tgtggcgaag
     8521 cagctctcgc aggaaggcgg ctcgttgaag atgtacaaca tcgccgatcg cctggaaacg
     8581 gtgatgtggg agagcaaaaa gatgttcccc aatctcgact ggttctctgc tgtttcctac
     8641 aacatgatgg gcgttcccac cgagatgttc acaccactgt ttgtcatcgc ccgtgtcacc
     8701 ggctgggcgg cgcacatcat tgaacaacgt caggacaaca aaattatccg tccttccgcc
     8761 aattatgttg gaccggaaga ccgccagttt gtcgcgctgg ataagcgcca gtaaacctct
     8821 acgaataaca ataaggaaac gtacccaatg tcagctcaaa tcaacaacat ccgcccggaa
     8881 tttgatcgtg aaatcgttga tatcgtcgat tacgtgatga actacgaaat cagctctaaa
     8941 gtggcctacg acaccgcaca ttactgcctg ctcgacacgc tcggctgcgg tcttgaagct
     9001 ctcgaatacc cggcctgtaa aaaactgctg gggccaattg ttcctggcac cgtcgtaccc
     9061 aacggcgtgc gcgtccccgg aactcagttt cagctcgacc ccgtccaggc ggcatttaac
     9121 attggcgcga tgatccgctg gctcgatttc aacgatacct ggctggcggc ggagtggggg
     9181 catccttccg acaacctcgg cggcattctg gcaacggcag actggctttc gcgcaacgcg
     9241 gtcgccagcg gcaaagcgcc gttgaccatg aaacaggtgc tgaccgcaat gatcaaagcc
     9301 catgaaattc agggctgcat cgcgctggaa aactccttta accgcgtcgg cctcgaccac
     9361 gttctgttag tgaaagtggc ttccaccgcc gtggtcgccg aaatgctcgg cctgacccgc
     9421 gaggaaattc tcaacgccgt ttcgctggcg tgggtggacg gtcagtcgct gcgcacctat
     9481 cgccatgcgc cgaacaccgg cacgcgtaaa tcctgggcgg cgggcgatgc cacttcccgc
     9541 gcggtacgtc tggcactgat ggcgaaaacg ggcgaaatgg gttacccgtc agccctgacc
     9601 gcgccggtat ggggcttcta cgacgtctcc tttaaaggtg aatcgttccg cttccagcgt
     9661 ccgtacggtt cttacgtcat ggagaatgtg ctgttcaaaa tctccttccc ggcggagttc
     9721 cactcccaga cggcagttga agcggcgatg acgctctatg aacagatgca ggcagcaggc
     9781 aagacggcgg cggatatcga aaaagtgacc attcgcaccc acgaagcctg tattcgcatc
     9841 atcgacaaaa aggggccgct caataacccg gctgaccgcg accactgcat tcagtacatg
     9901 gtggcgatcc cgctgttatt cgggcgctta acggcggcag attacgagga caacgttgcg
     9961 caagataaac gcatcgacgc cctgcgcgag aagatcaatt gctttgaaga tccggcattt
    10021 accgctgact accacgaccc ggaaaaacgc gccatcgcca atgccattac ccttgagttc
    10081 accgacggca cacgatttga agaagtggtg gtggagtacc ccattggtca tgctcgccgc
    10141 cgtcaggatg gtattccgaa actggtcgat aaattcaaaa tcaatctcgc gcgccagttc
    10201 ccgactcgcc aacagcagcg cattctggag gtttctctcg acagaactcg cctggaacag
    10261 atgccggtca atgagtatct cgacctgtac gtcatttaag taaacggcgg taaggcgtaa
    10321 gttcaacagg agagcattat gtcttttagc gaattttatc agcgttcgat taacgaaccg
    10381 gagcagttct gggccgagca ggcccggcgt attgactggc agacgccctt tacgcaaacg
    10441 ctcgaccaca gcaatccgcc gtttgcccgt tggttttgtg aaggccgaac caatttgtgc
    10501 cacaacgcta tcgaccgctg gctggagaaa cagccagagg cactggcatt gattgccgtc
    10561 tcttcggaaa cagaagaaga gcgcaccttt acctttcgcc agctgcatga cgaagtgaac
    10621 gcggtggcct caatgttgcg ctcactgggc gtgcagcgcg gcgatcgggt gctggtgtat
    10681 atgccgatga ttgccgaagc gcatattact ctgctggcct gcgcgcgcat tggtgctatt
    10741 cattcggtgg tatttggtgg ttttgcctcg cacagcgtgg cggcgcgaat tgatgacgct
    10801 aaaccggtgc tgattgtctc ggctgatgcc ggagcgcgcg gtggcaaaat cattccctat
    10861 aaaaaattgc tcgacgatgc gataagtcag gcgcagcacc agccacgcca tgttttgctg
    10921 gtggatcgcg ggctggcgaa aatggcgcgc gtcagcgggc gggatgtcga tttcgcgtcg
    10981 ttgcgccatc aacacatcgg cgcgcgggta ccggtggcgt ggctggaatc caacgaaacc
    11041 tcctgcattc tctacacttc cggcacgacc ggcaaaccta aaggcgtgca gcgtgacgtc
    11101 ggcggatatg cggtggcgct ggcgacctcg atggacacca tttttggcgg caaagcgggc
    11161 agcgtgttct tttgcgcatc ggatatcggc tgggtggtgg ggcattcgta tatcgtttac
    11221 gcgccgctgc tggcggggat ggcgactatc gtttacgaag gattgccgac ctggccggac
    11281 tgcggcgtgt ggtggaaaat cgtcgagaaa tatcaggtta gccggatgtt ctcagcgccg
    11341 accgccattc gcgtgctgaa aaaattccct accgctgaaa ttcgcaaaca cgatctctcg
    11401 tcgctggaag tgctctatct ggctggagaa ccgctggacg agccgaccgc cagttgggtg
    11461 agcaatacgc tggatgtgcc ggtcatcgac aactactggc agaccgaatc cggctggccg
    11521 attatggcga ttgctcgcgg tctggacgac aggccgacgc gtctgggaag ccccggcgtg
    11581 ccgatgtatg gctataacgt gcagttgctt aatgaagtca ccggcgaacc gtgtggcgtc
    11641 aacgagaaag ggatgctggt ggtggaaggg ccgctgccgc cgggctgtat tcagaccatc
    11701 tggggcgacg acgaccgctt tgtgaagact tactggtcgc tgttttcccg cccggtgtac
    11761 gccacctttg actggggcat ccgtgacgct gacggttatc actttattct cgggcgcact
    11821 gacgatgtaa ttaacgttgc cgggcatcgt ctgggcacgc gcgagattga agagagtatc
    11881 tccagccatc cgggcgttgc cgaagtggcg gtggttgggg tgaaagatgc gctgaaaggg
    11941 caggtggcgg tggcgtttgt cattccgaaa gagagcgata gtctggaaga tcgtgatgtg
    12001 gcgcattcgc aagagaaggc gattatggcg ctg
//
LOCUS       DAMZET010000128        11980 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.128, whole genome shotgun sequence.
ACCESSION   DAMZET010000128 DAMZET010000000
VERSION     DAMZET010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.128"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            15..212
                     /locus_tag="POB34_003511"
     CDS             15..212
                     /locus_tag="POB34_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiQ"
                     /protein_id="HDI5920396.1"
                     /translation="MLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQ
                     GKTNELLSGKASASALLGITG"
     gene            complement(326..1090)
                     /locus_tag="POB34_003512"
     CDS             complement(326..1090)
                     /locus_tag="POB34_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDI5920397.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(1176..2054)
                     /gene="araC"
                     /locus_tag="POB34_003513"
     CDS             complement(1176..2054)
                     /gene="araC"
                     /locus_tag="POB34_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HDI5920398.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            2393..4093
                     /gene="araB"
                     /locus_tag="POB34_003514"
     CDS             2393..4093
                     /gene="araB"
                     /locus_tag="POB34_003514"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HDI5920399.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPCSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            4104..5606
                     /gene="araA"
                     /locus_tag="POB34_003515"
     CDS             4104..5606
                     /gene="araA"
                     /locus_tag="POB34_003515"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDI5920400.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            5806..6501
                     /gene="araD"
                     /locus_tag="POB34_003516"
     CDS             5806..6501
                     /gene="araD"
                     /locus_tag="POB34_003516"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDI5920401.1"
                     /translation="MLEDLKRLVLEANLALPKHNLVTLTWGNVSAVDRERGVLVIKPS
                     GVDYSVMTADDMVVVSIATGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            6576..8927
                     /gene="polB"
                     /locus_tag="POB34_003517"
     CDS             6576..8927
                     /gene="polB"
                     /locus_tag="POB34_003517"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HDI5920402.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDIRNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYIRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            9092..>11980
                     /gene="rapA"
                     /locus_tag="POB34_003518"
     CDS             9092..>11980
                     /gene="rapA"
                     /locus_tag="POB34_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HDI5920403.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLI"
BASE COUNT         2817 a         3308 c         3259 g         2596 t
ORIGIN      
        1 cgttacgtca ggagatgctg acgctggtga gcacgagctg ccagcagcaa aaaatgacgc
       61 tattgatggt gtcgcacagt gtggaagatg cggcgcggat cgccacacgc tcggtggtgg
      121 tcgccgacgg gcgcatcgcc tggcagggta agaccaatga gttgttgagc ggtaaggcga
      181 gtgcttcggc actattgggg attacgggtt agtatgcact gtcggatgcg gcgtgaacgc
      241 cttatccgac ctacgggtag tgcgttttgt aggcctgata agacgcggtc agcgtcgcat
      301 caggcaaccc gcacaagact atctcctaac ccccaaccac tttacgcaaa atatcgatat
      361 acaccggcat caacgggtgg cgaattaaca ccaccagcgc caccacgccg atggcagaaa
      421 ttaacggcgt cagccacaac aaacggccgc gcgacaaata atgactcaaa cggtcagtcg
      481 ctttaccact gcgccataac cgccagcaca gccagccacc aacccacaaa aacaccgctg
      541 ttgccagcag caaccattta aactcaccgc tctgcattcc ggctggaata tcgatcgccg
      601 cgcccgccag aatccctggc aggaagtaaa acggtggcca cagcaggcag ccgataatat
      661 tcggcgtaat gaattttgcc accggcagat ccagcattcc cgccaccatt ggcaccagcg
      721 gacgcgtcgg gccaacaaaa cgaccgacca gaatggtgaa catgctgtgt tgatgcaacg
      781 catgttcagt tttatcgagt aatgctttgt ttttcttcag aaatgaccag cgatgcaacg
      841 gctttttaaa acgccagccc agccagaagg agatccagtc gcccagcagg caaccgacga
      901 tccctgccag ccaggcgtgc cagaaactta actcgccgct gccaatcagc gctcccagcc
      961 ccgccatcag caccgtaccg ggtagaatca aaccgaccag cgccagcgac tccagaaagg
     1021 ccaccaacac caccgccatc aatgaataca cggtagattg ggtaataaag tgttccagca
     1081 atgcttgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
     1141 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
     1201 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
     1261 aatactcgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
     1321 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
     1381 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggtgacaag
     1441 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
     1501 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
     1561 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
     1621 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
     1681 gcttcatccg ggcgaaagaa acccgtattg gcaaatattg acggccagtt aagccattca
     1741 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
     1801 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
     1861 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
     1921 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
     1981 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
     2041 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
     2101 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac ccaaccggta
     2161 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
     2221 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
     2281 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
     2341 tcgcaactct ctactgtttc tccatacccg tttttttgga tggagtgaaa cgatggcgat
     2401 tgcaattggc ctcgattttg gcagtgattc tgtgcgagct ttggcggtgg actgcgccag
     2461 cggtgaagag atcgccacca gcgtagagtg gtatccccgt tggcaaaaag ggcaattttg
     2521 tgatgccccg aataaccagt tccgtcatca tccgcgtgac tacattgagt caatggaagc
     2581 ggcactgaaa accgtgcttg cagagcttag cgtcgaacag cgcgcagctg tggtcgggat
     2641 tggcgttgac acaaccggct cgacgcccgc accgattgat gccgacggta acgtgctggc
     2701 gctgcgcccg gagtttgccg aaaacccgaa cgcgatgttc gtattgtgga aagaccacac
     2761 tgcggttgaa gaagcggaag agattacccg tttgtgccac gcgccgggca acgttgacta
     2821 ctcccgctat attggcggta tttattccag cgaatggttc tgggcaaaaa tcctgcatgt
     2881 gactcgccag gacagcgccg tggcgcaatc tgccgcatcg tggattgagc tgtgcgactg
     2941 ggtgccagct ctgctttccg gtaccacccg cccgcaggat attcgtcgcg gacgttgcag
     3001 cgccgggcat aaatctctgt ggcacgaaag ctggggcggc ctgccgccag ccagtttctt
     3061 tgatgagctg gacccgatcc tcaatcgcca tttgccttcc ccgctgttca ctgacacctg
     3121 gactgccgat attccggtgg gcaccttatg cccggaatgg gcgcagcgtc tcggcctgcc
     3181 tgaaagcgtg gtgatttccg gcggcgcgtt tgactgccat atgggcgcag ttggcgcagg
     3241 cgcacagcct aacgcactgg taaaagttat cggtacttcc acctgcgaca ttctgattgc
     3301 cgacaaacag agcgttggcg agcgggcagt gaaaggtatt tgcggtcagg ttgatggcag
     3361 cgtggtgcct ggatttatcg gtctggaagc aggccaatcg gcgtttggtg atatctacgc
     3421 ctggtttggt cgcgtactcg gctggccgct ggaacagctt gccgcccagc atccggaact
     3481 gaaagcgcaa atcaacgcca gccagaaaca actgcttccg gcgctgaccg aagcatgggc
     3541 caaaaatccg tctctggatc acctgccggt ggtgctcgac tggtttaacg gccgccgcac
     3601 accgaacgct aaccaacgcc tgaaaggggt gattaccgat cttaacctcg ctaccgacgc
     3661 tccgctgctg ttcggcggtt tgattgctgc caccgccttt ggcgcacgcg caatcatgga
     3721 gtgctttacc gatcagggga tcgccgtcaa taacgtgatg gcgctgggcg gcatcgcgcg
     3781 gaaaaaccag gtcattatgc aggcctgctg cgacgtgctg aatcgcccgc tgcaaattgt
     3841 tgcctctgac cagtgctgtg cgctcggtgc ggcgattttt gctgccgtcg ccgcgaaagt
     3901 gcacgcagac atcccctcag cccagcaaaa aatggccagt gcggtagaga aaaccctgca
     3961 accgtgcagc gaacaggcac aacgctttga acagctttat cgccgctatc agcaatgggc
     4021 gatgagcgcc gaacaacact atcttccaac ttccgccccg gcacaggctg cccaggccgt
     4081 tccgactcta taaggacacg ataatgacga tttttgataa ttatgaagtg tggtttgtca
     4141 ttggcagcca gcatctgtat ggcccggaaa ccctgcgtca ggtcacccaa catgccgagc
     4201 acgtcgttaa tgcgctgaat acggaagcga aactgccctg caaactggtg ttgaaaccgc
     4261 tgggcaccac gccggatgaa atcaccgcta tttgccgcga cgcgaattac gacgatcgtt
     4321 gcgctggtct ggtggtgtgg ctgcacacct tctccccggc caaaatgtgg atcaacggcc
     4381 tgaccatgct caacaaaccg ttgctgcaat tccacaccca gtttaacgcg gcgctgccgt
     4441 gggacagtat cgatatggac tttatgaacc tgaaccagac tgctcatggc ggtcgcgagt
     4501 tcggcttcat tggcgcgcgt atgcgtcagc aacatgccgt cgttaccggt cactggcagg
     4561 ataaacaagc ccatgagcgt atcggctcct ggatgcgtca ggcggtctct aaacaggata
     4621 cccgtcatct gaaagtctgc cgatttggcg ataacatgcg tgaagtggcg gtcaccgatg
     4681 gtgataaagt tgccgcacag atcaagttcg gtttctccgt caatacctgg gcggttggcg
     4741 atctggtgca ggtggtgaac tccatcagcg acggcgatgt taacgcgctg gtcgatgagt
     4801 acgaaagctg ctacaccatg acgcctgcaa cacaaatcca cggcgaaaaa cgacagaacg
     4861 tgctggaagc ggcgcgtatt gagctgggga tgaagcgttt cctggaacaa ggtggcttcc
     4921 acgcgttcac caccaccttt gaagatttgc acggtctgaa acagcttcca ggtctggccg
     4981 tacagcgtct gatgcagcag ggttacggct ttgcgggcga aggcgactgg aaaactgccg
     5041 ccctgcttcg catcatgaag gtgatgtcaa ccggtctgca gggcggtacc tcctttatgg
     5101 aggactacac ctatcacttc gagaaaggca atgacctggt gctcggctcc catatgctgg
     5161 aagtctgccc gtcgattgca gtagaagaga aaccgatcct cgacgttcag catctcggta
     5221 ttggtggtaa ggacgatcct gcccgcctga tcttcaatac ccaaaccggt ccagcgattg
     5281 ttgccagcct gattgatctc ggcgatcgtt accgtctgct ggttaactgt atcgacacgg
     5341 tgaaaacccc gcactccctg ccgaaactgc cggtggcgaa tgcgctgtgg aaagcgcaac
     5401 cggatctgcc aactgcttcc gaagcgtgga tcctcgctgg tggcgcgcac cataccgtct
     5461 tcagccatgc gctgaacctc aacgatatgc gccagttcgc cgagatgcac gacattgaaa
     5521 tcacggtgat tgataacgac acccgcctgc cagcgtttaa agacgcactg cgctggaacg
     5581 aagtgtatta cggatttcgt cgctaagtag ccacatcagg tatgtaacgc ctgatgcgac
     5641 gctgacgcgt cttatctggc ctacgcgctg tgtttttgta ggccggataa gcaaagcgca
     5701 tccggcattc aacgcctgat gcgacgctgg cgcgtcttat caggcctacg cgctgcgatt
     5761 ttgtagaccg gataagcaaa gtgcatccgg cacgaaggag tcaacatgtt agaagatctc
     5821 aaacgcctgg tattagaagc caacctggcg ctgccaaaac acaacctggt cacgctcaca
     5881 tggggcaacg tcagcgccgt tgatcgcgag cgcggcgtcc ttgtgatcaa accttccggc
     5941 gtcgattaca gcgtcatgac cgctgacgat atggtcgtgg ttagcatcgc aaccggtgaa
     6001 gtggttgaag gtacgaaaaa gccctcctct gacacgccaa ctcaccggct gctctatcag
     6061 gcattcccgt ccattggcgg cattgtgcat acgcactcgc gccacgccac catctgggcg
     6121 caggcgggtc agtcgattcc agcaaccggc accacccacg ccgactattt ctacggcacc
     6181 attccctgca cccgcaaaat gaccgacgca gaaatcaacg gtgaatatga gtgggaaacc
     6241 ggtaacgtca tcgtagaaac cttcgaaaaa cagggtatcg atgcagcgca aatgcccggc
     6301 gtcctggtcc attctcacgg cccatttgca tggggcaaaa atgccgaaga tgcggtgcat
     6361 aacgccatcg tgctggaaga ggtcgcttat atggggatat tctgccgtca gttagcgccg
     6421 cagttaccgg atatgcagca aacgctgctg gataaacact atctgcgtaa gcatggcgcg
     6481 aaggcatatt acgggcagta atgactgtat aaaaccacag ccaatcaaac gaaaccaggc
     6541 tataatcaag cctggttttt tattggattt tcaacgtggc gcaggcaggt tttatcttaa
     6601 cccgacactg gcgggatacc ccgcaaggga cagaagtctc cttctggctg gcgacggaca
     6661 acgggccgtt gcaggttacg cttgcaccgc aagagtccgt ggcgtttatt cctgccgatc
     6721 aggttccccg cgctcagcat attttgcagg gtgaacaagg ctttcgcctg acgccgctgg
     6781 cgttaaagga ttttcaccgc cagccggtgt atggccttta ctgtcgcgcc catcgccagt
     6841 tgatgaatta cgaaaagcgc ctgcgtgaag gtggcgttac cgtttacgag gccgatgtgc
     6901 gcccgccaga acgctatctg atggagcgtt ttatcacctc gccagtgtgg gtcgagggtg
     6961 atatacgcaa tggcgctatc gttaatgccc gtctgaaacc gcatcccgac tatcgtccgc
     7021 cgctcaagtg ggtttctatt gatattgaaa ccacccgcca cggtgagctg tactgcatcg
     7081 gcctggaagg ttgcgggcag cgcatcgttt atatgctggg gccagagaat ggcgacgcct
     7141 ccgcgctcga ttttgaactg gaatacgtcg ccagtcgccc gcagctactg gaaaaactca
     7201 acgcctggtt tgccaactac gatcctgatg tgatcatcgg ttggaacgtg gtgcagtttg
     7261 atctgcgaat gctgcaaaaa catgccgagc gttatcgtat tccgctgcgg ctggggcgcg
     7321 ataacagtga gctggagtgg cgcgagcacg gctttaaaaa cggtgtgttt tttgctcagg
     7381 ctaaaggtcg gctaattatc gacggtatcg aggcgctgaa atccgcgttc tggaatttct
     7441 cttcattctc gctggaaact gtcgctcagg agctattagg cgaaggaaaa tctatcgata
     7501 acccgtggga tcgaatggac gaaattgacc gccgtttcgc cgaagataaa cctgcgctgg
     7561 cgacttataa cctgaaagat tgcgagctgg tgacgcagat tttccataaa actgaaatca
     7621 tgccgttttt gctcgaacga gcaacggtga acggcctgcc ggtagaccgc cacgggggat
     7681 cggtggcggc gtttggtcat ctctattttc cccgtatgca ccgcgctggt tatgtcgcgc
     7741 ctaatctcgg cgaagtgccg ccacacgcca gccctggcgg ctacgtgatg gattcacggc
     7801 cagggcttta tgattcagtg ctggtgctgg actataaaag cctgtacccg tcgatcatcc
     7861 gcacctttct gattgatccc gtcgggctgg tggaaggcat ggcgcagcct gatccagagc
     7921 acagtaccga aggttttctc gatgcctggt tctcacgaga aaaacattgc ctgccggaga
     7981 ttgtgactaa catctggcac gggcgcgatg aagccaaacg ccagggtaac aaaccgctgt
     8041 cgcaggcgct gaaaatcatc atgaatgcct tttatggcgt gctcggcacc accgcctgcc
     8101 gcttcttcga tccgcggctg gcatcgtcga tcaccatgcg tggtcatcag atcatgcggc
     8161 aaaccaaagc gttgattgaa gcgcagggct acgacgttat ctacggcgat accgactcaa
     8221 cgtttgtctg gctgaaaggc gcacattcgg aagaagaagc ggcgaaaatc ggtcgtgcac
     8281 tggtgcagca cgttaacgcc tggtgggcgg aaacgctgca aaaacaacgg ctgaccagcg
     8341 cattagaact ggagtatgaa acccatttct gccgttttct gatgccaacc attcgcggag
     8401 ccgataccgg cagtaaaaag cgttatgccg gactgattca ggagggcgac aagcagcgga
     8461 tggtgtttaa agggctggaa accgtgcgca ccgactggac gccgctggcc cagcagtttc
     8521 agcaggagct atacctgcgt atcttccgta acgagccata tcaggaatac atccgcgaaa
     8581 ccatcgacaa actgatggcg ggtgaactgg atgcgcggct ggtttaccgt aaacgccttc
     8641 gccgtccgct gagcgaatat cagcgtaatg ttccgcctca tgtacgcgcc gctcgccttg
     8701 ccgatgaaga aaaccaaaag cgcggtcgcc ccttgcaata tcagaatcgc ggcaccatta
     8761 agtacgtatg gaccaccaac ggcccggagc cgctggacta ccaacgttca ccactggatt
     8821 acgaacacta tctgacccgc cagctacaac ccgtggcgga gggaatactc ccttttattg
     8881 aggataattt tgctacactt atgaccgggc aacttgggct attttgagca aaaaaaagag
     8941 ttcgccagat accattttga tgcgtgacga atgctttgcc atccagtacc atagcgccct
     9001 ttccattcct ggacctgaat aacgccacta cctcataagc acggtagcgg gtggttattg
     9061 cctgcaatta aagatataga gccgaacaca tatgcctttt acacttggtc aacgctggat
     9121 cagcgataca gaaagcgaat tgggacttgg aaccgttgtc gcggtggatg cgcgaactgt
     9181 cactttactt ttcccatcta ctggtgaaaa ccgtctgtac gcacgcagtg attcccccgt
     9241 gacccgcgtg atgttcaacc ctggtgatac cattaccagc catgacggct ggcagatgca
     9301 agtcgaagaa gtaaaagaag aaaatggctt gctgacctat atcggtactc gcctggatac
     9361 tgaagagtcc ggcgtagccc tgcgtgaagt tttccttgat agcaaactgg tgttcagcaa
     9421 accgcaggac cgtctgtttg ccgggcagat tgaccgtatg gaccgctttg cgctgcgtta
     9481 tcgcgcgcgt aagtattcca gcgaacagtt ccgtatgccg tacagcggcc tgcgcggtca
     9541 gcgtaccagc ctgattccgc atcagctcaa catcgctcat gatgttggtc gccgccacgc
     9601 gccgcgcgtc ctgctggctg acgaagtggg tttagggaaa accattgaag ccgggatgat
     9661 cctgcatcag caactgctct ctggcgctgc tgaacgtgtg ctgattatcg tcccggaaac
     9721 cttacagcat cagtggctgg tagaaatgct gcgccgtttc aacctgcgct ttgcgctatt
     9781 tgatgatgag cgttatgccg aagctcagca cgatgcttac aacccgtttg acaccgaaca
     9841 gctggtgatt tgctcgctgg attttgcccg tcgtagcaaa cagcgcctgg aacatctctg
     9901 tgaagccgaa tgggacctgc tggtggtcga tgaagcgcat cacctggtgt ggagcgaaga
     9961 tgcgccaagc cgtgaatatc aggccattga acaactggca gagcacgtgc cgggcgttct
    10021 gctgctgacc gcgaccccgg aacagctggg gatggaaagc cacttcgccc gtctgcgtct
    10081 gctggacccg aaccgtttcc acgattttgc gcagttcgtt gaagagcaga aaaattatcg
    10141 tccggttgcg gacgccgttg ccatgctgct ggcaggtaac aaactgagca atgacgaact
    10201 gaacatgctc ggcgagatga tcggcgagca ggatatcgag ccgctgttgc aggcagcaaa
    10261 cagcgacagc gaagatgccc agagcgcccg tcaggagctg gtttcgatgc tgatggatcg
    10321 ccacggcacc agccgcgtgc tgttccgtaa cacgcgtaac ggtgtgaaag gattcccgaa
    10381 acgcgagctg cacaccatta aactgccgct accgacgcag tatcagacgg ctattaaagt
    10441 ctccggcatt atgggcgcac gtaaaagtgc ggaagatcgt gctcgcgata tgctctaccc
    10501 ggagcgtatt tatcaggaat ttgaaggtga taacgccacc tggtggaact tcgatccgcg
    10561 cgttgagtgg ctgatgggct acctgaccag ccatcgctct cagaaagtgc tggtgatctg
    10621 cgccaaagct gccactgcgc tgcaactgga gcaggtactg cgcgaacgtg aaggtattcg
    10681 cgctgcggtg ttccacgaag gtatgtcgat tatcgaacgt gaccgcgctg ccgcctggtt
    10741 tgccgaagaa gacaccggcg cacaggtact gctgtgctca gaaatcggtt ctgaaggacg
    10801 taacttccag ttcgccagcc acatggtgat gtttgacctg ccattcaacc cggatctact
    10861 ggagcagcgt attggtcgtc tggatcgtat cggccaggcg cacgatattc agatccatgt
    10921 gccttatctg gagaaaaccg ctcagtcggt gctggtgcgc tggtatcacg aaggtctgga
    10981 tgcatttgag cacacctgcc cgaccggacg cactatttac gatagcgtat acaacgatct
    11041 gattaactat ctggcttcac cggatcaaac cgaaggcttt gacgatctga tcaaaaactg
    11101 ccgcgagcaa catgaagcgc tgaaagcaca gctggaacag ggtcgtgacc gcctgctgga
    11161 aatccactcc aacggtggcg aaaaagccca ggcactggca gaaagcattg aagagcagga
    11221 tgacgatacc aacctgatcg ccttcgccat gaacctgttc gatattatcg gtatcaatca
    11281 ggacgatcgc ggcgacaaca tgatcgtgct gacgccgtcc gatcatatgc tggtgccgga
    11341 cttccctggc ctgtcggaag atggcatcac catcaccttt gatcgtgaag tggcgctggc
    11401 gcgtgaagat gcacagttta ttacctggga gcatccgctg atccgcaacg gtctggatct
    11461 gattctttct ggcgataccg gtagcagcac gatttcactg ttaaaaaaca aagcgttgcc
    11521 ggtaggtacg ctgttggtgg aactgattta tgtggttgaa gcccaggctc cgaagcagtt
    11581 gcagctcaac cgcttcctgc caccgacgcc ggtacgtatg ctgctggata aaaacggcaa
    11641 caacctggcg gcgcaggtag agtttgaaac ctttaaccgc cagcttaacg cggttaaccg
    11701 tcacaccggc agcaaactgg ttaacgccgt gcagcaggat gttcacgcta tccttcaact
    11761 gggtgaagcg cagatcgaga aatctgcccg tgcattgatt gatgcagcgc gtaacgaagc
    11821 cgacgaaaaa ctgtctgccg agctgtctcg tctggaagct ctgcgtgcag tgaacccgaa
    11881 cattcgtgac gacgaactga ccgccattga gagcaaccgt cagcaggtaa tggaaagcct
    11941 ggatcaggca ggttggcgtc tggatgccct gcgtttgatc
//
LOCUS       DAMZET010000129        11928 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.129, whole genome shotgun sequence.
ACCESSION   DAMZET010000129 DAMZET010000000
VERSION     DAMZET010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.129"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(72..956)
                     /gene="xapR"
                     /locus_tag="POB34_003519"
     CDS             complement(72..956)
                     /gene="xapR"
                     /locus_tag="POB34_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HDI5920404.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
     gene            complement(997..1155)
                     /locus_tag="POB34_003520"
     CDS             complement(997..1155)
                     /locus_tag="POB34_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HDI5920405.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            complement(1208..2464)
                     /gene="xapB"
                     /locus_tag="POB34_003521"
     CDS             complement(1208..2464)
                     /gene="xapB"
                     /locus_tag="POB34_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HDI5920406.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKAVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVAH"
     gene            complement(2524..3357)
                     /gene="xapA"
                     /locus_tag="POB34_003522"
     CDS             complement(2524..3357)
                     /gene="xapA"
                     /locus_tag="POB34_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HDI5920407.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            3606..4370
                     /locus_tag="POB34_003523"
     CDS             3606..4370
                     /locus_tag="POB34_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920408.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAEFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(4409..5335)
                     /locus_tag="POB34_003524"
     CDS             complement(4409..5335)
                     /locus_tag="POB34_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI5920409.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            5425..6423
                     /locus_tag="POB34_003525"
     CDS             5425..6423
                     /locus_tag="POB34_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HDI5920410.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSTDKA"
     gene            complement(6420..6638)
                     /locus_tag="POB34_003526"
     CDS             complement(6420..6638)
                     /locus_tag="POB34_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HDI5920411.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(6640..8655)
                     /gene="ligA"
                     /locus_tag="POB34_003527"
     CDS             complement(6640..8655)
                     /gene="ligA"
                     /locus_tag="POB34_003527"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDI5920412.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(8726..9712)
                     /gene="zipA"
                     /locus_tag="POB34_003528"
     CDS             complement(8726..9712)
                     /gene="zipA"
                     /locus_tag="POB34_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HDI5920413.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            9942..10703
                     /gene="cysZ"
                     /locus_tag="POB34_003529"
     CDS             9942..10703
                     /gene="cysZ"
                     /locus_tag="POB34_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HDI5920414.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            10888..11859
                     /gene="cysK"
                     /locus_tag="POB34_003530"
     CDS             10888..11859
                     /gene="cysK"
                     /locus_tag="POB34_003530"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDI5920415.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
BASE COUNT         2964 a         3156 c         2833 g         2975 t
ORIGIN      
        1 tgcgatttgc gaaattgcct gatgcgcttc acttagcaga cgactatttc cggcaattcc
       61 tgtctcctca cctactgtgt caatgcagcc aacagcttaa ccatcgcggg cgtcacctgc
      121 tgtgtttcat aaacaatata taaatctgca gggatgcgct gtttgagcgg acggaaaatg
      181 acacctggcc agttcatttg tgcgtagctg tccgctatca atgtgatacc aatacccata
      241 ctgaccatgg cgagtaccgt ttgcggttca ttaacttcgc gaataacaac cggtgaaaat
      301 cccacctgct ggcaaactcg ctgcaaaaaa tcccagtcag tgtacacggg cggcattgta
      361 acaaaatact cgtcacgtag cgcttccagc ggaacggtgg aaaatgatga gagatgatgc
      421 tcttcaggca tcgccaccag aaacgccgat tcatgcaacc gtaagctggt aaaacctgtc
      481 ggtggttctg tcgccattcg ccagatcccg gcatcgagtt cacgtcgttc cagcagggcc
      541 atttgcatcg cgggcatctt ttcgcgaaaa agaacttcaa cgttaggatt ttccctgagg
      601 aatcgccgca taaccgggcg catccgtccc cacatagccg ttcccactac gccgagttca
      661 atccgccctg cttctccccg acctatttgt tcaacccgag ccaatacatt attagcattc
      721 accagcaatc gacgcgattc ttccatcaag attttgcccg cgtgtgtcag tacgacgctg
      781 cgcgaatggc gaataaaaag ctgcgtgccg agttgatttt ccagctcttt aatatgaata
      841 ctgagcggag gctgagacat atttaaacgc gctgctgcgc ggccaaaatg caactcttcc
      901 gctacggcaa gaaaataacg gagcaactta agatctgttc tgtatacgcg ttccataatt
      961 aagtgctcca tataataaat acatcgtatt aaattatcat ggcataaagt atttcaccac
     1021 cagatatccg acatacataa acacaatgga taatatacct ggtaatacat ttggtacagg
     1081 aataggcaag cgcacgattg taaacaaagc accaatgcca aaaccaacgc cggaagaaaa
     1141 taaaatatct ttcactaatt aacctttatc ataaaagcag ctctgaagag cagagctgcg
     1201 aatcctttta atgagccacc gctcgatgct ttatcttttc agggtcatga ttatatttaa
     1261 acccaaagaa aaatatcact gcgagaaaaa gagcatatcc tgcaaacacc agccagatag
     1321 tttgccagtc ttttacgcca tccaccgaaa agtaatctac tgccatgcca ctcagaatcg
     1381 agccaaccca tgcgccgaca ccatttacca tggtcataaa gagcccctgc gcgctggcac
     1441 gaatgctgga atcaacttcc tgttcgacaa ataccgaacc agaaatattg aagaaatcga
     1501 atgcacagcc ataaacaatc atcgacagca gcagcaaaat aaatccggtt gttgacggat
     1561 cgccataggc gaagaagcca aagcgcagcg tccaggccac catactcatc agcatgacgg
     1621 ctttaatgcc aaatcgcttt aaaaagaatg ggatagtcag tataaagccc acttctgcca
     1681 tctgtgaaac tgacagtaaa atggagggat atttcaccac aaaactgtca gcaaactccg
     1741 ggttacgggc gaaatcatgt aggaacggat taccaaaaac gttggtaatt tgcagtaccg
     1801 cacccagcat catggcaaag aggaaaaaga tggccatgcg tggattttta aacagcacga
     1861 aggcatccag acccagcttg ctggcaagcg atgtggtcgc ttttttctcc gcaaccggaa
     1921 tcttcggcaa agtcagcgca taagccgaca gcagcaatga cgcaccggac gcgatataca
     1981 gctgcagact actcaattcc agatgcagca ggcttactgc ccacatcgcg acaatgaacc
     2041 ccaccgtacc aaaaacgcga atgggcggga aagcggtcac cgggtcaagc cctgcctggg
     2101 caagacagga ataagagacg ctgttcgata acgcaatagt cggcataaac gccatcgcat
     2161 tgactaacat cacccaaaac atcatatccg gatcagttac ggatgccgca taaaaaagta
     2221 cgcccgcaca caccaggtga cacagcatgt atgcacgttc tgcgcgcagc catttgtctg
     2281 cgatgatccc cattatacca ggcataataa tcgcggcgat ccctttggaa ctgtaaacca
     2341 tgccaacatt agcgccggtg aaatgaaggg tattaatcat gtaagagccg agggtaacca
     2401 gccagctccc ccagataaaa tattgcaaaa aggacattac ctttaagcgc atcgcgatgc
     2461 tcataatcgt ttccttgtcg catcatttgg cctcacccgc gaggccgttt cttattgttt
     2521 ttctcaggca attttgcgca gaaagccaca aataaggttg atgaagttct gcttagagag
     2581 ctccgcagcc gccagcgttt gcgcatgtga caatttcaca tcgcttaaac cttccgccat
     2641 attggtaatc gcagagaccg caacgacttt aaggtcgcaa tggcgagctg aaataacctc
     2701 aggcaccaca gacataccaa caacatcccc accaataatt tgcatcatgc gaatttccgc
     2761 cgcagtctcg aaattcggcc ccggatacga gacgaacacg ccctccgtca gagggaaacc
     2821 ctcttctttc gccacttttt gtaacagtgc gcggtattcc gcatcgtagg catttgccag
     2881 tgagaagaag cgctctccaa aacgatcatc gttaagaccc accatcggcg taccgggcat
     2941 ggtgttgata tgatctttca atgcgaccag gctgcctgcc cccacttccg gacgcagcga
     3001 gcctgccgca ttggtgcaga acagtagctc gcagcccagc agcttaaagg tacgaattgc
     3061 gtcagtcata atggtcattc cacgaccttc gtagaaatgt ccgcgacctt tcatacatac
     3121 caccggtacg ccctggagat ggcccagcac taactccccc gcatgaccat gtaccgtact
     3181 taccgggaag cccggcagct tttcgtagga aattgcgaca gcgttctcaa tctgatcggc
     3241 cagcgcgccg agcccggaac ctaaaataaa ggccactcgt ggcgtgaaat caggtttata
     3301 agtcttgata atatctatgc aaaacagcgg gttatgagaa aattgaacct gagacataca
     3361 tatccttttt ctgtagggtg gaatctaacg cctttttata gcgaggttgt cccggctctt
     3421 accaatacac ttttctcacg cattcaatag cgattcgata tcgatggatt tatatctttt
     3481 ctctattaat ccacggtaaa aactgtattg gcgatattaa ttcctttgca ttaacaatat
     3541 gtcgttttta gcaaaccact ttattaagca atctactaat aacgatcggc atagggaatt
     3601 atgctatgaa aaaacatctt ttaactctga cactttcctc tatattagcg atacctgttg
     3661 tatctcatgc agaatttaaa ggcggttttg cagatatcgg cgtgcattat ctggactgga
     3721 ccagccggac taccgaaaaa tcgtcgacca aatcacacaa agatgatttt ggttatcttg
     3781 aatttgaagg tggcgctaac tttagttggg gagaaatgta cgggttcttc gactgggaaa
     3841 acttctataa cggtcgccat aataaaccag gtagcgagca gcgttatacc tttaaaaata
     3901 ccaaccgtat ttacctgggt gacaccggat ttaatctcta cttgcatgcg tatggcacct
     3961 acggttctgc gaatcgcgtg aatttccacg acgatatgtt cctgtacggt atcggctaca
     4021 atttcaccgg cagcggttgg tggttcaaac cgttctttgc taagcgttat acagatcaaa
     4081 cctattacac tggcgacaac ggctatgtcg ccggctgggt tgcaggttac aactttatgc
     4141 tgggcagcga gaaattcact cttaccaact ggaacgagta cgagtttgac cgtgacgcta
     4201 cctatgcagc gggtaatggc ggtaaagaag ggctgaatgg cgcagttgca ctgtggtgga
     4261 atgcaacatc acacattact acggggattc agtatcgata tgcggatgac aaactgggcg
     4321 aagattttta ccaggatgcg ataatctact ccatcaaatt taacttctga ttgattactg
     4381 ccggggctcc cggcagtatg aattcactct atatctgata caacggatcc ccttcccgac
     4441 cagccattag cgcattgccc tggtctcgta ccacgtccca taacgcttca gcggcggtgg
     4501 aaagtgaacg gttcttcctg cgcaccagca tcaactggcg ttcaacaacc ggcgtaatgc
     4561 gctttaccac cagtgggcta ccttcaggca atggtagcgc cagtgcggga agaatactga
     4621 tgccgatgcc tgcggctacc atcgggaaca gcgtcgccgg atgtccaatc tcctgcacaa
     4681 tattcgcctg aataccattg cgcgccagcg ctgcgtcaat cagcggtcgg ctgcctgacg
     4741 cgtaatcctg caacaccaat ttcgctccct gtaatgcctg ccagggtaca taatcttcga
     4801 cggccaaagc gctatcgcga tggcaaagca gaaagaaagg ctcggaaaga atcgcttcac
     4861 attgcagatc gcccactggg cctggatcga tgacgatgcc aaagtcgaca tctccctggc
     4921 gaatactttc catcacccac tgctgcggac gatcgtgcag gacaaactga atatctggat
     4981 agcggcgatg gctttcagca atgcattgcg gaataagatg cgcggaaatg gtctggctgg
     5041 cagcgacccg aacttttccg ctcagttgtt gccccatacg cccggtatcg cgcaacgtgc
     5101 tgttcagttc atccagcagt cgctcaagac gcaaagccag ctgctgccct gcatctgtaa
     5161 gcaccacttc acgcgtggtt ctgtccagca ggcgaacacc agtatgattt tccagttcct
     5221 tcacactgtg actcactgcc gactggctca ggccgatacg ctctcctgca cgactaaaac
     5281 ttttctcctg cgctactgtg acgaaaacct ttaattgttt taaagaataa ttcatctgtt
     5341 ttcttcatga atggatgcaa taaatcaatt ttatttctca aactggaaga agcacaatag
     5401 aaccatcgat catctggagt ctttatgaaa ctttttcgta tcctcgatcc tttcacctta
     5461 accctgatca cggtggtgtt gctggcctct ttctttccgg ccagaggcga tttcgtcccc
     5521 ttctttgaaa atctgaccac cgcagcgatt gccctgctgt tctttatgca cggcgcgaag
     5581 ttgtcgcgtg aggcgattat tgctggcggt ggtcactggc gactgcattt gtgggtaatg
     5641 tgcagcacct tcgtgctgtt tccgattctg ggtgtactgt ttgcctggtg gaaaccggta
     5701 aatgtcgacc cgatgctcta ctccggtttt ctctacttgt gcattctccc ggctaccgtg
     5761 cagtctgcaa tcgccttcac gtcaatggcg ggcggtaacg tcgcggcggc ggtttgttct
     5821 gcgtcagcat ccagcctgct ggggattttc ctttcaccat tgctggttgg tctggtgatg
     5881 aatgttcacg gtgcaggggg cagccttgag caggtcggta aaattatgct gcaactgctg
     5941 ctgccgtttg tgttggggca tctttcccgg ccgtggattg gtgactgggt gtcgcgcaat
     6001 aaaaaatgga ttgcgaaaac tgaccagacg tccattctgt tggtggttta tacagcgttc
     6061 agcgaagccg tcgttaatgg tatctggcat aaagttggct ggggatcatt gctgtttatc
     6121 gtggtggtca gctgcgttct tctggctatc gtgattgtag ttaacgtctt tatggcacgc
     6181 cgactgggct tcaataaggc agatgaaatt actatcgtct tttgtggttc gaaaaagagt
     6241 ctggcaaatg gcatcccgat ggcaaacatt ctgttcccca catcggtgat cggtatgatg
     6301 gtgctgcccc tgatgatttt ccatcagatc caattgatgg tctgtgcggt gctggcgcgt
     6361 cgatacaaac gccagaccga acagttacag gcgcagcagg aaagcagcac cgataaagct
     6421 taaagcggac gcttcagggg ctggaccagt tgcgtcagcc cctcggtttt aatcagcagc
     6481 gtgatttgca ttagctcacc gagcttccct gccgggaact catctttgcg ggcaaaccac
     6541 agcagatact cctccggcag gtcgattaag cgacgccctt tgtatttgcc aaacggcatt
     6601 atcgtattgg ctatttcaat cagctgctct ttttccatct cagctaccca gcaaacgcag
     6661 catttccgct tcgtcgatga cttcaatgcc cagttcctgc gccttcgcca gtttagatcc
     6721 tgcagcttca cccgctatca ccagatcggt tttcttcgac acgctgcccg cgactttcgc
     6781 gcccagttcg accagtcgag ctttagcgtc atcacgtgac atctgactta agctgcccgt
     6841 aagcaccacg gttttaccag caaacgggct gtcaatctct tccgcgttga taacgatcgg
     6901 cgcaggccag tggacacctt ccgccaacag ctcgctgata acattgcggt tgctttcttc
     6961 ggcaaagaag ttgtgaacgt gggatgcaac gacaatgcca acatcaggca ccttttgcag
     7021 ctcttcaatc gaagcggctt ccagcgcttc cagcgtgccg aaatatgccg ccagacctgc
     7081 tgcggtggcc tcgccgactt cacggatgcc aagtgcatag aggaagcgag caaaggtggt
     7141 ttctttcgct ttttctagcg cgttaaccac gttttgtgcc gattttggcc ccatacgctc
     7201 cagtccggtc agtttgcctg cggtgagttt gaacagatct gccggagtat ggacatattc
     7261 tttttcaacc agctgatcga tgattttgtc gcccattccg tcaacatcca tcgcacggcg
     7321 ggaaacaaag tgtttcagcg actctttacg ctgcgcaccg caaatcaggc caccggtaca
     7381 gcgggcaacc gcttcacctt ccacacgctc aacgtcagaa ccacataccg gacaatgcgt
     7441 tgggaataca acctcacggg tatcttccgg gcgttcagaa agcacgacgt taaccacctg
     7501 cgggatcacg tcgccagcgc ggcgaatcac cactttatcg ccaatgcgta aaccaagacg
     7561 ttcgatttca tccgcattgt gtaaggttgc gttactcacc agcacgccag caacatggac
     7621 aggttccaga cgcgcaacag gcgtaatcgc gccagtacgc ccaacctgaa actcgacgtc
     7681 acgcacaaag gtcatctgct cctgcgccgg gaatttaaac gctaccgccc agcgcggggc
     7741 acgcgcgaca aagccaagct gctcctgctg ttccagtgag ttgaccttaa tcaccacgcc
     7801 gtcgatatca aagcccagcg tcgggcggtc ttcttccact ttgtgataga acgccagcac
     7861 ttcttccgcc gattcacaaa gcgttacccg atcgctgacc ggcaaccccc actttttaaa
     7921 ttgcagtaaa cggccaagat gagtatccgg cagctcgcca ccttccagaa caccaacgcc
     7981 atagcagaaa aaagtgagcg gtcgcttcgc tgtaatacgc ggatcaagct gacgaagtga
     8041 accagctgcc gcattacgtg ggttagcaaa aactttcccg cccgtgcgtc gcgcatcttc
     8101 gttaatcttt tcgaaccccg cctgcggcag gaacacttca ccacgcactt ccagacgcgc
     8161 cgggatattc tctccgtgca gcttcagcgg aatagcgcga atagtacgca cattagacgt
     8221 gatatcttcc ccggtggtgc catcgccacg ggtcgcggca ctgactaaaa cgccattttc
     8281 atacagaata ctgacggcaa ggccatccag cttcagctca cagcaccagg tgactttctc
     8341 gttgtttttc agacggtcct gcacacgttt gttgaaagca agaaagcttt cttcatcaaa
     8401 aacgttatcc agtgacagca ttggtacttc atggcgtatc tggctgaaag ccgccagcgg
     8461 cgcagcacct acacgttggg taggcgaatc aggcgtaatc agttctggat gtttggtttc
     8521 cagctcgcgc agttcgcgca tcagcctgtc gtattcagcg tcgggaattt ccggcgcatc
     8581 catcacatga taaagatatt catgatggcg aagcgtcgtt cgcagttctg tcagttgttg
     8641 ttcgattgat tccatatcgc accatcaatg ctaaaaaccc ccgacaagcg ggggttcgaa
     8701 gaggagttaa tttgccttaa gtgtatcagg cgttggcgtc tttgacttcg cggatgatgt
     8761 cctggtactc gcgcaatttc tgcggagtca tcatacggcg ctgatcgtca agcacgacac
     8821 cgcccacttc atcggcaata tgctgcgcag attgcagcat cagcttgaag ttctgcagct
     8881 cgtcaccgta agacggtacc tgcataaaga tggtgacacc cggagtagtg aaatccttca
     8941 tttcaggatc aaaggttccc ggtttcacca tattcgccag gctgaataac gccgggccgc
     9001 tgccatccgg gctaagatga cgatggtaaa tattcatatc gccaaaaatg aagcccgctt
     9061 gttgaatgct gttaagaagc agttcaccgt ttagctcgct accgtgatgc gccgcgacgt
     9121 tcatgataat caccgcttct ttgcgcttcg gtttatccat aactggagcc ggttccgcta
     9181 caggctcagg ctgtggtgcc gctacgggtt ctgcaggctg gaaagcctgt tgtgccggtt
     9241 gcggtgctga atgcacaggc tgcggcgctg gcgcgacctg tggcgaaact ggctgctgca
     9301 acggctgttc aggctgcggc tgataggcag gctgctgcac cggctgcgct ggacgcggag
     9361 catgttgcgg cggtacctgc gcttcaggcg gctgctggac cggttgacgc ggctgcgcag
     9421 acgcataagg cggttggtac tggtgttgcg gcgacggacg agcagcctca tgctcctgag
     9481 cgttagccgg ggcatgattc acgcggtgaa cacgaacctc accaacgccc tcatcatctt
     9541 cgacatcctc gtcataagaa tcgtcgtcac gttttgactt cattcgtttt aatggccgat
     9601 cgcggaacat agaagatcgt tctttacggc tggtccagaa accatgtacc agtaaagcga
     9661 ttatggcgat cgcgccaaca atgattaata tcagacgcaa atcctgcatc attatattct
     9721 ctgttgttct aacaccttgc caccacggca aacatttact cactaagagt atttgccgat
     9781 tacctcaagt gcaagtgcac tattaacttt cacagcacaa agatagatga aatcgtgctt
     9841 tttgctgttt tttcgaacat atcctaactg tccattgcgc aattacccgg taaaatacgc
     9901 agaattttcc tgggattggt caaaaggagc tcatcctgat tatggtttca tcattcacat
     9961 ctgccccacg cagcggtttt tactattttg cgcaaggctg gaagctcgtc tcgcaacctg
    10021 ggattcggcg tttcgttatt ttaccgctgc tggtcaatat tttgttgatg gggggcgcat
    10081 tctggtggct ctttacacag ctcgatgtct ggatcccgac tctcatgagt tacgttccgg
    10141 actggctaca atggctgagt tatctgttgt ggcctctggc tgtcatctct gtgctgttag
    10201 tgtttggcta tttcttctcc acgattgcta actggattgc cgctccgttt aacggtttat
    10261 tggctgaaca actggaagca cgattgactg gcgctacacc gccagatacc gggattttcg
    10321 gtatcatgaa agatgtgccg cgaatcatga aacgcgaatg gcaaaaattt gcctggtatc
    10381 tgccgcgcgc aattgtattg ctaattcttt acttaatccc tggtattggg caaaccgtcg
    10441 cgccggtact gtggttcctg tttagcgcct ggatgttagc catccagtat tgcgattacc
    10501 ccttcgataa ccacaaagtg ccgtttaaag agatgcgcac cgccctgcgc acacgcaaaa
    10561 tcaccaatat gcagtttggt gctttaacca gcctgtttac gatgatcccg ctgcttaatc
    10621 tgttcatcat gcccgttgcc gtttgtggcg cgacggcgat gtgggtcgat tgctatcgcg
    10681 ataaacacgc gatgtggcgg taacaatcta ccggttattt tgtaaaccgt ttgtgtgaaa
    10741 caggggtggc ttatgccgcc ccttattcca tcttgcatgt cattatttcc cttctgtata
    10801 tagatatgct aaatccttac ttccgcatat tctctgagcg ggtatgctac ctgttgtatc
    10861 ccaatttcat acagttaagg acaggccatg agtaagattt ttgaagataa ctcgctgact
    10921 atcggtcaca cgccgctggt tcgcctgaat cgcatcggta acggacgcat tctggcgaag
    10981 gtggaatctc gtaaccccag cttcagcgtt aagtgccgta tcggtgccaa catgatttgg
    11041 gatgccgaaa agcgcggcgt gctgaaacca ggcgttgaac tggttgaacc gaccagcggt
    11101 aataccggga ttgcactggc ctatgtagct gccgctcgcg gttacaaact caccctgacc
    11161 atgccagaaa ccatgagtat tgaacgccgc aagctgctga aagcgttagg tgcaaacctg
    11221 gtgctgacgg aaggtgctaa aggcatgaaa ggcgcaatcc aaaaagcaga agaaattgtc
    11281 gccagcaatc cagagaaata cctgctgctg caacaattca gcaatccggc aaaccctgaa
    11341 attcacgaaa aaaccaccgg tccggagatc tgggaagata ccgacggtca ggttgatgta
    11401 tttattgctg gcgttgggac tggcggtacg ctgactggcg tcagccgcta cattaaaggc
    11461 accaaaggca agaccgatct tatctctgtc gccgttgagc caaccgattc tccagttatc
    11521 gcccaggcac tggctggtga agagattaaa cctggcccgc ataaaattca gggtattggc
    11581 gctggtttta tcccagctaa cctcgatctc aagctggtcg ataaagtcat tggcatcacc
    11641 aatgaagaag cgatttctac cgcacgtcgt ctgatggaag aagaaggtat tcttgcaggt
    11701 atctcttctg gagcagctgt tgctgcggcg ttgaagctac aagaagatga aagctttacc
    11761 aacaagaata ttgtggttat tctaccgtca tcgggtgagc gttatttaag caccgcattg
    11821 tttgccgatc tcttcactga gaaagaactg caacagtaat gccagcttgt taaaaatgcg
    11881 taaaaaagca cctttttagg tgcttttttg tggcctgctt caaacttt
//
LOCUS       DAMZET010000130        11869 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.130, whole genome shotgun sequence.
ACCESSION   DAMZET010000130 DAMZET010000000
VERSION     DAMZET010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.130"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(26..1006)
                     /gene="ygfZ"
                     /locus_tag="POB34_003531"
     CDS             complement(26..1006)
                     /gene="ygfZ"
                     /locus_tag="POB34_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HDI5920416.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFCELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            1249..1515
                     /gene="sdhE"
                     /locus_tag="POB34_003532"
     CDS             1249..1515
                     /gene="sdhE"
                     /locus_tag="POB34_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HDI5920417.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            1496..1903
                     /locus_tag="POB34_003533"
     CDS             1496..1903
                     /locus_tag="POB34_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HDI5920418.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(1943..2464)
                     /gene="fldB"
                     /locus_tag="POB34_003534"
     CDS             complement(1943..2464)
                     /gene="fldB"
                     /locus_tag="POB34_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HDI5920419.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            2576..3472
                     /gene="xerD"
                     /locus_tag="POB34_003535"
     CDS             2576..3472
                     /gene="xerD"
                     /locus_tag="POB34_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDI5920420.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            3497..4207
                     /gene="dsbC"
                     /locus_tag="POB34_003536"
     CDS             3497..4207
                     /gene="dsbC"
                     /locus_tag="POB34_003536"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HDI5920421.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            4213..5946
                     /gene="recJ"
                     /locus_tag="POB34_003537"
     CDS             4213..5946
                     /gene="recJ"
                     /locus_tag="POB34_003537"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDI5920422.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMILKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            6037..7135
                     /gene="prfB"
                     /locus_tag="POB34_003538"
     CDS             join(6037..6111,6113..7135)
                     /gene="prfB"
                     /locus_tag="POB34_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDI5920423.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            7145..8662
                     /gene="lysS"
                     /locus_tag="POB34_003539"
     CDS             7145..8662
                     /gene="lysS"
                     /locus_tag="POB34_003539"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDI5920424.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(8705..9253)
                     /gene="idi"
                     /locus_tag="POB34_003540"
     CDS             complement(8705..9253)
                     /gene="idi"
                     /locus_tag="POB34_003540"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDI5920425.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            9308..9379
                     /locus_tag="POB34_003541"
     CDS             9308..9379
                     /locus_tag="POB34_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920426.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKSI"
     gene            complement(9376..9501)
                     /locus_tag="POB34_003542"
     CDS             complement(9376..9501)
                     /locus_tag="POB34_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920427.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(9503..10951)
                     /gene="uacT"
                     /locus_tag="POB34_003543"
     CDS             complement(9503..10951)
                     /gene="uacT"
                     /locus_tag="POB34_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HDI5920428.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            11387..>11869
                     /locus_tag="POB34_003544"
     CDS             11387..>11869
                     /locus_tag="POB34_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDI5920429.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     V"
BASE COUNT         3024 a         2936 c         3151 g         2758 t
ORIGIN      
        1 taaatgcctg atgcgataaa gagatttact cttcgagcga atacggcagc ggctcgatat
       61 gcagtgtatt cgcatcgtcg cgtacgcgga agatgctatc cggttccata tcgttattca
      121 tgacgacctg taccacgacc tgaccatctt ccagttttac cgcagccagc accgtaccgg
      181 tacgacgcca gttctcgccc attttcagct ctaagtcttc accagcttcc ggcagtcggc
      241 tggcgctacc tgccagcaac cagagcgcac gtttattggc accacggaat tttgctcgcg
      301 ccaccatctc ttgtccggta tagcagcctt ttttaaagct gataccgccc agcgcctgga
      361 ggttggtcgc ctgtgggata aactgcccgc tgttggcggc atcaatcacc gggaaacccg
      421 cttcaatgtt taatgccagc cactgttggc tattgttcag ttccgcttca ccgcgcagtt
      481 tatcggtcag catattagca gtagcttcat cggttacgat caggaaacgt tctgccgggt
      541 gttcaaacca tagcaaagtg gtcgcgcctt ctttaactac ctgtttttct ttcgaaggca
      601 gttcacaaaa gagatttgcc agcgcggcgc gcgcctgaaa accggcaaca ccaagcagca
      661 cacgctcgtc gtctggcgcg atggtcactt tagagaatac cgcatatttt ttcagttcag
      721 tcagctgcgg ttcacgcacg ctgcgccgtt caatccatgc aaagccatcg ccgtcgcgga
      781 acagacgtaa attgctccac attttacctt tggcgtcgca atgggcggcg agcaggtgct
      841 gatcttctgc catctggctg acatctgctg tcacctgacc ctgcatatat ttttcgctgt
      901 ccgcgccagt aatggtggca agcgcccagt catcaagcgt catcagcgtc agcggtaaac
      961 gggcagaagc cgtaggctga cggggaggaa aaggtgtaaa agccataata gagtcctgag
     1021 ttgcttaacg gcgtattaat gactaatggt aaaagagcta tgaggcaatg caagtgcttt
     1081 cactacgcga tctgtgcctt ctgcgaggcg acttccaaaa aaatgaagcc attgaattgt
     1141 tgtgaattaa tttcgcgatc gcgatggtgt ttactgatat tcccgtaaaa gaaggcgaaa
     1201 aacacgttac aatagcgggg tactggattt tcgtgagaca ggaagaagat ggacattaac
     1261 aacaaagccc gcattcattg ggcatgccgc cgtggtatgc gcgaactcga tatttcaatc
     1321 atgccgtttt tcgaacatga gtacgacagc ttaagcgatg acgaaaaacg catctttatt
     1381 cgtctgctgg aatgtgacga tccggacctg tttaactggc tgatgaatca cggtaaaccg
     1441 gccgatgcag aactggaaat gatggtccga ctcatccaga cacggaaccg ggaacgtggt
     1501 cctgtggcaa tctgatttgc gcgtctcctg gcgcgcacag tggctttcct tgctgattca
     1561 tgggctggtt gccgctgtta ttttactcat gccctggcca ctcagttaca ccccgttatg
     1621 gatggtgtta ctttcgctgg tggtgtttga ttgcgttcgc agccagcggc gtattaatgc
     1681 tcgccagggg gaaattcgct tgttgatgga cgggcgtttg cgttggcaag ggcaggagtg
     1741 gagcatcgtc aaagcaccgt ggatgattaa gagcggcatg atgctgcgtt tacgttctga
     1801 tggcggtaaa cggcaacatt tatggctggc agccgacagc atggacgaag ccgaatggcg
     1861 ggatttacgg cggattttgt tgcaacaaga gacgcaaaga taaagatgac ggcaaagcat
     1921 atgcgctaag ccgtcagtga gatcaggcgt aatgctctgc catttcgttg aggatttgct
     1981 cgcaccagct ctgaatacgc tcgtcgctaa ggtcatactg gttagtttca tccagcgcca
     2041 gacccacgaa cagttgcccg tcagcaatca ccggtttcgg gctggtaaat tcatatcctt
     2101 ccgttggcca gtagccgacg aacttcacgc ctttggtcga gagtttgtca tgcagcatac
     2161 cgagcgcatc gaggaaccac tcgccgtatc ccagttgatc gccaagccca tacagcgcaa
     2221 caattttacc ttcaaggttc aggtcgtcga gctgatccca gacggcttcc cagtcttctt
     2281 ggatttcacc aaaatcccag gtcgggatac ccagaatgag cacatcgtac tgctccatta
     2341 atttcgggga gtcgtccttg aggttatgta aggtcaccag ttctgggccg ataatatcgc
     2401 ggattttttc tgccgccatt tcggtgtaac aggtgctgga accgtaaaaa agacccatat
     2461 tcataacgta agcttctcga ttctgctgtg gcttttgcgg ctagtgtatc agaatcgctt
     2521 tagtcacggc acaataaggc ataatggatc aaatgagtga ccataagggg cgcaagtgaa
     2581 acaggatctg gcacgcatcg agcagtttct tgatgctctg tggctggaaa aaaatctggc
     2641 tgaaaatacg ttgaacgctt accgtcgcga tctgtcaatg atggtggagt ggttgcatca
     2701 ccgcgggttg acgctggcga cggcgcaaag tgacgatttg caggcattac tggcagaacg
     2761 gctggagggc gggtataaag ccaccagctc agcgcgtttg ctgagtgcag tgcgccgatt
     2821 gttccagtat ctttatcgcg aaaagtttcg tgaagacgat cccagtgcgc atctcgcttc
     2881 accgaaattg ccccagcgtt tgccaaaaga tttaagcgaa gcgcaggttg aacgtttatt
     2941 acaggcacca ttaattgatc agccactgga gctacgcgat aaagccatgc ttgaagtgtt
     3001 gtatgctacc gggctgcgtg tctctgaact ggtcggactg acaatgagtg atatcagcct
     3061 gcgtcagggc gtagtgcggg tcattggtaa aggcaacaaa gagcgcctgg taccgttagg
     3121 tgaagaggcg gtttactggc tggaaaccta tctggaacat gggcgtccgt ggctgctgaa
     3181 tggtgtgtca attgacgtgt tgtttcccag ccagcgtgcg cagcagatga cgcgacagac
     3241 cttctggcac cgcattaaac attatgctgt gctggcgggt atcgacagcg aaaagctgtc
     3301 accgcatgtg ttgcgtcacg cttttgccac ccatttatta aatcatggtg cggatttacg
     3361 cgtggtgcag atgctactgg gccacagcga cctctccacc acgcaaattt atacgcatgt
     3421 cgctaccgag cgtctgcggc aacttcatca acagcatcac ccgcgggcgt gatgtctgaa
     3481 aagaacggga agatttatga agaaaggttt tatgttgttt actttgttag cggcgttttc
     3541 aggttttgct caggctgatg acgcggcaat tcaacaaacg ttagccaaaa tgggcatcaa
     3601 aagcagcgat attcagcccg cgcccgtagc tggcatgaag acagttctga ctaacagcgg
     3661 cgtgttgtac atcaccgatg atggtaaaca tatcattcag gggccaatgt atgacgttag
     3721 cggcacggct ccggtcaatg tcaccaataa gatgctgtta aagcagttga atgcgcttga
     3781 aaaagagatg atcgtttata aagcgccgca ggaaaaacac gtcatcaccg tgtttactga
     3841 tattacctgt ggttactgcc acaaactgca tgagcaaatg gcagactata acgcgctggg
     3901 gatcaccgtg cgttatcttg ctttcccgcg ccaggggctg gacagcgatg cagagaaaga
     3961 aatgaaagct atctggtgtg cgaaagataa aaacaaagcg tttgatgatg tgatggcagg
     4021 taaaagcgtc gcaccagcca gctgcgacgt ggatattgcc gaccattacg cacttggcgt
     4081 ccagcttggc gttagcggta ctccggcagt tgtgctgagc aatggcacac ttgttccggg
     4141 ttaccagccg ccgaaagaga tgaaagaatt cctcgacgaa caccaaaaaa tgaccagcgg
     4201 taaataattc gcgtgaaaca acagatacaa cttcgtcgcc gtgaagtcga tgaaacggca
     4261 gacttgcccg ctgaattgcc tcccttgctg cgccgtttat acgccagccg gggcgtgcgc
     4321 agtgcgcaag aactggaacg cagtgttaaa ggtatgttgc cctggcagca actgagcggc
     4381 gtcgaaaagg ccgttgagat cctttacaac gcttttcgcg aaggaacgcg gattattgtg
     4441 gtcggcgatt ttgacgccga cggcgcgacc agcacggctc taagcgtgct ggcgatgcgc
     4501 tcgcttggtt gcagcaatat cgactatctg gtaccaaacc gtttcgaaga cggttacggc
     4561 ttaagcccgg aagtagtcga tcaggcccat gcccgtggcg cgcagttaat tgtcacggtg
     4621 gataacggta tttcctccca tgcgggcgtt gaacacgctc gctcgttggg cattccggtt
     4681 attgttaccg atcaccattt gccgggcgaa acattacccg cagcggaagc gatcattaac
     4741 cctaacttgc gcgactgtaa tttcccgtcg aaatcactgg caggcgtggg tgtggcgttt
     4801 tatctgatgc tggcgctgcg cacctttttg cgcgatcagg gctggtttga tgagcgtggc
     4861 atcgcaattc ctaacctggc agaactgctg gatctggtag cgctggggac agtagcggac
     4921 gtcgtgccgc tggacgctaa taatcgcatt ctgacctggc aggggatgag tcgcatccgt
     4981 gccggaaagt gccgtccggg gattaaagcg ctgctggaag tggcaaatcg cgatgcgcaa
     5041 aaactcgccg ccagcgattt aggttttgcg ctggggccac gtctcaatgc tgccggacga
     5101 ctggacgata tgtccgtcgg tgtggcgctc ttgctgtgcg acaacatcgg cgaagcgcgc
     5161 gtgctggcaa atgaactcga tgcgctaaac cagacgcgaa aagagatcga acaaggaatg
     5221 caagttgaag ccctgaccct gtgcgagaaa ctggagcgaa gtcgcgacac gctacccggc
     5281 gggctggcaa tgtatcaccc cgaatggcat cagggcgttg tcggtattct ggcttcgcgc
     5341 atcaaagagc gttttcaccg tccggttatc gcctttgcgc cagcaggtga tggtacgctg
     5401 aaaggttcag gtcgctccat tcaggggctg catatgcgtg atgcactgga gcgattagac
     5461 acactctacc ctggcatgat actgaagttt ggcggtcatg cgatggcggc gggtttgtcg
     5521 ctggaagagg ataaattcga actctttcaa caacggtttg gcgaactggt tacagagtgg
     5581 ctggaccctt cgctattgca aggcgaagtg gtgtcagacg gtccgttaag cccggccgaa
     5641 atgaccatgg aagtggcgca gttgctgcgc gatgctggcc cgtgggggca gatgttcccg
     5701 gagccgctgt ttgacggtca tttccgtctg ctgcaacagc ggctggtggg cgaacgtcat
     5761 ttgaaggtga tggtcgaacc ggtcggcggc ggtccactgc tggatggtat tgcttttaat
     5821 gtcgataccg ccctctggcc ggataacggc gtgcgtgaag tgcaactggc ttataagctc
     5881 gatatcaacg agtttcgcgg caaccgcagc ctgcaaatta tcatcgacaa tatctggcca
     5941 atttagcgtc atcttctcta taaaaaagag cgtggattgg gtacaatccc gctcttatca
     6001 ccgcattttg actagctcaa taaaagaaat cagaccatgt ttgaaattaa tccggtaaat
     6061 aatcgcattc aggacctcac ggaacgctcc gacgttctta gggggtatct ttgactacga
     6121 cgccaagaaa gagcgtctgg aagaagtaaa cgccgagctg gaacagccgg atgtctggaa
     6181 cgaacccgaa cgcgcacagg cgctgggtaa agagcgttcc tccctcgaag ccgttgtcga
     6241 caccctcgac caaatgaaac aggggctgga agatgtttct ggtctgctgg aactggctgt
     6301 agaagctgac gacgaagaaa cctttaacga agccgttgct gaactcgacg ccctggaaga
     6361 aaaactggcg cagcttgagt tccgccgtat gttctctggc gaatatgaca gcgccgactg
     6421 ctacctcgat attcaggcgg ggtctggcgg tacggaagca caggactggg cgagcatgct
     6481 tgagcgtatg tatctgcgct gggctgaatc gcgtggtttc aaaactgaaa tcatcgaaga
     6541 gtcggaaggt gaagtggcgg gtattaaatc cgtgacgatc aaaatctccg gcgattacgc
     6601 ttacggctgg ctgcgtacag aaactggcgt tcaccgcctg gtgcgtaaga gcccgtttga
     6661 ctccggcggt cgtcgccaca catcgttcag ctctgcgttt gtttacccgg aagttgatga
     6721 tgatattgat atcgaaatca acccggcgga tttgcgcatt gacgtttatc gcgcgtccgg
     6781 cgcgggcggt cagcacgtta accgtaccga atctgcggtg cgtattaccc acatcccaac
     6841 cgggatcgtg acccaatgcc agaacgaccg ttcccagcac aagaacaaag accaggccat
     6901 gaagcagatg aaagcgaagc tttatgaact ggagatgcag aagaaaaatg ccgagaaaca
     6961 ggcgatggaa gataacaaat ccgacatcgg ctggggcagc cagattcgtt cttatgtcct
     7021 tgatgactcc cgcattaaag atctgcgtac cggggtagaa acccgcaaca cgcaggccgt
     7081 gctggacggc agcctggatc aatttatcga agcaagtttg aaagcagggt tatgaggaac
     7141 caacatgtct gaacaacacg cacagggcgc tgacgcggta gtcgatctta acaatgaact
     7201 gaaaacgcgt cgtgagaagc tggcgaacct gcgcgagcag gggattgcct tcccgaacga
     7261 tttccgtcgc gatcatacct ctgaccaatt gcacgcagaa ttcgacggta aagagaacga
     7321 agaactggaa gcgctgaaca tcgaagtcgc cgttgctggc cgcatgatga cccgccgtat
     7381 tatgggtaaa gcgtctttcg ttaccctgca ggatgtgggt ggccgcattc agctgtacgt
     7441 tgcccgtgac gatctgccgg aaggcgttta caacgagcag ttcaaaaaat gggacctcgg
     7501 tgacatcctc ggcgcgaaag gtaaactgtt caaaaccaaa accggcgaac tgtctatcca
     7561 ctgtaccgag ctgcgtctgc tgaccaaagc actgcgtccg ctgccggata aattccacgg
     7621 cttgcaggat caggaagcgc gctatcgtca gcgttatctg gatctcatct ctaacgatga
     7681 atcccgtaac acctttaaag tgcgctcgca gatcctctct ggtattcgcc agttcatggt
     7741 gaaccgcggc tttatggaag ttgaaacgcc gatgatgcag gtgatccctg gcggtgccgc
     7801 tgcgcgtccg tttatcaccc accataacgc gctggatctc gacatgtacc tgcgtatcgc
     7861 gccggaactg tacctcaagc gtctggtggt cggtggcttc gagcgtgtat tcgaaatcaa
     7921 ccgtaacttc cgtaacgaag gtatctccgt acgtcataac ccagagttca ccatgatgga
     7981 actctacatg gcttacgcag attacaaaga tctgatcgag ctgaccgaat cgctgttccg
     8041 tactctggca caggatattc tcggtaagac ggaagtgacc tatggcgacg tgacgctgga
     8101 cttcggtaaa ccgttcgaaa aactgaccat gcgtgaagcg atcaagaaat atcgcccgga
     8161 aaccgacatg gcggatctgg ataacttcga ctctgcgaaa gcgattgctg aatctatcgg
     8221 tatccacgtt gagaagagct ggggtctggg ccgtatcgtt accgagatct tcgaagaagt
     8281 ggcagaagca catctgattc agccgacctt cattactgaa tatccggcag aagtttctcc
     8341 gctggcgcgt cgtaacgaca ttaacccgga aatcacagac cgctttgagt tcttcatcgg
     8401 tggtcgtgaa atcggtaacg gctttagcga gctgaatgac gcggaagatc aggcgcaacg
     8461 cttcctggat caggttgccg cgaaagacgc aggtgacgac gaagcgatgt tctacgatga
     8521 agattacgtt accgcactgg aacatggctt accgccgaca gcaggtctgg ggattggcat
     8581 cgaccgtatg gtaatgctgt tcaccaacag ccataccatc cgcgacgtta ttctgttccc
     8641 ggcgatgcgt ccggtgaaat aagcattacg ttatgctcac aaccccggca aatgtcgggg
     8701 ttttttattt aagctgggta aatgcagata atcgtattct ggcttcgcga tttgtcgcct
     8761 gcatcaccat ccacggactg aacgcccacg gcgtggcatc aataccgtgt aatacatcag
     8821 ctaaatcaca ccattgataa tccatcactt catcatcgtt gatctgtaac gcactggtgg
     8881 tgcgtgcggc aaataccgga cacacttcat tttccacaat gccactcgga tcggtggcgc
     8941 ggtagcgaaa gtcaggatag atagattcag gaggcgtaat ttccacgcca agctcataac
     9001 ggcaacggcg gatcaccgcg tcttcgctgc tttctcccag ttgtgggtgc ccacaaaccg
     9061 agttagtcca cacgccaggc cacgcttttt tgctcagtgc gcggcgggta actaataatt
     9121 gccccttggc attaaacagc caactggaga acgcgagatg taagagggtg tctgccgtgt
     9181 gtgcggcata cttttccagc gtaccagtgg gaactccctg tgcattcaat aaaatgacgt
     9241 gttccgtttg cataatttct cacatgtaat tctgatcact catcattata gataattgaa
     9301 gagtgaaatg attaaccaag tgagcgttta tcgacaaccg cccgttttga gcggatgccg
     9361 acaggtaaaa tccatttaaa aaagtagcat catatatttg aagcgacttt caatggccat
     9421 tgcaacacag tcagaaatga caggaataga gagagtaaaa agcaacagca gactgaatat
     9481 agcgcgcatt aaaaaattca tattattctc catgctcatt tttcttcagc ttgcgcatca
     9541 gaagccacat acgtactgtc ctgactttta aatctttatc agagacggat tccttcacca
     9601 ggtcagcatg atgctggtag ccattaaaga agacattcag cacaacggcg ctaagggttg
     9661 cgagcataat gccgctatgc agcaacggtt gcagtacggc cggtaactta gaaaagaaat
     9721 cgtgagagag cgtcggagtc atgccaacgc cgagactgat cgccacaata tagaggttgt
     9781 aacggttggt ggtgtagtta cagcgcgaca gaattcgaat ccctgtcgcc agtaccatgc
     9841 cgaacatcac cagcccagca ccgcccagca caaattgcgg aatggaggcg accagcaccg
     9901 ccatttttgg caccatgcca aacaggatta atataattcc cgaagaaata cacacccagc
     9961 ggctatgaac gcgcgtcacg ctaaccaggc caacgttttg tgaaaatgac gtgtggggga
    10021 agctgttaaa cgttccgcct atcattgtcc ctacgccatc gacacgcagc ccgcgaataa
    10081 tatcgtgtga agagagttta cgaccgacta tttcacccag tgccaggaac atccccatcg
    10141 actcgataaa cacgatgatt aacacggcag tcatggtcag aatggaaacg ggatcgaaaa
    10201 tcggcatacc aaatgacatc ggcgtgacaa tcgcaaacca tgaagcatca tgtagcccgg
    10261 ataaattgac ttcattcatc atccacgaaa gtaaaaagcc aaatacaatc cccagtaata
    10321 cggcgacgtt ggacataaat cctttcgcat agcgagtaat gagcaagata aaaattaaga
    10381 cagcaaagga gatacctaaa taaacgggat taccatattg cggattccct ttacctccgg
    10441 cggcccagtc aatacccacc tgaatgatgc taagcccaat agaagtaata accacaccgg
    10501 taaccagtgg cgggaataaa ggcatcaagc gaccgataag tggcgctaat aatgtggtga
    10561 taaaacccgc ggcgatagtg gcaccaaata tccccagcag gccgatatcc gggttcatcc
    10621 caatggctat cattggtgtt acagcagcaa aggtcaccga cataatcacc ggcaggcgga
    10681 tccccataaa gcggccgata ccgatacatt gcaataatgt gacgatcccg cagcaaaaga
    10741 gatccgagct aatgagcatc gcaatagcat cttttgagag gcccagtcgg tcaccaatca
    10801 taagaggaac agcgactgca cctgcgtaca tgacaaggac gtgttgcaga ccgagtatga
    10861 tcagctttcc tggtgataat atgcggtcaa cctcatcagt tgggcggtct tgcccagaag
    10921 atgagggaag ttgggaatct atggcgctca tcagtgtttc tcctttcgcc cgccagagta
    10981 aaaagcaacc gtttacgtta atgaaactgt ctaaacacat gctcgtggag tgccagtttt
    11041 tgcgaggaag gggagttaga gacaggaatt gcggaggcaa tcacataatc gtgcttaaac
    11101 cacgaaaatg acaagagaac ctgacaatgg aattatctgt aattttctca tattgatatt
    11161 tcattaatca ttctggcatg tcgcactctc gcatttaatc gtttttatct ggatagcgct
    11221 cttttggtcg gcaatgttga aataatgaaa gcggcataga ttgaggggca ataatttgaa
    11281 ctcagtcgca aaattataat taaaacggtc atgctaccta atatatgccc gtttattgca
    11341 tcgcgaaatt tgagaagaca taactcatta aatgaaaggg atgcaaatga ataagtttat
    11401 cgctgctgaa gctgcggaat gtataggctg tcatgcttgt gaaattgcct gtgcggtggc
    11461 acacaatcaa gaaaactggc cgctgagtca cagtgacttt cgaccgcgta tccacgttgt
    11521 agggaaaggc caggctgcga atccggtggc ctgccatcac tgcaacaatg ccccttgcgt
    11581 tacggcttgt ccggttaatg ctctgacttt ccagtccgat agcgtacaac tggacgagca
    11641 aaaatgtatt ggctgtaaaa gatgcgcaat cgcttgcccc tttggcgtcg ttgagatggt
    11701 cgatacgatt gcgcagaaat gcgacctttg taaccagcgc agttctggca cgcaagcctg
    11761 tattgaagtc tgcccaacac aggcgttacg actgatggac gataaagggt tacagcagat
    11821 aaaggtggcc cgccagcgca aaacggcagc aggaaaagcg tcatcagtc
//
LOCUS       DAMZET010000131        11679 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.131, whole genome shotgun sequence.
ACCESSION   DAMZET010000131 DAMZET010000000
VERSION     DAMZET010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.131"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(66..1580)
                     /gene="araG"
                     /locus_tag="POB34_003545"
     CDS             complement(66..1580)
                     /gene="araG"
                     /locus_tag="POB34_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HDI5920430.1"
                     /translation="MQQSTPYLSFRGIGKTFPAVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(1650..2639)
                     /gene="araF"
                     /locus_tag="POB34_003546"
     CDS             complement(1650..2639)
                     /gene="araF"
                     /locus_tag="POB34_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HDI5920431.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            3436..3939
                     /locus_tag="POB34_003547"
     CDS             3436..3939
                     /locus_tag="POB34_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HDI5920432.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(4018..4269)
                     /locus_tag="POB34_003548"
     CDS             complement(4018..4269)
                     /locus_tag="POB34_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HDI5920433.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(4384..4470)
                     /gene="azuC"
                     /locus_tag="POB34_003549"
     CDS             complement(4384..4470)
                     /gene="azuC"
                     /locus_tag="POB34_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HDI5920434.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            4733..5056
                     /locus_tag="POB34_003550"
     CDS             4733..5056
                     /locus_tag="POB34_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HDI5920435.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            5227..5724
                     /gene="ftnA"
                     /locus_tag="POB34_003551"
     CDS             5227..5724
                     /gene="ftnA"
                     /locus_tag="POB34_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HDI5920436.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(5762..6001)
                     /locus_tag="POB34_003552"
     CDS             complement(5762..6001)
                     /locus_tag="POB34_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HDI5920437.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            6192..7403
                     /gene="tyrP"
                     /locus_tag="POB34_003553"
     CDS             6192..7403
                     /gene="tyrP"
                     /locus_tag="POB34_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HDI5920438.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(7465..8130)
                     /gene="yecA"
                     /locus_tag="POB34_003554"
     CDS             complement(7465..8130)
                     /gene="yecA"
                     /locus_tag="POB34_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HDI5920439.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(8326..8412)
                     /locus_tag="POB34_003555"
     tRNA            complement(8326..8412)
                     /locus_tag="POB34_003555"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8376..8378),aa:Leu,seq:taa)
     gene            complement(8425..8498)
                     /locus_tag="POB34_003556"
     tRNA            complement(8425..8498)
                     /locus_tag="POB34_003556"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8464..8466),aa:Cys,seq:gca)
     gene            complement(8553..8628)
                     /locus_tag="POB34_003557"
     tRNA            complement(8553..8628)
                     /locus_tag="POB34_003557"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8593..8595),aa:Gly,seq:gcc)
     gene            complement(8780..9328)
                     /gene="pgsA"
                     /locus_tag="POB34_003558"
     CDS             complement(8780..9328)
                     /gene="pgsA"
                     /locus_tag="POB34_003558"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDI5920440.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(9385..11217)
                     /gene="uvrC"
                     /locus_tag="POB34_003559"
     CDS             complement(9385..11217)
                     /gene="uvrC"
                     /locus_tag="POB34_003559"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDI5920441.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(11214..>11679)
                     /gene="uvrY"
                     /locus_tag="POB34_003560"
     CDS             complement(11214..>11679)
                     /gene="uvrY"
                     /locus_tag="POB34_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HDI5920442.1"
                     /translation="ATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYLSKGAAP
                     QEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLMITKGQK
                     VNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ"
BASE COUNT         2889 a         2985 c         2710 g         3095 t
ORIGIN      
        1 gctgaatgac gacttaggtg cgccagaccc cgatgtagaa acagaagaca tcatactctc
       61 cttactcagg caactgcctg gctgacttta ggcatcgcaa ggctcagtgc ctgacgctca
      121 tctgcctgct cgtgtaacaa ttcaccggcg atttcacctt cccgcatcac cacaatccgg
      181 tcggcaacgc cgaggacttc aggtaagtcg ctggaggcaa acagcaccgc cacaccctgc
      241 gccgccagcg cataaatcac gttgtaaatt tcatgcttag caccaacatc aatgccgcgc
      301 gtaggttcat ccagcaaaat gaccttcatc tcttccgata accagcggcc cagaatggct
      361 ttttgctgat ttccgcctga gagattcatg atcagttgct cagcgcccgg cgttttgatg
      421 ttgagcgaac gaatgtggtg atcggcattg ttttcttccc aaccgttgtt gattacacaa
      481 ccgccgagca catgtttacg tctggcactg atgttgatat tgtcgcgaac ggagtgcacg
      541 ggaataatgc cttccgcttt gcgatcttcc gggcagagca tcatgcctgc ggcaatggcg
      601 tggctcggtt tacgaatatc gatcggctgt tggtcgatat aaacctgacc ggcggtgatt
      661 tgcgtcccgc caaacaagcc tttcattaat tcgctacgcc ccgctcctac cagaccaaac
      721 agcccaacaa tttcaccact gcgaaccgcc agacttattg gcgtacgcac gcctggtgct
      781 ttcacagcat caagacgcag gcgctcctcg ccataactac gaggttgcca gccgtagata
      841 tcgccaatgt cgcgcccgac catcgcctgc accagcgcgt cgtggtcaac ctgctgcata
      901 tcggtaaagg ttttgacata acgtccatct ttaaagacag tgatggcatc gctgagggca
      961 aatatttctt ccatacggtg agaaacgtat aagatcaccc gcccctcttt tcgcagttca
     1021 cgaataacgc ggaaaagatt gtcgatttcg cgggcagaaa gggagctggt tggctcatca
     1081 aaggcgataa ttttagcgtt acgcgccagc gccttggcga tttcaaccat ctgccactga
     1141 ccaatggaga gatatttcag cggcgtgtcc gggtcaatat ccataccaag atgtttaagt
     1201 tgtaaacccg cctcataatt cagcaatgag cgattcacaa tgccgccttt atgcggcagc
     1261 tggccgagat agatgttttc cgcgacggtc atttccggca cgagatgcag ttcctggtaa
     1321 ataatcgcca cacccgcatt aagtgctgcg gtcgtgtcgg aaaaggacat ttcctgccca
     1381 ttaatcacta cagaacccgt ggttggcgca tagttgccgc tgaggatttt taagagagtt
     1441 gattttcctg cgccattttc acccatcaac gcatgaacct gaccggcata gcagtcaaaa
     1501 ctaatatccg tcagcgcctt aacggcggga aacgttttac cgatgccgcg aaatgagaga
     1561 tacggggtag actgttgcat aacgtctccg tgaatcactg gtcgttcaca ccccctctgc
     1621 atcatgcaga ggggaatttt tccggcaaat tacttaccgc ctaaaccttt tttctccagt
     1681 tcttctttaa agttgtcacg cgtgatcagc accacgtcgg taacttcggt aaattttggc
     1741 ggttcaacgt cttttgctac ccagttgtaa agcatttcgc tggatttata gccatgtacg
     1801 tccgggcttg gcagcaggga accgtagaag ccggttgcct gtgctttaga cagttcgctc
     1861 accgcatcca caccgttaat gccaatgccg atgatatcgg cagctttaaa gccctgacct
     1921 tccgtcgcgc gtacgccgcc cagcacggtg ctgtcgttca taccgacgat cagccaatgt
     1981 ttaacttccg gatgttgaac cagcattgag ttggcagcgt caaatgcccc cgggatgtcg
     2041 ttagatttgg taggtacctg ataaatttgt ttttccggga atccggccgc tttcagcgca
     2101 tccatagaac ccgttgtacg gcgacgtgcc gtatccagtt cgttggcggt aatcgccatc
     2161 accgcgcttt ctttgacatc ccagccacgt ttctgcatct ctttatacag ttcctgaccc
     2221 tgacgttcgc caattttagt cgccgccatc atcaccagcg gaacggtatc cattggctta
     2281 cctttggcgt taacaaactg gtcatccacg gcaatgactt tcatatcgta gccacgcgct
     2341 ttcgcgacga tggcagagcc gagtttgggg tccggagtac aaataacgaa accttttgcg
     2401 ccactggcag ccaggctgtc gatcgcgttc aatgtttttt cgccatccgg cacggcaatc
     2461 ttaataacct caaaccctaa atccttcccg gctttatcgg caaacttcca ttcggtctgg
     2521 aaccacggct cttccggttg cttcaccagg aaaccgagct tcaggttctc cgccatagcg
     2581 gattgtgaca taacggctgc cagaccaatg gctgccaggg ctttagtaaa tttgtgcatg
     2641 gttctctcca gctttagtgt cgttttgtgt agggcaaaaa cgaatgacat tcgttaaatt
     2701 aatcggaaaa caaagcatta ccttttaact aaaagataag tgactgtgtt gacatagttt
     2761 tagcgagaaa ttaattctcc ataggagagc aatatcacat cgcagaatta cagtgagaac
     2821 gtgcataaat ttagcgggaa aagacataag ggaaagccaa tttgtcagac aaattgtcga
     2881 atgcacagca gattaatcca taagattagc ctggaaatcc ttgttgtctt tggtacccat
     2941 gcgggatgtc ttctttttaa ccagtcaata ggccacatta cctggcgttg agtttttgaa
     3001 atggtgtaat aaccgcaact caaagatgtg gaaaatgcac gtcattcatt tcgccattaa
     3061 ttatcactgt gctcattaat taacagaaca cgtataatga gagccatctc gcaaaaatga
     3121 aaaaacgttt tataaaatca tcacttcatc atgaattcaa attcattgat taatatcaac
     3181 aagatacaaa aagcactatc attaaaattc attgcagtta cattgatttc atcaatgaaa
     3241 tgtaaaaata tataaacttg atgatttaag cattttctta tacccgttca gacgttattc
     3301 ttatttcaga tcatcgtcag aattgactcc acgatcacat ttcggaccgg cagaaaggaa
     3361 ttattctgca aacagtaatt atggtgtttt gatttatctt gcacctctcc acttctggat
     3421 ataaggatat taggtatggc aaccgctgga atgcttctca aactcaactc tcaaatgaac
     3481 cgcgagtttt acgcatccaa tctctacctt cacctgagta actggtgttc tgaacagagt
     3541 ctgaacggca ccgccacttt ccttcgcgcc caggcacaga gtaatgtgac ccaaatgatg
     3601 cgcatgttta actttatgaa gagtgtcggc gctaccccca tcgttaaagc cattgatgtt
     3661 cccggtgaaa aactgaactc tctggaagaa ctgttccaaa aaacgatgga agaatacgag
     3721 caacgttcta gtacgttggc acagttagcc gatgaagcga aagaactgaa tgatgattca
     3781 accgtcaatt tcctgcgcga tctggaaaaa gaacagcagc atgatggtct gttgctgcaa
     3841 accattcttg atgaagtgcg cagtgcgaaa cttgcgggta tgtgccctgt gcagaccgac
     3901 caacatgttc tgaatgtcgt gtcacaccag ctgcattgat catcatcggc gctaatgcat
     3961 tgcgccgatg aaggttttga gaaaccgctg cctcatctgt ttgaagcagc ggttttttta
     4021 atgggattca ccctgtgggg taaacttgag ttcaataagc gcgatggctt tttggattgc
     4081 ccgcatggtg accgggtctg cggcggcggg atggttagta aagtcgatat tcttcagctg
     4141 actggacatt ttttcacgaa cttcaacggg cgcgattaca tcgagaacat ccagaatttg
     4201 tttgataacc aactggcaag caaccacatc agaaaccagt tcctgatcgg cattcagcgg
     4261 ctgggacatc gtaaactcct gatagcattt tgaaagccgt tatagtagcg gcttcacatc
     4321 ttcagcgata gtcacatcca ccgtcatcag gacacaaaaa aacctgccgg agcaggtttt
     4381 ttgttatcgg aacatattgc ctggcggtac gtctttgaac gtcttgcaat agttattgaa
     4441 catacttttc aggattttgc gcagtttcat cgcggcactc cgaccatttg ttatacaggt
     4501 gttattgtct ttgcgcttat aatatgacaa ccatcacaaa aatcaatctt tatgtgatac
     4561 aaatcacata aatacccctt taatgttata aaaatgataa tcaaaaaaca gcccccctat
     4621 ttctgacacc tacagatggc aagaaatagc gcctgccagg cgtcttttcc ggccattgtc
     4681 gcagcgctgt aacgcgtaaa atagtgcttt ctcttactct tctggctgga ccatgagact
     4741 tctgattctg actctttcat taataacgct ggcggggtgt acggttactc gccaagccca
     4801 cgtgagcgaa gttgacgcgg caacaggtat tgtacggttg gtttatgacc aggctttttt
     4861 gcagcatgcc cataccgatc gttatgtcag tcgcgggatc gccgatcgcg catgccagca
     4921 ggaaggctat acccacgcga tcccctttgg tcagccagta ggcaactgta gcctttttgc
     4981 gggttcctta tgtttgaata cggaattcac tttgtcatat cagtgccatc attccgcctt
     5041 ccctgtcttt ctctaaatat tttaatcagc gaggggatct tcgctgatta aataaatagc
     5101 ggtaatgcgt tttattccca ttcgtatttt taataattga agtttatatt ttaccttttg
     5161 caaataataa aataacaaat tatagtgacg ccacagcaac aaatataacc tttgtggagc
     5221 actatcatgc tgaaaccaga aatgattgaa aaacttaatg agcagatgaa cctggaactg
     5281 tactcttcac tgctttatca gcaaatgagc gcctggtgca gctatcatac cttcgaaggt
     5341 gctgccgcgt tcctgcgccg tcacgcccag gaagagatga cgcatatgca gcgtctgttt
     5401 gattacctga ctgataccgg caatttaccg cgtattaata ccgttgaatc tccgtttgct
     5461 gaatattcct cacttgatga attattccag gaaacctata aacacgaaca attaatcacc
     5521 cagaaaatta acgaactggc tcatgctgca atgaccaatc aggactaccc aacatttaat
     5581 ttcctgcaat ggtatgtttc tgagcagcat gaagaagaga aactgttcaa atcgattatt
     5641 gataaattaa gcctggcagg caaaagcggc gaaggtctgt attttatcga caaagaactc
     5701 tctaccctcg acacacaaaa ctaatgctct ccggcggcag gtttactgcc gccgtttcaa
     5761 atcagtgacg gcaaatctta ctctgatcgg tcgaaaaacc gtctttcgaa ggtataaatt
     5821 tgccttttgc tgccagaaac gctaccagct ctcccgctgt catcccttct gccgagcagg
     5881 tgtgaaaacg tgcctgttca ccaaaacgcg ctttaatcgc agcttccaga ctggcatgcg
     5941 tatattgctc gcctgattca atcatcatat ttaacacttc atgaccgtga atagagtcca
     6001 tcgtccctcc tcaaaaaaag cctagcgtag cgattgccgc ttatgaagac tttgcgccag
     6061 cgcaggactg aatgcttttt attgtacatt tatatttaca ccatatgtaa cgtcggtttg
     6121 acgaagcagc cgttatgcct taacctgcgc cgcagatatc actcataaag atcgtcagga
     6181 cagaagaaag cgtgaaaaac agaaccctgg gaagtgtttt tatcgtggcg ggaaccacaa
     6241 ttggcgcagg catgctggca atgccgctgg ctgcggccgg tgttggtttt agcgttacgt
     6301 taatcttgtt gattgggctt tgggcgttga tgtgctacac ggcgctatta ctgctggagg
     6361 tgtaccagca tgttccggca gataccggtc ttggcacgct ggcaaaacgc tatctgggac
     6421 gctacggtca atggctgacg ggcttcagta tgatgttctt aatgtatgct ctgactgcgg
     6481 catacatcag cggtgccggt gaattgttgg cctccagcat cagcgactgg acaggtattt
     6541 ctatgtcggc aaccgctggc gtgctgttgt tcacttttgt tgccggtggc gtggtttgtg
     6601 tcggaacttc actggtcgat ttatttaacc gttttctgtt cagcgccaag attatttttc
     6661 tggtggtaat gctggtacta ctgctgccgc atattcacaa agtgaatctt ttaaccctgc
     6721 cgttgcaaca ggggctggct ctgtctgcaa tcccggtgat ttttacgtcg tttggttttc
     6781 acggtagcgt gccgagtatt gtcagctata tggatggcaa cattcgtaag ctacgctggg
     6841 tgtttataat cggtagtgcg atccccctgg tggcatatat tttctggcag gtggcgacgc
     6901 ttggcagcat tgattcaaca acctttatgg gattgctggc taatcatgct ggattaaacg
     6961 ggctgttaca ggcgttacgc gaaatggtgg cctctccgca tgttgagctg gcagtgcatt
     7021 tatttgctga tttagccctc gccacgtcat ttctaggcgt tgcgttaggc ttatttgatt
     7081 atctggctga tttatttcag cgttcaaata ccgttggtgg acgcttgcaa actggtgcaa
     7141 ttacgtttct gccgccgttg gcgtttgcac tgttttatcc acgaggattt gtgatggcgc
     7201 tgggttacgc cggtgtggcg ctggcggtac tggcattgat tatcccttcg ctgttgacct
     7261 ggcaaagcag aaagcacaat cctcaggcgg gttaccgggt caaaggtggt cgtccggcgc
     7321 tggtggtggt gtttctctgt ggtattgctg tgattggcgt gcaatttttg attgcggcag
     7381 ggttgttacc agaagtgggg tgatcagata gcctcaaatt ccttattggg tgccagaatt
     7441 aacgctgaca cccaatttgg cctcttaatg caggcagcac tgcttaaatt tcttaccact
     7501 accgcacggg caaggatcgt tacgccccgg tttctcttct gctttgatcg gttgctgaac
     7561 agctttttcc tgcggatgcg ccatccagta cgcatgtaga tcaagcgccg ccagtcgaat
     7621 ggcatctacg ctctcttcaa acgcttctgg cgacattttc tctacccgct cgaagttttc
     7681 ctcagtaccg tgcagcgcaa tcgcctccag cgctggtttt aacgaatcgg gcaacgttga
     7741 ccagtcagaa agtgccacgc cccgcatata gccaaagcac cactcctcaa caatcgtcag
     7801 ctcgctgcca tcaacttctc gcaagccgaa taacggctca aactgctccg ggaattcgtt
     7861 cagacgctct gcggtatcgg ccatatgttg aaaagccaga ttcataaagc gcgtcatctc
     7921 tttctctgac gcccagcgcg gcacatagtc agccccaccc cacacggcaa ccagccactg
     7981 ttccggttca atctcttgcg gagaactcaa caccgccgtc aataaaccgt ccagctccgc
     8041 cacatcaagg atggcgtggt cagtgttgta tttggtcaga atatcgtcca gccattccaa
     8101 ctcactttcg tttaacggtc ccgttttcat acgcttttcc ttgtggatct caactcgcca
     8161 gcacctatct tacatgccgg tccgtatcag agatactttt tgagtggctt tgctggtgat
     8221 taaaaattag ggagggtgta acgacaagtt gcaggcacaa aaaaaccacc cgaaggtggt
     8281 ttcacgacac tgcttattgc tttgatttta ttcttatctt tcccatggta cccggagcgg
     8341 gacttgaacc cgcacagcgc gaacgccgag ggattttaaa tcccttgtgt ctaccgattc
     8401 caccatccgg gctcgggaag aaagtggagg cgcgttccgg agtcgaaccg gactagacgg
     8461 atttgcaatc cgctacataa ccgctttgtt aacgcgccaa attcttcagg cctttcagcc
     8521 agacatccgc ttgaggccga tgtcttttaa actggagcgg gaaacgagac tcgaactcgc
     8581 gaccccgacc ttggcaaggt cgtgctctac caactgagct attcccgcat tcatcaagca
     8641 atcagttaat cacttgattt tattatcgtc tggcaatcag tgccgccgtt cgatgcgttg
     8701 cattctactt acctggcgcg atgagtcaac gatatttttc accacttttg atcgtttgct
     8761 gaaaattacg ccgaaacgat cactgatcaa gcaaatctgc acgcgcagcg ctcaaatatt
     8821 gcaacattga ccacagagtc agtaccgcag ccacaaagaa aagtgcaata ccggcgtact
     8881 caacccaaat gttcggacgc cacagcagcc aggccaacgc caccatctgg gcagtggttt
     8941 tcactttccc aatccaggag acagccacgc tactgcgttt acccaactcc gccatccatt
     9001 cgcgtagcgc agaaataata atttcacggg cgatcatcgt tgccgccggt aaggtcaccc
     9061 accagctgtg ataatgctcg gttaccagca ccatggcgat agccacgaga actttatctg
     9121 cgacagggtc aaggaaagca ccaaaccggg tactctggtt ccagcggcgt gccagaaaac
     9181 catcgaacca gtcagtcacc gccgcgacgc agaaaatgag cgcggcggca aacggcgacc
     9241 aggtgacagg cagataaaag accaatacaa agaatgggat aaggatgaca cggaacagtg
     9301 taagcaacgt agggatatta aattgcataa tgacgggtaa ctatctgttg tcagtaagat
     9361 tacccctatg ttgctacaga gacatcaatg tttcaacgac cagaagatct tttctgccag
     9421 accttgcgaa atacccggca cttttgcaat ttcctcgacg ctggcgttac gtaaaccttg
     9481 caaaccgccc atatatttca acaacatttg ccggcgtttt ggcccgacgc cttcaatggt
     9541 ttccagggaa ctggtatttt tgaccttcgc ccgtttttta cggtgcccgc caatcgcgtg
     9601 atcatgtgat tcatcgcgaa tatgctggat aacatgcagc gcgggagaat ctggcggcaa
     9661 actgaatccc tcaccttccg gctcaaagaa cagcgtttcc agcccagcct tacgatctgc
     9721 tcctttggca acgccaagta gcagcggatg atttttatcc catgagacat ccagttcggc
     9781 gaagacattt ttcgcctgcg caagctggcc tttgccgccg tcgataagta tcacatccgg
     9841 gatcttactg tcgtcgatgg ctttaccata acgccgacgc agcacctgat tcatcgccgc
     9901 ataatcatcg cccggcgtga tgccagtaat gttatagcgc cgatactccg cacgcagcgg
     9961 gccgttagca tcaaacacca cacaggaagc gacggtttgt tcacccatgg tatggctgat
    10021 gtcaaagcac tccatccgct tcacttccgg caatttcaac acactggcaa gtgctgtaag
    10081 ccgctggtga acggtagatt gctgcgaaag tttgctggtt aaggccgtcg ccgcattggt
    10141 gcgcgcgagt ttcagataac gcgcccgatc gccgcgaggt ttggtttgaa cattaatctt
    10201 gcgtcccgcc agttctgaaa gggaatcggc gagcagcgtt ttatcgctaa gattaaaatc
    10261 gagcaggatc tcacccggta aggtgcgcat ctggctgcct tgtaaataga actgacctac
    10321 gaaggtttct accacctcgc tcagttccgt accgccaggc actttcggga aatagctgcg
    10381 gctgccgagc actttgccct gacgaatgaa caatacgtgg acacaagcca tgcccgcatc
    10441 gaacgccaca ccaataacgt caaggtcgtc gccggtattg gaaacaaact gtttttcggt
    10501 gacgcgtcgc accgcctgaa tttggtcgcg aatacgtgcc gcttcttcaa actccagatt
    10561 ctggctggca gtttccatac gactaatgag ttgcgtaagc acctgatcat ctttgccaga
    10621 caaaaacagg cgcacatact cgacctgctg agcgtattct tcttcactca ccagtccttc
    10681 aacgcacggt cccagacagc gccctatctg atattgcaga cacggacgcg agcgattgcg
    10741 ataaacacta ttttcgcact ggcgaatggg gaaaatcttt tgcagtagcg ccagtgtttc
    10801 acgtacggca tagccattcg ggaacgggcc gaaatattca cctttggcat gcttcgcacc
    10861 acgatgcatc gccagacgcg ggtgggtatc gccactcagg aagataaaag gataagattt
    10921 atcatcgcgt agcaaaacgt tgtaacgcgg ctgatagagt ttgatgtagt tgtgttccag
    10981 caacagcgct tcggtttctg tatgagtaac cgttacatca atttgctgga tctgggcgac
    11041 cagcgcttcg gttttgcgcg aagcgaggtt gctacggaaa tagctggaaa gccgtttttt
    11101 caggtctttc gctttgccga catagataac cgtaccacca gcatcgtaca tgcgataaac
    11161 gcctggctgg ctggttacgg tttttaaaaa cgcttttgcg tcaaactgat cactcactga
    11221 cttgataatg tctccgcatt acacagacca tggcgaattg ccaggtgagt cagctcaaca
    11281 tcgccatgaa tgtttagttt actgaacata cgatagcggt agctgttcac cgttttcgga
    11341 ctgagattga gctgttctga gatctcattg accttctggc ccttggtgat catcagcata
    11401 atctgcaatt cacgttcaga caaactggca aatgggcttt ctgttttttc tggttcgatc
    11461 tggcttaacg ccatttgttg agcgatgtca gaagcaatgt aacgctgccc tgaatagaca
    11521 gaacgaatcg cactcacgac ttcctgcgga gccgcgcctt tgctgaggta gcccgcagca
    11581 ccggcctgca tgactttcgc tggtaaaggg ttttctgtat ggacggtaag catgatgatt
    11641 ttgacatcag ctgtggaacg cgcgatttta cgcgtcgcc
//
LOCUS       DAMZET010000132        11493 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.132, whole genome shotgun sequence.
ACCESSION   DAMZET010000132 DAMZET010000000
VERSION     DAMZET010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.132"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1259
                     /gene="nrfB"
                     /locus_tag="POB34_003561"
     CDS             <1..1259
                     /gene="nrfB"
                     /locus_tag="POB34_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HDI5920443.1"
                     /translation="SRWIIGIVFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAM
                     LVMLQLLLLLAYESLWPNAWHFLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYY
                     GLMQGLLSVLRLFWGNLINFMANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSL
                     RPLGQILLENQVITEEQLDTALRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAW
                     ESIDAWQIPSSLIAEMPASVALHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGR
                     KVRYVIVLRGQIVTGLRHWYARRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQF
                     LFAEILTTLGHINRSAINVLLLRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQV
                     SMQSLLLKAGLNTEQVAQLESENEGE"
     gene            1246..4218
                     /gene="nfrA"
                     /locus_tag="POB34_003562"
     CDS             1246..4218
                     /gene="nfrA"
                     /locus_tag="POB34_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HDI5920444.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRAQL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVPTRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNTSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SGSFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            4219..5109
                     /locus_tag="POB34_003563"
     CDS             4219..5109
                     /locus_tag="POB34_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HDI5920445.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            5292..6053
                     /gene="envY"
                     /locus_tag="POB34_003564"
     CDS             5292..6053
                     /gene="envY"
                     /locus_tag="POB34_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HDI5920446.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLIPHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            6566..7519
                     /gene="ompT"
                     /locus_tag="POB34_003565"
     CDS             6566..7519
                     /gene="ompT"
                     /locus_tag="POB34_003565"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HDI5920447.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            7706..9190
                     /locus_tag="POB34_003566"
     CDS             7706..9190
                     /locus_tag="POB34_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI5920448.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENIYCPGNSEAEIQ"
     gene            complement(9449..9679)
                     /locus_tag="POB34_003567"
                     /pseudo
     CDS             complement(9449..9679)
                     /locus_tag="POB34_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937477.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            9736..10404
                     /locus_tag="POB34_003568"
     CDS             9736..10404
                     /locus_tag="POB34_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDI5920449.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     INHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            10770..10883
                     /locus_tag="POB34_003569"
     CDS             10770..10883
                     /locus_tag="POB34_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920450.1"
                     /translation="MNSILIITSLLIIFSIFGHALIKLGIGISNNPDKTDV"
     gene            10952..11185
                     /gene="ydfK"
                     /locus_tag="POB34_003570"
     CDS             10952..11185
                     /gene="ydfK"
                     /locus_tag="POB34_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HDI5920451.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVLKDNQNLNGKV"
BASE COUNT         3240 a         2779 c         2606 g         2868 t
ORIGIN      
        1 aatctcgctg gatcatcggc attgttttcc agggctttaa aacccacaaa tggacctcca
       61 gcctgacgct gaactacttt ctctggcgcg accgcaaagg ggcaatcagt aactttgtca
      121 gcttcctcgc gatgctggtg atgctccagc ttttgctgtt gctggcgtat gaaagtttgt
      181 ggcccaatgc ctggcatttc ctttctattt ttagcggcag cgcatggtta atgaccctgc
      241 tgtggctaaa ctttggcttg atggttaacc gcatcgtgca gcgggtgatt ttcgtcactg
      301 gctactacgg tctgatgcag gggctgctat ctgtcctgcg tcttttctgg ggcaacctga
      361 ttaacttcat ggccaactgg cgcgcgctaa aacaggtact tcaacacggc gatccacgtc
      421 gcgtcgcgtg ggataaaaca acgcatgact tccccagcgt gactggcgat acccgctcgt
      481 tgcgcccgtt aggccaaatt ctgctggaaa atcaggtcat cactgaagaa caactcgata
      541 cagcactgcg taatcgcgtc gaaggtctac gcctgggcgg ttcaatgctg atgcaggggc
      601 tgattagcgc cgagcagctg gcacaggcgc tggcagagca aaacggcgtg gcgtgggaat
      661 ccatcgatgc ctggcagatc ccgtcctcgc tgattgccga aatgccagcc tccgtagcgc
      721 tgcattatgc agtactgccg ctgcgtctgg aaaatgatga gttaattgtc ggcagtgaag
      781 atggtattga cccggtttcg ctggcggccc tgacgcgtaa agtcggacgc aaagtgcgtt
      841 acgtcattgt tctgcgggga caaattgtca cggggttacg tcactggtat gcacgccgac
      901 gcggtcacga tccgcgggca atgttgtaca atgcggttca gcatcagtgg ctcacggaac
      961 agcagaccgg tgaaatctgg cggcaatatg tgccgcatca gttcctgttc gccgaaatac
     1021 tgaccacgct cggtcatatt aatcgttcag caattaacgt gttgttattg cgccatgaac
     1081 gcagttctct gccgctcggc aagtttttgg tcaccgaagg cgttatcagc caggaaacgt
     1141 tggatcgcgt cctgacaatt caacgcgaat tacaagtttc gatgcaatca ctattactca
     1201 aagcaggttt aaacacagaa caggttgcgc aactggagtc cgaaaatgaa ggagaataac
     1261 cttaatcgcg tcatcggatg gtctggttta ctgctgacgt ctttattgag taccagcgca
     1321 ctcgcagaca atatcggcac cagcgcagaa gagctggggc tgagcgatta tcgccatttt
     1381 gttatttatc cccgtctcga taaggcgctg aaggcacaga aaaataacga cgaagcaacc
     1441 gccatccgcg aatttgaata tatacaccag caggtgccgg ataatattcc gctgacttta
     1501 taccttgcgg aagcctatcg ccattttggt catgatgacc gggcgcagct gttgcttgag
     1561 gatcaactga aacgtcaccc aggagatgcc cgacttgagc gcagtctggc ggctattccg
     1621 gttgaagtga aaagcgttac gactgttgaa gaactgcttg cccagcaaaa agcgtgcgac
     1681 gctgcgccga ccctgcgttg tcgcagtgaa gtcgggcaga atgccctgcg gctggcacaa
     1741 ttacctgtcg ccagagcgca actgaacgat gcgacgtttg ctgcatcgcc agaaggaaaa
     1801 acgctgcgaa ccgatctgct gcaacgggca atctacctga aacaatggtc ccaggcagat
     1861 acgctataca atgaagcacg ccagcagaac acattaagcg cggcagaacg ccgtcagtgg
     1921 tttgacgtgc ttcttgccgg gcagctggac gatcggatcc tggcactgca atcacagggg
     1981 atcttcaccg atcctcagtc atatattact tacgcgaccg cgctggctta tcgtggcgaa
     2041 aaagcacgcc tccagcatta tctcaatgaa aataagccgc tgtttaccac ggacgcacaa
     2101 gagaaaagtt ggctctatct gttatctaaa tacagcgcca accccgttca ggcattggcg
     2161 aattatacgg tgcagtttgc cgataaccgc cagtatgttg ttggcgcgac gctaccggtg
     2221 ctgttaaaag aaggtcagta cgacgcagcg caaaaactgc tcgccaccct ccccgccaat
     2281 gaaatgcttg aggagcgtta tgctgtcagc gtgccgaccc gtaacaaggc tgaatctctg
     2341 cgtctggcac gattgctgta tcagcaagaa ccggcaaatc ttacccgcct ggatcaactg
     2401 acctggcaac tgatgcagaa cgagcagtca cgcgaagctg ccgatttatt gctgcaacgc
     2461 tatcctttcc agggcgatgc gcgtgtcagc cagactttaa tggcgcgact ggcgtctctg
     2521 ctggaaagtc atccttacct ggcaacgccg gcgaaggtgg cgattttatc gaaaccctta
     2581 ccgctggcgg agcaacgtca gtggcaaagt cagttgccgg gtattgcaga taattgcccg
     2641 gcaatagttc gcttgctggg cgatatgtcg ccttcctacg atgctgccgc ctggaaccgt
     2701 ctggcaaagt gttatcggga cacgctaccc ggtgtggcgc tgtatgcatg gcttcaggcc
     2761 gaacaacgac aaccgaacgc ctggcaacat cgtgcggtag cctatcaggc gtatcaggtt
     2821 gaggactacg ccaccgcact ggcggcctgg cagaaaatca gtcttcacga catgagcaat
     2881 gaggatctgc ttgctgctgc caataccgcc caggcggcag gaaatggtgc agctcgcgat
     2941 cgctggctac aacaggcaga acaacgtgga ctgggaaaca atgccctcta ctggtggctg
     3001 catgcgcaac gttacattcc tggtcagccg gaactcgcac tgagcgatct cacgcgctca
     3061 atcaatattg cgccttctgc caacgcttac gttgcgcggg cgacaattta tcgccaacgt
     3121 cataatgtcc cggcggcggt gagtgatttg cgcgccgcgc tggaactgga accgaatact
     3181 agcaacatcc aggcagcgct tggttacgcc ttgtgggata gcggtgatat cgcacagtcg
     3241 cgggaaatgc tcgaacaggc gcataaaggg cttccggacg atccggcact gatccgacaa
     3301 ctggcctatg tgaaccagcg tctggatgac atgcctgcga cgcagcatta cgcccggctg
     3361 gtgattgatg acattgataa tcaggcgctg ataaccccac tgaccccaga gcaaaatcag
     3421 caacgcttca atttccgccg tttgcatgag gaggtcggtc gccgctggac gttcagtttc
     3481 gattcttcta tcggcttgcg ttccggggca atgagcaccg ctaacaataa tgtcggcggc
     3541 gcagcgccag ggaaaagcta tcgtagctac gggcaactgg aagccgagta ccgcatcgga
     3601 cgcaatatgc tgctggaagg cgacctgctc tcagtttata gccgcgtctt tgccgatacc
     3661 ggagaaaacg gggtgatgat gccggtgaaa aatccgatgt ccggcaccgg tctgcgctgg
     3721 aagccgctgc gcgatcagat ctttttcctc gccgtcgaac agcagttgcc gctgaacggg
     3781 caaaatggcg catccgatac catgctgcgc gccagcggct cattctttaa tggcggcaaa
     3841 tacagcgacg aatggcaccc gaacggttca ggctggtttg cccaaaacct gtacctcgat
     3901 gcggcgcaat atatccgcca ggatattcag gcgtggacgg cagattatcg cgtcagctgg
     3961 catcagaagg tagctaacgg acagactatt gagccttacg ctcacgttca ggacaacggc
     4021 tatcgtgata aaggcactca gggcgcgcag cttggcggag tcggggtccg ctggaatatc
     4081 tggaccggcg agacgcacta cgacgcctgg ccgcacaaag tcagtctcgg cgtcgagtat
     4141 caacatacct ttaaggcgat taatcaacgt aacggagagc gcaacaacgc gtttctcacc
     4201 attggagtgc actggtaaat gcgtaagttc attttcgtat tgctgacact gcttttggtc
     4261 agcccttttt cctttgcgat gaaaggtatt atctggcaac cacaaaaccg agatagtcag
     4321 gttaccgata cccagtggca ggggctgatg agtcagttac gtttgcaagg cttcgatacc
     4381 ctggttttgc aatggacccg ttacggcgat gcattcaccc agccagaaca gcgcgcgtta
     4441 ttgtttaagc gtgccgcagc tgcgcaacag gctggcctga agctgattgt cgggctgaac
     4501 gccgatccgg aattttttat gcaccagaaa cagtcgtccg cagcgctgga aagctatctt
     4561 aatcgcctgc tggctgccga tctccagcaa gccagattat ggagcgccgc gcctggcgta
     4621 acgccggatg gctggtacat cagcgcggaa attgacgacc tgaactggcg cagcgaagct
     4681 gcccgtcagc ctttgctaac atggttaaac aacgcgcagc ggctgattag cgatgtttca
     4741 gcaaaaccgg tttatatcag tagttttttc gccggaaaca tgtcgcccga tggctatcgc
     4801 caactgctgg aacaagttaa agcaaccggc gttaatgtct gggtacagga tggcagcggc
     4861 gtggataaac ttaccgctga acagcgtgaa cgttatctac aggccagcgc cgattgccaa
     4921 agtcccgccc ctgccagcgg cattgtttat gaactgtttg tcgccggcaa aggcaaaacc
     4981 tttacagcga aaccgaaacc ggatgcagag atcgcctcgc tgttagcgaa acgttcctct
     5041 tgcggtaaag acactctcta tttctctctg cgctatttgc ccgtcgcgca cggcattctc
     5101 gagtattaaa tctcctccag gtaagtcggg tacgacctgg cttacctctt tcgctctcgt
     5161 cagaattcca tcaagattaa ttgctaaaaa gcgtgctaaa gaaagaagat cgttatcggc
     5221 gaacgggccg atcactcatg caggaagcat agatcctttg agcggagttt ctgatatctg
     5281 aggagtgcga aatgcaattg agcagcagtg aaccttgcgt ggtgatcctg accgaaaaag
     5341 aggtagaggt aagcgtcaat aaccatgcta cgtttaccct tccgaaaaac tacctggccg
     5401 ccttcgcgtg caacaataac gtcattgaac tctcaacgtt aaatcacgta ttaatccccc
     5461 acatcaaccg taacatcatc aacgattatc tgttgttttt aaataagaac ttaacctgtg
     5521 taaagccctg gtcgcggctg gcaaccccgg ttatcgcttg tcatagccgt acaccggaag
     5581 tgttccggct agccgccaac cacagcaagc agcaacccag caaaccctgc gaagcggagt
     5641 tgacgcgcgc attacttttt accgtattgt ctaactttct tgagcaatcg cggtttatag
     5701 ccctactgat gtatatctta cgcagcagcg tccgcgacag cgtctgccgc attattcaaa
     5761 gcgatattca gcattactgg aatctgcgaa ttgtcgccag ttcgctatgt ttaagcccca
     5821 gcctactcaa aaagaaatta aaaaacgaaa ataccagcta tagccagatt gtcacagagt
     5881 gtcgtatgcg ttacgccgta cagatgttat tgatggataa caaaaatatc actcaggtgg
     5941 cgcaattatg tggctatagc agcacgtcgt actttatctc tgtttttaag gcgttttacg
     6001 gcctgacacc gttgaattat ctcgccaaac agcgacaaaa agtgatgtgg tgaagggcaa
     6061 agcggaaacg gataagacgg gcataaatga agaagaaatg gcgcgccctg caggattcga
     6121 acctgcggcc cacgacttag aagttcctag aacgacattt taagtcaaca aattaccgcg
     6181 ccatctctgc gctcacacgt cccactacct caaaacatgt aaagccttgc aagccattgt
     6241 gaggccttat gtgtctcagt tttgtccctc ttttttgtac taaaaaacat agcaattgag
     6301 gataaacctc atgctatttt cgcttatatg cctttaaagg catggcgatt aaatagataa
     6361 aaacaccaca aaagcataaa aaaccacaca gtaaaaccgc aacataaaac aataacagat
     6421 aattaaacca aaaacagata gcgcattgtg ataatcattc aatactaaac aaaatataaa
     6481 cagtggagca atatgtgatt gactcattaa gttagatata aaaaatacat attcaatcat
     6541 taaaacgatt gaatggagaa cttttatgcg ggcgaaactt ctgggaatag ttctgacaac
     6601 ccctattgcg atcagctctt ttgcttctac cgagactata tcgtttactc ctgacaacat
     6661 aaatgcggac attagtcttg gaactctgag cggaaaaaca aaagagcgcg tttatctagc
     6721 tgaagaagga ggccgaaaag tcagtcaact cgactggaaa ttcaataacg ctgcaattat
     6781 taaaggtgca attaattggg atttgatgcc ccagatatct attggggctg cgggctggac
     6841 tactctcggc agccgaggtg gcaatatggt cgatcaggac tggatggatt ccagtaaccc
     6901 cggaacctgg acagatgaaa gtaggcaccc tgatacacaa ctcaattatg ccaacgaatt
     6961 tgatctgaat atcaaaggct ggctcctcaa cgaacccaat taccgcctgg gactcatggc
     7021 cggatatcag gaaagccgtt atagctttac agccagaggt ggttcctata tctacagttc
     7081 tgaggaggga ttcagagatg atatcggctc cttcccgaat ggagaaagag caatcggcta
     7141 caaacaacgt tttaaaatgc cctacattgg cttgactgga agttatcgtt atgaagattt
     7201 tgagctaggt ggcacattta aatacagcgg ctgggtggaa gcatctgata acgatgagca
     7261 ctatgaccca ggaaaaagaa tcacttatcg cagtaaagtc aaagaccaaa attactattc
     7321 tgttgcagtc aatgcaggtt attacgtaac acctaacgca aaggtttatg ttgaaggcgc
     7381 atggaatcgg gttacgaata aaaaaggtaa tacttcactt tatgatcaca atgataacac
     7441 ttcagactac agcaaaaatg gtgcaggcat agaaaactat aacttcatca ctactgctgg
     7501 tcttaagtac accttttaac gaagttaacc agattttctc cccggtggta ttgtataacc
     7561 ccggggaatt tgtttacagt tacaagtagg caatatccac taattttgta agcatttaac
     7621 ctatgtcata acgtgtaatc tcttaccgca acacaagacc tcaatatatt caaatcttat
     7681 ctttattccg atattcagag acacaatgcg aaaaataatt actcatttca aagttgtttt
     7741 aacgttactt ctaccagtaa ccgtatctgc ccagcagata cagtggcaat cctgtatggc
     7801 cagtcaattc aaccactggt ttggtgagga aaaaccgtct cctgacttac tatgtggtta
     7861 tttgtctgtt ccattaaaat atacagacac aggcggagat gcttcttatg aaaaaaaatc
     7921 acaagtcaaa ctagcgttga caaaattgcc ggcaaaaagc aagcataaag gaagtatcct
     7981 gataataagt ggtggtcccg ggttaccagg cataaatcct tatattaact ttgactggcc
     8041 agtcacaaat cttcgtgagt catgggatat tattggattt gatcctcgag gcgtcggaca
     8101 gtccactccg acaataaact gccggcaatc agatacagag actcaggaaa acataaccga
     8161 aaagcaacaa gtattaaata aaattaatgc ctgtatccat aataccggag ccgaagtcat
     8221 tcgccatatc ggctctaacg aggctgtata cgatattgat cgtattaggc aagccttggg
     8281 ggataaacaa ctgacagccg tggcgtattc gtatggaact caaattgcag ccttatatgc
     8341 agaacgtttt ccctacaacg taagatctat cgttcttgat ggagtcgtcg atatcgatga
     8401 cctggaggac aacttcacat ggcaactcaa acaggcacag agttatcagg aaacgtttga
     8461 tcgctttgca tcctggtgtg cgcgtacaaa aagttgcccg ctttcttcag acagagataa
     8521 ggcaataact cagttccatg agctattatc aaaattacat cacaaacctt tattagacag
     8581 taagggagaa aatatatctt cagatgaact catatcatta acaacagacc ttctgctatg
     8641 gcgttcatca tggccaaccc ttgcaactgc catacgccag ttctctcagg ggattgtcag
     8701 taatgaaatt gaaactgcgc tcagtgctcc gatagcctca gaagagtcaa gcgatgcttc
     8761 gggggtaatc ctctgtgtag atcaggggga tgagcaatta acaccagaag agcgaaaatc
     8821 ccgaaaagac gctcttgcga atgccttccc ggctattaac tttgacaatg gacgttccga
     8881 ttcacctgat ttttgtgaat tatggccaat acatagcgac ctgaacaaaa ctcgcctgaa
     8941 aaatactgtt ctgccctctg gtttactgtt tgtagcacac aaatacgacc caacaacgcc
     9001 ctggattaat gcccgtaaga tggcagagaa attttccagc ccgttactaa caataaatgg
     9061 tgatgggcat acattagctc tcaccggagt taatttatgt gtagataaag cagttgtaca
     9121 tcacctgatc actccacaaa aaatagaaaa tatatactgc ccaggaaatt ctgaagcaga
     9181 aatacaataa tttcaaaata ttccgctatt tgctcccaat gcaaaacata tattgcattg
     9241 ggagcatatt catatttttg ttattattgt atgaaatcgt ctcatgtaca cggtcctttt
     9301 ttcgccgaaa actgatatta ctttaatcaa aagttgacgt gccagattcg aagtaaattt
     9361 ataaaatatc aaatcaatat acattttgca ccttcgaaat agtaatgact gttactgtaa
     9421 catgagttac aaacagcttc atacccagag ctactgatgt atcaactcga ttcaacaata
     9481 cccgatattt attccatgcc tccagcaacg atctttcttc ctccgttgcg atttccagat
     9541 ctacagcatc ctgcagtggc gcaatatact cactgaattc ctggatgtag aactgtgtag
     9601 tgacggtctt ccagccattc ggctcctgct gtatcgaagc ataccaggct atttcaatat
     9661 cgctatgctg cggcagcatt taaccccttg taattcatcg ccataattga tttaattcac
     9721 aaataaaact ataacatggt gaaattaatg aaaaaaaaca cagatgatgg ggctaaaatt
     9781 tacacaccac ttaccctaaa gctttatgac tggtgggttt tgggagtatc aaatcggctt
     9841 gcatggggat gtcctacaaa ggaacacctt cttccacact ttctggaaca tttaggtaac
     9901 aaccatctgg atattggtgt tggaactggg ttttacctta ctcacgtacc tgagagtagt
     9961 ctgatatctt taatggattt gaacgaagct agcctgaacg cggcatctac aagggctggg
    10021 gaatcaaaaa ttaaacataa aattaaccat gatgtttttg aaccttatcc cgcggcgtta
    10081 catggtcaat ttgattccat ttccatgttt taccttcttc actgcctgcc tggaaatata
    10141 tctacaaaaa gctgtgtaat acgcaatgcg gcgcaggcct taactgacga tggaactcta
    10201 tacggagcca caattcttgg cgatggagtt gtgcacaata gcttcggtca aaaactgatg
    10261 cgcatttaca atcagaaagg catcttttca aacacaaaag attccgaaga aggcttaaca
    10321 catatactct cagagcattt cgagaatgtt aaaaccaagg ttcaaggtac tgtagtaatg
    10381 ttttccgctt cagggaaaaa atagcatcca accgcagcac gttcttgcat aaggcgtgtc
    10441 tgcggttttt caactattca gatacatcac tcccatcaca ttcattcctc cgcatcatag
    10501 gcatataggc tatatcacct tgatattttt cttcttcaga taaaaactgt tatctatgta
    10561 tacttttaaa ccgaatccgt gtagagtctc tacctaagtt agttcgcagc taccctttca
    10621 gtttgcgcca ttaacctcct gacttttgcc gctatctgta agcattcctg tatacctgaa
    10681 actacagggt tcatctacga actttaagaa cacccaagat aaaaattgtc aactatatta
    10741 tatataacac catactaatt cgaggctata tgaacagcat actgataatc acttcactac
    10801 ttatcatatt cagcattttt ggtcatgctc taataaaatt agggattggc atatccaata
    10861 acccggacaa aaccgatgta taagtcaaca tatcctgaat cagacataca atattgcaat
    10921 gaaaaccaat aatattttaa ggaatatctt catgaaatca aaagacaccc taaagtggtt
    10981 ccctgcgcag cttcctgaag tcagaattat cctgggagat gctgtcgtgg aagtagcaaa
    11041 acagggaaga cctatcaata ccagaacatt gcttgattac attgaaggaa acataaagaa
    11101 aaagtcatgg ctggataaca aagaattatt acaaacagcg atatcagttc ttaaagacaa
    11161 ccaaaattta aatggtaaag tgtaataccc ccctgaaaaa taatttttca atgcattaaa
    11221 aaaatatcaa caactacgtt attccgcaac aacctacttt ttttaccatt tttccacttt
    11281 aacaacattt tgctccactt ttccacgacc aaacaacttg aaatctggtt aaaataacac
    11341 gcaacactat tcttcttcct tgagtccgcc cggaactcga aaaacaaacc gagttaaagc
    11401 tatttttcac aaaatcgatt ttgggtctca ccaaaattac ggggttgcat acgcattcgt
    11461 ttattttcga acgtgtacat acaaatatgc aca
//
LOCUS       DAMZET010000133        11358 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.133, whole genome shotgun sequence.
ACCESSION   DAMZET010000133 DAMZET010000000
VERSION     DAMZET010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.133"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..106)
                     /locus_tag="POB34_003571"
     CDS             complement(<1..106)
                     /locus_tag="POB34_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HDI5920452.1"
                     /translation="MSRVAAIIYALVICIIVCLSWAVNHYRDNAIAYKA"
     gene            complement(103..636)
                     /locus_tag="POB34_003572"
     CDS             complement(103..636)
                     /locus_tag="POB34_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDI5920453.1"
                     /translation="MNAKIRYGLSAAVLALIAVGAPAPDILDQFLDEKEGNHTTAYRD
                     GSGIWTICRGATVVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            742..1014
                     /locus_tag="POB34_003573"
     CDS             742..1014
                     /locus_tag="POB34_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920454.1"
                     /translation="MDNSTISLQELLDCISRLRDDVNALTVAFSYLAFSIPKEQMQPT
                     LASLQLESINPKWSQQQQNSFKWLAVLLEEKYAGEITISAESSVNQ"
     gene            complement(980..1324)
                     /locus_tag="POB34_003574"
     CDS             complement(980..1324)
                     /locus_tag="POB34_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HDI5920455.1"
                     /translation="MVQNYEMIVKGIRNFENKVTVTLALQDKERFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCGGDEQSPEKRPIILEAQSVWITYKGKLPG
                     RITGSLKTPPKW"
     gene            complement(1329..1544)
                     /locus_tag="POB34_003575"
     CDS             complement(1329..1544)
                     /locus_tag="POB34_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HDI5920456.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDRRKAARGE"
     gene            complement(1567..1758)
                     /locus_tag="POB34_003576"
     CDS             complement(1567..1758)
                     /locus_tag="POB34_003576"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920457.1"
                     /translation="MGDEQLLLLRLLAGMVLHEIRMLRGIFNENDNYCQLVVRIFSYM
                     TGKGDSGHQSFADGLFSFR"
     gene            complement(2638..3702)
                     /locus_tag="POB34_003577"
     CDS             complement(2638..3702)
                     /locus_tag="POB34_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDI5920458.1"
                     /translation="MHENTVKINSVDLINADCLHFIQSLPDNSIDLIVTDPPYFKVKP
                     NGWDNQWKGDEDYLKWLDHCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFNVLN
                     HIIWAKPSGRWNGCNKESLRAYFPATERVLFAEHYQGPYRGKSDGYAAKERELKQHIM
                     APLISYFRDARAELGITAKQIAEATGKKNMVSHWFGASQWQLPNEADYRKLQALFSRI
                     AAEKFQEQQLEQPHHQLVASYDSLNRKYSELLDEFKSLRRYFSVSVSVPYTDVWMHKP
                     VQFYPGKHPCEKPADMLRQIINASSRPGDLVADFFMGSGSTIKAAMALGRRALGVELE
                     TERFNQTIQEISMLSANTIL"
     gene            complement(3849..4052)
                     /locus_tag="POB34_003578"
     CDS             complement(3849..4052)
                     /locus_tag="POB34_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920459.1"
                     /translation="MLKQQDMTETAAAVLHFLPADKWVTPRMMTRTTGVSEARCQLIL
                     TQLVLAGLAKDNGGYGNKFRRCQ"
     gene            4372..5391
                     /locus_tag="POB34_003579"
     CDS             4372..5391
                     /locus_tag="POB34_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920460.1"
                     /translation="MDKFDEIHVDIESEQQAFDLLESYLDGYGLPDSLSFNGWPNLTI
                     RLTGEKFNRSLTPSVMKGFVEMQAQINKSYALAKYGVPDVRKLTKEELDALEIEVTVE
                     QGSSIVEINIDGFLTKLTQELVGKMNATEIMFTVLGAAVIWGGVTVFKRFLDNRKDIR
                     LAEIAKDGEKEHLRTMQIMSEQETKRLRVISEMIAEKPLLDNMDRMSYDAKTQMVKSF
                     VRSDNAQIDGVTIDSEMAKELVTNARRRSSEMRIDGIYRIEEVNNTDPESFKVKVRRV
                     DTDQRLTCVVQDVFLDESGNKEALQRAEWERKPVHLSINAKHVDGDIKSAVILYVRDV
                     EQKPE"
     gene            complement(5406..5786)
                     /locus_tag="POB34_003580"
     CDS             complement(5406..5786)
                     /locus_tag="POB34_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein Q"
                     /protein_id="HDI5920461.1"
                     /translation="MMRDIQMVLDRWGAWAASDSSGVDYSPIAAGFKGLLPYTSKTRQ
                     ACSDSDALIIEGCLARLRQKRPDEHSLLVAHYLYGISKRKLAKARKKDEKLIRIEIQM
                     AEGFIDGCLSMLEISLEMDPEIKD"
     gene            complement(5801..6790)
                     /locus_tag="POB34_003581"
     CDS             complement(5801..6790)
                     /locus_tag="POB34_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDI5920462.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPVVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            complement(6798..7595)
                     /locus_tag="POB34_003582"
     CDS             complement(6798..7595)
                     /locus_tag="POB34_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HDI5920463.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSLDKHKPAFWLRNE
                     QTERLISELQICNSVNIEPVNVIRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAHDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRTAINN
                     IKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            complement(7615..8004)
                     /locus_tag="POB34_003583"
     CDS             complement(7615..8004)
                     /locus_tag="POB34_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDI5920464.1"
                     /translation="MKLILPFPPSVNTYRRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKPAGAAA"
     gene            complement(8001..8327)
                     /locus_tag="POB34_003584"
     CDS             complement(8001..8327)
                     /locus_tag="POB34_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HDI5920465.1"
                     /translation="MTTLTQCQQQVLDMLISYQQERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKSVITIKRGVARGITLHTAVKDDDSEAVGIIRALLAGEENARLRAAHWLH
                     ERGLKV"
     gene            complement(8324..8977)
                     /locus_tag="POB34_003585"
     CDS             complement(8324..8977)
                     /locus_tag="POB34_003585"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HDI5920466.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            complement(8977..9471)
                     /locus_tag="POB34_003586"
     CDS             complement(8977..9471)
                     /locus_tag="POB34_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HDI5920467.1"
                     /translation="MIMSLLNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTEIPQEPEPQPVRESRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQQERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            complement(9468..10229)
                     /locus_tag="POB34_003587"
                     /pseudo
     CDS             complement(9468..10229)
                     /locus_tag="POB34_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104990.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            10227..10577
                     /locus_tag="POB34_003588"
                     /pseudo
     CDS             10227..10577
                     /locus_tag="POB34_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            10642..>11358
                     /locus_tag="POB34_003589"
     CDS             10642..>11358
                     /locus_tag="POB34_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HDI5920468.1"
                     /translation="MAVKISGVLKDGAGKPVQNCTIQLKAKRNSTTVVVNTVASENPD
                     EAGRYSMDVEYGQYRVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDAGTSAREAATHATDAAGSARAASTSAG
                     QAASSAQSASSSAGTASTKATEALKSAAAAESSKSAAATSAGAAKTSETNAAASQLSA
                     ATSASTATTKASEAATSARDA"
BASE COUNT         2843 a         3033 c         2683 g         2799 t
ORIGIN      
        1 gggctttgta ggcgattgcg ttatcacggt aatgattaac agcccatgac aggcagacga
       61 tgatgcagat aaccagagca taaataatcg cggcgactct gctcactgat ctatccccca
      121 acaggctaat gcgctttcct ggtcacgacg aataacctgt ccatagcagt tatttgaacg
      181 tatgcggcaa tcgcgcccac catcttttat ccaccagcga atcgcctcgc atgcaccttt
      241 acgatcaccg gcattcagcc gcttataaaa cgtcgacggg aaacacttac cggggccaat
      301 gttataggga caaaatgacg cgatacccgc tttctgtggt tcggtcagtg gcactttaat
      361 attgcgctcc acccatgcca gcgccttatc ccgttcaatg gcgttaacct ggtcgcattt
      421 ttccttcgac agtttcatac cgggaacaac gggttttcca tccaccaccg tggcaccccg
      481 acagatggtc cagatgccgg aaccatcgcg gtatgccgtt gtgtggttac cttctttttc
      541 atccagaaac tggtcgagaa tatcaggcgc aggcgcaccg acggcaatca gtgccagaac
      601 ggcagccgac aggccgtatc tgatttttgc gttcatggat atttatcagg atttatcggt
      661 ttctgaaccc tggatatgtt tatctgtccc ggcctgttga atcaggcaag gaatagttaa
      721 atacaataga gaggattgtt tatggacaat agcaccattt ctctacagga gttgctcgac
      781 tgcatttcca ggcttcggga tgatgtaaat gcccttactg tcgcattttc atatctggca
      841 ttctcaattc ccaaggaaca aatgcaacca acactggcat cgctccagct tgaatcaatc
      901 aatcccaaat ggtcccagca acaacaaaat tctttcaagt ggctggcggt attactggag
      961 gaaaaatatg ctggtgaaat taccatttcg gcggagtctt cagtgaacca gtaattcttc
     1021 ccggcagttt tcctttgtag gttatccaca cactctgcgc ctctaaaatt atggggcgct
     1081 tttccggcga ctgctcatcc cctccacata acccggcagc aacatccagg aagacctgtc
     1141 tgatgctcct tctggctgct gcctcataaa actccagcgc ggcaccttca acacggtcca
     1201 gcgagatgtc caggtcaaaa atttcaccgt caaagcgttc tttgtcctgt aatgctaaag
     1261 ttaccgtaac tttattctca aaattgcgga tccctttcac aatcatttca tagttttgga
     1321 ccattatatt actctcctct cgcagccttt cttctgtctt ctctgatttt gaagtacaga
     1381 ttcgtcagat aagtcagaaa gcccagaacc agacttccca gtacaccaat cgcagcccac
     1441 tgagacggac taacctgatc aagccattgt aaaaaccagt agccagcact gcctgcggag
     1501 gtgccgtagg caatgcctgt tgatattttt tccatctgat acatatcccg ccccaacaga
     1561 cctgtgctac cggaaagaaa aaaggccatc agcaaaactc tgatggcctg aatcaccttt
     1621 accagtcatg tatgaaaaaa tacgcacaac caattgacaa taattatcat tttcattaaa
     1681 aataccgcgt aacattctta tttcatgaag aaccataccc gccagcaacc tgagtagcaa
     1741 aagctgttca tcccccatat ttttgctggc gggttctttt ttctactgcc ctcctcccca
     1801 ggaaaagcat ttcattcttt tctcattaat cgttactgga gagaaggcgg gaaaaagcca
     1861 gctctttggc cctaaataac agcatggctt cagtccatac agaatctgat tttccagctc
     1921 acataataac cagatgatgc agttgcaaaa tctggcaaca tcggattatt atgcgcctgt
     1981 gaaaatttct tcacgtttta ttccttgccg cccgtgcctc ccagcgcggg cttttttatc
     2041 caaaaaatac ccctccggag aggggtatca tacaagggga tgaccatgat gcatggtgcc
     2101 gggtgcctcc cggtgagtcc ggcatcagca tacgaacccg cgattatccc agaactgact
     2161 gctgattcgc cccgccgctt agggggattc accatgcgtt tttttattta caaactcttc
     2221 gccagacaaa caaccattaa ctggctgaat tgtgaggtat ttcaattttt agaaaactga
     2281 ccaacctccg cttatacgcc gggcatggag cggatacaaa aaagccccgc aaaagcagat
     2341 cagaaggatt cgttgtaaaa aaaaagctgt cagattatcg cgctaatctg acagctacag
     2401 caggtatcca aaaacatggg tggaaacaat aagtgagtta agaagacacg gtgtcggatt
     2461 tttaccgatg agccgttggc gtggtcaacc gtgactcgct tgtatcaacg atgaattaat
     2521 gaagccaatc ctttgcaggc tatgtacaac ccaccacatc agccctgctg cattgcagga
     2581 tacactgtat ctgtaatcat tatacggcac agacatccca ccaataattg aattaattca
     2641 caaaattgta tttgctgata acatactgat ttcctggatg gtctgattaa accgctctgt
     2701 ctcaagctca acacctaacg cccgacgccc cagcgccatt gctgctttta ttgtggaacc
     2761 ggatcccata aagaaatcag caaccagatc gccgggtcga ctactggcat tgattatttg
     2821 cctgagcata tccgccggtt tctcacacgg atgtttaccc gggtagaact gaacgggttt
     2881 atgcatccag acatcggtat aaggcacgga gactgatacg gagaaatagc gccggagaga
     2941 tttaaactca tccagcaatt cagaatattt gcgattcagt gaatcataag atgccaccaa
     3001 ctggtggtgt ggttgttcca gttgttgttc ctgaaacttc tctgccgcta tacgggaaaa
     3061 cagtgcctgt aacttccggt agtcagcctc attcggcaac tgccactgac tggcaccaaa
     3121 ccagtgggaa accatatttt tcttacctgt ggcttcggca atttgttttg ccgttatacc
     3181 cagttcggca cgagcatccc tgaaatacga tatcagcggt gccattatgt gctgtttgag
     3241 ttccctttct tttgccgcat agccgtcact tttgccgcga tatggcccct ggtaatgttc
     3301 agcaaacaga acgcgctctg tggcaggaaa atatgcgcgc agactttctt tattacaccc
     3361 attccaacgt ccggacggct tcgcccagat gatatggtta agcacgttga aacgttcacg
     3421 catcatgatc tcaatatcag atgccaggcg atgcccacag aacaggtaaa ggcttccggc
     3481 tggtttcagt acccgccaga actgggccag acagtggtcc agccacttca ggtaatcttc
     3541 gtcccctttc cactgattgt cccagccgtt gggcttcact ttgaagtacg gcggatcggt
     3601 aacaatcaga tcaatggagt tatcaggcag ggactgaata aaatgcaggc aatcagcgtt
     3661 gattaaatca acactgttta tttttacagt attttcatgg atcagtaagc gtaactctgg
     3721 taggctcact ctgcttttgc gctaaagcag tgggccgtgg ttcgcttgtg acaagtaagc
     3781 atgagcgaat ggctggcagg tgctaccaac acccaccagc cgcccatttt cacagcagga
     3841 aaccgccatt actggcagcg tctgaattta ttcccgtacc cgccgttatc cttcgccaga
     3901 cccgccagaa ctaactgagt cagtattaac tggcaccggg cttcgcttac tccggtagtt
     3961 ctcgtcatca tgcgtggcgt tacccacttg tcagcaggta agaaatgaag gactgcggcg
     4021 gcggtttctg tcatatcttg ctgttttagc atgtcttttt cccttctggt taacatgaca
     4081 taccaataac tcttgtctaa aaagccagca agataaaaag tcagtattca cgaccaccag
     4141 cgtgtttact gtgctgcacc aagtttacag gtacaaaaaa accgctcagc ggcgggttta
     4201 agttgtgtgg cgaagtaacc actcttaaca cagtaatcta gaaaatgcgg accgcgttag
     4261 tgatttttat ctgtttttcc attatttttt tgccacacag cttaaaacga aaaataaagc
     4321 aggcgttgcc aaacctcacg gtgacagttg gcttagaatg aggacatatt tttggataag
     4381 tttgatgaga ttcatgtaga cattgaatct gagcagcaag ccttcgattt gcttgaaagc
     4441 tatcttgatg gttatggttt acctgacagt ttaagtttca acggatggcc aaaccttacc
     4501 atcagattaa ccggtgaaaa attcaacaga tcgctaacac cttcagttat gaagggattt
     4561 gttgaaatgc aagctcaaat caacaaatca tatgcccttg ccaagtacgg tgtccctgac
     4621 gtccgcaaac tgaccaaaga agaacttgat gccctagaaa tagaagttac ggtagaacag
     4681 ggttcttcaa tagtagaaat caacattgat ggctttttaa ccaaactcac acaggaactt
     4741 gttggtaaaa tgaacgcaac tgagatcatg tttactgttc tcggcgcagc agtcatctgg
     4801 ggcggagtaa cagtatttaa acggttcttg gacaaccgta aagatattcg ccttgcagaa
     4861 attgctaagg atggagagaa agaacatctc cggactatgc agatcatgag cgagcaagaa
     4921 actaaacgcc tgcgggttat ttcagaaatg attgcggaaa aaccacttct cgataacatg
     4981 gaccgtatgt cgtatgacgc taaaacccaa atggtcaaat cctttgtgcg ttcagataac
     5041 gctcaaattg atggcgttac tattgattct gaaatggcga aagagttggt tacgaatgct
     5101 cgtagacgtt catcagaaat gaggattgat ggtatttacc gaattgaaga agtcaataat
     5161 actgacccag agagtttcaa agttaaggtc cgacgcgtag ataccgacca acgtctaacc
     5221 tgcgtagttc aggatgtttt ccttgatgaa tccggaaaca aagaagcatt gcagcgagct
     5281 gagtgggaaa gaaagcctgt acatttgagc attaacgcaa aacacgtcga tggtgacatt
     5341 aagtcagcag taattctcta tgttcgagat gtggaacaaa agcccgagta atcgggcttc
     5401 aataatcaat ctttaatttc ggggtccatc tctagactaa tttccagcat cgataggcag
     5461 ccatcaataa acccctcagc catctgtatc tcaatgcgta ttagtttctc atccttttta
     5521 cgagccttgg cgagctttct tttagagata ccgtataggt aatgggcaac aagaagcgaa
     5581 tgttcgtccg gccttttttg ccttagacga gcaagacaac cttcaataat taatgcatca
     5641 ctatctgaac atgcctgacg tgttttgctt gtatagggaa gaagcccttt aaacccagca
     5701 gctataggag aatagtctac tcctgaacta tcactcgccg cccatgctcc ccaacgatcc
     5761 agaaccattt gaatatcacg catcaacttt ctccacaaaa tcaggccagc acgccaattg
     5821 ccagcgcacg atcgataaaa cgaaatatca gctccagctg ggagccatac ttctcttcaa
     5881 atgccacggt atccgcatgc agctcgtcgt gatgctttct gcacaaaggc aacacaaaga
     5941 ggtcatgcgc ttttgtaccc attcccccct gaccgtggcc tatcaggtgg tggggatcat
     6001 cagcaggctt tccacaacat gcacacggct gtgtcttaac ccagcgcgtg tacttttcat
     6061 taacccagcg gcgacgtttt gggcgtaaca taaaagactc cggcgactcc ggatccactt
     6121 tcagcgccag cacctttttc gccttatcct ggatgatgct ggtggcagga accgaaggca
     6181 caaggtcact ttcccgggtg acagacggca caacaggctt cggtaatctc agtgccttac
     6241 gggctgcact ttccggtaag gcatccgcca ggtcattacg aatcagccac cagcacagtt
     6301 ccggcattgt cacaacgtga ctgtcatcaa aaccgagatc ccgacgcaca acagacaaca
     6361 cccagcgggc acagttatcc gttgccattg attccagccg ttccgtgaac tgatcgcgca
     6421 gctggttatc gcagtgccag cacagacgga ttgcgcccgg cgcgtgtcgc attgttgtca
     6481 tgttctcgct gtgccagtcg gaatgaggcc actggcagcc tttttcacga agtaaccagc
     6541 tttcaagaca ttccacgcca ccagcacgac ggatcactgc ctcattgcgg aacacggccc
     6601 gaacgacagg atcatccgcc agcggttgtg atgccgccgg aacggcacca ctggcaaaag
     6661 atgaataacg ttccggctca ggctccagca ggacacgccc ctgcataaac aggggcatca
     6721 gctctgaacc aggtctgaac aatacgatcc ccatacgcgg ggcaatttca ggggtcagta
     6781 gtgctctcac agtcacctca atgaacggta tcgagcagct ttaacagctc agggaatcgg
     6841 gattcgaaga aatgcggctg cgtctcgcgc ggatttgcag gactggtgat gttcttgccg
     6901 aacatgcagc ctttcgctgt cagcgaccag aattttttga tgttgttaat cgcggtacgg
     6961 ctgtatcgtt cgcgctgctc gacgatcccc agcttcacca tctggtgata tgcctgatta
     7021 gctgtcaggc ggataccata ctgcttcagc agtgcactca gtgacagtgt cgggcgactt
     7081 gagccatcgt gtgcatcagc aggagcatca atggcatagc gcggtgccag attcggtaag
     7141 ccaacagcct cctggagttt ctgacaggca ccaagcactg aagagttaga caggtttaac
     7201 tcccggcgca taaagtccag caggatcacg ccagcctgca tcttgtcagc agcctgtccg
     7261 gataattttt ccggtgcgct ggttaccata tcgaaagtac ggatcacctt cagatggaat
     7321 gacgggctga tccacattgc ataggcatac accagttctt tgcagacata cgtcccctgg
     7381 ttatttccgc cacgaataac gttaactggc tctatattga ccgagttgca aatctgcaac
     7441 tcgcttatta aacgttcagt ttgctcattg cggagccaga atgcaggctt atgcttatcc
     7501 agagaaccgg cagccctgtg cagatcgttc aggctgtaac gaccataagc atcacgacga
     7561 acttcaatac catcaatgac catcagatta ttcatacttc gtttctcctc ttaatcaggc
     7621 ggctgcaccc gccggtttct cgtacttact gatagtgatc tcgaccttcc cttccgggat
     7681 aaccggtccc cactccacca gcattctttt cacctgactg tcgtcttccc acacacccgc
     7741 atgggtcagg gcgtcaaaca gcgccttgtt atagttgtcc agatcgcgga tccggttatc
     7801 cggaggaaac aacacgatct ccactgaagc aggtgccgac gttggtttcg gcagacgacg
     7861 taactgctca actattgctg cgcacgccgc gctctggaat ttgcgccccg ctgcgcttat
     7921 cagactctta ccagcaaacg cccctttgtt ggggtgtcgc ctgtacgtgt tcacgctggg
     7981 agggaaaggc aagatcagct tcatactttc aggcccctct catgtaacca gtgggctgca
     8041 cgcagcctgg cgttttcctc accggcaagc agtgcgcgga taatcccgac cgcctcgctg
     8101 tcgtcgtcct tcaccgcggt atgaagagtt atcccccggg ccacgccacg ctttatcgtg
     8161 atgacgcttt ttttctccag tgcgcgaaga tgctccaccg ctgcattcac cgaacggtat
     8221 cccagcatgg ttgccacctc ctgattggtt ggcggaaagc cacgttcttg ctggtaagaa
     8281 atcagcatat ccagcacctg ctgctggcat tgagttaacg tcgtcatgcc gccatctccc
     8341 tgaccagttt ttccgcctgc tggcgaacct gcgccagaaa ggcctcacca catgcctcaa
     8401 gttcatcgcg cccgatgtag ctgattgccg gtcccttcca ggtcttgtcg aaaacagcaa
     8461 tagcaccagc gaagaaagcg cctgtcggca cctgcttctc gtccttcggg ataaaccagg
     8521 ctggcagttc aaaaccaata cgcccgcgaa taaaagcaat atggtctgca tcttccggcc
     8581 accacacttc gctggtggca gctttgatca ggaaaacata gcgcccgcct ttatcacgca
     8641 tggcactggc atgcttcatg atgtaacgca tgccggtgat gtattgcccc tcatgctgac
     8701 tggcgcggct gtacggggga ttaccaaagg cagcaccttt aagctccgca agacgttctg
     8761 accagtcatg cgccagcgcg ttgtcttccg cagtgtaata cgcggcgcat ttggcgttat
     8821 caccatcagt aaacagatcc agaacaaacg ggccaaacag ggtgttaatt ccccagaaaa
     8881 tgttgtccgg cgtgcgccac tgatcgccca cttccttcag ttcatgggct ggtttgttcc
     8941 gcagctccac cagcgcctgg caatatttat tactcattaa gcccccacgt aattccctga
     9001 cagataccac tcttcacccg atgcagcgcg cttgctgctt ttccgtaaac accgctcacg
     9061 acgcgcaaga aaattgtttc gctcttgctg ggagtggctt tcacggaatg ccgccatcca
     9121 caccgttgca gcacgacggt ataagcccct ggactccagt tcttccgcct ggcgggtcag
     9181 gcacaaaatc acccggggat cgttagtgcc gacatagaaa ttgcgcacag gtctggattc
     9241 acgaactggt tgcggttccg gctcctgcgg tatctcagtc agccgcggga aatgtctgcg
     9301 tgtatcccct tcacaacggt gagccacacg cccactctga cgtaacttgc ttgctgactg
     9361 cagaacgcgc tgtcgtgagt aacctgcaaa agcatccgca atgtctccgg aagtacaccc
     9421 cggatgggct tcaatgaatt tctgaacgtc attcaaaaga ctcatgatca ccccctgaat
     9481 cctgccggga tctggctgta gtccacgttg tcgtaactgg ctttgaagta cgggtcttcg
     9541 cgtttttcgg tgtacgtgct gacggacggc gataagcgca gggaaagctc atcccatttt
     9601 tcccgcagct tcgacgggct gagcacgtta cggcaccaga acggatcgcg actgacgcgg
     9661 ctgtacatct cgcagatttg tttatgagta cgaccatcct gcacacacat caggcgaatt
     9721 tcgtttgccc aggctgtcca gttcggttct ttgggacgaa ccacctcgcc gtcacattcg
     9781 gcggcatgct cgtacagggc gatgattttt ttccagagcc actgtgcgca ggtcaaatca
     9841 tcctgcgtcc cccactggcg ctttttaggg ctgaatacaa ccgcatcagg atggcgagtt
     9901 aaaaaatcct gttcagccgt ctgcgtgtcc ggttgcgaag agtccggacg agaaggtttt
     9961 ttatctgacg gatcatgttt tgattttact gacggatccc cgccagattc tgacgggtga
    10021 aaacccgctt ttttgccaga tttcgacgca tcaaattttg acgggtcaga ttttgatgcg
    10081 tcagattttg acgggtcaga atctgacagt tgagaaaatg ccgctgcctg aagcttcgca
    10141 acgttaagct gataaacatt cgacgcattg cggttaccct ggcgacgcgc cttacgcgtt
    10201 aaccagcctt ctgcttccag ccgtgcgata cccgctggca tgaagattcc gtgcgtaacc
    10261 gctggttcag cgtgatggcg gggccgtctg tgcgcgtgaa tgaatggttc agcgcgtatg
    10321 cgatggcggg tgtggcttac agccgtattt cgactttctc cggggattat ctccgcgtaa
    10381 ctgacaacaa ggggaaaacg cacgacgtgc tgaccggaag tgatgacggt cgccgcagca
    10441 acacgtctct ggcgtggggg gctggcgtgc agtttaaccc gaccgaatcc gtggccattg
    10501 atattgctta tgaaggctcc ggcagtggcg actggcgcac tgacggtttc atcgtgggtg
    10561 tcggttataa attctgatta gccaggtaac acagtgttat gacagcccgc cggttcaggc
    10621 gggctttttt gtggggtgaa tatggcagta aagatttcag gtgtactgaa agacggtgca
    10681 ggtaaaccgg tacagaactg cacaatccag ctgaaagcaa aacgtaacag caccacggtg
    10741 gtggtgaaca cggtggcatc tgaaaatccg gatgaagccg ggcgttacag catggacgtt
    10801 gagtacggtc agtaccgcgt tattctgttg gtggaaggat tcccgccgtc acatgccggg
    10861 accatcaccg tgtatgaaga ttctcaaccc ggtacgctga atgattttct cggtgccatg
    10921 acggaggatg atgcccgtcc ggaggcactg cgccgttttg aactgatggt ggaagaggtg
    10981 gcgcgtaacg cgtccgcagt ggcacagaac acggcagccg caaagaagtc agccagcgat
    11041 gccggcacat ctgcccgtga ggcggcaacc catgcaaccg atgctgcagg ctcagcacgt
    11101 gccgccagca cgtcagccgg acaggctgca tcatctgctc agtcagcatc ttccagcgca
    11161 ggaacggcat caacaaaggc cactgaagca ttaaaaagtg ctgccgctgc agagtcctca
    11221 aaaagcgcgg cagccaccag tgccggtgcg gcgaaaacgt cagaaacgaa tgccgcagca
    11281 tcacaactat cagcagccac ttctgcatcc accgcgacca cgaaggcgtc agaagctgcc
    11341 acctcagccc gggatgcg
//
LOCUS       DAMZET010000134        11350 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.134, whole genome shotgun sequence.
ACCESSION   DAMZET010000134 DAMZET010000000
VERSION     DAMZET010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.134"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(124..1035)
                     /gene="rdgC"
                     /locus_tag="POB34_003590"
     CDS             complement(124..1035)
                     /gene="rdgC"
                     /locus_tag="POB34_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDI5920469.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            1160..2068
                     /gene="mak"
                     /locus_tag="POB34_003591"
     CDS             1160..2068
                     /gene="mak"
                     /locus_tag="POB34_003591"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HDI5920470.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(2211..3395)
                     /gene="araJ"
                     /locus_tag="POB34_003592"
     CDS             complement(2211..3395)
                     /gene="araJ"
                     /locus_tag="POB34_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HDI5920471.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYMMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(3521..6664)
                     /gene="sbcC"
                     /locus_tag="POB34_003593"
     CDS             complement(3521..6664)
                     /gene="sbcC"
                     /locus_tag="POB34_003593"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HDI5920472.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRQDELQQEASRRQQALQQVLAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNTLAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVKVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(6661..7863)
                     /gene="sbcD"
                     /locus_tag="POB34_003594"
     CDS             complement(6661..7863)
                     /gene="sbcD"
                     /locus_tag="POB34_003594"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HDI5920473.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            8053..8742
                     /gene="phoB"
                     /locus_tag="POB34_003595"
     CDS             8053..8742
                     /gene="phoB"
                     /locus_tag="POB34_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HDI5920474.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            8800..10095
                     /gene="phoR"
                     /locus_tag="POB34_003596"
     CDS             8800..10095
                     /gene="phoR"
                     /locus_tag="POB34_003596"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HDI5920475.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            10502..>11350
                     /gene="brnQ"
                     /locus_tag="POB34_003597"
     CDS             10502..>11350
                     /gene="brnQ"
                     /locus_tag="POB34_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDI5920476.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLL"
BASE COUNT         2518 a         2937 c         3038 g         2857 t
ORIGIN      
        1 gcataatatt gctgcgctaa aaattattgc tcgccgtagc gaataattat atgcctggtg
       61 tggcttcgta cgccggataa gacgcggcag gcgtcgcatc cggcattaaa ggaaaatcag
      121 caattaacgt tgtgcttcgc cacctaatcc ttcaatcagg ttttgaatta acgctgccag
      181 ttcaccagtc atcaggatga aatcggcatc aaaacgctgg gcgaaatctt cacggtcgat
      241 atcttcgttt tgatcgcgca gctcgtcgca gaacttcaga cgcttgagcg aaccatcgtc
      301 gcacatcaca aactgaatgc gctgctgcca gtcgagcgcc agtttagtca ccacttttcc
      361 ggcttcaatg tgattggtga tctcttcgct ggtcagatct tgtttcttcg cgcggatcac
      421 gccgccatct tccagcaacg atttcagctc ggcttcatca agcagctgga agccctgtgc
      481 cgcactaccg gagcgaaccc attcggtcag cgtcagttca atcgggtttt ccatgctcaa
      541 tggtacaacc ggtaacgacc ccaggctttt acgcagtaat gccagcgtat cttccgcttt
      601 tttggcactg gcgcagtcca ccataatcaa accattaacc gtgtcgatcc acatcattgt
      661 ctggctaaaa cggctgaaag cacgcggcaa cagagagtgc agcacttcgt ctttcagcga
      721 atctttttcg gttttcttca gcttacgcgc ctgttccgct tccagtttgg cgattttcgc
      781 ttccagcgcc tgtttaatca ccggggacgg gaggattttt tcttctttgc gcgcgcagat
      841 aacaatttga ccattggcaa cgtgcgttaa cgcatcgctg tgcgatccca tcggaggaac
      901 ccagcccatc ttcgccatgt cctggctgcc gcatggggta aatgccatcg aggctagctg
      961 tttttccatc tcttctgcac gcagcgaaat ctcgcggcta agacggtaaa ccattaaatt
     1021 tttgaaccac agcatgataa tttccacggc cttgtcgtta aatttagcgg gcatgataac
     1081 gaattgtcgg cggccttgca ttgccaatcc ggttgtccgt ctctacgcta ttgatattga
     1141 aaaaaataag gagagtaccg tgcgtatagg tatcgattta ggcggcacca aaactgaagt
     1201 gattgcactg ggcgatgcag gggagcagtt gtaccgccat cgtctgccca cgccgcgtga
     1261 tgattaccgg cagactattg aaacgatcgc cacgttggtt gatatggcgg agcaggcgac
     1321 agggcagcgc ggaacggtag gtatgggcat tcctggctca atttcgcctt acaccggtgt
     1381 ggttaagaat gccaattcaa cctggctcaa cggtcagcca ttcgataaag acttaagcgc
     1441 gaggttgcag cgggaagtgc ggctggcaaa tgacgccaac tgtctggcgg tttcagaagc
     1501 agtagatggc gcggcagcgg gagcgcagac agtatttgcc gtgattatcg gcacgggatg
     1561 cggcgcgggc gtggcattca atgggcgggc gcatatcggc ggcaatggca cggcaggtga
     1621 gtggggacac aatccgctac cgtggatgga cgaagacgaa ctgcgttatc gcgaggaagt
     1681 cccttgttat tgcggtaaac aaggttgtat tgaaaccttt atttcgggca cgggattcgc
     1741 gacggattat cgtcgtttga gcggacatgc gctgaaaggc agtgaaatta tccgcctggt
     1801 tgaagaaagc gatccggtag cggaactggc attgcgtcgc tacgagctgc ggctggcaaa
     1861 atcgctggca catgtcgtga atattctcga tccggatgtg attgtcctgg ggggcgggat
     1921 gagcaatgta gaccgtttat atcaaacggt tgggcagttg attaaacaat ttgtcttcgg
     1981 cggcgaatgt gaaacgccgg tgcgtaaggc gaagcacggt gattccagcg gcgtacgcgg
     2041 cgctgcgtgg ttatggccac aagagtaaaa aacgtaggca attggcgcat catgcttgat
     2101 gcgacgcttg ccgcgtctta tcaggcctac aaaatgtgcc ggaaccgcgt agggcggata
     2161 aggcgttcac gccgcatccg gcaataagca acgcgcaacg tgactgcaat ctaccccagt
     2221 ggtttcgcca gcaccggact atccgccgct tgctggcgct tatagcgacc atacagcagc
     2281 aatgacgaca tcgcagcaaa cgaaagcagg gcggcaggca gcgccacgta attatatgcc
     2341 agccccagcg tcagcatcat accaccgcaa tatgcgccga cggcgctacc aaggttaaac
     2401 gctatttgcc cacctgcggc acctaataac tctccgcctt tggcattttg tagtaataat
     2461 atttgcagag gtgctgaaag ggcaaataac cccgcgcaac aaataaaagc aaaaataagc
     2521 gacgttgttt tcatgccgcc gcagaaaaag agcatcagca gtgccagtac aattataaag
     2581 tcagtcactg ctgcaatgcg cagtggtgaa taacgtcctg aaatcctgcc acttagcata
     2641 tttcccaaca ccatccctag cccaactaac atcataataa aggtcatcgc cgtttccgaa
     2701 aaaccggaaa taaacatcat gtatggcttt acgtagctga accaggcaaa cacacctgcg
     2761 ttgccaaaca tcgtggcggc gaaaattaac cacggggccg ggctgcgtaa aaagtgaaat
     2821 tgttcgcgca gctttccttt cgcctcgtcg cgaatatccg gcacccaaaa atagaccgat
     2881 gccatcaccg caatattaaa aacagcgatc aataaaaagg tgtaacgcca gctaaattcc
     2941 tgacttaaat acgttcccag cggaatgccc agcaaattgg cgactgtcat cccggaaacc
     3001 atccccgcca cggcggcggt gacttttccg ggtttgataa tttttgataa cacgatcgcg
     3061 ccgacgccaa aaaatgcgcc atgcggaaag ccggaaacca ggcgaccaat ggcaagcatc
     3121 atgtaagacg aagagagcgt gaacatggcg ttgccaatga cgcacaacgc caccagaaac
     3181 aacaagatat gtttgagtga gtagcggctg gaaaagagtg cgatgattgg cgcaccgacc
     3241 accactccca gcgcataata cgagatcata tgcccggcag caggaatcga aattcctacg
     3301 ttatgcgcca gctccgtgag cacgcccata atgccaaatt cggccatccc caaaccaaac
     3361 gtgcccagag ccaaagataa aatgactttt ttcataccac catcacaact accagaacgg
     3421 ctaccaatca ccgtagccaa taaagtgata gcttgcagga taacgaccag caaggccaag
     3481 agataattcg cccctctgta ttcattatcc tgctgaatag ttatttcact gcaaacgtac
     3541 tttccagttt gctgtagccc aggccgttga tctttttcac tttgatctgc accggaatac
     3601 gctctttcat cgcttctaca tggctaatca caccgatggt tttgccactg gcgttcaggg
     3661 catccagcgc atcaagggcg gtatccagcg tttcgctatc cagcgtgcca aaaccttcat
     3721 caaggaacag cgagtcaata cgtgttttat ggctgaccag atccgaaagc gccagcgcca
     3781 gcgccagact aacgaggaaa ctttcgccgc cggaaagggt acgggtatcg cgtaccgcat
     3841 ctgcctgcca ggtatcaaca accttgactt ccagcgcctc gctggctttg cgctgtaaca
     3901 gatagcgccc gtgcagccgg gtaagttgct gattagcgag atggactaaa ttatccagcg
     3961 tcagcccctg ggcaaacttg cggaatttat cgccctcttt ggaacctatt agcgaattca
     4021 gatatcccca gtcctcaacc tgctgcgtca tttgagcaat ttgctgcatt aaggtttgtt
     4081 gttgctgacg gttatctgca tcctgcttca gctgctggcg aatctcgcct tgactcgtgg
     4141 tgttttcacg caacttttgg tgagtttgcg ctaactcttg ctgaatctgc tccaccgtca
     4201 cagtgagagc caacccgtca ggtcggtgtt gttgatgctg tgccagcgtt tctgctgtct
     4261 gagtgaccag cgtttgcgcc tgacggcgct ggttttccag attctgcttg agctgttcca
     4321 gctgcgttag tgtttgttca tccattagcg ccgcaaggaa cgcctgctga tcgtcaaaga
     4381 cgctggcctg tagcgcggtg tcaaactgcg cctgggcttt ttgcagactt tgcgccgcca
     4441 gaacgtcctg ttgctgtaac gtctgctgct ggctgtgtaa tgcgagacat tgttcatgaa
     4501 cctgccgcca gttatccagc gccacagttt cttcgctgtg cgggagatca tcactttgcg
     4561 gcaacgtttc cagaatcggc gtcagctgct gaatacggtt ttgcagcgcg gttaattcgt
     4621 tctggcgttg ctgccagctc tgcgcttctt gctgacgtgt cgccaaccag ctctcttctt
     4681 catcttcctg tggcaatgtc agtgcataac ccgccaatgc cgttaagagc tgctgctggc
     4741 gttgttcaat ttgctgttga tactggataa tttgctgatt atgcgcggca atctgccctt
     4801 gtaattcatg tcgttggctg agtaaccgca gctggcgttc gtgctcatct tgtgcatcca
     4861 gccacggttg aatatcgtcc tgtggctgca aggtgatatt gaggctggcc gtgacggctt
     4921 gccattgttg agtaagtgct tgctcatctt gtcggaggct ttgcgcttcg ttttcatcgc
     4981 gctgaagctg cttcgttaat gcatccagtt gcccgcgcag cgccgcacct tcttcgccga
     5041 gctttttaac ttcgttttcc agcgccagta atcgagactg attaacacca ggctccagcg
     5101 cctgatacgc ctcgaccgcc gggtggctgg tggaaccaca aagtggacaa ggctgacccg
     5161 cctgtaactg tgcacgttga gcttccagcg ttttgatgcg cgcttcctgc tcgcaaatgg
     5221 ttttcacatc ggcaagttgc tgcgtctttt ctttataacg ctggcgcatt tcgttaagtg
     5281 cggcgttacg ttgcgtctgt tcttgcgtga cattctggat agcgacctgt aactgcgcca
     5341 gacgtttttg ttggggaaca atctgcccat gcagcgcgac caggcgctga cgcagtgggc
     5401 gttgctcggc atgttgcgcc agggcgctag caacttcatc ggcggttaac gtcaacgtga
     5461 tcgccgcaag cgtattaagt ttttgctcag catgggttaa ctgttgctgc cattgccgca
     5521 gatgctcgcg atcgctggtt tgttgggaga actgcgcacg ccaacccgcc agttcgttgt
     5581 tccactgacg gaagcggtcg tgttcctgta accaggtatt caggctttgt tgctgctgct
     5641 gtaattctgc tgactgcttc gccgcgtggt ggcgaatgct cgcgcgaagc gccattgtgc
     5701 tctgtaagcg agtatttact tcttcaatct gctggcgaat atgcgccagc gccgcgctgt
     5761 gttctgcgat gcgttcccag tgtggacgaa gatttcgtgc cggttgtgcc agactaagcg
     5821 ccgccagttg aggttgcgct ttttcttctt cggctaacac ctgttgcaag gcctgctgac
     5881 ggcggctggc ttcttgctgc aattcgtcct gacgcgttaa ccagtttagc gattgttgtt
     5941 cttgctgctg cgcggtaagt aactgttttt cttcgtcagt aagtacctgc aaactcgctg
     6001 tcagcgattg cacttgttcc ggcgtgagca acgcgacgcc gctggcctgc gcttgcagct
     6061 tctccagctc tgtgcgggcc gatttgtgct gctcaaaaac catcgccgag atttgcccgt
     6121 agatttcagt gccggttaac tcctcgagca attccgcgcg ttctttgggt ttggcattca
     6181 ggaaggcagc aaattgcccc tgcgaaagca gcatcgaacg ggtgaagcgc ccgtaatcca
     6241 gcccggttaa cgtcgctgtc agttccagct tatctttcac tttgtcggcg agaattttgc
     6301 cgtcggcgca gcgcgccagc tctacgcgtg gcacctgcaa attaccgtcg ggttggttac
     6361 gcgcccgatt ctggctccag aatgcacggt acgcttcacc tttcacttca aactccacct
     6421 ccgccagaca ttcagcggta tcgcgggtca tgagatcatt ttgcgattgt gaaacgttag
     6481 agagacgcgg agtttcatga tacagcgcca gacaaatggc gtccagcagg gtggttttcc
     6541 ccgcaccggt tgggccggta atggcaaaca gcccgttgct ggcgaacggc tcgcgggtga
     6601 aatcaatctt ccattcgcct tttaatgagt tcaggttttt caggcgcagg ctgagaattt
     6661 tcatgcttcg tgttctccgg cgagggtatg caacgtcgtg gtgaaaagat gctgcagacg
     6721 ttgctgctgc gattcatcca gttcttccag tgccagacgg cgattgaaca cctcttcgac
     6781 gctgagttcg ctgagggttt cacgctgttg gctggctaac acacgctcgc gctgttcacg
     6841 actccggcgt accagcaata cttcaacagg caatgattcg gttaatgcct ggattttgcg
     6901 ctgaatatca tgcagatact catcagtagt gatttcgata tccagccaga caggcggctc
     6961 ctgtgataca tcgcgccact gttccagctg tgcggtaatc gacgccagat cgcctttcag
     7021 cactgccatg ggttgcgtta ccggtacgtt caggttttcc acgctctcta atttgccgtt
     7081 tgaaaatgtc accagatgga catatttact cttaccgcat tcatcaaaac tcagtggaat
     7141 gggggagccg caatagcgaa catgttccat gccgccaata atctgtgcgc ggtgaatatg
     7201 cccgagcgcg atgtagtcgg ctggtggaaa gttttgtgcc ggaaacgcgt ccagcgtgcc
     7261 aatataaatg tcacgcacgg cgtcactttt actggccccc acggtcgtta aatgtcccgt
     7321 ggcgatgatg ggcagaggct gatcgccgcg cagtttgcag gcatcggcat agtgttgttg
     7381 gtaataatcg gtaatcgctg ccagtaaatg ctgctgtttt tcaataccgt taagccccgc
     7441 ctggctggta ataatgtcac gtggacgtaa aaacggaatg gggcacagca ctgcgcctgg
     7501 cgtcccgtcg cgacgaggca agatttgcgg cgcatgtccg gcgctggcga ccacggtagt
     7561 attgaggaac gccatgatat cgcgcgattc attcagcgtg gcgaccgagt catggtttcc
     7621 tgccagtacc accagatgac agccagtttg ctgtaaattg acaacaaaac ggttgtataa
     7681 cgtgcgggcg taactgggcg gcgagccggt atcgaaaaca tcaccagcaa caataatcgc
     7741 atccacctga tgggtttgtg ctgtctccag cagccagtca agaaaagcct gatgttcagc
     7801 ttcgcggctt ttactgtaga agttctggcc gagatgccag tctgaggtgt gaaggatgcg
     7861 cataacggtt ccctggcgaa aaagcatggg cgcgattata cccaaacaga tgtgccattt
     7921 gcttttttct gcgccacgga aatcaataac ctgaagatat gtgcgacgag cttttcataa
     7981 atctgtcata aatctgacgc ataatgacgt cgcattaatg atcgcaacct atttattaca
     8041 acagggcaaa tcatggcgag acgtattctg gtcgtagaag atgaagctcc aattcgcgaa
     8101 atggtctgct tcgtgctcga acaaaatggc tttcagccgg tcgaagcgga agattatgac
     8161 agtgctgtga atcaactgaa tgaaccctgg ccggatttaa ttctccttga ctggatgtta
     8221 cctggcggct ccggtatcca gttcatcaaa cacctcaagc gcgagtcgat gacccgggat
     8281 attccagtgg tgatgttgac cgccagaggg gaagaagaag atcgcgtgcg cggccttgaa
     8341 accggcgcgg atgactatat caccaagccg ttttcgccga aggagctggt ggcgcgaatc
     8401 aaagcggtaa tgcgccgtat ttcgccaatg gcggtggaag aggtgattga gatgcaggga
     8461 ttaagtctcg acccgacatc tcaccgagtg atggcgggcg aagagccgct ggagatgggg
     8521 ccgacagaat ttaaactgct gcactttttt atgacgcatc ctgagcgcgt gtacagccgc
     8581 gagcagctgt taaaccacgt ctggggaact aacgtgtatg tggaagaccg cacggtcgat
     8641 gtccacattc gtcgcctgcg taaagcactg gagcccggcg ggcatgaccg catggtgcag
     8701 accgtgcgcg gtacaggata tcgtttttca acccgctttt aacgccttgc tcatcggacg
     8761 cagagcaggg cttatgattt cttaactgga gtatcttacg tgctggaacg gctgtcgtgg
     8821 aaaaggctgg tgctggagct gctactttgc tgcctcccgg ctttcatcct gggtgcattt
     8881 tttggttacc tgccctggtt tttgctggca tcggtaacag gactgcttat ctggcatttc
     8941 tggaatttat tgcgcctttc atggtggctg tgggtggatc gcagtatgac cccgccaccg
     9001 gggcgtggta gctgggaacc gctactatac ggcttacacc agatgcagct gcgaaataaa
     9061 aaacgccgcc gtgaactggg caatctgatt aaacgctttc gtagcggcgc ggagtcgctg
     9121 cccgacgcgg tggtgctgac cacggaagag ggcggtattt tctggtgtaa cggtctggcg
     9181 caacaaattc ttggtttgcg ctggccggaa gataacgggc agaacatcct taacctactg
     9241 cgttacccgg agtttacgca atatttgaaa acgcgtgatt tttctcgccc gctcaatctg
     9301 gtgctcaaca ccgggcggca tctggaaatt cgcgtcatgc cttataccca caaacagttg
     9361 ctgatggtgg cgcgtgatgt cacgcaaatg catcaactgg aaggggcgcg gcgtaacttt
     9421 tttgccaacg tgagccatga gttacgtacg ccattgaccg tgttacaggg ttacctggag
     9481 atgatggatg agcagccgct ggaaggcgcg gtacgcgaaa aagcgttgca caccatgcgc
     9541 gagcagaccc agcggatgga agggctggtg aagcagttgc taacgctgtc gaaaattgaa
     9601 gccgcaccga cgcaattgct caatgaaaag gttgatgtgc cgatgatgct gcgcgttgtt
     9661 gagcgcgagg ctcagactct gagtcagaaa aaacagacat ttacctttga gatagataac
     9721 ggcctcaagg tgtctggcaa cgaagatcag ctacgcagtg cgatttcgaa cctggtctat
     9781 aacgccgtga atcatacgcc ggaaggcacg catatcaccg tacgctggca gcgagtgccg
     9841 cacggtgccg aatttagcgt tgaagataac ggaccgggca ttgcaccgga gcatattccg
     9901 cgcctgaccg agcgttttta tcgcgttgat aaagcgcgtt cccggcaaac cggcggtagc
     9961 ggattagggt tagcgatcgt gaaacatgcg gtgaatcatc acgaaagtcg cctgaatatt
    10021 gagagtacag taggaaaagg aacacgtttc agttttgtta tcccggaacg tttaattgcc
    10081 aaaaacagcg attaatccgc ctttgtcatc ttttattgcc ataagccagc cgatgctggc
    10141 ttattttctt tgcagtcaaa atacgggcgt tagattttac aacggttggt gattttttgt
    10201 tcgcatgatt agccatgtct ttttcacgga aatagtgttt tatactggtt ggtgatttct
    10261 tatcgctata tacctctggt ttttagatcc ctccttgctt taaaacgtta taagcgttta
    10321 aattgcgctt caggtgctgt catactgact gcattaacgc ggtaaatcga aaaactattc
    10381 ttcgccgcgc ctggttggga gtatttcccg ctaaaattgt ttaaatatac cgctgtatca
    10441 tccccaggga ttggcacaaa aatttaacgt tacaacacca catccacagg cagtatgatt
    10501 tatgacccat caattaagat cgcgcgatat catcgctctg ggctttatga catttgcgtt
    10561 gttcgtcggc gcaggtaaca ttattttccc tccaatggtc ggcttacagg caggcgaaca
    10621 cgtctggact gcggcattcg gcttcctcat tactgccgtt ggcctgccgg tattaacggt
    10681 agtggcgctg gcaaaagttg gcggcggtgt tgacagcctc agcacgccaa tcggtaaagt
    10741 cgctggcgta ctgctggcaa cggtttgtta cctggcggtg gggccgcttt tcgctacgcc
    10801 gcgtacagct accgtttctt ttgaagtggg gattgcgccg ctgacgggtg attccgcact
    10861 gccgctgttt atctacagcc tggtctattt cgctatcgtt attctggttt cgctctatcc
    10921 gggcaagctg ctggataccg tgggcaactt ccttgcgccg ctgaaaatta tcgcgctggt
    10981 catcctgtct gttgccgcga ttgtctggcc ggcgggttct atcagcacgg cgactgaggc
    11041 ttatcaaaac gctgcgtttt ctaacggctt cgttaacggc tatctgacca tggatacgct
    11101 gggcgcaatg gtgtttggta tcgttattgt taacgcggcg cgttctcgtg gcgttaccga
    11161 agcgcgtctg ctgacccgtt ataccgtctg ggctggcctg atggcgggtg ttggtctgac
    11221 tctgctgtac ctggcgctgt tccgtctggg ttcagacagc gcgtcgctgg tcgatcagtc
    11281 tgcaaacggc gctgctattc tgcatgctta cgttcagcac acctttggcg gcggcggtag
    11341 cttcctgctg
//
LOCUS       DAMZET010000135        11308 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.135, whole genome shotgun sequence.
ACCESSION   DAMZET010000135 DAMZET010000000
VERSION     DAMZET010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.135"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(164..677)
                     /locus_tag="POB34_003598"
                     /pseudo
     CDS             complement(164..677)
                     /locus_tag="POB34_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
     gene            complement(738..842)
                     /locus_tag="POB34_003599"
                     /pseudo
     CDS             complement(738..842)
                     /locus_tag="POB34_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(844..1167)
                     /locus_tag="POB34_003600"
                     /pseudo
     CDS             complement(844..1167)
                     /locus_tag="POB34_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1913..2890
                     /locus_tag="POB34_003601"
     CDS             1913..2890
                     /locus_tag="POB34_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDI5920477.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLQPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(3175..3915)
                     /locus_tag="POB34_003602"
     CDS             complement(3175..3915)
                     /locus_tag="POB34_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI5920478.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLNGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALVGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(4036..4224)
                     /locus_tag="POB34_003603"
     CDS             complement(4036..4224)
                     /locus_tag="POB34_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HDI5920479.1"
                     /translation="MESEIFSCLLRASAGFFLRFCLSWHYPNSPKNGKPVLSPHHDWL
                     KTGSLRQNAPLRIWFYPQ"
     gene            complement(4598..5500)
                     /locus_tag="POB34_003604"
     CDS             complement(4598..5500)
                     /locus_tag="POB34_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antimicrobial resistance protein Mig-14"
                     /protein_id="HDI5920480.1"
                     /translation="MSIKNIKRIITAWKPSTFETYKKTFEKYGGSVNMHPDVVSYFMI
                     HHDWKFDFFHYEKDGDIKGSYFLCNGKQIGIMARRSYPLSSDEVLIPFSPHARCFFPD
                     KTNKLSIINKQNIINATWKIARKKQNCIIKESFSPKFEKTRRNEIQRFIRNGGEIKCI
                     SQLSDKEISSSYISLFHSRFGGTLPCYEYDNLLMFISHLRELMFGHVLFWDNKPCAID
                     IVLKSESSCNVYYDVPNGAVLNDENCMKLSPGSVLMWLNIDKARRYCQDNNKKMIYSI
                     GAFRPEWKYKLLWSVPCKVGKCLC"
     gene            complement(5569..6678)
                     /locus_tag="POB34_003605"
     CDS             complement(5569..6678)
                     /locus_tag="POB34_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004149557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI5920481.1"
                     /translation="MKLLLLTGATGFLGGAVLDKLLDNCNNINLLLLVRAPTPQAGLE
                     RIKENMRKFNVCEERLHALTNDNILPGDLNNPEAFLMDPRLDEVTHVINCAAIASFGN
                     NPFIWNVNVTGTLAFARRMAKVAGLKRFLHVGTAMSCTPHTGSLVKEESASSETGEHL
                     VEYTHSKATIEYLMRKQCPDLPLLVARPSIIVGHSRLGCLPSTSIFWVFRMGLMLQKF
                     MCSLDDKIDVIPVDYCADALLMLLESSLINGEIVHISAGKESSVTFSAIDEAVARALN
                     CDPVGDRYTKVSYDILAMSRHDFKNIFGPCNERLMLKAIRLYGAFSMLNVCFSNDKLL
                     SIGMPKPPKFTDYIKYCIETTKHLSIQQQMEVDFK"
     gene            complement(7111..8064)
                     /locus_tag="POB34_003606"
     CDS             complement(7111..8064)
                     /locus_tag="POB34_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HDI5920482.1"
                     /translation="MYLKILATALSAPVAFAALASDTGLSFTPEKISTEIDFGTLSGK
                     AKERVYLPEEKGRKASQLDWKYSNAPIVKGAFNWDLLPRVSVGASGWTTLAGRGGNMV
                     DRDWLDTSNPGTWTDESKHPNTRLNFANEFDLNIKGWLLNQPDYQLGLMAGYQENRYS
                     FTAKGGSYIYSSEGGFRDETGSFPDGERAIGYKQHFKMPYIGLTGNYRYDSFEFGGSF
                     KYSGWVKASDNDEHYNPEKRITYRSDVNNQNYYSVSLHAGYYITPAAKVYVEGTWNRI
                     TNKKGDTSLYSRNLNISDHTKNGAGIESYNFMTTAGLKYYF"
     gene            complement(9337..9495)
                     /locus_tag="POB34_003607"
     CDS             complement(9337..9495)
                     /locus_tag="POB34_003607"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI5920483.1"
                     /translation="MAQNAFIESFNRTYRTKILGFCLFRTLDEKRELAANWLSEYNSE
                     RHINYLTI"
     gene            9679..10892
                     /locus_tag="POB34_003608"
     CDS             join(9679..9970,9970..10892)
                     /locus_tag="POB34_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDI5920484.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQGEYDSQWAAICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTSAERQRLKELERENRELRRSNDILRQASAYFGEGGV
                     RPPLEKMMPLLDKLREQYGVGPLCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLK
                     KEIQRVYDENHKVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTIS
                     RKAVVAGDRVNRQFVAERPDQLWVADSTYVSTWQGVVYVAFIIDVFAGYIVGWRVSSS
                     METTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYD
                     NAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKA
                     YYASIGNDDLAA"
     misc_feature    9960..10076
                     /locus_tag="POB34_003608"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            complement(11040..11308)
                     /locus_tag="POB34_003609"
                     /pseudo
     CDS             complement(11040..11308)
                     /locus_tag="POB34_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3129 a         2538 c         2415 g         3226 t
ORIGIN      
        1 gctttttcgg ttgaaaagaa attgattgaa ttactggaaa aacaatctgg tctccatacg
       61 tctgtggatt gcagtactcc ccttacagag cagagtctgt cgagaaatga cggtgaagac
      121 cagaccagaa gaaacgctga aaatatgttc ggagatgact gaactaaccc agcctggcca
      181 ggggaatatc tcgccattta gttgtataac actgactcaa catttcccgt ttcatttgcc
      241 attcaggggc ggttcctcgc cccgcaaacc acactttccc ttttcccgac tggttcagtt
      301 catcgagtgt tttcattaac ttttcactgt ttttacgggg ctggatttca tcaaataatc
      361 ccggctgcgc tatacccgat ggtgtgaaat cagccagcat gactcctgcc ttcatatagc
      421 ggtacccttc acgccagaca tggtttaagg ctctgcatgc ggcggcaata atgtcccggc
      481 tgtcctgtgt gggcaatgaa agcttttcca cagcggcatt gctgtaacag ggttcagggg
      541 gatgtccgta caaatgtcgt cacctgccgg caatactgac gctccccacg tagtttctct
      601 gcggcccgct ctgcatactg aacaacagcc tggtgcatgg catctttgtc tgtgattcgt
      661 tcaccaaaac tgcgactatg tcaacgccac gatgtttgac cgttatttgc cattttcatg
      721 cccgctaaat actttcgtta ctgatgctgt ccgctttgca ccatttcatc atcactgatt
      781 ggcgttgtgt ttttggtcag cactccagct ttacgtttct cttttaaccg gtaactctct
      841 ccttgttctt tttgacggac gcattttacg tttgggctgg atgtagtaac gcagcatgga
      901 acgtccgccc gtataaccca ttgccttaat ctccgcgaag ataacctcgc tattccagac
      961 attctctgcc aggcgcatgt cgatgtaatc cataaacggt ttcagcttaa ccattttgtg
     1021 gcgggtcttt ctggctggcg gttcagggta tttgaggtag cgtctgaccg tccgttcaga
     1081 gcaaccaatc tgagccgcaa tatcgacaat gtacgcgccc tgctggcgca tttgctttat
     1141 catgtaaaag tcctctctgc tcagcatgtt gatgtccttt ctggtgtgag aaccttaagg
     1201 aaacaacatg ttgggtggag cggacaatcc aaatggtgaa ttaccgtctt atatcactgg
     1261 cgctgacacc tgtagtttgt atacacaatc cctggcattg aaacacacat atgaagtgta
     1321 cttcggatat gaccgtacgc tcaggaagta cggcagattc cgtcaaaaat tacctgtaaa
     1381 gacagacatc agaaaaaaca gtgctcgccg cagaccggtg accgggcgaa gcccgcgaac
     1441 cggcgaggaa gcgcaatgta tctgtagtta cagggagcag gtacgccctg aagccctttc
     1501 ataaaacggt tgtgtgcgct gtccttctgg aatgatttat ccacatatcc ggaggcccga
     1561 tccagtaaac agatccatga atgatcaaca aaggatccat taaagatccc catatcgctg
     1621 caaaccttgt cactcatggg ccgggagcac gatcacataa gctgtggcat gttactgata
     1681 aactgtaaca tgctaatgat aagctgtatt cagtaatcca tatactgaag taagttaatg
     1741 acataaacta tggtcagtat gccagactca gttgttaaat acaggctgca ggtttttctt
     1801 cagtcagtta gcggagttct gacacacgat tttctgttta ttcttttact gtccacaggc
     1861 tggaggcttt ctggaaaacg aaaattcaga catcaaaaaa ctgttcggcg aggtggataa
     1921 gtcctccggt gagctggtga cactgacacc aaacaataac aacaccgtac aacctgtggc
     1981 gctgatgcgt ctgggcgttt ttgtaccgac ccttaaatca ctgaagaaca gtaaaaaaaa
     2041 tacactgtca cgtactgatg ccacggaaga gctgacacgt ctttccctgg cccgtgctga
     2101 gggattcgat aaggttgaga tcaccggccc ccgcctggat atggataatg atttcaagac
     2161 ctgggtgggg atcattcatt cctttgcccg ccataacgtg attggtgaca aagttgaact
     2221 gccttttgtt gagtttgcaa aactgtgtgg tataccttca agccagtcat cccgcaggct
     2281 gcgtgagcgc atcagccctt ccctgaagcg cattgccggt accgtgattt cgttttcccg
     2341 taccgatgag aagcacaccc gggaatatat cacccatctg gtacagtcag cttactacga
     2401 tactgagcgg gatattgtgc agttacaggc cgatccccgc ctgtttgaac tgtaccagtt
     2461 tgacagaaaa gtccttctcc agcttaaggc gattaatgcc ctgaagcgac gggagtccgc
     2521 ccaggcactc tacaccttta tagagagcct gccccgggat ccggcaccga tatcgctggc
     2581 gcggctgcgt gcacgcctca atctgaagtc tcctgtattt tcccagaacc agacggtcag
     2641 acgggcaatg gagcagttgc gcgagattgg atatcttgat tacacggaga tccagcgggg
     2701 gcggacaaaa ttcttctgta ttcactaccg gcgtccccgg ttaaaagcgc cgaatgacga
     2761 gagtaaggaa aatccgttgc aaccttcacc tgcggaaaaa gtcagtccgg agatggcgga
     2821 gaagcttgcc ctgcttgaga aactgggcat cacgctggat gacctggaaa aactcttcaa
     2881 atcccgctga acataaactg tagtcagtga agagcgctcc tttactgact acagcttata
     2941 ttaaccggtg cagtgagtgg tctgctcact gcagtttata ttctgtttcc tgcagtgctg
     3001 tctgtagctg agctgccatc tgcctgtccc ttacgtaagt gatcccgtaa cctgatgctg
     3061 aggcattgct cccttcataa aacatgactt actcactaca gcttatatac atgctccagc
     3121 ttatgttatg cctgttctgc tgaccacagc ttatataagg aagcgcgtat gccgtcacct
     3181 caggggaggc agtgtacgca ggatctccgc agcatcccgc ccgtcacctg tgaaaggcac
     3241 tgccagcgtg gcagccatat ccagtgcaaa cactctggta taaacctcca tcgaacgtgg
     3301 atccctgtga ccaaccagtg cctggatgac tttccggggc tggcggtgat agagcatgtg
     3361 catgatatag ctgtgccgga aggtgtgtgg tgtgaccgga atcgaaaagt gtactccgtc
     3421 agcttcggcc cgtctgacag cctgcttcag ccagttgcgc atggtttcgt cggtcacggc
     3481 ccataatggt tcacgacgac ggggccgggt ggtgatcatc cagctttcca tctgcctgac
     3541 atagcttata tctgtcagcg gaaccaggcg cacttcatct tttggcgggc gtccgcgtcg
     3601 cgcacgcact ttttcggaca ggatccgcac aaacggtctt actccattca ggtcaaatga
     3661 ttccggcgtc agcatccggg cttcgccaat acgcattccg gtattccaga gggtggcgaa
     3721 cagcatatgg tgacgctgat ccggcatata gaagagaagg gcactcactt ccggggccag
     3781 caggtaggcc ggtgtggccc ccgtggaagt ggccattctt ctcagggaga gcgctgttgc
     3841 aaaatccacc cccggcgcaa tgggtaacag ggagacgcgt tcaggtgaat tctgcagggg
     3901 aatgacattg ttcatgagtt atttttcacg tagcacacgc actgtttatg gatacaggta
     3961 ttctgacctg aacgcccgga tccttccacg acaataaatc gcagcgcgcg gatccttttg
     4021 tttgcttatc acgcattatt gcgggtaaaa ccaaatacgt aaaggcgcgt tttgccgcag
     4081 ggatcctgtt ttaagccagt catggtgcgg gctgaggact ggtttgccgt ttttgggaga
     4141 attggggtaa tgccaggaca ggcagaaacg gagaaaaaat cctgcagacg cccttaaaag
     4201 acaactgaaa atttctgact ccatattcct ctggtttcag actgcttaat cacatcatca
     4261 acctgataca taacaaacag attctgttca cagaatgaac aattaagtaa atggttagat
     4321 tatgcgaaaa agtacagata tttctcacca tcgttattga ataatatgtc ttcacacaaa
     4381 caatattaat attttgagtg taagccctga ctcaaagaaa gaaatgtctg ttttcggaat
     4441 caaaaatata tagttatcaa aatacattaa tttcaaattt gaaattaatg taaaacgatt
     4501 tagtaaatgt tatgatatta taaatctact cctccatgtt tagaggagta tttgcagact
     4561 ggacgctctt atatagttac ttacctttat acatattcta acataagcat ttaccaacct
     4621 tacagggaac agaccatagt agtttatatt tccattcagg tctgaatgca ccgatagaat
     4681 atatcatctt tttattatta tcctgacaat accttcgcgc tttatctata ttcagccaca
     4741 taaggacact tcccggactt agtttcatgc aattctcatc attcagaact gcaccatttg
     4801 gaacgtcata ataaacatta catgagcttt cagatttcag tactatatca atagcacacg
     4861 gtttattatc ccagaatagc acatggccaa acataagttc tcgtaagtga gaaataaaca
     4921 ttaacagatt atcatattcg tagcaaggta gggtacctcc aaaccgggaa tgaaaaagtg
     4981 atatataaga gctggatatt tctttatcgg ataactggct tatacactta atctctccgc
     5041 cattcctgat aaatctctgg atttcatttc ggcgagtttt ttcaaattta ggtgaaaaag
     5101 attcctttat aatacaattt tgtttttttc tggctatttt ccatgttgca ttgataatat
     5161 tttgtttatt tataattgat aatttattag ttttatccgg aaaaaaacat cttgcatgtg
     5221 gggaaaaagg aattaatacc tcatcagatg aaagaggata agaccttctt gccattatcc
     5281 caatttgttt tccattgcac agaaaatatg agcctttgat atcaccatct ttttcatagt
     5341 gaaaaaaatc aaacttccag tcatgatgta tcataaagta tgaaacaaca tccggatgca
     5401 tgtttacact accaccgtat ttttcaaaag tttttttgta cgtttcgaat gtagatggtt
     5461 tccaggccgt aataatgcgc tttatatttt taattgacaa agtcatatcc tcggcgtttt
     5521 tatatattat gagtgcaaca ccaacaataa ttctgattat gataaatatt atttaaaatc
     5581 aacttccatt tgttgttgaa ttgaaaggtg ttttgtcgtt tctatacagt atttaatata
     5641 atcagtaaac tttggcggtt taggcattcc gatactcagt agcttgtcgt tactgaaaca
     5701 aacattgagc atactgaacg ctccatataa acgaatggct tttaacataa ggcgttcgtt
     5761 acagggacca aaaatatttt taaaatcatg acggctcatt gccagtatgt cataactgac
     5821 tttagtatat ctgtctccaa caggatcaca gttcaaagca cgggctacag cttcgtcaat
     5881 agcagagaac gtcacactac tttctttacc tgctgatata tgaacaatct caccattaat
     5941 taacgagctt tcaagcaaca ttagcaatgc atcagcacaa taatctacag ggataacatc
     6001 tattttatca tccagagagc acataaattt ttgcaacatt aaccccattc tgaataccca
     6061 gaaaatactg gttgaaggta agcaccctaa acgactgtgg ccaacaataa ttgatggtcg
     6121 ggcaaccaac aaaggtaaat caggacactg cttacgcatc agatattcta ttgttgcttt
     6181 tgaatgcgta tactccacta aatgttcacc tgtttctgat gaagcagact cttccttaac
     6241 tagcgacccc gtatgaggtg tacaagacat agcagtacca acatgaagga agcgtttcag
     6301 tcctgccact tttgccattc ttcttgcaaa agcaagtgta cctgtaacat tcacattcca
     6361 tataaaagga ttattaccaa aagaagctat agccgcacag tttataacat gagtgacttc
     6421 atcaagacga ggatccatga gaaaggcttc cggattattt agatccccag gcaagatgtt
     6481 atcattagtt aatgcatgca acctttcctc acaaacatta aatttacgca tattttcttt
     6541 aattctttcc agtcccgctt gtggagtagg tgctcgtact aaaagtagca aatttatatt
     6601 attacagtta tccagcagct tatccaggac cgcgccacca agaaatcctg ttgcacctgt
     6661 aagtaataat aatttcatat ctaacctctt ttgttttcat gtgatcatta taaaaataaa
     6721 ttatcttaca taaacaatga ttaaacaaaa tcaagagaga ttaaatgtaa aaacaatgtt
     6781 tatttttata cggcagaatc cttcaggtta ttgcgtgtgt ttttattgga tggtcttctt
     6841 attattgacg agtaatagaa ttataataaa gttcatattg ttaacatcgc ttatcaatta
     6901 tttttatgtc gataacactg tgtcgttatg catgaagagt ggcagggcag catctttaaa
     6961 aatacaagag ttaacagaaa cacttttatc gttgagcaca tataccctcc acatattata
     7021 aaaaatcaaa agaccaccat cagaagtgct ggccttttgt ttatcatatt taaagtgtca
     7081 tgaaaaagtt aaaaacaggc atgcttcagg ttaaaaataa tacttcagac cagctgtggt
     7141 cataaagtta tagctttcaa tgcctgcacc atttttggtg tgatcagata tattcaaatt
     7201 tctggagtac agagaagtat cacctttctt attagtaata cggttccatg ttccttcgac
     7261 ataaacttta gctgcaggtg taatataata accagcatgt agtgagacag agtaatagtt
     7321 ctgattattt acatcgctac gataggtgat ccttttctca gggttgtaat gctcatcatt
     7381 atccgatgcc ttcacccatc cgctatattt gaaactcccc ccgaactcaa agctatcata
     7441 ccgataatta ccggttaacc ctatataggg cattttaaag tgttgtttgt agcctatagc
     7501 tctttcacca tctggaaatg atcccgtttc atcccggaag cctccctcac tactatagat
     7561 ataagagcct ccttttgctg taaagctata acgattttcc tggtaaccag ccataagacc
     7621 taactgatag tctggttgat tcaggagcca tcccttaata ttcaggtcga attcattagc
     7681 aaaatttaga cgggtattcg gatgtttact ctcatcagtc caggttccgg gattgctcgt
     7741 atccagccag tccctgtcca ccatattgcc tccacgacca gctaatgttg tccaccctga
     7801 ggcaccgaca gatactctgg gtaacagatc ccagttaaat gcccccttga caattggcgc
     7861 attactgtat ttccagtcca gctggctggc tttgcgccct ttttcttcag gcagataaac
     7921 ccgttctttg gccttgccac ttaaagtgcc aaaatcaatc tccgtactga ttttctccgg
     7981 tgtaaaagaa agcccagtat cagaagccag tgcagcaaac gctacaggag ctgagagtgc
     8041 agtagcaaga atctttaagt acatagacaa gttaactcca ttccactcgt ttgtttttga
     8101 taaatatttt tttatttact cgtagtagtc aactgaaaaa tgctgttttg tttaacgttt
     8161 gtttacaatg tggccacatg tcgctttatt tattcccttt tatgtcagaa tatcagggga
     8221 gtcagtcaaa cccggtatga ttactgcccc ggtcatccat gttccagaaa atatcagggc
     8281 atacagggcg ctggtaactg cgaataaggc cctggtcgga cactttatgc cgggaacgtc
     8341 tgtaacgtgg aaaccggtac cttcttcctg gggttatttt gttaactctt atctaaaggg
     8401 agacaaaatg gccaaatcct gcgattatga agcaatcagg gtctttatta gcgttaatgt
     8461 cggcaaatat accaataacg ccattacagt taattattca gatagcgttt gtgcgacaag
     8521 acattaccat tcgacgaaaa taaactcagg tggttgatat cagaaatcaa ccgtggctcc
     8581 acctacaaaa aaagttgctg gtttataaat ccctcctttc tgattaataa gaaaactaat
     8641 aacatatgag tgtgattact tgttcagata agttaattat cttagtgatt gatatttatt
     8701 ttgtttcagt gaaaatgatg cttttattag aaaatttata aataccaagt cggtggtgtt
     8761 tattagtgta atggagggga gtgcgtaaat cgttcatgta acacttcctt ccaataatac
     8821 tttataaatc aatagatgta tgattactta tttcctgaaa tatcatatat attgctatat
     8881 ttcttaacga gatattctta ttttattgat aattgcatct cttttattta atgtaaagct
     8941 ttcttattaa tcttctaatg tattgcaaaa tggtagatgc tgagtaatca acagacttgt
     9001 atgtaaagtc aagattaacg ttgctaaagc acgcatcaat tttctgataa aaggatgggg
     9061 ttgttttttt cgataaaaaa aattcttgtc taaccactgg tgaaataaga ttattattac
     9121 cccgaattaa tactgagcct aacaatagaa tacaaaaaaa acaaaatgct ttaaataagg
     9181 ggagcactat aagttatcct ctttacttta tgcttaccaa ctcatttatg ttactttata
     9241 acataaccca tcttgcgttg taaacagact cgttttagtt ccatgcacct tttgagatcg
     9301 tgaccaaata atggcgttag tgatgttctt ccagtgtcat atggttaagt agttgatatg
     9361 gcgttcactg ttatattctg acaaccagtt tgccgccagt tcccgctttt catccagcgt
     9421 tctgaacaga caaaaaccga gtatttttgt tcggtatgtc cggttaaaac tctcgataaa
     9481 agcgttctgc gccaacttac ctgattgggt aatctccagt tttactacat gtttttctgc
     9541 gcattacgtc cgtgttggat aaaaaaaatc ctggccataa tctatgcgaa tcatagctgg
     9601 atagacatgg ttttccgcaa tccttgaacc gccccgggaa tcctggagac taaactccct
     9661 gagaaagagg taaacaggat gactaaaaat actcgttttt cccccgaagt ccgtcagagg
     9721 gcgattcgta tggttctgga aagtcagggc gaatatgact cacagtgggc ggcaatttgt
     9781 tccattgccc caaagattgg ctgtacaccg gagactctgc gtgtctgggt acgccagcat
     9841 gagcgggata ccggaggcgg tgatggcggg ctcaccagcg ctgaacgtca gcgtctgaaa
     9901 gagctggaac gtgaaaatcg tgaactgcgc cgcagtaacg atatccttcg ccaggcttcc
     9961 gcttattttg cgaaggcgga gttcgaccgc ctctggaaaa aatgatgcca ctgctggata
    10021 agctgcgtga gcagtacggg gtcggaccgc tatgcagcga actgcatatt gccccgtcaa
    10081 cgtattacca ctgtcagcaa cagcgacatc atccggataa acgcagtgcc cgtgcgcagc
    10141 gcgatgactg gctgaagaaa gagatacagc gcgtatacga tgaaaatcac aaggtatacg
    10201 gtgtgcgtaa agtctggcgt cagttgttac gggaaggtat cagagtggcc agatgcactg
    10261 tggcacgtct catggcggtt atgggacttg ccggtgttct ccgtggtaaa aaggtccgta
    10321 cgaccatcag ccggaaagcc gttgtcgcag gcgaccgcgt aaaccgtcag ttcgtggcag
    10381 aacgtcctga ccagttgtgg gtggctgatt ctacttacgt cagcacatgg cagggggtcg
    10441 tctatgtggc gttcatcatt gatgtgtttg ccggatacat cgtggggtgg cgggtctcat
    10501 cgtctatgga aacgacattt gtgctggatg ctctggagca ggcgttatgg gcccgtcgac
    10561 cgtccggcac agtccatcac agtgataaag gttctcagta tgtatcgctg gcctacacac
    10621 agcggcttaa ggaagccgga ttactggcat caacaggaag tacaggcgac tcgtatgaca
    10681 acgcgatggc ggagagcatc aatggccttt acaaagcgga ggtaatacac cgtaagagct
    10741 ggaaaaaccg tgcagaagtg gaactggcca cactcacgtg ggtggactgg tataacaatc
    10801 gacgattgct ggaaaggctg ggccatactc ctccggcaga agcagaaaaa gcttattatg
    10861 cttccatcgg aaacgatgat ctggcagcct gagttcacag ataaaacact ctccaggaaa
    10921 cccggggcgg ttcacctaca gagtaccgct accacttgtg aagctggcag attcagattg
    10981 ttttcaatcg acaacgcctc atagttaaag tcatcaacga cattgaacgt gtgaagtggt
    11041 caacaaaaac tggccaccga gttagagttt ttccagtatc gattttccga ttcgtttggg
    11101 gataacccac cgttatattc gtgcggtctt agtgcgctgt aatatccaac gatatagacc
    11161 gttatggcgt gagcggcctc gcagaagctt cgtaacccac caccggcatc cattcgttct
    11221 tcagactcct gaagaagcgt tccattgggc tgttatccca gcagtttccg cgccggctca
    11281 tactctgcct gatctggtat cgccacaa
//
LOCUS       DAMZET010000136        11202 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.136, whole genome shotgun sequence.
ACCESSION   DAMZET010000136 DAMZET010000000
VERSION     DAMZET010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.136"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..2099
                     /gene="mdtC"
                     /locus_tag="POB34_003610"
     CDS             <1..2099
                     /gene="mdtC"
                     /locus_tag="POB34_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HDI5920485.1"
                     /translation="RSSLEEVEQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLI
                     GTFAAMYLCGFSLNNLSLMALTIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTR
                     EVGFTVLSMSLSLVAVFLPLLLMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCG
                     WMLKASKPREQKRLRGFGRMLVALQQGYGKSLNWVLNHTRLVGVVLLGTIALNIWLYI
                     SIPKTFFPEQDTGVLMGGIQADQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSR
                     VNSGMMFITLKPRDERSETAQQIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASY
                     QYTLLSDDLAALREWEPKIRKKLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGID
                     VQAANSLLNNAFGQRQISTIYQPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIP
                     LSYFAKWQPANAPLSVNHQGLSAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVR
                     GSFAGTAQVFQETMNSQVILIIAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALL
                     ALELFNAPFSLIALIGIMLLIGIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRF
                     RPIMMTTLAALFGALPLVLSGGDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFD
                     RLRLRFSRKPKQTVTE"
     gene            2100..3515
                     /locus_tag="POB34_003611"
     CDS             2100..3515
                     /locus_tag="POB34_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5920486.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAITGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            3512..4915
                     /gene="baeS"
                     /locus_tag="POB34_003612"
     CDS             3512..4915
                     /gene="baeS"
                     /locus_tag="POB34_003612"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HDI5920487.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            4912..5634
                     /gene="baeR"
                     /locus_tag="POB34_003613"
     CDS             4912..5634
                     /gene="baeR"
                     /locus_tag="POB34_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HDI5920488.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREHLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            5825..6157
                     /locus_tag="POB34_003614"
     CDS             5825..6157
                     /locus_tag="POB34_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HDI5920489.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            6366..6662
                     /locus_tag="POB34_003615"
     CDS             6366..6662
                     /locus_tag="POB34_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDI5920490.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            6664..6960
                     /locus_tag="POB34_003616"
     CDS             6664..6960
                     /locus_tag="POB34_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDI5920491.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            7063..8424
                     /gene="yegQ"
                     /locus_tag="POB34_003617"
     CDS             7063..8424
                     /gene="yegQ"
                     /locus_tag="POB34_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HDI5920492.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(8755..9072)
                     /locus_tag="POB34_003618"
     CDS             complement(8755..9072)
                     /locus_tag="POB34_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920493.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            9478..10377
                     /gene="yegS"
                     /locus_tag="POB34_003619"
     CDS             9478..10377
                     /gene="yegS"
                     /locus_tag="POB34_003619"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HDI5920494.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(10459..>11202)
                     /locus_tag="POB34_003620"
     CDS             complement(10459..>11202)
                     /locus_tag="POB34_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDI5920495.1"
                     /translation="IQLVNEQGTVLVQDLAGVFAASEATIRADLRFLEQKGVVTRFHG
                     GAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVILDSGSTTMLI
                     AEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGSIAERSLQDIN
                     ADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNRRGFNQVLPIE
                     KIDIIITDDAVSEVDKLALQKTRVKLITV"
BASE COUNT         2650 a         2845 c         2897 g         2810 t
ORIGIN      
        1 ttcgctcctc gctggaagaa gtcgagcaaa cgctgattat ctcggtggcg ctggtgattc
       61 tggtggtgtt tttattcctg cgctcgggtc gcgccactat tattcccgcc gtttcggtgc
      121 cggtttcgct gattggtacg tttgcggcga tgtacctgtg cggattcagt ctcaataacc
      181 tttcgttaat ggcgctcacc atcgctactg gtttcgtggt ggatgacgcc atcgtggtgc
      241 tggaaaacat tgcccgtcat ctggaagcgg gaatgaaacc gttgcaagcc gcactgcaag
      301 gtactcgcga agtcggtttt acggtgctgt cgatgagtct gtcactggtg gcggtgttcc
      361 tgccgctgct gttgatgggc ggattgccgg gccgactgtt acgcgagttt gccgtgacgc
      421 tttctgtcgc cattggtatt tcgttgctgg tttctctgac attaacgcca atgatgtgtg
      481 gctggatgct gaaagccagc aagccgcgcg agcaaaagcg actgcgtggt tttggtcgca
      541 tgttggtagc cctgcaacaa ggctacggca agtcactgaa ctgggtgctc aatcataccc
      601 gtctggtggg cgtggtgctg cttggcacca ttgcgctgaa tatctggctg tatatctcga
      661 tcccgaaaac cttcttcccg gagcaggaca ctggcgtgct gatgggcggg attcaggcgg
      721 atcagagtat ttcgtttcag gcgatgcgcg gtaagttgca ggatttcatg aaaattatcc
      781 gtgacgatcc ggcagtggat aatgtcaccg gctttacagg cggttcgcga gtgaacagcg
      841 ggatgatgtt tatcaccctc aagccacgcg acgaacgcag cgaaacggcg cagcaaatta
      901 tcgaccgtct gcgcgtaaaa ctggcgaaag aaccgggggc gaatctgttc ctgatggcgg
      961 tacaggatat tcgcgttggt gggcgtcagt cgaacgccag ctaccagtac acgttgttat
     1021 ccgacgacct ggcggcactg cgcgaatggg agccgaaaat ccgcaaaaaa ctggcgacgt
     1081 tgccggaact ggcggacgtg aactccgatc agcaggataa cggcgcggag atgaatctgg
     1141 tttacgaccg cgacaccatg gcacggctgg gaatcgacgt acaagccgcc aacagtctgt
     1201 taaataacgc cttcggtcag cggcaaatct cgaccattta ccagccgatg aaccagtata
     1261 aagtggtgat ggaagtggat ccgcgctata cccaggacat cagtgcgctg gaaaaaatgt
     1321 tcgttatcaa taacgaaggc aaagcgatcc cgctgtcgta tttcgctaaa tggcaaccgg
     1381 cgaatgcccc actatcggtg aatcatcagg gattatcggc ggcctcgacc atttcgttca
     1441 acctgccgac cggaaaatcg ctctcggacg ccagtgcggc gatcgatcgc gcaatgaccc
     1501 agcttggtgt gccttcgacg gtgcgcggca gttttgccgg aacagcgcag gtgttccagg
     1561 agacgatgaa ctcacaggtg atcctgatta tcgccgccat cgccacggtg tatatcgtgc
     1621 tgggtatcct ttacgagagt tatgtgcacc cgctgacgat tctctccacc ctgccctcgg
     1681 cgggtgttgg agcgctgctg gcgctggagc tgttcaatgc cccgttcagc ctaatcgccc
     1741 tgatagggat catgctatta atcggcatcg tgaagaaaaa cgccattatg atggtcgatt
     1801 ttgcgcttga agcccaacgg cacggtaacc tgacgccgca ggaagctatt ttccaggcct
     1861 gtctgctgcg ttttcgcccg attatgatga ctaccctggc ggcgctgttt ggcgcgctgc
     1921 cgctggtgtt gtcgggcggc gacggctcgg agctgcggca acccctgggg atcaccattg
     1981 tcggcggact ggtaatgagc cagctcctta cgctgtatac cacgccggtg gtgtatctct
     2041 ttttcgaccg tctgcggctg cgtttttcgc gtaaacctaa acaaacggta accgagtaaa
     2101 tgacagatct tcccgacagc acccgttggc aattgtggat tgtggctttc ggcttcttta
     2161 tgcagtcgct ggacaccacc atcgtaaaca ccgcccttcc ctcaatggcg caaagcctcg
     2221 gggaaagtcc gttgcatatg cacatggtca ttgtctctta tgtgctgacc gtggcggtga
     2281 tgctgcccgc cagcggctgg ctggcggaca aagtcggcgt gcgcaatatt ttctttaccg
     2341 ccatcgtgct gtttactctc ggttcactgt tttgcgcgct ttccggcacg ctgaacgaac
     2401 tgttgctggc acgcgcgtta cagggcgttg gcggcgcgat gatggtgccg gtcggcagat
     2461 tgacggtgat gaaaatcgta ccgcgcgagc aatatatggc ggcgatgacc tttgtcacgt
     2521 tacccggtca ggtcggtccg ctgctcggtc cggcgctcgg cggtctgctg gtggagtacg
     2581 cgtcgtggca ctggatcttt ttgatcaaca ttccggtggg gattatcggt gcgatcgcca
     2641 cattgatgtt aatgccgaac tacaccatgc agacgcggcg ctttgatctt tccggctttt
     2701 tattgctggc ggttggcatg gcggtattaa ccctggcgct ggacggcagt aaaggtacag
     2761 gtttatcgcc gctggcgatt actggcctgg tcgcagttgg cgtggtggca ctggtgcttt
     2821 atctgctgca cgccagaaat aacaaccgcg ccctgttcag tctgaaactg ttccgcactc
     2881 gtaccttttc gctgggcctg gcggggagct ttgccggacg tattggcagt ggcatgttgc
     2941 cctttatgac accggttttc ctgcaaattg gcctcggttt ctcgccgttt catgccggac
     3001 tgatgatgat cccgatggtg cttggcagca tgggaatgaa gcgaattgtg gttcaggtgg
     3061 tgaatcgctt tggttatcgt cgggtactgg tagcgaccac gctgggtctg tcgctggtca
     3121 ccctgttgtt tatgactacc gctctgttgg gctggtacta cgttttgccg ttcgtcctgt
     3181 ttttacaagg aatggtcaac tcaacgcgtt tctcctccat gaacaccctg acgctgaaag
     3241 atctcccgga caatctggcg agcagcggaa acagcctgct gtcgatgatt atgcaattgt
     3301 cgatgagtat cggcgtcact atcgccgggc tgttgctggg actttttggt tcacagcatg
     3361 tcagcgtcga cagcggcacc acacaaaccg tctttatgta cacctggctt agcatggcgt
     3421 tgatcatcgc ccttccggcg ttcatctttg ccagagtgcc gaacgatacg catcaaaatg
     3481 tagctatttc gcggcgaaaa aggagcgcgc aatgaagttc tggcgacccg gtattaccgg
     3541 caaactgttt ctggcgattt tcgccacctg cattgtcttg ctgatcagta tgcactgggc
     3601 ggtgcgtatc agttttgagc gtggctttat tgattacatc aagcatggta atgaacaacg
     3661 attacaactg ttaagtgatg cgcttggcga gcagtatgcg cagcatggca actggcgctt
     3721 cctgcgcaac aatgatcgct ttgtctttca gatcctgcgt tcatttgaac acgataattc
     3781 ggaagataaa cccggcccgg gtatgccacc gcacggctgg cgtacccagt tctgggtggt
     3841 tgatcaaaac aacaaagtgc tggttggtcc gcgagcgccg attccacctg acggtacacg
     3901 gcgacccatt ctggtcaacg gtgcggaagt tggcgcggtg atcgcctccc ccgttgagcg
     3961 gttaacgcgc aatactgata tcaatttcga taaacaacag cggcaaacca gctggctgat
     4021 cgtcgccctg gcaacgttac tcgcggcgct cgccaccttt ctgctggcgc gcggtttgct
     4081 ggcaccggta aaacgacttg tcgatggcac gcacaaactg gcggcgggcg atttcactac
     4141 ccgcgtaacg cccaccagtg aagatgaact gggcaaactg gcgcaagact tcaaccagct
     4201 tgccagcacg ctggagaaaa accagcaaat gcggcgcgat tttatggccg atatttcaca
     4261 cgaactgcgt acgccattag cggtgctgcg cggtgaactg gaagccattc aagatggcgt
     4321 gcgcaaattc acgccggaga cggtggcgtc tttacaggcg gaagtcggta cactgactaa
     4381 actggttgac gatcttcatc agttgtcgat gtctgatgaa ggcgctctcg cctatcaaaa
     4441 agcaccggta gatttgatcc cactgctgga agtggcgggc ggcgcatttc gcgaacgatt
     4501 tgccagccgt ggcctgaaac tgcagttttc cctgccagac agtattaccg tatttggcga
     4561 tcgcgaccgt ttaatgcagt tattcaataa cttactcgaa aacagcctgc gctacactga
     4621 tagcggcggc agcctgaaaa tctctgccga gcagcacgac aaaacggtgc gcctgacctt
     4681 tgccgacagt gcgccaggtg tcagtgacga gcagctacaa aaattatttg aacgttttta
     4741 tcgcaccgaa ggttcccgca accgcgccag cggtggttcc gggctggggc tggcgatttg
     4801 cctgaacatt gttgaagcac ataatggccg cattattgct gcccattcgc cttttggcgg
     4861 ggtaagcatt acagtagagt taccgctgga acgggattta cagagagaag tatgaccgag
     4921 ttaccaatcg acgaaaacac accgcgtatt ttgatcgtgg aagatgaacc gaagctgggg
     4981 cagttgctca ttgattatct gcgtgctgcg agctatgcgc cgacgcttat cagccacggc
     5041 gatcaggtac tgccgtatgt gcgccagaca ccaccggatc tgatcctgtt agatctgatg
     5101 ctccctggca ccgatggcct gacgctgtgc cgggaaattc gtcgtttttc tgacattccg
     5161 atcgtgatgg tgacggcaaa aatcgaagag atcgatcgcc tgctggggct ggagattggc
     5221 gcagatgatt atatctgtaa gccgtacagc ccacgggaag tggtagcgcg cgtcaaaacc
     5281 attttgcgcc gttgcaaacc gcagcgcgag ttgcagcaac aggatgctga aagcccgttg
     5341 attatcgacg aaggtcgttt tcaggcttca tggcgcggta aaatgcttga cctgacgcct
     5401 gcggaatttc gtctgctgaa aacgctctct catgaaccag gaaaagtgtt ctcccgcgag
     5461 catttgctca atcatcttta tgacgactac cgcgtagtaa ccgaccgcac catcgacagc
     5521 cacattaaaa acctgcgccg caagctggaa tctctcgacg ccgaacagtc atttatccgc
     5581 gccgtttacg gcgttggtta ccgctgggaa gccgacgcct gccgcatcgt ttagttttag
     5641 cgacatgact ttgttagccg gagatggcgt tcagaccaga tccggcaaca ttatcccacg
     5701 catggtcagc aaactgacat tatctctccg ccctggcagg catcagaaaa tcgctcatac
     5761 tttaatcggt aaacagcacc tttagatgct gttttcgata cgcaatttca atcaaggagt
     5821 cattatggct ggttggtttg aactcagtaa gagcagtgat aatcagttcc ggtttgtgct
     5881 aaaagcgggc aatggtgaga ctatcctcac cagcgagctg tatacctcaa aagcctctgc
     5941 ggaaaagggc atcgcgtcgg tgcgtagcaa cagcccgcaa gaagaacgct atgagaaaaa
     6001 aacggcaagt aacggcaaat tctatttcaa tctgaaagcc gctaatcatc aaattatcgg
     6061 ctccagccag atgtacgcca ccgcgcaatc tcgtgaaacc ggaattgcct ccgttaaagc
     6121 caatggcaca agccagacgg tgaaagacaa tacgtaatta cgatgccggg cgtgggttgt
     6181 tggcgtccgg ctttatatcc aaataacgct tagttattaa taattaagag taaccgccgc
     6241 cattaaaata gtgggcactg acgagcactc gttgtgcttt ctttgtttac catcccatac
     6301 ttatcctcct gaacatgttc atgtgccaaa atatcttcta atactttcgt gacgctatcc
     6361 ggtttatgac atacactgat gtaagatttt tacttacagg cgggcgtaaa atggccagaa
     6421 cgatgacggt tgatctcggc gatgagttgc gtgaatttat cgagtcctta attgaatccg
     6481 gcgactatcg cacccaaagc gaagtgatcc gtgagtcact gcgattactt cgagaaaagc
     6541 aagcagagtc tcgcctgcaa gcgttacgtg acatgctcgc agaagggttg agtagtggtg
     6601 aagctcagcc gtgggaaaag gacgcgttct tacggaaggt gaaagcaggg ataaggaaat
     6661 aatgtgcgta ttattaaact catgccaaaa gccaatgagg atttagaagg catctggtat
     6721 tacagctatc accatttcgg cgaaccacaa gccgacagat atgtggaaca tctttcagac
     6781 gttctccaga ttctgagtaa caataatata ggtacgccca gacctgaatt aggcgaaggt
     6841 atttttgttc ttccttttga acgccacgtc atttatttcc tgcaatcacc aggggaaatt
     6901 atcgtcattc gtatccttaa ccaaaaccag gacgccacca gacatttaca ctggagctaa
     6961 acccgccgat tgcgctacaa tgcccgccct taaagtgggg gcactcccct aaccgcttca
     7021 tcaggtgaag cggatctgac ctgtcatcag aacgagagaa ttatgtttaa accggaactc
     7081 ctttccccgg cgggaacgct gaaaaatatg cgttacgctt tcgcttatgg cgcagatgct
     7141 gtttatgcgg gccagccgcg ttattccctg cgtgtgcgca acaacgaatt caaccacgaa
     7201 aatcttcagc tcggcatcaa tgaagcccac gcgctgggga aaaagtttta tgtcgtggtc
     7261 aacattgcac cgcacaacgc caagctgaaa acctttatcc gtgacctgaa accggtggtg
     7321 gaaatggggc cggatgcgct gattatgtcc gatccagggc tgattatgct ggtgcgtgag
     7381 cacttccctg aaatgccgat ccacctttcg gtgcaggcta acgccgtgaa ctgggcgacg
     7441 gtgaaattct ggcagcaaat gggcctgacc cgcgtgatcc tctctcgcga gctgtcgctg
     7501 gaagagattg aagagatccg caatcaggtg ccggatatgg agatcgagat cttcgttcac
     7561 ggcgcgctgt gcatggccta ctccggtcgc tgcctgctct ctggctatat caacaagcgc
     7621 gacccgaacc agggcacctg caccaacgcc tgccgctggg agtacaacgt ccaggaaggg
     7681 aaagaagatg atgttggcaa catcgtacac aagtacgagc cgattccggt gcaaaatgtt
     7741 gagccgacgc tgggtatcgg cgcaccaacc gacaaagtgt ttatgatcga agaggcccag
     7801 cgtccgggcg agtatatgac cgcgtttgaa gatgagcacg gcacttacat catgaactcg
     7861 aaagatctgc gcgccatcgc ccatgtagaa cgcctgacca aaatgggcgt gcattcgctg
     7921 aaaatcgaag gtcgtaccaa atctttctac tattgtgcac gcaccgcaca ggtttaccgc
     7981 aaagctatcg atgacgccgc tgcgggaaaa ccgttcgata ccagcctgct ggaaactctg
     8041 gaaggtctgg cgcatcgtgg ctataccgaa ggtttcctgc gtcgtcatac tcacgacgat
     8101 tatcagaact acgaatacgg ttattcagtt tctgaccgcc agcagtttgt tggtgagttt
     8161 accggtgagc gcaaggggga cctcgcggcg gtagcggtga aaaataaatt ctccgttggc
     8221 gacagccttg agctgatgac gccgcaaggc aacattaatt ttacccttga gcacatggaa
     8281 aacgccaaag gcgaagctat gccgatagca ccaggcgatg gttatactgt gtggctcccg
     8341 gtcccgcagg atcttgagct caattacgcg ctgctgatgc gtaatttctc cggggaaacc
     8401 acgcgtaatc cccacggtaa gtgattaatt tcgattattt ttcccggatg gaaaattctt
     8461 agaaaccgat cacatacagc tgcatttatt aaggttatca tccgtttcgc tgaaaaacat
     8521 aacccataaa atgctagctg taccaggaac cacctcctta gcctgtgtaa tctcccttac
     8581 acgggcttat ttttttatgc ataagcccta tctctggtaa ccgtcttcca ttgaccacat
     8641 cgatagaatc tcccttcata gcacgatgcc tttcacgtaa cggcatcgtg ctcgcacagg
     8701 ttccggctaa gcacaaccag aacgcgcatg tttgacgctt accaaaaaat attctcactc
     8761 tccacatttg aatgtcagac gagcgacgcc atgtaatcct gcaccttctg tcttcaggtc
     8821 aactatctgc atttttttgc cctgagtaac acagaaatgg gctgcatcat tttttactat
     8881 attttctgca ccagatattc tgcccctggc taaagaagct tcggcttcgg tgtagtattg
     8941 attatcgagt ttacgctgaa tattactttt atatgcaaga ccaaatttac cgatacttgt
     9001 ctcatcatta tgcacagcac aaccagacat aataaaaata ctaattaatg atatagcagc
     9061 tatctttttc atctcacctt cccccattaa ataccaacga cactctctag tgtttaaata
     9121 taataatggc atgattatta taattgaata ggattataat aaatgttctg tacaacattt
     9181 cctacataag taggaattac ggacattgag gcccttcagg gtaactccat gggggcttta
     9241 atatattata ttgaagatgc cactgtttag ttgaatatta ggtatatgct cttttttgaa
     9301 atttatcggt ggcagccgtt agtattcgct gtccccattg caagctcctg gtggtaacca
     9361 ctgaatcctc catacttgaa ctgacttttt atcctccgac tttcatcctg ttctgactcc
     9421 accttttgtt ttctgctcta cactatctac agaccaatca taaaggcaca tacgatcatg
     9481 gcagaatttc ccgccagctt actgattctt aatggcaaaa gtactgacaa tctacccttg
     9541 cgcgaagcaa ttatgctgtt gcgtgaggaa ggaatgacga tccatgtgcg ggtcacctgg
     9601 gagaaaggcg atgccgcacg atatgtagag gaggcccgga agttgggcgt cgcaacggtg
     9661 attgccggtg gtggcgatgg caccattaat gaagtttcta cggcgttgat tcagtgtgag
     9721 ggggatgaca tacccgcgct gggaattttg ccattaggaa ccgccaatga ttttgccacc
     9781 agtgtaggga ttcctgaggc actggataag gcgctgaaac tggcaattgc cggtaacgcc
     9841 attgcgatag atatggcgca ggtcaacaaa caaacctgtt ttattaatat ggcgacaggc
     9901 ggatttggga cgcgtattac cacagaaacg ccggaaaaat taaaagccgc gctgggtggc
     9961 gtctcttaca tcattcatgg cttaatgcgc atggatactc tgcaaccgga ccgttgtgaa
    10021 atccgcggtg aaaactttca ctggcaaggt gacgccctgg tcattggtat tggtaacggg
    10081 cgtcaggccg gtggcggtca gcaattgtgt ccgaacgcgt taattaacga tggcttgctg
    10141 caactgcgca tttttaccgg cgatgaaatt cttccggctc tcgtatcaac cttaaaatct
    10201 gacgaagata acccgaatat tatcgaaggc gcttcgtcgt ggtttgatat acaagcccca
    10261 cacgaaatca cttttaatct tgatggcgaa ccgttgagcg gacaaaactt tcatattgaa
    10321 atacttccgg cggcgttgcg ttgtcgatta ccaccagatt gtccattatt gcgttaatca
    10381 aactattctc tcttgaacca gggagaatcc gaaatgccgg atgcggtgta aataccttat
    10441 ccggcataca gaacaatact acaccgtaat taattttacc cgtgtcttct gcaatgccag
    10501 tttatccacc tctgatacag catcatcggt aataataatg tcaatttttt cgattggcag
    10561 tacctgatta aaaccgcgac ggttaaattt cgatgaatcg agaacggcaa tcactttatt
    10621 agcggcggtg accatcgcac cgctaatcga ataaccttca ttaaaggtcg taataccatt
    10681 aaccgcatca ataccatcag caccgacaaa cattaaatcg gcattaatat cctgcaggga
    10741 acgttcagca atagatccat gcatagatcg cgttttatgg cggaccgtgc caccgcagac
    10801 gaccagagta atgtctttat tttcggaaag ggcaaacgca gccgggagac tgttggtaat
    10861 caccgtgata tttttagcgg tcattaatcc ttcggcgata agcattgtgg tacttccgct
    10921 gtcgagaata acggtcatcc cttcatggat cattttgact gccgcctgcg ctattctgtt
    10981 tttgggcgcg ctggcgagct gaaatcgctc tttaaacccg acttcctggg tctcggtttc
    11041 actattaccg gacattattt tcgccgcacc gccatgaaag cgcgtaacca cgcctttttg
    11101 ttcgagaaag cgcaaatcgg cacggattgt cgcttccgag gcagcaaata ctcccgccag
    11161 atcctgaaca agcacggttc cctgttcatt cactaattgg at
//
LOCUS       DAMZET010000137        11114 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.137, whole genome shotgun sequence.
ACCESSION   DAMZET010000137 DAMZET010000000
VERSION     DAMZET010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.137"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(166..1266)
                     /gene="chaA"
                     /locus_tag="POB34_003621"
     CDS             complement(166..1266)
                     /gene="chaA"
                     /locus_tag="POB34_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HDI5920496.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1536..1766
                     /gene="chaB"
                     /locus_tag="POB34_003622"
     CDS             1536..1766
                     /gene="chaB"
                     /locus_tag="POB34_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HDI5920497.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            1924..2619
                     /gene="chaC"
                     /locus_tag="POB34_003623"
     CDS             1924..2619
                     /gene="chaC"
                     /locus_tag="POB34_003623"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HDI5920498.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(2663..3016)
                     /gene="ychN"
                     /locus_tag="POB34_003624"
     CDS             complement(2663..3016)
                     /gene="ychN"
                     /locus_tag="POB34_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HDI5920499.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3201..4595
                     /gene="ychO"
                     /locus_tag="POB34_003625"
     CDS             3201..4595
                     /gene="ychO"
                     /locus_tag="POB34_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HDI5920500.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASIDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(4596..5246)
                     /gene="narL"
                     /locus_tag="POB34_003626"
     CDS             complement(4596..5246)
                     /gene="narL"
                     /locus_tag="POB34_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HDI5920501.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5239..7035)
                     /gene="narX"
                     /locus_tag="POB34_003627"
     CDS             complement(5239..7035)
                     /gene="narX"
                     /locus_tag="POB34_003627"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HDI5920502.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            7374..8765
                     /gene="narK"
                     /locus_tag="POB34_003628"
     CDS             7374..8765
                     /gene="narK"
                     /locus_tag="POB34_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HDI5920503.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            9158..>11114
                     /locus_tag="POB34_003629"
     CDS             9158..>11114
                     /locus_tag="POB34_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HDI5920504.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAER"
BASE COUNT         2702 a         2752 c         2888 g         2772 t
ORIGIN      
        1 aagagtacat tccggctttt caacagctgt tgcagtggtt tcgtgcaggt tgtggtacag
       61 gctcgcaatt ctgattaacg acctgtccag gtagtatgaa gaaatacaat aagcaggaat
      121 ttatattatc tcccccgatt tccgtcatcg gaggagatat aactgtcagg caaatatcgt
      181 catcaaatag gcggcaaaca gtgccagatg cgctgcgcca ttgagcacgt tagtacgtcc
      241 ggtggagaag gagatatggc acagcactaa agaggccacc atcaccacca tttctggcgc
      301 accaagtgca aactgtaatt cgttacccgt cataaaggca attagcgtga cgacaggtac
      361 ggtaagcgaa atggttgcta acactgaacc aaagaacaga ttcatcgcgc gctggacctg
      421 gttgttcaac actgctttta acgcacctaa accttccggc gacagaatca acagagccac
      481 caggaagcca gtaaaggcga caggggcatt catgctgtcg agcaatgtct ccagcgggct
      541 ggcattcatt ttggtcaccg caataacggc aatcagatgg ataatcaacc agatagcatg
      601 ccacacgctg ctatgggcag acggtttacc gtgatgcggg tcatcgtcat cactgtcatc
      661 ttcgtgctcg tagacaaaca aactttgatg cgttttggtc tggatcagca aaaatacgcc
      721 atacattgcc gcagaaatta atgctaccag taatgcctga ccggttgaaa aattcgccgc
      781 aggcagagcc attggaaata ccagtacgat tatcgccagg gggaacagcg caattaaata
      841 ctgcttgata ccaaacagat tcatatattg ggtggcaaac ttacgaccgc ccaacaataa
      901 tgaaaagcca accagcccac cggtaacaat cataatgatt gaatagagcg tatcacgcat
      961 tagcgttggc gcggcgtcgc cggttgccat taaagctgaa atcaaactga cttcaagaat
     1021 aaccactgaa aggctaagaa taagcgaacc gtaaggttct cccaggcgat gggctaatac
     1081 gtccgcatga cggacaacac taaaggcgct acttaaaata ccaataagcg caagaagatt
     1141 gatggcaatg accactggta gtgtctggct gcttccccac aggaacagca ctaccagcgc
     1201 cagaaccggg aaaataagcg aagtctcctt gtggcgggtt tttaccgcct cttgagcatt
     1261 tgacattatg gttatccctt tgcagatgaa tttatcgaaa atgtaaaaaa taggtaggaa
     1321 aaataacaga aagtgtctgg atatcggtaa cattttacga atttttaccc cactgtcgat
     1381 tttttactca ttgggacata aaaataagta ttacgtttag acaatgtttg ttttagcgtt
     1441 tcttaagaac agtctgacat gaaaattctt atgttttggc aagagtagat attgttgacc
     1501 acacttaatg ttcaactttg taaaaggagt caacgatgcc gtataaaacg aaaagcgatc
     1561 tgccggaaag cgtaaagcac gttctaccgt ctcatgccca ggatatctat aaagaagcgt
     1621 tcaacagcgc atgggatcaa tataaagata aagacgatcg gcgtgatgac gccagtcgcg
     1681 aagaaaccgc gcataaagtg gcctgggctg ctgtgaagca tgaatatgcc aaaggggatg
     1741 atgataaatg gcataaaaaa tcgtaaaacc ggtagcttag ttaaagctat tcgtgcggtg
     1801 ttgccttgca agtggtccgt ggattgcata ttgtcccgtt agtggtttca aaatgagcag
     1861 taaaaatgtc cggaagacac caaaaagttg tcgcagggaa gtatgcagtg gcggaggtgt
     1921 aaggtgataa cgcgtgattt cttgatgaat gccgattgta aaacggcatt tggtgccatt
     1981 gaagaatcac tcttatggtc agcagagcaa cgggcggctt cgctggcggc gacgctggct
     2041 tgtcgacctg atgagggacc ggtgtggatc ttcggttatg gatcgttgat gtggaatccg
     2101 gcactggagt ttaccgagtc gtgcaccggt acactggttg gatggcatcg cgcgttttgc
     2161 ctgcgcctga ccgccgggcg cgggactgcg caccagccgg gacggatgct tgcactgaaa
     2221 gagggcggac gcactacagg cgtcgcctat cgactgccag aagagacgct ggagcaggaa
     2281 ctaaccctgt tgtggaagcg cgagatgatt accggctgtt atctgccaac ctggtgtcag
     2341 cttgatcttg atgatggatg cacagtaaac gccattgtgt ttattatgga cccgcgacat
     2401 ccagaatatg aatctgacac tcgcgctcag gtcatcgcgc cgttgattgc ggcggcgagc
     2461 ggtccgctgg gaaccaacgc acaatacctg ttttcactgg aacaggagct tatcaaactg
     2521 ggaatgcagg acgatgggct gaatgatttg ctggtatcgg taaaaaaact gctggcggag
     2581 aattatccgg atggtgtgtt acggccggga ttcgcctgag taaacttccc tcatagtggg
     2641 gcgtcagacg ccccctcaaa cattaaaatg tgagcacttt atcggctgac agcgtccatt
     2701 gcgccagttc cacaagagta ccgatttcca ccccatcaat aagaggaagt gtgctaatcc
     2761 cgcgcccgtc ggtacaggtt ttgcacaatt tcaccggtac attctgagcg gtaaggatct
     2821 ccagcatttg ctgaatgttg tagccttccc ctggtttttg cccgcgcaac ccggctgtga
     2881 ccgcatcaga catcaggaac agacgcagat ccagattgct ctcctgctct cgtaacgcaa
     2941 tggccagccg caagctgtta aacaaggatt cgctcccgta aggtgcgcca ttggcaacga
     3001 tcacgatttt ttgcattatt tactcctgta ttcagggaat tagacattca tcttctatct
     3061 tactgcttct gcagcgtctg accaatcggt cacattttta aggattttcc tgaaagcgcg
     3121 agaaaatacg acaaaagttg ccagtaatcg ttattcttta aggctatggt ttttcattat
     3181 taccggaagt taccgacgtt ttgagccgtt tcgttcctcg cattatcccg ttttatttac
     3241 tcttgcttgt agcaggcggt acagctaacg cacaatctac cttcgagcaa aaagcggcaa
     3301 atccctttga taataacaat gatggtctgc cggatttagg catggctccc gaaaatcatg
     3361 atggggaaaa acactttgct gaaattgtga aagatttcgg cgaaaccagt atgaatgata
     3421 acgggctgga tactggcgag caggcaaaag ctttcgcatt gggaaaagtc cgcgacgcgc
     3481 ttagtcaaca ggttaatcag cacgtagagt cctggctatc accgtgggga aatgccagta
     3541 ttgacgtcaa agtggataac gaaggacatt tcaccggcag tcgtggaagc tggtttgtgc
     3601 cgttacaaga taatgatcgt tatctcacct ggagccagct tggtcttact cagcaggatg
     3661 atgggttggt gagcaatgtg ggcgttgggc aacgctgggc gcgcggcaac tggctggtgg
     3721 gttataacac tttttatgac aacttgctgg acgaaaatct tcagcgagcg ggctttggtg
     3781 ccgaagcgtg gggcgaatat ttgcgactat cggcaaactt ttatcagccg tttgctgcat
     3841 ggcatgaaca gacagccacg caggaacaac ggatggcgcg cgggtatgac ctgacagccc
     3901 ggatgcgcat gccgttctat caacacctca ataccagtgt cagcgtagaa cagtattttg
     3961 gtgagcgtgt tgatttgttt aactctggta cgggttatca caatcccgtc gcgttgagtc
     4021 tgggattaaa ttacacccct gtgccattag tcactgtgac ggcccagcat aaacagggtg
     4081 aaagtggcga gaatcaaaat aacctcgggc tgaatcttaa ttaccgcttt ggtgtaccgc
     4141 tcaaaaaaca actttctgcg ggcgaggttg ccgaaagtca gtcgttacgt ggtagtcgct
     4201 atgataatcc gcagcgaaat aatctaccga ctcttgagta ccgacagcga aaaacgttaa
     4261 cggtgtttct ggcgacaccg ccgtgggatc taaaacctgg cgaaacagtg ccgctgaaat
     4321 tacaaatccg cagtcgttat ggtattcggc aactgatttg gcagggcgat acgcagatat
     4381 taagtttgac gccaggcgca caagccaaca gcgcggaggg ctggacgctg atcatgcctg
     4441 actggcagaa cggggaaggg gcgagcaatc actggcgatt gtcggtggtg gtggaagata
     4501 accaggggca gcgtgtctcc tccaatgaga tcacgctaac gcttgtcgaa ccgttcgacg
     4561 cattgtcaaa cgacgaactg cgctgggaac cgtaatcaga aaatgcgctc ctgatgcacc
     4621 cataccgctg cttccacgcg agacttgagc ttcattttct tcagcatgtg cttgacgtgc
     4681 acttttactg tgctttcggt gatgtccagg cggcgggcaa tcatcttgtt cggcaaaccc
     4741 tgggcaatca gcttgagaat atcgcgctcg cgtggggtta actggttaac atcgcgttca
     4801 gtagtggcac ggttagcgcg caagctggct gccagaacag gcgttaatgc ctcgcttaat
     4861 accatttcgc cagcagctgc ctgatgcaat gctttcagca gatcttccgg ttccatatct
     4921 tttaatagat agccatccgc gccgcgtttc agtgcggtga ccacatcttc ttcatggtta
     4981 gagacgctga ataccacaat gcgccctgag agggactttt cgcgcagttt atccagcgtt
     5041 tccagaccgt tcatgccggg catattgaga tctaacagga tcagatcggg atcaagagat
     5101 tccgccagtt caataccctg ttcgccatta ctcgcttcgc caactacggt gatatctggt
     5161 gccatactga taagctgttt cacgccagtt cgcagcatcg gatgatcgtc gatcagcagg
     5221 atagtagccg gttcctgatt actcatgggt atctccttgg acgtctgtga aagttttttc
     5281 gggaataaag gtgacaacca cttcggtgcc acctgattca cgacggcgaa cgcggcaatc
     5341 gcctcgtaaa ctttgcgcac ggtcgcgcat tattatcatg ccgtagtgat tgctgcggat
     5401 ggcattttca ggcacgccgc agccgttatc ctggacggtc agtttgacct gattatcgtt
     5461 ttgcgccacc gtcaccacga cctcactcgc ttgcgaatgt ttgagggcgt tacttaatgc
     5521 ctcacgggca atttgcaaca agtggattgc ctgatgcgaa ggcaccagac gaggcggcaa
     5581 ttgataatcc agcttcaccg ggaagccaaa tttggcgctg tactcttcgc aactcgcctc
     5641 cagcgccgga cgtaatccag gctcggtgag ctgcaagcgg aatgtggtga gcaattcacg
     5701 caactgcgcc caggatgcat tcagttcgtt acggatctga cttaacagtt cgcggctgct
     5761 ttctggcagc gcatcgccct gcatctgtaa acaactcacc tgcatcttca tgcaagagag
     5821 agattgggca atagaatcat gcagttcgcg cgcaatggtg gcacgctctt ccatcacgat
     5881 caactgttgc tgacgttcct gatgccgatc cagcgccagc gtggcggtga gttgttcaac
     5941 cagggtatcc accagttgtt gttgatcatg gctaagatga cgcccctgcg gcagggtcgc
     6001 cagcaaaata ccgtactgcg tatgagagtc agccagccgc cacttcaggg tcgtgccgcg
     6061 atcaccaacg ggtaatacgc cgcgcgggca gagctggcag cctttatcat cacaagtcat
     6121 atctggctgg caggtaaact cctgatgatt ctcttcatca tcagtgtcat acacccgcag
     6181 ttcgatatca cgtagcaggg ttaaattctg taagccgttg agtacaggtg acaggcgttc
     6241 acacagcggg gcgcgggaat gcaaacggcg gttagcctgc cataaaaaag agaggatctg
     6301 atttttatgc tccagcccgg cggttttctc ctgaacccgc tgctcaagta cggcataact
     6361 ttcggccagt tctgcagaca tattgttcaa cgcagttcca agcatcgcca tttcgttgcg
     6421 cccgctgatg tttgcgcgtt gggtaaaatc gcgatggctg acggcactcg ccattgccag
     6481 cagttgccgc cacggttgta gcagtcgcgc ccgcaaccag ataatagtga acaccagtaa
     6541 aagtgccata aataccgcca ttacccgatg gaccagtacc actgtctcaa tgcgcatttc
     6601 cgtggtgcgg tcaaaaccag ataccagctg atcaagcccg gcaacaaact ggctgacatc
     6661 cgctgacacc gtttctcggt tttgtgcacg catcagcgca gggatcagtt cattacgcca
     6721 gtaatcttgt aaaccctgta attgcgccag ttgtccgtct cgttctgctg ctcgagtcaa
     6781 ctcggcgcta aatgccgttt gttccatctc tttaattaag ggcttgtctt tctcgcttaa
     6841 tggcactgcc gccaacagac ggtaactttg catgcgcagc gatcccgctt tgttgatcgc
     6901 atgggcgctg ccctgaacgc cttgcaccag ccagccagaa accgccatcc ctgccagtcc
     6961 aatagcagta gaaagcaaca caataagcgc aacctgatta accagggtga gcggagagag
     7021 acaacgttta agcatgtaaa cctcttcctt caggctttaa atgagcaata accttaatga
     7081 atgtgacgat acaatctgga atggcagtat tctcggctat tggctgaagt atacccatac
     7141 ccggaaagag ttactcctta tttgccgtgt ggttagtcgc tttacatcgg taagggtagg
     7201 gattctacag caccgtgaaa aatctcataa tttttatgaa atcactgtac tcactatggg
     7261 taatgataaa tatcaatgat agataaagtt atcttatcgt ttgatttaca tcaaattgcc
     7321 tttagctaca gacactaagg tggcagacat cgaaacgagt atcagaggtg tctatgagtc
     7381 actcatccgc ccccgaaagg gctactggag ctgtcattac agattggcga ccggaagatc
     7441 ctgcgttctg gcaacaacgc ggtcaacgta ttgccagccg caacctgtgg atttccgttc
     7501 cctgtctgct gctggcgttt tgcgtatgga tgttgttcag cgctgttgcg gtgaacttac
     7561 cgaaagtcgg ttttaatttt acgaccgatc agctatttat gttgactgcg ctgccttcgg
     7621 tttctggcgc gttattacgt gttccatact cctttatggt tcctatcttc ggtggtcgtc
     7681 gctggacggc gttcagcacc ggtattctga ttattccttg tgtctggctg ggttttgccg
     7741 tgcaggatac ctccacgcct tatagcgtct tcatcatcat ctctctgctg tgcggctttg
     7801 ctggcgcgaa cttcgcttcc agtatggcaa acatcagctt cttctttccg aaacagaagc
     7861 agggtggcgc gctgggtctg aatggtggtc tgggcaacat gggcgtcagc gtcatgcagt
     7921 tggttgctcc gctggtggta tcactgtcga ttttcgcagt atttggtagc cagggcgtca
     7981 aacagccgga tgggactgag ctgtatctgg cgaatgcgtc ctggatatgg gtgccgttcc
     8041 ttgccatctt caccattgcg gcgtggtttg gcatgaacga tcttgctacc tcgaaagcct
     8101 ccatcaagga gcagttgccg gtactcaaac ggggtcatct gtggattatg agcctgctgt
     8161 atctggcaac cttcggctcc tttatcggct tctccgcggg ctttgcgatg ctgtcaaaaa
     8221 cgcagttccc ggatgttcag attctgcaat acgctttctt cgggccgttt attggtgcgc
     8281 tggcgcgttc tgcaggtggt gcattatctg accgtctggg cggaactcgt gtcacgctgg
     8341 tgaactttat tctgatggcg attttcagcg gcctgctgtt cctgacctta ccgactgacg
     8401 ggcagggcgg aagcttcatg gcgttcttcg cggtcttcct ggcgctgttc ctgacagctg
     8461 ggctgggtag tggttccact ttccagatga tttcagtgat cttccgtaaa ctgacaatgg
     8521 atcgcgtgaa agcagaaggg ggttctgacg aacgtgcgat gcgtgaagcg gcaaccgaca
     8581 cggcggcggc gctgggtttc atctctgcga ttggcgcgat tggtggcttc tttatcccga
     8641 aagcgtttgg tagctcgctg gcattaacgg gttcgccagt cggcgcaatg aaggtatttt
     8701 tgattttcta tatcgcctgc gtagtgatta cctgggcggt atatggtcgg cattctaaaa
     8761 aataaatctt aatatttgat tatcctttgc gcggcatgat gtcgcgcttt ttttatgcgt
     8821 catttagtta caacatacta atgttatatg gtttatttcg ccggatttca ttaagagcca
     8881 ttaatatgtt acccatgggg aatactcctt aatacccacc tgcataaaat tcttaatagt
     8941 ttaaataact acaggtgtaa aacgtcttaa tttacagtct gttatgctgt ggctgttaat
     9001 tatcctaaag gggtatctta ggaatttact ttatttttca tccccatcac tcttgatcgt
     9061 tatcaattcc cacgctgttt cagagcgtta ccttgccctt aaacattagc aatgtcgatt
     9121 tatcagaggg ccgacaggct cccacaggag aaaaccgatg agtaaattcc tggaccggtt
     9181 tcgctacttc aaacagaagg gtgaaacctt tgccgatggg catggccagc ttctcaatac
     9241 caaccgtgac tgggaggatg gatatcgcca gcgttggcag catgacaaaa tcgtccgctc
     9301 tacccacggg gtaaactgca ccggctcctg cagctggaaa atctacgtca aaaacggtct
     9361 ggtcacctgg gaaacccagc agactgacta tccgcgtacc cgtccggatc tgccaaacca
     9421 tgaacctcgc ggctgcccgc gcggtgccag ctactcctgg tatctttaca gtgccaaccg
     9481 cctgaaatac ccgatgatgc gcaaacgcct gatgaaaatg tggcgtgaag cgaaggcgct
     9541 gcatagcgat ccggttgagg catgggcttc tatcattgaa gacgccgata aagcgaaaag
     9601 ctttaagcag gcgcgtggac gcggtggttt tgttcgttct tcctggcagg aggtgaacga
     9661 actgatcgcc gcatctaacg tttacaccat caaaaactac ggcccggacc gtgttgctgg
     9721 tttctcgcca attccggcaa tgtcgatggt ttcttacgca tcgggtgcac gctatctctc
     9781 gctgattggc ggtacttgct taagcttcta cgactggtac tgcgacctgc ctcctgcgtc
     9841 tccgcaaacc tggggcgagc aaactgacgt accggaatct gctgactggt acaactccag
     9901 ctacatcatc gcctggggct caaacgtgcc gcagacgcgt accccggatg ctcacttctt
     9961 tactgaagtg cgttacaaag gcaccaaaac tgttgccgtc acaccagact acgctgaaat
    10021 cgccaaactg tgcgatctgt ggctggcacc gaaacagggc accgatgcag caatggcgct
    10081 ggcgatgggc cacgtaatgc tgcgtgaatt ccacctcgac aacccaagcc agtatttcac
    10141 cgactatgtg cgtcgctaca ccgacatgcc gatgctggtg atgctggaag aacgcgacgg
    10201 ttactacgct gcaggtcgta tgctgcgcgc tgctgatctg gttgatgcgc tgggccagga
    10261 aaacaatccg gaatggaaaa ctgtcgcctt taataccaat ggcgaaatgg ttgcgccgaa
    10321 cggttctatt ggcttccgct ggggcgagaa gggcaaatgg aatcttgagc agcgcgatgg
    10381 caaaactggc gaagaaaccg agttgcaact gagcctgctg ggtagccagg atgagatcgc
    10441 tgaggtaggc ttcccgtact ttggtggcga cggcacggaa cacttcaaca aagtggaact
    10501 ggaaaacgtg ctgctgcaca aactgccggt gaaacgcctg caactggctg atggcagcac
    10561 cgccctggtg accaccgttt atgatctgac gctggcaaac tacggtctgg aacgtggtct
    10621 gaacgacgtt aactgtgcaa ccagctatga cgatgtgaaa gcttataccc cggcctgggc
    10681 cgagcagatt accggcgttt ctcgcagcca gattattcgc atcgcccgtg aatttgccga
    10741 taacgctgat aaaacgcacg gtcgttcgat gattatcgtc ggtgcggggc tgaatcactg
    10801 gtatcacctg gacatgaact atcgcggtct gatcaacatg ctgattttct gcggctgtgt
    10861 cggtcagagc gggggcggct gggcgcacta tgtaggtcag gaaaaactgc gtccacaaac
    10921 cggctggcag ccgctggcgt ttgcccttga ctggcagcgt ccggcgcgtc acatgaacag
    10981 cacttcttat ttctataacc actccagcca gtggcgttat gaaaccgtca cggcggaaga
    11041 gttgctgtca ccgatggcgg ataaatcccg ttataccgga cacctgatcg actttaacgt
    11101 ccgtgcggaa cgca
//
LOCUS       DAMZET010000138        11087 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.138, whole genome shotgun sequence.
ACCESSION   DAMZET010000138 DAMZET010000000
VERSION     DAMZET010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.138"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..614
                     /gene="argS"
                     /locus_tag="POB34_003630"
     CDS             <1..614
                     /gene="argS"
                     /locus_tag="POB34_003630"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDI5920505.1"
                     /translation="KDGKPFKTRAGGTVKLADLLDEALERARRLVAEKNPDMPADELE
                     KLANAVGIGAVKYADLSKNRTTDYIFDWDNMLAFEGNTAPYMQYAYTRVLSVFRKAEI
                     NEEQLAAAPVIIREDREAQLAARLLQFEETLTVVAREGTPHVMCAYLYDLAGLFSGFY
                     EHCPILSAENEEVRNSRLKLAQLTAKTLKLGLDTLGIETVERM"
     gene            791..1279
                     /locus_tag="POB34_003631"
     CDS             791..1279
                     /locus_tag="POB34_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HDI5920506.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(1399..1791)
                     /gene="flhe"
                     /locus_tag="POB34_003632"
     CDS             complement(1399..1791)
                     /gene="flhe"
                     /locus_tag="POB34_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HDI5920507.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(1791..3869)
                     /gene="flhA"
                     /locus_tag="POB34_003633"
     CDS             complement(1791..3869)
                     /gene="flhA"
                     /locus_tag="POB34_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDI5920508.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPRMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(3862..5010)
                     /gene="flhB"
                     /locus_tag="POB34_003634"
     CDS             complement(3862..5010)
                     /gene="flhB"
                     /locus_tag="POB34_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HDI5920509.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(5212..5856)
                     /gene="cheZ"
                     /locus_tag="POB34_003635"
     CDS             complement(5212..5856)
                     /gene="cheZ"
                     /locus_tag="POB34_003635"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HDI5920510.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(5867..6256)
                     /gene="cheY"
                     /locus_tag="POB34_003636"
     CDS             complement(5867..6256)
                     /gene="cheY"
                     /locus_tag="POB34_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HDI5920511.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(6271..7320)
                     /gene="cheB"
                     /locus_tag="POB34_003637"
     CDS             complement(6271..7320)
                     /gene="cheB"
                     /locus_tag="POB34_003637"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HDI5920512.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(7323..8183)
                     /gene="cheR"
                     /locus_tag="POB34_003638"
     CDS             complement(7323..8183)
                     /gene="cheR"
                     /locus_tag="POB34_003638"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDI5920513.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(8202..9803)
                     /gene="tap"
                     /locus_tag="POB34_003639"
     CDS             complement(8202..9803)
                     /gene="tap"
                     /locus_tag="POB34_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HDI5920514.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(9849..>11087)
                     /locus_tag="POB34_003640"
     CDS             complement(9849..>11087)
                     /locus_tag="POB34_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HDI5920515.1"
                     /translation="DYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIVTDNAD
                     DYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLTIDG
                     RSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASALE
                     ETAASMEQLTATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEIADSSKKI
                     ADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQAAKEIKA
                     LIEDSVSRVDTGSVLVESAGETMNNIVNAVTRVTDIMGEIASASDEQSRGIDQVALAV
                     SEMDRVTQQNASLVQESAAAAAALEEQASRLTQAVSAFRLAASPLTNKPQTPSRPASE
                     QPPAQPLLRIAEQDPNWETF"
BASE COUNT         2615 a         3161 c         2717 g         2594 t
ORIGIN      
        1 gtaaagacgg caaaccgttc aaaacccgcg cgggtggtac agtgaaactg gccgatctgc
       61 tggatgaagc cctggaacgt gcacgccgtc tggtggcaga aaagaacccg gatatgccag
      121 ctgacgagct ggaaaaactg gctaacgcgg ttggtattgg tgcggtgaaa tatgcggatc
      181 tctccaaaaa ccgcaccacg gactacatct tcgactggga caacatgctg gcgtttgagg
      241 gtaataccgc gccatacatg cagtatgctt acacgcgtgt attgtccgtg ttccgtaagg
      301 cagaaattaa cgaagagcaa ctggctgcag ctccggtaat catccgtgaa gatcgtgaag
      361 cgcaactggc agctcgcctg ctgcagtttg aagaaaccct caccgtggtt gcccgtgaag
      421 gcacgccgca tgtgatgtgt gcttacctgt acgatctggc tggtctgttc tctggcttct
      481 acgagcactg cccgatcctc agcgcagaaa acgaagaagt gcgtaacagc cgtctgaaac
      541 tggcacaact gacagcgaag acgctaaagc tgggtctgga tacgctgggt attgagacag
      601 tagagcgtat gtaatcgatt tttcgtgaga gtaaagcctg atcagggtta cctgatcagg
      661 cttttttatt gccatctaaa tgtattctga aaatggacat gccattgttt tctgactgtt
      721 ggataagagg ccagaagcgt aatatccggc cccagggaaa cgataacggt tgaatttaag
      781 gaataccgca gtgtttaaat ttcttgtatt aacattaggc attatctctt gccaggctta
      841 cgcagaagat acggttatag taaacgacca tgacatttca gccatcaaag attgttggca
      901 aaaaaattca gatgatgata ctgacattaa cgtgatcaaa tcatgcctgc gacaagaata
      961 caatctcgtc gatgcgcaat taaataaagc ctatggtgaa gcttatcgtt atatagaaca
     1021 agtgccacgc acaggtgtaa aaaaacctga taccgaacaa cttaacttgc ttaaaaaatc
     1081 acagcgagcc tggctggatt ttagggacaa agaatgtgaa ttaatccttt caaatgagga
     1141 cgttcaggat ttaagtgacc cttattctga atcagaatgg ctctcatgta tgatcataca
     1201 gaccaatacg cgaactcgcc agttgcagct ataccgtaac tctgaagatt tttatccaag
     1261 ccctttgaca agaggataat tcacatcttt ttggcatgtt ttgttgcaag ctattcctga
     1321 taaataattg caacaagaca tcgagccttt ttcactgagt tattaaacat actcgcgagc
     1381 gcgtaatttt tttgtccttc agcgataatt cacaatcact tcattacgtt gtacctttag
     1441 cggtggaatt aaccgcccac cgcctggcac ttcccagata aatcgcaacg gttctgctgc
     1501 cgctataccg gaaaaggcca cggtggttcc gctctgcccc tctaattcga cacaacgaga
     1561 ttgcgaacac aagcgaaccc gcagtcctga aggtgtcggg ccgataagct gataacgcca
     1621 cgctaccagc gtcatcaatc ctgaagcagg ttgtcgcgta gaaagaggcg cagacgacat
     1681 cgactcaccg cgatgattta gcgtaattcc tacactactt gcctgccaca tcccctcccc
     1741 ggcggcttgc accagcagcg gaaacaataa tattgctaat aaggttctca ttatttgccg
     1801 ccaattgtcg ccgtcatgcg gatatgtcgg ttatcagaca gttccagatt cgacaggacc
     1861 actaactgcg gcaagctgcg gcgcaggaag cgagacaata atggtcgcag cgcgtggttc
     1921 accaacaata ctggcggcgc acccagcatc tcctgacggg atagcgcttc ctgagtttgc
     1981 gccagtaaac gatccgccag ccctggctcc agtcctcccc cgccctgcag cgcctgtagt
     2041 aacaaacgtt ccagcggtgt atcgaggcca ataacatgga cttcatcttt gccaggaaac
     2101 cactgctggg taatcgcccg tcccaacgcc acgcgcacga cggcggttaa ttcatgtgga
     2161 tcgctttgga tgggcgcatg ttccgccagc gtttcgagaa tggtgcgcat atcgcgaatc
     2221 ggtacttttt catcgaggag attttgcagc actttatgca gtgtggtgag cgtgacgacg
     2281 ccaggaacga gatcttccgt cagctttggc atctcctggg cgacgcgatc caacagctgt
     2341 tgcgcctcct gacgaccaaa cagctctgcg gcatgctggc taatgaggtg gttaagatgc
     2401 gttgctacca ccgtgctggc ctcaaccact gtgtacccct gaatctgcgc ctgttctttt
     2461 agcgcacttt caatccagat agcattcagg ccaaatgccg gatcgacggt cgcctcacca
     2521 ggtaacgtcc cggcagcggt tccagggtta atcgccagcc agcgccccgg ataagcatca
     2581 ccactgccaa tctccacgcc tttcatcaaa atgcgatagc gggcaggttg cagatccata
     2641 ttgtcgcgaa tgtgcaccac tggcggcaga aatcccatct cctgggcaaa tttcttgcgg
     2701 atactgcgta tacggcccaa caactcacca tcctgctgga aatcgaccat cgggatcagt
     2761 cgataaccca cttccattcc cagagaatct tccagttgta catcgttcca cgtcgcttcg
     2821 acaacggtat tattctctgc catttttacc ggtttgggtt cggcaggcgc tttttgttcg
     2881 cgtccgcgta tccaccaggc cagccccagc aatccggcag tgaacagcaa aaataccagg
     2941 ttcggcattc taggcaccag gccgagtaaa ccgagcacgg cggcgcttaa caacataacg
     3001 cttgggttac tgaaaagctg attcaccatc tgctcgccaa catcctgatc ggtgctgaca
     3061 cgcgtaacga tgacccccgc ggcggtagaa atcaccagcg ccggaatttg tgccaccaga
     3121 ccgtcgccaa tggtcaatag cgtataactt tccgccgcgt gtcccatgct catgccatgt
     3181 tgcagcacgc cgaccagcaa cccgccgaca acgttaatga ccatgatgag gatcccggcg
     3241 atggcatcgc cgcgaacaaa cttacttgcc ccgtccattg agccgtaaaa atcggcttcc
     3301 tgagtcactt cggagcggcg ttttttcgcc tcatcttcac caatcaatcc agcgttaagg
     3361 tcggcgtcaa tcgccatctg cttacccggc ataccatcga gaacaaagcg cgcacccact
     3421 tctgcgatac gcccggcacc tttggtaatg accataaagt tgatgatcac gagaatgaca
     3481 aacaccacga tacctatagc gaaattgcca ccaacgagga agtgaccgaa cgcttcgacc
     3541 accttccctg ccgccgccgc gccggtatgc ccttccatca aaatgatacg ggttgaagcc
     3601 acgttaagtg ccagacgcaa caacgtagta aacaacagaa tggtcggaaa cgcagcaaac
     3661 tcaagcgtgc gctgggtaaa catcgccacc agcaacacca tgatcgacaa ggcaatattg
     3721 aaggtaaaca acaggtcgag tatgaatgcg ggcagtggca gcaccatcat cgacaagatc
     3781 aacaggatca aaatcggtcc ggcaaggatc tgccattgtg tcgatttcag gtttgcgggc
     3841 aggcgcagca tcgcggccag attactcatg ggtcggtttc tcgttaataa aatccagggc
     3901 ttccggcacc ggaagatgag taggttgtac agggcgctgt ccaccagcca gacgccagcg
     3961 tttcagttgc cagacccagg ccagcacttc cgccaccgcg gcgtacagtt gacccgggat
     4021 ttgttgacca atctccgcat gtcgatacag cgctcgcgcc agcggcggcg cttcaagcgt
     4081 cgggacgtta ttttcagcgc caatttcacg aatgcgcagc gcgaccagcc ctgcaccttt
     4141 agcgaccact ttcggtgcgc tcattttgtt ttcgtcatac tgcaacgcta ccgaatagtg
     4201 ggtcgggtta ttgacaatga catccgcttt cggcacatcg gccatcatcc gacgccgtgc
     4261 agcagctcgc tgcatctgac ggatccgccc tttaacatga gggtcacctt cgctttgttt
     4321 gaactcatca cgaatatcct gccgtgacat acgcagcttt ttcaggtggc tgaatatttg
     4381 gaaaaagacg tcaaatccca ccattggaat gacaccaagc accaccagca gtgcgcatag
     4441 ccctaccaaa tccatcgcat tacccatggc ggtaatcgga gactcggcca tcaagcgcat
     4501 catctgcggc cagtgatgcc agagaaaaaa ccccgtcacg ctgccaacca ggatggtttt
     4561 caaaattgct ttaagcaact ccgcgccagt ctgagccgag aacatccgtt taatgcccgg
     4621 tagcgggttg agtttggaaa acttcggctg caaggatttg ccgctaaata ccagccctcc
     4681 cagcatgacc ggagaaatga gcgccaccag caccacgccg ctaatcagcg gcagcagcgc
     4741 cagcatggct tctctgatca gcagaataat ctgcccgagg atcagattcg ggtcattgat
     4801 aatactgtga tcaaaatgca gcccagcgga gagcatgccc gacaatcgac gggccagcga
     4861 cacaccgcca aaccagataa cactaacgcc caccagcaaa atcagcagtg aggtcagttc
     4921 acgggaacgc gggatttgcc cctcttcccg cgctttttct agtcggtgag gtgtgggggc
     4981 ttctgttttg tcgtcgctct cgtcagacac gtcgccaatc ctggaaagag tattaagcgt
     5041 gaatgatgcc agagcgcaaa gcgttcaatg gtttgagtaa ggggcaaaac aggcgggatt
     5101 tagggctttt gctgccacac atcaagcata gtgtgcgttt gtcggatgcg gcgtcatcgc
     5161 cttatcagac cgcctgatat aacgtggtca cgccacatca ggcaatacaa atcaaaatcc
     5221 aagactatcc aacaaatcgt ccacctgatc ctgactggct accacaccgg ctttgctggt
     5281 atcgacctga ggtccattaa gcaaactctg gttttcacgt tttggacgcg actcctgttc
     5341 cgggatgttt tccaacagca ccatcagcaa ctggcgttcg atctcctgaa tgacatccat
     5401 catccgctta ataacctgtc cggtgagatc ctgaaaatcc tgcgccatca tgatttccag
     5461 cagttgcgcg tttgtaaagc tggtatgcgc gggtacatct gccagaaatt gccgcgtgtc
     5521 tgttaccagt tcacgggcgt cggcaaggtc aatcggatcg gcaaaccagt catcccaacg
     5581 ttgggttaac gcttttgctg atttctccat ttgatcctga tgcggttgtg acgcctcaac
     5641 actgttcagc gcccgctccg cagcctgggc ggtcatctgc acaacatagt acaaacgatc
     5701 gcgcgcatcg gggatggctt ccgccgcttc ggcaatggcc tgatccagcc ccagttcccg
     5761 caaactgtcg cgcagcatac gcgtcaggct gccgatgcgc gcaatgatat cgccagctga
     5821 atgctcgtca gcaggtttga ttgatggttg catcatagtc gcatcctcac atgcccagtt
     5881 tctcaaagat tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggc ttcaccacat
     5941 agccactggc ccccgcttgc gccgcagcaa tgatgttctc tttcttcgct tctgcagtca
     6001 ccattaacac tggcaatgcc gacatcgcgc catccgcacg aattgttttc agcaattcca
     6061 ggccatccat attgggcatg ttccagtcgg agataacaaa tccataaccg cctgcctgca
     6121 acttattgag agcgtcgacg ccatcttccg cttcctcaac attattgaat cccagctctt
     6181 tcagcaggtt acgcactatg cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa
     6241 gttctttatc cgccatttca cactcctgat ttaaatacgt atcgcctgtc cggcactaat
     6301 ttttgccaac atttgctggc ttacctggct aagatcgacc acttcgcaga caccacccat
     6361 attgatggcc tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt gcgcaagggt
     6421 ccatgccccc gcctgacgca tcgccaacat tcctgccgcg ccgtcgttgc ccataccggt
     6481 caggatcacc ccaaccgcat tacgccccgc ctgtttggcg acagaatgga acaacacatc
     6541 taccgaaggc cgatgacggt taaccgccgg gccatcatga attttgattt ggtaatttgc
     6601 gccactacgc gccagctcca tatgccgatc gcccggcgcg atataggcat gccccggcaa
     6661 aacacgttct ccgtcttcgg cttctttaac cccgatctga caaagcttat taagtctgtc
     6721 ggcaaaagag cgggtgaaac caggcggcat atgctgggta attaacagtg ccgggctgga
     6781 aagcggcaac ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat
     6841 cgcaatcagt ttttcagaac tcaacaacgg ccctgccttc agcgttgtcg gtgccgacaa
     6901 tggcttatgt gctgcaaggc tcgcctttgc tgctgtacgc accttttcag caatcatttc
     6961 gctatacgcc agcattcctt cgcgaatacc cagttgcggt ttggtgacaa aatctatcgc
     7021 ccccagctcc agcgcgcgca gcgtgacttc tgaccctttg ccggtcaggg aagaaaccat
     7081 cactacgggc attggacgca aacgcattaa tttttcgagg aaatccagtc cgtccatccg
     7141 cggcatttca acatccagcg tcagcacatc gggattgaat ttcttaatca agtcacgcgc
     7201 gaccagcgga tcaggcgcgg tcgccaccat ttccatgtcg ctatggctgt tgatgatttc
     7261 tgtcatgatc tggcgcatca gtgccgaatc atcgacagat aacaccctga ttttgctcat
     7321 cgttaatcct tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg
     7381 tggctaaagt tttcagagtg acccgcaaac agcaatccgt cgggtttaag gagcggaaca
     7441 aagcggcgca aaatctcctg ctgggtattt tgatcgaagt agatcatgac gttacgacag
     7501 aagatcgcat caaacggccc cggcacggtg tactgtttcg ccagtagatt cagcggggca
     7561 aaatcaacat agttcgccag ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc
     7621 cctcgcatga aataccgttg cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga
     7681 taaataccgc ttctggcttt ttccagcact tcggtgtcga tatcactggc aaacactttc
     7741 cagcgtccgg gcgcggtgcc caatgtgtca gccagcgtca tcgcaatgct gtacggctct
     7801 tcgccggtcg aagccgccgc gctccatacg cgatactcgc cagaacggcg acgtgcgtga
     7861 tccgcgagca gagggaaatg gtgtgcctca cggaaaaatg ccgtcagatt cgtggtcagc
     7921 gaattgataa acgcctgcca ctcaccgctg tgctgattag attccagcaa gttcagataa
     7981 tgaccgaaat ccgtcagtcc cagcgaacgc aaacgacgaa ccagtcggtt gtaaaccatg
     8041 tcgcgtttat ggtcagccag aacgatcccg gctcgttgat agatcaattg acttatccgc
     8101 cgaaaatgcg cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa agacgtttgc
     8161 ccacagggca gagatgaagt catagcgcct tctcaatcac ttcaggatac cactggcgca
     8221 atttgtaact gcgccgactc atgtcgtgct acttcatgtt cttcaagggt aaataccgcc
     8281 acacgcgacg aaagatggtc ggcctgattc gccagttgtt ccgttgccac cgccgcttct
     8341 tctaccagcg aggcgttctg ctgagtcacc tgatccatct ggctgacagc ctgtgcaacc
     8401 tgctcaatcc cccgctgttg ttcttccgac gcagaggcta tttctcccat aatgtcgttc
     8461 acgcgggtca ccgaactgac aatatcgatc atggtcgcgg cggcgttatt caccagtttc
     8521 gaaccctgct ggacacgatt gactgactct tcgatcagcc ctttgatctc ttttgctgcc
     8581 tgcgcgctac ggctggcaag attgcgcact tcacctgcca ctaccgcaaa accacgcccc
     8641 tgctctccgg cgcgagccgc ttccactgcc gcattcaggg ccagaatatt ggtctggaaa
     8701 gcaattccgt cgataacgct gataatgtcg ccaatttttt gcgagctggt ggcgatctcc
     8761 tgcatggtgt gagtcatggt actgacctga acaccgcccg cctgcgccgt tgtcgcggca
     8821 ttttttgcca gttccgacgc ctgtcgtgcg ttatcggcgt tttgccctac cgtggcggtt
     8881 aattgctcca tactggcggc cgtttgtgcc agcgatgccg cctgctgttc ggtacgactt
     8941 gagagatcgt tattgcctgc gacaatctcc gcgataccaa tgtgcatctc ctggcttccc
     9001 ttacgcacat cacttaccgt cccacgcaaa gcctgctgca tggtcttcag actggcaaaa
     9061 atggcggtga tctcattacg accatatacc gcaatcggac gcgccagatt acccgcagca
     9121 atgctgtcaa aatggctacc gataatggcc agtggttgaa caatcatctt gcgcgtccac
     9181 cacagcgcac tgctgatgta gatcgccgca acaataatca tgctgataaa caccagtgcc
     9241 gaaatctgat agttacgctg gctttgcgca ctggcggctt ccagcacatg gttgatctcc
     9301 agctgccagg cctcaaagtt aacgtcaaac gcattctgtg atccctgcac cggcgcagtg
     9361 aggaaatccg aaagttgatt actttcaagc caggtcgcct gatgttcgag atcgttgtgc
     9421 cagcgggcaa aacttttctc cgtctctttt tgcaatgccc tgacgtgctc gttgcccgca
     9481 gtcatcgcca taaaactttt aaacagcgtg gtggattgcg tcagactggc gcgcgccgtc
     9541 gtcatcaacg ttttaatgtc atccgccgga tagctaagtg ccgtcagagt gcccgctttg
     9601 ttcagcgcgg tactggcctg taacattacc gcccgagttt gcgctaatgc cgcacgttgc
     9661 tgattgctct gctcgacctg attcagtcgt tgcaaatcgt cgcgaaatgc ccaaaaagac
     9721 atgccgttac tgccaatctg caagatcccg cagagaatca aaattaaaaa cagcgtggtc
     9781 gaaattcgaa tacgattaaa catcaacgct ccccatcagg cggcaatgac cgcgttagta
     9841 aatactcgtc aaaatgtttc ccagtttgga tcttgttcag caattcgcag tagtggctgt
     9901 gccggtggtt gctcactggc aggacgggat ggtgtttgcg gtttattggt gagtgggctg
     9961 gctgccagac ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc ttccagcgca
    10021 gcggcggcgg cagctgattc ctgcaccagc gatgcgttct gttgcgtgac gcgatccatt
    10081 tccgaaaccg ccaatgcgac ttgatcgatg ccacggctct gttcatccga tgccgatgca
    10141 atctcgccca taatgtcagt cacgcgagtg acagcattga cgatattgtt cattgtttcc
    10201 ccggcgcttt cgaccagcac cgaaccggta tcaacgcgtg agacggagtc ttcaatgagg
    10261 gctttgatct cttttgccgc ctgggcgctg cgactggcaa gattacgcac ttcacccgcc
    10321 accacggcaa aaccacggcc ctgttcaccc gcacgcgcgg cttcgaccgc ggcattcagc
    10381 gcgaggatat tagtctggaa ggcaataccg tcgataacgc tgataatgtc ggcaattttc
    10441 ttcgaactat cggcgatctc atgcatcgtt ttcactacgc catccaccac tttgccgccg
    10501 tgctgggcgg tgtcggaggc actttgcgcc agttgcgagg cctggcgggc gttatcggcg
    10561 ttttgcttca ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc cagcgcggat
    10621 gcctgctgtt cagtacggga ggaaagatcg gtgttgcccg ccgcaatttc acgggtaccg
    10681 gcatagatgg catctgaacc ttcgcggaca tgagtgacgg tgtcagtcaa agagcgttgc
    10741 atatgtgaaa cgctctgcgc caggtcgccc atttcactgc gcccgtcaat ggtcagggta
    10801 ttcgccaggt taccaccggc gatttcgcga atgtgagcaa taatttttgc cagcggagta
    10861 agcaacatac ggcgaatgcc gtaccacgcc accagcagaa tcaataccac caccagcgcg
    10921 ataaccgcca gttgccactg ggcaaatcgg taatcatctg cgttgtcagt gacgatatcg
    10981 cgatacagtt tttcactgct gagggcgtac tgagcaaacg cttcgcccat tgcattttgc
    11041 attccctggg ttggctgagc gaaataagct ccagtattgc cataatc
//
LOCUS       DAMZET010000139        11070 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.139, whole genome shotgun sequence.
ACCESSION   DAMZET010000139 DAMZET010000000
VERSION     DAMZET010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.139"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..749
                     /gene="pfkB"
                     /locus_tag="POB34_003641"
     CDS             <1..749
                     /gene="pfkB"
                     /locus_tag="POB34_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HDI5920516.1"
                     /translation="GGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGEQYR
                     FVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQGIRC
                     IIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKAKRV
                     VVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMVRFG
                     VAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            850..1140
                     /gene="ghoS"
                     /locus_tag="POB34_003642"
     CDS             850..1140
                     /gene="ghoS"
                     /locus_tag="POB34_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDI5920517.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            1246..2106
                     /locus_tag="POB34_003643"
     CDS             1246..2106
                     /locus_tag="POB34_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDI5920518.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSSNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(2147..2683)
                     /locus_tag="POB34_003644"
     CDS             complement(2147..2683)
                     /locus_tag="POB34_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HDI5920519.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            2830..3498
                     /gene="hxpB"
                     /locus_tag="POB34_003645"
     CDS             2830..3498
                     /gene="hxpB"
                     /locus_tag="POB34_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HDI5920520.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3661..4251
                     /locus_tag="POB34_003646"
     CDS             3661..4251
                     /locus_tag="POB34_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDI5920521.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            4384..5775
                     /gene="tcyP"
                     /locus_tag="POB34_003647"
     CDS             4384..5775
                     /gene="tcyP"
                     /locus_tag="POB34_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HDI5920522.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFATFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(5779..6594)
                     /locus_tag="POB34_003648"
     CDS             complement(5779..6594)
                     /locus_tag="POB34_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920523.1"
                     /translation="MRWEMFSTNLIQSNYGDLNIKTLAFDSFKERLESTMAALTFFIS
                     TGQCDCDEIAESNFNRMIAYMSNINYDASKPGAPALSFDTYLQDNVKYRVIINNLYGS
                     EIRIRGINKDFIGMDVTSVFRPEKMTNLINIKNRLVIHYLRTIYYEENYIHPAGSIFA
                     AIQKNESLLKFPSIISMLNINLLFNPLNLPGMGSGVLEDIMSIPDSSLRKRLGYEVLS
                     FSLQAHSLSQECIDKLDIFFAGDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
     gene            complement(6871..7134)
                     /gene="cedA"
                     /locus_tag="POB34_003649"
     CDS             complement(6871..7134)
                     /gene="cedA"
                     /locus_tag="POB34_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HDI5920524.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            7317..9578
                     /gene="katE"
                     /locus_tag="POB34_003650"
     CDS             7317..9578
                     /gene="katE"
                     /locus_tag="POB34_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HDI5920525.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(9625..10383)
                     /gene="chbG"
                     /locus_tag="POB34_003651"
     CDS             complement(9625..10383)
                     /gene="chbG"
                     /locus_tag="POB34_003651"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDI5920526.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVSRFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSHGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNLIRKSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(10396..>11070)
                     /gene="celF"
                     /locus_tag="POB34_003652"
                     /gene_synonym="chbF"
     CDS             complement(10396..>11070)
                     /gene="celF"
                     /locus_tag="POB34_003652"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDI5920527.1"
                     /translation="SGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLA
                     IEMGEYYKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAI
                     YNDKQAEHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTI
                     KGFEIAASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIA
                     ELKKAH"
BASE COUNT         2851 a         2752 c         2631 g         2836 t
ORIGIN      
        1 cgggtggcgc gaccggcgaa cacctggttt ctctgttggc ggatgaaaat gtccccgtcg
       61 ctactgtaga agccaaagac tggacccggc agaatttaca cgtacatgtg gaagcaagcg
      121 gtgagcagta tcgttttgtt atgccaggcg cggcattaaa tgaagatgag tttcgccagc
      181 ttgaagagca agttctggaa attgaatccg gggccatcct ggtcataagc ggaagcctgc
      241 cgccaggtgt gaagctggaa aaattaaccc aactgatttc cgctgcgcaa aaccaaggga
      301 tccgctgcat catcgacagt tctggcgaag cgttaagtgc agcactggcg attggtaaca
      361 tcgagttggt taagcctaac caaaaagaac tcagtgcgct ggtgaatcgc gaactcaccc
      421 agccagacga tgtccgcaaa gccgcgcagg aaatcgttaa tagcggcaag gccaaacggg
      481 ttgtcgtttc ccttggtcca caaggagcgc tgggtgttga tagtgaaaac tgcattcagg
      541 tggtgccacc accggtgaaa agccagagta ccgttggcgc tggtgacagc atggtcggcg
      601 cgatgacact gaaactggca gaaaatgcct ctcttgaaga gatggtccgt tttggcgtag
      661 ctgcggggag tgcagccaca ctcaatcagg gaacacgtct gtgctcccat gacgatacgc
      721 aaaaaattta cgcttacctt tcccgctaac aaaaacattc ccccagcatt gggggaatca
      781 ccaccaacct gtcggcaacg cgtttctccg actatgctca aaagtcatgt gataacaaag
      841 gggtgaacta tggccagtgg cgatcttgtc cgttatgtca taaccgtcat gttgcatgag
      901 gatacattga ctgaaattaa cgagttgaat aattacctga ctcgcgacgg ttttttgctc
      961 accatgacgg atgatgaggg aaatatccat gagctgggga ctaacacttt tggacttatc
     1021 agtacccaaa gtgaagaaga aattagagaa ctggtttcgg ggcttaccca aagtgcaacc
     1081 ggcaaagatc ctgaaatcac catcacgacc tgggaggaat ggaatagcaa cagaaaataa
     1141 atggtttttg ggcaataatc agtctgtgat gtgcgttagc tcgtgttttt acaccgcatt
     1201 cttgcgctaa ccttatgatc tggcagacaa catgggagag acatcatgtg gcaggcaatc
     1261 agtcgtcttt tgagcgagca gttaggtgaa ggcgaaatcg aactgcgtaa tgaactgcct
     1321 ggcggagaag tccatgccgc atggcatttg cgctatgcag gacatgactt tttcgtcaaa
     1381 tgtgatgaaa gggaactgct tcccggtttt accgccgaag ccgaccaact ggagttactg
     1441 tcgcgtagta aaaccgtcac cgtgcctaag gtttgggcag taggcgctga ccgtgactac
     1501 agttttctgg tgatggatta tctcccacct cgtccgctgg atgcgcatag cgcatttatt
     1561 cttggtcagc aaattgcgcg tttacatcaa tggagtgacc aaccacaatt tggcctcgat
     1621 tttgataact cgctctccac aactccacag cccaacacct ggcaacgtcg ctggtcaacg
     1681 ttttttgctg aacaacggat tggctggcag ttggaactgg cagcagagaa agggatcgct
     1741 ttcggcaata tcgacgccat cgtcgagcat attcagcagc gtctggcctc gcatcaaccg
     1801 cagccttctc tgttgcacgg cgatttatgg tccagcaact gtgcgctggg tccggatggc
     1861 ccgtacattt tcgaccctgc ctgctactgg ggtgaccgag agtgcgacct ggcgatgtta
     1921 ccgctgcata ctgaacagcc gccacaaatc tatgacggct atcagtcagt atccccgcta
     1981 cctgccgatt tccttgaacg tcaaccggtt taccaactct acacgctgtt aaatcgtgca
     2041 agattatttg gcggtcagca tttggttatt gctcagcagt cattggatag attattagca
     2101 gcatgatatg ggttgaggat aatggccgct ccgtgcggcc ttttgattaa ataaacccaa
     2161 gcagagaaaa gaacacataa cctgcagcaa tacaaataac cggcaggata tacagtgaga
     2221 aaaactgcag gaaaatagta tggtgcggaa caacaatacg ggactcaatt tgttcacgcg
     2281 ttagcccctc ttcccctttg gctttttcca gaatgagttg atcttcaaca ccttcccgca
     2341 agaagcgcgc ctggcgactc atccgggcac cggaatcctg caacgccagc ccgacaaaaa
     2401 tcaggatgaa aatcacccag aacataacgt tcacgccacc attaaaatta ggggtcgggg
     2461 agttatacca gaacagattc aaaaaaggcg tattcgcctg catcatatcg atcatgacat
     2521 gagtaaagtc gagcatgacc gcattaatgc cttcctggtt ttcctgccgg gtattcataa
     2581 atttcagcaa agaaatcaat gtagagatca atgcaggtat aaaaatcacc caacccaaaa
     2641 tccttttcaa aacagcaatg cgtccagctt gttgatacgt catgagttct ccttgattaa
     2701 gacgcgtcgt ttcacttagt ttacctgtag atatctgttt tcgcccattc tttaaaggcg
     2761 atatgatagg cgcttaatca taagcacggc ttaatacctt acacataatg ctctaaagga
     2821 gaggttgtaa tgtcaacccc gcgtcagatt cttgctgcaa tttttgatat ggatggatta
     2881 cttatcgact cagaaccttt atgggatcga gccgaactgg atgtgatggc aagcctgggg
     2941 gtggatatct cccgtcgtaa cgagctgccg gacaccttag gtttacgcat cgatatggtg
     3001 gtcgatcttt ggtacgcccg gcaaccgtgg aatgggccaa gccgtcagga agtagtagaa
     3061 cgggttattg cccgtgccat ttcactggtt gaagagacac gtccattatt accaggcgtg
     3121 cgcgaagctg ttgcgttatg caaagaacaa ggtttattgg tgggactggc ctccgcgtca
     3181 ccactacata tgctggaaaa agtgttgacc atgtttgact tacgcgacag tttcgatgcc
     3241 ctcgcctcgg ctgaaaaact gccttacagc aagccgcatc cgcaagtata tctcgactgc
     3301 gcagcaaaac tgggcgttga cccactgacc tgcgtggcgc tggaagactc ggtaaatggc
     3361 atgatcgctt ctaaagcagc ccgcatgcgt tccatcgtcg ttcctgcgcc agaagcgcaa
     3421 aatgatccac gttttgtatt agcaaacgtc aaactctcat cgctgacaga actcaccgca
     3481 aaagaccttc tcggttaatg accaggggca gtgatcacgc tgcccctggt tctttatctg
     3541 aatttgcatt caactgacgg attaatcgtc aatttaagag aaagagttac accgtcacca
     3601 cttccgtgca ctgtataaaa atcctatact gtacgtatcg acagtttagt gagttttatc
     3661 atgacggcgg aaggtcacct tctcttttct attgcttgtg cggtatttgc caaaaatgcc
     3721 gagctgacgc ccgtgctggc acagggtgac tggtggcata ttgtcccttc cgcaatcctg
     3781 acgtgtttgt taccggacat cgatcaccca aagtcgtttc ttgggcagcg attaaaatgg
     3841 atatcaaaac cgatcgcccg cgcttttggg catcgtggtt ttacccacag tctgctggcg
     3901 gtatttgcgc tgctggcaac cttttacctt aaggttccgg aaggctggtt cattccggct
     3961 gatgcgctac aaggaatggt gctgggttat ttgagccaca tacttgccga tatgctgaca
     4021 cccgccggtg ttcccctgct ctggccatgc cgctggcgtt tccgcttgcc tatcctggtt
     4081 ccccaaaagg gcaaccaact ggaacgtttt atctgcatgg cattatttgt ctggtcggta
     4141 tggatgcccc attcattacc cgagaacagc gctgttcgtt ggtcatcgca aatgatcaat
     4201 accttgcaga tccagtttca tcggcttatt aagcatcagg ttgaatacta aaaaggcaaa
     4261 aatcaccttt ctggaataag caattccatt tgaatataag agccagctca cagttctgtt
     4321 aatcttgcgc caacactatg actgctacgc agtgatagaa ataataagat caggagaacg
     4381 gggatgaact ttccattaat tgcgaacatc gtggtgttcg ttgtactgct gtttgcgctg
     4441 gctcagaccc gccataaaca gtggagtctg gcgaaaaaag tgctggtggg tctggtgatg
     4501 ggtgtggttt ttggccttgc cctgcatacc atttatggtt ctgacagcca ggtacttaaa
     4561 gattctgtac agtggtttaa catcgttggt aacggctatg ttcaactgct gcaaatgatc
     4621 gttatgccgt tagtcttcgc ctctattctg agcgcggttg cccgtctgca taacgcatct
     4681 cagttaggca aaatcagttt tctgaccatc ggtacgcttt tgtttaccac gctgattgcg
     4741 gcgctggtcg gtgtgctggt caccaacctg tttggtttga cggctgaagg tctggttcag
     4801 ggtggtgcag aaactgcacg tctgaacgcc attgaaagta actatgttgg taaagtctct
     4861 gatctgagcg ttccgcagct ggtcttgtcc tttatcccga aaaacccgtt tgccgatctt
     4921 accggagcca atccgacgtc aattatcagc gtggtaattt ttgccacatt cctcggcgta
     4981 gctgcattga aactgctgaa ggatgatgcg ccgaaaggtg aacgcgtctt agccgctatc
     5041 gataccctac aaagctgggt gatgaaactg gttcgccttg tcatgcagtt gaccccttac
     5101 ggcgttctgg ctctaatgac caaagtggtt gcaggttcta acttgcaaga catcatcaaa
     5161 ctgggaagtt tcgttgtcgc gtcctacctc ggtctgctga ttatgtttgc agtgcatggc
     5221 attctgctgg gcattaatgg cgtgagtccg ctgaagtact tccgtaaggt atggcctgtg
     5281 ctgacgtttg cctttaccag ccgttccagt gctgcgtcta tcccactgaa tgtggaagca
     5341 caaacgcgtc gtctgggcgt tcctgaatcc atcgccagtt tcgccgcctc tttcggtgca
     5401 accattggtc agaacggctg cgccggtttg tatccggcaa tgctggcggt gatggttgcg
     5461 cctacggttg gcattaaccc gctggacccg atgtggattg cgacgctggt cggtattgtt
     5521 accgttagtt ccgcaggcgt tgccggtgtc ggtggtggtg caactttcgc cgcactgatt
     5581 gtgctgcctg cgatgggcct gccagtaacc ctggtggcgc tgttaatctc cgttgaaccg
     5641 cttatcgaca tgggccgtac ggcgctaaac gttagtggct cgatgacagc tggcacgctg
     5701 accagccagt ggctgaagca aaccgataaa gccattctgg atagcgaaga cgacgccgaa
     5761 ctggcacacc gttaattctt atgctggcaa cggcccgttt tgtatagggg ccgttgcctt
     5821 actttttaaa tgttccatcg cggcgatgca tactgactca tatttaaaca gatcgccagc
     5881 gaaaaaaatg tccaacttat caatacattc ctggctcaat gaatgtgcct gtagcgagaa
     5941 gcttaaaacc tcatacccga gacgttttct aagtgaacta tctggaatac tcataatatc
     6001 ttcaagcaca ccacttccca ttcccggaag atttagtgga ttaaaaagaa gattgatatt
     6061 aagcattgat attatcgaag gaaactttag gagactctca tttttttgaa tcgcagcaaa
     6121 aatgctcccg gcaggatgta tataattttc ttcatagtat atagtcctta aatagtgaat
     6181 aaccaacctg ttttttatat tgatcaaatt ggtcattttt tctgggcgaa atacagaagt
     6241 aacatccatt ccaataaaat ctttatttat acctcttatc cgaatttcac taccataaag
     6301 attattaatt attactctat atttaacatt gtcctgcaga tatgtatcaa aactaagtgc
     6361 tggcgctcct ggctttgagg catcatagtt aatgttagac atataggcaa tcatacgatt
     6421 gaagtttgat tctgctattt catcgcagtc acattgaccc gttgatataa agaaagtgag
     6481 tgccgccatg gtagactcaa gcctttcttt aaaagagtca aaagccagag ttttaatatt
     6541 taaatcacca taattactct gtattaagtt tgtagaaaac atctcccacc tcatattgtt
     6601 aacaaaatta ttatctcatt taaatctaag tcatttacaa tataagttta agcgcgacgc
     6661 cacaggatga actatcaaaa atagctcatc atgattagca aaacttaacc attttaaaat
     6721 aaaaaaacaa ttaaagaaaa aagatcactt atttatagca atagatcgtc aaaggcagct
     6781 ttttgttact ggtggtttga atgaatgtag caatgaaata cagaatttca ggtcatgtaa
     6841 ctcccggcaa aaccgggagg tacgtaatct ttactcagtc acttcccctt cctggcggat
     6901 ctgatttgcc caacgctggg cggattcagg cacagtaaac gccggtgagc gcagaaatga
     6961 ctctcccatc agtacgaacg caacatattt gccacgcagc atccagacat cacgaaacga
     7021 atccatcttt atcgcatgtt ctggcggcgc gggttccgtg cgtgggacat agctaataat
     7081 ctggcggttt tgctggcgga gcggtttctt cattactggc ttcactaaac gcatattaaa
     7141 aatcagaaaa actgtagttt agccgattta gcccctgtac gtcccacttt gcgtgtattt
     7201 cataacaccg tttccagaat agtctccgaa gcgggatctg gcttatggtc tatagttaga
     7261 gggttttttg accaaaacag cggccctttc agtaataaat taaggagacg agttcaatgt
     7321 cgcaacataa cgaaaagaac ccacatcagc accagtcacc actacacgat tccagcgaag
     7381 cgaaaccggg gatggactca ctggcacctg aggacggatc tcatcgtcca gcggctgaac
     7441 caacaccgcc aggtgcacaa cctaccgccc cagggagcct gaaagcccct gatacgcgta
     7501 acgaaaaact taattctctg gaagacgtac gcaaaggcag tgaaaattat gcgctgacca
     7561 ctaatcaggg cgtgcgcatc gccgatgatc aaaactcact gcgtgccggt agccgtggtc
     7621 caacgctgct ggaagatttt attctgcgcg agaaaatcac ccactttgac catgagcgca
     7681 tcccggaacg tattgttcat gcacgcggat cagccgctca cggttatttc cagccatata
     7741 aaagcttaag cgatatcacc aaagcggatt tcctctcaga tccgaacaaa atcaccccag
     7801 tatttgtacg tttctctacc gttcagggtg gtgctggctc tgccgatacc gtgcgtgata
     7861 tccgtggttt tgccaccaag ttctatactg aagagggtat ttttgacctc gttggcaata
     7921 acacgccaat cttctttatc caggatgcgc ataaatttcc cgattttgtt catgcggtaa
     7981 aaccagaacc gcactgggca attccacaag ggcaaagcgc ccacgatact ttctgggatt
     8041 atgtttctct gcaacctgaa actctgcaca acgtgatgtg ggcgatgtcg gatcgcggga
     8101 tcccgcgcag ttaccgcacc atggaaggct tcggtattca caccttccgc ctgattaatg
     8161 ccgaagggaa ggcaacgttt gtacgtttcc actggaaacc actggcaggt aaagcctcac
     8221 tcgtttggga tgaagcacaa aaactcaccg gacgtgaccc ggacttccac cgccgcgagt
     8281 tgtgggaagc cattgaagca ggcgattttc cggaatacga actgggcttc cagttgattc
     8341 ctgaagaaga tgaattcaag ttcgacttcg atcttctcga tccaaccaaa ctgatcccgg
     8401 aagaactggt gcccgttcag cgtgtcggca aaatggtgct caatcgcaat ccggataact
     8461 tctttgccga aaacgaacaa gcagcattcc atccaggtca tattgttcct ggtctggact
     8521 tcaccaacga tccgctgttg cagggacgtt tgttctccta taccgataca caaatcagtc
     8581 gtcttggtgg gccgaatttc catgagattc cgattaaccg tccgacctgc ccttaccaca
     8641 acttccagcg tgatggcatg catcgtatgg ggatcgacac taacccggcg aattacgaac
     8701 cgaactcgat caacgataac tggccgcgcg aaacaccgcc gggaccgaaa cgcggcggtt
     8761 ttgaatcata tcaggaacgc gtggaaggaa ataaagttcg tgagcgcagt ccatcgtttg
     8821 gcgaatatta ttcccatccg cgtctgttct ggctaagtca gacgccattc gagcagcgcc
     8881 atattgtcga tggtttcagt tttgagttaa gcaaagtcgt tcgtccgtat attcgtgagc
     8941 gcgttgttga ccagctggcg catattgatc tcactctggc ccaggcggtg gcgaaaaatc
     9001 tcggtatcga actgactgac gaccagctga atatcacccc acctccggac gtcaacggtc
     9061 tgaaaaagga tccatcctta agtttgtacg ccattcctga cggtgatgtg aaaggtcgcg
     9121 tggtagcgat tttacttaat gatgaagtga gatcggcaga ccttctggcc attctcaagg
     9181 ccctgaaggc caaaggcgtt catgccaaac tgctctactc ccgaatgggt gaagtgactg
     9241 cggatgacgg tacggtgttg cctatagccg ctacctttgc cggtgcacct tcgctgacgg
     9301 tcgatgcggt cattgtccct tgcggcaata tcgcggatat cgctgacaac ggcgatgcca
     9361 actactacct gatggaagcc tacaaacacc ttaaaccgat tgcgctggcg ggagacgcgc
     9421 gcaagtttaa agcaacaatc aaggtcgctg accagggtga agaagggatt gtggaagctg
     9481 acagcgccga cggtagtttt atggatgaac tgttaacgct gatggcagca caccgcgtgt
     9541 ggtcacgcat tcctaagatt gacaaaattc cggcgtaaat atggataagg tcacgtcccc
     9601 tggggagtga ccttaccacc ttacttacac atcaagataa ctacccaacc gataaccacg
     9661 ctcagcaatt gcatatttca acgacgctga tgtcagcact tccagctccg ttaaccgtgg
     9721 gaaacagtaa gcactcttgc gaatcaaatt atcgacaaac gccggatggc acatgacttc
     9781 cagcgagcgt tccccgcgat ggctggaatc atcaagcact tgcaggaaca gtgcctcgct
     9841 aatctcttca ccataaaacg cgctactgaa tccgtgcgaa ctgcgcagat tagccggtaa
     9901 atcaccagcg ttagatagcg gctgacggtc gatgcgcaac gcaatcccct cttcagccgc
     9961 aaacctggaa acaataggga aaatctgcgg gaacatatgc acatgatgat ggctatcaag
    10021 atgcgtaggt ttgcgtccaa atagctcaat gaaacgcaaa tactgactgg caagctcctg
    10081 agtaatttct tccagcggta aagcatcttc ttctgccaac tgccagatcc attttcccag
    10141 cacaccatcg cgggttaacc ccggcatagc tgtcagtggc ttgcccatag taaggacaaa
    10201 gtgcatccct atggccagac tcggttcatc acgactcaac tgcaccgcat ggtcaatagc
    10261 ctgcccattc accagcgccg tcgtcgatgt gacaatccca ttgcgacagg cctcgataat
    10321 gccgtagttc tgacctttgc ttaagccaaa atcatcggca ttaacaatca gtaagcgttc
    10381 cataatcagc ctcggttaat gtgctttttt aagctctgcg atgcagtcgg caaaatttgg
    10441 cagccatttc tcgtgcgcca gaatcatctc gcgtgccagc agctcagcat cgcgatcgga
    10501 atgcaccaac ggactaaggt ttagcgccag taacacatcg ttaaactctc cgctaagcgc
    10561 ggcgttgctg gcagcaatct cgaagccttt aatggtgtga atcagcccca tcactttatc
    10621 atcgaaatgc gtaatgcgcg gatgtggcgt cgcgccatcg cgccccagcg tacaggtcat
    10681 ttctaccgcc cagtctgccg gaatattatc aatatgccca tgatgcggga tattaacgta
    10741 atgttctgct tgcttgtcgt tgtagatagc gttgatcact tcgcacgctg catcagagta
    10801 ataagcccca ccgcgctgtt ccagttcttt cggcttaact ttcaactccg ggtttttata
    10861 cagctcaaaa agttgtttct cgactttctg tactacctgc gctcgtgcgc cgcctttgta
    10921 gtattcgccc atttcaatag ccagcatctc tttttgcttg aagtaataaa gcaaataaga
    10981 acacggcaac agattcagag aacgaattaa gccttcgcta aatggcagat cgaaaatatt
    11041 tttaacgcca gatgctttta actgcccgga
//
LOCUS       DAMZET010000140        10896 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.140, whole genome shotgun sequence.
ACCESSION   DAMZET010000140 DAMZET010000000
VERSION     DAMZET010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.140"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..835)
                     /locus_tag="POB34_003653"
     CDS             complement(<1..835)
                     /locus_tag="POB34_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5920528.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVNLLRIPLFSLSICT
                     SV"
     gene            1012..1803
                     /gene="kdgR"
                     /locus_tag="POB34_003654"
     CDS             1012..1803
                     /gene="kdgR"
                     /locus_tag="POB34_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HDI5920529.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMRELSR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(1946..2185)
                     /locus_tag="POB34_003655"
     CDS             complement(1946..2185)
                     /locus_tag="POB34_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920530.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            2344..2487
                     /gene="mgrB"
                     /locus_tag="POB34_003656"
     CDS             2344..2487
                     /gene="mgrB"
                     /locus_tag="POB34_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HDI5920531.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            2562..2849
                     /locus_tag="POB34_003657"
     CDS             2562..2849
                     /locus_tag="POB34_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HDI5920532.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            3518..3661
                     /locus_tag="POB34_003658"
     CDS             3518..3661
                     /locus_tag="POB34_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HDI5920533.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            3674..3883
                     /gene="cspE"
                     /locus_tag="POB34_003659"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             3674..3883
                     /gene="cspE"
                     /locus_tag="POB34_003659"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDI5920534.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            4049..4858
                     /gene="rlmA"
                     /locus_tag="POB34_003660"
     CDS             4049..4858
                     /gene="rlmA"
                     /locus_tag="POB34_003660"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HDI5920535.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(4855..5421)
                     /gene="mntP"
                     /locus_tag="POB34_003661"
     CDS             complement(4855..5421)
                     /gene="mntP"
                     /locus_tag="POB34_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HDI5920536.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(5850..6308)
                     /locus_tag="POB34_003662"
     CDS             complement(5850..6308)
                     /locus_tag="POB34_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HDI5920537.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(6363..7214)
                     /gene="manZ"
                     /locus_tag="POB34_003663"
     CDS             complement(6363..7214)
                     /gene="manZ"
                     /locus_tag="POB34_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HDI5920538.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(7227..8027)
                     /gene="manY"
                     /locus_tag="POB34_003664"
     CDS             complement(7227..8027)
                     /gene="manY"
                     /locus_tag="POB34_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDI5920539.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(8090..9061)
                     /gene="manX"
                     /locus_tag="POB34_003665"
     CDS             complement(8090..9061)
                     /gene="manX"
                     /locus_tag="POB34_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDI5920540.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9524..>10896
                     /gene="yoaE"
                     /locus_tag="POB34_003666"
     CDS             9524..>10896
                     /gene="yoaE"
                     /locus_tag="POB34_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HDI5920541.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHL"
BASE COUNT         2870 a         2548 c         2680 g         2798 t
ORIGIN      
        1 aaacagatgt gcaaatagaa agtgaaaaca gcgggatacg cagtaaattc accggtagca
       61 gcggtacggg aagagaaagc tggcggcgaa taaagaaaat accaacaaca accattacca
      121 ccagttccgc agcaattaac gtcagcgatt gcccctgagc gaaaccactc aacgcagtga
      181 taagcaggcc gaaggttaac gcgttcatca cggcgctggg caggtcgaaa cggggtttac
      241 tggcgcgaga accattgggt ggcagaaaac gcatcgccag aagcagggcg ataataccta
      301 acggtacgtt gattaaaaat aaccatttcc aggatgcgat ggagaggatt gctgcagcaa
      361 ttgtcggccc ggcagcagaa gagacggcaa caataaacga gtttatgccc atccctctcc
      421 ccagaaaacg ttgtggatag atcaggcgga taagtgcggt attaacgctc atcaacgctg
      481 caccgccgaa accttgtatg acacgcgcaa gggtgagcat ttgcagcgaa tcagaaaggg
      541 cgcagaacag tgaagacaac agaaaaacga ccagaccgca tttataaata cgtcgatagc
      601 caaacatatc gcccagaaac gaaaacgaga gcagggagat gacaatggcg atttgatagg
      661 cgttcactac ccagatggaa ctggctggcg tggcatgaag gtccgtggcg attgttggca
      721 gggcgacgtt ggcgattgcg ccgtcaagga cagccatcga aatgccaatc acaatggtta
      781 atatcgcacc gtatcgctgg ggcaatggca ggccgtcggc ctgaactttt ggcataggaa
      841 ttttatatct ttggtgaata atcaacaggg gtatgattct agcactgata ttcacggtgg
      901 atgtcgcaga attgtaacta accggcaaaa gatggattgc gaccaccctg gtgcgaaatt
      961 ataataaaaa acagttctga tttttataaa acactcgcaa tgaggtgata aatggctaac
     1021 gcagatctgg ataaacagcc tgattctgta tcttccgtgc taaaagtttt tggcattttg
     1081 caggcgctgg gtgaagagcg cgaaataggg ataaccgagc tgtcgcagcg cgtcatgatg
     1141 tcaaaaagca ccgtttatcg ctttttacag accatgaaaa ccttaggtta tgtggcgcag
     1201 gaaggggagt cggagaaata ttccctgacc ctgaaattgt ttgaactggg cgctcgcgcg
     1261 ttacaaaacg tcgatttaat tcgtagcgca gatatccaga tgcgtgagct ctcccgcctg
     1321 accaaagaaa ctatccacct cggcgcactg gacgaagaca gtattgttta cattcacaaa
     1381 attgactcta tgtacaattt gcgcatgtat tcacggattg ggcgtcgtaa tccgctgtac
     1441 agcaccgcga ttggtaaggt actgctggca tggcgcgatc tcgatgaagt gaagcaaatt
     1501 cttgagggcg tggagtataa acgcagtacc gagcggacca tcaccagtac agaagcgtta
     1561 ttacccgttc tggaccaggt gcgcgagcag gggtatggcg aagataacga agagcaggaa
     1621 gaagggctgc gatgcattgc ggtaccggta tttgatcgct ttggcgtggt cattgccggt
     1681 ttgagcatct ccttcccgac gttgcgtttc tctgaagagc gtttacagga atatgtcgca
     1741 atgttgcata ccgcagcgcg caaaatttct gcccaaatgg gttatcacga ctatccgttc
     1801 tgatgagagt aagaacctgt cggaatatca aacagacagg ttctttattt agcatgagaa
     1861 aaataaagtt gaaggtgcgt tatattaaac gcgcttgcta taagaatatt ttactcatga
     1921 gtgagaatct ggttatttat tacccttaac cattatcgac cacgatattg cttttacgta
     1981 acagtgggca atctgttatc cccaagaaac cacttttagt gtgcaagtat tgtaccgtgc
     2041 tggtgcctct ggcagtcaga taggtacatt gcaaacctaa tcctgcggca ttctctttgc
     2101 ttccaatcaa aacgccatat ccgctgagta ataatcctat ccataccagt gctatcagca
     2161 taactgtgcg aatgatgaat cgcattacaa cctcttctct ttttatgttc gcttaatcgt
     2221 agcggcaata tgcgctgaag caagcgactc attccgaaaa agcacgaata tcgacatagt
     2281 taggcgctgt ttaactaacg catgctagtt taatgacata aggtaggtga aacggagatt
     2341 ggagtgaaaa agtttcgatg ggtcgttctg gttgtcgtgg tgttggcttg cttgctgctt
     2401 tgggcgcagg tattcaacat gatgtgcgat caggatgtac aatttttcag cggaatttgt
     2461 gccattaacc agtttatccc gtggtgatat cattttttca gcactagatt ttcttcctct
     2521 ccagcagtgg tagaatagcg cctctactca gaggtggtga aatgaacgaa gttgtaaatt
     2581 caggcgtgat gaacattgcg tctttggttg tatcggtggt ggttcttctt atcgggctca
     2641 tcttgtggtt ttttatcaat cgtgccagtt ctcggactaa cgaacagatt gaactgcttg
     2701 aggcgttgct ggatcagcaa aaacgtcaaa atgcactgtt acgtcgtttg tgcgaagcaa
     2761 acgaacctga gaaagcagat aaaaagaccg ttgagagtca aaaatcggtt gaagacgaag
     2821 atattattcg tctggtcgcc gaacgataat ccttcctcaa tcttgagtaa ccccgtcgtt
     2881 gaagggttac tcaattcatc ctgattatac gcactgccat ggtgcaactg cttcaaatta
     2941 aaactctggt ctgacttcaa ataattatca tattatttat ttgcgtaacg ggatatcaga
     3001 aaaagatatt ttcctcatta atttattagg atgtttacat cggatttgtg attaagcgtg
     3061 gtattattta ttagcgaaac gtttctctct tgattttttg ctcattcatc aatttttctt
     3121 attttaaatt ttcaatccct ttggggattg acttctcttt agggtaattg atagccgtta
     3181 actgactgtt ttatgagaaa aagtgatata actttttatt cattgcatag caaaaaatgt
     3241 gatattgcac gcactatgta ataacttctc ccactggcct ggaacaactg aacttattga
     3301 actatgttag aaactacgcc agtttaagta tctgcctgaa ctggcaaggt taagcacaat
     3361 gatatatcgg cgcgtattcc gttgcataag tgtgcaaaaa aagtggaaga cgtatcgaga
     3421 tttgtgcgtc tgatcgagac atgtttaaaa atggcttgcc ataattaacg ttgtatgtga
     3481 taacagattt cgggttaaac gaggtacagt tctgtttatg tgtggcattt tcagtaaaga
     3541 agtcctgagt aaacacgttg acgttgaata ccgcttctct gccgagcctt atattggtgc
     3601 ctcatgcagt aatgtgtcag ttttatctat gttatgcctg cgggcgaaga aaacaatcta
     3661 aggaattttt caaatggcaa agattaaagg tcaggttaag tggttcaacg agtctaaagg
     3721 ttttggcttc attactccgg ctgatggcag caaagacgtg ttcgtacact tctccgctat
     3781 ccagggtaat ggcttcaaaa ctctggctga aggtcagaac gttgagttcg aaattcagga
     3841 cggccagaaa ggtccggcag ctgttaacgt aacagctatc tgatcgaatc cactgatctg
     3901 aagtgtgaat acgcttcaat ctcgctataa agcctcgtcg aatgcgaggc tttttactat
     3961 gctttatctt cgctcctggc gttcggatat ttgcccgccg cgtgattcgc gttacacttg
     4021 cggcctttag tatcctgccg gagttgtcat gtctttttcc tgtccacttt gccatcagcc
     4081 tctttcgcgt gaaaaaaaca gctatatctg tccccagcga catcagtttg atatggcgaa
     4141 agaagggtat gtcaatctgc tgcccgttca gcataaacgg tctcgtgatc cgggcgacag
     4201 cgcggaaatg atgcaagcac gccgcgcatt cttagatgcc ggacattatc agccgctgcg
     4261 tgatgcaatt gtcgcccaac tgagggaacg gcttgatgat aaggccacgg cggtgctgga
     4321 tattggctgt ggtgaagggt attacacaca cgcatttgcc gatgcgttgc ccgaaatcac
     4381 cacgtttggt ctggatgttt cgaaggtagc gataaaagcg gcggcgaaac gctatccgca
     4441 ggtcactttt tgtgtcgctt ccagccaccg tttgccgttt tccgatacca gtatggacgc
     4501 cataatacgt atttacgcgc cgtgtaaagc agaagaatta gcacgagtag tgaagcccgg
     4561 cggctgggtc attactgcca cgccgggacc gcgacatttg atggagctga aggggctgat
     4621 ttacaatgaa gtacatcttc atgcacctca tgcagaacaa ctggaaggtt ttacattaca
     4681 gcagagtgcg gagttgtgtt atccgatgcg tcttcgcggt gatgaagccg tcgcattatt
     4741 gcagatgacg ccgtttgcct ggcgtgcgaa gccagaagtc tggcaaacac tggcagcaaa
     4801 agaagtgttc gactgccaga cggactttaa tattcacctc tggcagcgtt cttattaacc
     4861 gtggaagtgc gtccagagga tctggacgcc gatgccgatc agtaccagcc cgccgagaat
     4921 ttccgctttt ttcccaataa ttgagccgat aaagcgacca accatcatcc ctaatgttga
     4981 cataatcaag gttgcacaac caatggccaa tgcggtcgcg ataatgttga cctgcaggaa
     5041 agcaagacca acacccacag ccatggcatc caggctggtg gcaatcgcgg tggttaccag
     5101 tagccagaaa ccgtgtcgac ggcgcggctc ttcatcttca tcatctgcgc cacgaaaacc
     5161 ctcaataatc attcgcccgc cgaggaatat cagcagcaca aacgcaatcc agtggttcca
     5221 ttcaaggaca aaccggctgg ctaacatgcc cattccccag ccgatcagcg gcgtcagggt
     5281 ttcgacggca ccaaaaataa ggccggttcg caatgcttca gaaaatttcg gtttatggag
     5341 ggtggcacct ttaccgattg atgcagcaaa tgcatccatc gacataccaa acgcaagaag
     5401 aacagtagca gtgatattca tgacaatgtc ctgaccgggg tatccataaa acacatcatc
     5461 gcccccagca aacatgcata catgaacaac aagccgcgat ggttttcgcg ttcatgcagg
     5521 catagcgatt gatgtgcata tggtctcgcc tgactgaaag tattcagtcc gtacgtgcca
     5581 cgttttgcaa cgagtatgtt gacacgtaca tttccggaat ctggaaatcg gctactcccc
     5641 aaatgagggc gcaaccttaa catattttga gaatataaaa caactgttgg aatatattat
     5701 ttcattgctt tattgataac gatttacatt tagatttata tatcaatgaa acgttaaaat
     5761 aaataacaaa taagggaaga ggtaatatag cctaagctat gtctgattat taaaaataca
     5821 ctaaacatag ctttggctaa attcataact tattgagttg aaacgagaag cttataaatt
     5881 ttttccagat cgtcgatatt tcgaacgcgg attaacagcc gacgctgttc taattgcatc
     5941 accaacacgc catcttccga caagttcatc gctttgattc ggctatattc aatccagaca
     6001 ttggcgaaga aaaaaccttt ttgtttaaag atgatcttcg gaacgcggat ccagaatata
     6061 taaaaaccca tcagagccag tgcgcttaat aaccatgtcg ttattaacgc accatgattc
     6121 gtgacgttgt tatagataag aatgacaatc aatccgacga agataacgct atcgatgcga
     6181 ccacgccgga gcaaaggaat tgccagcagg gtggggccat tacggcgggg catgatgaac
     6241 tgatcgtaga tcgcgaaggc caggagtgcg gcgatgaaaa gaatcagtac caggtccgtg
     6301 attgtcatta atcctccaga taaaaaaacg gggccaaaag gccccggtag tgtacaacag
     6361 tcttacagtc ccagcaggcc gcaagcgtaa ccagcgatac cgatgacgaa gaagccaacg
     6421 atgatccaca gcgggttaac ttttttgcgc agtagccaca tacaagcaaa ggtcagcagc
     6481 agtggtacca ggcctggcat taactggtcc agaatagtct ggacagtagt aacgtgttct
     6541 ttgcccgtct ggtcagtaat gcgagagaca accagcggga tgttgacatg tgtccacttg
     6601 ttaaccaatg cccccatgac aaacaggccg aggatagacg ccccttccgt cagtttttgc
     6661 aggaagccac cacccatatc tttaacgata tcgatacctt tggagtaacc atacgctacg
     6721 ccgtagtaac gggttgccag acgcaccagg ttaaacagga tgaagaacag cagcggacct
     6781 aacaggctgc cgctcatcgc gataccggca cccagtgctg caaataccgg acgtacggtt
     6841 ccccagaaga tcgggtcgcc tacaccagcc agcggcccca tcaaaccgac tttgataccg
     6901 ttgatagcac cgtcgtcgat ctctgcgcca ttagcacgct gttcttccag cgccagggtt
     6961 acgccgagaa tcggcgcagc cacgaacggc tgggtgttaa agaactccag gtgacggcga
     7021 atagcttgtt tacgagcttc gttgttctca gggtagaggc gacgaattgc cggtaccata
     7081 gagaagcaga aacccagtgc ctgcatacgt tcgaagttcc atgaaccctg gaagaggtta
     7141 gaacgcagga agacgccacg aatatcactt tgagtgagtt ttttctcggt ggtagtttga
     7201 gttgtatcaa ccatttcgct cacctgttag tccagttcgt tatcgagatc gttgttacca
     7261 gctgcctgag caggcgcacc ggctacgcgg ttgtatttcg ggctaagttg gatgtagagc
     7321 actgccataa cagtaccaat cacacccaga gcaaccaggt taaagttggt gaatgctgcg
     7381 gttacgaagc cgaggtagaa gaacggcatc aggtagccag cacgcatcat gttgataacc
     7441 atcgcataac caaccaccac gatcatgcca ccggcgatat tcagaccatt ggtcaccact
     7501 tccggaatcg cattcagcat gttctgtact tcgctggtac caacagacag cgcaacgatg
     7561 acggccggaa tagccacacg cattgcttgc aggaacagag aagaaacgtg gatccaggaa
     7621 atcgctgtca ggttgccgtt atcagcagcc ttatccgcag cgtgctggaa agcaacggta
     7681 atagtacgaa cgatgatggt cagtacctgg cccgcagcgg ccagagggat tgccagtgcg
     7741 atacctgcac caatgctctg atgacctgcg ataaccagaa tagtagaaat gatggaagcc
     7801 agagcggcgt caggcgcaac tgcagcaccg atgttcatcc agcccagcgc gatcatttcc
     7861 agcgtaccac cgataataat accggttttc atatcgccaa gaacgatacc caccagggta
     7921 cacgcgatta gcggacggtg aaactgaaat tcatcgagga ttgatcccat acctgcgata
     7981 caggctacga taaataccag cacaatttga agagtggtaa tctccattgt acttctcctg
     8041 tttacgtaag acttaagtgt gaaaactgag tgataatcaa cacaatacgt tacttatcga
     8101 ttttgctgat cagatccatc attttcagtt tcggatcggt ggaaacctta cggacttcca
     8161 gctcaatacc gcgcgcattc agtttcttga acgcctcgat atctttttca tcaaccgaaa
     8221 ccgcgttatt cacctgggtt ttaccctgac ggaatgccat accaccgacg ttaacagagg
     8281 tgattttcac gccgccttca acgagacgct ctacatctgt tgggttggta aataacagca
     8341 ttacacgttc gccagcatat ttcgggttgt tgtagacgcg aatcattttg gcaacatcaa
     8401 ctacgtgtgc tgttacgccc ggaggtgcaa cctgggtgag cagtgtctta cgaacggtat
     8461 ccgcagccac ttcatcacta acaacaataa tacgggagac attggtttct ttggtccagc
     8521 gggtggcgac ctgaccgtga atcagacggt cgtcgatacg cgcaaggcca ataaccatgt
     8581 agtcgtttgg ccccattggt tttgccggag ttggagccgc ttttggtgct gcggcagcgg
     8641 gtgctggcgc ggctttttca accggtttgg ctttcagtgc tttcacgcct tcacggcctg
     8701 tttctactgc cagcgcaacc agttcatcaa agcttgggtc atcatcacga gccattaacg
     8761 tttccacgag cattggaatg ttaacgcctg caatgacttc ataatgctct ttgtcgacga
     8821 caatgcggct ggcagcattg aacgggctgc ctccccatgt atcaacgaga aacagcacgc
     8881 ctttagtggt gtcgagtttt gccaactgag cgttgtactt ttcaatcagc gtttcggcat
     8941 tttcacctgg aacgaaatcg atccagccga cgttttcctg ctcgcctaac agcatttctg
     9001 ccgttttaag caactgctct gcagcccaac catgtgtgcc tataacaata gcaatggtca
     9061 cttgctacct cctttattat cgttaacacc tcaacgtgcc agatgtattt ttgaatcgtc
     9121 gtccacaatc gaatcgattc agataagggc tacaaccaaa aacccctgcg tttcgcgatt
     9181 tattttagat atcgaaaaaa ttattttatg tgatgaagat ccgtaattta actttcgatt
     9241 agcagaaatt tcgaaaggta aaatatcctt gtcacattcg tttgcaaagg aaggtaaatc
     9301 tttgccaaat cagaggcgtc tctgatatgt ttaactcccg tttaacaacc atggagtata
     9361 gggcagtagc ccgcagtatg gatcgtcacc gacgtcattt cagcatcagg ccttttaacg
     9421 cctacctttc tggctctcta tgccgcacct ttcgtttgca ttttgtcgtt acgcctgcat
     9481 tatttctggc gtcgaatagc tattccttaa gcaggagctt gtcatggaat tcttaatgga
     9541 cccctcaatt tgggcggggc tactcacgct tgttgttctc gaaattgtgc tgggtatcga
     9601 taacctggtc ttcatcgcca ttcttgctga caaactgccg ccaaaacaac gcgataaagc
     9661 gcgtttgctg gggttatcac tggcgctgat tatgcgtctg gggctgctgt cgctgatttc
     9721 atggatggtc acgctgacca aaccgctatt taccgtcatg gatttctcct tctccggacg
     9781 cgacctgatt atgttgttcg gggggatatt cttgctgttc aaagcaacaa ccgaactgca
     9841 tgaacggctg gaaaaccgcg atcatgattc cggccacggt aaaggctacg ccagtttctg
     9901 ggtggtcgtc acacagattg tcatccttga cgccgtcttc tcgttggatg cggtaattac
     9961 tgcagtaggg atggttaacc atctgccggt gatgatggcg gcggtagtga ttgcgatggc
    10021 ggttatgttg ctggcatcca aaccgctgac gcgattcgtt aaccagcacc ccacggtggt
    10081 ggtgctctgt ctgagcttcc tgttaatgat tggtctgagt ctggtggcag aaggtttcgg
    10141 tttccacatt ccgaaaggtt acctgtatgc cgcgattggc ttctcgatca tcatcgaagt
    10201 gtttaaccag attgcgcgtc gcaactttat tcgccaccag tcgactttgc cgctgcgagc
    10261 gcgtactgcc gatgccatcc tgcgtttgat gggcgggaaa cgtcaggcca atgttcagca
    10321 cgatgccgat aacccgatgc cgatgccgat cccggaaggt gcatttgccg aagaagaacg
    10381 ttacatgatt aacggcgtac tgacgctggc gtcgcgttct ctgcgcggga tcatgacgcc
    10441 gcgcggtgaa ataagctggg ttgacgctaa tctcggggtc gatgaaatcc gcgagcaact
    10501 gctctcttca ccgcacagtc tgttcccggt atgtcgcggt gaactggatg aaatcatcgg
    10561 tattgtacgt gctaaagaac tgctggtggc gttggaagag ggcgttgatg tggcggcgat
    10621 tgcttcggcg tctccggcga ttatcgtccc ggaaaccctc gatccgatca acctgttggg
    10681 cgtgctgcgt cgtgctcgcg ggagctttgt tatcgtgacc aacgagtttg gtgtggtaca
    10741 aggtctggtc acgccgctgg atgtgctgga agccattgcg ggtgaattcc cggacgctga
    10801 cgaaacgccg gaaatcatta ctgatggtga cggctggctg gtaaaaggcg gtacagattt
    10861 gcatgccttg cagcaggcgc ttgatgttga gcacct
//
LOCUS       DAMZET010000141        10889 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.141, whole genome shotgun sequence.
ACCESSION   DAMZET010000141 DAMZET010000000
VERSION     DAMZET010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.141"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..262
                     /gene="ssrA"
                     /locus_tag="POB34_003667"
     tmRNA           <1..262
                     /gene="ssrA"
                     /locus_tag="POB34_003667"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            429..1658
                     /locus_tag="POB34_003668"
     CDS             429..1658
                     /locus_tag="POB34_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014641070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI5920542.1"
                     /translation="MAKIAKKLTDTEIKSTKPAEKEVDLFDGDGLLLRIAPLAKGGKK
                     NWYFRYAVPVTKKRTKVSLGTYPHLTLAKARALRDEYLSLLTNGIDPQVHNNQKANAL
                     KDATEHTFQAVAKKWLDEKVKTSGISQDHANDIWRSLERNIFSTLGDTPIKEIRPKML
                     KQHLEPIEKRGVLETLRRIISRLNEIFRYAATEELIEFNPADNLGQRFSKPKKQNMPA
                     LPPSELPRFLVALNNASIRLETRLLIEWQLLTWVRPGEAVRTRWSDIDIETGMWNIPA
                     EFMKMKKPHKVPLSKEALRVLDLMKVISGHREWVFPSIKAPLNHMHEQTANAAIIRMG
                     FGGELVAHGMRSIARTAAEESGKFRTDVLEAALAHSKKDEIIAAYNRAEYLTERVVLM
                     QWWSDYVSSQKCKVIAA"
     gene            1941..3497
                     /locus_tag="POB34_003669"
     CDS             1941..3497
                     /locus_tag="POB34_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI5920543.1"
                     /translation="MASENDKNHRVRVAQYLRMSTDHQQYSLHNQSEYIKDYAEKNNM
                     EIAYTYDDAGKSGVSIVGRHSLQQLLSDVEQKKIDIQAVLFYDVSRFGRFQNSDEAAY
                     YSFLFERNGVDLIYCSEPIPTKDFPLESSVILNIKRSSAAYHSRNLSEKVFIGQVNLI
                     KLGYHQGGMAGYGLRRLLVDENGIAKEILSFRKRKSIQTDRVILIPGPKNEIKIVNRI
                     YDLFIDNNVPEFIIAERLNEQNIPAENGTLWTRAKIHQILTNEKYIGNNIYNKTSSKL
                     KSRLVKNPKHEWVRCDKAYKPIISKKKYNKAQEIIQLRSIHLTNEDLLEKLKQKLESN
                     GKLSGFIIDEDDTGPSSSVYRTRFGGLLRAYTLIGYKPEHDYSYLKINEALRSFYSEI
                     IEDFKGEILKSNCHIDEYKYAPMLYINDEFLISVLVTKCIHMKSGKLRWKVRFDNSQK
                     ADITIVIRMNSQNISPLDFYIIPKIENEYNKMCMTETNNIRLDLYRFDNLDKLLQIIT
                     RMKVRELYAA"
     gene            3487..4383
                     /locus_tag="POB34_003670"
     CDS             3487..4383
                     /locus_tag="POB34_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDI5920544.1"
                     /translation="MLPEKNEFPIIQIEIAKIKFLNPRTRNKVVHEEIKESIKKRGLS
                     KPISVRAIDEGDFKYALICGQGRIEALVALGETIIPAIIRDVSEEDAYVMSLVENIAR
                     RRPRSNELLQVIKDMKIRGLSDSEISEITGYSSNWVSSINMLLDKGEHKLLSAVERGN
                     LPLYLAVQFARCETEEAQDILTEAYDKKLIKSRDIIKIKHILNQRTVGNKGAKAAGFY
                     YHKPSKRMTAEELIELYENSIAEHKSVYNNSKFIKTNLLIVNEIFNIIMMNKSFQHIL
                     EQENLSELPSQILTPVNKEVLK"
     gene            4380..5276
                     /locus_tag="POB34_003671"
     CDS             4380..5276
                     /locus_tag="POB34_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008502509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDI5920545.1"
                     /translation="MIQIRFGDNFIYLETNKLIPSKELLENVKRSHKYHQIVTSIESL
                     GIIEPIIVFYDKDKDVTKILDGHLRVEALKDLGIEKAPCILSSIDDAFTPNKQVNHIN
                     VVEEHRMIIKSLAKVSIEKLSAALGISVDAIKDKANVMNGIDPSVIAKLSDKPIPKAT
                     FDVLRKMKPIRQIEAVGTMINFDNYSKKFAMSILDATPASMIVNKGKNTPYKKDIKKT
                     ILRLEQEMATTSEETKKLQTEYGSDMLKFVIIQSYINKLLGNSKVLHWFLENEVDYLN
                     ELKRISRINSLDDKTLTENSKS"
     gene            complement(6392..6649)
                     /locus_tag="POB34_003672"
                     /pseudo
     CDS             complement(6392..6649)
                     /locus_tag="POB34_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611293.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6873..8420
                     /locus_tag="POB34_003673"
     CDS             6873..8420
                     /locus_tag="POB34_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDI5920546.1"
                     /translation="MARKPKEIKTDPLEVILWKAADKLRKNIDAAEYKHVVLGLIFLK
                     YISDSFESHYELLKAGKGEFAGADPEDKDEYTAYNVFFVPELARWNYLISKAKLPEIG
                     KMVDDAMELIEAGNPQLKGVLPKVYARQNLDATVLGELIDLIGNIALGDAKARSADVL
                     GHVFEYFLGEFALAEGKQGGQFYTPKSIVSLLVNMLEPYKGRVFDPCCGSGGMFVQSE
                     KFVEAHQGNIDDISIYGQESNQTTWRLAKMNLAIRGINSEHVRWNNEGSFLNDAHKDL
                     KSDFIIANPPFNVSDWSGEQLRGDARWQYGIPPAGNANFAWMQHFLYHLSPKGQAGVV
                     LAKGALTSKSSGEGDIRAALVKDANVIDCIVNLPAKLFLNTQIPAALWFMRRDRHNSN
                     QYRDRSKEILFIDARNLGHLINRRTKVLSDDDIKTIADTYHNWRNKGGDYEDVAGFCA
                     SVDIAEVAKLDYVLTPGRYVGLADEEDDFDFKERFTALKAEFEAQLEEEARLNRAIVD
                     NLAKVVL"
     gene            8417..9610
                     /locus_tag="POB34_003674"
     CDS             8417..9610
                     /locus_tag="POB34_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDI5920547.1"
                     /translation="MSRWRNFCITELADFCNAQRIPLSAMERAHKKGNFPYYGASGIV
                     DYIDNYIFDGSYILISEDGENLKSRKTPIAFEATGKFWVNNHAHVLKAKKPHLTALII
                     QYFSQLDLSPYLTGAAQPKLNKASLNLIPLYLPIDESEQKAIASILSSLKDKIDLLHR
                     QNKTLESMAETLFRQWFIEETHADWEITTLDCHITVAKGLSYKGAGLTTSDNGIPLFS
                     LNSVLEGGGYKSAGIKYYNGDFKERHIIKPGDIIVANTEQGHEYRLIGYPAIIPKTES
                     ELSIYTHHLFKISINDDSYLTNAFLYYLLCSKDMHEQVVAATNGSTVNQLSADGLQRP
                     EFKLPPECMVKEFTTQIDFFWKKISINNSQIKNIESLRDTLLPKLMSGEVRVQYAEEA
                     IASVA"
     gene            9669..>10889
                     /locus_tag="POB34_003675"
     CDS             9669..>10889
                     /locus_tag="POB34_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDI5920548.1"
                     /translation="MAKMTESDIEVMAIEHLQGLGYEYVYGPDIEPSGINPLRSYQQV
                     ILEDKVRTALQRINPHLSEQKCEEALKQVMQISSPDLMANNLTFHRLLTEGINIEVSK
                     DGNTQGELASLIDFNDPTNNAFLVINQLTIKEGNHTRRPDLILFINGLPLVVIELKNA
                     ADENATVTGAYNQIKTYQNQIPGLFNYNAFNVISDGLEAKAGTVSADFSRYMTWKTAN
                     GKTQATSTQPQLEVLLQGLLNPVTLLDIIRHFIVFEASKHEDSKGIISIRTVKKMAAY
                     HQYYAVNAAVLSTIRASAVNADSPSAEVALRQQGRNSKDLVNAQKTGDRKAGVVWHTQ
                     GSGKSLSMVFYTGKIVLALDNPTVVVITDRNDLDDQLFGTFSSATQLLRQTPKQANNR
                     EELKEYLRVASGGVV"
BASE COUNT         3590 a         2132 c         2207 g         2960 t
ORIGIN      
        1 aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc ctccgctctt
       61 aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc tgcctggggt
      121 tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg cagctggcaa
      181 gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc ggacgcgggt
      241 tcaactcccg ccagctccac caaatattga tgtactgaag ttcagtaaag tctactaagc
      301 ccgcacagca caagctctgc gggctttttt acgtctattg tagtctagtg agaattgctg
      361 agaactacga gttatggcac cctgaatggg acccacgaag atgggtccta aaatcgaggg
      421 tcccaaacat ggccaaaatc gctaagaagc tcactgacac tgaaatcaaa agcaccaaac
      481 ctgccgaaaa agaggttgac ctttttgacg gcgatggttt gctgttgcga atcgctcccc
      541 tggcgaaggg agggaagaaa aattggtatt tcagatatgc agtgcctgtg accaaaaagc
      601 gaactaaggt gagcttagga acctatcctc accttacact tgcgaaggca cgagctttac
      661 gtgatgaata cttgtcgttg cttacaaatg gtatagaccc ccaagttcat aacaaccaaa
      721 aagccaatgc actgaaagat gccacggaac atacatttca agcagtagcc aagaagtggc
      781 ttgatgagaa agtcaaaacg tcaggcatct cccaagatca tgctaacgac atctggcgaa
      841 gcctagagag aaatatcttt tccacattgg gtgatacccc aattaaggag attcgcccta
      901 aaatgcttaa acagcattta gaacccatag aaaaacgagg tgtccttgaa acacttcgcc
      961 gcatcatatc ccgcctgaat gaaattttcc gctatgcagc aacagaagaa ctcatagaat
     1021 tcaacccggc agacaacctg gggcaacggt tcagcaagcc aaaaaaacag aatatgccag
     1081 cattaccccc ttccgaactc cctcgcttct tggttgctct aaacaatgct tctatccgtt
     1141 tggaaacaag gctactgatt gagtggcaac ttctcacatg ggttcgccca ggtgaagctg
     1201 ttcgcacaag atggtcagat attgatattg aaactggcat gtggaacatc ccggcggagt
     1261 ttatgaaaat gaagaagcct cacaaagttc cactgagcaa agaagctttg cgagttttgg
     1321 atttaatgaa agtcatcagc gggcatagag agtgggtgtt ccccagtatc aaagctccac
     1381 tcaatcacat gcatgaacaa acagctaatg cggccataat ccgtatgggt ttcggaggtg
     1441 agcttgtagc tcacggtatg cgatccattg ctagaacggc tgctgaggag tctggcaagt
     1501 ttaggactga tgtcttagaa gccgcccttg cccactcgaa gaaagatgaa ataattgcag
     1561 cctacaatcg tgcagagtat ctcactgaac gggtggttct catgcaatgg tggagtgact
     1621 atgtttcgtc tcaaaaatgc aaagttattg ccgcataact ctcccatgat ggattaacta
     1681 tcttgattta gttaaagaat taataatcac accattaacc tatgtggact aagcatagcc
     1741 atttacaaat ggggacattg agtccacata acgaaagctg tcggtcatat cagctaaata
     1801 attcacatct cttctcgatt atcatacacc tcgaagatct accaaattcg ctctaatcaa
     1861 tgataaaaca gttgaattcg gttgaaattt gatcaatttt atccactaca ttgtattgaa
     1921 tcatccatga ggtttcgtgc atggctagcg aaaatgacaa aaatcataga gttagggtcg
     1981 cgcagtactt gaggatgtct accgaccatc agcaatattc tttacataat cagtccgaat
     2041 atatcaaaga ttatgctgaa aagaacaata tggaaatcgc ttatacctac gatgatgcag
     2101 gtaagagcgg agtcagtatc gtaggcaggc attctttgca gcagttactt agcgatgtag
     2161 aacaaaagaa aatagatata caggctgtat tattttatga tgtgagccgt tttggtcgtt
     2221 ttcaaaatag tgatgaagcg gcatattatt cctttctatt tgagagaaat ggtgtagatc
     2281 ttatatattg ttccgaacct atacccacta aagatttccc tttagagtcc tctgttatac
     2341 tgaatataaa aagatctagt gctgcatatc acagcaggaa tctatctgaa aaggtattta
     2401 tagggcaagt aaatttaata aagcttggtt atcatcaagg cggtatggct ggttatgggc
     2461 tgagacgtct tttagtagac gaaaatggca tagctaaaga aatattgagt ttccgcaaaa
     2521 gaaagagtat tcaaacagat agggtaatat taattccggg accaaaaaat gaaataaaaa
     2581 ttgtaaatag aatatatgat ctctttatag ataataacgt cccagaattc attattgctg
     2641 agagattaaa tgaacagaac atacctgcag aaaatggaac attatggact cgtgcaaaaa
     2701 tacatcaaat tttgacaaat gaaaaatata ttggaaacaa catatataac aaaacctcat
     2761 ctaaattaaa aagtaggctt gtaaaaaacc ccaaacatga atgggttaga tgtgacaagg
     2821 catataaacc tattatttca aagaaaaaat acaataaagc tcaagaaata attcagctcc
     2881 gatccattca tttgactaat gaagatctat tagaaaaact aaaacaaaaa ttagaatcta
     2941 atggaaaact atcaggcttt atcattgatg aagatgatac aggcccttca tcttctgttt
     3001 atagaacccg atttggtggt cttttaagag catatacttt gattggttat aagccagaac
     3061 atgattacag ctatctcaaa ataaatgaag cactaagatc attttactca gagataattg
     3121 aggattttaa gggtgaaatt ttaaaaagta actgtcatat agacgagtat aaatatgccc
     3181 caatgcttta catcaatgat gagtttttaa tttccgtcct tgttactaaa tgcatacata
     3241 tgaaatcagg taaacttaga tggaaagtcc ggtttgataa ctcacagaaa gcagacataa
     3301 caattgttat acgaatgaat tcacaaaata tttcacctct tgatttttat atcataccaa
     3361 agattgaaaa cgaatataat aaaatgtgca tgacggaaac aaacaacatt cgattagatc
     3421 tctatagatt tgataatctt gataaacttc tacaaattat tactcgcatg aaagtgaggg
     3481 aactatatgc tgcctgaaaa aaatgaattc ccaataattc aaattgagat tgcaaaaata
     3541 aaatttctta acccacgaac aagaaataag gtagtgcatg aagaaataaa ggaaagcata
     3601 aaaaaaagag gattaagcaa gccaataagc gtaagagcta ttgatgaagg cgatttcaaa
     3661 tatgctttaa tttgtggtca agggagaata gaggctctcg ttgcattagg tgaaactatt
     3721 attccagcaa ttataagaga tgtatcagaa gaagatgctt acgttatgag tttagttgaa
     3781 aacattgcaa ggagaagacc acgttctaat gagttattac aggtgattaa agacatgaaa
     3841 atcagaggac tttcagactc cgaaataagt gagattactg gatattcatc gaactgggtg
     3901 agcagtatta atatgctact tgataaggga gagcataaac ttctctcagc agtcgaacgg
     3961 ggtaatttgc ctctgtatct cgcagtgcaa tttgcaagat gtgaaactga ggaagcacaa
     4021 gatattctta ccgaagcata tgataaaaaa ttaataaaaa gtcgggacat tataaagata
     4081 aaacacattc taaatcaaag aacagttggg aataaaggtg caaaagcagc cggattttat
     4141 tatcacaaac catcaaaaag gatgactgca gaggagttga ttgagcttta tgaaaatagt
     4201 atcgctgaac ataaatctgt ttataacaac tcaaaattca taaaaaccaa cctactaata
     4261 gtaaatgaga tttttaacat cataatgatg aataaaagct ttcaacatat acttgaacaa
     4321 gagaatcttt cagaactacc atctcagata ttaaccccag taaacaaaga ggtattaaaa
     4381 tgattcagat acgttttggc gataatttta tttacctgga aactaataag ttaattccat
     4441 ctaaggaatt attagaaaac gtaaagcgaa gccataaata tcatcaaata gttacctcta
     4501 tcgaaagctt aggtattatt gaaccaataa tagtattcta tgacaaagat aaagatgtca
     4561 ctaagatact tgatggccat ttaagggttg aggctttaaa agacttaggt atagaaaaag
     4621 ctccatgtat actttcgagc atagatgatg ctttcactcc taacaaacaa gtgaatcata
     4681 taaatgtagt tgaagaacat agaatgataa ttaagtctct ggcaaaagta tcaattgaaa
     4741 aacttagtgc tgctttgggc atatctgttg atgccataaa agataaagcg aatgtgatga
     4801 acggcataga tccaagtgta attgcgaaac tttctgataa acctatacct aaggctacat
     4861 ttgacgtttt gaggaaaatg aagccaattc gccaaattga agcagtcggt acaatgatta
     4921 attttgataa ttatagtaaa aaatttgcaa tgagcatctt ggatgcaaca ccggcatcga
     4981 tgatagtaaa taaagggaaa aacactccct ataaaaagga cataaaaaaa accatacttc
     5041 gtctggaaca agaaatggca acaacttcgg aagaaacgaa aaagcttcaa accgagtatg
     5101 gttcagatat gttgaaattc gttataatcc agtcatatat taataaatta cttggcaatt
     5161 ctaaagttct tcattggttc ttagaaaacg aggttgatta tcttaatgag ttaaaaagaa
     5221 tttctagaat aaattctttg gatgataaga ctcttactga aaacagcaag tcatagtcat
     5281 ggttttatat agcctaaata aaccaagaag tatacttaca caacgacctt cacgccgaag
     5341 aagctgtcag atacgccagc aaccaaagca ttacgtttaa gtgtcagtca ttgatacgaa
     5401 gggataactg cgtatcagca gccggacatt gagtcgaata aaggctggta gagagggaca
     5461 gttctgtgtc accgggagat tcgtcagaaa catcagcggc agaacagatg aaccagcaat
     5521 tggtagtgca cagaaaactc tccagtattt aattattcaa cttaacacta aaaaaaatca
     5581 catagttaat atcataattt ttctttggtc tacgtatgcg agagatattg tggctttaac
     5641 cgggtattag gagtcgtttc agataaaaga tgttcttcga ttcgactgga cttggtaata
     5701 gcaataagta atcgttaata ttgaaggacg aaaggctact tcaaaacaaa gtaacctttc
     5761 caatattccc atctatggga caagtggtac attaactgca catagtgcag gatacatatg
     5821 acataaaact ttttatttat ggcatacatt ataaataact tgtattttat gaatgtcaat
     5881 aggttcttgt ttaatacttt taaaattatt aattgattgt ttttattata gcttcgcttg
     5941 tacaagcgaa gatcctattg ttcttgaatg aataatgcca attataagag aagcaagggc
     6001 taactctgcg aagccgctgc gcttgcttct tcgagttccc cttgcttttc tgcccttcgg
     6061 ttataaaaga agccaaactg ctaccaacct tgaatctcta tatttttgaa tagacaaaca
     6121 ttacccgctc ccttaatcac cactatcaaa aataaaaaat catcttacaa ataaccccat
     6181 acacatcatt ttagattgcg accttattaa attaaaaatt caaccttaga tggaatttaa
     6241 tgtacaagga atgtcctact gtaacggaga attttaaaca ttaaaaatta caactatggt
     6301 ttgtcatagc ctgtacatct attcacctca tgcatatgca ctaagaaagc ttttttgaat
     6361 actggagttg tccctgctaa aacggacacg gctaattaac tgctaatatg gcatcacgat
     6421 attcgcgtgg cgatttcatg ttcaacccct tgtgtggatg cagattatta taatcctcta
     6481 tctattctgc cattgtttcc atcatttaga ctgcatccag cagatcatct acgtacacat
     6541 aatcccgctt gaacgtcttt acaaacgatt ctgccatgcc gttacttccc ggactgcgta
     6601 ctggtgtagt acacacgatg aaccacagtg aactagcgaa tgtgcgcgtg gcatcggctg
     6661 tgcagcaatt atgccaatga cactggctcc attgacggca atgacctgac atctacaatc
     6721 ccgctacaaa gcatttactc catgagtcat tgcacttgct gagctacacg aacacactaa
     6781 aaccagataa taattaatcg ctgtataagc tcgtaacaag cggaacttta aggcaaaata
     6841 gggccatctt cccccttcat aaggttacgc taatggccag aaaacctaaa gaaatcaaaa
     6901 cagacccgtt agaggtcatc ctgtggaaag cggcagacaa actgcgtaaa aacattgatg
     6961 cagccgagta caagcacgtc gtactaggcc tcattttcct taagtacatt tctgattctt
     7021 ttgaatctca ttatgagttg ctgaaagccg gtaaaggcga gttcgcaggt gctgatccgg
     7081 aagacaaaga cgagtacacc gcttacaacg ttttctttgt ccctgagctt gcacgttgga
     7141 actatctgat ttctaaggcc aagcttcctg aaatcggtaa gatggttgat gatgcgatgg
     7201 agcttatcga agcgggcaat ccacagttaa aaggtgtgtt gccgaaagtc tatgcccgac
     7261 aaaacctcga cgccactgtt ctgggtgagc tgatagatct gattggcaac attgcactag
     7321 gagatgccaa agcacgctct gctgacgttt taggtcacgt attcgaatac ttcctaggtg
     7381 aatttgcact ggcagaaggc aaacaaggcg gccagttcta tacgccaaaa tctattgtaa
     7441 gcctgctggt taacatgctg gaaccctata aaggccgtgt ctttgatccc tgctgtggtt
     7501 ctggtggtat gttcgttcag tcagaaaaat ttgtagaagc acatcaggga aatattgacg
     7561 atatttcgat ctatgggcag gagtccaacc agaccacttg gcgtctggca aaaatgaacc
     7621 tggcaattcg tgggattaat tctgaacacg ttcgctggaa taatgaaggt tcatttctta
     7681 acgatgctca caaagatttg aaatctgatt ttatcatagc taacccaccg tttaacgttt
     7741 ccgactggtc tggtgagcag cttcgtggtg atgcccgctg gcaatatggc attccacctg
     7801 ctggcaacgc taactttgca tggatgcaac attttttgta tcacttgtct cctaaagggc
     7861 aagcgggtgt tgttctggca aaaggggcat taacttctaa aagctctggt gaaggtgata
     7921 ttcgtgctgc actggtaaaa gatgccaatg tgattgactg tatcgtcaac ttaccggcaa
     7981 agctgttcct gaatactcag atccctgctg cattatggtt tatgcgccgt gaccgtcata
     8041 acagcaacca gtatcgtgat cgtagtaaag aaattctgtt tattgatgcc cgtaatctcg
     8101 gtcatttaat taatcgtcgt accaaagtac tttctgacga cgatataaaa accatcgctg
     8161 atacctatca taactggcgt aacaaaggtg gcgactacga agacgtcgct ggtttctgtg
     8221 cgtcagtaga cattgctgaa gttgctaaac tcgattatgt gctgacacct ggccgttatg
     8281 ttggtcttgc tgatgaagaa gacgatttcg actttaaaga acgctttacg gctcttaaag
     8341 ctgagtttga agcgcaactg gaagaagagg cgcgtctgaa cagagccatc gttgataatc
     8401 tggcgaaggt ggttttatga gtaggtggag aaatttttgt ataactgaat tagcagattt
     8461 ttgcaacgca caaagaattc ctttgagtgc tatggaacga gcccataaga aaggaaattt
     8521 tccatattat ggagcgtcag gaattgtcga ttatatcgac aattatattt ttgatggcag
     8581 ctacatcctt atttctgaag atggagaaaa cttaaagtcc agaaaaacac ctatagcatt
     8641 tgaagctact ggaaaatttt gggttaataa tcacgctcat gtccttaaag ctaaaaagcc
     8701 ccatttaacg gcattgataa tccaatattt ttcacaactt gatctatctc cttatttaac
     8761 aggtgctgct caaccaaaat taaataaagc atcgctaaat ttgattcctc tttacttacc
     8821 aattgatgaa tctgaacaaa aagccatcgc ctctattttg tcctctctta aagacaaaat
     8881 agacctgctt catcgccaga ataaaactct ggaatccatg gcagaaaccc tatttagaca
     8941 gtggtttatt gaagagacgc atgcggattg ggaaataacg actcttgatt gtcatattac
     9001 agtagctaag ggattaagct acaagggagc cggccttacc acgtcagata acggcattcc
     9061 actttttagt ttgaactcag tacttgaggg cggtggttat aagagtgcag gtataaaata
     9121 ctacaatggt gattttaaag aaaggcatat tataaagcct ggggatatca ttgtagccaa
     9181 tacagaacag ggccatgaat atcgactcat agggtatcct gctataattc caaaaacaga
     9241 aagcgaatta tccatatata cgcatcattt atttaaaatc agcatcaatg acgatagtta
     9301 cttaaccaat gcttttctgt actatttact ttgctccaaa gatatgcatg agcaagttgt
     9361 tgctgcaact aacggttcaa ctgtaaatca gctgtcggct gacggattac aacgcccaga
     9421 attcaaactc cccccggagt gtatggtgaa agaattcact acacagattg atttcttctg
     9481 gaaaaaaata agtattaaca actcacaaat taaaaacata gaatctctgc gcgacactct
     9541 ccttcccaaa ctgatgagcg gcgaagtacg ggttcagtat gcggaagaag caatcgcatc
     9601 agtagcataa aataattatg cgggtgtaca gcccgctctc tggttaagcg gcaataagga
     9661 cagtacgcat ggcaaagatg accgaatccg atattgaagt aatggcaatt gagcacctgc
     9721 aagggctggg ctatgagtat gtttacggcc cggacattga acccagtgga atcaatccgt
     9781 tacgtagcta tcagcaggtt atccttgaag ataaagtgcg tacagcattg caacgaatta
     9841 acccgcacct tagtgagcag aagtgtgaag aagctctaaa acaggtgatg cagatcagct
     9901 cacctgacct aatggcaaac aatctaacct ttcatcgcct tttgaccgaa ggaatcaata
     9961 tcgaagtcag caaggatggt aatacacaag gagaactggc cagcctgatc gactttaacg
    10021 atcccactaa taatgcattt ctggtcatta atcagctcac catcaaagaa ggcaaccata
    10081 cccgccgacc tgatcttatt ttattcatca atggcctgcc attagtcgtt atcgaactta
    10141 aaaatgccgc tgacgaaaat gcaacggtaa caggtgccta taatcagatt aaaacctatc
    10201 agaaccaaat ccccggtctg tttaactaca atgcttttaa tgtgatatct gacgggctgg
    10261 aagccaaagc ggggacggtt tctgccgatt tcagtcgtta tatgacgtgg aaaactgcta
    10321 acggtaaaac gcaagccacc agtacccaac cacagcttga agttttatta caggggttgc
    10381 ttaatcctgt aacgctgctg gatataatcc gccactttat cgtgtttgag gccagcaaac
    10441 atgaagacag caaagggatt atcagtatcc gtactgttaa aaaaatggcg gcttatcacc
    10501 agtactacgc ggtcaatgca gcagttcttt ccactattcg tgcctcagcg gtgaatgcgg
    10561 actccccctc tgccgaagtg gcactgcgcc agcagggacg taacagtaaa gatcttgtta
    10621 atgcgcaaaa aaccggagat cgcaaagcgg gcgtagtctg gcatactcag ggttccggta
    10681 aatcgctttc gatggtgttt tataccggga aaattgtgct ggcgctggat aatccgacag
    10741 ttgttgtgat tactgaccgt aacgacttag atgatcagct attcggtacg ttctcttccg
    10801 cgacccagct acttcgtcag acaccaaaac aggccaacaa ccgggaagaa ctcaaagaat
    10861 atttgcgtgt cgcctctggc ggtgtggtg
//
LOCUS       DAMZET010000142        10847 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.142, whole genome shotgun sequence.
ACCESSION   DAMZET010000142 DAMZET010000000
VERSION     DAMZET010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.142"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(167..463)
                     /locus_tag="POB34_003676"
     CDS             complement(167..463)
                     /locus_tag="POB34_003676"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920549.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            747..2378
                     /gene="oppA"
                     /locus_tag="POB34_003677"
     CDS             747..2378
                     /gene="oppA"
                     /locus_tag="POB34_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HDI5920550.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2464..3384
                     /gene="oppB"
                     /locus_tag="POB34_003678"
     CDS             2464..3384
                     /gene="oppB"
                     /locus_tag="POB34_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HDI5920551.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3399..4307
                     /gene="oppC"
                     /locus_tag="POB34_003679"
     CDS             3399..4307
                     /gene="oppC"
                     /locus_tag="POB34_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HDI5920552.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            4319..5332
                     /gene="oppD"
                     /locus_tag="POB34_003680"
     CDS             4319..5332
                     /gene="oppD"
                     /locus_tag="POB34_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HDI5920553.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            5329..6333
                     /gene="oppF"
                     /locus_tag="POB34_003681"
     CDS             5329..6333
                     /gene="oppF"
                     /locus_tag="POB34_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HDI5920554.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6386..6715)
                     /locus_tag="POB34_003682"
     CDS             complement(6386..6715)
                     /locus_tag="POB34_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HDI5920555.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6750..8210)
                     /gene="cls"
                     /locus_tag="POB34_003683"
     CDS             complement(6750..8210)
                     /gene="cls"
                     /locus_tag="POB34_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDI5920556.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8353..8526
                     /locus_tag="POB34_003684"
     CDS             8353..8526
                     /locus_tag="POB34_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HDI5920557.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8581..9834)
                     /gene="kch"
                     /locus_tag="POB34_003685"
     CDS             complement(8581..9834)
                     /gene="kch"
                     /locus_tag="POB34_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HDI5920558.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(10134..10430)
                     /locus_tag="POB34_003686"
     CDS             complement(10134..10430)
                     /locus_tag="POB34_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HDI5920559.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            10654..>10847
                     /locus_tag="POB34_003687"
     CDS             10654..>10847
                     /locus_tag="POB34_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="membrane spanning protein in TonB-ExbB-ExbD
                     complex; transduces proton motive force of the cytoplasmic
                     membrane to outer membrane transporters; involved in the
                     transport of ron-siderophore complexes, vitamin B12 and
                     colicins; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HDI5920560.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPP"
BASE COUNT         2855 a         2525 c         2632 g         2835 t
ORIGIN      
        1 cttaattaat tcctttcaaa tgaaacggag ctgccatgct ccgtttactt cgtcattatt
       61 tttactttgt tcccgcgcag ttatcaaaag caaaaggaat aggtaaaaat attcttctca
      121 aattacagtt agttataagg atttccttaa ctgcttctcc tcaccatcat gttattttcg
      181 ccacatcata atcctgggct tgctgaagaa taattgaaat gatattagta attccactgc
      241 ctttggtaga ggaaagtgct aaataataat caattgttaa attattgtgc atttcactac
      301 tggaactgta atcagaaaag atagacatgc ttagccaatc tctatttgat tgaattgaaa
      361 gatgtttgtt aaggcatgga tgcaagctat agattctgat acggtcaata aaagagaatt
      421 gcttaacaat tttgcaaaat gtattggcga gtaagaaccg catttggtac tttccgggca
      481 accgccagac gattctttat tggtaatgag aataattaac aattaaagag cgtcgcgaaa
      541 gaataatgtg tctcgacagg ggagacacag tacgaatcga cataaggtga tcgtctgaat
      601 caccagaata aataaagtcg gtgatagtaa tacgtaacga taaagtaacc tgacagcaga
      661 aagtctccga gcctgtgcag ggtcccaatc cgggattaca catgctggtt aataccagta
      721 attataatga gggagtccaa aaaacaatga ccaacatcac caagagaagt ttagtagcag
      781 ctggcgttct ggctgcgcta atggcaggga atgtcgcgct ggcagctgat gtacccgcag
      841 gcgtcacact ggcggaaaaa caaacactgg tacgtaacaa tggttcagaa gttcagtcat
      901 tagatccgca caaaattgaa ggtgttccgg agtctaatat cagccgagac ctgtttgaag
      961 gcttactggt cagcgatctt gacggtcatc cagcacctgg cgtcgctgaa tcctgggata
     1021 ataaagacgc gaaagtctgg accttccatt tgcgtaaaga tgcgaaatgg tctgatggca
     1081 cgccagtcac agcacaagac tttgtgtata gctggcaacg ttctgttgat ccgaacactg
     1141 cttctccgta tgccagttat ctgcaatatg ggcatatcgc cggtattgat gaaattcttg
     1201 aagggaaaaa accgattacc gatctcggcg tgaaagctat tgatgatcac acattagaag
     1261 tcaccttaag tgaacccgtt ccgtacttct ataaattact tgttcaccca tcaacttcac
     1321 cggtgccaaa agccgctatc gagaaattcg gcgaaaaatg gacccagcct ggtaatatcg
     1381 tcaccaacgg tgcctatacc ttaaaagatt gggtcgtaaa cgaacgaatc gttcttgaac
     1441 gcagcccgac ctactggaac aacgcgaaaa ccgttattaa ccaggtaacc tatttgccta
     1501 ttgcttctga agttaccgat gtcaaccgct accgtagtgg tgaaatcgac atgacttata
     1561 acaacatgcc gatcgaattg ttccagaagc tgaaaaaaga gatcccggac gaagttcacg
     1621 ttgatccata cctgtgcact tactattacg aaattaacaa ccagaaaccg ccattcaacg
     1681 atgtgcgtgt gcgtactgca ctgaaactag gtatggaccg cgatatcatt gttaataaag
     1741 tgaaagcgca gggcgacatg cccgcctatg gttacactcc accgtatact gatggcgcaa
     1801 aattgactca gccggaatgg tttggctgga gccaggaaaa acgtaacgaa gaagcgaaaa
     1861 aactgctggc tgaagcgggt tataccgcag acaaaccgtt gaccatcaac ctgttgtata
     1921 acacctccga tctgcataaa aagctggcga ttgctgcctc ttcattgtgg aagaaaaaca
     1981 ttggtgtaaa cgtcaaactg gttaaccagg agtggaaaac gttcctcgac acccgtcacc
     2041 agggtacttt tgatgtggcc cgtgcaggct ggtgtgctga ctacaacgaa ccaacttcct
     2101 tcctgaacac catgctttcg aacagctcga tgaataccgc gcattataag agcccggcct
     2161 ttgacagcat tatggcggaa acgcttaaag tgactgacga ggcgcagcgc acagctctgt
     2221 acactaaagc agaacaacag ctggataagg attcggccat tgttcctgtt tattactacg
     2281 tgaatgcgcg tctggtgaaa ccgtgggttg gtggctatac cggcaaagat ccgctggata
     2341 atacctatac ccggaatatg tacattgtga agcactaatg gcaatacgtg gggcaggagt
     2401 gtcctgctcc acggtgtctg atttttatcg cattacagaa ggcacaggcc agaaggtagg
     2461 gcaatgttaa aatttattct acgtcgctgt ctggaagcga ttccgacgct atttattctt
     2521 attactattt cgttctttat gatgcgcctc gcgccgggaa gcccttttac cggcgaacgt
     2581 actttaccgc cagaagtgat ggccaatatc gaagcgaaat atcatcttaa tgatccgatc
     2641 atgacacagt atttcagcta cctgaaacaa ctggcgcacg gtgatttcgg tccatcgttt
     2701 aaatataaag attattcggt caatgacttg gttgcatcca gttttcccgt ttctgccaaa
     2761 ctgggagccg cagcattttt ccttgcggta atactgggtg ttagtgctgg cgttattgcc
     2821 gcattaaaac aaaacaccaa atgggactat accgtgatgg ggctggcaat gaccggggtt
     2881 gttatcccca gttttgtggt tgcaccatta ttagtcatga tatttgcgat cattttgcat
     2941 tggctgccgg gcggtggctg gaatggtggg gcgcttaaat tcatgatatt gccgatggtg
     3001 gcgttgtcac tcgcttatat cgccagtatt gcgcgtatta cccgtggctc tatgattgaa
     3061 gtattacact ccaattttat tcgtactgcc cgggcgaaag ggttacctat gcggcggatc
     3121 attttacgtc acgcattaaa acctgctctg ttacccgtgc tctcttatat gggccccgca
     3181 tttgtcggca ttattaccgg ttctatggtt atcgaaacca tttatggttt gccggggatt
     3241 ggacaactgt tcgttaatgg cgcattgaac cgtgactatt ccttagtgtt aagcctgacc
     3301 atcctggttg gtgctttaac cattttgttt aatgccattg tcgatgtgct atatgcggtt
     3361 atcgacccga aaatccgtta ctgatactgg agctcgcgat gatgttaagt aagaaaaaca
     3421 gcgagacgct ggaaaatttc agtgaaaagc tggaggtcga agggcgcagc ttgtggcagg
     3481 acgcacgtcg acgttttatg cataaccgtg cggcggttgc cagtctgata gtgctggtgc
     3541 tgatcgcgtt atttgtaatc ctggcaccga tgctttccca gtttgcctat gacgatactg
     3601 actgggcgat gatgtccagc gccccggata tggagtccgg tcactacttt ggtactgact
     3661 catccggtcg cgacctgctt gtgcgcgttg cgattggcgg gcgtatctca ctcatggtcg
     3721 gtgttgctgc ggcactggtg gctgtggtcg tcgggacact ttatggttcg ctttccggtt
     3781 atctgggcgg taaagtggat tcggtaatga tgcgtctgct ggaaatcctc aactccttcc
     3841 cattcatgtt cttcgtcatt ttgctggtga cctttttcgg tcaaaacatc ctgctgattt
     3901 tcgtggcgat aggcatggtt tcctggctgg atatggctcg tattgtgcgt gggcaaaccc
     3961 tgagtctgaa gcgcaaagag tttattgagg cggcacaagt tggcggtgta tcgacatcgg
     4021 gcattgttat tcgccacatt gtgccgaatg tactcggtgt ggtggtggtc tacgcatcac
     4081 ttctggtgcc cagcatgatc ctctttgaat ctttccttag cttcctgggg ttgggtacgc
     4141 aagagccgtt aagcagctgg ggtgcattgc tgagtgatgg cgcgaactcg atggaagtct
     4201 ctccatggtt attgttgttc ccagcgggat tcctcgtggt gacgctgttt tgtttcaact
     4261 ttatcggcga tggcttgcgt gatgccctcg acccgaaaga tcgttaagga gtgcagccat
     4321 gagcgtaatt gaaactgcaa ctgtgccgct cgcacaacaa caggctgacg cactgctgaa
     4381 cgtgaaagat ttgcgtgtca cctttagtac cccggacggc gacgtcacgg cggtcaatga
     4441 tttgaatttt tccctacgtg ccggagaaac gctgggtatt gtaggtgagt ctggttcggg
     4501 taaatcgcaa actgcatttg cgttgatggg cctgttggct gccaacggac gtattggcgg
     4561 atcggcaacc ttcaatgggc gtgaaatcct caatttgcca gagcatgaac tcaataaact
     4621 gcgcgctgaa caaatctcaa tgatttttca ggacccaatg acttcgttga atccctatat
     4681 gcgcgtcggt gagcagttaa tggaagtgct gatgctgcat aagaacatga gcaaagctga
     4741 ggcgtttgaa gagtcggtgc ggatgctcga tgcggtaaaa atgccggaag cgcgtaaacg
     4801 catgaaaatg tacccgcacg aattttctgg cggcatgcgt cagcgagtca tgattgcgat
     4861 ggcattgcta tgtcgaccta agctactgat tgcggatgaa cccactactg cgctggacgt
     4921 caccgtacag gcgcagatca tgacgctatt aaatgaactg aagcgggaat ttaataccgc
     4981 catcattatg atcacccacg atcttggcgt ggtggcgggg atctgtgaca aagtgctggt
     5041 aatgtacgcc gggcgcacga tggaatatgg caatgcgcgc gatgtctttt atcaacccgt
     5101 tcatccttat tctatcggtt tgctcaacgc ggtgccgcgt cttgatgcgg aaggtgaaac
     5161 aatgttgacc atcccgggta atccgccaaa cttgctgcga ttaccgaaag gttgcccgtt
     5221 ccagccacgt tgtccacatg cgatggaaat ttgtagtagc gcaccgccgc tggaagagtt
     5281 tacgcctggc cgtctgcgtg cttgctttaa accggtggag gaactgttat gaatgctgta
     5341 actgaaggaa gaaaagtcct ccttgaaatc gccgatctta aagtgcactt tgaaatcaaa
     5401 gatggcaaac agtggttctg gcaaccgccg aaaacgctca aagccgtcga tggtgtcact
     5461 cttcgcctgt atgaagggga aacattaggt gtggtagggg aatcgggatg cggtaagtcc
     5521 acctttgctc gcgccatcat cggtttggtc aaggcgaccg acggtcatgt tgcctggtta
     5581 ggtaaagagt tgctgggcat gaagcccgat gaatggcgtg ccgttcgcag tgatattcag
     5641 atgattttcc aggatccgtt ggcatcgcta aacccgcgta tgaccatcgg cgagatcatc
     5701 gctgaaccac tgcgtactta tcatccgaaa atgtcacgcc aggaagttcg cgagcgcgtg
     5761 aaggcgatga tgctgaaagt cgggttattg cctaacctga ttaaccgcta tccgcatgag
     5821 ttctccggtg ggcagtgcca gcgtatcggg attgctcgtg ctcttattct tgaaccgaag
     5881 ctgattatct gcgatgagcc ggtttcggcg ctggacgtgt caattcaggc gcaggtggtc
     5941 aacctgctcc agcagctgca acgtgagatg ggattgtcat taatttttat cgctcatgac
     6001 ctggccgtgg taaaacacat ttccgatcgt gtgttggtga tgtatctcgg ccatgcggta
     6061 gaactgggga cctatgatga ggtctaccac aatccactac atccttacac cagggcattg
     6121 atgtcggcag tccccatacc tgatccggat ctggagaaga acaaaaccat ccagttactg
     6181 gaaggggaat taccgtcgcc gatcaacccg ccttccggtt gtgttttccg tacccgttgc
     6241 ccgattgccg gtccggagtg cgccaaaaca cgtcctgttc tggaggggag tttcagacac
     6301 gccgtttctt gcctgaaagt cgatccgctt taaacacaat aagggctgac agttgtcagc
     6361 cctttttcac gctaaaagcg attatttatt cccgccagat gatatggcaa agtttgtgat
     6421 ctttttcgcg gcataacaga atgcgggcga aaacatcgtt gatttcacca tcttcactgt
     6481 ccgccagacc aatcaccact tcggcaaaaa agtctgggtt caaatcgaaa tcaacatgtt
     6541 cctgccagtc ttccgccgga tcgaataact ccgcgccgcc gcgctcttca aactgaagat
     6601 tgaacagcag aacatcagct ggatcgagat tgtcggcagc cagttcgaga aaaatatcgt
     6661 aggcctgctc aagcgtttca tcttcagtca ggcgattgtt tagatccata tccatagtca
     6721 ctacctgttt aacctctgtt ggcgacgttt tacagcaacg gactgaagaa gtaaaacagt
     6781 cgctcggcga cacgttgcca taatggacgt tttagccata aacgggcatc gagcagacgt
     6841 gaacgcgaaa tataatcgtc ctgaacggcg gcaaggtctg caccaaaacc tttatcgtcg
     6901 attgccaggg taatctcgaa attcagccac agactccgca tatcaaggtt aacggtgcca
     6961 accagactta gttcgccatc gaccagcacg ctcttggtat gcagtaaccc gccttcaaac
     7021 tgataaattt taaccccagc agcaagcagt tccgtaaaga atgcgcgact ggcccagccg
     7081 accagcatcg agtcattttt tcgcggaagg ataatactga catccacccc gcgctgcgcc
     7141 gccgtgcaaa tcgcatgaag taaatcatcg cttggcacaa agtagggcgt ggtcatgatc
     7201 aaatattcac gcgccgaata agccgcagtc aataatgcct ggtgaatgag atcttccgga
     7261 aagccggggc cagaagcaat tgtgtgaatg gtgtgaccgc tggcctgttc aaacggcata
     7321 atattgacat ctggtggtgg cggcagaata cgttttccgg tttcaatctc ccagtcgcag
     7381 gaataaataa tccccatcgc ggtggcgatg gggccttcca tacgcgccat cagatcaatc
     7441 cattgcccta cgcccgcatc ttgtttgaag tagcgaggat cgaccatatt catgctgccg
     7501 gtgtacgcga tgtaattatc gatcatgatc atcttgcgat gttggcgcag gtccatacgg
     7561 cgtaaaaaca cacgcatcag attgaccttt aaggcttcga ccacttcaat accggcatta
     7621 cgcattagct cgggccacgg gctgcggaaa aaagccacac tcccggcgga gtcgagcatc
     7681 aatcggcaat gaataccacg ccgtgcagcc gccattaatg attcagccac ctggtccgcc
     7741 atgccgccgg gctgccagat ataaaacacc atctcaatat tatggcgcgc gagctggatg
     7801 tcgcggatta acgcctgcat cacatcatct gactcggtca tcagttgtag ctgattccct
     7861 ttgaccccag cgatcccctg acgacgctcg caaagcttga ataatggcgc agcgacactg
     7921 ctattttctt cggcgaagat atgcttacag gctttaaggt cgttaagcca ttttgcggtg
     7981 gaaggccaca tcgctctggc gcgctcagcg cggcgtttgc ctaaatggag ctcgccaacg
     8041 gcaagatagg caataattcc gactaacggc agaatgtaaa taatcaacag ccaggccatc
     8101 gcggagggaa ctgcgcgtcg tttcattaga atgcgtaaag ttacgcctgc aatgagcaac
     8161 cagtatccca gaatggccaa ccaactcacc aacgtataaa cggttgtcat agattagaaa
     8221 tccttttgaa agcgcattgt tatgagttta cgcatatgga ttcatctggc aaataaaaac
     8281 gcgggaaaag cactggtcag cgtcgggggt gggggtataa tgaccattct gttattgcat
     8341 agagtagtta acatgaagcg gagtagaacg gaagtggggc gctggcgcat gcagcgtcag
     8401 gctagccgac gtaaatcgcg ttggcttgag gggcaatcgc gccgaaatat gcgtatccac
     8461 agcatcagga agtgcattct aaacaaacag cgtaactcgt tattgtttgc gatctacaat
     8521 atctaaatgt actgggcacc attgcggtgc ccaataagtt tactcttccg tctttaaact
     8581 ctatttttgc gccgattctt tactgtcagc tttcgtttct tgttcatcgt tatcgctgaa
     8641 aataccatga cctgagttca acaacattga aacgagcata tcattattaa tctcttcacc
     8701 atttaaaact cgcgccagaa tttcgctgcc aaacagttgc ggtgagagaa tgatatccgg
     8761 atgtaccatc ttaatcttat tcaggttttt gctatcactg acggcgagaa ctgttttgac
     8821 atcactgctc atatcttttg ccgagagtac aacaaacgcg ttatctgcat cgttatcact
     8881 cagcgccaga atggcccggc aacgatcgat tcccgctttc tttaatactg aactgtcatt
     8941 actgtcaccg gggataacat cagcgttatc gcctaagcgt tgctcaagtt gcttgatatc
     9001 atcttcaggc aagttgctga taaccgttac gttttgtccg cgttgattca gttgcaggat
     9061 cgtattgatg gcgagaatcg aatgtccgca aacaataaaa tgatctttac gatgcattgt
     9121 atgattgttt ccttttacaa gtttgttgaa tcccccgcgg ataagcggac caaaaattga
     9181 ggtcatagat gtggcaaaaa cggtaatgcc ggaaataatg accgaaatag tgaacaatcg
     9241 tgctgattca gaaacaggga caatatcgcc atagccgacg gttgacatgg tttctatcga
     9301 aaaatagaac gcggtcatca aactttctat tcgcggatta aaaccttcgc ttaaataaag
     9361 cgcaccgtag gttgagtaaa acagtaatgt cgtgaaacta ataaatgcaa aaattgtccc
     9421 ggctgcggcg ctactgtggg agaagtcttt gcgcagtatc agcaaaaaca ccagcgtaaa
     9481 aatgcaaaat ccaatactaa atttcagcca gggataaaaa tgcagggtgt aaattagcgc
     9541 tatcaacaac aaaatgatac tgattgccca tgcgagcttt gcccggaaca gtaagccaat
     9601 ggagttcagg acaagaaaga cgccgagcat aaatagtggg gcgtttgcca gcaaggagag
     9661 atccaactct gaaaatgctt tgatatggaa aatatcaagc aaattgaccg acataccata
     9721 gattgtttta aaaataagca atccatttaa aaagacggcc agcgcgagaa tgtcgtgccg
     9781 aagggtcacc cataaatttg ttgctgtttg tttgaatgta gcccagtgac tcactgatac
     9841 ctctgtaacc acaattttgt ttaattatct tagcgtgcac attttctgaa catacatgca
     9901 gcgcgtgaat gtgttaaaca atagtagatg actttatccg cagtaatcaa aggatttgag
     9961 cgagcaactg tacctcagga aaggtcattt ttcctgaata tgctcacatc atataaagaa
    10021 atacagataa agttattatc tgcttgtggt agtgaatgca ctgaccggct ataaggaaag
    10081 gccaaacaag aacacggttg caaaaaccgt gcccttaaat attgaatctc tattcagaac
    10141 actttcttaa atggtttcac tgaaacgtgt tcatagactc ctgccgctac gtacgggtca
    10201 gcatcggccc aggcctgagc tgcttccagc gattcaaatt cagcaataac ggttgagcca
    10261 gtaaatcccg cagcccctgg atcgttactg tctaccgctg gcattggacc agctgtcaac
    10321 aaacgacctt catcatgcag taactgtaaa cgtgctaaat gtgccggacg aacggaaagg
    10381 cgtttttcga ggctatcagc tttgtcttga gcgtaaataa catacaacac gggcacaact
    10441 ccttgttcgg gaaagttgta aagtacgtta tttgaaaggg cgaagatctg caacggaaag
    10501 atgatgtctt tgttaagacc atgcataaat taaggtcaat tacgccaaaa atgacatttt
    10561 cactgatcct gatcgtcttg ccttattgaa tatgattgct atttgcattt aaaatcgaga
    10621 cctggttttt ctactgaaat gattatgact tcaatgaccc ttgatttacc tcgccgcttc
    10681 ccctggccga cgttactttc ggtctgcatt catggtgctg ttgtggcggg tctgctctat
    10741 acctcggtac atcaggttat tgaactacct gcgcctgcgc agccaatttc tgtcacgatg
    10801 gttgcgcctg ctgatctcga accgccacaa gccgttcagc cgccacc
//
LOCUS       DAMZET010000143        10775 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.143, whole genome shotgun sequence.
ACCESSION   DAMZET010000143 DAMZET010000000
VERSION     DAMZET010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.143"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(1..2793)
                     /locus_tag="POB34_003688"
                     /pseudo
     CDS             complement(1..2793)
                     /locus_tag="POB34_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pertactin family autotransporter"
     gene            complement(3634..4725)
                     /gene="ychF"
                     /locus_tag="POB34_003689"
     CDS             complement(3634..4725)
                     /gene="ychF"
                     /locus_tag="POB34_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDI5920561.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(4842..5426)
                     /gene="pth"
                     /locus_tag="POB34_003690"
     CDS             complement(4842..5426)
                     /gene="pth"
                     /locus_tag="POB34_003690"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDI5920562.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            5704..5982
                     /gene="ychH"
                     /locus_tag="POB34_003691"
     CDS             5704..5982
                     /gene="ychH"
                     /locus_tag="POB34_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HDI5920563.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(6037..7716)
                     /gene="dauA"
                     /locus_tag="POB34_003692"
     CDS             complement(6037..7716)
                     /gene="dauA"
                     /locus_tag="POB34_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HDI5920564.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(7841..8788)
                     /gene="prs"
                     /locus_tag="POB34_003693"
     CDS             complement(7841..8788)
                     /gene="prs"
                     /locus_tag="POB34_003693"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDI5920565.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(8939..9790)
                     /gene="ispE"
                     /locus_tag="POB34_003694"
     CDS             complement(8939..9790)
                     /gene="ispE"
                     /locus_tag="POB34_003694"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDI5920566.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGADISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(9790..10413)
                     /gene="lolB"
                     /locus_tag="POB34_003695"
     CDS             complement(9790..10413)
                     /gene="lolB"
                     /locus_tag="POB34_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HDI5920567.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLNEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            10627..>10775
                     /gene="hemA"
                     /locus_tag="POB34_003696"
     CDS             10627..>10775
                     /gene="hemA"
                     /locus_tag="POB34_003696"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="catalyzes the formation of glutamate-1-semialdehyde
                     from glutamyl-tRNA(Glu) and NADPH; the second step of the
                     pathway is catalyzed by glutamate-1-semialdehyde
                     aminomutase which results in the formation of
                     5-aminolevulinic acid; functions in porphyrin
                     (tetrapyrroles) biosynthesis; the crystal structure showed
                     a C-terminal dimerization domain that appears to be absent
                     in Chlamydial proteins; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDI5920568.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLST"
BASE COUNT         2704 a         2735 c         2638 g         2698 t
ORIGIN      
        1 gttatactgt gcactgactc ccacgccgtt attccagcca ttacctttga aactgtactt
       61 ctcccgggag ttgttcaaca gatagtctgt atcccctgaa aactcgcgga tagctccagt
      121 cttcacatac atattcagct ggctgttgcc tgcggtgatg tcatatccca gtatcatgct
      181 ggcacgcccc agcagcgatt cgtagtgatt cagatgtata ttgagaccat tactcgcctt
      241 catagccatc tcattctggt ggctgtatgt gagctgggtt tgcggctcta tatagaaccc
      301 ataaccagta ggggtcaggt tgaacctctg cccggcttcc agggagatgc tcaatccatt
      361 cgcagtgccg ttggcgttaa cgccgttgtt ctgactgtcc agtacgtgaa aactattttt
      421 ctggcgcgat gcttttacaa cgagatcgct gtaaaaaccg ttgtgtgcca tgtaactggc
      481 atacattccc atgtagtctg aacgtgcggt accgtcgcct ccgctatagt ccggcgatgc
      541 atgtgttgag ccaatataca gaccgacata caacggcatt acatcagaga gacgtttatc
      601 cccaccaaac tggatactgc tgtaacccat gtcaaagccg ctcagtttgc cactggcgaa
      661 ggagtccagg cttcccccat aactgcgcag ccagatatta ccgtctttac tctgatttcg
      721 caggtcaccc attcgttgca tcagcgtacg gttttcaaca tagtttaata agtaaccgac
      781 attgagataa ttgccgccag catctgcggt cgtggtgggt ttaggtgtgg gagtggggtt
      841 aggcgtagga tcgggttcag gcgtaggatc ggggttgact atgggaggct gagcgggagc
      901 cggtgttggt tcaggattag gagtcggttc cggaactgtc ccggaagcgt aaagctccca
      961 gttagtgcca tttttacgca catcgtacag atatcccccc aactcaacct gagaagacgc
     1021 gctgaacgag gccgcaccgt cagtggtttt taccactgtc agaacttcat ttcccgtagt
     1081 ggcctcgctg ccctggttgc ggatagccaa tacgtgatta ccagcactac ttccgctgat
     1141 attcaataaa tcccctttat tattaacgcc attaccctcg ccaacaacat cagcacgcat
     1201 aataaaggta ctgttaccgc tcaggttctc tacgtttaat gtggcaaatg tgccggcagt
     1261 tgacccgtgg ctggcaaaat cgacagttga atggctcagc gccagcgtgt cgagattaga
     1321 attacttgtt acgttccaga cgctgttatt cattgcaacg tccagtttcc cgccattgac
     1381 attatcgctg agggaggaac ctgtccaaac cgaccctggg tgcatatcta gattgattag
     1441 cccacctttg gaaagaacgc taccgttgat aagcatacga cccgaggcgt tgatgcggct
     1501 ggcggcataa ccatcgtcat gttacgttgc aatggccatc tggtcgggtg tactcatatc
     1561 aatgaccaga tcgctcgtga ggtcgatctg gctgttggtc atggcataaa ttcccctggc
     1621 tcctgccgcg ccggtgatag ccaaactgtc tccggttatt ctaccgccgc tgagcgccca
     1681 caaacccagc gccagactgc cattacgatc aactgtaata tcggtatttt gcatgttgac
     1741 aacagccgtt gccgtctggg ccgaggcacc ataagaaccg ccggaaaaga tgctgtttcg
     1801 ttgcgctgcc gtgccagaaa aattgattgt cgcgtctgaa ccactggtga cgaggccacc
     1861 gccctgcgcg gaagaaatat ggctatctgc accgatagtg gttgtaccac cacgaacttc
     1921 gacgccattg gccgcggctc cagttacatc aacagtgagt gcattcgcgc taacctggcc
     1981 aaagctccag aggccgtgtg cattatcgcc attggtttta atggaactgt ttgttccgag
     2041 atcgacaaca gagtttttct gtacgtttat ccccatggcg ctgtagccct gaacatcgat
     2101 tgtcaggtct gtagccgtaa aacgcgcagc accattggcg ttattgccgt tgagaccacc
     2161 gatataaaca cctgtacttc catcggtcgt gattttactt ccgcttccaa gatcgatact
     2221 ggtgccatag tcattgatgg ttaggcctat accgttcgag ttttcaatgg tgaattgagt
     2281 ggctgttagt gttgagctat gcccaataat gatgccgtca tcgttactct taatggtgct
     2341 gcctgtgcct aagtcagcat gggtatagtc accaattaag ttaatgccga tggcagaggt
     2401 ttgaccaaca acatctactg tgagtcggtt agctgtcagg gagctgtttt tgccctgaat
     2461 aatgattcct tttgcatatc cagaggcgtc gttgacattg acggtgacat cattacccag
     2521 gttgatattg ccaggcgtgt ttcctgctgg ggtcattaca ccatagaggt ttcctgatgg
     2581 atcggcggta tctcctgtaa ttctgtcacc ttcattaata gtaatattgg tgccatattg
     2641 acttcctgta tcagcagcta tcagcagggg gggaaagata attgcattca aaccaatagc
     2701 gccaaatttt atcagttgaa ttgagggcga acggattttt aattcagcga gacgatctgc
     2761 tttggtattc ccattgtgtt gtttgatgcc cataccactc ctatatagta cccagactat
     2821 gggtctatgg attttgtcat tttgtttaaa tcaatgaata atatcctctt atcatcatga
     2881 tatttgtatg gtgcaatcat gatgagcttc tcagagaata attctctatt accgggcaaa
     2941 atcaggaaca caggaattaa tatatattta acatccccct tacaaaggtg agtttatatc
     3001 gagttattcc ctaagttgtc gttgcttatt ccaattcgtt actataaata tggcaaaaat
     3061 attacaacaa acagcggtta aaaaatttgg tgaagatttt ttgtaataat cctaattagt
     3121 tatgggaatt tcgcttgatg tatctgcata acaaaaacaa tatgcgccat ttttgcatta
     3181 ctaataagaa aaagcaaaaa acatcgatat tcatttttgg tcaatgggtt ttctggccta
     3241 tcgttgttat tgtgctactg gttatcattt tcatcttgcc aggatcgcac gtaatcacgt
     3301 tattcgggga ctttatctgc gggatattac ccttcaggta agtccttttc tcgccagcat
     3361 attggagcgt gatcaatatt gatcagctgt gaagggggtg atgtgtagtg gaccaaaact
     3421 tacgttatat cactactaat gagatatccg tcatctgaca ctgatgtctt taattattct
     3481 cgtggttcac tgtaggcaat aagcacaaaa gtgtaggatg ttacaagaat gattaggact
     3541 cggtgaaatg aaaaatccac gcaattgcgt ggattatata tacttttgcg ctcttcatga
     3601 gatttagcga aacctcatga gacaacaaat taattagacg ttgaaaagga agttcatcac
     3661 atcgccatct ttaacgatgt aatctttacc ttctgcacgc attttgcctg cttctttcgc
     3721 gccttgttca cctttgtaag tgatgaaatc ttcaaacgag atggtttgtg cacggataaa
     3781 gcctttttca aaatcagtat ggattttgcc cgctgcctgc ggcgcggttg ctccaaccgg
     3841 aatggtccat gcacgcactt ctttcacccc agcggtgaag taagtttgca ggttcagcag
     3901 cttataaccg gcacggatca cacggttcag gcccggctct tccagcccaa gctcctgcat
     3961 aaactcgtca cgttcttcgt cgtccagttc ggcaatgtct gcttcaacag cagcacaaac
     4021 cggaaccaca acagaacctt ctttcgccgc gatttcacgc acctggtcaa gatatgggtt
     4081 gttttcaaaa ccgtcttcgt tgacgttggc gatgtacatt gttggtttca gcgtcaggaa
     4141 gctcaggtaa cgaatagccg ctttctcttc agcgcttaaa tccagcgcgc gcagcatacc
     4201 tgcattttcc aactggggca ggcatttttc caggaccgcc agctcagctt tcgcgtcttt
     4261 atcgccacct ttggctttct tctgtacgcg atgaatcgca cgttcgcagg tgtcgaggtc
     4321 tgccagcgcc agttcggtgt tgataacttc aatatcgtca gccgggttaa ctttgccgga
     4381 aacgtgaatg atgttgtcat tttcaaagca gcgaacaacg tgaccgatcg cttcggtttc
     4441 acggatgttg gtcaggaact ggttacccag accttcgcct ttcgatgcgc cttttaccag
     4501 accggcgata tcgacaaatt ccatggtcgt gggaagcgta cgttgcggtt ttacgatttc
     4561 agccagttga tccaggcgag ggtcaggcat tggtacgacg cctgtgttcg gctcaatggt
     4621 gcagaatgga aagttggccg cttcaatacc ggctttggtc agcgcgttga acagggtaga
     4681 tttcccgacg ttgggcaaac cgacgatacc gcatttgaat cccatgattt aaatcacctt
     4741 aatatcttaa taatcaacct gttattgcta acagattgca gaaatggaaa taactttgcc
     4801 tattatacac ggcactcggc aaaaatgccg cagacaacga cttattgcgc tttaaaggcg
     4861 tgcaatcggt tcgttgcttt ggtcaagcca tctgtaaacc acatttcagt acaacgcgcc
     4921 gcttcgtcga tggcttcatc aattaacttc tgttcactaa caggcggttt gcctaacaca
     4981 aaaccgacaa ctttattttt atcgcccgga tgaccgattc cgatgcgtaa acggtgaaag
     5041 ttagggttat tacccaattt actgatgatg tctttcagtc cattgtgacc accatggcca
     5101 ccgcccaatt taaatttggc gacgccagga ggcagatcca gttcgtcgtg ggccaccaga
     5161 atttcgtccg gattaatgcg gaaaaaactg gccatcgccg caacggcttt gccgctgaga
     5221 ttcataaatg tagtcgggac taacaggcgg acatcttcgc ctccaagagt gactcgcgaa
     5281 gtataaccaa agaatttagc ctcttcgcgc agcggagcgc gcaaacgctc tgccagtaag
     5341 tcaacgaacc aggcaccagc attatgtcgc gttgcggcgt attcagcacc ggggttcgcc
     5401 aggccgacaa tcaatttaat cgtcacgttt ttttgtcctg agtgtgtaca taactggcgc
     5461 gtagtttact ggttgcggcc ccgcttgaca aaaaactgcg tatcaaatgc agataacgta
     5521 ataattgcat gagtggacta ttagaaagtc aaggtgttca ggcgtttatt tgtaaagttt
     5581 tgttgaaata agggttgtaa ttgtgatcac gcccgcacat aacccacagg gtgttgtcta
     5641 tactttacac ataaggaaga ggggtattcc ctgttacaac ccagaaagtt ccggaggtga
     5701 catatgaaac gcaaaaacgc ttcgttactc ggtaacgtgc tcatggggtt gggtctggtg
     5761 gtaatggtgg tcggcgtggg gtattcaatc ctcaaccagt taccacagtt taatatgccc
     5821 cagtatttcg cacatggtgc agtgctaagt attttcgtcg gtgccattct ctggctggcg
     5881 ggtgcccgtg ttggcgggca tgaacaggtg tgcgaccgtt actggtgggt tcgccactat
     5941 gacaaacgtt gccgccgtag cgataatcgc cgtcatagct aacaaataat gcagtttgct
     6001 gaccagtcag tttgcgctga ctggtcaatc tcgtacttat aaatccgcca tcgccgcgcg
     6061 acgattcggg aagaacgcca ggcgtcccgg gatcggttga atgccagcgc gagccatagt
     6121 gcgcagtggc tggaattcca cgttgcacac gcgcagttca catccctcgg gcagacgctt
     6181 cacaaaacgc tggaacgcat caagaccacc agcatcaagt accggaacgg catcccactt
     6241 cagaatcaca atccgtttgc cttcaagacg tgactccagg tccgtgaaca agccttcagc
     6301 agcagcaaaa aacagcgggc caataacacg cagaaccagg acatcgtctg gaacatctac
     6361 gactaccggt gccaggcgag tcatacgtgc gatacgacgc ataaacagca gcgatgccag
     6421 cacgatcccc acgctgatgg caataaccat atcaaacagc acggtcaacg acatgcacag
     6481 cagcatgaca atgatgtcat ctttcggcgc atgacgcagc aagtcgacca ctttgtgcgc
     6541 ttcactcatg ttccacgcca ccatcaacag cagggctgcc atggcggaaa gcggcagcca
     6601 ggagagcagc ggtgccagta ccagcagggc aagaataacc agaatagagt ggatcaccgc
     6661 cgagataggg gaagttgccc cggcacggac gttagcggca gaacgcgcga tggcagctgt
     6721 agcggtaata ccaccaaaga acggagcgat aatattcccc agtccctgtc caaccagttc
     6781 gctgtttgcc ttgtgttttg tcccggtcat accatccagc accacggcgc agagcagaga
     6841 ttcgattgcg ccgagcattg ccattgagaa tgccgcaggc agcagtgtgc gaatagaatc
     6901 ccaggtaagc gtgaattctg aattaggcag atcccacggc agcaccagtt gcggcagcag
     6961 ttgcggaata ccgttaccct gagaaccatc ggccaggacg tagtggaatt gcgaaccgat
     7021 ggtagcaaca tgtccgccga gcaggttaac aatccccatc accgcgcaac cagccagcaa
     7081 ggccggaagg tgaccgggta aacgaatgcc cagacgcggc cagaaaacca gaatgccaag
     7141 cgtcacaatg ccaatggcag catcacccac attaatggtc ggcagcgcca taaataatgc
     7201 gccgactttt tgtagataat gttccgggac atgggccatt tgcagaccga gaaaatcttt
     7261 aatctgcatg gtaccgatgg tgatcccgat acccgaggtg aaacctaagg tgacggaaac
     7321 cggaatatac tcaatcaggc gaccaaagcg tgccagaccc atcagaatca aaaagatccc
     7381 cgacagcaag gtcgcaacca gcagtcctgc cagtccaaac tgttgcgaca cgggatagag
     7441 aattaccaca aatgccgcag tcggaccgga aacgctaaag cgtgatccac ccgtcagagc
     7501 aatgacaatc cccgcaacag ctgcagtata taaaccgtac tggggtgcca caccactacc
     7561 aatagccaac gccatcgcca gcgggatagc aataatcccg acggttatcc cggcaatcag
     7621 gtcacgggta aaccgtgcgg cagtatattt ttctttccag caagcgtcga tcagagcgcg
     7681 gaaaggcatc acatgtgagg aaaatatttt gttcacaata atgtttcatc cgtgagcgca
     7741 tcatctgtca actaaatggc aggtgaagga ggcataggtc atacaaatgg atattacaga
     7801 caaaaaaacc cgccgcagcg ggtctttgag ccgggttcga ttagtgttcg aacatggcag
     7861 agatcgattc ttcgttgctg atacgacgaa tcgcttcggc cagcatacct gacagggtca
     7921 gagtacgcac gttcggcagt gatttgattt catcgctcag cggaatggta tcgcagacaa
     7981 cgacttcatc aattacagag ttacgcaggt tgttcgccgc gttgccagag aagatcgggt
     8041 gagtcgcgta cgcaaataca cgtttagcac cacgttcttt cagagcttca gcagctttac
     8101 acagcgtacc gccagtgtcg atcatatcat cgaccagtac gcagtcacga cctgcaacgt
     8161 caccgatgat atgcatcacc tgggaaacgt tcgcacgcgg acgacgtttg tcgatgattg
     8221 ccatatcggt atcgttcagc agcttagcga tagcgcgggc acgcacaacg ccgccgatgt
     8281 ccggagaaac cacaattggg ttatccagat tcagctgcag catgtcttcc agcaggatcg
     8341 ggctaccaaa tacgttatca accggaacgt cgaagaaacc ctgaatctgt tcagcgtgca
     8401 gatccactgt cagcacacgg tcaacaccga cgctggagag gaagtctgca accactttcg
     8461 cagtgattgg tacacgagcg gaacggacgc gacggtcctg gcgcgcatag ccaaagtagg
     8521 ggataacagc ggtgatacga cctgcggaag cacgacgcag ggcatcaacc ataacgacta
     8581 attccatcag gttgtcgtta gtaggggcac aagtggactg gatgatgaaa atatcaccac
     8641 cgcgtacatt ttcattaatt tgtacgctga cttcgccgtc gctaaagcga cctacagcgg
     8701 cgtcgccgag tgaagtgtac aggcggttgg caatacgttg tgctagttcc ggggtggcgt
     8761 taccagcaaa aagcttcata tcaggcacga gaagaacctc aggcatgcgt ccattggtgg
     8821 aaagaatctg ccgaaaactg tgcgggccag gcatgatcct ttccaggcgg tgtatttaag
     8881 agcgcgatgc aacgtctgga acaaggtgac gttgtcaccg aaactcagct tgcccggctt
     8941 aaagcatggc tctgtgcaat ggggaaagat taacgccttt cgccacaaag ccattgagcc
     9001 attccggggc ttgctctagc acctggcggg cttcagactc tgtatcaaat tcagcaaaga
     9061 cacaggcccc tgtcccagtc aggcgcgacg gggcgtattc taacagccag gaaagcaccg
     9121 catcaacctc gcgaaaacgt tttcttgcga taacctcgca atcattgctg aattcacatt
     9181 ttagcaacgt ttctattgac ctttttggcg tattgcgcgg gagttcagga tctttaaaaa
     9241 tcaccggagt cggaatactt acaccagggt gcgccaccag ataccacttc tctggcggat
     9301 ccaccggcgt tagtatttca ccaacgcctt cggcaaacgc ggcatgcccc cgaacaaaga
     9361 caggaacatc tgcgcccagc gtcagcccca tttccgccag ctcatccatg cttagcccgc
     9421 attgccagag atgatttaat gccaccagga ccgtcgcggc attggatgaa ccaccgccga
     9481 gaccgccgcc cattggcaaa cgcttgtcaa tgctgatatc cgcaccgctt cccgtcggaa
     9541 gacgcccgct gtctgccgca gttttcatca acaaccgcgc tgcgcgaacg atcaggttat
     9601 cttcatgttc cacgccttca acgggcgtta acagacgaat atccccatcg tcacgaagct
     9661 caatgctaat ggtgtcgccg taatcaagaa actgaaacag cgtttgcagc gtgtggtaac
     9721 catccgcacg ctgaccggta atgtataaaa acagattaag ttttgccgga gagggccact
     9781 gtgtccgcat tatttcacta tccagttatc catttttaac ttgatgcgtt gaccaccgtc
     9841 ggtgagttcc atattggctg gcatcgcagg ttgcgttttg gtgtcataac caccgtaaac
     9901 aactttccag tttttgccat tctggctgta ggtaatttcg ttcaggcggt actggtcgtc
     9961 cagtttgtag tcggttgcat cacccggtaa acctaaaatc cactggcgca agctgttgag
    10021 cggaatcggc attccggtca atttgccaat catctcttcg gcgtcatcgg cggtataacg
    10081 ctgaccttta ttgtcgacta actgcacgtt acccggttga gcattcagct caagttccgt
    10141 actgcccaat gggttagtca gcagcagacg gtagcgatcc tgaccagttt gctgccagaa
    10201 gaaacgggcg tacacttttt gttggtcaga aatataagcg aacgcgccgc gagtttgata
    10261 ctgattaaga ttgcgcacgt cttgctgatg ctgacgccat tgtggcgaat ccgggctttt
    10321 accaggacct ttgggcgtgg taacggaaca ggcagtgagc acaagagcag ccagcggtag
    10381 caggcggata agacgaaaat cgggcagggg catagtgatg acaagtcctt gagatacgtt
    10441 gcagttataa cccttaatgc tagcgttacc gtccgctatc gtctatgttc aagttgtctt
    10501 aattgccaga atctaacggc tttcggcaat tactccaaaa gggggcgctc tcttttattg
    10561 atcttacgca tcctgtatga tgcaagcaga ctaaccctat caacgttggt attatttccc
    10621 gcagacatga cccttttagc actcggtatc aaccataaaa cggcacctgt atcgctgcga
    10681 gaacgtgtat cgttttcgcc ggataagctc gatcaggcgc ttgacagcct gcttgcgcag
    10741 ccgatggtgc agggcggcgt ggtgctgtcg acgtg
//
LOCUS       DAMZET010000144        10687 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.144, whole genome shotgun sequence.
ACCESSION   DAMZET010000144 DAMZET010000000
VERSION     DAMZET010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.144"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(34..118)
                     /locus_tag="POB34_003697"
     tRNA            complement(34..118)
                     /locus_tag="POB34_003697"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(82..84),aa:Tyr,seq:gta)
     gene            complement(153..237)
                     /locus_tag="POB34_003698"
     tRNA            complement(153..237)
                     /locus_tag="POB34_003698"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(201..203),aa:Tyr,seq:gta)
     gene            complement(397..1239)
                     /gene="purU"
                     /locus_tag="POB34_003699"
     CDS             complement(397..1239)
                     /gene="purU"
                     /locus_tag="POB34_003699"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HDI5920569.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(1289..1747)
                     /locus_tag="POB34_003700"
     CDS             complement(1289..1747)
                     /locus_tag="POB34_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HDI5920570.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            1860..2765
                     /gene="rssA"
                     /locus_tag="POB34_003701"
     CDS             1860..2765
                     /gene="rssA"
                     /locus_tag="POB34_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HDI5920571.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            2857..3870
                     /gene="rssB"
                     /locus_tag="POB34_003702"
     CDS             2857..3870
                     /gene="rssB"
                     /locus_tag="POB34_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HDI5920572.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            4072..4980
                     /gene="galU"
                     /locus_tag="POB34_003703"
     CDS             4072..4980
                     /gene="galU"
                     /locus_tag="POB34_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDI5920573.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(5124..5537)
                     /gene="hns"
                     /locus_tag="POB34_003704"
     CDS             complement(5124..5537)
                     /gene="hns"
                     /locus_tag="POB34_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HDI5920574.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            6141..6758
                     /gene="tdk"
                     /locus_tag="POB34_003705"
     CDS             6141..6758
                     /gene="tdk"
                     /locus_tag="POB34_003705"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDI5920575.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFAEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRNHYKEALQVGSLTAIQERHRHD"
     gene            complement(7060..9735)
                     /gene="adhE"
                     /locus_tag="POB34_003706"
                     /gene_synonym="adhC"
     CDS             complement(7060..9735)
                     /gene="adhE"
                     /locus_tag="POB34_003706"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDI5920576.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            10212..>10687
                     /gene="ychE"
                     /locus_tag="POB34_003707"
     CDS             10212..>10687
                     /gene="ychE"
                     /locus_tag="POB34_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HDI5920577.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGILPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIA"
BASE COUNT         2747 a         2401 c         2643 g         2896 t
ORIGIN      
        1 ttcgtttgag agttcaggga cttttgaaag tgatggtggt gggggaagga ttcgaacctt
       61 cgaagtcgat gacggcagat ttacagtctg ctccctttgg ccgctcggga accccaccag
      121 gggtaattca aattttgagg taatgcttga gatggtggtg ggggaaggat tcgaaccttc
      181 gaagtcgatg acggcagatt tacagtctgc tccctttggc cgctcgggaa ccccaccacg
      241 gggtaatgct ttttactggc ctgctccctt atcgggaagc ggggcgcatc atatcaaatg
      301 acgcgccgct gtaaagtgtt acgttgagaa aaatgaactg gttgcgtaat tttcatccgt
      361 aacggattaa aggtaaccag ttatttttgc tggcgattaa agaataatcg tccgattacc
      421 gtaaacaaag acgcgctgtg ccagtacttt gtatagcgca cgacttaaga cgtttttctc
      481 gacgtcacga cctgcgcgca tcatatcttc agctgtgtag gtatgatcga catgaataac
      541 gtcctgcatg atgattgggc cttcgtccag attgtcattc acatagtgag cggttgcgcc
      601 aataatcttc acaccacgtt cataggcctg gtgataagga cgtgcgccga taaacgcagg
      661 caggaatgaa tggtgaatat tgatgatttt attcgggaag cgtgccacaa attccggcgt
      721 taatacccgc atatacttcg ccagcaccac atagtcaggt tgataagcat caatggcgtc
      781 cgccatcttt tgatcgtgct cgttgcggct taacccttca tggcttacca gctcaaacgg
      841 aatatcaaaa cgctcaacca gagaacgtaa agtatcgtgg ttaccaataa ctgccgcgat
      901 ttcgacatcc aggccgccgt aattggcttt catcaacaaa tcgccaaggc aatgcgcttc
      961 tttagtgacc agaatcacta tccggcgacg accggcagga ttcagctcac gcacggagcc
     1021 ttctggcaat gcgctatcga gatccgccag aagggtggaa tcattaaaaa tcccttccag
     1081 ttccgtgcgc ataaaaaagc gcccggtacg gtgatcaaca aattcattgt tctgtacgat
     1141 atttaactcg tgcttgtagc aaatattggt aatacgtgcg atcagacctt tttggtccgg
     1201 acaaatagta cgcagaactt tacgttggag tgaatgcatt gctggaaaaa ccttgttgag
     1261 agtgtttgct aaaccgtacc gtcaaccatt attggccgca gcacttttta aattttttac
     1321 ctgaaccaca agggcaggga tcgttgcgac caaactgcgg acgtgtaccg tcaatatagt
     1381 accattgacc gttttctttc aaaaatcgcg aacgttcaat tattgcacct gttttgccac
     1441 cttcggtaaa tcgagcaaca aaactgacaa atccgatatt gtcggcgtcc tgccaacaat
     1501 gttcaaatac ggtcaatccc aaccattcag tatgcgcaaa tccggccatc aattcggcac
     1561 gtaacgccgc tgctccacaa gagggatgcc aggtctttat taaataatct gcgtcttgca
     1621 tcacaaaagc gcagtaacgc gaacgcatga gatgttctgg atcaggtgca accttttcac
     1681 cagacacata agggtggcaa cataggctat actcgacagc actaccacag ggacaaagct
     1741 gagacacaaa taatctccct ggaaacaata acggcgtatt aaccgcctga gtagcactat
     1801 gttaaccgag cagtagcgat gtggctacga ttgcattcca ggggaatctt gcgggaataa
     1861 tgagaaagat aaaaataggg ctggcgctgg gatctggcgc ggcgagaggt tggtcgcata
     1921 ttggcgttat taatgcgcta aaaaaagtgg gtattgaaat tgatatcgtt gcaggatgtt
     1981 caattggttc gctggtgggc gcggcctatg catgcgatcg attatctgcg ctggaagatt
     2041 gggtaacctc tttcagttat tgggacgttt tacgcctgat ggatctctcc tggcagcgcg
     2101 gtgggttact gcgcggcgag cgtgtcttca atcaataccg cgaaataatg ccggaaacag
     2161 agatcgaaaa ttgttcccgt cgctttgcgg ctgttgccac caatttaagt acgggacgtg
     2221 aattatggtt tactgaaggc gatctccatc ttgctattcg cgcatcatgc agtattccag
     2281 gactcatggc acctgttgca cataacggct actggctggt tgatggagca gtcgttaacc
     2341 caattcctat ttcactcacg cgtgcattgg gtgctgatat tgtgatagcg gtcgacctgc
     2401 agcacgatgc tcatttgatg caacaagatt tgctctcctt taatgtcagt gaagaaaata
     2461 gcgagaatgg tgattctctg ccgtggcatg cgcgtctgaa agaaaggtta ggcagcataa
     2521 cgacacgtcg ggcggtgaca gcaccaacgg caacagagat tatgaccact tctatccagg
     2581 tgctggagaa ccgccttaaa aggaaccgca tggcaggtga tccgcccgat attctgattc
     2641 aacctgtttg cccgcaaata tctacgcttg atttccatcg cgcgcacgct gccattgcgg
     2701 ccggacagct ggcagtggaa aagaaaatgg acgaactttt gccgttggta cgcaccaaca
     2761 tttgaccaga atttttatct acacttaagt taattctgac aggcgcaggt ggcaatagca
     2821 tgccactatt gagtaaagcc agtcagggga gagaacatga cgcagccatt ggtcggaaaa
     2881 cagattctca ttgttgaaga tgagcaggta tttcgctcgc ttctggattc gtggttttcc
     2941 tcattgggag cgacaacggt actggcggct gatggggtgg atgcccttga gttgctggga
     3001 ggtttcactc cagacctgat gatatgtgat atcgcgatgc cacgaatgaa cgggcttaaa
     3061 ctgctggagc atatacgtaa cagaggcgac cagaccccag ttctggtgat atctgccact
     3121 gaaaatatgg cagatattgc caaagcgtta cgtctgggcg ttgaagatgt tttgctgaaa
     3181 ccagttaaag atctgaatcg cttgcgcgag atggtttttg cctgtctcta tcccagcatg
     3241 tttaattcgc gcgttgagga agaggaaagg ctttttcgcg actgggatgc aatggttgat
     3301 aaccctgccg cagcggcgaa attattacag gaactacaac cgccggttca gcaggtgatt
     3361 tcccattgcc gggttaatta tcgtcaattg gttgccgcgg acaaacccgg cctggtgctt
     3421 gatattgccg cactttcgga aaacgatctg gcattttatt gccttgatgt aacccgagct
     3481 ggacataatg gcgtacttgc tgccttgtta ttacgcgcat tgtttaacgg attattacag
     3541 gaacagcttg cacaccaaaa tcaacggttg ccagagttgg gcgcgttatt gaagcaggta
     3601 aaccatttac tccgtcaggc caatctgccg gggcagtttc cgctattagt tggctattat
     3661 catcgcgaac tgaaaaatct cattctggtt tctgcgggtc tgaatgcgac gttaaatacc
     3721 ggcgaacacc aggtgcaaat cagtaatggt gttccgttag gcactttagg taacgcttat
     3781 ttgaatcaat tgagccagcg atgcgatgcc tggcaatgcc aaatatgggg aaccggtggt
     3841 cgactgcgct tgatgttgtc tgcagaatga gcaaacgata acgcgggcta aatttgcatt
     3901 acctgctaat gtcggctggt ggtactatcg tcgccattcg tataagtaat tgtcttaatt
     3961 atgctaactc gcctcctttt cagaacttag ccccttcggg gtgctgatat actgggatgc
     4021 gatacagaaa tatgaacacg ttcaaaacac gaacagtcca ggagaattta aatggctgcc
     4081 attaatacga aagtcaaaaa agccgttatc cccgttgcgg gattaggaac caggatgttg
     4141 ccggcgacga aagccatccc gaaagagatg ctgccacttg tcgataagcc attaattcaa
     4201 tacgtcgtga atgaatgtat tgcggctggc attactgaaa ttgtgctggt tacacactca
     4261 tctaaaaact ctattgaaaa ccactttgat accagttttg aactggaagc aatgctggaa
     4321 aaacgtgtaa aacgtcaact gcttgatgaa gtgcagtcta tttgtccgcc gcacgtgact
     4381 attatgcaag ttcgtcaggg gctggcgaaa ggcctgggac acgcggtatt gtgtgctcac
     4441 ccggtagtgg gtgatgaacc tgtggctgtt attttgcctg acgttattct ggatgaatat
     4501 gaatccgatt tgtcacagga taacctggca gagatgatcc gccgctttga tgaaacgggt
     4561 catagccaga tcatggttga accggttgct gatgtgaccg catatggcgt tgtggattgc
     4621 aaaggcgttg aattagcgcc gggtgaaagc gtaccgatgg ttggcgtggt tgaaaaacca
     4681 aaagcggatg ttgcgccgtc taatctcgct attgtgggtc gttacgtact tagcgcggat
     4741 atttggccgt tgctggcaaa aacccctccc ggagctggtg atgaaattca gctcaccgac
     4801 gcaattgata tgctgatcga aaaagaaacg gtggaagcct atcatatgaa agggaagagc
     4861 catgactgcg gtaataaatt aggttacatg caggccttcg ttgaatacgg tattcgtcat
     4921 aacacccttg gcacggaatt taaagcctgg cttgaagaag agatgggcat taagaagtaa
     4981 catccgtatc ggtgttatcc acgaaacggc gttgagcaat cgacgccgtt tttttatagc
     5041 ttattcttat taaattgtct taaatcggac aataaaaaat cccgccgatg gcgggatttt
     5101 aagcaagtac aatctacaaa agattattgc ttgatcagga aatcgtcgag ggatttacct
     5161 tgctcatcca ttgctttttt gattacagct ggagtacggc cctggccagt ccaggtttta
     5221 gtttcgccgt tttcgtcaac gtagctatat tttgccggac gctgagcacg tttagctttg
     5281 gtgccagatt taacggcagc aaggctattc agcagttcgt tcgggtcaat accgtcagcg
     5341 atcagcattt cgcgatattg ctgcagttta cgagtgcgct cttcaacttc agcagcagcc
     5401 gcgctttctt cttcgcgacg ttcgttaaca acaacttcta atttttccag catttcttcc
     5461 agcgtttcaa gtgtacattc tcttgcctgc gcacgaagag tacggatgtt gttcagaatt
     5521 ttaagtgctt cgctcattgt agtaatctca aacttatatt ggggtggttt gttgaggtaa
     5581 taatagagcc ttaaattcag ttgtgcaata gccaggaatg taaggaattc aaaattgttc
     5641 tttattttgt gccgccaata aatatctttt cataaaatta gccagaaaag acgcggcata
     5701 tagccctatt tgcaccgatg atttcgcagc tcgtgaggtt aaaacttcct gattcatgtc
     5761 acattttatg gggagattat cgtaggctga cgacctttca gtcttctgta ttagtcgtgt
     5821 ttacgagaat tccctattaa gcgaatgatg aaaagtagaa cagtcgcaat aagagcaggg
     5881 acttagtatt gcactatctc ctggaggtca acagagggct attacttgcg caacaggtta
     5941 aagattgtga atagttacca gcagtcattt acccgcttat aacaagcgag gcaatagtaa
     6001 tgatagctca gaaggattat gcaaggcttc gtaagggaga acgcatatac cacttctgtg
     6061 catactgttg agctgaaaaa ctgacgaatt atgataaact ccagccaacg ttatttcata
     6121 tcattgaggg cctgtggctg atggcacagc tatatttcta ctattccgca atgaatgcgg
     6181 gtaagtctac agcattgttg caatcttcat acaattacca ggaacgcggc atgcgcactg
     6241 tcgtatatac ggcagaaatt gatgatcgct ttggtgccgg gaaagtcagc tcgcgtatag
     6301 gtttgtcatc gcctgcaaaa ttatttaacc aaaattcatc attatttgct gagattcgtg
     6361 cggaacatga acagcaggca attcattgcg tactggttga tgaatgccag tttttaacca
     6421 gacaacaagt atatgaatta tcggaggttg tagatcaact cgatataccc gtactttgtt
     6481 atggtttacg taccgatttt cgaggtgaat tatttattgg tagccaatac ttactggcat
     6541 ggtccgacaa actggttgaa ttaaaaacca tctgtttttg tggccgtaaa gcaagcatgg
     6601 tgctgcgtct tgatcaagca ggcagacctt ataacgaagg tgagcaggtg gttattggtg
     6661 gtaatgaacg ctacgtttct gtatgccgta atcactataa agaggcgtta caagtcggct
     6721 cattaacggc tattcaggaa aggcatcgcc acgattaata agaatttctt tactgacagg
     6781 gtgagcaggg cacttttatc ctgtcagttc gttttacgca cttcttccgg gctatacacc
     6841 cttctcggca gttttttaac gccgctatac gcctcacagg gctcttaagc accgacgttg
     6901 acttgtgacc tgtaaagtac aatatccctg tgtttacgcg ttatacatcg tcgcaaatat
     6961 gatgaaggct aatgctgtcg gtttatggaa aagttgcttt gggtaaacaa aaaaacggcc
     7021 ccagaagggg ccgtttatgt tgccagacag cgctactgat taagcggatt ttttcgcttt
     7081 tttctcagct ttagccggag cggcttcttt tttcgctgca gtttcacctt ctacataatc
     7141 acgaccgtag taggtatcca gcagaatctg tttcagctcg gagatcagcg ggtaacgcgg
     7201 gttagcgccg gtgcactggt catcgaatgc atcttcagac agtttatcca cgttcgccag
     7261 gaagtctgct tcctgaacgc cagcttcacg gatagatttc ggaataccca gttcagcctt
     7321 cagcgtttcc agccatgcca gcagtttctc gatcttagca gcagtacggt cgcccggtgc
     7381 gctcagaccc aagtggtcgg caatttcagc ataacgacgg cgagcctgcg gacggtcata
     7441 ctggctgaat gcagtctgct tggtcgggtt gtcgttcgca ttgtagcgaa taacgttaca
     7501 aatcagcagg gcgtttgcca gaccgtgcgg aatatggaac tgggaaccca gtttgtgcgc
     7561 cattgagtga catacaccca ggaaggcgtt cgcaaacgcg atacccgcga tagtcgctgc
     7621 actgtgaaca cgttcacgcg ctaccggatt tttagaccct tcgtggtagg acgctggcag
     7681 atattctttc agcagtttca gtgcctgcag agcctgacca tcagagaact cagatgccag
     7741 tacagaaaca taagcttcca tggcgtgagt tactgcgtcc agaccaccga aagcacacag
     7801 ggacttcggc atgtccataa ccaggttggc gtcgacaatc gccatatccg gagtcagcgc
     7861 atagtctgcc agcggatatt tctgaccagt agcgtcgtca gttacaaccg caaacggagt
     7921 gacttcagaa cctgtaccag aagtggtggt gacagcgatc attttcgctt tcacgcccat
     7981 tttcgggaac ttgtagatac gtttacggat atccataaag cgcagcgcca gctcttcgaa
     8041 gtgagtttcc ggatgttcgt acataaccca catgatcttc gcggcgtcca tcggggaacc
     8101 accacccagc gcgataatca cgtctggttt gaaggagttt gccagttctg cacctttacg
     8161 aacgatgctc agggtcgggt ccgcttctac ttcgaagaag acttcagttt caacgcctgc
     8221 tgctttcagt acggaagtga tctgatcagc ataaccattg ttgaacagga agcggtcagt
     8281 cacgatgagc gcacgtttgt ggccatcagt aatcacttca tccagcgcga ttggcaggga
     8341 gccacggcgg aagtagatag atttcggaag tttgtgccac aacatgtttt cagctcgctt
     8401 agcaacggtt ttcttgttga tcaggtgttt cggaccaacg ttttcagaga tggagttacc
     8461 accccaagaa ccacaaccca gagtcaggga aggtgcgagt ttgaagttat acaggtcacc
     8521 gataccaccc tgagacgctg gggtgttaat caggatacga gccgttttca ttttctgacc
     8581 gaagtaagaa acgcgagccg gttggttatc ctggtcagtg tacaggcaag aggtatgacc
     8641 gataccgccc atagcaacca gtttctctgc tttttctacc gcgtcttcga aatctttagc
     8701 gcggtacatt gccagagtcg gggacagttt ttcatgtgcg aacggttcgc tttcatcaac
     8761 aacggtcact tcaccgatca gaatcttggt gttttctggt acagagaagc ctgccagttc
     8821 agcaatttta taggctggct gaccaacgat agccgcgttc agcgcaccgt ttttcaggat
     8881 aacatcctga acagctttca gctctttacc ctgcaacaga tagccgccgt gggttgcaaa
     8941 acgttcacgt acagcgtcat aaacagagtc aacaacaaca acagactgtt cagaagcaca
     9001 gattacgccg ttgtcgaagg ttttggacat cagtacagat gcaactgcac gtttgatatc
     9061 agcagtttca tcgataacaa ctggagtgtt gcccgcgcct acaccgatag ctggtttacc
     9121 ggagctgtat gcggctttaa ccatgcccgg accaccagtc gcgaggatca ggttgatgtc
     9181 tgggtggtgc atcagtgcgt tagacagttc aacagaaggt tgatcgatcc agccgatcag
     9241 atctttcgga gcaccggcag cgatagcagc ctgcagaacg atatcagccg ctttgttggt
     9301 ggcatctttt gcacgcgggt gcggggagaa gataatggcg ttacgggtct tcagactgat
     9361 cagcgatttg aagatagcag ttgaagtcgg gttagtggtc ggaacgatac cgcaaataat
     9421 accgattggt tcagcgatag tgatggtacc aaaagtgtcg tcttcagaca gaacaccaca
     9481 ggttttttca tctttatagg cgttgtagat atattcagaa gcaaagtggt ttttgatcac
     9541 tttatcttcg acgataccca tgccggattc ggcaacggcc attttcgcga gtgggattcg
     9601 agcatctgca gcagccagag cggcggcgcg gaagattttg tctacttgct cttgagtgaa
     9661 actggcatat tcacgctggg ctttttttac acgctctacg agtgcgttaa gttcagcgac
     9721 attagtaaca gccataatgc tctcctgata atgttaaact tttttagtaa atcatctgct
     9781 cgaatacgag agtatagtca gtgcggtgat gatttgctta acctatgaaa atcaaaagct
     9841 tactcgcgct cacactcact gtgatttact aaaagagttt aaacattaga gttattatct
     9901 ctaatgcgtc acttccaggt ggcgtaagca agattactca cttctgggta ctgattacgt
     9961 gatccaaatc aaatttttgc aaagctgaca cctttcagca tcgcttttcg ccattatagc
    10021 taacagttaa taaattgtag tatgatttgg tggctacatt agcatgtttt gcacaactag
    10081 ataacaataa cgaatgatag caattttaag tagttaggag gtgaaaaatg ctgtcaaaag
    10141 gcgtattgtc agcgcgtctt ttcaacctta tttatggcta acattatccg gcttttgctt
    10201 cggagctaac cgtgattcag accttttttg attttcccgt ttacttcaaa tttttcatcg
    10261 ggttatttgc gctggtcaac ccggtaggga ttcttcccgt ctttatcagc atgaccagtt
    10321 atcagacagc ggcagcgcga aacaaaacta accttacagc caacctgtct gtggccatta
    10381 tcttgtggat ctcgcttttt ctcggcgaca cgattctgca actttttggt atatcaattg
    10441 attcgttccg tatcgccggg ggcatcctgg tggtgacgat agcgatgtcg atgatcagcg
    10501 gcaagcttgg cgaggataaa cagaacaagc aagaaaaatc agaaaccgcg gtacgtgaaa
    10561 gcattggtgt ggtgccattg gcgttgccgt tgatggcggg gccaggggcg atcagttcta
    10621 ccatcgtctg gggtacgcgt tatcacagca ttagctatct gtttggtttc tttgtggcta
    10681 ttgcatt
//
LOCUS       DAMZET010000145        10589 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.145, whole genome shotgun sequence.
ACCESSION   DAMZET010000145 DAMZET010000000
VERSION     DAMZET010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.145"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1131)
                     /locus_tag="POB34_003708"
     CDS             complement(<1..1131)
                     /locus_tag="POB34_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cylase"
                     /protein_id="HDI5920578.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVM"
     gene            complement(1312..2787)
                     /locus_tag="POB34_003709"
     CDS             complement(1312..2787)
                     /locus_tag="POB34_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDI5920579.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            complement(2934..4217)
                     /locus_tag="POB34_003710"
     CDS             complement(2934..4217)
                     /locus_tag="POB34_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HDI5920580.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(4330..6264)
                     /gene="yeaG"
                     /locus_tag="POB34_003711"
     CDS             complement(4330..6264)
                     /gene="yeaG"
                     /locus_tag="POB34_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HDI5920581.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            6700..7446
                     /gene="mipA"
                     /locus_tag="POB34_003712"
     CDS             6700..7446
                     /gene="mipA"
                     /locus_tag="POB34_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HDI5920582.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            7536..8390
                     /gene="yeaE"
                     /locus_tag="POB34_003713"
     CDS             7536..8390
                     /gene="yeaE"
                     /locus_tag="POB34_003713"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HDI5920583.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTSLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHHGVMAIPKAATIAHVQQNAAALEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(8441..9325)
                     /locus_tag="POB34_003714"
     CDS             complement(8441..9325)
                     /locus_tag="POB34_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDI5920584.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(9409..10404)
                     /gene="gapA"
                     /locus_tag="POB34_003715"
     CDS             complement(9409..10404)
                     /gene="gapA"
                     /locus_tag="POB34_003715"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDI5920585.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
BASE COUNT         2787 a         2478 c         2548 g         2776 t
ORIGIN      
        1 catcaccgaa gaaccggatt gcaccagttt ctgcaaccgc tgctccagtt caggggttaa
       61 aattttgcga ttatacagtc cagtcatcgc atcactaata ttttctcgcg acacattttg
      121 gtataagcgg aagtgcatcc gcaccatatt cagcaaaatt aacgccgtca aaatccagaa
      181 cagaacgctt ttccacgaag acgtgataaa gtaaagaata tcaatggata acgagacacg
      241 aatgccgccc ggtaagtcat ggacgtaact gacatactgg aacagattat cttcgctctg
      301 gttgatgata atgtcgcgcc ccgaatcggt atcggttagc gtgacattga gaaaacgcca
      361 gaggagaggg cggtcatgag tgtaaaagat attccgcaaa ttgtttttgt taatatccag
      421 cagcacaatc ccttttagct gccctgcaac atacaccggc gtcagcaaac tcatcacgtt
      481 ctgtttagta cgctgatcct ggtaaatgct cgacaatacg gtacgcccgg taaatacctt
      541 ttcaatatct ttgcgatcga taccaatact tcctgactgc aggaaggacc agttattcat
      601 cgcaaagtat tcaacattaa ccggctcata aaaatagaca tagcggtcat tgagatcgag
      661 ataatagtga aatttctctt cggccatccc aagacctgaa ccgtaatcat cctggtggcg
      721 gttgccttcc actgcctgat caaaagcggg taatagtgct gcagcttcca caattgtgtc
      781 gcagtcagtg gatgcggttt gtagtgtgcc gcgtaagccc gggtatttat gggcagtcag
      841 gtttaagcca tgggtattat tggcagactc aaaagcctcg cagatggcgc gccgctgttc
      901 tggcgacact tccgactgct cagcagcgag agcgcgcatc acatgcgcgg caatactctg
      961 attctgatac ttatcataaa gaaaggagga gtctgctttc tgcacgatat agcgcagata
     1021 ggcactcatt gctctgtcgc tggcgacaag ttcaaaaata aggaaggaag atgtcagcac
     1081 aatgacactt gcggcgataa aatgccggag cattctatgg tgcaatttca tgataaatgg
     1141 cactccttcg gttagtgaaa cgagaaaacc agataaagtt cgattgcggc ctggcacagg
     1201 ggcgctaatt atactcatct ctgcgccgct ggggagggga aatttagggg taatagtaat
     1261 aaattgtatg caaaagccac gcaaaaagca tggctctgaa tatttttgca cttaaatatg
     1321 atgaataatc actttgttgc ccccctcgct tttcgcttcg cgtaaggctt tatctgcttc
     1381 cgtcatgaca agcgagatat tacgcgtgtc acccgttgaa aaaaccgtgc caatactgat
     1441 ggtcatttgt tcaggaacat catatctgtt actaaagccg gtcaggagtt cgacattttt
     1501 acgcatacgt tccgcaatga tttttgcctg ggcactattg agttcggtaa atagcaagcc
     1561 aaacacctca ccctccagtc gcgctaaaat atcatcaggg cggatgcttt gctggataat
     1621 atcgacaatt gaaaccagca ctttatcacc cacacgatgt ccccattggg cattgatgcg
     1681 tttgaactga ttaatatcaa ccagcatgac acagaagtcc ttaacaacag gtcgcgacaa
     1741 taatgactct acgctgttga aaaagtagcg ccgattataa atattggtca gcacatcatg
     1801 aactgccagt ttgctggaga gttgcagttc ctgaaaaatg ttataaatca gaaaagacac
     1861 aacaaacagt ttactggaaa cttcaatccc gcgactgata taccagatgg tgtaattata
     1921 ctcatccaga gtcagtagaa gtaagttgca gcatagcgat gccagacata ataatgctat
     1981 taacggccat agttcgctgg ctaatcgtgt acgcatgata ataaagaaca gtaaaaatgc
     2041 ccacagacaa accaatattt ttgtatagtt gattccccag gtcgcagtat ggttgtccgg
     2101 acagtagtcg acgacataca aagaatagtc agcgttataa ctgctcagat tgtgtgccag
     2161 aaggggaaaa actaaaaaag gaatcagcgc cagcaacagg attcccgttt ttttcttatt
     2221 gttgtcaagg atgttgtctt ttccataaca aaagagtgcc agcgaggtta aacaaataaa
     2281 actgagttgg cggaaaatat agaaaataga aacatcattc gactttgtct ggatcagtgt
     2341 actgccgtta attggtttat ggataattac aatggtttca actaaataga taaggccact
     2401 taaaaatgca aggcttaaaa tgagtgttgg taaaatttgt ttgttagtga aatatttcat
     2461 taacatgaaa agaaaaatga cggcgttaag gaaaaacaaa gccacaacaa ccatcaggta
     2521 attcatctgt gggatgagtt ttacatttgt gttttcgtta tataatgtgg tgatgcaaaa
     2581 taaaattaac gtcagcaata aactgaaaat aaaaaagctt tgagctttta atttttgtgt
     2641 acccatagaa accataaaaa ggagagcaat gtgagtgctg gattttattg atggcagaga
     2701 tatatagttt tgaccaggat caattttcgt taacgataaa aaatatttaa agaataaccc
     2761 catggaaata cgtgttgact gaatcatttt tccgaatgat tgttttctgg aactaaatta
     2821 tgtaaaggtt atgctgtcat atttaaagcg attgtaagct aatgtatgta ataaatgaga
     2881 taatttacag taagctaaaa atcagccagg cgataatgac ctggctgata gttttagtct
     2941 ttagctgttg cattttgttt atgaaacagt tcacggaaca ccggataaat atcatcctgg
     3001 tcgcggatgt gctgcatcgc aaagttgtcg aaagtagatt gcagatgctc atattctcgc
     3061 cacaatgtct gatgtgcacg acgggtaatt tcgatatagc tgtaataacg aacaacaggt
     3121 aataattttt tcgccaggat ttcatggcaa agcggagagt catcggccca gttatcgccg
     3181 tccgatgctt gtgcagcgta aatattccac tgtgccgggt tataacgctc tttcactacc
     3241 tcatccatca gtttcagggc gctggaaaca atggtgccgc ctgtttcctg cgagtagaaa
     3301 aactcatgtt catcgacttc tttcgcctgg gtatgatggc ggatgtatac gacttccacg
     3361 ttcttatacg ttctgctgag gaacagatac agcagaatat aaaaacgctt agccatatct
     3421 ttagtggatt gatccattga accggaaacg tccatcaggc aaaacatcac tgcctggctg
     3481 gagggatcgg gccgcttctc gtagttcttg taacgtaaat cgaaggtgtc aataaaaggg
     3541 acgcgttcaa ttttggcacg taattctgca atttctttgc gcagacgttc ctcttccagc
     3601 agctgcgcag gttcactgtt gctgatgatg gccaaattct cttccagtgc atgaagttcc
     3661 cgccgcttgc ctgccgtcat ggctgtgcgt cgcgccagtg agttctgcaa ggagcgcaca
     3721 acgctgatat ttgccggaac gccgttagcg gtataacccg cccgatgcgt tttatattcg
     3781 gtcagctggc gttgttggtt ttgtttcaga ttcggtaagg ccaaatcttc aaagagcaaa
     3841 tcaagatact catctttcga aatctgaaag acaaattcat cctgaccttc accatcctgg
     3901 ctggcctggc cctgaccact gccggaacct ccgccgccac cctggggacg ttcaattcgg
     3961 tcgttctgaa cgaaatggtc attgcccgga tgcacgcggt ggcgcaggcc gccacgcccc
     4021 tgatgaaaca tcggttcgct aatatcttcc gtgggaatgg atacggactc gccgctgtcg
     4081 acgtcagtca ccgaacgctt attaatggcc tcggagatcg actgtttaat ttgcgcttta
     4141 taacggcgta aaaaacgctg gcgattcacc atgcttttgt ttttgccgtt cagacgccgg
     4201 tcaataaacc aggtcatatg ccccccgtac tacatttgcc aactgtatgc tacgcctgtg
     4261 ttgccatttc tctggcccac ggttcgctgt cgttgtaggc ccggtagcat gtgtaccggg
     4321 ccagggttgt taagacgatt tacgtacgcg caaataccat tcgcacagta aacgcacctg
     4381 tttacgggtg tagccttttt ccatcatacg gtcgacaaag tcgtcgtgtt tcttctgctc
     4441 gtcggttgac gttttggcgt taaacgagat aaccggcaac agctcctcgg tattggagaa
     4501 cattttcttc tcgataaccg ttcgcagttt ttcatagctg gtccagttcg gattgcgtcc
     4561 gctgttattc gctctggcgc gcagtacgaa gttaacaatc tcgttgcgga aatcttttgg
     4621 attactgatc cccgccggtt tctcgatttt ctccagctcg gcgttaagag actcgcggtc
     4681 aaacagctgc ccggtatccg gatcgcgata ctcctgatcc tgaatccaga aatccgcgta
     4741 ggtaacataa cggtcgaaaa tgttttgccc atattcggaa taggattcaa ggtaggccgt
     4801 ctggatctct ttgccgataa actcggcata tttcgggatc agataacctt tcaggaactc
     4861 cagatagcgt tctgcctgct cttgtgggaa ctgctcgcgc tcgatctgct gttccaggac
     4921 gtagaacaga tggaccgggt ttgccgccac ttctacatga tcgaagttga acacgcggga
     4981 gaggatctta aacgcaaaac gcgtcgacag accgttcatc ccttcatcga caccggcgta
     5041 gtcacgatat tcctgatacg acttggcttt gggatcggtg tctttcagac tttcgccatc
     5101 ataaacccgc atctttgaat aaatgctgga gttttctggc tctttcaggc gcgaaagaat
     5161 ggaaaaacgt gacagtgttt ccagcgtgcc aggggcgcat ggggcgtgag tcaattcact
     5221 gtgattaagc aatttctcgt agattttgat ctcttcggaa atgcgcaagc aatacggtac
     5281 cttcacgatg taaacacggt cgaggaaggc ttcgttgttt ttgttattac ggaaagtgac
     5341 ccattcggat tcgttcgagt gggcgaggat aatcccgttg aacggcaggg cggagatacc
     5401 ttccgtcccg ttgtagttac cttcctgggt ggcggttaac aagggatgca gcacttttat
     5461 cggtgcttta aacatctcga caaattccat gatcccttgg ttggcgcggc acagcgcacc
     5521 ggaatagccg taggcgtccg ggtcattctg cgcgtagtgt tcgagtttac gaatatcgac
     5581 tttcccaacc agcgcggaga tgtcctggtt gttctcatca ccgggttccg ttttggcgat
     5641 agcaatttgt tgcagaattg acggccagac cttcactacc cggaacttag tgatatcgcc
     5701 accaaattca tgcaggcgtt ttgccgccca cggcgacatg atggtgccga gataacggcg
     5761 aggaatgcca tactcttttt ccagaatctg agcatcttcc tgcggattga aaagacagaa
     5821 cggatgatcg ttgaccgggc tacgctcacc gttggcgctc aatacataaa tcggtacgag
     5881 ctgcattaat gatttcagtc gctcagcaag cgatgattta cccccaccca caggccccag
     5941 cagatacagg atttgtttct tctcttccag cccctgagcc gcgtgtttca gataagagac
     6001 aatctgttca atcgcgtctt ccatgccgta aaactcttca aacgccggat aacgtgcaat
     6061 gacccggtta gaaaagagtc gagaaagtct gggttcctgg gctgtatcga ccatgacagg
     6121 ctcaccgata gccatcaata gccgctcagc cgcgttggca taagcactgc gatcttgccg
     6181 acaagtggta agaaactcct gcagtgtgaa ctcttcgtcc ttggcagctt catatcgctg
     6241 gcgatagtga tcgaatatat tcatggtatg ccgtcctttc gttttttagc acaggttaag
     6301 agccgttcat atgaatagta gaggctcccg gaagaggtaa actgaacgac gtctcttgct
     6361 aaaacacagc aactctcatg ccattttatc gacgatgaga acaacgtgcc cggtgataca
     6421 ccttcaaaat taagcgtaga tggcaaatgg aaaacttgcc tgcacggctt tcttaatttc
     6481 aatggcatat caacaactca tccataaatt ttgcataatt aatgtaaaga ccaggctcgc
     6541 cagtaacgct aaattcattt ggctgtaagc gcggtgtcat ccgcgtcagg aaaattaaac
     6601 agttacttta aaaaatgaaa acgtaaaaag gttgggtttc gatgtattga cgggtaaact
     6661 ttgtcgcccg ctaaacattt gtttaaggaa tgattaattg tgaccaaact caaacttctg
     6721 gcacttggag tgcttatcgc aacgtctgca ggcgtagcgc acgctgaagg taaattttcc
     6781 ctgggcgcag gcgtaggtgt cgttgaacac ccatataaag attacgatac cgatgtttac
     6841 ccagtaccgg taatcaacta tgaaggcgat aacttctggt tccgtggctt aggtggtggt
     6901 tactacctgt ggaatgacgc aacggacaaa ctttcaatta ccgcttactg gtcgccgctt
     6961 tacttcaaag cgaaagacag tggcgatcac caaatgcgtc acctggatga ccgtaagagc
     7021 accatgatgg ctggtctgtc ttatgctcac tttacccagt acggttacct gcgtaccacc
     7081 ctggctggcg ataccctgga taacagcaac ggcattgtct gggatatggc ctggttgtat
     7141 cgttacacca acggcggcct gaccgtgacc ccgggtatcg gtgtgcagtg gaacagcgaa
     7201 aaccagaacg aatactatta tggcgtatcg cgcaaagagt ccgctcgcag cggtctgcgt
     7261 ggctataatc cgaacgacag ctggagccct tacctggagc tgagcgccag ctacaacttc
     7321 ctcggcgact ggagtgttta cggtaccgcg cgctataccc gtctgtctga tgaagttact
     7381 gacagcccga tggtggataa atcctggact ggcctgattt ctaccgggat cacctacaaa
     7441 ttctgataat gcatctttcc agggcatttt gtgcataaaa acagggcgtc acgccctgtt
     7501 ttgcattacg gtggtgcaat acctgggagg aaacaatgca acaaaaaatg attcaattta
     7561 gtggcgatgt ctcactgcca gccatagggc agggaacatg gtatatgggc gaagatgcca
     7621 gtcagcgcaa aacagaagtt gctgcactac gcgcgggcat tgagctcggt ttaaccctca
     7681 ttgataccgc cgaaatgtat gccgatggcg gtgccgaaaa ggtggttggg gaagcattaa
     7741 ccagtctgcg agagaaggtc tttctcgtct ctaaagtcta tccgtggaat gctggcgggc
     7801 aaaaagcgat aaatgcatgc gaagccagtt tacgccgtct caatactgat tatctcgatc
     7861 tctacttatt acactggtct ggcagtttcg cttttgaaga gactgttgca gcgatggaaa
     7921 aattgatcgc ccagggaaaa atccgccgct ggggcgtttc taaccttgat tatgctgata
     7981 tgcaggaact ctggcagctg ccggggggaa atcagtgtgc cactaatcag gtgctttacc
     8041 acctcggttc acgagggatt gagtacgatc tccttccctg gtgccagcaa cagcagatgc
     8101 cggtgatggc ttacagtcca ttagcccagg ccgggcggtt gcgcaatgga ctgttaaaaa
     8161 acgcggtagt caacgaaatt gcacatgctc acaatatcag cgcggcacaa gtattgttgg
     8221 cgtgggtgat cagtcatcat ggtgtgatgg cgattccaaa agcggccacg attgcccatg
     8281 tccaacaaaa tgcagctgcg cttgaggtcg aactttcgtc agcggaatta gctatgctgg
     8341 ataaggcata tccggcacca aaaggaaaaa ctgcgctgga tatggtgtga tgttgatacg
     8401 tgccggatgc atcgcacccg gcacacaaca ttaacgcaaa ttaacgtttg gcaacgcgaa
     8461 tggattgcgc cagatgtgct ggtttctctt tggtgacttt ttgcgtttct gaagcgtaag
     8521 ccgtttctac acaaacaaag gtcttgtagc catcgtccgg catgtcgccc atgctaactg
     8581 aaagcgccgg tcccgggttc cagccgacaa cgttcagatg atgctggtgg cctacggcga
     8641 taatacgatt cagcgcttca tcattaatca cgctgcaatc ttgcggattc agatacacgc
     8701 gatcggtacg gtcagggaag gtctgaatac catcggtcag tacatcttct ttcgcgtcat
     8761 tcactttatc aatgaagcga tcgcccagcc cactgacgct taccttagcg atatcaccca
     8821 cgttaaagta ggtatgcaga gcagaggtgg tttcaaattc gccgtgtgct tcaagatcga
     8881 tttcacaggt tttacccacg cggaaatgcg ctaacagcgt aaagtcgtgc ggccagaatt
     8941 ttttcgtctc ttcgctttgt gtcaattcaa aagtcagcgc tacgccatca gcatcttcac
     9001 gatgtgattt cagcgtccac ggcaggttgc gggcaaaacc gtgcgcaggc aggccttgtt
     9061 gtgccgccgg accaaaccac ggccagcaaa ccggtacgcc accgcgaata gcgacgccat
     9121 ttttgaacgg tgtgttgttg ctcaaccaca gaacttcttc ttcacccgca ggtttccacg
     9181 agagaaggtg tgcgccctgt aatgcaaaag aggcttttac ctggggatga tcgaccacaa
     9241 tgaggtccag ttcatccagt ttacgacggg agaggacagg ggagatttgt tcgatgaccg
     9301 gaagggcaaa aattttctta atcatgacgc agtcctttaa cttcatttta tcaggtaaaa
     9361 aaaagagcga ccgaagtcgc tctttttaga tcacagtgtc atctcaactt atttggagat
     9421 gtgagcgatc aggtccagaa ctttgttgga gtaaccggtt tcgttgtcgt accaggatac
     9481 cagtttcacg aagttgtcgt tcagagcgat accagcttta gcatcgaaca cggaagtgca
     9541 aacttcgccg ttgaaatcgg tagatactac gtcatcttcg gtgtagccca gaacgccttt
     9601 catttcgcct tcagcagcag ctttaacggc agctttgatc tgctcgtaag ttgcagcttt
     9661 ttccagacga acggtcaggt caactacgga tacgttcggg gtcggaacgc ggaacgccat
     9721 accagtcagt ttgccattca gttctggcag tactttacct acagctttag cagcaccggt
     9781 agaggacgga atgatgttct gggaagcgcc gcggccgccg cgccagtctt tgtgagacgg
     9841 gccatcaacg gttttctgag tagcggtagt agcgtgaacg gtggtcatca gaccttcgat
     9901 gatgccgaag ttatcgttga taactttagc cagcggagcc aggcagttgg tggtgcagga
     9961 agcgttggaa acgatgtcct ggccagcata tttgtcgaag ttagcgcctt taacgaacat
    10021 cggagtgttg tctttagacg gaccagtcat aaccactttc ttcgcaccag cggtgatgtg
    10081 tttacgagca gtttcgtcag tcaggaacag accagttgct tcagcgacaa cgtcaacacc
    10141 aacttcgtcc catttcaggt tagccggatc acgttcagcg gtaacacgga tttttttacc
    10201 gttaacgatc agatgaccgt ctttcacttc aacggtaccg tcgaaacggc cgtgagtgga
    10261 gtcatatttc agcatgtatg ccatgtaatc agcgtctaac aggtcgttga ttgcaacgat
    10321 ctcgatgtca gaacgtttct gagcagcacg gaaaacaatg cgaccgatac ggccaaaacc
    10381 gttgatacct actttgatag tcatatattc caccagctat ttgttagtga ataaaaggtt
    10441 gcctgtaaaa ttacaaaaac cttacgcagc gtcaagcgga atcgtgtcaa tcattgcgac
    10501 aaatcaatcc tgtgcctaag cattacgcga ctgactcgcc tcactcttcc tttgggcttg
    10561 agaccacatg gggtcggcgc cccgaattt
//
LOCUS       DAMZET010000146        10568 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.146, whole genome shotgun sequence.
ACCESSION   DAMZET010000146 DAMZET010000000
VERSION     DAMZET010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.146"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..215)
                     /locus_tag="POB34_003716"
     CDS             complement(<1..215)
                     /locus_tag="POB34_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI5920586.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYL"
     gene            438..2306
                     /gene="kup"
                     /locus_tag="POB34_003717"
     CDS             438..2306
                     /gene="kup"
                     /locus_tag="POB34_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HDI5920587.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2473..2892
                     /gene="rbsD"
                     /locus_tag="POB34_003718"
     CDS             2473..2892
                     /gene="rbsD"
                     /locus_tag="POB34_003718"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDI5920588.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPISKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2900..4405
                     /gene="rbsA"
                     /locus_tag="POB34_003719"
     CDS             2900..4405
                     /gene="rbsA"
                     /locus_tag="POB34_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HDI5920589.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLGKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4410..5375
                     /gene="rbsC"
                     /locus_tag="POB34_003720"
     CDS             4410..5375
                     /gene="rbsC"
                     /locus_tag="POB34_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDI5920590.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5400..6290
                     /gene="rbsB"
                     /locus_tag="POB34_003721"
     CDS             5400..6290
                     /gene="rbsB"
                     /locus_tag="POB34_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HDI5920591.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            6416..7345
                     /gene="rbsK"
                     /locus_tag="POB34_003722"
     CDS             6416..7345
                     /gene="rbsK"
                     /locus_tag="POB34_003722"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDI5920592.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7349..8341
                     /gene="rbsR"
                     /locus_tag="POB34_003723"
     CDS             7349..8341
                     /gene="rbsR"
                     /locus_tag="POB34_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HDI5920593.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPALLRLEGYRAAMKRAGLSIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8307..9734)
                     /gene="mdtD"
                     /locus_tag="POB34_003724"
     CDS             complement(8307..9734)
                     /gene="mdtD"
                     /locus_tag="POB34_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HDI5920594.1"
                     /translation="MSDKKNRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9757..10449)
                     /locus_tag="POB34_003725"
     CDS             complement(9757..10449)
                     /locus_tag="POB34_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDI5920595.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2583 a         2542 c         2798 g         2645 t
ORIGIN      
        1 agatattcaa acgcgcgggc attctgaaag gcgaatttta agcgccgggc gatcaaactt
       61 ttggcaatac ctggcgggcc aaggaggaac acactttcac cacttaatgc cgctaataaa
      121 cacaagcgga tggcgtggct acgttcataa agcccctttt ccaacgaact gctcaggcgg
      181 gaaattcttt ccgctaataa atgagggtga gccataatga agtggcgtcc tttcgtcaaa
      241 agttctgcgt aaattgcgag tatagacgtt tcttgctggt ggctaaaata gtctcaaagg
      301 gggggtattt ttctttgagc caggttaatg tggccgcatt taggagtacg attttgccgt
      361 taatcgtgca tactgtgcgc ttttttgtgg gccaagggac taagcacaca tttcatattt
      421 caacgaaaga ctagtctatg agcactgata ataagcaatc attgcccgcg attaccctcg
      481 cggcgattgg agttgtctac ggcgatattg gtaccagccc gttatataca cttcgtgaat
      541 gtttgtccgg ccagtttggt tttggcgttg aacgcgatgc cgtgtttggc tttttatcgc
      601 tgatcttctg gctgctaatc tttgtggttt ccattaaata tctcaccttc gtgatgcggg
      661 cagataacgc cggtgagggg gggatcctga cgttgatgtc gcttgccggg cgtaatacgt
      721 cggcgcgaac cacatcaatg ctggtgatta tggggctaat cggcggcagc tttttctatg
      781 gtgaagtcgt cataacaccc gctatttcgg tgatgtcagc cattgaaggt ctggaaatcg
      841 tcgccccgca gctggatacc tggatagttc ccctctcaat tatcgttctc actttattat
      901 ttatgattca aaaacatggc acggctatgg tcggtaagct gtttgcgccg atcatgctga
      961 cctggttttt gattctggca ggactggggt tacgtagcat tattgctaac ccggaagtgc
     1021 tgcatgcgct gaatccaatg tgggcggtgc atttcttcct tgaatacaaa acggtttctt
     1081 ttattgcatt aggggcagtg gtgttgtcga ttacgggggt cgaggcgctg tatgctgata
     1141 tggggcactt tggtaagttc cctattcgcc tggcgtggtt caccgtcgta ttaccttcct
     1201 tgacccttaa ttacttcggt cagggagcgc tgttgttaaa gaacccggaa gcgattaaga
     1261 acccgttctt cctgttggca ccggactggg cgctgatccc gctgctgatc atcgccgcac
     1321 tggcgacggt aattgcctcg caggcggtta tctctggcgt cttctcattg acgcgtcagg
     1381 cggtacgtct gggatattta tcgccgatgc gcattattca cacctccgaa atggagtcag
     1441 ggcaaatcta tattccgttt gtgaactgga tgctctatgt cgcggtcgtg attgtgattg
     1501 tcagctttga gcactccagc aacctggcgg cggcgtacgg gattgcggtg accggaacca
     1561 tggtgctgac gtctattctc tcgactaccg tggcacgtca gaactggcac tggaataagt
     1621 attttgttgc gctgatcctg attgctttcc tttgtgtcga tattccattg ttcaccgcta
     1681 acctcgataa actgctctcc ggcggctggt tgccattgag cctcggtacg gtgatgttta
     1741 tcgtgatgac cacctggaag agcgagcgtt tccgcttgct gcggcggatg catgaacatg
     1801 gtaactctct ggaagcgatg attgcttcgc tggagaaatc accgcctgtt cgcgtgcccg
     1861 ggaccgcggt gtatatgtcg cgtgcaatca acgtcattcc ctttgcgctg atgcataacc
     1921 ttaaacataa caaggtattg catgagcggg tgattctgtt aactctgcgt accgaagacg
     1981 cgccatatgt ccataatgtc cgtcgggtac agattgaaca actgtcgccc actttctggc
     2041 gcgtggtggc aagttatggt tggcgagaaa cgccaaacgt ggaagaagtt ttccaccgct
     2101 gcggtctgga aggattaagt tgccggatga tggaaacctc cttctttatg tcgcatgagt
     2161 cgttgatcct cggcaaacgc ccgtggtatt tgcgtctgcg cggcaagctg tacttgctgc
     2221 tgcaacgtaa tgcgctgcgt gcaccagatc aatttgaaat cccgccaaac agggttatcg
     2281 aactgggtac tcaggtcgaa atctaacgcc agacgcctcc tttctccata agggggcgtt
     2341 tttgttttca tggttaatca ccatgtaaaa cgtttcgagg ttgatcacat ttccgtaacg
     2401 tcacgatggt tttcccaact cagtgaggat taaactgtgg gtcagcgaaa cgtttcgctg
     2461 atggagaaaa aaatgaaaaa aggcaccgtt cttaattctg atatttcatc ggtgatctcc
     2521 cgtctgggac ataccgatac gctggtggtg tgtgatgctg gtttacccat ctccaaaagt
     2581 acaacgcgta tcgatatggc attaacccag ggtgtacctt cttttatgca ggtgctgggc
     2641 gtcgttacaa atgaaatgca ggtcgaggct gccattatcg cggaagagat caaacaacat
     2701 aatccgcaac tccacgaaac gttgctcact caccttgagc agctgcaaaa acaccaggga
     2761 aataccattg aaattcgtta caccacgcat gaacaattca aacaacaaac cgcagaaagt
     2821 caggcggtaa ttcgcagcgg agaatgttct ccgtatgcga atatcattct ctgtgctggc
     2881 gtgacgttct gaggccgtca tggaagcatt acttcagctt aaaggcatcg ataaagcctt
     2941 cccgggcgta aaagccctct cgggcgcagc gttaaatgtc tatccgggcc gcgtgatggc
     3001 gctggtgggc gaaaacggcg cgggtaaatc caccatgatg aaagtgctta ctggtatcta
     3061 tgctcgcgat gccggcacgc ttttatggct ggggaaagaa acgacattta ccgggccgaa
     3121 atcttcccag gaagccggga ttgggattat ccatcaggaa ctgaacctga tcccgcagtt
     3181 gaccattgcc gaaaacattt tcctcggtcg tgagtttgtt aatcgctttg gcaaaattga
     3241 ctggaaaacc atgtatgccg aagcggataa attgctggct aaacttaacc tgcgctttaa
     3301 aagcgacaag ctggtgggcg atctttccat cggtgaccag caaatggttg aaatcgccaa
     3361 agtgctgagc tttgagtcga aagtcatcat tatggatgaa ccgaccgatg cgctgaccga
     3421 taccgaaacc gaatccctgt tccgcgtcat ccgcgaacta aaatcgcaag gccgcggtat
     3481 tgtctatatc tcccaccgca tgaaagaaat cttcgagatt tgcgatgacg ttaccgtttt
     3541 tcgtgacggg caatttattg ctgagcgcga agtggcatca ctgaccgaag attcgctgat
     3601 tgagatgatg gtgggtcgca agctggaaga tcaatatccg cacctgggca aagcgccggg
     3661 agatatccgc ctgaaagtcg ataatctctg cggacctggc gttaacgatg tctcttttac
     3721 tttacgcaaa ggcgaaattc ttggcgtctc tggtttgatg ggcgcaggtc gtaccgaact
     3781 gatgaaagtg ctctacggcg cactaccgcg caccagcggt tacgtcaccc tggatgggca
     3841 tgaagtcgtt acccgttcac cgcaggatgg cctggcaaac ggcattgtgt atatctccga
     3901 agaccgtaaa cgtgacggtt tagtgttggg catgtcagta aaagagaaca tgtcgctgac
     3961 agcgctgcgc tacttcagcc gcgctggcgg cagtttgaag catgccgatg aacagcaggc
     4021 tgtgagtgat ttcattcgtc tgtttaatgt gaaaacaccg tcgatggaac aggcaattgg
     4081 tctgctttcc ggtggcaatc agcaaaaagt ggcgattgcc cgcggtctga tgacacgccc
     4141 caaagtgttg atccttgatg aacctacccg tggcgtagat gtcggcgcga aaaaagagat
     4201 ctatcaactg attaaccagt tcaaagccga tggcttgagc atcattctgg tgtcatcgga
     4261 gatgccagaa gtattaggca tgagcgatcg catcatcgtc atgcatgaag ggcatctcag
     4321 cggggaattt actcgtgagc aggccaccca ggaagtgtta atggctgccg ctgtgggcaa
     4381 gcttaatcgc gtgaatcagg agtaaaaaaa tgacaaccca gactgtctct ggtcgccgtt
     4441 atttcacgaa agcgtggctg atggagcaga aatcgcttat cgctctgctg gtgctgatcg
     4501 cgattgtctc gacgttaagc ccgaactttt tcaccatcaa taacttattc aatattcttc
     4561 agcaaacctc ggtgaacgcc attatggcgg tcgggatgac gctggtgatc ctgacgtcgg
     4621 gtatcgactt gtcggtaggt tctctgttgg cgctgaccgg cgcagttgct gcatctatcg
     4681 tcggcattga agtcaatgcg ctggtggctg tcgctgccgc tctcgcgtta ggtgccgcaa
     4741 ttggtgcggt aaccggggtg attgtagcga aaggtcgcgt tcaggcgttt atcgctacgc
     4801 tggttatgat gcttttactg cgcggcgtga ccatggttta taccaacggt agcccagtga
     4861 ataccggctt tactgagaac gccgatctgt ttggctggtt tggtattggt cgtccgctgg
     4921 gcgtaccgac gccagtctgg atcatgggga ttgtcttcct cgcggcctgg tacatgctgc
     4981 atcacacgcg tctggggcgt tacatctacg cgctgggcgg caacgaagca gcaacgcgtc
     5041 tttctggtat caacgtcaat aaaatcaaaa tcatcgtcta ttctctttgt ggtctgctgg
     5101 catcgctggc cgggatcatt gaagtggcgc gtctctcctc cgcacaaccc acggcgggga
     5161 ctggctatga gctggatgct attgctgcgg tggttctggg cggtacgagt ctggcgggcg
     5221 gaaaaggtcg cattgttggg acgttgatcg gcgcattaat tcttggcttc cttaataatg
     5281 gattgaattt gttaggtgtt tcctcctatt accagatgat cgtcaaagcg gtggtgattt
     5341 tgctggcggt gctggtagac aacaaaaagc agtaataacg actacaggac atcttgaata
     5401 tgaacatgaa aaaactggct accctggttt ccgctgttgc gctaagcgcc accgtcagtg
     5461 cgaatgcgat ggcaaaagac accatcgcac tggtggtctc cacgcttaac aacccgttct
     5521 ttgtatcgct gaaagatggc gcgcagaaag aggcggataa gcttggctat aacctggtgg
     5581 tgctggactc ccagaacaac ccggcgaaag agctggcgaa cgtgcaggac ttaaccgttc
     5641 gcggcaccaa aattctgctg attaacccga ccgactccga cgcagtaggt aatgctgtga
     5701 agatggctaa ccaggcgaac atcccggtta tcactcttga ccgccaggca acgaaaggtg
     5761 aagtggtgag ccacattgct tctgataacg tactgggtgg caaaatcgcc ggtgattaca
     5821 tcgcgaagaa agcgggtgaa ggtgccaaag ttatcgagct gcaaggcatt gctggtacat
     5881 ccgcagcccg tgaacgtggc gaaggcttcc agcaggccgt tgctgctcac aagtttaatg
     5941 ttcttgccag ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     6001 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     6061 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     6121 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     6181 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     6241 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6301 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6361 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6421 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6481 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6541 caaaggtgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6601 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6661 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6721 taatggcgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6781 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6841 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6901 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6961 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     7021 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     7081 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     7141 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     7201 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     7261 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7321 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7381 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7441 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7501 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7561 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7621 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7681 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7741 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7801 tggcgacagc gatcttattc aggataactc gttgctgggc ggagacttag caacgcaata
     7861 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7921 ggcgctcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcagcattcc
     7981 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     8041 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     8101 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     8161 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     8221 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     8281 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8341 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8401 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8461 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8521 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8581 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8641 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8701 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8761 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8821 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8881 ataaggaacg gtacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatc
     8941 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     9001 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     9061 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     9121 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     9181 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     9241 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9301 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9361 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9421 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9481 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9541 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9601 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9661 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgattc
     9721 tttttatcgc tcattcaatg tcctgttatc gggttgtcac ttatcaggtg agcgtagcag
     9781 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9841 ctggtgcaga tccagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9901 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9961 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
    10021 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
    10081 tgcagccagt aggcaagctt gcggctccag acagatacgc ataaccagaa agtgatcgat
    10141 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
    10201 ccagtttgat tgtggcatga cccgggtacc aattcgcggt cgcggtaaaa ccatcccttt
    10261 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10321 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatgcgttg
    10381 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10441 taaaggcatt actaatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10501 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gcacattaac ggggcttttg
    10561 ctgaaaaa
//
LOCUS       DAMZET010000147        10505 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.147, whole genome shotgun sequence.
ACCESSION   DAMZET010000147 DAMZET010000000
VERSION     DAMZET010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.147"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..569)
                     /locus_tag="POB34_003726"
     CDS             complement(<1..569)
                     /locus_tag="POB34_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HDI5920596.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVV"
     gene            complement(674..1474)
                     /gene="tcyJ"
                     /locus_tag="POB34_003727"
     CDS             complement(674..1474)
                     /gene="tcyJ"
                     /locus_tag="POB34_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI5920597.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTLKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(1562..2113)
                     /gene="fliZ"
                     /locus_tag="POB34_003728"
     CDS             complement(1562..2113)
                     /gene="fliZ"
                     /locus_tag="POB34_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HDI5920598.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDALLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(2159..2878)
                     /gene="fliA"
                     /locus_tag="POB34_003729"
     CDS             complement(2159..2878)
                     /gene="fliA"
                     /locus_tag="POB34_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HDI5920599.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGKLEQELGRNATETEVAERLGIDIANYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(3199..5211)
                     /locus_tag="POB34_003730"
     CDS             complement(3199..5211)
                     /locus_tag="POB34_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HDI5920600.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLSKDGSMKIQVGANDGETITIDL
                     KKIDSDTLNLAGFNVNGKGSVANTAATSDDLKLAGFTKGTTDTNGVTAYTNTISNDKA
                     KASDLLANITDGSVITGGGANAFGVAAKNGYTYDAASKSYSFAADGADSAKTLSIINP
                     NTGDSSQATVTIGGKEQKVNISQDGKITAADDNATLYLDKQGNLTKTNAGNDTAATWD
                     GLISNSDSTGAVPVGVATTITITSGTASGMSVQSAGAGIQTSTNSQILAGGAFAAKVS
                     IEGGAATDILVASNGNITAADGSALYLDATTGGFTTTAGGNTAASLDNLIANSKDATL
                     TVTSGTGQNTVYSTTGSGAQFTSLAKVDTVNVTNAHVSAEGMANLTKSNFTIDMGGTG
                     TVTYTVSNGDVKAAANADVYVEDGALSANATKDVTYFEQKNGAITNSTGGTIYETADG
                     KLTTEATTASSSTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEA
                     QSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            5477..6883
                     /gene="fliD"
                     /locus_tag="POB34_003731"
     CDS             5477..6883
                     /gene="fliD"
                     /locus_tag="POB34_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDI5920601.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRADTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDANGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            6908..7318
                     /gene="fliS"
                     /locus_tag="POB34_003732"
     CDS             6908..7318
                     /gene="fliS"
                     /locus_tag="POB34_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDI5920602.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            7318..7683
                     /gene="fliT"
                     /locus_tag="POB34_003733"
     CDS             7318..7683
                     /gene="fliT"
                     /locus_tag="POB34_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HDI5920603.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWEELIASEMAYVNAV
                     QEIAHLTEEIDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            7761..9248
                     /gene="amyA"
                     /locus_tag="POB34_003734"
     CDS             7761..9248
                     /gene="amyA"
                     /locus_tag="POB34_003734"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDI5920604.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(9282..9695)
                     /locus_tag="POB34_003735"
     CDS             complement(9282..9695)
                     /locus_tag="POB34_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDI5920605.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            9882..>10505
                     /locus_tag="POB34_003736"
     CDS             9882..>10505
                     /locus_tag="POB34_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HDI5920606.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMVVFFGML"
BASE COUNT         2580 a         2688 c         2563 g         2674 t
ORIGIN      
        1 accaccgacg aaatattaac cgccccttca cactgttgcg cgatttccag cgcactctcc
       61 acataaccca gcgcgcccag agcattagaa ccgccaaccg gaatgacata cgggcgaaag
      121 ccttgtgctt cgactcgcgt cgccagctct tccagttggg cattgggatc ggtcagtgcg
      181 tcgcacattt caatctgggt attgaacaga tccagcaaca aacgattgcc gttggttaaa
      241 tagttttctg cggttgtgcc aataggattt tccagcagcg ccacgcagtg cagaccgagt
      301 ttcgccgcaa ctgcggcagt ctggcgcaca tggttagact ggatcgcccc ggcagtaatc
      361 agcgtatcgg caccttcgcg cagagcatct gctgcgagaa attccagctt acgtaattta
      421 ttgccgccca ttgccatggg ggtgacgtca tcccgtttga tgaaaatttc ccgtccaaga
      481 taatcagaaa agcgcggcag atattcgagc ggcgttggcg cgccgataaa ctccaaccgt
      541 ggaaaacggg ttaaattatg cagtggcata acagcctccg atgtgtgttg ttgtgatttt
      601 cttattatgc acgctgaaaa cgcgtaaata aaaaaggcgc tagtgaaagc gccctttttt
      661 gtcattatgc tgattatttg gtcacatcag caccaaacca tttttcggaa agagcttgca
      721 gagtgccgtc tttttgcatt tccgcaattg catcattcac cgctttcagc aggtcctcat
      781 ttcctttacg cagcgccacg ccagactcct gacgggagaa tgcttcaccg gttactgcca
      841 gcgtatcgtt ggttttcttc accagatcca gcgccgccag acgatcaact aggatcgcat
      901 cgatacgccc tacgcgcaga tcctgatatt tggtcgggtc atcatcatag gtacgcacat
      961 cgacaccctg aacattctgc cgcagccact cttcatagtt ggtgcccaga ccgacaccca
     1021 cttttttgcc tttcagatca gcagccgttt taagggtgcc ttcgttacct tttttcacca
     1081 gcgcctgaat accggaaatg gtgtaagggg tagagaaatc gtattttttc ttgcgctcgt
     1141 cagaaatggt gacctgatta atcaccacat caatgcgttt agagtccagc gacgccagca
     1201 taccgtccca tttggtcggt tttagtgacg cctcaacgcc aagatgtttt gccagctgtt
     1261 gggcaaattc cacttcaaaa ccggttaatt tgccgtcatc cccctgaaaa ctgaacggcg
     1321 gataagttcc ttccagccct accagtagcg taccgcgctc tttgacttta ttaagcagac
     1381 cttcatctgc aaaactttta acgctcatgc ccgcaaccag cgccacggcc atcacaccca
     1441 tcaatgcctg acgtcccaga tgtgctaatt tcatattcac cccgaatgtt gttatgtctg
     1501 tttgcagtgt agagccattg tttgtaaaca caaaaacaac tccgctacat cttattctta
     1561 tttaatatat atcagaagca ggcaggctgg aggatttctg cacaagtccg gtacacgttt
     1621 gacgctgata gtgttgatac ttccgtaatg cgatgcggta attgttggtg ctggtggtgg
     1681 gcagccacgg ggcgaggatt tcatcaagga aaccgtcctg cagctgatcg agggaaatat
     1741 tttgctcgtg tagatgattc cccaaacggc gcaaacgaac gacatattca cgcaccgtac
     1801 ctgggctcat ttcagtttgc tcaaaaagaa attgcttaaa gccgataata tcgaaaaaat
     1861 cactctgcgt tttgcaatgt aaatcaccgc aaaatcggca caatgccgcc catgattttt
     1921 gttccgtttg ccagccattt tcatcaagca gcgcgtccag gcgggaaatc tcgattttat
     1981 tcactatttt gccgtcgcga acgagggtaa tgcgatcgag taatttacgg caatgcgcgc
     2041 aatgagtctg gctgtgttta aagtctttaa gatagcggct taatggccgt cttttcaggt
     2101 gctgcaccat cattaagaac tcctggtagt caaaattaaa gtgcggcatt tactgacgtt
     2161 ataacttacc cagtttagtg cgtaaccgtt taatggcctg gctgtgtaac tgactgaccc
     2221 gcgattcccc gacctccagc accgcgccaa tctctttgag attcagctct tcctggtaat
     2281 agagggttaa taccagtttt tcgcgctccg gcaacgtttc gatagcttcc atcacccgct
     2341 ggcgcagatt actgtccagc agttgttgta gcgggttttc tcgctgatga tcatcagtaa
     2401 ccagttcgat gctatcgccg tgctcttcgc gccactcatc gtaggagaag agctggctgt
     2461 tattggtgtc gagcagcatt tgacgataat tggcaatatc gatccctaaa cgttccgcta
     2521 cctcagtttc cgtggcgttg cggccaagtt cctgctccag tttccctatt gcctgtgcca
     2581 cttcacgcgc gttgcgtcgc acgctgcgcg gcacccagtc acggctgcga agttcatcca
     2641 gcatagcgcc acggatacgc tgcactgcgt aagttgtaaa tgccgttcct tgtagggcgt
     2701 catagcgttc gacggcatta agtaacccaa tgccgcccgc ctgtagcaga tcgtcaagtt
     2761 ccacgctcgc gggcagtcga acctgcaggc gcaatgcttc gtgacgcacc agcgggacat
     2821 aacgctgcca cagcgagtgt ttatccatta caccttcagc ggtatagagt gaattcacga
     2881 taaacagccc tgcgttatat gagttatcgg catgattatc cgtttctgca gggtttttaa
     2941 tcggacgatt agtgggtgaa atgaggggtt atttgggggt taccggtaaa ttgcggacag
     3001 aaaaaaaccc cgccggtggc ggggaagcac gttgctgaca aattaccatt catgttgccg
     3061 gatgcggcgt aaacgcctta tccggcctac aaaaatgtgc aaattcaata aattgcagtt
     3121 caccttgtag gcctgataag cgcagcgcat caggcaattt ggcgttgccg tcagtctcag
     3181 ttaatcaggt tacgacgatt aaccctgcag cagagacaga acctgctgcg gtacctggtt
     3241 agcttttgcc agcacggagt taccggcctg ctggatgatc tgcgctttcg acatgttgga
     3301 cacttcggtc gcatagtcgg cgtcctgaat acgggactgc gcttcggaca ggttggtagt
     3361 ggtgttgttc aggttggtga ccgcggaatc cagacggttt tgcaccgcac cgagggagga
     3421 gcggaatttg tcgatggagc tgatggcttc gtccagagct ttcaggggat cggcggtgga
     3481 actggatgca gtagtagctt ctgttgttaa cttaccatca gctgtttcat agatggtacc
     3541 accggtgctg ttggtaatag ccccattttt ttgttcaaag taggttacat cttttgtagc
     3601 attggctgaa agtgcaccat cttcgacata aacatcagca tttgcagcag ctttcacatc
     3661 cccattggaa actgtgtaag ttactgtacc tgtaccgccc atatcaatgg taaaattgct
     3721 ttttgtcaga tttgccatac cttcggcact gacatgtgcg ttggtgacat tgactgtgtc
     3781 tacttttgct aaactggtga actgagcgcc acttcctgtt gtgctataaa cagtgttctg
     3841 gccggtacct gaagttacgg ttaaggtagc atccttactg ttagcaatta aattatctaa
     3901 cgaagcagct gtatttcctc cagccgttgt agtgaatcca ccagtagtcg catcaagata
     3961 aagtgcacta ccatcagccg ctgttatgtt tccattactt gctaccaaaa tgtctgtagc
     4021 agcgcctccc tcaatactta ccttagccgc aaatgcacca cctgcaagaa tctgagaatt
     4081 tgttgaggtc tgaattcctg ctcctgcgga ctgaacagac attccggaag ctgtaccaga
     4141 agtaattgta attgtagttg caaccccaac tggaaccgca ccggtagaat cgctgttgga
     4201 aattaaacca tcccaagtcg ctgcggtatc gttacctgca ttcgtttttg tcaagtttcc
     4261 ctgtttatct aaatacagcg tcgcattatc atctgccgca gtaatttttc catcctggga
     4321 aatattaact ttctgctctt taccaccaat agtcactgtc gcctgcgacg aatcaccggt
     4381 gtttggatta atgatgctta acgtcttcgc tgaatcggca ccatctgcag caaaactata
     4441 agatttactt gctgcatcat aggtgtaacc attctttgca gccacgccaa aagcgtttgc
     4501 ccctccccca gtgatcactg atccatcggt gatattagct aacagatcgg aagctttggc
     4561 tttgtcatta ctaattgtgt ttgtatacgc ggtcacgcca ttggtatctg tggtgccctt
     4621 agtgaaacca gccagtttta aatcgtcgct tgtcgcagct gtattcgcta cagaaccttt
     4681 accgttaacg ttaaaaccag ccagattcag cgtatcagag tcaattttct tcagatcaat
     4741 agtaatcgtt tcgccatcgt tcgcgccgac ctgaattttc atcgagccat ctttggacag
     4801 cacgttcacg ccgttgaact gggtctggcc ggatacgcgg tcaatttcgt ccagacggga
     4861 tttgatttcg tcctgaatgg agtccagatc cgaatcggag ttagtcccgg tagaagcctg
     4921 aaccgtcagt tcacgaatac gctgtaagtt gttgttaatt tcggacagcg cgccttcagt
     4981 ggtctgtgca acggaaatac cgtcgttggc gttacgtgca gcctgagtca ggcctttaat
     5041 gttagaagta aaacggttag caatcgcctg acccgcggcg tcatccttcg cgctgttaat
     5101 acgcaagcca gaagacagac gctcgataga actcgacagc gcagactggt tcttgttgat
     5161 attattttga gtgatcagcg agaggctgtt ggtattaatg acttgtgcca tgattcgtta
     5221 tcctatattg caagtcgttg attacgtatt gggtttccac ccgtcggctc aatcgccgtc
     5281 aaccctgtta tcgtctgtcg taaaacaacc tttagaattt ttttcacaaa cagccatttt
     5341 ttgttagtca ccgaaatact cttttctctg ccccttattc ccgctattaa aaaaaacaat
     5401 taaacgtaaa ctttgcgcaa ttaaggccga taaccccggt attcgtttta cgtgtcgaaa
     5461 gataaaagga aatcgcatgg caagtatttc atcgctggga gtcgggtcag gtctggattt
     5521 aagttcaatc cttgatagcc tcaccgccgc gcaaaaagcg acgctaaccc ccatttcaaa
     5581 tcagcaatcg tcgtttaccg ctaaacttag cgcctacggt acgctgaaaa gcgcgctgac
     5641 gactttccag accgccaata ctgcattgtc taaagccgat cttttttccg ccaccagcac
     5701 taccagcagt accaccgcat tcagtgccac cactgcgggt aacgccatcg ccgggaaata
     5761 caccatcagt gtcacccatc tggcgcaggc gcaaaccctg accacgcgca ccaccagagc
     5821 cgatacgaaa acggcgatcg ccaccagcga cagtaaactc accattcaac aaggcggcga
     5881 caaagatccg attaccattg atatcagcgc ggctaactca tcgttaagcg ggatccgtga
     5941 tgccatcaac aacgcaaaag caggcgtaag cgcaagcatc attaacgtgg gtaacggtga
     6001 atatcgtctg tcagtcacat caaatgacac cggccttgat aatgcgatga cactctcggt
     6061 cagcggtgat gatgcgctac aaagttttat gggctatgac gccagtgcca gcagcaacgg
     6121 tatggaggtc tcggttgccg cccagaatgc gcagctgacg gtcaacaacg tcgccatcga
     6181 gaacagcagc aacaccatca gcgacgcgct ggaaaacatc accctgaacc tgaacgatgt
     6241 caccacgggc aaccagacgc taaccatcac tcaggacacc tccaaagcgc aaacggcgat
     6301 taaagactgg gtgaatgcct ataactcgct aatagatacc ttcagcagcc tgaccaaata
     6361 caccgccgta gatgcgggag ctgatagcca gagttctagc aatggcgcac tgctcggcga
     6421 ctccacgctg cggacgattc agacgcagtt gaaatcgatg ctgagtaata ccgtcagttc
     6481 ttccaactat aaaacgctgg cacagattgg tatcacgacc gatcccagcg atggaaaact
     6541 ggaactggat gccgacaaac tcaccgctgc actgaaaaaa gatgccaacg gcgtaggtgc
     6601 attgattgtt ggcgatggta aaaaaaccgg catcacaacc accatcggca gcaacctgac
     6661 cagttggctt tcgacaacgg gcattattaa agccgctacc gatggcgtta gtaaaaccct
     6721 gaataagtta actaaagact acaacgccgc cagcgatcgc attgatgcgc aggtcgcgcg
     6781 ctacaaagaa caatttaccc aactggacgt tttaatgacc tcgttaaaca gcaccagcag
     6841 ctacttaacg cagcagttcg aaaacaacag taattccaag taatcattat tcatcgggag
     6901 acaggtcatg tacgcggcaa aaggcaccca ggcctatgca caaattggcg tcgaaagcgc
     6961 cgtaatgagc gccagccagc agcagctggt caccatgcta tttgatggag tgctgagcgc
     7021 actggttaga gcgcgcctgt ttatgcagga caacaatcag caaggcaaag gcgtctcttt
     7081 gtcaaaagcg atcaacatca ttgagaacgg actgcgggtg agtcttgatg aagagagcaa
     7141 agacgaatta acccaaaact tgattgctct ttatagctat atggtcaggc gcttgctgca
     7201 agccaattta cgcaacgatg tctccgcagt tgaagaagtg gaagcattaa tgcgcaatat
     7261 tgccgatgcc tggaaagagt cgttactctc cccttctttg attcaggacc cagtctgatg
     7321 aaccacgcac cgcatttgta tttcgcctgg caacaactcg tcgaaaaaag ccagctcatg
     7381 ttacgcctgg caacggaaga acaatgggaa gaactcatcg ccagcgaaat ggcgtatgtg
     7441 aatgcggtgc aggagattgc acatctgact gaagagattg acccgtccac cacgatgcag
     7501 gagcagctcc gcccgatgct gcgcctgatt ctcgacaacg aaagcaaggt aaagcagtta
     7561 ttacagattc ggatggatga actggcgaaa ctggtcggtc agtcatcggt gcaaaaatcg
     7621 gtgttaagtg cctatggcga tcagggcggc tttgtgctgg ctccgcagga taacctcttt
     7681 tgaatctgaa cgagtcgatg gctcgcgaat aatccgatta cggctacgct tctaatgttc
     7741 cccttgaatg gagtcgaaga atgcgtaatc ccacgctgtt acaatgtttt cactggtatt
     7801 acccggaagg cggtaagctc tggcctgaac tggccgagcg cgccgacggt tttaatgata
     7861 ttggtatcaa tatggtctgg ttgccgcccg cctataaagg cgcatcgggc gggtattcgg
     7921 tcggctacga ctcctatgat ttatttgatt taggcgagtt tgatcagaaa ggcagcatcc
     7981 ctactaaata tggcgataaa gcacaactgc tggccgccat tgacgccctg aaacgtaatg
     8041 acattgcggt gctgttggac gtggtagtca accacaaaat gggcgcggat gaaaaagaag
     8101 ctattcgcgt gcagcgtgta aatgctgacg accgtacgca aattgatgaa gaaatcattg
     8161 agtgcgaagg ctggacgcgt tacaccttcc ccgcccgtgc cgggcaatac tcgcagttta
     8221 tctgggattt caaatgtttt agcggtatcg accatatcga aaaccctgat gaagatggca
     8281 tatttaaaat tgttaacgac tacaccggcg aaggctggaa cgatcaggtt gatgatgaat
     8341 taggtaattt cgattatctg atgggcgaga atatcgattt tcgcaatcat gccgtgacgg
     8401 aagagattaa atactgggcg cgctgggtga tggaacaaac gcaatgcgac ggttttcgtc
     8461 ttgatgcggt caaacatatt ccggcgtggt tttataaaga gtggatcgaa cacgtacagg
     8521 aagttgcgcc aaagccgctg tttattgtgg cggagtactg gtcgcatgaa gtcgataaac
     8581 tgcaaacgta tattgatcag gtggaaggca aaaccatgct gttcgatgcg ccgctgcaga
     8641 tgaaattcca tgaagcatcg cgcatggggc gcgactacga catgacgcag attttcacgg
     8701 gtacattagt ggaagccgat cctttccacg ccgtgacgct cgttgccaac cacgacaccc
     8761 aaccgttgca agctctcgaa gcgccggtcg aaccgtggtt taaaccgctg gcgtatgcct
     8821 taattttgtt gcgggaaaat ggcgttcctt cggtgttcta tccggacctc tacggtgcgc
     8881 attacgaaga tgtcggtggt gacgggcaaa cctatccgat agatatgcca ataatcgaac
     8941 agcttgatga gttaattctc gcccgtcagc gtttcgccca cggtgtacag acgttatttt
     9001 tcgaccatcc gaactgcatt gcctttagcc gcagtggcac cgacgaatat cccggctgcg
     9061 tggtggtcat gtcgaatggg gatgatggcg aaaaaaccat tcatctggga gagaattacg
     9121 gcaataaaac ctggcgtgat tttctgggga accggcaaga gagcgtagtg accgacgaaa
     9181 acggcgaagc aaccttcttt tgcaacggcg gcagcgtcag cgtgtgggtt atcgaagagg
     9241 tgatttaaat tcatccccgg cggcaagccg gggagatttc attacggcag tggcgtcggt
     9301 aacggcgctt tatccagcgc ggcggcacac tctgctgtcg ggcgatccac acgctccatc
     9361 gtcataccat cgtactcaat ggtgttacca tcacgctcaa cctcgtacag ctcacgtttt
     9421 acggtaacgt tagtgagatc atcagacatc aatgtcaact tacccggtac ggcaatcacc
     9481 cgctgccact gacggcaatc gagggtatcg ccctctttag tgacaatcag gctgcctatt
     9541 gcttccggac tgaccagcgc acgttgcggc cctttggtct gccagtagcc cgccagccag
     9601 tccggcgcgg gggttttcac gacattgtta tagttttcga cttcggcaca gcccgccagc
     9661 aacatgagcg cgcctgcaat tgcgagtttt ttcatcattc gtcctgcatg agaagaaaag
     9721 aggattgtgg cattaaagcc ctgatgtcgc cagcctttcc aggttcagag agaaatttga
     9781 tctaactatt atttttgtga attattaccc cgaaaataaa tttgcatggt gtatgattcg
     9841 cgactcttgt ttcaaacctt ctgagttcag aggctacatt catgtcatgg cagcaattca
     9901 aacacgcctg gttgattaaa ttctgggcgc ccatccccgc ggtcatcgcg gcgggtattc
     9961 tctccactta ctattttggc attactggca ccttttgggc tgtcacgggt gaatttaccc
    10021 gttggggcgg ccagctcctg caactgttcg gcgtccatgc tgaagagtgg ggttacttta
    10081 aaattatcca tctggaagga tcgccattaa cccgcatcga cgggatgatg atcctcggta
    10141 tgtttggcgg ctgctttgcc gcagcgctgt gggccaacaa tgtcaaactg cgaatgccgc
    10201 gcagccgtat ccgcattatg caggccatca ttggcggcat tatcgccggt tttggcgcgc
    10261 gtctggcaat gggctgtaac ctggcggcgt tctttaccgg tattcctcag ttctcgctgc
    10321 atgcctggtt ctttgccatc gccactgcca ttggttcatg gtttggcgcg cgctttaccc
    10381 tgctgcccat cttccgtatt cccgtgaaaa tgcagaaagt ttctgccgcc tcaccgctga
    10441 cgcaaaaacc ggatcaggcg cggcgtcgtt ttcgtctcgg gatggtggtc tttttcggca
    10501 tgctg
//
LOCUS       DAMZET010000148        10432 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.148, whole genome shotgun sequence.
ACCESSION   DAMZET010000148 DAMZET010000000
VERSION     DAMZET010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.148"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..707
                     /gene="dadA"
                     /locus_tag="POB34_003737"
     CDS             <1..707
                     /gene="dadA"
                     /locus_tag="POB34_003737"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HDI5920607.1"
                     /translation="DCQLFTQNLARMAEQAGVKFRFNTPVDQLLCDGEQIYGVKCGDE
                     VIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYSLTIPIAQEDGAPVSTILDETYKIA
                     ITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMVVRDLYPRGGHVEQATFWTGLRPMT
                     PDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQLLSDLLSGRTPAIPYEDLSVARYS
                     RGFTPSRPGHLHGAHS"
     gene            717..1787
                     /gene="dadX"
                     /locus_tag="POB34_003738"
     CDS             717..1787
                     /gene="dadX"
                     /locus_tag="POB34_003738"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HDI5920608.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(2173..3909)
                     /locus_tag="POB34_003739"
     CDS             complement(2173..3909)
                     /locus_tag="POB34_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDI5920609.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(4004..4918)
                     /gene="ldcA"
                     /locus_tag="POB34_003740"
     CDS             complement(4004..4918)
                     /gene="ldcA"
                     /locus_tag="POB34_003740"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HDI5920610.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIHRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            5018..5629
                     /gene="emtA"
                     /locus_tag="POB34_003741"
     CDS             5018..5629
                     /gene="emtA"
                     /locus_tag="POB34_003741"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HDI5920611.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(5631..6365)
                     /locus_tag="POB34_003742"
     CDS             complement(5631..6365)
                     /locus_tag="POB34_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HDI5920612.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            6566..6820
                     /locus_tag="POB34_003743"
     CDS             6566..6820
                     /locus_tag="POB34_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDI5920613.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGIFRLLRRE"
     gene            6998..7438
                     /locus_tag="POB34_003744"
     CDS             6998..7438
                     /locus_tag="POB34_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920614.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(7517..9214)
                     /gene="treA"
                     /locus_tag="POB34_003745"
     CDS             complement(7517..9214)
                     /gene="treA"
                     /locus_tag="POB34_003745"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDI5920615.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPHQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYMWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(9534..>10432)
                     /locus_tag="POB34_003746"
     CDS             complement(9534..>10432)
                     /locus_tag="POB34_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDI5920616.1"
                     /translation="RLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKRQRFNRQG"
BASE COUNT         2554 a         2585 c         2731 g         2562 t
ORIGIN      
        1 gagactgcca gctgtttacc cagaatctgg cgcggatggc agagcaggcg ggggttaaat
       61 tccgctttaa tacgcccgtt gaccaactgc tttgcgacgg cgagcaaatc tacggcgtga
      121 agtgtggcga tgaagtgatt aaggccgatg cgtatgtgat ggcgtttggt tcttactcga
      181 cggcgatgct caaaggcatt gttgatattc cggtgtatcc gctgaaaggc tactcgctga
      241 ctattccaat tgcgcaggaa gatggtgcgc cagtatccac tattcttgat gaaacctaca
      301 aaatcgccat tacccgtttc gataaccgca ttcgtgttgg cggaatggcg gaaattgttg
      361 gttttaacac cgagctgttg caaccgcgtc gtgaaacgct ggaaatggtg gttcgcgatc
      421 tctatccacg cggcggtcat gtcgagcagg cgactttctg gacgggtttg cgcccgatga
      481 cgccagacgg tacgccggtt gtcgggcgta cacgctttaa aaatctgtgg ctgaataccg
      541 gtcacggcac gctcggctgg acgatggctt gcggttccgg tcagttgtta agcgatctgc
      601 tctctggtcg cacgccagcg atcccatatg aggatctaag cgtagcgcgc tacagccgtg
      661 gatttacgcc atcacgtccg ggccatttac atggcgcaca cagctaagga aacgagatga
      721 cccgtccgat acaggccagc ctcgatctgc aggcattaaa acagaatctg tccattgtcc
      781 gccaggccgc gccgcacgcg cgcgtctggt cggtggtaaa agcgaacgct tacgggcatg
      841 gtattgagcg tatctggagc gcgctcgggg ccaccgatgg ctttgcatta cttaacctgg
      901 aagaggcaat aacgttacgt gagcgcggct ggaaggggcc gatcctgatg ctggaaggat
      961 ttttccatgc tcaggatctg gagatttatg accagcaccg cctgaccacc tgcgtacaca
     1021 gcaactggca gctcaaagca ctgcaaaatg cgcggctaaa agcaccgttg gatatttatc
     1081 ttaaagtgaa cagtgggatg aatcggttgg gcttccagcc cgatcgcgtg cttaccgtct
     1141 ggcagcagtt gcgggcaatg gcgaatgttg gcgagatgac cctgatgtcg cattttgccg
     1201 aagcggaaca tcctgatgga atttccagcg cgatggcgcg tattgagcag gcggcggaag
     1261 ggctggagtg tcggcgttcg ttgtccaatt cggcggcgac tctgtggcac ccggaagcgc
     1321 attttgactg ggttcggcct ggcattattt tgtatggcgc ttcgccgtcc ggtcagtggc
     1381 gtgatatcgc caataccgga ttacgtccgg tgatgactct aagcagtgag attattggtg
     1441 tccagacgct aaaagcgggt gagcgtgtgg gctacggcgg tcgctatact gcgcgcgatg
     1501 aacagcgaat cggcattgtc gccgcagggt acgccgacgg ttatccgcgc cacgcgccta
     1561 ccggtacccc tgttttagtg gacggcgtgc gcaccatgac ggtggggacc gtctcgatgg
     1621 atatgctggc ggtcgattta acgccttgcc cgcaggcggg gattggtacg ccggttgagc
     1681 tgtggggcaa ggagatcaaa attgatgatg tcgccgccgc tgccggaacg gtgggctatg
     1741 agttgatgtg cgcgctggcg ttacgcgtcc cggttgtgac agtgtaactt gttgtaagcc
     1801 ggatcggaga cagcgtcttc tggttgcgaa aaaatcatcc atccggctgg tcagcaactg
     1861 tagttgttaa tgtgacagag ccattgccca tgatagtgtc cattaaaaga atggacacta
     1921 tttccccgga acctgaactc accgcacagg cgttctacat aaaacgctta cgcttcattg
     1981 ttgactcgaa ctcgacttca gataaatcac gctttaccct tgatggagcc tgtacataga
     2041 tttgtgtaat tgcctgattt tgatatgttc aattcaacat caaatgaagg ttaaattatg
     2101 gacgacaaac aattgcaggc tcaggctgcg ttcagcaaag catcgcaacc ggcgatagat
     2161 gcttcattaa atttaagatt cagcttcctc ttcagccacc cgtacgccaa tcttcaacac
     2221 ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta cctggtcacc
     2281 gacaaccggt gccgcgccta acaactgctg gacaatttca cccagcgttt gctgcttatc
     2341 acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat atttggcgct
     2401 ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggcg gcgactggct
     2461 gaacagttta ccgagcgccg gaagatcgcg ttcccgacca attacacaca acacatcgcc
     2521 ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa acagtgccgc
     2581 aatacgcgtc tcttttggca tatgcaaatc gcgcagtgcc gcgcccacgc accatttatc
     2641 ggcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat ccaggccaac
     2701 gcgtgacacc ggacgtccca ctggcggaac caccactttg gcttttttag ccgcccacga
     2761 gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga cattaaagaa
     2821 cagacgtgca ttctccagcc ccgccatcat cgggaacacg gccaggatga ttggcaccgc
     2881 gccgcgtaat cctacccagc tgataaacac gcgctcacgc agattgaaac cgcggaaggg
     2941 gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg ccgacaaaat
     3001 gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc ccagcaccag
     3061 gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga tgccgtagcg
     3121 gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca gaataccgct
     3181 gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg ccagcaatgg
     3241 atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat aaccaccgcc
     3301 aagtccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga acatccaact
     3361 gacactgctt tcatgttgct ggatcatcgc aatcagggta atcgtcagaa agaccgccat
     3421 tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt taagcccctt
     3481 accacccagc agagaaaaga ccgctgcagc atcggtagag ccgacgatag cgccgattaa
     3541 taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg ttaaaccaga
     3601 ggtgataagc acgcccagcg tcgccagcga cagtgccggt cctaacgcca cacgaaagga
     3661 actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagtgcca ggttacttac
     3721 catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga ctcctgccag
     3781 catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag aaaatgaact
     3841 aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa ttattgttgt
     3901 ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc taccatatta
     3961 gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt ttaagaacag
     4021 gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg gcatgtgcgc
     4081 ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta atgagtggaa
     4141 tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct gcgtcataat
     4201 cattgggcgt gctgccgcta aagctaccga gaataatcgc cttctgacgt ggcaaaattc
     4261 ccgcatgata gagctgcaac agcatacgtt cgacgcggaa agggtgctcg ttaatatctt
     4321 ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccaatc agtgaaatca
     4381 gcatcgcaag attgcctccc cacaacgtgc cttcggtctg acatgtcggg ccttcccctt
     4441 gccattcaat ggtgaaggtt ttattgcgta acgccagcca gaaatggtgc tcggtaaagg
     4501 cgttcagttc atccgcacca aagtttgcta ccagcatggg gccgctaaag gtgatgacat
     4561 tgccttgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgtccg caaatgagta
     4621 acgggtcatg ttgttggcga gcaaccagtg cctgccagtc aatatctgcc agtaaacgac
     4681 tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtagtc agtctggcaa
     4741 gggaattgag atcttccagg cgctccgttt ccgtacccgc aaaacgctca caacgacggg
     4801 caataacctc gacgttattg acctgatgcc cctcgtcggt aaggcgatgg ataccacgca
     4861 gcgcggcgtg ctgtttaatg cagtaacccg atggggcaat taagtgaaac agagacatgg
     4921 caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg tgccgttgtc
     4981 acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat ggtttgcctt
     5041 tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc cgccgtggaa
     5101 cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga aagccggtgc
     5161 agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat cgggtggtaa
     5221 tccgaacgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag cttcaacctc
     5281 cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca gcgagctgaa
     5341 aaatccagag cgtaatattt caatgggggc ggcttacctg aatattctgg aaaccggccc
     5401 gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt catacgctaa
     5461 cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga tcagcaaaat
     5521 caacgatttg gatgctgacg agttcctcga tcacgtagcg cgaaatcatc ctgcgccgca
     5581 ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa tcagtcgcgc
     5641 actttgtccg ctttttctcg ggcttctcgc tcgagagaga aaataatccg ctgtaattgc
     5701 cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt ggtgatggtt
     5761 tcattcttgc catcaatcac tttgcgctcg ctgatggaga ttaactgggc gtcaaagtga
     5821 aaaacacccc attgccccat gttgacttca atctgagcga agcgcatgcc ttcttgtaat
     5881 tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa atcatacagg
     5941 cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg cggatggagt
     6001 ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg aggcggtacg
     6061 gtgataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac agtaaactcg
     6121 actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag cacggcgatg
     6181 ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat tgccagtatt
     6241 ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc tttgtgcaaa
     6301 tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc atggtaatga
     6361 ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca cagttaacaa
     6421 aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc cgacatacgc
     6481 ccagcatcga tcccgccctt gcctatactt agagcgttga tgtaagcatt tcttgcgtcc
     6541 gattcatcga aacgagggcc tgaacatggg aattattgcc tggattattt ttggcctgat
     6601 agccggcatt atcgccaagc taatcatgcc ggggcgtgat ggtggtggat ttttcctgac
     6661 ctgtattctc gggatagtcg gtgcggtggt cggcggctgg ctggcgacca tgtttggcat
     6721 tggcggctcc atcagtggtt ttaatctgca cagcttcctg gtggcggtgg tgggagctat
     6781 tctcgttctg ggcatattcc gcctcctgcg aagagaataa gattttcata aggcggatag
     6841 cgatacagat gccgctatcc gctttcacat cagaacgtat actcgacacc tgctttacgg
     6901 gtgaaaaaaa tcaatatagc acttcgatat ccgataaagt cggttcccgg taacaacaac
     6961 agaatccttt tttaattatt gtttcgttgt ttttggtgtg attcagaaaa taaatatttt
     7021 agacagtagc cagtcaggcg ttaaaaatgg ggcggatagt gaggcggttt tggctgcgct
     7081 tattgaacat ttagaattga tcaatccttc tggcagatta agccaaaata cccgttcggc
     7141 aatgcttcag ttacgcgaag aggaatggtc tgaattcttt ttttggttgc taaattctct
     7201 ggagtgtctg gattatgtga ttattaattt aactccagaa agcaaaaaaa cgttgatgag
     7261 tgaacaccgt aacaatattc aggtagcaat tgatgcttta tatagccaac gtcggcgtaa
     7321 atcgccgggt gatgaaagtg aaacacttac ccgtagaaat gacgctatct tcggcaacca
     7381 cgtatggcaa acatttgcgc aatacttccc gccaggactt gaaaaaccgt ctgtgtagaa
     7441 aaaaccatag ctatggtgtt atctgccgac aacgccatcc ggagaagccg aatgacgttg
     7501 tcggagtaag cgctggttaa ggtgtgggtt gtgcctcttt ggttgagggt tgcgtcgttg
     7561 ctgacttaac ggtcggacgc gtcgccggaa cattgtcaca cggttgctct ttcgggcaga
     7621 tcaaatccag cattttcagc gtcacgccat tggtccagcc aaagccatcc tgtaatggat
     7681 attcgccacc gccgcccccc gttccggtgg tgctgacatc atatttttcc accagctttt
     7741 tctcccggtc ataggtgtgc tgaacattgg tcaggaagtg ccagctaatg tccatcgcca
     7801 cctctttttg cccgtagttt tgtaatcctt ctgtcgcgac ccactgtaac ggtgcccagc
     7861 catttggcgc atcccattgt tgcccacttt tcaccgacgt ggtgttcagg ccgccgggtt
     7921 gcagcagatg tgttttcgtc gccgtcgcca ttttgttggc gcgatctttc gctgccgcat
     7981 tgacgtacag cgggaacagg gcggccgcgg ttaactgatt gcgcacttta tgacttttca
     8041 ggtcgtaatc ggcataccag ccttgttgat cgttccacat gtatttttcg atcccttttt
     8101 gacgggcatt tgccagcgtt tcgtactggt ttgccatcgc gttatctccg gcagctttgc
     8161 tggcgcgggc gaggattttt tccattttaa acatcaggct gttcagatcg accggtacga
     8221 tgctggtggt gcgtaaggta tttagttgat gcgggttgtc catccagcgc gagctgaaat
     8281 cccagccaga cgcagcggca gagcgcaggt cgcggtaaat ttcagtggca ggtcgattcg
     8341 gattgctttt ggcggtggca atatcttcca cccatgactc tggtcgtggc gtatcgcgat
     8401 cgtcccagta gcggttgaga agggtaccat cctgaagttt tacaacgcgt ttttcctgtt
     8461 gtccggcttg caggttttca acaccgtcca tccagtaagc atattctttt tgcatttgcg
     8521 gcaggtattg cttcaacgcg gcatcgcctt catgctgcgc cagtaactct accatcaggg
     8581 caaagaaggg cggttgcgag cggcttaaat agtaactgcg gttgccgttg ggaatatgac
     8641 cgtaagtgtc tatttcatga gcaaaattgg ccaccatatc cgcgacttta tcccagtgac
     8701 cgctttcggc aagtcctaac atggtgaagt aactgtccca gtaatatacc tcgcgaaaac
     8761 gtccgcccgg cacgacataa ggttccggca gcggtaacag agaatcccat ttttcggtgt
     8821 tttcggtaga acgcgttaat accggccaaa gtccgtcaat atgttcgcgc agtgactgcc
     8881 cctctggcgg aacatatttc tcgccttctt tcggcagggt gaaattgacg ttaacgaaat
     8941 ggcgcagatc aaatccgctc tggttttgct gcatccgata atcagcaagg atcatcagcg
     9001 gatcgctgtt cggcacggca tcggcaaagg ttttttggtc cggaaaaagt ttggcgtttt
     9061 gcacatcatt aaacagcggc cctaataaaa tatcaggcgg ctgtggtgtt accggtgttt
     9121 cttctgcctg caccgatagc gcagcgaaac acaaaaagat acaggctgga attaacgcca
     9181 ttttttgcgg gcgagaaggt gcgggggatt tcatcaatca ttctcctttg gcgaaaccga
     9241 ataaaacggt attcaagtat cagaaaacct tagttcagga tcgcactagc tgcatgatcg
     9301 aaatctcatt ctgcgaactg tcaggcattt tagaaaaggg aattaaaggg ataaataacg
     9361 gcgaaataga gtgatcaaat aacgtctgtt tattagccag ccagcggcgt tgggttcgtg
     9421 attagcgaag aaaaatcatc ataaattggt gaaaaatata acaggtagtt aaaaaccatt
     9481 agtgctgagt aaattgccgg atgacatcag aacgatgcca tccgaacagt ggcttaaccc
     9541 tgacggttga aacgttgcct tttaacgtcc agcgttagcg tttcttctgg ttgtatcgca
     9601 tacagtttct caccctgctg gcaaatccag ccaatcccca gttcacgggc gattagggcg
     9661 ctgtgggata ccggacttcc ggcgctaagg cagatacctt ttacaaccgc cggatccagt
     9721 tgcagtactg tggaaggata aatgttctcc gccagtagaa tagttggcga gttaaactgc
     9781 gggagttctt ctttcgtttg ggtcaggtgg accagggtgc gatgcagaag atcgtccaca
     9841 tcaatatagc gagcttgtag atattcatca tccagttgct ggtattgctg gctaagttct
     9901 ttaagaactt gctgccaggc atattctgcc gtgcaatgtt catgctgaag gagttcgctt
     9961 gccgccgcca gcagttccgg atcatctaac agtgtatggt gaccagaaaa gattgcggca
    10021 atatcgtcaa gcccgctggc ttctgctttt gctgttaacg tcatcagatc taataacgtg
    10081 aagtcaatag cctggcgtaa tcgatcttgt tcttcttcca cggtcagggt tgattttgcc
    10141 tgtaccgtac ataaaactgg ttgataataa aaggctttac ccgaaacagg cggaacggga
    10201 cgcagagtag gtggagcgac ttcctccgtt tcaccaaagt tatcttcagc cagctgacgg
    10261 aaagcgatca gtgcctcttc agcttctggc cctttcgcaa tcaggcgcag cgtatcgtta
    10321 tagcgaactt gtagtaacgc aatctggtta atactctctg gtgtgacgca tttgccgttt
    10381 ttttccagca acatatcggc attaaatgtc gataaggtat aaaccagccg gg
//
LOCUS       DAMZET010000149        10417 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.149, whole genome shotgun sequence.
ACCESSION   DAMZET010000149 DAMZET010000000
VERSION     DAMZET010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.149"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..565
                     /gene="evgA"
                     /locus_tag="POB34_003747"
     CDS             <1..565
                     /gene="evgA"
                     /locus_tag="POB34_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HDI5920617.1"
                     /translation="NLLIKNDIEILAELTEGGSAVQRVETLKPDIVIIDVDIPGVNGI
                     QVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSKKEGMNNIIAAIEAAKNG
                     YCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKDNNDIAEKMFISNKTVST
                     YKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            570..4163
                     /gene="evgS"
                     /locus_tag="POB34_003748"
     CDS             570..4163
                     /gene="evgS"
                     /locus_tag="POB34_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HDI5920618.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKFIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(4219..5364)
                     /gene="yfdE"
                     /locus_tag="POB34_003749"
     CDS             complement(4219..5364)
                     /gene="yfdE"
                     /locus_tag="POB34_003749"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HDI5920619.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAAMLDQHGEQIRQEFSS"
     gene            complement(5438..6382)
                     /gene="yfdV"
                     /locus_tag="POB34_003750"
     CDS             complement(5438..6382)
                     /gene="yfdV"
                     /locus_tag="POB34_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HDI5920620.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6452..8146)
                     /gene="oxc"
                     /locus_tag="POB34_003751"
     CDS             complement(6452..8146)
                     /gene="oxc"
                     /locus_tag="POB34_003751"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HDI5920621.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8200..9450)
                     /gene="frc"
                     /locus_tag="POB34_003752"
     CDS             complement(8200..9450)
                     /gene="frc"
                     /locus_tag="POB34_003752"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HDI5920622.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(9963..>10417)
                     /locus_tag="POB34_003753"
     CDS             complement(9963..>10417)
                     /locus_tag="POB34_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HDI5920623.1"
                     /translation="VARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNL
                     NDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQ
                     YLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         3058 a         2358 c         2146 g         2855 t
ORIGIN      
        1 taatttattg atcaaaaacg atattgaaat cttagcggag ttgactgaag ggggaagcgc
       61 cgttcagcgg gtggaaacac ttaagcctga tatcgtcatc attgatgtcg atatccccgg
      121 agttaacggt atccaggtgt tagaaacgct gaggaagcgc caatatagcg gaattattat
      181 tattgtctcc gctaaaaatg accattttta cgggaaacat tgtgctgatg ctggcgctaa
      241 tggattcgtg agtaaaaaag aaggcatgaa caatatcatt gcggctattg aagctgcaaa
      301 aaatggctac tgctatttcc ccttctctct caaccggttt gttggaagtt taacgtccga
      361 ccagcaaaaa ctcgactcct tatcgaaaca agaaattagt gtcatgcggt atattcttga
      421 tggcaaggat aataatgaca ttgctgaaaa aatgttcatc agcaacaaaa ctgtcagcac
      481 ttataaaagt cgcctgatgg aaaaattaga atgtaaatca ctgatggatc tttacacatt
      541 cgcgcaacgt aacaaaatcg gctaatcaca tgaagttttt accctatttt tttcttctct
      601 gttgtggtct ttggtcgacc ataagtttcg cagacgaaga ttacatcgaa tatcgtggca
      661 tcagtagtaa caaccgtgtc acacttgatc cagtacgtct gagcaataag gaattacgtt
      721 ggttagcgag caaaaaaaat cttgtgattg cagtacacaa gtcccaaacg gctaccttgt
      781 tgcataccga ttcgcagcaa caggttcgtg gtattaatgc tgattattta aatcttttaa
      841 aaagagcgtt aaatatcaaa ttaacactcc gggaatacgc agatcatcaa aaagcaatgg
      901 acgcgcttgc agaaggtgaa gtcgatatag tgttatcaca tttagttact tcgccgcctc
      961 ttaataatga cattgctgca accaaaccat tgataattac ctttccggcg ctggtaacca
     1021 cccttcacga ctcaatgcga ccgcttacct caccaaaacc agtaaatatt gctcgggtag
     1081 caaattaccc cccagacgag gtaattcatc aatcatttcc aaaagcaaca attatctctt
     1141 ttacaaattt atatcaggca ttagcatccg tctcagctgg gcacaatgat tactttattg
     1201 gtagtaacat cattaccagc agtatgattt cccgctattt cactcactcc ttaaatgtag
     1261 tgaaatatta taactcgccg cgtcaatata attttttctt gaccagaaaa gaatctgtca
     1321 ttcttaatga agtactcaat agatttgttg atgctttaac aaatgaagtt cgctatgaag
     1381 tatcacaaaa ttggcttgat acaggaaacc tggcctttct gaacaaacca ttagaactca
     1441 ctgaacatga aaaacagtgg attaagcagc atcccaattt aaaggtgctg gaaaatcctt
     1501 actcgccccc ctattctatg acggatgaaa atggctcggt tcggggcgtt atgggggaca
     1561 ttcttaatat tattaccttg caaacaggtt taaatttttc tccgatcacc gtttcacaca
     1621 atatccatgc tggaacacag cttagccccg gaggatggga tataatacct ggcgctattt
     1681 atagtgaaga tcgagaaaat aatgttttat ttgctgaagc cttcataaca acgccttacg
     1741 tttttgtcat gcaaaaagcg cctgacagtg aacaaacatt aaaaaaagga atgaaagttg
     1801 ccattccata ttattatgag ctgcattcgc aattaaaaga gatgtatccg gaggttgaat
     1861 ggatacaggt cgataatgcc agcgctgcat ttcacaaggt taaggaaggt gaacttgatg
     1921 ctctggtcgc gacacagtta aattcgcgtt acatgatcga tcattactat cctaatgaac
     1981 tttatcattt tcttattcct ggcgttccga atgcatcgct ttcgttcgct tttcctcgcg
     2041 gagaaccgga acttaaggat attattaata aagcactgaa tgcaattccc ccaagcgaag
     2101 ttctgcgcct gacggaaaaa tggattaaaa tgcccaatgt gaccattgac acatgggacc
     2161 tatatagcga gcaattttat attgttacga cattatccgt tttattagtt ggcagtagcc
     2221 ttttatgggg attctacctg ttacgctcag ttcgtcgtcg taaagtcatt cagggtgatt
     2281 tagaaaacca aatatcattc cgaaaagcac tctcggattc cttaccgaat ccaacttatg
     2341 ttgtaaactg gcaaggtaat gtcattagtc ataatagtgc ttttgaacat tatttcactg
     2401 cggattacta caaaaatgca atgttaccat tagaaaacag tgactcaccc tttaaagatg
     2461 ttttttctaa tgcgcatgaa gtcacagcag aaacgaaaga aaatcgaaca atatacacac
     2521 aggtatttga aattgataat ggcatcgaga aaagatgcat taatcactgg catacattat
     2581 gcaatcttcc tgcaagtgac aatgcagtat atatttgtgg ttggcaagat attactgaaa
     2641 cgcgtgatct aattaatgca ctcgaggtag aaaaaaataa agcgataaag gctaccgtag
     2701 caaaaagtca gtttctggca acgatgagtc acgaaataag aacaccaata agctctatta
     2761 tgggcttcct ggaacttctg tcgggttctg gtcttagcaa ggagcaacgg gtggaggcga
     2821 tttcacttgc ctacgccacc ggacaatcac tcctcggctt aattggtgaa atccttgatg
     2881 tcgacaaaat tgaatcgggt aactatcaac ttcaaccaca atgggtcgat atccctactt
     2941 tagtccagaa cacttgtcac tctttcggtg cgattgctgc aagcaaattc atcgcattaa
     3001 gttgcagcag tacatttcct gatcattacc tggttaagat cgatcctcag gcgtttaagc
     3061 aggtcttatc aaatttactg agcaatgctc tcaaatttac caccgagggg gcagtaaaaa
     3121 ttacgacctc cctggttcac attgatgaca accacgctgt tatcaaaatg acgattatgg
     3181 attctggaag tggattatcg caggaagaac aacaacaact ctttaaacgc tacagccaaa
     3241 caagtgcagg tcgccagcaa acaggatctg gcttaggctt aatgatctgc aaagaattaa
     3301 ttaaaaatat gcagggcgat ttgtcattag aaagtcatcc aggcatagga acaacattta
     3361 cgatcacaat cccggtagaa attagccagc aagtggcgac tgtcgaggca aaagcagaac
     3421 accccatcac actacctgaa aagttgagca tattaatcgc ggatgatcat ccgaccaaca
     3481 ggctattact caaacgccag ctaaatctat taggatatga tgttgatgaa gccactgatg
     3541 gtgtgcaagc gctacacaaa gtcagtatgc aacattatga tctgcttatt actgacgtta
     3601 atatgccgaa tatggatggt tttgagttga ctcgcaaact ccgtgagcaa aattcttcct
     3661 tacccatctg ggggcttaca gccaacgcac aggctaacga acgtgaaaaa gggttaagtt
     3721 gcggcatgaa cttatgtttg ttcaaaccgt tgaccctgga tgtactgaaa acacatttaa
     3781 gtcagttaca ccaggttgcg catattgcac ctcagtatcg ccaccttgat atcgaagccc
     3841 tgaaaaataa tacggcgaac gatctacaac tgatgcagga gattctcatg actttccagc
     3901 atgaaacgca taaagatcta cccgctgcgt ttcaagcact agaagctggc gataacagaa
     3961 ctttccatca gtgtattcat cgcatccacg gtgcggctaa catcctgaat ttgcaaaagt
     4021 tgattaatat tagccatcag ttagaaataa cacctgtttc agatgacagt aagcctgaaa
     4081 ttcttcagtt gctgaactct gtaaaagaac acattgcaga gctggaccag gagattgctg
     4141 ttttctgtca gaaaaatgac taaatagcgg ctcccacaat gttcaaatgt gggagctatt
     4201 taccagcaca tcttacattt atgatgagaa ctcctggcga atttgttccc catgctggtc
     4261 gagcattgcc gctcccggca taacatgcgg gtccgcgcag ccgctgattt ttatcggatt
     4321 acccggcatc attattcccc cggcttcaat caacatattt cttgcctgag tttgtggcaa
     4381 tttaatggcc tcagccacac ttaataacgg cgcgacgggt acaccaactt catgtattct
     4441 ggctaaccaa acttcagctg cctgcgtttt taacgtccgc tcaatatatt gtttaagaat
     4501 agcctggttt tgtacgcgta aaatattgct gctaaatcgg ggatcattaa ccagttccgt
     4561 aagctccagt gcctggcata acgcagaaaa aagcttgtca ttaccacaac aaatcgtaat
     4621 cggcttatcc tgagtattga aaacatcaaa aggtgccatg tagggatggc gatttcccag
     4681 acgttgtggt gacttccctg tcgcgatata tgccatcaga ccatgctcca gaaaactcag
     4741 cgtggcatca aacatcgcta tatcgacatg cgcccctctc tggctctttt cgcggccata
     4801 aagtgcactc actattccgc tgaataaata gacaccgccg catagatccg caagagatgt
     4861 accaacgcgc actggcggag catcaggata tcctgtttcc atcattatcc cgctcattgc
     4921 ctgaatgatg gtatcgtagg caggagcatc ttttagcgga ccggtatgtc cgaaacctga
     4981 cgatgaagca tatatgaggc gcgggttgat ttcttgaagc gtttcccatg aaaaccccag
     5041 tttttccatt gtacctgggc gaaaattctc agctaataca tcagcttgtt tgagcatatt
     5101 tataaatata cttttatcgt gatcattctt taaatcaaga accacactct ctttgccatg
     5161 attaataaaa ctgtaataga gtgactgtcc atccacatag ggaccaaatg tgcgggtatc
     5221 atcaccatga cccggcggct caactttaat tacccttgcg cccatattac aaagaagttg
     5281 agttccgaaa ggtccattaa ggacatgtgt catatcgata actaataagc cttcaaacgg
     5341 ccctttgctt tcattatttg tcatttgctt atgccttgaa aaaagccgaa ggtgaaaaga
     5401 cacttcggca tttatgtaaa atcaataaca tgattaatta tgaaaccagt cgactgacat
     5461 aaatccacaa gggagccgtg acgacaaagc ccagtacgct cacagccaat gacgctgtac
     5521 cagtacgggt gtagacatta aaccggctgg caataatgat tccggagaat gccggcggta
     5581 atgcgcctgc cagtaccatc atctgcagat gttcgctgtt caaatgacat gccataccaa
     5641 cgagaagcag tgccagtggc atcagaatca gcttcaggaa ggtgttataa gcaatttcgg
     5701 cactgaactc gaatttatgt gcagccagag tcaaccccgc agcgaatacc gctacccctg
     5761 agttagcctt cgcaatcaga ttaaaggttg ggtcccatgc tgccggaatt tttaccccaa
     5821 ccaacaccag gatcgttgcc agaacaggtg cccatactac tggctccttt gccgcagaaa
     5881 ttaatgcgct cagattacta ttcttcttac catccgctcc tgaggaagga ttcagcaaat
     5941 acagaccaat aggaatagta attgcgttaa caataataga aataattgct accactaaac
     6001 ctgttgatac ggaatcacca taaataggat cgagaactgc aaaccccagg aatccaatgg
     6061 taggtgaacc cgcaattaat gcacatacag ctgcttctgc atgggtacgt ttaaaaaatt
     6121 tgtagcaacc aaaccaggag aaaaagaaac atccgacaat aaccacaagt gatacaaggg
     6181 tcagacgagt gtccgcaaaa atcatttccc tgtttgcccg ggtaatagaa acaaatagag
     6241 ccgcaggaag agcgtagttt aataccagtt tattaaatgc gcgggcttga tcttctgaaa
     6301 aagtttctcg tctgccgcta aaataaccca acagcatgat tacgataata ggcaataaat
     6361 cgccaataaa aaatgttagc ataatagtat ttccgataag ggtcccctca ccatcgcata
     6421 atgagttggc aagaggagac gatgtcagta tttaattacc agcgacttgt tttgggttaa
     6481 gtttggtaat atggccactt tcagttcctg ctgcagggtc gatgaccaca ttaataatgg
     6541 tcggtttgcg cgactgaata ccggtggtta aagcatgacg aagttcatct gtcgtggtga
     6601 cgttatagcc aacgccacga aacgcatcca ttaatttgtc ataccttgca tggtgcaaca
     6661 gatccgttgg tgatggtgca ccagcgccac tgagatcaac accgtctcct ctgtagatgc
     6721 cgccattatt aaaaataacg atcgtcaccg gcaggttata tcgacaaatc gtttcaattt
     6781 ccatcccact gaaaccaaaa gcactatcac cttcaatggc gacaaccgga gaaccagaag
     6841 tcacgctagc accgatggca tagcccatac cgatgcccat gacaccccag gtaccacaat
     6901 ccagacgacg acgtggttta tacatatcaa taatatttcg tgcattatcc agggtatttg
     6961 caccttcatt aactaaataa atatcctggt tctcgcgcaa tacgtcgcgc acagcactta
     7021 atgcattaaa gtaatttaat ggttgagtat ctgtacttaa tttttcatgc attttttgtg
     7081 cattttgctg cttgtggata tttaaaatat cgcgccatac cagtggagtc gtaaatgtgt
     7141 tttgtttcag ttctgccagc ataccttgca tactggatgc aatatcacca acgactggca
     7201 cagcaatggg gcggttgctg tcaatttcct gcggttcaat atccagttga ataaactgtg
     7261 tatctgccgc ccatcctttt ttaccgtgtg ccaataacca attcagtcgt gcaccaacaa
     7321 gcatgacaac gtcagcattt gccagggcaa acgaacgcgc agctgccgca gaaagtggat
     7381 gcgtatcttc aaggatccct ttcgccatag acattggcag gaatggaatc tgagtacttt
     7441 caataaattc acgaagctgt tcatcagctt gtgaatacgc cgcgcctttg ccaaggataa
     7501 ttaatggccg ttcagctttt gctaaaagcg aaattgcgct agtgactgac ttcgggcatg
     7561 gcaataatgc tggcgacgga ttttcaactt taacaatcgt ggttaacgct tcgtcttttt
     7621 ccatcgtcgc ggccaggaca tttgctggca aatcaagata aactccgcca gggcgacccg
     7681 atacagagac ccggatagcg cgtgccaatg caatgccaag atcctgcggc tgattaacgc
     7741 gaaatgctgc tttggcatac ggttttgccg cattcatttg gtccagctct tcataatcac
     7801 cttgctgtag gtcgacgatc gcgcggtcgc tggagccgct aatcatgatc atcggaaaac
     7861 catttaccgt tgcgttggcc aatgcggtca aaccattgag gaatcctggc gcagaaactg
     7921 tcaggcagat ccccggtttt tgggtaagaa aaccgcttgc cgcagcggca tagcctgccg
     7981 actgctcatg acgaaaacca atataacgaa tgccttccgc ctgggcatgg cgtgccatat
     8041 ccgtcacagg aatacctaca acaccataaa tagtgtcaat attattctgt tttaatgctt
     8101 caacgatgat atgcatacca tctgtcattt gaagttgatc tgacatgatt aataacctcg
     8161 aaatttttat aaattgggag ccagaagccc ccgttaatat cagatggcgt ggttttgctt
     8221 cattgcagca atttcatcgt cactataacc cagctcctgc aatacagcag cggtatgttc
     8281 acctaatagc ggcgcagctt taatatccgg cgtaaaggca gagaatttca ttggacaacc
     8341 aacggtcaga tattttccac gcaacggttg ttccacttca acaacactgc cactttggcg
     8401 caaagaggga tcaagtgaaa tttctttcat acttaaaacc ggtgcacaag gaatatcaaa
     8461 ctgagtcaaa taggccaccg cttcatgttt atcaatagtg acagtgtatt tttcgatttc
     8521 agcaaaaata tcgaaaatat gtggctgtcg tgcatgggct gtactgtatg ccggatcggt
     8581 aatccattct ggtttgccga tggctttaca ggtgttttcc cagttttgct cctgaatagt
     8641 gaaataaata taggcgttag gatcggtttc ccagccttta catttcagga tccagccagg
     8701 ctgaccgcca ccacctgcat taccaccgcg gggaactgca tcaccaaatg taccattcgg
     8761 atactgcggg tattcttcca gataacccaa tttatcgaga cgctgctggt cacgtaattt
     8821 cacgcggcaa aggttcaata cggcatcctg cattgacatg gtgactcgtt gcccacgccc
     8881 cgttttttcg cgatgcagca aagcagcaag taaaccgatc agcaaatgca ttccggtgtt
     8941 gctgtcaccc aacgctgcag cgcttaccag cggcggacca tcccaaaaac ccgtagtgga
     9001 tgccgcgcca cccgctgcct gagcaacgtt ttcataggct tttacattca cataaggcga
     9061 acactcatca aaccctttga tcgaaccaaa aatcagacgt ggattgattt cttgaatatg
     9121 ctcccaggtg aagcccatgt gatcaatggc ccctggatga aagttctcga ctaagatatc
     9181 agcttcgcgg atcagctttt ccattacctc tttgccttcc gctgttttgg tatttaactc
     9241 aatagaacgt ttgttactgt taagcatggt gaagtaaagc gcatcgatat caggaatatc
     9301 tcgcagctgg tgacgcgtta cgtcaccaac gccgggacgt tcaattttaa taacgtcagc
     9361 gccaaaccag gccagcattt gagtacaaga tgggccagat tgcacaccgg tgaaatcgag
     9421 aactttaatt ccttgaagtg gagttgacat agcaatacct attagttaat taaatatgtg
     9481 ttaatttagc gtttatttaa ggcgtaatat cttctataaa taacccgcaa aatatcgtcg
     9541 tagctgcact cgatattttt aaaatcctgt atcaagtgct gaaaatcatt tcatcgccac
     9601 ttccaattcc tttctgcatg gtaagtagtg aggacgaaaa gaagaatatg ataaatattg
     9661 attcgtggta atcagagaga gtcgcgacat ttgtgatgtt attcttacaa ctgtaagaag
     9721 cgccggtgat ttacaagtaa gggtatgatt acgcttttta aagcgtacca taaaacataa
     9781 cataataaat gatttgtgat gacacgggat tattgggatt tactttgagg aagataaaat
     9841 gcgtgtttat agaaccgcga gcgccaaata attttatgac cacaacctgt cgtattgtgg
     9901 tcataaaaaa ttgtcggctg gagggagtta aatcctttac ctcaatatac tgcctgtcag
     9961 cattagttca caaacagtgc ttcgctgtcg acgatgattc catcttccgc acctttaagc
    10021 gccaggtttg cctcgtagta ttgcttttta tcaagcagtt tctgcgcatc agcaagtgca
    10081 ttacgagtct gtttaagcgg catcagatat tggttttcca ttacgccaac accggccagg
    10141 cgaagttctt ccattgcgcc ctttttatcc cctttcgcca ttttttcatt ggcaattttt
    10201 atcgcggctt ctttttcggg agttgctaca taactctcag aaattccgac agatgcatta
    10261 atgacaatat attggtcatc attgagattg gtctttttac ctggcttagc gaactttgcc
    10321 cattcggtac tgtcatctga cagcaaagcg gaagcttcat tggtcagttc ttttgccttt
    10381 tctggatcgc catgaaacag agccaggcgc gctacct
//
LOCUS       DAMZET010000150        10153 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.150, whole genome shotgun sequence.
ACCESSION   DAMZET010000150 DAMZET010000000
VERSION     DAMZET010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.150"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..455)
                     /locus_tag="POB34_003754"
     CDS             complement(<1..455)
                     /locus_tag="POB34_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HDI5920624.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFG"
     gene            722..2221
                     /gene="ansP"
                     /locus_tag="POB34_003755"
     CDS             722..2221
                     /gene="ansP"
                     /locus_tag="POB34_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HDI5920625.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2336..3397)
                     /locus_tag="POB34_003756"
     CDS             complement(2336..3397)
                     /locus_tag="POB34_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDI5920626.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3639..5741
                     /locus_tag="POB34_003757"
     CDS             3639..5741
                     /locus_tag="POB34_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDI5920627.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTSVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNLGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(5777..6442)
                     /gene="mcbR"
                     /locus_tag="POB34_003758"
     CDS             complement(5777..6442)
                     /gene="mcbR"
                     /locus_tag="POB34_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HDI5920628.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6640..7677)
                     /gene="curA"
                     /locus_tag="POB34_003759"
     CDS             complement(6640..7677)
                     /gene="curA"
                     /locus_tag="POB34_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HDI5920629.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPNYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            7858..8376
                     /gene="mddA"
                     /locus_tag="POB34_003760"
     CDS             7858..8376
                     /gene="mddA"
                     /locus_tag="POB34_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HDI5920630.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8373..8822
                     /locus_tag="POB34_003761"
     CDS             8373..8822
                     /locus_tag="POB34_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDI5920631.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(8823..9056)
                     /locus_tag="POB34_003762"
     CDS             complement(8823..9056)
                     /locus_tag="POB34_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HDI5920632.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9142..9315)
                     /gene="ortT"
                     /locus_tag="POB34_003763"
     CDS             complement(9142..9315)
                     /gene="ortT"
                     /locus_tag="POB34_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HDI5920633.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFMVGATW
                     PMSFPVALLFSLF"
     gene            9510..9605
                     /gene="yncL"
                     /locus_tag="POB34_003764"
     CDS             9510..9605
                     /gene="yncL"
                     /locus_tag="POB34_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HDI5920634.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(9702..>10153)
                     /locus_tag="POB34_003765"
     CDS             complement(9702..>10153)
                     /locus_tag="POB34_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDI5920635.1"
                     /translation="AHLERVSKAVEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGAL
                     QDDAIVQKEVFGPVVSVTLFDNEEQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQY
                     GCTWVNTHFMLVSEMPHGGQKLSGYGKDMSLYGLEDYTVVRHVMVKH"
BASE COUNT         2422 a         2516 c         2494 g         2721 t
ORIGIN      
        1 ccaaacgcat aaggttcagc ggtcaattgt gaattcagcc agatataaag cgatttacga
       61 tattcaataa cattcttctt taactgttca ggtgcgtcag gagcccaacg ctcagggtaa
      121 tcggcgaaag tgaatgttgg atagacatta gcaaccagcc agaccaatag ccgttgaaac
      181 tgctgacgtt cggcgcgccc aaccggtggc gcaaggtccg ggcgacgatc gagaaccatc
      241 aatgcaatcg ccgctgtctc cgtcataatc tcgtcatttt ccagcgccag tgtcggaatc
      301 tgacacaacg ggttcagggt tttcaataac tcgcgtgaag ttccctcgtg gtcaaaacca
      361 ctgacatcaa cgaactgata aggaatatca gccagtgtta gcatcagctc actgattgta
      421 gagccccatc cgggtacgcc atacacctta atcatatgcc cctcacaatc ttttaaagat
      481 taagtgtagg acagtacaca ggttaaccag caaaaggatt tgtcattttt tcgtcagttg
      541 tattaatccc cttcaggatt ataaggggat gttttgcatc gtttatcgat aacgggtagg
      601 atgctgcgcc gatagtttta tacccataaa gaataatggt gataactatc atcgccagga
      661 tgaataaaca ttgttcatgg caacttatat gactttttca ttaaagcaat cagggagagc
      721 aatgagtaaa cacgacaccg acacttcaga tcaacacgcc gcgaaacgcc gctggcttaa
      781 tgcccacgaa gaggggtatc acaaagcgat gggcaatcgc caggtacaga tgatcgccat
      841 tggcggcgcg attggcaccg gcttgttttt aggtgcagga gcccgactgc aaatggcggg
      901 tccagcactg gcactggttt atttaatttg tggcttgttt tcgtttttta ttctgcgtgc
      961 attgggtgag ctggtgctac accgcccttc cagtggcagt tttgtctctt atgcccgtga
     1021 gtttttgggt gagaaagccg cttatgttgc tggctggatg tacttcatca actgggcgat
     1081 gacggggatt gttgatatta ccgccgtcgc tctgtatatg cattactggg gtgcgtttgg
     1141 cggcgtgccg cagtgggtct ttgcgctcgc tgcacttacc atcgttggca ccatgaatat
     1201 gatcggtgtg aaatggtttg cggagatgga gttctggttt gcgcttatta aagtgctcgc
     1261 cattgtgacc tttttggtcg tgggtacagt gttcctcggt agtggtcagc cgctggatgg
     1321 caacaccact ggctttcatt taatcaccga taatggcggc ttcttccccc acggtttgct
     1381 gcctgcgctg gtgttgattc agggcgtagt gtttgctttt gcctccattg aaatggtggg
     1441 tacagctgcc ggagaatgta aagatccgca gaccatggtg cctaaagcga ttaacagcgt
     1501 gatttggcgt attggcctgt tttacgtcgg ctccgtggtg ttgctggtta tgctattgcc
     1561 gtggagcgcg tatcaggcgg ggcaaagtcc gttcgtgacg tttttctcta aactgggtgt
     1621 gccatatatc ggcagcatta tgaacattgt ggtgctgacc gctgccctct ccagcctgaa
     1681 ctcaggtctg tactgcaccg gacgtattct gcgctcaatg gcgatgggcg gttccgcacc
     1741 gagttttatg gcgaaaatga gtcgtcagca tgtgccgtat gccgggattc tagcaacact
     1801 agttgtgtat gtcgtcggcg tattcctcaa ctatctggtg ccgtcgcgcg tatttgagat
     1861 tgtgttgaac ttcgcgtcgc tgggaatcat cgcttcatgg gcgtttatca tcgtgtgcca
     1921 gatgcgcctg cgtaaagcga ttaaagaagg caaagcagcg gatgtcagtt ttaaactgcc
     1981 tggcgcgccc ttcacttcct ggctgacatt actgttttta ctgagtgtcc ttgtgctgat
     2041 ggcgttcgat tacccgaacg ggacttatac tatcgcggcg ctgccgatta tcggtattct
     2101 gctggttata ggctggtttg gtgtgcgcaa acgcgttgct gaaattcaca gcactgcgcc
     2161 agtcgtcgag gaagatgaag aaaaacagga aatcgtgttt aagcctgaaa cggcgagcta
     2221 attgcaaaat tgcccggcta cgtgccgggc aatttcaaat tactgacgaa ggtgtgccgc
     2281 atgcggcgtg ataaaagtca ggcaagtctc tccgttttca ccctcgccat atctattaca
     2341 gcgcaatacg aatcacatcg tccggctggg tagcttcctg ctgtttagtg gatttttgtt
     2401 ttacactgac atacagcgtt ttgccatcgg cagacagcgc caggctgttc ggatgagtcg
     2461 gcgtatcgaa cgttttcacc actttatagc ttttcgcgtc aatcacactg actttacctg
     2521 cctgacgatg cgttacgtag gcttcattac gcgctgggtt aaacagcaca gccagtgatt
     2581 ccggtgccgc aaccttcgcc agaatattgc cattacgggt atcgaccact aacacttctg
     2641 ccgctttaga atcggtgata aatgcacgct gcctggcggt atcaaggcta atgttgataa
     2701 agaagtgctc tttgccgtca tccagcagct ttttacggct gaggatttta ttgtcggcgg
     2761 tgtcgatggt aatcaattcg ccgtcagcgt tagtggtgta aagacgtttg cctttgctat
     2821 ccagcgccag accggtactc attttaccgg tgttctggat ggcggttttc agtttgatat
     2881 tctcgccatc aacgacccaa atcacgctct ctttaccaat accactgatg taaacggtgt
     2941 tcgtggcatc atcagctacc agctcacgcg gttgcagcgg gcgcacctct tccgtgcgct
     3001 tacgatcatc cagcaccaga cggcctttca cttcgcccgt tttggcatct atcgccgtga
     3061 ccgcgctgtt tacggtgtta ccaaaccaca acgtctgagt cgtgttattg atggtggcac
     3121 caaacggctt gagatcgtta tggatcgcct gcgtcacttc cagagtgacc ggatcaagac
     3181 gataaaccac gccaccttta tccagtttgc ggctttgcga agtggcgagc cacagcgcgt
     3241 tttcttgctg gctataagcc atttcgtagg cacctttacc taccgcttta cgcagcattt
     3301 cttctgcggc ctgcgtactg aatgatgaag caacaagcaa tgaacctaac agtaatgaac
     3361 cacgcaggcg cgatgaaaac agatgacgta aatgcatgac gactcccttt gatgaaccaa
     3421 tatttttgct gcttcacaat gcttcgctaa gcgtaatcat cgttacgctc ttgttataaa
     3481 tgggaatggt aatcattatt ttctcaaatg tggagcattt cttgccgtta cgactacaat
     3541 tttctcaatt aattaacgta tcgtgccgtt tcgttttcca taaatttaca attttattac
     3601 catgacagcg ttttcccatt cttaacctgg cttttttcat gaagattttt tccgtccgac
     3661 agaccgtttt gcccgcactg cttgtccttt cccccgttgt ttttgccgct gatgaacaga
     3721 ctatgattgt cagtgccgca ccgcaggtgg tttccgagct ggatacgcca gcagcagtaa
     3781 gcgtggtgga tggcgaggag atgcgcctgg caacaccgcg cattaacttg tccgaatcac
     3841 tgaccagcgt gcctggtttg caggtacaaa accggcagaa ctatgcgcaa gatttacagc
     3901 tgtcgattcg cggatttggc tcccgttcca cttacggaat tcgcggtatt cgcctgtatg
     3961 tggacggtat tcccgccacc atgcccgacg ggcaagggca aacatccaac atcgatttaa
     4021 gcagtgtgca aaatgtggaa gtgctgcgtg gccccttctc tgccctgtat ggcaacgcgt
     4081 ctggcggtgt aatgaatgtc accacccaga ccggacaaca gccaccaacc attgaagcca
     4141 gtagttacta cggcagtttt ggcagctggc gctatgggct gaaagcaacg ggcgcaacgg
     4201 gagacggtac acagcccggc gatgtcgatt acaccgtctc aaccacgcgt tttacgaccc
     4261 acggctatcg tgaccatagt ggcgcacaga aaaatttagc caatgccaaa ctgggcgtac
     4321 gcattgatga agccagcaaa ttaagcctga ttttcaatag tgtggatatc aaagcagatg
     4381 acccaggtgg gctaaccaaa gcagaatgga aggcgaatcc acaacaagcg cctcgtgcag
     4441 aacagtacga cacgcgaaaa accatcaagc aaactcaggc tgggttgcgc tatgagcgta
     4501 gcctgagcgc gcaagatgat atgagtgtga tgatgtatgc cggagagcga gaaacgaccc
     4561 agtaccagtc aatcccgatg gccccccaac ttaacccgtc acatgcgggc ggcgtgatta
     4621 ccctgcaacg ccattaccag gggatagaca gccgctggac acaccgtggt gaactgggcg
     4681 ttccggtcac gttcactacc ggcctgaact acgaaaacat gagtgaaaac cgcaagggct
     4741 acaataactt ccgcctgaat agcggcatgc cggagtacgg gcaaaaaggt gagttgcgtc
     4801 gcgacgaacg caatctgatg tggaacatcg atccctattt acagacgcag tggcagctga
     4861 gcgaaaaact gtcgctggat gctggcgtgc gctacagctc cgtgtggttt gattccaacg
     4921 accattacgt tactccgggt aacggcgatg acagcggtga tgccagttat cataaatggc
     4981 tacctgccgg ttcgttaaaa tatgcaatga ccgatgcctg gaatatctat ctggcagccg
     5041 ggcgaggttt tgaaacgccg acgattaatg agctgtctta tcgtgctgat gggcaaagcg
     5101 gtatgaactt aggtttaaaa ccatccacca acgatacaat tgagatcggc agtaaaacgc
     5161 gtattggtga tgggctgctt agtctcgcat tgtttcagac cgacactgat gatgaaattg
     5221 ttgtcgatag cagtagcggt gggcgtacga cttacaaaaa tgccggaaag acccgtcgtc
     5281 aaggcgctga actggcatgg gatcaacgtt tcgcaggaga ttttcgcgta aacgcgtcct
     5341 ggacctggct tgatgcgacc tatcgcagca atgtttgcaa tgaacaggat tgtaacggta
     5401 atcggatgcc agggatcgcc cgtaatatgg gctttgcgtc gataggttat gtaccggaag
     5461 atggttggta tgcaggcacg gaagcgcgtt atatgggcga tattatggca gatgatgaaa
     5521 atacggcaaa agcgccgtct tatactctcg tcggcttatt caccgggtat aaatacaatt
     5581 accacaattt aactgtggat ttatttggtc gtgtcgataa tttattcgat aaagaatacg
     5641 ttggttctgt cattgtcaat gagtcaaacg ggcgatatta cgaaccttcg cccggacgaa
     5701 attatggtgt cggcatgaat attgcgtgga gatttgagta attaccttga tgcccggtat
     5761 ttgccgggca tttactttaa cgattgtatt gctggtataa aatagcaata ttttgttgca
     5821 taatttcagc aagacaatgt ctgcatccct ctttgtcttt tgcttttaac gcagcgagta
     5881 acagatgata gttttctata tgctcccgta attccgctgg attaatcgct tcatagagat
     5941 aatgtaaacc aggccccatc ctgacccaca gttgctcaat catctcacac aggatgggca
     6001 tatttgagcg atgataaatc gctaagcgaa atagcctgtt tacattaatg atttgttcca
     6061 tgtcgccctt ttcttgcgcc tgctgtaatt tctcgagcaa ttcctggagt tccgcaaggt
     6121 cttgcggggt gaggttttca acagccagag caactgccat taattccagt tcgtagcgga
     6181 tccgattgat ttcgtctaat tgacgtttcc ccacttccgg aactgtaaat gcttgtgcag
     6241 gtgcgacaga aagcgcattc accgaaacca gacgtaataa tgcttcacgc acaggtgtaa
     6301 tactcatacc taattgctcc gccagatttt tagtaatcag gcgtgcgcca ggttttaatg
     6361 cgccaatact aagctgatgt ttcaggtcgt tctcaacctg cagagtcaaa ctgacatgtt
     6421 tcatttttcc cgttccaggc attttaattc cttcgcgtgt tttctgtcca accctgcctg
     6481 catggcagaa ggagttcacc tggtgtatta aagtgaataa tcataaacca cccacctttg
     6541 ttgttattag cagcagaaca atgacgtggt tagttaatta aagctgaaaa taagcttttt
     6601 gcctggattt ttatggcggc ttacgccgcc gttgaaactt taatcatcac ccgcgacgcg
     6661 gatcaccact ttgccgaagt ttttaccctt cagcaagccg ataaacgtct gtggcgcatt
     6721 ctctaaacca tcagtaatat cttcgtggta gtggatttta tcctctttca cccattgccc
     6781 catctccttc tgaaactcat ggatgcggtg accataatcc tgagcgataa taaacccttg
     6841 caagcgaata cgttttttca gcaccgttgc catcaacagg ggtaaacgat ccggaccggg
     6901 tggtagctct gtagcgttat agctgctcac taagccacaa acgggaatgc gcgcagatgt
     6961 attaagtaat ggtagcactg catcaaatac cttaccgccc acgttttcat aatagatatc
     7021 aatacctttt gggcacgctt tcaccagttg ttcggcaaaa tcatccgcgt ggtgatcaag
     7081 acaaacatcg aagcccagca cctcgatagc atggcggcat ttttccgcgc caccggcaac
     7141 ccccaccacc ctgcaacctt taagtttgcc gatttgcccc accgttgctc ccacaggacc
     7201 tgtcgccgca gctaccacca acgtttcgcc ctctttaggc tgaccgatat ccagcagccc
     7261 catataagcg gtaaagcctg gcatccccag cacacccagc gaccacgatg gattttgcgg
     7321 atgatcgcca agtttcacca gatcatcacc actggatatg tcatagtcct gccatccact
     7381 gtagcccagc acccagtcgc caggctgata attaggatga ttcgactcca caacacggct
     7441 caccgtgccg ccgaccatca cgccgccaat atcaacaggc ggtgaataag atggctcatc
     7501 gctcatacgt ccacgcatat acgggtccag ggacaaataa actgtgcgca gtaacacctg
     7561 tccttcaccc ggtgtggcga catcatcttc ttcaagacgg aaattctccg gaaccggtgc
     7621 gccatgtgga cgcgaggcca gaacccaacg tcgattacgc tgcttttgtt gccccataat
     7681 gctctcctgt gcttttggca tgaatccatc aatagtagca ggctcacggg attggttttg
     7741 tttgcagccg tcacatcccc tctgcaacac gcaataattt catcgaggaa tccgttgctt
     7801 cgggctgttt tcgatagtat tgataacatc atcatcatta ttttatcgag gccagacatg
     7861 tccatccgtt ttgcccgcaa agccgattgt gctgccattg cggaaattta taaccacgcc
     7921 gtgttgtata cggcggctat ctggaatgac caaacggttg atgcagataa ccgcattgcc
     7981 tggtttgaag cgcggaccat agcaggttat ccggtgctgg taagtgaaga agatggcgta
     8041 gtgacgggat atgcctcgtt tggcgactgg cgtagtttcg atggttttcg ccataccgtg
     8101 gaacattcgg tttatgtcca tcccgatcat cagggcaaag gtctggggcg taaattgtta
     8161 agccgattga ttgatgaagc gcgggattgc gggaagcatg tcatggtcgc cgggatcgaa
     8221 tcgcaaaatc aggcctcgct gcatctgcac cattcgctgg gatttgtcgt caccgcacaa
     8281 atgccgcagg tgggcactaa atttgggcgc tggctggatc tgacatttat gcagttgcaa
     8341 ctcgacgaac gcactgaacc ggacgcgatc ggatgaatca gtcgctcacc cttgcttttt
     8401 tgattgccgc cggaattggt ctggtagtcc agaacacgct gatggtgcgc atcacccaga
     8461 cctcctctac cattctcatc gccatgttgc tgaactcact ggtggggatt gtgctctttg
     8521 tttctatttt atggtttaaa caaggcatgg cggggtttgg cgaactggtg tccagcgtgc
     8581 gctggtggac acttataccc ggcttgctgg gatcgttttt tgtcttcgcc agtatcagcg
     8641 gatatcagaa tgtgggagcg gcgacaacta ttgcagtact ggtcgccagc cagctaattg
     8701 gcggtctgat gctggatatc ttccgcagtc atggcgtacc actacgggcg ttgttcggcc
     8761 caatttgtgg cgcaatttta cttgtggttg gcgcctggtt ggtagccaga cgctcatttt
     8821 aatcagagga tggtgccgcc tttggttaac tgttcgtggc gcgcttccat atcttcttta
     8881 tgcttgcgac cgtgatgagc aatagcggtg cgcaaacgct gctgctgggt gtagcgatct
     8941 tcccgactta actccgcgtc atcgctcagg gcgattaata attcgttcat atgggcaatg
     9001 acgctctctt caatcgcggc atccacgcgc gcgatgactt catccagatg cgacatactt
     9061 tctccttagc cacttgtgaa atttatcgat tcatccaaat gatataagtc tgcgtggtgc
     9121 cgtcaagcca gtgagtaaga tttaaaacag tgaaaacaac agcgccaccg ggaaactcat
     9181 cggccatgtt gcccccacca taaaggcgct taagaaacgg atgcgtttct tatcgtgaga
     9241 aagaaaccag gtgataagaa atgcgattgc tgccataacc gcataaaaaa caagcatgtg
     9301 ttgatagaga gacatgtacg ggcgtccagc cagatgaata aaacgcgcgc aaatatgcgt
     9361 cattttttga cgtgcatcaa gttttttcac cagtttgaga aaaaatcatc aaacagcaac
     9421 ttgcccttat tcatcttcag aatgagtatt attgcggatt ttcttaaccc gtactataca
     9481 cataggggca atgataaaag gtggcaaaaa tgaatgtttc cagtagaact gtagtactga
     9541 taaatgtctt tgctgctgtt ggtttgttta ctcttatctc tatgagattt ggctggttta
     9601 tttgatgtag tgattttgca cgcagcacgt actgtcagag aaatcagtgc tctgacagta
     9661 cgcaagatca tctcatactc ttacgatctg caatattatc cttaatgttt aaccatgacg
     9721 tggcggacga cggtgtaatc ctccagccca taaagtgaca tatccttgcc gtaaccagaa
     9781 agtttctgtc caccgtgcgg catttcactt accagcatga aatgggtatt gacccaggta
     9841 caaccatatt gcagccgtgc gctgacgcga tgcgccctgc ccacatcttt cgtccatacc
     9901 gaagatgcaa gtccgtactg gctgtcattc gcccagttca ccacctgttc ttcgttgtcg
     9961 aagagcgtaa cactcactac tggaccaaat acctcttttt gcacgatggc atcgtcctgt
    10021 aatgcgccag ccagcagcgt cggcgcatag taatagccat tacccttgcg cttttcaccg
    10081 ccagtgatca ctttgatgtg ccctgtcgct ttcgcctctt ctactgcctt gctgacgcgt
    10141 tcgagatgcg cca
//
LOCUS       DAMZET010000151        10145 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.151, whole genome shotgun sequence.
ACCESSION   DAMZET010000151 DAMZET010000000
VERSION     DAMZET010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.151"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..151)
                     /locus_tag="POB34_003766"
     CDS             complement(<1..151)
                     /locus_tag="POB34_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HDI5920636.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTC"
     gene            383..1264
                     /gene="yddG"
                     /locus_tag="POB34_003767"
                     /pseudo
     CDS             383..1264
                     /gene="yddG"
                     /locus_tag="POB34_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
     gene            1523..1813
                     /locus_tag="POB34_003768"
     CDS             1523..1813
                     /locus_tag="POB34_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDI5920637.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANCSRGKNL"
     gene            1837..3167
                     /locus_tag="POB34_003769"
                     /pseudo
     CDS             1837..3167
                     /locus_tag="POB34_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
     gene            3491..4879
                     /gene="narU"
                     /locus_tag="POB34_003770"
     CDS             3491..4879
                     /gene="narU"
                     /locus_tag="POB34_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HDI5920638.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            4961..8701
                     /gene="narZ"
                     /locus_tag="POB34_003771"
     CDS             4961..8701
                     /gene="narZ"
                     /locus_tag="POB34_003771"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HDI5920639.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSSSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            8698..>10145
                     /gene="narH"
                     /locus_tag="POB34_003772"
     CDS             8698..>10145
                     /gene="narH"
                     /locus_tag="POB34_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDI5920640.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDG"
BASE COUNT         2613 a         2514 c         2425 g         2593 t
ORIGIN      
        1 tgcaggtgtt acggatctct ttagcgcgta ataatttgta gtttcgcgcc tgagccagtg
       61 cttgcttcgg ggcaaagccc aatgccgcta ctgttgttcc agccataccg cccgcgcaga
      121 ttttaaaaaa ttgtctgcga ctgacgtcca ttgctttcct cttttttcag gggtattact
      181 gcgcgaggaa actaacagta aaattctgaa tctttttgcg cgaaatcaaa aagaacacgt
      241 gtttgccaca aaataatccg cctgcgcaaa ttgacctacc tcaatagcgg tagaaaaacg
      301 caccactgcc tgacaggcca gttaaaaaaa tgctataaaa ttcagcttaa tttttaacgg
      361 caagagagac aaaacagcga gcatgacacg acaaaaagca acgctcatag ggctaatagc
      421 gatcgtcctg tggagcacga tggtaggatt gattcgcggt gtcagtgagg ggctcggccc
      481 ggtcggcggc gcagctgcta tctattcatt aagcgggctg ctgttaatct tcacggttgg
      541 atttccgcgt attcggcaaa tcccgaaagg ctatttactc gccgggagtc tgttattcgt
      601 cagctatgaa atctgtctgg cgctttcctt agggtatgcg gcgacccgtc atcaggcgat
      661 tgaagtgggt atggtgaact atctgtggcc cagcctgaca attctctttg ccattctgtt
      721 taatggtcag aaaaccaact ggttgattgt acctggatta ttattagccc tcgtcggcgt
      781 ctgctgggtg ttaggcggtg acaatgggtt acattatgat gaaatcatca ataatatcac
      841 caccagccca ttgagttatt tcctggcgtt cattggtgcg tttatctggg cagcctattg
      901 cacagtaacg aataaatacg cacgcggatt taacggaatt accgtttttg tcctgctaac
      961 gggagcatgt ctgtggattt actattttct cacgccacaa ccagaaatgg tatttagcac
     1021 gcccgtcatg attaaactca tctctgcggc atttacctta ggatttgctt atgctgcatg
     1081 aaatgtcggt atattgcatg gcaatgtcac cataatggcg gtaggctcgt attttacgcc
     1141 tgtactttcc tcagcgcttg cagccgtgct gctcagcgcc ccgctgtcgt tctcgttctg
     1201 gcaaggcgcg ctaatggtct gcggcggttc cctgctctgc tggctggcga catgtcgtgg
     1261 ttaaaataat tgccgggtct acggcccggc agttaatcag ataaattccg acaatattaa
     1321 aaaataaatt tcaaatacca gtaattcacc ttatatctca tttcgacttc gatcacatat
     1381 cgacaaataa tattatttca tctatttttc tcattgtttt taatcaccat tctattttaa
     1441 aaacagtatg tcacttataa atgacgccgt ttaaaattcg tttagaaaat acgtttaacg
     1501 ataattaaaa ggattctgtt aaatgaaatt aaaaatagtt gcggtggttg taactggttt
     1561 gttagctgcg aacgtagcac acgctgccga agtctataac aaggatggta ataaactcga
     1621 cctttatggc aaggttaccg ctctacgtta ttttactgat gataagcgtg acgatggtga
     1681 taaaacttat gcccgtctcg gctttaaagg agaaacgcaa atcaatgatc aaatgattgg
     1741 ttttggtcac tgggaatatg attttaaagg ctataacgat gaagccaact gctcgcgcgg
     1801 caagaatctc tgattgagtt aagtgcccat cacaatatga tcactgacct tatattacac
     1861 aatcatccca ggatgaaaac aatcacttta aacgacaacc atattgcaca tttaaacgcc
     1921 aaaaacacta caaaactgga atatttaaac ttaagcaata acaatttact gccaaccaat
     1981 gacattgatc aactaatatc atcaaaatat ctttggcatg tattagttaa cggcatcaac
     2041 aatgatccac ttgcacaaat gcagtactgg actgcagtaa gaaatataat tgatgacact
     2101 aatgaagtga ccattgattt atcaggactt aatttaacca ctcaaccacc agggctgcaa
     2161 aacttcacct ctatcaatct tgataataac caactcacac attttgatgc aaccaactac
     2221 gatagactcg taaagctaag tctgaatatt aatgctcttg agtcaataaa ttttcctcaa
     2281 ggcagaaatg taagtattac acatatatct atgaataata atgctctcag aaatattgat
     2341 atagataggc tttcatcagt tacttatttt agtgcggcac ataatcaact agagtttgtg
     2401 caattagaat cttgcgaatg gctgcaatac ctgaatctca gccataatca attaactgat
     2461 attgttgcag gtaataaaaa tgaactctta ctgctggatc tatcccataa taaactaaca
     2521 agtttacaca atgacttatt tcccaacttg aatacgttac ttattaacaa caatttgctt
     2581 tctgaaatta aaatattcta tagcaacttc tgcaatgttc agacattaaa cgctgctaac
     2641 aaccagttga aatatataaa tcttgatttc ctgacttatc ttccatctat caaaagttta
     2701 agactggaca ataataaaat aacccacatt gatactaata atacatccga tattggaact
     2761 ttattcccca taataaaaca gagcaaaact taaatttttt aaatgtttct gggaagaaca
     2821 attgccctac tatgcagctc atgttattta atttgttttc cccagcactt aagcttaata
     2881 ctggcctggc aattctttcg cctggtgcat ttgaagttca ctctgacgga atagatgtgg
     2941 ataacgaatt gtttcactat cctattaata aagcatatac cccatataat atacacactt
     3001 acaagacaga ggaagttgta aaccagagga atataaaagt taaaaacatg accttagatg
     3061 aaataaacaa tacttactgt aataacgatt attacaatca ggcaataaga gaggaaccga
     3121 tagaccttct ggacagatcg ttttcctcca gttcatggcc tttttagtga ataaatcatc
     3181 actaattcta taagtgtctg gtcttgttga taatatagtt aatatgtaac cctttagatt
     3241 cacgatctga agggttgaaa catgacatac gttcaaagat taacccccgt ttgaaggtta
     3301 gcttgttagt tacatttagt aacacatatc tggctactta atccccattc tgcccccgcg
     3361 gttacgcttt cttatctatt ctttcaatca gcacttcacc caattcatcc ttaattctaa
     3421 taactggcta ttgcgcctta cctcctgttt tgtctttttt cattaccaat gtgtgcatgt
     3481 gaggaacaat atggcactgc aaaatgagaa aaatagtcgt tatcttttac gcgactggaa
     3541 accagaaaat cctgccttct gggaaaataa aggaaaacat attgctcgaa gaaacctctg
     3601 gatatcagtc agttgtctac ttcttgcctt ctgtgtctgg atgctattta gcgcagttac
     3661 cgttaatctc aataaaatcg gttttaattt tactaccgat caactctttt tattaaccgc
     3721 attaccctcc gtttctggcg cattattgcg tgttccctac tcctttatgg tgcctatatt
     3781 cggtggacgc cgatggacgg tttttagtac tgcaatcctg attattcctt gcgtctggct
     3841 cggaattgcc gtgcaaaatc cgaatactcc ttttgggata tttatcgtta tcgctttgct
     3901 atgcggtttt gcaggtgcaa actttgcttc gagcatgggc aatatcagtt tcttctttcc
     3961 aaaagccaaa caagggagcg ctcttgggat taatggcgga ttaggaaact taggtgtaag
     4021 tgtaatgcag ctggttgcac cgctggtcat ttttgtacct gtatttgcct ttctcggcgt
     4081 caatggcgta ccgcaggccg acggttcggt gatgtcgctg gcgaatgccg catggatttg
     4141 ggtaccgcta ctggcgattg ccacgatcgc tgcctggtca gggatgaatg atatcgccag
     4201 ttcacgcgcc tcaattgccg accagctccc tgtcttacaa cgcctgcatc tctggctgct
     4261 gagcctgctt taccttgcca ccttcggttc gtttatcggt ttttctgcgg gttttgccat
     4321 gctggcaaaa acccagttcc cggatgtgaa tattctgcgc ctggcgttct ttggcccatt
     4381 tatcggtgcc atcgcgcggt cggttggtgg tgctatttcc gataagttcg gcggcgtgcg
     4441 ggtgacgttg atcaacttta tttttatggc gattttcagt gccctgctgt tccttacctt
     4501 accgggcaca ggctccggta atttcatcgc cttttacgcc gtatttatgg ggctgtttct
     4561 gactgcgggt ctgggaagtg gttctacttt ccagatgatc gccgtcatct ttcgccagat
     4621 aaccatttat cgggtaaaga tgaaaggcgg tagtgatgag caagctcaga aagaagccgt
     4681 caccgaaacg gcggcggctc tgggctttat ctcagccatt ggcgcagtgg gcggcttttt
     4741 tattccgcag gcgtttggca tgtcgctcaa tatgaccggc tctccggtcg gcgcgatgaa
     4801 agtgttttta atcttctaca tcgtttgtgt gctgctgacc tggctggttt atggtcggcg
     4861 gaagttcagc caaaaataag agatcgatgc ctgatgtaca accgtgtcgc atccgacaag
     4921 gcatcagatc ccaattcatt cctggagcag gagtcatgtc atgagtaaac ttttggatcg
     4981 ctttcgctac ttcaaacaaa agggcgaaac ctttgccgat ggtcacggac aggtgatgca
     5041 tagcaaccgc gactgggagg acagctatcg ccagcgttgg cagttcgaca aaatcgtgcg
     5101 ttccacccac ggtgttaact gtacaggctc ctgtagctgg aaaatctacg ttaaaaatgg
     5161 tctggtgacc tgggaaatcc aacagaccga ctacccgcgc actcgccctg acctgcccaa
     5221 tcatgaacct cgcggctgcc cgcgtggcgc aagttactcc tggtatcttt acagcgctaa
     5281 ccgcctgaaa tacccgctca ttcgtaaacg actgattgaa ctgtggcgcg aagccctcaa
     5341 gcaacacagc gatccggtac tggcgtgggc atcgattatg aacgatccgc aaaagtgcct
     5401 gagctacaaa caagtgcgtg ggcgcggcgg gtttatccgc tccaactggc aggaactaaa
     5461 ccagctgatt gccgccgcta acgtctggac catcaaaacc tacggcccgg atcgcgttgc
     5521 cggtttctcg ccgatcccgg cgatgtcgat ggtttcttac gccgccggaa cgcgttatct
     5581 gtcgctgctt ggcggcacct gtttaagttt ctacgactgg tattgcgacc tgccgcccgc
     5641 ctcgccgatg acctggggcg agcaaaccga cgtaccggaa tctgccgact ggtataactc
     5701 cagctacatc atcgcctggg ggtctaacgt accgcagaca cgtacgccgg acgcccactt
     5761 ctttaccgaa gtacgctaca aaggcactaa aaccatcgcc attacccctg actactctga
     5821 agtggccaaa ttgtgcgacc agtggctggc accgaaacaa ggcactgata gcgccctggc
     5881 gatggcaatg ggccatgtga ttttaaaaga gtttcatctc gataatccca gcgactactt
     5941 tatcaactac tgccgccgct acagcgacat gccgatgctg gtaatgctgg agcctcgcga
     6001 cgatggtagc tacgttcccg ggcgcatgat ccgcgcatct gacctggtgg atggactggg
     6061 cgaaagcaac aatccgcagt ggaaaaccgt agcagttaat accgcaggtg aattggtagt
     6121 gccgaacggt tcgattggtt tccgctgggg agaaaaaggc aaatggaatc tggaatccat
     6181 tgccgccggt acggaaaccg aattgtcgtt aaccctgctc ggtcaacatg acgctgttgc
     6241 aggcgtggcc ttcccctact ttggcggcat tgaaaatccg cattttcgca gcgtaaaaca
     6301 caatccggtg ctggtgcgcc aattgcccgt taaaaacctg acgttagtcg atggcaacac
     6361 ctgtccggtg gtcagcgttt atgatttggt actggcgaat tacggcctcg atcgcgggct
     6421 ggaagatgaa aacagtgcga aagattacgc tgaaatcaaa ccgtacaccc cagcctgggg
     6481 tgagcaaatt accggcgtgc cgcgccagta tattgaaacc atcgcccgtg aatttgccga
     6541 taccgcccat aaaacgcatg ggcgctcgat gattatcctc ggcgcaggtg ttaaccactg
     6601 gtatcacatg gacatgaact accgcgggat gatcaatatg ctgatcttct gcggttgtgt
     6661 cgggcaaagc ggtggcggct gggcacacta tgtcggccag gaaaaactgc gcccacaaac
     6721 cggctggttg ccactggcct ttgcgctcga ctggaaccga ccaccgcgcc aaatgaacag
     6781 cacctcgttt ttctacaatc attccagcca atggcgctat gaaaaagtct ctgctcagga
     6841 gttactttca ccgctcgccg atgccagtaa gtacagcggt catctgattg atttcaacgt
     6901 tcgcgccgaa cgtatgggct ggctaccttc tgcgccgcag ctggggcgta acccgctcgg
     6961 gattaaagct gaagccgaca aggccggatt atcccccaca gaatttaccg cccaggcgct
     7021 gaaatcgggc gatttacgta tggcctgcga acaaccagat agcagcagca atcatccgcg
     7081 taatttgttt gtctggcgct ctaacctgct tggctcctcc ggcaaaggcc acgagtatat
     7141 gcagaagtat ctgctgggga ccgaaagcgg gattcagggc gaggaactcg gtgccagcga
     7201 cgggatcaaa ccggaagaag tcgagtggca aactgcagcg attgaaggca agctcgacct
     7261 gctggtgacg ctcgacttcc gcatgtccag tacctgcctg ttctccgata tcgttctgcc
     7321 caccgccacc tggtacgaaa aagacgatat gaacacctcg gatatgcatc cgtttattca
     7381 tccgctttct gcggcggtcg atccggcgtg ggaatcacgc agcgactggg aaatctacaa
     7441 aggtatcgcc aaagcatttt cgcaagtgtg cgtgggtcat cttggcaaag aaaccgacgt
     7501 ggtattacaa cccctactgc atgactctcc ggcagagctc tcacagccgt gtgaagtgct
     7561 cgactggcgc aaaggcgaat gcgatctgat tccaggcaaa accgcgccga atattgtggc
     7621 ggtggagcgc gactaccctg ctacgtatga acgctttacc tcgctcgggc cattgatgga
     7681 caaacttggc aatggcggta aagggatttc gtggaatacg caggatgaaa tcgatttcct
     7741 cggcaaactc aattacacca agcgtgatgg cccggcacag gggcgtccgc tgattgacac
     7801 cgccattgac gcttcagaag tgattctggc actggcaccg gaaaccaacg gtcatgttgc
     7861 agtcaaagcg tggcaggcgc tgggcgagat caccgggcgc gaacataccc atctggcgct
     7921 gcacaaagag gacgagaaga ttcgctttcg cgatattcag gcgcagccgc gtaaaattat
     7981 ctccagcccc acctggtccg gtctggaaag cgatcacgta tcctataacg cgggatacac
     8041 caacgttcat gagttaattc cgtggcgcac gctgtcggga cgccagcagc tctatcagga
     8101 tcatccgtgg atgcgtgctt ttggtgaaag cctggtggct tatcgcccgc ctatcgacac
     8161 ccgtagcgtc agtgagatgc gccagatacc gccaaacggc ttcccggaaa aagcacttaa
     8221 cttcctgacg ccgcaccaga aatggggcat tcactcaacc tacagtgaaa acctgctaat
     8281 gctgacgctc tctcgcggtg gaccgattgt ctggatcagc gaaacagatg cccgagaact
     8341 gaccattgtc gataacgact gggtggaagt attcaacgcc aacggcgcgc tgactgcccg
     8401 cgcggtggtc agccaacgtg taccgccggg catgaccatg atgtatcacg cccaggaacg
     8461 cattatgaat attcctggtt cggaagtaac tggcatgcgc ggcggcattc ataactcggt
     8521 cacccgcgtt tgcccgaaac cgacgcatat gattggcggt tacgcgcagc tggcctgggg
     8581 ctttaactac tacggcaccg tcggctcgaa ccgcgacgaa ttcatcatga tccgcaaaat
     8641 gaaaaacgtt aactggctgg atgatgaagg tcgcgatcag gtacaggagg cgaaaaaatg
     8701 aaaatacgtt cgcaagtcgg catggtgctt aacctcgata aatgtatcgg ctgccatacc
     8761 tgttcggtga cctgtaaaaa cgtctggacc ggacgcgaag gcatggagta cgcatggttt
     8821 aacaacgtcg aaaccaaacc gggcattggt tatcctaaaa actgggaaga tcaggaagag
     8881 tggcaaggcg gctgggtccg cgatgtgaat ggcaagatcc gtccgcgtct gggcagcaag
     8941 atgggcgtaa taaccaaaat cttcgccaac ccggtggtgc cgcagattga tgattactac
     9001 gaacctttca ccttcgacta cgaacatttg catagcgcac cggaaggcaa acatatccct
     9061 actgctcgcc cgcgttcact gattgacggc aaacggatgg acaaagtgat ctggggccca
     9121 aactgggaag aactgctggg cggcgagttt gaaaaacgtg cccgcgaccg caacttcgag
     9181 gccatgcaaa aggagatgta cgggcagttt gaaaacacct tcatgatgta cctgccgcgc
     9241 ctgtgcgaac actgcctcaa tcccagttgc gtggcgacct gcccaagcgg cgctatctac
     9301 aaacgcgaag aagacggcat tgtgctgatt gatcaggata aatgccgtgg ctggcgtttg
     9361 tgcattagcg gttgtccgta caaaaaaatc tacttcaact ggaaaagcgg caagtcagaa
     9421 aaatgtatct tctgttatcc gcgaattgag tcaggccaac ccaccgtgtg ctcagaaacc
     9481 tgcgtgggtc gcatccggta tctgggcgtg ctgctttacg acgccgaccg cattgaggaa
     9541 gcggcgagca ccgagcgcga agttgacctc tatgaacgtc agtgcgaagt gttcctcgat
     9601 ccacacgatc cctcagtgat cgaggaagcc ctgaaacaag gtattccaca aaacgtgatt
     9661 gaagctgccc agcgttcgcc tgtctacaaa atggcgatgg actggaaact ggcgctaccg
     9721 ctgcaccctg aatatcgcac cctgccaatg gtctggtacg ttcctccgct gtcgccgatt
     9781 cagtcctacg cagatgcggg cggtttgccg aaaagcgaag gcgtgctgcc cgccatcgaa
     9841 agcctgcgta ttccggtgca atatctcgcc aatatgttga gtgccggcga taccggtccg
     9901 gtactgcggg cgctgaaacg gatgatggcg atgcgccact atatgcgttc acaaaccgtg
     9961 gaaggcgtta ctgatactcg tgccatcgac gaagtaggcc tgagcgtcgc ccaggtcgaa
    10021 gaaatgtatc gctatctcgc cattgccaac tatgaagatc gttttgttat cccgaccagc
    10081 catcgggaaa tggcgggcga tgccttcgca gaacgcaacg gctgcggttt tacctttggc
    10141 gacgg
//
LOCUS       DAMZET010000152        10119 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.152, whole genome shotgun sequence.
ACCESSION   DAMZET010000152 DAMZET010000000
VERSION     DAMZET010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.152"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..618)
                     /locus_tag="POB34_003773"
     CDS             complement(<1..618)
                     /locus_tag="POB34_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDI5920641.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFE"
     gene            complement(701..1513)
                     /gene="truA"
                     /locus_tag="POB34_003774"
     CDS             complement(701..1513)
                     /gene="truA"
                     /locus_tag="POB34_003774"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDI5920642.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(1513..2526)
                     /locus_tag="POB34_003775"
     CDS             complement(1513..2526)
                     /locus_tag="POB34_003775"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDI5920643.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEACDVFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(2592..3728)
                     /gene="pdxB"
                     /locus_tag="POB34_003776"
     CDS             complement(2592..3728)
                     /gene="pdxB"
                     /locus_tag="POB34_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HDI5920644.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            3827..4822
                     /gene="flk"
                     /locus_tag="POB34_003777"
     CDS             3827..4822
                     /gene="flk"
                     /locus_tag="POB34_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HDI5920645.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(4819..5997)
                     /locus_tag="POB34_003778"
     CDS             complement(4819..5997)
                     /locus_tag="POB34_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HDI5920646.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(6281..7501)
                     /gene="fabB"
                     /locus_tag="POB34_003779"
     CDS             complement(6281..7501)
                     /gene="fabB"
                     /locus_tag="POB34_003779"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HDI5920647.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            7660..9666
                     /gene="mnmC"
                     /locus_tag="POB34_003780"
     CDS             7660..9666
                     /gene="mnmC"
                     /locus_tag="POB34_003780"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HDI5920648.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTIPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(9787..10065)
                     /locus_tag="POB34_003781"
     CDS             complement(9787..10065)
                     /locus_tag="POB34_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HDI5920649.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
BASE COUNT         2473 a         2823 c         2608 g         2215 t
ORIGIN      
        1 ttcgaaaacc ccaggcaata ttgaaacaaa aataatgcca acaatcatca gcttaaggtt
       61 gctctgaatg aaggggagcg tgccgaagaa ataacccgcg taggtaaaaa tcagtaccca
      121 caacagtgcg ccgatcacgt tataagcggc gaaatgacgg tacgacatgt ggcccattcc
      181 cgcaacaaac ggcgcgaacg ttctgacgat cggtacaaaa cgggcgagaa taatcgtttt
      241 gccgccatgt ctctcgtaaa actgatgagt cttatcaaga taactgcgac gaaagatttt
      301 cgaatccggg ttactgaaca gtttgtcacc aaataaccgt ccaatggtat agttcactgc
      361 atcaccgaca atagcggcaa tcaacataag caccaccatc atatggacat tgagatcgtt
      421 agtttccagc gacgccaatg caccagcaac aaacagcagc gaatcaccag gcaggaatgg
      481 cgttaccacc agccccgttt cgcagaacag aatcaaaaac agaatggcat aaacccagac
      541 gccgtactcc gcgaccagct ctgccagatg cacgtcaatg tgcaggataa aatcgatgag
      601 gaaataaatc aggtccatat ttgcctatgc cttgtactcg tcatctttca ggctgtaact
      661 acgttggcta cagcctgaaa tgttccgagc attattctcg ttagtccgcc agaaatagcg
      721 ggcccattgg cggttttgga agatcatacc ggtcagggta atccaccgcg accagataca
      781 gcccttccgc ttttgccgtt gctgccgcca gcgttctgtc ctttgccgcc agcaactctg
      841 ctatccagct ctccggctgg ttgtgggcac cgacttccat caggctgccg acaatattcc
      901 tgaccatatg atgtacaaag gcattcgctt tgatatctac caccacataa ggaccgtgac
      961 gcgtgacatt aatatgcata acgttgcgcc acggggttcg ggactggcac tgcaccgcac
     1021 ggaacgaggt gaaatcattc tcgcccagca agcattgcgc agcccgatgc atccgttcag
     1081 cgtccagcgg ttcgtaaaaa tgggttaccc ctttactcag taccgccggg cgcagccgat
     1141 gattgtagat gatgtagcga taacggcgag ccgtggcgct aaatcgggca tgaaaatcat
     1201 caggtacagc tttaacccaa cgcacggcga tgtcaccagg taaattcgca tttacgccca
     1261 aggtccacgc cgcgtctttg cgctgcgcgg tcgtttcgaa atgcactacc tgcccggtac
     1321 cgtgtacacc agcgtcagta cgcccggcgc agaagacggt aatgggttcg ttcgccacct
     1381 gggagagcgc cttttccagc ttctcctgca cactgcggac ttcattctgc cgttgccagc
     1441 cgtaatactt actgccgtcg tactcaatgc ccagcgcaat tttataaact ggcggttgtt
     1501 gctggtcgga cattagtaca gatactcctg caccagtttc tcggcgattt ttactgccat
     1561 cagcgcgccg ccaaagcgaa cgttatcggc caccgaccag aactggactt gctccggcat
     1621 accgtagtca ttacgcacgc agccaacaga aagatgcggc gtacccgaag catcccctac
     1681 ctgagtcggg aattcgttct cttcagagag cacaatatct tcgccctgag caaacacatc
     1741 acacgcttct tctgccgcca gcggacgcag tgcttcaaag ttgaccatct gggcatgacc
     1801 gtagaatacc ggtgcctgga cgacgcttgc cgaaatcatc agcccttcgt cctgcaggat
     1861 tttgcgtact tcgtcaacga tacgacgttc ttcacgcacg ctaccttcgc tatccggcaa
     1921 caacggcagc atattgaacg ccagttgacg accgaagaaa tcttcttcgt caatcggaat
     1981 gccgttgagc aatttcgcac tctgccccgc taacgcatcg accgcttttt tgccctgggc
     2041 ggaggctgaa atcaggctgg taacactgat acgcgacaaa ccgccctgat cgattaacgg
     2101 tttcagtgcc gccagcagct ggctggtcag actgtctggt acggcgatga cattccggtt
     2161 gcggtaatcg gtcagtacaa acgggtttac ttccggcacc accagcggta cgtcgggttc
     2221 gagagcaaac agtccgctgc tgtcgatcac caggcaacct gagttggtcg cttcttcaac
     2281 ccaggcagcg gtagcttctt tgcctgcgac aaaaaatgcc agctgcgcct gcgtccagtc
     2341 gaattcagcg gcatcctgca cggtgattgt cttaccacca aagcgcagtt gttcgcctgc
     2401 gctttcgtta cgtgccagtg cataaatttc cccaaccggg aactgacgtt cagccagcgt
     2461 ttcaagcagg gcttcgccca cagcgccagt tgcgcccagg acggcaatgt tccagccttc
     2521 agacatggtg gtttactcca gaaatagcaa agctccctgc caatgttaca gcagagagca
     2581 tgaagaagag attaacgtgc cggatgatga acggcgttaa aacccagttt acacagcaat
     2641 gatgccgcac tggcgtcatc acaaattaca tacagagatg accattcacg gcgctcaaga
     2701 tagtttttgc gcagtttatc gaactcaccc ggtatcccgg cgactttacg cagcggtgcg
     2761 tcatcgcggc gcacatcata caccaaatgc accagccttt tcagcgtcgg ttgatcgagc
     2821 gggccatgca gcgtaatgcg accaaactct ggcgcgggca gtaatgtatc cagcgcaacg
     2881 tgctgttcat gcccaataaa cttgctatag gcttcaaaca cttgcgtagt accgcgtgct
     2941 ttaccttcca gggtataacc tgcgatatgc ggcgtgccga tatccacttt tgtcagcaac
     3001 tccacgttaa gttccggttc gccttcccag acatccagca ctacgcttaa cttctggcct
     3061 tcattcaggc aggtcagcaa cgcagtatta tcgacgactg cgccacggca ggcgttaatc
     3121 agaatcgctc ccggcttcag gctacggatc agtttttcat ccgccagatg cagcgttttg
     3181 tacggcccat ctttgaagag tggcgtatgg aaagtcagaa tatccgcatg ctgaactaat
     3241 tcatccagcg agcggaaatc accctcatcc ccacggtcag cgcgaggcgg atcgcaaagc
     3301 aaggttttaa tccctaacgc ttccagtcgc gcttgcaatc gacgtccaac gttaccaacg
     3361 cccacgatcc ccaccgtacg gtcgtgcagt gaaaatccat cgcgttcggc aagcatcagc
     3421 agggaggaga aaacatattc caccaccgca atcgcattac agccaggtgc cgcggaaaaa
     3481 ccaattcccg cctgctttaa ccatgcttca tcgacatggt cggtccccgc tgtggcagta
     3541 ccaacaaatt taatgggttt tcctgccagc aaagattcat tcactttcgt gaccgaacgc
     3601 accatcagcg catccgcgtc tgccagttga gcgacgggga ttggacgccc gggaaccgcg
     3661 gtcacctcac ccaaacggct aaataagtcg cgggcataag gcatattttc atcaacaagg
     3721 attttcacgt ttgtgttacc tgtatgagac gagagttaac cggacaagtg tgccataatc
     3781 tcgcggccag gcatacttgc gaagatttca ggtataagga tacgtaatga tacaacctat
     3841 ttccggccct cctcctgggc agccaccagg tcagggagat aacctgccgt ctggcgcggg
     3901 caatcagcct ttatccagtc agcaacgtac ttcgctggaa agcttaatga cgaaagtgac
     3961 ctcactgacg caacagcaaa gagcagaact gtgggcgggt atcaggcacg atattggtct
     4021 gtcgggagat tcaccgctgc tttcgcgtca cttccctgcc gctgaacaca acctggcgca
     4081 acgtctgctg gccgcgcaaa aaagccattc tgcccgccag cttttagcgc aattagggga
     4141 gtatttacgt ctggggaata atcgtcaggc ggtcacggat tatatccgtc ataactttgg
     4201 tcagacgccg ctgaatcagc tctcaccgga gcaattaaaa accattctca ccctgttgca
     4261 ggaagggaag atggttattc cgcaaccgca gcagcgcgag gcgaccgacc gtcctttatt
     4321 accggcggag cacaatgcgc tcaaacagct ggtgaccaaa cttgcggcgg caacggggga
     4381 acccagcaaa cagatctggc aatcgatgct ggaactttcc ggggtgaaag atggcgagtt
     4441 aattccagcg aaactgttta accatctggt gacctggctg caggcgcgcc agacgttaag
     4501 ccagcaaaat acgccgacgc tggaatcact acagatggcg ctaaaacaac ctttagatgc
     4561 cagtgaattg gcggcgttat cggcatatat ccagcaaaaa tatggccttt ctgcgcaatc
     4621 atcgctttct tctgcccagg ccgaggatat tcttaatcag ctttatcaac ggcgggttaa
     4681 agggattgat ccgcgtgata tgcaaccgct gcttaatcct tttccaccga tgatggacac
     4741 gttgcaaaat atggcaacgc gtcccgcgct gtggatactg ttagtcgcga ttatcctgat
     4801 gctggtctgg ctggttcgtt agccccgacg aaatgacagt atcgtgacaa taattcccag
     4861 caccgcagag atcgccccgg caagaaatac cgaagagtaa ccaaacgtgg tcgccagcat
     4921 tcccgccagc ggcccggaga cgccgagggc gatatcctga aacgcggcgt aaccgcccag
     4981 tgcggtgccg cgaacttgtg aggggacgcg tttaaccacc tccacgccca gcgcaggaaa
     5041 gataagcgaa catccggctc cggttaacgc cgcgcccgct aatgcgaccc acgcccctgg
     5101 ggcttgccag agcagcaaca agcccaccgt ttctacaagc agagagacaa tcgccacttt
     5161 cacgccgcca aaacggtccg gcatccagcc aaacatgacg cgcatcacga caaatgcgcc
     5221 gccaaacgcg gtaagagtaa agcccgccat cgcccatcct ttgctggcaa agtagagcga
     5281 aacgaaagtc ccgataaccg caaaaccaac gccttgtagt gccagcccta accctggttt
     5341 ccagataagc ccgacaacgc tccacagcga tggacgttct cccgccaagg ccggtacttt
     5401 gcgcactgtg ccgttacagg cccatgccag taagggtaat accattgtgg tgatcgccag
     5461 tgcggcaaaa ccgtaatggc tatgaatcaa caggccaagc ggagcaccaa cagcgagggc
     5521 accgtaaatc gccattccat tccatgacat cactttgcca gagtgttttg gccctacgat
     5581 gcctaacccc caggtcagag cgcctgtcag taactggctt tcaccaaagc caagaatcaa
     5641 acgcccgacg accaacaggg caaatttgaa cggtgcggag acaggcaaaa tcgccgccag
     5701 cagcaacgcg ccgccagcca gaccacaagc taacatcccc tgaagcgccg aacgttttgc
     5761 accatattga tcggccagtc gcccggcata gccacgcgtc agcaccgtag ccagaaactg
     5821 aatcccgacg gcaatcccga ccatggtatt gccatagccc agatcatgat gaacaaacag
     5881 cgggataacc ggcaacggca gccctacggt catgtaggtg agaaaaaccg caaaagcgat
     5941 gcggaagagc gaaaaattgg cagaagatcg tgtttcggtt tggcttacag cagtcatgca
     6001 ttactccaga atgcagcgca aggcgaggag tatccccgtc tcatctctct ggtttcaggg
     6061 ttacggtgcg ttggcaggat ttaacgcgta cgtcttttca gaaggaaatc gacaaagcgg
     6121 gaagtttgcc tggaacaggc ggcgattgtc aatgatgtga aaaagggaac catcaggttc
     6181 ccttttgcgt tagtgccgga ggccgcatta gcgcgtaacg tcggatgcga cgctggcgcg
     6241 tcttatccga cctactccga ctactccgac ctactgcgaa ttaatctttc agcttgcgca
     6301 ttaccagcgt ggcgttggtg ccgccgaagc cgaagctgtt agacataacg gtagtcagtt
     6361 cgcgatcggt cgtttcggtc acgatgttca gacccgcagc ctgctcgtcc agctcttcaa
     6421 tgttgatgct cggggcgata aagccgtgtt ccagcatcag cagagagtag atagcttcct
     6481 gtacgccagc agcgcccaga gagtgaccgg tcatggcttt ggttgcagaa atcgccgggc
     6541 tcttatcgcc gaacacttca cggatagctg ccagttcttt cacgtcgcca accggagtcg
     6601 aagtaccgtg agagttcagg taatcgattg gggtatcaac gccatgcatc gccatctgca
     6661 tacaacgtac tgcgccttcg ccagacggag caaccatgtc tgcaccatca gaggttgcgc
     6721 cgtagccaac gatttcagca tagatgtgag caccacgcgc cagcgcgtgt tccagctctt
     6781 caaccactac cataccgccg ccgccagcga taacgaaacc gtcacggtga gcgtcgtaag
     6841 tacgggaggc tttttccggg gtgtcgttgt atttagtaga cagcgcaccc atcgcgtcga
     6901 attcgcaggc catttcccag cacagctctt cgccgccgcc agcaaacacg atgtcctgtt
     6961 tgcccagttg gatctgctct actgcgttac cgatacagtg tgcggaagtc gcacacgcgg
     7021 agctgatgga gtagttaacg ccatgaattt taaacggcgt ggcgaggcag gcagaaacgc
     7081 cggatgccat cgctttggtg accacatacg ggccaaccgc tttcaggccg cgcgggccgc
     7141 gcattgcgtc agcgccgaat acctggaaac gcggggaacc gccgccagaa cctgcaatca
     7201 ggccaacgcg cgggttattc tggtaagctt ccggagaaag gcccgcatct gcgattgcct
     7261 gctccataga aaggaatgca taaatggatg cgtcgctcat aaagcgcaca actttgcggt
     7321 caatgaggcc agtggtatcc agttttacgt tgccccagac gtggctacgc atgccggaat
     7381 ccttcagctc ctgagagaaa gtgatccctg aacgtccttc acgcagagat gccaggactt
     7441 cctgctggtt attaccgatg ctggaaacaa tgcccaggcc agtaatcact gcacgtttca
     7501 ttcaatacct ctgtaagtcg cacatagagt aagtttcgaa tgcacaatag cgtacacttg
     7561 tacgccgaac aagtccgatc agccatttaa tagagaaatt tgcgcagcct tacacacatc
     7621 gctaagatcg agccaccgcc tgtaagacga gtaacttacg tgaaacacta ctccatacaa
     7681 cctgccaacc tcgaatttaa tgctgagggt acacctgttt cccgagattt tgacgatgtc
     7741 tatttttcca acgataacgg gctggaagag acgcgttatg tttttctggg aggcaaccaa
     7801 ttagaggtac gctttcctga gcatccacat cctctgtttg tggtagcaga gagcggcttc
     7861 ggcaccggat taaacttcct gacgctatgg caggcatttg atcagtttcg cgaagcgcat
     7921 ccgcaagcgc aattacaacg cttacatttc attagttttg agaaatttcc cctcacccgt
     7981 gcggatttag ccttagcgca tcaacactgg ccggaactgg ctccgtgggc agaacaactt
     8041 caggcgcagt ggccaatgcc cttgcccggt tgccatcgtt tattgctcga tgaaggccgc
     8101 gtgacgctgg atttatggtt tggcgatatt aacgaactga ccagccaact ggacgattcg
     8161 ctaaatcaaa aagtagatgc ctggtttctg gacggctttg cgccagcgaa aaacccggat
     8221 atgtggacgc aaaatctgtt taacgccatg gcaaggttgg cgcgtccggg cggcacgcta
     8281 gcgacattta cgtctgccgg ttttgtccgc cgcggtttgc aggatgccgg attcacgatg
     8341 caaaaacgta agggctttgg gcgcaaacgg gaaatgcttt gcggggtgat ggaacagaca
     8401 ttaccgctcc cctgctccgc gccgtggttt aaccgcacgg gcagcagcaa acgggaagcg
     8461 gcgattatcg gcggtggtat tgccagcgcg ttgttgtcgc tggcgctatt acggcgcggc
     8521 tggcaggtaa cgctttattg cgcggatgag gcccccgcac tgggtgcttc cggcaatcgc
     8581 cagggggcgc tgtatccgtt attaagcaaa cacgatgagg cgctaaaccg ctttttctct
     8641 aatgcgttta cttttgctcg tcggttttac gaccaattac ccgttaaatt tgatcatgac
     8701 tggtgcggcg tcacgcagtt aggctgggat gagaaaagcc agcataaaat cgcacagatg
     8761 ttgtcaatgg atttacccgc agaactggct gtagccgttg aggcaaatgc ggttgaacaa
     8821 attacgggcg ttgcgacaaa ttgcagcggc attacttatc cgcaaggtgg ttggctgtgc
     8881 ccagcagaac tgacccgtaa tgtgctggaa ctggcgcaac agcagggttt gcagatttat
     8941 tatcaatatc agttacagaa tttatcccgt aaggatgact gttggttgtt gaattttgca
     9001 ggagatcagc aagcaacaca cagcgtagtg gtactggcga acgggcatca aatcagccga
     9061 ttcagccaaa cgtcgactat cccggtgtat tcggttgccg ggcaggtcag ccatattccg
     9121 acaacgccgg aattggcaga gctgaagcag gtgctgtgct atgacggtta tctcacgcca
     9181 caaaatccgg cgaatcaaca tcattgtatt ggtgccagtt atcatcgcgg cagcgaagat
     9241 acggcgtaca gtgaggacga tcagcagcag aatcgccagc ggttgattga ttgtttcccg
     9301 caggcacagt gggcaaaaga ggttgatgtc agtgataaag aggcgcgctg cggtgtgcgt
     9361 tgtgccaccc gcgatcatct gccaatggta ggcaatgttc ccgattatga ggcaacactc
     9421 gtggaatatg cgtcgttggc ggagcagaaa gatgaggcgg taagcgcgcc ggtttttgac
     9481 gatctcttta tgtttgcggc tttaggttct cgcggtttgt gttctgcccc gctgtgtgcc
     9541 gagattctgg cggcgcagat gagcgacgaa ccgattccga tggatgccag tacgctggcg
     9601 gcgttaaacc cgaatcggtt atgggtgcgg aaattgttga agggtaaagc ggttaaggcg
     9661 gggtaatctg ctctcgcagt gtttaccgga tgcggcgtga acgccttatc cgacctacgt
     9721 gtgacgtgta ggcctgataa gacgcgataa gcgtcgcatc aggcatggtg ctccagacgc
     9781 cgcaacttac tgctgtgacg cctgctgaaa caggttttcc cacatatcgg tcaccagcgc
     9841 ctggtcacgc ggcgacaatt caccggcacc aatggctttt tccagactct ggctaacggt
     9901 cgtatgcacc gcctgagcgg agtggtcatc accactttcc agttctgcga tggctaatgt
     9961 caggtggcca cgcaaatacc cactggcaaa caactcatca tcactggcat ggtcaaccat
    10021 accgtcgatt aatgccagaa tgcgtgattc aaactccgcg atcatcttct ttcctcattg
    10081 taaaacgtgg cgctggcttt agttgagcgc ttccggcca
//
LOCUS       DAMZET010000153        10080 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.153, whole genome shotgun sequence.
ACCESSION   DAMZET010000153 DAMZET010000000
VERSION     DAMZET010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.153"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..271
                     /locus_tag="POB34_003782"
     CDS             <1..271
                     /locus_tag="POB34_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI5920650.1"
                     /translation="FVTIPANANASAAAKVVANFLLSPNAQLRKADPAVWGDPSVLDP
                     QKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            244..1779
                     /locus_tag="POB34_003783"
     CDS             244..1779
                     /locus_tag="POB34_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDI5920651.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPWPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPLKDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLSWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGIFAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            1779..2432
                     /locus_tag="POB34_003784"
     CDS             1779..2432
                     /locus_tag="POB34_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI5920652.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            2499..3806
                     /gene="ynjE"
                     /locus_tag="POB34_003785"
     CDS             2499..3806
                     /gene="ynjE"
                     /locus_tag="POB34_003785"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HDI5920653.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(3815..4435)
                     /locus_tag="POB34_003786"
     CDS             complement(3815..4435)
                     /locus_tag="POB34_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDI5920654.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSHQRQ"
     gene            4522..4929
                     /gene="nudG"
                     /locus_tag="POB34_003787"
     CDS             4522..4929
                     /gene="nudG"
                     /locus_tag="POB34_003787"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HDI5920655.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLESFMALRAARPAD"
     gene            complement(4895..5167)
                     /locus_tag="POB34_003788"
     CDS             complement(4895..5167)
                     /locus_tag="POB34_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HDI5920656.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            5403..6746
                     /gene="gdhA"
                     /locus_tag="POB34_003789"
     CDS             5403..6746
                     /gene="gdhA"
                     /locus_tag="POB34_003789"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDI5920657.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIASFVKVADAMLAQGVI"
     gene            complement(6863..7902)
                     /locus_tag="POB34_003790"
                     /pseudo
     CDS             complement(6863..7902)
                     /locus_tag="POB34_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
     gene            complement(8030..9991)
                     /gene="topB"
                     /locus_tag="POB34_003791"
     CDS             complement(8030..9991)
                     /gene="topB"
                     /locus_tag="POB34_003791"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDI5920658.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRALL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
BASE COUNT         2374 a         2664 c         2598 g         2444 t
ORIGIN      
        1 tttcgtcacc attcctgcta acgcaaatgc cagtgctgcg gcgaaggtgg ttgccaactt
       61 cctgctctca cccaatgcgc aactgcgtaa agcggatccc gctgtctggg gcgatccttc
      121 tgttctcgat ccgcaaaaac tgcctgatgg gcagcgcgaa acattgcaat caagaatgcc
      181 gcaagatctg ccgccggtac tggctgaacc gcacgcaggt tgggtaaatg cgctggaaca
      241 agaatggcta caccgttacg gtacgcatta atctttttgc tgtgggcgat ggtggcggtg
      301 atttatgcac cactgatccc ggcagctctc acgctgatat cgcctgcttt gtcgttgaca
      361 cactggcagg cgttatttgc cgatccacag ttaccgcagg cattactggc aacgctggtg
      421 tcgacaacca tcgcggcggt cggggcgttg ttgattgccc tgctggtgat tgtggcgctg
      481 tggccttggc cgaaatggca gcgtatgtgt gcccgtctgc cgtggctgct cgccattccc
      541 catgtggctt ttgccaccag cgcccttctg ctctttgctg acggagggct gctttatgac
      601 tatttcccgt atttcactcc gctaaaggac aaattgggta ttgggctggg cctcaccctt
      661 gcagtgaaag aaagcgcatt tctgctgtgg atcttagcgg cagtattgag cgaaaaacgg
      721 ctgttgcagc aggtcattgt gctggattcg ctgggctaca gccgctggca atgcctgaac
      781 tggctgctct tgccctccgt cgcacctgca ctggcaatgg cgatgttggc gattgttgcc
      841 tggtcgcttt cggtcgtgga tgtggcgatt attcttgggc caggtaatcc accgacgctg
      901 gcagtaatta gctggcagtg gttaactcag ggcgacgcgg atcaacaaac gaaaggcgca
      961 ctcgccagtc tgctgctgat gctgttactc gccgcctacg ttttgctgag ctatctgcta
     1021 tggcgcagct ggcggcgcac tattccccgc gtagatggca ttcgcaagcc tgccacacct
     1081 ttattaccag gcactacgct ggcgagtttt ttacccttaa ccggtgtgct gtgtgtggtt
     1141 ctgctggcga tcctcgcgga tcagtcgacg atcaatagtg aagcactcat caacagcctg
     1201 acaatggggc tggtggcgac attcatcgct ttgctcctgt tactgctgtg gctggaatgg
     1261 gggccacagc gtcgccagtt gtggctctgg ttgcccattc tattgcccgc tctgccactg
     1321 gtggcgggcc agtacacgct ggcgctatgg ctgaacctgg atggaagctg gacggcggtg
     1381 gtctgggggc atctgctgtg ggtaatgccg tggatgctgt ttatcctgca acccgcctgg
     1441 cagcgcattg attcacggtt aattttgatt gcgcaaacac tgagctggtc gcgggccaaa
     1501 atcttctttt acgtgaaatg tccgcttatg ctacgcccta cgctgattgc cttcgcggtg
     1561 ggattttcag tcagtattgc gcagtatatg ccgacgctgt ggctgggcgc ggggcgtttt
     1621 ccgacgctca ccactgaggc ggtggcatta agcagcggcg gcagcaatgg tattttcgcc
     1681 gcccaggctt tatggcaact gctattaccg cttattattt ttgccctgac cgcgttagtc
     1741 gcaaaatggg taggttatgt cagacaagga ctccgctaat gctctgcgtg aaaaatgttt
     1801 cgctacgttt accagaaagc cgcttgctga caaacgttaa ctttacggtg gataaaggtg
     1861 acattgtcac gttaatgggg ccgtctggct gtggaaaatc cactctgttt tcatggatga
     1921 ttggtgcact ggctggacag ttttcttgta caggtgagct atggctcaat gagcaacgga
     1981 ttgacatgct acccaccgca cagcgtcaga ttggcattct tttccaggat gcactgttat
     2041 ttgaccagtt cagcgtcggg caaaatttac tgctggcgct accgtcgacg ctcaagggga
     2101 ctgccaggcg caatgccgtg aaagacgcgc ttgatcgagc gggactggct gaaacttatc
     2161 accaggaccc tgcgacatta tcaggcggtc agcgagcgcg ggtggccctg ctgcgcgccc
     2221 ttctcgctca gccaaaagcg ttactcctgg atgagccatt cagccgtctt gatgtggctc
     2281 tgcgcgataa ttttcgccag tgggtgttca gcgaagttcg cgaactggcg atccccgtcg
     2341 ttcaggtaac gcacgatctc caggatgttc ctgctgatag ttctgttctg gatatggcgc
     2401 agtggtcaga aaattacaac aaactgcgat aacgcaaagt ttttctcaat gcgtcagttc
     2461 agaatggcgc actcaaaact acaatgtcgg gattttcgat gaaacgtgtt tctcaaatga
     2521 ccgcgctggc aatggcttta gggctggctt gcgcttcttc gtgggccgct gaactggcga
     2581 agcctcttac acttgaccag cttcaacaac aaaatggcaa agcgatagat actcgcccca
     2641 gcgcgtttta taacggctgg ccacaaacct taaatggccc ttctggtcat gaacctgccg
     2701 ccttaaacct ctctgccagc tggctcgaca aaatgagcac cgaacagctc aacgcgtgga
     2761 tcaagcaaca taacctgaaa gccgatgccc cggtggcgct gtacggtaat gacaaagatg
     2821 tcgacgccgt caaaacgcga ctgcaaaaag caggttttac gcatatctcc attctgagtg
     2881 acgcgctaag cgaaccttcc cgtctacaaa aactgccgca ctttgagcag ctggtttatc
     2941 cgcaatggct gcacgacctg caacaaggta aagaggttac ggcgaaacct gccggtgact
     3001 ggaaagtcat tgaagcggcc tggggcgctc ctaagcttta ccttatcagc catattcccg
     3061 gtgctgacta catcgatacc aacgaagtgg aaagcgaacc gctgtggaac aaagtttctg
     3121 atgaacaact aaaagcgatg ctggcaaaac acggcattcg ccatgacacc acggtcattc
     3181 tgtacgggcg tgacgtatac gctggagcgc gtgtggcgca aattatgctc tacgcaggcg
     3241 tgaaagatgt gcgtctgctg gatggcggct ggcaaacctg gtccgacgcg ggactgcctg
     3301 ttgagcgagg aacgccaccg aaagtgaaag cggaaccgga tttcggcgtg aagatcccgg
     3361 cacaaccgca gttgatgctc gatatggaac aggcccgtgg actgctgcat cgccaggatg
     3421 catcgctggt gagcattcgt tcgtggccag aatttatcgg tacgaccagc ggttacagct
     3481 atattaaacc aaaaggtgaa atagccggag cacgttgggg acacgctggt agcgactcga
     3541 cgcatatgga agattttcat aatccggatg gcaccatgcg cagcgccgat gatattaccg
     3601 ctatgtggaa agcgtggaat atcaaaccag agcagcaagt ttcattctac tgcggcaccg
     3661 gctggcgcgc gtccgaaacc tttatgtacg cacgcgccat gggttggaag aatgtttccg
     3721 tgtatgacgg cggctggtac gaatggagca gcgatccaaa aaatccggta gcaaccggtg
     3781 aacgcggccc ggacagtagc aaataacatt gcaattactg acgctggtgc gacttcagcg
     3841 tcagataccc actccacacc cgcgtaaagg tcgtcatcca gcacaacgca ccgaagatcc
     3901 acgcaaacca cgcaaaccat gcagggaata aacatcccag cacaaacagt aagatcgttt
     3961 ctgtgccttc ggttaacccg cccagataat aaaacgattt atgcgcataa ccgggattat
     4021 caatctgatg tttcgccgcc agcgcagcaa aagcgagaaa actgctgcct gtgccgataa
     4081 acgcaaacag cagccagccg cctgccaggg cgttttgctg cggtgcggcg aggataaacc
     4141 caaaaggtac cagcgcgtaa aaaagaaaat cgagagaaat atcaagaaag ccgcccgcgt
     4201 cggtaagccc tctgcgccgc gccagcgcac cgtccagacc atcaagcagc ctgttcaaca
     4261 aaatgacgac aagcgccgcc agataccagc ccagcgccag aaaaggcagt gccagcacgc
     4321 cgatggcaaa tcccactaat gttaaaccat ctggcgtaat gcccggttta tcaagaaccc
     4381 gcacgcactg atgcaacaac ggtttaatcc ggggatgaag atggcggtct agcacaggca
     4441 ctccttaaat ataaagcctt tctgattgag caacagtgcg gatattatgg catttttcgc
     4501 ttatctgccc gtgtgtaatt tatgaaaatg attgaagttg ttgccgccat cattgaacgt
     4561 gatggcaaaa ttttactcgc gcaacgcccc gcccagagcg atcaggcggg attatgggag
     4621 tttgccggtg gtaaagtcga gccggatgaa agccagcggc aggcgctggt gcgtgagtta
     4681 cgcgaagaac tgggcatcga agcaactgtg ggtgagtatg ttaccagcca tcagcgagaa
     4741 gtttcgggcc ggattatcca tcttcatgcc tggcacgtac ccgacttcca cgggacgtta
     4801 caggcacatg aacatcaggc gctggtctgg tgctcacctg aagaggcgct gcaatatccg
     4861 ctggcccctg ctgacattcc attattagag tcgtttatgg ctttacgcgc cgccagacca
     4921 gcggattagt gctaagggtt ttgtcatccc gctggcattg cagcagtatt ccctcggctt
     4981 taattaccgc gccttcagaa taatttttat cctgataaac gcagcactga gtacagggct
     5041 gcgctgactg cccgcctgaa ctgaacactt ctggcggtac gtttacctcc acgtccggac
     5101 gataatgcgg gttagccagt gcaattaatg gaaatgctaa tactacggcg aacaatgctc
     5161 gactcacagg gaactcctta acgttattgt ctctgctact gataacggta gccgggtggc
     5221 aaaactttag cgtctgagtt atcgcatttg gttatgagat tactctcgtt attaatttgc
     5281 tttcctgggt catttttttc ttgcttaccg tcacattctt gatggtatag tcgaaaactg
     5341 caaaagcaca tcacataaac aacataagca caatcgtatt aatatataag ggttttatat
     5401 ctatggatca gacatattct ctggagtcat tcctcaacca tgtccaaaag cgcgacccga
     5461 atcaaaccga gttcgcgcaa gccgttcgtg aagtaatgac cacactctgg ccttttcttg
     5521 aacaaaatcc aaaatatcgc cagatgtcat tactggagcg tctggttgaa ccggagcgcg
     5581 tgatccagtt tcgcgtggta tgggttgatg atcgcaacca ggtacaggtc aaccgtgcat
     5641 ggcgtgtgca gttcagctca gccatcggcc cgtacaaagg cggtatgcgc ttccatccgt
     5701 cagttaacct ctccattctc aaattcctcg gcttcgaaca aaccttcaaa aatgccctga
     5761 ctaccctgcc gatgggcggt ggtaaaggcg gtagcgattt tgacccgaaa ggtaaaagcg
     5821 aaggcgaagt gatgcgtttt tgccaggcgc tgatgaccga gctttatcgt catctgggcg
     5881 cggataccga cgttccggca ggtgatatcg gcgttggtgg tcgtgaagtc ggctttatgg
     5941 cggggatgat gaaaaagctc tccaacaata ccgcctgcgt cttcaccggt aagggccttt
     6001 catttggcgg tagtcttatt cgcccggaag ctaccggcta cggtctggtt tatttcacag
     6061 aagcaatgtt aaaacgccac ggtatgggtt ttgaagggat gcgcgtttcc gtttctggct
     6121 ccggcaacgt cgcccagtac gctatcgaaa aagcgatgga atttggtgct cgtgtgatca
     6181 ctgcgtcaga ctccagcggc actgtagttg atgaaagcgg attcacgaaa gagaaactgg
     6241 cacgtcttat cgaaatcaaa gccagccgcg atggtcgagt ggcagattac gccaaagaat
     6301 ttggtctggt ctatctcgaa ggccaacagc cgtggtctgt accggttgat atcgccctgc
     6361 cttgcgccac ccagaacgaa ctggatgttg acgccgcgca tcagcttatc gccaatggcg
     6421 ttaaagccgt cgccgaaggg gcaaatatgc cgaccaccat cgaagcgact gaactgttcc
     6481 agcaggcagg cgtactgttt gcaccgggta aagcggctaa tgctggcggt gtagcaacgt
     6541 caggccttga aatggcgcaa aacgctgcgc gcctgggctg gaaagccgag aaagttgacg
     6601 cacgtttgca tcacatcatg ctggatatcc accatgcctg tgttgagcat ggtggtgaag
     6661 gtgagcaaac caactacgtg cagggcgcga acattgccag ttttgtgaag gttgccgatg
     6721 cgatgctggc gcagggtgtg atttaagttg taaatgcctg atggcgctac gcttatcagg
     6781 cctacaaatg ggcacaattc attgcagtta cgctctaatg taggccgggc aagcgcagcg
     6841 cccccggcaa aatttcaggc gtttatgagt atttaacgga tgatgctccc cagggaacat
     6901 ttcttatggg ccaacggcat ttcttactgt agtgctccca aaactgcttg tcgtaacgat
     6961 aacacgcttc aagttcagca tccgttaact ttctgcgata gcagcagata tgccagtaaa
     7021 gaaatcccat tagactattt ttttgataat cttcttcgct ttcgaacaac tcgtgcgcct
     7081 ttcgagaagc aagcattata taatgccagg ccagttcttc ttcaattgtc ccgttttgaa
     7141 aagctgtgct tgatatcgag atcatccatg ataattccgc cgcccatatt agcttcgccg
     7201 aggatttacc ggagcgatta ttagcgcaat cagagatata gtctgaggga aaaacagcaa
     7261 atttattcaa caaggcgata acctgctctg gggcttcctc catgtttgct ttaaaggtat
     7321 tggctccatg gtcgccagaa agaaaatgct ccattaaggc acaataactt tcgctatctt
     7381 cgatacccca ttgatcctct aaagactcgc gtcttttact tatgatatcg atcgagtcaa
     7441 agggaagcac atgatattgg aagatatctt tgccaggttc aggctttcgc ggccagaact
     7501 ccagcgtttc agaccattgc ttatgataga accgataagg tgcgatcaat tgtagcgcct
     7561 gtaacttctc gatactgagc ggctcaatac ctttagcctg ataataatgc agttgttctt
     7621 tttttgcttt aaaaccggcc cgaacaataa gccccatcat aattaataga taaagaaaag
     7681 agcatcccgc agtaatcagg cctctttcat tcaaaccgtt ggatgttatc gctgcgaata
     7741 caaacattac agcgacaaca catgttaaat acccccccac ttacaaagca gcatggcctt
     7801 attttcttta atcatccgtt caaaattact attaaatatt tcccagccat taaaagaata
     7861 cttctcgctc ccaggatggt tttgtaataa aacttttttc atcacattcc ctgttattac
     7921 atactttaga ggcgataatt ttatccatgc aaaaaaaata tccaacgaat tttcttgatc
     7981 cgctggatat ttcagaatat gactcgaata gcacgaaaga ttcgctcgct tacgctatcg
     8041 ccccgcttcc ggcttcatct gctggcggac tttttttcgc actacgttta cgcggtgcag
     8101 cctttttctt atcagcactg ccaccactgc ccggagccac aatgccgcga aactgccgca
     8161 ccggcgtacg tttggcttga tcaataagtt gatatagcgt ccccaccagc ggctgcataa
     8221 agtcctgata gcgacactgc ttttcgctga tttgcgtcag caccgattcc cagtgcgcgg
     8281 tcatgtccgg tcgcgtcgcc atttctggca gcgaatggaa taacgctttt ccggcgtcgg
     8341 tagagtggat atagcgccct tttttggtca gaaaaccacg cttgaacagc agttcaataa
     8401 tcccggcacg ggttgcttct gtccccagac catcggtcgc acgaaggatc tttttcagat
     8461 ctttgtcctg cacaaagcgc gcaatcccgg tcatcgccga aagcagtgtt gcatcggtaa
     8521 aatggcgcgg cggctgggtt tgccgctcta ccacttcacc tttttcacac agcaactcat
     8581 cgcctttcgc caccacaggc aatggcgtgc cgtcgttttc ttcatcgcgc tctttgctgc
     8641 ctaacagcgc gcgccaacct gcttcagcaa gaaaacgcgc tttagcgaca aatttgcctt
     8701 tggcaatgtc cagttcgata acacacttgc ggaacaccgc atccgggcag aactgcatca
     8761 gatactgacg ggcaatcagg ttatagacct tcgcttcgtt ctccgtcagg ttgatcgcag
     8821 aactccgtgc ggtcggaatg atggcgtggt gcgcatcgac ctttttgtca tcccaacagc
     8881 ggttgcgtat atctggatct accactggct gcggcaacag atctggtgca tgaacgctga
     8941 tggcattcat caccgcgtgg cgtccggcaa aatgttcttc tggcaaatag cgacaatcgg
     9001 aacgcggata agtgattaac ttgtgtgttt cgtacagctt ctggcagata tcaagcacgt
     9061 tctgcgcgct cagaccaaag cgttttgccg cttcaatctg caacgccgaa agcgaaaacg
     9121 gcagcggcgc ggattctgat tcccgtttat cgttatagct ggtgacaata gccggttgac
     9181 cactaatgcg gttaaccaca tgctccgcca gtggacgatg taacaagcgc ccttcttcat
     9241 cctggtacgg ttcacacgct tcgctcggtt gccagatagc ggtaaaccgc tcatcggcag
     9301 gtgtcacgat atgcgctttg acttcaaaga agtctttcgc cacgaagttt tcaatctctt
     9361 catcgcggcg caccaccagc ccgagcacgg gtgtctgcac gcgtcccacg gaaagtacgc
     9421 cctgataacc ggcattgcga ccgagaatgg tatacgcacg ggtcatattg atgccgtaca
     9481 gccagtcggc acgcgctcgc gccagcgcag aaacgcacaa tggcacaaac tcactgttgg
     9541 aacgaagacg gtcgatcgcc cgctcaaccg cctgcgggtt caggtcgttt atcaagcaac
     9601 gctgcacctg ctggcgcttt tccggtgcca gttgcagata gtccagcact tcatccacca
     9661 gcaattgccc ttcacgatcc gggtccccgg cgtgaacgat ttcgctggct tcatgcagga
     9721 agcgtttgat gacgttaagt tgtttggtca cggagggtcg gggctgtaat tgccactttt
     9781 ccgggacaat cggcaaatcc gcaagattcc agcgcgcata gcggctgtcg taggcgtctg
     9841 gctgcgcctg ctcaagcagg tgaccgatac accaggtcac cacctgacca ttaccgcact
     9901 cgataaagcc atcgccttta cggtgcggtt tgggcaggac atcagcaatg gcgcgcgcca
     9961 gactcggttt ttcggcaata aacaaccgca ttgaattaac ggatctcaac catggcacga
    10021 ccgccgcgcg ctggcaccag ttcgccaatc gccgtcagtt caatgccaaa ctcggcggct
//
LOCUS       DAMZET010000154        10051 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.154, whole genome shotgun sequence.
ACCESSION   DAMZET010000154 DAMZET010000000
VERSION     DAMZET010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.154"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..264
                     /locus_tag="POB34_003792"
     CDS             <1..264
                     /locus_tag="POB34_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI5920659.1"
                     /translation="LWRGGQVLAVPPAYTSQRCAYCGHTAKENRLSQSKFRCQVCGYT
                     ANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
     gene            complement(304..1518)
                     /gene="ydcO"
                     /locus_tag="POB34_003793"
     CDS             complement(304..1518)
                     /gene="ydcO"
                     /locus_tag="POB34_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HDI5920660.1"
                     /translation="MLRCLYAITYEVTMRLFSIPPPTLLAGFLAVLIGYASSGAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTISGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            1571..2107
                     /gene="sutR"
                     /locus_tag="POB34_003794"
     CDS             1571..2107
                     /gene="sutR"
                     /locus_tag="POB34_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HDI5920661.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            2180..4141
                     /gene="rlhA"
                     /locus_tag="POB34_003795"
     CDS             2180..4141
                     /gene="rlhA"
                     /locus_tag="POB34_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HDI5920662.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(4233..4463)
                     /locus_tag="POB34_003796"
     CDS             complement(4233..4463)
                     /locus_tag="POB34_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HDI5920663.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4685..4839
                     /locus_tag="POB34_003797"
                     /pseudo
     CDS             4685..4839
                     /locus_tag="POB34_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            4885..5301
                     /gene="hicB"
                     /locus_tag="POB34_003798"
     CDS             4885..5301
                     /gene="hicB"
                     /locus_tag="POB34_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HDI5920664.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5380..6786
                     /locus_tag="POB34_003799"
     CDS             5380..6786
                     /locus_tag="POB34_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDI5920665.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            7031..8176
                     /locus_tag="POB34_003800"
     CDS             7031..8176
                     /locus_tag="POB34_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI5920666.1"
                     /translation="MSKTFARSSLCALTMTIMNAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            8194..9207
                     /locus_tag="POB34_003801"
     CDS             8194..9207
                     /locus_tag="POB34_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5920667.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            9208..>10051
                     /locus_tag="POB34_003802"
     CDS             9208..>10051
                     /locus_tag="POB34_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5920668.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIG"
BASE COUNT         2480 a         2513 c         2621 g         2437 t
ORIGIN      
        1 ctctggcgtg gcggtcaggt gcttgctgtt ccgccagcgt atacaagcca gcgttgcgcg
       61 tactgtggtc atacagcgaa agagaaccgc ctgtcacaaa gtaaattcag atgccaggta
      121 tgtggatata cagcgaacgc cgatgtaaat ggcgctcgta acattttagc ggcggggcac
      181 gccgttcttg cctgtggaga gatggtgcag tcaggccgct cgttgaagca ggaacccacc
      241 gaaatgattc aggcgacagc ctgaacgtag cagggatcca cgtccttcag ggcgtggagg
      301 atgtcaatat cggtttctgt cagcgattaa attcaacacc acgtaacaaa cgcctccggc
      361 aattaatccc cagaacgcag aaccaatccc gaccagcgtc aatccacttg ccgttaccag
      421 aaatgccacc accgccgcgt ctcgctcacg ctcattatgc agcgcctgat acaaactgcc
      481 gctaatggta cttaacagcg ccagacctgc cagcatctgg atccaactta cgggcagggc
      541 agccatcatc ccggtaatag cactaccaaa cagacctgcg atcaaataaa aaatgcctgc
      601 aacggcagct gccagccaac gttgatcttt atccggatgc gcttccgggc tttggcaaat
      661 agccgcggtg attgccgcaa taccgacgga ataaacgccg aaaggggaaa aaaccagtgc
      721 cagcaatcca gtaaatacaa ttaatggcga aacaggagcc gaatacccgg ctgctttcat
      781 tgctgcgata cccggtgcgt tttgcgatgc catcgtcacc agaaaaaggg ggagtgcaac
      841 gctcaggctg tgagcaaacg aaaaatcagg ggtaatataa gtggggagaa cgggtttaaa
      901 gacaacatca gttgtgacaa cgtcaccttg cgcgataacg atcacgatcc caataatcat
      961 cgcggcaatt accgcatagc gcggcgcaac ggccttggtt gccagccata ccagcaacat
     1021 acttccacac aacgtaaatt gaccgtccag actggcaaac gcctgtaaac caaagcgtaa
     1081 taaaatcccg gcaagcattg ccgccgcaag cgagtgcgga ataatgcgca tcagacgagc
     1141 aaagagtccc gttatgccgc agaggactat taacgcgttg gtgacaataa aaacgccgat
     1201 ggcttcgtta agtgttagtc cctgcaatcc ggtgaccaac aaagccgcgc caggcgttga
     1261 ccatgcggtg agaacaggta cgcgatacca taatgtcaga gtcagcgtac tgacgcccat
     1321 tgccagcccc agcgccgtca tccagccaga gatttgtgca gtggtggctc cggcgacaat
     1381 cgctgcttgc cagattattg cccctgaact ggcgtagcca attaataccg ccagaaaccc
     1441 cgccagtagc gtgggtggag gaatagaaaa cagacgcata gtcacctcgt acgttatagc
     1501 gtacaggcac cgtagcattt gtccgttata acgcacaagt gataaacttc cgttttgccg
     1561 gaggagtcgc atggaaaatc tcgctcgctt tttatccacc acacttaaac aactacgcca
     1621 gcagcgcggc tggagtcttt cgcgactggc agaagcgacg ggcgtttcta aagcaatgtt
     1681 agggcagatt gagcgtaatg agtccagccc gacggtcgcg acattatgga aaattgccac
     1741 cgggttgaat gtgccatttt cgacatttat ttctccgcca cagtccgcta cgccttcagt
     1801 ttatgatcca caacagcagg cgatggtgat cacttcgctg tttccttatg atccacaact
     1861 ctgctttgaa cacttctcga ttcagatggc acccggtgca ataagcgaat cgacgcccca
     1921 tgagaagggg gtaatcgaac atgtcgtcgt cattgatgga caacttgatc tgtgcgttga
     1981 tggggaatgg cagaccctta attgtgggga aggcgttcga tttgctgcag acgtcacgca
     2041 tatctatcgt aatggtgggg agcaaaccgt acattttcat tccctcatcc attacccgcg
     2101 cagttaagcg ggaaaactat ttcgcaacgt cgcgcttgtg gctaaaatag ccgccatttt
     2161 tcagctactg gataagaatg tgaccgtatc ttctcatcga cttgaactgt taagcccggc
     2221 acgcgatgcc gccattgccc gcgaagctat tttgcacggt gccgatgctg tttatatcgg
     2281 cggccctggt tttggtgccc gtcataatgc cagtaatagc ctgaaagata ttgccgagct
     2341 ggtgccgttt gcccatcgtt atggtgcaaa aattttcgtc acgcttaaca ccattttgca
     2401 tgatgatgag ctggaacccg cgcaacggct gattactgac ctctaccaga ccggtgtcga
     2461 tgcgctgatt gttcaggata tggggattct ggaacttgat attccgccga ttgaactgca
     2521 cgccagtacg cagtgcgaca ttcgtacagt tgaaaaagcg aagttcctct ctgatgttgg
     2581 cttcacgcag attgtgctgg cgcgagagct gaatcttgat cagatccgcg cgattcacca
     2641 ggctacggac gcgaccattg aattctttat tcatggggca ctgtgcgtgg cctattcggg
     2701 tcagtgctac atttctcatg cgcaaacagg gcgtagcgcc aaccgtggcg attgctcgca
     2761 ggcgtgccgt ttgccataca cattgaaaga cgatcagggg cgggtggttt cctatgaaaa
     2821 acatctgctg tcgatgaaag ataacgatca gactgccaac ctcggcgcgc tgattgatgc
     2881 tggtgtacgc tccttcaaga ttgaagggcg ttacaaagat atgagctacg tgaagaatat
     2941 caccgcccat tatcgccaga tgcttgatgc cattattgaa gaacgtggcg atctggcgcg
     3001 cgcttcatca ggtcgtactg aacatttctt tgttccatcg acggaaaaga ctttccaccg
     3061 tggtagcaca gattattttg tgaatgcccg taaaggcgat attggcgcgt tcgattcgcc
     3121 gaaatttatc ggcctgccgg taggcgaagt attgaaagtg gcgaaagatc atctcgatgt
     3181 tgccgttacc gagccactgg caaatggcga tggcctgaac gtgttgatta aacgtgaagt
     3241 cgtcggtttt cgtgccaata cggtcgagaa aaccggagaa aatcagtacc gcgtctggcc
     3301 caatgaaatg ccagcagatt tgcacaaaat tcgtccacat cacccactaa accgtaatct
     3361 tgatcataac tggcagcagg cactgacaaa aacctccagc gaacgtcggg tggcggtaga
     3421 cattgaactg ggcggctggc aggaacaact gattctgacc ctcaccagtg aagagggtgt
     3481 cagcatcacg catacgctgg acgggcagtt cgacgaagcc aataacgccg aaaaagcaat
     3541 gaacaatctg aaggatggtc tggcaaaact ggggcaaacc ctctattacg cccgcgatgt
     3601 gcaaattaat ttgccggggg cgctgtttgt accaaacagt ctgttaaacc agttccgccg
     3661 tgaagctgct gacatgctgg atgctgcgcg tcttgccagt taccagcgcg gcagccgtaa
     3721 accggttgct gatcctgcgc cggtttatcc gcaaacgcat ctgagtttcc tcgcgaacgt
     3781 atacaaccag aaagcgcgtg aattttatca tcgctatggt gtgcagctga ttgacgcggc
     3841 gtatgaagca catgaagaga agggcgaagt cccggtgatg atcaccaagc attgtctgcg
     3901 ctttgccttt aatctgtgcc cgaaacaggc gaaaggcaat atcaaaagct ggaaggcgac
     3961 gccaatgcaa ctggttaacg gcgatgaagt attaacgcta aagtttgatt gccgcccatg
     4021 cgagatgcac gtcattggca aaatcaaaaa tcacatactg aaaatgccgt taccgggaag
     4081 cgtagtggca tccgtaagtc cggatgagct gctgaaaaca ttgcctaagc gaaaagggta
     4141 aaacgccagt tttctggtta ctcacaactc attgaatctg catgatattg cctgccgggt
     4201 aaggcgtcac gccgcatccg gcaacaaatg actcaacgtt gtcccgcttc tggtttgcgc
     4261 gatttttgcc agtaaaaatg ctcgcgcaaa ccttccgccg actcttccgc cacagcacgc
     4321 aattcatcgc tgtccgcttc atgacgcagc tgatgatcca cattctttac ccacacaaat
     4381 tcatgtcctt tgtgccctgc catgagttgt cctgagaaca gcgcacacgt taataagaca
     4441 accgataacg ccttcgtaaa catcctgcca cctttttctt accttttgcc gctatgatgc
     4501 cgatcgtttc ttgaggttat tattcagttt tgcaaattag cgcaaagaaa ttctggaatc
     4561 ttccttcctg attttgcatt gcattctgcc gttgcggcga tttagtgcta ttttcgagca
     4621 aattacacac agaggtaaac actaatgttt actttgttga tatactcagc ggcagggagg
     4681 cgatgtgaaa caaagcgagt tcagacgttg gctcgaatct cagggcgtcg atgtagcgaa
     4741 tggccgcaac catttgaaac tcaggtttca tgggaggcgc agtgtcatgc cgcgtcactc
     4801 ctgcgtaaag caatcctgaa acaactcggt ttgagttaat cgccaattaa aaaggttaat
     4861 gacatgcgag agacagtcga aattatgcgt tatcccgtca ctcttacacc cgcgccggaa
     4921 ggcggttata tggtttcttt tgtggatatc cctgaagcgt tgacgcaggg cgaaactgtc
     4981 gctgaagcga tggaagcggc aaaagatgct ttactgaccg catttgattt ttattttgaa
     5041 gataacgagc ttatcccgtt accttcgcca ttaaatagtc atgatcactt tattgaagta
     5101 cctttgagcg tcgcctctaa agtattgctg ttaaatgctt ttttacagtc agaaatcact
     5161 cagcaagagt tagccaggcg aattggcaaa cctaaacagg agattactcg cctatttaac
     5221 ttgcaccatg cgacaaaaat cgacgccgtc cagcttgcgg caaaggcgct tggcaaagag
     5281 ttatcgctgg tgatggttta attacagtta acgaaaagtt gtcattttta acaactgata
     5341 tagactgccg aatcatctgc acataattac gattcgataa tgaaaaaata ccagcagctt
     5401 gcagaacaat tacgcgagca gattgcgtcg ggtatctggc aacccggcga tcgtttgcct
     5461 tcgttgcgtg accaggtggc gctttcaggc atgagcttta tgactgtcag ccatgcctat
     5521 cagttgctcg aaagtcaggg atatattatc gcacgaccgc agtcgggtta ttacgttgcg
     5581 ccacaggcaa taaaaatgcc gaaagcgcca gtcattccag tcactcgaga tgaagcagtc
     5641 gatatcaaca cttatatttt tgatatgttg caggccagtc gcgatccgtc ggtcgttccg
     5701 tttgcctcgg cctttcccga cccgcgactt ttccccctcc aacaactaaa ccgctcgctg
     5761 gcgcaggtaa gcaaaaccgc cacagcgatg agcgtgattg aaaacttacc gccaggaaac
     5821 gcagaactgc gtcaggctat tgctcgtcgc tatgccttac agggcatcac catttctcct
     5881 gatgaaattg tcattactgc cggggcgtta gaggcattaa acctcagttt gcaagcggta
     5941 actgaaccgg gcgattgggt gatagtagag aatccttgtt tctacggtgc gttgcaggcg
     6001 ctggagcggc tacggctgaa ggcgttatcg gtggcgacgg atgttaaaga agggatcgat
     6061 cttcaggcgc tggaactggc gttgcaggat tatccggtga aagcgtgctg gctgatgact
     6121 aatagccaga acccactcgg atttacctta acgccgcaaa aaaaagcaca actggtggca
     6181 ttactcaatc agtacaacgt aacgctgatt gaagatgacg tttacagcga actttatttt
     6241 ggacgggaaa aaccgctgcc tgcgaaagcg tgggatcgcc acgatggcgt tttgcattgc
     6301 tcttcgtttt cgaaatgtct ggtgcctggt tttcgtattg gttgggtcgc cgccggaaaa
     6361 catgcacgta aaattcaacg cttgcagttg atgagtacgc tttccaccag ctcaccgatg
     6421 caacttgcgc tggtggatta cctttccacg cgccgatacg acgcccatct tcgtcgcctg
     6481 cgtcgccagc ttgcggaacg taaacaacgt gcctggcagg cactgctgcg ttatctgcct
     6541 gcggaagtta aaattcatca tagtgacagc ggttattttc tctggctgga gctccccgag
     6601 ccgttagatg ccggcgaatt aagcctggcg gcactgacgc atcatatcag tattgcgccg
     6661 ggtaaaatgt tttctaccgg tgaaaactgg tcacgttttt ttcgttttaa taccgcgtgg
     6721 cagtggggag aacgtgaaga acaggcggta aaacaattag gcaaacttat tcaagaacgg
     6781 ctgtaatagc gtttaattta attcctctta gattgggtaa tatgaatttc gaatagcagt
     6841 catatttcct aaccccttgt ctatactcca gaagataacc ttacagacgg cataatgcgc
     6901 ggtagctcac aacctgatta aattttctca ggggcgaagg tgtgcctgca agccgccgtc
     6961 tatggttaaa caaggagata tttttacggc acggcggctg aacaattaat tacgacagga
     7021 gtaagacctt atgagcaaga catttgcccg cagcagcctg tgtgcgctca ccatgacaat
     7081 aatgaacgct cacgccgccg aaccacctac caatttagat aaaccggaag ggcgactgga
     7141 tattatcgcc tggccgggat acatcgaacg cggacaaact gataaacaat acgactgggt
     7201 aacgcaattt gaaaaagaga caggctgcgc ggtgaatgtg aaaaccgccg cgacttccga
     7261 tgaaatggtc agtctgatga ccaaaggggg ttacgatctg gttacggcat ccggcgatgc
     7321 ctcgctgcgt ttgattatgg gcaaacgcgt gcagccgatt aataccgcct tgattcccaa
     7381 ctggaaaacg ctcgatccgc gcgtggttaa aggcgactgg tttaatgttg gcggcaaagt
     7441 ttacggcaca ccttaccaat gggggccgaa cctgctgatg tacaacacta aaaccttccc
     7501 gacgccgccg gatagctggc aagtggtttt tgttgagcaa aatctgccgg acggcaagag
     7561 caataaaggc cgcgttcagg cttatgatgg ccctatctac attgcggacg ctgcgttgtt
     7621 cgttaaagcc actcagccgc agttgggcat cagcgatccg tatcaactaa ccgaagaaca
     7681 gtaccaggcg gtgctgaaag tgctgcgcgc tcaacacagt ttgatccatc gctactggca
     7741 tgacactacc gtgcaaatga gcgatttcaa aaacgagggt gtggttgctt ccagtgcctg
     7801 gccctatcag gccaacgccc tgaaagccga aggccagcct gttgctaccg ttttcccgaa
     7861 ggagggtgtt accggttggg ctgataccac catgctgcat agcgaagcga aacatccggt
     7921 ttgcgcctac aaatggatga actggtcatt aacgccaaaa gtgcagggcg atgtggcggc
     7981 ctggtttggc tcgttaccgg tagtgccgga agggtgtaaa gccagtccgt tattaggcga
     8041 aaaaggttgt gaaaccaacg gttttaacta tttcgacaaa atcgccttct ggaaaacgcc
     8101 tatagcagaa gggggcaagt ttgttcccta cagtcgctgg acgcaggatt acattgccat
     8161 tatgggcggt cgctaacttc gctggggtgc tttatgacgt acgcagtgga gtttgacaac
     8221 gtctcgcggt tgtacggtga cgtgcgcgca gtagatggcg tcagtattgc gataaaagat
     8281 ggtgagttct tctctatgct ggggccgtcc ggctccggca aaaccacctg cctgcgcctg
     8341 attgctggct tcgaacagct ttccggcggg gctatctcta tctttggtaa acccgccagc
     8401 aatctgccac cgtgggagcg ggacgtgaat actgtctttc aggactacgc gctatttccg
     8461 catatgtcga ttcttgacaa tgtcgcctat gggctgatgg tcaaaggcgt gaataaaaag
     8521 cagcggcacg caatggcgca agaggcgctg gagaaagtgg cgttggggtt tgtacatcaa
     8581 cgtaaaccgt cacaactttc tggtggtcag cgccagcggg ttgctatcgc cagagcattg
     8641 gtgaatgaac cgcgcgtatt gctgttggat gaaccgctcg gcgcactgga tctcaaattg
     8701 cgtgagcaga tgcagctgga actgaaaaaa ctgcaacagt ctctcggtat cacttttatc
     8761 ttcgttaccc acgatcaggg cgaagcgtta tcgatgtccg atcgtgtggc ggttttcaat
     8821 aatggacgca ttgagcaggt cgattccccg cgcgatctct atatgcgccc gcgcacgccg
     8881 tttgttgccg gattcgtcgg gacatccaac gtttttgatg gactgatggc agagaaactt
     8941 tgtggcatga cgggaagctt cgccctgcga ccggaacata tccgcctcaa cacccctggt
     9001 gaactgcagg ccaatggcac gatccaggcg gtgcaatatc agggcgcggc aactcgtttt
     9061 gaactgaaat tgaacggcgg tgaaaaactg cttgtgagtc aggccaatat gacaggcgaa
     9121 gaactgcctg ccacgctcac gcccggacaa caggtgatgg tttcctggtc gcgtgatgtg
     9181 atggtgccgc tggttgagga gaggtgaatg gcgatgaatg tattgcaatc accttcacgt
     9241 ccaggtctgg gtaaggtgtc cggtttcttc tggcgtaatc cggggctggg gctgttttta
     9301 ctgctgcttg gcccgctaat gtggtttggc attgtctatt tcggctcgct gctgacactg
     9361 ttatggcagg gattttatac ttttgacgat ttcaccatgt cggtaacgcc ggaactgacg
     9421 ctggcgaata tccgtgcgct gtttaatccg gcgaattacg acatcattct ccgcacgctg
     9481 actatggctg tggcagtcac tatcgccagc gccattctgg cttttccaat ggcgtggtat
     9541 atggcgcgct ataccagcgg gaaaatgaaa gctttttttt atattgcggt aatgttgccg
     9601 atgtgggcga gctacattgt taaagcctat gcctggacgt tattgctggc aaaagatggc
     9661 gtggctcagt ggtttttaca acatcttggg ctggaaccac tgctgactgc gttccttaca
     9721 ttacctgcgg tgggcggaaa tacgctgtca acttccgggc tggggcgctt tctggtgttt
     9781 ctctatatct ggttgccgtt catgatcctg cccgttcagg cggcgcttga gcgtttgccg
     9841 ccgtcattgt tgcaggcgtc ggctgatctc ggcgcacgtc cacgacaaac ctttcgctat
     9901 gtggtgctgc cgctggcaat cccgggtatt gccgctggct ctatctttac cttctcactc
     9961 acactgggcg attttatcgt cccgcagctg gttggtcctc caggatattt tatcggcaat
    10021 atggtttatt cccagcaggg ggcgattggc a
//
LOCUS       DAMZET010000155        10002 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.155, whole genome shotgun sequence.
ACCESSION   DAMZET010000155 DAMZET010000000
VERSION     DAMZET010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.155"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(46..2154)
                     /locus_tag="POB34_003803"
                     /pseudo
     CDS             complement(46..2154)
                     /locus_tag="POB34_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            2272..3465
                     /locus_tag="POB34_003804"
     CDS             2272..3465
                     /locus_tag="POB34_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDI5920669.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAQEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVSTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(3529..4782)
                     /gene="glyA"
                     /locus_tag="POB34_003805"
     CDS             complement(3529..4782)
                     /gene="glyA"
                     /locus_tag="POB34_003805"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDI5920670.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            5111..6301
                     /gene="hmpA"
                     /locus_tag="POB34_003806"
     CDS             5111..6301
                     /gene="hmpA"
                     /locus_tag="POB34_003806"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HDI5920671.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(6346..6684)
                     /gene="glnB"
                     /locus_tag="POB34_003807"
     CDS             complement(6346..6684)
                     /gene="glnB"
                     /locus_tag="POB34_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HDI5920672.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(6745..8079)
                     /gene="glrR"
                     /locus_tag="POB34_003808"
     CDS             complement(6745..8079)
                     /gene="glrR"
                     /locus_tag="POB34_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HDI5920673.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(8069..8782)
                     /gene="qseG"
                     /locus_tag="POB34_003809"
     CDS             complement(8069..8782)
                     /gene="qseG"
                     /locus_tag="POB34_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HDI5920674.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(8947..>10002)
                     /gene="qseE"
                     /locus_tag="POB34_003810"
     CDS             complement(8947..>10002)
                     /gene="qseE"
                     /locus_tag="POB34_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HDI5920675.1"
                     /translation="QCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSRGQQLQREI
                     AERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLGNSVSFSGP
                     SELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLADQVVGPLT
                     PEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETVVSAHSLPA
                     RAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSSHGARVYID
                     VINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQGELYLVDES
                     GQDVCFRIELPSSKNTK"
BASE COUNT         2355 a         2673 c         2599 g         2375 t
ORIGIN      
        1 aatggctttt gcgaccagtt cgccacgttc ggctttcggc agctagctgg cttgcaggta
       61 aagcggcagc gtgcgctggc agtccagtgc gttcagcatc tctgccgcca gggtgggcat
      121 tccccagtta atcagttgcc cggcggtcat cagggcgtta acgtcgcgtc cctgacacag
      181 accgcgccac tgcgcgagag ctgattcgct acgaccatcg aaccagttca gccaccacag
      241 agtggcgttc agcggataat cgcgcagcag tttctcgcgc tgcacacgcg cgttgtcctg
      301 acgaccactt aacaccagca gcagattatg caggcaaagc acttcctgat tggttgggca
      361 ggcgcgaaga ctttgttggc aaaaatccag accagcgtcg aagttaccat tacgcgccgc
      421 cagtcgtgcc aggccataat agccaccggc tttgctgttg ccgctccaga ccgcacgcca
      481 gaaatcctct tcggcttgtt gatattgtcc ctgacgttcg taagcactgg cgcgaatcaa
      541 actcgcctgt ccgcactgcg gatttttgtt cagcgcatgt gcgcgtttca gagcctgagt
      601 ggcatacgcc accgcttgcg ggaaatctgc gcggttatat tccagcatcg ccagcgccag
      661 gttacagcga taatccagcg ggtccagcgc cacgccgcgc aggtagtaat cgaacggtga
      721 acggctggcg tgatgatatt gctccagatg ctgaccgata aaccaggctt catctgtact
      781 ggtaatgtct tgtgctgcca gtggcgcttt ggcgacgtcc ggcaacggca actcttgcgg
      841 ctgatgttcc tgataactca ggacaatatt gccgtcggca tcggagagct caatggtcag
      901 cctttcagga ttgataccgt gcaacacgcc ctggatggcg gtcgcaggca tcagtgccac
      961 ggcatcatca agtaacgcgt tgcatttgcc gatttcgcgg atcgccaggc ggtatccgtt
     1021 caacggagag atggcataca gcccccactc aatcccccgc tcactacgct ggagttttat
     1081 caccgcatcg cgggaggcat tttgcaccat gcccaaagaa tgataaggca ggaaatactg
     1141 ctcaaaacgc ttctcttcgt aagcatcaag ccaggtaaaa tcaggctggt tatcggcaaa
     1201 aataccggtc atcagttcga tatacgggcc gttattgtcg gtcagactct tgtcccacgc
     1261 ctggccgaat tcactgtgtc cccagctcca ctgtttttta cctggcgcga tatggtggtt
     1321 ggcaacgtgt agcaaaccgc catcttcatc gtgacaccac gcgccaacaa aatcgtactg
     1381 tgatttttca gccatatatg aggttggaac gggcacattt ttatagcgag aaatgtccac
     1441 tccggcggag tagtccactt tgtagtaagt gccggtggcg atggggaaag cggagacggc
     1501 ccgtttgccg tgatcaaaca ccgccgttac atccggcggg aagacgctct gatgcccttc
     1561 cccccctttc actgccgggt tggcccacca caagaaatga cgcggcgtgg cgttgccgtt
     1621 atagacgcgg ctggcgattt ccaacgccgc ccgttcagga cgcagtgtga aacctgtcat
     1681 cacctgtaaa ccgtgcatcg gctccgtttc gccgacccac accgtctgtg cgccgtcttc
     1741 atgggcttcg agggtgaaat caacgggcat aaaggtggtc gggcgatggt gttgcggcca
     1801 gttaaactca atcccgccag agatccacgg tcccagcagc cccaccagcg caggtttaat
     1861 gacttcattg tgatagacaa aatcgcgttg tttcacttta tcccatgcgc gatgcacacg
     1921 accgcccagt tccggcagga tcatcacttt gatgtagtcg ttttccagcc acaccgcctg
     1981 ccaggatttc agggttttct gctcgctcag cgtatcggtc acgccgtagg gataaaccgc
     2041 gccggacgat ccctgataaa cgcgattttc gaggaacatg ggatgtatat cctgcggccc
     2101 ggtttcatag gtcgggatct caacgcgctc ttgccacact tttactggag tcatggtgcc
     2161 ctcaatatta acaagacata ctgaattaaa agatttgtgg cagtatattg aacaatatgg
     2221 caatgttttc gcggaataat cacgcaatta actaaacaag gtttagtgaa gatgagagcc
     2281 tgcattaaca atcaacagat tcgccaccat aacaaatgcg tgattctgga actgctgtac
     2341 cggcaaaagc gcgccaataa atcaacgctg gcccggctgg cgcaaatttc gattccggca
     2401 gtcagtaata ttttgcagga actggaaagc gaaaaacggg tggtgaatat cgacgatgaa
     2461 agccagacgc gcgggcatag tagcggtaca tggctgattg cgcaggaagg tgactggacg
     2521 ctgtgcctga acgtgacgcc caccagtatt gagtgtcagg tggctaatgc ttgtttaagt
     2581 ccgaaaggcg agtttgagta tttacagatt gatgcaccga cgccgcaggc gctgctgtcc
     2641 gaaatcgaaa aatgctggca tcgccaccgt aaattgtggc cggaccgcac catcaatctg
     2701 gcgctggcaa tccacggtca ggttgatcct gtgaccggcg tgtcgcaaac catgccgcaa
     2761 gcgccgtgga caacgccggt ggaggtgaaa tatctgctgg aagagaagct cggcattcgg
     2821 gtgatggtcg ataatgactg cgtgatgctg gcgctggcgg agaaatggca aaataattcg
     2881 caggaacggg atttctgcgt gatcaacgtt gattacggca ttggctcgtc gttcgtgatt
     2941 aacgagcaaa tttatcgcgg cagtttgtat ggtagcggac agattggtca caccatcgtt
     3001 aatccggatg gcgtcgtctg cgactgtgga cgttatggct gcctggaaac cgtcgcctcg
     3061 ttaagcgcat taaaaaaaca ggcgcgggta tggctaaaat cacaaccggt tagtactcaa
     3121 cttgatcctg aaaaactgac tacagcgcag ttaatcgctg cctggcaaag tggagaaccg
     3181 tggatcacca gctgggttga ccgcagtgcc aatgccattg gtttgagtct gtataacttc
     3241 ctcaacatcc tcaatattaa tcagatttgg ttgtacggtc gcagttgtgc ctttggtgag
     3301 aactggctta atactattat tcgccagaca ggatttaacc cgttcgaccg cgacgaagga
     3361 ccgagcgtga aagcgacgca aattggcttc gggcaattaa gccgcgcaca acaggtgctg
     3421 ggaattggct atttgtatgt tgaggcgcag ttacggcaga tttgatggcg caataacgta
     3481 gaaaggcttc ccgaaggaag ccttttttca acagattaac cgtttcgctt atgcgtaaac
     3541 cggataacgt gcgcagatgt cgagaacttt acctttgatg cgctcgataa cagcttcatc
     3601 attgatgctg tccagcacgt cacacatcca gccagccagt tctttcgctt ctgcttcttt
     3661 gaagccgcga cgcgtaattg ccggagtacc cacgcgaata ccggaggtca caaacgggct
     3721 cttcggatcg ttcggtacgc tgtttttgtt gacggtgatg ttagcacggc ccagagcggc
     3781 gtctgcttct ttaccggtca ggtttttatc aaccagatca accaggaaca ggtggttatc
     3841 agtgccgccg gaaaccactt tgtagccgcg ttccaggaac acttctacca tcgctttagc
     3901 gtttttagcg acctgctgct ggtaagtttt gaactcaggc tccatcgctt ctttcagagc
     3961 aaccgcttta ccagcgatta catgcatcaa cggaccgccc tgaccaccag ggaaaacggc
     4021 agagttcagt tttttgtaca gctcttcgct accacctttc gccaggatca gaccgccgcg
     4081 cggacccgcc agggttttgt gagtggtggt agtaacaacg tgagcatgag gaaccgggtt
     4141 cgggtagacg ccagcagcaa ccaggcccgc aacgtgcgcc atatcaacga acaggtaagc
     4201 accgatgctg tcagcgattt cacgcatttt cgcccagtcc accacgccgg aatatgcaga
     4261 gaaaccaccg ataatcattt tcggcttgtg ttctttggct tgtttttcca gatcggcgta
     4321 gtcgatatga ccggtagcat cgataccgta aggaacgatg ttgtacagtt taccggagaa
     4381 gttaaccgga gaaccgtgag tcaggtgacc gccatgcgcc aggttcatac ccagaacggt
     4441 atcacctggt tccagcagcg cggtgtagac cgcaaagtta gcctgggagc cggagtgcgg
     4501 ctggacgtta gcgtagtcag cgccgaacag ttctttcgca cgatcgatcg ccagttgttc
     4561 aacgatatca acatactcgc aaccgccgta gtagcgtttg cccggataac cttcagcata
     4621 tttgttggtc agctgagaac cctgcgcctg cattacgcgc gggctggtgt agttttcgga
     4681 ggcgatcagt tcgatgtgct cttcctgacg tactttttcc tgctccatag cctgccacag
     4741 ttcggcatca taatcggcaa tgttcatttc acgctttaac atccgcatct cctgactcag
     4801 ctaacaataa aatttttggc ctttataggc ggtcctgttg gacaacggcg aacagtataa
     4861 ccgaatcatt gtgcgataac aggtcttgac aaaggaattt acgcaaacga ttaccttcag
     4921 gctacgcaag gctttggaga ataaagagct tgcaaccgga aacggatttc ttttcaggtt
     4981 tgtgatgcaa atttttcact tcatcacatt ctttctgaaa aacaccaaag aaccatttac
     5041 attgcagggc tattttttat aagatgcatt tgagatacat caattaagat gcaaaaaaaa
     5101 ggaagaccat atgcttgacg ctcaaaccat cgctacagta aaagccacca tccctttact
     5161 ggtggaaacg gggccaaagt taaccgccca tttctacgac cgtatgttta ctcataaccc
     5221 agaactcaaa gaaattttta acatgagtaa ccagcgtaat ggcgatcaac gtgaagccct
     5281 gtttaacgct attgccgcct acgccagtaa tattgaaaac ctgcctgcgc tgctgccagc
     5341 ggtagaaaaa atcgcgcaga agcacaccag cttccagatc aaaccggaac agtacaacat
     5401 cgtcggtgaa cacctgttgg caacgctgga cgaaatgttc agcccggggc aggaagtgct
     5461 ggacgcgtgg ggtaaagcct atggtgtact ggctaatgta tttatcaatc gcgaggcgga
     5521 aatctataac gaaaacgcca gcaaagccgg tggttgggaa ggtactcgcg atttccgcat
     5581 tgtggctaaa acaccgcgca gcgcgcttat caccagcttc gaactggagc cggtcgacgg
     5641 tggcgcagtg gcagaatacc gtccggggca atatctcggc gtctggctga agccggaagg
     5701 tttcccgcat caggaaattc gtcagtactc tttgactcgc aaaccggatg gcaaaggcta
     5761 tcgtattgcg gtgaaacgcg aagagggtgg gcaggtatcc aactggttgc acaatcacgc
     5821 caatgttggc gatgtcgtga aactggtcgc tccggcaggt gatttcttta tggctgtcgc
     5881 agatgacaca ccagtgacgt taatctctgc cggtgttggt caaacgccaa tgctggcaat
     5941 gctcgacacg ctggcaaaag caggccacac agcacaagtg aactggttcc atgcggcaga
     6001 aaatggcgat gttcacgcct ttgctgatga agttaaggaa ctggggcagt cactgccgcg
     6061 ctttaccgcg cacacctggt atcgtcagcc gagcgaagcc gatcgcgcta aaggtcagtt
     6121 tgatagcgaa ggtctgatgg atttgagcaa actggaaggt gcgttcagcg atccgacaat
     6181 gcagttctat ctctgcggcc cggttggctt catgcagttt accgcgaaac agttagtgga
     6241 tctgggcgtg aagcaggaaa acattcatta cgaatgcttt ggcccgcata aggtgctgta
     6301 atttgatgtt gccggatgga aacatccggc aacccttgac gcggtttaaa ttgccgcgtc
     6361 gtcctcttca ccggtacgga tgcgaatgac ccgtgccacg tcaaagacga agattttacc
     6421 gtcaccgatt ttgccggttt gcgccgtgcg aataatggta tcgacacagg tatcgacaat
     6481 gtcgtccggt acgacaatct caattttcac tttcggcaga aaatccacca tatactccgc
     6541 gccgcggtac agctcggtat ggcctttctg gcgaccaaag cctttcactt cggtcaccgt
     6601 catgccggta ataccgactt cggccagtgc ttcgcggaca tcgtccagct tgaagggttt
     6661 tataatcgca tcaatctttt tcatgctatt ccttgaaaag gtcgcctgtc ttttgatctg
     6721 ctaaacgtaa cacataacgc caattcattc cttgaaatcg tttgcatcca gctcgtgtcg
     6781 ggaaagcagt ttataaaatt ctgtccggtt gcgccccgcc attctcgccg cgtgggtgac
     6841 gttgcctttg gtgatttgca gcagcttacg caaatagttg agttcaaact gattacgtgc
     6901 ctcaacaaag gttggcagcg ccgtgttttc accctccagc gcctgctcca ccagcgcatc
     6961 actaatcacc ggagatgagg tcagcgccac gcactgttca atcacgttga ccaactggcg
     7021 cacattaccc ggccagctcg cggtcatcag gcgtttcatc gcatcggtag agaacgcgcg
     7081 gacaaacggt ttatgtcgct ctgccgcctg gcgcaacagg tgatttgcca gtagcggaat
     7141 gtcttctgtg cgctccgcca gtgccggaat tttcaggctg acaacgttga ggcggtaata
     7201 gagatcttcg cggaattccc cgcgcgccat cgcttttggc aggtcgcggt gagtggcgga
     7261 aataatccgc acgtcgatat caatatcgcg gttactgccc agcgggcgca ctttacgctc
     7321 ctgcaacacg cgcagcagtt tgacctgcaa cggtgcgggc atatcgccaa tctcatcgag
     7381 aaatagcgta ccgccttccg ccgcctggaa taaaccttcg cgattgctga cagcgccagt
     7441 aaacgcgcca cgcgcatgac caaacagctc cgactccagc aattgctcag gcaatgcacc
     7501 acagttaata gcaataaatg gtttgctatt gcgcgggctg gcgttgtgga tagcctgggc
     7561 gaaaatctct ttcccagtgc cgctctggcc attaatcaaa acgctgacgt ctgattgcgc
     7621 caccagccgc gcctgttcaa gcaaacgcag catcagcggg ctgcgggtga caatcgcctc
     7681 gcgccagcgt tcgtcggtgg ctggcgcgga ttgttccagc gcatcatcaa ttgcctgata
     7741 tagcgcatct ttatcgacag gcttggtgag gaaactaaaa acgccctgct gtgttgcagc
     7801 aacggcatcg gggatagaac catgcgcagt aagaataatt actggcattc caggctgcac
     7861 tttctggatt tcagcaaaca gctgcatacc gtccatttca tccatccgca ggtcgctgat
     7921 aactaaatct actttttcgc gattcagtac ccgtaatcct tcagcaccac tctccgccgt
     7981 gaccacactg tagccttcgc tggtcaggcg caggccaagc agtttcagca atcccggatc
     8041 gtcatcgacc aataataaat gcgcaggttt atggctcatc aggagtgacc tcatgggtgg
     8101 atggcgcggg cttttcactc tcgtgcggcg tatccggcga gaaatttcca gccggtttgc
     8161 gggtcgagag ctggcgttca atatcggtca ggttttccag cttgcgggtg gtgagttcca
     8221 gttgctgctg tagaacgtga tgttgctggc gcaatgtatc cagctcgctg tcgctggact
     8281 gctggagttt actgtaacgt tggcgctctt ccgccagttg cagttgcagc gcctgaccgt
     8341 cgcgccagag ttgatacagt gggcgaacct gtgctgggat ctcggtactt aacgcttcaa
     8401 tacgcgcgac cagctggcgg cgctcatacg gcgtaatttt ggcgtcggcg agcaaaatcc
     8461 cttgtttaaa ggtattttgc cagctgccgt cgtcatattg gcgagcttgc tgacgcgact
     8521 gcgcaggcat taaacgatca gcacaatcca tcgcccgcag ccagtaaagc ggattggttt
     8581 cggttgattt accttgcagc gcccagatgt cgctacattc agtagaaaga tagtcagcca
     8641 gttgataaac cggaattttt tcttctgctg gcgtatcaat ggctggctta ttgtgattct
     8701 gcacgcaacc cagcaatgcc agacatggca gccctgccag ccacagccgt cggggcaata
     8761 atcgttgaaa aatgtgtcgc atattcacca gacttaaagc ctatctcagt gggcgtaatt
     8821 gttgcagaca gtctggacat ggacagcgcg gagaaaccgg agcgtacata tcgtacgtga
     8881 agatttcgag cactgcccgg ggccgaactg acaaataaaa tagcctgata ggataggctg
     8941 ttagcattat ttcgtgtttt tcgacgacgg taattcaatg cggaaacaaa cgtcttgccc
     9001 gctctcgtcg accagataca gttccccttg catacggcga atacaatccc tggcaatgct
     9061 taatcccaga ccgctgccct tcaccgcccc ttttcgctgg tggcttccct gaaaaaaggg
     9121 ttcgaagatc atggcgcgtt cctcttgcgg aatgggcgtg cctgtattga tgacatcaat
     9181 ataaacccgc gcaccatgtg aactgctgcg aaggcaaatg ttaccggatt cagccccgta
     9241 gtgcaccgca ttggagtaaa gattatccag tacgctcatc agcagcattg gctccgccag
     9301 gcaagctgtt gctttgagat cgacgtcggt atgcatcatt ttagcccgtg cgggcaggct
     9361 atgagcagaa accactgtct ccaccagcgg tgctaattca acattctcca gttccaccgc
     9421 actgtccgcc tgtttacggt tgtaatcaag cagttgttcg atcagttttt gcaaattgcg
     9481 gctgctgcta tcaagaatgc tcaccacctc tttttgctct ggcgtaagcg gcccgacaac
     9541 ctggtcagcc agtaattcag tgccctcgcg catactcgcc agtggcgttt ttaattcatg
     9601 agataaatgt cttaaaaatt gatggcgttg ggattccagc catgacaggc gctcacttaa
     9661 ccaaagaata cgttgcccaa ccgagcgtaa ctcgctcggt ccactgaacg aaacgctatt
     9721 gcccagagaa cgcccttccc ccagacggtt gatcatgcgc tcgatatttt tcaccggccc
     9781 gataatcatc cgcgtgaaaa gtagcaccat caccagactc accagaaata gcaccagcga
     9841 ttgccaacca aaatattgcc cacgttcggc gatttcacgc tgaagttgct gcccacgaga
     9901 gaacaccact gtgcgcgtgg cctgcaccat ttcggtattg gcactggcaa aggcttcaag
     9961 acgcgcggcg gcggcagcat cgggaccact gttgttacac tg
//
LOCUS       DAMZET010000156         9889 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.156, whole genome shotgun sequence.
ACCESSION   DAMZET010000156 DAMZET010000000
VERSION     DAMZET010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.156"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..398
                     /locus_tag="POB34_003811"
     CDS             <1..398
                     /locus_tag="POB34_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HDI5920676.1"
                     /translation="PVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVENMSVHI
                     CSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRPESEFTA
                     IYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            661..942
                     /locus_tag="POB34_003812"
     CDS             661..942
                     /locus_tag="POB34_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HDI5920677.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDARHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            1235..1777
                     /locus_tag="POB34_003813"
     CDS             1235..1777
                     /locus_tag="POB34_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI5920678.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKFSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            1857..2531
                     /locus_tag="POB34_003814"
     CDS             1857..2531
                     /locus_tag="POB34_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDI5920679.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            2547..5027
                     /locus_tag="POB34_003815"
     CDS             2547..5027
                     /locus_tag="POB34_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDI5920680.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKHSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     STPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            5043..6077
                     /locus_tag="POB34_003816"
     CDS             5043..6077
                     /locus_tag="POB34_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HDI5920681.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKKEDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSPVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKIKSGALE
                     ATALINVKYD"
     gene            complement(6159..6497)
                     /gene="rcnB"
                     /locus_tag="POB34_003817"
     CDS             complement(6159..6497)
                     /gene="rcnB"
                     /locus_tag="POB34_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HDI5920682.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(6716..7540)
                     /gene="rcnA"
                     /locus_tag="POB34_003818"
     CDS             complement(6716..7540)
                     /gene="rcnA"
                     /locus_tag="POB34_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HDI5920683.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            7661..7933
                     /gene="rcnR"
                     /locus_tag="POB34_003819"
     CDS             7661..7933
                     /gene="rcnR"
                     /locus_tag="POB34_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HDI5920684.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      8025..8121
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8156..8944
                     /gene="thiM"
                     /locus_tag="POB34_003820"
     CDS             8156..8944
                     /gene="thiM"
                     /locus_tag="POB34_003820"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDI5920685.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            8941..9741
                     /gene="thiD"
                     /locus_tag="POB34_003821"
     CDS             8941..9741
                     /gene="thiD"
                     /locus_tag="POB34_003821"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDI5920686.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            9806..>9889
                     /locus_tag="POB34_003822"
     CDS             9806..>9889
                     /locus_tag="POB34_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HDI5920687.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYP"
BASE COUNT         2741 a         2110 c         2379 g         2659 t
ORIGIN      
        1 ttccagtaac tggtgcagtc gtggtcacca ccccgcagga catcgcgttg atcgatgcga
       61 aaaaaggcat tgtgatgttc gagaaagtcg aggttccggt gcttggtatt gtcgagaaca
      121 tgagtgtgca tatttgcagt aactgtggtc accatgaacc gattttcggt accggtggtg
      181 cagagaaact ggctgaaaaa taccacacac agctgctggg gcagatgccg ttacacattt
      241 ccttacgtga ggatctcgat aaaggcacgc caacggttat cagccgtccg gagagcgaat
      301 ttaccgctat ctaccgccag ctggctgacc gcgttgcagc tcagctctac tggcagggtg
      361 aagtcattcc aggcgagatt tccttccgcg cggtctaacg cctattaacc attctggtta
      421 ttcttcatac accgcagaga tgttacattg atgcggtgta ttagtttttg ccgcattaat
      481 gaacacttat ttaatactgc gaatattctg cggaccttcg tccgaaactc tacaaagtcg
      541 attgatcata caggcaatgc ttcattatca tccttcgtgg ctgtgtagtg gatgtacagc
      601 tatagtttgt tatgcactca ttctatccag gcataacctt tactacaggg ataacgttca
      661 atgaataagt attggctgtc aggaattatt tttcttgcct atggattggc ttctcccgca
      721 ttctcttcag agactgccac attaacgatt aatggcagga tcagtccccc aacatgcagt
      781 atggctatgg ttaatagtca accacaacag cattgtggtc agttaacgta taatgttgac
      841 gcccgtcatc aggtttcttc gcccgtcaaa ggtgttacga cagaagtggt tgttgcaggt
      901 agcgatagca aacgtcgaat cgtgttaaat cgctacgatt aatttggcaa cctgatttta
      961 attgcattta gaaagccgaa atcatttata taaatgatat cggctttttt attgatatca
     1021 acaataccat ttacataaat ctacattaat taatagatgc gataaatatt taatactctt
     1081 aagtatttgt tgcatttata ttgcattggt taatttgttt taaatcaata aaacaatatg
     1141 tttacattgc ggcaactttt ttgatgtgag aaatttgttt ccaagagata cagactctta
     1201 acaaccaaaa ataaatttta ttaaggaaca tattatgaaa cgttcaatta ttgctgccgc
     1261 tgtcttttct tcttttttta tgagcgctgg agtatttgct gcagacgttg ataccggaac
     1321 attaaccatt aaaggaaata ttgcagaatc tccgtgtaaa ttcgaagcgg gtggtgattc
     1381 agtaagtatt aatatgccga ctgtaccaac cactgtcttt gaaggtaaag ctaaatattc
     1441 tacctatgat gatgcagtcg gtgtaaccag cagcatgtta aaaattagct gcccgaaaga
     1501 agttgctggt gtaaaattct cgttgattac caacgacaaa ataaccggca acgataaggc
     1561 gatagccagt agcaacgata ccgtgggtta ctatctctat ttaggtgata acagcgatgt
     1621 cctggatgtt tctgcacctt ttaatattga gagttataaa acagcggaag gtcaatatgc
     1681 tattccgttt aaagcaaaat acctgaaact aacagataac tcagtgcaat caggtgatgt
     1741 gttatcttct ctggttatgc gtgtggcgca ggattaataa ataaaattgc aagtttctcc
     1801 aggtaataaa catggccgct atcccatggc ggccttttaa tttaagaggc attaaaatga
     1861 aaggattatt atctttactc attttttcta tggtccttcc tgcacatgcc ggaattgtta
     1921 tctacgggac gcgcattatt tacccggcag aaaataaaga agttatggtg cagttgatga
     1981 accagggaaa tcgttcttcg ctgctgcagg cgtggattga tgatggcgat acgtcgttac
     2041 caccagaaaa aattcaggtt cctttcatgt taacgccacc agttgcaaaa ataggggcaa
     2101 attccgggca gcaagtaaaa atcaaaatta tgccgaataa actgcccact aataaagaaa
     2161 gcatttttta tctgaatgtt ctggatattc caccaaatag tccagaacaa gaaggtaaga
     2221 atgcactgaa gtttgcgatg caaaacagaa tcaaattgtt ttaccggccg gcgggtattg
     2281 ctccggtaaa taaagcgacc tttaaaaagt tactggtaaa tcgcagtggc aatggattgg
     2341 tgataaaaaa tgactcagct aattgggtga cgatttcgga tgtcaaagct aataatgtca
     2401 aagtcaatta tgaaactata atgattgccc ccttagaaag tcagagtgtt aatgtcaaaa
     2461 gtaataatgc aaataactgg tatctgacca ttatcgatga ccacggcaac tatattagtg
     2521 acaaaattta attgcaggag ctgcctatgt tgagaatgac cccacttgca tcagcaatcg
     2581 tagcgttatt gctcggcatt gaagcttatg cagctgaaga aacctttgat acccatttta
     2641 tgataggtgg aatgaaagac cagcaggttg caaatattcg tcttgatgat aatcaaccct
     2701 taccggggca gtatgacatt gatatttatg tcaataagca atggcgcggg aaatatgaga
     2761 ttattgttaa agacaacccg caagaaacat gtttatcaag agaagttatc aagcggttag
     2821 gcattaatag cgataacttc gccagcggta agcaatgttt aacatttgag caacttgttc
     2881 agggtgggag ctatacctgg gatatcgggg tttttcgtct cgatttcagt gtcccgcagg
     2941 cctgggtgga agaactggaa agtggctatg ttccaccgga aaactgggag cggggtatta
     3001 atgcgtttta tacctcttat tatgtgagtc agtattacag tgactataaa gcgtcgggta
     3061 atagtaagag tacatatgta cgttttaaca gcgggttaaa tttactgggg tggcaactgc
     3121 attctgatgc cagtttcagt aaaacaaata acaatccagg ggtgtggaaa agcaataccc
     3181 tgtatctgga acgtggattt gcccaacttc tcggcacgct tcgcgtgggt gatatgtaca
     3241 catcaagcga tatttttgat tctgttcgct tcagaggtgt gcggttgttt cgtgatatgc
     3301 agatgttgcc taactcgaaa caaaatttta cgccacgggt gcaggggatt gctcagagta
     3361 acgcgctggt aactattgaa cagaatggtt ttgtggttta tcagaaagag gttcctcctg
     3421 gcccgtttgc gattacagat ttgcagttgg ccggtggtgg agcagatctt gatgtcagcg
     3481 tgaaagaggc ggacggttcg gtaaccacct atctggtgcc ttatgcagcg gtgccaaata
     3541 tgctgcaacc cggcgtgtcg aaatatgatt ttgcggcggg tcgtagccat attgaagggg
     3601 cgagcaaaca tagtgatttt gtccaggcgg gttatcagta tggttttaat aacttattga
     3661 cgttgtatgg tggctcgatg gttgcgaata attattacgc gttcactttg gggactggct
     3721 ggaatacacg cattggtgcc atttccgtcg atgccacgaa gtcgcatagt aaacaagaca
     3781 acggcgatgt gtttgacggg caaagttatc aaattgccta caacaaattt gtgagccaaa
     3841 cgtcgacgcg ttttggtctg gcggcctggc gttattcgtc gcgtgattac cggacattta
     3901 acgatcacgt ttgggcaaac aataaagata attatcgccg tgatgaaaac gatgtctatg
     3961 acattgccga ttattaccag aatgattttg gtcgcaaaaa tagcttttcc gccaatatga
     4021 gccagtcatt gccagaaggt tgggggtctg tgtcattaag tacgttatgg cgagattact
     4081 gggggcgtag cggcagtagt aaggattatc agttgagtta ttccaacaac ctgcgacgga
     4141 taagctatac cctcgcggca agccaggctt atgacgagaa tcatcatgaa gagaaacgtt
     4201 ttaatatttt tatatcgatt ccctttgatt ggggtgatga cgtttcgacg cctcgtcggc
     4261 aaatatatat gtctaactca acgacgtttg atgatcaggg gtttgcctca aataatacgg
     4321 gattatcagg aacagtaggg agtcgggatc agttcaatta tggtgtcaac ctgagtcatc
     4381 aacatcaggg aaatgaaacg acagctggag cgaatttaac ctggaacgcg ccggtggcaa
     4441 cagtgaatgg cagttatagt cagtcgagta cttatcgaca ggctggagcc agtgtttcag
     4501 ggggcattgt cgcctggtcg ggtggcgtta atctggcgaa ccgtctttcc gaaacgtttg
     4561 ctgtgatgaa tgcgccagga attaaagatg cttatgtcaa tgggcaaaaa tatcgcacaa
     4621 caaaccgtaa tggagtggtg gtatacgacg gaatgacacc ttatcgggaa aatcacctga
     4681 tgctggatgt gtcgcaaagc gatagcgaag cagaattacg tggcaaccgg aaaattgccg
     4741 caccttatcg cggcgcggtt gtactggtta attttgatac cgatcagcgc aagccctggt
     4801 ttataaaagc gttaagagcg gatgggcaac cattaacgtt tggttatgaa gtcaatgata
     4861 tccacggtca taatattggc gttgtcggcc agggaagcca gttatttatt cgcaccaatg
     4921 aaataccgcc atcggttaat gtagcaattg ataagcaaca aggactttca tgcacaatca
     4981 ccttcggtaa agagattgat gaaagtagaa attatatttg ccagtaatag tcaggtggtt
     5041 ctatggaaat tcgcataatg ctatttatat taatgatgat ggttatgcct gtgagctatg
     5101 cggcatgtta tagtgagtta tctgttcagc acaatttggt tgttcagggg gattttgcac
     5161 ttactcaaac acaaatagcg acatatgagc ataattttaa tgattcgtca tgcgtgagta
     5221 caaatactat cacccctatg agttcgtcgg atattattgt tgggctttat aacgatacca
     5281 taaaattaaa tttacatttt gaatggacca ataaaaacaa catcacgttg tcaaataatc
     5341 agaccagttt caccagtggt tattcagtta cggtgacacc tgcggccagt aatgcaaaag
     5401 tgaatgtttc tgcggggggc ggcggttcag tgatgattaa tggtgttgcg acattatcca
     5461 gtgcttcatc atcgacacgc gggagtgccg cagtacaatt tctactgtgt ttattaggtg
     5521 gcaagtcatg ggatgcatgt gtaaatagct acagaaatgc attggcacaa aatgcaggtg
     5581 tctattcctt taatctgaca ttgtcataca acccgataac cacaacctgc aaaccggacg
     5641 atttattaat tactttagac agtattcccg tttcacaatt accagccaca ggtaacaaag
     5701 caacaataaa tagtaaaaaa gaggatatta ttctgcgttg taaaaattta ttaggtcaac
     5761 aaaatcaaac atcacggaaa atgcaggtgt atttatcaag ttctgacttg ttaaccaaca
     5821 gcaacacaat actgaaaggt gcggaagata atggcgtagg atttattctt gaaagtaatg
     5881 gttcgccagt cacactttta aatatcacta acagcagtaa aggatacaca aatttaaaag
     5941 aaattgcggc aaagtcaaaa cttacagata caacggtttc aattccgata acagccagtt
     6001 actacgtcta cgatacaaat aaaattaaat ctggcgcact ggaggcaacc gcattaatca
     6061 acgtgaaata cgactaataa caagcaagac gagcaagtgg ctaataataa aaaataacaa
     6121 ggtgtctgca tttccccctc atgatgaggg ggcttttttt agcgatgata aaaaatctca
     6181 ccgtcgtagg ctttaatgat tttaccgtcg gtgtcgctga tcaacacgta cgcgccaccc
     6241 atataggtcc agtgcgtccc ggcatcaggc gcgggcaggt tacgtaactg ccactgctta
     6301 atgttgtact catcggtgcg gtacatctct gggacggtat cacctggttt gaaatgctta
     6361 aagtcagcaa tgaaactact taactcatac ttagaaattc ccgaggtatt ggtcgaaccc
     6421 gcggtggctg gtgcggccca ggcggcactg gtaaccagca aaagcacacc cagcaacatc
     6481 ttatttttaa tggtcataaa ctctccactt tggggatctg taatatcagt cgcatatctt
     6541 tcctgccata caatataact gacaagcgat attttgttag tgatcccatg atttcgtaaa
     6601 aagtgtaaat gaaaggatgc cctaaaagca tccttagcga gcagagagtc agcggaaata
     6661 gctgatttaa gaatcatcct gggctgaaga taacatgcaa gcctaaagga ttgagttatc
     6721 gcattatgcc catgaagcca tgtacgccca tatacacacc gactaagcca atcaacagac
     6781 tggaaaaata gggggcgcgt ttagcgagag tgttaaatcc gctccagcgt tttgcgacct
     6841 gctgaacgct gattgctgcg ccaacgccta cggtgacaag cgttaacgcc aggccaatgc
     6901 tgaaactgac gaccagtgtt gcgcccagtg tcagggcttt caactgaatg caaatcaaca
     6961 gcacggtaat tgctgccgga caggggataa gaccacctgt taagccaaat aacaaaattt
     7021 gccagttggt gacctctcta ccatcaaagc gtcgtttaat atcattggca tgggctcgtg
     7081 catgggcatc ctgatactcg ccatgttcgt gatggtgatg atgtccatgg tcgtggtgat
     7141 cttcgtgatc gtggtgatga tgttcatgat catactcgtg catattctcc agccagttgc
     7201 gttcgccgcg ccaggtacgc cagaacatcc agaacgcggt gccaataatg atcactgcgg
     7261 aaatcagctg gagccacggt tctgctgatt gagcagtaaa gcgcttgctg atcaccatcc
     7321 cgccaaaggc aattaaccag accactgcgg tatgcgaaat agttgctgcc agtccgagca
     7381 tcaccgcctg tttaatggtg cctttgatgg cgatgataaa cgccgccatc atcgtttttg
     7441 agtgccctgg ttccaggcca tgaagcgcac caagtaagat ggcgctgggg atgaagaacc
     7501 aggcgtttcc ttgctgaaga agagttgtaa attcggtcat gataataatt cttagtatta
     7561 attcggcaat ctgattctac tcccccccag tacctgatac taccccccag tagattaata
     7621 gtgctatgat ttttcatatt cttgttaacc aggtgttgcc atgtctcata caatccgtga
     7681 taaacagaaa ctgaaagcgc gtgccagtaa gattcagggc caggtcgtgg cgctcaagaa
     7741 aatgctcgac gagccgcacg aatgcgctgc agttttacaa cagattgctg ctatccgtgg
     7801 cgcggtaaac ggtctgatgc gggaagtgat taaaggtcat ctgacggaac acatcgttca
     7861 ccagggggat gagctaaaac gtgaagaaga tctggatgtc gttctgaagg tgctggattc
     7921 atatatcaaa taatttatta acgcgattgt aaaactgccg tttttcctcg tttacaacgc
     7981 gtgcgctgga cattaccatc ctcctctgcg atttatcatc gcaaccaaac gactcggggt
     8041 gcccttctgc gtgaaggctg agaaataccc gtatcacctg atctggataa tgccagcgta
     8101 gggaagtcac ggaccaccag gtcattgctt cttcacgtta tggcaggagc aaactatgca
     8161 agtcgacctg ctgagttcag cgcaatctgc gcacgcgtta cacctttttc accaacattc
     8221 ccctcttgtg cactgcatga ccaacgatgt ggtgcaaacc tttactgcca ataccttgct
     8281 ggcgctcggt gcatcgccag cgatggttat cgaaaccgaa gaggccagtc agtttgcggc
     8341 tatcgccagt gccttgttga ttaacgttgg cacactgacg cagccacgcg ctcaggcgat
     8401 gcgtgctgcc gttgagcaag caaaaagctc tcaaacaccc tggacgcttg atccagtagc
     8461 ggttggtgcg ctcgattatc gccgccattt ttgtcatgaa cttttatctt ttaaaccggc
     8521 agcgatacgt ggtaatgctt cggaaatcat ggcattagct ggcattgcta atggtggacg
     8581 gggagtggat accactgacg ccgcagctaa cgcgataccc gctgcacaaa cactggcacg
     8641 ggaaactggc gcaatcgtcg tggtcactgg cgaggtggat tatgttaccg atggccatcg
     8701 cgccgttggc attcacggtg gcgatccgtt aatgaccaaa gtggtaggaa ctggctgtgc
     8761 attatcggcg gttgtcgctg cctgctgtgc gttaccaggc gatacgctgg aaaatgtcgc
     8821 atctgcctgt cactggatga aacaagccgg agagcgcgca gtcgccagaa gcgaggggcc
     8881 aggcagtttt gttccacatt tccttgatgc gctctggcaa ttgacgcagg aggtgcaggc
     8941 atgaaacgaa ttaacgctct gacgattgcc ggtactgatc ccagtggtgg tgcggggatt
     9001 caggccgatc ttaaaacctt ctcggcactt ggcgcttatg gttgctcagt tattactgca
     9061 ctggtggcgc aaaatacccg tggcgtacag tcggtgtatc gcattgagcc tgattttgtc
     9121 gccgcccagc tcgattcggt gttcagcgat gtgcgaatcg acaccactaa aatcggtatg
     9181 ttggcggaaa ccgatattgt tgaagcggtg gcagaacggt tgcaacgtta tcagatccaa
     9241 aacgtggtac tcgacaccgt tatgctggca aaaagcggcg acccgctgct ttcaccttcg
     9301 gcggttgcta cgctgcgcag tcgattattg ccacaggttt cattaataac gccaaacttg
     9361 cccgaagctg ccgccttgct cgacgcgcca cacgcgcgca ccgaacagga aatgctggaa
     9421 caagggcgat cgctgttggc gatgggctgt ggcgcagtgc taatgaaagg tggtcatctg
     9481 gatgatgagc aaagcccgga ctggctgttt acccgcgagg gtgaacaacg gtttaccgca
     9541 ccgcgcatta tgaccaaaaa cacccacggc actggttgta cgctctctgc ggcgttggct
     9601 gcactacgcc cgcgccatac aaactgggct gacaccgtac aggaggcaaa aagctggctt
     9661 tcatcagcat tagcccaggc cgacacgctg gaagttggtc acggtattgg tccggttcac
     9721 cacttccacg cctggtggtg agcaccgtac tatagctgct ttggttattg tcagcctttg
     9781 gcactgacat tcagggatga aaccaatgca gctaagaatt accagtcgaa aaaaactcac
     9841 ctctctatta tgcgctcttg ggctaatttc catcgttgca atttatccg
//
LOCUS       DAMZET010000157         9774 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.157, whole genome shotgun sequence.
ACCESSION   DAMZET010000157 DAMZET010000000
VERSION     DAMZET010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.157"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..66
                     /locus_tag="POB34_003823"
     CDS             <1..66
                     /locus_tag="POB34_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HDI5920688.1"
                     /translation="SKSVELHDKVIGHYLNIKHYQ"
     gene            complement(83..238)
                     /gene="cvaC"
                     /locus_tag="POB34_003824"
                     /pseudo
     CDS             complement(83..238)
                     /gene="cvaC"
                     /locus_tag="POB34_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V"
     gene            complement(216..452)
                     /gene="cvi"
                     /locus_tag="POB34_003825"
     CDS             complement(216..452)
                     /gene="cvi"
                     /locus_tag="POB34_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V immunity protein"
                     /protein_id="HDI5920689.1"
                     /translation="MDRKRTKLELLFAFIINATAIYIALAIYDCVFRGKDFLSMHTFC
                     FSILMSAICYFVGDNYYSISDKIKRRSYENSDSK"
     gene            complement(916..1197)
                     /locus_tag="POB34_003826"
     CDS             complement(916..1197)
                     /locus_tag="POB34_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920690.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSIAVAEMFHNAAWGVRTLW
                     FELVTKIDIDIHKKNRYASYDLRRKIEMQHEEFQKMTEREQVPLLKSPE"
     gene            1370..1537
                     /locus_tag="POB34_003827"
                     /pseudo
     CDS             1370..1537
                     /locus_tag="POB34_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282376.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-B"
     gene            complement(1555..2082)
                     /gene="cbi"
                     /locus_tag="POB34_003828"
     CDS             complement(1555..2082)
                     /gene="cbi"
                     /locus_tag="POB34_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin B immunity protein"
                     /protein_id="HDI5920691.1"
                     /translation="MTSNKDKNKKANEILYAFSIIGIIPLMAILILRINNPYSQVLYY
                     LYNKVAFLPSITSLHDPVMTALMSNYNKTAPVMGILVFLCTYKTREIIKPVTRKLVVQ
                     SCFWGAVFYAILIYITLFYNLELTTAGGFFKLLSHNVITLFILYCSIYFTVLTMTYAI
                     LLMPLLVIKYFKGRQ"
     gene            2326..3141
                     /gene="cma"
                     /locus_tag="POB34_003829"
     CDS             2326..3141
                     /gene="cma"
                     /locus_tag="POB34_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II-degrading bacteriocin colicin M"
                     /protein_id="HDI5920692.1"
                     /translation="METLTVHAPSPSTNLPSYGNGAFSLSAPHVPGAGPLLVQVVYSF
                     FQSPNMCLQALTQLEDYIKKHGASNPLTLQIISTNIGYFCNADRNLVLHPGISVYDAY
                     HFSKPAPSQYDYRSMNMKQMSGNVTTPIVALAHYLWGNGAERSVNIANIGLKISPMKI
                     NQIKDIIKSGVVGTFPVSTKFTHATGDYNVITGAYLGNITLKTEGTLTISANGSWTYN
                     GVVRSYDDKYDFNASTHRGVIGESLTRLGAMFSGKEYQILLPGEIHIKESGKR"
     gene            complement(3191..3544)
                     /gene="cmi"
                     /locus_tag="POB34_003830"
     CDS             complement(3191..3544)
                     /gene="cmi"
                     /locus_tag="POB34_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104216667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M immunity protein"
                     /protein_id="HDI5920693.1"
                     /translation="MKVISMKFIFILTIIALAAVFFWSEDKGPACYQVSDEQARTFVK
                     NDYLQRMKRWDNDVQLLGTEIPKITWEKIERSSTDVEDEKTLLVPFKAEGPEGKRMYY
                     GMYHCEEGYVEYAND"
     gene            complement(3722..4513)
                     /locus_tag="POB34_003831"
     CDS             complement(3722..4513)
                     /locus_tag="POB34_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HDI5920694.1"
                     /translation="MSGPQLLSLPGSFLTPPATLPVAIDYPAALALRQMALVQDELPK
                     YLLAPEISALLHYVPDLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPFVQLATL
                     KQRTEKATRTAGRVPAGSQVHRLVPLSDTQYVSQLQMMVATLKIPLERRNKRTGRMEK
                     ARIWGITDRTVRTWLNEAVEAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQS
                     LMGHKSISSTEVYTKVFALDVAARHRVQFSIPESDAVTMLKNRHA"
     gene            complement(4510..5199)
                     /locus_tag="POB34_003832"
     CDS             complement(4510..5199)
                     /locus_tag="POB34_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920695.1"
                     /translation="MADWFIATEGVKVVKDSASLGPQIITAVSTIASAVGAVFLTHHF
                     TLRREERTAAAKLASERLYISTELVFLLERFAQRCAPAATATVEHDQDGRGEAEFLLP
                     EIDYSPVSGNWRSLPSGLMYRLSELSVLSYEAVVCVDNVFVEDTPYGGAGEYSLHKNA
                     AMLGVKALRLSRELRMLCGLPLHGLSDTLSPTRLVLLKARGKTLQKEYEMVKKSKKTE
                     QEIEDFIKGTS"
     gene            complement(5243..5593)
                     /locus_tag="POB34_003833"
     CDS             complement(5243..5593)
                     /locus_tag="POB34_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920696.1"
                     /translation="MFTAFNERNDFSYAFEKIRNAISAPGENNVYAATELGLGILLRK
                     YEQFRRELDAAGELGNWEYDLDTYNHCIAVLQRYFTGNPSGLTERDARIYSHYLQTEH
                     KGFVKLAEELAADR"
     gene            6126..7052
                     /locus_tag="POB34_003834"
     CDS             6126..7052
                     /locus_tag="POB34_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920697.1"
                     /translation="MSEKEHYMTNDGGESVTYTLRNIPADIDRVITDLATHVRKPKAT
                     FLREFLEDSFRDVIDSFALKNPLIASLDDELASYLGAEVMEKRYQSHYITRWNQEYQK
                     LLGISTEEELRRVVLTNTPFLHARAGQVLKGWKKIPRGISLTFSLFAEIAGRDRETID
                     RAWNRIFYSQLQEKKHRFYRDIDVIRALKKQHAVCGYSWTRDDITVRIYRPDDYGYGA
                     WRVLLVLDESVITQTWNIPFPELDGRRFTTDPGYDALISTAPDSWDKAFRFVDGICEL
                     HLYSNGVEEDQNPTPLPAVAEALIEAVVHVLL"
     gene            7102..7356
                     /locus_tag="POB34_003835"
     CDS             7102..7356
                     /locus_tag="POB34_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920698.1"
                     /translation="MKGRQSRYVTGGESFAEIARRPGGTVVMLCLNNGLEDALREASK
                     SLKSAFTRCGRRCRLAAGTAEGPFTGRRQGVATHLFVEVL"
     gene            7854..8198
                     /locus_tag="POB34_003836"
     CDS             7854..8198
                     /locus_tag="POB34_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HDI5920699.1"
                     /translation="MKDLFETEETVLFPFQTELHFVWTIYSIAKTLSVSACGHIGQKQ
                     YWSACLCILLLMAYVGLCAAVVWAVVPWLKAFFMAKYALSPFWFTPVKSLLLRGIGKM
                     AATICRKFTLLK"
     gene            complement(8372..9373)
                     /locus_tag="POB34_003837"
     CDS             complement(8372..9373)
                     /locus_tag="POB34_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HDI5920700.1"
                     /translation="MADPFNLQTDVVRQHTVPRFLLKHFSTPGKGKRQRLYAFDKAAG
                     RAYATTPDDATVRNTFYNLDNHPDRLSLEPLLGIYEHHAAPVIAALLAHRDIRRLTDD
                     ERYRLAVFVAVQRARTFGELERISGMISVLTDKMEAIGSTKEQAMETLGLSSGGDTKD
                     IFLRQLVQQVSHIDLLLKKDWYLLETRPERPFYVSDNPVVLKNSNDFGPYGNLGLAVR
                     GIQIYLPLSSTLMLAMYCPSIREQMVRQKQHLQHLLARAPHLIPRHIRPFERLEHIRR
                     YTDYLLMPLTPEHVTHYNSLQVEFAEQYVFCGEKDFSLVERMLADSERYRTGPRFTF"
BASE COUNT         2623 a         2309 c         2230 g         2612 t
ORIGIN      
        1 tcaaaatcgg tggagctgca tgacaaagtc atcgggcatt atctgaacat aaaacactat
       61 caataagttg gagtcattac ccatagtcat atattgcacc tccagccaca cccccagcag
      121 ctgctccaat tcctcctgca acaaattgtc cggatagtgt tcctatagcc atcgcaatat
      181 cacgccctga agcaccacca gaaacagaat ctaattcatt tagagtcaga gttctcatat
      241 gatctccttt ttatcttatc ggatattgaa taataattat caccaacaaa gtaacatatt
      301 gcagacatta atatagagaa gcaaaatgta tgcatggata aaaagtcctt tcctctaaaa
      361 acacaatcat atatagctaa tgcaatatat attgcggtgg catttattat aaatgcaaat
      421 aacaactcta attttgttct ttttctatcc attacttttt atcccattac tttctatccc
      481 attaccatac aaacactaac gataatgatt atcgttcaca tagtcaagag tgaaggggag
      541 ggggccctca ccccctataa ggggtccgct tggaaaacgg atttccccac gtcaagagaa
      601 ttgagttgaa cgagtggtta catccaagcc agctaactca ctgaacgtaa aggatgtacg
      661 aaatgctcct accgtccgat aaccatttgg cttacttccg tacattccct tgcgtagatg
      721 ttcagaattg tacgatttcg gacaccaaaa ccgctgttgt cccgccattc tggcgattcc
      781 tgaaagtcat ttgactaaaa aatagtctta acgtggttta ttttccgaat tccaagcggc
      841 ccgccctaca tttatacaat gtccggcagg gcataaaagg ggcgaaagaa aatacctggg
      901 cgaagtcgct gaacatcatt ctggcgattt caataaaggt acttgctcac gctctgtcat
      961 tttctgaaac tcttcatgct gcatttcgat tttccttcta agatcataac tcgcatacct
     1021 gttcttttta tgtatatcta tatctatttt tgtgacgagt tcaaaccaca atgttcgcac
     1081 tccccatgca gcgttatgaa acatttccgc aacggctata gagtctgtat taactgcttc
     1141 agtcatgtag tcgctaatac gttcagcggt aattttcgtt atttcatcgt aattcatttt
     1201 atctcctaca agattattta atggaatgca aaatatctgg cctgataata atgttccaga
     1261 attaatatct gcaggctatg ttaagttgtc cccacacaca atctacctgt tttctcgtgt
     1321 gtgccacagg agcaatacat aaatagtctg gccattaagt tcgtaaccag ctgctgtagc
     1381 tcttagtttg ttttctttga cattaggctc ggcccttata gcctttggtc ttccggccac
     1441 agttgttggt tttgttggcg tagttattgc aggtgctatt ggtgcattta tcgatgataa
     1501 atttgttgat gagttgaatc acaagatcat aaaataatta aaaggaaggc agtgttactg
     1561 cctcccttta aaatatttaa tgacaagcaa tggcatcagt aaaatcgcat atgtcatggt
     1621 taaaacagta aagtaaatgg agcagtataa aataaaaaga gtgatgacat tatgagataa
     1681 taatttaaaa aaaccacctg ctgttgttag ttccagatta tagaacagtg tgatataaat
     1741 cagaatggca taaaaaacgg ccccccagaa acaggattgc acaacaagtt ttcttgttac
     1801 tggctttatg atttctcttg tcttatatgt gcaaagaaaa acgagaatac ccataactgg
     1861 tgctgtcttg ttgtagtttg acataagtgc tgtcatgacg ggatcatgca atgatgtaat
     1921 agaagggaga aatgcaacct tattatataa gtagtacagc acttgagaat atggattatt
     1981 tatccgaagt attaatatag ccattaatgg aataataccg attatggaaa atgcatataa
     2041 tatttcgttt gctttcttgt tcttatcttt attgctggtc atatatatac ctttttgtat
     2101 ttttatatat agattatata tcagagttct caatatattt ccagtattta atggagtgta
     2161 cagatatata tcttatatat ggtagttcag gtgcttccct accattttta acggcatatc
     2221 cggcaaacaa tgttatgggg agcctctctc atggggttcg ttcgccggtg taagcgttag
     2281 ccagccgcga actctattat cttattaact tataaggagt tatgtatgga aaccttaact
     2341 gttcatgcac catcaccatc aactaactta ccaagttatg gcaatggtgc attttctctt
     2401 tcagcaccgc atgttcctgg tgctggacct cttttagtcc aggttgttta tagttttttc
     2461 cagagtccaa acatgtgtct tcaggcttta actcaacttg aggattacat caaaaaacat
     2521 ggggctagca accctctcac attgcagatc atatcgacaa atattggtta cttctgtaac
     2581 gccgaccgaa atctggttct tcaccctgga ataagcgttt atgacgctta ccacttctca
     2641 aaaccagcgc caagtcaata tgactatcgc tcaatgaata tgaaacaaat gagcggtaat
     2701 gtcactacac caattgtggc gcttgctcac tatttatggg gtaatggcgc tgaaaggagc
     2761 gttaatatcg ccaacattgg tcttaaaatt tcccctatga aaattaatca gataaaagac
     2821 attataaaat ctggtgtagt aggtacattc cctgtttcta caaagttcac acatgccact
     2881 ggtgattata atgttattac cggtgcatat cttggtaata tcacactgaa aacagaaggt
     2941 actttaacta tctctgccaa tggctcctgg acttacaatg gcgttgttcg ttcatatgat
     3001 gataaatacg attttaacgc cagcactcac cgtggcgtca tcggagagtc gctcacaagg
     3061 ctcggggcga tgttttctgg taaagagtac cagatactgc ttcctggtga aattcacatt
     3121 aaagaaagtg gtaagcgata atataagggc cgggattccg gcctttttat gcagccttta
     3181 taacagtgta ttagtcattc gcatattcaa catatccctc ttcacaatgg tacatgccat
     3241 aatacattct cttaccctcc ggaccttcag ctttaaatgg gacaagaagt gttttttcat
     3301 cttcaacatc tgttgaactt ctctcaatct tttcccatgt aattttcggg atttctgtac
     3361 caagaagttg tacatcgttg tcccagcgtt tcattctttg caggtaatca ttttttacaa
     3421 acgttctggc ctgttcatcg ctgacctgat agcatgccgg acctttatct tcagaccaga
     3481 aaaaaacagc agcaagagca ataatcgtta aaataaaaat aaatttcatg ctaattactt
     3541 tcataaaatc tccacaactt ctgtcgttca ttcgatataa aaaaacatta cgaatatatc
     3601 cgtacaatgt gagcatactg tgaagtggct ttaacaatta tgaaataata caagagatca
     3661 catattgcag tggtctcttc ttttatagaa taaatcacaa ttcataatta tgatttgtga
     3721 cttatgcgtg tctgtttttc agcattgtga cagcatcgga ctcaggaatc gaaaactgca
     3781 cccggtgccg tgcagccaca tccagcgcaa acaccttcgt gtacacctct gtcgaactaa
     3841 tcgacttgtg ccccatcagg ctctgcagca ccttcagcgg aataccagca tacagcatat
     3901 gcatcgcgta ggaatggcgg aaagtatgtg gggtaaccgg aacagagaac gtcacgccgt
     3961 cggccgcagc ggcctctaca gcttcattca gccaggtgcg aactgtgcgg tcggtgattc
     4021 cccagatgcg cgccttctcc attcttccgg ttcgtttgtt tctgcgctcc agtgggattt
     4081 tcagcgtggc caccatcatc tgcagttggc tgacgtattg tgtatcggac aggggaacca
     4141 ggcgatgcac ctgactgcca gcagggacac ggcctgccgt tctggtcgct ttttcagtcc
     4201 gttgtttgag cgtggccagc tgtacgaacg gatatggcgg cgccagagaa aaatctcccc
     4261 gcgtcagcgc cagcgcttcg tttatacgtg cgcccgtgtt ccacagggtg gccagcaaca
     4321 ttttgcggtg cagatccggt acatagtgga gcagggcgct gatctccggc gccaacagat
     4381 attttggtaa ttcgtcctgc acaagtgcca tctgacgtag tgccagcgca gccgggtaat
     4441 caatggcaac aggtaatgtt gccggaggcg ttaaaaaaga accaggcagg gaaagcaatt
     4501 gtgggccact catgatgtcc cctttatgaa atcctcaatc tcttgctcgg tctttttaga
     4561 cttctttacc atttcgtact ccttttggag tgttttacct ctggctttca acaataccaa
     4621 cctcgttggc gatagtgtat cggaaaggcc gtgcagaggg aggccacata acattctcag
     4681 ttctcgtgat aaccgcagtg cttttactcc caacatagct gcgtttttat gcaaactata
     4741 ttcacccgct ccaccataag gagtatcttc aacaaacaca ttatctacac acacgacagc
     4801 ttcgtagctg agaacagaga gttcgctgag cctatacatc aggccagaag gaagggagcg
     4861 ccagttacca gataccgggg aataatcgat ttccggtaat aagaactcag cttctcctct
     4921 gccatcctga tcgtgctcca cagttgctgt tgctgctgga gcgcaacgct gcgcaaatcg
     4981 ctcaagtaaa aaaaccagct ctgtactaat gtaaagacgc tcgctggcca gcttcgctgc
     5041 tgcagttctc tcctcacgtc tgagtgtgaa gtggtgagta aggaacaccg ctccgacagc
     5101 agatgcgatg gtcgatacag ctgtaattat ctgcggcccc agactggcgc tgtccttcac
     5161 caccttcacg ccttcggtcg caataaacca gtcagccatg tttattcctt tattgttgag
     5221 aaatcccgcc ttctgtatgt attcagcggt cggcagccag ttcttcagcc agcttcacaa
     5281 agcctttgtg ctcagtctgc aggtaatggc tgtaaatccg ggcatcacgt tctgtgagcc
     5341 cggatggatt gccggtgaag taacgttgca gcaccgcaat acagtgattg taggtatcga
     5401 gatcatattc ccagtttccc agctcaccgg ccgcatccag ctcccggcga aactgctcgt
     5461 atttacggag cagaatgcca agacctaatt ctgttgcagc ataaacatta ttttctcctg
     5521 gcgcggaaat cgcattacgg attttttcaa aagcataact gaaatcattt ctctcattga
     5581 atgcagtaaa catgtccttt cctcagttga atagttagcc gtgcaatttt accacacctc
     5641 aatcatcgcc agatgagtgc cactctgttg agaacagtga ctttcacaaa aaatgaattt
     5701 cacatactga aataataagt agccatatag aaacgcagtt aaattaccgg agagccgttt
     5761 ccgtcagatc cgactttccg gaatatcaca actttccgga agtgtaccac gctgagaaaa
     5821 taatcatatc gctgttttcc gcccctgcgc ggcgcatcac aggtcaggga gatgacacaa
     5881 catatagtgt catgagcagc tgaatgagat accacatgtt gtgctgacag gaggtggcgt
     5941 tgtgtggggg gagaggaaga tccgtaactg gagttttcgg atctcagaaa aaggggtgaa
     6001 cgggaaatat acagggtagc atacgaattt taaaaacata tgcttcgcag tatttaggta
     6061 ccctcaaatc caggatagtg gatgggcatt gcagtaatga atccacacat gaaaaggtaa
     6121 acgaaatgag tgaaaaagaa cattacatga caaacgacgg tggagagagc gtaacgtaca
     6181 cgctgcggaa cattcccgcc gacatcgacc gggtgatcac cgacctggca actcacgtcc
     6241 gtaagccaaa ggcgacgttt ctgcgtgagt tcctggaaga cagcttccgg gacgtcatcg
     6301 acagtttcgc cctgaaaaac ccgctcatcg cctctctcga tgacgagctg gccagttatc
     6361 tgggcgccga ggtcatggag aaacgctacc agagccatta catcacccgc tggaaccagg
     6421 aataccagaa gctgctcggg atctcgacgg aagaggagct tcgccgggtg gtgctgacca
     6481 acacgccctt cctgcacgca cgtgccggcc aggtgcttaa gggctggaaa aagatcccgc
     6541 gcgggatctc cctgaccttt tccctgtttg ccgagattgc cggccgggac agggaaacca
     6601 ttgaccgtgc ctggaacagg attttctact cgcagctgca ggagaaaaaa caccgctttt
     6661 accgggatat tgatgttatt cgtgccctga agaaacagca tgccgtctgc ggctacagct
     6721 ggaccaggga tgacatcacc gtgcgcattt accgcccgga tgactacggc tacggtgcct
     6781 ggcgggtgct gctggttctg gatgaaagtg tcattaccca gacgtggaac atcccgttcc
     6841 cggaacttga cggacgtcgc tttaccactg acccgggcta tgacgccctg atcagcaccg
     6901 ctcccgacag ctgggataag gccttccgtt ttgtggacgg gatttgtgaa cttcatcttt
     6961 acagcaatgg cgtggaagaa gatcagaatc cgactccgct gccagccgtc gctgaggcgc
     7021 tgattgaagc ggtggtgcac gttctactgt aacggacccg ggtgtacggg cacaaccaca
     7081 acggcatcat caggaggcat aatgaaaggt cgacagagcc gctatgttac cggtggggag
     7141 agtttcgcgg agatcgcccg tcgcccggga gggacagtgg tgatgctctg cctgaacaac
     7201 gggcttgagg atgcgctgcg ggaggcatcc aaatccctca agtcagcctt cacccgttgc
     7261 gggcgaagat gccggcttgc agcgggaacg gcggaaggac cgtttactgg tcgcaggcag
     7321 ggcgtggcga cccatctgtt cgtggaggta ctctgagggg cagggggtaa aaacttgaaa
     7381 aaagcattcg cctggtagcc ctgctgcggg tgaaaatgaa tgtacagaga gggaatggat
     7441 agcacttttt gctaaatgga tgtcagtatt actatgctat agttgcttta atgggaaatt
     7501 gcgccttgac aagggtaata ttttctatta gcattacttt catagtattg ttccgccccg
     7561 ctccaccccg acagatccgt ttatttcccg ccagactggt catcaccatt ccggccagga
     7621 tttttttgga tttttttccg gggagcaccc gggacgggct taaaatcggt atgacaaaca
     7681 ggaggatgcg aatgaacaca tcatagcaga gctgaaagag aaaacattct gtacggtatc
     7741 aacagcgttc acatcacgtg aggcgcagga tgccttttat ctgaaaaaag aggtcaagaa
     7801 gatgctcatc actaacacat caatgttaac aacaacctgc ccaggagggc cctatgaaag
     7861 atttgttcga aaccgaggaa acggttctat tcccattcca gactgaactc cattttgtct
     7921 ggacgattta ctccatagct aaaacccttt ccgttagtgc ttgtgggcac atcggacaga
     7981 agcaatactg gagcgcctgt ctctgtattt tgcttcttat ggcctatgtt ggtctctgtg
     8041 ctgcggtggt ctgggctgta gtgccctggc ttaaggcctt tttcatggcg aaatatgctc
     8101 tttcgccatt ctggttcacg ccagtaaaga gtttgttgct caggggtatc ggtaaaatgg
     8161 ctgccaccat ctgccgaaag tttactctat taaagtaaga gcagcaagct acactaacgg
     8221 atggtgaatg tcaacaggta ttcaccattt ccttacggcg gtggtccctg tggccactgc
     8281 cgctttcgcg tgggtgcaac acggcaaaac tcctctgtac aacaggggct cccgccgtca
     8341 ttttccggca caggtgaggc cggaattcgg actaaaacgt aaaccgcggg ccagtccggt
     8401 agcgttcact atcggccagc attctctcaa ccagagagaa atccttttca ccgcagaaca
     8461 cgtactgctc cgcgaactcc acctgcaggg aattgtaatg cgtgacatgc tccggggtaa
     8521 gcggcataag aagatagtcc gtatacctcc ggatatgttc cagtctttca aaaggccgaa
     8581 tgtgacgggg gataagatgc ggcgccctgg cgagcagatg ttgaagatgc tgcttttgac
     8641 gaaccatctg ttcacggatg gaggggcaat acatggccag catcagcgtc gatgacaggg
     8701 ggaggtaaat ctgtatcccg cgaacggcca gaccaagatt accgtacgga ccgaagtcat
     8761 tgctgttctt aagcactacg ggattgtctg atacgtaaaa cggacgttct ggcctggttt
     8821 ccagaaggta ccagtccttt ttcagcagga gatcaatatg tgacacctgc tggacgagct
     8881 gccgcagaaa aatatccttg gtatcgcccc cggacgacag gcccagggtt tccatggcct
     8941 gctctttagt cgagccgatg gcctccatct tatcggtaag caccgaaatc atgcctgaga
     9001 tacgctccag ttcaccgaag gttcgtgcgc gctgcacggc aacaaacacg gccagcctgt
     9061 agcgttcatc atctgtcagc ctgcgaatat ccctgtgcgc cagcagggct gcaattaccg
     9121 gtgccgcgtg gtgctcgtaa atccccagca gtggttcaag actcagacgg tccgggtgat
     9181 tatcgaggtt gtagaacgtg ttgcggacgg tcgcatcatc cggcgtggtg gcataggcgc
     9241 gcccggcagc cttgtcaaag gcgtataagc gctgccgctt accctttccc ggcgtactga
     9301 aatgcttcag taaaaaacgg ggaaccgtat gctgcctgac cacatcggtc tgcagattga
     9361 atggatcggc cataacttta tctccctgaa aagccacgca agcgcatcgg tgcatctgta
     9421 ttgacggcat gggcagctgg tttgcagctt agcattagaa aacagaaccg ttcgataatt
     9481 cctgtgttta acgccctgtc agtaccgatg gaatttaaat ctccggaacc gccggaaacc
     9541 ggatctgcgc ggcagaaaag acgcaaatga tatgataata aaaaatacac ataaaatcat
     9601 atcagatatg attttcatca ttgatggtaa aaccatatct gatattatca gaaatcagga
     9661 gcacacaaat catgcaggat atgattttta ccagtcagaa cattatcaga tggagcgtgc
     9721 aggatggatc aggaaatgac gttttcactc agctatgaac aactgacccg gttt
//
LOCUS       DAMZET010000158         9657 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.158, whole genome shotgun sequence.
ACCESSION   DAMZET010000158 DAMZET010000000
VERSION     DAMZET010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.158"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1392
                     /gene="zwf"
                     /locus_tag="POB34_003838"
     CDS             <1..1392
                     /gene="zwf"
                     /locus_tag="POB34_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDI5920701.1"
                     /translation="SLYQLEKAGQLNPDTRIIGVGRADWDKAAYTKVVREALETFMKE
                     TIDEGLWDTLSARLDFCNLDVNDTAAFSRLGAMLDQKNRITINYFAMPPSTFGAICKG
                     LGEAKLNAKPARVVMEKPLGTSLATSQEINDQVGEYFEECQVYRIDHYLGKETVLNLL
                     ALRFANSLFVNNWDNRTIDHVEITVAEEVGIEGRWGYFDKAGQMRDMIQNHLLQILCM
                     IAMSPPSDLSADSIRDEKVKVLKSLRRIDRSNVREKTVRGQYTAGFAQGKKVPGYLEE
                     EGANKSSNTETFVAIRVDIDNWRWAGVPFYLRTGKRLPTKCSEVVVYFKTPELNLFKE
                     SWQDLPQNKLTIRLQPDEGVDIQVLNKVPGLDHKHNLQITKLDLSYSETFNQTHLADA
                     YERLLLETMRGIQALFVRRDEVEEAWKWVDSITEAWAMDNDAPKPYQAGTWGPVASVA
                     MITRDGRSWNEFE"
     gene            1627..3438
                     /gene="edd"
                     /locus_tag="POB34_003839"
     CDS             1627..3438
                     /gene="edd"
                     /locus_tag="POB34_003839"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HDI5920702.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            3475..4116
                     /gene="kdgA"
                     /locus_tag="POB34_003840"
                     /gene_synonym="eda"
     CDS             3475..4116
                     /gene="kdgA"
                     /locus_tag="POB34_003840"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDI5920703.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(4172..5350)
                     /gene="purT"
                     /locus_tag="POB34_003841"
     CDS             complement(4172..5350)
                     /gene="purT"
                     /locus_tag="POB34_003841"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HDI5920704.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIKRAKHAAGQVKVQG
                     "
     gene            5484..5774
                     /gene="yebG"
                     /locus_tag="POB34_003842"
     CDS             5484..5774
                     /gene="yebG"
                     /locus_tag="POB34_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HDI5920705.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            5841..6197
                     /locus_tag="POB34_003843"
     CDS             5841..6197
                     /locus_tag="POB34_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HDI5920706.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            6524..7183
                     /locus_tag="POB34_003844"
     CDS             6524..7183
                     /locus_tag="POB34_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HDI5920707.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            7392..9452
                     /gene="ptrB"
                     /locus_tag="POB34_003845"
     CDS             7392..9452
                     /gene="ptrB"
                     /locus_tag="POB34_003845"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HDI5920708.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(9449..>9657)
                     /locus_tag="POB34_003846"
     CDS             complement(9449..>9657)
                     /locus_tag="POB34_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310581.1"
                     /note="3'-5' exonuclease activity on single or
                     double-strand DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HDI5920709.1"
                     /translation="TSGWTAEQMADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLR
                     WLFNNLDSMSPELRLTLKHYLENT"
BASE COUNT         2397 a         2398 c         2554 g         2308 t
ORIGIN      
        1 tccctgtatc aactggaaaa agccggtcag ctcaacccgg acacccggat tatcggcgta
       61 gggcgtgctg actgggataa agcggcatat accaaagttg tccgcgaggc gctcgaaact
      121 ttcatgaaag aaaccattga tgaaggttta tgggacaccc tgagtgcacg tctggatttt
      181 tgtaatctcg atgtcaatga cactgctgca ttcagccgtc tcggcgcgat gctggatcaa
      241 aaaaatcgta tcaccattaa ctactttgcc atgccgccca gcacttttgg cgcaatttgc
      301 aaagggcttg gcgaggctaa actgaatgct aaaccggcac gcgtagtcat ggagaaaccg
      361 ctggggacgt cgctggcgac ctcgcaggaa atcaatgatc aggttggcga atacttcgag
      421 gagtgccagg tttaccgtat cgaccactat cttggtaaag aaacggtgct gaacctgttg
      481 gcgctgcgtt ttgctaactc cctgtttgtg aataactggg acaatcgcac cattgatcat
      541 gttgagatta ccgtggcaga agaagtgggg atcgaagggc gctggggcta ttttgataaa
      601 gccggtcaga tgcgcgatat gatccaaaac cacctgctgc aaattctctg catgattgcg
      661 atgtctccgc cgtctgacct gagcgcagac agcatccgcg atgaaaaagt gaaagtactg
      721 aagtctctgc gccgcatcga ccgctccaac gtacgcgaaa aaaccgtacg cgggcaatat
      781 actgcgggct tcgcccaggg caaaaaagtg ccgggatatc tggaagaaga gggcgcgaac
      841 aagagcagca atacagaaac cttcgtagcg atccgcgtcg acattgataa ctggcgctgg
      901 gccggtgtgc cattctacct gcgtactggt aaacgtctgc cgaccaaatg ttctgaagtc
      961 gtggtctatt tcaaaacacc tgaactgaat ctgtttaaag aatcgtggca ggatctgccg
     1021 cagaataaac tgactatccg tctgcaacct gatgaaggcg tggatatcca ggtactgaat
     1081 aaagttcctg gccttgacca caaacataac ctgcaaatca ccaagctgga tctgagctat
     1141 tcagaaacct ttaatcagac gcatctggcg gatgcctatg aacgtttgct gctggaaacc
     1201 atgcgtggta ttcaggcact gtttgttcgt cgcgacgaag tggaagaagc ctggaaatgg
     1261 gtagactcca ttactgaggc gtgggcgatg gacaatgatg cgccgaaacc gtatcaggcc
     1321 ggaacctggg gacccgttgc ctcggtggcg atgattaccc gtgatggtcg ttcctggaat
     1381 gagtttgagt aatatctgcg cttatccttt atggttattt taccggtaac atgatcttgc
     1441 acagattgta gaacaatttt tacactttca ggcctcgtgc ggattcaccc acgaggcttt
     1501 ttttattaca ctgactgaaa cgtttttgcc ctatgagctc cggttacagg cgtttcagtc
     1561 ataaatcctc tgaatgaaac gcgttgtgaa tcatcctgct ctgacaactc aatttcagga
     1621 gcctttatga atccacaatt gttacgcgta acaaatcgaa tcattgaacg ttcgcgcgag
     1681 actcgctctg cttatctcgc ccggatagaa caagcgaaaa cttcgaccgt tcatcgttcg
     1741 cagttggcat gcggtaacct ggcacacggt ttcgctgcct gccagccaga agacaaagcc
     1801 tctttgaaaa gcatgttgcg taacaatatc gccatcatca cctcctataa cgacatgctc
     1861 tccgcgcacc agccttatga acactatcca gaaatcattc gtaaagccct gcatgaagcg
     1921 aatgcggttg gtcaggttgc gggcggtgtt ccggcgatgt gtgatggtgt cacccagggg
     1981 caggatggaa tggaattgtc gctgctaagc cgcgaagtga tagcgatgtc tgcggcggtc
     2041 gggctgtccc ataacatgtt tgatggtgct ctgttcctcg gtgtgtgcga caagattgtc
     2101 ccgggtctga cgatggcagc cctgtcgttt ggtcatttgc ctgcggtgtt tgtgccgtct
     2161 ggaccgatgg caagcggttt gccaaataaa gaaaaagtgc gtattcgcca gctttatgcc
     2221 gaaggtaaag tggaccgcat ggccttactg gagtcagaag ccgcgtctta ccatgcgccg
     2281 ggaacatgta ctttctacgg tactgccaac accaaccaga tggtggtgga atttatgggg
     2341 atgcagttgc caggctcttc ttttgttcat ccggattctc cgctgcgcga tgctttgacc
     2401 gctgccgctg cgcgtcaggt tacacgcatg accggtaatg gtaatgaatg gatgccgatc
     2461 ggtaagatga tcgatgagaa agtggtggtg aacggtatcg ttgcactgct ggcgaccggt
     2521 ggttccacta accacaccat gcacctggtg gcgatggcgc gcgcggccgg tattcagatt
     2581 aactgggatg acttctctga cctttctgat gttgtaccgc tgatggcacg tctctacccg
     2641 aacggtccgg ccgatattaa ccacttccag gcggcaggtg gcgtaccggt tctggtgcgt
     2701 gaactgctca aagcaggcct gctgcatgaa gatgtcaata cggtggcagg ctttggtctg
     2761 tctcgttata cccttgaacc atggctgaat aatggtgaac tggactggcg ggaaggggcg
     2821 gaaaaatcac tcgacagcaa tgtgatcgct tccttcgaac aacctttctc tcatcatggt
     2881 gggacaaaag tgttaagcgg taacctgggc cgtgcggtta tgaaaacctc tgccgtgccg
     2941 gttgagaacc aggtgattga agcgccagcg gttgtttttg aaagccagca tgacgttatg
     3001 ccggcctttg aagcgggttt gctggaccgc gattgtgtcg ttgttgtccg tcatcagggg
     3061 ccaaaagcga acggaatgcc agaattacat aaactcatgc cgccacttgg tgtattattg
     3121 gaccggtgtt tcaaaattgc gttagttacc gatggacgac tctccggcgc ttcaggtaaa
     3181 gtgccgtcag ctatccacgt aacaccagaa gcctacgatg gcgggctgct ggcaaaagtg
     3241 cgcgacgggg acatcattcg tgtgaatgga cagacaggcg aactgacgct gctggtagac
     3301 gaagcggaac tggctgctcg cgaaccgcac attcctgacc tgagcgcgtc acgcgtgggg
     3361 acaggacgtg aattattcag cgccttgcgt gaaaaactgt ccggtgccga acagggcgca
     3421 acctgtatca ctttttaaga cgacaaattt gtaatcaggc gagagaaaac tctgatgaaa
     3481 aactggaaaa caagtgcaga atcaatcctg accaccggcc cggttgtacc ggttatcgtg
     3541 gtaaaaaaac tggaacacgc agtgccgatg gcaaaagcgt tggttgctgg tggggtgcgc
     3601 gttctggaag tgactctgcg taccgagtgt gcagttgacg ctatccgtgc tatcgccaaa
     3661 gaagtgcctg aagcgattgt gggtgccggt acggtgctga atccacagca gctggcagaa
     3721 gtcaccgaag cgggtgcaca gttcgcaatt agcccgggtc tgaccgagcc gctgctgaaa
     3781 gctgctaccg aagggactat tcctctgatt ccggggatca gcactgtttc cgaactgatg
     3841 ctgggtatgg actacggttt gaaagagttc aaattcttcc cagctgaagc taacggcggc
     3901 gtgaaagccc tgcaggcgat cgcgggtccg ttctcccagg tccgtttctg cccgacaggt
     3961 ggtatttctc cggctaacta ccgtgactac ctggcgctga aaagcgtgct gtgcatcggt
     4021 ggttcctggc tggttccggc agatgcgctg gaagcgggcg attacgaccg tattactaag
     4081 ctggcgcgtg aagctgtaga aggcgctaag ctgtaaaagt caaaatgccc gatcgcggat
     4141 cgggcatttt tgtagcgttt ttttgccggg tttaaccctg tacttttacc tgtccggcgg
     4201 cgtgcttcgc gcgtttaatg gcgtcaacaa cactctcagc ggtcgccagc gcaacaccca
     4261 gacgacggct gccatcaatt tccggcttac caaataaacg aatctgcaaa tctgcgccta
     4321 cggcattctg cacattatca aacgtgacat tctgactggt cagttgtggc agaataacgg
     4381 cagaagctgc aggaccatac tgacggatcc cgccaacagg aagtccgagg aaggcacgta
     4441 catgcagggc aaactctgag agatcttgag aaattaacgt caccatcccg gtatcatgtg
     4501 gacgagggga gacctcactg aaaatcacct catcaccaca gacaaacagc tcgacaccaa
     4561 acagaccata accgcccagt gccagcacca ctttacgggc aatctcctgc gcacgttcaa
     4621 gggcaagcgg gctcatttgc tgtggttgcc aggattcacg gtagtcgcca tcttcctggc
     4681 gatgacctac tggtgcacag aaatggacgc catccaccgc gctgacggtt agcagggtaa
     4741 tttcgaagtc aaacttaacg acgccttcaa caattacgcg gcccgctccg gcgcgaccgc
     4801 cttgctgagc gtattcccat gcctgagcaa gttgctctgc agaacgaata aacgtctgcc
     4861 ccttgccgga agagctcatc accggtttta caatgcaggg atagccaatg gcggcaaccg
     4921 cctcgcggaa aaggctttcg ctatcggcaa aacgataagt ggaagtgggc agctgcagct
     4981 cttctgccgc caggcgacgg ataccctcgc gattcatcgt taatttcgtt gcgcgagcgc
     5041 aggggacaac attcagtcct tcctcttcaa gttggatcag catatcggtg gcaatagctt
     5101 cgatctccgg cacgatataa tgtggttttt ccagttcaac cacacggcgt aatgcatcac
     5161 catcaagcat attaatgaca tgggagcgat gcgcgacatg catggctggt gcgtcggcat
     5221 agcgatcgac ggcaatcacc tctacgccga gacgctgaca ctcgattgcc acttctttac
     5281 ccagttcacc ggagcctaat aacatcacgc gagttgctgc cggacgcagc gcagtgccta
     5341 ataacgtcat aacgaatatc ccctgattaa ttccgcgcgc agtataaacg aaaacgtttg
     5401 cgtgtgtctt tattgctgat gttgatttct caaccgaaaa gaaatatact gtataaaatc
     5461 acagttatta tgagaggctg caaatggcgg ttgaagttaa atacgtagtc attcgtgagg
     5521 gcgaagagaa aatgtcgttt accagcaaaa aggaagccga tgcttatgat aaaatgcttg
     5581 atacggcgga tctgcttgac acctggctga caaattctcc agtgcaaatg gaagacgagc
     5641 aacgtgaagc cctttcgcta tggctggcag aacaaaaaga tgtgctgagc accattctga
     5701 aaaccggcaa attaccgtcc ccacaggtcg tcggtgctga atccgaagag gaagatgcct
     5761 cacacgcggc ctgaagcaac ttgcgctcct gacaatttgt accatgcttt ctcctgaatc
     5821 attgtgctat ggagaaaaac atgaaaaaaa gaggggcgtt tttagggctg ttgttggttt
     5881 ctgcctgcgc atcagttttc gctgccaata atgaaaccag caagtcggtc actttcccaa
     5941 agtgtgaagg tctggatgct gccggaattg ccgcgagcgt aaaacgtgat tatcaacaaa
     6001 atcgcgtggc gcgctgggca gatgatcaaa aaattgtcgg tcaggccgat cccgtggctt
     6061 gggtcagttt gcaggacatt cagggtaaag atgataaatg gtcagtaccg ctaaccgtgc
     6121 gtggtaaaag tgccgatatt cattaccagg tcagcgtgga ctgcaaagcg ggaatggcgg
     6181 aatatcagcg gcgttaatgg cttctgataa ttcgattgac cgttggcgga tgcggcgcga
     6241 gtgccttgtc cgccccagaa cgcaccacaa tcgtaggcct gagaagacgc aacagcgtcg
     6301 catcgggcaa tcagcatcaa acaatgccac tcaaaacgac aaactacttt cacctaaaat
     6361 acccttatca cctgatatac agtaaatacc ttacctggcg cgaatttctt tgaataacgg
     6421 cctaccgccc cgcttatttg cgcatctttt gtctcgatat acattttctg acatcctcat
     6481 ccgctatgct acaggcacta aaaatggtta ttgaggagca aatatggcta actggttaaa
     6541 tcaactgcaa tccctgcttg ggcaaagcag ttcttctacc tcctcgtctg cggatcaggg
     6601 attgggcaaa ctgttagtgc caggcgcatt aggcggactg gctgggctgc tggtcgcaaa
     6661 taaatcagca cgtaaacttc ttacgaaata tggcaccaac gcgttactgg ttggcggcgg
     6721 agcggtagcg ggtacggtgc tgtggaataa atacaaagat aaaattcgcg cggcgcatca
     6781 ggacgaaccg cagtttggcg cgcaaagtac gccgctggat gagcgtacag aacgtttgat
     6841 ccttgcgctg gtctttgccg ctaaaagtga tggtcatatt gatgccaaag aacgtgcggc
     6901 aatcgaccag caattgcgtg aagccggcgt ggaagagcag gggcgtgtac tcattgagca
     6961 ggcaatcgaa caaccgctgg atccacaacg cctggctacc ggggtccgca atgaagaaga
     7021 ggcgctggaa atctatttcc tgagttgcgc ggctattgat attgaccatt ttatggaacg
     7081 cagttacctg aatgcactgg gtgacgcgct gaaaatcccc caggatgttc gtgacggcat
     7141 tgaacgcgat ctcgaacagc aaaaacgcac tttggcggaa taattccgct ctctggatag
     7201 cctgaagttg actatttcgg gctatccaga atcgcacagc catttgaaca ggctgatttt
     7261 ctctgtctgc ataacattcg atatccgcgc cattacctgt catcatctaa gcaatgactc
     7321 ccctgtttcg cttgcatccc cggtgagttt tgccaccctt ataagatgtt tcaaccagaa
     7381 agaacaataa catgctacca aaagccgccc gcattcccca cgccatgacg cttcatggcg
     7441 atacgcgcat cgataattac tactggctgc gggacgatac gcgttctcag ccagaagtcc
     7501 tggactacct gcaacaagaa aatagttacg gtcatcgggt gatggcctca caacaagcct
     7561 tgcaggatcg catcttaaag gaaatcatcg accgcattcc gcaacgagaa gtttctgcgc
     7621 cctacatcaa aaatggctac cgctatcggc atatttatga accaggctgt gaatatgcta
     7681 tctaccagcg tcaatcggca ttcagtgaag agtgggatga gtgggaaaca ttgctcgatg
     7741 ccaataagcg cgcagctcat agtgagtttt attcgatggg cggaatggcg attacgcccg
     7801 ataacaccat tatggcgctg gcagaagatt ttctttcccg acgccagtac ggcattcgtt
     7861 ttcgtaatct ggaaactggt aactggtacc cggaactgct ggataacgtt gaacccagct
     7921 ttgtctgggc aaatgactcc tggactttct actatgttcg caagcatccg gtgacgctgc
     7981 tgccttatca ggtctggcgt cacgccatcg gtacgccagc atcgcaagat aaactgatct
     8041 acgaagaaaa agacgatacc tattacgtca gcctgcataa aacgacctcg aagcactatg
     8101 tagtcattca tttggccagc gccaccacca gtgaagttcg cctgctggac gcggaaatgg
     8161 ccgatgccga gccgtttgtt tttctgccgc gccgcaaaga tcacgaatac agccttgatc
     8221 actaccagca tcgtttttat ctgcgttcca accgccacgg caaaaacttt ggcttatacc
     8281 gtacccgtat gcgtgatgag caacagtggg aagagttaat tccgccacgc gaaaacatca
     8341 tgctggaagg gtttacgctg tttaccgact ggctggtggt tgaagagcgt cagcgcgggt
     8401 taaccagttt gcgccaaatt aaccgcaaga cccgggaagt cattggtatt gcctttgatg
     8461 atccagccta tgtgacctgg attgcctaca atccagaacc tgaaaccgcg cgattgcgtt
     8521 atggttattc ttccatgacc acaccagaca ctttgtttga actggatatg gataccggtg
     8581 agcgtcgtgt attaaaacaa acagaagtgc ccggttttga tgcggcgaat taccgcagtg
     8641 aacacctgtg gatagtcgcc cgtgatggcg tcgaagttcc ggtttcgttg gtctaccatc
     8701 gcaaacattt tcgcaaagga cacaacccgt tgctggtgta tggctatggt tcttacggcg
     8761 caagtattga tgccgatttc agttttagcc gcttgagttt gttagatcgt ggctttgtct
     8821 acgccattgt ccatgttcgc ggcggtggtg agctggggca acaatggtac gaagacggaa
     8881 aatttctgaa gaagaaaaat acgtttaatg attatcttga tgcctgcgat gcattgttaa
     8941 aactgggcta tggctctcct tcgctttgtt atgcgatggg cgggagtgcg gggggcatgt
     9001 tgatgggcgt tgcaattaat caacgcccgg aattattcca cggcgttatc gcccaggtac
     9061 cgtttgttga tgttgtaaca acgatgcttg atgaatcaat tcctcttacc actggtgagt
     9121 ttgaagagtg ggggaatccg caggatccgc aatattacga gtacatgaaa agctacagcc
     9181 catatgacaa cgtcaccgca caggcttatc cgcatttact ggtaacgacc ggtttgcacg
     9241 attctcaggt gcaatattgg gaaccggcaa aatgggtcgc taaattgcgc gagctgaaaa
     9301 ccgatgacca tcttttattg ctctgtaccg acatggactc aggccatggc ggtaaatctg
     9361 gtcgctttaa atcgtacgaa ggcgtagcga tggaatatgc ttttctggtc gcgctggcgc
     9421 agggaacatt acccgctacg cctgcggact aagtattttc cagataatgt ttcagtgtta
     9481 aacgcagctc cgggctcatg ctgtccaggt tattaaataa ccagcgcaga tagcccggat
     9541 cgcgttcggc aacgtcggaa accgctttgc cacggtattt gccaaaggtg aaggtcgtca
     9601 tcaacgacgg acgtccggtg atatcggcca tctgttctgc cgtccagccg gaggtgt
//
LOCUS       DAMZET010000159         9641 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.159, whole genome shotgun sequence.
ACCESSION   DAMZET010000159 DAMZET010000000
VERSION     DAMZET010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.159"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..347)
                     /locus_tag="POB34_003847"
     CDS             complement(<1..347)
                     /locus_tag="POB34_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308998.1"
                     /note="in Escherichia coli the CydCD ABC transporter
                     exports cysteine and glutathione into the periplasm in
                     order to maintain redox balance; important for cytochrome
                     bd and c; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HDI5920710.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYR"
     gene            complement(348..2114)
                     /gene="cydD"
                     /locus_tag="POB34_003848"
     CDS             complement(348..2114)
                     /gene="cydD"
                     /locus_tag="POB34_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HDI5920711.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(2237..3202)
                     /gene="trxB"
                     /locus_tag="POB34_003849"
     CDS             complement(2237..3202)
                     /gene="trxB"
                     /locus_tag="POB34_003849"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDI5920712.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            3746..4240
                     /gene="lrp"
                     /locus_tag="POB34_003850"
     CDS             3746..4240
                     /gene="lrp"
                     /locus_tag="POB34_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HDI5920713.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            4375..8481
                     /gene="ftsK"
                     /locus_tag="POB34_003851"
     CDS             4375..8481
                     /gene="ftsK"
                     /locus_tag="POB34_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDI5920714.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQQQYQQPQQPV
                     APQPQYQQPQQQVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            8636..9247
                     /gene="lolA"
                     /locus_tag="POB34_003852"
     CDS             8636..9247
                     /gene="lolA"
                     /locus_tag="POB34_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HDI5920715.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            9258..>9641
                     /gene="rarA"
                     /locus_tag="POB34_003853"
                     /gene_synonym="mgsA"
     CDS             9258..>9641
                     /gene="rarA"
                     /locus_tag="POB34_003853"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HDI5920716.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERIYAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLP"
BASE COUNT         2418 a         2499 c         2549 g         2175 t
ORIGIN      
        1 tgacgatagc gcgccagtcc ggcaggggag aggggcagca atttgctgaa ggtgtaaatg
       61 cgcagatgct gcaacacgcg gaaagtcgcg tcgtgactta ccagacgttc aaaataacgc
      121 ccggcagtac gggtgattgc tgcgccacgc acgcccgcag cgggtagcat atagttgaag
      181 ctgtacagtc cggcaacccc cgcaaccgct gaggccgaga ggaaccagcc ggaaagtgtc
      241 aacagaccga tactggcgag cagcgtgaca attgccagca cgataccaag acttaacatc
      301 catttatgac gtttatacag tgccagatag ggtagcaaag cgcgcattta aatctcctcc
      361 tgacgatggg ccagtaatgt ggcgaatggg ccaccagcca cacttaattc cgcgtaacgt
      421 ccttgctcaa taatccggcc atcctgcata acccaaatga catcccagtc agcaagatct
      481 tctaactggt gggtgaccat taacgttgtc tggcgcagag aggcggcatt cagcgcctcc
      541 attacgcgct gttcactgtg agcatcaagg ctggcagcgg gttcatccaa cagtaatagc
      601 gaacagggat ttagtaacgc acgggccacc gccacgcgct gcgcctgccc cacggaaagg
      661 cgggcagcct ggtcgccaac aggtgtatca acgccttgtg gcaggagcgg tagaaactcg
      721 ctgacccagg cgttatccag cgctgcttgt aattcttgtt cgctggcatc aggtcgcgcc
      781 agtagtacgt tatcccgcaa tgttgctgcc ggtaattgtg ggttttgccc aacccaggag
      841 agatgtttac gccatgattc tggtgataaa tcgcgtaatt ctatcccgtt gattcgtaat
      901 gatccctgat atgagagaaa accagaaagc gcgttcagca gtgagctttt acctgaaccg
      961 ctgcgaccaa ccaacactgc acgttggcct gctggcaaag taaagttcag cggtccggcc
     1021 agcgttttac cttccggcga cgtgataaac agatcctcgg cctcaatggt caccggatcg
     1081 gtcgatgcta attccacctc gccacgctgc ggatgggcga gcggggtttc cataaacgtt
     1141 ttcagactgt cagctgcacc aacagcctgg gctttagcat gataaaacgt accgagatcg
     1201 cgcaatggct ggaaaaactc tggcgcaagg atcagggcca gaaaacccgc agccagcgtc
     1261 acaccggtat cgtagtgacc aaaatccagc tcgccgagat aggaaaaacc aaagtagacc
     1321 gccaccagag caattgacag cgaggtaaaa aattcgagaa tgccggagga taaaaacgcc
     1381 agccgtagca cttccattgt ccgttggcgg aaatcttccg aagcagaacg aatactttca
     1441 atttcagctt caccacgacc aaaaatacgc aatgtttcca tgccgcgcag gcgatcgagg
     1501 aaatgcccac ttaagcgagc aagagcgaga aagttacgcc ggttagcatc ggcagccccc
     1561 atcccaacca gcgccataaa caacggaatt aacggtgctg tgcccagcag aatgagcgcc
     1621 gcagcccagt tagaggggaa gatcgccacc acaatcagca acggcaccga cactgccagc
     1681 gccatttgcg gcaggtagcg tgcatagtaa tcatgcatat cgtcaatttg ctcgagtacc
     1741 aacgtcgccc agctccccgc aggtttaccc tgaatccacg ctggccctgc ttgttgcaga
     1801 cggtcgagaa cctgacggcg gatggcaaag cggatatgct gcccggcgtg ataacccacc
     1861 cgttcgcgta accagaccac ccatgcgcgc agtacaaagg tcagaaccag taacgtaaag
     1921 ggaagcagca gggcttcacg gggaatattc tccataatca tatgttgcag aattcgcgcc
     1981 atgaaccagg cctgggcaat gatcaatatg ccgcttacaa agcccagcag acgagaaata
     2041 ttcagccaac gttgggagat gacgctttgc tgttttaacc agcgggttaa ctctttttga
     2101 cgggatttat tcattgcacg cttagcaggt gagttatcag aattatttgc agagcaatgt
     2161 tacaacgggg aaaaaataaa ggcgacccat agtcgcatgg tgtcgccttc tttacttttg
     2221 ttactgattt gtaaaattat tttgcgtcag ctaaaccatc gaggtagcgt tccgcatcaa
     2281 gtgctgccat gcagcctgta ccggccgaag taatggcctg acgataaata tgatccatca
     2341 cgtcacccgc ggcaaagacg ccaggaatgc tggtctgggt ggcattacca tgaatacccg
     2401 actgcacttt gatgtagccg ttttccagtt ccagctgccc ttcgaaaatg gcagtattcg
     2461 ggctgtgacc gatagcaaca aacagaccag caacgtcgag tgactcgatg ttatcgctgt
     2521 tttgcgtatc gcgcagacga acgccagtga cacccatttg atcgccggtc acttcttcca
     2581 gcgtacggtt ggtgtgcaga atgatgttgc cgttctccac tttatccatc agacgcttaa
     2641 taaggatttt ttccgcgcgg aaaccgtcac ggcggtgaat aagatgcact tccgaagcga
     2701 tgttagacag atacagcgcc tcttcaaccg cggtattgcc gccgccgatg accgcaactt
     2761 tctggttgcg atagaagaaa ccgtcgcagg ttgcacaagc agaaacccca cggcctttaa
     2821 aggcttcttc agagggcagg ccgagatagc gtgcagaagc tccggtggca ataatcagcg
     2881 cgtcgcaagt gtattcgccg ttatcgccat tcagacggaa cggacggttt tgcagatcca
     2941 ccttgttgat atgatcaaaa atgatctcag tttcaaactt ggtggcatgt tcgtgcatgc
     3001 gctccattaa taacggaccg gtcagatcgt ttggatcgcc aggccagttt tccacttccg
     3061 tggtggtggt cagttggccg cctttttcca tgccggtaat cagcacaggt tgcaggttgg
     3121 cgcgcgccgc gtagacagca gcggtgtatc ccgccgggcc tgaacccagg ataagcagtt
     3181 tactgtgttt ggtcgtgccc atgagatccc catagttgtt ggcagacaat gggcaggatt
     3241 gtagggaatt tacagacgta aaaaaagagt atgacgattt tgttaacaat ttgtgcaatc
     3301 ggcagcatcg ataagcaggt caaattctcc cgtcattatc acctctgcta cttaaatttc
     3361 ccgctttata agccgattaa atgatgaata aacgcccctg ttaatgaata tctggcatgt
     3421 tgtactaaaa atcgatgttt tgctttgaca atcccctggt gttttgcgaa aacattcgag
     3481 gaagaaaaaa acagtattct tatatgcgca taaccatgca tgtaaatacc atgtttaccg
     3541 tgctagtgaa atctacgtat ggcgtggaca gacgccattc gtgatgtcga tagctgccac
     3601 aaggcaacgg tcttctcacc gtagacccag gcattgcgcg ccgtgaatct tcatgatttc
     3661 ggtctatcgt gacgggtagc gactctgaac agtgatgttt cagggtcaga caggagtagg
     3721 gaaggaatac agagagacaa taataatggt agatagcaag aagcgccctg gcaaagatct
     3781 cgaccgtatc gatcgtaaca ttcttaatga gttgcaaaag gatgggcgta tttctaacgt
     3841 cgagctttct aaacgtgtgg gactttcccc aacgccgtgc cttgagcgtg tgcgtcggct
     3901 ggaaagacaa gggtttattc agggctatac ggcgctgctg aacccccatt atctggatgc
     3961 atcacttctg gtattcgttg agattactct gaatcgtggc gcaccggatg tgtttgaaca
     4021 attcaatacc gctgtacaaa aacttgaaga aattcaggag tgtcatttag tatctggtga
     4081 tttcgactac ctgttgaaaa cacgcgtgcc ggatatgtca gcttaccgta agttgctggg
     4141 ggaaaccctg ctgcgtctgc ctggcgtcaa tgacacacgg acatacgttg ttatggaaga
     4201 agtcaagcag agtaatcgtc tggttattaa gacgcgctaa cacggaacag gtgcaaaatc
     4261 ggcgtatttt gattacactc ctgttaatcc atacagcaac agtactgggg taacctggta
     4321 ctgttgtccg ttttagcatc gggcaggaaa agcctgtaac ctggagagcc tttcttgagc
     4381 caggaataca ctgaagacaa agaagtcaca ttgacaaagt taagtagcgg ccgccgcctt
     4441 ctggaggcgt tgctgatcct tattgtcctg tttgccgtct ggttgatggc tgccttacta
     4501 agctttaacc cttcggaccc cagctggtcg caaacggcct ggcatgaacc tatccataat
     4561 ttaggtggga tgcccggtgc gtggttggca gatacgctgt tctttatttt tggcgtgatg
     4621 gcttacacca ttcccgtcat tattgtcggc ggttgttggt ttgcctggcg tcatcagtcc
     4681 agcgacgaat acattgatta ttttgccgtt tcgctacgca tcattggcgt tttggcgctc
     4741 atccttacct cctgtggtct ggcggcaatc aacgctgacg atatctggta ttttgcctcc
     4801 ggtggcgtca ttggcagctt actaagtact acgctacaac cactgctaca cagtagcggg
     4861 ggaactattg cgctgctctg cgtttgggct gcgggcctga cgctgttcac cggttggtca
     4921 tgggtgacca ttgctgaaaa actcggcggc tggattttaa acattctcac cttcgccagt
     4981 aatcgtaccc gtcgcgatga tacctgggtc gatgaagatg aatatgaaga tgacgaagag
     5041 tatgaagatg aaaaccacgg caaacagcat gaatcacgcc gtgcccgtat tcttcgcggc
     5101 gcgctagcgc gtcgtaaacg gttggcggaa aaattcatta atccgatggg gcggcaaaca
     5161 gacgctgcgt tgttctccgg taagcggatg gatgatgacg aagagattac ctacactgca
     5221 cgcggtgtgg ctgccgaccc ggacgacgtc ctattttcgg gcaatcgtgc aacgcagcca
     5281 gaatatgacg aatacgatcc attattaaac ggtgcgccaa ttaccgaacc tgtcgctgta
     5341 gcagctgctg ctaccacggc gacacaaagc tgggctgcgc cggttgaacc tgtgactcag
     5401 acgccgcctg ttgcctctgt tgatgttcca ccctcgcaac ctacagtagc ctggcagcct
     5461 gtaccgggtc cacaaacggg agagccggtt attgctcctg caccggaagg ttacccacag
     5521 cagtcacaat atgcgcagcc tgcagtgcaa tataatgagc cgctgcaaca accagtacag
     5581 ccgcagcagc cgtattatgc acctgcagct gaacaacctg cgcaacaacc ttactacgct
     5641 cctgcggcgg aacaacctgt tcagcaaccg tattacgcaa ctgcgccgga acagcctgca
     5701 caacagccgt attacgcccc tgcgccagaa caaccggtgg caggtaacgc ctggcaagcc
     5761 gaagagcagc aatccacttt tgctccacag tctacatacc agactgagca aacttatcag
     5821 caaccagccg ctcaggagcc gttgtaccaa cagccgcaat ccgttgaaca gcagcctgtt
     5881 gtggagcctg aacccgttgt agaagagaca aaacccgcgc gtccgccgct ttactatttt
     5941 gaagaagtgg aagagaagcg agcccgtgaa cgtgaacaac ttgcggcctg gtatcaaccg
     6001 attccagaac cggttaaaga accagaaccg atcaaatctt cgttgaaagc gccttctgtt
     6061 gcagcagtac ctccagtaga agccgctgcc gctgtttctc cgctggcatc tggcgtgaaa
     6121 aaagcgacac tggcgacggg ggctgccgca accgttgccg cgccagtctt cagtctggca
     6181 aatagcggtg gaccgcgtcc gcaagtcaaa gaggggattg gcccgcagtt gccacgaccg
     6241 aaacgtatcc gcgtgccaac tcgtcgtgaa ctggcgtctt acggtattaa gctgccctca
     6301 cagcgtgcgg cggaagagaa agcccgtgaa gcccagcgca atcagtatga ttctggcgat
     6361 cagtataacg atgatgaaat cgatgcgatg cagcaggatg aactggcacg tcagttcgcc
     6421 cagacacagc agcaacgcta tggcgaacag tatcaacatg atgtgcccgt aaacgcagaa
     6481 gatgcagatg ctgcggcaga ggctgaactg gctcgtcagt ttgcgcaaac tcaacaacaa
     6541 cgttattccg gcgaacaacc ggctggggcg aatccgttct cgctggatga ttttgaattt
     6601 tcgccaatga aagcgttgct ggatgatggt ccacacgaac cgttgtttac gccaattgtt
     6661 gaacctgtac agcagccgca acaaccggtt gcaccgcagc agcagtatca gcagccgcaa
     6721 caaccagttc cgccacagca gcaatatcag cagccgcaac agccggttgc gccgcagccg
     6781 cagtatcagc agccacaaca gcaggttgcg ccgcagccac aatatcagca gccgcaacaa
     6841 ccggttgcgc cacagccgca atatcagcag ccgcaacaac cagttgcgcc gcagccgcag
     6901 tatcaacagc cgcaacaacc ggttgcaccg cagcagcagg ataccctgct tcatccgctg
     6961 ttgatgcgta atggcgacag ccgtccgttg cataaaccga cgacgccgct gccttctctg
     7021 gatttgctga caccgccgcc gagcgaagtg gagccggtag atacctttgc gcttgaacaa
     7081 atggctcgcc tggtggaagc gcgtctggct gatttccgta ttaaagccga tgtcgtcaat
     7141 tactctccgg ggccggttat cactcgcttt gaacttaacc tggctcctgg cgtaaaagcg
     7201 gcgcgcattt ctaacttgtc acgggacctt gcccgttcac tttcgacggt ggcggtgcgt
     7261 gtcgttgaag ttattcctgg caaaccctat gtaggtctgg agttaccaaa taaaaaacga
     7321 caaaccgttt atctgcgcga agttttggat aacgccaaat tccgcgataa tccgtcgcca
     7381 ttaaccgtgg tgctgggtaa agatatcgcc ggtgagccgg tggttgccga tctggcgaaa
     7441 atgccgcact tgttggttgc ggggactacc ggttccggta aatctgtcgg tgtgaacgcg
     7501 atgatcctga gcatgcttta taaagcacag ccagaagatg tgcgtttcat catgatcgac
     7561 ccgaaaatgc tggagctttc ggtttatgaa ggcattccgc atctgttaac ggaagtcgtt
     7621 actgatatga aagatgccgc caacgcgctg cgctggtgtg ttaacgagat ggagcgtcgt
     7681 tataaactga tgtctgcgct gggtgtgcgt aatctggcgg gttataacga aaaaattgct
     7741 gaagccgatc gcatgatgcg tccgattcca gacccgtact ggaagccggg tgacagtatg
     7801 gatgcccagc atccggtgct gaaaaaagaa ccatacattg tggtgttggt tgacgaattt
     7861 gccgacctga tgatgacggt aggtaaaaaa gtggaagagc tgatagcacg tctggcgcaa
     7921 aaagcccgtg ccgcgggtat ccacctcgta ctggcaactc agcgtccatc ggttgatgtt
     7981 attactggtc tgattaaagc gaatattccg acccgtatcg cctttaccgt atccagtaag
     8041 attgactcac gtaccattct tgatcaggct ggcgcggaat cactgctggg gatgggggat
     8101 atgctctact ctgggccgaa ctccacgttg ccggtacgtg tccatggtgc ttttgttcgc
     8161 gatcaggaag ttcatgccgt ggtgcaggac tggaaagcgc gtggtcgccc acagtatgtt
     8221 gatggcatca cttccgacag tgaaagcgaa ggtggcgcgg gcggtttcga tggcgctgaa
     8281 gaactggatc cgttgttcga tcaggcggtg cagtttgtca ctgaaaaacg caaagcgtca
     8341 atttctggcg tacagcgtca gttccgcatt ggttataacc gtgcagcgcg tattatcgaa
     8401 cagatggaag cgcaggggat tgtcagcgaa caggggcaca acggtaatcg tgaagtgctg
     8461 gccccaccgc cgtttgacta attaatgcat tgccggataa ggcgcggtag cgtcgcatcc
     8521 ggcactctat caactgaaaa ttcagtattt tcttctttcc tcaagctgat tattagcctg
     8581 gaatagagag tagagggaac tcccgatcgg gagtgacgta atttgaggaa taatgatgaa
     8641 aaaaattgcc atcacctgtg cattactctc aagcttagta gcaagcagcg tttgggctga
     8701 tgccgcaagc gatctgaaaa gccgcctgga taaagtcagc agcttccacg ccagcttcac
     8761 acaaaaagtg actgacggta gcggcgcggc ggtgcaggaa ggtcagggcg atctgtgggt
     8821 gaaacgtcca aacttattca actggcatat gacacaacct gatgaaagca ttctggtttc
     8881 tgacggtaaa acactgtggt tctataaccc gttcgttgag caagctacgg caacctggct
     8941 gaaagatgcc accggtaata cgccgtttat gctgattgcc cgcaaccagt ccagcgactg
     9001 gcagcagtac aatatcaaac agaatggcga tgactttgtc ctgacgccga aagccagcaa
     9061 tggcaatctg aagcagttca ccattaacgt gggacgtgat ggcacaatcc atcagtttag
     9121 cgcggtggag caggacgatc agcgcagcag ttatcaactg aaatcccagc aaaatggggc
     9181 tgtggatgca gcgaaattta ccttcacccc gccgcaaggc gtcacggtag atgatcaacg
     9241 taagtagagg cacctgagtg agcaatctgt cgctcgattt ttcggataat acttttcaac
     9301 ctctggccgc gcgtatgcgg ccagaaaatt tagcacagta tatcggccag caacatttgc
     9361 tggctgcggg gaagccgttg ccgcgcgcta tcgaagccgg gcatttacat tctatgatcc
     9421 tctgggggcc accgggtacc ggcaaaacaa ccctcgctga agtgattgcc cgctatgcga
     9481 acgctgatgt ggaacgtatt tatgccgtca cctctggcgt gaaagaaatt cgcgaggcga
     9541 tcgagcgcgc ccggcaaaat cgcaatgcag gtcgccgcac tattcttttt gttgatgaag
     9601 ttcaccgttt caacaaaagc cagcaggatg catttctgcc a
//
LOCUS       DAMZET010000160         9550 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.160, whole genome shotgun sequence.
ACCESSION   DAMZET010000160 DAMZET010000000
VERSION     DAMZET010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.160"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(109..174)
                     /locus_tag="POB34_003854"
     CDS             complement(109..174)
                     /locus_tag="POB34_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920717.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            409..1296
                     /gene="rhtA"
                     /locus_tag="POB34_003855"
     CDS             409..1296
                     /gene="rhtA"
                     /locus_tag="POB34_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HDI5920718.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            1595..2098
                     /gene="dps"
                     /locus_tag="POB34_003856"
                     /gene_synonym="pexB"
     CDS             1595..2098
                     /gene="dps"
                     /locus_tag="POB34_003856"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HDI5920719.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            2502..3248
                     /gene="glnH"
                     /locus_tag="POB34_003857"
     CDS             2502..3248
                     /gene="glnH"
                     /locus_tag="POB34_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HDI5920720.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            3387..4046
                     /gene="glnP"
                     /locus_tag="POB34_003858"
     CDS             3387..4046
                     /gene="glnP"
                     /locus_tag="POB34_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HDI5920721.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            4043..4765
                     /gene="glnQ"
                     /locus_tag="POB34_003859"
     CDS             4043..4765
                     /gene="glnQ"
                     /locus_tag="POB34_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HDI5920722.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            5026..7251
                     /gene="ybiO"
                     /locus_tag="POB34_003860"
     CDS             5026..7251
                     /gene="ybiO"
                     /locus_tag="POB34_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HDI5920723.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(7248..8174)
                     /gene="rlmF"
                     /locus_tag="POB34_003861"
     CDS             complement(7248..8174)
                     /gene="rlmF"
                     /locus_tag="POB34_003861"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HDI5920724.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISSNPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            8450..8710
                     /locus_tag="POB34_003862"
     CDS             8450..8710
                     /locus_tag="POB34_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDI5920725.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            8975..>9550
                     /locus_tag="POB34_003863"
     CDS             8975..>9550
                     /locus_tag="POB34_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDI5920726.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSA"
BASE COUNT         2295 a         2412 c         2380 g         2463 t
ORIGIN      
        1 cctgcggtga aagccagaac tgcggccagt gctgaaagac atgcaatttt tttcataacc
       61 acctcaaatg tgattcaaat aagtcctaag ttttaaatat atcaaaaatt aatgggaaac
      121 tcttcgcgat ttgtgatgtc taacgggcca tttcatgtaa cagaacgttt ccatacaccg
      181 ctatccatct aaatttaaat cactttttca gagaactgcg taagtattac gcatgttttc
      241 cctgtcattc atccagatta ttcctaatca ccagactaat gattccatca atcctggcgc
      301 attttagtca aaacggggga aaattttttc aacaaatgct caaccagcat tgggtatatc
      361 cagtacactc cacgctttac ttaagtctag atatttgtgg gagaaaggat gcctggttca
      421 ttgcgtaaaa tgccggtctg gttaccaata gtcatattgc tcgttgccat ggcgtctatt
      481 cagggtggag cctcgttagc taagtcactt tttcctctgg tgggcgcacc gggtgtcact
      541 gcgctgcgtc tggcattagg aacgctgatc ctcatcgcgt tctttaagcc atggcgactg
      601 cgctttgcca aagagcaacg gttaccgctg ttgttttacg gcgtttcgct gggtgggatg
      661 aattatcttt tttatctttc tattcagaca gtaccgctgg gtattgcggt ggcgctagag
      721 ttcaccggac cactggcggt ggcgctgttc tcttctcgtc gcccggtaga tttcgtctgg
      781 gttgtgctgg cggttcttgg tctgtggttc ctgctaccgc tggggcaaga cgtttcccat
      841 gtcgatttaa ccggctgtgc gctggctctg ggggccgggg cttgttgggc tatttacatt
      901 ttaagtgggc aacgcgcagg agcggaacat ggccctgcga cggtggcaat tggttcgttg
      961 attgcagcgt taattttcgt gccaattgga gcgcttcagg ctggtgaagc actctggcac
     1021 tggtcggtta ttccattggg tctggcggtc gctattctct cgaccgctct gccttattcg
     1081 ctggaaatga ttgccctcac ccgtttgcca acacggacat ttggtacgct gatgagcatg
     1141 gaaccggcgc tggctgccgt ttccgggatg attttcctcg gagaaacact gacacccata
     1201 cagctgctgg cgctcggcgc aattatcgcc gcttcaatgg gttctacgct gacagtacgc
     1261 aaagagagca aaataaaaga attagacatt aattaaattt acatttctgc atggttatgc
     1321 ataaccatgc agaatttctc gctacttttc ctctacaccg tctttatata tcgaattatg
     1381 caaaagcata tttattccta aaattcctgg cgagcagata aataagaatt gttcttatca
     1441 atatatctaa ctcattgaat ctttattagt tttgtttttc acgcttgtta ccactattag
     1501 tgtgatagga acagccagaa tagcggaaca catagccggt gctatactta gtctcgttaa
     1561 ttactgggac ataacatcaa gaggatatga aattatgagt accgctaaat tagttaaatc
     1621 aaaagcgacc aatctgcttt atacccgcaa cgatgtctcc gacagcgaga aaaaagcaac
     1681 agtagagttg ctgaatcgcc aggttatcca gtttattgat ctttctttga ttaccaaaca
     1741 agcgcactgg aacatgcgcg gcgctaactt cattgccgta catgaaatgc tggatggctt
     1801 ccgcaccgca ctgatcgatc atctggatac catggcagaa cgtgcagtgc agctgggcgg
     1861 tgtagctctg gggaccactc aagttatcaa cagcaaaacc ccgctgaaaa gttacccgct
     1921 ggacatccac aacgttcagg atcacctgaa agaactggct gaccgttacg caatcgtcgc
     1981 taatgacgta cgcaaagcga ttggcgaagc gaaagatgac gacaccgcag atatcctgac
     2041 cgccgcgtct cgcgacctgg ataaattcct gtggtttatc gagtctaaca tcgaataaat
     2101 ccatcgctga tggtgcagaa ctttagtacc cgataaaagc ggcttcctga caggaggccg
     2161 ttttgttttg cagcccacct caacgcactt atttagtgca tccatctgct atctccagct
     2221 gattaagtaa attttttgta tccacatcat cacacaatcg ttacataaag attgtttttt
     2281 catcaggttt tacgctaaat aatcactgtg ttgagtgcac aattttagcg caccagattg
     2341 gtgccccaga atggtgcatc ttcagggtat tgccctataa atcgtgcatc acgtttttgc
     2401 cgcatcttga aaaatcaatg agttgcaaaa ctggcacgat tttttcatat atgtgaatgt
     2461 cacgcagggg atcgtcccgt ggatagaaaa aaggaaatgc tatgaagtct gtattaaaag
     2521 tttcactggc tgcactgacc ctggcttttg cggtttcttc tcatgccgcg gataaaaaat
     2581 tagttgtcgc gacggatacc gccttcgttc cgtttgaatt taaacagggc gataaatatg
     2641 tgggctttga cgttgatctg tgggctgcca tcgctaaaga gctgaagctg gattacgaac
     2701 tgaagccgat ggatttcagt gggatcattc cggcactgca aaccaaaaac gtcgatctgg
     2761 cgctggcggg cattaccatc accgacgagc gtaaaaaagc gatcgatttc tctgacggct
     2821 actacaaaag cggcctgtta gtgatggtga aagctaacaa taacgatgtg aaaagcgtga
     2881 aagatctcga cgggaaagtg gttgctgtga agagcggtac tggctccgtt gattacgcga
     2941 aagcaaacat caaaactaaa gatctgcgtc agttcccgaa catcgataac gcctatatgg
     3001 aactgggcac caaccgcgca gacgccgttc tgcacgatac gccaaacatt ctttacttca
     3061 tcaaaaccgc cggtaacggt cagttcaaag cggtaggtga ctctctggaa gcgcagcaat
     3121 acggtattgc gttcccgaaa ggtagcgacg agctgcgtga caaagtcaac ggcgcgttga
     3181 aaaccctgcg cgagaacgga acttacaacg aaatctacaa aaaatggttc ggtactgaac
     3241 cgaaataata acgctacacc tgtaaaacgc actggcagtt ccctctcccc tatggggaga
     3301 ggattagggt gaggggcgca aacccgctcc ggggccatta attaccctga atttgattat
     3361 ttacaccacg gtaacaggaa caacatatgc agtttgactg gagtgccatc tggcctgcca
     3421 ttccgcttct gattgaaggt gccaaaatga ccctgtggat ttcggtcctc ggtctggcag
     3481 gcggtctggt aatcggattg ctggcaggtt ttgcacgcac cttcggtggt tggatagcca
     3541 accacgtcgc gctggtcttt attgaagtga tccgcggcac acctatcgtc gtccaggtga
     3601 tgtttatcta tttcgccctg ccgatggcgt ttaacgactt acgcatcgac ccatttactg
     3661 cggcggtggt caccatcatg atcaactccg gcgcgtacat tgcggaaatc acgcgtggtg
     3721 cggtgctgtc tattcacaaa ggttttcgtg aagcaggact ggcgctcggt ctttcacgtt
     3781 gggaaaccat tcgctacgtc attttaccgc tggcactgcg tcgtatgctg ccgccgctgg
     3841 gtaaccagtg gatcatcagc attaaagaca cctcgctgtt tattgttatc ggcgtggcgg
     3901 aactgacccg tcaggggcaa gaaattattg ccggtaactt ccgcgccctt gagatctgga
     3961 gcgccgtggc ggtgttctat ctgattatta ccttggtgct gagctttatt ctgcgtcgtc
     4021 tggaaagaag gatgaaaatc ctgtgattga atttaaaaac gtctccaagc attttggtcc
     4081 aacccaggtg ctgcacaata tcgatttgaa cattgctcag ggcgaagtcg tggtgattat
     4141 cgggccgtcc ggttccggta aatcgaccct gctgcgctgc atcaacaaac tggaagaaat
     4201 cacctccggc gatctgattg tcgatggcct gaaggttaac gatccgaaag ttgacgagcg
     4261 cctgattcgc caggaagcgg gtatggtgtt ccagcagttt tacctcttcc cgcatctgac
     4321 ggcgctggaa aacgtcatgt ttggtccgct acgcgtgcgt ggcgcgaaca aagaagaggc
     4381 ggaaaaactg gcacgtgagc tgctggcgaa agtcggtctg gcagaacgtg cacatcacta
     4441 cccttccgaa ctttctggtg gtcaacagca gcgtgtggcg attgcccgcg cgctggcggt
     4501 gaagccgaaa atgatgctgt ttgatgaacc gacttccgct cttgacccgg aactgcgcca
     4561 tgaagtgctg aaggttatgc aggatctggc tgaagaaggg atgacgatgg tgatcgtgac
     4621 ccacgaaatc ggttttgccg agaaagtagc ttcgcggctg atctttatcg acaaaggccg
     4681 gattgcggaa gatggcaatc cgcaggtgtt gatcaagaac ccgccgagcc agcgcttgca
     4741 ggaatttttg cagcacgtct cttaataaga cacatttcct gatgcgctac gcttatcagg
     4801 cctacaggat atctggcaac ttattaaaat tgcatgaact tgtaggacgg ataaggcgtt
     4861 cacgccgcat ccggcaaaaa agcccgcacg ttgtcagcaa cctgcttaat atcccttcct
     4921 ccctttcacc cgaaagggag gcacaccaga ttcctctcat ttaaaatcgc ccctcctcca
     4981 gcatctatac ttatcttttt gctctatttt ctcactggag gagtcatgcg gtggatcctg
     5041 ttcatcctct tctgcctgct gggcgcacct gcccacgcgg tatccatacc cggcgttaca
     5101 accacaacga caacggactc aacgactgaa ccggccccgg aaccggatat cgaacaaaaa
     5161 aaagcggcct atggcgcact ggcggatgtg ctggataatg acacctcgcg taaagagttg
     5221 atcgaccagt tgcgcaccgt tgccgctacg ccccctgctg aaccggtacc aaaaatagtg
     5281 ccgccgacgc tggtcgaaga gcaaaccgtg ctgcaaaagg tcaccgaagt cagccgccat
     5341 tatggtgaag ccctttccgc ccgcttcggg caactttatc gcaatatcac cggctccccg
     5401 cataagccgt ttaatccaca aaccttcagc aatgcgctga cccatttttc aatgttagcg
     5461 gtattagtgt ttggttttta ctggctgatt cgcctgtgcg cactgccgct gtatcgcaaa
     5521 atgggtcagt gggcgcggca aaaaaatcgt gagcgcagca actggttgca gcttccggcg
     5581 atgattatcg gggcgtttat tatcgacctg ctgttactgg ccctgacatt atttgtcggc
     5641 caggtattaa gcgacaacct gaatgcaggc agtcgcacca tcgctttcca acaaagtttg
     5701 tttctcaacg cctttgccct cattgaattt ttcaaagccg tactacgcct gattttttgc
     5761 ccaaacgtgg cggagctgcg cccgttcacg attcaggacg agagcgcccg ttactggagt
     5821 cgtcgcctga gctggttaag cagtttgata ggttatggcc tgattgtggc cgtgccgatt
     5881 atctctaatc aggtgaatgt acagataggt gcgctggcga acgtcatcat tatgctgtgc
     5941 atgaccgtct gggcgttgta cctgatcttt cgtaataaaa aagagattac ccagcatttg
     6001 ctcaacttcg cggagcattc gctggccttt ttcagcctgt ttatccgcgc ctttgcgctg
     6061 gtgtggcact ggctggcaag cgcctatttt atcgtgctgt ttttcttttc gttgttcgat
     6121 ccgggcaaca gcctgaaatt tatgatgggt gcaacggtgc gcagcctggc gattattggt
     6181 atcgcagcgt ttgtttccgg tatgttttcc cgctggctgg cgaaaaccat cactctctcg
     6241 ccacatactc agcgtaacta tccggagctg caaaaacggt tgaatggctg gctgtcggcg
     6301 gcgctgaaaa cggcgcgtat tctgacagtc tgcgtggcgg taatgttgct gttgagcgca
     6361 tggggattgt tcgatttctg gaactggctg caaaacggcg cggggcagaa aaccgtagat
     6421 atcctgatcc gtatcgcact cattcttttc ttctcggcgg ttggctggac ggtgctcgcc
     6481 agtttgatcg aaaaccggct ggcttcggat attcatggcc gcccgctacc cagcgcccgc
     6541 acgcgtaccc tgcttacgct gtttcgtaac gcgctggcgg tgattatcag taccatcacc
     6601 atcatgattg tgttgtcgga aatcggcgtc aatatcgcgc cattgctggc aggtgccggg
     6661 gcattaggtc tggctatctc gtttggttcg caaacgctgg tgaaagatat tatcaccggg
     6721 gtatttattc agtttgaaaa cggcatgaac actggagatt tggtgactat cgggccgttg
     6781 accggcacag tggaacggat gtcgattcgc tccgtgggcg tacgccagga taccggggcg
     6841 tatcacatca ttccgtggtc ttcgataacc acctttgcta actttgtccg cggcattggt
     6901 tcggtggtgg caaattatga tgttgatcgc catgaagatg ctgataaagc caatcaggca
     6961 ctgaaagatg cggtagcgga attaatggaa aacgaagaaa ttcgcgggct gattattggt
     7021 gaaccgaatt ttgccgggat tgtcggctta agcaataccg cgtttacact gcgtgtttcg
     7081 ttcaccacgc tgccactcaa acagtggacg gtacgctttg ccctcgacag ccaggtgaaa
     7141 aaacatttcg acctggcggg cgttcgcgcg ccagtgcaga cttatcaggt gctgcctgct
     7201 ccgggcgcga ccccggctga accgttaccg ccgggggaac caacgcttta acgctggcga
     7261 ttgacaaaac ggcggcgctg ctcgtcgttc ataaaggtcc aggcaataaa gcgactctgc
     7321 ttttgcccct gggccatctc ttttttaacc accttcaccg cgcccacgtc cgtcagggca
     7381 cgatacaacg gcggtaagtt ttcaccacga gataccagtg atgtaaacca catcacctgc
     7441 ttcgcgaagc ctttgctctc ttcaatcatc tttttgataa aggtgacttc accgccttca
     7501 caccacaact cctgttgctg gccgccaaag ttcagtgcat cgtctttgtt cagccccagg
     7561 ttacggcgtt tacgctcact acctgcccgt gccgcagcag cggaatcgtg gaatggcggg
     7621 ttacacaagg tcgcgtcgta ttgctcgttt ttatggatga tgccgttaaa aatcgcccca
     7681 ctctcttttt gccgacgcag acgaatggcg cggttaagcc ccggattaga actgataatc
     7741 gcctgcgcac tgcttaacgc ctggctgctg gtttcactac cggtaaaacg ccagccatat
     7801 tcatgtacgc caatcagcgg ataaatacag ttcgcaccaa cgccgatatc cagaatgctg
     7861 gcattcgctg gaattgttcc gcttgcttct gccagtaaat cggcaaggtg atgaatataa
     7921 tccgcccggc ccggtactgg tgggcagaga aaaccgtcgg ggatatccca gttcgctacg
     7981 gcgtaaaaat gggccagcaa cgccttattg agcgccttca ccgccagcgg attggcaaag
     8041 tctacgcttt gctccccggc gggtgtaagc gtgaggaatt gcctgagttc aggattgacc
     8101 tgacaaagcg tggcgagatc gtagcggctg tgatgacggt tgcgcggatg caaccccggt
     8161 ttctgggcgg acatagcatt ctcctttaag ctgcggcgta agatacccgc ataacgcgac
     8221 agggtaaata tctggctgcg tttatcattc cccaaaacct taacatggct atggtttatg
     8281 gcaattcatc ataaaaatca gcattatgca tgtcattgat ttgtcttatt ttgtaaaaat
     8341 atatcgttca aaaaataagc acatctctgt caattttttg cgtcatttgc atcgcgcagg
     8401 cgacctacac ttatcaggca ctacccgttg tatttatgag gatgtcatta tgaaaaagtg
     8461 cctcacacta ctgattgcca ccgtcctgag cggaatctct ctcacggctt atgccgcgca
     8521 accgatgagt aacctggaca gcggtcagct acggcccgcc ggtaccgttt cggcaactgg
     8581 cgcatcaaac ctaagcgatc tggaggataa actggcagaa aaagcgcgcg aacaaggcgc
     8641 gaaaggttat gtcattaatt ccgcaggcgg aaatgaccag atgttcggta ctgcaaccat
     8701 ctacaaataa ccccaccaac gtaactgcat tcgcctctgg atgcagtttt cgtctgtttt
     8761 gtaatcaaaa aacatcaaat atgaactcaa tgtaaataaa tgtatttctt tttcgcgcaa
     8821 tgggtgatag aaaatcgcgc caagtgataa tgcttatcaa aattattatc acttgcacga
     8881 acactatcac gggattaaca gtggcatcgc atccgcagag atgctttctc gtggcagtga
     8941 aaatttcaac atataagaaa aagtcacctg caaaatggaa aacaatcgca atttccctgc
     9001 cagacaattt cattcgctca cgttctttgc cggtctttgt attggcatca cgcctgtggc
     9061 tcaggcactc gccgccgaag ggcaaactaa cgcggatgac acgctggttg tcgaagcatc
     9121 aacgccttcg ctttatgcgc cacaacaatc tgccgatccg aaattctcgc gtccggtagc
     9181 ggatactacc cgcacgatga cggtaatttc tgaacaagtg attaaagatc agggcgcaac
     9241 caaccttacc gacgcgctca aaaacgtccc cggcgtgggt gcgttttttg cgggtgagaa
     9301 cggtaactcc accactggcg acgccattta tatgcgtggt gccgatacct ctaacagtat
     9361 ttatattgat ggcattcgcg atatcggcag cgtctcgcgc gacaccttca ataccgagca
     9421 ggtcgaagtg attaaagggc cgtccggcac cgactacggg cgcagcgcac cgacaggctc
     9481 gatcaatatg atcagcaagc agccgcgcaa tgattccggc attgacgcct ccgccagtat
     9541 tggcagcgcc
//
LOCUS       DAMZET010000161         9548 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.161, whole genome shotgun sequence.
ACCESSION   DAMZET010000161 DAMZET010000000
VERSION     DAMZET010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.161"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..936)
                     /gene="mdtC"
                     /locus_tag="POB34_003864"
     CDS             complement(<1..936)
                     /gene="mdtC"
                     /locus_tag="POB34_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HDI5920727.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTIAQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIP"
     gene            complement(937..4059)
                     /gene="mdtB"
                     /locus_tag="POB34_003865"
     CDS             complement(937..4059)
                     /gene="mdtB"
                     /locus_tag="POB34_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HDI5920728.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALLIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            complement(4059..5306)
                     /gene="mdtA"
                     /locus_tag="POB34_003866"
     CDS             complement(4059..5306)
                     /gene="mdtA"
                     /locus_tag="POB34_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HDI5920729.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            5585..5641
                     /locus_tag="POB34_003867"
     CDS             5585..5641
                     /locus_tag="POB34_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDI5920730.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            5913..5969
                     /locus_tag="POB34_003868"
     CDS             5913..5969
                     /locus_tag="POB34_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDI5920731.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            6242..6301
                     /gene="ibsA"
                     /locus_tag="POB34_003869"
     CDS             6242..6301
                     /gene="ibsA"
                     /locus_tag="POB34_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HDI5920732.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            6522..7181
                     /locus_tag="POB34_003870"
     CDS             6522..7181
                     /locus_tag="POB34_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDI5920733.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIA
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            7178..7939
                     /gene="pphC"
                     /locus_tag="POB34_003871"
     CDS             7178..7939
                     /gene="pphC"
                     /locus_tag="POB34_003871"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HDI5920734.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWAE"
     gene            complement(8004..8465)
                     /locus_tag="POB34_003872"
     CDS             complement(8004..8465)
                     /locus_tag="POB34_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegJ family protein"
                     /protein_id="HDI5920735.1"
                     /translation="MKKILLMLSLLFFTTAGFSEVSDTLVTGGYDKQAMSDAIKHARK
                     ETDKFIEVMNKKDADTFAVKAPITDHGRTEHFWLTDVTYSNGMFIGVISNDPSIVTNV
                     EYGQEWKIKKEDISDWMYTRGDKIYGGYTIDPLLVTYPKEEADELRAKLVR"
     gene            8663..>9548
                     /gene="yegI"
                     /locus_tag="POB34_003873"
     CDS             8663..>9548
                     /gene="yegI"
                     /locus_tag="POB34_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HDI5920736.1"
                     /translation="MKTNIKVFTSTGEMTTLGLELGKGGEGAVYDINEFVDSVAKIYH
                     TPPPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMI
                     YSPAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLI
                     DSDSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFG
                     GRHPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMF
                     QQAFTESGVATGRPTAKAW"
BASE COUNT         2227 a         2468 c         2556 g         2297 t
ORIGIN      
        1 cggaatggtt tcctgcaact ccggtaattt tgcccggatg ctgtcaaccg tctggataat
       61 attggcttcc ggcagcttgc ggatcatcag caaaatagcc ggtttggcat tggtcatccc
      121 ggcgttacgt acatcctgta cagagtcggt cactgtcgcc acatcgccca gacgaaccgc
      181 gccgccgttg ttgtaatgaa taatcagcgg ctgatattcg gcggcggttt ttagctcatc
      241 attggtctgg atctgccagc ggtgagtgcc atcttccagc gccccctgcg gtttacgcac
      301 gttggcattg ctgatggcag tgcgtacgtc gtccagcgag acgccctgat taaacagcgc
      361 ctgcggattc agcccgacgc gtacggcggg cagtgagctg ccgccgacat cgacatcacc
      421 aacaccgtcg atttgcgcaa tcgtcggagc cagctgcgtc gaggcaaaat cgtacagttc
      481 accctgcgaa taagtatcgg atgtcagcgt gaggatcata attggcgcat ccgacgggtt
      541 agctttgcga taggtcggac ggctgggcat cccgctgggc agcaaacttt gtgcagcgtt
      601 gatcgccgcc tgcacatcgc gcgccgcgcc gttgatatcc cggtcaaaat caaactgcaa
      661 aataatacgc gtgctgccga gcgaactgct ggaggtcatt tcactgactc cggcaatgcg
      721 cccaagtgag cgctccagcg gcgtggcaac ggaagacgcc attgtttctg gtgacgcacc
      781 gggcagcgag gcgctgacca taatcaccgg aaaatcgacc tgcggcagcg gggcgaccgg
      841 cagcatacgg aagcccagta tgccgcacag ggtaatggca accgacagta aaatcgtcgc
      901 caccgggcgg taaatgaaga gggcaaaaaa cttcacttac gcctcctctt catgacgggc
      961 aaagcggctt ttggtccaca atgccaggcg gtcgaacagc aaataaatca ccggcgtggt
     1021 aaacagcgtc agcacctggc tgacaatcag accgccgacc atgccgatac ctaacggacg
     1081 acgcagttcc gcgccgaccc cggtactcaa catcagcggc agcgcgccaa gcagagccgc
     1141 cagagtggtc atcaggatcg gacgaaaacg caacagacaa gcctggtaga ttgcctcgcg
     1201 cggcgacatg ccttgctcgc gctcggcagc cagcgcgaag tcgatcatca tgatggcgtt
     1261 cttcttcacg ataccgatca gcaaaataat gccgataatc gcaatcacat ccagttcgct
     1321 accagcaatc agcaacgcca gcagtgcgcc aacccctgcg gtgggtagcg tcgagagaat
     1381 ggtgatcggg tgaataaagc tctcgtacag aatgccgagc acgatataca tcgccaccac
     1441 cgccgcgaca atcagccaga cagtgctgcc cagcgccgac tggaaggcga gggtgctgcc
     1501 ctggaactgc gtggtgatat ccaccggcag attcagcgtc ttttcggtgt ccataatcgc
     1561 ctgcaccgca tcgcccagcg aatagttatc cggcacgtta aaggagatgg tcgttaccgg
     1621 gaactgatcc agatggttga tggagagcgg cgcaaaacgc tgctcaattt tggcaattga
     1681 gcttagcggc accacgccgc cgtcgctgct ggtcaggcga atggtatcca gcgccgcgag
     1741 gcctggggta ttttcggtgt tgtgctccag caccacgcga tactggttgg cctgagtata
     1801 aatagtggaa atcagccgct gaccaaacgc gttgtacagg gcgttatcga catccgccat
     1861 gctgatcccc agacggctgg cgctgtcgcg atcaacattg acatacgcca ccagcccttt
     1921 gtcctgccag tcgctggaga catcagaaag ctgtggcagt tgctggagtt tttccatcaa
     1981 ctgtggcacc caggtactga gcgcatccag tgacgtggcc tgcaaggtaa actggtactg
     2041 ggtgcggctg acctgagtat caatagtcag atcctgcgtt ggttgcagga agagatcgac
     2101 gcccggcact ttatctaccg ccgtttgcag acgggcgatg actttttgca cccgatcatc
     2161 acgttcatcc aacggtttga ggttgatttg taaacgtgca ctgttcagcg acgggttagt
     2221 gccatcaacg ccaacaaatg aggtcaggct ttgcactgcc ggatcctgca aaatcacgtc
     2281 cgcgacctgg cgttgtcgct gggccatatt ggcaaaggag ctggattgcg gtgcctgcaa
     2341 agtgccctga ataatgccat tgtcctgtac cgggaagaaa cctttcggaa tgaacaccca
     2401 cagcagcacg ctaagcagca gcgtgctgag tgccacgctt aaggtcagcc acggatgatt
     2461 cagcactttc gccagtccac gaccataggc ggcgattatc ctgtcgaaca ttttttccga
     2521 ggcacgggag aagcggttct gtttacgcaa cgactcctgg ctgagcatcc gcgcgcacat
     2581 catcggtgtc agggtcagcg acaccaccgc tgagatcaaa atcgctaccg ccagggtaat
     2641 agcaaattcg cggaacagtc gcccgacgat atcgcccata aacagcagtg ggatcaacac
     2701 cgcaatcagt gagaaggtca gcgagataat ggtaaagccg atttcacctg cgcccttgag
     2761 cgccgccgcc aacggttttt cgcctttttc gatatagcgg gaaatgtttt cgatcaccac
     2821 gatggcgtca tcgaccacga atccggtggc gatagttaac gccatcagtg tcaggttatt
     2881 gattgaaaaa tcgagaaaca ccataaccgc gaaagtgccg attaacgaca gcggtacagc
     2941 aacaccggga atgatggtcg ccggaatatt gcgcaaaaac aggtagataa tcatgactac
     3001 cagcgcgata gccatcatca attcaaactg agtatcatcg acggatgcgc ggatattggt
     3061 ggtgcgatcg gaaagcactg tcaccttcac cgatttcggc agactctcag tgagctgtgg
     3121 cagcatctgc cgaatgctgt cggcggtgga gataatgtta gcaccgggct ggcgctgaac
     3181 attcatcaca atggcctgtt ctttgttcgc ccacgcgccg agccagctgt tttctgcacc
     3241 ttgctctaca gttgcgacat cgcccagacg aattggcgcg ccgttctggt aggcgatgat
     3301 tagctggcga tactcttcgg cggattgcat ctggtcgtta gcggaaagcg taaccgcacg
     3361 ggaagggccg tcgaggctac cttttgccga gttaacgtta gcgccggtaa tggcggtgcg
     3421 cacggtttcg ctggtcaggc cgagggcggc aatcgcctga gcgttaagtt tgacgcgaac
     3481 agccggacgc tgaccgccgg aaagcgtcac cagtccgaca ccggaaatct gcgagatttt
     3541 ctgcgcgacg cgggtttcca ccatatcttc cacttgcgtc atcggcatgg cggttgaggt
     3601 gacggcgagc gtcatgatcg gcggatctgc cgggttcact ttgctgtaaa ccggcgggtt
     3661 aggcagatcg ctcggcaaca agttggtcgc agcgttaatc gcggcctgca cttcctgctc
     3721 ggcgacatcg agcggtaatg ttagctggaa ctgcaaagtg ataactgacg caccgccgga
     3781 actttgcgac gacatctgtt tcaggccaga catctgcccg aactggcgtt ctagcggcgc
     3841 ggtaacggca gaggtcatga catccgggct ggcacctggg tagagcgtga ccacctgaat
     3901 ggtcggatag tccacttccg gcagcgccga aacgggcagg gcgcgataac cgataatccc
     3961 ggcgagtaag atcgccacca tcagcagcgt ggtggcgaca ggacgcataa taaaaaggcg
     4021 cgacgggccg cctgtgctgc tcgggggtaa cacctgcatc aggagcgtgc tccttttttc
     4081 gcgtattcgc ggctggtggc tttctcttcc ggagtggtgg cgctctgggc ttccaccact
     4141 tccactttcg ccccttcggt caggcgatca atgccgtctg tcaccacgcg atcgcccgca
     4201 gaaatacctg cacggatcac cactttctga ctgtcctgaa tgcccggcgt caccagatgt
     4261 ttgctgacct tgttttcgct attcagcacc cagacaaaat ggccttcatt gcccatttgc
     4321 agggcggctg ttgggatcac tacggcgttt tgttcggtgt cgactaacat gcgcgcgtta
     4381 acaaactgat tgggaaacag cgcatcatcc tgattattaa agcgtgcttt cactttaatc
     4441 gtaccggttg tggcatcgat ttggttatcg aggcttaaca gcgtgccttc acttaatttc
     4501 ttcgaattgg tgcgatccca ggcttctacc accagcggtt ttccggcttt ttgcgcctgc
     4561 actacagtag cgatatcgct ttccggcagg gtaaagacta aatcgatagg atgcgtctgg
     4621 gtgatcacca caattccggt ggtatcacca ctggagattt ggttaccaac atcaacctgc
     4681 ttgagaccaa cgcgaccatc gactggtgcg gtgatgcggc tccagtcgag ttgcagctgc
     4741 gcgctggcga cgcttgcttc atcagcctta atggtgcctt cggtttcact gaccagcgcc
     4801 tgttgggcat ccagctcctg acgggagacg agattggttt ttgccagttg ctgataacgc
     4861 gccagatcac ggcgggcgtt agcaagcgtg gctttatctt ttgccagttg gccctgggct
     4921 tgtgctaatg caactttgaa ctggctgggg tcaatttctg ccagtaaatc gcctgctttg
     4981 acctgctggc cttcctggaa atgtaacgcc atcagttgac cgtccacgcg gctgcgcacc
     5041 gtaacagtat tagcggcggt aatggtgcca agcccggtga ggtaacgcgg aactgcctgt
     5101 tctacggcgg tcgccgcctg aaccggggct aatgggccgg aacgcatacc acggcgacca
     5161 cccgctggcg attgctgcgc ttgtttcgtc gcccctgggg ctgcactctg ggagtcattg
     5221 cggccttgcc agaaccagaa tgcggcgatg gcggcgataa ccaccacgat tacgattacc
     5281 caacgggatt tataactgcc tttcatcgtt aagagtttct cttcctgaaa cgtttcgcgg
     5341 aatgatacta gtgtagccag ccaataaggg agaaaaatgg aggaattatg aaacactatc
     5401 gggaatcgtc tgaatggcgg gcacatttgc gagcacgcat ccagtaataa cacaggaaac
     5461 tattttatct acgcgtcagc gatagactgc atacagggcg aaaggaggta agccgcagat
     5521 ttcagccgga tgctgaaacg ggaaagcccc tcccgaggaa ggggccttaa ataaggaaag
     5581 ggtcatgatg aagctactca tcatcgtggt gctcttagtg attagctacc cagcatacta
     5641 agactaccag ggcgggggaa accccgctct accctcactc ctgaaagtat gccttcacga
     5701 taagattgtc aatccgcagg ctttgccgtc tgcgatcctg ccagcaaaaa ttctttgcga
     5761 gacgttacgc aataatcaca ctggaaacta ttttatctgc aagtcagcga tagactaagt
     5821 gcatggcgaa aggaggtaag ccgacgattt cagcgggacg ctgaaacggg aaagcccctc
     5881 ccgaggaagg ggccttaaat aaggaaaggg tcatgatgaa gctactcatc atcgtggtgc
     5941 tcttagtcat aagcttcccc gcttactaag actaccaggg cgggggaaac cccgctctac
     6001 cctcactcct gaaagtatgc cttcacgata agattgtcaa tccgcaggct ttgtagtctg
     6061 cgatcctgcc agcaaatatt ctttgcgagt cgttacgcaa taatcacaga ggaaactatt
     6121 ttattcacgc gttagcgata gactgcattc agggcgaaag gaggtaagcc gatgatttca
     6181 gcgggacgct gaaacgggaa agcctctccc ggagaagagg gcttttaata aggaaagggt
     6241 tatgatgaag cacgtcatca tactggtgat actcttagtg attagcttcc aggcttacta
     6301 agaacaccag ggggaggggg aaacctctcc ctaaccctca ctcctgaaat tatgtcactc
     6361 cgcccttagt gtcaatcgac ggctctccgt tcttgcgagg cgaatagcaa aattttctgc
     6421 gccgccgggc ctaattctga aagtttcttc gtttgctctt atttcattat tgcagtcggt
     6481 atgataccgg acctcaataa ttctctaaga aaggatttct tatgagcgaa caaatcacgt
     6541 ttgccaccag cgattttgcc agtaacccgg aaccacgttg cccctgcatt ttgctgctgg
     6601 atgtttctgg ctcgatgagc ggcagaccca ttaatgaact taacgccgga ttggttacct
     6661 ttcgcgatga gctgcttgcc gattcgctgg ccttaaaaag agtggaactc ggtattgtga
     6721 cgttcggccc ggtgcatgtg gaacaaccct ttaccagcgc cgctaacttt ttcccgccca
     6781 tcctgtttgc ccagggggat acgccaatgg gggccgccat taccaaagcc ctggatatgg
     6841 ttgaagagag gaaacgtgag taccgcgcaa atggtatttc gtattatcgc ccgtggattt
     6901 tcctgatcac cgatggcgct ccaaccgatg agtggcaggc tgccgccaac aaagtgtttc
     6961 agggggaaga agataagaag tttgccttct tttccatcgc tgttcagggg gcggatatga
     7021 agacgctggc acaaatcagc gttcgtcagc ctttgcctct gcaaggatta caattccgtg
     7081 aactgtttag ctggttatcc agttcgctgc gttcggtttc ccgctccacg ccgggaacgg
     7141 aagttgtgct ggaagcgcca aaaggctgga cgtcagtgtg agctggcgtc tggtttatgc
     7201 atccgctgtt ggaacgtcgc acattagcgc tgatttacct tgtcaggatg cctgccagat
     7261 gcaggttgcc tggttaaacg atcagcaacc attactggtg atgtttcttg ctgatggtgc
     7321 aggcagcgtc tcgcagggtg gcgaaggcgc gatgctcgcc gtcaatgaag cgatggctta
     7381 tatgtcgcaa aaagtgcagg gtggggaatt ggggcttaat gatatcctcg ccacggatat
     7441 tgtgctgact attcgtcagc gactttttgc cgaggcagag gccaaagaat tagcggttcg
     7501 cgattttgcc tgcacgtttc tgggattgat ttcatcggct aatggcacct tgattatgca
     7561 gattggcgat ggcggcgtgg tggtggattt tggtcacggt ctgcaattgc cgcttacgcc
     7621 gatggtgggt gaatatgcca atatgacgca cttcattacc gatgaagatg ccgtttccag
     7681 actggaaact ttcaccagca ctgagcgtgt gcataaagtt gcagcattta cggatggtat
     7741 ccagcgactg gcgttaaata tgttggataa ttctccgcat gtgcctttct ttaccccgtt
     7801 tttcaatgga ctggcgtcag caacgcagga acaactcgat ttattacctg aattgttaaa
     7861 gcagtttttg tccagcccag cggtaaacga gcgtaccgat gatgataaaa cgttagcact
     7921 ggcgctgtgg gcagagtgag tttctcatgg cggttgaaga caacaattca accgccagtt
     7981 taaacaataa atataacggc tgattagcga actaattttg ctctaagctc gtcggcttct
     8041 tcttttggat aggtgaccaa taaaggatca atggtataac cgccgtaaat tttatccccg
     8101 cgcgtataca tccagtctga aatatcttct ttcttgattt tccactcctg gccatattct
     8161 acgtttgtaa caatactagg gtcattgctg attacgccaa taaacatgcc gttagaataa
     8221 gtgacatccg tgagccagaa atgctcggtt cttccgtgat cggttatcgg ggctttgact
     8281 gcgaaagtat cggcatcttt tttattcata acctcgatga atttatcagt ttctttccgc
     8341 gcatgtttaa tcgcgtcaga catcgcttgt ttatcataac ctcctgtcac taacgtatcc
     8401 gaaacttcac taaagcccgc ggtagtaaaa aatagcaggc tcaacatgag taatatcttt
     8461 ttcatttttt ctccagaatc tctgtgtagt aaaagttagc aaaacctgtg gaagtgaatc
     8521 attatcaggt tggaattgaa ttgtatcctg atgaaattac attatcattt ttattcttga
     8581 gtgggctctt cccatataga aataattata atgggcagtg ttattattca ttgaaatata
     8641 atattgctgc ccttgaaaca ttatgaagac aaatataaaa gtatttacat caacaggtga
     8701 aatgaccact ctcggtcttg aactgggcaa aggcggtgaa ggcgcggttt atgatatcaa
     8761 tgagtttgtc gatagcgtcg ccaagattta tcacacgccg ccacccgcct taaaacagga
     8821 caaacttgcc tttatggctg cgacagctga cgcgcaattg ttgaattatg tcgcctggcc
     8881 gcaggcaacg cttcacggtg gacgaggcgg aaaagttatc ggttttatga tgccaaaagt
     8941 ttctggtaaa gaaccgattc atatgatcta tagcccggca catcgtcgtc aacgttaccc
     9001 tcattgtgcg tgggattttc tactctatgt tgcgcgcaat attgcttcat cttttgctac
     9061 ggttcacgag cacgggcacg tcgtgggtga cgtaaaccag aacagtttta tggtaggtcg
     9121 cgacagcaaa gtggtactga ttgatagcga ctcctttcag attaacgcca atggcacact
     9181 gcatttatgc gaagtcggcg tgtcgcattt tacgccgcca gagctacaaa ccttgtcgtc
     9241 atttgtcggc tttgaacgca cagaaaatca cgataatttt ggccttgcgt tgctgatttt
     9301 tcacgtcttg tttggtggcc ggcatcctta ttctggtgtg ccgcttatct ctgatgcggg
     9361 taatgcgctg gagacggata ttacccattt ccgttatgcc tacgcgtcag ataatcagcg
     9421 acgtggttta aaaccgccgc cacgatcgat tccgctatcg atgttaccga gtgatgttga
     9481 agccatgttt cagcaggcat tcacggaaag tggcgtggca accgggcgtc cgacggctaa
     9541 agcgtggg
//
LOCUS       DAMZET010000162         9386 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.162, whole genome shotgun sequence.
ACCESSION   DAMZET010000162 DAMZET010000000
VERSION     DAMZET010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.162"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            99..1205
                     /gene="mnmA"
                     /locus_tag="POB34_003874"
     CDS             99..1205
                     /gene="mnmA"
                     /locus_tag="POB34_003874"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDI5920737.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            1241..1882
                     /gene="hflD"
                     /locus_tag="POB34_003875"
     CDS             1241..1882
                     /gene="hflD"
                     /locus_tag="POB34_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HDI5920738.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            1886..3256
                     /gene="purB"
                     /locus_tag="POB34_003876"
     CDS             1886..3256
                     /gene="purB"
                     /locus_tag="POB34_003876"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDI5920739.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            3425..4096
                     /gene="phoP"
                     /locus_tag="POB34_003877"
     CDS             3425..4096
                     /gene="phoP"
                     /locus_tag="POB34_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HDI5920740.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHI
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            4096..5556
                     /gene="phoQ"
                     /locus_tag="POB34_003878"
     CDS             4096..5556
                     /gene="phoQ"
                     /locus_tag="POB34_003878"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HDI5920741.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            5632..6753
                     /gene="roxA"
                     /locus_tag="POB34_003879"
                     /gene_synonym="ycfD"
     CDS             5632..6753
                     /gene="roxA"
                     /locus_tag="POB34_003879"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HDI5920742.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPLAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(6802..8028)
                     /gene="pepT"
                     /locus_tag="POB34_003880"
     CDS             complement(6802..8028)
                     /gene="pepT"
                     /locus_tag="POB34_003880"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDI5920743.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVESYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            8278..>9386
                     /gene="potA"
                     /locus_tag="POB34_003881"
     CDS             8278..>9386
                     /gene="potA"
                     /locus_tag="POB34_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HDI5920744.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEV"
BASE COUNT         2320 a         2404 c         2407 g         2255 t
ORIGIN      
        1 atgattggcg attttaactg gccttttaca aagggtgtca tctaaaaggc tgcgtgatag
       61 aatacgccgc cttgaagttc aatgtcgtga gtgatccaat gtctgaaacc gcaaaaaaag
      121 taatcgtcgg catgtccggc ggtgtcgatt cctccgtttc tgcctggctg ttgcaacaac
      181 agggatatca ggtcgaaggc ctgtttatga agaactggga agaagacgac ggtgaggaat
      241 attgcacagc ggcagcggat ctggctgatg cccaggctgt ctgcgacaag ctcggcattg
      301 aactgcacac cgttaacttt gctgccgagt actgggacaa cgtcttcgaa ctgttccttg
      361 ccgaatataa agccggtcgc acgccgaatc cggatattct gtgcaacaaa gagatcaaat
      421 ttaaagcctt cctcgaattt gccgccgaag atttaggtgc cgattatatc gctaccggtc
      481 attacgtacg tcgggccgat gtcgatggca agagccgcct gctgcgtggt ctggacagca
      541 ataaagacca gagctacttc ctttatacgc tcagccatga gcagattgcg caaagcctgt
      601 tcccggtcgg cgaactggaa aaaccgcagg tgcgtaagat tgctgaagat cttggtctgg
      661 tcaccgcgaa gaaaaaagac tctaccggca tttgcttcat tggcgaacgt aaattccgcg
      721 agttcctggg ccgttatctc ccggcgcaac cgggcaaaat cattaccgtc gatggcgatg
      781 aaattggtga gcaccagggg ctgatgtatc acactctcgg tcagcgtaaa ggtctgggta
      841 tcggtggcac caaagaaggt accgaagaac cgtggtatgt ggtggacaaa gacgtcgaaa
      901 acaacattct ggttgtcgct cagggccatg aacacccgcg gctgatgtct gtcgggttga
      961 ttgcccagca gttgcactgg gtcgatcgcg aaccattcac cggcactatg cgttgcacgg
     1021 taaaaacccg ctatcgccag accgatatcc cttgcaccgt caaggcgctg gacgatgatc
     1081 gcattgaagt gattttcgat gaaccggttg ccgccgtgac gccgggccag tctgccgtct
     1141 tctataacgg tgaagtgtgc ctcggtggcg gtattattga gcagcgtctg ccgctgccgg
     1201 tctgattatt atctttactt aaaacaagga agcagtgaac gtggcaaaga attactatga
     1261 catcaccctc gccctggccg gtatttgtca gtcagcacgc ctggtgcaac aactcgctca
     1321 ccaggggcat tgtgatgccg atgcgctaca cgtctcactc aacagtatta ttgatatgaa
     1381 ccccagctcg acgctggcgg tttttggcgg tagcgaagcc aacctgcgcg tcgggctgga
     1441 aaccctgctc ggcgtgctca atgccagcag tcgccagggc ttaaacgccg aattaacccg
     1501 ctacacactc agcttgatgg tgcttgagcg caaactctcc tcagcgaaag gcgcgctcga
     1561 cactctgggc aaccggatca acggcctgca acgccagctc gaacacttcg atttacagtc
     1621 cgaaacgctg atgagcgcga tggctgctat ctatgttgat gtgattagcc cgcttggccc
     1681 gcgcattcag gtcaccggtt cccctgctgt actgcaaagc ccacaagtgc aggcgaaagt
     1741 tcgcgcaacc ctgctggcag gcattcgcgc cgccgtgctc tggcaccagg tcggcggcgg
     1801 acgtctgcaa ctgatgtttt ctcgtaatcg cctgaccact caggcaaaac aaattcttgc
     1861 tcatttaacc ccggagttgt gatctatgga attatcctca ctgaccgccg tttcccctgt
     1921 cgatggacgc tacggcgata aagtcagcgc gctgcgcggg attttcagcg aatatggttt
     1981 gctgaaattc cgtgtacaag ttgaagtacg ttggctgcaa aaactggccg cgcacgcagc
     2041 gatcaaggaa gttcctgctt ttgctgccga cgcaatcggt taccttgatg caatcgtcgc
     2101 cagtttcagc gaagaagatg cggcgcgcat caaaactatc gagcgtacca ctaaccacga
     2161 cgttaaagcg gttgagtatt tcctgaaaga aaaagtggcg gagatcccgg aactgcacgc
     2221 ggtttctgaa ttcatccact ttgcctgtac ttcggaagat atcaataacc tctcccacgc
     2281 attaatgctg aaaaccgcgc gtgatgaagt gatcctgcca tactggcgtc aactgattga
     2341 tggcattaaa gatctcgccg ttcagtatcg cgatatcccg ctgctgtctc gtacccacgg
     2401 tcagccagcc acgccgtcaa ccatcggtaa agagatggca aacgtcgcct accgtatgga
     2461 gcgccagtac cgccagctta accaggtgga gatcctcggc aaaatcaacg gcgcggtcgg
     2521 taactataac gcccacatcg ccgcttaccc ggaagttgac tggcatcagt tcagcgaaga
     2581 gttcgtcacc tcgctgggta ttcaatggaa cccgtacact acccagattg aaccgcacga
     2641 ctacattgcc gaactgtttg attgcgttgc gcgcttcaac accattctga tcgactttga
     2701 ccgtgacgtc tggggttata tcgcccttaa ccacttcaaa cagaaaacca ttgctggtga
     2761 gattggttct tccaccatgc cgcataaagt taacccgatc gacttcgaaa actccgaagg
     2821 gaatctgggc ctttccaacg cggtattgca gcatctggca agcaaactgc cggtttcccg
     2881 ctggcagcgt gacctgaccg actccaccgt gctgcgtaac ctcggcgtgg gtatcggtta
     2941 tgcgctgatt gcgtatcaat ccaccctgaa aggcgtgagc aaactggaag tgaaccgtga
     3001 ccatctgctg gatgaactgg atcacaactg ggaagtgctg gctgagccaa tccagacagt
     3061 tatgcgtcgc tatggcatcg aaaaaccgta cgagaagctg aaagagctga ctcgcggtaa
     3121 gcgcgttgac gccgaaggca tgaagcagtt tatcgacggt ctggcgttgc cagaagaaga
     3181 gaaagcccgc ctgaaagcga tgacgccggc taactatatt ggtcgagcta tcacgatggt
     3241 tgatgagctg aaataaacct cgtatcagtg ccggatggcg atgctgtccg gcctgcttat
     3301 taagactatc cgctttttat tttttcactt tacctcccct ccccgctggt ttatttaatg
     3361 tttaccccca taaccacata atcgcgttac actattttaa taattaagac agggagaaat
     3421 aaaaatgcgc gtactggttg ttgaagacaa tgcgttgtta cgtcaccacc ttaaagttca
     3481 gattcaggat gctggtcatc aggtcgatga cgcagaagat gccaaagaag ccgattatta
     3541 tctcaatgaa catataccgg atattgcgat tgtcgatctc ggattgccag acgaggacgg
     3601 tctgtcactg attcgccgct ggcgtagcaa cgatgtttca ctgccgattc tggtattaac
     3661 cgcccgtgaa agctggcagg acaaagtcga agtattaagt gccggtgctg atgattatgt
     3721 gactaaaccg tttcatattg aagaggtgat ggcgcgaatg caggcattaa tgcggcgtaa
     3781 tagcggtctg gcttcacagg tcatttcgct ccccccgttt caggttgatc tctctcgccg
     3841 tgaattatct attaatgacg aagtgatcaa actgaccgcg ttcgaataca ccattatgga
     3901 aacgttgata cgcaataatg gcaaagtggt gagcaaagat tcgttaatgc tacaactcta
     3961 tccggatgcg gagctgcggg aaagccatac cattgatgta ctgatgggac gtctgcgcaa
     4021 aaaaattcag gcacaatatc cgcaagaagt gattaccacc gttcgcggcc agggctatct
     4081 gttcgaattg cgctgatgaa aaaattactg cgtctttttt tcccgctctc gctgcgggta
     4141 cgttttctgt tggcaacggc agcggtagta ctggtgcttt cgcttgccta cggaatggtc
     4201 gcgctgatcg gttatagcgt cagtttcgat aaaactacgt ttcggctgtt acgtggcgaa
     4261 agcaatctgt tctataccct tgcgaagtgg gaaaacaata agttgcatgt cgagttacca
     4321 gaaaatatcg acaagcaaag ccccaccatg acgctaattt atgatgagaa cgggcagctt
     4381 ttatgggcgc aacgtgacgt gccctggctg atgaagatga tccagcctga ctggctgaaa
     4441 tcgaatggtt ttcatgaaat tgaagcggat gttaacgata ccagcctctt gctgagtgga
     4501 gatcattcga tacagcaaca gttgcaggaa gtgcgggaag atgacgacga cgcggagatg
     4561 acccactcgg tggcggtaaa cgtctacccg gcaacatcgc gaatgccaaa gttaaccatt
     4621 gtggtggtgg ataccattcc ggtggagcta aaaagttcct atatggtctg gagctggttt
     4681 atctatgtgc tctcagccaa tctgctgtta gtgatcccgc tgctgtgggt cgccgcctgg
     4741 tggagtttac gccccatcga agccctggca aaagaagtcc gcgaactgga agaacataac
     4801 cgcgaattgc tcaatccagc cacaacgcga gaactgacca gtctggtacg aaacctgaac
     4861 cgattgttaa aaagtgaacg cgaacgttac gacaaatacc gtacgacgct caccgacctg
     4921 acccatagtc tgaaaacgcc actggcggtg ctgcaaagta cgctgcgttc tctgcgtagt
     4981 gaaaagatga gcgtcagtga tgctgaaccg gtaatgctgg agcaaatcag ccgcatttca
     5041 cagcaaattg gctactacct gcatcgtgcc agtatgcgcg gcgggacatt gcttagccgc
     5101 gagctgcatc cggtcgcccc actgctggac aatctcacct cggcgctgaa caaagtgtat
     5161 caacgcaaag gggtcaatat ctctctcgat atttcgccag agatcagctt tgtcggtgag
     5221 cagaacgatt ttgtcgaggt gatgggcaat gtgctggata atgcctgtaa atattgcctc
     5281 gagtttgtcg aaatttctgc aaggcaaacc gacgagcatc tctatattgt ggtcgaggat
     5341 gatggacccg gtattccatt aagcaagcga gaggtcattt tcgaccgtgg tcaacgggtt
     5401 gatactttac gccctgggca aggtgtaggg ctggcggtag cccgcgaaat caccgagcaa
     5461 tatgagggta aaatcgtcgc cggagagagc atgctgggcg gtgcgcggat ggaggtgatt
     5521 tttggtcgcc agcattctgc gccgaaagat gaataaatat gtccatactt cacgcattac
     5581 gttaagcatc cgttataatc ggttgcagat accagcctgt ggatgcttaa catggaatac
     5641 caactcactc ttaactggcc cgattttctt gaacgtcact ggcagaaacg cccggtggtg
     5701 ttaaaacgcg gctttaataa ttttattgac ccgatctctc cagacgagtt ggcgggtctg
     5761 gcgatggaaa gcgaagtcga cagtcgactg gtcagtcacc aggatggcaa atggcaggtc
     5821 agccacggtc cgttcgaaag ctacgatcat ctcggtgaaa ctaactggtc attgttagtg
     5881 caagcagtaa atcactggca tgagccgacc gccgcgctga tgcgaccgtt ccgtgaacta
     5941 ccggactggc gtattgatga tctgatgatc tctttttctg tacctggcgg cggcgtcggc
     6001 ccgcatctcg atcagtacga cgtgtttatc attcagggta ccggacgtcg tcgctggcga
     6061 gtgggcgaaa agctgcaaat gaaacagcac tgcccacacc cggatctgtt acaggtcgat
     6121 ccgttcgaag ccatcatcga tgaagagctg gagcctggcg atattcttta tattccgcca
     6181 ggattcccgc atgaaggcta cgcgctggaa aatgcgatga actattccgt gggttttcgc
     6241 gcgccaaata cgcgggaatt aattagcgga tttgccgatt atgtgctgca acgtgaactg
     6301 ggcggcaact actacagcga tccggatgtt ccacctctcg ctcatcctgc ggacgttctg
     6361 ccgcaagaaa tggataaact gcgtgagatg atgctcgaat tgatcaacca gccggaacac
     6421 tttaagcaat ggtttggcga gtttatatcc cagtcacgtc atgaactgga tatcgcgccg
     6481 ccggaaccgc cttatcagcc agatgaaatc tacgatgcgc tgaaacaagg tgatgtgctg
     6541 gtgcgcctgg gtggtctgcg cgtattgcgc attggcgacg acgtgtatgc caatggtgag
     6601 aagatcgatt ccccgcaccg tccggcactg gatgcactcg ccagcaacat tgcgctgacc
     6661 gcggagaatt ttggcgatgc gctggaagat ccgtcattcc tcgcgatgct cgcggcgctg
     6721 gtcaatagcg gatactggtt cttcgaaggg taagtttgga tttaagccgg atgcggcatc
     6781 acatgccgca tcccttttcg cttacttccg ttgcgccgtt aactcggcaa tacggacgat
     6841 cacctgcacc gctttttcca taccttccag agtcacaaac tcatgcttac catgatagtt
     6901 gtaaccgcca gtgaacaggt tcgggcacgg taatcccata aacgacaact gcgcgccgtc
     6961 ggtaccgccg cggatcggtt tcagttccgg ttcaatatcg cagtcacgca tcgcctgctg
     7021 ggcgatatcg agaatatgcg gatgctcaac cactttctcg cgcatattgt agtaactgtc
     7081 ttcaatcacc agttcaatgt agcaatcagg atgtaaccct ttgcccactt ttttggcgat
     7141 ctccatcatt ttacgtttac gcgcttcaaa ctgtttacgg tcgaaatcac ggatgatgta
     7201 gtgcatatcg gctcggtcaa cggtgccttt catgctcgcc aggtgataga aaccttcata
     7261 gccttctgtc atttccgggc tttcatccgc cggaacttcc gcatgaatac gtgccgccag
     7321 cgacagcgca tttaccatca ctcctttcgc cgtgcccgga tgaacattgt taccgacaat
     7381 tttgatattg accgatgcgg cgttgaagtt ttcaaactcc agttcgccta cgccaccacc
     7441 gtcaacagtg taagcccagc gggcatcgaa ggcatcaaca tcaaaatgtt tcgccccttt
     7501 gcccacttct tcatccgggg taaaggcgac gcgaatatca ccatgcggaa tgtttttctg
     7561 ttgcaatacc gccagcgcgg tcatgatttc tgcaataccg gctttgtcat cggcacctaa
     7621 caaggtttta ccatcggtgg taatcaacgt ctgacccagt agctgatgca gcaccgggaa
     7681 cataaccggt gataaaactt catcgccgat acccagcgca atatcgccac cgcgatagct
     7741 ttcaacaatt tgcggattaa catttttgcc gctgcaatcc ggtgaggtat ccacatgaga
     7801 aataaagcca atcgccggga tatcgccagg gacgttagcc ggtaacgtcg ccatcaaagt
     7861 gcccttctca cttaaggtca cattgataag ccccatttct tcgagctgct ctttcagcag
     7921 atgcaataac ttccattggc cttccgtgct gggaacctgt ctcacccctg cttttgattg
     7981 ggtatccaga gacacgtagt tcaaaaatcg ctcaagtagt ttatccatgt agtcaccctc
     8041 actttttgtg acaacattat taagaagcaa gaaaagacaa atattgcgtc aggtcacttt
     8101 tacccctgca agcgggaata tttatcagca ttaccttaat gaatataaag ctaagccagt
     8161 aattcacaac aaagattggc gattcaagcg gtttgccgta gaattaccgc cctcattaag
     8221 agtcaccaag gtggttaacc acaaaccccg catcggtaag ccatccgttg cgtttacatg
     8281 ggacagagta aaaaattgaa taaacaaccg agttcgcttt caccgctggt gcaattggcg
     8341 ggaattcgca aatgctttga tggtaaagag gtcattcccc agctggatct gactatcaac
     8401 aatggcgagt tcctcacgct gcttggccct tctggctgcg gtaaaacaac cgttcttcgc
     8461 ctgattgcag gtctggaaac tgttgattcc ggacgcatca tgctggataa cgaggacatc
     8521 acccacgttc cggcggaaaa ccgctatgtg aacactgttt tccaaagcta cgcacttttc
     8581 ccccacatga ccgtgttcga aaatgtggcc tttgggttgc gcatgcaaaa aacccccgct
     8641 gctgaaatta cgccccgcgt gatggaagcc ctgcggatgg tgcaactgga aaccttcgct
     8701 caacgtaaac cgcatcagct ctctggtggt caacagcaac gcgtcgccat tgctcgcgcg
     8761 gtggttaaca agcctcgtct gttgttgctg gatgagtcgc tctcagcgct ggattacaaa
     8821 ctgcgtaagc aaatgcagaa cgagctgaaa gcgttacagc gtaagcttgg tattaccttc
     8881 gtctttgtca ctcacgatca ggaagaagcg ctcaccatgt cagacaggat tgtggtgatg
     8941 cgcgagggtc gtattgagca agatggtaca ccgcgcgaaa tttacgaaga gccgaaaaac
     9001 ctgtttgttg ccggcttcat tggcgaaatc aatatgttta acgccactgt catcgaacgt
     9061 ctagacgagc agcgcgtacg cgccaacgtt gaaggccgcg aatgtaatat ctacgttaac
     9121 ttcgccgttg aaccggggca aaaactgcat gttctgctgc gcccggaaga cttacgtgtt
     9181 gaagagatta acgacgacaa ccacgctgaa gggctgattg gttacgttcg tgagcgtaac
     9241 tacaaaggca tgacgctgga gtcggttgtt gaactggaaa atggcaagat ggtgatggtc
     9301 agcgaattct tcaatgaaga cgaccctgac tttgaccact ctctcgacca aaaaatggcc
     9361 attaattggg tagaaagctg ggaggt
//
LOCUS       DAMZET010000163         9276 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.163, whole genome shotgun sequence.
ACCESSION   DAMZET010000163 DAMZET010000000
VERSION     DAMZET010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.163"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..498)
                     /gene="kduI"
                     /locus_tag="POB34_003882"
     CDS             complement(<1..498)
                     /gene="kduI"
                     /locus_tag="POB34_003882"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDI5920745.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKY"
     gene            complement(785..1966)
                     /locus_tag="POB34_003883"
     CDS             complement(785..1966)
                     /locus_tag="POB34_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDI5920746.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            2221..3450
                     /locus_tag="POB34_003884"
     CDS             2221..3450
                     /locus_tag="POB34_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDI5920747.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            3910..4542
                     /locus_tag="POB34_003885"
     CDS             3910..4542
                     /locus_tag="POB34_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI5920748.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGDKINELFIRSHSVQH"
     gene            4876..5685
                     /locus_tag="POB34_003886"
     CDS             4876..5685
                     /locus_tag="POB34_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI5920749.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTDDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSYREVNRD"
     gene            5678..6160
                     /locus_tag="POB34_003887"
     CDS             5678..6160
                     /locus_tag="POB34_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920750.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(6193..6273)
                     /locus_tag="POB34_003888"
     CDS             complement(6193..6273)
                     /locus_tag="POB34_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920751.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(6309..6734)
                     /locus_tag="POB34_003889"
     CDS             complement(6309..6734)
                     /locus_tag="POB34_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920752.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAENVLTQTT
                     SWTLKSRYVNVNSVNKVNVKSKVKNSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
     gene            6928..7418
                     /locus_tag="POB34_003890"
                     /pseudo
     CDS             6928..7418
                     /locus_tag="POB34_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7643..8134
                     /locus_tag="POB34_003891"
     CDS             7643..8134
                     /locus_tag="POB34_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDI5920753.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
     gene            8469..>9276
                     /locus_tag="POB34_003892"
     CDS             8469..>9276
                     /locus_tag="POB34_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HDI5920754.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDGLYTYPMAA
                     TNFCNDHISQNSFLSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLFLIASNHLPVDE
                     LHNTSQFIIDNILQTVHKPERSVRLAKQDQGYKNHYLSDEMLAGKKELYDF"
BASE COUNT         2982 a         1848 c         1726 g         2720 t
ORIGIN      
        1 atatttgtta atagtgcgac ggttactggt gaggttatcg cctaacgtga ctggagatac
       61 ttcgtccggg gtgacttttt tggtgggata cgtcgtatgc gcgggtgcgc aattgtaata
      121 aaactttgcc ggagtggcgg tatcaatact ggcaaagaca acttcttttg cacctttacc
      181 cacatacagg gcgtcgcggt gaccgatttc atagcattgg ccatcgacag taatcgtacc
      241 ggcaccgcca atattgataa cacctaactc gcgacgttca aggaaatagc ttacgccgag
      301 ttgtttacca acttccccgc caacggaaac cgttttagtt atcggcataa tgccgccaac
      361 aataattcgg tcaatgtggc tgtaaaccat ggtgtactca tcggcgacaa atacctcttc
      421 aaccaaaaat tcattgcgca gcccttgggt atccagcgtt tttgcgtgcg cactgtggat
      481 gctctgtctt acgtccacat caacctccga taatcagtga agcgtgaaaa tagaaaacga
      541 tagtgaaaca ctgtttcgtt ttctctataa agggatgata tgcccatggg ctagggtttt
      601 caattaaaaa taaaacaatg tttcattttt aagttaagga ttaaaaaaag tgcagtgtcg
      661 atcacgaata tgtcatgaga atggaaaaat gagggagccc agaaaatgta tgtcgacttg
      721 ccagccatca ttattcaaca gattaatgac ggcagatatt tatcggtaag gaggaaatgc
      781 agagctactc gtcacgttca atggtcaatg ccacgccctg gcccccgccg atacaaagcg
      841 ttgccagccc tttgcgggca ttacgtttca ccatttcatg aaccagagaa accaggattc
      901 ggcaaccgga agcgcctatc gggtgaccga gtgcgatcgc gccaccattg acattgaccc
      961 gacgctcatc ccactcaagc atcttgccaa ccgaaagcgc ctgtgcagca aacgcttcat
     1021 tagcctcgat aagatcgaca tcagccaact gccagcctac acgctccagg caacggcggg
     1081 tcgcatacac cggcgcaatt cccatcaatg ccggatctac accaacgctg gcaaatgcgc
     1141 ggatgcgggc cagcacgggt aaattcaacg ctcgtgcttt ggcttcgctc atcatcatta
     1201 ccgcagctgc gccatcgttt atggatgatg cattccccgc ggtcacagaa ccgagactat
     1261 caaatgaagg atttaaacgg gctaaacctt ctgcactggc gtcagtgcgt ggctgttcat
     1321 cggtatcaac aaccaacgtc tgaccgttac tttgggtcat taccgggacg atctcatctt
     1381 taaatcgtcc ggcgtcaatc gccgctcgcg ctttttgttg cgagctaagt gcgtaagcat
     1441 cctgcaattg acggctgatg ccatattcgc gagccagatt ttcggcggtg acaccaatat
     1501 gataatcatt gaaggcatcc cataacccat catgcacaag actgtcaacc agctggctat
     1561 tgccaagctg tgcaccggtg cggctatcag tcagaacatg tggtgcgcgg ctcatgtttt
     1621 cctggccacc ggcgatgaca atatcagcct cgccacactg tatcgcctga gtagccagat
     1681 gcagtgcttt aagtccggaa ccgcagacgt cattaatcgt gattgcagaa acgctattag
     1741 gcagaccacc tttaatagcc gattgccttg ccggattctg ccctgcccct gcagtcaaca
     1801 cctgaccaag aattacttca tccaccgcat atgcaggaac gccggtacgt tctattaacg
     1861 ctttcacgac cagactacca agttccacgg cggaatgacc cgctaacgca ccgcgaaagc
     1921 agccgatagg tgtccgtaac gccccgacaa tcacaacgtc tttcatcaca acctcgcacc
     1981 aattaacagt gcaatagtaa atgattgtta attacagtta tcttaattgt ttaaaaaaag
     2041 tgatttttat cacaaaggaa atatgcctga gcagtagtca gagtcataac tggcacgtaa
     2101 ggtttgcaac cactaaccca ccaatagagg ggtagatagg gcgttaatct cccatactta
     2161 acctggttta tggtaaattg ccctccattt tgtttaattt gtagatgata cgttcagata
     2221 atgtctaata tttggtctaa agaagaaact ctgtggagtt tcgcgctcta cggcacagcc
     2281 gttggtgcag gcacgctctt ccttcctatt cagttaggtt cggcaggggc tgtggtcctg
     2341 tttattactg ctctggtcgc ctggccttta acatattggc cacataaagc cttatgccag
     2401 ttcatcctct catcgaaaac atcagcaggt gaagggataa cgggcgcagt aacacactac
     2461 tatggcaaga agattggtaa tctgattacc acgctgtact tcatcgcctt ttttgtcgtc
     2521 gtgttgatat atgcagtggc aattaccaac tcacttacgg aacagctggc aaagcatatg
     2581 gttattgatc ttcgcatccg tatgttggtg agtctgggtg ttgtattaat cctgaatctc
     2641 atttttctga tgggacgcca tgcaaccatt cgggtaatgg gttttttggt attcccattg
     2701 attgcctatt tcttatttct ttccatttac cttgtcggta gttggcaacc tgatctatta
     2761 acgacccagg tagagttcaa tcagaatacc cttcaccaga tatggatatc gattcccgtg
     2821 atggttttcg cctttagcca tacgcccatt atttctacgt ttgccataga cagacgtgaa
     2881 aaatatggcg aacacgctat ggataaatgc aaaaaaatta tgaaagtcgc ttatctcatc
     2941 atctgcataa gtgtactgtt ctttgtcttt agctgcctgc tttctattcc accttcgtat
     3001 attgaagcgg ctaaagaaga aggggtcacc attttatcgg cgctttctat gctgccgaac
     3061 gccccagcat ggctgtcaat ttccgggatt attgtcgcag tagttgcgat gtcgaaatca
     3121 ttcctgggta cgtactttgg cgttattgaa ggtgccacgg aggtcgtcaa aacaacacta
     3181 cagcaggttg gtgtaaagaa aagtcgtgca tttaaccgcg cactatcaat tatgttggta
     3241 tcgctgatca ccttcattgt ttgttgcatt aacccgaacg cgatttcgat gatttacgcg
     3301 atcagcggcc cgctcattgc catgatactt ttcatcatgc ctacgttgtc aacgtatctg
     3361 atcccggcgc ttaaaccctg gcgttccatc ggaaatctga ttacgctgat cgtgggtatc
     3421 ctgtgcgtat cggtaatgtt ctttagctga tatccattgt gaatggttca aaattgctac
     3481 aattttgaac cattccagtt ttcataatgt tcattcgtta ttattccgca ctatcacttc
     3541 cctcatcata tcctagtatt atcagttagt taaacacaca aagagatctg aacatcttaa
     3601 ataagactat ttaagatgca taacttagat tcgcaagata tcttctggtc attcaaaaac
     3661 aatttgcgat atagatattc tcataccatg agatagttat ctgaaccacc ctcaaccagg
     3721 taagattaaa tcagtgaaac ataatcatat ttaataacga gaaatgcatt ttaaaatgtc
     3781 ttaatcagaa ttatagcaaa tacagattaa tcgcaatttt ttccagcaaa attacaaaat
     3841 catggtgtat tgacatcatc aatcaccaat gaaattatgc aattatatac ggatagggag
     3901 gttcttaaca tggggctttg tagtcgttat aaaagtctta catgcaatag ttgctcaatg
     3961 cattgccaaa taatgccaga agagtcaccg cgtttgcagt actgcgcaaa ctcgtgtttt
     4021 tgtatgtggc ccgaagaaag ttcatatttt aatcgtggtg tggtagaagg tatcttaaca
     4081 aaaaaccaca atgcgagatt aagcggatat atttttgtcg atttttcagt aagtttttta
     4141 cgtctattcc ttgaaaaaga ctggattgac tatcttgcaa gtactgacat ggggattgtt
     4201 ttagtcagcg accgcaatat gcaatcgtta gctaattact ggcgaaaaca caactcagca
     4261 atatctgctg tcatatataa tgatgatggt cttgatgttg ccaatgaaaa gatcagacaa
     4321 ctatttattg gtcgttattt atcatttacc agaggtaaca cattaactca gatggaattc
     4381 accataatgg ggtatatggt ttctggttat aatccatatc agattgctga agttctggat
     4441 atggatatcc gtagcatcta cgcgtacaag caacgaatcg aaaagagaat gggtgataaa
     4501 ataaacgaat tatttattcg ttcacattcg gtccaacatt gatactcaac gaccaggcag
     4561 aatcatactc tgtttatacg ggcatttatt gaaatgtccg tattttataa ataggccgtc
     4621 ccctcaagag taaacaccat agtatttaaa accatcacac gaaaaattca gaagcattac
     4681 gaaatatgaa tttacaaaca atagtggcat aaatgttaac catgttaatt tacgtaaagt
     4741 tttacgttgc aacattaaag cctcatttca atcatcatga taaatataaa attaatatat
     4801 atttatgccg taaataccaa tatacttagc aaactatgtg atctccattt cgattgattt
     4861 agtgtttatt gacgtatgta ctggattatt aacgataata tcgagttctg gcctgagcac
     4921 cgaaaattaa tatcggtaca taacgccgat cttaacgtcg ttctgacaac gccagccagt
     4981 cgatgtttat cacttctact tgaagctttt cctgatgtgg ttgcacaaca agattttttc
     5041 accagagtct gggaagaaga aggtatgcgt gtgcctacta acacgttata tcagaacata
     5101 tccattatca gacgcggatt tcgcgctgtt ggtgatacta cccactcgct aattgcaacc
     5161 gtgccgagaa gaggattcaa gatccataat gacattaaca tacaaaatca tgtaataaac
     5221 tcgtcaacag acgcacatac acacaatgcc ccacctgcca taaaagttaa tgcgggatac
     5281 aaagagagca ttggtggcgc aaagaatttc aataacaaaa tcctcaaaca tataaaaagt
     5341 catctaatta tgttgagcgc atttgtcata ggcgcatatt ctgcatattg gctatggaat
     5401 aataatcaac cgaagccatt tttcaaggat tacaagactg ttgcggaaat taatggctgc
     5461 cattttaatg taacagacga tacaattgat gggttgaaag agttcgataa atataaaaca
     5521 cggatactgg attcggggat taactgtaaa aaacatccgt ggttatactt ccctcttgca
     5581 aaatcctcac cggggatgat tgttatggca tgcaataaaa actataacca acatgaagtg
     5641 gcagactgtt taaccctgtc ttaccgtgag gttaatcgtg attgattata aaaaaaactt
     5701 attatttatt ttagttttta tcagtgggtt tattctcttc actgtctatt cttacactgc
     5761 agaaaaaatg atatataacg aaacatgcac tgcaaattgg gtaattttta atgaccaggg
     5821 acgagcaaat cttaccatcg actttatgta taacaaaaaa aataaaacag gaactgttgc
     5881 gttgagtgga acctggcaac aaggtaacag agaaagtaaa tcaatacggc gaaatattga
     5941 atacacatgg attgaaaact atgacacagc ccatttaaca tcaaagaaag ttaataagtt
     6001 tgagattatg gatcaagttg atgatgatag acttgcacaa cttattcctg atttttatgt
     6061 ctttccagaa aaaagtgtaa gctataatat actaaagcaa ggtaagcatg cttttatttt
     6121 gagcattggt aacagagcaa taatgcattg tgcaaggtaa actaaaatag ataacaaaaa
     6181 gatggaacct cgttaatcat aaaacaataa aattaaatat tctcgcagta tatggcagtc
     6241 taaagcatca aagttttgat caacatcttt cattttagac atctccttgc aatgtaaaat
     6301 atcatgtatc aacaacatct ggtgcaaatc catgagtcga actcgacatt catcttagct
     6361 cgattattat tccttcgtac agtcgatgta aacaatacag aaagaggatt attaagaacc
     6421 agtttactca gtgaatcaat agaggaaagg actaacgttt ctttaaaaga attgatttca
     6481 tcatctgtta aactaaactc atcattgaca gatcgtgaga tataactgtt tttaacttta
     6541 ctctttacgt tgactttatt gacagaatta acattcacat atcttgattt taatgtccat
     6601 gatgttgttt gcgtgagaac attctcagca ttaaggaatt tttttaacgg aatccttggt
     6661 ttctttttcg agtccgaatt tacaatatca tgcatataga ccatttcatg aatctgcgaa
     6721 aattcaatgt ccattgtata cctcacattt ttaccgtgac tcgatgttac tgttcaataa
     6781 tcaccttcca tcaatactaa aattaatacc cctaatgtgc cgataacaaa tatagtcatt
     6841 ctacgtaacg tctccataag gtgatatttg acattatcag aagttgcgaa ttcggatttt
     6901 gctctaatca aatgagagaa atgtaaaatg aagccacgaa atattaataa tagcctacca
     6961 ctgcaaccat tagttcctga tcaggagaac aaaaataaga aaaatgaaga gaaatccgtt
     7021 aatccagtta aaatcacaat ggggtctggt ttaaattata ttgaacaaga atctcttgga
     7081 ggaaaatatc taacacatga tttgtcaata aagatagcgg atatttctga agagataatt
     7141 cagcaagcaa tattatctgc tatgagcata tataaatttt cgataacaga tgatttaatg
     7201 agtatggctg taaatgaact cataaaactg accaaaatag agaataatgt agacctgaat
     7261 aaattcacta ctatatgcac agacgttcta tccccccgcg tcaccagaca taataaagaa
     7321 aaaaaacaaa cgacattcta cccttctcaa aaatcccctt tttaattttc attgaaaaaa
     7381 tggcaattga tactatgaat ccaggtcata cacattaagg catttatgta atgtcgtaat
     7441 taagataact aataaggtga atattagtaa aacaactaat gatgttatta gtttgtagtg
     7501 aacagtactt ttaccaataa tgaaaaatat accataagca atgcaagctt aaaactcata
     7561 atttaaaaaa gataaatata aaaatatcaa tgagtcaatc aataataatt tggcatcacg
     7621 agacacatca cagaggaata ttatgagcac agaaacaatt gaaatattca ataatagtga
     7681 tgaatgggca aatcaactaa aacacgcatt atcgaaagga gaaaatctgg cattactaca
     7741 tggtttaact cctgatatcc ttgatagaat atatgcatat gcattcgact accatgaaaa
     7801 gggtaatata acggacgcag aaatttatta taaatttttg tgcatttatg cgttcgaaaa
     7861 tcatgagtat ctaaaagatt ttgcatcagt atgtcagccc aaaaagaaat atcaacaagc
     7921 atatgacctt tacaaactaa gttacaatta ctccccgtat gatgactatt cagttattta
     7981 tcgtatgggt caatgtcaga ttggggctaa aaatatcgat aacgcaatgc aatgtttcta
     8041 tcacattatt aacaattgtg aggatgatag tgttaagagt aaagcgcagg catatattga
     8101 actcttaaac gataattcag aagataatgg ctaagcatta caatcatttg gttaaatttg
     8161 ctggttatag tgcagttaac cactataacc agcacctctt atgttttctt aaatcagcat
     8221 cttaaaacag ttaagagcat actataaatc attacttttc aatgcgttag agaacaatct
     8281 actttataaa aattgaataa ggctatttca gattacatca ctgaattcct aagataaatt
     8341 agtgaacaca accatagata tgtcgaatgt aatttcctgt agcattaatg acacataaat
     8401 atgaatagcc ataatttcta ttgcttaagc aggatgcaag aaaccaattt tttcatagag
     8461 gttaactaat ggacttagaa aataaattct catatcattt tcttgaggga ttaacgctca
     8521 cggaagatgg aattcttact caaggaaatg agcaagttta tattccacag aaagagttag
     8581 gtgtattaat agtattactt gaatccgctg gtcatgttgt actgaaagat atgatcatcg
     8641 aatcagtatg gaaaaatatt attgttagtg acgagtccct gacaagatgt atctattctt
     8701 tgcgctgcat ttttgaaaaa attggctatg atcgttgcat agaaacaatc taccggaaag
     8761 gttatcgttt cagcgggcag gttttcaaaa ctaaaattaa tgaagataat acttcagact
     8821 attccatagc tatattccct ttcactactt cattgaatac actggatcca ttaatactta
     8881 atcaggaatt agtgcaaatc atttcaaata aaaaaatcga tggtctctat acctatccga
     8941 tggctgcgac aaatttttgt aatgatcaca tatctcaaaa ttcattcttg agcagattca
     9001 agccagatta tttcgttaca ggaagaataa accagaataa tgcagtgaac actttataca
     9061 ttgagttgat cgacgctaaa aaccttttcc tcatcgccag taatcatctc cctgttgatg
     9121 aactacataa tacatcacaa tttattatag ataatatcct tcaaacggta cataaaccag
     9181 aacgatctgt aagattagct aagcaggacc aaggatataa gaatcattat ttatcagatg
     9241 aaatgttagc cggaaagaaa gaactttacg acttca
//
LOCUS       DAMZET010000164         9218 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.164, whole genome shotgun sequence.
ACCESSION   DAMZET010000164 DAMZET010000000
VERSION     DAMZET010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.164"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..563)
                     /locus_tag="POB34_003893"
     CDS             complement(<1..563)
                     /locus_tag="POB34_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDI5920755.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDS"
     gene            complement(577..2172)
                     /locus_tag="POB34_003894"
     CDS             complement(577..2172)
                     /locus_tag="POB34_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDI5920756.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLHIVQEGRVNKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(2317..3075)
                     /gene="aroD"
                     /locus_tag="POB34_003895"
     CDS             complement(2317..3075)
                     /gene="aroD"
                     /locus_tag="POB34_003895"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDI5920757.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(3106..3972)
                     /gene="ydiB"
                     /locus_tag="POB34_003896"
     CDS             complement(3106..3972)
                     /gene="ydiB"
                     /locus_tag="POB34_003896"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HDI5920758.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(3984..5249)
                     /locus_tag="POB34_003897"
     CDS             complement(3984..5249)
                     /locus_tag="POB34_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5920759.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(5476..6690)
                     /locus_tag="POB34_003898"
     CDS             complement(5476..6690)
                     /locus_tag="POB34_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5920760.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYLCTFPPHPGHRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(6790..7146)
                     /locus_tag="POB34_003899"
     CDS             complement(6790..7146)
                     /locus_tag="POB34_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HDI5920761.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQRVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(7282..7389)
                     /gene="rprA"
                     /locus_tag="POB34_003900"
     ncRNA           complement(7282..7389)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="POB34_003900"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(7575..8687)
                     /gene="ydiK"
                     /locus_tag="POB34_003901"
     CDS             complement(7575..8687)
                     /gene="ydiK"
                     /locus_tag="POB34_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HDI5920762.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            9076..>9218
                     /locus_tag="POB34_003902"
     CDS             9076..>9218
                     /locus_tag="POB34_003902"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920763.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSI"
BASE COUNT         2589 a         2241 c         2112 g         2276 t
ORIGIN      
        1 gagtcgaccc accacagggt gaaggctttt ttgggatctt tcggttgcgg atcgcgcgcc
       61 aacaccagca tatacgggta ctctttcgca ccggtaataa aggttttttg tccgttgatg
      121 tagaccttgc cgtttttacg cgtgtaagtg gtagtggcac tgttgttatc tgagccagca
      181 cctggttccg tcaacgccag ggcataggcg ggatcacccg tttccagggt gctttctgcc
      241 gttttacgta gctgctctgc agaaccgaaa cggcgcatac tgtgaataca ttgaccgttg
      301 gtgatcaaaa atgccggagc accgcatttt gacacttcca tcagcgccag catttgggtg
      361 acgtaatccg cagggatacc accaaattct tccggcacgc caagcatgga aataccgtta
      421 tccgccagcg cccgcataaa ctcacgcgga tatgtcccgt tttgatcgca ggtgcggaaa
      481 tactcttccg gaaagttagt cgtaatcagt tcgcgaatac tggccagcag cagttcttgt
      541 tcttcagtta aagaaaaatc catcattata ctcctattaa tgagccgctt caggcaggac
      601 aaaacccatc gccgcatcga taaataatct ttcgtccatc agtttgagtt ctggcgaaat
      661 cactggggtg aagtccattt tgtcgagaat atctttttgt aaatcgacgc caggggcgat
      721 ttcgattaaa tgcaggccgt cttctttcag cgtgaataca gcacgttcgg tgatgtaacg
      781 aacatccagt ccccgttcga gcgcgatttt tccgctaaaa gtaatttccg gtagttcccg
      841 aataaattta tttacccgtc cttcctggac gatatgcaat ttgccatcgg caatttctgt
      901 ttttaaactg cccgcagtta acgtgccgca gaaaataatt ttcttcgagg tggcactgat
      961 atcaataaat ccaccggtac ccatgatttt gccattgaat ttatgcacgc cgacgttacc
     1021 gtgctggtcg acttcagcaa aactcaaata acaaacatcc agaccaccac cgtgataaaa
     1081 atcaaactgg gacgtcatat ccagaatggc gcgggtattc acgttcgcgc caaaggcgat
     1141 accctgtgaa gtaattccgc caatcggacc tgtttctacc gtcagaataa agtcatcagc
     1201 acaaccttct tcccgggcga ccaggccaat accgtcagca ataccgacgc cgacattccc
     1261 caccgcgcct ttacgcattt cgaataatgc acgccgcgca actaatttac gttggtttag
     1321 gggcaggcta agtttggtgc tgtcatcaag ggtgaagtca ccagaaataa aacggttaac
     1381 cggcgcaccg ccatacagtt ggctttgatc cggatcgacc accacaatat ccaccagata
     1441 acccggaata cggacagatt taggatgcag cgtggctttc ttaaccattt tctgcacctg
     1501 catcatcaca ataccgccgt tattgtgcac cgcctgggca ataaccaatg cgtcgagata
     1561 catcacctca tcttcaaaag tggcgtagcc ttcactgtcg caggtggtag cgcgaatgaa
     1621 ggcgatatct ggcgcaatcg ctttgtaata gagatattct ttgttatcaa actcgaccag
     1681 tttaatcagg tcttctttcg tgacttcatt cagtttgccg ccttgctggc gtggatcgac
     1741 aaatgtcccg atgccaatat cactaataat accaggctgg tgggctgcgg cggcgcgtaa
     1801 ggtttgtgta agtacacctt gtggatagtt ataagcaata attttatttt gttctgcgag
     1861 ttcagaaata cgcggcgatt gtccccagtg accacataat gcccatttca ccagaccttc
     1921 ttgcgccaga ggactaatac cacggtcggc gcgatcgcca agccctgtcg gactaataat
     1981 cgataaatta cgtggcgttt gagtctgttt atatttatca gcaagagcag taattaacgt
     2041 ggtggcttcc agaataccgc cgccagcgcc taacacacaa agtgttgctt cgtcgggaat
     2101 ataattcacc gcttcctgtg ccgacaggac cggcactcgt ccattaatac gaggtggttt
     2161 tacaggtttc attgttaatt cctgattcag gtgtgatttt ccctacgcca gatggataat
     2221 tagcgcacag agactcacga aatattttta ttaaatacgg ctgaatatat ttttttagtt
     2281 cggcggggga gggtattccc gccgaaatat tattgcttat gcctgatgta aaatagttaa
     2341 tactgtgcgc aaatcattta ccgagatttg ccctggcgca gaggcttttt ttaccgcacc
     2401 aaaagttgcc gccgagccaa atacttcacc agccagacga gaaattacgc cagtttttgc
     2461 cattgacatc gtgatgattg gacgatcggc atactgctcc tgcatctcca gggtcgcggc
     2521 aagcaacgtc agcacatcgc tggtactttg cggcatcagc gcaatcttag gaatatcggc
     2581 gtcgaaggac tgcattttgc gcagacgggc aatgatttct tcggcttccg gcgttttatg
     2641 gaagtcatgg ttggacatga caactttcac atcatgcgcg tgggcgtagg cgacggtttc
     2701 tttaacctga tcatcgccgg taaataactc cagatcgatc atatcaacca ggccgctgtc
     2761 gatggctgca cgattgagag caatataagc ctcggtggaa atcgcctgct cgccgccttc
     2821 tttggcactg cggaaggtaa acagcagcgg tttttctggc atggtttcac ggagaatttt
     2881 tgccgccgcc atgacagact ccacattgga gaggtcggca aagtggtcca cacgccattc
     2941 cagaatatca aagtccgctt cacgataggc gagagcttcg gatttcacgc gggcgatatc
     3001 tttcgccatc agcgagacga tgattttagg tgcgcccgta ccaatgacga gatcttttac
     3061 agttacggtt ttcatttttt accctttctg cacgcggtca gcctgtcagg caccgaaccc
     3121 catgacctgt ttaacatatt ccagagggaa atctttgcca gtccataacg tgaactgttc
     3181 agccccttgc cacaacaaca tgccgtatcc atcaatcgtt ttgcaaccag cttgttgcgc
     3241 ctgctgcaat aacttcgtca tatgcgggtt atacacgcat tcagtgacca gcagtcccgg
     3301 atgtaacaga ctgatatcat taactaatga ttcattctca aggggtttca tacccacttt
     3361 tgtgccattg gttaaaatgt cggcggaagc cagggcttca gcaaaggctt gttgatcggc
     3421 gagatcggta accgtgacga cacaatcggt gttttcgtta acccgctgcg cgaaggcgag
     3481 ggctttatcg aagaactcat cccgacggtt aaagagttta atttctttta aaccttcaat
     3541 tgccccctgc gcgccaatcg ccgttgaggc acctccggcc cctaacagca ccattgtttt
     3601 gcctttgata tcaaaaccgc tctctttaat ggcgcgaata tggcccgtgc cgtcggtgtt
     3661 atagccgcgc aggtagccat catcattaac gatggtgttg atggcaccca ccagtttggc
     3721 tgctggcgtt aattcatcaa catattcaca cgccagttgt ttgttcggca ttgatacacc
     3781 agttccgcgc atttttagag cttttaatcc ttcaattgct ccgggaaagc tatcgttatc
     3841 cacttcgaag gccatatagg taaatggcaa tcccgctttt tctaaggctt tattctgcat
     3901 ttcaggcgat aaactgtggc ggataggata agccatcaac ccaatcaatt cgtattttgc
     3961 ggtaacatcc ataattaact cctttaacct ctatgcttaa ttgtgtttag ccgggtgcta
     4021 aatttacgct cgccaaaccg cacatcattc tcaggaataa tgaaaacgcg gtaatagcgg
     4081 ataaagacaa taattgcggt aatcagggcc agcagcgcga aagtaaaatc gagaacaatg
     4141 atatattgaa ggccgatgtt cgacagataa ccggtaatca gcggaataac aaagttagcc
     4201 agtccaccca tcatcatata aatactggtg actttggctt tgctttttgg gaaaaactct
     4261 gacatgaccg aaacaccgag ctgtaaaatg ccgccagctg ctgagaaacc gataacaaag
     4321 gcgccggcat tacacaccag tggagaaggg tacaggtaga taatggctgc tgttattgtt
     4381 gccagtgcag agttaaatac attagcccag atgggccgga ccattttttt cagtagtgcg
     4441 gcaaaaataa agacacagac caacgagccc atactgtaat aagagatggt ttttaatgcc
     4501 tcagcttctg acataccagc aaaagccatc gcatatttgg gcatccacac cacaatcaca
     4561 taaaaggtcg agaatgcggc tacaccgaac agtaccgatg aaacaccttc cagccagact
     4621 aacggtttgc tgttcatttg cggtaattca ttagctacgc tggcgtccac caactggctg
     4681 gggaatttgc ttttcaacag catcagcgtg atcagtacaa acagaatacc cggaataatc
     4741 agcccatagc cgtaccagat attattgagc aacatatagc tcaccagcat tgggtagaac
     4801 atttgcccaa atgacaccat cgctttaacc agtatgaccg ccgaaccaga ggctttcgga
     4861 aagcattcca tgagcgcggg gtagccaccc gtatccagcg ctgagttagc gatacctacg
     4921 cacactgcca gaccgtaggc gagagttaaa ttcgggcaag cgggaatacc aaagaagaat
     4981 agcagataca ttattactgc cattaatatc accgcccgac gaccaaactt atcggagatc
     5041 acaccgaaga ataaaatact gatcaatcgc cccaaaccaa taccggaaat taagtaggca
     5101 atgcccgcgt tgtcagtgga aaacttttcc gccagagatg acatgttttg ggcaagcgta
     5161 ataacactaa tgccgtgcag gaagtagctg aagtaaatac aaagaacagc caggataaat
     5221 ggcgtgctga aagccttatt ttgagacatt ttcacagctc cttgctgaaa gataatttcg
     5281 ttaccgtacg tattgttctt cgacggttta cataattact atactgaaga tgattttccc
     5341 tgggtacatg gcataaagtt tatgtttcta ggaatattgc atgtcatagt ttgtttttga
     5401 tcgcgatttt ccttagttag cgcagaaaga gttatttatc cgtaagatct tgcatgacct
     5461 gaaaagaata ttgcattacc cacccggagc gacattttcc tttagcgagt gatgccgcgt
     5521 ttttttgcgg ctgcgtaagc cgataaacag tgccagtaaa aaaccgatgg cagcgatggc
     5581 ggtatcgaac cacataatat cggcaatact tctttgggac agatgagccg taatcaacgg
     5641 aatagtaaag gtcgcaatac tgcccgcact gtaatagatc cctgtagctt tacctttagc
     5701 gtaagggaaa cgttcagcca ttaacgtcag gccaatttgc acaacacctc cagcagaggt
     5761 aaaaccaatg acaaaagcaa atattatcac cacataaaat gtgggatgca ggcagacggt
     5821 aaacagagca ataaatgaga taaaggtgta cagcatcagt aatgttgttg ggcgaacggt
     5881 attacgaatg agtggagcgg taataaatac acaaagcagc gaacccacgg tgtagatact
     5941 gagtagttta atcgacatag tgtatgacat gcctgcaaca aattgtccgt actgtgccag
     6001 ccactggcta accagataaa acgttgccat tgagatatag ccatataagg tataactggc
     6061 taaatcgata attgaacagc gatgttccgt agagctggtg gttttcttta tgacaggtaa
     6121 gcgatgaccc ggatggggtg ggaacgtaca aaggtataaa aacagagcgt taataaacat
     6181 aatgcctgca gcaatcataa aggaccaacc gaaccacagt tcagcccaca ctaacaggct
     6241 aatgattagc ggtaataaaa attgtccgct ggaaacaaat gctttaatta aaatattggc
     6301 tgtcccaggt gagcgtggaa aagcttccat caaactggga taagtgcctg catcgagaaa
     6361 actgtttgcc attcctgcca gaaagccaaa aacataagcg ataatgatgt tattggtgtg
     6421 caggatgcca aaaaagaagg ccatatagca gcacatcccg agcatgataa aagggcggcg
     6481 accaaagcga tcggataata atcctgcaaa aagcaagaca cttaatcgac caatgcccag
     6541 cgatgagata actatcgaga cacccgcggc attagtctgc caaagtgtct ccagcgaggc
     6601 catattcagg ctcatcaaaa tgacgcccat accatgcacc aggtaattaa aatataaccc
     6661 aagtgcggta gggtaatagg gatttttcat aggttctact tagcaaaggt gctttaaaaa
     6721 gtgtagcgat gttgtgtcag tatccattca ttattgttgt aacaacgaca attcctttct
     6781 ctgtaaaaat taacaaagcc gctccagatc gtgtgcatat aattcagcgg ctaccgattg
     6841 atagatcttc cagtcaataa aattgccatc agggtagact cgttgaaatg cggttaagtc
     6901 ggggaaaaaa cgcatggtgt tgttgccgat gcggttattt attttctcta tgatggcatg
     6961 aagatgtttt tttctttgct gacgcatagc caacagttgc tcgactacac gatcaggaat
     7021 ggcacacttg ccattttccc actgacgcca gctttcactg tcaccggtct gggcaatcca
     7081 ggtcgcgcat tcatcaatag tcatggcaaa aatatggcgg agtgcctgga gttcataagc
     7141 gttcatcgtg taatggctgc atgaaataaa caataaaata taaataacat tatcagcctg
     7201 ctgacggctt gaagagagtc acagtatctt gtgcaacatt attggaaaga gtgaggggcg
     7261 aggtagcgaa gcggaaaaat gttaaaaaaa agcccatcgt gggagatggg caaagactac
     7321 acacagcaat tcgttgtttc actcagggga tttccatgct tataaatcaa tatgttgatt
     7381 tataaccgtg agctaatagt aggcatacgg actttttgcg tcgatcagat tcgtctcatt
     7441 agtttaaaaa ctgtaaagaa tttgcgacag gtactaccag ctgaatggcg gtaagcagga
     7501 aaatatggag aagagcaagc aaatatcaat tagcggtggt taacaatcag gcggcagagc
     7561 cgcctgatga gaaattactt attcggtttc tcgatttcgc caagttcttc gagaatttct
     7621 tccggttggt ccgtcggcgg cgggacttct tccacccacg cggcaaacag acgccaggaa
     7681 acggctaaca gaaccggacc aataaacaga ccgatcatcc cgaaagcaat caaaccacca
     7741 ataacgccag agaggatcag gatcagcggt aaatcggcac ccatgcgaat taacattggg
     7801 cggatgacgt tatccagcgt gccaaccaca ccgctccaca ctaacaatac cgttccccag
     7861 gtggtatcgc cggtccagta gagccagata atcgccggaa tcagtaccgg caacgggcca
     7921 agctggacaa ggcaggaaag gatcattaac accgttagca acgttgcata aggtacgccg
     7981 gatacggcga ggccgatacc gccaagcact gcctgtacta acgccgtcac caccacaccc
     8041 agcgccaccg cgcggatagc ctgtgccgcc agcagtacgg cggcatctcc gcgaacgcct
     8101 gccagacggg ttgcaaaatg gcgaatgcct tgtgccacct gttcaccgcg ccagtacagc
     8161 agggcactga agagaagcat cagcgcacaa tgcaccataa agcgcccgat atgcgccgct
     8221 tgcccaacga accaggtggt ggtggtgcca atataagggc ggactttcgc catgatcgca
     8281 gtccccccca tatccagcaa gttgtgccag cctgcataca gcttcgcgcc aatcaccgga
     8341 atggtattaa gccacgccag atcgggtaac gtcatgtcgc cgctggaaat ggctttaatt
     8401 agcgggccgc tgccgtcgac gatactgtta accagcaaag caatagggat gataaacacc
     8461 atcactaata acagcgtcat caccagaacg gcgagagagc ggcggccaaa catgatcttt
     8521 tgcaaacgta acaataccgg ccaggtggcg ataaccaccg taccggccca tgcaaagccg
     8581 agaataaagg gttgaacaat ccacagacat gccacaatca tgatggctaa aaacagcacc
     8641 gaaagcagaa tttgtgcgac atccctgggc tgacgtacat ttaccataac tacttttcac
     8701 cttttttgtg cgtcaaatcg tcgacgcgac gtgaacacgc gaaactcacc tgcataatga
     8761 tgagcaattt cagcggtttt taacaggccg attctgccct taattctgat gggcgtgtta
     8821 gaaaaaaatg tggtacaaca actcaggcaa cacgcaaacg atttactcgt cgtatttcaa
     8881 gttgcatgtg ctgcggctac gttcgttcac cccagtcact tactattgta agctcctgga
     8941 gttcactcgc ttgccgcctt cctgcaactc gaaatccata gagtaaataa acatcacctt
     9001 tcagtgcgct attgcagccg cctgaaagaa tagtgtatcg acctacatca cagaccgcag
     9061 gaaagggtca atataatgat tccacagatt tcccaggcac ccggcgtcgt tcaactggtg
     9121 cttaattttt tgcaagagct ggagcaacaa ggttttaccg gcgatacggc gacaagttat
     9181 gccgatcgtc tgacaatgtc gaccgacaac agtattta
//
LOCUS       DAMZET010000165         9215 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.165, whole genome shotgun sequence.
ACCESSION   DAMZET010000165 DAMZET010000000
VERSION     DAMZET010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.165"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1008
                     /locus_tag="POB34_003903"
     CDS             <1..1008
                     /locus_tag="POB34_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDI5920764.1"
                     /translation="GNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTN
                     GILLNNEWCSFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQV
                     EFNTLTVINNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIID
                     FSVPPTAYGKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLV
                     VESNGDIYECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNG
                     GCPKHRITKVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMEN
                     N"
     gene            complement(974..1096)
                     /locus_tag="POB34_003904"
     CDS             complement(974..1096)
                     /locus_tag="POB34_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920765.1"
                     /translation="MTTHPTGIYVGCFVKVIRRRLRLELRGRVINYSPFVLQHP"
     gene            1299..2984
                     /locus_tag="POB34_003905"
     CDS             1299..2984
                     /locus_tag="POB34_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HDI5920766.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
     gene            3022..5394
                     /locus_tag="POB34_003906"
     CDS             3022..5394
                     /locus_tag="POB34_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDI5920767.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTDSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGRLGMDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFANQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            5451..8234
                     /locus_tag="POB34_003907"
     CDS             5451..8234
                     /locus_tag="POB34_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDI5920768.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            8596..>9215
                     /locus_tag="POB34_003908"
     CDS             8596..>9215
                     /locus_tag="POB34_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDI5920769.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDDNVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVP"
BASE COUNT         2685 a         2024 c         2049 g         2457 t
ORIGIN      
        1 ggcaatcagg tctattttac ctggcaaggc ggtgaaccca ctctggctgg cctggatttt
       61 ttccgtaaag ttattcacta tcaacaacgc tatgcaggcc aaaaacgtat ttttaatgca
      121 ttacaaacga atggcatttt attgaataat gaatggtgtt cctttctcaa agaacatgaa
      181 tttctggttg gtatctcgat cgatggcccc caggagttac atgaccgtta cagacgcagt
      241 aattcaggta acggtacttt tgcaaaagtg atagcagcca tcgagcgtct gaaatcatat
      301 caagtagagt ttaatacgtt aaccgtcatt aataacgtta atgtccatta ccctcttgag
      361 gtttatcatt ttttaaaatc tatcggcagt aaacatatgc aatttatcga attgctagaa
      421 accgggacgc cgaatattga tttcagtggt catagtgaga acacattccg tatcattgat
      481 ttttctgtgc ctcccacggc ttatggcaag tttatgtcaa ccatttttat gcaatgggtt
      541 aaaaacgatg tgggtgaaat tttcattcgt cagtttgaaa gctttgtcag ccgttttttg
      601 gggaatgggc atacaagttg tattttccaa gagtcctgca aggataatct ggttgttgaa
      661 agtaatggag acatttacga atgcgaccat tttgtctatc cacagtacaa aattggaaac
      721 attaataaat ctgaactcaa aacgatgaac agtgtacaac tgacagcgca aaaaaaacgg
      781 atttcagcga aatgtcagca atgtgcatat aaacctatct gcaatggcgg ttgtcctaag
      841 catcgtatta ctaaagtaaa caatgagact gtttcttatt tttgcgaagg ttataaaatc
      901 cttttttcaa ccatggtacc ttatatgaac gccatggtag agttagctaa gaacagagta
      961 ccgctttatc acattatgga tgttgcaaaa caaatggaga ataattaata acacgccctc
     1021 ttagctccag tcttagcctc cgtcttatga ccttaacaaa gcacccgaca taaattcctg
     1081 tcgggtgtgt cgtcatcatc atcttccctt gcgaaaatcc tggcatacgt ggtatgcgtc
     1141 agggcattag caataccctt gattatcaat agcataacca taaccactga aaattaaaaa
     1201 tttctatata tttcatattg ttaaatgaca cttaaataaa tttcatcagt aaatcattgc
     1261 catagataat cattggatgg cacaatcaat tttcgctaat gataaccatt cccattacgt
     1321 tttgtatgtt gatagcaaag tatctctgtc tgctgaagcc cttttggctg cgtaagaata
     1381 ataaaacttc agtgttgtta atcattatta tcctcgccat gatccttggc gtggttaaaa
     1441 ttcaggtctg gttaaacgac tggaataatg attttttcaa tgcgctaagc cagaaagaaa
     1501 ccgataagct ttggcagctt gtcctttggt tccctgccct gctggggatc tttgtattaa
     1561 tatccgtaaa caaaacatgg ttaattaaac tgctgactat tcgctggcgc gaatggttaa
     1621 cagattacta ccttaatcgg tggtttgcag ataaaaacta ctacttcacg cagatctacg
     1681 gtgagcataa aaatacagat aaccctgacc aacgtatcgc tgaagacatt ctcttactga
     1741 tcagcaaaac gctcagcctt tcatttggct tcatccagtc acttagcatg ctgatcacct
     1801 ttaccgttat tctttggcaa agcgcgggta cgctctcatt cactgttggt ggaacagaat
     1861 ggaatatcca agggtatatg gtctataccg ttgtgctcat tgtgatcggt ggaactttat
     1921 ttacccataa ggtgggtaaa cgtattcgtc cgcttaatgt ggaaaaacaa cgtagcgaag
     1981 cgacatttcg aactaatctt gtgcagcata ataagcaggc agaactgatt gcgctaagca
     2041 atgctgagtc tctacaacgc caggagttgc gcgacaattt tcataccatt aaagagaact
     2101 ggcatcgttt aatgaatcgt cagcggtggc ttgattactg gcaaaatatt tattcgcgct
     2161 cgcttagcgt tcttccctac tttctgttat tgccgcagtt tattagcggg cagatcaatc
     2221 tgggcggact aatgaaatcg cgccaggcat ttatgctagt atcgaacaat ttaagctggt
     2281 ttatttataa atatgacgaa cttgctgagc tggctgcggt tatcgatcgc ttgtatgagt
     2341 tccatcaact cactgaacag cgccctacga ataagcctaa aaattgccaa catgcggtac
     2401 aagtggctaa tgcgagtatt cgtacgcctg ataataagat catattagag aacttgaact
     2461 ttcatgtttc gccaggcaaa tggctattac tgaaaggcta ctctggtgcg ggaaaaacca
     2521 cactgcttaa aacattatcc cactgctggc cgtggtttaa aggtgatatt tcttctcctg
     2581 ctgacagttg gtatgtgtca caaacaccgt taatcaaaag cggcttactg aaagagatta
     2641 tttgtaaagc acttcccctg cccgtagacg ataaatcgtt gagcgaagta ctgcatcagg
     2701 ttggtctggg gaaattggct gcgcgtattc atgaccacga tcgctgggga gatattcttt
     2761 ccagcggcga aaaacaacgt atcgccctgg cacgattaat tttacgacgt ccgaaatgga
     2821 tatttcttga cgaaactacc tctcatcttg aggaacaaga ggctatacgc ttactgcgtt
     2881 tagtgcgtga aaaactaccc acaagcggcg tcattatggt tacacatcaa cccggtgtct
     2941 ggaacctggc tgatgatatt tgtgacatta gcacggtttt ataacaacaa taaaagccct
     3001 gctgtacgga gatataaata aatgaagcga gttcttattc ctggcgtcat tttatgtggc
     3061 gctgatgtgg cgcaggccgt cgatgacaaa aacatgtaca tgcatttttt tgaagagatg
     3121 acggtctatg ctcctgtccc tgtacccgta aacggcaaca cgcattacac cagtgaaagc
     3181 atcgagcgtt taccgaccgg gaatggcaat atcagcgatc tgctgagaac caaccctgcg
     3241 gtacgcatgg attcaacgca aagtacctcg ttgaaccagg gagatattcg ccctgagaaa
     3301 atctctattc acggtgcgtc gccctaccag aatgcctatt tgattgacgg tattagtgca
     3361 actaataacc tgaacccagc gaatgagtcc gatgccagta gtgcaaccaa tattagcggg
     3421 atgtcacagg ggtattatct tgatgtcagc ttactggaca atgtgacgct ttatgacagt
     3481 tttgtgccgg ttgaatttgg tcgcttcaat ggcggggtaa ttgatgcaaa gatcaaacgc
     3541 ttcaacgctg atgatagcaa ggtgaaattg ggttatcgca ctacgcgttc ggactggtta
     3601 acatcgcata tcgatgagaa taacaagagc gcatttaatc aaggctcttc aggaagtact
     3661 tattactccc cagattttaa aaagaacttt tataccttgt cgtttaatca ggaactcgct
     3721 gataacttcg gcgttaccgc cggtttatcg cgccgccagt ctgatatcac ccgcgcggat
     3781 tatgtttcga atgacggcat tgtcgccggt cgggcacagt ataaaaacgt tatcgatact
     3841 gcattgagca aatttacctg gtttgccagc gaccgcttta cccacgattt aaccttaaaa
     3901 tataccgact ccagccgtga ttataatacc agcaccttcc cgcagtctga tcgcgaaatg
     3961 ggtaataaat cctatggtct ggcatgggat atggatacgc agctcgcatg ggccaaactg
     4021 cgtaccaccg ttggttggga tcatattagt gattataccc gtcacgatca tgacatctgg
     4081 tacaccgaac tttcatgtac atatggtgat attacagggc gttgcacccg tggcggatta
     4141 ggacacattt cccaggctgt agataattac accttcaaaa cacgcctgga ctggcaaaaa
     4201 ttcgccgtgg gtaatgtttc gcatcaaccc tacttcggcg cggaatacat ctattccgat
     4261 gcgtggactg aacgccataa ccagtctgaa tcctatgtga ttaatgctgc cggaaagaaa
     4321 actaaccata ccatttacca taaaggtaaa ggccgcctgg gaatggacaa ctacacgctg
     4381 tatatggcgg atcgcattag ctggcgtaat gtgtcattaa tgcccggcgt gcggtatgac
     4441 tatgacaact atctgtcaaa ccacaatatc tccccgcgct ttatgacgga atgggatatt
     4501 tttgctaatc aaacctcaat gattacagca ggttataacc gttactatgg cgggaatatt
     4561 cttgatatgg gattacgtga tatccgcaat agctggacgg aatcggtatc aggtaataaa
     4621 actctgacgc gttatcagga tttgaaaacg ccttataacg atgaactggc aatgggattg
     4681 cagcagaaaa tcggtaagaa cgttattgcg cgtgcaaact atgtttaccg tgaagcgcat
     4741 gatcaaatca gcaaaagcag tcgtaccgac agcgcgacta aaaccaccat tactgaatat
     4801 aacaacgatg gcaaaaccaa aacgcattcg ttcagcctca gttttgaact ggccgaaccc
     4861 ctgcatatcc gccaggtaga tattaaccca caaattgtct ttagctatat caagagcaag
     4921 ggcaacttgt cgttaaacaa tggttatgag gagagcaata ccggtgataa ccaggtggtt
     4981 tataacggta atctggtctc ttacgatagc gttccagtgg cagattttaa taacccatta
     5041 aagatctcct taaacatgga tttcacgcat caaccgagcg ggttagtgtg ggcgaatacg
     5101 ctggcctggc aagaagcgcg taaagctcgc attatccttg gtaagacaaa tgcgcaatac
     5161 atcagcgaat attcagatta caagcagtat gttgacgaaa aactggatag cagcctgacc
     5221 tgggacaccc gcttgtcctg gacgccacaa tttctgaaac aacaaaacct gacgatcagt
     5281 gccgatattc tcaatgtact ggatagcaaa accgctgttg atacaacgaa caccggtgtg
     5341 gcgacctacg ccagtggccg tactttctgg cttgatgtta gcatgaaatt ttaatagttc
     5401 ggattttact taaccgggta aattcgcccg gttttcgcat ggagataatt atgagaaacc
     5461 tctgtttctt actgacgtta gtggcaactc tgttgctccc cgggcggctg attgccgccg
     5521 ccttaccgca ggatgaaaag ttaattaccg ggcaactgga caatggcttg cgatatatga
     5581 tttatccgca tgctcatcca aaggatcagg taaatttatg gctgcaaatt cataccggtt
     5641 cattgcagga agaagacaat gagcgcggcg tggctcattt tgtagaacat atgatgttta
     5701 acggcacaaa aacatggccg ggtaataaag tcatcgaaac atttgagtca atgggcctgc
     5761 gttttggtcg cgatgttaat gcctatacca gctatgacga aacggtgtat caggtgagtt
     5821 tgccgactac gcagaaacaa aatctgcaac aagtgatggc aatcttcagt gaatggagta
     5881 atgccgcaac ctttgaaaaa ctcgaagtag acgctgaacg tggcgtaatt actgaggaat
     5941 ggcgtgccca tcaggatgcg aaatggcgca cctctcaggc gcgccgccct ttcctgctgg
     6001 caaatacccg taatttagac cgtgaaccta tcggcctgat ggatactgtc gccacggtca
     6061 caccggcaca attgcgccaa ttttatcaac gctggtatca accaaataat atgaccttta
     6121 tcgtggtcgg cgatatcgac agtaaagaag cgctggcgct gataaaggat aatttaagta
     6181 agcttccggc taacaaagca gctgaaaatc gcgtctggcc gacaaaagcc gaaaaccacc
     6241 tgcgctttaa tatcattaat gataaagaaa accgggtgaa cggcatcgca ctctattatc
     6301 gcctgccaat ggtacaagtg aacgatgagc aaagctttgt cgaacaagct gaatggagca
     6361 tgttagttca gctgttcaat caacgtctgc aggaacgcat acagtcgggc gagttgaaga
     6421 ctatttctgg cggcactgcg cgcagcgtta aaattgcacc cgattatcag tcgctgtttt
     6481 tccgtgtaaa tgcacgagac gataatatgc aggatgctgc gaatgcatta atggcagagt
     6541 tggcaaccat tgatcagcat ggtttttctg ctgaagaact cgatgatgtc aaatctaccc
     6601 gcctcacctg gctgaaaaat gcggttgatc agcaagctga acgtgattta cgtatgctga
     6661 ccagtcgcct ggcatccagc tcattaaata atacgccgtt cttgtcgccg gaagagacat
     6721 atcaactttc gaaacgtctg tggcagcaaa ttaccgtgca aagtctggcg gaaaaatggc
     6781 agcagttaag aaagaaccag gacgcatttt gggagcaaat ggtaaacaat gagcttgccg
     6841 ccaaaaaagc attgtctcct gcggctatcc tggcgctgga aaaagagtac gccaacaaaa
     6901 agctggcggc ttacatcttc ccaggcagaa atttatcgtt aacagtagac gctgacccac
     6961 aggcggaaat tagcagcaaa gaaacgctgg ctgagaatct gacatcatta acactttcca
     7021 atggtgccag ggttattctg gcaaaatccg cgggtgaaga gcaaaagcta caaattactg
     7081 ccgtatcgaa taaaggcgat ttaagtttcc ctgcgcagca aaaatcactt atcgcgctgg
     7141 caaataaagc agtaagcgga agcggcgttg gtgagctctc ctcttccagc ctgaaacgct
     7201 ggagtgcgga aaattcggta accatgagca gtaaagtcag tggcatgaat acgttgctct
     7261 ccgttagcgc gcggactaat aaccctgaac ctggttttca gttgattaac cagcgaatca
     7321 cccacagcac gattaacgac aatatttggg catcgctaca aaatgctcaa attcaggcgt
     7381 tgaaaacgct cgaccagcgt ccagcggaga aattcgccca gcagatgtat gagacgcgct
     7441 atgctgatga ccgcacgaaa ttactgcaag aaaatcagat tgtacagttt actgccgcag
     7501 atgcgctggc tgccgatcgc cagttgtttt catctccagc ggatatcacg tttgtcattg
     7561 tcggtaatgt ctcagaagac aaactcgtgg cgttaattac gcgttactta ggatcaatca
     7621 aacactctga ttcgccatta gccgcaggta aaccattaac tcgcgcgacg gacaacgcat
     7681 cggttactgt aaaagaacaa aatgaacctg tggcacaggt ttcacagtgg aagcgttatg
     7741 attcccggac acctgttaat ctggcgacgc gtatggcgct cgatgctttt aacgtcgcac
     7801 tggcaaaaga tctacgtgtt aatattcgtg aacaggcatc tggagcatac agcgtttctt
     7861 ctcgcctctc ggttgatcct caggccaaag atatcagtca tttgctggct tttacttgtc
     7921 aaccagaacg acatgatgaa ctgttaacgt tagcgaatga agtgatggtt aagcgtctgg
     7981 ctaaagggat cagtgagcaa gaactgaatg aataccagca aaacgttcag cgcagcctcg
     8041 atatccaaca gcgtagcgtt caacaattag cgaacactat tgtaaatagt cttattcaat
     8101 atgacgatcc tgcagcatgg actgagcagg agcaattgtt gaaacaaatg acggtagaga
     8161 atgttaacac tgccgttaaa caatatcttt ctcatccggt aaatacttat accggagtat
     8221 tattgccaaa ataataacag ccccgtcaac acatcgttgg cggggatttt agcaatattc
     8281 gctattttta tgtaataatt ttataaatgc gttcaaaata ataatcaagt actaatagtg
     8341 atattttaag gtctgatttt tacgtgataa ttcaggagac acagaatgcg cataaaaata
     8401 acagcataaa acaccttacc accacccaag aatttcatat tgcattgttt ttcaatgaaa
     8461 aaatattatt cgcgtaatat ctcacgataa ataacattag gattttgtta tttaaacacg
     8521 agtcctttgc acttgcttac tttatcgata aatcctactt ttttaatgcg atccaatcat
     8581 tttaaggagt ttaaaatgga taagaagcaa gtaacggatt taaggtcgga actactcgat
     8641 tcacgttttg gtgcgaagtc tatttccact atcgcagaat caaaacgttt tccgctgcac
     8701 gaaatgcgcg acgatgtcgc attccagatt atcaatgacg aattatatct tgatggcaat
     8761 gctcgtcaga acctggccac tttctgccag acctgggacg acgacaatgt ccacaagttg
     8821 atggatttat ccattaacaa aaactggatc gacaaagaag aatatccgca atccgcagcc
     8881 atcgacctgc gttgcgtaaa tatggttgcc gatctgtggc atgcgcctgc gccgaaaaat
     8941 ggtcaggccg ttggcaccaa caccattggt tcttccgagg cctgtatgct cggcgggatg
     9001 gcgatgaaat ggcgttggcg caagcgtatg gaagctgcag gcaaaccaac ggataaacca
     9061 aacctggtgt gcggtccggt acaaatctgc tggcataaat tcgcccgcta ctgggatgtg
     9121 gagctgcgtg agatccctat gcgccccggt cagttgttta tggacccgaa acgcatgatt
     9181 gaagcctgtg acgaaaacac catcggcgtg gtgcc
//
LOCUS       DAMZET010000166         9021 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.166, whole genome shotgun sequence.
ACCESSION   DAMZET010000166 DAMZET010000000
VERSION     DAMZET010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.166"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..281
                     /locus_tag="POB34_003909"
     CDS             <1..281
                     /locus_tag="POB34_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDI5920770.1"
                     /translation="ISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDA
                     VGISGDDMEALAFAWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            379..708
                     /gene="mliC"
                     /locus_tag="POB34_003910"
     CDS             379..708
                     /gene="mliC"
                     /locus_tag="POB34_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HDI5920771.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            767..1423
                     /gene="pdxH"
                     /locus_tag="POB34_003911"
     CDS             767..1423
                     /gene="pdxH"
                     /locus_tag="POB34_003911"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HDI5920772.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1552..2826
                     /gene="tyrS"
                     /locus_tag="POB34_003912"
     CDS             1552..2826
                     /gene="tyrS"
                     /locus_tag="POB34_003912"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDI5920773.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2885..3748
                     /gene="pdxY"
                     /locus_tag="POB34_003913"
     CDS             2885..3748
                     /gene="pdxY"
                     /locus_tag="POB34_003913"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HDI5920774.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3792..4397)
                     /gene="gstA"
                     /locus_tag="POB34_003914"
     CDS             complement(3792..4397)
                     /gene="gstA"
                     /locus_tag="POB34_003914"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HDI5920775.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGFEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4502..6004)
                     /gene="dtpA"
                     /locus_tag="POB34_003915"
     CDS             complement(4502..6004)
                     /gene="dtpA"
                     /locus_tag="POB34_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HDI5920776.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(6616..7251)
                     /gene="nth"
                     /locus_tag="POB34_003916"
     CDS             complement(6616..7251)
                     /gene="nth"
                     /locus_tag="POB34_003916"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDI5920777.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(7251..7946)
                     /gene="rsxE"
                     /locus_tag="POB34_003917"
     CDS             complement(7251..7946)
                     /gene="rsxE"
                     /locus_tag="POB34_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HDI5920778.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7950..8570)
                     /gene="rsxG"
                     /locus_tag="POB34_003918"
     CDS             complement(7950..8570)
                     /gene="rsxG"
                     /locus_tag="POB34_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HDI5920779.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8574..>9021)
                     /locus_tag="POB34_003919"
     CDS             complement(8574..>9021)
                     /locus_tag="POB34_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HDI5920780.1"
                     /translation="IMQYPIYSGILAGAGWQWVNLAWLAGGLWLLWQKAIRWHIPLSF
                     LVTLALCATLGWLFSPETLAAPQIHLLSGATMLGAFFILTDPVTASTTNRGRLIFGAL
                     AGLLVWLIRSFGGYPDGVAFAVLLANITVPLIDYYTRPRVYGHRKG"
BASE COUNT         2295 a         2249 c         2311 g         2166 t
ORIGIN      
        1 ccatttctga acaagttttg ttgagcggtg gctgcgaacg attgatggta tgtggtggag
       61 gtagtcgtaa tccgctactc atggcgcgtc tggcggcatt actgccaggc acagaagtca
      121 ccaccaccga tgccgttggc attagtggcg atgacatgga agcattggct ttcgcctggc
      181 ttgcctggcg gacgctggcg ggattaccag gaaatctgcc ttccgtcact ggcgcaagcc
      241 aggagacggt actgggggct attttccccg ctaacccgtg atatcagagt taactgaatt
      301 tttcttaccg cgtctgccgt tacacttgga acgttaggga gggcgtattg ccctccagac
      361 caggaaagtc ttcgggatat gactatgaaa aaactgttaa ttatcatttt gccggtcctt
      421 ctctccggct gtagcgcctt taatcagctg gttgagcgca tgcaaaccga tacgctggaa
      481 taccagtgtg atgaaaaacc gttgacggtc aaactgaata atccgcgcca ggaggtcagt
      541 tttgtttacg ataatcaact actgcatctc aaacagggca tttcagcctc tggcgcgcgt
      601 tacactgacg gaatctatgt tttctggtcg aaaggcgatg aagcgactgt ctataaacgc
      661 gaccgcatcg tcttgaataa ctgtcagtta caaaatccac agcgttgaga tttttccagg
      721 ggcggcgcac aatagcgtca cccactgaca atccgtaaag aaaaccatgt ctgataacga
      781 cgaattgcag caaatcgcgc atctgcgccg tgaatacacc aaaggcgggt tacgccgccg
      841 cgatcttccc gccgatccat taaccctttt tgaacgttgg ctctctcagg cttgtgaagc
      901 caaactggcg gaccctaccg cgatggtggt cgctaccgtg gatgaacatg gtcagcctta
      961 tcagcgcatc gttttactca aacattacga cgaaaaaggc atggtgtttt acaccaacct
     1021 cggcagccgt aaagcacatc aaatcgaaaa taatccgcgc gttagcctgc tgttcccgtg
     1081 gcataccctt gagcgccagg tgatggtgat cggtaaagca gaacgacttt cgactctcga
     1141 agtgatgaaa tattttcata gccgcccgcg tgatagccag attggtgcat gggtttcgaa
     1201 gcagtccagt cgcatttctg cccgcggtat ccttgaaagt aaattcctgg agctgaagca
     1261 gaagtttcaa cagggcgaag tgccattgcc gagcttttgg ggcggttttc gcgtcagcct
     1321 tgaacagatt gagttctggc agggtggtga gcatcgcctg catgaccgct ttttgtacca
     1381 gcgtgaaaat gatgcgtgga agattgatcg tcttgcaccc tgaaaagatg caaaaatctt
     1441 gctttaatcg ctggtactcc tgattctggc actttattct atgtctcttt cgcatctggc
     1501 gaaaagtcgt gtaccggcaa aggtgcagtc gttatataca tggagatttt gatggcaagc
     1561 agtaacttga ttaaacaatt gcaagagcgg gggctggtag cccaggtgac ggacgaggaa
     1621 gcgttagcag agcgactggc gcaaggcccg atcgcgctct attgcggctt cgatcctacc
     1681 gctgacagct tgcatttggg gcatcttgtt ccattgttat gcctgaaacg cttccagcag
     1741 gcgggccaca agccggttgc gctggtaggc ggcgcgacgg gtctgattgg cgacccgagc
     1801 ttcaaagctg ccgagcgtaa gctgaacact gaagaaactg ttcaggagtg ggtggacaaa
     1861 atccgtaagc aggttgcccc gttcctcgat ttcgactgtg gagaaaactc tgctatcgcg
     1921 gcgaacaact atgactggtt cggcaatatg aatgtgctga ccttcctgcg cgatattggc
     1981 aaacacttct ccgttaacca gatgatcaac aaagaagcgg ttaagcagcg tctcaaccgt
     2041 gaagatcagg ggatttcgtt cactgagttt tcctacaacc tgttgcaggg ttatgacttc
     2101 gcctgtctga acaaacagta cggtgtggtg ctgcaaattg gtggttctga tcagtggggt
     2161 aacatcactt ctggtatcga cctgacccgt cgtctgcatc agaatcaggt gtttggcctg
     2221 accgttccgc tgatcaccaa agcagatggc accaaatttg gtaaaactga aggcggcgca
     2281 gtctggttgg atccgaagaa aaccagcccg tacaaattct accagttctg gatcaacact
     2341 gcggatgccg acgtttaccg cttcctgaag ttcttcacct ttatgagcat tgaagagatc
     2401 aacgccctgg aagaagaaga taaaaacagc ggtaaagcac cgcgcgccca gtatgtactg
     2461 gcggagcagg tgacccgtct ggttcacggt gaagaaggtt tgcaggcggc aaaacgtatt
     2521 accgaatgcc tgttcagcgg ttccttgagt gcgctgagtg aagcggactt cgaacagctg
     2581 gcgcaggacg gcgtaccgat ggttgagatg gaaaagggcg cagacctgat gcaggcactg
     2641 gtcgattctg aactgcaacc ttcccgtggt caggcacgta aaactatcgc ctccaatgcc
     2701 atcaccatta acggtgaaaa acagtccgat cctgaatact tctttaaaga agaagatcgt
     2761 ctgtttggtc gttttacctt actgcgtcgc ggtaaaaaga attactgtct gatttgctgg
     2821 aaataatgca ttaagtggaa agggggagtg agaaatcact ccccctggtt tttatacagg
     2881 gaacatgatg aaaaatattc tcgctatcca gtctcacgtt gtttatggtc atgcgggtaa
     2941 cagtgcggca gagtttccga tgcgccgcct gggcgcgaac gtctggccgc tgaacaccgt
     3001 tcaattttct aatcacaccc aatacggcaa atggactggc tgcgtgatgc cgcccagcca
     3061 tttaaccgaa attgtgcaag gcattgccgc cattgataaa ttacacacct gtgatgccgt
     3121 attaagtggc tatctgggat cggcggagca gggtgaacat atcctcggta tcgtccgtca
     3181 ggtgaaagcc gcgaatccgc aggcgaaata tttttgcgat ccggtaatgg gtcatccgga
     3241 aaaaggctgt atcgttgcac cgggtgtcgc agagtttcat gtgcggcacg gtttgcctgc
     3301 cagcgatatc attgcgccaa atctggttga gctggaaata ctctgtgagc atgcggtaaa
     3361 taacgtcgaa gaagcggttc tggcagcgcg cgaactcatt gcgcaagggc cacaaattgt
     3421 gttggttaaa cacctggcgc gagctggcta cagccgtgac cgttttgaaa tgctgctggt
     3481 caccgccgat gaagcctggc atatcagccg tccgctggtg gattttggta tgcgccagcc
     3541 ggtaggtgtt ggtgatgtga cgagcggttt actgctggtg aaactgcttc agggggcaac
     3601 gctgcaggag gcgctggaac atgtgaccgc tgcagtctac gaaatcatgg tgaccaccaa
     3661 agcaatgcag gaatatgagc tgcaagtggt ggctgctcag gatcgtattg ccaaaccaga
     3721 acattacttc agcgcaacaa agctctgaaa tatctcattt cggcccacaa cgacctgtgg
     3781 gccgatggct attactttaa gccttccgct gacagcgcgt cttgtacttc cggacgttca
     3841 gccatacgtt gcataaatgc tgcaatgtgc tcaaaccctt ccagattcag tttcaccgcg
     3901 tatgcccagc gcagaaccgt aaacagatag gcatcagcaa ttgtaaatct ttgcccgcag
     3961 atccagtgct catccttcag tgcctcgttc acatattgca gcttcttctc cagctgcgcg
     4021 cgaactgtcg gtttgtactc ttccggtgta tcagggcgaa acagaggtgt gaaaccttta
     4081 tgcagctcgg tggcgatgta attcagccat tcgatggttt tatagcggga aatactgttt
     4141 accggtgcca gcaactggcg gtcggggacg ctgtcggcaa gatactgcat aatcgctacg
     4201 ccttccgtca gcaaagtacc gtcatccagc agcaatgcag gcacctgccc cttagggtta
     4261 acggcaaagt aatcgtcacc gttttcgaga cgttttttca ttaaatccac actgacgagg
     4321 gtaaaatcct ttccgctctc acgcagggtg atatgggaag cgagagagca ggcacccggt
     4381 ttgtagaaca atttcatcgg taactccttt tgtctgtggt ttaaagtatg gtagtgcgtt
     4441 gctgaacaaa aaaaagccgc taacgaaaag ttagcggctt gtaaaagagt ttccctgaat
     4501 tttacgctac ggctgctttc gccgctttgt ctgcagcgtc atcctgcgtc atgcggtgca
     4561 gtttcggcgc ggtcagcagc atcagtactg caataacggc agtagcgaca ccaatctgca
     4621 agaatacgcg accatagact tccagtgaca tcagcggatc ggtaacgtta tccggcacag
     4681 ccatcatacc cgcaacataa ccaccaatca ggtttgcacc ggcagtggtc aggaaccagc
     4741 tacccataat gaagcccatc agacgctgcg gaacgagttg agcaaccatt gccagaccca
     4801 gaccagagat catcagttcc ccgatgctct gcaggccata gcttgcgacc agccagctta
     4861 cagacacgat accagcgtca gacgcgaatt tcgcacccag cggcagaatc aggaacgcac
     4921 cagaacacat caccatgccg attgcaaact tggttggcat cggcagggta tcgcccatct
     4981 tgttatagat agcggccaga atcggactac cgatgatgat ccagaacggg ttcagtgcct
     5041 gatactgctc aggttctacg gccagaccca gaatggagtg ctcaacgtta cgaatcgcaa
     5101 agaagttcag tgacgttggc atctggctgt acagcacgaa gaagataatg gcttcgagca
     5161 tcaggatgaa ggcaacgatc attttacgac gcgcagcacc tttcatcgcg aaggcttctt
     5221 taccgaagat aaccacgata ccgaaggcaa caacgcccag cgccatacgc gcaacttcct
     5281 gattgtgcag cagccaggtg gcgatagcga tcagtgccac aacaccaata atggtcagca
     5341 gcaggttacg gtagttgata ggctcgaagt ctggttttga accgtactgt ttaacccagc
     5401 gttggcagaa ggcgaagtta acgatagtga tcagcaggcc tacaacgctc aacgcaaacg
     5461 caacactcca gccgtatttc gcggccagcc acggcgtagc aatcatagag aagaaagagc
     5521 cgatgttgac ggacatgtag tacatggtga atgcaccgtc cagacgcggg tcgtttttct
     5581 catagcatgt agaaagcaga gaagacgggt tagctttaaa caggccgtta ccgaccgcaa
     5641 tagccgccat acccatataa acgataccgg cgtcgtgacc agaccaggca accagagcat
     5701 aaccaatcgc cagcacaata gcgccgagca taattacgcg tttagtaccc agtaccttgt
     5761 cacctaacca gccgccgata gcgaccagac cataaaccag ggcactaaag gaagagaaaa
     5821 gggtgattga atccgcttca gacataccca gttgtttaac caggtaaaca gccataattc
     5881 cttgtaggcc gtaataacca aaacgttccc ataactcaat cgagaagatg agatagaacg
     5941 ccttcggttg tttgaaagcg ttcaaactga cgctttcagt tggtttttgg tttgcagtgg
     6001 acacttttac ctcttttttt acatcccata ttaacggggg tgctttctcg cgttatgtat
     6061 aaaaaacata cgcgcaattg ttatagtggg aggggaaacg gcaggtaatg ttcactatct
     6121 tgcactttca gacaagtcct ttgtaataat ctgttacata taactgaggc ggggaattca
     6181 accattccgg gaaatgttat ccagcgttaa atttttcaaa aaacgtaacg gcagattttt
     6241 tcactgcact gagagggggg gtgatggctt aacggcgata tgttctgcta tttagcgttt
     6301 ttactagtga gatagtccag tttctgaaaa atagccagtg taatgttttg taggtcaata
     6361 tttgttgcgt gtgtaaattt aaatgatttt aacctgattc ttgcaatgtt tttacattgc
     6421 gacagcatcg tgatcttgat cacgaatgaa tagaaaattt cgctattaaa aaaacaatta
     6481 acctgcattt ttggttatcc gcaaacggat tacctgatga taacgctaag caatggcatc
     6541 attgacgtta aaaagatgtt ttaaaagagg ataaagaaag gttatcaatg gggtaatcgg
     6601 tgttacccct tttcttcaga tgtcaacttt ctctttgtat tcacaaagat cttcaataat
     6661 acaagagcca cagcggggct tgcgggcaat gcaggtataa cgcccgtgca ggatcaacca
     6721 atggtggcag tcgactttaa actctgccgg aaccactttc agtagctttt cttctacctg
     6781 ttcgacgttt ttccccggcg caaattgagt acgattacaa acgcggaaaa tgtgcgtgtc
     6841 gacagcaata gtcggccagc cgaatgcagt gtttaatacg acgttggctg ttttacgacc
     6901 tacgccgggc agggcttcaa gcgcagcacg atcttccgga acctcgccat tatgctgctc
     6961 cagcaagata cggcaggttt tgatgatatt ttctgctttg ctgttataaa gcccaatcgt
     7021 tttgatatag gttttcaccc cttcaacgcc cagttcaagc atcgctgcag gcgtattcgc
     7081 caccgggtag agtttcgccg tcgccttatt aacactgaca tcggtcgcct gagcggaaag
     7141 cagtacggca atcagcaatt caaaaggcga actgaaatta agctcggtgg tgggatgagg
     7201 attgttctca cgcaggcgag tgaggatctc caggcgtttt gctttgttca tcagacattc
     7261 cctgtttcac cgtttggcaa tgcacgttcg gcagctgctt cagcacggcg ctttttcatt
     7321 ctttcatcaa tcaggtattt tcctgccagc atcagtccca ggccaataaa tgcacctgga
     7381 ggcagcatcg ccagcaggaa aggggagtcg gtgtggaaaa tttccacgcg taatactttt
     7441 gcccagctac ctaacagcgc atctgcaccg tcaaacaatg tgccattgcc gataatttcg
     7501 cgtagtgaac ccagcacgaa catggcgcag gttgcgccca taccaattga aaagccgtcc
     7561 agtgccgaaa gcgccggacc ttttttggcg gcgaaggctt cagcgcggcc cacaacgata
     7621 cagttagtga caatcagcgg aataaaaatc cctaatgatt gatataggcc aaaggcgtag
     7681 gcgttgatca gcatctgtac agcgctgacc accgaggcga tgatcatcac gtaaatggga
     7741 atgcggatct cggctggcgt ccagtgacgc agcgtcgaaa tggtcaggtt ggtcagcgtc
     7801 agtaccagcg tagtcgcaag tcctaaaccc agagcgttag tggcagtgga cgtgaccgcc
     7861 aacagaggac aaaggccgag caactggacc agagcagagt tgtttttcca caacccctga
     7921 acaataacgt ctttaatttc gctcacgctt tattctccac aggcaggaag ttgagaaagt
     7981 tgtgccggta acgtctgagc gtacaatccg gcgcgtttta ccgcattaac caccgcgcgg
     8041 ggagtaatcg tcgcgccggt gaactggtcg aaatcaccac catctttctt caccgcccag
     8101 tgcgcatcat ccgcaccact gattttttta cccgcaaaat gggtgatcca gtcagaaagg
     8161 cgcagttcga ttttatcgcc aagccctggc gtttcgtggt gctctgtcac gcgcgtgcca
     8221 agtaccgtgc cgttaaaatc ggctcccacc agcagctgaa tcgcaccgga atagccatct
     8281 ggcgcggttg cttccagaac ggcggctacc ggtttgtcat cctgtttggc gatgtaaacc
     8341 cgatgctcac ctttacctaa ctctggcgca gttaccagat agcaactctg tgccagcgca
     8401 ttgttatagc gttcggctgg cagcacctga tcaaataatg ccttttgttg cagactggcc
     8461 tgttcagcaa tcgtcgtttt ggtcatctgg ttgatggccg cagttaaccc tgttgaaccc
     8521 gctgcaaata gcgctaacgt aatgccgtgt tttcggatag ttttcagcat ggtttaccct
     8581 ttgcgatggc cgtagacgcg cggacgcgtg tagtaatcga tcagaggaac cgtgatgttc
     8641 gccagcagga cggcaaaagc cacgccgtca ggatagccgc cgaaactgcg gatcaaccag
     8701 actaataagc ccgccagcgc gccgaaaata agacgaccac gattggtcgt agaagcggta
     8761 accgggtcag tcaaaataaa gaatgcgccg agcatggtcg caccagacag cagatgaatt
     8821 tgcggtgctg ccagtgtttc tggtgagaac aaccagccca acgttgcgca taacgccagc
     8881 gttactaaga agctgagggg aatatgccag cgaatcgctt tctgccatag caaccacagg
     8941 ccgccagcca gccaggcgag atttacccat tgccaaccag cgcccgccag aataccgctg
     9001 tagatcggat attgcataat c
//
LOCUS       DAMZET010000167         9005 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.167, whole genome shotgun sequence.
ACCESSION   DAMZET010000167 DAMZET010000000
VERSION     DAMZET010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.167"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..133)
                     /locus_tag="POB34_003920"
     CDS             complement(<1..133)
                     /locus_tag="POB34_003920"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HDI5920781.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     "
     gene            420..1178
                     /locus_tag="POB34_003921"
     CDS             420..1178
                     /locus_tag="POB34_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HDI5920782.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(1231..1338)
                     /locus_tag="POB34_003922"
     CDS             complement(1231..1338)
                     /locus_tag="POB34_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920783.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1514..3383)
                     /gene="espL1"
                     /locus_tag="POB34_003923"
                     /pseudo
     CDS             complement(1514..3383)
                     /gene="espL1"
                     /locus_tag="POB34_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            3907..5835
                     /gene="thrS"
                     /locus_tag="POB34_003924"
     CDS             3907..5835
                     /gene="thrS"
                     /locus_tag="POB34_003924"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDI5920784.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITTKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            5839..6381
                     /gene="infC"
                     /locus_tag="POB34_003925"
     CDS             5839..6381
                     /gene="infC"
                     /locus_tag="POB34_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDI5920785.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            6478..6675
                     /gene="rpmI"
                     /locus_tag="POB34_003926"
     CDS             6478..6675
                     /gene="rpmI"
                     /locus_tag="POB34_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDI5920786.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            6728..7084
                     /gene="rplT"
                     /locus_tag="POB34_003927"
     CDS             6728..7084
                     /gene="rplT"
                     /locus_tag="POB34_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDI5920787.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    7201..7325
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            7207..7251
                     /gene="pheM"
                     /locus_tag="POB34_003928"
     CDS             7207..7251
                     /gene="pheM"
                     /locus_tag="POB34_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HDI5920788.1"
                     /translation="MNAAIFRFFFYFST"
     gene            7535..8518
                     /gene="pheS"
                     /locus_tag="POB34_003929"
     CDS             7535..8518
                     /gene="pheS"
                     /locus_tag="POB34_003929"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDI5920789.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            8533..>9005
                     /gene="pheT"
                     /locus_tag="POB34_003930"
     CDS             8533..>9005
                     /gene="pheT"
                     /locus_tag="POB34_003930"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="catalyzes a two-step reaction, first charging a
                     phenylalanine molecule by linking its carboxyl group to
                     the alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; forms a tetramer of
                     alpha(2)beta(2); binds two magnesium ions per tetramer;
                     type 2 subfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDI5920790.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNT"
BASE COUNT         2443 a         1968 c         2203 g         2391 t
ORIGIN      
        1 cgacgttgat gccgccgccc ccgggttcga acaccggtgc ggtacagcgc agttttcctt
       61 cgggataaat ttgcggggta attgttgcgc tatcgagaga gggcgcaagt gtcaacgtat
      121 agatacgtac catcatttcc tcctataggc tgatttcagt ctggcaccga atcagccgaa
      181 agtgaagaaa ttaacaatat gatttattag agctttattt aaaattttgc agataaatat
      241 atataaataa aaatctgttt tcgccacatg cttattcaaa aaatgaaata agaatacatt
      301 gctaagttat tcgcgccttt ttataattcg ttacctttcc agggacgcta atctttgatc
      361 cttaagaaag tttccgggac cgcgatggtc tctttttttg ttgtacggac tcttgataaa
      421 tgaagctttt gaagacagtt cctgccatcg ttatgttggc ggggggcatg tttgcgtcac
      481 tgaatgcagc tgccgatgat tccgttttta ctgtcatgga cgatcctgcc agcgctaaaa
      541 aacctttcga aggtaatctg aacgcaggat atcttgcaca atcaggcaat acgaaaagct
      601 cctcacttac ggctgacacc accatgacct ggtatggaca aaccactgcc tggtcgctgt
      661 gggggaacgc cagtaatact tcttctaatg atgagcgttc ttcagaaaaa tatgcggcgg
      721 gcgggcgtag ccgttttaat ttaactgatt atgactattt atttggtcag gcaagctggc
      781 tgacagaccg ttataacggc tatcgagagc gcgatgtgtt gaccgcgggt tatggtcgtc
      841 agtttttgaa tggcccggta cacagtttcc gctttgaatt cggtccaggt gtgcgttacg
      901 acaaatatac cgacaatgcc agtgaaactc agccgctggg ctatgcctcc ggtgcttatg
      961 catggcagtt gactgacaac gctaaattta cgcagggtgt ttccgtcttt ggcgcggaag
     1021 acacaacgct gaactccgaa agcgcattaa atgttgctat taatgaacat tttggactga
     1081 aagtggctta caacgtcaca tggaactctg aacctccaga gtctgcaccg gaacataccg
     1141 atcgtcggac aaccttatcg ctgggctaca gcatgtaaaa ttatctgccg gaccgtgtta
     1201 tgcggtctgg ctgtcttcgt ttgtcactgc tcagccccaa atcattgctg cccaacgtag
     1261 caacaacata gcgccacaga ttgccagtag caccaccacc atcttccagt gttttttatc
     1321 aaagcgttta gtcggcatac cagtgtcctg catatctctt gtgcgtgata acggagtgta
     1381 acaaaaggga caggcatcaa gggatttttt gttattacag ccgtcgggtg gcgttgtaca
     1441 cctgatcact gtttctgcgt tttatgaaaa aaataaaatt atgtatcgat atacaaatat
     1501 cataaatagc tggttataaa tacgttttta gttcatcagc actaaaactc aggctttggc
     1561 tgattgcatt aatagcagca taatatgttt ctacagtttt ataatgatta tgatgaatgg
     1621 ctatatgaac agctgacatg ttttcatgat ttttagcggc caacagtgtg aataattgac
     1681 gttgactaaa agaatatttt tttgcaaaag tactcagtgt tgatatataa gataacacga
     1741 cgtctttatt acccttgctc atggcgatgt ataaagcagg ggttccatgt gctagctttg
     1801 ctcaacttgt atttaaaggc gatgccatta attttagaga ggaaccgtgt gacacacaaa
     1861 gggtgattat tttccattgc ggcgtataat cctggctcac catcatgtat tttcatggct
     1921 aaaatactca tcaccagttt tttgtttaaa agctcttgtg ctattggagt ggttaatgca
     1981 ttcagaaaga tatcgattat ttcatcgaat ccatacaaca tggagatata aagtccaggt
     2041 gtttcgttag cacttttagt ttgcagtaat ttaaccagat tatcttcatg aatgatgtga
     2101 ttatctacca gtgactgaat ttcttgcata aatattttca cgttgtcagt atgtccgttc
     2161 atcatagcca aaaataaagc aggtgtacca acgctattct gcgcagttat tatttcaatg
     2221 atttcttttt cagggagtat gggcaactgt ttgaataatg taataagctg ttgagtctgg
     2281 cctgtagcca ttattaacaa cattgccgat ggatgtatag gggcaaatgg cattcgtgat
     2341 gcaatacccg cgaaaatagc ccctggaact cccacaggta ataggggcag aatagcaatt
     2401 gcacagttgc gaacatacct atcagcccag gtctgagcat cgggacctga actgatgaac
     2461 ttatcaagcg aaagaaaggc ccgtgctgtc ctgtgggtgt ctctgacggc aacgttggta
     2521 tcgttcgggt cgtaaacgga cacaacatag tttaattgtc catacactga ctgctttatt
     2581 cgtagcctta ctgacaaagt atgattgtct atatataaaa tcgcggacat atgactgaca
     2641 ttgtttgcca tcattcgaca aaagaaaata tctaaagcga agccaaaatc cttactcgcg
     2701 ataacgcgac tccctgcagg aggatggcga agtaatgctc tgaactccgc atcccggact
     2761 aaggggatat gctgctgtat ggattcaacg gaggagtaat gtttaatcag gttgactttg
     2821 ccttttccat ttctcaatga atcaagggcg tattgcaatg acagatggcg acaaaaaata
     2881 gcgtcagaag gagattgcaa aaaacatgca ctaccattga gatttactgt tggttgtata
     2941 gttttttctc tatggatttt tttagacagt ttcatacaat ggttacgatc ttcggcagtt
     3001 aactgttcct gctcaatata atccagtatg cttcgcaaat ggttagcctt aataggcgtg
     3061 gtttccagta ataaggaaat gaaatctttt ttatagtgta atgtaatgag tatattttct
     3121 gcatggatgg gtgaagtggc aaagttaatg gtaaaaaggg cagtggaata acaattttgt
     3181 gatatttttc gacagtttgc ggtaaattcc tgaataacac agtatgacag catcgaactt
     3241 aagtcagagg tatcaagtag ggtgatgagc tggcgcgcaa atttatcaat aggctctgat
     3301 gaaggtgttg gtatgtcgct atcgatgtgc aatctatgag gagacttata atgagttctg
     3361 ataatgatat cgttttgact cataatctat tcgccttata tatatctgga ggttttgagg
     3421 atgatgatta attattatcg ccagaacgaa atgtgcacga aaaattgtta taagaattgt
     3481 gtcactttta tcattgtgat atgaccactt cgtcattaat ctcatgagcg tttaaaatac
     3541 taccgccaaa tcacgcaacg aactttgctg gattcagttt atgctgctgt aaatccgctc
     3601 gcagtaaacc tttcagacgc accgtgatgt tatcagtggt tctttaagcg ttttgctggt
     3661 gtactcacta caaacgaatt gcgaatcgaa tcaatgtgaa acggaaaggt acaatctccc
     3721 tttttttagt gttaacatcg ctcaaccggg tggaaggtga gaggttaatg tctttcattt
     3781 gtgtacctga ttgcgaacca atttagcatt tgttggctag atggtttcgc aatgaactgt
     3841 taataaacaa atttttcttt gtatgtgatc tttcgtgtgg gtcaccactg caaataagga
     3901 tataaaatgc ctgttataac tcttcctgat ggcagccaac gccattacga ccacgctgta
     3961 agccccatgg atgttgcgct ggacattggt ccaggtctgg cgaaagcctg tatcgcaggg
     4021 cgcgttaatg gcgaactggt tgatgcttgc gatctgattg aaaacgacgc acaactgtcc
     4081 atcattacca ccaaagacga agacggtctg gagatcattc gtcactcctg tgcgcacctg
     4141 ttagggcacg cgattaaaca actttggccg cataccaaaa tggcaatcgg cccggttatt
     4201 gacaacggtt tttattacga cgttgatctt gaccgcacgt taacccagga agatgtcgaa
     4261 gcactcgaga agcggatgca tgagcttgct gagaaaaact acgacgtcat taagaagaaa
     4321 gtcagctggc acgaagcgcg tgaaactttc gccaaccgtg gggagagcta caaagtctcc
     4381 attcttgacg aaaacatcgc ccatgatgac aagccaggtc tgtacttcca tgaagaatat
     4441 gtcgatatgt gccgcggtcc gcacgtaccg aacatgcgtt tctgccatca tttcaaacta
     4501 atgaaaacgg caggggctta ctggcgtggc gacagcaaca acaaaatgtt gcaacgtatt
     4561 tacggtacgg cgtgggcaga caaaaaagcg cttaacgctt acctgcagcg cctggaagaa
     4621 gccgcgaaac gcgaccaccg taaaatcggt aaacagctcg acctgtacca tatgcaggaa
     4681 gaagcgccgg gtatggtatt ctggcacaac gacggctgga ccatcttccg tgaactggaa
     4741 gtgtttgttc gttctaaact gaaagagtac cagtatcagg aagttaaagg tccgttcatg
     4801 atggaccgtg tcctgtggga aaaaaccggt cactgggaca actacaaaga tgcaatgttc
     4861 accacgtctt ctgagaaccg tgaatactgc attaagccga tgaactgccc gggtcacgta
     4921 caaattttca accaggggct gaagtcttat cgcgatctgc cgctgcgtat ggccgagttt
     4981 ggtagctgcc accgtaatga gccgtcaggt tcgctgcatg gcctgatgcg cgtgcgtgga
     5041 tttacccagg atgacgcgca tatcttctgt actgaagaac aaattcgcga tgaagttaac
     5101 ggatgtatcc gtttagtcta tgatatgtac agcacttttg ggttcgagaa gatcgtcgtc
     5161 aaactctcca ctcgtcctga aaaacgtatt ggcagtgatg aaatgtggga tcgtgctgag
     5221 gcggacctgg cggttgcgct ggaagaaaac aacatcccgt ttgaatatca actgggtgaa
     5281 ggcgctttct acggtccgaa aattgaattt accctgtatg actgcctcga tcgtgcatgg
     5341 cagtgcggta cagtacagct ggacttctct ttgccttctc gtctgagcgc ttcttatgta
     5401 ggcgaagaca atgaacgtaa agtaccggta atgattcacc gcgcaattct ggggtcgatg
     5461 gaacgtttca tcggtatcct gaccgaagaa ttcgctggtt tcttcccgac ctggcttgcg
     5521 ccagttcagg ttgttatcat gaatattacc gattcacagt ctgaatacgt taacgaattg
     5581 acgcaaaaac tatcaaatgc gggcattcgt gttaaagcag acttgagaaa tgagaagatt
     5641 ggctttaaaa tccgcgagca cactttgcgt cgcgtcccat atatgctggt ctgtggtgat
     5701 aaagaggtgg aatcaggcaa agttgccgtt cgcacccgcc gtggtaaaga cctgggaagc
     5761 atggacgtaa atgaagtgat cgagaagctg caacaagaga ttcgcagccg cagtcttaaa
     5821 caattggagg aataaggtat taaaggcgga aaacgagttc aaacggcgcg ccctaaccgt
     5881 atcaatggcg aaattcgcgc ccaggaagtt cgcttaacag gtctggaagg cgagcagctt
     5941 ggtattgtga gtctgagaga agctctggag aaagcagaag aagccggagt agacttagtc
     6001 gagatcagcc ctaacgccga gccgccggtt tgtcgtataa tggattacgg caaattcctc
     6061 tatgaaaaga gcaagtcttc taaggaacag aagaaaaagc aaaaagttat ccaggttaag
     6121 gaaattaaat tccgtcctgg tacagatgaa ggtgactatc aggtaaaact ccgcagcctg
     6181 attcgctttc tcgaagaggg tgataaagcc aaaatcacgc tgcgtttccg cggtcgtgag
     6241 atggcgcacc agcaaatcgg tatggaagtg cttaatcgcg tgaaagacga tttgcaagaa
     6301 ctggcagtgg tcgaatcctt cccaacgaag atcgaaggcc gccagatgat catggtgctc
     6361 gctcctaaga agaaacagta aggccttcaa gtagcactgt ctgtggagcc ttcgggcttc
     6421 acaggctttg ttcgcctttg tttcgtttat taacaatgcg aagtggaagt tattaaaatg
     6481 ccaaaaatta agaccgtacg cggtgctgct aagcgcttca aaaaaaccgg taaaggtggt
     6541 tttaagcaca agcacgctaa cctgcgtcac attctgacca aaaaagcgac caaacgtaaa
     6601 cgtcacctgc gtccgaaagc catggtttcc aaaggcgatc tgggcctggt aatcgcgtgc
     6661 ctgccgtacg cataagccgt taacgttttt aactttttaa ttagaatata gatacaggag
     6721 agcacatatg gctcgcgtaa aacgtggtgt tattgcacgc gcacgtcaca agaaaatttt
     6781 gaaacaagct aaaggctact acggtgcgcg ttctcgcgta taccgcgttg ccttccaggc
     6841 tgttatcaaa gctggtcagt atgcttaccg tgaccgtcgt caacgtaagc gtcagttccg
     6901 tcaactgtgg attgcgcgta tcaacgcagc agcacgtcag aacggtattt cttacagcaa
     6961 attcatcaat ggcctgaaaa aagcctctgt tgaaatcgac cgtaagatcc tggctgatat
     7021 cgcagtattc gacaaagtag cgttcaccgc tctggttgaa aaagcgaaag cagctctggc
     7081 ataagccagt tgaaagaggg agctagtctc cctcttttcg tttcaacgcc atcaaaacat
     7141 tgacttttat cgccgtagcc ttttcaataa aggtcttttg aagagtacca aaaggtaacg
     7201 caagcaatga atgctgctat tttccgcttc tttttttact ttagcacctg aatccaggag
     7261 gctagcgcgt gagaagagaa acggaaaaca gcgcctgaaa gcctcccagt ggaggctttt
     7321 tttgtatgcg cgttttgaaa aataaaaagc ctgtctcatc aggtgtaatt gtcgcagcca
     7381 gtctggacac ggccagcgcg gagaaaccgg agcgtattta agtacgtgag aatttcgagc
     7441 actgcccggg accaaaatgg caagtaaaat agcctgatgg gataggctct aagtccaacg
     7501 aaccagtgtc accactgaca caatgaggaa aaccatgtca catctcgcag aactggttgc
     7561 cagtgcgaag gcggccatta gccaggcgtc agatgttgcc gcgttagata atgtgcgcgt
     7621 cgaatatttg ggtaaaaaag ggcacttaac ccttcagatg acgaccctgc gtgagctgcc
     7681 gccagaagag cgtccggcag ctggtgcggt tatcaacgaa gcgaaagagc aggttcagca
     7741 ggcgctgaat gcgcgtaaag cggaactgga aagcgcagcg ctgaatgcgc gtctggcggc
     7801 ggaaacgatt gatgtctccc tgccaggtcg tcgcattgaa aacggcggtc tgcatccggt
     7861 tacccgtacc atcgaccgta tcgaaagttt cttcggtgag cttggcttta ccgtggcaac
     7921 cgggccggaa atcgaagacg attatcataa cttcgatgct ctgaacattc ctggtcacca
     7981 cccggcgcgc gctgaccacg acactttctg gtttgacact acccgcctgc tgcgtaccca
     8041 gacctctggc gtacagatcc gcaccatgaa agcccagcag ccaccgattc gtatcatcgc
     8101 gcctggccgc gtttatcgta acgactacga ccagactcac acgccgatgt tccatcagat
     8161 ggaaggtctg attgttgata ccaacatcag ctttaccaac ctgaaaggca ctctgcacga
     8221 tttcctgcgt aacttctttg aggaagattt gcagattcgc ttccgtcctt cctacttccc
     8281 gtttaccgag ccttctgcag aagttgacgt catgggtaaa aacggtaaat ggctggaagt
     8341 gctgggctgc gggatggtgc atccgaacgt gctgcgtaac gttggcatcg acccggaagt
     8401 ttactctggt ttcgccttcg ggatggggat ggagcgtctg actatgttgc gttacggcgt
     8461 caccgacctg cgttcattct tcgaaaacga tctgcgtttc ctcaaacagt ttaaataagg
     8521 caggaataga ttatgaaatt cagtgaactg tggttacgcg aatgggtgaa cccggcgatt
     8581 gatagcgatg cgctggcgaa tcaaatcact atggcgggcc tggaagttga cggtgtagaa
     8641 ccggttgccg gtagcttcca cggcgtggtc gttggtgaag tggttgagtg tgcgcagcat
     8701 ccgaacgctg acaaactgcg tgtgacaaaa gtgaatgtcg gcggcgatcg cctgctggac
     8761 atcgtctgtg gtgcgccaaa ctgccgtcag ggcctgcgtg tggcggtagc taccattggt
     8821 gctgttctgc cgggtgattt caaaattaaa gcggcgaaac tgcgtggcga accgtctgaa
     8881 gggatgctgt gctccttttc tgagctggga atttctgacg atcacagtgg cattatcgaa
     8941 ctgcctgcgg atgcgccgat tggcaccgac atccgcgaat acctgaaact cgatgacaac
     9001 accat
//
LOCUS       DAMZET010000168         8939 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.168, whole genome shotgun sequence.
ACCESSION   DAMZET010000168 DAMZET010000000
VERSION     DAMZET010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.168"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(142..810)
                     /locus_tag="POB34_003931"
     CDS             complement(142..810)
                     /locus_tag="POB34_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDI5920791.1"
                     /translation="MAQAAARGQLDLHYQPLVDLRDHRIAGAEALMRWRHPRLGLLPP
                     GQFLPLAESFGLMPEIGAWVLGEACRQMHKWQGPAWQPFRLAINVSASQVGPTFDDEV
                     KRVLADMALPAELLEIELTESVAFGNPALFASFDALRAIGVRFAADDFGTGYSCLQHL
                     KCCPITTLKIDQSFVARLPDDARDQTIVRAVIQLAHGLGMDVIFRRRLHQLIGRNGCC
                     AASS"
     gene            complement(846..1082)
                     /gene="merE"
                     /locus_tag="POB34_003932"
     CDS             complement(846..1082)
                     /gene="merE"
                     /locus_tag="POB34_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum mercury transporter MerE"
                     /protein_id="HDI5920792.1"
                     /translation="MNAPDKLPPETRQPVSGYLWGALAVLTCPCHLPILAAVLAGTTA
                     GAFLGEHWGVAALALTGLFVLAVTRLLRAFRGGS"
     gene            complement(1079..1441)
                     /gene="merD"
                     /locus_tag="POB34_003933"
     CDS             complement(1079..1441)
                     /gene="merD"
                     /locus_tag="POB34_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance co-regulator MerD"
                     /protein_id="HDI5920793.1"
                     /translation="MSAYTVSQLAHNAGVSVHIVRDYLVRGLLRPVACTTGGYGVFDD
                     AALQRLCFVRAAFEAGIGLDALARLCRALDAADGAQAAAQLAVLRQLVERRRAALAHL
                     DAQLASMPAERAHEEALP"
     gene            complement(1459..3153)
                     /locus_tag="POB34_003934"
     CDS             complement(1459..3153)
                     /locus_tag="POB34_003934"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HDI5920794.1"
                     /translation="MSTLKITGMTCDSCAVHVKDALEKVPGVQSADVSYAKGSAKLAI
                     EVGTSPDALTAAVAGLGYRATLADAPSVSTPGGLLDKMRDLLGRNDKTGSSGALHIAV
                     IGSGGAAMAAALKAVEQGARVTLIERGTIGGTCVNVGCVPSKIMIRAAHIAHLRRESP
                     FDGGIAATTPTIQRTALLAQQQARVDELRHAKYEGILEGNPAITVLHGSARFKDNRNL
                     IVQLNDGGERVVAFDRCLIATGASPAVPPIPGLKDTPYWTSTEALVSETIPKRLAVIG
                     SSVVALELAQAFARLGAKVTILARSTLFFREDPAIGEAVTAAFRMEGIEVREHTQASQ
                     VAYINGEGDGEFVLTTAHGELRADKLLVATGRAPNTRKLALDATGVTLTPQGAIVIDP
                     GMRTSVEHIYAAGDCTDQPQFVYVAAAAGTRAAINMTGGDAALNLTAMPAVVFTDPQV
                     ATVGYSEAEAHHDGIKTDSRTLTLDNVPRALANFDTRGFIKLVVEEGSGRLIGVQAVA
                     PEAGELIQTAALAIRNRMTVQELADQLFPYLTMVEGLKLAAQTFNKDVKQLSCCAG"
     gene            complement(3205..3627)
                     /gene="merC"
                     /locus_tag="POB34_003935"
     CDS             complement(3205..3627)
                     /gene="merC"
                     /locus_tag="POB34_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial transporter MerC"
                     /protein_id="HDI5920795.1"
                     /translation="MGLMTRIADKTGALGSVVSAMGCAACFPALASFGAAIGLGFLSQ
                     YEGLFISRLLPLFAALAFLANALGWFSHRQWLRSLLGMIGPAIVFAATVWLLGNWWTA
                     NLMYVGLALMIGVSIWDFVSPAHRRCGPDGCELPAKRL"
     gene            complement(3663..3938)
                     /gene="merP"
                     /locus_tag="POB34_003936"
     CDS             complement(3663..3938)
                     /gene="merP"
                     /locus_tag="POB34_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance system periplasmic binding
                     protein MerP"
                     /protein_id="HDI5920796.1"
                     /translation="MKKLFASLALAAAVAPVWAATQTVTLAVPGMTCAACPITVKKAL
                     SKVEGVSKVDVGFEKREAVVTFDDTKASVQKLTKATADAGYPSSVKQ"
     gene            complement(3952..4302)
                     /locus_tag="POB34_003937"
     CDS             complement(3952..4302)
                     /locus_tag="POB34_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric transport protein MerT"
                     /protein_id="HDI5920797.1"
                     /translation="MSEPQNGRGALFAGGLAAILASTCCLGPLVLVALGFSGAWIGNL
                     TVLEPYRPLFIGAALVALFFAWKRIYRPVQACKPGEVCAIPQVRATYKLIFWIVAVLV
                     LVALGFPYVVPFFY"
     gene            4374..4808
                     /gene="merR"
                     /locus_tag="POB34_003938"
     CDS             4374..4808
                     /gene="merR"
                     /locus_tag="POB34_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HDI5920798.1"
                     /translation="MENNLENLTIGVFAKAAGVNVETIRFYQRKGLLREPDKPYGSIR
                     RYGEADVVRVKFVKSAQRLGFSLDEIAELLRLDDGTHCEEASSLAEHKLKDVREKMAD
                     LARMETVLSELVCACHARKGNVSCPLIASLQGEAGLARSAMP"
     gene            5811..6053
                     /locus_tag="POB34_003939"
     CDS             5811..6053
                     /locus_tag="POB34_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HDI5920799.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(6085..6735)
                     /gene="tetR(A)"
                     /locus_tag="POB34_003940"
     CDS             complement(6085..6735)
                     /gene="tetR(A)"
                     /locus_tag="POB34_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HDI5920800.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            6841..8040
                     /gene="tet(A)"
                     /locus_tag="POB34_003941"
     CDS             6841..8040
                     /gene="tet(A)"
                     /locus_tag="POB34_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HDI5920801.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(8072..8932)
                     /locus_tag="POB34_003942"
     CDS             complement(8072..8932)
                     /locus_tag="POB34_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDI5920802.1"
                     /translation="MFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALPAGLLLVMI
                     VRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVVFISAAL
                     LGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLTRKWQP
                     PVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYFLWFR
                     GISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSNRTP
                     RARIACRKSP"
BASE COUNT         1664 a         2814 c         2830 g         1631 t
ORIGIN      
        1 gctcccaagc ctcatcaggc agagtggcca cgccttgttc tggaatccgt ggggtgtccg
       61 tcgccatgct cacctcgctt tggtgcacac gagtattgag catagtcgag attggtgcag
      121 atcacttctg atattgaact gtcaggagct ggctgcacaa cagccattac gcccaatcaa
      181 ctggtgcagt cgtcttctga aaatgacatc catgcccagc ccgtgcgcga gctggatcac
      241 cgcccgcacg atagtttggt cacgggcatc atccgggagc ctggcgacaa aggattggtc
      301 gattttcaat gtggtgatgg ggcagcattt cagatgttgc aggcaggaat agccggtgcc
      361 gaagtcgtcg gcggcgaagc gcacgccgat ggcgcgcaag gcgtcgaaac tggcgaacag
      421 ggctggattg ccgaatgcga ccgattcggt cagttcgatc tccagaagct cggcgggcag
      481 ggccatatcg gccagcaccc gctttacctc gtcgtcgaac gttggcccaa cctggctggc
      541 ggacacattg atggcaagac ggaacggttg ccatgccggt ccttgccact tgtgcatctg
      601 gcgacaggcc tcgcccagca cccacgcgcc tatttccggc atcaggccga acgactcggc
      661 cagcggcagg aactggccgg gcggcaacag gccaagcctc ggatgccgcc agcgcatcaa
      721 cgcttccgcg ccagcgatcc ggtgatcgcg cagatcgacc agcggctggt aatgcaggtc
      781 aagctgtccg cgcgccgccg cctgcgccaa ctcggccgcc gtccatccgg cgggctgcga
      841 actcgtcatg atccgccccg gaaggcgcgc agcagccgcg ttacggccag aacgaacaag
      901 ccggtcagcg cgagcgcggc aacaccccaa tgctcgccaa ggaaggcacc ggcggtcgtc
      961 ccggccagca cggcggcgag aatcggcaga tggcaggggc aggtcaacac ggccagcgca
     1021 ccccacaggt agccggaaac gggttggcgc gtctcgggcg gcagtttgtc aggggcgttc
     1081 acggcaatgc ctcctcgtgc gcccgctcgg ctggcatgga ggccagttgc gcgtccagat
     1141 gggccaacgc cgcgcgccgc cgctcgacca actggcgcag cacggcaagc tgcgctgcgg
     1201 cttgtgcgcc gtccgctgcg tcgagcgcac ggcacagccg cgccagggca tccaggccga
     1261 tacccgcctc gaaggccgcg cgcacgaagc acagccgttg caaggccgca tcgtcgaaca
     1321 cgccgtagcc gcccgtggtg caggccaccg gccgtaacaa gccgcgcacc aggtagtcgc
     1381 gcacgatatg tacgctcacc ccagcgttat gggccagttg cgataccgtg taggcgctca
     1441 tcgcacacct ccttgtcctc acccggcgca gcaggaaagc tgcttcacat ccttgttgaa
     1501 ggtctgcgcc gcgagcttca acccttcgac catcgtcagg taggggaaca actggtcggc
     1561 cagttcctgc accgtcatcc ggttgcgaat cgccagtgcg gccgtctgga tcagttcgcc
     1621 cgcttccggg gccactgcct gcacgccgat cagtcgtccg ctgccttctt caaccaccag
     1681 tttgatgaag ccgcgcgtgt cgaagttggc gagcgcgcgc ggcacgttgt ccagcgttag
     1741 cgtgcgacta tcagttttga tgccgtcatg gtgcgcttcc gcctcgctgt agcctacggt
     1801 cgccacttgc gggtcggtga acaccacggc cggcatcgcg gtcaggttca gggccgcgtc
     1861 accgccggtc atgttgatcg cggcgcgagt gccggccgct gccgccacat agacgaactg
     1921 cggctggtcg gtgcagtcgc ctgcggcgta gatgtgttcc acgcttgtac gcatgccggg
     1981 gtcgatgacg atagcgcctt gcggggtgag cgtgacgccc gtcgcatcca gtgccagctt
     2041 gcgtgtgttg ggcgcgcggc cggtggcgac cagcagcttg tcggcgcgca gttcgccgtg
     2101 cgccgtggtg agcacgaatt cgccgtcccc ttcaccattg atatacgcga cctggctggc
     2161 ctgggtgtgt tccctcacct cgatgccctc catgcggaat gcggccgtga cggcttcgcc
     2221 tatagctggg tcttcgcgga agaacagcgt gctgcgagcc aggatcgtca ccttcgctcc
     2281 gagtcgggcg aacgcctgcg ccagctccag cgccaccact gatgagccaa tcacggccag
     2341 gcgcttagga atcgtctcgc tgaccagcgc ttcagtggaa gtccagtacg gagtgtcttt
     2401 caggccggga atcggcggca cggccgggct cgcgccggtg gcgatcaggc agcggtcgaa
     2461 tgccaccacg cgctcgccgc cgtcgttgag ttgcacgatc aggttgcgat tgtccttaaa
     2521 gcgggcggag ccgtgcagca cagtgatcgc cggattgccc tccaagatgc cttcgtactt
     2581 ggcgtggcgc agttcatcga cgcgggcctg ctgctgggcc agcagcgccg tgcgctggat
     2641 ggtcggcgtg gtagcggcga tgccgccatc gaacgggctt tcccggcgca gatgggcgat
     2701 atgggcggcg cggatcatga tcttggacgg cacacaaccg acattgacgc aggtgccgcc
     2761 gatggtgccg cgctcgatca gcgtgacacg tgcgccttgc tcgacggcct tcagcgccgc
     2821 tgccatcgcg gccccgccgc tgccgatgac ggcgatatgc aatgcgccgc tgctacccgt
     2881 cttgtcgttt ctgcccagca gatcgcgcat cttgtcgagc aatccgcccg gcgtcgaaac
     2941 tgagggggca tcggccagcg tggcccgata accgagtcca gctacagcgg ccgtcagcgc
     3001 gtcgggtgac gtgccgacct caatggcgag cttggcgctg cccttggcgt aggagacatc
     3061 cgctgattgc acgccgggca ctttctccag ggcgtccttg acatgcactg cgcacgagtc
     3121 gcaagtcatg ccggtgattt tgagagtgct cataccatcg ttccttattc gtgtgggccg
     3181 ccgtgtcgca cggtcagccg tctttcacaa gcgcttggcg gggagttcgc agccgtccgg
     3241 tccgcaacgg cgatgcgccg gcgacacgaa gtcccagatc gacaccccaa tcatcaaggc
     3301 caggccgacg tacatcaggt tcgccgtcca ccagttgccg agcagccaga ccgtggccgc
     3361 aaacacgatg gccgggccga tcatgccgag cagactgcgc agccattgcc gatgactgaa
     3421 ccaacccagc gcgttcgcca ggaaggccag cgcggcaaac agcggcagca ggcggctgat
     3481 gaacagtccc tcgtactggc tcaagaagcc cagcccgatg gccgcgccga agctggcgag
     3541 ggctggaaag caggcggcgc agcccatcgc ggaaacgacg ctgccgagcg cgccggtttt
     3601 atcggcaatg cgtgtcatca gtcccatgaa gcggctctcg ctgttgtcgt tggcttgctg
     3661 gctcactgct tgacgctgga cggatagccg gcgtctgcgg tggccttggt cagcttctgt
     3721 acgctggcct tggtgtcgtc aaaagtgacg acggcctcgc gcttctcgaa gcccacatcg
     3781 accttgctca cgccttcgac cttggagagc gctttcttga ctgtgatcgg gcaggcggcg
     3841 caagtcatgc cgggaaccgc tagcgtgacg gtctgggtag cggcccacac cggggcaaca
     3901 gcggcggcga gggcaaggga ggcaaacagt ttcttcatga tgaactcctg gttaatagaa
     3961 aaatggaacg acatagggaa atccaagcgc gaccaggacc agcacggcca cgatccagaa
     4021 aatcagcttg taggtggcgc gcacctgcgg aatcgcgcag acctcacctg gcttgcatgc
     4081 ctgcacgggc cggtaaatcc gcttccaggc gaagaacagc gccactagcg ccgcgccgat
     4141 gaacaacggt cgatagggtt ccagcaccgt caggttgccg atccaagcac cggagaagcc
     4201 cagggcgacc agtactagcg gccccaggca gcaggtcgat gcaagaatgg cggccagccc
     4261 gccggcgaag agcgcaccgc gcccgttttg tggttcagac atacgttggc ccttttgaat
     4321 ttggattgga tagcgtaacc ttacttccgt actcatgtac ggagtcaagc gatatggaaa
     4381 ataatttgga aaacctgacc attggcgttt ttgccaaggc ggccggggtc aacgtggaga
     4441 caatccgctt ctatcagcgc aagggcctgt tgcgggaacc ggacaagcct tacggcagca
     4501 tccgccgcta tggggaggcg gacgtggttc gggtgaaatt cgtgaaatcg gcacagcggc
     4561 tggggttcag tctggacgag attgccgagc tgttgcggct cgacgatggc acccactgcg
     4621 aggaggccag cagcctggcc gaacacaagc tcaaggacgt gcgcgagaag atggccgact
     4681 tggcgcgcat ggaaaccgtg ctgtctgaac tcgtgtgcgc ctgccatgca cgaaagggga
     4741 atgtttcctg cccgttgatc gcgtcactac agggcgaagc aggcctggca aggtcagcta
     4801 tgccttagcg tgctttattt tccgttttct gaggtgcccc ctaattgagc atttccaggc
     4861 gcttgcgcct ggtcaacgaa agagtcagcg ccgtaggcgc tgccattttt ggggtgaggc
     4921 cgttcgcggc cgaggggcgc agcccctggg gggatgggag gcccgcgtta gcgggccggg
     4981 agggttcgag aagggggggc accccccttc ggcgtgcgcg gtcacgcgca cagggcgcag
     5041 ccctggttaa aaacaaggtt tataaatatt ggtttaaaag caggttaaaa gacaggttag
     5101 cggtggccga aaaacgggcg gaaacccttg caaatgctgg attttctgcc tgtggacagc
     5161 ccctcaaatg tcaataggtg cgcccctcat ctgtcagcac tctgcccctc aagtgtcaag
     5221 gatcgcgccc ctcatctgtc agtagtcgcg cccctcaagt gtcaataccg cagggcactt
     5281 atccccaggc ttgtccacat catctgtggg aaactcgcgt aaaatcaggc gttttcgccg
     5341 atttgcgagg ctggccagct ccacgtcgcc ggccgaaatc gagcctgccc ctcatctgtc
     5401 aacgccgcgc cgggtgagtc ggcccctcaa gtgtcaacgt ccgcccctca tctgtcagtg
     5461 agggccaagt tttccgcgag gtatccacaa cgccggcggc cgcggtgtct cgcacacggc
     5521 ttcgacggcg tttctggcgc gtttgcaggg ccatagacgg ccgccagccc agcggcgagg
     5581 gcaaccagcc cggtgagcgt cggaaaggcg ctggaagccc cgtagcgacg cggagagggg
     5641 cgagacaagc caagggcgca ggctcgatgc gcagcacgac atagccggtt ctcgcaagga
     5701 cgagaatttc cctgcggtgc ccctcaagtg tcaatgaaag tttccaacgc gagccattcg
     5761 cgagagcctt gagtccacgc tagatctatc tcatctgcgc aaggcagaac gtgaagacgg
     5821 ccgccctgga cctcgcccgc gagcgccagg cgcacgaggc cggcgcgcgg acccgcgcca
     5881 cggcccacga gcggacgccg cagcaggagc gccagaaggc cgccagagag gccgagcgcg
     5941 gccgtgaggc ttggacgcta gggcagggca tgaaaaagcc cgtagcgggc tgctacgggc
     6001 gtctgacgcg gtggaaaggg ggaggggatg ttgtctacat ggctctgctg tagtgagtgg
     6061 gttgcgctcc ggcagcggtc ctgatcaatc gtcacccttt ctcggtcctt caacgttcct
     6121 gacaacgagc ctccttttcg ccaatccatc gacaatcacc gcgagtccct gctcgaacgc
     6181 tgcgtccgga ccggcttcgt cgaaggcgtc tatcgcggcc cgcaacagcg gcgagagcgg
     6241 agcctgttca acggtgccgc cgcgctcgcc ggcatcgctg tcgccggcct gctcctcaag
     6301 cacggcccca acagtgaagt agctgattgt catcagcgca ttgacggcgt ccccggccga
     6361 aaaacccgcc tcgcagagga agcgaagctg cgcgtcggcc gtttccatct gcggtgcgcc
     6421 cggtcgcgtg ccggcatgga tgcgcgcgcc atcgcggtag gcgagcagcg cctgcctgaa
     6481 gctgcgggca ttcccgatca gaaatgagcg ccagtcgtcg tcggctctcg gcaccgaatg
     6541 cgtatgattc tccgccagca tggcttcggc cagtgcgtcg agcagcgccc gcttgttcct
     6601 gaagtgccag taaagcgccg gctgctgaac ccccaaccgt tccgccagtt tgcgtgtcgt
     6661 cagaccgtct acgccgacct cgttcaacag gtccagggcg gcacggatca ctgtattcgg
     6721 ctgcaacttt gtcatgcttg acactttatc actgataaac ataatatgtc caccaactta
     6781 tcagtgataa agaatccgcg cgttcaatcg gaccagcgga ggctggtccg gaggccagac
     6841 gtgaaaccca acagacccct gatcgtaatt ctgagcactg tcgcgctcga cgctgtcggc
     6901 atcggcctga ttatgccggt gctgccgggc ctcctgcgcg atctggttca ctcgaacgac
     6961 gtcaccgccc actatggcat tctgctggcg ctgtatgcgt tgatgcaatt tgcctgcgca
     7021 cctgtgctgg gcgcgctgtc ggatcgtttc gggcggcggc cggtcttgct cgtctcgctg
     7081 gccggcgctg ctgtcgacta cgccatcatg gcgacggcgc ctttcctttg ggttctctat
     7141 atcgggcgga tcgtggccgg catcaccggg gcgactgggg cggtagccgg cgcttatatt
     7201 gccgatatca ctgatggcga tgagcgcgcg cggcacttcg gcttcatgag cgcctgtttc
     7261 gggttcggga tggtcgcggg acctgtgctc ggtgggctga tgggcggttt ctccccccac
     7321 gctccgttct tcgccgcggc agccttgaac ggcctcaatt tcctgacggg ctgtttcctt
     7381 ttgccggagt cgcacaaagg cgaacgccgg ccgttacgcc gggaggctct caacccgctc
     7441 gcttcgttcc ggtgggcccg gggcatgacc gtcgtcgccg ccctgatggc ggtcttcttc
     7501 atcatgcaac ttgtcggaca ggtgccggcc gcgctttggg tcattttcgg cgaggatcgc
     7561 tttcactggg acgcgaccac gatcggcatt tcgcttgccg catttggcat tctgcattca
     7621 ctcgcccagg caatgatcac cggccctgta gccgcccggc tcggcgaaag gcgggcactc
     7681 atgctcggaa tgattgccga cggcacaggc tacatcctgc ttgccttcgc gacacgggga
     7741 tggatggcgt tcccgatcat ggtcctgctt gcttcgggtg gcatcggaat gccggcgctg
     7801 caagcaatgt tgtccaggca ggtggatgag gaacgtcagg ggcagctgca aggctcactg
     7861 gcggcgctca ccagcctgac ctcgatcgtc ggacccctcc tcttcacggc gatctatgcg
     7921 gcttctataa caacgtggaa cgggtgggca tggattgcag gcgctgccct ctacttgctc
     7981 tgcctgccgg cgctgcgtcg cgggctttgg agcggcgcag ggcaacgagc cgatcgctga
     8041 tcgtggaaac gataggccta tgccatgcgg gtcaaggcga cttccggcaa gctatacgcg
     8101 ccctaggagt gcggttggaa cgttggccca gccagatact cccgatcacg agcaggacgc
     8161 cgatgatttg aagcgcactc agcgtctgat ccaagaacaa ccatcctagc aacacggcgg
     8221 tccccgggct gagaaagccc agtaaggaaa caactgtagg ttcgagtcgc gagatccccc
     8281 ggaaccaaag gaagtaggtt aaacccgctc cgatcaggcc gagccacgcc aggccgagaa
     8341 cattggttcc tgtaggcatc gggattggcg gatcaaacac taaagctact ggaacgagca
     8401 gaagtcctcc ggccgccagt tgccaggcgg taaaggtgag cagaggcacg ggaggttgcc
     8461 acttgcgggt cagcacggtt ccgaacgcca tggaaaccgc ccccgccagg cccgctgcga
     8521 cgccgacagg atctagcgct gcgtttggtg tcaacaccaa cagcgccacg cccgcagttc
     8581 cgcaaatagc ccccaggacc gccatcaatc gtatcgggct acctagcaga gcggcagaga
     8641 tgaacacgac catcagcggc tgcacagcgc ctaccgtcgc cgcgaccccg cccggcaggc
     8701 ggtagaccga aataaacaac aagctccaga atagcgaaat attaagtgcg ccgaggatga
     8761 agatgcgcat ccaccagatt cccgttggaa tctgtcggac gatcatcacg agcaataaac
     8821 ccgccggcaa cgcccgcagc atcgcgaccg tcatcggtga gaagttcggc aggtattggg
     8881 tggtgacaat gtaggtgctg ccccaaatgg caggtgctat cgctgtgaac aataaatcg
//
LOCUS       DAMZET010000169         8832 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.169, whole genome shotgun sequence.
ACCESSION   DAMZET010000169 DAMZET010000000
VERSION     DAMZET010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.169"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1438)
                     /gene="rcsD"
                     /locus_tag="POB34_003943"
     CDS             complement(<1..1438)
                     /gene="rcsD"
                     /locus_tag="POB34_003943"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HDI5920803.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEQDATATGNNDNEKEGTDSVSIHFNS
                     TKIEIASALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQS"
     gene            2177..3280
                     /gene="ompC"
                     /locus_tag="POB34_003944"
     CDS             2177..3280
                     /gene="ompC"
                     /locus_tag="POB34_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDI5920804.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            3392..4447
                     /gene="apbE"
                     /locus_tag="POB34_003945"
     CDS             3392..4447
                     /gene="apbE"
                     /locus_tag="POB34_003945"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HDI5920805.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            4521..5585
                     /gene="ada"
                     /locus_tag="POB34_003946"
     CDS             4521..5585
                     /gene="ada"
                     /locus_tag="POB34_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HDI5920806.1"
                     /translation="MKKATCLTGDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPEKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALHTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            5585..6235
                     /gene="alkB"
                     /locus_tag="POB34_003947"
     CDS             5585..6235
                     /gene="alkB"
                     /locus_tag="POB34_003947"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HDI5920807.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTSHRQDYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            6311..7954
                     /gene="yojI"
                     /locus_tag="POB34_003948"
     CDS             6311..7954
                     /gene="yojI"
                     /locus_tag="POB34_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HDI5920808.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            8172..>8832
                     /locus_tag="POB34_003949"
     CDS             8172..>8832
                     /locus_tag="POB34_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HDI5920809.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQ"
BASE COUNT         2172 a         2127 c         2348 g         2185 t
ORIGIN      
        1 gggactgtgc aggttctttc agcgcatcgc caatgttttt cataaaggtc atccggccct
       61 gctggttttt ctcatacaga cgctgcgcct gcttgagttt cttgtttacc agcacttcgc
      121 gatcctgatc gcgaatgatg aaaatttgtg tgcgcggcgc gacctggctg cggaacatgc
      181 ggatctcata cagctcgtta ttgatcgtcg cctgaataat cccctgatgc tgttccgcca
      241 tggtggtgat gttttgcaga ttcaaatgcg gcagcaaatg atcggcaatt ttgttactta
      301 tgacagtgcg gttcgattcc tgatcgtgaa ccagcaggcc gagcggcagc agtgagacta
      361 tctcttcatt gattgcccgt aaaatgcgca attcgttatt gaccgccgtg ttgggtagac
      421 tttctgtact acgactggag aaatggcgga atgtggtata gccaaataac gccagcgcca
      481 gcaaaccgat gttcagcagc agtggcagca gaatgttttg caacgtatcc agcaataagg
      541 tgccataagg aacctgccag accaggcgca tatcggtaga gttgagtgcc gaggcgattt
      601 caatcttcgt actgttaaag tggatactga cgctatccgt cccttctttc tcattatcat
      661 tgtttcccgt tgccgtcgcg tcttgctcaa ggcggaaact gtccagcggc atacccggtg
      721 ggatcaaatc attaatcggc agatcaaaag ccacgaccgt tgccagatgt cccggctggt
      781 tgaacgtggt acgcaaggta aagtaatgac cgttctgcca ggccaggcgg cgcatgttag
      841 aaaaactttc gcgttcatcg agggcgttgg cctgttgcaa catttctgca cgacgtgaat
      901 caacaatgtc actgacggtc gattctttaa atccggaggt gagatctttg aggggcaggg
      961 ttgagatcag caccagactg ttatcctgac cattcaggta atacattgac cacggtacat
     1021 tttctgcgcc ccacaatgtg tccagatagg tggacatccg ttgagtcatc tcaagcgttg
     1081 agttgtcgtg agagccaaag attaacgctt ccgttttgcg gcgcggtttt tccagatagt
     1141 agacatcctg tttcaggcgc gtctcttgta aaccttcgcc ggaggagggg gaggtcgtcg
     1201 cggcaatatt gtcgtagatc tgccaggtca cgtaacgcca ggtatcgacg cgcttttgga
     1261 tagcgtgggt aatgtcgaca atctggtaac ttttatcttt cagccaggcg ttaacggcgc
     1321 tttgtaccat tacacccatc gtcaccagta acacaatgat caacagtaaa aagaagcggg
     1381 taatgctccc cggtaggagt gaaaagcggg tcgtggccgt tgtctctttc tgacgcattc
     1441 gtgtttatga cctgttaaaa cttcgcgaag tgatagggca gtataaaggg tacagtgtga
     1501 aattccagac tcttacctga ttgatgattc tctattctcc cgtgatattt atcatcctgc
     1561 attgggggaa atagatgtac aagcgccatt ttgctgtaca aataacctac aaaaagccca
     1621 acaaaaataa ttgcaaataa acaaagattg ctggaaatta tgctgatgtt aattatttgt
     1681 gaaatagtta acaagcgtta tagtttttct gtggtagcac agaataatga aaagtgtgta
     1741 aagaagggta aaaaaaaccg gatgcgaggc atccggttga aataggggta aacagacatt
     1801 cagaaatgaa tgacggtaat aaataaagtt aatgatgata gcgggagtta ttctagttgc
     1861 gagtgaaggt tttgttttga cattcagtgc tgtcaaatac ttaagaataa gttattgatt
     1921 ttaaccttga attattattg cttgatgtta ggtgcttatt tcgccattcc gcaataatct
     1981 taaaaagttc ccttgcattt acattttgaa acatctatag cgataaatga aacatcttaa
     2041 aagttttagt atcatattcg tgttggatta ttctgcattt ttggggagaa tggacttgcc
     2101 gactgattaa tgagggttaa tcagtatgca gtggcataaa aaagcaaata aaggcatata
     2161 acagagggtt aataacatga aagttaaagt actgtccctc ctggtcccag ctctgctggt
     2221 agcaggcgca gcaaacgctg ctgaagttta caacaaagac ggcaacaaat tagatctgta
     2281 cggtaaagta gacggcctgc actatttctc tgacaacaaa gatgtagatg gcgaccagac
     2341 ctacatgcgt cttggcttca aaggtgaaac tcaggttact gaccagctga ccggttacgg
     2401 ccagtgggaa tatcagattc agggcaacag cgctgaaaac gaaaacaact cctggacccg
     2461 tgtggcattc gcaggtctga aattccagga tgtaggttct ttcgactacg gtcgtaacta
     2521 cggcgttgtt tacgacgtaa cttcctggac cgacgtactg ccagaattcg gtggcgacac
     2581 ctacggttct gacaacttca tgcagcagcg tggtaacggc ttcgcgacct accgtaacac
     2641 tgacttcttc ggtctggttg acggcctgaa ctttgctgtt cagtaccagg gtaaaaacgg
     2701 tagcgtaagc ggcgaaggca tgaccaacaa cggtcgtggt gctctgcgtc agaacggcga
     2761 cggcgttggc ggatctatca cttatgatta cgaaggcttc ggtatcggtg gtgcgatctc
     2821 cagctccaaa cgtactgatg atcagaacag cccgctgtac atcggtaacg gcgaccgtgc
     2881 tgaaacctac accggtggtc tgaaatacga cgctaacaac atctacctgg ctgctcagta
     2941 cacccagacc tataacgcaa ctcgcgtagg ttccctgggt tgggcgaaca aagcacagaa
     3001 ctttgaagct gttgctcagt accagttcga cttcggtctg cgtccgtctg tagcatacct
     3061 gcagtctaaa ggtaaaaacc tgggtaccat cgctggtcgt aactacgacg acgaagatat
     3121 cctgaaatat gttgatgttg gtgctaccta ctacttcaac aaaaacatgt ccacctacgt
     3181 tgactacaaa atcaacctgc tggacgacaa ccagttcact cgtgacgctg gcatcaacac
     3241 tgataacatc gtagctctgg gtctggttta ccagttctaa tctcgattga tatcgaacaa
     3301 agggcctgcg ggcccttttt tcattgtttt cagcgtacaa acacagtttt ttggtgtact
     3361 cttgcgaccg ttcgcatgag gataatcacg tatggaaata agctttaccc gcgtggcact
     3421 gctggctgcc gcgctctttt ttgttggttg cgatcaaaaa ccacaacccg ccaaaaccca
     3481 cgctactgaa gttaccgttc ttgaaggcaa aaccatgggt accttctggc gtgccagcat
     3541 cccgggcatt gatgccaaac gcagtgccga acttaaagaa aagattcaga cccagctgga
     3601 cgccgacgat cagctgcttt cgacctataa aaaagattcc gcgctgatgc gctttaacga
     3661 ttcgcaaagt ttatcgccgt ggccggtaag tgaagcgatg gccgatatcg tcaccacctc
     3721 gctgcgcatt ggcgcgaaga ccgatggcgc gatggatata accgtcgggc cgctggtgaa
     3781 tctgtggggc tttggcccgg aacaacagcc ggttcaaatt ccgagccagg aacagatcga
     3841 tgcgatgaaa gccaaaaccg gcttacagca cctgacggtc attaatcagt cgcatcagca
     3901 atatctgcaa aaagacctgc cggatttaaa tgtcgatctc tctaccgtcg gcgaaggtta
     3961 tgcggcggat cacttggcac gcttgatgga gcaggaaggg atttcccgct atctggtgtc
     4021 ggtgggcggc gcgctgaaca gccgtggtat gaacggtgaa ggccagccgt ggcgggtagc
     4081 gattcaaaaa ccaaccgata aagaaaacgc ggttcaggct gtggtggata tcaacggtca
     4141 tgggattagc acctctggca gctaccgtaa ctattacgaa ctggacggca aacgtctttc
     4201 ccatgttatc gatccgcaaa ccgggcgtcc catcgaacac aatctggtat ccgtgacggt
     4261 gattgctccg acggcgctgg aagccgatgc ctgggatact ggcttgatgg tactcgggcc
     4321 ggagaaagcc aaagaggttg ttcgccggga agggctggcg gtttatatga tcaccaaaga
     4381 aggcgatagc tttaaaacct ggatgtcacc acagtttaaa agcttccttg tcagcgaaaa
     4441 aaattaaagc gcaagattgt tggtttttgc gtgatggtga ccgggcagac taaaggctat
     4501 ccttaaccag ggagctgatt atgaaaaaag ccacatgctt aactggcgat caacgctggc
     4561 aatctgtctt agcccgcgac ccgaatgccg acggcgaatt cgttttcgcc gtgcgtacca
     4621 caggcatctt ttgccgtccg tcttgccgcg ccagacatgc tttgcgggaa aacgtctcct
     4681 tctacgcaaa tgccagcgag gcactcgccg ctggctttcg cccctgcaaa cgttgtcagc
     4741 cagaaaaagc caatgcccag caacatcggt tggataaaat cacccacgcg tgtcgactgc
     4801 tggaacagga aacgcctgta acgttggaag ccttagccga ccaggtggcg atgagtccat
     4861 ttcatctaca tcggttgttt aaagcgacta ccggaatgac gcctaaagcc tggcaacagg
     4921 cctggcgcgc tcgccgtttg cgcgaatcgc tggcgaaagg ggagagcgtg acgacgtcta
     4981 ttcttaacgc cggattcccc gacagcagca gttactatcg caaagctgac gaaacgctgg
     5041 gcatgacggc taaacaattc cgtcacggtg gcgaaaatct ggcggtgcgt tacgcgctgg
     5101 ctgattgtga gctgggtcgt tgcctggtgg cagaaagcga gcgggggatt tgcgcgatat
     5161 tgctgggcga tgatgacgcg acactaatca gcgagttgca gcagatgttt cccgctgccg
     5221 acaacgcgcc tgccgatctg atgtttcagc aacatgtgcg tgaagtgatc gccagcctca
     5281 atcaacgcga tacgccgctg acgttaccgc tggacattcg cggcactgct tttcagcaac
     5341 aagtctggca ggcactgcac acgatacctt gcggtgaaac cgtcagttat cagcaactgg
     5401 ctaacgccat cggcaaaccg aaagcggtac gggccgttgc cagcgcctgt gccgccaaca
     5461 agctggctat cataataccc tgtcatcggg tggtccgtgg tgatggcaca ctttccggtt
     5521 accgctgggg cgtgtcgcgt aaagcgcaac tgctgcgccg cgaagctgaa aatgaggaga
     5581 ggtaatgttg gatctgtttg ccgatgctga accgtggcaa gagccactgg cggctggagc
     5641 ggtaattttg caccgttttg cttttaacgc tgcggagcaa ctaatccgcg atattaatga
     5701 cgttgccagc cagtcgccgt ttcgccagat ggtcaccccc gggggatata ccatgtcggt
     5761 ggcgatgacc aactgtgggc atctgggctg gacgagccat cggcaagatt atctctattc
     5821 gcccattgat ccgcaaacaa ataaaccgtg gcccgccatg ccacagagtt ttcataattt
     5881 atgtcaacgt gcggctacgg cggcgggcta tccagatttc cagccagatg cttgtcttat
     5941 caaccgctac gctcctggcg cgaaactgtc gctgcatcag gataaagacg aaccggatct
     6001 gcgcgcgcca attgtttctg tttctctggg cttacccgcg atttttcaat ttggtggcct
     6061 gaaacgtaat gatccgctca aacgtttgtt gttggaacat ggcgatgtgg tggtatgggg
     6121 cggtgaatcg cggctgtttt atcacggtat tcaaccgttg aaagcggggt ttcatccact
     6181 caccaccgac tgccgctaca acctgacatt ccgtcaggca ggtaaaaaag aataaaaata
     6241 agaattatta ttgctgtgcg cgcgaagatg tttaaactgc gggctgtaat tcattgtccg
     6301 ggttttctgc atggaacttc ttgtacttgt ctggcggcag tatcgctggc catttatcag
     6361 tgtgatggcg ctaagcctcg ccagtgcggc attaggcatt ggcttaattg cttttatcaa
     6421 tcagcgcctt atcgaaacgg cggataccag tctgctggtg ttgccggagt ttcttggatt
     6481 attactgctg ttgatggcag tcactctcgg atcgcaactg gcgctcacca ctttggggca
     6541 tcacttcgtt taccgactgc gtagtgaatt tatcaagcgg attctggata cccacgtcga
     6601 gcgcattgaa caactcggta gcgcctcgtt gctggcggga ttaaccagcg atgtgcgcaa
     6661 tatcaccatt gcttttgtgc gtcttccgga actggtgcag gggatcattc tcactatcgg
     6721 ttccgcggcg tatctgtgga tgctgtcggg caaaatgttg ctggtaacgg ctatctggat
     6781 ggcgatcacc atctggggtg gttttgtact ggtggcgcgg gtgtacaaac atatggcgac
     6841 cctgcgtgaa accgaagaca agctgtacac ggattttcaa accgtactcg aagggcgcaa
     6901 agagctgact ctgaaccggg aacgcgccga gtatgtgttt aacaacctct acatccccga
     6961 tgcgcaagag tatcgccatc atattattcg cgcagacacc ttccatctta gcgccgtgaa
     7021 ctggtcaaac atcatgatgc tgggcgctat cggcctggtg ttctggatgg cgaacagtct
     7081 cggttgggct gataccaacg ttgctgcgac ctattcgttg acgctgttat tcctgcgtac
     7141 gccgctgctt tcggcggttg gcgcattgcc gacgctgctg acggcgcagg tggcgtttaa
     7201 caagctgaac aaattcgcgc tcgcgccttt caaagctgag tttccgcgcc cgcaggcttt
     7261 tcccaactgg caaacgctgg agctgcgtaa cgtgacgttt agctatcagg ataacgcgtt
     7321 ttccgttggg ccgattaacc tcaccattaa acgtggcgag ctgctgtttc tgattggtgg
     7381 caacggtagt ggaaaatcga cgctggcgat gttgctgacg ggcttgtacc agccacaaag
     7441 cggcgaaatc ttgctggatg gcaaacctgt cagtggcgaa caaccggaag attatcgcaa
     7501 actgttttcg gcagtgttta ccgatgtctg gctgtttgat caactgctgg ggccggaggg
     7561 taaacccgct aacccgcaac tggttgagaa gtggctggcg cagctgaaaa tggctcataa
     7621 gcttgagtta agcaacgggc gtattgttaa cctgaagtta tcaaaagggc agaaaaaacg
     7681 cgtggcgctg ttgctggcgc tggcagaaga acgcgatatt atcctgctgg atgaatgggc
     7741 ggcggatcag gatccacact tccgtcgtga gttttatcag gtgttgctgc cgctgatgca
     7801 ggagatgggt aaaactattt tcgctatcag tcatgatgat cattacttta tccacgccga
     7861 ccgcctgctg gaaatgcgca atgggcaact tagcgagctg acgggcgaag agcgcgatgc
     7921 cgcttcgcgt gatgccgttg cccggacggc ataatattac gacgctgacc tggcggactg
     7981 ctgccgtcag gtcaatatgc taaaaatccc ttcatttcta ataaccctat aattaatcaa
     8041 cgaaattata atgtttctaa aattagaata taatttataa acattattta aatgttgtta
     8101 cttaagtgtt accgttgatg ccgcgcaaat ctcacctgca ataaagcgac taaaagtaag
     8161 gcattaacaa gatgaaaaaa gtgactgcca tgctcttctc gatggccgtg gggcttaatg
     8221 ccgtttcgat ggcggcaaaa gcgaaagcgt ctgaggagca ggaaactgat gtactgttga
     8281 ttggcggcgg cattatgagc gccacgttgg ggacctattt acgcgagctg gagcctgaat
     8341 ggtcgatgac catggtggag cgcctggagg gtgtcgcgca ggagagttcg aacggctgga
     8401 ataacgccgg aaccgggcat tctgcactga tggaactgaa ctacaccccg caaaacgccg
     8461 atggcagcat cagtattgaa aaagcagtcg ccattaacga agcatttcag atttcccgcc
     8521 agttctgggc gcaccaggtt gagcgcggcg tgctgcgtac tccgcgttca tttatcaata
     8581 ccgttccgca tatgagcttt gtctggggcg aggataacgt taatttcctg cgcgcccgtt
     8641 acgccgcgtt gcaacaaagc tcgctgtttc gcggtatgcg ttactctgaa gatcacgcgc
     8701 agatcaaaga gtgggcaccg ttagtgatgg aagggcgcga tccgcaacag aaagtggcag
     8761 ccacgcgtac ggaaattggt accgatgtga actacggcga gatcacccgc cagttaattg
     8821 cttccttgca ga
//
LOCUS       DAMZET010000170         8814 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.170, whole genome shotgun sequence.
ACCESSION   DAMZET010000170 DAMZET010000000
VERSION     DAMZET010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.170"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1255
                     /gene="murJ"
                     /locus_tag="POB34_003950"
     CDS             <1..1255
                     /gene="murJ"
                     /locus_tag="POB34_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDI5920810.1"
                     /translation="LAVVTVAGMLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYI
                     LLISLASLVGAILNTWNRFSIPAFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVG
                     GVLQLVYQLPHLKKIGMLVLPRINFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFA
                     SFLASGSVSWMYYADRLMEFPSGVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLR
                     LCFLLALPSAVALGILSGPLTVSLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVL
                     APGFYSRQDIKTPVKIAIVTLILTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQ
                     LRKQKIFTPQPGWMAFLLRLVVAVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVL
                     AGIAAYFAALAVLGFKVKEFARRTV"
     gene            complement(1295..1711)
                     /gene="flgN"
                     /locus_tag="POB34_003951"
     CDS             complement(1295..1711)
                     /gene="flgN"
                     /locus_tag="POB34_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HDI5920811.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSADISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(1716..2009)
                     /gene="flgM"
                     /locus_tag="POB34_003952"
     CDS             complement(1716..2009)
                     /gene="flgM"
                     /locus_tag="POB34_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HDI5920812.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            complement(2085..2744)
                     /gene="flgA"
                     /locus_tag="POB34_003953"
     CDS             complement(2085..2744)
                     /gene="flgA"
                     /locus_tag="POB34_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HDI5920813.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLQTFFSAQLAGISDEVR
                     VSIRTAPSLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            2899..3315
                     /gene="flgB"
                     /locus_tag="POB34_003954"
     CDS             2899..3315
                     /gene="flgB"
                     /locus_tag="POB34_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDI5920814.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            3319..3723
                     /gene="flgC"
                     /locus_tag="POB34_003955"
     CDS             3319..3723
                     /gene="flgC"
                     /locus_tag="POB34_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDI5920815.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            3735..4430
                     /gene="flgD"
                     /locus_tag="POB34_003956"
     CDS             3735..4430
                     /gene="flgD"
                     /locus_tag="POB34_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HDI5920816.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            4455..5660
                     /gene="flgE"
                     /locus_tag="POB34_003957"
     CDS             4455..5660
                     /gene="flgE"
                     /locus_tag="POB34_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HDI5920817.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            5680..6435
                     /gene="flgF"
                     /locus_tag="POB34_003958"
     CDS             5680..6435
                     /gene="flgF"
                     /locus_tag="POB34_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HDI5920818.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            6607..7389
                     /gene="flgG"
                     /locus_tag="POB34_003959"
     CDS             6607..7389
                     /gene="flgG"
                     /locus_tag="POB34_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HDI5920819.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEIDSKAVSTTDQMLQKLTQL"
     gene            7442..8140
                     /gene="flgH"
                     /locus_tag="POB34_003960"
     CDS             7442..8140
                     /gene="flgH"
                     /locus_tag="POB34_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HDI5920820.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            8152..>8814
                     /gene="flgI"
                     /locus_tag="POB34_003961"
     CDS             8152..>8814
                     /gene="flgI"
                     /locus_tag="POB34_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HDI5920821.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTI"
BASE COUNT         2026 a         2301 c         2396 g         2091 t
ORIGIN      
        1 gctggcggtt gtgacggtcg ctggcatgct cgccgcaccg tgggtgatca tggtgaccgc
       61 gccaggcttc gctgacacag ctgacaaatt tgccctgacc agccagctac taaagattac
      121 ctttccctat atcttgctga tctccctggc gtcgctggtg ggagcgattc tgaatacgtg
      181 gaaccgcttc tcgattccgg cgtttgcgcc aacactgctt aacatcagca tgattggttt
      241 cgcgctgttt gccgcaccgt actttaaccc accggtgctg gcgctggcgt gggctgttac
      301 ggtcggcggc gtcctgcagc tggtgtatca gctaccgcac ctgaagaaga tcggcatgct
      361 ggtcctgccg cgcattaact tccacgatgc cggagcaatg cgcgtggtga aacagatggg
      421 accggcgatc cttggcgtct ctgtgagcca gatctcctta atcatcaaca ccatttttgc
      481 ctcgtttctt gcttccggtt cggtgtcttg gatgtattac gccgaccgct taatggagtt
      541 tccgtccggt gtgctgggcg tggcgcttgg caccattttg ctgccgtcgc tgtcgaaaag
      601 ttttgccagt ggcaatcatg atgaatacaa ccgtttgatg gactgggggc tgcgtctttg
      661 tttcctgttg gcgctgccga gtgcggttgc gttgggcatt ctttcaggtc cgttgaccgt
      721 ttcgctgttc cagtacggta aatttaccgc gtttgatgcg ctgatgaccc agcgggcgtt
      781 aattgcctac tcggtgggtt tgattggcct gattgtagtg aaagtgttgg ctcctggctt
      841 ttattcccgc caggacatta aaacgccagt gaaaattgcc atcgttacgc tgattttaac
      901 gcaattgatg aacctggcgt ttatcggtcc gttgaaacat gccgggctgt cactttctat
      961 tggtctggcg gcgtgtctga atgcttcgct gctttactgg cagttgcgta agcagaaaat
     1021 ctttaccccg caacctggct ggatggcgtt tctgttgcgt ctggtggtgg cggtactggt
     1081 gatgtctggc gtgcttttag gtatgttaca tatcatgccg gagtggtcat tgggtaccat
     1141 gccctggcgc ttgctgcgtt taatggcggt cgtgctggcg gggattgccg cgtacttcgc
     1201 tgcactggcg gtactgggct tcaaagttaa agaatttgcc cgccggacgg tgtaacaatg
     1261 cattccggcc tgcattgcag gccggagata atcttcagat cgaaatcttt ttactgccgc
     1321 gatgggttgt tgaggtctga ccgttcgccc catatagcgt cggttcctga tgcggtttca
     1381 acatttccag cgcctgttga ttgcgctcaa tctgtccttc cagtaaccag ccgttatgtt
     1441 gattcatttg gcgtagttgc tgcgttttca cagtaatttc ctgccagcgt tgactaatat
     1501 cagcgctatt tgctgtattg ggttctttcc tgcgtaactg ttcgaggtaa tccagcgtcg
     1561 ccagcagtga gcttttttgt tctgtaatcc attgcaactg gctgccgttg atctgcccca
     1621 tagagagatg ttgctgttct tgatccatta ccgttttgag atcgttaagc acagcggaca
     1681 tctggtcgag gatctctgca agacgtgtca tacgatcagt tactctgcaa gtcttgctgc
     1741 gcttcgttga tcagcgcatc ggcaattttg ccggtgtcca tttttagttc accgttacga
     1801 atcgccagtt ttaacgcttc gacacgttca agattgatat cactgctgcc gggttgcatc
     1861 agttttgctt gcgcgtcgct taacgtcaca ctggtgctgg tggaggcggt tgtttttgcc
     1921 gcccggctgt tcgttaccgg cgcgtcagtg gtttcgcgcg gttgaacggt gcttacaggc
     1981 ttcagaggcg aagtgcgatc aatactcatg gtttattcct cattgagggc gcttttatca
     2041 tgtgttgctt atttatcggc aagggacggg taatctttaa cagcttacag gtttataaga
     2101 atattcccat ctgcatcaac aacgccgctg actacctgtc ccgataccat gcgcacccgc
     2161 gcattctgtg cgacggctgc attgttcagc gcctgacctt ctgcgttggc gctaaaccca
     2221 tcaccgctgg cgatcacatt gacgcgttgt cccgctttta cccgccatgc ctggcgaaac
     2281 tgggttaact ggataggttg atcgggtgat agatcgcgca ggctaatggc atcaacaagt
     2341 tgattgatat ccagcaccgt acgcggtggc agggtatcca gccgtccgcg tttcagtttg
     2401 acattgccag cttccagctt tcctccccgc acaatgggca tcgcggcaac cacataattt
     2461 cctgtggcct gtacattaac ctgtaaatat cgtttgtcgt taccgcagcg agccaacaca
     2521 ttcacattgc cccacaggcg ggaattattg ctcatcgaaa gcaatggctg ctcgcacggc
     2581 ggcagtagac tgggcgctgt acgaatagaa acacgaacct catcacttat ccctgcgagc
     2641 tgggcgctaa aaaaggtttg caattgcgat gtgagattac tcgccgcact taacggactg
     2701 aacagtatcg cgatgatcgc cacgctacgt tttattgcca gcattttcgc ccccagccat
     2761 ttctacaacg tgaattgtac ctgtccgcaa tggtcatcaa cggcataaat agcgacccat
     2821 tttgcgttta ttccgccgat aacgcgcgcg taaaggcatt taagctgatg gcagaatttt
     2881 gatacctgcg gaggagatat gctcgataag ctcgacgccg ccttacgttt tcaacaagag
     2941 gcgctcaatc tgcgcgcaca gcgtcaggaa gtgctggcag caaacatcgc caatgctgat
     3001 acccctggtt atcaggcgcg cgatatcgat tttgccagtg aacttaaaaa agtcatgcaa
     3061 cgtggacggg atgcaaccag tgtggttgca ctgacgatga cctcaacgca acacattccg
     3121 gcgcaggcgc tgacgcctcc taccgcagaa ctgcaatacc gtattccgga ccagccttcg
     3181 cttgacggta ataccgtcga tatggatcgc gaacgcaccc agtttgccga taacagcctg
     3241 caataccaga tgagccttag cgcgttgagc gggcaaatca aaggcatgat gaacgtttta
     3301 cagagcggaa attaacggat ggcactgctg aatatttttg atatcgccgg gtcggcgtta
     3361 actgcccagt cccagcgcct gaacgtggcg gccagtaatc tggcgaatgc tgatagcgtg
     3421 accggtcctg atggacagcc atatcgggca aaacaggtgg tattccaggt taacgctgca
     3481 ccaggtgctg cgacaggcgg cgtaaaggtt gccgatgtta tagaaagtca ggccccggac
     3541 aaactggttt atgaaccggg taatccgctg gcggatgcaa agggctacgt gaaaatgcca
     3601 aacgttgatg tcgtcgggga gatggttaac accatgtcgg cgtcacgcag ctatcaggcc
     3661 aatgttgaag tgctcaacac ggtgaaaagc atgatgctga aaacccttac gctcggtcaa
     3721 taaaggagaa agctatgtcc attgcggtaa ccaccaccga tccgacaaat accggcgtca
     3781 gtaccaccag cagtagttcg ctcacgggca gcaacgccgc agatttacaa agcagttttc
     3841 tgactttgct ggtggcgcag ctgaaaaacc aggacccgac caatccaatg gaaaacaacg
     3901 agctgacgtc gcaattggca caaatcagca cggtcagcgg gattgaaaaa ctcaatacca
     3961 cgctcggatc tatttccgga cagattgata acagccagtc gttacaggcc agtaacctga
     4021 ttggtcacgg cgtgatgatc cccggcacca ctgttcttgc gggaaccggc agtgaagaag
     4081 gggctgtgac cacgaccacg ccgtttggtg ttgagctgca acaggcggca gacaaagtta
     4141 cggccaccat caccgataaa aatggcgcgg ttgtgcgcac cattgatatt ggtgaactga
     4201 ccgccggagt ccacagtttc acctgggacg gtacgttgac tgacggcagc actgcgccga
     4261 acggttctta caacgtagcg attagcgcca gtaacggtgg tacacaactg gttgcccagc
     4321 cgctgcagtt tgctctggtg cagggtgtga tccgcggcaa caacggtaat acgctggatc
     4381 tcggcactta cggcaccacc accctcgacg aagtacggca gataatttaa gccttcacat
     4441 ttcaggagtc agtcatggcc ttttctcaag cggttagcgg attaaacgct gccgccacca
     4501 acctcgatgt tattggcaac aatatcgcca actccgccac atacggcttt aaatcaggca
     4561 cggcctcttt tgccgatatg tttgccggtt cgaaagtggg actgggggta aaagttgccg
     4621 gtatcactca ggactttacc gatggcacga ccaccaacac cgggcgtggt ctggacgttg
     4681 ctatcagcca gaacggtttt ttccgtctgg tagacagcaa cggttcggtg ttctacagcc
     4741 gtaacggaca atttaagctg gatgaaaacc gtaacctggt gaatatgcaa ggtttacagc
     4801 tgaccggtta cccggcaacc ggtacgccgc cgactattca gcaaggggcg aatccgacta
     4861 acatttcgat cccgaatacc ctgatggcag cgaaaactac caccacggcg tcgatgcaga
     4921 tcaacctgaa ttccagtgat ccgcttccta ctgttacgcc attcagcgcc agcaatgcgg
     4981 atagctataa caaaaaaggt tcggtgactg ttttcgacag tcagggtaat gctcatgaca
     5041 tgagcgtcta ctttgtgaag accggggata ataactggca ggtctacacc caggatagca
     5101 gtgatccaac aggtacagcc gagcctgcaa tgaagctggt gtttaatgcc aatggcgttc
     5161 tgacctcaaa tccaacagag aatattacca ctggcgcaat taacggcgca gatcccgcca
     5221 cgtttagcct gagcttcctc aactccatgc agcaaaatac cggcgctaac aacattgtgg
     5281 caaccaccca gaacggctac aaaccgggcg atctggtgag ttatcaaatc aatgatgacg
     5341 gtacggttgt cggcaactat tccaacgaac aaacccaact gctggggcag attgtactgg
     5401 cgaactttgc caacaacgaa ggtctggcat ccgaaggcga caacgtctgg tctgcgacgc
     5461 aatcttctgg cgtggcgctg ttggggacag ccgggacggg caactttggt accctgacca
     5521 acggtgcgct ggaagcgtcc aacgtcgatc tcagtaaaga actggtcaat atgatcgttg
     5581 cccagcgtaa ctatcagtct aacgcccaga ccattaaaac ccaggaccag atcctcaaca
     5641 cgctggttaa cttacgctaa tcgctgacgg gatagctcaa tggatcacgc aatttatacc
     5701 gcgatgggag cagccagcca gacgctgaat caacaggcgg taaccgccag taatctggcc
     5761 aatgcctcta cgcccggttt tcgtgcgcag ttaaacgcct tgcgcgcagt gccggtggaa
     5821 gggctttcac tgcctacgcg cacgctggta acggcgtcaa caccgggtgc agacatgacg
     5881 cccggcaaga tggattacac ctcgcgcccg ctggacgttg cgttgcagca ggatggttgg
     5941 ctggccgtgc agaccgctga cggcagcgaa gggtatacgc gtaatggcag cattcaggtt
     6001 gatcccaccg ggcaactgac aattcagggg catccggtga taggcgaggc ggggccaatt
     6061 gctgtgccgg aaggggcgga aatcacgata gctgccgatg gcacaatctc ggcgctgaat
     6121 ccgggcgatc cggcaaatac ggttgcgcca gtggggcgtc tgaagctggt gaaagccacg
     6181 ggcagcgaag tgcagcgcgg tgacgacggc atttttcgtt taagcgcaga aacccaggcc
     6241 acgcgtgggc ctgtactgca ggcagatcca accttgcgtg tgatgtcggg tgttctggaa
     6301 ggcagtaacg tcaatgccgt tgcggcaatg agcgacatga ttgccagcgc gcggcgtttt
     6361 gaaatgcaga tgaaggtgat cagcagcgtc gatgataacg caggccgtgc caaccaactg
     6421 ctgtcgatga gttaattgaa aggatacatg acaagtataa gttgcccgat gcgcaagttt
     6481 atcgggtcta tgggggcaat cgcaatttat cgattttgcg agcacttgta ggccggataa
     6541 ggcgtttacg ccgcatccgg caagaagaca tatgcacttt gtcactaatc cactacagga
     6601 cattttatga tcagttcatt atggatcgcc aaaacgggcc ttgacgccca gcaaaccaat
     6661 atggacgtca ttgccaacaa cctggcaaac gtcagtacta acggttttaa gcgtcagcgc
     6721 gcggtgtttg aagatctgct ttatcaaacc attcgtcagc cgggggcaca gtcttccgaa
     6781 caaaccacct taccctccgg attacaaatc ggcacggggg tacgcccggt cgccactgaa
     6841 cgcttacaca gccagggcaa cctgtcgcag accaacaaca gcaaagatgt cgcgattaaa
     6901 gggcagggct ttttccaagt gatgttgcca gatggctcat cagcctatac ccgtgacggc
     6961 tctttccagg tggatcagaa cgggcagttg gtgacggctg gtggttttca ggtgcagcca
     7021 gcgatcacca ttccggcgaa tgcgttaagt atcaccatcg gtcgtgatgg cgtggtcagc
     7081 gtaacccaac aaggccaggc agctccggtc caggttgggc agctcaatct caccaccttt
     7141 atgaatgata ccggactgga gagcattggc gaaaacctct acaccgaaac gcaatcctcc
     7201 ggtgcaccga acgaaagcac gccgggcctg aacggtgcgg gactgctgta tcaagggtat
     7261 gttgaaacgt ctaacgtcaa cgtggcggaa gaactggtca atatgattca ggtgcaacgc
     7321 gcttacgaaa tcgacagtaa agcggtgtcc accaccgatc agatgctgca aaaactgacg
     7381 caactctaag gcataaccgg tggcaggttc accggtttat tgatttttga agatgatagc
     7441 catgcaaaaa aacgctgcgc atacttatgc catttccagc ttgttggtgc tttcactaac
     7501 cggctgcgcc tggataccct ccacgccgct ggtacaggga gcgaccagtg cacaaccggt
     7561 tcccggtccg actcccgtcg ccaacggttc tattttccag tctgctcagc cgattaacta
     7621 tggctatcaa ccgctgtttg aagatcgtcg accacgcaat attggcgata cgctgaccat
     7681 cgtgttgcag gagaacgtca gcgccagcaa aagctcctct gcgaatgcca gccgtgacgg
     7741 taaaactaat tttggctttg atactgtgcc gcgctatttg caggggctgt ttggtaacgc
     7801 tcgtgccgat gtcgaagcct ccggtggtaa cacgttcaac ggaaaaggcg gggccaatgc
     7861 cagcaatacc tttagcggca cgttgacggt gacggttgac caggtactgg tcaacggcaa
     7921 cctgcatgtg gtgggtgaaa aacagattgc cattaatcag ggtaccgaat ttattcgctt
     7981 ctctggcgtg gttaatccac gcaccatcag cggcagcaat accgtaccgt ctactcaggt
     8041 ggcggatgcg cgcattgaat acgtaggcaa tggctacatt aacgaagcgc aaaatatggg
     8101 ctggttgcaa cgtttcttcc ttaacctgtc gccaatgtaa gtgaggctgt ggtgattaaa
     8161 tttctctctg cattaattct tctactggtt acgacggcgg ctcaggctga gcgtattcgc
     8221 gatctcacca gtgttcaggg ggtaaggcaa aactcactga ttggctatgg cctggtggtg
     8281 gggctggatg gaaccggtga ccagacgacc cagacgccgt ttaccacaca aacgcttaat
     8341 aacatgctct cacagctggg aattaccgtt ccgacgggca ccaatatgca gctaaaaaac
     8401 gtcgctgcgg taatggtgac agcgtcactt cctccgtttg gacgtcaggg gcaaaccatc
     8461 gatgtggtgg tttcctccat gggtaatgcc aaaagcttgc gtggaggtac gttgttgatg
     8521 acaccgctta agggcgttga cagtcaggtg tatgcgctgg cgcagggcaa tattctggtt
     8581 ggcggcgcag gagcctccgc tggcggtagc agtgttcagg tgaaccaact gaacggtgga
     8641 cggatcacca atggtgcggt tattgaacgt gaattgccca gccagtttgg cgtcgggaat
     8701 acccttaatt tgcaacttaa cgacgaagat ttcagcatgg cgcagcaaat cgctgacacc
     8761 atcaaccgcg tgcgtggata tggcagcgcc accgcgttag atgcgcggac tatt
//
LOCUS       DAMZET010000171         8525 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.171, whole genome shotgun sequence.
ACCESSION   DAMZET010000171 DAMZET010000000
VERSION     DAMZET010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.171"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..527)
                     /locus_tag="POB34_003962"
     CDS             complement(<1..527)
                     /locus_tag="POB34_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HDI5920822.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKPDLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHG"
     gene            complement(533..1660)
                     /gene="efeO"
                     /locus_tag="POB34_003963"
     CDS             complement(533..1660)
                     /gene="efeO"
                     /locus_tag="POB34_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HDI5920823.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAANVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(1718..2548)
                     /locus_tag="POB34_003964"
     CDS             complement(1718..2548)
                     /locus_tag="POB34_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HDI5920824.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            2584..2886
                     /locus_tag="POB34_003965"
     CDS             2584..2886
                     /locus_tag="POB34_003965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920825.1"
                     /translation="MQRKVNRSDSDYHYGKEKYKRNVYKMMLECKQREVMPRDINDYS
                     RVERWDCLQRTMCSCRMPRKRRIQPTKLSRLNASELPCKTDKRVYQAISSIRDYQI"
     gene            complement(3091..4599)
                     /gene="putP"
                     /locus_tag="POB34_003966"
     CDS             complement(3091..4599)
                     /gene="putP"
                     /locus_tag="POB34_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDI5920826.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            5021..>8525
                     /gene="putA"
                     /locus_tag="POB34_003967"
     CDS             5021..>8525
                     /gene="putA"
                     /locus_tag="POB34_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HDI5920827.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQ"
BASE COUNT         2081 a         2361 c         2194 g         1889 t
ORIGIN      
        1 tcaccatgac ataacgccgc atccagcgag tcgttgggaa aacgcgtcat cttctgcaac
       61 tttttcggca tctgtggcgc aaggccaaag cgctcatcaa acaatgagtg acccaccgat
      121 aacgtgatgg tgagattatc gggcgcaatg tagccgccaa gaatgccgga atcgagtggt
      181 ggcaggcgcg gatttggcgt ttctggtgcc gctccgccct gagtcagaaa agcaaaacgc
      241 tgagtcaaca agcgaaacaa ccgttcaaga tcgggtttat cgctggcaag cacatcaaac
      301 gccaccagca tcattgcggc ctgttgtggc gtcaggatcc ctgcctgatg ctcaccataa
      361 aacggctgtt tctcattgcg tgcatccggt gaaagcgtac ccggcgcact ttgcgttttt
      421 tgtgcatgag cgaccggaca acttcccgcc agcgccagcg caccaatgcc tttcagtaaa
      481 cgtcggcgtg acggttcatt cacgccgttt ttatcttcat actgcataat gcttaatcca
      541 atcccagcac accgcgaagt tgcgccagat cttccgccag cgcagtaatc ggtcctttca
      601 gtgcattccg gtcggcatcg gtcaatttgt cgtaggtttc aaaaccgtct ttagtacggt
      661 atttcgccag aatggtatcg acctttttaa agttggcatc gacttttgcc aacagttccg
      721 ggttggcttt ttgcagttgt ggacgcagca gatcgacaat tttctgcgag ccttcaacgt
      781 tagcctggaa atcccacaga tcggtgtggc tgtagcgatc ttcttcaccg ctgattttgc
      841 tggctgccac ttcctcaatc agtccggctg caccgccgac cacttttgaa ggtgggaaag
      901 ccagttcact gatgcgtttt tgcaaatcga ccacatcggt ataaagctgg tcagcgtact
      961 gatccatgcc tttggtggtg ttgtcgccaa acaatgcttt ttccagacgg tggaaaccgg
     1021 tgaatttcgg atcggcggct ttttgctcgt aatcatcttc acgggcgtca atgctgccat
     1081 ccagatcgga gaacagttca gcaatcggtt cgatgcgctc atagtgctgg cgcgtcggcg
     1141 catacagtgc tttcgctttt tcgatatcgc ctgctttaat agcgtcagta aaggctttcg
     1201 tgtcggtcac cagctgcgtg gtttccgcca tgacatacgc tttatatgca gtaattgcac
     1261 caccaagact taacagcgca tcactttgcg ccgcatccgc cgttgcctca cctttgacga
     1321 tcaacttccc tttcgggtta gtcagcagac cgcaggtcat atcgtattcg ccaggctgta
     1381 aattcgccgt cattttctgg ctaaagccag gggcgatatt ttcccgctct tccaccacca
     1441 tcacgccttt gaggatctcc cactccagcg ccttctggct gtggttctga ataatgaact
     1501 gtgttttccc ggcgttaacc gtaatggtca tcggttcgca ctgcttatcg gtcacggtca
     1561 ccttgacctg cggcacatta gcggcgttag ccataaaagc agaagagaac agcgcagcca
     1621 cgctcaactg caatgcgtta cggcggaagt taatggtcat gacgagacta tccctttaaa
     1681 gtatgttgta actaagcaag agtttgcgtc gtatttgcta cgcggagcga gacgctgtcg
     1741 cccctgcgcg tggtggcagg gcaaatgcca ccagcgccgg gatgagataa ataaaccaga
     1801 cggcgacttc gctgacgctc ggtgcttcct gatagccaaa aatcccttcc atcagcgtgc
     1861 caaacagcga gtgagttgag aggaccgcgc tcatatcgaa ggcgatttcc tgaaagtggt
     1921 tccacaatcc ggcttcatga aatgcgcgaa tggcaccagc cgccagccct gcggcgacga
     1981 agagaataaa caggctggtc catttaaaaa atgcaccaag attgaggcga ataccgcccc
     2041 agtagagcag gaagccgagc accacggcag tagcaagacc gagcattgca cccagcggcg
     2101 gccagatccc gacatcttgt tgaaatgccg ccagcaggaa aaagaccgac tccagccctt
     2161 cccttgcaac ggcaaaaaag accatcatca ccagcgccca gccatgatga tttccacgct
     2221 gcaatgcgct atcgactgcc tgttccagtt gcactttgac gttgcgcgac actttgcgca
     2281 tccagaaaac catccaggta aggatcacca cggcgatcac cgccacgata ccttcaaaca
     2341 gttcctgttc tttttgcgga aattcgccgg tggtttcgtt aatgaagata cccaggccca
     2401 ggcacaacgc agcggcaagc aacacgccaa tccacatcac accaatccat cggcctcgct
     2461 gggtacgctt aagatagctg gcaatcaaac tgacaatcag cgcggcttca agtccttcgc
     2521 gcaacataat gagaaacgga acaaacatgc cacccaccct taaacgtttt tctgagtcaa
     2581 tctatgcaaa gaaaagtaaa tcgcagtgat agcgattatc attacggcaa agaaaaatac
     2641 aagcggaatg tttataaaat gatgttggaa tgtaaacaaa gagaggtaat gccaagggat
     2701 ataaacgatt attccagggt agaaagatgg gattgtttgc aaagaaccat gtgttcttgc
     2761 cggatgccgc gtaaacgccg tatccagccg acaaaactgt ccagactcaa tgcatcagag
     2821 cttccatgta aaactgataa gcgggtgtat caagcaattt cgtccatccg cgattatcaa
     2881 atctaacttt ttgcccggaa cataataaca gctcgggtgt tggtggcgtc aaaatcgctg
     2941 aagcgttccc cgaaggccgg gacagcaggg ggcggcggcg attggccgcc ccctgcgcgc
     3001 tccctgcacc attcaattta tatggcgagg cacattaagg gagcgcaacc cgctgctgaa
     3061 gccaaacaaa accgccgcag gctaagtccc ttagctttcc tgcaaccgtg acggcggagc
     3121 cgaatgatag tgcgcatcgg cctcggcaaa gcgtttttgc atcgccgctg acggcgcttt
     3181 acccagcaaa ctaaacacta caatcccaat actgccgaag ataaagcccg gaataatttc
     3241 gtagagtccc agccagccga actgcttcca gacgataacc gtcagcgcac cgatgatcat
     3301 ccccgccagc gcaccgttgc gcgtcatgcg tgaccacatc accgagaaca gcaccaccgg
     3361 accaaacgcc gcgccaaagc ctgcccacgc gtagctcact aagcccagca cgcggttttc
     3421 cgggtttgcc gccagcgcaa tcgccaccag cgccaccacc agcaccatca cacgccctac
     3481 ccacaccagc tctttctggc tggcatgttt acgcagaaac gctttgtata aatcttcggt
     3541 aatcgcactg gagcacacca gcagctggca gcttaacgtt gacattaccg ccgccagaat
     3601 tgccgacagc agaatcccgg caatccacgg gttaaacaga atttgcgcca gttcgataaa
     3661 cacacgttca gcgttctggt ttaccgcacc tgctaccgcc gggtgctcat taaagtaagc
     3721 aatcccaaag aagccgacag ccaccgcccc tgccaggcag aggatcatcc aggtcatact
     3781 gatacgacgc gcatggacaa tgctgtggtg agaatccgcc gccataaaac gcgccagaat
     3841 atgaggctga ccgaagtaac ccagccccca acccatcaat gagataatgg caacaaagtt
     3901 cagccctttg agcatatcca cgttttcgat gctcttttgt ttgatcactt ccagcgagtc
     3961 accaaagcca ccgacactga taatgacgat aaccggcgtc aggatcaggg caaaaatcat
     4021 caggctggcc tgtacagtgt cagtccagct caccgcgagg aaaccgccaa taaaggtgta
     4081 aaggatcgtc gccgcagcgc ccgcccacag agccgtttcg tagctcatgc caaaggtact
     4141 ttcaaacaga cgcgcgcctg ccacaatgcc cgaagcgcaa taaatggtga agaacagcaa
     4201 aataaccagc gcagagataa tgcgcaaaat gcggctttta tcttcaaagc gcccggtgaa
     4261 ataatccggc aatgttaagg cgttattgtt gtattcggta tgcacacgca accgcccggc
     4321 caccagcttc cagttgatcc acgcgcctaa tgtcaggcca atggcgatcc agctttcgga
     4381 aatcccggaa agaaaaaccg cgcccggcaa ccccattaac agccagccgc tcatatccga
     4441 cgcacccgcc gataatgccg tcacgaatgg cccaagacta cgaccgccca gaatatagtc
     4501 gtcaaagttt ttcgttgatc gccaagcgat aaacccaatc aatatcatgc caaagatata
     4561 gacacaaaat gtcaccaaca tcggtgtgct aatagccatc taaagtctcc aaaaaattat
     4621 tatcggcaat gtcgaaactt gccgttatat ctgccaccgg aacggggtaa cagagtttat
     4681 gttttaccag ggcgaccgta tcctgccgga agcgctggtt attcacaatc gatttaacac
     4741 accatttaca ttaaatttta gtgctcagcg acactatttt tcatcaggtt gcactctctc
     4801 acattttttg cggttgcacc tttcaaaaat gttaactgcc gcagagaaaa agtctgagtt
     4861 attttttaat ccctgtcata tcgatttctt ttattaacat ttcattcatt tttaagcttg
     4921 ctacgcaggt cacatttaac atggttgcac aaagttgcaa catcatggat atttcacgat
     4981 aacgttaagt tgcacctttc agaacaacag gagtaatggc atgggaacca ccaccatggg
     5041 ggttaagctg gacgacgcga cgcgtgagcg tattaagtct gccgcgacac gtatcgatcg
     5101 cacaccacac tggttaatta agcaggcgat tttttcttat cttgaacaac tggaaaacag
     5161 cgatactctg ccggagctac ctgcgctgct ttctggcgcg gccaatgaga gcgatgaagc
     5221 accgactccg gcagaggaac cacaccagcc attcctcgac tttgccgagc aaatattgcc
     5281 ccagtcggtt tcccgcgccg cgatcacagc ggcctatcgc cgcccggaaa ccgaagcggt
     5341 ttctatgctg ctggaacaag cccgcctgcc gcagccagtt gctgaacagg cgcacaaact
     5401 ggcgtatcaa ctggccgata aactgcgtaa tcaaaaaaat gccagtggtc gcgcaggcat
     5461 ggttcagggg ttattgcagg aattttcgct gtcatcgcag gaaggtgtgg cgctgatgtg
     5521 tctggcagaa gcgttgttgc gtattcccga caaagccacc cgggatgcat taattcgcga
     5581 caaaatcagc aacggtaact ggcagtcaca cattggtcgt agcccgtccc tgtttgttaa
     5641 tgccgctacc tgggggctgc tgtttaccgg caaactggtt tccacccata acgaagccag
     5701 cctctcccgc tcgctgaacc gcattatcgg taagagcggt gaaccgctga tccgcaaagg
     5761 tgtggatatg gcgatgcgcc tgatgggcga gcagttcgtc acgggtgaaa ccattgcaga
     5821 agcgttagcc aatgcccgca agctggaaga gaaaggtttc cgttactctt acgatatgct
     5881 tggcgaagct gcactgactg ccgcagatgc acaggcgtat atggtttcct atcagcaggc
     5941 gattcacgcc attggtaaag cgtccaatgg tcgtggcatc tatgaagggc cgggcatttc
     6001 aatcaaactg tcggcgctgc atccgcgtta tagccgcgcc cagtatgacc gggtaatgga
     6061 agagctttac ccgcgtctga aatcactcac cctgctggcg cgtcagtacg atattggtat
     6121 taacatcgat gccgaagagg ccgatcgtct ggagatctcc ctcgatctgc tggaaaaact
     6181 ctgtttcgag ccggaactgg caggctggaa cggcatcggt tttgttattc aggcttatca
     6241 aaaacgctgc ccgttggtga tcgattacct gattgatctc gccacccgca gccgtcgccg
     6301 tctgatgatt cgcctggtga aaggcgcgta ctgggatagt gaaatcaagc gtgcgcagat
     6361 ggacggcctt gaaggttatc cggtttatac ccgcaaggtg tataccgacg tttcatatct
     6421 cgcctgtgcg aaaaagctgc tggcggtgcc gaatctaatc tacccgcagt tcgcgacgca
     6481 caacgcccat acgctggcag cgatttatca actggcaggt caaaactact atccgggtca
     6541 gtacgagttc cagtgcctgc atggtatggg cgagccactg tatgagcagg tcaccgggaa
     6601 agttgccgac ggcaaactta accgtccgtg tcgtatttat gctccggttg gcacacatga
     6661 aacgctgctg gcgtatctgg tgcgtcgcct gctggaaaac ggtgctaaca cctcgtttgt
     6721 taaccgtatt gccgacacct ctttgccact ggatgaactg gtcgccgatc cggtcactgc
     6781 tgtagaaaaa ctggcgcaac aggaagggca aactggatta ccgcatccga aaattcccct
     6841 gccgcgcgat ctttacggtc acgggcgcga caactcggca gggctggatc tcgctaacga
     6901 acaccggctg gcctcgctct cttctgccct gctcaatagt gcactgcaaa aatggcaggc
     6961 cttgcccatg ctggaacaac cggtagcggc aggtgagatg tcgcccgtta ttaaccctgc
     7021 ggaaccgaaa gatattgtgg gctttgtgcg tgaagccacg ccgcgtgaag tagaacaggc
     7081 gctggaaagt gcggttaata acgcgccaat ctggtttgcc actcctccgg ttgaacgcgc
     7141 ggcgattttg catcgcgctg ccgtgctaat ggaaagccag atgcagcaac tgattggtat
     7201 tctggtgcgt gaagccggaa aaaccttcag taacgccatt gccgaagtgc gcgaagcggt
     7261 cgattttctc cactactatg ccggacaggt gcgggatgat ttcgctaacg aaacccaccg
     7321 tccattaggg cctgtggtgt gtatcagtcc gtggaacttc ccgctggcga ttttcaccgg
     7381 gcagatcgct gccgcactgg cggcaggtaa cagcgtgctg gcaaaaccgg cagaacaaac
     7441 gccgctgatt gccgcgcaag ggatcgccat tttgctggaa gcgggtgtac cccctggcgt
     7501 ggtgcaattg ctgccaggtc agggggaaac cgtgggcgcg caactgacgg gtgatgatcg
     7561 cgtgcgcggg gtgatgttta ccggttcaac cgaagtcgct acgttactgc agcgcaatat
     7621 cgccagccgc ctggacgctc agggtcgccc tattccgctc atcgctgaaa ccggcggcat
     7681 gaacgcgatg attgtcgatt cttcagcact gaccgaacaa gtcgtcgtgg atgtgctggc
     7741 ctcggcgttc gacagtgcgg gtcagcgttg ttcggcactg cgcgtgctgt gcctgcaaga
     7801 tgagattgcc gaccacacgt tgaaaatgct gcgcggtgca atggccgaat gccggatggg
     7861 taatccgggt cgcctgacca ccgatatcgg tccggtgatt gatagcgaag cgaaagccaa
     7921 tattgagcgc catattcaga ccatgcgtag caaaggccgt ccggtgttcc aggcggtgcg
     7981 ggaaaacagc gaagatgccc gtgaatggca aagcggcacc tttgtcgccc cgacgctgat
     8041 cgaactggat gactttgccg aattgcaaaa agaggtcttt ggtccggtgc tgcatgtggt
     8101 gcgttacaac cgtaaccagc taccagagct gatcgagcag attaacgctt ccggctatgg
     8161 tctgacgctt ggtgtccata cgcgtattga tgaaaccatc gcccaggtca ctggctcggc
     8221 ccatgttggt aacctgtatg ttaaccgtaa tatggtgggc gcagtggtgg gtgtgcagcc
     8281 attcggcggc gaagggttgt ccggtaccgg gccgaaagca ggcggtccgc tctatctcta
     8341 ccgtctgctg gcgaatcgcc cggaaagtgc gctggcagtg acgctcgcgc gtcaggatgc
     8401 agagtatccg gtcgatgcac agttgaaagc cgcattgact cagccgctaa atgcactgcg
     8461 ggaatgggca gcaaatcgtc cagaattgca ggcgttatgt acgcaatatg gcgagctggc
     8521 gcagg
//
LOCUS       DAMZET010000172         8499 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.172, whole genome shotgun sequence.
ACCESSION   DAMZET010000172 DAMZET010000000
VERSION     DAMZET010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.172"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            330..986
                     /gene="pphA"
                     /locus_tag="POB34_003968"
     CDS             330..986
                     /gene="pphA"
                     /locus_tag="POB34_003968"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDI5920828.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(987..1178)
                     /locus_tag="POB34_003969"
     CDS             complement(987..1178)
                     /locus_tag="POB34_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HDI5920829.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(1283..1519)
                     /locus_tag="POB34_003970"
     CDS             complement(1283..1519)
                     /locus_tag="POB34_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HDI5920830.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(1637..3076)
                     /gene="rsmF"
                     /locus_tag="POB34_003971"
     CDS             complement(1637..3076)
                     /gene="rsmF"
                     /locus_tag="POB34_003971"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HDI5920831.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAATGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(3156..5789)
                     /gene="yebT"
                     /locus_tag="POB34_003972"
     CDS             complement(3156..5789)
                     /gene="yebT"
                     /locus_tag="POB34_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HDI5920832.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGLVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(5758..7041)
                     /gene="yebS"
                     /locus_tag="POB34_003973"
     CDS             complement(5758..7041)
                     /gene="yebS"
                     /locus_tag="POB34_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HDI5920833.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            7171..7668
                     /gene="msrC"
                     /locus_tag="POB34_003974"
     CDS             7171..7668
                     /gene="msrC"
                     /locus_tag="POB34_003974"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HDI5920834.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            7765..8463
                     /gene="proQ"
                     /locus_tag="POB34_003975"
     CDS             7765..8463
                     /gene="proQ"
                     /locus_tag="POB34_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HDI5920835.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
BASE COUNT         2161 a         2188 c         2131 g         2019 t
ORIGIN      
        1 tttttaacat ttcagctgat gaccaccacg ctttttattg accattttgc acgcaaactg
       61 gaaaacctgg cgtcgtcatc tattcttaaa gggcaaggca actaagcctg cattaatgcc
      121 aacttttagc gcacggctct ctcccaagag ccatttccct ggaccgaata caggaatcgt
      181 gttcggtctc tttttatctg ttaaaagcca gaagcatttc cttcgctgac tttatagtca
      241 accataacac acactctact gtctgagtcc agcgtttttt aacatccttg ttaagattat
      301 gtgatcttta gcgcgggagg aaaatattga tgaaacagcc tgcgcccgtt tatcagagaa
      361 ttgcgggtca tcaatggcga catatctggc tttctggcga tatacacggt tgtcttgagc
      421 agttgcgccg caaattatgg cattgtcgtt ttgatccgtg gcgagattta cttatctcag
      481 taggagacgt tatcgatcgt gggccgcaaa gtttacgttg tctgcagtta ctggaacaac
      541 attgggtttg tgcggtaaga ggcaatcatg aacagatggc gatggatgcg ctggcatccc
      601 agcagatgtc tttgtggttg atgaatggcg gcgactggtt tattgcgctg gcagataatc
      661 aacagaaaca agcgaaaacg gcgctggaaa aatgtcagca tttgcccttt attcttgaag
      721 tacacagtcg taccggcaaa catgttattg ctcatgccga ttatccagat gatgtttatg
      781 aatggcaaaa ggacgttgat ttgcatcagg tcttgtggag ccgctcgcga ttaggtgaac
      841 gccaaaaagg gcagggaatt acaggtgctg atcatttctg gtttggtcat acaccgttgc
      901 gacatcgcgt ggatattggc aacctgcatt atattgatac cggtgctgtc tttgggggcg
      961 aactgactct tgtgcaattg caataattaa aaatcaccat actcctgtgc tggtcgccag
     1021 aaaccatcta taaaatcctc aatcggaaaa caaccgccat ggcggatccg ttgatcgctc
     1081 atagaataaa gacactgctg ttccgtgttg tagacatcca caacaatatc ttcacaaccg
     1141 ccatccaggt agcaaacaaa aagtaccagc gcgaacattt catccccgaa gtgtggtgcc
     1201 gtaccgttaa gtttaggaga gattttacaa cgggggaata accaggacaa ataacccgcc
     1261 atgccgacgg gttctttttg gatcaggcaa gacgcataat ccaggcatca gattgttgat
     1321 cgtaacgggt acggtaaagg acagagtgtt cgccatttag cagggcaggg atactggttt
     1381 cagcatccca ggcatccagt tgcatacata aatcggggtc agatttacaa ggaatggtta
     1441 acgttcgatc acctggcgat tcaccgtgta attcgccgtc gtcgatttca aaagcgccta
     1501 tgcgcacact ggttttcatc ttcgtgctct ccatttagct gccgattgtg gtacgaccag
     1561 tccgatcgca ccatttttca gcctagttga ggcagccgaa tgtgccagtc aaaaagtgcg
     1621 ctttttttgt gcgctgtcag gcgttaccgg taaaaagttt cccatcgcgc accagttcac
     1681 gcggatagct gtttttcaat cgcgaaccaa tccgtttggc taaaccaatc ggttgatgct
     1741 ggaaagtaac caatacatca tccgccactg gcgcggcttg cgggtaaaca tcgcgcccgc
     1801 gataccactc ctccgcttcc tgcggtgtca gctcgaatgc attcatatta tcgggtgagg
     1861 caagagcaat aaccgcttca tgctgccagc gataaccttt gttgtgcgtc tcggcaagtt
     1921 taatccccaa ccgagaaaat cggactttac cgatcagggc ttcaatgccc accgggaaca
     1981 accacagttc tttgtcacgc tgccagaggc gcaggttttc atcccagttt aagccaacac
     2041 ctgtagccgc ctgacgaatt tgtccagctt cgcgatcttt caccgggctg aacggaaaat
     2101 taccgacttt gtatttgggg gcgggtaagg cgggaatcgc ctgagtttta cgcagacgag
     2161 caacgaagaa gccttcgcag tcgtaaattt gtgggaaaac atgcaaaaag ccttcttcgg
     2221 tcagcgcttt gtttgcacca gggaagagat cgccaagtgg taaaaactct actgcgtcgg
     2281 ggtaagtctc tttcagccac aggcaaacgg cttcgttttc ttcctggttt aaggtacagg
     2341 tcgagtaaac cagcgtacca ccaggacgta atgcatgaaa ggcgctgtcg ataagctccc
     2401 gttgtgtagc tgcgatttcc tgattgcttt ctggtgacca gttttttagt gcatcgggat
     2461 ctttacgcac cacgccttcg ccagagcagg gagcgtccag caaaatggca tcgaacattt
     2521 ctggcactgc cgcaccaaac acgcggccat caaaatgtgt gagcgcaaca ttactgatgc
     2581 cacagcggct gatattggca tgtaacactt ttacccgact ggcggaaaac tcattggcaa
     2641 ggattgcccc ttcgttattc atccgcgcgg caatttgcgt cgttttggag cctggcgcag
     2701 ctgcgacatc catcacccgc tgtggtgcat tatcgtcagc aaacaaggcg gcaacgggca
     2761 acattgaact ggcttcctga atataaaaca ggccgcttaa atgctcggcg gtactaccca
     2821 atggcaatgc atcttcattg tcgcgttcaa tccagaaacc ttcttcacac cacggaattg
     2881 gcgtaagcgt ccagccataa ggagcggtta attgcaggaa atcagcaacg gagattttca
     2941 gcgtattaac gcgaatgctg cggcgcaacg ggcgctgaca ggcggcaaga aaatcatcaa
     3001 atgagagcgt cgaaggcatc gcttcgcgca tttgtgtcag aaaggcgtcc gggaaataaa
     3061 cggtgtgttg ggccacgagc atgtaccact ggcaaaaaaa caggcgcgca gtttagcata
     3121 aacgctccgg cgcaggcacg ccggagcagt gggcattatt tgggaagcgc agttccccat
     3181 tcacgccact ctttcggttc actttcctgc aacaggaagt gtttgccttc ctgggctttc
     3241 ggtgccagtg gcgtacccgg aggcgtggcg aaggcgatgc cgccacggat aaactggtta
     3301 aaggtgccgg ttttcactac gccgcccgtc agaccaaagt ccagactgta acccgatgcc
     3361 aaccagaaga cggaattgtt acgcaccagg tgttgatagc gtttactgat gcgcatcgca
     3421 atcatcacgc gatctgacaa tgtccccagc gtcattcctg taaccgtacc gacttccaga
     3481 ccacggaaca gcacaggcgt accaatacct aacgaaccgg cttccggcgc ttcaacaata
     3541 atgcttaagc catccaggta acgcgaatca gtaatggtgg cctcttgtaa ttcaaagtcg
     3601 cggcgaggat tgccccggcc tggttcgacg ttgatatacg gctggaggat agtatctaga
     3661 tgctcaacgc ccgcggctga aatttgcggt gtgaccaccg agaagcgcgt accgccgcgg
     3721 gcgaaggtct ggacatactc cggatagagc accgcctttg cctgtacttc attgcgcgcg
     3781 gtaatcagat ccagcgtctg gatttgcccg atatcaatac cgagatagcg aattggcatc
     3841 ccgaccgcca gttttccggc atcgaaagcg tgaagcgtaa tctgcccacc aaccgcacgg
     3901 gccgctgttt cggaagcata cagaattcgt ttgtcgcctt tacgctgact ggcgctggca
     3961 ccgctgaggt tatcgaagct aatggctccc tttaatgctc tggagagcgg ggatgcctgt
     4021 acggtcaggc cactaccatt cagctgaact ttcgccccgc cttctgccca gaacacgcta
     4081 ttgctggtca gaaggttgcg atactccggc ttaatatgca gatcgatatc aaacgcgtta
     4141 gctcgcggac ggacggtaat aacttcacca acttcaaatt tacggtagag cactaccgat
     4201 cctgcctgca catccggcag cgtctctgca ctcaaactca cggttgtggt gggtaaatcg
     4261 ctaaggctgt tctccagcgc tttttccaga ttggcataca gtggatagct ggctttcatc
     4321 tcgcctttat cgcccggcag aatacgtatc ccgccgttaa tccattctga ggcgctggca
     4381 ccgagaaact caacgccatc cagccccacc ttcacgtcga cacggctgtt gacgacaaat
     4441 ttgctatcgc cttttaccag ttctcgatgc tgaggctcga tggcgacggt aaaggtgacg
     4501 cctttgctgg tgagtttacg atcgataacc aggcctacct gcacgccgtg aagaatgagc
     4561 ggctgacccg catcaatacc gtaactttcc ggcgcggtca gggtcagcgt cagaacatca
     4621 ggttcatgca gcagtgcttt ttcgcctggc acaacaacga actctttgcg tggctcgcca
     4681 tcgccgggta ccaactcgaa ggttttgcca gtcagcaggg cgctgagatt ggcatcgcta
     4741 agggataatt tcggattacg taattcgatg cgggtatttt cacgaagcag ggtaacgacg
     4801 ctgggatcaa ctgtcatttc cccagtgact ttaccaccag gatttaaatc cagtttagtt
     4861 agctgtccga cttccagtcc ctgatacatt aatggcgtcg agtcggcggt taatcctgcc
     4921 ccactcggca gttccagttt tattattacg ccacgctggc tgtgggccag atcttcatac
     4981 agaccaaagg tatcttccgc ctcggcaggt ttcgactctt caggtgaatc gaaggcaatc
     5041 gcaccgttaa ccagtgccgc cagactttcc agtttcacct tcgcgccact gatactgaca
     5101 ttggcatcaa cgccggaaac gttccagaaa cggctacctt ttttcaccag atcggtaaaa
     5161 cgccgctcga tcaggacatc aatcaccacg ccttgcttgt tgggattgat ggcatagtcg
     5221 tagacttttc ccacagggat cttgcggaaa tagaccaatg aaccgctgtt cagcgaaccg
     5281 agatcggggg cttgcaggtg gatcatcaga tcgccattgt ccagtcgata tttcggttgg
     5341 gtatcgagtg cgacaaagtg atcctgctct ttacctttac ctggcatcat gccgatatag
     5401 ttaccaccga cgagggcgtc cagcccggag acacctgcca acgatgcttt tggcgtcacc
     5461 agccagaact gagtctcttc gcgcagcgca tctttcatat cggacttgat gctgaccttg
     5521 acttcaatct tacgaagatc gtcgctgagg ctgatatcct gcactgttcc gacttcaacg
     5581 ccctgataac gaacaggcgt acggcccgga acaataccat ccgccgacat aaagtcgatg
     5641 gtgacggtat taccccggtc ctgataactg tcccaaatca gccaaccggc aatcattagc
     5701 gcgatgaaag gcagcagcca gaaaggtgag atacggcgtt tatttttaat ctgcgcttca
     5761 gtcgtcgaag cgggcgtttc ctgactcatg tgcatcccaa agtaagcggc tgtccagcca
     5821 ttccacagca agaatagtca aaattaccgc tgcgccgaaa taaaacgcag ccggtcccat
     5881 agtaaaagcg aggatctgat cgcgattaat cagcgacatg gttaaagata tgacaaacag
     5941 gtcgagcatc gaccagcgac caatccaggt caccatccgc agtaacagaa tgcgtgtgcg
     6001 taaaccttgc tggcatttaa aatgaatgct caacagtaaa gtgaacatga cgatcacttt
     6061 agtaaacggt accagaatac tggcgataaa cacgattccc gcaaccgcaa tgttgctact
     6121 tgccagcgac ataattccgg aaagaattgt atcttcctgc cgtcctccgt tcagataaat
     6181 gatagaaata ggcaacaggt tggcaggtaa caacaaaacg attgacgcta acagcgccgc
     6241 ccagcatttt tgcaaactat gacggcgacg caggcgtagc gggatatggc aacgcgggca
     6301 gcgaccacgc tgatcaggat agccggtaaa atggcaccca agacagacac gaagtttctc
     6361 gtccctacgc gtagcggggc gctgcggata aaatcgctcc cacagctcct cgacattaag
     6421 atgtgacaac gtcaccgtcg ttaaaatcac caacgccaca aaagagaaca agccgacacc
     6481 cgcctggata tgggcataat cctgtacctt tatagaagca acgccaatgc cgaccaggta
     6541 gatatccagc attacccact ctttaagtcg ctcaagcatc agcagtaccg ggcgtagatt
     6601 cattcccagt cggttaccaa accataaata agctatggag gtcaccagaa tgaggggggc
     6661 accgataacg cagaaaaaga ccatcgaccc cgttatcgca tcgccctgtt tggtcatttg
     6721 ccagatgcct tgcataacgt tggcgtcaat acgaatgcct aacagccaga tatgcaacag
     6781 cggttcgccc caggcaaacg gcatcagcaa cagcatggtg aaagccattg ccgccaggcg
     6841 cgttagcgac cagtcgcgcc cgtcgcgaat ttttgcctga cagcgcggac aataggcact
     6901 ctgatgagaa tttatctcgg gcaggctaaa cagcatgtca cattgcgggc aacgttggta
     6961 atcaccacgc ggcagttcct cgccgattgc cctcactgtt atcttttttg tcggcgtaat
     7021 ttgtggtgtg ttaagagcca tgacgtgcca aatgtaataa gaattaacat taatattaac
     7081 ccatgattgg aatgatcttg agcattaacg catttaatgc ttattttaat aggctggcag
     7141 ttccaggtaa gacaaccaag tgattatata atgaacaaaa cagaatttta cgcggattta
     7201 aatcgcgact ttaacgcgct gatggcggga gaaaccagtt ttctggcaac gcttgcgaac
     7261 accagtgcgt tgttatacga gcgtctcact gacgtaaact gggcaggttt ttatttgctt
     7321 gaggacgata cactggtact cggaccattt cagggcaaaa ttgcctgtgt ccggataccc
     7381 gtcgggcgcg gggtgtgcgg cactgcggtt gcccgcaatc aagtgcagcg tatcgaggat
     7441 gttcacgcgt ttgacgggca tattgcctgt gatgcggcga gtaattctga aattgttctg
     7501 ccgctggtgg tgaaaaatca gattattggt gttcttgaca tcgatagtac cgtcttcggt
     7561 cgctttacag acgaggacga gcaaggctta cgtcagcttg tggcacagct tgaaaaagtg
     7621 cttgcaacga cggattacaa aaaattcttt gcgagcgtcg caggataatc aacggataac
     7681 gtagcattta ctggtagcgt cattataatg acgcctgttc atgcctgcgc ttgttggcta
     7741 cgtccgttgt aatcaggaaa tttcatggaa aatcaaccta agttgaatag cagtaaagaa
     7801 gtaatcgcgt ttctggccga acgttttccc cactgtttca gtgcggaagg tgaagcgcgt
     7861 ccgctgaaaa tcggtatttt tcaggatttg gtcgatcgtg ttgccgggga aatgaacctg
     7921 agcaaaacgc aattacgatc cgctttacgt ctctacactt cgagctggcg ttatctttac
     7981 ggtgttaaac ccggcgcaac gcgtgtcgat cttgacggca acccatgcgg tgagctggac
     8041 gagcaacatg tagagcatgc tcgcaagcag cttgaagaag cgaaagcgcg tgttcaggca
     8101 cagcgtgctg aacagcaagc gaaaaaacgc gaagctgccg cagctgctgg tgagaaagaa
     8161 gacgcaccgc gccgcgaacg caagccacgt ccgaccacgc cacgccgcaa agaaggcgct
     8221 gaacgtaaac ctcgtgcgca aaagccggta gagaaagcgc caaaaacagt aaaagcacct
     8281 cgcgaagaac agcacacccc ggtttctgac atttcagctc tgactgtcgg acaagctctg
     8341 aaggtgaaag cgggtcaaaa cgcgatggat gccaccgtat tagaaatcac caaagacggc
     8401 gtccgcgtcc agctgaattc gggtatgtct ttgattgtgc gcgcagaaca cctggtgttc
     8461 tgaaacggag gccgggccag gcatgaacat gttttttag
//
LOCUS       DAMZET010000173         8485 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.173, whole genome shotgun sequence.
ACCESSION   DAMZET010000173 DAMZET010000000
VERSION     DAMZET010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.173"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..845
                     /gene="yeaX"
                     /locus_tag="POB34_003976"
     CDS             <1..845
                     /gene="yeaX"
                     /locus_tag="POB34_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HDI5920836.1"
                     /translation="HIIVQMSDGDNHYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSR
                     FLHQQVKVGDRLTISTPNNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQL
                     HYCSRNPESCAFRDELVQHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEA
                     LNEAVRSEAARLDIVADTLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIE
                     NNKAAKVECLCREGVCGTCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRL
                     VLDL"
     gene            complement(899..2014)
                     /gene="rnd"
                     /locus_tag="POB34_003977"
     CDS             complement(899..2014)
                     /gene="rnd"
                     /locus_tag="POB34_003977"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HDI5920837.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKVFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(2096..3781)
                     /gene="fadD"
                     /locus_tag="POB34_003978"
     CDS             complement(2096..3781)
                     /gene="fadD"
                     /locus_tag="POB34_003978"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HDI5920838.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(3986..4567)
                     /gene="yeaY"
                     /locus_tag="POB34_003979"
     CDS             complement(3986..4567)
                     /gene="yeaY"
                     /locus_tag="POB34_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HDI5920839.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(4607..5302)
                     /gene="tsaB"
                     /locus_tag="POB34_003980"
     CDS             complement(4607..5302)
                     /gene="tsaB"
                     /locus_tag="POB34_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDI5920840.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(5360..7270)
                     /locus_tag="POB34_003981"
     CDS             complement(5360..7270)
                     /locus_tag="POB34_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDI5920841.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            7402..7746
                     /locus_tag="POB34_003982"
     CDS             7402..7746
                     /locus_tag="POB34_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDI5920842.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            8109..8468
                     /locus_tag="POB34_003983"
     CDS             8109..8468
                     /locus_tag="POB34_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HDI5920843.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAIKMETGERSVIK
                     NPEYFSTYMQEELKALV"
BASE COUNT         2077 a         2270 c         2092 g         2046 t
ORIGIN      
        1 gtcacatcat tgtgcagatg agcgatggtg ataaccatta cagcaatgcg tattcactac
       61 tgagttcgcc gcatgacacc tcttgttatc agattgccgt tcggctggag gaaaactcgc
      121 gcggcggttc ccgctttttg catcagcagg taaaagtggg cgatcggtta acgatttcaa
      181 cgcctaataa cctgtttgcg ctaattccct cagccagaaa gcatctgttt atcgcgggcg
      241 gtattggtat cacccctttc ctgtcgcaca tggcagagct gcaacacagc gacgtcgact
      301 ggcagctaca ttactgctcg cgaaatccag aaagttgcgc atttcgtgat gagctagtcc
      361 agcatccgca ggctgagaaa gttcacttgc atcactcatc aaccggaaca cgactggaat
      421 tagcgcgatt attggcggat atcgaacctg gcacacacgt ttatacctgt ggtcccgagg
      481 cgctaaatga agcggtaaga agtgaagctg cgcgtctgga catcgtcgcc gatacgctgc
      541 actttgagca atttgctatc gaagacaaaa ccggcgatgc ctttaccctg gtgcttgccc
      601 gttccggaaa agagtttgtg gtgccggaag agatgactat tttgcaggtt attgaaaata
      661 ataaagccgc gaaagtggaa tgtttatgtc gtgaaggggt atgcggaacc tgcgaaacag
      721 caatactgga aggtgaagct gaccatcggg atcagtattt tagcgatgaa gagcgtgcca
      781 gccagcaaag tatgttgatc tgttgttcgc gtgcgaaggg taaacgcctg gtgttggatt
      841 tgtagtttgc caaagccgga tgtggcgctg agcgcgccca gtccggcttc ggaagatttt
      901 actgcggata ttcctgcaat aaattgtgta atgcttccgc catcagctca ccacgccagc
      961 cggaaatcag ctccggcaaa ttgttctgcg gtttcagttt ccagtgccag ttcagcagtt
     1021 ggttgatttg ccgacgcgat gccagcaatt cggcgctgat cttatgcgtt tcgctcacgt
     1081 cagtaatcag cgacttaatc gctttaaaca ctttacgata accaggcatg tccatcaggt
     1141 taagcatcgg ctgcggtaag gcctcttccg gcaatgtctg cgctttttcc accagcgcca
     1201 gcagcgtttt accgtgaaag cggatttcgc taccggataa acccaggctg tccagttcgc
     1261 ctaaacttcc cggcatataa cgcgcgaccg accacaaatg ctcttcacgc acgacaaagt
     1321 tcaccgccag atcacgctct cgcgccttgc gcagtcgcca gtcggctaac agttgcagac
     1381 aggccagttg gcgtgtgcgt aactgccagg cattggtgat atcacgccag gcatcttccg
     1441 gcgcaacgac ttcctgacga cgcatttgca tcaggcggca ttcatccagc gccgcaggta
     1501 gccagccgga ggcctccgtt tctaccataa gcttggcggt gatcggtaac agataccaga
     1561 catccgccgc tgcgtattca cactgacgtt cggtcagcgg tctggccagc cagtcggtgc
     1621 gcgattcact cttgtccagc gtaacgccgg aatactcttc caccatggaa gcgaaacccc
     1681 atgacatcgg gcgtccgcag aaggcagcaa ggatttgcgt gtcaatcaag ggttgtggta
     1741 attcgccaaa gacattgagg aacacttcca gatcttcact gcctgcatgg agaaattttg
     1801 tgatggacgg atcgcgcagg atcgctttca gcggtgacca gtcggtgatc ccgagaggat
     1861 caattagcgc cagatgctcg ccatcgaaaa gttgaatcaa ccccagctgc gggtaataag
     1921 tgcgcgtacg aacaaattca gtatccaggg ctatcgccgg aaaggcacgg acggcttcac
     1981 acaaagaagc cagcgcatcg tccgtggtaa tcatttggta attcaaattg ttttctcttt
     2041 agtgggcgtc aaaaaaaacg ccggattaac cggcgtctga cgactgactt aacactcagg
     2101 ctttattgtc cactttgccg cgcgcttcgt cacgtaattc tcgtcgcaaa atttttccga
     2161 cgttagattt cggtaactca tcacgaaact ccaccagctt cggtactttg tagcccgtga
     2221 gctgacggcg gcaaaaggtc accagtgact cttcggtaag cgatggatct tttttcacta
     2281 cgaagatttt caccgcttca ccactggagc cggaaggtac gccaacagcc gcgacttcct
     2341 gtacgccagg atgctgcatg acgacatctt caatctcgtt gggatagacg ttaaaaccgg
     2401 aaaccagaat catgtctttt ttacgatcga caatgcgcag gaacccttct tcatccatca
     2461 ccgcgatgtc gccggtgtgt aaccagccat ttttgatgat ctcatctgta gcatccggac
     2521 gctgccagta acccagcatc acctgcggtc ctttgacaca aagctcaccc ggttgacccg
     2581 gtggtacttc attatcatca tcatccacca gtttggcttc cgtcgacggc accggcaaac
     2641 cgatgctacc actatgataa tcaatatcat atgggttaac gctgaccagc ggcgcacact
     2701 cggtaaggcc atagccttcc agcagatact gtccggtcag tttcacccaa cgctctgcca
     2761 ccacttgctg cactggcatc cctccgcctg cggaaagatg cagactggag aaatccagct
     2821 gctggaactc tttattgttc agcaacgcat tgaacaaggt gttaacgccc gtgatagcgg
     2881 taaacggata tttcgctaac tcttttacca accctggaat atcgcgcggg ttagtgataa
     2941 gcaggttctg cccacccagt tcgataaaca gcaggcagtt aatggtcagg gcaaaaatgt
     3001 gatacagcgg cagcgccgtc accaccagct ctttgcccgg atgcaacagc ggaccatagg
     3061 tcgcgttaac ctgttccagg ttcgccagca tattgcggtg agtcagcatc gcgcctttcg
     3121 ccacaccagt ggtgccgccg gtgtattgca gaaaagctaa atcttccggc accagttcgg
     3181 gtttgacgta ctgcatccgg tagccgttat gcagtgcgct acgaaatgaa atggcatctg
     3241 gcagatggta tttcggcacc aaacgcttga tgtatttaac aacgaaattg actaccgtgc
     3301 cttttgccgt agatagctga tcgcccatac gggtcagaat tacgtgctga acggcggttt
     3361 tatcaaccac tttttccagt gtgtgagcaa agttagacac gataacaatc gccgatgcgc
     3421 cgctatcgtt aagctgatgc tcaagctcac gcggggtata caacgggtta acgtttacga
     3481 cgatcatccc ggcacgcaaa ataccaaaca gcgccaccgg atattgcaat aaattaggca
     3541 tcatcaacgc aacgcgatcg cctttcttca gccccaaccc ttgttgcaaa taagcggcaa
     3601 acgcgcgact gcgttcttcc agcttgcgga aggtcattac ctcccccata ttcacaaacg
     3661 caggttgatc ggcgtagcgc gcgaccgact gctcaaacat atctaccaga gattgataac
     3721 ggtcagggtt gatctccgtc ggaacgtccg cgggataacg gttaagccaa accttcttca
     3781 attcttcacc tctaaaatgc gtgttcgtcg tcatcgcaac cccaaatgat atacatgccg
     3841 ttaacataat attaactcat cataccagct tgataattac ccaacgaaaa ggttgcgaag
     3901 cgcgtcacta tttattttta tctttaccgt aagaatgcag aaacagcgga ccagccgctg
     3961 tttctttttc tttaaaaaca agcaattatt cagttacaac tgtttgtact ctcgcggggc
     4021 cgggattata ccagccccat ccgccgtagc catagggcca gccacgaccg ccataaaacc
     4081 atggatcaat cggctgaggc ggcataatca cctgctgggt taaatgccaa cgtttgtaac
     4141 ccgttacttg catcaccata aatttatagg gcgtattgcc gattttgccg tcaaccgcac
     4201 cagtgattgg cccgactacc gtaacaagtt gtccacggaa atccaccggg tccagaaaac
     4261 cgttcacatc ggcataaatg cgaccgcgag aaggttctcc cagcgtcggt ctggctccgc
     4321 tgtccagcgg tacggtagca atttccaggc gggttttccc ttgctggttt tgtaccgcaa
     4381 ccactttgcc accaaagcgt gcctcctgac caacgtacag ctgcggcgca ctcatcaccc
     4441 gaactaaatc ttgttgcggc gtgggactgc tgcctttaat ggcgtccggc acagtgacac
     4501 aaccgctcag cattagcgca aacgtacctg ccagtatgcc tttgataaca ttcttttgaa
     4561 ccgccatgat gcgactcctt tttttctcag ggcatactct taagattcat tctttgcccg
     4621 gaagtttctt ccatgcgacg ttgttacgta aataaaccgg ttcggcatgt tccaccgcca
     4681 ccgttttacc ctcggcaaac atctgacacg caatcggcag catatcttcc gccgctggca
     4741 gtaaaacttc tccgtcgcgc aaaaccagcc cgctctcttt accgagatcc ggccaggctt
     4801 gccagcccgt ccctaccgtt acccattcgc cggaaagctg ttgcattcgt tcatgaacga
     4861 gttcgggttt gagtacggct tcggtttctt caccgcgcca gataccgttt tcatcacgct
     4921 gatattcggc ccagtaaact tcgcccattc gcgcgtcaat ggctgccagc acgcgggttg
     4981 cgccgttttt gcgccacgcc ccttgcgcca tcgtcattag cgtggagacg ccaatcatcg
     5041 gtagctctgc gccaagtgcc agcccttgcg caatgccaat accaatgcgc acgccggtaa
     5101 agctaccggg gccgcgcccg taagccagag cgttaatatc agtcagggaa gttccgctgg
     5161 tggtcaggat atcctgcacc atcggtaaga ttcgttgagt atgttcacga gggcaaagct
     5221 caaaatgagc gttgacagta ccgtcgttcc acagggcgac agagcaggcc tctgtcgcgg
     5281 tatcgatagc cagaattcgc atgggtcttc gtgcttagat caataaaaag gcgcgcatca
     5341 taccatactc cgtaacaaat tacctggagg atggtatcgc aaggaaacga accgcacggg
     5401 caatgtcacg agtgcgtggc gcgggcggca gactggcgag aaacgtcgcg ccgtaaggac
     5461 gcatcaccag ccgattgtca caaatcacca gcacgccacg atcgtcggcg tcgcgaatca
     5521 gtcgccctac cccctgtttg agagtaatga cggcatctgg tagttgcact tcatcgaacg
     5581 ggtcgccacc gcgcaaacga caatcttcca tgcgcgcttt taacagtgga tcatccggcg
     5641 aggtaaacgg caacttgtcg ataatcacca atgacaatgt atcgccacgc acgtccaccc
     5701 cttcccagaa actactggtt gccacaagaa gcgcgttacc ggcgctgaca aattgctgca
     5761 acagttgccc tttgctggtt tccccctgca acaaaacggg aagcgtcatg gtagcgcgga
     5821 actgctcggc cagatcacgc atcatggcgt gcgaggtaca aagcataaaa caacgaccgt
     5881 tgttagcttc gatgatcggt cgcagcattg ccgctaactg gcgagcagaa cctggctggt
     5941 tggtttgcgg cagattgcgc ggcacacaga gtaacgcctg gcggctgtaa tcaaacgggc
     6001 ttggcaatag caacgactcc gcctgttcta tcccaagccg cgaggtgaaa tgatgcagat
     6061 cgtcgttcac cgacagcgtt gctgaggtga agatccagct accaggcttt tgcgccatta
     6121 actctttgaa tttatccgcc acgctgagcg gcgtgagagc cagagtaaaa tggcgcgaag
     6181 tgcattcgta ccagtagctg tagcccggct gattgatctc ttttagccgc ttcagccgtg
     6241 tgcgatacaa cgtagcgcgc tcaaatgccg catccagcaa ggcggaacgc cccagtgaca
     6301 gtttcgccac gtcataacaa agttccaggg tgtcatcgag cagtaaaaat gcccgctgaa
     6361 tttgcggatt agctaacagc tcacgcaggt taccgcgata acctggctca ccgagttgca
     6421 gacgaaaatc ctgcgcactc tgggcaagac gatcagcgca cttttgtaac tgctgggtgt
     6481 cttttaattc ggtgcggtaa gcgatggtga tgtcttttgc caggtcgagc agttgtcgac
     6541 tggagagtga ctgaccaaaa tactggctgg caatgtccgg tagctggtgg gcttcgtcga
     6601 agatcatgac gtccgcttcc gggatcagtt cgccaaatcc actctcttta accaccatat
     6661 ccgccagaaa gagatgatgg tttaccacca ccacatcggc gtccatcgct tttttacgtg
     6721 ctttgaccac aaagcaatct ttatacatcg ggcagtcgct gccaagacag ttgtcgttgg
     6781 tgctggtgac cagcggccac gcctgtgaat cttccgccac gctgacgcag gtgctgatat
     6841 caccatcgac tgtttgatta gaccaggagc gcagcaggat cacatcgctt aagatttgta
     6901 ccggcagatc gccccccgcc agcgcctgct gttcgagacg ctcgaggcag aggtagtttg
     6961 agcgcccttt cagcagcgcc acgttgcccg tatatttcaa tgcctttgag actgttggca
     7021 aatcgcggct gtagagctga tcctgcaacg cttttgagcc ggtcgagata atgactttct
     7081 ttttcgcccg cagcgcagga gccaggtaag cgtaggtttt gcccgtaccg gttcctgctt
     7141 ccaccaccag cggctggcct ttttctatcg cctgggtgac ggctaccgcc atctgtcgct
     7201 gtggttctcg cggcttaaag cctggtatcg ctttcgccag ctgaccgtct ggtgcaaaat
     7261 cgtccgtcac actaccccct gttgatttga acagggatta tgtcaggatg agggcgcttt
     7321 cgccagttga agtggtgacg gcgacctcac attgtggcag tctttgcagc acgaaaatgg
     7381 aaggataatg aggacaaaat gatgactatc gttcgtatcg atgctgaagc ccgctggtct
     7441 gatgtggtaa tccacaacaa cacgctctac tacactggtg taccggaaaa cctcgacgcc
     7501 gatgcctttg agcaaaccgc caacacgctg gcgcagattg atgccgtgct ggaaaaacag
     7561 ggcagcaata aatcgagcat tctggatgcc accattttcc tggccgataa aaacgacttc
     7621 gcggcgatga ataaagcgtg ggatgcctgg gttgtcgcgg gtcatgcgcc ggtgcgctgc
     7681 acggtacaag cgggtttgat gaacccgaag tataaagttg aaattaagat tgtggctgcg
     7741 gtgtaagctt tatcgaagca aaataagtca gacgataatt tatcgataat actggtcggt
     7801 tttacataaa tcgaccagag attaagatat tacctgttga tgctcctgct aattcattat
     7861 ttatttttat aatttcaatt ttatctataa aactaactat ataaataacc ataaccatgc
     7921 cgttgtgttc gccctctgac tagcaactct ggttttcttt cttagctgaa taatctggtt
     7981 atagcgtgag gactgaccgt taaatttctc catcttactg gataacacac catgagctgc
     8041 tacctttaaa atacctgaat gtcaacaggt taactcgtgc catatcgtta taatcccttt
     8101 gatattagat gcaaattaag gtcatatata gtcttatcga taacatggtt aattttaagg
     8161 ataagaatat gcctgctgta atagataaag ccctggattt cattggtgcc atggatgtat
     8221 cagcgccaac accaagttcg atgaatgaaa gcacggcgaa gggaatcttt aaatatttaa
     8281 aagaactggg agtacccgcg agtgccgctg atattacggc gcgagctgac caggaaggct
     8341 ggaaccctgg gttcacggaa aaaatggttg gatgggcaat aaaaatggag acaggtgaac
     8401 gctctgtgat taaaaatcct gaatactttt caacatatat gcaggaagaa ctaaaagcac
     8461 tggtctgagt taaatttata tcagc
//
LOCUS       DAMZET010000174         8470 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.174, whole genome shotgun sequence.
ACCESSION   DAMZET010000174 DAMZET010000000
VERSION     DAMZET010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.174"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            112..1392
                     /gene="wcaK"
                     /locus_tag="POB34_003984"
     CDS             112..1392
                     /gene="wcaK"
                     /locus_tag="POB34_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HDI5920844.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGS
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISYPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNTRLNEAVSRERQTGMQMVQSVLERIGEAK"
     gene            1389..2609
                     /gene="wcaL"
                     /locus_tag="POB34_003985"
     CDS             1389..2609
                     /gene="wcaL"
                     /locus_tag="POB34_003985"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HDI5920845.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWAKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPLHADVFIAHFGPAGVTAAKLRELGVICGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISNLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVIEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVIKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            2620..4014
                     /gene="wcaM"
                     /locus_tag="POB34_003986"
     CDS             2620..4014
                     /gene="wcaM"
                     /locus_tag="POB34_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HDI5920846.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLNV
                     SHANYAILRQGFHNQIIGTNITNCKFSDLQGDAIEWNVAINDSDILISDHLIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKRETLLSLTNIHAVNEKGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            4189..5082
                     /gene="galF"
                     /locus_tag="POB34_003987"
     CDS             4189..5082
                     /gene="galF"
                     /locus_tag="POB34_003987"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HDI5920847.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKHMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLNE"
     gene            5395..6537
                     /locus_tag="POB34_003988"
     CDS             5395..6537
                     /locus_tag="POB34_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDI5920848.1"
                     /translation="MKKILLISMSLGKGDYGGGIVSNTNFFALKELEHYELFSVGIVK
                     NTNDAPNFINMVLPGNASKFSTAINNILGFAGQLNNKTTKNIKYIIDEFEPDIVYLDS
                     SLLGCIASYCKKKHKTIQIITFFHNIEFDFEIARIMSGLLHFFPSLISTTLAEYAAVR
                     YSDKIIALHKKDSFRLEEKYGRKADYIVPVCIKDTQREKSFKLVNEKKKEGKKLKVGF
                     IGTAFFANVESAKIISQYIAPKVEGIANFYICGNGFEKYKALNSTNVNVSGYIDSLDD
                     FYNEMDVMIFPIFSGAGMKVKIAESLMYNKPILASAFALVGYEKIIDGTNVISCESHE
                     SFVYHIKQFRRDNNTLYNRKSYYKYFSDKACLHYFRNILREIVNNE"
     gene            6558..7667
                     /locus_tag="POB34_003989"
     CDS             6558..7667
                     /locus_tag="POB34_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDI5920849.1"
                     /translation="MNIIQLSKFYPPIFGGIEQVAQDITEGMQNLCNVDVLSVNNSSK
                     TIYCKNIIRASLLFTLLSTPVSISYILIWSRIRNNYDIIHVHLPNPLAIIALLLFPPK
                     APVVVHWHSDIVKQKIALKFFRPLQNLFLNKVEKIIVTSEIYGSSSPQLQRFQDKIIC
                     IPIGIKSERLPKNETLLKHLKEKYKNKKIVFSLGRLVYYKGFENLVNAANFLPEDTII
                     LIGGCGELYDELADSILSNKLEGKVVLLGEIKYEQLSAYYQVCDVFCLPSIHESEAFG
                     VVQIEAMSYGKPVVSTNIKNSGVPWVNENGISGVVVEPNEPHELAKAILTILNNPAGF
                     SLGALERYRKLFTRDKMISSLIGLYQNIKIGNEKE"
     gene            7664..>8470
                     /locus_tag="POB34_003990"
     CDS             7664..>8470
                     /locus_tag="POB34_003990"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HDI5920850.1"
                     /translation="MILPVILAGGAGSRLWPLSRLLYPKQFLCLKGELSMLQTTICRL
                     NGLGCENPVVICNEQHRFIVAEQLYQLNKLTENIILEPAGRNTAPAIALAALATQWHK
                     PDSDPLLLVLAADHVIADEDAFRAAVSNAMPYAEAGKLVTFGIVPRHPETGYGYIRRG
                     AVSAGEQEGVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELKKYRP
                     DILDACKKAMSAVEPDLNFIRVDEEAFLACPEESVDYAVMERTADAVVVPM"
BASE COUNT         2415 a         1815 c         2025 g         2215 t
ORIGIN      
        1 gtgctcgggt cttgtaggcc tgataagacg cggccagcgt cgcatcaggc aaaaaacggc
       61 aatgaacaca acaaacacag cctcacctgg catcaaattt atgaggaaaa aatgaaatta
      121 cttattctgg gcaaccacac ttgcggcaat cgcggcgaca gcgccatctt gcgcggctta
      181 cttgatgcca tcaacatcct caatccacat gccgaagtgg acgtcatgag ccgctatccg
      241 gtcagttctt cctggctgct caaccgcccg gtaatgggcg atccgctgtt cctgcaaatg
      301 aaacaacaca acagcgcggc gggtgttgtc gggcgcgtta aaaaagtcct ccgtcgccgc
      361 tatcaacacc aggtattgct ctcgcgcgtc accgacactg gcaagctgcg caatattgcc
      421 atcgcccagg gattcactga cttcgtgcgc ctgctgtcag gttacgatgc cattatccag
      481 gtcggcggat cgttttttgt cgatctctac ggcgtgccgc agtttgaaca tgcactttgc
      541 acatttatgg cgaaaaagcc gctgtttatg attggtcaca gcgtcggttc cttccaggat
      601 gagcaattta accaactggc gaactatgtc tttggtcatt gcgacgcgct gatcttgcgc
      661 gaatcggtca gccttgattt gatgaaacgc agcaatatca ctaccgcaaa agtggaacat
      721 ggcgtcgata ccgcgtggtt ggtcgatcac cacaccgaag acttcactgc cagctatgcc
      781 gttcaacact ggctggacgt tgccgcacaa cagaaaacgg tggcaattac cctgcgcgaa
      841 ctggcaccgt tcgacaaacg tctcggcacc actcagcaag cgtatgaaaa agcctttgcc
      901 ggggtggtca atcgcattct cgacgaaggc tatcaggtca ttgcgctctc cacctgtacg
      961 ggcatcgaca gctataacaa agacgaccgc atggtggcgc tcaacctgcg ccagcatatc
     1021 agctatcctg cccgttacca cgtagtgatg gatgaactca atgatctgga aatgggcaaa
     1081 attctcggtg cctgtgaact caccgtcggt acgcgcctgc actccgccat tatctcaatg
     1141 aactttgcta ctccggcgat tgccatcaac tatgaacata aatccgccgg gattatgcaa
     1201 caactggggc taccagagat ggcaattgat atccgtcatt tactggacgg cagcttgcaa
     1261 gcgatggttg cggatacctt aggccagctt ccggcgctga acacacgact taacgaagcc
     1321 gtcagtcgtg agcgtcagac gggaatgcag atggtgcagt ccgtactgga acgcatcggg
     1381 gaggcgaaat gaaggtcggc ttcttcttat tgaaatttcc gctgtcgtcg gaaaccttcg
     1441 ttcttaacca aattaccgcg tttattgata tgggctttga ggtggagatt gtcgcgctgc
     1501 aaaaaggcga tacagagaac acccacgcgg catgggcgaa atacaacctt gccgccagaa
     1561 cccgctggtt acaggacgaa cctacgggca aagtggcgaa actgcgccac cgcgccagcc
     1621 agaccttacg cggcattcat cgtaaaaata cctggcaggc gctcaacctc aaacgctatg
     1681 gtgccgagtc gcggaacctg attttgtctg ccatttgtgg tcaggtcgca acaccgttac
     1741 atgccgatgt ctttatcgct cattttggtc cagcgggggt aaccgcagca aaactccgcg
     1801 aactgggtgt catttgcggc aaaattgcta ctatcttcca cggtattgat atctccagtc
     1861 gggaagtgct caaccactac actcccgaat atcaacaact gtttcgccgt ggcgacctga
     1921 tgttaccaat aagcaatctg tgggccggaa ggctgcaaaa aatgggctgc ccgagggaaa
     1981 aaatcgccgt atcgcgcatg ggcgtggaca tgacgcgttt tagcccgcgt ccggtgaaag
     2041 cgcccgcaac gccgctggaa atcatctccg tcgcacgctt aaccgagaaa aaaggcctgc
     2101 atgtggcgat tgaagcctgt cggcagttga aagagcaggg cgtggcattt cgctatcgca
     2161 tcctcggcat tggcccgtgg gaacgacgcc tgcgtaccct catcgaacaa tatcaactgg
     2221 aagatgtgat agagatgccg ggctttaaac cgagccacga agtgaaagcg atgctcgacg
     2281 acgcggatgt cttcctgttg ccatcggtta caggtgcgga tggcgatatg gaaggcattc
     2341 cggtggcgct gatggaggcg atggcggtcg gcattccggt ggtttctact ctgcatagcg
     2401 gaataccgga actggtggag gctgacaaat ccggctggct ggtgcctgag aacgatgctc
     2461 gcgcactggc gcaacgactg gcggcgttta gccaactgga taccgacgaa ctggctccgg
     2521 ttatcaaacg tgctcgcgaa aaagtcgaac acgattttaa ccagcaggtg attaatcgag
     2581 aactcgccag cttgctgcag gctttataga ggagaacgca tgccatttaa aaaactctcc
     2641 cgacgcacct tcctgacggc aagctcggcg cttgccttcc tccatacccc tttcgcccgt
     2701 gcacttcctg cccggcaaag cgttaacatt aacgactaca accctcacga ctggatcgcc
     2761 tcatttaaac aagctttcag cgaaggacaa acggtcgtcg tgcctgccgg attggtttgc
     2821 gacaatatca acaccggcat cttcattcct cctggcaaaa cgttacacat ccttggaagc
     2881 ctgcgcggca acggcagagg gcgatttgtc ttacaggacg gcagccaggt gacaggggag
     2941 gagggcggca gtatgcataa catcaccctg gatgtgcgtg gttctgactg caccatcaaa
     3001 gggctggcga tgagcggctt tggtccggta acgcagattt atatcggcgg caaaaacaaa
     3061 cgggtcatgc gcaacctgac catcgataac ctgaacgtta gccacgctaa ttacgccatc
     3121 ttacgccagg gatttcataa tcagattatc ggtaccaaca tcaccaactg taagttcagc
     3181 gacttacagg gcgacgccat tgaatggaat gtggcgatta acgacagtga tattttgata
     3241 tctgaccatc tcatcgagcg catcaactgt accaacggca aaatcaactg gggaatcggc
     3301 ataggccttg cggggagtac ttatgataat aactacccgg aagaccaggc agtgaaaaac
     3361 tttgtcgtgg cgaatatcac gggatcggat tgtcggcagt tgatccatgt tgaaaatggt
     3421 aaacattttg ttattcgtaa tatcaaagcc cgcaatatca cgccggattt cagtaagaaa
     3481 gcaggcattg ataatgcaac agtcgctatt tacggttgtg acaatttcgt gattgataat
     3541 attgaaatga ttaatagcgc cgggatgtta atcggctatg gggtaattaa aggcaaatat
     3601 ctctcgatac cgcaaaattt cagagtaaat aatattcaac tggataacac ccatcttgct
     3661 tataaattgc gcggcatcca aatctccgcc gggaatgctg tctcctttgt ggcgctgact
     3721 aacattgaga tgaagcgtgc gtcgctggag ttacacaaca aaccacaaca cctttttatg
     3781 cgtaatatca atgtgatgca ggaatcctct gttggaccag cattgagcat gaacttcgac
     3841 atgcgcaaag acgttcgcgg cgtctttatg gcaaagcgtg aaacgctgct ttcactcacg
     3901 aatattcatg ccgtgaatga aaaagggcaa agctccgtcg atatcgacag gattaatcac
     3961 catattgtta atgtggaaaa gattaacttt agattgccgg aacggaggga gtagatttgc
     4021 gaccattcct ggaaaaatgg agccatactt aggaacaatg ctactgcaat ccacaacgaa
     4081 gcggcgtaac atcacaagta attcagtaat caattcaggg taattgatgc tggcgaaaaa
     4141 aatcgaacaa gctataattc agcaaccatt ttacaggtgg atgaaataat gacgaattta
     4201 aaagcagtta ttcctgtagc gggtcttggg atgcatatgt tgcctgccac taaggcgatt
     4261 cccaaagaga tgctaccgat cgtcgacaag ccaatgattc agtacattgt tgacgagatt
     4321 gtggctgcag ggatcaaaga aatcctcctg gtaactcacg catccaagaa cgcggtcgaa
     4381 aaccacttcg acacctctta tgaattagaa tctctccttg aacagcgcgt gaagcgtcaa
     4441 ctgctggcgg aagtgcaatc tatctgcccg ccgggcgtga ccattatgaa cgtgcgtcag
     4501 ggcgaacctt taggtttagg tcattccatt ttgtgtgcac gacccgccat tggtgacaat
     4561 ccatttgtcg tggtgctgcc agacgttgtg atcgacgacg ccagtgccga cccgctgcgc
     4621 tacaaccttg ctgccatgat tgcgcgcttc aacgaaacgg gccgcagcca ggtgctggca
     4681 aaacatatgc cgggtgacct ctctgaatac tccgtcattc agactaaaga accactggat
     4741 cgtgaaggta aagttagccg cattgttgaa ttcatcgaaa aaccggatca gccacaaacg
     4801 ctggattcag acatcatggc cgtgggccgt tatgtgcttt ctgccgatat ttggccggaa
     4861 cttgaacgca ctcagccagg tgcatgggga cgtattcagc tgactgatgc cattgccgaa
     4921 ctggcgaaaa aacagtccgt tgatgccatg ctgatgaccg gcgacagcta cgactgcggt
     4981 aaaaaaatgg gttatatgca ggcgtttgtg aagtatggac tacgcaacct caaagaaggg
     5041 gcgaagttcc gtaaagggat tgagaagctg ttaaacgaat aatgaaagct tgatcggata
     5101 tgacggctgc gaaaaattgg aataattgca ggttattttg aatatggggg atttctaaaa
     5161 caccccatta acaatgtgtt gacaattgat tttgaccaag ttgttaatgt ttttcttgcg
     5221 ttttcaattg gttgattaga taatattata tagatctata atctattcta gttgggttgg
     5281 gttggggaaa ttgttcgatc ggaatatata gtttctggtg ttctgatagc cataagtcag
     5341 gggcggtagc gtggattttt attgcgtaaa aacgtcagta aacatagagg tttaatgaaa
     5401 aaaatactat taatatccat gagtttggga aagggcgatt atggtggtgg tatagtatca
     5461 aatacaaact tttttgcttt aaaggaatta gagcattatg agttattttc agtaggtatt
     5521 gtaaaaaaca ccaacgatgc accaaacttt attaacatgg ttttgcctgg caacgcgagt
     5581 aaattctcaa ctgcaattaa taatatatta gggtttgccg ggcagttaaa taataaaact
     5641 acgaaaaaca taaaatacat aattgacgag tttgagcctg atattgtata ccttgattca
     5701 agccttctgg ggtgtatagc ttcttattgc aagaaaaaac ataaaactat tcaaataata
     5761 acgttctttc ataatattga gtttgatttt gaaattgcac gtattatgtc agggttattg
     5821 catttttttc cctcgttaat atccacaact ctcgcagaat atgcggctgt aaggtatagt
     5881 gacaagatta tcgcattaca taaaaaagat tcttttagac tagaggaaaa atatggaagg
     5941 aaggctgatt atatcgtacc cgtttgcatt aaagatacac aaagagaaaa aagttttaaa
     6001 ctcgtcaacg aaaagaaaaa agaaggcaaa aagttaaaag ttggctttat aggtacagct
     6061 ttttttgcaa atgtagagtc tgctaagatt atatctcaat acattgcgcc aaaggtggag
     6121 gggattgcta atttttatat atgtggcaat ggatttgaaa aatataaagc attgaattca
     6181 actaatgtaa atgtttcagg ctatattgat tctttggatg atttctataa tgaaatggat
     6241 gtgatgatat ttcctatttt tagcggcgca ggaatgaagg tgaaaatagc agagtcatta
     6301 atgtataata aaccaattct agcttctgca tttgcattag ttggttacga aaaaataata
     6361 gatggaacta atgtgattag ctgtgagtct cacgaatctt ttgtttatca tataaaacaa
     6421 tttagaaggg ataataacac attatataat aggaagtctt attataaata tttctctgat
     6481 aaagcatgtc tgcattattt tagaaatata ttacgagaaa ttgttaataa cgaatagata
     6541 gatggactaa taacgtcatg aatataatac aattatcaaa attctaccct ccgatatttg
     6601 gtggaataga gcaagttgca caggacataa ccgaagggat gcaaaatttg tgcaatgttg
     6661 atgttttaag cgtaaataac agttctaaaa caatatattg taagaatata attcgagctt
     6721 cattactatt tacactatta tctaccccag tatcgattag ctatatacta atctggagta
     6781 gaatcagaaa taattatgat ataattcatg ttcatttgcc aaatcctctt gctattattg
     6841 cactgttgct gttcccgccc aaagctcccg tcgtagtgca ttggcatagc gatatagtaa
     6901 aacagaaaat agcgttaaag ttttttcggc ctttacagaa tttatttttg aataaagtag
     6961 aaaaaattat tgtgacatct gaaatttatg ggagttcttc tcctcaatta caaagatttc
     7021 aagataaaat aatttgtata ccgatcggta taaaatcaga gcgattacct aagaatgaaa
     7081 cgctattaaa gcatctaaaa gaaaaatata aaaataaaaa gatagttttc tctcttggga
     7141 ggctggttta ttacaaaggg tttgaaaatc tagttaatgc agctaacttt cttcctgaag
     7201 atacaataat ccttattggt gggtgcggtg agttatatga cgagttggct gatagcattt
     7261 tatccaataa actagaaggg aaagtggttt tgctggggga gataaaatat gaacagcttt
     7321 ctgcttatta tcaggtttgt gacgtatttt gtcttccttc cattcatgaa tcagaagctt
     7381 ttggcgtggt tcaaattgag gcaatgagct atggaaaacc tgttgtatcc acaaatatta
     7441 aaaatagtgg ggtcccatgg gtaaatgaaa atggtatttc aggtgttgta gttgaaccta
     7501 atgagcctca tgaactagcc aaagctatct tgacaatttt aaataatcca gctggttttt
     7561 ccttaggggc attagagcga tatcgcaaat tattcactag agataaaatg atctcaagtt
     7621 tgattggact ttatcagaat attaaaatag gaaatgaaaa agaatgatct tacctgttat
     7681 tctggccggt ggtgcaggta gtcgcctttg gccactttcc cgcttacttt accccaaaca
     7741 gtttttatgt ctgaaaggcg agctttctat gctgcaaaca actatctgcc gcctgaatgg
     7801 tttggggtgc gaaaatccgg tggtgatttg taatgagcag caccgcttta ttgttgcgga
     7861 acagttgtat caactgaaca aactcaccga gaacattatt cttgaaccgg caggacgtaa
     7921 tactgcacct gctattgcgc tggcggcgct agcaacacaa tggcataaac cagatagtga
     7981 tccgttactg ttggtgctgg cagcggatca tgtgattgct gatgaagacg cattccgcgc
     8041 cgctgtgagt aatgctatgc cgtatgccga agcgggcaag ttggtgactt ttggcattgt
     8101 gccaaggcat ccagaaacag gttatgggta tattcgtcgt ggtgcagtgt ctgcgggcga
     8161 gcaggaggga gtagcctttg aagtggcaca gtttgtcgaa aaaccgaatc tggaaaccgc
     8221 gcaggcctat gtggcaagcg gcgaatacta ctggaacagc ggtatgttcc tgttccgcgc
     8281 cggacgctat ctcgaagaac tgaaaaagta tcgtccggat attctcgacg cctgtaaaaa
     8341 agcgatgagc gccgttgagc cagatcttaa ttttattcgt gtagatgaag aagcgtttct
     8401 cgcctgtccg gaagagtcgg ttgattacgc ggtaatggaa cgaacagcgg atgctgttgt
     8461 agtaccgatg
//
LOCUS       DAMZET010000175         8355 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.175, whole genome shotgun sequence.
ACCESSION   DAMZET010000175 DAMZET010000000
VERSION     DAMZET010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.175"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..191)
                     /locus_tag="POB34_003991"
     CDS             complement(<1..191)
                     /locus_tag="POB34_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920851.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGG"
     gene            complement(184..2463)
                     /locus_tag="POB34_003992"
     CDS             complement(184..2463)
                     /locus_tag="POB34_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920852.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFFSHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQQNNGDVLVVCGGWHAPALAKMWRECPQDIN
                     TPELPSLADAITGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHNLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEEHWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLSGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DANFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(2474..3562)
                     /locus_tag="POB34_003993"
     CDS             complement(2474..3562)
                     /locus_tag="POB34_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDI5920853.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            complement(3869..4186)
                     /locus_tag="POB34_003994"
     CDS             complement(3869..4186)
                     /locus_tag="POB34_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920854.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
     gene            complement(4247..5686)
                     /locus_tag="POB34_003995"
                     /pseudo
     CDS             complement(4247..5686)
                     /locus_tag="POB34_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            5828..6967
                     /gene="cydB"
                     /locus_tag="POB34_003996"
     CDS             5828..6967
                     /gene="cydB"
                     /locus_tag="POB34_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDI5920855.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            6982..7095
                     /gene="cydX"
                     /locus_tag="POB34_003997"
     CDS             6982..7095
                     /gene="cydX"
                     /locus_tag="POB34_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HDI5920856.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            7095..7388
                     /gene="ybgE"
                     /locus_tag="POB34_003998"
     CDS             7095..7388
                     /gene="ybgE"
                     /locus_tag="POB34_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HDI5920857.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            7538..7942
                     /gene="ybgC"
                     /locus_tag="POB34_003999"
     CDS             7538..7942
                     /gene="ybgC"
                     /locus_tag="POB34_003999"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDI5920858.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            7939..>8355
                     /locus_tag="POB34_004000"
     CDS             7939..>8355
                     /locus_tag="POB34_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HDI5920859.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPY"
BASE COUNT         1903 a         2146 c         2210 g         2096 t
ORIGIN      
        1 ccacctaatc caccagagcg ttcaccacgc tggagccgtt caggatcgcg cccatacagc
       61 cactccagcg cgtgatctat ctgccgggcg ttgtcatcca gcccacaaag cgtcgtttct
      121 gccgcttcgc caagaattaa tcgccagcgt tgtagctcac gggtggtcag aagatcgttc
      181 agttcagaca tggaaaactc cccagtgttg cagcgatgcc agtgcctgct gttcgagttg
      241 ttgatgatgt gcaatggctt gcggtggaca atgcaacggc atttgcagtg ccgaaacggg
      301 aagttgcgcc agttgataat gctcaagcac ctgatgcgcc agcgttccgc gttctcgtgg
      361 cggtagccag gccatcgccg cgcgtaaatc gggcagggca ttgataaaat tggcatcact
      421 cagttgattc aaatggctgc tgaaaccggc gataaatgca ggttgacagg ccagttgatg
      481 acgggctaat gccagcaaac cgtgcagcgc ctcgccggat tgtgccgggg ataactgcgc
      541 cagcatcgtc agcgccgctt cggcactggc gttgggatgt tccagacgca ttagtgcgcc
      601 aagagccgcg ccgcgatcca gcgccggggc atgaatcgcc tgactgcgtc gctccagtaa
      661 ggctaccgcc gcagaaagcg aaacgccgga taaattaacg ccgctatgta gatcgcgcag
      721 aatatggcaa agcgcttgcc agctatgcag gtgagcgtga aactcctttt catccggtct
      781 gccgttagat tcacacagcc acagcgtgcg atcgatagcc gcgcataacg tcgtttgtaa
      841 tatctgcgcg ccttgcatac cgctaatttc atccagccgc cataaggcat atagcacttc
      901 cagcgcctgg cccatttcgg caaattgatt ttcctgggcg attaataatg tgagttgctc
      961 cagtaattgt tgactgaagg acgccagacc cgctaacgcc gcctggctaa gacatgtggt
     1021 gatactgccg attccacccg cgtccagcat atcggcttct aatttatggc gtgcggcttc
     1081 ctgaagtgtt gcaccaaagc atgcggcctc aatcaatgcc gcatgctggc taagcgggcg
     1141 ggttaatttc cagtgttctt caccgttgcc tgcaagtgtc agtgtacttc cctgctggcg
     1201 tacaatccca gggatctcca gtattgccag ccgatgtaac acctgacttt gcgccagccc
     1261 atcgggggta aagcgattta aggtaagctc cgccggaaga gagattgcgg tacgttccag
     1321 ttcgcaggtg acatcttttg gcagaggcgg ttgtggcgtg gaaggggcaa gtttaccgaa
     1381 tccgtcaccc gctaatgtgt ctatcaacgt tagcagaatc ggatcggtat cgggatgaat
     1441 aacgccgcga tagctccacg gcaacggtgc attcagggct tctttaatca acgagcctgc
     1501 tatggcatcc agccagtcag tgcgtaacgg tagtgtatga ccgcgcaact gtgccagtgc
     1561 cattgcatgc agatgtgcgg cagccatatc cgcggtcgaa gcaggcaagt tgtgctggcg
     1621 caaacgggtg agaaccgttt ttagcagttg ttcaccggcc tgctgtaagc cccactgcca
     1681 gcaccagttt tgccagaccg gggcaggcat accggaaagg tatcctgcca gcacatcaag
     1741 gcgcttttca ctgtagggcg tgagataaca acctgtaatg gcatctgcca gcgagggcaa
     1801 ttccggcgtg ttaatgtcct gcgggcattc gcgccacatt tttgccagtg ccggagcgtg
     1861 ccagccaccg cagacgacta acacgtcgcc attattttgc tgcaccgccc atgcaatcca
     1921 gcgagccata aaggcttcgc gctgacgatt gagtgcatcg ccggggaaat cgccccgcag
     1981 ttgggcaaaa tagtgcgcca gcgcagaggg taatgcagtt tgctggcttt catcttcgaa
     2041 caagtgatcc cacagggtat cgctgttatc catgcgggtg gcacgcagca gtaaggcctg
     2101 gctttcatct tgcgtatcag gcgaatcgtc ctcttcttca ctctgcgccc agcaaggcaa
     2161 atcgatgaag taagtttgtg cctgaatacg acgtgcggct tgtagcgcct gccactccgg
     2221 cgaaaattca gcaaatggcg tccaggcacc acgcccgggg gctgcaccgt cctgatactg
     2281 gcaataactg taaatagcta ccggaagctg gtgggaaaaa aaaagttcgt ctacccggtc
     2341 attaaaatca gccgggcctt caatcaacac gtatcgtggc cgctggcttt cgattaacga
     2401 tttcaccagc cgggcgcagg ccggactatg atgccggatg ccgacaatta acggctcgct
     2461 catgcatctt tcctcacggc aggcggtggc gggcttgata ataagcctgc cagtgagctt
     2521 ctttatgtgt ggcgactcgt tgttcgaagt aacggcgcag acgagcgcga tcttcttcgt
     2581 tatctttgac gatggttccg gcaatgcagt ccaccaggtc ggctggctca cctgcgcgat
     2641 tcgctaaaaa ccaggcgcgg acgcctacgg cgtgggccac gttcacggct tcagcggtgg
     2701 acataatcgc tgttagtgta tccattgacg ttttcttttc gccgttggca cgcagatcgc
     2761 ggaaggtgcg taccagtaac tccagtactg cgtcaggcac tttatgcgga ataccgctat
     2821 gggccagcaa acgcgccgaa gcactggcga ccagttccag ctcctgggcg aaatccataa
     2881 tcggaaagac ggtttcaaaa tcgaagcgac gctttagcgc ggcactcatc tcattgacgc
     2941 cgcgatcgcg ggtattggca gtggcaataa tattaaagcc ttcccgcgca tagagctgac
     3001 tggcttcacc ggtgagttcc ggcaccgtca tcaccctgtc agaaagcata cctaacagac
     3061 agtcttgtac ttccagcggt gtacgcgtta tctcttcaaa acggacgatt ttgccgtcgc
     3121 gcattccctg gtaaagcggg gcagggacca gtgcttccgt tgacggacca tggttgataa
     3181 gcagggcgta gttccagccg tacttgattt gatcttcggt agtagatgcg ccgccctgga
     3241 tggttaaccc ggcatcgccg ctaatagcgg ttgccagtaa ttcagacagg agagattttg
     3301 cggtgccagg ttcccccacc aacatcaaac cgcgcccggt ggcaagagtg actaacatgc
     3361 gttcgacagc ggagggacta atcaccactt tacggctaat attttgcgcg ctatcgccaa
     3421 ggataaaagc acgggccgca ggcaaactta actgccaacc gggcgggcaa ggagcgttat
     3481 cattttgttt taattttgcc agttcatcgg cgtataacac agcagcaggc ggacgctgca
     3541 gatgattatt ctgcggtgac atatattcat ccctgaaatg tattaatgaa ataaacgata
     3601 ttttacgcgc aataaatgtt tggcgcatta gttgtttgag caaaaaatat ctggaaagca
     3661 gatcgcagta aataaaaaaa cctgctggtg aggcaggttc ttttatttac tattttcagg
     3721 cctaacacca cagcctgtgc aacatcgtgt tccaacgaac gaattataac cacggctgga
     3781 ccccgttgca attaagataa tgagatttat aatccagacc gttgggacgt tgcgccggat
     3841 cggaccaatt tgaattattt agagtatatt attctatttc ttggttgaat atattctgta
     3901 gttcacgatt aaaaattgct attacagttt caggtgattc acctcgtaat ttccgctcat
     3961 caatacactc atctcttata taaatcaacg catcatttat tagcgggtca gcatcttctc
     4021 gatcacgttg atgaaatgtt ggacgtagcc ggaatcgctc cagtcgatag acggaacctg
     4081 taaataattc agagcattta tggatctcaa gtctgaagga aaacggatct tcaggtacag
     4141 ggacctcata atcgtaaata gcaaccagct ctttttgcac gatcatcgct ttccccgctg
     4201 gtaagtcgtg ttgataacgg gcagttgcat cactgcccgc tgtgttttaa tggaaaatga
     4261 cgtcaggtgc gcttaacgcc tcactcaaat caaacacatc aagttcggca aaagtgacgg
     4321 atttattgtc actgtacact cgaagagaac ggaagctcat tagcgctttg ctatcaacat
     4381 tgtagtggtt aaaaccgggt gaaaacttaa ggaccagccg gtactcaccc aggtcataga
     4441 caaatgcgtc ctcatagcct gactcccagc ctttgtatcg cattcccata agttgcccaa
     4501 cggtagcaga tttaccttcc cagcgattca aactattact ggctgactcg tcaggcgtga
     4561 gcagcaccgt gcgccgtgtc aactggcgaa gtggcggtat aatttcgtaa tcggcaaaaa
     4621 gctgggcaaa ttcactgcgc atttctgctg tcatttctaa cgggtgggca atgccaatca
     4681 gagcatccgc aggcaggtca attggctcat cctgcgcatt gcagaactcc ccctctgcgg
     4741 ccacacgaaa ggcgttgagt aaacgacgcg gttcatttgc cggatacacg ccccatatta
     4801 atcgctgggt aaccaggcgg gtaaagggat gattaacaaa cagagtatga aaatctgcca
     4861 gcgaccagcg tcgggtggta cgtagggcag cttcaagacg cgggagcagg tttttgctca
     4921 cctgagtagc atcttttttt agccctttta gtcgggccag tgcctcgggc gctttcagtt
     4981 gatcgtcatc ggcgcgcaac cggggaacgc ttttctggcg aacgttttgc tgatcaaaga
     5041 taaccgggtt gaggttttca tcgaagcgaa cggtaaactg ccggggacca aaatcaaaac
     5101 tcaacgcctg cggatcatca aggcccaggg ttgggactaa tctgtccgct agctcttcct
     5161 ggcttagccc gcgattttct gcgaccacct gaagaaattc cgccgcgtta tcacgtaagg
     5221 ggcgggattt agcccgttgc gatatatgat gcagctgtat cagcgccata tcattattca
     5281 tcagggtaag gatgttcagg ccgctgacag cacgggcaga tttgccttct tgtggccagg
     5341 cgaggatctg tgtcgtcaga tcccgtgccg tgctttcgtc accaaagaga cttaacgcca
     5401 gaaatgccca gttgtctttt gcgggggcac cagcttgttg ccacgcagtg aagagatccc
     5461 aggcaaaagc tgccagcgtt tgtggctggc agaacgtttt cagttgttcc agcccgctat
     5521 aaaaacgtcc cccctgggta aagcgcagca tttcgccgat aatttccaga gcgtcaccgg
     5581 taacaggctg attattagtg ataagtcgag gcctggacca cattgccgga tgccagaaat
     5641 caggggcttt tggaatgcgt gccgggtaaa tgtccattgg accctgtata ccctgttcct
     5701 ggtggcagaa ttgttcttaa tgttcaaatt tgcacgcctc ggcccaagca gcctgaaaac
     5761 cggtcgctat cactttgagc agtcttccac gactactcag ccggcacgct aagacaggag
     5821 tcgtcaaatg atcgattatg aagtattgcg ttttatctgg tggctgctgg ttggcgttct
     5881 gctgattggt tttgcagtca ctgacggttt cgacatgggg gtgggcatgc tcacccgttt
     5941 cctcggtcgt aacgacaccg agcgtcgaat tatgattaac tccattgcac cacactggga
     6001 cggtaaccag gtttggctga tcaccgcggg cggcgcactc tttgctgcct ggccgatggt
     6061 ctatgccgct gcgttctccg gcttctatgt ggcgatgatc ctcgtgctgg cgtctttgtt
     6121 cttccgtccg gtcggttttg actaccgctc caagattgaa gaaacccgct ggcgtaacat
     6181 gtgggactgg ggcatcttca ttggtagctt cgttccgccg ctggtaattg gtgtagcgtt
     6241 cggtaacctg ttgcagggcg taccgttcaa cgttgatgaa tatctgcgtc tgtactacac
     6301 cggtaacttc ttccagttgc ttaacccgtt cggcctgctg gcaggcgtgg tgagcgtagg
     6361 gatgatcatt actcagggcg caacctatct gcaaatgcgt accgtgggcg aactgcacct
     6421 gcgtacccgt gcaacggctc aggtggctgc gctggtgaca ctggtctgtt tcgcactggc
     6481 tggcgtatgg gtgatgtacg gtatcgatgg ttatgtcgtg aaatcgacaa tggaccatta
     6541 cgcagcctct aacccactga ataaagaagt ggttcgtgaa gctggcgcat ggctggttaa
     6601 cttcaacaac acgccaattc tgtgggctat tccggcactg ggtgtggttc tgccgctgct
     6661 gaccatcctg actgcacgta tggataaagc cgcgtgggcg tttgtgttct cctccctgac
     6721 gctggcctgc atcatcctga cagccggtat cgcaatgttc ccgtttgtga tgccgtccag
     6781 caccatgatg aacgcaagtc tgacaatgtg ggatgcaact tccagccagc tgacgcttaa
     6841 cgtcatgacc tgggttgcgg tggttctggt accgatcatt ctgctctaca ccgcctggtg
     6901 ttactggaaa atgttcggtc gtatcaccaa agaagatatt gaacgtaaca cccactctct
     6961 gtactaagta aggagctaaa aatgtggtat ttcgcatgga ttctgggaac gcttcttgcc
     7021 tgttcgtttg gggtaatcac cgcgctggcg cttgaacacg tcgaatcagg caaagccggt
     7081 caagaagaca tctgatgagt aagattatcg cgactttgta tgcggtaatg gacaagcgcc
     7141 ccctgcgggc gctttccttc gtgatggcgc ttctgttagc aggatgtatg ttttgggacc
     7201 catctcgttt tgccgcgaag accagtgaac tggaaatctg gcatggttta ttgctgatgt
     7261 gggccgtctg tgctggtgtg attcacggcg tgggctttcg tccgcagaag gttctttggc
     7321 aagggatttt ttgcccattg cttgccgata ttgttctcat tgtcgggctg attttcttct
     7381 tcttttaaat cagaattctc tttaaaaaat tatgggccgc tccaggccca taaattttta
     7441 cgctccctta acttgccctc attcccaaac ctcaatcgcg cgcgtatagt agcagcgttt
     7501 aaaagctcta acttttgttg cattaccggg atgtaaagtg aatacaacgc tgtttcgatg
     7561 gccggttcgc gtctactatg aagataccga tgccggtggt gtggtgtacc acgccagtta
     7621 cgtcgctttt tatgaaagag cacgcacaga gatgctgcgt catcatcact tcagtcagca
     7681 ggcgctgatg gctgaacgcg ttgcctttgt ggtacgtaaa atgacggtgg aatattacgc
     7741 acctgcgcgg ctcgacgata tgctcgaaat acagactgaa ataacatcaa tgcgtggcac
     7801 ctctttggtt ttcacgcaac gtattgtcaa cgccgagaat actttgctga atgaagcaga
     7861 ggttctggtt gtttgcgttg acccactcaa aatgaagcct cgtgcgcttc ccaagtctat
     7921 tgtcgcggag tttaagcagt gactgacatg aatatccttg atttgttcct gaaggctagc
     7981 cttctggtta aacttatcat gttgattttg attggttttt caatcgcatc ttgggccatt
     8041 attatccagc ggacccgtat tcttaacgca gcggcgcgcg aagccgaagc gtttgaagat
     8101 aaattctggt ctggaatcga actctctcgc ctctatcaag agagccaggg gaaacgggat
     8161 aatctgactg gttcggaaca aatcttttac agcgggttca aagagtttgt gcgcctgcat
     8221 cgtgccaata gccatgcgcc ggaagccgta gtggaagggg cgtcgcgtgc tatgcgtatc
     8281 tccatgaacc gtgaacttga aaatctggaa acgcacattc cgttcctcgg tacggttggc
     8341 tccatcagcc cgtat
//
LOCUS       DAMZET010000176         8303 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.176, whole genome shotgun sequence.
ACCESSION   DAMZET010000176 DAMZET010000000
VERSION     DAMZET010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.176"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..628)
                     /gene="apbC"
                     /locus_tag="POB34_004001"
     CDS             complement(<1..628)
                     /gene="apbC"
                     /locus_tag="POB34_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HDI5920860.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQ"
     gene            760..2793
                     /gene="metG"
                     /locus_tag="POB34_004002"
     CDS             760..2793
                     /gene="metG"
                     /locus_tag="POB34_004002"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDI5920861.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2934..6728
                     /locus_tag="POB34_004003"
     CDS             2934..6728
                     /locus_tag="POB34_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HDI5920862.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQDNELHISWGKIGTHGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDIEWDYANNVIIILPSGIAPSYLEQYSRFELR
                     LRKHLSLTEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKAIAAFPHAAMAALTELLGQK
                     EENSWRIMLMTMLISQPALAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAVV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPICYLTEEISNQLLAKYIWYSKHITVSHEESTT
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLLSEFKVFHSPTGHYWQLGILTTL
                     PLEKAVKAWNALTLSPHTDTEYAMLHFGLKGLPGLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRENARSWLLKYPEHALTGLLPAALGKAGEAQDNARAALRMLTEN
                     GHQPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKAN
                     AQSLPDSALLHLGEMLRFPQEEALYPGLLQVKDVCSADSLAGFAWDLFTAWQTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDVSGSRLKDLPKPNKSDDETRANDAVNRYKLLKKDARTIAAQQVAR
                     LESAMCLRRRWSLENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYST
                     ADDDLFTLPEGDISIGTPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAE
                     RNASELTRWAGRKCPSGRVMGLANKGWIKGEPQDGGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEAL
                     FE"
     gene            6738..8303
                     /locus_tag="POB34_004004"
                     /pseudo
     CDS             6738..8303
                     /locus_tag="POB34_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2178 a         2155 c         2052 g         1918 t
ORIGIN      
        1 cctgcaacat ctgcattaac gccttgctcg ccatcggtcc acgccacacc atcgcattgt
       61 cgtcagtgac cagataacca attgaattgg ttgccaggcc atgagacata atcggtgcca
      121 tatgggtacc gtccggcgaa gttggacgct gattttctgc gcccagcatg gtgggaattg
      181 atgggccgta gatatcggcg tccagaatgc cgactttcgc cccttccgcc gccagtgcca
      241 gcgccaggtt taccgccgtg gaggatttac ccacgccgcc tttaccggaa ctgaccgcaa
      301 tgatgttctt cacaccatta atacccggct gattcttcac gcgttttaag gtggcgatat
      361 tgtgcgacag tttccagtcg atagctttcg ccccggtgat acgcagcagt tcggcgctac
      421 attgctcttt taactcttca aacgcgctat gccagacgaa tggcataacc agttccacat
      481 gcaacgtgtc gtccatccag gcgacatggt gcaacgcttt cagcgtggtg aggttatgct
      541 tcagggttgg gtgctgaaaa ttggccagtg tcccggcgac cattgcgcgc aaggcttctg
      601 gtgatttggc ctgggattgt tcgttcatcc cgactccttt tttgtataga taaaccatca
      661 gctgatagtt tacctgaaga atatagagaa gtacttactt aacattttcc catttggtac
      721 tatctaaccc cttttcacta ttaagaagta atgcctacta tgactcaagt cgcgaagaaa
      781 attctggtga cgtgcgcact gccgtacgct aacggctcaa tccacctcgg ccatatgctg
      841 gagcacatcc aggctgatgt ctgggtccgt taccagcgaa tgcgcggcca cgaggtcaac
      901 ttcatctgcg ccgacgatgc ccacggtaca ccgatcatgc tgaaagctca gcagcttggt
      961 atcaccccgg agcagatgat tggcgaaatg agtcaggagc atcagactga tttcgcaggc
     1021 tttaacatca gctatgacaa ctatcactcg acgcacagcg aagagaaccg ccagttgtca
     1081 gaacttatct actctcgcct gaaagagaac ggttttatta aaaaccgcac catctctcag
     1141 ctgtacgatc cggaaaaagg catgttcctg ccggaccgtt ttgtgaaagg cacctgcccg
     1201 aaatgtaaat ccccggatca atacggcgat aactgcgaag tctgcggcgc gacctacagc
     1261 ccgactgaat tgatcgagcc gaaatcggtg gtttctggcg ctacgccggt aatgcgtgat
     1321 tctgaacact tcttctttga tctgccctct ttcagcgaaa tgttgcaggc atggacccgc
     1381 agcggtgcgt tgcaggagca ggtggcaaat aaaatgcagg agtggtttga atccggcctg
     1441 caacagtggg atatctcccg cgacgcgcca tactttggtt ttgaaattcc gaacgcgccg
     1501 ggcaaatatt tctacgtctg gctggacgca ccgattggct acatgggttc tttcaagaat
     1561 ctgtgcgaca agcgcggcga cagcgtaagc ttcgatgaat actggaagaa agactccacc
     1621 gccgagctgt accacttcat cggtaaagat attgtttact tccacagcct gttctggcct
     1681 gccatgctgg aaggcagcaa cttccgcaag ccgaccaacc tgtttgttca tggctatgtg
     1741 acggtgaacg gcgcaaagat gtccaagtct cgcggcacct ttattaaagc cagcacctgg
     1801 ctgaatcatt ttgacgctga cagcctgcgt tactactaca ctgcgaaact ctcttcgcgc
     1861 attgatgata tcgatctcaa cctggaggat ttcgttcagc gtgtgaatgc cgatatcgtg
     1921 aacaaagtgg ttaacctggc ctctcgtaat gcgggcttta tcaacaagcg ttttgacggc
     1981 gtgctggcaa gcgaactggc tgacccgcag ttgtacaaaa ccttcactga tgccgctgaa
     2041 gtgattggtg aagcgtggga aagccgcgaa tttggtaaag cagtgcgcga aatcatggcg
     2101 ctggctgatc tggctaaccg ctatgtcgac gagcaggctc cgtgggtggt ggcaaaacag
     2161 gaaggccgcg atgccgacct gcaggcaatt tgctcaatgg gcataaacct gttccgcgtg
     2221 ctgatgactt acctgaagcc ggtactgccg aaactgaccg agcgcgcaga agcattcctc
     2281 aatacagaac tgacctggga tggtatccag caaccgctgc tgggccacaa agtgaatccg
     2341 ttcaaagcgc tgtataaccg catcgatatg aagcaggttg aagcactggt ggaagcgtct
     2401 aaagaagaag tgaaagcagc tgccgcgcca gttactggcc cactggcaga tgatccgatt
     2461 caggaaacca tcacctttga cgacttcgca aaagttgacc tgcgcgtggc gctgatcgaa
     2521 aacgcagagt ttgttgaagg ttctgacaaa ctgctgcgcc tgacactgga tctcggcggt
     2581 gaaaaacgca atgtcttctc cggtattcgt tctgcctacc cggatccgca ggcactgatc
     2641 gggcgtcaca ccattatggt ggccaacctg gcaccgcgta aaatgcgctt tggtatctcc
     2701 gaaggcatgg taatggctgc tggtcctggc gggaaagata tcttcctgtt aagcccggat
     2761 gctggtgcta aaccaggcca tcaggtgaaa taatccccct ttaaggcgct gcatcgacag
     2821 cgccttttct ttataaattc ctaaagttgt tttcttgcga ttttgtctct ctctaacccg
     2881 cataaatact ggtagcatct gcattcaact ggataaaatt acagggatgc agaatgagac
     2941 actttatcta tcaggacgaa aaatcacata aattctgggc agttgagcaa caggataatg
     3001 agcttcatat tagctggggc aaaatcggta cccacggaca aagtcagata aaaagttttt
     3061 cagatgctgc ggcagcggca aaagcggagc ttaagctgat tgcggagaag gtgaagaagg
     3121 ggtatgtgga gcaagcgaag gataattctt tgcaaccttc ccaaacggta acgggctctc
     3181 tcaaggtagc ggacttatcc accattattc aggaacaacc ctctttcgta gcagaaaccc
     3241 gtgcgcctga caaaaataca gatgctgttt taccgtggct ggcgaaagat attgctgtcg
     3301 tttttccgcc cgaagttgta cacaccacgt taagtcatcg ccgctttccc ggagttcctg
     3361 ttcagcaagc agacaaattg acccaattac gtcgcttagc ctgtagtgtg tcgcaacggg
     3421 ataataaaac agccacattt gacttcagcg cctgttcttt agaatggcaa aacaccgtcg
     3481 cccaggcgat cagtcagatc gacggcctga aaacaacaca gttaccatca ccagtaatgg
     3541 ctgtactcac ggcacttgaa atgaaatgca caagatataa agtgcgtgag gatgttatgg
     3601 atcagatcgt ccaggaaggc ggtctggaat atgctactga tgtaataata caccttcaac
     3661 agattgatat tgaatgggat tatgcgaata atgtcattat tattctgccg tctggcattg
     3721 cacctagcta cttggagcaa tattccagat ttgaattacg cctacgtaaa catttatcac
     3781 tgacggaaga gtctctctgg caaaaatgtg cacaaaaact tattgccgca attccacata
     3841 ttccagaatg gcggcaacca ttaattgctt tgttattacc cgaaaaacca gaaattgcac
     3901 atgaaattgc ccagcgtcta ctggggcaaa aaaaattacc ctcgcttgag tggttaaaaa
     3961 tagtggcgac tgatgagcac attcttgcct cattagaaaa atatcacgaa ccatatgcca
     4021 tttttgatga ttactattgt ggtgcgatat ggtcagccac cgtattacag gagcaaggtg
     4081 ttgcagccct gccccgattt gctccctatg ccgcaagtga ctactgcgcc gatgtgttgc
     4141 gtcatatcaa tcatccgttc gcattaacac tgcttatacg tgtagccggg catactaaac
     4201 gctgtcacga tcggatgacg aaagccattg ctgcgttccc acatgcagca atggcggcac
     4261 tgacggaact tcttgggcaa aaagaagaga acagttggcg cattatgcta atgacaatgc
     4321 ttatctcaca accagcactg gcagaacagg tcattccctg gctctcgaca cccgcagttg
     4381 ccgtactgaa atcatgccag caacaactga cacagccctc aaaccatgcc agcgccgatc
     4441 tactgccagc cgtagtagtc tcccctccct ggctttcgaa aaagaaaaaa tcgccgattc
     4501 cggtgctgga tttagcgcca ttaggcattg agccaatctg ttatctgaca gaagaaatca
     4561 gtaatcaact tttggcgaaa tatatctggt attcaaaaca catcacggtt agccatgaag
     4621 aaagtactac caacctgttg gcaaggatgg gttttcaacg acggatcgct ggtacatata
     4681 ttaaagctcc cgaagcggta gttgaggcat ggctaaatga agattattca accttactaa
     4741 gtgaatttaa ggtgtttcat tcacctaccg ggcattattg gcagttgggg attttgacaa
     4801 cattgccgct ggagaaagca gtaaaagcat ggaatgccct taccctatct ccacataccg
     4861 ataccgaata cgccatgtta cattttggac tcaaagggtt acctgggtta gtaaactcac
     4921 ttgcacgcta tccacaagaa gccttgccca tcacgaatta cttcgcagcg agtgagctgg
     4981 ctcctgccgt cgcccgtgcc ttcaacaaac tgaaaacgct acgcgaaaac gcccgtagct
     5041 ggctattgaa atacccggaa catgccctta ccggcctgct gcctgcggcg ctcggcaaag
     5101 ccggtgaagc acaggataac gcccgcgctg ccttgcgtat gcttaccgaa aacggtcatc
     5161 agccattact gcaagaaatc gcccgacgtt ataaccagcc ggaagtaacc gatgcggtga
     5221 acgctctgct tgcgctcgat cccttagata atcacccgac aaaaatcccc actcttccgg
     5281 ccttttatca gccatcgctc tggacgcgcc cggtattaaa agcaaatgcc caatcactgc
     5341 cagatagcgc cctcctccac ctcggtgaaa tgctccgctt ccctcaggaa gaggctctgt
     5401 atccgggatt attgcaggtg aaagacgtct gttccgccga ctcactggcg ggatttgcct
     5461 gggatctgtt taccgcctgg cagaccgctg gcgcgccgtc gaaagagagt tgggcgttca
     5521 ctgcgttagg cgttctcggt aacgatgaca ccgcccgcaa actgacgcca ttaatacgcg
     5581 cctggcctgg tgaatcccag cataaacgcg ccaccgttgg gttggatatt ctcgctgcta
     5641 tcggtagtga tatcgccctt atgcagctta acggcatcgc ccagaaactg aaattcaaag
     5701 cattacagga gcgggcaaaa gaaaaaattg ccgacattgc cgagagccgc gaactcacgg
     5761 tggcggagct tgaagatcgg ttagcaccgg atctcggtct ggatgataac ggttcgctgc
     5821 tgctggattt tggcccacgg cagttcaccg tcagctttga tgaaacctta aaaccgtttg
     5881 tgcgtgatgt ttccggcagc cgcctgaaag acctgcccaa accgaacaaa agcgatgatg
     5941 aaacgcgggc gaacgatgcg gttaaccgct acaaattgct gaaaaaagat gcgcgtacca
     6001 tcgccgccca gcaggtagca aggctggaat ccgccatgtg cctgcgccgc cgctggtcgc
     6061 tggaaaactt ccagctcttc ctggttgagc atccgctggt tcgtcactta acccgccgtc
     6121 tgatttgggg cgtttatagc gccgaaaacc agctactggc ttgctttcgc gtagcagaag
     6181 ataacagcta cagcaccgct gacgatgatc ttttcaccct gccggaaggc gatatctcta
     6241 tcggcactcc tcacgttctg gaaatatcac caacggatgc tgccgccttt ggtcagcttt
     6301 ttgccgacta cgaactgcta ccaccgttcc gccagctcga ccgtaacagc tacgccctga
     6361 cagaagccga gcgcaatgcc agtgaactga cccgctgggc aggcagaaaa tgcccgagtg
     6421 gtcgggtcat ggggctggcg aataaaggct ggataaaggg cgaaccacag gatggaggct
     6481 ggatcggatg gatgatcaaa cctttgggtc gctggtcgtt aatcatggaa atcgacgaag
     6541 gttttgcggt gggtatgtca ccagccgaac ttagcgctga gcagctctta agcaagctgt
     6601 ggttatggga aggcaaagcg gaaagctatg gctgggggag taattcaaca caggaagcgc
     6661 agttctccgt actcgatgcc atcaccgcca gcgagctaat taacgatatt gaagccctgt
     6721 ttgaataagg aaagagcatg gacaaggaat taccgtggct ggcggataac gcccaactgg
     6781 aactgaaata taaaaaaggc aaaacaccac tcagtcatcg caaatggccg ggcgaaccgg
     6841 tgcctgttat cactgaaagc atcattcaga cactgggtga taaattgcta caaaaagctg
     6901 agaagaaaaa aaacattgtc tggcgttatg aaaatttttc actggagtgg cagtccgcca
     6961 tcacgcaggc catcaacttg atcggcgaac acaaaccctc aatcccggcc cggacaatgg
     7021 cggcgctagt ctgtatcgcg caaaatgaca gccaacagtt gctcgacgaa atcgtccaac
     7081 aagagggact ggaatatgcg actgaggtgg tgattgcacg ccagtttatt acacgatgtt
     7141 atgagagtga tcctctgcta gtgacattgc agtatcagga cgaggattat ggctatggtt
     7201 atcgctcaga aacctataac gaattcgatc tccgactgcg taagcatctc tctctggcag
     7261 aggaaagcag ctggcagcgt tgcgccgaca aactcattgc cgcactgcca ggaataccca
     7321 aaattcgccg tccttttatt gcactactcc tcccggaaaa acctgaaata gccaatgaac
     7381 tcgccagtct tgaaagctcc cggtcaagtc tccattcaaa ggagtggtta aaggtcattg
     7441 ctactgataa tacggcggta aaaaaactcg aacgatactg gggcctggac gtatttagcg
     7501 atcgcgaagc cagctacatg tcacaagaaa accgcttcgg ctacgcggcc tgcgcctccc
     7561 ttttgcgcga acaaggactg gcagccgttc cgcgcctggc aatgtatgcc cataaagaag
     7621 attgcggcag tctgctggta caaattaacc atccgcaagt catccgcacc ttgctactgg
     7681 tggctgataa aaacaaaccc agcctgcaac gtgtggctaa atacagtaaa aacttccccc
     7741 acgcgacgct cgccgcactg gcagaactgc tggcgttaaa agaaccacca gcccgcccag
     7801 gttatccaat catcgaagac aaaaagctgc ctgcacagca aaaagcacgc gatgaatact
     7861 ggcgtacgct gttacaaacg ctgatggcat cgcagccaca actggcagca gaagtgatgc
     7921 cgtggttaag tactcaagcc agggcagtgc tgaatagtta tttatcggca ccgccaaaac
     7981 cggttattga tagcaccgat aacagcagtc tgcctgaaat gctcgtttca ccaccgtggc
     8041 gtagtaagaa aaaaatgaca gctccacgtc ttgatttggc accgctcgaa ttaactccgc
     8101 aaatttactg gcaaccaggc gaacaagaga ggcttgccgc cactgagtct gcccgttatt
     8161 tcagcacgga atctcttgcg gaacgcatgg aacaaaaaag tggacgagtt gtattacagt
     8221 aactgggttt tggggatgat gtatggctgt ttctgaatta tatactcccc ggaaaactgg
     8281 atgctgcacg caattcactc att
//
LOCUS       DAMZET010000177         8245 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.177, whole genome shotgun sequence.
ACCESSION   DAMZET010000177 DAMZET010000000
VERSION     DAMZET010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.177"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..251
                     /locus_tag="POB34_004005"
     CDS             <1..251
                     /locus_tag="POB34_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308745.1"
                     /note="negative modulator of the initiation of chromosome
                     replication; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation regulator SeqA"
                     /protein_id="HDI5920863.1"
                     /translation="SLDAQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGT
                     PYWVITNTNTGRKCSMIEHIMQSMQFPAELIEKVCGTI"
     gene            277..1917
                     /gene="pgm"
                     /locus_tag="POB34_004006"
     CDS             277..1917
                     /gene="pgm"
                     /locus_tag="POB34_004006"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HDI5920864.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(1973..3292)
                     /gene="potE"
                     /locus_tag="POB34_004007"
     CDS             complement(1973..3292)
                     /gene="potE"
                     /locus_tag="POB34_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HDI5920865.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(3289..5487)
                     /gene="speF"
                     /locus_tag="POB34_004008"
     CDS             complement(3289..5487)
                     /gene="speF"
                     /locus_tag="POB34_004008"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HDI5920866.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKETRPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            complement(5798..5902)
                     /locus_tag="POB34_004009"
     CDS             complement(5798..5902)
                     /locus_tag="POB34_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HDI5920867.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            complement(6177..6854)
                     /gene="kdpE"
                     /locus_tag="POB34_004010"
     CDS             complement(6177..6854)
                     /gene="kdpE"
                     /locus_tag="POB34_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HDI5920868.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFMP"
     gene            complement(6851..>8245)
                     /locus_tag="POB34_004011"
     CDS             complement(6851..>8245)
                     /locus_tag="POB34_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HDI5920869.1"
                     /translation="LYLLGVVVVALFYGRWPSVVATVINVVSFDLFFIAPRGTLAVSD
                     VQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYREQRTRHLYEMSKALAVGRSPQDIA
                     ATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQGMTPWDDAIAQWSFDKGLPAGAGTD
                     TLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMIPEQQRLLETFTLLVANALERLTLT
                     ASEEQARMASEREQIRNALLAALSHDLRTPLTVLFGQAEILTLDLASEGSPHARQASE
                     IRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLTLEEVVGSALQMLEPGLSSPINLSL
                     PEPLTLIHVDGPLFERVLINLLENAVKYAGAQAEIGIDAHVEGENLQLDVWDNGPGLP
                     PGQEQTIFDKFARGNKESAVPGVGLGLAICRAIVDVHGGTITAFNRPEGGACFRVTLP
                     QQTAPELEDFHEDM"
BASE COUNT         2144 a         2154 c         2055 g         1892 t
ORIGIN      
        1 attctcttga cgcccaggcg tttgccgaag caacggaatc gttgcacggt cgtacacgcg
       61 tttactttgc ggcagatgaa caaacgctgc tgaaaaatgg taatcagacc aagccgaaac
      121 atgtgccagg cacgccgtat tgggtgatca ccaacaccaa caccggccgt aaatgcagca
      181 tgatcgaaca catcatgcag tcgatgcaat tcccggcgga attgattgag aaggtttgcg
      241 gaactatcta aaacgttgca gacaaaggac aaagcaatgg caatccacaa tcgtgcaggc
      301 caacctgcac aacagagtga tttgattaac gtcgcccaac tgacggcgca atattatgta
      361 ctgaaaccag aagcagggaa tgcggagcac gcggtgaaat tcggtacttc cggtcaccgt
      421 ggcagtgcag cgcgccacag ctttaacgag ccgcacattc tggcgatcgc tcaggcaatt
      481 gctgaagaac gtgcgaaaaa cggcatcact ggcccttgct atgtgggtaa agatactcac
      541 gccctgtccg agcctgcgtt catttccgta ctggaagtgc tggcagcgaa cggcgttgat
      601 gtcattgtgc aggaaaacaa tggcttcact ccgacacctg ccatttccaa tgccatcctg
      661 gttcacaata aaaaaggtgg cccgctggca gacggtatcg tgattacacc gtcccataac
      721 ccgccggaag atggtggtat caagtacaac ccgccaaatg gtggcccggc tgataccaac
      781 gtcaccaaag tggtggaaga cagggccaac gcactgatgg ccgatggcct gaaaggcgtg
      841 aagcgtatct ccctcgacga agcgatggca tccggtcatg taaaagagca ggatctggtg
      901 cagccgtttg tggaaggtct ggccgatatc gttgatatgg cggcaattca gaaagcgggc
      961 ctgacgctgg gcgttgatcc gctgggcggt tccggtatcg aatactggaa gcgtattggc
     1021 gagtattaca acctcaacct gaccatcgtt aacgatcagg tcgatcaaac cttccgcttt
     1081 atgcaccttg ataaagacgg cgcgatccgt atggactgct cctccgagtg tgcgatggcg
     1141 ggcctgctgg cactgcgtga taagttcgat ctggcgtttg ctaacgaccc ggattatgac
     1201 cgtcacggta tcgtcactcc ggcaggtttg atgaatccga accactacct ggcggtggcg
     1261 atcaactacc tgttccagca tcgtccgcag tggggcaaag atgttgctgt cggtaaaacg
     1321 ctggtttctt ctgcgatgat cgaccgtgtg gtcaatgact tgggccgtaa gctggtagaa
     1381 gtaccggtag gtttcaaatg gtttgttgat ggtctgttcg acggcagctt cggttttggc
     1441 ggcgaagaga gcgcaggggc ttccttcctg cgtttcgacg gcacgccgtg gtccaccgac
     1501 aaagacggca tcatcatgtg tctgctggcg gcggaaatca ccgcggtcac cggtaagaac
     1561 ccgcaggaac actacaacga actggcaaaa cgctttggtg cgccgagcta caaccgtttg
     1621 caggcagctg cgacttccgc acaaaaagcg gcgctgtcta agctgtctcc ggaaatggtg
     1681 agcgccagca ccctggcagg cgacccgatc accgcgcgcc tgacggcggc accgggcaat
     1741 ggtgcttcta tcggcggtct gaaagtgatg actgacaacg gctggttcgc cgcgcgtccg
     1801 tcaggcacgg aagacgcata taagatctac tgcgaaagct tcctcggtga agaacatcgc
     1861 aagcagattg agaaagaagc ggttgagatt gttagcgaag ttctgaaaaa cgcgtaaaca
     1921 catttaataa aaaaagggcg gtcgcaagat cgcccttttt tactttgctt ttttaaccgt
     1981 gtttattttt cagttcaaag cgtggtgata ccagaccata cagtgtccaa ccaaggaagg
     2041 ttacgatgga accgtacagc atggcttctt ccccggatga gtacagcgca taaaagctat
     2101 acatcgcgcc aacaaaagca acaaagtttg caactttcgc ttttgagggg ggcacattag
     2161 cgaccttctg aataatgact aatgccgcca ttgacagaat atacggaatg atattggtca
     2221 ccacggccag gttaaccagc acgttgaact gactgtttag cgacgggcta atcgtcatca
     2281 gcgccaaccc gctctggata atcacaatgg tcaacattcc ctgcaccggt gcatccactt
     2341 tggttacacg ggagaaaatt ttagggaagt agccttcatc agatgaagat ttaaacacct
     2401 gggcaatggt gaactgccag ccaagcagcg aaccgcagca ggacatcacc atcagcgcca
     2461 taatgacttt acccacttct ggtgtgaaca tctgtgcgaa ggccaggcca aatggtgccg
     2521 ttgaatttgc cagctccata ttaggcacaa tccctgcaat cacgttggtg gagacgatat
     2581 aaatcaccgc cgcacctaac gtaccgccga gtaccgcgat tggcacatta cgttccgggt
     2641 tttccactac atcagtattc gcacacgcag actccagacc gagaaaagcc cacagcgtca
     2701 tagcgatgga agaacctact gcactgaaga acggtgcatg atgcggattc caggaatcaa
     2761 cgtaaagcgt cgggctaaac cagaaccagc cgataatgca caggcctacc accggaataa
     2821 tgacccccca cacagtaatg ctactgattt gcccggtaat gcgcgcacca ccaaagttag
     2881 ccacggtaca aatccacagc actccgatgg tcgcaagacc aatctgcact ggcgacaaac
     2941 tcgcgccgag cagttcggtg ccgtaaccaa ccgccgaaat agcaatcgcg acgttagcaa
     3001 tcagcagcga gacaccgtag gtgtagttcg ccataaaatt gccggactta ccgaaagcat
     3061 actcggcata accgcccata ccacctgatt tacggctgaa cataccgcat tttgcgaatg
     3121 cccatgccag tgccattgag cctacggctg tcaccagcca ggagataatt gaaattgtcc
     3181 caacttcggc aagctttgtc ggcagcatga tgataccgga gcccatcatg ttgaccatcg
     3241 tcagtatggt taactgaacg acgcccattt tgttcgattt agcctgactc ataatttttc
     3301 ccctttcaac agggtgcttt gcgcatcacg aggcttgatg acatagcacc aaacttgctt
     3361 acgaccatca tgttcttcga tatagacacc ctgaagctcc ggtgcaaaac ctggcaacag
     3421 gttgatccct tcttccagag cgctgaagta acgcagaaca gcaccacccc agatttcacc
     3481 cggaacaacg cacagcacac ccggaggata aggaagcgca ccttcggcag cgatacggcc
     3541 ttctgcatcc ggcagacgaa ccagctccac ttcaccgcgt aaataggcgt agttggcttc
     3601 ttgcggattc atgcttacgc gtgggaagtg ttccttacgg aacatctctt tttgcagttg
     3661 tttcacgttg tgacgggcat acaaatcatg catttcttga cacaactgac gcagggtata
     3721 accggcgtag cgctcttcat gctgtttgta gatggaaggc agcacttctg ctaatggcgc
     3781 gtcggactca agcagttttt cgaagcgtac cagcagggca acaagttgct gaagcttggc
     3841 catatcttct gccggagtca gcaggaagag gatggagtta agatcgcatt tttccggcac
     3901 tacgccattt tcgcgcagga agttagcaag aatcgtcgcg ggtacaccga acgcttcata
     3961 ttcgccgtta cgtgcatcaa tacctggggt tgtcagcaac agtttgcaag gatcgacaaa
     4021 gtattgattc tctgcatagc cttcaaaaga atgccagtgt tcccctggta caaactggaa
     4081 gaagcgcaga tcaaccgcaa tttgcgctgt ttcatacgac tgccagggtt taccatccac
     4141 cagttcaggt acgaacggac gaatatgctg acagttatcg aggatcagtt tgcgggcatt
     4201 aatgccattt accacacaat ccatccacat attacgaccg ctgacacctt catgcatttt
     4261 ggcgttgata tccagtgcgg caaacagcgg atagaacggg ctggtggagg cgtgcatcat
     4321 aaaggcgttg ttcatgcgtt tgtgcggtac ataacgctgt tgccctttga tgtggctgtc
     4381 ttttttatgg atttgtgaag tttgagaaaa accagcctgc tgtttatgta cggattgcgt
     4441 aaccagaata cccggatcgt tctcattaag atccagcaac agcggcgaac agtcagccat
     4501 catcggaata aactgttcat agccgaccca tgcggagtca aagaggatgt agtcacacag
     4561 atgaccaatt ttatccacca cctgacgggc gttataaatc gtaccgtcgt aagtgcctag
     4621 ctgaataacg gcgaggcgga aaggacgagt ctcttttgcc cgctgcggtg caacttctgt
     4681 aatcagctca cgcaggtaac tttcttcaaa acagtgcgca tcaatgccac cgataaagcc
     4741 atacgggtta cgtgcggttt ccagataaac cggtgtcgcg ccagcctgta gcaacgcacc
     4801 gtggtggtta gatttatggt tattgcggtc aaacaacacc agatcgcccg gcgtcagcaa
     4861 tgcgtttaag accactttat tagaagatga agtaccgttt aaaacgaaat aggttttatc
     4921 ggcattaaag actttagccg catgctgttg tgcaatacat ggcgcacctt catgaatcag
     4981 cagatcgccc attgctacgt cagcattaca caagtcagag cggaagagca tttcgccaaa
     5041 gtattccacg aactgattgc ctgccggatg gcgacggaaa aactcacccc cctgatgacc
     5101 agggcagtca aaagcactat tgccctgatt aacataatcg accagtgcgc ggaagaaggg
     5161 cgggcgcagt tgcgtttcat aatggctggc agcggtttct aactgacgtc cataaaactc
     5221 tttacgtgat tcgcaatgtt cgaagacacc agaaatacgt ggcagatatt ctgcgggtac
     5281 acgttcttca ttttccgttg caataaaaac aggaatgtca tagccagtag cgtctatttc
     5341 atcgagtttt ccacgttcaa catcactgac tgataaaata atggcggcaa cgtcaatata
     5401 attactttcg tctatattca cgcatgcacg atgagtggaa aaacaatccg ggcaagaacg
     5461 actaacggca atttttaatt ctgacatttt gttcatctct ttatttcagg taatagcagg
     5521 tcctcaattt ctcaaaggag aaatcgatca cgccggaaaa aaagcaaagc tccaccgctg
     5581 ataaataaat cagtagtttg ctttttgcag atgcaataac ccagaccagc ccggtaactg
     5641 gctatactcg tcatacctca agttgcaagt gctgcgactc gaattattta gagtttaaaa
     5701 gggttagcag tatatgagca ccgaggaata caggtattaa cctgtaatgc ttaaagtcag
     5761 ggagacctgt acgttggggt gccctttagt cagaagacta gcgggcatta aagaaatgaa
     5821 agtcaaaact attacgatgg gcaaacttca tgatatgggt tgtccgcctt atatgaggca
     5881 ttaagcggtt attattttcc atttaccttt acctcttgct gtgtcagttg aaggtagagt
     5941 tattctatcc atgaaatggc tataatctgt gaagaagatc aacaaaaaac gatcataccc
     6001 caaataataa atcattaatt atgaatagcg ccatattaga tgctgttttg ttatttaaat
     6061 gttgtaattt tgtgttttat atttgcgtta agaaattaaa cctgccaaat aataatattt
     6121 ttggcaggtt taataataaa ttaataatta tttaagcgca tttattgaat aaacattcat
     6181 ggcataaacc gatacccaat accggtttca gtaatgaaat ggcgggggcg agcgggatcc
     6241 tgttccagtt tttgtcgcag atgtcccata taaatacgca aatagtgact gtgttcgacc
     6301 gcgtttggcc cccacacctg attgagtaac tggcgctggg tgagcacttt cccggcatta
     6361 ttcagcagca ccgccagcag gcggaactca atcggcgtga gatgcacctc ttcctcaccc
     6421 cggtgaatca cgcgggcggc taaatcgacg gtaacatcgg aaaatttcac cagcggatcg
     6481 ggcgcggcgg tggcggagtg gcggcgtaaa gcgacgcgca gacgggcctg caattcgcca
     6541 atgccaaacg gcttactcag ataatcatcc gctccggcat ccagcgcggc gattttgtcg
     6601 ctctcttcgc tgcgtgcgga aagcacaatc accggcaccg cgctccactg gcgcaggtcg
     6661 cggataaact caatcccatc accatcgggc aggccgagat cgagaataat caaatctggc
     6721 ttacgggtag ccgcttccag caagccgcgt tgcagcgttt cggcctcata gacgcgcatc
     6781 ccgtcgccct ccagcgccgt gcgcagaaag cgacgaatag cctgttcatc ttcaacaatc
     6841 agaacgtttg tcacatatcc tcatgaaagt cttcaagttc aggagcggtt tgctggggaa
     6901 gtgtaacacg aaaacaggca ccaccttccg gtcggttgaa cgcggtaata gtgcccccgt
     6961 gtacatccac tatcgcccga caaattgcca gtccaagccc tacccccggt actgccgact
     7021 ctttattccc gcgagcaaac ttatcaaata tcgtctgctc ctggcctggc ggaagaccgg
     7081 ggccgttatc ccagacatcc agttgtagat tttcgccctc aacgtgggca tcgataccaa
     7141 tttcggcctg cgcacccgca tatttcaccg cgttctccag cagattaatc agcacccgtt
     7201 caaagagtgg cccgtcaacg tggattaagg tcagcggttc tggcagagaa agattgatgg
     7261 gcgacgataa acccggttcc agcatttgca gcgcgctacc gaccacttct tccagcgtta
     7321 accactcttt cttcaaatta aagccgccgg actgaattcg cgccatatcc agtagattat
     7381 tcaccagtcg ggtagtgttc agcacatgct gacgaatctc gctggcctgg cgggcgtggg
     7441 gcgatccttc gcttgccaga tcgagcgtta agatttctgc ctgaccaaac agcaccgtaa
     7501 gcggcgtgcg taaatcatgc gaaagcgccg ccagcagggc gttgcggatc tgttcacgtt
     7561 cgcttgccat ccgcgcctgt tcttcgctgg cggtcagtgt cagccgctca agggcattgg
     7621 cgactaacag cgtaaatgtc tccagcaggc gctgctgctc cgggatcatc aactggcgca
     7681 gattccccgg ttccaccacc accagtccgt aggttttctc gccgcttttt agcggcaaaa
     7741 tctggtacgg cacgccgggt aacgtgtcgg tgcccgcacc cgcaggcagg cctttatcaa
     7801 aactccactg cgcgatggca tcgtcccatg gcgtcattcc ttgcggatgt gttaacggct
     7861 gcaatttacc gttgtcatcg ggcaacaaca cctgactgcg ggcatgaaac gtggaggcaa
     7921 taaattgttc gctggtggcg gcgatgtcct gcggactgcg gcccaccgcc agagctttcg
     7981 acatttcata taagtgccgt gtgcgttgct cgcggtaacg ggctacccgc gcctgataac
     8041 gcacgccagc agtaaggttc ccgatcacca gcccgacggt taacatcacc gcgaaggtca
     8101 gcagatattg cacatcagag acggcgagcg tgccgcgtgg ggcgataaaa aagagatcga
     8161 aactcaccac attaatgacg gtagcaacca ccgaaggcca gcgtccataa aatagcgcca
     8221 ctaccaccac gccaagcaga tacag
//
LOCUS       DAMZET010000178         8152 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.178, whole genome shotgun sequence.
ACCESSION   DAMZET010000178 DAMZET010000000
VERSION     DAMZET010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.178"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..198)
                     /locus_tag="POB34_004012"
     CDS             complement(<1..198)
                     /locus_tag="POB34_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HDI5920870.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWV"
     gene            728..1000
                     /locus_tag="POB34_004013"
     CDS             728..1000
                     /locus_tag="POB34_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HDI5920871.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(1001..1873)
                     /gene="rluF"
                     /locus_tag="POB34_004014"
     CDS             complement(1001..1873)
                     /gene="rluF"
                     /locus_tag="POB34_004014"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HDI5920872.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEVKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            2221..3168
                     /locus_tag="POB34_004015"
     CDS             2221..3168
                     /locus_tag="POB34_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDI5920873.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELRIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            3238..4044
                     /locus_tag="POB34_004016"
     CDS             3238..4044
                     /locus_tag="POB34_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI5920874.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSKRASYITGVTTNIAGGKTRG"
     gene            4054..4461
                     /locus_tag="POB34_004017"
     CDS             4054..4461
                     /locus_tag="POB34_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HDI5920875.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            4461..4955
                     /locus_tag="POB34_004018"
     CDS             4461..4955
                     /locus_tag="POB34_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HDI5920876.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            5021..5818
                     /locus_tag="POB34_004019"
     CDS             5021..5818
                     /locus_tag="POB34_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDI5920877.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQPQATTSTALDQLDD"
     gene            5829..6653
                     /locus_tag="POB34_004020"
     CDS             5829..6653
                     /locus_tag="POB34_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDI5920878.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            6705..7934
                     /gene="sorE"
                     /locus_tag="POB34_004021"
     CDS             6705..7934
                     /gene="sorE"
                     /locus_tag="POB34_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HDI5920879.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
BASE COUNT         2045 a         2009 c         2104 g         1994 t
ORIGIN      
        1 cacccaatca ggctgaaagc ggcgcaaata atccgccagc acgtcgggaa cttcgctgcc
       61 atcgagcgta atcagcaaga cttctggcgt atttaaccag ggtaaaagat gctcgcctgc
      121 atgtagcact ttgtagtaat cgccgtttct ggagagtaat gccagctgat cttttcttac
      181 gcttattttt ttaatcatta tctattcctt gcttaacagc cgtcgcggaa gcagacacga
      241 agcgacaggg gaattccctt ttgcctgtta cccggcatct ttatcggcga cgttatcggg
      301 gatcgctgac tgcgcaatcc ctcacttttt atttaataag ttaaaaccag tattcatttc
      361 aggaatgaat gtgggaatcg aacccataat tggcgtcaaa ggctgacgga acacgcccgg
      421 aaaacagcct ggccgttcct ggtgtgccga cggggtttga ccccctttgt aattcagttt
      481 caacgcgtac gagatattgc gagaagcgtg ccagaattga gaaaaaatta ttaaccaaat
      541 gaaaaataaa gatttaataa attatttttg tttgtagagg gaaaactttt cgctaaattt
      601 gtatctgtat ttatcgtcaa ttatcaagaa agataaatat ttaagaagag aacaaaggaa
      661 tattatcagt tcagctcgca aacgaattcc cgtacactga actctctggc tcactgaggg
      721 caccatcatg gcactccccc gtattaccca aaaagagatg actgaacgcg agcagcgtga
      781 acttaaaaca ctgctggatc gggcgcggat tgcgcatggt cgcgtactga ccaactccga
      841 aaccaacagc attaagaaag agtacatcga taaattaatg gtcgagcgtg aggctgaagc
      901 gaaaaaagcc cgccagttga agaaaaagca ggcttataaa ccggatcctg aagcgtcatt
      961 tagctggtca gcaaatacgt cgacgcgcgg caggcgctga ttagcgtccc ttctttttac
     1021 gccccggcga ggtaaaacgc ttaccgttgg acgccgggcg accgcctttt tccgccgttt
     1081 tttccatctt aacgactgga cgtttgatgc ccgctgtttt cggtttagcc ttcgctttag
     1141 gttttacctc ggaagaggaa ttttcaatga gtttaaacag gtcgattaac tcatcgtcag
     1201 ttaaatcgcg ccactcaccc agcggaatgc cgcttaaact gacgttcata atgcgcgtac
     1261 gttccagctt tttcacttca tagccgaaat gctcgcacat gcgccggatc tgacggttca
     1321 gcccctgcac cagggtaatg cggaagacaa acggcgcttc ttttttgact ttgcactttt
     1381 tcgtcaccgt cccgaggatc ggcacccccg cccccatacc acgaataaat tcatcggtaa
     1441 tcggtttatc gaccgtcacc agatactctt tctcatgatc gttacccgca cggaggatct
     1501 tattcaccag atcgccgtga ttggtgagga aaatcagccc ctgggagtct ttatccaggc
     1561 ggccaatcgg gaacacgcgt ttgctgtggt taacgaaatc gacaatgtta tcgcgctcgc
     1621 catcttcggt ggtgctgaca ataccgacgg gcttgttcag ggcgataagt accaaatctt
     1681 cggcttcccg aggctcaatc aactgaccgt ttactttcac aatgtcgccg ggtttcacct
     1741 gatcgcctat ggtggctcgc ttgccattaa ggaacacatt gccttgctcg atatagcgat
     1801 ccgcttcgcg gcgtgagcaa attccgcttt cgctgatgta tttatttaaa cggactgatg
     1861 agtcgggcag cataaattct cctgtaaaac gcggaatata ccgtacctct tagcggggaa
     1921 aaacagggtt atgcggcaac gtcgaaatta aatgtgatct gccacgcctt ttatcacaaa
     1981 agtgtctccg gaagtacgat gcaaatttgc ctggcaaaat aaggataatc aacatattac
     2041 gaatcgctgc acacctgtgc tgatgactac tacgcagaga aacaaatgtg caagagaaag
     2101 cgctatttct ccccatgcaa agagtgaaaa atcgctttga aaggccctca tgatggggtt
     2161 atatcagatc aagcggtgag aaagcgtatt tgcacaaatg tgcagaacca ggaggcggca
     2221 atggaaaaca gtgacgatat ccgtttgatt gtgaagattg cccaactcta ttacgaacag
     2281 gatatgacgc aggcgcaaat cgcgcgcgaa ctgcgtattt accgcaccac catcagccgc
     2341 ttgcttaaac gaggccgcga tcagggaatt gtcaccatcg ccatcaacta tgactacaac
     2401 gaaaatctct ggctggagca gcaactgaag caaaagtttg gcctgaaaga cgttgtggtg
     2461 gtgtcgggaa atgatgagga tgaagagact caactggcga tgatggggtt acacggcgcg
     2521 caactgctgg atcgcttgct ggagcctggc gatattgtcg gtttttcctg gggtcgcgcg
     2581 gtgagcgcac tggttgaaaa cttgccgcag gcggggcaat cgcggcagtt aatctgcgta
     2641 ccaattattg gcggaccgtc cggtaaactc gaaagccgct atcatgtaaa cacattaacc
     2701 tacagcgcgg cagcgaagct gaaaggggaa tcgcatctcg cggattttcc ggctctgctg
     2761 gataacccat taattcgtaa tgggatcatg cagtctcagc actttaaaac catctctgcc
     2821 tactgggata atctggatgt cgccctggtg ggaattggct caccggccat tcgcgacggc
     2881 gctaactggc atgcgtttta tggtggtgaa gagagtgacg acctgaatgc ccgccaggtt
     2941 gctggcgata tttgctcgcg cttttttgat attcacggcg caatggttga aacgaatatg
     3001 agcgaaaaaa cgctctctat cgaaatgaat aaattaaagc aggcgcgata ttccattggc
     3061 attgccatga gtgaagaaaa atacagcgga attattggtg cactgcgtgg aaaatatatt
     3121 aattgtctgg taacgaatag cagcacagct gaactattac tgaaataaaa atcttcccga
     3181 tgtttaccgt aataaaaatt acggcgggtc cctattatct aaaatcagga gtctgttatg
     3241 caaacgtggt taaatttgca ggataaaatc attatcgtca ccggcggcgc atccggtatt
     3301 ggtctggcga ttgtagagga attattagca caaggcgcaa acgtacagat ggtagatatt
     3361 cacggtggcg atggtcaata tgaaggtcat aaaggctatc agttttggcc gaccgatatt
     3421 tccagcgcca aagaggtaaa tcatacggta gcggaaatta tccagcgttt tggtcgcatc
     3481 gacggtctgg tcaataacgc cggggtcaat ttcccgcgtc tgctggtcga tgagaaagcg
     3541 cctgccgggc agtatgaact caacgaagct gcattcgaaa aaatggtcaa tatcaaccag
     3601 aaaggcgttt ttctgatgtc gcaggcggtg gcgcgacaga tggtcaaaca acatgatggc
     3661 gtgattgtga atgtttcctc agaaagtgga ctggaaggct cagaaggcca aagctgttac
     3721 gccgctacca aagccgcgct taatagcttc acgcgctcct ggtcgaaaga gctgggtaaa
     3781 cacggtatcc gtgtggtcgg tatcgcgccc gggattctgg aaaaaaccgg gctacgcacg
     3841 ccggaatatg aagaggcgct ggcgtggacg cgcaatatca ccgtcgagca gctgcgtgaa
     3901 ggctatacca aaaacgccat tcctattggg cgcgccggaa gattagcaga agtcgctgat
     3961 tttgtttgtt atctgttgtc taaacgcgcc agctacatca ccggagtaac cactaacatt
     4021 gcgggcggca aaacgcgcgg ctaaggaggc tttatggtca acgccatttt ttgtgcccac
     4081 ggcaaattag cttgcgccat gctggaatcg gttcagatgg tttacggcaa cgccaatgtt
     4141 gaagccgttg aatttgtgcc cggcgagaac gccggagaca tcgtagcaaa gctggaaaag
     4201 ttagtaagca ttcacaatca agatgagtgg ctgattgccg tggatttaca gtgcggcagc
     4261 ccgtggaatg cggcagcgat gcttgcgatg cgcaatcctc gtctgcgggt aatcagcggc
     4321 ctttctctgc cgctggcgct cgaactggtt gataaccagg acagcatgaa tgttgatgaa
     4381 ctgtgtgaac acctgacgca aatcgcaaag caaacctgcg ttgtctggaa acaactggca
     4441 acggcagagg aggatttctg atgaacatta cgctcgcccg cattgatgac cgcctgatcc
     4501 acggtcaggt cactaccgtc tggtcgaaag tggctaacgc ccagcgcatt attatctgca
     4561 atgacgaagt ttataacgat gaagttcgcc ggacattatt gcgtcaggct gcgccaccgg
     4621 gaatgaaggt taacgtggtc aatattgaaa aagccgtcgc tgtttatcac aacccgcaat
     4681 atcaggacga aaccgttttt tatcttttca ctcgcccaca ggatgcctta gcgatggttc
     4741 gccagggggt aaaaatagac accctgaata ttggcggcat ggcctggcgg cccggtaaaa
     4801 aacagttaac caaagcggtt tcattagatg atgatgatat taacgcattt catgagttaa
     4861 ataatcttgg cgtcatactg gatttacgcg tcgtagcgtc agatccctca atcaatatta
     4921 tcgacaaaat aaacgaacag ttaatcgcaa attaaataat agcgcctgtg tacatatgct
     4981 ttaacaggca gggatgacga cacttaaaag gtgaaaaatc atggaaataa gtaccctaca
     5041 aatcattgct atatttcttt tttcctgtat tgccggaatg ggcagcgtgc tggatgaatt
     5101 tcagactcat cgcccgttaa ttgcctgtac ggtgattggt ttaattctcg gtgatttaaa
     5161 aaccggaata atactcggtg gtacgctgga attgatagct ctcggctgga tgaacgtcgg
     5221 cgcggcgcaa tctccggatt ctgcactcgc cagcataatc tccgccattc tggttatcgt
     5281 tggtcagcaa agcatcgcca ccggaatcgc catcgcgttg cctgtggctg cggcaggcca
     5341 ggtgctgact gtgtttgccc gtaccatcac cgtagtgttc cagcacgcgg cggataaagc
     5401 agcggaagaa gcacggtttc gcactctcga tattctgcat gtctccgcgc ttggcgtgca
     5461 ggcgctgcgc gttgctattc cggcactgat tgtctcactg ttcgtcagcg ccgatatggt
     5521 gagcaatatg ctgagcgcga taccggaatt cgtgacgcgc ggtctgcaaa ttgctggcgg
     5581 ttttatcgtg gtggtcggtt acgccatggt gctacgtatg atgggcgtga aatatttgat
     5641 gcctttcttt ttcctcggtt tcctcgcagg tggctacctc gatctcagtc tgctggcgtt
     5701 cggtggcgtc ggcgtgatca tggccctggt ctacatccag ctaaatccac agtggcgtaa
     5761 agctgaacct caaccccagg ccaccacctc caccgccctt gaccaacttg acgattaacg
     5821 gagccatcat ggaacagaga aaaattacac gcagcgatct ggtgagcatg tttctgcgct
     5881 ccaacctgca acaggcgtcc tttaactttg aacgtattca cgggctgggc ttttgctacg
     5941 acatgatccc cgccatcaag cgactttacc cattaaaaga ggatcaggtt gcggcgctca
     6001 ggcgacacct ggtgttcttc aataccacgc cagccgtatg tggcccggtc atcggcgtca
     6061 ctgccgccat ggaagaggcg cgggccaacg gcgcggaaat tgatgacggt accattaacg
     6121 gcatcaaagt cggtctgatg ggaccattgg caggggttgg cgatccactg gtttggggaa
     6181 cgctgcgccc gattaccgct gcgctcggcg catctctggc actttctggc aacatcctcg
     6241 gcccgctttt gtttttcttt attttcaatg cggtgcgtct ggcgatgaag tggtatggcc
     6301 tacagctcgg ctttcgcaaa ggggtgaata tcgtcagcga tatgggcggg aatgtgctgc
     6361 aaaaactcac cgaaggcgcg tcgattctcg gactgtttgt gatgggcgtg ctggtcacca
     6421 aatggacgtc aatcaacgta ccgttggtgg tttcacaaac gcatgccgcc gatggctcca
     6481 ccgtcaccat gaccgtgcag aacattctcg accaactttg ccctggtttg ctggcgctcg
     6541 gtctgacgct gcttatggtt cgtctgctca acaaaaaaat taacccggta tggctgattt
     6601 tcgccctgtt tggcttaggg attatcggta atgcgctggg cttcctgtcc tgattcttcg
     6661 ccccggcacg actgccgggg ccatcgctca acatgaggtg gtttatgaaa acaacagctc
     6721 tgcgtcttta tggtaaacgt gatttacgcc tggaaacctt tgaccttcct gaaatgcagg
     6781 aggatgaaat cctcgcgacg gtggtcactg acagcctgtg cctctcttcc tggaaagagg
     6841 ccaatctggg tgaaaaccat aaaaaagtac ccgacgatgt ggcgaccaac cccatcatca
     6901 tcggccacga gttttgcggc gatattctgg ccgtgggtaa aaagtggcag cacaaattcc
     6961 agccgggtca gcgttatgtg attcaggcca acctgcaact ccccgaccgc ccggactgcc
     7021 ccggctactc cttcccgtgg gtgggcggcg aagccacgca tgtggttatt cccaacgagg
     7081 tcatggaaca agattgcctg ctggcatacg acggcgaaac ctattttgaa ggctcactgg
     7141 ttgaaccgct ttcctgcgtg attggcgcgt tcaacgccaa ctatcatctt caggaaggta
     7201 gttataacca cacgatgggg attcgcccgc aagggcgcat gctgatcctc ggcggcaccg
     7261 gaccaatggg actgttggcg attgattatg cgctacatgg acccgttaac ccgtcgctgc
     7321 tcgtcattac cgataccgac aacgataaat tgagttatgc gcgcaagcac tatccatcag
     7381 aaccgcaaac actgattcat tatctcaatg ccgccgatgc agcatttgat acgctaatgg
     7441 cgctgagtgg cggtcacggc ttcgatgata ttttcgtctt tgtgcctaat gaaggactgg
     7501 tgactctcgc ctcttccttg ctggcgacag atggttgcct gaatttcttc gccggaccgc
     7561 aggataaaca tttcagcgcg ccaattaatt tctacgatgt gcattatgca tttacccact
     7621 acgtgggcac gtcaggcggc aataccgacg acatgcgcgc ggcggtcaaa ctgattgaag
     7681 agaaaaaagt gcaggccgca aaagtggtaa cacatattct tgggctgaat gccgcgggcg
     7741 aaaccacgct tgaattgcct gccgtcggcg gcggcaaaaa gctggtgtat accgggaaat
     7801 acctgccgct gacgtcactc acgcagattc aggatcaagc actggcggcg attctggcgc
     7861 gtcatcaggg gatctggtcc ggtgaggcgg agcaatatct gctcactcat gcagaggcaa
     7921 tttcccatga ttaatcgcgc tacttaactc tgcatgtcgc tggctggtcg ccgcggcaac
     7981 ttcgcgacct gatgtgcggc tgattgctga agcggcccaa tgtgcacgag aaggataaaa
     8041 aaacatcagc gccttaaggg gcgctcaaat tggtggcaaa gtacacattg tttatgccgg
     8101 atgcggaata aagccttatt cggcctacat aatctcgaaa attcaatata tt
//
LOCUS       DAMZET010000179         8132 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.179, whole genome shotgun sequence.
ACCESSION   DAMZET010000179 DAMZET010000000
VERSION     DAMZET010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.179"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..370
                     /gene="yfdR"
                     /locus_tag="POB34_004022"
     CDS             <1..370
                     /gene="yfdR"
                     /locus_tag="POB34_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HDI5920880.1"
                     /translation="LVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKQMEEKIDAV
                     IREKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPG
                     HAYGMFMERFNELSELRKCA"
     gene            361..723
                     /locus_tag="POB34_004023"
     CDS             361..723
                     /locus_tag="POB34_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920881.1"
                     /translation="MRMNVFEMEGFLRGRCVPRDLKVNETNAEYLVRKFNALEAKCAA
                     LENKIIPVSAELPPANESVLLFDASGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPETKK"
     gene            779..937
                     /locus_tag="POB34_004024"
     CDS             779..937
                     /locus_tag="POB34_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920882.1"
                     /translation="MKLHSTETSWVAGRCESYDKKTGYRWGAPNMRRRLILDSIRPIK
                     QVATREQN"
     gene            999..1208
                     /locus_tag="POB34_004025"
     CDS             999..1208
                     /locus_tag="POB34_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004123007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920883.1"
                     /translation="MAKLMKASQWGRREFSNGSIPDNRTIKRWVENGLLMGRIVDGSV
                     FVCETEKWGVDSMVSQAVRKLINEG"
     gene            1211..2413
                     /locus_tag="POB34_004026"
     CDS             1211..2413
                     /locus_tag="POB34_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HDI5920884.1"
                     /translation="MAARPRKKEYRHLPDYLIFDKDRGVYKFTLITGKKKNIGKDRAI
                     AIAIAREYNLRMRPELSPSVENLIRESGGVIGEAKPFAEHVDHIMTRIIEDERPSQST
                     LDDWKNDALRVKAFFVNVPACDIELEHVNTYINKYHASASANVQNRKVSFLKKLFSYA
                     VDESLMLDNPAIRKKMRRTDEKKRRRLSLEHFIAIRQAAAPWLRTAMDLALQTTHARL
                     EVSRIRYSIREPKDGICGCVWLEQPKNGIYGTLYIHRQKVQKKEASHVAIPIGEELKR
                     IIDDSRDNVASPFVVHRIPERQVKRSKEVSHPTQVAPDYLSRSFSAIRDRLRLFDNVP
                     MEERPTFHEIRALAAHLFDQQGIDPQGRMAHSDAKSTKIYTKNHIDWVCVPHAEILAK
                     PEITKNFR"
     gene            complement(2397..3248)
                     /locus_tag="POB34_004027"
     CDS             complement(2397..3248)
                     /locus_tag="POB34_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920885.1"
                     /translation="MIALLLIPILVSGYILVVANLYHYYQLHRYDGQLLYIKVAAHGT
                     MSVVFVILAAVVVKYFFPNFHPIGMLSDILNVKKIINGNKAETWLALISLSSIIFSLI
                     WVALVWLKNMFFGFFCEKKHHENIFHAKKLRVLRKTISNSTLDSMLLDALESSPKEPI
                     LITLSSRKVYVGIVNGIQEPTESEAPNSYISIFPIMSGYRDKDTLSITFTNSYPSEVL
                     VSSAITMDSQKRHKVKNMDIIFRADEISHLSWFDFELFEQVNDSMKHDTIDLRSETYS
                     GETSENF"
     gene            complement(3253..3453)
                     /locus_tag="POB34_004028"
     CDS             complement(3253..3453)
                     /locus_tag="POB34_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920886.1"
                     /translation="MANNKQRHVDDMTDYARIRVGSRGGAYIPSEEIANLPEVRDMQQ
                     RAAAIVGRAARVQTMQKAKEKV"
     gene            3664..3741
                     /locus_tag="POB34_004029"
     tRNA            3664..3741
                     /locus_tag="POB34_004029"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3699..3701,aa:Pro,seq:ggg)
     gene            complement(3843..6212)
                     /locus_tag="POB34_004030"
     CDS             complement(3843..6212)
                     /locus_tag="POB34_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDI5920887.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGG
                     LAIAVDQKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYELDDGSGLIVMEDTQAI
                     DTILDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGSITYSSKAISENMVINNGRANV
                     WAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGASFRTHGAVDTSKADVSL
                     ENSVWTIIADITTTNQNTLLNLANLAMSDANVIMMDEPVTRSSVTASAENFITLTTNT
                     LSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASPAAGDSLTLVTTGGGDA
                     AFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTTDVLNMAAAQPLVFDAE
                     LDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQTLTGLTLGIDSRFSREE
                     SSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNGAYVDGVVKVDRFANTI
                     HGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAFTGFTTDGQDYTLSNGM
                     RADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEYADSNQVKVNDDGKFNN
                     DVAGARGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQAGVVWTF"
     gene            6532..7179
                     /gene="narP"
                     /locus_tag="POB34_004031"
     CDS             6532..7179
                     /gene="narP"
                     /locus_tag="POB34_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HDI5920888.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(7214..>8132)
                     /gene="ccmH"
                     /locus_tag="POB34_004032"
     CDS             complement(7214..>8132)
                     /gene="ccmH"
                     /locus_tag="POB34_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HDI5920889.1"
                     /translation="CQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGN
                     FVTYDPPLTPLTVLLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGY
                     VVYLPGIVVALIVAGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMS
                     RLALGMRTQLQKNPGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYA
                     EALTRSSDPNDNRLGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLK
                     LLPANDTRRAVIERSIAQAMQHLSPQESK"
BASE COUNT         2276 a         1962 c         1791 g         2103 t
ORIGIN      
        1 gctggtgccg caggaatttg cttttgaagc gttaatgcat gatgcaacag aagcgtattg
       61 ccaggacatt cccgcaccac tgaaacgcct tcttcctgac tataaacaaa tggaagaaaa
      121 aatagacgcc gtaatccgtg agaaatacgg gttaccccca gttatgagta cgcccgtgaa
      181 atatgccgat ctcatcatgc tggcaaccga acgccgcgat ctcgggcttg atgatggctc
      241 tttctggcct gtactggaag gtatcccggc aacagagatg ttcaacgtga ttccactggc
      301 accgggccat gcctacggga tgtttatgga acgctttaac gagttatcgg agttacgcaa
      361 atgcgcatga atgttttcga aatggaaggg tttcttcgtg ggagatgtgt accgcgagat
      421 ctgaaagtga acgaaacaaa tgctgagtac ctggtacgta aattcaacgc gcttgaagct
      481 aaatgtgcgg cactggaaaa caaaataata ccagtgtcag ctgaactgcc accagcaaat
      541 gaaagtgttc tgttatttga tgctagtgga gaaggctggc tgattggctg gcgttctctc
      601 tggtacacat gggggcaaaa agaaaccgga gaatggcagt ggacatttca ggtcggggac
      661 cttgaaaacg tcaatatcac tcactgggca gtaatgccga aagcaccgga gactaagaaa
      721 tgagcgtgat aaaaactcat acaggaattg ttatctcacc cgagacggtg aaaagcggat
      781 gaaattacat tccactgaaa cgtcctgggt tgccggacgt tgtgaatcct acgacaaaaa
      841 gactggttac cgttggggtg cgccaaacat gcgtcgccgt cttatcctgg acagcatcag
      901 gccaataaaa caggtagcaa ccagagaaca aaattaatta tcaggactgg aatttgatat
      961 tactgcccga gtgcagcggg ctaagtggag aaacagccat ggcaaaacta atgaaagcga
     1021 gccaatgggg acgccgtgaa ttctctaatg gctcaattcc tgacaaccga acgataaaac
     1081 gttgggtcga gaacggtcta ctcatgggac gcatcgtgga cggttctgtt tttgtctgcg
     1141 aaacagaaaa atggggagtc gactcaatgg ttagtcaagc tgttcgcaag ttaataaacg
     1201 aggggtgacc atggcggcaa ggccacgtaa aaaagaatac agacatcttc ctgattatct
     1261 gatttttgat aaagatcgag gtgtttataa attcacactt attaccggaa agaagaaaaa
     1321 tataggtaag gatagggcaa tagctattgc aatcgccaga gagtacaacc tgaggatgag
     1381 accagaatta tctccatcag tagaaaatct tattagagaa tctggcggtg ttatcggaga
     1441 agcaaaacca tttgctgaac atgttgatca cataatgacc cggattatcg aagacgaacg
     1501 tccttcccag agtaccttag atgactggaa aaatgacgct ctacgcgtaa aagcgttttt
     1561 cgtaaatgtt ccggcatgcg atatcgaact tgaacacgtt aatacctaca tcaacaaata
     1621 tcatgccagc gcatccgcaa atgtgcagaa cagaaaggtc agctttctta aaaagttgtt
     1681 ctcttatgcg gtcgatgaat cactaatgct ggataacccg gccatcagaa aaaaaatgcg
     1741 taggactgat gagaagaaaa gacggcgcct gtcactcgag cattttatag ctatcaggca
     1801 ggccgctgca ccatggttaa gaacagcaat ggatctagca ttgcaaacta cacatgcacg
     1861 actcgaagta tcgcgaatcc gttactcaat tcgcgaacct aaagacggca tctgcgggtg
     1921 cgtatggtta gaacagccaa aaaacggtat ttatgggaca ctatatatcc ataggcagaa
     1981 agtacaaaaa aaagaggcat cacacgttgc aataccgatt ggtgaagagt tgaaacggat
     2041 aattgacgat agcagagata atgtggcaag tccgtttgtc gttcacagga tcccagaacg
     2101 ccaagttaaa cgaagcaaag aggtttcaca tccgacacaa gttgccccag attatttaag
     2161 tagatcgttt tccgcaattc gtgatcggtt gagactattt gacaatgtac ccatggaaga
     2221 aagaccaacc ttccacgaaa tccgtgcgct ggcggcacac cttttcgatc agcagggaat
     2281 agatccgcaa ggccgcatgg cacacagcga cgccaaatct acaaaaattt atactaaaaa
     2341 tcatatcgat tgggtgtgtg tacctcatgc tgaaatttta gcgaaacctg aaattactaa
     2401 aaattttcgc tagtttcccc tgagtaagtc tcacttcgca agtcaatggt atcgtgcttc
     2461 atcgaatcat tgacttgttc aaatagttca aaatcgaacc atgaaagatg agaaatttca
     2521 tcagcacgaa atattatatc catatttttc actttatgcc ttttttggga gtccattgta
     2581 atagctgaac tcactagtac ttctgatgga tatgaattcg taaaggtgat ggaaagagta
     2641 tctttatctc tatagcctga cataataggg aaaattgata tataactatt tggcgcttcg
     2701 ctttctgttg gttcttgaat tccattaact ataccaacat aaactttccg tgaagataag
     2761 gttattagaa tcggttcttt aggactgctt tctaatgcat ctaaaagcat actatccaaa
     2821 gttgagttag aaattgtttt tcttaatact ctaagtttct tagcatgaaa gatgttttca
     2881 tgatgtttct tttcacaaaa gaaaccaaaa aacatatttt ttaaccaaac taaagccacc
     2941 caaattaaag aaaatataat tgatgataat gaaatcaaag caagccatgt ttcagcttta
     3001 ttaccattta ttattttctt gacattaaga atatctgaaa gcattcctat tgggtgaaaa
     3061 ttaggaaaga aatatttcac tactactgct gctaaaatca caaaaacaac actcattgtt
     3121 ccatgagctg caacttttat atacaaaagt tggccatcgt atcgatgcaa ttgatagtaa
     3181 tgatataaat tagcgactac taaaatgtag ccgctaacaa gaatgggaat cagtagtaaa
     3241 gcaatcacct agctaaacct tttcctttgc tttttgcata gtttgcactc tagcagctcg
     3301 tcctactata gctgctgctc gctgctgcat atccctgact tcagggagat tggcaatttc
     3361 ctcagagggt atgtaagcac ctcccctcga gccaactctg atgcgagcat agtccgtcat
     3421 gtcatcaaca tgtctttgtt tgttatttgc catttgctcc tcctcatcaa gctgaggttt
     3481 caactcaatc ttatgtcaag gcagaaaccg tagcaaactt ttatcaaaaa aaaggcttgt
     3541 gatcaaaaag taaaaaaatt atatggggag aaaccaccac ctaagtcatt gatgtatata
     3601 gcgtgaattt tgcgaattat acactgtttg cgcatacagt aaaacacccc accagcccag
     3661 taacggcgcg gcctccagcg tttcaggcgc ggtgacatgg ggtgtcgggg gtcggaggtt
     3721 caaatcctct cgtgccgacc aaaaatccca aaaaaaacca accattacgg ttggtttttt
     3781 tatatctgca attaattcga taaacagacc gtgacacatc acagcctgtt tattttctgt
     3841 tatcagaacg tccagaccac acccgcctga gtattccacg gcgattctac ccctgcgcca
     3901 ttgccatagc tgactgacaa atgaccgctt aacgtcgggg taaacgatga ccttatccca
     3961 gcctgataaa cgccacgggc tccagccaca tcattattaa atttgccatc gtcattaact
     4021 ttcacctggt tagaatcggc gtattcctga cgtacggcgg ctttcagcca gggttccagc
     4081 gtcgtaccat tttgcaggtc catgtgatag cttaccgccg ttcccgcttc agcgcgtaat
     4141 atccgggtat ttcccacatc agcgcgcatg ccgtttgata acgtgtagtc ctgaccatct
     4201 gtggtaaagc cggtaaaggc cagatagggt ctaacactcc acaatccgtc aacccaacgg
     4261 aacccgctct caacatgagc acccgcgccg ttactattgt aatcgccaaa cgctgttgcc
     4321 ccattactca tcttgccatg gatggtgttg gcaaaacggt caactttcac caccccatca
     4381 acataggcac cgttctgatg ctcccaaccg gcataagccc ccagggtata gctatcgata
     4441 ttaccttttc cgccgcgatc aaaaccaata tcagaatgag agtaaccaaa gaacaagccg
     4501 cgaattgtac tgctttcttc acgggagaaa cggctatcga taccgagcgt caggcccgtc
     4561 aatgtttgct caaaaccagc tcccgcatca gtggtcacgt tgttgcgggt gttaattgcc
     4621 gaactccaca tcgccgtatc gtaactaacg ccttttacgc taccaagacg ctcacgcacg
     4681 gtgtccagtt ctgcatcaaa taccagcggt tgtgcggccg ccatattcag cacatcagtg
     4741 gttgaagggg taatttgcgc gcgattctct gccaaactcc agctatggtt gccattatcc
     4801 agcaaggtat attcatacgt accgatatca acaacgcctc cagcattgcc caacgtaaat
     4861 gcagcatcac cgccgcccgt tgttaccagt gtaaggctat ctcctgctgc cgggctggca
     4921 ccggtgtccg tcacgaatat tttgaaatca cctgttgcct gaccggtgac gttgagctga
     4981 tcgctctgat gattagccat atcggtacgc atataaaaat tgccgtttcc cgacagggta
     5041 ttggtggtca acgtaatgaa attttccgca cttgccgtca ctgatgaacg agtcactggc
     5101 tcatccatca taatcacatt tgcgtcagac atcgcaaggt tggctaagtt gaggagggtg
     5161 ttttggttcg tcgtagtgat atcggcaatg atggtccata cgctattttc gagcgaaacg
     5221 tccgctttgc tggtatccac ggcaccatgc gttctaaaag aagcgccctc agaaaccact
     5281 accttaccca ccaacattgc gggtgcataa tttatttgcg gcttcatgac ctcaagaatg
     5341 ccatcattcc ctctgactga aacgttaacc attgtgccag cccagacgtt agcgcggcca
     5401 ttgttgataa ccatattttc agagatggct tttgaggaat acgtgatact tccattctga
     5461 tctgatcgac cgagatcata caccgcattt gcctgcactc tcgtcccagt atcctttccc
     5521 agcgattgca tagtggcatg ctcatcaagg atagtatcaa tggcctgcgt gtcctccata
     5581 acaatgagcc cggaaccatc atccagttca taattttttg acacaccatc ttttatacta
     5641 aattgacctt tactgtttgt accactcact tccagcgcat tcgttgagac aattaatttt
     5701 ccgcctgctt tctgatctac cgcaatcgct aatcccccct ctttaacctc cagtaatcct
     5761 cccttttcga tagcggcaag gacggtaatc ccatcttttt caacgattaa gcgcccgcct
     5821 ttaatcgtgg gatcattcgc tgcgccataa acctgtagca caccattatt gagggtagtc
     5881 tggtttgccg cgccacccat ttgaacaatt tgataaccgt catttaatac tgagtattct
     5941 gcggtgccat tcatttcaat gtactgatac ccgccgttaa tttcagtatt actacttata
     6001 ccaaattctt taatatgctg ttctccgcca ggattgattt tcgtattatt ggcggtccca
     6061 taaaccagct ggatatcttt ttccaggaca acaccatcaa ctgtctcacc atactcaact
     6121 gttgatgcca atgctatggg tgaatataac gctgtagcca ccagaacaga tattgcggaa
     6181 cgacaaagag aaacagaacc agattgatgc attgagcttt catcctatga aattaattgc
     6241 agttaaaagc attgggtaca gaaaataccc atagctccat acccggagtc agtttttaaa
     6301 aactgtttaa agaaatgcac aagtattgtg attgattttt tagttgtttt tcttgatgag
     6361 aagctgatgc aaaattccgt ctttataatg aaaatgatgc caaagcgaac gacaaggttg
     6421 tagttttcac tacatgtcca tacataaaat ggggtaacat tcacgcgcct ggtagcgtta
     6481 ccaacgctac gctcaaacat aatgattcta ataaaacctc aggagactac tatgcctgaa
     6541 gcaacgcctt ttcaggtgat gattgtggat gatcatccac ttatgcgacg cggtgttcgt
     6601 cagttactgg agcttgatcc tggctttgaa gtggtcgccg aagcgggcga cggcgcgagc
     6661 gcgatcgatc tggcgaatag actggatatc gacgtgatct tgctggatct caatatgaaa
     6721 ggtatgagtg gcctggatac cctcaatgcc ttgcgcaggg atggcgttac cgcgcaaatt
     6781 attatcctga ccgtatccga tgcctccagc gatgtctttg cgctgataga cgcaggcgca
     6841 gacggttatc tgttgaaaga cagcgacccg gaagtattgc tggaagcgat tcgtgccgga
     6901 gcgaaaggca gcaaagtctt tagcgaacgc gtcaatcagt acttacgtga acgtgaaatg
     6961 tttggcgcgg aagaagatcc cttcagcgtg ctgacggagc gcgagctgga tgttctgcac
     7021 gagctggcac aggggctgtc aaataaacag attgcctcgg tgttgaatat ttccgagcag
     7081 acagtaaaag tacatattcg caatctgctg cgtaaactca atgtccgctc acgcgtggcg
     7141 gccaccattc tgttcctgca acaacgcggg gcacaataaa aatagcccga tggcatcacc
     7201 atcgggcctc tttttattta ctctcctgcg gcgacaaatg ttgcatcgcc tgcgcgatac
     7261 tacgttcaat caccgcacgg cgagtatcgt tggcaggtaa gagtttcaac atcatctccc
     7321 acgcggcaac ggcttcgcca aatcgctgct gctcaaaggc gttaaacgca tacatgctta
     7381 acacacgaat attgctatgg tccgttctca ccaactggcg cagcagttcg ccgccgagac
     7441 ggttgtcgtt gggatcagat gaacgtgtca acgcttcagc gtatcccagt gcagcatcgc
     7501 tgttcttcgg atcgaggcga tacgcagtgg cgtatgcatc ggtggcgata ctggcgttac
     7561 ccagcgccat gccaacgcgg cccaacataa tccagccttc tatatcaccc ggattttttt
     7621 gcagttgagt acgcatcccc agcgcaaggc gcgacatctc ttcttcgttg agcggatcgg
     7681 ctttcggatc cagcgccctg tccagcaacg ccggagcctg tgccgtggcc tgctgccaga
     7741 ttttcacctg ctgataattg ccagtctggt agtagctgac gccagccaca attagcgcca
     7801 ccacaatacc cggcagataa acaacatatc cggcacgctt accttccggc acgctttgtt
     7861 caggaaacgc ttccggcacc acgcgtaccc gacgccgcga acgggcgtaa atgacccaac
     7921 cgccaatgcc aatagccact accggaagca cccacagcag cacggtcagc ggcgttaacg
     7981 gcggatcgta agtgacgaag ttgccgtaac gcgccaccat ataatcgaca atctcttttt
     8041 tacttttacc ttcctgcatc agttcataca ctttctggcg caggtcggtg gcaatcatcg
     8101 agttggaatc ggcaatgctg ttgttctggc at
//
LOCUS       DAMZET010000180         8111 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.180, whole genome shotgun sequence.
ACCESSION   DAMZET010000180 DAMZET010000000
VERSION     DAMZET010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.180"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..778)
                     /gene="pgaB"
                     /locus_tag="POB34_004033"
     CDS             complement(<1..778)
                     /gene="pgaB"
                     /locus_tag="POB34_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HDI5920890.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFVWPYGEANGIA"
     gene            complement(787..3210)
                     /gene="pgaA"
                     /locus_tag="POB34_004034"
     CDS             complement(787..3210)
                     /gene="pgaA"
                     /locus_tag="POB34_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HDI5920891.1"
                     /translation="MYSSSRKRCPKTKWALKILTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALLWFAQKSALSNNQIADWLQIALWAGQDKQVITVYNRYRYQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            3797..5155
                     /locus_tag="POB34_004035"
     CDS             3797..5155
                     /locus_tag="POB34_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDI5920892.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCVLSLAFLSCLIYFVKTVIIIQQIIEER
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFG
                     GAIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRY
                     DIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKNIAHRDPLTNIFNRNYFFNELTVQSASAQKTPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQENALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCE
                     SP"
     gene            complement(5196..6358)
                     /locus_tag="POB34_004036"
     CDS             complement(join(5196..6097,6097..6358))
                     /locus_tag="POB34_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HDI5920893.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QLYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKY
                     VFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRTSTRQQFRQHCDSVVLAAFTRSK
                     QRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLL
                     EQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDAL
                     QMALWRRKRPRNIIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
     gene            6425..6952
                     /locus_tag="POB34_004037"
                     /pseudo
     CDS             6425..6952
                     /locus_tag="POB34_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
     gene            6949..7935
                     /locus_tag="POB34_004038"
     CDS             6949..7935
                     /locus_tag="POB34_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920894.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRCLWHNDFMMACDMYFRWGHFNK"
BASE COUNT         2066 a         1775 c         1881 g         2389 t
ORIGIN      
        1 tcgctatgcc attcgcttcg ccataaggcc aaacaaaaac gtgtggattt acctcaacct
       61 ttgtacgcag gtattccgtc atttttacag catccagacg aattctttcc cggtattctg
      121 ctgcggtttc ataccgtgcg tggtcagtaa aatatgcacg atttacatat acaggcaata
      181 agctgccggt ggcattagcc tgaataccgt agtgagaatt ccatgtatga gaagcgagct
      241 caacgagccg ggaacgcgca acttctcgca cttgttgcca cgtggcaaaa tattctcgat
      301 cgaccaactc atcgccaaat tttacttgtt tatccgctgg cgtatcgacc caactgccga
      361 cgggggccca tacagcaggc cactggaagg cctgaagaat tgggaagacg cgggtataaa
      421 aactctggta gccgtcatca aaagtcagca ctacagcttt ttccggtagc ggttttcctc
      481 ctcgatgtgc ttcacgaatt tgagcaatac tgaccggttg ataaccgttc tcgcgcagcc
      541 aggcaaattg ttcacgcagt gctgatgtcc gcactgacat aaaacgctgg tcggcagctt
      601 cgtcttcaac gttatgccat gaaatcgcta caaaaccatt atgcggccac ggttgctcgg
      661 cgagtaaaga ttcgcgatcc tgtggcggta taaatgatgt tcttgactgg ctaatgcacg
      721 cggtgagcat aattatactc accagcatca ggagatattt atttccatta cgtaacatat
      781 ttatccttaa aatctgaatg tcatatcgaa ttcaacgtat aagttgtgtt ctctgtcacc
      841 gtcataaggt cgtttttccc agcgtagcgt tgcgccagca tcaatcacgt cattccaact
      901 aatgcgttgc ccgtagccga gttgggtgac gacatccgtg ccataatgtt tttgccagga
      961 ggcaccaaca cctgcgctga atatttgctc ccagctattt tcatagcttc gccataacaa
     1021 atggcttgcc tcaaatgccg gaacaatatc gaacgttttt atagggttgt agtatggggt
     1081 atcgtgttct gtattttgtt cgtaatacag actgggtagg aaatcgacaa tcaaatatgg
     1141 tgaagaccag atgcgttcct gaccctcaag tgagacttca tgacgctggt tactgtcgga
     1201 aaaatcagtg aaagcccagg agacaccgta cttacgccgc tcattttgat accagcgaac
     1261 ataagcctga gcactgttgc ctgtaacacc atttttcatt gcccgtaatg gaacgcggtg
     1321 agagaggcgt tccagttgcg aaccaatacg ccagttatca ttaaaatcat accagccaga
     1381 caggcgcgcg ccgggtttat gctcatgatt gaaaacgcgt tcagcgtact ctgcctcgag
     1441 ccagatatta cgtgaccgcc actcaacacc cgcaagccag tcgcgaacaa tcccttttcc
     1501 ttcgctaaat tgtccatcgg cataaccgaa tccagcaaaa ccgcgccagt tatccttcag
     1561 cggtggtgaa taaacgatgg tggttaagtc gacatcatgt ttaccactat ccgggccttc
     1621 ggcatcaatt cctgttgaac cagctatacg aagctctgca agattatgta catcaaccgc
     1681 acgttttaaa cgtacaacgc cgggatcttg cggttcacgt tcgacaacat cgtgcgttaa
     1741 gacagctgcc tgctgccatt cttgtaacgt taatgctgtc caggcttgtt caacctccag
     1801 attaatatta cgtggctcga tcacttctgc tttttttaat tcattttctg ctgcacgagg
     1861 ccaaccgcgg gcttgtaaca cactcgcgta atcaatgcgc agtccctgat ttccaggtgc
     1921 gttataagca agctctctgg ctgtcatttc agcctgagga agatcattac tatattttgc
     1981 tacggttgag agaaacgaat gcccctgtaa ccaggtatca ttcgggatgc tcgtaggcgt
     2041 gcccattaac cgaaggaaag gcggcgaagt attaatggta tgttgggtga cagttagcgc
     2101 gcccggataa ttttcactct ccaggtggct gtaaaagaga tccgcaagtt cttcatcgga
     2161 taaatccggg gcaatggtct ccttgtgata aaagagctcg gtcattattg actgtgcttt
     2221 tttcggctga tgatctttga gataagccga tgcaacccaa tattgccccc agggcggaat
     2281 aatttgcccc gtctttttta atcgctgata gtgagaaata acgtctttat aacgatcgcg
     2341 agttaataac gcgccaagat gatcaacctg aatacgctgg tactgggcag tgcggtctgg
     2401 gttatcgtgc cacagaattt ctaatgcagc gtattgggcg agggcgcgat cggcaatggc
     2461 ataacgttca ctttcactgc gcgtaggcat aaacgacagt ctgaccagtt cggcatgaat
     2521 atcagcgcga atatctggcg ttaaattggc atcgtcaatc gcggcagcaa gttgattatt
     2581 acgtaatgcc tgcacgtatt ctgtgggata ttgttgcgta gatgcatttt caggtaatga
     2641 ctctgtcatc gcccgtaatt catcctgatg acgccccgcc agtttataga tataggcttc
     2701 tgcgagtaaa ttggctttgt ccggtgctcc agagttaagc tgcttaagtt taaccagcgc
     2761 agtatcatag tgacccgcat ctgccagggt taaaatttgt ccccgttgat aatccttatt
     2821 ttgcggctcc agagagagcg ccttttgcca cagtgtaagc gagttttgcc attgttgcag
     2881 gttacgataa gcgacggcga cagctgcata accacgcgct ggtaattgct gataacggta
     2941 gcggttgtaa acggtaataa cctgtttatc ttgcccggcc cataatgcaa tctgtaacca
     3001 gtcagcaatt tggttattac tgagtgctga tttttgtgca aaccataaca aagctggctg
     3061 agtatttccc ttgcgagctt caataatcaa tgcatcatag gcgttattaa cggcactctt
     3121 cgccgcggga ctcgctgcta aaaatgcggc agtaagaatt ttcaaagccc atttggtttt
     3181 cgggcacctt tttctgctac ttgaatacat cctgtattac tccatgtatc gccaaaatct
     3241 ctctctgtat ctaattacag gtaactgaaa agaaagatat ttttgcacct cataatccgt
     3301 tattaaacgc ggaagagaga cgtgaattgt tgatgatgag aagaagaaat gatgagcaga
     3361 gtgtccatat aaaatccttt tctcgcccga aaatccattc caatgatgag gatcttcagg
     3421 aatacggcat aaatcccaat gcctttttca aaataaatta ggattaaaat aattaaatca
     3481 gtaaattccg atgcatgatt tcggattttt cgaaagtcct gatgaaaggc tgcgctacgg
     3541 cactcacgga tttaattgtt attcctaatg tatctatgaa ttaatgtttt ataaggattt
     3601 tctataacca tttataagta ttttcaagcc tggcttgttg caaacaatgt ataaagcact
     3661 taggcaataa taattacatt cagcaactat catcatcggt attgtttgtg ggcggaattt
     3721 catattgaca acagtacagt tcttatatct attaataata gaaagggatc tacaacctac
     3781 agattggtgt agctttatgg aaaaagacta tttgagaatt agtagtactg tattagtgag
     3841 cttattgttt gggcttgctt tggttttggt gaatagttgg tttaatcagc caggcgttga
     3901 ggaagttgtt ccacgatcaa cctatctgat ggtaatgatt gctttatttt ttatcgatac
     3961 tgttgcattt atttttatgc agttgtattt catttatgac cgtaggcaat tttcaaactg
     4021 tgtacttagt ttggcttttc tgagttgttt gatttacttt gttaaaaccg tcattatcat
     4081 tcagcaaatt attgaggagc gtttgacaag cagtgttgtc caaaatgaca ttgcaatcta
     4141 ttatttgttt cgtcagatga gtttgtgcat attaatattt ctggcattgg tgaataaagt
     4201 tagtgaaaac acaaaacagc gcaatttatt ttccaaaaaa atgactttgt gcataagttt
     4261 gttttttgtt tttggggggg cgatagttgc tcacatactt tctagtcatt atgagagcta
     4321 caatttacac attgccgagt taaccaatga aaatggtcag gtggtttgga aagcctcata
     4381 tgttaccata atgattttca tgtggttaac cttgctttcg gttaatctat actttaatgg
     4441 tttacgctat gatatctgga atggagtgac ggtgattgct ttttgtgccg tactgtacaa
     4501 tatctctctg ttatttatga gcaggtatag cgtgtccact tggtatatta gtcgcactat
     4561 tgaagtagtc agtaagttaa ccgttatggt aatatttatg tgccatatat tcagtgcgct
     4621 acgagtaaca aagaacattg cacatcgcga tcccttaacc aatatattta acagaaatta
     4681 tttttttaat gaactgacag ttcaatcagc atcagcccaa aaaacgcctt attgcgtcat
     4741 gattatggat atcgaccact tcaaaaaagt caacgacacc tgggggcatc cggttggcga
     4801 tcaggtgata aaaacagtgg tgaatatcat tgggaaaagc atacgaccag atgacctttt
     4861 agcgcgcgtc ggcggcgaag agtttggcgt cttgctgacg gacatcgata ctgaacgtgc
     4921 gaaagcttta gcggaaagga ttcgggaaaa tgttgagcgt ttaactggcg ataatcctga
     4981 atacgctata ccacaaaaag tgacgattag tattggcgct gttgtgactc aggaaaatgc
     5041 gttaaaccca aatgagatct atcgactggc tgataacgca ctttacgagg cgaaagagac
     5101 cgggcgtaat aaggtggttg tgagggatgt ggtgaatttt tgtgagtcac cataaagcgg
     5161 cattttgatc ctacccacgt aatatggaca caggcctaag cgaggttctt gttttcaaat
     5221 tgttccggac tgaggccgcc acaccaactg tgccgccgcc accgattgta atcacattcg
     5281 atataattaa acaccgttgc ccgcattatt tcccggctga taaagtgttc tccatggata
     5341 cattccactt tcagcgaatg aaagaagctt tccacgcagg cattatcgta gcagcaacct
     5401 tttgcgctca tacttccacg cagattatgc cgcttcagtt gcgcctgata atctgctgaa
     5461 cagtactggc ctccacggtc cgtgtgaacg ataatgttcc ggggcctctt acgccgccac
     5521 agcgccatct gcagggcatc gcaggccagt tgcgccgtca tgcgtggcga cattgaccag
     5581 ccaataacgg cacgtgacca caggtcaatg accactgcca gatacagcca gccttcatct
     5641 gtacgtaagt acgtgatgtc tcctgcccac ttctggttcg ggccactggc gtaaaaatcc
     5701 tgctccaaca gattttctga cacaggcagg ccgtgtgcgc ggtagctgac cgggctgaac
     5761 ttccgggagg cctttgccct cagtccctga cggcgcaggc ttgccgccac ggtttttacg
     5821 ttaaaggggt aaccctgagc acgcagttca tccgtcaggc gtggggcacc gtaacgctgt
     5881 tttgaccggg taaaagccgc gaggacaacg ctgtcgcagt gttggcggaa ctgctgacgc
     5941 gtgcttgtcc ttgtccgccg ctgacaccac gtataccagc cgctgcgggc cacccggagc
     6001 acgcggcaca ttgctttgat gctgaactca gcctgatgtt tttcaataaa gacatacttc
     6061 atttcaggcg cttcgcgaag tatgtcgcgg ccttttggag gatagccagc tcttcatccc
     6121 gttctgccag ctggcgtttg agacgtgcaa tctcggtaga catctccagt tcacgttcag
     6181 aagacgtctg ctgattttgc tgtttactgc gccagttgta gagttgtgat tcatacaggc
     6241 tgagttcacg ggctgcggca gtaacaccga tgcgttcagc aagcttcagg gcttcactgc
     6301 gaaattcagg cgaatgctgt ttacggggtt ttttactggt tgatactgtt tttgtcatgt
     6361 gagtcacctc tgactgagag tttactcact tagccgcgtg tccactattg ctgggtaaga
     6421 tcaggcggtt aaaggcatca accgcgaaca actgcagacg atgattacgg aatcaggcat
     6481 tgcgctggat attagctgtg ataacacgcc gcgccagcag gttattgggg gaacgcaggc
     6541 ctcgttgaat gaatttgcca cgctgctgat ggccgctgga tacgaaccgg ttaaactcgg
     6601 tgtgagcggt gcctggcaca cgcgtttgat ggaagacggc gttcaggcaa tgcgcgatta
     6661 tcttgccgga ctggatatcg cttcgccaga gcatcaggtt ctgatgaacg taacagcaaa
     6721 aagcgaggtc gctccgtcaa ttatcaaaga aaatctctca ttacacttaa cccatacggt
     6781 gaaatggact gaatctctcg atacatttct gaatatgcca accccggtag ctttccttga
     6841 aataagtaat aagccctatt tggggaatat gttaaatgat tttgttggcg tggatcagca
     6901 acgagttatg cattgtcgta aagcatttag tgatgcaaag gtattcaaat gattcctgat
     6961 tatttaactt ttattcgctt tcaggataaa cgaaatctga tatacattta tgctattgga
     7021 cttattctga taggctttta ttggaaaaat gcaggattta cttttccatc agaggatatt
     7081 ggtgtcgtta gtgggattct ggccctggtg ctgtataatt ttatttttga tctcaaggcg
     7141 tactgggctt ataaatgcgt cacgaagaat atcgattttt cgtggtttaa gaaaaagcag
     7201 aaccacaaaa tagaattatt tcttacacaa cctctggtgg caggatttct gtcgttaatc
     7261 atgttgagtg caatgagttg ggggctatac cagcttctac cctcgttata tgcgctgttc
     7321 ctgatttcgt tacttgggcc gttggtcatc tttctgctgt ttcggatgat ccgcaccagt
     7381 tatgtcaagc aggtcgctat ttcagtagcg aaaaaagtaa aatataaaag tctgactcgc
     7441 tatgtgctgc tttcggtgtg catctcaacg gttgttaacc tgcttactat cagcccgtta
     7501 cgtaacagtg attcttttgt gacagagggg cagtggttaa cgtttaaatc gataattgca
     7561 ttgctcattc tttgtggcgt agtgttggcg attaatctgt tttttctgcg cttctccaag
     7621 cggtacgctt ttctgggcag gctttttttg caggaaatcg atctgttttt ctccagtgaa
     7681 aatgcgttgt cgaccttttt tgccaagccg ctttggcttc ggttattcat attgctggtt
     7741 attgaaatga tgtggattac gctggtgtcg gtattggcaa cgcttgtgga atggcggatt
     7801 tggtttgaag cctatttttt actctgctat gtaccgtgct taatttacta ttttttccat
     7861 tgtcgatgcc tctggcataa cgattttatg atggcatgtg acatgtattt ccgttggggg
     7921 cattttaata agtgaggaag ttacaggaag tgaccagata acgcatatat gttctctact
     7981 atcttgcgca ttttgattgt tgactgagta accagacagt tgatgtgcac aattttccct
     8041 cgccctaaca gacgtgggcg ggggcacccg gtgtgtgcaa tatacgaaaa aaaagcccgt
     8101 actttcgtac g
//
LOCUS       DAMZET010000181         8070 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.181, whole genome shotgun sequence.
ACCESSION   DAMZET010000181 DAMZET010000000
VERSION     DAMZET010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.181"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(315..1016)
                     /locus_tag="POB34_004039"
     CDS             complement(315..1016)
                     /locus_tag="POB34_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920895.1"
                     /translation="MKIALVFRSGGDYNASDVQWLVNQLPKGYEIICLTDLKRLHVPG
                     VKVVPLINQWQKCRGWWAKIELFRPDITDDLFYLDLDTVIAGDIRPILENPPTSFTML
                     RDFYHPQYRGSGALWIPNSVKAHIWSAFWQDPEGWIARCVTTECWGDQGFLRKVMGDD
                     TPAFQDLYPGWFVSYKADVVEPGSKYASARYSRGNGALPKDCRIIFFHGKPRPREVSE
                     DWLPFISAFFEQEPE"
     gene            complement(1013..1690)
                     /locus_tag="POB34_004040"
     CDS             complement(1013..1690)
                     /locus_tag="POB34_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDI5920896.1"
                     /translation="MMAPTIYHRIDGTKYRNVWVVGDLHGCYTRLMSELHRVDFDPAQ
                     DLLISVGDLIDRGTENVECLELLQMPWFRAVMGNHERLMIDALSPDGNVNNWLMNGGQ
                     WFFMLDTDQEILAWALVELVKRLPYIIELNTGQETIVIAHADYPDNEYQFGKEVPLFN
                     VVWARERISDSMDDIGGEISGADRFIFGHTPVKSPKTFWNQQYIDTGAVFCGNLTLMK
                     VKGDGAA"
     gene            complement(1687..2313)
                     /locus_tag="POB34_004041"
     CDS             complement(1687..2313)
                     /locus_tag="POB34_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="norphogenetic protein"
                     /protein_id="HDI5920897.1"
                     /translation="MFPKNKFRGSDRVIIVGSGPSAANFVAPRGVPIIAVNGAIDWLN
                     RASYFFTLDPSPDNMRRVGRGRRRRGVCYCMALPDVKEREVRDGVLCFRRVAERGMEP
                     KNTNSPEWWAWRWSAHFGLCEDENEIASGNSAYGALNLAFHIGFKHVALVGVDATQEP
                     RVHSGGTPKNLSHLPLLFQSAREQIDVVSCGKMGGIPQMTLKEWLKNT"
     gene            complement(2815..2970)
                     /locus_tag="POB34_004042"
     CDS             complement(2815..2970)
                     /locus_tag="POB34_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="norphogenetic protein"
                     /protein_id="HDI5920898.1"
                     /translation="MSSKVNYESLASVMPRNEQETDAVVDPVIAEMNARLEAEFAAEN
                     EHTTQGD"
     gene            complement(3037..3615)
                     /locus_tag="POB34_004043"
     CDS             complement(3037..3615)
                     /locus_tag="POB34_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HDI5920899.1"
                     /translation="MLRFTEEEFQAFSERRNKGRSRPKTKKDPFLSLAPVKEVSPHAK
                     ALAALAKNPDLRDGNCEHFEQVFIFDYFERKHPDIYELLHATPNGGKRSKATAGKMKA
                     EGQKKGYPDMSLDKACGIYHGMRIELKEPNGKAPTKEQIAWMRRLREEGYYVVLAYGA
                     EQAITAILEYISLKKGEAIEHVLNGDKWLYAA"
     gene            complement(3618..3863)
                     /locus_tag="POB34_004044"
     CDS             complement(3618..3863)
                     /locus_tag="POB34_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920900.1"
                     /translation="MAERVDDAELSMNQLEALKDMAIDNIRKQAQVVSQVFTGKCRYC
                     NESIESGIYCDAECAQWHREEQAAKQRKYGMRPAGFD"
     gene            4010..4387
                     /locus_tag="POB34_004045"
     CDS             4010..4387
                     /locus_tag="POB34_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920901.1"
                     /translation="MATSITTTQSTRQYPLSRYDDRNIADPILRAELRKEVMLMCESN
                     DKNLTIYYVLPDEQYRPDLLAYRMWGIAELRWVVTLAAGLEDESQGMTVGKKLKLPPA
                     TWIREMIRHFQYDGQVIGTLSIA"
     gene            4397..5614
                     /locus_tag="POB34_004046"
     CDS             4397..5614
                     /locus_tag="POB34_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI5920902.1"
                     /translation="MPTEYARDNLGRYQTDGLSAKDFNKVFDLIRKQQRQNRRNARRT
                     LTPRIMGMRNRELEAFLSLGKKKDGTYFTPEDIRSFNTSRQAHKTKFKSTVPGITYAQ
                     LVAQSTSIDIKRANNKVSDGTGIKAATFLGLKHNLALISVNASDESVHQHHRVRIRFE
                     EWDKAVEDIAEDGAKKARIAADLCKGRVSFDCDCGRHQYWYRYMATAGNYAVAPPKEY
                     AFPKIRNPDLTGVACKHVLHAMTRFQSPTWHKAIIIALEKAAEQVAFGDDKRKTTTYF
                     KGELAKSLARNRTTTTDQAKAAREYELYLKSQDALGKKLRAKDSATDNVRRLLKKART
                     TANRKNAELKASRVREAQARAEADALKKALQTQANNLIKFFMSQGMDKAAATAQARSI
                     LETQINEARKRKG"
     gene            5618..6346
                     /locus_tag="POB34_004047"
     CDS             5618..6346
                     /locus_tag="POB34_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI5920903.1"
                     /translation="MAGFFDDMFEDTEPSQQVTGDNLPDTESDPDIPGEGSELIEEED
                     IDAEIETDGVNVGNIVDPVEDNHLPNLDHGLLSDSGVRHRYQGHAVFNNLVRMDWLKA
                     IKLDPDSFDAVLYRAIPYRDKNAPETASEIIEPNQRIYDYQDPELITALDCPDEMDAF
                     YGLYDGSDNTGISDSALILRLAAVNVPVGSMLEWLEQLSDGTTIRRFWYIHKIFNYGT
                     ARVGSLFYCVPSRAFEGNFIGDSE"
     gene            6333..7118
                     /locus_tag="POB34_004048"
     CDS             6333..7118
                     /locus_tag="POB34_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate"
                     /protein_id="HDI5920904.1"
                     /translation="MILNNQEWLLAIFKKKGLTPTGKLEFATIDGIDSALAQALNEAF
                     DSQVVSFNDRANQSFREFLKRTPRDRITLGTFSDVKEWLSSFEADRAGRKDTVSSGPV
                     NKLAMPLVNLSRSPAFSIYEGELCRDNYEEGQVTNENDEIEALVSTIPFSLEYSLWIA
                     SDEKESLGMVSTALAFWLRMYASLGQASFTHIANVGGYEIPVTCYIEGQKSIAFQDLT
                     TGTADNRLFAVGLNLTVVAELPILAYMKQTNGTITVKAKILEA"
     gene            7120..>8070
                     /locus_tag="POB34_004049"
     CDS             7120..>8070
                     /locus_tag="POB34_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDI5920905.1"
                     /translation="MATKTTTAPETDSKRTQLFLQSVSIGQNEIPREMIVGCTYVEPG
                     ELSGPQLMLMVRDSTAYVVNKLGVKFGTILTVSLGDPEGHGGILFSEEFFVLKAPRKD
                     DTVLIYAFSNPVRLLKVPSTSAQYFVDKPPSAVVSSLAPGLKVNADSFRKTSTYHLNV
                     GEKPTKVLQEIARDTGSMCWASRGTINFKSMEKMANAAPSLTYESANPNTSGFTISQF
                     NILNADYEYQRRHNYRMASYDMTKGVVYSGNQEDPIKFTSNPDPTALANYNKFILPRL
                     DMLVEGNAALTPGTTLKIVVHNTAGDGELDESIPDKMIVMS"
BASE COUNT         2156 a         2059 c         1848 g         2007 t
ORIGIN      
        1 ctgaagtgct catttcaaag ttccgtatta gcttgtgctt cggtcatttt tcctaagtat
       61 gaaggaggaa ggactacgca atttgtatcc agtccctcac ctatggcagc ctgtaaaatt
      121 ctggctaagg tgagtctctt gttgcgatac ctggtgatga catgcctgat accgccggtc
      181 ggcgtaacaa aggcgatcag ccagtagtga tatttccgtc ggaatggcca catagtgcac
      241 cttgtagatt gctctaataa aaaacgtgat gagtgtacat cacgttttaa aaatatggaa
      301 ttattagagc aatattattc tggctcttgc tcaaaaaacg cgctaatgaa ggggagccaa
      361 tcctctgaca cttcgcgagg tcgcggtttg ccgtggaaaa agattattcg gcagtctttt
      421 ggtaatgccc cattccccct ggagtaacgc gcgctcgcat atttcgaacc aggttccaca
      481 acatcggcct tgtaacttac aaaccatccc ggatacagat cctgaaatgc tggtgtatca
      541 tcacccataa ccttccgcaa aaacccctga tcaccccagc attcagtagt aacacaacga
      601 gcaatccaac cttccggatc ttgccagaat gcactccaga tatgcgcttt tacactattt
      661 ggtatccaca gggcaccgct accacgatat tgtggatggt aaaaatccct aagcatggtg
      721 aagctggttg gtggattctc aaggattggg cgtatatcac cggcaataac cgtgtccaaa
      781 tccagataga acagatcatc ggttatatcc ggtcggaaca actcgatttt cgcccaccag
      841 ccacggcact tttgccactg gttgatcaat gggacaactt tgacgccagg tacatgtaaa
      901 cgcttcaggt ctgtcaggca aataatttca tagccttttg gcagttgatt aaccagccac
      961 tgcacatcgg aagcgttata gtcaccacca gagcgaaaaa ctaaagcaat cttcatgctg
     1021 caccatcacc tttcactttc atcaatgtca ggtttccgca aaatacggca ccagtgtcga
     1081 tatactgctg attccagaat gtcttcgggc ttttcaccgg agtgtgacca aagataaaac
     1141 gatctgcgcc cgaaatttcg ccaccaatat catccatcga atcactgata cgctcgcgcg
     1201 cccagacaac gttgaaaagc ggcacctcct taccgaattg gtattcatta tccggatagt
     1261 cggcatgggc tataacgata gtttcttgcc cggtgttcaa ctcaatgata tagggcagac
     1321 gctttaccag ctccaccagc gcccaggcta atatttcctg atcagtgtcc agcatgaaga
     1381 accattgtcc gccattcatt agccagttat tcacgttgcc atctggactt aacgcatcaa
     1441 tcatcagccg ctcatggttc cccatcactg ccctgaacca gggcatctgc aatagttcca
     1501 gacattcgac attttcagta ccgcgatcga taaggtcgcc gaccgatatc agtaaatcct
     1561 gcgccgggtc aaaatccaca cgatggagtt cggacatcag tctggtgtag caaccatgca
     1621 gatcaccaac aacccagaca ttcctgtatt tggtaccgtc gatacggtga taaattgtgg
     1681 gtgccatcat gtattcttca gccattcttt aagagtcatc tgcggaatac ctcccatttt
     1741 cccgcatgaa acaacgtcaa tctgttcacg cgcagactgg aataacaaag gcaggtgact
     1801 tagatttttt ggcgtgccgc cggagtgaac gcgtggttct tgtgtagcgt caacgcccac
     1861 cagggcgaca tgtttgaatc cgatatggaa agccaggttc agagcaccat atgcactatt
     1921 gccgctggca atttcattct catcttcgca aaggccgaaa tgtgcggacc agcgccacgc
     1981 ccaccactcg ggagaattcg tattttttgg ctccatgccg cgttcagcca cacgacggaa
     2041 gcacagaacg ccatctctga cttcacgttc tttaacatcg ggtagtgcca tgcaataaca
     2101 aacaccacgg cgacggcggc cacgaccaac gcgccgcata ttgtctgggg atggatcaag
     2161 ggtgaaaaaa taagaagcgc ggttaagcca gtcgatggcc ccattgaccg ctataatcgg
     2221 cactccgcgc ggcgcaacaa agtttgcggc gcttgggcca ctgccgacga taataacgcg
     2281 atcactgcct ctaaatttat tcttgggaaa cattgaattg cactgctcct acttgcattc
     2341 aaaatatgta aatctgcgtg ttttttgcgg gtatccagga actgctgttg ccattttgaa
     2401 atagacacct gcgttggatt ccgtagggct tgagggtgcg ccccatgcca atgaaggccg
     2461 ttttgcagag aacagtcata gccgactaat accactactt cagcccctga ttcagcagcc
     2521 agactgatag cctgcgcgcc gctatttacc ccttccgccg gtccacaata tcgcctgtac
     2581 tccaacgaaa atgatttcgc cgccgccagg ttggctgtca ctttgcggaa tctccctccc
     2641 ggtatggtgg atccgtattg cttccaccat gacaaatcac cggcgtataa ggcataaatg
     2701 tcatcgaaca tctgccagga attgttaacc gcgatgattg aacagccagt tttttctata
     2761 gcagcacagt cctcacgagt gagtgacgga ccgctaccga cacaaaaaac agtcctagtc
     2821 gccctgggtg gtatgttcat tctcagctgc aaattcagcc tccaggcgag cattcatttc
     2881 agcgattaca gggtccacta cagcatctgt ttcctgttca ttacgcggca tgaccgatgc
     2941 cagcgactca taattaacct tggatgacac gattattctc ccgatgttaa agtgcactac
     3001 cacaaagagc gtacatgcac taattaattt attattttaa gcagcataca accacttgtc
     3061 gccgttcaat acatgctcaa tagcctcacc ctttttaagg cttatgtatt ccaggatggc
     3121 ggttatcgct tgttctgcac catacgcaag aacgacgtag taaccttcct ctctaagcct
     3181 gcgcatccag gcgatctgct ctttcgtcgg ggctttacca tttggttctt taagctcaat
     3241 tcgcatgccg tgataaatac cgcatgcttt atcgagactc atgtccggat aacctttttt
     3301 ctgcccttca gccttcattt tcccggcggt tgcttttgaa cgtttccctc cgttaggcgt
     3361 tgcatgcaac agctcataga tgtcagggtg cttgcgttcg aagtaatcaa aaatgaaaac
     3421 ctgctcgaag tgctcgcaat ttccgtcgcg caggtctggg ttctttgcaa gtgctgcaag
     3481 tgccttcgca tgtggagaaa cttcttttac cggcgcaagc gataagaatg gatccttttt
     3541 ggtttttggc ctggaccgcc ccttatttcg acgctcacta aaagcctgaa actcttcctc
     3601 agtaaagcgc aacataatca gtcaaatcct gccggtcgca tgccatattt acgctgtttt
     3661 gcggcctgct cttccctgtg ccattgcgca cattcagcgt cacaataaat gcctgattca
     3721 atcgattcat tgcagtaacg acacttccct gtaaatacct ggctcacgac ctgtgcctgc
     3781 tttctgatgt tatcgatggc catgtctttg agagcttcta actgattcat gctcagctct
     3841 gcatcatcaa cacgttctgc caattttgtt tcctcgtgaa gaacctactt aagggcagaa
     3901 tgatacattt cacaatcaaa attgcactaa taattttctt ttattgagtt aaatattcaa
     3961 caaatgacta gcggtagaat caccatcatc tatttctggc aggctgacta tggctacatc
     4021 aatcactaca acccaaagca cccggcaata tcctctgtcg cggtatgacg accgcaacat
     4081 agccgatcca atactcaggg cagagctacg caaagaggtg atgcttatgt gtgaatcgaa
     4141 cgacaagaat ctgacgattt attacgttct tcccgatgag caatatcgcc cggatttgct
     4201 ggcttaccgt atgtggggca tagcagagct acgctgggtt gtgacgctcg ccgccgggct
     4261 tgaggatgag tctcagggta tgactgttgg caaaaaatta aaactaccac ctgccacatg
     4321 gatccgcgaa atgattcgcc atttccaata tgacggccag gtgataggga cattatccat
     4381 tgcgtaaggg aaatgaatgc caactgaata tgctcgcgac aaccttggtc gctatcagac
     4441 tgatggatta agtgcaaaag actttaacaa ggtcttcgat cttatccgta aacagcagcg
     4501 tcagaatcgg cgaaacgcgc ggcgtacact caccccaagg attatgggga tgcgtaaccg
     4561 cgaacttgag gcattcctca gccttgggaa aaagaaagat ggcacctact ttacgcccga
     4621 agatatacgc agtttcaaca cctcaaggca ggctcataaa acaaaattca agagcacggt
     4681 acccggcatt acctatgctc agctggtggc gcagtccacc agcattgata taaaacgcgc
     4741 taacaacaaa gtttctgatg gcacagggat caaagccgcg acattcctcg ggctaaaaca
     4801 caaccttgca ttgatatctg ttaatgcctc ggatgagtcg gtccaccagc atcaccgtgt
     4861 cagaattcga tttgaggaat gggataaagc cgttgaggat attgctgaag acggtgcgaa
     4921 aaaagcccga atcgctgccg atctctgcaa gggccgggta tctttcgact gtgattgtgg
     4981 acgccatcaa tactggtatc gttatatggc cacggctggt aactatgctg tcgcgccgcc
     5041 aaaagagtat gcattcccca aaatccgcaa ccctgatctg actggtgtgg cttgcaaaca
     5101 tgttttgcac gctatgacgc gttttcagtc tcccacatgg cacaaggcca tcattattgc
     5161 cctggaaaaa gcagctgaac aggtggcctt cggcgatgac aagcggaaga caacaaccta
     5221 tttcaaaggc gaactggcta aatcgctcgc gcgcaaccgg acaacaacga cggatcaggc
     5281 taaagctgcg cgtgagtatg agctgtatct gaaatctcag gatgcattag gcaaaaaact
     5341 acgcgccaaa gatagcgcca cggacaacgt tcgccggttg ttaaaaaaag ctcgcaccac
     5401 ggcaaacagg aagaatgccg aactaaaagc ctcgcgggtg agggaagccc aggctcgcgc
     5461 tgaagccgac gctctcaaaa aagctctgca aacgcaggcg aacaacctca taaagttttt
     5521 catgagtcag ggaatggaca aggccgctgc cactgcgcag gcgcgaagca ttcttgagac
     5581 acaaattaac gaagcccgta aacggaaagg ataatcgatg gcaggtttct ttgatgacat
     5641 gtttgaggac acagaaccat cacaacaagt gactggtgat aacctcccgg acaccgaatc
     5701 ggatccggat attccaggcg aaggttctga actgattgaa gaggaagata ttgatgctga
     5761 aatcgaaacc gatggtgtta acgttggtaa tattgttgat cctgtggagg acaatcacct
     5821 tcccaatctg gatcacggcc tgcttagtga ttctggtgtg cgccaccgtt atcaaggtca
     5881 tgcagttttt aataaccttg tgcggatgga ctggctcaaa gcaatcaagc tagacccaga
     5941 ctcattcgat gcagttctgt atcgcgcaat accttacaga gacaaaaatg cacctgaaac
     6001 ggcatctgaa ataatagaac cgaaccaacg catatatgac tatcaggatc cagaactgat
     6061 aacggccctc gactgcccgg atgagatgga cgccttctac gggctatacg acggcagtga
     6121 taatacaggg attagcgaca gtgctttaat ccttcggtta gccgccgtta atgtgccagt
     6181 gggttctatg ctcgaatggc tggaacagct gtcagacggc acaaccattc gccgcttctg
     6241 gtacatccat aaaatattca attacggcac tgccagggta ggcagtttgt tttattgcgt
     6301 gccttcacgc gcctttgaag ggaatttcat cggtgattct gaataatcag gaatggctac
     6361 tggccatctt taagaaaaaa ggtcttactc caaccggtaa gctggaattt gccactattg
     6421 atggcattga ttcggcgctc gcacaggctt taaacgaagc gttcgactca caagttgtca
     6481 gctttaatga tcgagctaac cagtcgttcc gggagtttct gaaacgcacc ccaagagatc
     6541 gcataacgct cggcactttt agtgatgtga aagaatggtt gtcatcattt gaagccgatc
     6601 gcgccgggcg taaagataca gtctcttctg gcccggtaaa taagctggca atgccgcttg
     6661 tgaatctgtc tcgttctccc gcgttttcaa tttatgaagg tgaactgtgc cgcgataatt
     6721 acgaggaagg acaggtcacc aatgaaaatg atgagattga agccctggta tcgactatcc
     6781 ctttctcact ggaatattcg ctatggatag ccagtgacga gaaggaatct cttgggatgg
     6841 tttcaactgc attagcattc tggctacgaa tgtatgccag cctcgggcag gcatctttca
     6901 ctcatattgc caatgtcggc ggttatgaga taccggttac ctgttacata gaagggcaaa
     6961 aatcaatcgc atttcaggat ctgaccaccg gcaccgccga taacaggctg ttcgcggttg
     7021 gattgaacct caccgttgtg gcggagcttc caatcctggc ttatatgaag caaaccaacg
     7081 gcaccataac ggtaaaagcg aaaattctgg aggcgtgaga tggccacaaa gaccaccacc
     7141 gccccggaaa ctgattcaaa acgcactcag ctattcctgc aatctgtttc aattgggcag
     7201 aacgaaatcc ctcgcgaaat gatcgtagga tgtacctatg tcgaacccgg ggagctatct
     7261 ggtccccagc ttatgctcat ggtcagagat tcaacggctt acgtggtcaa taagctgggg
     7321 gtgaaatttg gtacaatact gacagtttca cttggtgatc cggaaggtca tggcggcatc
     7381 ctcttctcgg aagagttctt tgttcttaaa gcgccgcgca aggacgatac tgtactgatt
     7441 tacgcgttta gtaacccggt gcggttatta aaagttccgt ccaccagcgc acagtatttt
     7501 gttgataagc ccccatcagc cgtagtttcc tctcttgccc ctggtctgaa ggttaatgct
     7561 gactcattca gaaaaacatc cacataccac ctaaatgttg gagaaaaacc gaccaaggta
     7621 ttgcaggaga tagcccggga taccggttct atgtgctggg catccagggg gacgatcaat
     7681 tttaaaagta tggaaaaaat ggcaaacgcc gctccatcgc ttacttatga gtccgccaat
     7741 cccaacacat ccggatttac aattagtcag ttcaacatcc tgaatgccga ttatgaatac
     7801 cagcgccgcc acaattacag aatggccagt tatgacatga ccaaaggtgt ggtttactca
     7861 ggtaaccagg aagaccccat taaatttacg agcaatcccg atcctaccgc gctggcgaac
     7921 tacaacaaat tcattctccc ccgcctcgat atgctggtgg aaggaaatgc cgcgctaact
     7981 ccgggtacga cgctgaaaat tgtcgtgcat aacacggcag gtgacggaga actcgatgaa
     8041 tctatccctg acaaaatgat agtgatgtcc
//
LOCUS       DAMZET010000182         7938 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.182, whole genome shotgun sequence.
ACCESSION   DAMZET010000182 DAMZET010000000
VERSION     DAMZET010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.182"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..594
                     /locus_tag="POB34_004050"
     CDS             <1..594
                     /locus_tag="POB34_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDI5920906.1"
                     /translation="HCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALWHFLD
                     SLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHAARPHSEAIRD
                     AQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHT
                     PDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            647..1042
                     /locus_tag="POB34_004051"
     CDS             647..1042
                     /locus_tag="POB34_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HDI5920907.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIALMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            1083..1826
                     /gene="cmoA"
                     /locus_tag="POB34_004052"
     CDS             1083..1826
                     /gene="cmoA"
                     /locus_tag="POB34_004052"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HDI5920908.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1823..2794
                     /gene="cmoB"
                     /locus_tag="POB34_004053"
     CDS             1823..2794
                     /gene="cmoB"
                     /locus_tag="POB34_004053"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HDI5920909.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2959..5388)
                     /gene="torZ"
                     /locus_tag="POB34_004054"
     CDS             complement(2959..5388)
                     /gene="torZ"
                     /locus_tag="POB34_004054"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HDI5920910.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTTRIQHPMVRKSYLDNPLQPT
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIDFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLRGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRAIPVARIVDVLENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAGLRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5413..6513)
                     /locus_tag="POB34_004055"
     CDS             complement(5413..6513)
                     /locus_tag="POB34_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDI5920911.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGAD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(6901..7647)
                     /gene="cutC"
                     /locus_tag="POB34_004056"
     CDS             complement(6901..7647)
                     /gene="cutC"
                     /locus_tag="POB34_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDI5920912.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7661..>7938)
                     /locus_tag="POB34_004057"
     CDS             complement(7661..>7938)
                     /locus_tag="POB34_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI5920913.1"
                     /translation="LPWPGEKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLP
                     GISVKTSSPKGEHERLPNPTLAVTDGKTTIKFHPWSIEEIVASEQSA"
BASE COUNT         1836 a         2080 c         1882 g         2140 t
ORIGIN      
        1 cattgcgatg atttagtgac tgaatttttg acccgcatgt caccgttggc tccgcgcatt
       61 gggcaatact ggctgcaact gcctgccaca ttcggcccac gggagctgcc tgcgctttgg
      121 cattttctcg attctctccc tggcgaattt aattatggtg tggaagtccg ccatccacag
      181 tttttcgcca aaggggaaga ggaacaaacg cttaatcgcg gtttacatca gcgcggcgtt
      241 aatcgggtga ttttagacag ccgcccggtt catgcagcac gtccacacag tgaagctatt
      301 cgcgacgctc aacgaaaaaa acctaaagtt ccggtacatg ctgtactgac ggcgacaaat
      361 ccgctgatcc gttttatcgg tagtgatgat atgacgcaaa accgggaatt atttcaggtc
      421 tggttacaaa aattagcgca gtggcatcag accactacgc cttatctttt tttacatacg
      481 ccagacattg cccaggcccc ggaactggta cataccctgt gggaagactt acgtaaaacg
      541 cttccagaga tcggagcagt tccggctatt ccacagcaat cttctctttt ctgaatttgc
      601 cacctatcat agacaggtgc catcggccat tttaaaggga gtttgtatgg taagcgcgct
      661 gtatgccgtt ttaagtgcgt tgttattaat gaagttctct tttgatgtcg ttcgcctgcg
      721 aatgcagtac cgcgttgcct atggtgacgg tggttttagc gaactgcaaa gcgctattcg
      781 cattcatggt aacgcggtgg aatatattcc catcgcgatt gcgctgatgc tgtttatgga
      841 aatgaatggc gcagaaacct ggatggtgca tatttgcggc atcgttttgc ttgctggtcg
      901 tctgatgcat tattacggtt ttcatcaccg tctgttccgc tggcgacgtt ccggcatgag
      961 cgccacctgg tgtgcgctgt tgctgatggt gctggcgaat ctttggtata tgccctggga
     1021 gttggttttc tccctgcgtt agcgcacaat acgccacttt ctttttcccg gatttttacg
     1081 ttatgtctca ccgcgacacg ctattttctg cccctatcgc cagactgggc gactggacct
     1141 ttgatgaacg ggtagctgaa gtcttcccgg atatgatcca gcgttccgtt cccggctatt
     1201 ccaatattat ttccatgatt ggtatgttag ccgagcgctt cgttcaacct ggtacgcagg
     1261 tttacgatct gggttgttct ctgggcgcgg cgacgctctc ggtgcgtcgc aacattcatc
     1321 atgataattg caaaattatt gccatcgaca actccccggc gatgattgaa cgctgccgtc
     1381 gtcatattga cgcctataaa gcccctacgc cagtagacgt cattgaaggt gatattcgcg
     1441 atatcgccat tgaaaacgca tcgatggtgg tgctgaattt taccctgcaa ttcctggaac
     1501 cttccgagcg ccaggcatta ctggataaaa tttatcaagg actgaacccg ggcggcgcgc
     1561 tggtgctttc ggaaaaattc agtttcgaag atgccaaagt tggtgaactg ttgttcaaca
     1621 tgcaccacga ctttaaacgt gccaacggtt acagcgaact ggagatcagc cagaagcgca
     1681 gcatgctgga aaacgtgatg ctgaccgatt ccgtggaaac ccataaagca cgcctgcata
     1741 aagccggttt tgagcatagc gagctgtggt tccagtgctt taactttggt tcactggtgg
     1801 cattaaaagc agaggacgct gcatgatcga ctttggtaac ttttattctc tgattgccaa
     1861 aaatcatctt tcacactggc tcgaaacgct gcccgcgcag attgctaact ggcagcgcga
     1921 gcagcagcac gggctgttta agcagtggtc caacgcggtg gaatttctgc ctgaaattaa
     1981 accgtatcgt ctggatttat tgcatagcgt aaccgccgaa agcgaagagc cactgagcac
     2041 cgggcaaatt aaacgcattg aaacgcttat gcggaacctg atgccatggc gcaaagggcc
     2101 gttctcactg tatggcgtca acatcgatac cgaatggcgt tccgactgga aatgggatcg
     2161 tgttctgccc catctttctg atttaaccgg acgcaccatt ctggatgtcg gctgcggcag
     2221 cggttatcac atgtggcgca tgattggcgc aggcgcgcac ctcgcggtgg ggatcgaccc
     2281 tacgcaactg tttctgtgcc agtttgaagc ggtgcgtaaa ctactgggta acgatcagcg
     2341 cgcacatttg ttaccgttag gtattgaaca gcttccggca ctgaaagcct ttgataccgt
     2401 cttttcgatg ggcgtgcttt accatcgccg ttcgccgctg gagcatctct ggcagttgaa
     2461 agatcaactg gtgaatgagg gtgaactggt gctggaaacg ctggttattg atggcgatga
     2521 gaacacggtg ctggtaccag gcgatcgtta cgcgcaaatg cgtaatgtct atttcatccc
     2581 ttccgcgctg gcgctgaaaa actggctgaa gaagtgtggt tttgtagata tccgcattgt
     2641 ggatgtgtgc gttaccacta ctgaagagca gcgacgcact gaatggatgg ttaccgaatc
     2701 tctgtccgat ttcctcgacc cacatgatcc gagtaagaca gttgaaggtt atcctgcgcc
     2761 taaacgcgcg gtgctgattg cgcgcaagcc gtaaaggtct ggtattactg ccggatgcgg
     2821 cgtgaatgcc ttatccggcc tacaaaccct tgctaattca atctattgca gggactatgt
     2881 aggcctggta agcatagcgc atcaggcagt tttacgtttg cataacctca gcgcccgttt
     2941 ccgggcgcta ttcacttctt acgcctgaac agcaggctta tcaaatgccg ttaacttcgg
     3001 tgcgttaccc gtatattttt cgatatacac cagcgcagag ttaccggcac aggcatttgc
     3061 cagctgcgag ctggggatat ccgccgttaa cacgttcgca ctgccgtttt tacacaagcc
     3121 gttttccaga tctggccatg caccttcatg cacgcatacc acgccttttt tgatcccgtc
     3181 agtcaccacc gcgcctgtca aaatctgacc acgtttgttc cagacacgca ccagatcgcc
     3241 attcgcaata ccaaatcgag cagcatcttc ggtgtgaata gtgatcggtt cacgatcggc
     3301 aaccgcatat tttttacgca gtcccgcata gttaagctga ctgtgtaaac ggtgtgccgg
     3361 atgtgcggtc agaagctgca actgcttctc gtcggcggta cccttccact catcaggcgc
     3421 aagccaggtt gggtgtgccg ggcaatcctt atagccaaat ttttccagcg ttttggagta
     3481 aatctcaatt ttgccacttg gcgtacccag cgcatttttc accggatcgg cgcggaaatc
     3541 accataacga acgtactgtt cgttcttctc gctgcggcgc atttcgatca gtttattttg
     3601 ctgccagaag gcattaaaca ttggcatagt gacgcgttgc gcacgggccc ctttctgagc
     3661 ggcatcatag aaaaatttca gccacgccat ttcatcttta ccttcggtat agatctcttt
     3721 tccgccaggt ttcagtaatt ccgcaagatc ggcaaacaca tcaaaatcgt ttcgcgcttc
     3781 aaattgcgga gcaacagcct gcttcatcgg cacaatatgc tggttgctgt aatcaccggt
     3841 catcgtcaag tcattgcgct caaacgatgt ggtgatcggt aatacgatat ctgcatgttt
     3901 agccgctgcg gtccagtagc attcagaaac gacgatcatc tccggtttct gccatgcttt
     3961 aatcagacga ttagtatcct ggtgatgggt aaagttaccg ccacccgccc accagatcat
     4021 tttgatattc ggatacgttt gctctttacc gttatgctga tatttgccgc caggattttc
     4081 cagcacatcg acgatacggg caacaggaat cgccctcatt ccgccatcat cagcggcctc
     4141 gctggcctgt ccggcaatcg ccgcagacat ttccggcagc acgccgccaa cacgtgtcgg
     4201 gttgccacca tttgagtaat gataagagaa accaaaaccg ccgcccggtg taccaatctg
     4261 ccctaacatc gccgccagcg tcaccagcat ccagtgtttc tgttcaccat attgctggcg
     4321 ctgaattccc cagcctgcca tcagcattgt gcgattcgcc gccatcagtt cagcgagttt
     4381 gactatctgc gcttccggta cgccagtaat ttccgccgcc caggccgcgc tcttcggcgt
     4441 attatcgctc ttacctctca gatactcttc aaactgcgga tacccggtag tgtatttttc
     4501 aaggaacact ttatcgtgtt tgccttgtgt catcagggta tgcgcaatcc ctaacatcag
     4561 tgccacgtcg gtgcccatat tcggcgcgat ccaggtggca ttatcgccaa agaagtcgat
     4621 ggtttcggag cggataggat caatggcaat cactggtttg ccagattttt tcagctgatg
     4681 gaagtattcc agcccttgct catcggtact gctccaggca atttttaagg tattcagcgg
     4741 gttcatcccc cacagcacca caacctggct gttttccaga atcagcggcc aggaggtctg
     4801 ctgttcatac acctctacag aaccgaccac atgcggcatg atcacctgtg ccgccccggt
     4861 tgaataatcg ccgctatgcc cggaataacc gcccgccagg ttcatataac gttgcagtaa
     4921 ggtttgcgct ttatgcaaca cgccagaaga gcgccagccg tatgatccgg caaaaatagc
     4981 cgatggtccg ttagctttgc gaatgcgatc gtgttgctca tgaatcagtt ttaatgcttg
     5041 ttcccagctc acctgtacat aggtatcttc gccacgacct ttcgtcggtt gcagtggatt
     5101 atcgagatag ctttttctca ccatcggatg ctgaatacgc gtcgtggtgt gtacctgatc
     5161 cgccgccgta gactgtaagg aattcggtat ggttttcgcc agcgcgcctg ttgaagaaac
     5221 aatcttgccg tccttcactt ctacgttcat cgctccccaa cgtcccgcgg tgaggatttt
     5281 accgcccttc tcttctgccc atgcggggag cggtgctgcc gatgtcacca ccagcgctcc
     5341 agcggcaata ccgctgtgtt taataaattc acgtcttgtt aatgtcataa cttcctccct
     5401 gatcaacgag gatcactgtt tctcggtaat atctttggcg ttgtactgga aataccgcgt
     5461 taaaatgtcc agttcgtttt cgctcatgct ggttcgtgcc cccattcctt tggcaatgga
     5521 cggccacgca ttgacggtgt aatggtcggc ggcaataggg gcatgacaac cagcgcaata
     5581 ggtatcgtca agtttttcag cgtattgcca taacggttta cggtccgcta atccgggatc
     5641 ggtaagcgca ccctgtaaag acgcctgacg ccattgattg ccgtattcgt cagcctgcca
     5701 ttccccgttt accgtgagcg ccttgatacc ttcttcactt aatgtggcta gcgccagccg
     5761 ttgacctgcc gccaggtaga gagtgttttc actgccctgc atttgataac cctgcaacag
     5821 aacgatcggc tgtttgccac tggcatcaac gacggtgaga tcggcaccag gattcacggt
     5881 agccagctcg cctatgcgag aagttttgaa aggataaata tgtgcgccat tagtcactga
     5941 agtagcggcc tgactttcca gctcatgcgc cgcgttgtca tccattttta tttctggcgg
     6001 aaaatgggca atgcctttat gacaatcgat acaggtttcg ccgccctttt gtgctttgtt
     6061 atgcattttc tgcgcagatt cactttgcga ggcaatatcc atggcatcaa aagaatggca
     6121 actacggcac gttgcagagt cagttgcttt taattctttc catactgttt cggccatttc
     6181 ctggcgatga gtttcgaact tatcgtcact gtctattttg ccgctaacaa attcatgata
     6241 aatatcttta gatgctttta atttagcaaa taaataatcc atccctgact ttggaatatg
     6301 gcaatcggca cattccgcac gtatcccttt ctggttcgaa aagtggacag ttccctgata
     6361 ttcctcaaaa ggtttactca tcgagtggca ggaaaggcaa aatgctgtat ccgacgtttt
     6421 atgtaaggct ttttgcgcca gcaataaccc gccgccacca accacaaccg ctatcagcaa
     6481 aaacaataac ccaatgcgtt ttttccctcg catagattcc tctaaataaa ttccagaaca
     6541 ccgaaatcat tgtataatta aatacacaac gaattaaccg cttgcataac tcggcacaac
     6601 actgcctgaa ataatcgata aagaatatga tttattacaa tgtaatcatt aattgctaag
     6661 gaataaccca gttgctattt agaatatata tttatcagtt ttagcaattt aaatcccata
     6721 atcaatgtga atataaaaaa tattattagc acaacgctat gttcactgtc acccgctcaa
     6781 cagatcacgg ttatcgttcg ttttttatac tgctcaggga taaaaaaagg cccctgttga
     6841 aattgcaggg gcctggtacg agcaagcatc atattgggcg acatgatgca acggtaaaaa
     6901 tcatttggcc tgatggcgtt caatgattcc tttcatttca gcaaccgccg ccccgtctac
     6961 gatataacgc gaatactcgt ccgcgtgttc atctgatgac atggacaatc cttgattacg
     7021 ataacgcatc ggtgaggctt gccacgctcc cgcggagcta tggacttcca gcactccggc
     7081 atcgaggaag tggtgcaggt tttctgcacg gactcctgct ccggccataa tgattggagc
     7141 atcacgatgg gcaataagtt ccataatttt tgataaacct tgcagtgcgt ctgatttttg
     7201 ccctgatgtc agtacccggg caatgcccaa ttccgcaaga ttattgagtg tatataaagg
     7261 gttagcgcac atatcgaagg cgcgatgaaa agtcactgcc agcggaccgg cagcagccat
     7321 tattttttcc attcgtggca tatcgacatt cccgtcaaca tcgagaacgc ccgtcaccag
     7381 tccaggaaaa cctaattcgc ggaccgtgcg cacatcctca agaatggcgg caaactcacc
     7441 gtcgctgtag caaaaatcac caccgcgtgg gcgaattatc ggatgcacag ggatcgtcac
     7501 ccgctggcgc acggatttca gtacccccaa cgacggcgtt aagcccccct cttttggggc
     7561 tgcgcataat tcaactctgt ctgcgccgtt ttgctgcgcc gttagtgcac attccatgct
     7621 gtaacagcaa atttccagta atgccatttt tgctccttaa ttacgctgac tgctcgctgg
     7681 caacgatctc ttcaatggac cacggatgaa acttaatagt tgttttgcca tcggtgaccg
     7741 ccagcgtcgg attcggtaat cgctcatgct caccttttgg tgaactggtt ttaactgaga
     7801 tccccggtag gctgagtcct tcatcagaaa gcaaagccag cgcacgggca ttcagcgttt
     7861 ctggatcacc cggcagaacg atttcaatat gttcccaacc ttcgtgtggg taacgttttt
     7921 ccccgggcca cggtagct
//
LOCUS       DAMZET010000183         7931 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.183, whole genome shotgun sequence.
ACCESSION   DAMZET010000183 DAMZET010000000
VERSION     DAMZET010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.183"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..283)
                     /locus_tag="POB34_004058"
     CDS             complement(<1..283)
                     /locus_tag="POB34_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HDI5920914.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIV"
     gene            709..2367
                     /locus_tag="POB34_004059"
     CDS             709..2367
                     /locus_tag="POB34_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HDI5920915.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2364..3320)
                     /locus_tag="POB34_004060"
     CDS             complement(2364..3320)
                     /locus_tag="POB34_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HDI5920916.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3471..4586
                     /gene="macA"
                     /locus_tag="POB34_004061"
     CDS             3471..4586
                     /gene="macA"
                     /locus_tag="POB34_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HDI5920917.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQELDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4583..6529
                     /gene="macB"
                     /locus_tag="POB34_004062"
     CDS             4583..6529
                     /gene="macB"
                     /locus_tag="POB34_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HDI5920918.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDYFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6603..6827)
                     /gene="cspD"
                     /locus_tag="POB34_004063"
     CDS             complement(6603..6827)
                     /gene="cspD"
                     /locus_tag="POB34_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HDI5920919.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7150..7470
                     /gene="clpS"
                     /locus_tag="POB34_004064"
     CDS             7150..7470
                     /gene="clpS"
                     /locus_tag="POB34_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HDI5920920.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7501..>7931
                     /gene="clpA"
                     /locus_tag="POB34_004065"
     CDS             7501..>7931
                     /gene="clpA"
                     /locus_tag="POB34_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="ATPase and specificity subunit of the ClpA-ClpP ATP
                     dependent serine protease; directs protease to specific
                     substrates; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HDI5920921.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTR"
BASE COUNT         1960 a         1886 c         2151 g         1934 t
ORIGIN      
        1 caacaatacc gccgacaacc tgggcaatta cgtagccaac gacttctttt gccggaaaac
       61 gtccgccagc ccataaacca atagtgaccg ccgggttaaa atgaccacca gaaatatgac
      121 caacagcaaa ggccatcgtc agaacggtca gaccgaacgc caacgccacg ccggcaaaac
      181 caatgcctaa ttccgggaag cctgcggcca gtacagcact accacagcca ccaaaaacaa
      241 gccagaaagt accaaaacat tcagctgcta attttctgaa catatccacc tcaattaaaa
      301 attgaccctg tgaaaaatat ggtcgtttta tagggtcgtc gtaaaaaagt gacgacggaa
      361 ataatgcgcg gctattttaa aaacgaaggc gagtcattca ccagataaat aaatccagta
      421 aatttgattt agggcaacag cgggttgccc catatagtca tttgtctgat tgacagtgta
      481 gtgcaagcaa aagatttaat cctttaggcg taataaaaaa taatttatca tgctaattat
      541 ttgattttgt tgtttttgca gacttatcag caagagggag tataacgcga ttattcgctc
      601 atttttcaga catttgccat gcttaaatgt gatgtcatca cgtattagca aggcctttcc
      661 cgttatactg ccagcgtaaa ggataagtca catatttctg gaggggatat gattcttgag
      721 cgcgttgaaa ttgtgggttt tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac
      781 aacgtcctga ttggggagaa cgcgtggggt aaatccagct tgctggacgc cttaaccctg
      841 ctgctatcgc cagaatcaga tctctaccat tttgagcgcg acgatttctg gttcccgccg
      901 ggagatatca acgggcgaga acatcatctg catattattt tgaccttccg cgaatcgctg
      961 ccaggccgac atcgggttcg ccgttatcgg ccgctggaag cgtgctggac gccatgcacc
     1021 gatggctatc accgtatttt ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg
     1081 atgacactgc gcagttttct cgataaagac ggacatccga ttgatgtcga ggatattaac
     1141 gatcaggcac gccatctggt gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt
     1201 atgcgccgta ttcgtaacgg cacggtgcca aatgttccca atgtggaagt caccgcgcgc
     1261 cagctcgatt tcctcgcccg tgagttgtcc tcacatccgc aaaatctctc tgatggacag
     1321 attcgtcagg gactttccgc aatggtgcag ttgcttgagc attatttctc tgagcagggg
     1381 gccggacagg cgcgatatcg tttaatgcgg cggcgggcca gcaatgagca acgaagctgg
     1441 cgctatctgg acatcatcaa ccggatgatt gaccgacctg gagggcgctc gtatcgggtt
     1501 attttgctcg gcctgtttgc tactttattg caggcaaaag gcacattgag actggataaa
     1561 gacgcgcgtc cattgttgct gatcgaagat ccagaaaccc gtttacaccc cattatgctt
     1621 tcagttgcct ggcatctgtt gaatcttctg ccattgcagc gcattgccac caccaactcg
     1681 ggtgagttgc tttcgttaac gccggtagag catgtttgcc gactggtacg tgagtcctcg
     1741 cgcgttgccg cctggcgtct ggggccgagt ggcttgagta ccgaagatag ccgacgcata
     1801 tcctttcaca ttcgttttaa ccgtccgtca tcgctgtttg ctcgctgctg gttgctggtg
     1861 gaaggggaaa cggaaacctg ggttatcaat gaactggcgc gccagtgtgg acaccatttc
     1921 gatgccgaag ggatcaaggt cattgagttt gcccagtccg ggctaaagcc actggttaaa
     1981 tttgcccgcc gaatggggat tgaatggcat gtactggtcg atggcgatga agcagggaag
     2041 aaatatgccg ctacggtacg cagcctgttg aataacgatc gggaagccga acgagaacat
     2101 ttaacggcgt taccggcgct ggatatggaa cattttatgt atcgccaggg attttccgat
     2161 gtgttccacc gcgtggcgca aatcccggaa aatgtaccga tgaatctgcg caaaattatc
     2221 tcgaaagcga tccatcgctc ttccaaaccc gatcttgcca ttgaagtggc aatggaggcc
     2281 ggacgtcgtg gtgtggactc cgtaccgacg ctgctgaaaa aaatgttctc acgcgtgctg
     2341 tggctggcgc gcggtcgcgc ggattaaccg cgaaacatcg tggccatttg tggctgaata
     2401 gcgtcgagca tctcatagcg ccgacggtat tcagcccgtt ttttactggc gatttcggca
     2461 atctcttttc gtgctatctg tgctggaagg cggtaatggc gttcagcatc acatacgccg
     2521 ccaaccgatt cccagaaagc gttgtaatcg gcatggatct tgccttcttt atcacgataa
     2581 cgcaggctgc ggtaaatatg cgtttcattg ctgacggcaa taatctgctc tacctgcaaa
     2641 cgttgggcaa acagacaggc cgcttccatc acgaggcgtt tgggaaatag cccgtggcag
     2701 gctttcgtcg cattctggat ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg
     2761 ccaataaaca tcgttctttt cccctgatat tcacacaggg taaacgtgat ctctgccaga
     2821 ggaatacctt cgctgttgcg aaacaggatt gtgctgtcac cttctttatc cattgagatc
     2881 atcatggtca gctcaagcgt gaactgctcg ccgtttttgc cttccagttt agccagttgc
     2941 agcccggggg tattcaaata taagctgaat tcttccgccg acatacatcc gcggagtaac
     3001 gcataatggt aacgtaacgc ctccagcaat tgcttacggc taagattcgc cgcaaggtaa
     3061 gggcgatgca gacgcacagg cagtcgcggt tggcgcgtta acaacacatt gagattaggc
     3121 cagtgggaaa gttcgttcat ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc
     3181 aaaaatttct ggcgaaaact acggcgatgc cagaatttac ccggccgaca ctgtccacgt
     3241 gccagactaa gaaaaagtga caggctgctg agagattcag atggcgtaaa ggtccgttca
     3301 gttagctgcg acatattcat gaaatcaatg gttatacatg acgtcgattt caccattgcg
     3361 tatcttaacc aaacatcaat agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat
     3421 ttcgttgaag ttaatgaccc ggattggcat atggagtatt cagaatattt atgaaaaagc
     3481 ggaaaaccgt gaagaagcgt tacgttattg cgctggtgat agtcatcgcc ggactgatta
     3541 cgttatggag aattcttaac gcacccgtgc cgacttatca gacactgatt gtgcgccccg
     3601 gtgatttaca gcaaagcgtg ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg
     3661 tgggcgcgca ggtcagcggt cagttgaaaa ctctgtcggt ggcgattggc gataaagtaa
     3721 aaaaagacca gcttttaggg gttattgatc ctgaacaggc tgaaaaccag attaaggagg
     3781 tcgaagcaac gctgatggag ctacgtgcgc agcggcagca ggcggaagcg gagctgaaac
     3841 tggcgcgggt gacgtattcc cgtcagcaac gtctggcaca aacgcaggcg gtttcacagc
     3901 aggagctcga caccgccgcg acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg
     3961 acgcgcaaat caagcgcaat caggcctctc tcgatacggc taaaaccaat ctcgattaca
     4021 ctcgcatcgt tgccccgatg gccggggaag tcacgcaaat caccactctg caaggccaga
     4081 cggtgattgc cgcacaacaa gcaccgaaca ttctgacgct ggcagatatg agcaccatgc
     4141 tggtaaaagc gcaggtttct gaagcggatg taatccacct gaagccgggg caaaaagcct
     4201 ggtttacggt acttggcgat ccactgacgc gctacgaggg gcaaatcaag gatgtactac
     4261 cgacgccgga aaaggttaac gacgctattt tctattacgc ccgttttgaa gtccccaacc
     4321 ccaatggttt gctgcggctg gatatgactg cgcaagtgca tattcagctc accgatgtga
     4381 aaaatgtgct gacgatccct ctgtcggcgt taggcgatcc ggttggcgat aatcgttata
     4441 aagtcaaatt gttgcgtaat ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta
     4501 acgataccga tgttgagatt gtcaaagggc ttgaagcggg cgatgaagtg gtgattggtg
     4561 aggccaaacc aggagctgca caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag
     4621 ctatcctgcc ggtgatgagc aggttgaggt gctgaagggc atcaccctcg atatttatgc
     4681 cggtgagatg gtagcgattg ttggcgcttc aggttccggt aaatcgaccc tgatgaatat
     4741 tctcggctgt ctggataagg ccaccagcgg cacctatcgc gtcgccggtc aggatgttgc
     4801 cacactggac gccgatgcgc tggcgcaact gcgccgcgag catttcggct ttattttcca
     4861 gcgttaccat ttactttcgc atttaaccgc cgagcagaac gttgaagtac ccgccgtcta
     4921 tgctggtctt gagcggaaac agcgactgct tcgtgcccag gagttgctgc aacggctggg
     4981 gctggaagac cgtacagagt attatccggc acagctttcg ggtggtcagc aacagcgcgt
     5041 cagcatcgcg cgggcattga tgaacggtgg tcaggtaatt cttgccgatg aaccaaccgg
     5101 cgcactggac agccattctg gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg
     5161 tgggcatacg gtgattatcg tcacccacga tccgcaggtc gctgctcagg ccgagcgggt
     5221 gatcgaaatt cgcgacggcg aaattgtgcg caatcctccc gccattgaaa aagtgaatgt
     5281 tgctggcggg acggagcctg ttgtcaacac ggtgtctggc tggcggcagt ttgtcagcgg
     5341 ttttaacgag gcgctgacga tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt
     5401 actgaccatg ctggggatta ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg
     5461 tgacgccgcc aaacaaatgg tgctggcgga tattcgttct attggtacga atactattga
     5521 tgtctatccc gggaaagatt ttggcgatga cgatccgcaa tatcagcagg cgctgaagta
     5581 cgacgactta atcgccatcc aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc
     5641 gcaaaacctg cgcctgcgtt ataacaatgt tgatgttgct gccagtgcca atggcgtgag
     5701 cggcgattat tttaatgtct atggcatgac cttcagtgaa ggaaacacct ttaatcagga
     5761 gcagctgaac ggtcgtgcgc aggtcgtggt tctcgacagt aatactcgcc gccagctttt
     5821 cccccataaa gcagatgtgg ttggcgaggt gattctggtc ggcaatatgc ccgccagagt
     5881 cattggtgtg gcggaagaaa aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg
     5941 gctaccttac agcacgatgt ccgggcgagt tatgggccag tcgtggctga actccattac
     6001 tgtcagggta aaagaaggat ttgacagcgc cgaggcggaa cagcaactca cgcgtttact
     6061 ttcactgcgc cacggaaaga aggattactt tacctggaac atggacggcg tcttgaaaac
     6121 tgttgaaaag accacacgta ctttacaact gtttctgacg ctggtggcgg tgatttcgct
     6181 ggtggtgggc ggtattggtg taatgaatat tatgctggtg tcagtgaccg agcggacgcg
     6241 ggaaattggc attcgcatgg ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct
     6301 gatcgaagcc gtactggttt gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt
     6361 aattgctttc accttgcagc ttttcttacc cggctgggag attggttttt caccgttggc
     6421 actgctgctg gcgtttctct gctcgacggt caccgggatt ttatttggat ggttacccgc
     6481 acgaaatgcg gcacgactgg atccagtaga tgctctggca cgagagtaat ttttgagaaa
     6541 aaaaatgcca gccgatcggg ctggcatttt gcctttagga tgtacacaat gagacagaag
     6601 agctatgcga ctgccgcttc tacttcgacg ggcacaataa cactggcgtg attgcctttt
     6661 ggcccctggt ggacatcaaa ctgaacggat tgtccagctt ttagcgttct gtaaccatcc
     6721 atctgaatgg tggaataatg agcgaaaata tcttcgccgc cgccttcagg gcagatgaaa
     6781 ccaaaccctt tggcattgtt gaaccactta acagtaccct tttccatgct tcgacatcct
     6841 tcgcaaatct tatacaagta agatggaata aaccggggtc agagaggggg ctgttcaaaa
     6901 cctcgccaac tctagaaata caatttagag aattagggcg agccgtcaag cgtttgacag
     6961 gggccagggg gcaggtatga atcaaaaatt tgaagcagtt aacgctattg acaggaatgt
     7021 gacagatgtc gctgatgcca acgatagatg atagttatct atcatgtgga gtagattggt
     7081 caggcaaata agctcttgtc agcggcaggg cgttctgccg ataaccgtaa ccgaagatga
     7141 taactgacaa tgggtaaaac gaacgactgg ctggactttg atcaactggc ggaagaaaaa
     7201 gttcgcgacg cgctaaaacc gccatctatg tataaagtga tattagtcaa tgatgattac
     7261 actccgatgg agtttgttat tgacgtgtta caaaaattct tttcttatga tgtagaacgt
     7321 gcaacgcaat tgatgctcgc tgttcactac caggggaagg ccatttgcgg agtctttacc
     7381 gccgaggttg cagaaaccaa agtggcgatg gtgaacaagt acgcgaggga gaatgagcat
     7441 ccattgctgt gtacgctaga aaaagcctga atgcaggcat aaaaattggg ggaggtgcct
     7501 atgctcaatc aagaactgga actcagttta aatatggctt tcgccagagc gcgcgagcac
     7561 cgtcatgagt ttatgaccgt cgagcacttg ttactggcgc tgctcagtaa cccatctgcc
     7621 cgggaggcgc tggaagcgtg ttctgtggat ttggttgcgc tccgtcagga actggaagcc
     7681 tttattgaac aaaccacacc cgttctgcct gccagtgaag aggagcgcga cacacagccg
     7741 acgctgagtt ttcagcgtgt actgcaacgt gcggtcttcc atgtccagtc ctccggtcgc
     7801 aatgaggtaa ccggtgcaaa cgttctggtc gctatcttta gcgaacagga gtcgcaggcg
     7861 gcatatctgt tgcgtaaaca tgaagtcagc cgtctcgatg tggtgaactt tatctctcat
     7921 ggcacgcgta a
//
LOCUS       DAMZET010000184         7919 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.184, whole genome shotgun sequence.
ACCESSION   DAMZET010000184 DAMZET010000000
VERSION     DAMZET010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.184"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..3591
                     /locus_tag="POB34_004066"
     CDS             <1..3591
                     /locus_tag="POB34_004066"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDI5920922.1"
                     /translation="RVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLE
                     LGIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVE
                     CMFAGRLENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDA
                     TLDMLSGRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLP
                     EGEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLP
                     FWRGEGNGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIEEQRNAT
                     GIVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVAS
                     DDGIVARIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPG
                     RTPGHRTPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNG
                     GEIQISDVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQV
                     DPGELLDPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEE
                     VASYLENLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPL
                     RDLFLRYLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFR
                     RLRLRSLQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVM
                     RVIEQLAGVGLPASLWESQILPARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVA
                     LHLQEYAAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLS
                     ALQEALWALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRV
                     SYNTPNLAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQT
                     LCRSMEDSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPA
                     NVWGNLLPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEV
                     FHALTTALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(3642..3989)
                     /gene="grxD"
                     /locus_tag="POB34_004067"
     CDS             complement(3642..3989)
                     /gene="grxD"
                     /locus_tag="POB34_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HDI5920923.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            4323..5138
                     /gene="mepH"
                     /locus_tag="POB34_004068"
     CDS             4323..5138
                     /gene="mepH"
                     /locus_tag="POB34_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HDI5920924.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            5266..5847
                     /gene="sodB"
                     /locus_tag="POB34_004069"
     CDS             5266..5847
                     /gene="sodB"
                     /locus_tag="POB34_004069"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HDI5920925.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(5993..7162)
                     /locus_tag="POB34_004070"
     CDS             complement(5993..7162)
                     /locus_tag="POB34_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5920926.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(7328..7417)
                     /gene="ynhF"
                     /locus_tag="POB34_004071"
     CDS             complement(7328..7417)
                     /gene="ynhF"
                     /locus_tag="POB34_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HDI5920927.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            7716..>7919
                     /locus_tag="POB34_004072"
     CDS             7716..>7919
                     /locus_tag="POB34_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI5920928.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATS"
BASE COUNT         1950 a         2095 c         2034 g         1840 t
ORIGIN      
        1 cgtgtacaaa gtagcgacgt ttttattgcc cgctcacacc acggctccgt ctctaaagaa
       61 caacgagcaa tcaccgaaca ggcgctgaaa tcgggtgaat tacgctgcgt ggtcgcaacc
      121 tccagtcttg aactggggat tgatatgggc gcggtggatc tggtgattca ggtggcaacg
      181 ccgctttctg ttgccagtgg gttacaacgc attggtcgcg ccggacatca ggttggcggt
      241 gtatctaaag ggctgttttt cccccgtacc cggcgtgatt tagtcgattc cgcagtcatt
      301 gtagagtgta tgttcgcagg caggctggaa aacctgacac caccgcataa tcctctcgac
      361 gtccttgcgc agcaaaccgt tgccgccgcg gcgatggatg cattacaggt agacgaatgg
      421 tactcccgcg tacgccgtgc cgcaccgtgg aaagatctgc caagacgtgt ttttgacgcc
      481 acgctggata tgctttccgg gcgctatccc tctggcgatt tttctgcttt tcgccccaaa
      541 ctggtctgga acagggagac cgggatattg accgcccgac ctggcgctca attgttggcg
      601 gttaccagcg gcggcaccat tccggatcgt ggcatgtata gcgtgttatt acccgaaggt
      661 gaagaaaagg ccggttcgcg gcgggtgggt gaactggatg aggagatggt atatgagtcg
      721 cgggtgaacg acattatcac tctcggcgct acctcatggc ggatccagca aatcacccgc
      781 gatcaggtga ttgtgactcc tgctccgggt cgttctgccc ggctcccctt ctggcgtggt
      841 gaaggtaacg gacgtccggc tgaattaggc gagatgatcg gcgattttct tcatttgctg
      901 gcggatggcg cgttcttttc cgggactatt cccccgtggc tggcagaaga aaatacgatc
      961 gccaatattc aggggttgat tgaggagcag cgcaacgcga cgggcatcgt tccggggagt
     1021 cgccatctgg tcctcgaacg gtgccgtgat gaaattggtg actggcgtat tattttgcac
     1081 tctccctatg gaagacgggt gcatgaaccc tgggcgctgg cgattgccgg gcgaatacat
     1141 gccctatggg gcgctgacgc gtcggtggtc gccagtgatg acggcattgt tgcacgtatt
     1201 cctgacaccg atggtaaatt gcccgatgcc gcgatttttt tgtttgaacc agaaaagttg
     1261 ctgcaaattg tccgcgaggc ggtaggcagt tcggcacttt tcgccgcccg ttttcgcgaa
     1321 tgcgccgcgc gggcattatt aatgccgggc cgcacgccgg gccatcgcac cccgctttgg
     1381 caacaacggc tgcgcgccag tcagttgctg gaaatcgctc agggatatcc ggattttccg
     1441 gtcattctcg aaaccctacg cgaatgtctg caagatgttt atgatcttcc cgcactggaa
     1501 cgtttgatgc gtcgcctgaa cggtggcgaa attcaaatat ccgatgtaac gactactacg
     1561 ccctcgcctt tcgccaccag tttattgttc ggctatgtcg cggaatttat gtaccagagc
     1621 gacgccccgc tggcagagcg ccgggcatcc gtactgtcgc tggacagcga gttactgcgc
     1681 aatctactcg gacaggtcga tccgggggaa ttactcgacc cgcaggtcat tcgccaggtg
     1741 gaagaagagt tgcaacgact ggctcctggc agaagagcga aaggtgaaga aggattgttc
     1801 gacctgctgc gcgaactggg gccaatgacc gttgaagacc tggcgcaacg gcatacaggc
     1861 agcagtgaag aggttgcgtc gtatctggaa aatcttcttg cagtaaaacg aatcttccca
     1921 gcgatgatta gcggacagga gcgtcttgcc tgtatggatg atgccgccag gctgcgtgat
     1981 gccctcggcg tacgactacc agagtcattg ccagagattt atttacatag agtcagttac
     2041 ccgcttcgcg acctctttct gcgctatctc cgggctcatg ctctggtcac ggctgaacaa
     2101 ctggctcatg agtttagtct cggtattgcc attgtcgaag agcagcttca gcaactgcgt
     2161 gaacagggtc tggtgatgaa tctgcaacaa gacatctggg tgagcgatga agtatttcgt
     2221 cgtctgcgtt tgcgctcgct gcaagccgcc agagaagcga cgcgtcccgt tgcagccacg
     2281 acctatgcgc gattgctgct ggaacgtcag ggcgtattac ccgccaccga tggtagcccg
     2341 gcgctctttg cctcaacatc gccaggcgtt tatgagggcg tagatggcgt gatgcgggtg
     2401 atcgaacagc ttgccggagt cggtttaccc gcctcactct gggaaagcca gatcctgcct
     2461 gcccgcgtac gcgactattc gtcagaaatg ctcgatgaat tactggcaac cggtgcggtt
     2521 atctggtcgg ggcaaaaaaa actgggtgaa gatgacggcc tggtggcact gcatctacag
     2581 gaatatgctg cagaatcgtt cactcccgcc gaagcggatc aggcgaatcg ttcggcgctg
     2641 cagcaagcga tagtcgctgt tctggctgac ggaggagcct ggtttgcaca acaaatcagc
     2701 caacggatac gcgacaaaat cggcgaatcg gttgatctct ctgccctgca agaggcgtta
     2761 tgggcgctgg tctggcaagg cgtcatcacc agcgacattt gggcaccgtt acgcgccctc
     2821 acccgcagca gttccaacgc acgcacctca actcgccgca gtcaccgggc tcgtcgtgga
     2881 cgtcctgtct atgcgcaacc cgtctcgccg cgggtatctt acaacacacc aaatctggct
     2941 ggacgctggt cgttattgca ggtggagcca ctaaacgata ccgaaaggat gctggcgctg
     3001 gcggaaaata tgctcgaccg ctacggcatc atcagtcgtc aggcagtgat agccgaaaat
     3061 atccctggcg ggtttccatc gatgcaaacg ctttgtcgaa gtatggaaga ctccgggcga
     3121 attatgcgag gtcgttttgt agaaggtctg ggtggcgcgc aattcgctga acgtctgact
     3181 attgaccgat tgcgcgatct ggcgacacaa gccacgcaaa cgcgccacta tacaccagtg
     3241 gcgctctctg ccaacgatcc ggctaatgtg tggggaaatc ttctgccctg gcctgcacat
     3301 ccggcaacgc tggttccaac gcgtcgggcg ggtgcgctgg tggtcgtttc tggcggcaaa
     3361 ttgttactct atctggcgca aggtggcaaa aaaatgctgg tctggcagga aaaagaggaa
     3421 ttactcgccc cagaggtttt ccacgcgctg actaccgcac tgcgtcgcga accacggctg
     3481 cgctttacgc taacagaagt gaatgatcta ccggtccggc aaacgccgat gtttacgctg
     3541 ctgcgtgagg cgggattttc aagttcgcca caagggctgg attggggata gagaaaggac
     3601 tgacggatgc ccgttcgcat ccgtcagtat tgcaggacgg attattccgc gtccggctct
     3661 tcagacttgt atttagcggc agtttctttg atcagctgct gcagttcgcc acgctgatac
     3721 atttcgatca cgatatcaca accgccgacc agctcgccgt caacccacag ttgcgggaag
     3781 gtcggccagt tagcatattt cggcagttcc gcacgaatgt ccggattctg cagaatatca
     3841 acataggcaa aacgttcgcc acatgcggca agcgcctgga ctgcctgggc agagaaaccg
     3901 cagctcggca gtttcggtga acctttcatg tacagcagga tcgggttttc agcaatctgg
     3961 cgttggattt tttcgatagt ggtgctcatt gtcttgcttc ctcaaacttc tttacggcag
     4021 taatctgaca ttgtagcggg tcagtgcggc atcggaaaat aacattttca tcacgctttt
     4081 gctattttat ccctttgctc tatttgttgc atttcaaata ttcgtttctt ctctgatgcc
     4141 ggcaaactgg cgtatttata accattttta gctttcactg ctgcattttt ttgcgctcgc
     4201 caacgaaacg tattttttaa caataaaagc tattaacttt ctcttcttct atgcattaga
     4261 atcatcaagt tttgtaaatc agacgcaggc atgatagacc tgccttaaca gagggacgct
     4321 cagtggcgcg gataaaccgt atttcgatca cgctctgtgc tttacttttt accaccctgc
     4381 ctttaacgcc tatggcccat gcttcaaagc aagccaggga gagttctgct accactcata
     4441 tcaccaaaaa agcagataaa aagaaaagca cggcaaccac caaaaagacc cagaaaacag
     4501 cgaaaaaagc cgccagtaaa agtacgacca aaagcaaaac cgcttcttcc gttaaaaaat
     4561 cttccattac cgcttctaaa aacgccaaaa ctcgcagcaa acacgccgtc aataaaacgg
     4621 cctcagccag ctttaccgaa aagtgtacca aacgtaaggg ctataaatcg cattgtgtga
     4681 aagtcaaaaa tgccgcgtca ggaactcttg ccgacgcgca caaagcgaag gtgcaaaaag
     4741 ctacgaaagt ggcaatgaat aaactgatgc agcaaattgg taagccatat cgttggggtg
     4801 gcagctcacc gcgtaccggt tttgattgca gcggcctggt ttattacgct tataaagatt
     4861 tggtgaaaat tcgtattccg cgtacggcga atgaaatgta tcacctgcgt gatgcagcgc
     4921 caatcgaacg tagtgaactg aaaaacggcg acctggtctt tttccgtact cagggacgcg
     4981 gcacagccga tcatgtcggc gtgtatgtcg gcaacggcaa atttattcag tcaccgcgca
     5041 caggtcagga aattcaaatc acttctctca gtgaagacta ctggcagcgc cactatgttg
     5101 gcgctcgtcg ggtaatgacc ccaaaaacac ttcgctaaaa ctttaccctg ttgttacggc
     5161 aacagggtaa gttcatcttt tgtctcacct tttaatttgc taccctatcc atacgcacaa
     5221 taaggctatt gtacgtatgc aaattaataa taaaggagag tagcaatgtc attcgaatta
     5281 cctgcactac catatgctaa agatgctctg gcaccgcaca tttctgcgga aactatcgag
     5341 tatcactacg gcaagcatca tcagacttat gtcactaacc tgaacaacct gattaaaggt
     5401 accgcgtttg aaggtaaatc actggaagag attattcgca gctctgaagg tggcgtattc
     5461 aacaacgcag ctcaggtctg gaaccatact ttctactgga actgcctggc accgaacgcc
     5521 ggtggcgaac cgactggaaa agtcgctgaa gctatcgccg catcttttgg cagctttgcc
     5581 gatttcaaag cgcagtttac tgatgcagcg atcaaaaact ttggttctgg ctggacctgg
     5641 ctggtgaaaa acagcgatgg caaactggct atcgtttcaa cctctaacgc gggtactccg
     5701 ctgaccaccg atgcgactcc gctgctgacc gttgatgtct gggaacacgc ttattacatc
     5761 gactatcgca atgcacgtcc tggctatctg gagcacttct gggcgctggt gaactgggaa
     5821 ttcgtagcga aaaatctcgc tgcataataa ctgatggcaa atgcagcatt gcctgatgcg
     5881 cttcgcttac catgccaacg cggatgaggc gttaacagcg cattcggcaa gataaatcgc
     5941 acgttgtcag caactgtaac gcagaaggtt atccttctgc gtttttgttt aattagctgt
     6001 tagcaacgca aactgtttca ggttgttttc tggctgacat aaacaccagc aataatgcca
     6061 gtcccgcgac aatcgctccc atcaccggca caaagctgta tcccagccca gcggaaatta
     6121 ccgcaccacc agcagctgct cccagcgcat ttccaagatt aaaggcacca atattgactg
     6181 atgaagacag acctggcgct tcactggcga cacgcatcac gcgcatctgt aacggcggta
     6241 cgaccgcaaa ggttgctgcg ccccacacca ccatgctaat agctgcgccg aactcattgc
     6301 gggccaggaa cgggattgcc agcataatca ccatcaacaa caacaaaaag cctttcaacg
     6361 tgccgttaac tgaacgatct gccagtttgc cgccgagata gttaccgata gagaatccga
     6421 caccaatcag caccagcatt gccgtgacga acaccggtgt tgcgtgggta atactttgca
     6481 gtaccggaga gatataggtg tagagagtaa acattgcacc agctcccagt accgtcgtca
     6541 gcaatgcaga cagcacctgc ggacgcatta ataccgccag ctcttttttc acttcaggtc
     6601 gtgcccctgc accaccttta ggtaatgaga agaacagact taccattgaa atcactccca
     6661 gccccgccgt tgccagaaat gacatccgcc agccgatggt ttcacccaac caggtcgccg
     6721 ccggcacgcc accgatattt gccagggtta accccataaa catagtggca actgcgctgg
     6781 cctgtttatg ttttggcacc acgcttgcgg ccacgactga acccaaacca aaaaatgctc
     6841 cgtgattcag gctggtcaaa atgcgtgaaa gcatcagggt catataatcc ggcgcgatgg
     6901 cggaaagtac gttgccgagc gtgaaaattg ccatcaggaa aatcaacgca ctgcggcggg
     6961 cacgatgaga aagtagaagc gtcatcagcg gcgcgccaac cattacgcca actgcatagg
     7021 cactgattaa cattccggca gcgggaatcg agacatccac accgcgcgca atgacgggca
     7081 acaaacccat tggcgagaac tccgttgtcc cgataccaaa cgcgccaatc gccagcgcca
     7141 gcaacggata gttaattttc atgccttatc tccacctctt cgcgtcatta cgcgatattc
     7201 attaaagtgg cgaaagcatg acagcaatca caaaaaaatg aaaataacaa aaagagaaaa
     7261 cacttttgcc attttgctaa caaacaggaa ggagatgcga gggagaacgc gctccctcga
     7321 gaggaaatca gtgcagcgcg gcagtcaaac ccacggccac gatcaaaccg aggacgataa
     7381 tcgttgttac cagtgaaaat ttaaggtcgg tgctcatcaa gttttctcct tttttattac
     7441 cacacaaata gtgatattac gcatttttac acactgtgat gaaaaaatct cccgtcattt
     7501 ataatgataa gtgtttttac cacttcccct tttcgtcaag atcggccaaa attccacgct
     7561 tacactattt gcgtactggc cattgacccc ttcctgacgc tccgtgtcgt ttttccggcg
     7621 taccgcaaca cttttgttgt gcgtaaggtg tgtaaaggca aacgtttacc ttgcgatttt
     7681 gcaggagctg aagttagggt ctggagtgaa atggaatggc aacaataaaa gatgtagcga
     7741 aacgagcaaa cgtttccact acaactgtgt cacacgtaat caacaaaaca cgtttcgtcg
     7801 ctgaagaaac gcgcaacgcc gtgtgggcag cgattaaaga attacactac tcccctagcg
     7861 cggtggcgcg tagcctgaag gttaaccaca caaagtctat cggtttgctg gcgaccagc
//
LOCUS       DAMZET010000185         7825 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.185, whole genome shotgun sequence.
ACCESSION   DAMZET010000185 DAMZET010000000
VERSION     DAMZET010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.185"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..157
                     /locus_tag="POB34_004073"
     CDS             <1..157
                     /locus_tag="POB34_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDI5920929.1"
                     /translation="KLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSVNPGLVRASA
                     TFTLEFQ"
     gene            170..673
                     /locus_tag="POB34_004074"
     CDS             170..673
                     /locus_tag="POB34_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDI5920930.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            733..1647
                     /locus_tag="POB34_004075"
     CDS             733..1647
                     /locus_tag="POB34_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI5920931.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1981..4260
                     /gene="ydeP"
                     /locus_tag="POB34_004076"
     CDS             1981..4260
                     /gene="ydeP"
                     /locus_tag="POB34_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HDI5920932.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKNGAQAVTVEDSMSMIHASRGMLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            4508..4705
                     /gene="safA"
                     /locus_tag="POB34_004077"
     CDS             4508..4705
                     /gene="safA"
                     /locus_tag="POB34_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HDI5920933.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            4780..5541
                     /gene="ydeO"
                     /locus_tag="POB34_004078"
     CDS             4780..5541
                     /gene="ydeO"
                     /locus_tag="POB34_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HDI5920934.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHTGMNTGNTMSALAI"
     gene            5943..7625
                     /locus_tag="POB34_004079"
     CDS             5943..7625
                     /locus_tag="POB34_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HDI5920935.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            7677..>7825
                     /locus_tag="POB34_004080"
     CDS             7677..>7825
                     /locus_tag="POB34_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDI5920936.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMNDSTLKEFIK
                     QYIAAS"
BASE COUNT         2280 a         1705 c         1707 g         2133 t
ORIGIN      
        1 gaaactgaat gaccttcatg ccgggatgca gtggatccca ctggtaccag aacagaacaa
       61 tattttgcct tactccgctc gtctgaagtc aactcagaag tccgtcaatc cgggactggt
      121 gagggcttcg gcaaccttta cccttgaatt tcaataatca gagtcagata tgaaaagact
      181 tcacaagagg ttcctgttag ctacgttttg cgcgttattc acagcaactc tccaggccgc
      241 cgatgtcact atcactgtta atggtcgggt agtcgctaaa ccctgcacta ttcaaaccaa
      301 agaagctaac gttaatctcg gggatcttta tacgcgcaat ctgcaacaac ctggttctgc
      361 atctggctgg cacaatatta ctttgtcatt aaccgattgt ccggttgaaa caagtgcagt
      421 gacggcaatc gtgacaggtt caactgacaa tacgggttat tacaaaaatg aaggtactgc
      481 cgaaaatatt cagatagagc tgagggatga ccaggatgcg acgttaaaaa atggcgatag
      541 caaaacggtt attgttgatg agatcactcg taatgcacag tttccactta aggcaagagc
      601 tatcacggtg aatggaaacg caagccaggg aacgatcgag gcgctaatca atgtgatcta
      661 cacctggcaa taacgtcgat tcgattttct tagccttctt tttaaatcaa ttttttaacc
      721 ttaggatata tcatgggtaa aacaatcagt ataaaggtcc tgttcggtat ttatcttcta
      781 ttaatggcag gtaaagtttt tgccttcagt tgtaatgtcg atggcgggtc aagtattgga
      841 gctggtacaa cctctgttta tgttaatctc gaccctgtaa tacagccggg ccagaatctg
      901 gttgtagact tgtctcagca tataagttgc tggaatgatt acggcggctg gtacgacact
      961 gatcatataa acctggtaca aggctcagct ttcgcaggct ctctacagtc atataaagga
     1021 tcgctgtact ggaataatgt gacctacccc ttcccgttga cgacaaatac caacgtattg
     1081 gatattggtg ataagacacc gatgccatta ccgttaaaac tctatattac cccagtcggg
     1141 gcggccggtg gagttgtcat aaaagccggg gaagttattg cccgtattca tatgtataaa
     1201 attgcaacat taggaagcgg aaatccgcgt aattttacgt ggaatattat ttcaaataac
     1261 agtgttgtta tgccaacagg cggctgcact gttgatagtc gtaatgtcac cgtggatctc
     1321 ccggactttc ctggaagcgc agaaataccc cttggcgtat attgctcaag cgaacaaaag
     1381 ctctcatttt atctttcggg tgccaccact gacagtgccc gacaagtgtt tgctaacaca
     1441 gcacctgatg caacaaaagc atcgggagtg ggtgtctctc tgatgcgtaa tggaaaaata
     1501 ctggctacgg gagaaaatgt ctccctggga acagtaaata aaagcaaagt acctctgggt
     1561 ttaagcgcaa cctatggaca aacaggaaac aaggtttcag ccggaaccgt ccagtcagtc
     1621 attggtgtca cttttatcta tgaataattg tgattttgat cttagtctat gcgctccatt
     1681 cacgaaaatt gcgcggataa ttattttgta aaggctatta tcctacacct gtaagaaaat
     1741 ccgcgcacaa ttgaagccag agtcttattt tttatattga aaaatatgtt aatttaaaca
     1801 ctatgagaga tgatgcttaa cttatcgtat ttaatctatt gtttaacgat aatagattaa
     1861 aaatagaaag agagtagtcc cctactctta tatatccatg ttggcgataa tccctttgta
     1921 tatactgtgc atcatcgcta ttacaaatcc taataattca tttccacaca ggataagtag
     1981 atgaagaaaa aaattgaatc ctaccagggt gctgccggtg gttggggtgc tgttaaatcc
     2041 gtagcgaatg cagtacgtaa gcagatggat atacgccagg atgttattgc catgtttgac
     2101 atgaataagc cagagggctt tgactgtccg ggttgtgcat ggccagatcc taagcacagt
     2161 gcgtcattcg acatttgtga aaacggcgca aaagcaatcg cctgggaagt cacggataag
     2221 caggtaaatg cctctttctt tgctgagaat acggttcaat cattacttac ctggggagat
     2281 cacgagcttg aggctgcggg acgtctcact cagcctttga aatatgatgc cgtcagcgac
     2341 tgttacaagc cattaagctg gcaacaagct ttcgacgaaa ttggcgcacg ccttcaaagc
     2401 tatagtgatc ccaatcaggt tgaattctat acttcgggcc gcacttccaa tgaagctgcc
     2461 tttctttatc agctttttgc ccgtgaatac gggagcaata actttcccga ctgctccaac
     2521 atgtgccatg aaccgacaag cgtgggtttg gcagcgagta tcggtgtagg taaagggacc
     2581 gtgttgctgg aagactttga gaagtgcgat ttagtcattt gcattgggca taaccctggt
     2641 acaaaccacc ctcgcatgct gacttcgttg cgcgctttag tgaaacgggg agcgaaaatg
     2701 atcgccatca atcctctaca ggaacgtggc ctggagcgat ttaccgcacc gcaaaacccg
     2761 tttgaaatgc tgacgaactc tgagactcag ttggccagtg cctactataa cgtgcgcatt
     2821 ggtggcgata tggcgttgct caaggggatg atgcgcctgt taattgagcg cgatgatgct
     2881 gcaagcgccg caggtcggcc ttcattgctt gatgacgagt ttattcaaac gcataccgtc
     2941 ggctttgacg agctacgccg tgacgttctc aattccgagt ggaaagatat cgaacgtatt
     3001 tctggactaa gtcagacaca aatcgccgaa ctggctgacg catatgccgc tgccgaacgc
     3061 accattatct gttacggaat ggggatcact cagcacgaac atggtaccca gaacgtacag
     3121 caactggtca atctgctgtt gatgaaaggt aacattggca agcctggtgc gggtatctgc
     3181 ccactacgtg gacactctaa tgtacagggc gaccgaaccg tcggtatcac cgagaaaccg
     3241 tctgcagagt ttctggctcg tctgggtgag cgctatggct tcaccccacc tcatgcacct
     3301 ggacatgctg caattgccag catgcaagca atatgtacgg ggcaggctcg agcattgatc
     3361 tgcatggggg gcaattttgc gctggcaatg ccagatcggg aagcgagcgc tgtaccgtta
     3421 acgcaattag atttggcggt acacgtagcc actaagctta accgctctca tctgttgacc
     3481 gcacggcata gctatattct gccggttctg ggacgtagcg agattgacat gcaaaaaaac
     3541 ggtgcgcagg cggtaaccgt tgaggattca atgtcgatga ttcatgcctc gcgtggcatg
     3601 ttaaaacccg ccggtgtaat gctgaaatca gagtgtgcag tggtcgcggg aatcgcgcag
     3661 gcagcactac cccagagcgt ggtagcctgg gagtatctgg tggaagatta tgatcgcatt
     3721 cgcaatgaca ttgaagctgt gctgccagag ttcgccgact ataaccaacg catccgtcat
     3781 cccggtggtt ttcacctgat aaatgcagct gctgaaaggc gctggatgac gccgtcaggt
     3841 aaggctaatt tcattaccag caaagggctg ttagaagatc cctcttcagc gtttaacagt
     3901 aagctggtca tggcgacagt acgcagtcac gatcaataca acacgacgat ttatggtatg
     3961 gatgatcgct atcgaggggt attcggtcaa cgagatgtgg tctttatgag tgctaaacaa
     4021 gctaaaattt gccgtgtaaa aaacggcgaa agagttaatc ttattgcgct tacgccagac
     4081 ggtaagcgca gctcacgccg catggataga ttaaaagtgg tcatttaccc tatggctgac
     4141 cgctcactgg tgacctattt tccagaatcg aatcacatgc taactcttga taaccacgat
     4201 ccattaagtg gcattcctgg ttataaaagt attccggttg aattagaacc atcaaattaa
     4261 ttcgcctcct catttcttct gctgtcatcc gcacagcaga agaatttctc attgactatt
     4321 atcgcgcaat ttgctcacat tgattaaatt aaaccacaca ctataagata taaacctctg
     4381 cctacagctg taagaaactc cgctcagtac tgaagtgcca gtcctatttc ctcttttctc
     4441 tagcctgtta tattaagcat actgattaac gatttttaac gttatccgct aaataaacat
     4501 atttgaaatg catgcgacta cagtgaaaaa caaaatcacg caaagagaca actataaaga
     4561 aatcatgtct gtaattgtgg ttgtcttatt actgaccctt acgttgatag ccattttttc
     4621 ggcaattgat cagctgggta tttcagaaat gggtcgcatt gcaagagatc ttacacattt
     4681 cattatcaat agtttgcaag actgaaaata aacagcaaat tataaatatg aaatgttaaa
     4741 aaagtatcga taaaaacttt attgttttaa ggagataaaa tgtcgctcgt ttgttctgtt
     4801 atatttattc atcatgcctt caacgctaac attttagata aagattacgc cttctctgac
     4861 ggcgagatcc tgatggtaga taacgctgtt cgtacgcatt ttgaacctta tgagcggcat
     4921 tttaaagaaa tcggatttaa tgaaaatacc attaaaaaat atctacaatg cactaacatc
     4981 cagacagtga cggtgcctgt tcctgcgaag tttttacgtg cttcaaatgt accgactgga
     5041 ttgcttaatg aaatgattgc ttatctcaac tcggaagaac gcaatcatca taatttttca
     5101 gaacttttgc ttttttcttg cctgtctatt tttgccgcat gcaaaggttt cattacacta
     5161 ttaactaacg gtgtgctatc cgtttccgga aaagtgagaa atattgtcaa catgaagctg
     5221 gcgcacccat ggaagctgaa agatatttgt gactgcctgt acatcagtga aagcctgttg
     5281 aagaaaaaac taaagcaaga gcaaacgaca ttctcacaga ttctattaga tgccagaatg
     5341 cagcacgcaa aaaatttgat acgcgtagaa ggttcagtca ataaaattgc cgaacaatgt
     5401 ggttatgcca gtacatctta ttttatttat gcgttccgca aacatttcgg caacagtccg
     5461 aagagagttt ctaaggagta ccgttgtcaa agtcacacgg gtatgaatac gggcaacacg
     5521 atgagtgctt tagctatttg attatttgct aacgagtagt caaccacgca cgctacgtaa
     5581 gaattatatg gggcagccat tccctgcccc gcgttgtttt taggcgatat atttattgaa
     5641 ataaataagt gacatccatc acatatttat gcacttgcat aacctgttgc atgattattt
     5701 atgatctcaa ttcggcattt tgtcagtaaa atgcaataat ttattaaata tcaataaatt
     5761 agttgtttat cgtcgagaaa ttacttaata gaacagaaag taatgtcaac gctttatgga
     5821 ctgttatttc ccttttttta gctaaatctg ctatctcttt atgtgactaa cttcacttac
     5881 acccacttat ttctcttcgt aaaattactt cggaattaag tacaataaga agaggaacat
     5941 ttatgaagtc tgcattaaag aaaagtgtcg taagtacctc gatatctttg atactggcat
     6001 ctggtatggc tgcatttgct gctcatgcgg cagatgatgt aaagctgaaa gcaaccaaaa
     6061 caaacgttgc tttctcagac tttacgccga ctgaatacag taccaaagga aagccaaata
     6121 ttatcgtact aaccatggat gatcttggtt atggacaact tccttttgat aagggatctt
     6181 ttgacccaaa aacaatggaa aatcgtgaag ttgtcgatac ctacaaaata gggatagata
     6241 aagccattga agctgcacaa aaatcaacgc cgacgctcct ttcattaatg gatgaaggcg
     6301 tacggtttac taacggctat gtggcacacg gtgtttccgg cccctcccgt gccgcaataa
     6361 tgaccggtcg agctcccgcc cgctttggtg tctattccaa taccgatgct caggatggta
     6421 ttccgctaac agaaactttc ttgcctgaat tattccagaa tcatggttat tacactgcag
     6481 cagtaggtaa atggcacttg tcaaaaatca gtaatgtgcc ggtaccggaa gataaacaaa
     6541 cgcgtgacta tcatgacaac ttcaccacat tttctgcgga agaatggcaa ccgcaaaacc
     6601 gtggctttga ttactttatg ggattccacg ctgcaggaac ggcatattac aactcccctt
     6661 cactgttcaa aaatcgtgaa cgtgtccccg caaaaggtta tatcagcgat cagttaaccg
     6721 atgaggcaat tggcgttgtt gatcgtgcca aaacacttga ccagcctttt atgctttacc
     6781 tggcttataa tgctccgcac ctgccaaatg ataatcctgc accggatcaa tatcagaagc
     6841 aatttaatac cggtagtcaa acagcagata actactacgc ttccgtttat tctgttgatc
     6901 agggtgtaaa acgcattctc gaacaactga agaaaaacgg acagtatgac aatacaatta
     6961 ttctctttac ctccgataat ggtgcggtta tcgatggtcc tctgccgctg aacggggcgc
     7021 aaaaaggcta taagagtcag acctatcctg gcggtactca caccccaatg tttatgtggt
     7081 ggaaaggaaa acttcaaccc ggtaattatg acaagctgat ttccgcaatg gatttctacc
     7141 cgacagctct tgatgcagcc gatatcagca ttccaaaaga ccttaagctg gatggcgttt
     7201 ccttgctgcc ctggttgcaa gataagaaac aaggcgagcc acataaaaat ctgacctgga
     7261 taacctctta ttctcactgg tttgacgagg aaaatattcc attctgggat aattaccaca
     7321 aatttgttcg ccatcagtca gacgattacc cgcataaccc caacactgag gacttaagcc
     7381 aattctctta tacggtgaga aataacgatt attcgcttgt ctatacagta gaaaacaatc
     7441 agttaggtct ctacaaactg acggatctac agcaaaaaga taaccttgcc gccgccaatc
     7501 cgcaggtcgt taaagagatg caaggcgtgg taagagagtt tatcgacagc agccagccac
     7561 cgcttagcga ggtaaatcag gagaagttta acaatatcaa gaaagcacta agcgaagcga
     7621 aataactaaa ccttcatgcg gcggattttt ccgccgcctt attgagcgag atagcgatgc
     7681 acgttacagc caagccctcc agttttcaat gtaatctcaa atgtgattac tgtttttacc
     7741 ttgaaaaaga gtcgcagttt actcatgaaa aatggatgaa tgacagcact ctgaaagagt
     7801 tcatcaaaca atatatcgca gcgtc
//
LOCUS       DAMZET010000186         7605 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.186, whole genome shotgun sequence.
ACCESSION   DAMZET010000186 DAMZET010000000
VERSION     DAMZET010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.186"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            298..555
                     /gene="ptsH"
                     /locus_tag="POB34_004081"
     CDS             298..555
                     /gene="ptsH"
                     /locus_tag="POB34_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HDI5920937.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            600..2327
                     /gene="ptsI"
                     /locus_tag="POB34_004082"
     CDS             600..2327
                     /gene="ptsI"
                     /locus_tag="POB34_004082"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HDI5920938.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            2368..2877
                     /gene="crr"
                     /locus_tag="POB34_004083"
     CDS             2368..2877
                     /gene="crr"
                     /locus_tag="POB34_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDI5920939.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(2919..3770)
                     /gene="pdxK"
                     /locus_tag="POB34_004084"
                     /gene_synonym="thiJ"
     CDS             complement(2919..3770)
                     /gene="pdxK"
                     /locus_tag="POB34_004084"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HDI5920940.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            3875..4243
                     /locus_tag="POB34_004085"
     CDS             3875..4243
                     /locus_tag="POB34_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HDI5920941.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(4246..5157)
                     /gene="cysM"
                     /locus_tag="POB34_004086"
     CDS             complement(4246..5157)
                     /gene="cysM"
                     /locus_tag="POB34_004086"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HDI5920942.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(5291..6388)
                     /gene="cysA"
                     /locus_tag="POB34_004087"
     CDS             complement(5291..6388)
                     /gene="cysA"
                     /locus_tag="POB34_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HDI5920943.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(6378..7253)
                     /gene="cysW"
                     /locus_tag="POB34_004088"
     CDS             complement(6378..7253)
                     /gene="cysW"
                     /locus_tag="POB34_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HDI5920944.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(7253..>7605)
                     /locus_tag="POB34_004089"
     CDS             complement(7253..>7605)
                     /locus_tag="POB34_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDI5920945.1"
                     /translation="VLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSF
                     TRSLGEFGAVIFIAGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLILLFSI
                     NTLQSRFGRRVVGH"
BASE COUNT         1899 a         2088 c         1894 g         1724 t
ORIGIN      
        1 attcagcctg tcggaactgg tatttaacca gactaattat tttgatgcgc gaaattaatc
       61 gttacaggaa aagccaaagc tgaatcgatt ttatgatttg gttcaattct tcctttagcg
      121 gcataatgtt taatgacgta cgaaacgtca gcggtcaaca cccgccagca atggactgta
      181 ttgcgctctt cgtgcgtcgc gtctgttaaa aactggcgct aacaatacag gctaaagtcg
      241 aaccgccagg ctagacttta gttccacaac actaaaccta taagttgggg aaatacaatg
      301 ttccagcaag aagttaccat taccgctccg aacggtctgc acacccgccc tgctgcccag
      361 tttgtaaaag aagctaaagg cttcacttct gaaattactg tgacttccaa cggcaaaagc
      421 gccagcgcga aaagcctgtt taaactgcag actctgggcc tgactcaagg taccgttgtg
      481 actatctccg cagaaggcga agacgagcag aaagcggttg aacatctggt taaactgatg
      541 gcggaactcg agtaatttcc cgggttcttt taaaaatcag tcacaagtaa ggtagggtta
      601 tgatttcagg cattttagca tccccgggta tcgctttcgg taaagctctg cttctgaaag
      661 aagacgaaat tgtcattgac cggaaaaaaa tttctgccga ccaggttgat caggaagttg
      721 aacgttttct gagcggtcgt gccaaggcat cagcccagct ggaaacgatc aaaacgaaag
      781 ctggtgaaac gttcggtgaa gaaaaagaag ccatctttga agggcatatt atgctgctcg
      841 aagatgagga gctggagcag gaaatcatag ccctgattaa agataagcac atgacagctg
      901 acgcagctgc tcatgaagtt atcgaaggtc aggcttctgc cctggaagag ctggatgatg
      961 aatacctgaa agaacgtgcg gctgacgtac gtgatatcgg taagcgcctg ctgcgcaaca
     1021 tcctgggcct gaagattatc gacctgagcg ccattcagga tgaagtcatt ctggttgccg
     1081 ctgacctgac gccgtccgaa accgcacagc tgaacctgaa gaaggtgctg ggtttcatca
     1141 ccgacgcggg tggccgtact tcccacacct ctatcatggc gcgttctctg gaactgcctg
     1201 ctatcgtggg taccggtagc gtcacctctc aggtgaaaaa tgacgactat ctgattctgg
     1261 atgccgtaaa taatcaggtt tacgtcaatc caaccaacga agttattgat aaaatgcgcg
     1321 ctgttcagga gcaagtggct tctgaaaaag cagagcttgc taaactgaaa gatctgccag
     1381 ctattacgct ggacggtcac caggtagaag tatgcgctaa cattggtacg gttcgtgacg
     1441 ttgaaggtgc agagcgtaac ggcgctgaag gcgttggtct gtatcgtact gagttcctgt
     1501 tcatggaccg cgacgcgctg cccactgaag aagaacagtt tgctgcttac aaagcagtgg
     1561 ctgaagcgtg tggctcgcaa gcggttatcg ttcgtaccat ggacatcggc ggcgacaaag
     1621 agctgccata catgaacttc ccgaaagaag agaacccgtt cctcggctgg cgcgctatcc
     1681 gtatcgcgat ggatcgtaaa gagatcctgc gcgatcagct ccgcgctatc ctgcgtgcct
     1741 cggctttcgg taaattgcgc attatgttcc cgatgatcat ctctgttgaa gaagtgcgtg
     1801 cactgcgcaa agagatcgaa atctacaaac aggaactgcg cgacgaaggt aaagcgtttg
     1861 acgagtcaat tgaaatcggc gtaatggtgg aaacaccggc tgccgcaaca attgcacgtc
     1921 atttagccaa agaagttgat ttctttagta tcggcaccaa tgatttaacg cagtacactc
     1981 tggcagttga ccgtggtaat gatatgattt cacaccttta ccaaccaatg tcaccgtccg
     2041 tgctgaactt gatcaagcaa gttattgatg cttctcatgc tgaaggcaaa tggactggca
     2101 tgtgtggtga gcttgctggc gatgaacgtg ctacacttct gttgctgggg atgggtctgg
     2161 acgaattctc tatgagcgcc atttctatcc cgcgcattaa gaagattatc cgtaacacga
     2221 acttcgaaga tgcgaaggtg ttagcagagc aggctcttgc tcaaccgaca acggacgagt
     2281 taatgacgct ggttaacaag ttcattgaag aaaaaacaat ctgctaatcc acgagatgcg
     2341 gcccaattta ctgcttagga gaagatcatg ggtttgttcg ataaactgaa atctctggtt
     2401 tccgacgaca agaaggatac cggaactatt gagatcattg ctccgctctc tggcgagatc
     2461 gtcaatatcg aagacgtgcc ggatgtcgtt tttgcggaaa aaatcgttgg tgatggtatt
     2521 gctatcaaac caacgggtaa caaaatggtc gcgcctgtag acggtaccat tggtaaaatc
     2581 tttgaaacca atcacgcgtt ctctatcgaa tctgatagcg gcgttgaact gttcgtccac
     2641 ttcggtatcg acaccgttga actgaaaggc gaaggcttca agcgtattgc tgaagaaggt
     2701 cagcgcgtga aagttggcga tactgtcatt gaatttgatc tgccgctact ggaagagaaa
     2761 gccaagtcta ccctgactcc ggttgttatc tccaacatgg acgaaatcaa agaactgatc
     2821 aaactgtccg gtagcgtaac cgtgggtgaa accccggtta tccgcatcaa gaagtaattc
     2881 ttgccgcagt gaaaaatggc gcccatcggc gccatttttt atgcttccgc cagcggcggc
     2941 aaaatcaatt catcgctctc atgctgctgg gtgtagcgca tcacttccag tacgcgcaac
     3001 cccgcacggt gcaccgcatc gtttaacgcc ttccctttca gcaaaccact gataagctga
     3061 gcacaaaaca ggtcgccagt ccctttcagg tcggttttta cccgtgaatg ggaaatgaca
     3121 ttcacgctgt cggcactgac caccaccacc tgcatctcct gattttcttc attaccggag
     3181 gcgctggtaa tcaccaccca ttttaatgtg tctgaaagca gactttttgc ggcagcaatg
     3241 gcactgtcga gatcgcggca atttttaccg gtcaggattt ccagctcaaa gatattgggg
     3301 gtaattccct gcgccagcgg cagtaaatat tgtcgatacg cctcgggaag gtcaggtttg
     3361 acataaattc cgctatcaat atcgccaatc accggatcga ccatgatcaa taggtcagga
     3421 tggtctttgc gtagcgcagt cagccactcg gcaaggattt tgatttgcga tgccgttccc
     3481 atatagcccg tggttacagc gcgcagctgg cgcagcgcat cacgctcctg aagcgcacgc
     3541 aaataaccgc taaaccattc atccggaatc gcaccaccgt agaaagtgtc ataatgcggc
     3601 gtattgctca gcaataccgt cggcacggca aaaacattca gaccgttctg tttgatagca
     3661 ggcacggcaa tgctgttgcc cacgctgccg taaaccacct gcgactgcac ggcgacgata
     3721 tccgcctgca gcgccctgct cttatcgtta aacaacaaca aactactcat ttaatttttt
     3781 ctccttgccg atgatcctca tcgtattcca accgaaactt tacctgattc tggcagtcaa
     3841 atcggctatc acaaaacaag gataaggtaa ttcaatgaag aaaatcattt gtctggtcat
     3901 tacactacta atgacactcc ccgcttacgc gaagttaact gcccatgaag aagcccgcat
     3961 caacgccatg ctggagggat tagcacagaa aaaggatttg atatttgtgc gcaacggtga
     4021 tgaacatacc tgcgatgaag cggtttctca tctgcgtctg aagctgggca atacccgtaa
     4081 ccgcattgac actgccgagc agtttattga taaggttgct tcgtcgtcat cgattaccgg
     4141 gaagccgtat attgtgaaga tccccggtaa gagcgatgag aacgcacagc cttttttaca
     4201 tgcgctaatt gcgcagacgg ataaaacggt gcctgcgcaa taatcttaaa tccccgcccc
     4261 ctggctaaaa tgctcttccc caaacacccc ggtggaaagg tagcgatcgc cacgatcgca
     4321 gatgatcgct accaccaccg caccagggtt agcttttgcc acccgcagtg ctccggcaac
     4381 cgcgccgccg gagctgacgc cacagaatat tccttcccgc actgccagtt cgcgcatggt
     4441 gttttctgca tcgcgctgat gaatatccag cacctcatcc accagagaag cgttgaaaat
     4501 ccccggcaga tattccgcag gccagcggcg aatgccggga atactgctgc cttcttccgg
     4561 ctgcaggcca acaatcgtta ctggtttgga ttgttcgcgc ataaagcgtg aaacgccggt
     4621 gatagtgccg gttgttccca tgctggagac aaaatgggta atgcgcccgc cggtttgctg
     4681 ccagatttcc ggcccggtag tggtgtaatg ggcgtaaggg ttatcgggat tattaaactg
     4741 atcgagcagc tttccttcgc cacgattcgc catctccagc gccagatcgc gcgcaccttc
     4801 catgccctgc tctttggtga caagaatcag ttccgcacca taagcacgca tcgccgcacg
     4861 gcgttcctgg ctcatgttgt cgggcatcag caatttcatg cgatagcctt tcagcgcggc
     4921 aatcattgcc agcgcaatgc cggtgttacc actggtggct tcgattaaga catcacccgg
     4981 tttaatttcc ccgcgctttt ccgcctcgac gatcatcgaa agtgccgcac gatctttcac
     5041 cgaacctgcc gggttattgc cttccagttt taaccacact tcactgccgt tatccggccc
     5101 cattcgctgc aacttcacca gaggcgtatt gcctattgtt tgttctaatg tactcacgat
     5161 ctctatccat acgtggtgtt gcctgatgcg acgcttgcgc gtcttatcag gtctacaggt
     5221 tacaaacctt gccattaaaa agcccggatc gcgggaagcg cctccgggcg tttaacattc
     5281 actcaaccta tcaggcgctt tgtgcgagag caagttcctc atctcgggtt tcgatacgct
     5341 cgtcgccgtt atacagccgc gcatgttgca gaccaacgaa taaacgctcg ccacgctgcg
     5401 gggcatcgtc gccatgcatc acgaccgtca gcggttcgtt gtaccacccc agcggctgca
     5461 ccactaattg ggtgtagtga cctttcgggc tggcttccag tacctgtacc ggcagcggcg
     5521 aatcgagact ggtacggcgg ctgatatcca cttcccacgg gcgcaggaag agatccaccg
     5581 gaccctgata cgcaggtgtg tagcccagcg gccagcgatg cgcgccaaca tggaactgcc
     5641 cgccgcgaat ggttccctgc aggcggttca cttcgcccat aaattcgagt acaaaacggg
     5701 tcgctggttc gcgccatacc tgattcggcg catcagcctg ttcaatattc ccctgactca
     5761 tcaccactac acgatcggct acttcggtcg cctcttcctg gtcgtgggtc acgaacacgc
     5821 tggtgaattt cagctcttca tgcagttgac gcagccagcg acgcagctct ttacgcacct
     5881 gcgcatccag cgcgccaaac ggttcatcaa gcagcagaat ttgcggttct acagccagcg
     5941 cgcgcgccag cgccacgcgc tgtttctggc cgccggaaag ctgtgccgga taacgatctg
     6001 ccagatgagc aagctgcacc atctccagca attttgtcac tttcgctttg atggctgcag
     6061 cattcgggcg atcgcgacgc ggcagcaccg tcaggccaaa agcgatattg tcgaataccg
     6121 tcatatggcg gaacagcgcg taatgctgga acacgaaacc gaccttgcga tcgcgcgcgt
     6181 gcaggcggct gacgtcggtg ccgtggaagc gaatatgccc gctggtttga tgctccagcc
     6241 cggcgataat gcgcagcagc gtggttttcc cggaaccgga cggccccagc aacgcgacca
     6301 tctgacctga aggaatatcc agtgagatat cgttcagcac ctgggtgcga ccaaacgact
     6361 tcttaatatt ggcaatctca atgctcatga tgttcctcct gctgtgcgcg tttttcctga
     6421 ttctccaggc gccactgcaa catacttttt aaaaacaggg tgataatcgc catcagcgtt
     6481 aacagcgccg cagcggtaaa ggagccgacg gtgttgtagt cctgctccag caattcaatc
     6541 tgtaacggca gcgacagggt ttcgccgcga atcgagccgg aaaccaccga caccgcgcca
     6601 aactcgccaa ttgcgcgggc gttggtcaac accacgccat aaagcagcgc ccagcggatg
     6661 ttcggtaatg tgacgcgacg gaacatctgc cagccggacg cgccaagcaa aatcgccgct
     6721 tcgtcttcct ggctgccctg gcttaacatc accggcacca gttcgcgcac cacaaacgga
     6781 cacgtcacga agatggtgac cagcaccatt cccggccagg agaacataat ttgcaggtta
     6841 tgctcgtcga gccaaccgcc gagcgggccg ttagagccgt agaacagcaa atacaccaga
     6901 ccggcaacca ccggcgacac ggcaaacgga atgtccagca gcgtcagcag caactggcgt
     6961 ccggggaagt taaagcgcgt caccagccac gccagtagaa taccgaacac caggtttacc
     7021 ggtacggcaa tcagcgcgat catcaccgtc agccagatgg cgtgcagcat atccggatcg
     7081 gccagattct gtaaaacagg catcagccct ttgctgaacg cctgtacgaa gatataaatc
     7141 atcggcacca gcaggatgaa cgccgatacc agcatcccga tgccaatcag aaaccatttg
     7201 ccccagttaa tcgggcgcgc gtcataacgc ttcaattgtg tcacttccgc cattaatgac
     7261 ctaccacacg ccgaccaaag cgactttgca gagtgttaat tgagaacagc agtatcaggg
     7321 atgccgcgag gatcaccgaa gcaatcgcgc tcgccgccgg gtaatcaaac tcctgcaagc
     7381 gcacaaaaat catcagcgac gtcacttccg tcttccacgc gatatttccg gcgataaaaa
     7441 tcaccgcgcc aaattcacca agactacggg taaacgacag cgccacgccc gccaccagcg
     7501 ccggagaaag ctccggcagc accactttgc agaaactctg ccagcgcgtt gcaccaagcg
     7561 tttccgccgc ttcttcatat tccgggccta actcttccag caccg
//
LOCUS       DAMZET010000187         7434 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.187, whole genome shotgun sequence.
ACCESSION   DAMZET010000187 DAMZET010000000
VERSION     DAMZET010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.187"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(46..2226)
                     /gene="etk"
                     /locus_tag="POB34_004090"
     CDS             complement(46..2226)
                     /gene="etk"
                     /locus_tag="POB34_004090"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HDI5920946.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGINVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(2246..2692)
                     /gene="etp"
                     /locus_tag="POB34_004091"
                     /gene_synonym="yccY"
     CDS             complement(2246..2692)
                     /gene="etp"
                     /locus_tag="POB34_004091"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HDI5920947.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(2680..3819)
                     /locus_tag="POB34_004092"
     CDS             complement(2680..3819)
                     /locus_tag="POB34_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HDI5920948.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(3865..5961)
                     /locus_tag="POB34_004093"
     CDS             complement(3865..5961)
                     /locus_tag="POB34_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HDI5920949.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGHWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVLWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(5961..6707)
                     /locus_tag="POB34_004094"
     CDS             complement(5961..6707)
                     /locus_tag="POB34_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HDI5920950.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(6704..7348)
                     /gene="gfcB"
                     /locus_tag="POB34_004095"
     CDS             complement(6704..7348)
                     /gene="gfcB"
                     /locus_tag="POB34_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HDI5920951.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
BASE COUNT         1782 a         1960 c         1817 g         1875 t
ORIGIN      
        1 ccggaagagg cgttcacgcc gcatccggtc actttacctt cagttttact ctttctcgga
       61 gtaactataa ccgtaatagt tatagccgta actgtaagcg gtgctggcgc gtttaatcac
      121 accattgagg attgcgcctt taatattaac gccagcctgt tccagacgct gcattgacaa
      181 gctcacctct ttggcggtgt tcaagccaaa acgcgcaacc agcaggctgg tgccgacaga
      241 gcgtcccacg accgcagcat cgctcaccgc cagcatcggc ggcgtatcga caatcaccag
      301 atcgtaatgg tcgttcgccc attccagtaa ttgacgcatc cgatcgcgca tcagcagttc
      361 agacgggtta ggtggcacct gaccgcgagt aatcacatca aagcctcctt tgccaaaatg
      421 ctggatcact ttgttgagct catctttacc tgccagatat tccgacaagc catgttcatt
      481 actcacggta aacaggttat gcgaataacc acggcgtaag tcggcatcaa taaataacac
      541 tttttgatcg gactgggcga tcaccgctgc cagagttgaa ctgacaaacg ttttaccact
      601 gtctggcgtc gcaccggtga tcatcagaat gttattctcc gtctccatca tagcgaagtg
      661 cagactggtt cgtagcgcac gtacggcttc cacagcagaa tccgccgggt tatccaccgc
      721 caggaagggg atatttttag tacgatggcg ctgctgatta gaaaataaat ttttcttacg
      781 cagacgggtg cgtttatcca gccactcgga cattgggata gtggcataaa cgttgatgcc
      841 gtgctcttcc agttgttccg gtgcttctac accacgacgc aacatcgctc gcgccagcac
      901 ggcacccacc gaaataaaca ggccaagaat aaaaccaagc accacgttca acgctttttt
      961 cggtttcact ggctgcggct gagtgactgc cgggtcgata atccgcacgt taccaatggc
     1021 actggatttc gaaatactca actcctgctg gcggttaagt aattgcagat ataccgcacg
     1081 gcccgcttct acgtcacgac ttaaacgcaa cacttcctgt tgggtggaag gcatagccga
     1141 tacccgctta ttcaggcgtt tgcgttcttg ctccagcgtc tggcgttttt ccagcagcgc
     1201 acgataagtt gggtgatctt tcttatacag ctgggagatc tctgcctcgc ggaaagtcag
     1261 ctcattgagt tgattatcaa cgttcacaat ctgctcaaga acggctttag cttccaggtt
     1321 aaggtcaacc gaatcgcgct gctggcgata aacgttgagt ttttcttccg cctggtccag
     1381 ctcgctgcgc acttcaggca actggcgctg taagaattca aggctttgtg aatcctgcgc
     1441 cgcctggcga gcgatattct gttgcaaata gttgttagcg atgctgttca gaatacgagt
     1501 aatcaactgg ggatcatcac cggtcatggt aagttccagc atcccacttt ctttactgcg
     1561 ttcgctaacg gtaaaggttt cctgcaacgc gttaatcgct tccagttcgg tacgctggct
     1621 caggacaaac tgtgttcctg gtttggcctt aatatccgcg atagtcagcg caacgccatc
     1681 tttttccaga cgctggccga ccataccatt gacggtgaac tcttcacctt ccagcgtata
     1741 gtggccgttt tccccaaccg tgagtgtcag ttgctgatcc tgaccattca gttgtggaat
     1801 atgcatccag ctgatcgcca gctcacctgg tttctctttg gttaatctcg cccagccgcg
     1861 acccacaatc ggaaaatact tctgctcaac catgtcgcgc agattcagtt cagcaatggt
     1921 tttaccgaga atcatgcgcg attgcagcag ttggatctcc ggtgcagact cgggcgatga
     1981 gttagggatc atatcgctca ggccactgag aatggcgttg ccctgtttct gctcaacctg
     2041 gaccagagta tctgcctgat aaattggcgt gcttaacagc gagtaagcga cagcgatcag
     2101 cgtgaataac gcggtcacgc tgataataaa cttacggtga tcccataact cgccgaccag
     2161 acgaagcaga tcgatctcat tttcctgagt gctgcctggt ggcgtattca tatttttagt
     2221 tgtcatcgta ttttataaac tcaaattacc ggctgaggcg cttcgcccat tcctgactgg
     2281 cgcgctccaa cataccgtag acatgttcaa atgcgtcctg acttttacga tagggatccg
     2341 ggatctcttt ctgttccagc cattgcccaa acagcattgt cttcccgcgg acctcgggag
     2401 cgattgcggt aacctgagca atatgttccg actccattgc cagaatcaga tcgtaatttc
     2461 gtgccatctc agcagtgagc ttacgtccgg catgtccttc aagagatacg ccatgattag
     2521 cggcaacatc tgccgctgtc gcatctgcag ggtgttttac cagaccatga acacctgccg
     2581 atttgacttt tacgcccggc agacgtttac gcagcaagcg ctcgccaatg ggcgaacggc
     2641 aaatgttgcc ggtacaaacc accaggattg agttaaattt tagttgggcc atgtttgaat
     2701 ccaccgtgaa gtctctgtca ggtcatgcac cccggaaatc gtcggtacca gcagagaaat
     2761 cacacggttc caacgagcca gcggcgcggt agtgacataa acaatgtcgt aaggttcaag
     2821 ctggaattcc gttccgagta tcattgccga tgcgtccttc gcattcagct gataaatatt
     2881 ggcgattttg ccattctgct tgttttgcgt tgcacgaata acaaaaatcc ccgtcgcatc
     2941 agcgacatcc tgattcatac cctcagcgtt acccagcgct tccgccaggg tcataccgct
     3001 gcggtccatt ttcagcgtgc tctgtttacc cacttcaccc atcacgaaga ctttcagatc
     3061 atcgttgcgc ggaataaata aaatatcgcc cgggtgcagc agtttgttct ggcgcaagtc
     3121 gccacgctgc atcagcgcat agaggttcac tttggtcttt acgccattct gggtgagcac
     3181 cacgttgcgc cagtctgcat ctgcggtcag accgcccgcg gcgttaatcg catccatgat
     3241 ggtcagagga atattggtaa tcggctgctg acccgatttg ctgacttcgc cggtgacgta
     3301 cgctttttgc gatcggaacg ccgcgacgct gacatcgact tgcgggcttt caatgactga
     3361 atcgagacgc gctgtaatct cgttgcgtac ctgggtaagt gttttcccgg ccactttcag
     3421 gcgaccaata taggggtaga aaatcgcacc atcggcgtta acccagttgc cggtatcact
     3481 ggcgctacgg tattgccctg cgggggtggt tagttccggg tgatcccaga ccgtaaccat
     3541 caacacatca ccaatcccga tacgatattc atagtttgcg atttgctgct ccagctccgg
     3601 gttagcctgc gacataatcg gtttggcgcg caactgatca atcaatcctg gcgtgacggg
     3661 atagatattg accattttgt ccaggtcata ctgattgtca ggtagttcaa tgacatcctt
     3721 gttactggtc gataaattct gaccaggaac aagggtacat gctgtcaaag acagaacagc
     3781 caatgtcagt accgaaaact taaaaatgtt ctttttcatt ggtttatatt tatcagtcaa
     3841 tgtaatgtta tgaattacgt caggttattg tccaaccggt acttctcgct ctgccgtcat
     3901 tggatagagc tggtatttgc gtgacagcat ttgtccacca tcacgcgtca atggtgtcca
     3961 caaaaccacc gcgcggttgc ggttaggtcc aatggtcatc aagtcgaatg gaatcgagat
     4021 ataaaaacct ttactaaagc cgccttcgcc gtagtcatct ttagatacgt tactgattgc
     4081 cgcccatacc cctaccgcca cgccgctgtc aaagcgtttg gcgacgtcga tcgttgcgcc
     4141 tttatctttt gccagatatt gcccaacgct aagtttcatc agtacgccat tgagcgtcgg
     4201 cgggttccag taagccgtca cgaaacctgt tggcgtggaa taatcggtga aacgcatcat
     4261 gttgtcccag tcacgctgct tcacgtagtt aacgtccaca cccagcgccc agctggcatc
     4321 tagcgggcga taaagcagct cggaaccgac accggcatac atcgtttcca gataaccgcc
     4381 atacacctgg ccatagaaac cattgcctaa gtcagcaaaa tagttcgcct gcaagttgtt
     4441 gagataaacg tcattgcgaa cgtagtcacg gatatgcgtg cgcacgcggg gcagggtaga
     4501 gtccgcgggc aacagcgaag acttaaactt gtcgtagttg ttgtaaatat tggtgaaaat
     4561 accgccatca agcagcaggt ggtcggtaaa ccagtagcgg gcactggaca tcagccccag
     4621 ctggaacata tagaaatctt ccgggccgcc cagcgactgt gaaagtgttg ggttgaaaga
     4681 gtagctaaag cgatcttcac gaatacggta gccccgacca aaggcagaaa gatcttctgc
     4741 ttcaacacgt tgttgttgca gttgctctga ttgaccaggc gctgtaccag ccagctgttt
     4801 gcgcaggctg gctacgtcgg tttcggtagt caccatcgcc atatgctcgc gcttttgcgt
     4861 cacgctaatc ttctcaacgc cttgcggcag gttattcacc agaatccggt tggcacgatc
     4921 gaccgcttca cgagagtcac ggtatttgta ttgctgacca gacatataca gcgtcttatc
     4981 gcgtagctga atttctggcg cgtcaaagcc cgcgttatac ttcagggcgg taagttggtt
     5041 tgctaccgtg gtgtactgca atccttcaga ttcaggcgca ggttgatatg ccggttttgg
     5101 cgtatcgcgc agggcagggc gcagatcgtt gaaattggtc cgtaaagtga aaccaaacat
     5161 caacgtgtta ccgcgttcat aactcaggtt gagatctgcc cagctggcag cgcgataaac
     5221 tgcaccgacg ttgaaatggc ttgcctgagg cagtttgcca gcgaaatcat tctggtaatt
     5281 gttgccgtcg tattcgagtt tcagacgcag gggattccac ggcgtttgat actcaatgcc
     5341 gccaaagatg gaagccgggc cacgaaagat atcgctaaag ctgatatcgc ccgcatcgtg
     5401 agactctgcg cgatgacaat atttatcgct cacccggcaa aacgggttgg taatattgcc
     5461 cgcattaccg gcgtagcccc atgccatccc gagggtgaaa tcaaatggcc ccgcttgctt
     5521 gctggccacc agatactcac cgtcaaacag acccgtacca gcaatatcac gtttaccaaa
     5581 cgccacttgc ggtagccagt gaccttcttc ccacaggcga agtttaaaat cgaatgattt
     5641 gtctttgtat gactgatcgc cgctgaaatc ttcccactgg ctatatttgc gtgtgcgcac
     5701 atccgtataa cgaatggtgc cttccagcca tgggaacagc gccacggagg tggagtagaa
     5761 ccggtattga tcgttatccc ggtagttgac gctgaattca ccttccggtg cgatgcgcgc
     5821 atttggcatc tgcaacaatc ctgtgccgcc aaagtctgat tgcgacgaac ccagcggatc
     5881 cgggtaggtt aatacttcag catgacaggc actggagacg gcaatggcca ggcagcttaa
     5941 aagataagaa ttcttcttca tcagtcagga acacgttgcg ttaaaacaga cacaatctgg
     6001 tcatttaaat cagcatattt ttcgggcagt acatgcgcag aaaagcccaa ccaaagctgg
     6061 ctgccaggcg gtggttccac atgtctttta ttccacaggg caacaggggc gacgacagtc
     6121 tcaccctcag gcgtaatcac cataacgtta ttcttgtccg cgcctgcaag gcgggggtga
     6181 tcttgcagat aatcggtaac gctacggcct gcctgccagg gcaattgccc ggccccagaa
     6241 accgcaccca gcagagtaat agtcaccgga cgctgtacgg tatagagcgt atagtccccg
     6301 accaacgggg gattgctgtt ctcatccact cgtacaaaat cagggtccag tttgaccggt
     6361 aagcgaccag tgatattgag attgagtaac tgctggcgca cggatttaat cgttgccgcc
     6421 acatcgtcgt cagcttctgc ttcccaggaa gcaagctgtg ccatgacatg ttggtagtct
     6481 ttaagcgctt tagctttcgc cgcagagtcg gtcagcaagg caccaggcca ccagaggcga
     6541 tcgcgcagtt gcggttgtgt caccaactgg acaacatttt ccacggggcc aacggaaagt
     6601 gtctgttgtt cgccaggcag ataaatagtc accgttcctt gcgcaaaggc atggggtgtc
     6661 attacgtaaa gtacgctggc aatgaaatac gactgtaatt tattcatggt gcgggtttaa
     6721 gaaacgtcat ttctacggga ataacccctg cgcctaacat ttgccgactt tgacgaacct
     6781 ggccagtagc cgaatcgacc cagaatgtgt tttgccactg tttttctggc cgcgtggaag
     6841 tcacctcttc ctgccagaca ttacacagca cattgcgacc cgcaatattc agcgtctcta
     6901 atccagcaaa ggaaaaagta gaactcaggg tcgcagagcg aaaatggttg tcttcactcc
     6961 agagaatatc gcgcgtccag cggctgccat cttttattgc cagcgcgtta cgcagagggt
     7021 cctgcccgga atttgtcact tccagcagat tattattaag tttgacggtt tttaaaagac
     7081 gtccattgtg ggtaaccagc atggcgttat cctgggataa ccatttgctt tgttcttgtt
     7141 ctatatatcc cagtaccaca aagattcgtt gcccttcatt aaggcgtaaa tacatcgtgg
     7201 aataaggcaa cgcctggatc tgctgatccg ctacggtgat atcctgatta tcgaaaaggc
     7261 tggcgcgaaa tgtatcgacc atactttgct ggctgtgcgt acatcccgca agaaatagtg
     7321 cgaaaatcga tagaataaga gggcgcacct ttaatccttg aaaggttaat taaaaaaatg
     7381 caaatctcta aagcccggta cgaatatcgc aaccgggtat catttaactt taat
//
LOCUS       DAMZET010000188         7421 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.188, whole genome shotgun sequence.
ACCESSION   DAMZET010000188 DAMZET010000000
VERSION     DAMZET010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.188"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..322)
                     /gene="rclR"
                     /locus_tag="POB34_004096"
     CDS             complement(<1..322)
                     /gene="rclR"
                     /locus_tag="POB34_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HDI5920952.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPE"
     gene            548..1873
                     /locus_tag="POB34_004097"
     CDS             548..1873
                     /locus_tag="POB34_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HDI5920953.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREERDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            1982..2218
                     /locus_tag="POB34_004098"
     CDS             1982..2218
                     /locus_tag="POB34_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDI5920954.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKTILKHISVSSVSASPTVLE
                     DAIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            2230..2823
                     /gene="rclC"
                     /locus_tag="POB34_004099"
     CDS             2230..2823
                     /gene="rclC"
                     /locus_tag="POB34_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HDI5920955.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(2983..3111)
                     /locus_tag="POB34_004100"
                     /pseudo
     CDS             complement(2983..3111)
                     /locus_tag="POB34_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            3414..4265
                     /locus_tag="POB34_004101"
     CDS             3414..4265
                     /locus_tag="POB34_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5920956.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLINFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCEYADMPKEWTTTRGLY
                     ASFRYEGNWENYPDWVRNIYLIELPARGLARVNGSDIERFYYNEDFVEKDGNDVVCEI
                     FIPVRPV"
     gene            complement(4405..>7421)
                     /gene="fdeC"
                     /locus_tag="POB34_004102"
     CDS             complement(4405..>7421)
                     /gene="fdeC"
                     /locus_tag="POB34_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001635811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
                     /protein_id="HDI5920957.1"
                     /translation="WQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTEVVITGAGM
                     SADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTELAFKPAGNI
                     VTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMSKTVTAELR
                     ATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLRFVPQDTNG
                     VTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGPLDAAHSSI
                     TLNPDKPVVGGTVTAIWTAKDAYDNPVTSLTPEAPSLAGAAAVGSTASGWTNNDDGTW
                     TAQITLGSTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAEDHVKAGEST
                     TVTLIAKDAHGNTISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTTGGKTGELR
                     VMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKMTTELTFTVKDAYGNPV
                     TGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVATLTLGSAAGQLSVMPRVNGQNA
                     VAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPLQGQEVTLT
                     LPQGVTSKTGNTVTTNAAGKVDIELMSTVAGEHSITASVNNAQKTVTVKFKADFSTGQ
                     ATLEVDGSTPKVANDNDAFTLTATVKDQYGNLLPGAVVVFNLPRGVKPLADGNIMVNA
                     DKEGKAELKVVSVTAGTYEITASAGNDQPSNAQSVTFVADKTTATISSIEVIGNRAVA
                     DGKTKQTYKVTVTDANNNLLKDSDVTLTASSENLVLDPKGTAKTNEQGQAVFTGSTTI
                     AATYTLTAKVEQANGQVSTKTAESKFVADDKNAVLAASPERVDSLVADGKTTATMTVT
                     LMAGVNPVGGSMWVDIEAPEGVTEKDYQFLPSKADHFSGGKITRTFSTSKPGVYTFTF
                     NALTYGGYEMTPVKVTINAVAAETENGEEEMP"
BASE COUNT         1899 a         1802 c         1675 g         2045 t
ORIGIN      
        1 tttccggcaa agacgttaaa aaataacgcg ccgaatgttg cagccgaaga gtgccgcaga
       61 caatacaggt tgattcatta tcgacatgac tcaggcgatg ggctgaattt tgtggtagca
      121 ggaccacatt tcccgggcgt aatgtaaaaa tctcccccgt cggcatttcc agcttcgccg
      181 ctccttgcgt taacgcatgc caacgaataa ccgataattc cccggcacca tgtggaagct
      241 gccagtcact tcctaacacg caattcttat cgatcgttcc ttgtggagcg ttaagcatca
      301 acaaacggct aagggcatcc atatcagact cctgagtggt ttttgattac aaacaccaac
      361 aatcttagta aataactaat ctaataccat caaatagtta atcatgattt ttgttttgtg
      421 attctgatta agaaaaagaa aataccagac gataagacaa aatatcaagc gttgtgaaga
      481 aatgttattt gctcttttgc gtctatagtc atgatgtcaa atgaacgcgt ttcgacagga
      541 aatcatcatg aataaatatc aggcagtgat tattggtttt ggcaaggctg gaaaaacatt
      601 agccgtcacg ctggcaaaag caggttggcg agtggctctc atcgaacaat caaatgcaat
      661 gtatggcggg acctgtatta atatcggctg catcccaacc aaaacattgg ttcatgacgc
      721 acagcagcac acagattttg tccgtgccat acagcgtaaa aatgaagtgg ttaatttttt
      781 acgtaataag aattttcata atcttgcgga tatgcccaat atcgacgtga tcgacggcca
      841 ggcggagttt atcaataatc atagcctgcg tgttcatcgg cctgagggaa atctggaaat
      901 tcatggcgag aaaattttta ttaataccgg tgcacaaacc gtggttccgc caattcctgg
      961 aattaccacc acgccaggag tatatgacag caccggatta cttaatctaa aagaattgcc
     1021 tgggcattta ggtattttgg gcggcggata tattggcgtt gagttcgcct ctatgttcgc
     1081 taattttggc agcaaggtaa ccattttaga agcagcttca ctgtttttgc ctcgggaaga
     1141 acgggatatt gctgataata tcgcgacgat tttacgcgat cagggcgtcg atattatcct
     1201 caatgcccat gtggagcgaa tcagtcacca tgaaaatcaa gtgcaagtgc atagcgagca
     1261 cgcccaactg gcggtggatg cactgttaat agcttccggt cgtcaaccgg ctaccgcttc
     1321 gttacatcca gaaaatgccg gtatcgcagt aaacgagcgc ggggcaattg tcgttgacaa
     1381 gcgattacat accaccgcag acaatatttg ggcgatggga gatgttaccg gcgggctgca
     1441 atttacttac atatcactgg atgattaccg cattgtacgt gatgagttac tgggtgaagg
     1501 caaacgtagt actgatgatc ggaaaaatgt gccttattcc gtatttatga caccgcccct
     1561 gtccagggtt ggtatgacag aagaacaagc cagagagagt ggtgctgata ttcaggtggt
     1621 gacattgcct gtagctgcaa ttccgcgcgc cagagtgatg aacgataccc gtggggtatt
     1681 aaaagcgatt gttgataata aaacccaacg tatattaggg gcatcactgc tgtgtgttga
     1741 ctcccacgag atgattaata tagtgaaaat ggtgatggat gccgggctgc cttatagcat
     1801 attacgcgat cagatattta ctcatccgtc gatgagcgaa tcactcaatg atctattttc
     1861 attagtcaaa taaactcaaa atcagacgcc agaacaaata ttctggcgtc tcagagaaaa
     1921 gaatcttatt aattccctgt tactctataa cttcttaatc acttcattga tggtatttta
     1981 tatgtttaaa aaatccgttt tatttgcaac actattatct ggcgttatgg cattttccac
     2041 caatgcagat gataaaacaa ttctgaaaca tatcagcgtc tcgtcagtat cagcatcacc
     2101 gacagttctg gaggatgcca ttgctgatat agccagaaaa tataatgctt catcctggaa
     2161 agtcacatcg atgcgaattg ataataattc aaccgcaaca gcagtattgt ataaataagg
     2221 atgttcacaa tggaaaaata cctgcacctg ttaagtcggg gagataaaat tggcctgaca
     2281 ttgattcgtc tgagtattgc aattgttttt atgtggattg ggttattaaa gtttgtccct
     2341 tacgaggcag acagcattac accattcgtc gcaaacagtc cactaatgtc gttcttttat
     2401 gaacacccgg aagactataa acagtatctg actcacgaag gcgaatacaa accagaagca
     2461 agggcatggc aatcggccaa taatacctat ggtttttcca acggtcttgg cgtcgtggag
     2521 gtgattattg ctctgctggt tttggctaat cctgtcaatc gctggttagg tttattggga
     2581 gggctgatgg catttaccac accgttggta acactctcat ttttaatcac caccccggag
     2641 gcatgggtac ccgcattggg tgacgctcat catggtttcc cttatttatc cggtgctggt
     2701 cgcctggtat tgaaagatac tctgatgctg gcaggtgcag tcatgataat ggcagattcg
     2761 gcgcgggaaa ttcttaaaca acgcagtaat gaatccagtt caacgttaaa aactgaatat
     2821 tgataatccg aacactgtct gttgttcact ttttcaggcg tcaggccccc tttctgatga
     2881 tgaagcctga cgccatatta ccggagatat gacagagtga agatcaatat cactcccctg
     2941 ttaaaaggtt tttgtcgtct taggtaaaat acatgattaa tatcaatcat gtatttttac
     3001 agcaagacaa ccacgaacaa attcaggatt aaaatgtttt accgactccc caacataacc
     3061 caaatcaagc gaactaattt gagccatttc tttatctgtc agtgagaaat cgatttttga
     3121 tgggcatctg caatactctg aagcagacta tacggaaaat tccactgtca gtccctccat
     3181 taggcatgaa caatgagtct acgttaaaac gtaacctcaa agtagtatgt ggattttgat
     3241 atcacttatg caaaaaattc attaataatg taggactgaa acctctctat tttcggggac
     3301 aacgaagcag acgctaccag tgcttttgcc ttcgcccttg ctatttttga tacacttagg
     3361 gcccagggta taacgaaaat gtgcgatatg acaaagaatt aacggagaat gagatgatca
     3421 ggcagaagat tctacagcag ctcctggagt ggattgagtg caatcttgag caccctattt
     3481 caatcgaaga tatcgcacag aaatctggct acagcagacg caacatccag cttctgttcc
     3541 gaaatttcat gcatgtgcct ttgggagaat acattcgcaa acgaaggctt tgtcgtgccg
     3601 ccattcttgt ccggctcacc gcgaaatcta tgcttgatat tgcactctct ttgcattttg
     3661 attcacagca gtcattcagc cgcgaattta aaaagttatt cggctgctct ccccgtgaat
     3721 accgccaccg tgattattgg gatctcgcaa atatcttccc ttctttttta atacgtcaac
     3781 agcaaaagac ggagtgtaga ttaatcaact ttcctgagac acctattttt ggcaactcat
     3841 ttaaatatga cattgaagtg tcgaataaat caccggatga agaagtcaaa ctacgacgtc
     3901 atcatttagc cagatgtatg aagaatttta agacggatat ctatttcgtt tccacgtttg
     3961 aaccgtcaac aaaatcggtc gatttgctca cggttgaaac ttttgctggt acggtatgtg
     4021 aatatgctga catgccaaaa gagtggacaa cgacccgagg actttatgcc tctttccgtt
     4081 atgaaggaaa ctgggaaaat tatcctgact gggtgcgtaa catctatctg atagagttac
     4141 ctgccagggg gttagccaga gtgaacggca gcgatattga gcgcttttat tacaatgaag
     4201 atttcgtaga aaaggatggc aatgatgttg tttgcgaaat ttttattccc gttcgtccgg
     4261 tttagttggt cactatctct tattgagttt tatccttgcc tgatactatt taatcagtat
     4321 caggcaaggt attcagtgaa taatggcgtt gaatatttca acaccattat tctttattag
     4381 atcgtaactt tcatactatt caaattatgg catctcctcc tcgccatttt cagtctctgc
     4441 agcaacggcg ttaattgtca ccttcacagg cgtcatttcg tacccgccat acgtcagtgc
     4501 gttgaatgtg aacgtataga cacctggctt gctggtacta aatgtacgcg tgattttccc
     4561 acctgagaaa tggtcagcct tcgacggcag gaattgataa tccttctccg tcactccttc
     4621 cggagcctca atgtcgaccc acatacttcc tcctacggga ttgactcccg ccatcagggt
     4681 aaccgtcatt gttgcagtag tcttcccgtc cgccaccaga gaatctacac gttctggaga
     4741 tgcggcgagc accgcgtttt tatcatccgc gacgaattta gattcagcag ttttcgtcga
     4801 tacctgaccg ttggcctgtt ccactttcgc cgtgagtgta tatgtcgctg cgatagtggt
     4861 agagccggtg aaaacagcct gtccttgctc attagttttc gccgtccctt taggatccag
     4921 aactaaattt tccgagctgg cagtcagcgt cacgtcgcta tccttcaaca ggttgttatt
     4981 ggcatcagtc accgtaactt tatacgtctg tttggttttg ccatccgcca ctgcacggtt
     5041 gccaatcacc tcaatactgg agatggtcgc cgtagtctta tcggccacaa acgttacaga
     5101 ctgcgcattc gaaggctggt catttcctgc cgacgccgtg atctcatacg ttccggcagt
     5161 cacggagacc actttcagtt ccgctttacc ctccttgtcg gcgttcacca tgatattacc
     5221 gtctgcaagc ggtttgacgc cccgaggcag attaaagacg accacagcgc caggcagaag
     5281 gttgccgtat tgatccttaa ccgttgccgt cagcgtaaag gcatcattgt cgtttgccac
     5341 ttttggcgtg ctgccatcaa cctccagggt cgcctgaccg gtactgaaat ccgccttgaa
     5401 tttcaccgta accgtcttct gagcattatt cactgaggcc gtgatgctgt gttcccccgc
     5461 aaccgttgac ataagctcaa tgtccacttt ccctgccgca ttggttgtta ctgtattccc
     5521 cgtcttgctg gtcacaccct gcggtaaagt cagcgtaact tcttgcccct gcaacgggtt
     5581 accatagctg tccacgacgg tcagggttat ctggttagca gactgtccat tagccagttg
     5641 gttattaacc ttcactgtca tatcacgaat ctcagcctta gatgcgtcac ctgcaacgtt
     5701 cagcaccagt ggctgagcaa cggcattttg gccgttcact cgcggcatca cagacaactg
     5761 acccgcggca gatcccagcg ttaaggtcgc cacgtagacc ccattacctt tctctgtcca
     5821 gtttcctgct gaaggacgct cactccccgt gctggcggca ccgctaaaca ctggtgcatc
     5881 tggcttcagc ccggtgaccg ggttcccgta cgcatccttc acggtgaagg tgagttccgt
     5941 cgtcattttg acggtcggag ccttattgtc cgcaacaagc gtagagttcg ctgatgacat
     6001 ctctccggcg atgaccgtca actgcgcggc ttcggtggct gctggctggc cgttgaagag
     6061 cggcatgaca cgaagctcgc ccgtctttcc gcctgtagtt aacgtagcaa cataggaacc
     6121 gtcacctttt tcggtccaac tggaaacggt cgccccttca gaggcggtcc ccgtcaaact
     6181 tgccgacaac gaaagaccac tgatagtgtt gccatgtgca tcttttgcaa taagcgtcac
     6241 ggttgtgctt tcgccggctt ttacgtgatc ttctgcgaca gagactttcg actggtttga
     6301 agataacgca tcagccacca cggttacttt tgccgcattt gctgccgcat cctgtccatt
     6361 tagcttcggc ataacttcta attcacccgc cgtagagccg agagtaatct gcgcagtcca
     6421 cgtcccatca tcattatttg tccagccaga tgccgtagaa cctacagcag cggcacccgc
     6481 taatgacggc gcttccggcg tgaggctggt cacagggttg tcataggcat cttttgccgt
     6541 ccagattgcc gtaactgtac cgccaaccac cggtttatca ggattcaggg tgatggacga
     6601 atgtgctgca tcaagcggac cggcaacaaa cttcagcgtt tgttgtaatg tgcccagctg
     6661 atactgctcg ctgaaagcac gcacaacaac gtttccggca cgggtcgaag aaaccgtggc
     6721 gctgtaaacg cctggtttta tttccgaaat ggcaccaacg gttacaccat tagtgtcttg
     6781 cggaacaaat cgcaagcggc tggcttctcc cgtcaccgga ttaccctcgg agtccaccgc
     6841 agtcaacgtc aacgtatagg cttgctgacc atcagcaacc acatcacctg acggctcgtt
     6901 agcgctcagg gtggagtttg ccacatccat catcgtggcc cgcagttctg cagtgacggt
     6961 tttgctcatg ccatcaacgc taacagtaat cgttgcctca cctgactgcg ttccggtagt
     7021 aaagacagac tgatacaccc ctgcttcagt ttcggtgaac tcacccagtg ttggctttgc
     7081 ctgtgattta gtggccttca gggaacgagt cacaatattt ccagccggtt tgaaggctag
     7141 ttcagtcttg atctgatctt tcatgcccgt gactggctgc ccctcggcgt cgcgcagaga
     7201 cagcaccagc ggtctttgct cattaccgtt agcaagcatt tgaatacggc tctgaccgtc
     7261 aagcgttaac gccgtgcgat cggcgctcat acctgctccg gtaatgacca cctctgtctg
     7321 cacgcgtttt gaggcattgc ctttgttatc gtaggcaacc gcagaaatcg cataataatt
     7381 gtctttgcct ggacgataag ccgggagcgt tacttgccac t
//
LOCUS       DAMZET010000189         7333 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.189, whole genome shotgun sequence.
ACCESSION   DAMZET010000189 DAMZET010000000
VERSION     DAMZET010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.189"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1116
                     /gene="wcaC"
                     /locus_tag="POB34_004103"
     CDS             <1..1116
                     /gene="wcaC"
                     /locus_tag="POB34_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HDI5920958.1"
                     /translation="FVYGYGKGGKESVSHQNYPQVIKHTPRMTAMANIVLFRLFNRDL
                     FGNFNELYRTITRTPGPVVLHFHVLHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDH
                     WSVTGRCAFTDGCEGWKTGCQKCPTLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQF
                     ISPSQHVADAFNSLYGPGRCRIINNGIDMATEAILADLPPVRETQGKPKIAVVAHDLR
                     YDGKTNQQLVREMMALGDKIELHTFGKFSPFTAGNVVNHGFETDKRKLMSALNQMDAL
                     VFSSRVDNYPLILCEALSIGVPVIATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPE
                     IAQAIFGTTLAEFSQRSRAAYSGQQMLEEYVNFYQNL"
     gene            1091..2308
                     /gene="wcaD"
                     /locus_tag="POB34_004104"
     CDS             1091..2308
                     /gene="wcaD"
                     /locus_tag="POB34_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HDI5920959.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            2319..3065
                     /gene="wcaE"
                     /locus_tag="POB34_004105"
     CDS             2319..3065
                     /gene="wcaE"
                     /locus_tag="POB34_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HDI5920960.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNSIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            3081..3629
                     /gene="wcaF"
                     /locus_tag="POB34_004106"
     CDS             3081..3629
                     /gene="wcaF"
                     /locus_tag="POB34_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HDI5920961.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            3655..4776
                     /gene="gmd"
                     /locus_tag="POB34_004107"
     CDS             3655..4776
                     /gene="gmd"
                     /locus_tag="POB34_004107"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HDI5920962.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            4779..5744
                     /gene="fcl"
                     /locus_tag="POB34_004108"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             4779..5744
                     /gene="fcl"
                     /locus_tag="POB34_004108"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HDI5920963.1"
                     /translation="MSKQRVFIAGHRGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASESIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVDYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENRDRFRG"
     gene            5747..6226
                     /gene="gmm"
                     /locus_tag="POB34_004109"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             5747..6226
                     /gene="gmm"
                     /locus_tag="POB34_004109"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HDI5920964.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIAENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEDLILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            6223..>7333
                     /gene="wcaI"
                     /locus_tag="POB34_004110"
     CDS             6223..>7333
                     /gene="wcaI"
                     /locus_tag="POB34_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HDI5920965.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAG"
BASE COUNT         1768 a         1778 c         1989 g         1798 t
ORIGIN      
        1 tttgtctacg gttacggcaa aggcggcaaa gagagcgtca gccatcaaaa ctatccgcag
       61 gtcatcaaac atacgccgcg gatgactgca atggcgaata ttgtcctgtt tcgtctgttt
      121 aatcgcgatc tgtttggcaa tttcaatgag ttatatcgca ccattactcg cacaccgggt
      181 ccggtggtcc tgcattttca tgtgctgcac agctactggc taaatcttaa gagcgtggtg
      241 cgcttttgcg aaaaagtgaa aaaccataaa ccggacgtca ctctggtctg gacgctgcac
      301 gaccactgga gtgttaccgg acgctgcgcc tttaccgacg gttgcgaagg ctggaaaaca
      361 ggctgccaga aatgcccgac cttaaataat tatccgccgg tgaagattga tcgggcgcac
      421 cagctggtgg cgggcaaacg ccagttattc cgtgagatgc tggcgctggg ctgtcagttt
      481 atttccccca gccagcatgt ggctgacgct ttcaatagcc tgtacggtcc agggcgttgc
      541 cggattatca ataacggtat tgatatggca accgaagcga ttctggcgga tctgcctccg
      601 gtgcgcgaaa cccagggtaa gccgaaaatc gcggtggtgg cgcatgacct gcgttacgac
      661 ggcaagacta accagcaact ggtgcgtgag atgatggcgc tgggcgacaa aatcgaactg
      721 catacctttg gtaagttctc gccgttcacc gctggtaacg tggttaatca cggctttgaa
      781 accgacaaac gtaagctgat gagcgcgctc aatcagatgg atgcgctggt attcagttct
      841 cgcgtcgata actacccgct gattttgtgt gaggcgctat cgattggcgt gccggtgatc
      901 gccacccata gcgatgcggc gcgggaagtg ctgcaaaaat ccggtggtaa aaccgtcagt
      961 gaagaagagg tgctgcaact ggtgcagtta agcaaaccgg aaatcgcgca ggcgatattt
     1021 ggtaccacgc tggctgagtt cagccaacgc agccgcgccg cctacagtgg acaacagatg
     1081 ctggaggagt atgtcaactt ctatcagaat ctgtagctac ctgctgctgc cgctgatcta
     1141 cctgctggtt aacgtcaaaa tcgcccagct tggtgaaagt ttccccatca ctatcgtcac
     1201 ttttttacct gtcttgttac tgctgttttt agaacgcatc agcgttaaaa aattgatgat
     1261 tgccttaggg attggtgcgg gactcacggc gttcaactat ctgtttggtc agtcgctgga
     1321 tgccagtaaa tacgtcactt caaccatgct gtttgtctat attgtgatca ttattggcat
     1381 ggtgtggagt attcgcttta aaacgatttc gccacacaac catcgcaaga tattacgttt
     1441 cttttatctg gtggtcgggt tggtggtggc gctggcggcg gtggagatgg cgcaaattat
     1501 cctcaccggc ggcagcagta ttatggagtc gatttcgaaa tatctgattt acagcaacag
     1561 ctatgtactg aatttcatta aattcggcgg caagcgcacg acagcacttt atttcgaacc
     1621 ggcatttttc gctctggcat taatctcaat ttggctcagc atcaaacagt ttggtatcaa
     1681 aacgcctaaa accgatgcta tgattctcgc agggataata ttatccggat cgttttcagg
     1741 ggttatgacc tttatcctgt tttatttgct ggagtgggca tttcaatatc tgaataaaga
     1801 ggcgattaag aaaaagttac cgttagcatt gatttctctg gctgtattcc tggttggtgt
     1861 ggtaatcgcg tttccttata tttccacccg tctgggcgat ttaggtacgg aaggatcgtc
     1921 atcgtattat cgtattgtcg gtccgctggt gatggtcggt tattctttga cccatattga
     1981 cggtgtagtc agatttggct cactttatga atatgtcgca tcattcggaa tatttaacgg
     2041 tgcggatgtc ggaaaaacca tagacaatgg tttgtatctg ctgattattt atttttcctg
     2101 gttcgcggtg tttttatcgc tgtggtacat ggggaaagtg ataaaaatga tgatcaacgc
     2161 ttttggtgat aaccgcaatt ttcgcgtgca attatatctt tttactccgg tatcgctgtt
     2221 ttttaccggt tcgatattta gcccggaata tgcattttta atcgtctgtc cgtttatttt
     2281 gcgaaaagcg ttaaatatta cgaggtaaga ataagaacat gttgcttagc ataatcactg
     2341 tcgcgtttcg taacctcgaa gggatagtca aaacacatgc ctcgctggcg catctggcgc
     2401 aggcggacga tatcagcttc gaatggattg ttgtcgatgg cggttccaac gacggtaccc
     2461 gtgagtatct ggaaaatctc aatagtatct ataacctacg ctttgtcagc gagccagata
     2521 acggtatcta cgacgccatg aacaagggca ttgcgatggc acaaggcaag ttcgcgttgt
     2581 ttctcaattc gggcgatatt tttcatcagg atgccgcaaa ttttgtccgt aagttaaaaa
     2641 tgcaaaaaga taacatgatg atcaccggcg atgcgctgct ggattttggc gacgggcata
     2701 aaattaagcg tagtgctaaa ccgggttggt atatttatca cagcctgccc gccagccatc
     2761 aggcgatatt tttcccggta tccggtttga aaaaatggcg ttatgacctg gaatataaag
     2821 tttcttctga ttacgcgctg gcagctaaaa tgtataaagc aggttatgca tttaaaaaac
     2881 tcaatggcct ggtgtctgaa ttttccatgg gtggggtatc aaccaccaat aatatggaat
     2941 tgtgtgctga cgccaaaaaa gtccaacggc agatattaca tgtgcctggc ttctgggcag
     3001 aattatcctg gcatttacgc caacgtacta cctcaaagac gaaagcctta tataacaaaa
     3061 gctgaatata agggaaaacg atgcaggatt taagcggttt ctcggtgccg aaagggttcc
     3121 ggggcggcaa cgctattaaa gtgcaattat ggtgggcagt acaggcaaca atatttgcct
     3181 ggtcgccaca agtattgtat cgctggcggg cttttttatt acgtttattc ggagcaaaaa
     3241 taggaaaaaa cgtagttatt cgtccgtcag taaaaattac ctatccgtgg aaattaacct
     3301 taggtgatta cgcgtgggtc ggcgatgacg tcaatttata taccctcggt gaaataacca
     3361 ttggcgcaca ttcggtgata tcgcaaaaaa gttatttatg caccggtagc cacgaccatg
     3421 caagtcaaca tttcaccatt aacgccacgc ctattgtgat tggcgagaaa tgctggctgg
     3481 caaccgatgt ctttgttgcc ccaggcgtca caatcggcga cggcaccgtc gtgggtgcac
     3541 gaagcagtgt ttttaaatcg cttccggcaa atgtggtttg ccgggggaat cccgcagtgg
     3601 tgatacgcga acgcgttgaa actgaataaa ttcaaaaata cagaggaata agacatgtca
     3661 aaagtcgctc tcatcaccgg tgtaaccgga caagacggtt cttacctggc agagtttctg
     3721 ctggaaaaag gttacgaggt gcatggtatt aagcgtcgcg catcgtcatt caacaccgag
     3781 cgcgtggatc acatttatca ggatccgcac acctgcaacc cgaaattcca tctgcattat
     3841 ggcgacctga gtgatacctc taacctgacg cgcattttgc gtgaagtaca gccggatgaa
     3901 gtgtacaacc tgggcgcaat gagccacgtt gcggtctctt ttgagtcacc agaatatacc
     3961 gctgacgtcg acgcgatggg tacgctgcgc ctgctggagg cgatccgctt cctcggtctg
     4021 gaaaagaaaa ctcgtttcta tcaggcttcc acctctgaac tgtacggtct ggtgcaggaa
     4081 attccgcaga aagaaaccac gccgttctac ccgcgatctc cgtatgcggt cgccaaactg
     4141 tacgcctact ggatcaccgt taactaccgc gaatcctacg gcatgtacgc ctgtaacggt
     4201 attctcttca accatgaatc cccgcgccgc ggcgaaacct tcgttacccg caaaattacc
     4261 cgcgcaatcg ccaacattgc ccaggggctg gagtcgtgcc tgtacctcgg caatatggac
     4321 tccctgcgtg actggggcca tgccaaagac tacgtaaaaa tgcagtggat gatgctgcaa
     4381 caggaacaac cggaagattt tgttatcgcg accggcgttc agtactccgt acgtcagttc
     4441 gtggaaatgg cggcagcaca gttgggcatc aaactgcgct ttgaaggcac gggtgttgaa
     4501 gagaagggca ttgtggtttc cgtcaccggg catgacgcgc cgggcgttaa accgggtgat
     4561 gtgattatcg ctgttgaccc gcgttacttc cgtccggctg aagttgaaac gctgctcggc
     4621 gacccgacca aagcgcacga aaaactgggc tggaaaccgg aaatcaccct cagagagatg
     4681 gtgtctgaaa tggtggctaa tgacctcgaa gcggcgaaaa aacactctct gctgaaatct
     4741 cacggctacg acgtggcgat cgcgctggag tcataagcat gagtaaacaa cgagttttta
     4801 ttgctggtca tcgcgggatg gtcggttccg ccatcaagcg acagctcgaa cagcgcggtg
     4861 atgtggagct ggtattacgc acccgcgatg aactgaacct gctggacagc cgcgcggtgc
     4921 atgatttctt tgccagcgaa agcattgacc aggtctatct ggcggcggcg aaagtgggcg
     4981 gcattgttgc caacaacacg tatccggcgg atttcatcta ccagaacatg atgattgaga
     5041 gcaacatcat tcacgccgcg catcagaacg acgtgaacaa actgctgttt ctcggatcgt
     5101 cctgtatcta cccgaaactg gcaaaacagc cgatggcaga aagcgagtta ttgcagggca
     5161 cgctggagcc gaccaacgag ccttatgcca ttgccaaaat tgccgggatc aaactgtgcg
     5221 aatcttacaa ccgccagtac ggacgcgatt accgctcagt catgccgacc aacctgtatg
     5281 gcccgcatga caacttccac ccgagtaatt cgcatgtgat cccagcattg ttgcgtcgct
     5341 tccacgaggc gacggcacag aatgcgccgg acgttgtggt atggggcagc ggtacaccga
     5401 tgcgtgaatt cctgcacgtc gatgatatgg cggcggccag cattcacgtg atggagctgg
     5461 cgcacgaagt ctggctggag aacacccagc cgatgctgtc gcacattaac gtcggtaccg
     5521 gtgttgactg cactatccgc gagctggcgc aaaccatcgc caaagtggtg gattacaaag
     5581 gtcgggtggt ttttgatgcc agcaagccgg atggtacgcc gcgcaaactg ctggatgtga
     5641 cgcgcctgca tcagcttggc tggtatcacg aaatctcact ggaagcgggg cttgccagca
     5701 cttaccagtg gttccttgag aatcgagacc gctttcgggg gtaatgatgt ttttacgtca
     5761 ggaagacttt gccacggtag tgcgctccac accgcttgtc tctctcgact ttattgccga
     5821 gaacagtcgc ggcgagtttc tgcttggcaa aagaaccaac cgcccggcgc agggttactg
     5881 gtttgtgccg ggagggcgcg tgcagaaaga cgaaacgctg gaagccgcat ttgagcggct
     5941 gacgatggcg gaactggggc tgcgtctgcc gataacagca ggccagtttt acggtgtctg
     6001 gcagcacttt tatgacgata acttctctgg cacggatttc accactcact atgtggtgct
     6061 cggttttcgc ttcagagtcg ctgaagaaga tctgatactg ccggatgagc agcatgacga
     6121 ttaccgctgg ctgacgccgg acgcgctgct cgccagtaac gatgttcacg ctaacagccg
     6181 cgcctatttt ctcgctgaga agcgtgccgg agtacccgga ttatgaaaat actggtctac
     6241 ggcattaact actcgccgga gttaaccggt atcggcaaat acaccggcga gatggtggaa
     6301 tggctggcgg cgcaaggtca tgaggtgcgg gttattaccg caccgcctta ctacccgcag
     6361 tggcaggtgg gcgagaacta ttccgcctgg cgctacaaac gagaagaggg ggccgccacg
     6421 gtgtggcgct gcccgctgta cgtgccaaaa cagccgagca ccctgaaacg cttgttgcat
     6481 ctcggcagtt ttgccgtcag cagtttcttt ccactgatgg cgcaacgtcg ctggaagccg
     6541 gatcgcatta tcggcgtagt gccaacgctg ttttgcacgc cgggaatgcg cctgctggcg
     6601 aagctctctg gtgcgcgtac cgtgctgcat attcaggatt acgaagtgga cgccatgctg
     6661 gggctgggcc ttgccggaaa aggcaaaggc ggcaaagtgg cacagctggc aacggcgttc
     6721 gaacgtagcg gactgcataa cgtcgataac gtctccacga tttcgcgttc aatgatgaat
     6781 aaagccatcg aaaaaggcgt ggcggcggaa aacgtcatct tcttccccaa ctggtcggaa
     6841 attgcccgtt ttcagcatgt tgcagatgcc gatgttgacg cccttcgtaa ccagcttgac
     6901 ctgccggata acaaaaaaat cattctttac tccggcaata ttggtgaaaa gcaggggctg
     6961 gaaaacgtta ttgaagctgc cgatcgtctg cgcgatgaac cgctgatttt tgccattgtc
     7021 gggcagggcg gcggcaaagc gcggctggaa aaaatggcgc agcagcgtgg actgcgcaac
     7081 atgcaatttt tcccgctgca atcgtatgac gctttacccg cactgctgaa gatgggcgat
     7141 tgccatctgg tggtgcaaaa acgcggcgcg gcagatgccg tattgccgtc gaaactgacc
     7201 aatattctgg cggtaggcgg taacgcggtg attaccgcag aggcccacac agaactgggg
     7261 cagctttgcg aaacctttcc gggcattgcg gtttgcgttg aaccggaatc ggttgaggcg
     7321 ctggtggcgg gga
//
LOCUS       DAMZET010000190         7316 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.190, whole genome shotgun sequence.
ACCESSION   DAMZET010000190 DAMZET010000000
VERSION     DAMZET010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.190"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(67..555)
                     /gene="wcaB"
                     /locus_tag="POB34_004111"
     CDS             complement(67..555)
                     /gene="wcaB"
                     /locus_tag="POB34_004111"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HDI5920966.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNIWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(558..1397)
                     /gene="wcaA"
                     /locus_tag="POB34_004112"
     CDS             complement(558..1397)
                     /gene="wcaA"
                     /locus_tag="POB34_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HDI5920967.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(1575..3737)
                     /gene="wzc"
                     /locus_tag="POB34_004113"
     CDS             complement(1575..3737)
                     /gene="wzc"
                     /locus_tag="POB34_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HDI5920968.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(3740..4183)
                     /gene="wzb"
                     /locus_tag="POB34_004114"
     CDS             complement(3740..4183)
                     /gene="wzb"
                     /locus_tag="POB34_004114"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HDI5920969.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(4189..5328)
                     /gene="wza"
                     /locus_tag="POB34_004115"
     CDS             complement(4189..5328)
                     /gene="wza"
                     /locus_tag="POB34_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HDI5920970.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            5637..5786
                     /locus_tag="POB34_004116"
     CDS             5637..5786
                     /locus_tag="POB34_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920971.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            5987..>7316
                     /locus_tag="POB34_004117"
     CDS             5987..>7316
                     /locus_tag="POB34_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDI5920972.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNAD"
BASE COUNT         1635 a         1897 c         1879 g         1905 t
ORIGIN      
        1 ggtggagatc taacgccaca cctgctgccc cgccttccgc cagtcgcaca ttaaattgca
       61 aaatattcat ttaattacct tcactcgcgc tttttctccc accaccagcg cgttgtccgg
      121 gacagagtcg agcaccacgc tgcccgcacc cacggtgacg ttgttaccaa gcgtgatatc
      181 accaagaata ataacgttgg caccgagttc gacgccgttg ccaatatgtg gacatgccat
      241 gttatcggca ccacgattgc cgatagtgac gccgtggcga atggtgaaat catcccccgc
      301 caccacgttt ttattgatca ctacggcgta accgtgatgg atagtaaagc ggcggccaat
      361 ggtcgcggca gcctggattt cataaccgaa aaagcattcg gtgataatgc gatacagcac
      421 cagcagcggg gccgcccaaa tattgttgag gacgttcttt ttgcgccaca ccgaacaaaa
      481 atgagcgacg cgataggcta gaaccatgca gcacggacgt aaactccagc tgttggcgcg
      541 cagatcttcc agcatcttta gcgcccccga ataccatcag ccagacgttt gccgttacgc
      601 accgaaagca gcgtcagcaa agtgcgccag gtcatacgtt tattgcggat ctggtagagg
      661 gtaaacagct gatatttttt gcttgcacgg tcaaatttat ctttgtgctt gcggtaaaag
      721 tggaagtacc cggagaattt tttcggtgac gaggtgatct gcatttcacc gtgattgata
      781 tgcaggatct gcgtcgcctc ttccactttc cacggctcgc catactccac caccatccgc
      841 aggaagatgt cgtaatcctg tgctgctttc agttcggtat cgaacaagca ctctttgaaa
      901 cgccacgccc aggtaaaaac ctgattgcca atgatattgc gtttatagaa caggcggcgt
      961 gagtacggcg atttgggata cagtggtaag cttgccggtt gggaataaac ttctccctgg
     1021 catacgtagt cgttagcgta caaaaaggcg tgagtgacca gttgctgttt atgggcgagg
     1081 aagacgctca ggcggttggg cgtccattca tcatcgtcat cgatcccggt aatgtattcc
     1141 ccttgcgcca gcataatcgc ctggttacgt accgcgcacg ccccgctgtt aatgtcgttg
     1201 tgaatgtagg taatacgcgg atcgttgagg gcggtgacgt actgttgcag ttgttcccag
     1261 gaggtggagc aatcatccac gatgatcatc tcccagttgc tgtagtcctg gcgcaggacc
     1321 gattttatcg cccgaatcgc cagttgttgg cggttccagg tcggcatata gattgagatc
     1381 agcggattgt ctttcatagg ttgttctccc ggagtgtcga gatgttctgc cggtttataa
     1441 gcttgtttac gggttctgtt gccggatgcg gcgtgagcgc cttatccggc ctacggagct
     1501 ggtttgcttg ccggatgcgg cgtgaaggta ggtcggataa gatgcgtcag catcgcatcc
     1561 gacagcggat gtcgttattt cgcatccgac ttatattcgt attcgtaata gccataatcc
     1621 tgatacgcgc tggcgcggcg gaagatggag ttcagaatca cccctttcac cggaatacca
     1681 ttttgctcaa agcggctcag actggtttcc acttctttca atgtgttgac cgcataacgc
     1741 gccaccatta acgtggttcc aacatgacga ccaacaattg ccgcatcggt cactgccaga
     1801 atcggcggcg tatcaatcaa caccaggtca tagtttttac tcgcccagtt caccagttcg
     1861 gcaaagcgtt cgctcatcaa cagctcagaa ggatttggcg gtacctgacc gcgcgggatc
     1921 aggtcaaatt tggcaataga ggtcggttta gctgcggtag taatatcgcc ctggccaatc
     1981 agaatttccg acaggccatt gacgttattg gtacccaaca gctcgtgggt gtagcctttg
     2041 cgcatatcgc agtcgatcaa caacacccgt ttattggtct gactgatcac cgccgccagg
     2101 ttggcgcaga caaaggtttt accgattgac gggctcaccc ctgtcatcat caacacattg
     2161 ttctgcgcct gcatcatcgc gaagtgcaga ctggtacgca gactgcggat ggcttcaatc
     2221 gccagatcgg ttggattccc caccgccagt agctggctct gtttatagcg tttaatccct
     2281 ttgatggttt tgacgctatc gcgcgctttc tgccattccg acaacgggat gctggcatag
     2341 acgctgatac catgttcttc cagcacctgc gggctttcga tgccgcgatt aaacaacgag
     2401 cgcagcagca cacccacgat agagagcatc aggccgagga taattgcgcc gaggataatc
     2461 agtcctttct tcggtttcag cacgccaggc tgggtgattg ccgggtcaac aatgcgcaca
     2521 tcgccgacgg tgctggcttc ggtgattttc agctcctgct ctttattcag cagttgcata
     2581 tagacctgct gaccagactc gacatcgcgg gtcaggcgca caatctcctg ctgggttttc
     2641 ggcatcgccg ttacgcgacc gttaagtttg gctttctctt cttccagcgc ctgacgtttc
     2701 tccagcagcg tgcggtacgc cgggtgaact ttggtgtaca gcttggagat ttccgcctct
     2761 ttaaaggtca gttcgttcaa ctgcgcgtcg atgttcacca tcgaatcgag caccgctttc
     2821 gcttccagcg gcagatcaac agaatcttta tcctgacgga aggcattcag tttgttttcg
     2881 gcaacatcga ggcggctacg tacttccggt aactgttgcg cgaggaaagc gaggcttttc
     2941 gacgcttccg ccgatttgcg ctcaatattt tgttcctgat agttacgggc gatgctgtta
     3001 agaatgtcgc ggatctgttc gcgatcttca ccggtataag tcaggctcag tacgcctgcg
     3061 tctttgccgt tctccgttac cgtcaggctg ttttgcagtt ggttgatcat ccccaacgtg
     3121 gagtatttgg tgacggtaaa ctcactgccc gggcgggcgt gaatggcttc aaccatcagc
     3181 gtgacgcctt cttttttcag catctggccc gcttgcccac gggcgctaaa gccgccatca
     3241 ctgctcagag tgtagttttt gtcatccagc acattaagcg taaacacctg gtccgccatc
     3301 tctttcgggc ggttaaaggt ggtcactttc accgtctcgt tctgacgtcc catcaggcga
     3361 tcccaacccg caccgaaaat cgggaacgtg tttttgctca ccgcaatatc gaggtctaga
     3421 tcgtccaccg ttttaccaag caccagacgc gaacgaatca actggatctc ggcgtccgat
     3481 gcaggcggtt tgttggctaa tgccgagcca atgtcctgca ctaacgaatt gccgctgttt
     3541 tgttcgattt gtaccagtgc gtcggcacta taaatcggcg tggcgaagaa agtgtaaacc
     3601 acagcacaga gggcaaatac agcggtgatg ccaatcaccc accagcgcgc ttcaatgacg
     3661 gtgccgacca ggcgaccaat atcgatttca tcactgcccg ttaccggagc ggcatgttgt
     3721 tttacttttt ctgtcattct tatacctgct ctgcgttcaa tgcctgcgcc cactggcggg
     3781 cagaccgttc aagtaatgtg tacaccgctg caaacgtttc ccggcttttg cgatacggat
     3841 cggggatttc acattcgtta tcccagtgac caaacagcat cactttgccg cgcatctcag
     3901 gtgccatctc gcataagcgt tcgatatggc gcttttccat ggtcaaaatc aggtcatagt
     3961 tgcgacacag gcggcggctg atttgccgtg cacagtgacc ttccagagac agttgatgtt
     4021 ctgcggcgac gctgatagcg gtaggatcag cgcccttacc gaccagcgcg ccgagtccag
     4081 cggactccac tttcagctcc gggtgataac gttgcagtaa gcgttccgcc gtcggggaac
     4141 ggcaaatatt gccgacacag acaactaata tgttgttaaa catgacgatt accagttatg
     4201 aatgtcgctg gctgtatccg tcatgtaacg gacaccgcta atagttggca gcaactgatt
     4261 gatcagacgg ttccagcggg aaaccggggc ggtggtgaca tacaccacgt cataaggttg
     4321 caggcggaat tctgtcgcca tcaccagcga cgtcgcatcg gacatatcca gctggtagat
     4381 attggcaatc ttgccgttac gcccgccctc gcctttcaac ggacgaatga caaagatgcc
     4441 gctggcgttg gaggtggtca tgtcgatacc ttcggcattg cccagagctt cagtcagggt
     4501 catgccgcta aagtccattt tcagggtgct ctgtttcttc acttcaccca tcacaaacac
     4561 tttcagatca tcattacgcg gcacgtagag aatatcgccg gggtaaagca gtcggttctg
     4621 attgaggtcg ccgttttgca tcagcgcctg caaagaaatg cgttcttcac gaccattgtg
     4681 tgttagcacc acgttgcgcc agtcagcggt gtcggtcagg ccacctgcgg cgttgatggc
     4741 atcgagaatg gtcagtggta cgttggtgat cgcctgttga ccggatttat tcacctgacc
     4801 tgagatatag gccttttgtg agcggaaggc ggcgatatta acgtccacct gcgggtcagc
     4861 gatgtacgtc gctaagcgcc cggtaatatc actgcggatt tcagcgagcg ttttcccgac
     4921 tacgtgaacc ttgccgatat acgggtaaaa catagtgccg tcaggctgta cccagttgcc
     4981 ggtgtcgctg gagctgcggt actgaccggc tggcgtggtg agttccgggt gatcccagac
     5041 ggtgacattc agaacgtccc ccggcccgac gcgatactgg taattcgcga tctcgctttc
     5101 cagcgtcata ttggggcgcg ctacattcgg gcgtgggcgt aattggtcaa tcaggcgcgg
     5161 ggtcagcgga taaacattca ccattttgtc gagatcgaaa tcagcgtcct gctgtttgat
     5221 gacgtctttg cccatcgtcg acatattgct gcccggaagt actgtgcaac cgcttatcaa
     5281 ggttactgac accaataatg gcatcaattt cattttggat ttcatcattg tttatttatc
     5341 actttggcag agtaattatc ctgtgcacta ttaatagcaa tgtcgccatg cacatttacc
     5401 ttgcagttaa ttgaataaaa atttaactgg catcagtcct aaaaaaattg atttcatccg
     5461 caggctattg acagaataat tcagactggt ctttcaggca tccagacacg ctaccgcccc
     5521 tggcttttta gctaccaata cactgattta gcttaatttt tcaaaccctc tccgcataca
     5581 atcgttgatg agaaagggtt attgcggaaa ttaacttccg aatataaggt gacattatgg
     5641 taattgaata ttggctctcc aataatgcag gaggaagtgt tacagctaac ggaatagcag
     5701 gcaaggtaac aattcggcaa ttggctattt ttaagaatta tattaagtgt cattcaatat
     5761 ggtttttagg agtttcttta ggttgacaat atttaatatt gcgtctccac atgcgatatt
     5821 tcttagataa tgctttatta ttaccacttt taattcaggg ataatgtgag gttattaccc
     5881 tcaaataata tgagataaat agtgctgcaa cattgcattt ttgccccgat ttatccatga
     5941 tcgaattgtg acatttgtca tacaacgaat aggttttgta cttactatgg aatggattgc
     6001 cgatccgtct atctgggccg ggttaatcac gctgattgtg atcgaactgg tcctcggcat
     6061 tgataacctg gtctttattg ccatcctcgc cgaaaaacta ccgccgaagc agcgcgaccg
     6121 cgcacgggtt accgggctgc tgctggcgat gttaatgcgc ctgttactgc tggcgtcaat
     6181 ctcctggctg gtcaccctga ctcaaccgct gttcagcttc cgctcgttta cctttagcgc
     6241 ccgcgactta atcatgctgt ttggtggttt cttcctgttg ttcaaagcca cgatggagct
     6301 gaacgaacgg ctggaaggga aagacagcaa taatcccaca caacgcaaag gtgcgaagtt
     6361 ctggggggtg gtgacacaaa ttgtggtact ggacgccatc ttctcactcg actcggtgat
     6421 taccgccgtc gggatggtcg accatttact ggtcatgatg gccgccgtgg ttatcgctat
     6481 cagcctgatg ttgatggcaa gcaagccgtt aacccagttc gttaatagcc acccgaccat
     6541 cgttattctc tgcttaagct tcctgctgat gattggcttt agcctggtgg cagaaggttt
     6601 cggcttcgtc attccgaaag gctacctgta cgctgccatt ggtttctcgg tgatgatcga
     6661 ggcgctcaat cagttggcta tctttaaccg gcgacgtttt ctttccgcta accagacgct
     6721 gcgccagcgg actaccgaag cggtaatgcg cctgcttagc gggcaaaaag aagatgcgga
     6781 actggacgcc gaaaccgcgt ccatgctggt ggatcatggt aaccagcaga tctttaatcc
     6841 gcaggaacgg cggatgattg agcgggtact taatcttaac cagcgtaccg tcagcagcat
     6901 tatgacgtcg cgccacgata ttgagcatat cgatctcaac gcgccggaag acgagatccg
     6961 ccagttactg gagcgaaatc agcatacgcg gctggtggtt accgacggtg atgacgcaga
     7021 agatttgctc ggtgttgttc acgttatcga cctgctacaa cagtctctgc gcggcgaacc
     7081 gctcaacctg cgggtgttga ttcgccagcc gctggtgttc ccggaaacct tgccgttgtt
     7141 acctgccctg gagcagttcc gtaatgcccg cacgcacttt gcttttgtgg tggatgagtt
     7201 tggctcggtg gaagggattg tgacattaag tgacgtcact gaaaccattg ccggtaactt
     7261 accgaacgaa gtggaagaga tcgacgcccg ccatgatatt cagaagaatg cggacg
//
LOCUS       DAMZET010000191         7217 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.191, whole genome shotgun sequence.
ACCESSION   DAMZET010000191 DAMZET010000000
VERSION     DAMZET010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.191"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..200
                     /locus_tag="POB34_004118"
     CDS             <1..200
                     /locus_tag="POB34_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2919 family protein"
                     /protein_id="HDI5920973.1"
                     /translation="PRTWRVLYFLLLLAQVVLLCWQPWLWLNGESVSGIGLALVVADI
                     VALIWLLTNRRLRACFNEVKE"
     gene            261..836
                     /locus_tag="POB34_004119"
     CDS             261..836
                     /locus_tag="POB34_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HDI5920974.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            932..1831
                     /gene="yfeX"
                     /locus_tag="POB34_004120"
     CDS             932..1831
                     /gene="yfeX"
                     /locus_tag="POB34_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HDI5920975.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(1889..3193)
                     /gene="pbp4b"
                     /locus_tag="POB34_004121"
     CDS             complement(1889..3193)
                     /gene="pbp4b"
                     /locus_tag="POB34_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HDI5920976.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(3198..4622)
                     /gene="murP"
                     /locus_tag="POB34_004122"
     CDS             complement(3198..4622)
                     /gene="murP"
                     /locus_tag="POB34_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HDI5920977.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(4626..5522)
                     /gene="murQ"
                     /locus_tag="POB34_004123"
     CDS             complement(4626..5522)
                     /gene="murQ"
                     /locus_tag="POB34_004123"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDI5920978.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            5686..6543
                     /gene="murR"
                     /locus_tag="POB34_004124"
     CDS             5686..6543
                     /gene="murR"
                     /locus_tag="POB34_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HDI5920979.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            6672..>7217
                     /gene="ucpA"
                     /locus_tag="POB34_004125"
     CDS             6672..>7217
                     /gene="ucpA"
                     /locus_tag="POB34_004125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HDI5920980.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNA"
BASE COUNT         1749 a         1848 c         1966 g         1654 t
ORIGIN      
        1 ttcctcgcac ctggcgtgta ctctattttc tgttgctgtt ggcgcaggtg gttttacttt
       61 gctggcaacc gtggctgtgg ctgaacggtg aatccgttag cggtatcggt ctggcgctgg
      121 tagtggcgga tattgtggcg ttaatctggc tgctgaccaa tcgacgttta cgcgcttgtt
      181 ttaatgaagt aaaagaataa cggcactttt tggtgaattt gcactccaag caacgttatt
      241 gaataaccaa aggaagtgac atgaaatcgc tgcgtttaat gttatgcgct atgccattga
      301 tgctgaccgg ctgttccacg atgtcgtcag ttaactggtc tgccgctaac ccgtggaact
      361 ggtttgggtc gtccaccaaa gtgagcgagc agggcgttgg tgaattaacg gcgtccacac
      421 cactgcaaga acaagccatt gccgatgcgc ttgatggcga ttatcgcctg cgcagcggaa
      481 tgaaaaccgc gaacggcaac gtggtgcgct tttttgaagt gatgaagggc gacaacgtgg
      541 cgatggtgat taacggcgat caaggcacga tcagtcgcat tgacgtgctg gatagcgata
      601 ttcctgctga caccggtgtt aaaatcggta caccgtttag cgacctttac agcaaagcat
      661 ttggcaattg ccaaaaagct gatggtgatg ataatcgtgc cgtcgaatgt aaagccgaag
      721 gcagtcaaca tattagctac cagttcagcg gggaatggag tggtcctgaa gggttaatgc
      781 cttcggacga taccctgaaa aactggaaag tcagtaaaat tatctggcgg cgttaatttg
      841 cgtctgaaca aaccgccgcc gcgaaaaaac acgtaaaata gcgctcaaca atgccacgga
      901 ttgcgtggca ttattatttt caggaggaac aatgtctcag gttcagagtg gcattttgcc
      961 agaacattgc cgcgcggcga tttggatcga agccaacgtg aaaggggaag ttgacgccct
     1021 gcgtgcggcc agtaaaacat ttgccgacaa actggcaact tttgaagcga aattcccgga
     1081 cgcgcatctt ggtgcggtgg ttgcctttgg taacaacacc tggcgcgctc tgagcggcgg
     1141 cgttggggca gaagagctga aagattttcc gggctacggt aaaggccttg cgccgaccac
     1201 ccagttcgat gtgttgatcc acattctttc tctgcgtcac gacgtaaact tctctgtcgc
     1261 ccaggcggcg atggaagcct ttggtgactg cattgaagtg aaagaagaga tccacggctt
     1321 ccgttgggtt gaagagcgtg acctgagcgg ctttgttgac ggtacggaaa acccggcggg
     1381 tgaagagacg cgtcgcgaag tggcggttat caaagacggc gtggatgcgg gcggcagcta
     1441 tgtgtttgtc cagcgttggg aacacaacct gaagcagctc aaccggatga gcgttcacga
     1501 tcaggagatg atgatcgggc gcaccaaaga ggccaacgaa gagatcgacg gcgacgaacg
     1561 tccggaaacc tctcacctca cccgcgttga tctgaaagaa gatggcaaag ggctgaagat
     1621 tgttcgccag agcctgccgt acggcactgc cagtggcact cacggtctgt acttctgcgc
     1681 ctactgcgcg cgtctgcata acattgagca gcaactgctg agcatgtttg gcgataccga
     1741 tggtaagcgt gatgcgatgt tgcgtttcac caaaccggta accggcggct attatttcgc
     1801 accgtcgctg gacaagttga tggcgctgta agcgtttctg attcattgat taaaggccag
     1861 gcagtaacat tactgactgg cctttttatt acttttgctt taacgccgca tacacctgat
     1921 caactaccca accatacgtt gcaatcggca gctgaccgct ttcgaacata ttgggattct
     1981 tttgcggatc ggcaaccggc gaatgtggct tgttgcttaa catcacaatc gccatatgat
     2041 tcaccggatc gataacggtc accgttccgg tccagccagt gtgaccgtag gtttgcgggc
     2101 ttgccagcgt gccaaacgtc ggcgtcatgg tggcattacc attcacgcgc cagccgaggc
     2161 caaaagtggc atcttcctta gagctggtgg tgaacatctt caccgtttcc gcactgaaca
     2221 gctgcacatc gccatagccc ccgccgttca gcatcgtttg cattaacacc gcaatatcgc
     2281 cggtattgga aaacaaacct gcgtgcccgg aaacgccgcc catcgaataa aaggcttttt
     2341 catcgtgcac ctgaccccag agagtggagg tgcggatatt cgggaaatgg atcacgccat
     2401 cgcgggtatt gccgttcaat tccgtggcgg caatttgttg cggtttaaag cctttcagca
     2461 gcgggttaaa taccgtatgt gtcaggccga gcgggcgata aatcgactct tcaacatagc
     2521 ggtcaagcgg ctgaccggtg accgactcga cgataaatcc aagcagcata taatcgacat
     2581 cactgtaaat atgcttgctg ccgggctgat actctagcgg cgtgcgcttg atcatctcca
     2641 gcgtctggcc tttatcctgg gaatataacg cgcccgcgac cgctttattc gggtattgcg
     2701 gatctgccgg gaaaccgccg ctgtgatgca gcaggtcaga aatccgcagg gtgtttttgc
     2761 ctttaatggt gtcatttgga ctatcggcaa accccggaat atatttcgca atccgatcgt
     2821 caggatgcag tttgccttcg ctcatcagct tttgcagggc gaagttcgtg gcgtacattt
     2881 tggtgtttga ggccaggtca tacagcgtcc cggtggtggc tttgacaggc tgttccatca
     2941 acacgctgcc atcgtacttt ttcgccgctc cccaggcttt gcgatacaca atctgattat
     3001 ctttaatgat cagcaggttt acgccgggat aaccggcgtc cacttgctgg ctaatccagc
     3061 gatccatctg gttaagccgt tcgacgttga accctgcttt ctctggcgaa ctttctgtca
     3121 gaacagggta ttttgcggcg ctgacgctac aactaacagc cagcagagaa agatagagca
     3181 ttgtccgttt catgatatca gtccagattg acgttacggc agccaaagag cgtggtgaaa
     3241 ataaacccgc aaacccatgc caccagaatc ccgccagcat aaactgccat tgccggaagg
     3301 atgccttgtg cggaagtcat cagcggcagc gccaccagac cagacggccc aaaggcgctg
     3361 tttaagccca tcggcagacc ccaccaggct atcaggccga taaacaaacc gcccgctgcg
     3421 ccgcctaaac aggcggtaat aaacggtttc atgcggggca gggtgacgcc gtaaatcaac
     3481 ggttcaccaa cgcccagcag accgggaata atcgccccgc gtacctgact gcgtaatgca
     3541 ctgtgcggtt gcgcccgcca gtagagtgcc agcgccgcgc ccacctggcc cgcgcctgcc
     3601 attgaaagga tgggaaataa gctgttgaat ccctggctgt ccattaacgc gaggtaaaca
     3661 ggaataaagc cctgatgcac gccaaatacc acggcgatca ggaacagccc ggctaaaacc
     3721 gcacaaccga acggattact gttcaggtgc ataaacagcc atgacatacc ttcgaataac
     3781 cagccaccca gcggcataat gatcaggtac gcaagcgtag cggtgatcag cagggtgatt
     3841 aacgaggtca gcagcatgtc gagatcgtcc ggcataaagc ggcgcaccat gccttcaatg
     3901 cgggcgcagg cccacgcggc aatcagcacg ccgataatat tgccgcgcgg atcgatgggc
     3961 agaccaaaga aatcgtgaaa accggcgtag taaccggtgg ttgcagcggg gttataaccg
     4021 agcaaaaaga gtgcggcaat aattgcgcca ttgacgcccg tgccgccaaa tgcctgggcg
     4081 gcgttatagc ccaccagaat caccaggaaa gtgaacaaac ctttgctgaa caccttcata
     4141 aaattcagcg cgtcgggtag cgtgccctga gcatctgccg gaacgtgcat caccgtggca
     4201 attaacgtcg ctatccccag cagcagaccg gcggcaataa aaccggggat cagcggcgtg
     4261 aagatggtgg cgaatttggc gagaaattgt tgcacgccgg aggtttgttt ggcttttaac
     4321 tgacgtttgt tctgggcggc gatttctgcg gcatcctgta ccggtgcctc gcccagcagc
     4381 tcgctcatcg cttttgcggc acgatgcgct ttaccaggcc caaaaacgac ctgcacctgg
     4441 tcgctggtca aaatgacgcc cttcacgcct tccagcgttt tgatattggg atcaaccagt
     4501 gaactgtcat gtacacccag acgcaggcgc gtcatacagt taccacaact ggcgatattt
     4561 cccggtccgc cgacacgggt aagaatggtg ttcagaagtt cactgctgat ctctttggcc
     4621 atgggttatt ccttgtctaa aacctgacga ataaagccgc cgtgttgatc caggcgtttt
     4681 ttagcttctg cggcatcgag atttttcagc accatcacaa tggccgtttt acagttgcgc
     4741 tcgcaggcaa ttaacgccgc ttccgcttgt tcggcattac atccggtggc gtttttaacg
     4801 atattgacct ggcgcacatg cagtttttcg ttggtggcga ccacatcgac catcaggttg
     4861 ccgaacactt tgccagattt aatcatcagc ccggtggaaa gcatattgag caccagtttc
     4921 tgcgctgtgc ctgctttcat tcgcgaagaa ccagtgacca cttcggcacc tacaatcggt
     4981 gtaatggcaa actcggcggt ggtggaaacg gcactccccg gattacagga aattcccact
     5041 gtgcggcagc cgagctggcg tgcgtattcc agtccggcaa tcacatacgg cgtgcgaccg
     5101 ctggcagcaa tgccaaccac cacatcctgt gccgttaaat taatattttt cagatcgtta
     5161 acaccacctt cccggctatc ttcagcgcct tccaccgcgt gctgaatggc atattcgcct
     5221 ccagcaatca aaccaaccac cagacccggt ttcacgccgt aggtgggcgg acattcgctg
     5281 gcatccagaa tccccagacg accggatgtt cccgcaccga gataaatcag acgaccgccg
     5341 ccgctgacct gggcgtggat aacatcgatc gccgcggcga tatccggcag gacgcgctca
     5401 acggcaagcg gtacggtttt atcttcatcg ttgataatcc ggcacatttc cagcgtcgat
     5461 acgcggtcaa tttcagccga ggcggtgttc gagccttcag taatcatctt ttctagttgc
     5521 atcggtggtg accttactat ttacgatcaa ataattgcag atgtatggaa tcatatatcc
     5581 cgaattgaca gacttaaagg aatcattgag tctacactcg cagagtgtga agagcgtcat
     5641 aagaacttaa gcggaattta tgctgccagc ccaggagagt ggaagatgtt gtacctgacg
     5701 aaaataagca atgcggggag tgaattcacc gaaaacgagc aaaaaatcgc tgactttctg
     5761 caggccaacg tgagtgagct gcaatccgtt tcttcacggc agatggcgaa acagctcggc
     5821 attagccagt cgagcatagt gaaatttgcc caaaaactcg gcgcgcaagg atttactgaa
     5881 ttgcgtatgg cgttaatagg cgaatacagt gccagccggg agaaaacgaa tgccacggcg
     5941 ctgcacctgc acagttcgat taccagtgat gattcgctgg aagtgattgc ccgcaaattg
     6001 aatcgcgaaa aagagctggc gctggagcaa acctgtgcgt tgttcgacta cgcgcggctg
     6061 caaaaaatca ttgaggtcat cagtaaagca ccgtttatcc agataacggg cctgggcgga
     6121 tcggcgctgg tggggcgcga tctgtcgttc aaactgatga aaattggcta tcgcgtggcc
     6181 tgcgaagccg acacgcacgt acaagccacc gtttcccagg cgttgaaaaa aggcgatgta
     6241 caaatcgcta tctcttacag cggcagtaaa aaagagattg tcttatgcgc agaagcggca
     6301 cgaaagcagg gggcaacggt gattgccatt acttctctgg ctgattctcc gctgcggcgg
     6361 ctggcgcatt tcacgctcga taccgtttcc ggtgaaaccg aatggcgtag ctcgtcgatg
     6421 tctacccgca cggcgcaaaa ttctgttacc gatttactgt ttgttggtct tgtgcaactc
     6481 aatgatgtgg agtcattgaa aatgattcag cgcagcagtg agctaacaca acgcctgaaa
     6541 tgatgcataa agcagcgact ggattgagat tttcctgaat tagtgagctg atccgcagca
     6601 atattttgtt tatcctgtat tttcagaggg aatggagtgt aacgctctgt attaacaagg
     6661 agagcattaa aatgggtaaa ctcacgggca agacagcact gattacgggc gcattgcagg
     6721 gaattggcga aggaattgcc agaacttttg cgcgtcatgg cgcaaaccta atcttgctgg
     6781 atatctcccc tgagatcgaa aagctggcgg acgaactgtg tggtcgtggt catcgctgta
     6841 cggcggttgt cgccgatgtg cgtgacccgg cgtcggtagc cgcagctatc aaacgcgcga
     6901 aggaaaaaga agggcgcatt gatatcctgg tgaataacgc aggcgtttgt cgtctgggca
     6961 gtttcctcga tatgagcgat gacgatcgcg atttccatat tgacatcaat attaaaggcg
     7021 tatggaacgt cacgaaggcg gtgctgccgg agatgattgc ccgcaaagat ggtcgcattg
     7081 tgatgatgtc ttcggtcact ggcgatatgg tggccgatcc tggcgaaacg gcgtacgcct
     7141 taacgaaagc ggcgattgtt ggcctgacaa aatcgctggc ggtggagtac gcgcagtctg
     7201 gtattcgcgt taacgcc
//
LOCUS       DAMZET010000192         7214 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.192, whole genome shotgun sequence.
ACCESSION   DAMZET010000192 DAMZET010000000
VERSION     DAMZET010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.192"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            405..1487
                     /locus_tag="POB34_004126"
     CDS             405..1487
                     /locus_tag="POB34_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HDI5920981.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRNEVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNGLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEA
                     VAQYQFDFGLRPSVAYLHSKGKDLGVWGDQDLVEYVDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
     gene            complement(1676..1894)
                     /gene="quuD"
                     /locus_tag="POB34_004127"
                     /pseudo
     CDS             complement(1676..1894)
                     /gene="quuD"
                     /locus_tag="POB34_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
     gene            2003..2203
                     /locus_tag="POB34_004128"
     CDS             2003..2203
                     /locus_tag="POB34_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction Ral family protein"
                     /protein_id="HDI5920982.1"
                     /translation="MTTTIDKNQWCGQFKRCNGCKLQSECMVKPEEMFPVMDDGKYVD
                     KWAIRTTAMIARELGKQNNKAA"
     gene            2386..2754
                     /locus_tag="POB34_004129"
     CDS             2386..2754
                     /locus_tag="POB34_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HDI5920983.1"
                     /translation="MSNIKKYIIDYDWKASIEIEIDHDVMTEEKLHQINNFWSDSEYR
                     LNKHGSVLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIR
                     ITDIDTSGIFDPDDMTIKAA"
     gene            2827..2991
                     /locus_tag="POB34_004130"
     CDS             2827..2991
                     /locus_tag="POB34_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HDI5920984.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNTYGYPD"
     gene            2960..3112
                     /gene="kil"
                     /locus_tag="POB34_004131"
     CDS             2960..3112
                     /gene="kil"
                     /locus_tag="POB34_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HDI5920985.1"
                     /translation="MDQTLMAIQTKFTIATFIGDEKMFREAVDAYKKWILILKLRSSK
                     SIHYPP"
     gene            3102..3305
                     /locus_tag="POB34_004132"
                     /pseudo
     CDS             3102..3305
                     /locus_tag="POB34_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3302..3982
                     /locus_tag="POB34_004133"
     CDS             3302..3982
                     /locus_tag="POB34_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HDI5920986.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNITE
                     SPIIYRDENMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            3979..4164
                     /locus_tag="POB34_004134"
     CDS             3979..4164
                     /locus_tag="POB34_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HDI5920987.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKWGGAVCTKHLPLS"
     gene            4137..4328
                     /locus_tag="POB34_004135"
     CDS             4137..4328
                     /locus_tag="POB34_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HDI5920988.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLAASL
                     SQKLEMMVAKAEADERDQV"
     gene            4405..4620
                     /locus_tag="POB34_004136"
     CDS             4405..4620
                     /locus_tag="POB34_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDI5920989.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            4719..4940
                     /locus_tag="POB34_004137"
     CDS             4719..4940
                     /locus_tag="POB34_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HDI5920990.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLVVQGCRTCASCQEDLELISKQRGSK"
     gene            4937..5344
                     /locus_tag="POB34_004138"
     CDS             4937..5344
                     /locus_tag="POB34_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HDI5920991.1"
                     /translation="MSEINSQALREAAEKAGEDKWQAKKINGDFFVIRHGSYTRQHGY
                     TSNQPIAEIDCKPVRDFVAKANPATVLELLDELEAAKKRIAELEAREILLPERSSMLH
                     RTDFHDDYQTVMAYKVSEVIDAIRATGIRIKGE"
     gene            5346..6101
                     /locus_tag="POB34_004139"
     CDS             5346..6101
                     /locus_tag="POB34_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5920992.1"
                     /translation="MIHYHGGPITPDTCAMRAWKGRHAFISFAHSGQINLAAEYCQSF
                     ALDNGAFTAWKAAGKNKIDWSDYYEFVARWKNHPGFDFAIIPDIIDGGEEENDALLNE
                     WPHGKLAGVPVWHMNESDARFIHLCNEFPRVAIGSCGDYDVKRPTLAVARMKDLIRHI
                     VDGHGQPVTKLHGLRMLNPLIFTKLPLASADSTNVARNIGIDKAWSGAYAPASKETRA
                     ALMVERIEAHNSPGSLAYCEQRDRFEMQLQLAV"
     gene            6112..6909
                     /locus_tag="POB34_004140"
     CDS             6112..6909
                     /locus_tag="POB34_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF550 domain-containing protein"
                     /protein_id="HDI5920993.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLERELIRH
                     EHAKWSDSTFGCVGPIGPLKHLSKEALEAAAEPDDLSEWADMHFLLWDAQRRAGISDA
                     EITAAMEDKLKINMERQWPEPKDGEPRLHIKEPGNSPVIPDGLSTVCAEAYQVVGVMA
                     DALGVFGDAAVQKVLDNLSQQKLVHRDVLPFSLPVTPDGWISCSERMPKETGDIIVVS
                     DGIVMSGISYSRRDGFYIAALEYDDDEPIGGVTHWMPLPEPPRLKEL"
BASE COUNT         2153 a         1534 c         1676 g         1851 t
ORIGIN      
        1 cttaacagca taaccaattt tttacgtacg taaaccacta aatgatattt gcgagaatgc
       61 taccgagtat tgaaaacacc actacaaata cataagaaaa tctcaacaaa taaccaataa
      121 ataatttcca gtgttatttt tagccggttt aaattgaacc ttcaaattat agagcactta
      181 taaataacag ccgttaatat aaattggcta atagatttat ttttattcag ccaagagcca
      241 tgaataggat tcgatagaaa aaagttcaga taaaaataga gatctacttc acaaatcaaa
      301 cgagaaacta aaacttacat cttgaaataa tcacattgat tagatgaata tttatcgcgc
      361 agtgacatca ttttttaata atagttcaaa aaaaagggct cacgatgaaa aaattaacag
      421 tggcaatttc tgctgtagct gcatcagtac tgatggcgat gtctgctcag gcagctgaaa
      481 tttataataa agacagtaac aagctggatc tgtacgggaa agttaatgcc aagcactact
      541 tctcctctaa tgatgcagat gatggtgata ctacttatgc ccgtcttggc ttcaaaggtg
      601 aaacccaaat caacgatcaa ctgactggtt tcggtcagtg ggaatatgaa ttcaaaggca
      661 accgcgctga atctcaaggt tcctccaaag ataaaacccg tcttgccttc gctggcctga
      721 aattcggtga ctacggctcc atcgattacg gccgtaacta cggtgtagca tacgacatcg
      781 gtgcatggac cgacgttctg ccagaattcg gtggcgatac ctggacccaa acagatgtat
      841 tcatgactgg tcgcaccaca ggtgttgcaa cttatcgtaa caacgacttc tttggtctgg
      901 ttgatggcct gaactttgca gctcagtatc agggcaaaaa tgaccgaaat gaagtaactg
      961 aagctaatgg cgatggtttc ggtttctcaa ctacttatga gtatgaagga ttcggcgtgg
     1021 gtgcaaccta tgctaaatct gatcgcacta ataatcaggt tatctacggt aacaacggtc
     1081 tgaatgcttc tggtcaaaat gctgaagtat gggcagctgg tctgaaatat gatgcgaaca
     1141 acatctatct ggccaccacc tattctgaaa cccagaacat gactgttttt ggtaataacc
     1201 atattgccaa caaagcacaa aacttcgaag ctgttgcaca atatcagttc gacttcggcc
     1261 tgcgtccatc cgttgcttac ctgcattcta aaggaaaaga cttgggtgtt tggggtgatc
     1321 aggacctggt tgaatatgtt gatgtaggtg caacctatta cttcaacaaa aatatgtcca
     1381 cttttgttga ctacaaaatc aacctgattg ataagagcga tttcacgaaa gcatctggcg
     1441 ttgctaccga tgatatcgtt gctgtaggta tggtttacca gttctaattt gattactaaa
     1501 agatatgttg tgggaggctt tgcctcccca acatataagt ggctccctca agccacttcc
     1561 tttagaagca caaccttgct tctaactata taaaccttct gttatatatt accctttatt
     1621 tttgggggcg tttcaacgcc ccatttttaa taacttttag taaataattg gcgtattaat
     1681 tagagttatt aacaacgata tccatctcta accggatatc taatgccatt aacatccctt
     1741 caattatgcc ctcagccttt tgtaaccttt tcccgatata accatcagag cagcaatgct
     1801 tacctgccag tgacatgaat gtcataccga ctatataata atctactaat aaatcgtgca
     1861 aatcgctgtt gttctttttc agacgggcca tgcacaatct ttactaccgc aatccactat
     1921 ttaaggtgat acatggaaga agaatttgaa gagttcgaag agcatcctca ggatgtgatg
     1981 gaacaatacc aggactatcc gtatgactac gactattgat aaaaatcaat ggtgtggaca
     2041 attcaagcga tgcaatggat gcaagctgca atcggaatgc atggttaagc ctgaagaaat
     2101 gtttcctgta atggatgatg ggaaatatgt cgataaatgg gcaatacgaa cgacggcaat
     2161 gattgccaga gaacttggta aacagaacaa caaagctgcc tgatagtggc ctttattttt
     2221 ggcataaata acagaataaa cactgcactg tgtattcatt ccaacgagtg aatacacgga
     2281 gcaatgtcgc tcgtaactaa acaggagccg acttgttctg attattggaa atcttctttg
     2341 ccctccagtg tgagggcgat tttttatcta tgaggatatg aatagatgtc aaacatcaaa
     2401 aaatacatca ttgattacga ctggaaagca tcaatagaaa ttgaaatcga tcatgacgta
     2461 atgacagagg aaaaacttca ccagattaat aatttctggt cagactctga ataccgactc
     2521 aataaacacg gctctgtatt aaatgctgta ttaatcatgc tggcgcaaca tgctctgctt
     2581 atagcaattt caagcgactt aaatgcatat ggtgttgtgt gtgagttcga ctggaatgat
     2641 ggaaatggtc aggaaggatg gcctccaatg gatggtagcg aaggaataag aattaccgat
     2701 atcgatacat caggaatatt tgatccagat gatatgacta tcaaagccgc ctgagcgcgg
     2761 cgttaccgca taccaataac gcttcactcg aggcgttttt cgttatgtat aaataaggag
     2821 cacaccatgc aatatgccat tgcagggtgg cctgttgctg gctgcccttc cgaatcttta
     2881 cttgaacgaa tcacccgtaa attacgtgac ggatggaaac gccttatcga catacttaat
     2941 cagccaggag tcccaaaaaa tggatcaaac acttatggct atccagacta aattcactat
     3001 cgccactttt attggcgatg aaaagatgtt tcgtgaggcc gtcgacgctt ataaaaaatg
     3061 gatattaata ctgaaactga gatcaagcaa aagcattcac tacccccctt aacgatgaaa
     3121 ccatgcagga gattaacact ctgctgatcg ccctggataa aacatgggat gacgacttat
     3181 tgccgctctg ttcccagata tttcgccgcg acattcgcgc atcgtcagaa ctgacacagg
     3241 ccgaagcagt gaaagctctt ggattcctta aacagaaagc cactgagcag aaggtggcag
     3301 catgacaccg gacattatcc tgcagcgtac cgggatcgac gtgagagctg tcgaacaggg
     3361 ggatgatgca tggcacaaat tacggctcgg cgtcatcacc gcttcagaag ttcacaacgt
     3421 gatagcaaag ccccgctcag gaaagaagtg gcctgacatg aaaatgtcct acttccacac
     3481 cctgctggct gaggtttgca ccggtgtggc tccggaagtt aatgctaagg cgctggcctg
     3541 gggaaaacag tacgagaacg acgccagaac cctgtttgaa ttcacttccg gcgtgaatat
     3601 tactgaatcc ccgatcatct atcgcgacga aaatatgcgc accgcctgct ctcccgatgg
     3661 tttatgcagt gacggcaacg gccttgaact gaaatgcccg tttacctccc gggatttcat
     3721 gaaattccgg ctcggtggtt tcgaggcaat aaaatcggct tacatggccc aggtgcagta
     3781 cagcatgtgg gtgacgcgaa aagatgcctg gtactttgcc aactatgacc cgcgcatgaa
     3841 gcgtgaaggc ctgcattatg tcgtgattga gcggaatgaa aagtacatgg cgagttttga
     3901 cgagatggtg ccggagttca tcgaaaaaat ggacgaggca ctggctgaaa ttggttttgt
     3961 atttggggag caatggcgat gacgcatcct cacgataata tccgggtagg cgcaatcact
     4021 ttcgtctact ccgttacaaa gcgaggctgg gtatttcccg gcctttctgt aatccgaaat
     4081 cccctgaaag cacagcggct ggctgaggag ataaataata aatggggggg ggctgtatgc
     4141 acaaagcatc tcccgttgag ttaagaacga gtatcgagat ggcacatagc ctcgctcaaa
     4201 ttggagtcag gtttgtgcca ataccagtag aaacagacga agaatttcac acgttagccg
     4261 catccctttc acaaaagctg gaaatgatgg tggcgaaagc agaagcagat gagagagacc
     4321 aggtatgaca accactgaat gcatttttct ggcagcgggc ttcatattct gtgtgcttat
     4381 gcttgccgac atgggacttg ttcaatgaca cctcagcagg aaaacgccct tcgcagcatt
     4441 gcccgtcagg ctaattctga aatcaaaaaa gccagacagc agtttccgga taaaaacgtc
     4501 gatgacattt gccgtagcgt actgaagaag caccgcgaaa cggtaacgct gatgggattc
     4561 acaccgactc atttaagcct ggcaatcggc atgttaaacg gcgtctttaa ggaacgatga
     4621 acatgaaaag caaaatcatc agggagctac aggctccttt tttattattc gcattcaccc
     4681 tcaagcgtat taaccaacaa ttcagggatt aatggaagat ggcagacatc attgattcag
     4741 catcagaaat tgaagaatta cagcgcaaca cagcaataaa aatgcgtcgt ctgaactacc
     4801 agactgtatc tgccactcat tgttgtgagt gtggcgatcc gatagatgaa cgaagacgcc
     4861 tggtcgttca gggttgtcgg acttgtgcaa gttgccagga ggatctggag cttatcagta
     4921 aacagagagg ttcgaagtga gcgaaattaa ctctcaggca ctgcgtgaag cggcagagaa
     4981 agccggtgaa gataagtggc aggctaaaaa aataaatggt gattttttcg ttattcgtca
     5041 cggtagttat acaagacagc atggctacac atcgaatcaa cccattgcgg agattgattg
     5101 taagccagtc cgggattttg ttgccaaggc taatccggct accgtgctgg aattactgga
     5161 tgaactggaa gcagcaaaaa agcgcattgc agaactggaa gcgcgggaaa tactgctccc
     5221 ggaacgtagc agcatgcttc atcgaacaga ttttcacgat gattaccaaa cggtaatggc
     5281 atacaaagtt tctgaagtca tcgatgcaat ccgcgctact ggcattcgca tcaaaggaga
     5341 gtgagatgat tcactaccac ggtgggccta ttactcctga tacgtgcgca atgagagcat
     5401 ggaaagggcg acatgcgttt atcagttttg cgcattcagg ccagatcaat ctcgcggctg
     5461 aatactgtca gtcgttcgcg ctggacaacg gtgcattcac cgcctggaaa gcagctggca
     5521 aaaacaaaat cgactggagc gattactacg agtttgttgc tcgctggaag aatcacccag
     5581 gattcgattt tgccattatc ccggatatta ttgatggcgg agaggaggaa aatgatgcgc
     5641 ttctgaatga gtggcctcac ggaaaactag ctggcgttcc agtgtggcac atgaatgaaa
     5701 gtgacgcgcg atttattcat ttgtgcaatg agtttccgcg agtggctatc ggtagttgtg
     5761 gcgactatga cgtaaagcgc ccaactcttg cggtagccag aatgaaagac ctgattcgtc
     5821 acattgttga tgggcatggt cagccggtta cgaaactaca tggattgcgc atgttaaatc
     5881 cgctgatatt cacaaaatta cccttagcca gcgcagatag tacgaacgtc gctcgaaaca
     5941 tcggtattga taaagcctgg tctggggctt atgcacctgc aagtaaagag acacgcgcag
     6001 cattaatggt agaacggatt gaggcacaca atagccctgg ttctcttgcg tattgtgaac
     6061 aacgcgaccg ctttgaaatg caattgcaac tagcagttta aggactaaca aatgaccact
     6121 attaccaaag aacgtattga attgttcatt aaaaatccgc ttgaaaacgg gcttactcgt
     6181 ggcgaacaaa tggaactggc acgaattgca ctggcatcac tggaacgcga actgattcgc
     6241 cacgagcatg ccaaatggtc tgactccaca tttggctgcg ttggccccat tggtccactg
     6301 aaacacctct caaaagaggc tctggaagcc gcagccgaac cagacgatct cagcgagtgg
     6361 gctgatatgc atttcctgtt gtgggatgca cagcgccgtg ctggcatcag cgatgctgaa
     6421 attaccgctg ctatggaaga taaattgaag atcaacatgg agcgccagtg gcctgaacca
     6481 aaagatggtg agcctcgctt gcacattaaa gaacccggca actctccggt aattccggat
     6541 ggtttatcca cggtatgcgc tgaggcttat caggttgtag gagttatggc agatgcgctt
     6601 ggtgtattcg gtgatgcagc agtacagaaa gttctggata acctgtcaca gcaaaaactt
     6661 gttcacagag atgtgctgcc gttctcgctt ccggtgactc cggatggttg gataagctgt
     6721 agtgagcgaa tgccgaaaga aacgggtgac attattgttg tttcggatgg cattgtaatg
     6781 tccgggattt cttattctcg tcgtgacggg ttctatatag ccgcattgga gtacgacgac
     6841 gatgagccaa ttggcggtgt aacccactgg atgcctctac cagaaccgcc tcgattaaag
     6901 gagctataat agtgaactat tatatctatt tgtattaaaa gagtttttat aaaataaatc
     6961 tcccaaagca tgtaaaaaca ctgttaatct taacgtgtgt gaaacgtgaa gagaggtgtt
     7021 gaaatgagca ttcatgattt gtgtgaagat caagagcaat gggctatgca gacccttatg
     7081 ggatcaggag ttcttgcaag gtgcagaatc cataacgatg taattttaga cagcggaaat
     7141 gatgcttctt ctgcttataa attaggaact tacctatatc aaaaagataa tagctgcaac
     7201 ttattcaata ctct
//
LOCUS       DAMZET010000193         7214 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.193, whole genome shotgun sequence.
ACCESSION   DAMZET010000193 DAMZET010000000
VERSION     DAMZET010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.193"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1117)
                     /locus_tag="POB34_004141"
     CDS             complement(<1..1117)
                     /locus_tag="POB34_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HDI5920994.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGERMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQ"
     gene            complement(1180..1893)
                     /gene="ydiV"
                     /locus_tag="POB34_004142"
     CDS             complement(1180..1893)
                     /gene="ydiV"
                     /locus_tag="POB34_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HDI5920995.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            complement(2140..2604)
                     /locus_tag="POB34_004143"
     CDS             complement(2140..2604)
                     /locus_tag="POB34_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HDI5920996.1"
                     /translation="MRFCLILITALLLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            complement(2682..3431)
                     /gene="btuD"
                     /locus_tag="POB34_004144"
     CDS             complement(2682..3431)
                     /gene="btuD"
                     /locus_tag="POB34_004144"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HDI5920997.1"
                     /translation="MSIVMQLQDVAETTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            complement(3431..3982)
                     /gene="btuE"
                     /locus_tag="POB34_004145"
     CDS             complement(3431..3982)
                     /gene="btuE"
                     /locus_tag="POB34_004145"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HDI5920998.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            complement(4045..5025)
                     /gene="btuC"
                     /locus_tag="POB34_004146"
     CDS             complement(4045..5025)
                     /gene="btuC"
                     /locus_tag="POB34_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HDI5920999.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            complement(5126..5425)
                     /gene="ihfA"
                     /locus_tag="POB34_004147"
     CDS             complement(5126..5425)
                     /gene="ihfA"
                     /locus_tag="POB34_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HDI5921000.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(5430..>7214)
                     /gene="pheT"
                     /locus_tag="POB34_004148"
     CDS             complement(5430..>7214)
                     /gene="pheT"
                     /locus_tag="POB34_004148"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDI5921001.1"
                     /translation="TLPITVEAPEACPRYLGRVVKGINVKAPTPLWMKEKLRRCGIRS
                     IDAVVDVTNYVLLELGQPMHAFDKDRIEGGIVVRMAKEGETLVLLDGTEAKLNADTLV
                     IADHNKALAMGGIFGGEHSGVNDETQNVLLECAFFSPLSITGRARRHGLHTDASHRYE
                     RGVDPALQHKAMERATRLLIDICGGEAGPVIDITNEATLPKRATITLRRSKLDRLIGH
                     HIADEQVTDILRRLGCEVTEGKDEWQAVAPSWRFDMEIEEDLVEEVARVYGYNNIPDE
                     PVQASLIMGTHREADLSLKRVKTLLNDKGYQEVITYSFVDPKVQQMIHPGVEALLLPS
                     PISVEMSAMRLSLWTGLLATVVYNQNRQQNRVRIFESGLRFVPDTQAPLGIRQDLMLA
                     GVICGNRYEEHWNLAKETVDFYDLKGDLESVLDLTGKLNEVEFRAEANPALHPGQSAA
                     IYLKGERIGFVGVVHPELERKLDLNGRTLVFELEWNKLADRVVPQAREISRFPANRRD
                     IAVVVAENVPAADILSECKKVGVNQVVGVNLFDVYRGKGVAEGYKSLAISLILQDTSR
                     TLEEEEIAATVAKCVEALKERFQASLRD"
BASE COUNT         1820 a         1974 c         1699 g         1721 t
ORIGIN      
        1 gctgctcggt cagactcagc atgcggaatg tgcgggtata atcgctgcgc tctcgcgcca
       61 tcagactgaa taattcattc agtagcgcgt tatcctcttt ttgctccgtc atgaagccca
      121 gtttctgccg catccgttct ccataatgcg tcaacaaaac ctgctgatag ctgtccaggg
      181 cctcattcag ggcatctacg gcaacaaatg gcgacaatgt ctgcgccaga cgctgtaaat
      241 tccacaacgc gacggcaggt tgattatcaa agctgtaacg cccttgatga tccgagtgat
      301 tacaaataaa accgggttcg taatcatcaa gaaaaccaaa cggcccgtaa tcaagcgtca
      361 gccccagcag cgacatgttg tcggtattca tcaccccatg agcaaagccg accgtctgcc
      421 attgggcaat taacgacgcg gtacgtgcga caacatcgct aaaccagaga cggtatttgt
      481 cctcatcatc ttcaagatgt gaccagtaat gacgaatagc aaagtcagct aactgacgaa
      541 ccttttccga ctcgcggcgg tagtaaaaat gttcgaaatg accaaagcgc agatgacttg
      601 gtgccacacg catcagcatc gcgcctggct ccgccgtttc ccgatacact ggcgaatcgc
      661 tggtgacgat acttaacgcg cgggtcgtcg gaatgcccag ataatgcatc gcctcactgg
      721 cgagactttc tcgtatcgtc gaacgtaaaa ccgcccgtcc atcacccatt cgcgaataag
      781 gcgtcaggcc agcacctttc agatgccagt ccattgtagt gccatcagca agcagttgtt
      841 cgccgagtaa aatgccgcgc ccatcaccca gttggcccgc ccagacgccg aactgatgac
      901 cactgtaaac ctgcgccagt ggtgacatac caggcagtag ggcttcgccg ccccagacac
      961 ctgcgccatt tttaaacagc gacgatggaa tactcagcgt gttagccagt tcggtattat
     1021 gccaaatcag ccgggcatta tttaaaggcg taggggaaag tgctgtatag gtttctggca
     1081 attcatcgcg ccagcgggta acaaaagaca gggtcataga tcctcctgtt tgatagtgta
     1141 gacggttact ctcgtcttaa acaccagcaa acagaagggt tatcgctgaa ccaacgtcgt
     1201 tatctgattt atgggaacgg caggccacag gcatccctga agagcatgga aatcgaaggg
     1261 ggtaatctgt gccaatattt ctgccgtatc aatgcctccc gcaatgatgc aattgcaaca
     1321 aggggaaatt tgcgcctgga tcgcacgaat aaacggttcg aaggagcggt gcgtgatttg
     1381 ttgctgaata aaacttttat ccagcatgac gcgggtgaaa agaccatcaa aaaccgcttt
     1441 cattgtgcta ttgcctgctc ccaaattacc taacacaagg ggatacactt gcgataagga
     1501 taaaagaccg cgattatctt ttccttcatt gagatgcggg taattctcat taatcaacag
     1561 ttcaataaac ggatatttta atagttcgcc tgcgtaatta tccctctcta ataataatgt
     1621 agccacttgt ggcgttaaat taagccaggc aaagagttta tgttgaataa aaaaatgttg
     1681 gcatgatttt agtaattcca actgctctga aaataattgc caatgttgct cctcggtaag
     1741 ttgcgctata acccgactcg tcgggatacg caccgtgcca tcttcactgg agaaatgggt
     1801 aattaactca acgccgacaa ggacttgctg gttgtctctt atcggcagga aatagcaatc
     1861 agaatgataa agattctcca aaaaaatctt catggtaacc gtccctctcg aaggatgttt
     1921 ttcagtatcc ggctaaaagg gatgaggcca taagacataa taattacaga aggagtaact
     1981 ttcatttgtt ccatgttaac cactttttca ggggtccttt ttttagatta tcctgattat
     2041 aaacgaataa tccatttacg gaatttttgt ctgcaaaata ctactgtatt caggagtaaa
     2101 tggacggcga tgtatcatcg ccgtccattt ttcgacatac tagattcgtc tcgcctgcca
     2161 gaaatttttc tgccaataga cattatcaag tgaggaacgc atcactccct tgctggtaga
     2221 ggcgtggata aattggttgt tggtatcgta aatacctaca tgcaaaccat tttgtccgga
     2281 ccccgttttg aaaaagacca ggtcacctgg cagcaactcg tctttatcaa tttgcgtgcc
     2341 aatagaggct tgttgtttgg tttcacgggg cagctgcaaa tcgaaacgat cgcgcatcgt
     2401 cacaaccaca aatcccgaac agtccacacc gcgccgcgtc atgccaccat aacgatacgg
     2461 cgtgccatgc cagctttgta gctggtcgtt caaaccggca ataacggtaa tcgaatcaga
     2521 aagtctggca tttggcggcg gtgctttatg gtggctacac ccggccagaa gcagtgctgt
     2581 gatcaaaata aggcagaaac gcattccgta cggttcctct gttttttatt cttgcattaa
     2641 tttagcgtcg taattacccg attttcaaga tactaatgaa atcagatggt cgaaatcagc
     2701 attctgtgac cttcgatatc cagacggcga aaattcatcc cataggcctg cgccagattt
     2761 ggcggcgtga gcacctcttc cctgcgtcca ctggccagca tttttccacc ttttagcaac
     2821 cacgcccgat gcgcatgacg caatgtgtgg ttgagatcgt gactgctcat cacaatcgcc
     2881 agtccttgct gacacagcgc gctcagaatt ttgtctaacg cactttgttg cgcaacatca
     2941 agactgttca tcggctcatc aagaagcagc aattggcctg cgggattggc ttgtggtgtg
     3001 atttgcaaca ccaccgcagc aagacgtacg cgttgccatt caccgccgga aagttgattg
     3061 gtgctacgtc cgagtttgtc atcaagagcc agcgcccctg cgacatcatt cagtagttcg
     3121 gtacgcgttt tatcgtgctg atgcagtgtc aggtagtgcc agaccggcat tgcaaacggc
     3181 ggcgtctgct gttgtgaaag ataggcgcga tgcagcgcga gttttgttgc ggaccatgct
     3241 tccagtggtt gccccgcgaa ctgaatgctt cccttaccgc tggtcattcc ggccattcgc
     3301 gccagtaagg tactcttacc cgcgccattc ggccccacca ggtgcaggat ctccccagcc
     3361 cgaacctcgc cagaaagcgg ccccaggcgg gtagtttccg caacatcctg taactgcatc
     3421 acaatagaca ttattttgcc aacgccagtt taatgctttc catcacaatg ggatcttcag
     3481 gcgtcatatc cggggaaaaa cgctggatga ccaacccgtc cctgccaact aaaaatttct
     3541 caaaattcca taaaatatca tcagggtaca gcggtgcacg acctttgctg accatacggg
     3601 catagaatcc gctctcttcc ggcgcgactg cggtcggcgc tgcggcaatc aatttttgat
     3661 acagcggatg gcgtccttcg ccattaactt caatcttact gaacatcggg aacgtcaccc
     3721 cccatgtggt ggtacagtaa gttttaatct cttcatcgct gcccggttct tgttccagaa
     3781 actggttgca cgggaatccc agcaccacaa aacctcgatc ggcccaggct ttctgaatat
     3841 tctccaactg ctcatattgc ggcgttaagc cacactttga ggcgacattg acaatcaaca
     3901 gcacattacc agcgtacttc tccagcgtgg tcacttcacc gtcgatatct ttcactacgg
     3961 tcgtcagaat ggaatcttgc atcgtttctc ctgggtgtgg tcagtaaaaa tcttagcttt
     4021 taatcataga ccgtcttttt gcggctaacg tcttgctttt aacaataacc agataaacac
     4081 cggcgcaccc aacgttgcgg tgaccacgcc aataggcagc tctgcggcag ctaatgccag
     4141 gcgcgctaca atatcggcca gcagcaatgc gctcgcccct gccagcgcgc agccgggaag
     4201 taatacgcga tgatcggtta aaccacacaa ccgaagaata tgtgggatca ccagaccaat
     4261 aaagccgata gcacccgcca gcgccacact gacgccaacc atccagccgg tcgcggccac
     4321 cagcacattg cgccagaacc acaggggtaa acccagttgc cgcgccgaga tctcgccaag
     4381 tgctaacata ttcatcggcc tggactgaca acagatccac aacaacacgg ggatcaatgc
     4441 cagcatcagc cagctttgcc gccagtctac gccgccaaaa ccgcccatca tccagtacat
     4501 cagctgacgt aaatcaacag aggtggaaaa gtagatagcc cacgtcatta gtgcgctaca
     4561 gataatccct aatgcaacgc cagccagcaa taaccgactg gtcgaaagat gacgacgggc
     4621 gaaacgtaag agtattaaag tgatgataag cgcgccagca atcgcacaca gccctagcgc
     4681 ccagttgggg agttgccctt gcccaagcaa taccgcggcg ataagcccca cgcctgcgcc
     4741 gttagagacg ccaagtagtc caggttctgc cagaggattt tcaaacaacg cctgcattac
     4801 agcgccggat atagccagcg ccgcaccaac cagcaataca gccagcgtac gtggcaggcg
     4861 aatttgccag acgaacagtt cgccacgagg agtaaaccag tcacctggcg agatccattg
     4921 ttcaccggcg caaaggctta agagaagcgc cagcagcatc aaaactgaca ggcataataa
     4981 ccagcgaata ttttgtcgct gttgttggcg ggcaagtgtc agcatggtat ccgttctgct
     5041 gaagtgtcat ggcgttgatt ttacggtgac tcttcgacag tgaaaagaaa aaaggccgca
     5101 gagcggcctt tttagttaga tcagattact cgtctttggg cgaagcgttt tcgacccggc
     5161 tttttaactt ctgcccgggt ctgaaggtca ccacgcgccg tgctgtaatg ggaatatcct
     5221 cgcccgtttt cgggttacgt cccgggcgtt gattcttatc acgcagatcg aagttaccaa
     5281 aaccagagag tttcacctgt tcgccgtttt ccagagcgcg acggatctct tcgaaaaaca
     5341 gttcaaccag ttctttggca tcccgcttgc taagcccaag cttatcaaac agatattctg
     5401 acatttcagc ttttgtaagc gccataggtt caatccctca atgatgcctg gaatcgctct
     5461 tttaatgcct ctacacattt ggcgacggta gcggcaatct cctcttcttc gagtgtacgg
     5521 ctggtatctt gcaggatcag gcttatggcg aggctcttat acccctccgc aacaccctta
     5581 ccgcggtaca cgtcaaataa gtttacgcca actacctgat ttacgccaac tttcttacat
     5641 tcggataaaa tatccgctgc gggaacgttt tctgcgacca ccaccgcgat gtcacgacgg
     5701 ttcgccggga agcgagaaat ctcgcgcgcc tgaggcacca cgcggtctgc gagcttgttc
     5761 cactccagtt cgaacaccag agtgcgaccg ttaagatcca gtttacgttc cagttcagga
     5821 tgaacaaccc caacaaaacc aatacgttca cctttcagat aaatcgctgc ggattgcccc
     5881 ggatgcagtg ccggattcgc ttctgcacgg aactcaacct cattcagttt accggtcagg
     5941 tcgagaacgg attcaagatc gcctttcaaa tcatagaaat caacggtctc ttttgccagg
     6001 ttccagtgct cttcgtaacg gttaccgcaa atcacaccgg ctaacatcag atcctgacga
     6061 atgcccaacg gtgcctgagt atctggtacg aaacgcagac cgctttcgaa aatgcgcaca
     6121 cggttctgct gacggttctg gttgtacacc acggttgcca gcaggccggt ccacagagaa
     6181 agacgcattg ctgacatttc aacagagatc gggcttggca gcagtaaggc ttcaacgcct
     6241 ggatggatca tctgctgcac tttcggatca acgaagctgt aggtgatcac ttcctgatag
     6301 cctttgtcgt tgagcagcgt tttcacgcgc ttgagcgaca ggtcagcttc acggtgagta
     6361 cccataatca ggcttgcctg taccggctca tccgggatgt tgttgtagcc gtaaacacgc
     6421 gcgacttctt cgaccagatc ttcttcaatc tccatatcga aacgccagct cggcgcaact
     6481 gcctgccact cgtctttgcc ttcggtcact tcgcagccga gacgacgcag aatgtcagtt
     6541 acctgctcat ccgcaatatg atggccgatc aggcgatcca gtttgctacg acgtaaagtg
     6601 atggttgcac gcttcggcag cgttgcttcg ttggtgatat caattaccgg accagcctca
     6661 ccaccgcaga tgtcgatcag cagacgggtc gcacgttcca tcgctttgtg ctgcagtgcc
     6721 ggatcaacgc cacgctcata acggtgagac gcatcagtat gcaggccatg acgacgagca
     6781 cgaccggtga tagacagcgg gctgaagaaa gcgcattcca gcaagacgtt ttgcgtttcg
     6841 tcattcacgc cagagtgttc gccaccaaag atgccgccca tcgccagcgc cttgttgtgg
     6901 tcggcgatga ccagagtgtc agcattcagc ttcgcttcag tgccatcaag cagtaccaac
     6961 gtttcgccct ctttcgccat ccgcaccaca atgccgcctt caatgcgatc tttatcgaaa
     7021 gcgtgcatcg gctggcccag ttcgagcagt acatagttgg tgacgtcaac aactgcatcg
     7081 atagaacgga tcccgcaacg acgcagtttt tctttcatcc acagcggagt tggcgcttta
     7141 acgttaatgc cttttaccac acggccaaga taacgcgggc aggcttccgg cgcttcgact
     7201 gtaatcggca gcgt
//
LOCUS       DAMZET010000194         7159 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.194, whole genome shotgun sequence.
ACCESSION   DAMZET010000194 DAMZET010000000
VERSION     DAMZET010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.194"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..755)
                     /gene="menC"
                     /locus_tag="POB34_004149"
     CDS             complement(<1..755)
                     /gene="menC"
                     /locus_tag="POB34_004149"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDI5921002.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMTSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAA"
     gene            complement(755..1612)
                     /gene="menB"
                     /locus_tag="POB34_004150"
     CDS             complement(755..1612)
                     /gene="menB"
                     /locus_tag="POB34_004150"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDI5921003.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(1627..2385)
                     /gene="menH"
                     /locus_tag="POB34_004151"
     CDS             complement(1627..2385)
                     /gene="menH"
                     /locus_tag="POB34_004151"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDI5921004.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLTG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFCTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVVLRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(2382..4052)
                     /gene="menD"
                     /locus_tag="POB34_004152"
     CDS             complement(2382..4052)
                     /gene="menD"
                     /locus_tag="POB34_004152"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDI5921005.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(4141..5436)
                     /gene="menF"
                     /locus_tag="POB34_004153"
     CDS             complement(4141..5436)
                     /gene="menF"
                     /locus_tag="POB34_004153"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HDI5921006.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(5515..5820)
                     /gene="elaB"
                     /locus_tag="POB34_004154"
     CDS             complement(5515..5820)
                     /gene="elaB"
                     /locus_tag="POB34_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HDI5921007.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDTADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(5875..6336)
                     /locus_tag="POB34_004155"
     CDS             complement(5875..6336)
                     /locus_tag="POB34_004155"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5921008.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            6401..>7159
                     /gene="rbn"
                     /locus_tag="POB34_004156"
     CDS             6401..>7159
                     /gene="rbn"
                     /locus_tag="POB34_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HDI5921009.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQ"
BASE COUNT         1625 a         2062 c         1827 g         1645 t
ORIGIN      
        1 gccgcctgta cctgctcgcg tactttctcc agactgcccg tgagcgtggg tttgataact
       61 accgcgcgca cgccctcttc agccacaaag gcaaaatccg gctcgcgcaa gctttcatcc
      121 caggcaatgg caatgccggt ttcacgggca aacgctcgcg aatcatcgcg ggttttgcac
      181 ggctcttcga gaaacgcgat gcggtggcga taatccgggt taacatattt ggcaaactgc
      241 tggcctttca gcggtgtcca ggcgcgattt gcgtcaagac gcaaatgcag atccggaatt
      301 gcctccagca acagattaac caccatgccg tcgcgcacgg cttcgtacaa tcccactttg
      361 accttcgcca ctttctcgcc tggcatatca gcaagtttga ggatcagatc gtcaggatcg
      421 ccattacaca gtggtgccgc acggtaattg gccgcctgcg ggagcgtatc tgccagctcc
      481 gccaatgcac agcttacgcc aaaggcaacg gaagtcatct gcggtagctc gcaatcgcct
      541 gccagccagt tatttaccca ggcaagcagc acgctttgcg cctcttccca ggtttcctgg
      601 ctgaagcccg gcagtgggga gatctccccc cacccttcgc gctcgccctc acgcaggcaa
      661 acgtacagcc cgtcgcgggt ttttaaccgc ctgtcgcgca gaaccacccc cgcgtccatg
      721 gggatctgcc agcggtatac ctgcgcgcta cgcattacgg attccgttta aatttgctga
      781 aatcaggctg acgtttctgg ttgaaagcgt tgcgaccttc ctgaccttct tccgtcatgt
      841 agaacagcat ggtggcgttg cccgccagct cctgcagccc cgcctgcccg tcacagtcgg
      901 cgttcagcgc agctttcagg cagcgcagcg ccatcgggct gttttgcagc atttcgcggc
      961 accaacggac ggtttctttt tccagatccg ccagcggtac cacggtgttg acaaggccca
     1021 tatccagcgc ctgttttgcg tcgtactgac ggcacaggaa ccagatttct cgcgcttttt
     1081 tctgcccgac gatacgagcc atgtaggaag cgccccagcc gccgtcgaag gaaccgactt
     1141 tcgggccagt ctgaccgaag atggcattat ctgctgcgat agtcaggtcg cacatcatgt
     1201 gcagaacgtg accaccgccg atggagtagc cagccaccat cgcgacaacc ggtttcggac
     1261 aggtacggat ctgacgctgg aagtccagca cgttcaagtg atgtacgccg gaatcatctt
     1321 tatagccgcc gtaatcacca cgcactttct ggtcaccacc ggagcagaac gctttatcgc
     1381 ctgcgccagt cagaatgatc acgccgatat tgtcgtcata acgcgcatct gccagtgcct
     1441 ggatcatctc ttttaccgtc agaggacgga aggcattgcg cacctgcgga cgattaatgg
     1501 tgatttttgc gataccgtcg gtggattttt cataacgaat gtcctcgaaa ccttcggagc
     1561 agtcgtgcca ttcaaccggt gcgtaaagca ttgcttcatc aggataaatc atattgtgtc
     1621 ctttagtcag aaacgcaaga tctgcgccag acttgcgatt acgccagcgg gattttcccg
     1681 atgcgcgtta tgtccggcgc gaggaatgac atggcagtcg gcagccagtt ccgccgccag
     1741 ggcgcggaat ttgctgtcac gttcaccaca taaataataa aacgcaaatg tgcgggcgct
     1801 aaggttagca cgtaaatcag gctggacggc gagagaagtc gcctccagca tggcggcaag
     1861 ggttgcgcca ttattgttgc tgcgcagcac caccagctcc cggcgttgat cgtcattgag
     1921 cgaggcaaaa acaggctgtt gataccagtc ggcaaatacc gccgttaacg gttctgtgca
     1981 aaaacgctgc gcccattggc gatcggaacg ctgacgttcc gcacgttgtt cagcattttg
     2041 cagccccgga tgcccgcctt cgacaataac cccacaaagc cccgtcagcc cctggcaagc
     2101 cgccatcatc gccacccgtc cgccaagcga gtaccccacc agccagaagt caaggatgtt
     2161 gtaactaacc aaggttttac gcagtaagtc ggtgacatca tcaaatccat cgacgctaat
     2221 cgccgccgaa ccaccatgac ctgggagatc aacatacaac cgtgagtagt cggcaaacgc
     2281 ctcgcccact tcttgccatt cgtggcaatc gccggaaaaa ccgtgcaaaa acaccagcca
     2341 gggtaaacct ggttttccgt gttttgcctg cgcgtgcagg atcataaatg gcttacctgc
     2401 gccagaagtt gctggagcgt ttgcgcacca tcggtgtcgt taaccaccat ttcaatcacc
     2461 gtggtggttg gcgtgcgcca ggcgtcggca aatgccgttt caagttcctg ccagttttgc
     2521 ggacgatgat atttcagctc gaacatcgcg gcggcgtgct caaaatggac gttttgcggc
     2581 atcagataga aacgctcacg ctcgctttgc ggcgttggca acagcgagaa aatttgcccg
     2641 ccgttgttgt tcaccacaat taataccagc ggcgcagaaa cctgacgcaa taacgccagc
     2701 gcgttgagat cgtaaagtgc ggagagatcg cccacaatcg ccagcgtcgg tttgccgctt
     2761 gcccgctgaa cgcctgcggc ggtcgaaagc agtccgtcga taccgctggc ccctcggttg
     2821 ctgtacaccg ggtaacctgc cggaagttgc gaaagcgcat caatcagacg taccaccagg
     2881 ctgttaccaa caaacaattg cccctgttca ggcagatagt cgcagatgcg atgcgccagt
     2941 tgcgcttcgc caaacgcatc acggcgggca ataaccgcct gcattgcctg ttccgccagg
     3001 cgcgggattt caacgcacca gggctggcgt ttttctgccg gatgcagctc cagccagtcg
     3061 gcaatattgg caattaagcg acgtccgcga tggtgtgccg gatcaagtcg cccttcaatg
     3121 tcatcaacaa tccagtactc ttctggttca cagcttgcct gccattgcag gagccgtttc
     3181 cccgtcaggc tgcttcccag ttgcaccaca atttgcgcct gctgcagctc gctggtcgct
     3241 ttggcattgc ctaaccaaag atcggcacac ggcagcggct gcccggtttg tgacagcaca
     3301 tcgccaatca gcggccagcc aagagtttgc gcccacaggg caactttttt gccctcttcc
     3361 gcactcatgc gcccggcaac caccacgccg cgcttttgtc gccagaagaa ccagtcgcgc
     3421 tgtttttcac tttccagacg aggcgcttca cgcagccacg gtttgtcgtc ctgccaccag
     3481 tcacccagac gctgttgcca gctaagcccg gtatcgtcca tttcgccata cagcggttca
     3541 gcaaacgggc agttgatatg gactcccccc gcatgaagcg taccgagagc gtggtcgatg
     3601 gtagaaacca gccaacgtgc ggggatatcc tgggtcgggc gcggcaatga aatactgtgc
     3661 gtggggtgag aggcgaacat tcccggctgg cgaattgcct gattcgcgcc gcagtcaatt
     3721 agctccggcg ggcgatcggc ggttaagaga atcagttttt ctccggttaa cccggcttca
     3781 atcagtgccg gatagagatt tgccaccgcc gtgccggagg tcacaatcac cgccaccggc
     3841 tgcttgctga ctttcgccag ccccagcgcc agatgcccca acccacgctc atcgaaatgg
     3901 gtgtggtgaa tgaatgcgga attctccgcc gccgctaacg ttaacggtgt agaacgcgag
     3961 cctggggcga tacagatgtg tctgacgccg tgacgcgtta atgcttccag aatgaccgcc
     4021 gcccagcgtc ggttaaatgc gcttactgac atgagtttgt ccggtatcaa tattgcggct
     4081 aagtataagg agctacaaaa atagattatt gatatgaatc ggtaatgatg cgactcatta
     4141 ctattccatt tgtaataaag tacgcagccc tgccgctttg ttgtcgattt cctgccactc
     4201 ttgctcgggg tcggaaccac ggacaatgcc cgcgccagca tataatcgca cgacattgcc
     4261 gctaattttt gctgagcgca gggaaacgca gaattcgctt tgttgtaatg agagatagcc
     4321 cgcagaaccg gcgtaccatt ctcgggtgaa cggttcgtga cgggcgataa actgtcgcgc
     4381 cagatcgcgc ggtaagccag caactgctgc cgttggctgc aactgatgta aacagatcac
     4441 atcatccgct ttgttgagtg aagtccagat acagcggcga agatgctgca ctttacgcag
     4501 acgcagtacc tgcggcggta aaacatccag cgtctgggta tcggcctgta atcgttggca
     4561 gatatcttcc accaccagca tgttctcgcg ctggttttta tcatccgcca tcagccactc
     4621 tcctaactgc tgcgcctgct tatcatcagg attatttgct actgttcccg ccagcgcttc
     4681 agtacgcagc gctttgtcac gccgccgcca taaccgttcc ggtgaagagc caagaaaagc
     4741 attttcgcca tcaaaggcca tgtaaaaatg gtagcaattc agattcagtc gacgactggc
     4801 agccatcatc gccgccgcgt tgaccggact tgcgaaatgc aggtcagttg cccgagcgag
     4861 caccactttg tcgagctcac cttcggcgat ggttttcgtt gccagttcga ttaattgcgt
     4921 ccagcccgtt ttgtccggcc agtgttgttc tcgcgtggtg gttaaatgta acccaggcaa
     4981 gggcttgata ctcaccagtg tggcgataaa ttcttttgcc tgaatcgcat cgtgctgaag
     5041 ggagctttcg ctgaatagcg tcagccgcag cgtggcttta ccgccacagc gtcgccattc
     5101 caggcggggt aaaagtaaat tgccctgcga cgggtcaaaa gcattcagcc cccaaatgcg
     5161 taagtcggcg tgttccgggt gctggcgaag aaaacgttgt gcctggtcca acgacgtaaa
     5221 acgggtaatc gcgcccagga cggcagcttc ttcatcacca ttacgttgtt gccagtagaa
     5281 ttgcgggtac gtttgctgac tggccagcca gctcaaggca tcaaaagcgt ctttgagagg
     5341 gaaaggaata tcgataaccc gaatgccggg tgtcgccgga atctcttgcg acaaatggcg
     5401 cagtagattt tccagcgccg tagtaagtga ttgcacgcgg acctctccct gttaaaagcc
     5461 tcacattata cggggtacta caaaaaaaat gcagtacccc ggtgtaggga ggttttaacg
     5521 gcgtgccagc aacagtccta gtaccagccc aacggccgcg cccacaccaa ttccttgcca
     5581 gggtttttcg tggacgtagt catcagcacg ataaactgcc tgcttcgccc gatagtaata
     5641 actgtctgaa gcctggctaa cccgtttttt cacatcatcc agcgcttttt ctgcacgcgc
     5701 tttcagctca acatatttct gatcggcggt atcgccagag gagcggagca cctcttccag
     5761 tgtttcacta agcagcgtca ggtcgtcatc gatacgtgta tcaccaaact gattagacat
     5821 tctcgttctc catttgcgta aaacctgtca ctaactatag acaagggttg ttgattacgc
     5881 ctgaattacc tcgcgtgcca tcccaatgtg tgggatacca tcttcttcat agacctccgt
     5941 cacagggata aaaccgaaac tctggtagaa gttttgcaga tgcgcctgcg ccccgaggta
     6001 aacaggctta tcaggccagt gatgcgtaca tgtttcgagt gttttactca tcagctgctg
     6061 ccccactttt tcgccacgca acgcttcact gacaatcacc cgacctataa cgaccggctc
     6121 aagatcatca tcacttttca gaatcctcgc atatgccacc agttcatcat ttttccaacc
     6181 gagaatatgg cgattatccc ccgtcaaatc atcaccatcg atatcctgat aagggcaatt
     6241 ctgctcgaca acaaacaccg cgcaacgcag ttgtaataag gcataaagct gggaaaccga
     6301 taattcagag tgatgcagat cttgccattc aatcataatg ctctcctttt agatgtatgc
     6361 cttatactag ccggaatatt ttttgaaacg tgatgaactc atggaattaa tttttttagg
     6421 tacttcggcc ggtgtgccaa cccgcacgcg caatgtcacg gcaatattgc ttaacctgca
     6481 acatccgacc cagagcggtc tttggttgtt tgactgcggt gaaggcaccc agcatcagct
     6541 actgcatacc gcctttaacc ctggaaaact ggacaagatt tttatcagtc accttcatgg
     6601 cgatcatctt tttggtttac ccggcttgct gtgcagtcgt tctatgtcag gcattatcca
     6661 acccttaacg atttatggtc cgcaaggtat ccgtgaattt gtggaaaccg cgctgcggat
     6721 tagcggctca tggaccgatt atccgctgga aattgtcgaa attggcgctg gcgaaattct
     6781 cgatgatggc ctgcgcaaag taaccgctta tccgctggaa cacccactgg aatgttatgg
     6841 ctatcgtatt gaagaacatg ataaaccggg tgcattaaat gcccaggcat taaaagctgc
     6901 tggcgtgccg cctggcccac tgtttcagga attaaaagcg ggcaaaacaa tcacgctgga
     6961 agatggaagg cagattaacg gcgcagatta cttagctgct ccagtgccag gtaaagcgct
     7021 cgctattttc ggcgataccg gcccctgcga tgccgcactt gacctggcta aaggtgtcga
     7081 tgtcatggtg cacgaagcga cgctggatat aaccatggaa gccaaagcca atagtcgcgg
     7141 ccatagctct acacgccag
//
LOCUS       DAMZET010000195         7109 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.195, whole genome shotgun sequence.
ACCESSION   DAMZET010000195 DAMZET010000000
VERSION     DAMZET010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.195"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(177..389)
                     /gene="rpmE"
                     /locus_tag="POB34_004157"
     CDS             complement(177..389)
                     /gene="rpmE"
                     /locus_tag="POB34_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDI5921010.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            592..2790
                     /gene="priA"
                     /locus_tag="POB34_004158"
     CDS             592..2790
                     /gene="priA"
                     /locus_tag="POB34_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDI5921011.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQVGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2946..3971
                     /gene="cytR"
                     /locus_tag="POB34_004159"
     CDS             2946..3971
                     /gene="cytR"
                     /locus_tag="POB34_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HDI5921012.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4063..5022
                     /gene="ftsN"
                     /locus_tag="POB34_004160"
     CDS             4063..5022
                     /gene="ftsN"
                     /locus_tag="POB34_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HDI5921013.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVTRAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5115..5645
                     /gene="hslV"
                     /locus_tag="POB34_004161"
     CDS             5115..5645
                     /gene="hslV"
                     /locus_tag="POB34_004161"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDI5921014.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5655..6986
                     /gene="hslU"
                     /locus_tag="POB34_004162"
     CDS             5655..6986
                     /gene="hslU"
                     /locus_tag="POB34_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HDI5921015.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
BASE COUNT         1762 a         1882 c         1934 g         1531 t
ORIGIN      
        1 gccggttttc cgctcatcgt aaaatccttt tgaaataaat aagaaccaga agcctagcgc
       61 cagtcaacaa caggaaatga taatgagtta acaccccgga atttttcaga atttataaaa
      121 ggcaaattac aggcacaaaa aaagcgccgt gcggcgcttt tttcggaaat ccggtcttat
      181 ttgctgcccg ggatgttgaa acgcttgttg aagcggtcaa cacggccacc ggtagcaaca
      241 tcacgctgtt tgccagtgaa gaacgggtgg cacttgctgc acacgtcgag gttcaggtca
      301 tgaccaacgg tggagcggat tttcattacg ttaccgcaag agcagctagc agtaatttct
      361 tcgtatttcg ggtgaatatc ttttttcatg ggaaaacctc ggtttaaggc cgcgtcgctc
      421 ttccagccct aacgccagac accacgcgat gttaaaagta tagcttcaat acgatcattt
      481 cgtacgaagg cgcgaaatca tacagaaatt aaccagcgta tgcaaactga tccgcactct
      541 tctacggcaa tgtgtatact aacccaccga atttcaagtc aggatgatgc tatgcccgtt
      601 gcccacgttg ccttgcccgt tccgcttcct cgtacctttg actatctgct gccagaaggc
      661 atgacggtta aagctgggtg tcgcgtgcgc gtgccgtttg gcaaacagca ggagcgcatc
      721 gggattgtgg tatcagttag cgatgccagc gaactgccgc tcaatgagct aaaagcggta
      781 gtcgaagtgc tggatagtga gccggtgttt actcactccg tctggcgatt gctgctatgg
      841 gcggcagatt actatcatca tccgattggc gatgtgctgt ttcatgcctt gccgatttta
      901 ctacgccagg ggcggcctgc ggcgaacgcg ccgatgtggt actggtttgc cactgaacaa
      961 ggtcaggcgg tggatctgaa cagcctgaaa cgctcaccca agcaacaaca ggcgctggca
     1021 gcgttacggc aaggcaaaat ctggcgcgac caggttgcca cgctcgaatt taatgatgcc
     1081 gcgttgcagg cgctgcgcaa aaaaggtctg tgtgatttag caagtgaaac accagagttt
     1141 agcgactggc gaacgaacta tgccgtttct ggtgagcggt tgcgattgaa taccgaacag
     1201 gccaccgccg ttggcgcaat tcatagtgcg gcggatacct tctctgcctg gctgctggcg
     1261 ggcgttaccg gttccggtaa aacggaggtt tatctcagtg tactggaaaa cgtgctcgct
     1321 cagggcaaac aggcgctggt gatggtgccg gaaatcggcc tgacaccgca aactatcgcc
     1381 cgttttcgtg aacgttttaa tgcccccgta gaagttctgc attccggctt gaacgacagc
     1441 gagcgtcttt cggcgtggtt gaaagcgaaa aatggtgagg cggcgattgt gatcggcacc
     1501 cgctccgcgc tgtttacgcc gtttaaaaat ctcggtgtga ttgtcattga tgaagagcac
     1561 gacagttcct acaagcagca ggaaggctgg cgctatcatg cccgcgacct ggcggtttat
     1621 cgtgcgcaca gcgagcaaat cccgattatt cttggctccg caacacccgc actggaaacg
     1681 ttatgcaacg tccagcagaa aaaataccgc ctgctgcgcc tgacgcgtcg ggcggggaat
     1741 gcgcgtccgg caattcaaca cgtgctggat ttaaaaggtc agaaggtgca ggtggggctg
     1801 gctccggcgt taatcacccg tatgcgccag catttacagg ccgacaacca ggttatcctc
     1861 tttcttaacc gccgtggctt tgcgcctgcc ttgctgtgcc acgactgcgg ctggattgcc
     1921 gaatgtccac gttgcgatca ctactacacg ctgcatcagg cgcaacagca tttgcgctgt
     1981 caccactgtg acagccaacg cccggtgccg cgccagtgcc cttcctgcgg ttccacgcac
     2041 ctagtacccg tggggctggg caccgaacag cttgaacaga cgcttgcgcc gatgttcccc
     2101 ggcgtgccca tttcacgtat cgaccgcgat accaccagcc gcaaaggggc gctggaacag
     2161 caactggcgg aagtgcatcg cggcggcgcg aggatcttga ttggcacgca aatgctggcg
     2221 aaaggtcacc actttccgga tgtgacgctg gtggcgttac tggatgtgga cggcgcgctg
     2281 ttttctgccg attttcgctc ggcagaacgt ttcgcccagc tttacaccca ggtcgccggt
     2341 cgtgccgggc gtgcgggtaa acagggcgaa gtggtgctgc aaacgcacca tccggaacat
     2401 cctctgttgc aaacgttgct ctataaaggc tacgacgcct ttgccgaaca ggcgctggct
     2461 gagcgacgca tgatgcagct gccgccgtgg accagccatg tgattgtgcg tgcggaagat
     2521 cataacaatc agcatgcgcc gctgttcctg caacaactgc gtaatctgat cctttccagc
     2581 ccgctggcag acgacaaact gtgggttctc ggcccggttc cggctctggc acctaagcgt
     2641 ggtggccgct ggcgctggca gatcttgttg cagcaccctt cccgcgtgcg cttgcaacac
     2701 atcattagtg gtacgctggc gcttattaat acaataccgg attcccgcaa ggtgaaatgg
     2761 gtgttggatg tcgatcccat cgaaggctaa tgcgcaaacg ttgcgaggcg gatcgaaaaa
     2821 ttcaatattc atcacacttt tcatgaaaat tctgtaaccg ttttcacgcg ctatctgcta
     2881 aaaatgttgc cgatgtgaag taaacatgga tgtagtacgc ctgacgtgcc aggcgaggag
     2941 tgagtgtgaa agcgaagaag caggaaactg ccgcgaccat gaaagacgtt gccctcaagg
     3001 caaaagtctc tacagcgact gtctcccgag cattaatgaa tcccgataaa gtctcccagg
     3061 ccacccgtaa tcgggttgaa aaagcggccc gggaagtggg ttatttaccg caacctatgg
     3121 ggcgcaacgt caagcgtaat gaatcccgca ccattctggt gattgtcccg gatatctgcg
     3181 atcccttctt tagcgaaatt attcgcggta tcgaagttac ggcggcaaat cacggatatc
     3241 tggtgctgat tggcgactgt gcgcatcaaa atcagcagga aaaaaccttt atcgatttga
     3301 tcatcaccaa gcaaattgat ggcatgttgc tgctgggctc aaggctgccg tttgatgcca
     3361 gcattgagga acagcgtaat ctgccgccga tggtgatggc gaacgaattt gcaccggaac
     3421 tggagctgcc taccgttcat atcgacaatc tgaccgccgc atttgatgca gtaaattatt
     3481 tatatgagca agggcataaa cggattggct gtatagccgg tcccgaagag atgccactgt
     3541 gtcactaccg cctgcaaggc tatgttcagg cgctgcgtcg ctgcggcatt atggttgatc
     3601 cgcaatacat cgcccgtggc gacttcacct tcgaagccgg aagcaaagcg atgcagcagc
     3661 tgcttgatct tccacaaccg cctactgctg tcttctgcca tagcgatgtg atggcgctcg
     3721 gcgcactttc tcaggcaaaa cgccaggggc tgaaagtccc ggaagacctt tccataatcg
     3781 gttttgataa catcgacctg acgcaatttt gtgatccgcc gctgacaacc atcgcgcagc
     3841 cgcgttacga aatcggtcgg gaagctatgc tgttattgct tgatcaaatg caggggcaac
     3901 acgttggcag tggctctcgt ttaatggact gcgaacttat catccgggga tcaacacgcg
     3961 cgttacctta aagtaaacct ttaagactgc cgtgactggt caaaggcccg ccgcttaagt
     4021 aacatggcgg gctgacgaac gaataaatac agcgaaacga tagtggcaca acgagattat
     4081 gtacgccgca gccaaccggc accttcgcgg cgaaaaaaga gcacctcacg gaaaaagcaa
     4141 cgaaatctgc ctgcggtttc tcccgctatg gtcgctattg ctgccgccgt tcttgtgacc
     4201 tttatcggtg gtctgtactt cattacgcat cacaagaaag aagagtccga gacgctgcaa
     4261 agccagaaag tgaccggaaa cggactacca ccaaaaccag aagaacgctg gcgctacatc
     4321 aaagagctgg aaagtcgcca gccgggtgtg cgtgcgccca cagaaccttc tgctggtggt
     4381 gaagtgaaaa cgccggagca actgacacca gaacaacgtc agcttcttga acaaatgcag
     4441 gctgatatgc gccagcagcc aacgcagctg gttgaggttc cgtggaatga acagacgccg
     4501 gaacagcgtc agcaaacgct acagcgccaa cgtcaggcgc agcaattagc ggaacagcaa
     4561 cgcctggctc agcagtcgcg tacgactgaa caaagctggc agcagcagac gcgtacgtct
     4621 caagccgcgc cggtacaggc gcaaccgcgc cagtctaaac cggcttctac tcagcagccg
     4681 taccaggatc tgctgcaaac ccctgcgcac acaaccgcgc agtcgaagcc gcagcaagct
     4741 gcgccggtta ctcgtgccgc tgacgcgcca aaaccgacgg cggagaaaaa agacgaacgc
     4801 cgctggatgg tgcagtgcgg ttcgttcaga ggcgcggaac aggcagagac ggtacgtgct
     4861 cagctggcgt tcgaaggatt tgactcgaaa atcaccacca acaatggctg gaatcgtgtg
     4921 gtcattggcc cggtgaaagg caaagagaac gcagacagca ccctcaatcg gttgaagatg
     4981 gcgggtcata caaactgcat tcggctcgcc gccgggggtt gaaaccctca aaatcccccc
     5041 catctataat tgcattatgc cccgtacttt tgtacggggt ttgtactctg tattcgtaac
     5101 caaggggtca gctcgtgaca actatagtaa gcgtacgccg taacggccat gtggtcatcg
     5161 ctggtgatgg tcaggccacg ttgggcaata ccgtaatgaa aggcaacgtg aaaaaggtcc
     5221 gccgtctgta caacgacaaa gtcatcgcgg gctttgcggg cggtactgcg gatgctttta
     5281 cgctgttcga actgtttgaa cgtaaactgg aaatgcatca gggccatctg gttaaagccg
     5341 ccgttgagct ggcaaaagac tggcgtaccg atcgcatgct gcgcaaactt gaagcactgc
     5401 tggcagtcgc ggatgaaact gcatcgctta tcatcaccgg taacggtgac gtggtgcagc
     5461 cagaaaacga tcttattgct atcggctccg gcggccctta cgcccaggct gcggcgcgcg
     5521 cgctgttaga aaacactgaa cttagcgccc gtgaaattgc tgaaaaggcg ttggatattg
     5581 caggcgacat ttgcatctat accaaccatt tccacaccat cgaagaatta agctacaaag
     5641 cgtaaggatc tcccatgtct gaaatgaccc cacgcgaaat cgtcagcgaa ctggataagc
     5701 acatcatcgg ccaggacaac gccaagcgtt ctgtggcgat tgctctgcgt aaccgctggc
     5761 gtcgcatgca gctcaacgaa gagctgcgcc atgaagtgac cccgaaaaat atcctgatga
     5821 tcggcccgac cggtgtcggt aaaactgaaa tcgcccgtcg tctggctaag ctggcgaatg
     5881 cgccgttcat caaagttgaa gcgaccaaat tcaccgaagt gggctacgtc ggtaaggaag
     5941 tggattctat tattcgcgat ctgaccgatg ccgccgtgaa aatggtacgc gtccaggcta
     6001 tcgagaaaaa ccgttatcgc gctgaagaac tggcagaaga acgtattctc gacgtgctga
     6061 tcccacctgc taaaaacaac tggggacaga ccgaacagca gcaggaaccg tccgctgctc
     6121 gtcaggcatt ccgcaaaaaa ctgcgtgaag gccagcttga tgacaaagaa atcgagatcg
     6181 atcttgccgc agcaccgatg ggcgttgaaa ttatggctcc tccaggcatg gaagagatga
     6241 ccagccagct gcagtccatg ttccagaacc tgggcggcca gaagcaaaaa gcgcgtaagc
     6301 tgaaaatcaa agacgccatg aagctgctga ttgaagaaga agcggcgaaa ctggtgaacc
     6361 cggaagagct gaagcaagac gctatcgacg ctgttgagca gcacgggatc gtgtttatcg
     6421 acgaaatcga caaaatctgt aagcgcggcg agtcttccgg tccggatgtt tctcgtgaag
     6481 gcgttcagcg tgacctgctg ccgctggtag aaggttgcac cgtttccacc aaacacggga
     6541 tggtcaaaac tgaccacatt ctgtttatcg cttctggcgc gttccagatt gcgaaaccgt
     6601 ctgacctaat cccggaactg caaggtcgtc tgccaatccg cgttgaactg caggcactga
     6661 ccaccagcga cttcgagcgt attctgaccg agccgaatgc ctctatcacc gtgcagtaca
     6721 aagcactgat ggcgactgaa ggcgtaaata tcgagtttac cgactccggt attaaacgca
     6781 tcgcggaagc ggcatggcag gtgaacgaat ctaccgaaaa catcggtgct cgtcgtttac
     6841 acactgttct ggagcgttta atggaagaga tttcctacga cgccagcgat ttaagcggtc
     6901 aaaatatcac tattgacgca gattatgtga gcaaacatct ggatgcgttg gtggcagatg
     6961 aagatctgag ccgttttatc ctataatcgc gttcaatcat tttcatcatt gtttgatggg
     7021 gctgaaaggc cccattttta ttggcgcgta ttatgactga acaacaaatt agccgaactc
     7081 aggcgtggct ggaaagttta cgacctaaa
//
LOCUS       DAMZET010000196         7076 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.196, whole genome shotgun sequence.
ACCESSION   DAMZET010000196 DAMZET010000000
VERSION     DAMZET010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.196"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(151..1761)
                     /gene="yeaV"
                     /locus_tag="POB34_004163"
     CDS             complement(151..1761)
                     /gene="yeaV"
                     /locus_tag="POB34_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HDI5921016.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTAVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            complement(2012..3097)
                     /gene="dmlA"
                     /locus_tag="POB34_004164"
     CDS             complement(2012..3097)
                     /gene="dmlA"
                     /locus_tag="POB34_004164"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HDI5921017.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            3200..4123
                     /gene="dmlR"
                     /locus_tag="POB34_004165"
     CDS             3200..4123
                     /gene="dmlR"
                     /locus_tag="POB34_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HDI5921018.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPPGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            4250..4888
                     /gene="leuE"
                     /locus_tag="POB34_004166"
     CDS             4250..4888
                     /gene="leuE"
                     /locus_tag="POB34_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HDI5921019.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NSEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            5061..5420
                     /gene="yeaR"
                     /locus_tag="POB34_004167"
     CDS             5061..5420
                     /gene="yeaR"
                     /locus_tag="POB34_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HDI5921020.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYFGYPDEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            5424..5606
                     /locus_tag="POB34_004168"
     CDS             5424..5606
                     /locus_tag="POB34_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HDI5921021.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            5754..6002
                     /locus_tag="POB34_004169"
     CDS             5754..6002
                     /locus_tag="POB34_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDI5921022.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(6061..6135)
                     /gene="yoaJ"
                     /locus_tag="POB34_004170"
     CDS             complement(6061..6135)
                     /gene="yoaJ"
                     /locus_tag="POB34_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HDI5921023.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6138..6236)
                     /locus_tag="POB34_004171"
     CDS             complement(6138..6236)
                     /locus_tag="POB34_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HDI5921024.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            complement(6269..>7076)
                     /gene="dgcP"
                     /locus_tag="POB34_004172"
     CDS             complement(6269..>7076)
                     /gene="dgcP"
                     /locus_tag="POB34_004172"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HDI5921025.1"
                     /translation="YSLCKRAIDENCFFSDEVPDRWGDCIAARNLGITTFLSTPIHLP
                     DGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALVEQLREANAALIAQSY
                     TDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKLINDRFGHNSGDLFLI
                     QVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERIQQQIRGEYHLGDVDL
                     YYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFVAHPALHS"
BASE COUNT         1852 a         1661 c         1772 g         1791 t
ORIGIN      
        1 tctttttcgt gttcaaacgc gttctgatcg gtataaaaac gggcaggaat ggtccacgcc
       61 tcttgcgggt tagcgcaaaa attttcgggt agtacaaagt cagggctcag attgctcata
      121 gggatacctt tatcgttagg atgatggcgc tcagttgtcg cctttgaata cggttcccgc
      181 agggagcact ggcgtttttt ccagggcttc aaccggtgtc tgcgggagat aatgttcaac
      241 ttgatgagcc ggaatgtcgg cgtaatcctg tttcagccac cgaataaatc cgccgacttt
      301 caccagtaaa atgacgagga agggaagggc tgtgagcacg acggcggttt tcatcgtttc
      361 cagcgaagca ccggtaaaca agatggaaag cgggatcaga gtgatcacca cgcaccagaa
      421 aagacgcagc ccacggtcag gatcgtcacc ttcctggaga ttacgcgtac tggtcgcagc
      481 catggtgtag gccaccgcat ccatatgcga ggcaaggaaa ataatcatca cgcccaggta
      541 tgcggcgagg aacaatttac cggctggcaa cgacatcaga acttgctgta cagctgtttc
      601 gccgcccagt gtttccagca cctgtgggac gttgattacg ccattgataa actgatgaat
      661 ggcatagctt tccattacgc cgaagaagaa ccagcaaccg acggtgctgc cgaggatcag
      721 tccccagata acttctttaa tcttacgacc gcgggaaacg cgggtgacaa acattgctac
      781 gccaggggtg tatgagatcc accacagcca gtagaaaacg gtccagttgc gggtaaatga
      841 accatcgcca agcggatcgg tgaataagct catttgcagg aagttttgcg tggtcagacc
      901 gatggcattg atgatgctgt tggtaataaa ttcggttggg ccgacaatca gcaccagtaa
      961 tggcagcagg aacgcgcccc agccaaccat tttgctcaga cgttgcaaac cgttgttgat
     1021 accaatccac gagcttaggc aaaaaatgcc gccggaaagc aggatcacaa atgcctgcac
     1081 ggtgaagtta tcgggtaaac cggtcagcgc ggaaagtcca cgagtaaagg ttgctgcggt
     1141 gacaacaagg gaaatagtca gtgcgccgac agtggcgatc aggaacatca aatcaaccag
     1201 ttttccccat gggccttgcg ggcgaacgcc ggtaatcgcg gcaataatgc cggaaagact
     1261 cagacctttg tttttacgca catgaaagtg ataagccatg attaatgagg ccagcgtata
     1321 agttgcccag gcgctgatgc cccagtggaa gaaggagtaa gggacgctaa attcgagtgc
     1381 ctgttgtgaa cgcggtgcga tatttaatcc aggcgtttga taataatagg cccattcagc
     1441 aaccccccaa taaagcgtag aagaacctaa accggcacaa ataaacataa acagccaaga
     1501 gagcgtgctg tattccggtt ttccttcgcc aagacgaata ttgccgtatt tactggtggc
     1561 taaataaatc accagtccca ttgccagcaa aaccaatacc tgtacggcgg aacctaacgt
     1621 acgcgtaaca aaagtgtata ttgcgttggc cgcgtcggcc gattgttgtg ggaaaaggct
     1681 taatgcagct gcgatgaaaa gaatggccac caggctgatg cttatcagcg gcacatcttt
     1741 tttcttaaca ttgctcatca tgagtcatct cctgggcata aaagcccgtc tctggctttt
     1801 gccagttgta attatttgca gggtaatgag ttgtatgttg tgtttaacaa attgattatt
     1861 tgcatgttac ataaattaaa caattaagaa tacggaaagc cataaaacac gaaacggaaa
     1921 caataaaagg tgaaatgacg caggttattt actgcaaaga acgaataaaa aaaccgcgtc
     1981 agtgacgcgg ttcaaataaa ctggtttgac cttaacgcaa aataattttg caaatcgcgt
     2041 cggcaacctg tggcgtggtg gcattgcctt tcatatcagg tgttttcggc ccgtgagcaa
     2101 tcacttcttc aattgctgcc agaataccgt tatgcgcttg ctggaaacgc tcatcgccat
     2161 tgccgagaaa atcgagcatc attgcaccgg cccaaatcgt ggcgataggg ttagcaatgt
     2221 ttttcccgta gatatccggc gcggaaccgt ggactggctc gaagagcgac gggaaagtgc
     2281 gttccggatt caggttggcg gatggggcaa tgccaatggt gccggtgcag gccgggccaa
     2341 gatcggaaag gatatcgcca aataaattgg acgccaccac cacatcgaag cgttccggct
     2401 gcatcacaaa acgcgcgcag agaatatcaa tatgctgctt gtcccagcgg atctccgggt
     2461 aattctcggc cattgcttcc actcgctcat cccagtacgg catgctgatg gctaaaccgt
     2521 ttgatttagt ggcagaagtt agcgtcttac gtgggcggct ttgcgcaagt tcgaaggcat
     2581 aacgcaaaat gcgatcgaca ccacgacgcg taaataccga ttcctgaatg acgacttcat
     2641 gctctgtacc ttcattcact ctaccgccga gcgaggaata ttcgccttcg gtgttttccc
     2701 tgaccacgta aaaatcgatg tcgccaggct gttttcccgc cagcgggcag ggaacgccag
     2761 gaaagagacg aaccgggcgc aggttgacgt actggtcgaa ttcacgacga aatttcagca
     2821 gcgaacccca caacgaaata tggtcaggaa cggtgtccgg ccagccgacg gcaccaaaat
     2881 agatggcgtc gaaacggcta agttgctcat gccagtcgtc cggcatcatt ttaccgtgat
     2941 ggctgtaata ctcgcagctc gcccactcca tttgctcaaa actcaaggcg aagccccagc
     3001 gctcagcggc agcctgtaac acgcgaatcc cttcaggaag gacttctttg ccaatcccgt
     3061 ctcccgggat cgcagcaata cgcatcgttt tcatcattgg gttctcactt cctgaccatc
     3121 aaaattgaca acatcaccat cctataattg actgaaaagg aattaatccc cgtaactggt
     3181 gaaacataaa acacgaatca tgaataattt accgctgctg aatgatttgc gcgtctttat
     3241 gctggtggct cgtcgggctg gttttgccgc cgtggcggaa gaactgggcg tttcaccggc
     3301 gttcgtcagc aagcgcatcg ccttgctgga gcaaacgcta aacgtggtgt tgttacaccg
     3361 caccacccgc cgcgtcacca ttaccgaaga aggagagcgg atttatgaat gggcgcagcg
     3421 gattttgcag gatgtcggtc agatgatgga cgaactttct gacgtgcgtc aggtgccgcc
     3481 ggggatgctg cgtattatca gcagctttgg ttttggacga caggtggtgg ctccggcgtt
     3541 atcagcgctg gctaaggcgt atccgcaact ggaattacgt tttgatgtgg aagaccggtt
     3601 ggtcgatttg gtcaatgaag gtgttgatct cgatattcgc attggcgatg acatcgcccc
     3661 taatttaatc gcccgcaaac tggcgactaa ttaccgcatt ctctgcgcgt cgccggaatt
     3721 tattgcgcaa catggcgcgc cgaaacattt aaccgatctt tccgcattgc cgtgcctggt
     3781 cattaaagag cgcgatcatc cttttggtgt ctggcaatta cgcaataaag aaggccacca
     3841 tgcgattaaa gtcaccggcc cactgtcgtc taatcatgga gaaattgtcc atcagtggtg
     3901 tctcgatggg caggggattg ctctgcgctc ctggtgggat gttagcgaaa acattgccag
     3961 tggtcattta gtgcaagtgc taccggaata ttaccagcca gcgaacgtct gggccgttta
     4021 tgtttcaagg ctagcgacgt cagcgaaagt gcggataacg gtagaatttt tacgccagta
     4081 ttttgccgag cactacccga atttttcact ggagcatgcc tgattaatga ttcaattatc
     4141 gggttgatat caggttaaaa cctgattttc tcctttctaa gccgctacag attggttagc
     4201 atattcacct ttaatcgcgc atgatcgaaa gataattaaa gaggttaatg tgttcgctga
     4261 atacggggtt ctgaattact ggacctatct ggttggggcc atttttattg tgttggtgcc
     4321 agggccaaat actctgtttg tactcaaaaa tagcgtcagt agcggtatga aaggcggtta
     4381 tcttgcggcc tgcggtgtat ttattggcga tgcggtattg atgtttctgg catgggctgg
     4441 agtggcgaca ttaattaaga ccaccccgat attattcaac attgtacgtt atcttggtgc
     4501 gttttatttg ctctatctgg ggagtaaaat tctttacgcg accctgaagg gtaaaaatag
     4561 cgaggccaaa tccgatgagc cccaatacgg tgctattttt aaacgcgcgt taattttgag
     4621 cctgactaat ccgaaagcca ttttgttcta tgtgtcgttt ttcgtacagt ttatcgatgt
     4681 taatgcccca catacgggaa tttcattctt tattctggcg acgacgctgg aactggtgag
     4741 tttctgctat ttgagcttcc tgattatttc tggtgctttt gtcacgcagt acatacgtac
     4801 caaaaagaaa ctggctaaag tgggaaactc actgattggt ttgatgttcg tgggtttcgc
     4861 tgcccgactg gcgacgctgc aatcctgata cattcagccc gcgttgtcgc gggcttccca
     4921 cttgtaatcc tccctgattt ttctctgata tggtgctaaa aagtaaccaa taaatggtat
     4981 ttaaaatgca aattatcagg cgtaccctga aacggctgga ataaaccgtt ttcagcgcat
     5041 tcaccgaagg agggaaaagg atgctgcaaa tcccacagaa ttatattcat acgcgctcaa
     5101 cgcctttctg gaataaacaa actgcgcctg ccggaatatt cgaacgtcat cttgataaag
     5161 gaacgcgccc gggggtttac ccacgccttt ccgttatgca tggggcggtc aaatatttcg
     5221 gctaccctga tgaacacagt gcagagcctg atcaggtgat ccttatcgaa gcggggcagt
     5281 ttgcggtgtt ccctccagaa aagtggcaca acattgaagc catgactgac gatacttatt
     5341 tcaacattga cttcttcgtg gctcctgaag tcctgatgga aggtgcgcaa caacggaaag
     5401 tcattcataa cgggaaatga atcatgggca aagcaacgta taccgtgacc gtcaccaata
     5461 acagcaatgg cgtttctgtc gattatgaaa cagagacgcc gatgactttg ctggtgccag
     5521 aagtggcggc tgaagtgata aaagatctgg tgaataccgt acgttcttat gacacggaaa
     5581 acgaacatga tgtttgtggt tggtaattat cactgatcgt tgatggcgca atttctgcgc
     5641 catcgcaaaa aaaacctccg tgatgctcgt cacaaatctt gccctcccga tgttgtgcta
     5701 tgctttttat cagcgactaa cccgctaatg caaaacagat aagtgaggaa acaatgggca
     5761 ttctgtcatg gattattttt gggcttattg ccggtattct ggcgaagtgg atcatgccag
     5821 gtaaagatgg cggtggattc tttatgacta tcctgctggg gatagtcggt gccgtagtcg
     5881 gcggatggat cagcacgctg tttggctttg gtaaagtcga tggcttcaat tttggcagct
     5941 tcgtggttgc cgttattggt gcgattgtcg tgctatttat ctacaggaag attaaaagtt
     6001 aacgcttaaa ttgcacaaag gctgcacaca ggcagccttt gctatttttt agaggtgacg
     6061 ttaccaccat cccagctcag tgccgagtac gacaataatc gccacaccca tcataataat
     6121 cgttgttttt ttcatcttta tgctcccggg gcagcatagc cgccaataaa aaaccatgct
     6181 aaaaatacga ccgctgtaat gaagattaca accggaaaaa taataccgat tcgcatgtta
     6241 tcaccaatca aaaggatgtg aatacgcctc aggaatgtag cgctggatgc gcgacaaaag
     6301 gtgttttctg tttgtgtttt ttctcctgat acatcgcaat atcggcagca tgcagggcac
     6361 tgtctgcatc ggttgtttca ggatcgactt ctactatgcc aagactggca ccgggataat
     6421 acaaatcaac atcacctaag tgatattctc cacgtatttg ctgctgaatg cgttctcgca
     6481 gcgacgaaat atccgcattc tcgttgttta gtgaaacaac taaaaactca tcaccgccga
     6541 gacgaccaat aacttcgcca ttttgctgga gcgtattaag gcgctcgcca acctgaatga
     6601 gaaacagatc gccactatta tgaccaaaac gatcattgat taatttgaag ttatccagat
     6661 cgataaacgc gatcattatc ttatggttaa gatgccgggc gagggaaaac agtgtcgtca
     6721 gattttcaaa aatcgcccgc cgattcggta gcccggttaa cgagtcggta tacgattgcg
     6781 caatcagcgc agcattggct tcgcgcagct gttcaaccag tgcctctttt tgaatatatt
     6841 gtgcaatcag tccggcgaat aactgtaaaa cctgttccgc gcgttcactc cactggcgct
     6901 tctcactgct ggcggcgcaa agcgtgccat agaatgatcc atccggtaag tgaattggcg
     6961 tgctcagaaa tgtggtgatg ccaagattgc gtgccgcaat acagtcaccc caacggtcgg
     7021 ggacttcatc gctgaaaaag cagttttcat caatggcgcg tttgcataac gagtaa
//
LOCUS       DAMZET010000197         7007 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.197, whole genome shotgun sequence.
ACCESSION   DAMZET010000197 DAMZET010000000
VERSION     DAMZET010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.197"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(51..758)
                     /locus_tag="POB34_004173"
     CDS             complement(51..758)
                     /locus_tag="POB34_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDI5921026.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            complement(773..1450)
                     /gene="ydjY"
                     /locus_tag="POB34_004174"
     CDS             complement(773..1450)
                     /gene="ydjY"
                     /locus_tag="POB34_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HDI5921027.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(1455..2165)
                     /locus_tag="POB34_004175"
     CDS             complement(1455..2165)
                     /locus_tag="POB34_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDI5921028.1"
                     /translation="MNAERKFLLACLIFALVIYAIHTFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASVLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(2332..3138)
                     /gene="xthA"
                     /locus_tag="POB34_004176"
     CDS             complement(2332..3138)
                     /gene="xthA"
                     /locus_tag="POB34_004176"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDI5921029.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            3584..4804
                     /gene="astC"
                     /locus_tag="POB34_004177"
     CDS             3584..4804
                     /gene="astC"
                     /locus_tag="POB34_004177"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HDI5921030.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            4801..5835
                     /gene="astA"
                     /locus_tag="POB34_004178"
     CDS             4801..5835
                     /gene="astA"
                     /locus_tag="POB34_004178"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HDI5921031.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            5832..>7007
                     /gene="astD"
                     /locus_tag="POB34_004179"
     CDS             5832..>7007
                     /gene="astD"
                     /locus_tag="POB34_004179"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HDI5921032.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     "
BASE COUNT         1680 a         1748 c         1922 g         1657 t
ORIGIN      
        1 cgcctgcgga cgtaaaatga aaggaatttt gctaagcggc ggtaaaccca tcaggcattc
       61 ctcttctggc gctcaagcca tattttcttc gccataaaaa tcaccacggt cagggcaaac
      121 agaataaaca gtccggtgac aaaccagaaa gtaccgcctg ttaacatgct gcccgcccag
      181 gaataaacaa tagtcgccgg taattgacca agcccggtgg cgataaaaaa cgagcgaaaa
      241 cgtattgaag tcagaccggc agcatagctg attggatcga aagggacaaa aggcaataac
      301 cgacagacca ggatggtgtg tttgccgtag cgagtgaaaa agccgtccat actgtcaagc
      361 acggttttgc cggttaattt ttccaccact tcgcggccca tcactctggc gataaaaaag
      421 cacagcgccg cgccggccat cgaactggtc cacgacagca agccgcccca gaacgcgcca
      481 aacagcgacg cattggcaaa ggtgatcaaa aacgcaggta gcggtgcagc aatggcctgt
      541 aaaatcatca acaagaacga gacaactgcc gccagtgcgc cgtaagactg aataaagcgt
      601 tctataccct gttggtccac ggcggcaaac gccgcaacgc tgcgattgat aaactcatgg
      661 acgccgggaa gcagcgccca tgcgagcagc atagcgaaca acaggatgat gagggttata
      721 cggtagtacc agattttacg cgattgtatc ttcatcatcc tttatcctgg ctttattcgg
      781 tgattttaaa ggtaaccgtt gccagcgtgt tatccgccgg gagaactgag gcattgcctt
      841 tgaatttcac ttcgccgcgt ttttccaccg caccataagt gtatgttgca ttgctgacga
      901 tgccgaccgg gcagctatcc agacacacca ggcaacccgt tttcttctct tctgctgccg
      961 ttaaattacc gccaaagcgc atgtccagtt tcttgccatt actgtcaaca atcacttcat
     1021 cgaaggaata cgcttttgcc gccccttgcc agttgaccga aatatccagt ttgctgcctg
     1081 tgacatgagt cgtttcttta ttatccatcg tcatgttttc gcccggcgta ccacctgcct
     1141 ctttcagggc ttcataaaat gctttcgggg tcgcataagc cataaacagc gatttatgtc
     1201 cgttggagcc atctttaaac acaataccgt gacgagtgtc gtcggtgagg aaacgaccat
     1261 taatttgcac cagcatggtt accgtatggt tattggcatc gacgcgcagc ggttggctgt
     1321 tcgaaagtcc gtcgtattcc acttttgccg ccgcattctc tttttgatcg cagccgctaa
     1381 gcccggcaac agccagtaag cagagtgcag ccagtccttt tttccatgac actgaataat
     1441 gttgcgacat cgttctatcc ttcatttttt gggtgagtaa gtgggctgcg gcgcgaagca
     1501 gacagatcca catgtttgtg tcgggcgtag agttttgcga gctggacgag aataaacagc
     1561 gccaggcccg ccagacagag ttgtaaaata aagcgcagcg taatgccttc attggcgaga
     1621 acgcttgcca tcacggtata aataacaata ccgggcaggg tcgtgagtgc cgaaataagg
     1681 gtataaggcc agaaggcgat tgtggttaat ccgtaagcgt aattttgaat attgtaagga
     1741 aacaacggga ttaagcgggt cagaataaga aaatcaatac cgttacgcgc aatgcctttt
     1801 tcaatggcct gaaaggtatg gctatgacca acgtatttca gcagtaaatc acgtcccagc
     1861 cagcgcgcca gcaggaatga gcacgaggag gcgagcgtgg cggcaattaa tgagagtagt
     1921 gtccctaaga gcgggccaaa aactattcct ccagcgatca ccagtatgct tcctggtaac
     1981 agaaagagag tggcaataat gaataacaga atatagaggc tataaccgaa caatccgctc
     2041 tggcggatga gtgtctgtaa gtggggtaaa tcggtgagca gatcgaataa accgaaagtg
     2101 tggatagcgt aaatgaccag cgcaaaaata agacaggcca aaagaaattt acgctcagcg
     2161 ttcatcgtgg agtttcctat atcaaacttg ataatagaga agtggtcaga caactaaaaa
     2221 cgcagtgacg gtataacaaa ggacggcagg caacaaatca ttactgacag tttgttgatt
     2281 tttctcaagg ttaattctcc tgacccagtt tgagccagga gagctgataa attagcggcg
     2341 gaaggtcgcc cagacggggg cgtgatcgga cggtttttcc atgctgcgaa tttcatagtc
     2401 gatgccggtt tctacacaac attctgccag cggttggctg gcgagcagca ggtcgatgcg
     2461 cagaccacgg ttatcgtcaa aaccttttga gcggtaatca aaccatgaga aacgatctgc
     2521 tgtttgcgga ttagcatggc ggaaagtatc gaccaacccc cagctcatca gcctgtccat
     2581 ccattcgcgc tcttccggca ggaaagagca tttaccggta cgcagccagc gcttacggtt
     2641 ttcttcgcca atgccgatat ccagatctgt agggctgata ttcatatcgc ccataatcag
     2701 taccggatta tcacgtttga gttcggtttc caggtagttt tgcagattct gataaaactg
     2761 cgcttttgcc gggaatttta tcggatggtc gcggctttca ccctgcggga agtaaccgtt
     2821 gatcacggtg acattaccca gcagtgaggg gatttccgcc ataataatcc gccgctgcgc
     2881 ctcttcgtcg tcaccgggaa agccgcgacg cacggcaatc ggcgtctctt tggtcagcag
     2941 cgccacgcca taatggcctt tctgcccgtg ataaaacacg ttgtagccga gcttcgccac
     3001 ctcttcgagc ggaaacatat catcatgaac ttttgtctcc tgcaggccaa tcacatccgg
     3061 ttggtgcttt tcgacgatgg cttcaagctg gtgaggtctg gcgcgcaggc cgttgatatt
     3121 aaaagagaca aatttcatag tcgctgccat ttattcagat aaagagtgcg tggatggtag
     3181 cagaatttcg cgttgcttac cgcctgttgt taagtgattt gatcgatggt tgttaatgat
     3241 gtcaacgatg gcgcaaaaaa tgcccgcttt tggtgcgcgc tgcgtcagaa tggcgcagta
     3301 atttccagta aatttcgtca atgatcacaa ttccagaatt atattttact tctgcataac
     3361 attgcgtttt ttattctttt tattcaccca catcgcaaac gtattcactt tatatgcact
     3421 ttaaatgcat atggtttggt tataactcct tgatttccag ttagcctccg ccgtttatgc
     3481 acttttatca ctggctggca cgaaccctgc aatctacatt tacagcgcaa acattactta
     3541 ttgttaacat ataaataacg aattatttac tgtagaggtc gctatgtctc agccaattac
     3601 gcgtgaaaac tttgatgaat ggatgatacc tgtttacgct ccggcaccct ttataccggt
     3661 acgtggcgaa ggttcgcgct tgtgggatca gcaggggaaa gagtatatcg acttcgcggg
     3721 tggcattgcg gtgaacgcgc tgggccatgc gcatccggaa ctgcgtgaag cgctgaacga
     3781 acaggcgagt aagttctggc ataccggcaa cggttacacc aacgagccgg tactgcgact
     3841 ggcgaaaaaa ttgatcgacg ccacgtttgc cgatcgcgtc ttcttttgta actccggtgc
     3901 ggaagccaac gaagcggcgc taaaactggc gcgtaaattc gctcacgacc gctacggcag
     3961 ccataagagc ggcatcgtgg cgttcaaaaa tgcgtttcat ggtcgcacgc tgtttactgt
     4021 cagtgcgggt gggcagccag cctattcaca ggattttgcg ccactgccgg cggatattcg
     4081 tcatgctgca tataacgata ttaactctgc cagcgcgctg attgacgact ctacctgtgc
     4141 ggtgattgtc gaacccatcc agggggaagg cggtgtggtg ccagccagca acgcgttttt
     4201 acaaggtctg cgtgaattgt gtaaccgcca caatgcgctg ttgatttttg atgaagtaca
     4261 aaccggcgtc gggcgcaccg gggaactgta tgcctatatg cactacggcg tgacgcctga
     4321 tctgttaact accgccaaag cgctgggcgg cggtttcccg gtcggtgcgt tgttggcaac
     4381 cgaagagtgc gcccgcgtga tgaccgttgg cactcatggc accacctatg gcggtaatcc
     4441 gctggcctcg gcggtggcag gcaaagtgct ggagctcatc aacacaccag agatgcttaa
     4501 tggcgttaaa cagcgtcacg actggtttgt tgagcgtctt aatactatta atcaccgcta
     4561 tggtttgttc agtgaagttc gcggcttagg tttgctgatt ggctgtgtac tgaatgccga
     4621 ttacgccggg caagcgaaac agatctctca ggaagcggcg aaagcaggcg tgatggtact
     4681 gattgcgggt ggcaacgtgg tgcgttttgc gcctgcgctc aatgtcagcg aagaagaggt
     4741 gacgaccgga ctggatcgct ttgcagctgc ttgcgaacac tttgttagcc gaggttcatc
     4801 atgatggtca tccgtcccgt tgagcgatca gatgtctcgg cgctgatgca gcttgccagc
     4861 aaaacgggcg gcggcctgac gtcgcttccc gccaatgaag ccacgctttc ggcgcgtatc
     4921 gaaagggcaa tcaaaacctg gcaaggcgaa ctgcccaaaa gtgagcaggg ctatgtgttc
     4981 gtgctggaag atagcgagac aggcaccgtg gcggggattt gtgccattga ggtggcggtt
     5041 gggctgaacg atccctggta caactatcgc gtcggcacgt tggttcacgc ctcaaaagag
     5101 ctgaatgtct ataacgcatt gccgacgctg tttctcagta acgatcacac cggcagcagc
     5161 gagctgtgca cgctgtttct cgacccggac tggcgcaaag agggcaacgg ctatttgctg
     5221 tcgaaatcgc gctttatgtt tatggcggct tttcgcgaca agtttaatga caaagtggtt
     5281 gctgaaatgc gcggggtgat tgacgaacac ggctattcac cgttctggca aagcctcggt
     5341 aaacgcttct tttcgatgga ttttagccgc gccgattttc tctgcggcac cgggcaaaag
     5401 gcatttattg cagaactgat gccgaaacat ccgatctata cccacttttt atcccaggaa
     5461 gcccaggacg tcatcggtca ggtacatccg caaaccgcgc ctgcccgcgc ggtgctggag
     5521 aaagaaggtt ttcgttaccg caactatatc gacatcttcg acggcgggcc aacgcttgag
     5581 tgtgacatcg accgcgtgcg cgccattcgt aaaagtcggc tggtggaagt ggcagaaggg
     5641 cagcctgcgc agggcgattt tccggcctgc ctggtcgcca atgaaaatta tcaccatttc
     5701 cgcgtggtgc tggcgcgtac cgatccggca accgagcgtt tgattttaac cgccgcacaa
     5761 ctggatgccc tcaaatgcca cgcaggggat cgcgttcgtc tggtgcgcct gtgcgcagag
     5821 gagaaaacag catgacttta tggattaacg gtgactggat aacgggccag ggcgcatcgc
     5881 gtgtgaagcg taatccggta tcgggcgagg tgttatggca aggcaatgat gccgatgccg
     5941 ctcaggtcgg gcaggcttgt cgcgcagccc gtgcggcgtt tccgcgctgg gcgcggctct
     6001 cattggctga acgtcaggtc gttgtcgaac gctttgccgg attgctggaa aggaataaag
     6061 gcgaattaac cgcgattatt gccagagaaa cgggtaagcc gcgctgggaa gcggcaaccg
     6121 aagtgacggc gatgatcaat aaaatcgcga tatcaattaa ggcgtatcac gttcgtaccg
     6181 gcgagcagcg tagtgaaatg ccggacggtg cggcgagcct gcgacatcgc ccgcacggcg
     6241 tgctggcggt gtttgggccg tataatttcc ctggtcattt gccgaacgga catatcgttc
     6301 cggcattgct ggcaggtaac accattatct ttaaacccag cgaactgaca ccgtggagtg
     6361 gcgaagcggt aatgcgttta tggcagcagg ctggcttgcc gccgggcgta cttaacctgg
     6421 tgcagggcgg gcgtgaaacg ggtcaggcgc tgagtgcgct ggaggatctc gacggtttgc
     6481 tgtttaccgg tagcgccaat accggctacc agctgcatcg ccagctctcc ggtcagccgg
     6541 agaaaattct cgcccttgag atgggcggta ataatccgct aattatcgat gaggtggcgg
     6601 atatcgacgc ggctgtccat ctgaccattc agtcggcgtt tgtcacagcc gggcaacgct
     6661 gcacctgcgc ccgccgttta ttgctcaaaa gcggagcgca gggcgatgcg tttcttgctc
     6721 gtctggttgc cgtcagccag cgattaacgc cgggcaactg ggatgacgaa ccgcagccgt
     6781 ttattggcgg gctgatttct gaacaggccg cacagcaggt ggttactgcc tggcagcaac
     6841 tggaagcgat gggcggacga accctgcttg cgccgcgctt attacaatca gagacatcgt
     6901 tgctgacgcc ggggatcatt gaaatgacag gcgttgctgg cgtaccagat gaagaggtgt
     6961 tcggaccgtt attgcgcgtc tggcgttatg attctttcga ggaagcg
//
LOCUS       DAMZET010000198         7003 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.198, whole genome shotgun sequence.
ACCESSION   DAMZET010000198 DAMZET010000000
VERSION     DAMZET010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.198"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..567)
                     /gene="motB"
                     /locus_tag="POB34_004180"
     CDS             complement(<1..567)
                     /gene="motB"
                     /locus_tag="POB34_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HDI5921033.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNR"
     gene            complement(564..1451)
                     /gene="motA"
                     /locus_tag="POB34_004181"
     CDS             complement(564..1451)
                     /gene="motA"
                     /locus_tag="POB34_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HDI5921034.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLATVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(1578..2156)
                     /gene="flhC"
                     /locus_tag="POB34_004182"
     CDS             complement(1578..2156)
                     /gene="flhC"
                     /locus_tag="POB34_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HDI5921035.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(2159..2509)
                     /gene="flhD"
                     /locus_tag="POB34_004183"
     CDS             complement(2159..2509)
                     /gene="flhD"
                     /locus_tag="POB34_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HDI5921036.1"
                     /translation="MHISELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            3289..3717
                     /gene="uspC"
                     /locus_tag="POB34_004184"
     CDS             3289..3717
                     /gene="uspC"
                     /locus_tag="POB34_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HDI5921037.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(3724..5148)
                     /gene="otsA"
                     /locus_tag="POB34_004185"
     CDS             complement(3724..5148)
                     /gene="otsA"
                     /locus_tag="POB34_004185"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HDI5921038.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(5123..5923)
                     /gene="otsB"
                     /locus_tag="POB34_004186"
     CDS             complement(5123..5923)
                     /gene="otsB"
                     /locus_tag="POB34_004186"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HDI5921039.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(6090..>7003)
                     /gene="araH"
                     /locus_tag="POB34_004187"
     CDS             complement(6090..>7003)
                     /gene="araH"
                     /locus_tag="POB34_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HDI5921040.1"
                     /translation="GMLVVFAVLFIACAIFVPNFATFINMKGLGLAISMSGMVACGML
                     FCLASGDFDLSVASVIACAGVTTAVVINLTESLWIGVAAGLLLGILCGLVNGFVIAKL
                     KINALITTLATMQIVRGLAYIISDGKAVGIEDESFFALGYANWFGLPAPIWLTVACLI
                     IFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVVRTKIIIFVLSGLVSAIAGIILASRM
                     TSGQPMTSIGYELIVISACVLGGVSLKGGIGKISYVVAGILILGTVENAMNLLNISPF
                     AQYVVRGLILLAAVIFDRYKQKAKRTV"
BASE COUNT         1849 a         1755 c         1626 g         1773 t
ORIGIN      
        1 acggttggga ataccgttca gtacaggcgc aatggcgcgc agaatgtcgc gcatataggg
       61 ttcgacatcg gcactgccgg ttctaaacat cgggcgattc tggctatcga tgatctgaat
      121 acgtagacct tcctggacca gatcgatttt gagatgggga cgtaacgccc gcagtttcgg
      181 atcggactct atcaactggt cgagatcacc ccgcaatttc cgcaatcgac tttgctccat
      241 gcgttttttc agctcttcga tgttcggctg cttattcact tccccctggc tttgggtgta
      301 atcatcacca ccgccgggaa ttgggctttc actattagaa atgcgatcgc cgcccgtaac
      361 cgcagtcgcc agtggagtcc ggaagtactc cgcaatctga atcagctctt ttgggctgga
      421 gatggagatc agccacatca ccagaaaaaa ggccatcatc gcagtcataa agtcggcata
      481 agcaatcttc cacgatccat gtgctgcccc gtggcttttg gctttgcgtc gtttgacgac
      541 aataatcgga tgcgcttgat tcttcatgct tcctcggttg tcgtctgttg ttgcggattt
      601 ttcaccgcac gcacatgctc ttccagttca ataaacgacg gacgttcgct ggaatagagc
      661 gttttgcgac caaactcaac ggcgataggc ggtgcgtaac cgttcagatt agaaagcaga
      721 gtgactttga cgcactgcat cattttgctg gtttcggcgc ttttctgacg taaaacagtc
      781 gctaatgggg aaataaatcc gtaagccaat aaaatgccga ggaaagtccc caccatcgca
      841 tgtgcgataa gcgcacccag ctcggcggca ggacgatcgg ctgaacctaa cgcgtgaacg
      901 acccccatta cagccgcgac aataccaaac gccggaagtg agtccccgac cagcgccaga
      961 ctgttcgccg ggacttctgc ctcgctttcg tgcgtctcaa tctcttcatc catcagggct
     1021 tcgatttcga aggtgttcat gtgaccgctg ataatcaggc gcagataatc gacgataaaa
     1081 tcaagcatga cgctatccgc gaggatgcgt gggtagctgg cgaagatctc gctctcacgg
     1141 ggattttcaa tatcacgttc cagcgaaaac atccccatct gccgcgattt tgccatcaac
     1201 cgataaagca gagccagcag atccatatac attgctttgg tgtatttgga gcgacgaaac
     1261 agcaacggca gcgccttcag cgtgccttta atcgctttgc cattattgcc gacgataaac
     1321 gacccaatcc ctgcaccggc aataatcacc agttcagcgg gttgatagag tgctccaagg
     1381 cttccaccgg tcatcaaata accgccgaaa actgtaccga gaacaaccag gtaacctaat
     1441 aagataagca cgacatcatc cttccactgt tgaccatgac aggatgttca gtcgtcaggc
     1501 gttaacgcgc gattggggca aaaaaaagca gcggtacgtc gttaccgctg ctggaatgtt
     1561 gcgcctcacc gtatcagtta aacagcctgt actctctgtt catccagcag ttgtgggata
     1621 atatcggcag gattctggga aagtttacgt ctttttactg cccgggatgg cggttgacat
     1681 aagctgcagg caaagctgcc aacaggctgg tgagcgtggg taataaaatt gccgccgcag
     1741 cagttgcagc tggaaagttg cagtaatcca ctttcaacaa accgcaccaa tgtccaggca
     1801 cgagttaatg ccagcagtgg tccttcttct gcttgtgggc actgttcaag gtataaacgg
     1861 taggctttga tcaccgcatc gacgccatta cacaaaccgg ttttcagtaa aaactgccat
     1921 gcattacaga acatcgaagc atgaacgttt tgttcccagg tcataaacca gtcggttgag
     1981 aatggcagca tgcctttcgg cggtgggctt ccgcgcagtt ctttataaag ttttatcagg
     2041 cgtccgcgac ttaactgtgt ttcgctttcc agcatctgca aacgagcgcc cagggtgatc
     2101 aattccattg ccagctgaat atcccgcgct tcctgaacaa tgcttttttc actcatgatc
     2161 aggccctttt cttgcgcagc gcttcttcag gctgattaac atcattcagc aagcgtgttg
     2221 agagcatgat cccggtatga atttgctgga gatcgtcaac gcgggaatct tgcgtcaact
     2281 gagtaatcgt ctggtggctg tcaaaacgga agtgacaaac cagctgattg gtttctgcca
     2341 gcttaaccat ttgcggaaga gtcagtgccg ctaatgttgt cgccatttct tcatttatgc
     2401 cgagacgaaa catagcggac gctttgtcct gaacaatcag acgctgtgca agtagtaaat
     2461 atgacaagtt gatgtcataa atgtgtttca gcaactcgga gatatgcatt attcccaccc
     2521 agaataacca actttatttt tatgcggtct caccgcaccc cgtgatgtcg ccgggaagcc
     2581 ccggtaaaaa ataattagca ttagaatagt tgcgataagc tgcaataagc agaaccacct
     2641 ttttggttta ataagtcctt acaaatagaa aggggtcctt acacttatct aagatttttc
     2701 ctaaatcgac gcaactgtac tcgtcactac acgcacatac aacggagggg ggctgcgatt
     2761 ttcaataatg tgtgatgcag atcacacaaa acactcaatt acttaacata aatgtataag
     2821 tcatactttt gttttgggtg tatttcaatc tgttaaaaag tttttcgcta cgctagcaag
     2881 caaaaatgaa acaggaataa tcgaaatggg atgttgcgca cagttaaaat aactcaccgt
     2941 aaataatcat ctgctataaa taatcacttt catgcaatac cagataagct atttttaaac
     3001 agacacttac cgcacaacaa actaattaaa tacaaagaaa ggcaacaatc aacttaatgt
     3061 tgatgaaatg ttttacatat atcaaccact tgtttaatgc ggagaatcag tacactattt
     3121 tcttataatt acatttgaaa ttatatgatc accgggagtg attgattaat tgtctttact
     3181 aataatcaga ctaatattta cctgtttgac cgagttggga ttgcgtcgtt tctccattag
     3241 gagtaaagct ttaatgtcac ctgaagttca cagaataagg aacaggttat gagctatagc
     3301 aatattcttg tcgctgttgc ggttacaccg gaaagtcagc aactgctggc aaaagcggta
     3361 tctatcgcca ggccagtaaa gggacacatc agtttaatta ctctcgcttc cgacccggaa
     3421 atgtacaatc aattagctgc gccgatgctt gaagatttac gcagcgtgat gcaggaagaa
     3481 acgcaaagct ttcttgataa gttaattcag gatgcggggt atcccgttga caagacgttt
     3541 attgcctacg gtgagttaag cgaacatatt ctggaagtat gtcacaaaca tcatttcgat
     3601 ttggtgattt gcggtaatca caatcacagt ttcttttcgc gagcatcctg ctcggcgaaa
     3661 agagtgattg cctcaagtga ggtcgatgtg ctgttagttc cacttacggg tgattaaccg
     3721 ctcctacgca agctttggaa aggtagcaac tttatcgcgc tgctggcttt ccgcgcttcg
     3781 cggaactatc tgctttaggt cgctaatgaa gcactcctgc cagtggttaa tatcgttttt
     3841 cacgataacg tccagcattt ctgcatgacg ggaaatacgt tccgccagcg acatagtcaa
     3901 tgcacgatcc agcgcagctg caacttcgtc acgatcgtag gggttaacaa ttaacgccga
     3961 cgttaactcg tttgccgctc ccgcaaattg cgaaagaaca agaacgcccg ggttggctgg
     4021 gtcctgagca gcaacatact cttttgctac caggttcatc ccgtcacgca gtggcgtcac
     4081 taagcccacg tcagagtagc ggaatatttt catcagtaat ttacggtcaa aatgctgatt
     4141 caaataataa agcggcgtcc agcctaattg cccgtattta ccattaattc gtccagcttc
     4201 attttcaagc tgatgacgaa tatcctgata ggcttgcaca tcaccacgcg acgttggtgc
     4261 aatctgggta taacgaattt taccatgatg ctgcggatat ttttccagca acgcttcata
     4321 ggcgagaaaa cgctctggca aacctttgga ataatccagc cgttcgacag aaaagatatt
     4381 ttgtacgttt ttcagttccg ctttaagttg cgccagtttt ggcggcagtg gcccggcagc
     4441 ctgtttggct atttctttcg gttcaatgcc gatcgggtag acttctgttc gaaatgcttt
     4501 gccccaggct gtatggcttt ttgcgctacg tgtcgtgacg cgggtcaggt tagaaagaca
     4561 atccaggaac gccagacgat cgttttctgt ctggaaaccc agcaaatcat aatcacaaag
     4621 ctgttcaagc aaggtgtcat atgtcggcag cgcgttgaag atttccggtg tcgggaaagg
     4681 aatatgcaga aagaaaccaa tgcgattatt cactccccgt ttgcgtaatt catgcgcaaa
     4741 tggcaacagg tgataatcgt ggatccagat aatgtcatcg tcttgcaaca gcggcagtaa
     4801 tttatctgcc agcaacgcat ttacgcgtag atagccgtcc caggcaggac gctgaaattg
     4861 caccagatcg agccgataat gaaaagcggg ccagagaacg gcattggaga attggttgta
     4921 gtattcgtca aggtcctgtt cgctgaggtt aaaagaggcc cacgtaatgt tacctttttt
     4981 cacctttttt agcggctgat cctcattccc tgtttcacca ctccagccaa accacagtcc
     5041 gcctgcggct ttcagtgccc ccagtatgcc aacggcaagg ccaccggcac tggcggcgtg
     5101 ctcgtctggt ggtgcaatcc ggttagatac tacgactaaa cgactcatag tcatcactcc
     5161 tgttattttc tcttttttgt tgtaatgcgg tggttatcat ttcaagccag ctccagacat
     5221 ccggcacacc cgccagtcgc catgatgcct gagttgcacc tgtgccaatt ttcactgaca
     5281 ttccgcccag tcggttaacg actgcgaagc cagattcatc ggttaaatca tcgcccagaa
     5341 atacgggcgt tcgcccgata aagggagctt cctgcataaa agctgcaatt gcctcacctt
     5401 tactggtacc tctcggtttg atctcgacaa cacactttcc ctgctgtaac gccatttgtg
     5461 gccagatctg agtaatacgt tgcgctaatg tcattaatgc gtcttcatgc tgcggagcct
     5521 gacgataatg cagcgcaaaa gccatccctt tcgcctccag ctccgcgccg ggatactgag
     5581 cgatgactgt atgcagttgc acgctaatat cacgcgcaat cgcatccggc agatgaacga
     5641 tatgcgtttt accattgatg tcacggcgct ccgccccatg cacgcccgct aacgggaagc
     5701 gataaggttt tgccagtgcg tcaagctcca ccattgagcg ccctgatatc aatgccaatg
     5761 caccatcact tgcggttgcc agtagctgta gtccttgcag aatgttgtca ggcacgacga
     5821 cctgatcggg atgcggtttg atttccgcca gcgttccatc aagatcaaaa aaccaggcat
     5881 atttcgcgga tagttcaggg gtttcggtta acggttctgt cacccggctc tcctccttct
     5941 cttttcttat tgttttgcgc agaacgcgct ttacagacat gtaagtatag acagtgtgac
     6001 gggggtcgcc atttgcaatg aagaacagcc aaccgttgtg gcggctggct gtggtgggaa
     6061 aaaacgttaa attgttgtgg aaaaaagcat cagacagtgc gtttcgcttt ttgcttgtaa
     6121 cggtcgaaga tcaccgctgc cagcaggatt aagccgcgaa ccacgtactg cgcgaaagga
     6181 gaaatattaa gcaggttcat ggcgttttcc acggtgccta aaattaagat acccgccacc
     6241 acatatgaga tttttccgat gccacctttc agagaaacgc cacctaaaac gcaggcggag
     6301 ataacaataa gctcataacc aatcgacgtc attggctgcc cactagtcat acgtgaagcc
     6361 agaataattc cggctatcgc agataccagg ccagagagaa caaagataat aattttggtg
     6421 cgaacaaccg gtacacccgc cagacgcgca gcctcttcgt tcccgccaat cgccagggtg
     6481 ttacgaccaa aggtggtttt attcagcaac aaaccaaaga taatcagaca cgcgacggtg
     6541 agccagattg gcgcaggcag accgaaccag ttagcgtaac caagggcaaa gaagctttca
     6601 tcttcgatac cgaccgcttt accgtctgaa atgatgtacg ccagacctcg aacaatctgc
     6661 atcgttgcca gtgttgtgat cagagcattt attttcagtt tggcgataac aaagccattg
     6721 accaggccac agagaatgcc aagcagcaac cccgctgcca cgccaatcca caggctttca
     6781 gtcaggttga taaccaccgc cgtggtgaca cccgcacagg caattacgga ggcgacagaa
     6841 aggtcaaagt caccggaagc aaggcagaac aacatgccac acgccaccat ccccgacatg
     6901 gaaattgcca ggcccaaccc tttcatatta atgaaggtgg caaaatttgg gacaaaaatg
     6961 gcacaggcga taaagagcac cgcaaacacc accagcatgc cgt
//
LOCUS       DAMZET010000199         6967 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.199, whole genome shotgun sequence.
ACCESSION   DAMZET010000199 DAMZET010000000
VERSION     DAMZET010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.199"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(59..1651)
                     /locus_tag="POB34_004188"
     CDS             complement(59..1651)
                     /locus_tag="POB34_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HDI5921041.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            1870..2790
                     /gene="ldtB"
                     /locus_tag="POB34_004189"
     CDS             1870..2790
                     /gene="ldtB"
                     /locus_tag="POB34_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDI5921042.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(2849..3967)
                     /locus_tag="POB34_004190"
     CDS             complement(2849..3967)
                     /locus_tag="POB34_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HDI5921043.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(3964..4431)
                     /gene="mntR"
                     /locus_tag="POB34_004191"
     CDS             complement(3964..4431)
                     /gene="mntR"
                     /locus_tag="POB34_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HDI5921044.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            4617..4745
                     /gene="mntS"
                     /locus_tag="POB34_004192"
     CDS             4617..4745
                     /gene="mntS"
                     /locus_tag="POB34_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HDI5921045.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            5017..6600
                     /gene="opgE"
                     /locus_tag="POB34_004193"
     CDS             5017..6600
                     /gene="opgE"
                     /locus_tag="POB34_004193"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDI5921046.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(6649..>6967)
                     /gene="ompX"
                     /locus_tag="POB34_004194"
     CDS             complement(6649..>6967)
                     /gene="ompX"
                     /locus_tag="POB34_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HDI5921047.1"
                     /translation="TYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASIYGVVGVGY
                     GKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDVGTWIAGVG
                     YRF"
BASE COUNT         1755 a         1886 c         1654 g         1672 t
ORIGIN      
        1 ttttgcattt gtcatcagcc gccgtcaccc cgacagtatc ggggtgacgg ataatcactt
       61 actcgatccc tttactacgc aggtaatctt cgtaattacc gctaaagtcg atcacgcgtt
      121 ccggggtgat ttccagaatg cgggtcgcca gggagcttac gaactcacgg tcgtgtgaaa
      181 caaagatcag cgtgccctga tacagttcca gcgccatgtt cagtgactcg atggattcca
      241 tatccaggtg gttggtcggt tcgtccatga tcagaatgtt cggcttctgc atcattaact
      301 taccaaacag catccgccct ttttccccac cggaaagcac tttagctggc tttttgatgt
      361 cgtcctggct gaacagcaaa cgaccgagaa tgctgcgtac cgcctgctcg tcatcgcctt
      421 cctgcttcca ctgactcatc cattcgaaca cggtcagatc attttcaaac tcatattcgt
      481 ggtcctgggc gtagtaacca atgcgcgcgt tttcagacca ttttacggtg ccgctgtccg
      541 gttgcagatc gcccaccagc gttttcagca gcgttgattt accgacgccg ttggtaccca
      601 gtaccgcaag tttttcaccg acctccagca gcaggttgag atttttaaac agtggaccgt
      661 tatcaaaccc tttggtcaga ccttccactt ccagcgcgtt acggaacagt ttcttatcct
      721 gttcaaaacg gatgaacggg ttctgacggc tggaggcttt cacctcttcc agtttgattt
      781 tatcaatctg gcgcgcgcgc gaagttgcct ggcgagattt cgaggcgttg gcgctaaagc
      841 ggctaacgaa agattgcaac tcagcaatct gcgctttctt cttggcgtta tcggccagca
      901 gacgttcacg cgcctgggtc gccgccgtca tgtactcatc gtagttaccc ggataaacgc
      961 gcagctcgcc gtaatccaga tccgccatgt gcgtacagac catgttaagg aagtgacggt
     1021 cgtgcgagat gatgatcatg gtgctgtcac gctcgttcag cacctgttcc agccagcgaa
     1081 tggtgtcgat gtcgaggttg ttggtcggtt cgtcgagcag gagaatatcc ggatcagcaa
     1141 acagcgcctg cgccagaagc acacgcagct tccagccagg agcaacttca ctcatcgggc
     1201 cgtagtgctg ttccactgga attcccacgc caagcaacaa ttcaccggcg cgagcttccg
     1261 cagagtaacc gtccatttcg ccgtatttaa cttccagatc ggccacttta tagccgtctt
     1321 cctcactcat ttccggcaaa gcatagatgc ggtcacgctc ctgcttcact tcccacaact
     1381 ctttatgccc catgatcacc gtatccagca cagtgaactc ttcaaaggca aactgatcct
     1441 gacgcagctt accaatgcgc tcgttgggat cgagggaaac gttacccaaa gtcggctcta
     1501 agtcgccgcc gaggatcttc ataaaggtgg atttaccact accgttcgcg ccaatcaggc
     1561 cgtaacggtt gccgccgcca aatttgacgg aaatgttttc aaacaacggc ttactgccga
     1621 actgcatggt gacgttactg gaaactaaca caggcgtatc ctgaaaagag atatgacaaa
     1681 ccgcgcatta tgccataagt ctgaaagaag atcctgctcc tcgcttaagg tctacactca
     1741 agccaaaggc aaaaaatgtg atttcgtaca catctgattt cactgtgagc tggaatgaac
     1801 ttataatgcg cttccaatac tctaatattc tcaatccaat ggcctgccag gcacaaaatc
     1861 tcgcttaaca tgaatatgaa attgaaaaca ttattcgcag cggccttcgc tgttgtcggc
     1921 ttttgcagta ccgcctctgc ggtaacttat cctctgccaa ccgacgggag tcgcctggtt
     1981 ggtcagaatc aggtgatcac cattcctgaa ggtaacactc agccgctgga gtattttgcc
     2041 gcggagtacc agatggggct ttccaatatg atggaagcga acccgggtgt ggataccttc
     2101 ctgccgaaag gcggtactgt actgaacatt ccgcagcagc tgatcctgcc ggataccgtt
     2161 catgaaggca tcgtcattaa cagtgcagag atgcgtctgt attactatcc gaaagggacc
     2221 aacaccgtta tcgtgctgcc gatcggcatt ggtcagttag gcaaagatac gcctatcaac
     2281 tggaccacca aagttgagcg taagaaagca ggcccgacct ggacgccgac cgccaaaatg
     2341 cacgcagagt accgcgctgc gggcgaaccg cttccggctg tcgttccggc aggtccggat
     2401 aacccgatgg ggctgtatgc actctacatc ggtcgcctgt acgctatcca tggcaccaac
     2461 gccaacttcg gtatcggcct gcgtgtaagt catggttgtg tgcgtctgcg taacgaagac
     2521 atcaaattcc tgttcgagaa agtaccggtc ggtacccgcg tacagtttat tgatgagccg
     2581 gtaaaagcga ccaccgagcc agacggcagc cgttatattg aagtccataa cccgctgtct
     2641 accaccgaag cccagtttga aggtcaggaa attgtgccaa ttaccctgac gaagagcgtg
     2701 caaacagtga ccggtcagcc agatgttgac caggttgttc ttgatgaagc gattaaaaac
     2761 cgctccggga tgccggttcg tctgaattaa tcttcaaaac ttaaagcaaa aggcggactc
     2821 ataatccgcc ttttttattt gccagaccct agttggccgg gagtataact aacaaaacat
     2881 atcccaccaa cgccgcccac aacagcatcg gtattgaata gagatggaaa cgccaccaga
     2941 tgcggcgatc gttcgccata cgtagcgcaa tcaaatttgc cagcgatccg ggtaataacc
     3001 caaagccacc tacgtttacc gcccatacca gtaataaaga cggcggcaca tagttcagca
     3061 acaatatggt actcggcacg ttactgatca cctgcgataa accgattgcc gttaaccata
     3121 acccgggttc agatagatga ctcacgttac ccaacacgcc ttgcaacgct ggaagctggg
     3181 tcagtaaatg gacgtcgata aacatcgcca taaacaccag cagcagcgtc cagtccacac
     3241 tgagcaccac gcgacgtgcc agcagcgcaa agcctgccgc cacaatcacc agtccccaca
     3301 gctcttgttt gaactccagc gccgtcagaa agacgatata cagccccaga caactccaca
     3361 ccagccgcgg tttccactcc ggtgtttgca cccccgtatg gtattgcagc gcctttccag
     3421 ggaaacaaca ccagcacagg agcaggagcg tcagcatcat tgcgccagcc agcggtgcca
     3481 tttgggcaat aaatccggca aacgaaagac cagaacgtcc ccagataaga atattttgcg
     3541 ggttgccaat tggcgtcagt agcgaaccag cgttgactgc cagcgcctca aaaataatca
     3601 gccgattaac cgggatctca cacagtcttt ttagcgtgat agtcagcgga acaacaataa
     3661 acagcgcgac atcgttggtc agaaaggtag aaagcagcgc cgccgccagc accataaaca
     3721 tcgccagccg acgctccgta gcaaagcggc gcaccatttt gcgccccagc acatcaaaat
     3781 aaccgcttaa ctccacacct ttggtcagca gcatcaggcc gcttaaggtg atgatggtgt
     3841 gccagtcgat agcagcaggc caggatttcg gtgcaaaggg cacgaaaaag cttaatccga
     3901 taccaacaag aattaataac tgaaaaaaac gatcgccttg cagcgtgcgt aaaaaaggca
     3961 ggctcatttg gcaccgtgtt tctgggtaaa caaacgaaaa gcgtcgagcg tctcttcact
     4021 aacatggtgc tccatgcctt ccgcgtcgcg acgggcgatt tccggactga cgcccaacac
     4081 caacaagaaa ttttcgacta tctgatgtcg ctcgcggctt tcctgcgcca gcttctctcc
     4141 ttctgccgtt aaaaacacgc cacgccaggg gatcatttca atcagcccca tggttgccag
     4201 ccgcttaagc attttagcca ccgtcggttg cgaaactccc agacgagcag ccatgtccac
     4261 ctgacgagct tcccccactt ccctgatcaa gtcagaaatc agctcaacgt aatcatcaat
     4321 aagctcgcgc cgatgcgcct ctctgacctg gcggaaccct tcaacgtgct cttccacgtt
     4381 cactaactgc gtcacttttt ttgctgttgg cgtacctgcg cgacgactca ttgtgcttcc
     4441 tcattatggt gacgcataca tagcgttctc atttcgtgcg cacattgtaa accagagttg
     4501 cgaaggtaca aaaaattaac gttttagcaa tagctatata atatagcctg tgctatatct
     4561 gtatgtaatg caatcatccc tcaaggatcg acgggattag caagtcagga ggtcttatga
     4621 atgagttcaa gaggtgtatg cgcgtgttta gtcattctcc ctttaaagta cggttaatgc
     4681 tgctctctat gttgtgcgat atggtcaaca acaaaccgca gcaagataaa ccttccgata
     4741 aatagcggcg tcgcggtacg ccgcttcact cctgctttca tgcaggcata acgcgttttg
     4801 gtctgaaaaa ccccactttt tgtcggattt gcaatcccct tcgcaaaaga tttgttcgtc
     4861 agtagttgac ctgaacggcg gctcgctcta tcttcttgca gccctgcgta tattgcggct
     4921 cgcggatgcg gacccctttc cactcttcac gcactcttgc aggtattgac ccttgacgcc
     4981 agggtaagca catggcgttt gttacgatag tggcatatga atttaaccct caaagaatcg
     5041 cttgttaccc gtagccgggt atttagcccg tggactgcgt tctacttttt acagtcgcta
     5101 ttaattaacc tcggcttagg ttaccccttc agtttgctct acaccgctgc gtttacggct
     5161 attttgcttt tgctatggcg aacattgcct cgcgtacaaa aagttctggt cggtgtcagt
     5221 tcgctggtgg cggcttgtta tttccctttt gctcaggcct acggcgcgcc taatttcaat
     5281 acattgctgg cattgcactc caccaatatg gaagagtcga ccgaaatcct gacgattttt
     5341 ccgtggtaca gctacctggt cggcttattt atttttgcgc tcggcgtaat agcaatcagg
     5401 cgaaaaaaag agaatgaaaa agcgcgctgg aataccttcg acagcctgtg tctggtattc
     5461 agtgtggcga cattttttgt tgctcccgtg caaaacctgg cctggggtgg cgtatttaaa
     5521 ctgaaagata ctggctatcc ggtatttcgt tttgctaagg atgtcatcgt caataataac
     5581 gaggtgattg aagagcaaga acggatggca aaactttccg gaatgaaaga tacctggacg
     5641 gtcactgccg ttaagccgaa gtatcagacc tatgtggtgg tgatcggtga aagcgcgcgt
     5701 cgcgatgccc tcggtgcctt tggcggtcac tgggacaata ccccgtttgc cagcagcgtt
     5761 aacggtttga tatttgctga ctacattgcc gccagtggct ccacgcagaa atcgcttggc
     5821 ttaacgctca atcgcgtagt cgatggcaaa ccacagtttc aggataactt tgtcaccctg
     5881 gcaaatcgcg cgggcttcca gacctggtgg ttttccaacc agggccaaat cggcgaatac
     5941 gatactgcta tcgccagcat cgccaaacgt gcagatgaag tgtacttcct gaaagaaggt
     6001 aattttgaag cagataaaaa cacgaaagac gaagcgttac tggatatgac cgctcaagtg
     6061 ctggcgcaag agcactcgca accgcagctg attgttctgc atctgatggg ctcgcatccg
     6121 caggcctgcg acaggacaca aggcaaatac gaaacctttg tgcaatcgaa agaaacgtcg
     6181 tgctatctct ataccatgac gcaaacggac gatttactgc gcaagctgta cgatcagtta
     6241 cgcaacagcg gcagcagctt ctctctggtt tacttttctg accacggtct ggcctttaaa
     6301 gagcgcggca aagacgtgca ataccttgcc catgatgata aatatcagca aaatttccag
     6361 gtgcctttta tggtcatttc cagcgacgat aaagcgcatc gggtgattaa agcccgccgc
     6421 tcagccaatg acttcttagg ctttttctcg cagtggacgg gaattaaagc gaaggaaata
     6481 aatatcaaat acccgtttat atctgagaag aaagccgggc cgatatacat caccaacttc
     6541 cagttacaga aggtggatta caaccatctc ggaaccgata ttttcgaccc gaaaccttaa
     6601 aaacaaaaat ccgccccgag aggcggattt tttatatcac caaagtgatt agaagcggta
     6661 accaacaccg gcaatccagg tgcctacgtc aacgctacga atacggctct gctcgtaaga
     6721 gaagtccaga gcaacgtttt ccatcgggtt gaactgcaga ccagcaccgt aggagaaacc
     6781 gtagtcgctg gtgtcgtgtt tgtaggtcgg gtattcagtg gtctggaatt taccataacc
     6841 cacacccact acaccgtaga tgcttgccca gtcgttaatg cggtaagccg gaccagcagt
     6901 gatgccgtag tactggtttt tgttgtagtc accagagctt gcagtacggc ttttctcggt
     6961 gtaagtg
//
LOCUS       DAMZET010000200         6962 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.200, whole genome shotgun sequence.
ACCESSION   DAMZET010000200 DAMZET010000000
VERSION     DAMZET010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.200"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(126..335)
                     /locus_tag="POB34_004195"
     CDS             complement(126..335)
                     /locus_tag="POB34_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921048.1"
                     /translation="MARRELELREIPYIKNSLHANYSYKSISIGSKQGWLISAKLKVP
                     ETFEPDMIFIEISDPEGFINIPDVL"
     gene            complement(374..5236)
                     /locus_tag="POB34_004196"
                     /pseudo
     CDS             complement(374..5236)
                     /locus_tag="POB34_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
     gene            complement(5256..5717)
                     /locus_tag="POB34_004197"
     CDS             complement(5256..5717)
                     /locus_tag="POB34_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HDI5921049.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5745..>6962)
                     /gene="tssI"
                     /locus_tag="POB34_004198"
     CDS             complement(5745..>6962)
                     /gene="tssI"
                     /locus_tag="POB34_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDI5921050.1"
                     /translation="DYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKSAHGQNF
                     ARWQMDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQA
                     VPGRQGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRV
                     RVKFNWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIM
                     GRTYHQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTE
                     VLNDRTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGT
                     IAGSVHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDGIQLQVGESTVIRLSK
                     DGITIVGGSVFIN"
BASE COUNT         1302 a         2121 c         1813 g         1726 t
ORIGIN      
        1 actatttcca tgatcttgcc ttgcgttcat ccaggcagga atatccctgt tagcagcctc
       61 tgtgcgatgt acgccagcgg tctgtaaaga aaccgattac cttataacaa aacctctaac
      121 gtagtttata atacatcagg aatattaata aaaccttctg ggtcagatat ctcaataaat
      181 atcatatcag gctcaaaagt ttcagggact tttaatttcg ctgaaattaa ccaaccttgc
      241 ttagaaccaa tactaataga tttatagctg taattggcat gtagactatt tttaatataa
      301 ggaatttccc ttaactctaa ctcacgccga gccaagtcaa gagccatttc gatagttatt
      361 ttgttatgtt ttatcaattt attagtccca tcatgaattt ttctctcaat ccctcaacat
      421 attttatggc atgagctatt tccgcttcat tgaataaatt tttacttttt aatatttcat
      481 ttagaacgtg ttcctcccta gataaacgtc ctagtgcttc gtatgcttct cgtccaattt
      541 tcgcaaattg cttagcatga atcacttcat gatacacctc atataaggtg gcatttttcc
      601 taagataaat attgcgaagg ccataatcaa aagctgccct agcatttggt ggcaaatatt
      661 tatctgcttt ccttattact ttaattccta gcttctccat ttctttagca atattgttta
      721 tttgactacg tgataaacga accccatcca ggaaacccgt atgcccacta acacgacgac
      781 catcgccaat atagtccatc aaactgctga ttttagaact tgcagccaat cccagcggat
      841 caacccaccc acacggattt ggcacatacc ggtaagtctg ctcacccccc agcaacccca
      901 gcggatcact caccaggtac atcccggttt ccgactcgta ataccggaac cggttatagc
      961 acagcccgga ttccgcatcc tgccactgtc ctgcatacag caggccgggg gcggcttccg
     1021 ggtccagttc cccgcgcggg tccgggtagc cggtgtctgc gggcaggctg agtgccagcc
     1081 cccacaggct ggtctgcccc ggtcgccaga cggttttacc ttcactgtta aagagcatca
     1141 gcggacggcc cgtcaggtcg ctcaccgcat gattcgtccg cgtcacccac tgcggggcca
     1201 ccgacggatg cggctgccgt acccggctga actgctggct tatcagctca aagccgttaa
     1261 acaccaggtg ccgtacgctg tacagtttat catcgcggta ttcccggatt tccgcaatac
     1321 tgtcgccgtc ccacagatac gtgaagcgga ttttcttccg gtcgcagcgt ttctctgttc
     1381 gtctgccgct ggcgtcgtgg cggtattccc acagctcacc ctgtgcgctg caatacccgg
     1441 tcagctggtc acggctgtcc caccggaacc gctctgtttc cggtcggtag ccgtcacggt
     1501 gtctggtgcg gctgaccatc cggcctgcgg cgtcatagtc atactgcgtg tttccggcct
     1561 gtttcagccg gtggcctccg gcatactggt cagtctcttc acttatccgg tgcctgcccg
     1621 cagactgcgc cttcaggtag ccgcagctgt catagccata ggactcatcg ccttcgccat
     1681 agcccagccc ggtgcctgag gctgtcaccg ccatgacctg gccgttccgg tccagccggt
     1741 actcccgccg tgtttcccgt ccgtaatctt cccggctggt gacagccgac acattacccg
     1801 cgctgtcgta acggtactcc cgtaccagtg agggaaccag cccgccccat gtggcttctt
     1861 ccggaaaaaa tccggcgcgc tggctgacag gctgaccggc tgcattccag ccctgttcac
     1921 aggcaaaacc ccggttactg tgccggagca cttcacgtcc cgcctcatca cggctgaacg
     1981 tcagcgtctc atcccccggc aggataacct ctgccagttc tccggcggcg ttgtaacggt
     2041 aggtggtttt cagcagggat tcatcgggat gtctgtcgcg ttcatcttcc ggtggcagga
     2101 tacagcggat gacggtgtgt tcatcggggt agtggtattc aatggcccgt cggccctgag
     2161 tggtgacatt cacccgcccg cgttcgtcat agccggtgcg ctgtgatatg ccgtccgggc
     2221 tgaagatacc cgtcagcctg ccggatttgt catacgcata ctgcgttttg ccttccggcg
     2281 tgatgatttc tgccaggcgg cctgcggcat cccggctgta gaggatggtt tcctccagac
     2341 cgttaacctt tgcggtatac aggccatcgg cgtcatagcg gtaacgccag accacgccgc
     2401 cccagtcatg ctcttccgtc acccggccgc agttatccag aacataaaga tatgactcac
     2461 cctgcgggtt gatgacttct gtcagttgca gcgtgtcttt atcgtagcgg tactgccagc
     2521 ggtggccttc accgtccgtt cgcgccaccg gcaaatcgaa cggaccgtat tccatcgtcc
     2581 agacaacgcc gttaccgtcc gtccaggcca gcaggttgcc gtggcggtca tagcggaatc
     2641 cttccgcgcc ggttccagca cgttcaatgc cactcagttt atggtgctgc tgccactgta
     2701 accgcactga ctgaccgctg gggccgcgca tcttcaccgg caggtcgtga ccgctgtatt
     2761 cggtgtggtg ttcacggccc atcacgtcgg tgagtgtttc cggccggttg tggtggtcgt
     2821 actctatgcc cagacgccgt tcatcctggc ggaatatccc cgacagcagc ccctgcggcg
     2881 tgtacgccag ccgggtctct tcgccctgtg gcgacaccag acttaccggc aggccgcgtt
     2941 catcacactg ctgcgtccac gtctgtccgg cagggtcggt gatgttgctg agcaggccgc
     3001 gctcatcata gcagtactgc cactccgcgc cgtctgccgc cgtgtaacgg cttaactgac
     3061 cgtgtgcggc gttgtattca aacttctcca cccgcccggc aggggagtga cgggccagca
     3121 ggtgatggaa ttcatcccat taatacgtgg tggtgctgcc gtccggcgct tcttcccgca
     3181 gaatattgcc gtcaggatca tagtaataac ggtgagtgcc cttgccgtcc gtcacggtgg
     3241 tgcagccgtc accatagtcc agcgtggcgt tgtaatagcc ctccgccccg gtgacggtca
     3301 cgcaccggcc ctgttcatcg taggtgaaac ggctccacgt ctggtcgtta tcggaccagc
     3361 ggatgatccg gtccgcagcg tcgtactcat aaaacaggtg gtagtccagc cgcgcatccg
     3421 cttctgtcag ccgcccccgc gcatcctgtt cgtaggtcgc cagcgtctgt atgccgttat
     3481 ccgtgcggtg aatggctttc aggtagccgc tggcgtggta catcagttta agccggatcc
     3541 cgtcggtgtg actgacaccc gtgatgcggt actggctgtc gcgctcaaac gtgatgcggt
     3601 tgctggcggt gtcgctcatc ccgcccagca tatacatccg gtcgtcgggg ctgtgtgcca
     3661 ggcggtcatc gctggtatcg tcagtcaccg ggtggcgcag acgcattccc ggaaaggcgt
     3721 cataaaagaa gcggctgctc agtgcatccc tgtcgaacag ctccaggtaa tcgtcgcggc
     3781 ggcgcagaat gtactgggcg tgatacgggt taaccgccgt atcactgtca ccgtcgaagg
     3841 cgaagttcag cgtggctccc tggctgataa tatctacccg gtttccctgt tcacgcaccc
     3901 aggcgtattc gctccaggag tcgctccagc ccacgccggt caggccatgc agcggcagta
     3961 cagaactgtg gaaacgggtg aaggagaggg ggagtgtctg gcccagggtg aagtcggtac
     4021 gttcgtcgca gtacgcgccg gttaccggat cgacaggatc tgttacccat ctgatgactt
     4081 tattggcaat actttttaac gacttacctg ttttccccag caacgcgccg agctttgccc
     4141 ccagcagctt cccgcttttc cccagcaggt tccccagttt gccgagtctc gccagattct
     4201 tcagttttcc cagcagtccc gctggcggca gaagatagct ggctatcgtt attcccccca
     4261 ggatcattct ctgccacggc gggaactccg gctggatatc cagataggtc acctgctcac
     4321 cgccaataaa gacgtccggc gaacctccca cgattgctgc gctgcactcc agtttgtcgc
     4381 cctggcgtgc ggcaggctga ttgttgataa agacgttgct gctgccctgg gctatcctcg
     4441 gtggtttgtt ctccttgtcg cagagcgccg tgtcctcacc ggcttccggg aaatccacat
     4501 cttttttgat ttcgccacgc ccgcccacca tggtggcaaa ctccattaat accggaccgg
     4561 cgggaaacag aagcgatgcg ccggcgctga cgcgatccat aaaaccggca ttgctgtccg
     4621 tcagggtgat aacagcgttc ccccaggcct tcgccagtgg tactgctttt tccgctgccg
     4681 attcaagcag gtcagtggca atatcacccc atgagggttc cccctgtggg acttcaggta
     4741 ttgctccagg tgggggagga gggggaaggg tgacggcggc cctggcagcg ggctttccgt
     4801 taatttttac gttttcagaa ccggtattga tggcgccgtc cgtgtcacaa aaactgttta
     4861 tccatgactc aagctggttc tgtttctcct ccacataatc actcgcctta tagctggcat
     4921 acatcagcgc aggcaccaga accagcgcat aagggccggt ggcacaggcg gccataatca
     4981 ggcaaccttc tgcgatggtc actatcgccc ccagcacggc ccctgccagc gcccccaaaa
     5041 aactcttgtg ctgaatttca tcgcccagcc tggcggcggg ggggccagac ttttttgatg
     5101 ccagcatctg tgcaatggtt ttgtcgttgg ctgcccgttt catctggctg gcgaccatcg
     5161 cctgctgtga cagcattcgc attgccagcg taccgcctgc ggtcgctccc tgtggcccgc
     5221 ctggtccttc actcatgtgt tatccctcgt ttatgtcagc gcggctggtc cttttggatg
     5281 tcattctccg gtggcaacgg ggtgaagctc tttatcacct ccagcagcat cgtcttttga
     5341 ctgttcttca gggataagga ggtgctgaaa gtgaaggtca gcagcgtatc tccacgccgc
     5401 tggaatacgg ctatctggct aatccgcccc tgattcccct catcttccgg accgttccag
     5461 gaataatcca gcagccaggc cggggcctct gccagagtga tttcccggca ggcatgttcg
     5521 gtgtacccat caagtttgcc ctggtacaac gccagcgttt cctggtacag ctctggtgtc
     5581 agggttccct gtggaacggc ttcgcgggta atgaccaggt tgatgccact gttatctgtg
     5641 gacaccagga cctgcattga ctgatcctgc cattgagacg gcagttgaat gtggccttca
     5701 ttaaaagtga actgcataaa atactccctg ataatctgag tgaatcagtt gatgaaaaca
     5761 gaaccgccca cgatggtgat gccatccttc gacagcctga ttaccgtcga ttccccgacc
     5821 tgtagctgaa tgccatccgg cgtcagcgca attttgcttt cctccacagc cagcaaaatt
     5881 tccttaatcg ccgtataagc ctgtataccg tcgccttcgc caccattgtt tacagcgtca
     5941 acatgcaccg aaccggcaat cgtgcctctg tcctgaaaga catcctcaga taaagaccct
     6001 ttcagtactc cggtgatctt gtggcctttt tccaccgtaa gaaggcggtt accttcctgg
     6061 atcgtgaccg tctggtcatt ttttacggtt tcgcggtgat cgtgttttac ggtggtggta
     6121 cggtcgttga gcacttcggt atccatgttc ttctgcgcgt ggatgtagac ctgttctctc
     6181 cctgtcgcat catcaaactt cagctcatta aatccgctgc ccatgtacgt tttggagcgg
     6241 atggtcatct gcgtctttgt tcccggcagg ctgccggggg tgcggttttc ctggtggtag
     6301 gtgcgcccca taatgatcgg ctggtccgga tcgccgttga ggaagtctac aatcacctcc
     6361 tgacccacac gcggtatcgc caggtggcca aaaccggtgc ctgcccacgc ctgtgccaca
     6421 cggatccagc acgaactgtc ttggtctgcc gggttataac ggtcccagtt gaacttcacc
     6481 cgcacgcgac catgttcgtc gcagaagatt tcctcgcctg ccggtcctgt cacgacagcg
     6541 ctctgcgggc cgtcgaccag cggtttcagc aacggctgtg gtcgccatgt tctgtctgcc
     6601 gggatcaccg caaaatggtt ctccagcgcc gtccccgctc cctgccgtcc tggcaccgcc
     6661 tgtggctgtt cgccgtgcag ttcacttgcc accacctgcc attcccggtt caggttcgcc
     6721 tgcggatgcc ccgtcagcac aattcgccgt cccggccata tctccggcga gcggctcatt
     6781 ccccgcgcgg tttctgcgtt gtttcgccag ccgtccatct gccagcgggc aaagttctgc
     6841 ccgtgggcgc tcttgaaacg tccggggtag tcatacactt catactgcgt gcgctggtag
     6901 tcctggtgct ggccttcctg ttcaaaacgt ccggcccagc cggggcgttt aaaggtgtag
     6961 tc
//
LOCUS       DAMZET010000201         6879 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.201, whole genome shotgun sequence.
ACCESSION   DAMZET010000201 DAMZET010000000
VERSION     DAMZET010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.201"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..784)
                     /gene="gsiC"
                     /locus_tag="POB34_004199"
     CDS             complement(<1..784)
                     /gene="gsiC"
                     /locus_tag="POB34_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HDI5921051.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRL"
     gene            complement(802..2340)
                     /gene="gsiB"
                     /locus_tag="POB34_004200"
     CDS             complement(802..2340)
                     /gene="gsiB"
                     /locus_tag="POB34_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HDI5921052.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKVQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(2360..4231)
                     /gene="gsiA"
                     /locus_tag="POB34_004201"
     CDS             complement(2360..4231)
                     /gene="gsiA"
                     /locus_tag="POB34_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HDI5921053.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(4218..5183)
                     /gene="iaaA"
                     /locus_tag="POB34_004202"
     CDS             complement(4218..5183)
                     /gene="iaaA"
                     /locus_tag="POB34_004202"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDI5921054.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            5387..6622
                     /gene="moeA"
                     /locus_tag="POB34_004203"
     CDS             5387..6622
                     /gene="moeA"
                     /locus_tag="POB34_004203"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDI5921055.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            6622..>6879
                     /locus_tag="POB34_004204"
     CDS             6622..>6879
                     /locus_tag="POB34_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HDI5921056.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPK"
BASE COUNT         1569 a         1987 c         1749 g         1574 t
ORIGIN      
        1 gtaagcgtcc aagtccgggc cagttgaaaa ctttctccac cacgatagaa ccaccgagca
       61 aaaagccaaa ctgtaagccc atcatggtca ctaccgggat catcgcgtta cgtagcccgt
      121 gtttgaggac aacccaggtt tcgctcaccc ctttcgccct cgcggtacgc atataatctt
      181 cgcttaaaac atcgacaaac gacgcgcggg taaagcgcgc catcacggct gccactgccg
      241 cgccgagcgt cagggagggt aaaatgtagt gctgccaact gtctgctccc acggtaggca
      301 gccagcccag ttcaacggag aatacctgaa ttaaaagcat ccccagcgca aatgccggaa
      361 acgagatccc cgacaccgca atggtcatac tcaatcgatc cggccaacga ttacgccaga
      421 cggcggcgat aattcccgcc gccataccaa atatcaccgc ccagaccata ctggttatgg
      481 tcagccacag cgttggcata aagcggctgg caatctcatc ggcaaccgga cgacgcgaca
      541 ccatcgacag gccaaaatcc ccctgcacag cattgctgat atagtgccag aactggtgat
      601 acagcggctg atccaacccc agctgctgac gcaccagttc tataacctgc gcatcagctt
      661 cgggcccggc aatcaatcgc gccggatcgc cgggcagcat atgcacaaat aaaaacacca
      721 gcaccgagac gataaacagc gtcggaatca accccagtaa gcgtttgata acgtaattaa
      781 gcattccact ccctgcgttg cttattgcaa atccgcgtct tcaaagctga agccggtgtc
      841 tggcatgatc caaaaaccgg tcaggttttt actgtgtgcc gacaccagtt tttctaccac
      901 cagcgggatc cacggcgact cttgccagat gatatcctgc gccgccttat acaagcgggt
      961 cttttccgcc ggatcattag ttttcagtgc ctgagccagg aagtcgtcca cctgtttatt
     1021 gctgtaaaat gcggtattaa acagcgtcgg tggccagttt tgcgaggcga acagtggcga
     1081 tagcgcccag tccgcttcgc cggttgaagc cgaccagcca gtgtagaaca tccgcacgcc
     1141 gctctctttt tgccctttac cttcaacttc tgccgcccgc tgtccggcat ccatcgcagt
     1201 cacctggact ttaatcccga cctgcgctaa ctgctgctgg gtaaattgca gcactttctg
     1261 cgcggtgctg tggttatgtg acgaccacag cgtggtactg aaaccgttgg gatatcccgc
     1321 ctctttcagt aattcgcgcg ctttcactgg atcgtaaggc cacggtttat aactttgcgc
     1381 gtaggcgata cttggcggta ccacaccagt agctggcgtt gcatagcccg caaaggcaac
     1441 tttcaccagc gccggacggt taatggcgta attcagcgcc tcacggacct tcgggttatc
     1501 gaacggcttt tgcgtcacgt tcatactgat ataacgctgc ataattgacg gactggccat
     1561 caactcgata tttttgtttt tctccagcag tgtggcctgc tcgtagggaa tggggaaagc
     1621 aaactgcgct tcaccggttt gcagcattgc cgcgcgggtg ttgttatccg ccaccggacg
     1681 ccaggttatg ctgtccagtt tgggcaatcc tggctgccag taacccgcga attttttcac
     1741 cttcacaaaa tcggtctgat tccaggtatc cagttcatac ggtccggttc ccaccggatg
     1801 aaaaccaatc tccttgccat atttttccag cgctgccggt gaaatcatcg cggtcgccgg
     1861 atgggcaaga atattaataa acgctgagaa cggctgtttg agggtaatct ttactgttgt
     1921 tggatcgatc gcctccgttt tggcaatatt cttatacagg ttatagcgtt taagatgatt
     1981 cgccggatcg ctggcccggt ccagattcgc tttcaccgcc gcggcgttga aatcggtgcc
     2041 atcctggaat ttaatgcctt cccgcaattt cacggtgtaa gtaatgccgt catcggaaac
     2101 ggtataactc tccgccagca cgtttttcag tttcatctct ttatccagac cgaacagccc
     2161 ctggtaaaac gatttcgcta cggcctgaga taacgtgtca ttcgcgtcat acggatcgag
     2221 cgtggtgaaa ttcgatccta ccgccaccac cacatctttg gcagcgaatg cacaagatgc
     2281 catcaacgct gtcgcaatgc ccagcgccac taacccacta cggtgtacag ctcttgccat
     2341 tttattctcc gcctgaatgt tatctacgca tgaatgcgta ttctgattgt ggttgtgcga
     2401 cgtaatgccc cggcccgacg cattgcaacg agacggctgc cacctcttcg ccacgcagat
     2461 gaatattgct gggaagatcg tccgacagca gtacacgctg cggtcgttgt cgggacggtt
     2521 cagcgaccgg aactgccgcc agtaatttac gcgtataagg atgctgcggg ttttcgaaga
     2581 ccgcgcgccg tggaccaatt tcaacaattt gcccgagata catcaccgcc acacgatgac
     2641 taatccgctc taccacagcc atatcgtggg agataaacag atacgcaatg ccgaaatcac
     2701 gctggagatc gagcaacaag ttgataatct gcccgcgaat agaaacatcc agcgccgaaa
     2761 cggcttcgtc ggcaatgatc acttttggat tcaatgccaa cgcgcgagca atgcagatgc
     2821 gctggcgctg accgccggaa aactcatgcg ggtaacgcca ggcatgttca ggtaacaggc
     2881 ccacgcgctc cagcaaccac gcaacgcgtg cagccgcttc tttacctggc aataaaccgt
     2941 gtacacgcag cggttcgata atcgaatcac cgatggtctg acgtgggtcc agcgaagcgt
     3001 aagggtcctg aaaaataaac tgaatatccc ggcgcaatgc ctgaagtttg ccgggtgaca
     3061 aggtatcgat tcgctgaccg ttaaagataa tttcgccgcc ctgcgattcg accaggcgca
     3121 gcaacgcccg cccggtagtg gatttaccgc tgccagactc gcccaccagc gatagcgttt
     3181 cgccaggcca gagatcaaaa ctgactttct caacggcatg cacttcccgc gttacgcgat
     3241 tcaacaaacc gctgcgcaaa gggaaacggg tgaccagatt acgcacccgt aaaacaggtt
     3301 caccatccac caccgttttc tgctcgatgg ggggggcctg tttcgctgga tgttcaagcg
     3361 atatcaatgg gaaacgtcgg ggataatcta accctttcat cgcaccaagt tgcggaacag
     3421 cagctaacag cgcacgggtg taaggatgtt gcggtgcatg aaaaatctgt tcgacggtac
     3481 ccgtttccac cgcctcgccc tgatacatca ccagtacccg atcggcaatc tctgccacca
     3541 cgcccatatc gtgagtgata aagataacgc ccatcgacat ctctttttgc aatactttga
     3601 ttaattgcag gatctgcgcc tgaatagtga catccagcgc ggtggttggc tcatcggcaa
     3661 tcagcaccgc cgggcggcat gacagcgcca tcgcaatcat cactcgctgg cgcatcccgc
     3721 cagagagttg atgcggataa cgtgaaagaa tggtttgtgc ctcaggaatg cgtacctgat
     3781 ccagcatccg cttcgcctcg accatcgctt cttcacgact ggcgttctga tgcagacgaa
     3841 ttgattcggc aatctgttca cccacagtaa ataccgggtt cagcgatgtc atcggctcct
     3901 gaaatatcat cgccatatcc gcaccgcgaa catggcgcat ttgtgcagcg ctctgctcgc
     3961 taagttcaat cacttcgcga ctgcgccgct gcaacagcat tttatcgcac tgtactaaac
     4021 cgcccgcctg ttccaacagg cgcatcaatg ccaacgcagt cactgactta ccggagccgg
     4081 attcgccaac aattgccagc gtctcaccgc gttgcagact aaaagagaga ttgcggaccg
     4141 cagctatttt ctgctggtcc tgcataaagg caatattcag attttcaacc gccagcacat
     4201 taccggcatc aagttcatca ctgtgtggca acggtgtccc ctttttcacg gtagataccg
     4261 gtggttggcg tatcgcctgc gtagccccag gcgcgataca ttccttcggt gttaaacggt
     4321 agcgcgacat tcccttcatg gtcgatagcg attaagccac cgctaccgcc aagcgcaggg
     4381 agtttttcca ttactacccg ctcgcaggct tccgcgagac ttaatccgcc gtaatccatt
     4441 aacgcggcga tgtcatatgc cgccagcgcg cggatgaaga cttcgcccgt gccggtacaa
     4501 gaaaccgcca cactggcgtt attggcgtag catccggcac ccactaaggg actatcgcca
     4561 actcgtccgg gtaatttatt ggtcattccg cctgtggacg tggctgccgc caaattgccg
     4621 tctaaatcca acgccacggc ccccacggtg cccattttct gtttttcatc cagtggcgca
     4681 ccgctatggt cgaggacggt tgccccttcc ttgcgcgctg ccagtagttg ttcataacgc
     4741 aaaggcgtgg agaaaatctc cggcgagacg cgctccatgc cacgagcaaa cgcaaaattt
     4801 tctgcccctt cgccaatcat catcacatgc gggctttgct ccatcaccag ccgggcggca
     4861 agaaccggat tacgcagatg actaacgccc gccaccgcac cggctttcag ggtgttacca
     4921 tccatcacac aggcgtccag ttcatgggtt tcatcacgcg taaagacagc gccaattccg
     4981 gcgttaaaca gtggacactc ttccagcaga cgcaccgctt ccgtcaccac atccagcgca
     5041 ctttcgcccg cttccagcat tttctgcccg gtttcaacaa tggcagacaa cgcctcaatg
     5101 tagcgtaatt cctgttgcag actcatctgc gcgcggctaa ttgcacctgc gccaccatga
     5161 attgcaatga ctgctttgcc catagtattc gtccattaag ggtggctcgc cacttttgct
     5221 ataaatatca gaatttttat gaattactca ttcaattttt gaatatagaa agatggatat
     5281 ggcatgtaaa ggcaggagcc aacgctacgc ccgaggtggc atacagtctg tggcgttttt
     5341 ctgacataat aggcaaattc gattttgcct ccgcaggagt gttttcatgg aatttaccac
     5401 cggattgatg tcgctcgaca ccgcgcttaa tgagatgctt tctcgcgtca ccccactgac
     5461 cgcccaggaa acgctgccac tggtacagtg ttttggtcgt attctggcga gcgatgtcgt
     5521 ttcgccgctt gatgttccgg ggtttgataa ctccgcaatg gacggctacg cggtacgttt
     5581 agccgatatt gcctccaggc aaccgctgcc cgttgccggt aaatcctttg ccggtcagcc
     5641 gtaccatggt gaatggcctg cgggtacctg cattcgtatt atgaccggtg cgccggtgcc
     5701 ggaaggctgc gaagcggtgg tgatgcagga gcagactgaa caaacggaca atggcgtgcg
     5761 ttttactgct gaagtgcgta gcgggcaaaa tattcgccgt cgcggtgaag atatctctgc
     5821 aggtgcggtt gttttcccgg cgggaactcg cctgactacc gctgaactgc cagtgattgc
     5881 ttcactgggg attgccgaag ttccggtgat tcgtaaagtg cgtgtagcgc ttttttctac
     5941 cggtgatgaa ctccagttgc ccggtcagcc actgggcgat ggccaaatct acgataccaa
     6001 ccgtctcgcc gtacacctga tgttagaaca gttgggatgc gaggtaatca acttagggat
     6061 tatccgcgac gatccacatg ccctgcgcgc cgcatttatt gaagccgaca gccaggcgga
     6121 tgtggtgatc agttccggcg gtgtttcagt gggtgaagcg gattacacca aaacgattct
     6181 tgaagagctg ggggagatcg ccttctggaa gctggcgatt aaaccaggta aaccgttcgc
     6241 gttcggtaaa ctcagcaata gctggttctg cggcctgccg ggcaacccgg tttcagcgac
     6301 gctgaccttc tatcaactgg tacagccttt gctggcaaaa ctaagcggca acaccgccag
     6361 cggcctgccc gcgcgccagc gcgtacgcac agcgtcccgc ctgaaaaaaa cgccaggacg
     6421 tcttgatttc cagcgcggcg tgctgcaacg caacgccgac ggtgaacttg aagtgacgac
     6481 caccggacat cagggttcac atatatttag ctcctttagt ctcggcaact gctttatcgt
     6541 gctggaacgc gatcgcggca atgtggacgt gggcgaatgg gtggaagtag aaccgtttaa
     6601 cgcgttgttc ggaggcctgt aatggcggaa ctcagcgatc aggagatgct gcgctacaac
     6661 cggcaaatca ttctgcgcgg ttttgatttt gacggtcagg aggcgctaaa agattctcgt
     6721 gtgctggtag tcggcctcgg tggactcggc tgcgcagctt cgcagtatct ggcaagcgcc
     6781 ggtgtcggca acctgacgct gctcgacttc gacacggttt cgctctcgaa tctgcaacgc
     6841 cagacgctgc acagtgatgc tacggtcggg caaccgaag
//
LOCUS       DAMZET010000202         6680 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.202, whole genome shotgun sequence.
ACCESSION   DAMZET010000202 DAMZET010000000
VERSION     DAMZET010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.202"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..561)
                     /locus_tag="POB34_004205"
     CDS             complement(<1..561)
                     /locus_tag="POB34_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI5921057.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKGLERAYKNFFQKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVA"
     gene            569..1074
                     /gene="tnpA"
                     /locus_tag="POB34_004206"
                     /pseudo
     CDS             569..1074
                     /gene="tnpA"
                     /locus_tag="POB34_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1092..1760)
                     /locus_tag="POB34_004207"
     CDS             complement(1092..1760)
                     /locus_tag="POB34_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HDI5921058.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(2063..2656)
                     /gene="tehB"
                     /locus_tag="POB34_004208"
     CDS             complement(2063..2656)
                     /gene="tehB"
                     /locus_tag="POB34_004208"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HDI5921059.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIVNMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(2653..3645)
                     /gene="tehA"
                     /locus_tag="POB34_004209"
     CDS             complement(2653..3645)
                     /gene="tehA"
                     /locus_tag="POB34_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HDI5921060.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            3769..4749
                     /locus_tag="POB34_004210"
     CDS             3769..4749
                     /locus_tag="POB34_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HDI5921061.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(4744..5280)
                     /gene="rimL"
                     /locus_tag="POB34_004211"
     CDS             complement(4744..5280)
                     /gene="rimL"
                     /locus_tag="POB34_004211"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HDI5921062.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(5343..5567)
                     /locus_tag="POB34_004212"
     CDS             complement(5343..5567)
                     /locus_tag="POB34_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HDI5921063.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(5707..>6680)
                     /gene="opgD"
                     /locus_tag="POB34_004213"
                     /gene_synonym="mdoD"
     CDS             complement(5707..>6680)
                     /gene="opgD"
                     /locus_tag="POB34_004213"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HDI5921064.1"
                     /translation="FTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQL
                     GIAPMTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYT
                     DNNPKGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLD
                     NIVCFWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEH
                     YPEKWARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWY
                     PTSDSTDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
BASE COUNT         1779 a         1629 c         1535 g         1737 t
ORIGIN      
        1 agccacgcca gcatccagcc cgaccattga tgctgaaggg tgaaccggag ttgatacttc
       61 actttctgtc tgaatactga tgtaccactt accgcaggac tggctgacag tgacattttt
      121 cacaactccc gtgacctgac ggctgttccg gtagcgcatc cagcccagtt tcggcagaaa
      181 aatacggctg ttttcctgat cgagcttaac gccctgcggg tagcggaatg catcattctg
      241 tccccgcttt ttgaatcggg gaaaagcagc ccgcttctgg aagaagtttt tgtaagcccg
      301 ctcaaggcct ttcagtgact gttgcaatgg ctgtgagggc gaatctttaa gccattgcgt
      361 ttcagtggcg tttttccact caaccagcca ggaagccatt ttcccgtaag ggatgtattt
      421 attaccggcc tcatgattct cattctgaag cgccagagca cgattgaaaa cgaaacgaca
      481 tgcgcctgcg aagcgcctca tttcacgctc ctgttgacca ccgggtctta actggaattt
      541 aaatgcctgt aatcgtttca caggccaaat gatactttgg tctatgaata aagaaaccga
      601 tatccgacgg ggaagcattg tgttttcctg atgcatgttc acctggtctt tgtcaccaga
      661 taccgacgtc agatttttga ctatgacgcg acagaaaaac tacgcactta cttttcaaat
      721 gtatgtgctg attttgaagc tgaactggtt taaatggatg gcgaaccaga tcacgtccat
      781 ttgttaatta actatcctcc caaactggcg atatccagtc tggtaaacag cctcaaaggc
      841 gtatcgagta ggttactgcg acgagatcga ccagatattg cagtcaggta ttactacaaa
      901 ggcgttttgt ggagtcctgg ctattttgcc agtagctgcg gaggtgcgcc aatatccgtc
      961 atccgccaat acattgaaca acagcaaaca cctggtcagg tggaaaaccg cgccttatat
     1021 ccccgccctg aaggatgggg ttttacggcg caccggataa aaagcgccat ctaacgatgg
     1081 cgcgtctgga actacttttt gttaacgtca aacatggtgg catcggtcgc catgtcatca
     1141 ataacttgtt taatattttc gaaagccatt ggcgtacttt cgttattcag gtctttacct
     1201 tcgccctgac gaacgacttt gataaccggt ttattagtcg ctgcatcaat cagctcacct
     1261 tcaaaataga ggcgagtatc catggtacgg tggcctgtag ccatttgtgt ccccgcaacc
     1321 actaatgcaa caggaaccac ttcatagaat tgcagccctt ctttgctggt atctacaccg
     1381 gtaatggccc cacggaaaat cagactacgc ggcccagcgg tggtaaccag tggtttacgc
     1441 tgcgctatcg cttctttcat ttcggtgttg gtatagttca aaattttatc cagaactttc
     1501 tgccctacct gggtagaagg ttttggtacc ggataataag tgattgggtt ccagacgatg
     1561 ctgtcatatt tgctttgatc aaaactcggg tctacccaac gtaaaacagg tttacctgtt
     1621 gctgaggttg tttcttttaa atcagagtaa ttgtttaaaa aaccagaata tttatctggc
     1681 tgggtgattt tagaggcaca accagacaga gccaacaagc cgcataaagc tgcaactttc
     1741 gcaaatgatg tggtacgcat gaatagtttc ctgagatgat tatattactt tctaagttat
     1801 agcaaaaagc gccgcttcgt gttgtggcaa aaaaagtgtt ggtgaaaata aatttttcag
     1861 ccccttttca gacgacatag tcaatgccga aaaaggggct aagtcaatca tcagtagtta
     1921 aatttcagta agttaagctg ctaaaaaact tcgactactg tgattaattt cacatccctt
     1981 cgataaaaaa tatgcttacc gaaaagaaga acagtaaatc atctcagcgt ggggaatttt
     2041 cacgcgcctt attcttagcg ggtcattttt tacgtgccag catcgtggcg aaacgcagtt
     2101 taatacgatt accgttggcg tcggtgcggt gcagttcgcc gacgtcttca ttgtatttca
     2161 ccctctccca gccttcgtaa tatcgacgta attctccctc tttgaaggca aacgggaagc
     2221 cgacggtaca tggataatca gcggtatcca tcgccgccac aatcaggttg taaccaccag
     2281 gtttagtgca acgttgcata ttgacaatca gccctgggat ggttttagcc tcaaggaaca
     2341 tcagtaccac agtcgaaaga ataaaatcgt actgtctatc aaatgtgagg ttattcagat
     2401 caacgactcg ggtgtgtaaa ttatccagat tttcaatgga tttaatgcgc tcgacgttgg
     2461 cgatactcat ggcattttta tcccatgcgt caacatcata accattggct gccaggtaaa
     2521 gactgttacg accattgcca cagcccagat ccagcgtttt acccggttta accactttca
     2581 ccgcttccag tacttcagag tgtgtgcggg ttaattcata tttatcagta aaatagtttt
     2641 cgtcacgaat gatcattctt tgtcctctgc tttcattaaa acggcgcgct cggttctgac
     2701 taacaatttt ccctgcatca gaagcgcaaa agtacggatg agcagtattg caataataaa
     2761 attggtaaag ataaacagcg gcaccgccag cgtatggaaa aatccattat cgctgccact
     2821 ccccagatgc aaaccggtgg ttgccagtgc agatacgccg aacgagaaac tccagaatga
     2881 agcattaaat ggctgggaga gataccatgg catcagacgt agcataaaca gcagttgcag
     2941 cagtccataa ccgaaaagca ttttcgccag cgtgtcaccc tcgccgccgt tgacgctcag
     3001 ccaggcacta caagccacca gcgcaggagc gagctgaatg ccgagtgatg tccgcagtgc
     3061 cgtgggtaat tctcccgaac tgcgcagacg ctgcaagatc accggttcaa ggcttagcca
     3121 tgagaaaacg cctgcgccta aaaacaccag accggcgtcg gtgtagccca acgcaccaca
     3181 ggccattgcg ctgataaagt tgttggcaac tgtcggcaga tacagtccag gcgtggtagc
     3241 ttcttcaggg tgagatccgc gccataatcc cgcagtttgc caggcggcat aagccaactg
     3301 aaccacgaca ccaaaactga acaggcacac cgccagtggg cgaaaccacg gaacaaaacc
     3361 aatcgccacc agcatcgtcg ttgccggaaa caaactcaca aaactgctca gcactggatg
     3421 gcgaacttcc gccagcacgc tatgcggaaa gcgtatgagt cgggtaataa atgcgctagt
     3481 taataatccc cagatgatca tcgccagaat caccagccca tcccctaacc agtggctgac
     3541 ctgccaaacc tggctggcat agcgccaggc aaatcccatc cctatcgtcc ccaacacaat
     3601 accaaagtag cctgccggca aattgagcac tttatcgctc tgcatcttct tactcatttt
     3661 gtttaattta taaagtatat ttgaaatgca ttttatggaa ttttctcgag catagccaga
     3721 gccgcagaat ttgctacggt tatagtaagt cacctgtggg aaaacgctat gcgcaaatat
     3781 cgtctaagtg aagaacagcg agcctttagt tatcaggaag atggcactaa aaaaaatgtg
     3841 ttattacggc agattatcgc cattagcgat tttaacgatg tgatagcagg aaccgctggc
     3901 ggctggatcg atcgcgaaac ggtgttagca caggaaggaa attgctggat ttacgaccag
     3961 aatgctattg cgttcggcgg aacggtgatt tccggtaata cgcgcatcac cggcaccagc
     4021 gtactgtggg gagaggttta cgcaacggat aatgtctgga tcgataatag tgaaatcagc
     4081 cagggtgcat acataagtga cagtgtcacc attcatgact cattagttta cgggcaatgt
     4141 agaatatttg gtcacgcctt aattgatcaa cattctatga tcgtcgccgc acaaggctta
     4201 acgcccgacc atcaactcct gctccaaatt tatgatcgcg caagggttag cgcctcgcgt
     4261 attgtccacc aggcacaaat ttatggcgat gccgtcgtcc ggtatgcttt tattgaacat
     4321 cgcgccgaag tttttgattt tgccagtgtt gaaggtaatg aagagaacaa cgtctggcta
     4381 tgcgattgcg ctaaagtcta cggtcacgcg caggtgaaag ctggcataga agaagatgcc
     4441 attccgacga ttcattacag ttcgcaggtg gcggaatatg ccattgtgga aggtaattgt
     4501 gtgttaaaac atcatgtctt gattggcgga aatgccgtgg tacgtggtgg accgattctg
     4561 ctcgacgagc acgttgtaat acaaggtgag agccgtattt caggtgcggt gataattgaa
     4621 aaccatgtgg agttaaccga ccacgccgtc gttgaagcct ttgatggcga cacggttcat
     4681 gtccgtggtc caaaagtcat taacggcgag gagcgcatta cgcgaacgcc gctggcaggg
     4741 ttattatgaa tcgataatac gcgcgtataa gttcacatca tcataggcat cattcaggaa
     4801 ctcagcctgt ttcaggcaac cttcaaggat aaaaccattg cgcaaagcga cctggttgct
     4861 ttgcggattg tccacccgac atttgatcac gaagcgtcta agttcaccag actgagcgta
     4921 atgatgaatc aatgcctgca gcgcctgaga aatgatcccc tgcccctgat gagattcgtc
     4981 cagccagtag ccgatttcag cagttttatt cagtggttca atacgattaa acgagataac
     5041 gccgataagt tcatcttctt tgaaaatcat gaacattttg gcatagccgc gttgatgcaa
     5101 catcacatta ccctgcaccg tttttcgcgt gtcctcttca ctctgaacga actgcggcca
     5161 gtttagcgac tgctgtaacc aggttttatt tttacagatt aactgataaa gaggtgtgac
     5221 gtgactttcc gcaacagcat gtaattcaag tgattcgctt acttttatcg tttcagtcat
     5281 cttatctcca gtcaatccat gctgttgtta aaaaagccgc ctggctggcg gcttaggaaa
     5341 gtttacacct ctttgacgct ctcctgctgc aggattttga gcatctcatc ttttaactgt
     5401 agcttttgtt ttttcatgcg gaccacttcc gcattgtacc ctcgaccgtc ggaaccttcc
     5461 tttctggcaa tttcatgatc aagtttattg tgtttatcga acaaggacat aaagcgagga
     5521 ttttcgtttt tcagacggga tattaaatct cggtattctg gaaacatacg cactccctgt
     5581 tagtggctga caaggtgtat ctgaaataca caccatcaga ttactcattt aacttaggga
     5641 aaaatgcgag cgggatcgtg tttaaccaga cggcacctcc cgaagaaggt gccgaagaaa
     5701 aaacgattaa ctcatcacgc ggtcgtcaac atactgacgt ttatccggcg ctggcgggaa
     5761 atactgatac agccatgttt cactgatagc gtcgccctga caacgcagat acatccgcat
     5821 atcgaccgga tcagtggagt ccgaagtcgg ataccagtca aactgaatac gataaccatc
     5881 aatgggttca atatagagaa tttcgatttg cttcgcttcc ccactggaaa gcgtaatcac
     5941 cggctcaatg ccttttggtg cggcagcttt cagatcacca ccaacgaaat cgacggcaaa
     6001 acgacgcgcc catttttcgg gatagtgttc acctggtgcc caaccttccg ggaaaccgcc
     6061 catgccggta cgcgtcgcca taacgcgcgc taatgggcaa tgaacaggcg gttgcgcact
     6121 ccagtacaga cgatactgga atgcaaactc atcacctgct tttacagctt tttctggctg
     6181 ccagaagcag acaatgttat ccagcgtttc gcccgttgtt gggatttcca tcaggccgat
     6241 ggtgccctta ccccacttgt tacgcggctc cacccacaga cttgggcgtt tgttatacca
     6301 gcccataatg tcctgataat gggagaagtc acgatccagt tgcaataaac caaacccttt
     6361 cgggttgttg tcggtgtaag cattgaactg caatttttgc ggattattca gcggacggca
     6421 aatccactcg ccgttgcccc gccacatgga cagacgatca gagtcatgaa tttgcggatg
     6481 aattgtatcg cacatccgac gttcattagt accgcagctg aacatactgg tcatcggcgc
     6541 aatgcccagc tgtttaatgt ctttgcgcgc atacaggtga ttttccacat ccataatcac
     6601 ctgacttttc tcacaatgga tagtgaactt ataggcacca gtaatgctgg cgctatcgag
     6661 caacgcataa acggtaaagg
//
LOCUS       DAMZET010000203         6670 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.203, whole genome shotgun sequence.
ACCESSION   DAMZET010000203 DAMZET010000000
VERSION     DAMZET010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.203"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(81..365)
                     /gene="gatB"
                     /locus_tag="POB34_004214"
     CDS             complement(81..365)
                     /gene="gatB"
                     /locus_tag="POB34_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HDI5921065.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(396..848)
                     /gene="gatA"
                     /locus_tag="POB34_004215"
     CDS             complement(396..848)
                     /gene="gatA"
                     /locus_tag="POB34_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HDI5921066.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVYDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(858..2120)
                     /gene="gatZ"
                     /locus_tag="POB34_004216"
     CDS             complement(858..2120)
                     /gene="gatZ"
                     /locus_tag="POB34_004216"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HDI5921067.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIESTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(2149..3003)
                     /gene="gatY"
                     /locus_tag="POB34_004217"
     CDS             complement(2149..3003)
                     /gene="gatY"
                     /locus_tag="POB34_004217"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDI5921068.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(3313..4365)
                     /gene="fbaB"
                     /locus_tag="POB34_004218"
     CDS             complement(3313..4365)
                     /gene="fbaB"
                     /locus_tag="POB34_004218"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HDI5921069.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            4622..5899
                     /locus_tag="POB34_004219"
     CDS             4622..5899
                     /locus_tag="POB34_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5921070.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYPEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            5896..>6670
                     /locus_tag="POB34_004220"
     CDS             5896..>6670
                     /locus_tag="POB34_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDI5921071.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIA"
BASE COUNT         1599 a         1681 c         1615 g         1775 t
ORIGIN      
        1 gaaaaaataa tgatgacaat cggaagcatc accgtagggc cgaggtcgag aatataacgc
       61 atgacttctg aaaacatagg tcacccctgt aagatagtca gaattttatt ttgtaatgct
      121 tcgataccga caccagaaat aaaaggcatg ccgtgaacta acggaatatc gccaaaacta
      181 cgatccactt tggcagtggt gcatatcaaa tgcacaccat ccatataggt ttctatttca
      241 ttaacccgac actggattaa ttcaacagga atattatgat tctgacacaa ctctttaatt
      301 tcttccgccg ccatcgtaga ggtcgcaacc gcgcctccgc aagcgacaat aatcttgcgt
      361 ttcataaata gtccttttta ttgttacaga gggatttatt cgtctgaatc taaaacatac
      421 tttgtgaagt attcctttaa ctgagtttca ggaagagtaa ttagtgtctc gacgatatcg
      481 ggctgttgta acttgccaaa taaacagcgt aaaagtttca attgctgctg cggattttcc
      541 acaatcaacg caataaccaa cgacacctcc acgtcgttat catcatccgc ttgctgaaaa
      601 tgaactttat ttgttggcct taacagataa atggctgacg acttagcatg aatcgcctca
      661 caatgcggta tagcaatggc gtgctgctca agcattatcc cggtagggaa ttctgcttct
      721 ctggcaatta atgcctgtgg ccaggtatca taaaccacac ctttcgcgag catctcatta
      781 ccgatatggg ttaaaacttc gctacgatcg acaaaagaaa ttccgctacg aacaaacagg
      841 ttagtcatat accgtcctta ttccgcacag ccgtagcgat aggcgcgcaa aacatcataa
      901 attttatcca taatcagctg atgcggtatt gctgataatt ccccggactg aatgcgttca
      961 aattgtttgg gaagatactg actaatcatg cccagtggga tgtccacacc ttcaagattc
     1021 accatcatcg tttcgacgct atttttaatc cgactatgcg gccagtaata acgaatacga
     1081 tccgacaggc tgtaacgaat atccagtaat gaatcgttaa aacccgtacg ataatatttt
     1141 ttccagtatt gcggttcgtc gagcatcact tcttcaatta ccgccaggca accgctgcga
     1201 ttttcagggg cgataagttc ctgctcaatt tgtgccagtg caaatatcgc ctcgcgtaaa
     1261 gcaaaggtta atgcgggacc gactttcaat attgcaaagt gatcgcggac taattcccaa
     1321 taagccgtcc gggtctggta atcggtagaa tgtgcttcat aaaccattcg ggtgctttct
     1381 atccattgcg ccagcggctg cgcttcctgc ggctgataat ggataatatt gctgtgatca
     1441 aattccacgc ccggctgcac cacgatggca atcacgcgtg ttaacgcctc tgtcagccca
     1501 cgggcaataa aggccttttg atgcgtacgt aaagtattgg cggcatcttc aacatgggtg
     1561 atgtgtactg actgaatggc gctggcctca ccgcccggaa ccggaacttc ggtgccaatg
     1621 acatagctca gttgctcacg ctggcaatct gtcgccacac tttccgcagc aaagcaaagc
     1681 acagcagctc gttccgcaac cgtttctggt gctaacggta tgggatcccc cgcgcaggac
     1741 attgacgcat caagatgaat tttactgaaa ccggcacgaa catattcctt taccagctcg
     1801 acggattttt ccatcgccgc atccgcattt tcttgctgcc agcagtttgg ccccagatga
     1861 tcgccgccga gaataatgcg ttcgcgtgca aacccaactt tatcggcaat cgtaaaaaca
     1921 aattcgcgaa agtctgccgg tgtcattccg gtataaccgc caaattgatt gacctggttt
     1981 gacgttgctt caatcagcac tttgcgcgtg ctgttgcgat caaatgccag cgccgcttcg
     2041 ataaccaacg gatgggcaga acagactgaa catatgccga tatgttcacc agctttatgc
     2101 cgggcaatta acgttttcat gttttttcct tattaaatgg caggtgcgtt atgccctgcc
     2161 ctcgcagcca caatcggcaa tcactttgct caccacatcg cgcattgcgg atttagccga
     2221 ctgcaaataa tcccggggat cggtcgcttc agggtgctcg gtcaggtaat tttttaacgc
     2281 ctgcgagaag gcatttttca gctccgttgc aacgttgatt ttgcatatcc ccagtttgat
     2341 ggtttgctga atatccttag tcgataaccc tgacgcgcca tgcagcacca gcggtaagtt
     2401 cacccactgg cgaatgttct ccagtcgaga aaaatcaagc gccggtgcgc tggcatacat
     2461 cccatgagcc gtgccgatgg cgaccgccag ggaatcaatt ccggttgcct cggcaaattc
     2521 acgcgcctga gcggggttgg tataaaaagc atcggcttca ttgacttgca catcatcttc
     2581 ctggccgcca agttgcccca gctccgcttc gacgctgaca tcaaagcgat ggcaaaaatc
     2641 caccacctct ttgacccgtg aaatattttg cgcaaaaggc aaatgcgagg cgtcaatcat
     2701 gactgagcgc acgccagaac gaaccttctg agcgatatcg tcaaatttcg tgtgatggtc
     2761 gagatgaatt gccagtggat ggtgatattg cttcgccatc gcgctgacca gcgccaacag
     2821 attttctgta ccagcatgag taaatgtgcc aggcgttccg gcgatgatga ccggcgcatg
     2881 caggttggca gcggtttcca ccaccacttg catcgtttcg agattgtgaa tattgaatgc
     2941 cggaaccgca taaccgccgc gctgtgcgtt gttcagcatc tgctttgtcg ataccacgta
     3001 cattttcata tcctgtcgtt tgttttcgat ttcaaaatat aatgaaatga tttgttttta
     3061 aatgtcgaga taacgatcac aaaaacgaca atgtgaaaat tgttcgagga gtaaaaggca
     3121 aaaaaaaacg gcctcccgat agggaagccg tagcaaagtg cgcgtgtttt tatgccggat
     3181 gcggtgtaaa catacgtggc agtgctatgt aggcatgata agacgcttcc gcgtcgcatc
     3241 aggcataggt tgccggatgc agtgtaaacg ccttatccgt cctacggggt ggtgctgttc
     3301 gtatgaggcg tatcaggcaa tagtaatttt gctatcgaga taaacgtcct gcacggcatt
     3361 aatcagtttc acgccgtcag ccatcgattt cttgaacgcc ttacgtccaa gaatcagccc
     3421 cattccgcca gcgcgtttgt tgataaccgc agtacgcact gcatcgctga ggtcagtttc
     3481 accgcccgca gcaccgccgg agtttatcaa cccagcccga cccatatagc agttagctaa
     3541 ctgataacgc accagatcaa tcgggttttc gctggtcaat ttgctgtaaa cacgatcgtc
     3601 ggtgtaaccg taattaattg ctttatagcc gccgttattt tccgccattt tttgtttgac
     3661 gatatctgca ccgatggttg ccgccagatg gtttgcctga ccggtcaggt cggcggaaac
     3721 atggtaatca acgccatctt tcttaaaggc ggagttacgc aaataggccc acagcactgt
     3781 caccataccc agctcgtgcg cacgttcaaa agccgcagaa atttcttcaa tctggcgacg
     3841 tgactcttcc gagccaaaat agatagtcgc accaaccgca accgcgccca tgttgaacgc
     3901 ctgctccacg ctggcataca gcgtttgatc gtaggtattc gggtaactta gcgtctcgtt
     3961 gtgattaagt ttgacgagga atggaatgcg atgcgcataa cgccgcgata ccgacgccag
     4021 cacgccgtaa gttgacgcca cacagttaca gcccgcttcg atcgccagtt caacaatgtt
     4081 tttcgggtca aagtagagcg ggttagcagc aaatgaagct ccggcagagt gctcaacgcc
     4141 ctggtcaacc ggcagaatag aaagatatcc tgtgccagcc agacgcccgg tgttgtacaa
     4201 cgtctgcata ttacgtaaca ccgctggcgg gcgattattg tcaatcatta cgcggtctac
     4261 gtagtcatgt ccggggagat aaagctggtc agaaggaatt gtcatacaac ggtgctgtaa
     4321 aaggttgtcg gcgtctttgc caagcaactg cgcaatatct gtcattacta tgctcccgta
     4381 aattccgatt ggatatcggc tatggattgt cctggcccgc cttttgcggg caatcataat
     4441 cctggtcgtt acgggcgagt ttttccatct tttggatgtt ttttcagcgt tttctgttgg
     4501 ctcgattcat cagaaaaaat gttagcgcgg tcaagttttc accgcaaagg tatttaaaag
     4561 gtattattaa gtggtattgt catcgcgtac cttacattac ctgtcatgaa ggaattaaaa
     4621 gatgaaaaca acagcaaagc tgtcgttcat gatgtttgtt gaatggttta tctggggcgc
     4681 gtggtttgtg ccattgtggt tgtggttaag taaaagcggt tttagtgccg gagaaattgg
     4741 ctggtcgtat gcctgcacag ccattgcggc gatcctgtcg ccaattctgg ttggctccat
     4801 cactgaccgc tttttctcgg cgcaaaaagt gctggcggta ttgatgttcg caggcgcgct
     4861 gctgatgtat ttcgctgcgc aacagaccac ttttgccggg ttcttcccgt tactgctggc
     4921 ctactcgcta acctatatgc cgaccattgc gctgactaac agtatcgctt ttgccaacgt
     4981 gccggatgtg gagcgtgatt tcccgcgcat tcgtgtgatg ggcactatcg gctggattgc
     5041 ctctggtctg gcatgtggtt tcttgccgca aatgctgggg tatgccgata tctcaccgac
     5101 taacatcccg ctgctgatta ccgccggaag ttctgctctg ctcggtgtgt ttgcgttttt
     5161 cctgcccgac acgccgccaa aaagcaccgg caaaatggac attaaagtca tgctcggcct
     5221 ggatgcgctg atcctgctgc gcgataaaaa cttcctcgtc tttttcttct gttcattcct
     5281 gtttgcgatg ccactggcgt tctattacat ctttgccaac ggttatctga ccgaagttgg
     5341 catgaaaaac gccaccggct ggatgacgct cggccagttc tctgaaatct tctttatgct
     5401 ggcattgccg tttttcacta aacgctttgg tatcaaaaag gtattattgc ttggtctggt
     5461 caccgctgcg atccgctatg gcttctttat ttacggtagt gcggatgaat atttcaccta
     5521 cgcgttactg ttcctcggta ttttgcttca cggcgtaagt tacgattttt actacgttac
     5581 cgcttacatc tatgtcgata aaaaagcccc cgtgcatatg cgtaccgccg cgcaggggct
     5641 gatcacgctc tgctgccagg gcttcggcag tttgctcggc tatcgtcttg gcggtgtgat
     5701 gatggaaaag atgttcgctt atccggaacc ggtaaacggg ctgactttca actggtccgg
     5761 gatgtggact ttcggcgcgg tgatgattgc cattatcgcc gtgctgttca tgattttttt
     5821 ccgcgaatcc gacaacgaaa ttacggctat caaggtcgat gatcgcgata ttgcgttgac
     5881 acaaggggaa gttaaatgaa aacagaacgt attctcggtg ctctttatgg gcaggcgtta
     5941 ggggatgcga tggggatgcc ctccgagctt tggccacgca gccgcgttaa agcacacttt
     6001 ggctggattg accgttttct gcctggacca aaggagaata acgcggcctg ttattttaac
     6061 cgcgccgaat tcaccgacga tacctcgatg gcgctgtgtc tggcggatgc gttactggaa
     6121 cgtgaaggca agatcgatcc ggatctgatt gggcgtaata ttctcgactg ggcgctgcgt
     6181 ttcgacgcct ttaacaaaaa cgtactaggt ccgacctcga agattgcgct taacgccatt
     6241 cgcgacggta aacccgttgc tgaactggaa aacaacggcg tgaccaacgg cgcagcgatg
     6301 cgcgtctcgc cattaggttg tttgcttccg gcgcgtgatg ttgattcctt tattgatgat
     6361 gtggcgctgg cgtcgagccc gacccataaa tccgatctgg cagttgcagg tgcggtagtc
     6421 atcgcatggg cgatttctcg tgccattgac ggagaaagct ggtcagcgat tgttgattcc
     6481 ctgccttcaa ttgcgcgaca tgcacagcaa aaacgcatca ctaccttcag cgcctcactg
     6541 gcagcacgtc tggagattgc gctgaaaatt gtgcgcaatg ccgacggcac cgaatccgcc
     6601 agcgaacagc tttaccaggt cgttggcgca ggtaccagca ctattgagtc cgttccgtgc
     6661 gccattgcgc
//
LOCUS       DAMZET010000204         6630 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.204, whole genome shotgun sequence.
ACCESSION   DAMZET010000204 DAMZET010000000
VERSION     DAMZET010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.204"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..976)
                     /gene="tgt"
                     /locus_tag="POB34_004221"
     CDS             complement(<1..976)
                     /gene="tgt"
                     /locus_tag="POB34_004221"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDI5921072.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEIL"
     gene            complement(1032..2102)
                     /gene="queA"
                     /locus_tag="POB34_004222"
     CDS             complement(1032..2102)
                     /gene="queA"
                     /locus_tag="POB34_004222"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDI5921073.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            2195..2776
                     /gene="acpH"
                     /locus_tag="POB34_004223"
     CDS             2195..2776
                     /gene="acpH"
                     /locus_tag="POB34_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HDI5921074.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(2781..4598)
                     /gene="malZ"
                     /locus_tag="POB34_004224"
     CDS             complement(2781..4598)
                     /gene="malZ"
                     /locus_tag="POB34_004224"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HDI5921075.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIVLRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(4754..6127)
                     /gene="proY"
                     /locus_tag="POB34_004225"
     CDS             complement(4754..6127)
                     /gene="proY"
                     /locus_tag="POB34_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HDI5921076.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFEH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(6203..>6630)
                     /locus_tag="POB34_004226"
     CDS             complement(6203..>6630)
                     /locus_tag="POB34_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDI5921077.1"
                     /translation="TCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLSQLIQISVP
                     VLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAFSDILPSWA
                     QRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
BASE COUNT         1653 a         1873 c         1644 g         1460 t
ORIGIN      
        1 ctaatatttc gttgcaacgg tcaagatgat gcaagtaagc gcgtgaataa ttgcgacagg
       61 tgtagcaatc acactcagga tcgagtgggc cagtatcgct cttatacttc gcattgcgga
      121 ttttcaccac gccatcggtc acgaacaaat gaccatttcg ggcgttgcgg gttggcatta
      181 cgcagtcaaa catatcgata ccacgacgta cgccttcaac caggtcttct ggtttaccaa
      241 cgcccatcag gtaacgcggt ttgtctgccg gaatttgcgg gcatacatgc tccagaatgc
      301 ggtgcatatc tgctttcggc tcacccacag ccagaccgcc gacagcgtag ccatcaaaac
      361 cgatatctac cagaccttta acagaaatat cacgtaaatc ttcgtaaacg ctgccctgaa
      421 tgataccaaa cagcgcattt ttgtttccga gactgtcaaa acgctcacgg ctacgcttcg
      481 cccaacgcag agacatctcc atggagcgtt ttgcgtaatc ccagtcagca ggatacggcg
      541 tacactcatc aaagatcatg acgatatccg aaccaagatc gtactgaatc tccattgatt
      601 tttcaggatc gaggaaaatc ggatcgccgt tgatcgggtt acggaagtgc acgccctgtt
      661 cggtgatttt acgaatatcg ccaaggctga agacctggaa gccgccggag tcggtgagga
      721 tcggcccctt ccactgcata aaatcgtgca gatcgccgtg cagtttcatg atttcctggc
      781 ccgggcgcag ccacaggtgg aaggtgttgc cgaggataat ttgcgcgcca gtggcttcaa
      841 cttcttccgg cgtcatccct tttacggtgc cgtaggtgcc aacaggcata aaacaaggcg
      901 tttccactac gccacgatca aagaccaggc ggccacggcg tgcgcgaccg tcggtggtgt
      961 ccagttcaaa tttcattttt tctccactac gtcagaaaaa cagtccaacg ttttaaacca
     1021 gcgccgcgga attactcccc gacgcgctca ttaattgcct gcggattgta cgtgataaac
     1081 atcgcatcac cgtaactaaa aaagcgatat ttctcttcta ccgctgcttt ataggcattc
     1141 atggtgtgtt gataaccggc aaaggccgaa accagcataa tcagcgtcga ttccggcagg
     1201 tggaagtttg tcaccagcgc atcgaccact ttgtactgga agcccggata gataaagatt
     1261 tgggtatcgt caaagaacgg ttcgataaga tcgtttttcg ccgcctgagc cgcgctttcc
     1321 agcgaacgta ctgaggtggt gccaaccgca atcacccggt taccgcgcgc tttcgccgcc
     1381 agtaccgcgt ctaccacatc ctgcggcact tcagcgtatt ccgagtgcat gatgtgatct
     1441 tcaatggtgt cgacgcgcac cggctggaag gtgcccgcac caacgtgcaa cgtcacaaac
     1501 gccatctcca cgcctttggc gcgtaatttt tccagcaaag gttcgtcaaa atgcagacct
     1561 gcggtcgggg ctgcaaccgc gcccggtttt tcgctataaa cggtttgata aagttcgcgg
     1621 tcagcgtctt cgtccggacg gtcgatatac ggcggcagcg gcatatggcc gatgctgttg
     1681 agaatatcca gcaccgagcg ttcatcatta aattcgactt caaacagtgc gccgtggcgc
     1741 gcggtcattg ttgcgttaat actttcgtca tcgcccagca gcagttctgc gccaggtttt
     1801 ggcgctttcg aggcgcgaat atgcgcaaga atgcgtttgt cgtcgagcat ccgttcaacc
     1861 agcacttcaa tcttgccgcc gctggcttta cgcccaaaca ggcgcgccgg gatcacgcgg
     1921 gtattattaa aaaccagaag atcgccgggg ttgagcttat caagtaaatc ggtgaaagta
     1981 ccgtgcgtca gcgcgcccgt cggcccgtcc agcgacagta aacgacagct actgcgttca
     2041 ggcatgggat agtgggcaat cagggattcg ggcaattcaa aggagaaatc ggtaacgcgc
     2101 atgacactga ctcagactaa aataagaggc gggtagtcta gtgccggggc gctctccctg
     2161 caacctttac ccccttttgg atagaatata atcgatgaat tttttagctc acctgcattt
     2221 agcccatctc gcggaaagct cgctttccgg caatttactg gctgatttcg tacgcggaaa
     2281 tcctgaagaa agttttccgc ccgacgtcgt ggctggcatt catatgcatc gacgtatcga
     2341 cgtattaacc gacaatctgc cggaagtccg cgaagcacgg gaatggtttc gtagtgaaac
     2401 tcgccgcgtt gcgcctatta cgctggatgt catgtgggat cactttcttt cccgccactg
     2461 gtcgcagctg tcgccggact ttccgctaca ggaatttgtc tgttatgccc gcgagcaagt
     2521 gatgacgatt ttgccggact caccgccacg ttttatcaat ctgaacaatt acttgtggtc
     2581 agagcagtgg ctggtgcgct atcgcgatat ggatttcatc cagaacgtgt taaacggcat
     2641 ggcaagccgc cgcccacgtc tggatgccct gcgtgactcc tggtacgatt tagacgctca
     2701 ttatgacgcc ctcgaaaccc gcttctggca gttttatccg cggatgatgg cgcaggcgtc
     2761 acacaaggcg ttataaaacg ttagttcatc cataccgtag ccgaaatggc aggcaaagcc
     2821 agaatcccgt cagtcagttg cccatgccct tctttgcatt gccattgcac ggcattgagc
     2881 aacggtgacg cgggtagcac gacttcacaa gcttcgccac ggttgattgc caccagtaca
     2941 cgctgctgat tcagcacgcg gacaaatacc accacgttat cttccgcata cagcacctga
     3001 cagccgccat gacgtagcgc ctgacttttc ttacgcagca caatcattcg ctggtacagc
     3061 gcgaataacg ccgtatcctg cttttccacc tgccaaggga acggtttacg gcaaaacgga
     3121 tcgtttttgc catccagtcc tacttcatca ccgtaataaa tgcacggtac accaggccag
     3181 gtgaacagcc agaccaccgc cagcggcagg cgcgcaatat cccgaccgag cagcgtttta
     3241 aatcgcgcag tatcgtggct gtcgagctga ttaaacatac gtaattgttg ttgatgagaa
     3301 agccctgcgc ggtaattatc catccaggcc atacaggttt gggcatcaat ttgctgcgga
     3361 tcgtaagaga tatcggtatt ggcaagaaat ccccacaacg ggaatgtgaa gccacgatag
     3421 ttcatggcgg catcttccac atcggcctgt aaccattgcc gtgcatcgcc aaaatgttcg
     3481 ccgacaatat acgcttccgg ctgggtttct ttcgccgctt cggtgatccc agcaacgtgc
     3541 tgcatattat tgcgcgcccc acccgcctcc cccagcatat gcaccacatc cagccgccag
     3601 ccgtccatac tccacggcgc tttcagccaa tggcggacaa tactgtcttc cccgcgataa
     3661 atttcattca ccagactttc cgactgataa tccagcttcg gcaagctggc atagccaagc
     3721 cagtcgagcg ccgtgccatc atcactaaac gagtaccagt cgcgccaggg cgattcaggg
     3781 ttgtgacaag ctccccccgt gccacgattg tgcctgtcaa accaggcatg ggaatcgcca
     3841 ctgtggttaa acacgccgtc cagcaccagc cgcattccca gctgctgcgt attgtgtcgc
     3901 aaacgcagca acgccccatc accgccaaac tgcggatcga catggcgata atcctcggta
     3961 tcgtatttat gtacgctggg agctttaaac accggattga gatacagcgc cgtcacgcca
     4021 agctttttca gatacggcag tttttcgctt atcccgtcca gatcgccgcc atagaacgtt
     4081 gatccgcccg cctgcgccgt gaccggttca tcccagtcac gcaagatgat ctcttgtccg
     4141 gctgcatgat ggtaatagac atgatcctgt tcagcttcac gaggaagact acgcgcaaaa
     4201 cgatcaggga agatctgata aaaaatctga tccgcagccc attgtgggcc gatatccggt
     4261 acatcgacgg caaactgctc cagtcgtgcc ggcggcattc ggctgaagcc ctgcggtgta
     4321 aaccaacgct ggcgatcgtg ccacagcagt ttgaaactgt aacgccgccg gggttgtccg
     4381 ctggagagat caatcgccgc acgccatgcg gtgacgccag gctgctgctg actgcgctgc
     4441 ttatgcatcg atactgacat ttcttcgtta tcgtgttctg tacgcagcat aatgcgctgc
     4501 ggtgggtctt cgcccgtcag ccacagtgta atgagcagtt gatctttgct ttgtttaaca
     4561 aatgggggca ccggcaggtg ccatgcattt aacatcataa ttcccctgtg atgaaaatct
     4621 gatcaccttg ccatagccta atgcaggatc actcatcatc tgaaggatat ttgggggagg
     4681 aggacggggg cggataactt tcggaatagc cggatcagga tttcctcctg aaccggctta
     4741 cagaacgcaa gtattattgg ttttcagcca gctgacgatc gtggcggcgt ttaaacatcc
     4801 agccaatcaa cagcacaaca atccacgcga aaccgacata cagcgagata cgcgtatccg
     4861 ggtgataacc aatcaacccg ataataaaga gcaggaaaat cagcccgccg atggtcgttg
     4921 ctaccccacc cggcacttta aatttcagcg ccttaacttc ttctggcggc aaacggcgac
     4981 ggaaggcaat ttgcgacagc aggatcataa tccacaccca caccgtggcg aaggttgcca
     5041 gcgaagcgat caccaggaag acgttttccg gcatgatgta gttcagatac accgcaaaca
     5101 gcagcgcggt agtcatcacc agcaccgtaa cccacggaat accgcgacgc gacgttttgc
     5161 tgaaaatttt cggcgcgctg ccctgctctg ccataccgtg gagcatacgg cctacgccaa
     5221 atacatcact gttaattgcc gacagcgaag cagtcagcac aacaaagtta agaatgctgg
     5281 cggcaaaggt aatgcccata tgctcgaacg tcagcacgaa cgggctaccg gcagtgccaa
     5341 cctgattcca cgggtagata gacataatga cgaacagcgt accgacgtag aacaccagaa
     5401 tacgcatcgg cacggagtta atcgcacgcg gtatcgattt ctcaggatct ttcgcttcac
     5461 cggcggtaat cccgataatt tcgatcccac cgtaagcaaa catcaccatt tgcaacgaca
     5521 ttaccatgcc aagccagccg ttactgaaga agccgccgtt gctccacagg ttatgaatac
     5581 cggtcggttg cccgccgttg ccaatccccc agatgatgat gccgaaaccg gcgacaatca
     5641 tgatgatgat ggtggcgact ttaaagaacg agaaccagaa ttccagctca ccgaagacct
     5701 tcacgctcat caggtttacg gcgcaaatga tcagcaccac gctcagtacc caaatccagt
     5761 gcggcaccgt cgggaaccag acacccatat agataccaaa agcggtcaca tcggcgatgg
     5821 cgacaataag gatttcaaag cagtaggtcc atccggtaat gtaacctgcc agcgggccga
     5881 ggttttcctg cgcataacgc gagaaagagc tggcggccgg gttatgtacc gacatttccc
     5941 ccagcgcacg cataatgata tacgccgcga taccaccgat aatataggcc aacaacacgc
     6001 tcggaccggc cattttgatg gcgtctgccg aaccgtaaaa cagcccggtg ccaattgctg
     6061 aacccagtgc cataaagcgt atgtggcggg tacttagccc acgctttagc ttgttcttac
     6121 tttccatcaa ttcctaaccc atcaacacaa taaaaaacca cggggcatcg cagccccgtg
     6181 gttaaaacaa atgttcagtg atttagtgag cgctggaggt cacctgacga cctgccgcgc
     6241 gatcccagat aatggccaga accaccatca ccactgttgg cattaaccac gccagacctt
     6301 gttcggccag cggtaaacgc tgcgcccagg acggtaagat atcgctgaat gcagatgctt
     6361 tgatcccgtc gagaatacca aaaagcaggc tgataaacat cggcggagca atcacgcggg
     6421 acgaattatg ccaccatgag cgtgtaaaac ttaatacaac cagtgcgata cacggcggat
     6481 aaatagcggt cagcaccggt acggagatct ggatcagctg gcttaagccg aggttagaaa
     6541 ccaccatcga gaagccgccg aggataaaca ccagcgtacg ataagagagc ggtacgtact
     6601 gggcaaagaa ttctgcacaa gcacaggtca
//
LOCUS       DAMZET010000205         6415 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.205, whole genome shotgun sequence.
ACCESSION   DAMZET010000205 DAMZET010000000
VERSION     DAMZET010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.205"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..862
                     /gene="cysB"
                     /locus_tag="POB34_004227"
     CDS             <1..862
                     /gene="cysB"
                     /locus_tag="POB34_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HDI5921078.1"
                     /translation="MLEDELGIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKS
                     VAGEHTWPDKGSLYIATTHTQARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSK
                     GNADFAIATEALHLYEDLVMLPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYT
                     FGFTGRSELDTAFNRAGLTPRIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDL
                     VRVDAHDIFSHSTTKIGFRRSTFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEV
                     MFKDIKLPEK"
     gene            1157..1321
                     /locus_tag="POB34_004228"
     CDS             1157..1321
                     /locus_tag="POB34_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HDI5921079.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            1324..1491
                     /locus_tag="POB34_004229"
     CDS             1324..1491
                     /locus_tag="POB34_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921080.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            1605..1700
                     /locus_tag="POB34_004230"
     CDS             1605..1700
                     /locus_tag="POB34_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921081.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            1864..4539
                     /gene="acnA"
                     /locus_tag="POB34_004231"
     CDS             1864..4539
                     /gene="acnA"
                     /locus_tag="POB34_004231"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HDI5921082.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLAAEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(4603..5193)
                     /gene="ribA"
                     /locus_tag="POB34_004232"
     CDS             complement(4603..5193)
                     /gene="ribA"
                     /locus_tag="POB34_004232"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HDI5921083.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            5363..6127
                     /gene="pgpB"
                     /locus_tag="POB34_004233"
     CDS             5363..6127
                     /gene="pgpB"
                     /locus_tag="POB34_004233"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HDI5921084.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            6276..>6415
                     /gene="lapA"
                     /locus_tag="POB34_004234"
     CDS             6276..>6415
                     /gene="lapA"
                     /locus_tag="POB34_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HDI5921085.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAV"
BASE COUNT         1585 a         1507 c         1659 g         1664 t
ORIGIN      
        1 aatgctggaa gacgagctag gcattcaaat tttttcccga agcggcaagc acctgacgca
       61 ggtaacgcca gcaggacaag aaataattcg tatcgctcgc gaagtcctgt cgaaagtcga
      121 tgccataaaa tcggttgccg gtgagcacac ctggccggat aaaggctcgc tgtatatcgc
      181 caccacgcat acccaggcac gctacgcgtt accaaacgtt atcaaaggct ttattgagcg
      241 ttatccccgc gtttctttgc atatgcacca gggctcgccg acacaaattg ctgatgccgt
      301 ctctaaaggc aatgctgatt tcgctatcgc cacagaagcg ctgcatctgt atgaagattt
      361 agtgatgtta ccgtgctacc actggaatcg ggctattgta gtcactccgg atcacccgct
      421 ggcaggcaaa aaagccatta ccattgaaga actggcgcaa tatccgttgg tgacatatac
      481 cttcggcttt accggacgtt cagaactgga tactgccttt aaccgcgcag ggttaacgcc
      541 gcgtatcgtc ttcacggcaa cggatgctga cgtcattaaa acttacgtcc ggttagggct
      601 gggggtaggg gtcattgcca gcatggcggt ggatccggtc gccgatcccg accttgtgcg
      661 tgttgatgct cacgatatct tcagccacag tacaaccaaa attggttttc gccgtagtac
      721 tttcttgcgc agttatatgt atgatttcat tcagcgtttt gcaccgcatt taacgcgtga
      781 tgtcgttgat gcggctgtcg cattgcgctc taatgaagaa attgaggtca tgtttaaaga
      841 tataaaactg ccggaaaaat aatttcttgc gttattttcg gcacctttta tgtagcgaag
      901 gtgccggaat atattctctt ttgttactta tttctatacc gtgcacaata tttttatccc
      961 tcatatttat agggtaaatt acctgtaaag tagtgtctaa ttacccgcct cgcaaactcc
     1021 agttttcaaa tatctatttc aggtcaaaag attgaattat tcactggaga cgattcgtaa
     1081 attcgctgga tttttgacta aagtttcttt aggatttgtc tcatcgtatg attaattaca
     1141 ccaatcgttc aacgtcttgc tgataagtga tggcgattat atgaggttag caatgccttc
     1201 tggaaatcag gaaccccgcc gcgatcctga attaaaacgt aaagcctggc tggcggtttt
     1261 tcttggttct gcacttttct gggtggttgt cgcactgctg atttggaaag tgtggggata
     1321 actatggtcg gtcaggagca actggagtcg tcaccattat gccagcatag tgacaatgaa
     1381 acagaaacga aacgggaatg ttccgtcgtt attccagacg actggcaact aacatcgcag
     1441 cagcaagcct ttatagaact gtttgctgaa gatgatcagc cgaaacaata attatcatca
     1501 ttcttattac ccatttttaa tgaattaaag ggcttttaat acaccgcagc aataacagct
     1561 tgagttatct caacacaaaa taataaccgt taagggtgta gcctatgatc aacacaaata
     1621 tgaaatattg gtcctggatg ggcgcgtttt ctctgtcgat gctcttctgg gccgaactcc
     1681 tctggatcat tactcactga tccttgaccc cgctgcggcg gggttgtcat ttgctttgcc
     1741 acaaggtttc tcctctttta tcaatttggg ttgttatcaa atcgttacgc gatgtttgtg
     1801 ttatctttaa tattcaccct gaagagaatc agggcttcgc aaccctgtca ttaaggagga
     1861 gctatgtcgt caaccctacg agaagccagt aaggacacgt tgcaggccaa agataaaact
     1921 taccactact acagcctgcc gcttgctgct aaatcactgg gcgatatcac ccgtctgccc
     1981 aagtcactca aagttttgct cgaaaacctg ctgcgctggc aggatggtaa ctcggttacc
     2041 gaagaggata tccacgcgct ggcaggatgg ctgaaaaatg cccatgctga ccgtgaaatt
     2101 gcctaccgcc cggcaagggt gctgatgcag gactttaccg gcgtacctgc cgttgttgat
     2161 ctggcggcaa tgcgcgaagc ggttaaacgc ctcggcggcg atactgcaaa ggttaacccg
     2221 ctctcaccgg tcgacctggt cattgaccac tcggtgaccg tcgatcgttt tggtgatgat
     2281 gaggcatttg aagaaaacgt acgcctggaa atggagcgca accacgaacg ttatgtgttc
     2341 ctgaaatggg gaaagcaagc gttcagtcgg tttagcgtcg tgccgccagg cacaggcatt
     2401 tgccatcagg ttaacctcga atatctcggc aaagcagtgt ggagtgaatt gcaggacggt
     2461 gaatggattg cttatccgga tacactcgtt ggtactgact cgcacaccac tatgatcaac
     2521 ggccttggcg tgctggggtg gggcgtgggt gggatcgaag cagaagccgc aatgttaggc
     2581 cagccggttt ccatgcttat cccggacgta gtgggcttta aacttaccgg taaattacgt
     2641 gaaggtatta ccgccacaga cctggttctc actgttaccc aaatgctgcg caaacatggc
     2701 gtggtgggga aattcgtcga attttatggt gatggtctgg attcactacc gctggcggat
     2761 cgcgccacca ttgccaatat gtcgccagaa tatggtgcca cctgtggctt cttcccaatc
     2821 gatgctgtaa ccctcgatta catgcgttta agcgggcgca gcgaagatca ggttgagttg
     2881 gtcgaaaaat atgccaaagc gcagggcatg tggcgtaacc cgggcgatga accgattttt
     2941 accagtacgt tagaactgga tatgaatgac gttgaagcga gcctggcagg gccaaaacgc
     3001 ccacaggatc gcgttgcact gcccgatgta ccaaaagcat ttgccgccag taacgaactg
     3061 gaagtgaatg ccacgcataa agatcgccag ccggtcgatt atgttatgaa cggacatcag
     3121 tatcagttac ctgatggcgc tgtggtcatt gctgcgataa cctcgtgcac caacacctct
     3181 aacccaagtg tgctgatggc cgcaggcttg ctggcgaaaa aagccgtaac tctgggcctc
     3241 aagcggcaac cgtgggtcaa agcgtcgctg gcaccgggtt cgaaagtcgt ttctgattat
     3301 ctggcaaaag cgaaactgac accgtatctc gacgaactgg ggtttaacct tgtgggatac
     3361 ggttgtacca cctgtattgg taactctggg ccgctgcccg atcctatcga aacggcaatc
     3421 aaaaaaggcg atttaaccgt cggtgcggtg ctgtccggca accgtaactt tgaaggccgt
     3481 atccatccgc tggttaaaac taactggctg gcctcgccgc cgctggtggt tgcctatgcg
     3541 ctggcgggaa atatgaatat caacctggct gctgagccta ttggtcatga tcgtaaaggc
     3601 gacccggttt atctgaaaga tatctggcca tcggcacaag aaattgcccg tgcggtagaa
     3661 caagtctcca cagaaatgtt ccgcaaagag tacgcagaag tttttgaagg cacagcagag
     3721 tggaaggaaa ttaacgtcac acgatccgat acctacggtt ggcaggagga ctcaacctat
     3781 attcgcttat cgcctttctt tgatgaaatg caggcaacac cagcaccagt ggaagatatt
     3841 cacggtgcgc ggatcctcgc aatgctgggg gattcagtca ccactgacca tatctctccg
     3901 gcgggcagta ttaagcccga cagcccagcg ggtcgatatc tacaaggtcg gggtgttgag
     3961 cgaaaagact ttaactccta cggttcgcgg cgtggtaacc atgaagtgat gatgcgcggc
     4021 accttcgcca atattcgcat ccgtaatgaa atggtgcctg gcgttgaagg ggggatgacg
     4081 cggcatttac ctgacagcga cgtagtctct atttatgatg ctgcgatgcg ctataagcag
     4141 gaacaaacgc cgctggcggt gattgccggg aaagagtatg gatcaggctc aagccgtgac
     4201 tgggcggcaa aaggtcctcg tctgcttgga attcgtgtgg tgattgccga atcgtttgaa
     4261 cgaattcacc gttcgaattt aattggcatg ggcatcctgc cgctggaatt tccgcaaggc
     4321 gtaacgcgta aaacgttagg actaaccggg gaagagaaga ttgatattgg cgatctgcaa
     4381 aatctacaac ccggtgcgac ggttccggtg acgcttacgc gcgcggatgg tagccaggag
     4441 gtcgtaccct gccgttgtcg tatcgacacc gcgacggagt tgacctacta ccagaacgac
     4501 ggcattttgc attatgtcat tcgtaatatg ttgaagtaac aagtatttgc ttgccggtta
     4561 ttttgcttcc ggcaagcaaa ctaattacac aatgcaagag ggttatttgt tcagcaaatg
     4621 gcccattttc tcggctttgg tatcgagata atgttcgtta ttggggttac gacctacaat
     4681 caatggtacg cgttcaacaa tattaatccc tgcttcggtc agaatttcga cttttttcgg
     4741 gttattggtt aacaagcgga cttcattgac gccaaggagt ttgaacatat cagcgcaaag
     4801 agtgaagtcg cgctcatcag cggcgaagcc taactggtgg ttagcctcta cggtatcgta
     4861 accttgatcc tgcagtgcgt aagcgcggat tttattcagc agaccaatgt tacgaccttc
     4921 ctgacggtga tacagcaaaa taccacggcc ttcctcggca atttgcgtca atgccgcttc
     4981 gagctggaag ccacaatcgc agcgcaagct gaacagggcg tcaccggtca gacattcgga
     5041 atggacgcgc gcaagtaccg gggtatgccc ggaaatatcg ccatagacta gcgcgacatg
     5101 atcgtgtccg gttgccagtt cttcaaatcc caccatcagg aaatcgcccc atggggttgg
     5161 cagtttggct tctgccacac gtttaagctg catgaaattc tccagataat gctggttctg
     5221 tattggctta ttttgccata acgagaaggg gttcacctaa tcacgacgcg tcgatcgttc
     5281 acggaatggc acaaatctgt caatttttcc tggaactggc gttttcagtt atgattgtgg
     5341 gacttatcaa aaaggagagg ccatgcgttc gattgccaga cgtaccgcag tgggagctgc
     5401 actattgctt gtcatgccag tagccgtatg gatttctggc tggcgttggc aacctggaga
     5461 acaaagttgg ctactaaaag cggctttttg ggttactgaa actgtcaccc agccctgggg
     5521 cgtcattaca catttgattt tattcggctg gtttctctgg tgtctgcgtt ttcgcattaa
     5581 ggctgccttt gtattatttg ccattctggc ggccgcaatc cttgtgggac aaggcgttaa
     5641 atcctggatc aaagacaaag tccaggaacc acgacctttt gttatctggc tggaaaaaac
     5701 acatcatatt ccggttgatg agttctacac tttaaagcga gcagaacgcg gaaatttagt
     5761 gaaagaacag ttggctgaag agaaaaatat cccacaatat ttgcgttcac actggcagaa
     5821 agagacgggg tttgcctttc cttccggtca cacgatgttt gctgccagtt gggcactgct
     5881 ggccgttggt ttgctgtggc cgcgtcggcg aacgttaacc attgctatct tgctggtctg
     5941 ggcaacggga gtcatgggaa gccgcctgct gctcgggatg cattggccac gcgatctggt
     6001 agtagctacg ttgatttcgt gggcgctggt ggcggtggca acgtggcttg cgcaacgaat
     6061 ttgtgggcca ttaacaccac ctgcggaaga aaatcgcgaa atagcgcaac gagaacaaga
     6121 aagttaacgc tggttgattt tccgaattta gcccttaaat catcaacaat gcgcgtggat
     6181 gccatttcgc agacggcgcg aaaatggtac tttaaagggc tattgcggta agttgaccat
     6241 aatttattcg ctctaaccac ataacgggaa gtaatgtgaa atatttactc attttcttac
     6301 tggtgttagc gatcttcgtg atttcggtca cgttgggtgc gcagaacgat caacaggtga
     6361 cgtttaatta tctgttagcg caaggggagt accgtatttc cacattgctg gcggt
//
LOCUS       DAMZET010000206         6393 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.206, whole genome shotgun sequence.
ACCESSION   DAMZET010000206 DAMZET010000000
VERSION     DAMZET010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.206"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            112..525
                     /gene="msrB"
                     /locus_tag="POB34_004235"
     CDS             112..525
                     /gene="msrB"
                     /locus_tag="POB34_004235"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDI5921086.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            567..839
                     /locus_tag="POB34_004236"
     CDS             567..839
                     /locus_tag="POB34_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HDI5921087.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(1000..1641)
                     /gene="pncA"
                     /locus_tag="POB34_004237"
     CDS             complement(1000..1641)
                     /gene="pncA"
                     /locus_tag="POB34_004237"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HDI5921088.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(1652..2668)
                     /gene="ansA"
                     /locus_tag="POB34_004238"
     CDS             complement(1652..2668)
                     /gene="ansA"
                     /locus_tag="POB34_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDI5921089.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(2835..4691)
                     /gene="sppA"
                     /locus_tag="POB34_004239"
     CDS             complement(2835..4691)
                     /gene="sppA"
                     /locus_tag="POB34_004239"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDI5921090.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            4852..5403
                     /locus_tag="POB34_004240"
     CDS             4852..5403
                     /locus_tag="POB34_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HDI5921091.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            5520..>6393
                     /gene="selD"
                     /locus_tag="POB34_004241"
     CDS             5520..>6393
                     /gene="selD"
                     /locus_tag="POB34_004241"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDI5921092.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPRE"
BASE COUNT         1618 a         1595 c         1657 g         1523 t
ORIGIN      
        1 tgcagccatt tcgtcgagat caagtttagg acaaaatttt gccagttggt gttaatgttt
       61 tgttagaatc agtcacgtaa tgtgagcacg attaaagtga gatgtgagca aatggctaat
      121 aaaccttcgg cagaagaact gaaaaaaaat ttgtccgaga tgcagtttta cgtgacgcag
      181 aatcatggga cagaaccgcc atttacgggt cgtttactgc ataacaagcg tgacggcgta
      241 tatcactgtt tgatctgcga tgccccgctg tttcattccg aaaccaagta tgattccggc
      301 tgtggctggc ccagtttcta cgaaccggtg agtgaagaat ccattcgtta tatcaaagac
      361 ttgtcacatg gaatgcagcg catagaaatt cgttgcggta actgtgatgc ccatctgggg
      421 catgtctttc ccgacgggcc gcaaccaaca ggcgaacgtt attgtgttaa ctctgcttct
      481 ttacgcttca ctgatggcga aaacggcgaa gaaatcaacg gttgaaaaaa cgattcagca
      541 acttattcca caggagcgga aaaattatga atcttgatga cattatcaac agcatgacgc
      601 ctgaggtata ccagcgtttg tcgaccgccg ttgaactggg gaaatggcct gatggcgttg
      661 cgttgaccga ggaacaaaaa gagaattgcc tgcaactggt gatgctgtgg caagcccgcc
      721 ataataccga agcacagcat atgacaattg acacaaatgg tcagatggtc atgaagagca
      781 aacagcagtt aaaagaagat tttggtatct cagcaaagcc tattgctatg tttaagtgaa
      841 tgagttatct agtatttaag cctgttatta agggagggca aatatacttg ttaggacgtc
      901 gtgttttagc tgcaagtagg ttaaatgcaa cactgacaag tattatctgg cctacaatgt
      961 cattgaggaa atgtaggcca gagatgaatt caactaatat tacccctgcg tctcttccca
     1021 gtctgccagc gtatatagcg ttgccccagc tgctgacatc tccataaacg cgtgcgcact
     1081 gtcctggggc tggatattca cgccacgaca accatcggta atcacgttca ccttataacc
     1141 taactgtaac gcgtccagca cggtaaactt cacgcaatag tcagtagcca ggcccataac
     1201 gatcaattca tcgatttcat gatcgcgtaa ccagtcatcg agagaggttt tctgccgacg
     1261 gccgttatca aaaaaggcac tgtaactgtc aactaaagga ttttcgcctt tatggaacac
     1321 cgctgcgatc gctttttggt tcagtaacgg atgtaattgt gcgccttcac tgttctgcac
     1381 acagtgatct ggccagaagg tttgtggcaa accgtcgagt tggcctggcg tataaggctc
     1441 tacaccgtgc tgactggcaa aactgccgtg attcgccgga tgccagtcct ggctggcgat
     1501 aaccgcttct ccgcgcgact ggcaccagtc aataaggcgg ttagcaacct ccaccgtact
     1561 gtcaccttcc ggtacggcga gcgcgccacc agcacagaaa tcattttgta aatcgaccag
     1621 taacagggcg cgatggggca ttgcagtctc cttaatcatc cggcgtcagt tcgccgcgca
     1681 ggttttggct catggccttg cgaatggttt cagtatccag ttcctggctc agcaggtaat
     1741 gcagtttggt tagcgtggct tctacagtca tatctgcacc gccaataacg ccggcatggg
     1801 cgagggcgtt accggtggca taaccaccca tattcacttt accggacata cattgtgtca
     1861 ggttgaccac cacaataccg cgatcgctgg cttcttgtaa ttcctgcagg aaggctttgt
     1921 tttgtggcgc attacccacg ccataggagc gcagaatcaa tgctttcacc ggttggcgca
     1981 gaaaattgcg cacgacatca gcagaaatcc ccggataaat cgtcactacg ccaattggtt
     2041 gtggcgtgat tggatgaacg attaattccc cttcaccgtg cggggcgggt ggcgtattca
     2101 aacgacgaat atggatacca gcttccagta acggaggaag gtttggagag gcaaacgcat
     2161 caaaaccatc ggcatgggct ttggtagtgc ggttgccgcg atacaatcgg ttattgaaaa
     2221 agagcgttac ttcgttgatc ggataattcg ccgcaacgta caacgcattc agcagattaa
     2281 tttgtccgtc agagcgtaac tcagccagcg ggatttgtga ccctgtcaca atgaccggtt
     2341 taccgagatt ctcgagcatg aacgacagcg cagaggcggt atacgccatc gtgtcggtac
     2401 cgtgtagaat gacaaaacca tcatagtcgt catagtgcgc tttaatatct tcagcaatat
     2461 gctgccagtc ttccggcgtc atatcagaag aatccatcaa cggcgtatat tcatgaatgg
     2521 tgaaatctgg catctccggg cgatggaatt ccggcatcag cgccagttgg cgttgtagat
     2581 gacctgacac cggaatataa ccctgctcgg aacgctgcat cccgatggtc ccgcccgtgt
     2641 aggcgacgta aattgatttc ttttgcatga tattgatatg aatgatatcg aaagagcaaa
     2701 agtatatacg tgaggcagga ggagcgcacc tgatactatg cggataagga aatatgggtc
     2761 gagacagcgg gaagaggatt cgtcgaggta aaaaataatg tggccgacga tctgtcggcc
     2821 actcagtaca agacttaacg cacgttggcg caggtcaggc aaaacgcata acggttttgt
     2881 gggtcgttaa acgcggccag cttgtcgctt tcacttttaa cggtagaggc taccgaggca
     2941 agcggtgcag gtaacatggc ctggaacgca tctggcaaca ttgcccggac agaaccagac
     3001 atgttgtcca tcactttgtc gaagaaggtc ggttcatcaa cgtagtattc cagatgccac
     3061 tgtttcactt ttgccagctc tgctgctttg gcaaccgcat catcgaaatc cccgagacta
     3121 tcgaccagcc cgttagcttt tgcatcctga ccggtccaga cgtggccctg ggcgatttta
     3181 tcaatttgct ccggcgtcga atgacgcgca tcagcaacca gcgtgataaa gcgtttatag
     3241 ccattctcaa tgcttaattg catcatctgc tgcgcttccg gcggcagtgc cctggtgata
     3301 gaaacatccg ccagcggtga agttgagaca ccatcagtat gaacaccaat cgaatccaga
     3361 ctattttcta cggtggtgat cacgccgaag ataccgatag aaccggtcag ggtgctgggg
     3421 ttagccacaa tgtaattagc tggcgtggaa atccagtaac caccagatgc cgccatgccg
     3481 cccatcgata caaccacagg cttacccgct gcccgggctg ctgccagttc agcgcgaatc
     3541 acttcagacg cggtaacgct gccgcctggg ctattaacac gcaggacaat cgctttcact
     3601 ttcgggtcaa ggcgagcgtc gcggatttgt gccgcagtgg tatcaccgcc aacattcccc
     3661 tgagtttcct cgccatccat aattgcgcca ttagcgaaga cgacaccgat gctgtcaccg
     3721 gtatctgccg gcgttttcaa tgcgtaatcg taataactga tggcgcgata atttttatca
     3781 gtcttactcc agccgaactc tttggtcaga gctttttcga tttcggcact cgatgccagt
     3841 gcatcgacca gcttgttttc cagtgcatat ttcgcggtat cgccaccggt tttggttaaa
     3901 ccctcaagca acccttgcgc gccagggaat acctgctgag cagggatctg ccggttagcg
     3961 gcaacagtat tcagatagtt ttgccacagc tcaccaatcc agcggctgtc agcttcgcgg
     4021 gctgccggtg acatatcatc acgaataaac ggttcaacgg cagatttata cgtacccacg
     4081 cggaacacat gggtggaaac tttcagctta tccagcaacg atttgtagta cagaccgttg
     4141 gtagcaaaac cgtgcagatc aaccacgcct tgcggtgaca gccaaatttt attggcgaaa
     4201 ctggcgagat aatattgccc ctggctgtag ttctcgccaa cggcataaac cggtttcccg
     4261 ctgtcacgaa actctttcag ggctttgccg atgtactgca tagacggctg gtcgccgcct
     4321 gcgaagtttt tcagatccat cacgataccg gtgatattgc ggtcgtcctt cgcctggcga
     4381 atggtgttga cgatatcaaa cagtgagttt tcctgcagac gatcggaact ggcaccaagc
     4441 agctggcggc ttaatttact aaaccgctga gaactgtcgg gtttatcgac gatcacacca
     4501 gaaatgtcca gcagcagtgc gccacgactg gccgtttctt tcgaatcacc accactgacc
     4561 tgcatccaga tccccacacc aaccagtacg aggaaaataa agaacaggtt aagtaccatt
     4621 tcacggacga aattcagcag acgccacgtc catttaaaaa atccggcaat aaatcgccaa
     4681 agggttcgca tgtattctcc caacttaagg tcacacctgt cacaggcgca atagatgtgg
     4741 ttatcgtaat gacccaaccg gcacttgtca gcaggaattg cggactgtgc tgtaacaaaa
     4801 tccactctcg tgttaatttt gtgaataaat atcacgacag gagttaatca aatggatgca
     4861 ctcgaactat tgatcaatcg ccgtagcgcc tcccgcctgg ctgaacccgc gccaacgggt
     4921 gaacaactgc aaaacatcct gcgtgcgggt atgcgtgcgc cggaccataa gtccatgcaa
     4981 ccgtggcatt tttttgtgat tgaaggggaa gggcgcgagc gtttcagcgc cgtactggaa
     5041 cagggggcga ttgctgccgg tagtgatgac aaagctatcg acaaagcccg taatgcgccg
     5101 ttccgcgcac cgctcatcat cacggtggtg gcgaaatgcg aagaaaatca taaagtcccg
     5161 cgatgggaac aggaaatgtc tgccggatgc gcggtcatgg cgatgcaaat ggcagccgtt
     5221 gcccaaggat ttggcggcat ctggcgcagt ggcgcattaa ctgaaagtcc ggtagtgcgt
     5281 gaagcattcg gttgccgtga gcaggataaa attgtcggtt ttctctacct cggcacgccg
     5341 cagttgaaag catctacgtc gattaacgtc ccggacccga cgccgtttgt aacttatttc
     5401 tgataattgt cgcgaaactg tctggattgt gagcaaaggc gcaggaattc agacactctc
     5461 acttatcact tcacggaatg agggttacca tacccggatt gcattgacag gagatgtcca
     5521 tgagcgagaa ctcgattcgt ttgacccaat acagccacgg agctggttgc ggctgtaaaa
     5581 tttccccaaa agtgttggaa accatcctgc acagtgagca ggcgaagttt gttgatccga
     5641 atttgcttgt gggtaatgag acccgcgacg atgcggcggt gtacgatctg ggcaatggca
     5701 ccagcgttat cagtaccacc gacttcttta tgccgatcgt tgataatcct ttcgattttg
     5761 gccgcattgc ggcgactaac gccatcagcg atatcttcgc gatggggggc aaaccgatta
     5821 tggcgattgc gatcctcggc tggccgatta acaaactttc cccagaaatt gcccgcgaag
     5881 taaccgaagg tggacgctat gcatgccgtc aggcgggtat tgcgctggct ggcggtcact
     5941 ccatcgatgc gccggagccg atttttggtc tggcggtaac ggggatcgta ccgaccgagc
     6001 gggtgaagaa aaacagtacc gcacaagccg gatgcaaact gttcctgacg aaaccgctgg
     6061 ggatcggcgt tcttaccacg gctgagaaaa aatcactgtt gaaaccagaa catcagggac
     6121 tggcgacgga agtgatgtgc cggatgaaca tcgcaggtgc gtcctttgcc aacatcgaag
     6181 gcgtaaaagc gatgaccgac gttactggtt ttggtctgct gggccatttg agcgaaatgt
     6241 gtcagggcgc aggtgtgcag gcgcgcgttg actatgacgc gatcccaaaa ttgccggggg
     6301 ttgaagagta cattaagttg ggcgcagtgc ctggcggcac cgaacgtaac tttgccagct
     6361 acggtcatct gatgggtgaa atgccgcgtg aag
//
LOCUS       DAMZET010000207         6365 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.207, whole genome shotgun sequence.
ACCESSION   DAMZET010000207 DAMZET010000000
VERSION     DAMZET010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.207"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(39..1217)
                     /gene="setA"
                     /locus_tag="POB34_004242"
     CDS             complement(39..1217)
                     /gene="setA"
                     /locus_tag="POB34_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HDI5921093.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(1319..1450)
                     /gene="sgrT"
                     /locus_tag="POB34_004243"
     CDS             complement(1319..1450)
                     /gene="sgrT"
                     /locus_tag="POB34_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HDI5921094.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1539..3194
                     /gene="sgrR"
                     /locus_tag="POB34_004244"
     CDS             1539..3194
                     /gene="sgrR"
                     /locus_tag="POB34_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HDI5921095.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3228..3327
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3358..4341
                     /gene="thiB"
                     /locus_tag="POB34_004245"
     CDS             3358..4341
                     /gene="thiB"
                     /locus_tag="POB34_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HDI5921096.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4317..5927
                     /gene="thiP"
                     /locus_tag="POB34_004246"
     CDS             4317..5927
                     /gene="thiP"
                     /locus_tag="POB34_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HDI5921097.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLKWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5911..>6365
                     /gene="thiQ"
                     /locus_tag="POB34_004247"
     CDS             5911..>6365
                     /gene="thiQ"
                     /locus_tag="POB34_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein"
                     /protein_id="HDI5921098.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPIL"
BASE COUNT         1473 a         1752 c         1679 g         1461 t
ORIGIN      
        1 cggtcaaatg accgggcttt ccgctatcgt ccacgtcatc aaacgtcttt aacctttgcg
       61 gttaaaaata atgcgacaac agaaataacc gcaattaccc agtagacagc aaagtgcccc
      121 caactttgtg caattgctcc ctgaataacg ccagccagaa ttaccccggt agaaatactg
      181 ttagtaaata aggtggtagc tgcccccgct cttccaggca ttaaatcctg aaaccatagc
      241 atcccaatac ccgcaacaat gccgataaat acagcgttaa aaagttgcag cgtcatcaac
      301 gccatacggc tatgaaagaa aatcaatccg gtgtaaaaca gtactcctgc cgccactgct
      361 atgaccatca ttcgccgctt accataacgt ttgacatagt agccagccag aatcattgct
      421 ggtatttcca gtccagctgc cgtccccatc aggaaacccg ccagtttgtc tggcaatcct
      481 aactcgctac tgatccacaa cggcatatca ataatgtaca tggtgttgca ggtccacatt
      541 aacgtcgagg cgacaaataa catccgtacg ttactatcct gccagccacc ttgcattgat
      601 aaagcatttt ccgacggcag ttctacccgc gccacagacg gaagcataaa tgcaatcaat
      661 accagactga gtgtgaatat cccggcggca atcgaaaaca tcaccgtaaa gccgtaattc
      721 aacgccagca taaaggccaa cggtggaccg ataacccatg ccagagaaag ctgcgcacgc
      781 atcaccgagc taaacatcac cacttctcgc gccgagttat ccgcatattc ccgcgccaga
      841 gcaaataact gtggcattgc cgtattggcc agagatgcca gaagcacacc acaggtgata
      901 agcgtcagat aatgacgatt aaatgcaaac aatagcgcat tgccgatagc catcaaacag
      961 caaaatataa tcagttttcg ccgatcgccc tgactgtcag aacgttttgc caaccagagg
     1021 cttacgccga tcccagcaat agcattcacc gtataaaaga gtccgatcca gaaaggttgc
     1081 gcgccaacct cacgactcag aaataagctc aatgtaggag cctgtagcgc cccggccacc
     1141 cccatcataa aagcgaccag cataaatgcc gcgtaaacac cgttcatacg gcgagccatc
     1201 gtcattatcc agatcatacg ttcccttttt agcgcggcga gaataaaaaa aaccagcagg
     1261 tataatctgc tggcgggtga ttttacacca atactcagtc acacatgatg caggcaagtc
     1321 aactttcaga attgcggtca tcccactgca tcagtccttc aagcatggtt aatcgttgtg
     1381 ggacgcttaa ccaacgcaac cagcacaact tcgctgtcgc ggtaaaatag tgctgataaa
     1441 actgacgcat ggggcacctc cttgcttcat cgttaggaat tattgactta atatagggaa
     1501 aataaaattg ctgtcttttg cgcaggagtt ccccttttat gccatctgct cgtctgcaac
     1561 aacagttcat ccgcctgtgg caatgctgcg agggtaaatc gcaggacaca acgctcaacg
     1621 aactggcagc gttattgagc tgctcgcgtc gtcatatgcg taccctgctc aacaccatgc
     1681 aggatcgcgg ctggctgacg tgggaagcgg aagtcgggcg cggtaaacgc tcgcgtctga
     1741 cattcctcta taccgggctg gcgcttcagc aacagcgggc ggaagacctg ctggagcagg
     1801 atcgtatcga ccaactagtg cagttggttg gcgacaaagc gactgtgcgg caaatgctgg
     1861 tttctcatct gggccgcagc ttccgccagg ggcggcacat cctgcgcgtg ctctactatc
     1921 gtccgttgcg taatctgcta cctggcagcg cattgcgccg ttccgaaacc catattgccc
     1981 ggcaaatctt cagttcgcta acgcgcataa atgaggaaaa tggggaactg gaagcagaca
     2041 tcgcccacca ctggcagcaa atatcaccgc ttcactggcg tttctttttg cgtccaggag
     2101 tccattttca tcatggtcgt gaactggaaa tggacgacgt gatcgcctct ttaaaacgaa
     2161 tcaatacgct gccgctctat tcgcatattg cggacattgt gtcgccgacg ccctggacgc
     2221 tggatatcca tctcacgcaa ccggaccgct ggttaccgtt actgctggga caagttccgg
     2281 cgatgatcct gccgcgcgaa tgggaaaccc tcagtaactt tgccagccat cccatcggca
     2341 ccggtccgta tgcggtgatc cgcaacacca ccaatcaact gaaaattcag gcattcgatg
     2401 acttcttcgg ttaccgggca ttaatcgatg aagttaacgt ctgggttctg ccggaaattg
     2461 ccgacgagcc agctggaggt ctgatgctaa aaggtccaca gggcgaggaa aaagagattg
     2521 aaagccgcct ggaggaaggt tgctactatt tactgttcga cagccgcacc catcgcgggg
     2581 cgaatcagca agtcagggac tgggtaagct atgtgctttc tccaactaat ctggtctatt
     2641 tcgctgagga acagtaccag caactgtggt tcccggctta tggactgctc ccccgttggc
     2701 accatgcccg caccataaag agcgaaaaac cggctggcct ggaaagcctc accctgacct
     2761 tttatcagga tcacagtgag catcgggtga ttgccgggat catgcagcag attctggcaa
     2821 gtcaccaggt cacgctggaa atcaaagaga tcagctacga tcagtggcat gaaggagaga
     2881 tcgagagcga tatctggctt aatagcgcca actttacgct gccgctggat ttttcgctgt
     2941 tcgcgcactt gtgcgaggtg ccgctgctcc aacactgtat tccgatcgac tggcaagccg
     3001 acgccgcccg ctggcgcaat ggcgaaatga acctggcgaa ctggtgccag caactggtcg
     3061 ccagtaaagc aatggtgccg cttatccacc actggctgat cattcaggga caacgcagta
     3121 tgcgcggcct gcgtatgaat accctcggct ggttcgattt taaatcagcg tggtttgcgc
     3181 cgccggatcc atgattgctg gtagataaca aaatcactac actaacgccg ttctcaacgg
     3241 ggtgccacgc aaacgcgtgc gctgagaaaa tacccgtcga acctgatccg gataacgccg
     3301 gcgaagggat ttgaggctcc ttctcaagtc ctttgccact cttttttgag gtgcaaagtg
     3361 ttaaaaaaat gtcttcccct gctgttgctg tgcacagcgc ccgttttcgc taaacccgtt
     3421 ctgactgttt atacctacga ttccttcgcc gccgactggg ggcctggtcc aaagatcaaa
     3481 aaagcttttg aagccgactg taactgcgaa ctgaaactgg tggcgctgga agatggcgtt
     3541 tcgcttctca accgtctacg gatggaaggc aaaaatagta aagccgatgt ggtgctgggg
     3601 ctggataaca atctgttaga cgccgccagt aaaaccggac tatttgccaa aagcggtgtg
     3661 gcagcggatg ccgttaacgt tcccggcggc tggaataatg acactttcgt cccgtttgat
     3721 tacggctatt tcgccttcgt ctatgacaag aacaaactga aaaacccgcc gcaaagcctg
     3781 aaagaattgg ttgagagcga tcaaaactgg cgggtgattt atcaggatcc gcgcaccagt
     3841 acgccggggc tgggcctgct gttgtggatg caaaaagtct atggcgatga cgccccacaa
     3901 gcctggcaaa aactggcgaa gaaaaccgtc acggtcacca aaggctggag cgaagcctac
     3961 ggcctgtttt taaaaggtga aagcgatctg gtactgagtt acaccacctc tccggcttat
     4021 cacattctcg aagagaaaaa agataactac gccgccgcga actttagcga aggtcactat
     4081 ctgcaggtgg aagtcgccgc ccgcaccgct gccagcaagc agccggagct ggcgcaaaaa
     4141 ttcctccagt ttatggtttc tccggctttt cagaatgcga tcccaaccgg taactggatg
     4201 tatccggtgg caaatgtcac gctgcctgcc gggtttgaac aattgaccaa accggccacc
     4261 acgctggagt tcacaccagc tgaagtggcg gcacaacgtc aggcatggat tagcgaatgg
     4321 caacgcgccg tcagccgtta attcccggct ggttaattcc aggtgtaagc gccgccacgc
     4381 tggtggtagc ggttgcgctc gcggcgtttc tcgccctgtg gtggaacgcg ccgcagggta
     4441 actgggtggc agtctggcag gacagctacc tgtggcatgt ggtgcgcttc tccttctggc
     4501 aggcgtttct ctcggcgcta ctctctgtcg tacccgcgat attcctcgcc cgcgccctct
     4561 atcgcaggcg ctttccgggt cggctggcgc tgttgcgtct gtgcgcaatg accttgatcc
     4621 tcccggtgct agttgctgtt ttcggcattc ttagcgtcta tggtcgtcag ggctggctgg
     4681 catcgctctg ccaatcgctc ggtctgaagt ggaccttttc gccctacggc ctgcaaggta
     4741 ttttgctggc gcacgtattt tttaatctgc cgatggcgag ccgcttatta ctccaggcac
     4801 tggaaaacat tcccggcgaa caacgtcagc ttgccgccca gcttgggatg cgcggctggc
     4861 attttttccg cttcgtcgaa tggccgtggt tacggcgaca aatcccgccg gttgctgcac
     4921 ttatctttat gctctgtttc gccagcttcg ccaccgtgct atcactgggc ggcggtccgc
     4981 aggcgaccac tatcgagctg gctatttatc aggcgctgag ttacgactac gatcctgccc
     5041 gcgcggcgat gctggcgctg atccagatgg tgtgttgcct tgggctggta ctgttgagtc
     5101 agcgattgag taaggccatt gcgccaggca ccacgctgct gcaaggctgg cgcgacccgg
     5161 acgatcgtct gcatagccgc atttgcgaca cggtgttaat tgtgctggcg ctgctgctgt
     5221 tgctgccacc gttactggcg gtgatcgtcg atggcgtaaa tcgccagttg ccggaagtgc
     5281 tggcacaacc ggtgctgtgg caggcgctgt ggacctcgtt gcgtattgcg ctggcggcag
     5341 gtgtattgtg cgtagtgctg accatgatgc tgctatggag cagtcgcgaa cttcgggcgc
     5401 ggcagaaaat gctggcgggt caggcgctgg agatgagcgg catgttgatc ctcgccatgc
     5461 cggggattgt gctggctacc ggcttctttt tactgctcaa caacaccatt ggcctgccgc
     5521 aatctgctga cggcattgtg attttcacca atgcgttaat ggcgatccct tatgcgctga
     5581 aagtactgga aaacccgatg cgcgatatca ccgcccgcta cagtatgttg tgtcagtcgc
     5641 tgggcattga aggctggtcg cgcttaaaag tggtcgagct gcgcgccctg aaacgtccac
     5701 tggcgcaggc gctggctttt gcctgcgtgc tgtcgattgg tgattttggc gtagtggcgt
     5761 tgttcggtaa cgatgatttc cgtaccctgc cgttttatct ctaccagcaa attggctcct
     5821 atcgcagcca ggacggtgcg gtcaccgcgt taattctgct actactctgt tttctgctgt
     5881 ttaccgtgat tgaaaaacta ccggggcgaa atgttaaaac tgactgatat cacctggctt
     5941 tatcaccatt tgccgatgcg ttttagctta acggtagaac gcggcgagca ggtggcaatc
     6001 ctcgggccaa gcggcgcggg taaaagtacc ctgctgaatt tgatcgccgg ttttctgacg
     6061 ccagccagcg gttcgctgac tatcgacggc gttgatcaca ccacgacgcc gccgtcacgc
     6121 cgtccggtat cgatgctgtt tcaggagaac aacctgttca gccacctgac ggtcgcgcag
     6181 aacatcgggc tggggcttaa tccgggattg aagctgaacg cggcgcagca ggagaaaatg
     6241 cacgctatcg cccgccagat ggggattgat aatttaatgg cgcggttacc gggcgagctt
     6301 tccggcggtc agcgacagcg cgtggcgtta gcgcgttgtc tggtgcgcga acagccgatt
     6361 ttatt
//
LOCUS       DAMZET010000208         6353 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.208, whole genome shotgun sequence.
ACCESSION   DAMZET010000208 DAMZET010000000
VERSION     DAMZET010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.208"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..329)
                     /locus_tag="POB34_004248"
     CDS             complement(<1..329)
                     /locus_tag="POB34_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDI5921099.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGP"
     gene            complement(360..1202)
                     /locus_tag="POB34_004249"
     CDS             complement(360..1202)
                     /locus_tag="POB34_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDI5921100.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(1189..2070)
                     /locus_tag="POB34_004250"
     CDS             complement(1189..2070)
                     /locus_tag="POB34_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDI5921101.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(2091..3383)
                     /locus_tag="POB34_004251"
     CDS             complement(2091..3383)
                     /locus_tag="POB34_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI5921102.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELVEQH"
     gene            complement(3397..5076)
                     /gene="ycjM"
                     /locus_tag="POB34_004252"
     CDS             complement(3397..5076)
                     /gene="ycjM"
                     /locus_tag="POB34_004252"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HDI5921103.1"
                     /translation="MKQKITDHLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YQQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTINSSVMRIQRSN
                     ADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            complement(5289..5603)
                     /gene="pspE"
                     /locus_tag="POB34_004253"
     CDS             complement(5289..5603)
                     /gene="pspE"
                     /locus_tag="POB34_004253"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HDI5921104.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(5678..5899)
                     /gene="pspD"
                     /locus_tag="POB34_004254"
     CDS             complement(5678..5899)
                     /gene="pspD"
                     /locus_tag="POB34_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HDI5921105.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(5908..6267)
                     /gene="pspC"
                     /locus_tag="POB34_004255"
     CDS             complement(5908..6267)
                     /gene="pspC"
                     /locus_tag="POB34_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HDI5921106.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(6267..>6353)
                     /gene="pspB"
                     /locus_tag="POB34_004256"
     CDS             complement(6267..>6353)
                     /gene="pspB"
                     /locus_tag="POB34_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="DNA-binding transcriptional regulator; acts
                     together with PspC to induce psp operon during infection
                     with phage, exposure to ethanol or osmotic shock; forms a
                     complex with PspA and C; PspC is required for PspAB
                     binding; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HDI5921107.1"
                     /translation="ADEAKRMRERIQALESILDAEHPNWRDR"
BASE COUNT         1639 a         1646 c         1514 g         1554 t
ORIGIN      
        1 gggccgtagc cgcatacgct gtcgcccacc gcgtagtcgg taacgtcgct gccgcactcg
       61 ataatgtcgc caaccaccat gttgccaagc tggaatttgc caaactcaat gccgcgcggc
      121 gcatctgccg gacgcggagt gaacatctgc cattcgccgt taaagtcttc atcgataaac
      181 gggctggcgg cgcggaagtc gaccacttcc gttccgtgtt tcggtgcgcc gaaacgcacg
      241 cggattttca cttcattggc taaaatcgcc cgatcttcat actcaaccag cgcagcaaca
      301 cgcggtgctg tggctactaa ctttttcatt taaaactcct tgtaaagaaa ctggccgctt
      361 tagcccttca cgccaccggc ggtcaaacca ctcttaataa aacgttccga aagcgcatac
      421 ataatcacca ccggcaatgc ggtcaccagt gaggccgcca tcatccgtcc ccagatgtaa
      481 tctggcgtac tgaacagcgc gttcaggccc accggtaagg tgaaattgct ggcgctggag
      541 aggaagatcg acgcaaacag atagtcgttc cacgccacca taaagcagta gacgaacacg
      601 gatatcagcc cggacatcgc caacggcacg gtgatgcgga agataatttg cagacggttg
      661 aggccatcca tcatcgccgc ttcttcgatc tcatcgggga tggtgtcgaa gtagcttttc
      721 agcataaaca cggcggtggg cagagtttgc gtcaccatgg tgatgatcag cgccatctca
      781 gtgtcgtaaa tgcccagcgc ggtgatgatt ttgaacagcg gcaccaccag caaaatgcca
      841 gagaacatat acaccgtgta aaagctggcg ttgatggtca tccgaccttt aaagcgcagg
      901 cgggaaagcg cgtaagcccc aagaatgccg aggaataccg ccaccacgga tgaaaccaca
      961 gacaccacca gactgttacg gaagtagtcg acaaacggga aaatcatcgg gttaaaaatg
     1021 tcgacgtaat gctccagcgt ccactgctgc ggcagcagcg taggatgcag tgagatcgcc
     1081 tctttcgcgc ccttgaacga ggtcatcagc atcacaaaaa atggaaacag cgtgatgatg
     1141 agaaacagcg ccagcccgca gtaaaaaccg atgcgactga gtgtgcgttt atttgttgcc
     1201 attgaggttc acccgtttcc tggtcagcag aatgacagcg aaaataatga tgaagagcac
     1261 caccgaaatc gccgccgctt tccctaagtc attaaaggca aaggcggttt tgtagagata
     1321 gacgccgaga atatcgacct tggtggtcag caaataaaca tccgcgaaca tgtagaacat
     1381 ccagatggtg cgcagggtca ccaccgtcgc caggaccggc ataatcgcgg gcagcgtgac
     1441 gatacgaaac cgttgccagg cattagcgcc atccatctct gccgcttcgt acagcgattt
     1501 gtcgatggtc tgcaaaatcg ccagaaacga gataaaggca tacgggaagt agcgccagat
     1561 ggcgaacagc accaccagca caaagctact gcccggatta tcgaaccaca gcggtgcctg
     1621 ctcatagaga tgcagaagat cgacgccgag gtagttcaca atgccgtagc cgttgttgaa
     1681 catgtatttc caggcgaaca ccagcgaaat ggacggcgtt acgtaggaga ggatcaccag
     1741 cgaacgcgcg gttttgcgca ggcggaactc gcgattgaaa aacatcgcca ccgccagccc
     1801 gagcacggtg ctgcccgcca ccaccagcgc ggtgtaccag accgtcatcc acagtgagtg
     1861 ccagaagccg ggatcggaga ggatacgcac atagttgctc accccaacaa aggttgactc
     1921 gatgttggga ttgagcggca gacgtaaaaa actaatttcg atattcgaca ccatcggcca
     1981 cgccaccaga ccgcccagca gtaataagct gggcgcgaga agcaacagcg caaagggcat
     2041 atcggaacga cctgaaaaaa gcctgttcat tgcggattcc tgtcctgggt ttagtgctgt
     2101 tcgaccagtt catccagctt tttctggctc gcttgcagcg tgccagggag atcggcttta
     2161 cccacggtga cgttatgcac catggagctc accacgccag accccgtcac atcacccatg
     2221 cgggtaaagt ttttatcccc tactgcacca aagacctgaa tatttggcag ttcaccgatt
     2281 agctgattcg gtagttcacc cagcgcctta ataacgtcgt tgtctttcca ggtggcggta
     2341 gtgacaaccg ctttattcac cggcagcgca gcacctggcg acatcatcac ccagtcggca
     2401 atgttgtctg cctgctccat aaaggtgaca aatttttctg ctgcttccgt ctcttcggtc
     2461 ttttgcccgg cggtaatggt cagcgaggtc aacatgccgt agaccgcaga gtttttctcg
     2521 gttggcacca cgaaaccgac gtttttcggg tcgccttctt taatcacagc cggaaggata
     2581 taggtggagt aaatcgccat cggcgcggtg ccgttcataa aggcgtcttt cacttccatg
     2641 atgtcgttag aacccggcat agtgttggca gcaaggtcgc ggtaataggt cagtgcctgc
     2701 atcatctctg gtgtatcaag ggtgattttg ccttcggcgt taaagacgtt agcctggttg
     2761 gataacgcaa actgggagaa ggattgttcc gtcaacacgc tttctgctgt aggcagcgca
     2821 atgccgtatt ttttattcgc cgggtcattc agtttctgtg caacgtccag cagctgttgc
     2881 cagtttttcg gctcctcaag ccccgctttt gccagcacat ctttgcgata ccagataccg
     2941 ccaatccagg cgctgacagg aacaccggtc catgcgctac catcttcggt acgcacaata
     3001 cgcagtacgc catcgtaaaa cgcgccttca ccaacgttgc tgatgactgt ggcaaccgct
     3061 ttgcgatcga taagctgctc tttatccatc actttggcgt agtcatggct ggtttcgatc
     3121 acttccggca gcgaaccgct acgtgaaaga gtaatgactt tagtgttata ggcatcttct
     3181 tccacgggca cttgcttaac ggtgatgcca gggttttctt tttcaaaacg ggcaatcaat
     3241 ttactgataa cggcctggcg ctcctgctcc accgaagaat gcataaattc gatggataca
     3301 ttcgttttat tttcttcttt acagcctgaa atcagggcgc aggaaaccag tgctgataac
     3361 agcacgattt ttgatttaat catgagatgt tcctttttat ttaatccaca taacctgcca
     3421 ggggcgcagc gttatttcat tacccaatat atcaacttca ctaatcagat cccgaccatg
     3481 cagattagta atatttacat gctgaatatt tttactgaca ttaaacaatc cagtcagaca
     3541 attaccatca gcgttacttc tttgaatacg cattacggat gaattaatcg tatcaatggt
     3601 aaaattatta tccggatgaa actcgttatg gctgcgacga agtgtaatta aacgcgacaa
     3661 ctcatgatat accgcatgcc ttaatgtagc ttcatcgttc agttctcggg ttatctcttt
     3721 actgtgatat tttttacggt taatcgcacg gttatatccg agtttttcga cacctgcgta
     3781 atcattacgc gagccaagaa tactttgaat atatatcgct ggcacaccgg ggaaacttaa
     3841 caaaatcgca tgggcgagaa taaacctggc gcaacgttct tcatcgctac tctcacggcg
     3901 gcttaacgca tccatatagg tcacatttat ttcatacgga ctgcgtgtac cgtcgggatt
     3961 atttttccag tttactaatg caccttcctg ctgtaacgcc tcgaccagct ctaatatttc
     4021 gctttcaggc aacaagcccc gtagcgggtt tagcccgatg ccatcgtgag aggcgaggaa
     4081 gttaaaccag gtggtgttgc tggaaggtag tgtcaggttt tgcgcccacg cacaaagcgc
     4141 ctcaacgttc tgtttttgca ccgcatgcag caccagtggc ggcagcgaga actggtacac
     4201 catatgtgct tcgtcatcgc ctgcgccaaa gtaagcaatg ttgtctttat gcggaacatt
     4261 ggtctcggta atgatcactg tacctggcgc aacgttatca ataatcgacc gtaacagttt
     4321 gataatcaga tgtgtttttt ccagatggat gcagcttgtt cccggctctt tccacataaa
     4381 gccaacggca tccaggcgga catactcagc acctttcgca aggtaacaga gtaaaacatc
     4441 caccatcgcc agcaacactt cagggctacg gtaattcagg tcaatttgat cgtcactaaa
     4501 ggtggtccat aaatggcgcg ttgaatgatc gcgcatctgg aatggcgtta ataacggtaa
     4561 cgcacgcggg cgagtgacgg cgctgagatc ggtttgcggg tcaacggcaa taaaaaaatc
     4621 ttcaaaacct ggatgctgtt gtaaatagtt tttaaaccat tcactttttg ccgacatatg
     4681 gttgcagaca aaatcaaaca ttaaatgact gcattcaccg agttgctgaa tatcctgcca
     4741 ctcccccgct tcactggcga cctgttgata atcaattacc gaaaagccat catcagatga
     4801 ccatggataa aacggcaaca aatgaacatg tgaaaaaatg ctttgcagcc attgatggta
     4861 aaactgatta aatgtgggta atggttttaa atcattgctg tgaaattgat cggcataggt
     4921 aattaacacg acatcacttt catcccagtg ttttttacgt cgctgtgtaa ttaatcgttt
     4981 cgcactctca agacgcgtta caagtttctg taaatgagtt gcggtaaatg ttccaccgta
     5041 gatttcgtcc aggtgatccg taattttctg tttcatgttg cctttccgtg gtagcggtcc
     5101 catgaaacga gagactactc cgcagaaaat aaactgtcaa ttgagcacaa gggttttagt
     5161 cacgcaggaa atcatttttt accagggaaa aagcgtattt gtgagctgac gcaaataatc
     5221 tatggggatg taaataaggt gtgacagaaa aaaaaacggc gcataagcgc cgctcatggt
     5281 gaattctttt aacctttgac cttcggcatt gcgatgtctt tcaggccacc ggcgttctca
     5341 acgtgggtat atcccatctc gctaaggatc tcttttgctt gccctgactg gcgtccggca
     5401 ttgcaataca ctttcacggt gtcgttttta tccggaacgg cggtggcaat gcgctctttc
     5461 acttccttca ggggaatatt gatggccccc tgaacgtgct cttgctgata ctgctctgga
     5521 acacgaacat cgatccagtg ttcagcggcg aaaacgggca gtgaaaacac cagtgccaga
     5581 gcaagtaagc cttttttaaa catgtaagcg tccttctatg gatgaaaatt gccatccatt
     5641 ataggcctga gatttaacaa tttttgcgaa actcccctca ccttttataa cgctgtgcca
     5701 gtttattagc agcccgactt aacagcggtt ccagcgccac agccagcaac attttcagcg
     5761 gtcggcgagc aactgatttt atcgcccagc ccgctacacc cgccgggcca tagcgcagtg
     5821 cggtaagaag taccagcttg cctgctaatt tgaaaccagg ctttaccttt tgcccggcct
     5881 gttgccagcg agtattcata actttcctca cagttgacgg aaacggctac gtaacgtgaa
     5941 agtatcggaa gtgacataac gttccatctc gcgtaaacgc gtttcacttg ccgccagttc
     6001 acggtcgact tcatccagca attcgctgct ggaaggtagc tgctcaccaa aggccatgtt
     6061 gtccggcatt ggatcaagcg caaatgacaa aatgatgtaa gcaaccaggg taaacagcgc
     6121 cagaccgaag aaaatcgaca gcaccaccag gatacgcacc agttttaccg gtacatcaaa
     6181 atagttggca atcccggcgc agacgccgcg gaccatgccc tgctgtggaa tacgccataa
     6241 ttttttattg agattaatgc ccgccattag cgatccctcc agttcggatg ttctgcatca
     6301 agaatggatt ccagcgcctg aatacgttcg cgcatccgtt ttgcttcatc agc
//
LOCUS       DAMZET010000209         6292 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.209, whole genome shotgun sequence.
ACCESSION   DAMZET010000209 DAMZET010000000
VERSION     DAMZET010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.209"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1619)
                     /gene="poxB"
                     /locus_tag="POB34_004257"
     CDS             complement(<1..1619)
                     /gene="poxB"
                     /locus_tag="POB34_004257"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HDI5921108.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKL"
     gene            complement(1752..2720)
                     /gene="hcr"
                     /locus_tag="POB34_004258"
     CDS             complement(1752..2720)
                     /gene="hcr"
                     /locus_tag="POB34_004258"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HDI5921109.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(2732..4384)
                     /gene="hcp"
                     /locus_tag="POB34_004259"
                     /gene_synonym="priS"
     CDS             complement(2732..4384)
                     /gene="hcp"
                     /locus_tag="POB34_004259"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDI5921110.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(4528..5427)
                     /gene="lysO"
                     /locus_tag="POB34_004260"
     CDS             complement(4528..5427)
                     /gene="lysO"
                     /locus_tag="POB34_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HDI5921111.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(5922..>6292)
                     /locus_tag="POB34_004261"
     CDS             complement(5922..>6292)
                     /locus_tag="POB34_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HDI5921112.1"
                     /translation="DAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGTT
                     DKFAPAGFAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVV
                     PIVGGIIGGLIYRTLLEKRD"
BASE COUNT         1575 a         1681 c         1655 g         1381 t
ORIGIN      
        1 agtttgatct gcggtggaat ggctaactct tctttggcga ccaccacatc caccaacacc
       61 ggaccgtcga tagagaaggc gcgttgcagg gcttcatcaa cttcagacgc tttttctaca
      121 cggatacccg taatgccgca cgcttcggca atgcgggcaa agtttgtgtc gtgtagttcg
      181 gtgccgtcag tcaaatagcc accagctttc atctccatcg ccacaaagcc cagcacgctg
      241 ttgttaaaga cgacaatttt cactggcagt ttcatctgca ctactgagag gaaatcgccc
      301 atcaacatgc taaaaccgcc atcgccgcac atggcgacca cctgacgttc tggctctgtc
      361 gcctgcgcac ccagcgcctg cggcatggcg ttagccatcg aaccgtggtt aaacgaacct
      421 aacaggcgac gcttgccgtt catttttaga taacgtgccg cccacaccgt tggcgtacca
      481 acgtcacagg tgaaaatagc gtcatcggcg gcaaaatgac taatttgctg cgccagatat
      541 tgcgggtgaa tggctttctc gctcggttta gctaaatcgt ccagcccttt gcgggcgtcg
      601 cggtaatctt ccagcgcttt atccagaaac ttgcgatcgg ctttttcttc caccaatgga
      661 agcaatgcac gcagagtcga cttgatatcg ccgaccagtg ccatatccac cttgctgtga
      721 gcgccgatgc tggctgggtt gatatcaatc tgaatgattt tggcatcggt cgggtagaag
      781 gcgcggtagg gaaattgcgt gccgagtagc actaacgtgt cggcgttcat catggtatgg
      841 aaacctgacg agaagccgat taacccggtc attccaacat catacggatt atcgtattcg
      901 acatgttctt taccgcgcag ggcatgaaca ataggcgctt taattttccc ggcaaactca
      961 actaactctt tatgcgcccc cgcgcagccg ctgccacaca tcagggcgat attgctggaa
     1021 taacgcagca gttgcgccag tttgcgtaac tcttcttctt ccggcgtcac gactggttgt
     1081 ggcgcatgat accagtgcgt ggttgcccct tctggcgcag gttttaacgc cacgtcgcct
     1141 ggtaacacga caaccgaaac gccacggtta agcaccgctt tgcgcatggc aatcgccagt
     1201 acttgtggga tctgctccgg gctggaaacc agctcgcaat agtgactaca ttcgcggaat
     1261 agctcttgtg ggtgggtttc ctggaaatag ccgctgccaa tttcgctgga gggaatatga
     1321 gcggcaatcg ccagtaccgg aacgtgattg cggtggcaat cgaacaggcc gttgattaag
     1381 tgcaggttgc cggggccgca cgatccggcg cagaccgcca gttctccgct aagttgtgct
     1441 tcagcgccag cggcaaaggc cgccacttct tcgtggcggg tggacatcca ctcgatggtg
     1501 cccatgcgat taagactgtc actaagaccg ttcagagagt cgccagtgac tccccagatg
     1561 cgtttcaccc ctgccgattc gagtgttttg gcgatataag ctgcaaccgt ttgtttcatg
     1621 gttctccatc tcctgaatgt gataacggta acaagtttag ttcatctgac ggagggggaa
     1681 gggatgggag agaaaggagg cactaacggt taaatagccc gatgaaagga atatcatcgg
     1741 gtataaggcg attatgcgag aaccaaatcc ccctgcggat ggcaggagca ggccagtacg
     1801 taaccttcag cgatttcggc gtcggtcagc gtcattgtac tgctcaccgt atattcaccg
     1861 gaaaccactt ttgtcttaca gcagccgcaa acacccgcac ggcaggcagc gacaaccgga
     1921 acgttattgc tttccagcgc ctccagtagc gtggtgccaa ccggggcgta aaattctcgt
     1981 gccggttgca gtttggtgaa tttcagaccg ctggtcgccg cttctgctac tggggtgaag
     2041 aatttctctt taaagaaacg cgtcacgccg agcgctttca cttcctgctc tacccaatcc
     2101 atatacggag ccgggccgca ggtcatcacg gtacgtgaag ctaagtcagg tacacctgcc
     2161 agcagttcgc gagtgagacg accagcgata aagccttcgg taacgttatt ttctgccacc
     2221 agcgttaccg gatagttacg ccactcatcg gcgaaaataa catcctgcgg cgtacgcacg
     2281 ttgtagatca cccgcacatc ggcctgtgga cggttcttcg caagccagcg acgcatcgac
     2341 ataatcggcg tgacgccgca gcctgccgcc agcaacagga atttatcttc tgctttatcg
     2401 tcgcaggtaa attcccccat cgcgtccgaa agccagagat aatcaccgcg ttttacatcg
     2461 cgcgtcagcc actgggagcc gacaccgtca tcaatccgcc gcacggtcag ggtgatatat
     2521 tcactcacgc ctggcgtgga ggaaatggtg taagcacgca gcgtttccgc tgagtttcgc
     2581 acgctgacca gtgcatattg cccggcgcga tatgggtagt aatcgtggca aatcagggaa
     2641 atcgtccaca catccggcgt ttcttgcgta atgtgatgaa cttgcatccg ccacgggcat
     2701 tgattcgttg gcatcgtcat cgacaaactc cttacgcgct caacagttgc ttcatgtctt
     2761 cttcaacagt ggtgatagaa cgcaggccaa atttctcgtt cagcaccgcc agcaggtctg
     2821 gtgtcaggaa accaggtgca gtcgggccgg tgacgatatt tttcacgccc agagaaagca
     2881 gcgtcagcag aatgacgatc gctttctgtt caaaccagga gagcaccagc gacagcggca
     2941 gatcgttgac accgcagccc agtttctctg ccagagtgac agccagaata atcgctgagt
     3001 aagcatcgtt acattgacct gcatctacca gacgcggcag accttcgata tcgccaaact
     3061 caagtttgtt aaagcgatat ttaccacagg cgagggtcag gatcaggcag tcatccggca
     3121 cgctggtggc gaaatcggtg aagtagtggc gctcgccgcg tgcgccgtca cagccaccaa
     3181 gcaggaagat atgacgcagt ttttcacggc tcaccagatc aatcagcgta tccgctgcgc
     3241 caagcagcgt ctggcggcca aagcccacgg tgatcaggtg cggaatttcg ctgtacggga
     3301 agcccgccat ttgttgcgcc tgggcgatga ccgcagaaaa atcttcgcca tccagatgac
     3361 gcacacccgg ccagccaacg atgctgcggg tccagatacg atcgtcataa gcgcctacgg
     3421 ttgggtcgat gatgcagttc gaggtcatca cgatggggcc agggaaacga gcgaactcca
     3481 cttgctgatt ctgccagccg ctgccgtagt taccgaccag atgcttgaat ttacgcagct
     3541 ccgggtagcc atgcgcaggc agcatttcgc cgtgggtgta aacattaacg cccgtgcctt
     3601 cggtctgttc cagcaggttg tagagatctt tgagatcgtg accggaaatc aggatgcatt
     3661 tacccgccgt cgctttgacg ttgacctggg ttggcgtcgg gtgaccgtat ttaccggttt
     3721 cgcctgcatc cagaatgctc atcactttga agttcatctg gccgatttcc attgaacact
     3781 caagaagcgc gttcatatcg gcaggccagg tccccagcca cgccatgatt ttatgatact
     3841 gggcataaat atcgttgtcg tattgaccga gaacatgcgc gtgttccata taggccgctg
     3901 cacctttcag gccatacagg cacagcagac gcaggccgag aatgttttcg ccaatcgccg
     3961 ctttatcttt gttaggggta aattctgctg cctgacgttg cagctcgccg agatcgtcgc
     4021 tcaccagttg caggtcagcc atcgggttat cgacgcgcgc gttggcatct acagccaggc
     4081 attgcgcttt cagcgcctcg cgcagggcaa tcgcttcacg agcgtagccg acaatacgcg
     4141 gagaatcgaa gttaacgttg gtcagggttg agaaaaaggc acgtggcgcg aagctgtcaa
     4201 catcgtggtt gatgatgccg tattcacgcg cttttaccgc ccaggcagaa agcccttgca
     4261 gcgccgcgat gagtaaatcc tgaaggtcag aagtttccgc cgttttacca cacatcccct
     4321 gcgcgtatga gcagccgttt cctgccggag tacggatagt ttgttcacat tgcacacaaa
     4381 acatgatcac accttttaaa gttatattta atatacatgt ttaaggttaa gacgcttaac
     4441 gcggggataa aagggatttt tcatgcaact ttaagggaga ttgatttagc gcaattttgg
     4501 cggcagggat ctaccgccag agaggtatta cgcagagaaa aaggcgatga ggatcggcac
     4561 taacaggcta agaataaaac cgtgaacaat tgccgccggg accatatcca gcccgccagt
     4621 acgttgaaga acgggcaggg tgaaatccat tgatgtggca ccgcataagc ccagtgcagt
     4681 agagcggctg cggcgaatca gcccagggat caacataata gcaatcagtt cacgggccag
     4741 atcattaaaa aacgccgcgc tcccgattac cggaccaaaa gattcggtca ataaaatacc
     4801 ggaaagagaa taccagccga aaccggaggc cattgccagc gcggtattga tggggagatc
     4861 aagaataaag gcgttaatta aaccaccaat taatgaactg acaaccacca ccacggcgac
     4921 aatcattccc cggcgattaa ggacaatctg ctttaaggtc atgccattat tgcgcaactg
     4981 aataccaacg aggaaaagta gcaaaattaa cgtgtattca ctggcttcgg tcgcgtgttg
     5041 taagaaagcc agtccactca gaccaatggc aaaaccaatc actactacgc cgcacagttt
     5101 tagcgactcc agcgccatcg caatacgcga cgggagtttt tcttgctgat ggtggttgcg
     5161 ccacggcagg cctcgctcca gccacatcag ggcggcaata ttacacagta aaataacggt
     5221 aatactgacg gcagaataat gcagaatcgc caacaggtta ctggcgaggt tatcgagaaa
     5281 cgccagactg atacccataa aaaagagaat aaggtaaacc atccagctta atagctgatt
     5341 aataactttt aacgcagctt gttggcgaag cggaatgagg taacccacaa tcaggggaac
     5401 cagaatgatt aacagcccag aaaacatgaa aacccagtcc ttgcaaagat gaagtcgaaa
     5461 tgcgcgatga cacactactg aaagcggaag gacgagtaaa gttgcaatta aaaggaaatg
     5521 ttatgcataa ggagcagtag agtattcgtt ttcatttaaa gatattcttg cgctttaatt
     5581 acaaactgca ccgatgttgg tggcgtcaaa atcgccgagg cgttccctga aggccggggc
     5641 agcccacatg gatgtgggct gagggcgcgt tttacaggga tgttacctcg cgcccgaccc
     5701 ggtagccgta agggataagt cgagggcacc gcgcagcggc gattttgttc gccagagccc
     5761 gggggtgcag ggggcggcgg cgattggccg ccccctgcgc gctctttgcg ccagtggcaa
     5821 tatgtggctt agctcatgaa aggagcgcaa caagatgatg aatcaacata taacaacatc
     5881 ttaaaaaaag gcctgacatt acgccaggcc ttctgcgtta attaatcacg cttttccagc
     5941 agggtccggt aaatcagacc accgataatg ccgccgacaa ttggcaccac ccagaagaac
     6001 cacagttgtt ctaatgccca gccgccctgg aagatagcaa ccgcggtgct gcgcgccggg
     6061 ttaacagaag tgttagtcac cggaatacta attaagtgaa tcagggttaa ggccagacca
     6121 atagcgatcg gcgcaaaacc tgccggcgcg aatttgtcgg ttgtgccgtg gatcaccaac
     6181 aggaaacctg cactcaatac cagttcaact accagcgcgg aaagcatgga ataaccgcct
     6241 ggtgaatgct cgccataacc gttagaagca aaaccgctgg ctgccgcgtc aa
//
LOCUS       DAMZET010000210         6188 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.210, whole genome shotgun sequence.
ACCESSION   DAMZET010000210 DAMZET010000000
VERSION     DAMZET010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.210"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            177..563
                     /locus_tag="POB34_004262"
     CDS             177..563
                     /locus_tag="POB34_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDI5921113.1"
                     /translation="MSDPISGTGLAGGALTGASIYGLLTGTDYGVVFGAFAGAVFYIA
                     TAADLSASRRLAYFIVSYIAGILCSGLVGSKLANLTGYSDKPLDAIGAVIVSALAVKI
                     LTFLNNQDIGSLVALITRRGGSGGAK"
     gene            550..831
                     /locus_tag="POB34_004263"
     CDS             550..831
                     /locus_tag="POB34_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDI5921114.1"
                     /translation="MELNDPTATINALLCACVVITLMFYRRGDSRHRPWISRLAWLLT
                     VIYSAVPLAYLCGIYPYSSWATIVANIIFLSVLVAVRGNVARLVDHLRH"
     gene            831..1445
                     /locus_tag="POB34_004264"
     CDS             831..1445
                     /locus_tag="POB34_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HDI5921115.1"
                     /translation="MNQSQFQQAAGISADVSARWFPHIDAAMKEFGITAVNDQAMFIA
                     QVGHESAGFTSLAESFNYSVEGLKKTFGKRLTPYQCEMLGRVDGKQVAHQPQIANLVY
                     GDRMGNNSQGDGWKYRGRGLLQITGRENYTKCGAALKLDLVSTPELLTQERHAARSAA
                     WYFTLRGCLMYSGDVERVTQIINGGQNGIKDRRERYAKAKAALV"
     gene            1453..1722
                     /locus_tag="POB34_004265"
     CDS             1453..1722
                     /locus_tag="POB34_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003833968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921116.1"
                     /translation="MGLEMIIGLVVAVLAAIAGAFGLGKSRGTSIAETKADQQRTEER
                     AAATEAVAERRVETTKGAWDVQQTVNHLPDDDVDRELRENFTRKT"
     gene            complement(1904..2293)
                     /locus_tag="POB34_004266"
     CDS             complement(1904..2293)
                     /locus_tag="POB34_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921117.1"
                     /translation="MTNNYMNDFLKYSQPYVPKDYNLANEFHRRLICWINDFHRELEE
                     EYEVGGQLASFGKNIEFHFTDISYWNPSLISFIGTLEDGSPVELVQHVSQINVLLIRK
                     KRLCLETPKRPIGFADWGEYDSFKGDN"
     gene            2392..2529
                     /locus_tag="POB34_004267"
                     /pseudo
     CDS             2392..2529
                     /locus_tag="POB34_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135225.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            2647..3057
                     /locus_tag="POB34_004268"
     CDS             2647..3057
                     /locus_tag="POB34_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDI5921118.1"
                     /translation="MNASYAVIENGMVVNVIVWDGEDEFTVPDNLQLINISDISEQPG
                     IGWAYSDGIFTAPLPPERSHDELVADAEQKKQSLIDAAMANISVIQLKLQAGRKLTQE
                     ETTRLNVVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(3029..3469)
                     /locus_tag="POB34_004269"
     CDS             complement(3029..3469)
                     /locus_tag="POB34_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDI5921119.1"
                     /translation="MNYIYSATTNSFYPLEMKEDYTQADSWPDDAVEVDEQAYIEFSG
                     LPPKGKIRIAGENGFPAWSEIPPPTHEEQIAAAELEKQQLINQANDYMNSKQWAGKAA
                     IGRLKGEELAQYNLWLDYLDALELVDTSSAPDIEWPTPPAVQAR"
     gene            complement(3491..3880)
                     /locus_tag="POB34_004270"
     CDS             complement(3491..3880)
                     /locus_tag="POB34_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001568588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921120.1"
                     /translation="MPFACYFCIFINVGLGEAAKRNVGTGTNQIPDMGSFTLSVSGTG
                     YQKLPSGFILQWGSIGAPGIAQDVVTHFPIAFPNRCLRVLVSQDYTPDSGSVGYIACA
                     AFSSDPVKFISRASTPGLGASFLALGC"
     gene            3910..4032
                     /locus_tag="POB34_004271"
                     /pseudo
     CDS             3910..4032
                     /locus_tag="POB34_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(4134..6023)
                     /locus_tag="POB34_004272"
     CDS             complement(4134..6023)
                     /locus_tag="POB34_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDI5921121.1"
                     /translation="MKSETLTVQQLFQDRRQYCVPFYQRAYVWTQQDQWSALLEDILE
                     KAQSRLSGTKPTPHFLGAVVLEPQSKKGLLGVDSIHIIDGQQRLTTLQYVLASIRLAL
                     RATDLFSLEALISPCLKNSNEDTMRNKEVERFKLWPTFRDQTHFIQSFNVENIDDLRD
                     VFSDSFTQHGTLRKHFNHPPSLEALCFFTEAFIKWIKIENHSPQENAVALIEAVLTDL
                     KLVSIFLEAEDDAQIIFETLNGRGAELHATDLIRNYIFMCAEHENINAIELYENEWKS
                     FEDKYWSEKQRRGRINKPRMEWLVHATLQSERQREIDLSRLYNEYRDYVSKDLSSQRA
                     DLQVKRLKQYASQYKELVDGFGTTPISHFGYRIADYDVTTLYPLALFISIANIADDEK
                     AAMYNDLVSYVVRRAVCGLTPKNYNNVFMNVLRHLSKTEISSVELRNILNSLNGEASR
                     WPGDSEFLNACINAPLYPGRLDAPKMRSMLTELERELCRQVKTEKPDGPNLSNLDIDH
                     LMPQSWFSCWPLENGHMVTNSDATVLNQIVLSGTDLTPEQLLVRKRQQAIATLGNLTL
                     LNLSVNRSVQNAVFLKKRDALIVHTNLRLNIPLIVKDKWDEDEILERGKKLGEIALKV
                     WPKHD"
BASE COUNT         1663 a         1328 c         1444 g         1753 t
ORIGIN      
        1 tattgtattt tattagctcc atgttgcttt ttgcattaga aatgttaaat agcatcatta
       61 agaaatagtt aatgaattag ttataattac atcaattcta tggctgccaa atggtggcct
      121 tttttattcc tctcatttct atttctgaga ggactcactg caataagagg tggctaatgt
      181 ccgatccgat ttccggcaca gggttagctg gtggagccct aacgggcgct agtatttatg
      241 gactgctgac cggaactgat tacggcgttg tatttggcgc atttgcaggg gctgtattct
      301 acatagcaac agcagcagat ctgagtgcat cgcgccgact agcatatttt atcgtgtcat
      361 atattgccgg gatcctttgc tctgggttgg ttggctccaa gctggcgaac ttgaccggat
      421 acagtgataa acctctggat gctattggtg ccgtaatcgt ctctgcttta gccgttaaaa
      481 tcctgacgtt cctgaataat caggatatcg gctcgctggt ggcgctcata acgcgccggg
      541 gaggttcagg tggagctaaa tgacccgaca gcaactataa atgcgctgtt atgtgcttgt
      601 gttgttatta ctctgatgtt ttatcgtcgt ggcgactcca gacatcgacc gtggatatct
      661 cgcttagcgt ggctgcttac ggtcatttat agcgccgtac cgctggcgta tctgtgcggt
      721 atctaccctt attcatcgtg ggccactatc gtggccaaca ttattttcct gtccgtgctg
      781 gtcgccgtca gaggcaatgt ggcacgtctg gttgaccatc tgaggcacta atgaaccaat
      841 cacaatttca gcaggcggct ggtatcagcg ccgatgtttc tgcacgctgg tttccgcaca
      901 ttgatgcggc aatgaaagag tttggtatta cagcagttaa tgatcaggcc atgttcattg
      961 cacaagtcgg gcatgagtct gcaggattta cttcgctggc agagagtttt aactactcgg
     1021 ttgaagggct gaagaaaacc tttggtaagc gtctgacgcc gtaccagtgc gaaatgctgg
     1081 ggcgtgtcga tggtaaacag gtcgctcacc agccgcaaat agccaatctg gtttacggtg
     1141 accgcatggg gaataacagc cagggtgacg gctggaaata tcgcggtcgt ggcctgcttc
     1201 aaattactgg ccgtgagaac tacaccaaat gtggtgcggc gctgaaactt gaccttgtca
     1261 gtacaccaga attgctaacg caggagcgac atgcggcccg ttcggcggcg tggtacttca
     1321 cgttacgcgg ttgcttgatg tattcgggtg acgtggaacg tgtaacgcag attattaacg
     1381 gcggacagaa tggcattaaa gaccgccgtg aacgttacgc caaagcaaaa gccgcactgg
     1441 tatgaggtca ctatgggact tgaaatgatt atcggcctgg ttgttgctgt gctggctgca
     1501 attgcaggtg cttttggtct gggtaaatca cgcggtacaa gtatcgcaga aacaaaagcg
     1561 gaccagcaac gcactgaaga acgtgcagct gctactgaag ccgttgcaga acgtagggta
     1621 gagacaacaa aaggagcctg ggatgtacag cagactgtta atcatctacc tgatgacgat
     1681 gttgaccgtg agttgcgcga aaactttacc cgcaaaacct gaagtaacgg acacggcctg
     1741 tgactgggta agaatcatct acctcacagg tcaggacatt gaagtgctgg ataagcttac
     1801 gaagcgggat attctggcac ataacaaagc agtacagcgc aactgtatta gccagttatg
     1861 aggcaaacga gattgcgggc aattatcgcc cgcaataaag ttatcaatta tctcctttaa
     1921 aggagtcgta ttcaccccaa tcagcaaacc caatcggtct tttaggggtc tctaagcata
     1981 agcgtttttt tcggattagc aggacattta tttgagatac atgctgaaca agttctacag
     2041 gactgccatc ctccagagta ccaatgaaag aaattaatga tggattccag tagcttatgt
     2101 ctgtgaaatg aaattcaatg tttttaccaa agctagctag ttgtcctcct acctcatact
     2161 cttcttcaag ttcacggtga aaatcattta tccagcatat aaggcgtcta tgaaattcat
     2221 ttgcaagatt gtagtccttt ggaacatatg gctgtgagta tttgaggaaa tcattcatgt
     2281 agttatttgt catcagggcg tcctttacga gcataaccat tgttacttaa cggattgaaa
     2341 cgctattaaa taacatggtt tctatcataa tttctatccg gagttttcca tatgccgcca
     2401 cgaatcccaa aagcctgccg tgttcgcggc tgtcgttcta ccactacaga tccgtcaggc
     2461 tactgcgaaa atcataaagg tgagggctgg aaatcctaca agccaggtca atcacggcag
     2521 cagcgcggat agaggcaggg catttggttc aggcggaggt ggtgcatacg atgcaggtta
     2581 tagcggaacc agtatgacgg gcgggaaagg tgctgctggg gtttgtatta tcgaggagtt
     2641 tgcataatga atgcgtcata tgcagttatt gaaaatggga tggttgtgaa tgtcattgtc
     2701 tgggatggcg aggatgaatt cactgtgccg gataatctgc agctcattaa tatttctgat
     2761 atcagtgagc agcccggaat cggctgggcg tattcagacg ggatatttac tgcgccgctc
     2821 cctccggaac gttctcatga tgaactggta gctgacgctg aacagaaaaa acagtcgctg
     2881 atagacgcag caatggccaa tatcagcgtg attcagttaa agctgcaggc cgggcgcaaa
     2941 ctgacgcaag aagaaactac cagacttaac gttgtgctgg attacattga cgcggtgaca
     3001 gcaactgata ccagcaccgc gccagatgtc atctggcctg aactgccgga ggcgtaggcc
     3061 attcaatatc tggcgcactg gaagtatcga ccagctccag tgcgtccaga taatccagcc
     3121 acaaattata ttgcgccagt tcctcacctt tcagacgacc aatagccgct ttaccagccc
     3181 attgtttact gttcatataa tcgttggcct gattaatcaa ttgctgcttt tccagttcgg
     3241 ctgcagcaat ctgttcctca tgtgttggtg gtggaatttc agaccatgca ggaaaaccat
     3301 tttctccagc gatacggatt tttcctttcg gcggtaatcc ggaaaactca atatacgctt
     3361 gctcatcaac ttcaacagca tcatctggcc atgagtcagc ttgagtgtaa tcctctttca
     3421 tttccagtgg atagaaagag tttgtagtcg cggaatatat gtaattcatt tttcactcca
     3481 tatagctaaa ttaacagcct aacgctaaaa atgaagcgcc gagacctgga gtactggctc
     3541 tggatataaa tttaaccggg tcggaactaa aagctgcgca ggcaatataa ccaacagacc
     3601 cgctatctgg tgtgtagtct tgtgagacca aaacacgcag acatcggttt ggaaatgcaa
     3661 tcggaaaatg ggttaccaca tcctgtgcaa tgcctggtgc gccgattgag ccccactgaa
     3721 gaataaaacc agatggtaat ttttgatatc cagtacctga aacagaaagc gtgaagctac
     3781 ccatatcagg tatctgattc gtccctgtcc ccacattcct ttttgccgct tctcccaaac
     3841 caacgtttat gaaaatgcag aaataacaag caaatggcat cattcctgct tttgtcaggg
     3901 agatctatca tgcttattgg ttatgtacgc gtatcaacaa atgaccagaa taccgatcta
     3961 caacgcaatg cgctgaactg tgcagggtgt gagctgattt ttgaagacaa gataagcggt
     4021 acaaagtccg agacctaggg ctgacactgc tgttcttgat gcaaattaaa ggatttaata
     4081 gtgagtagcg gccttactaa tgtaaggccg ctataatcat tttattaatt gcattaatcg
     4141 tgttttggcc atactttcaa tgcaatttcc cccaactttt taccccgctc caggatttca
     4201 tcctcatccc atttatcctt aactataagt ggaatgttca gtcgtagatt ggtgtggacg
     4261 atgagagcat cacgtttttt cagaaataca gcattctgaa cagaacggtt tacgctaagg
     4321 ttaagcaaag ttagatttcc caacgtagct atcgcttgtt gccgtttcct taccagtagc
     4381 tgttcagggg taagatcggt tccagacaga acaatttggt tcaataccgt agcatctgaa
     4441 tttgtcacca tatgaccatt ttcgagaggc caacaggaaa accaactttg aggcataaga
     4501 tgatcgatat cgagattgga aagatttgga ccatcaggct tctctgtctt cacttggcga
     4561 caaagttctc tttcaagttc cgttaacatt gagcgcattt tcggtgcgtc gagcctgcca
     4621 ggataaagtg gagcattgat gcaagcattg agaaattctg agtcaccagg ccaacgtgag
     4681 gcttcgccat ttaagctatt gaggatatta cgtaactcaa cactggaaat ttccgtttta
     4741 gacaagtgcc gcaatacatt cataaataca ttgttgtaat tctttggcgt caggccacat
     4801 acggctcttc gtactacgta ggagacaaga tcattataca tggctgcttt ctcatcatca
     4861 gcgatgttag ctatcgaaat gaacaaagca agcggataaa gtgtcgtcac atcatagtct
     4921 gcgatgcgat atccaaagtg tgagatgggg gttgtgccaa aaccatcaac caattcttta
     4981 tattgtgatg catattgttt gaggcgcttt acttgcagat ctgctcgttg tgaagacaag
     5041 tccttactta cataatcacg atactcattg taaaggcgag acagatcaat ttcacgctgc
     5101 ctttctgatt gcaatgtcgc atgtactagc cactccatgc gtggtttatt aatacgtcca
     5161 cggcgttgct tttccgacca gtatttatct tcaaagctct tccactcatt ttcatataat
     5221 tcaatagcat taatattttc atgctcagcg cacataaaga tatagttgcg aataagatcc
     5281 gtggcatgaa gttccgcccc tcgcccattt aatgtttcaa aaattatttg ggcatcatct
     5341 tcagcttcga gaaatatgct taccagtttc agatctgtta agacagcctc aattagagct
     5401 acagcatttt cttgtggtga gtggttttct attttaatcc attttataaa ggcttcagta
     5461 aaaaaacata atgcttctag tgatggcggg tgattaaaat gcttacgcaa cgtaccatgc
     5521 tgcgtgaaac tatcagagaa tacgtcccgg agatcgtcaa tattttcaac attaaaactt
     5581 tgaataaaat gagtttgatc ccgaaaagtt ggccatagtt tgaagcgttc tacttcttta
     5641 tttcgcattg tgtcttcgtt tgagtttttc aggcaaggcg aaatgagagc ctctaagctg
     5701 aaaagatccg tagcacgtaa tgctaatcgg atggatgcca gaacatattg aagagtggtc
     5761 aatcgttgct gaccatcaat aatatgtatg gaatctacac ctaataatcc tttttttgat
     5821 tgaggttcca gtacaaccgc accaaggaaa tggggagttg gttttgtacc cgagagccgg
     5881 gattgtgctt tctctaggat atcctccagt agagctgacc attggtcttg ctgagtccat
     5941 acataggcac gttgatagaa tggaacacag tattgtcggc ggtcttgaaa aagttgttgg
     6001 acagttagcg tctcggattt catggtatac ctgtaagcga cattgtacaa aagtgaggag
     6061 ttaatctgat ttagaacaat cattttattt tgctatgata caacgtattt gttaccggtt
     6121 aagcaaaaaa cgcgtctgaa cgctgacgtt ttagggcttt actggttatt ctttcttatc
     6181 tcttcatc
//
LOCUS       DAMZET010000211         6169 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.211, whole genome shotgun sequence.
ACCESSION   DAMZET010000211 DAMZET010000000
VERSION     DAMZET010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.211"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..569
                     /gene="manC"
                     /locus_tag="POB34_004273"
     CDS             <1..569
                     /gene="manC"
                     /locus_tag="POB34_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase ManC"
                     /protein_id="HDI5921122.1"
                     /translation="THTAEGNVCRGDVINYKSENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRSSVQDVKHVVEQLKADGRHEYRMHREVYRPWGQYDSIDAGARYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTINGDIKLLGENESIYIPLGATHCLENPGKI
                     PLEIIEVRSGSYLEEDDIVKFYDRYGRD"
     gene            639..2027
                     /gene="cpsG"
                     /locus_tag="POB34_004274"
                     /gene_synonym="manB"
     CDS             639..2027
                     /gene="cpsG"
                     /locus_tag="POB34_004274"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001700823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HDI5921123.1"
                     /translation="MTRLSCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKRALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAVFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFANWRFNLRSSNTEPVVRLNVESRGDLGLVEKQTTYI
                     INLLTTPPNDEK"
     gene            2014..2973
                     /locus_tag="POB34_004275"
     CDS             2014..2973
                     /locus_tag="POB34_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="wavE lipopolysaccharide synthesis family
                     protein"
                     /protein_id="HDI5921124.1"
                     /translation="MMKSENITFVVQGPVRNETKDTLESIRLNFKEAKIILSTWEGSN
                     VKGLSFDDIVFSNDPGPLNIKRGTKIVAQENTNRQIISTYQGLIKVRTPYAVKLRTDT
                     PLRNDNIIKKYIKAQAYGRDYNFSYLEERILVSSINTIDPKSYIQFPYHISDWIYFGK
                     TNDLLKIWDGELIDDNDYFTEQDIKEEISCKRGFEFGRYTAEQLVLYSFLKKNKISEY
                     KHYCDTNNDRVEKVLKVILSNFYCVSPKQMGLCFDKYSDLITPRMNYRSLRSFLGFYF
                     VTINERVWRNYYSQFYKLDTTISEKIEICARRIFIKIMKKRKK"
     gene            2970..4091
                     /locus_tag="POB34_004276"
     CDS             2970..4091
                     /locus_tag="POB34_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000045904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDI5921125.1"
                     /translation="MSKSMLYIYTHNGYNDAFLKKDVGMVPSVMLNGGKVDSLIYCKE
                     KKGKAVFNNLEIYFTGCGWFISNFKAILFLCRNAIFNRHLDLLIFHLSLRNIVPVLTY
                     KLLFKGELICKMDLNTESAIEYSDKSHKINGIKKKLIRMIISRTDQFYVETIRNYNII
                     NKGIFDVPISGKLKLMPNGVDAKLVREVIKTEGPDVFKENTITIISRHACEAKAPNRI
                     FDVIDVMSELKLNNWTLQILGYFPTIFTDALRIRALECGVNIICDGYNLELYDVYKRL
                     FKSKMFICLSLQESYCISLVEAAVFNNHIISTNVGVAEDLSLKYGNIKIIENYSKNLL
                     SKTIEKTIKNDIKIKKASISDRMLDSYSWDNIITGVMSE"
     gene            4093..5214
                     /gene="wzy"
                     /locus_tag="POB34_004277"
     CDS             4093..5214
                     /gene="wzy"
                     /locus_tag="POB34_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039025712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O78 family O-antigen polymerase"
                     /protein_id="HDI5921126.1"
                     /translation="MGVYFILMTVALLISIIVLFHNDVKFTNVYIYTYILLLSLLAGL
                     RFHTGWDWEAYDYFFYELTSSNLFEISKANVFNYEPGFVLLSYISVLLHIPPFLFFSL
                     ITVTLIIKSAYKYLGNFVYIFLLIYLYYGYFHNFSIVRQGVAAALFAYSIRYLIAKSY
                     KYYILIFVAALFHMSAIMLLIVPFLYKIANRIPFSALLLFSLLMVYTSVSELIGMKSL
                     LSGINALNIYINNSSLSYKVGFSFKYLELLIILVLFYNKRFSELIASKFSKKQYYVFR
                     CLIVIEVMIYSLFNDFSIIYERLTVYFEFSHAICIAMIISAFKYKRVQLFLLLILLSL
                     IFVRYYQLFNSPVRVDGEMTHYERFENYCSVFNTKDCQR"
     gene            5217..5594
                     /locus_tag="POB34_004278"
     CDS             5217..5594
                     /locus_tag="POB34_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HDI5921127.1"
                     /translation="MKRIIIDLDNTITIHNDEPYGKKPVNQDVVNKMKMYKELGYTIT
                     IFTARNMNTFSGNIGKINVHTMPVIIDWLNRNNIPYDEIIVGKPWCGTNGFYVDDKAI
                     RPSEFISLSETEIAELLDKEVKK"
     gene            5591..>6169
                     /locus_tag="POB34_004279"
     CDS             5591..>6169
                     /locus_tag="POB34_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921128.1"
                     /translation="MILINSAAYVNDDLRAEFGLLPPCFLPLGNRRLYHHQIESLRKA
                     FPEEKIYLSLPEEYHIGRYDQQYFNENDITILKTSYTLSQGHSLYRLLNDFNLIECDK
                     LIINYGDTFYHTFEATSDDYFYVSNNIGYYNRASVVINGDKLIINKDNPAVDNGELVI
                     SGFFCFSNIGRLCECLKKMNYDFLSSSELYYSS"
BASE COUNT         1981 a         1013 c         1247 g         1928 t
ORIGIN      
        1 gcactcacac agctgaaggc aacgtttgcc gcggtgacgt tattaattat aaatccgaaa
       61 acagttatgt gtatgccgaa tcgggcctgg tcaccaccgt cggggtgaaa gatttggtgg
      121 tagtgcagac caaagatgcg gtgttaatag ccgatcgcag ctcggttcaa gatgtgaaac
      181 atgtggttga gcagctcaaa gccgacggtc gacacgagta tcggatgcat cgcgaagtat
      241 accgtccgtg ggggcaatat gactctatcg atgcaggcgc tcgctaccag gtgaaacgca
      301 taactgtgaa accgggcgag ggcttgtcgg tacagatgca tcatcaccgc gcagaacact
      361 gggtggtggt tgcgggaacg gcaaaagtca ctattaatgg tgacatcaaa ctgcttggtg
      421 aaaatgaatc catctatatt ccactggggg cgacacattg tttggaaaat ccaggaaaaa
      481 tacctttaga aataattgag gttcgctcag gttcttatct tgaagaagat gatatagtta
      541 agttttatga tcgttatggg cgagattaat ttcattactg aagttgctaa ttgtttaaca
      601 aaatacttct gtgtattttt tcatataagt ttgtaaatat gactagatta agctgcttta
      661 aagcctatga tattcgcggg aaattaggcg aagaactgaa tgaagatatc gcctggcgca
      721 ttggtcgcgc ctatggcgaa tttcttaaac cgaaaaccat tgttttaggc ggtgatgtcc
      781 gcctcaccag cgaaacctta aaacgggcgc tggcgaaagg tttacaggat gcgggcgtcg
      841 acgtgctgga tatcggtatg tccggcacag aagagatcta tttcgccacg ttccatctcg
      901 gcgtggatgg cggcattgaa gttaccgcca gccataaccc gatggattat aacggcatga
      961 agctggtgcg tgagggggcc cgcccgatca gcggcgatac cggactgcgc gatgtccagc
     1021 gtctggcaga agccaacgac tttccccccg tcgatgaaac caaacgcggt cgctatcagc
     1081 aaatcaacct gcgtgatgca tacgttgatc acctgttcgg ttatatcaat gtcaaaaacc
     1141 ttactccgct gaaacttgtg atcaactccg gaaacggcgc agcaggtccg gtggtggacg
     1201 ccatcgaagc ccgctttaaa gccctcggcg caccggtgga attaatcaaa gtgcacaaca
     1261 cgccggacgg caatttcccc aacggtattc ctaacccgct gctgccggaa tgccgcgacg
     1321 acacccgcaa tgcggtcatc aaacacggcg cggatatggg cattgccttt gatggcgatt
     1381 ttgaccgctg ttttctgttt gacgaaaaag ggcagtttat cgagggctac tacattgtcg
     1441 gcctgctggc agaagcgttc ctcgaaaaaa atcccggcgc gaagatcatc cacgatccac
     1501 gtctctcctg gaacaccgtt gatgtggtga ctgccgcagg cggcaccccg gtaatgtcga
     1561 aaaccggaca cgcctttatt aaagaacgta tgcgcaagga agacgccatc tacggtggcg
     1621 aaatgagcgc ccaccattac ttccgtgatt tcgcttactg cgacagcggc atgatcccgt
     1681 ggctgctggt cgccgaactg gtgtgcctga aaggaaaaac gctgggcgaa ctggtgcgcg
     1741 accggatggc ggtgttcccg gcaagcggtg agatcaacag caaactggcg caacccgttg
     1801 aggcgattaa ccgtgtggaa cagcatttta gccgtgaggc gctggcggtg gatcgcaccg
     1861 atggcatcag catgaccttt gccaactggc gctttaacct gcgctcctcc aacaccgaac
     1921 cggtggtgcg gttgaatgta gaatctcgtg gtgatttagg acttgttgaa aagcagacaa
     1981 catatatcat taacttatta acaacccccc ccaatgatga aaagtgaaaa tattacattc
     2041 gtagtgcaag gtccagtcag aaatgaaaca aaagatactt tagaaagtat taggctaaat
     2101 tttaaagaag ctaaaataat tttgtccaca tgggagggga gtaacgttaa aggtctttca
     2161 tttgacgata ttgtttttag taacgatcct ggccctttaa atataaaaag aggtactaaa
     2221 atagttgctc aggaaaatac aaatcgtcaa ataatttcaa cataccaagg tttaattaag
     2281 gttcgaacac catatgcggt taagttgaga acagatacgc cattgcgaaa tgataatatt
     2341 ataaaaaaat atataaaagc tcaagcatat gggagagatt ataatttttc atacttagag
     2401 gaaaggattc ttgtatcatc tataaatact atagatccaa aaagctatat tcaattccca
     2461 taccatattt ctgattggat ctactttggt aaaactaacg atttattaaa aatttgggat
     2521 ggggaattaa tagatgataa tgattatttt actgagcagg atataaaaga agagattagt
     2581 tgcaaaagag gatttgagtt tggaagatac acagctgaac agcttgttct atactcattt
     2641 ttaaaaaaaa ataaaattag cgaatataaa cattattgtg atacaaataa tgacagggtt
     2701 gaaaaggtat taaaagtaat cctttctaat ttctattgcg taagtcctaa acaaatggga
     2761 ctctgttttg ataaatactc agacttaata acccctagaa tgaattacag gagtctccgt
     2821 agtttcttag ggttttattt tgttacaatt aatgagagag tttggcgaaa ttattacagc
     2881 caattctata aacttgacac tacaatatca gaaaaaatag aaatatgtgc acgcaggata
     2941 tttataaaga tcatgaagaa gaggaaaaaa tgagtaaaag catgttatat atctacacac
     3001 ataatggtta caatgatgca tttttaaaaa aagatgtagg gatggtacca tctgttatgt
     3061 taaatggtgg gaaagttgat tcgctaattt attgtaaaga aaagaaagga aaggctgttt
     3121 ttaataattt agaaatatat tttactggat gtggatggtt tattagtaac ttcaaggcga
     3181 tactgtttct ttgtcgaaat gcaatattta atagacattt agatttattg atatttcatc
     3241 tcagccttag aaatatcgtg cctgtgttaa catataaact tttatttaaa ggggaattaa
     3301 tatgtaagat ggatttaaat actgaaagtg ccatcgaata ttctgataaa agccataaaa
     3361 taaatgggat caaaaaaaaa ctaataagaa tgattatttc ccgcacagat caattttatg
     3421 ttgagactat aagaaattat aacataataa ataaaggcat ttttgatgtg ccgatttcag
     3481 gaaagttaaa attaatgccc aacggagttg atgctaaatt agttagggag gttattaaaa
     3541 ctgaaggtcc tgatgtattt aaagaaaata caataacaat aatctctcgc catgcttgtg
     3601 aagcaaaggc tccaaacaga atattcgatg taattgacgt aatgtctgaa ttgaaactta
     3661 ataactggac attgcaaata ttgggctatt tcccaacaat atttacggat gctctaagaa
     3721 taagagctct tgaatgtgga gtgaatatca tatgtgacgg ttataattta gagctctatg
     3781 atgtgtataa aagattattt aaaagtaaga tgtttatatg tctttcgctg caagagtcat
     3841 attgtatatc actagttgaa gctgctgtat ttaataatca tataatatct acaaatgttg
     3901 gagtagcaga agatctttca ttaaaatacg gtaatattaa aattatcgaa aattattcga
     3961 aaaatttact atccaaaact attgaaaaga caattaaaaa tgatattaaa ataaaaaaag
     4021 caagcatttc tgatagaatg ttagactcat attcttggga taatatcata acaggggtca
     4081 tgagcgaata atatgggagt atatttcatt ttaatgacag tggcactatt gatctctatt
     4141 atcgtattat tccacaatga tgttaaattt acgaatgtat atatatatac ttatatttta
     4201 ctgctctcat tacttgctgg tttaagattt cataccggct gggattggga agcttatgac
     4261 tatttttttt atgagctgac aagttctaat ttatttgaaa taagcaaagc aaatgttttt
     4321 aattatgaac cagggttcgt tttactatct tatatatctg ttttattgca cataccacca
     4381 tttctctttt tctctttaat aaccgttaca ttaattataa agtcggctta taagtattta
     4441 gggaactttg tttacatttt tttattaata tatttatatt acggttattt tcataatttt
     4501 tctattgtac gacaaggtgt tgctgctgcg ttattcgctt attccattcg ttatttgata
     4561 gcgaaaagct ataaatatta tatattaata tttgtcgctg cattattcca tatgtctgct
     4621 attatgttat taattgtccc atttctttat aaaatcgcta atcgtatacc attttccgcc
     4681 ctattgctat tttcattgtt aatggtttat acctctgtaa gtgagctcat aggtatgaaa
     4741 tcattattgt caggaataaa tgcactaaat atatatatca ataatagttc tttaagctat
     4801 aaagttggtt tttcgtttaa atatttggag ttattaatca ttctagttct attttataat
     4861 aagagatttt cagaactgat tgctagcaaa tttagtaaaa agcaatatta tgtatttaga
     4921 tgtttgattg taattgaagt gatgatatat tcgcttttca atgattttag cattatttat
     4981 gaaaggctaa ctgtttactt cgaattttct catgctatat gtatagcaat gattatatca
     5041 gcctttaagt ataaaagagt tcagttgttt ttactgttaa ttttattgtc tcttattttt
     5101 gtaagatatt atcaattatt caattctcct gttcgtgtcg atggagaaat gacacattac
     5161 gaacgatttg aaaactattg ttctgttttt aacacaaaag attgtcaaag gtaacaatga
     5221 agcgaattat tatagacttg gataatacta tcacaatcca taacgatgaa ccatacggta
     5281 aaaaaccagt aaatcaagat gttgtaaata aaatgaagat gtataaagag ctgggttata
     5341 caatcacaat atttaccgcc cgtaatatga atactttttc aggtaacatt gggaagataa
     5401 atgttcatac aatgcctgtt ataattgact ggttaaatcg taataatatt ccatatgatg
     5461 aaattattgt gggtaaacca tggtgtggga caaatggatt ttatgttgat gataaggcga
     5521 ttaggccttc agagtttata agcttgtcag aaacggagat tgctgaacta ctggataagg
     5581 aggtaaaaaa atgattctaa taaactctgc tgcttacgtt aatgacgacc taagagctga
     5641 attcggtttg ctaccaccgt gctttttacc ccttggtaat agaagactat atcatcatca
     5701 gattgagtcg ttaaggaaag cttttcctga agaaaaaata tatttatctc tgccggaaga
     5761 gtatcacatt ggtaggtatg atcaacaata ctttaatgaa aatgatatta caatactaaa
     5821 aacttcttat actttatcac aaggacatag tctgtatcgg ttgttaaatg attttaactt
     5881 aatcgaatgt gataaactaa ttattaatta tggtgatact ttttatcata cctttgaagc
     5941 tacgagtgat gattattttt atgtatctaa taacatcggc tactataacc gagcatctgt
     6001 tgtaattaat ggtgataagc ttattataaa taaagataat ccagctgttg ataatggtga
     6061 gttggttatt tctggtttct tttgtttcag taatattggg aggttatgcg aatgcttgaa
     6121 aaagatgaac tatgattttc tgtcgtcttc tgaattgtat tactcgtcg
//
LOCUS       DAMZET010000212         6145 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.212, whole genome shotgun sequence.
ACCESSION   DAMZET010000212 DAMZET010000000
VERSION     DAMZET010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.212"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..656
                     /locus_tag="POB34_004280"
     CDS             <1..656
                     /locus_tag="POB34_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921129.1"
                     /translation="STSLWYLYALIVYFVVCKIFSRLALPLFALFVLLSVAVNFVPTP
                     WWGMNSVIRNLLYYSLGAWFGATIMTCVKEVPLRRHLLMASLLTVLAVGAWLFTISLL
                     LSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSNTIAIYTTHRILVEIFSLTLLAQMN
                     AARWSPQVELTLLLVYPFVSLFICTVAGLLVRKLSQRAFSDLLFSPPSLPAAVSYSR"
     gene            784..4230
                     /gene="mfd"
                     /locus_tag="POB34_004281"
     CDS             784..4230
                     /gene="mfd"
                     /locus_tag="POB34_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDI5921130.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVHEYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARILLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            4374..5336
                     /gene="ldtC"
                     /locus_tag="POB34_004282"
     CDS             4374..5336
                     /gene="ldtC"
                     /locus_tag="POB34_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HDI5921131.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(5419..5676)
                     /gene="bhsA"
                     /locus_tag="POB34_004283"
     CDS             complement(5419..5676)
                     /gene="bhsA"
                     /locus_tag="POB34_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HDI5921132.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            5917..>6145
                     /gene="comR"
                     /locus_tag="POB34_004284"
     CDS             5917..>6145
                     /gene="comR"
                     /locus_tag="POB34_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HDI5921133.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYID"
BASE COUNT         1457 a         1570 c         1631 g         1487 t
ORIGIN      
        1 ccagcaccag cttgtggtat ctgtatgcgt taattgtcta tttcgtggta tgtaaaattt
       61 ttagccgtct ggcgctgcca ctattcgcct tgtttgtact gctgagtgtg gcggttaatt
      121 tcgttcccac gccgtggtgg ggaatgaaca gtgtgatccg caatttgctt tattacagcc
      181 ttggcgcatg gtttggcgca acaataatga cctgtgttaa agaggtgccg ttgcgccgcc
      241 atctgctgat ggcttctttg cttaccgttc tggcggtcgg tgcctggttg tttactatct
      301 cgctgctgtt gtcgctggta tcgattgtgg tgatcatgaa gctgttttat caatacgagc
      361 aacgattcgg tatgcgctcc accagcctgc tgaatgtgat tggctccaac accattgcta
      421 tctacaccac ccatcgcatt ctggttgaaa tattcagctt aactctgctt gcgcaaatga
      481 acgcagcacg ctggtcgccg caagtcgaac tgacactcct gctggtttat ccctttgtca
      541 gtttgttcat ctgtactgtt gcgggcttgc tggtaagaaa actttcacag cgtgcattca
      601 gcgatctgtt gttctccccg ccttcactgc ccgctgccgt cagttactct cgttaaacca
      661 tcgccccagc cggggcgatt caatttgcta aaccatgtcg attacggata atgaacagca
      721 ttgcttatca gagatatgcc cccatatgtt gaggcatatc ctaacgagaa tctgacaacc
      781 gttatgcctg aacaatatcg ttatacgctg cccgtcaaag cgggtgagca gcgtctgctg
      841 ggcgagttaa ccggcgcagc ctgtgcaacg ctggtagcgg aaattgccga acgtcacgcc
      901 ggtccggtgg tactcattgc accagatatg caaaatgctc tgcgtttgca tgatgaaatc
      961 agccagttca ccgatcagat ggtgatgaat ctggcggact gggaaactct tccctacgac
     1021 agtttttcgc ctcatcagga cattatctcc tcgcgccttt ccacccttta ccagctaccg
     1081 acgatgcagc gtggcgtact gattgttccg gtgaatacgc ttatgcagcg cgtttgccca
     1141 cacagttttc tccacggtca tgcgctggtg atgaaaaaag gtcagcgcct gtcacgagat
     1201 gcattacgaa cccaattgga cagcgccggt tatcgccatg ttgaccaggt gatggagcac
     1261 ggcgaatacg ccacgcgcgg cgcgttgctg gatctcttcc cgatggggag tgagctgcct
     1321 tatcgtcttg atttctttga tgatgaaatc gacagcctgc gggtgtttga cgtcgacagc
     1381 cagcgcacgc tggaggaagt agaagcgatc aatctgctgc ccgcgcacga atttccgacc
     1441 gataaagcgg caattgaact gttccgcagc cagtggcgcg ataccttcga agtgaagcgc
     1501 gatccggaac atatttacca gcaagtgagt aaaggcacat tacctgccgg gatcgagtac
     1561 tggcagccat tgttcttcag cgaaccactg ccgccgctgt tcagttattt ccctgccaat
     1621 accttgctgg tgaatactgg cgatctggaa accagtgccg aacgtttcca ggctgacacg
     1681 ctggcgcgtt ttgagaatcg cggcgtcgat ccgatgcgcc cgctgttgcc accacaatcg
     1741 ctctggctgc gggtggacga gctcttctca gagctgaaaa actggccccg ggtgcagcta
     1801 aaaactgaac atttaccgac aaaagccgcg aatgccaatt taggtttcca gaaactgcca
     1861 gacctggccg ttcaggcaca acaaaaagcg ccgctggatg cgctgcgtaa gttcctcgag
     1921 actttcgacg gtccggtggt gttctcggta gaaagtgaag gtcgccgtga agcgctgggt
     1981 gaactgctcg cacgaattaa aattgctccg caacgcatta tgcgtcttga tgaagccagc
     2041 gaccgtgggc gttatctgat gattggcgct gccgaacatg gttttgtcga taaggtgcgt
     2101 aatctggcgc tgatctgcga aagcgatctg ctcggtgaac gtgttgcccg ccgtcgtcag
     2161 gattctcgcc gcaccatcaa ccccgataca ctgatccgta accttgcgga gctgcatatt
     2221 ggtcagccgg tggtccatct ggagcacggt gtcgggcgct atgccggaat gaccacgctc
     2281 gaagcgggcg gcattactgg cgagtatttg atgctcacct atgccaacga cgccaaactg
     2341 tatgttccgg tgtcgtcact gcatctgatt agccgttacg caggtggcgc ggaagaaaac
     2401 gccccgctgc ataaacttgg cggcgatgcg tggtcacgcg cgcggcagaa agcggcggaa
     2461 aaagtgcgtg atgtggcggc ggaattgctg gatatctacg cgcaacgcgc cgccaaagag
     2521 ggcttcgcgt ttaaacacga tcgtgagcag tatcagttgt tctgcgacag cttcccgttt
     2581 gaaaccacgc cggatcaggc gcaggccatt aatgcggtac ttagcgacat gtgtcagccg
     2641 ctggcaatgg atcgtctggt gtgcggcgat gttggctttg gtaaaacaga agtggcgatg
     2701 cgcgccgctt tcctggcagt agataaccac aagcaggtgg cggtgttggt gcctaccacc
     2761 cttctcgcgc agcagcatta cgacaacttc cgcgaccgtt tcgccaactg gccggtacgt
     2821 atcgaaatga tctcccgttt ccgcagcgcc aaagagcaga cgcaaatcct tgcggaagtg
     2881 gcggaaggga aaatcgatat tctgatcggt acgcacaaac tgctgcaaag tgacgtcaag
     2941 tttaaagatt taggcctgct gattgtcgat gaagaacacc gcttcggggt gcgtcataaa
     3001 gagcgtatta aagcgatgcg cgcgaacgtg gatattctga cgcttactgc aacgccgatc
     3061 ccacgtacgc tgaatatggc aatgagcgga atgcgtgacc tgtcgattat cgccacgccg
     3121 cccgcccgtc gtctggcagt taaaaccttt gtccatgagt atgacagcct ggtggtccgg
     3181 gaggcgatcc tgcgtgaaat tttgcgcgga ggacaggttt attatctcta caatgatgtg
     3241 gaaaacattc agaaagccgc cgaacggctg gcagaactgg tgccagaagc gcggatcgcc
     3301 atcggtcacg ggcagatgcg cgagcgcgaa ctggaacggg tgatgaatga tttccatcat
     3361 caacgtttca acgtgctggt ttgtaccacc attatcgaaa ccgggatcga catcccgaca
     3421 gccaacacca ttatcattga acgtgcggat cacttcggtc tggcgcagct gcaccagtta
     3481 cgcggtcgcg tcggacgttc gcatcatcag gcatatgcat ggttgctgac gccgcatcca
     3541 aaagcgatga ctaccgatgc acaaaaacgt cttgaagcga ttgcctcgct ggaagatctc
     3601 ggtgcaggct ttgcgctggc aacgcacgat ctggagattc gcggcgcggg tgaactgctt
     3661 ggcgaagaac aaagcggctc aatggaaacc atcggtttct cgctgtatat ggagttgctg
     3721 gaaaacgccg tcgatgcact gaaagccgga cgcgagccgt cgctggaaga tctcaccagc
     3781 cagcaaacag aagtcgagct gcggatgccg tcgctattgc cagatgattt cattcctgac
     3841 gtgaatacgc gtttgtcgtt ctataaacgt attgccagcg ccaaaacgga aaacgaactg
     3901 gaagagatca aagtcgagct tatcgatcgc ttcggcctgc tgccggatcc ggcgcgtatc
     3961 ctgctggatg ttgcccgtct gcgccagcaa gcgcagaaac tggggatcag gaagctggaa
     4021 ggtaatgaga aaggcggggt gatcgaattt gccgagaaga atcacgttaa tccggcctgg
     4081 ttgattggtt tgctgcaaaa acagccgcag cattaccgcc ttgatggtcc gacgcgcctg
     4141 aaatttattc aggatttgag tgagcggaaa acgcgtatcg aatgggtacg ccagtttatg
     4201 cgtgaactgg aagagaacgc gatcgcttaa tggtaaatcg ggggcgtttc tgcgccccca
     4261 tctgcaacat ttacaaattc tttgcacttc cctgcactat ccgacactgt caccatccat
     4321 aattcaggct tatctgttta ttacaataac cttatattta ttatggattt ttggtgatga
     4381 tcaaaacgcg tttttctcgc tggctaacgt tttttacgtt cgccgctgcc gtggcgctgg
     4441 cgctaccggc aaaagccaac acctggccgc tgccgccagc gggcagtcgt ctggttggcg
     4501 aaaacaaatt tcatgtggtg gaaaatgacg gtggttctct ggaagccatc gccaaaaaat
     4561 ataacgtcgg ctttctcgct ctgttacagg ctaaccccgg cgttgatcct tacgtaccgc
     4621 gcgcgggcag cgtgttaacg atcccgttgc aaaccctact tccagacgcg ccgcgcgaag
     4681 gcattgtgat caacattgcg gagctgcgtc tctattacta cccgccgggt aaaaattcgg
     4741 taaccgtgta tccaataggt attggtcagt taggtggtga cacgctgaca ccgacaatgg
     4801 tgaccaccgt ttcagacaaa cgtgcaaacc caacctggac gccaacggca aacatccgcg
     4861 cccgttataa agcacaggga attgagttgc ctgcggtagt gccggctgga ccggataacc
     4921 caatgggcca tcatgcgatt cgtctggcgg cctatggcgg cgtttatttg cttcacggta
     4981 cgaacgccga tttcggcatt ggcatgcggg taagttctgg ctgtattcgt ctgcgggatg
     5041 acgatatcaa aacactcttt agccaggtca ccccaggcac caaagtgaat atcatcaaca
     5101 ctccgataaa agtctctgcc gaaccaaacg gtgcgcgtct ggttgaagta catcagccgc
     5161 tgtcagagaa gattgatgac gatccgcagc tgctgccaat tacgctgaat agcgcaatgc
     5221 aatcatttaa agatgcagca caaactgacg cagaagtgat gcaacatgtg atggatgtcc
     5281 gttccgggat gccggtggat gtccgccgtc atcaagtgag cccacaaact ctgtaaatcc
     5341 tggataaaaa aaatccccgc gacacggcat gtcgcgggga aaaaatcagc agtagcaggc
     5401 attaatgagg gttaatgctt atttataaat tactgctgtt ccatggaggg tattcggacc
     5461 ggttacagaa gtaatacgga aagattttgc gcccatctca tccgcttttt gcgccagctg
     5521 ctcttccagc gatcccagat ttgtccccgc gttagcactg attgtaccga ctttttgttg
     5581 gccttctggc gttgactgaa cttcgacagc cgcaaagctg gcaaatgaca tggagcttaa
     5641 aatcgccgca gcgatgaggg tttttacgtt tttcataata gtggccttat gcagatgaat
     5701 gacgtcgcaa gcaattactt aacgatcgat aactaaatga tagatgtgat ctggatcaca
     5761 tacaagatat tttttataac aatcattaat taatttaaaa atcattaaat ttcagttaaa
     5821 tagaaagcaa ttatttttat gggatctgga ctggtgaacg gaggtgatta tttttataat
     5881 aacctttcat tcacagtcac caggtacaac ggcatcatgg caactgactc aacacaatgt
     5941 gtaaaaaaaa gccgtggccg cccaaaagtg ttcgacaggg atgccgcgct tgataaggcc
     6001 atgaaattgt tctggcaaca cggttatgaa gcgacttctc ttgcggacct cgtcgaagcg
     6061 accggagcca aagcgcccac gctatacgcg gaatttacca acaaagaggg gttatttcgt
     6121 gccgttctcg accgctatat cgatc
//
LOCUS       DAMZET010000213         6055 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.213, whole genome shotgun sequence.
ACCESSION   DAMZET010000213 DAMZET010000000
VERSION     DAMZET010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.213"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..213
                     /locus_tag="POB34_004285"
     CDS             <1..213
                     /locus_tag="POB34_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HDI5921134.1"
                     /translation="YIGIAFYKVGEPSKIEPDWMINGGVPELKKQLDLNDAVPEISGT
                     ILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            511..552
                     /locus_tag="POB34_004286"
     CDS             511..552
                     /locus_tag="POB34_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921135.1"
                     /translation="MVQLVDLARCVSF"
     gene            590..1972
                     /gene="dosC"
                     /locus_tag="POB34_004287"
     CDS             590..1972
                     /gene="dosC"
                     /locus_tag="POB34_004287"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HDI5921136.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            1997..4396
                     /gene="dosP"
                     /locus_tag="POB34_004288"
                     /gene_synonym="pdeO"
     CDS             1997..4396
                     /gene="dosP"
                     /locus_tag="POB34_004288"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HDI5921137.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKETISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            4654..5235
                     /gene="ddpX"
                     /locus_tag="POB34_004289"
     CDS             4654..5235
                     /gene="ddpX"
                     /locus_tag="POB34_004289"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HDI5921138.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQVCPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHDRSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            5249..>6055
                     /locus_tag="POB34_004290"
     CDS             5249..>6055
                     /locus_tag="POB34_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI5921139.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTSVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQLFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYS
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNV"
BASE COUNT         1629 a         1459 c         1461 g         1506 t
ORIGIN      
        1 tatatcggta tcgccttcta taaagtgggt gaaccctcaa agatagagcc agactggatg
       61 attaacggcg gcgtaccgga actgaaaaag cagctcgatc ttaacgatgc ggtaccagaa
      121 attagcggca ccatcttgtt ccgtgaggac tatctgaata aaccgcagac tcaacaagcg
      181 gtcagctatc tgcaaagtcg ttggggcagt taaactccca ttcttacttt gtaacaagcc
      241 ggtgctttac ccaccggctt gttaccctct tgaactatcc cactactaat catgcttact
      301 aaagtcaaat taaccacgca attaaccaca cttagttaaa cagccataaa tcccctaata
      361 aaaatgtatg aatatgaaaa aacatacgat attcgctgtc accgaaaaat aattacgatg
      421 ggctatactc ttctgaagaa cagtagcgta gattaaaact gtcctaagtc gcttatttct
      481 tttcagtata tcttcatatt tcaggagaat atggtgcagt tagtcgatct cgcacgttgc
      541 gtttcgtttt gattccgtct gatttcccag ccttataagg gtgtgaatta tggagatgta
      601 ttttaaaaga atgaaagatg agtggaccgg acttgtcgaa caggcagatc ctctcattcg
      661 tgctaaagcc gcagaaattg ccgttgcgca tgctcattat ctgagtatcg agttttatcg
      721 aattgtccgc atcgacccgc atgccgaaga attcttgagt aatgaacaag ttgagcggca
      781 gttgaaaagt gcgatggaac gctggattat taacgtgctt tctgcccagg ttgacgatgt
      841 cgaaaggcta atacaaatcc agcataccgt cgcggaagtg catgcccgca taggaattcc
      901 ggtagaaatt gtcgagatgg ggtttcgggt gctgaaaaaa atcctctatc cggtaatctt
      961 ctcttcggat tattccgccg cagaaaaact tcaggtctat catttctcga ttaacagtat
     1021 tgatatcgcg atggaagtga tgacccgcgc gtttaccttt agtgacagta gtgcctcaaa
     1081 ggaagatgaa aactatcgta tcttctcgtt gctggaaaac gccgaagaag aaaaagaacg
     1141 gcaaatagcc tcaatacttt catgggaaat agatattatc tataaaatcc tgctggattc
     1201 tgatttaggc agtagtttgc ctttaagcca ggctgatttt ggcctgtggt ttaaccataa
     1261 aggtcgacat tattttagtg gcattgccga agtaggccat atctcccgtc tgattcagga
     1321 tttcgacggt atattcaatc aaaccatgcg taacaccagg aatctgaata acagaagtct
     1381 gcgggtgaaa tttttattac agataagaaa tactgtatcg caaattatta ccttgctgcg
     1441 tgaattgttt gaagaagtat cgcgccacga agtcggtatg gatgtactga cgaaattact
     1501 taaccgccgt ttcctgccga ctatcttcaa acgcgaaatt gcccatgcca accggaccgg
     1561 tacaccgctg tcagtgctga ttattgacgt tgataaattc aaagagatca acgatacgtg
     1621 gggccataac actggcgatg aaattctgcg taaagtctct caggcctttt atgacaacgt
     1681 ccgcagtagt gattatgttt tccgctacgg gggcgatgaa tttatcattg ttttgactga
     1741 agcttcggaa aacgaaacgt tacgtaccgc agaacgtatt cgcagtcggg tggagaaaac
     1801 caaactgaaa gccgcaaacg gcgaggatat tgccctctca ctttccatcg gtgccgccat
     1861 gtttaatggt catcctgact atgagcgcct cattcaaata gccgatgaag ctctgtatat
     1921 cgccaaaaga cgaggtagaa accgtgttga actctggaaa gccagtcttt agatgcgcca
     1981 ggatgcagag gtaatcatga agctaaccga tgcggaaaat gccgccgatg gcattttttt
     2041 ccccgccctt gagcaaaata tgatgggtgc ggtgttaatt aacgaaaatg atgaagtgat
     2101 gtttttcaac cccgccgcag agaagctctg ggggtacaaa cgtgaagaag tcattggcaa
     2161 taacattgat atgctgattc cgcgggattt gcgtcctgcg catcctgaat acattcgtca
     2221 taaccgtgaa ggcggtaaag cgcgcgtcga ggggatgagt cgggagctgc agctggagaa
     2281 aaaagacggc agtaaaatct ggacccgttt tgcgctatcg aaagtgagcg ccgaggggaa
     2341 agtttattac ctggcgctgg tacgggatgc cagcgtagaa atggcgcaaa aagaacagac
     2401 ccgacaattg attattgccg ttgaccatct cgaccgaccg gtgattgtcc tcgatccgga
     2461 acgccatatt gtgcagtgca atcgcgcatt taccgaaatg tttggttact gcattagcga
     2521 agccagcggt atgcagcccg atacactcct gaacattcct gaattccctg ccgataaccg
     2581 cattcgttta caacagttgc tatggaaaac cgcccgcgat caggacgaat ttctgctgtt
     2641 gacgcgcacc ggtgaaaaaa tctggattaa agcctctatc agcccggttt atgacgtgct
     2701 cgcgcatctg cagaacctgg taatgacttt ctcggatatc accgaagaac ggcagattcg
     2761 ccagcttgaa ggcaatattc tcgccgccat gtgcagcagc ccgccatttc atgaaatggg
     2821 ggaaatcatt tgtcgtaaca tcgaatctgt actcaacgaa tcgcatgttt cgctgttcgc
     2881 actgcgcaac gggatgccga tacactgggc gtcatcttcg cacggtgcag aagttcaaaa
     2941 tgcgcaaagc tggtcagcga ccattcgtca gcgtgatggc gcgcctgcgg ggatcctgca
     3001 aattaaaacc tcgtcaggag cagaaaccag cgcctttatc gaacgcgtgg cagatatcag
     3061 ccagcatatg gccgcgctgg cgctggaaca ggaaaaaagc cgtcagcata ttgaacaact
     3121 catccaattt gatccgatga ccggtctgcc aaatcgcaat aacctgcaca attacctcga
     3181 tgacctggtc gacaaagccg tctctccggt ggtgtatctc atcggtgttg accatattca
     3241 ggatgtgatt gatagccttg gctatgcgtg ggccgatcag gcattgctgg aagtggtcaa
     3301 tcgctttcgt gaaaaactca aaccggatca gtatctctgt cgtatcgaag gtacgcagtt
     3361 tgtcctcgtg agcctggaaa acgacgtcag taacattacc cagatcgccg atgagctacg
     3421 gaatgtggtc agcaagccga taatgattga cgataaaccc ttcccgctta ccttgagtat
     3481 tggcatcagc tacgacgtgg ggaaaaaccg cgattacctg ctctccactg cccataacgc
     3541 gatggattat attcgcaaga atggcggtaa cggctggcag ttcttcagcc cggcgatgaa
     3601 cgaaatggta aaagagcgtt tggttttagg cgcagcgctg aaagaaacga ttagcaataa
     3661 ccaactgaaa ctggtttacc agccgcaaat cttcgcagaa acgggtgaac tgtacggcat
     3721 cgaagccctt gctcgctggc acgatcccca gcatggtcat gtaccccctt cacggtttat
     3781 tcctctcgca gaagagattg gtgaaatcga aaatattggg cgctgggtca tcgcggaagc
     3841 ttgccgtcag ttagcagaat ggcgtagcca gaatattcat atcccggcgt tatccgtgaa
     3901 cttgtcggcg ctgcactttc gcagtaatca actgcctaat caggtgtctg atgcaatgca
     3961 agcctggggt attgacggcc accagctgac ggtggaaatc acggaaagca tgatgatgga
     4021 acacgatacc gaaatcttta agcgcattca gatcctgcgt gatatgggcg tgggtttatc
     4081 ggtagatgat tttggcacgg gcttttccgg attatcccgc ttagtcagtc ttccggtaac
     4141 ggaaatcaaa attgacaaaa gttttgtcga tcgttgtctg acggaaaaac gcatccttgc
     4201 cttacttgaa gccattacca gcattgggca aagcctcaat ttaaccgtcg tggcggaagg
     4261 cgtcgaaacc aaagagcaat ttgagatgct acgcaagatc cactgtcgcg ttattcaggg
     4321 atatttcttt tcccgccccc tgcccgccga agaaattcca ggctggatga gcagcgtgtt
     4381 accgctgaaa atctgacaaa ttcctctagc ccgcactcgc gggtattcat ttaacgtgac
     4441 actgtcactt aaatactgtg atttcagcta cgattccggg agattccttc cttaacttcc
     4501 cctcttgcac caaaactgca ctacgctgca caaatgttga tcaaaagtta aaaaatataa
     4561 ctttaagcca gcattctggc gcttctcccg gcatggcatg agatctgcat aagcggaaag
     4621 cgcagcaatt tttgtcttat acaggaaccg tttatgtcgg ataccaccga actggttgat
     4681 ttagccgtaa tcttccctga tctggagatc gaattgaaat acgcctgcgc tgataacatc
     4741 acaggtaaag ctatttatca gcaagcgcgt tgtctgttac acaaggatgc gattaccgcg
     4801 ctggcgaaaa gtatcagtat cgcccagctg tcaggcttac aactggtgat ttacgatgcg
     4861 tatcgcccac aacaagcaca ggcgatgttg tggcaagtct gcccagaccc gcaatatgtt
     4921 gttgatgtga cggtcggttc taatcacagc cgtggcacgg cgatcgacct gacgcttcgt
     4981 gatgagcacg ggaacatcct cgatatgggc gcagggttcg atgaaatgca cgatcgctcc
     5041 catgcttacc atccttccgt cccgcccgcc gctcagcgca atcgcctgtt gctgaatgcg
     5101 attatgactg gtggcggttt tgtcggtatc tccagcgaat ggtggcactt cgaattaccc
     5161 caggcagcga gttacccact gctcgctgat caattcacct gttttatatc ccctggcaca
     5221 cagcacgtca gttaatcagg aaactgtcat gaaaagatcg atatcgtttc gtcctacatt
     5281 gctcgcgctc gtccttgcca caactttccc ggttgcgcac gccgccgtac cgaaagatat
     5341 gctggtgatc ggtaaggctg ccgatccaca aaccctcgac ccggcggtga caattgataa
     5401 taacgactgg acggtgacct acccgtctta tcagcggctg gttcagtaca aaacggacgg
     5461 tgataaaggc tcaaccgacg ttgaaggcga tctggcaagt agctggaaag cgtctgacga
     5521 tcaaaaagag tggacgttca ccctgaagga taatgctaaa tttgccgatg gcacatctgt
     5581 cactgccgaa gcagtaaaac tctcttttga gcggttacta aaaatcggcc aggggccagc
     5641 agaagcattt cccaaagatt taaagattga tgctcccgac gaacatacgg tgaagtttac
     5701 ccttagccaa ctattcgcac cgttcctcta cacgctggcg aatgacggtg catccattat
     5761 caatccggcg gtgttaaagg aacatgcagc ggatgatgct cgcggcttcc tcgcgcaaaa
     5821 taccgccggt tccggaccat ttatgctgaa aagctggcaa aaaggtcagc aattagttct
     5881 ggtgccaaat ccgcattact ccggcaataa accgaacttt aagcgagtat cggtaaaaat
     5941 tatcggtgaa agtgcctccc gtcgcctgca gctctcccgt ggcgacattg acattgccga
     6001 tgcgctgccg gtggatcaac tcaacgccct gaagcaggaa aataaagtca atgtg
//
LOCUS       DAMZET010000214         5981 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.214, whole genome shotgun sequence.
ACCESSION   DAMZET010000214 DAMZET010000000
VERSION     DAMZET010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.214"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..367)
                     /gene="yciA"
                     /locus_tag="POB34_004291"
     CDS             complement(<1..367)
                     /gene="yciA"
                     /locus_tag="POB34_004291"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HDI5921140.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDP"
     gene            complement(472..1011)
                     /locus_tag="POB34_004292"
     CDS             complement(472..1011)
                     /locus_tag="POB34_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HDI5921141.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(1041..1784)
                     /locus_tag="POB34_004293"
     CDS             complement(1041..1784)
                     /locus_tag="POB34_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HDI5921142.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(1812..1901)
                     /locus_tag="POB34_004294"
                     /pseudo
     CDS             complement(1812..1901)
                     /locus_tag="POB34_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2141..2779
                     /gene="ompW"
                     /locus_tag="POB34_004295"
     CDS             2141..2779
                     /gene="ompW"
                     /locus_tag="POB34_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HDI5921143.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(2839..3345)
                     /locus_tag="POB34_004296"
     CDS             complement(2839..3345)
                     /locus_tag="POB34_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HDI5921144.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPTIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(3391..3891)
                     /locus_tag="POB34_004297"
     CDS             complement(3391..3891)
                     /locus_tag="POB34_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HDI5921145.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(3977..4156)
                     /locus_tag="POB34_004298"
     CDS             complement(3977..4156)
                     /locus_tag="POB34_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDI5921146.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(4537..5343)
                     /gene="trpA"
                     /locus_tag="POB34_004299"
     CDS             complement(4537..5343)
                     /gene="trpA"
                     /locus_tag="POB34_004299"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDI5921147.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(5343..>5981)
                     /locus_tag="POB34_004300"
     CDS             complement(5343..>5981)
                     /locus_tag="POB34_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HDI5921148.1"
                     /translation="YMLGTAAGPHPYPTIVREFQRMIGEETKAQILEREGRLPDAVIA
                     CVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRVGIYFGMKAPMM
                     QTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALEAFKTLCLHEGI
                     IPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILKARGEI"
BASE COUNT         1586 a         1407 c         1415 g         1573 t
ORIGIN      
        1 caggatcaac cgcgacatac ttaaataatg cttctgtcgc tttatagcgt tgcccgattg
       61 gttcagacgc tacttttttc acccacactt caatattaat gctgaccgat gtcgtccctt
      121 tctggacaca gcgtgcatag cagcacacca catcgccgac cgcaaccggc cgtaagaaag
      181 tcattccttc aacccgcaca gtcactacgc gaccgtgggc aatttctttc gccagaatag
      241 cgccgccaat atccatttgt gacattaacc aaccaccaaa gatgtcacca ttggcattgg
      301 tatcggcggg catggctaaa gtacgtaaaa caagatcgcc ctgagggacg ttatgtgttg
      361 tagacatggt aaaaccgact taaaaggaaa atcacaggcg cgatgctact atgattttcc
      421 tgaggagaac agaggagata cagcgccagc cccgaaggac tggcagtctg gttaggattt
      481 atcttcctgc ggcatgtggc ggtagatata gataccgctt aacaatgtga agattaaggt
      541 aagggcggtc aggccaaaga ctttaaagtt gacccaaata ttttgcggca gccagaatgc
      601 gatgtagatg tttgccagac cgcaaaggat aaagaaaaca gcccaagcca gatttagctt
      661 cgaccatacc ggttgcggca gcgtgagttc tttacccagc atccgctgaa ttagcggctt
      721 tttcatcacc cattggctga ctaacagggc tcccgcaaac agagcataaa tgactgtaac
      781 cttccattta ataaactcat cattgtggaa gaacagcgtc aagccaccga agacgaccac
      841 cagaacaaaa gtgatcaggg ccatcttctc aaccttacga aagcgaaccc agctatagat
      901 aagcacaatc gccgtggcga cgatcagcgc cgcagtagcc gcatagatgt cataaatctt
      961 gtaaaacgcg aaaaagacaa ccagcggtaa aaaatcaaga aactgcttca ttttacgatt
     1021 ccgtaatcaa atgcttaagg ttattggcga atcaacatat acaggcggaa cagatagatg
     1081 agcaatatgg cagaaatcag gttgctcaag gtgttcgcca gtacggcacc aatttccggg
     1141 gttaatgcgg caaaagaaga ggcaaaaagc agcaacagtg tttttgccag caaccagctc
     1201 agtactgcgg gtgccaccag acgcatattc gcccaagtca gccgcatact gctacgcatc
     1261 gaggcaaaaa tgcccatttt gtcctgtacc agcatcaccg gtgccagggc cagtaaaatg
     1321 gccatgataa ttcccggaac gaccaccagc atgatgccaa tctgtactaa aagggtagtc
     1381 agaaaaatca gaataaataa cttgggcaat atcggcgcac tggcaccaat agctcgcagc
     1441 gcactgactc tctgacccgc agacaccagc tggataatta atattacgcc tccggcgaga
     1501 atggcgttac cgattaatcc tgaaaaagtg gacgccgctg aagcctgcag caaaatttgt
     1561 tgctgttccg gtgacatatt ctgaaccagg tcgaacaacc cactactgcc gctaacgggc
     1621 acgccgtcat tgagctgcgc aagctgtgca tcactgggtg agaaaacatg ccctaacacc
     1681 actgtgataa acgcacatag caacgatacc aacagaatgg tcataaattg attacggaag
     1741 aaatttccgg tgtcacggta tacggactgc gccgtgatag acatgcactc tccttgagtt
     1801 ttatgcaggt gttaattagc gggcaattgt accctggtta agcctcgggt ggcagcatca
     1861 ggtcttgtat ggaaaagcat atctttgtaa agcggaggta attgctggcc ttcaggcgtg
     1921 ggcaaaccgc ggcaagtggc gatcgagtta atatttgcgt agagaaaata tttaaaaact
     1981 gatttaaatc acattaacca ggattctcaa tgcaacttct aaattaatcc agatcaataa
     2041 atggtgaatt atcatatgta atgtgatcta tgtaggatca tttgttactc caatgtaggt
     2101 atattcgtca cgtttttata accataacga cggagcggat atgaaaaagt taacagtggc
     2161 ggctttggca gtaacaactc ttctttctgg cagtgccttt gcgcatgaag caggcgaatt
     2221 ttttatgcgt gcaggttctg caaccgtacg tccaacagaa ggtgctggtg gtacgttagg
     2281 aagtctgggt ggattcagcg tgaccaataa cactcaactg ggcttgacgt ttacttatat
     2341 ggcgaccgac aacattggtg tggaattact ggcagcgacg ccgttccgcc ataaaatcgg
     2401 cacccgggcg accggcgata ttgcaaccgt tcaccatctg ccgccaacac tgatggcgca
     2461 gtggtatttt ggtgatgcca gcagcaaatt ccgtccttac gttggggccg gtattaacta
     2521 caccaccttc tttgataatg gatttaacga tcatggcaaa gaggcggggc tttccgatct
     2581 cagtctgaaa gattcctggg gagctgccgg gcaggtgggg gttgattatc tgattaaccg
     2641 tgactggttg gttaacatgt cagtgtggta catggatatc gataccaccg ccaattataa
     2701 gctaggcggt gcacagcaac acgatagcgt acgcctcgat ccgtgggtgt ttatgttctc
     2761 agcaggatat cgtttttaat tccacaaaaa aacgaccccg taatatacgg ggtcaataag
     2821 gacatggcat aaagtgtatt atttcttcgc ttctacgccg tcagtttcag agcgaattaa
     2881 aaatttctca gttgtttgcg gaatatgctg aatcagccag tcggccattt gcttttcctc
     2941 atttaaaatc gcttcgatgg ttggaattga agctgtatca ccggcatttt ttgctgctgc
     3001 taatagtgag gtgtaacagg cgatttcaaa ttgctcgaag acatatccgc taatagagcc
     3061 tttgactatt tcatcagaag ggaatatacc accgattgac tgcccaagcg cagccatttt
     3121 actcatggaa tctttaatga ctgaacgtga aatgtcatta cgatcaagaa tagtttccag
     3181 ttgaacaatc tggtttttgg tttcactaag atgttgttca atacgagcgc gtagttcagg
     3241 ataattatct atacggctgg ccatggattc aagcatagat tcggcttgct tttccattgc
     3301 gtgggcgtca cgtaaccagt catgataatg ttcaatacga ttcatgaaaa tgtcctcttt
     3361 ataaaatgac taaaagttaa agttcatatt tcaggcttta ttttcggctt tcttatttac
     3421 gttattaagg gccagatcag tcagtttgat gtcggtggcc ttttcttctt ccagggtctc
     3481 tttcagaagc ttcgctgctt tacgataacc taattgttca gccagcgtcg ctaatgtccc
     3541 gtaactggca atctcataat gctcgacttt ctgtgctgcg gcaatcagtg cggcatcacg
     3601 cacttcgttt ttctcggtac tttcgatgac ctcattagct tcttcaataa gaccttccat
     3661 tgccacacat ttcatgcgct taattttcag attcgattct gattccacaa cttgatcaat
     3721 acgttcaatc tgtccatgtg tttcctcgag gtgcgcatga aaagcctgac ttaatttttc
     3781 gtttgatgtt gctcttgcga gttttgccag tgcccgggtt aattgttttt ctgcgctgta
     3841 ggtatctgaa agcaggtgaa taaatacatc ttcaatggtc ttcatattca tatttttctc
     3901 cagtgaaatc actgcgagcc atacggcccg cagatgcaat tatctttatc agctgatatt
     3961 aataattaac ggtgaatcag gatttgcctg atttattacc accgctttgt tgaccgcctt
     4021 ttttacccgc ttcagatgcg cgttgcggat catttttaaa attaccgccg ctatgctgac
     4081 cgcctttacg gcctgcgtcg gatgccttct cacggtcttc ggcgaaattt cctgaaccac
     4141 cacgatgttc ggccatgtta tttctcccgt tgcgttgcat tgtttcatta atatgagtgt
     4201 tgtgtgtcga cactcattaa aattagtcgc taatgagaat tagtcaaatt aagcgcaacg
     4261 agaagataga gggaaaatat attttgagga acattctgga tatattaaca attacctgag
     4321 gaataagtga cttagagaaa attattgatt ttactggtgt tatgttgcgg gattaatttg
     4381 tttgattata ttactgttgg gcggaaaaat gacgtaagtt gacgttcgac cggggtaagc
     4441 gaaacggtaa aaagataaat attaaatgaa tttaggattt ttccggcttc attaaagaaa
     4501 gttaaaatgc cgccagcgga actggcggct gtgggattaa ctgcgcgtcg ccgctttcat
     4561 cggttgtaca aaaactttca gtgccgccag cattttctct ggctcattaa tatgttgctc
     4621 gatgatttta acaatggccg aaccagaaat cgcgcccgca gctcctgcat caatcgctgc
     4681 ttttacctga tccggggcgg aaataccaaa cccctgcaat ggaggtgcag cgttgtactc
     4741 tttcagcttc gcaaccagat gattgagggg taacgcggcg cggttttctg cgccggtcac
     4801 gcccgctcgt gacagcaaat aggtgtaacc acgaccgtaa gaggctatct ggcgcagcag
     4861 gtcgtcatcg gcatttggcg ggcagatgaa gataggtgcg acattatggc gcaacgcggc
     4921 ctggcggaag ggcgcggact cttcaattgg cacatcggca accagcaccg aatcgacgcc
     4981 gactttctcg cactgggcat aaaactcatc aatgcctttg ttaaacacca gattggcata
     5041 catcaacagg ccaatgggaa tggtcgggtg tttctggcga atcagtgcca gcatttcaaa
     5101 acattgtgcc ggagtcacac ctgccgcaaa ggcgcgcaga gtggcgtttt gaatggtcgg
     5161 gccatccgcc agtgggtcgg agaaggggat acctaactcc agcgcgtcag caccggcttc
     5221 aattagcgta tcgataattt tcaatgactg ctcaatgccc ggatcaccga gcgtgacgaa
     5281 aggaacgaat gcgccttctt tgcgctcctt caactgggca aacagagatt cgtagcgttc
     5341 catcagattt cccctcgtgc tttcaaaata tcgtgaacgg tgaagatgtc tttatcgccg
     5401 cgaccggaaa ggttaaccac cagtagctgc tctttatccg ggttttcgcg catcattttc
     5461 aacgcatggg ccagggcgtg ggaggattcc agcgccggga tgatcccttc gtgcaggcac
     5521 agcgttttga aggcttcaag ggcttcatca tcggtaatag acacgtaatc agcgcgtcca
     5581 gtgctgttaa gatacgcgtg ttgtgggccg acagacggga aatccagtcc ggcggagatg
     5641 gagtaagatt cttcaatctg cccgtcttcg gtttgcatca tcggcgcttt cataccgaaa
     5701 tagatgccca cgcgaccatg ttttaacggt gcgccgtgct cgccagtttc gataccgtga
     5761 ccacctggct ccacaccaat caggccgacg ttggtttcat tgatgaaatc agcaaacatg
     5821 ccgatggcat tcgaaccgcc gccaacacag gcgataacgg catccggcag gcgaccttct
     5881 ctttccagaa tctgcgcttt ggtttcttcg ccaatcatcc gctgaaactc acgcacaatg
     5941 gtcggataag gatgcgggcc agctgcggtg cccagcatat a
//
LOCUS       DAMZET010000215         5973 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.215, whole genome shotgun sequence.
ACCESSION   DAMZET010000215 DAMZET010000000
VERSION     DAMZET010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.215"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            103..1350
                     /gene="ybdG"
                     /locus_tag="POB34_004301"
     CDS             103..1350
                     /gene="ybdG"
                     /locus_tag="POB34_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HDI5921149.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(1418..2794)
                     /gene="pheP"
                     /locus_tag="POB34_004302"
     CDS             complement(1418..2794)
                     /gene="pheP"
                     /locus_tag="POB34_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HDI5921150.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(2896..>5973)
                     /gene="cusA"
                     /locus_tag="POB34_004303"
     CDS             complement(2896..>5973)
                     /gene="cusA"
                     /locus_tag="POB34_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HDI5921151.1"
                     /translation="LSIWGTWTIINTPVDALPDLSDVQVIIKTSYPGQAPQIVENQVT
                     YPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTDPYWARSRVLEYLNQVQGKLPAG
                     VSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQDWFLKYELKTIPDVAEVASVGG
                     VVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAGGSSIELAEAEYMVRASGYLQTL
                     DDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIAELNGEGEVAGGVVILRSGKNAR
                     EVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRAIDNLSGKLLEEFIVVAVVCALF
                     LWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMSLGGIAIAVGAMVDAAIVMIENA
                     HKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPALFISLLIITLSFIPIFTLEGQEG
                     RLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGKIPPESSNPLNRFLIRVYHPLLL
                     KVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQINEGDLLYMPSTLPGISAAEAASM
                     LQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMVETTIQLKPQEQWRPGMTMDKII
                     EELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIGIKVSGTVLADIDAMAEQIEEVA
                     RTVPGVASALAERLEGGRYINVEINREKAARYGMTVADVQLFVTSAVGGAMVGETVEG
                     IARYPINLRYPQSWRDSPQALRQLPILTPMKQQITLADVADIKVSTGPSMLKTENARP
                     TSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSVAFSGQFELLERANHKLKLMVPM
                     TLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWLLWWMGFHLSVATGTGFIALAGV
                     AAEFGVVMLMYLRHAIEAVPSLNNPQTFSEQKLDEALYHGAVLRVRPKAMTVAVIIAG
                     LLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFIIPAAYKLMWLHRHRVRK"
BASE COUNT         1460 a         1716 c         1450 g         1347 t
ORIGIN      
        1 tcttgccggg catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt
       61 ttcagccgtc atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc
      121 caggttgaag atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc
      181 ggtattattt ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg
      241 accttcgaaa aacgtgccat cgccagttca cggctttggt tgcaaatcat tacccagaat
      301 aaactcttcc accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta
      361 ttctggctgc aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg
      421 atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg
      481 cagaaattcc cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg
      541 atcggcgcaa tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg
      601 attctgatca gcggtcttgg tgcaatggct gccgtgctga tgttggtttt taaagatccg
      661 attcttggtc tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac
      721 tggctggaga tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc
      781 gtcaaagtgc gtaactggga caataccatt accactattc ccacctggtc tctggtttct
      841 gactccttta aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt
      901 atcagtattg atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat
      961 aaagcgcact tgttaaaacc ttatttaacc agccgccatc aggaaattaa tgagtggaat
     1021 cgccagcaag gttctacgga gtcgatatta aacctgcgcc gaatgaccaa tattggaacc
     1081 tttcgtgcct atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc
     1141 ttaatggtac gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt
     1201 actaacaccg tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata
     1261 tttgcgattg tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt
     1321 cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga
     1381 gagcggacat cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa
     1441 atgccacaaa caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat
     1501 ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc aatgcaaaga tagttgccga
     1561 acggatagag cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gctgcacgaa
     1621 aacgcagatg cgccagacag atcataatcc agttcaacag cagcgttgct accaccagcg
     1681 ccatcagcag accaaacgct ttttgcggca gcagatagtt gattaatacc accagcgaag
     1741 tgatcgctcc ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca
     1801 aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa
     1861 ccccgctgtt atacactgac agcgatgcta ccagaatgac gaagttcagc gcagaagcta
     1921 ccacgttgct gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt
     1981 ccacccacgg atagagcgcc agtaaaacca ccagtgaacc gatgtaaaac agcaggatgc
     2041 gatacaccac ctgatttacc gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg
     2101 cagtaatccc aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa
     2161 tcagcccatt ccagccggtg gcaaagaaac caccgtagcg ccagaggttg tcgatactgg
     2221 ctttctcgcc gccgtgacca gaaaacagca gccacaggcc aaagccgatc ataccgatga
     2281 tcgccagcac tttaatcagc gcaaaccaga actcggtttc accatataag cgcacgttca
     2341 ccaggttaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat
     2401 ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca
     2461 cgaacattac ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat
     2521 aggcaaagtg ggcaaatgaa ccggataccg gctcctcaac caccatttcg ccaagctggc
     2581 gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg
     2641 ccatctgaat cgccgggcca atgccaagaa acagaccagt accaattgcg ccacccagcg
     2701 caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg
     2761 cagtatcttc cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt
     2821 tgaggggcac cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa
     2881 cggggtatcc tgcttttatt tccgtacccg atgtcggtgc agccacatca gcttatacgc
     2941 cgccgggata ataaacagcg acagcaaagg tgcggtgatc atgccgccaa tcatcggcgc
     3001 ggcaatccgg ctcatcacct ctgaaccagc ccccgttccc cacagaatcg gcagcagacc
     3061 ggcgataatc accgccaccg tcatcgcttt cgggcgcacg cgcaggaccg cgccgtgata
     3121 taacgcctca tccagcttct gctcgctgaa tgtttgtgga ttattcaacg acggcacggc
     3181 ctctatggcg tgacgtaaat acatcagcat caccacgcca aattcggcgg cgaccccggc
     3241 gagggcgata aagccagtgc ccgtcgccac ggaaagatga aagcccatcc accacagcaa
     3301 ccagatgccg cccaccagcg caaacggtac gctgctgata atcagcaacg cttcgcccac
     3361 ccgacggaac gccagataca acagcacgaa gataatcatc aacgtcatcg gcaccatgag
     3421 tttaagctta tggttggcgc gctccagtag ctcgaactgc ccggagaatg ccacgctggt
     3481 gcccggtttt aactgcactt tttcagctat cgctttttgc aaatcgtgaa ccaccgacac
     3541 catgtcacga tcgcgggcat cgatataaat ccagctcgtc gggcgcgcat tctcggtttt
     3601 cagcatcgac ggtccggtag agactttaat gtcggccacg tctgccaggg tgatttgctg
     3661 cttcatcggc gtcaggatcg gcagctggcg cagtgcctgc ggactatcgc gccagctttg
     3721 cggataacgc agattaattg gataacgggc aatcccttcc accgtttcgc caaccatcgc
     3781 cccgcccacc gcagaagtca caaacaactg cacatccgcc accgtcatac cgtaacgtgc
     3841 ggctttttca cggttaatct caacgttgat atagcgccca ccttccagcc gctcggcaag
     3901 cgcagaagct acgcctggca ccgttcgcgc tacttcttca atttgctcag ccatcgcgtc
     3961 gatatccgcc agcacagtgc cggaaacttt aatgccgatg gggcttttaa tgccggttga
     4021 gagcatatcg atacggttac gaattggcgg cacccacaga ttcgccagcc ccggcagccg
     4081 cacggtgtta tccagttcct caatgatttt gtccatcgtc atgcctggcc gccactgctc
     4141 ctgcggctta agctggatgg tcgtttctac catctccagc ggagcagaat cggtggcggt
     4201 ttccgctttc ccggttttgc caaatacccg cgccacttca ggtacgctca taattagctt
     4261 gtcggttttt tgcagcatac tcgccgcctc tgctgcggaa atccccggca gcgtcgatgg
     4321 catatacaac aagtcgcctt cattgatctg cggtaaaaat tccccgccaa ctttattgag
     4381 cggccagaga accgtcagca ccgaaagcgc cgccaccagc agcgtggttt tcggccagtg
     4441 cagtactttc agcaacagcg gatgataaac acgaatcaaa aagcgattga gcgggttact
     4501 gctttccggc ggaattttgc cacggatcca gtagcccatc aggatcggga tcactacgat
     4561 cgccagcagc gccgcacccg ccatcgcata cgttttggtg aacgccaacg ggccaaacag
     4621 acgcccttcc tgcccttcca gggtgaagat cgggataaac gacaacgtga taatcagcag
     4681 actgataaat agcgccggcc ccacttcaac agacgcatcg gtgatcacct gccagcgcgt
     4741 tttattatcc agcgtggcgt caggatgctg gtgctgccac tcttccagcc gtttatgcgc
     4801 attctcgatc atgacgatag cagcatcgac catcgccccg acggcaatcg caatgccacc
     4861 caacgacata atattggcat tcagtccctg gaagtgcatg acaataaaag caatacacaa
     4921 ccccagcggc aacgaaataa tcgccaccag cgccgagcgc acatgccaga gaaacagcgc
     4981 acagactacc gccaccacaa taaactcttc cagcaacttg ccgctgaggt tgtcgatggc
     5041 gcggtcaatg agctggctgc gatcgtatgt tgtaactatc tccacgcctt ccggcagact
     5101 acttttcagc gtttccagtt tgtccttcac ggcggcgatc acttctcggg cgtttttgcc
     5161 ggatcgcagg atcaccaccc cgcccgccac ttcgccttcg ccgtttagtt cggcaatgcc
     5221 ccggcgcatc tccgggccaa tctggacctt cgcaacatcg cgcagataaa cgggcacgcc
     5281 attttcactg gcttttaaaa cgatgtgatt aaagtcgtcg agcgtttgca gatagccgct
     5341 ggcgcgcacc atatattccg cttccgccag ttcgatcgac gaaccgcccg cttcctggtt
     5401 tgaagcatcc agcgcgcttt ttacttcggc gagactgatg ccatactgcg ccaggcgctg
     5461 gggatcgata accacctgat actctttcac cacaccgccc accgacgcca cttccgcaac
     5521 gtcagggatg gttttcagct catatttgag aaaccagtcc tgtaatgagc gtaaatcggc
     5581 cagatcgtgc ttaccgctgc gatccaccag tgcatattca tagatccagc caacacccgt
     5641 ggcatctggc cccagctcgg cgctgactcc cgcaggcagc ttaccctgta cctggttgag
     5701 atactccagc acccgcgaac gcgcccagta cggatcggtg ccatcttcga aaatgacata
     5761 cacataagag tcgccaaact gtgagaaacc gcgcacagtc ttcgcgccag gcaccgacaa
     5821 catggtggtg gttagcggat aagtcacctg attttcaacg atttgtggtg cctgaccggg
     5881 atagctggtt ttaataataa cctgcacatc ggagagatcc ggcagcgcat ccactggcgt
     5941 attaatgatg gtccaggtgc cccagatgct cag
//
LOCUS       DAMZET010000216         5948 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.216, whole genome shotgun sequence.
ACCESSION   DAMZET010000216 DAMZET010000000
VERSION     DAMZET010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.216"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..314
                     /locus_tag="POB34_004304"
     CDS             <1..314
                     /locus_tag="POB34_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HDI5921152.1"
                     /translation="PVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVL
                     RFPEVQMHEILSDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGS
                     K"
     gene            626..834
                     /locus_tag="POB34_004305"
                     /pseudo
     CDS             626..834
                     /locus_tag="POB34_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1266..1829
                     /gene="fimA"
                     /locus_tag="POB34_004306"
     CDS             1266..1829
                     /gene="fimA"
                     /locus_tag="POB34_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDI5921153.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            2190..2900
                     /locus_tag="POB34_004307"
     CDS             2190..2900
                     /locus_tag="POB34_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDI5921154.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            2942..5593
                     /locus_tag="POB34_004308"
     CDS             2942..5593
                     /locus_tag="POB34_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDI5921155.1"
                     /translation="MTAIRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSATNGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRNGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDK
                     NSNCIVDYKLPEVSPGTLLNQQTAICR"
     gene            5607..>5948
                     /locus_tag="POB34_004309"
     CDS             5607..>5948
                     /locus_tag="POB34_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDI5921156.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLG"
BASE COUNT         1703 a         1254 c         1320 g         1671 t
ORIGIN      
        1 ttccggtcct tggcggtacg ggaatacaca tcagcgatct caaactggca atggggctta
       61 acgcatccaa aggcaaaaaa acggcaatcg ataaaattta tccgcgacat tttttggcga
      121 cagcaaaggt gctgagattc ccggaagtgc agatgcatga aatcctaagt gactttgcca
      181 gaatgattcc ggcagcactg gataacgtga agacttcatt accgacagat tttccggaga
      241 acgtggtgac ggcagttgaa accaatgtgt tgaggttgca tggacggtta agccgagaat
      301 acggtagtaa gtgagatatg gggtttggtc gttgttaatg agcacgacaa tcatgaccgc
      361 caacgactgg attcttcctt ggtttatact ggtgcgcata atgtatatta tgttaaatca
      421 tgtctgagtg atgcgaagtg cttttctggt agtcgttatt cgttcagggt tcaagaactt
      481 aagcctctcc tctttcataa ttggcctgtg catggagaaa tagcctggtt attccctgcc
      541 ccatgctaag agcgcagtgt ctgtcgcata gacactgcat acgaaaacac cagagggtgc
      601 tgttacttat ttaaggacgg atcaagcggg aatgagtcca ctccggtatc agaaatggct
      661 acgtctcaat gaagtcaggc gaccgatgct gaatgaacat tacgatgtca ccacagcagc
      721 ctatgctgtc ggttacgaaa gctatcccat ttcagtcggg aatattcgcg gatgtttgga
      781 gagtcaccca agagagatat taccaggtta agaaagtctg tcggtcagct ttaataaacc
      841 ctgcacttat ctgtttagag caattcggtg ttagtttcag caagcaaaca ttaaccatag
      901 ctaatgattt atagccatat taaccattgg tagcccttga cctttcctga cacttccctt
      961 gttcgcaata atattctgtt tagtcacggt ttataaataa catgcataat gaaatcgtca
     1021 tgttatttag cgacataaaa tgtttttgca aatactctct tcaacaagtg cacaataaac
     1081 ttgttgtgat taggtgtcct tgtgatttaa agatattatc gttcttcgtg aaatgtaata
     1141 accctggaaa ctatattctc agggcaacaa ccaatcaaga atgattcttg tttttaagca
     1201 ataacagaat gacttaattg ttaactctct ttattcagct acttaaatat aaattttgga
     1261 gaattatgaa actcaaacat gttggtatga ttgtcgtttc tgtgttggcg atgtcgtctg
     1321 ctgcggtaag cgcagccgag ggtgatgaat cagtaacgac cactgttaat ggcggtgtta
     1381 ttcattttaa aggtgaagtg gtaaatgccg cttgtgcaat tgattcagaa tcaatgaacc
     1441 aaacggttga gctgggtcag gttcgttctt ctcgcctggc taaagcgggt gacctcagct
     1501 ccgccgttgg cttcaatatc aagctgaatg attgtgatac caatgtttcc agtaatgcag
     1561 ctgttgcatt cctgggtact actgtcacca gtaatgacga tacgttagcg ctgcagagtt
     1621 cagcggcagg ctctgcccaa aatgtcggta ttcaaatttt ggaccgtacg ggtgaggtat
     1681 taatacttga tggggccact tttagtgcta aaaccgactt gattgatggc acgaatatac
     1741 tgccattcca ggctcgttat attgctctcg ggcagtccgt agctggtact gcaaacgctg
     1801 atgcgacctt caaagttcaa tatctataat tcaatcttaa ggtggctata cgtcatctga
     1861 aattgaaaca gggtgtgtgt ggcatggcta cgcacacccg agcaatttac caatattcat
     1921 ctcattttta atgagaaatg gtttgtattt ggagcgctat gaaaaatcac ataatatcta
     1981 aactatcaat aaacaacagt agttaaactt actgttattt aaaatattta ctttgtagca
     2041 aggttttcct attaaaagag aaccaacatt tattacaaac gcattaagtc tggaaacccg
     2101 gcgactcgcc aatgtattag caacattaat cgattgtctg atgtaacggt tttcccgctt
     2161 atcctcttaa cacatggaga atgcatcaga tgcaaactac gcgtaccccc tactctattt
     2221 cctttatggc gacagtgtta cttttattgc tctttgcttg ccatagcacc gttgccaacg
     2281 cagctgtcgc actgggtgcg acgcgggtaa tttatccggc aaaccagaaa caggttttat
     2341 tgcctgtcac gaataatgat cctgccagtg tctatctcat tcagtcatgg atagaaaatg
     2401 caggtgacca aaaagacacc cagtttgtca ttaccccacc gcttttttcc atgcaaggta
     2461 aaaaagagaa tacgctgcgg ataatcaacg ctactaatca tcagttacca ggcgaccgtg
     2521 aaagcctgtt ctgggtgaat gttaaagcta ttcctgctat ggaaaaagac cagaagaatg
     2581 agaatacatt acagctggca attattagcc gcataaaaat gttttatcgc cccacgaacc
     2641 tggcaatggc acctgaagaa gcgcctgcaa tgcttcggtt tcgtcgcagc ggtagcaaac
     2701 tcacgcttat taatccaacg ccctatttca ttaccgtaac aaatatgaaa gcaggaaata
     2761 gcaatctccc aaacaccatg gtgccgccaa aaggtgaagt atcggtggat atcacgcatg
     2821 ctgcaaccgg ggatatcagt tttcagacga tcaatgatta cggtgcatta actccgcgta
     2881 ttaaggcaac catgcagtaa gccagtgacc taattaataa taaactcaat cggaattaat
     2941 aatgacagcc attcgtgccg catttaaggc atatcgtatg catcaggtgt taataatgcc
     3001 tcgctttgca cggcttacca ttgcccttgg cttagcaact gcggtatttc ccgttgacgc
     3061 agagtattat tttaatccac ggtttttaag caatgatctt gctgaatctg tcgatttatc
     3121 cgcctttact aagggacgcg aggcaccacc tggtacatat cgggtcgaca tttatttgaa
     3181 cgatgaattt atgactagtc gggatattac ttttattgca gatgataata atgcagaact
     3241 catcccatgc ctgagtacag atcttcttgt cagtcttggt ataaaaaaat cagcgttatt
     3301 agacaataaa gaacattcgg cagaaaaaca tgtgccggac aacagcgcat gcacaccact
     3361 tcaggatcgt ttggccgacg catcaactga gtttgatgtt ggtcagcaac atttgtcact
     3421 ctccgttccg caaatttatg ttggcagaat ggctcgcggc tatgtttctc ctgatctgtg
     3481 ggaagaagga ataaatgctg ggcttctaaa ctatagtttt aatggtaatt ctattaataa
     3541 tcgtagtaac cataatgcag gaaaatcaaa ctatgcatat ttgaatttac agagtggcat
     3601 caacattggt agttggcgac tacgcgataa ctcaacgtgg agttataaca gtgggagtag
     3661 caattcatct gacagcaata aatggcagca tatcaatacg tcggctgaac gtgacattat
     3721 tcccttacgc tcacggttaa cggtaggtga tagttatacc gatggcgata tttttgatag
     3781 tgtgaacttt cgtggcctca aaataaattc aacagaagcg atgttgcccg atagccaaca
     3841 tggtttcgct cctgtgattc atggtattgc ccgtggaacc gcacaagtga gtgtaaaaca
     3901 gaacggatac gatgtttatc agactactgt tccacccggc ccttttacta ttgatgatat
     3961 caactctgcg accaatggtg gtgatctgca agtaaccata aaagaggcag acggcagtat
     4021 tcagacatta tatgttcctt attcgtctgt tccggttctc caacgtgctg gatatacgcg
     4081 ttatgcgctt gccatggggg aatatcgtag tggaaataac ctgcaaagct ctcccaagtt
     4141 catacaaggt agcttgatgc atggtctgga aggaaactgg acaccttatg gcggaatgca
     4201 aattgcagaa gattatcagg ccttcaacct tggtattggg aaagatttag gactttttgg
     4261 tgccttttct ttcgatatca cgcaggccaa tacgacactt gcagatggca cccgtcacag
     4321 tgggcaatcg gttaaatccg tctacagcaa atccttctac cagacaggaa ccaatatcca
     4381 ggtcgcagga tatcgctatt ctacgcaagg tttttataac ttatccgaca gtgcctacag
     4441 tcgaatgagt ggttacaccg tcaagcctcc taccggagac accaatgagc agacacaatt
     4501 tattgattat tttaatctgt tctacagtaa gcgtggtcag gaacaaataa gcatctctca
     4561 gcagcttgga aattacggta cgacattttt cagtgccagt cgccaaagtt actggaacac
     4621 gtcacgcagc gaccagcaaa tatcatttgg attaaatgtg ccgtttggtg atattacgac
     4681 ttcgctgaat tacagctatt ccaataatat atggcaaaac gatcgggatc atttactcgc
     4741 ttttacgctt aatgttccct tcagtcattg gatgcgtaca gacagtcagt cggcatttcg
     4801 taattcaaac gccagttaca gtatgtcaaa cgatttgaaa ggcggcatga ccaatctatc
     4861 gggggtttat ggcactctgc tgccggataa taacctgaat tatagcgttc aggtcggtaa
     4921 cacccacgga ggtaatacat cgtctggcac cagtggttac agttctctta attatcgtgg
     4981 agcttacggt aatacgaatg tcggttacag tcggaatggt gacagcagcc agatttatta
     5041 cggaatgagt ggtgggatta ttgctcatgc tgatggcatc acctttggac agccgctggg
     5101 cgacacaatg gttctggtta aggctcctgg cgctgataat gtcaaaatag agaaccagac
     5161 cggaattcat accgactggc gtggctatgc aatattacca tttgcgacag aatatagaga
     5221 aaatcgtgtc gctcttaacg cgaattccct tgcagataat gttgaactgg atgaaaccgt
     5281 agtcactgtc atcccaactc acggtgctat tgccagagca acatttaatg cacaaatcgg
     5341 cgggaaagta ttaatgacgt tgaagtacgg taataaaagc gttccattcg gtgcaattgt
     5401 cactcacgga gagaataaaa atggcagcat tgtcgcggaa aatggtcagg tttatctgac
     5461 tggacttcca cagtcaggga aattacaggt ttcatggggc aatgataaaa actcaaactg
     5521 tattgtcgat tacaagcttc ctgaagtctc tcctggaacc ttgctgaacc agcagacagc
     5581 aatctgtcgc taaggaacaa tagattatga aatacaataa cgttattttc ctcggtttat
     5641 gtctggggtt aaccacctat tctgctttat ccgcagatag cgttattaaa attagcgggc
     5701 gcgtcctcga ttatggctgc acagtctcat cggattcgct taattttacc gtagatctcc
     5761 aaaaaaacag tgccagacaa tttccaacga ccggtagcac aagtccagcc gtcccttttc
     5821 agattacgtt aagtgaatgc agcaaaggga caacgggggt tcgggttgca tttaacggta
     5881 ttgaggatgc agaaaataat actctgttga aactggatga gggaagcaat acggcctccg
     5941 gtttaggt
//
LOCUS       DAMZET010000217         5947 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.217, whole genome shotgun sequence.
ACCESSION   DAMZET010000217 DAMZET010000000
VERSION     DAMZET010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.217"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(55..1056)
                     /gene="gap"
                     /locus_tag="POB34_004310"
     CDS             complement(55..1056)
                     /gene="gap"
                     /locus_tag="POB34_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDI5921157.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            1245..1775
                     /gene="cybB"
                     /locus_tag="POB34_004311"
     CDS             1245..1775
                     /gene="cybB"
                     /locus_tag="POB34_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HDI5921158.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            2020..2193
                     /locus_tag="POB34_004312"
     CDS             2020..2193
                     /locus_tag="POB34_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921159.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(2265..2414)
                     /gene="hokB"
                     /locus_tag="POB34_004313"
     CDS             complement(2265..2414)
                     /gene="hokB"
                     /locus_tag="POB34_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HDI5921160.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            2813..4453
                     /gene="trg"
                     /locus_tag="POB34_004314"
     CDS             2813..4453
                     /gene="trg"
                     /locus_tag="POB34_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HDI5921161.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(4491..5414)
                     /locus_tag="POB34_004315"
     CDS             complement(4491..5414)
                     /locus_tag="POB34_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI5921162.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     VEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            5631..>5947
                     /locus_tag="POB34_004316"
     CDS             5631..>5947
                     /locus_tag="POB34_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI5921163.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPV"
BASE COUNT         1548 a         1366 c         1505 g         1528 t
ORIGIN      
        1 tttacagtcg taatgagtaa aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag
       61 tttagcgaat ttttcgaggg tgcgaataag ctgcgtgacg aagccatatt cgttatcgta
      121 ccaggcgacc gttttcacca gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc
      181 atcaaacacc gaaccgaaat ggctgccaat gatatcggaa gagactattt cttcatcggt
      241 ataaccaaat gactcgttat tggtggttgc ttgtttaagt gcgttattca cctcttcggc
      301 agtcactttt tttccgagaa tcgataccag ttcagtgacc gaacctgttt tcaccggcac
      361 gcgttgcgca tgacctttca gtttgccgct cagttccggg atcaccagac caatggcttt
      421 tgccgccccc gtagtgtggg gaatgatatt ttctgccgct gcgcgtgaag cacgtaaatc
      481 tttaccacgt gggccatcca ccagtgactg ggtgccagta taggcatgaa tggtcgtcat
      541 cgtgccgact tctatcccga aactgtcatg caaggctttg gccatcggcg caagacagtt
      601 agtggtgcat gacgccacgg aaacaatggt gtcgttgcca tccagagtgt cgtcattgac
      661 gttataaacg atagttttca tttcaccggc aggggcggaa atcaacacct tcttcgcacc
      721 agcatcaaga tgcgcctgcg atttctcggc ggaggtataa aagccagtac attcgacaat
      781 gatttctgca cctttcgctt tccacggaat atttttagcc tctttttcgg cgtaaaccgc
      841 gatacttttc ccatcaacga taagtgaatc ttccgtaaaa tcaacgctcc aggggaatgg
      901 tccgtagttt gaatcatgtt tcagcaggta ggcgagaatt tttggggaag tgagatcatt
      961 aatagcgaca acgtctatgt tgcttttgac ttcaagtaat cgacgcaaca ccagtcgacc
     1021 gatacgacca aaaccgttaa taccaacttt actcatggtt ttctcctgtc aggaacattc
     1081 ggatgaaaat tgatcctttc caggcttaga ccaggattgt gggatgggca atctccattc
     1141 ttacagtgaa acgtaacgta ctgaaaacgg gtgaacaata tttaatgaaa ttttgagaaa
     1201 agcccgttat gttaacggaa aattatgtta aagcaggaaa tgttatggaa aataaatact
     1261 caaggttaca aatcagcatt cactggctgg tctttttgct ggttatcgca gcgtattgcg
     1321 caatggagtt tcgtggtttc ttcccacgta gcgatcggcc actcatcaac atggttcatg
     1381 tttcctgtgg tatctcaatt ctcgtgctga tggtggttcg tcttttgtta agactgaaat
     1441 acccaacccc gccgattata cctaagccaa aaccgatgat gacgggactg gcgcatttgg
     1501 gacatttggt gatttatctc ctgtttattg cgctgccagt gattggtttg gtgatgatgt
     1561 ataaccgggg caacccgtgg tttgcgtttg gtttgacgat gccttacgct tcagaggcca
     1621 atttcgaacg ggttgatagc ttaaagtcgt ggcatgagac gctggcgaat ctgggatatt
     1681 ttgtcatcgg gttgcacgct gcggctgcac tggcacacca ctatttctgg aaggacaaca
     1741 cacttctacg catgatgccg cgtaaacgtt cctgaaggat atttaaagaa aacgcctgta
     1801 ctaaaaccga cccgtggtac aggcgaagaa tacgggtcta catcggaagc gcctattata
     1861 tttatttgta tgacaaataa aaccataatc cttgcccata cgtccatctg gcttattttt
     1921 aatcaattca cccgatcttt gatctcatca acggtatcaa aataaaccag cgtataacgt
     1981 tcaaatcgaa cataagattc gataaaccat ggaggttata tgaaaaaact ggcacttatt
     2041 ttgtttatgg gaacgcttgt ttccttttat gccgatgccg ggcgcaaacc ctgttctggt
     2101 tcgaaagggg ggatctcaca ctgtacggca ggcggcaaat ttgtctgtaa tgatggttct
     2161 attagtgcct cgaaaaaaac atgcactaac tgaagtgtaa aaggggtgcc atgagaaaat
     2221 gaatctgcta gtcagatgcg cggggaaaat ccccgcgctt gcccttacct ggacgtgcag
     2281 gccatgagcg cagcaacctc cttatcaccg tcccggaacc gcagttcgta gagcgtttgt
     2341 cgggtcagga gtgtgaatgt cagaatcgta atgcagataa tgagcagaca caccaccaga
     2401 gggttgtgct tcatagcctt tctccttgcc ggatggcggg taagaggcta agatctgaat
     2461 tgctaggttc attcgttggc ctcggttgat agaaatatcg gtcggggcct tcgtctttct
     2521 gattcccggt tagcctgaaa acagaaagtc tcaggcaccc gcaggcatcc tatgaggttt
     2581 ccttagggac gaaaataatc acttcacgaa attgcgtgct gttttccaga atttttcgtc
     2641 attcggatta gccagtttag ccattcgtta ctctcttcat tccaatagca ttaattttct
     2701 ctgcaataat tgttgtaaaa atgtgacgca aagaggtttt tggtcataag taattaccgt
     2761 caagtgccga tgactttcta tcaggagtaa acctggacga gagacaacgg taatgaatac
     2821 aactccctca cagcgattag gttttttgca tcacatcagg ctggttccgt tatttgcctg
     2881 cattcttggc ggtatcttag ttctattcgc attaagttct gctctggctg gctatttcct
     2941 ctggcaggcc gatcgcgatc agcgtgatgt tactgcggag attgagattc ggaccgggtt
     3001 agcgaacagt tcagattttt tgcgttcagc ccggatcaat atgattcagg ccggggctgc
     3061 gagtcgtatt gcggaaatgg aagcaatgaa gcgaaatatt gcgcaagccg aatcggagat
     3121 taaacagtcg cagcaaggtt atcgtgctta tcagaatcga ccggtgaaaa cacctgctga
     3181 tgaagccctc gacactgaat taaatcaacg ctttcaggct tatatcacgg gtatgcaacc
     3241 tatgttgaaa tatgccaaaa atggcatgtt tgaagcgatt atcaatcatg aaagtgagca
     3301 gatccgaccg ctggataatg cttataccga tattttgaac aaagccgtta agatacgtag
     3361 caccagagcc aaccaactgg cggaactggc ccatcagcgc acccgcctgg gtgggatgtt
     3421 catgattggc gcgtttgtgc ttgccctggt catgacgctg ataacattta tggtgctacg
     3481 tcggatcgtc attcgtccac tgcaacatgc cgcacaacgg attgaaaaaa tcgccagtgg
     3541 cgatctgacg atgaatgatg aaccggcggg tcgtaatgaa atcggtcgct taagtcgtca
     3601 tttacagcaa atgcagcatt cactggggat gacagtaggg actgttcgac agggcgcgga
     3661 agagatttat cgtggcacca gcgaaatttc agctggcaat gcggacctgt catctcgcac
     3721 cgaagaacaa gcggcggcta tcgaacaaac tgccgccagc atggagcaac tcactgcgac
     3781 ggtgaaacag aatgcggata acgcgcatca tgccagcaaa ctggcgcaag aggcttctat
     3841 taaagccagc gatggcgggc agacggtttc cggtgtagta aaaacgatgg gcgctatctc
     3901 cacgagttcg aagaaaattt ctgagatcac cgccgtcatc aacagtattg ctttccagac
     3961 gaatattctg gcactgaatg ctgccgttga agccgcgcga gcgggtgagc aagggcgtgg
     4021 atttgccgtt gtcgccagcg aagtacggac actcgcaagt cgcagcgctc aggcggcgaa
     4081 agagattgaa ggcttgatca gtgaatcagt caggttaatt gacctggggt cggatgaggt
     4141 ggcaacggcc gggaaaacca tgagcactat tgttgatgcc gtcgcgagtg tcacacatat
     4201 catgcaggaa atcgccgccg cctcggatga acaaagtaga ggcataacgc aggttagcca
     4261 ggcgatttct gaaatggata aggtgacgca acagaatgct tctctggtag aagaggcctc
     4321 agcggcggcg gtgtcccttg aagaacaggc ggcacgatta actgaggcgg tggatgtatt
     4381 ccgtctgcac aaacattctg tgtcggcaga acctcgcgga gcgggtgaac cagttagttt
     4441 cgctacggtg tgaaaatgtt caaggaggga tcgacagatc ccttcacctt tcagaacggc
     4501 attgattttc gaatagcgtt aatcatcaac tggcaaccag aagagaacgt cgcatctacg
     4561 cgggtcagta ttccaatcgg ttcgactgca ccatgtcccg gaacaggcag ggccaccagc
     4621 gtggcatgac gcaggtcgtc ttttacagcg ccagaaggga caaaccacac gtaatcgtat
     4681 tcaaccgtaa gttgacgaga tagcgaagca gacagcgttt cgatacaacc cgaaggaatt
     4741 ttacagccct ggctctgcac taatgcatct gaatgctggc gtggcgcagt gccttctggt
     4801 gatacaacga ccggccattc cagcacccgg cttagcgtta cgttctcctg aagtagcggg
     4861 tgattagggc ggacaaccag cttcaacgat tcaagaaaca gcagttcgta attaagcccg
     4921 gtcatcagtt caggatctga catccgacca atgccgatat cgatttcccc ggtttttaaa
     4981 cccgccagaa tcatagggtt actcattgtc gcaacttgca aggtcgtctc tttttgttgc
     5041 tgatgaaact gacctataac cgaaggtaat atccccagtg ccgcagtagg tagtgcacca
     5101 accctgacga catcattatt aagaccttct ttacgatgaa gcgactgtcc ggcagtgttg
     5161 atggcgtcaa gaactctgac tgcatgcgtt aaaaattgtt cgccgggtaa ggtaagttgc
     5221 gccccctgac gaccacgctc aaacaagcga gcgccagtca gctgctccag ttcattcaat
     5281 gtcttagaga gcgcaggttg actcaaatta agggtttcag ccgcgcgccc caaagttcct
     5341 tgttgtgcga cagctacgaa tgtatgaagg tggcgcaaac ggatgcgctg actaaacaga
     5401 ctattttttt ccataagcga tgttaaaaac gaagcggtgt cgctgacaag tgaagttgtt
     5461 tgattatgat aacttgattg caaaatatta ttaacaatta aagcaattat gttacagcaa
     5521 aatggataat attgatgttt tcgcggcgag atcacagttt gtaaattctt cccgcaagag
     5581 tgaatgcggt tacctacact ccagattact gaccactgga ggcagacact atggcgaaca
     5641 gcatcacggc ggatgagatt cgggaacagt tttcgcaggc aatgtcagcc atgtaccagc
     5701 aagaagttcc gcaatatggc acgctgctgg aactggtagc tgatgtgaat ctggctgtgc
     5761 tggaaaacaa tcctcaactg cacgaaaaaa tggtaaatgc agacgagctg gcgcgactga
     5821 atgttgaacg tcatggggcg attcgcgttg ggactgcaca agagcttgct actcttcggc
     5881 ggatgtttgc cattatgggg atgtacccgg tgagctatta cgatctctcg caggcagggg
     5941 tgccggt
//
LOCUS       DAMZET010000218         5901 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.218, whole genome shotgun sequence.
ACCESSION   DAMZET010000218 DAMZET010000000
VERSION     DAMZET010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.218"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..232
                     /locus_tag="POB34_004317"
     CDS             <1..232
                     /locus_tag="POB34_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HDI5921164.1"
                     /translation="ALGAVKQATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQR
                     VNKLELNYDNFMEEFTAILHRQAFTVSESNKG"
     gene            236..664
                     /gene="tolR"
                     /locus_tag="POB34_004318"
     CDS             236..664
                     /gene="tolR"
                     /locus_tag="POB34_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HDI5921165.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            729..1994
                     /gene="tolA"
                     /locus_tag="POB34_004319"
     CDS             729..1994
                     /gene="tolA"
                     /locus_tag="POB34_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDI5921166.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            2127..3419
                     /gene="tolB"
                     /locus_tag="POB34_004320"
     CDS             2127..3419
                     /gene="tolB"
                     /locus_tag="POB34_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HDI5921167.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            3454..3975
                     /gene="pal"
                     /locus_tag="POB34_004321"
     CDS             3454..3975
                     /gene="pal"
                     /locus_tag="POB34_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HDI5921168.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            3985..4776
                     /gene="cpoB"
                     /locus_tag="POB34_004322"
     CDS             3985..4776
                     /gene="cpoB"
                     /locus_tag="POB34_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HDI5921169.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            4941..5016
                     /locus_tag="POB34_004323"
     tRNA            4941..5016
                     /locus_tag="POB34_004323"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4974..4976,aa:Lys,seq:ttt)
     gene            5152..5227
                     /locus_tag="POB34_004324"
     tRNA            5152..5227
                     /locus_tag="POB34_004324"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5185..5187,aa:Val,seq:tac)
     gene            5230..5305
                     /locus_tag="POB34_004325"
     tRNA            5230..5305
                     /locus_tag="POB34_004325"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5263..5265,aa:Lys,seq:ttt)
     gene            5455..5530
                     /locus_tag="POB34_004326"
     tRNA            5455..5530
                     /locus_tag="POB34_004326"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5488..5490,aa:Val,seq:tac)
     gene            5534..5609
                     /locus_tag="POB34_004327"
     tRNA            5534..5609
                     /locus_tag="POB34_004327"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5567..5569,aa:Lys,seq:ttt)
     gene            5756..5831
                     /locus_tag="POB34_004328"
     tRNA            5756..5831
                     /locus_tag="POB34_004328"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5789..5791,aa:Lys,seq:ttt)
BASE COUNT         1598 a         1397 c         1628 g         1278 t
ORIGIN      
        1 cgccctcggg gcggtaaaac aagcaacact gcaaatggtt gcgcccggta tcgcagaagc
       61 gttgattgcg actgcaattg gtctgttcgc cgcaattccg gcggtaatgg cttataaccg
      121 cctcaaccag cgcgtaaaca aactggaact gaattacgac aactttatgg aagagtttac
      181 cgcgattctg caccgccagg cgtttaccgt tagcgagagc aacaaggggt aagccatggc
      241 cagagcgcgt ggacgaggtc gtcgcgatct caagtccgaa atcaacattg taccgttgct
      301 ggacgtactg ctggtgctgt tgctgatctt tatggcgaca gcgcccatca tcacccagag
      361 cgtggaggtc gatctgccag acgctactga atcacaggcg gtgagcagta acgataatcc
      421 gccagtgatt gttgaagtgt ctggtattgg tcagtacacc gtggtggttg agaaagatcg
      481 cctggagcgt ttaccaccag agcaggtggt ggcggaagtg tccagccgtt tcaaggccaa
      541 cccgaaaacg gtctttctga tcggtggcgc aaaagatgtg ccttacgatg aaataattaa
      601 agcactgaac ttgttacata gtgcgggtgt gaaatcggtt ggtttaatga cgcagcctat
      661 ctaaacatct gcgtttccct tgcttgaaag agagcgggta acaggcgaac agtttttgga
      721 aaccgagagt gtcaaaggca accgaacaaa acgacaagct caagcgggcg ataattattt
      781 cagcagtgct gcatgtcatc ttatttgcgg cgctgatctg gagttcgttc gatgagaata
      841 tagaagcttc agccggaggc ggcggtggtt cgtctatcga cgctgtcatg gttgattcag
      901 gtgcggtagt tgagcagtac aaacgcatgc aaagccagga atcaagcgcg aagcgttctg
      961 atgaacagcg caagatgaag gaacagcagg ctgctgaaga actgcgtgag aaacaagcgg
     1021 ctgaacagga acgcctgaag caacttgaga aagagcggtt agcggctcag gagcagaaaa
     1081 agcaggctga agaagccgca aaacaggccg agttaaagca gaagcaagct gaagaggcgg
     1141 cagcgaaagc ggcggcagat gctaaagcga aggccgaagc agatgctaaa gctgcggaag
     1201 aagcagcgaa gaaagcggct gcagacgcaa agaaaaaagc agaagcagaa gccgccaaag
     1261 ccgcagccga agcgcagaaa aaagccgagg cagccgctgc ggcactgaag aagaaagcgg
     1321 aagcggcaga agcagctgca gctgaagcaa gaaagaaagc ggcaactgaa gctgctgaaa
     1381 aagccaaagc agaagctgag aagaaagcgg ctgctgaaaa ggctgcagct gataagaaag
     1441 cggcagcaga gaaagctgca gccgacaaaa aagcagcaga aaaagcggct gctgaaaagg
     1501 cagcagctga taagaaagca gcggcagaaa aagccgccgc agacaaaaaa gcggcagcgg
     1561 caaaagctgc agctgaaaaa gccgctgcag caaaagcggc cgcagaggca gatgatattt
     1621 tcggtgagct aagctctggt aagaatgcac cgaaaacggg gggaggggcg aaagggaaca
     1681 atgcttcgcc tgccgggagt ggtaatacta aaaacaatgg cgcatcaggg gccgatatca
     1741 ataactatgc cgggcagatt aaatctgcta tcgaaagtaa gttctatgac gcatcgtcct
     1801 atgcaggcaa aacctgtacg ctgcgcataa aactggcacc cgatggtatg ttactggata
     1861 tcaaacctga aggtggcgat cccgcacttt gtcaggctgc gttggcagca gctaaacttg
     1921 cgaagatccc gaaaccacca agccaggcag tatatgaagt gttcaaaaac gcgccattgg
     1981 acttcaaacc gtaatcgcga tgttgactgt tcggacggtc aacatcaggc accggttgcc
     2041 acggggttct ggtagttttg tgtattttag tttgttaaca ttctgctaaa ttatcgtggg
     2101 ccatcggtcc agataaggga gatatgatga agcaggcatt acgagtagca tttggttttc
     2161 tcatactgtg ggcatcagtt ctgcatgctg aagtccgcat tgtgatcgac agcggtgtag
     2221 attccggtcg tcctattggt gttgttcctt tccagtgggc ggggcctggt gcggcacctg
     2281 aagatattgg cggcatcgtt gctgctgact tgcgtaacag cggtaaattt aatccgttag
     2341 atcgcgctcg tctgccacag cagccgggta gtgcgcagga agtacaacca gctgcatggt
     2401 ccgcactggg cattgacgct gtagttgtcg gtcaggtcac tccgaatccg gatggttctt
     2461 acaatgttgc ttatcaactt gttgacactg gcggcgcacc gggtactgta cttgctcaga
     2521 actcgtacaa agtgaacaag cagtggctgc gttatgctgg tcataccgcc agtgatgaag
     2581 tgtttgaaaa actgaccggc attaaaggtg cgttccgtac tcgtattgcc tacgttgttc
     2641 agaccaacgg cggtcagttc ccgtatgaac tgcgcgtatc tgactatgac ggttacaacc
     2701 agtttgtcgt tcaccgttca ccgcagccgc tgatgtctcc ggcgtggtca ccagacggtt
     2761 ctaaactggc ttatgtgacc ttcgaaagcg gtcgttccgc gctggttatt cagacgctgg
     2821 caaatggcgc tgtacgtcag gtggcttcat tcccgcgtca caacggtgca cctgcattct
     2881 cgccagacgg cagcaaactg gcattcgcct tgtcgaaaac cggtagtctg aacctgtacg
     2941 taatggattt ggcttctggt cagatccgcc aggtgactga tggtcgcagt aacaataccg
     3001 aaccgacctg gttcccggat agccagaacc tggcatttac ttctgaccag gccggtcgtc
     3061 cgcaggttta taaagtgaat atcaacggcg gtgcgccaca acgtattacc tgggaaggtt
     3121 cgcagaacca ggatgcggat gtcagcagcg acggtaaatt tatggtaatg gtcagctcca
     3181 atggtgggca gcagcacatt gccaaacaag atctggcaac gggaggcgta caagttctgt
     3241 cgtccacgtt cctggatgaa acgccaagtc tggcacctaa cggcactatg gtaatctaca
     3301 gctcttctca ggggatggga tccgtgctga atttggtttc tacagatggg cgtttcaaag
     3361 cgcgtcttcc ggcaactgat ggacaggtca aattccctgc ctggtcgccg tatctgtgat
     3421 aataattaat tgaatagtaa aggaatcatt gaaatgcaac tgaacaaagt gctgaaaggg
     3481 ctgatgattg ctctgcctgt tatggcaatt gcggcatgtt cttccaacaa gaacgccagc
     3541 aatgacggca gcgaaggcat gctgggtgcc ggcactggta tggatgcgaa cggcggcaac
     3601 ggcaacatgt cttccgaaga gcaggctcgt ctgcaaatgc aacagctgca gcagaacaac
     3661 atcgtttact tcgatctgga caagtacgat atccgttctg acttcgctca aatgctggat
     3721 gcacatgcaa acttcctgcg tagcaacccg tcttacaaag tcaccgtaga aggtcacgcg
     3781 gacgaacgtg gtactccgga atacaacatc tccctaggtg aacgtcgtgc gaacgccgtt
     3841 aagatgtacc tgcagggtaa aggcgtttct gcagaccaga tctccatcgt ttcttacggt
     3901 aaagaaaaac ctgcagtact gggtcatgac gaagcggcat actccaaaaa ccgtcgtgcg
     3961 gtactggttt actaagagaa ttgcatgagc agtaacttca gacatcaact attgagtctg
     4021 tcgttactgg ttggtatagc ggccccctgg gccgcttttg ctcaggcacc aatcagtagt
     4081 gtcggctcag gctcggtcga agaccgcgtc actcaacttg agcgtatttc taatgctcac
     4141 agccagcttt taacccaact ccagcaacaa ctttctgata atcaatccga tattgattcc
     4201 ctgcgtggtc agattcagga aaatcagtat caactgaatc aggtcgtgga gcggcaaaag
     4261 cagatcctgt tgcagatcga cagcctcagc agcggtggtg cagcggcgca atcaaccagc
     4321 agcgatcaaa gcggtgcggc ggcatcaacg acgccgacag ctgatgctgg tactgcgaat
     4381 gctggcgcgc cggtgaaaag cggcgatgca aacacggatt acaatgcagc tattgcgctg
     4441 gtgcaggata aatcccgcca ggatgacgca atggtggcat ttcagaattt catcaaaaat
     4501 taccctgatt caacttacct gccaaacgcc aattattggc tgggtcagtt aaactacaac
     4561 aagggtaaaa aagatgatgc ggcgtactat tttgcttcgg tagtgaaaaa ctatccgaag
     4621 tcaccaaagg ctgctgatgc gatgtttaaa gtcggcgtca tcatgcagga caaaggtgac
     4681 accgcaaaag cgaaagccgt ataccagcag gttatcagta aataccctgg tactgatggc
     4741 gctaaacagg cacaaaaacg tctgaacgcg atgtaatgca taacacacga ccagaagtcg
     4801 cattatttct ggtcgtgtcg tgcgaatcat aagcagttga gtgatctgca tcgaaatttt
     4861 tgttgcgctc aagtctgaaa tcagtaatat atgccgccgt tgccacggga tatcaaacaa
     4921 accgaaagca acgaaaaagt gggtcgttag ctcagttggt agagcagttg acttttaatc
     4981 aattggtcgc aggttcgaat cctgcacgac ccaccaatcg ctaaggtgga agcggtagta
     5041 aaacgtgaag gataacgttg catcagcaac ggcccgaagg gcgagacgaa gtcgagtcat
     5101 cctgcacgac ccaccactaa catagttagt tgtagtatcc agcgtagtat tgggtgatta
     5161 gctcagctgg gagagcacct cccttacaag gagggggtcg gcggttcgat cccgtcatca
     5221 cccaccaccg ggtcgttagc tcagttggta gagcagttga cttttaatca attggtcgca
     5281 ggttcgaatc ctgcacgacc caccagtttt agcatcgaag acagatgtta agcgtgtagg
     5341 ataacgttgc gtcagcaacg gcccgtaggg cgagcgaagc gagtcatcct gcacgaccca
     5401 ccactaatta cggtgggttc ggtggaagta gtttgtagta tccagcgcag tatcgggtga
     5461 ttagctcagc tgggagagca cctcccttac aaggaggggg tcggcggttc gatcccgtca
     5521 tcacccacca ctcgggtcgt tagctcagtt ggtagagcag ttgactttta atcaattggt
     5581 cgcaggttcg aatcctgcac gacccaccaa ttttaacatc aaactcagat gttaagcgtg
     5641 aaggataacg ttgcgccagc aacggcccgt agggcaagcg aagcgagtca tcctgcacga
     5701 cacaccaatc ttaaagattg gccccgagta gaaatctttc aggtaacacc cgtatgggtc
     5761 gttagctcag ttggtagagc agttgacttt taatcaattg gtcgcaggtt cgaatcctgc
     5821 acgacccacc atcctgaatg attaaagcag cataatcccg caaggggtcg ttagctcagt
     5881 tggtagagca gttgactttt a
//
LOCUS       DAMZET010000219         5814 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.219, whole genome shotgun sequence.
ACCESSION   DAMZET010000219 DAMZET010000000
VERSION     DAMZET010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.219"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(129..1049)
                     /locus_tag="POB34_004329"
     CDS             complement(129..1049)
                     /locus_tag="POB34_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI5921170.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1187..2086
                     /gene="pgrR"
                     /locus_tag="POB34_004330"
     CDS             1187..2086
                     /gene="pgrR"
                     /locus_tag="POB34_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HDI5921171.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            2423..4036
                     /gene="mppA"
                     /locus_tag="POB34_004331"
     CDS             2423..4036
                     /gene="mppA"
                     /locus_tag="POB34_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HDI5921172.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(4087..5118)
                     /gene="ynaI"
                     /locus_tag="POB34_004332"
     CDS             complement(4087..5118)
                     /gene="ynaI"
                     /locus_tag="POB34_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HDI5921173.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            5362..5619
                     /locus_tag="POB34_004333"
     CDS             5362..5619
                     /locus_tag="POB34_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HDI5921174.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(5669..>5814)
                     /locus_tag="POB34_004334"
     CDS             complement(5669..>5814)
                     /locus_tag="POB34_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="with UspC and UspD is involved in resistance to UV
                     irradiation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HDI5921175.1"
                     /translation="TVGRTGISAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDE
                     EDD"
BASE COUNT         1580 a         1277 c         1365 g         1592 t
ORIGIN      
        1 gattaatcac atggcgggct atctgaccgc cagcaccaag aatcagtacc tttttcatga
       61 aaattatccc agattattgg cagcaatatg ccaggccata tcactattgc ggatttgaat
      121 atgtttcatt acagagtttc ctcaaagaac ggagctaata ctgaaattgc ttcagccaca
      181 taggctttgc cgtcataaag atccatatgg tttgcgcctt caacaatgtg atagcgttta
      241 tcctggcttg atgctcgatc gtacaggtcg tcactcatcc atttgctccc tgcctggctg
      301 cccgccacaa tctgcatcgg ctgagtcagg tacacttccg ccatatggta agcatcatag
      361 gtaataatct ggttaaggct gcgcaaagta gcgtaacccg gtgctgttgg atactgcgcg
      421 cgaggggtgt ggtaatactc ccaggcctga cgcagttctt cgttcggcgc atcggactcc
      481 ttcattggtg ccagtggcat aacggcatat tctccgctgc taatatcgct ggttcgggcg
      541 tttgaacccg cttcaacgta tggcagggca tcaatagatt tcacattgtt ttcccaacca
      601 ttacggaaca tcgaaccaat attgaccgca ctaacggtac cgatggcctt gatgcggcga
      661 tcctgaattg cagcattggc tgtatatcct gcaccggcac aaattcccat cgcaccaatt
      721 cgggtattgt cgacataaga aagcgttgtc aggtaatcaa tcacggcact gatgtcttca
      781 gtacgaatgt atgggttttc taactgacgc ggctcgccgc cactttcacc ttgataagat
      841 gcgtcataag caatagtgac aaatcccttt tccgccagtt ttttggcata ggttccggcc
      901 gtttgttctt taacgccccc acctgggtga gataccacaa ttgcctgata ctgacgggtt
      961 tcatcaaatt ttggagggaa atagatcact gcagacaaag agatggttgg attattgctg
     1021 ttagtgaagc tgactttatt attcatcatt ccgttcctca tggagttgtc ggttcgtttt
     1081 aacggttggt gatatcacta tagatattga tcattaagtt gattagaccc aaaatcatga
     1141 ttagacctat catttaaatg attaatagat tgcatggaga tactgaatga agcgtgaaga
     1201 aatcgctgat ctgatggcgt ttgtcgtcgt tgcagaggag cgtagcttca ctcgtgcagc
     1261 agcccgcctg agcatggcgc agtcagcttt aagccagata gtgcgtcgta tagaagaacg
     1321 attgggattg cggcttctga cgcgaaccac gcgcagcgtt gttccaactg aagcgggcga
     1381 gcatcttttg tctgttcttg gccctatgtt gcatgacata gattcagccc tggcatccct
     1441 gagcgagctg cagaaccgcc catccgggac aatacgtatt actactgtag aacatgcagc
     1501 aaaaacgata ttgttaccag caatgcgcac attcctgaaa tcgcatcctg aaattgatat
     1561 tcagctcacc attgattatg gtttgaccga tgtcgtttct gaacgttttg atgcaggcgt
     1621 ccgtctgggt ggggagatgg ataaagatat gatcgccatt cgaatcgggc cagatatacc
     1681 aatggctatt gttggctcac cggattattt ttctcgccga agtgttccaa cgtcagtgtc
     1741 acaattaata gatcatcagg caattaattt gtatcttccc acatcgggta cagcaaatcg
     1801 ctggagatta atacgcggtg gacgtgaagt tcgtgttcgc atggaaggtc agcttttact
     1861 gaatacgata gacctgatca ttgatgctgc aattgatggg catggattgg cgtatctacc
     1921 ttatgatcag gttgagcggg ctattaaaga aaaaaaactg atacgtgttt tggataaatt
     1981 cacaccagat ttacccggtt atcacctgta ctatccacac cgtcgacatg ctggctcggc
     2041 attctcatta tttatagata ggctgaagta taaaggtgct gtttagcact acttgctgat
     2101 acattaattt aatttctctc ttaacgtatt ctcagttcct ttcaacgttt tggttatttt
     2161 ttattcttcg tacaatggcg acagatgctg attatgatac cgaaaactgg tttgaacgtg
     2221 cgaagcctga acgtagtgtt cggtgttcta tgtgctttac cgcattttgg cgtcgattat
     2281 ttacactaaa tctgatttaa tatattgata cataaaacat tttatgcttc ctgtgtcact
     2341 tttttgatgg aagttgtttg catttcttta aggcgaaaca aataattacg catcaatttt
     2401 aatgtcggtt agagggaaac ttatgaagca ctctgtttca gtcacgtgtt gtgcgctgtt
     2461 ggtcagcagc atttctcttt cgtatgctgc agaagttccg agcggcacag tactggcaga
     2521 gaagcaggag ctggtgcgcc acattaaaga tgagcctgcg tcgctggatc ccgctaaagc
     2581 cgtgggcctg ccagagattc aggtcattcg cgatctgttt gaaggactgg tgaatcagaa
     2641 cgagaaaggg gagattgtcc ccggcgttgc gactcagtgg aaaagtaatg acaaccgtat
     2701 ctggactttt accctgcgcg ataacgcaaa atgggcggat ggcacaccgg taacggcgca
     2761 agattttgtc tacagctggc aacgtctggt ggacccgaaa actctatcgc catttgcatg
     2821 gtttgccgcg ctggcgggaa tcaacaacgc acaggcgatt attgatggta aagctacgcc
     2881 tgaccagctt ggcgtcaccg cagttgatgc ccatactttg aaaattcagc ttgataaacc
     2941 gttgccgtgg tttgtgaatt taaccgctaa ctttgccttc ttcccggtgc aaaaagccaa
     3001 cgtagaaagc ggtaaagagt ggacgaaacc cggaaatctg attggcaatg gcgcttatgt
     3061 tcttaaagag cgcgtagtca atgaaaaact ggtcgtggta ccgaataccc attattggga
     3121 taacgccaaa acggtactgc aaaaagtgac cttcctgcca attaatcagg aatccgcagc
     3181 cactaagcgt tacctcgcgg gggatattga tatcaccgaa tccttcccga aaaatatgta
     3241 tcagaagctg ttgaaggata ttccggggca ggtttatacg ccgccgcagc tcgggaccta
     3301 ttattatgcg tttaacacgc aaaaagggcc gacggcagat cagcgcgttc gtctggcatt
     3361 aagtatgacg atagatcgcc gcctgatgac cgaaaaagta ttagggacgg gcgaaaagcc
     3421 agcgtggcat tttacaccag atgttaccgc cggatttacg ccggaacctt cgccgtttga
     3481 acaaatgagt caggaagaac tgaatgcgca ggcaaaaact ttgttgagcg cagctggtta
     3541 tggtccgcaa aaaccgctga agctgacgct tttgtataac acttcagaaa accatcaaaa
     3601 aattgcgatt gctgtagcat cgatgtggaa aaagaacctt ggcgtagatg ttaaattgca
     3661 aaatcaggaa tggaaaacct atatcgatag ccgtaacacc ggcaattttg atgttatccg
     3721 cgcctcatgg gtgggggatt ataatgaacc ctccactttc ctgacattat taacgtcaac
     3781 gcattcagga aatatttcac gctttaacaa tccggcatat gacaaagttc tggcccaggc
     3841 atcgacggaa aataccgtta aagcgcgtaa tgccgattac aacgcggcag aaaaaatcct
     3901 catggagcaa gcaccgattg caccaattta tcaatatacc aatggacgat taatcaaacc
     3961 gtggctgaaa ggttatccca ttaataaccc tgaagatgtg gcgtacagtc ggactatgta
     4021 tattgtgaag cattgatgtg atgggaactg gcgttaccct tgtggataac gccagtgatg
     4081 tctgatttag cgaccctgtt ccggcggtgt aatgttatcc atatacagcg tctggctggg
     4141 gaaggcaaag tccgcgccgt gtgactgtac aatatcgata atcttcaaat aaacgtcttg
     4201 ctgtgcagca agccattcag cccataccgt ggttttggta aagcaataaa ccataatatt
     4261 caatgacgag tcggcaaact ggttgaaata aaccagtaag gtttgtcgct ggtcgatggc
     4321 cgggtgattt ttcagcatct cacgtacggc ttcgacaata acgcccactt ttgccgcatc
     4381 ctcataacgt aaaccaatgg tcgtggtaat gcggcggttg gtcattcgtc ctgggttttc
     4441 tacgctgatc gacgaaaaca gcgagttcgg tacgtacaat ggacgattat caaaggtcgt
     4501 aattttggta attcgccagc caatttccgc tactgtacct tcgatatttc tgtccggtga
     4561 acggatccag tcgccaatac tgaaaggacg gtcgaaatag agcatgatcc cggaaaagaa
     4621 attactcaga atatctttac cggccatacc gacagccaga ccaccaatac caccaaaggt
     4681 cagcaagcca gaaaggctca tgccgaaatg ttcgccataa agcagaacaa gcaccacaat
     4741 aatggtgatt ttgataatgc gcgacataat ccgcgcactg gtgatatcgc gacctttttt
     4801 aatctgctgt ttttcaaact gattaatcag caaaaatagc ttaatcgtca gaataaccgc
     4861 aatcagggac gtacagataa aatcgataac gcctggggtg ataaatttga gtttatagtt
     4921 ttctataaca taattaataa tgctaccaac agcactgata attatggtgt agattaaaaa
     4981 ttgcaccgca tggaataaaa aaccttttct tttacgattt ccgcggcgaa accaaaagct
     5041 catcagaatc aatgctgcgc agctaccgaa aataatgacc agattaagcg cattatttgt
     5101 aaacagttca gcgatcattg ttttatcagg ctcctccaga taattgtcgt catgccggaa
     5161 acccctggcg gggctatttt accgcgacaa ttcattcaga tcatcaatag tcagggaagg
     5221 aagtagcaac attagctaac taatcaatgc gttgattgta aatccagcta agaggtgagg
     5281 ttttcagagc agacaacggt gaaatgtcat ggtattgtta cgtttaggta acaagaaatt
     5341 tgtctgcaca aggattacat catgattatg gcgaaactga agtcagcgaa agggaagaaa
     5401 tttctctttg gtttgttggc ggttttcatt attgcggcgt cggttgtgac tcgcgcgact
     5461 atcggcggcg ttatagaaca gtacaatatt ccgctgtctg agtggacgac atcaatgtat
     5521 gtgattcagt catcgatgat ttttgtttat agcctggtct ttactgtgtt gctggcaatc
     5581 ccgttgggaa tttatttcct tggcggcgaa gagcagtaag taaaaaatag gcccgataac
     5641 tcgggccttg tcagttattg aagagtcgtt aatcgtcttc ttcgtcatcc agttcaacgg
     5701 gtgtctgata ctggtcaggt ttaatgacca acagatcgca gcgaagatga tcaatcacct
     5761 gttccgccgt gttgccgagg aatgctgctg aaataccggt gcgtcctacc gtgc
//
LOCUS       DAMZET010000220         5758 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.220, whole genome shotgun sequence.
ACCESSION   DAMZET010000220 DAMZET010000000
VERSION     DAMZET010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.220"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..684)
                     /locus_tag="POB34_004335"
     CDS             complement(<1..684)
                     /locus_tag="POB34_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921176.1"
                     /translation="MATAGILTIRAANTPEQHVQAVYKAEQDINSTKNENSKANKTKG
                     ENGFAIVTSGLASSGNDVYENYMALAFDSVDDVNVRRSADVTSYPVENGATVSDHVQI
                     KNNKFSLKGRITETPIKSDPGLLKSAGVNGNRRSLAIDYLNQIMDSRQPFLLVTENKT
                     FENVVLTGIEYTEEASESLVFDLSFEQIRLVSYGTVNTVAIKTQPSKNIGANMKKRVN
                     TERSSSEGED"
     gene            complement(718..2553)
                     /locus_tag="POB34_004336"
     CDS             complement(718..2553)
                     /locus_tag="POB34_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921177.1"
                     /translation="MLEINTSKIKNTVTFSVDKDSLKKAKDSITGLKEFAENIKPAKL
                     RFDIVTKGYKKAQSEVDKIAQQKAKTEKQNQRAQAAAARAQARQQAQIAARKEKADLK
                     LLDVGSSISAMHRLSVAEQYKAIAQAREIALQYERGAISLARMNSQMKRLQQQQRKIN
                     GNRKAQLAPVKGGGSGNAATMGALLFGGATAAAGVMAVSKASEFVSSSFANADTQGEL
                     IQRAKLGGVDVNQMYNITEWAYKNGVDSMMGDQGARKYLDQMKDVRERAAKSYSEAEL
                     VKDKKTGKSGKWKGGDNAINELLNIGVINKSDLKKFADNPAGLISKAVNGMMKKGFSD
                     SQIGQRLEDLGDDLMLTSKYWQRSVKDVQESINQQKASGKWLTETQQDSIVKFRELNR
                     QLSQLSDARQVAFVDGFMKSLDPKVTEEFLKNLSNLTPYFTKLGEATGSLFEAIMKIV
                     NWFNRNDDKTDAIQKNLGDAPPLSNDGMKQNLSNLVPDKYKGAGTATTTPDNSHSLFN
                     TIKGLLFDDNSSVSDVKMSVSEAPITNLKQGALNNLAMTTPAYNLSPVFNLNPTFEVV
                     TEVPLTINSDTSRLSDYVDFTARASRDSFLKSLTLTSLSGQSNGG"
     gene            complement(2573..2773)
                     /locus_tag="POB34_004337"
     CDS             complement(2573..2773)
                     /locus_tag="POB34_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000452041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921178.1"
                     /translation="MEVILKSKNGVHVHLDANDSRGLLNLKYLCSLLDVPYEGVKARM
                     FRLNESIDQALHHFLSKEGGKK"
     gene            complement(2864..3892)
                     /locus_tag="POB34_004338"
     CDS             complement(2864..3892)
                     /locus_tag="POB34_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HDI5921179.1"
                     /translation="MILGNDYVDLAPLFSAHSTRNYLLSTLDFTDPVGVSSHKVAVSQ
                     LVESNESLFNKETSRFSSEHNVTKREQGKEWLIEIPYFLREDVIRPSDVQGKRKSGTD
                     FQETLTDIYAEYIAKHHVAYQRTKESVLAASLFSGKTYTPKTDDVLIEWSKLFNVSPM
                     KTTVNASSTDTTKIFKEFDQIATNIFEITQSQASAVERIVVFCKPDAFSAIRFSAGMA
                     NAFQYVSPLEEGNVVYQRRDLLPGVTAFTIPGTNIDVVKLVDPLHLAHMTADAVAVPK
                     FAKGSNVYQNIYGAASSTFELINAAPAEVYSYSYESSRGDAVNVVTENSQMVVNHGVG
                     FSVQITVK"
     gene            complement(3911..4279)
                     /locus_tag="POB34_004339"
     CDS             complement(3911..4279)
                     /locus_tag="POB34_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921180.1"
                     /translation="MIIDLDTLFPGRNRKTFTDIVSYCTDPFASVESKVFASLPADVE
                     CGDLITSTGAKYESGDDIYVVMSEFVTAGENKPVDVLRSNAGLVCIKADALNAVSEAA
                     KTALIKKGFQLEGFNTVFTS"
     gene            complement(4276..4488)
                     /locus_tag="POB34_004340"
     CDS             complement(4276..4488)
                     /locus_tag="POB34_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921181.1"
                     /translation="MLFLNDKEQIIKYKDEMLKINPQITEMVAKYAGCSVEEVERCVE
                     KYFSPSSPSTPSLNELIKLKAKEITK"
     gene            complement(4839..5177)
                     /locus_tag="POB34_004341"
     CDS             complement(4839..5177)
                     /locus_tag="POB34_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921182.1"
                     /translation="MNKNTYDTIYSLINYYEDDYLLPLNRAELEAYKENTPAALNEAF
                     KHWDLAVNAFEHLSKRVEMLCKRENAYLTADQIWELSNWIEDIESDVRYVGDGLVELA
                     QRLGATITEE"
     gene            complement(5179..5589)
                     /locus_tag="POB34_004342"
     CDS             complement(5179..5589)
                     /locus_tag="POB34_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921183.1"
                     /translation="MAKRKNNVVKKIGDSASLLSKPKSILRRKDFKELVTLTKQNSAP
                     GEWKTEIIEHSPSVPCGDEYIALQEILSSTPGVFWKPKKRKEYIVDSSDLRKYQILGF
                     EDYNHYVGYLATNGLNNLVPEFQILDNADHYGDF"
BASE COUNT         1625 a         1186 c         1043 g         1904 t
ORIGIN      
        1 gtcttcacct tcactacttg atctttcagt gttaacacgt tttttcatat tagccccgat
       61 gttcttagat ggctgtgttt ttatagctac agtgtttaca gtgccatacg aaacgagcct
      121 gatctgctca aagctaagat caaataccag agattcgctt gcctcttctg tgtattcgat
      181 gcctgttaaa acaacgttct caaaagtttt attttctgta acaagaagaa aaggctgtct
      241 actgtccata atctgattca gataatcaat agccaatgat cttctgttcc cgttcacccc
      301 tgcactcttt aacaagccag gatcgctttt aatcggtgtt tcagtgatac gccctttaag
      361 agaaaattta ttatttttaa tttgaacgtg atcagacaca gtagcaccat tttctacggg
      421 gtagcttgtg acatctgccg aacgtctcac gttcacatcg tcaacactat caaaggccag
      481 tgccatataa ttttcataaa cgtcattgcc agaagatgcc agcccactgg taacaatagc
      541 aaatccattc tctccttttg ttttattagc tttgctattt tcgttttttg tagaatttat
      601 atcttgttct gctttgtaaa cagcctgaac gtgttgttct ggagtatttg ctgcacgtat
      661 ggttaaaatc ccggcggtcg ccataataat aatttccttg taatgttatg gcgggaatta
      721 cccgccattg gattgtcctg ataagctggt taatgttaat gatttcaaga agctatcccg
      781 acttgctctc gctgtaaaat ccacataatc gcttaatctt gatgtgtcag aattaatagt
      841 aagaggaacc tctgttacca cttcaaatgt aggattcaga ttaaacacag gagataaatt
      901 ataagccgga gttgtcattg caagattatt taaagcaccc tgtttgagat tggttatcgg
      961 tgcttcgctg actgacattt ttacatctga cacagaagaa ttatcatcga aaagcaatcc
     1021 cttaatcgtg ttaaagagag aatgactatt atcaggtgta gttgttgcag tgccagcgcc
     1081 tttatattta tcaggaacga gattagaaag attctgtttc attccgtcat tgcttaatgg
     1141 cggtgcatca cctaaatttt tctgaatagc atctgtctta tcatcattac gattaaacca
     1201 gtttacaatc ttcataatgg cctcaaagag acttcctgta gcttctccca gcttcgtaaa
     1261 atacggagtg agattagaaa ggtttttcaa aaactcttcc gttacttttg gatcaagaga
     1321 tttcataaag ccgtctacga atgctacttg tcttgcatca gaaagctgtg aaagctgtct
     1381 gtttagttct ctgaatttaa caatagagtc ttgctgtgtt tctgttagcc attttccaga
     1441 ggctttttgc tggttaatac tttcttgtac atctttaacg gaacgttgcc aatattttga
     1501 tgtaagcatc aaatcatcgc ccaggtcttc taaacgttgt ccaatttgag aatctgaaaa
     1561 cccttttttc atcattccat taacagcctt agaaattaat ccggctggat tatcagcaaa
     1621 ttttttaagg tcgcttttgt taataactcc gatgtttaga agttcattaa tagcgttatc
     1681 accgcctttc catttccctg attttccggt tttcttgtct ttcaccaatt cagcttcact
     1741 ataggatttt gcggcacgtt ccctaacatc tttcatctga tctaggtatt ttctagcccc
     1801 ttgatcaccc atcatagagt caacgccgtt tttatacgcc cattctgtaa tattatacat
     1861 ctggttaacg tctacaccac ccagctttgc acgttggatt agctcccctt gggtgtctgc
     1921 gttagcgaaa ctgctactaa caaactccga cgctttacta acagccatca cgccagcagc
     1981 agccgtagcg cctccaaata acagagcgcc catagtggcg gcattccctg atcctcctcc
     2041 cttaacgggg gctaattgag cttttctgtt gccattaatc ttacgttgtt gctgttgtag
     2101 gcgtttcatc tggctattca tacgtgccag actaatagcg cctctttcat attggagggc
     2161 aatttctcta gcctgtgcaa tagctttgta ttgttccgct acagagagac ggtgcattgc
     2221 actaatgctt gatccaacgt ctaacagttt taagtctgct ttctccttgc gtgctgctat
     2281 ttgtgcctgt tgccttgcct gtgctcttgc tgcggcagct tgtgctcttt gattctgctt
     2341 ttctgtttta gctttctgtt gagcaatctt gtctacttca ctttgtgctt tcttatagcc
     2401 tttagtgaca atatcaaatc ttaatttagc tggcttgata ttttctgcga attcctttaa
     2461 gcctgtaata gagtctttcg cttttttcaa gctgtcttta tctacactaa atgtaactgt
     2521 gttcttaatt ttagaagtgt taatttctaa catttaaaat cccccttatt aatcattttt
     2581 taccaccttc tttgcttaag aaatggtgta gagcttgatc aatactctcg tttaatctga
     2641 acatacgggc ttttactcct tcatatggaa catctaggag agaacagaga tatttcagat
     2701 ttaacaagcc tctagaatca ttagcatcta aatgtacgtg tacaccattt tttgatttca
     2761 aaatcacttc catattttaa tcacctctca aaatttaaaa aaataaataa aaaaaaaggc
     2821 gaagcgtgaa tacacgccac gcctctatta cacgttaaaa atattattta acagtgattt
     2881 gaacggagaa gccaacgccg tggtttacaa ccatctggct attctctgta acaacgttaa
     2941 cagcatcacc acgagaagat tcatagctat agctatacac ctcagcagga gcggcattaa
     3001 tcagttcaaa ggtgctggaa gcagccccgt agatgttctg atatacgtta gagcctttag
     3061 caaacttcgg aaccgctaca gcgtctgcgg tcatatgtgc cagatgtagc ggatctacca
     3121 gtttaacaac atcgatgtta gttccaggaa tagtgaacgc tgtcactcct ggcagcaggt
     3181 cacgacgctg atacacaacg ttcccctctt ccagtgggct tacatactgg aatgcgtttg
     3241 ccataccagc agagaatcga attgcagaga aggcgtcagg tttacagaaa acaacaattc
     3301 tttctaccgc tgacgcctgg ctttgtgtga tttcaaaaat gttagtagca atctgatcaa
     3361 attctttaaa aatcttcgtg gtgtccgtgc tggaagcgtt caccgtggtt ttcattggtg
     3421 atacgttaaa cagcttgctc cactcaatta acacatcatc agttttaggc gtgtaagttt
     3481 tgccagagaa caaagaagcc gccagcacgc tctctttagt gcgctgatac gctacgtggt
     3541 gtttagcgat atattccgca taaatatcag tgagtgtttc ctgaaaatca gtgccagact
     3601 tacgtttgcc ttgaacatca gaaggacgga tcacatcttc acgaagaaaa tacgggattt
     3661 cgataagcca ttctttacct tgttcacgct tagtaacatt gtgttcagaa ctaaaacggc
     3721 ttgtctcttt gttaaagaga gattcgttgc tttcgacaag ctgggaaaca gctactttat
     3781 gagaactaac gcccaccgga tcagtgaagt caagggtaga cagtagatag tttctagtgc
     3841 tatgcgcaga gaacaaagga gctaaatcaa cgtaatcatt acctagaatc atttattatt
     3901 attcctttta ttaagaagtg aaaaccgtat taaagccttc aagctgaaag ccttttttaa
     3961 ttaatgctgt ttttgctgct tcacttacag cattcagggc atcagctttg atgcatacaa
     4021 gcccagcatt gctacgtagc acatctacag gcttgttttc tccagccgtt acaaattcac
     4081 tcataacaac atagatatca tctcctgatt catatttagc gccagtgctg gtgatcaagt
     4141 cgccgcactc aacatcagca ggaagagaag caaaaacctt gctttctaca gaggcaaacg
     4201 gatcagtaca ataacttaca atatctgtga aagtctttcg atttcgacct ggaaacagag
     4261 tgtctaaatc aataatcatt tagtaatctc cttagctttt aatttgatta attcattaag
     4321 ggaaggtgta gacggagaag aaggagaaaa atatttttca acacatcttt ctacttcctc
     4381 gacagaacac ccagcgtatt tagctaccat ttcggtaatt tgtggattaa ttttcagcat
     4441 ttcatcttta tacttgatga tttgctcttt atcatttaag aacaacattt atataaacct
     4501 tgttaccttg tttaaagtaa gaaatacaac ttttaagttg cataacgggc aaaataaaag
     4561 gcagcattta aagccgcctt ttattaactc atacctatgt aattacccta tgttatgcat
     4621 actccaagat gtatgcataa caaaggaaaa aagaggtaat aatgacacag tatttactta
     4681 aggaggagaa gcggggagcg gagattttac tcccctttaa tgacaggaag aatttttaat
     4741 atcgaaccaa taaaggaaaa ataatcaccc ctatataatt atattaacat acaatagatg
     4801 aaatgtcaag atattttaca acttttttgt tgcttttttt attcttctgt gatagtagcg
     4861 cctaagcgtt gagctagttc aacaagtcca tctcctacat agcgaacatc agattcaata
     4921 tcttctatcc agtttgatag ctcccatatc tgatcggctg tcaggtatgc gttttcacgc
     4981 ttacagagca tctctacacg cttagagagg tgctcaaagg cattcacagc taaatcccag
     5041 tgtttaaacg cctcatttag tgccgctggc gtgttttctt tgtatgcttc aagttcagcg
     5101 cggtttaaag gcaggaggta atcatcctca taataattaa ttagtgagta aattgtgtca
     5161 tatgtgtttt tgttcatatt aaaagtctcc atagtgatca gcattatcta aaatttggaa
     5221 ttcaggaact aaattattta ggccatttgt ggcgagataa ccgacgtagt ggttatagtc
     5281 ttcaaatcct aaaatctggt atttgcgtaa atcagagcta tcaacgatat actcttttct
     5341 ttttttaggt ttccagaaca cgccaggtgt tgaagataag atttcttgta atgcgatata
     5401 ttcatctcca caaggcacag agggagaatg ttctatgatc tctgttttcc attcgccagg
     5461 ggcgctattt tgcttggtga gcgtaactag ctctttaaag tctttacgtc ttagaataga
     5521 tttaggttta cttagaagag aagctgaatc accgattttc ttaacaacgt tgttttttct
     5581 ttttgccatt tatttttcct tttcttttaa aggaaaacac cccgaaatat gcttctaacg
     5641 tgagaaagaa caataaccaa tacatagcta ctatcataat aatgttttcc ttttatattt
     5701 taattacata gagagatttt agagagtgga atagatgaat tctatttctt tagaaagt
//
LOCUS       DAMZET010000221         5745 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.221, whole genome shotgun sequence.
ACCESSION   DAMZET010000221 DAMZET010000000
VERSION     DAMZET010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.221"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..812)
                     /gene="hcaR"
                     /locus_tag="POB34_004343"
     CDS             complement(<1..812)
                     /gene="hcaR"
                     /locus_tag="POB34_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HDI5921184.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDYPLAHEKEITAAQLDGVNFVSTDPVYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMA"
     gene            948..2309
                     /locus_tag="POB34_004344"
     CDS             948..2309
                     /locus_tag="POB34_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HDI5921185.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKIPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            2306..2824
                     /locus_tag="POB34_004345"
     CDS             2306..2824
                     /locus_tag="POB34_004345"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HDI5921186.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            2824..3144
                     /locus_tag="POB34_004346"
     CDS             2824..3144
                     /locus_tag="POB34_004346"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HDI5921187.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            3141..3953
                     /gene="hcaB"
                     /locus_tag="POB34_004347"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             3141..3953
                     /gene="hcaB"
                     /locus_tag="POB34_004347"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HDI5921188.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            3963..5165
                     /locus_tag="POB34_004348"
     CDS             3963..5165
                     /locus_tag="POB34_004348"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HDI5921189.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            5262..5684
                     /locus_tag="POB34_004349"
     CDS             5262..5684
                     /locus_tag="POB34_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDI5921190.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
BASE COUNT         1361 a         1478 c         1540 g         1366 t
ORIGIN      
        1 cacgccatca gtaaagcaat ggaaggcaca tttccggcaa tgggacgaaa aacaacctgt
       61 ccggtattaa aattattcat ataaccgggt atcaaagtga cgcccagccc catgcccacc
      121 agattcatgg tcaccagaat attcgttgcc acctggacga tatttggctg gctgttttct
      181 tgcgcaaacc acgctttaac gatcggcgca agcgaaccgg aatacaccgg atcggtactg
      241 acgaaattca cgccatccag ttgcgcggcg gtgatctctt tttcatgtgc taaagggtaa
      301 tcaaccggta acacaaccac taatggttcg tcaaaaagct ccagataatc aatctccggg
      361 ctataaacag gatggcgcat taagccgacg tcgagttcac cacggcgaat tttttcctcc
      421 tgttgcgtgg tgattaaact caccagctca atcaaggtgt ctggctgtct gagacgaaac
      481 atcggtaata cttttggcag taaattcact tccgccgatg gcacaaagcc aatggttaat
      541 tgtctgtctt cctgaacaat tttccgcgcc cgtaatttgg cattttccgc ttgttcgagg
      601 attgccagcg cgtcctggag aaaacattct cccgccgccg tcaacgcgac tttgcgctta
      661 tccctcacca gtaacggaac accgacacag ttttcaagat cgcggatctg gctgcttaac
      721 gaaggctgtg aggtatgcag tttttccgcc gcacgggtaa agttcagtgc ctgcgccact
      781 gcgacgaaat agcgtaaatg ccgtagttcc atcaccttcc ccttgttatc gaaaaaacgt
      841 ctcagctggt agaaattaac tatttcacat attagcaacc aaccagcaac atccttatgg
      901 cacaaaaata gaaggtcaat acatcttatc tttcaggatt aaaaaatatg accacaccct
      961 cagatttgaa catttaccaa ctgattgaca cccaaaatgg tcgggtcact ccgcgtattt
     1021 ataccgaccc ggatatttac caactggagc ttgagcgtat tttcggtcgt tgctggctat
     1081 ttctcgccca cgaaagccag atcccaaaac ccggtgattt ctttaacacc tacatgggag
     1141 aagatgcggt tgtcgtagtg cgtcagaaag acggcagcat caaggcgttt ctcaaccaat
     1201 gccgccaccg ggccatgcgt gtgagttatg cagattgcgg caacactcgc gcctttacct
     1261 gcccgtatca cggctggtct tatggcatta acggcgagtt gatcgatgta ccgctggaac
     1321 ctcgcgccta cccacaaggg ttgtgtaaat cccactgggg actaaacgaa gttccttgtg
     1381 tggagagtta taaagggctg atttttggca actgggatac cagcgcaccg ggcctgcgtg
     1441 attacctggg tgacattgcc tggtatctgg atggcatgct ggatcgtcgc gaaggcggca
     1501 ccgaaattgt cggcggcgta caaaagtggg tgatcaactg taactggaaa atcccggcag
     1561 agcagttcgc cagtgaccag tatcatgctc tgttcagcca tgcttctgcc gttcaggtat
     1621 taggggcgaa agatgatggc agcgataagc gcctcggtga tggacaaacc gcccgcccgg
     1681 tgtgggaaac cgccaaagat gcgctgcaat ttggtcagga cggtcacggt agcggtttct
     1741 tctttactga aaaaccggat gctaatgtct gggtcgatgg cgcagtttca agctattacc
     1801 gcgaaaccta tgccgaagca gaacaacgtt taggtgaagt tcgcgccctg cgcctggcgg
     1861 gtcataacaa tatattcccc acgctttcat ggctaaacgg cactgccaca ctccgcgtct
     1921 ggcatccgcg cggccctgat caagttgaag tgtgggcgtt ctgtattact gacaaagccg
     1981 cctccgatga agttaaagcc gcttttgaaa acagcgccac tcgtgctttt ggtcctgctg
     2041 gttttctcga gcaggatgac tcggagaact ggtgtgaaat ccagaaattg ctgaaaggtc
     2101 accgcgcccg caacagcaaa ctgtgtctgg aaatggggct tggtcaggaa aagcgtcgcg
     2161 acgacggcat tccaggcatt actaactata tcttctcaga aacggccgct cgtggaatgt
     2221 accaacgctg ggccgatctt ctgagtagcg aaagctggca ggaagtgctc gataaaaccg
     2281 ccgcttacca gcaggaggtg atgaaatgag tgcgcaagtt tcactagagt tacatcaccg
     2341 cattagccag tttctctttc acgaagccag cttactggac gactggaaat ttcgtgactg
     2401 gctggcgcag ctcgacgaag agattcgtta caccatgcgc accacagtta acgcgcaaac
     2461 acgcgaccgc cgcaaaggcg tccagccacc gacaacctgg atttttaatg acaccaaaga
     2521 ccagctggag cggcgaatcg cccgtctgga aacgggcatg gcatgggcag aagagccgcc
     2581 gtcacgcacc cgccacttaa tcagcaactg ccagataagc gaaaccgaca tcccaaacgt
     2641 atttgctgtt cgggtgaatt atctgcttta tcgggcacaa aaagagcgcg atgaaacgtt
     2701 ctatgtcggg actcgttttg acaaagttcg ccgtctggaa gatgacaact ggcgcttgct
     2761 ggaacgggat atcgtcctgg atcaagcggt aatcacttcc cataacctga gtgtactgtt
     2821 ctgatgaatc gaatttatgc gtgtcccgtt gcggatgtgc cggagggtga ggctctccgg
     2881 atcgatacct cgcccgtcat cgccctgttc aacgttggcg gcgagtttta tgccattaac
     2941 gatcgttgca gccatggtaa tgcgtcaatg tcagaagggt atctggaaga tgacgccacg
     3001 gtggagtgcc cgctacacgc cgccagtttt tgcctgaaaa cggggaaagc gttatgcctg
     3061 cccgccaccg atccgctcac cacttatcca gtacacgttg aaggtggtga cattttcatc
     3121 gacttaccgg aggcgcagcc atgagcgatc tgcataacga gtccattttt attaccggcg
     3181 gcggatcggg attagggctg gcgctggtcg agcgatttat cgaagaaggc gcgcaggttg
     3241 ccacgctgga actgtcggcg gcaaaagtcg ccagtctgcg tcagcgattt ggcgaacata
     3301 ttctggcggt ggaaggtaac gtgacctgtt atgccgatta tcaacgcgcg gtcgatcaga
     3361 tcctgactcg ttccggcaag ctggattgtt ttatcggcaa tgcaggcatc tgggatcaca
     3421 atgcctcact ggttaatact cccgcagaga cgctcgaaac cggcttccac gagctgttta
     3481 acgtcaatgt tctcggttac ctgctgggcg caaaagcctg cgctccggcg ttaatcgcca
     3541 gtgaaggcag catgattttc acactgtcaa atgccgcctg gtatcctggc ggcggtggcc
     3601 cgctgtacac cgccagtaaa catgccgcaa ccggacttat tcgccaactg gcttatgaac
     3661 tggcaccgaa agtgcgggtg aatggcgtcg gcccgtgtgg tatggccagc gacctgcgcg
     3721 gcccacaggc gctcgggcaa agtgaaacct cgataatgca gtctctgacg ccggagaaaa
     3781 ttgccgccat tttaccgctg caatttttcc cgcaaccggc ggattttacg ggtccgtatg
     3841 tgatgttggc atcgcggcgc aataatcgcg cattaagcgg tgtgatgatc aacgctgatg
     3901 cgggtttagc gattcgcggc attcgccacg tagcggctgg gctggatctt taaggaagca
     3961 cgatgaaaga aaaaaccatc attattgtcg gtggcgggca agcggcggca atggctgcgg
     4021 cctcgctacg ccagcaaggg ttcaccggtg agctgcatct gttttccgat gagcgacatc
     4081 ttccttatga acgacctcca ctctcgaaat ccatgttgct ggaagattcc ccgcagttac
     4141 agcaggtgtt acccgctaac tggtggcagg aaaacaatgt tcatctgcat tccggtgtaa
     4201 ccatcaaaac gctgggtcgc gacacacgag agttagtgtt aaccaacggc gaaagctggc
     4261 actgggatca gctttttata gcaaccggcg cggcagctcg accgctgccg ttgcttgatg
     4321 cactgggaga acgctgcttt accctacgcc atgccggtga tgccgccaga ctgcgagaag
     4381 ttctgcagcc cgaacggtca gtcgtgattg tcggtgccgg aactattggt ctggaactgg
     4441 ctgccagcgc cacgcagcgc agatgtaagg tgacagtgat tgaactggcg gcaaccgtca
     4501 tgggccgtaa tgcaccaccg cccgtgcaac gctatctttt acagcgccac cagcaggctg
     4561 gtgtgcgcat tctgctcaat aatgccattg aacatgtggt cgatggtgaa aaagtagaac
     4621 tgacgctgca aagtggggaa acgcttcagg ctgatgtggt gatttacggt attggtatca
     4681 gcgccaacga gcaactggct cgcgaggcca accttgatac tgccaatggc attgtcattg
     4741 atgaggcttg ccgcacctgc gatcccgcga tctttgccgg tggcgatgtg gcaatcactc
     4801 gtcttgataa tggtgcacta caccgctgcg aaagctggga aaacgccaat aaccaggcgc
     4861 aaattgccgc tgccgcaatg ttagggctac cgctaccgct actgccgccg ccgtggttct
     4921 ggagcgatca gtacagtgat aacttacagt ttattggcga tatgcgtggc gatgactggc
     4981 tttgtcgtgg caacccggaa actcagaagg cgatttggtt taatctgcaa aacggcgtgc
     5041 ttatcggtgc ggtcacgctg aatcaggggc gtgagattcg cccaattcgc aaatggatcc
     5101 agagcggcaa aacgtttgat gcgaaactgc tgatagatga gaacatcgcg cttaaatcac
     5161 tgtaaccagg ataattagcg aatatctcaa tgcctggggc gtggcgaggt gcaagagtgt
     5221 gtattacgtt taaatcacat tatcttgcaa agggattggt tatgaacaca ctacgttatt
     5281 ttgattttgg agctgcccgc cccgttttgt tattaattgc ccgtatcgcc gtggtcttaa
     5341 ttttcattat ttttggtttt cccaaaatga tgggctttga cggtacggtc caatatatgg
     5401 cctcgttggg cgcgccgatg ccgatgctgg cagcgattat tgcggtagtt atggaagtgc
     5461 ccgccgcgat attaatcgtg cttggctttt tcacccgtcc gctggcggtg ctgtttattt
     5521 tctacacgct gggtacggcg gtgattggtc accattactg ggatatgacc ggcgatgcgg
     5581 ttgggccaaa tatgattaat ttctggaaga atgtcagtat cgctggcgca ttcctgctgc
     5641 tggcaattac cgggccgggg gcaatttctc tcgatcggcg ttaggaaaga tgccggatgc
     5701 ggcgtgaacg ccttatccgg catttaataa attacagcca ctcta
//
LOCUS       DAMZET010000222         5738 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.222, whole genome shotgun sequence.
ACCESSION   DAMZET010000222 DAMZET010000000
VERSION     DAMZET010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.222"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..325
                     /locus_tag="POB34_004350"
     CDS             <1..325
                     /locus_tag="POB34_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HDI5921191.1"
                     /translation="TELARSVFPADHAELQWRQSRGLTALLMALLAISLSRVKPRQGR
                     FSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPGLMLMGLLMLVARDFSLL
                     QKFSR"
     gene            322..1401
                     /gene="lptG"
                     /locus_tag="POB34_004351"
     CDS             322..1401
                     /gene="lptG"
                     /locus_tag="POB34_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDI5921192.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFTAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(1437..2609)
                     /locus_tag="POB34_004352"
     CDS             complement(1437..2609)
                     /locus_tag="POB34_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDI5921193.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(2609..2857)
                     /locus_tag="POB34_004353"
     CDS             complement(2609..2857)
                     /locus_tag="POB34_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDI5921194.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(2889..3803)
                     /locus_tag="POB34_004354"
     CDS             complement(2889..3803)
                     /locus_tag="POB34_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDI5921195.1"
                     /translation="MNQTVAVTGATGFIGKYIINNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSKLVAGASAVVHCAGQVRGHKEEIFTHCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVPGGYDW
                     QHVRQLAADARCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(3800..5530)
                     /locus_tag="POB34_004355"
     CDS             complement(3800..5530)
                     /locus_tag="POB34_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HDI5921196.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHDNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPG
                     DRCVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQASQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIELLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLHVQESLA"
BASE COUNT         1405 a         1463 c         1490 g         1380 t
ORIGIN      
        1 cacggagctg gcgcgctccg tctttcctgc cgatcatgcc gaactgcaat ggcgacaaag
       61 ccgtggtctg acagcattgt tgatggcgct gctggccatt tcattaagtc gggtaaaacc
      121 gcggcaaggg cgattttcaa cgttattgcc actgacgttg ctgtttattg ccatttttta
      181 tggcggcgac gtctgccgta cgctggtggc taacggcgcg attcccctca ttcctggttt
      241 gtggttagtg cccggactca tgctaatggg cctgctgatg ctggtcgcac gcgacttctc
      301 tttgctgcag aaattttccc gatgaatgtt ttcagtcgct atttaatccg tcatctcttt
      361 ctcggttttg ccgccgccgc agggctattg ctgccgcttt ttaccacctt caacttgatt
      421 aacgaactgg atgatgtcag cccgggcggt tatcgctgga ctcaggcggt gctggtggtg
      481 ctaatgacct taccgcgcac actggtcgaa ctttcgccgt ttatcgcgtt attgggtggt
      541 attgttggac tggggcagtt atcgaaaaac agtgagctta ccgccattcg cagcacgggg
      601 ttttctatct tccgtattgc actggtggcg ctggttgccg ggatattgtg gactgtttcg
      661 ttaggcgcga tagatgagtg ggtggcgtcg ccattgcagc aacaggcgtt gcaaatcaaa
      721 tcgaccgcca ccgcgttggg ggaggacgat gacattaccg gcaatatgct gtgggccagg
      781 cgtggtaatg aatttgtgac ggtgaaatcg ctgaacgagc agggccagcc ggtgggcgtg
      841 gagatatttc attatcgcga cgatctctcg cttgaatcct acatttatgc acgcagtgcc
      901 accattaaag acgacaaaac gtgggtcctg catggtgtga atcataaaaa atggctgaat
      961 ggcaaagaaa cgctggaaac attagataat cttgcctggc aatcggcctt caccagtatg
     1021 aatcttgaag agttatcaat gccggggaat actttttctg tccgtcagct taatcattac
     1081 atccattatt tgcaggaaac cgggcaaccc agcagcgaat accgccttgc actgtgggaa
     1141 aaactggggc aacccatcct gaccctggcg atgattttgc tggctgtgcc gtttaccttt
     1201 actgccccgc gttcgcctgg gatgggtagc cgtctcgctg taggcgtcat cgtgggctta
     1261 ctcacctgga tcagctatca aatcatggtt aatctgggat tgctatttgc gttaagcgca
     1321 cctgttaccg cgctcggttt accggtagcg tttgtgttgg tggcgttgag cctggtgtat
     1381 tggtatgaca gacaacatta aacccgccgt ggcgcaaaca ccacggcggg ctcacattac
     1441 tgcttcagag tagcaaatgc ctgaatgatg gcatcgattt gctcacgact gtgggcagca
     1501 tttacgctac agcgcagcag cgtgatgcct gcaggcgcgg caggcggcag gataagattc
     1561 acatacacgc ccagcgagat aagctcgcgc cagatgcgta aaccttcctc tttcgaaccg
     1621 attattactg gcactaccgg gctaatatgt gaacttaact cataacccag tttttgcaga
     1681 ccgtcataca gatggttggc gttatccatc agcttttgcc gcagctctgg ttgtgtggca
     1741 atagttctta acgacgagcg cacggtcgca atgcaggacg gagaaggaga ggcggtaaaa
     1801 atatacgggc ggctgccgta gcgcagcact tccatcgctt ccgaaccgac agcaaaaccg
     1861 ccaatcgagg ccagactttt actgaaagta ccaacgataa tatccacatc atcttcaacg
     1921 ccgacagctt cagccagtcc gcgccccgtt gcgcctaaca cgccaaacga atgcgcttca
     1981 tcgacaatca aataaccacc gagacgacgt ttgatatcaa caatttccgc cagcggcgca
     2041 acgtcaccga gcatgctgta gatgccctca acgatgataa tcgcctcttt cgcccgttca
     2101 ccgaggcgaa ccatgcgccg ttccagatct ttagcatcgt tatggcggaa acgaataatt
     2161 tccgcgccac cgagagaaca ggcgtcataa atactggcgt ggctgtcggc atccagtaac
     2221 accacggcgt tatggtcggc aagtgcgctg atgacgccta aattagcggt ataaccggtt
     2281 gaaaaaacga tagcggtagg gcgattgaaa aactcggcaa tctctttctc taacgccagg
     2341 tgcggcgcat aactgccgtt tgccatacga gaaccggtgg tgccggtgcc ttgggccgct
     2401 aacgctgcct gaccttcgct gatggcatca tgattaaagg ttaaaccgag ataattattg
     2461 gtcccggcca gaatcacttt tttattgccg atccggcctt cggtggcgga atacacttca
     2521 tcaatacagg tgccaaacgg tgttaacccg ttgtcactaa attgcagtcg ctcgccagca
     2581 aggcgcgcat atttatcgta tagccccatt atttattctc cagccatgga agtagagttt
     2641 ccattaattg cgcaggggtt ttgacatcca gcagaatatt aataggaatg gaaatatcga
     2701 atctgtcttc cagcatcatt aagagatcca tcactttgat cgactccagg ccaagatcgt
     2761 tgacgagatc gctgtcgggc ttaatttcca caccgttttc gaccaaatcc tgtaaacagg
     2821 aaaggatgta atccattact atttcacgat ttaccataga aacgttaaca cacctttaaa
     2881 caagacaatc aaaatagccc gttgcgtaat gcgtgttcca ggctaatccc gggaaaccaa
     2941 ttaatatctt cagaaatacg gttattactt gccgaccagt cggcgtgggt taattcacga
     3001 attttcgagc gggtcagcat aggttcttta cctgccaggc gactcaacgc ggtactgata
     3061 tccgcaaggc aggtgagtac cggcagagga atgccaacca ttcgcacgga accacaacgg
     3121 gcgtcggcgg caagctgtcg tacgtgttgc cagtcatagc cgccggggac gccatcgcat
     3181 aattcatagg tttgcgtctg tacagtttcg gcgcttaacc actgacccac tgcttgcgcg
     3241 aaatcggtga cgtgcaggaa agagagctgt gtatctggcg caccaagtcg tggcagcagg
     3301 ccgcgcagca tccagtcaaa cagcggtttt aactctttat cgccgggacc atacacggct
     3361 gtcgggcgaa aaacgcccag cgtaatttcg tcagccattg cagtcagacg ttgttcggcg
     3421 acgtgtttgg aatttgcgta ccaggagagc tcgggatggc gcgccgccag cgaagagata
     3481 aacagaaaac gttggcaaaa gccgctctct tttgctgctt gcatcaggcg caggctgccg
     3541 tcaacgttac agtgggtaaa aatctcttct ttgtgcccgc gcacttgccc ggcgcaatgg
     3601 accaccgcgc tggctccggc aaccagcttg ctaagtgaat gagtatcttc cagcgaaccg
     3661 cgcacccagg tgagattatc attgacgtga gcgcgggcag tacgcgtcaa tgcgcgaacg
     3721 tgaaagccgc gggcgagcag gttattgata atatatttac cgataaaccc ggtagcgccc
     3781 gtcaccgcga cagtttgatt catgccaggg attcctgcac atgaagactg gcagcataag
     3841 ccttctgata acgttttttc gcttccgcac gggcaggctt gccggaggac gttcggggaa
     3901 tactgtgggg cggcaacagc tcgatagccg cggtcacgcc aaattcgctt tggatccgtg
     3961 ccgccagcgc gtggataagc tgcccgcgac gttcttcgtc gctgatccga cactggatct
     4021 gcaaaatgat tttttcctgg gcggtaacaa aagcaatcgc atcgccagaa tgaatttccg
     4081 gttcttgttc cgcaatatat tcaatatcct gcggccagat attacggcca cgaataataa
     4141 tcagatcttt aatgcgtccg gtgacataca gataaccgtc cagcagataa ccgaggtcgc
     4201 cggtgtctaa ccagcccgtc gcggcaatct cgtcttgcga agcctggtcg ccaaagtaac
     4261 cgctcatcag actgggaccg gagatgcaaa tatggcctac cacacgttcc gcgaccggca
     4321 tacctgcttc attgcggatt tcaataccat gttccggcaa cgctttgccg cagttgacga
     4381 aagtcgatac ggcgcgtgtc tctgcacccg gcgcgacggc tttaccctga tattcgagga
     4441 tgtcgcgatc cacttcgtta accacaaccc cggaggcttc atcagagaag ctgacagcca
     4501 gcgcattttc tgccagtccg tagcacggca tgaaagtttt attgtcaaag ttaacctgac
     4561 ggaaacattc agcgaattga tggagttgtt ctgcggagat gggttctgca ccaataccag
     4621 cgacgcgcca gcaggaaaga tccagttcag cgagatcttt ttcattcacg cggcgctggc
     4681 acaattcata gccaaacggc ggcgcaacgg aaacggtgcc gcgattttta ctgatcaatt
     4741 taagccattg cagaggacgc atggcaaaat cctgagtgcg caaataatct actgaaagct
     4801 gcgtggcgac gggggtcagg agaaagccga ccagtcccat atcatggtag aaaggcagcc
     4861 aggagacgca gcggtcgcca gggcgtaatt taatgccgtc gtggcttata gcacgtagat
     4921 tagccatcac ttcgcgatgg gtgataatga cgccacgggg aaaacgggtg ctgccggagg
     4981 tgtactggag gtaggcgata tcgttcggaa ctggacgctg gagcgcaaca tcggcttccg
     5041 gtaatgcctt aaaccaggcg tggcttaaaa catgtaattc ggggttgtca tgcgtcgcgg
     5101 cattgaccag tggcaaccac tcatcaccag tgataatggc tgcgggctgg caacttgcca
     5161 gtaaaccctg caatttggcg ctccaggaat cccgctgacc aacgcccatt ggaatcgcca
     5221 acgggacggc gactaagccg gcatactggc aggcaaaaaa agcctctacg aacccgctac
     5281 ttgtttcggc aatcagtgcc acgcgatcgc cttttttcag gttcagcgat aacaaccgct
     5341 tcgcaccagc ttcggcacgc gtttttaatg tctgatattc cagttgatct tcaagttggc
     5401 aacgtctgtc ataaaaattc attccggcgc tactcagagc ggcgtagtcc aaagcatcaa
     5461 ccagcgttgg aaaatcggca tagcgcatag gtagggaatg cgtaaagatt ttattagaca
     5521 tatacaccac gaaaaaattg gatatcaaaa ataaaatgac aataatgcgg tggattatat
     5581 aggcataaaa gataaatgag atagtatttt catcggtgtg aaaatgaaca ttgatttaaa
     5641 taatgagttt aagttttttg tttttattgt gaatgatatt taatttatga atatattaag
     5701 aagaagcctt gtccggtcgc cttaaatcga caaaaggc
//
LOCUS       DAMZET010000223         5721 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.223, whole genome shotgun sequence.
ACCESSION   DAMZET010000223 DAMZET010000000
VERSION     DAMZET010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.223"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(499..712)
                     /locus_tag="POB34_004356"
                     /pseudo
     CDS             complement(499..712)
                     /locus_tag="POB34_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            951..1835
                     /locus_tag="POB34_004357"
     CDS             951..1835
                     /locus_tag="POB34_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HDI5921197.1"
                     /translation="MSKLSSEMKALAKKAGGSFKTVNDRIHITKRFSEHLRALNIQIQ
                     RVEQIRVRHIECYIEERLEQDIGLRTLQNDMAALQAVLRQAGRRQVVEHPRLTNKALG
                     VSGASRNGTRRAITPEHYQQVMEKARAEDAGLAAALEIARLMGLRSQEAVQSSLSLKT
                     WLKAIERGENRLKVVFGTKGGRPRHTTALDTGAVRKALEKALIVAEQHNGRLIDKPDL
                     KMALESWHKQVIKVGLKGEFSPHSLRYAWAQDAIRHYLAQGFSEKESLALTATDLGHG
                     DGRGRWVKQVYGYRWKRE"
     gene            complement(3230..4492)
                     /locus_tag="POB34_004358"
                     /pseudo
     CDS             complement(3230..4492)
                     /locus_tag="POB34_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055676.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            complement(4654..4729)
                     /locus_tag="POB34_004359"
     tRNA            complement(4654..4729)
                     /locus_tag="POB34_004359"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4694..4696),aa:Asn,seq:gtt)
     gene            complement(4830..5627)
                     /gene="mtfA"
                     /locus_tag="POB34_004360"
     CDS             complement(4830..5627)
                     /gene="mtfA"
                     /locus_tag="POB34_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HDI5921198.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
BASE COUNT         1496 a         1373 c         1465 g         1387 t
ORIGIN      
        1 gaaaactgat tgcggatgcg ctggaaacat caccggaaat tatcttgccg tccggataca
       61 tcgatgatcg gggcaaccgt accggaagac tcatgcaaaa acgccgatcc tgatggctga
      121 atcgtcaggc gcactgttac agtaaaacca gactctcccg gcgcgctgcc tgcttttgct
      181 ggcagcgcgc cgggagaaga caagtgaagc gcggcagttt ttccgtcacc aggtaagcaa
      241 acaatctttt aaacccggaa acagtttttc cttcctgcac cagaaataat ggcgcagtct
      301 cccgcctgca acaaactgcc tccaggcagt tcctttgtcg cttcacgtct ggaagaagcg
      361 ctaggcatcc tttaccggcg ttgatctctg aaaacgccgg ggatgcaaat atacctgcat
      421 ctgagactga cgtacatata caccatcact gcaccagata cccggcttta cgggctgaca
      481 ctctggcttt gccgggtttt atcttctgct gacaaccagc gccttacatt gacgggctgc
      541 ggcctgcaaa gacagcaggc tttgctgccg cgtgaccgtc accctgcacc agaaacgcaa
      601 cgtttacacc cagcaccaga caaggcgtca ctttgatcgc gccagcgaca gggcaaagtg
      661 acgctgcaca actcaccgcc gcaagccgcg ccagtcccgg cctgcgccat atttccgccg
      721 tctgacatgc cctttggggc tgctgtcagt tcagagtaaa ggagaaaaag atcgataccg
      781 aggggggcac gacgccccgg agacacccct gcttcggcag gcagtctgta ccgcgtacag
      841 cctgaaactc cctaaacctc aacaaactgc cactccggcg tgaaggtatc gttctggcgc
      901 agccaaaaag cacacgccgg agactgtttg tttttgttgc aggggaattc atgagcaaat
      961 taagcagtga aatgaaggcg ctggcgaaga aagcgggcgg cagctttaaa acagtgaatg
     1021 accgtatcca tattacaaaa cggttcagtg aacatttacg ggcgctgaat atccagatcc
     1081 agagggtgga gcagatcagg gttcgccata tcgaatgcta cattgaggaa cggctggagc
     1141 aggatattgg tttacgaacg ctgcaaaacg atatggcggc actgcaggcc gtgctgagac
     1201 aggctggacg caggcaggtg gtggagcatc cgcgcctgac caataaggcg ctgggagtgt
     1261 cgggcgcttc acgcaacggt acgcgccgcg ccattacgcc ggaacattac cagcaggtta
     1321 tggagaaagc ccgcgctgag gatgcggggc tggctgcggc gctggagatc gcgcgactga
     1381 tggggttacg ctcgcaggaa gcggtacaga gttcactgtc gctgaaaacg tggctgaaag
     1441 ccattgagcg cggggaaaac cggctgaaag tggtgtttgg caccaaaggt gggcgtcccc
     1501 gccatacgac agctctggat actggcgcag tcaggaaagc ccttgaaaaa gcgttaattg
     1561 tagcagaaca acacaatggc aggctgatcg acaagcctga tctcaaaatg gcgctggaga
     1621 gctggcacaa acaggtcatt aaggtggggc tgaaggggga attttcccct cactccctgc
     1681 gctatgcctg ggcgcaggat gcgatccgcc attatctggc gcagggattc agcgaaaagg
     1741 aatccctggc gctgacggcg acggatctgg gtcatggcga cgggcgcggg cgttgggtga
     1801 aacaggtgta tggctaccgc tggaagaggg aatgactgaa ttcatgaatg gctcaaaata
     1861 gtacgttaat gtgtaatgac agcgatcagt ctggcgcagg gaccggatga aataaaacga
     1921 caggatacct gtcgttgctg tatgacaaca taatgagaat attgttactt catggtgaat
     1981 aaatatcaat aactcagaat ttcctgcttc atcagcgcag gattactggg atattatggt
     2041 gattaccctg taatcactcg aaacgattcc tattgataac aggtgtttca atcattttta
     2101 ccaatactac agggctggga aaattttaaa atgtaaattc accggggcat cacattaatt
     2161 ctggcacagg gttatatctg aataattctc tctgtgtttt tccgcatcct gtgacatata
     2221 ttatatggtc tcaggatgcc tcatgatttt ataaaaatgc tgcgacggtt tttggcataa
     2281 taacctgctt catatgaatt aagaatcttc ggtgcttcat gttatttttt gcactttgtg
     2341 attgttatcc tgtggtgaaa atacagtctg gtgcagactg tcatggagca tttccaaata
     2401 aactggctgc gccagaaagg gaaagggtca taaaggttaa gggggcgata aggggaacag
     2461 cgcctgacct gaaagggcca aaaactaaag ggccaggtac tggcgcagca ctgaacaggt
     2521 agagcggcgg gacgctggcg cagatagcag attatccagg acgggtgcag attattttgt
     2581 ctctgtatag ctggtgcagt gataatgtgt atcaggaaag attgtccggt attttatggc
     2641 gcattttggg gatgtattaa gtggtaattc cgtggaaatg tcgattattg atagcttttc
     2701 accttccatg attaacatgt gaatcacgga aggtggctgc ctgttgaaaa cagatgcatt
     2761 gataggtttc gcctgaaatc attggacgcc aaataatttc aggcttatat ctgtctggtt
     2821 tctgaatatc tgatgcagtt cttacaggtt tcagagtata gggtcaggag atattctggt
     2881 gcagatattc tggagtatta atttgtattt tcgtgaaaat atcccgtatt gataggtttt
     2941 catcttctgt gatttgcagg caaatcacag aaggtagtta tctatcaata atgcatttat
     3001 taataggtaa tcacctgcaa atattctact ggactaattt ttggtattta cctattgata
     3061 tgtggtctat tgattggctt tcacccaaaa ttattcacca gtgaataatt ttgggtgaaa
     3121 gccaattaat aataacaaaa acagcgacaa tacctctcaa tacaaaaaaa cagatcccgc
     3181 tcaatcagga tctgcttttc cggttttaat gatttacgct atttctgatt caggctgctt
     3241 tatgctgccg cgcataaatg tacggcgcga catacccctc gcggcacaca tccagataat
     3301 ccgaccacca ctgcatcatg gcctttctgg cctccagatg ctccgcttta tgcacgtatg
     3361 ccgcccgcac gctgttacgc tcctggtggc tcatttgccg ctccaccgta tcgcgtgacc
     3421 agagatccga ctccaccagg gcactacagg ccattgccct gagtccatgc ccgcagattt
     3481 cagttttcgt gtcatacccc atcaggcgca gcgcccggtt ggttgtgttt tcgctcattg
     3541 gcttgtacgg attatgatcg ccgggaaaca ccagatccag atggccggaa agctcctgta
     3601 tctgtttcag aagggcaatc gcctggcggg agagcggtac gatatgcgga gtacgcattt
     3661 ttgcgccacg ccccgaaaac cgaactttgt cgatggcctc gcgggtggcg gggatggtcc
     3721 agattttgtg cctgaaattg atctcgctcc agcgggcgaa acgcagttcg ctggaacgga
     3781 tgaacaggtg cagtgtcagc accaccgcca gccgggttaa ctcccggccc tgctgatagt
     3841 caccgatgcg gccaagcagt tcggacaacc gctccagcgg taacgcgggg tagtgattct
     3901 ttacggggga ggagacaacg ccttccagat gctgcgccgg attgctttcg gtaagcccct
     3961 gctatacggc atagcgcatg atgttgctga gttgctgccg cgtgcgggac gcgacttcca
     4021 gaaagccctt atcctcaata accctcagca gggcagtgaa atcctgcgtt ttaagtttgg
     4081 caacgggcaa atgaccaacc gccgggaaga tatggttttc catactggcg agaatgcggg
     4141 cggcataatc agccgaccat tttttgttgg ttttgtgcca ggccagcgcc accgctttaa
     4201 agcacttttc aggggagcag gcggctttct ctgccatgcg ctgttgcgcc gggttgatat
     4261 tctgcgccag taacttacgg ataccgtcgc gctgctgcct ggcttctgcc agtgagacca
     4321 gcggatatgc gccaatactg actctggatt cttttccgtt gaaacgatat ttgagatacc
     4381 agatgcgtga accgcccgga ttgacgagca gatacagacc gtgagagccg gagagtttta
     4441 ccggacggga atagggtttt aacttgcgga ttttagaatc gtttaaagac atttgggggt
     4501 cactccagaa tcgaaccaaa ctgaccccag atctgaccac caaattatcc cgatgcggag
     4561 agaaaaccag atacgcaccg ggaaggattt tcacgctaac ttactgaatt gtaaacaaat
     4621 ataaacgtac agataagcat gaaaacttga atctggctcc tctgactgga ctcgaaccag
     4681 tgacatacgg attaacagtc cgccgttcta ccgactgaac tacagaggaa tcgtgtgaac
     4741 ggggcgcata ttacttagcg gtaccttgtc tgtcaacact aaatttcata agtcatttca
     4801 attggttaat taatctgcaa agttgttaat taatgaacat tcgtcgccga aaacgagtct
     4861 gtatcattag cgtgatgcag tctctgcaaa ggatcttgtt gataaaattg gcagaaacgt
     4921 tgccacaatg aagggaaacg aggagcaaaa agttctgggg cgctaaagaa atattcagaa
     4981 agtacggcaa aacattcagc aggatcactg gcagcataag catcaatgct cgccgcattc
     5041 tcgccaacca attcgatttc ttcctgaatg ttgttcattg cagcatgaag atcgtgttcc
     5101 cagccagcaa cctcacgcaa cggaataaag ggaactccgc tggcgcgatc gccgttacgg
     5161 gtgtccagct tatgagcgac ttcatgaata atcaggttaa aaccagaagc atcaaaagaa
     5221 tcttgtatat ccaaccagtt caaaacgata ggcccttgct gccagctctg acctgactga
     5281 acaatacgtt ggttatgcac cagaccgata tcgtcttccc attcatcatc gaccacaaat
     5341 ggcgcaggat aaattaagac ttcatgaaaa ccatccagcc attccagtcc taactccaga
     5401 acgggtaggc aaaatagaag tgctatccgg cagcttctta atgaatccag ttcaaagccc
     5461 tgtaaaggaa caagccgctt ttgctgtaaa aaacgttcgg caagagtgac taatttgctt
     5521 tgttcctgtt ccgtcagacc cgttaaaagg gggatcgata gtgcttcctg ccagggaagg
     5581 gcagtttgat gtgctgattc ttgtactttc cagggccact taatcatcgt tttgctcgca
     5641 aactcgtcac ttgaacaaaa ttgcacggac agggactgtt aaaatgccaa atttcctggc
     5701 atcatggcaa ccatctgaac g
//
LOCUS       DAMZET010000224         5604 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.224, whole genome shotgun sequence.
ACCESSION   DAMZET010000224 DAMZET010000000
VERSION     DAMZET010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.224"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..650)
                     /gene="tssI"
                     /locus_tag="POB34_004361"
     CDS             complement(<1..650)
                     /gene="tssI"
                     /locus_tag="POB34_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDI5921199.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRY"
     gene            complement(1387..2835)
                     /gene="cusS"
                     /locus_tag="POB34_004362"
     CDS             complement(1387..2835)
                     /gene="cusS"
                     /locus_tag="POB34_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HDI5921200.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLSVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
     gene            complement(2825..3508)
                     /gene="cusR"
                     /locus_tag="POB34_004363"
     CDS             complement(2825..3508)
                     /gene="cusR"
                     /locus_tag="POB34_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HDI5921201.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAMIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3665..5047
                     /gene="cusC"
                     /locus_tag="POB34_004364"
     CDS             3665..5047
                     /gene="cusC"
                     /locus_tag="POB34_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HDI5921202.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            5071..5403
                     /gene="cusF"
                     /locus_tag="POB34_004365"
     CDS             5071..5403
                     /gene="cusF"
                     /locus_tag="POB34_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HDI5921203.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGEV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            5419..>5604
                     /locus_tag="POB34_004366"
     CDS             5419..>5604
                     /locus_tag="POB34_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415106.1"
                     /note="with CusA, CusC and CusF is part of a cation efflux
                     system that mediates resistance to copper and silver;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDI5921204.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWVAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSP"
BASE COUNT         1505 a         1377 c         1375 g         1347 t
ORIGIN      
        1 ctgtagcgga actggctgat gcagagggtg ctcacctcgg tacgggtgtt cgggttccat
       61 gggatttcaa aagattcggg cagatggcgg actgtgtcgc acagcaccag gctctggtcg
      121 gtacttttgt aagcatgctc ctcataaaag aagatgcctt cctccgccgc catccggcac
      181 agaaaatcgt aatcagtctc accgtactgg acacaaaact cacgggaagg atgcggctcg
      241 ctgaacagcg gactccattc ggtcaccccg ttttcctgca acatcgtgcc gaggatgctt
      301 ttgatgtcct cgttctggaa gatacggaag ttctggcgca gaccggcacg ccacagcggc
      361 gggtgcacct tcatgctgta cagcatctgg tttttgtcgt tctccccgag ttcaaaccag
      421 gtcaccacgc ctttcacccg gcgctgtact tcatcgccct gccatatcgt caggtaggcc
      481 attttgtcca gcacctgcgc aaattcaagg gagagaaact gctggctgac cagggagaga
      541 tcgagggaaa agagcgaaga gagtgactgg ttcagatgaa aggaaaccac cgcaaaagca
      601 tccggcggca ggccgtccac ttccagtgtg aaacgtaatc cggttgacat agcaacctcc
      661 agtaaaatga ataatgatcc gtaccgtaca actgcggata atgtgataag cgggacgact
      721 tcagacgtta ttagggaagg aacagaaaat gctatggtta tgtgagaatt ttgtttaaat
      781 attactttac agaaacgggt gtctgttcag tcaaaaaaat aatcacaagc tcaacctctt
      841 gtttcttggc gttattcagg acaggtacgc atatcacaac gagtaacaaa aacgtaaagt
      901 taaaccaata ttcataatat atttttattc acagcaataa ataatccaat taacattatt
      961 aaaatgaatg attgcataag aaatatatgc taccgctcca ccctgataac atgatctgca
     1021 cattgcacat cttaacgttg ttaaatactg gctcaatagt ggatatttta aattaggaat
     1081 ttatcaaatt cacttgataa atagattgag tgaataatat aacaattaat gatgattaat
     1141 cgcgattaaa ttattcattg ctttatatga tagatggctt gtttataacg atgaaaattt
     1201 agaaagggca ttgtgatcta caattggaag aaacacagat ttaactaagt ttcatataca
     1261 aacatctcgt gaaactttga aaaataaaag ccttcgatat ttcatatgtt taataagttt
     1321 ttctctaatt tgttgatcaa caataccagg aggtgatgca ctacgccctg aatttgaact
     1381 taataatcac tctctctccg gcagcataat cacaaacctt gtcccccgcg catctgacgt
     1441 tactgcaacc gtgcctttat gcgcgacaac aatcgatttc actatcgcca gcccaatgcc
     1501 gctaccttcg ccttttcgct ggcgggaagg gtcaacgcgg tagaaacggt caaacaatcg
     1561 cggtaagtgc tcgggcgcaa tgggcgtacc ggggttttcg acggtaactt gcacctggtg
     1621 atcgaccgtc tggcagcgca ctacaattgc ctctccgggc ggcgtataac gcagggcatt
     1681 agaaagcagg ttgcttaacg cccgacgcag catcagcgga tcgcccgcaa cctgacattc
     1741 gtcgccaaca aatcgcaact ccacgccgcg atcttccgct aacgcctcga aaaaatcgaa
     1801 cactttgccg acttcatccg ccaggttgag cattttcttt tcggggatta gctggttgtt
     1861 atcggcctga gcgagaaaca gcatatcgct gaccattttc gccattcgcg tcagctcttc
     1921 gagattagag tagagcacat cttccagctc cttctggctg cgagactggc tgagggcgat
     1981 ttccgtttgc gtgatgagat tggtaatcgg cgtgcgaatt tcgtgagcga tatccgctga
     2041 gaaattggac tggcgggtaa agacatcctc aatacgctcg atcatatggt tgaacgacag
     2101 taccagctgt tccagctcaa tgggcacggt ctgcgggtca aggcgaacgt cgagatcttt
     2161 cgaggtgata ttctggattt gacggctgac gctgcggatc ggcgcgtgac ctttatgtac
     2221 cgccaacagt acgataaaga cgatcaggat gctgattacc gatgcggtca taataagttt
     2281 attcatcaaa tcatttatgt aatgaagatg aaaatcgatc gaaagcgcga tgtagagcgt
     2341 ataaatcggt ttgccgtcca ccaacgggcc aaccgacaag ttaatcatcc gccagttgct
     2401 gtgttccata tgcccgtgac cgtggcctgg catcataatc gtcgggccgg aaaggagata
     2461 cacctcgcca ccccgcgcgt ctttatcggg tatggcatca cgtgcaaact cgcggatatc
     2521 aggcgcaccg ggggagtgat acaccgtttt gccgtggcta tctgccaggg aaatcaacac
     2581 gttggaataa ccactgacga tatcttccag cgtcattaag cgtcgggctt gcgtttcgtc
     2641 agggtgatta agtacccgtt caagcgtggc gctaatctct tttaaatcat taatatcctg
     2701 ctcggcaaaa tgcactttta ctgagtggat catgatccat gcaaaggcga aaaacgccgc
     2761 gatggtggcc aggctgataa aaaaggtcag gcgggttgcc agcgaaaacg ggcgctgaaa
     2821 tggcttactg accatccggc acctcaagca tgtagcccac gccgcgcacg gtctgaatta
     2881 gcttcggctc aaagtcgttg tcgattttgc cgcgcagccg cttcaccgct acatcaatgg
     2941 cgttagtgtc gctgtcgaaa ttcatgtccc acacctgcga ggcgataagc gagcggggca
     3001 gcacttcgcc ctgatggcgg aggaaaaact ccagcagagt aaactcttta ctggtcagag
     3061 tgatgcgcgt gccgctgcgg gtgactttgc ggctgacgag atcgaccatc agatcggcaa
     3121 cctgaaactg actttcgata atcatcgccg ccccgcgccg caggagagtg cgcacccgcg
     3181 ccagcagttc agcaaaagcg aacggcttca ccagatagtc atccgccccc aactccagcc
     3241 ccttgacgcg atgttcaatg gtgccaagcg cggtaagcaa cagaatcggc atccctttat
     3301 tggcggaacg caacatgcgc acgatatccc agccgttcac gtccggcagc ataatatcga
     3361 ggattatcag atcataatca ccggtcatcg ccagatggta gccattcaac ccgttgtcgg
     3421 ccaaatcgac cacaaaaccg gcttcggtta accctttggt caagtattct ccggttttct
     3481 tttcatcttc gacaatcaac agtttcatat ttcctccgca tgttgcccgg gcaattctag
     3541 agtagcggga tcagatggca atcgcttatt ggcaaaatga caattttgtc atttttctgt
     3601 caccggaaaa tcagagcctg gcgagtaaag ttggcggcat aaaatcacca gaaattatga
     3661 gcctatgtct ccttgtaaac ttctgccatt ttgtgtggcc cttgcgctaa ccggttgttc
     3721 actggcaccg gattatcagc gtccggcaat gcccgtgccg cagcagttct cactcagcca
     3781 gaacggcctg gttaacgcag cagataacta tcagaacgcg ggctggcgca ccttttttgt
     3841 tgataatcag gtgaagacgc tgattagcga ggcgctggtg aataatcggg atttgcgcat
     3901 ggcgacgctg aaagtgcagg aggcacgggc gcaatatcgt ctgaccgatg ccgaccgcta
     3961 cccacagctc aatggcgagg gcagcggcag ctggagcggt aatcttaaag ggaataccgc
     4021 cacgacacgg gagttttcga ccggccttaa cgccagcttt gacctcgatt ttttcggtcg
     4081 cttaaagaac atgagcgaag ccgagcgaca aaattattta gccactgagg aagctcagcg
     4141 cgcggtgcat attctgctgg tttctaatgt cgcgcaaagc tatttcaatc agcaactggc
     4201 gtatgcgcaa ttgcaaatag ccgaagaaac gctgcgtaat tatcagcagt catatgcgtt
     4261 tgtcgaaaaa caactgttga caggtagcag taatgttctg gcgctggaac aggctcgcgg
     4321 ggtgatagaa agtacccgca gcgacatcgc taaacgtcag ggggaactgg cgcaggcgaa
     4381 taatgcattg caactgttgc tgggaagcta cggcaagctg ccgcaagcgc agacagtaaa
     4441 cagcgacagc ctgcaaagcg ttaaattacc ggcgggattg tcgtcgcaaa tcttattgca
     4501 gcgcccggat attatggaag ctgaacacgc gttaatggcg gctaatgcca atattggcgc
     4561 tgcacgcgcg gcattttttc cgtctataag cctgaccagc ggaatatcga ccgccagtag
     4621 cgatctatcg tcattattta acgccagcag cgggatgtgg aattttatac ccaaaattga
     4681 gatccccatt tttaatgccg gacgcaacca ggccaatctg gatatcgccg aaattcgcca
     4741 gcagcagtcg gtggtgaatt atgaacagaa aatccagaac gcctttaaag aagtggcaga
     4801 tgcgctggca ttacgtcaaa gcctgaacga tcaaattagc gcccagcagc gttatctggc
     4861 atcgctgcaa attactttgc aacgggcgcg ggcattatat cagcacggcg cagtaagtta
     4921 tctggaagtg ctggatgccg agcgttcttt atttgcaacc cgacaaactt tacttgatct
     4981 gaattatgcc cgtcaggtta acgaaatttc tttatatacc gcacttggtg gcggttggca
     5041 gcaataactt ttaactccag gagagaataa atgaaaaaag cactgcaagt cgcaatgttc
     5101 agtctgttta ccgttattgg ctttaatgcc caggctaacg aacatcatca tgaaaccatg
     5161 agcgaagcac aaccacaggt tattagtgcc actggcgagg taaagggtat tgatctggaa
     5221 agcaaaaaaa tcaccatcca tcacgatccg attgctgctg tgaactggcc ggagatgacc
     5281 atgcgcttta ccatcacccc gcagacgaaa atgagtgaaa ttaaaaccgg cgacaaagtg
     5341 gcgtttaatt ttgtccagca gggcaacctt tctttattac aggatattaa agtcagccag
     5401 taacccaggt ttaatgagat gaaaaaaatc gcgcttatta tcggcagcat gatcgcgggc
     5461 ggtattattt ctgcggcagg ctttacctgg gttgcaaagg cggaaccgcc tgcagaaaaa
     5521 acgtcgaccg cagaacgtaa aatcttattc tggtacgacc caatgtatcc caatacgcgg
     5581 ttcgataaac caggtaaatc gccg
//
LOCUS       DAMZET010000225         5580 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.225, whole genome shotgun sequence.
ACCESSION   DAMZET010000225 DAMZET010000000
VERSION     DAMZET010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.225"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            218..982
                     /gene="puuD"
                     /locus_tag="POB34_004367"
     CDS             218..982
                     /gene="puuD"
                     /locus_tag="POB34_004367"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HDI5921205.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            1009..1566
                     /gene="puuR"
                     /locus_tag="POB34_004368"
     CDS             1009..1566
                     /gene="puuR"
                     /locus_tag="POB34_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HDI5921206.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            1716..3203
                     /gene="puuC"
                     /locus_tag="POB34_004369"
     CDS             1716..3203
                     /gene="puuC"
                     /locus_tag="POB34_004369"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HDI5921207.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQQAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            3205..4485
                     /gene="puuB"
                     /locus_tag="POB34_004370"
     CDS             3205..4485
                     /gene="puuB"
                     /locus_tag="POB34_004370"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HDI5921208.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTATGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKMLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            4523..>5580
                     /gene="puuE"
                     /locus_tag="POB34_004371"
     CDS             4523..>5580
                     /gene="puuE"
                     /locus_tag="POB34_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HDI5921209.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLTNTL
                     IDAKESVPAIAAVRGLGS"
BASE COUNT         1313 a         1513 c         1572 g         1182 t
ORIGIN      
        1 ttccatgatt cttcgccttt ggtttgtttt ccgctcgtta tcaaagcgta aaatataatg
       61 accaccattc gaatctgtat gcaaactaaa tgtttgtcaa atgttaaatt gagtttgcaa
      121 aaatgaaaac ccactgctag attgaaaaaa tattgaacat aaaggtcatt taaaccgcag
      181 taatggcgat aatttagtcc actttgtgag attgagcatg gaaaatataa tgaacaatcc
      241 ggttatcggt gtcgtaatgt gcaggaacag gcttaagggt catgcgaccc agactctgca
      301 agaaaagtac ctgaatgcca tcatccacgc aggcggcttg ccaattgcgc taccacatgc
      361 gctggcggaa ccgtcattac ttgaacaact tttgccgaaa ctcgatggca tttatcttcc
      421 tggtagtccc agcaatgtgc agccgcacct atatggtgaa aacggcgatg agcctgacgc
      481 cgatcccggg cgtgatcttc tgagcatggc gttaattaat gccgcactcg aaaggcgcat
      541 ccccattttc gccatctgcc ggggtttaca ggaactggtg gtggcaaccg gtggttcgtt
      601 gcatcgcaag ctgtgcgaac agcctgaatt gctggaacat cgggaagatc ccgaactgcc
      661 ggtggaacag caatatgcac cgtcgcatga agttcaggtt gaagaggggg gattactgtc
      721 tgcgttgtta cctgaatgta gcaacttttg ggtaaactct ctgcatgggc aaggggcgaa
      781 ggtcgttagc ccacggttgc gtgttgaagc tcgctcgccg gatggcttag ttgaggcggt
      841 tagcgtcatc aatcatcctt ttgcgctggg cgtacagtgg cacccggaat ggaacagtag
      901 cgagtacgcg ctttcgcgta tattgttcga gggctttatc accgcttgtc agcaccatat
      961 cgctgaaaaa cagcgactct gaccactaca gtttaaggaa atgcaaatat gagtgatgag
     1021 ggactggcgc caggaaaacg cttgtcggaa atccgccagc agcaggggct ttcacaacgt
     1081 cgtgccgccg aactctccgg gctgactcac agtgctatca gtacgataga acaagataaa
     1141 gtcagccctg ccatcagtac gctgcaaaag ctgctgaagg tgtatggtct gtcactctcg
     1201 gaattctttt ccgagccgga aaaacctgat gagccgcagg tcgtcattaa tcaggacgac
     1261 ttaattgaga tgggcagtca gggtgtgtca atgaagctgg ttcataacgg taacccgaat
     1321 cgcacgctgg cgatgatctt tgaaacgtac cagccgggca caaccactgg ggaaagaatt
     1381 aagcatcagg gtgaggaaat aggcacagta ctggaaggtg aaattgttct gacgattaat
     1441 ggtcaggatt accacctcgt cgcggggcaa agttatgcca ttaataccgg catcccgcac
     1501 agtttcagta atacgtcggc aggtatttgc cgaattatca gcgcccatac gcccaccacg
     1561 ttttaatctt tttgttctgt aagccgggta agcgcaatgc gcccggcaat ccataaagaa
     1621 tgtcgccaga aataaacggg catacggcct ggggatctct gcgccccgac gttcacaaac
     1681 tgcatatatc tgatagacgt gaaacaggag tcataatgaa ttttcatcat ctggcttact
     1741 ggcaggataa agcgttaagt ctcgccattg aaacccgctt atttattaac ggtgaatata
     1801 ctgctgcggc ggaaaatgaa acctttgaaa ccgttgatcc ggtcacccag gcaccgctgg
     1861 ccaaaattgc ccgcggcaag agcgtcgata tcgaccgtgc ggtgagcgca gcacgcggcg
     1921 tatttgaacg cggcgactgg tcactctctt ctccggctaa acgtaaagct gtactgaata
     1981 aactcgccga tttaatggaa gcccacgccg aagagctggc actgctggaa actctcgaca
     2041 ccggcaaacc gattcgtcac agtctgcgtg atgatattcc cggcgcggcg cgcgccattc
     2101 gctggtacgc cgaagcgatc gacaaagtgt atggcgaagt ggcgaccacc agtagccatg
     2161 agctggcgat gatcgtgcgt gaaccggtcg gcgtaattgc cgccatcgtg ccgtggaact
     2221 tcccgctgct gctgacttgc tggaaactcg gcccggcgct ggcggcggga aacagcgtga
     2281 ttctaaaacc gtctgaaaaa tcaccgctca gtgcgattcg tctcgccggg ctggcgaaag
     2341 aagcaggtct gccggatggc gtactgaacg tagtaacggg ttttggtcat gaagcggggc
     2401 aggcgctttc gcgtcataac gatatcgatg ccatcgcctt taccggttca acccgtaccg
     2461 ggaaacagct gctgaaagat gcgggcgaca gcaacatgaa acgcgtctgg ctggaagcgg
     2521 gcggcaaaag cgccaacatc gttttcgctg actgcccgga tttgcaacag gcggcaagcg
     2581 ccaccgcagc aggcatcttc tacaaccagg gtcaggtgtg catcgccgga acgcgcctgt
     2641 tgctggaaga gagcatcgcc gatgaattct tagccctgtt aaaacagcag gcgcaaaact
     2701 ggcagccggg ccatccactt gatcccgcaa ccaccatggg caccttaatc gactgcgccc
     2761 acgccgactc ggtccatagc tttattcggg aaggcgaaag caaagggcaa ctgttgttgg
     2821 atggccgtaa cgccgggctg gctgccgcca tcggcccgac catctttgtg gatgtagatc
     2881 cgaatgcgtc cttaagccgc gaagagattt tcggtccggt gctggtggtc acgcgtttca
     2941 catcagaaga acaggcgcta cagcttgcca acgacagcca gtacggcctt ggcgcggcgg
     3001 tatggacgcg cgacctctcc cgcgcgcacc gcatgagccg acgcctgaaa gccggttccg
     3061 tcttcgtcaa taactacaac gacggcgata tgaccgtgcc gtttggcggc tataagcaga
     3121 gcggcaacgg tcgcgacaaa tccctgcatg cccttgaaaa attcaccgaa ctgaaaacca
     3181 tctggataag cctggaggcc tgaaatgacc gaacatacca gcagttacta cgccgccagt
     3241 gcgaataaat acgcgccatt cgacacgctg aatgagtcga tttcctgcga cgtttgcgtg
     3301 gtcggcggcg gctataccgg gctttcctcc gcactgcatc tggcagaagc gggctttgac
     3361 gtagtggttc tcgaagcctc acgcatcggc tttggcgcca gcggacgcaa cggcgggcag
     3421 cttgtgaact cctacagccg cgacatcgat gtgatcgaaa aaagctacgg catggacacc
     3481 gcccgtatgc tcggcagcat gatgttcgaa ggtggtgaga tcatccgcga acgtatcaaa
     3541 cgttatcaga ttgactgcga ctaccgcccc ggcggtctgt ttgtggcgat gaatgataaa
     3601 caactcgcca cactcgaaga gcagaaagag aactgggaac gctacggcaa taaacaactg
     3661 gaactgctgg acgccaacgc cattcgccgt gaagtggcca gcgaccgcta caccggcgcg
     3721 ctgttggatc acagcggcgg acatattcac ccgttgaacc tcgccattgg cgaagcggac
     3781 gctatccgcc tgaacggcgg gcgcgtgtat gagctttccg cagtgacgca gattcagcac
     3841 accacgcccg ccgtagtgcg tacagccaca ggtcaggtga cggcgaagta tgtgattgtc
     3901 gccgggaatg cgtacctggg cgataaagtg gagccggagc tggcgaaacg cagcatgccg
     3961 tgcggcacgc aggtgatcac caccgagcgg ctgtcggaag atttagcccg ttcccttatc
     4021 ccgaaaaact actgcgtcga agactgtaac tatctgcttg attactaccg tcttaccgcc
     4081 gataaccgcc tgctgtacgg cggcggcgta gtgtatggcg ctcgcgaccc ggacgatgtt
     4141 gagcgccttg tggtgccgaa aatgctgaaa accttcccgc agctgaaggg cgtgaaaatt
     4201 gattaccgct ggacgggcaa cttcctgctg accctgtcgc gtatgccgca gtttggtcgc
     4261 ctcgatacca acatctatta catgcagggc tacagcggcc acggcgtcac ctgtacccac
     4321 ctcgccgggc gtctgattgc cgaactgctg cgcggcgacg ccgaacgttt cgatgccttc
     4381 gccaatctgc cgcattaccc gttccccggc gggcgcacgc tgcgcgtacc gtttaccgcg
     4441 atgggcgcgg cgtattacag cctgcgcgat cgtctgggcg tttaatttcc gattaacagt
     4501 gaagagtcaa aaggtgtgaa acatgagcaa caatgaattc catcagcgtc gtctttctgc
     4561 caccccgcgc ggggttggcg tgatgtgtaa cttcttcgcc cagtcggctg aaaacgccac
     4621 gctgaaggat gttgagggca acgagtacat cgatttcgcc gcaggcattg cggtgctgaa
     4681 taccggacat cgccaccctg atctggtcgc ggcggtggag cagcagctgc aacagtttac
     4741 ccacaccgcg tatcagattg tgccatatga aagctacgtc accctggcgg agaaaatcaa
     4801 cgcccttgcc ccggtgagcg ggcaggccaa aaccgcgttc ttcaccaccg gtgcggaagc
     4861 ggtggaaaac gcggtgaaaa tcgcccgcgc ccataccgga cgccctggcg tgattgcgtt
     4921 tagcggcggc ttccacggtc gtacgtatat gactatggcg ttaactggaa aggtcgcgcc
     4981 gtacaaaatc ggcttcggcc cgttccctgg ttcggtgtat cacgtacctt atccgtcaga
     5041 tttacacggc atttcaacac aggactccct cgacgccatc gaacgcttgt ttaaatcaga
     5101 catcgaagcg aagcaggtcg cggccattat tttcgaaccg gtgcagggcg agggcggttt
     5161 caacgttgcg ccaaaagagc tggttgccgc cattcgccgc ctgtgcgacg agcacggtat
     5221 tgtgatgatt gctgatgaag tgcaaagcgg ctttgcgcgt accggtaagc tgtttgccat
     5281 ggatcattac gccgataagc cggatttaat gacgatggcg aaaagcctcg cgggcgggat
     5341 gccgctttcg ggcgtggtcg gtaacgcgaa tattatggac gcacccgcgc cgggcgggct
     5401 tggcggcacc tacgccggta acccgctggc ggtggctgcc gcgcacgcgg tgctcaacat
     5461 tatcgacaaa gaatcactct gcgaacgcgc gaatcaactg ggccagcgtc tgacaaacac
     5521 gctgattgat gccaaagaaa gcgttccggc catcgcggcg gtacgcggtc tggggtctat
//
LOCUS       DAMZET010000226         5579 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.226, whole genome shotgun sequence.
ACCESSION   DAMZET010000226 DAMZET010000000
VERSION     DAMZET010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.226"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1452)
                     /gene="fadK"
                     /locus_tag="POB34_004372"
     CDS             complement(<1..1452)
                     /gene="fadK"
                     /locus_tag="POB34_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HDI5921210.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKTGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTSLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKA"
     gene            complement(1509..1802)
                     /locus_tag="POB34_004373"
     CDS             complement(1509..1802)
                     /locus_tag="POB34_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDI5921211.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(1799..3088)
                     /locus_tag="POB34_004374"
     CDS             complement(1799..3088)
                     /locus_tag="POB34_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDI5921212.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNNDSVSLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(3144..4082)
                     /locus_tag="POB34_004375"
     CDS             complement(3144..4082)
                     /locus_tag="POB34_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HDI5921213.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(4102..4866)
                     /locus_tag="POB34_004376"
     CDS             complement(4102..4866)
                     /locus_tag="POB34_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HDI5921214.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLTTDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQPQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            5182..>5579
                     /locus_tag="POB34_004377"
     CDS             5182..>5579
                     /locus_tag="POB34_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDI5921215.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFN"
BASE COUNT         1327 a         1474 c         1410 g         1368 t
ORIGIN      
        1 cgctttcagc acgacataag cgcatgatcg ttcacctaaa cgttcatcgg gcattgcaac
       61 cacacaggca tcgtgaattt taggatgctg caataaaata tcttccactt cacggctgct
      121 aatgttttcg ccgccgcgga caataatatc ttttttgcgt ccggtaattt ttatatagcc
      181 agcctcatcc atgcggcaga gatcgccgct gtaataccag ccttcttcgt ccagcgcacg
      241 ggcggttaat tcaggttcat caaaataccc cataaacaca ttggggccac gcgaggcttc
      301 ttcaccttcg caacctggcg gtaaggtctt gcgtgcgtca tcgaccactt taatctctac
      361 acctgcggca gcgtaaccat cggtgtgcat aaagcgcgac aaaggatcat cgagattcac
      421 caccgcatgc ggcgaacttt ctgtggaacc ataaacactt aataatttaa tgccgcgctg
      481 ctggcattca cgtgccactt ttttggggat tgtggtaccg ccgcaaagaa agaaacgcag
      541 cgctgaaagg tccgcgggtt gtttctctag tacattcaaa agatcataga caaacggcgt
      601 tgcgcctagc atacaggtgc aacgctgctg ctcaagcagc gcgagacacg catcaggagt
      661 gaaaatatct aacaacacgc tgcgagcgcc aattaagaat ggtgccgtta cgccatgcag
      721 aaagcccgtt gcgtgaccaa gtggcgcagg catcataaag acatcctgcc aggtcagatt
      781 cagtcgcgcg caataagccc gctcactggc gagaatattg ttatgcgtta gcatcacgcc
      841 ctttggcaga ccctcggttc cggaggtaaa cagcaccgca gctaattcat cgccgtgggt
      901 cgttatcgcc gtggtcagtg aggtattgtc ggcgataatc tgacttaatg agagggaaga
      961 ggtggcggga gccagtttgt ccacaccgac aatttgttgt agttgtggaa gctgattttg
     1021 cagcggcagg attaaatcta ccggacgcgt ttgtttaaac aacgtcggtg caaagaacat
     1081 ttttgcctga cacttattga gcacccacac cagttctgct tcccgccagg aaggcaacag
     1141 cggcacggaa accgcaccgg ttttcaggca ggcaagatag ataacggtaa attcacacca
     1201 gcctggcagt tgaaatgcga tgcgatcgcc tgattcaata cccttcgcta acatccagtt
     1261 tgccagacag ctcgcggcgt gatcgagcgc gctataggtg tacgatgctc catgattatc
     1321 gaccacggca attttgtctg gcatcgcacg agcggtctgc tgccagtaat cggccagcga
     1381 agcatcgccc catagccctt gctgacgata cgccgcacga cgttgttcgt tgaacgttaa
     1441 tgtcactttc atgttggact ctcgaaacag aacatcaggc ccgagatgcg ggcctgtggg
     1501 atgcatgatt agccgtagcg aaaatcaata ccgaaggtgc ctgcgggata ttgccactgt
     1561 tcgagaatag tgttaccgca cagcacccga caggtgccgc actccagaca accggcagaa
     1621 tcaaaatgaa tgttgccggc gtcatcctgc ttataaagtc cggcagggca ggctttcatt
     1681 aatttacgga attcattgat atcgggattt gccgccagaa tgatatgcgg gtggccctca
     1741 tcaacatgga atttattgac gcctaatttg atgtcgacgt taaccgtagc gttctggctc
     1801 atagcgcggt tgctccctta atgccatctt tcagcaagtt gatcagccca attttcttcg
     1861 catgtcccat gatcattttg cgtaccggct ggtttggttt gccgtcaatg gtgaacatct
     1921 cgttcatgat gtcggcgacc attcgtgggt attggctaaa caggcgcggg ttttccatca
     1981 gcgccgggat cttgcggaaa tgctgcatat cgcgcatgac gcagctttgt tccagctcac
     2041 gtttgtattg cgccagactg ctggcggaga aatcctcgcg ttctttggcg gcgatcaccg
     2101 ttgtggcggc agcctgagcc gatgcaatgg ctaaatccat gccacgaact gtaaaaccca
     2161 aattcaggca gaagcctgcg gcgtcaccaa cgatcatcac gccatcgtta accagctgcg
     2221 gcaccattgc cagaccgcct tctggcacca tatgcgcgga atattcaagc agtttgccgc
     2281 cgctaattag tgggcgaatg gcggggtgtt gtttaaaatc ttccagcatt tgcggcacgc
     2341 ttttttgcgc atgggcgata tcacccaatc cacaaaccag ccccaacgat acggaatcgt
     2401 tgttggtata gaggaatccg ccacccatca ggccgtcaga aggggaaccg gcaaacagcc
     2461 aggcggcacc ttcctcgccc gtaatattaa agcgatcgtt gatctgttct ggcgtgaggc
     2521 caataacctc tttaacacca acagcgtaat gatgcggatc ggaagcggga accattccca
     2581 gcgagcggcc aagcatcgag ttaacgccat cagccagaat caccacattc gcttcgagaa
     2641 tatcatcccc agcctgcacg ccagttacct tgtttccttc acgaaccagc gcatcgacgc
     2701 gaacgcctgg gataaactgc gcgcccgcct gctcggcttg ttccatcaac cacgggtcca
     2761 gacgattacg caatacggta taagatgcgt gttgcggaac atctggctgc tcgcggtgaa
     2821 aatcgagggt aacggcgctt tcttcggtta agaaggagat tttctcgcgt gtgaccttgc
     2881 gttctaccgg cgctgatgct gcaaagcctg gaatgattgc ttcaagtgtg tgggcataaa
     2941 gacgcccgcc ggtcatgttt ttacatccgg cactgtcgcc gcgttctatc accaggacat
     3001 ccagcccggc tcgtgccatg accagtgcgg caacgctacc agcaacgccc gcaccgacca
     3061 caatggcatc aaatttgtca tccgacatgc gttatgctcc cttcccgctg cgtttacagc
     3121 gggagatgtg gttgaaaggg gaattagcgg ctcaactggc taatcagggc agggacgact
     3181 ttgtagatat cgcccaccag accgtagtcg gcatagttga agattggcgc atttttatct
     3241 ttattgatgg cgacaatcac ttttgcgccg ttgccgccaa ccatatgttg gatctgcccg
     3301 gagatcccca gcgtcaggta gagatcagat ttcagcaaca cgccggagac accgatataa
     3361 cgttcacgct ccatccagtt ctcgccttcg gcaattggac gtgaacagcc gacttcagca
     3421 ttcagcaccg ccgccagttc gtggaccatt ttcaggtcat cctgcgccgc aagaccacga
     3481 ccgacgccaa ccacacgttt tgctttgctc aggtccacgc tgcttgcggc tttcgcacgg
     3541 cgttcgcgac agagaatttc atgacgcgga gcaacataag gtaccgtttc tgtcgggcac
     3601 tgatgagagg tatcactggt gcacggttcc tgaacaccgg gtgcaagggt aatgatcgcc
     3661 agcgggctgt tgatcttttc ctgagcgaac gctaacccgc catacatccg gtgttcggcg
     3721 caaatgtgac catcgacaat atccaccgcc gtggcatcgt tcaccagcgc cgcattcagt
     3781 tgcacactta accgtgctgc cagagcttta ccacgtttcg tcgcggccaa cagcaacata
     3841 gcggggtgtt tatctttcag cagggcagca atgctttcgg cgtaattttc agtgcgttgc
     3901 agcgcgtcgt tttgctcaag aacataaata cattttggac cataaggcat aactgcctgc
     3961 gcctggtcgg tattttgtac aatggcatac acctgttggc cccattgctg agcgccgcca
     4021 aacagttcag cataacgttc aggattatcg ctaaagaccc agacgctgtt taattgactc
     4081 atcgtttctt ctccgtaggc ttcagttcag ggctttcttc agatgttccg ccagctcggc
     4141 aatggcctcc ggcgaatcgt tatcgataat gatgtgctta cgttctgttt gcggcggtac
     4201 gcgaatgccg accagttcag caagtggcgc gctctggctc cagtcaatat cacttgcctg
     4261 ccactgattt accggttttt tacccgcgcc gagaatggct ttcatcgaag gaatgcgtgg
     4321 cacgttaata tcggaggtga cgcagagcac ggctggaaca gagagttcaa taacttcaac
     4381 atcatcttca agcgtgcgtt caatcaccag tgtattgccc tgaggctgaa tagcactcac
     4441 tgcattaatc accggaagtt gcagaatttc tccgaccagc aagccaacct gctgggcata
     4501 aaggtcgccg gaaccttcac caaagatcag taaatcgaag ccgatctttt caattgctgc
     4561 cgccagcgct tttgcggtat cgagaggcag tgcatgttca agttgcgcat cctgcaccaa
     4621 atacaggctg tgcggcccgc gggatagcac gtctttgcgc actttcgagt tctgcaacaa
     4681 tgagccgcca acggtcagcg cggctatctc atcgtcatct gttgtgagct ggcttgcagc
     4741 ttcaatggca ttgagatcga actggctgat tttggcgtcg gcattgtcga aattcagggt
     4801 gtattctgga gtgacaacaa tgtcctgttc ttcaggcacc agcttaaagc aggttattat
     4861 tttcatgatt tctcctgtga gtcgcatacg gaaccatctc cgatggaagg taaaaatggc
     4921 ctgaaaatag tgtgcgatag cggtgcgggg ttttcgacgg agcaagctgt tttccttgcc
     4981 aacaatcttg caaaaaaaat gcgatcgcta aaacaaaagt gcaatttgcg gcaatatagt
     5041 tattccatga ccgtacaatc ttgcattcag tgtaaataat cttgtttagc tctcgctaaa
     5101 cgcggttgta taaccgcaat acacgccgta ttttatccgg catatattgt ttttgcttgc
     5161 tcatggaata gggaggttgt catgtatcaa cgctgttttg ataacgccag tgaaacgctg
     5221 tttgtcgccg gtaaaacgcc acggctttca cgttttgcat ttagcgatga tccaaaatgg
     5281 gagtccggac atcacgttca tgacaatgaa accgagttga tttacgtcaa gaaaggggtt
     5341 gcaaggttta ccatcgattc ttcgttatat gtcgcacatg cggatgacat tgtggtgata
     5401 gaacgcggca ggctgcatgc ggtggcctct gacgttaacg atccggcaac gacgtgtacc
     5461 tgtgcgctgt acggctttca gtttcagggg gctgaggaaa atcagctact gcaaccgcat
     5521 tcttgtccgg taattgccgc ggggcaggga aaagaagtca taaaaacctt atttaatga
//
LOCUS       DAMZET010000227         5549 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.227, whole genome shotgun sequence.
ACCESSION   DAMZET010000227 DAMZET010000000
VERSION     DAMZET010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.227"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..277
                     /locus_tag="POB34_004378"
     CDS             <1..277
                     /locus_tag="POB34_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HDI5921216.1"
                     /translation="AFDDWFARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRA
                     IEAAEKGDMTELHRLHEALRNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(281..472)
                     /gene="hemP"
                     /locus_tag="POB34_004379"
     CDS             complement(281..472)
                     /gene="hemP"
                     /locus_tag="POB34_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HDI5921217.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(604..1650)
                     /gene="aroH"
                     /locus_tag="POB34_004380"
     CDS             complement(604..1650)
                     /gene="aroH"
                     /locus_tag="POB34_004380"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HDI5921218.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYYEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(1807..2640)
                     /gene="ppsR"
                     /locus_tag="POB34_004381"
     CDS             complement(1807..2640)
                     /gene="ppsR"
                     /locus_tag="POB34_004381"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HDI5921219.1"
                     /translation="MENAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            2973..5351
                     /gene="ppsA"
                     /locus_tag="POB34_004382"
     CDS             2973..5351
                     /gene="ppsA"
                     /locus_tag="POB34_004382"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HDI5921220.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPGLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
BASE COUNT         1345 a         1346 c         1500 g         1358 t
ORIGIN      
        1 ggcatttgat gactggtttg cccgttatcg ggggcgtttg caacaagacg aggttagcga
       61 tagtgagcgt cagcagctga tgcaaagcgt taacccagct ctggtgttgc gcaactggtt
      121 ggcgcaacgg gcgattgaag cggcagaaaa gggtgatatg acggaattgc accgcctgca
      181 tgaggcgttg cgaaatcctt tcagcgacag agctgatgac tatgtcagtc gtccacctga
      241 ctggggtaaa cggctggaag tcagctgctc gagttaaagg ctacttggtc aacagcagct
      301 tgccagcctg agttttacgg agcagatatt cttgcccgtc atgatcgata atcagtttgc
      361 catccggacc taacagtgtc tggctggaaa ttcgccgaat aggctgcggg gaagcggtct
      421 tgtgattggc atccgtttca ggcgtgagtt ttctgctatc cgtataacgc ataaaaatta
      481 ataataattg atataacaat gattcttatt atcaatatta tggctgacca gagcaagcag
      541 taatgtgctg agaagacaac aagttgcgca gaaatgccca tcccatcagg aatgggcacg
      601 cattcagaag cgggtatcta ccgcagaggc gagtttttcg accaggcgtt cggtatcttc
      661 ccagcccaga cacgggtcgg taatggattg accgtaagtg agcggctgac cgccgacgat
      721 tttttgcgtt ccttcgcgca ggaaactttc cgccataatt ccagcaatcg ccgtagagcc
      781 attgcggatt tgctgacaaa tatcctcaca aacttctaac tggcgacggt gctgcttctg
      841 gcagttaccg tggctgaaat ccaccaccag atgttcaggt aaatcaaact cgtgcagcgt
      901 atcgcaggct gcggcgatat catcggcatg ataattcggt tttttgccgc cacgcataat
      961 aatgtggcca tacgggttgc cgctggtctg atagatggtc atctgaccat ttttgtctgg
     1021 cgagagaaac atatggctgg cgcgggctgc gcggatagca tccacggcaa tccgcgtgtt
     1081 gccatcggta ccatttttaa aacctaccgg acaggagagt gccgaagcca tttcacggtg
     1141 gatctgactt tcggtagtac gtgcgccaat cgctccccaa ctgattaaat cagcaataaa
     1201 ctgaccggtc accatatcga ggaactcagt cgcggtaggg acacccagct catttacctg
     1261 taaaagtaat ttgcgcgcca gctccagacc gtgatttacc cgatagctgc cgtttaaatc
     1321 tggatcggag attagccctt tccagccgac aacagttcgg ggtttttcat aataggtgcg
     1381 cattacgatt tcaagccgtg actggtactg gttgcgcagc gactgcagac gggtggcgta
     1441 ttccattgca gcggtgagat cgtggatcga gcaggggcca ataatgacca acagtcgctt
     1501 atcttcacca ttcagtattt tttcaattct gcggcgggag tcggtgacat gggtggcgac
     1561 gccaggcgtt acgggatacc gtagcgcgag ttcggcgggc gttaccaggc tctcaatacg
     1621 cgcagtacgg agttcgtcag ttctgttcat tacaagtctc agaaatttgt tgcttctacg
     1681 gcaggactac cgggaagtga tgcgcatcac gataaaccaa tcgcagctga tttcaagtgc
     1741 gaggtgtgtc acacctcgcc gggtggttag catgataaca aaaaaacaag ttaatgcact
     1801 agttctctag tacattcgac ggctaaggcc catgatatcg aggatcttgg tggcaatctc
     1861 ttctaccgaa taattggtac tgttaatcca cgggatctga tttttacggt acaacgcttc
     1921 cacttccgcg acttccatcc tgcactgacg aagcgaggca tagcgactgt tctcccgacg
     1981 ttcctcgcga atcgccgcca gtcgttccgg gtcgatagtc aggccgaaca atttatgctg
     2041 aagcggtttg agcgacgcgg gtagcaccag attatccata tcgtcggcaa taaaggggta
     2101 gtttgccgcg cggataccaa actgcattgc cagatacaga ctggtggggg ttttaccaca
     2161 gcgagaaaca ccgagcagga tcacctgagc ctggtccaga ttgcgcaacg aaatgccgtc
     2221 atcgtgggcg agggtgtaat caatcgccgc aatgcgcgca tcatatttat tgagattatt
     2281 agggttaagg ccatgggtac gatgagcaat cggcgttgga tccagtttca tctcttgttg
     2341 tagcggggca accagcgcct gaacgatatc ctggcaaaaa ccttcacttt gcaagatgat
     2401 ggcgcgaatc tccggcaaca cgatggagta gaagaccagc gggcgcacac ctgtctggtg
     2461 ataaattgcg tcaatctgat ccttcactgc ccgtgcacgg ctctcatttt cgacaaacgg
     2521 cagcgtaatg ctgctgatag tgacgggaaa ttgtgacatt actgcgtgtc ctaatacctc
     2581 cgcagttatt gccgtaccat cagaaatata aaaaacgtgg cgatcaacag cattttccat
     2641 tttgttcttc ccgtgatgca gacataattc tatgaaagca taaattaaaa acgcacagaa
     2701 gcgtaggacg taatgtctgg tttataaaat gaaccttcaa ttttattttt tatgaaaaca
     2761 gcatttcatt tttatggttt cgtttatacc gatggtttat gtggaaattg tcgaatagag
     2821 caggtttgcg caacgctggg atcagtctta aaaagtaaaa aaatatattt gcttgaacga
     2881 ttcaccgttt ttttcatccg gttaaatatg caaagataaa tgcgcagaaa tgtgtttctc
     2941 aaaccgttca tttatcacaa aaggattgtt cgatgtccaa caatggctcg tcaccgctgg
     3001 tgctttggta taaccaactc ggcatgaatg atgtagacag ggttgggggc aaaaatgcct
     3061 ccctgggtga aatgattact aatctttccg gaatgggcgt ttccgttccg aatggtttcg
     3121 ccacaaccgc cgacgcgttt aaccagtttc tggaccaaag cggcgtaaac cagcgcattt
     3181 atgaactgct ggataaaacg gatattgacg atgttaccca gcttgcgaaa gcgggcgcgc
     3241 aaatccgcca gtggattatc gacactccct tccagcctga gctggaaaac gccatccgcg
     3301 aagcctatgc acagctttcc gccgatgacg aaaacgcctc gtttgcggtg cgttcctccg
     3361 ctactgcaga agatatgccg gacgcttctt ttgccggtca gcaggaaact ttcctcaacg
     3421 ttcagggttt tgacgccgtt ctcgtggcag tgaaacatgt atttgcttct ctgtttaacg
     3481 atcgcgccat ctcttatcgt gtgcaccagg gttacgatca ccgtggcgtg gcgctctccg
     3541 ccggtgttca gcggatggtg cgctccgacc tcgcatcttc tggcgtgatg ttctccattg
     3601 ataccgaatc cggctttgac caggtggtgt ttatcacttc cgcatggggc cttggggaaa
     3661 tggtcgtaca gggtgcggtt aacccggatg agttttacgt gcataaaccg acactggcgg
     3721 cgaatcgccc ggctatcgtg cgccgcacca tggggtcgaa aaaaatccgc atggtttacg
     3781 cgccgaccca ggagcacggc aagcaggtta aaatcgaaga cgtaccgcag gaacagcgtg
     3841 acatcttctc gctgaccaac gaagaagtgc aggaactggc aaaacaggcc gtacaaattg
     3901 agaaacacta cggtcgccca atggatattg agtgggcgaa agatggccac accggcaaac
     3961 tgttcattgt gcaggcgcgt ccggaaaccg tgcgctcacg cggtcaggtc atggagcgtt
     4021 atacgctgca ttcacagggt aagattatcg ccgaaggccg tgctatcggt catcgcatcg
     4081 gcgcgggtcc ggtgaaagtc atccatgaca tcagcgaaat gaaccgcatc gaacctggcg
     4141 acgtgctggt tactgacatg accgacccgg actgggaacc gatcatgaag aaagcatcag
     4201 ccatcgtcac caaccgtggc ggtcgtacct gtcacgcggc gatcatcgct cgtgaactgg
     4261 gcattccggc ggtagtgggc tgtggtgatg caacagaacg gatgaaagac ggtgagaacg
     4321 tcactgtttc ttgtgccgaa ggtgataccg gttacgtcta tgcggagttg ctggaattta
     4381 gcgtgaaaag ctccagcgta gaaacgatgc cgggcctgcc gttgaaggtg atgatgaacg
     4441 tcggtaaccc ggaccgagct ttcgacttcg cctgtctgcc gaacgaaggc gtgggacttg
     4501 cgcgtctgga atttatcatc aaccgtatga ttggcgtcca cccacgcgca ctgcttgagt
     4561 ttgacgatca ggaaccgcag ttgcaaaacg aaatccgcga gatgatgaaa ggttttgatt
     4621 ctccgcgtga attttacgtt ggtcgtctga ctgaagggat cgcgacgctg ggtgccgcgt
     4681 tttatccgaa gcgcgtcatt gtccgtctct ctgattttaa atcgaacgaa tatgccaacc
     4741 tggtcggtgg tgagcgttac gagccagatg aagagaaccc gatgctcggc ttccgtggcg
     4801 cgggccgcta tgtttccgac agcttccgcg actgtttcgc gctggagtgt gaagcagtga
     4861 aacgtgtgcg caacgacatg gggctgacca acgttgagat catgatcccg ttcgtgcgta
     4921 ccgtagatca ggcgaaagcg gtggttgaag aactggcgcg tcaggggctg aaacgtggcg
     4981 agaacgggct gaaaatcatc atgatgtgtg aaatcccgtc caacgccttg ctggcagagc
     5041 agttcctcga atatttcgac ggcttctcaa ttggctcaaa cgatatgacg cagctggcgc
     5101 tcggtctgga ccgcgactcc ggcgtggtgt ctgaactgtt cgatgagcgc aacgatgcgg
     5161 tgaaagcact gctgtcgatg gcgattcgtg ccgcgaagaa acagggcaaa tatgtcggga
     5221 tttgcggtca gggtccgtcc gaccacgaag actttgccgc atggttgatg gaagagggga
     5281 tcgatagcct gtctctgaac ccggacaccg tggtgcaaac ctggttaagc ctggctgaac
     5341 tgaagaaata aaataaatcc ccggcggcgt tcagtcgccg gggttatgtg atccccgaag
     5401 atgatactta ttcaatctct tcacagacat cctgcgttaa tcgccgcata atatcttttc
     5461 ttaacaaaaa cttttgtatt ttacctgagg cagtgcgcgg tagtttttcg attaccacga
     5521 tatgttcagg atatttatat tttgcgacc
//
LOCUS       DAMZET010000228         5548 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.228, whole genome shotgun sequence.
ACCESSION   DAMZET010000228 DAMZET010000000
VERSION     DAMZET010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.228"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            155..262
                     /locus_tag="POB34_004383"
     CDS             155..262
                     /locus_tag="POB34_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDI5921221.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(410..1264)
                     /gene="kdsA"
                     /locus_tag="POB34_004384"
     CDS             complement(410..1264)
                     /gene="kdsA"
                     /locus_tag="POB34_004384"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HDI5921222.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(1300..2109)
                     /locus_tag="POB34_004385"
     CDS             complement(1300..2109)
                     /locus_tag="POB34_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HDI5921223.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(2113..2505)
                     /locus_tag="POB34_004386"
     CDS             complement(2113..2505)
                     /locus_tag="POB34_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HDI5921224.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTMLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(2502..3335)
                     /gene="prmC"
                     /locus_tag="POB34_004387"
     CDS             complement(2502..3335)
                     /gene="prmC"
                     /locus_tag="POB34_004387"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDI5921225.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGKGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALARERPDCEITAVDRMPDAVALAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(3335..4417)
                     /gene="prfA"
                     /locus_tag="POB34_004388"
     CDS             complement(3335..4417)
                     /gene="prfA"
                     /locus_tag="POB34_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDI5921226.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(4459..>5548)
                     /gene="hemA"
                     /locus_tag="POB34_004389"
     CDS             complement(4459..>5548)
                     /gene="hemA"
                     /locus_tag="POB34_004389"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDI5921227.1"
                     /translation="LSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHL
                     MRVASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDI
                     GASAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTR
                     ERAQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPM
                     LLVDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEF
                     MAWLRAQSASETIREYRGQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIH
                     APTKSLQQAARDGDNERLNILRDSLGLE"
BASE COUNT         1350 a         1504 c         1360 g         1334 t
ORIGIN      
        1 tgcgggggct ttatccctgg tggcattggt tgctggaaag agaaaacccc cgcacgttga
       61 gaggtaaaac ctgacaacag aacgggggct aatcttgaac catcaacaat tcaagaatag
      121 ccgcaaaatg ttgtcattac aacaaggcgg ctatatgacg ctcgcgcagt ttgccatgat
      181 tttctggcac gacctggcag caccgatcct ggcgggaatt attaccgcag cgattgtcgg
      241 ctggtggcgt aaccggaagt aaggcgaaaa cgggcgtcag gctgccgtga tggcaatatg
      301 cgcccgaaac cggccgcagg ggtaacagcc tgcggccttt tttgtatccg gcacccataa
      361 aaaaagtctt aacgcagaac gctaagactt tatttttcaa acaaaaagat tacttgctgg
      421 tatccagttc ttcgaaacct ttcaccagat catcaatcgc tttcatctgc ttgaggaacg
      481 gttccagttt agccagcggc agcgcggatg gaccatcaca tttcgcatgt tccggatccg
      541 gatgcgcttc aataaacagc cccgccagac ctaccgccat accggctcgt gccagctcag
      601 ccacctgagc acgacgaccg ccggaagctg cgccaaaagg atcgcggcat tgcagtgcgt
      661 gggtcacgtc gaaaatcacc ggcgagttac cagacacttt cttcataatg ctgaagccca
      721 gcatatcgac aaccaggttg tcatagccga agttagcacc gcgatcgcaa agaatcactt
      781 tttcgttgcc gccttctttg aatttatcaa cgatattacc catctgtccc gggctgacaa
      841 actgtggttt cttgacgtta attaccgcac cggttttcgc catggcttca accaggtcag
      901 tctggcgagc aagaaacgcc ggcaactgaa tcacatccac gacatcagca acgggctgtg
      961 cctgacttgg ttcgtgaacg tcggtgataa ttttcacgcc aaaagtctgc ttcaattcct
     1021 ggaagatttt catcccttct tccaggcccg gtccacgata agagtggatg gaggagcggt
     1081 tggctttgtc aaaagaggct ttgaacacgt aagggatacc cagtttctgg gtcacggtta
     1141 cgtagtgctc gcaaatgcgc atcgccagat cgcgagattc cagcacgttc ataccaccaa
     1201 acagtacgaa cggcaggtca tttgctacgt tgatgtcgcc aatgctaacc actttttgtt
     1261 tcataggatc gccttaatct tgagtaaaat gtcgattaat taatgcagca caatatgttt
     1321 atgcgcgatg ttatttattt gcgcacggat catttcgctg atcgggtctt ccggacactg
     1381 ttcaacgaaa taacttaaat cgttcaacgc aacgtgttcg caatccagtt gcgcataaat
     1441 caacccgcga tcgcgaattt catagggatc ttcagggttg aattgtaata aagcttcgct
     1501 ggtgcgtaac gccagctcca tctgattttc ttccatcaac gaggctttga gtgtatccag
     1561 caatttgcgg attacctcaa tgttatcagc ttcatcaagg tcttcataaa acagttccgc
     1621 cgacgggctg atatttccct ttaaccatac gtccagcata tgttcgctta acgattcacc
     1681 gttaaaagga ttaatcagcc aaatttcgcc atccggacat tcaatgcgca atatcagctg
     1741 cgtagggaaa atcaccggca gcagcggcaa atcgagacga ttcgcgaccc ataataaaac
     1801 cgcacctaat gataccgcac tgccctgtcg attctttaac acctggtcca gccataatgc
     1861 atcggaaaga cgataaacgc cgcgtgaggc tttaaatccc cagtcgccgt aaaacagcgc
     1921 gatcagtttt tccagctgct cttctaaagg cagaagctgg ctgatttctt ccttcgccag
     1981 actaacgaga cgctccagtt cgtcgtaaac atcttgcgag ggaaaatcgc ggcggattgc
     2041 ttcgcaagcc aggatcatgc cttcgcacaa tggcgcttta ttaaattcga aatcagctaa
     2101 cgatctcatg acttacccca gtaacggtac ttttgtggtg gcgagtttaa tgatgatgta
     2161 cagcaccacc aacgccagcg ggaaggcaat aatgcgcgcc tgctgactgt gcatacgacg
     2221 ataatcgagt gcaataaaac ccaaaacgat ataaatgata actccaaaca gcttttcagt
     2281 cagccactgt gcctgcccag aaaatggcag gatgtgcgct ttagccatca acgcaatgcc
     2341 gcttaacaat aacatcgtat cgacaaccgg cggcacaatg cgcgtccagc gagcaaatgc
     2401 ctgtggatgc ttctgataac gtagccagaa acgtaaggtt aatagcccaa cagaaagcgc
     2461 gatactaata agatgaacac taagcagtgt agaaaaactt gtcattgata atagcggccg
     2521 agcgttacgc gctcgttatc accatagtca cggcaggttt cgacgtcatg ataccccgcg
     2581 aggataaatg cctgccgcac cgcttcgccc tgctgccagc catgttccag aagcagaaag
     2641 ccgccggata ccagcgcgtt acgcgactgt tcgatgatat gcacgatgtc tgccattcca
     2701 ctgtctgccg caaccagcgc agtgagcggc tcaaagcgga catcgccttg ttgaagatga
     2761 gggtcctgct cgtcaatata cggcggattg ctgacaatca tcgcaaactg ctgcccggct
     2821 agcgcgctaa accagtcgct ttgcagaatg tggatatttt taatcgccag atgctgggca
     2881 ttacgttgtg ccagggcgac agcatcgggc atacgatcta cagcggtaat ttcgcagtcc
     2941 gggcgctcgc gagccagcgc aagcgcaatc gccccggtac ccgtcccgag atcgagaata
     3001 cggcaaggtt gttcaggcaa ccgcgccagt gcctgctcca ccagacactc cgtatccggg
     3061 cgcggaatta aggtcgctgg cgaaacaaat aacggcaacg accagaattc tcgcaccccg
     3121 gttaaatgag caatgggttc accatcgcga cgacgtgtca gtagcgcatc aagttgctga
     3181 cattgttcgt cagtcagctg cgtttcacca aaggcgagga taaaagtacg ccctttgccg
     3241 gtaacatgtt ccagcaggat ttcagcatca cgccgcgggc tttcgctcgc ctgaagttgg
     3301 cttattgctt cacgtaacca gtgttgatat tccattattc ctgctcggac aacgccgcca
     3361 gttggtcggc ctgatgttcc tggataatcg gttcaatcag catatccagc ttaccttcca
     3421 tcacttcatc caggcggtag agcgtcaggt tgatgcggtg atcggtaacg cgcccctgcg
     3481 ggaagttgta agtacggtta cggtcgctgc gatcgccact ccccagcagg ttacgacggg
     3541 tagacgcttc ggcctgttgg cgttttgcca tttcagcagc gtggatgcga gcaccgagaa
     3601 cagaaagtgc tttagctttg tttttatgtt gtgaacgttc gtcctgacat tcaacaacaa
     3661 tcccggtcgg caagtgagta atacgaattg ccgaatcggt ggtgttaacg tgctgaccac
     3721 ccgcccctga cgagcggaaa gtatcaatgc gtaaatctgc tgggttgatg tccggcagtt
     3781 ctgcgtcagg cagttctggc attaccgcaa cggtacaagc agaagtatga atacgaccct
     3841 gcgattccgt agcaggaaca cgttgcacgc gatgaccgcc ggattcaaat ttcagacgac
     3901 catacacacc atcaccgcta attttggcga tgatctcttt ataaccacca tgttcaccct
     3961 cgctggcgct catgatttct acccgccagc ggcgggcttc ggcataacgg ctgtacatac
     4021 ggaacagatc gcctgcgaac agcgccgctt cgtcgccacc ggttccggca cggacttcga
     4081 ggaaggcgtt acgttcgtca tcaggatctt ttggtagtaa cagaacctgt aattgctgtt
     4141 ccagttgctc gcttttttct ttagcttcgc gcagttcatc ctgcgccatc tcacgcattt
     4201 caggatcgtc gagcatcatc tgtgcggttt cgatatcttc ctgaacctgt tgccagtcgg
     4261 taaaacagcg cgaaacatca cttaactgcg catattcgcg tgataatgcg cgaaaacgtt
     4321 cctggtcggc gatagtttgc gcgtcaccca gcaacgcctg aacttcttca tggcgttcat
     4381 gcagggcttc cagtttggca acgatagaag gcttcatagg cgtaaatgca ccctgtaaaa
     4441 aaagaaaatg atgtactgct actccagccc gaggctgtcg cgcagaatat tcaggcgttc
     4501 gttatccccg tcacgggcgg cctgttgaag tgatttcgtt ggcgcatgga tcaagcggtt
     4561 agtcagtttc catgccagat cctgcataat ggcttgcgcg tcgccgccct gctcaagggc
     4621 cgctaacgct ttggcggtta actcatcgcg aacttgctct gcctggccgc gatactcgcg
     4681 aatggtttcg ctggcgcttt gtgctcgcag ccacgccata aattcgctgg tttcctgagc
     4741 gacaatagtt tccgcctcaa ccgctgcggc tttacgctgc gccaggttgt gcgaaatgat
     4801 gctttgcaga tcgtcaacgc tataaagata agcattcgcc agtttaccaa cttccggctc
     4861 aacatcgcgc ggaacggcaa tatccaccaa cagcattggt tggttgcgac ggctttttaa
     4921 tgcgcgctcc accatgcctt tcccgataat cggtaacggg ctggcggtgg aactgatgat
     4981 gatatcggct tcgcgcagac gttcgtcgat atcgctcagg gcaatcactt ccgcgccgac
     5041 ttcatctgcc agaatttggg cacgttcgcg agtgcggttg gcgataatca tcttctgtac
     5101 tttatgttcg cgcagatgac gcgctaccag ctcgatggtt tcgcccgcgc ctaccagcaa
     5161 cactgtgacc gtagagagcg attcaaagat ctgccgcgcc agcgtacaag ccgcaaaagc
     5221 gacagacaca gcgctggcac cgatatctgt ttcagtgcga acgcgtttcg ctacagagaa
     5281 agatttctgg aacatgcgtt ccagttcgct ggccttcata tggccttttt gcgaatcggc
     5341 aaacgctttt ttaacctgac caaggatctg cggctccccc agaaccagtg aatccaggcc
     5401 gctggcaaca cgcattaaat ggctaaccgc gtcgttatcc tgatgccagt agaggctttt
     5461 acgcagatct tcttcattaa gattgtgata atcgcaaagc cagcggatta acgcctcttg
     5521 caggttatcc tgctcttcaa cgctaaga
//
LOCUS       DAMZET010000229         5534 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.229, whole genome shotgun sequence.
ACCESSION   DAMZET010000229 DAMZET010000000
VERSION     DAMZET010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.229"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(13..1611)
                     /locus_tag="POB34_004390"
     CDS             complement(13..1611)
                     /locus_tag="POB34_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDI5921228.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGGTALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(1742..3106)
                     /gene="sdaA"
                     /locus_tag="POB34_004391"
     CDS             complement(1742..3106)
                     /gene="sdaA"
                     /locus_tag="POB34_004391"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDI5921229.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETSYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(3290..3868)
                     /locus_tag="POB34_004392"
     CDS             complement(3290..3868)
                     /locus_tag="POB34_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDI5921230.1"
                     /translation="MEYRSLTLDYFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(3872..5233)
                     /gene="pabB"
                     /locus_tag="POB34_004393"
     CDS             complement(3872..5233)
                     /gene="pabB"
                     /locus_tag="POB34_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HDI5921231.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            5307..5486
                     /locus_tag="POB34_004394"
     CDS             5307..5486
                     /locus_tag="POB34_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HDI5921232.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
BASE COUNT         1404 a         1418 c         1429 g         1283 t
ORIGIN      
        1 gatgagtaag cattaacgta acggcataat gggcgtgata tgtccattat gccgggcggg
       61 cggcggttgg ctgcccgcca gccatttggg aaaatcacgt agcggcatcg gctgtgcgta
      121 caaataccct tgcaaaatat gcacgccatg acggcgcaaa tacttcgcct gttcttgcgt
      181 ttccacgccc tccgccacca gttcaatatt cagtctttgc cccagcgcga tgatgatatc
      241 ggtcaccgtc gagttaaccg cgtcagaacc tatagctgcg gtaaatgact tatcaatttt
      301 cagcacgtca ggacgtaatg tttcaagcca ggaaaatgaa ctgttgccgg tgccgaagtc
      361 atcaatcgcc agtttgacgt ttttacgatg tagctcgcgg gcaatccgat aatcaacatc
      421 cagtaaggca tcgcgttcgg tgatttcgag gatcagttgc tgaatcgggt gagcgctaaa
      481 ccagtactga ttgagatctt ttatcagcac accacggcga aaatggctgg gtgcgacgtt
      541 aatgccaaca tgaaactgac tactcatcgg gaaaacatgg cgctgacgaa tggtttctgc
      601 catcacatag cgggtcagtg gcacaattaa atgatgttct tccgcgatag gaataaacac
      661 atccggtgaa atccagccct gacgcggatt gttccagcgc agcaaaatct ctacaccaat
      721 acattgctgg ctgcgcgcat taagcaaagg ctgacagaac aattcgaact catgttgcgc
      781 caggcccaga ttgatttcgc gggaaaagct catccggtaa gcggtcgcca gccaggcgat
      841 gtagcccacc agtaaactta gcagcaccgc cagcggtaat tgtgttggaa gatagtgcca
      901 tgccagcgcc gtaccccccg gaccattaac gttaatggta aaaggaaagc gttgcgaaga
      961 aacctgatag ttttcattgt cttcaggttg cggaagggaa tctaccagcc cattaccata
     1021 gagcaaatgc cgtttgtcca ccgtcaggct ggcgctactg atttgcggca gttgtggctc
     1081 aaggagcatt gccgtcagta agtcgatgtt aatcatctcc atgaccccag cattgctact
     1141 gcccgctgca ggcgtccatt gaatcaaaac cggactgcct ttaatcaggg cacggtcgat
     1201 cgtcaggcgt aaaagtggtt gcggtgcagg aagttcagcc agaatgtcca cgacgggcac
     1261 attgcgataa ccaaaaatgc tggagcaata aagtgtgccg tcttgcacca ggccaatgga
     1321 tcgcacagtt tggagttttg ccgcctgttt acgtaatggc aaatgggcga cagagcaggg
     1381 cagaccaatc agcggaagca agacttcgct acctgcctgt aggggaagca gtactttatc
     1441 taattcctct acagcgtgat tggcgaattg tactacccgt tgttgattta agttacgctg
     1501 tgaaataaat cgcacgctca gggtcgaagc gagcgtaacg agtgcgcaaa tcgtacaaac
     1561 aatcattcga ttacggcgat aggttttaat gatccgttgt gctttttgca tgcgggccac
     1621 cctgtaagcg tatggcgaca aacgcctgac gcaacagtgg aagtgtagtg gggaatatta
     1681 cagcagacga gaaagcgggt ataaattcgc ccatccgttg cagatgggcg agtaagaagt
     1741 attagtcaca ctggactttg attgccagac caccgcgtga ggtttcgcgg tatttggcgt
     1801 tcatgtcctt accggtttcg tacatcgttt cgatgacctt atccagcgag acgcgcggtg
     1861 cactggtgcg gcgcagagcc atccgtgcgg cgttaatcgc cttcacagag gcaatggcat
     1921 tacgctcaat gcacggcacc tgaacctgcc ctgcaaccgg gtcgcaggtt aaaccaaggt
     1981 tgtgttccat gccaatttcc gccgccacgc aaacctgttc cgggctaccg cccagcagtt
     2041 ctgcaagacc cgcagcagcc attgaacagg caacacccac ttcgccctgg caaccaactt
     2101 ccgcaccgga aatagaggcg ttcattttat acaatgcacc aatcgcgccc gctgccataa
     2161 agtaacgggt atagatgtcc gggctgaccg attcaataaa gtggtcatag taagccagca
     2221 ctgccggaac gataccgcag gcaccgttgg ttggcgcagt taccacacga ccaccggcgg
     2281 cgttttcttc gttaactgcc agcgcaaaca tgtttaccca gtcaatgaca ttcatcggat
     2341 cgttagacag tttatcgctg gaaactaaca tccggcgcag ggcagacgca cgacgtggca
     2401 cgcgcagcgg gcctggcagt acaccttcgg tgttcatccc gcgatcgata catgcctgca
     2461 tggtttgcca gacatgcgcg aaatactcgt cgatctcttt cttgctgtgc agcgccagtt
     2521 cgttctgcat agcgagacca gacagcgaat agctggtttc attacagtac gcgagcagtt
     2581 cggtggcaga tttgaacgga tacggcacgc tcacttcgtt ggcagcatcc tgaccaaagt
     2641 gttcttcatc gacgataaaa ccgccgccga tggaataata agttttgctg tagacgactt
     2701 catcgccgtt ataggcgtgg atttgcatac cgttttcatg cagcggcagg ttgccgttat
     2761 gaaaacgcat cccgttgtcg cgcgggaaat ccacttcatg ccgtccctgt gccagcagca
     2821 gacgttcgcg ctcttctacg tcgcgaataa aaccgggaat actgtcgata tccacggtgg
     2881 caggttcgtt acctgcaaga cccataataa tggcgatatc ggtgtggtgg cctttacccg
     2941 tcagcgacag tgaaccataa acgtccacgg caacgcgagt aacgctatcc agtaagcctt
     3001 tttcgaccag atcatcgacg aactgtttac ccgccttcat aggccctacg gtatgggaag
     3061 atgagggacc aatccccacc ttaaacatgt cgaatagact aatcacgata atactcctga
     3121 caaggtgact ggacttccag taacgaacta ataacagcgc atagtgtaag agggaacgcg
     3181 gcgactggct taactattca catgaattaa actaatcaaa aaccgcgatt taactaatat
     3241 tgcgatgatt gtctcaagta gtgttttgta gatgtaaaga taagtatagt cagggtttca
     3301 caccaatttg cagcgccagc tcacgaatta tgcctgcggt cattccccat acaaaatact
     3361 gttcgtacca ggacagccat acccgatgtg aatcgccacg gcggtagata tctaaagggt
     3421 gataacgacc cagatgtaat gcctgggcga gcggcatttc aaacaccgcc gagacttcat
     3481 cttcactggc gcgatacggc agatcgggcg ggataatgcc gaccactggg gttacctgat
     3541 agccagttac gctatcgacg ggcggcagca cgccgataac ttcaacggcg gaaggcggta
     3601 tagcgacctc ttcttcagct tcgcgcagcg cggcggcgat aactgatgcg tccgtgtcat
     3661 cgactgcacc tccagggaat gccacttgtc cagcgtgttt acgcagatga attgaacgct
     3721 gagtcagcaa caaccccggt tgcggtcgac ggacgatggg gattaacacg gcagcctgac
     3781 gatgatttag ggtttcccgg ttaatttgcg ggcgcaaaag ttgaaagcga gataaaaaat
     3841 aatcaagcgt caggctacgg tattccacgt cttacttctc cagttggcgt aatatcttat
     3901 taactttatc aaaagtttcc tgatattccg cttcttcctg gctatcggcg acaattccac
     3961 cgcccgcaga gcagtatatt tgtccgttaa tggcagtcag cgtgcggata gtaatgctgg
     4021 tatccatgtt gccgcaaaag ctcaaatagc caatgctgcc gcaccaggca ttacgtcgct
     4081 gcggttccag ttcgtcgata atttccatag cccgtacttt cggagccccg gttattgagc
     4141 caccaggaaa agctgcgcgc agcagatcgc tggcgtgtaa ctgttctggt agtcgcgccg
     4201 ttatagtgct gaccagatga tgcacggcag ggaagggttc caccacgaag agctctggta
     4261 cttttaccga accggctacg gcaacacgac cgatatcatt acgcattaaa tcgacaatca
     4321 tcagattttc ggcacgatct ttcgctgagt tcgccagttt ttctgcttgt ttgctatctt
     4381 cctgaggatc gggcaggcgt ggtagcgtgc ctttaatcgg gcgggtctgg atttcactat
     4441 tatcacaaag aataaaccgc tctggcgaaa ggcttaaaat tgcaccctgt tcaagacgta
     4501 aaaaagcgct aaatggcgcg cggttggcct gattaagctg aaggaatgcc tgccattcat
     4561 cgccagaata ggtcgcatga aaacgctggg cgagattcac ctgatagcaa tcaccgctgt
     4621 gcagatattc ctgtacctgg cgaaattttt cgccgtactg ctcgcgggtc atattggatt
     4681 gccagtcgga agtgagcgtg aaatcttcct gcggcgagaa ttgctggctt tccagccagg
     4741 cccgacgggc attgacatca ttatgactca gcaaagaaac tgtatgacgc tggtggtcga
     4801 caatgagcgc ccaatcgtag atacccactg ccatatccgg cagaacgata tcttgttccg
     4861 caatttctgg cagtgactca aaacggcggc ccagatcgta gccaaacaac cccagtgcgc
     4921 cgccctgaaa tggcaaatct tcgttatgcg ttgggcgaat gtctgcgcga tccagcacct
     4981 gctggagcac ctgtagcggg tcatcagtgg tcgttgtgcg tttttcgctt tcactaacaa
     5041 cggtttcttt accgaaagtg gttaaagtgc aaatcggatc ggcgaccaca atatcaaagc
     5101 ggctatacgg atgatcggca tagccggagt gtaaaagcat cgcccacggc aggtggctta
     5161 agcgggagaa ataaaattca gcggcgtcct gacgccaggg taaagtaatc acagcgggag
     5221 ataacgtctt catcagtcct gactctactg gctatgtgcc gggtagcatg aaataattag
     5281 cgccaacaat ttagcaggag ttaacaatgt ttgcaggttt accttcactc acccatgaac
     5341 agcagcaaaa agctgtcgag cggatccagg aactgatggc acaagggatg agcagcggcc
     5401 aggcaatcgc gctggtggca gaagagctgc gtgccaacca ctccggcgag cgaatcgtgg
     5461 cgcgttttga ggatgaagac gagtaacaca ctaatccgta tcgatttcgt tatagaagcc
     5521 gatgatcatt tgat
//
LOCUS       DAMZET010000230         5493 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.230, whole genome shotgun sequence.
ACCESSION   DAMZET010000230 DAMZET010000000
VERSION     DAMZET010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.230"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..2118
                     /locus_tag="POB34_004395"
     CDS             <1..2118
                     /locus_tag="POB34_004395"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDI5921233.1"
                     /translation="DGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWE
                     WELKPNVFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFK
                     LEFRITVKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHIT
                     LDSIGEAVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENI
                     YSADTSRSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLR
                     QLSYSASHDALTHLANRASFEKQLRMLLQTVNSTHQRHALVFIDLDRFKAVNDSAGHA
                     AGDALLRELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYH
                     FIWEGRVHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAA
                     HSERAAMSLDEQWRMIKENQLMMMAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQK
                     FRRSFSDPALSHALDRRVFHEFFQLAAKAVASKGISIALPLSVAGLSSATLVNDLLEQ
                     LENSPLPPRLLHLIIPAEAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMAD
                     YLLLDGELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLI
                     YGDVIADAQPLDLLVNSSYFAIN"
     gene            complement(2157..3014)
                     /gene="alkA"
                     /locus_tag="POB34_004396"
     CDS             complement(2157..3014)
                     /gene="alkA"
                     /locus_tag="POB34_004396"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HDI5921234.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVAEDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            3148..4500
                     /gene="yegD"
                     /locus_tag="POB34_004397"
     CDS             3148..4500
                     /gene="yegD"
                     /locus_tag="POB34_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDI5921235.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(4512..>5493)
                     /gene="yegI"
                     /locus_tag="POB34_004398"
     CDS             complement(4512..>5493)
                     /gene="yegI"
                     /locus_tag="POB34_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HDI5921236.1"
                     /translation="DCPWCALDNQGVIYFIDLGEEVITTGGDFVLAKVWAMVMASVAP
                     PALQLPLPDHFQPTGRPLPLGLLRREYIILLEIALSALSLLLCGLQAEPRYIILVPVL
                     AAIWIIGSLTSKAYKAEVQQRREAFNRAKMDYDHLVRQIQQVGGLEGFIAKRTMLEKM
                     KDEILGLPEEEKRALAALHDTARERQKQKFLEGFFIDVASIPGVGPARKAALRSFGIE
                     TAADVTRRGVKQVKGFGDHLTQAVIDWKASCERRFVFRPNEAITPADRQAVMAKMTAK
                     RHRLESTLTVGATELQRFRLHAPARTMPLMEPLRQAAEKLAQAQADLSRC"
BASE COUNT         1292 a         1441 c         1496 g         1264 t
ORIGIN      
        1 gatggcacgc cgctctattt tatcgctcag attgaagaca ttaacgagct aaaacgcacc
       61 gaacaggtca atcagcaact gatggagcgc atcacgctgg ctaacgaagc gggcgggatt
      121 ggcatctggg agtgggagtt gaagccgaat gtttttagct gggataagcg gatgttcgag
      181 ctgtatgaaa ttcctccaca tatcaaaccg aactggcagg tgtggtacga gtgcgtgctg
      241 ccggaagatc gccagcatgc cgaaaaagtg attcgtgatt cgttgcaatc acgctcgccc
      301 tttaaactgg aatttcgcat taccgtaaaa gacggtattc gccatatccg cgccctcgcc
      361 aaccgggtac tgaataaaga aggcgaagtc gaacgtctcc tcggcatcaa tatggatatg
      421 accgaagtga aacagcttaa cgaggcattg tttcaggaaa aagagcgcct gcacattacg
      481 cttgattcca tcggtgaagc cgtggtctgt attgatatgg cgatgaaaat tacctttatg
      541 aatccagtgg cggagaagat gagcggctgg acgcaggaag aagcgttagg tgttccgctc
      601 ctgacggtgt tgcatattac ttttggcgac aacggaccat taatggagaa catttacagt
      661 gccgacacct cacgttccgc aatcgaacaa gatgtggtgt tgcactgtcg gagcggcggc
      721 agttacgacg tgcattacag tattacgccg ttaagtactc tggacggcag caatattggt
      781 tcggttctgg tgattcagga cgtcaccgaa tcacgcaaaa tgctgcgcca gctgagctac
      841 agcgcctccc atgatgcact gacgcatctc gccaatcgcg ccagttttga gaagcaactg
      901 cgtatgctgc tgcaaacggt aaacagtaca catcagcgac atgccctggt gtttatcgat
      961 cttgatcgct ttaaagcggt gaatgacagc gccgggcatg cggcgggcga cgctttgctg
     1021 cgcgaactgg cgtcgttgat gctgagtatg ctgcgttcca gcgacgtgct ggcgcgactc
     1081 ggtggggatg aatttggtct gctgctacca gactgtaatg ttgaaagcgc gcgttttatc
     1141 gctacacgca ttatcagtgc cgtgaatgac tatcacttta tatgggaagg ccgtgtgcat
     1201 cgggtaggtg ccagtgccgg gattaccttg attgatgaca acaatcatca ggcggcagaa
     1261 gtgatgtcgc aggctgatat cgcctgttat gcctccaaaa atggtggacg gggccgggtg
     1321 acggtttacg aaccacagca agctgccgca catagcgaac gggcggcgat gtcgcttgat
     1381 gaacagtggc ggatgattaa agagaatcag ttgatgatga tggcccacgg tgtcgcttcg
     1441 ccgcggatcc cggaagcgcg taatttgtgg ctgatttcgc ttaaactctg gagttgcgaa
     1501 ggcgagatta ttgatgaaca aaaatttcgt cgtagcttca gcgatccggc acttagccat
     1561 gctcttgacc gacgggtatt ccacgaattt ttccagctgg ccgcaaaagc ggttgccagt
     1621 aaaggcataa gcatcgccct ccccctttcc gttgccggtt tgagtagcgc cacgctggtg
     1681 aatgatctgc ttgagcagct ggaaaatagc cctctaccac cacggttatt acacctgatt
     1741 attccggctg aagcgatttt agatcacgca gaaagcgtgc aaaaactgcg gctggcggga
     1801 tgtcggatag tgctcagcca ggtgggccgc gatctgcaaa tcttcaactc gctgaaagcg
     1861 aatatggcag attacctgct acttgatggt gagttatgcg ccaacgtgca gggtaatttg
     1921 atggatgaga tgctgattac gattattcag gggcacgctc agcgactcgg gatgaaaacc
     1981 atcgccgggc cagtcgtttt acccttagtg atggatacgc tttctggcat cggcgtcgat
     2041 ctgatttatg gtgatgtgat tgccgatgcc caaccgctgg atttgctggt gaatagcagt
     2101 tatttcgcga ttaactgagg gagcgtttgc ccattgcctg atgcgacgta tcaggcctac
     2161 aattcgtccg ttccgtctgg ttgccagcct tccgtatacc agatatgcaa cagcgcataa
     2221 gaacgccagg gcttccagcg ctcggcataa cggcggattt gtgccggtgt catccccgga
     2281 aatcgctgtt taatcagata atcatccggc agaaaaacat ctttcgcctg ccagccacgc
     2341 aaagcgaaat aattcgccgt ccagcgcccg atacccggaa aagtttgcag cgttttcatc
     2401 gcctgctcca catcgcccgg aattgtcatt ggtaagctgc cctccagcgc cgcatttgcc
     2461 agatgaatca gtgcctctgc ccgtttcaac ggcatgccta acgcttttaa tgcctgcggg
     2521 tcggctgctg ccagccgctg aggcgtgggg aagcagatat actccggaaa atcatccagc
     2581 cgttcgccat aaagctgtac aactttggca gtcaattttg ccgccatcgc cacgctcacc
     2641 agttggccta aaatcgcccg cacaccctgc tcaaaagcat caatacagcc gggtaaacgc
     2701 aatccgggcc gcgccgcgcc taacttgccc aacgcaccgt taacaatctg cgggttacat
     2761 tgcagatcaa acaggcggct cattttcgcc agacactcag cggcaacggg ttctaaacct
     2821 gcacttaaat ttatgtgcag agtatggcgg gctatatccg gaatagcagt caccacgccg
     2881 cgatattcgc ccaccgccag actacgggca taataatcct cagcgaccgt ttccacacca
     2941 ctcaccgcac gggcggcgag aaatcccaac atccacgacc agtcatacgg cggctgccag
     3001 ttcagggtat acatcgcatc tccttattca tccgctttca gcataaacgt tattcagacg
     3061 ctacgctttg ctttcatatt ccggttgtcg cgacggcaac atttcgctaa agtcacgccc
     3121 cttcttcacc ggcatgggga ttaattcgtg tttattggtt ttgattacgg tacagcaaac
     3181 tgttcagtgg cggtcatgcg tgacggtaaa ccgcaattac tgaaaatgga aaacgacagc
     3241 acgctgctgc cttcaatgct ttgcgcgcca acgcgtgaag cagtaagtga atggctgtac
     3301 cgccatcatg atgttccggc agacgacgat gaaacgcagg cgctgttacg tcgggcgatt
     3361 cgttataacc gcgaagaaga tatcgatgtt acggcgaaaa gcgtgcagtt cggtctttcc
     3421 tcactggcac agtacattga tgatccagaa gaagtgtggt ttgtgaaatc accaaaatcg
     3481 ttcctcggtg ccagcggctt aaaaccgcag caggtagcgc tgtttgagga tctggtctgc
     3541 gcaatgatgt tgcacattcg ccagcaggcg caggcgcagc tgccagaagc gattactcag
     3601 gcggtgattg gtcgcccgat caacttccag gggctgggcg gcgatgaagc aaacgcccag
     3661 gcgcaaggga ttctggaacg cgcggcgaag cgtgccggat tcagggacgt ggtattccag
     3721 tacgagccgg tcgcggctgg gctggattac gaagccacct tgcaggaaga aaaacgggtg
     3781 ctggtggtgg atatcggcgg tggtacgact gactgttcat tgctgctgat ggggccgcag
     3841 tggcgttcgc gtctcgatcg tgaagccagc ctgctgggtc acagtggttg ccgtattggc
     3901 ggtaacgatc tggacatcgc actggcgttt aaaaacctga tgccgttact gggcatgggt
     3961 ggcgaaaccg aaaaaggcat cgccctgccg attctgccgt ggtggaatgc ggttgccatc
     4021 aacgacgtac ctgcgcagag tgatttctac agtagtgcca acggtcgtct gcttaacgat
     4081 ctggtacgcg atgcccgcga accggaaaaa gtggccctgc tacagaaagt ctggcgtcag
     4141 cgtttaagct atcgcctggt gcgtagcgca gaagagtgca aaattgctct ttcaagcgta
     4201 gcggaaaccc gcgcctcact gccgtttatc agcgatgaac tggctacgct gattagccag
     4261 caagggctgg aaagcgccct cagccagcca ctggcgcgga ttctggaaca ggtgcaactg
     4321 gccctggata acgcccagga aaaaccggac gttatctacc tgaccggcgg tagcgcccgt
     4381 tcgccgctga ttaaaaaagc gctggcagaa cagttgccgg gaattccgat tgcaggcggc
     4441 gatgactttg gctccgtcac cgccgggctg gcacgctggg cggaagtggt gtttcgttaa
     4501 aagaaaatgg ctcagcagcg gcttaaatct gcctgcgcct gagccagttt ttctgccgcc
     4561 tgacgtaaag gttccatcaa cggcatggtc cgtgctggag catgaaggcg gaatcgctgc
     4621 aactctgtcg cgccgacagt caacgtcgat tccagccgat gtcgtttggc ggtcattttc
     4681 gccattaccg cttgtctgtc tgctggcgtt atcgcttcat tcggcctgaa cacaaaacgg
     4741 cgttcacagc tcgctttcca gtcgataacc gcctgggtca gatggtcgcc aaacccttta
     4801 acttgcttaa cgccacgacg ggtaacatct gcggctgttt caataccaaa agaccgtaac
     4861 gccgctttac gcgcagggcc aacgccggga atagaggcaa catcaataaa aaatccctcc
     4921 agaaacttct gcttctgccg ttcccttgca gtgtcatgaa gtgctgccag agcgcgtttc
     4981 tcttcttccg gtaacccgag aatttcgtcc ttcatttttt cgagcatcgt ccgtttggcg
     5041 ataaaacctt ccaggccgcc tacctgctgg atctggcgga ctaaatggtc atagtccatt
     5101 ttcgcacgat taaatgcctc acggcgttgc tgaacttctg ctttgtacgc tttgcttgtc
     5161 agactgccaa taatccagat agccgccagc acaggaacca aaataatata acgcggttct
     5221 gcctgaaggc cgcaaagcag cagcgataat gctgacagtg cgatctcaag cagaatgatg
     5281 tattcgcgcc gtaacaggcc taaaggaagc ggcctgccag tcggttggaa atgatcgggt
     5341 aatggcagtt gcaatgctgg cggtgctact gacgccatca ccatcgccca gacttttgcc
     5401 agcacaaaat ccccgccggt ggtaatgacc tcttcgccga gatcaataaa atagataaca
     5461 ccttgattat ccagcgcaca ccatgggcag tca
//
LOCUS       DAMZET010000231         5486 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.231, whole genome shotgun sequence.
ACCESSION   DAMZET010000231 DAMZET010000000
VERSION     DAMZET010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.231"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..751
                     /locus_tag="POB34_004399"
     CDS             <1..751
                     /locus_tag="POB34_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDI5921237.1"
                     /translation="NSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQ
                     HLSSAGEWDISGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVA
                     DIPVQGNLDYTNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIG
                     YKSLASRSGKDVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQK
                     FTVVWGDSQHCSLHLPEHMEDTANRLILPCH"
     gene            766..1497
                     /locus_tag="POB34_004400"
     CDS             766..1497
                     /locus_tag="POB34_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDI5921238.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            1494..2555
                     /locus_tag="POB34_004401"
     CDS             1494..2555
                     /locus_tag="POB34_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI5921239.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            2707..3753
                     /locus_tag="POB34_004402"
     CDS             2707..3753
                     /locus_tag="POB34_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDI5921240.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(3750..4541)
                     /gene="nei"
                     /locus_tag="POB34_004403"
     CDS             complement(3750..4541)
                     /gene="nei"
                     /locus_tag="POB34_004403"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HDI5921241.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(4577..5311)
                     /gene="pxpA"
                     /locus_tag="POB34_004404"
     CDS             complement(4577..5311)
                     /gene="pxpA"
                     /locus_tag="POB34_004404"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDI5921242.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMHACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEQQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(5301..>5486)
                     /locus_tag="POB34_004405"
     CDS             complement(5301..>5486)
                     /locus_tag="POB34_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921243.1"
                     /translation="QTTGGYPRIACIIEADMYHLAQIPLGQPIHFVQCSLEEALKARQ
                     DQQRYFEQLAWRLHNEN"
BASE COUNT         1292 a         1441 c         1383 g         1370 t
ORIGIN      
        1 aaacagtagc cacagcacca cacaccgcat gtcgtggaac gatacgctgg atgaacgtaa
       61 tagctggggc atgtctgccg gactgcaatc cgaccgtccg gacaatggag cccaggtgag
      121 cggtaactat cagcatctga gttcagcggg tgagtgggat atttctggta cctatgccgc
      181 caatgattac agttccgtca gcagcagctg gagcggttct ttcaccgcaa cccaatatgg
      241 tgcagcgttt catcgccgca gctccaccaa tgaaccacgc ctgatggtca gcaccgatgg
      301 cgtggcagat attccggtcc agggcaatct cgactacacc aaccattttg gcattgcggt
      361 ggtgccgttg atttccagtt accagccttc caccgtggcg gtgaacatga atgacttacc
      421 cgacggcgta acagttgcag aaaacgttat caaagaaacg tggattgaag gcgcgatagg
      481 ttacaaatca ctggcttccc gttccggtaa agacgttaac gtcatcattc gcaacgccag
      541 cggtcagttt cctcccctcg gagcggatat ccgccaggat gacagcggca ttagcgtggg
      601 gatggttggc gaggaaggac atgcctggtt aagcggagtc gctgaaaatc aaaagtttac
      661 cgtggtctgg ggtgatagcc agcattgctc gctccatctt cctgaacata tggaagacac
      721 cgcaaatcgc ctgattttac cttgtcatta attaaggaaa tgaaaatgac atttatgaaa
      781 ggactacccc ttttgttgtt ggttgccagt ctgtgcagcc acgctgcact acaacccgat
      841 cgcactcgta ttgtgtttaa cgctaatgat aaagctacca gtctgcgggt ggataatcgt
      901 agcgataaac tcccctacct cgcttattcc tggctcgaaa atgaaaaagg ggagaaaagc
      961 gatgatcttc tggtcgccct gccgccgata caacgtctgg aaccgaaagc aacaacgcag
     1021 gtacggatag taaagcaagc ctcgaccaca aaattaccgg gcgatcgcga aacgctattt
     1081 ttctacaata tgcgtgaaat tcctcccgcc ccggaaaaaa acagcgacca tgccgttctg
     1141 caagtcgcca ttcagagtcg tatcaaggtg ttctggcgac cggctgcatt acgcaagaaa
     1201 gcaggagaaa aggtcgaact gcagttacag gtcagccagc agggcaacca actgacactg
     1261 aaaaatccta ccgcgtatta tctgaccatt gcctacctgg gacgaaatga aaaaggcgtt
     1321 ctccccggct tcaaaaccgt gatggttgcg ccttttagta cggtcaacac aaacaccgga
     1381 aattatagcg gcagccagtt ttatctcggt tatatggatg attacggcgc gttacgcatg
     1441 acgacgctca actgcagcgg acaatgccat ttacaagccg tggaggcgaa gaaatgagtg
     1501 ctggcaaggg agtgttgctc gtcatctgtc tgttatttct gccgttgaag tctgcgcttg
     1561 ccctgaactg ctactttggt tcatccggtg gctcggtaga aaaatcagaa gcgattcaac
     1621 cttttgctgt accaggcaat gccaaactcg gcgataagat ttgggaatct gacgatatta
     1681 aaattcccgt ctattgtgac aacaatacca acggcaattt tgaaagtgag catgtttatg
     1741 cctgggtaaa cccctacccg ggggtgcagg atcgctatta tcaactcggc gtaacttaca
     1801 atggcgttga ttatgacgcc aaccagggaa aaagccgcat cgacactaac cagtgtattg
     1861 acagtaagaa cattgacatt tatacccctg agcagattat cgcgatggga tggcagaaca
     1921 aaatttgctc gggtgatccc gctaatattc acatgtcgcg tacctttctc gctcgcatgc
     1981 ggttatacgt caaaatacga gaaatgccgc cgcatgatta tcaaagtacg cttagcgact
     2041 atatcgtcgt gcaatttgac ggtgccggta gcgttaacga agatcctact gcccaaaacc
     2101 tgaaatatca tattaccggt ctggaaaaca ttcgcgtgct ggattgcagc gtcaattttt
     2161 ccatttcacc ggaaacacag gtgattgatt ttggtaaatt taatttgctg gatattcgtc
     2221 gccacacaat gtcgaaaaca ttcagtatta agacgacaaa atcacaaaat gaccaatgca
     2281 ccgacggatt taaagtcagt tcctcctttt ataccgaaga aacgttggtt gaagaagata
     2341 aagcattgtt gattggtaat ggtctgaagc tacgtttgct ggatgaaaac gcctcgcctt
     2401 ataccttcaa taaatatgct gaatacgccg atttcaccag cgacatgttg gtctacgaaa
     2461 aaacctatac agctgaactt tcgtccattg caggtacccc catcgaggct gggcccttcg
     2521 atacagtggt gctttttaaa attaactata actgatttgc catgatgccc tttgcagccg
     2581 gaaagggcat ctctttcaga aaattgctga ttttcccctt ttccttgatt gctgttattt
     2641 tttcctggcc cggctttctt tatgctgtat cgatacacca aagagaaaat aatgagggag
     2701 cgaaggatgc cagttttgca gtggggaatg ttatgtgtgc tatcacttct cctttctatt
     2761 ggtttcctcg cggtacatct cccggcggcg ctattacttg ggccgatgat cgccgggatc
     2821 atcttcagta tgcgcggaat tactctgcaa cttccccgct ccgcttttct cgccgcgcag
     2881 gccattcttg gctgcatgat tgcgcaaaac ctcactggtt cgattctcac cacgctggcc
     2941 gccaactggc cgatcgtgct tgctatttta ctggtgacgc tgctttccag cgccatcgtg
     3001 ggttggttat tggtgcgcta tagctcactg cctggaaata ccggtgcctg gggttcttct
     3061 ccaggcggcg cagcggcaat ggtcgctatg gcgcaagatt acggcgcaga tattcgcctg
     3121 gtggcgttta tgcaatatct gcgagtgctg ttcgtcgcgg gggccgctgt tctggtcacc
     3181 cgcatgatgc tgggtgataa cgctgaagcg gttaaccagc aaatcgtctg gttcccgcca
     3241 gtgagcatta atctcctgct tacgattttg ctcgcagtcg ttgccggtac ggcgggatgc
     3301 atgctgcgtc ttccttccgg cacgatgctc atcccgatgc tggcgggcgc agtgctccag
     3361 tctggtcagc tcatcaccat cgaactaccg gaatggctgc tggcgatggc gtatatggca
     3421 attggctggc ggattggtct tggtttcgat aaacaaatct tactgcgggc actacgccca
     3481 ctaccgcaaa tcctgctgtc gatttttgct ctgctggcta tttgtgcagg tatggcgtgg
     3541 ggactgaccc ggtttatgca tattgatttt atgactgcct acctcgccac cagtcccggc
     3601 gggcttgata ccgtggcggt catcgccgca gggagcaatg ccgatatggc gcttattatg
     3661 gcgatgcaaa ccctgcgcct gttcagtatt ttgctgacgg ggccagccat tgcgcggttt
     3721 atttcaacct atgcgccgaa gcggtcggcc tagtgctggc agcctggaca ccagtaaaac
     3781 gggcgcgagg acaacgtggt tttctcaatg atgccgccac agcgttcgca cagttcgcca
     3841 tcacgatgaa aaaccttaaa gcgaaacagc gccccatgat gcttattctc atccacctgc
     3901 ccccgcgtag cgtaagaaaa ccgaggaatc tccagtaacg cgtgtgcgag tgcatccagt
     3961 tgcgccgcat tgagatcttt cgctttatga tttccagtca acccaacctg ccagaggatc
     4021 tccacccgca aataattgcc aagcccagcc agaaacgcct gatcgagcag taatcctgca
     4081 aactgacggt tacgaaagcg cggtgacaat aaccgttcct ttaccacctc cggcgtcaga
     4141 ttcggatcca gcacatcggg accaacgcgt tgtaaaaacg gatgcgtggt cagttgttcc
     4201 ggggtcaaca tctcaatatc cgaggcgcta taaagcagaa tggttttgtc agccgtttgc
     4261 agttttaccc gcaatactcg cgtggtctgc ggctcttcgc cggtatcaac cacgcgccag
     4321 acgccgtaaa gctgattatg gctgtagagc gttaagtcgt tggaaaaatg agttaacaac
     4381 gccttaccac gcgtttccac atgggtaacg tgttgaccga taagttgtga ttgataagtt
     4441 tttaactgcg ggaaggcaaa ccagacatca gttagtggtt tgcctttgat cgccgcctcc
     4501 aggttatccg ctgcacggcg gatctccggg ccttcaggca tggtgatatc cttttttcat
     4561 tctttattaa gcggggttat gctgcgacaa caatcccctt tttggcaaat gtagctcgta
     4621 atcggcgggc gaacgccaga gcgtgctcgc cgtcgccatg caggcagacc gtttgcgccg
     4681 cgaccgttgc ccattcgccg gtgatacttt tgactctgcc gtgttgcacc atctccagcg
     4741 tttgcgccag cgcctgttgt tcgttttcaa tcaacgcgcc tcgctgactt cgcggcacca
     4801 gcgagccgtc agcctgataa ccgcgatcgg caaacacttc ctcgcgcgtt gtcaggccat
     4861 attgcttgcc tgcacgaatc agctcgcttc ctgccagccc aacgagaatc aatgccggat
     4921 cgcaagcgta taccgctctg gcgatggcgt ctgccagttg tgcttctttc gccgcctggt
     4981 tgtacaacat gccgtgcggt ttgacatgac gcattacgcc gccttgcgca cgggcaatcg
     5041 ttgccagcgc gccaatttga tacagcgtct gggcgtaaac ggtttctggc ggcagctgca
     5101 tggcgctgcg accaaaattt tccctgtcgg gaaaactcgg gtgagcgcca attgcaacac
     5161 cattttttat tgcttcacgc acgcaagcat gcatggtttg cgcatcgcct gcatgaaatc
     5221 cacaggcaat attggcagag gaaaccagcg ttaatagctc tgcgtcgctg gcgcagcctt
     5281 cgcccagatc ggcgttcagg tcaattttca ttgtgcagcc gccacgctaa ttgttcaaaa
     5341 taacgttgct gatcttgccg cgctttcagt gcctcttcca gtgaacactg gacaaaatga
     5401 atcggctgac cgagcggaat ttgcgccaga tggtacatat cagcctcaat gatgcaggca
     5461 atacgcgggt aaccaccggt ggtctg
//
LOCUS       DAMZET010000232         5485 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.232, whole genome shotgun sequence.
ACCESSION   DAMZET010000232 DAMZET010000000
VERSION     DAMZET010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.232"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(13..870)
                     /gene="murI"
                     /locus_tag="POB34_004406"
     CDS             complement(13..870)
                     /gene="murI"
                     /locus_tag="POB34_004406"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDI5921244.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(815..2659)
                     /gene="btuB"
                     /locus_tag="POB34_004407"
     CDS             complement(815..2659)
                     /gene="btuB"
                     /locus_tag="POB34_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HDI5921245.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHFGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     regulatory      complement(2724..2914)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3028..4128
                     /gene="trmA"
                     /locus_tag="POB34_004408"
     CDS             3028..4128
                     /gene="trmA"
                     /locus_tag="POB34_004408"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HDI5921246.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4168..4527)
                     /locus_tag="POB34_004409"
     CDS             complement(4168..4527)
                     /locus_tag="POB34_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HDI5921247.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4527..5177)
                     /gene="fabR"
                     /locus_tag="POB34_004410"
     CDS             complement(4527..5177)
                     /gene="fabR"
                     /locus_tag="POB34_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HDI5921248.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
BASE COUNT         1450 a         1420 c         1327 g         1288 t
ORIGIN      
        1 cattcaacca aatcagccta aaactgccag tttttcgagc gtttcgaagc cgtaacgctg
       61 taaaacgggc aataattgtt cagcttctgg cgtcattgcc atacaaaagg caatattcgc
      121 atcggcagat tttgcatccg gagcttcatg ttctaacagc caggccgttc tgcgagcaat
      181 cgctgcgcca gaatccacca gccgggttcc ctctggcagc acctgtaaga gttcttcttg
      241 tagtagaggg aaatgggtgc aacccaatac aacggtatct ggcggctctt tcattcttaa
      301 ccacgggcgt aggatacgtt ttagtgcatc cagagaaaca tcttcgccat gtagcttcgc
      361 ttcagccaac tcaaccatct ctgccgagcc cagcatttct atctggcatt cattagcgaa
      421 acgcgcgatc agctcatgag tataagaacg tttaacagtt ccgcgggttg ccagtaatcc
      481 gacaatgcca tttgccgtca gacgtgcagc aggtttaatc gccggcacga caccaacaac
      541 cgggaagtcg aacttttcgc gtaatgcagg aagtgaaacg gtactggcgg tgttgcaagc
      601 gaccacagcc agcgcaaggg gataacgttc ttgcactgcg gtgacaattg ccaccactcg
      661 ctcaacaata aactcttcgc ttttttcgcc atacggaaaa gcgacgttat cgaaagcata
      721 aatgtaatgg agatccggta agagatgccg gatctcgtca tagaccgaca acccaccgac
      781 gccggagtca aacaccagca cggtgggacg tggttcagaa ggtgtagctg ccagacaagg
      841 tgtattcccg tcctgcagtt tggtagccat agactgtctc ataatctttg tcgaacaggt
      901 tggctatttt accacgaact gtcaggtgag aggtgaccgg atacgcaacc gcaagatccc
      961 acaagctcac accgcccatt ttaacggttt gataaggata agatgagtaa tccttatcat
     1021 agcgagtgcc taaatactga taagtaatac cccagtcgaa gtcatacaac tgccagtcga
     1081 gctggtattt cacctgctgt ttagcacggc gtaacaacgg cgtgtcggta atcgcattgc
     1141 gcgcatcgac ataatcataa ctcacagtat gcgtcagtgg tccggtatca aaattggcgg
     1201 tcgcctcgac gcccttaatc cgcgctttcc cttcgttgta atatttcagg gtgtgatcat
     1261 cataatcgat caagtcactg acatcgttac gatatccgga aatacgccag ttcaccccag
     1321 cggttaagcc ttcaaacgcg ccttcccact gtttgctttt ctccgggtcc agattcggat
     1381 ttccgtagaa gccatacagt tgccccagat ttggtgcctt ataagatgtc ccgtaggaag
     1441 caatgaagcg ataaccttcg atgaattccc aaccggcgct ggtttgccag gttccatgac
     1501 gaccaaactg tgagttatcg tcactgcgtg ctgcgccttc aaaggtaaaa tcgccgactt
     1561 gttgcagccc ggtcagatag atgccggtat tacgttgatc atatccattc tcaacataac
     1621 ctgtacccgg cgtcgtagtc tgtttctgcc agtcgacacc cgcaccaata ctaccgtgac
     1681 caacgatgac attgtttgcc cactggacgg tgtattgctt catctcatcg agcgtcgccg
     1741 acgaatcata acgaccaaaa tggggatcgt agttgtaatc tttgctatgg ctatagctgg
     1801 taatgagttg tgatttaatc agttcgccgt tatagcgcag cccggcgtcc caactttggc
     1861 tatagagttt acgggtatcg agcaacggtg aaccgggaga ataatacgcg tcataattgg
     1921 tacggttatc atagccatag ccgcgcacaa agccgctcca ggcatcagta aagttatgct
     1981 ccagcgcgcc ataaagcgtt ttacttaaaa aaccatcgtt atctgtctgc gcttgcgttc
     2041 cggtattacc ataggcaaca acatcataac catgagtatg ggcataatcg cccaacagcg
     2101 ttacccgtgt cttatccccc agttgttgct gcgtagagac atcatagttc tgataactat
     2161 tgcttcccca ccctgctgaa atttccgttc cgggttcatc gcgcgtcgtg atgatattca
     2221 ccaccccgcc tattgcatcg gaaccataaa cagcggagcg cggcccacgg atatattcaa
     2281 cacgctggac aagcgcaata gggaactggc taaggtcggc agaaccactc acccccgcca
     2341 gattcaggcg tacgccatca attaacacca acacatgact ggcatttgta ccgcgaataa
     2401 aaatagatga gagctgacct gaaccgccgt tttgggtgat atcgacgccc ggaagacggc
     2461 gcagcacatc attgaccgag gtcgactgcc agcggtcgat atcctgacgg gtcacaacgg
     2521 tggttggtgc aagcacagtg ctgcgcggct gttcaaaacg gttagcagta acgacgagag
     2581 tatccgggct ggtatcctgt gcccaagcgg aaaaggctgt gacagaacac gccgtcagca
     2641 gcgaagcttt tttaatcatt gtaaagcatc cacaatagaa gaaggatgcc gcaggtttca
     2701 tcaatattac gcgatgatga gaaccagatg cgacgttggc cggcaggtct tcgggcttgg
     2761 aggggtatct aagatactaa gagatgatga cttcccaccg gatggcagtg tccgcataac
     2821 gcaatcatcg cacctttcct taccgctgcg cgtcagctcc agattcgtac tggattccct
     2881 attaactcac aggaccggca agtggatgct acaggttgta acaagttact gtccagacgt
     2941 agctcacaaa taggaattca tcaagatctg gacatctgat gagcaatccc tacaatcgcc
     3001 gcgtacttta atttttcagg atacatcatg acccccgaac accttccaac agaacagtat
     3061 gaagcgcagt tagccgaaaa agtggtacgt ttgcaaagta tgatggcacc gttttctgac
     3121 ctggttccgg aagtgtttcg ctcgccggtc agtcattacc ggatgcgtgc ggagttccgc
     3181 atctggcacg atggcgatga cctgtatcac atcattttcg atcaacaaac caaaagccgc
     3241 atccgcgtgg atagcttccc cgctgccagt gaactcatca accagttgat gacggcgatg
     3301 attgcgggtg tgcgtaataa tcccgttctg cgccacaagt tgttccagat tgattacctc
     3361 accacgctga gtaatcaggc ggtggtttcc ctgttgtacc ataaaaagct ggatgatgag
     3421 tggcgtcagg aagcggaagc cctgcgcgat gcactgcgcg cgcagaatct gaatgtgcat
     3481 ctgattggtc gggcaacgaa aaccaaaatc gagctggatc aagattacat cgatgaacgt
     3541 ctgccggtcg cagggaaaga gatgatctac cgtcaggtag aaaacagttt tacccagccg
     3601 aacgcggcga tgaatattca gatgctggaa tgggcgctgg acgtaaccaa aggctcaaaa
     3661 ggcgatttac tggagctgta ctgcggcaac ggtaactttt cattagcact ggcgcgcaat
     3721 tttgatcggg tattagccac cgaaatcgct aagccgtcgg ttgctgctgc gcaatacaac
     3781 atcgcagcta accatattga taacgtacaa attattcgta tggcggcaga agaatttact
     3841 caggcgatga atggtgtacg cgagtttaat cgcctgcaag ggatagactt gaagagttat
     3901 cagtgcgaaa ccatttttgt cgacccaccg cgcagcggtc tggacagtga aaccgagaaa
     3961 atggtgcagg cgtatccacg tattctgtat atctcctgca atccggaaac gttatgcaag
     4021 aatctggaaa cattaagcca gacgcacaag gtcgaacgtc tggctctgtt tgatcagttc
     4081 ccctacacgc accatatgga gtgcggcgta ttactgaccg cgaagtaaaa cccatgccgg
     4141 atgcgccagc atccggcata ataccgatta ctcagcaact tcctgcttac ggttacgcat
     4201 cttcgcgcca atccagaaca ccataatgac ggagagtact gccgggaaga agttagagcc
     4261 gatatccgga tattccgcac gaactaccgt gctgtacagc agtacgccga gaataaaaca
     4321 ggcagctgcc agacccggca agcctaccgg catggtgcga ttaagataac gttgatgcag
     4381 gcagtaaacc gtcagcacca gggaaataat cgggaataca gaaaatggca caatggagct
     4441 aaacagcgct gcgaaagtac cattaatcga taagccagca accaacgcca gcagcagcgt
     4501 acctctatct tgatttgctt gtttcattac tcgtccttca catttcccgg aataattgcg
     4561 gttttctctt gttcacggcg ataccagtaa taagcccctt tcgaaatcat tcgcagttgc
     4621 agtaccagtc gctcttctaa ttgccgacgt tgttcgacgc cgacgtccaa cgcctcggca
     4681 cccgcactga agacaattgt caccattgct tcggcttgcg cttcagtaaa cgcacgcggc
     4741 atatggtttt cgagttccag atagtccgca agttccgcaa tgaagtgctg aatttcacgc
     4801 gcaacggcgg cacgaaacgc agcggaggtg ccggagcgtt cccgcaataa taaccggaag
     4861 gcgttaggat tattaccgat gaactccata aatgtggaga ccgaggtgcg gatcacactc
     4921 ccgcctttgg cgatacgctg acgcgcctgg cgcatgagtt ggcgtagcat taaaccgctc
     4981 tcatcaacca tggtcagacc cagttcgtct acgtcgcgga aatgccgata aaaagaggtg
     5041 ggagcaatgc ccgcttcacg cgccacttca cgcaaactca ggctggcgaa gctgcgttca
     5101 gcacttaatt ggctaaatgc ggcttccacc agcgaacggc gggttttttc tttttgttgc
     5161 gctcttacgc ccatcacgat gtctgaatcc ttgccaaaag tactgctggc actataccag
     5221 agaatgaaca tactggattg ctccagtatt gtgaatgaac ggtaacgcaa taaaacatat
     5281 ttactgcaat tctttggccg gttcttttac gtacagcgga aacctgccgc ttaaacggag
     5341 agtatcgtcg ataaaaatcc aataaaacgt cagggcaaaa gtaagaaaca gacaaagcaa
     5401 aggccgctca ggatatagcc agataaatga cggggatcaa ttggcttacc cgcgataaaa
     5461 tgttaccatt ctgttgcttt tatgt
//
LOCUS       DAMZET010000233         5449 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.233, whole genome shotgun sequence.
ACCESSION   DAMZET010000233 DAMZET010000000
VERSION     DAMZET010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.233"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..134)
                     /gene="sra"
                     /locus_tag="POB34_004411"
     CDS             complement(<1..134)
                     /gene="sra"
                     /locus_tag="POB34_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HDI5921249.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     "
     gene            complement(236..451)
                     /gene="bdm"
                     /locus_tag="POB34_004412"
     CDS             complement(236..451)
                     /gene="bdm"
                     /locus_tag="POB34_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HDI5921250.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAQHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            796..1227
                     /gene="osmC"
                     /locus_tag="POB34_004413"
     CDS             796..1227
                     /gene="osmC"
                     /locus_tag="POB34_004413"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HDI5921251.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(1283..2209)
                     /locus_tag="POB34_004414"
     CDS             complement(1283..2209)
                     /locus_tag="POB34_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5921252.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRV
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(2202..3188)
                     /locus_tag="POB34_004415"
     CDS             complement(2202..3188)
                     /locus_tag="POB34_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5921253.1"
                     /translation="MTQPVLDIQQLHLSFPGFHGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIDLREEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            complement(3185..4081)
                     /locus_tag="POB34_004416"
     CDS             complement(3185..4081)
                     /locus_tag="POB34_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HDI5921254.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(4078..5100)
                     /locus_tag="POB34_004417"
     CDS             complement(4078..5100)
                     /locus_tag="POB34_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5921255.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQHLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFSATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLSGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            complement(5102..>5449)
                     /locus_tag="POB34_004418"
     CDS             complement(5102..>5449)
                     /locus_tag="POB34_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921256.1"
                     /translation="GKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENS
                     EVDKLLRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNP
                     MLEQVFNINTMSK"
BASE COUNT         1389 a         1449 c         1370 g         1241 t
ORIGIN      
        1 ttttcagcgg ggcgttttct gccggttggg ttatttacca cgctggattt gtcaccttcg
       61 gtaactattt tgcgctggtt agaaatttta tggtccagtc caagaatatg acgtgcctga
      121 cggttcgatt tcattttaac tcctcaatcc tgttgctagt tttaaggaca acatcgccgt
      181 agcgaagaaa cacgtgctaa acccctaaat taggttgccg atcaagcata gcaccttaaa
      241 gcgtagggtg ctggccactg accacataat tgatcgtttg ctggtagata tcactgagga
      301 tgtcgttatc agaagcttca acccatttgg tcagctccat gagaatgtca tcttcagtga
      361 caacaccgtg ctgtgcccga aggccttgct caatggcatt aaccagagcg ggttctgctg
      421 ttgaattttc tgcctgataa taagtaaaca tagtgattct ccgtgtctgt gtatttatgg
      481 tgtctgctac ggatcgcaga tttataaagc acattcagca tggcaaatat ttgccgcttc
      541 gttgttaaga ttagtcctgg ttgatgattt ttatatttta acaccatgat attcataggg
      601 attgtgattg gtatgatccg attaatattg atacaatatc ttttgggtta tatattcccg
      661 gtaatctatt gtgggaattt aatttaagtg cagaagtaat attttcgccg gattttattc
      721 ggaatatcct gcttatcctc gtgctgtttc tcacgtagtc tataatttcc tttttaagcc
      781 cacaggagag caacaatgac aatccataag aaaggtcagg cacactggga aggcgatatc
      841 aaacgcggga agggaacagt atccaccgag agtggcgtgc tgaaccaaca gccgtatgga
      901 tttaacacgc gttttgaagg cgaaaaagga accaaccctg aagaactgat tggcgcagcg
      961 catgccgcat gtttctcaat ggcgctttca ttaatgctgg gggaagcggg attcacgcca
     1021 acatcgattg ataccaccgc cgatgtgtcg ctggataaag tggatgccgg ttttgcgatt
     1081 acgaaaatcg cactgaagag tgaagttgcg gtgccgggta ttgatgcctc tacctttgac
     1141 ggcatcatcc agaaagcaaa agcaggatgc ccggtctctc aggtactgaa agcggaaatt
     1201 acgctggatt accagttgaa atcgtaaagc attgccggat gacgcgtcag gcgcgtgaat
     1261 gcctgatgcg ttgttagcat ctctacagcg cccgccagca ccggagctca cgtccgtcct
     1321 cccttattgc taatgattgc cggacttcac atccgtgggt tgccagcggg caacgttcgt
     1381 agaaaaaaca gccttgcggc aacgtgcggt ttcccggcag atccgtttta cgtaatgccc
     1441 attcttcctc cagcggttta tcaatggcgg ggagggaatc cagcaataat cgggtgtatg
     1501 gatgtgcagg tgccgtcagc acctgctgcg cgtcgcccag ttctacaatc tgcccgagat
     1561 acatcaccgc cacccgatcg ctcatatgac gtatcaccga gacattgtgt gaaatcagca
     1621 cataggtcag cccgtgattt tcctgtagcg ttaccagtaa attgaggatc tgcgcctgaa
     1681 cggagatatc cagcgcagag gttggctcat caagcacaat cacgtcaggc tgcgaagaga
     1741 gtgctctggc aatggcgata cgttgccgct gcccgccgga gaacgcatga ggcaggcggt
     1801 cgagatactc cggacgaata cccacctgca cagccaactc ctctgccaac gctcgccgct
     1861 gttgttcact actacgctta gctatccaga gcggttctgt gatgacgcgc cacaccggta
     1921 agcgcggatt aagcgaagag agcgggtcct gaaacaccat ctgcataatg cgttgtgagc
     1981 cagaacggat gtactgcccg tggctcggtt gcagcatacc cattaaaagc tgtgcgaggg
     2041 tgcttttgcc gcagcctgac tcgccgacga tccctaaggt ttcaccacgg cggatctgta
     2101 aatcaatacc attaatggca tgaacatgtt ccgtagtttt accaagccag tttttacggg
     2161 ccgggaaatt gatatggacg tcgcgtaacg ttaataacgt gtcagacact tatgacctcc
     2221 tgctgcggat accaacaggc gcagcgctgg ttgttgtcac cacacgctgt cagcgccggg
     2281 acgttttcac actgtgcacc tgccgcatag caacgatcgc gaaaagcgca gccgtcaggc
     2341 aaatgggtga ggtttggtac cgtccctgga atggcgggta atagctggcg tggtactcca
     2401 tgttctggtg cgcattgcag caaaccaatg gtatacggat gccggggatg atggataacg
     2461 tctgccgtca cgccgctttc aattacgctt ccggcataca tcacgtaaac gctatcgcac
     2521 agttgcgaca ccacggccat atcatggctg atgaacagaa ccgcagttcc actggcgcgg
     2581 gctttatgtt taagcaaacg cagtacctgc aactgcaccg tgacgtccag cgccgtagtc
     2641 ggttcgtcgg caataatcaa ttgcggctcg caggagaatg ccagcgcaat cattacccgc
     2701 tggcgcatac cacctgaaag ctcaaacgga tagcgcgaca taacttccac ggcatccggg
     2761 atttgcatct cttcccgcag gtcaatcgct ttagctctgg cttcccgacg acttattggt
     2821 tgatgatggc ggatcacgtc catcatctga agacctattc gacgtgtcgg attgagggcg
     2881 gtcatcggtt cctgaaagat catcgccact cgtgcgccgc gccactgacg aagctgtttt
     2941 tcccgcgcgt taagaacatc ttctcccagc agggaaatct gcccccgatg tacgcaataa
     3001 ctgcccgtcg gtagcagacg cataatcagc attgcggtga ctgatttacc tgagccggat
     3061 tctcccacca gaccgacaat ttcaccgcgg ttaatctgca aggacacatt gttgagcgcg
     3121 tgaacatcac cgtgaaaacc ggggaaactc aaatgcagtt gttgaatgtc cagaacgggt
     3181 tgggtcatga ctgctttcct cctgctttcg ggtcaagcag atcgcgaata ccatcaccaa
     3241 agagattaaa cccgacggcg gtaagcaaaa tcgctgcccc cggaaatgcg caataccacc
     3301 actgatcgag tacatagttg cgaccatttg ctaccatcgc gccccattca gcactcggtt
     3361 gttgagcacc caggccaata aatcccaacg tggcggccat taaaatcgcg ctaccgatat
     3421 ccagcgatgc ctgcacgatc agcggcggta gggagttacg taaaatatgc cagttgatca
     3481 gatgccaacg agacgcgcca aaggttttcg ccgcctgaac ataggtatac tggcgtacaa
     3541 ctaatgcttg cccccgcgcc aggcgcacat aaaagggaat tcgcacaata gcaatagcca
     3601 gcatggcgtt aaacaaactc ggcccgagag cggcagccag tgccattgtc agtaccaacg
     3661 aaggaatcga cagcataatg tccatgatgc gcatgatgat ggcgtctgcg cgtccgccaa
     3721 gcacaccgga tagacatccg agtagcgaac caatcatacc cgcaatggcg accaccacta
     3781 atccggcgag aattgattgc tgactgccga ccagtacgcg gctgaacaga tcgcgtccca
     3841 cttcatcggt gccaaaccag tgcgccgcag aaggcggcaa aaggcgggcg gttaaatcaa
     3901 tggcgttggg atcatgcgtc gccagccacg gtgaaaaaat catcatcaga agcattaata
     3961 caatgatgac tgcgccggtc acggtgagcg gactgccttt cagcatccat accagttttg
     4021 caccattaaa tcgcgtttgt ttttgtggac gtacggcgga cgtttcctcg cttagcatca
     4081 ttcaccacct ccacgtccga tacgcggatc aatccacaaa tagagcaaat ccaccaccag
     4141 gttgaccagc acataagcaa atgaaaccac gacggcaaag cccatcacag ccgggaagtc
     4201 gagcgcctgt attgatgtca ctacccatgc acccattccg ggccaggcaa aaacggtttc
     4261 ggtgagcact gcgccataca acaaatcgcc cagcgccaga cccaatacgg taatcgatgg
     4321 gatcaacgca ttgggtagcg cataacataa aacgatatac cagccggaca agccgctggc
     4381 cctggcggta cgaatgtagt cttcactcaa ttgttccagc atcgctgaac ggatctggcg
     4441 ggcgacaatc cccaggtgaa caaacgccag cgttaatgcc ggtaagatga gatgttgcaa
     4501 cgcattgaag aagacttcac cgttgccttc aagcagcgca tcgagcagat aaaagccggt
     4561 aacgtgcgtt ggcggatcca gccagtcatc aagccttccg ccgccgggaa gaatttgcag
     4621 atgaccataa aacagcacaa tgacgcccag cccaagccag aacgcaggtg tggagattcc
     4681 ggtaatggcc attattcgca ccagatgatc cagccagcga tttcgccaga cggcagagag
     4741 tatgcccagc ggaatgccaa tcaggagtgc cagcagcagg gcacaaaaag ccagttccag
     4801 cgtcgccgag aaaaatatac gcaactcttc cagcaccgga cgcccggtac gaatggatgt
     4861 tccgaggtca ccatgaaaca ggtcgctgac gtagcggtaa aactgtacgt acagtggctg
     4921 gtccagtccc agatgctggc gaatattttc cacgatagca tcgctggcgc ggtcacccgc
     4981 cagtaaccgc gccggatcac cagggatcag atgcgagata ataaacgtaa tcacgcagac
     5041 gcccgccacc acgagcacca gcccccagca gcgttggcgt aaaatactcc agaaggtcac
     5101 gttatttact catggtattg atattgaaga cctgttccag catgggattg aacacaaagc
     5161 ctttcacctc tttgttcatc gccagttggt agtttttctg gaacaggtac acataagcag
     5221 cgtcatcaat gacgattttc tgtgcctgct ggtagtcccg ggtacgctgc gtctggtcgg
     5281 tggtcgcaag cgcattgcgc agtaacttat cgacctcact gttttcatag aacgagcggt
     5341 tacccggcag accttttttg tctgactcaa accagtaatt cataaacata tacgggtcgg
     5401 caaaatccgg actccagttg ccaatcgcaa tgtcgtaatc acctttacc
//
LOCUS       DAMZET010000234         5345 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.234, whole genome shotgun sequence.
ACCESSION   DAMZET010000234 DAMZET010000000
VERSION     DAMZET010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.234"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            270..458
                     /locus_tag="POB34_004419"
     CDS             270..458
                     /locus_tag="POB34_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HDI5921257.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPIQKGCWQ"
     gene            455..646
                     /locus_tag="POB34_004420"
     CDS             455..646
                     /locus_tag="POB34_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HDI5921258.1"
                     /translation="MNTAFALVLTVFLVSGEPVDIAVSVHRTMQECVTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            739..3210
                     /locus_tag="POB34_004421"
     CDS             739..3210
                     /locus_tag="POB34_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HDI5921259.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEASASFVPVETESDPM
                     NVTFDNLAPEVQNAVMVKFHTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKEAGKYTSVVDLARA
                     RTNQQHTENSTGKINPVIAAIHREYKQTWKILDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGTNQSATDTLSSPVPETDAVETAIPDNEKTECKVEVEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLDKALSAGATEITKEEYFARKNGTYSGSQQNTGASDTTAQ
                     PGSVKVTADEVNKIMQAANISQPDADELLAVSRGEFVEGISDPNDPKWVKGIQTRDSV
                     NQNQQETEQNDQKAEQNSPNTQQNEPETKQPEPVVQQEPEKICTACGHSGGGNCPDCG
                     AVMGDATYQEIFDGENQPEVQENDPEEMEGTAHQHKENTGGNQHHASDSETGEASDPL
                     IKANGHHNLTSTSRAGIHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVHVWG
                     NGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSAIWQKLIPSQADS"
     gene            3283..3534
                     /locus_tag="POB34_004422"
     CDS             3283..3534
                     /locus_tag="POB34_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HDI5921260.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            3569..4849
                     /locus_tag="POB34_004423"
     CDS             3569..4849
                     /locus_tag="POB34_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HDI5921261.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRRVAGEL
                     RASVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKHKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSEFAPTMPHNEAMKN"
     gene            complement(4869..4979)
                     /locus_tag="POB34_004424"
     CDS             complement(4869..4979)
                     /locus_tag="POB34_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HDI5921262.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFKSHEC"
     gene            complement(5037..>5345)
                     /locus_tag="POB34_004425"
     CDS             complement(5037..>5345)
                     /locus_tag="POB34_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HDI5921263.1"
                     /translation="HPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKELSIFS
                     SRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVLLTFSE
                     "
BASE COUNT         1649 a         1180 c         1252 g         1264 t
ORIGIN      
        1 cgcttaagtg acaaccccgc tgcaacgccc tctgttatca attttctggt gacgtttggc
       61 ggtatcagtt ttactccgtg actgctctgc cgcccttttt aaagtgaatt ttgtgatgcg
      121 gtgaatgcgg ctgagcgcac gcggaacagt taaaaccaaa aacagtgtta tgggtggatt
      181 ctctgtatcc ggcgttaatt gttaactggt taacgtcacc tggaagcacc aggcaccgca
      241 tcacaaaatt cattgttgag gacgcgataa tggaaacgtt attaccaaac gttaatacgt
      301 ctgaaggttg ttttgaaatt ggtgtcacta tcagtaaccc tgtatttact gaagatgcca
      361 ttaacaagag aaaacacgaa cgggagctat taaataaaat atgcattctt tcaatgttgg
      421 cccgtttacg tccgatacaa aaaggatgct ggcaatgaat acagcatttg cacttgttct
      481 gacagttttt cttgtttccg gagagccagt tgatattgca gtcagtgttc acaggacaat
      541 gcaggagtgt gtgactgcag caaccgaaca gaaaattccc ggtaactgtt acccggtcga
      601 taaagttatt caccaggata ataacgaaat cccggcaggt ctttaaaaca gttccgtaat
      661 aaacatccga tttcattctt atatgccagc aatggcaggg atttgttcac ccttaaatct
      721 gtaatgaggt aaaacaaaat gagtaaagtc tttatttgcg ccgccattcc ggacgaacag
      781 gcaataaagg aagaaggtgc cgtcgctgta gccactgcca ttgaagccgg tgatgaacgt
      841 cgcgcccgcg caaaatttca ctggcaattc ctggaacatt atccggctgc tcaggactgc
      901 gcttataaat ttcttgtttg cgaggataaa cccggtatac cccgccctgc cctcgattcc
      961 tgggatgctg aatatatgca ggaaaaccgc tgggatgagg cgtctgcttc ctttgtcccg
     1021 gtcgagactg aatccgatcc gatgaacgtc acttttgaca acctagcccc tgaagtacag
     1081 aacgctgtca tggttaagtt ccacacatgt gaaaacatca ctgttgatat ggtgattagc
     1141 gcacaggaat tgttgcagga agacatggca acattcgacg gccatatcgt tgaagcgttg
     1201 atgaaaatgc cagaagttaa cgctatgtat ccggagctta agctgcatgc catcgggtgg
     1261 gttaagcata aatgtaagcc tggtgccaaa tggcccgaaa ttcaggcaga gatgcgcatc
     1321 tggaaaaaac gtcgcgaagg tgaacgcaag gaagccggaa aatacacgtc tgttgttgat
     1381 ctcgcccgcg ccagaaccaa tcaacagcac actgaaaatt caacaggaaa aatcaacccg
     1441 gtcattgctg ccattcatcg cgaatacaag cagacatgga aaatactgga tgacgaactg
     1501 gcctacgctc tctggcctgg tgatgtggat gccggaaaca ttgacggcag catccatcgc
     1561 tgggcaaaaa atgaagttat cgacaacgac cgcgaagact ggaagcgtat ctcggcatca
     1621 atgcgcaaac agcctgatgc ccttcgctac gaccgccaga ctatttttgg ccttgtccgt
     1681 gaacgtccga tcgacattca caaagaccct gtggcactga acaaatacat tactgaatac
     1741 ctgactacaa agggcgtgtt tgaagatgaa ggaacaaatc agagcgcaac tgatactctc
     1801 tcgtcgccag taccagaaac tgatgcagtg gaaacggcaa ttccggacaa cgaaaaaacc
     1861 gaatgcaaag tggaagtcga accatctgta gaacgtgagg ggccgttcta cttcctcttc
     1921 accgacaagg atggcgaaaa atacggtcgc gcaaacaaac tttctggtct ggataaggcg
     1981 ctgtctgctg gggctactga aatcacgaaa gaagaatatt tcgcccgcaa aaacggtaca
     2041 tactcaggtt cacaacaaaa tactggtgca tctgacacga ccgcacaacc agggtcagta
     2101 aaagttaccg ctgacgaagt aaacaaaatt atgcaggcag ccaatatcag ccagcctgac
     2161 gccgatgaac tgcttgcagt atcacgtggt gaatttgttg aagggattag cgacccgaat
     2221 gatccgaaat gggttaaggg gatccagacc cgcgattctg tgaaccagaa ccagcaagaa
     2281 acggaacaga acgaccagaa agcggaacaa aacagcccaa atacgcaaca aaacgagcca
     2341 gaaacgaaac aacctgaacc agtagtgcaa caggaaccgg aaaagatctg caccgcctgc
     2401 ggtcatagcg gtggcggcaa ctgccctgat tgtggtgcgg tgatgggtga cgcaacatac
     2461 caggaaatat tcgatggaga gaatcagcct gaagttcagg aaaatgatcc ggaggaaatg
     2521 gaaggcactg cgcatcagca caaggagaac actggcggca atcagcatca tgccagcgat
     2581 agtgaaactg gcgaggcgtc agatccctta attaaggcga acggtcatca taatctcaca
     2641 tccaccagca gagcggggat tcatctgatg atcgaccttg aaaccatggg aaaaaatccc
     2701 gatgccccga ttatctcaat aggtgcaata tttttcgatc cgcaaaccgg agatatggga
     2761 ccggaattta gtaagactat cgatctggaa actgctggcg gagtcattga tcgggacacc
     2821 attaaatggt ggcttaaaca atcacgcgaa gcgcaatctg ccattatgac cgatgaaatc
     2881 ccgttagatg atgcactgtt acaattgcgg gaatttatcg acgaaaactc cggtgaattt
     2941 tttgttcatg tctggggaaa tggagccaac ttcgacaaca cgattttgcg ccgttcatac
     3001 gaacggcagg ggatcccctg cccgtggcgt tactacaacg atcgcgatgt acgcacaatc
     3061 gttgagctgg ggaaagccat agacttcgat gccagaacgg ctattccatt cgaaggtgag
     3121 cgccataatg cacttgatga cgcccgttac caggcaaaat acgtttcagc tatctggcaa
     3181 aaactgatcc cgagtcaggc tgattcttaa tgttcaactg tcgccggttg tgactggtat
     3241 tctgcaaccg gcgctcgtct gatgtaagag ataaagaaat cgatgagcga agtaatcatg
     3301 attgtctctc ccggcaaatg ggtatccgaa gagcagttaa ttgcgctgaa aggaataaaa
     3361 aaaggtacgt taaaaaaggc ccgggaaaaa tcgtttatgg aaggaaggga atataagcat
     3421 gtcgctcatg acggtatgcc atgggataac agtccatgct tttacaacct ggaagaaatt
     3481 gatcgctgga ttgagcgcca ggcatcagca agaccaagac gtcatcttac ttgactaaaa
     3541 gccacactaa ctaatgagag aagttgaaat gaaatatccg acaggcgtgg aaaaccatgg
     3601 agggaaatta cgtatctggt ttgtttataa aggcgtaaga gtcagggaaa acctgggggt
     3661 tcctgacaca gcaaaaaaca ggcgcgttgc aggtgagcta cgcgcctctg tttgttacgc
     3721 aataaaaact ggtgttttcg actatgcaaa acagtttccc tcctcacgca atctggaaaa
     3781 atttggtgag gcccgacaag atttaaccat aaaagaactg gctgaaaaat ttctggcact
     3841 gaaagaaact gaagtcgcaa aaacatcact caacacatac cgtgccgtca tcaaaaatat
     3901 cctgagcata atcggtgaaa aaaatcttgc ctcatcgatt aataaagaaa aattactgga
     3961 ggttcgtaaa gagctactga ctggatacca gatccccaaa agtaactata ttgttacaca
     4021 accagggaga tcggctgtaa ctgtaaataa ttacatgaca aatcttaacg ccgtgttcca
     4081 gtttggtgtt gataacggtt acctggcaga taacccgttt aaggggatct cgccattaaa
     4141 ggaatcaaga accattccgg atcctctttc gcgggaggaa ttcatccgtc ttatcgatgc
     4201 gtgcagaaat cagcaagcca aaaatttatg gtgtgtttct gtttatactg gcgttcgccc
     4261 tggtgagctg tgtgcacttg gatgggagga catagatctg aaaaatggaa caatgatgat
     4321 caggagaaat ttagcaaaag accgtttcac ggtaccaaaa acacaggcgg gaaccaatcg
     4381 ggtcattcat cttattaagc cagcaatcga cgctctccgg agtcagatga cattaacgag
     4441 actgagcaaa gagcatatca ttgatgttca cctcagagag tatggcagaa cagaaaaaca
     4501 taaatgcacc tttgtttttc aacctgaagt gtcagcgaga gtaaaaaatt atggtgacca
     4561 ttttaccgtt gactcaataa ggcagatgtg ggacgcagcg ataaaacgtg ccggactccg
     4621 ccatcgaaaa tcatatcagt cgagacatac ttatgcctgc tggtcgctga cagctggtgc
     4681 taacccagca tttatagcaa accagatggg ccatgcagat gcgcaaatgg tatttcaggt
     4741 atacggaaaa tggatgtctg aaaacaataa tgcacaggta gctttgttaa atacacagtt
     4801 aagcgagttt gccccaacca tgccccataa cgaagcaatg aaaaattaat ttaatattta
     4861 tcaaatagtt aacactcatg actcttgaaa tccataaatt caagcgcagt gcccagccat
     4921 cccgatactg ctgctttcac caaatcctta gtgcttcttt catgtttttc tattgtcata
     4981 atggttatct ctaaaaaaga ggtaagatgc gtactactta ctcgccgtta ttggtattat
     5041 tcagaaaaag tgagtaagac tttgcagcaa tgtttttgat cctgttcaaa taaactaatg
     5101 gcatcagcaa catgctggaa atcaaacgta tgggtaatta atttttctgg tttaattaac
     5161 cctttactta accagtcgat aacaaccgga aatttatttg catttaagcg tgaagagaaa
     5221 atagagagtt cttttccggt aattccttgc tgaatcactt cagacggttc actggagaag
     5281 cccatcaata caatacgtgc cgctggagaa gccagcgtta cggcctcttt caggatagaa
     5341 ggatg
//
LOCUS       DAMZET010000235         5323 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.235, whole genome shotgun sequence.
ACCESSION   DAMZET010000235 DAMZET010000000
VERSION     DAMZET010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.235"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..447)
                     /locus_tag="POB34_004426"
     CDS             complement(<1..447)
                     /locus_tag="POB34_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HDI5921264.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQA"
     gene            633..1085
                     /gene="matP"
                     /locus_tag="POB34_004427"
     CDS             633..1085
                     /gene="matP"
                     /locus_tag="POB34_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HDI5921265.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(1161..2213)
                     /gene="ompA"
                     /locus_tag="POB34_004428"
     CDS             complement(1161..2213)
                     /gene="ompA"
                     /locus_tag="POB34_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HDI5921266.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQG
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(2570..3079)
                     /gene="sulA"
                     /locus_tag="POB34_004429"
     CDS             complement(2570..3079)
                     /gene="sulA"
                     /locus_tag="POB34_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HDI5921267.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            3298..3927
                     /gene="sxy"
                     /locus_tag="POB34_004430"
     CDS             3298..3927
                     /gene="sxy"
                     /locus_tag="POB34_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HDI5921268.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(3890..>5323)
                     /gene="yccS"
                     /locus_tag="POB34_004431"
     CDS             complement(3890..>5323)
                     /gene="yccS"
                     /locus_tag="POB34_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HDI5921269.1"
                     /translation="YFVAQDIHERASSSHIQYQTLREHFRHSDVLFRFQRLMSMQGQA
                     CQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTLGFLLNNLRAID
                     AQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESALFRHAVRMSLV
                     LCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLVGIAIGIPVLWF
                     VPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEGFEVALPRVIDT
                     LIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQGRDNRLAYRIA
                     RRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYISALGAHREQLTN
                     PEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRADSKEPLVVQQVG
                     LLIALLPEIGRLQRQITQVPQETPVSA"
BASE COUNT         1427 a         1272 c         1341 g         1283 t
ORIGIN      
        1 tgcctgatgc accagaccag gctgcagggt aatgtcgcca ttaaactggc gcaggcagcc
       61 aaagagttgc tccgcttcta cccagtcggc agcgacaact tgcgttaaag tcgcaaaatt
      121 atcatctgca ctcactgcgt gacgtaagcg gatggagtgg ccggaaacct catagtgacc
      181 gcccaccagt tggcctgcat cgctttgtag cgtacgcgcg gcattggcaa taagattcag
      241 atactcagac tcttccgggg ccttcgccag cataaaagag gaggatgctc gcgtatgcag
      301 caactgctcc agcgcaaatt gcagccgcgg ttgagtatca ctgaataaag gatcgttttc
      361 gtcaatcaaa tgtggctgag caaatatttc ctgatagcta tcggtatcag gaaccaggtc
      421 acgccatgca agtttcgtaa tggtcaaagt tgatgttttt tagtctgttg tcaaagccgc
      481 gattataccg taaccggcac tacagcacac gtagaaagca ccgacaatac tcctggcatg
      541 ggcgttaaag ctcacaggat ggagattctt ttcttcactg gcctaaaaag ctgatattct
      601 gtaaagagtt acacggtaac attgagatcg ctatgaaata tcaacaactt gaaaatcttg
      661 aaagcggttg gaaatggaag tatctggtga agaagcatcg cgaaggggag ttaatcaccc
      721 gttacataga agccagtgcc gcccaggaag ccgtcgatgt gttgctctcg ctggaaaatg
      781 aacccgtgct ggtaaatggc tggattgaca agcatatgaa tccggaactg gtcaatcgca
      841 tgaagcaaac cattcgggcc agacggaagc gccattttaa tgcggaacac cagcatacgc
      901 gcaaaaaatc gatcgatctg gaatttattg tctggcaacg tctggctggt cttgcgcagc
      961 gtcgcggtaa aacgctttct gaaacgattg ttcagctgat tgaagatgcg gaaaacaaag
     1021 agaaatacgc gaataaaatg tcttctctga agcaggatct gcaggcattg ctgggtaagg
     1081 aataactgac gaaagtcagt tcaatttact aaaggcaaaa aaaaccccgc agcagcgggg
     1141 tttttctacc agacgagaac ttaagcttgc ggctgagtta caacgtcttt gataccttta
     1201 acttcgatct ctacgcgacg atccggagcc aggcagtcga tcagtgcagc acgctgtttc
     1261 acgttgtcac aggtgttgcc agtaaccggg ttggattcgc ccataccacg tgcggagatc
     1321 ttgtctgccg ggataccttt ggagatcagg taatcaacaa cagactgagc acggcgctcg
     1381 gacagaccct ggttgtaagc gtcagaaccg atgcggtcgg tgtaacccag aacaactacg
     1441 gaaccgtctt tcggatccag gttgctcagc tggctgtaca gctgatccag agcagcctga
     1501 ccttccggtt tcagggttgc tttgttgaag ttgaacagaa cgtcagactt cagagtgaag
     1561 tgcttggtct gtacttccgg tgccggagct ggagccggag caactactgg agctgcttcg
     1621 ccctgaccga aacggtagga aacacccagg ctcagcatgc cgttgtccgg acgagtgccg
     1681 atggtgtgtg cgtcaccgat gttgttggtc cactggtatt ccagacgggt agcgatttca
     1741 ggagtgatcg cgtactcaac accacctgcg aaaaccggag aaacgccagt gtcgtggtct
     1801 ttaaaggatg cgccaccagg tacgttagcc ttggtgtctg cacgccatac cataccaccc
     1861 agacgagtgt atacgtccag atcgtcagtg attgggtaac ccagtttagc agtcagttga
     1921 acgccctgag ctttgtatgc accgttgatg ttgtcgcctt tgtacggcat acgacctaac
     1981 cagtcgtaac ccatttcaaa accaacatac gggttaacct ggtaaccacc aaaagcacct
     2041 gcacccagtt ggttttcgtg ggtcggacca ttgttaggaa taaaaccagt gtcatggtac
     2101 tgggaccagc ccagtttagc accagtgtac caggtgttat ctttcggagc ggcctgcgct
     2161 acggtagcga aaccagccag tgccactgca atcgcgatag ctgtcttttt cattttttgc
     2221 gcctcgttat catccaaaat acgccatgaa tatctccaac gagataacac ggttaaatcc
     2281 ttcaccgggg gatctgctca atattaactc taccgatatc ttcggcttat gccgagcacc
     2341 cctggcgatg taaagtctac aacgtagttg aaaacttaca agtgtgaact ccgtcaggca
     2401 tatgaaaaaa aagtcttgta taaggtatgt ttaatctttt ttgtcagcga caatttacag
     2461 aagaaaatcg cggaaaccgc ttcagacaag cctccgcaag gaaaattagt cacgactgaa
     2521 agcattggct gggcgacaaa aaaagttcca ggattaatcc taaatttact taatgataca
     2581 aattagagtg aatttttagc ccggaaagtt gtctcgtggc gtgagaggat gcgcttaccg
     2641 gacgcataat aaaccccata gcgttacctt catttgccgc atcaacaagt tcagcatgct
     2701 cttcttcagt caaatcatct gccaaccaac cgatcaccac actgtaattg cccgtgcgta
     2761 aagcgcgaac cattgactcc acagtgtggc aaggggagag ctggctaatc tgcattactt
     2821 tcgttaaggg tagcccagat gcctgaaccc attcccgact cagtttttgt tgcggtgtta
     2881 accagagttg ccagcgcgat tgctgaccga gttgctgtaa caatggcaac agtagaagtt
     2941 gcgtcatcat gggctgatct tcgcgataga caacttcact gataagcccg gctgtagtgt
     3001 tttccgtaga gacacgcgca attttacttg ctgcggatga gaacgacgaa gaacgatgtg
     3061 catagcctga agtgtacata atcaatccag cccctgtgag ttactgtatg gatgtacagt
     3121 acatccagtg acaacaaaga tcaaccctat tttcggaaag agcctcgcaa attttgtcgt
     3181 tggtgacggg aaaacataaa ttaatcttgc cccttaagaa taagttgcct attttcgtag
     3241 ttaacggatc cgttaatgtg aatcattctt ttatgttatg attttaaaag gaattttatg
     3301 aaaagcctct cctataagcg gatctataaa tcacaagaat acctggcaac gttgggcaca
     3361 attgaatacc gatcattgtt tggcagttac agcctgaccg ttgacgacac ggtgtttgcg
     3421 atggtttctg atggtgagtt gtatcttcgg gcttgtgagc aaagtgcaca gtactgtgta
     3481 aaacatccgc ctgtctggct gacatataaa aagtgtggcc gatccgttac cctcaattac
     3541 tatcgggttg atgaaagtct atggcgaaat caactgaagc tggtgcgtct gtcgaaatat
     3601 tctctcgatg cagcgctgaa agagaaaagc acgcgcaata cccgggaaag actgaaagat
     3661 ttgcccaata tgtcttttca tctggaagcg attctcgggg aggtggggat taaggatgta
     3721 cgggcgttac gtatacttgg ggcaaaaatg tgttggttgc gactgcggca gcaaaacagt
     3781 ctggtgacag aaaagattct gtttatgctt gaaggtgcca ttatcggcat tcatgaagct
     3841 gcgctcccgg tggcacgccg ccaggagctt gcagaatggg ctgactctct tacgccgaaa
     3901 caggagtttc ctgcggaact tgagtaatct ggcgttgcag acgaccaatc tcaggcagta
     3961 atgcaatcaa taatccaact tgttgtacga ccagaggttc tttgctgtct gcccgtggtt
     4021 caagttgctg catccgctgt ttcaggctag ctaatgcctc attgacgcgt tcttcatcag
     4081 caggttgatg atgtaacgcg tcatcaacat agcaaactgc gtcatcaaga aacgccagaa
     4141 tttcaggatt agttaactgc tcccggtgag caccgagggc tgagatatag ctggtaaacg
     4201 tatggttaag gcacagcaac cgaaacgcgg cttcgcgaat ttgcggggta acgttcggct
     4261 cgctggacat atttgatacc accgacgcca gctcagcatc acggttgtgt gcatcgcggc
     4321 gggcaatacg atacgccaga cggttatcac gcccctgatg gtattgctcc agtatggcat
     4381 cgagataccg acagttggcc tctgtggcgc gttcgagcat gcgcggcaga ttgcgaaact
     4441 gccagtcagg ccagatgtag ctcactgccg cccacgcaat ggcacaacca atcagcgtat
     4501 cgattacgcg aggtaacgct acttcaaaac cttcacccag taagttaaaa cacagtagca
     4561 ccaaaagtgt gatgaacatc gttgcatgag cgtattgcac gttacggaag gcaaaaaaga
     4621 gcacgccggt aataaccagc agcaccagct gcccttccag tgatggcaca aaccacagca
     4681 caggaatgcc aatggcgata cctaccagcg taccaataat ccttaacttc aggcggtggc
     4741 gcgtggcgtt atagtttggc tggcagacaa acaaacttgt cagcaagatc caatacccgt
     4801 gatgcattcc ggttatttga atgatggcgt agccgaagca caacaccagc gacattctta
     4861 ccgcatgacg gaagagggcg gattccggcg tgaagtgacg gctaagacgc agccagatat
     4921 cactcaaccc gtgcgggctg tcatcagcga gctcattttc atcattatta tggggtagtg
     4981 cctgggcctg ttctgattca attgttgcca gttgggcatc aatggcgcgt aaattgttca
     5041 gcaaaaatcc cagtgttttg agtaaatcgg cgggtgcgcc gttatcgcgc atccgctcca
     5101 gcgcagcatc aatatgcgta aaagcgcgct caaaatgcgg atcatgttga taaggctgac
     5161 gcaacaaaat acagcgtgac agttgctggc acgcctggcc ctgcatcgac atcagccgct
     5221 gaaaacggaa cagcacgtca ctgtggcgaa aatgttcacg caatgtttga tactgaatat
     5281 gagaagagct ggcacgctcg tgaatatcct gtgcgacaaa gta
//
LOCUS       DAMZET010000236         5290 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.236, whole genome shotgun sequence.
ACCESSION   DAMZET010000236 DAMZET010000000
VERSION     DAMZET010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.236"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(213..1064)
                     /gene="hchA"
                     /locus_tag="POB34_004432"
     CDS             complement(213..1064)
                     /gene="hchA"
                     /locus_tag="POB34_004432"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HDI5921270.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPATFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDIAGRVYKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(1656..2714)
                     /locus_tag="POB34_004433"
     CDS             complement(1656..2714)
                     /locus_tag="POB34_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDI5921271.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKLDLYGKMVGERIL
                     TDRENGEKGDNSQDTSYARVGVKGETQINPELTGYGQFELDLEASNRHNPDQTRLAYA
                     GLSYKDFGSFDYGRNVGVAYDAEAFTDMFVEWGGDSWAGTDLFMTNRTNGVATYRNTD
                     FFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSVTYTIDGFSFAGAYANSDRIDFQS
                     SDSKGEKAEVWALSTKYDANNVYAAVMYGESHNMNSDDGDVVNKTQNFEAVLQYQFDF
                     GLRPSIGYSYSKALDVAGYKDSDRLNYIEIGTWYYFNKNMNVYTAYQINLLDKSDYVL
                     AHGLNTDDQLAFGIVYQF"
     gene            3281..3646
                     /locus_tag="POB34_004434"
     CDS             3281..3646
                     /locus_tag="POB34_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDI5921272.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
     gene            3686..4381
                     /locus_tag="POB34_004435"
     CDS             3686..4381
                     /locus_tag="POB34_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDI5921273.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSSAG"
     gene            4448..>5290
                     /gene="dcm"
                     /locus_tag="POB34_004436"
     CDS             4448..>5290
                     /gene="dcm"
                     /locus_tag="POB34_004436"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HDI5921274.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVL"
BASE COUNT         1442 a         1153 c         1283 g         1412 t
ORIGIN      
        1 ctttctttag tcaaagcgat tgccgaatta catgggggaa gtgctacgta tcactatctc
       61 aataagcata atgtgttccg gattatgtta ccgcaaagaa attaacatag ggcttcagta
      121 cgccataaat caaccatgaa aaaacgcagc cacgtcgtga gtattaacgt ggctgcgatt
      181 gattatgcgc ttacattcat acgtaacagg gattaacccg cataagctgc cagcatttcc
      241 tgcgccgcca gtttacccaa cgcattcgct gcgaaaggac tgtcgccggt gagaacttta
      301 cggtccttat atactcgccc ggcgatgtcg tcattaataa tgttcatgcc cattttcttc
      361 agctcttcgc cgaagtacca ggtgagatga cccggcatat agcctatctc tggcgtttgt
      421 ttgtctgcgg cgtctgggaa tgcgcaaatg gaataaccat tcagtgggtt atcgccgtgg
      481 cgaagcgcca gaaaagtcgc cgggccgtgg caaagggaga taacaaaacg gtcatttttg
      541 attgcccact gtaaagcagc agccacgtcc tggctttctg gtagaccaat aagtgcgcca
      601 tgaccaccag gaacaaagat tgctgcatat tcgctatcag cgttgaggct ggcgacaaca
      661 tccgcgagtt tcttcggatt gcggaacaac gatttatgct gctcaaagaa tggcattact
      721 ttttcatctt tgtgcggcat agcccagtat tcaaacttgg tcatcagacc ggaaatggtc
      781 gccacttcga attcgaaacc tgcagcatgg agatgataca acggcagcaa cgtttcaatc
      841 ggatggttac cggtcgagaa cagttttccg ttatcggtcg gcaaataacg ttcgtccgcg
      901 gcgatcacca gaattttatg tttaccgcga tacggttttg gatagtccac gccatcaaga
      961 tcagagacag gactggtata ttggctaagc gaatattctg aagggaagaa tgcattatct
     1021 tcagcaatat cgacctgcgg atttttactt gtttgaacag tcatagtgta ttccttattg
     1081 ttgcttagtt agggtagtca ctatatttgc gactgagctg gtcgctattt ttaaccagtc
     1141 tggaaaattg agtcaatata tcgaaagcgg tagtcggcgg gtgtaagtat gtaatgatga
     1201 tgctgcactg gtattgaata ttattcacgc caaattttcc ttattgagca acgtcgtcgc
     1261 aagtggcctc aatgatttta gaccattatc aaaggtacgg ggcggcgggg agagatttag
     1321 tgctgtgaaa tattgtggta ctgatgaata tttatatcgc ggattggtag agaaaaagct
     1381 tatttcatgc actatttttg caaatatttc tatatgggag gcggttttgt tgcagattaa
     1441 ttcataaaga aatgcatttg ggtcttggct ggaagagtcg ctggtagatg ttattgtgcc
     1501 agcaggggca gggtaaggtg ctgtatcgat atgctttggc aattgcgcgt gccgcagagg
     1561 gagataaaat gccaataaaa aagcctgagt tcgatgaggg acgaactcag gctagtgtta
     1621 agttttacag taacacgccg cggcgcgttg gctgattaga actggtagac gataccaaat
     1681 gcgagttggt cgtcggtgtt cagtccatga gcgagaacat aatcactctt atcaagcagg
     1741 ttgatctgat aagcggtata tacgttcatg ttcttattga agtaatacca ggtaccgatt
     1801 tcaatgtagt tcagacgatc agaatctttg taacctgcta cgtccagtgc tttagaatag
     1861 gagtaaccga tggatggacg cagaccgaag tcgaactgat attgcagaac agcttcgaag
     1921 ttttgtgttt tgttcacaac atcaccgtcg tcggaattca tgttgtgaga ttcaccgtac
     1981 atcacagcag catagacgtt gttggcatca tattttgttg acagtgccca aacttctgct
     2041 ttttcacctt tactatcact agactggaaa tcaatacgat cagagtttgc gtaagcacca
     2101 gcaaatgaga aaccgtcaat agtgtaagtt acagataaac catggccatc gccatttgct
     2161 ttgtagttgc ctgtaccttc atttttaccc tgatattgca gagcaaaatt tagaccttca
     2221 accatgccaa agaaatctgt gttacgatag gttgcaacac cgttggtacg gttggtcatg
     2281 aacaggtcag taccagccca tgaatcaccg ccccactcaa caaacatatc agtaaatgct
     2341 tctgcatcat acgcgacacc gacattacga ccatagtcga atgaaccaaa atctttgtaa
     2401 ctcaaaccag cataagccaa acgagtttgg tcagggttgt gtctgtttga tgcttccagg
     2461 tctagttcaa actgaccgta accagtcaat tcagggttga tctgagtttc acctttcacg
     2521 cctacacggg cataagaggt atcttgagaa ttatcgcctt tttcaccatt ttcacggtcg
     2581 gtcagaatgc gctcaccaac catcttgccg tacaaatcca gtttattgcc atttttatta
     2641 taaatttcag ccgcatttgc tgcgccagca actaataacg ccgggaccag cattgccaga
     2701 acttttcttt tcattatgta ttcccttgtg attataatct tcatgaatat atcaataagt
     2761 gccgttatcc aaaaaaaagc acatttggat actattctat gaagttcatt ttattttaag
     2821 gattacatgt aacaaatgta tttaaaagat ttcaaaatgt ttctaatcta tttaatataa
     2881 ttcggaatta tgaattctaa atataaatat tcatgaaaat tataaatgta tatcaatatg
     2941 ttatgagttg agcggaaaca tagggggtat caatgattgt tgcgccaaac gactggtgtg
     3001 atcaggcgca cattaatgaa ggcataaaaa aaagccggca tcatgccggc ccaaaaaata
     3061 taaacaagac gataaaaggc taataacgga ggcatcatga cacagaaaaa taaaaaggta
     3121 taattgtatt cagtgctaat atattgatta aatgaattaa taaagccgat ctcaaagata
     3181 aataaaatcg ctcaaaacga tttttcctgt attcactgcc gttgcgcaaa aattatctat
     3241 ttgttcaaaa aatgagtgag tcttgactgg ctcatccaat gtggaaaaat gtgactttta
     3301 tcacataata gtactaagtc tgaattttcc gggttatctc aaaatggaat acggttcgac
     3361 aaagatggaa gagagactct ctcgcagccc tggcggcaaa ctggctttgt gggctttcta
     3421 tacatggtgt ggctatttcg tctgggcaat ggcgcgttac atctgggtga tgagccggat
     3481 ccccgatgcc cccgtgtcgg gatttgaaag cgatttaggc tctaccgcgg gaaagtggtt
     3541 aggggcactg gtcggatttt tatttatggc tctggtcggg gcattgttag gcagcattgc
     3601 ctggtacacc cgacctcgtc ccgcacgtag cagacgctat gaataaaaac tgctaaggtg
     3661 gcgactcttt ggcaacggag cgcgtatgga cttacaacac tggcaggcac agttcgaaaa
     3721 ctggttgaaa aaccatcatc agcaccagga cgcggcccat gacgtgtgtc attttcgccg
     3781 cgtctgggct acggcacaga agctggcggc agatgacgat gtcgatatgc tggtgatttt
     3841 aaccgcctgt tattttcacg atattgtgag cctggcgaaa aatcatccgc aaaggcagcg
     3901 ttcttcaatc ctggcggcag aagagacgcg tcgcctgctc cgtgaagagt tcgagcaatt
     3961 tccggcggag aaaatcgagg ccgtttgtca tgccattgcc gctcacagtt tcagcgcgca
     4021 aatcgccccc ttaacaacgg aagctaaaat cgtccaggat gcggatcggc tggaagccct
     4081 gggggcgatt ggcctggcgc gcgtgtttgc cgtttcaggg gcattgggcg tggcgctgtt
     4141 tgatggtgaa gatccgttcg cacagcatcg cccgcttgat gataaacgct acgcgctgga
     4201 tcatttccag actaagttgc tgaagctacc gcaaaccatg caaaccgcaa gaggcaagca
     4261 gctggcgcag cacaacgcgc attttttagt cgagtttatg gcgaagctca gtgccgaact
     4321 ggcgggggag aatgaaggtg tcgatcacaa ggtgatagat gcgttttcat ccgctggctg
     4381 agcgcgtggc cctaaatggc tgtaattatg ttaacctgtc ggccatctca gatggccggt
     4441 gaaatctatg caggaaaata tatcagtaac cgattcatac agcaccggga atgccgcaca
     4501 ggcaatgctg gagaaactgc tgcaaattta tgatgttaaa acgctggtgg cgcagcttaa
     4561 tggtgtaggt gagaatcact ggagcgcggc aattttaaaa cgtgcgctgg cgaatgactc
     4621 ggcatggcac cgtttaagtg agaaagagtt cgcccatctg caaacgttat tacccaaacc
     4681 accggcacat catccgcatt atgcgtttcg ctttatcgat ctattcgccg gaattggcgg
     4741 catccgtcgc ggttttgaat cgattggcgg acagtgcgtg tttaccagcg aatggaacaa
     4801 acatgcggta cgcacttata aagccaacca ttattgcgat ccggcgacgc atcattttaa
     4861 tgaagatatc cgcgacatca ccctcagcca taaagaaggc gtgagtgatg aggcggcggc
     4921 ggaacatatt cgtcaacaca ttcctgaaca cgatgtttta ctggccggtt tcccttgtca
     4981 gccattttcg ctggctggcg tatcgaaaaa gaactcgctc gggcgggcgc acggttttgc
     5041 ctgcgatact cagggcacgc tgttctttga tgtggtgcgc attatcgacg cgcgtcgtcc
     5101 ggcgatgttt gtgctcgaaa acgtcaaaaa cctgaaaagt cacgaccagg gtaaaacgtt
     5161 ccgcatcatc atgcagacgc tggacgaact gggctatgac gtggctgatg cagaagataa
     5221 cgggccagac gatccgaaaa tcatcgacgg caaacatttt ctgccgcagc accgtgaacg
     5281 catcgtgctg
//
LOCUS       DAMZET010000237         5127 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.237, whole genome shotgun sequence.
ACCESSION   DAMZET010000237 DAMZET010000000
VERSION     DAMZET010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.237"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1168)
                     /locus_tag="POB34_004437"
     CDS             complement(<1..1168)
                     /locus_tag="POB34_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HDI5921275.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMTVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTISIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVW"
     gene            1486..2127
                     /gene="udk"
                     /locus_tag="POB34_004438"
     CDS             1486..2127
                     /gene="udk"
                     /locus_tag="POB34_004438"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDI5921276.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            2219..2800
                     /gene="dcd"
                     /locus_tag="POB34_004439"
     CDS             2219..2800
                     /gene="dcd"
                     /locus_tag="POB34_004439"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HDI5921277.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            2822..4675
                     /gene="asmA"
                     /locus_tag="POB34_004440"
     CDS             2822..4675
                     /gene="asmA"
                     /locus_tag="POB34_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HDI5921278.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4949..>5127)
                     /locus_tag="POB34_004441"
     CDS             complement(4949..>5127)
                     /locus_tag="POB34_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921279.1"
                     /translation="EYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHRVQKVQ
                     IIPLRKDGEMEYEV"
BASE COUNT         1272 a         1282 c         1403 g         1170 t
ORIGIN      
        1 cccagacaac atcgccattg cggttgtagt agcgtttttc cattgaatag gtgtttattt
       61 caccgcttat cagcttttca acctgttgga gatctttatt gagatcctcc ggccaggtca
      121 gttgctgaaa ggtgagtccg cgcagctctt cctgactgta cccgagaaac tggcagagcg
      181 ctttgttggt ttgcagccat tgtccctcgg tgcccactaa cgccataccg atagcggaat
      241 attccatcgc gttccgaaag cgggtttcgc tttcggaaat gtgtttgcgt tccgcacgaa
      301 aggcatacat caccatggtc atgatgttgg cgggcagcag gatcagcaaa aacggtagcc
      361 acggcatatg gctcatcagg tacgtacgcg gcgtagcaag cagggaggga tccgcggcca
      421 tcatcagcga caccatcatc accgtggtaa ggaagatcaa aaaggcttcc attcgtggca
      481 ggcgcacggc gctccacatc aacagcacaa taatgaaagt aaaaggccac ggcagataca
      541 gcatcgaaag ccagcttaac gtcagtgtga tggctaacgt gagcagcgac tcaaaaagca
      601 accgtgggtt gcgatggcgc agcagatagt gtggtttaaa taacaatccc agcggcacca
      661 gtgccagagc gccgatggat tctgacagta cccatatcaa aaatgccctg agaggatcgt
      721 ctccgggcgt cagcaggaca accagaacac cccctaacag aggcggaaca atggcgctgc
      781 cgaatgccag acgcagccag tcagccagat tttgcaaggg gttataccac ggcagcaatt
      841 tacgtagcag cactgccccg accacggctt caacaatact gatggtcgtc caggtcatgt
      901 tcagcgagct ggtggaaaaa agcaggatgg atgcggcgat atttcccagc gagcagctca
      961 gcgcaattcc cggccacatg cgcccggcat ggcgataaaa cgccaccgtc atgatggacg
     1021 tcgggaacca cagtggggcg agttgggtgc caaactgcga aagctcaagc gagaaaaggg
     1081 taaagataaa cgagactaaa cctaaactga ccaggtgaag cagcgggtgg ggcagggcaa
     1141 ttaatacatg ctgtgattgt ttgctcatta ccgctttatc cgcaagacca ggtcgccagt
     1201 attcgtttct gtgtttatgc tagtacaaaa gttttacatt ttatatgcga attgctcata
     1261 aagtgacgta aaggcggata atttgcgcaa ctgcgtataa cattttttac cttgtataaa
     1321 actgatcaac gtaatttgcc accgggaatt cttcgtcaac aataaactct gagcgcgcgg
     1381 cagcgctatt cgactggtat caggcggatg aaatccctat aattgccgcg tttggcgctt
     1441 cgtcgccccc ttcctaacat ccaggttaat caggtcgcta aatatatgac tgatcagtct
     1501 catcagtgcg tcattatcgg tatcgctggc gcatcggctt ccggcaagag tcttattgcc
     1561 agtacccttt atcgtgaatt acgtgagcaa gtcggtgatg aacacatcgg cgtaattccc
     1621 gaagactgct attacaaaga tcaaagccat ctgtcgatgg aagaacgcgt taagaccaac
     1681 tacgaccatc ccagcgcgat ggatcacagc ctgctgcttg agcatttaca agcgttgaaa
     1741 cgcggctcgg caattgacct gccggtttac agctatgttg aacatacgcg tatgaaagaa
     1801 acggtgacgg ttgagccgaa gaaggtcatc attctcgaag gcattttgtt gctgacggat
     1861 gcgcgtttgc gtgacgaact taacttctcc attttcgttg ataccccgct ggatatctgc
     1921 ctgatgcgcc gcatcaagcg tgacgttaac gagcgtgggc gttcaatgga ttcagtgatg
     1981 gcgcaatatc aaaaaaccgt gcgcccgatg ttcctgcaat tcattgagcc ttctaaacaa
     2041 tatgcggaca ttatcgtgcc gcgcggcggg aaaaaccgca tcgcgatcga tatattgaaa
     2101 gcgaaaataa gtcagttctt tgaataagct tgataaattg tgtaccgttc agtgataacc
     2161 tggtatgccc ttgacgtaat tggcgttaag ggagtgatgc gcgaaaggag aaaatgccat
     2221 gcgtctgtgt gaccgagata ttgaagcctg gcttgatgaa ggccgtttgt cgatcaaccc
     2281 acgtccgcca gtggagcgta ttaacggcgc gacggtggat gtacgcctgg gcaataaatt
     2341 tcgtaccttc cgtggtcaca cggcagcgtt tatcgatctg agcggtccca aagatgaagt
     2401 gagcgccgcg cttgaccgcg tgatgagcga tgagatcgtt ctcgacgagg gcgaggcgtt
     2461 ctatcttcac ccaggagagc tggcgctggc ggtgacgctg gagtcggtga cgctgccagc
     2521 cgatctggtg ggctggctgg acgggcgttc ctctttggcg cgtctggggc tgatggtgca
     2581 cgtcaccgcg caccgtatcg atccgggctg gtctggttgc attgtgctgg agttctacaa
     2641 ctccggtaag ctgccgctgg cgctgcgtcc gggcatgtta attggtgcgc tgagctttga
     2701 gccgctttcc ggcccggcgg cgcgacctta caaccgccgt gaagatgcga aatatcgcaa
     2761 ccagcagggc gcggtagcca gccgaatcga taaagactaa ttcaggccca ttgaggatac
     2821 catgagacga tttctgacga cgctgatgat actcctggtc gtgctggtgg ccgggttatc
     2881 tgcgttagtg ttgctggtga atccgaatga tttccgcgac tatatggtca agcaagttgc
     2941 tgcacgtagc ggttatcaat tgcagctcga cgggccactg cgttggcacg tctggccgca
     3001 gcttagtatc ctctccgggc gaatgtctct caccgcccag ggcgcaagcc agccactggt
     3061 tcgcgccgac aacatgcgtc tggacgtggc gcttttacca ctactgagtc atcaactgag
     3121 cgttaagcag gtgatgctaa aaggggcagt gatccaactg acgccgcaga cggaagcggt
     3181 gcgcagtgaa gacgctccgg ttgcaccgcg cgacaatacc ttgccggatc tgtcagacga
     3241 tcgcggatgg tcgtttgata tatccagtct taaggtggcg gacagcgtgc tggtgttcca
     3301 gcatgaagat gacgagcagg tgacaatccg caatattcgc ctgcaaatgg aacaagatcc
     3361 ccaacatcgt ggttcatttg agttctccgg gcgggttaat cgcgatcagc gcgatctcac
     3421 gatatccctt aacggtacgg tagatgcttc tgattatccg catgatttaa cggcggctat
     3481 tgaacaaatt aactggcagt tgcagggtgc agatttacca aaacaaggta ttcaggggca
     3541 ggggagtttc caggcccagt ggcaggagtc acataaacgc ctttcattta accaaatcag
     3601 cttgaccgcc aatgacagca cgctgagcgg gcaagcacag gtcacgctga cagagaaacc
     3661 tgaatggcag ctgagactgc aattcccgca actgaatctt gacaacctca tcccgcttaa
     3721 tgaaacagcg aatggtgaaa acggtgccgc gcagcagggg cagagccaat caacattgcc
     3781 gcgcccggtc atttcttcgc gtattgatga accggcatat cagggactgc aaggctttac
     3841 ggctgatatt ttgttgcagg ccagtaacgt gcgctggcgc ggaatgaatt ttacagatgt
     3901 tgccacgcaa atgaccaaca agtcgggttt gctggaaatt actcaactgc agggcaagct
     3961 taacggtgga caggtttcac tgccgggcac gctggacgcg acatcaataa atccgcggat
     4021 aaacttccag ccacggctgg aaaacgttga gattggtacc attctgaagg cgtttaacta
     4081 tccgatttcg ttgaccggaa aaatgtcact ggctggtgat ttctccggtg ctgacataga
     4141 tgccgacgca ttccgccaca actggcaagg acaggcacat gtcgaaatga ccgacacgcg
     4201 catggaaggg atgaacttcc agcagatgat tcagcaagcg gtagagcgta atggcggtga
     4261 tgtgaaggcc gctgaaaact ttgataacgt gacgcggctt gaccgcttta ccaccgattt
     4321 gacgttgaag gatggcgtcg tgacgttaaa cgacatgcaa ggtcaatcgc ctgtgctggc
     4381 gctgacaggg gaaggcatgt tgaatctggc agatcaaacc tgcgataccc agtttgatat
     4441 tcgggtcgtg ggtggctgga acggggaaag taaactgatt gatttcctga aagaaacgcc
     4501 agtaccgctg cgggtttatg gcaactggca gcaactcaat tacagcctgc aagtggatca
     4561 gttactgcgc aaacatctac aggacgaagc gaaacgtcgc ctgaatgact gggccgagcg
     4621 gaataaagat tcccgcaatg gcaaggatgt gaagaagttg ctggagaaga tgtaggttac
     4681 caggtgacca gtcctgtagg cctgataaga cgcgtaaagc gtcgtatcag gcgttcgtgg
     4741 cggcatcatt atattctgaa tatgtaagat cgaaggctgt tccgttcact tgatgttcct
     4801 cgctactatg gaacgctcaa gccggtgaac gtgccagggt ggctcaatcg ccaccaccct
     4861 ggcaacccgg gctcccggcg gtaaatcgcc gctggcgcgg ttccctcatc ttattccttt
     4921 cggctattgg gtaaaatggc cgataccatc acacctcgta ctccatctcg ccatctttac
     4981 gcagtggaat aatctgcact ttctgcacgc gatggctttc cacctgcaac gttttaagca
     5041 aataatcccc cacctgaact tcttcgccag gttttggaat acgctgtaaa tattccatca
     5101 acagcccggc aatggtgtga tattcac
//
LOCUS       DAMZET010000238         5124 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.238, whole genome shotgun sequence.
ACCESSION   DAMZET010000238 DAMZET010000000
VERSION     DAMZET010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.238"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1223
                     /gene="pgaB"
                     /locus_tag="POB34_004442"
     CDS             <1..1223
                     /gene="pgaB"
                     /locus_tag="POB34_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HDI5921280.1"
                     /translation="GYDMFFTLESGLANASQLDSIPRVLIANNPSLKEFAQQIITVQE
                     KSPQRIMHIDLDYVYDENLQQMDRNIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEV
                     WFPNRLLPMKADIFSRVAWQLRTRSGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQ
                     IHPEQYHRLSPFDDRVRAQVGMLYEDLAGHAAFDGILFHDDALLSDYEDASAPAITAY
                     QQAGFSGSLSEIRQNPEQFKQWARFKSRALTDFTLELSARVKAIRGPHIKTARNIFAL
                     PVIQPESEAWFAQNYADFLKSYDWTAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQA
                     KDKSILELQAQNWQKNGQHQAISSQQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDL
                     IRPEFSTAWYPKND"
     gene            1216..2541
                     /gene="pgaC"
                     /locus_tag="POB34_004443"
     CDS             1216..2541
                     /gene="pgaC"
                     /locus_tag="POB34_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HDI5921281.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            2543..2956
                     /gene="pgaD"
                     /locus_tag="POB34_004444"
     CDS             2543..2956
                     /gene="pgaD"
                     /locus_tag="POB34_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HDI5921282.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(3006..3794)
                     /gene="phoH"
                     /locus_tag="POB34_004445"
     CDS             complement(3006..3794)
                     /gene="phoH"
                     /locus_tag="POB34_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HDI5921283.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(4413..>5124)
                     /locus_tag="POB34_004446"
     CDS             complement(4413..>5124)
                     /locus_tag="POB34_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HDI5921284.1"
                     /translation="TVIHALRDIIKHTPDLLSVRWKREGFISDHAARSKGKETPINLL
                     GFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFRVEFWDRTPLKE
                     QQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPRTAESESSLMLR
                     RGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEYVKPIGGGYFFA
                     LPGVKDANDYLGSALLRV"
BASE COUNT         1338 a         1256 c         1184 g         1346 t
ORIGIN      
        1 tcggttatga catgttcttc acccttgaat caggtttggc aaatgcgtcg caattggatt
       61 ccattccgcg ggtattaatc gccaataatc cctcattaaa agagtttgcc cagcaaatta
      121 ttaccgtaca ggaaaaatca ccacaacgga taatgcatat cgatcttgat tacgtttatg
      181 acgaaaacct ccagcaaatg gatcgcaata ttgatgtgct aattcagcgg gtgaaagata
      241 tgcaaatatc aaccgtgtat ttgcaggcat ttgctgatcc cgatggtgat gggctggtca
      301 aagaggtctg gtttccaaat cgtttgctac caatgaaagc agatattttt agtcgggttg
      361 cctggcaatt acgtacccgc tcaggtgtaa acatctatgc gtggatgccg gtattaagct
      421 gggatttaga tcccacatta acgcgagtaa aatacttacc aacaggggag aaaaaagcac
      481 aaattcatcc tgaacaatat caccgtctct ctcctttcga tgacagagtc agagcacaag
      541 ttggcatgtt atatgaagat cttgccggac atgctgcttt tgatggcata ttgttccacg
      601 atgatgcttt gctttcagat tatgaagatg ccagtgcacc ggctatcacg gcttatcagc
      661 aagcaggctt tagcgggagt ctgagcgaaa ttcgacaaaa cccggagcaa tttaaacagt
      721 gggcccgctt taaaagtcgt gcgttaactg acttcacttt agaacttagt gcgcgcgtaa
      781 aagccattcg cggtccacat attaaaactg cacgaaatat ttttgcactt ccggtaatac
      841 aacctgaaag tgaagcctgg tttgcacaga attatgctga tttcctaaaa agctatgact
      901 ggaccgctat tatggctatg ccttatctgg aaggtgtcgc agaaaaatcg gctgaccaat
      961 ggttaataca attgaccaat caaattaaaa acatccctca ggctaaagac aaatctattt
     1021 tagaattaca ggcacaaaac tggcagaaaa atggtcagca tcaggctatt tcttcgcaac
     1081 aactcgctca ctggatgagc ctattacaac tgaatggagt gaaaaactat ggttattatc
     1141 ccgacaattt tctgcataac caacctgaaa tagaccttat tcgtcctgag ttttcaacag
     1201 cctggtatcc gaaaaatgat taatcgcatc gtatcgtttt ttatattatg tctggtgtta
     1261 tgcatacccc tatgcgtagc gtactttcac tctggtgaac tgatgatgag gttcgttttc
     1321 ttctggccgt tttttatgtc cattatgtgg attgttggcg gcgtctattt ctgggtctat
     1381 cgtgaacgcc actggccgtg gggagaaaac gcaccagctc cccagttgaa agataatccg
     1441 tctatctcca ttatcattcc ctgttttaat gaggagaaaa acgttgagga aaccatacac
     1501 gccgctttag cacagcgtta tgagaacatt gaagttattg ccgtaaatga cggttcaaca
     1561 gataaaaccc gtgccatcct ggatcgcatg gctgcacaaa ttccccattt gcgggtcatt
     1621 catctggcgc aaaaccaggg gaaagccatt gcgcttaaaa ccggagctgc cgcggcgaaa
     1681 agtgaatatc tggtgtgcat tgatggcgat gcgttattag accgcgatgc ggcggcatat
     1741 attgtggaac cgatgttgta caacccgcgt gtgggtgccg taaccggtaa tcctcgtatt
     1801 cgaacacgtt ctaccctggt gggtaaaatt caggttggcg agtattcctc aattattggt
     1861 ttgatcaagc gaacccagcg tatctatgga aacgtattta ccgtttccgg tgttattgcc
     1921 gcatttcgtc gcagcgccct ggcagaagtg ggttactgga gtgacgatat gatcaccgaa
     1981 gatattgata ttagctggaa gctgcagttg aatcagtgga cgatttttta cgagccacgg
     2041 gcactgtgct ggatattaat gcctgaaacg ttaaaagggc tgtggaaaca gcgcctgcgc
     2101 tgggctcagg gcggtgcaga agtattcctc aaaaatatga caaggttgtg gcgcaaagaa
     2161 aactttcgaa tgtggccgct gttttttgaa tactgcctga cgacaatatg ggccttcacc
     2221 tgcctggtcg gtttcattat ttacgcagtc caacttgccg gtgtaccgtt aaatattgaa
     2281 ttgacacata tcgctgcgac acatactgcc ggaatattat tgtgtacgtt atgtttactg
     2341 caatttattg tcagcctgat gatcgagaat cgctatgagc ataatctgac ttcatcgctt
     2401 ttctggatta tttggttccc ggttattttc tggatgctga gcctggcaac gacattggta
     2461 tcatttacac gagtcatgtt gatgcctaaa aagcaacgcg cccgttgggt aagtcccgat
     2521 cgcgggattc tgagaggtta atatgaacaa tttaattatt acgacccgac aatcaccagt
     2581 acgtttactg gttgattatg ttgccacaac catcttgtgg acattatttg cgttgttcat
     2641 attcttattc gccatggatc tgctgacggg ttattactgg caaagcgagg ccagaagccg
     2701 acttcagttc tattttttgc tggcagtggc gaatgccgtc gtgttaattg tctgggcgct
     2761 gtacaataag ctgcgttttc aaaaacagca gcatcatgca gcctaccaat atacgccgca
     2821 agaatatgca gagagcttag caatacctga tgagctctat cagcaactac aaaaaagcca
     2881 caggatgagc gtacacttca ccagccaggg gcaaataaaa atggttgttt cagaaaaagc
     2941 gctagtccgg gcataaacac ccaaaacaaa gcccggttcg cccgggctct gcaccgataa
     3001 cacacttaac tgtaggcatg cagcgtacgt tggcaaagtg ccgaacgtac gcagtcctct
     3061 ttaccgaacc ggacgatccc aaccatttca tcttcttcga aacgttccag cgcgtcactt
     3121 aatccggagc acacgccgcg aggcaaatcg cattgcgtga tatcaccgtt gacgataacc
     3181 gtcacgttct ccccgaggcg ggttaaaaac attttcattt gcgcggcagt cacattctgc
     3241 gcctcgtcaa gaatgacgac tgcattttca aaggtacgtc cacgcatata ggcgaacggc
     3301 gcaatttcca ccttccctat ttccggtcgc aggcagtact gcataaagga agcccctaag
     3361 cgccggacca gcacgtcgta gaccgggcga aaatagggag caaacttttc tgcgatatct
     3421 ccaggtaaga agccaagatc ttcatcggct tgcagaactg gacgggtgac gataatcctg
     3481 tcgacatcct tatgtatcag ggcctctgcc gcttttgctg cgctgatcca ggtttttccg
     3541 cacccggctt cgcccgtggc gaatatcagc tgcttactct caatagcctt cagatagtgc
     3601 aattgcgctt catttcgcgc gaggatgggc gaagtatcgc gactgtcgcg ggccatacca
     3661 atggcttcta cgccgcccat ctgcacaagc gaggtgaccg attcttcttc acgctgctta
     3721 tggctgcgcg aatcccgtct cagcacacgt tttgcctcac gacgagcttt gatcactgct
     3781 ttttgtcttc ccatggagag caccttgagt tgtttgtatt catcacacgc gccgttggca
     3841 gcgcgattat gcgcacgaac atcagagggt tggcttcctt gtaagccata gtttgctttt
     3901 ggataaaatg ccgaaaaacg gctacgcaca ccgtttgcgg cgtcggtaac acgagtaaag
     3961 agaggaagtg agaaaaatac agagtgacga agttgctacc ggaggagaaa tctccgcaag
     4021 tggtggagcg tagcttaaca agacattgtc cagaacagga cgttaccatc cgcgatctcc
     4081 atagtgactg actatcactg ccgggaactt ccgctgctac ttaataatta caacagatct
     4141 cgcattcatt gcaacaatat attcacttat atttaaccat aaaacaccat ttcagtgaca
     4201 ttagtttcta ctggaaagat gacagagtga tgacagatat gaaaaaagct gtgtgctttc
     4261 agcaggattc gcatcaggca tctgcgcacg aacgccggat gcggcgtgaa cgccttatcc
     4321 gcctaccgaa cccgcaccga ttgtaggcat aagaagacgc gcaagcatcg catcaggcat
     4381 ctgcgcaaga acgccttatc cgtctataaa cattaaaccc gcaataacgc gcttccgaga
     4441 taatcgttcg cgtccttcac tcccggcagc gcaaaaaaat aaccgccgcc gataggttta
     4501 acgtattcct ccagcgcttc gccattgagc cttttttgta cagtcaggaa gcctttttcc
     4561 agatcgtgtt ggtaacagac aaacagtagc cccatatcca gttgcccgga gttggtgacg
     4621 cccagtgaat aactgtagcc acgacgcagc atcaggctgg actcactctc cgccgtgcgg
     4681 ggattcgcca gccggatatg gctgtccagc gcaatcacct tcccttccgg atcgctggcg
     4741 taatcgggaa catcatgctc atgctgcatt cccagcggcg caccggtttg cttatcacga
     4801 ccaaaaatcg tctgctgttc tttcagcgga gttctgtccc aaaattccac tcgaaactga
     4861 atcaagcgta ctgcctgata gctgccaccg attgtccacg ctggctcctg ctgatctgcc
     4921 gttacccaca ccactttttg catcaactta tcattctggc tatcgggatt ggcagtgccg
     4981 tctttgaaac ccagcaaatt aatcggcgtc tctttgcctt tactacgcgc cgcgtgatcg
     5041 gaaataaacc cttcccgctt ccagcgcacg ctgagcaaat ctggcgtatg tttgatgata
     5101 tcgcgcagcg catggatcac cgtg
//
LOCUS       DAMZET010000239         5117 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.239, whole genome shotgun sequence.
ACCESSION   DAMZET010000239 DAMZET010000000
VERSION     DAMZET010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.239"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..646)
                     /gene="celF"
                     /locus_tag="POB34_004447"
                     /gene_synonym="chbF"
     CDS             complement(<1..646)
                     /gene="celF"
                     /locus_tag="POB34_004447"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDI5921285.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGK"
     gene            complement(750..1592)
                     /gene="chbR"
                     /locus_tag="POB34_004448"
     CDS             complement(750..1592)
                     /gene="chbR"
                     /locus_tag="POB34_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HDI5921286.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(1600..1950)
                     /gene="chbA"
                     /locus_tag="POB34_004449"
     CDS             complement(1600..1950)
                     /gene="chbA"
                     /locus_tag="POB34_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HDI5921287.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(2001..3359)
                     /gene="chbC"
                     /locus_tag="POB34_004450"
     CDS             complement(2001..3359)
                     /gene="chbC"
                     /locus_tag="POB34_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HDI5921288.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNDLKGIGGNVYNGTLGIMSLMAPFFI
                     GMVLAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(3444..3764)
                     /gene="chbB"
                     /locus_tag="POB34_004451"
     CDS             complement(3444..3764)
                     /gene="chbB"
                     /locus_tag="POB34_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HDI5921289.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(4063..4401)
                     /gene="osmE"
                     /locus_tag="POB34_004452"
     CDS             complement(4063..4401)
                     /gene="osmE"
                     /locus_tag="POB34_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HDI5921290.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            4603..>5117
                     /gene="nadE"
                     /locus_tag="POB34_004453"
     CDS             4603..>5117
                     /gene="nadE"
                     /locus_tag="POB34_004453"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDI5921291.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFT"
BASE COUNT         1407 a         1272 c         1102 g         1336 t
ORIGIN      
        1 atttgccatt taccagcaca tccttaatga acaccatatg gttgaggccg aacagatcga
       61 tagataaatc atcgctgtct ttcagcatca gaacatcgcg aataaacatc ttcatgccga
      121 tcggaatatt acacacgccg ataaagcgtt taaatccggt atgacgataa acggcttcag
      181 tgaccattcc cgccgggtta gtgaagttaa tcacccatgc attcggacaa agttcttcga
      241 catcttttac gatgtcaaaa atcaccggaa tggtacgcag acctttaaac agaccgcccg
      301 cgccgttggt ttcctgacca agataaccat gacttaatgg aatacgttca tccagttcgc
      361 gcgccggtaa ttggcctacg cgcagttggg tagtaacgaa atcagcatct ttcaatgctt
      421 cgcggcgatc cagcgtttta taaagcttca tcgggacgcc agcgttatca atcatccgtt
      481 ggcagagatc gaaaataata tccagtttag ctttgccacc ttcgacatcc accagccata
      541 attcgctgac cggcaattcg tgataacgct taataaatcc ttccagtaac tccggggtat
      601 agctgctccc gccaccaata gtgacgactt ttaatttctg gctcataatt tctcccttca
      661 gtacagaata ctgatatctg acatatctgc cccccggaca taaataatcc agcaacagga
      721 cagatatgtg aattgtcagg tataacgact tactgattaa attcagtcaa tttcttacga
      781 tagctcttag gcgtaaagga cgttaatttc ttaaacgttt taataaacaa actcgggcta
      841 ctataaccgg cctcaaacgc aatatccgtc actgaatagt tggtcatttc cagttgtttt
      901 ttggcaaaat taatacggat ttcattaata atctgcattg gcgttttgcc ataatatcgt
      961 tgagtcgctc gcgtcaaata ttcctgtgat ttggctgaca acgtcaccat attctccagc
     1021 gccgattcac taaactgctc tttatcatgc atcttttcta ccgtactttt cagccactgc
     1081 ggtacatcat caatcacctg ttcttcgcgg taatgacgta aacggttaat gacataaaaa
     1141 gtaaccatct caacaaactc ttccagccct gtttcgcgga aattcaatga agaaatcact
     1201 gtttccacat aggtgagaaa agcgttattg gtccggtata cctgcgaagc tacaaagcaa
     1261 taaggcaaca acggcaggta atgctgctca aaaaagcgtt tactgatccc aacgttcaat
     1321 atacgcgtgg caccaaactc ataaaaactt tgatggtgcg aacctaacgg aataaaaaca
     1381 aaatcgcccc gttccagtaa cacgcgctta ccgttaatct cctggaaata acgcccggtt
     1441 aataccagag taaattcata atagtcgtgc tgatgcagtc cgctgatact ctcagttttg
     1501 ttatagataa acacatggaa gtttttgcca ttaaacaact gctgttctcg ggcagtggca
     1561 atttccggcg cgttaatcac tggctgcatc atcgactcct tatgccttca gtttttcatg
     1621 aagctcaatt aattcagtaa tcagttcacg cgcaagcatg gacgtcatta aatgatcctg
     1681 agcgtggacc agcaccagac tcactttcat cttaccttcg cccgcatcgc cttcaatcag
     1741 tttcgtctgt accagatgcg cttcattcaa tgccatccgt gactgatcca tcatggcttt
     1801 tgctgcggca aaatcgccct gcttcgcctg tttcagtgcc gcatacgcca ggctgcgcgc
     1861 ttgtccggag ttgatgataa gccccatcac cacttcttcc agctcttcag cttctgtttg
     1921 cgtatcggga atattatcga gatccatcat acatcgttcc tctcttttct taccggcacg
     1981 attacccgta ccggcatcga ttaaaatttc agggcgttag cgatatcttc ttcgctctct
     2041 tctttatcaa tcgcgttttg tgctttgtta gccaccacaa caaagggcag ataaattaac
     2101 gttgcgatac caaggttgaa gagtgcaacc agcaatgcgg cgacgctacc gttggtgtta
     2161 aagaaggctc ccagaccggt tggcatggtc cacggtgcaa tattggtcac cggaggaatg
     2221 atgcccatgt agtacgctgc gagggtgatt gccgccagaa tcggttgtac cagtacaaac
     2281 gggataaaca tcaccgggtt catgataatt ggcagaccaa acagaatcgg ttcgttaatc
     2341 tggaagatgc cggacggcag cgccagtttt gccacctgac gataatcagc acggcgagag
     2401 gcgataaaga tagccaggat caggcctaaa gtcgcaccac tgcccccaag gaaaataaag
     2461 gagtccagca tcggcttggc ccagatatgg aaggtcttac cggctgccag cgccgcttcg
     2521 acggaaccat attgctgata ggtcgcgata ttttccagcg cccacggcgt cataatgccg
     2581 ttgtccagtg cggtcagcgc cagcgcgcca tgaataccga agaaccagag cagtggaaca
     2641 aagatcacat aggcccagcc caccacgcta cccaacgatg ccagtggggt tgagatggta
     2701 tccataatga tctgatggaa gttggtgccc caggtattca acgcccaggc aataatcccc
     2761 atcacggaaa gaataataaa gccgggaatt aatgccgaga aggaacgcga tactgaagca
     2821 ggtacgctgt cgggcagttt aatgacccaa ttgcggcgga caataaaggt aaacatttct
     2881 gccaccacca ggccaataat aatcccggag atgatattcg ccccacctaa ccagtttgcg
     2941 ccaaccgcat aggcctcacc gacactatat ggggtgacgg tcataaatgc tgcaacggat
     3001 aacaacccag ccgccagcgc atcgacttta cgctcttctg ccagcaccat gccaataaag
     3061 aacggtgcca ttaaagacat tatccctaat gttccgttat atacgttgcc gccaatacct
     3121 ttcagatcat taagtgtttc aatggttgag gcgtcgaggc gaatacctaa ggaataaaaa
     3181 aacgacccct ccccaaagct tagaaaaacg ttgttaatta atacaaacat ggcccccgca
     3241 agggttaacg gcattaagcg aataaagcca tttttgattg cattaacgtg tggctgcttt
     3301 cctattttaa ctgcaaaagg gaggagtacc ttttcaagcg atgcaataac attactcata
     3361 gaaaaatacc cttaaaaacc gcaatttaaa tattgcggta ttgatttatg aaataactct
     3421 ttgacgggaa aatttaaaat aaattaattt gctgcggctt ttttaatcgc tgcaaccgca
     3481 gccttaagca cgcctaaacc atcgactttg ccataaagca gcgagtcaat tacttcaacc
     3541 ggtttgttgg gtaacaaacg ctggatttcg ggcaacatat aagcaatctg cggccctaat
     3601 aacacgacat cggcattctg acctttttca ccagccagtg tttccggaaa tgcttcaata
     3661 atgaccggaa cttcatattt ttctgcctgt gcgcgcattt ttgataccag taaagaggta
     3721 gacatgcccg cagaacaaaa cagataaatg tgtttctttt ccataaaact gccctcatcg
     3781 acgattatct gtcagccaga cactccgcaa gccttaacct gcttccatgc tctgggtaac
     3841 ttgcgaaacc aacatgactt tttttgtggc tgaaaggagt atacggtata gacctgagca
     3901 atagtatttc tttgacctta taaattgttt ctccttgacc tgtggttatg accctcttca
     3961 catttcgggc aaataattcg cgaagataat taagaactca ggccaaaaaa aaccggcgca
     4021 atggccggtt tccgttgtta ctcaagcttt cagacgaatt gattacttcg cagcctgtgg
     4081 atcagtgtcg tattcagcac aggtctgata accggagttg atgacatgtc cggtatcatc
     4141 taacgcgaca aagtaggttt ctgctttacc atcacgttga cccaggatgt aggtctggca
     4201 agtaccgcga gcatggatca tgctcacttc agacgaaggt ttacccgcaa tttgtgcaac
     4261 ctgcgcccgg ctcatgcctt ttttgacgtc tttcaccaca ggctgtacaa actggtcttt
     4321 ggtacgatca taagccgtac aacccgccag catggttaat accgccgctg cactcagaat
     4381 tcctgccata ttcttgttca tattccgtcc tcttgtttat cagcgtgtta gataagcctg
     4441 gaatacattg ggcgcttttt caagcccgtg aacgaaacgg ctccgctttc agaggattcc
     4501 tgtatgacgt tttaaccacc attcagcccg ctgtcgcttg tcgtttcagt agcaacgggt
     4561 tagctttaag gaagttttgt cttttctgtc tggaggggtt caatgacatt gcaacaacaa
     4621 ataataaagg cgctgggcgc aaaaccgcag attaatgctg aagaggaaat tcgtcgtagt
     4681 gtcgattttc tgaaaagcta cctgcaaact tatccgttca ttaaatcact ggtgctcggg
     4741 atcagcggcg gtcaggactc cacgcttgcc ggaaagctgt gccagatggc gattaatgag
     4801 ctgcgccagg aaactggcaa cgaatcactg caatttattg ccgtacgcct gccctatggt
     4861 gttcaggctg acgaacaaga ttgccaggat gccattgcct ttattcaacc ggatcgcgta
     4921 ttaaccgtta atatcaaggg cgcggtattg gctagcgagc aggcattgcg ggaagcaggc
     4981 attgaactga gcgattttgt ccgtggcaat gaaaaagcgc gtgagcggat gaaagcacaa
     5041 tatagcattg cgggtatgac cagcggtgtc gtggtgggca ccgatcatgc agcagaagcc
     5101 attaccggat tcttcac
//
LOCUS       DAMZET010000240         5117 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.240, whole genome shotgun sequence.
ACCESSION   DAMZET010000240 DAMZET010000000
VERSION     DAMZET010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.240"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..478)
                     /gene="umuC"
                     /locus_tag="POB34_004454"
     CDS             complement(<1..478)
                     /gene="umuC"
                     /locus_tag="POB34_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HDI5921292.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQ"
     gene            complement(478..897)
                     /locus_tag="POB34_004455"
     CDS             complement(478..897)
                     /locus_tag="POB34_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HDI5921293.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            1270..2180
                     /gene="hlyE"
                     /locus_tag="POB34_004456"
                     /pseudo
     CDS             1270..2180
                     /gene="hlyE"
                     /locus_tag="POB34_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            complement(2387..2833)
                     /locus_tag="POB34_004457"
     CDS             complement(2387..2833)
                     /locus_tag="POB34_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HDI5921294.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2925..3584)
                     /locus_tag="POB34_004458"
     CDS             complement(2925..3584)
                     /locus_tag="POB34_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDI5921295.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLATRV
                     L"
     gene            complement(3656..3949)
                     /locus_tag="POB34_004459"
     CDS             complement(3656..3949)
                     /locus_tag="POB34_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HDI5921296.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            4191..4592
                     /locus_tag="POB34_004460"
     CDS             4191..4592
                     /locus_tag="POB34_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HDI5921297.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(4712..5080)
                     /locus_tag="POB34_004461"
     CDS             complement(4712..5080)
                     /locus_tag="POB34_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921298.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
BASE COUNT         1507 a         1238 c         1060 g         1312 t
ORIGIN      
        1 tctgccgctg ccattttttt gccgcatgat tggcaagctt agccaacgtt ttggtctggg
       61 cgatccccac accaacagta agatgggtac gttgtagcac cgttgcgcga atttctctgc
      121 caaaatcagt cagatcgcga caattacgca cacctgtcag atcgcagaat gcctcatcaa
      181 tactgtaaat ctcgacgcgg ggcgatagtt cttccagcgt cgacatcacc cgattgctca
      241 tgtctgcgta aagctcataa ttgctgctaa agcaaaccac gccacagcga cgaaacagat
      301 ctttttgttt gaaccaggga tcgcccattt taacacccag cgcctttgcc tcagcgtttc
      361 gggcgataac gcaaccgtca ttattcgata gcacaaccac cggtttaccc cataaatcag
      421 ggcgaaacac cgtctcacag ctggcataaa acgcgtttac atcacagaga gcaaacatca
      481 gcgcatcgcc ttaacgacgt ggatcaccac accaaagaca tccagcgtat cttcgctact
      541 gatggtaatg ggcgagtacg cactgttcat gggaataagc tgtaccgtcg ggcgtagttg
      601 caattttttc accgtaaact cgccgtcaac agcagcgatg acaatatcac catggctggc
      661 ggtaatagcg ctatcgacaa tcagtaaatc accgtcacta attccaccat caatcataga
      721 atcaccactt gctttgacga agtaagtcgc gctgggatgc tggatcaaca gttgattcag
      781 atcgatgcgc tgttcaacgt aatctgctgc cggtgaagga aagccacact gaacaagatc
      841 gctaaatagc ggaaaagtca caatttcgcg gagatccgca ggcttgataa acaacataat
      901 aatctgcctg aagttatact gtttttatat acagtagtct gttcttgcca gcagatcaat
      961 actgattcag gctatcaata tttgtcgctg catagactgc tgatttttcg ttctcttatc
     1021 ctgtgctcac gtggccttct ggcgacgacg ctcatccagc agaaatgaaa aatatcaccc
     1081 ggcaaaaaaa tagaatagaa gcattcgcca taatgacatt aaacattgtt tgatatttat
     1141 catattaata gaaataaaga cattgacgca tcccgcccgg ctaactatga attagatgaa
     1201 gtaaaattta ttaatagttg taaaacagga gtttcattac aatttatata tttaaagagg
     1261 cgaatgatta tgactgaaat cgttgcagat aaaacggtag aagtagttaa aaacgcaatc
     1321 gaaactgcag atggagcatt agatctttat aataaatatc tcgatcaggt catcccctgg
     1381 cagacctttg atgaaaccat aaaagagtta agtcgcttta aacaggagta ttcacaggca
     1441 gcctccgttt tagtcggcga tattaaaacc ttacttatgg atagccagga taagtatttt
     1501 gaagcaaccc aaacagtgta tgaatggtgt ggtgttgcga cgcaattgct cgcagcgtat
     1561 atttttctat ttgatgagta caatgagaag aaagcatccg cccagaaaga cattctcatt
     1621 aaggtactgg atgacggcat cacgaagctg aatgaagcgc aaaaatccct gctggtaagc
     1681 tcacaaagtt tcaacaacgc ttccgggaaa ctgctggcgt tagatagcca gttaaccaat
     1741 gatttttcag aaaaaagcag cttttccagt cacaggtaga taaaatcagg aaggaagcgt
     1801 atgccggtgc cgcagccggt gtcgtcgccg gtccatttgg attaatcatt tcctattcta
     1861 ttgctgcggg cgtagttgaa gggaaactga ttccagaatt gaagaacaag ttaaaatctg
     1921 tgcagaattt ctttaccacc ctgtctaaca cggttaaaca agcgaataaa gatatcgatg
     1981 ccgccaaatt gaaattaacc accgaaatag ccgccatcgg ggagataaaa acggaaactg
     2041 aaacaaccag attctatgtt gattatgatg atttaatgct ttctttgcta aaagaagcgg
     2101 caaaaaaaat gattaacacc tgtaatgagt atcagaaaag acacggtaaa aagacactct
     2161 ttgaggtacc tgaagtctga taagcgatta ttctctccat gtactcaagg tataaggttt
     2221 atcacattta aaaacacaga cgttttttaa agattgagag ttacctgttg ataataaggt
     2281 atcgggtaac tctcattatg acagagcgct ttcaaaaaca gggctacgaa atggcgagca
     2341 tccagacgta tattaagaaa gcccccatca acatctatta aatttatcac tgcgcccagt
     2401 caggtttatt taagatatga tcctgccagt caatcacttc tgattctttc actgcgatat
     2461 gacgcacaga gatacgttca ccatgcattg ccgctttcga acccgtaagt agcggatgcc
     2521 acgcaggtaa atctttacct tccgccagca accgataagc gcaggtcatt ggcagccatt
     2581 cgaatgttgg cagattttca cgggttaatt taatgcagtc gggttcaaac tcgaaacgac
     2641 gttcgtagtt ccgacattga caggttttaa tattgagctg gcgacaggcg acgttagtga
     2701 agtagatttc gtcggtgtct tcatccatca gtttatgcag gcaacactga ccgcaaccat
     2761 cacacaacga ctcccattcc gcatcgctca tttcgtccag ggttttactt tgccagaaag
     2821 gtacatcgct catcaggtgt tccgccatta cgttaaaacg caccttataa ccagtctggc
     2881 acagcgatgc aagttttgcc gccgctttca ggcggcaaaa agtattacaa aacgcgagtt
     2941 gccaaagaat gcccatcgaa agtgactgtc agctcatcac cgctttgcaa cgggccgacg
     3001 ccatcaggcg tgcctgtcag cacaacgtca ccggccttga gggtaaaaaa cttgctcata
     3061 taagcgatca gcggaacaat tttatggatc atgtccgcag tcgtaccttg ctgacgttgc
     3121 tcgccgttta cgctcaggcc cagcgttgta ttttgcgggt cgccggtaaa ttccgccgcg
     3181 ggaataaacc cggaaagcgg acaagagtta tcaaacgctt tagccttttc ccacggctgc
     3241 ccggctttct tcatttttcc ctgaacatca cgcaatgtca gatcgagcgc cacgccataa
     3301 ccggcaatgg ctttgcggac atgctcttcc gtagcctgac gcagcgtcgc gccaatcaac
     3361 accgccagtt cgacttcatg atgaactgaa ccgaaatcgg atgggatcgc caatggctgc
     3421 cgcagatcgc acagtgccgt ttctggttta ataaacagca ctggctcttc gggcactgcg
     3481 ctgcccatct ctttaatatg tttggcatag ttactgccaa cacagactac tttactcacc
     3541 ggataatcca gcagcgcacc ttgccagttg tgatgttgat acataacgtt cccctcacct
     3601 tatcgtggat atgaaaaccc gcgccgacaa agataatgcc accgccggat atcggttatt
     3661 tgttagtgtc gtctgttttc tgccccatga cggaaagatg ttgcttcagc aaatcttcgg
     3721 gtggtggcgg taactgcaaa tagtaacctt gctcggttaa tgccagttta accttttcaa
     3781 tatcagcatt gaccagtttc ttccgcccat ccaacggcag aatcatcgct aactgaggct
     3841 gaccaaaacc tttcatcagt tcctcaggaa cacgcgaaaa atcgtctttt ttttcgacat
     3901 ataaataggt ctggtcacgc ttgctgcttc gataaatcac acaaaacata cttttactct
     3961 ttaagatgcc aggttttggc ataagctaac atcaatgaaa cccgcgccac aagccagcca
     4021 gctgtagatt tctacggttt gttgagtcca tgccccttta taagtaatcc ccccggcaaa
     4081 aaatgccgcg ccgggatttt accgaacgca gaaaagccac tggaattctc ccgtaaggga
     4141 ttgttatatt ataacagttc atcgtactca ttctgaacag gagactacca atgaaaatca
     4201 agagcatcag taaggctgta ttgctgcttg cactgctaac ctccacgagc tttgctgcgg
     4261 gtaagaatgt caatgttgag ttcagcaaag gccatagcag cgctcagtac tccggcgaaa
     4321 taaagggata cgattacgat acatatacct tctatgccaa aaaaggtcag aaagtgcatg
     4381 tgagtatctc taatgaaggc gctgatactt acctcttcgg gccaggaatc gatgattcag
     4441 ttgatttatc cagatattca ccagaactcg atagtcacgg acaatactca ctccctgctt
     4501 ctggcaaata tgagctgcga gtgctgcaaa cccgaaacga cgcccgtaaa aacaaaacta
     4561 aaaaatataa cgtcgatatt caaattaagt aaatgtcaac cgacggggac tctccccgtc
     4621 ataaacgtta acgccccact cctctctgtc gctcaagagg ttaatgacga atagcagaca
     4681 atgccgaata gaacataatg taaacaaggc atcactcacg acacataaat aacatctttg
     4741 tggtgatttg attgattttt ccactccgct taccatccac accaaaataa cgatcatcac
     4801 gacataattt ttctttaaca tcgcaaaata caccgttagc gaatgtaaat tcgctgggat
     4861 cgtagccttg taatgattgt aagttttcag cggctttttc gccgagatat ttccttgtta
     4921 actctgggga aatgccatct gagttcacac aaatatacgg gtcgcaaaca acgccgcacg
     4981 ccggagattg aagtccgcca gcgtcatgtc cagacgccat tgatgagaac atcatcccca
     5041 cacctgacaa tccgatataa aaaatacgca tcatattcat agcacatcct gataatccaa
     5101 ccgtacaaca cttatgc
//
LOCUS       DAMZET010000241         5107 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.241, whole genome shotgun sequence.
ACCESSION   DAMZET010000241 DAMZET010000000
VERSION     DAMZET010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.241"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(47..772)
                     /gene="pyrH"
                     /locus_tag="POB34_004462"
     CDS             complement(47..772)
                     /gene="pyrH"
                     /locus_tag="POB34_004462"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDI5921299.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            complement(919..1770)
                     /gene="tsf"
                     /locus_tag="POB34_004463"
     CDS             complement(919..1770)
                     /gene="tsf"
                     /locus_tag="POB34_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDI5921300.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            complement(2028..2753)
                     /gene="rpsB"
                     /locus_tag="POB34_004464"
     CDS             complement(2028..2753)
                     /gene="rpsB"
                     /locus_tag="POB34_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDI5921301.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            3121..3915
                     /gene="map"
                     /locus_tag="POB34_004465"
     CDS             3121..3915
                     /gene="map"
                     /locus_tag="POB34_004465"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDI5921302.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            3977..>5107
                     /locus_tag="POB34_004466"
     CDS             3977..>5107
                     /locus_tag="POB34_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDI5921303.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAIL"
BASE COUNT         1228 a         1350 c         1231 g         1298 t
ORIGIN      
        1 cgcgatactt acgcggaatc ttacccttat ttatccatca cgggaattat tccgtgatta
       61 aagtcccttc tttttcaccc attaccacac ggcgcagcgc acccggtttg ttcatgttga
      121 aaacacgaat cggtaattta tggtcacgag ccagcgtgaa ggccgccagg tccatgactt
      181 tcagctcttt ttccagcact tcgctgtaag tcagttgctc gtacatggtt gcagttggat
      241 ctttcgccgg atcagcagta aacacgccgt caactttggt tgctttcagc accacatcgg
      301 cttcaatttc gataccacgc aggcaagctg ctgagtcggt ggtaaagaac gggttacctg
      361 taccggcgga gaggatcacc acacggttgt tgcgcaacag gctgatagct tctgcccagc
      421 tgtagctgtc gcacacgcca ttcaatggaa tagcggacat cagacgagcg ttcacatagg
      481 cgcggtgcag tgcatcacgc attgccaggc cgttcattac ggtcgccagc atccccatgt
      541 ggtcgcccac aacgcggttc atacccgctt tcgccagacc agcgccacgg aacaggttac
      601 ccccaccaat caccacacca acctgaatac ccagttcaac cagttctttg atttcctgag
      661 ccatacgatc cagtatgctt gcatcaatac cgaagccttc agtgccctgc agagcttcgc
      721 cactcaactt aagcagaatg cgtttataga cgggttttgc attggtagcc atgtttcttt
      781 cctgagactg tcaacgattg aaatgaatta atactagcga cattgtacgt cgcttttcag
      841 cgcagcccca caagggttag ctgaatttac aagatgggca caaaaagaag ccgccctcag
      901 gcggctcctt tttgataatt aagactgctt ggacatcgca gcaacttctg ctgcaaagtc
      961 agtctcaact ttctcgatgc cttcacccac ttcgaagcgg atgaagccag tcacttcagc
     1021 gttatgctct ttcagcagct gaccaacagt tttgcttggt tccataacga acggctgacc
     1081 ggtcagagaa acttcgccgg tgaatttctt catgcggcct tcaaccattt tctctgcgat
     1141 ttctttcggc ttaccagact gcatcgcgat atccagctgt acctggtatt ctttttctac
     1201 cacttcagcg gatacgtctt ctggtttgat gaattctggc ttgcttgcag caacgtgcat
     1261 agcgatgtgt ttaaccagct cttcgtcagc gcctttagca gcaaccagaa cgccgatacg
     1321 cgcaccgtgc tgataagaac ccagaacgtc gccttccagc gcagcaacgc ggcgaatgtt
     1381 gatgttttca ccaattttcg ctaccagcgc aacacgttct tcttcgaact gtgctttcag
     1441 aacttcaacg tcagtgattt tgccagcaac agctgcgtcc agaactttgt ctgcgaacgc
     1501 ctggaaacca gcgtcttttg caacgaagtc agtctggcag ttaacttcca gaatgatgcc
     1561 gtagttgccg tcgattttgg ttttgatcac gccgtcagca gcaacgttgc ctgctttttt
     1621 cgctgcttta atagcaccgg acttacgcat gttttcgatt gccagctcga tgtcgccgtt
     1681 agcttcagtc agtgcttttt tgcaatccat catgcctgcg ccagtacgct cacgcagctc
     1741 ttttaccagg gatgcggtaa tttcagccat tctaaaatcc tcggagatgt gatctgcccg
     1801 gcattaaacc gcacagatat aaaagtgaaa aagggggcca tatgcaggcc ccctaaccaa
     1861 acgtgtacta cctggtctat aagggctctc ccccttcata tttcgcgccg cagatgcgtt
     1921 atcttcgctc gctcatcccg gtcacttact gatgtaagct cccgggaatt ctcgagcttg
     1981 ccgcctttct gcaactcgaa ctattttggg ggagttatca agccttatta ctcagcttct
     2041 acgaagcttt cttccgcctg ggaagccaga tcctgagaac ggccttcacg tacggttgca
     2101 gcaacagcgc ccaggtacag ggtcacagca cggattgcgt cgtcgttacc cgggataacg
     2161 aagtcaacac cgtccggatc agagttggta tcaacgatag caaataccgg aatacccagg
     2221 ttgtttgctt ctttgatagc aatgtgttcg tggtcagcat cgattacaaa cagagcgtcc
     2281 ggcagaccgc ccatgtcttt gataccgccc aggctgtttt ccagtttctc cagctcacga
     2341 gtgcgcatca gcgcttcttt cttggtcagc ttttcgaaag taccgtcctg agactgagtt
     2401 tccaggtctt tcagacgttt gatggactga cgaacggttt tccagttagt cagcataccg
     2461 cccagccagc gatggttcac gaagaactgg tcgcagctca gagcagcgtc tttcaccgct
     2521 tcgcttgcag cgcgtttagt accaacgaaa aggattttac ctttgcgaga agcaatcttg
     2581 ttcagttcag ccagagcttc gttgaacatc ggtacagttt tctcaaggtt gatgatgtga
     2641 actttgttac gcgcaccgaa gatgaacggc ttcattttcg ggttccagta acgggtctgg
     2701 tgaccgaagt gaacaccagc cttgagcatg tcgcgcatgg aaacagttgc catgattaaa
     2761 acctctatat aaaagttggg gttatgcctc cacgtatccc atattaccga ccccaaaggg
     2821 caccccggaa tatgtgccga tacgtgtgtg ttgttacaca aagtgagatt gtcgcttccg
     2881 tccagtctgt gtatacgaaa tggatcggaa gtccggcgcg ctttatacca caaatacgtc
     2941 atggacacca ataattgttg gcgctgtgta cagcatcaga tgtcgaattt tctattatag
     3001 aaaaccttca gtggcacgtt tggcgaaatt cagaatgatt ctcaatttgc ccgggtgtga
     3061 taccattgac ggcacttaca tatatattgt cggtatcacc gacgctgatg gacagaatta
     3121 atggctatct caatcaagac cccagaagat atcgaaaaaa tgcgcgtcgc tggccgactg
     3181 gctgccgaag tgctggagat gatcgaaccg tatgttaaac cgggcgtcag caccggcgag
     3241 ctggatcgca tctgtaatga ttacattgtt aatgaacaac acgcggtttc tgcctgcctc
     3301 ggctatcacg gctatccgaa atccgtttgc atctctatta atgaagtggt gtgccacggt
     3361 atcccggacg atgctaagct gctgaaagat ggcgatatcg ttaacattga tgtcaccgta
     3421 atcaaagatg gtttccacgg cgatacctcg aaaatgttta tcgtcggtaa gccgaccatc
     3481 atgggcgaac gtctgtgccg catcacgcaa gaaagcctgt acctggcgct gcgcatggtc
     3541 aaaccaggca ttaacctgcg cgaaatcggt gcggcgattc agaagtttgt cgaagcagaa
     3601 ggcttctccg tcgttcgtga atattgtgga cacggtattg gtcgcggctt ccatgaagaa
     3661 ccgcaggtgc tgcactatga ctcccgtgaa accaacgtcg tgctgaaacc ggggatgacg
     3721 ttcaccattg agccaatggt caacgcgggt aaaaaagaga tccgcaccat gaaagatggc
     3781 tggacggtaa aaaccaaaga tcgcagcttg tctgcacaat atgagcatac tattgtggtg
     3841 actgataacg gctgcgaaat tttgacgcta cgcaaggatg acaccatccc ggcgataatc
     3901 tcgcacgacg aataagatga agccggcgaa tgccggcttt tttaatgcga taatttaatc
     3961 ttatgggtgg cgcacaatga atacccttcc agaacagtac gcaaacaccg ctctccccac
     4021 cctgcccggt caaccgcaaa atccatgcgt ctggccccgt gatgaattaa ccgtcggtgg
     4081 gataaaagcc catatcgata ctttccagcg ttggctgggt gatgcctttg acaatgggat
     4141 ctctgcagaa cagttgattg aggcgcgcac cgagtttatc gaccagctcc tgcaacgatt
     4201 atggattgaa gcgggattca gccagattgc cgacctggca ttggtcgccg tcggtggcta
     4261 cggtcgtggc gagctgcatc cactttctga catcgattta ctgattttaa gccgtaaaaa
     4321 gctcccggac gatcaggcgc aaaaagtggg cgagctgtta acgctgctct gggatgtaaa
     4381 gctggaagtc ggtcatagtg tgcgcacgct tgaagagtgc atgctggaag ggttatcgga
     4441 tttaaccgtc gccaccaatt taatcgaatc ccgcttatta attggcgatg ttgcgctctt
     4501 cctcgaactg caaaaacata tttttagcga aggattctgg ccttccgaca agttctacgc
     4561 ggcgaaagtt gaagaacaga accagcgcca tcagcgttac catggcacca gctacaacct
     4621 tgaaccagac atcaaaagta gccctggcgg cttgcgcgat attcacactc tgcaatgggt
     4681 ggcccgccgt cattttggcg caacatcgct ggatgaaatg gtcgggtttg gcttcttaac
     4741 ctcagcggag cgggcggaat taaacgaatg tctgcatata ctgtggcgta ttcgctttgc
     4801 cctgcatctg gtcgtcagcc gttacgataa tcgtctgtta ttcgatcgcc aacttagcgt
     4861 cgctcagcgt ctgaattaca gtggtgaagg caacgaaccg gtcgagcgga tgatgaagga
     4921 ttacttccgc gttacacgcc gcgtcagtga actcaaccag atgctgctgc aactgttcga
     4981 tgaagccatc ctcgcccttc ccgccgacga aaaaccacgt ccaatcgacg atgagtttca
     5041 gctacgcggt acgctaatcg acctgcgtga tgaaacacta tttatgcgcc agccggaagc
     5101 catcttg
//
LOCUS       DAMZET010000242         5004 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.242, whole genome shotgun sequence.
ACCESSION   DAMZET010000242 DAMZET010000000
VERSION     DAMZET010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.242"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            162..860
                     /locus_tag="POB34_004467"
     CDS             162..860
                     /locus_tag="POB34_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI5921304.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(905..1804)
                     /gene="eamA"
                     /locus_tag="POB34_004468"
     CDS             complement(905..1804)
                     /gene="eamA"
                     /locus_tag="POB34_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HDI5921305.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLMLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            1999..3186
                     /gene="ydeE"
                     /locus_tag="POB34_004469"
     CDS             1999..3186
                     /gene="ydeE"
                     /locus_tag="POB34_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HDI5921306.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            3313..3408
                     /gene="mgtS"
                     /locus_tag="POB34_004470"
     CDS             3313..3408
                     /gene="mgtS"
                     /locus_tag="POB34_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HDI5921307.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(3627..4517)
                     /gene="dgcZ"
                     /locus_tag="POB34_004471"
     CDS             complement(3627..4517)
                     /gene="dgcZ"
                     /locus_tag="POB34_004471"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HDI5921308.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(4772..>5004)
                     /gene="ydeI"
                     /locus_tag="POB34_004472"
     CDS             complement(4772..>5004)
                     /gene="ydeI"
                     /locus_tag="POB34_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HDI5921309.1"
                     /translation="AKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFD
                     GREVQPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         1379 a         1204 c         1061 g         1360 t
ORIGIN      
        1 aaatatcata tcccgcagta gctatacgtc ctggtacatt gatcacagta atacagtgat
       61 aaagttgttc aacatcaggg tgattattaa acgactcggc catacgataa accaatcagg
      121 actcaactcc ttgttttcaa tggctcttaa aaggtgcaaa agtgattttt caaaagattg
      181 cccgcttact taaatccgaa atcaatggca attcatggca tgttggtgat ttgttgccgt
      241 cagaagcgga actcgctgtt cgttataatg tttcgcgtaa taccctgcgt aaggcattat
      301 ccttgctgga aggcgaagga attattcaca gaaagcatgg ttcaggaaca tacattcaaa
      361 aaaagaattt tgttgcacac attgatcaca tgaacagttt cagtgaaatt gcgcataaaa
      421 gcggcaaaga ggcagggagt cagattatga aatttgaagt gcaggatgct tcacccacta
      481 ttgcgactga gctgaattta gtgactggtg agcaggttta ttacataaag agactgcgat
      541 tcattgagga taatgcagca caattagaag aaacgtggat gtcagtagca cgttttcctg
      601 atttaaccgt gtcacatatg caaaaatcca aattttcata tattgagaat gaatgcggga
      661 tcaaaatcat tggcaccttt gaaactttct cccctacttt tcctacccca gaaatcgcca
      721 gtattttacg gatcagccca cgggatccca tacttaaaat tcagacccag gctgtggaca
      781 gtaactctat tccgctggat tattcgttac tttacagcaa tattttcgag ttccaggtaa
      841 agtacttttt cccgcgataa aagcagacgc acagagtgct gacatcacat tctgtgcgta
      901 tggtttagcc tctaaccttt accgctttac gccaccgcaa gccaaataca ttgatataca
      961 gcccggtcat aatgagcacc gcacctaaaa attgcagacc cgttaagcgt tcatccaaca
     1021 atagtgccgc acttgccagt ccgactacgg gcaccagtaa cgataacggt gcaacccgcc
     1081 aggtttcata gcgtcccagt aacgtccccc agatcccata accaacaatt gtcgccacaa
     1141 acgccagata catcagcgac aaaatggtgg tcatatcaat agtaaccaga ctgtgaatca
     1201 tggatgcgga accatcgaga atcagcgagg caacaaagaa gggaatgatt gggattaaag
     1261 cgctccagat taccagcgac atcaccgccg gacgcgttga gtgcgacatg atctttttat
     1321 tgaagatgtt gccacacgcc caactaaatg ctgcagccag ggtcaacata aagccgagca
     1381 tcgccacatg ctgaccgttc agactatctt cgattaacat cagtacgcca aaaatcgcta
     1441 aggcgatccc cgccaattgt ttgccatgca gtcgctcccc gaaagtaaac gcgccaagca
     1501 cgatagtaaa aaacgcctgt gcctgtaaca ccagcgaagc cagtccagca ggcataccga
     1561 agttaatggc acaaaaaaga aaagcaaact gcgcaaaact aatggttaat ccatacccca
     1621 gcagcaaatt cagtggtact ttcggtcgtg cgacaaaaaa gatagccgga aaagcgacca
     1681 gcataaagcg caaaccggcc agcatcagcg gtggcatgtt atgaagcccc actttgatga
     1741 ccacaaaatt tagcccccat acgaccacta ccagtagcgc caacacccca tcttttcgcg
     1801 acattctacc gcctctgaat ttcatctttt gtaagcaatc aacttagctg aatttacttt
     1861 tctttaacag ttgattcgtt agtcgccggt tacgacggca ttaatgcgca aataagtcgc
     1921 tatacttcgg atttttgcca tgctatttct ttacatctct aaaacaaaac ataacgaaac
     1981 gcactgccgg acagacaaat gaacttatcc ctacgacgct ctaccagcgc ccttcttgcc
     2041 tcgtcgttgt tattaaccat cggacgcggc gctacgctgc catttatgac catttacttg
     2101 agtcgccagt acagcctgag tgtcgatcta atcggttatg cgatgacaat tgcgctcact
     2161 attggcgtcg tttttagcct cggttttggt atcctggcgg ataagttcga caagaaacgc
     2221 tatatgttac tggcaattac cgccttcgcc agcggtttta ttgccattcc tttagtgaat
     2281 aacgtgacgc tggtggtgct cttttttgcc ctcattaact gcgcctattc tgtttttgct
     2341 accgtgctga aagcctggtt tgccgacaat ctttcgtcca ccagcaaaac gaaaatcttc
     2401 tcaatcaact acaccatgct caacattggc tggaccatcg gtccgccgct cggcacgctg
     2461 ttggtgatgc agagcatcaa tctgcccttt tggctggcag ctatctgttc cgcgtttccc
     2521 atgcttttca ttcaaatttg ggtaaagcgc agcgagaaaa tcatcgccac ggaaacaggc
     2581 agtgtctggt cgccgaaagt tttattacaa gataaagcac tgttgtggtt tacctgctct
     2641 ggttttctgg cttcttttgt aagcggcgca tttgcttcat gcatttcaca atatgtgatg
     2701 gtgattgctg atggggattt tgccgaaaag gtggtcgcgg ttgttcttcc ggtgaatgct
     2761 gccatggtgg ttacgttgca atattccgtg ggccgccgac ttaacccggc taacatccgc
     2821 gcgctgatga cagcaggcac cctatgtttc gtcatcggtc tggtcggttt tattttttcc
     2881 ggcaacagcc tgctattgtg gggtatgtca gctgcggtat ttactgtcgg tgaaatcatt
     2941 tatgcgccgg gcgagtatat gttgattgac catattgcgc cgccaggaat gaaagccagc
     3001 tatttttccg cccagtcttt aggctggctt ggtgccgcga ttaacccatt agtgagtggc
     3061 gtagtgctaa ccagcctgcc gccttcctcg ctgtttgtca tcttagcgtt ggtgatcatt
     3121 gctgcgtggg tgctgatgtt aaaagggatt cgtgcaagac cgtgggggca gcccgcgctt
     3181 tgttgattta agtcgaacac aataaaaatt taattcagcc ttcgtttagg ttacctctgc
     3241 taatatcttt ctcattgaga tgaaaattaa ggtaagcgag gaaacacacc acaccataaa
     3301 cggaggcaaa taatgctggg taatatgaat gtttttatgg ccgtactggg aataatttta
     3361 ttttctggtt ttctggccgc gtatttcagc cacaaatggg atgactaatg aacggagata
     3421 atccctcacc taaccggccc cttgttacag ttgtgtacaa ggggcctgat ttttatgacg
     3481 gcgaaaaaaa accgccagta aaccggcggt gaatgcttgc atggatagat ttgtgttttg
     3541 cttttacgct aacaggcatt ttcctgcact gataacggat cgttgacaca gtagcatcag
     3601 ttttctcaat gaatgttaaa cggagcttaa actcggttaa tcacattttg ttcgtcaata
     3661 aacatgcagc gatttcttcc ggtttgctta ccctcataca ttgcccggtc tgctcttcca
     3721 atgaccacat ccagaggctc ttcaggaaat gcgcgactca cacctgctgt cacggtaatg
     3781 ttgatatgcc cttcagaatg tgtgatggca tggttatcga ctaactggca aattctgaca
     3841 cctgcacgac atgcttcttc atcattagcc gctttgacaa taatgataaa ttcttcgccc
     3901 ccgtagcgat aaaccgtttc gtaatcacgc gtccaactgg ctaagtaagt tgccagggtg
     3961 cgtaatacta catcgccgat taaatgcccg taggtatcat taaccaattt aaatcggtca
     4021 atatccaaca acattaaata aagattcaga ggctcagtgt tgcgtaactg atgatcaaag
     4081 gattcatcaa gaacccgacg ccccggcaat cccgtcaaaa catccatatt gctacggatc
     4141 gtcagcaaat aaattttgta atcggttaat gccgcagtaa aagaaagcaa cccctcctga
     4201 aaggcgtcga aatgcgcgtc ctgccagtga ttttcaacaa tagccagcat taattcccga
     4261 ccacagttat gcatatgttg atgggcagaa tccattagcc gaacgtaagg taattcatcg
     4321 ttatcgagtg gccccagatg atcaatccac cgaccaaact ggcacagtcc ataagaatgg
     4381 ttatccgtta tttctggctt actggcatct ctcgcgacca cgctgtgaaa catactcacc
     4441 agccactggt agtgggcatc gatagcctta ttgagattta acaagatggc atcaatttcc
     4501 gttgtcttct tgatcattgc cactcctttt tcacagttcc ttgtgcgcgc tattctaacg
     4561 agagaaaagc aaaattacgt caatattttc atagaaatcc gaagttatga gtcatctctg
     4621 agataacatt gtgatttaaa acaaaatcag cggataaaaa agtgtttaat tctgtaaatc
     4681 acctctgcat tatcgtaaat aaaaggatga caaatagcat aacccaatac cctaatggcc
     4741 cagtagttca ggccatcagg ctaatttatt tttatttctg caaatgagtg acccgaacga
     4801 cggccggcgc acttttctta tccagactgc cgctaatgtt gatcatctgg tccggctgaa
     4861 cttctcgtcc atcaaagacg gccgcaggaa taacgacatt aatttcaccg ctcttatcgc
     4921 gaaaaacgta acggtcctct cctttgtgag aaatcaaatt accgcgtagt gaaaccgaag
     4981 cgccatcgtg catgtttttt gcga
//
LOCUS       DAMZET010000243         4994 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.243, whole genome shotgun sequence.
ACCESSION   DAMZET010000243 DAMZET010000000
VERSION     DAMZET010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.243"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..734
                     /gene="fabD"
                     /locus_tag="POB34_004473"
     CDS             <1..734
                     /gene="fabD"
                     /locus_tag="POB34_004473"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDI5921310.1"
                     /translation="LTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCAGVIDFADA
                     VRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVNFNSPGQVV
                     IAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAKITFNAPTV
                     PVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVGPGKVLTGL
                     TKRIVDTLTASALNEPSAMAAALEL"
     gene            747..1481
                     /gene="fabG"
                     /locus_tag="POB34_004474"
     CDS             747..1481
                     /gene="fabG"
                     /locus_tag="POB34_004474"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDI5921311.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            1692..1928
                     /gene="acpP"
                     /locus_tag="POB34_004475"
     CDS             1692..1928
                     /gene="acpP"
                     /locus_tag="POB34_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDI5921312.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            2016..3257
                     /gene="fabF"
                     /locus_tag="POB34_004476"
     CDS             2016..3257
                     /gene="fabF"
                     /locus_tag="POB34_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDI5921313.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            3377..4186
                     /gene="pabC"
                     /locus_tag="POB34_004477"
     CDS             3377..4186
                     /gene="pabC"
                     /locus_tag="POB34_004477"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HDI5921314.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            4189..>4994
                     /gene="yceG"
                     /locus_tag="POB34_004478"
     CDS             4189..>4994
                     /gene="yceG"
                     /locus_tag="POB34_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HDI5921315.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPT"
BASE COUNT         1219 a         1151 c         1410 g         1214 t
ORIGIN      
        1 tgttgactgc atctgttgcg ctgtatcgcg tatggcagca gcagggcggt aaagcaccgg
       61 caatgatggc cggtcacagc ctgggggaat actccgcgct ggtttgcgct ggcgtgattg
      121 atttcgctga tgcggtgcgt ctggttgaga tgcgcggtaa gttcatgcaa gaagccgtac
      181 cggaaggcac tggcgctatg gcggcaatca tcggtctgga tgatgcgtct attgcgaaag
      241 cgtgtgaaga agctgcagaa ggtcaggtcg tttctccggt aaactttaac tctccgggac
      301 aggtggttat tgccggtcat aaagaagcgg ttgagcgtgc tggcgctgcc tgtaaagctg
      361 cgggcgcaaa acgcgcgctg ccgttaccag tgagcgtacc gtctcactgt gcgctgatga
      421 aaccagcagc cgacaaactg gcagtagaat tagcgaaaat cacctttaac gcaccaacag
      481 ttcctgttgt gaataacgtt gatgtgaaat gcgaaaccaa tggtgatgcc attcgtgacg
      541 cactggtacg tcagttgtat aacccggttc agtggacgaa gtctgttgag tacatggcag
      601 cgcaaggcgt agaacatctc tatgaagtcg gcccgggcaa agtgctaact ggcctgacga
      661 aacgcattgt cgacaccctg accgcctcgg cgctgaacga accttcagcg atggcagcgg
      721 cgctcgagct ttaaaagagg aaaatcatga attttgaagg aaaaatcgca ctggtaaccg
      781 gtgcaagccg cggaattggc cgcgcaattg ctgaaacgct cgcagcccgt ggcgcgaaag
      841 ttattggcac tgcgaccagt gaaaatggcg ctcaggcgat cagtgattat ttaggtgcta
      901 acggcaaagg tctgatgttg aatgtgaccg acccggcatc tatcgaatct gttctggaaa
      961 aaattcgcgc agaatttggt gaagtggata tcctggtcaa taatgccggt atcactcgtg
     1021 ataacctgtt aatgcgaatg aaagatgaag agtggaacga tattatcgaa accaaccttt
     1081 catctgtttt ccgtctgtca aaagcggtaa tgcgcgctat gatgaaaaag cgtcatggtc
     1141 gtattatcac tatcggttct gtggttggta ccatgggaaa tggcggtcag gccaactacg
     1201 ctgcggcgaa agcgggcttg atcggcttca gtaaatcact ggcgcgcgaa gttgcgtcac
     1261 gcggtattac tgtaaacgtt gttgctccgg gctttattga aacggacatg acacgtgcgc
     1321 tgagcgatga ccagcgtgcg ggtatcctgg cgcaggttcc tgcgggtcgc ctcggcggcg
     1381 cacaggaaat cgccaacgcg gttgcattcc tggcatccga cgaagcagct tacatcacgg
     1441 gtgaaacttt gcatgtgaac ggcgggatgt acatggtctg accgcgattt gcacaaaatg
     1501 ctcatgttgc gcgcagtctg cgtggttatg agtaataatt agtgcaaaat gatttgcgtt
     1561 attggggggt aaggcctcaa aataacgtaa aatcgtggta agacctgccg ggatttagtt
     1621 gcaaattttt caacatttta tacactacga aaaccatcgc gaaagcgagt tttgatagga
     1681 aatttaagag tatgagcact atcgaagaac gcgttaagaa aattatcggc gaacagctgg
     1741 gcgttaagca ggaagaagtt accaacaatg cttctttcgt tgaagacctg ggcgcggatt
     1801 ctcttgacac cgttgagctg gtaatggctc tggaagaaga gtttgatact gagattccgg
     1861 acgaagaagc tgagaaaatc accaccgttc aggctgccat tgattacatc aacggccacc
     1921 aggcgtaagt gaacatctcc aggcggtcgt tcgaccgcct gagttttatc tttttgtccc
     1981 actagaatca ttttttccct ccctggagga caaacgtgtc taagcgtcgt gtagttgtga
     2041 ccggactggg catgttgtct cctgtcggca ataccgtaga gtctacctgg aaagctctgc
     2101 ttgccggtca gagtggcatc agcctaatcg accatttcga tactagcgcc tatgcaacga
     2161 aatttgctgg cttagtaaag gattttaact gtgaggacat tatctcgcgc aaagaacagc
     2221 gcaagatgga tgccttcatt caatatggaa ttgtcgctgg cgttcaggcc atgcaggatt
     2281 ctggccttga aataacggaa gagaacgcaa cccgcattgg tgccgcaatt ggctccggga
     2341 ttggcggcct cggactgatc gaagaaaacc acacatctct gatgaacggt ggtccacgta
     2401 agatcagccc attcttcgtt ccgtcaacga ttgtgaacat ggtggcaggt catctgacta
     2461 tcatgtatgg cctgcgtggc ccaagcatct ctatcgcgac cgcctgtact tccggcgtgc
     2521 acaacattgg tcatgctgcg cgtattatcg cgtatggcga tgctgacgtg atggttgcag
     2581 gtggcgcaga gaaagccagt acgccgctgg gcgttggtgg ttttggcgcg gcacgtgcat
     2641 tatctacccg caatgataac ccgcaagcgg cgagccgccc gtgggataaa gagcgtgatg
     2701 gcttcgtact gggcgatggt gccggtatgc tggtactaga agagtacgaa cacgcgaaaa
     2761 aacgcggtgc gaaaatttac gctgaactcg tcggctttgg tatgagcagc gatgcttatc
     2821 atatgacgtc accgccagaa aatggcgcag gcgcagctct ggcaatggca aatgctctgc
     2881 gtgatgcagg cattgaagcg agtcagattg gctacgttaa cgcgcacggt acttctacgc
     2941 cggctggcga taaagctgaa gcgcaggcgg tgaaaaccat cttcggtgaa gctgcaagcc
     3001 gtgtgttggt aagctccacg aaatctatga ccggtcacct gttaggtgcg gcgggtgcag
     3061 tagaatctat ctactccatc ctggcgctgc gcgatcaggc tgttccgcca accatcaacc
     3121 tggataaccc ggatgaaggt tgcgatctgg atttcgtacc gcatgaagca cgtcaggtca
     3181 gcggaatgga atacacgctg tgtaactcct tcggcttcgg tggcactaat ggttctttga
     3241 tctttaaaaa gatctaagtt gtcattttcc acccatataa aaggtccgct tgcgggcctt
     3301 ttttcttagc ttttattccg acttgttccg tagtgaacat gctgccacac taacaattct
     3361 ctgataagga gccggtatgt tcttaattaa cggttataag caggaatcgc tggcagtaag
     3421 cgatcgggca acgcagtttg gtgatggttg ttttaccacc gccagagtta tcgacggtaa
     3481 agtcagtttg ttatcggcgc atatccagcg actacaggat gcttgtcagc ggttgatgat
     3541 tttctgtgac ttctggcctc agcttgaaca agagatgaaa acgctggcag cagaacagca
     3601 aaatggtgtg ctgaaagtcg tgatcagtcg cggtagtggc gggcgagggt acagcacatt
     3661 gaacagtgga ccggcaacgc ggattctctc cgttacggtt tatcctgcac attacgaccg
     3721 tttgcgtaac gaagggatga cgttggcgct aagcccggtg cggctggggc gcaatcctca
     3781 tcttgcaggt attaaacatc ttaatcggct tgagcaagta ttgatccgct ctcatcttga
     3841 gcagacaaac gctgatgagg cgctggtcct tgacagcgaa gggtgggtta cggaatgctg
     3901 tgcggctaat ttgttctggc ggaagggcaa tgtagtttat acgccgcgac tggatcaggc
     3961 aggtgttaac ggcattatgc gacaattctg tatccgtttg ctggcacaat cctcttatca
     4021 gcttgtcgaa gtgcaagcct ctctggaaga ggcgttgcag gcagatgaga tggttatttg
     4081 taatgcgtta atgccagtga tgcccgtacg tgcctgtggc gatgtctcct tttcgtcagc
     4141 aacgttatat gaatatttag ccccactttg tgagcgcccg aattagtcat gaaaaaagtg
     4201 ttattgatta tcttgttatt gctggtggta ctgggtatcg ccgctggtgt gggcgtctgg
     4261 aaggttcgcc atcttgccga cagcaaattg cttatcaaag aagagacgat atttaccctg
     4321 aagccaggga ccggacgtct ggcgctcggt gaacagcttt atgccgataa gatcatcaat
     4381 cgtccacggg tttttcaatg gctgctgcgt atcgaaccgg atctttctca ctttaaagcc
     4441 gggacttacc gctttacacc gcagatgacc gtgcgcgaga tgctgaagtt gctggaaagc
     4501 ggtaaagaag cacagttccc tctgcgactg gtagaaggga tgcgtctgag cgattacctc
     4561 aagcaattgc gtgaggcccc gtatatcaag catacgctga gcgacgataa gtacgccacc
     4621 gtagcgcagg cacttgaact ggaaaacccg gagtggattg aaggttggtt ctggccagac
     4681 acctggatgt ataccgccaa taccaccgat gtcgcgttac tcaagcgagc gcacaagaaa
     4741 atggtgaaag cggtcgatag cgcctgggaa gggcgtgcgg acggtctgcc ttataaagat
     4801 aaaaatcagc tggtgacgat ggcatcaatt atcgaaaaag aaaccgccgt tgccagtgaa
     4861 cgcgatcagg ttgcatcggt atttatcaac cgtttacgca ttggtatgcg cctgcagacc
     4921 gacccaaccg tgatttacgg gatgggagag cgttataatg gcaaactttc tcgtgcagac
     4981 ctggaaacgc cgac
//
LOCUS       DAMZET010000244         4960 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.244, whole genome shotgun sequence.
ACCESSION   DAMZET010000244 DAMZET010000000
VERSION     DAMZET010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.244"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..127
                     /locus_tag="POB34_004479"
     CDS             <1..127
                     /locus_tag="POB34_004479"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="RNase BN; member of metallo-beta-lactamase family;
                     the purified enzyme from Escherichia coli forms dimeric
                     zinc phosphodiesterase; in Bacillus subtilis this protein
                     is a 3'-tRNA processing endoribonuclease and is essential
                     while in Escherichia coli it is not; associates with two
                     zinc ions; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDI5921316.1"
                     /translation="KLIITHVSSRYDDKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            315..1534
                     /gene="elaD"
                     /locus_tag="POB34_004480"
                     /pseudo
     CDS             315..1534
                     /gene="elaD"
                     /locus_tag="POB34_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
     gene            complement(1605..3359)
                     /locus_tag="POB34_004481"
     CDS             complement(1605..3359)
                     /locus_tag="POB34_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDI5921317.1"
                     /translation="MRNKNIIMLLMSSLILSGCGPESENKESQQQQPSTPTDQQVLAA
                     QQAAIKEAEQRSVADKATADAKAKALAQQEAQQYSDKQALQGGLQAAPKYQHAAREKA
                     ASQIANPGTARYKQFDDNPVKQVAQNPLATFSLDVDTGSYANVRRFLNHGLLPPPDAV
                     RVEEIVNYFPYDWDIKDKQSIPATKPIPFAMRYELAPAPWNEQLTLLKIDILAKDHKS
                     EELPASNLVFLIDTSGSMISDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALP
                     SISGSHKAEINAAIDSLDAEGSTNGGAGLELAYQQAAKGFIKGGINRILLATDGDFNV
                     GIDDPKSIESMVKKQRESGVTLSTFGVGNSNYNEAMMVRIADVGNGNYSYIDTLSEAQ
                     KVLNSEMRQTLITVAKDVKAQIEFNPAWVTEYRQIGYEKRQLRAEDFNNDNVDAGDIG
                     AGKHITLLFELTLNGQKASIDKLRYAPDNKSVKSDKTKELAWLKIRWKYPQGKESQLV
                     EFPLGPTINAPSEDMRFCAAVAAYGQKLRGSEYLNNTSWQQIKQWAQQAKGEDPQGYR
                     AEFIRLIELADGVTDISQ"
     gene            3497..4468
                     /locus_tag="POB34_004482"
     CDS             3497..4468
                     /locus_tag="POB34_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HDI5921318.1"
                     /translation="MKKINFAFIILFLFSLPLIIFYQPWVNALPPMPRHANPEQLEKT
                     VRYLTQTVHPRSADNIDNLNRSAEYIKEVFVSSGARITSQDVPITGGPYKNIVADYGP
                     ADGPLIIIGAHYDSASSYENDELTYTPGADDNASGVAGLLELARLLHQQVPKTGVQLV
                     AYASEEPPFFRSDEMGSAVHAASLERPVKLMIALEMIGYYDSAPGSQDYPYPAMSWLY
                     PDRGDFIAVVGRMQDINAVRQVKATLLSSRYLSVYSMNAPGFIPGIDFSDHLNYWQHD
                     IPAVMITDTAFYRNKQYHLPGDTADRLNYQKMAQVVDGVINLLYNSK"
     gene            4571..>4960
                     /locus_tag="POB34_004483"
     CDS             4571..>4960
                     /locus_tag="POB34_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HDI5921319.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSE"
BASE COUNT         1425 a         1113 c         1003 g         1419 t
ORIGIN      
        1 taagctaatc attacccacg tcagctcgcg ctatgatgac aaaggttgtc agcacctgtt
       61 acgtgaatgc aggtcaattt tcccggcgac tgaactagcg aatgatttca ccgtgtttaa
      121 cgtttaactc tgcttattat taaaaacagg gcgaaacttg ccctgttatc gcaacccgcg
      181 ctacatatat ccacccacac gggcatattg agcgattttt gatatttatt catctcctta
      241 cgcactatat tcagagcaat aagttgcctc tttttatctt cattcagcac aacctttgca
      301 actaaaagga gcccatgatg gttacagttg tcagcaatta ttgtcaatta tctcaaaccc
      361 aactcagtca gacatttgca gaaaaattta ctgtgaccga ggaattactg cagtctttaa
      421 aaaaaacagc gttatccgga gatgaagaaa gtattgagtt actgcataat attgcgttag
      481 gttatgatga atttggaaaa aaagctgaag atattcttta ccatattgtt agaaacccaa
      541 caaatgagac cctatcgatt atccgactta ttaaaaatgc ctgtttaaaa ttatataatt
      601 tggcacatac cgcaaccaac tcccacctta aaccaactgg tccggataat tcagatgttc
      661 tcctgttcaa aaagctattc tccccttcga aattaatgac aattatcggt gatgaaattc
      721 ctcttatatc tgaaaaacag tcgctttcaa aggtgctttt aaatgatgag aataatgaac
      781 tgagtgatga tacaaacttc tgggataaaa atcgtcaatt aaccacagat gaaatagctt
      841 gctatcttca gaagatcgcc gctaatgcaa aaaatactca agtcaattat cctactggtc
      901 tctacgtccc ctactccacc agaactcacc tggaagacgc tctcaatgaa aatattaaga
      961 gcgatccatc atggccgaat gaagtccagt tattccacat aaatactggc ggacactgga
     1021 tattagtttc gctacagaaa atagtaaata aaaaaataat aaactacaaa taaaatgcgt
     1081 catattcaac tcattgcgtg cactaggcca tgataaagaa aattcactta agcgtgtcat
     1141 taatagtttt aattctgaac tcatgggaga aatgtcgaat aataatataa aagttcattt
     1201 aaatgaacca gagataatat ttttacatgc cgatcttcag caatacttaa gccaaagttg
     1261 cggtgcgttt gtgtgcatgg cagcccagga agtgattgaa caaagggaaa gcaattctga
     1321 cagcgccccc tatacgttat taaaaaacta tgctgacaga tttaaaaaat attcagcaga
     1381 agagcagtac gaaattgatt ttcaacatcg actggtaaac agaaattgtt atttagataa
     1441 atatggcgat gcacgaatca atgcttctta tacacaatta gaaataaaac actcacaacc
     1501 caaaaataga gcatccggca aaagagtgag ttaacatcat tgttaatcaa ttcgaccagt
     1561 cagcaaatat gctgactggt cgaacatagt ttgctaaaca gtcatcactg actgatgtca
     1621 gtcacaccat ccgccagttc aatcaggcga ataaattccg ccctgtaacc ctgtggatct
     1681 tccccttttg cctgctgagc ccactgtttg atctgctgcc aggaggtatt gttcaggtat
     1741 tcagaaccgc gtaacttttg cccatatgca gctactgctg cgcaaaaacg catatcttca
     1801 gagggggcgt ttattgttgg ccccagcggg aattcaacta actgactttc ttttccctgc
     1861 gggtatttcc agcgaatctt taaccaggcc agttctttcg ttttgtccga tttcactgat
     1921 ttgttatccg gggcatagcg taacttatca attgatgctt tttgcccgtt cagcgttaat
     1981 tcgaataaca acgttatatg tttgcctgcg ccaatatccc ccgcatcaac gttgtcgtta
     2041 ttaaaatctt ccgcccgaag ttggcgcttt tcataaccaa tctgacggta ttccgttacc
     2101 cacgcggggt taaactcaat ttgcgctttg acatcttttg ctacggtaat caacgtctgc
     2161 cgcatttcac tattcaatac tttctgcgct tcagagaggg tatcaatgta gctgtagttg
     2221 ccgttaccaa catcggcaat tcgcaccatc attgcctcgt tgtaattgct attccccacg
     2281 ccaaacgtcg acagagtaac accagactcc cgctgttttt tgaccattga ttcaatcgat
     2341 tttggatcgt caatgccaac gttaaagtca ccgtcagtgg ctaataaaat gcgattgatg
     2401 ccgcccttaa taaacccctt cgccgcctgc tgataagcca gttccagccc ggcaccgcca
     2461 ttggtactgc cttcggcatc cagcgaatca attgcggcat taatttccgc cttgtgacta
     2521 ccggagatag aaggcaacgc aatacgggag tcgccagcgt aggtcacgat ggcaatgtta
     2581 tcctgctcac gaagttcttt gaccaataat ttcaacgaag actggataag tggcaaacgt
     2641 tcatcagaaa tcattgaacc agaagtgtcg ataagaaaga ccagattaga agctggtaac
     2701 tcttcacttt tatgatcttt cgccaggata tcaattttca gcaatgttag ctgttcattc
     2761 cacggcgcag gtgccagttc gtagcgcata gcgaaaggta ttggcttagt ggccggaata
     2821 gattgtttgt ctttgatatc ccagtcatac gggaaataat tgactatctc ctccacccgc
     2881 acagcgtctg gcggaggtaa cagcccatga ttgagaaaac ggcgtacatt cgcataactg
     2941 ccggtgtcga cgtcaaggct aaacgtcgcc aacgggtttt gcgctacctg cttaaccgga
     3001 ttatcatcga actgcttgta gcgagcggtt cctggatttg cgatttggga cgctgctttt
     3061 tctctggctg catgttgata ttttggagcc gcctgtaagc ccccctgcaa agcctgtttg
     3121 tctgaatatt gttgcgcttc ttgctgggct aaggcttttg ctttcgcgtc tgcagtggct
     3181 ttatcggcga cggatctttg ctcggcctct tttattgcag cttgttgcgc ggcaagcact
     3241 tgctggtctg tgggggtact gggctgttgt tgctgacttt ccttattctc agattccggc
     3301 ccgcatcctg acaaaatcaa actactcata agcaacatga ttatattttt atttcgcata
     3361 taaaatcctt ttaaataaat acaggcacca gacataaaat atactctttg tataatcctg
     3421 ctaatcttaa taagatgcaa tatcgaaacc cataacaaat aatgattcaa taacgaaacc
     3481 cattaataat ttgcatatga aaaaaataaa ttttgctttt attatattat ttctgttttc
     3541 actccctctt attatttttt accaaccctg ggtcaatgca ttaccgccaa tgccccgaca
     3601 cgcaaatcct gagcaattag aaaaaacagt tcgttatctt acacaaactg tgcatccacg
     3661 tagtgccgac aatattgata atctgaatag gtccgcagag tatataaaag aggtcttcgt
     3721 cagtagcggt gccagaatta cctcgcagga cgtccccatt acgggtggcc cctacaaaaa
     3781 cattgttgct gattatggtc ctgccgatgg accgctgatt attattggtg cgcactatga
     3841 ctctgccagc agttatgaaa acgatgaatt gacctataca cctggcgcgg atgataacgc
     3901 cagcggcgtg gcaggattac tcgaactggc acgtttgtta catcagcaag taccgaaaac
     3961 aggcgtgcag ctggtcgcct atgcgtcgga agaaccgccc ttctttcgta gcgatgaaat
     4021 ggggagcgcg gtacatgcag cttcgcttga gcgtccagta aaattaatga tagcactgga
     4081 gatgattggc tattacgact ctgcgcctgg tagccaggat tacccttacc cggcaatgtc
     4141 ctggctttat cccgatcggg gagactttat tgccgtggtc ggcagaatgc aggatatcaa
     4201 cgccgttcgt caggtaaaag cgacattgtt atcatcccgg tatttatctg tttattctat
     4261 gaatgcccca gggtttattc ccggtattga tttctctgat caccttaatt attggcaaca
     4321 cgatattccc gccgtaatga ttactgacac cgctttttat cgtaataaac aataccactt
     4381 gcccggtgat accgcagaca gattgaatta tcagaaaatg gctcaggtgg tggatggtgt
     4441 tataaatttg ttatacaaca gtaaataata aaaatttctc gaggcgggtc cagaaaaagc
     4501 gacatgcaca ttttattttc cctcgtctgt tttaagatgc gaaacatgaa attcaatgaa
     4561 ggaaaaataa atgggaatga ttggttattt tgcagaaatt gactcagaaa agattaacca
     4621 actgcttgag tctactgaaa aacctttgat ggataatata catgacacgc tttcaggatt
     4681 acgccgactg gatattgata aacgctggga ttttttacac tttgggctga caggcacctc
     4741 tgcttttgat cccgccaaga atgatccgct gagtcgtgcc gtgcttggtg aacacagcct
     4801 cgaagatggc atagatggct tcctgggatt gacctggaat caggagttag ccgcgacaat
     4861 cgaccgactg gaatcactgg atcgaagtga gctgcgtaaa cagttttcca ttaagcgact
     4921 caatgaaatg gagatttatc cgggagtgac cttttcagaa
//
LOCUS       DAMZET010000245         4915 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.245, whole genome shotgun sequence.
ACCESSION   DAMZET010000245 DAMZET010000000
VERSION     DAMZET010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.245"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..624
                     /locus_tag="POB34_004484"
     CDS             <1..624
                     /locus_tag="POB34_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDI5921320.1"
                     /translation="QFAMSGVRDANVRVGDFVVVVGLGAIGQIAIQLAKRAGASVVIG
                     VDPIAYRCDIARRHGADFCLNPIGTDVGKEIKTLTGKQGADVIIETSGYADALQSALR
                     GLAYGGTISYVAFAKPFAEGFNLGREAHFNNAKIVFSRACSEPNPDYPRWSRKRIEET
                     CWELLMNGYLNCEDLIDPVVTFANSPESYMQYVDQHPEQSIKMGVTF"
     gene            642..1430
                     /locus_tag="POB34_004485"
     CDS             642..1430
                     /locus_tag="POB34_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDI5921321.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRGLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            1440..2495
                     /locus_tag="POB34_004486"
     CDS             1440..2495
                     /locus_tag="POB34_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDI5921322.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLRDQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGDIIQQ
                     LVAALYQSAETGTRVEL"
     gene            2492..4759
                     /gene="ycjT"
                     /locus_tag="POB34_004487"
     CDS             2492..4759
                     /gene="ycjT"
                     /locus_tag="POB34_004487"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HDI5921323.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            4756..>4915
                     /locus_tag="POB34_004488"
     CDS             4756..>4915
                     /locus_tag="POB34_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HDI5921324.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESL"
BASE COUNT         1158 a         1235 c         1408 g         1114 t
ORIGIN      
        1 cagtttgcca tgagcggcgt gcgcgatgcc aacgtgcgcg tgggtgattt tgtggtggtg
       61 gtagggcttg gcgcgattgg tcaaatagcc atccaactgg cgaaacgcgc tggcgcgtcg
      121 gtggtgattg gcgtcgatcc tatcgcctat cgctgtgata ttgcccgtcg ccacggtgct
      181 gatttctgcc ttaaccccat tggcactgac gtaggaaaag agatcaaaac gctgaccggc
      241 aagcagggtg ccgatgtgat tatcgaaacc agcggttacg ccgacgcgct gcaatcggcg
      301 ctgcgtggcc ttgcttatgg cggcaccatc tcttatgtcg cgtttgccaa accatttgct
      361 gaaggtttta acctcggacg cgaagcgcat ttcaataacg ccaaaattgt tttctcccgc
      421 gcgtgcagcg aaccgaaccc ggattatccg cgctggagcc gtaagcgtat tgaagaaacc
      481 tgctgggaac tgctgatgaa cggttatctc aattgcgaag atttaatcga cccggtagtg
      541 acctttgcca atagcccgga aagctacatg cagtatgtcg atcagcatcc ggaacagagc
      601 atcaaaatgg gcgttacgtt ttaattcaaa ggaatgaaaa aatgaaaata ggcacacaga
      661 atcaggcatt ctttccggaa aacattctgg agaaatttcg ttatatcaaa gagatgggct
      721 tcgatggttt tgagattgac ggcaaactgc tggtcaacaa tctcgaagaa gtcaaagcag
      781 caatcaaaga aaccggttta ccggtgacca ccgcctgcgg tggctatgac ggttggattg
      841 gcgactttat cgaagagcgt cgtcttaatg gcttaaagca gatcgaacgc attctcgaag
      901 cgctggcaga agtggggggt aaaggtatcg tcgttccggc agcgtggggc atgtttacct
      961 tccgcttacc gccgatgacc tcgccgcgta gcctggacgg cgaccgcaaa atggtgagtg
     1021 attccctgcg cgtactggaa caggtcgccg cgcgtaccgg aaccgtggtg tatctcgaac
     1081 cgttaaaccg ctatcaggat catatgatca acaccctcgc cgatgcccgc cgttacatcg
     1141 tcgaaaacga tcttaaacat gtacagatta tcggcgattt ctatcacatg aatatcgaag
     1201 aagataacct ggcgcaggcg ctgcatgaca accgcggcct gctcggtcat gtgcatattg
     1261 cggataacca tcgctaccag ccgggcagcg gcaccctgga tttccacgcg ctgtttgaac
     1321 agctgcgcgc ggataactat cagggctatg tagtgtacga agggcgtatc cgggcggaag
     1381 atcctgccca ggcgtaccgt gattcgttgg cctggttgcg tacctgctaa gaggtctttg
     1441 tgaaaagtgc aatgacaagc tctccgctgc gggtcgcgat aataggcgca ggccaggtgg
     1501 cggataaggt tcatgcttcg tactactgca cccgcaacga tctggaactg gtggctgtct
     1561 gtgacagccg cctttcccag gcgcaggcgc tggcagaaaa atacgggaat gcatccgtgt
     1621 gggacgatcc gcaggccatg ctgctggcgg tgaaacctga tgtggttagc gtctgctcac
     1681 ctaaccgttt tcattacgaa cataccctga tggcactgga agcgggctgc catgtgatgt
     1741 gcgaaaaacc gcccgccatg acgccagaac aggcgcggga aatgtgcgat accgcgcgca
     1801 aactgggcaa ggtgctggcc tacgactttc accatcgttt tgcgctcgat acgcaacagc
     1861 tgcgtgacca ggtgaccaac ggcgttttgg gagagattta cgttaccacc gcccgcgccc
     1921 tgcgtcgctg cggcgttcct ggctggggcg tctttaccaa taaagaactg cagggtggtg
     1981 gcccgctgat cgacatcggc attcatatgc tggatgctgc gatgtatgtg ctgggctttc
     2041 cggcggtgaa aagcgtgaat gcgcatagct ttcaaaagat cggcacgcaa aagagctgtg
     2101 gtcaatttgg cgagtgggat ccggcaactt atagcgtcga agattcgctg tttggcacca
     2161 ttgaatttca taacggcggc attctgtggc tggaaacgtc atttgcactc aacatccgcg
     2221 aacagtcgat tatgaacgtc agcttttgtg gtgataaagc cggtgcgacg ctgtttccag
     2281 cacatatcta caccgataac aacggtgaat taatgacgct gatgcaacgg gaactggcag
     2341 acgacaaccg ccatttgcgc agcatggaag cctttatcaa tcacgtacag ggcaagcccg
     2401 tgatgatagc cgacgccgag cagggggaca tcatccagca actggtggcg gcgttgtatc
     2461 aatccgcaga aacagggacg cgtgtggaat tatgaccagg ccagtaacgt tatcagaacc
     2521 ccatttcagc cagcataccc tgaacaagta tgcatcgctg atggcgcagg ggaacggcta
     2581 tcttgggctt cgcgccagcc atgaagaaga ttacacgcgc cagacgcgag ggatgtatct
     2641 ggcggggctg tatcatcggg cgggaaaagg tgaaatcaac gaactggtga acctgcctga
     2701 tatcttgggg atggagattg ccataaatgg tgaggttttc tcgttatccc gcgaagcctg
     2761 gcagcgtgaa cttgactttg ccagtggaga attacgccgt agcgttgtct ggcgtaccag
     2821 caacggcaca ggttacacca tcgccagccg tcgctttgtt tcggcagacc aactgccgct
     2881 cattgtgctg gaaatcacta ttacgccact ggacgccgac gcgtcagtgc tgatttcaac
     2941 aggtatcgac gccacgcaaa ccaaccacgg tcgccaacat ctcgacgaaa cccaggtgcg
     3001 ggtgtttggt cagcatctga tgcaggggat ctacaccacc caggatggac gcagtgatgt
     3061 ggccatcagc tgttgctgta tggtgagcgg tgatgtgcag caatgctata ccgccaaaga
     3121 gcgccgtttg cagcaacata ccagtgcgca gcttcatgca ggcgagacag tgacgttgca
     3181 aaaactggtg tggatcgact ggcgggatga caggcaagcc gttttagacg agtggggcag
     3241 cgcgtcgctt cgccagcttg aaatgtgcgc gcagcagagt tacgaccaac ttcttgcagt
     3301 atcaacagaa aactggcgtc aatggtggca gaaacgtcgt atcacggtaa atggcggcga
     3361 agcgcacgat cagcaagcgt tagattatgc gctttatcat ctgcgcatca tgacgcctgc
     3421 ccacgacgag cgcagcagca ttgcggcaaa aggcttaacc ggcgaaggct acaaaggcca
     3481 cgttttctgg gatacagaag tatttttgtt accgtttcat ctgtttagcg atccgacggt
     3541 tgcccgaagt ttactgcgtt atcgctggca caacttgcca ggcgcgcagg agaaagcgcg
     3601 acgcaacggc tggcagggcg cgctatttcc gtgggaaagc gcgcgcagcg gcgaagaaga
     3661 gacgccggaa tttgccgcca ttaacattcg caccgggctg cggcaaaaag tggcctcggc
     3721 gcaggcggaa catcatctgg tggccgatat cgcctgggcg gttattcaat actggcagac
     3781 cacgggggat gaaagtttca ttgcgcatga aggcatggcg ctacttctgg aaactgcaaa
     3841 gttctggatt agccgcgcgg tgagggttaa cgaccgtctg gaaattcatg atgttattgg
     3901 gcctgacgaa tataccgaac atgtcaataa taacgccttc accagctata tggcgtatta
     3961 caacgtccag caggcgctga gtattgcccg ccagtttggc tgtagcgacg atgcgtttat
     4021 ccatcgcgcc gaaatgttcc ttaaagaact gcggctgcca gaaattcagc ccgacggcgt
     4081 tttgccgcag gatgattcgt ttatggctaa gccggcgatt aatctggcga aatacaaagc
     4141 ggcggcgggg aagcaaacca ttctgctgga ttattcacgc gcagaagtga acgagatgca
     4201 aatcctcaaa caagctgatg tggtgatgct caattacatg ctgccggagc agttctcagc
     4261 ggcatcgtgt cttgccaatc tgcaatttta tgaaccgcgc actattcacg actcgtcatt
     4321 aagtaaagca atccatggca ttgttgccgc acgctgtggc ctgctgaccc aaagttatca
     4381 gttctggcgc gaggggactg aaatcgatct tggtgctgat ccgcatagtt gtgatgatgg
     4441 tatccatgct gccgcaactg gcgctatctg gctgggggcg attcagggtt ttgccggggt
     4501 gagcgtgcgt gacggtgaat tgcatctcaa tccggcgtta cctgagcagt ggcaacagtt
     4561 gtctttccct ctgttctggc agggctgcga attacaggtc actcttgacg cgcagcgtat
     4621 tgcgattcga acttctgcgc ccgtttcact gcgtttgaac ggtcagctta tatccgtggc
     4681 tgaagaatct gttttctgtt tgggtgattt tattttgccc ttcaatggga ccgctaccac
     4741 acatcaggag gatgaatgaa actgcaaggg gtaattttcg atctggatgg tgtaatcacc
     4801 gataccgcgc atctgcattt ccaggcgtgg cagcagattg ccgctgaaat tggcatcagc
     4861 attgatgcgc agtttaacga atccctaaaa gggatcagcc gcgatgagtc tctgc
//
LOCUS       DAMZET010000246         4787 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.246, whole genome shotgun sequence.
ACCESSION   DAMZET010000246 DAMZET010000000
VERSION     DAMZET010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.246"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..2960
                     /locus_tag="POB34_004489"
     CDS             <1..2960
                     /locus_tag="POB34_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDI5921325.1"
                     /translation="GSVTHSGYDAAGRLTAQTDAAGRRTEYGLNVVSGDITDITTPDG
                     RETKFYYNDGNQLTAVVSPDGLESRREYDEPGRLVSETSRSGETVRYRYDDAHSELPA
                     TTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGISLYRHYDN
                     RGRLTSVKDAQGRETQYEYNAAGDLTAVITPDGNRSETQYDAWGKAVSTTQGGLTRSM
                     EYDAAGRVISLTNENGSHSDFSYDALDRLVQQGGFDGRTQRYHYDLTGKLTQSEDEGL
                     VTLWYYDESDRITHRTVNGEPAEQWQYDDHGWLTDISHLSEGHRVAVHYGYDDKGRLT
                     GERQTVENPETGELLWQHETTHAYNEQGLANRVTPDSLPPVEWLTYGSGYLAGMKLGD
                     TPLLEYTRDRMHRETVRSFGSMAGSNAAYKLTSTYTPAGQLQSQHLNSLVYDRDYGWN
                     DNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIPYATDPAGNRLPDPELHPD
                     STLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDLIPAGVIRTDDERTHHYHYDSQHRLV
                     FYTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRL
                     TTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQRRSLAEKLQQEGSEDGHGVVFP
                     AELVRLLDRLEEEIRADRVSSESRVWLAQCGLTVEQLARQVEPEYTPARKVHFYHCDH
                     RGLPLALISEDGNTVWSAEYDEWGNQLNEENPYYLYQPYRLPGQQYDEESGLDYNRHR
                     YYDPLQGRYITQDPIGLAGGWSLYAYPLNPVQHVDPLGLSTMIIGNGPVPDNPFGHAA
                     AANRYGLMSSGTGDEMGASVSDYFKKMQPRRDTWIWIIDTTDEEEQCMMNKAIDLNKK
                     LKTINLGPLPVANNCFSRTNMIFEACGFSNPSMNTNAPISLQVLGELYGYQRYFMPKG
                     PFTGFPFEFQEVK"
     gene            2961..3359
                     /locus_tag="POB34_004490"
     CDS             2961..3359
                     /locus_tag="POB34_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921326.1"
                     /translation="MEMLGKILVIIISMIIISCDYYNAGMDYQDNIALEQVVTWDSDN
                     IFTPNIFEHNGRCFMKKNETWMLLNGDATPNIRTMSNKKIECLEKSEVEWASRVCDSI
                     TDKERENLKALICSKPAKSVNTGKRPALGY"
     gene            3423..3977
                     /locus_tag="POB34_004491"
                     /pseudo
     CDS             3423..3977
                     /locus_tag="POB34_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159308.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
     gene            4004..4144
                     /locus_tag="POB34_004492"
     CDS             4004..4144
                     /locus_tag="POB34_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001391868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921327.1"
                     /translation="MSDFDIVSSIDWHDGVLLEVKTTFKESHFNMTLFIDIYKSENIR
                     EK"
BASE COUNT         1351 a         1072 c         1333 g         1031 t
ORIGIN      
        1 acggcagcgt cacgcacagc gggtatgacg cggcaggaag gctcacggcg cagacggatg
       61 cggcgggacg gcggacagag tacggtctga atgtggtgtc aggcgatatc acggacatca
      121 ccacgccgga cgggcgggag acgaaatttt actataacga cgggaaccag ctgacggcgg
      181 tggtgtcccc ggacgggctg gagagccgcc gggaatatga tgaaccgggc aggctggtat
      241 cggagacatc gcgcagcggg gagacagtac gctaccgcta cgatgacgcg cacagtgagt
      301 taccggcgac gacaacggat gcgacgggca gcacccggca gatgacgtgg agccgctacg
      361 ggcagttgct ggcgttcact gactgctcgg gctaccagac ccgctatgaa tacgaccgct
      421 tcggccagat gacggcggtt caccgcgagg aaggtatcag cctttaccgc cactatgaca
      481 accgtggccg gttaacctcg gtgaaagacg cacagggccg tgaaacgcag tatgagtaca
      541 acgccgcagg cgacctgact gccgttatca ccccggacgg caaccggagc gagacacagt
      601 acgatgcgtg gggaaaggcg gtcagcacca cgcagggcgg gctgacgcgc agtatggagt
      661 acgatgctgc cggacgtgtc atcagtctga ctaacgagaa cggcagccac agcgacttca
      721 gctacgatgc gctggaccgg ttggtacagc agggcggctt tgacgggcgg acgcaacgtt
      781 atcattatga cctgaccgga aaactcacgc agagtgaaga tgaggggctt gtcaccctct
      841 ggtactacga tgaatcggac cgtatcactc accgcacggt gaacggcgaa ccggcagagc
      901 agtggcagta tgatgaccac ggctggctga cagacatcag ccacctgagc gaaggccacc
      961 gtgtcgctgt ccactacggc tacgacgata agggccgcct gaccggcgaa cggcagacgg
     1021 tggagaaccc ggagacgggg gaactgctgt ggcagcatga gacaacacac gcctacaacg
     1081 aacaggggct ggcaaaccgc gtcacgccgg acagcctgcc gccggtggag tggctgacct
     1141 acggcagcgg ttatctggcg ggcatgaagc ttggcgacac gccgctgctg gagtacacgc
     1201 gcgaccgcat gcaccgggag acggtgcgca gctttggcag catggcaggc agtaatgccg
     1261 catataaact gaccagcaca tacacccccg caggccagtt acagagccag cacctgaaca
     1321 gcctggtgta tgaccgtgac tacgggtgga atgacaacgg cgacctggtg cgcatcagcg
     1381 gcccgcgaca gacgcgggaa tacggctaca gcgccacggg caggctggag agcgtgcgta
     1441 ccctcgcacc ggacctggac atccgcatcc cgtatgccac cgacccggcg ggaaaccgcc
     1501 tgccggaccc ggagctgcac ccggacagta ccctcacggc gtggccggat aaccgcatcg
     1561 cagaggatgc gcactatgtt taccactatg atgaatacgg cagactgacg gagaagacgg
     1621 accttatccc ggcgggtgtt atccggacgg acgacgagcg gacccaccac taccactacg
     1681 acagccagca ccgtctggtg ttctacacgc ggatacagca tggcgagcca ctggtcgaga
     1741 gccgctacct ctacgacccg ctgggacggc gaatggcgaa acgggtatgg cggcgggagc
     1801 gtgacctgac ggggtggatg tcgctgtcgc gtaaaccgga ggtgacgtgg tacgggtggg
     1861 acggcgaccg gctgacgacg gtacagaccg acaccacgcg tatccagacg gtatatcagc
     1921 cggggagctt cgcgccgctc atccgcatcg aaacagacaa cggtgagcgg gagaaagcgc
     1981 agcgccgcag cctggcggag aagctgcagc aggaagggag cgaggacggg cacggcgtgg
     2041 tatttccggc tgaactggtg cggctgctgg acagactgga ggaagaaatc cgggcagacc
     2101 gcgtgagcag tgaaagccgg gtgtggcttg cgcagtgcgg gctgacggtg gagcaactgg
     2161 ccagacaggt ggagccggaa tacacaccgg cgcgaaaagt tcatttttac cactgcgacc
     2221 accggggcct gccgctggcg ctcatcagcg aagacggcaa tactgtgtgg agtgcagaat
     2281 acgatgaatg gggcaaccag ctgaatgaag agaacccgta ttacctgtat cagccatacc
     2341 gtctgccagg gcagcagtac gatgaggagt cggggctgga ttataaccgt caccggtact
     2401 acgatccgtt gcaggggcgg tatatcaccc aggacccgat tgggctggcg gggggatgga
     2461 gtctgtatgc gtatccgctg aatccagtgc aacacgttga tccattaggg cttagtacta
     2521 tgataatagg taacggcccc gttcctgata atccttttgg gcatgctgcg gcggcaaatc
     2581 gatatggatt aatgagttcg ggaactgggg atgagatggg agcctctgtc tctgattatt
     2641 ttaaaaagat gcaaccccgc agagatacct ggatttggat aatagataca acagatgaag
     2701 aagaacaatg tatgatgaat aaggcaatag accttaataa aaaactaaaa actattaacc
     2761 tcgggcctct tcctgttgca aataattgtt ttagccggac gaacatgatt tttgaggcgt
     2821 gcgggtttag caacccctct atgaatacaa atgcgcctat aagcctacaa gtgctcggtg
     2881 agttatatgg atatcaaaga tattttatgc ctaaaggtcc tttcactgga ttcccatttg
     2941 aatttcaaga ggtaaagtaa atggaaatgc ttgggaagat attagtgata attatttcca
     3001 tgataattat atcttgcgat tattataatg ctggcatgga ctatcaagat aatatagccc
     3061 tcgagcaagt cgtaacatgg gattctgata atatattcac acctaatatc tttgaacata
     3121 atggaagatg ttttatgaag aaaaatgaaa catggatgtt attaaatgga gatgcaacac
     3181 caaacattag aacaatgagc aataagaaaa ttgaatgcct ggagaagagt gaagtagaat
     3241 gggctagtag ggtttgcgac agtatcacag acaaggaaag ggaaaatctg aaggcattaa
     3301 tatgtagcaa accagctaaa tcagttaata caggcaaaag accagcgtta ggatattgaa
     3361 accggcactg cactatatca ctattacaat attctgagtg actagagcaa ctgctccgcc
     3421 atccgggcta gcagtatgac gacgaatccg aactgcacta caactggcag cggtactaca
     3481 atccgggaca gggacgatat atcacccagg acccgatagg gctggcgggg ggattaaacc
     3541 cgtacagtta tgttatgaac aatcctgtgc aatggattga tctattggga ttagctcata
     3601 cttcaggaaa atggaaggac tgtggtaaag gttgtcgaat ccgaatagat ggcgatgctg
     3661 ttggtgacgg aagacatttg cattgggagt gtaaaaatgg gagtggtgta atgggggaat
     3721 ttggaggtat gtcccatggt gaggattact catcagcacc aaatagcatt aagaaatgtg
     3781 caaaaaaata tggtttcgag ccagaaccaa gtaaagatct gtcatgcaaa acctccgggc
     3841 tatcaactac tgatgcggct cttgcaactg gtgctacaat tggcgcaggg tatgttatat
     3901 atagagtgat tcgatttata ccttcacttg caccacctct ttggtggtca atacctgcaa
     3961 acgttgttac tccatgatta aaacggataa tggagattat ataatgagcg attttgacat
     4021 tgtgagttca attgactggc atgatggggt tttgttagaa gtaaaaacga cattcaaaga
     4081 atctcatttt aacatgacgt tatttattga catttataaa agtgagaata ttagggaaaa
     4141 atgatagttg aattttctga tgtagatgat ttagtgttcg ctatggattc tgctgagtta
     4201 atcgataatc acaagtcagg aaatataagt aatgggtata taaagaaatc aaaaaacaaa
     4261 aggatatata aattttttct ttatttttct gatggcttat taagcatgac atttaaaaat
     4321 ttacaattaa taaaaccatt agagtaacca aataaaagga ctggaggaat caggggaaga
     4381 tcattaaagt tgtgattatc atcacaactt taatcttggt tttaccttgt tgatttgatt
     4441 aaaatctaat cgccaaataa ttaaatatat tagaaaagga cgtctggtga acattcaaag
     4501 tcttcttgag catattcgta agcgcctcat ctgaaccgtc tggtatgttt cctccggctc
     4561 cacaaaaata atgtccatca tttttaatgg acactatcgt atggaacagg gaaagatcat
     4621 cagaagttca ctttttgtac taaataattc gcattttatg tttaaaaatt gagatattcc
     4681 ttattacctg aagctgtttt ttattgctta tacatgatca aatactcctt acataaataa
     4741 ggagaacaaa atggaactta aaaaattgat ggaacatatt tctatta
//
LOCUS       DAMZET010000247         4746 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.247, whole genome shotgun sequence.
ACCESSION   DAMZET010000247 DAMZET010000000
VERSION     DAMZET010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.247"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(37..1335)
                     /gene="appA"
                     /locus_tag="POB34_004493"
     CDS             complement(37..1335)
                     /gene="appA"
                     /locus_tag="POB34_004493"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HDI5921328.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGAIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(1415..1507)
                     /gene="cbdX"
                     /locus_tag="POB34_004494"
     CDS             complement(1415..1507)
                     /gene="cbdX"
                     /locus_tag="POB34_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HDI5921329.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(1520..2656)
                     /gene="appB"
                     /locus_tag="POB34_004495"
     CDS             complement(1520..2656)
                     /gene="appB"
                     /locus_tag="POB34_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDI5921330.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARDDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(2668..4212)
                     /gene="appC"
                     /locus_tag="POB34_004496"
     CDS             complement(2668..4212)
                     /gene="appC"
                     /locus_tag="POB34_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HDI5921331.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFALKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(4346..>4746)
                     /locus_tag="POB34_004497"
     CDS             complement(4346..>4746)
                     /locus_tag="POB34_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HDI5921332.1"
                     /translation="DSLLPPPIDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISE
                     ADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIP
                     EVVLAAPEDLVDSAQRLSEVCQWLAEAAPT"
BASE COUNT         1219 a         1332 c         1186 g         1009 t
ORIGIN      
        1 agagcattca ggtaactgaa tgctcttttt tatgcattac aaactgcacg ccggtatgcg
       61 tgcttcattc acgatttgcg taaaacctgc caacgaacac atgccctgcg catttcgctc
      121 ttcacatcct gccagggtca gtttcacctc tccgggcggc gtatttaatg atagcggcgt
      181 tttatcacgc atctgctgta aagtctggaa gaccagcgaa acctgaatcc actggctgtt
      241 atcgctaagc cgacgccagc gttcaaacac cagttcacca cctggcggcg tgttatccgg
      301 ctgaccggga agcgtccagt tgagctccag tgcgccgccg agatttgcca gattagtatc
      361 gtgtccggcg ataaacagca ctgaagtggg taatgtcaca ccatacgcct gtttttgcgg
      421 tggatggggc gtcaacgctg tcttgatcaa atctaataac ggggtggcgc ggctgcgggc
      481 aacctctggc gtgcgttgta gcaaataaaa ttgcgcgtta tgcaaactta gcaaggtgtt
      541 ccactggtgt gaatcggtga tccttcccca ccccggctcc ggcattccct gtgcttgttg
      601 caggagaaat atctccgtca gcattgatgc gaggcttacc gcaccggtta atgagacatt
      661 gtcggcgctc accttgagtt ccgatggtaa tgcctgcgtt aatgaacagc tttcgtcctg
      721 tttctcacgt ttaaggcaca agtttgattg cggaaaatta agcacccgtt ccagttcgcg
      781 aaacgccgtt tgccgatgcc cggtaaagtc agcaattgcc cctcctgccc tgctgaggat
      841 cgcgtcagtc acgttcgcgt tatccagttg gcaaacgcca gtttttagag gattaaataa
      901 cggatcggga ctggacgtat ctgcctgggt atgtacggtt attgcacagt caggtgccag
      961 cccggcggcg aaggcttcgc ctgttttacg ggtacgctcg tcgacatcag caataatcgc
     1021 gacctgacca gactgcgggc agcccttttt cgccagcaat ccgtcggcta ccagacgctg
     1081 gcgttggtaa tgtccgagat aggcgattag ctcaccaccg cgcggtgtca gccaacccag
     1141 ttttaccggc caggttggcc atgcgtctgg ggtgacatcc tgcatcagtt gcgtggcctt
     1201 ggttggagca cgcacaccat gacgactgac aatcaccaca ctttccagct tcagctccgg
     1261 ctcactctga gcgaatgcag attgcggggt taacggaatc agaagagata aaaatgggat
     1321 taagatcgct ttcatcgata tgtttccgct tttcatatct gacattattg attgcctgat
     1381 gcgatgctaa tgcgccgcat caggcctacg ttcgttaact tttcagacgc gggtccagcc
     1441 ataccaacac aagggtggag agcgaacaca tcaacaaaat gccgacgaac caaagtaaat
     1501 accacattgt tcctgttcct tagtacagct cgttttcgtt acggcggaga gtttctgtcg
     1561 tcatgcgtcc ccacattttg tagtagctcc agagagtgta gagcaacaca ataggcaaaa
     1621 atatcagcac gattaccaac ataatgctca gcgtcagctg actggaagtg ctgtcccaca
     1681 acgtcaggct ggagattgga ctcacgcttg acggcatgac aaaggggaac agcgtgatgc
     1741 ctgccgtgaa aatcacgcca aattgcatca atgacgccat caaaaatccc caacccgggc
     1801 gaccacgata aatcgccatc accgtcagca atgggcagaa gaaccccagc agcgggaaga
     1861 tccacaaaac gggcgactcg acaaaattat tcatccaggc accaggtagc actgccacca
     1921 gtttcattaa cggattggaa ggaccgttag catcctgggc gagcagtaca aagccatcaa
     1981 taccgaccca cagccagtaa cccgccagca aaaagcacag catcaccaac agtgcagcgc
     2041 gtttagtcgc cagctgtgaa cgcagatgaa tcacaccaac agttttcagt tgtaaccaga
     2101 cgccaccttg caaaatcacc atcccaaggc tgagcaatcc gcacaataaa gggaatggcg
     2161 tcagcagttg ccagaagctg ccgagatact ccacgcgtaa ttgcggtgtg aaggcaaacg
     2221 gcacgccgag caacaagttg ccgaaggcga taccgaagac taccggcggc accagactgc
     2281 cgatgaccag accggcatcc cacattttac gccagcgtgc atcggcgatt tttccgcgat
     2341 aatcaaaggc cagcgggcgg aagaacagcg agcacagcac caggatcatc gccacataaa
     2401 agccggaaaa cgccgctgca tacactctgg gccaggcggc aaataatgcc ccacctgcga
     2461 ggatcaacca gacctgattg ccttcccagt gcgcaccaac gctattaatc actatccggc
     2521 gttcatcatc atcacgcgcc accagcggca gcaaacagcc aatccccatg tcaaatccgt
     2581 cggagatcat aaagaccacc aggatcacgc caatcagcag ccaccagatg aagcgcaatg
     2641 tttcataatc aaacatgatt ttctccttta cccctgttgc tgcgtcggtt gttcactctg
     2701 catcgcgctc ggccccagac gggcatattt ctgcatcagg tagacttcgg cgattaagaa
     2761 cagggtgtaa agccctacga tcatgatcag tgagaaagcc agttgtcctg tggttaaagc
     2821 ggagtgcgcg gagtatgtcg gtaagatgtc ctgtatcgcc cacggctgac gaccaaactc
     2881 ggtcataaac cacccggctt caatcgcaat ccacggcaac ggcaaactcc agagcgccat
     2941 tttcagcacc cagcgatgct ggtcgatttt gccacgcagc gtctggacaa gcgcaatcag
     3001 catcaccagt agcagcaggg aaccacagcc caccatgatg cggaaactcc agaataccgg
     3061 cgcaacctga ggtatcgcgc cacgcatcgc cgcctggtac tgtgcggctg tgacatgatt
     3121 catatccggc gcatagcggg agagcaacat gccgtagccc aggtcacctt ccagttcccg
     3181 gaatgcctgc aacacatgcg gctcacgatt gccttgcgat atttcctgca ttaacagcca
     3241 ggccatacgt ccgcgttgca agcgtgggta ggtttcagcc atcaaattct tcagacccgg
     3301 cacgggttta tctaatgagt gagtggcgag gatccctagc agcgcgggaa ttttgagggc
     3361 aaaggcgtta cgctcttgat cctgttccgg ccaggcaacc acatggaacg gtgcaggtgc
     3421 aggttccgtt tgccactccc cttccatcgc cgccagtttt accggttgta cttgcgcgac
     3481 ttcatacgca gaactgtctc cgagttgcag ggtaccgata atcgccagag taccgaagac
     3541 ggaaccgatg gcaaacgagc gtaatgcgac atcgcgctcc cgtccgcgca gtaaatacca
     3601 ggcgctgatc gccataataa acatggcccc ggtcacgtag cccgccatta cggtgtgcac
     3661 aaatttcacc tggctgaccg gattaaagac cagctcgctg aagctggtca tctccatacg
     3721 cagggtgtcg atatcaaaat gcgcaccggt cgggtattgc atccaaccat tggcattcaa
     3781 tatccacaac gcagagagat ttgaaccgaa cgccaccagc cacgtcacca gcaggtgctg
     3841 gtatttattc agccgttgcc agccgaagaa gaacagcccg acaaaggtgg attcgaggaa
     3901 gaaggccatt aatgcttcca tcgccagtgg tgcgccaaaa atatcgccca catagttgga
     3961 atagaatgac cagttagtac caaactgaaa ctccatggtc aggccggtag ccacgccaag
     4021 agcaaaattg ataccgaaga gcttacccca gaagcgcgtc atatcgcggt agattgtttt
     4081 gccggtgacc acgtaaatgg tttccatgat agccagcaaa aaaatcagcc ccagggtaag
     4141 gggtacaaat aaaaagtgat acagcgcggt cagagcaaac tgccagcgcg ataaatcaat
     4201 gacatcccac atgcgcactc ctgtaggcga agagagaaaa ataacccgat aatggtagat
     4261 ctccctcttt atcctgaaac tctacgacca gaggtattcg ccgattgcga tattcataag
     4321 actgaaagcg atacttaccg tctttttacg tcggtgcagc ttccgccagc cactgacata
     4381 cctcgctaag ccgctgcgca gagtcgacca aatcttctgg cgctgccagc accacttccg
     4441 gtattgggca gatttcataa ctttccagta acgggccttt taaggtgtcc gtacagcgta
     4501 aatgccagac atggcgtaac cccgtggcgt tgatatagct ctcgccatag ccaatggtac
     4561 gaatctgaat atttcccggc ccacagagac gtgagagaaa aagccgatca gcctcgctga
     4621 tgggtaattg cgtcagatta atgctgtgcg gctgcgcgtc ggggttacgt acatgtgcca
     4681 gtaactcatg cgccaacggt aggccattca tcaggccatc aatgggcggc ggtaacagcg
     4741 aatctg
//
LOCUS       DAMZET010000248         4703 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.248, whole genome shotgun sequence.
ACCESSION   DAMZET010000248 DAMZET010000000
VERSION     DAMZET010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.248"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(62..1451)
                     /locus_tag="POB34_004498"
                     /pseudo
     CDS             complement(62..1451)
                     /locus_tag="POB34_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567368.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            complement(1761..1910)
                     /locus_tag="POB34_004499"
                     /pseudo
     CDS             complement(1761..1910)
                     /locus_tag="POB34_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2088..3281)
                     /locus_tag="POB34_004500"
     CDS             complement(2088..3281)
                     /locus_tag="POB34_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDI5921333.1"
                     /translation="METTSVIILNGFLGAGKTTLLKNLLIQAHEKQLSVSVIVNDMSE
                     LDVDGVLIANTDIVDAKDNNFVSITADSISSPAGLKKMDLAINHLLDHNKPDVMLIET
                     SGSSHPLPLVKYLRRHSRVRLKAFLTLVDTVMLHEDYNDGKKLIPTLQENLRHNKRGL
                     ENLLAEQIMFCNRLLLTRNDRLPFDIISAVAKAIHPLNPSVDVLAVSWGNIELSTLLA
                     IPDYNFDRVELLISELEALVGNMDTPCNNEELIWRVIRDDRPFHPQRLWDTCHRFMGM
                     GVYRSKGFFWLPGRDDLALLWNQSAGSISLALIGYWKAGVLEHTDNNLTREERSALQR
                     HIDTASGRFGDRCCQLTIIGNATEVNDFTHALSLCLLTEEEIQWWMSGGVFPDPWPQK
                     VTRLS"
     gene            complement(4504..>4703)
                     /locus_tag="POB34_004501"
     CDS             complement(4504..>4703)
                     /locus_tag="POB34_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI5921334.1"
                     /translation="VKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRS
                     PREYLRQRACNGLSDNRCLEI"
BASE COUNT         1173 a         1102 c         1101 g         1327 t
ORIGIN      
        1 aatagccgaa agatagcgca cacatcgccg ttggtcagtt agtacgaggt ttccgccaag
       61 ttcacctttt accaaagatg gcctcagagc ttccctgaag ctttatcatg gcttcaagta
      121 ggtcatcttc gttcagtgag cgctgagatt tgttgatgct aggggcgtgc cgtctgcggg
      181 aaggctcatc accagaacaa ggtaacgatt gtgagcgatt attgtcccgt tttctctgga
      241 cctgacgggc aacatccaac acatggccaa gtctcttgtt atctacaatg gcgacgggat
      301 caacctcaga aagccggtca taggtggagt agggcagcag cacgccattt agtcgcagct
      361 ccttatgtcc gtcaggataa tgccagacat caatatactt gccgatggct cttcgactgt
      421 actcgttgtc ctcaatcaga tagagcattt tatcgtattg tacagtcaga ttttttgata
      481 cccggcgagg ctctcgctag gtaaattctg catcaaggtc atcgtctaat gccagcggcc
      541 ggtgcgcgtt aaaatcatgc aagggttctt tggcaaagcg ccggttataa tccttctgca
      601 aatgcattgg ccgcttctat tgtacagatc cccttaagcc tgagctcctt aacgagtcgg
      661 tcctgaagtg tcaaatgagc acgttcaacg cggccttttg cctggctggt ttcagcacac
      721 aaaggagtaa tattaaggca gcgcatggcc ctggcaaact gggtttcgcc ggggccggtg
      781 gtggcgtgct tattattgac tctgaatacg ctggctttat cgctgtagag gatcatcggt
      841 ttaccgtatt tctcgatata gccacgtgta gcttcaaaat aggtaaatgt ggactcagat
      901 ttcacaaaca gcaggtgcat caggcgactg gtggcatcgt caacatagac cagggcagaa
      961 cagggtcgac cccggttttc aaaccagtgg tggtcgcagc catcaatttg tatcagctcg
     1021 ccagtacaag gacgccggta acgaggctga tgaattttcg gtgcgcgttg tctgcgggga
     1081 atccagagtc cggcctttat catgagacgt cggatggttt cctttccaag gaccagaccg
     1141 tgaacttcct ccagcttttc ccgcgctagc gtggggccaa aatcacggta gtgctcccgg
     1201 attatactga gtgcctgatc tgtcagtgac gctggcaaca gacggttacc agtgcggcca
     1261 cgactctggt tgttcatgcc aagcggccca tactggcgat aacgtttgag cagacggctg
     1321 caatgacgtg gcgtgatgcc cagcatttcg gcagcctgtc taggacggat attgcgatcg
     1381 atgacatcct gaaggatttt gaggcgattg atttctttca cggtgaacat tccggcgtca
     1441 tttatagcca tagaaaactc ctcatcagga aaaagtgagt cgttgtctag acatagggat
     1501 ctgagaaact atagcctgga cattcctaat gagccacaaa tagacattag cattgagcct
     1561 ctacaatgtt tgtgcgcaca atctatctta tgttaaataa cattgaatat taacttactt
     1621 catgaagctt actgcttcta tttccacttc tttttcttat taatcgctcc cctaatccac
     1681 gcattcatct gctctacagg ctctaaaata ttttttctga gtactgagca caaactgctc
     1741 cataaaaaac agaccacaag tttgggcatg atactgatgt agtcacgttt tattgttttc
     1801 atgaagctct ctgctattcc attactcacc ggactccgca ccgccgtgtt cttcgattta
     1861 agtcccaaca tccgagcgaa ctggcgtgtt tcattaaccc ggtagtcaga gtcagcggtc
     1921 gaaagacctc ccaggtagct actctgcata cccactataa tgcaactatt ctcgtgacgt
     1981 gtgtttcagc tctcccccgg ctatgatacg agatggtatg gagagccctt ctggggccac
     2041 tgcctgcacc agatgcaaca ggacgcctga tatgaatgca atttctgtca ggacaggcgg
     2101 gtaactttct gtggccatgg atcagggaat actccacccg acatccacca ctgaatttct
     2161 tcttcagtca acaagcaaag ggataaggca tgtgtaaaat cattaacttc agtggcattg
     2221 ccaatgatag tcaactgaca gcagcgatcg ccaaatcgcc cagaggctgt gtcgatgtgt
     2281 cgctgtagcg ctgaacgttc ttcacgagtt aaattattgt cagtatgttc cagtactcca
     2341 gctttccagt agccaatgag cgccagactg atgcttcctg ctgactgatt ccagagcagg
     2401 gcaagatcat ctctcccggg gagccaaaaa aaacctttac tgcgataaac gcccatgccc
     2461 ataaagcggt gacaggtatc ccataatctt tgcggatgga atggcctgtc gtccctgatg
     2521 acgcgccata taagttcctc attgttgcac ggagtatcca tgtttcctac aagtgcttcc
     2581 agttcactga tgagtaactc aacacggtca aaattgtagt ccgggatcgc cagtaatgtg
     2641 ctcagttcaa tatttcccca tgataccgcg agcacatcca cggaggggtt aagtgggtgt
     2701 atggcttttg ccacagcgga gataatgtca aagggcaggc ggtcatttct ggtcagcagc
     2761 agcctgttgc agaacatgat ttgctcggcc agcaaattct ccaggccgcg tttgttatgc
     2821 cggagatttt cctgaagagt ggggatcagt tttttcccat cattgtaatc ttcatggagc
     2881 atgacagtat caacgagtgt taaaaacgcc ttcaggcgta cacgtgaatg gcgacgcagg
     2941 tatttgacca gaggaagcgg atgactgctt ccggaggttt caatcagcat cacatccggc
     3001 ttgttgtgat caagcaggtg gttaattgcg agatccatct ttttgaggcc tgcggggctg
     3061 ctgatgctgt ctgcggtgat acttacgaaa ttattgtctt ttgcatctac aatatctgtg
     3121 ttggcgatca gcacgccgtc cacatccagt tcgctcatgt cattgactat gacagagact
     3181 gaaagctgtt tttcgtgtgc ctgtatgagc aggtttttca gcagagtggt tttccctgcg
     3241 ccgaggaagc cattgagaat gatgacagat gttgtttcca tatcagcagt acaccagtta
     3301 gttagcgagg aaggcgcttg gtggtacaaa gagcatccgg attgaatcgc ataacgtcga
     3361 cgggagttgt tgtcgttttt cctgtgaggt ctcaacctgc cacagaacgc accggactta
     3421 ttggtattta gtctccatca gcataattat caccattttt atctccgggg gaagatttgt
     3481 tctgaacagg agtgggaggt gtgacctcct gaaagtaatt acagattgct agttgttgta
     3541 tttttattcg aatatgtctc ttcgtatcag tgatagcacc tagataaagt atattccggg
     3601 agcgttatct gttctagata tcagagagtc agtcatttgt aaaaaagaga gctgtatcat
     3661 tctatttttc ctgtctattc accctgttgt cgtatctaaa agacatccgt tccgaggatg
     3721 tcgggcaccc gttctgtact aagatacata gcttttgggt ctgcactatt tggaaccgac
     3781 agaagtctgt ctgtgatatc cgcatttttc tgtgtgcgat atattccgaa aaacttcaaa
     3841 ccacttaaaa gaggtgtttc cggaggataa gttcgaccag ggaatagtgt ttcctggtaa
     3901 gtgtggcgga gccagttcac caggagtatc tttcttatcc agttatactg gattttatta
     3961 gatgatcacc ctaccctgca gcagtacgct gacggtgttt ttttggagaa ccattatgaa
     4021 taacgtcatc tttgattggt atttatctag gcaaaaattc attcaacgtc cactgccaag
     4081 atgcaacagg cagagtcctt aactgtcaga atccaagtgc tcgaaattat tcgaattcct
     4141 tactaattcc tcctacacaa ctattgttat ggaagcttgc gaagatgctc actatatggc
     4201 acggaaattt tcggaaatgc gtcataaaac aaagctaata cctcctcatt ttgtccaacc
     4261 cttcgttaaa attaataaaa atgatttctt tgaggctgaa accatatgtg aagttacatc
     4321 aagccatcaa tgagatttgt agcattagga gctgaatccc agcagaggat tccacttttc
     4381 atccgtcgtt gacaatggcc ttgaaaaacg ccgttactca ccgttctcct tcacggtaat
     4441 acgcagttat agttgcagag caatgtaatg gatcttttat attaagaaat tgtggatttg
     4501 cccctatatt tccagacatc tgttatcact taacccatta caagcccgct gccgcagata
     4561 ttcccgtggc gagcgataac ccagcgcact atgcggatgc cattcgttat aatgctcgaa
     4621 cgcctctgca aggttctttg ctgccgttaa cccgtctggt ttgggcatga tactgatgta
     4681 gtcacgcttt atcgttttca cga
//
LOCUS       DAMZET010000249         4693 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.249, whole genome shotgun sequence.
ACCESSION   DAMZET010000249 DAMZET010000000
VERSION     DAMZET010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.249"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..565
                     /locus_tag="POB34_004502"
     CDS             <1..565
                     /locus_tag="POB34_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDI5921335.1"
                     /translation="AQDLVDTLDALQIDKATFIGHSMGGKAVMALTALAPDRIDKLVA
                     IDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQFLLKSFVDG
                     EWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLLAQFPQARAH
                     VIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            705..998
                     /gene="ybfE"
                     /locus_tag="POB34_004503"
     CDS             705..998
                     /gene="ybfE"
                     /locus_tag="POB34_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HDI5921336.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            1138..1668
                     /gene="fldA"
                     /locus_tag="POB34_004504"
     CDS             1138..1668
                     /gene="fldA"
                     /locus_tag="POB34_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HDI5921337.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            1878..1964
                     /gene="uof"
                     /locus_tag="POB34_004505"
     CDS             1878..1964
                     /gene="uof"
                     /locus_tag="POB34_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HDI5921338.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            1957..2403
                     /gene="fur"
                     /locus_tag="POB34_004506"
     CDS             1957..2403
                     /gene="fur"
                     /locus_tag="POB34_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HDI5921339.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(2488..2814)
                     /locus_tag="POB34_004507"
     CDS             complement(2488..2814)
                     /locus_tag="POB34_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921340.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(2864..4270)
                     /gene="chiP"
                     /locus_tag="POB34_004508"
     CDS             complement(2864..4270)
                     /gene="chiP"
                     /locus_tag="POB34_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HDI5921341.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         1189 a         1125 c         1164 g         1215 t
ORIGIN      
        1 ggcgcaggat cttgttgata ccctggatgc actgcagatc gacaaagcaa catttatcgg
       61 tcactccatg ggcggtaaag cggtaatggc acttactgca ctggcccccg atcgcatcga
      121 taaactggtg gcgatcgata tcgcaccggt cgactatcac gtacgccgtc atgatgagat
      181 tttcgcagct atcaacgcgg tcagtgaatc ggacgcacaa actcgccagc aagcagcagc
      241 aataatgcgc cagcatctta atgaagaagg ggtgattcag tttctgttga aatcttttgt
      301 tgacggggag tggcgcttta acgtgccggt attgtgggat cagtatccgc atattgtggg
      361 ttgggagaaa atcccggcat gggatcaccc tgccctgttt atccctggcg gcaattctcc
      421 gtatgttagc gagcagtacc gtgatgattt actggcacaa tttccacagg cacgggcgca
      481 tgtgattgca ggcgcgggtc actgggtcca tgctgaaaaa ccggatgcgg tattacgcgc
      541 tatccgtcgc tatctcaatg attaacgcaa ctgattaaaa acccagcgtc cgcgtcgctt
      601 tccgcgcgtg ggcgttggcg tgactcatcg cgctgatgta ttatggcgcg ctatccatcc
      661 gggcagaggc ctggctgatt gtttcccccg aagtcaccaa gatcatggcc aaagaacaaa
      721 cggaccgtac gacattagat ctgttcgcgc acgagcgtcg accgggacga ccgaaaacta
      781 atccgctttc gcgcgatgaa cagctgcgta ttaataaacg caaccagcta aaacgcgaca
      841 aagtacgtgg ccttaagcgt gtcgaactga agctgaacgc ggaagctgtc gaggcgctga
      901 acgagctggc ggagtcgcgc aatatgagcc gtagcgaact gatcgaagag atgttaatgc
      961 agcaactggc ggcgctgcgt agtcagggca tcgtttaaat ttccactttc atgtagcaca
     1021 gtgtgcagtc ctgctcgttt gctgattccg caagactgcc tgttctgcta tgattgcctt
     1081 tatccgtggg caattttcca cccccatttc aataagtttc aagaggttat ttcactcatg
     1141 gctatcactg gcatcttttt cggcagcgac accggtaata ccgaaaatat cgcaaaaatg
     1201 attcaaaaac agcttggtaa agacgttgcc gatgtccatg acattgcaaa aagcagcaaa
     1261 gaagatctgg aagcttatga cattctgctg ctgggcatcc caacctggta ttatggcgaa
     1321 gcgcagtgtg actgggatga cttcttcccg actctcgaag agattgattt caatggcaaa
     1381 ctggttgcgc tgtttggttg tggtgaccag gaagattacg ccgaatattt ctgcgatgca
     1441 ttgggcacca tccgcgacat cattgaaccg cgcggtgcaa cgatcgttgg tcactggcca
     1501 actgcgggct atcatttcga agcatcaaaa ggtctggctg atgacgacca ctttgtcggt
     1561 ctggctatcg acgaagaccg tcagccggaa ctgaccgctg aacgtgtaga aaaatgggtt
     1621 aaacagattt ctgaagagtt gcatctcgac gaaattctca atgcctgatg tgatgcggcg
     1681 tagactcatg tctacgccgt attaatagat aatgccaatc aaaataattg ctacaaattt
     1741 gtaacttttg ctgttgtacc tgtacaatgt cccggtgttc aagtggcctt gccgttgtaa
     1801 atgtaagctg tgccacgttt ttattaacaa tatttgccag ggacttgtgg ttttcattta
     1861 ggcgtggcaa ttctataatg atacgcatta tctcaagagc aaattctgtc acttcttcta
     1921 atgaagtgaa ccgcttagta acaggacaga ttccgcatga ctgataacaa taccgcccta
     1981 aagaaagctg gcctgaaagt aacgcttcct cgtttaaaaa tcctggaagt tcttcaggag
     2041 ccggacaacc atcacgtcag tgcggaagat ttatacaaac gtctgatcga tatgggtgaa
     2101 gaaattggtc tggctacggt atatcgcgta ctgaaccagt ttgacgacgc tggtatcgtc
     2161 acccgccaca attttgaagg cggtaaatcc gtatttgaac tgacacagca acatcaccac
     2221 gatcacctga tctgcctcga ctgcggcaag gttatcgaat ttagtgatga ttccatcgaa
     2281 gcgcgtcagc gtgaaattgc cgcaaaacat ggcattcgcc tgactaacca cagtctctat
     2341 ctttacggtc actgtgccga aggcgattgc cgcgaagatg aacatgcgca cgaaggcaaa
     2401 taagccagcc tgaatgagaa aaagccaacc tgcgggttgg cttttttatg caagggaaat
     2461 aagaaaagcg agagttacag ctctcactta tttgttatta ctgcgaattt cctgccaaac
     2521 tttatcacaa tcggctttca ccgcctgatc atttccggtt tgcgttgcac gcagacactg
     2581 ctgataatcc agtacgcgga cactttcctg gtcggcaaaa tgctgatgtt gtttctcttt
     2641 cttcagcacg tttaacacgc tctggcaggc ttcaattttt tctggcgaac cttgtgcggt
     2701 gttgatacag gcgctgtatg cctctttcag acggctgtct tcctgtggcg cggttgattg
     2761 cgcacaagcc accagccccg atgccatcat ggcgatgaga atcagttttt tcatagcaaa
     2821 ttcctcaaat gtgcggcagg cacaaaacct gccgcgtcgg gcatcagaag atggtgaatg
     2881 gtgcgattac cataaatttc acgtcacgct catcctggaa gatgttgccg taaccaccac
     2941 cccagcttgg gatgtcggag tggttgtcgt attcggtgaa atgcagtttg aacatcgtgc
     3001 ctttggcgcg accgtcctga atggtgtaga ccgcatccag gctgtatgca gactcttcaa
     3061 tagtccggtt tttgtcgtag tacgcatccg gattgctctg ccaggtcgca ggtttagcat
     3121 cccatgcgta aacgtaggaa gcgccgatgg cgaagcctgg aagattccag tttttcaggt
     3181 catacatcgc accgaagaag actgcttttt cgccgttggc gttgaagtca gaacggttgt
     3241 cccaccagat atccaggcga ccgtttgagg aagcgtaggt tggagtcata cgttgcagga
     3301 agtatccctg ctgaccgtca gccttaaccc aggtgccttc gaggcgcaaa tccactacgt
     3361 cagccgcccg gtaaccaaag gtcaacgcct gcagccaggc ggtgccgtca taaaggtcgt
     3421 tgacgctgcg atcgtcaact ttatcgcggg taccgtagaa ctggtagctg gtggttaacg
     3481 ggctaccggc gatatcaaat ttgtagctgg ctttggcaaa atattgatcg atataccctt
     3541 ccgcctgacc aaaagccgct tccagtacga agttattttt gaagtcgtat ttcgccccaa
     3601 gggagtgcag ataatcaact ttggtggttt tatcgttctg ataaaactca tccatttcca
     3661 gatgccacgg cgctttgtat tcgttggtcc acatgtagga gaaactcaac gcaccggcat
     3721 cgccgtaatc aaaattcgct ccggcttccg caccctgata agtacccggc ataaagctcc
     3781 agtgaggcgc taacagcgtc tgaccggttg gctgaatata acctgccctc gcccaaaccg
     3841 gaccgtattt aaatttggcc gctgctttat acaggcttat accgcttttg tcgccggacc
     3901 agtcttcgtc ataggcttta ttactttttg aaaacgcgat ttcgttcggg tgagagctgt
     3961 cgccgttttc cgccatttca atcgccgtaa acgcggcaat atcaaggccg aacatatcag
     4021 cagcataacc agactgaaaa tcgaggttgg cattccaggt ggagtgagaa aggttggttt
     4081 tgtatttgtc gccgtcggta acatctttac ggtcgcgttc acgctgccag taatagatac
     4141 cgccggttaa ggttgaatcg tcgatgaatc cttcggcgcg agcctccggc attgccataa
     4201 agcccgacat tgctgtaaca ccggcgatag ccagcgccag cgtactacgt ttgccactaa
     4261 acgtacgcat gggttaatcc tctttgacgt ataaattgct gcaccaaagg tgaacagcga
     4321 aaaaaaataa aaatttaaaa agcagcgttg agaaaagtct cgctgactac aggaacagac
     4381 tatttttcgc gacgcgaatt atcaatctct ttctgtaacc agaatgtaag aacatgagca
     4441 accgcacata ttttgatgat ttttgttaca aggcttaagt ttgcggttga aaggcttgca
     4501 gaagaataaa agcgtgaatt tccagcaaga tgaaagcggt tacattttat ttaattatgt
     4561 tttgttattt taaatttatt ttatggataa attgcgaagc aggattaatt cgcgtagaaa
     4621 agtagaaaaa aacgccacag agcgcggcgt tttggattgt ttgctgtttc ttgccggatg
     4681 cggcgtgaac gcc
//
LOCUS       DAMZET010000250         4645 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.250, whole genome shotgun sequence.
ACCESSION   DAMZET010000250 DAMZET010000000
VERSION     DAMZET010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.250"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..219)
                     /locus_tag="POB34_004509"
     CDS             complement(<1..219)
                     /locus_tag="POB34_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDI5921342.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNW"
     gene            complement(216..1286)
                     /gene="ugpC"
                     /locus_tag="POB34_004510"
     CDS             complement(216..1286)
                     /gene="ugpC"
                     /locus_tag="POB34_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HDI5921343.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            complement(1288..2133)
                     /gene="ugpE"
                     /locus_tag="POB34_004511"
     CDS             complement(1288..2133)
                     /gene="ugpE"
                     /locus_tag="POB34_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HDI5921344.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLQMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(2130..3017)
                     /gene="ugpA"
                     /locus_tag="POB34_004512"
     CDS             complement(2130..3017)
                     /gene="ugpA"
                     /locus_tag="POB34_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HDI5921345.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYIDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(3115..4431)
                     /gene="ugpB"
                     /locus_tag="POB34_004513"
     CDS             complement(3115..4431)
                     /gene="ugpB"
                     /locus_tag="POB34_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HDI5921346.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEALESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
BASE COUNT         1069 a         1321 c         1241 g         1014 t
ORIGIN      
        1 ccagttcagt tcacccgcga cgccccagcc gttgctggtg cgttcgagat tgtcgtcatg
       61 gagcaggaag atctcgccat ctttcgataa cttcgcgtca aattcgatca tcttatgacc
      121 gtattttgcc ccgacgtcga ttgccgccag ggtgttttcc ggggccagct taccgccgcc
      181 acgatgagcg acgatgcggg gataaggcca gttactcata ctcgttgtcc tgtttcacca
      241 tcaaaaagat gcagctgatt ttccgccaga tgcagccaca gcgtgctgcc tgccgtcggg
      301 cgctcctgat gcgccagtcg caccaccagc ttctgctcgc cccagcgtcc gtgcgccagg
      361 ttatctgcgc cgaggatctc cagcgtgtcc atcaccagcg gcacaccacc ttctgcctgc
      421 gagcttagcg ctatatgttc cgggcgaatg ccgagagtca ttttacgccc ggcatactga
      481 cggtagccac cgtttagcgg tagcacgata ccgccgtcca gttcgaaatg cgtgccttca
      541 ttattcacgc ggcctgtcag caggttcatc gccgggctgc cgataaaact cgccacaaac
      601 aggctggcgg gcttttcgta gacttccacc ggcgtgccaa tctgttcggc aacgccgccg
      661 ttcatcacca ttactcgctg ggcgagggtc atcgcttcaa cctgatcgtg agtaacgtag
      721 agtgaagtcg ttttcaggcg acggtgcagc tgttgcagct caaggcgcat ctgcacgcgc
      781 agcttggcat cgaggttaga gagcggctca tcaaacagga acaccgccgg atcgcgcaca
      841 atcgcgcgac ccatcgccac acgctgacgc tgaccgccgg atagctcgcg cgggcggcgc
      901 ttaagcaggc catccagctc cagaatgcgc gccgcttctt taacgcgctc ggctatttgc
      961 tgcttgccca tgccgcgaat tttcagcccc cacgccatgt tttcttcgac gctcatatgc
     1021 ggataaagcg cgtagttctg gaacaccatc gcaatcccgc gatctttcgg ctccatttcg
     1081 gtcacgcgct ggtcgttgat ccagatatcg ccttccgtca cccgctccag cccggcaacc
     1141 atgcgcagca gcgtcgattt tccgcagcca gacggcccga ccatcacgat aaattcgcca
     1201 tccgccacat caagggtcag tggtttaatc acctgggttt tgccatccca gcttttggtt
     1261 actgcctgta atttcagtcc tgccatctta tttctcacta tcgaccaggc cgcgcacgaa
     1321 ggcacgctgc atcactaaaa caatcaccac cggagggata agcgttaaca acatcgccgc
     1381 cattaccgag ttccattccg tggtgccttc gccagtggcg atcatccctt tgatccctgc
     1441 cacggtggtg ccgagatcaa cgtcggtaat aatcaacaac ggccacaaat actgattcca
     1501 gccgtagata aaggtgatca caaacagcgc cgccagatta gttttggaga gcggaaaaac
     1561 gatgtcgcaa aagaagcgca ttggcgatgc gccgtcgatc cgcgcggctt ccaccagctc
     1621 atccggcagc gtcataaaga actggcggaa caggaaagta gcggtcgccg aggccatcag
     1681 cggcagcgtt aaaccggcgt agctgtcgag catctgcagg ttggcgatga cttccaccgt
     1741 cgggaagata cgtacttcaa ccggcagcat cagggtgata aaaatcatcc agaagaagag
     1801 gttacgtagc ggaaaacgaa accagacaat ggcaaatgcc gagagcatcg agacggtaat
     1861 tttgccgagc gtaatgctga acgccatcac aaagctgtta agcaacatcc gccagaacgg
     1921 cgcgctattc gtgcctaccc cgtttaccca gatgttgtgg atgttttcca gcagatgcgt
     1981 gccggggatt agcgtcatcg gcgcggcgta gacggcctgt ttatccagcg ttgccgctac
     2041 aaacgccacg tacagcggga agaggatcac cgcgatcccg aggatcagca tggtatggct
     2101 gaatatcgtc agccacggac ggttctcaat cattggtaac gcaccttgct ttcaacatag
     2161 cggaactgca ccaccgtcag cacgatgacg aggaacatca acaccaccga ctgcgcggca
     2221 gacgaagcca gatccagtcc ggtaaagcct tcgcggtaga tcttataaat cagcgtcgtg
     2281 gtggcctgaa ccggcccgcc ggacgtggcg gcgtcgatca ccgggaaggt gtcgaagaag
     2341 gcgtacacca ggttcaccac cagcaggaag aaactcaccg gagcgataag cggcagagca
     2401 atcttaaaga agcggcgaat cggccctgcg ccgtcaatag ctgcggcttc gatcaacgaa
     2461 cggggaatgg attgcagcgc ggcatagaag aacagaaagt tgtagctgat ttgcttccac
     2521 accgaggcaa acaccaccag aaacatcgcc tgaccgctgt tttgcgcgtg gttccagtcg
     2581 tagccgaact cagcgagaaa atgggtgatc agcccgcgac cggggttaaa caggaagatc
     2641 cacaatacgg cggcaacggc gggagccacg gcatacggca gcagcattaa ggtttgatag
     2701 aaacggctgc cgcgcacgat gtactccacc agcgccgcaa agaacagcga taccagcaaa
     2761 ccgctgacgg tgacaaaggt gctgaatttt atcgtcgtcc agaaggagtc gatatagtag
     2821 ctgtcatgaa acagcgcgac gaagttatcc aggccgacaa actggctgga gaaaccaaat
     2881 ggatcgacgc tttgtagcga gtaccacaac gcttcgcccg caggccagat aaaaaagata
     2941 acggtgatga tgagctgcgg cgcgaccagc agataaggca gccagcgcga gcggaacacc
     3001 ggacgggatg atgacattac ggttaattcc tgaactgtgc cggatgcgct tcgcttatcc
     3061 ggcctacacg cgatgccagg tcagataagc gaaacgcatc cgacattaca ctgattaaga
     3121 cttcgtcgat ttctcaaagc ggcgcagtaa ctggttccca cgctcaacgg cggtatccag
     3181 tgcctgctgc ggtgtcttct taccggtcca cacgctctcc agcgcttcat ccacaatcac
     3241 gcggatctgc ggcatgttgc ccagacgcag ccctttggtg aacggcaacg gcggcttgtt
     3301 cagcatctga cgtgtcgcgg tatccgcccc tgggtttttc tcataaaagc cctgctcacg
     3361 ggtcaggtca tacgccgctt tggtaattgg cagataaccg gttttctgat gccactcagc
     3421 agcgttttct ggcttcgcga ggaaatcgag gaacttcgcc acaccggtat acgtttcttt
     3481 atctttaccc tgcatcaccc acaggctggc tccgccgata atggcgtttt gtggcgcatc
     3541 tttcgcatcg gcgtcgtaag gcatcatgcc tacgccgtag ttgaatttgg cgtactcgcg
     3601 aatgttggca agagaaccgg aagaggcggt ggtcatcgcg caatcaccgt tatagaactt
     3661 ctcggtggat tcatccttac gcccgacgta gctgaaatcg cccttcttgt tcatctcttc
     3721 gagcatggcg atgtgtttca cctgctccgg cttgttgaac tccagcaccg cgtccgtgcc
     3781 gtcaaagccg ttgtttttgc tggcaaacgg cagaccgttc caggcgctaa agttttccag
     3841 ttggatccag ccctgccagc cgctggcgta gccgcacttc ataccggagg ctttcagttt
     3901 tgcggaatag tccgccagat cctgccaggt tttcggcggc tgttccgggt ctaaccccgc
     3961 tttcttgaag gcgtctttgt tgtaatagag aacgggagtc gagctgttga atggctggga
     4021 gagtaagtgg cccgttttgc tgtcggagta gtaaccggaa accgtcggca caaactgcga
     4081 ctcatcgaat tgaatccccg cctctttaaa cacgtcatac accggtttaa tggctttcga
     4141 cgccatcatg gtggcggtgc caacttcata aacctgcaaa atagccggtg cgttgccggt
     4201 acgaaatgcg gcaatccccg cgcttaaatt ctgttcgtag ttgcctttat aggtcggtac
     4261 aattttgtaa tccgggtttt cggcgttaaa acgttgggcc agagaatcca cctctttacc
     4321 cagttcccct tccatagaat gccagaacgg aatggtcgtc actgcctgtg cattccccat
     4381 taacgccagt ccgagcgcca gtgctgaagc tgtataatgt aacggtttca tcgtttatct
     4441 ctcttgttgt accgaatgcg cgaattcacg cgttttatgc tcgcggggta acatgacatg
     4501 ctcgaattac agaaaaataa cttttttgtt acatttgtaa gatagtaagg tgtcagaaag
     4561 atgacaaggc ggtgacggcg tgggtgaggg aaaatgggag atggggcacg gataagcgga
     4621 aaaatagagg gtctgaatca aactc
//
LOCUS       DAMZET010000251         4644 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.251, whole genome shotgun sequence.
ACCESSION   DAMZET010000251 DAMZET010000000
VERSION     DAMZET010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.251"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..654
                     /gene="mdtL"
                     /locus_tag="POB34_004514"
     CDS             <1..654
                     /gene="mdtL"
                     /locus_tag="POB34_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HDI5921347.1"
                     /translation="LFILKETRPAAPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            629..1588
                     /gene="yidZ"
                     /locus_tag="POB34_004515"
     CDS             629..1588
                     /gene="yidZ"
                     /locus_tag="POB34_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HDI5921348.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKQIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWSLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            1745..2494
                     /locus_tag="POB34_004516"
     CDS             1745..2494
                     /locus_tag="POB34_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDI5921349.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPLHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            2516..3082
                     /gene="yieF"
                     /locus_tag="POB34_004517"
                     /gene_synonym="chrR"
     CDS             2516..3082
                     /gene="yieF"
                     /locus_tag="POB34_004517"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HDI5921350.1"
                     /translation="MSEKLKVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(3136..4473)
                     /gene="adeP"
                     /locus_tag="POB34_004518"
     CDS             complement(3136..4473)
                     /gene="adeP"
                     /locus_tag="POB34_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HDI5921351.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALMFILKIVFIDAH"
BASE COUNT         1178 a         1210 c         1193 g         1063 t
ORIGIN      
        1 ttgtttattt taaaagaaac gcgccctgcg gcccccgcag cttcggacaa accacgagaa
       61 aatagcgagt cgctgcttaa ccgttttttc ctcagccgtg ttgttatcac caccctcagc
      121 gtttcggtga tcctcacttt cgtcaacacg tcaccggtat tgctgatgga aatcatgggg
      181 tttgagcgcg gtgaatatgc caccattatg gcgctgaccg ctggcgtcag catgaccgtt
      241 tcattctcca cgccatttgc gctgggaatt tttaagccac gtacgttgat gatcacctcg
      301 caggtgttat tcctggcggc ggggatcacc cttgccgttt caccttccca tgcggtttct
      361 ctgtttggta tcacactgat ttgcgccggt ttctcggtcg gttttggcgt agcgatgagt
      421 caggcgttag ggccgttttc attacgcgcg ggcgtagcca gctcgacctt aggtattgcg
      481 caggtttgcg gttcgtcact gtggatttgg ctggcggcgg tggttggtat cggcgcatgg
      541 aatatgctga tcgggattct gattgcctgt agcatagtga gcctgttgct gattatgttc
      601 gtcgcgcctg gacgccccgt tgccgctcat gaagaaatcc atcaccacgc ttgatctcaa
      661 tttgctgctc tgtctgcaac tgctgatgca ggagcgcagc gtgaccaaag cggcgaagcg
      721 gatgaacgta accccttcgg cggtgagtaa gtcgctggca aagttaagag catggtttga
      781 cgacccgctc tttgtgaact caccgctggg gctgtcgccc acaccgctaa tggtcagcat
      841 ggagcaaaat ctggcggagt ggatgcaaat gagcaacctg ctgctggata aaccgcacca
      901 ccaaacgccg cgcggcctga agtttgagct ggcggcggaa tcaccgctga tgatgatcat
      961 gcttaatgcg ctgtcgaaac agatctacca acgttacccg caggcgacca tcaaattacg
     1021 taactgggat tacgattcct tagatgccat tactcgtggt gaagtggata ttggtttttc
     1081 cggtcgcgaa agccatcctc gctcgcggga gctgttaagc tcgctaccgt tagccattga
     1141 ttatgaagtg ctgtttagtg atgtgccctg cgtctggtta cgcaaagatc atccggcact
     1201 gcatgaaacg tggagtctgg acactttttt gcgttatccg catatcagca tttgctggga
     1261 acagagcgat acctgggcgc tggacaatgt gttacaggag ctggggcgcg aacgcacgat
     1321 tgctatgagc ctgccggaat tcgagcagtc actgtttatg gcagcgcagc ccgacaatct
     1381 gctactggcg accgcgccgc gctactgtca gtactacaat caactccacc aactgccgtt
     1441 ggttgcgctt cctctcccgt ttgacgaaag ccagcaaaaa aagctggaag tcccttttac
     1501 cctgctttgg cataaacgga acagccataa tccgaagatc gtctggttac gggaaaccat
     1561 taaaaatctt tacgcgtcga tggcataacc gaatcgtatg aaacgcgacc ctatttcaca
     1621 ataaaatgta aaaattttgt aataaacttg tctgaatcga acttttagcc gctttagtct
     1681 gtccatcatt ccagtaaatg attactcttg tattcataat ggaccattaa gcatggagcg
     1741 aaaaatggcg actcactttg cccgagggat tttaacggaa ggacatctga tttctgttcg
     1801 tctcccctcc cagtgtcatc aagaagcccg aaacattccc cttcatcgtc aaagccgttt
     1861 tctggcgtcc agaggtttac tcgcagaact gatgttcatg ctgtatggca ttggcgaatt
     1921 gccggaaatt gtcaccctgc cgaaaggtaa accggttttc agcgataaaa atttgccttc
     1981 gttttctatt tcctatgccg ggaatatggt tggcgtggcg ttaacaaccg aaggtgaatg
     2041 tggcctcgat atggaactac agcgtgcgac gcgcgggttt catagcccac acgcgcccga
     2101 taaccacacc ttttcaagca atgaatcgct atggatcagt aaacaaaacg atcctaacga
     2161 agcgcgggcg cagctcatca cgctgcgccg aagcgtgcta aaactaaccg gtgatgtttt
     2221 gaatgacgat ccgcgcgatc tgcagctgct gcccattgcc ggacgcctga aatgtgctca
     2281 tgtaaatcat gtagaagcgt tatgcgacgc ggaagacgtg ctggtgtggt ccgtggcggt
     2341 cacgcccacg attgaaaagc tcagtgtctg ggagttagat ggcaaacacg gctggaaaag
     2401 cctgccggat attcacagcc gcgccaacaa tcctaccagc cggatgatgc gttttgccca
     2461 gctctctacc gtgaaggctt tttcgccaaa ttgatagaca accacaggag tcatcatgtc
     2521 tgaaaaattg aaggtggtta cgttactggg gagcctgcgc aaaggctcat ttaatggcat
     2581 ggttgcacgt accctgccga aaattgctcc ggcgagcatg gaagtcaatg cgttaccttc
     2641 cattgccgac attcccttgt atgacgctga cgtacagcag gaagaaggtt ttccagcaac
     2701 ggtagaagct ctggcggaac agatccgtca ggctgacggt gtggtgattg tcacgccgga
     2761 atataactac tcggttccgg gtgggctgaa aaatgccatc gactggcttt cccgcctgcc
     2821 ggatcaaccg ctggccggta aaccggtgtt gattcagacc agctcaatgg gcgtgattgg
     2881 cggcgcgcgc tgtcagtatc acctgcgcca gattctggtt ttcctcgatg caatggtgat
     2941 gaacaagccg gaatttatgg gcggcgtgat tcagaacaaa gttgatccgc aaaccggaga
     3001 agtgattgat cagggtacgc tggaccacct gaccgggcaa ttgaccgcat ttggtgagtt
     3061 tattcagcga gttaagatct aaataaaaaa cccgccagta atcatgcatg gcgggttttt
     3121 aatgcgctat cgattttaat gagcgtcgat aaatacaatc ttcaggataa acatcagcgc
     3181 aacgatgatt acgcacgggc taaggtcacg cagacgcccg gtaccaatct tcatcacgca
     3241 gtaggagata aagcccagcg caataccttc ggtaatcgaa aagctgaacg gcatcatcac
     3301 ggcggtaata aacgccggaa cagattcagt aagatcctgc cagttcacgc gagcaagact
     3361 tgaggtcatc aacacgccaa cgtagatcaa cgcgccagct gcagcgtagc ctggcaccat
     3421 tcccgccagc ggcgacagaa agataaccag caggaacagc agaccaacaa ccactgccgt
     3481 cagaccggta cgaccgccaa ccgatacgcc ggaagaggac tcaatataag ccgtaacgga
     3541 agaagtaccg ataaacgaac cggtcacgga agagatactg tcgacataca gcgcctgctt
     3601 catgcgcggg aatttaccct tctcatccgc caggcctgct ttatcggtca cgccaatcag
     3661 cgtaccggag gagtcaaaca ggttgaccag catgaaagag aaaatcaccc ctgccagccc
     3721 gaggttaaac gacccggcta aatctacatg gcccacgaca gtcattacgc tcggcggcgc
     3781 agaaacgatg ccattgtagt ggacatcacc cagcatccag cccagcagcg ttgtcaccac
     3841 gatggaaacc agcaccgccg cgtgaatgtt gcgcgaggcc agaatagcga tgatgaagaa
     3901 gccgaggatc cccagcagca cgctgtgaga agtcaggtta ccgatgctga ccagcgtttc
     3961 cgggttagcg acaatcacac ctgcattctt cagtcccatc ataccaatga acagaccgat
     4021 accgctggtg atgcccacac gcagacttac cggaatgtta gcaatcatcc agtaacgaac
     4081 gcggaaaatc gtcagtaaca gcagaccaat cgcgccccag aagattgcgc ccatcccgac
     4141 ttgccacggc aagcccatag cctgtacaac gacaaaagcg aagaatgcat tcaggcccat
     4201 agcgggtgcc agtgcaactg gcaggttagc aaacagtccc atcataatac tgccgaatgc
     4261 agcaatcaga caggtagtaa cgaagacggc gctggtatcc atgccagcaa cgccaagaat
     4321 ttgcgggtta acaaaaacga tgtaaaccat cgtcaggaag gtggtaaaac cggcgatcac
     4381 ttcggtccgt gccgtcgtgc catgttcgcg cagtttaaac acgcgttcca gcatcccctg
     4441 gccagaagcc tgggttgtgt gttgttgact cattatctat ttccgaacaa ggagggaaaa
     4501 tccgtcgcta tcgtatacca aaatgagaca atatggacgg ctgaagataa ctttttttca
     4561 ttgtatttgc ttatacggca acgattgcgt catgcgaatt ggcatgacga aaacgttaaa
     4621 ctaacgataa aggaaagaca tgtc
//
LOCUS       DAMZET010000252         4601 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.252, whole genome shotgun sequence.
ACCESSION   DAMZET010000252 DAMZET010000000
VERSION     DAMZET010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.252"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..724
                     /gene="kdpA"
                     /locus_tag="POB34_004519"
     CDS             <1..724
                     /gene="kdpA"
                     /locus_tag="POB34_004519"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit A"
                     /protein_id="HDI5921352.1"
                     /translation="INMEGKESRFGVLVSSLFAVVTTAASCGAVIAMHDSFTALGGMV
                     PMWLMQIGEVVFGGVGSGLYGMMLFVLLAVFIAGLMIGRTPEYLGKKIDVREMKLTAL
                     AILVTPTLVLMGAALAMMTDAGRSAMLNPGPHGFSEVLYAVSSAANNNGSAFAGLSAN
                     SPFWNCLLAFCMFVGRFGVIIPVMAIAGSLVSKKSQPASSGTLPTHGPLFVGLLIGTV
                     LLVGALTFIPALALGPVAEYLS"
     gene            747..2795
                     /gene="kdpB"
                     /locus_tag="POB34_004520"
     CDS             747..2795
                     /gene="kdpB"
                     /locus_tag="POB34_004520"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDI5921353.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMCLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            2804..3376
                     /gene="kdpC"
                     /locus_tag="POB34_004521"
     CDS             2804..3376
                     /gene="kdpC"
                     /locus_tag="POB34_004521"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDI5921354.1"
                     /translation="MSGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVINIVELNLALDKLDE"
     gene            3369..>4601
                     /locus_tag="POB34_004522"
     CDS             3369..>4601
                     /locus_tag="POB34_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="sensory histidine kinase in two-component
                     regulatory system with KdpE; signal sensing protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
                     /protein_id="HDI5921355.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRTQGLDIVVGVVETHGRKDTAAMLEELAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAV"
BASE COUNT          943 a         1222 c         1400 g         1036 t
ORIGIN      
        1 catcaatatg gaaggtaaag agagccgttt cggcgtgctg gtcagtagcc tgtttgcggt
       61 cgtgactacg gcggcttcct gtggcgcggt gattgcgatg catgattcgt ttaccgctct
      121 cggtggcatg gtgccgatgt ggctgatgca aattggtgaa gtggtgttcg gcggtgtcgg
      181 ttctggtctt tacggcatga tgctgtttgt cctgctggcg gtgtttattg ccgggctgat
      241 gattggtcgt acaccggaat atctgggtaa aaaaatcgac gtacgcgaga tgaaactgac
      301 tgcactggcg attctggtta ccccgacgct ggtgctgatg ggcgcggcgc tggcaatgat
      361 gaccgacgcc ggacgtagcg ccatgctcaa ccctggcccg catggtttta gcgaagtgct
      421 gtacgccgtg tcatccgccg ctaacaacaa cggcagcgcc tttgccggat taagcgccaa
      481 ctctccgttc tggaactgtt tactggcgtt ctgcatgttt gtcggtcgct tcggggtgat
      541 tatcccggtg atggcaattg ccggttcgct ggtgagtaaa aagagccaac ccgccagctc
      601 cggcacgctg ccaacgcacg gcccgctgtt tgttggcctg ttaatcggca ccgtgttgct
      661 ggttggcgca ctgaccttta tccctgccct ggcgcttggt ccagtggcgg aatatctctc
      721 ctgatgatat tgagtgagca ctgaatatga gtcgtaaaca actggcgcta ttcgaaccaa
      781 cacttgtcgt tcaggcgctg aaagaagcgg tgaaaaaatt aaacccgcag gcgcaatggc
      841 gcaatccggt gatgtttatc gtctggatcg gcagtctgct gaccacctgt attagcatcg
      901 cgatggcaag cggtgcgatg cctggcaatg cgctgtttag cgcggcaatt agcggctggc
      961 tgtgggtcac cgtactgttc gctaatttcg ccgaggcgct ggcagaaggc cgcagtaaag
     1021 cgcaggccaa cagtcttaaa ggggtgaaaa aaactgcctt tgcccgcaag ctgcgtgagc
     1081 cgaaatatgg cgctgcggcg gacaaagttc ctgccgatca actgcgtaaa ggtgacatcg
     1141 tgctggtaga agctggcgat attatcccct gcgatggtga agtcattgaa ggcggcgcat
     1201 cggtcgatga aagcgccatc accggggaat cggcaccggt gatccgtgaa tccggcggcg
     1261 attttgcctc cgtcaccggc ggcacgcgta ttctttctga ctggctggtg attgagtgta
     1321 gcgttaaccc cggtgagaca tttctggatc ggatgatcgc gatggtggaa ggcgcacagc
     1381 gacgcaaaac accgaacgag attgccctga ctatcctgct gattgctctg accatcgtct
     1441 ttttactggc aaccgccacg ctgtggccgt tttccgcgtg gggcggtaat gcagtcagcg
     1501 taacggtact ggtggcgctg ctggtctgtc tgatcccaac cactattggc ggcctgttgt
     1561 cagcgatcgg cgtcgccggg atgagccgga tgctaggcgc gaatgtgatt gccaccagcg
     1621 gacgtgcagt tgaagcggca ggtgacgttg acgttctgct actggataaa accggcacca
     1681 tcacactcgg taaccgtcag gcgtcggagt ttatccccgc gcagggcgtg gatgaaaaaa
     1741 cgctggctga cgccgcacaa ctggcttcgc tggctgatga aacgccggaa ggccgcagta
     1801 ttgtgatcct cgccaagcag cgttttaacc tgcgcgagcg cgatgtgcag tcgctccatg
     1861 ccacctttgt accgtttact gcgcaaagcc ggatgagcgg gatcaacatc gacaaccgca
     1921 tgatccgtaa aggttctgtc gatgccattc gtcgccatgt tgaggctaac ggtggtcact
     1981 tccctaccga tgttgatcaa aaagtcgatc aggttgcgcg tcagggagcc acgccgctgg
     2041 tggtggtgga aggttctcgt gtgctgggcg ttattgcgct gaaagatatc gtcaaaggcg
     2101 gtattaaaga gcgcttcgcc cagctgcgca aaatgggcat taaaacggtg atgattaccg
     2161 gcgataaccg tctgactgcc gccgcgattg ctgcggaagc gggtgtcgat gattttctcg
     2221 ccgaagcgac accggaggcc aagctggcat tgatccgtca gtatcaggcg gaaggtcgtt
     2281 tggtagcgat gaccggcgac ggcaccaacg atgctccggc gctggcgcag gcagatgtcg
     2341 cggtggcgat gaactccggc acccaggcgg cgaaagaggc gggcaatatg gtcgatctcg
     2401 actctaaccc gaccaagttg atcgaggtgg tgcacattgg caaacagatg ctgatgaccc
     2461 gtggctcgct gaccaccttc agcattgcca acgatgtggc gaaatacttc gccattattc
     2521 cggcggcatt cgcggcaacg tatccgcagt taaatgcgct gaacatcatg tgcctgcatt
     2581 cgcccgactc cgcaatcctc agtgcggtga ttttcaacgc cttgattatc gtctttttga
     2641 ttcccctggc gttaaaaggc gtgagttata aaccgcttac cgcttctgcc atgttgcgcc
     2701 gtaacttatg gatttacggt ctgggtgggc tgctggtgcc gtttatcggt atcaaagtca
     2761 ttgatttact gctgaccgtt tgcggtctgg tgtgaggttt accatgagtg gattacgtcc
     2821 ggcattatca acatttatct ttctgttatt gattactggc ggcgtttacc cgctgctgac
     2881 caccgtactg gggcaatggt ggtttccctg gcaggccaat ggttcgttga ttcgtgaagg
     2941 tgatacggtg cgcggttcgg cattaatcgg gcagaatttt accggcaacg gctattttca
     3001 tggtcgcccg tcggcaacgg cagaaatgcc ctataatcca caggcttctg gcgggagcaa
     3061 tctggcggtc agtaaccctg agctggataa actaatagcc gcacgcgttg ctgcattacg
     3121 ggccgctaac ccggatgcca gcgcgagcgt tccggttgaa ctggtgacgg catcggcaag
     3181 cgggctggac aataatatca ccccgcaagc ggcggcctgg caaatcccac gcgtggcgaa
     3241 agcgcgtaat ctcagcgttg aacagctcac gcaactgatc gcaaaataca gccaacaacc
     3301 gctggtgaaa tatatcggcc agccggttat caacattgtt gaactcaatc tggcgctgga
     3361 taaacttgat gaataacgaa cccttacgtc ccgaccccga tcgtctgctg gaacaaactg
     3421 ccgcgccgca tcgggggaag ctgaaagttt tcttcggtgc ctgtgcaggc gtcgggaaga
     3481 cctgggcgat gctggcagaa gcacagcgac tgcggacgca agggctggat attgtggttg
     3541 gcgtggtaga aacccacggg cggaaagata ccgccgccat gctggaagag ctggctgttc
     3601 tgccgccaaa acgcctggcg taccgtgggc ggcatatcag cgagtttgat ctcgatgccg
     3661 ccctcgcccg ccgtccggcg ctgatcttaa tggacgaact ggcgcacagt aatgcgcctg
     3721 gttctcgtca tcccaaacgc tggcaggata ttgaagagct gctggaagca ggcattgatg
     3781 tttttactac cgtcaacgtt caacatctgg aaagcctgaa tgatgtggtc agcggcgtca
     3841 ccggaattca ggtacgggaa accgtgcccg atcctttttt cgatgccgcc gacgacgtgg
     3901 ttctggtgga cttgcccccg gacgatctgc gccagcggct gaaagaaggc aaagtctata
     3961 ttgccgggca ggcggagcgc gccattgaac attttttccg caaaggcaat ctgatcgccc
     4021 tgcgcgaact ggcactgcgc cgtactgccg atcgcgtaga tgagcaaatg cgcgcctggc
     4081 gggggcatcc tggcgaagag aaagtgtggc acacgcgcga cgcgatcctt ttatgcatcg
     4141 ggcataacac cggcagtgag aaactggtcc gcgcggcggc gcggctggcg tcgcggctgg
     4201 gtagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta ccggagaaaa
     4261 aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg gagacggcaa
     4321 cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa cataatctcg
     4381 gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa acgtttgctg
     4441 accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt gatgaaccac
     4501 ccgcccgcac gattaacaac gcgccggata gccgctcttt taaagacaag tggcgggtac
     4561 agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt t
//
LOCUS       DAMZET010000253         4583 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.253, whole genome shotgun sequence.
ACCESSION   DAMZET010000253 DAMZET010000000
VERSION     DAMZET010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.253"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..814)
                     /locus_tag="POB34_004523"
     CDS             complement(<1..814)
                     /locus_tag="POB34_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDI5921356.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRF"
     gene            1061..1687
                     /gene="gstB"
                     /locus_tag="POB34_004524"
     CDS             1061..1687
                     /gene="gstB"
                     /locus_tag="POB34_004524"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HDI5921357.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1684..2799)
                     /gene="yliI"
                     /locus_tag="POB34_004525"
     CDS             complement(1684..2799)
                     /gene="yliI"
                     /locus_tag="POB34_004525"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HDI5921358.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(2910..3293)
                     /gene="bssR"
                     /locus_tag="POB34_004526"
     CDS             complement(2910..3293)
                     /gene="bssR"
                     /locus_tag="POB34_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HDI5921359.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3506..>4583
                     /gene="rimO"
                     /locus_tag="POB34_004527"
     CDS             3506..>4583
                     /gene="rimO"
                     /locus_tag="POB34_004527"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDI5921360.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEER"
BASE COUNT         1142 a         1201 c         1110 g         1130 t
ORIGIN      
        1 taaaacggat acggtcagtt ttcgccccca gcactacgga gattaaacgc atatcgccct
       61 gggtagccga agcaaccaga ttatatcccg cgcctgccgt ggttcctgtc ttcatgccat
      121 caacattcag attgctgctc cacagcagac ggttacggtt aggctgacga attttgttga
      181 aggtgaactc tttctcttta tgaatggcgt actcttccgg cacatcgtgg atcaatgctt
      241 tacccagcaa tgccatatcg cgcgcggtac tgaactgccc cggcgcatcc agaccgtgca
      301 ccgtctggaa ggtagtgttg gtcagaccca gttttttggc ataaccattc atcaaaccaa
      361 tgaatgactc ctggctcccg gcaacgtaat cggccagcgc aatacaggcg tcattaccgg
      421 actggataat cacacctttg ttcaagtctg ccaccgaaac ctgatcgccc ggtttgagga
      481 acattaccga tgaaccacgc agtgccggat ttcccgtcgc ccaggcatct ttaccgaccg
      541 tcaccatatc ggtgagttta atcttatcgg ccttaagcgc ctgcccaacc acatagctgg
      601 tcatgatttt agtcaggctc gcgggatcca gtttctcatc cgcgttgcct tctgccagca
      661 ctttaccgct ggcgtaatcc attaaaatcc atgcacgcgc atccacgctc ggcgcttcaa
      721 cggtttgttc cgccgcgaat gccgttgggg caaaaaggaa taaaaatgca gaacccgctg
      781 caagaccacg aaggagagag gagtattgcg tcataaatgc cacccgagta tccattcaaa
      841 aaaaatacgt tatatcgccg tatgataagt tcggtgatta ataacgcaca cacaaactta
      901 aagaaaccgt aagttggtaa agtttttaaa gtttatgcaa tatcctgccg cttcgctgct
      961 tctggtgtca tttttttgcc agctgttgct taaaagttaa ctttacttca ccatcggagt
     1021 cctgttcagc ctgtggctgc ctgtcggaaa aggggtgagt atgattacgc tgtggggtcg
     1081 gaataattca accaacgtaa aaaaagtatt gctgacgctc gaagaactgg aactacctta
     1141 tgagcaaatt ctcgcgggcc gtgagtttgg gataaatcac gatgctgatt ttctggcgat
     1201 gaaccctaac gggctggtgc cgttgttgcg tgacgacgaa agtgatctca ttctttggga
     1261 atcaaacgcc attgtccgct atctggcggc acagtacggg caaaaacgtc tgtggatcga
     1321 ctcaccggca cgtcgtgcgg aagcggaaaa atggatggac tgggcaaacc agacgctcag
     1381 taatgctcat cgcgggatcc tgatgggatt agtcagaaca ccaccggaag agcgcgatca
     1441 ggccgccatt gatgccagtt gcaaagagtg cgacgccctg tttgccctgc tcgatgcgga
     1501 actggcaaaa gtaaaatggt tctctggcga cgagtttggt gtgggcgata tcgctatcgc
     1561 accgttcatc tacaatttgt ttaacgttgg cctgacctgg acaccgcgtc caaatctgca
     1621 acgctggtat cagcaactca ctgaacgccc cgcagtgcgc aaagtggtga tgattcccgt
     1681 tagctaattg cgtgggctaa ctttaagtaa ttccccactg gactcgtcgg tgagaacgta
     1741 taaataaccg tcgggtccag tgcgaacatc acgaattcgc tgccctctgt ccgttaaaat
     1801 acggccatct tctgtcactt tgtcgccgtt gacgctcatc acaatgacat ctttatcttt
     1861 cagcgcgcca ataaataatt tttgctgcca ctgggggaat ttatcgctgt tatagaaggc
     1921 catgccgctc acagcgggcg aatctttcca gtaaaaaaca ggttgctcgg tcccggcgac
     1981 gatctcccct ttcgcttccg gtatcttaaa gcctgaatag ttgattcccc aggttgccag
     2041 cggccagccg tagtttttgc ctttttgcgg gatattaatt tcatcaccac cgcgcgggcc
     2101 atgttcattc agccacagtg cattactcca cggattcatc gccattcctt gcggattacg
     2161 aatgccataa gaccagatct cggcgcgcac accggattcc tttataaaag gattatcatc
     2221 cgggatttcg ccctggtcgg tcagacgcac cagtttgccc tgtaatttat ccagatcctg
     2281 cgccgtcggg cgctgattgt tttcgcccag agcaataaaa agataacctt taccgtcgaa
     2341 taccagccgc ccgccaaaat ggttgccggt agacagtttt ggcatctggc ggaagacggt
     2401 gcggaagtcg gtcacttttg agagatcatc acttaagcgg ccataaccca cagcagttcc
     2461 ggctttgcca tcatcgccaa cttcggaata acttaaccag atgcggcgag actgagcaaa
     2521 atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg tgcgcccaaa cgtccggaac
     2581 tccggaaagc ggcgcagata atccttttcc tgcttgccag tgacgcaact cgccgccgcg
     2641 cagagtgatt aacataccgt gattatcggg taaaaaggcc agtgcccagg gatggtcgag
     2701 tttgtcttgc agtacttcga catttaccgt tgcaggagcc gcccagagag cggaagaaag
     2761 acaaataagg ggcacaagga aaaaggattg tcgatgcata acgcctccgg attgactctg
     2821 gcttaaagcg tagtcagtgg aggagataac aaattcattt ttacaaaaac ttaaacatga
     2881 agggggagac gctttctccc ccttagtttt caggccttct caagcatggc gtgcttctgc
     2941 aggctctgga tactcagcgt taagctcatc agacaatttt caagcttatc ggcgttgacg
     3001 gtaataacag tcgggcaatc atggtgccca ctcatcaaac atactgcggc tgtcgctaat
     3061 gcttcttcag catgatgaag agcactccac tcttcctgat ccagatgaag attcaaccgc
     3121 agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt tatctcgtag atgattgctt
     3181 tcgctgacgg acatgtatcc ttttgccttc ctcagttgca cgtatgcagc gaggtcaacg
     3241 cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc tgtcaacgaa catacttcga
     3301 tcctcctctt tccagccttg ttgcaattaa atagtatggc tattttttga gcaaaatgct
     3361 gtccaggatc aattcttcac gccgaattaa caaaatttac atttccttcg cctccgccag
     3421 cgaatgagat tgcgatacaa attttatcca gaactctgta gaatccctgc cctgaccttt
     3481 cctgtaactg aatatttttt aagctatgag caaagtaact ccccagccga aaatcggctt
     3541 tgtttccctt ggctgtccga aaaaccttgt cgattcagag cgtattctca ccgaactccg
     3601 cactgaaggt tatgacgtgg taccgagcta tgacgatgcg gacatggtga tcgtcaacac
     3661 ctgcggcttt attgacagcg cggtacaaga atcactggaa gccattggtg aagcgttgaa
     3721 tgaaaacggc aaggtaattg tgaccggttg tctgggggca aaagaagatc agatccgcga
     3781 agtccacccg aaagtgctgg aaattaccgg gcctcatagc tatgagcagg ttctggagca
     3841 cgttcatcac tacgtgccaa aaccgaaaca caacccattc ctgagcctgg tgccagaaca
     3901 aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa atttcggaag gctgtaatca
     3961 ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac ctggtgagcc gtccgattgg
     4021 cgaagtgtta agtgaagcga aacgtctggt agatgcgggc gttaaagaga ttctggtgat
     4081 ctcgcaggat acttccgcct atggcgttga tgttaaacat cgtactggct tccacaacgg
     4141 cgagccggta aaaaccagca tggtcagcct gtgcgaacag ttatcgaaac tggggatctg
     4201 gacacgtctg cactacgttt acccttatcc gcatgtggac gacgtcatcc cactgatggc
     4261 agaaggcaaa atcctgccgt atctggacat tccgttgcag cacgccagcc cgcgcattct
     4321 caaactgatg aagcgtccgg gttctgtaga tcgccaactg gcgcgcatca aacagtggcg
     4381 cgaaatctgc ccggaactga ccctacgctc aacctttatt gtcggcttcc ctggcgagac
     4441 agaagaagat ttccagatgc tactcgactt cctgaaagaa gcgcgtctgg atcgcgttgg
     4501 ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat gccctgcctg accaggttcc
     4561 ggaagaagtt aaagaagaac gct
//
LOCUS       DAMZET010000254         4579 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.254, whole genome shotgun sequence.
ACCESSION   DAMZET010000254 DAMZET010000000
VERSION     DAMZET010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.254"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1223)
                     /gene="rhsD"
                     /locus_tag="POB34_004528"
     CDS             complement(<1..1223)
                     /gene="rhsD"
                     /locus_tag="POB34_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HDI5921361.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGVFGPGWKAPSDI
                     RLQLRDDGLILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRELTGLADRFGR
                     TLTYRREAAGDLTGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     AFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPAAPLVRYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDAGRVVEQLNPAGLSYRYQYEQDRIT
                     VTDSLNRREVLHTEGG"
     gene            complement(1291..3399)
                     /locus_tag="POB34_004529"
     CDS             complement(1291..3399)
                     /locus_tag="POB34_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDI5921362.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIALTGHPQANLNREWQVVASELHGEQPQAVPGR
                     SGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIIKVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
     gene            complement(4220..4432)
                     /locus_tag="POB34_004530"
     CDS             complement(4220..4432)
                     /locus_tag="POB34_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HDI5921363.1"
                     /translation="MLCFLIYITLPFIQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININTNKNIRQVC"
     gene            complement(4508..>4579)
                     /locus_tag="POB34_004531"
     CDS             complement(4508..>4579)
                     /locus_tag="POB34_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HDI5921364.1"
                     /translation="SAIADAVCQLPKLQEVLKRNEII"
BASE COUNT         1050 a         1302 c         1117 g         1110 t
ORIGIN      
        1 ccgccttctg tatgcagcac ctcacgccgg ttcaggctgt ccgtgacggt gatgcgatcc
       61 tgctcatact ggtagcggta actcaggcct gccgggttaa gttgctccac cacccgcccc
      121 gcatcgtcgt agcggtagcg catctccggc ctgcctgcat aacggtgcgc caccatccgg
      181 cccgggtgct gcgcgtcata cgtgaaagcg cgcacctgcg tattgctgcg gtcatatacc
      241 gccagcagtt caccggcttc cgtatatgtg taccgcacca gtggcgcagc gggcaggctc
      301 tccgggtatg ccgggtcgtg catcagccac accgccgaaa ggcggatacc cctgtcgggg
      361 ccgtattcgg taccgggcag tgtgtcgggg aacgctgagg ctgagagagg gcgggaactg
      421 tcagaagaag atagcgaaga ggtgcgggcc tcttccgcac gctgcgcctg cgtggtcagc
      481 accagacgga actcccgccc ggcaccgtcc gtcacgccgg tgatttcccc ggtcaggtca
      541 ccggcggcct cacgccggta cgtcagcgtc cgcccgaagc ggtccgccag ccccgtcagc
      601 tcccggtacg gcggcagcgg cgctggcagc acgtcctcag cacccggcac ccgctctgac
      661 cagccgagta tccaccacgg cccctgtgcg ctgttggtcg ccaggtaaag atgcgggctt
      721 aaccggatat ccggcggcag cgccccccac agccgcgcca gcgtatggcc gtccggctgt
      781 gctgccttac caccgcgcac cagccacatt gactcactgc ggctgtacac cgcctccccc
      841 ggcagcagcg gctcaaagtg aatgctccgc ccgccgttgt cgttgagtat cagtccgtca
      901 tcacgtagct gtaagcggat atcagaaggc gctttccagc cggggccgaa aacgcccacc
      961 ggtgcaggcg tcttcgtccg gtagctgctg taggtgcggg agagaatgaa cggcagcggg
     1021 ccgggcagcg caaggtccgt ctcgccgggc agcaccttcg ccccaagcag cggatttacc
     1081 gggttgcccg aagtcatccc gcccggacac accgagcacg ccacgccggt gggcgcgcca
     1141 attcttacac ctgccgaacc ctggacaatg ggaccgccat actgagtcat atctccctga
     1201 cgcgccgctg gttttccgct catgattcaa attccttctt ctgtttattt ttaatgctgg
     1261 tttgttactg aattccgaac aggtacggga tcagtactct ctcatatccg gcgtatcctg
     1321 cttttcatca ggggtcttcg gtggggattt cgaggctgca cagttccagt taatcaacag
     1381 tgagtcacca ttaacctgct ccttaccctg caaatgaatt tttgtgccgt tgagaaaaat
     1441 ttgtccgtcc ttcgtcagca ccagccttgc cttaccacag cagagctcca gatgctcacc
     1501 ggcggagtaa atcgcggtct gcccggtatc atcctttttc gttttaccaa cggtgaaggt
     1561 aacgttattt ccgactttga cgtcataacc cagcccgacc ctcaacgatt tatgcaaacc
     1621 gatctgcgag gactgcatca gtgccaccga ggtgttcata atgccaccta ccgtcgtctg
     1681 atacgccacg cccacggtca gcgcacgaac aagtccaatc gtttccacct gaacagaacc
     1741 cactttaatt atctggttac tgcccaccgt ctctatctgg ttaacaccca ccgtctgtat
     1801 ctgattgttg gtaaccgcaa tcatctgatt gttgccaatg gtttcagcat ggttgtttat
     1861 cacatcagtg gtgcggttat tcagcacctc ggtgttcatg ttcttctgcg cgtggatgta
     1921 gacctgttct ttccctgtcg catcgtcaaa cttcagttca ttaaacccgc tgcccttata
     1981 ggttttcgaa cgaatggtca tctgcgtctt cgtccccggc aggctgccgg gtgtgcggtt
     2041 ttcctggtgg taggtacgcc ccataataat cggctggtcc gggtcgccgt tgaggaagtc
     2101 cacaatcacc tcctgaccca ctcgcggtat cgccaggttg ccaaagccgg tgcctgccca
     2161 cgcctgtgcc acacggatcc agcatgaact gtcctggttt gacgggttat agcggtccca
     2221 gttaaatttc acccgcacgc ggccatgttc atcacagaag atttcctcgc ctgccggtcc
     2281 cgtcacgacg gcgctctgcg ggccgtccac cagcggtttc agcaacggct gtggccgcca
     2341 tgtccggtct gccggtatta ccgcaaagtg gttattcagc gtggtacctg aaccactgcg
     2401 tcccggcacc gcctgcggct gctcgccgtg cagttcactt gccaccacct gccattcccg
     2461 gttcaggttc gcctgcggat gccccgtcag cgcaattcgc cgtcccggcc atatctccgg
     2521 agaacggctt gttccgcgcg ccacttctgc gttgtttcgc cagccatcca tctgccagcg
     2581 ggcaaagttc tgcccgtggg cacccttgaa acgtccgggg tagtcataca cttcatactg
     2641 tgtgcgctgg taatcctggt actggccttc ctgatcaaaa cgccctgccc agccgggtcg
     2701 tttaaaggtg tagtccttgg tcaccacgga agaagggcgg atttgtgcgc tgtagcggaa
     2761 ctggctgatg cagagggtgc tcacctcggt acgggtgttc ggattccagg ggatctcaaa
     2821 ggactccggc agataacgca cggtgtcgca cagcaccagg ctctggtcgg tacttttttg
     2881 cgcgtgctcc tcataaaaga agatgccttc ctccgccgcc atccggcaca ggaaatcgta
     2941 atcggtttcg ccgtactgga cacaaaactc acgggaagga tgcggctcgc tgaacagcgg
     3001 actccactcg gtcacaccgt tttccttcag gatcgtagcg aggatgcttt cgatgtcctc
     3061 attctggaag atacggaagt tctggcgcag ccctgtgcgc cacagcggcg ggcacacctt
     3121 catgctgtac agcatctggt ttttgtcgtt ctcccccagt tcaaaccagg tcaccacacc
     3181 tttcacccgg cgctgtacgt catcgccctg ccataccgtc aggtaggcca ttttgtccag
     3241 cacctgcgca aattcaaggg aaagaaactg ctgactgacc agagaaaggt cgagggaaaa
     3301 aagtgaagag agtgactggg tgagatgaaa ggaaaccacc gcaaacgcgt ccggtggcag
     3361 gccgtccact tcaagtgtga aacgtaatcc ggttgacata gcaacctccg tgaaaatgaa
     3421 taataatccg cacatattgc atgtacaaca tgtcgggcgg atgagataag cgagtcgaca
     3481 tctggcgtta ccggggaata tcagaaaaat gtcatggtta tgtgatgatt tagtctggga
     3541 ttttacttca gagaaacggg tgtctgttta gtcaacaaaa taatcacaaa cgcaacggat
     3601 gtgttctcga cgttattcac gacaggctcg tacattacag cgagtaataa aaaacctgat
     3661 atgcaaagtc aaaccaatat tcataatgtc tttttattca caacaataaa taattcaata
     3721 acaatcattg aaatgaatga tgatttgtat aaggaatata cgctaccgcc ccaccctgat
     3781 aacatgatct gcacattgcc atcttaacgt cgttaaacac tggcttaata gtggatattt
     3841 caaattagga atttattaaa ttcacctgat gaatatcata agcaaatagc atagaaaata
     3901 atgacaattc ccccgagggg accatttcca aagcactgca ccaccaccag gtgcccggat
     3961 gatcttgctt cagtggtaag agtacctgat tcattgttcc caaaatgatg ttttccccgg
     4021 ccccgccaat aatgtgcagc tctcacgtga taatctattg attaggttat attaagaatc
     4081 aatatttaaa atacatccat tttcttgaat aaatcaacat gtttatttat caattgctgt
     4141 tttagctctg actcatcaag ctctccatat ttgtcctaca gataattgcg aataaataca
     4201 gagaacgcca tttatccttt tagcatacct gccggatatt tttatttgta ttaatattaa
     4261 ttgcatctcc aactccgagc atatccatca ttaaaaactt tttgagatta tagaatacct
     4321 gtgataacaa gtgaaacatc tgcacaatgt gaatagttag tttcttttca gaggaaatga
     4381 aatagacaag ctgaatgaag gggagagtga tataaatcaa aaaacataac atgaatttaa
     4441 ttaattacgc ccatcattgt ggataaagtt actgaataat aaataacata atcctctggt
     4501 atcccactca aataatttca ttccttttta atacttcttg taatttcggt aactgacata
     4561 cagcatcagc aatcgcact
//
LOCUS       DAMZET010000255         4575 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.255, whole genome shotgun sequence.
ACCESSION   DAMZET010000255 DAMZET010000000
VERSION     DAMZET010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.255"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..82
                     /locus_tag="POB34_004532"
     CDS             <1..82
                     /locus_tag="POB34_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDI5921365.1"
                     /translation="TDDGLPESASRALAKQDLSLLVAKNE"
     gene            complement(133..789)
                     /gene="pphB"
                     /locus_tag="POB34_004533"
     CDS             complement(133..789)
                     /gene="pphB"
                     /locus_tag="POB34_004533"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDI5921366.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFYPETD
                     LLISTGDNIDRGPKSLNVLRLLNQPWFTSVKGNHEAMALDAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK
                     "
     gene            complement(895..3456)
                     /gene="mutS"
                     /locus_tag="POB34_004534"
     CDS             complement(895..3456)
                     /gene="mutS"
                     /locus_tag="POB34_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDI5921367.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            3532..3666
                     /locus_tag="POB34_004535"
     CDS             3532..3666
                     /locus_tag="POB34_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921368.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            3743..4096
                     /locus_tag="POB34_004536"
     CDS             3743..4096
                     /locus_tag="POB34_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HDI5921369.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKTRE"
     gene            complement(4133..>4575)
                     /locus_tag="POB34_004537"
     CDS             complement(4133..>4575)
                     /locus_tag="POB34_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="regulates the expression of formate dehydrogenase H
                     and hydrogenase-3 structural genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HDI5921370.1"
                     /translation="IHLPPLRERPEDIPLLAKAFTFKIARRLGRNIDSIPAETLRTLS
                     NMEWPGNVRELENVIERAVLLTRGNVLQLSLPDIVLPEPETPPAATVVAQEGEDEYQL
                     IVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMKRLGIDKSALI"
BASE COUNT         1061 a         1209 c         1181 g         1124 t
ORIGIN      
        1 cacagacgac ggtttgccgg agagtgccag tcgcgcgctg gcgaagcagg atctctcttt
       61 gctggtagcg aaaaatgaat aatggcctgc aataacattt ggttactcat gcttcacaga
      121 agaagcatga gactacttta ttttataaaa tgacagccgc ccgcttttcg gcgtgccggt
      181 atcaatataa atctggttag cgaacgtctg aatgttatca aacatcatat gtccaaatat
      241 aaaataatca gcgccgttta tttgttgtaa ctcgccatta agcgatttct gcacacgatc
      301 aacaggccag agtaattcgc tctccgctat ttctttacca aagagatatt cactccccgg
      361 ataatctgca tgtgcgatga catattttat gttgtcgtta gtgatttcaa taatatgtgg
      421 aaggtgatgg aatttcagca acagatctat tgcctcttgt tgctctgaat catttaaatc
      481 gaaaaaccag tcaccaccgc tggcaagcca catattgcca tcgccagttt cgaatgcatc
      541 caacgccatc gcttcgtggt tgcctttaac cgacgtaaac cagggttggt ttagcaggcg
      601 cagcacgtta agactcttcg gcccacgatc aatattatcg cctgtggaaa taagtaagtc
      661 ggtttcaggg taaaaagaga gttgatgtaa acgggattgt aataactgat attcaccatg
      721 aatatcacca acgacccata tatggcgata gtgatgggca ttgatttttt gatagcgtgt
      781 agatggcatg gttttaccct gtaaaataag ctttcctatt atacagggta tttttatttg
      841 attcgtcagt tgtcgttaat attcccgata gcaaaagact atcgggaatt gttattacac
      901 cagactcttc aagcgataaa tccactccag cgcctgacgc ggagtcagtg aatccgggtc
      961 gagattttcc agtgcttcga ccgcaggcga agtttcttct ggcactgaca gcaaagacat
     1021 ctgcgtacca tccacttgcg tcgcggcggc gttcggcgaa atgctttcca gctcacgcag
     1081 tttttgccgt gcgcgcttaa tcacctcttt tggcacgccc gccagagctg caaccgccag
     1141 gccgtagctt ttgctcgccg cgccatcctg cacgctatgc ataaaggcaa tggtgtcgcc
     1201 gtgctccagc gcatcgagat gcacgttggc gacgccttcc attttctccg gtaactgggt
     1261 cagctcgaaa tagtgggtag caaataacgt caatgcctta atcttattcg ccagattttc
     1321 cgcgcacgcc cacgccagcg acagaccatc gtaggtggac gttccacgcc cgatctcatc
     1381 cattaacacc agactgtatt cggtggcgtt atgtaaaata ttggcggttt cagtcatctc
     1441 caccataaag gttgagcgcc cggaagccag gtcatctgcc gcgcctacgc gggtaaagat
     1501 gcgatcgata ggtccaatct cgactttttg tgccggtaca tagctgccga tgtaggccat
     1561 cagcgcaatc agtgcggtct ggcgcatata ggtactttta ccgcccatgt tcgggccggt
     1621 aataatcaac atacggcgct gcggcgacag attcagcggg ttagcgataa atggctcatt
     1681 cagtacttgt tcaactaccg gatggcgacc ttcggtaatg cgaatgcccg gtttatcaat
     1741 gaaggtcggg caggtgtagt tcagggtata ggcccgttcc gccaggttaa ccagcacgtc
     1801 gagttccgcc agcgcgctcg cgctctgttg caacgcttcc agatgcggca acagcaggtc
     1861 gaacagctct tcataaagct gtttttccag tgccagtgct ttgccttttg aggtgagaac
     1921 tttatcttcg tactctttta gctctggaat gatgtagcgc tcggcgtttt tcagcgtctg
     1981 gcgacgcatg tagttgatgg gtgccagatg gctttgccca cggctgattt gaatgtagta
     2041 gccgtgcacc gcattaaagc caactttcag cgtgtccagg ccggtacgtt cacgctcgcg
     2101 gacttccaga cgctccagat aatcggtcgc gccgtcagcc agcgcgcgcc actcatccag
     2161 ctcttcgtta tagcccgatg cgataacacc accgtcgcgt accagcaccg gcggtgtgtc
     2221 gatgattgct cgctccagca gatcgcgcag ctcggcaaac tcgcccatct tctcacgtag
     2281 cgcctgtacc ggtgcactat cgacagtttc taactgcgca cgcagctccg gcagttgctg
     2341 gaaagcgtgg cgcatacggg ccagatcgcg tgggcgagca gttcgtaaag ccagacgtgc
     2401 cagaatacgt tccaggtcgc cgacctgacg cagtaccggc tgcaactcgg cggtgaaatc
     2461 ctgcaatgcg ccaatagttt gctggcgctc aagcaacacg cgggtatcgc gcactggcat
     2521 atgcagccag cgtttcagca tacgactacc catcggcgtg acggtgcagt cgagcacaga
     2581 agccagcgta ttttccgcac cgcccgccag gttctgagta atttccaggt tacgacgtgt
     2641 cgcggcatcc ataatgatgc tgtcctgctc acgttccatg gtgatagaac gaatatgcgg
     2701 cagggtcgtg cgttgggtat ctttcgcata ctgcaacaga caaccggcag cacaaagtcc
     2761 gcgcggcgcg ttctcgacgc caaaaccgac cagatcgcgg gtgccaaatt gcagattcaa
     2821 ctgctggcgc gcggtgtcga tttcaaactc ccacagcggg cgacggcgca ggccgcgacg
     2881 gccttcaatc agcgacatct cggcgaaatc ttctgcatac agcagttccg ccggattagt
     2941 gcgttgcagc tctgccgcca tcgtttcgcg gtcggccggt tcgctcaggc gaaaacgccc
     3001 ggagctgata tccagcgtcg cgtagccgaa acctttgctg tcctgccaga tagccgccag
     3061 caggttgtcc tgacgctcct gtaacagggc ttcatcgctg atggtgcctg gcgtaacgat
     3121 acgcacaact ttgcgctcaa ccggaccttt gctggtcgcc ggatcgccaa tttgttcgca
     3181 gatggcaacg gactctccct gattcaccag tttggcgaga tagttttcca ccgcatggta
     3241 gggaatcccc gccatcggga tcggctctcc cgccgaagca ccgcgtttgg tcagtgaaat
     3301 atccagcagt tgcgacgcgc gttttgcgtc gtcataaaac agttcataaa aatcacccat
     3361 ccggtaaaac agcaggatct cgggatgctg ggctttcagc ctgagatact gctgcatcat
     3421 gggcgtatgg gcgtcgaaat tttctattgc actcatgggg ttatgtccgg ttccctgata
     3481 ttaaatgggg tgtgatggtt tttattttcg ttgtaatcac ataaggcgca catgataccg
     3541 gagttaatca tttcagggga agaagcgaac ggcgagttct tgatgctggt tccgggaaag
     3601 atgctgtttt gcagcttttg cgtgatgctt atgaagcaag tacgcaaaat tgcgtcggtt
     3661 gaataaacct cctccttcag agccatagtc ccctttcaaa ttaagttgta atataaatac
     3721 accttaagag tatggaggca gcatgtccgg caagcgtatc tctcgtgaaa aactgacgat
     3781 taaaaaaatg atcgatcttt atcaagcgaa atgcccgcag gcgtcagcgg agccggagca
     3841 ttacgaggcg ttgtttgttt acgcgcaaaa gcggctggat aaatgtgtgt tcggcgaaga
     3901 gaaaccagcc tgtaagcagt gtccggtgca ctgttaccag ccagcaaagc gtgaagagat
     3961 gaaacagatt atgcgttggg ccgggccgcg aatgctgtgg cgtcatccga tcttaaccgt
     4021 gcgtcatctc atcgatgaca agcgtccggt gccagaactg ccggaaaaat atcgtccgaa
     4081 aaaaactcgt gaatagtatg ccggacaggc agatctgtcc ggcaatttgc agttaaatca
     4141 atgccgattt atcaattcca agccgcttca tccgtgacag caaggtcgtg cgtttcagcc
     4201 ccagacgttg cgcagcgcct ttaggcccgg caaccacgcc gttggtttct ttcagcacgc
     4261 gaacaatcaa ctgatattca tcttcgccct cctgggcgac aaccgttgcg gcaggcggcg
     4321 tttcaggttc cggtaaaaca atatctggca atgacagctg cagcacgtta ccgcgtgtta
     4381 gcaataccgc gcgctcaatg acgttttcca gttcgcgtac gttacccggc cactccatgt
     4441 tgctcaaggt gcgcagcgtc tcggcaggaa tgctgtcgat attgcgcccc agacgacggg
     4501 caattttgaa ggtaaaggct ttcgccagca gcggaatatc ttccggacgc tcgcgtagtg
     4561 gcggcaggtg aatcg
//
LOCUS       DAMZET010000256         4550 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.256, whole genome shotgun sequence.
ACCESSION   DAMZET010000256 DAMZET010000000
VERSION     DAMZET010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.256"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..314)
                     /locus_tag="POB34_004538"
     CDS             complement(<1..314)
                     /locus_tag="POB34_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HDI5921371.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLAKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LN"
     gene            652..1854
                     /gene="ackA"
                     /locus_tag="POB34_004539"
     CDS             652..1854
                     /gene="ackA"
                     /locus_tag="POB34_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDI5921372.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            1929..4073
                     /gene="pta"
                     /locus_tag="POB34_004540"
     CDS             1929..4073
                     /gene="pta"
                     /locus_tag="POB34_004540"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDI5921373.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            4263..>4550
                     /locus_tag="POB34_004541"
     CDS             4263..>4550
                     /locus_tag="POB34_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid antiporter"
                     /protein_id="HDI5921374.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFP"
BASE COUNT         1116 a         1227 c         1143 g         1064 t
ORIGIN      
        1 cagttaagga ttgcaggggg taatggcgtt acagaacgct tgcccagcca ccacaatccc
       61 tgcatgggta aacttaaggc gaacagcgca gtggcaactg ccggcccaag ctgaccgccc
      121 agggcaatct gccagcagag agtaaatacg gcgatcggcg gcataaaacg gatcgcataa
      181 cgcgtcatct tgataacgcg attttcgaca aagaccgggg caaggcgttt tgccagtggc
      241 cacgtctttg agtaatgctg tccgcggcga aacaagctaa aaaaattaac agaacgatta
      301 tccggcgttg acatgcttca cctcaacttc acatataaag attcaaaaat ttgtgcaaat
      361 tcacaactca gcgggacaac gttcaaaaca ttttgtcttc catacccact atcaggtatc
      421 ctttagcagc ctgaaggcct aagtagtaca tattcattga gtcgtcaaat tcatatacat
      481 tatgccattg gctgaaaatt acgcaaaatg gcatagactc aagatatttc ttccatcatg
      541 caaaaaaaat ttgcagtgca tgatgttaat cataaatgtc ggtgtcatca tgcgctacgc
      601 tctatggctc actgacgttt ttttagccac gtatcaatta taggtacttc catgtcgagt
      661 aagttagtac tggttctgaa ctgcggtagt tcttcactga aatttgccat catcgatgca
      721 gtaaatggtg aagagtacct ttctggttta gccgaatgtt tccacctgcc cgaagcacgt
      781 atcaaatgga aaatggacgg taataaacag gaagcggctt taggtgcagg cgccgctcac
      841 agcgaagcgc tcaactttat cgttaatact attctggcac aaaaaccaga actgtctgcg
      901 cagctgactg ctatcggtca ccgtatcgta cacggcggcg aaaagtatac cagctccgta
      961 gtgatcgatg agtctgttat tcagggtatc aaagatgcag cttcttttgc accgctgcac
     1021 aacccggctc acctgatcgg tatcgaagaa gctctgaaat ctttcccaca gctgaaagac
     1081 aaaaacgttg ctgtatttga caccgcgttc caccagacta tgccggaaga gtcttacctc
     1141 tacgccctgc cttacaacct gtacaaagag cacggcatcc gtcgttacgg cgcgcacggc
     1201 accagccact tctatgtaac ccaggaagcg gcaaaaatgc tgaacaaacc ggtagaagaa
     1261 ctgaacatca tcacctgcca cctgggcaac ggtggttccg tttctgctat ccgcaacggt
     1321 aaatgcgttg acacctctat gggcctgacc ccgctggaag gtctggtcat gggtacccgt
     1381 tctggtgata tcgatccggc gatcatcttc cacctgcacg acaccctggg catgagcgtt
     1441 gacgcaatca acaaactgct gaccaaagag tctggcctgc tgggtctgac cgaagtgacc
     1501 agcgactgcc gctatgttga agacaactac gcgacgaaag aagacgcgaa gcgcgcaatg
     1561 gacgtttact gccaccgcct ggcgaaatac atcggtgcct acactgcgct gatggatggt
     1621 cgtctggacg ctgttgtatt cactggtggt atcggtgaaa atgccgcgat ggttcgtgaa
     1681 ctgtctctgg gcaaactggg cgtgctgggc tttgaagttg atcatgaacg caacctggct
     1741 gcacgtttcg gcaaatctgg tttcatcaac aaagaaggta cccgtcctgc ggtggttatc
     1801 ccaaccaacg aagaactggt tatcgcgcaa gacgcgagcc gcctgactgc ctgatttcac
     1861 accgccagct cagctggcgg tgctgttttg taacccgcca aatcggcggt aacgaaagag
     1921 gataaaccgt gtcccgtatt attatgctga tccctaccgg aaccagcgtc ggtctgacca
     1981 gcgtcagcct tggcgtgatc cgtgcaatgg aacgcaaagg cgttcgtctg agcgttttca
     2041 aacctatcgc tcagccgcgt accggtggcg atgcgcccga tcagactacg actatcgtgc
     2101 gtgcgaactc ttccaccacg acggccgctg aaccgctgaa aatgagctac gttgaaggtc
     2161 tgctttccag caatcagaaa gatgtgctga tggaagagat cgtcgcaaac taccacgcta
     2221 acaccaaaga cgctgaagtc gttctggttg aaggtctggt cccgacacgt aagcaccagt
     2281 ttgcccagtc tctgaactac gaaatcgcta aaacgctgaa tgcggaaatc gtcttcgtta
     2341 tgtctcaggg cactgacacc ccggaacagc tgaaagagcg tatcgaactg acccgcaaca
     2401 gcttcggcgg tgccaaaaac accaacatca ccggcgttat cgttaacaaa ctgaacgcac
     2461 cggttgatga acagggtcgt actcgcccgg atctgtccga gattttcgac gactcttcca
     2521 aagctaaagt aaacaatgtt gatccggcga agctgcaaga atccagcccg ctgccggttc
     2581 tcggcgctgt gccgtggagc tttgacctga tcgcgactcg tgcgatcgat atggctcgcc
     2641 acctgaatgc gaccatcatc aacgaaggcg acatcaatac tcgccgcgtt aaatccgtca
     2701 ctttctgcgc acgcagcatt ccgcacatgc tggagcactt ccgtgccggt tctctgctgg
     2761 tgacttccgc agaccgtcct gacgtgctgg tggccgcttg cctggcagcc atgaacggcg
     2821 tagaaatcgg tgccctgctg ctgactggcg gctacgaaat ggacgcgcgc atttctaaac
     2881 tgtgcgaacg tgctttcgct accggcctgc cggtatttat ggtgaatacc aacacctggc
     2941 agacctctct gagcctgcag agcttcaacc tggaagttcc ggttgacgat cacgaacgta
     3001 tcgagaaagt tcaggaatac gttgctaact acatcaacgc tgactggatc gaatctctga
     3061 ctgccacttc tgagcgcagc cgtcgtctgt ctccgcctgc gttccgttat cagctgactg
     3121 aacttgcgcg caaagcgggc aaacgtatcg tactgccgga aggtgacgaa ccgcgtaccg
     3181 ttaaagcagc cgctatctgt gctgaacgtg gtatcgcaac ttgcgtactg ctgggtaatc
     3241 cggcagagat caaccgtgtt gcagcgtctc agggtgtaga actgggtgca gggattgaaa
     3301 tcgttgatcc agaagtggtt cgcgaaagct atgttggtcg tctggtcgaa ctgcgtaaga
     3361 acaaaggcat gaccgaaacc gttgcccgcg aacagctgga agacaacgtg gtgctcggta
     3421 cgctgatgct ggaacaggat gaagttgatg gtctggtttc cggtgctgtt cacactaccg
     3481 caaacaccat ccgtccgccg ctgcagctga tcaaaactgc accgggcagc tccctggtat
     3541 cttccgtgtt cttcatgctg ctgccggaac aggtttacgt ttacggtgac tgtgcgatca
     3601 acccggatcc gaccgctgaa cagctggcag aaatcgcgat tcagtccgct gattccgctg
     3661 cggccttcgg tatcgaaccg cgcgttgcta tgctctccta ctccaccggt acttctggtg
     3721 caggtagcga cgtagaaaaa gttcgcgaag caactcgtct ggcgcaggaa aaacgtcctg
     3781 acctgatgat cgacggtccg ctgcagtacg acgctgcggt aatggctgac gttgcgaaat
     3841 ccaaagcgcc gaactctccg gttgcaggtc gcgctaccgt gttcatcttc ccggatctga
     3901 acaccggtaa caccacctac aaagcggtac agcgttctgc cgacctgatc tccatcgggc
     3961 cgatgctgca gggtatgcgc aagccggtta acgacctgtc ccgtggcgca ctggttgacg
     4021 atatcgtcta caccatcgcg ctgactgcga ttcagtctgc acagcagcag taatctcgtc
     4081 atcatccgca gctttgcgct gcggatatct gaaccggaaa taatcactat ttccggtttt
     4141 ttattctctt aatctgcatt aatcctttct gattatcttg cttaactgcg ctgcatcaat
     4201 gaattgcgcc atttcacttt gcatacttac cactttgttt tgtgcaaggg aatatttgcg
     4261 ctatgtccgc aatcactgaa tccaaaccaa caagaagatg ggcaatgccc gatacgttgg
     4321 tgattatctt ttttgttgct attttaacca gccttgccac ctgggtagtt ccggtgggga
     4381 tgtttgacag tcaggaagtg cagtatcagg ttgatggtca aacaaaaaca cgcaaagtcg
     4441 tagatccaca ctcatttcgc attctgacta acgaagcagg cgaacctgag tatcaccgcg
     4501 tacagctgtt cacgacgggc gatgaacgcc cgggcctgat gaacttcccg
//
LOCUS       DAMZET010000257         4425 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.257, whole genome shotgun sequence.
ACCESSION   DAMZET010000257 DAMZET010000000
VERSION     DAMZET010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.257"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1068
                     /locus_tag="POB34_004542"
     CDS             <1..1068
                     /locus_tag="POB34_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HDI5921375.1"
                     /translation="SIEECWFARHLLACFYYNKRSYNKAITLWQRCVEMSPEFADGWR
                     GLAIHAWNKQHDYELAARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENN
                     LEIALKRDDMTAELLNLWHLTGQADKAADIIATRKFHPWEGGEGKVTSQFILNQLLRA
                     WQHLDARQPQQASELLHAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMR
                     CLRLAATGDRTINIHSYYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAK
                     TSIEADFFAVSQPDLLSLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAIN
                     PAWPKAALFTTVMPFIFNYVH"
     gene            1165..2148
                     /locus_tag="POB34_004543"
     CDS             1165..2148
                     /locus_tag="POB34_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDI5921376.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAAEKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVVRNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSHVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVLAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            2171..3682
                     /locus_tag="POB34_004544"
     CDS             2171..3682
                     /locus_tag="POB34_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDI5921377.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            3707..>4425
                     /locus_tag="POB34_004545"
     CDS             3707..>4425
                     /locus_tag="POB34_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5921378.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMIVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGI"
BASE COUNT         1053 a         1033 c         1274 g         1065 t
ORIGIN      
        1 agtattgaag agtgctggtt tgcccgtcat ttactggcct gcttctacta caacaagcgt
       61 agctacaaca aagccattac cttatggcaa cgttgcgtag agatgtcgcc ggagtttgcc
      121 gacggctggc gcgggttagc gatccatgcg tggaataagc aacacgatta tgagctggcc
      181 gcgcgttatc ttgataatgc ttatcagctt gcgccgcagg atgcacgtct gcttttcgaa
      241 cgggatttgc ttgataagtt aagcggagcc acaccggaga aacgactggc gcgtctggaa
      301 aataatctgg aaattgcgct gaaacgcgac gacatgacag cagagttgct caatttgtgg
      361 catctcacgg gtcaggcaga caaagcggcg gacattatcg ccacgcgcaa attccacccg
      421 tgggaaggcg gggaagggaa ggtcaccagt cagtttatcc tcaaccagtt attacgcgcc
      481 tggcagcacc ttgatgccag acagccgcag caggccagcg aactgcttca tgccgcgctg
      541 cattatccgg agaatttaag cgaaggccgt ttaccggggc aaactgataa cgacatctgg
      601 ttctggcagg cgatatgcgc caacgcgcag ggcgatgaaa ctgaagcgat gcgttgttta
      661 cgtctggcgg cgaccggcga tcgcaccatt aacatccaca gttattacaa cgatcagccg
      721 gttgattatc tcttctggca aggaatggcg ctgcgactgc tgggcgaaca acacaccgca
      781 cagcaactgt ttagtgaaat gaaacagtgg gcgcaagaga tggcgaaaac cagtattgag
      841 gcggatttct ttgctgtttc acaacctgat ctgttgtcgc tgtatggcga tttacaacag
      901 cagcataaag aaaaatgcct gatggtggcg atgctggcgt ccgcgggact cggggaggtt
      961 gcgcaatatg agtctgctcg cgctgaattg acggcgatta atccggcctg gccgaaagcg
     1021 gcattattca ccactgtgat gccttttatt tttaactacg ttcactaaac cctattaact
     1081 gtaatatttg agcggcacgc gctgccgctc atcacaaaat aattagaata agccgttgtt
     1141 aataaaaacc ctacaggagg ctctatgcct aaaaaaatga gaacaacccg taatctatta
     1201 ttaatggcta cgctattagg tagcgcttta tttgccaggg ctgcggaaaa agaaatgacc
     1261 attggcgcaa tataccttga tactcaggga tattacgctg gagtgcgtca aggcgttcag
     1321 gatgcggcaa aagattcttc agtacaggta cagttaattg aaactaacgc ccagggtgat
     1381 atttcgaaag aaagtacctt tgttgatacc ctcgtggtgc gtaatgtcga tgccattatt
     1441 ttatcggcag tgtctgaaaa tggcagtagc cgtaccgttc gtcgcgccag tgaagcgggt
     1501 attccggtga tttgctacaa cacctgtatt aatcaaaagg gtgtcgataa atatgtctcg
     1561 gcgtatctgg tcggcgatcc gctggaattt ggtaaaaaac tgggtaatgc tgccgccgat
     1621 tattttattg ccaataaaat tgaccagccg aaaattgccg tcatcaattg cgaagccttt
     1681 gaagtttgtg tgcagcgacg taaaggattt gaagaagtat taaaatccca cgttcccggc
     1741 gcgcaaattg tcgctaatca ggaagggact gttttagata aagcgatttc cgttggtgaa
     1801 aaactaatta tctccacgcc ggatctcaac gccattatgg gtgaatcggg cggagcgaca
     1861 ctcggcgcgg taaaagcggt acgtaatcaa aatcaggccg gaaaaattgc tgttttcggt
     1921 tcggatatga ccactgaaat tgctcaggag ctggaaaaca atcaggtact gaaagcggta
     1981 gtggatattt ccggtaagaa aatgggcaat gctgttctcg cgcaaacatt gaaggttatc
     2041 aataaacaag ccgacggtga aaaagtgatt caggtgccaa tcgatctcta taccaaaacg
     2101 gaagatggta aacagtggct ggcaacgcac gttgatggtc tgccctaatc gctgattcgc
     2161 agaggtccct atgttcacgg caacagaggc agtcccggta gcaaaagtgg tggcaggaaa
     2221 taagcgttac cccggcgtcg ttgcgctgga taacgttaac ttcacgctca ataaaggcga
     2281 agttcgtgcg ctgttaggta aaaacggcgc gggcaaatcg actctcattc gaatgcttac
     2341 cggcagtgaa cgcccggata gcggtgatat ctggattggc gagacgcgac tggaaggtga
     2401 cgaagctacg ttgactcgcc gtgccgctga actgggggtt cgtgcggttt atcaggagtt
     2461 aagtctggtg gaagggctga cggtggcgga aaacctctgc ctcggtcagt ggccccgccg
     2521 caacggcatg attgattacc tgcaaatggc gcaggatgcc caacgttgct tacaggcgct
     2581 gggcgttgac gttagtcctg aacaacttgt ttcaacgcta agcccggcgc aaaagcaact
     2641 ggtggaaatt gcacgggtga tgaagggcga gccgcgcgtg gtcattcttg atgaacctac
     2701 gagttcgctt gccagtgcgg aagttgaact ggtgatcagc gcggtgaaaa agatgtcggc
     2761 actgggcgtg gcggtgattt atgtcagcca ccggatggaa gaaattcgcc gcattgcctc
     2821 ctgtgccacc gttatgcgcg atggtcaggt ggcgggcgat gtgatgctcg aaaacacctc
     2881 cacgcatcat attgtgtcgc taatgctagg gcgcgatcac gttgatattg cgccggtagc
     2941 ccctcaggaa attgtcgatc aggccgtgct ggaagtccgt gcattacgcc ataagcccaa
     3001 gctggaggat atcagcttta cgttacgtcg cggcgaagtg cttggcattg ctggcctgct
     3061 gggggcaggg cgcagtgaat tgctgaaggc gattgttggg ctggaggagt atgaacaggg
     3121 cgaaattgtt atcaacggcg agaaaatcac gcgccccgat tacggcgaca tgctgaaacg
     3181 cggcattggc tatacgccag aaaaccgcaa agaagcgggg atcattccct ggttgggcgt
     3241 tgacgaaaat acagtgctga ccaatcggca aaaaatcagc gccaacggcg tactgcaatg
     3301 gtccaccatc cgccgcctga ccgaagaggt gatgcagcgg atgacggtca aggccgccag
     3361 tagcgaaacg cccatcggca cgctttctgg cggcaatcag caaaaagtgg tgatcggtcg
     3421 ttgggtctat gccgccagcc agattttgtt gctcgacgag ccaacgcgcg gcgtcgatat
     3481 cgaagccaaa cagcagattt accgtattgt ccgtgagctg gctgccgaag gaaaaagcgt
     3541 ggtgtttatc tccagtgaag tggaggagtt gccgctggtg tgcgaccgca tcctgttgtt
     3601 acagcacggt acgttctcgc aggagtttca ctcgccggtc aatgtggatg agctgatgtc
     3661 cgccattctg tctgtgcact gatgaatttt accgaggaac ctgaagatgt ctgcttcgtc
     3721 attaccattg ccgcagggca agagcgtctc gctcaaacaa tttgtcagtc gccatattaa
     3781 tgagatcggt ttgctggtgg tgattgccat tctttatctg gtcttctccc tgaacgcgcc
     3841 tggctttatc tcattgaata accagatgaa cgtgctgcgc gatgccgcca ccattgggat
     3901 tgccgcctgg gcgatgacgc tgattattat ctccggtgag attgatgtca gcgttgggcc
     3961 gatggtggct tttgtctcgg tgtgcctggc atttttgctg caatttgaag ttccgttggc
     4021 gattgcttgt ctgctggtgt tgctgttagg cgcgctgatg ggaacgctcg ccggggtgct
     4081 gcgcggcgtg tttaacgtac caagtttcgt tgccacactg gggctgtgga gcgccctgcg
     4141 cggaatgggg ctgtttatga cgaacgcact gccagtgccg attgacgaaa acgaggtgct
     4201 ggactggctg ggcggacaat ttctcggtgt gccggtatcc gcgctgatca tgattgtgtt
     4261 gtttgcgctg tttgtgttca tcagccgcaa aaccgccttc gggcgctcgg tttttgccgt
     4321 tggcggtaat gctacggcgg cgcagttgtg cggcatcaac gttcgtcggg tgcgcattct
     4381 tatctttacc ctttcgggat tattagcggc ggtgaccggc atttt
//
LOCUS       DAMZET010000258         4384 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.258, whole genome shotgun sequence.
ACCESSION   DAMZET010000258 DAMZET010000000
VERSION     DAMZET010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.258"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1377)
                     /gene="aldA"
                     /locus_tag="POB34_004546"
     CDS             complement(<1..1377)
                     /gene="aldA"
                     /locus_tag="POB34_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDI5921379.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPTNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERIYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGG"
     gene            complement(1574..2374)
                     /locus_tag="POB34_004547"
     CDS             complement(1574..2374)
                     /locus_tag="POB34_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDI5921380.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLKQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(2646..>4384)
                     /gene="hrpA"
                     /locus_tag="POB34_004548"
     CDS             complement(2646..>4384)
                     /gene="hrpA"
                     /locus_tag="POB34_004548"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HDI5921381.1"
                     /translation="TVYGLPIVAARKVNYSQIDPALCRELFIRHALVEGDWQTRHAFF
                     RENLKLRAEVEELEHKSRRRDILVDDETLFEFYDQRISHDVISARHFDSWWKKVSRET
                     PDLLNFEKSMLIKEGAEKISKLDYPNFWHQGNLKLRLSYQFEPGADADGVTVHIPLPL
                     LNQVEESGFEWQIPGLRRELVIALIKSLPKPVRRNFVPAPNYAEAFLGRVKPLELPLL
                     DSLERELRRMTGVTVDREDWHWDQVPDHLKITFRVVDDKNKKLKEGRSLQDLKDALKG
                     KVQETLSAVADDGIEQSGLHIWSFGQLPESYEQKRGNYKVKAWPALVDERDSVAIKLF
                     DNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEKLPNKAKLGLYFNPYGKVLELIDDCIS
                     CGVDQLIDANGGPVWTEEGFAALHEKVRAELNDTVVDIAKQVEQILTAVFNINKRLKG
                     RVDMTMALGLSDIKAQMGGLVYRGFVTGNGFKRLGDTLRYLQAIEKRLEKLAVDPHRD
                     RAQMLKVENVQQAWQQWINKLPPARREDEDVKEIRWMIEELRVSYFAQQLGTPYPISD
                     KRILQAMEQISG"
BASE COUNT         1042 a         1197 c         1022 g         1123 t
ORIGIN      
        1 gccgccaata ccggatttac gccatccggc gtggaagcct tgcatagctt cgaagttttc
       61 acggttgatg taagtttcac caaacttcag ccctttaatg gctttcatcg cgacgttcag
      121 attttgggta tagattgatg aggtcaggcc gtaatcactg tcattagcca ttgagatagc
      181 ctcttccagc gtgtcaaatg cgaccaccgg cagaaccggg ccaaaggttt cctcatgcat
      241 aatcgacatt tcctggcgaa catccagcag caatgtcggc ggataataat atcctttccc
      301 ctctaccgct ttgccaccca acgccactct cgccccttct tctactgcgc gcgccacttt
      361 ttgctcaacc ctttccagcg ccgcggcgtt aatcaacggc cccatcgcaa tgtcgttgcg
      421 ttcagcgggg ttaccaaatt gaaccgcctg catcgcttca cccagccgat tgacgaactg
      481 atcataaatg cctttctgta cataaatacg ttctgcacag ttacacactt gcccactatt
      541 aatgacgcgt gaatcaacga tggctttgac tgccagttca agatcggcat cgtccattac
      601 gatagctggt gctttacccc ccaattccag acacactttg gtgatgtttt tcgccgcagt
      661 cgccatgatc ttctcacctg cagagacgct gcctgtcata ctgaccattg cgacctttgg
      721 gttacccgcc agttcttgcc caacggtttc accacgcccc agtacaaggt taaacacgcc
      781 gcgcggaagg cctatttcat cgacgatttt ggcgaatgca atcgcattgg ttggcgtaaa
      841 ttcactaggt ttaatgacga tggtattacc ggtcaaaaga gcgggagcca ttttgcgggc
      901 aatgaggaag aacgggaagt tccacggcag aatgccggta gtcacaccaa gcgcacgttt
      961 aaacaaaaga atattttctc ctggacgatc gctttgaata atctcgccct cgtaacgccg
     1021 tgcccactcc gccatgtaat cgatatagtc ggcagtaaaa gccacttcga cttcagccag
     1081 ctgctggatc ttgccccctt cttcaacaat cagcgcactg atttcactgg cgcgttcgcg
     1141 gatcccggcg gagattttgc gcaaccaact ggcgcgttca atagcaggca acgcttccca
     1201 ttctggttgt gcacgttctg ctgcatcgat tgccttacgg gcatcctcgg cctgaccatc
     1261 gggtatgcgg gaaatgacag cctctgtagc agggtttacc acatcaatcc atgcgtctcc
     1321 acgccaggta acaaactgtc catcgatata cataggatgt tgaacgggta ctgacatggg
     1381 cgactcctgt gatttatatg tttgttttcg gtgaatgcat tgttaattaa catcaatcat
     1441 cagagagacg gcatcgtgaa aggacaattc tgtgaagggc ttcataaaaa caagtgtaaa
     1501 aggaataaaa ggcagaaata aaagtcagga gtcatgcccg tttgcaaaca agtggttcac
     1561 aaacgggcaa tttttaacgt aacgagcgac tttccatcgc ctcgatgagc accgcatcat
     1621 gtttcagcgt ttgccatgca tggatgactt ctgccggaaa atcaacgtga acgataaaat
     1681 ctcgcccgcg gggaccgtag ccatcgctat catcgcgtaa acgcggcagc tcgccagtga
     1741 gtagtgagag ataacgctca accggccata atccttgtaa ctgattaata aagattacac
     1801 tgtctgcgtt atttcctaac tgaggaacga atccgggata acttaaccag cgtggtgcat
     1861 cgggattgtc cccagtcata cggcggaacg tcgccatcgt gcgccgctgc atggtggggt
     1921 cctgaacaac gatagccgta tgaactcgtt ctacggcctg tttcaatagc gcgatgctaa
     1981 agcgtgcgtt ttcaccgcag tttgttgact ggtcttcaat ccagattttt tcatgcggaa
     2041 tgtgccagaa ctgatgagcg atatccgcca ggatggtcgc ttctgctctg ccagtggtgc
     2101 ggatagtgtt gtagtgcgga tgctgtgcga tggcgctata caaaaaagtt gtcgagtgac
     2161 cgataccacc actaatcagt aaaggaattt gctgatcgcg agcaatctta catgccgcgt
     2221 cgatagtcgg cataaccgca ttgcctgcaa ggatcacgca atcggcctga tacggcacct
     2281 caccggagaa atcatcctgc gccagccact gtccgataac atttatggca tctatagttg
     2341 ccggcgaaag cgtcggaaac ggggtaatgt tcatggttcc tccttatcgt tcccttaaga
     2401 atactgcgtg agataatagt gagaagcgga gcactctgaa aggaggaagt cgtatgtcct
     2461 taaccgccac tccgcaagcc tgacatatct tttaaggaaa tgctgtagat aaacattatt
     2521 catcttgcag cagagaattg tatttttctg aattcctgaa tttttaccct taccgtttta
     2581 gaattaataa aaaacccggc gctaaatgct taccgggttt tttctttatc aggcaaatag
     2641 cagggttaac cgctaatctg ctccatcgcc tgcaaaatac gcttatctga aatcggataa
     2701 ggcgtaccaa gttgttgagc gaagtaacta acgcgcaact cttctatcat ccaacggatc
     2761 tctttcacgt cttcatcctc acgacgtgca ggcggcagtt tgttgatcca ttgctgccac
     2821 gcctgctgga cgttttcgac tttcagcatc tgagcacggt cgcgatgtgg atcaaccgcc
     2881 agtttttcca gccgtttttc aatcgcctgc aaatatcgca gcgtgtcgcc cagccgtttg
     2941 aagccgttac cagtgacaaa tccgcgatat accaacccgc ccatctgcgc tttaatgtca
     3001 gaaagcccca gtgccatggt catatccacc cgccctttca gacgtttgtt gatattgaac
     3061 actgccgtaa gaatttgctc gacctgcttc gcaatatcta ccaccgtgtc gttcagttcg
     3121 gcacgcactt tctcatgcag cgcagcaaag ccttcttccg tccagaccgg gccaccattg
     3181 gcgtcgatca gttgatccac accgcaggag atacagtcgt cgatcagctc cagcactttg
     3241 ccatacgggt taaagtacag tcccagcttg gctttgtttg gtaacttctc atgtaaatac
     3301 ttgatcggcg agggaatatt tagcagcagt agacggcgaa gaccgttcca cattgcctgt
     3361 ttttgctcca gcgggttatc aaacagtttg atcgccacac tgtcacgttc atccaccagc
     3421 gccggccacg ccttcacttt atagttgccg cgcttctgtt cgtagctttc cggcagttgc
     3481 ccaaagctcc agatatgcag cccgctctgc tcgataccgt catccgccac cgcggacagc
     3541 gtttcctgca ctttgccttt cagcgcatct ttcagatcct gtaatgagcg cccttctttt
     3601 agcttcttat ttttgtcgtc caccacgcgg aaggtgattt tcaggtgatc gggcacctga
     3661 tcccagtgcc agtcttcgcg gtcaacggta acgccggtca tccgccgtaa ctcgcgctca
     3721 aggctgtcga gcaacggtaa ctccagcggt ttgacgcggc ctaaaaacgc ttcggcatag
     3781 tttggcgcgg gtacaaaatt acggcgtacc ggtttcggca acgatttaat cagagcaatc
     3841 accagttcgc ggcgcagacc ggggatctgc cattcaaacc cgctttcctc aacctggtta
     3901 agtaacggca gcggaatatg tacggtcaca ccgtcagcat ccgcgccggg ctcaaactga
     3961 tagctcaaac gcagcttgag attgccctga tgccagaagt tcgggtaatc cagcttgctg
     4021 attttttctg cgccctcttt gatcaacatg cttttttcaa agttgagcaa atcaggcgtt
     4081 tcgcggctga cttttttcca ccagctatcg aagtggcgcg cagagatcac atcgtggctg
     4141 atgcgctggt cgtagaactc aaacaacgtt tcgtcatcaa ccagaatatc gcggcgacgt
     4201 gatttgtgtt ccagctcttc cacttcggcc cgcagtttca ggttttcacg gaagaatgcg
     4261 tgacgcgtct gccagtcacc ttccaccagc gcatggcgaa taaagagttc acgacataac
     4321 gccggatcga tctggctgta gttgaccttg cgcgcggcaa caatcggcaa accataaaca
     4381 gtga
//
LOCUS       DAMZET010000259         4383 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.259, whole genome shotgun sequence.
ACCESSION   DAMZET010000259 DAMZET010000000
VERSION     DAMZET010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.259"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..169
                     /locus_tag="POB34_004549"
     CDS             <1..169
                     /locus_tag="POB34_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312433.2"
                     /note="catalyzes the formation of
                     2-dehydro-3-deoxy-D-gluconate-6-phosphate from
                     2-dehydro-3-deoxy-D-gluconate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDI5921382.1"
                     /translation="KVIDTTAAGDSFSAGYLAVRLTGGSAENAAKRGHLTASTVIQYR
                     GAIIPREAMPA"
     gene            complement(265..1761)
                     /locus_tag="POB34_004550"
     CDS             complement(265..1761)
                     /locus_tag="POB34_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDI5921383.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(1982..3268)
                     /gene="dctA"
                     /locus_tag="POB34_004551"
     CDS             complement(1982..3268)
                     /gene="dctA"
                     /locus_tag="POB34_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HDI5921384.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(3451..>4383)
                     /locus_tag="POB34_004552"
     CDS             complement(3451..>4383)
                     /locus_tag="POB34_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDI5921385.1"
                     /translation="RIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGKNQIQ
                     FFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQQPDG
                     SWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQLMHPN
                     MVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDFGMGY
                     AGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVETEAQ
                     RDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
BASE COUNT         1094 a         1192 c         1096 g         1001 t
ORIGIN      
        1 aaaagtgatc gataccaccg cagctggcga ctctttcagt gccggttatc tggcggtacg
       61 tttaacgggc ggcagtgcgg aaaacgcggc gaaacgcggg cacctgaccg caagtaccgt
      121 tattcagtat cgcggcgcga ttatcccgcg tgaggcgatg ccagcgtaaa agcagcatat
      181 gcaaaaggct ggataaagtg ttacccctct atccagctca tccataaata cctgatgatt
      241 gtgctaactc atcaccatca ggcattactg tgcaggtgga atatccgtca cttcaggatg
      301 tacgtcatcc gtggcgacag aggtggtcgc agcggcggta gatggggcca tgatttgatc
      361 ccaggccgcc tgcaacgctt tcatgttaaa ttccggctcg cctttcggct gcagcagtat
      421 taacgccata tcattcgaca actgctgacg cagatcctga tttaacatct ccaccgtcag
      481 gctgttaagg aaatcctgcc gcagtttctg atactgctcc ggcgcgatat cgacgacctg
      541 attttgcaac gaacgcatcc gctgacccat cagaatatcg gtatcagccc gcgcataggc
      601 ggcaaacagt ttctgcagct ccagtttctt ttgcgccact aacgcattga actcttcttc
      661 cggcagacct ttatcgcgaa ccttcgccag ttcacgcgcc accagattaa ggttgctgtt
      721 cagcttgtcg tttggtgatt cgatgttgat ggcacactgc gcacgcagat acagcacacg
      781 gcagtcaaat ccaagaccga tgtctttgct gttactggcg cttaacgctt gctgaacatg
      841 ccagaacagc gcctcgcggg ccaggtccgc acgccagtag cgcagcagtg cggctgattc
      901 acgaatcggc tgccacggcg tatcccacat gatagataac cggtcctgac gcaccgcgtc
      961 agtcataata ctcaccgctt ccgcacgcag cggagaaagc gtcggcaccg gagctggcgt
     1021 ttcacgtttg cctttcagtt cgccaaacgt tttgttgatt tggtcgacaa ccgagcgcgc
     1081 atccacgttt cccaccacca gtagcgtcat tgcatccggg gtgtaccatt tctgatagaa
     1141 atctttaatt ttttccgctt caacgggttg tttcagcgga tcggcaggat cgtgacctaa
     1201 caaggttgat cctttcagac gataacgcca ccagccctct ttagtatcgg caggccaggt
     1261 tgccaccatg tcctgacttt gcagcgcgtg gttgatggtt tctggcgtga tagtcagttt
     1321 gccagtggca tttgccaggt aagagagcgc ctctttcagc aaatcgttac ggttattggg
     1381 caaactcaga ttaaacagcg tagtgtcata cgagacaatt accggcggca tcgggcgttt
     1441 agggtcgatc ccctgctgcc acaatgaacg cgcctgtgct gcgtcaaggc caccgctttg
     1501 cgttagcgca atacgaggga tggcgtgact gtaaccgctc tgttgtgtac tttcggcgag
     1561 cgaaccggta ttaaccagca ggcgaatttc aacacgatcg ctgggacgct ggggggtggt
     1621 cagcacttgc cactgtaaac cgttggaaag cgtcccctgt tgccatgctg gatcaggctg
     1681 gagcgcatct gcctgcacat agccagcagt ggccatcatc agcaaaccgc ccgctaaaag
     1741 tcgaattttt gtgccctgca tgtgaacccc tgatcaacta tcctggtaat aaaaaaactg
     1801 cccgttgtcg gtcagtctaa atgacgttaa aaacacttcg tgttagaccg caagaacatg
     1861 aaaatgtcac ggaagaagtg aaataaacct gaactcaccc aggtggtaag ttcaggcaca
     1921 ggggtcaatt atgcgcaaac acccgcactc ggggaaggga gtgcgggcat aagtgatgag
     1981 attaagagga taattcgtgc gttttgccat ccggcgcacg attattcagc acatcgtcca
     2041 gttttttgtg gtccagttct ttcacccact tagcaacgac aatggtcgct acgccgttac
     2101 cgaccaggtt agtcagcgca cgagcttctg acataaagcg gtcgataccg aggatcagcg
     2161 ccagacccgc taccggcaaa tggcccaccg cagagagcgt cgccgccagc acgataaagc
     2221 cactacccgt tacccctgcc gcccctttag aagaaagcag caacacgatt aacagcgtga
     2281 tttggtggac gatatccatc tgactgttag tggcctgggc gataaacacc gccgccattg
     2341 tcaggtatat cgatgtgcca tcaaggttaa acgagtagcc tgtcgggatg accagcccca
     2401 ccaccgattt acggcagccg agtttctcca tcttgtcgag catacgcggc agcgccgact
     2461 cggaagatga agtccccagt acaatcagca gttcttcacg gatgtagcga ataaatttga
     2521 agatactgaa accagtcgct ttagcgattg aacccaatac cagcaccaca aacaggatac
     2581 aggtaatgta gaaacagata atcagctgcc ccagttgcac cagtgtgccg acgccgtatt
     2641 taccgatggt aaacgccatt gccccgaacg caccaatagg cgccagacgc atgatcatat
     2701 tgatgatgcc gaagatgacc tgcgagaaac tttcgatgac gttaaaaatc agttggcctt
     2761 tgctgcccag acggtggagc gcaaaaccaa acagtacggc aaacagcagc acctgcagaa
     2821 tgttaccgct ggcaaatgcg ccaatgacgc tcgccgggat gacatccata atgaaggcga
     2881 caatgccctg gtctttcgcc tgatcggcgt aaaccgctac cgctttcgca tcaagcgttg
     2941 ccggatcgac gttcattccg gcaccaggct gcacgacgtt aacgatgata agaccaataa
     3001 tcagcgcgat ggtactgaca atttcaaagt aaagcagtgc gactgcgccg gtacgaccga
     3061 ccgccttcat gctttccatg cccgcaatgc ccgttacgac ggtacaaaag atgacaggag
     3121 cgatgatcat cttaatgagc ttaacgaagc cgtcgccaag cggtttcatt tgctcgccta
     3181 tttcaggata gaaatggcca aggagaatac caatggctat cgctgtcagg acctgaaagt
     3241 aaaggctttt aaacagagag gttttcatag ggtgtccttt agtaaaacca caggtcttgt
     3301 aaggttatgg ggtacctgcg gccttaaaat aacacccaga caacatcaca gaaatgtacc
     3361 tggatcataa ttgaaacaaa aaggttaaaa agtttgagct ggctcgcaca aaccagcact
     3421 ttttaaagtt ttgtaatcag tttggggtag ctacttttct tccaggtaac tctcttcgaa
     3481 gatttcaata gggagtgggc gagcaaaaag gaagccctgg gcaataccaa cgcccgcttt
     3541 tgccagccag tcgcgttgtg cttcagtctc cacgccttcg gcaatcattt gtaagttcag
     3601 gctctgcgcc agcatgataa ttgcagcaat catgctgcta tcttccggca agccttcaac
     3661 aaacattttg tcgattttca gtacgtcgat tggcaacgat ttcatatgct gcagctgacg
     3721 cagccctgcg tagcccatgc cgaaatcatc cagcgccacc cgaactccgg cattgcgcag
     3781 cggacggagg attgccaccg cagcatgagg gtcgtcaata cgtcggcttt ctgtcacttc
     3841 cagaatcagt gttcccggct gaatgcgata gcgggttaac agttccagca tatccgccac
     3901 catatttggg tgcatcagtt gcagcgcaga gaggtttacc gacaagggca gcataatgcc
     3961 gcgctcttgc caggctgcaa gcaatcgaca ggactcttcc agcacccagt gaccgacggt
     4021 aaccatcagc ccacagcact caatgcgatc gattaagcca tccggcaggt cccaactgcc
     4081 atccggttgc tggatacgca gtaacacttc cgcactgacc agttttccgc tggtcatctc
     4141 gacctgtggc tgtaaccaaa tagcaaactg atgattttcc agtgcattaa ggatatcgct
     4201 ctcttccgtc aaccgcttct gggcggcttc catctgctgc ggatcaaaga actgaatctg
     4261 attcttgcct ttatggcgag cggtaaatgc cgcagaaata gcgcgactgt aaagctgttc
     4321 ggcggtgaga tcgccgtaga acatcgccac gccaatgcta cagtgcggac ggagttgaat
     4381 acg
//
LOCUS       DAMZET010000260         4375 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.260, whole genome shotgun sequence.
ACCESSION   DAMZET010000260 DAMZET010000000
VERSION     DAMZET010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.260"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..667
                     /gene="cobB"
                     /locus_tag="POB34_004553"
     CDS             <1..667
                     /gene="cobB"
                     /locus_tag="POB34_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDI5921386.1"
                     /translation="AESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARR
                     RQLQQPEIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVR
                     CSQSGQVLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIA
                     IGTSGHVYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLL
                     KGL"
     gene            complement(806..1594)
                     /locus_tag="POB34_004554"
     CDS             complement(806..1594)
                     /locus_tag="POB34_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDI5921387.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(1591..2052)
                     /locus_tag="POB34_004555"
     CDS             complement(1591..2052)
                     /locus_tag="POB34_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HDI5921388.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSRP"
     gene            complement(2110..3156)
                     /gene="potD"
                     /locus_tag="POB34_004556"
     CDS             complement(2110..3156)
                     /gene="potD"
                     /locus_tag="POB34_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HDI5921389.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(3153..3947)
                     /gene="potC"
                     /locus_tag="POB34_004557"
     CDS             complement(3153..3947)
                     /gene="potC"
                     /locus_tag="POB34_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HDI5921390.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLDIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            complement(3944..>4375)
                     /locus_tag="POB34_004558"
     CDS             complement(3944..>4375)
                     /locus_tag="POB34_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDI5921391.1"
                     /translation="PIRIMFTPSAVIIGLVYILLPFMVMPLYSSIEKLDKPLLEAARD
                     LGASKLQTFIRIIIPLTMPGIIAGCLLVMLPAMGLFYVSDLMGGAKNLLIGNVIKVQF
                     LNIRDWPFGAATSITLTIVMGLMLLVYWRASRLLNKKVELE"
BASE COUNT         1147 a         1079 c         1052 g         1097 t
ORIGIN      
        1 tgcggaatca ggtattcgta cctttcgcgc cgcagatggc ctgtgggaag aacatcgggt
       61 tgaagatgtg gcaacgccgg aaggtttcga tcgcgatcct gaactggtgc aagcgtttta
      121 taacgcccgt cgtcgacagc tgcagcagcc agaaattcag cctaacgccg cgcatcttgc
      181 gctggctaaa ctgcaagacg ccctcggcga tcgctttttg ctggtaacgc agaatataga
      241 caacctgcat gaacgcgcag gtaataccaa tgtcattcat atgcatgggg aattgctgaa
      301 agttcgttgt tcgcaaagtg gtcaggttct cgactggacc ggagacgtta cgccagaaga
      361 taaatgccat tgttgccagt tcccggcacc cttgcgcccg cacgtcgttt ggtttggcga
      421 aatgccactc ggcatggatg aaatttatat ggcgttgtcg atggctgata ttttcattgc
      481 cattggtact tccgggcatg tttatccggc ggctgggttt gttcacgaag cgaaactgca
      541 tggcgcgcac accgtggaac tgaatctcga accaagtcag gtcggtaatg aatttgccga
      601 gaaatattac ggcccggcaa gccaggtggt gcctgagttt gttgaaaagt tgctgaaggg
      661 attataaagc aggaagcatt gcctgatgcg ctacgcttat caggcctaca aggggacgca
      721 attaattgaa tttgcgggtt ttgtgggccg gataaggcgt tcgcgcccca tccggcataa
      781 tacacgcgca cttttccggc ctcttttatc gtttactcgt caataacaat gcggcaataa
      841 acgtacagac accaattagc cctgcccaga gtggcgatat tctggtaatc gcaaagctat
      901 tatcgatata caaacataaa gcctgataac ccatattggc aatagtcatc gccactgcaa
      961 attttaacgc ataaataaaa cgagtcgtgt agcaaaagat ccaggaaaat aagataaacg
     1021 tcgcagccca ggcgataaaa cctaatgtgt tggtgctgcc ggggggagca ttttcatgct
     1081 gttgtcctgg atgcggataa tacgtcagtg gcggcgtcgg ttgttttaat atctgtgctt
     1141 gtagaacagc caatgcatta ttgattccct gataccagag atctgcattt aaataagcca
     1201 gcgtgtgatt ttctatcaga aggccgagat gtttatcgtc cagaccactt tcaacctggc
     1261 cgttggtgat cataattaac cgtggttcgc gggtagtaaa tagtaacaga acacttcctt
     1321 ttacattact caatgaagat gacgcatgcg ccagcatcgc tttaccattt tgtctgagac
     1381 tggcttcatt attaatgtcg ggtaaaatca caataaacgg tgcaattccc gtttgttggc
     1441 gaaaaagctc ggctttacgt gcgatctctt cagcatccgg tacagtaccg gtaacatccg
     1501 taaaaagtgt cttcattatt ggaatcaatg gtttactggt tgctaccaga ggcaggagta
     1561 tcagtaaact gagcagtata atgaattttt tcatgggcgg gacttccgtt tcctgcgaga
     1621 ttttttggtg gaggggagcg tgattatcag accgacacta ccgaaaataa tgcctgctat
     1681 gccaacaaag ccccatttaa aaaaattaac ccagggattg tttatttcgg cttgttctgg
     1741 atgccctggc ggataataga cattgactct gtcaccttcg ctatcttcat aaaaatcact
     1801 accgatattc gaattgaaaa ttagcgtgta atctggcgta gggcgaaagg caaccactgg
     1861 ataccaggaa ccattatcat cgacgtcgtt tgagtgattg ttgtaccaaa cggcatcaac
     1921 aatgattcct tctgtatgaa ccgcgctttt ggtaaaagta aaatggtaat aaaagatgat
     1981 tgcagaaata acgagtaata tcagaccgat accgagaaaa gcgcgggata acactttact
     2041 gtcctgactc attagccaca tccttgctaa ataaaaaacg ggcggtaatg ccaccgcccg
     2101 cttgctgaat taacgtcctg ctttcagctt ctgataatac tcttcataaa tgctgctggc
     2161 tgcgccaacg tcattctgcc attcaccatt tttaatggtt tcagcatccg ggtagagcgt
     2221 tttatcgttc gccacttccg ggcttaacag cttacgcgcc gcaaggtttg gcgttggata
     2281 accgatagtt tcggcaacct gttttgccac atccgggcgc agcaggaagt tgatcaattt
     2341 cagcgcgcct tctttgtttt tggcatttgc cgggatcgcc aggctgtcca tccagaaaat
     2401 gccgccttct ttcggccata ccacgtcaat tggcgtaccc gcctggcgtg caacgaaagc
     2461 agaaccgttc cagatcatgc cgaggttaac ttcgccttcc atgtacgggt tagccgggtt
     2521 atcggagtta aaggctgcca cgtttggcat cagttttttc agctcgttat atgcagcttc
     2581 aatctctttc ggatcggtgg tgttaccgga gtagcccagc ttacgcagcg ccatctggaa
     2641 cacttcacgg gcatcgtcgg tcaacagcag gctgcctttg tactcaggct tccacagatc
     2701 ggcccagctg gtgacagatt tcggatccac cgcatcaccg ttaacaccaa tcgccgtcgc
     2761 accccagata tacggaatgg agtaatcgtt attcgggtca aacggcttat tgagcatgtc
     2821 tggatcgaga ttgctgaagt tcgttaactt cgacttgtcg atcttctgga tcatcccttc
     2881 tttacgcatt ttatcgacgt aataggttga aggaaccacc aggtcatagg caccgtcttt
     2941 gtacgttttc agcttggcat acatggtttc gttcgactcg taagtcgaat agataacctt
     3001 aataccggtt tctttggtga actgttcaag cagtcctggc ggcacgtact cggtccagtt
     3061 gtagaaatac agcgtgttgt tgtcatcggc gtgagcggcg ctcatgccca gtgccagagc
     3121 acccgccgcg agcaggtggc gtgaccattt tttcatttaa cgtcccctgt gttacctttc
     3181 gttttatcac gagcaataag ctggctggca ataaccatca ccagcgacag caccagtaat
     3241 atggttgcca gcgcgttaac ttccggcgat acgccgactt tgaccatcga ataaattttt
     3301 aacggcagaa tttcataact tggtccggtg acgaacgaag aaactaccac atcgtccatc
     3361 gacagggtaa agcttaacac ccagcccgcc gccaccgcag gcattgccag tggcagaatg
     3421 atttttcgca gaatggtaaa ttcactggca ccgagatctt tcgccgcttc cagcatccgc
     3481 acgtcaaaac ctttcaggcg cgaatacacc gtcaccacca caaacggcag gcagaaggtg
     3541 atatgcgaga acagcagcga ccagaagcca agctgaatat ccagcagcat aaacagcacc
     3601 agcagagaaa tcgccatcac gatatctggt gacatcatca ccacaaacag cattccgcta
     3661 acgaacggct taccacgaaa gcgataacgg tacagcgcaa ctgccgtcag tgaaccgata
     3721 agcgtagcaa atgtcgccga aaacaccgcc attgttagtg aatgctgcgc tgcctgtaac
     3781 aggctgtcgt tgttcatcag caggctatac catttggtgg taaaaccctg ccagttgatg
     3841 ccaaagcgcg agctgttaaa ggagttcaca atcaaaataa tgattgggat atacaggtac
     3901 gcgtagatag cggtcataaa accgccgcga agcagtcgac cgatcattcg agttccacct
     3961 ttttattcag cagacgagaa gcgcgccagt aaaccagcaa catcaggccc attacgatag
     4021 tcagcgtaat gctggtggct gcaccaaacg gccagtcacg aatattgagg aactggacct
     4081 tgatgacgtt accgatcagc aggtttttcg caccgcccat caggtcggat acatagaaca
     4141 ggcccatcgc tggcagcatc accagcagac atccggcaat aattcccggc atcgtcagtg
     4201 gaataatgat acggataaaa gtctgtaact tgctggcacc gagatcgcgc gccgcctcaa
     4261 gcaacggctt atccagtttt tcgatactgg agtacagcgg catcaccata aacggtagca
     4321 gaatgtaaac cagaccgata atcaccgcac tgggcgtgaa catgatacga attgg
//
LOCUS       DAMZET010000261         4355 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.261, whole genome shotgun sequence.
ACCESSION   DAMZET010000261 DAMZET010000000
VERSION     DAMZET010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.261"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            419..1839
                     /gene="espX1"
                     /locus_tag="POB34_004559"
                     /pseudo
     CDS             419..1839
                     /gene="espX1"
                     /locus_tag="POB34_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
     gene            2092..2868
                     /locus_tag="POB34_004560"
     CDS             2092..2868
                     /locus_tag="POB34_004560"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HDI5921392.1"
                     /translation="MLSDNYNAITLGNGWNTPLGAISFYAARSSSKLNNDTRHEGTSY
                     QVAYNKYLVQTATRFSVAAWRYASQDYRTFSDHLYENDKHYHQSDYDDFYDIGRKNSL
                     SANIMQPLSNNLGNVSLSALWRNYWGRSGNAKDYQFSYSNNWQHISYTFSASQSYDEN
                     NKEEERFNLFISIPFYWGDDIAKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQL
                     NYGIYVNQQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNA"
     gene            3043..3417
                     /locus_tag="POB34_004561"
     CDS             3043..3417
                     /locus_tag="POB34_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921393.1"
                     /translation="MIPHRENHLVLDIANSESETELQGNRQIIAPYRGAVSYVQFTTD
                     QRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGSRLFIRVDEIPTGIKVALNDE
                     QNLFCTITFQHVIDENKTYICQ"
     gene            3430..4355
                     /locus_tag="POB34_004562"
                     /pseudo
     CDS             3430..4355
                     /locus_tag="POB34_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
BASE COUNT         1531 a          795 c          724 g         1305 t
ORIGIN      
        1 tatgatgcgt gagagaacac actaccggtt gccagatagt caaaagatgc tggcttttgc
       61 agacatgccg ttacactttc tgaactcttc gtggcttaag aatcgtagaa ccatcggctg
      121 atacattctg aacaagaata tctactcaat ctccagactc ccctacaaag aatgtggata
      181 ttgtgataca cattgaggta ttaaacttaa gaccactcta tttttgaatt aaattcctat
      241 acactttaac cattcataaa tatctcctta ttagatcttg taaataatta acaacaatca
      301 acacaggtta aatacctatt tatattctac ttcataaaat taatgagcta tttttgatag
      361 tagtcacatt cactcataaa ttaaattaat aaaaattcat caacttcaag gactgataat
      421 gacaaatggt atctcaactt cgccacattg tctttataaa tcaaatatag tagatgatgt
      481 aataataaat aaaacaagac aaaatgagtt agtaaaagta ttttgcgagt ataaaacaga
      541 gttcttaatt ttgtttgatg acttttttag aagccataac ttgcctaaac catctccagt
      601 ccttcatcat ttttttcaat atactcatct gagagatgca tatttttata gatgtaaact
      661 catagaacat acagttcaat tttatttttt taaacacaaa ggtatcacat tacttagatt
      721 ggatgttttt gatgacagaa caagcgaatg tctttctgaa gagattaaga tataccaaga
      781 atgccatgaa aaattcgtta attttttaaa ggcaaacttc aaccaggaaa tatacccaca
      841 gctatatact cctgaaatat tttatgaggc ctgcagaaat ctccagtcat tttatgctca
      901 tcaggagacg tcacaaaacg ctaaatatag tgcaatagac aagaaaaaaa gtcatcttaa
      961 ccaggaaatt aaaaagctaa ttaaaaaaaa tattttccct gagctataca atgagcaatg
     1021 caataaaatc ccctcctcat cgacagatga taaccagcaa ataacatggc agaattttaa
     1081 aactagcaat tcggcatatt caaaaccatg tggaaaatta tctttattta aaagcgtgcc
     1141 cagtcggtta atagaaaaat cagcttactg ctcaactgaa aacatggcca ctaataaatt
     1201 tgatgtcgtc ttttcttact gcggagataa tataaaggaa tttatacttt tattacccta
     1261 taataaatca ctggagatgt atgaacttaa cgatcaaaag atccagtatt tgactgcccc
     1321 taatatcaat atacataaaa tgtcgctatc aaatataaca atagaaaaga gcaacctttc
     1381 atatgggtat tattttggtt gtgtattatc caatatatca tgttttgaaa gtgacttatc
     1441 aaacatcatc ttttcaaatg gagaaataaa caacctcttc attaaaaaag caacatattt
     1501 ggagcatcat tcactaatac cagaataaaa aattttctct gtgaagatat tatgcctggt
     1561 cgatggacca ctcaacttgt caataagcac ttaggttaca gatacacagg agtattcaaa
     1621 accctggcaa gtattgatga caaaccgtcc cgtttcgaaa ttttgatacc attggtacaa
     1681 actttagttc gcgacaatgt aaagctaaat aatgatgtgt ataaagaact taataagttt
     1741 atgcacgatt acgacaaaac atcatcagaa atgagaaaat accttaaaag catcaacgag
     1801 tgtatgttcc ttatgaaaaa catagctcat caaaattaaa aaatctcaaa aaagaagtaa
     1861 tttcagaatg aaattaactg tgatgattat taattcacta cagaggaata aaaaataatc
     1921 tgaagcatac tctgaaacat cctactctct cactctaata atggcacctt gctcttctta
     1981 taaagaaggg aataattaag aatattaaac tttcatcaac ttcacatcaa gaaattaaaa
     2041 attttaaaca ttaatctaaa taataacaac ctgatcacat aatcgggtac gattttatcc
     2101 gataactata atgctataac tctgggaaat ggttggaata caccgctggg tgccatctct
     2161 ttttacgctg cccgatcgag tagtaagtta aataatgata ccagacacga aggtacgagc
     2221 tatcaggtcg cctataataa atatctagta caaacagcca ctcgatttag cgtggccgcc
     2281 tggcgttatg cttcgcagga ttacaggaca ttcagcgacc atctttacga aaatgataaa
     2341 cactatcatc agagtgatta tgatgatttt tatgatattg gcagaaaaaa tagcctttct
     2401 gctaatatta tgcaaccttt atccaataat ctgggaaatg tatcattaag tgcgctttgg
     2461 cggaattact gggggcgaag tggaaatgct aaagattacc aattcagtta ctccaataac
     2521 tggcaacaca ttagttatac tttctctgcc agccaatctt atgatgaaaa taataaagaa
     2581 gaggagcgtt ttaatctgtt tatctccatt cctttctact ggggggatga tattgccaaa
     2641 acacgtcacc aaattaactt atcgaattcg acctcatttt ccaaagatgg ctattcctcc
     2701 aacaatactg gaattactgg catagccggt gaacatgatc agttaaatta tggtatatat
     2761 gttaatcagc aacaacaaaa taatgatacc tcgcttggta cgaatttaag ctggagaact
     2821 cccatcgcca taatagatgg cagctatagt cattctaaaa acgcctagca aagtggtgga
     2881 agtattagta gtggattagt tgtctggccc ggtggtatta atatcactaa ccagttatcc
     2941 gatacatttg caattctgga tgcgcctggg ttagaaggtg cacatattaa tggacaaaaa
     3001 tacaatcgaa caaacagcaa aggccaggtt gtttacgacc tgataatacc tcatcgtgaa
     3061 aaccatctgg tacttgatat agcaaacagt gaaagtgaaa cagaattgca gggcaatcgt
     3121 caaattattg caccttaccg tggagcagtt tcttatgtgc agtttacaac tgaccaacgt
     3181 aagccctggt atatacaggc actgcgtccc gatggttcgc cattaacctt tggctatgac
     3241 gtactggatc tccaggaaaa taatattgga gtcgttggcc agggtagtcg cctttttatt
     3301 cgcgtagatg aaattcccac tggaataaaa gttgctctca atgatgaaca gaacttattc
     3361 tgtactatta cttttcaaca cgttatcgat gaaaacaaaa catatatatg ccagtaaaag
     3421 gagtttacta tgaagtggct tttattaatc accttgagtc tatattcttt tattgtgcaa
     3481 agtgctccct gtgcattgac caatgtcggt gaacaacgtg gaacatatat actcaagcca
     3541 ctgagtatga aaggtaatct aaccgccgaa aaattgaaaa caccggaaat atcattgagt
     3601 gtacagactt gcaagagaca accaacgcca ttaaagttca ttttttaaca cagaacgctt
     3661 taaggtttaa agtcggaagt cgtcattata ttattaattt cccctttgaa tctgacggta
     3721 aaaaaataga gcttccaggg aagtcatctt ataatttaga agaaatgcta tctgcgataa
     3781 atttcaccat tccatattct atcgcatctg tatcagatag ttctaacacc gcagacattt
     3841 ttcctggaac tccttttcct ttgttgttcg aaattgaagt gagttcatgt acagggaata
     3901 atactaattg taatagcgtc cgctttaatt ataatttaaa tactatttta caggtaaatt
     3961 taatgacctg tggttttgaa gatcaatcca tcgacactgg acgatttgta ctgtctcgta
     4021 tcaataatga aaattatcag tttcatcata taagatttga ctgcatccag ggcgagcaag
     4081 gtgaattagt ttttgaaccc agtgatgttg aatattactt tgaaccagtg acgatacagg
     4141 agtcaggaac cagcatatta aaaaatgagt tagaaaacag tgaggacggt gcgggtcaaa
     4201 taggctttca actctccaat gatggtacga atgaaatcca gtatggtaaa agcaattact
     4261 actcttttca gcatccccat gaaggaagta atcagattcc tctatttatt cgcccgcgta
     4321 catatggaaa taatgtctca agcgggcaaa taatg
//
LOCUS       DAMZET010000262         4309 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.262, whole genome shotgun sequence.
ACCESSION   DAMZET010000262 DAMZET010000000
VERSION     DAMZET010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.262"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            18..284
                     /locus_tag="POB34_004563"
     CDS             18..284
                     /locus_tag="POB34_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDI5921394.1"
                     /translation="MLVKRMIDHANAISLEVNISALAIAEAKGKIKNNEVDVVLLGPQ
                     VRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQE"
     gene            399..1721
                     /locus_tag="POB34_004564"
     CDS             399..1721
                     /locus_tag="POB34_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDI5921395.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFFALNYCLTLISPAGLN
                     DLIYTLIQTPLKHMGKNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            1749..2060
                     /locus_tag="POB34_004565"
     CDS             1749..2060
                     /locus_tag="POB34_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDI5921396.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            2116..3789
                     /locus_tag="POB34_004566"
     CDS             2116..3789
                     /locus_tag="POB34_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HDI5921397.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     FTAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            3814..>4309
                     /locus_tag="POB34_004567"
     CDS             3814..>4309
                     /locus_tag="POB34_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDI5921398.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYK"
BASE COUNT         1227 a          842 c          992 g         1248 t
ORIGIN      
        1 cgggcatgtc aaccagtatg ctggttaaac gtatgattga tcatgctaac gctatttcac
       61 ttgaagttaa tatttccgca ttggcgattg cagaagctaa agggaaaatt aaaaataacg
      121 aagttgacgt tgttttactt ggcccgcaag ttcgctttca gaaaccagag attgaagccg
      181 ttgcacaggg gaaaatgcct gtagccgtta tcgagatgaa agactacggc accatgaacg
      241 gacaggcagt tcttgaattt gcgatgaaac tactgcaaga gtaagaatct aatattatta
      301 aactattacc tgaactaact tttcacacat cataaaatgg atatggcata catgctttat
      361 tgggatgtat aaatatccaa aacaacaggt ataaaaaaat gggattaatg gcctcattcg
      421 aacgtggaat ggaacgtttt cttgttccag ttgctatcaa gttaaactca caaaaacatg
      481 ttgcagcggt gagagatgga ttcgttttta cgtttccaat tatcatggca agttcattaa
      541 ttatattaat taactttgcc atattatcgc cagacggttt tattgccgga ttactgcatc
      601 tgaatagcat tttccccaac cttgaaaaag cacaagctat ttttactccg gtaatgaatg
      661 gttctgtaaa tatcatgtca attatgattg ctttcctggt cgccaggaat atggcgatta
      721 gctatgagca agatgatctt ttatgcggat taacggcaat aggagcattt tttattgttt
      781 atacgccata tcagatgata gatgggcaag cattcctgac gaccaaatat ctcggcgcgc
      841 aggggttgtt tgttgctgtt atcgttgcat tgatcaccag tgaaatattt tgtcgcttag
      901 ctcgaaaccc aaaaatcacc ataacgatgc cggcagctgt accgcctgcg gtagcgcgtt
      961 catttaaagt tttattgcca atattttttg tcatggtgtt ctttttcgca cttaattatt
     1021 gcctgacact gatatccccg gcaggactaa acgatcttat ttacacatta atccagacgc
     1081 cgctcaaaca tatgggaaag aatatctttg cggtaattat cctgggggct gtgggtaatt
     1141 tcctgtgggt gctggggatc cacggaccta ataccacatc ggcaattcga gaaaccgttt
     1201 tttctgaggc taatctggag aatctctcct gggccgctca acatggcact acctggggcg
     1261 cgccatatcc gattacctgg acttctatta atgatgcatt cgccaactgc ggcggttcag
     1321 gtatgacgtt ggggttattg ttggctattt ttatcgcttc taagcgtgcg gaataccgtg
     1381 atctggcaaa aatgtcattt atccccggta ttttcaatat caatgaaccg ataatgtttg
     1441 gccttcctat tgtacttaac cccatcatga tggtgccgtt tattatggtt cccattgtta
     1501 actgtgccat tggttacttc tttgtttcga tggaaattat tccaccggtt gcttatgccg
     1561 tgccctggac tacgcccgga cctttaattg ctttcctcgg aaccgggggg aactggctgg
     1621 ctttactggt tggtttttta tgtttaggtg tggcgacaat gatctattta ccttttgtta
     1681 ttgccgccaa caaagtcaat aacatgacaa ctaacggata attccattta cccaggaagg
     1741 agattgtgat gtttgcagat gaagaacttg ttatggagtt actggtcaac gcggggcagg
     1801 cacgttctga cgcgatggaa gccatacgtt gtgccgggca aaaagactgg cagggcgcaa
     1861 ctcagctaat ggccagttct gaatctgcct gtcttcaggc acataagatt cagaccgcat
     1921 taattagtca ggatgaaggc tgtggcaaaa taaaagttaa tttaattctg attcatgcac
     1981 aggatcattt gatgaatgca atattatgcc aggatttagc cagggaaatt atttcattaa
     2041 gaaaagaact ccatgcctga ttttttcctc cagtgcgata gctggaggaa attattatac
     2101 tcttaaggat aagtcatgaa tataaagacg cttaacgtca gccttttgtc tttttctatt
     2161 attacagcat tgtttccatt gaacgcgatg gcaacaaaat taaccataga gcagcgcctt
     2221 gaattgcttg aaaatgaatt gtcgcaaaat aaacaagagc tgaaagcaac acagaatgaa
     2281 ctgggagtat ataaatcccg actttcgacc ttacaaaaaa gcatcacgga aaacaaatat
     2341 aaatcagcct cgcttgctga aatatcagcg acatctcccg tcgctgataa catcaaaaat
     2401 gaaaacggtg aacagaactc gtttaccgca gcgcatacta taaatggatc gcagcaagtt
     2461 gccgttattg aaagtaaagg cgacaaaacc actatcgaaa gcgtgaccct gaaagatatc
     2521 agtaaatata taaaggatga tattgggttc agctatcagg ggtacttccg ctcaggctgg
     2581 ggtactggta atcacggctc accacaaact tatgcagcgg gttctctggg acgttttggt
     2641 aatgagatga gcggttggtt tgacctgacc ttaaatcagc gtgtttataa tcaggacggt
     2701 aaaacggcaa atgcggtcgt tacctatgat ggcaacgtgg gtgagcagta taacgatgcc
     2761 tggtttggtg acagtgccaa tgaaaatatc atgcagttca gcgatattta cctgacaacg
     2821 cgaggttttt tacccttcgc accagaggca gacttctggg tcggcaaaca taaactcccg
     2881 caatatgaga tccagatgct ggactggaaa accttaacca cggatgtcgc cgcgggtgtg
     2941 gggattgaaa actgggcact tggtgtaggg ctgtttgata tgtccttaag ccgtgatgat
     3001 gtcgatgttt actctcgtga tttttcgcgt accagccaga tgaataccaa ttctgtggat
     3061 gttcgttatc gcaatatccc gttatgggat gatgcgacat tatcattaat ggctaaatat
     3121 tccgcaccta ataaaacgga tcaacaacaa gataacgaaa atgacaacag ttattttgaa
     3181 atgaaagata gctggatgct gacttctgtt ttacggcaaa acctgcaacg cgatacgttt
     3241 aatgaattta cgttacaggt tgccaataat tcctatgcca gcagttttgc cagtttctca
     3301 gatgccagta atacgatggc gcatggtcgc tattactatg gtgatcatac caatgggatc
     3361 gcctggcgtt taatctctca gggcgagatg tatcttactg acaatattat tatggctaac
     3421 gcgcttgtct attctcacgg cgaagatgtt tatagttatg aaagtggtgc tcatagtgat
     3481 tttgacagta ttcgcactgt aataagaccg gcctggatct ggaatacatg gaatcagacg
     3541 gggcttgaat taggctggtt taagcaacag aacaaaactc agcagggtgt aacactaaat
     3601 gaatcggctt ataaaacgac actctggcat gcattgaaag tgggtgaaag cattttaggt
     3661 tcacgaccag aaattcgctt ctatggaacg tatatcaata ttctggataa cgaattatcg
     3721 aattttaagt ttaatgagaa cagcaaagac gagtttatgg ccggcatcca ggcggaagtc
     3781 tggtggtaat ctattttagt taaggagttg tgtatgtcag gatttaaaaa aggtttttta
     3841 tggggcggtg cggtagccgc gcatcagttg gaaggtggct ggaatgaagg aggaaaaggc
     3901 atcagcatcg ctgatgtgat gactgctggc gctcacgggg tgccgcgtga agtgacagaa
     3961 ggcgttatcg acgggcttaa ttatcccaat catgaagcaa ttgattttta tcatcgctat
     4021 aaaacagata ttcagttatt tgccgagatg ggattcaaat gctttcgaac ttccattgcc
     4081 tggacacgaa tctttccgca aggtgacgaa caggagccga atgaagaggg tttacaattt
     4141 tatgatgatc tgttcgatga atgccttaag cagggaatgg aacctgtggt gacgctttca
     4201 cattttgaga tgccttatca tctggtgaca aaatatggtg gctggcgaaa ccgtaaactg
     4261 atcgactttt ttatccgctt cgcatcaacg gtcttcacgc gctataaag
//
LOCUS       DAMZET010000263         4301 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.263, whole genome shotgun sequence.
ACCESSION   DAMZET010000263 DAMZET010000000
VERSION     DAMZET010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.263"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(150..2069)
                     /gene="dhaR"
                     /locus_tag="POB34_004568"
     CDS             complement(150..2069)
                     /gene="dhaR"
                     /locus_tag="POB34_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HDI5921399.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPVDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            2297..3367
                     /gene="dhaK"
                     /locus_tag="POB34_004569"
     CDS             2297..3367
                     /gene="dhaK"
                     /locus_tag="POB34_004569"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDI5921400.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            3378..4010
                     /gene="dhaL"
                     /locus_tag="POB34_004570"
     CDS             3378..4010
                     /gene="dhaL"
                     /locus_tag="POB34_004570"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HDI5921401.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGVDGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            4021..>4301
                     /locus_tag="POB34_004571"
     CDS             4021..>4301
                     /locus_tag="POB34_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="phosphotransferaese subunit; phosphorylates
                     dihydroxyacetone along with DhaK/DhaL; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HDI5921402.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVR"
BASE COUNT         1066 a         1109 c         1083 g         1043 t
ORIGIN      
        1 acctctttga tcagaagcaa gtcaacggag gatatcgctt cagcttttaa gtgcgggata
       61 gaccagccct taaacgatgc tgaaaaatgt tagcgtgcgc cggaatgaaa tatagatcac
      121 tctgatgtat cagaaatcgt ctctgtcttt catacccggc gcttaaactg ccctgcatca
      181 atgccatgtt gcttcatttt ccgccacagc gtagtgcggc cgatcccaag taaagccgac
      241 atttcctgaa tgcgaccgcc tgtgacctgg gctgcgttaa taattgcctc tttttcaact
      301 tccgcaaatg acagactggt ggaaaggcgt gtggcgctga catcatctgt tgcctgctcg
      361 gtaaacagat gttccggcaa atcactgacg cgaatgcgcc cattatcact actcagagca
      421 agattctcga tgacgctgta aagttcaaaa tcgttgccag gccatgcaca ggaaaccagg
      481 cgagcgaggg catcgtcatc aattttcagc cgcgtagaga agcgtttttc aagactgcgt
      541 aatttgttat tcaccagcgc cggaatgctg ccacgccgca tacgcagagg cgggatggta
      601 atttcaaatg catgcaacgc gtaatacagc tggcgactaa aacgattttg ttccaccagc
      661 attgcgaggt ccgcggtcgt tgtggcaatc actttgacat caactggtat taaacgccgc
      721 gcatccagtc gcgtgataac accctgcttg ataacctgaa gcaaagcaga ctgtaactcc
      781 accgccagat attcaatctt ttcaagaaac agcgtgccgc cgtgtgccag ttccagccga
      841 ctcagacggc cattttcatt gtccgtgcga tcgccaccaa taaattcttc cgccagcgcc
      901 gcatcaccat ataactcaca attgacggcg atataaggac ctgcagcacg ctcgctttca
      961 ttatgaattg cctgacttag cagtgccttt cccacgccct cttctccaca aagcaggaca
     1021 ggaaagctac tgcgcgccgc ctggcgacca aaatgaatca agcggcgggt ttgcggatcg
     1081 tcctgtggca tatgagcgaa ggtatggctg acttttccta attgactggt catcaactgc
     1141 cgcatctgtt ccacaggatg gagcaacaaa ataaagctgg ttccctgcgt ttctattatc
     1201 ggtttaaggg ttatcaccgc atcaataaac tgatgctgac tttcaaaggt tgcttctacg
     1261 tgtttgagcg gatgtgcctg ttttattgct tgttgcaata cggcgggtaa cgttaagagt
     1321 tcagtgattg cccttccctg acttgccgtc gcgtcaaggc gcaagacccg cgccgcctgg
     1381 gcattaataa attgcaaatt accctgctcg tcccagctaa tcacgccatc atccatactt
     1441 tctaacaggg cattaagttg atttaaatga cggttagttt cagcgagcaa actgtccgtc
     1501 agcagtaaat ttccgacctc gcgggcgatt gccaacgtca acggcaaatc agctgcggta
     1561 gtttgctcaa ccggacacgc cagcgctatt gttcccgtca atcggccctt gctgtcaaac
     1621 aacggcgttg cacaaaaggc ccagttccag agtgcctgtt tgaaatgttg atcggccatc
     1681 gttttcacgg cctgaccaga gatagccgct aacgatagcg cacaagtacc aataattccc
     1741 tcggcgcaat acgtgccgtc attgaatccc agtgcactta gctgctgcaa ggtttgcgga
     1801 tcgccattac ggctgagaat gcaggcggtt tcatcgagga taaacagcgc acactctcgc
     1861 ggtgccatat attcccaggc atcttccagc gcagcctgcc cgagcgtcag catcgctttc
     1921 ttacgccgat aaatagaagc aaatgtcacg ccctgggcct gatgtggtac gttccatgtc
     1981 tcccgtttca tcagcttatt gcatcgctcc caggaggttg caattaaggg agatatgccc
     2041 cgaccatcgt tgttaaaagc gccactcata tcccgcatcc cttatgacaa aaggtgttcc
     2101 ggctcgcaat tttaagtatt aatacacgta aaagtttcaa tatggaacat tcattaaagc
     2161 atatgtttca ctatgaaaca taatttgggc taattttctt ttcccttgcc tgatgcacaa
     2221 tggattcatc ggcagtcagt ggtcgccgtg tcgttgaaca tcatccatgc cctaccgtaa
     2281 ttgctggagc aaaataatga aaaaattgat caatgatgtg caagacgtac tggacgaaca
     2341 actggcagga ctggcgaaag cgcatccatc gctgacactg catcaggatc cggtgtatgt
     2401 cacccgagct gatgcccctg ttgcaggaaa agtcgccctg ctgtcgggtg gcggcagcgg
     2461 acacgagccg atgcactgtg gttatatcgg tcaggggatg ctttcggggg cctgtccggg
     2521 cgaaattttc acctcaccga cgcccgataa aatctttgaa tgctccatgc aaattgatgg
     2581 cggcgaaggt gtactgttga ttatcaaaaa ttacaccggc gatattctta actttgaaac
     2641 agcgaccgag ttactgcacg atagcggcgt aaaagtgacc actgtggtca ttgatgacga
     2701 cgttgcggta aaagacagcc tttataccgc cgggcgacgc ggcgttgcca acaccgtatt
     2761 aattgaaaaa ctcgtaggcg cagcggcgga gcgtggcgac tcactggacg cctgtgcgga
     2821 actggggcgt aagttgaata atcaaggcca ctcaataggt atcgctctcg gtgcctgtac
     2881 cgttcctgcc gcgggcaaac cttcttttac cctggcggat aatgagatgg agtttggcgt
     2941 cggcattcat ggtgagccgg gtattgaccg ccgctccttc tcttcccttg atcaaaccgt
     3001 cgatgaaatg ttcgacaccc tgctggaaaa tggctcatac catcgcactt tgcgtttctg
     3061 ggattatcaa caaggcagtt ggcaggaaga acaacaaacc aaacaaccgc tccagtctgg
     3121 cgatcgggtg attgcgctgg ttaacaatct tggcgcaact ccgctttctg agctgtacgg
     3181 cgtctataac cgcctgacca cacgttgcca gcaagcggga ttgactatcg aacgtaattt
     3241 aattggcgcg tactgcacct cactggatat gaccggtttc tcaatcacct tactgaaagt
     3301 tgatgacgaa acgctggcac tctgggacgc cccggtccac accccggccc ttaactgggg
     3361 taaataagga gaaagcaatg tcactgagca gaactcaaat tgttaactgg ctcactcgtt
     3421 gtggcgatat tttcagcacc gagagcgagt atcttaccgg actggatcgc gaaattggcg
     3481 acgctgacca cgggctaaat atgaaccgag gctttagcaa agtggtggaa aaactccctg
     3541 ctatcgcaga taaagatatc ggtttcattc tcaagaatac cggtatgacg ctgctttcca
     3601 gcgtcggtgg tgccagtggt ccgctgttcg gtaccttctt tatccgcgcc gcacaggcga
     3661 cccaggcacg gcaaagcctg acactggaag agctttatca gatgttccgc gatggcgtgg
     3721 acggcgtaat cagtcgcggg aaagccgaac ctggcgataa aaccatgtgt gatgtgtggg
     3781 tgccggtggt ggaatcgtta cgtcagtcca gcgagcaaaa tctctctgtt ccggtggcgc
     3841 tcgaagctgc cagtagaatc gccgaatccg ctgcacaaag tacgattacg atgcaagccc
     3901 gcaaaggccg cgccagttat ctcggtgaac gcagtattgg tcaccaggat cccggcgcga
     3961 cctcggtgat gtttatgatg caaatgttgg cgttagccgc aaaagagtaa ggaattggtg
     4021 atggtaaacc tggtcatagt ttcacatagc agccgactgg gagaaggtgt cggtgaatta
     4081 gcccgtcaga tgttaatgag tgatagttgt aaaatcgcca ttgccgcggg aattgacgat
     4141 ccacaaaatc ctattggtac cgatgccgtc aaagtgatgg aggccatcga atctgttgct
     4201 gatgccgacc atgtgctggt catgatggat atgggtagcg cattattgag tgctgaaact
     4261 gcgctggaat tgctggctcc caagatcgcc gcaaaagtac g
//
LOCUS       DAMZET010000264         4270 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.264, whole genome shotgun sequence.
ACCESSION   DAMZET010000264 DAMZET010000000
VERSION     DAMZET010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.264"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..269
                     /locus_tag="POB34_004572"
     CDS             <1..269
                     /locus_tag="POB34_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HDI5921403.1"
                     /translation="REIRNRLSSQYNLSNLEPNIQIWNVDLRGDRSLTLRYIPHNRAP
                     LDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMGNL"
     gene            complement(321..1040)
                     /gene="fadR"
                     /locus_tag="POB34_004573"
     CDS             complement(321..1040)
                     /gene="fadR"
                     /locus_tag="POB34_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HDI5921404.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            1261..2802
                     /gene="nhaB"
                     /locus_tag="POB34_004574"
     CDS             1261..2802
                     /gene="nhaB"
                     /locus_tag="POB34_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HDI5921405.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            2948..3478
                     /gene="dsbB"
                     /locus_tag="POB34_004575"
     CDS             2948..3478
                     /gene="dsbB"
                     /locus_tag="POB34_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HDI5921406.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(3524..>4270)
                     /gene="umuC"
                     /locus_tag="POB34_004576"
     CDS             complement(3524..>4270)
                     /gene="umuC"
                     /locus_tag="POB34_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HDI5921407.1"
                     /translation="LMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
BASE COUNT          928 a         1094 c         1090 g         1158 t
ORIGIN      
        1 atcgggagat ccgtaaccgg ttatcgtcgc aatataactt aagtaatctg gagccgaata
       61 ttcagatctg gaacgtggat ttgcgcggcg accgttcgct gacgctgcgt tacattccac
      121 ataatcgcgc accgctggat cgggggcgca aagaagtgct gaagcatgtg catcgcctgt
      181 ggggatttga tgtgatgctc gaacagcaaa acgaagatgg cagcgtcgag ttgctggaac
      241 gttgcccgcc aagaatggga aatctgtaaa aacaacaaaa aacccctcgt ttgaggggtt
      301 tgctctttaa acggaaggga ttatcgcccc tgaatggcta aatcacccgg cagatttttc
      361 tgcatccggt gccaaatctc gccactctca tgcccatagc gacgcactgt ttcgtacacc
      421 tgatcgtgcg cgccttcact gcacaacgcc gacagtttgt ggtagaagcc cagcgccaga
      481 ctgcgcgctt ccggattggc gaaatagtga cgaccaatac gcgtatacag ccctttcatc
      541 ccgttaagaa tcagaccgta aatcgggttg ccggaagcaa acgccaggcc gcggaatatg
      601 ttgtaatcca gctcggcaaa ggcatcggcg tgatcggcca cttcattagc ggtagccagc
      661 acttcctgcg ctttatcggg atgctgacga aacgcggtgc gaataaaaat agtggaaata
      721 ttggtacgca ccgacagcaa attatcaata agctgcggca cactttcgtg atccagtcgc
      781 gccagtgttt caaggatatt taaaccggaa gtttcccaga aattattcac cttcgtcggc
      841 ttgccatgtt gaatggtcaa ccagccatct cgtgccagac gctgtaacac ttcacgtaac
      901 gtagtacgcg ttacgccaat taattctgaa agttcacgtt ctgcgggcaa aatagtcccg
      961 ggagggaagc ggttattcca gatactttca ataatgtact cttccgcgaa acccgccggg
     1021 ctttgcgcct taatgaccat agtgagattt ccataacaca gcaaaacaaa gttggactca
     1081 tcataccaga gggctaacta cgctgatagc agacaaaaag aagaaaaagg gatcaaggct
     1141 tcattttccg catctctttc cccttgacta tcaagacttg cctcactcct gcctgaccgt
     1201 taagctttgc gtttaaacat aaaaacatca ttgattattt gtaaggtaag ggaaaccatc
     1261 atggagatct cctggggccg cgcgctatgg cgcaattttt tgggccagtc ccccgactgg
     1321 tacaaactcg ccctcatcat tttcttaatc gtaaacccgt taattttcct catcagccct
     1381 ttcgtcgcgg gctggttgct ggtagcggaa tttattttca ctctggcgat ggccctgaaa
     1441 tgctacccgc tgctccccgg tggtctgttg gctatcgaag cggtattcat cggcatgacc
     1501 agcgcggaac acgtccgtga agaggtggcg gcaaatcttg aagtcttgct gttactgatg
     1561 tttatggtgg cgggtatcta ttttatgaaa cagctgttgc tgttcatatt tacccgtttg
     1621 ctgttaagca ttcgctccaa aatgctgctg tcgctctctt tttgcgtggc ggctgcgttc
     1681 ctctccgcgt tcctcgatgc cttaaccgtc gtggcggtgg tgatcagcgt tgcagtcggt
     1741 ttttatggta tttatcatcg cgtagcctct tcccgtaccg aagacaccga cctgcaagac
     1801 gatagtcata tcgacaagca ttacaaagtg gttctggaac agttccgtgg ctttctgcgt
     1861 agcctgatga tgcatgccgg tgtcggcact gcattaggcg gcgtgatgac catggtgggc
     1921 gaaccacaga acctgatcat cgctaaagcg gctggctggc attttggcga tttcttcctg
     1981 cgcatgtcgc cggtgaccgt tccggttctg atttgtggcc tgttaacctg cctgctggta
     2041 gagaagctgc gttggtttgg ctacggtgaa acgctgccgg agaaagtccg cgaagtgttg
     2101 caacagtttg acgatcaaag ccgccaccag cgtacccgtc aggataaaat ccgtctgatt
     2161 gtccaggcga ttattggcgt ctggctggtg actgcgctgg cgttgcatct ggcggaagtt
     2221 ggcttgattg gtttgtcagt cattattctg gcgacatcat tgaccggtgt caccgatgaa
     2281 catgctatcg gtaaagcctt caccgaatct ctgccattca ccgcactgtt gacggtgttt
     2341 ttctcggtag tcgcggtgat tatcgaccaa caactgtttt cgccaattat tcagtttgtg
     2401 ttgcaggcat cggaacatgc tcagctgtcg ctgttctata ttttcaacgg tctgctgtca
     2461 tccatttcgg ataacgtctt cgtggggacg atttatatca acgaagcgaa agcggcaatg
     2521 gaaagtggcg ctatcacgtt gaagcaatac gagctgctgg cggtcgccat taataccggt
     2581 accaatctgc cctccgtcgc tacgccgaac ggtcaggctg cgttcctgtt cctgctgacc
     2641 tctgcactcg cgccattgat tcgcctctct tatggccgca tggtgtggat ggccctgcct
     2701 tacaccctcg tactgacact cgtcggcttg ctctgcgtcg agtttacgct tgcccctgta
     2761 accgaatggt ttatgcaaat gggctggata gcaacgcttt gataacaact taccgggcat
     2821 ataaatgccc ggtttgcctt ttcgcccgat aattgtccaa ttgcttccta tttttgctgc
     2881 ctcctggtgg cgcagcgaat gaattggttt aaactgcgca ctctatgcat attgcaggga
     2941 aatgattatg ttgcgatttt tgaaccaatg ttcacaaggc cggggcgcgt ggctgttgat
     3001 ggcgtttact gctctggcac tggaactgac ggcgctgtgg ttccagcatg tgatgttact
     3061 gaaaccttgc gtgctctgta tttatgaacg ttgtgcgtta ttcggcgttc tgggtgctgc
     3121 gctgattggc gcgatcgccc cgaaaactcc gctgcgttat gtagcgatgg ttatctggtt
     3181 gtatagtgcg ttccgcggtg tgcagttaac ttacgagcac accatgcttc agctctatcc
     3241 ttcgccgttt gccacctgtg attttatggt tcgtttcccg gaatggctgc cgctggataa
     3301 atgggtgccg caagtgtttg tcgcctctgg cgattgtgcc gagcgtcagt gggatttttt
     3361 aggtatggaa atgccgcagt ggctgctcgg tatttttatc gcttacctga ttgtcgcggt
     3421 gctggtggtg atttcccagc cgtttaaagc gaaaaaacgt gacctgttcg gtcgctaatc
     3481 ccttcagcgc tcctgcggga gcgctttttt cctgccgcta tatttatttg acccgcagta
     3541 aatcagaact tcgcgttgta taacgtggtg aaagcatggc acgcttcatc tgccattgtt
     3601 gctggatccc ctgcccggca aaatagagtg ttcctctgcc ctctttagca ttcagtgtat
     3661 ccattaccgt catcaattgc tcactcccgg ggcgcggtgc gttgtcatcg aataaattga
     3721 gctgcgcgac tccctgactg aagaaatccc ccagcatcac gcccgctttt tggtagcgat
     3781 ggcccgcttg ccagatggca tccagagatc gcgtagcagc gttaatgata tccctgctgt
     3841 cctgagtggg cgtcagcagt tttaccgacg cgctattgcc gtaataaggt tcattgagcg
     3901 caaatggtga cgtcttaata aatgtggaga taaaccgaca atattgatgc tcgctgcgaa
     3961 gtttttccgc cgcccgggca gcgtaactac aaatggcctg ccgcatcgat ggataatccg
     4021 tgatgcgttc accaaacgag cgggaacaga taatttcctg cttcgtcggt gcaaactctt
     4081 ccagttgcaa acagggttcg ccgcgcagtt cacgcaccgt tctttcgagc acgacattaa
     4141 aatgtttacg gataaaccgg atatctgtat ccgccaaatc gagaacggtt ttgatcccca
     4201 tcgcgtccag ttttttgctg atccgccgtc caatccccca gacgtcatcc acggggagag
     4261 cagacattaa
//
LOCUS       DAMZET010000265         4244 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.265, whole genome shotgun sequence.
ACCESSION   DAMZET010000265 DAMZET010000000
VERSION     DAMZET010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.265"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            1..152
                     /locus_tag="POB34_004577"
                     /pseudo
     CDS             1..152
                     /locus_tag="POB34_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(1102..1899)
                     /locus_tag="POB34_004578"
     CDS             complement(1102..1899)
                     /locus_tag="POB34_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI5921408.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(1909..2460)
                     /gene="ybcL"
                     /locus_tag="POB34_004579"
     CDS             complement(1909..2460)
                     /gene="ybcL"
                     /locus_tag="POB34_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HDI5921409.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(2629..2961)
                     /gene="emrE"
                     /locus_tag="POB34_004580"
                     /pseudo
     CDS             complement(2629..2961)
                     /gene="emrE"
                     /locus_tag="POB34_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
     gene            complement(3295..3609)
                     /gene="fliE"
                     /locus_tag="POB34_004581"
     CDS             complement(3295..3609)
                     /gene="fliE"
                     /locus_tag="POB34_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDI5921410.1"
                     /translation="MSAIQGIEGVISLLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            3824..>4244
                     /gene="fliF"
                     /locus_tag="POB34_004582"
     CDS             3824..>4244
                     /gene="fliF"
                     /locus_tag="POB34_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDI5921411.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRT"
BASE COUNT         1269 a          952 c          843 g         1180 t
ORIGIN      
        1 gtaggtgact gcgacgagat cgaccagata ttgcagtcag atattactac aaaggcgttt
       61 tgtggagtgc tggctatttt gccagtagct gcggaggtgc gccaatatcc gtcatccgcc
      121 aatacattga acaacagcaa acacctggtt aggtggaaaa ccgcgcctta tatccctgcc
      181 ctgaaggacg gggttttacg gcgcaccgga taagcctgga gattatctac accagcatgg
      241 acatcattgt actatgcccc gtacatgccc catcacatcc tcaacatcaa agtattagag
      301 ccttgcaaac cattatgcgt ctcagtttgt ctcatctatc ttacgcaaaa aaaaatagaa
      361 ctaatatcaa gcggcgctat attgcgtatt aaataaaacc acctcaatta cattaataaa
      421 tccaaacaaa catcatgcaa ataatttcca tggttatttc taggctattt aaattaaata
      481 aatcatgagt caatcataaa taacaacctt taatataatt tgactaccaa atttatattc
      541 gttcacaaaa tagtcatgca ttgtgtgcaa tagaaacagt tcagataaag atagggatct
      601 acttcacaat ttaaatgaga aactaaaact tacatcttga aataatctca ttgattggat
      661 gaatatttat agtgcagtga catcattttt aaataatagt tcaaataaaa gggctcacga
      721 tgaaaaaatt aacagtggca atttctgctg tagctgcatc ttaacgtata aaccagattt
      781 aataacaacg aactagattt aataacaacg atatccgtac ctatccggat atctaatgtc
      841 ataagcatcc ctttaattat cccctcaacc tcttgtaact ttcccactct tgaactccac
      901 atagtccaaa cgccggatgc aaaaaaatca cacgtgcatt tctgaaattg tgatggagga
      961 agatagtatc aattatatgt gcttataaac gtccccgatt agcgcagaag tcaccggatt
     1021 gttcaggctc caataggttg agtatgctgt atttattttc caaaataaaa atcgatgctg
     1081 ttgctccttc ataaaatact tttactctct ggacgctttt cttagctctc ttggtgttat
     1141 cttaaaacga ttatggaacc tttcagtaaa acgagaagga cacttgtaac cattttccct
     1201 ggcaatctcg ctgatgggtt ttactgtcgt ttgtatagca gacaacgcat tatttaacct
     1261 cacgtcgtcc aatatacttt gaaaacttac tccctcgctt gccagacggc gatgcaatgt
     1321 ggaaacagaa atgtagagat atctcgctac cttgtttgct gtccattttg ttccaggttc
     1381 ggatagcatt aggttataac aacgacttat taatgattgt ttactatctg ataaaagtaa
     1441 acaattaaca tgatgtgctc ctaatgaaag tagaacgccc attgctaagt gttcctgaat
     1501 ttgcgttgag aaacctcggg aaatagatgt ttttagttgc tcccaacaat atattaactc
     1561 gggattctga ggtaagcaga aacctgtcgt gttacgaatt tgatcggtta tcgcataaag
     1621 attttggaac ttttcaatta gatcaatggg ataataaagc atttctgcaa gataaagacc
     1681 tgcttctggg taattcgcaa tataaaattc atatccacag ggaaataata ttatctgatt
     1741 attatcaaca gttagactat gcgtctccca attgataact ttttttccct gacggatacg
     1801 acataaagct ggcataagag gcttaaccct gtgaatctca tgatgtttat gcatccgtat
     1861 ttcttcaatc tttaggttag tcctatcttt tgccagcata ctcttatcct actttacctc
     1921 ataaacgggt attatctcag cggttgcaat tttattggca ttaagcatat aaccaactaa
     1981 cgctccgctg gaattagaat ccacaggaat cttatcagtt tttagagccc atattttaaa
     2041 ctggtaatga tggggtttat ctcctttggg aggacatgct ccaccaaatc ccgcatagcc
     2101 aaaatcattt cggccttgga cagcaccagt cggaagtttt gtaccatcac gtcttcctgc
     2161 atcagcgggc aaatatgtta ccgttgctgg aatattagca acagtccagt gccaccatcc
     2221 gctgcctgta ggcgcatctg gatcatacac agttacggca aagcttttgg tgccttctgg
     2281 agcaccagac caggttaatg agggcgatgt attacccccc tcacacccaa atccagaaaa
     2341 tacatgagac gttgtaagtt gctctcctgt ttttatttca ttactagtga cctgaaatgc
     2401 tgcggcctgc gcagaaaatg ttatgaatgc caatacactt gaaacgataa gttttttcat
     2461 aaaaacctct ttgttgtgac ctatcgttat tttatttgat gtgcattgat ctcattatgc
     2521 aaaaaggcgc aatgatcgcg caaatgcaat cataattata aagttgcccc gaacattagc
     2581 cataatatgc acaaatcaga ttcctgttat ctcaaggatg attatttttt aattcggtgc
     2641 gcttcgtgac aacaagttaa tgactaacac accggcacaa atcaacatca tgcctatgat
     2701 ggctggcagg tccagtcgtt ggccaaaaaa tccccaggaa agcaaactaa tcagaacgat
     2761 accgactcct gaccagatag cataagcaat ccctgtaggt atataagcca gtgtctgagc
     2821 taataaccaa aatgatgcac aatagcaaat aattgtgcca acagagggct ataaccgtgt
     2881 aaacccctct gaaaacttca ttaaggttgt accaatgacc tctgcaagta ttgcgccacc
     2941 aagataaata taagggttca tagcatattc tttcctgttc atactggaga gaattgtact
     3001 acagtttgaa cacaactcac cgttttcatc ctggtgtatg tgtatccagg gggctctggc
     3061 gtggcaagag ataaaggcag gaacaataat gatcagaagg aaccccaggc taacaaaaga
     3121 gttcactctt cctatgagca aagcgaagac aaaacatatt atcgcgttta cggatcgtgg
     3181 atggcagaaa actttcagga tcaggtatcc attttaaacc agaaattaag tgagtttgcc
     3241 ccatccatgc cccacgcggg cagatgggat gtaattaaca acaggttaca aaacctacac
     3301 ctgcatggac atcacttcct gatacgccgc caccagctta ttacgcacct gaatccccat
     3361 ttgcatagaa actgaggctt tttgcatatc ggtcatcaca tcgtttaacg ccacgccggg
     3421 ttcaccgagg gtgaattttt ctgcctgcgt gcgggcagct gtttgtgtat cgctaatgcg
     3481 atcgagcgcg gcgtgcagct gcccggcaaa actaatggtc ggttgcggca gtgaatcctg
     3541 cgcacgcgca ctcatcgccg tagcctgtaa caggctgata accccttcaa tcccctgtat
     3601 cgctgacatt ttcgtctcct ggataatttc tggtagcaaa gcctaccagt aagtcaataa
     3661 gacaaaggcg ctaaatagca acaaaaaaac gggtttattg gcggatagaa aaaaacgaaa
     3721 gcacaaataa tggcagcgtc aatttttcga gtttgctgac ccgggagtga gtcttgttcc
     3781 actttgccaa taacgccgtc cataatcagc cacgaggtgc gcgatgaatg cgactgcagc
     3841 ccagacaaaa tctcttgagt ggcttaatcg cctgcgtgcg aatccgaaaa ttccattgat
     3901 tgttgccggt tccgcggcag tggcggtcat ggtcgcactg atcctgtggg cgaaagcccc
     3961 cgactaccgc acattattca gcaatctttc cgatcaggat ggtggcgcaa ttgtcagcca
     4021 actgacgcaa atgaatattc cttaccgctt cagcgaagcc agcggcgcta ttgaagttcc
     4081 ggcagataaa gttcacgaac tgcgtctgcg cctggcacaa caaggtttgc ccaaaggcgg
     4141 cgcggtcggt ttcgaactac tcgatcagga aaagtttggt atcagccagt tcagcgaaca
     4201 ggtgaattat cagcgggcgc tggaaggcga gctttctcgt acca
//
LOCUS       DAMZET010000266         4186 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.266, whole genome shotgun sequence.
ACCESSION   DAMZET010000266 DAMZET010000000
VERSION     DAMZET010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.266"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..273
                     /locus_tag="POB34_004583"
     CDS             <1..273
                     /locus_tag="POB34_004583"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="UV-induced DNA repair; converts cyclobutane-type
                     pyrimidine dimers created during exposure to UV ratiation
                     to monomers; light dependent; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HDI5921412.1"
                     /translation="WQWAASTGTDAAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDV
                     PGKAVHEPWKWAQKAGVMLDYPQPIVDHKEARLRTLAAYEEARKGA"
     gene            complement(315..1796)
                     /gene="dtpD"
                     /locus_tag="POB34_004584"
     CDS             complement(315..1796)
                     /gene="dtpD"
                     /locus_tag="POB34_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HDI5921413.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVQMIWLYQALKFRNRALALES"
     gene            2067..2810
                     /gene="ybgI"
                     /locus_tag="POB34_004585"
     CDS             2067..2810
                     /gene="ybgI"
                     /locus_tag="POB34_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HDI5921414.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            2833..3489
                     /gene="pxpB"
                     /locus_tag="POB34_004586"
     CDS             2833..3489
                     /gene="pxpB"
                     /locus_tag="POB34_004586"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDI5921415.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            3483..>4186
                     /gene="pxpC"
                     /locus_tag="POB34_004587"
     CDS             3483..>4186
                     /gene="pxpC"
                     /locus_tag="POB34_004587"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HDI5921416.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVV"
BASE COUNT         1013 a         1033 c         1207 g          933 t
ORIGIN      
        1 tggcagtggg cggcgtctac gggtactgat gcagcgccgt attttcgtat tttcaacccg
       61 acaacccagg gcgagaaatt tgatcgcgaa ggtgagttta tccgccgatg gttaccggag
      121 ctgcgcgatg taccagggaa agcggtgcat gagccgtgga agtgggcgca gaaagcaggt
      181 gtgatgctgg attatccgca accgatagtt gatcacaaag aggcaaggct gcgaacgctg
      241 gcagcgtacg aagaagcgag gaaaggagcc tgataaaacg cagcgagcgt tttatcaggt
      301 atggtgctgg cctattaaga ttccagcgcc agcgcgcggt ttctgaattt cagcgcctga
      361 tacagccaaa tcatctgtac cagtcctaca cacgccagtg cgccccaggt aatttgatca
      421 aatacttcaa tatatgcatt gatggagtag ttgatcgccc cggaagcatc aaacgaagcc
      481 tgcgatgtct gatcggcaat cacgcccgcc agatagttcg cgatcgcgcc agaaagcagc
      541 atgtagatgc cggttaatac gccggtcaca ccggggattt caatgcgcgt aatttgcgac
      601 atggcaaccg ggtcgataaa cagttccgca aagcccatca ccgccaggcc taataccatc
      661 agtggcagag aagagtgacc atacattgcg gaccagcggg cgcttaaggt cagaatgcag
      721 aatccggcgc tcatcaggcc aaggccaaga gcaaatttcc cccagatgcg cacggtacga
      781 ttacccgcga cactctcttt taccacccac gccaggaaca caccgcacag cataactgcg
      841 aaggcattaa tcgactggaa catcgcggtc ggaacggtat aaccaaacat atcgcggtta
      901 acgaagcggt cgatataaag gctaatcgag ctaccgccct gttgtgcgaa ggcccagaac
      961 aacatactga agaaggtgag cgtcacaatc agccccagct ccttccgctg tttctggttt
     1021 tctgctttgc gataaatttt tgccagtaca cccaggccaa taatagtcgc gacaattaag
     1081 gcgtataccg accactcttt ccagaacagt acggtaatca gtgcaggcgt tgccaccagc
     1141 agaaccagca gccatcccca gttcggcagg agaaagtttg tcgcacgcag tacttttttg
     1201 ttaacgccgc gggtatgagt gaaatgacga ttgccacata agaaaatgac cagacccgcg
     1261 atcatgccaa ccgccgccag gccaaagccc atcgcccagc tgtactcttc ctgggcgtaa
     1321 ccacaggcga taggtgcgat aatagacccc acgttacccg ccgcatacat cagcgagaaa
     1381 ccgccatcac gacgcggatc ggttggctca tacagctcgc cgagcagaca gcttacgtta
     1441 gatttaaaca ggccatagcc gcagacgata atcgccaggg acagatagag gaatgacgga
     1501 tggatctcac tggcacccag caccacatga ccaatcgcca tcaacaacgc ccccagcatc
     1561 accgccatgc gattgccgag aactttatcc gccaaaaagc caccgaggat tggcgtgaca
     1621 tacaccagcg aacagtaggc gctaaataac tcgtaggcgt gagtatcgtt gtattttagt
     1681 tgattggtga gatagagaat cagcagggca cgcatgccgt aaaagctaaa atattcccag
     1741 atttgcagcg caacgacata gtaaatagcg cgcggttgtg atgcgtgttt attcataata
     1801 attccacaat tgacggcaac gtcacgtaat agttgcgcgg cgtgcttgtg tttccttaaa
     1861 gttaaaactt tgatacagat ctgattattt atgcaatatg ctgtctgatt gcataaatat
     1921 acattagctg aagcgtgatg atataaacaa tttgcgtctt ttggcgtcgg gtttgtttct
     1981 taatatgtca aaagattggc tatcgacgaa caggcgaggt aacgctatgt taacggtgta
     2041 gctatcagaa tacggaaggc gcgaagatga aaaacaccga actggaacaa ctgattaacg
     2101 aaaaactgaa cagcgcggcg attagtgatt acgcgccgaa tggtttgcag gtggaaggca
     2161 aagagacggt gcaaaaaatt gttaccggtg tcaccgccag ccaggctttg ctcgatgagg
     2221 cagtgcgtct gggcgcggat gcggtcattg tgcatcatgg ctacttctgg aaaggagagt
     2281 ctccggtcat tcgcggaatg aagcgtaacc gtttaaaaac gttgctggcg aatgatatca
     2341 acctgtatgg ctggcatttg ccgcttgacg cacatcctga gctgggcaat aacgcacagc
     2401 tggcggcgtt actggggatc acggtcatgg gcgaaattga gccgttggtg ccgtggggag
     2461 agctgaccat gcctgtgccg ggactggaac tagcctcctg gattgaagcg cgtctgggac
     2521 gtaagccgtt atggtgtggc gataccggac ctgaggtggt ccagcgcgtc gcctggtgca
     2581 cgggcggcgg gcaaagtttt atcgatagcg ccgcgcgttt tggcgtggat gcttttatta
     2641 ctggcgaagt ttctgaacag accattcatt cagcccgcga gcagggattg catttttatg
     2701 ctgcaggtca ccatgccact gaacgtggtg gtattcgcgc attgagcgag tggctgaatg
     2761 aaaataccga tcttgatgtg acctttattg atattcctaa tcctgcataa cgaataatca
     2821 gagggatcga aagtgcaacg agcgcgttgt tatctgatag gtgaaacggc ggtagtgctg
     2881 gaactggaac cgccggtgac gctggctagc cagaaacgga tctggcgact ggcgcagcgt
     2941 ctggtggata tgccgaatgt ggttgaagcc attcccggca tgaacaatat tacggtgatt
     3001 ttgcgtaatc ctgagtcgct ggcgctggat gccatagagc gtttgcaacg ctggtgggag
     3061 gagagcgagg ccctggaacc tgagtctcgc tttattgaaa ttccggtggt ttacggtggt
     3121 gcaggcggac cggatttggc ggtggtcgcg gcgcattgcg ggttgagcga aaaacaggtt
     3181 gttgaattgc actcctccgt ggaatacgtg gtctggtttt taggttttca accgggcttc
     3241 ccgtatctcg ggagtttgcc ggaacaacta cacacgccac ggcgcgctga accgcgctta
     3301 ctcgttccgg caggttctgt cgggatcggc gggccgcaga ctggtgttta tccgctggca
     3361 acgccgggtg gctggcagtt gattggtcat acctcactca gcctgtttga tccggcgcgt
     3421 gacgaaccca tcttattacg tccgggagac agcgtgcgct ttgtaccgca gaaggaggga
     3481 gtatgctgaa gattattcgt gcgggcatgt ataccactgt gcaggatggc ggtcgtcacg
     3541 gttttcgcca gtcgggtatc agccactgcg gcgcactgga tatgcccgcg ttacgcattg
     3601 ctaacctact ggtgggtaat gacgccaatg cccccgcgct ggagatcacg ctcggtcagt
     3661 taactgttga gttcgaaact gatgggtggt ttgctctgac gggtgccggt tgcgaagcgc
     3721 ggctggatga taatgccgtc tggaccggct ggcgattgcc gatgaaagca ggccagcgtt
     3781 taacgcttaa acgcccgcag cacgggatgc gcagttatct ggcggtcgcg ggtggtattg
     3841 atgttccgcc ggtaatgggg tcatgcagca ccgatctcaa cgtggggatt ggcgggctgg
     3901 aaggccgttt actgaaggat ggtgaccgac tcccgattgg caaatcgaag cgtgatttta
     3961 tggaagcgca gggcgttaaa cagctgctgt ggggcaaccg cattcgcgcc ttgccggggc
     4021 cggaatatca tgagttcgat cgcgcctcgc aggatgcatt ctggcgttcg ccctggcagc
     4081 ttagcccgca aagtaaccgc atgggctatc gcttacaggg gcaaatttta aaacgcacca
     4141 ccgatcgcga actgttatct cacggtttgt taccgggcgt ggtgca
//
LOCUS       DAMZET010000267         4141 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.267, whole genome shotgun sequence.
ACCESSION   DAMZET010000267 DAMZET010000000
VERSION     DAMZET010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.267"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..435
                     /locus_tag="POB34_004588"
     CDS             <1..435
                     /locus_tag="POB34_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HDI5921417.1"
                     /translation="RLLAANTPVAATVRGNPVYALQHVGPALFSVKHEAADSVHDPSR
                     MEPKERKDWYQSENERIKLEKEQRKLIPVDEVVIVYSSMRKAVVQVLETIPDVLERDC
                     ALTPQAVGVVQQAIDDLRYTLQEKSYEACAAELIPDEEGESL"
     gene            444..2543
                     /locus_tag="POB34_004589"
     CDS             444..2543
                     /locus_tag="POB34_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015987600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDI5921418.1"
                     /translation="MGFSSARNLGRDISAGFSPPRRMPISEAVKKFMRVPKGAGNSVP
                     WDPELTPYIIEPMNCLASREYDAVIFIGPARTGKTIGLIDGWIVYTIVCDPSDMLVVQ
                     MTEDKAREHSKKRLDRTFRSSAAVKKRMSPRRNDNNVHDKTFRDGSFLKIGWPSVNIM
                     SSSDYRFVALTDYDRFPENIDSEGDGFSLASKRTTTFMSAGMTLVESSPGRDICDSKW
                     RRKSPHEAPPTTGILSLYNRGDRRRWYWSCPHCGEYFQPAMDAMTGYRNEPDPFKASE
                     AAYLLCPHCSGIITAEKKRELNSAGVWLREGQVIDRNGNVSGEPRRSRIASFWMEGPA
                     AAYQTWAQLVYKLLTAEQEYEATGSEETLRAVINTDWGLPYLPRASMEQRKSELLEQR
                     AEPVPSRSVPDGINFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRSD
                     CDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDNA
                     YKFWRRCRRDGLGKRIYLFKGDSIRRAKLISRTFPDNTGRTGRRAQAAGDVPLWLLQT
                     DALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSRDGKWSKPGRGANEAFD
                     LMVYAEALVILHGYEKIRWPDAPEWASRETWLECVQDSTEPSSSPEPVSTPVKKQKRK
                     KTVTDDVNPWLTSGGWL"
     gene            2540..2752
                     /locus_tag="POB34_004590"
     CDS             2540..2752
                     /locus_tag="POB34_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921419.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            2680..>4141
                     /locus_tag="POB34_004591"
     CDS             2680..>4141
                     /locus_tag="POB34_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011703754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDI5921420.1"
                     /translation="MGAPPCGSDYTTTGASRVPAGEVLMAILDDVIGVFSPGWKAARL
                     RSRAVIQAYEAVKTTRTHKARRENRTADQLSQYGAVSLREQARYLDNNHDLVIGVFDK
                     LEERVVGKNGIIVEPHPVLRNGAIARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLM
                     LRTWLRDGEVFAQMVSGRINSLTPSAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDD
                     WGRPEKYLVYKSRPVSGRQMETKEVDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSAL
                     KEYEDSELTAARIAAALGMYIRKGDGQSYESDGNGSKENERELTIQPGIIYDDLKPGE
                     EIGMVKSDRPNPNLETFRNGQLRAVAAGSRLSFSSTARNYNGTYSAQRQELVESTDGY
                     LILQDWFIGAVTRPMYRAWLKQAVASGVIRLPRGLDRSSLYTAVYSGPVMPWIDPVKE
                     AEAWKIQIRGGAATESDWVRAGGRNPDDVKRRRKAEI"
BASE COUNT          963 a         1008 c         1238 g          932 t
ORIGIN      
        1 cggcttctcg cggccaacac tcctgtggct gcgactgtca gggggaaccc cgtttacgcc
       61 ctgcagcatg tcgggccagc cctgtttagt gtgaagcatg aggcagcaga ctctgttcat
      121 gatccatccc gtatggagcc gaaagagaga aaggactggt accagtctga aaatgaaagg
      181 atcaagctgg aaaaggagca gcgaaaactc atccccgttg atgaagtagt catcgtctat
      241 tcgtccatga gaaaggctgt cgtccaggtt ctggagacaa ttccggatgt tcttgaacgc
      301 gattgcgccc tgactcctca ggccgtcggc gttgtacagc aggcaattga tgacctgcga
      361 tacactcttc aggaaaaatc ctacgaggct tgtgctgctg aattaattcc tgatgaggaa
      421 ggagagagtc tctaggagga ataatgggtt tttcatcagc ccgaaatttg ggaagggaca
      481 tatcggcagg attttcccca ccacgtcgca tgccgatttc ggaggctgtt aaaaaattca
      541 tgcgtgttcc caagggggct ggtaactcgg tgccatggga tcctgaactg acaccctaca
      601 tcattgagcc catgaactgc ctggcatcgc gtgaatacga tgcggtgatt tttattggtc
      661 ctgcgcgaac agggaagacc attggtctga tcgatggatg gattgtctat accatcgttt
      721 gcgatccttc ggacatgctc gttgtgcaga tgaccgaaga taaggcccgc gagcattcta
      781 aaaagcgcct cgacagaacg ttcagaagca gtgcggcggt aaagaaaaga atgagtccac
      841 gtcgtaacga caataatgtc catgataaga cgttcaggga tggctcgttc cttaaaattg
      901 gttggccctc ggtcaacatt atgtcgtcgt cggattaccg gtttgtcgcc ttaaccgatt
      961 acgaccgttt tccggagaat atcgacagcg agggtgatgg tttctccctg gcctcaaaac
     1021 gtaccaccac atttatgtcc gccgggatga ctctggtgga gagctcgccg ggacgtgaca
     1081 tctgcgacag caaatggcga cgtaagtcgc ctcatgaagc gccaccgacg actggtattc
     1141 tttcccttta caatcgtggt gaccgccgcc gctggtactg gtcatgtccg cactgcggtg
     1201 aatattttca gccagccatg gatgccatga ccggctaccg taatgaaccg gatcccttta
     1261 aagccagtga ggcggcgtat ctactttgcc cgcactgcag cggcattatc actgcggaga
     1321 aaaagcgtga gctcaatagt gctggagtct ggttgcgtga aggtcaggtc attgatcgta
     1381 acggcaacgt ttccggtgaa ccgcgccgct cccgtatcgc cagtttctgg atggaagggc
     1441 cagctgctgc gtatcagacc tgggcgcaac tggtttacaa attactgact gcagaacagg
     1501 agtatgaagc gacaggaagc gaagaaacac tcagggcggt tatcaacacc gactggggat
     1561 tgccttatct tcctcgtgcc agcatggagc aacgaaaaag tgaactgctt gagcagcggg
     1621 cagagccagt tccttcccgc agtgtgccgg atggcattaa tttccttgtg gcgacagtgg
     1681 atgtgcaggc gggacgtcat cgccgttttg tggttcaggt aacgggctat ggcagtcgtg
     1741 gcgaacgctg gattattgat cgttacaaca tcacgcagtc attgcgcagt gactgcgacg
     1801 gggagagcca gcgaattgat ccggccagct atccggaaga ctgggatgtc ctgctgacgg
     1861 atgtttttca taaaagctgg ccgctggcct ccgatccttc tcaacaaatg cgactgatgg
     1921 caatggcggt ggactccggc ggtgaagacg gggtcactga taatgcctat aaattctggc
     1981 gtcgttgccg tcgtgatggc cttggtaaac gtatttacct gtttaagggt gacagcatcc
     2041 ggcgcgcaaa actgatcagc cgtacattcc ctgataacac cggacgaacg ggccgccggg
     2101 cgcaggccgc aggtgatgtg ccgctctggc ttcttcagac ggatgccctg aaagaccggg
     2161 tgaataacgc gttatggcgt gactcgccag gtccaggcta tgtgcatttc cctgactggc
     2221 tggggagctg gttttacgac gaactgacgt atgaagagcg gagccgtgac gggaaatgga
     2281 gtaagccggg tcgcggtgcc aacgaagctt ttgacctgat ggtgtatgcc gaggctctgg
     2341 tcattctgca tggatacgaa aagatccgct ggccggatgc accggagtgg gcgagccggg
     2401 aaacctggct ggagtgtgtc caggacagta ccgaaccgtc atcctcaccg gaaccggtat
     2461 ccacgcctgt taaaaaacaa aaacggaaga aaacagtaac tgacgatgtt aacccctggc
     2521 tgacttccgg aggatggtta tgaaccagaa tgatatcgaa gccatgattc agcgttatac
     2581 ggaagctgaa atggcggtgc tggacggaaa atccgtcact tttaatggtc agcagatgac
     2641 catggaaaac ttatctgaga tccggcaggg gcggcaggag tgggagcgcc gccttgcggc
     2701 tctgattaca cgacgacggg ggcatcccgg gtaccggctg gcgaggttct gatggcaatt
     2761 cttgatgatg tgattggcgt tttttcacca ggatggaaag cggcaaggct gcgttcccgt
     2821 gcggtgatcc aggcttatga ggccgtaaaa acgacgcgga cacacaaagc ccgacgggag
     2881 aaccgaactg ccgaccagtt aagccagtac ggggccgtgt cgttacgtga gcaggcccgt
     2941 taccttgata acaaccacga tctggtcatt ggtgtatttg acaagctgga agaacgggtg
     3001 gtggggaaaa acgggattat tgtcgagcca catccggtat tacgcaatgg ggccattgcc
     3061 cgtgatctgg cagcggagat acgcacccga tggagtgaat ggtctgtcag tccggaagtc
     3121 accgggcagt ttacccgtcc gatgctggaa cgtctgatgc tgcgtacctg gctgcgcgat
     3181 ggtgaggtgt ttgcccagat ggtttccggg cgcataaaca gcctgacgcc ttctgccggt
     3241 gttcatttct ggctggaggc gctcgagcca gactttattc ccatgaccag tgatgagagc
     3301 aacaggctga atcagggcgt gtttgttgat gactgggggc gtcccgaaaa atatctggtg
     3361 tataaaagtc gtcctgtatc cggacggcag atggaaacca aagaagtgga tgcagagcga
     3421 atgctgcatc ttaaatttgt tcgccgtctg caccagatgc gcgggacgtc tttgttgtcc
     3481 ggtgtgctga tccgcctcag tgccctgaaa gagtatgaag attctgagct gactgcagca
     3541 aggatcgccg ctgctctggg gatgtacatc cggaaaggcg acgggcaaag ctatgaatcg
     3601 gatggtaatg gcagcaagga gaatgaacgc gagcttacca ttcagccagg cattatttac
     3661 gacgatctga aacccggcga agaaatcgga atggtgaagt cggatcgtcc caatcctaac
     3721 cttgaaactt ttcgtaatgg tcagttgcgt gccgtggcgg cgggcagtcg tctgagtttt
     3781 tccagtacgg cacgcaacta taacggcact tacagcgccc agcgtcagga actggttgag
     3841 tctactgatg gctacctgat cctgcaggac tggtttattg gtgccgtcac ccgcccgatg
     3901 taccgtgcct ggctgaaaca ggctgtggca tccggtgtta tcaggctacc ccgcggtctg
     3961 gaccgatctt cactgtatac cgcggtgtat tccggaccgg tgatgccgtg gattgaccct
     4021 gttaaggagg ctgaggcctg gaaaattcag attcgtggtg gagcggcgac agaatcagac
     4081 tgggtacgtg caggtggtcg taatccggat gatgtcaaac gtcggcgcaa ggccgaaatt
     4141 g
//
LOCUS       DAMZET010000268         4140 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.268, whole genome shotgun sequence.
ACCESSION   DAMZET010000268 DAMZET010000000
VERSION     DAMZET010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.268"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..134)
                     /gene="tuf"
                     /locus_tag="POB34_004592"
     CDS             complement(<1..134)
                     /gene="tuf"
                     /locus_tag="POB34_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDI5921421.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     R"
     gene            complement(249..324)
                     /locus_tag="POB34_004593"
     tRNA            complement(249..324)
                     /locus_tag="POB34_004593"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(289..291),aa:Thr,seq:ggt)
     gene            complement(331..405)
                     /locus_tag="POB34_004594"
     tRNA            complement(331..405)
                     /locus_tag="POB34_004594"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Gly,seq:tcc)
     gene            complement(522..606)
                     /locus_tag="POB34_004595"
     tRNA            complement(522..606)
                     /locus_tag="POB34_004595"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(570..572),aa:Tyr,seq:gta)
     gene            complement(615..690)
                     /locus_tag="POB34_004596"
     tRNA            complement(615..690)
                     /locus_tag="POB34_004596"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(655..657),aa:Thr,seq:tgt)
     gene            1052..2002
                     /gene="coaA"
                     /locus_tag="POB34_004597"
                     /gene_synonym="panK"
     CDS             1052..2002
                     /gene="coaA"
                     /locus_tag="POB34_004597"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDI5921422.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2031..2996)
                     /gene="birA"
                     /locus_tag="POB34_004598"
     CDS             complement(2031..2996)
                     /gene="birA"
                     /locus_tag="POB34_004598"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HDI5921423.1"
                     /translation="MKDYTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(2993..4021)
                     /gene="murB"
                     /locus_tag="POB34_004599"
     CDS             complement(2993..4021)
                     /gene="murB"
                     /locus_tag="POB34_004599"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDI5921424.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAEALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1073 a         1031 c          921 g         1115 t
ORIGIN      
        1 cgagcagcac cgccgtaggt tttagccagt acggtagtga ttgcagcggt cagcgttgtt
       61 ttaccatggt caacgtggcc gatagtaccg acgttaacgt gcggttttgt acgttcaaac
      121 ttttctttag acatcgattg tccctctaag acacggataa atcggtgata tcaccacatc
      181 aaccaggcaa catgcccgac ttgttgaatg caataaacag aaggaaaaaa cagggaggag
      241 aaaagaagtg gtgctgatag gcagattcga actgccgacc tcacccttac caagggtgcg
      301 ctctaccaac tgagctatat cagcacatct tggagcgggc agcgggaatc gaacccgcat
      361 catcagcttg gaaggctgag gtaatagcca ttatacgatg cccgcatcct ggaactcggc
      421 tacctgattt tcattctgca ctgaatatcg agagaagctc tctttattcg agccggtaag
      481 cgaacttatc gtctcgggct acgccatcgc gtggccgaaa ttggtggtgg gggaaggatt
      541 cgaaccttcg aagtctgtga cggcagattt acagtctgct ccctttggcc gctcgggaac
      601 cccaccggac ttgatggtgc cgactaccgg aatcgaactg gtgacctact gattacaagt
      661 cagttgctct acctactgag ctaagtcggc atcaagtagc gcgcattcta tggagacatg
      721 cgagttcatg caactaaaaa attgcataat ttgttttatt ggtcacattt tatgcgacac
      781 gatgaagaaa cagccgaacg gagggcagat tagcacactt tttcaacatc attgtgctca
      841 acaatgcgct cctgctaaac cataattctt tttatcagat ggaatatctg tcacattgct
      901 tttcaacgat agcttcctgg cagagatttt ttcttattat tcctccccat ctggtgttac
      961 cctcctgccc attaacccat tcaacagaac tgtgacgcgc catggcaaat atcgctttgc
     1021 cgatagagct atgaccgcca gaaacatgct tatgagtata aaagagcaaa cgttaatgac
     1081 gccttaccta cagtttgacc gcaaccagtg ggcagctctg cgtgattccg tacctatgac
     1141 gttatcggaa gatgagatcg cccgtctcaa aggtattaat gaagatctct cgttagaaga
     1201 agttgccgag atctatttac ctttgtcacg tttgctgaac ttctatataa gctcgaatct
     1261 gcgccgtcag gcagttctgg aacagtttct tggtactaac gggcaacgca ttccttacat
     1321 tatcagtatt gctggcagtg tcgcggtggg gaaaagtaca accgcccgtg tattgcaggc
     1381 gctattaagc cgttggccgg aacatcgtcg tgttgaactg atcactacag atggcttcct
     1441 tcaccctaat caggttctga aagaacgtgg tctgatgaag aagaaaggct tcccggaatc
     1501 gtatgatatg catcgcctgg tgaagtttgt ttccgatctc aaatccggcg tgccaaacgt
     1561 tacagcccct gtttactcgc atcttattta tgatgtgatc ccggatggag ataaaacggt
     1621 tgttcagcct gatattttaa ttcttgaagg gttaaatgtc ttacagagcg ggatggatta
     1681 tccacacgat ccacatcatg tatttgtttc tgattttgtc gatttttcga tatatgttga
     1741 tgcaccggaa gacttacttc agacgtggta tatcaaccgt tttctgaaat tccgcgaagg
     1801 ggcttttacc gacccggatt cctattttca taactacgcg aaattaacta aagaagaagc
     1861 gattaagact gccatgacat tgtggaaaga gatcaactgg ctgaacttaa agcaaaatat
     1921 tctacctact cgtgagcgcg ccagtttaat cctgacgaaa agtgctaatc atgcggtaga
     1981 agaggtcaga ctacgcaaat aattttcagg ggagctaatg ctcccctttc ttatttttct
     2041 gcactacgca gggatatttc accgcccatc cagggtttta ttattccatc ctgctcaagt
     2101 aataaagccc cctgtttgtc tattccgcgt gaaatgccaa atatttcttt atcaccaatg
     2161 ataagtttca ctgggcgatt aataaaatta tccagctttt cccagcgcga cagataaggt
     2221 gccaatcctt cttgttcgaa gagttccaac gcagcacgta attcacgtat tagcatggcc
     2281 gccaacgtat tacgatcgag attgatcccc gcttcctgca gcgtgatcca cccctgatta
     2341 acgacactct cttcaacacg gcgcattgcc atgttgatcc cggctccaat gactatttgc
     2401 gccgcatcgc cagttttgcc agtcagctcc acaagaatgc ctgccagctt gcgatcctgc
     2461 agatagaggt cattaggcca tttgacacga actttatctg ctcccagctt gcgtaatact
     2521 tccgccatca cgataccgat aaccagactt aaaccaatcg ccgccgccgg gccttgttcc
     2581 agacgccaga acatcgacaa atataagttt gcgccaaaag gcgaaaacca tttccgcccc
     2641 cggcgaccac ggccagcctg ctggtattct gcaacacagg catcgcccga tttaagctct
     2701 ccgatacgat caagaaggta ctgattcgtg gagtcaataa ctggtagcac ggctacacta
     2761 ccgccatcca gctgacccaa tatctgttca gcattaagta actggatggg ctcaggcagg
     2821 ctgtatcctt tacccggaac ggtaaagaca tcaacgcccc agtcacgcag tgtctgaatg
     2881 tgtttattaa tagccgcccg gctcattccc agcgtttcac ccaactgctc gccagagtga
     2941 aattcaccgt tcgctaacag ggcaatcaat ttcagtggca cggtgtaatc cttcatgaaa
     3001 ttgtctccac tgcgctcact tcacctgatg caccaataaa gcggacttca ggctcaagcc
     3061 agacattaaa tttttcaccc actttttgtc ttacatggtg tgccagttgc accacatctt
     3121 cgctttttgc attgtcttca ttaatgagaa ctaacgcctg ttgacggtgc accgcagccc
     3181 cacccatttg catccctttt agctggcact gatcaataag ccaacctgct gccagtttta
     3241 ctgaaccatc cgcctggggg taatttggtg ctgttggaaa ttgtgacagt aatgcttcag
     3301 ccgtttcggc agatacaaca gggtttttga agaaactacc ggcattgcca ttcacttttg
     3361 gatcagggag tttggtggtg cgcatatgac acaccgcatt aaatacttgc tgtggcgtta
     3421 ctgttgtagg atccagacga gttaagtcac catacgttag tacaggttgc cactcttttg
     3481 gcagacgcag acctacggct acaatggcga agcgatcctg gtattcatgt ttaaaaatac
     3541 tgtcgcgata gccaaaacgg cactctttgg cagttaagcg cacttgcttg cctgtcgcca
     3601 gttcaacaca atcaacataa gcgcaaactc gctgtaattc tacgccataa gcaccaatat
     3661 tctggatagg tgatgagccg acacaaccag gaattaatgc cagattttcc agaccaggca
     3721 taccttcctg caaagtgtat tttaccaggc gatgccagtt ttctccggct cctacatgta
     3781 aataccacgc atcaggttca tcatgaattt cgataccttt gatccggttg atgatcaccg
     3841 tgccgcgata gtcttccaga aaaagtacat tacttccttc acccagaata agaacgggtt
     3901 gtccttctgc ggttgcatgc tgccaggcat tgagtaattg ttgttcgtct tcggcacata
     3961 caatgtgctg agcattatga tcaatgccaa atgtgttcca gggttttaag gagtggttca
     4021 tagctgcttt cctgatgcaa aaacgaggct agtttaccgt atctgtgggg ggatggcttg
     4081 tagatatgac gacaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct
//
LOCUS       DAMZET010000269         4099 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.269, whole genome shotgun sequence.
ACCESSION   DAMZET010000269 DAMZET010000000
VERSION     DAMZET010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.269"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..695)
                     /locus_tag="POB34_004600"
     CDS             complement(<1..695)
                     /locus_tag="POB34_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane domain protein"
                     /protein_id="HDI5921425.1"
                     /translation="MSYFYRSKNISGVSINEDSLISLSTAIVERCVKMNEQAQKESRE
                     VCAQCVFFILFDGKGYKVDSIGELVKYFRQATRIDQINFTIETYQSRQSNRMNGSWME
                     LRIDERNSNSSTMIVASEDNEWVDSLWITIQDLLNKCKNKFRFFRTAWTMLSIQISGV
                     TVGFLLSLWTASKLAPKLSIDGAFALSFICIFILFSNLWSFAIPLIIKLIDFLFPSVK
                     FVINGKNYFHWSV"
     gene            complement(905..1174)
                     /locus_tag="POB34_004601"
     CDS             complement(905..1174)
                     /locus_tag="POB34_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921426.1"
                     /translation="MKLTSIHLEILRRVNELQPKDSRIQAASLPHLSAKEVNFAISDL
                     ADIGFIDVIRSKSAEGGLIANEITSKGFAFLSEIGAEKNSSMSTH"
     gene            complement(1389..1730)
                     /locus_tag="POB34_004602"
     CDS             complement(1389..1730)
                     /locus_tag="POB34_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001614563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDI5921427.1"
                     /translation="MPCVIRVKELCQHCHGKGEVSTACRGCKGKGIVLDEKRTRLHGT
                     PVYKICGRCSGNRFSRLPTTLARLHVQKLVPDLTDYQWYKGYADVIDKLVTKCWQEEA
                     YAEAQLRRVTR"
     gene            complement(1681..2061)
                     /locus_tag="POB34_004603"
     CDS             complement(1681..2061)
                     /locus_tag="POB34_004603"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HDI5921428.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELEGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGEGTMSALPW"
     gene            complement(2061..2144)
                     /locus_tag="POB34_004604"
                     /pseudo
     CDS             complement(2061..2144)
                     /locus_tag="POB34_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            2240..2550
                     /locus_tag="POB34_004605"
                     /pseudo
     CDS             2240..2550
                     /locus_tag="POB34_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2565..2927
                     /locus_tag="POB34_004606"
     CDS             2565..2927
                     /locus_tag="POB34_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921429.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVDRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGR"
     gene            2993..3817
                     /locus_tag="POB34_004607"
     CDS             2993..3817
                     /locus_tag="POB34_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HDI5921430.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKVLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            3945..>4099
                     /gene="yfdR"
                     /locus_tag="POB34_004608"
     CDS             3945..>4099
                     /gene="yfdR"
                     /locus_tag="POB34_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HDI5921431.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAV"
BASE COUNT         1156 a          969 c          843 g         1131 t
ORIGIN      
        1 tggacactcc agtgaaagta atttttacca ttaataacaa acttaacact aggaaacaaa
       61 aagtcgataa gcttaattat taatggaata gcaaagctcc ataagttcga aaacaaaata
      121 aagatacaaa taaatgataa agcaaatgca ccatcgattg atagttttgg cgccagtttt
      181 gaggctgtcc ataagctaag caaaaagcca actgtcacac ctgaaatctg gatacttaac
      241 atagtccaag ctgttctaaa aaatctaaat ttatttttgc acttgttaag taaatcctga
      301 atcgtaatcc ataacgagtc aacccactca ttatcttccg atgctacaat cattgtgctg
      361 ctatttgaat tcctctcatc aattcgtagc tccatccaag atccattcat cctgttagac
      421 tgtcgacttt gataagtttc aatagtaaaa tttatttgat caatcctagt ggcctgtcta
      481 aagtatttca caagctcacc gatagaatca actttatacc ccttaccatc gaacaaaata
      541 aaaaaaacac attgagcaca aacttcccta ctttcctttt gagcttgctc attcatttta
      601 acgcagcgtt cgacaattgc cgtgcttaag ctaatcaagc tatcttcatt tatagaaaca
      661 cctgaaatat tcttgcttct gtaaaaatat gacactttcg attccactcc gcaccagtct
      721 cgatttagcg caggttaaca taaccacttc attggtagaa gaaaagaact cttcttgtca
      781 gaaagctact gtgatatccc taaacctgca gatgatacag aacaaaattt aagcatcaat
      841 tgtaggcgat tttaaattca ctttacagtt taggctgcca attggcagcc gattgaagca
      901 gaaatcaatg agtgctcatc gatgaatttt tttcagcgcc aatctcactc aaaaaagcaa
      961 aaccttttga cgttatctca ttagcaatca atccaccttc ggcagatttg cttcgtataa
     1021 catcgataaa acctatatca gcaagatctg aaatagcaaa gttaacctct tttgctgaga
     1081 gatgaggtaa tgatgcagcc tgaattctag aatcctttgg ctgcaattca ttcactcgac
     1141 gtagtatttc aagatggata gaggtcagtt tcataatgtt ctcctgtttt taatgttgtt
     1201 ttacagaaaa cattataaca cattgatttt gatgtagatt gaatagaaaa aaaccataaa
     1261 aaaacctcgc aatagcgagg tcgtaaaaat tgttcaacga tagatacaca agccccatcg
     1321 ttgagaaaat cttatccata ttttttgaga aatacaagta ttatgtcgtt atcttcggcg
     1381 aaaatcgctt atctcgtcac ccttctcaat tgtgcttcag cgtaagcttc ctcctgccag
     1441 cactttgtaa ccagtttatc aatgacatct gcatatcctt tgtaccactg ataatccgtc
     1501 aggtctggta ccagcttctg gacatgaagc cgcgccagtg tggttggtaa acggctaaac
     1561 cggtttccac tgcaacgccc acaaatctta taaacaggcg tgccatgaag ccgggttctt
     1621 ttttcatcca ggacaatacc tttaccctta caccctctgc acgcggtgct gacttctccc
     1681 ttaccatggc aatgctgaca tagttccttc acccttatca cgcatggcac tggcatgttt
     1741 catgatgtaa cgcatgccgg tgatgtattg cccctcatgc tgactggcgc ggctgtatgg
     1801 gggattacca aaggcagcac cttcaagctc cgcaagacgt tctgaccagt catgcgccag
     1861 cgcgttgtct tccgccgtgt aatacgcagc acatttggcg ttatcaccgt cagtgaacag
     1921 atccagaaca aacgggccaa acagggtgtt aattccccag aaaatgttat ccggcgtgcg
     1981 ccactgatcg cccacttcct tcagttcatg ggctggtttg ttccgcagtt ccaccagcgc
     2041 ctggcaatat ttattactca ttaagccccc acgtaattcc ctgacagata ccactcttca
     2101 cccgatgcag cgcgcttgct gcttttccgt aagcaccgct ccttgttgtt ggggaataac
     2161 caggtccacc tcgcctgatg tggctaaaag caggcacata acagctaagt attttcaacc
     2221 agagagaatc cttagcgttg tggtgaatgc ggctcagcgc acgcgggtta aggttgaggc
     2281 tgacagtcga ccttctgtgg atacccaccc gtctggtgtg caaccttcgc caggcaccgg
     2341 gaggcacccg gcaccacaac tttatgctgt gtgtagtcct ggcggtacca gtttgtaccc
     2401 ttgcttccgg ctggtaccgc tctttttaca aaacagagaa gagcatcacc ggacgacggg
     2461 ctcataaccc aatccatccg ggcggctgcc accgcaggtg ttcttctctg ttttgtggag
     2521 aaactaatcg gccttgcagg gtcgatatga tgaggagcag caaaatggct agcgaacgca
     2581 gtactgatgt gcaggcattt atcggggagc tggacggcgg cgtatttgaa accaaaatcg
     2641 gcgcagttct cagtgaagtc gcttccggtg tgatgaacac gaaaaccaaa gggaaggtct
     2701 cactcaatct ggaaatcgaa ccgtttgatg agaaccgtgt gaaaatcaaa cacaaactct
     2761 catatgttcg cccgactaac cgcgggaaaa tttccgaaga agacaccacc gaaacgccaa
     2821 tgtatgtcga tcgcggtggt cgcctgacta ttctgcagga agaccaggga caattactga
     2881 ctcttgccgg tgaacctgac ggaaaactcc gcgcagcagg tcgttaatat cgttcgtaat
     2941 aaactgatta tttatctcat cactgaatat ctttatatag tgaggactta ttatgtctca
     3001 gaacttagac gcaaccgcaa ttaatcaaat ccatgccctt atttctgctc agggtgttaa
     3061 tgaaattatc agtaagattg gtgccgatgc tgtggcattg cctgagaatt tccgcattca
     3121 tgatctggaa aaatttaatt taaatcgctt ccgtttccgt ggtgcgcttt ccactgccag
     3181 catcgatgac tttacccgtt attctaaagt tcttgcagat gaaggcaccc gctgctttat
     3241 cgatgccgat aatatgcgtg ccgtcagtgt gcttaacctg ggtactattg atgaaccagg
     3301 tcacgcagat aacaccgcca ctctcaaact gaaaaagaca gcaccgttct ctgctctgtt
     3361 gtctgttaac ggcgagcgta actcccagaa gtcactggca gaatggattg aagactgggc
     3421 cgactacctt gtgggctttg atgctaatgg tgacgccatt caggcaacaa aagcggctgc
     3481 ggctgtccgt aaaatcacga ttgaagcaaa ccagaccgct gattttgaag ataatgactt
     3541 cagcggcaaa cgctccctga tggagtctgt cgaagcgaag accaaagaca ttatgccagt
     3601 ggcatttgaa tttaaatgcg ttccgtttga aggtctgaaa gaacgtccgt ttaaattacg
     3661 cctcagtatt atcactggcg atcgtcctgt actggttctg cgcattattc agctggaggc
     3721 ggtgcaggaa gaaatggcta acgaatttcg tgatctgctt gttgagaaat tcaaggacag
     3781 caaagtagaa acctttattg gtactttcac cgcctgattt cattactgca aatgcccctg
     3841 cgggggcatt tatggaaacg taatttactc aataatcgca ggatggtgag ggattctttt
     3901 taccagaatt cagcgcggtg cagcgcatat acgtggagaa caaaatgtca tttattaaaa
     3961 ctttttccgg gaagcatttt tattatgaca ggataaataa agacgacatc gatattaacg
     4021 atatcgcggt ttccctttca aatatctgtc gctttgccgg tcatctttcg cacttctaca
     4081 gcgtcgccca acatgcggt
//
LOCUS       DAMZET010000270         4072 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.270, whole genome shotgun sequence.
ACCESSION   DAMZET010000270 DAMZET010000000
VERSION     DAMZET010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.270"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..460
                     /locus_tag="POB34_004609"
     CDS             <1..460
                     /locus_tag="POB34_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HDI5921432.1"
                     /translation="NPHIAITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAG
                     CFAAKVPLVSGAAIRMEGQITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGV
                     IGSLQAMEAIKLLAGYGKPASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(536..1198)
                     /gene="fsa"
                     /locus_tag="POB34_004610"
     CDS             complement(536..1198)
                     /gene="fsa"
                     /locus_tag="POB34_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDI5921433.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            1329..2228
                     /locus_tag="POB34_004611"
     CDS             1329..2228
                     /locus_tag="POB34_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDI5921434.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            2234..>4072
                     /locus_tag="POB34_004612"
     CDS             2234..>4072
                     /locus_tag="POB34_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HDI5921435.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALACEMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLIN"
BASE COUNT          989 a         1097 c         1077 g          909 t
ORIGIN      
        1 caacccacat atcgcgatta cgccagtcaa tgcactgctt gatgacgctg aacttgctgc
       61 gatgattgca aaacacgatc tggtactcga ctgtacggat aacgttgcgg tgcgtaatca
      121 actgaacgct ggctgttttg ccgcgaaggt gccgctggtt tccggcgcgg caattcgtat
      181 ggaagggcaa atcacggtct tcacttatca ggacggcgaa ccgtgctatc gctgccttag
      241 ccgtttgttt ggcgagaacg cattaacctg cgtagaagca ggcgtgatgg caccgttgat
      301 cggcgtaatt ggttcgttgc aggcaatgga agcgatcaaa ctgctggcag gttatggcaa
      361 accggccagc gggaaaatcg tcatgtacga tgcgatgacc tgtcagtttc gcgaaatgaa
      421 actgatgcgc aatccggggt gtgaggtgtg cgggcagtaa ttgccacctc caggccagat
      481 aagacgcggc actccgccac gtcatcagca atttatgagg atgcagaacg tccggttaaa
      541 tcgacgttct gccaaacgct cccagccagt cctgttcaaa cttcgccaca gcggcttcca
      601 ccgccggata gctaatcatc tgttgtgcca catccagtgg cagcgtaatt gattcacatc
      661 ctgccagtaa gcagtccagc gcctgacgcg gggttttgaa actcgctgcc agcactttcg
      721 cctgcggcgc atgcattttc aataactggt gtaagtcggt cacagtctga atgccgctac
      781 cgccctgagc atcaatacga ttaacgtaag gcgcaacata ttccgcacct gccagcgccg
      841 acagcagccc ttgtgctgcg ccatataccg cggttcccag cgttggaatc ccttccgctt
      901 ttaacatctt aatagctgcc agcccctcgg cggtcaccgg aactttcacc acgatatccg
      961 caataataga acgcaattta cgtgcgtcat tcaccatccc ttcagcagtg gtagccatta
     1021 cctgggcaaa cagacgcccc tgaccgccca tcgcttcatg aagttgcgga agcacaacat
     1081 ccagcggttt tttacccgcg gcgataatgc ttgggttagt ggtcacaccc gccagcggaa
     1141 aaatacgtga cagcgccttc accgcaacaa cgtctgaagt atccagatac agttccataa
     1201 ccatcctcaa aatgtacttt ttgtctgaat gtagactatc acgggaaaac tctctgaaca
     1261 gttgacgcac atcaatctaa ctttcattcg aaagtatttt aatgtttgaa cgaaacagag
     1321 aggcaactat gattttcaat attcagcgct actcgaccca tgatggcccc ggtatccgca
     1381 cggtcgtatt tcttaaaggc tgttcgctgg gctgccgctg gtgtcagaac ccggaaagcc
     1441 gcgcccgcac gcaggatctg ctgtatgacg cacgactgtg tctggaaggc tgcgagctgt
     1501 gcgctaaggc cgcgccggaa gtgattgagc gcgcgctgaa tggtttgctt attcatcggg
     1561 aaaagttaac cccggagcat ctgacggcgt taaccgactg ctgtccgaca caggcattaa
     1621 ccgtgtgtgg tgaagtgaaa agcgttgagg agatcatgac gaccgttctg cgcgataaac
     1681 cgttttacga tcgcagcggc ggcggtttaa cgctttcggg tggtgagccc tttatgcagc
     1741 cggaaatggc gatggcgcta ctgcaagcca gccacgaggc aggcattcat actgcggtag
     1801 aaacctgtct gcatgtgccg tggaaatata tcgccccttc tctgccctat atcgatctgt
     1861 ttcttgccga tttaaaacac gttgccgacg cgccgtttaa acagtggacc gacggtaacg
     1921 ccgccagagt gctggataac ctgaaaaaac tcgccgcagc gggcaaaaaa atcattatcc
     1981 gcgtgccgct gattcagggc tttaatgccg acgaaacctc tgtaaaagcc attaccgatt
     2041 ttgccgccga cgagctgcac gttggcgaaa ttcattttct gccctaccac acgctgggca
     2101 tcaacaaata tcacttactt aatctgccct atgacgcccc ggaaaaaccg cttgatgcgc
     2161 cagaactgct cgactttgcc cagcagtatg cctgccagaa agggttaacc gcgaccttac
     2221 gaggataaca accatgacca cactgaaact ggacacgctc agcgaccgca ttaaagcgca
     2281 caaaaatgcg ctggtgcata ttgtgaaacc gccagtctgt accgagcgcg cgcagcacta
     2341 taccgagatg tatcaacaac atctcgataa gccgatcccg gtacgtcgcg cgctggcact
     2401 ggcgcatcac ctggcgaatc gcaccatctg gatcaaacac gatgagttga tcattggcaa
     2461 ccaggcaagc gaagttcgcg ccgcgccgat cttcccggaa tatactgtct cgtggatcga
     2521 aaaagagatt gatgatctgg cagatcgtcc cggtgctggc tttgcggtga gcgaagagaa
     2581 caaacgcgtt ctgcatgaag tgtgcccgtg gtggcgcggt cagaccgtac aggatcgctg
     2641 ctacggcatg tttaccgatg agcaaaaagg tctgctggcg accggaatca ttaaagcgga
     2701 aggcaatatg acctccggcg atgcgcacct ggcggtgaat ttcccgctgc tgctggaaaa
     2761 aggtcttgat ggtctgcgtg agaaagtagc ggaacgtcgc tcgcgcatca atctgacggt
     2821 gctggaagat ttacatggcg aacaattcct gaaagcgatt gatatcgtgc tggtggcagt
     2881 cagtgaacac attgaacgtt tcgctgccct ggcgtgtgaa atggctgcta ccgaaactcg
     2941 cgaaagccgt cgcgatgaac tgctgacgat ggcagaaaac tgcgatctta tcgcccacca
     3001 gccgccgcag actttctggc aggcgctgca actgtgttac ttcatccaat tgattttgca
     3061 gatcgaatct aacggtcact cggtatcgtt tggtcgtatg gaccagtatc tctacccgta
     3121 ctatcgccgc gacgttgaac tcaaccagac gctggatcgc gaacacgcca tcgagatgct
     3181 gcatagctgc tggctgaaac tgctggaagt gaacaagatc cgctccggct cacactcaaa
     3241 agcctctgcg ggaagtccgc tgtatcagaa cgttaccatt ggcggacaaa atctggttga
     3301 tggtcaacca atggatgcag tgaatcccct ctcttacgcg atcctcgaat cctgcggtcg
     3361 cctgcgttcc actcagccta acctcagcgt gcgttaccat gcaggaatga gtaatgattt
     3421 cctcgacgcc tgcgtacagg tgatccgttg cggcttcggg atgccggcgt tcaacaacga
     3481 cgaaatcgtg atcccggaat ttattaaact cggtattgaa ccgcaggacg cttacgacta
     3541 cgcagcgatt ggttgtatcg aaaccgccgt cggtggcaaa tggggctatc gctgtaccgg
     3601 catgagcttt atcaacttcg cccgcgtgat gctggcggcg ctggaaggcg ggcgtgatgc
     3661 caccagcggc aaagtgttcc tgccacaaga aaaagcgttg tcggcaggca actttaacaa
     3721 tttcgatgaa gtaatggacg cgtgggatac gcaaatccgt tattacacgc gtaaatcaat
     3781 cgaaatcgaa tatgtcgtcg acaccatgct ggaagagaac gtacacgata ttctctgctc
     3841 ggcgctggtg gatgactgta ttgagcgagc gaaaagtatc aagcaaggcg gcgcgaaata
     3901 tgactgggtt tctggcctgc aggttggcat tgccaacctc ggcaacagcc tggcggcagt
     3961 gaagaaactg gtatttgaac agggcgcgat tggtcagcaa cagctggctg ccgcactggc
     4021 ggatgacttc gatggcctga ctcatgagca actgcgtcag cggctgatta ac
//
LOCUS       DAMZET010000271         4038 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.271, whole genome shotgun sequence.
ACCESSION   DAMZET010000271 DAMZET010000000
VERSION     DAMZET010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.271"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..2195)
                     /gene="pat"
                     /locus_tag="POB34_004613"
     CDS             complement(<1..2195)
                     /gene="pat"
                     /locus_tag="POB34_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HDI5921436.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPI"
     gene            complement(2227..2925)
                     /gene="tapT"
                     /locus_tag="POB34_004614"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(2227..2925)
                     /gene="tapT"
                     /locus_tag="POB34_004614"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HDI5921437.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(2994..3413)
                     /gene="trxC"
                     /locus_tag="POB34_004615"
     CDS             complement(2994..3413)
                     /gene="trxC"
                     /locus_tag="POB34_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HDI5921438.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            3620..>4038
                     /locus_tag="POB34_004616"
     CDS             3620..>4038
                     /locus_tag="POB34_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase YfiF"
                     /protein_id="HDI5921439.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPR"
BASE COUNT          961 a         1073 c         1105 g          899 t
ORIGIN      
        1 aaaatcgggc ggaacaagca gcgttcaccg tttttcaatt ctacccattc ttccagctga
       61 tgcggataag ggcgcactgc cagccgactc tcgttatcgc cttcaaacgg cgagatatcc
      121 agcgtgacat ccagcgcggt aaattcactg ccagaagcca gcaaaggatg aatatccaga
      181 cgctgaattt ccgggcaatc gacaatcaag ttggaaacct gcaccagaag ctggctcaag
      241 cctgcaacat ccaatgggcg tagcgcactg cgtgcacgaa tctttttact tttgatcccc
      301 tgaataacca gatagcgggc caggttcatg ttcagcggcg gtaacgcgac gacggcttga
      361 tcttcaggac gccactccac accgccttcg cccagcatga tcaacggccc gaaaaccgga
      421 tcgtgctcaa ccacaacccg caactcctga gcaccagcac ggttagccat actttgcacc
      481 aacaggccgt ggacccgtgc ctgcggccag gccattttta cgcgatcgaa aatggcgttt
      541 gccgcttgct ggacttcatt ggctgtacgc aggtagagca tgacgccctg aacttccgat
      601 ttatgtggaa tatccggcga acgcaatttc agcgccaccg gataaccaat ctgttcggca
      661 atatgcaccg cttcggtgct atcgctggca atccaggtgg ggagcgtgtt catgccatat
      721 gcttgcagga taggctgaac ttcatgggta tcgagtgacg ttgctccttc ggcaatcgcc
      781 tgttgcaaca gaagatgcgc ttctgcggta ttggaggtca gattgctggg caacgccggc
      841 gtttcgcgta gttgcttctg attacgccgg tactccacca tatgcataaa agcagtgatg
      901 gttccttccg gggtacggta ggtcggcagc ccggcttcgc tgaataatcg tcgtgcctct
      961 tgcgaggaat gttcgccaca ccagttcgtc agcagagaaa catacttgct gcggggatga
     1021 tgctttaccg cttcaattaa tacttgcgcg ctttctgttg cgggagcagc ggcgctgggc
     1081 gaatgaataa ccatcagcgc atcaaaatcc tggctgtgga gcagaatgtc cagcgtttta
     1141 atatagtgct cactgctggc gtcatcgcgc agatcgagcg ggttagagat tgccacatgt
     1201 cctggcagtg catcgcgcag tttctggcag gtttcttcgc ttagcgttgc cagcttgcca
     1261 ttgcgtgacc ataaggcatc cagcgccagc gcggcaggcg cagcaccgtt gctgataatc
     1321 atcaaccggt cgccacgcag cgggcgcata tggctaaggg tttccaccgc cgaaaacagt
     1381 tcgtgggtat cctgtacccg caacaaaccg gcacgctgaa tagccgcatc ccatgccggg
     1441 tccattcctg ccgtcgtgtt gagcagtcgc tgtgccgccg ggctacgtcc gcttttaatc
     1501 accagaatcg gtttattacg cgaggcacta cgggctgccg aaacaaagcg tcgcgcgtcg
     1561 cttaactgtt cgagatagag caggatggcg ctggttttac tgtcgcgcgc cagatagtcg
     1621 agcaattcat caacgtcgat atccaggctg tcgccgagcg caataaagta ggaaaagccc
     1681 atctcacgct gttgcgccca gtcgaggatg gtgttggaga cggctgccga ttgtgaaata
     1741 aacgccagct tgccgcgttt aatcggcaca ggcgaaaagc tggcattcag accttgccag
     1801 ggagccagta aacccagact gtttggtcca agcaggcgca tgttatggcg cagggcgcag
     1861 gcgcggagat cttcgtgttg cgatgccggg gcggaaagaa taatgcaggt tttacagcct
     1921 ttctcgccga gctcttccag aagagcaaga ttacggctgg cattggtaca taaaaccgca
     1981 aggtcgggtg taaagggcaa gctggcaata tccggccagg ccaacacacc caacaccgct
     2041 ttccaggctg gcgtcaccgg gagtaccggt ccgttaaagc ctcccgccag caggttacgc
     2101 atcatcaggt aacctgcgcg attgggtttc atcgacgcgc caattaccgc tatcgatttt
     2161 ggtcgcagta gtgcttccag tcctcgctga ctcataccgg tctcccgcag aagtgaccga
     2221 atgattttaa acgctttcta actgttctgc tgtgatgcta cccagatgtt gcgtttttcc
     2281 tgccagatag cgtgttttaa agcgggtaaa atgctcgcct aaccctgctg ccgccccggt
     2341 atcgccggcc atatctaaca gtgcgatggc tacctcggca gtacaatatt ggccttcagc
     2401 ctgggcttca cgcaggcgat aggcagaaag ccgggaaaga tcgacggaaa tgacgggaag
     2461 attatccaga tacggacttt tacgaaacat cttgcgagct tccggccagg taccatcgag
     2521 catgataaac agcggtggct taccggcagg tggtgtgaag atcacttccc gttgctcatc
     2581 agcatacgaa gcgggaaaga ccaccattgg ctgatagtcc gggttttgta ccagatccag
     2641 taaatcttgc gagggttcgg tacgcgacca ttgaaacgca acggtatcag gcaaaatatc
     2701 agcaatcaga cgcccggtat tactgggctt cattggctcg gtgtcgaaca tcagcaaaca
     2761 gaagcgactt tttgcttgtg ctggggtaat tgtcgaacag agacataatt tctctggcaa
     2821 aagacagcgt tggcagcgac gaacgcgatt accgcgggca agaaaaggac gtgttgcgcg
     2881 cgcaatacgc tcggcgcgta actggagaac agcgttttcg gtcataagag agcgtcgaaa
     2941 aaacgccatt gtcgcagagg agaaaacggg gcacaagatg cgccccggta agattaaaga
     3001 gattcgttca gccagctatc gaacggcgct ttcggtactg cgccattaag catgtcgaca
     3061 acctgaccgt ttttgaaaat catgatcgtc ggtatactac gaattccaaa gcgactgctc
     3121 aattcacgtt cagcttcggt attcactttc acaaagcgca ctttaccgct acgctcttgc
     3181 gcgacatctt caaaaattgg tgcgaaatta cggcaggggc cgcaccacgg tgcccagaag
     3241 tcgatcacca caggtagatc atccttcagc aatttgtcga gtgtttcacc ggtcgcatta
     3301 atcacctctc cgtcaaacaa gtcgtgaccg cagcgtccgc attttgccgc atcttcgatc
     3361 cgatcgtcgg gaatgcgatt gatggcctga caatgggtac aaacggtatt cataactaac
     3421 ctcgggatga gtaaggagac ttatcggcaa cgacgccggt atgtttctaa tatgttacat
     3481 attatcgggg acattgttcc agacaacaat ctgttttatt caataggtat gatagtcgca
     3541 ggctttggta cgaaatcatg gcgaaccgcg cggacttcgg gtaatctgcg cgcttcgcgc
     3601 agcgctggtg gagaaaagca tgaacgatga aatgaaaggt aaaagcggca aggtcaaagt
     3661 gatgtatgtc cgcagtgatg atgattctga taaacgtacc cacaacccgc gtaccgggaa
     3721 agggggcggg cgtccaggaa aatctcgtgc tgacggtggc cgtcgccccg cccgcgatga
     3781 caaacagagt cagccccgtg accgcaagtg ggaagattcg ccgtggcgca cggtttcccg
     3841 cgcgccgggt gatgagacgc cggaaaaggc cgatcacggt ggcatcagtg gtaaaagttt
     3901 tattgatccg gaagtgttgc gtcgtcagcg tgcggaagaa acccgcgtct acggcgaaaa
     3961 tgcatgtcag gcacttttcc agagccgtcc ggaagcgatt gttcgcgcct ggtttatcca
     4021 gagtgtaacg ccgcgttt
//
LOCUS       DAMZET010000272         3928 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.272, whole genome shotgun sequence.
ACCESSION   DAMZET010000272 DAMZET010000000
VERSION     DAMZET010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.272"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..230
                     /locus_tag="POB34_004617"
     CDS             <1..230
                     /locus_tag="POB34_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HDI5921440.1"
                     /translation="FCGAEGLNNVITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVV
                     IDENDNVIFSQLVDEITTEPDYEAALAVLKA"
     gene            complement(274..1815)
                     /gene="tyrR"
                     /locus_tag="POB34_004618"
     CDS             complement(274..1815)
                     /gene="tyrR"
                     /locus_tag="POB34_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HDI5921441.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(1963..3024)
                     /locus_tag="POB34_004619"
     CDS             complement(1963..3024)
                     /locus_tag="POB34_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HDI5921442.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(3021..>3928)
                     /locus_tag="POB34_004620"
     CDS             complement(3021..>3928)
                     /locus_tag="POB34_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HDI5921443.1"
                     /translation="GEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGL
                     HFIQPGRFVLPGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEK
                     VVKGFYKNHFLRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRL
                     FSPVIDKLLFAATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQA
                     TTSGIIDVNGEKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQV
                     MDVDKPLPHIRLDAALEFLIGDKLR"
BASE COUNT          941 a         1136 c          953 g          898 t
ORIGIN      
        1 gtttctgcgg cgcagaaggt ctgaacaacg ttatcaccct ctccactttc cgtaacgctg
       61 aatttctgca agcttacggt gtggcaattg ctgatggccc actgaaaggt ctggcagcgc
      121 gtgccgttgt ggttattgac gaaaatgaca atgtgatttt cagccagctg gtggatgaaa
      181 tcaccaccga gccggattac gaagcagctc tggctgtact gaaagcataa tttaatatgc
      241 ctgatggtgt tgcaccatca ggcatattcg cgcttactct tcgttcttct tctgactcag
      301 accatattcc cgcaacttat tggcaatcgc ggtatgtgaa acgccgagac gttttgccag
      361 tttgcgcgtg ctgggataat tgcgataaag ctgggttaat accgagcgtt caaaacggct
      421 ggtgatttcg tccagcgaac cttccatcgc atcttcgccc acggctaccg ttgcggcgtc
      481 ataatccggc aacaaaatat cctgtggacg cagctcataa ccgtccagtt gtgtcagtgc
      541 gcgatagata gcgttcttta actgccgcac atttcccggc cacgcataac gcgtaagtac
      601 agtattcagg tcagcggcca gtttcggacg cggcacgccc tgctcgtcgg caaagcgggc
      661 gacgaacagc tcagttaacg gcatgatgtc ctgcggacag tcacgtagcg gcggcagatt
      721 gagcgtcaac acgttcagac gataatagag atcttcacgg aacatgcctt tttgcaccag
      781 ttcgaccaga ttcttctgcg tagcgcaaat cacccgcaca tcgacatgca cctcatggtc
      841 ttcgccaacc cgacggaaag tgccatcatt aaggaaacgc agtaatttcg cctgcatccg
      901 tggtgacatt tcccctattt catccaacag caccgaacca ccgttcgcct gctcaaagaa
      961 tcctttcttc ccttccggag catgaccaaa cagttcactc tcgaccgcat cttccggtat
     1021 agacgcacag ttcagcgcca ggtaaggttt gcccgctctg gggcttgcct gatggcaggc
     1081 gtaggcaaag agatctttac ctgtacctgt gtcacccgta atcagcagcg gcgcgcttag
     1141 catcgccagt ttctgcgcct gttcgacaac atgcttcatt ttcgggctga cggcgacaat
     1201 ttgactgaag gcgctgacgt cctgggcggc gacattttgc aactggcggc ccatacgaat
     1261 cgttgatcgc aacatcacca ccgcaccggt caggacgtgt tgatcatttt catcctgaag
     1321 ataaacaggc gtaatctcca tcaggaaatt ctgcccatta ataacgacat gctcgttatg
     1381 cgaatcttgc ggttcgcttt ccagccaacg taaaaaatta aagccgttaa tcaattgtgc
     1441 ggcggtatgg ttgcgcaggc gatccaattt ttgcccaaaa agctgacagc tcgccgggtt
     1501 cgccatatcc actttgcttt tcatatcgac agagagcaca ggttcaggca acgcctccag
     1561 taacgcgctc aacgccagat gctcacgttc ggaaggcatc cacgggacag tacgcacatc
     1621 ggtaacaccc gcaatacggc gtatttcggc catcagactg ctgaaactct caaactccag
     1681 ttcagcaaaa ttgaggtaga ttcgcccaat gggatcaatc tcaataccgc gtaaatcaat
     1741 gcctcttagc acgagtagat cgagtaattc gcgggtcaga ccgagtcggt cttcacaaaa
     1801 gacttccaga cgcatgggaa ccttcacctg aaaaaagaac aattaatatg atgatatgac
     1861 actatttgga tagcaggaag gtttctgtca acaatcattg acacaaagac ggaaaaacgg
     1921 gcacttcggc gtaaagatat ccggtcagca cccgatattg cattattttt cgctgggcgt
     1981 tttgcctttt tgcagcgttt ctttcacctg accgataagc tgacgacgga aatccccgag
     2041 gcgaggtttg tcatcgtcaa tccacggcag cgggcggcaa agctccatag ctttaatccc
     2101 gagtcgtgcc gtcagaagtc ctgcaccaat cccctgagct gcgcgggtag acaaacgagc
     2161 agcgagatct tgcgacatcc agtccatccc cacttcgcgc accagttcgc tggctccggc
     2221 aaaagcgata ttcagcaata ccagcttaaa cagacgcaaa cggctgtaat accccagttc
     2281 aatgccatac agcgtggcga tgcgattaat taaacgcaga ttgcgccagg cgataaacgc
     2341 catatcgacc aacgccagcg ggctgaccgc aatcatcaac gttgattccg ccgccgaacg
     2401 gctgatttcg cgccgcgcct gggcatctaa aactggctgg accaaatgcg catacaggct
     2461 gaccacttca cggtcgtttt gcgtttcatg gattgaggca taccagcgtt gcagcgccgg
     2521 atgcgactga tcaatacccg cctgctgcgc cagtttttcg caaaatgcgc ggcctttgcc
     2581 cgtgccgtgg ctatgcaata aatcacgcgc ttcgtcgcgt tcatgggcgc gctgtcgcaa
     2641 gcgccataag cgccgccact ctgttaccac agaacctacg ccagcgccga taatcaatgc
     2701 cccagcggca catccaccca gcgccaccca gtcctgggtt tgccaggcat tcattgtcca
     2761 ctgtacaccc tgcccgacaa cgcttgcgcc aaacagagcc agcccgccca tcaccatttt
     2821 gcgccacagg ctgcgtttcg gacgtaacgc tgcgtccatt accgcttcga cttgcccctc
     2881 ttcttcctgc gcttcgtcga gcgtggccgg ggcaaaattt tgcgcctgat tttcgtcaaa
     2941 ggtctgctgc gccctgaatt taggattctg atcgacctcc agaggaccgt cgaaatcaat
     3001 acgtggtttt aacggttcgg tcatcgcaat ttatctccta ttaaaaattc cagcgcagca
     3061 tcaagacgaa tatgcggtag tggtttgtcg acatccatca cctgcggacg aaatgcctca
     3121 aactggaagc cttgcttatc ccagaacgcc tgaccaggca aacgtgcggg aacttcgcca
     3181 ggataaacag tgagcggtgc gccatcgcta agtcgattac cacgcagcgc cgggattttc
     3241 tcaccgttaa catcaataat gccgctggtg gtcgcctgaa ctgacgccag ccccaggcag
     3301 tccatgctga tcccttcgaa cgccgcattt tgccaggcat cctgaatcag ttgttgcagt
     3361 aatgaaacca tattagcgtg ctgatcgatg gtcacatggt ccgctttagt ggcagcaaac
     3421 aatagcttat cgataaccgg cgaaaacaaa cgccggaaca gggtacgctg cccgtagtga
     3481 aaactttgca tcagctgcgt cagtgccaga cgcatatcat taaatgcctg tggcccactg
     3541 ttgagaggtt gcaggcaatc caccagcaca atctggcggt caaagcgcag aaaatgattc
     3601 ttatagaacc ccttcaccac cttctcgcag taataattaa agcgctcgcg cagcattccg
     3661 gcattggtat gcttatcggc ctgcgccagt ttggactcgc cccaggtatc gacatccggc
     3721 cacgggaaga attgcagcgc gggcgcacct gccatatctc ccggcaagac aaagcgccca
     3781 ggctgaataa agtgcagccc ctgctgttta cagtggtgga gataatcggt ccacgcggcg
     3841 gcaatgtccg ccagccggtt ttcgtcggca ggcgctagcg ggtcaagtcc ttcgcacatc
     3901 attcgccatt tcgccgacca ttcgccgc
//
LOCUS       DAMZET010000273         3917 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.273, whole genome shotgun sequence.
ACCESSION   DAMZET010000273 DAMZET010000000
VERSION     DAMZET010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.273"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..219
                     /locus_tag="POB34_004621"
     CDS             <1..219
                     /locus_tag="POB34_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HDI5921444.1"
                     /translation="ISAERLQEKVGREILVIIDEVDEEGAIGRSMADAPEIDGAVYLN
                     GETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(266..1594)
                     /locus_tag="POB34_004622"
     CDS             complement(266..1594)
                     /locus_tag="POB34_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDI5921445.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYYAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTQEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(1602..>3917)
                     /locus_tag="POB34_004623"
     CDS             complement(1602..>3917)
                     /locus_tag="POB34_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDI5921446.1"
                     /translation="LIILFLLAALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVG
                     YFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRR
                     ANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPG
                     KKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKN
                     QLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPGGNLLNASDKLF
                     YLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIEALKIREGDAKE
                     IISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCG
                     LEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIA
                     MVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDA
                     KLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVSAERGIISP
                     LKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFH
                     ERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSN
                     LKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVAREL
                     IKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         1142 a          916 c          774 g         1085 t
ORIGIN      
        1 atttccgccg agcgcctgca agagaaagtg ggccgtgaaa ttctggtgat tatcgacgaa
       61 gtggacgaag aaggcgcgat tggtcgcagc atggcagatg caccggaaat cgacggcgcg
      121 gtttatctca atggtgaaac caacgttaag ccgggtgata tcctgcgtgt gaaagtcgag
      181 cacgccgatg agtacgattt gtggggtagc cgggtttaat taattcaggc cgggcaaaat
      241 catttgtccg gcctgcaaat aatgattact gccagctaat ctgagccctg ccgtttttct
      301 tactcttata cagccgctca tccgccagca ttttcaagcg ttcgacggtg gtacattcat
      361 ttgaatgcgc tacaccgata ctggtactta aattaatgct tcccacctgg aaatgaacga
      421 tttcttttag aatcgatagc aactcttcct gcgttccacc cgtataaatc accgcaaatt
      481 cgtcaccgcc gaagcggtag attttacctt tatctaccgg gatctgtgat ttaaggattt
      541 tcaccacttt catcaacact tcatcgccca gcaaatgacc gaaggtgtcg ttaatgtttt
      601 tgaatttatc gacgtcaaac aggcacaaat aaccgccttt ttccggcagc gcttttaaat
      661 cttcgtcaaa ggattttctg cccattgctt tggtcaggtc gtccaggaac gccgagttat
      721 acatactgta gagttttttg acatcggcag ccgaaaagaa aagcccgcca tccagtacgc
      781 gttcaaaatg gttattaatg atggcttctt tctccacaat accacgatta atcactctgc
      841 gtaccagata gaggcaactg gtacccgata tcactgccac caatgcggca aagaaaataa
      901 tcaatgtaca gacccgcaaa taaacatctt ttttatacgc aaatacgtac aatccggtcg
      961 gcaaataggc gaagtgctcc atcgcttccg aaacggtgaa gatggcgttt ttagcataat
     1021 attcatcacc ggtcaccggt ttaccgttct taaatagaga gaacatcagg ttctcgctgt
     1081 ccatcgccag cgattttgac agtaaagaaa aacgctccag accaagcaaa aatatcagac
     1141 tacgattgtg agttaccgga tagaggtaat agacgaaggt tttaccgtca aaagagataa
     1201 accctgtatt tttgacatgt cgatacttca caacctgcat aatatctgca caataatttt
     1261 tctcgaactc ctttacctga atacattcgt cactggaaat cagataagga ccgacatcgt
     1321 acaaataaga gggggaaacc gagtcggcgt aagctgtgcg caaaccaacc aacagctgtt
     1381 cctccgtggg atttagcgtg ttgataagat caattgccac gctacgccga tattcagcaa
     1441 tatttgccac cgcaaaagca gtatcaataa cggactgttt caggctatac acccgttcct
     1501 ttaccatttg agtgtagatc ccgcaggcaa ctgctgaaat catgataaaa atcagcagac
     1561 tgactgtaag tacgaactta ttgattctgg acatacgtaa actactcttt tactaatttt
     1621 ccacttttat cccaggcgga gaatggcatg ggtttctggt acagataccc ctgagcctga
     1681 acacatccta atttaattaa ttcgcgcgca acgtcttcgc tctctacgcc ttccgcaatc
     1741 accgggatcc ggttatagcg tgccagatcg aaaataatgc gcacaatttt tcccgcagta
     1801 ccgaaaatat cgccggtaaa ttgcgagtcc acctttatgc tgtctggatt aatttcataa
     1861 aaacgtttca ggtttgacaa accggtgcca aagtcatcaa tcgatatccg taccccttgt
     1921 ttacgcagtt gttcaatatt gagataaaaa tgttcattaa ttcgctcgat cacggtttca
     1981 gtgatctcaa cacagaggct gttcgccttc aggccatttt ctttcatgat ggtggtaaaa
     2041 cgctcatgaa agtgcggttc gtttaactgc cagggcgaaa cattaatatg cagtaagaaa
     2101 tcatccttca gcgacgcacg ctgactaaaa tgtctgaatt cacccattgc cgttttaata
     2161 atctgataac ccagctcatt gataaacccg atatcttcag caatggtaat gaacttcagc
     2221 ggtgaaatga tcccccgctc cgcagaaacc caacggcaca acgcctccgc gcccagaatg
     2281 gcgcgggtgt taatatcaac gatcggttga tagacgagga atagttcgcc aatttccatt
     2341 gcatggcgaa tattacgcgc aatccagata ttctcttcta tttcgttgag tttcgcgtca
     2401 tcacaaaaaa taaactcgga gaacatatgg tcacggatac tggaaatcgc cagtttcagt
     2461 ttttctgcgt aactccaggc gttttccccc tgcaaaggtt cacaaatggc agacttatgg
     2521 atgcgcagat tgcggtagcc aaagctgctg tcaaaatcgt ttaccatcgc tattttacgg
     2581 gtaaatgtct gtacattttc ccgacacaga agtaaataga gatcggcatt gaatgtcacg
     2641 atatcgcagc attgcgcata tttctcggca attcgctgac acaatttcct gacgatttta
     2701 ttgcgttcat caacaccaaa caccgcttcc acggcttcca gaccacacag tttgatgatc
     2761 cccacagcca gatactgatt attgttggac tcaataatcg ccatattttt aataaaaccg
     2821 ctttcctgat cgtaagagat cagtgagagc aatttatttg accgttcaat ttcggcatct
     2881 ttcatttccg cgatcgaatt tttgatgctg ataatctctt tcgcatcgcc ttcgcgaatt
     2941 ttcagagcct cgatctgatc cgttgagtcg ggcaaaaacg ccagcgtatt aaccagctcg
     3001 gtcatttcag aaaattgccg acggaacaca cgagtggtca tgaaatatac caccagcaac
     3061 acgataataa tgaacgcaaa cggtagataa aaaagtttat ctgatgcgtt caataaatta
     3121 ccgccaggat aataaaaata gatttcgaac aatgcattat ttggcactgc tgatgtcttt
     3181 gcaatatact ccgtatcgac agaatcaata atattataat taccttcgct attataaata
     3241 atcagttttt cacgagcgag ttgattcttg gcaatgatct catgaccttg tttcaaaaat
     3301 agaacttcat cattagcgct gatttgatca agatactcgt tcagataccg ccagtttaaa
     3361 tcgacatgaa ttttatctac cgctaacaaa tcgtgatctt tgccgttgat acgtagcgta
     3421 aaagagatac attttttacc cggacgatca aagcaattgt atgccggatt ccagctgttg
     3481 ttagagtcat ctgaagccac cgaccacggt cgtttcgatg ggttaaaatc ggcaaaaata
     3541 acccgccctg actcgtcgac aagcggtcca tttacttgtt cctgatgata aacaacgatt
     3601 ttatcattcg ccctccgcga aaagagataa tatttattat catcaagtac cagtcccacc
     3661 gagtctaaaa atggcatcat ttccattttc agaatcacgg cgcgttgaat ctcctctgta
     3721 ttttttaata aatacagtga gtcctttaat gtcagggccg aacgttcaat atctttaaag
     3781 aaatagccaa cgtttttctg aataagttca aagcggctat cggcacgttt taccgcgcgt
     3841 tccgatacca gttgatagtt aatgatgaaa aaaagaccaa taaatgacag tgctgccaat
     3901 aaaaataaaa ttatcag
//
LOCUS       DAMZET010000274         3868 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.274, whole genome shotgun sequence.
ACCESSION   DAMZET010000274 DAMZET010000000
VERSION     DAMZET010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.274"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            313..1611
                     /gene="kgtP"
                     /locus_tag="POB34_004624"
     CDS             313..1611
                     /gene="kgtP"
                     /locus_tag="POB34_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HDI5921447.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1608..1931)
                     /locus_tag="POB34_004625"
     CDS             complement(1608..1931)
                     /locus_tag="POB34_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HDI5921448.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="POB34_004626"
     CDS             complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="POB34_004626"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HDI5921449.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3446..>3868)
                     /locus_tag="POB34_004627"
     CDS             complement(3446..>3868)
                     /locus_tag="POB34_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5921450.1"
                     /translation="TKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQT
                     EEILGVTRAISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITM
                     PNNRGMVALARKLGFNVDIQLEEGIVGLTLNLAQREES"
BASE COUNT          941 a          945 c          938 g         1044 t
ORIGIN      
        1 aaaggccatc cgtcaggatg gcctttttgc attggcgcag aaaaaaatgc ctgatgcgac
       61 gctgcgcgtc ttatactccc acatatgcca gattcagcaa cggatgcggt ttccccaact
      121 tgcctacttc catacgtgtc ctccttacca gaaatttatc cttaagctcc tcaataacca
      181 ttttcctgct aactaaattc atggttaagg ttgcataatg atacgcaaca aatgtataac
      241 attttcttta ccaaaagaaa taaataaaag cgaccgacaa aagcatcgga ttacggcagg
      301 agacataatg gcatggctga aagtactgta acggcagaca gcaaactgac aagtagtgat
      361 actcgtcgcc gcatttgggc gattgtgggg gcctcttcag gtaatctggt cgagtggttc
      421 gatttctatg tctactcgtt ctgttcactc tacttcgccc acattttctt cccttccggg
      481 aacacgacga ctcaactact acaaacagca ggtgtttttg ctgcgggatt cctgatgcgc
      541 ccaataggcg gttggctatt tggccgcata gccgataaac atggtcgcaa aaaatcgatg
      601 ctgttatcgg tgtgtatgat gtgtttcgga tcgctggtta tcgcctgcct cccaggttat
      661 gaaactatag gtacgtgggc tccggcatta ttgcttctcg ctcgtttatt tcagggatta
      721 tccgttggcg gagaatatgg caccagcgcc acctatatga gtgaagttgc cgttgaaggg
      781 cgcaaaggtt tttacgcatc atttcagtat gtgacgttga tcggcggaca actgctagcc
      841 ctactggttg tcgtggtttt acaacacacc atggaagacg ctgcactcag agagtgggga
      901 tggcgtattc ctttcgcgtt aggagctgtg ttagctgttg tggcgttgtg gttacgtcgt
      961 caattagatg aaacttcgca acaagaaacg cgcgctttaa aagaagctgg atctctgaaa
     1021 ggattatggc gcaatcgccg tgcattcatc atggttctcg gttttaccgc tgcgggctcc
     1081 ctttgtttct ataccttcac cacttatatg cagaagtatc tggtaaatac tgcgggaatg
     1141 catgccaacg tggcgagtgg cattatgact gccgcattgt ttgtattcat gcttattcaa
     1201 ccactcattg gcgcgctgtc ggataagatt ggtcgccgta cctcaatgtt atgtttcggt
     1261 tcgctggcag ccatttttac cgttcctatt ctctcagcat tgcaaaacgt ttcctcgcct
     1321 tatgccgctt ttggtctggt gatgtgtgcc ctgctgatag tgagttttta tacatcaatc
     1381 agtggaatac tgaaggctga gatgttcccg gcacaggttc gcgcattagg cgttggcctg
     1441 tcatatgcgg tcgctaatgc tatatttggt ggttcggcgg agtacgtagc gttgtcgctg
     1501 aaatcaatag gaatggaaac agccttcttc tggtatgtga ccttgatggc cgtggtggcg
     1561 tttctggttt ctttgatgct acatcgcaaa gggaagggga tgcgtcttta gtgacgggtc
     1621 agttgccaga cggtatagcc ggtgcttgca ccggcgacat cccaggccaa atccttccag
     1681 ctccagccgc tcccttcggg gcggctatcc caaagctctt ttgacgcccc caaactgaca
     1741 gagaacatca atccaaacat ggcactgcga tcccggctca tcccctgatg ctgtgaatat
     1801 tcatttccgg cggcggaaag catcgccgag gcgataaagt gttgagcttt atcctgcccg
     1861 ctccagctat cgttagccat atggctgcat ccagaaagta acaatagcga acagacaaaa
     1921 agaatacgca aaacgtggcc tccaaaaaac aaaccccgta caggacgggg ttgtgattac
     1981 aggatgcggc taattaatcg gtcgatgcgg atacggcgca aacggcggat gagtttacga
     2041 accttcaccg gataatcggc aatactttgc agatcgcgat agtgcttaac gatggtggta
     2101 tgctcgcgga tcagctccag ttctttctct cgctgcggcg caagctccag ttgtggatcg
     2161 tggatcaaaa tggcgttttc cagatccaga cgccaggcgc gcgggttcag gttattaccg
     2221 gtgatcaaca tccacttatc atcaacccac atccctttta ggtgataggt gttgtcgtca
     2281 tctttccata accgaaccac tagctggtca gtattgacgt aatactgcaa acggctcagg
     2341 aaacgacgca gattgatctc atagagataa ggcaatgcgc caattatctt gaaaggttca
     2401 tcttccggaa tgtagaagtc gttcgcggtt ttatcaccaa caataatttc gacctttttc
     2461 ccttcgcgca gcaactggat aatattgcgc acaaggattg ctggcaggtt gaagtatggc
     2521 gtacagatgg ttagtttctg ctcggcacaa ggcataagat ggaaaatggt cttgttcaac
     2581 agactcgatt tccccagccc cactagcggc gttacggaaa gctgatcgtt gtcggcatcg
     2641 ccctggaaat gataagcggc atcacgcagc tcctggcgga acagacgaat atcgttcttg
     2701 atttccgggc tttttggccg attaacatca tccagacgat taacgccgcg gccattcata
     2761 atattctgtg taacccattc aaacataatg tctgacatct tacggttacg gataagatga
     2821 taacggtcgt agcgatattt atcgtgctga tgcaggtaaa catcgttcag gctggcaccg
     2881 ctataaagta cgctatcgtc gatgataaag cctttaaagt gcagaacacc aagggcttca
     2941 cgggtattga ttggaacgcc ataaaccgga acatctacgc ccggattttc ctgcgccatg
     3001 cggcagtacc agtcagcgtt agtgttagat gccgcagcgc caatgcgtcc acgttgtgca
     3061 cgatgccagt cgaccagcac ccgcacatcc agttccggac gctgcctttt agcttcatac
     3121 aacgcgttca gaatgccttt gccaccgtca tcctgttcga gatacagggc gacaatgcaa
     3181 atgcgctgct tcgcgctggc tattttttcc agcagcgtct cccggaagtc ggcgggagcg
     3241 taaaagaaat cgacatcatc aactgattga gaaatcttgg gtagttgggc aaggtgttgt
     3301 tgatgtttat tacgcttaaa ttttgacaac atcacagtgc atttcttctc tgttcattga
     3361 agggtcctct gtgcaatgca gacgacataa gcgggcaata ataacaccag tcccgattaa
     3421 gtggtcaaca tttccagtac cttactcatg attcctcgcg ttgggcaaga tttagcgtaa
     3481 gcccaacgat cccctcttcg agctggatat caacgttaaa cccgagcttg cgggctagcg
     3541 ccaccatgcc acgattgttt ggcatcgtaa taccattcag acgttgtagt ccgtgatctc
     3601 gcgtataggt aatcaacttt tccattaagc gtcgacctaa gcctaaccct ttgagatccg
     3661 agcgaaccag cacagcaaat tcggcatcga tgttatcagg atcggaaatc gcacgcgtga
     3721 cgccgaggat ctcttccgtt tgatcaatac gtcgtaccgc tacaaacgcc atttcccgat
     3781 cgtagtcgat ctgtgtcatg ttggctaaat cttcatgggt aaattcgttg atctcgctaa
     3841 agtagcggta ataaagatct tctttggt
//
LOCUS       DAMZET010000275         3822 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.275, whole genome shotgun sequence.
ACCESSION   DAMZET010000275 DAMZET010000000
VERSION     DAMZET010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.275"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..364
                     /gene="yeeE"
                     /locus_tag="POB34_004628"
     CDS             <1..364
                     /gene="yeeE"
                     /locus_tag="POB34_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HDI5921451.1"
                     /translation="TANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            378..605
                     /locus_tag="POB34_004629"
     CDS             378..605
                     /locus_tag="POB34_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDI5921452.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(648..2075)
                     /gene="sbcB"
                     /locus_tag="POB34_004630"
     CDS             complement(648..2075)
                     /gene="sbcB"
                     /locus_tag="POB34_004630"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HDI5921453.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYVDDKEKVALLKALWQYAEEIV"
     gene            2284..3450
                     /gene="dacD"
                     /locus_tag="POB34_004631"
     CDS             2284..3450
                     /gene="dacD"
                     /locus_tag="POB34_004631"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HDI5921454.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPQIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILLRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            3569..>3822
                     /locus_tag="POB34_004632"
     CDS             3569..>3822
                     /locus_tag="POB34_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDI5921455.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAIYYDNPDETPAEKLRCDTVVTVPGYFTLPENSEGV"
BASE COUNT          946 a          885 c         1005 g          986 t
ORIGIN      
        1 aacggccaat attctgcaat ttctggtcgc gggtgacgtg aaatacatta actggggcgt
       61 tttcctggtg ttagggatct tcgtggggtc atttattgca gccaaagcga gccgtgagtt
      121 ccgcgttcgc gcagctgatg cacaaacaac attacgtagc gggttaggtg gtgtactgat
      181 gggcttcggt gccagtattg caggtggttg ctctatcggt aatggactgg ttatgactgc
      241 aatgatgacc tggcagggct ggattggcct tgtatttatg attctcggag tctggactgc
      301 gtcctggctt gtgtatgttc gaccgcagcg taaggcgcga ctggcaactg ctgcggcaaa
      361 ttaagtaagg tgtaaagatg gcgattaaaa agctggatgt ggtgacgcag gtctgtccgt
      421 ttccactcat agaagcaaaa gcagcactgg cagaaatggc cagcggcgat gagttggtaa
      481 ttgagtttga ttgcacccag gcaactgaag caatcccaca gtgggcggca gaagaaggac
      541 atgccatcac cgattatcag cagattggcg atgcagcgtg gagcatcacc gttcaaaaag
      601 cctgactcaa cattgtcctc cgccgtacca gcggcggagg cttcaaatta gacgatctct
      661 tccgcgtact gccaaagcgc ttttaacagc gccactttct ctttgtcatc gacatattgt
      721 tgtgccagca tctgcaattc atctgcataa ccctgtaaaa actctggcgt gaagacctgg
      781 cggcggtgct ccagccagcg ttgctgctcg gcataatcca gcgtccccgg gaagttgcgc
      841 gcccgataat tgaacaacag cttttcaatc cgtttatcga caaaagtgat atccagtgcc
      901 ggtaaattac gcggctcggt ttccagcaca attttcattg ctgcacgatc ggcgtcactg
      961 aaaaagccgt tataaagctg tgcatccacg ttatctgaag gcgtaaacgg ttcggcttcc
     1021 gcgaatatcg ccaccacttt ttcgcgcact tgcggatttt cacgcagaat tttcaggtta
     1081 tcgaggcaat gctgacgatt aattcccagt cggtcggcat cttccgggcg tagcgtattc
     1141 gcctgggcca gcaccggaca tttattgata tgcaccagct taaccggaac ggcggcgtta
     1201 tcgccaagat cggtttttgc ggtatataaa cgctcgcgca atgtgtcgct atccagttcc
     1261 agtaatggcg aaatgtctcc tgccaaatcc accataatta cggcattgcg attttcagga
     1321 tgccacgcca gcggtgccac ccagctggta ttgccgcgcc atgctccaaa cattccggaa
     1381 acgtgcacca ggggtttcat ctgcggaaca tcgatcaacg ccatcagttt gtgtttatta
     1441 cgatgggtaa agagataatc aaacaggcgt ggctgacgcg tttttaccag ctttgccatc
     1501 gcaatagtgg cgtacacatc agccatcgca tcgtgggcgt tgctatgttc aataccattc
     1561 gctttggtta aatgctcaag gcgaaagctc ggtagaccgt catcattttc aggccagttt
     1621 attccttccg ggcgcagggc ataacaggca cgcataacat ccagcaaatc ccagcgcgag
     1681 ttatcatgct gccagctcca ggcgtaagga tcgtagaaat tacgataaaa aacgttgcgt
     1741 gtgacttcgt cgtcgaaacg cacattgttg tagcccagaa tacaggtctt cggtacggta
     1801 aaaagcgagt gaatacgggc ggcaaacgcg gcttcgtttt ctcctttcgc ccgtgcttcc
     1861 tgcggggtaa taccggtaat taatacggct cctggctggg gtaaatagtc atcagcgggc
     1921 ttgcagtaaa agacttcggg ttcgccgatg acattgaatt cgctatcggt gcgaatggct
     1981 gcgaactgtg cagggcgatc taacgcgggg tgcgtgccaa aagtttcgta atcgtgaaac
     2041 aaaaaggtag attgttgctt accgtcattc atcattaggt taaatccgtt atttctgctg
     2101 tctgccagag tatcaaatat caccgtgcta atcagcttta gcgcaacaat ttgacagcga
     2161 gtggcaacag atcatgtcag ataaaaatga gagagtagtc acattttctt gcactttatt
     2221 ccagccagtt cataagtatt tccgtaaaaa gaacagctat ttgaaactcc tgagggtttg
     2281 ctgttgaaac gccgtcttat tattgctgct tctttgttcg tttttaactt atcgtctggt
     2341 tttgcggcgg aaaacattcc tttttcacct cagcctccac agattcatgc cgggtcctgg
     2401 gtactgatgg attacaccac cggtcagatc ctcaccgcgg gtaatgagca tcaacagcgc
     2461 aatcccgcca gcctgacaaa gctgatgacg ggttatgtcg tggatcgcgc tatcgatagt
     2521 catcgcatta cgccagacga tattgtcacc gtggggcgcg atgcgtgggc gaaagataat
     2581 ccggtgtttg tcggttcttc actgatgttt ttgaaagagg gcgatcgcgt atcggtacgt
     2641 gatttaagcc gtggtttaat tgtggattcc ggaaatgacg cttgtgttgc tctggctgac
     2701 tatattgccg gtgggcaacg gcagtttgtt gaaatgatga acaactatgc cgagaagctg
     2761 catctcaagg atacgcattt tgaaacagtg catggtctgg atgcacctgg ccagcatagc
     2821 tcggcttatg atttagctgt gctttctcgc gctatcatcc acggcgagcc cgagttttat
     2881 catatgtaca gtgagaaaag tctcacctgg aacggtatca cccagcaaaa ccgtaacggg
     2941 ttgttgtggg ataaaaccat gaatgttgac ggcctgaaaa cgggtcatac ttctggtgcc
     3001 ggttttaacc tcattgcttc ggctgtagat gggcagcgtc gtctcattgc agtggtaatg
     3061 ggggctgaca gtgcaaaagg tcgtgaggaa gaggcaagaa aattactgcg ttgggggcaa
     3121 caaaacttta ctacggtgca aattttgctc cgtgggaaaa aggtcggaac ggaacgcatc
     3181 tggtatggcg ataaagaaaa tatcgacctg ggaacggaac aagagttctg gatggtgcta
     3241 ccgaaagccg aaattccaca tatcaaagcc aaatataccc ttgatggtaa agagctcacc
     3301 gcgccaatta gcgcccatca gcgggtaggg gaaattgaac tttacgaccg tgataaacag
     3361 gtggcgcact ggccgctggt taccctggaa tctgtcgggg aaggcggcat gttttcccgc
     3421 ctgagtgatt atttccacca taaggcctga cctttctttt gcagcagact ggcaggagtg
     3481 cgagtctgct cgcataatca acactcattc cttgtggttt taattttgca actatactgt
     3541 atataaaaac agtattaatg ggggcgtcat gaactacgag attaagcagg aagataaacg
     3601 taccgttgca ggtttccatc tcgttggccc gtgggaacag acggtaaaga aaggttttga
     3661 gcagttgatg atgtgggtag atagtaaaaa tattgtgccg aaggagtggg ttgctatcta
     3721 ttacgacaat ccagatgaaa cacccgccga aaaattacgc tgcgacaccg tcgtgacggt
     3781 gccgggttac tttacgcttc ccgaaaacag tgagggcgtc at
//
LOCUS       DAMZET010000276         3822 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.276, whole genome shotgun sequence.
ACCESSION   DAMZET010000276 DAMZET010000000
VERSION     DAMZET010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.276"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1187
                     /gene="yfcC"
                     /locus_tag="POB34_004633"
     CDS             <1..1187
                     /gene="yfcC"
                     /locus_tag="POB34_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDI5921456.1"
                     /translation="IMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFI
                     LFSLGGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCV
                     VVAQGIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFRE
                     KQADVEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGV
                     VFRLNGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSI
                     ANAISGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLA
                     FQFGDGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMG
                     YH"
     gene            complement(1220..1762)
                     /gene="yfcD"
                     /locus_tag="POB34_004634"
     CDS             complement(1220..1762)
                     /gene="yfcD"
                     /locus_tag="POB34_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HDI5921457.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(1820..2371)
                     /gene="yfcE"
                     /locus_tag="POB34_004635"
     CDS             complement(1820..2371)
                     /gene="yfcE"
                     /locus_tag="POB34_004635"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDI5921458.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(2427..3071)
                     /gene="yfcF"
                     /locus_tag="POB34_004636"
     CDS             complement(2427..3071)
                     /gene="yfcF"
                     /locus_tag="POB34_004636"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HDI5921459.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEVKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            3207..>3822
                     /gene="yfcG"
                     /locus_tag="POB34_004637"
     CDS             3207..>3822
                     /gene="yfcG"
                     /locus_tag="POB34_004637"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HDI5921460.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLK"
BASE COUNT          832 a         1008 c          969 g         1013 t
ORIGIN      
        1 tcatcatgtt tatgctggtg attggcggcg cgtttggcat tgtgatgcgt acaggaacca
       61 ttgataacgg tatcctggcg cttattcgcc atacccgcgg gaatgaaatt ctctttattc
      121 ctgcgctgtt tattctgttt tcacttggcg gcgcggtatt tggtatggga gaagaggccg
      181 tcgcctttgc cattatcatc gcaccgctaa tggtccggct gggctatgac agtattacca
      241 ccgtcctggt gacctatatt gccacgcaaa tcggttttgc cagttcgtgg atgaacccgt
      301 tttgtgtggt cgttgctcag gggattgccg gcgttccggt gctttctggc tccgggttgc
      361 gcatcgtggt gtgggttatc gccactctga ttggcctgat ctttaccatg gtgtacgcct
      421 cacgagtgaa aaagaatcct cttctgtcac gcgtgcatga gtccgaccgc ttctttcgtg
      481 aaaagcaggc tgatgttgaa caacgtccgt ttacctttgg tgactggctg gtattgattg
      541 tcctgaccgc cgtaatggtc tgggtgattt ggggcgtgat cgttaatgcc tggtttattc
      601 cagaaattgc cagccagttc ttcaccatgg gtctggtgat tggcatcatc ggcgttgttt
      661 tccgccttaa cggcatgacg gttaatacca tggcttcatc ctttaccgaa ggggcgcgaa
      721 tgatgatcgc ccctgccctg ctggtgggtt tcgccaaagg gattttgctg ctggtcggta
      781 atggtgaagc aggtgatgcc agcgtgttaa ataccatcct caacagcatt gccaatgcca
      841 ttagcggtct ggacaacgct gtcgcggcct ggtttatgtt gctcttccag gcggtattta
      901 atttctttgt gacatccggt tctggtcagg cggcgttaac catgccgtta ctggcaccgc
      961 ttggcgatct ggtcggtgtt aaccgtcagg ttaccgtgct ggcattccag tttggtgatg
     1021 gcttcagcca catcatttac ccaacctcag cttcgttaat ggcgacgctc ggtgtttgcc
     1081 gggtggactt ccgtaactgg ctgaaggtgg gcgcgacact gcttggactg ctgtttatta
     1141 tgtccagcgt cgtggtgatc ggcgctcagt tgatgggcta ccactaaaaa tgttaagagc
     1201 cgcaattgcg gctctttttt cattctgccg tttcagtctc tacggcttca tttttggcat
     1261 tgcgcttcat ccacaacgcc agcgctttca gcgagtctgg agtgaactca tcgcagcgtg
     1321 cggtgatttc ttccggcgtc agccagcaaa cttcactgac ttcatcttcc tgtagggcga
     1381 agggaccgtg agagacgcag ctgaacaatg cgccccagac acggcaattt ttatcttcga
     1441 aatagaactg cccgtgctca gcaaagggga caccggcaat gcccaactct tcttccgctt
     1501 cgcgacgcgc ggattccagc agttgctcat cggcctggac cacaccgcct gcggtcgcat
     1561 ctaacatgcc gggtaaaaag tcttttgtct cggtacgacg ctggaccaga attttgccca
     1621 tgccatcatg cacgacgatg taagttgcac gatgacgcag acactgtgcc cgcatttgtt
     1681 cccggctggc ttgtgcaatg acttcgttct cttcattgac aatatccacc cattcagtac
     1741 ttgccaaacg acgctgttcc attatcagga aaccttcttt ttctggcgct cttacggcgc
     1801 attttgagtt gtgggtaaat tacggattaa tcgcgacctg cgcaatgata ctttgatcat
     1861 tgagtgcgat tacactaagt acgtcattat ccagcatgcc ataactcgcc ggattaccgc
     1921 ctttcggaat actcaccgag ccggggttga agtggaaaat ctccccccgc tgttctgcca
     1981 ctggcagatg ggtatgaccg tacaccagca catcgttctg gtttaaagca ggtagatttt
     2041 ccgggccaaa aagatgacca tgcgtcaaaa acagacgttg tttttccagt aatacctgtt
     2101 gccacggcgc ggttatcggg aaatgcagca gcatttgatc cacttcgctg tcgcagttgc
     2161 cgcgcacagc gataacctta tgtgccactt cattaagccg ttcagcgact ttggctggcg
     2221 cgtaaccctc cggtaaagca ttacgcgggc cgtgattcaa cacgtcgcca aggatcacca
     2281 gccactgggc accgctttgg gcaaacaact ccagaacacg ttccgtcgcc ggtaacgacc
     2341 catgaatgtc cgatgcaaac atcagtttca tcactcactc ctcgtcgaaa aaacgtaagc
     2401 tatgatactg gattctgtcg ttgctatcag ccagattgct tcgccgaaag tgcaataaaa
     2461 cgctggacag acgctcgctg ccactggaat gtcgcataat ccaccaggcg ttctggcacc
     2521 tcatcgccat gtagtaccag gcggttaatc attagcgcca gatcagtatc agcaatgcac
     2581 cattcaccaa ataaattcgg ctgacccagt gctaacaaat gttctgccat cgcgaacagt
     2641 ttctctgcac tggcttttac ctcggccgtt agtggcgctt ttttcgcccc cgcaaagaca
     2701 acatccgtcg gacgctcttc gcggatgggc atcagatcgc tgcgcagcca ggcctgaatc
     2761 tgtcgtgcac gcgcacgatt ttctaaatca agcggataaa tacgttccca ggtcggtggc
     2821 gcaaatcgat cttccagata ctccgcaatg gcagaagatt cactcaactc aaaatcatcg
     2881 atttgtaata acggcacacg gcgtgtctga ccgtaacctt gccacgtcgg ctgcaaatgt
     2941 tcaccgctgt cgaggtcgat ggtcttgata tgaaacgaca ggcctttttc ctgcaacgcc
     3001 acccaggcgg ataacacata aggggagaaa aagtgggcat ctgaccaaag cgtgatagcg
     3061 ggtttactca ttatgtcctc ggcaataaag ggggctggct atcaaactat agagcctttt
     3121 tgtcactgtc acgtgacgaa tactcacgta agtcgccgcg ctttctatac ttgtggtgtt
     3181 cagatgatta aacaaccgga gctgcaatga tcgatctcta tttcgccccg acacccaatg
     3241 gtcacaaaat tacgctgttt ctcgaagaag cagagcttga ttatcgcttg attaaggtag
     3301 acctggggaa aggcggtcag tttcgtccgg aatttttgcg catttcgccc aacaacaaaa
     3361 ttccggcaat tgttgatcat tctcctgccg atggcggcga accgttaagc ctctttgagt
     3421 ctggtgccat tttgttgtat ctggctgaga aaacaggact ctttttgagt catgaaacgc
     3481 gtgagcgcgc cgccacatta cagtggttat tctggcaggt aggcggactg gggccgatgc
     3541 ttgggcaaaa tcatcatttt aatcacgcag ccccccaaac cattccttac gctattgaac
     3601 gttatcaggt tgaaactcag cgtctttacc atgtactgaa caagcggctg gaaaactcgc
     3661 cctggctggg aggcgagaac tacagcattg cggatattgc ctgctggccg tgggttaatg
     3721 cctggactcg ccagcgaatt gacctcgcaa tgtatccggc agtcaagaac tggcatgagc
     3781 ggatccgttc gcgccctgcc accgggcagg cactgctaaa ag
//
LOCUS       DAMZET010000277         3808 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.277, whole genome shotgun sequence.
ACCESSION   DAMZET010000277 DAMZET010000000
VERSION     DAMZET010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.277"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..184)
                     /locus_tag="POB34_004638"
     CDS             complement(<1..184)
                     /locus_tag="POB34_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="catalyzes the formation of a
                     meso-diaminopimelyl-3-a meso-diaminopimelyl-3 cross-link
                     in peptidoglycan synthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HDI5921461.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTA"
     gene            complement(333..749)
                     /gene="sufE"
                     /locus_tag="POB34_004639"
     CDS             complement(333..749)
                     /gene="sufE"
                     /locus_tag="POB34_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HDI5921462.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(762..1982)
                     /gene="sufS"
                     /locus_tag="POB34_004640"
     CDS             complement(762..1982)
                     /gene="sufS"
                     /locus_tag="POB34_004640"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HDI5921463.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(1979..3250)
                     /gene="sufD"
                     /locus_tag="POB34_004641"
     CDS             complement(1979..3250)
                     /gene="sufD"
                     /locus_tag="POB34_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDI5921464.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(3225..>3808)
                     /gene="sufC"
                     /locus_tag="POB34_004642"
     CDS             complement(3225..>3808)
                     /gene="sufC"
                     /locus_tag="POB34_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDI5921465.1"
                     /translation="TGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFL
                     QTALNAVRSYRSQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDIL
                     QMAVLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDY
                     VHVLYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
BASE COUNT          872 a         1073 c          939 g          924 t
ORIGIN      
        1 ccgcagtatc aaaacgtcgg gcaatagcct gaaggttttt atccccttct tgcaccgtat
       61 acgtttgatt ttgcccaacc agtcggcttc cggttggtgg tagcggataa tcaaccgccc
      121 aggcagcctg gatggcgcta aaagcgccga taagcgtgag tgtaagcaaa gacgcgcgtt
      181 tcattgtaaa cctcctgtat ttgccggaga ctcacgctga aacgtcggat ggcgcttatg
      241 ttcacctgaa accaaaacac tcctgtgcag gtcagtgtaa acattgacca tccggcaatg
      301 tgagccaacc ggatgaaagc tgtcccttta gtttagctaa gtgcagcggc tttggcgcga
      361 attgcgcgaa tcatcgcttc cagaccttgt gaacgagatg gggtgagatg ttgggtgagc
      421 gccatttttt caaaccacgg acgcacatcg aaattgacaa tatcctgcgg cgtcatctga
      481 tcgtagagaa taaatacgac cgcaataagc cctttcacaa tcgccgcatc gctgtcgccc
      541 tgcaattcaa taattccctg cgcattctgg cgcatgacaa tccacacctg actctggcag
      601 ccctgaatgc tattttgtgg gcttcggtct tcgtcgcgta attctggcag acgctggccc
      661 aactcaataa tgtagagata tttctcttcc caattggcgc agcgtaaaaa attacgcagc
      721 aatttttctt tatccggcaa taaagccata gtgcctccct gttatcccag cagacggtga
      781 atacgttgca gtccggtcac cagacgatcc acttcttcat gggtgttata catggccagc
      841 gacgcccgac acatagcagg gacgttgtaa taggccatta atggcatagc gcagtgatgt
      901 ccggtacgca cagcaatgcc gtagttatcg agaaaactgc caacatcata ggcgtggtgt
      961 ttaccaagat taaaagcaat aacgccaagc ctgttctgcg gaccatagag agtgagatcc
     1021 ggtacagatt ccagctgtga tagcgcgtag tgcatcagat tctgttcata ctcggctatg
     1081 ttattaagcc ccagcgccga aacatactcc agcgccgcgc caagaccaat gatgcccccg
     1141 gtattgggtg taccggcttc aaaccgccaa ggtgctttgg tccaggttgt gccttcactc
     1201 aggctgacgg tggcgatcat agaaccgccc ccttcccacg gcggcatctc ctgcaacaag
     1261 gcttctttca cataaagaat gccaattccg gtggggccat acagtttatg cccggagaac
     1321 acgtaaaagt cgcaatccag cgcctgaaca tccaccggat gatgcatcac cgcctgagcg
     1381 ccatccacca gcacttttgc gccatgctgg tgcgcaagcg tgatcatttc cgccagtgga
     1441 ttttctgtgc caagcacgtt ggagacatga gtaattgcca gcaggcgagt tttctcatca
     1501 aacagcgtag gcagcgtctc cagttgcaac gtaccgtcgg gattgagcgg gatcacacgc
     1561 agctctgcgc caacgcgtgc gcaaagcatc tgccagggaa caatgttagc gtggtgctcc
     1621 atctgactga tgatgatgtt atcgcccgcc cgcacattgc tgttgcccca gctattggcg
     1681 accagattga tcccttccgt cgtgccgcgg acgaacacca gctcttccgc cgaacgggca
     1741 ttaataaaca gcgatgcccg cttgcgcacg ttctccattt tctcggtcgc ctgagcgctt
     1801 aaggtatgaa taccgcgatg caccgccgcg tagccatgac gataaaactc ggcctcggcg
     1861 tcaatcacct ggctcggttt ctgtgcactg gcggcgctgt cgagataagc cagcggcaaa
     1921 ccgtttacct cacgcgaaag caccggaaag tcggcccgca ctttgtcgac ggaaaaagtc
     1981 atcttgcacc tcctggcagc cgttgaccga ttcgggccag cacctgctgt ttaagcccct
     2041 catcacgcag tgcttccgtc agttcggcag caaaggcgta aatgatcatc tgctgggcat
     2101 cctgctgatt gatcccgcgc gagcgcagat agaacatctg ttcatcatca atacgcccca
     2161 ccgtcgcgcc gtggctgcat ttcacatcat ctgcatagat ttccagctgc ggttttgtat
     2221 ccacttccgc cagtttgccc atcagcagat tattgttggt catctgacca tccgttttga
     2281 tggcgtgctg cgcgacgttg atcaaaccgt taaataccgc gcggcctttg tcgctgacga
     2341 tagttttgtg caactgtcgg ctgttacaaa aacctttatt gtgttccagc caggtacggg
     2401 tatcacacac ctcgtttttc accggcatcg ccaggctatt gatccgcagc gtgctgtttt
     2461 cgccattgag ttgcgtactg gtgttgtgtc gtaacactgc gccacccagc aggaaactat
     2521 ggctaaatgc ggtggcatcg tcagccagca acaaatcgtt atgggcaaag tggtgactga
     2581 ccgggttttc aaacgccagc ttgatatgct gcaagtgggc gttcgctgcg acattgatag
     2641 tgaaccgtgc gccggtgaag tgacgagcat cattaaggct gacaaaatgt tcgatcaccg
     2701 ttgcttcggc accttccgcc agatccagat gatgtcggta atgggcagtg ttgacctctt
     2761 cacctgccac gccctgggta atatgcatta acagcaatgg ctttgccggc cgttgaccgc
     2821 gcttcacggc gatatgcgtc acgctttgtg ccaggctttc cgtcaaatgc agaaacactt
     2881 ccgcctgaat agcgtcgggt accccctgac ggtcgtcgtt aatgctcact tcatacccgc
     2941 tgccttcagt cgcatcgctc agtgctgaca cgtaacgtcc atcgacaaat accagccgca
     3001 cagcgtctaa cgttaacgct aaggcatcac gctgctgtgg ggatatctcc cccgcaatgc
     3061 tgacaaactg gctattggtc agcccttcca gcggcgtata tttccagttt tcatgtttac
     3121 gtgtcggcag tccggtacgc agcaattgtt gtaaatgctg ctgtgcttgc ggggagcgtt
     3181 ttgtcccttc agcttcaaac aagtgatgcc actgttgcag cgcgttactg ctgttcggta
     3241 agccagccat aaccctgctc ctccagttgt ttgaccaacg tgaaatcgcc ggatttcaca
     3301 attcgtccct gatatagcac atgaacgtaa tcaggcttga tgtagtcgag aatgcgttgg
     3361 tagtgcgtaa caatgatgaa tgagcgcttg ccatcacgca gcgagttcac gccatcggcg
     3421 accactttta atgcgtcaat atccagcccg gagtccgact catcaagaat gcataactcc
     3481 ggttccagca ccgccatttg caaaatatcg ttgcgctttt tctcgccgcc ggaaaaacca
     3541 acgtttaccg aacgggttaa taaatcttcc ggcatcttca ggagagcgat tttctcttcc
     3601 atcaaatcct gaaaatcaaa gcggtcgagc gtttcctggc tgcgatagct gcgcaccgca
     3661 ttaagtgccg tttgcaggaa aaactggtta ctgacgccgg gaatctccac cggatactgg
     3721 aaggccataa agatgccttc gcctgcacga tcttccggcg acagcgcaag caaatctttg
     3781 cctttgaact caaccgtgcc gcccgtca
//
LOCUS       DAMZET010000278         3798 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.278, whole genome shotgun sequence.
ACCESSION   DAMZET010000278 DAMZET010000000
VERSION     DAMZET010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.278"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(362..607)
                     /locus_tag="POB34_004643"
     CDS             complement(362..607)
                     /locus_tag="POB34_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HDI5921466.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            complement(741..2126)
                     /gene="puuP"
                     /locus_tag="POB34_004644"
     CDS             complement(741..2126)
                     /gene="puuP"
                     /locus_tag="POB34_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HDI5921467.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            complement(2116..2280)
                     /locus_tag="POB34_004645"
     CDS             complement(2116..2280)
                     /locus_tag="POB34_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921468.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            complement(2429..>3798)
                     /gene="puuA"
                     /locus_tag="POB34_004646"
     CDS             complement(2429..>3798)
                     /gene="puuA"
                     /locus_tag="POB34_004646"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HDI5921469.1"
                     /translation="RRGSAFTQEVKRYLERYPNTQYVDVLLTDLNGCFRGKRIPVSSL
                     KKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVPVLGSLTPSAADPEFIGQ
                     MLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVELEFYLLDRQRDAEGYLQP
                     PCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINL
                     YHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGE
                     NVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNN
                     RTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGL
                     EQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKNDELLQFERLITETEIEW
                     MLKNA"
BASE COUNT          960 a         1039 c          964 g          835 t
ORIGIN      
        1 cactcacaag tccagcaatc accaaaagag acgataatac ctggcgcata atattaaggg
       61 attttatgta aagaggctat cttactaata tttaatgaca tttgccatta ccgtttgtgt
      121 tcaggggtcg taatgagggc cgtgtggttg gtcgttgaac aggtggcgtc gccatctgtt
      181 caacattcgt acccgtgatt tctctataac tataactcac agaacaactt agcgaggagc
      241 agagcggtac gatcgtgaga aacccattaa agcacgctgc agtgcgtgtc attgttagcc
      301 ggatgcggcg tgaacgcttt atccggacaa cgatactgac cgatcgtctg caattaaata
      361 attactcatt accccattga ttcaaaaact ctgcgatctc atcaatgcgt acgggattaa
      421 tccccgcttc agcagccatt tcatgttggg cttcttcgct gatctcttca ttgttcatca
      481 aacgggtgag cagtaactgg aagtagtggg ccagcggtgt gcgttcagcc tctgcgacag
      541 gctctgcgca ttcggtcgcg tactcatcgg caatatcaaa atattttagc gggatatcgt
      601 ggttcattat ttgcccctgg ggttaatgcg ctaagcataa gggcggatga tagcactcgt
      661 gatccgggtc atacatcttt ttatagttaa tggcgcggcg cgttaccctc aggcaggata
      721 atgcgccgcg catccggcta ttacgtttcg ctcaccggcg ttctgtcacc ttcgtacagc
      781 ggcactttgc gatagcggcg gatcagatac cacaaatatg cgccgcctaa cgaagcccat
      841 accagaccga gtgtcagtga cgttgactct aggttaaccc acagcacacc caccgtcagc
      901 gcgccaacca gcggcatcag caaatagtgg aagtgatcct tccagctttt attcattcct
      961 ttacgccgcc agaaatgatt aaacaccgac agattcacga aggtaaacgc caccagagca
     1021 ccgaagttaa tcagtgctgt cgcggtgact aaatcaaaga acagcgccga cagcgcgaca
     1081 atcccgacca taatgacgtt gagtgccgga gtccgccatt ttgggtgcac atagccaaac
     1141 acgcgctccg gaaacacatt gtcacgcccc atcacataca gcagacgcga cacgctggca
     1201 tgtgaagcca ggccagacgc taatgtgttc acaaacgtgg tgcagaggaa aatcgactga
     1261 aagagcttgc cgccgacgta cagcgcaatt tcaggcagtg cggcgtccgg gtctttaaag
     1321 cggctgatat cgggaaagaa cagctgcata aagaacgacg ccgcgataaa gataacgccg
     1381 ccgtataccg ccgtcaggaa gatggctttc gggatcacgc gtgcggcgtc cggcgtctct
     1441 tccgaaagcg tggtcaccgc atcgaaaccg aggaacgaga aacagacaat cgtcgcaccg
     1501 gtaataatcg ggatcaggtg cgcgttctcg ctgataaacg gctgaagtga ccagacggtg
     1561 ccaacgcctt ctcctttatg cagtccctga accaccagga agataaacac caccatgatg
     1621 gagatttgca ccagtacaaa cagggtattg aagttagcga ccaggttgac gctcttcaga
     1681 ttcgcggcgg ttaaaatggc gacgaaggtt accacccaca cccacggcgg cacttccggg
     1741 aagagggcgg agagatagat tttcgccaac aaaacgttga tcattggcaa aaagagataa
     1801 tccagcagtg atgaccagcc gaccataaat ccgacgtgcg gattaatcga cttttgcgcg
     1861 taggtatagg ccgaaccggc ctccggaaac tggcgaacca gtttgccgta gctgatagcg
     1921 gtaaacagca cgcccgccag cgccagcaaa taggacgctg gaacgtgacc gtcgctaatg
     1981 ccggagacaa taccaaaagt atcaaatacg gtcatcggcg tgagataggc cagacccatc
     2041 attaccacct gccacaattt cagtgatttt cgcagacggg ttttgccggg ttgcgcagca
     2101 atattcagtg gtgaattaat agccataatt gctgcctccc ctgcgtaata aggtttgcgg
     2161 tcgtgtcgac ctgaaatcaa acctgtttag caagggaagc aaagggggcg gagtccagtc
     2221 agtcgcagga ggggcgcata cgcagcctcc ctcgccatgc ggggatttaa tcatcggcat
     2281 catctcattt cctcgtcaca gtcttaatcg ttaacggaag cccgttgccg cccgcgctcc
     2341 ataacgcgac atccgggaaa tgtctggatt accagcaaat gtcaggcctg gctccgctca
     2401 ggccgatgaa acaaccccgc aaggggtatt acgcgttttt caacatccac tcaatttctg
     2461 tttctgtgat gaggcgctca aactgcaaca gctcatcatt tttacaggcg tgatacacat
     2521 ggcaaaaccg ttcgcctaaa tagcggcgca ggtgatcatt ctcgataaac tcacccaggg
     2581 catcgctctg gcgaatcggg aagggtaagc cttcctgttc cagcccgttg ccttcgactt
     2641 cttcctgcaa cggcagctcg ttatcaaggc catgcaaaat accggcaaaa atcgctgcca
     2701 tcaccagata tgggttggca tcggcacccg ccacgcgata ttccacgcgg tgattatgac
     2761 ggtcgccgca cggaatacgc agggcgacgg tgcggttgtt atggccccac gacgcctgcg
     2821 tcggcacata cattcccggc tggaagcggc gatacgagtt cacgtttggt gccagcaacg
     2881 ccatcgacga cggcatcagg tcaatcatcc cggcgagcat ctttttcagc agcggcgaat
     2941 cttcgccttc cgcgtcagaa agcacgttct cgccacggtt attttgcata ctgatatgga
     3001 tatgcattcc gctgcccgcg tgctcttcat acggcttcgc cataaaagtg gcgtgcatct
     3061 tatgcttttc tgccatcaga cgcaccaggc gttttagcgc cagcgcatca tcgcaggctt
     3121 ccagcacgtt atcggtatgg taaaggttga tttcaaactg acccggcgag gcttcggcga
     3181 ccgcaccgtc ggcgggaatt aactgtaact gcgccagttc atcaatatca ttgagcacat
     3241 cggcgaagtg gttgaggtta tcaacggagt aaacctggct ttgtgtattg cggtcatcgg
     3301 tgccgggcgc gcagggcggt tgcagatacc cttcagcgtc gcgctgacga tccagtaaat
     3361 agaactccag ctctaccgct acaaccggga acaatccgcg ctggcgtagc tgctgccaca
     3421 ggcggttaag aacgttccgc ggctcaacgt caaagggagc gccatcttca tcgaccatgg
     3481 tcaggagcat ctgaccgata aactctggat cggcggcaga aggggttaag gaaccgagaa
     3541 caggaacaca agtacgatcc ggctcgccca tttcctgacc cagacccgct tcttctacta
     3601 cgttgcccag aatatccatt gcaaacaccg aggccgggaa ataacaccct ttctccaact
     3661 tcttcaggct tgaaacaggg atacgttttc cacggaagca accattcaaa tcagtaagta
     3721 aaacatcaac atattgcgta ttcgggtagc gctccaggta gcgtttcact tcctgcgtaa
     3781 aggcgctacc ccgcctct
//
LOCUS       DAMZET010000279         3679 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.279, whole genome shotgun sequence.
ACCESSION   DAMZET010000279 DAMZET010000000
VERSION     DAMZET010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.279"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..221
                     /gene="nemR"
                     /locus_tag="POB34_004647"
     CDS             <1..221
                     /gene="nemR"
                     /locus_tag="POB34_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HDI5921470.1"
                     /translation="KGARGVIALLSQALENGRENHCLTFCGEPLQQAQVLYALWLGAN
                     LQAKISRSFEPLENALAHVKNIIATPAV"
     gene            258..1355
                     /gene="nemA"
                     /locus_tag="POB34_004648"
     CDS             258..1355
                     /gene="nemA"
                     /locus_tag="POB34_004648"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HDI5921471.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            1436..1843
                     /gene="gloA"
                     /locus_tag="POB34_004649"
     CDS             1436..1843
                     /gene="gloA"
                     /locus_tag="POB34_004649"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDI5921472.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            1946..2593
                     /gene="rnt"
                     /locus_tag="POB34_004650"
     CDS             1946..2593
                     /gene="rnt"
                     /locus_tag="POB34_004650"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HDI5921473.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPTAEEV"
     gene            2686..>3679
                     /locus_tag="POB34_004651"
     CDS             2686..>3679
                     /locus_tag="POB34_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDI5921474.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAA"
BASE COUNT          865 a         1003 c          989 g          822 t
ORIGIN      
        1 ataaaggtgc tcgcggcgtg atcgccctgc tctctcaggc gctggaaaat ggccgtgaga
       61 accattgttt aaccttttgt ggcgaaccgc tgcaacaggc acaagtgctt tacgcactgt
      121 ggcttggcgc gaatctgcag gccaaaattt cgcgcagttt cgagccactg gaaaacgcgc
      181 tggcccatgt aaaaaacatt attgcgacgc ctgccgttta gcaggcattt tttatcacca
      241 gacgaccggg agcctttatg tcatctgaaa aactgtattc cccactgaaa gtgggcgcga
      301 tcacggcggc aaaccgtatt tttatggcac cgctgacgcg tctgcgcagt attgaaccgg
      361 gtgacattcc taccccgttg atggcggaat actatcgcca acgtgccagt gccggtttga
      421 ttattagtga agccacgcaa atttctgccc aggcaaaagg atatgcaggt gcgcctggca
      481 tccatagtcc ggagcaaatt gccgcatgga aaaaaatcac cgctggcgtt catgctgaaa
      541 atggtcatat ggccgtgcag ctgtggcaca ccggacgcat ttctcacgcc agcctgcaac
      601 ctggcggtca ggcaccggta gcgccttcag cacttagcgc gggaacacgt acttctctgc
      661 gcgatgaaaa tggtcaggcg atccgtgttg aaacatccat gccgcgtgcg cttgaactgg
      721 aagagattcc aggtatcgtc aatgatttcc gtcaggccat tgctaacgcg cgtgaagccg
      781 gttttgatct ggtagagctc cactctgctc acggttattt gctgcatcag ttcctttctc
      841 cttcttcaaa ccatcgtacc gatcagtacg gcggcagcgt ggaaaatcgc gcacgtctgg
      901 tactggaagt ggtcgatgcc gggattgaag aatggggtgc cgatcgcatt ggcattcgca
      961 tttcgccaat cggtactttc cagaacacgg ataacggccc gaatgaagaa gccgatgcac
     1021 tgtatctgat tgaacaactg ggtaaacgcg gcattgctta tctgcatatg tcagaaccag
     1081 attgggcggg gggtgaaccg tatactgatg cgttccgcga aaaagtacgc gcccgtttcc
     1141 acggtccgat tatcggcgca ggtgcataca cagtagaaaa agctgaaacg ctgatcggca
     1201 aagggttaat tgatgcggtg gcatttggtc gtgactggat tgcgaacccg gatctggtcg
     1261 cccgcttgca gcgcaaagct gagcttaacc cacagcgtgc cgaaagtttc tacggtggcg
     1321 gcgcggaagg ctataccgat tacccgacgt tgtaatccaa cattgcgagc ggcgtaaagc
     1381 cgccgctata ctaaaacaac attttgaatc tgttagccat tttgaggata aaaagatgcg
     1441 tcttcttcat accatgctgc gcgttggcga tttgcaacgc tccatcgatt tttataccaa
     1501 agtgctgggc atgaaactgc tgcgtaccag cgaaaacccg gaatacaaat actcactggc
     1561 gtttgttggc tacggcccgg aaaccgaaga agcggtgatt gaactgacct acaactgggg
     1621 cgtggataaa tacgaactcg gcactgctta tggtcacatc gcgcttagcg tagataacgc
     1681 cgctgaagcg tgcgaaaaaa tccgtcaaaa cgggggtaac gtgacccgtg aagcgggtcc
     1741 ggtaaaaggc ggtactacgg ttatcgcgtt tgtggaagat ccggacggtt acaaaattga
     1801 gttaatcgaa gaaaaagacg ccggtcgcgg tctgggcaac taatctcctg ccgggcgtga
     1861 actcatcgcg cccgcatctt tactgcatcg acaagtaata tttgtcataa tgcgcgctgc
     1921 aatttatccg tattaagaga atcagatgtc cgataacgct caacttaccg gtctgtgcga
     1981 ccgttttcgt ggtttttatc ctgttgtgat cgatgttgaa acagccggat ttaacgccaa
     2041 aaccgatgcg ctgcttgaga ttgccgccat caccctgaaa atggatgaac aaggctggct
     2101 gatgccggac accacattac atttccacgt cgaaccattt gtcggcgcaa atttgcaacc
     2161 agaagccctc gccttcaacg gcattgaccc gaacgatccc gatcgcggcg cggtcagcga
     2221 atacgaggcg ctgcacgaaa tttttaaagt tgtacgtaaa ggtattaaag cgagcggctg
     2281 taaccgcgcc attatggtgg cgcacaacgc caattttgat cacagcttta tgatggccgc
     2341 cgcagaacgc gcctcgctga aacgtaaccc gttccaccct ttcgccactt ttgacactgc
     2401 tgcactggcc gggctggcac tcggacaaac cgtattgtca aaggcttgcc agaccgctgg
     2461 catggacttc gacagcaccc aggcgcactc cgcgctgtac gacaccgaac gcactgctgt
     2521 gctgttttgt gaaatcgtca accgctggaa acgtctggga ggctggccgc tacccaccgc
     2581 cgaagaggtg taatcgagtc gatgcctgat gacatataat gattcaggca tctatagtga
     2641 ggctattcca cgcatcctgc atgatattca cggggaatag cgttaatggc agataatcca
     2701 gacccttcat cgctcctgcc ggacgtgttt tcaccggcga cccgcgactg gtttcttcgc
     2761 gcctttaaac agccgaccgc tgtccagtcg caaacctggc atgtggcggc gcgaggcgaa
     2821 catgcgctgg tgattgcacc gactggctcc gggaaaacgc tggcagcatt tctctacgcc
     2881 ctcgatcggc tcttccgcga aggcggcgaa gatacccgcg aggcgcataa gcgtaaaacc
     2941 tcacgcatcc tctatatttc accgataaaa gccctgggca ccgacgttca gcgcaacttg
     3001 cagatcccgt tgaagggtat tgccgatgaa cggcggcggc gcggcgaaac ggaagtcaat
     3061 cttcgcgtag ggatccgtac tggcgatacg cctgcacagg aacgcagcaa actcacccgt
     3121 aatccgccgg atattctgat caccacaccc gaatcactct atctgatgct gacctcccgc
     3181 gcgcgcgaaa cgctacgcgg cgtcgaaacg gtaattattg atgaagtcca cgcggtagcg
     3241 ggcagtaaac gtggtgcgca tctggcgtta agtctggagc ggctcgatgc gctgctccac
     3301 acctcagcac agcgaattgg cctttctgcc actgtgcgct cagccagcga tgtggcagca
     3361 tttcttggtg gcgatcgccc ggttacggta gtcaacccgc ccgcaatgcg tcatccgcag
     3421 atacggattg tcgtaccggt cgccaatatg gatgatgtct catcggtcgc cagcggcact
     3481 ggcgaagaca gccatgccgg ccgggaaggc tccatctggc catatattga aacgggcatc
     3541 cttgatgaag tgttgcgcca tcgctcgacc attgtcttta ctaattcgcg ggggctggcg
     3601 gaaaaactga cggcacgatt aaatgagctt tacgccgcac gcttacagcg ttccccgtct
     3661 atcgccgttg atgcggccc
//
LOCUS       DAMZET010000280         3677 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.280, whole genome shotgun sequence.
ACCESSION   DAMZET010000280 DAMZET010000000
VERSION     DAMZET010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.280"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..146)
                     /locus_tag="POB34_004652"
     CDS             complement(<1..146)
                     /locus_tag="POB34_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921475.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERIS"
     gene            442..717
                     /gene="ypdI"
                     /locus_tag="POB34_004653"
     CDS             442..717
                     /gene="ypdI"
                     /locus_tag="POB34_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HDI5921476.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(794..1036)
                     /locus_tag="POB34_004654"
     CDS             complement(794..1036)
                     /locus_tag="POB34_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HDI5921477.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            1389..2309
                     /gene="lpxP"
                     /locus_tag="POB34_004655"
     CDS             1389..2309
                     /gene="lpxP"
                     /locus_tag="POB34_004655"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HDI5921478.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2665..2736
                     /gene="ypdK"
                     /locus_tag="POB34_004656"
     CDS             2665..2736
                     /gene="ypdK"
                     /locus_tag="POB34_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HDI5921479.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2801..>3677)
                     /locus_tag="POB34_004657"
     CDS             complement(2801..>3677)
                     /locus_tag="POB34_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDI5921480.1"
                     /translation="GDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNELERAI
                     RESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVYDGWKA
                     PSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYGTFTPL
                     QVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKIPEPYA
                     AMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKAMFRAD
                     GLLPASSKHIHENAE"
BASE COUNT          976 a          903 c          829 g          969 t
ORIGIN      
        1 gagatacgct ctgctgctgc cgttttctgg gtttgctgaa cttgctgagt ttgctgttgt
       61 gccgctaccg gtgcattatc agcagcccat acagttgaag atgccagaat tgctgtgacc
      121 atcgtagcca taattaaacg tttcatactt acctcattct ttaacagttt tagaaaacgc
      181 cgccatcata ttgggtgagc atgaaatgag aaatcagaga gcaacggtca ttttgaaagg
      241 gttatcttac agttgtagtt attatcgcaa gaagtatttt agtcttacaa ttgtaggaat
      301 ctgcttcctg tcgcagagaa ctattcttat gtaccacaaa gagatgtcat ttattatatc
      361 gatgtaaata ttgattaatt atatgtaaat acagccataa catcgtaggt tctcagtgaa
      421 ccattttcag gataactttc tatgaaagta aacttaatac ttttcagctt atttttattg
      481 gtctctatta tggcatgcaa tgtttttgca ttttccattt ctggtggtgt aagtgagagg
      541 agctataaag agactgaaaa aacatcagcg atgacgacca cacactctac aaaacttcag
      601 ccatcacagg cgattttgtt taagatgaga gaagatgcgc caccattaaa cctcacagaa
      661 gaaatcacgc ctacttatcc gacaaaggcg aattatctta ttcatcctgt gcgatagcgt
      721 ctgtcacatg gcaggtgaac aatcatgtct gatgctgatg tgaacacctg cccaaatgag
      781 ccatcctgac ccactatcgc ctgccatacc ccaccataaa gcgaatcatc aagccacaag
      841 ccggggcaag aaacataaat atccccgtca ctagttcatt accgccagtt aaccacatgg
      901 tgaagtaata aatgagtgca gcgaggatca ccatgccgaa gaaagaagag accgccgata
      961 ccacgttcag tacctgaccg atgtagccgc ttacgcagac aatacacagg gcgagaaaca
     1021 tccataaata aatcatagct caacctcctg ccgcgctaaa aaacctgtaa cttcagtcac
     1081 tttaatagta agacaatcct taacctctgg caatttttgc taagcatcaa tgaaaacagc
     1141 atgttaaatg caagactgtt gtgtacggaa aaatatttac tttgcacgat taataatcag
     1201 tagctgaaag cagtcagcga gtgataaagt acagataccg cattcatgct cctcgtggtt
     1261 atgtcctgac tagtctttac actctttaca ggaaccattg tcgtacatga tggcccaacc
     1321 aattgagtgt accgctgtcg acatggaatc ttctgcgata caacaattcg tatctacaga
     1381 aggtaactat gtttccacaa tgcaaatttt cccgcgagtt tctacatcct cgctactggc
     1441 tcacatggtt tgggcttggt gtactctggc tttgggtaca gcttccttat cctgttctct
     1501 gctttctcgg cacgcgtatt ggcgcaatgg cgcgaccatt cctgaaacgt cgtgaatcta
     1561 tcgcccgtaa aaacctggaa ctttgtttcc cgcagcattc tgcggaagaa cgcgagaaga
     1621 tgattgccga aaactttcgt tcactcggca tggcgctggt agaaaccggc atggcatggt
     1681 tctggcccga cagtcgcgta cgtaaatggt ttgatgttga agggttggat aaccttaaac
     1741 gcgcacaaat gcaaaatcgc ggcgtaatgg ttgtcggcgt ccattttatg tcgctggaac
     1801 tgggcggccg cgtgatggga ctgtgccaac caatgatggc tacctatcgt ccacataata
     1861 atcagctgat ggaatgggtg cagacccgtg ggcgcatgcg ctctaacaaa gcgatgatcg
     1921 gcagaaataa tctgcgcggc attgtcggtg cactgaagaa aggtgaagcg gtatggtttg
     1981 ctcccgatca ggattatggt cgtaaaggca gctccttcgc gccgttcttt gcggtggaaa
     2041 atgtcgccac aaccaatggc acctatgttc tctcccgtct ctctggcgca gccatgttga
     2101 ccgtaacgat ggtaagaaaa gcggattaca gcggatatcg tttgttcatc accccagaga
     2161 tggaaggcta cccgacagat gaaaatcaag ccgctgccta tatgaacaag attatcgaga
     2221 aagagatcat gcgcgcaccg gagcagtacc tctggatcca ccgtcgcttt aaaacgcgcc
     2281 cggtgggaga atcgtcgttg tacatttaac aaactgctct tactctttcc ctcctcaaat
     2341 cggccataat aacctcatgg tttttatggc ttttttctta aggttttttc cttcgctgaa
     2401 tatctaatta ttaccgtcag aaattaaact gtcgccgata tgagacactt gtaagttact
     2461 aaaagtaaat aagaaaatgt ctcttgtctc gtttcagttt taggcgtaca ttagcgccgt
     2521 ctcgagcagt ttagctcaga attctggggt gatgcccacg gtaaaataca gggcttttaa
     2581 ggtcgctttg cccggtgtca actcactgta tcaggtgtaa tgaagtcatt caggcgtaac
     2641 agtaattacg cggagagatg taaagtgaaa tatttcttta tgggcatttc atttatggtc
     2701 atcgtttggg ccggtacttt tgccctgatg atctaaaagc aaactgtgca atgaaaaaca
     2761 ggagccatca ggctcctgtt tgcatttctg acgacggtgt ttattccgcg ttttcgtgaa
     2821 tatgtttgct gctggcgggt agtaaaccgt cggcacggaa catcgcttta atcccacgaa
     2881 tcgcctgacg aatacggtcg cggttttcaa tcagtgcaaa gcgaacatgc gtatcaccat
     2941 agtcgccaaa gccaatcccc ggcgagacac aaaccttcgc ttcgttaagg agcttcttgg
     3001 caaattccag cgatcccatg gccgcatatg gttccgggat tttcgcccag acatacatcg
     3061 aagccttcgg catttcaacc atccagcccg cttcatgcag ccctttaacc aatacatcac
     3121 ggcggcgttt gtactgttcg gcaatgtcgc gcacgcactg ttgatcgccc tccagcgccg
     3181 caatcgccgc cacctgcaac ggcgtaaagg tgccgtaatc gtgatagctt ttaatacgag
     3241 ccagggcgct gaccagcgtt ttgttgccaa ccataaagcc gatacgccag cccgccatgt
     3301 tgtagctttt cgacagcgta aagaactcga ctgccacatc gcgtgcaccc ggtacctgca
     3361 tgattgacgg cgctttccag ccatcgtaga cgatatcggc ataggccagg tcgtggacca
     3421 ccagcacatc gtagcgtttc gccagcgcca caaccttttc gaagaactcc agctccacgc
     3481 attgcgcggt tggattcgac gggaagccga ggatcatcat cttcggtttc ggataacttt
     3541 cacgaatggc gcgctccagt tcgttgaaga aatcgacacc ttccaccagc ggcactgagc
     3601 gtacctgcgc cccggcaatc accgcgccat aaatatggat cgggtaactt ggattcggca
     3661 ccagcaccgt gtcacca
//
LOCUS       DAMZET010000281         3668 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.281, whole genome shotgun sequence.
ACCESSION   DAMZET010000281 DAMZET010000000
VERSION     DAMZET010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.281"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..93)
                     /locus_tag="POB34_004658"
     CDS             complement(<1..93)
                     /locus_tag="POB34_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HDI5921481.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLP"
     gene            complement(101..487)
                     /gene="rutC"
                     /locus_tag="POB34_004659"
     CDS             complement(101..487)
                     /gene="rutC"
                     /locus_tag="POB34_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HDI5921482.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRTVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            complement(499..1191)
                     /gene="rutB"
                     /locus_tag="POB34_004660"
     CDS             complement(499..1191)
                     /gene="rutB"
                     /locus_tag="POB34_004660"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HDI5921483.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFVQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(1191..2282)
                     /gene="rutA"
                     /locus_tag="POB34_004661"
     CDS             complement(1191..2282)
                     /gene="rutA"
                     /locus_tag="POB34_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HDI5921484.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGSGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            2570..3208
                     /gene="rutR"
                     /locus_tag="POB34_004662"
     CDS             2570..3208
                     /gene="rutR"
                     /locus_tag="POB34_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HDI5921485.1"
                     /translation="MTQGAVKTTGKRSRTVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            complement(3248..>3668)
                     /locus_tag="POB34_004663"
     CDS             complement(3248..>3668)
                     /locus_tag="POB34_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921486.1"
                     /translation="RNTWTLLPRERVLCIADDEQDALTQLAAVLAVGSQVLWPDDALH
                     RQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDSDQLRALCEAVAARDGAIVSVQ
                     GFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG"
BASE COUNT          866 a          970 c         1002 g          830 t
ORIGIN      
        1 cggtaaccag taactgccgc taccgccaag acccgaaatc aacaccacta cgggtgcatc
       61 agcataaggg ggaggtgaga gtgatagttt catcgcggcc tcacttggcg atatgcgcaa
      121 ttgtggcgat ttccaccagc gcgtcaggtt ttaccagtcc gcactgaatg cagaatcgcg
      181 ccggtttatc acccggaaaa aactcggcgt agatttcgtt aatcgcggcg taatttttcc
      241 agtcggtaat aaagatgctg ttgaaggtca catccgccat cgtgccaccc gccgtctcga
      301 tcaccgtgcg gatagtttcc agaacgtggc gggtttgcgc ctttgggtca tcggcaaaca
      361 gcacgttatt atgttgatca aaagccagcg taccggagac atacaccacg ccatcagcca
      421 gcgtgccggg aacgaagggg gccagcggtg cgctgctgcc agcgggaata attacggatt
      481 ttggcatcgt taaactcctt aagcgatacg agcaaaggac gtgggagaaa gcgcgtcgca
      541 gaacgtttcg acgtcgctga cccagccaaa aaaggtttcg atattgaaca acgcggcttt
      601 ctgtacaaat tccggccccg cctggtgtgt tgcgtcttcc agcaccacgc cgaaatactc
      661 cagaaaaaag ccatcgcgta gcgtcgattc gacgcagacg ttggtagcga taccggtgaa
      721 aaccagatgg cgtattccac ggctgcgcaa aatgctgtcc agcggcgtat tgaagaaacc
      781 gctgtagcgc ggcttcggca gcacaatatc gccaggctgc ggcaccagtt catccaccag
      841 ttgataatcc caggagcctt tcgccagcaa tttcccctgc agctgcggct gcttacgcat
      901 ggttttcagg gcgttcgatt tatgaaaatt cggtgagccg ggtccgcctg cttcgacata
      961 ctgttcatcc cagccatttt gaaaccagat gatcagcatc cctgccgctc gcgctgcggt
     1021 cacggcggtt tgaatgttgg caatgaccgg gcgagtggtt gagacatcaa acccggcgag
     1081 atctaagtag cctcctggcg tggcataagc gttttgcata tccaccacga tcagcgcact
     1141 ttgctgcgga tcgaaggtaa tggcttccgg tcgagcggtt aaggtcgtca tcatgccacc
     1201 tcctgagtca gcgcagggag atgggcgcgg cactgcatca gtggttgaat gcgctcgccg
     1261 aaggtttcga ttcccgacag aaaatcatcg aaggtcaaca gcacgccttc ggcaccaggc
     1321 acgcttgcga cttcatctaa catgcgcgcg acactggcgt aagaaccgac taacgtcccc
     1381 atattgatgt ttaccgccga agtgggatcg gccatctgac ggacgttggt gtcagtaccg
     1441 gagcgggtat ctttctgact ttgttcggtt agccagctta acgcctcttc atccgcgccc
     1501 gctttgtagt gttcccattt ggcgcgagcg gcatcgtcgg tttcatcggc aatcaccata
     1561 aacaatacat aagagccaac gtcgcgcccg gtttgctctg cggcctgttt catccgcgca
     1621 gcggtcgggg cgaaagccgt gggtgtattt acgcctttgc cgaaacagaa gttgaaatcg
     1681 gcatactggg cggagaacgc catgccagcg tcgctttgcc cggcgcagat cactttcatg
     1741 ggtacactcg gttgcggact gacgcgacaa tcattcatgg tgaaaaaatc gcctttaaaa
     1801 tcgcttttcc ccgagcccca caggtcgcgc agcacctgaa catattcggt gagatagtcg
     1861 taacgacggg agaaatagtc atcgccaggc cagataccca tctgctcata ctcgggcttt
     1921 tgccagccag tcacgaggtt gacgccaaaa cgcccgccag agatggagtc aatggttgcg
     1981 gccatacggg cgacgattgc cggaggtaac gttaaggtgg cagctgtcgc gtaaatctga
     2041 atgcgtgagg tgacggctgc cagccccgcc atcaaggtga acgactcaag gttgtgatcc
     2101 cagaactcag ttttgccgcc aaagccacgc agtttgatca tcgacagggc gaaatcgaaa
     2161 tggtagtgct ccgctttttg cacaatggct ttattcagtt caaaggtcgg catgtactgc
     2221 ggcgcgtggg tcgaaatgag ccagccgttg ttgccaatag gtacgaatac gccaattttc
     2281 atcatcaacc tctcttcgtc tcgtaaagtg aaagtcagac ggggcactgc gtcctgcata
     2341 tccttttcag ccgcgtaatg gcttgtttgc aaagtggatg ccagttttta aaaagtgaat
     2401 gttattaatg tgttaatgct aagttatcta aaatatctgg taaaaagtgg actaaacggt
     2461 caaaacagtt gcacataaaa catgcatccg tgcgcgatga gagtgcagaa ggtcgaggcc
     2521 ggacgggggt tttgctatcc tgttgccaat ctacaagagg ggagagcgca tgacgcaagg
     2581 cgcagtcaaa acaacgggta aacgttcgcg tacagtaagc gcgaagaaaa aagcgattct
     2641 tagcgcagcg ctggacactt tttcacaatt cggttttcac ggcacaaggc tggagcagat
     2701 cgcagagttg gcgggtgttt caaaaaccaa tctgctgtat tactttccgt caaaagaggc
     2761 gctgtatatt gccgtgctgc ggcagattct cgatatctgg ctggcaccgt taaaagcgtt
     2821 tcgtgaagat ttcgccccgc tggcggcgat caaagagtac atccgtctga agctggaagt
     2881 ctcacgcgat tatccgcagg cttcgcgcct gttctgtatg gagatgctgg caggcgcgcc
     2941 gttgttaatg gatgaactga cgggcgattt gaaggcatta attgatgaga aatcggcgct
     3001 gattgccggt tgggtcaaaa gcggcaaact cgcgccgatt gatccgcagc atttgatttt
     3061 tatgatttgg gcttccactc aacattacgc cgatttcgcc cctcaggtgg aggcggtgac
     3121 aggcgcgacg ttgcgcgatg aggtattttt caatcaaacg gttgaaaacg tgcagcggat
     3181 tattattgag gggattcgac cacgttaaag atgccggagg aggttgtaac atcctccggc
     3241 tacctgttta acctatagtc attaagctgg cgttaccgcc agcggcagcg gtattcacac
     3301 tcagcgaacg ctcgatatac agccgttcca gaaggatatt gctttcgcca cgggcaaaac
     3361 cctgcaccga aacaattgcg ccatcccgcg cggcaacggc ttcacacaat gcgcgaagct
     3421 gatccgaatc accgtggaag atcaccgcat caaacggttg agcggttata ttttccgctt
     3481 tcgccagttg aatacgttcg ctgactgccg atggcaatgc cttcactagc tgacgatgca
     3541 gcgcgtcatc cggccacagt acctggctgc ccaccgccag cacggcggcg agctgagtca
     3601 gcgcatcctg ctcatcatcg gcaatacaca acacgcgctc acgcggcagc agcgtccagg
     3661 tgttgcgt
//
LOCUS       DAMZET010000282         3649 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.282, whole genome shotgun sequence.
ACCESSION   DAMZET010000282 DAMZET010000000
VERSION     DAMZET010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.282"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..108)
                     /locus_tag="POB34_004664"
     CDS             complement(<1..108)
                     /locus_tag="POB34_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310737.1"
                     /note="in Escherichia coli this inner membrane protein was
                     found to anchor the periplasmic catalytic oxidoreductase
                     YedY; sulfite oxidase activity not demonstrated; contains
                     heme; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoxide reductase heme-binding subunit YedZ"
                     /protein_id="HDI5921487.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLG"
     gene            complement(109..1113)
                     /gene="msrP"
                     /locus_tag="POB34_004665"
     CDS             complement(109..1113)
                     /gene="msrP"
                     /locus_tag="POB34_004665"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HDI5921488.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(1222..1635)
                     /gene="hiuH"
                     /locus_tag="POB34_004666"
     CDS             complement(1222..1635)
                     /gene="hiuH"
                     /locus_tag="POB34_004666"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HDI5921489.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            1768..2439
                     /gene="hprR"
                     /locus_tag="POB34_004667"
     CDS             1768..2439
                     /gene="hprR"
                     /locus_tag="POB34_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HDI5921490.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            2439..>3649
                     /gene="hprS"
                     /locus_tag="POB34_004668"
     CDS             2439..>3649
                     /gene="hprS"
                     /locus_tag="POB34_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HDI5921491.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVIVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRF"
BASE COUNT         1010 a          769 c          852 g         1018 t
ORIGIN      
        1 acccagtcca ccgtgattga tcgcccagac cagccagaga aacggcaaca atccggcaag
       61 atgcaggcta actttcagcc atgtcacctg ttttgctgtc agacgcattc agaaattctc
      121 ccgcaaatcc aggccacgat acagcgatgc cacctggtcg gcgtaaccat taaacagtag
      181 cgttggctgg cgctgaacat cgaggatgcc gcctgaacca ataaatcgtt cagtagcctg
      241 tgaccagcgc gggtgatcaa catgcggatt aacgttggcg taaaaaccgt attcgtcagg
      301 cgctgccaga ttccaggtgg ttggcggacg ctcgcgggtc agcttaatac tgactatcga
      361 tttaatccct ttaaagccat atttccacgg cacaatcagt cgtaccggcg cgccattttg
      421 tggcggtaac gccttgccat aaacacccac ggtcatcagt gtgagcggat gcattgcttc
      481 gtcgagacgc aatccttcga cataaggata tttcagcccg ccgccgataa agcggtcctg
      541 ctggcctggc atttgttccg gtgcataaat tgtttcgaaa gcgacatact tcgcattgct
      601 ggtgggttcg gcaagcgcca gcaatttgtg cagcggaaaa ccaatccacg gcaccaccat
      661 cgaccatgcc tccacgcagc gcatacgata aatacgctct tccagcggga agcgacgggt
      721 taaatcatcg tgatcgaggg tcaatggttt tgccacttcg ccgctgattt tcagtgtcca
      781 tggatcggtt ttcaggctac cagcattagc ggcgggatcg gctttatcca gcccgaattc
      841 atagaagttg ttataaccgg agactttatc tactggcgtc agtggcaggt tattttgcca
      901 ggcggcaggc ttgctgaact ccagcggttt tccggcgggg gcgggcgggc gatcgttccc
      961 tttaaaccag ctaagcagat cggcatgcgc agcgtgaggc aaagaaagtg cagctgcgct
     1021 gatgcccagt gcttttaaca cctgtcgacg cttcataaag aataccgact cggccgtaac
     1081 atctgattct tttaaaaatt gattcttttt cataagccat cacacttttt tgtcgttagc
     1141 cagcaagagc cgtttactgt taatctgtca gggagtgtcc tccctaacag tagcagagat
     1201 ctgcttttac atatttatgc attaactgcc acgataggtt gaatacccat attggctaag
     1261 taataaaggc acatgataat gctcgttcac tttattaata tgaaattcaa cggggatctc
     1321 agggaagaaa ctttcaagat tttgtttctt gaaatagtcc ccggttttaa atacgacacg
     1381 gtaatcgccc gtagttgcag tttgctcggg ccacagtgcc ttaattcgtc catccttatc
     1441 tgttttggcg gtattaagtt gtaaccagcc gttgtccgct ttcttttcaa gagtgactgt
     1501 cacgtcggca gcgggttttc cggtttgctg gttcaaaatg tgcacgctaa gaatgttttg
     1561 ttgtgcggca taaaccaaag aaggtaatga aaatgctgcc gttactacgg agagtactaa
     1621 ataacgcttt aacatgttta tatccttgtc atgtgaatga gttttgagga tagcgaaaaa
     1681 gcggccaggg taaccttaca gcagcattac aattttgtaa tgaaccaggc tgtttctata
     1741 acatatgatt tatggcatat tattttcatg aagattttac ttattgaaga taatcaaagg
     1801 acccaggagt gggtaacgca ggggctttcc gaagcgggtt atgtcattga tgccgtttct
     1861 gatggcagag atgggcttta tcttgcgctg aaggatgatt atgcattgat cattctggat
     1921 attatgcttc cgggtatgga tggctggcag atcttacaaa cgttaagaac agcaaagcaa
     1981 acccctgtta tttgccttac tgcaagggat tctgtcgatg acagagtcag agggctggac
     2041 agtggagcaa atgattatct ggtaaaacct ttttcatttt ctgagttgct ggcaagggtt
     2101 cgggcacaat taaggcaaca tcacgctttg aattcaacat tagaaatcag tggcttaaga
     2161 atggactctg taagtcaaag tgtgagcagg gacaatatca gtattacact gacgcgcaag
     2221 gagtttcagt tactttggct actggcctcc agagctggcg aaattatacc cagaacggtt
     2281 attgcgagtg aaatttgggg aatcaacttt gatagtgata ccaatacggt ggatgtcgcc
     2341 attcgcaggc tccgcgcaaa agttgatgat ccttttcctg aaaagctaat cgccacaatc
     2401 cgggggatgg gctattcatt cgtagcggta aaaaaataat gaaaagatta tctataaccg
     2461 tccgtttaac cttgcttttt atattgctgc tgtctgttgc tggcgctgga attgtctgga
     2521 ctctctataa tggcctggca agtgagttga aatggcgcga tgatacaaca ctcattaacc
     2581 ggacagcaca gatcaagcag ttgttaattg atggggtaaa tccagatacg ttacctgtat
     2641 actttaaccg gatgatggat gttagtcagg atatcttgat cattcatggt gatagcatca
     2701 ataaaattgt taaccggaca aatgtcagtg atgacatgtt aaataacata cctgctagtg
     2761 agacaatcag cgcagctggc atttacagaa gcattattaa tgatactgag attgatgctt
     2821 tacgaattaa tattgatgaa gtttcgccat cattaacggt tattgtggct aaattggctt
     2881 cagccagaca taacatgctt gaacagtata aaattaatag cattataatt tgcattgtcg
     2941 ccattgtact ttgctcagta ttaagtccgc tgttaatcag aacgggatta cgagagatca
     3001 aaaagttgag tggtgtaacg gaagcgctga attataacga tagccgggag cctgttgagg
     3061 ttagcgcatt accgagagaa ctaaaacctc ttgggcaggc gttgaataaa atgcatcaag
     3121 ccttagtcaa agattttgaa cgcctaagtc aatttgctga cgatctcgct catgaactta
     3181 gaacgcccat taatgcatta ctgggtcaga atcaggttac gctcagtcaa accagaagta
     3241 tcgctgaata tcaaaaaaca attgccggta acattgaaga gctggaaaat atttcgcggt
     3301 taacagagaa catactgttt cttgcccggg cagataaaaa caatgttttg gtgaaactgg
     3361 actcgctttc tctcaataag gaagtcgaaa atttgttgga ttatcttgaa tacctttcag
     3421 acgagaaaga gatttgcttt aaggtcgagt gcaatcagca aatctttgcg gataaaattt
     3481 tactgcaacg aatgttatcg aatcttattg ttaatgccat tagatattca ccagaaaaat
     3541 cacgtattca tataaccagt tttcttgata ccaacggcta tcttaatatt gatgtcgcca
     3601 gtcctggaac gaaaattcat gagcctgaaa aactcttccg tagattttg
//
LOCUS       DAMZET010000283         3635 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.283, whole genome shotgun sequence.
ACCESSION   DAMZET010000283 DAMZET010000000
VERSION     DAMZET010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.283"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..507
                     /locus_tag="POB34_004669"
     CDS             <1..507
                     /locus_tag="POB34_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5921492.1"
                     /translation="SLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHA
                     GLLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPR
                     ERSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLR
                     ITQPRSRA"
     gene            complement(553..1434)
                     /gene="htpX"
                     /locus_tag="POB34_004670"
     CDS             complement(553..1434)
                     /gene="htpX"
                     /locus_tag="POB34_004670"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HDI5921493.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(1626..>3635)
                     /gene="prc"
                     /locus_tag="POB34_004671"
     CDS             complement(1626..>3635)
                     /gene="prc"
                     /locus_tag="POB34_004671"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HDI5921494.1"
                     /translation="LAIAGQTFAVEDITRADQIPVLKEETQHATVSERVTSRFTRSHY
                     RQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGDELRSGKLDVFYDL
                     YNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEAELNALWDSKVKFD
                     ELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAFAREIDPHTNYLSP
                     RNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAISVGDKIVGVGQTG
                     KPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLTRERIRLEDRAVKM
                     SVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDLRSNGGGALTEAVS
                     LSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFSASASEIFAAAMQD
                     YGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTIQKFYRVNGGSTQR
                     KGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTAFGPELLKEHNARI
                     AKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRLARLNERFKREGKP
                     ELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK"
BASE COUNT          835 a          987 c          866 g          947 t
ORIGIN      
        1 tccctgccct tttacctgca aaccgtgctc gggcgtagtg aagtcgaaac aggtttactt
       61 ctgacaccgt ggccgttagc aacgatggtg atggctccgc tggcaggcta tttgattgaa
      121 cgcgtacatg caggattgct gggtgcttta gggttgttca tcatggctgc ggggcttttt
      181 tccctggttc tgctgcccgc gtcacctgcg gatatcaata ttatctggcc gatgatctta
      241 tgtggcgctg gatttggctt attccagtca cccaataacc acaccattat tacctccgct
      301 ccgcgcgaac gtagcggtgg ggccagtggc atgttaggaa cggctcgtct actgggtcag
      361 agtagtggcg cggcgctggt ggcgctgatg ctaaatcagt tcggtgataa tggtacgcac
      421 gtttcgctga tggctgcggc tattctggca gtgattgctg cctgtgtgag tggtttacgt
      481 atcactcagc cacgatccag ggcataaaaa aagcgcgtcg atcaggacgc gctttttagt
      541 atttacttca tattacttca ggtattcacc cgtacgcaga gcttcaattc gtttatccag
      601 cggcgggtga gtcatgaaca actcactgag cgatttcgac ttaccgttaa tgcagaaagc
      661 catcatgctg gttgcttctt gcggttcata gctggttttc aggcgctgca gcgcggcaat
      721 cattttctcg cgaccaacca gttttgccga accggcatca gcatggaatt cacgatgacg
      781 cgagaaccac atggtgataa tgctcgcaag aataccaaac accagttcca gaaccgttgc
      841 aaccgcaaag tagatcagcg ggttgccgtt gctctcttca ccttcatcac gatttccgcc
      901 cataaaaccc gcggcaagct gcgccagaat acgggaaata aagataacga aggtgttcac
      961 cacgccctga atcagcgtca tggtgaccat atcaccattg gcgatgtggc tgatttcgtg
     1021 agcaattacc gcctcggctt catccgggct catgttctgc agcaaaccgg tgctgacagc
     1081 aaccagagag gcatcacggc gcgcaccggt tgcaaaagcg ttgatgtccg gcgcatggta
     1141 gatagccact tgcggcatag cgatccctgc ctgacgagcc tgggttgcta cagtattaac
     1201 cagccaacgt tccctttcgt tacgcggttg ctcgatcact tccccgccaa cagatcgtaa
     1261 tgccatccat ttggacatca gaagcgaaac gaaggaacca ccaaaaccga acagcaaggc
     1321 catgatcatc agcccctgaa cgctgctcga ctgtatccct gtcaggctca gtaccagccc
     1381 gaaaacgacc attacggcca ggttcgttag caggaagagc gcgattcgca tcataatttt
     1441 ctttttacct cagtttaaca aaacgcaata tgcgataccc acatcgtatg ggttacgcga
     1501 ctattttcaa gtctggatag tgcgtaagtc accagaaaga cacaacttta cattttgtag
     1561 catctgattt acggcatctt gccgctgtta aaaaatcagg cacaatttct tgtgcctgat
     1621 tgatattact tgacgggagc gggttgttcc gcgggtctgg ctttttcaag cttcgccaga
     1681 tcgagtgcga tattcaccgt ctcatccaga taaggatccg gctcctggta atctttaggt
     1741 agatcatcca gcttcttcaa ctccggttta ccttcgcgtt taaagcgttc gttcaaacgc
     1801 gccagacgcg tcgcatcatc ttcattattc tctttctcac gcacagcgta gttcagagaa
     1861 acgatattgc gcttgtcctt catagcgttg aagcgcgcga tatccttcat gatgttctgg
     1921 aactcaggat ctttcgcgat acgcgcatta tgttccttca gcagctccgg cccaaaggcc
     1981 gttaaatcgc ctgatttcac ataagtcgcg gcatcaatgc tatcccacgg cagcgcgtta
     2041 tcttcgaatt tctcacccgt ttccgtttct tcattacccg tcggcatgat gatgtctggc
     2101 gttacgcctt tacgttgcgt actgccgccg ttaacgcgat agaatttctg gatcgtgtac
     2161 tgcacagaac ccagcgctgg ccattcagga cgtaacatct gatcgtaaat acggttcaat
     2221 gaacggtatt gctgaacggt gcctttacca aacgtcggtt cacccacaac cagcgcacga
     2281 ccgtaatcct gcattgccgc ggcaaagatt tctgaagccg aagcactgaa acggtcaacc
     2341 agtaccacca gcgggccttt atagaacacc tgcccgtcgg tatcgctgtc ttcacgaacc
     2401 ttgccgttgt tatcgcggac ctgaacaatg ggacccgcag gaataaacag acctgagagc
     2461 gataccgctt cggtcagcgc cccaccgcca ttgctacgca ggtcgatgat cacactgctg
     2521 acattctgtt tttccagttt ctgcagttga actttgacat cgtctgtcaa acccacatag
     2581 aagcccggaa tatccagcac gccgactttc tctttaccga cggtcttcac cgacatttta
     2641 accgcgcggt cttcgagacg aatacgttca cgggtcaatg ttacagtgcg ggtcttggtc
     2701 cctttaccag caggtaaaat ttccagacga actttactgc ccttcggccc tttaattaag
     2761 gcaaccacat catcaagacg ccagccaatc acgtcaacca tcggcttgcc tgtttgacca
     2821 acaccgacaa ttttgtcacc aacgctgata gctttactct tcgctgccgg accacctgcc
     2881 accatcgaat tgataacggt gtagtcatca tccatttgca gcactgcgcc aataccttcc
     2941 agcgacaaac tcatttcagt gttgaactgt tcggtattac gcggggaaag atagttggta
     3001 tgcgggtcga tttcacgcgc aaacgccgtc attgccagcg agaaaacatc ttcgctgttg
     3061 gtttgcgcca gacgacgaat ggcaaatttg tagcggcgtg tcagggtttc acgaatttct
     3121 ttatccgttt ttcctgccag cttcaggctt aactcgtcga atttgacttt actgtcccac
     3181 agcgcgttca actcagcctc gtttttcggc cagggcgctt tgctgcggtc aaggttataa
     3241 gtgtcgttgc cggtgaaatc catcggcttt tccagtaccg acaaagcgta ctggtaacgc
     3301 tcaaaacggc gcttttgcgc cagattgtag agatcgtaga aaacgtcgag tttgcctgaa
     3361 cgcagttcat cgcctaactc ggttttcttt ttcgcgaact gttcaacatc gcttgccagc
     3421 agcacgttgt ggctgtaatc gagcagattc aggtagcggt caaagatttt ggccgaaaat
     3481 gcctgatcga ggtcgaactg gcgataatga gaacgggtga agcgcgacgt tacgcgctca
     3541 cttaccgtcg catgctgcgt ctcttccttt aataccggaa tttgatcagc acgcgtgata
     3601 tcttctacag cgaaggtctg gcctgctatt gcaag
//
LOCUS       DAMZET010000284         3614 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.284, whole genome shotgun sequence.
ACCESSION   DAMZET010000284 DAMZET010000000
VERSION     DAMZET010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.284"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..997
                     /locus_tag="POB34_004672"
     CDS             <1..997
                     /locus_tag="POB34_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDI5921495.1"
                     /translation="DVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFN
                     FFINQPLVRKLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTV
                     LVVQDPFTSYYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKT
                     ADFLNRMAKLGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPV
                     ATVSGESWYFFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKN
                     HKNSLGIYELSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            994..1404
                     /gene="menI"
                     /locus_tag="POB34_004673"
     CDS             994..1404
                     /gene="menI"
                     /locus_tag="POB34_004673"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HDI5921496.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            1504..1692
                     /locus_tag="POB34_004674"
     CDS             1504..1692
                     /locus_tag="POB34_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HDI5921497.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            2241..2609
                     /gene="sufA"
                     /locus_tag="POB34_004675"
     CDS             2241..2609
                     /gene="sufA"
                     /locus_tag="POB34_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HDI5921498.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            2618..>3614
                     /gene="sufB"
                     /locus_tag="POB34_004676"
     CDS             2618..>3614
                     /gene="sufB"
                     /locus_tag="POB34_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDI5921499.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSC"
BASE COUNT          890 a          856 c          965 g          903 t
ORIGIN      
        1 tgatgtgccg gagtttcgct ctcgttttct gcagctctat cacacccgtt atttacgccc
       61 gctgcgcgac cacctcgtcg ctacggtcga gagctacgcg ccgctgatgg cacgcgcgcc
      121 gaaaaccttt aacttcttca ttaaccagcc gctggtgcgc aaactctcgg aaaaacatat
      181 cggcatggtt gatttgccgc tgctgtcggt cccctcgcta caacaacaaa tggtggggca
      241 tcgctcggca aacatgacgc tggaacagct tgaatctctc aatgcagagc agaaagcgcg
      301 cacagtgttg gtggtgcagg acccctttac cagctattac gatgcgcaag tggtggcgga
      361 ttttgtccgt ctggtcgaaa aattaggttt ccagcctgtg ttactgccat tttcgccaaa
      421 tggcaaagcc cagcatatta aaggttttct taatcgtttt gcgaagacgg cgaaaaagac
      481 ggcggatttc ctcaaccgta tggcgaagct gggtatgcca atggtgggcg tcgatccggc
      541 gctggtactt tgttatcgcg atgaatataa actggcgctg ggcgaggaac gtggcgagtt
      601 taacgtctta ctggcgaatg aatggctggc aagcgcactt gagtcacagc cagtggctac
      661 agtcagcggt gaatcatggt atttctttgg tcactgtacc gaagttaccg ccttgccggg
      721 tgcgccagca caatgggccg cgatatttgc ccgttttggc gcgaaactgg aaaatgtcag
      781 cgtgggttgc tgcggcatgg cagggactta cggacatgaa gcgaaaaacc ataaaaattc
      841 gctcgggatc tatgagttat cctggcatca ggctatgcag cgactgccgc gtaaccgctg
      901 tctggcgacc ggatattcct gccgtagcca ggtaaaacgg gttgaaggca cgggggtacg
      961 ccatcctgtg caggctttac tggagattat taaatgatat ggaaacggaa aatcaccctg
     1021 gaagcactga atgctatggg tgaaggaaac atggtggggt tcctggatat tcgctttgaa
     1081 catattggtg atgacaccct tgaagcgaca atgccagtag actcgcggac aaagcagcct
     1141 ttcgggttgc tgcatggagg agcatccgtg gtactggccg aaagtatcgg ttccgttgcc
     1201 ggttatttat gtaccgaagg tgagcaaaaa gtggttggtc tggaaatcaa tgctaaccac
     1261 gtccgctcgg cacgagaagg gcgggtgcgc ggggtatgca aaccgttgca tctcggttcg
     1321 cgtcaccagg tctggcagat tgaaatcttc gatgagaaag ggcgtttgtg ctgttcgtca
     1381 cgattgacga ccgccatttt gtgacgttgt caccagaaaa gtgtgacgca tattgctttt
     1441 ggtcattatc tgcacagtaa acggtggtat actgtgcagg tcttgtctaa aagcgaggat
     1501 gctatgtcta ctcaacttga tcccacccag ctggccattg aatttttacg ccgtgaccag
     1561 agcaatcttt cacctgccca atatctcaaa cgtctgaaac aactagaact tgaatttgct
     1621 gacctgctta ctctctcttc tgctgaatta aaagaagaaa tctactttgc ctggcgtctg
     1681 ggcgtgcatt gatcctgact taataccgca agttcgccaa atcgacctgg attaacctcc
     1741 gtccaggttc gatgaccttc ttaatactgc ttatctaccc gcctgatatt gttcaaaaat
     1801 aatactaatc gcagtttgtg ttaaaacggc gggttagctt tatgagatta ttcttatcgc
     1861 cccttcaaga gctaagccac tgagagtgcc ggagataagc gccggatggg gtagaaaccc
     1921 ttaagcctgt gtcgcactga cttaagggtt ttcttatttc tgaacgctgc acttttttac
     1981 ctatctctta aatagtcatt ttcaatacaa ctttttcttt ttcatcacag ccttaatgct
     2041 ccttagataa taaccattat ctaacaatga gatacctaat tcttagcgtg cctgttaacc
     2101 cacacatcag ggtctatgct tattaaaagc aggcaaaaag catgttttac ggtaaagccc
     2161 ctgcgtttgc tgggttgaac tgataatcat tatcactaac atgctgttat acgctgaaag
     2221 cgatgaagtg aggtaaatag atggacatgc attcaggaac ctttaaccca caggatttcg
     2281 cctggcaagg cttaacgctg acacccgcag cggcgataca catccgcgag ctggtggcaa
     2341 agcagccagg tatggtcggc gtgcgcttag gtgtgaagca aacgggctgc gcgggctttg
     2401 gctatgtgct cgacagtgtt agcgagccgg acaaagacga tctgctgttt gaacacgacg
     2461 gcgcgaagct gtttgtcccg ctgcaagcga tgccgtttat tgatggcacg gaagtcgatt
     2521 tcgttcgtga aggacttaat cagatattca aatttcacaa ccctaaagcc cagaatgaat
     2581 gtggctgtgg cgaaagcttt ggggtatagg cggtactatg tctcgtaata ctgaagcaac
     2641 tgacgatgtc aaaacctgga ccggcggccc gctgaattat aaagaaggat tcttcaccca
     2701 gttagccacc gatgagctgg caaaggggat aaacgaagag gtggtgcgcg caatttcggc
     2761 gaagcgtaat gagccggagt ggatgctgga gtttcgtctc aacgcctatc gcgcatggct
     2821 ggagatggaa gaaccgcact ggttgaaagc gcactacgac aagctgaatt atcaggatta
     2881 cagctactac tcagcaccat cgtgcggtaa ttgtgacgac acttgcgcgt ctgaacctgg
     2941 cgcggtgcag caaactggcg cgaacgcctt tttaagtaaa gaggtggagg cggcgtttga
     3001 gcagttgggc gttcccgtgc gggaaggcaa agaggtggcg gtggatgcca ttttcgactc
     3061 agtttcggtt gccactactt atcgcgaaaa actggcggag cagggaatta ttttctgttc
     3121 ctttggtgag gcgatccacg atcacccgga actggtgcgt aaatatctcg gcaccgtggt
     3181 gccggggaat gacaacttct ttgccgcgct taatgcggcg gtagcctctg acggtacgtt
     3241 tatttatgtg cctaaaggcg tgcgctgccc gatggaactt tccacctatt ttcgcattaa
     3301 cgcggaaaaa accgggcagt ttgagcgtac cattctggtg gccgacgaag acagctacgt
     3361 cagctacatt gaaggctgtt ccgctccggt gcgtgacagc tatcagttac acgcggcagt
     3421 ggtggaagtc atcatccata aaaacgccga ggtgaaatat tccacggtac aaaactggtt
     3481 ccctggcgat aacaacaccg gcggtattct caacttcgtc accaagcgtg ctttgtgcga
     3541 aggcgaaaac agcaaaatgt catggacgca atcagaaacc gggtcagcga ttacgtggaa
     3601 atatcccagc tgca
//
LOCUS       DAMZET010000285         3607 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.285, whole genome shotgun sequence.
ACCESSION   DAMZET010000285 DAMZET010000000
VERSION     DAMZET010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.285"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1741
                     /locus_tag="POB34_004677"
     CDS             <1..1741
                     /locus_tag="POB34_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HDI5921500.1"
                     /translation="IAAEAEKAGMNPVDYTVKSLKEGSIRFAAEQPENGKNHPRNLFI
                     WRSNLLGSSGKGHEFMLKYLLGTEHGIQGKDLGQQGGVKPEEVDWQDNGLEGKLDLVV
                     TLDFRLSSTCLYSDIILPTATWYEKDDMNTSDMHPFIHPLSAAVDPAWEAKSDWEIYK
                     AIAKKFSEVCVGHLGKETDIVTLPIQHDSAAELAQPLDVKDWKKGECDLIPGKTAPHI
                     MVVERDYPATYERFTSIGPLMEKIGNGGKGIAWNTQSEMDLLRKLNYTKAEGPAKGQP
                     MLNTAIDAAEMILTLAPETNGQVAVKAWAALSEFTGRDHTHLALNKEDEKIRFRDIQA
                     QPRKIISSPTWSGLEDEHVSYNAGYTNVHELIPWRTLSGRQQLYQDHQWMRDFGESLL
                     VYRPPIDTRSVKEVIGQKSNGNPEKALNFLTPHQKWGIHSTYSDNLLMLTLGRGGPVV
                     WLSEADAKDLGIADNDWIEVFNSNGALTARAVVSQRVPAGMTMMYHAQERIVNLPGSE
                     ITQQRGGIHNSVTRITPKPTHMIGGYAHLAYGFNYYGTVGSNRDEFVVVRKMKNIDWL
                     DGEGNDQVQESVK"
     gene            1738..3276
                     /gene="narH"
                     /locus_tag="POB34_004678"
     CDS             1738..3276
                     /gene="narH"
                     /locus_tag="POB34_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDI5921501.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            3273..>3607
                     /gene="narJ"
                     /locus_tag="POB34_004679"
     CDS             3273..>3607
                     /gene="narJ"
                     /locus_tag="POB34_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDI5921502.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELP"
BASE COUNT          902 a          934 c         1023 g          748 t
ORIGIN      
        1 tatcgctgcc gaagcggaaa aagccgggat gaatccggtg gactatacgg tgaaatccct
       61 gaaagagggt tccatccgtt ttgcggcaga acaaccagaa aacggtaaaa accacccgcg
      121 caacctgttc atctggcgtt ctaacctgct cggttcttcc ggtaaaggtc atgagtttat
      181 gctcaagtac ctgctgggga cggagcacgg tatccagggt aaagatctgg ggcaacaggg
      241 cggcgtgaag ccggaagaag tggactggca ggacaatggt ctggaaggca agctggatct
      301 ggtggttacg ctggacttcc gtctgtcgag cacctgtctc tattccgaca tcattttgcc
      361 gacggcgacc tggtacgaaa aagacgacat gaatacttcg gatatgcatc cgtttattca
      421 cccgctgtct gcggcggtcg atccggcctg ggaagcgaaa agcgactggg aaatctacaa
      481 agccatcgcg aagaaattct ccgaagtgtg cgtcggccat ctgggtaaag aaaccgacat
      541 cgtcacgctg cctatccagc atgactctgc cgctgaactg gcgcagccgc tggatgtgaa
      601 agactggaaa aaaggcgagt gcgacctgat cccaggtaaa accgcgccac acattatggt
      661 cgtagagcgc gattatccgg cgacttacga acgctttacc tctatcggcc cgctgatgga
      721 gaaaatcggt aatggcggta aagggattgc ctggaacacc cagagcgaga tggatctgct
      781 gcgtaagctc aactacacca aagcggaagg tccggcgaaa ggccagccga tgctgaacac
      841 cgcaattgat gcggcagaga tgatcctgac actggcaccg gaaaccaacg gtcaggtagc
      901 cgtgaaagcc tgggctgccc tgagcgaatt taccggtcgt gaccatacgc atctggcgct
      961 gaataaagaa gacgagaaga tccgcttccg cgatattcag gcacagccgc gcaaaattat
     1021 ctccagcccg acctggtctg gtctggaaga tgaacacgtt tcttacaacg ccggttacac
     1081 caacgttcac gagctgatcc catggcgtac gctctctggt cgtcagcaac tgtatcagga
     1141 tcaccagtgg atgcgtgatt tcggtgaaag cctgctggtt tatcgtccgc cgatcgacac
     1201 ccgttcggtg aaagaagtga taggccagaa atccaacggc aacccggaaa aagcgctcaa
     1261 cttcctgacg ccgcaccaga agtggggtat ccactccacc tacagcgaca acctgctgat
     1321 gctgacttta ggtcgcggtg gtccggtggt ctggttgagt gaagccgatg ccaaagatct
     1381 gggtatcgcc gataacgact ggattgaagt attcaacagc aacggtgctc tgactgcccg
     1441 tgcggttgtc agccagcgtg ttccggcagg gatgaccatg atgtaccacg cgcaggaacg
     1501 tatcgttaac ctgcctggtt cggaaattac ccaacagcgt ggtggtatcc ataactcggt
     1561 cacccgtatc acgccgaaac cgacgcatat gatcggcggc tatgcccatc tggcatacgg
     1621 ctttaactac tacggcaccg taggttctaa ccgcgatgag tttgttgtag tgcgtaagat
     1681 gaagaacatt gactggttag atggcgaagg caatgaccag gtacaggaga gcgtaaaatg
     1741 aaaattcgtt cacaagtcgg catggtgctg aatctcgata agtgcatcgg ctgccacacc
     1801 tgttcagtta cctgtaaaaa cgtctggacc agccgtgaag gcgtggaata cgcgtggttc
     1861 aacaacgtgg aaaccaagcc gggtcagggc ttcccgactg actgggaaaa ccaggaaaaa
     1921 tacaaaggcg gctggatccg taaaatcaat ggcaaactgc agccgcgcat gggtaaccgc
     1981 gccatgctgc tgggtaaaat cttcgctaac ccgcatctgc cggggatcga cgattattac
     2041 gagccgttcg attttgatta tcagaacctg cataccgcgc cggaaggcag caaatcgcag
     2101 ccgattgccc gtccgcgttc gctgattacc ggggaacgga tggcgaaaat cgaaaaaggg
     2161 ccgaactggg aagatgacct gggtggtgag tttgacaaac tggcgaaaga caagaacttc
     2221 gacaacatcc agaaggcgat gtatagccag ttcgaaaaca ccttcatgat gtatttgccg
     2281 cgcctgtgcg aacactgcct gaacccggca tgtgtggcga cctgcccgag cggtgcgatt
     2341 tacaagcgtg aagaagatgg catcgtcctg atcgatcagg ataaatgccg tggctggcgt
     2401 atgtgcatca ccggatgccc atacaaaaaa atctacttca actggaagag cggtaagtct
     2461 gagaagtgca tcttctgcta tccgcgtatt gaagcgggtc agccgaccgt gtgctcagaa
     2521 acctgtgtcg gtcgtatccg ttatctcggc gtgctgttat acgatgccga cgcgattgaa
     2581 cgtgcagcca gcaccgagaa cgagaaagat ctttaccagc gtcagctgga cgtgttcctc
     2641 gatccgaacg atccgaaagt catcgagcag gcgattaaag acggtattcc gctgagcgtt
     2701 attgaagctg cacagcagtc gccggtttat aaaatggcaa tggaatggaa actggcgctg
     2761 ccgctgcatc cagaatatcg cacactgccg atggtctggt acgtgccgcc tctgtctccg
     2821 attcagtctg cagcagacgc gggtgagctg ggtagcaacg gcattctgcc agacgtcgaa
     2881 agcctgcgta ttccggtaca gtatctggcg aatctgctga ccgccggtga taccaaaccg
     2941 gtactgcgcg cgctgaaacg tatgctggcg atgcgtcatt acaaacgtgc tgaaaccgtt
     3001 gacggtaaag ttgatacccg tgcgctggaa gaggtcggtc tgaccgaagc ccaggcacag
     3061 gagatgtacc gttatctggc gattgctaac tacgaagatc gctttgtggt gccgagtagt
     3121 catcgtgaac tggcacggga agccttcccg gagaaaaatg gctgcggctt tacctttggt
     3181 gatggctgcc acggttcaga taccaaattc aatctgttca acagccgtcg tatcgatgcc
     3241 atcgatgtga ccagcaaaac ggagccgcat ccatgatcga actcgtgatt gtatcgcgtc
     3301 tccttgaata tccggatgct gccttatggc agcatcaaca agagatgttt gaggcgattg
     3361 ccgcgtcgaa aaatctgcca aaagaggatg cccatgcgct gggcattttc ctgcgcgatt
     3421 taacggcgat ggatccgctc gatgcccagg cccagtacag cgaactgttc gaccgtggcc
     3481 gcgccacgtc actgttgctg tttgaacatg tgcacggcga atcccgcgac cgcggccagg
     3541 cgatggtgga cctgctggcg cagtacgagc agcacggctt gcagttaaac agccgcgaat
     3601 tgccgga
//
LOCUS       DAMZET010000286         3589 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.286, whole genome shotgun sequence.
ACCESSION   DAMZET010000286 DAMZET010000000
VERSION     DAMZET010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.286"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(88..1269)
                     /gene="nimT"
                     /locus_tag="POB34_004680"
     CDS             complement(88..1269)
                     /gene="nimT"
                     /locus_tag="POB34_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HDI5921503.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     FNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            1366..2187
                     /gene="nimR"
                     /locus_tag="POB34_004681"
     CDS             1366..2187
                     /gene="nimR"
                     /locus_tag="POB34_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HDI5921504.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTHRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            complement(2144..2590)
                     /locus_tag="POB34_004682"
     CDS             complement(2144..2590)
                     /locus_tag="POB34_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDI5921505.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            2760..2864
                     /locus_tag="POB34_004683"
     CDS             2760..2864
                     /locus_tag="POB34_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921506.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            complement(2865..3368)
                     /gene="yeaK"
                     /locus_tag="POB34_004684"
     CDS             complement(2865..3368)
                     /gene="yeaK"
                     /locus_tag="POB34_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HDI5921507.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(3411..>3589)
                     /locus_tag="POB34_004685"
     CDS             complement(3411..>3589)
                     /locus_tag="POB34_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921508.1"
                     /translation="AAQLPERIEKRLQHIAPQKEIGFSSGIYAMKENDTLHDAYKASD
                     ERLYVNKQNKNSRS"
BASE COUNT          968 a          897 c          902 g          822 t
ORIGIN      
        1 cggatcataa acgcgtttgc actgaatatt catcgtgttt gttctccctt tcacgcatga
       61 tttgtcacgt cactatagca ccggatatta gcgaatttct ttgtctcttc cggcgcaaag
      121 tccgaaaatc gccatcagta gtgaaagtat tgcaacaccc attagtggta cggaccagtc
      181 accgttagca tcgtgtattt tacccatcag cggcggccca caggctgcca gcaaataccc
      241 gacggattgt gccatccccg agagtgccgc cgcctgatgc gcagaacttg cccgaagacc
      301 aatgaacgtc aaccccagta tcattgttgc gccggaacca aaaccgaaaa gcagagtcca
      361 ggtgatcgcg tgtgctggca taaagcagag cccaaccgcg cccactgcgc acatcaaggc
      421 aacgaacgct gcaataccac gctgatcttt cacatgatgt aagaaaagtg ggatcagcaa
      481 accgggagct gctgtggcta gttgcagcaa accatgcagt gaacccgcct gtgcttcgct
      541 atagccgtga ctgatgagga tcgccggaag ccagccaata atcacgtaat agaccagtga
      601 gttgatccca agaaacaatg tgacctgcca ggcaagcggc gaacgccaga taccccgagt
      661 atgtaaggcg cgcgaggtac tcaaatttgc atgttgttgg cttcgccact gtggcagcca
      721 taaaaaaaga gccagcagag gaaaacacat cagcatgagc aacgcgcctt gccagccaaa
      781 accgttaaaa gccagcggca ctaccatagc cgatccaagt gccgccgcag ctcccattgt
      841 cagggaatat gcgccggtaa gtctggcgac ggaatgaggg aaatcgcgtt taattaatcc
      901 tggcagtaag acattaccta atgcaatccc accaccaatg accgctgtac cgccaaataa
      961 taagtaaggc gaagggagag agcgaattgc gataccagca cagatcaaaa gtaacgcggc
     1021 aaacaggcta cgttccatac caaaacgtcg cgctaccggg gcggccaaag gtgagattag
     1081 cgcaaaggcc aataatggca gggtggtcaa taagccggtt tgcgctgtcg tcagcgagta
     1141 agcggaacga atcgtatcca gtaacggtgc tgcgccggta aaggtgacgc gtaatgttgt
     1201 ggcaatcatc agaatgccag cgataaggac aatcctgttt ttgccgctta atgaagttga
     1261 acaggtcata tgcttctcat tgttacaaac gaggctgtac cttaccgttt ttattgttat
     1321 gttgaatcaa gctaaaatga caaattatcg ctaaattcgg acaaaatgat gcacaggttg
     1381 aatctcaatg gctatgaacc cgaccgacac catgaagccg cggtcgcatt ttgtattcac
     1441 gccggaacgg atgagttaac cagtcctgta catcagcacc gcaaaggaca gctgatactg
     1501 gcgctacatg gtgcaattac ctgtacggtg gaaaatgctt tgtggatggt gccgccgcaa
     1561 tatgcggttt ggataccggg cggggtggaa catagcaatc aagtgacagc taacgctgaa
     1621 ctctgttttc tgtttatcga acccagcgca gtgacaatgc cgacaacatg ttgcacattg
     1681 aaaatatcgc ccttatgccg ggaattgatt ttaaccctgg caaacaggac gacgactcag
     1741 cgtgctgagc cgatgaccca cagattaatt caggtcctgt ttgatgaact ccctcagcag
     1801 ccacaacaac agctgcacct gcccgtttct tctcatccta aaatccgcac gatggtggag
     1861 atgatggcga aagggcccgt cgagtggggg tcattagggc aatgggctgg cttttttgcg
     1921 atgagtgaac gcaatcttgc gcgactgata gtaaaagaga cggggttgag ttttcgccag
     1981 tggcgtcagc agttacagct gattatggca cttcagggat tagtgaaagg cgatactgta
     2041 cagaaagttg ctcatacgct ggggtatgat tcaacaacgg cctttattac tatgtttaag
     2101 aaaggcctgg ggcaaacgcc aggccgatat attgcgggat taactactgt ttccccacaa
     2161 tcagcgaaac cagacccgcg gcaataagcg ggccgaccgg tacgccgcga aacagcgcca
     2221 cgcctaaaac agtgcctacc agcagcccgg cgaccagttg cggctggctg cccattaagg
     2281 tcacgccgcg cccacccagc caggagacaa tcacgcctac tgcaattgcc accagcgatt
     2341 tccagttcag aaacgagtga atcaaggttg agggcggtag cgtcccgctg gcgatggggg
     2401 ccataacgcc aatcgtcaga atgattatcc cgatacttag cccctgtttt tcaatccacg
     2461 ggaaaaaggt gcttaacggt gtgacgcgga caatgatcaa caccagaatt gaaacggcga
     2521 cagtggtgtt atgactgata aagcccagcg cagctaaccc gagcaggatc agcaaagtga
     2581 catcaaacat cgtggtttcc ttgccaaaaa ataataagcc tgcttacttt acgcttgctg
     2641 caggaaggaa gtagaaaatc tttacgtttt ttgcataaaa aatcaggcga actgtcatca
     2701 aactgccatt tgtgcacgct aacaataacg tgaaaacagc tatctcccgg gggcatgcta
     2761 tgaacgatca aatgttcgtc gagacactga ttatcacgtc atcgtttttt gccattgctg
     2821 ttgtgctggt tttgtccgtt ctgcttattg aacggacagg ctagttacgc agtgcggcgg
     2881 aaattgagca gttctggttg cgcaatgcgc aaataatccg tggttttcaa aataacggat
     2941 ttatccagca tccctgcatt gaaggcgatt tcatcaaatc gttcgaagag tagagggtcg
     3001 gcaaccagtt tgagttttgg atggaagctg aaagggggga tcgcgccgaa gacacagcct
     3061 gtgagttcat cgacttccgc cgggctggcc aacgaagcgc gtaatccgcc tatatggctg
     3121 gcgagttggc tcagatcggc ctgttgatcg gcagcgagga tcgccagaac atgttgattt
     3181 acgccattgc ctttgacttt acataccagt gccttagctc cctggcctaa agcggtgcca
     3241 cgaatttctg acaccgcttc acatttacct accgcttcat gggtaacgac gcggaagtta
     3301 gcaccttctt gtgataataa agcgattaac cgctgatgcg tcacgcttcc tttagccatt
     3361 tcagtcattt cacgctcctg agattacaag caaacaacca cagaaggtta tcatgaacgg
     3421 ctgtttttgt tctgcttatt gacatataaa cgctcatcgg aagctttata cgcatcatgt
     3481 aacgtatcgt tttctttcat cgcgtaaata ccggaagaga agccgatctc tttctgcggc
     3541 gcgatatgtt gcagacgttt ttcgatacgt tcaggcagtt gtgctgcaa
//
LOCUS       DAMZET010000287         3566 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.287, whole genome shotgun sequence.
ACCESSION   DAMZET010000287 DAMZET010000000
VERSION     DAMZET010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.287"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1457
                     /gene="sapA"
                     /locus_tag="POB34_004686"
     CDS             <1..1457
                     /gene="sapA"
                     /locus_tag="POB34_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HDI5921509.1"
                     /translation="QFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRRDVPFQ
                     KTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSVRKLDN
                     HTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPYQLSEY
                     RAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAASQLSIL
                     RDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYGTAETA
                     ASILPRASWAYDNEAKITEYDPAKSREQLKSLGLENLTLKLWVPTRSQAWNPSPLKTA
                     ELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRPLLSCA
                     AIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLASSLRL
                     QAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            1454..2419
                     /gene="sapB"
                     /locus_tag="POB34_004687"
     CDS             1454..2419
                     /gene="sapB"
                     /locus_tag="POB34_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HDI5921510.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            2406..3296
                     /gene="sapC"
                     /locus_tag="POB34_004688"
     CDS             2406..3296
                     /gene="sapC"
                     /locus_tag="POB34_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HDI5921511.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSIVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            3296..>3566
                     /locus_tag="POB34_004689"
     CDS             3296..>3566
                     /locus_tag="POB34_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI5921512.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHN"
BASE COUNT          716 a          937 c          983 g          930 t
ORIGIN      
        1 cccagtttta tgatcgactg ctggatgtcg atccctatac ctatcgcctg atgccggaac
       61 ttgccgaaag ctgggaagta ctcgacaacg gcgcgaccta tcgcttccac ctgcgtcgcg
      121 atgttccgtt tcaaaaaacc gactggttta ctcccactcg taaaatgaat gccgacgatg
      181 tggtgtttac cttccagcga atttttgacc gcaacaaccc gtggcataac gtcaacggca
      241 gcaacttccc ctacttcgac agcctgcaat ttgccgataa cgtgaaaagc gtccgcaaac
      301 tggataatca taccgttgag ttccgactcg cccagccgga tgcttctttt ttgtggcacc
      361 tcgcaaccca ttatgcttcg gtcatgtcgg cagaatatgc ccggaagtta gagaaagaag
      421 atcgccagga gcaactcgac cgtcaaccgg tcggcaccgg tccgtatcaa ttgtcggaat
      481 accgcgccgg gcaatttatt cgcctacaac gtcatgatga cttctggcgc ggtaaaccgt
      541 taatgccgca ggtggtggtg gatttaggct ccggcggcac cggacgtctg tcgaaactcc
      601 tgactggtga atgcgacgtt ctggcctggc ctgctgccag ccagctatcc attttgcgtg
      661 acgacccgcg cttgcgttta acgctgcgtc ccgggatgaa cgtcgcctat ctggcattta
      721 acaccgccaa accgccgcta aataatcccg ctgtccgcca tgcgctggca ctggcgatta
      781 ataaccagcg cctgatgcaa tccatctatt atggtacggc tgaaacggcg gcctctattt
      841 taccgcgcgc ctcgtgggcc tatgacaacg aggctaaaat tactgaatac gatccggcga
      901 aatcgcgcga acagttgaag tcgttggggc tggaaaattt aacgctgaaa ctgtgggtgc
      961 ccacacgttc gcaggcgtgg aaccccagtc cactgaaaac tgccgaactg attcaggcgg
     1021 atatggcgca ggttggcgta aaagtggtga ttgtgccggt agaaggtcgc tttcaggagg
     1081 cgcggttgat ggatatgagc catgatctga cgttatccgg ttgggcgacg gacagtaacg
     1141 acccggacag tttcttccgt cctttactga gctgcgcggc aattcattca cagaccaacc
     1201 tcgcccactg gtgcgatccg aaattcgaca gcgtgttgcg taaggcgctc tcctcgcagc
     1261 agctggcggc gcgtattgaa gcctatgacg aagcgcagag tattctggcg caggaattgc
     1321 ccattttgcc gctggcgtcg tcattgcgtt tgcaggccta tcggtacgat atcaaaggtc
     1381 tggtacttag cccgtttggt aacgcctcct ttgctggggt gtatcgcgag aaacaggatg
     1441 aggtgaaaaa accatgatta tcttcacctt acgccgcatt ttgttattga ttgtcacctt
     1501 gttcctgctg accttcgttg gcttcagctt gagctatttc acccctcatg cgccgttaca
     1561 aggcgcgtca ctgtggaatg cctgggtgtt ctggtttaac ggcctgatcc actgggattt
     1621 tggtgtttcc agcattaacg gtcaaccgat tgccgaacag ttaaaagagg tcttcccggc
     1681 gacgatggaa ctgtgcatcc tcgccttcgg ttttgcgctg attgttggca tccccgtggg
     1741 gatgattgcc gggattacgc gccataagtg gcaggataac ttgatcaacg ccatcgcctt
     1801 actgggcttt tcaatcccgg tgttctggct ggcgcttctg ttgacgctgt tttgttcact
     1861 cacgctaggt tggttgcccg tttcagggcg tttcgatctg ctctacgaag tgaaaccgat
     1921 taccggtttt gcgttgattg atgcctggct ttcggactca ccatggcggg atgaaatgat
     1981 catgagcgca atccgccata tgatattgcc cgtgatcacc ctgtcggttg cgccaacaac
     2041 tgaagtgatc cgcctgatgc gtatcagcac tatcgaagtg tatgaccaga actatgtaaa
     2101 agcggcggcg acccgcggtt tgtcgcgctt tactattttg cgtcgccacg ttctgcataa
     2161 cgcgttaccg ccggttattc ctcgcctggg cttacagttt tccaccatgc tgacgctggc
     2221 gatgatcacc gaaatggttt ttagctggcc gggtttaggg cgctggttaa ttaacgccat
     2281 ccgccagcag gattacgcag ccatttccgc cggagtgatg gtgtgtggct cactggtcat
     2341 tattgttaac gtgatttctg atattttggg tgccatggct aaccctctga aacataagga
     2401 atggtatgcc ttacgatagc gtatacagcg aaaagcgccc gccgggcacg ctgcgtaccg
     2461 cctggcgcaa attttatagt gacgcctctg cgatggtcgg cctgtacggc tgcgcggggc
     2521 tggctgtact gtgtattttt ggcggctggt ttgcgcctta cggcatcgac cagcaatttc
     2581 tcggttatca attattgccg ccgtcatggt cgcgctatgg cgaagtttct ttcttcctgg
     2641 ggactgacga cctcggacgc gatgttttaa gccgcttact gagcggagct gcgccaaccg
     2701 tgggtggcgc atttgtggtg acgcttgccg cgacgatctg tggcctggta ctgggcacct
     2761 tcgccggggc gacacacggc ctccgctcag cggtgcttaa ccatattctg gataccttac
     2821 tggcgatccc ttcgctgctg ttggcaatta tcgttgtagc gtttgccgga ccgagtttgt
     2881 cccacgctat gtttgctgtc tggctggcat tgctgccgcg tatggtacgt tcgatttaca
     2941 gcatagtgca tgacgaactg gaaaaagagt acgttatcgc cgcccgtctg gatggcgcat
     3001 caacgctgaa tattctctgg tttgctgtga tgccaaacat caccgccggg ctggtcaccg
     3061 agatcacccg cgcactgtcg atggcaattc tcgatatcgc cgcgctgggc tttctcgatc
     3121 tcggcgcaca actcccctcg cctgaatggg gagcgatgct cggtgatgcg ctggaactga
     3181 tttatgtcgc cccgtggact gtcatgctgc caggtgcggc aattatgatt agcgtgttgt
     3241 tggttaacct ccttggcgat ggcgttcgtc gtgcgattat tgcgggggtg gaataatgcc
     3301 attactcgat attcgtaacc tgactattga atttaaaacc ggggatgagt gggttaaagc
     3361 cgttgaccgc gtaagtatga ccttaaccga aggtgaaatc cgcggtcttg ttggcgaatc
     3421 cggttccggc aaaagtttga ttgcgaaagc gatttgtggg gtgaataaag ataactggcg
     3481 tgttactgca gaccgtatgc gttttgatga tatcgatttg ctgcgtctct ccgcacgcga
     3541 acggcgcaag ctggttggtc acaacg
//
LOCUS       DAMZET010000288         3552 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.288, whole genome shotgun sequence.
ACCESSION   DAMZET010000288 DAMZET010000000
VERSION     DAMZET010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.288"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..269
                     /locus_tag="POB34_004690"
     CDS             <1..269
                     /locus_tag="POB34_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HDI5921513.1"
                     /translation="ELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLARLEGILLD
                     PVYTGKAMAGLIDGISQKRFKGEGPILFIHTGGAPALFAYHPHV"
     gene            284..952
                     /gene="tcyL"
                     /locus_tag="POB34_004691"
     CDS             284..952
                     /gene="tcyL"
                     /locus_tag="POB34_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HDI5921514.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            949..1701
                     /gene="yecC"
                     /locus_tag="POB34_004692"
     CDS             949..1701
                     /gene="yecC"
                     /locus_tag="POB34_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HDI5921515.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATERARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            1931..2653
                     /gene="sdiA"
                     /locus_tag="POB34_004693"
     CDS             1931..2653
                     /gene="sdiA"
                     /locus_tag="POB34_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HDI5921516.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRSSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(2721..2945)
                     /locus_tag="POB34_004694"
     CDS             complement(2721..2945)
                     /locus_tag="POB34_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HDI5921517.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(2932..3108)
                     /locus_tag="POB34_004695"
     CDS             complement(2932..3108)
                     /locus_tag="POB34_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921518.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            3404..>3552
                     /locus_tag="POB34_004696"
     CDS             3404..>3552
                     /locus_tag="POB34_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDI5921519.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDV"
BASE COUNT          894 a          799 c          916 g          943 t
ORIGIN      
        1 aagaactgga gctgaccgca tcagcggaaa ttttactctg ggatgactat tttgcacctg
       61 gctacggcgt gccgaacgac gaaggcatgg aagcagtgaa attgctggcg cggctggaag
      121 gcattctgct tgatcctgtg tataccggaa aagcgatggc ggggctgatt gacggtatca
      181 gtcagaaacg cttcaaaggt gaagggccga ttctgtttat tcataccggc ggtgcgcctg
      241 cgctgttcgc ctatcatccc cacgtttagc tccgggaacg ctcatgcaag aaagtataca
      301 actggttatt gattccttgc cgttcctgct gaaaggggct gggtatacgc tgcaactcag
      361 tatcggcggc atgttttttg gcttactgct cgggtttatt ctcgcgctga tgcgcctgtc
      421 gccaatctgg cccgtgcgct ggctggcgcg tttttatatc tctatttttc gtggtacgcc
      481 gctcatcgca caactgttta tgatctatta cggcctgccg cagtttggta tcgaattaga
      541 tcccattccg tcagcaatga ttggtctgtc gctcaacact gccgcctatg ctgccgaaac
      601 gctgcgcgcc gccatttctt ctattgataa aggtcagtgg gaagccgctg ccagtattgg
      661 tatgacgcca tggcagacta tgcgtcgcgc gattttgcca caggcggcgc gggtggcgtt
      721 gccgccgctg tcgaacagct ttatcagcct ggtaaaggat acgtcgctgg ccgcgacaat
      781 ccaggtgccg gagctgttcc gtcaggcgca gttgattacc tcgcgcacgc tggaggtgtt
      841 caccatgtac ctggcggctt cgctcatcta ctggatcatg gcgactgtgt tatcgacgtt
      901 gcagaaccat tttgagaatc aacttaatcg ccaggagaga gaaccaaaat gagtgccatt
      961 gaagttaaga acctggtgaa aaaatttcac ggtcagacgg tgctgcacgg tatcgacctt
     1021 gaggtaaagc ctggcgaagt ggtggcaatt atcggtccga gtggttccgg caaaaccacg
     1081 ttgctacgca gcataaatct gctggaacaa ccggaagcgg gaactatcac cgttggcgat
     1141 atcactattg atactgcacg ttcattaagt cagcaaaaat cgctgattcg ccagttgcgt
     1201 cagcacgtcg ggtttgtctt ccagaacttt aatttgtttc cgcatcgtac ggtactggaa
     1261 aacattattg aagggccggt gatcgtcaaa ggcgaaccga aagaagaggc cacggagcgc
     1321 gctcgcgagc tgttggcgaa agttgggctg gccggtaaag aaaccagcta tccacgtcgt
     1381 ttgtctggcg gtcaacagca acgtgttgcg attgcacgtg cgctggcaat gcgtcctgag
     1441 gtgattttgt ttgacgagcc aacgtcggcg ctggatccag agctggtggg cgaagtcctg
     1501 aacaccatcc gtcagctggc gcaggaaaag cgcacgatgg tgattgtgac gcacgaaatg
     1561 agctttgccc gtgatgttgc ggaccgggcg atttttatgg accaggggcg gatagtcgag
     1621 cagggggcag caaaagcgtt atttgccgat ccccagcagc ctcgcacccg ccagttcctt
     1681 gagaagtttc tgctgcaata ataagaaata atcagccccg acgattcacc tgtcggggcg
     1741 ggacgccatt tcaagcgtga taaaactgct taacaaatca gcataactca ttaataacat
     1801 aagagaatgc gatggcttgc aaaagtaatt cattgcctga ataatataaa ttatatataa
     1861 atcttattta tgtgatagtt tgaattatca ttataaatga tactcactct caggggcgtt
     1921 gcggtttact atgcaggata cggatttttt cagctggcgt cgcacgatgc tgttgcgttt
     1981 tcagaggatg gaggccgcag aagaggtcta ccatgaaata gagcttcagg ctcagcagct
     2041 agagtacgat tactattcgt tatgtgttcg ccacccggta ccattcacgc gacctaaagt
     2101 ggctttctac accaattacc ctgaggcgtg ggttagttat tatcaggcaa aaaactttct
     2161 cgcaattgat ccggtgctga accctgaaaa ctttagtcag ggccatttaa tgtggaatga
     2221 tgacttattc agcgaagcac agccgttatg ggaagccgcg cgcgcacatg gtttacgccg
     2281 cggtgtcact cagtatttaa tgctgccaaa ccgggcgctg ggctttttgt ccttttcccg
     2341 tagcagcgcg cgcgaaatac ccattcttag tgatgaactg caattaaaaa tgcagttact
     2401 ggtgcgcgaa agtctgatgg ctctgatgcg tttaaatgat gaaatagtga tgacgccaga
     2461 gatgaatttc agcaagcgcg aaaaagaaat tttgaaatgg acggcggaag ggaagacatc
     2521 agcagagata gcgatgattt tgtcaatctc tgagaatacg gtcaatttcc accagaaaaa
     2581 catgcagaaa aaaattaatg caccaaataa gacccaggtt gcctgttacg cggccgctac
     2641 tggcttaatt tgatctcttt tctgtcctgc gtgccagatg caaaaaccgg ccgaaaggca
     2701 cgctatcagc cggttttata ttactgacgg taggcttgtt taatttgctt aaccgttttg
     2761 gaaaatactg cagcctgggt ttcgtcttcg atcagcgcaa gctgtttttc catctttaac
     2821 atcacgcggc ccgcgtcagc ttgtcccatc gcctgtagca tcagcgtcag catcgctttc
     2881 aggcaggaga cttcatttgc cagttcttga ttattctcgg cagtagaaaa atcaggcgta
     2941 ctcatttatt ttcctcgtca tgttgcaatg aaaatttgcg gtgaaaaatg ttaaggcggc
     3001 ggagtatacc ataagctttg ctaaaaatag cagtggttgt tttttgagcg tgatatcggc
     3061 agtgctataa aatacgttaa tataatgacc aataaatatt tttatcatga atgttttttg
     3121 cccgtattgt tcgggttaat taatgttaca tattcagcgg gctgattttc atttttgctg
     3181 aataaagtca atttttgtca catttcatcg tagggcttac tgtgaaacga tccggtaagc
     3241 cgttggtgac gggcgtgacc ataactgtgg acaatcgaat tgacaaaaac gagagaaaaa
     3301 tcgaataccc accattttta acgtttcaaa gttgcaataa aaaccgctaa tatacgaatg
     3361 actaactatc agtagcgtta tccctatttc tggagatatt cctttgatca acgttctact
     3421 tgttgatgac cacgaactgg tgcgcgcagg gatacgacgc attctggaag atataaaggg
     3481 tataaaagtc gtcggtgagg catcgtgcgg tgaagacgcc gttaagtggt gccggacaaa
     3541 tgccgttgac gt
//
LOCUS       DAMZET010000289         3505 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.289, whole genome shotgun sequence.
ACCESSION   DAMZET010000289 DAMZET010000000
VERSION     DAMZET010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.289"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..601)
                     /gene="ydfG"
                     /locus_tag="POB34_004697"
     CDS             complement(<1..601)
                     /gene="ydfG"
                     /locus_tag="POB34_004697"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HDI5921520.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKT"
     gene            738..2783
                     /gene="dcp"
                     /locus_tag="POB34_004698"
     CDS             738..2783
                     /gene="dcp"
                     /locus_tag="POB34_004698"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HDI5921521.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAVAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(2828..3346)
                     /locus_tag="POB34_004699"
     CDS             complement(2828..3346)
                     /locus_tag="POB34_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HDI5921522.1"
                     /translation="MNINSDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT          878 a          861 c          858 g          908 t
ORIGIN      
        1 aggttttttc cgctttaccg tcatcgcctt taaagcggac attggaaaac tcggtgccac
       61 ccaccagacc cggttcgatg tcggtgacgc gcaccgccgt accatgcaga tccgtacgca
      121 gattcaggct aaactgacga acaaacgctt tcgtcgcacc gtaaacgtta ccaccggcat
      181 acggccagct acctgccgtt gagccaatgt taataatatg accatgatta cgttcaacca
      241 tacccggtaa gacggcgcgc gtcatatata ccaggccttt gttgttggta tcaatcatcg
      301 tttcccagtc ttcaacgctg gctttatgcg caggctccat gcccaacgcc aagccggcat
      361 tatttaccag gatatcaata ttgcaccact cggcaggaag cgatgccagc atctcttcaa
      421 tagcggcgcg gttgcgaacg tccagttggg cgatatacag attatctccc agttcgtctt
      481 ttaactcctg caaccgctcc tggcgacggc cagtggcgat aactttatgc ccttgttgaa
      541 taaaacgacg agtaatgcat tcaccaaaac ctgccgttgc tccagttact aaaacgatca
      601 tctcactgtt cctcaacgct tttgtgtgtc aataacatag cacgtgatgc ttaagagcgg
      661 gtaatactgg tttgcaagat tgcactccgt tatcagtaag cctacgctat ggaacacctc
      721 gcgttcagga gaagaaaatg acaacaatga atcctttcct tgtgcaaagc acactgccgt
      781 atctggctcc tcattttgat caaattgcca atcatcacta tcgcccggca ttcgatgagg
      841 gaatgcagca aaagcgggca gaaattgctg ccatcgcgct taacccgcaa acgcctgatt
      901 tcaacaatac tattctggca ctggaacaaa gcggagaatt acttacccgc gttaccagcg
      961 tcttttttgc gatgactgcg gcgcatacca atgatgaatt acagcgtctt gatgaacagt
     1021 tttccgctga actggcggaa ctggctaatg atatctatct gaacggtgaa ttattcgcgc
     1081 gggtagatgc tgtctggcag cgccgtgaat ccctggggct tgatagtgaa tccatccgcc
     1141 tggtggaggt tattcatcaa cgttttgtcc ttgccggagc caaacttgcg caagctgata
     1201 aagcaaaatt aaaagtactg aatacagaag ctgcgaccct gaccagccag tttaaccagc
     1261 ggttacttgc agcaaataaa tccggcggtc tggttgtgaa cgatatcgcg cagctggcag
     1321 gaatgagtga gcaagagatt gcgctggcgg cagtggcggc tcgcgagaaa ggtctggata
     1381 acaaatggct gattccgctg ctgaatacca cccaacaacc ggcgcttgcc gaaatgcgcg
     1441 atcgtgcgac gcgtgaaaaa ctgtttattg cgggctggac gcgagcggaa aaaaatgatg
     1501 gcaatgatac ccgcgctatc attcaacgtc tggtagagat tcgcgcacag caggcgaaac
     1561 tgcttggttt tcctcattat gccgcatgga aaatcgccga tcagatggca aaaacacctg
     1621 aagcagcact caactttatg cgggaaattg ttccagcggc gcgtcaacgt gctagcgatg
     1681 aattagcctc catacaggcg gttatcgata agcagcaagg cgggtttagc gcgcagccgt
     1741 gggactgggc attttatgcc gaacaggtac ggcgggagaa atttgatctt gatgaggcgc
     1801 agctcaagcc atattttgaa ttaaacacgg tgttgaatga aggtgtattc tggaccgcga
     1861 atcagctctt cggtattaag tttgtcgaac gttttgatat tcctgtctac catcctgacg
     1921 ttcgtgtgtg ggaaattttt gatcataatg gcgtgggact ggcgttattt tacggtgatt
     1981 tcttcgcccg tgattcaaaa agcggcggtg catggatggg caattttgtt gagcaatcaa
     2041 cgcttaatga aacgcatccg gtaatttata acgtttgcaa ttatcagaaa cccgctgccg
     2101 gtgagcctgc gttgttactc tgggatgatg tcataacctt attccatgaa tttggtcata
     2161 cgctgcacgg cctttttgcc cgccagcgtt atgccacgct ttccggcacc aacacgccgc
     2221 gtgattttgt cgaatttccg tcgcaaatca acgaacactg ggcaacgcat ccgcaggtat
     2281 tcgctcgcta cgcccggcat tatcagagcg gggcagcaat gcctgacgaa ctgcaacaga
     2341 aaatgcgtaa tgccagcctg ttcaacaaag ggtatgagat gagcgaactg cttagcgccg
     2401 cacttctcga tatgcgctgg cattgcctgg aagaaaacga agcaatgcag gatgtcgatg
     2461 attttgaatt gcgggcgctg gtggcggaaa atatggatct tcctgctata ccgccacgct
     2521 atcgcagcag ttatttcgcc catatttttg gtggcggata tgccgcaggt tattacgctt
     2581 atctgtggac acaaatgttg gccgatgatg gttatcagtg gtttgttgag cagggcggat
     2641 taacgcgtga aaatgggctg cgttttcgcg aggcgatcct ttccagaggt aacagcgaag
     2701 atctggaacg cctgtatcga caatggcgcg gtaaggctcc tcagattatg ccgatgctgc
     2761 aacatcgtgg cttgaacata taaacaccac caatggctgt cctggagata tcgccaggcc
     2821 agcctggtta tagcagtaat tgcagcagct gggcgagggc aaacctcacc gctaaagcta
     2881 ataccgtttc gcagtcgcca gcaaaatgca taaccgcagt ataggtctgg cctttaatat
     2941 gccacgcgaa ccagacggta cccgctggcg taccatcttc accgccctcc ggtccgccgt
     3001 agccggtaat ggcaatactg acatcagcat ccgcacgctc tatggcaccg gttgccattt
     3061 ctgctgccac tttctcactc accgcagaat atcgttcaag agattgctgg cttacgctga
     3121 ggattttcat ctttgcctga tcggtgaaag taacaaagcc tgcaccgtaa aatttgggtg
     3181 tatcttcagc tgcacacagg gcgctagcca acttaccgcc ggtgcatgat tcggcggtag
     3241 ttaagcgtaa ttgatccgcg ataagtcttt gactcaacgc ggatgtcagg ttttcaatag
     3301 tgtcggtatc tgcaagttgt acaattttat cgctattaat gttcactgtt ttttgtccac
     3361 gcttttatat gtaaaggtga tttgcttttt ctgtatgctc gtccaatata ataattttca
     3421 aacatataaa tccgattatt ctgaaaatat gtttaagact cttcaatctt cttaacttta
     3481 gtgagttaaa ttctctgaat atttg
//
LOCUS       DAMZET010000290         3503 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.290, whole genome shotgun sequence.
ACCESSION   DAMZET010000290 DAMZET010000000
VERSION     DAMZET010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.290"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(85..1155)
                     /locus_tag="POB34_004700"
     CDS             complement(85..1155)
                     /locus_tag="POB34_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HDI5921523.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDKDTDKDTDQEDQN
                     TMVHGVKNATNQAGDVQTVNPGQPAGTTPEADSAYALKADSGAVQQVMTQRPEQSHQL
                     QQPEADSAIQREADRVVPENTGLSVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWK
                     ARLREGVPPEAMLDGVRRYARYLAATGKTGTEFVQRATTFFGPDRNFENPWLLPVSGT
                     NNQRCVNHISEPDNEIPPGFRG"
     gene            complement(1227..1652)
                     /locus_tag="POB34_004701"
     CDS             complement(1227..1652)
                     /locus_tag="POB34_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDI5921524.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAEITRAYFELGMTFPELHD
                     DSHPEALARNTQKIFRWVEKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(1636..1917)
                     /locus_tag="POB34_004702"
     CDS             complement(1636..1917)
                     /locus_tag="POB34_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5921525.1"
                     /translation="MEELRIFLNSLSSDEQRMFACECGTSIGYLRKALSKGQVLGASL
                     CVLIERASNGEVTRQQLRPFDWMNIWPELEDTKTLTQPLSRSLIHENQA"
     gene            2018..2437
                     /locus_tag="POB34_004703"
     CDS             2018..2437
                     /locus_tag="POB34_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921526.1"
                     /translation="MDKYEFRRQQLIKIRDEKCDGKAVNVARKIGREPSYVSRMLYPE
                     GKKGKKRIADDMVEIIEESFGLPRGWMDGIVSSSTNTASSYETRVLTPRQRIFLDLLD
                     ELPESEADNLLKTLEEKKQYYNMIYEEIRKKKAQNAS"
     gene            2703..2858
                     /locus_tag="POB34_004704"
     CDS             2703..2858
                     /locus_tag="POB34_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HDI5921527.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARRW
                     LERNSGG"
     gene            3018..3236
                     /locus_tag="POB34_004705"
     CDS             3018..3236
                     /locus_tag="POB34_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921528.1"
                     /translation="MQKREPVIIAPDYTNDELYEWMRQKINAAQDLKWANEVRAKQAE
                     NLSSLEQDITNLEKAAALTIARMITYPR"
     gene            complement(3240..>3503)
                     /locus_tag="POB34_004706"
     CDS             complement(3240..>3503)
                     /locus_tag="POB34_004706"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921529.1"
                     /translation="GGRNNPAILTAAPFRGLPQPEAHGRRKFNDRLSMNQLPRRALSR
                     YGLTFQGNILSVNCQCRMLTRVRRKHSTLPVENSLITNLSFVG"
BASE COUNT          901 a          899 c          802 g          901 t
ORIGIN      
        1 cctttaattt tttcccgctc ttcttgtgta aaaacttttg ccacaagccc tcctgaaaat
       61 tacctcatga ccagaaatta acacttaccc cctgaagccc ggcggaattt cgttatccgg
      121 ttcagaaata tgattcacac aacgctggtt gttcgtgccg cttaccggga gcaaccaggg
      181 gttctcaaaa ttccggtccg gtccaaaaaa cgtcgtcgct cgctgaacaa attccgttcc
      241 cgttttcccg gtagccgcca agtatcttgc gtaacgcctc acgccatcca gcatggcctc
      301 tggtggcacc ccctcgcgta atctggcctt ccaggcactg aaagcggatt tcttcgggtt
      361 tgctccggca cgcaacgggt actcccgcca gacctgttcg aacacatccg gataatccac
      421 tcgtcccaca gacagcccgg tgttttccgg gactacccga tcggcttccc gctgaatggc
      481 ggaatcggct tcaggctgct gcagttggtg tgattgttcc ggcctttggg tcatcacctg
      541 ctgcacagcg cccgaatcgg ctttcagcgc atacgctgaa tcggcttccg gtgtcgtgcc
      601 tgctggctga ccaggattga cggtctgaac atcccctgcc tggttcgtgg cgttttttac
      661 gccatggacc atagtgtttt gatcttcttg atctgtatct ttatctgtat ctttatctgt
      721 cgtgactcgt cgtgacatgt gcgtgacatt tcgtgatgcg ccgtgacaat cgccattttg
      781 ttcccgcttt ctttccctct ctcgctgcgc cctcttgcgc tctgcaggag attttgcggt
      841 ttgcgaaata ttgccgttgt cctctttaag cacctggcgt ttttcccatc cagtgattaa
      901 atcaccatca agtacccgcc cctgcatcgt ctgcaaaatt gaatcaatta cctcttctgt
      961 cacgtcgagc gcacttgcca aatcttctgt cgtgacatca atgtgacctc gcgtgacatt
     1021 tcgtgacgcg ctcaccagga ggtggatata cactgccatc actgttgcaa ttggctgccc
     1081 tgacaccctg gcaattgttc gccacttagg gtcatttggc atgtcatgcc ataatctgag
     1141 ccaggcgtta gccatactca cctcttctga taccgaatct ttttactcac gagttgccgg
     1201 aagcgattcg atatggctat tatcactcaa tgcactgcca cagcatttcc tgccgggcca
     1261 ccacggttca tctgattgaa accggcgatt gccactgcga caaaatcatc agcgtctctc
     1321 accagtcgct cccgcgtctc caccagctcc cgaaaataag ctgaactgtg gctgcgcatt
     1381 ctggccacca gcaaaggtgg cattgccttt tcgatcgctg gtaacaacgc ctgaattttt
     1441 tcaactgcat caggggtgtc tttctctacc cagcggaaaa ttttctgggt attgcgagcc
     1501 agggcttccg gatggctgtc gtcatgcagt tctgggaacg tcatacccag ttcaaaataa
     1561 gccctggtta tttcagctgc cggaactttt tcaccgtccg gatgcgccca ggcattcatc
     1621 gccatgcgga tgtgttcatg cttgattttc atgaatcaag ctcctagaaa gtggttgtgt
     1681 taacgttttg gtatcttcca gctcgggcca aatattcatc caatcaaaag gccttagttg
     1741 ctgacgtgta acttcaccat tactggctcg ctcaataagg acacataacg atgcccctaa
     1801 cacttgacct ttactcaatg cctttcttag ataaccgatg ctggtaccac actcgcatgc
     1861 aaacatacgc tgttcatctg acgaaagaga attgagaaat attcttaatt cttccatagc
     1921 tactccttag taaacacagc aaagaatacc tacaggtaaa caaaagtcaa tacccacagg
     1981 ttgtttacct tgcagtaatc gcatctatta tttacctatg gacaaatatg aatttagacg
     2041 acagcaactc atcaaaattc gtgatgagaa atgcgatggt aaagcggtta acgtggccag
     2101 aaagatcggg cgcgagcctt cttatgtatc aagaatgttg tacccagagg ggaaaaaggg
     2161 aaaaaaacgg atcgctgatg atatggtgga gattatcgaa gagtcctttg ggttaccccg
     2221 gggatggatg gatggtatcg tttcatcatc aacgaacaca gcctccagtt atgaaacaag
     2281 ggttctaacg ccacgacaac gtattttttt agatctctta gacgaactgc cagaaagtga
     2341 agcggataac ttattaaaaa ctcttgaaga gaaaaaacag tattacaata tgatctacga
     2401 agaaatccgt aaaaagaaag cacaaaacgc atcatagctc accaaacaac tagtcaccag
     2461 ttaagacacc gcaaaaagtt acccatgggt atttactttt taaataccta tgggtatcct
     2521 tcttttcata ccaactcacc ccgctccaca gaatgcaggg caatacttcg agttaccagg
     2581 cagtggtcag gggttaagta gccagcccga ggcgtaagaa catgacggca gggtacaact
     2641 ttaataacta tgcagcaggt ttttgttccg ctaccccggc gttaagggga aatgaggtca
     2701 gcatggatac tatcgatctt ggcaacagtg aatctctggt atgtggcgtg ttccccaacc
     2761 aggacggtac gttcaccgca atgacgtata ccaaaagcaa aacgtttaaa accgaatctg
     2821 gagcgcgtcg ctggctggaa agaaattcag gtgggtgata tggatttcga cacaatcatg
     2881 gaaaaggctt acgaagaata cttcgaaggc cttgccgaag gcgaagaagc tcacagcttc
     2941 aacgaattta aacaggcgct ttccagttcg gcaaaatcta atggctgata agcgaaacag
     3001 caccgcgagg aatcagtatg cagaaacgag aacccgtcat catcgcgcca gactatacca
     3061 atgatgaact ttatgagtgg atgcgccaga aaattaatgc agcgcaggat ctgaaatggg
     3121 ctaatgaagt cagggctaag caggctgaaa atctgtcctc tctggagcag gatatcacca
     3181 atctggaaaa agcagcggca ttaaccattg ccagaatgat tacatacccg cgttaatagc
     3241 taaccaacga agctaaggtt ggtaattaag gagttctcca cgggtaaggt ggagtgcttg
     3301 cgccggacac gggtgagcat ccggcactga cagtttactg aaaggatatt tccctgaaaa
     3361 gtcagaccat aacgcgaaag cgcacggcga ggtagctggt tcatagatag cctgtcgtta
     3421 aattttcgtc gaccgtgcgc ttccggttgt ggcaatccgc gaaatggcgc ggcggtaagt
     3481 atggcggggt tattccttcc ccc
//
LOCUS       DAMZET010000291         3482 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.291, whole genome shotgun sequence.
ACCESSION   DAMZET010000291 DAMZET010000000
VERSION     DAMZET010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.291"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..875)
                     /gene="tdcB"
                     /locus_tag="POB34_004707"
     CDS             complement(<1..875)
                     /gene="tdcB"
                     /locus_tag="POB34_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HDI5921530.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAA"
     gene            complement(974..1912)
                     /gene="tdcA"
                     /locus_tag="POB34_004708"
     CDS             complement(974..1912)
                     /gene="tdcA"
                     /locus_tag="POB34_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HDI5921531.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            2101..2445
                     /gene="tdcR"
                     /locus_tag="POB34_004709"
     CDS             2101..2445
                     /gene="tdcR"
                     /locus_tag="POB34_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HDI5921532.1"
                     /translation="MTGITIFYGDNIIRYVVNIKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            2701..3240
                     /locus_tag="POB34_004710"
     CDS             2701..3240
                     /locus_tag="POB34_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921533.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPPAHFNKTSIT
                     SDCWQILFFSPDHFNNDFY"
     gene            3262..>3482
                     /locus_tag="POB34_004711"
     CDS             3262..>3482
                     /locus_tag="POB34_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921534.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIVNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPGGKCIITGDFQKELA"
BASE COUNT         1085 a          750 c          641 g         1006 t
ORIGIN      
        1 aatgcagcac atgccagagc gcctgcgcct tcggtgacga ctttattgcg ctgaattaag
       61 gcaatcatac tgtttctgat ttcgtcttcg ctgaccagca cgatgtcatc gactaattca
      121 cgaacgattt cgtaagttaa attacccggg cgggagacat cacaaccatc cgccagggtg
      181 ccggtagttc ggtgcgtggt tatttctccg gagtggaaag aagccgccat gccgtgaacg
      241 ttttcagact gtacgccaat aacacgaatg gtcgggttaa tagatttaat tgccaccgca
      301 ataccagcaa ttaaaccgcc accaccaatt ggcacaatca cgttatcgac atcatagaga
      361 tcttccataa tttccagacc aatcgttccc tggccagcaa tcactttcgg atcatcgtaa
      421 ggtgggataa aaatacggcc ttccatttcg acaatttcgc tcactttagc gatagtgtcg
      481 ttgaaattat caccatgcag aacgacttct gcggagtagt cgcacgttgc cgctactttg
      541 gattttggcg caccttttgg catcaccact ttaccgtcga tacccagcat cgcgcaggag
      601 agcgagaccc cttgcgcatg gttgcccgca gaacaggcca ctacaccttt acgtttttcc
      661 gcatcagtca gtgaacttaa tttattaaat gcgccacgaa ttttaaatga acccgtacgc
      721 tgcatatttt caaacttcag gaatatttca cctttgcaac gttcactaaa atagtttgag
      781 cgaggcatgc ctgttttata aattcgccca gccagtcgtt gtttcgcttc aataatgtca
      841 tcaatagcaa ccggcagatc gtatgtaata tgcattataa aacctcttcg cctgaattaa
      901 atatgtaggt aaccgtaacc gacacctgca aaacagacag gtggattatt tatgtttaaa
      961 taaaaacaaa taactaacca acttctatta attgccttcg tctacaccca ttataagatg
     1021 aatactcttt ggctaattcc accaaaaccg atgctgcttt tttaatacga taatttttcg
     1081 accataccgc ggcatattgt gccacaggta atgtttcttc aaccggaata gtaataaatt
     1141 gatttgaacc aaaaggtgac gtcatatcac aaggaattac agttaagaaa tcagcattga
     1201 gaacaagatt ataaattgtc acgactgagt cggttttaac gatgttttca atactgatgc
     1261 catttctttg taacgtagta agaagctcgc tgtagtaccc catattggtt tgtggcaaca
     1321 cccactgttc gttcttcaac gactccagcg tggtggtgcc ggtgcatgtt cgggacttac
     1381 tggctaccag cacaaactcg gactcgaaca gcggctcaac atgtaaatcc tgaagcttca
     1441 tttctgcact taacgtacca atcgcaaaat ccagtcgacc gtcgcggatt gccggtaaga
     1501 acgaagacag ttgcgcttca tacatagaaa cctgcgcttt cgggaacacc tctttgaact
     1561 tgttgatcat ccctgacata aaagtaaaac caatcaatga aggaaaacca aatgagactt
     1621 ccaccaccgc ctcagaagac ataccgctta tctcattaac catatttttc atttcacggg
     1681 taatggattc ggaacgggag agtaacaatt gaccggcagg tgttaatgtt acaccggtat
     1741 ttttccgcac cactaattcc acaccaaaat aatcttcaat atcgttaatg attttactga
     1801 cggccggttg agttaaccct aattcttttg cagccgagcc gatagagcca cttctaatga
     1861 cttcctgaaa gactaccagg tgctgcgttt tcggaagaag aatagtgctc ataacgacct
     1921 acgttaatta cctcattgac ggcatgaagt gtatcaaaat gaaatgaaca ggatatgtgc
     1981 gaccactcac aaattaactt tcaatacttt ccatggtatc gttacgcaaa ttaaatacct
     2041 ttaaatatca acaagttaaa gtataaaaat cagcataaac cctgattttt gtcaaaaaaa
     2101 atgacgggga taaccatatt ttatggcgat aacatcattc gttatgtggt taatataaaa
     2161 aaggggctga gaccatattt caagcaatta ccggataatt accaggcaaa atttgagtta
     2221 aatttaatga gcaagttctc aaattttatt ataaataaac cattttcagc gataaatact
     2281 gcggcacgtc acattttttc acgctatttg ttggagaaca aacatttatt ttatcaatat
     2341 tttaaaattt ctaatacagg tattgatcat ctcgaacaat tgattaacgt caactttttc
     2401 tcttctgaca ggacgtcatt ttgtgaatgc aatcgttttc cataaattct tctcccctca
     2461 taggcgacga atagcatttt gtgttgagga tcacaaaacg aataattgct ggtcgccgcg
     2521 ataaggtcag acaaagacaa caagggaaat tttcacagag cttttgatcg tcgtaggcca
     2581 cagaatgttg cttcgtttac tgtaacgccg ggtaaatgag cgttttttga tagtgcgaaa
     2641 gaaccctgcg aggaaaataa gcatctatta ttgtttgtca tgacgacaag gcaggcatca
     2701 atgaaaggct tcccaattgc acatattttt cacccttcaa tcccgccaat gcacgcagtg
     2761 gttaacaatc acaatagaaa tattgattat tggacggtaa aaagaaagtt tgcagaaatt
     2821 gtctccacca atgacgttaa taaaatttac agtataagta atgaactgcg gagagtatta
     2881 tctgcaatta ctgcattgaa tttctatcaa ggcgatgttc cttctgtcat gatccgaatc
     2941 caaccggaaa atatgagtcc attcatcata gatatttcta caggagaaca tgacgattat
     3001 atcatacaaa cattagatgt aggcactttt gcaccttttg gtgaacaatg tacttgctca
     3061 gccgtcaata aaaaagagct ggaatgtatt aaggagacga tttctaagta ttgtgcaaaa
     3121 ttcacccgaa aagaagccat cttaacacct cctgcgcact ttaataaaac cagcattact
     3181 tcagattgtt ggcaaattct ctttttttca cccgatcatt ttaataatga tttttattga
     3241 ttatttataa aggataaaat aatgttccct gtttcatcaa ttggtaatga tattagtagt
     3301 gatttagtcc gtcgaaaaat gaatgatctt cctgaaagcc ccatagttaa taacctcgaa
     3361 gcactagcac caggtataga aaaactaaaa cagacctcta ttcaaatggt cactttactt
     3421 aacgcgttac aaccaggtgg aaaatgcatt atcactggtg attttcaaaa agaattagcg
     3481 ta
//
LOCUS       DAMZET010000292         3434 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.292, whole genome shotgun sequence.
ACCESSION   DAMZET010000292 DAMZET010000000
VERSION     DAMZET010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.292"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..354)
                     /locus_tag="POB34_004712"
     CDS             complement(<1..354)
                     /locus_tag="POB34_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDI5921535.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNV"
     gene            complement(354..1211)
                     /locus_tag="POB34_004713"
     CDS             complement(354..1211)
                     /locus_tag="POB34_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDI5921536.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(1214..1450)
                     /locus_tag="POB34_004714"
     CDS             complement(1214..1450)
                     /locus_tag="POB34_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HDI5921537.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            1651..2085
                     /gene="slyA"
                     /locus_tag="POB34_004715"
     CDS             1651..2085
                     /gene="slyA"
                     /locus_tag="POB34_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HDI5921538.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(2132..2599)
                     /gene="slyB"
                     /locus_tag="POB34_004716"
     CDS             complement(2132..2599)
                     /gene="slyB"
                     /locus_tag="POB34_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HDI5921539.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            2873..>3434
                     /locus_tag="POB34_004717"
     CDS             2873..>3434
                     /locus_tag="POB34_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDI5921540.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYD"
BASE COUNT          951 a          844 c          832 g          807 t
ORIGIN      
        1 gacgttattc gtgaagtgcg cacaggccca ggtacaaaag ccaagccacg ccgacatgct
       61 caatagaaaa aaccacggct cattgagcgt atgcccggca agaagcagtg ccgcaatggc
      121 tccgagcaaa ctgccagcga tgcgtccgag gcttttgctg ataacaccgc caacggtggg
      181 aaagctaacc actgcagccg aggtcatcgc ccaatagggt tcatccagat ttaaataata
      241 ggcaaccgtc agcgccagac acatggcaat ggtattgcgt aacgcataac gccattgcgc
      301 cagcgtggcc ctgaaccagg gcaaattgcg caaggaccat gacgatgcgt tcattattga
      361 cctacggcaa tgctacaggt ggtgcccgac accaacgtga catcgcctgg gactttatcc
      421 agcgcaaagc ggacgggaac gcgttgggcg agtcgaaccc aggggacgtt aggtttaaca
      481 tccggaataa ggcttgagtc actttcaacg ctctgatcat aaatcgcccg accgatactg
      541 gaaacgtgac cctgtaacgt tttgttatca ctatagaggg taatttgtgc tggtgcacct
      601 tcgcggatat ggcgcaactt ggtttcttca aaataaccaa tgacgtaaaa cgagtggctg
      661 tcgacaaggg caaacagcgg tttcccggtg tcagcgtaat cgccgatgcg ggtagtgagg
      721 ttagtcaccc aaccactcac cggggcgcga atttccgttt gcgccagttg ccattgtgtc
      781 tgtttcagcg tggcctgtgc ggcattaaca ctggcctgca tcgctttaac attgaggtta
      841 gcggtatcga gttcttcggc agaaataaaa ttttgtgaga gatgacgacg acgattcgct
      901 tcgttattgg cttttgcgag gtcagattgc gctttcgcca gctgagcctg tgcgttcagc
      961 tcggcgatct gaaagggcgt tttatcgatc gtgagcagaa gatcccctgc gttaaccagc
     1021 tggttgtctt ttatattcag ctcaacaata cgtcccgaca cctgtggggt gatagaaacc
     1081 tgttctgcgc gtattttccc gtcgcgtgtc cacggagatt gcatgtaata attccacagc
     1141 caccatccgg caagaaccgc aactaccgct acaatgattg ttgagaaata cttaattgtt
     1201 ttaattgaca tagttaccac gcaatcagta ttgccagagc aaggcaaacg caaatcgcaa
     1261 acagcgataa atccattaac aagggatgcc agatgtcacc ggcgtagatc cagccacgaa
     1321 gcaggcggtg tacgacaagc cagatgacga atccaaacgc gaaggctttg aaaaacggag
     1381 gaaagtagac ggacgcaccg aacaccagat cttgtaaggg caatcctgtt gcattgagca
     1441 taaacttcac aaagtagatt cctttacgac cgaatatatt gcgttagatt aataaatatt
     1501 ctttaagtgc gaaaaattta cgcgcaattt ctgaaaaata tgtctgacgg taaccaaatg
     1561 cagcaataca tttgttttag caatacaatt gctgcacact attctaaaag ccgcataata
     1621 tcttagcaag ctaattataa ggagatgaaa ttggaatcgc cactaggttc tgatctggca
     1681 cggttggtgc gcatatggcg tgctctgata gaccatcgcc tgaaaccgct ggagttaaca
     1741 caaacccatt gggttacgtt acacaatatc catcagttac ctccagacca gtcgcaaatt
     1801 caactggcaa aagcgattgg catcgagcag ccatcactgg tccgtactct ggaccaactg
     1861 gaagaaaaag ggttaatttc gcgtcaaact tgtgccagcg atcgtcgggc taaacgtatt
     1921 aaactgacgg aaaaggcaga gccactgatc agcgaaatgg aagctgttat taacaaaacc
     1981 cgcgcggaaa tattacatgg catctccgca gaggaactgg aacaactgat tacgctcatc
     2041 gcaaaacttg agcataatat cattgagtta caggccaaag ggtgaaatga agggggcgtg
     2101 tggtcagtaa cctgaccaca cgcaaactta tttagcgcgg agaaacggtc acctgactgc
     2161 cattgctggc cagtacgaca cgttggcccg gagagaaacg agtgttgcct tgtttctgta
     2221 ccaccatgat ggtattacca tcgtctttac gaatttccag ctcgacaccc tgcgttttgt
     2281 tcattgcact ctgtacgccc tgaccagcta cgccacctgc aacagcgcct gctgcagtag
     2341 ccagagaacg cccggttccg ccaccaacag tattccccag gaaaccacca agaacagcac
     2401 cgccaattgc accgataacg ttggaatcat caccgccctg aatctgtacc ggacgtacat
     2461 taacgatggt gccatagctg acattctgta cttgtttcgc ttcagaagcg gtataaacat
     2521 cccctgacag ggtgtcgtta ttaacacaac cgacaagaga cagacctacc attgaaacaa
     2581 ccaatacgcg tttaatcatt gaaaaatctc ctgttcacca tgaaacgcca cgcaagcatc
     2641 cctcatggtc aaagtatatg gcatatgtga atccatggtt acatgttcta ccaaatatga
     2701 gcaaatcata atgaaagtag gatcaaccaa ttataaacaa aacattcatg atgattattg
     2761 atttgaagat aatgttttac taccaacggt aaaaatcggt cattccgcat ggtattcacg
     2821 aaaagtttat gtttgagtgg tgcgcaaacg tcagattgaa aaaggatgaa ctatgaaatc
     2881 gggccgcttt attggcgtta tgtcaggcac cagccttgat ggtgttgatg ttgtgttggc
     2941 gacaatagat gaacaccggg tcgcacagct ggcaagcttg agctggccga tcccggtgtc
     3001 tctgaaacag gctgtactgg atatttgcca gggccagcag cttacacttt cgcagtttgg
     3061 acagcttgat actcaactcg ggcgactttt tgctgatgcg gtcaatgcct tgcttaagga
     3121 acaaaacctg caggcgagag atatagttgc gatcggctgt cacggtcaaa ccgtctggca
     3181 tgaaccgacg ggcgtggcac cacacacttt acagattggc gataacaatc aaattgtggc
     3241 acgcaccgga attacggttg tcggtgattt tcgccgtcgc gatattgcct tgggaggaca
     3301 aggcgcaccg ctggtacctg cgttccatca tgccttgctg gctcacccaa ccgagcgacg
     3361 aatggtgctc aatattggcg gcatcgccaa tctgtcactg ctcattcctg ggcagccggt
     3421 tggcggctac gata
//
LOCUS       DAMZET010000293         3410 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.293, whole genome shotgun sequence.
ACCESSION   DAMZET010000293 DAMZET010000000
VERSION     DAMZET010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.293"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..674
                     /gene="ydfJ"
                     /locus_tag="POB34_004718"
     CDS             <1..674
                     /gene="ydfJ"
                     /locus_tag="POB34_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HDI5921541.1"
                     /translation="PAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQ
                     PSRSL"
     gene            763..2223
                     /locus_tag="POB34_004719"
     CDS             763..2223
                     /locus_tag="POB34_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDI5921542.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2259..2462)
                     /gene="ydfZ"
                     /locus_tag="POB34_004720"
     CDS             complement(2259..2462)
                     /gene="ydfZ"
                     /locus_tag="POB34_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HDI5921543.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2640..3326)
                     /gene="ydfH"
                     /locus_tag="POB34_004721"
     CDS             complement(2640..3326)
                     /gene="ydfH"
                     /locus_tag="POB34_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HDI5921544.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
BASE COUNT          842 a          767 c          880 g          921 t
ORIGIN      
        1 cacctgctgg tagcatgttc cagagcaaat ccttctggct ggcaacaggg ctgcgttttg
       61 gtcaggcggg taactccggg ttaattcaga ctttccttgc aggctattta gtgcagacgt
      121 tattgtttaa caaagcaatt ccaacagatg cattgatgat tagttcgatt ctcggcttta
      181 tgaccattcc gttccttggt tggttatccg ataaaattgg tcgccggatc ccgtatatta
      241 ttatgaatac ctccgcgatt gtgctggcat ggccaatgct ttctatcatt gtagataaaa
      301 gctatgcccc gagcaccatt atggttgcac tgattgtgat tcataactgt gcggtgctgg
      361 gattatttgc tctggaaaac attaccatgg cagaaatgtt cggctgtaaa aaccgcttta
      421 cccggatggc tatttctaaa gaaattggtg gtcttatcgc ttccggtttt ggtcctatcc
      481 tggcgggtat tttctgcacc atgacggaat cctggtatcc gatcgccatt atgatcatgg
      541 catattcagt aattggttta atctctgcgc tgaaaatgcc agaggtgaaa gaccgtgatt
      601 taagtgcgct ggaagacgct gcggaagatc aaccgcgtgt tgtaagagct gcgcaacctt
      661 ccagaagtct gtaaatcctc aatcccttct cttaccggag aggggatttt tattcatata
      721 acaaaacata tagcttgcca tatttatatt taaggaattg ttatgggaaa taatttgtta
      781 tcagcaaaag cgacactccc tgtttatgat cgtaataacc tggccccaag aattattcat
      841 ttaggctttg gtgcatttca ccgtgcgcat cagggcgtgt atgccgatat tcttgctaca
      901 gaacatttca gtgactgggg atattatgag gtcaacttaa tcggcggcga acagcaaatt
      961 gccgatttac agcagcaaga taatctttat accgttgcgg aaatgtcggc cgatgcgtgg
     1021 acggctcgcg tcgttggcgt cgttaaaaaa gccttgcacg tacagatgga tggcttagaa
     1081 accgtgttgg ctgcgatgtg tgaaccgcaa atcgcgattg tctctctgac aatcaccgaa
     1141 aaagggtatt tccactctcc ggcgaccgga cagttaatgc tcgatcaccc gatggtcgct
     1201 gccgacgtgc aaaatcccca ccagccgaaa acagcaacag gggtgattgt tgaggcgctg
     1261 gcccgccgta aagcggcagg acttcccgca tttaccgtca tgtcatgtga caacatgcca
     1321 gaaaacggtc atgttatgcg tgacgttgtc acttcatacg cgaaagctgt tgatgtaaaa
     1381 ctggcacaat ggatcgaaga taacgtgact ttcccatcaa caatggtgga ccgtattgtg
     1441 cccgcagtga cagaggatac gctggcgaaa atcgaacaac ttaccggtgt gggcgatcct
     1501 gcgggcgttg cctgtgaacc tttccgccag tgggtaatag aagataactt tgttgccgga
     1561 cgtccagaat gggaaaaagc gggagccgaa ctggttagtg atgtgctgcc ttatgaagag
     1621 atgaagttgc gcatgctcaa cggcagtcat tcattcctgg cgtatttggg ttatcttgct
     1681 ggatatcagc acattaatga ctgcatggaa gatgaacatt atcgttatgc ggcgtatggc
     1741 ttgatgttgc aggaacaagc gccgacgctg aaagtgcagg gcgttgattt gcaagattac
     1801 gctaaccgat taattgcacg ctatagcaac ccggcgttac gtcatcgaac ctggcagatt
     1861 gcgatggatg gtagccagaa attgccacag cggatgttgg attctgttcg ctggcatctg
     1921 gcgcatgaca gcaagttcga tctgctggcg ctgggcgtcg cgggttggat gcgttatgtc
     1981 ggtggtgttg atgaacaggg aaatccgata gaaatcagtg acccactgtt acctgttatt
     2041 cagaaggctg tacaaagtag tgccgaaggg aaagcgcgcg tccagtcatt gctggcgatt
     2101 aaggcaatct ttggtgatga tttgccagac aatagcttgt ttactgcaaa agtgacggaa
     2161 gcgtacttgt ctttattagc gcatggcgcg aaagcgaccg tggcgaaata ttccgtgaag
     2221 taaaaaaagc aatgccgcca tcggcggcat tcacacactt agttcatgcc gagacgaatc
     2281 aggtccagag gtgccagttt ctcttcacaa ccttcaacaa ctacggtttt tccgcggcgg
     2341 atttgctcag cggtcagacc ttcagtatca atcgacagga tcttgccagt gtgaccggtg
     2401 ccgctaacca taacacggct gccagtggtg attgcgttac ggttacgatc gtaggtcgtc
     2461 ataggtgttt tctcctttct gatttacagt cgaagcccac gtaacgggcg caatataaat
     2521 actcctgtcg agtgaaatat tttttgtttt tgatcaagct cacatctttt ttgatgcatg
     2581 catgagccgt aaggcggggg gaaaagcctc tcatcccgga tgggatgaga gtaaatgaat
     2641 tactcttcgc taaaccagtc gctgttttcc tggcggatct ggcgcacgga ttcgctgatt
     2701 tcctgcaaat gttgcgtcat tgcacgttct accgcatcgc catcacgttt ttgcagggca
     2761 gagaaaatat caagatgctg gcgtaacagc atttctggtg gagaaacatg gtcgaaactc
     2821 atatagcgca cgcgatcaac ggtcgctttc aggttctcaa tggtatccca cgccagttga
     2881 cagtcggcaa tctgcgtcag gagttgatgg aagttgtcat caagttcaaa aaaatcatcc
     2941 agttgcttgc gctcaatggc aatgcgttgc tggtgaagat tttgttccag ttggtagcac
     3001 tggctttcgg taatcatgct cgccgcccga cgcgccaccg cgcactcaat ggcctgacgg
     3061 ataaaactgc cgtttcgcac ctgggccatg gaaattttgt tgacgtagct gccacgctgc
     3121 ggacgaattt gaatcaggcc gttttccgcc agtttaataa aggcttcacg aaccggctgg
     3181 cgtgacacat tgaaacgaac agaaacttct ttttccgaca acggtgtgcc tggtgcaatc
     3241 aggcaatgga caatgtcgcg acgaagaata cgataaatct gctgattgac aggctgtgtg
     3301 ggattaagtt gcgtttcgac ggtcattcgt tcttgcttgt gagtgagtta actgcatgag
     3361 tctaccactt ttttgtgaca agcaataacc cggcagttac gccgggtata
//
LOCUS       DAMZET010000294         3366 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.294, whole genome shotgun sequence.
ACCESSION   DAMZET010000294 DAMZET010000000
VERSION     DAMZET010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.294"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            66..1763
                     /gene="maeA"
                     /locus_tag="POB34_004722"
     CDS             66..1763
                     /gene="maeA"
                     /locus_tag="POB34_004722"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDI5921545.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1897..2907
                     /gene="adhP"
                     /locus_tag="POB34_004723"
     CDS             1897..2907
                     /gene="adhP"
                     /locus_tag="POB34_004723"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HDI5921546.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVCTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3053..3337
                     /locus_tag="POB34_004724"
     CDS             3053..3337
                     /locus_tag="POB34_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDI5921547.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
BASE COUNT          866 a          810 c          920 g          770 t
ORIGIN      
        1 cctggcggta aagcaaagac gataaaagcg tgccagggat ggatattcaa aaaagagtga
       61 gtgacatgga accaaaaaca aaaaaacagc gttcgcttta tatcccttac gctggccctg
      121 tactgctgga atttccgttg ttgaataaag gcagtgcctt cagcatggaa gaacgccgta
      181 acttcaacct gctggggtta ctgccggaag tggtcgaaac catcgaagaa caagcggaac
      241 gagcatggat ccagtatcag ggattcaaaa ccgaaatcga caaacacatc tacctgcgta
      301 acatccagga caccaacgaa accctcttct accgtctggt aaacaatcat cttgatgaga
      361 tgatgcctgt tatttatacc ccaaccgtcg gcgcagcctg tgagcgtttt tctgagatct
      421 accgccgttc acgcggcgtg tttatctctt accagaaccg gcacaatatg gacgatattc
      481 tgcaaaacgt gccgaaccat aatattaaag tgattgtggt gactgacggt gaacgtattc
      541 tggggcttgg tgaccagggc atcggcggga tgggcattcc gatcggtaaa ctgtcgctct
      601 ataccgcctg tggcggcatc agcccggcgt atacccttcc ggtggtgctg gatgtcggaa
      661 cgaacaacca acagctgctt aacgatccgc tgtatatggg ctggcgtaat ccgcgtatca
      721 ctgacgatga atactatgaa ttcgttgatg aatttatcca ggctgtgaaa caacgctggc
      781 cggacgtgct gttgcagttt gaagactttg ctcaaaaaaa tgcgatgccg ttacttaacc
      841 gctatcgcaa tgaaatttgt tcttttaacg atgacattca gggcactgcg gcagtaacag
      901 tcggcacact gatcgcagcc agccgcgcgg caggtggtca gttaagcgag aaaaaaatcg
      961 tcttccttgg cgcaggttca gcgggatgcg gcattgccga aatgatcatc gcccagaccc
     1021 agcgcgaagg attaagcgag gaagcagcgc ggcagaaagt ctttatggtc gatcgctttg
     1081 gcctgctgac ggacaagatg ccgaacctgc tacctttcca gaccaaactg gtgcagaagc
     1141 gcgaaaacct cagtgactgg gataccgaca gcgatgtgtt gtcactgctg gatgtggtgc
     1201 gcaatgtaaa accagatatt ctgattggcg tctcaggaca gaccgggctg tttacggaag
     1261 agatcattcg tgagatgcat aaacactgtc cgcgtccgat cgtgatgccg ctgtctaacc
     1321 cgacgtcacg cgtggaagcc acaccgcagg acattatcgc ctggaccgaa ggtaacgcgc
     1381 tggtcgccac tggcagcccg tttaatccag tggtatggaa agataaaatc taccctatcg
     1441 cccagtgtaa caacgccttt attttcccgg gcatcggcct gggggttatt gcttccggcg
     1501 cgtcacgtat caccgatgag atgctgatgt cggcaagtga aacgcttgct cagtattcgc
     1561 cgctggtcct gaacggcgaa ggtctggtac taccggaact gaaagatatt cagaaagtct
     1621 cccgcgcaat tgcgtttgcg gttggcaaaa tggcgcagca gcaaggcgtg gcggtgaaaa
     1681 catctgccga agctctgcaa caggccattg atgataattt ctggcaagcc gaataccgcg
     1741 actaccgccg tacctccatc taagcctgcg cccggtagtg aaggctaccg ggctatttct
     1801 ctctcccttt ttcagatctc atccatactg ggtagtggcg aataaatctc atttgcctca
     1861 cctgctatgc agaacatcat ccgaaaagga ggaactatga aggctgcagt tgttacgaag
     1921 gatcatcacg ttgacgttac ggataaaaca ctgcgctcac tgaaacatgg cgaagccctg
     1981 ctgaaaatgg agtgttgtgg tgtatgtcat accgatcttc atgttaagaa tggcgatttt
     2041 ggtgacaaaa ctggcgtaat tctgggccat gaaggcatcg gtgtggtggc agaagttggc
     2101 ccaggtgtca cctcattaaa accaggcgat cgtgccagcg tggcgtggtt ctacgaagga
     2161 tgcggtcatt gtgaatactg taatagtggt aacgaaacgc tctgccgttc agttaaaaat
     2221 gccggataca gcgttgatgg cgggatggcg gaagagtgca tcgtggtcgc cgattacgcg
     2281 gtaaaagtgc cagatggtct ggactcggcg gcggccagca gcattacctg tgcgggggtc
     2341 accacctaca aagccgttaa actgtcaaaa attcgtccag ggcagtggat tgctatctac
     2401 ggtcttggcg gtctgggtaa cctcgctctg caatacgcga agaatgtctt taacgcgaaa
     2461 gtgatcgcca ttgatgtcaa tgatgagcag ttaaaactgg caaccgaaat gggtgcagat
     2521 ttagcgatta actcacgcac cgaagacgcc gccaaaattg tgcaggagaa aaccggtggc
     2581 gctcacgctg cggtggtaac agcagttgct aaagctgcgt ttaactcggc agttgatgct
     2641 gtccgtgcag gcggtcgtgt tgtggctgtc ggtctgccgc cggagtctat gagcctggat
     2701 atcccacggc tggtgctgga tggcattgaa gtggtcggtt cgctggtctg cacgcgccag
     2761 gatttaactg aagccttcca gtttgccgcc gaaggtaaag tggtgccgaa agtcgccctg
     2821 cgtccgttag cggacatcaa caccatcttt actgagatgg aagaaggcaa aatccgtggc
     2881 cgcatggtga ttgatttccg tcactaagag gcctttgctg cgactgccat gttcgggtcg
     2941 cagcatcgca cactctccaa catgaaatgg ctaaatggat tattgttgcg taggaaggaa
     3001 caataatgat agtgttccca gtgaaggaac gctactgaga gtaaagacac taatgaaaat
     3061 ggccaatcat ccccgcccgg gggacattat tcaggaatca ctggacgaac ttaatgtcag
     3121 cctgcgcgag tttgccagag caatggaaat tgctccctca acggcaagcc gattgctgac
     3181 aggaaaagca gctttgacgc cagaaatggc gattaaactt tccgtggtga tcggcagttc
     3241 gccgcaaatg tggctgaatc tgcaaaacgc ctggagtctg gcagaggcag aaaaaacagt
     3301 ggatgtgtcg cgattacgcc gtctggttac gcaataaaaa aatggcactt tataaagcgc
     3361 cattta
//
LOCUS       DAMZET010000295         3284 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.295, whole genome shotgun sequence.
ACCESSION   DAMZET010000295 DAMZET010000000
VERSION     DAMZET010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.295"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..211
                     /gene="nadE"
                     /locus_tag="POB34_004725"
     CDS             <1..211
                     /gene="nadE"
                     /locus_tag="POB34_004725"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HDI5921548.1"
                     /translation="KKAPTADLEDDRPSLPDEVALGVTYDNIDDYLEGKNVPEQVART
                     IENWYLKTEHKRRPPITVFDDFWKK"
     gene            441..1328
                     /gene="cho"
                     /locus_tag="POB34_004726"
     CDS             441..1328
                     /gene="cho"
                     /locus_tag="POB34_004726"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HDI5921549.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEDATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(1288..1863)
                     /gene="ves"
                     /locus_tag="POB34_004727"
     CDS             complement(1288..1863)
                     /gene="ves"
                     /locus_tag="POB34_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HDI5921550.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(2066..2551)
                     /gene="spy"
                     /locus_tag="POB34_004728"
     CDS             complement(2066..2551)
                     /gene="spy"
                     /locus_tag="POB34_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HDI5921551.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(2881..>3284)
                     /locus_tag="POB34_004729"
     CDS             complement(2881..>3284)
                     /locus_tag="POB34_004729"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HDI5921552.1"
                     /translation="PGGTFTHFSARHFGALACTLELGKALPFGQNDLRQFAVTASAIA
                     ALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFMPFEKGTLLAQDGEER
                     FTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT          829 a          797 c          802 g          856 t
ORIGIN      
        1 taagaaagcc ccaacggccg atctggaaga tgatcgccct tctctgccag atgaagtggc
       61 actcggcgtg acctatgaca atatcgacga ctatctggaa gggaaaaacg tacctgaaca
      121 ggtcgccaga acaatagaga actggtatct gaaaaccgaa cataaacgcc gtccgccaat
      181 taccgttttc gatgatttct ggaaaaagta ataatttgca cataatggtt tgtgcgaaaa
      241 agagtaattt gttcacgccg gatgcggcgt gaacgcctta ttcgacctac aaaactatgc
      301 aaattcaata tattgcagga gtcgaatagg cctgacaggc gtagcacgtc agacggtgta
      361 acctttgtca tcgacccgcc tcttttttaa tcgctccccg cctgttacac tggatagata
      421 accagcattc ggagtcaaca gtggtacggc gtttaacttc tccgcggctc gaatttgaag
      481 ctgcggcaat ttatgaatat cccgaacatt tacgttcatt ccttaatgac ttacccaccc
      541 gaccaggggt gtatctgttt catggcgaaa gtgacaccat gccgctctat atcggcaaaa
      601 gcgttaatat ccgcagtcgg gtgctttctc atttacgcac cccggatgaa gccgccatgc
      661 tacggcaatc ccggcgaatt agctggatct gcaccgcagg cgaaatcggc gctctgctcc
      721 ttgaagcgcg attaatcaaa gaacaacagc cgctgtttaa taaacggttg cgccgcaatc
      781 gccagctctg tgccctacaa ttaaatgaaa agcgcgtcga tgtggtgtat gccaaagagg
      841 tggatttttc acgagccccc aacctgtttg gcctgtttgc caataggcgc gcagctttgc
      901 aagcattgca gagcatcgct gatgaacaaa aactttgtta tggcctgctg ggactggaac
      961 cgttaagtcg cggtcgtgca tgttttcgtt cagcgctaaa acgttgcgcc ggagcatgct
     1021 gcggtaaaga gagccatgag gaacatgcgc tacgcttgcg ccagtctctg gagcgtttgc
     1081 gggtggtgtg ttggccttgg caaggggcgg tggcgctgaa agaacagcac ccggaaatga
     1141 ctcaatatca tattattcaa aactggctgt ggctgggggc ggttaattcg ctggaagacg
     1201 cgacaacgtt aattcggaca cccgccgggt ttgatcacga cggttataaa attctttgta
     1261 agccgctgct ttccggtaac tatgaaatta ctgaacttga tccggcgaat gaccagcgag
     1321 ccagttgatt tcactgaaca acagtttacc ttgcggttgt aaaagccgca gggtgtgcct
     1381 gccatcgaac cagcacgcgc cctggtcggt agtgagcaat ttatcgccca gttgccacgc
     1441 cccgttaatc acaaacacca ccccgccgcg tgagccaaag gtagtaaacg tacgttcggc
     1501 aattctgact ttcgccttac agacatccag gcgtgtcatg atgttgaaat ccatcgacat
     1561 ctgccctgcc gtcagtttcg cttttaccac ctggtccgct gcaaaggcaa aaggctgtaa
     1621 cggttttaag gtatggttaa agcggtctgc gctttcaagg agcatctcgc cgccttccag
     1681 caacgtcact atcctttcca tgccgggaaa caaagaaaac tccccattcg ccgcgatgga
     1741 tgcaatgctg gcacgccagt aaaaatcacg ttttgccggt gggaaagtgc aaatttcgcg
     1801 cgtttcaccg gcagcatttc gccacagatt caccgacatt ttacgcatat caaagtattc
     1861 catactcgct cccggcccgt tcttttcatt gttatcgttc aggtaaccgt ggatggttat
     1921 cggcaactcg ggtgatttac gttagtggtg atcaggcttt ttttacaact gtttgagaag
     1981 agaaaagaaa accgccgatc ctgtccaccg cattactgca aggtagtgga caagaccggc
     2041 ggtcttaagt tttttggctg aaagattatt cagcagttgc aggcatttta ccttttgccg
     2101 ctggacgttc tgtcagacgc ttctcaaaat tagcattaaa ttgctttttc tgttccggcg
     2161 tcaggatgtt gtaaattttg ttctgggttt ccatgtgcgc cagcatgtta gctttgcgct
     2221 gttcttccat ttttgcgatc tgcgcttcag cttttgcttt atcgaaggta tcgctggcaa
     2281 tgatgtcatg cattgcgcgg cgttcttcca gcggcggacg tttcatctgg tcacgctggc
     2341 ctttcatgat ttcgcggatc tgctgtttct gcgcgtcggt caggttcagg tctttgaaca
     2401 tcatgtcctg atgcggaccg aacttgcctt tgtggtgcat catcggcttc gcgtcagccg
     2461 gtgctgcggt agtggtgtct gcggcatggg ccaggttagc cgcgccaaga gccagggtag
     2521 aggcaacaaa cagtgcagtt aatttacgca tattctatat ccttcctttc agttatttat
     2581 tacggctttc ttaagtagcg tgccgtgttg acgagattaa ctttactgac tttagcgtca
     2641 attaatcaga gcaacggtaa aacaatgaaa gtgtaaaaaa cacttttgcg ccaattatgg
     2701 agaaaaaaag aaaatttgat ggagagtgat gagagaataa tacaacacga tgattttgca
     2761 gagattatga agaactatac cggatgactg gtgataaata aagcaaataa ccaggattaa
     2821 tctgtattaa tttataagaa agcaacttaa tacccgcaga atgatttctg cgggtaagta
     2881 ttagcttatt ttttcgagca ttaatcccgc gcgtaatccc aacgctacca acggattagg
     2941 gaataacaca tactctacat catgggttac ggtaaaacgt tcctctccgt cctgcgccag
     3001 caatgttcct ttctcaaacg gcataaaatt cagcgtgtca cttgccatat gcatttcgaa
     3061 ggacggcgag tggcgagtaa tttgcgaaac cacccgataa cggagcggcg gtgttctcac
     3121 gataccgaca ctctcaccag atagcagcgc agcaattgcg ctggcagtta ctgcaaactg
     3181 gcgaagatcg ttttgcccaa agggcaacgc tttgccaagt tccagcgtac aggccagcgc
     3241 gccaaaatgt ctggcgctga aatgggtaaa cgtaccacca ggtt
//
LOCUS       DAMZET010000296         3253 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.296, whole genome shotgun sequence.
ACCESSION   DAMZET010000296 DAMZET010000000
VERSION     DAMZET010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.296"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..613
                     /gene="eutR"
                     /locus_tag="POB34_004730"
     CDS             <1..613
                     /gene="eutR"
                     /locus_tag="POB34_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HDI5921553.1"
                     /translation="VITRQANFLHNPDRVLHMLRSQSALEVKEQHKAALWGFVQQALA
                     TFCENPENLHQPAVRKVLGDNLLMAMGAMLEDAQPMVTAESISHQSYRRLLSRAREYV
                     LENMSEPVTVLDLCNQLHVSRRTLQNAFHAILGIGPNAWLKRIRLNAVRRELISPWSQ
                     STTVKDAAMQWGFWHLGQFATDYQQLFAEKPSLTLHQRMREWG"
     gene            complement(619..1518)
                     /gene="hemF"
                     /locus_tag="POB34_004731"
     CDS             complement(619..1518)
                     /gene="hemF"
                     /locus_tag="POB34_004731"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HDI5921554.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKSYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(1522..2391)
                     /gene="amiA"
                     /locus_tag="POB34_004732"
     CDS             complement(1522..2391)
                     /gene="amiA"
                     /locus_tag="POB34_004732"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HDI5921555.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            2605..3030
                     /locus_tag="POB34_004733"
     CDS             2605..3030
                     /locus_tag="POB34_004733"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HDI5921556.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            3017..>3253
                     /locus_tag="POB34_004734"
     CDS             3017..>3253
                     /locus_tag="POB34_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 family protein"
                     /protein_id="HDI5921557.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSG"
BASE COUNT          743 a          813 c          887 g          810 t
ORIGIN      
        1 tgtcatcacc cggcaggcta actttttgca taacccggat cgggtattac atatgctgcg
       61 tagccagtcg gcgctggaag tgaaagagca gcataaagcc gcgctgtggg gctttgtcca
      121 acaggcgctg gcgacgtttt gcgagaaccc ggaaaatctc catcagcctg cagtgcgaaa
      181 agtgctgggg gataatttgc taatggcgat gggggcgatg ctggaagacg cgcaaccaat
      241 ggtgacggcg gaaagcatca gtcatcagag ttaccgtaga ttactttccc gcgcccgtga
      301 atatgtgctg gaaaatatgt ctgagccggt gacggtgctg gacttgtgta atcaactgca
      361 tgtcagtcgc cgcacgctac aaaacgcgtt tcacgctatt ttaggcattg gcccaaacgc
      421 gtggctgaaa cgcattcgcc tgaacgccgt acgccgcgaa ctgataagcc cgtggtcgca
      481 aagcacaacg gtaaaagacg ccgccatgca gtggggattc tggcatctgg ggcaatttgc
      541 cacggattac cagcagctgt ttgccgagaa gccgtcgttg acgttgcatc agcggatgcg
      601 ggaatggggg tgagggagtt acacccaatc cctgacctta ataaactcac ttaacgccgc
      661 ttctgggctg ccatcttttg gctgataatc atactcccag cgtaccagtg gcggcattga
      721 catcaggatc gactcggtgc gcccgccagt ttgcaggcca aacagagtgc cgcgatccca
      781 gaccagattg aactcgacat aacgaccgcg acggtagagc tgaaaattgc gctcgcgctc
      841 gccgtaggcc atcgctttac gtcgctctac aattggtaaa taagcgtcgg tgtagctttt
      901 gcctaccgcc tgcataaagg caaaacagtg gtcgaaatct ggcgtgttca gatcatcaaa
      961 gaacagcccg ccaataccgc gctgttcgtt gcgatgtttg aggtagaagt aatcgtcgca
     1021 ccactttttg taacggggat aaacgtcttc accaaatggc aggcacaggt cacgggcggt
     1081 gcggtgccag tgaatggcgt cttcttcaaa accatagaaa ggggttaaat cgaagccgcc
     1141 gccaaaccac cagacgggct ctgcacccgg tttttcggca ataaaaaaac gcacattcgc
     1201 gtggctggtg ggaacatacg ggttatgcgg atgcactacc agtgaaacgc ccatcgcctc
     1261 gaaactgcgc ccggcaagtt ccgggcgatg agcggtggcg gaagctggca tcgcctcgcc
     1321 gtggacgtga gaaaagttga cgcctgcctg ttcgaaaaca ccaccattac gcaacacccg
     1381 actacgcccg ccgccgccag cttcgcgctg ccaactatct tcgacaaatt ctgcgccatc
     1441 gacggcggtc agctgctgac aaatcgtatc ctgaaggttg agcagaaact gtttaacctg
     1501 gtgtgcgtcg ggtttcataa cttatcgctt tttcgaatgt gctttctggt tgtcgaacca
     1561 gtggaaataa ctgatcacgc cttcagcaat cgctgtggcg attttctgac gaaacgccgc
     1621 cgtgcctaac agccgctctt cttccgggtt ggtgataaac gaggtttcca ccagcaccga
     1681 aggaaccgac ggcgatttca acaccacaaa cgcggcttgt tcggtgttgc ggctgtgcag
     1741 tttatgcacc ggcttaatct ttttcagaat atgcgagccg agcgtcaggc tgtttttaat
     1801 agtatcggtt tgcaccagat caaacagcac ttgctgcaat aggtgatcct tgtcagtcgc
     1861 ctttttaccg gcaacttcat cggcgcggtt ttcgcgttca gacaggtatt tcgccattgc
     1921 gctacttgcc ccacggttag agagggcaaa taccgaagca ccggcagctt tcgggttggt
     1981 aaagccatcg gcatgaattg acataaacag atctgcgcca tgtttatggg cgatttcaac
     2041 gcgatcgtaa agtgggataa acgtatcgcc agaacgcgtt aaacgcgcat caatcccatg
     2101 attacgcaaa atggaacgga cgtttttagc aatcgccagc accacatgtt tttctttcga
     2161 accgttgcgt ccgatcgctc cggtatcaat tccgccgtgg cctggatcga gaacaacgac
     2221 acgtttgccg ccagattttt tggctttcgg cttgctgtgt ccgttgctgg tttttaaagg
     2281 ttcgtctttg gcgatggctt gcgacattcc tgacaacgtc agggcagcca aaccggcttt
     2341 cagcacctgg cggcgcgaag tgagtgtttt tagtggttta aaagtgctca tacggcctga
     2401 gttgtaataa taaagttcca gatgttatat cgtatcgcgt aatccgttac gacggtttga
     2461 tgttgagaat tgttttactt ttcatttcaa tagttgacaa ctccccatta tgccactttt
     2521 tcaaccgatt ttcgtcagat attggctaat tcgaccgttc acgccataat cagcctttta
     2581 acccgcaaaa aggtaacgaa taccatggag atacgcgtat ttcgccagga agatttcgaa
     2641 gaggtcatca ccctttggga gcgttgcgac ttgctgcgtc cgtggaacga tccggaaatg
     2701 gacatcgagc gtaagatgaa ccatgacgtc agtttgtttt tggtcgctga ggtaaacggt
     2761 gaagtggtcg gaacggtgat gggcggttat gacgggcatc gcgggtctgc ttattatctt
     2821 ggcgtgcatc cagagtttcg tgggcgtggg attgccaatg cgttgcttaa tcggctggag
     2881 aaaaagctga ttgctcgtgg ctgcccgaaa attcagatca atgtgccgga agataacgac
     2941 atggtgctcg gaatgtatga acgcctggga tatgaacacg ccgacgtgct gagtctgggt
     3001 aagcgtttga ttgaagatga agagtactga gttccatcct gtccattatg atgcgcacgg
     3061 acgcctgcgt ttacccttgc tcttctggct tgtgctattg cttcaggcgc gaacctgggt
     3121 gctgtttgtc attgccggtg cgtcgcgtga gcagggtact gcgctgctaa atctgtttta
     3181 tcccgatcac gataatttct ggctggggct gattcctggc attccggcgg tgctggcgtt
     3241 tttgttgagc ggt
//
LOCUS       DAMZET010000297         3194 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.297, whole genome shotgun sequence.
ACCESSION   DAMZET010000297 DAMZET010000000
VERSION     DAMZET010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.297"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..422)
                     /gene="exoX"
                     /locus_tag="POB34_004735"
     CDS             complement(<1..422)
                     /gene="exoX"
                     /locus_tag="POB34_004735"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HDI5921558.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDC"
     gene            complement(446..1102)
                     /locus_tag="POB34_004736"
     CDS             complement(446..1102)
                     /locus_tag="POB34_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDI5921559.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(1204..1434)
                     /gene="holE"
                     /locus_tag="POB34_004737"
     CDS             complement(1204..1434)
                     /gene="holE"
                     /locus_tag="POB34_004737"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HDI5921560.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            1573..1947
                     /gene="yobA"
                     /locus_tag="POB34_004738"
     CDS             1573..1947
                     /gene="yobA"
                     /locus_tag="POB34_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HDI5921561.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYI
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            1951..2823
                     /gene="copD"
                     /locus_tag="POB34_004739"
     CDS             1951..2823
                     /gene="copD"
                     /locus_tag="POB34_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HDI5921562.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            2836..3177
                     /locus_tag="POB34_004740"
     CDS             2836..3177
                     /locus_tag="POB34_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HDI5921563.1"
                     /translation="MMKKSILTFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
BASE COUNT          810 a          774 c          815 g          795 t
ORIGIN      
        1 taacagtcat acaacgcgcg gtgatgatgc aggcccggcg gcgtctgtac attgagcttg
       61 cgtgttttat ataacgccat attgctgtac ttgatcccag gccacaaacg acgggccagt
      121 ttcatagtgc aaatccactc accgggcatc tcaggcagta cgcggcggtc aaagctggcg
      181 ttatgcgcga cataccattc actaccgtaa tagtgtggga tcacatcttc aatccacggt
      241 ttatcggcga ccatggcttc ggtgatgcga tgaatcgcca tcgcttgtgg actaatagga
      301 cgatcggggc gcaccaggtg gctcatgggg ttgacgattt ttccgtcaat gacatcaaca
      361 gaggcaatct caacgatccc tccctgcaaa ccgcaggttt ctgtatcgat aatgcgcaac
      421 atggcatgct ccaggccgaa aaagcctagc gtaatggaat gatatcgcct tgccaaccct
      481 gtgatgaacg ctgaccgact aacaataatg aaccacgatc ggcgcgaacg atgagacgac
      541 catattcatc ccatagtgca ctgttaccac gggcatttgc catcagcacg gcaatcgagt
      601 actgatgtga aaaaaattgt aagcgacggg ccgacgccag caggtcgggt tctcccagac
      661 attgccctgt agtaaacagt gaacaggtcg ggtccatatc cataccttgc ggttgttcat
      721 cgaccaccgt gatggttctg gaacgacgac ccagacatgc accatgactc tgatggtaga
      781 tcccaggcgt ttttcgccac ggtgcgaaca ccgcaattcc acgaataaag cgatcgttat
      841 attcaacggg aaggccggca atgatggtca ttcgccaggt cgttgcggca taacaaagcg
      901 ggtctaacag tgagagatcg ggtggggcag gaagggcacg tcgcgaataa tcacacccca
      961 gtaaagaaag cgatggaaaa actaacagct gacattgttg cctggcggcg gccctcacaa
     1021 actccagatg atgagctacc tgttcggtga gtgaggtttt acgaggctca tattgcgctg
     1081 ctgcaacttt ccagaacgac ataatgactt ccttttcaaa atgactgcaa attatgggtg
     1141 cggtttagaa aatactcata atttagcagg cgttatgtaa gaaagtgtaa ctctgtataa
     1201 gttttattta agtttgggct cgtaaggtaa acgtgacaga ttgaccgaag ccaaacggtg
     1261 ggcaataagc cgctcgcgaa accagctgcg caaatgttca ggctgttcac gttcaaccgc
     1321 ttcagcgatc accggcatat tgtagcgttc tttaaatgcc accccggccg ccgccaaatc
     1381 gacattcact ttatccattt ctgtttgatc cagtttagcc agattcttca gcattcttaa
     1441 tctcctcatc attcgcgggc aaaagttact aagagtgagc caggatggca agccggaatt
     1501 gatgatcctc gactcagaca ttagacaagg ttatgctctg taaaaaagtt attacaaaaa
     1561 ggaatagata atatgacttc aactgcacgc tcccttcgct acgcgctggc aatcctgacc
     1621 acttcgttgg tgacaccttc cgtctgggca catgcgcatt taacgcatca gtatcctgcg
     1681 gcaaacgcgc aagtgacagc tgcaccgcag gcaatcactt taaacttctc ggaaggtgtt
     1741 gaaacaggat tcagtggtgc aaaaatcacg gggccaaaaa acgaaaatat taaaacatta
     1801 ccggcgaagc gaaatgagca ggaccaaaag caactgattg ttccactggc cgattcgctg
     1861 aaacccggaa cctacatcgt tgactggcat gttgtttcgg tggacgggca taaaacgaaa
     1921 ggacactaca cctttagcgt gaaataaatc atgctggcgt ttacctggat cgcgctgcga
     1981 tttatccatt tcacctcgct gatgctggtc tttggcttcg caatgtacgg tgcctggctg
     2041 gcccccttaa cgattcgtcg cttgctggcg aagcgttttc tgcgtttaca acagcacgct
     2101 gccgtctgga gtttgatcag tgccactgca atgctcgccg ttcagggcgg gttgatggga
     2161 acgggatggg cagatgtatt ttcgccaaac atttggcagg ctgttttgca aacgcagttt
     2221 ggtggcgtct ggttatggca aattgttctc gccctcgtaa cgttgatcgt cgcccttatg
     2281 caaccgcgga atatgccacg tttgctgttt atgctcacta ccgcgcaatt tattctgctg
     2341 gcgggaatcg ggcatgcgac actgaatgaa ggggtaacgg cgaaaatcca tcagactaat
     2401 cacgcgattc acctgatttg tgcggcggcg tggtttgggg gattactgcc ggtgctctgg
     2461 tgtatgcaac tcatcaaagg ccgttggcga caccaggcca ttcaggcgct gatgcgtttt
     2521 tcctggtgcg ggcattttgc ggtgatcggc gtactggcaa gcggcgtgct taatgcattg
     2581 ttgattaccg gatttccccc cacgcttacg acttactggg gccaactctt attgctcaaa
     2641 gccatcctgg tgatgattat ggtggtcatt gcgctggcta atcgttatgt tcttgtaccg
     2701 cgcatgaggc aagatgaaga tcgtgcggca ccctggtttg agtggatgac taaactcgaa
     2761 tgggcgatag gtgcggtagt gctggtgatc atcagcctgc ttgcgactct cgaacctttt
     2821 tgatggatta cgaaaatgat gaaaaaaagt attctgacgt ttctgttact caccagttct
     2881 gcagcggcgc tggctgcacc gcaggtgatt accgtcagtc gttttgaagt gggtaaagac
     2941 aaatgggcgt ttaatcgcga agaggtgatg ctgacttgca gaccgggtaa tgctttgtat
     3001 gtcattaacc caagtaccct cgtgcagtat cctttaaacg atatcgcaca aaaggaagtt
     3061 gccagtggga agactaaagc ccaacccatt tcggtgattc agattgatga tcctaacaat
     3121 cccggcgaaa aaatgagtct ggcaccgttt atagaacgag ctgaaaaact ctgttaatta
     3181 cctaaaatag cctt
//
LOCUS       DAMZET010000298         3193 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.298, whole genome shotgun sequence.
ACCESSION   DAMZET010000298 DAMZET010000000
VERSION     DAMZET010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.298"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..668)
                     /gene="fes"
                     /locus_tag="POB34_004741"
     CDS             complement(<1..668)
                     /gene="fes"
                     /locus_tag="POB34_004741"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HDI5921564.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     PIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQ"
     gene            911..3151
                     /gene="fepA"
                     /locus_tag="POB34_004742"
     CDS             911..3151
                     /gene="fepA"
                     /locus_tag="POB34_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HDI5921565.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPLDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
BASE COUNT          791 a          814 c          886 g          702 t
ORIGIN      
        1 tgctgacgat gggtcagcga agtcagcaca ggccagacgg gcatactttg cgcccaaaat
       61 tcgccatcga gcaaaactgc cagcggacgt tcttctgctg ttacatcgcc ggtggtaaaa
      121 atccatacac gacgtgaatt tttcaaccgt tcacttttcc agataatttc tttggctgct
      181 atttctggcg cttgcggaca atcccatccc ggttgcagag gcgcttgcgg catttcgagt
      241 gcagaaacag cgtgccctcg cccgcctttc cagctttgtg gattcagcgg atcggctatt
      301 gcctggggta atagttttcg ccagccttca cgtaattcga ggcgatcggg ggatggtgca
      361 gaaaaaatgt catcgcgttc ggtgggaata aagcagtagc tgccgcgcca gttggcattg
      421 agttgtgtcg tccactgcca gacatcagta cctgcaattc gctgcatcga ctggggctgg
      481 ctgttctgat gatgatcggt cacaccagtg atgtagatcc atacgcgctt tatcggcgag
      541 tattcttcag aaccttgggg atcacgccac cagaaagtga cctcaaacat ttcgtcattc
      601 agacgctgcc attccgggcc atgtttcgac tgccaccagc tctcacttcc tacttttaac
      661 gccgtcacac cataacccca tgtttactgt gcaatttttc attgattgca gaaatatatt
      721 gataatatta ttgataacta tttgcatttg caatagcgta atggcgcgcc gtgggaagcg
      781 cggacattta ttaaccaact gcactgcgtg tctttcagga tcaaaggttt tcgcggtagc
      841 gggatgcgtc gtgttgagga cgaccatgcc cgacagttgc aattcgtggc aaaaatgcag
      901 gaataaaaca atgaacaaga agattcactc cctggccttg ttggtcaatc tggggattta
      961 tggggtagcg caggcacaag agccgaccga tactcctgtt tcacatgacg ataccattgt
     1021 cgttaccgcc gccgagcaga acttgcaggc acctggcgtt tcgaccatca ccgcagatga
     1081 aatccgcaaa aaccccgttg cccgcgatgt gtcggagatc atccgtacca tgccaggcgt
     1141 taacctgacc ggtaactcca ccagtggtca gcgtgggaat aaccgacaga ttgatattcg
     1201 cggtatgggt ccggaaaaca cgctgatttt gattgacggc aagccggtaa gcagccgtaa
     1261 ctcggtgcgt cagggctggc gtggcgagcg cgatacccgt ggtgatacct cctgggtgcc
     1321 acctgaaatg attgaacgta ttgaagttct gcgtggtccg gcagctgcgc gttatggcaa
     1381 cggcgcggcg ggcggcgtgg ttaacatcat taccaaaaaa ggcagcggtg agtggcacgg
     1441 ctcctgggac gcttatttca atgcgccaga acataaagag gaaggtgcca ctaaacgcac
     1501 caacttcagc ctgaccggtc cgctgggcga cgaattcagc ttccgcttgt atggcaacct
     1561 cgacaaaacc caggctgacg cgtgggatat caaccagggc catcagtccg cgcgtgccgg
     1621 aacgtatgcc acgacgttac cagccgggcg cgaaggggtg atcaacaaag atattaatgg
     1681 cgtggtgcgc tgggacttcg cgcctctgca atcactcgaa ctggaggcgg gttacagccg
     1741 ccagggtaac ctgtatgcgg gcgataccca gaacaccaac tctgacgctt atactcgctc
     1801 gaaatatggc gatgaaacca accgcctgta tcgccagaac tactcgctga cctggaacgg
     1861 tggctgggat aacggtgtga ccaccagcaa ctgggtgcag tacgaacaca cccgtaactc
     1921 gcgtattccg gaaggtctgg cgggcggtac cgaagggaaa tttaacgaaa aagcgacaca
     1981 ggatttcgtc gatatcgatc ttgatgacgt gatgctgcac agtgaagtta acctgccgct
     2041 tgatttcctc gttaaccaga cgctgacgct gggtacggag tggaatcagc aacggatgaa
     2101 agacttgagt tccaataccc aggcgctgac cgggacgaac accggtggcg ctattgatgg
     2161 cgtgagtgcc accgaccgta gcccgtattc aaaagcagaa attttctcgc tgtttgccga
     2221 aaacaacatg gagctgactg acagcaccat cgtaacgccg gggctgcgtt tcgatcatca
     2281 cagtattgtc ggcaataact ggagcccggc gctgaacata tcgcaaggtt taggcgatga
     2341 cttcacgctg aaaatgggca tcgcccgtgc ttataaagcg ccgagcctgt accagactaa
     2401 cccgaactac attctctaca gtaaaggtca gggttgctat gccagcgcgg gcggctgcta
     2461 tctgcaaggt aacgatgacc tgaaagcaga aaccagcatc aacaaagaga ttggtctgga
     2521 gttcaaacgc gacggctggc tggcgggcgt cacctggttc cgtaacgatt atcgcaataa
     2581 gattgaagcg ggctatgtgg ctgtagggca aaacgcagtc ggcaccgatc tctatcagtg
     2641 ggataacgtg ccgaaagcgg tggttgaagg tctggaagga tcgttaaacg taccggttag
     2701 cgaaacggtg atgtggacca ataacatcac ttatatgctg aagagtgaaa acaaaaccac
     2761 gggcgaccgt ttgtcgatca tcccggagta tacgttgaac tcaacgctga gctggcaggc
     2821 acgggaagat ttgtcgatgc aaacgacctt cacctggtac ggcaagcagc agccgaagaa
     2881 gtacaactat aaaggtcagc cagcggttgg accggaaacc aaagaaatta gtccttacag
     2941 cattgttggc ctgagcgcga cctgggatgt gacgaagaat gtcagtctga ccggcggcgt
     3001 ggacaatctg ttcgacaaac gtttgtggcg tgcgggtaat gcccagacca cgggcgattt
     3061 ggcaggggcc aactatatcg ccggtgccgg ggcgtatacc tataacgagc cgggacgtac
     3121 gtggtatatg agcgtaaaca cccacttctg atgctaacgt cagattgttg acatagtgcg
     3181 cgtcgttcat gcc
//
LOCUS       DAMZET010000299         3188 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.299, whole genome shotgun sequence.
ACCESSION   DAMZET010000299 DAMZET010000000
VERSION     DAMZET010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.299"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..877
                     /locus_tag="POB34_004743"
     CDS             <1..877
                     /locus_tag="POB34_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDI5921566.1"
                     /translation="GKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPARVA
                     YLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYTRR
                     NGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNKNG
                     VYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQVEK
                     WVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAATNK
                     KTLADYARALSGEDE"
     gene            874..1080
                     /locus_tag="POB34_004744"
     CDS             874..1080
                     /locus_tag="POB34_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HDI5921567.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            1077..1148
                     /locus_tag="POB34_004745"
                     /pseudo
     CDS             1077..1148
                     /locus_tag="POB34_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            1145..1411
                     /locus_tag="POB34_004746"
                     /pseudo
     CDS             1145..1411
                     /locus_tag="POB34_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1427..1503)
                     /locus_tag="POB34_004747"
     tRNA            complement(1427..1503)
                     /locus_tag="POB34_004747"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1467..1469),aa:Arg,seq:tct)
     gene            1746..2378
                     /gene="fimZ"
                     /locus_tag="POB34_004748"
     CDS             1746..2378
                     /gene="fimZ"
                     /locus_tag="POB34_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HDI5921568.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSKTLNYIKSNKCSTNSSTITVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(2381..2896)
                     /gene="sfmF"
                     /locus_tag="POB34_004749"
     CDS             complement(2381..2896)
                     /gene="sfmF"
                     /locus_tag="POB34_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HDI5921569.1"
                     /translation="MRRALFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(2907..>3188)
                     /locus_tag="POB34_004750"
     CDS             complement(2907..>3188)
                     /locus_tag="POB34_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI5921570.1"
                     /translation="TNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIVADQNDTPITP
                     NDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDYQ"
BASE COUNT          873 a          757 c          803 g          755 t
ORIGIN      
        1 gggaaaacgt aaaaccttcg tgaacaccac gctcggtgag acatgggaag cgaaaattgg
       61 cgaacgtccg gatgctgagg tgatggcgga gcggaaagag cattattcag cgcccgttcc
      121 tgcccgtgtg gcttacctga ccgccggtat cgactcccag ctggaccgct acgaaatgcg
      181 cgtatgggga tgggggccgg gtgaggaaag ctggctgatt gaccggcaga ttattatggg
      241 ccgccacgac gatgaacaga cgctgctgcg tgtggatgag gccatcaata aaacctatac
      301 ccgccggaat ggtgcagaaa tgtcggtatc ccgtatctgc tgggatactg gcgggattga
      361 cccgaccatt gtgtatgaac gctcgaaaaa gcatgggctg ttccgggtga tccccattaa
      421 aggggcatcc gtctacggaa agccagtggc cagcatgcca cgtaagcgaa acaaaaacgg
      481 ggtttacctt accgaaatcg gtacggatac cgcgaaagag cagatttata accgcttcac
      541 actgacgccg gaaggggatg aaccgcttcc cggtgccgtt cacttcccga ataacccgga
      601 tatttttgat ctgaccgaag cgcagcagct gactgctgaa gagcaggtcg aaaaatgggt
      661 ggatggcagg aaaaaaatac tgtgggacag caaaaagcga cgcaatgagg cgctcgactg
      721 cttcgtttat gcgctggcgg cgctgcgcat cagtatttcc cgctggcagc tggatctcag
      781 tgcactgctg gcgagcctgc aggaagagga tggtgcagca accaacaaga aaacactggc
      841 agattacgcc cgtgccttat ccggagagga tgaatgacgc gacaggaaga acttgccgct
      901 gcccgtgcgg cactgcatga cctgatgaca ggaaaacggg tggcaacggt acagaaagac
      961 ggacggcgag tggagtttac ggccacttcc gtgtctgacc tgaaaaaata cattgcggag
     1021 ctggaagtgc agaccggcat gacacagcga cgcaggggac ctgcaggatt ttatgtatga
     1081 aaacgtccac cattcccacc cttctggggc cggacggcat gacatcgctg cgtgaatatg
     1141 ccggttatca gcttgtcgtc gtgcaggaat tgaagatttc cgtttccatg acctcagaca
     1201 cacctgggca agctggctga tccagtcagg cgtcccatta tctgtgcttc aggaaatggg
     1261 cggatgggag tccatagaaa tggttcgtag gtatgctcac cttgcgccta atcatttgac
     1321 agagcatgca aggaaaatag acgacatttt tggtgataat gtcccaaata tgtcccactg
     1381 tggaattatg gaggatataa agaaggcgta actgattgaa ttgtaatggc gcgccctgca
     1441 ggattcgaac ctgcggccca cgacttagaa ggtcgttgct ctatccaact gagctaaggg
     1501 cgcgttgata ccgcaatgcg gtgtaatcgc gtgaattata cggtcaaccc ttgctgagtc
     1561 aatggctttt gatctggttg ctgaacaagt gaacgaccgc gtctgatttt ctgatttatt
     1621 tcgctatagc ggcaaacaaa cacacaccgc tgcgcgtctg aatcaagaaa acccgtattt
     1681 tcatgtatca aagtacaatt taccgcccta acggaaaatt gtccgctcct atgagactgg
     1741 taactatgaa accaacgtcg gtgatcatta tggatactca tcctatcatc agaatgtcta
     1801 ttgaagttct gttgcaaaaa aacagtgaat tgcagattgt cctgaaaacg gatgattacc
     1861 gcataaccat cgattatctc cgaacccgtc ctgttgattt aatcattatg gatatagact
     1921 tgcccggaac agacggtttt accttcctga aaaggatcaa acaaatccag agcacagtga
     1981 aagtgttatt tttatcatcg aaatcagaat gcttttatgc tggcagagcg atacaagctg
     2041 gtgctaacgg ttttgtcagt aaatgcaatg atcagaatga tatttttcat gccgttcaga
     2101 tgatcctctc cggatacacg ttttttccca gcaaaacgct taactatata aaaagcaata
     2161 aatgtagtac gaatagttca acgatcactg tgctatctaa tcgtgaagtg accatattac
     2221 gttatctggt tagcggatta tctaataaag aaattgccga taagttatta cttagcaata
     2281 aaacagttag tgcgcataaa tctaatattt atggcaagct aggtttgcat tcaattgtag
     2341 agcttatcga ctacgccaaa ttatacgaat taatataata ttaattataa ttgatcataa
     2401 atatcgcatc cgctttcgcc acacctggcc gaacaccgct ggctaacgct cgataattgg
     2461 caaaaaagtt tagtgtgaca ttgccatttg catctacttc ctcagtcggg ctcgcctccc
     2521 ccagtgcgag ccgggagcga tcgctattac gtaattcgat ggcgacggtt tgtgccattg
     2581 cgggatcatc cagagccagc aggttggtat cggatgccgg cgttcccgta aataaaatcg
     2641 caactgaacc cggaggacat ccctccagcc gcaggctaaa agggacgagt gccgtggtat
     2701 cgccagcgtt cagtaattgt gtcgtaggcc atctgcctaa atctaccgtc ttatcaatat
     2761 ccgcagtgtt tacggtacag gagaaatcaa caacgttacc gtgcaaattg atattaatcg
     2821 ttcccaaagg gtcagctgcc catccactgg aactccacag taacccgcag aaacagctaa
     2881 agagtgctct tctcattctc cgtacctcat tgataatcga cgcgtaaata ccccagcgcg
     2941 ctaaacggcc cttcggtcgg tttttgaccg gtaatactga taggccaggc gcgaagtgtg
     3001 acattggctg ccgcagctgc atccagacgg aaaggaataa cgctattgag atcgttaggc
     3061 gtgatcggcg tatcgttctg atcggcgaca ataaaaccta aatcctgatt gtccgacacc
     3121 atcgcctgac cagaaacggc actggcttcc agacgcattg ttaaataagc ctgcgcagca
     3181 acattcgt
//
LOCUS       DAMZET010000300         3177 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.300, whole genome shotgun sequence.
ACCESSION   DAMZET010000300 DAMZET010000000
VERSION     DAMZET010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.300"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..517
                     /gene="lpxT"
                     /locus_tag="POB34_004751"
     CDS             <1..517
                     /gene="lpxT"
                     /locus_tag="POB34_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HDI5921571.1"
                     /translation="CSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTAVVLNQLGQ
                     ALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAFMWRYFGKV
                     AGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDRLITFFDKS
                     LPGKNKHFQNK"
     gene            929..1495
                     /gene="mepS"
                     /locus_tag="POB34_004752"
     CDS             929..1495
                     /gene="mepS"
                     /locus_tag="POB34_004752"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HDI5921572.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            1676..>3177
                     /locus_tag="POB34_004753"
     CDS             1676..>3177
                     /locus_tag="POB34_004753"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDI5921573.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHRHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRP"
BASE COUNT          798 a          753 c          737 g          889 t
ORIGIN      
        1 ttgttctctg ctggcgatgg gaatgttgat gctgagtttc tggctgaaag aaaacgcccc
       61 tggcagacga cgtatcgtga ttatgggtct ggtcatgcta ttaactgcag tggtattaaa
      121 ccagctgggt caggcattaa ttcctgtaaa acgggccagc ccaacattga cttttaccga
      181 tattaaccgc gtcagcgaac tgctctctgt tcccacgaaa gatgcctcac gagatagctt
      241 tcccggcgat cacggcatga tgctgcttat tttttcggca ttcatgtggc gttatttcgg
      301 caaagttgca ggccttatcg cccttattat ttttgtggtt tttgcatttc ccagagtaat
      361 gattggcgca cactggttta ctgacatcat tgtcggttcg atgaccgtga tattgatcgg
      421 tttgccctgg gtgttgctga cgccattaag tgatcgatta atcacctttt ttgacaaatc
      481 actaccggga aaaaacaaac atttccaaaa caaataactc acagtaatta acatcatcag
      541 gcttattttt atagtgagga taatcctgat gatgcgcacc gtgctttcat ccatcgcgcg
      601 caaaaatcat tctctaagta aatgaatgga ttgcatgcgt ttcactcaat cgtactttaa
      661 ttgaccaatc ccgcttatta actttctgta tcactttttc ttataaaaaa tcatgtaaaa
      721 ccgctcgcca agaccgcacc aatcgggtaa tctcgaactc gttttgcctc ggcggtagat
      781 tatcctcaca gcatataatt ttgtgcgtta gtccacagat ttggccttaa ggaattgttt
      841 caacatgccc aggtaattag tctcgtgtcg cttggcattt ttttataacg atatttgtcg
      901 ttaaggactt caagggaaaa caaacaacat ggtcaaatct caaccgattt tgagatatat
      961 cttgcgcggg attcccgcga ttgcagtagc ggttctgctt tctgcatgta gtgcaaataa
     1021 caccgcaaag aatatgcatc ctgagacacg tgcagtgggt agtgaaacat catcactgca
     1081 agcttctcag gatgaatttg aaaacctggt tcgtaatgtc gacgtaaaat cgcgaattat
     1141 ggatcagtat gctgactgga aaggcgtacg ttatcgtctg ggcggcagca ctaaaaaagg
     1201 tatcgattgt tctggtttcg tacagcgtac attccgtgag caatttggct tagaacttcc
     1261 gcgttcgact tacgaacagc aggaaatggg taaatctgtt tcccgcagta atttgcgtac
     1321 gggtgattta gttctgttcc gtgccggttc aacgggacgc catgtcggta tttatatcgg
     1381 caacaatcag tttgtccatg cttccaccag cagtggtgtt attatttcca gcatgaatga
     1441 accgtactgg aagaagcgtt acaacgaagc acgccgggtt ctcagccgca gctaataaac
     1501 cgtttggatg caatcccttg gctatcctga cgagttaact gaaagcactg cttaggcagt
     1561 gcttttttgt tttcattcat cagagaaaat gatgtttccg cgtcttgatc caggctatag
     1621 tccggtcatt gttatctttt aaatgttgtc gtaatttcag gaaattaacg gaatcatgtt
     1681 catacgcgct cccaattctg gacgtaagct cctgcttacc tgcattgttg caggcgtgat
     1741 gattgcgata ctggtgagtt gccttcaatt tttagtggcc tggcataggc acgaagtcaa
     1801 atacgacaca ctgattaccg acgtacaaaa gtatctcgat acctattttg ccgatctgaa
     1861 atccactact gaccggctcc agccgctgac cttagatacc tgccagcaag ctaaccccga
     1921 actgaccgcc cgcgcggcgt ttagcatgaa tgtccgtacg tttgtgctgg tgaaagataa
     1981 aaaaacattc tgttcatctg cgaccggtga gatggacatt ccactcaatg aattgattcc
     2041 ggcgctcgac attaataaaa acgtcgatat ggcgatctta cccggcacgc cgatggtgcc
     2101 gaacaaaccc gcaatcgtca tctggtatcg caaccctttg ctgaaaaata gcggcgtctt
     2161 tgccgctctg aatctcaacc tgacgccttc tctcttttat agttcacggc aggaagatta
     2221 cgatggcgtc gccctcatta ttggtaatac tgcgctatct accttttctt cacgtttgat
     2281 gaacgttaac gaattaaccg acatgccagt ccgtgaaact aaaattgcgg gcattcctct
     2341 gaccgttcgg ctttatgcag atgactggac atggaacgat gtgtggtacg catttttact
     2401 gggcggcatg agtggaactg tcgttggcct gctctgctat tacctgatga gcgtacgtat
     2461 gcgccccggc agagaaatca tgaccgccat caagcgcgaa caattttacg ttgcgtatca
     2521 accggtggtg gatacacaag ctttgcgagt aacgggcctg gaagtactgc tacgctggcg
     2581 gcatcctgta gcgggagaaa ttcccccgga tgccttcatt aactttgccg aatcgcaaaa
     2641 gatgattgtg ccgctgactc agcacctgtt tgagttgatt gcccgcgatg ccgcagaatt
     2701 agaaaaagta ctgccggtag gcgtcaaatt tggcattaac attgcgccgg accatctgca
     2761 tagcgaaagc tttaaagctg atatccagaa actgctcacg tccttgcccg cacaccattt
     2821 ccagattgtg ctggaaatta ccgagcgcga tatgctgaaa gagcaagaag ccacacaact
     2881 cttcgcctgg ctgcactcgg tcggcgtaga aattgctatt gatgacttcg gcaccgggca
     2941 cagcgcgctt atctatcttg agcgttttac gctcgattat ctgaaaattg accgtggatt
     3001 tatcaacgcc atcggtacgg aaacgatcac ttcccccgta cttgacgcgg tgctgacgct
     3061 ggcgaaacgc ctcaatatgc tgacggttgc tgaaggggtc gaaacgccgg aacaggcgcg
     3121 atggctaagc gaacgcggcg ttaatttcat gcaaggctac tggattagtc gcccgtt
//
LOCUS       DAMZET010000301         3161 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.301, whole genome shotgun sequence.
ACCESSION   DAMZET010000301 DAMZET010000000
VERSION     DAMZET010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.301"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            1..1002
                     /gene="ltaE"
                     /locus_tag="POB34_004754"
     CDS             1..1002
                     /gene="ltaE"
                     /locus_tag="POB34_004754"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDI5921574.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVEEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            1013..2443
                     /locus_tag="POB34_004755"
     CDS             1013..2443
                     /locus_tag="POB34_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HDI5921575.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPERKYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            2542..>3161
                     /locus_tag="POB34_004756"
     CDS             2542..>3161
                     /locus_tag="POB34_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDI5921576.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMT"
BASE COUNT          711 a          847 c          883 g          720 t
ORIGIN      
        1 atgattgatt tacgcagtga taccgttacc cgaccgagcc gcgccatgct cgaagcgatg
       61 atggccgccc cggttgggga cgacgtttac ggagacgacc ctaccgttaa tgctctgcag
      121 gactatgcag cagagctttc cggtaaagaa gccgccattt ttctgcctac cggcactcag
      181 gccaacctgg tcgctctgct cagtcactgc gaacgtggcg aagagtatat tgtcggtcag
      241 gccgcgcata actatctgtt tgaagccggt ggcgcagcgg tgctgggcag tattcagccg
      301 caacccatcg acgcggctgc cgacggcacg ctaccgctgg ataaagtggc gatgaaaatc
      361 aaacccgacg atatccattt cgcccgcacc aaattactca gtctggaaaa cacccacaac
      421 ggcaaagtgc tgccgcgtga atacctgaaa gaagcatggg aatttacccg cgagcgcaat
      481 ctggcgctgc atgttgacgg tgcgcgcatc tttaatgccg tggtggctta cggctgcgaa
      541 ctgaaagaga tcacgcaata ttgtgattcg ttcaccattt gcctgtcgaa aggtcttggg
      601 acgccagtcg gttcattact cgtcggtaat cgtgattaca ttaaacgtgc catccgctgg
      661 cggaaaatgg ccggtggcgg gatgcgtcag tccgggattc tggctgccgc cgggatgtat
      721 gccctgaaaa ataacgtcgc acggttgcag gaagatcacg acaacgccgc ctggatggcg
      781 gagcaactgc gtgaagcagg cgcggatgtg atgcgtcagg acactaatat gctgtttgtt
      841 cgcgtcgagg aagaaaatgc tgccgcgtta ggcgaataca tgaaagcgag aaacgtgctg
      901 attaacgcct cgccgattgt ccgcctggtg acgcatcttg acgtctcgcg cgaacaactg
      961 gcggaagtcg ccgcccactg gcgcgcattc ctggcgcgtt aaggagagag acgtgccgca
     1021 acgcatttta gttctcggtg ccagtggcta cattggtcag catctggtgc gcacactcag
     1081 ccagcaaggg catcagatcc tggcggcggc acgtcatgtc gacaggcttg caaagctgca
     1141 actggcaaac gtcagttgcc ataaagtcga tctcagctgg ccggataacc ttccggccct
     1201 gttgcaggat atcgataccg tctattttct ggtgcacagc atgggcgaag gcggcgattt
     1261 tatcgctcag gagcgccagg tggctctcaa cgtccgcgat gcgctacgtg aagtaccagt
     1321 taagcaatta atctttctca gttcgttgca ggccccgcca catgagcagt cggatcatct
     1381 gcgtgctcgt caggctacgg cggacattct tcgtgaagcg aatgtacctg tgaccgaact
     1441 tcgggccgga attatcgttg gcgcaggttc agcggcgttc gaagtcatgc gcgatatggt
     1501 ctacaacctg ccagtgttaa cgccgccacg ctgggtacgt tcacgcacca cgcccatcgc
     1561 gctggaaaac ttgctgcact atctggtggc gttgttagat catccggcca gcgaacaccg
     1621 catcttcgaa gccgccggac cagaggtgct cagttatcag caacagtttg aacattttat
     1681 ggcggtgagc ggtaagcgcc gctggttgat ccccatcccc ctccccaccc gctggatttc
     1741 ggtgtggttt ctcaatgtga ttacttccgt accgcccacc accgccaggg cgttgattca
     1801 ggggctgaaa cacgatctgc tggcggatga taccgcgcta cgtgcactca tcccacaacg
     1861 gctgatcgct ttcgatgacg cggtacgtag cacgttgaaa gaggaggaaa aactggtcaa
     1921 ctccagcgac tggggctacg acgctcaggc ctttgcccgc tggcgaccgg agtacggtta
     1981 ttttgccaaa caggcggggt ttaccgttaa aacgtccgcc agccttgctg ctttatggca
     2041 ggtagtgaac caaatcggcg gtaaagagcg ttatttcttt ggcaatattt tgtggcagac
     2101 acgggcgttg atggaccgcg cgatcggtca taagctggcg aaaggccgcc cggagcgcaa
     2161 atatttacag actggcgatg cggtagatag ctggaaagtg attgtcgttg aaccggaaaa
     2221 acaacttacg ttgttatttg gcatgaaagc gccggggctg ggacgactgt gttttagcct
     2281 ggaagataaa ggcgactatc gtactatcga tgtccgcgct ttctggcatc cgcacggtat
     2341 gccggggctg ttttactggt tattgatgat ccccgcgcat ctgtttattt ttcgcggaat
     2401 ggcaaaacaa atcgccagac tggcagaaca aagcacagat taatcgacaa aacctgcgtt
     2461 attctttcat gtttcccatt gcatcatggg ggaaatcacg gaagaatgcg cacgttattt
     2521 tctcaacaac agtggattga tatgaaggta ctggttaccg gcgccaccag cggcttaggt
     2581 cgaaacgcgg tagagttttt atgccagaaa ggcatcagcg tgcgagcgac cggtcgcaac
     2641 gaggcaatgg gcaaattgct ggaaaaaatg ggcgcagagt ttgttccggc ggatctgacc
     2701 gagctggttt catcacaagc taaagtgatg ctcgcgggca ttgatacgct gtggcactgc
     2761 tccagcttta cctcaccctg ggggacacaa caggctttcg atctggctaa cgttcgcgcc
     2821 actcgccgcc tgggtgaatg ggctgtcgcc tggggtgtgc gtaactttat tcatatctct
     2881 tccccctccc tgtacttcga ttatcaccac catcgcgata ttaaagaaga ttttcgccct
     2941 caccgcttcg ccaacgagtt tgcccgcagc aaagcagcca gcgaagaagt gatcaatatg
     3001 ctttcgcagg cgaatccaca aacgcgcttt actattctgc gcccacaaag tctgttcgga
     3061 ccgcacgata aagtctttat tccccgtctg gcgcatatga tgcaccacta cggcagtatt
     3121 ctgttaccgc atggcggcag tgcgctggtc gatatgacct a
//
LOCUS       DAMZET010000302         3144 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.302, whole genome shotgun sequence.
ACCESSION   DAMZET010000302 DAMZET010000000
VERSION     DAMZET010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.302"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..544
                     /gene="dcm"
                     /locus_tag="POB34_004757"
     CDS             <1..544
                     /gene="dcm"
                     /locus_tag="POB34_004757"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HDI5921577.1"
                     /translation="DFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYRYA
                     KKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDPQN
                     QQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLLEP
                     KIKQAVALRQQEAQHGRRSR"
     gene            525..995
                     /gene="vsr"
                     /locus_tag="POB34_004758"
     CDS             525..995
                     /gene="vsr"
                     /locus_tag="POB34_004758"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HDI5921578.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(984..1904)
                     /gene="yedA"
                     /locus_tag="POB34_004759"
     CDS             complement(984..1904)
                     /gene="yedA"
                     /locus_tag="POB34_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HDI5921579.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            2077..2994
                     /locus_tag="POB34_004760"
     CDS             2077..2994
                     /locus_tag="POB34_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HDI5921580.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            3073..>3144
                     /locus_tag="POB34_004761"
     CDS             3073..>3144
                     /locus_tag="POB34_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HDI5921581.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYS"
BASE COUNT          787 a          766 c          900 g          691 t
ORIGIN      
        1 cgattttacc ctgcgtgata tcagcgaatg tttccctgcg cagcgagtga cgctggcgca
       61 gctgctggac ccgatggtcg aggcgaaata tatcctgacg ccggtgctgt ggaagtacct
      121 ctatcgatat gcgaaaaaac atcaggcgcg cggtaacggc ttcggttatg gaatggttta
      181 tccgaacaat ccgcaaagcg tcacgcgtac gctgtctgcg cgttattaca aagatggcgc
      241 ggaaatttta atcgatcgcg gctgggatat ggccaaaggt gagaaagact ttgacgatcc
      301 gcagaatcag caacatcgtc cacgtcggtt aacgcctcgt gaatgcgcgc gcttaatggg
      361 ttttgaagcg ccgggagaag cgaaattccg cattccggtt tcggacactc aggcctatcg
      421 ccagttcggt aactcggtgg tcgtgccggt ctttgccgcg gtggcaaaac tgcttgagcc
      481 aaaaatcaaa caggcggtgg cgttgcgtca gcaagaggca caacatggcc gacgttcacg
      541 ataaggccac tcgcagcaaa aatatgcgcg cgattgccac gcgtgatacg gcgattgaga
      601 agcgcctcgc cagtctgtta accgggcagg gcctggcgtt tcgcgttcag gacgccagtc
      661 tgcccggacg cccggatttt gtcgttgatg aatatcgttg cgtgatattt acccacggct
      721 gcttctggca tcatcatcac tgctatctgt ttaaagtgcc tgcgactcga accgagttct
      781 ggctggagaa gataggtaaa aatgttgagc gcgatcgccg tgatatcagt cgcttgcagg
      841 agcttggctg gcgcgtactg attgtctggg agtgtgcgtt acgtgggcgc gagaagctga
      901 cggatgaagc gcttagcgag cgtctggaag agtggatctg cggcgaaggt gccagcgcgc
      961 agatcgacac gcaggggatt catttactcg cttgatgcgt cctgaatcac tggcgcaact
     1021 acgggttttg ccggaaagag atattttccc aacgtgacca gcaccaccgc gaagacaatt
     1081 acgccgagcg ccagccattc aatcttcgac agcgtttctc cgcccagtcc cgtacccagc
     1141 aagaccgcga ccaccgggtt aacgtaggcg tagctggtgg cgagagccgg actgacatta
     1201 cggattaaat acatataagc gttaatggcg ataatcgaac caaacagcgc cagatagccg
     1261 accgcaagga agcctgaaag ggaagggagc gccgtcagtt tttcacctgc aatcatcgac
     1321 gcgatcatta acaccacgcc tgccgccagc atctcaatcg cacccgccat catccctaca
     1381 ggtaaggtaa tgcgcgagcc ataaaccgag ccaaacgccc agctaatcga gccgattaaa
     1441 atcagaatcg cgccccacgg attgccgctt aaatttccac cgctattgag catgatgatt
     1501 ccggcaagcc caatggcgat acccacccat tccagtttgc gcgttttaat gccaaacagg
     1561 cggctgaagc acagggtaaa gaggggcacg gttgcaacca caacggcggc gatgccggaa
     1621 ggaacatttt gatgctcggc aaccgtcacc atgccattac cgacagccag caataacagg
     1681 ccaatcagcg cggcattgag cagcggacgt agcgggggga gcttgtgtcc gcgcagtagc
     1741 aaaaatgcca tcaataaaat accggctgcc aggaatcgaa cgcctgccat cattaacgga
     1801 ggccagcttt ccacgccaat ccgaatgaca aaataggttg agccccaaat gatatacaac
     1861 gcaaacagcg cgccaaaaag cggtaacaac tggcggaaac gcataatccc tcacggtgga
     1921 aataaaaagg tggttcatag taaacgtgaa aatcattctg ctggcgagag atataattgc
     1981 acttgattgt taaaaaaatg ttgacctgtg aagcaagtat cagagctgcc gtttttgctt
     2041 catacttaca cccttcaaca ataaaaatga gagggaatgc ttttggccgg gagtagttta
     2101 ctgacgttgc tcgatgatat cgccacacta ctggacgata tctccgtgat gggcaaactg
     2161 gcggcgaaga aaaccgccgg tgtattaggg gatgacttat cgctcaatgc gcaacaagtt
     2221 tcaggcgtgc gggccaaccg ggaacttccc gtggtctggg gcgtggcgaa aggatcgctg
     2281 attaataaag tgattctggt gccgctggcg ctgatcatca gtgcgtttat cccgtgggcg
     2341 attacgcctc tgttgatgat tggtggcgcg tttctctgct ttgaaggagt agagaaagtg
     2401 ctgcatatgc tggaggcgcg taaacataaa gaagatccgg cgcagagcca gcagcgtctg
     2461 gagaagctgg cggcgcagga tccgctgaag tttgaaaagg acaaaataaa aggggcgatt
     2521 cgtaccgatt ttatattgtc tgccgaaatc gtcgccatca cgctggggat tgtggccgaa
     2581 gcgccgttgc ttaatcaggt gctggtgctt tcaggcatcg cgctggtagt gaccgtgggc
     2641 gtctacggtc tggtaggggt tatcgttaag attgatgacc tggggtattg gctggcggaa
     2701 aaatccagcg cgctgatgca ggcattaggt aaagggttat tgatcattgc gccctggttg
     2761 atgaaggcgt tatcgattgt cggcacgctg gcgatgttcc tcgtcggcgg tgggattgtg
     2821 gtacatggaa ttgcgccgct gcatcacgcc attgaacatt tcgccgggca gcaaagtgca
     2881 gtggtggcga tgatattacc gactgtttta aatctgattc ttggatttat catcggcggc
     2941 atcgtggtgc tgggagtgaa agccgtagcg aaaatacgcg gtcaggcaca ttaatactcg
     3001 cacaggctat tatgcaaaat tcgccatctt caactaaggt gaacaagttt cactcgaaaa
     3061 aaggaggcga gtatgagctt tatggttagt gaggaagtta cggttaaaga gggcggcccg
     3121 cggatgattg tcaccggata ctcc
//
LOCUS       DAMZET010000303         3102 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.303, whole genome shotgun sequence.
ACCESSION   DAMZET010000303 DAMZET010000000
VERSION     DAMZET010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.303"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..221
                     /locus_tag="POB34_004762"
     CDS             <1..221
                     /locus_tag="POB34_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDI5921582.1"
                     /translation="LSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLL
                     EDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            377..1912
                     /gene="gadC"
                     /locus_tag="POB34_004763"
     CDS             377..1912
                     /gene="gadC"
                     /locus_tag="POB34_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HDI5921583.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2043..>3102
                     /locus_tag="POB34_004764"
     CDS             2043..>3102
                     /locus_tag="POB34_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HDI5921584.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDV"
BASE COUNT          733 a          780 c          769 g          820 t
ORIGIN      
        1 acctctctga acgtctgcgt ctgcgcggct ggcaggttcc ggccttcact ctcggcggtg
       61 aagccaccga catcgtggtg atgcgcatta tgtgtcgtcg cggcttcgaa atggactttg
      121 ctgaactgtt gctggaagac tacaaagcct ccctgaaata tctcagcgat cacccgaaac
      181 tgcagggtat tgcccagcag aacagcttta aacatacctg ataacgttta acggtaacgg
      241 tgtcccgaaa cgaacccgtt tcgggacaat ttccaaagtc tgttcactgg cattagcaac
      301 ggaaaatatt gttctgaata cgcttcagaa caaaacaggt gcggttccga caggaatacc
      361 gttttagggg gataatatgg ctacatcagt acagacaggt aaagctaagc agctcacatt
      421 actcggattc tttgccataa cggcatcgat ggtaatggct gtttatgaat accctacctt
      481 cgcaacatcg ggcttttcat tagtcttctt cctgctatta ggcgggattt tatggtttat
      541 tcccgtggga ctttgtgctg cggaaatggc caccgtcgac ggctgggaag aaggtggtgt
      601 cttcgcctgg gtatcaaata ctctggggcc gagatgggga tttgcagcga tctcatttgg
      661 ctatctgcaa atcgccattg gttttattcc gatgctctat ttcgtgttag gggcactctc
      721 ctacatcctg aaatggccag cgctgaatga agaccccatt accaaaacta ttgcagcact
      781 catcattctt tgggcgctgg cattaacgca gtttggtggc acgaaataca cggcgcgaat
      841 tgctaaagtt ggcttcttcg ccggtatcct gttacctgca tttattttga tcgcattagc
      901 ggctatttat ctgcactccg gtgcccccgt tgctatcgaa atggattcga agaccttctt
      961 ccctgacttc tctaaagtgg gcacactggt tgtatttgtt gccttcattt tgagttatat
     1021 gggcgtagaa gcttccgcaa ctcacgtcaa tgaaatgagc aaccccgggc gcgactatcc
     1081 actggctatg ttactgctga tggtggcggc aatctgctta agctctgttg gcggtttatc
     1141 tattgcgatg gtcattccgg gtaatgaaat caacctctcc gcaggggtaa tgcaaacctt
     1201 taccgttctg atgtcccatg tggcaccgga aattgagtgg acggttcgcg tgatctccgc
     1261 actgctgttg ctgggtgttc tggcggaaat cgcctcctgg attgttggtc cttctcgcgg
     1321 gatgtatgtc acagcgcaga aaaacctgct gccagcggca ttcgctaaaa tgaacaaaaa
     1381 tggcgtaccg gtaacgctgg tcatttcgca gctggtgatt acttctatcg cgttgatcat
     1441 cctcaccaat accggtggcg gtaacaacat gtccttcctg atcgcactgg cgctgacggt
     1501 ggtgatttat ctgtgtgctt atttcatgct gtttattggc tacattgtgt tggttcttaa
     1561 acatcctgac ttaaaacgca catttaatat ccctggtggt aaaggggtga aattggtcgt
     1621 ggcaattgtc ggtctgctga cttcaattat ggcgtttatt gtttccttcc tgccgccgga
     1681 taacatccag ggtgattcta ccgatatgta tgttgaatta ctggttgtta gtttcctggt
     1741 ggtacttgcc ctgcccttta ttctctatgc tgttcatgat cgtaaaggca aagcgaatac
     1801 cggcgtcact ctggagccaa tcaacagtca gaacgcacca aaaggtcact tcttcctgca
     1861 cccgcgtgca cgttcaccac actatattgt gatgaatgac aagaaacact aaccatgtct
     1921 gaatggttat aagacaaggg agcgataagt catcgctccc tttttcgtgc ttgctgtcgt
     1981 attgacttaa ccgggataag tacgagaatg agcgcacatc tgtttaccgg aaaccagcac
     2041 atatggatat ctgctcccga aacaagaaat taacgattag aagaccagcg atactagttg
     2101 cactggcact tttactgtgt agttgtaaaa gcacgcctcc agagtccatg gtgacaccac
     2161 cagcaggttc aaagccacca gccacgacgc aacaatcgtc acaaccgatg cgtggcatct
     2221 ggctggccac ggtttctcgg ctcgactggc caccggtttc ctcggttaac attagtaacc
     2281 ccaccagccg ggcccgtgta caacaacagg cgatgatcga caaactggat catctgcaac
     2341 gtctcggcat aaacacggtc tttttccagg tcaagccgga cggtaccgcc ctgtggccat
     2401 cgaaaatttt gccgtggtcc gatcttatga ccggtaagat tggtgaaaat ccgggttacg
     2461 atccgctgca atttatgctc gacgaagccc acaagcgtgg gatgaaagta cacgcctggt
     2521 ttaaccccta tcgcgtatcg gttaatacga agcccggtac tatcagggaa ctgaatagca
     2581 ctctgtctca acaaccggcg agcgtctatg tgcaacaccg cgactggatc agaacgtctg
     2641 gcgatcgctt tgtcctcgac ccgggcatcc ctgaggttca ggactggatc acatcaatag
     2701 tcgcagaagt ggtttcccgc tatcccgtag atggcgtgca gtttgacgac tatttctata
     2761 cggagtcacc gggttcacgg ctaaatgata acgaaacgta ccgtaaatac ggaggcgcat
     2821 ttgcgtcaaa agcagactgg cggcgcaaca atactcagca gttaattgca aaggtatcgc
     2881 acaccattaa aagcattaag ccgggagtcg aatttggtgt tagcccggca ggcgtgtggc
     2941 gtaaccgatc acacgatccg ctcggttccg atacccgagg cgcggcagcc tatgacgaat
     3001 cctacgctga cacccgtcga tgggtggaac aaggattgct ggattacatt gctccccaaa
     3061 tttactggcc gttctcacgg agtgccgcgc gttatgacgt gt
//
LOCUS       DAMZET010000304         3063 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.304, whole genome shotgun sequence.
ACCESSION   DAMZET010000304 DAMZET010000000
VERSION     DAMZET010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.304"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            61..1935
                     /gene="htpG"
                     /locus_tag="POB34_004765"
     CDS             61..1935
                     /gene="htpG"
                     /locus_tag="POB34_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDI5921585.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSGITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            2116..2760
                     /gene="adk"
                     /locus_tag="POB34_004766"
     CDS             2116..2760
                     /gene="adk"
                     /locus_tag="POB34_004766"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDI5921586.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
BASE COUNT          786 a          788 c          799 g          690 t
ORIGIN      
        1 tccccatctc tccctcatcc tgtttttaac cttaaaatgg cattattgag gtagacctac
       61 atgaaaggac aagaaactcg tggttttcag tcagaagtga aacagcttct gcacctgatg
      121 atccattctc tctattccaa taaagaaatc ttcctgcgtg agcttatctc taacgcctcc
      181 gatgcggcgg acaagctgcg tttccgtgcg ctatctaacc cggacctgta cgaaggtgat
      241 ggcgaactgc gcgttcgtgt ctctttcgat aaagacaagc gcacgctgac catctccgat
      301 aacggcgtgg ggatgacccg cgacgaagtg attgaccatc tggggactat cgctaaatcc
      361 ggtaccaaat cattcctcga atccctgggt tctgaccagg cgaaagacag ccagctgatc
      421 ggtcagtttg gtgttggttt ctactctgcg tttatcgtgg ccgacaaagt gaccgtgcgt
      481 actcgcgcgg caggcgaaaa accagaaaac ggcgtcttct gggaatcggc tggcgaaggt
      541 gaatataccg ttgccgacat caccaaagaa gatcgtggta ctgaaatcac cctgcatctg
      601 cgtgaaggcg aagacgagtt cctcgatgac tggcgcgtgc gttccatcat cagcaaatac
      661 tccgaccata tcgcgctgcc ggtagagatc gaaaaacgcg aagagaaaga cggcgaaacc
      721 gttatctcct gggagaaaat caacaaagcg caggcgctgt ggactcgtaa caagtcggga
      781 atcaccgatg aagagtacaa agagttctac aaacacatcg cccacgactt taatgatccg
      841 ctgacctgga gccacaaccg cgttgaaggt aagcaggagt acaccagcct gctgtacatc
      901 ccgtcccagg ctccgtggga tatgtggaac cgtgatcata aacacggcct gaaactgtac
      961 gtccagcgtg tgttcatcat ggacgacgca gaacagttca tgccgaacta tctgcgcttc
     1021 gtgcgtggtc tgattgactc cagcgatctg ccgctgaacg tttcccgtga aatcctccag
     1081 gacagcacgg taacgcgtaa cctgcgcaat gcgctgacca agcgtgtgct gcaaatgctg
     1141 gaaaaactgg cgaaagacga cgcggaaaaa taccagacct tctggcaaca gtttggcctg
     1201 gtactgaaag aaggtccggc ggaagatttc gctaaccagg aagcgatcgc caaactgctg
     1261 cgttttgctt ctacttatac cgattcttct gcgcagaccg tgtctctgga agactacgtt
     1321 tcccgcatga aagaagggca ggagaaaatc tactacatca ctgcagacag ctatgcggca
     1381 gcgaagagca gcccgcacct ggaactgctg cgtaagaaag gcatcgaagt tctgctgctt
     1441 tccgaccgca tcgatgagtg gatgatgaac tatctgactg agttcgacgg taaaccgttc
     1501 cagtcggtgt ctaaagttga cgagtcgctg gaaaaactgg ctgacgaagt tgatgagagc
     1561 gcgaaagaag cggagaaagc actgactccg ttcatcgacc gtgtgaaagc cctgctcggc
     1621 gagcgcgtga aagatgtccg tctgactcac cgtctgaccg atacgccagc gatcgtttcg
     1681 accgacgcgg acgaaatgag cactcagatg gcgaaactgt tcgctgcggc gggccagaaa
     1741 gtgccagaag tgaaatacat cttcgaactg aacccggatc acgtactggt gaaacgtgcg
     1801 gcagatactg aagatgaagc gaagttcagc gagtgggtag aactgctgct ggatcaggcg
     1861 ctgctggcag aacgcggcac gctggaagat ccgaacctgt ttattcgtcg tatgaaccag
     1921 ctgctggttt cctgatgtca tgccggatga cctttgtgtc atccggcatt tttcttttca
     1981 tcatctgcac tttccgcaaa ttatctcgcc attaaccgtt tcagcctcag gtgcctttct
     2041 tgaggcaatc gcctgttggt ggtatcgttt atcgcttttt caaaaaattc gacacatttt
     2101 aaggggattt tcgcaatgcg tatcattctg cttggcgctc cgggcgcggg gaaagggact
     2161 caggctcagt tcatcatgga gaaatatggt attccgcaaa tctccactgg cgatatgctg
     2221 cgtgctgcgg tcaaatctgg ctccgagctg ggtaaacaag caaaagacat tatggatgct
     2281 ggcaaactgg tcaccgacga actggtgatc gcgctggtta aagagcgcat tgctcaggaa
     2341 gactgccgta atggtttcct gttggacggc ttcccgcgta ccattccgca ggcagacgcg
     2401 atgaaagaag cgggcatcaa tgttgattac gttctggaat tcgacgtacc ggacgaactg
     2461 attgttgatc gtatcgtagg ccgccgcgtt catgcgccgt ctggtcgtgt ttatcacgtt
     2521 aaattcaatc cgccgaaagt agaaggcaaa gacgacgtta ccggtgaaga actgactacc
     2581 cgtaaagacg atcaggaaga aaccgtgcgt aaacgtctgg ttgaatacca tcagatgact
     2641 gcaccgctga tcggctacta ctccaaagaa gcggaagcgg gtaacaccaa atacgcgaaa
     2701 gttgacggca ccaagccggt tgctgaagtt cgcgctgatc tggaaaaaat cctcggctaa
     2761 ttcgaaagcg cgcacggaca gtcccctcgc cccctcgggg agagggttag ggtgagggga
     2821 acaggcccgc acaagcaaac ttatcagcaa tctcaggccg gatattcatt cggcctttta
     2881 caaaaacctc aatctgcgct attccttttt ctgatttgac ctctcacagc aattagttct
     2941 tcttcctcac ttttccgcta caattatcaa caagttgaat cgataagagg cggtaatgcg
     3001 tcagactaaa accggtatcc tgctggcaaa cctgggtacg cccgatgccc ccacacctga
     3061 agc
//
LOCUS       DAMZET010000305         3033 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.305, whole genome shotgun sequence.
ACCESSION   DAMZET010000305 DAMZET010000000
VERSION     DAMZET010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.305"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..90
                     /locus_tag="POB34_004767"
     CDS             <1..90
                     /locus_tag="POB34_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 domain-containing protein"
                     /protein_id="HDI5921587.1"
                     /translation="SIMLDMEKLISAYRRMLRQGNHALTVIVG"
     gene            complement(157..1614)
                     /gene="nuoN"
                     /locus_tag="POB34_004768"
     CDS             complement(157..1614)
                     /gene="nuoN"
                     /locus_tag="POB34_004768"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HDI5921588.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYVPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(1621..>3033)
                     /gene="nuoM"
                     /locus_tag="POB34_004769"
     CDS             complement(1621..>3033)
                     /gene="nuoM"
                     /locus_tag="POB34_004769"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HDI5921589.1"
                     /translation="LALSLQLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAI
                     DGLSLLMVVLTGLLGVLAVLCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFF
                     FWEMMLVPMYFLIALWGHKASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNA
                     TGVWTFNYEELLNTPMSSGVEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSV
                     DLAGILLKTAAYGLLRFSLPLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLI
                     AYTSVSHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRM
                     MGGLWSKMKWLPALSLFFAVATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVF
                     ASVYSLAMLHRAYFGKAKSQIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTS
                     HSAIGNIQQWFVNSVTTTRP"
BASE COUNT          841 a          867 c          820 g          505 t
ORIGIN      
        1 tcgatcatgc ttgatatgga aaagctgatt agcgcgtatc gacgtatgct gcgacaaggg
       61 aatcatgcgc tgacggttat tgttgggtga tggtgtcaga gcgccaaaaa aaaccgccga
      121 gcatgtcggc ggctttctga cttacaaact aacagattac atcagcggca ttgctaaccg
      181 cacaatgcta atcagcggtt ggggccatac acccagcacc agtaccaaca gtgcagaaat
      241 cagcaccact ataccgcccg cgctgtactg ccagtttgat ggtgcatcgc gacccggttg
      301 ttccggggcg tgaagataca ggctcaccgc cacgcgcagg tagtagtaga ggccgattgc
      361 cgaaccgaca accacggcac ccaccagcca ccacaagtgt gcctggacac cgaccgccag
      421 cacgtagaac ttaccgataa agcccagcgt catcgggata ccggccagag acagcatcat
      481 caccgtcatc actgccgcga ggatcggacg atgccagaac agaccgcggt aagagaacag
      541 ggaatcagca tccgggccac gatacgggct ggacatcagg ctgaccacgc cgaacgcgcc
      601 gaggctgctg aacagataac cggccaggta aacccctacc gcttccatcg acatctcacc
      661 agtttgcagc gcaatcagcg ccaccagcag atagccgagg tgagagatag atgagtaacc
      721 gagcagacgt ttgatgttgg tctggctcag cgccatcagg ttaccgaaga tgatggaggc
      781 aaaggcgata atcgccagta ctacgcgaat cgcttcgctg tcacccaccg gtacgtagag
      841 gaacagacgc atcaccacac cgaagatagc gattttgctc gccgtcgcca ggaaagtgga
      901 aaccggcgca ggcgcgccct ggtatacgtc tggcgtccac aggtggaacg gcaccagcga
      961 gagtttgaag ccgaggccaa caatcatcag tccgaaacct gccagcaaca gcggctcgtt
     1021 gagcataccg tcgccgaggt ttttacccag cgcgacaaac gacaggtcgc cagactgtgc
     1081 atacaccagc gccataccaa acagcaggaa agaagacgct gcggcagaaa ggatggtgta
     1141 tttgatactg gcttccagcg aacgtttctg gcggaaagcg taaccgacca ggccaaacag
     1201 cggcaaagag atcagttcaa taccgaggaa caaagacgcc agatggttgg cattcgccag
     1261 caggatcccg cccagcgcgg caattaacac caacaggtag aactcatcct tgttgtcgtt
     1321 atagccttca agccacgggt aggcgaaagt acaggtggcg aggctcgcca acaataccag
     1381 cccggtgtaa agcatggcga aaccatcaac gcgcatcagc ggcgtaacgt ccatagcgcc
     1441 cgcctggccg acaaaccaga gcgaaaccag cgccgcgtta agcccaataa ccgagagcgt
     1501 agcgttgagg aaatgattgc gtcgccacgc aatggagagc atcacaacca ccaccgtcaa
     1561 gccgacgatc agcaacggta gcagtgcgat caggttttgt ggagttattg tcatggcgat
     1621 ttacggcctt gtagtagtaa cggaattaac aaaccactgc tggatattgc caatcgcgga
     1681 gtgcgaggta tccagaatcg gctgcggata gaagcccagc agtaccagca gcaccaccag
     1741 caacaggatc ataaacagct cacgcagcga catccctggc agttcctggc tggcaatctg
     1801 gcttttcgct ttaccgaagt aagcgcgatg caacatcgcc agcgaataaa cagatgcaaa
     1861 gaccagccca aaggtagaga taacggtaat caccgggaca acctggaagc tgccgaacag
     1921 aatcataaat tcgccgacga agttaccggt gccaggcatc ccaagcgttg ccaccgcaaa
     1981 gaacagcgac agtgctggca gccatttcat cttgctccac agaccgccca tcatgcgcat
     2041 gtcgcgggta tggatacgtt cataaagctg accacaaaga ataaacagac ccgccgccga
     2101 caagccgtgc gcaatcatct ggattaccgc gccctggtag gccaactggc tgccggtgta
     2161 gatagcaatc agcacgaagc ccatgtggga aaccgaggtg taggcgatca gacgtttgat
     2221 atcggtctgg gcgaaagcca tccacgcacc gtagaagatg ccgataaccc ccagccacat
     2281 agcgattggc gcaaactctg ccgatgcgtt cgggaacagc ggcagggaga aacgcagcaa
     2341 accgtaagcg gcagttttca gcaagatccc cgcgaggtca acggaaccgg cggtcggagc
     2401 ctgagagtgc gcatccggta gccagccatg cagcggaacc accggcattt tgactgcgaa
     2461 ggcgatgaag aagcccagca ttaacaggta ttccacacca ctggacattg gcgtattcag
     2521 cagctcttca tagttgaagg tccagacgcc ggtcgcattg tagtgaacaa aaaccagcgc
     2581 caggatggca atcaacatca ccaggccact cgcctgggtg taaatgaaga acttggttgc
     2641 cgccgtgata cgcgttttac cgtcagaggc tttatgcccc cacagtgcga tcaggaagta
     2701 catcggcacc agcatcattt cccagaagaa gaagaacagg aacatgtcga tggcaaggaa
     2761 cacgccgata acgccgccca ggatccacat caggttgagg tggaagaagc cctgatattt
     2821 ttcgatctct ttccacgaac atagtaccgc cagcacaccg agcagaccgg tcagcacgac
     2881 catcagcagc gacagcccgt caatggcgag atgaatagag ataccaaaac gcgggatcca
     2941 cggcatgtcg aattcagact gccactgcgg aattccggcg gattgcgtca gtgaataacc
     3001 gccctgcaac cacagttgca gcgacagcgc cag
//
LOCUS       DAMZET010000306         2893 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.306, whole genome shotgun sequence.
ACCESSION   DAMZET010000306 DAMZET010000000
VERSION     DAMZET010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.306"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(474..1445)
                     /locus_tag="POB34_004770"
     CDS             complement(474..1445)
                     /locus_tag="POB34_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HDI5921590.1"
                     /translation="MKRAPVIPKHTIKTQPLEDTPSSAPAAPMVDSLIARVGAMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQRAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRIPTECIERIEAILKELEKAAL"
     gene            complement(1445..2611)
                     /gene="sopA"
                     /locus_tag="POB34_004771"
     CDS             complement(1445..2611)
                     /gene="sopA"
                     /locus_tag="POB34_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HDI5921591.1"
                     /translation="MRLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
BASE COUNT          821 a          711 c          626 g          735 t
ORIGIN      
        1 taatcagacc aacaatatga gtgggaccgt ggtcccagac tgattagcag accgacaata
       61 tgagtgggac cgtggtccca gactgattag cagaccaaca atatgagtgg gaccgtggtc
      121 ccagactgat tagcagaccg acaatatgag tgggaccgtg gtcccagact gattagcaga
      181 ccaacaatac gagtgggacc gcggtcccag actgattagc agactaacaa tatgagtggg
      241 cccgtggtcc cagaccgata atcagaccga caatatgagt gggaccgtgg tcccagactg
      301 attatcagac cgacaatatg agtgggaccg tggtcccaga ctgattatca gaccgacaat
      361 acaactggga ccgtggtccc agatataata ttcaggccag ttagttatgt tttctagcct
      421 gtgctaaagg aaattaagta aagacagata aatgtagact aaaacgtgcc gcatcaaagt
      481 gccgcttttt caagttcctt aagaatggct tcaattctct ctatacactc agttggaata
      541 cgagatctat ctaggtttaa caccatttta tcgcccttgt acaacactgt cgctccagga
      601 gcaaactgat gtcgtgagct taagctagtt cttgaggcag atgacgtttt aagcacagac
      661 gttaaaagag tgatgacttc ttcagcttca aatatcactc ctgctttttt ctgctcgtga
      721 aggttagatg cctgctgctt aagtaattct tctttatctg taaaagctct ttgaagtgca
      781 tcacctgacc gggcagatag ttcaccggga tgagaaaaaa gagcaacaac tgatttaggc
      841 agtttagctg tgttgataca gcgggtaata atcttacgtg aaatattttc tgcatcagcc
      901 agcgcagaaa tatttccagc aaattcattc tgcaatcggc ttgcataacg ctgaccacgt
      961 tcataagcac ttgttggcct ataatcgtta cccaatctgg acaatgcagc catctgctca
     1021 tcatccagct caccaaccag aactcgataa tcactttctg taagtgcagc agctttacga
     1081 cggcggctcc catcggcaat ttctatgacc ccagacactc ttcgaccgaa tgctggtgtc
     1141 tgctgacctg tcagtagaaa agaagggata agatcatcca gggcgtcttc ggtaagcagt
     1201 tcctggtcac gttcattacc tgaccatacc cgagaggtct tctcaacact atctccccgg
     1261 agcacctcaa gggtaaattt cacgtcccta ccacatacag gcaaagtaat agcattgcca
     1321 cgagccatag ctcctacgcg cgcaattaac gaatccacca tcggtgcagc tggtgccgat
     1381 gacggagtat cttcaagcgg ttgagtctta atcgtatgtt ttggaatgac aggcgcacgc
     1441 ttcattatct aatctcccag cgtggtttaa tcaaacgatc gaaaatttca ttgcagacag
     1501 gttcccaaat agaaagggca tttctccagg cacctgttga agagcgttga tcaatagcct
     1561 gttcaaaaac agttctcatc cggatctgac ctttaccaac ttcatccgtt tcacgcacaa
     1621 cattttttag gaccatgctt ccccaggcgt cccgaatttg ctcctccatc cacggggact
     1681 gagaaccatt actattactg tatttggtaa gcaaaatacg tacatcaggc tcgaatcctt
     1741 taagatctac gtttttgagc agatcacgaa gcatatcgaa aaactgcaga gcggaagtgt
     1801 agtcgaacaa ctcagcaggc gtggggacaa tcaacacatc agcagcacat acaacattaa
     1861 tcgtgccgat acctaggtta ggcgcgctgt caatgacaat gacatcatag tcatgagcga
     1921 cggtttcaat ggccagtcgg agcatcaggt gtggatcggt gggcaattta ccttcatcaa
     1981 atttgcccat tagctcagtt tcaatgcggt gcagagccaa acaggaagga ataatgtcaa
     2041 gcccaggcca gcaagtaggc tttattgcat aagtgacatc gtccttttcc ccaagataga
     2101 agggaaggag agtatcctct gcatgaatat gaagatctgg cacccagccg tgatacatcg
     2161 atgctgttcc ctgggggtcg ttaccttcca cgagcagaac acgtaatccc ttcagagcca
     2221 gatcctgagc aagatgaaca gaaacagagg ttttgtaaac gcccccttta tgagcagcaa
     2281 ctccaatcac cggcggaaat acgtcttcag cacgtcgtag tctcgtacca aacacgtcac
     2341 gcatatgatt aatttgttca attgtataac caacacgttg ctcaacccgt cctcgtattt
     2401 ccatatccgg gtgcggtagc cggcctgctt tctcggcatc cctgatagcc tgagaagata
     2461 ctccaactaa atccgctgct tcacctattc tccagcgtcg ggtgattttc ctcgcttccg
     2521 ggctgtcatc attaaactgt gcaatggcga tagccttcgt catttcatga ccagcgttta
     2581 tgcactggtt aagtgtttcc atgagtctca ttctgaacat cctttaatca ttgctttgca
     2641 tattttcatt taatcttgca ttttattgca aagcaacaac aaaattgcaa agtagtcaaa
     2701 aaaccgcaaa gttgtttaaa atgagagcaa cacatcaaaa aaggaaacag gaagagcaca
     2761 tacctcagtc aataattatc actagtactc gtagccgtcg tgtaaccgag cgtagcgagc
     2821 taactggcga ggacgcgcgc taactgttca cactcagtat ccctagcacc aaaataacta
     2881 tctgatggtc aag
//
LOCUS       DAMZET010000307         2880 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.307, whole genome shotgun sequence.
ACCESSION   DAMZET010000307 DAMZET010000000
VERSION     DAMZET010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.307"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1114)
                     /gene="phrB"
                     /locus_tag="POB34_004772"
     CDS             complement(<1..1114)
                     /gene="phrB"
                     /locus_tag="POB34_004772"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HDI5921592.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMISASFLVKDLLIDWREGERYFMSQL"
     gene            complement(1111..1620)
                     /locus_tag="POB34_004773"
     CDS             complement(1111..1620)
                     /locus_tag="POB34_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HDI5921593.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(2047..>2880)
                     /locus_tag="POB34_004774"
     CDS             complement(2047..>2880)
                     /locus_tag="POB34_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HDI5921594.1"
                     /translation="DKDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVIGQIKT
                     DEKSNEITAIPELLNMLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLN
                     KAFEEKFPLKELNNPAHDCSAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLC
                     VAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNE
                     DDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGL
                     S"
BASE COUNT          747 a          715 c          622 g          796 t
ORIGIN      
        1 tcagctgcga catgaaatat cgctcgcctt cgcgccagtc gatcaacaaa tctttcacca
       61 gaaaactggc tgaaatcatc cgtagccggt tatgcatcca gccagtgctg ttaagctgac
      121 gcatggcggc atcgacaatc gggtatcccg ttttgccttc ctgccaggcc tgtaaatgtg
      181 cgggattgct ctgccactgc acacgatccg tccaggcaat aaacggacaa tgtttacaca
      241 acgaggggta atacgtcatc agatgacggt agaactcgcg ccagatcagc tcactaagcc
      301 agacactacc ggccccaccg tccagcgcct gcggctgttc cgccaacaag cgatgcaaac
      361 actggcgagg cgataacccg cccgttgcca ggctggcgga caaacgactg gtgccttcca
      421 ctgccggaaa atctcgttgt tgctcatatt ctccggcacc gttctggcaa aactggcgta
      481 attgcgcaat cgccgctttt tcttccaccg ggaaatgcgc agtatcgaaa gactgacgag
      541 gataattcag cgtaatggat ggcgagggct ctatcgatcc gctactacga acttttggcg
      601 cagcgacgca ctccggcatc ccttcccgca gccgtttcag ccaggcattc ttaaaaggcg
      661 taaagacttt gtacatctcg tgattaccgg tcatcaccgc gccaggcggc aggatcacgc
      721 tgtcatcaaa tccttcacac accacgttac gcagcgctct ttcaacttcc acatcccgcg
      781 cccgctcatt cacttcatac tgatagttat aaaacaggtg ggtaacgctg ttttccgcgc
      841 acacctgttt aactatttcg acactggcga caaagtcatc cacttcacgg aacaataaag
      901 gaataccttt ttccgcaagc gctatttgta gcccattcag ttgagcattg ataagttcag
      961 cctgacgcgg cgacatgtta tgcgtcgccc actggcgtgg tgtagcgata tacaacgcca
     1021 gcacgcgtgc agacgaattg cggcaggcgg cagccagtgc gagattatcg tgcagacgta
     1081 aatcctggcg aaaccagacc agatgggtag tcataaaact cctgaatggc gcaagttaat
     1141 cacacgcggc caaagttcga aataacgctg ccctgaaagc caggcgtcag gataaagcgc
     1201 catatagtgt ttgatacgca tcagcggcgc aagaagtggt tgctcgccac ggcgataact
     1261 gtcgataagc gcagccagct gctggcgttc tgtggaatca atatgcgggc gaaaataacc
     1321 ctgaacgtgc atcaaaacat tggtgtgatc gcgcgggttg gcggggtgag aaagcaaaac
     1381 aactacgcgt tggcggtact ggttgtaata gtcatccaga ttttgccact ggtgaatatc
     1441 agccacgaac gggccaagtt tgcggtactc cggctgagaa tgcgccagaa agacgagttt
     1501 atagcggctg tgataatcga gaagcgcccc gcgcgttaag ccatgttctt tcagtcgatg
     1561 taactcatga agggcaaaga tacggattag ggtgctgtca tcaaatcgtt gtagattcat
     1621 tactgctcct gaacggtata aaggagtaag tgtagacgat ggtaaatgaa ttttatgggg
     1681 cagagttttt gcggggaagt attttgttgg taaaaatatt atcaacttca tctgagcgag
     1741 aattattcta ctatttcccg agctgcattc attttttgta ttttttattt ttagaaaata
     1801 acagttctca aagcttaatt acctcactat taattgtatt tcaaactaat aatcgcactc
     1861 cattttctct ttttaaaaaa cataaacact gttaaaacaa atacttattt cgttatctta
     1921 aatgctattt ctacatcact cgtccttata atcaaattat tcttattcat agctattttt
     1981 gcaggtttaa aatagcggct ttcaccaaag tcagccatga aaattagact acaagtcagg
     2041 gcaagattac gaaagcccgc tccccgcaag gactgacgcc agatagtttc tgtccatggc
     2101 tgcttttcgc atcttacgtc ttaaccctgc cttgaatacc ttatcattcg tcaaaatatt
     2161 aatagcaatg tgccgtatcc ctgaaaataa ttctgctgca tttcctcttc ttattttgca
     2221 gtcgtcttca ttcattacca cgtccagacg ccagtgcagc ttattctcca cgtgccagtg
     2281 gtttcggatt gctgtggcga acttttctgc agttaaatca gcagaactga tataatatct
     2341 gaccgtcatt tctggctctt tcttttgttc tgctattatg gaccgaaagg agactgccac
     2401 gcataatttc ttcagtcctt tccattcaaa cgtgaaatca ataagttcat cagggacatc
     2461 gcaaacaata tgaagacgga tttcttctct gccgtgactc ttttcactaa ttgcggaaca
     2521 gtcatgcgct ggattattta attctttcag cggaaatttt tcctcaaagg ctttattaag
     2581 ccgcccctgg tttcctttta cagcgaataa ataatcacct ccctgttttt gtatcttctc
     2641 tgcaatatct ttctggcaac ccatcgcatc agttgtgata atttttcctt taatatctaa
     2701 catgttaaga agttcaggga tagctgtaat ctcattagat ttctcatccg tcttgatctg
     2761 tccgatgacc agactgtgca ttgttgagaa cgcactaatg acatgaatcg ctcccttgcg
     2821 gcgactcttg tcataagagt gccggagcgt ttttccatca attgcgatga cgtctttatc
//
LOCUS       DAMZET010000308         2840 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.308, whole genome shotgun sequence.
ACCESSION   DAMZET010000308 DAMZET010000000
VERSION     DAMZET010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.308"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1009)
                     /locus_tag="POB34_004775"
     CDS             complement(<1..1009)
                     /locus_tag="POB34_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HDI5921595.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQ"
     gene            1122..2054
                     /locus_tag="POB34_004776"
     CDS             1122..2054
                     /locus_tag="POB34_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI5921596.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(2051..>2840)
                     /gene="purR"
                     /locus_tag="POB34_004777"
     CDS             complement(2051..>2840)
                     /gene="purR"
                     /locus_tag="POB34_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HDI5921597.1"
                     /translation="AVEKNCFQKGYTLILGNAWNNLEKQRAYLSMMAQKRVDGLLVMC
                     SEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVIDNAFEGGYMAGRYLIERGHREI
                     GVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQGDFEPESGYRAMQQILSQPHR
                     PTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDNVRNARYFTPALTTIHQPKDSL
                     GETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADGPFRDYRR"
BASE COUNT          708 a          743 c          779 g          610 t
ORIGIN      
        1 tctgcaacgc tgcggcgcga ccagttgcgt gtgggaaggg acgcagcgcc tgggcgacaa
       61 caatcgggta gatcgcgcca ttggcaatcg ccatcacaca gaatgggatc aggatttcga
      121 ccagcgacac atggctaatg aagcctgcag cccaggtcgc aatgacgctg actgcaaaca
      181 gcaccagcaa ccacggtaat aactgcttgc cttgccattt ctgcagcgcg gcgcgacagc
      241 cataaccacc aatcagaaac gcgatagttt gcgggacata acttaaacca ataactgccg
      301 ggctgtagcc catttcacta aggatgaacg gtgaaccggt cagccatgca aaaaaactgg
      361 ctgaacaggc cgcgtatatc agcacgttgc cgcgataggt tttagaacgt agcaggtcgg
      421 taaaggtcag accatcctga ctattgttac gggccttcgt cgtgggtttg agccagaaaa
      481 taggcagaat cagcaccacg gtaatggcaa acagggtggc gaaaatcgcc tgccaggaaa
      541 aatggaccag cagccagctt cctaacagag gagccagggc cggagataga cccaccagcg
      601 gcatgatggt cgcaaaaatt cggttaactt tctgtgaagg ataataatcc gtcactaacg
      661 cttgccagat aaccgccgcg gcgcagacac ccacagcctg tacaaaacgc aataccagca
      721 gcgtagcggc gttttctacc cacagcatcc ccagactacc taacgcaaaa attgtcaggc
      781 cgattaataa caccggttta cgaccataac ggtcggagag tggcccccac agaagctggg
      841 ctgcggcaaa accggcaagg aacagactaa ggctggcact gacagcagac gcaggcgttt
      901 gcaggtcggc ctgtatggcg gcgaaagcag gcagatacat atcggttgcc agaaaaccga
      961 gtacgctcaa acccgccagc cagactaaaa atcttttccc aggttgcatg tatattctct
     1021 aaatctctca attaaacacc gccgcaaagt gtagggagtg aaattagtgt tgtgaaacgg
     1081 taatatttga tgactggttt caaaaatttt gcaggcagac tatgtggtca gaatattcac
     1141 tcgaagttgt tgatgcggta gcgcgtaatg gtagttttag cgctgcggca caggagctgc
     1201 atcgcgttcc ttctgcggtc agctataccg tgcgtcagct ggaagagtgg ctggcggtgc
     1261 cgctctttga acgacggcac cgtgatgtgg agctgaccgc tgctggagcg tggtttctca
     1321 aagaagggcg ctctgttgtc aaaaaaatgc agatcacccg ccagcaatgt cagcagatag
     1381 cgaacggctg gcgcggtcag ttagctatcg cagtggataa tattgtcagg ccagaacgta
     1441 cacggcagat gatcgttgat ttttatcgcc attttgatga tgtcgaactt cttgtctttc
     1501 aggaagtgtt caacggtgtc tgggatgcgc tttccgacgg gcgcgtggaa ctggcgattg
     1561 gcgcaacacg cgcgattccg gtaggcggtc gttatgcctt ccgggatatg gggatgctaa
     1621 gctggagttg cgttgttgcc agccaccacc cgctggcgtt gatggatggc ccattcagcg
     1681 atgatacgtt gcgcaactgg ccgtcgttgg tgcgcgaaga cacctcgcga acgctaccta
     1741 aacgtattac ctggttgctg gataaccaaa aaagagttgt cgtgcctgac tgggaatcat
     1801 cggcaacctg tatctcggca ggattatgca tagggatggt gccaacacat ttcgccaaac
     1861 cgtggcttaa tgaggggaag tgggtagcac ttgaattgga aaaccccttc ccggattcgg
     1921 catgttgtct gacatggcag caaaatgata tgtcgcctgc gttaacctgg ctgcttgagt
     1981 atctcggcga tagtgagacg ctgaataagg agtggctgcg ggagccggaa gagactcccg
     2041 caacgggtga ttaacgacga tagtcgcgga acgggccgtc agccacggag cggcgttcaa
     2101 tcaagcgcgg atgcacttca atggactgcg gttcttcacg tttgttgacg atacgatcca
     2161 acagcatgtt gaacgctgtt tcacccagcg aatcttttgg ctggtggatc gtggtcagcg
     2221 ccggcgtaaa atagcgggcg ttgcgcacgt tatcataacc gatcagcgaa acatcctgcg
     2281 ggacgcgcag acccatttca tcagcagcac aaagtgcgcc cattgccatg atatcgccac
     2341 cacagaagac ggcagtaggg cgatgcggct gcgacagtat ttgctgcatg gcgcgataac
     2401 cagattcggg ttcaaagtca ccctgaacaa tccagctttc cggcaccttg atcatcgctt
     2461 cttccatcgc cttcataaaa ccggcaaggc ggcctgcgcc ggtgttacgt tccagcgggc
     2521 cggggatgac gccgatttcg cggtgaccgc gttcaatcag ataacgcccg gccatgtagc
     2581 cgccttcgaa cgcgttatca atgaccgcat cggtgaagtc agcttttgct tcaccccagt
     2641 ccatcaccac cattgggata tggcgatact cttccagcat cgccagcaac ggctctgggt
     2701 actcagaaca catcaccagc agaccatcga cgcgtttttg cgccatcatc gacagataag
     2761 cccgctgttt ctcaagattg ttccacgcat tacccagaat cagggtgtaa cctttctgga
     2821 agcaattttt ttcaaccgct
//
LOCUS       DAMZET010000309         2837 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.309, whole genome shotgun sequence.
ACCESSION   DAMZET010000309 DAMZET010000000
VERSION     DAMZET010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.309"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(246..464)
                     /gene="infA"
                     /locus_tag="POB34_004778"
     CDS             complement(246..464)
                     /gene="infA"
                     /locus_tag="POB34_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDI5921598.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(749..1453)
                     /gene="aat"
                     /locus_tag="POB34_004779"
     CDS             complement(749..1453)
                     /gene="aat"
                     /locus_tag="POB34_004779"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HDI5921599.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(1495..>2837)
                     /gene="cydC"
                     /locus_tag="POB34_004780"
     CDS             complement(1495..>2837)
                     /gene="cydC"
                     /locus_tag="POB34_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HDI5921600.1"
                     /translation="DTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDFTLAFTLGGIML
                     LTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGASDRYRTQLENT
                     EIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQPGALIALFVFCA
                     LAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVADRVSLTLRDVQ
                     FTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDPQQGEILLNDSP
                     IASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESLRRVGLEKLLED
                     AGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDATTESQILELLAE
                     MMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRYYQFKQGL"
BASE COUNT          752 a          735 c          716 g          634 t
ORIGIN      
        1 ttgctaccaa acctcatggg tggcaacggg gcgctactat agggagttgg agtaaaacgg
       61 tcaagaagaa ttttaatgat aattattgtt tgctcatact gtaaacaagt tgtgcagtat
      121 atctacatcg agacaagtta cggacttata cttccaaagt acttcataca tatcacaaaa
      181 taaaaaggcc ggttaaaccg accttttact cgttctttct cttcgcccat caggcggtaa
      241 aacaatcagc gactacggaa gacaatgcgg cctttgctca ggtcgtacgg ggtcagttca
      301 acagtcactt tgtcgcccgt caggatgcgg atgtagtttt tgcgcatttt accggagatg
      361 tgtgcagtaa ccacgtgacc gttttctaac tctacgcgga acatggtatt aggcaacgtt
      421 tcaagaacgg taccttgcat ttcaatattg tcttctttgg ccatctaatc ctctggggta
      481 tcactaccgt aatttgaacc ggcaagataa tgccgaagtt ctgtaaataa gtaaagattt
      541 gcgcgctaaa tcgcaacaaa caggttcggc acattactcc gaaaacacac ggctaagccg
      601 caccaaaagc gcaacgtata agggagcggt gagataaacg atgggcgtta cctgacgcga
      661 aaaaatcctt atcggcagcg gggtaatgag cgtaaccaac tctgcgaccg caattataac
      721 actctgggga gaaatgtgcc gaaaacattc attcttgtgg tgaaaacaag catcgtggta
      781 cccagaaatt attcggcaat cgtccgaggc gcatttgatt gagataatta aggtaatccc
      841 ggcggggaat ttcgcaggca ccaagcgatg ctgtgtgatc gttaaggacc tggcagtcga
      901 taagcttacc gccatgaccg ataaattcct cacagaatac cagaagcgcc gttttagacg
      961 cattttccat ccggctgaac atggactcac cacaaaatag cgttccctgg gccacgccgt
     1021 acataccgcc gacaagctca tcttcacgcc agacttcaat ggagtgggca tggccgagtt
     1081 cgtgaaggcg atggtaggct tcgaccacgc cacgcgtgat ccaggttcct tcttcgcgat
     1141 cgctggcaca gccttcaatg acctgaccaa aagcgtaatt catcgtgaca cgatagggcg
     1201 agcgtttatg aaatcgcttc atactacggc tgatatgcag tgattctggc catagcaccg
     1261 cgcggggatc gggcgaccac cagaggatgg ggtcgcctgg agaaaaccac ggaaaaatac
     1321 cacgctggta agccattaac aggcgcgcag ggctaagatc gcccccaagt gccagcaggc
     1381 cgttaggctc acgtaatgcg ccttccgggg aagggaaggc tattgaatgg cgagaaagct
     1441 gaaccaggcg catgaccgca aaactccacg caagtcggat cgttcaataa tagcttacaa
     1501 accctgcttg aactggtaat aacgcccctg tctggcaagc agctctgcgt gagtaccttg
     1561 ctcaataatt tgcccgttgt ccatcactat tatttgttgg aaacgagaga gtccgcgaag
     1621 tcgatgggtg accattaaca ccgttttctc acgcatcatt tctgcaagca attcaaggat
     1681 ctggctttcg gttgtggcat ctaagccttc ggtaggttca tccagcaaca ccagtggcgc
     1741 atcatgtaac agcgcacggg cgatagccag acggcgcagt tcaccaccgg agagctggcg
     1801 tccgccttca cctaaccaac tgttgagacc tgcatcctcg agcagcttct ccagaccaac
     1861 gcgacgcaag ctctccgcca gagcctcatc actactgcca ggcgaggcga gtaacagatt
     1921 atcacgcagc gtggcgctaa acagatgcac tcgctgagga acaacgctga tggtctgtcg
     1981 tagagccgct tcattcaggc tggctatggg gctatcgtta agcaaaatct ctccctgttg
     2041 cgggtcccaa gcgcgggtca gcagttgtaa cagtgttgat ttgccgcatc cggttcgccc
     2101 gagaatcgca atatgttccc cggcgtttac ctgaagagaa atccctttaa gtgcctgttg
     2161 ggattgctcc ggataagtga actgaacatc ccgtaatgtc agcgaaacgc gatcggcaac
     2221 acgagtttgg gtatcaggga aggtgacttc cggtttttga tccgttaaat cagtgatccg
     2281 aacggcagag gcaatgactt gccccagatg ctgaaatgca cccgttactg gtgccagtgc
     2341 ttcaaacgcg gctaacgcgc agaagacaaa cagggcaatt aacgcgccgg gttgagcatt
     2401 gccgccaacg ccgccagacg ccatccacag catcaggatc accgctaacg cgccaatgag
     2461 cagcattatc gcttgcgaca atgcggtcag ttcagattga cggcgttgcg cttccagcca
     2521 ttgaatttct gtattctcta gttgcgtgcg ataacgatcg ctggcgccaa aaatggtcag
     2581 ctcagcttgc ccttgcagcc aggccgtcag ttgttggcga tactgtccgc gaagatgagt
     2641 cagattttgc ccggtgcttt ttcccgcacg ataaaacagc ggtggcatca ggaaaagcgt
     2701 cagtaacata atgccgccca gcgtaaaggc gagggtgaaa tcaaggaaac ttaacccgat
     2761 tgtcaccacc ataatcacca caaaagcgcc caccagcggc gagataacgc gcagataaag
     2821 atgatcgagc gtatcaa
//
LOCUS       DAMZET010000310         2785 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.310, whole genome shotgun sequence.
ACCESSION   DAMZET010000310 DAMZET010000000
VERSION     DAMZET010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.310"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1282
                     /locus_tag="POB34_004781"
     CDS             <1..1282
                     /locus_tag="POB34_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HDI5921601.1"
                     /translation="LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKV
                     ILVGERESLADFQEMEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPA
                     PGADAWPVLIREAARYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQR
                     EWREGFLAERMQDEILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVH
                     IGDGEFTDIERKAELGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDG
                     DSASMAELCALISALADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQREL
                     TGKQGVIIPTANVRHLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTT
                     LMQTIQERIAQASQQEGRHRFPWPLRWLNWFIPN"
     gene            1351..1869
                     /gene="fabA"
                     /locus_tag="POB34_004782"
     CDS             1351..1869
                     /gene="fabA"
                     /locus_tag="POB34_004782"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HDI5921602.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(1939..2106)
                     /gene="rmf"
                     /locus_tag="POB34_004783"
     CDS             complement(1939..2106)
                     /gene="rmf"
                     /locus_tag="POB34_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HDI5921603.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(2362..>2785)
                     /gene="pqiC"
                     /locus_tag="POB34_004784"
     CDS             complement(2362..>2785)
                     /gene="pqiC"
                     /locus_tag="POB34_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HDI5921604.1"
                     /translation="VEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTL
                     VANLSTQLPGWVVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRP
                     FRLEGVQTQDGYDEMVKVLAGVWSQEAASIAQEIKRLP"
BASE COUNT          685 a          700 c          720 g          680 t
ORIGIN      
        1 actgctggcg caacctctgc tgtggatgcg gctgaaaaat atcgttaacc gcgagcgttt
       61 tgactgggtt gcgtttgatg agtcgcgccc tctccccgtc tctgtgcctt cgatgccatt
      121 gaagctgaaa gtcattctgg taggcgaacg cgaatcattg gctgatttcc aggagatgga
      181 gccagagctt tcagagcagg ctatttatag cgaatttgaa gatactctgc agattgtcga
      241 tgcggagtca gtaacccagt ggtgtcgctg ggtgacattt accgccagac ataatcactt
      301 acctgcaccg ggagcggatg cctggccggt acttatccgc gaagcagcac gctacaccgg
      361 tgaacaagaa acacttccgc ttagcccgca gtggatcctc cgccagtgta aagaggtcgc
      421 ctccctgtgt gatggcgaca ccttctccgg cgagcagcta aacttaatgc tgcagcagcg
      481 tgaatggcgc gaaggtttcc tcgctgaacg tatgcaggat gagatccttc aggagcaaat
      541 cctgattgaa accgaaggcg aacgcatcgg gcaaattaac gccctttcgg tcattgaatt
      601 tccgggtcat ccacgcgctt ttggcgaacc ttctcgcatt agctgcgttg tgcatattgg
      661 cgatggtgaa ttcaccgaca tcgaacgcaa agcggagctt ggcggcaata tccatgcgaa
      721 agggatgatg atcatgcaag cgttcctgat gtcggaacta cagcttgagc aacagatccc
      781 cttctcagca tcgctgacat ttgagcagtc atacagtgaa gttgatggag atagtgcctc
      841 gatggctgaa ctctgcgccc tgataagcgc cctcgccgat gtgccggtga atcagagtat
      901 cgctatcaca ggttcagtcg atcagttcgg tcgcgcccag ccggtcggtg gtttaaatga
      961 gaaaatcgaa ggcttctttg ctatttgcca gcaacgtgag ttaaccggga aacaaggtgt
     1021 cattatcccc acagctaacg ttcgccattt aagtcttcac agtgaactgg tgaaagcggt
     1081 agaagaaggc aaattcacca tctgggcagt agacgatgtg actgacgcac tgccgttatt
     1141 attaaatctg gtgtgggatg gcgaaggcca aacgacgctg atgcaaacca tccaggaacg
     1201 tatcgcgcaa gcatcgcaac aggaaggacg tcaccgtttt ccatggccat tacgttggct
     1261 gaactggttt attccgaact gatcggactt gttcagcgta cacgtgttag ctatcctgcg
     1321 tgcttcaata aaataaggct tacagagaac atggtagata aacgcgaatc ctatacaaaa
     1381 gaagaccttc ttgcctctgg tcgcggtgaa ctgtttggcg ctaaaggccc gcaattgcca
     1441 gcaccgaaca tgctgatgat ggaccgtgtg gtcaaaatga ccgaaacggg tggtaacttc
     1501 gacaaagggt atgttgaagc agaactggat atcaatccgg atctgtggtt cttcggatgc
     1561 cactttattg gcgatccggt tatgccggga tgcctgggcc tggacgcaat gtggcagctg
     1621 gtagggttct acctcggctg gctgggcggc gaaggtaaag gccgcgcgtt gggcgttggc
     1681 gaagtgaaat tcactggtca ggtactgccg acagcgaaaa aagtgaccta ccgtattcac
     1741 tttaaacgca ttgttaaccg tcgtctgatt atgggcctgg cggatggcga agtgctggtt
     1801 gatggtcgtc tgatctatac cgccagcgac ctgaaagtcg gtctgttcca ggatacgtct
     1861 gccttctgat tataatcaga cagagtatca aaaggcgaaa cctccgcaat gcggaggttt
     1921 ctttttaaag agacagaatc aggccattac taccctgtcc gccatggctt ctcgccagcc
     1981 tcccagccat tgtgaccttt gattcagcgt ctgataggga cacatttctt ttgagcgtcc
     2041 ggcgatgccg gcctgataac cacgttgatg tgcccgttcc aggcgatctc gtttttgtct
     2101 cttcatgcct cgtttccctc atactggttt ctggtggaaa agaaaacagt gattactaaa
     2161 tgtgcaatca cagttaagca ataccgcgaa tcataactgc cgtcaatgcg caaaattcac
     2221 gccactgtca tatttgtgag ctacacgggc atgtcggttg tacaaaaaat gtgaagagga
     2281 acaaattttt ccgcacgtta gagacaaaaa aaccgcctca ggcaacatac cggagacggt
     2341 tatatttaca aatctttata attaaggtag acgctttatc tcttgtgcaa tagaagcggc
     2401 ttcctgactc cagacaccgg ccagcacttt aaccatctca tcgtaaccat cctgagtttg
     2461 cactccttcc agacggaacg gacgtttgat cagttgtccc tggtggttca acagccactc
     2521 accactgaca atgaccttgc catcatagcg accgttaaac tccgttacgg taacattgag
     2581 cgtgtcctgg gcgcttccca gaggctggga ggcaaccacc cagccgggca gttgcgtact
     2641 caggttggca accagggtgt tgcgcaactg ttgatccaac gggctggccc acaagttgtt
     2701 gttggcaatc acatacttca catcactggt ttgataaacc acaccattcc ccgccagata
     2761 gtcaggaaca gtgacctgct ctacc
//
LOCUS       DAMZET010000311         2779 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.311, whole genome shotgun sequence.
ACCESSION   DAMZET010000311 DAMZET010000000
VERSION     DAMZET010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.311"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..703
                     /locus_tag="POB34_004785"
     CDS             <1..703
                     /locus_tag="POB34_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDI5921605.1"
                     /translation="QLNDVAGKMKIIEARLSALTNNQTKSLKLKPVALPKVASQLPDE
                     LGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQDALHLSDLFRHSA
                     QQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAVNDVARAASQVQTL
                     AEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRERANGLLLQGQWLD
                     ASIQLTGALGGGYKR"
     gene            756..1517
                     /locus_tag="POB34_004786"
     CDS             756..1517
                     /locus_tag="POB34_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI5921606.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(1647..2225)
                     /locus_tag="POB34_004787"
     CDS             complement(1647..2225)
                     /locus_tag="POB34_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDI5921607.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            2395..>2779
                     /locus_tag="POB34_004788"
     CDS             2395..>2779
                     /locus_tag="POB34_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1282 family protein"
                     /protein_id="HDI5921608.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPL"
BASE COUNT          661 a          716 c          783 g          619 t
ORIGIN      
        1 gcagctcaac gatgtcgcgg ggaaaatgaa aattattgag gcacggttaa gcgcacttac
       61 aaataaccag acaaagtcat tgaagcttaa accggtcgcg ttgccgaaag tggcaagcca
      121 gcttcctgat gaactggggt actccttact ggcccggcgg gcagatttgc aggcggcgca
      181 ctggtacgtt gagtcatcgc taagcaccat tgatgcggca aaagcggcat tttatcctga
      241 catcaacctg atggccttcc tgcaacagga tgcgttgcac ttaagcgatc tgttccgtca
      301 ttccgcgcag caaatgggcg ttacggcagg cctgacgcta cccattttcg atagtggtcg
      361 tcttaacgcc aatctcgata tcgcaaaagc cgaaagcaac ttgtctatcg ccagctacaa
      421 caaagcggtg gttgaagcgg tgaatgacgt ggcgcgggca gccagtcagg ttcagacact
      481 tgcggagaaa aaccagcatc aggcgcaaat tgagcgcgat gccttgcgtg tggtaggtct
      541 tgcgcaggcg cgctttaacg cgggcatcat tgctggttcc cgcgtcagcg aagccagaat
      601 ccccgcgctg cgtgagcggg ccaatggcct gttattgcaa gggcagtggc tggatgcttc
      661 cattcaactc actggtgcgt tgggcggggg ttacaaacgc tgatgatatg cgccttctat
      721 acttaacgtt tattcagcgt taagtggaga actcgatggc acaggttgcg attattaccg
      781 cctccgattc ggggatcggc aaagagtgcg cgttattact ggcgcagcag gggtttgata
      841 ttggcattac ctggcactca gatgaagaag gggcaaaaga taccgcgcgt gaggtagtta
      901 gccacggcgt acgtgcggag atcgtgcagc tggatctcgg taaactgcca gaaggtgcgc
      961 aggcgctgga gaaactcatt caacggctgg ggcgtattga tgtgctggtg aataatgcgg
     1021 gcgcaatgac caaagcgccg tttcttgata tggctttcga tgagtggcgc aagattttta
     1081 ccgttgatgt cgatggtgca tttttatgct cacaaattgc ggcccgccag atggtgaaac
     1141 aagggcaggg cggtcgcatc atcaacatta cgtcggtaca tgaacatacg ccgctgccgg
     1201 atgccagcgc ttacacagcc gctaaacatg cgctcggcgg gttaaccaaa acgatggcgc
     1261 tggagctggt caggcataag attttggtga acgcagtcgc gcctggggcg atcgccacgc
     1321 caatgaatgg catggatgac agcgacgtga agcccgacgc ggagccttcg attcctctgc
     1381 ggcgttttgg cgcaacgcat gagattgcca gcctggtagc gtggctgtgc tcagagggcg
     1441 caaattacac caccgggcag tcgttgatag tggatggcgg ctttatgttg gcgaatccgc
     1501 agttcaaccc agaatagcgc agtgaggtgc gtggttattt caatgggtaa aattttggta
     1561 ggcatgataa gacgcacaag cgtcgcatca ggcatggtgc accggatgtc ggatgcggcg
     1621 cgaacgcctt acccagggca atggttttac gcctgatgat ccggcttttt cttcccgcgc
     1681 cgtttcagcc accagcgcac gcccaccacc agaaccacga ccagaatcaa ccagacccag
     1741 tgcttcaaat gctggtcgag attgtgcaac cacggcgcaa tcacctgacc accagcgtaa
     1801 ccaatagtgg taaaaatcaa cgcccaggca aatgcgccga gaatattcag cggcagaaag
     1861 attttcggcg gcagctggct ggcaccaatc agcgtcgggc caatcacccg aaagccatac
     1921 ataaagcgcg taccaatgac aaacagatac ggatggcgtt ggataagttt ctgcgcccgc
     1981 tcaattttat cctgatgttt cgagaaacgg cgtaacagct tgccgccaaa ccgccgcccg
     2041 cacagataga gcacctggtc gccaatcatg ccgccaagcg ccacagaaag taccaccagc
     2101 gggaacttta atagcccctg atgcgccgca acgcctccca gcaaagtcac ggtttcacct
     2161 tccgccaggc taccgatcac cagcgcggca taaccatatt gtgagataag tgtattgaga
     2221 tccataggcc agtctgtctc cctttggttg ccagtaagca tacaccctgt gaatcattca
     2281 cgctgaaaaa ggacgttcat cagacagtga aaattacatg ggcgaacaaa aaataatcac
     2341 aagctgactt atacttgaac tgtcaggttc atgaactcac aacaaggggg cggtatgagc
     2401 catgtctggg gactgttttc ccatcccgat cgtgaaatgc aggtgattaa tcgcgaaaac
     2461 gaaacgattt ctcaccatta cacccaccac gttttgctga tggcggcgat tccggtgatt
     2521 tgcgccttca ttggcactac acagattggc tggaattttg gcgatggcac tatcctgaag
     2581 ttatcctggt ttaccggact ggcgctggct gtcttgtttt acggcgttat gctggctggg
     2641 gtcgcggtga tggggcgggt catctggtgg atggcgcgta attatccgca gcgtccgtca
     2701 cttgcgcact gcatggtctt cgcgggctac gttgcaactc cgctgttttt aagcggtctg
     2761 gtggcgcttt atccactgg
//
LOCUS       DAMZET010000312         2778 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.312, whole genome shotgun sequence.
ACCESSION   DAMZET010000312 DAMZET010000000
VERSION     DAMZET010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.312"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..254
                     /locus_tag="POB34_004789"
     CDS             <1..254
                     /locus_tag="POB34_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HDI5921609.1"
                     /translation="NPATLENESDKQKLMILLRTFFEVHKGWHIQYNIVSRETLLEAK
                     KHPDQYRDLVVRVAGYSAFFTALSPDAQDDIIARTEHML"
     gene            400..1215
                     /gene="ybiV"
                     /locus_tag="POB34_004790"
     CDS             400..1215
                     /gene="ybiV"
                     /locus_tag="POB34_004790"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HDI5921610.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPHFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            1367..2632
                     /locus_tag="POB34_004791"
     CDS             1367..2632
                     /locus_tag="POB34_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HDI5921611.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
BASE COUNT          699 a          707 c          700 g          672 t
ORIGIN      
        1 tgaatccggc aacgctggag aacgaatctg acaagcagaa actgatgatc ctgctgcgca
       61 ccttctttga agtgcataaa ggctggcata ttcagtacaa catcgtttcc cgcgaaacgc
      121 tgctggaggc gaaaaaacat ccggatcagt accgcgatct ggtagtgcgt gtcgcgggct
      181 attccgcctt cttcaccgcg ctctctccag acgcacagga cgatatcatc gcccgtactg
      241 aacatatgct gtaatctcct cggcccggcg gcgtcgtcgc cgggctaaat cattcacatc
      301 atcaatttca tccttacttt cattcgaaat ataatttgtg ctctgcgtca cattgttatt
      361 acactgttac ggtttcattt tcgagccagg agcacaccta tgagcgtaaa agttatcgtc
      421 acagacatgg acggtacttt tcttaacgac gccaaaacgt acaaccaacc acattttatg
      481 gcgcaatatc aggaactgaa aaagcgcggc attaagttcg ttgtcgccag cggtaatcag
      541 tattaccagc ttatttcgtt ctttcctgag ctaaaggatg agatctcttt tgtcgcggaa
      601 aacggcgcac tggtttacga acatggcaag caactgttcc acggcgaact gacccgacat
      661 gaatcgcgga ttgttattgg cgagttgcta aaagataagc aactcaattt tgtcgcctgc
      721 ggtctgcaaa gtgcatatgt cagcgaaaat gcccccgaag catttgtcgc actgatggca
      781 aaacactacc atcgcctgaa agctgtaaaa gattatcagg agattgacga cgtactgttc
      841 aagttttcgc tcaacctgcc agacgaacaa atcccgttag tgatcgacaa actgcacata
      901 gcgctcgatg gcattatgaa acccgtcacc agtggttttg gctttatcga cctgattatt
      961 cccggtctac ataaagcaaa cggtatttcg cggttactga aacgctggga tctgtcaccg
     1021 caaaatgtgg tagcgattgg cgacagcggt aacgatgcgg agatgctgaa aatggcgcgt
     1081 tattcctttg cgatgggcaa tgctgcggaa aacattaaac aaatcgcccg ttacgctacc
     1141 gatgataata atcatgaagg cgcgctgaat gtgattcagg cggtgctgga taacacatcc
     1201 ccttttaaca gctgacctct ctaccggagc cgtgcgctcc ggttttccct tctttttaca
     1261 tcctgtacat cacatcaaat cattaaactt gcattaactt atttataact tatattgtca
     1321 catgcaaatc agaatggttt cgaatgaaag ttaatgagag gtggttatgg cttctacttt
     1381 taccagcgac acattgcctg ccgatcacaa agcagctatc cgtcagatga agcacgcgct
     1441 gcgggcgcag cttggcgacg tccagcagat ctttaatcag ctaagcgatg acattgccac
     1501 gcgagtggct gaaatcaacg cactcaaagc acagggcgat gccgtctggc cggtgctgtc
     1561 ttatgccgat atcaaagcag gtcatgttac tgcagagcag cgcgaacaga ttaaacgtcg
     1621 cggttgtgcg gtgataaaag gccatttccc ccgcgaacaa gcgctaggct gggatcagtc
     1681 gatgctggac tatctggacc gcaaccgctt tgacgaggtc tacaaaggcc ccggcgataa
     1741 tttcttcggg acgctcagcg cttcacgtcc cgagatttac cccatctact ggtcgcaggc
     1801 gcaaatgcag gcccgccaga gtgaagaaat ggcgaatgcg cagtcgtttc tcaatcgtct
     1861 gtggacattt gaaagtgatg gaaagcaatg gtttaacccg gatgtgagcg tcatctaccc
     1921 tgaccgtatc cgccgccgtc cgcccggaac gacctccaaa ggtcttggag cgcataccga
     1981 ctccggggcg ctggaacgct ggctgcttcc agcgtatcag cgcgttttcg ccaacgtctt
     2041 taatggcaat ctggcgcaat atgatccctg gcatgcggca catcgtacgg aagttgaaga
     2101 gtacacggtg gacaacacca ccaaatgttc cgtgtttcgg acattccagg gctggacagc
     2161 gctctctgat atgctgcctg gtcaggggct gctgcacgtt gtgcccattc ctgaagccat
     2221 ggcgtacgta ctgttacgtc cgctgcttga tgatgtgccg gaggatgaac tgtgcggcgt
     2281 agcgcccgga agagtgttgc cggtatcaga gcaatggcat ccactgttaa ttgaggcgtt
     2341 aaccagcatt ccaaaactcg aggccggaga ctccgtctgg tggcactgcg acgtcatcca
     2401 ttccgttgcc cccgttgaaa atcaacaggg ttggggcaac gtgatgtaca ttcctgcggc
     2461 accgatgtgc gagaaaaatc ttgcctacgc gcacaaggtg aaggccgcac tggaaaaagg
     2521 cgcatcgccg ggcgacttcc cgcgcgagga ctatgaaaca aactgggaag gacgctttac
     2581 gcttgccgac ctcaacattc acggtaagcg agcgttgggc atggatgttt gagagttaat
     2641 ggaagtgaat tgatgtggga ttaagaaaat cgattcgacg ttactcagat tgctgacaag
     2701 tgcgctttct taatgcctga tgcggcgtta acgccttatc tggcctacaa agtcctgcag
     2761 attcaatata ttacaggt
//
LOCUS       DAMZET010000313         2760 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.313, whole genome shotgun sequence.
ACCESSION   DAMZET010000313 DAMZET010000000
VERSION     DAMZET010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.313"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            202..1320
                     /locus_tag="POB34_004792"
     CDS             202..1320
                     /locus_tag="POB34_004792"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDI5921612.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAATDHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDILRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1386..1634
                     /locus_tag="POB34_004793"
     CDS             1386..1634
                     /locus_tag="POB34_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HDI5921613.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVSTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYLVLRFIWRRWFSLAD"
     gene            1699..2067
                     /locus_tag="POB34_004794"
     CDS             1699..2067
                     /locus_tag="POB34_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDI5921614.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2161..>2760
                     /gene="nfsB"
                     /locus_tag="POB34_004795"
     CDS             2161..>2760
                     /gene="nfsB"
                     /locus_tag="POB34_004795"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HDI5921615.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAADNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFN"
BASE COUNT          676 a          694 c          673 g          717 t
ORIGIN      
        1 ttttctgcat gactcaatcg tcaaaattct tcgccaccag tcagcactgc cataacttgt
       61 cacattgcaa aagtagccaa attgcttcag gcaccatcag ttcaccatca ccaaaaaaaa
      121 gaaacattcc agaacttccg catctcgccc agccgctccg acttctatac tgaatagaaa
      181 acgccaacat aagagaaacc tatgccatta cccgattttc atgtttctga accttttacc
      241 ctcggtattg aactggaaat gcaggtggtt aatccgccgg gctatgactt aagccaggac
      301 tcttcaatgc tgattgacgc ggttaaaaat aagatcacgg ccggagaggt aaagcacgat
      361 atcaccgaaa gtatgctgga gctggcgacg gatgtttgcc gtgatatcaa ccaggctgcc
      421 gggcagtttt cagcgatgca gaaagtcgta ttgcaggcag ccacagacca tcatctggaa
      481 atttgcggcg gtggcacgca cccgtttcag aaatggcagc gtcaggaggt atgcgataac
      541 gaacgctatc aacgcacgct ggaaaacttt ggttatctca ttcagcaggc gaccgttttt
      601 ggtcagcatg tccatgttgg ctgcgccagt ggcgatgacg ccatttattt gctgcacggc
      661 ttgtcacgat ttgtgccgca ctttatcgcc ctttccgccg cgtcgccata tatgcaggga
      721 acggatacgc gttttgcctc ctcacgaccg aatatttttt ccgcctttcc tgataatggc
      781 ccgatgccgt gggtcagtaa ctggcaacaa tttgaagccc tatttcgctg tttgagttac
      841 accacgatga tcgacagcat taaagatctg cactgggata ttcgccccag tcctcatttt
      901 ggcacggtgg aggttcgggt gatggatacc ccgttaaccc ttagccacgc ggtaaatatg
      961 gcgggattaa ttcaggccac cgcccactgg ttactgacgg aacgcccgtt caaacatcag
     1021 gagaaagatt acctgctgta taaattcaac cgtttccagg cctgtcgcta tgggctggaa
     1081 ggcgtcatta ccgatccgca cactggtgat cgtcgaccgc taacggaaga tatcttgcga
     1141 ttgctggaaa aaatcgcccc ttccgcacat aaaattggcg catcgagcgc aattgaagca
     1201 ctgcatcgcc aggtcgtcag cggtctgaat gaagcgcagc tgatgcgcga tttcgtcgcc
     1261 gatggcggct cgctgattgg gctggtgaaa aagcattgtg agatctgggc cggtgactaa
     1321 gtcgggaatt gccttacaga agaatatccc tgacaatggt gttttaactt acatttaaca
     1381 atgttatgaa acacccttta gaaaccttga ccaccgcagc aggcattttg ctgatggctt
     1441 tcctctcttg cctgctgctt cccgcccccg cattggggct gacgctggca caaaaactgg
     1501 tgagcacgtt ccatctgatg gatcttagtc agctttacac tttattgttt tgtctgtggt
     1561 ttttagtgct gggcgctatt gagtatcttg ttctgcgctt tatctggcga cgctggttct
     1621 cgctggcgga ttaaacgtcg attatcgccg tacttcgcgg catactttgc ttattctcct
     1681 tcgcctaaag gaacgtttat ggataagcaa tcactgcacg aaacggcgaa acgcctggcc
     1741 cttgagttac cctttgtcga gctttgctgg ccttttggcc cggagttcga tgtttttaaa
     1801 attggcggca agatttttat gctgtcgtcg gagctacgcg gcgtcccctt tatcaatctg
     1861 aagtccgatc cacaaaaatc cctgttaaat cagcaaatat acccaagcat taagccaggg
     1921 tatcacatga ataaaaagca ctggatttcg gtgtatcccg gcgaggaaat ctccgaagcg
     1981 ttacttcgcg atctgatcaa cgattcgtgg aatctggtgg ttgatggtct ggctaaacgc
     2041 gatcaaaaaa gagtgcgtcc aggctaaagc ggaaatctat agcgcatttt tctcgcttac
     2101 catttctcgt tgaaccttgt aatctgctgg cacgcaaaat tactttcaca tggagtcttt
     2161 atggatatca tttctgtcgc cttaaagcgt cattccacta aggcatttga tgccagtaaa
     2221 aaacttaccc cggaacaggc cgagcagatc aaaactctcc tgcaatacag cccatccagc
     2281 accaactccc agccgtggca ttttattgtt gccagcacgg aagaaggtaa agcgcgtgtt
     2341 gccaaatccg ctgccgataa ttatgtgttc aacgaacgta aaatacttga tgcctcgcac
     2401 gtcgtggtgt tctgtgcaaa aaccgcgatg gacgatgcct ggctgaagct ggttgttgac
     2461 caggaagatg ctgatggccg ctttgccacg ccggaagcga aagccgcgaa cgataaaggt
     2521 cgcaagttct tcgctgatat gcaccgtaaa gatctgcatg atgatgcaga gtggatggca
     2581 aaacaggttt atctcaacgt cggtaacttc ctgctcggcg tggcggcgct gggtctggac
     2641 gcggtaccca tcgaaggttt tgacgccgcc atcctcgatg cagaatttgg tctgaaagag
     2701 aaaggctaca ccagtctggt ggttgttccg gtaggtcatc acagcgttga agattttaac
//
LOCUS       DAMZET010000314         2715 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.314, whole genome shotgun sequence.
ACCESSION   DAMZET010000314 DAMZET010000000
VERSION     DAMZET010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.314"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            190..1659
                     /gene="gspA"
                     /locus_tag="POB34_004796"
     CDS             190..1659
                     /gene="gspA"
                     /locus_tag="POB34_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HDI5921616.1"
                     /translation="MSTRREVILSWLCEKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSIGRLTEH
                     ELKASILEGQNIDQPDLLLTARVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIALTCGWLLLSSFTATLPVPAWLIPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSGNASLQTL
                     VDQNLPWIASLKVGDKKLPVVVVRVGEASVDVLVGQQTWTLTHKWFESVWTGDYLLLW
                     KMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPLLVEKIKQFQKSHHLK
                     TDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            1661..2080
                     /gene="gspB"
                     /locus_tag="POB34_004797"
     CDS             1661..2080
                     /gene="gspB"
                     /locus_tag="POB34_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HDI5921617.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWVLWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPGVA
                     VENAPSSSEDEENNVEESDEKAGLRERVKNALNELER"
     gene            2318..2629
                     /gene="rpsJ"
                     /locus_tag="POB34_004798"
     CDS             2318..2629
                     /gene="rpsJ"
                     /locus_tag="POB34_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDI5921618.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
BASE COUNT          740 a          574 c          704 g          697 t
ORIGIN      
        1 gtgtgggcac gatgtatgtt ctaataaaat agattgggtg ggttgtatat tgacgttagt
       61 ataaatagta ttaatgccgc ttgcatgaat caagcaaaat ttatttatat tagtaatatg
      121 tatttaatta ataaatacat acattatttt tatgattaag gataatcaat aattaaaaca
      181 ggaagttcta tgtctacgag aagagaagtt attctttcct ggttgtgtga gaaacgtcaa
      241 acctggcgtc tatgctattt gttgggtgag gctggaagtg gaaaaacctg gctggcgcag
      301 caactgcaaa aagataaaca tcgccgtgtg attactttaa gcctcgttgt ttcctggcaa
      361 ggtaaggccg catggatcgt taccgacgat aacgcggctg aacagggctg ccgtgacagc
      421 gcctggacgc gagatgagat ggcggggcaa ttactgcatg cgcttcatcg aactgatagt
      481 cgctgccctc ttattattat tgaaaatgct cacctgaatc accgccgcat acttgatgat
      541 ctccaacgcg caatatctct tattcctgac ggtcaatttt tgctgatcgg cagacccgat
      601 cgaaaagtcg aacgtgattt taaaaaacag ggcattgaac ttgtctcgat aggacgtctg
      661 acggagcacg aacttaaggc aagcatcctt gaaggtcaga atattgacca acccgatctc
      721 cttttaaccg ccagagttct gaaacggata gctttattat gtcggggcga tcgcagaaag
      781 ctggcgcttg ccggcgaaac aattcgctta ttacagcaag ccgagcagac cagcgttttc
      841 acagcaaaac aatggcgaat gatttaccgc atattgggtg ataaccgccc ccggaaaatg
      901 cagcttgccg tggtgatgag tggaaccatt atcgctctta cctgtggttg gctactgctg
      961 tcctctttta cagctacgtt acccgttcct gcgtggctga tacccgtaac gccagtagtt
     1021 aaacaagata tgactaagga tattgctcat gtggtgatgc gcgatagtga ggcgctaagc
     1081 gtgttatacg gcgtatgggg gtacgaggtc ccagctgatt cggcctggtg cgatcaggca
     1141 gtaagagcgg ggctggcatg taaaagtggg aatgcttctt tacaaacatt ggtagatcag
     1201 aatttgccct ggatcgcatc gcttaaagtc ggggacaaaa aattgcctgt tgtggtcgtt
     1261 cgcgtcggtg aggctagcgt tgatgtcctc gtaggtcagc aaacgtggac gctgacacat
     1321 aaatggtttg agtcggtatg gacgggtgat tatcttctgt tgtggaaaat gtcgccggaa
     1381 ggggagagta ccattacgcg tgatagcagc gaggaagaga ttctctggct ggaaacgatg
     1441 ttaaaccgcg cattgcatat ttcgacagaa ccttcggcgg agtggcgtcc cttgttggta
     1501 gaaaaaataa agcaatttca aaaaagccac cacttgaaaa ctgacggtgt tgttggcttt
     1561 tcaacgttag tacatctctg gcaggtggca ggggagagtg cctacttata tcgggatgaa
     1621 gcaaatattt ccccggaaac aacagtgaag gggaaatgac atgtttgaat tctatatcgc
     1681 cgcgcgtgag cagaaagaaa caggtcaccc tggtatcttc tcccgacaaa aacacagcac
     1741 cataatatat gtaatttgtt tattactgat ttgcctgtgg tttgcaggaa tggtgctggt
     1801 tggtgggtat gccaggcagc tatgggtgct ttggatagta aaagccgaag tcactgtaga
     1861 ggctgaaacg cccgctttta aacaatcaac acaacactat ttcttcaaaa aacagccact
     1921 ccccgtcgtc gaatccgtcg aggaggaaga tgacccaggc gtcgcggttg agaatgcccc
     1981 ctcttcttct gaagacgaag aaaataatgt ggaagaaagc gacgagaaag ccggtttaag
     2041 agagcgtgtc aaaaatgcac tgaacgaact tgagagataa cccgaaggct gtttacttta
     2101 ctagcaatac gcttgcgttc ggtggttaag tatgtataat gcgcgggctt gtcgtagttg
     2161 acagcaggtt caatctgaac ctcagttagc cgaatttggc tacctaacaa tgctcccaat
     2221 cggggagcta cgtaagaacg gttacactct cccatcaatc gtaatgggtc tgaggagtaa
     2281 tcattttcgt ttataaaata attggagctc tggtctcatg cagaaccaaa gaatccgtat
     2341 ccgcctgaaa gcgtttgatc atcgtctgat cgatcaagca accgcggaaa tcgtcgagac
     2401 tgccaagcgc actggtgcgc aggtccgtgg tccgatcccg ctgccgacac gcaaagagcg
     2461 cttcactgtt ctgatctccc cgcacgtcaa caaagacgcg cgcgatcagt acgaaatccg
     2521 tactcacttg cgtctggttg acatcgttga gccaaccgag aaaaccgttg atgctctgat
     2581 gcgtctggat ctggctgccg gtgtagacgt gcagatcagc ctgggttaat caggtcattg
     2641 agcgattgag aggttgaaac aatgattggt ttagtcggta aaaaagtggg tatgacccgt
     2701 atcttcacag aagac
//
LOCUS       DAMZET010000315         2683 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.315, whole genome shotgun sequence.
ACCESSION   DAMZET010000315 DAMZET010000000
VERSION     DAMZET010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.315"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1058
                     /locus_tag="POB34_004799"
     CDS             <1..1058
                     /locus_tag="POB34_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HDI5921619.1"
                     /translation="QSEGGKGVEPLMKMSWNYKQPHEPQSDEVAKENNGYALEDLYDA
                     NGVLIAKKGQLLSSFAHLRDDGTTASSCWIYTGSWTEQGNQMANRDNSDPSGLGNTLG
                     WAWAWPLNRRVLYNRASADINGKPWDPKRMLIQWNGSKWTGNDIPDFGNAAPGTPTGP
                     FIMQPEGMGRLFAINKMAEGPFPEHYEPIETPLGTNPLHPNVVSNPVVRLYEQDALRM
                     GKKEQFPYVGTTYRLTEHFHTWTKHALLNAIAQPEQFVEISETLAAAKGINNGDRVTV
                     SSKRGFIRAVAVVTRRLKPLNVNGQQVETVGIPIHWGFEGVARKGYIANTLTPNVGDA
                     NSQTPEYKAFLVNIEKA"
     gene            1071..1955
                     /gene="fdnH"
                     /locus_tag="POB34_004800"
     CDS             1071..1955
                     /gene="fdnH"
                     /locus_tag="POB34_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HDI5921620.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            1948..2601
                     /gene="fdnI"
                     /locus_tag="POB34_004801"
     CDS             1948..2601
                     /gene="fdnI"
                     /locus_tag="POB34_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HDI5921621.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
BASE COUNT          646 a          649 c          788 g          600 t
ORIGIN      
        1 accagtccga aggtggtaaa ggcgtagaac cgctgatgaa gatgagctgg aactacaagc
       61 agccgcacga accgcaatct gacgaagtgg ctaaagagaa caacggctac gcgctggaag
      121 atctctatga cgctaatggc gtgcttattg cgaagaaagg tcagttgctg agtagctttg
      181 cgcatctgcg tgatgacggt acaaccgcat cttcttgctg gatctacacc ggtagctgga
      241 cagagcaggg caaccagatg gctaaccgcg ataactccga cccgtccggt ctggggaata
      301 cgctgggatg ggcctgggcg tggccgctca accgtcgcgt gctgtacaac cgtgcttcgg
      361 cggatatcaa cggtaaaccg tgggatccga aacggatgct gatccagtgg aacggcagca
      421 agtggacggg taacgatatt cctgacttcg gcaatgccgc accgggtacg ccaaccgggc
      481 cgtttatcat gcagccggaa gggatgggac gcctgtttgc catcaacaaa atggcggaag
      541 gtccgttccc ggaacactac gagccgattg aaacgccgct gggcactaac ccgctgcatc
      601 cgaacgtggt gtctaacccg gttgttcgtc tgtatgaaca agacgcgctg cggatgggta
      661 aaaaagagca gttcccgtat gtgggtacga cctatcgtct gaccgagcac ttccacacct
      721 ggaccaagca cgcattgctc aacgcaattg ctcagccgga acagtttgtg gaaatcagcg
      781 aaacgctggc ggcggcgaaa ggcattaata atggcgatcg tgtcactgtc tccagcaagc
      841 gtggctttat ccgcgcggtg gctgtggtaa cgcgtcgtct gaaaccgctg aatgtaaatg
      901 gtcagcaggt tgaaacggtg ggtattccaa tccactgggg ctttgagggt gtcgcgcgta
      961 aaggttatat cgctaacact ctgacgccga atgtcggtga tgcaaactcg caaacgccgg
     1021 aatataaagc gttcttagtc aacatcgaga aggcgtaagg gggcgaacag atggctatgg
     1081 aaacgcagga cattatcaaa aggtccgcaa ctaactccat cacgccgcct tctcaggtgc
     1141 gtgattacaa agcagaagtc gcaaaactta tcgacgtttc cacctgtatc ggctgtaaag
     1201 cctgtcaggt ggcgtgttcg gagtggaacg acatccgtga tgaagtgggg cactgcgtcg
     1261 gggtttacga taaccccgcc gatctgagcg ccaagtcctg gacggtgatg cgctttagcg
     1321 aaaccgaaca gaacggcaag ctggagtggc tgatccgtaa agacggctgt atgcactgtg
     1381 aagatcccgg ctgcctgaag gcgtgcccgt ctgctggtgc aatcattcag tacgctaacg
     1441 ggattgtcga tttccagtcg gaaaactgca tcggctgtgg ttactgcatt gccgggtgtc
     1501 cgtttaatat tccgcgcctc aacaaagagg ataaccgggt atataaatgc acgctctgcg
     1561 tcgatcgcgt cagcgtcggc caggaaccgg cttgtgtgaa aacctgtccg accggggcta
     1621 tccacttcgg caccaagaag gagatgctgg agctggcgga acagcgcgtg gcgaaactga
     1681 aagcgcgtgg ttacgaacat gctggcgtct acaacccgga aggggtcggt ggtacgcacg
     1741 ttatgtacgt gctgcatcac gccgatcagc cggagctgta tcacggtctg ccgaaagatc
     1801 cgaagatcga cacctcggta agcctgtgga aaggcgcgtt gaaaccgctg gcagcggctg
     1861 gctttattgc cacttttgcc gggttgattt tccactacat cggtattggc ccgaataagg
     1921 aagtggacga tgacgaggag gatcatcatg agtaagtcga aaatgattgt gcgcaccaaa
     1981 tttattgatc gcgcctgtca ctggaccgtg gtgatttgct tcttcctggt ggcgctgtcc
     2041 gggatttcgt tcttcttccc gacgctgcaa tggctgacgc aaaccttcgg tacgccgcag
     2101 atgggacgca ttttgcaccc gttcttcggc attgcgattt tcgtcgcact gatgtttatg
     2161 tttgtgcgtt ttgtgcatca caacatcccg gataagaaag atattccgtg gctgttgaac
     2221 attgtcgaag tattgaaagg caatgagcat aaagtggcgg atgtcggtaa gtacaacgcc
     2281 gggcaaaaga tgatgttctg gtcgatcatg agcatgattt tcgtgctgct ggtgaccggg
     2341 gtgattatct ggcgtccgta ctttgcgcag tacttcccga tgcaggttgt tcgctacagc
     2401 ctgctgatcc acgcggctgc gggtatcatc ctgatccacg ccatcctgat ccatatgtat
     2461 atggcatttt gggtgaaagg gtcgattaaa gggatgatcg aagggaaggt gagccgtcgc
     2521 tgggcgaaga aacaccatcc acgctggtat cgtgaaatcg agaaggcaga agcgaaaaaa
     2581 gagagtgaag aagggatata atctcttttg aactttaagc tgaaaatggc gctgtaaaag
     2641 gcgccatttt catattgtag acaacgtagg ctttgttcat ccc
//
LOCUS       DAMZET010000316         2680 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.316, whole genome shotgun sequence.
ACCESSION   DAMZET010000316 DAMZET010000000
VERSION     DAMZET010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.316"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..496
                     /gene="msrQ"
                     /locus_tag="POB34_004802"
     CDS             <1..496
                     /gene="msrQ"
                     /locus_tag="POB34_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HDI5921622.1"
                     /translation="GRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLH
                     LTSYALLELGVNNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQ
                     LHNFVYLVAILAPIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHN
                     KLSV"
     gene            753..1403
                     /gene="zinT"
                     /locus_tag="POB34_004803"
     CDS             753..1403
                     /gene="zinT"
                     /locus_tag="POB34_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HDI5921623.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRKLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            1746..2276
                     /locus_tag="POB34_004804"
     CDS             1746..2276
                     /locus_tag="POB34_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HDI5921624.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
BASE COUNT          791 a          578 c          520 g          791 t
ORIGIN      
        1 cggtcgcact gcactgaaat ttttgctggc gaccttgtta atcacacctc tggcacgcta
       61 cgcaaaacag ccgttattga tacgcactcg ccgcctgtta ggcttatggt gctttgcctg
      121 ggcgacactg catttaacca gttacgcatt gctggagctg ggcgtgaata atctggcgtt
      181 attaggcaag gagttaatta cccggcctta tttaacgtta ggaattatca gctgggttat
      241 tttgcttgct ttagcgttta cttcaaccca ggcgatgcag cgaaaactcg gcaagcactg
      301 gcaacagttg cataatttcg tctatcttgt cgcgatcctg gccccaatac attatctgtg
      361 gtcagtgaag attatttcac cgcagccgct catctacgct gggctggctg tactgctttt
      421 agccttacgg tataagaagt tgcgttccct ttttaaccgg ttacgcaagc aggttcacaa
      481 caaattatct gtataacttc atattgccga caaagtacgc gttatccctg ccggatgcgg
      541 cgcgagcgcc ctatccggcc tacgcagatc acatcaggtt atgtttcgtt tgtcatcaac
      601 ctcaaattat caatataatt cattttattt caataagata gataatcaca cctgagagaa
      661 ctattctctt gtctcctaag agttgttgca ttttgtcata tgttacaata taacattaca
      721 tatcatatac aatgactctg gaggaaactg ttttggcgat tcgtcttcac aaactggctg
      781 ttgctttagg tgtctttatt gttagcgctc ctgctttttc gcatggtcat cactcacacg
      841 gcaaaccctt aacagaggtc gaacaaaaag ctgctaatgg tgtttttgat gatgccaatg
      901 tacaaaaccg aaagctcagt gactgggatg gagtctggca atccgtttat cctttactgc
      961 aaagtggcaa acttgaccct gtatttcaga agaaagcgga tgcagataaa actaaaacat
     1021 ttgctgaaat taaagattat tatcacaaag gttatgcaac agatatcgag atgattggca
     1081 ttgaggacgg cattgttgaa ttccatagaa ataatgaaac aacatcctgt aaatatgatt
     1141 acgatggata caaaatactc acctataaat caggcaagaa aggcgttcgt tacttattcg
     1201 aatgtaaaga tcctgaaagc aaagccccta aatatataca atttagtgat catataattg
     1261 caccacgaaa atcatctcat tttcacattt ttatgggtaa tgattcacag caatctttat
     1321 taaatgaaat ggaaaactgg ccaacgtatt atccatatca gttgagtagc gaagaagtgg
     1381 tcgaggaaat gatgtctcat tgagtattct catgataacg cctcgatgcc gctttagtaa
     1441 gtcatcataa ctgccactgg tccatccaca aacgccactg aacgcaagct agctacagac
     1501 acgctcatca ctatgacgtg tctgtatatt aataagctaa cccgcattga gttaaccaat
     1561 aacggatttc atacacaata cggccaacag agaaagatac ctgtgctcac gccattgctt
     1621 atattggctg gttacaatgt gcactatcaa ttttttaaat aacaaattct taaaaccaca
     1681 tctgtataag gattttttaa ggttcgtggg atagcttgac tgtaaaaatt acaggagcta
     1741 caaaaatgaa ccgattctca aaaactcaaa tttatttaca ttggataacg ctgcttttcg
     1801 ttgcaataac ctatgccgcg atggaactcc gtggctggtt tcctaaaggt agtagtactt
     1861 atctgctgat gcgagaaaca cattacaatg cgggtatatt cgtttgggtg ttaatgtttt
     1921 cacgcctgat tataaaacac cgttatagtg atccttctat tgtgccaccg ccacctgcct
     1981 ggcaaatgaa agcggcttcg ctaatgcaca tcatgcttta tataaccttc cttgcacttc
     2041 ctctgctggg gattgctttg atggcttaca gtggaaaatc gtggagtttc cttggtttca
     2101 atgtgtctcc ctttgttacc ccaaacagcg aaattaaagc actgataaaa aatattcacg
     2161 aaacctgggc aaatataggt tactttttaa tcgcagctca cgctggcgca gcactctttc
     2221 atcactacat tcagaaagat aatactctgt tacgaatgat gcctcgccgc aaataagtcg
     2281 atcccctgac aaggaaatgt gaaccatgga catcaacgcg tcataaatgt ttacgcaagt
     2341 gcacaatgca ccctttccac tatcaacaag gaggagcaga aactgtgtaa atcgtacaat
     2401 ggtaagactt atcaaaaata caggttaatt ttggtattta aacctttttt acttgttagt
     2461 atgatacaaa ggctttcaaa aaagctggga aattcaacaa ctcttggaaa ttatggactt
     2521 tgtcccgcgg ctttaatctg acggccgcgt tccttttttg actcaattat tgaccccttc
     2581 tctacgcaac ttcagttccc accaccaact ttgcggcagt ttcataggat gaatgtctaa
     2641 aagaataatg gtgagcgata agaaaacgac tgaataactg
//
LOCUS       DAMZET010000317         2675 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.317, whole genome shotgun sequence.
ACCESSION   DAMZET010000317 DAMZET010000000
VERSION     DAMZET010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.317"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..465
                     /locus_tag="POB34_004805"
     CDS             <1..465
                     /locus_tag="POB34_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDI5921625.1"
                     /translation="HLHQSGKVRHFGVSNFTPAQFALLQSRLPFTLATNQVEISPVHQ
                     PLLLDGTLDQLQQLRVRPMAWSCLGGGRLFNDDYFQPLRDELAVVAEELNAGSIEQVV
                     YAWVLRLPSQPLPIIGSGKIERVRAAVEAETLKMTRQQWFRIRKAALGYDVP"
     gene            546..1067
                     /gene="sodC"
                     /locus_tag="POB34_004806"
     CDS             546..1067
                     /gene="sodC"
                     /locus_tag="POB34_004806"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HDI5921626.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(1102..>2675)
                     /locus_tag="POB34_004807"
     CDS             complement(1102..>2675)
                     /locus_tag="POB34_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001700723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDI5921627.1"
                     /translation="IAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMMMILPSSSD
                     ATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQILTMNLLRIQAFWSHYR
                     FRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTALASSQTDVY
                     TVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDHTRLPRTSG
                     LARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLYSAVAAPFK
                     SLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQMPKFAALW
                     GQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFAILRLGSDARKSRRHI
                     RALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARRWLLRWGVVLLNCSHV
                     VWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRICDSLARHHQ
                     PAARELAAVLLAFAT"
BASE COUNT          699 a          679 c          714 g          583 t
ORIGIN      
        1 catctgcatc agagcggcaa agtgcgtcat tttggcgtat cgaactttac gcctgcgcaa
       61 tttgccctgt tgcaatcacg tctgccgttt acccttgcca ctaatcaggt ggaaatatcc
      121 ccggtgcatc agccgttact gctggatggc acgctcgacc aactacaaca actgcgtgtt
      181 cgtccgatgg cgtggtcctg ccttggtggt ggtcgtctgt ttaatgatga ttatttccag
      241 ccgctgcgtg atgaactggc tgtggtggca gaggagttaa acgcgggctc gattgaacag
      301 gtggtttacg cctgggtatt acgtttacca tcgcagccgc tgccaattat cggttcaggt
      361 aaaattgagc gcgtacgggc agctgtcgaa gcagaaacac tgaaaatgac ccgtcaacaa
      421 tggtttcgta tccgtaaagc ggcactgggg tacgacgtac cgtaagcact tttaggaata
      481 gccgccgttc aaaaatgtgt cactggttta cacttattca ggaatgcaca atgaacggag
      541 gtcctatgaa acgttttagt ctggctattc tggcgctggt tgttgcaacc ggcgcacaag
      601 ctgccagtga aaaagtcgag atgaacctcg tcacgtcgca aggggtaggg cagtcaattg
      661 gtagcgtcac cattactgaa accgataaag gtctggagtt ttcgcccgat ctgaaagcat
      721 taccccccgg tgaacatggc ttccatattc atgccaaagg aagctgccag ccagccacca
      781 aagatggcaa agccagcgcc gcggaatccg caggcgggca tcttgatcca caaaataccg
      841 gtaaacatga agggccagaa ggtgccgggc atttaggcga tctgcctgca ctggtcgtca
      901 ataatgacgg caaagctacc gatgccgtca tcgcgcctcg tctgaaatca ctggatgaaa
      961 tcaaagacaa agcgctgatg gtccacgttg gcggcgataa tatgtccgat caacctaaac
     1021 cgctgggcgg tggcggtgaa cgctatgcct gtggtgtaat taagtaatta agaggccagc
     1081 gtaccttgcg gtggtgcttg ctcaagttgc gaaagcgagc agtacagccg ccagctcacg
     1141 ggcggcaggt tgatgatgac gggcaaggct gtcgcaaatc cgctgtaatt cttcaagtgt
     1201 ggccgccagt gatttttgct gaacgccacg ctcactcatc actccccgca acagtgaaat
     1261 acagttatcc cgtactcgcg ataacggatc ggaacgcgat tcccagtcgc gcaattgcca
     1321 gacaacatga gaacagttca gcagcactac accccagcgt aataaccaac ggcgagccag
     1381 cgcatcctgg ctgttactca actgactgac gtgatgataa gtgagcgatt caaattcgct
     1441 ttcactcagt gttggatggc ggcttagctg atcgacaaaa tcccggcgca gcgcgcgaat
     1501 atggcggcgg cttttacgag catccgatcc tagacgcaga atggcgaacg ctaaccacgc
     1561 caacgcgacg ccaacgattt ttgccagatt atcgttaaga aaatcagcaa aatcatacac
     1621 cgggggatta gtgacagcga taaaagaacc cataaaaaca atcagttgcc cccacaatgc
     1681 ggcaaatttt ggcatctgca atttaagaag ctgcattgtc gccagcagtg gaaagagaaa
     1741 cagtaaaaat tgccacagat cgctaatctg gaccatcaga ccaaatttga ccacaaagct
     1801 gaatagcgaa agtaacacca gcgtgcgcat cagaagtgac aacgacttaa acggtgctgc
     1861 gacggcggag tagagtacgc agctaattgc tgccagcgtt aatgcattgg caccggcatc
     1921 ccattgcgaa gcaatactcc atgcgccaat catcatcaac gtacaaaatg tacgcagccc
     1981 gctccacata gcttcggcgt tatcggtatg acgagccagg ccggatgtcc gtgggagtct
     2041 ggtatgatca tctacaccgc tttgcagacg atgtaattcc tgactacttt gcagataaag
     2101 gcggcaaaaa taacgtagtc gctgccagaa ggcgacgtgc cgatagtcgg cgacgttggt
     2161 cgggcgtagc ggggcgataa tacgtgcgac ggtgtaaaca tctgtttgcg aactggcgag
     2221 cgccgtcagc aactgttcga gaatttctcg tgtggcacct ggcggtgagg gccagttgag
     2281 caacatacgt cgcaggctag agatgacact ggtcatacgt aattgctggt ggagcagcgc
     2341 attaaggcgc gcgttttgct ggcgaaaacg atagtggctc cagaaagcct ggatacgcag
     2401 caaattcatg gtcagtatct gcccaatcac cccttcatgt gctgcacgaa tggcatcggt
     2461 tgtttcaggc tgccagagta aactggcatg ttcaagtagt cgggcgtgca tgtttttcaa
     2521 tgcggttaaa agggctgtag catcggaact gctaggcagg atcatcatca tcatgccgcc
     2581 gcacaaaatg ccgacaatca cctcgcaaac gcgcgcctga gcgatatccc acagctggct
     2641 ggcctcagta atattaacca tcggaaaggc gatga
//
LOCUS       DAMZET010000318         2628 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.318, whole genome shotgun sequence.
ACCESSION   DAMZET010000318 DAMZET010000000
VERSION     DAMZET010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.318"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(1..131)
                     /locus_tag="POB34_004808"
                     /pseudo
     CDS             complement(1..131)
                     /locus_tag="POB34_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            complement(134..550)
                     /locus_tag="POB34_004809"
                     /pseudo
     CDS             complement(134..550)
                     /locus_tag="POB34_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
     gene            complement(1115..1297)
                     /locus_tag="POB34_004810"
     CDS             complement(1115..1297)
                     /locus_tag="POB34_004810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921628.1"
                     /translation="MLAVFQLVKNTCSRLLQCLQHAAAMRLAFANHLSFLYQSLRELI
                     MEYQVNADVIGFGYAA"
     gene            complement(1504..1830)
                     /locus_tag="POB34_004811"
     CDS             complement(1504..1830)
                     /locus_tag="POB34_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HDI5921629.1"
                     /translation="MKRRLLLLFLLSVLAVGCSQQKADEPRQLVTVYPRYPEYAAANY
                     IKGLVEVKFDIGADGTVTRIVFLRSEPHNLFRDEVVKAMAKWRFEKNRPCQGVKRQFI
                     FTPSRP"
     gene            2311..2604
                     /gene="iss"
                     /locus_tag="POB34_004812"
     CDS             2311..2604
                     /gene="iss"
                     /locus_tag="POB34_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HDI5921630.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITFGIYTPLEARVYCSK"
BASE COUNT          711 a          681 c          561 g          675 t
ORIGIN      
        1 cggatgacgc aggcattatg ctcgcagaga taaaacacgc tggaggggtc atccggcgtc
       61 catttgaggc caaacggcgt ctctttgtcg ccaaatttaa gatactgctc ctccccgcag
      121 tgcgggcagg caaacgccgc tgcaccgaca gggggatccc gtcgagaata ctggcaattt
      181 cacctgcgat ccgcgacagc acgaaagtac agaatgcggt ttccaccact tcagcggagt
      241 ctctggcatt tttcagctcc tgtgcgtcgg cctgcgcacg cgtaagtcga tggcgttcgt
      301 actcaatagt ccctggctgg agatctgtct cgctggcctg ccgcagttct tcaacttccc
      361 ggcgcagctt ttcgttctca atttcagcat ccctttcggc ataccatttt atgacggcgg
      421 cagagtcata aagcacctca ttacccttgc caccgcctcg cagaacgggc attccctgtt
      481 cctgccagtt ctgaatggta cggatactcg caccgaaaat gtcagccagc tgctttttgt
      541 tgacttccat tgttcattcc acggacaaaa acagagaaag gaaacgacag aggccaaaaa
      601 gctcgctttc agcacctgtc gtttcctttc ttttcagagg gtattttaaa taaaaacatt
      661 aagttatgac gaagaagaac ggaaacgcct taaaccggaa aattttcata aatagcgaaa
      721 acccgcgagg tcgccgcccc gtagcctgtt ggatcgccgg aaaggacccg aaaaacgaga
      781 ataattatca cttacagcaa gaatcgaatc tgatctatta tggtgcttgc tattatgtgc
      841 cggcacaagt gcgtcgttta ccgtcatttc acacagaggc atcatcaaat gaaaatcaga
      901 aatattctcg ctatctccct tgcaacatca tccttcagtt gcctggcatt taaatcctcg
      961 cccaatgtgc taccaggacc aacgaatcaa ctaactgcgg tagaaagtaa aattatcgga
     1021 catttttatg ccccacacag tgcattaccc ggaacaacca tcacagggac atgtgacgcc
     1081 tcccccgtcc cgggatgcac ctgtccgttt tgtactatgc tgcgtagcca aaaccgataa
     1141 catccgcatt tacctggtat tccatgatga gttcacgcag cgactgatag aggaaggaaa
     1201 gatggttagc aaaagcaagg cgcattgccg ccgcatgctg caggcattgc aacagacgag
     1261 agcaggtatt tttgaccagt tggaaaactg ccagcatact ttgcccgagt atatcgccat
     1321 ctcatcggaa accagtgcaa ctcttattca tcgggttcca ctagagaaaa agaagaaatg
     1381 aacagtgagg cgttgtgtgg catacaacgc cttcttccat cattcctcgt cagccatgac
     1441 aaaaatataa ccgctggctc tttcattttt ctcctgcttc cagcccctct ctacctggaa
     1501 gcatcaggga cgtgacggcg taaagataaa ttgtctcttc actccctgac agggacgatt
     1561 cttttcaaat cgccatttcg ccatggcctt caccacttca tcacgaaaca aattatgagg
     1621 ctctgagcgg agaaaaacga tccgtgtcac agtcccatca gcaccaatat cgaacttaac
     1681 ctcaaccagc cccttgatat aatttgctgc agcatattcc ggatatcgtg gatacaccgt
     1741 cactaattgc cggggctcat cagctttttg ctgcgagcat cccactgcta ggacagataa
     1801 cagaaaaagt agtaaaaggc gtcttttcat ttttattcct acgggtctta ttctgacaat
     1861 atatcctgtg ttccagactg ccacatcacc acatcctgtg ccattatctg actcacatta
     1921 catacatcgc atcgggatac agtagtagca ctttctgtaa tacagcttcc ggtttcttcc
     1981 accatcgcac cgggataaat ccgcgaatca ttaacgcggt aaaaacccgg tgtgcatcgt
     2041 ttttaattat tcccgcacac tcacgcagaa ggaattcccc gtcgggctac ggtcatggtt
     2101 aatgcgggaa tacggcgacg atacagcgca gctaaaaggg taatggacgg atagaccggt
     2161 ttatttcatt ctacaggatt ctgagtgtcc ccaactccct ccaatcgtct gagctcacct
     2221 gtgtagtttt aattttcatc aatccattta actatcgttt aattgttgtc acataggatt
     2281 ctgccgtttt taacaatgca ggataataag atgaaaaaaa tgttgttttc tgccgctctg
     2341 gcaatgctta ttacaggatg tgctcaacag acgtttactg ttggaaacaa accgacagca
     2401 gtaacaccaa aggaaaccat cacccatcat ttcttcgttt cgggaattgg gcagaagaaa
     2461 actgtcgatg cagcaaaaat ttgtggtggt gcagaaaatg tggttaaaac agaaacccag
     2521 caaacattcg taaatggatt gctcggtttc atcacttttg gcatttatac tccgctggaa
     2581 gcgcgggtgt attgctcaaa ataattgcat gagttgccca tcgatatg
//
LOCUS       DAMZET010000319         2520 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.319, whole genome shotgun sequence.
ACCESSION   DAMZET010000319 DAMZET010000000
VERSION     DAMZET010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.319"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..683)
                     /locus_tag="POB34_004813"
     CDS             complement(<1..683)
                     /locus_tag="POB34_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5921631.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMATALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTL"
     gene            790..1683
                     /locus_tag="POB34_004814"
     CDS             790..1683
                     /locus_tag="POB34_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI5921632.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            1818..>2520
                     /gene="mlc"
                     /locus_tag="POB34_004815"
                     /gene_synonym="dgsA"
     CDS             1818..>2520
                     /gene="mlc"
                     /locus_tag="POB34_004815"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HDI5921633.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHL"
BASE COUNT          640 a          607 c          673 g          600 t
ORIGIN      
        1 aataacgtct tagggcgcag cgaagtcggg cgaaaatggc gtgattccgg aaggattttc
       61 cagaacatca acgccgaggc cagcgcgaaa caaccgattg ccgccagagc aattcgccag
      121 ttgaaaaagt ccgtgaagac accactaatt aagcgtccgc tcatgccgcc aattgagttg
      181 ccgctgatat acaaccccat cgaaaaggcc acgaaactgg ggtggatttc ctcgctaaga
      241 taagtcatgc caacagctgc cacgccactt aacgaaagcc caatcaaggc gcgcataatc
      301 aaaatgccgt gccagctggt catcattgtc gaaagtaacg tacaaatgga ggccaacagt
      361 agcgccgtgg ccatcactgg tttgcgacca atggcatcgg atagggggcc agtaaacagc
      421 aaaccaatag ccaacatcgc cgtggaaatg gacagtgaaa tactactgtt cgcgggcgtt
      481 aagccaaact cctgcgaaag caccggaagg ataggctgca cacaatagag aagtgcaaat
      541 gttgccagtc cggcagagaa cagcgccagg gtgacgcgca taaattgcgg cgtaccgcgt
      601 ttaataaatt gatttggctg agaaatgctt tgcttgtcag tgtcgcttgc cggagcgcca
      661 tcaacagttg tagtacggct cacttgaaat ccttgctaaa tatgcctgtg gatcaggctt
      721 atacataggt taggaaaatc gaattgttct gtctaatata ttaataatct caaataagat
      781 gttttaaata tgaatattga acttcgtcat ctgcgttact ttgttgctgt tgcggaagag
      841 ctgcatttcg ggcgcgccgc tgcccgcctg aatatttcgc aaccaccgct aagtcagcag
      901 attcaggcac tggagcaaca aattggtgcc cgactgctgg cacgaactaa tcgcagtgta
      961 ttgctgacgg cagcaggaaa acagtttctt gcagatagtc ggcaaatcct gtctatggta
     1021 gatgacgctg ccgctcgcgc tgaaagactg catcaggggg aagcggggga gttgcgcatt
     1081 ggttttactt cgtccgctcc ttttattcgg gcggtgtccg atacgttatc gctgtttcgc
     1141 cgtgattatc ccgatgtcca tttacaaacc cgcgaaatga acactcgtga gcaaatcgct
     1201 ccgctcattg aaggaacgct ggatatggga ttgctgcgta acacagcgtt accggagacg
     1261 cttgaacacg cagtcatcgt ccatgaaccg cttatggcga tgatcccgca cgatcatccc
     1321 ctggcaaata acccgaatgt aacgctggct gaactggcga aagaaccctt cgtctttttt
     1381 gatccgcacg tcgggacagg gctgtatgac gatattctcg ggctgatgcg acgttaccat
     1441 ttgacgcccg tcatcactca ggaggtgggc gaggcaatga ccatcatcgg tctggtttcc
     1501 gccggtctgg gtgtttcaat tttgcctgct tcatttaaac gtgttcagct caacgaaatg
     1561 cgctgggtgc cgattgcaga agaggatgcg gtttctgaaa tgtggttggt ctggccgaaa
     1621 catcatgaac aaagtccagc tgcgcgtaac tttcgtattc atctgctgaa tgctctcagg
     1681 tgagggaaat ttcagcgaaa aagcccgaaa aatgtgctgt taatcacatg cctaagtaaa
     1741 aatttgacga cacgtattga agtgcttcac catagcctac agattatttc ggagcgcgaa
     1801 aatataggga gtatgcggtg gttgctgaaa accagcctgg gcacattgat caaataaagc
     1861 agaccaacgc gggcgcggtt tatcgcctga ttgatcagct tggtccagtc tcgcgtatcg
     1921 atctttcccg tctggcgcaa ctggctcctg ccagtatcac taaaattgtc cgtgagatgc
     1981 tcgaagcaca cctggtgcaa gagctggaaa tcaaagaagc ggggaaccgt ggccgtccgg
     2041 cggtggggct ggtggttgaa actgaagcct ggcactatct ttctctgcgc attagtcgcg
     2101 gggagatttt ccttgctctg cgcgatctga gcagcaaact ggtggtggaa gagtcgcagg
     2161 aactggcgtt aaaagatgac tcgccattgc tggatcgtat catttcccat atcgatcagt
     2221 tttttatccg ccaccagaaa aaacttgagc gtctaacttc gattgccata accctgccgg
     2281 gaattattga tacggaaaat ggcattgtac atcgcatgcc gttctacgag gatgtaaaag
     2341 agatgccgct cggcgaggct ctggagcagc ataccggcgt tccggtttat attcagcatg
     2401 atatcagcgc atggacgatg gcagaggcct tgtttggtgc ctcacgcggg gcgcgcgatg
     2461 tgattcaggt ggttatcgat cacaacgtgg gggcgggcgt cattaccgat ggtcatctgc
//
LOCUS       DAMZET010000320         2501 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.320, whole genome shotgun sequence.
ACCESSION   DAMZET010000320 DAMZET010000000
VERSION     DAMZET010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.320"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..581
                     /gene="cpsG"
                     /locus_tag="POB34_004816"
                     /gene_synonym="manB"
     CDS             <1..581
                     /gene="cpsG"
                     /locus_tag="POB34_004816"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HDI5921634.1"
                     /translation="YIVGLLAEAFLEKNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSK
                     TGHAFIKERMRKEDAIYGGEMSAHHYFRDFAYCDSGMIPWLLVAELVCLKEKTLGELV
                     RDRMVAFPASGEINSKLAQPAEAINRVEQHFSREALAVDRTDGISMTFADWRFNLRSS
                     NTEPVVRLNVESRGDVPLMEARTRTLLTLLNE"
     gene            636..2030
                     /gene="wcaJ"
                     /locus_tag="POB34_004817"
     CDS             636..2030
                     /gene="wcaJ"
                     /locus_tag="POB34_004817"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HDI5921635.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            2032..>2501
                     /gene="wzxC"
                     /locus_tag="POB34_004818"
     CDS             2032..>2501
                     /gene="wzxC"
                     /locus_tag="POB34_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HDI5921636.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFT"
BASE COUNT          559 a          622 c          743 g          577 t
ORIGIN      
        1 attacattgt cggcctgctg gcagaagcat tcctcgaaaa aaatcccggc gcgaagatca
       61 tccacgatcc acgtctctcc tggaacaccg ttgatgtggt gactgccgca ggtggcacgc
      121 cggtaatgtc gaaaaccgga cacgccttta ttaaagaacg tatgcgcaag gaggacgcca
      181 tctacggtgg cgaaatgagc gctcaccatt acttccgtga tttcgcttac tgcgacagcg
      241 gcatgatccc gtggctactg gtagccgaac tggtgtgcct gaaagagaaa acgctgggcg
      301 aactggtacg cgaccggatg gtggcgtttc cggcaagcgg tgagatcaac agcaaactgg
      361 cgcaacccgc tgaggcgatt aaccgcgtgg aacagcattt tagccgtgag gcgctggcgg
      421 tggatcgtac cgatggcatc agtatgacct ttgccgactg gcgttttaac ctgcgctcct
      481 ccaacaccga accggtggtg cggttgaatg tggaatcgcg cggtgatgtg ccgctgatgg
      541 aagcgcgaac gcgaactctg ctgacgttgc tgaacgagta atgtcggatc ttcccttacc
      601 ccactgcggg taaggggcta ataacaggaa caacgatgac aaatctaaaa aagcgcgagc
      661 gagcgaaaac caatgcatcg ttaatctcta tggtgcaacg cttttcagat atcaccatca
      721 tgtttgccgg actatggctg gtttgcgaag tgagcggcct gtcattcctc tacatgcacc
      781 tgttggtggc actgattacg ctggtggtgt tccagatgct gggcggcatc accgattttt
      841 atcgctcatg gcgcggtgtt cgggcagcga cagaatttgc tctgttgcta caaaactgga
      901 cgttaagcgt gattttcagc gccggactgg tggcgttcaa caatgatttc gacacgcaac
      961 tgaaaatctg gctggcgtgg tatgggctga ccagcatcgg actggtggtt tgccgttcat
     1021 gtattcgtat tggggcgggc tggctgcgta atcatggcta taacaagcgc atggtcgccg
     1081 tagcggggga tttagccgcc ggacaaatgc tgatggagag cttccgtaat cagccgtggt
     1141 tagggtttga agtggtgggg gtttaccacg acccgaaacc gggcggcgtt tctaacgact
     1201 gggcgggtaa cctgcaacag ctggtcgagg atgctaaagc gggtaagatt cataacgtct
     1261 atatcgcgat gcaaatgtgc gacggcgcgc gagtgaaaaa actggtccat caactggcgg
     1321 acaccacctg ttcggtgctg ctgatccccg atgtctttac cttcaacatt ctccattcac
     1381 gtctcgaaga gatgaacggc gttccggtgg tgccgctgta tgacacgccg ctttccgggg
     1441 ttaaccgcct gctaaaacgt gcggaagaca ttgtgctggc gacgctgatc ttgctgctga
     1501 tctccccggt gctgtgctgt attgcgctgg cggtgaaact cagttcacct gggccggtta
     1561 ttttccgcca gactcgctat ggcatggatg gcaagccgat caaagtgtgg aagttccgtt
     1621 ccatgaaagt gatggagaac gacaaagtgg tgactcaggc gacgcagaac gatccgcgcg
     1681 tcaccaaagt ggggaacttt ctgcgccgca cctcgctgga tgaactgccg cagtttatca
     1741 atgtgctgac cggggggatg tcgattgtcg gtccacgtcc gcacgcagta gcgcataacg
     1801 aacagtatcg ccagctcatt gaaggctaca tgctgcgcca caaagtgaaa ccgggcatta
     1861 ccggctgggc gcagattaat ggctggcgcg gcgaaaccga cacgctggag aaaatggaaa
     1921 aacgcgtcga gttcgacctt gagtacatcc gcgaatggag cgtctggttc gatatcaaaa
     1981 tcgttttcct gacggtgttc aaaggtttcg ttaacaaagc ggcatattga tatgagctta
     2041 cgtgaaaaaa ccatcagcgg cgcgaagtgg tcggcgattg ccacggtgat catcatcggc
     2101 ctcgggctgg tgcagatgac cgtgctggcg cggattatcg acaaccacca gttcggcctg
     2161 ctgactgtgt cgctggtgat tatcgcgctg gcagatacgc tttctgactt cggtatcgct
     2221 aactcgatta ttcagcgaaa agaaatcagt caccttgaac tcaccacgtt gtactggctg
     2281 aacgtcgggc tggggcttgt ggtgtgcgtg gcggtgtttt tgttgagtga tctcattggt
     2341 gacgtgctga ataacccgga cctggcaccg ttgattaaaa cattgtcgct ggcgtttgtg
     2401 gtgatccccc acgggcaaca gttccgcgcg ttgatgcaga aggagctcga gttcaacaaa
     2461 atcggcatga tcgaaaccag cgcggtgctg gcgggcttta c
//
LOCUS       DAMZET010000321         2383 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.321, whole genome shotgun sequence.
ACCESSION   DAMZET010000321 DAMZET010000000
VERSION     DAMZET010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.321"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..442
                     /locus_tag="POB34_004819"
     CDS             <1..442
                     /locus_tag="POB34_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HDI5921637.1"
                     /translation="LAYRKNLLYVHSLRELTVSAVLPGIRSLLADLRAQQIPVGLASV
                     SLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGLGVPPQACIGIEDAQA
                     GIDAINASGMRSVGIGAGLTGAQLLLPSTESLTWPRLSAFWQNV"
     gene            456..1538
                     /locus_tag="POB34_004820"
     CDS             456..1538
                     /locus_tag="POB34_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5921638.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEAKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETGIALR"
     gene            1583..>2383
                     /gene="ompG"
                     /locus_tag="POB34_004821"
     CDS             1583..>2383
                     /gene="ompG"
                     /locus_tag="POB34_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HDI5921639.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQN"
BASE COUNT          600 a          553 c          674 g          556 t
ORIGIN      
        1 actggcgtat cgcaaaaatc tgctctatgt ccactcactg cgcgagttga cggtcagcgc
       61 tgttctaccc ggcattcgct ctttgctggc agatctccgt gcacagcaga tcccggttgg
      121 gctggcttct gtctccctga atgcgccgac gattttagcg gcgctggagc tgcgcgagtt
      181 tttcaccttc tgcgcggatg cttcccaact taaaaactcg aaaccggacc cggaaatctt
      241 tctcgccgcc tgtgccgggc tgggcgtgcc gccgcaggca tgtatcggca ttgaagatgc
      301 gcaggcgggc attgacgcca ttaacgccag cggtatgcgc tcggtgggga tcggcgcggg
      361 cttaaccggg gcgcaattac tgttgccttc aacggaatca ctcacctggc cgcggttatc
      421 ggccttctgg caaaacgtat agcaaaggaa tcaacatggc tcagctttcg ttacaacata
      481 ttcaaaaaat ctacgataac caggtgcatg tggtgaagga cttcaacctg gaaattgccg
      541 ataaagagtt catcgtgttt gtcggcccgt cgggctgcgg taagtcgacc accctgcgca
      601 tgattgccgg gcttgaggag atcagcggcg gcgatctgtt gatcgacggc aaacgaatga
      661 atgacgttcc agccaaagca cgcaatatag cgatggtgtt ccagaactac gcgttgtatc
      721 cgcatatgac ggtttacgac aacatggcgt ttggcctgaa gatgcaaaaa atcgccaaag
      781 aggtgattga tgagcgggtg aactgggcgg cgcaaattct cggcctgcgt gagtacctga
      841 aacgcaagcc gggcgcgctt tccggcgggc aacgccagcg tgtggcgctc gggcgggcga
      901 ttgtgcgcga agcgggcgtg tttttaatgg atgaaccgct ctctaacctt gatgccaagc
      961 tgcgcgtgca aatgcgcgca gagatcagca agctgcacca gaagctgaat accaccatga
     1021 tctacgtgac ccacgatcag accgaagcga tgaccatggc gacgcggatt gtgattatga
     1081 aggatgggat tgttcagcaa gttggtgcgc cgaaaacggt ttataaccaa ccagcgaata
     1141 tgtttgttgc cggatttatt ggatcaccgg cgatgaattt tattcgtggc acgatcgatg
     1201 gcgataaatt cgttacggaa acgcttaaat taaccattcc cgaagcgaaa ttagcggttc
     1261 tgaaaacaca gggaagtttg cataagccca tcgtgatggg aatacgtccg gaagatattc
     1321 atccggacgc gcaagaggaa aataacattt ccgccaaaat tagcgtggca gaattaaccg
     1381 gtgcggaatt tatgctctac accacggttg gggggcacga gttagtggtc cgtgctggtg
     1441 cgttaaatga ttatcatgca ggagaaaata tcactattca ttttgatatg acgaaatgtc
     1501 atttctttga tgcagaaacg ggaatagcac ttcgctaaat acagggggaa ggcattcccc
     1561 caggataata caaggaacaa taatgaaaaa gttattaccc tgtaccgcac tggtgatgtg
     1621 tgcgggaatg gcctgcgcac aggccgagga aaagaacgac tggcacttta atatcggcgc
     1681 gatgtacgaa atagaaaacg tcgagggtta tggcgaagat atggatgggc tggcggagcc
     1741 ttcagtctat tttaatgccg ccaacgggcc gtggagaatt gctctggcct attatcagga
     1801 agggccggta gattatagcg cgggtaaacg tggaacgtgg tttgatcgcc cggagctgga
     1861 ggtgcattat cagttcctcg aaaacgatga tttcagtttc ggcctgaccg gtggtttccg
     1921 taattatggt tatcactacg ttgatgaacc gggtaaagac acggcgaata tgcagcgctg
     1981 gaaaatcgcg ccagactggg atgtgaaact gacggacgat ttacgtttca acggttggtt
     2041 atcgatgtat aaatttgcca acgatctgaa cacgaccggt tacgctgata cccgagtcga
     2101 aacggaaaca ggcctgcaat ataccttcaa cgaaacggtt gccttgcgag tgaactatta
     2161 tctcgagcgc ggctttaata tggacgacag ccgcaataac ggtgagtttt ctacgcaaga
     2221 aattcgcgcc tatttgccgc tgacgctcgg caaccactcg gtgacgccgt atacgcgcat
     2281 tgggctggat cgctggagta actgggactg gcaggatgat attgaacgtg aaggccatga
     2341 ttttaaccgt gtaggtttat tttacggtta tgatttccag aac
//
LOCUS       DAMZET010000322         2373 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.322, whole genome shotgun sequence.
ACCESSION   DAMZET010000322 DAMZET010000000
VERSION     DAMZET010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.322"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1583
                     /gene="tynA"
                     /locus_tag="POB34_004822"
     CDS             <1..1583
                     /gene="tynA"
                     /locus_tag="POB34_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="catalyzes the formation of phenylacetaldehyde from
                     2-phenylethylamine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HDI5921640.1"
                     /translation="SEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKV
                     ISYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDSRDRVAPAV
                     KPMQIIEAEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGS
                     LGGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGV
                     PMEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHEN
                     GTIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLD
                     VDGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNK
                     ENRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPE
                     GKYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLK
                     PWNFFDETPTLGALKKDK"
     gene            complement(1641..>2373)
                     /gene="feaB"
                     /locus_tag="POB34_004823"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(1641..>2373)
                     /gene="feaB"
                     /locus_tag="POB34_004823"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HDI5921641.1"
                     /translation="GIARTAADHLTRVTLELGGKNPAIVLKDADPQWVIEGLMTGSFL
                     NQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQVGPGMSPVAQINPLVSRAHCDKVC
                     SFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVNPDAKLRLTREEVFGPVVNLVRVAD
                     GEEALQLANDTEYGLTASVWTQNLSQALEYSDRLQAGTVWVNSHTLIDANLPFGGMKQ
                     SGTGRDFGPDWLDGWCETKSVCVRY"
BASE COUNT          621 a          624 c          579 g          549 t
ORIGIN      
        1 acagtgaaga atttgccgct gccgtgaaga aacgcggtat tactgatgcg aaaaaagtga
       61 ttaccacgcc gctgaccgta ggttatttcg atggtaaaga tggcctgaaa caagatgccc
      121 ggttgctcaa agtcatcagc tatcttgatg tcggtgatgg caactactgg gcacatccca
      181 tcgaaaacct ggtggcggtc gttgatttag aacagaaaaa aatcgttaag attgaagaag
      241 gtccggtagt tccggtgcca atgaccgcac gcccatttga tagccgtgac cgcgttgctc
      301 cggcagttaa gcctatgcaa atcattgagg ctgaaggtaa aaattacacc attactggcg
      361 atatgattca ctggcggaac tgggattttc acctcagcat gaactctcgc gtcgggccga
      421 tgatctccac cgtgacttat aacgacaatg gcaccaaacg caaagtcatg tacgaaggtt
      481 ctctcggcgg catgattgtg ccttacggtg atcctgatat tggctggtac tttaaagcgt
      541 atctggactc tggtgactac ggtatgggca cgctaacctc accaattgct cgtggtaaag
      601 atgccccgtc taacgcagtg ctccttaatg aaaccatcgc cgactacact ggcgtgccga
      661 tggagatccc tcgcgctatc gcggtatttg aacgttatgc cgggccggag tataagcatc
      721 aggaaatggg ccagcccaac gtcagtaccg aacgccggga gttagtggtg cgctggatca
      781 gtacagtggg taactatgac tacatttttg actggatctt ccatgaaaac ggcactattg
      841 gcattgatgc cggtgctacg ggcatcgaag cggtgaaagg tgttaaagcg aaaaccatgc
      901 acgatgagac ggcgaaagat gacacgcgct acggcacgct tatcgatcac aatatcgtgg
      961 gtactacaca ccaacatatt tataatttcc gcctcgatct ggatgtagat ggcgagaata
     1021 acagcctggt ggcgatggac ccagtggtaa aaccgaatac tgccggtggc ccacgcacca
     1081 gtaccatgca agttaatcag tacaacatcg gcaatgaaca ggatgccgcg cagaagtttg
     1141 atccgggcac gattcgtctg ttgagtaacc cgaacaaaga gaaccgcatg ggcaatccag
     1201 tttcctatca aattattcct tatgcaggtg gtactcaccc ggtagcaaaa ggtgcccagt
     1261 tcgcgccgga cgagtggatc tatcatcgtt taagctttat ggacaagcag ctttgggtaa
     1321 cgcgttatca tcctggcgag cgtttcccgg aaggcaaata tccgaaccgt tctactcatg
     1381 acaccggtct tggacaatac agtaaagata acgagtcgct ggacaacacc gacgccgttg
     1441 tctggatgac caccggcacc acacatgtgg cccgcgccga agagtggccg attatgccga
     1501 ccgaatgggt acatactctg cttaaaccat ggaacttctt tgacgaaacg ccaacgctag
     1561 gggcgctgaa gaaagataag tgattgtttc agacaaaaaa acggcaccag gtgccgtttt
     1621 ttacttatga gcgaaccaga ttaataccgt acacacaccg acttagtttc acaccaaccg
     1681 tccagccagt cggggccaaa atcacggccc gttcctgact gcttcatccc accaaacggt
     1741 aagttagcgt caattaaggt atggctgttt acccacaccg tccctgcctg taagcgatcg
     1801 ctatattcca gagcctggga gagattttgc gtccagacac tggcagttaa gccatattcc
     1861 gtgtcgtttg ccagttgtaa cgcctcttct ccatccgcta ctcgcaccag gtttaccacc
     1921 ggaccaaaca cctcttcacg agttaagcgc aatttagcat cgggatttac caccagcgtt
     1981 ggcgcaacat aatacccctc tccggctggt ccattcgacc cgcgaatcag ctctgcttgc
     2041 tgtgcctgcg catcgtcgag gaatgaacac actttgtcgc agtgcgcacg agaaaccaaa
     2101 gggttaatct gtgcaacagg tgacatcccc ggtcccactt gcaacgattt taccgcctgc
     2161 tcaaatccac taaccagcgt gtcaaacaac ggcgcttcaa tataaattcg cgaactggcg
     2221 gcgcatactt gcccttgatt caggaagctt ccggtcatca agccttcaat aacccattgc
     2281 ggatcagcat cttttaatac aattgccggg tttttaccgc ccagttccag cgttacacgc
     2341 gttaagtgat cagcagcagt tctggcaata cct
//
LOCUS       DAMZET010000323         2341 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.323, whole genome shotgun sequence.
ACCESSION   DAMZET010000323 DAMZET010000000
VERSION     DAMZET010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.323"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            451..1146
                     /gene="minC"
                     /locus_tag="POB34_004824"
     CDS             451..1146
                     /gene="minC"
                     /locus_tag="POB34_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDI5921642.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1170..1982
                     /gene="minD"
                     /locus_tag="POB34_004825"
     CDS             1170..1982
                     /gene="minD"
                     /locus_tag="POB34_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDI5921643.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            1986..2252
                     /gene="minE"
                     /locus_tag="POB34_004826"
     CDS             1986..2252
                     /gene="minE"
                     /locus_tag="POB34_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDI5921644.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
BASE COUNT          653 a          520 c          592 g          576 t
ORIGIN      
        1 tgacctggac gagttacgat gacctgacag aagtatcaga cgtcttctgc ggtttctatg
       61 atagagcaat tctgtggcgg atattaagat aacgggtatc tgttctttaa tgatgccgca
      121 aaaaatggaa agttaagaaa gcctggcgtg gtgaaaaaat acacttgcct caatactgga
      181 tatttaaaca ggtctggaga tgctgtcacg attagggtgc ggaaaaagga agcgtaaata
      241 agaacaatca aaagtacgac ggcaatgggt tgattgacaa gggtattttt taagctatga
      301 atcagcgcca tttatcacag aatagacttt tactctgaat aaatgggagg gtgacttgcc
      361 tcaatataat ccagactata acatgcctta tagtcttcgg aacatcatcg cgcgctggcg
      421 atgattaata gctaattgag taaggccagg atgtcaaaca cgccaatcga gcttaaaggc
      481 agtagcttca ctttatctgt ggttcatctg catgaggcag aacctaaggt tatccatcag
      541 gcgctggaag acaaaatcgc tcaggccccc gcatttttaa aacatgcccc cgttgtactc
      601 aacgtcagtg cactggaaga cccggtaaac tggtcagcga tgcataaggc ggtttcggca
      661 accggtttgc gggttattgg cgtaagtggc tgcaaagatg cgcaacttaa agccgaaatt
      721 gaaaagatgg ggctgcctat cctgacggaa ggaaaggaaa aagcgccgcg tccagctccc
      781 gcaccacagg ctccagcgca aaatacaacg ccggtcacaa aaacgcgttt aatagatacc
      841 ccggtgcgtt ccggtcagcg tatttatgct ccacaatgtg atctgattgt tacaagccac
      901 gttagcgctg gggccgaatt gattgccgat gggaacattc atgtctatgg catgatgcgc
      961 ggtcgtgcgc ttgcaggggc aagtggtgac cgggaaacgc aaatattttg tacgaacctg
     1021 atggcggaac tggtgtccat cgcaggtgaa tactggctga gtgatcaaat cccagcagaa
     1081 ttttatggca aagcggcgcg actgcagtta gtcgaaaacg ctttgaccgt tcaaccgtta
     1141 aattgatccc tttttaacaa ggaatttcta tggcacgcat tattgttgtt acttcgggca
     1201 aagggggtgt tggtaagaca acctccagcg cggccatcgc cactggtttg gcccagaagg
     1261 gaaagaaaac tgtcgtgata gattttgata tcggcctgcg taatctcgac ctgattatgg
     1321 gttgtgaacg ccgggtcgtt tacgatttcg tcaacgtcat tcagggcgat gcaacgctaa
     1381 atcaggcgtt aattaaagat aagcgtactg aaaatctcta tattcttccg gcatcgcaaa
     1441 cacgcgataa agatgccctc acccgtgaag gggtcgccaa agttcttgat gatctgaaag
     1501 cgatggattt tgaatttatc gtttgtgact ccccggcagg gattgaaacc ggtgcgttaa
     1561 tggcactcta ttttgcagac gaagccatta ttaccaccaa cccggaagtc tcctcagtac
     1621 gcgactctga ccgtatttta ggcattctgg cgtcgaaatc acgccgcgca gaaaatggcg
     1681 aagagcctat taaagagcac ctgctgttaa cgcgctataa cccaggccgc gtaagcagag
     1741 gtgacatgct gagcatggaa gatgtgctgg agatcctgcg catcaaactc gtcggcgtga
     1801 tcccagagga tcaatcagta ttgcgcgcct ctaaccaggg tgaaccggtc attctcgaca
     1861 ttaacgccga tgcgggtaaa gcctacgcag ataccgtaga acgtctgttg ggagaagaac
     1921 gtcctttccg cttcattgaa gaagagaaga aaggcttcct caaacgcttg ttcggaggat
     1981 aagttatggc attactcgat ttctttctct cgcggaagaa aaacacagcc aacattgcaa
     2041 aagaacggct gcagattatt gttgctgaac gccgtcgcag cgatgcagaa ccgcattatc
     2101 tgccgcagtt gcgtaaagat attcttgagg tcatttgtaa atacgtacaa attgatcctg
     2161 agatggtaac cgtacagctt gagcaaaaag atggcgatat ttctattctt gagctgaacg
     2221 tgaccttacc ggaagcagaa gagctgaaat aagcccgctg taaaaacgca tttatcttca
     2281 aggcagagtc atttctgcct tgagtttttc atccctctca tccacgttgt ggtaaagcgg
     2341 c
//
LOCUS       DAMZET010000324         2332 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.324, whole genome shotgun sequence.
ACCESSION   DAMZET010000324 DAMZET010000000
VERSION     DAMZET010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.324"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(131..976)
                     /gene="nhoA"
                     /locus_tag="POB34_004827"
     CDS             complement(131..976)
                     /gene="nhoA"
                     /locus_tag="POB34_004827"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HDI5921645.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGDYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            1149..1718
                     /locus_tag="POB34_004828"
     CDS             1149..1718
                     /locus_tag="POB34_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDI5921646.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(1722..1949)
                     /gene="pptA"
                     /locus_tag="POB34_004829"
     CDS             complement(1722..1949)
                     /gene="pptA"
                     /locus_tag="POB34_004829"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HDI5921647.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(2000..2332)
                     /locus_tag="POB34_004830"
                     /pseudo
     CDS             complement(2000..2332)
                     /locus_tag="POB34_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          590 a          582 c          534 g          626 t
ORIGIN      
        1 ttttgtttca tgccgaatgg gcaattaaac tctgaaaccg ttgcctgatg cgcttcgctt
       61 atcaggtcta caaccgagct aatgtatgcc ggataaggcg tttacgccgc atccggcaac
      121 ctgacataaa ttattttccc gcctccgggt gcgtatcaaa agccgccatc accagcgcta
      181 actcatccac ggtaaagcca tgtttcgcat catccacgcc cagaccaaac tgttcttgca
      241 tcacagcata taatgacgcc acatccggta gatttcgctg ctccaccgcg tgcccatttt
      301 cataatgggt aaaatgaaaa ttggtcagtg tcagcttgcc gccgtccggc aaatggcggc
      361 acatcagcaa atgatggcga aaatgcgact gcggccagtg cgccgaccag aaattgccca
      421 tcacataatc gctttgttgc tgctcgcaga gatcaaaacg gtacatcgac tgccagtgct
      481 gatgatgatt aaactgcaac acccaatcat caccctcctg caacaaccga taatctccgt
      541 gtggcgtggt ctgcacgaga tcggaaacta aacgaatcgg cgcggttagc gtctgcccac
      601 cgaaaccgac atcagcaatc catttttcct cttccagttc caccaacagc aaacgatggg
      661 tgcgcggcgg taatgctggc ggatttgata acactacgcg ccctaacaag ctgcgaacgt
      721 taaactccag ctcgcgtaac acccgctcaa acacgccatt ctgctcaaaa cagtaaccgc
      781 cacgacgggc tatcaccagt ttctcttcca gcgattgatc atcaagctgt atttccctcg
      841 gcagcaaaac gtcgaggttt tcaaacggaa tagtgcaatt gtgtttcagg tgcaatgcac
      901 gaagcgtatc aatattgacc gcagcagctc ccgaccagtt aatacgggca aaatagtgat
      961 tcagaatggg cgtcatcagg cgtttccctt agtggttttc tcaacttata acctggaata
     1021 gacgccgcac cggctatttt gtgcgatcgc ggacatgcat cacgtttgca aatcgccaat
     1081 cccgttaatt tcgttactta ttgcgttacg gactacgctt aaaaaaacct tcttcgcgag
     1141 gcgataccat gagccgattc atccccatag aattacacca tgccagccgt ctgttaaatc
     1201 atggcccaac tgtcatgatc accagtttcg atgagcaatc ccagcggcgt aacattatgg
     1261 cagcagcctg gtcaatgccg gtggagtttg aaccgccacg cgtggcgatt gtggtagata
     1321 aatcgacatg gaccagggag ttgattgaac gtaacggtaa atttggcatc gttatcccgg
     1381 gcgttgcagc aactaactgg acgtgggcag tgggaagcgt gtcgggtcgt gaagaagata
     1441 aatttaattg ctatggcatt ccggtggtga aaggtccggt gcttggtttg ccacttgttg
     1501 aagagaaatg cctggcgtgg atggagtgcc gcctgctacc tgcgacttct gcgcaacaaa
     1561 aatacgacac gctgtttggc gaggtggtat cagcagcggc agacgcacgg gtatttgtcg
     1621 aaggccgctg gcagtttgat gatgataagc tcaatacgtt gcatcattta ggtgctggca
     1681 cgtttgttac cagcggcaag cgagttacgg cgggttaagc attacatgct gtaaccaggt
     1741 ttctttatca aagcctccat ttggggcgcg atttcggcat cccagatagc ttgccaagat
     1801 tctggttgaa tctgctgtag agcaatgctt atcgaactgt ctttactgtt cagatggcga
     1861 ataataacgt cggtaatatc tgcagcaagt gctgcttttt gttgttcgtc cagttcacgc
     1921 ggaaaacatt taatgtcgat gtgcggcatg attgtgctcc ttttgaaaga tgtcagtaaa
     1981 attaacacac attgtgaatt ttcagtcctt tccattcaaa cgtgaaatca ataagttcat
     2041 cagggacatc gcaaacaata tgaatacgga tttcttctct gccgtgactc ttttcacaaa
     2101 ttgcgtaact gtcatgctct ggattattta attctttcag cggaaatttt tcctcaaagg
     2161 ctttatttag ccacccctgg tttcctttca ccgcgaataa ataatcacct ccctgttttt
     2221 gtatcttctc tgctatatct ttctggcaac ccatcgcatc agttgtgatg atttttcgtt
     2281 taatatccag catgttaaga agttctggga tagctgtaat ctcattagat tt
//
LOCUS       DAMZET010000325         2296 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.325, whole genome shotgun sequence.
ACCESSION   DAMZET010000325 DAMZET010000000
VERSION     DAMZET010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.325"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>2296
                     /locus_tag="POB34_004831"
     CDS             <1..>2296
                     /locus_tag="POB34_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001475310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDI5921648.1"
                     /translation="THAMLQSDSENTTSVKVGEQSIGGLAMNGGTLIFDTDIPAATLA
                     EGYISVDTLVVGAGDYTWKGRNYQVNGTGDVLIDVPKPWNDPMANNPLTTLNLLEHDD
                     SHVGVQLVKAQTVIGSGGSLTLRDLQGDEVEADKTLHIAQNGTVVAEGDYGFRLTTAP
                     GDGLYVNYGLKALNIHGGQKLTLAEHGGAYGATADMSAKIGGEGDLAINTVRQVSLSN
                     GQNDYQGATYVQMGTLRTDADGALGNTRELNISNAAIVDLNGSTQTVETFTGQMGSTV
                     LFKEGALTVNKGGISQGELTGGGNLNVTGGTLAIEGLNARYNALTSISPNAEVSLDNT
                     QGLGRGNIANDGLLTLKNVTGELRNSISGKGIVSATARTDVELDGDNSRFVGQFNIDT
                     GSALSVNEQKNLGDASVINNGLLTISTERSWAMTHSISGSGDVTKLGTGILTLNNDSA
                     AYQGTTDIVGGEIAFGSDSAINMASQHINIHNSGVMSGNVTTAGDVNVMPGGTLRVAK
                     TTIGGNLENGGTVQMNSEGGKPGNVLTVNGNYTGNNGLMTFNATLGGDNSPTDKMNVK
                     GDTQGNTRVRVDNIGGVGAQTVNGIELIEVGGNSAGNFALTTGTVEAGAYVYTLAKGK
                     GNDEKNWYLTSKWDGVTPPDTPDPINNPPVVDPEGPSVYRPEAGSYISNIAAANSLFS
                     HRLHDRLGEPQYTDSLHSQGSASSMWMRHVGGHERSRAGDGQLNTQANRYVLQLGGDL
                     AQWSSNAQDRWHLGVMAGYANQHSN"
BASE COUNT          600 a          531 c          694 g          471 t
ORIGIN      
        1 tactcacgcg atgttgcagt ctgacagtga aaataccaca tcggtaaaag tgggagagca
       61 atccattggt ggactggcca tgaatggcgg tacgctcatt ttcgatacgg atattcctgc
      121 tgcgacgctt gcagagggat atatcagcgt cgatacgctg gttgtcggcg cgggtgacta
      181 cacctggaaa ggccgtaact atcaggtaaa cgggacgggc gacgtgctta tcgacgtgcc
      241 taaaccgtgg aatgatccca tggcgaataa ccctctgacg acgctcaatt tgctggaaca
      301 cgacgatagc catgtcggcg ttcaactggt gaaggcgcaa acggttattg ggtcgggtgg
      361 ctcattaacg ttacgtgatt tacagggcga cgaggtggaa gcggacaaaa cgttacacat
      421 tgcgcaaaac ggaacggtgg tcgccgaggg tgattatgga ttccgcctca cgaccgcacc
      481 aggtgatggt ttgtacgtta actatgggct gaaagcgctg aacatccatg gtgggcaaaa
      541 gctgacgtta gccgaacatg gcggagccta tggcgcaacg gccgatatgt cggcaaaaat
      601 cggtggtgaa ggggatctgg caatcaatac ggtgcgacag gtttcgcttt ccaacggtca
      661 gaacgactat cagggggcaa cctacgttca gatggggaca ttacgtaccg atgcggatgg
      721 tgcacttggc aacacccggg aactgaacat cagcaacgcg gccatcgtcg atcttaatgg
      781 atcgacgcag acggtagaga cattcaccgg gcagatgggt tcgactgttt tgttcaaaga
      841 gggggcgctg acggtaaata aaggtgggat cagtcagggt gaactgacag gtggcggaaa
      901 cctgaatgtt acagggggaa cgctggctat cgaggggctt aatgcacgct acaatgcgtt
      961 aaccagcatt agcccaaatg cggaagtcag cctcgataat actcaggggt taggcagagg
     1021 aaatattgcc aatgacggtc tgttaacgct aaaaaacgtg actggcgaac tgcgtaatag
     1081 cataagcggg aagggtatcg taagcgcaac cgccaggaca gatgtagagc tggatggcga
     1141 taatagccgc tttgtggggc aattcaacat tgatacaggc agcgcgctca gcgtcaacga
     1201 gcagaaaaac ctgggtgatg cttccgttat caataatggc ctgctcacca tctccactga
     1261 gcgtagctgg gcgatgacgc acagtatcag cggtagcggt gatgtgacaa aactgggtac
     1321 cgggatcctg actcttaaca acgattccgc ggcgtatcag ggtacgacgg atatcgtggg
     1381 gggggaaatt gctttcggtt ccgactctgc cattaatatg gcaagtcaac acattaatat
     1441 ccataacagc ggtgtgatgt cgggaaatgt caccactgca ggtgatgtga acgttatgcc
     1501 tggggggaca ctgcgtgtcg ctaaaaccac aatcggcggc aacctggaga atggcggcac
     1561 ggttcaaatg aatagcgaag gggggaaacc ggggaatgta ctgaccgtta acggcaacta
     1621 taccggaaac aatggcctga tgacgttcaa cgcgacgctg ggcggcgata attcgcccac
     1681 cgataagatg aacgtgaaag gcgataccca agggaacact cgcgttcggg ttgataacat
     1741 tggcggcgtc ggtgcacaaa cggtcaacgg tattgaactc attgaggttg gcggtaattc
     1801 tgcaggtaac ttcgcgctga caaccggaac tgtcgaagct ggggcttacg tctacacgct
     1861 ggctaaaggg aaggggaatg acgagaaaaa ctggtatctg accagtaaat gggacggcgt
     1921 aacgccaccg gatacacccg accccatcaa taatccccct gttgtggatc cggaaggccc
     1981 atcagtttat cgcccggagg ccggaagcta tatcagcaac attgccgcag ccaactcgct
     2041 gtttagccat cgtttacacg accgtttggg tgagccgcag tatacagatt cactgcattc
     2101 tcaggggtcg gcaagcagta tgtggatgcg tcatgtcgga gggcacgaac gttcaagggc
     2161 cggtgacggt cagctaaata ctcaggctaa ccgctatgta ttgcagctag gcggcgattt
     2221 ggcgcagtgg agtagcaacg cgcaggatcg ctggcatctt ggcgtgatgg caggctacgc
     2281 caatcagcac agtaat
//
LOCUS       DAMZET010000326         2268 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.326, whole genome shotgun sequence.
ACCESSION   DAMZET010000326 DAMZET010000000
VERSION     DAMZET010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.326"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..193)
                     /locus_tag="POB34_004832"
     CDS             complement(<1..193)
                     /locus_tag="POB34_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDI5921649.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFF"
     gene            455..1654
                     /gene="lolC"
                     /locus_tag="POB34_004833"
     CDS             455..1654
                     /gene="lolC"
                     /locus_tag="POB34_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HDI5921650.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            1647..>2268
                     /gene="lolD"
                     /locus_tag="POB34_004834"
     CDS             1647..>2268
                     /gene="lolD"
                     /locus_tag="POB34_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HDI5921651.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQ"
BASE COUNT          528 a          564 c          618 g          558 t
ORIGIN      
        1 taaagaaaaa aaccggcata cgaaaggggg caaggtaaag attgaaatag atccagcatt
       61 tgctcaggac ttccgataac ggatgctgaa aagtggtcag atgcggataa aaggtaatga
      121 ccgagtgata aatcaccacc agacaaatac ataacccttt gatctggtta atccatagct
      181 ctttttgttt catcaggaca acacgctctt ttttaattta catgcggttg atcctgcggg
      241 ttatgacccg aggtgtaatg ggtatctgtc tgtattggat gatttttcag attaagatca
      301 ggcggcaaga ttgatgataa aacatggcaa tttagccgat tgatttacgg gggcttttca
      361 gattagccct gacgatcact tacagttcag acgtttaccc atcttgcttt cgcttatata
      421 ctcgtgtctt tgctacagca accagacgga tttcatgtac caacctgtcg ctctatttat
      481 tggcctgcgt tacatgcgtg ggcgtgcagc ggatcgcttc ggtcgtttcg tctcctggct
      541 ttctaccatc ggcattaccc tcggggtgat ggcgctggtc acagtattgt cagtgatgaa
      601 cggctttgag cgcgagctgc aaaacaacat ccttggcctg atgccacagg caattctctc
      661 ttctgagcat ggctctctta acccgcagca actcccggaa acggcagtca aactggacgg
      721 cgttaatcgc gtcgcaccta ttactaccgg tgatgtggta ctgcaaagcg cgcgcagcgt
      781 ggcggtcggg gtgatgctcg gtatcgaccc ggcgcaaaaa gatccactta caccgtatct
      841 ggtcaatgtg aaacaaactg acctcgagcc ggggaaatat aatgtcatcc tcggcgaaca
      901 acttgcctca cagctaggcg ttaatcgcgg tgatcaaatc cgcgtgatgg taccatctgc
      961 cagccagttc acgccgatgg ggcgtattcc aagccagcgc ctgttcaatg tgattggcac
     1021 tttcgccgcc aacagtgaag tcgatggcta tgaaatgctg gtgaatattg aggatgcctc
     1081 gcgtctgatg cgttatccgg caggcaatat taccggctgg cgtttgtggc tggatgagcc
     1141 gctgaaagtt gactctttaa gtcagcaaaa actgcctgaa ggcagcaaat ggcaggactg
     1201 gcgtgaccgt aaaggcgagc tgttccaggc cgtacgcatg gaaaaaaata tgatgggctt
     1261 gctgctgagc ctgattgtcg ccgttgcggc gtttaacatt attacctcac tagggctgat
     1321 ggtaatggag aagcagggcg aagtcgcgat cctgcaaacg caaggcttaa ctccgcgaca
     1381 aatcatgatg gtctttatgg tgcaaggggc cagcgccggg attatcggtg cgatcctcgg
     1441 tgcggcgctt ggcgcactgc ttgccagcca gttaaataat ctgatgccga taatcggcgt
     1501 cctgcttgat ggcgcggcgc tgccggtggc tatcgaacct ttacaggtca ttgttattgc
     1561 gctggtggcg atggctatcg cgctgctgtc tacgctttac ccttcatggc gcgctgccgc
     1621 cacacaaccc gctgaggctt tacgttatga ataagatcct gttgcaatgc gacaacctgt
     1681 gcaaacgcta tcaggaaggc agtgtgcaaa ccgatgtact gcacaatgtc agtttcagcg
     1741 tgggcgaagg tgaaatgatg gcgatcgtcg gtagctctgg ttccggtaaa agtaccttgc
     1801 tgcacctgct gggcgggctg gatacaccaa cctccggcga tgtgatcttt aacggtcaac
     1861 cgatgagcaa actgtcttcg gcggcgaaag cagaactgcg caaccagaag ctgggcttta
     1921 tttatcagtt tcaccacctg ctgccggatt ttactgccct ggaaaacgtg gctatgccac
     1981 tgctgattgg caagaaaaag cccgctgaaa tcaacagccg tgcgctggag atgttaaaag
     2041 cggtggggct ggagcatcgt gcgaatcacc gcccatctga actttctggc ggcgaacgcc
     2101 agcgtgtggc gattgcccgt gcgctggtca ataatccgcg tctggtactt gcggatgagc
     2161 ctaccggtaa cctcgatgcg cgtaacgccg acagcatctt ccagttgctt ggggaattga
     2221 atcgcttgca gggcaccgcc ttcctggtgg ttactcacga cctgcaac
//
LOCUS       DAMZET010000327         2238 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.327, whole genome shotgun sequence.
ACCESSION   DAMZET010000327 DAMZET010000000
VERSION     DAMZET010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.327"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            37..>2238
                     /locus_tag="POB34_004835"
     CDS             37..>2238
                     /locus_tag="POB34_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HDI5921652.1"
                     /translation="MVVNTVASENPDEAGRYSMDVEYGQYSVILLVEGFPPSHAGTIT
                     VYEDSRPGTLNDFLGAMTEDDARPEALRRFELMVEEVARNASAVAQNTAAAKKSASDA
                     STSAREAATHATDAADSARAASTSAGQAASSAQSASSSAGTASTKATEASKSAAAAES
                     SKSAAATSAGAAKTSETNAAASQQSAATSASTATTKASEAATSARDASASKEAAKSSE
                     TNASSSASSAASSATAAGNSAKAAKTSETNADNSAQAAADSQTASANSATAAKKSETN
                     AKNSEAAAKSSETNAKASETNAKSSETNAAKSAADALNYRNQAQVIVGDNIGLGEVAM
                     TCTDISVEPSGYIGFVRIYISAKGYPSVASSAGDNLISGYIVRSEVSTKRYSGLFVGS
                     DTKSLYSYIYSQSSGPQWIRHTRRDELSRFGQNESTTRMYGPTGDRFFEIHTNGSWGV
                     YDSSSGQWSPLGLAQGGTGGRTIEQARANLRVMYEQKAGLANTDLNTLTGEYSGFYQQ
                     PTSAYATEELNYPIGLAGALIVLQTRANTASSCVQVYYPYNNPGITYRRIYEGGSGTW
                     SEWKRDVSTERVEEGKETTYVYSTYSSGAPRLQVSKSGLWGCHNGTGWLPLAVGQGGT
                     GATTVEDARNNLSLGESSAVKFKNLTLTEALDTTLGLLTKTGRDWNTQHTDNINKFIP
                     IAGSTNGPAGSMVLGGIHVQFSKNYAVQFGGRNSGFWGRTIENGTTQEWKKL"
BASE COUNT          656 a          527 c          577 g          478 t
ORIGIN      
        1 acaatccagc tgaaagcaaa acgtaacagc accacggtgg tggtgaacac ggtggcctca
       61 gaaaatccgg atgaagccgg gcgttacagc atggacgttg agtacggtca gtacagcgtt
      121 attctgttgg tggaaggatt cccgccgtca catgccggga ccatcaccgt gtatgaagat
      181 tcccgacccg gtacgctgaa tgattttctc ggtgccatga cggaggatga tgcccgtccg
      241 gaggctctgc gccgttttga actgatggtg gaagaggtgg cgcgtaacgc gtccgcggtg
      301 gcacagaaca cggcagccgc gaagaaatca gccagcgatg ccagcacatc agcccgtgag
      361 gcggcaaccc atgcgactga tgctgcagat tcagcacgtg cagccagcac gtcagccgga
      421 caggccgctt cgtcggctca gtcagcgtct tccagcgcag gaacggcatc aacaaaggcc
      481 actgaagcct caaaaagtgc tgccgctgca gagtcctcaa aaagcgcggc ggctaccagt
      541 gccggtgcgg cgaaaacgtc agaaacgaat gcggcagcgt cacaacaatc agcagccact
      601 tctgcatcca ccgcgaccac gaaagcgtca gaagctgcca cctcagcccg ggatgcgtcg
      661 gcctcaaaag aggcagcgaa atcatcagaa acgaacgcat catcaagtgc cagtagtgca
      721 gcttcctcgg caacggcggc aggaaattcc gcgaaggccg caaaaacgtc tgagacgaat
      781 gcagataaca gcgcacaggc ggcagcagac tcacaaactg catcagcaaa ctccgcgaca
      841 gcagccaaaa aatcagaaac caacgcgaaa aatagcgagg cagcagcaaa gagcagcgaa
      901 accaacgcta aagccagcga aactaatgct aaatccagtg aaacaaacgc ggcgaaatct
      961 gcggcggacg cacttaatta tcgcaaccag gcgcaagtaa ttgttggcga taatatcggt
     1021 cttggtgaag ttgccatgac ctgcactgat atctctgttg agccatcagg ttatattgga
     1081 tttgtcagga tttacatctc agcgaaaggt tatccatcag tagcgtctag tgccggtgat
     1141 aatcttattt ctggttatat agttagatca gaagtgtcaa caaaaagata ttccggtcta
     1201 tttgttggtt cagatacaaa atcattgtat tcgtacattt acagccaaag ttctggcccg
     1261 caatggatac gtcatacccg cagagatgaa ttaagccgct ttggtcaaaa tgaatcaaca
     1321 actcgtatgt atggtccaac tggtgatcgt ttctttgaaa tccatactaa cgggagttgg
     1381 ggagtttatg attcttcttc tggacaatgg tctccgttag gtttagccca gggcggtact
     1441 ggtggaagaa ctatcgagca agcacgtgcg aacttgcggg taatgtatga gcaaaaagct
     1501 ggccttgcta atactgacct aaacaccctt accggtgaat attctggttt ctatcaacaa
     1561 ccaacgagcg cttacgcaac agaagaatta aattacccaa tcggtctggc gggcgcttta
     1621 atagtgctcc aaacgagagc caacactgct tcttcctgcg ttcaggtgta ctacccttat
     1681 aataatccgg gaattactta tagacgaata tatgaaggag gaagcggtac ctggtctgaa
     1741 tggaagagag atgtatcaac agaaagggtt gaagagggaa aagaaacaac ttacgtatat
     1801 tctacgtatt cttcaggcgc accacgctta caggtttcca aatctggttt gtggggttgt
     1861 cataatggca ctggctggtt gccattagct gttgggcaag gaggtacagg tgcgacaaca
     1921 gtagaagatg cgcgaaacaa cttaagtctt ggcgaaagta gcgcagttaa atttaaaaac
     1981 cttactttaa ccgaagcgct cgacacgaca ttaggactgc ttacaaaaac aggacgagac
     2041 tggaacacgc agcatactga taacattaat aaatttatac caattgcagg cagtacaaac
     2101 ggcccggcag gttctatggt tcttggcggc attcatgttc aatttagtaa aaattatgct
     2161 gtgcagttcg gaggccgcaa ttccggtttt tggggaagaa caattgaaaa tggaacgacg
     2221 caggaatgga agaaatta
//
LOCUS       DAMZET010000328         2216 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.328, whole genome shotgun sequence.
ACCESSION   DAMZET010000328 DAMZET010000000
VERSION     DAMZET010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.328"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..366
                     /gene="ogt"
                     /locus_tag="POB34_004836"
     CDS             <1..366
                     /gene="ogt"
                     /locus_tag="POB34_004836"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDI5921653.1"
                     /translation="YERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVW
                     KTLRTIPCGQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGY
                     AGGVQRKEWLLRHEGYLLL"
     gene            561..1313
                     /gene="fnr"
                     /locus_tag="POB34_004837"
     CDS             561..1313
                     /gene="fnr"
                     /locus_tag="POB34_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HDI5921654.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            1465..>2216
                     /gene="uspE"
                     /locus_tag="POB34_004838"
     CDS             1465..>2216
                     /gene="uspE"
                     /locus_tag="POB34_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HDI5921655.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLP"
BASE COUNT          580 a          538 c          540 g          558 t
ORIGIN      
        1 tatgagcgca tttctgccac caacccaggc ggtttaagcg acaagcttcg tgattatttt
       61 gccggtaatc ttagcattat tgatacgctt cccactgcca caggggggac gccatttcag
      121 cgcgaagtct ggaaaactct acgcactatc ccctgcgggc aggtaatgca ttacggccaa
      181 ctggctgaac aattgggccg ccctggcgcg gcgcgtgccg ttggtgcggc aaacggatcg
      241 aatcccatca gcatcgtcgt accttgccat cgggttattg gccgaaacgg caccatgacc
      301 ggatatgcag gcggagttca gcgaaaagag tggttattgc gccacgaagg ttatcttttg
      361 ctgtaaacat taaacaattt gtgccagctt gttcacactt ttatgtaaag ttacccttaa
      421 caacttaagg gttttcaaat agatagacat atatttacat ctaatatcgg aattctctgc
      481 tgttaaggtt tgcttagact tacttgctcc ctaaaaagat gttaaaattg acaaatatca
      541 attacggctt gagcagacct atgatcccgg aaaagcgaat tatacggcgc attcagtctg
      601 gcggttgtgc tatccattgc caggattgca gcatcagcca gctttgcatc ccgttcacac
      661 tcaacgaaca tgagcttgat cagcttgata atatcattga gcggaagaag cctattcaga
      721 aaggccagac gctgtttaag gctggtgatg aacttaaatc gctttatgcc atccgctccg
      781 gtacgattaa aagttatacc atcactgagc aaggcgacga gcaaatcact ggtttccatt
      841 tagctggcga cctggtggga tttgacgcca tcggcagcgg ccatcacccg agcttcgctc
      901 aggcgctgga aacctcgatg gtatgtgaaa tcccgttcga aacgctggac gatttgtccg
      961 gtaaaatgcc gaatctgcgt cagcagatga tgcgtctgat gagcggtgaa atcaaaggcg
     1021 atcaggacat gatcctgctg ttgtcgaaga aaaatgccga ggaacgtctg gctgcattca
     1081 tctacaacct gtcccgtcgt tttgcccaac gcggcttctc ccctcgtgaa ttccgcctga
     1141 cgatgactcg tggcgatatc ggtaactatc tgggcctgac ggtagaaacc atcagccgtc
     1201 tgctgggtcg cttccagaaa agcggcatgc tggcagtcaa aggtaaatac atcaccatcg
     1261 aaaataacga tgcgctggcc cagcttgctg gtcatacgcg taacgttgcc tgatttttcc
     1321 gcataactca ctatccttct gtcatatcat taaatttttc tgatttattg atctggcaga
     1381 aggttcatca ctgtttcatt caccagatat gggttaatct tttaattaca aactgcgttg
     1441 acagttgttg taaggagacc ctgtatggct atgtatcaga acatgctcgt tgttatcgat
     1501 cctaaccagg acgaccaacc agcattgcgg cgagctgttt atttacatca acggattggt
     1561 ggcaaaatta aagccttttt gccgatctat gacttctcat acgaaatgac caccctgctc
     1621 tccccggacg aacgtaccgc tatgcgtcag ggcgtcatca gccagcgtac agcctggatc
     1681 catgagcagg caaaatatta tctcaatgct ggcgttccca ttgaaattaa agtggtctgg
     1741 cataaccgtc ccttcgaagc catcattcag gaagtgatca gcggcgggca cgatttggtg
     1801 ctaaaaatgg cgcaccaaca tgaccgtctg gaagcggtga tttttacgcc aacggactgg
     1861 catctgttac gcaaatgccc aagcccggtg tggatggtga aagaccagcc gtggccggaa
     1921 ggaggcaagg cgctggtggc ggtgaatctc gccagtgaag agccgtatca taatgcgctc
     1981 aatgaaaaac tggtcaaaga gacgatcgaa ctggcagaac aagtcaacca taccgaagtt
     2041 catctggttg gcgcttatcc ggtaacgcca attaatatcg cgattgaact gccggaattt
     2101 gatccgagcg tttataacga tgccattcgt gggcaacatt tgctggcaat gaaagctctg
     2161 cggcagaaat tcggcattaa tgaaaacatg acgcacgtag aaaaaggtct gccaga
//
LOCUS       DAMZET010000329         2192 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.329, whole genome shotgun sequence.
ACCESSION   DAMZET010000329 DAMZET010000000
VERSION     DAMZET010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.329"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            1598..2192
                     /locus_tag="POB34_004839"
                     /pseudo
     CDS             1598..2192
                     /locus_tag="POB34_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279079.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          656 a          461 c          538 g          537 t
ORIGIN      
        1 tagtgcagct tcctcggcaa cggcggcagg aaattccgcg aaggccgcaa aaacgtctga
       61 gacgaatgcg gataacagcg cacaggcggc agcagactca caaactgcat cggcaaactc
      121 cgcgacagca gccaaaaaat cagaaaccaa cgcgaaaaat agcgaggcag cagcaaagag
      181 cagcgaaaca aacgctaaag ccagcgaaac taatgctaaa tccagtgaaa caaacgcggc
      241 gaaatctgcg gcggacgcac ttaattattg caaccaggcg caagtaattg ttggcgataa
      301 tatcggtctt ggctcagccc cgcgtgattg tcctgatatt tccggcaacc catcagggta
      361 tatcggattt atgcgcatta tgagtaatgc aaaaggcttt ccatcgattg catccggtga
      421 aagcagtctt acggggttta ttagtcaggt agatggaaca ccagcgtata caggtgtatt
      481 tcagggatgg gctacgcgct cgctttatac ttatcgctgg aatccgacaa caggcccgca
      541 atggacacgc cacgctcgca aaaatgaagt ggaccgtctt gaccaatgga atagcgaaac
      601 atggttatat aaccatgata aatccatgcg cctgggttta accggatcaa catgggggtg
      661 ttacagcgac acacaaaaaa aatggatacc gcttgatgtt tctcatggtg gcactggcgc
      721 aaatagcctt gatgacgcca aaactaattt gcaaatccct gagggtggat taacaaaagc
      781 gatgaccctt aatgcccctg gtggtgcagt ggatggcaaa tactaccctg tcataataga
      841 cacatctgca atgaatggga gcggtgccat gttatgtcct gttgaaataa aaacagcagg
      901 aggtccggca tcaaatccgc ttaatagtaa ctcatttttt gggttcattc gatgtggtgg
      961 ctggagtgac gttcgcgatg tggcttacgg gtcatttgtt tgctatgaaa aaaatgaact
     1021 cgggatactt tgcattaaag cgtcgcaaag agattatgcg cagcatgtag ctttctacat
     1081 ccataaaagc gcttttcctg tcgttgtgca aactggctac aaggcgaaag ttaaggttcc
     1141 aacagaagac tacgtaattg gtgacaacgg ggttagatat aagtttggtg tatcaacgtc
     1201 aacggaggat aacgacaaga actttgttaa aaatgttctt gacttcactg gcggtgaaca
     1261 tggcttctac agcaaccttc cgttcagaca aggggtgtca gaaaggattg cattaacaaa
     1321 tggcctgagt tttgacaaaa attttaccgt cactattcct atatttgtaa acaatggtca
     1381 gattaaagca acacaatctt tcaccgcgta cggtacagac gcttcaaaca gaacatttgt
     1441 ttctcaaaga ttacaatccg aaggaggtcc ggttgtcgat caaactgaac taagggccgc
     1501 taacgcttct ggtgaaatta ttgtcaggga cataaataat tcagggaaca gtaagttctt
     1561 taattttaac cttgatggta ccttcagttc ttaccaggga ttggttgttc acacagggca
     1621 aaactggagc actcaacata cagataatgt aaataagttt aagccaatag cagggagcgc
     1681 aggcggtcct gacggaacaa tggttgttgg cggattccat gctcaattta gcggtaatta
     1741 cgtcacacaa ttcgccggtc gcaactccaa attttgggca agaagctttg aggctggggt
     1801 tgataaggga tggaaacgac tattaacagt agacgatctc aattcatcta ccgatcttgc
     1861 tgtcaggtca ttaaccacat ctaacccggt aaaatctggc ggagggcgaa ttgatgtcct
     1921 tggaagcacg tcagactata gcaaaatgga ttgctttgta cgtgggtttg atagcaccgg
     1981 taattctctc gcgtgggcgt tgggttcgtc agtcggcgta agtaagatgc tgtcgctaaa
     2041 aaatttcttt agcggagctg agatactgtt aaatggtaat gacggcgcgg ttcaactcaa
     2101 aacaggtgct gttaacgggg ctaaagcgca ggcgctcact atcaacaagg atgaggttaa
     2161 ttcaactgtt gatttaactc ttacaaaaca aa
//
LOCUS       DAMZET010000330         2175 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.330, whole genome shotgun sequence.
ACCESSION   DAMZET010000330 DAMZET010000000
VERSION     DAMZET010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.330"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..224)
                     /locus_tag="POB34_004840"
     CDS             complement(<1..224)
                     /locus_tag="POB34_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HDI5921656.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATE"
     gene            343..1308
                     /gene="ycjG"
                     /locus_tag="POB34_004841"
     CDS             343..1308
                     /gene="ycjG"
                     /locus_tag="POB34_004841"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HDI5921657.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADIDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(1283..2011)
                     /gene="mpaA"
                     /locus_tag="POB34_004842"
     CDS             complement(1283..2011)
                     /gene="mpaA"
                     /locus_tag="POB34_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HDI5921658.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCANPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
BASE COUNT          511 a          516 c          620 g          528 t
ORIGIN      
        1 atctcagttg ccagttggtt aaacttacgt actgatgcgg cgcaaacacc ggtatcaata
       61 ctcgggaaaa tgttcagcac tttgcgttta cccgcaaact gaccgagggt gacgtcagac
      121 agatcttttg ccacgagagt aaaagtctgc gctttgctac ccgcctgcgg gatggaattg
      181 gcgactgtaa ccgggttgcc ctggaaatga acggtttgtg acatgattat ctttcctgtt
      241 tacatatagt taacgtcaca cctagtttat gctaactgtc aataacacag caaacgctat
      301 ttgcgcttaa tctgcagacc accgcgacaa caaggagtaa agatgagaac cgttaaggta
      361 tttgaggaag cctggccctt acataccccg tttgtgattg cccggggaag tcgcagtgaa
      421 gcgcgcgtgg tggtggttga actggaagaa gagggtatta aaggcaccgg cgaatgcacg
      481 ccgtatccgc gttatggaga aagtgatgcc tcggtaatgg cgcaaattat gagcgtcgtg
      541 tcgcaattag agaaagggct gacacgggag gagttgcaaa aaattctccc tgccggcgca
      601 gcacgtaatg cgctggattg tgcattgtgg gatctggccg cgcgaaaaca gcagcaatcg
      661 ctggctgatt tgatcggcat aacgcttccc gagacagtta tcactgcaca gacggtagtc
      721 atcggtacgc ctgatcagat ggccaatagt gcatcaacac tctggcaggc aggcgcgaaa
      781 ttactgaaag tgaagctgga taaccatctt attagtgagc ggatggtggc aattcgcaca
      841 gctgtgcccg atgcgacgct gatcgttgat gcaaatgaat cctggcgtgc agaagggttg
      901 gcggcgcgtt gccagctatt ggcggattta ggcgttgcga tgcttgaaca accccttcct
      961 gcgcaggacg atgcggcgct ggagaatttt attcatccgt tgccgatttg tgctgatgag
     1021 agttgtcata cacgtagcaa tctgaaggcg ctgaaagggc gctatgagat ggttaacatt
     1081 aagctcgata aaaccggtgg tctgacggaa gcgctggcgc tggcgactga agcgcgtgca
     1141 caaggtttca gtctgatgct gggctgcatg ttgtgtacct ctcgtgccat tagcgccgct
     1201 ttaccgctgg tgccgcaggt cagtttcgcc gatattgacg gaccgacctg gctggcggta
     1261 gatgtggaac cggcgcttca gttcacgacg ggcgaattgc atctttagga tgccagcgca
     1321 gcaagtttgc catcgcaaag agatattttt cgctggcttc gtcggaggag atgggcggaa
     1381 actctgcggt gatgcaatgc aagttgagat ctgcacacca actgccgaaa gagccaggcg
     1441 tttcataacc gacgctggtt accagaggca attcaaacgc ctgggccagc cattcgccta
     1501 attcgctgtg tctgggatct tcaatacagg ccagtggatc gtggaatgag accacccagg
     1561 caggttgaat gcggtgtata agctggcaca gcgcctgggt ttcaggttct gagccaggtt
     1621 tgtcacctgt cagtaatacg acatcacgtt cttcagcggc gctgttccag cgataaaccg
     1681 tttcaccttc cttccagttc gccgccggaa agtttctgtt taaatccaca ccattagcat
     1741 tggcccgtaa ccccagctgg cagccgtcag gattcgcaca cagcaccaca tgatggcggc
     1801 gcaaagaagg tgtcaacgtc cgcagagcac aggagagcgt gacgaccgaa gagttttcat
     1861 caccgtgagt gccagccaga atcaaaccac tttcgcggct ggctgcaggg gcaggaaacc
     1921 agattaacgg cgcgcccaat aatgaacgtc cgtaatgttc tgttccgggc ggaaatgcgc
     1981 cgcgttcggc gcgtgggcgg gttacggtca tgatcgtctc tggttcagga agtattacag
     2041 gcagtgttgt gcaaattttg cggattatca aatccttttc ttcaacgcta tgtcatcgat
     2101 cttgtatcac tttatttgcc ggaagttgtt tgcattttat tagttggata gggtttgtgg
     2161 atttttgctg gctga
//
LOCUS       DAMZET010000331         2173 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.331, whole genome shotgun sequence.
ACCESSION   DAMZET010000331 DAMZET010000000
VERSION     DAMZET010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.331"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..237
                     /locus_tag="POB34_004843"
     CDS             <1..237
                     /locus_tag="POB34_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921659.1"
                     /translation="GLEFDVIAAVVVGGTALSGGRGSLFGTLLGVLVITLIGNGLVLL
                     GINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            270..1364
                     /locus_tag="POB34_004844"
     CDS             270..1364
                     /locus_tag="POB34_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDI5921660.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            1376..>2173
                     /locus_tag="POB34_004845"
     CDS             1376..>2173
                     /locus_tag="POB34_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HDI5921661.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPE"
BASE COUNT          453 a          532 c          676 g          512 t
ORIGIN      
        1 ggtctggagt ttgacgtcat cgccgcggtc gtggtcggcg gtacggcact ttccggcggt
       61 cgcggttccc tgttcggtac attgcttggt gtgctggtga ttacgctaat cggtaacggt
      121 ctggtgctgc tcggtattaa ctcctttttc cagcaggtgg tacgcggcgt catcatcgtg
      181 gtggcggtgc tggcgaatat cttgctgacc cagcgaagca gtaaagcgaa acgctaaacc
      241 ttaacgcccc tggcgcgggt gcggcaggga tgaaaattaa tcgtattgtt aatgagggat
      301 ttatgaaaac gatgctggca gcttatttac caggaaattc gaccgtcgat ctacgggaag
      361 ttgcggtgcc gacaccaggg attaaccagg tactgatcaa aatgaaatct tccggaattt
      421 gcggaagcga tgtccactat atctaccacc agcaccgcgc tacggcagcg gcacccgaca
      481 aaccgttata ccagggattt atcaacggtc atgaaccgtg cgggcagatt gtggcgatgg
      541 ggcaaggctg ccgccatttt aaagagggcg accgcgtgct ggtgtatcac atttctggct
      601 gtggtttttg cccgaactgc cgccgcggct ttcctatctc ttgtactggc aaaggaaaag
      661 cggcttacgg ctggcagcgt gacggcggtc atgccgaata tctgctggcg gaagaaaaag
      721 atctgatcct cctgccggat gcgctgagct acgaagatgg tgcgtttatc agttgcggcg
      781 ttggcacggc ctatgaaggg attttgcgcg gcgaagtttc cggcagcgat aacgtgctgg
      841 tggtcggtct ggggccggtc ggcatgatgg cgatgatgct ggcgaaaggt cgcggtgcaa
      901 aacggatcat cggcgttgat atgctgccgg aacgtctggc gatggcaaaa cagttagggg
      961 tgatggatca cggctattta gcaaccaccg aaggtctgcc gcagattatc gccgaactca
     1021 cccacggtgg cgcggatgtt gcgctcgatt gttccggtaa tgccgcaggt cgcttgctgg
     1081 cactgcaatc caccgctgac tggggacggg tggtttacat tggtgaaacc ggaaaagtgg
     1141 aattcgaggt cagcgccgat ctgatgcacc atcaacggcg gattatcggc tcctgggtga
     1201 ccagtctgtt ccatatggaa aaatgcgccc atgatctgac ggactggaag ctgtggccgc
     1261 gtaacgccat tacccatcgc ttctcgctgg aacaggcagg tgatgcctat gcgctgatgg
     1321 cgagcggcaa atgcgggaaa gttgtgatta acttcccgga ttaaggagtt agcggatgac
     1381 catctatacc ttatcccacg ggtcgctaaa gctggacgtt tctgaccagg gcggcgtaat
     1441 cgaaggcttc tggcgcgata cgacgccgct cttacgtcct ggtaaaaaaa gcggtgtggc
     1501 aaccgatgcc tcatgttttc cgctggtgcc atttgcaaac cgggtgagcg gcaatcgttt
     1561 tgtctggcag gggcgtgagt atcaactgca accgaatgtt gagtgggatg cgcactatct
     1621 acacggcgat ggctggctgg gcgagtggca atgtgtctcg cacagcgatg atagtttgtg
     1681 tctggtgtat gaacatcgca gcggtgtcta tcactatcgg gtaagtcagg cgtttcattt
     1741 aacggcggat acgctgacgg tgacgctctc tgtcaccaat caaggggcag agacgctgcc
     1801 atttggtacc ggctggcatc cttattttcc gttgtcgccg caaacgcgga ttcaggcaca
     1861 ggcgagcggt tactggctgg agcgggagca gtggctggcg ggtgagtttt gcgagcagct
     1921 accgcaggaa ctggatttta accagcccgc gccgttgccg cgccagtggg tgaacaatgg
     1981 ttttgccgga tggaatggtc aggcccgcat cgagcagccg caggaaggtt atgccatcat
     2041 catggaaacg acgccacctg caccgtgtta tttcatcttt gtttccgacc ctgcgtttga
     2101 taaaggatat gcgtttgatt tcttctgtct ggaaccgatg agccatgcgc cggatgatca
     2161 tcatcgcccg gaa
//
LOCUS       DAMZET010000332         2171 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.332, whole genome shotgun sequence.
ACCESSION   DAMZET010000332 DAMZET010000000
VERSION     DAMZET010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.332"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            87..269
                     /gene="ycaR"
                     /locus_tag="POB34_004846"
     CDS             87..269
                     /gene="ycaR"
                     /locus_tag="POB34_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HDI5921662.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            266..1012
                     /gene="kdsB"
                     /locus_tag="POB34_004847"
     CDS             266..1012
                     /gene="kdsB"
                     /locus_tag="POB34_004847"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDI5921663.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIRNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAHEVPGTGVDTPEDLERVRAEMR"
     gene            1166..2059
                     /locus_tag="POB34_004848"
     CDS             1166..2059
                     /locus_tag="POB34_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HDI5921664.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(2036..>2171)
                     /locus_tag="POB34_004849"
     CDS             complement(2036..>2171)
                     /locus_tag="POB34_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HDI5921665.1"
                     /translation="SPLNPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE
                     "
BASE COUNT          519 a          524 c          590 g          538 t
ORIGIN      
        1 gccgcactgg ctgggaaata gaccccgtcg cataaatgat tatgataaga tttatgcatt
       61 cataacccga tccatctgga ggaactatgg atcatcgttt gcttgaaatc attgcctgcc
      121 cggtttgcaa cggaaaactt tggtataacc aggaaaaaca agagcttatt tgcaaactgg
      181 ataacctggc tttcccgctc cgcgatggca ttccagtgtt gctggaaacg gaagcccgcg
      241 tgctgactgc tgatgagagt aaatcatgag ttttgtggtc attattcccg cgcgctacgc
      301 gtccacgcgt ctgcccggta aaccattggt tgatattaac ggcaaaccca tgattgttca
      361 tgttcttgaa cgcgcgcgtg aatcaggtgc cgagcgcatc atcgtggcaa ccgatcatga
      421 ggatgttgcc cgcgccgttg aagccgctgg cggtgaagta tgtatgacgc gcgccgatca
      481 tcagtcagga acagaacgtc tggcggaagt tgtcgaaaaa tgcgcattca gcgacgacac
      541 ggtgatcgtt aatgtgcagg gtgatgaacc gatgatccct gcgacaatca ttcgtcaggt
      601 tgctgataac ctcgctcagc gtcaggtggg tatggcgact ctggcggtgc caatccgcaa
      661 tgcggaagaa gcgtttaacc cgaatgcggt gaaagtggtt ctcgacgctg aagggtatgc
      721 actgtacttc tcacgcgcca ccattccttg ggatcgtgat cgttttgcag aaggccttga
      781 aaccgttggc gataacttcc tgcgtcatct tggtatttat ggctaccgtg caggctttat
      841 ccgtcgttac gtcaactggc aggcaagtcc gttagaacac atcgaaatgt tagagcagct
      901 tcgtgttctg tggtacggcg aaaaaatcca tgttgctgtt gcgcatgaag ttcctggcac
      961 aggtgtggat acccctgaag atctcgagcg cgttcgcgct gaaatgcgct aatttcactt
     1021 cacgacactt cagccaattt tgggaggagt gtcgtaccgt tacgattttc ctcaattttt
     1081 cttttcaaca attgatctca ttcaggtgac atcttttata ttggcgctca ttatgaaagc
     1141 agtagctttt atgagggtaa tctgaatgga acagctgcgt gccgaattaa gccatttact
     1201 gggcgaaaaa ctcagtcgta ttgagtgcgt caatgaaaaa gcggatacgg cgttgtgggc
     1261 tttgtatgac agccagggaa acccaatgcc gttaatggca agaagcttta gtacgcccgg
     1321 aaaagcccga caactggcat ggaaaaccac catgctggca agaagtggga ctgtccgtat
     1381 gccgactatt tatggtgtga tgacgcatga agaacacccc ggccccgatg tcctgctact
     1441 ggagcggatg cgtggtgttt cggtggaggc accagcccga acaccagaac gctgggaaca
     1501 actcaaagac caaatcgttg aagccttact ggcctggcac cgtcaggaca gtcgcggttg
     1561 cgtcggcgcg gtcgacaata ctcaggaaaa tttctggccc tcatggtacc ggcaacatgt
     1621 tgaagtgcta tggaccacgc tcaatcagtt caataacacc ggtctgacga tgcaggataa
     1681 gcggatcctg tttcgcactc gcgaatgtct cccggcatta tttgaaggct ttaacgacaa
     1741 ttgtgtgttg attcacggta acttctgttt acgcagcatg ctgaaagatt cgcgcagcga
     1801 tcagttactg gcgatggtcg ggccgggact aatgctttgg gcaccgcgag aatacgaact
     1861 gttccgacta atggataatt ctctggcgga agatttgctc tggagttacc tgcaacgcgc
     1921 gccagtggcg gagtcgttca tctggcggcg ttggttgtat gtgttatggg atgaagttgc
     1981 gcaactggtt aataccggac gatttagtcg gcgcaacttc gatctggcat caaaatcact
     2041 cttgccgtgg ctcgcctgac ggacctttca gccactgcca gatgcggcca agcgtttcat
     2101 agccaacacg atcgctatgc atcagccaga ccggagaagg gattgcccgt tcccacggat
     2161 taagcggcga g
//
LOCUS       DAMZET010000333         2150 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.333, whole genome shotgun sequence.
ACCESSION   DAMZET010000333 DAMZET010000000
VERSION     DAMZET010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.333"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..569)
                     /gene="dadA"
                     /locus_tag="POB34_004850"
     CDS             complement(<1..569)
                     /gene="dadA"
                     /locus_tag="POB34_004850"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HDI5921666.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGL"
     gene            899..>2150
                     /locus_tag="POB34_004851"
     CDS             899..>2150
                     /locus_tag="POB34_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HDI5921667.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIH"
BASE COUNT          544 a          558 c          527 g          521 t
ORIGIN      
        1 agaccacccg tcagtttgtg cgctacttct gccagcgcgg gctccacttc cgccaggcgg
       61 ctggattcca gcagctgata cggtacgccg gcatcttcca gcacggcgat atcgcgggtc
      121 gcattttcat actgttgttc ggtacggaac agttgcagcg tcccaccctg acgcccttca
      181 tactgaatat tggtttcggc gcgcaatgct ttcaggcaat cacggctgta ttccgccaga
      241 cgcaccatcc gccctttgtt ttccatgtag tggctggtgt cgcagttacg taacatttgc
      301 cacatccatt tcaactggaa ctgcgtaccg tcgagacgaa ccgccagcgg cgcatggcgc
      361 tggaacatcc atttaatcgc ttttaaaggc acacctggtg ccgcccacgg tgcagcatat
      421 ccgggggaga tttgcccggc attcgcggcg ctggtttcca gggcggcccc cggctcccga
      481 tcaatgacgg tgacctcatg tcctgcctga tttaagtacc aggcgctggc aacgcctacc
      541 acaccacttc ccagtatgac aactcgcata gccactccga taacggtaga tacagtaaaa
      601 gaataataat ctaattacat cttgataact cagttgaaaa tattattcaa catacggctt
      661 ttttatggtg agctggctca catctccctg actctgcggc aatatggcgt ctttagtatc
      721 tcctgctgaa cgcccttttt atccagcata aaattccgtt cagaagcgga ttagtggcac
      781 tctgataata agaaatcgca aagaaaaaaa ttctccggcg acgcggtttt taacaggtgt
      841 tctatgcttg aaatgaggtg ctccacacag agagcgccga caacaatgag gtgcgcgtat
      901 ggcgacgatc gattctatga ataaggacac cacacgtttg agcgatggac ccgactggac
      961 gttcgacctg ctggatgttt atctggcaga gatagaccgg gtggcgaaac tctaccggct
     1021 ggatacctac ccgcaccaga ttgaagtgat aacctcagaa cagatgatgg atgcctactc
     1081 cagcgtcggc atgccaatta actatccgca ctggtcattc ggtaaaaagt ttatcgagac
     1141 tgaacggctg tataagcacg gtcagcaagg actggcctat gaaatcgtca ttaactctaa
     1201 cccgtgtatc gcttacctga tggaagagaa caccattacc atgcaggcgc tggtgatggc
     1261 acacgcctgc tatgggcata actctttctt taaaaacaat tacttattcc gcagttggac
     1321 cgacgccagt tcgattgtcg attatctgat ttttgcccgt aaatatatta ccgagtgcga
     1381 agaacgttat ggcgttgatg aagtagaacg gcttctggac tcgtgccacg cgctgatgaa
     1441 ctacggcgtg gaccggtaca aacgcccgca aaaaatctcg ctgcaagaag agaaagcccg
     1501 gcagaaaagt cgcgaagagt atctacaaag tcaggtcaat atgctctggc gtaccctgcc
     1561 gaagcgcgag gaagagaaaa cggttgctga agcgcgccgc tatccgtccg aaccacaaga
     1621 aaacctgctc tattttatgg agaaaaatgc gccactgctg gaatcatggc agcgtgaaat
     1681 cctgcgtatt gtgcgtaagg tgagccagta tttttatccg caaaaacaga ctcaggtgat
     1741 gaacgaaggc tgggcgacct tctggcacta taccatcctt aaccatctgt atgatgaagg
     1801 gaaagtaacg gaacgtttta tgctggagtt tttgcacagc cacaccaatg tggtcttcca
     1861 gcccccctat aacagcccgt ggtacagcgg catcaacccg tatgccctcg ggttcgccat
     1921 gttccaggat attaaacgga tttgtcagtc gccaacggaa gaagacaaat actggttccc
     1981 ggatatcgcc ggttccgact ggctggaaac gctgcatttc gcgatgcgtg atttcaaaga
     2041 tgagagtttt atcagccagt ttctgtcacc gaaagtgatg cgtgatttcc gcttcttcac
     2101 cgtgctggat gatgatcggc ataattatct ggagatttcc gctattcata
//
LOCUS       DAMZET010000334         2144 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.334, whole genome shotgun sequence.
ACCESSION   DAMZET010000334 DAMZET010000000
VERSION     DAMZET010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.334"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..278
                     /locus_tag="POB34_004852"
     CDS             <1..278
                     /locus_tag="POB34_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDI5921668.1"
                     /translation="GLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGI
                     GASGNHRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            275..1618
                     /gene="astB"
                     /locus_tag="POB34_004853"
     CDS             275..1618
                     /gene="astB"
                     /locus_tag="POB34_004853"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HDI5921669.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            1611..>2144
                     /locus_tag="POB34_004854"
     CDS             1611..>2144
                     /locus_tag="POB34_004854"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HDI5921670.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGA"
BASE COUNT          455 a          548 c          648 g          493 t
ORIGIN      
        1 tcggactctc ttgcggtctg gtttcccccg agcgggaaaa attcgatcaa ctgttgctgg
       61 aggcgcgggc ggggattgtt aactggaaca aaccgcttac tggtgctgcc agtaccgcgc
      121 cattcggcgg cattggtgca tccggtaacc atcgccccag cgcctggtat gccgcagatt
      181 actgcgcatg gcctatggcg agcctggagt cggactcgtt aactttgccc gcaacgctta
      241 accccgggct ggatttttcc gatgaggtgg tgcgatgaac gcctgggaag ttaatttcga
      301 cgggctggta gggctgacgc atcattacgc tggcctgtcg tttggtaatg aagcctctac
      361 ccgtcaccgt tttcaggtgt ctaacccgcg acaggcggca aaacagggct tactgaaaat
      421 gaaagccctt gccgatgcgg gattccccca ggccgtgatc ccgccgcacg agcgtccgtt
      481 tattccggtg ctgcgtcagt tgggattcag tggtagcgat gagcaggtac tggaaaaagt
      541 tgcacgccag gcaccgcact ggctttccag cgtcagttcc gcttcgccaa tgtgggtagc
      601 caatgcggca acgatcgcgc catctgccga tacgctggat ggcaaagtgc atctcaccgt
      661 tgccaacctg aacaataaat ttcaccgctc gctggaagcg cacgtcaccg aatcactttt
      721 aaaagcgatt tttaacgacg aagagaaatt tagcgttcat tcggcgttgc cgcaggtagc
      781 gttgctcggt gatgaggggg cggcaaacca caatcgtctc ggcggtcatt acggtgagcc
      841 gggtatgcaa ctttttgtct acgggcgaga agaaggcaat gatacccggc cttcccgtta
      901 tccggcgcga cagactcgcg aagccagcga ggcggtggca aggctgaatc aggtgaatcc
      961 ccaacaggtg attttcgccc agcaaaaccc ggacgttatc gaccagggcg tttttcataa
     1021 tgacgtgatt gccgtgagta accgccaggt gctgttttgc caccaacagg cgttcgctcg
     1081 ccagtcacag ttactggcaa acctgcgtgc gcgggtgaac ggttttatgg cgatagaagt
     1141 tccggcaact caggtttccg tgtcggatgc ggtgtctacc tatctgttta acagccaact
     1201 gttgagccgc gatgatggtt ccatgatgtt ggtgctgcct caggagtgtc gggaacacgc
     1261 cggagtatgg ggttatctca atgaactcct tgccgctgac aacccgatta gcgaactaaa
     1321 agtctttgat ttacgtgaaa gcatggcgaa tggcggcggc ccggcgtgcc tgcggttgcg
     1381 ggtggtattg acagaagaag aacgccgggc ggtgaatccg gcggtgatga tgaacgatac
     1441 gctgtttaat gcgctcaatg actgggtgga tcgttactac cgcgatcgcc ttactgctgc
     1501 cgatctggcc gacccgcaat tgctgcgcga agggcgggaa gcactggatg tattgagcca
     1561 attactgaat ctcggttcgg tttatccgtt ccagcgcgag ggagggggca atggataatt
     1621 ttcttgctct gaccttaacg ggtaaaaaac cggttatcac cgagcgagaa atcaacggcg
     1681 ttcgctggcg ctggctgggc gatggtgtgc tggaactgac gccattaacg ccaccgcaag
     1741 gcgcactggt gatttcagcg ggaatacacg gtaatgagac ggcacctgtg gagatgctgg
     1801 acgcgttgct tggcgcgata tctcacggcg agatcccgtt acgttggcgg ttgctggtga
     1861 tcctcgggaa tcctcctgcg ctgaagcaag ggaaacgtta ttgccatagc gatatgaatc
     1921 gaatgtttgg cggtcgttgg cagctatttg ctgaaagcgg agaaacctgt cgggcgcgcg
     1981 aactggaaca gtgcctggaa gatttttatg accagggcaa agaatctgtg cgctggcacc
     2041 ttgatctaca taccgcaatt cgtggctcct tgcatccgca gttcggtgta ttaccgcaac
     2101 gcgacattcc ctgggacgag aaatttctga cgtggctggg tgcg
//
LOCUS       DAMZET010000335         2134 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.335, whole genome shotgun sequence.
ACCESSION   DAMZET010000335 DAMZET010000000
VERSION     DAMZET010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.335"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..941
                     /locus_tag="POB34_004855"
     CDS             <1..941
                     /locus_tag="POB34_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDI5921671.1"
                     /translation="TGSWMIGMAGVVIHAAFVYKLGDWFARDTRNFFELEGIAIPHGT
                     SAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKFGPFGEPVTVGFVMGLIIGILAGY
                     DVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAKQARSRLQAKFGGQEFLIGLDPAL
                     LLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGDLATIGFFVAMAVAVHRGNLFRTL
                     ISGVIIMSITLWIATQTIGLHTQLAANAGALKAGGMVASMDQGGSPITWLLIQVFSPQ
                     NIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLAE"
     gene            989..2029
                     /gene="gatD"
                     /locus_tag="POB34_004856"
     CDS             989..2029
                     /gene="gatD"
                     /locus_tag="POB34_004856"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDI5921672.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAKGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
BASE COUNT          482 a          469 c          592 g          591 t
ORIGIN      
        1 caaccggttc atggatgata gggatggcag gtgtcgtaat tcacgcggcg tttgtttata
       61 agctcggcga ctggtttgcc cgcgataccc gaaatttctt tgagctggaa ggtattgcta
      121 ttccgcacgg tacgtcggcg tatatggggc cgattgcggt gctggtcgat gctatcatcg
      181 agaaaatccc aggcgttaac cgaattaaat ttagcgccga cgatattcag cgcaaatttg
      241 gtccatttgg cgagcctgtc accgtgggtt ttgtgatggg gctgattatc ggcatcctcg
      301 cgggttacga tgtcaaaggt gtattgcagc tggcggtaaa aacggcggca gtgatgctgc
      361 taatgccacg ggtgattaaa cccatcatgg atggtttaac gcccatcgct aagcaggctc
      421 gtagtcgttt acaggcgaag ttcggcggtc aggagttcct gattggcctt gatccggcgt
      481 tgctgctggg acatacggcg gtggtatcgg caagcctgat ttttatccca ctcaccattt
      541 taattgctgt ttgtgtgccg ggtaatcagg tgctgccgtt tggcgatctt gccaccatcg
      601 gcttcttcgt ggcgatggcg gtcgccgtgc atcgtggaaa tctgttccgc accttaatct
      661 cgggtgtcat catcatgagc atcactctgt ggatcgcgac gcaaactatt ggtttgcaca
      721 cccaactggc ggctaatgct ggggcgttaa aagccggggg tatggtggct tcaatggatc
      781 agggcggttc tcccattacc tggttactga ttcaggtttt ctccccgcaa aatattcccg
      841 gtttcattat tatcggtgca atttatctga ccggtatttt catgacctgg cgtagagcgc
      901 gtggctttat taaacaagag aaagtcgttc tcgcagaata atttttacct gagggggagg
      961 ttatccccct taacaatcag gagtttttat gaaatcagtg gtgaatgata ctgatggtat
     1021 cgtgcgcgtt gcagaaagcg tcattcctga aattaaacat caggatgagg tgcgggtaaa
     1081 aattgccagc tcgggcttat gtggttccga tttacccagg atatttaaaa atggtgcaca
     1141 ttattatcca ataacgttag gccatgaatt tagcggctat attgatgcgg tgggatccgg
     1201 tgttgatgat ttacaccctg gcgatgcggt tgcctgtgtg ccgttattac cctgttttac
     1261 ttgtccagag tgtctgaaag ggttttattc ccagtgcgca aaatatgatt ttattggctc
     1321 gcggcgtgat ggtggatttg ctgaatatat tgtcgttaag cgaaaaaatg tctttgctct
     1381 acccacggat atgcctattg aggatggggc ttttattgag ccgattaccg ttggtctgca
     1441 tgcttttcat ttagcgaaag gttgtgagaa taaaaacgtt attattattg gtgccggaac
     1501 cattggcctg ctggctattc agtgcgctgt cgcgctggga gcaaagagtg tgacggctat
     1561 cgacattagc tcagaaaaac tggcactggc aaaatctttc ggtgcgatgc aaacatttaa
     1621 cagtagcgaa atgagcgcgc cgcaaatgca gagcgtttta cgcgaactgc gttttaatca
     1681 gcttatcctc gagacggctg gcgtaccgca aactgtcgaa ctggcggtag agattgccgg
     1741 tcctcatgcc caactggcgc tggtgggcac gttgcatcag gatctgcatt taacatcggc
     1801 aacgtttggc aaaatattgc gtaaagagct gacggttatc ggcagttgga tgaactattc
     1861 cagcccttgg ccggggcagg agtgggaaac ggcgagccgg ttgctgacag aacgtaagtt
     1921 aagcctggag ccattaatcg ctcaccgtgg aagctttgaa agcttcaccc aggcggtgcg
     1981 tgacatcgct cgtaatgcca tgccgggcaa agtgttgctc attccatgaa accgcgggcc
     2041 agcgtgatgc tggcccggta ttgtgcaaaa cagatcattc accaatggtc ccccttcgtt
     2101 tacactagcc acaattgaat atgggtaaat gacg
//
LOCUS       DAMZET010000336         2131 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.336, whole genome shotgun sequence.
ACCESSION   DAMZET010000336 DAMZET010000000
VERSION     DAMZET010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.336"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1987
                     /locus_tag="POB34_004857"
     CDS             <1..1987
                     /locus_tag="POB34_004857"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HDI5921673.1"
                     /translation="GSNIYELLSDRTNIVVIADEAHRSQYGFSAKEVDVKDSEGNVTG
                     KRTVYGFAKYMRDALPNATYLGFTGTPIEKTDVNTPAVFGNYVDIYDISQAVEDGATV
                     RIFYESRLAKIAISDEGRQLIEDFDDEFNEDELTLTQKERSKWARIEGLIGSSKRIKA
                     IAADMVLHFEQRLKSNADHGKGMIVTMSRRIAAELYKEIIALKPEWHSDDLNDGIIKV
                     VMTSSAADGPEIAKHHTTKKERQVLANRMKDDDDKLKLVIVRDMWLTGFDAPSMHTLY
                     IDKPMKGHNLMQAIARVNRVYKDKIGGLVVDYLGIASDLKEALSFYSDAGGRGDPAEV
                     QEEAVTLMQEKLEILEGMMHGYDYKAYFAATTSQRLTIILESENHILGLDNGKGKMRF
                     LAAVAALSQAFALATPHDKAMEAAPEVAFFQAVKARLNKFTENSDGSEEEHNDSLEVR
                     VKQTIDQALVTDKVVDIFDAAGIQKPDISVLSEEFLQEMKDYQHRNIALETLKKLLSD
                     EIKVRSNQSITQGKKLIDMLTSAINGYQNKVLTAAEVIDELIKLAKTIQESDSLASQL
                     NLSAYEYAFYSAVADNDSARELMEKEKLRELAVVLTEAIRNNVSLDWTVKEAARAKIR
                     VVVKRLLKKYGYPPDMSLLATETVLKQAELLAGELGK"
     gene            complement(2033..>2131)
                     /locus_tag="POB34_004858"
     CDS             complement(2033..>2131)
                     /locus_tag="POB34_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HDI5921674.1"
                     /translation="LARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          646 a          460 c          491 g          534 t
ORIGIN      
        1 tggcagcaat atctatgagt tgttgtcaga cagaaccaat attgtcgtta tcgctgatga
       61 agcacaccgt tcccagtacg gtttcagcgc caaagaagtt gacgtgaaag acagcgaagg
      121 caacgtaaca ggtaaacgca ccgtttacgg ctttgccaaa tatatgcgtg atgccttacc
      181 taatgcgacc tatctcggct ttaccggaac ccccatagaa aaaacggacg tcaacacgcc
      241 tgctgttttt ggtaactatg ttgatatcta cgatatctcg caggccgttg aagatggtgc
      301 aacagttcgt atcttttatg aaagccgtct tgccaaaatt gccatcagtg atgaaggtcg
      361 tcagcttatt gaagactttg atgatgagtt taacgaggac gaactgacgc tcacgcaaaa
      421 agaacgttct aaatgggcca gaatcgaagg tttgattggc agttcaaaac gtattaaagc
      481 gattgcggcg gatatggttc tgcactttga gcagcgctta aaatccaatg ccgatcatgg
      541 taagggcatg attgttacca tgtcccgccg tattgctgct gaactatata aagaaatcat
      601 agccttaaaa cctgaatggc acagcgatga tttaaatgac ggtataataa aagtcgtcat
      661 gacctcttct gctgctgacg ggccagaaat tgccaaacac cacaccacaa aaaaagaacg
      721 tcaggttctg gctaaccgta tgaaggatga cgacgacaag ctgaaactgg tgatagtgcg
      781 tgatatgtgg ttaaccggct tcgacgctcc cagcatgcat acgctgtata tcgacaaacc
      841 aatgaaaggc cacaacctta tgcaggcaat tgcccgtgtg aaccgtgtgt ataaagataa
      901 gataggcggt ctggttgttg actatctggg cattgcctct gatttaaaag aagccctctc
      961 cttttactct gatgcaggtg gacgtggaga tcctgctgag gttcaggaag aagccgtaac
     1021 gctcatgcag gaaaagctgg aaatcctgga aggcatgatg catggatacg attacaaagc
     1081 ttactttgcc gcaactacct cacaacgcct gacaattatt cttgaatcag aaaaccatat
     1141 tttagggctg gataacggta aaggtaaaat gcgtttcctc gctgcggttg cagccttatc
     1201 gcaggcattt gcattggcga caccgcacga taaagcaatg gaagcggcac ccgaagtagc
     1261 attcttccag gcagtaaaag ccagactgaa taaatttact gaaaactcag acggatcaga
     1321 agaagaacac aatgacagtc tcgaagttcg ggtaaaacag actatcgatc aggctctggt
     1381 taccgataaa gttgttgata tttttgacgc tgctgggata caaaaacctg atatttccgt
     1441 tctttctgaa gaattccttc aggaaatgaa ggattaccaa cacagaaata ttgctttgga
     1501 aacgcttaaa aaactgcttt ctgacgagat caaggttcgc tcgaatcaaa gtatcaccca
     1561 aggcaaaaaa ctgattgata tgctgacctc tgcaatcaat ggctaccaga acaaggtact
     1621 gaccgcagcg gaggtaattg atgagctaat caagcttgcc aagactatcc aggaatctga
     1681 cagccttgcc agccagttaa acctcagcgc ttatgaatat gccttctatt ctgctgttgc
     1741 agataacgac agtgctcgcg agttaatgga aaaggaaaaa ctacgagaac tggcagtcgt
     1801 acttacggag gctatccgca acaatgtcag tcttgactgg acagtgaaag aagcagcaag
     1861 agcaaaaatt cgcgtggtgg taaaacgtct gctcaaaaaa tatggttatc cgcctgatat
     1921 gtcattgctc gccacagaga ctgttttgaa gcaggctgaa cttttagctg gagaattagg
     1981 aaaatgattc cctaaatttt tagcatataa actatttggt aatggtccca acttattgat
     2041 agtgttttat gttcagataa tgcccgatta ctttgtcatg cagctccacc gattttgaga
     2101 acgacagcga cttccgtccc agccgtgcca g
//
LOCUS       DAMZET010000337         2107 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.337, whole genome shotgun sequence.
ACCESSION   DAMZET010000337 DAMZET010000000
VERSION     DAMZET010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.337"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            1..2107
                     /locus_tag="POB34_004859"
                     /pseudo
     CDS             1..2107
                     /locus_tag="POB34_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          541 a          595 c          655 g          316 t
ORIGIN      
        1 accgggcgcg tgacggagca gtttaatccg gcaggcttaa gctacaccta tcagtatgag
       61 aaaaaccgca tcaccatcac tgacagcctg aaccggcgcg aggtgctgca cacggaaggt
      121 gaagccgggc tgaaacgtgt ggtgaaaacg gaactcgcgg atggcagcat cacccgaagt
      181 aaatttgact atatgggcga ctccagtcac agacggatgc agcaggccgg acaacagaat
      241 acagtccgaa tgtggtgacg ggactcgtca cgtgcatcac cacgccggac ggcaggaaat
      301 cggaatttta ctataacaat caaaaccagt taacctcagc caccgggcct gacggactgg
      361 aaatgcgccg gaaatatgat gagtacgggc gtctgacgca ggaaacagca cgcaacggcg
      421 atgtcacccg ctacagttat gataatccgc acagtgaatt accctctgca actgaagatg
      481 cgacgggtag ccgcaagcag atgacgtgga gccgctacgg gcagttacag accttcaccg
      541 actgctcagg atatgaaacg cattacgaat acgaccgctt tggtcagatg atggcggtac
      601 accgtgagga aggcatcagc acgtacaata cctataatcc gcgcggccag ctggtcagtt
      661 ggaaggacac gcagggccgt gaaacgcagt atgaatacaa cgccgcaggc gacctgaccg
      721 ccgtcattac cccggacggc aaccggagtg agacactgta cgatgcgtgg ggaaaggccg
      781 tcagcaccac acagggtggt ctgacgcgca gcatggaata cgatgcagcg ggacgtgtca
      841 tcagcctgac caacgagaac ggtagccata cagaatttag ctgggatgtg cttgaccgcc
      901 tgatacagca gcgcggcttt gacgggcgga cgcagcgtta ccgttatgac ctgaccggga
      961 aactcacgca gagcgaggat gaggggctta tcaccctctg gcactacgac gaatcggacc
     1021 gtatcacaca ccgcatggtg aacggcgaac cggcagagca gtggcggtat aacgaccacg
     1081 gctggctgaa agaaatcagc cacctgagcg aaggtcaccg ggtggcggta cattacggct
     1141 ataaccgtaa gggacgtctc acaggcgagc gccagacggt acatcacccg gagacggggg
     1201 aactgctctg gcagcatgag acgaaacacg cttacaacga gcaggggctg gcaaaccgct
     1261 ttcaggcaga cagcctgccg ccagtggagt ggctgaccta cggcagcggt tacctggcgg
     1321 gaatgaaact gggcgacacg ccgctggtgg agtacacacg ggacagactg caccgggaaa
     1381 cggtgcgcag cttcggcaat aatgcatatg aactgaccag cacatacact cacgcaggcc
     1441 ggttacagag tcagcacctg aacagcctgg tgtatgaccg tgactatggc tggaatgaca
     1501 atggcgacct ggtgcgcatc agcggcccgc ggcagacgcg ggaatacggc tacagcgcca
     1561 cgggcaggct ggagagcgtg cgcacccttg catcagacct ggacatccgc atcccgtatg
     1621 cgaccgaccc ggcgggaaac cgcctgccgg acccggagct gcacccggac agcaccctca
     1681 cggcgtggcc ggataaccgc atcaccaaag acgcgcatta cctgtaccgt tacgatgaat
     1741 acggcaggct gacggagaag acggaccgca tcccgagggg tgtgatacgg acggacgacg
     1801 agcggaccca ccactaccac tacgacagcc agcaccggct ggtgttttac acgcggatac
     1861 agcatgagga gccactggtc gagagccgct atctctacga cccgctgggc cgccggacgg
     1921 ggaaacgggt gtggcgtcgc gagcgtgatc tgacaggatg gatgtcgctg tcgcgtaaac
     1981 cggaggtgac gtggtacgga tgggacggcg acaggctgac gacggtacag accgacacca
     2041 cgcgtatcca gacggtatac cagccgggaa gcttcacgcc gctcatccgg gtcgagacag
     2101 agaacgg
//
LOCUS       DAMZET010000338         2107 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.338, whole genome shotgun sequence.
ACCESSION   DAMZET010000338 DAMZET010000000
VERSION     DAMZET010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.338"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(316..>2107)
                     /locus_tag="POB34_004860"
     CDS             complement(316..>2107)
                     /locus_tag="POB34_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI5921675.1"
                     /translation="AGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRR
                     KNNPLLVGESGVGKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFE
                     KRFKALLKQLEQDTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGST
                     TYQEFSNIFEKDRALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRA
                     AVELAVKYINDRHLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPE
                     KSVSQSDRDTLKNLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGP
                     TGVGKTEVTVQLSKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVI
                     KHPHAVLLLDEIEKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRET
                     ERKSIGLIHQDNSTDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQ
                     VQLDQKGVSLEVSQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDG
                     GQVTVALDKEKNELTYGFQSAQKHKAEAAH"
BASE COUNT          490 a          620 c          464 g          533 t
ORIGIN      
        1 cgagcttttt tttcgcattt tatctgtctg tacggcagtg aacgcgttcg caaaggtttt
       61 accgcacccc gtcgatttct aagctgcctg tacggcagtg aactcaaata ctacacgttt
      121 agcccgttgt taataaagca aaaccatccc attctcctgc agcacccttt tttatcctta
      181 ccttcccgca gcactaatta tcaacaactt accatcatca ttaaaaaaag ggtctgaacc
      241 tcctccaccc cataaacaaa aagtccgtaa cctctttcga gattacggac ttgaccaacc
      301 tacctaacaa tcagattaat gcgctgcttc cgccttgtgc ttttgtgcac tctggaatcc
      361 gtaagtcagc tcatttttct ctttatccag cgcgacggtg acctgaccgc cgtccaccag
      421 cgaaccaaac agcagttcgt tggcgagcgg ttttttcagg ttgtcctgga tgacacgcgc
      481 catcggacgt gcgcccattg cccggtcgta acctttctcg gccagccagt tacgcgcttc
      541 ctggctcact tccagagaaa cacctttctg atccagctga acctgcaact cgacgatgaa
      601 tttatccacc acctgatgga tcacgtcggt tgacagatga tcaaaccaga taatgttgtc
      661 gagacggtta cggaattccg gtgtaaagat cttcttgatc tcctccatcg catcggtgct
      721 gttatcctgg tggataagac caatggattt acgctcagtt tcacgtaccc cggcgttggt
      781 ggtcatcacc agcaccacgt tacggaagtc cgctttgcgt ccgttgttat cggtcagcgt
      841 accgttgtcc atcacctgca acagaatatt gaacacgtcc ggatgcgctt tctcgatttc
      901 gtccagcaac agcaccgcat gtggatgctt gatgaccgca tcagtcagca aaccgccctg
      961 atcaaaacca acgtatcccg gaggcgcacc aatcagacgg ctgacggtat ggcgttccat
     1021 atactcggac atatcaaagc gcagcagctc aatgcccaac gctttcgaaa gctgtaccgt
     1081 cacctctgtt ttcccgaccc cggtagggcc ggcaaacagg aacgaaccaa ccggtttatg
     1141 ttcgtgacct aaacctgcac gcgccatctt aatggcttca gtcagcgcct caatggcttt
     1201 atcctgaccg aagaccagca ttttcaggcg atcgccgagg tttttcaggg tatcgcggtc
     1261 actctgagaa acactcttct ctggaatgcg cgcaatacgg gccaccacgg actcaatatc
     1321 cgccacatta acggttttct tgcgtttgct taccggcatc aggcgtgcgc gagcgcccgc
     1381 ttcgtcgata acgtcaatgg ctttatccgg cagatgacga tcgttaatgt atttcaccgc
     1441 cagctctacc gctgcacgca ccgcttttgc ggtataacgc acgtcgtggt gcgcttcata
     1501 cttcggtttc aggccattga tgatttgaac agtttcttcg atcgacggtt cagtaatatc
     1561 aattttctgg aagcgacgcg ccagagcacg gtctttctcg aaaatgttgc tgaactcctg
     1621 ataggttgtc gaaccaatta cgcgaatttt accgctggag agcaacggtt tgatcaggtt
     1681 agccgcatcg acctggccac cagacgctgc acccgcaccg ataatggtgt ggatctcatc
     1741 aataaacagg atgctgttag tgtcctgctc cagctgcttg agcaacgctt taaaacgttt
     1801 ttcaaagtcg ccgcgatatt tagtgcccgc taacagagaa ccgatatcga gagagtaaat
     1861 cgtacagtca gccatcactt ccggcacatc gccctgaaca attcgccagg caagaccttc
     1921 cgcaatcgcg gttttaccga caccagattc ccccaccagc agcgggttgt ttttacggcg
     1981 acggcagaga acctgaatag cacgctccag ctccttctca cgaccaatca gtgggtcgat
     2041 tccgcccacg cgcgcaagct gattcaggtt cgtcgtgaaa ttctccatac gttcctcccc
     2101 accagct
//
LOCUS       DAMZET010000339         2097 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.339, whole genome shotgun sequence.
ACCESSION   DAMZET010000339 DAMZET010000000
VERSION     DAMZET010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.339"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(724..1713)
                     /locus_tag="POB34_004861"
     CDS             complement(724..1713)
                     /locus_tag="POB34_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HDI5921676.1"
                     /translation="MKNPDLQNLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGA
                     RMALCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPR
                     IVADYPDARWMFLTLTVRNCAIGELGEMLNRMNAAFQRMKVRKEFRPVQGWIRTTEVT
                     RGSDGSAHPHFHTLMMVPPGMLNGKSYVRHERWVELWRECLRVDYDPNVDVRAVKPRK
                     PKDGESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGAL
                     KDVLKLDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESG"
BASE COUNT          486 a          645 c          520 g          446 t
ORIGIN      
        1 aaaaaaaaac cgtcttgcag gtggttggcc tcactccgtg acaccaacca ccatccccag
       61 tgtttttccc gtgccgtcgt caatcacaaa atcggaaccc acgccggagg cgcgggcccc
      121 cgcgattttc tgtttgacga atcagggaac ggtgaacggt acgcggaatt ttttcgcgtt
      181 ccgtgaggcg ttggcggggt gtcggggcgc agtcctgacc tggttcgtag aaccgcacag
      241 ggttgcaaag gggtgtcccc tttggcacaa gcgacggagc gaaacgcccc gttttgtgta
      301 cgccgcttat cgtgcctgtg ttttaggaga acaaaaaaat gccgtgcata cagcccacac
      361 aatgacccac ataatcaccc acaactcaaa acacacagcc cacacaatca cccacacttt
      421 aacccacaaa aaagaccaca accccattga caccccacaa aacaacccac acaaacggcg
      481 agtaaatcat tttgccgttt tcgtgccgca gccgttccgc gcaatcgaca aactggcagt
      541 cagggaatct cacgaagtgc gcacttatac aaactcacct taggttcgtt tgtcgtgcgg
      601 tctgcgacgc tcaaaaaacc gagaaacggc gggaattttc gggacaggat gtgtaattgt
      661 aataccgtca cacgcgacgc tatcacaatt gccatctggt caggggtgca ccccgacacc
      721 ccgctagcct gattcagttt tatctttcgt cggtgagcga cggtactttc tgacctcggt
      781 tttccactcg aacgcgatgc gtgaaccgtc atcgtcgccc tcggaaatat catccccgat
      841 caccatgtcg gcgtcggttt cctgatcgag tttcagcacg tctttgagtg cgccgccggt
      901 cgccacaaag cggcgtttgt gcgtctggcg agtcagttcg aggaaccatt caggatcggc
      961 caccatgtca gccggcttcg tggaatattt cagggtttcg gccaccgcac cgcgcaccag
     1021 ttcggcggtc gcgcaggcca gcgattcgcc gtccttcggc ttacgaggtt tcactgcccg
     1081 gacatcaaca ttcgggtcat aatcgacccg cagacactcc cgccagagtt caacccagcg
     1141 ttcatgcctg acgtaacttt tcccatttag catgccaggc ggcaccatca tcagggtgtg
     1201 gaagtgcggg tgtgcgctgc cgtcgctccc gcgggtgact tccgtggtac gtatccagcc
     1261 ctgcacaggc cggaactcct tgcgaacctt catgcgctga aaagctgcat tcatccggtt
     1321 cagcatctcg cccagctccc cgatcgcaca gttgcgcacg gtcagagtca ggaacatcca
     1381 gcgggcatcg ggataatccg ccacgatacg cggcagggac tggtagaaac gggcttgcca
     1441 catcagggag cgcctccact ggcagacagg acaatgccgg acacggcaga aatgcgcctc
     1501 acgcagcctc agacgcgttt ctccggtttc cttgagggta gaccacccga aacgcagtaa
     1561 accaccacag agggccatac gcgccccgta gcgctcatat tcggctgcaa gcaggtaaat
     1621 ccctcccacg tcatcagaaa cgcttctgtg agcgtcccag ggcgcatctg agggggaata
     1681 gtccgtgagg ttctggagat cagggttttt catgcgcacc acccgatagt gggcgatggc
     1741 tcatctgacg tgttatcatt gccatatcac ctgttgtgtt agtcgtatca ccaattcgaa
     1801 ttcctacagg tggtcacacc cccgaacgat tgccctcgtt gcgggggtcg cttttaatgc
     1861 gaccactccc gacagaataa ctccaaaacc attttccgac aaatccccct tcaggccttg
     1921 cggcacaagg gctggaggcg aatttgaatc ctagagttat cccttaagta taaatacaag
     1981 gatcgcgcgc gagattcgct tgaattttga gcgggatttt cagtaaccca aaacacccaa
     2041 ccccacccac ttacacccat ctcatccaca gactttcacc ccgattttgt gaacaac
//
LOCUS       DAMZET010000340         2064 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.340, whole genome shotgun sequence.
ACCESSION   DAMZET010000340 DAMZET010000000
VERSION     DAMZET010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.340"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            195..389
                     /locus_tag="POB34_004862"
     CDS             195..389
                     /locus_tag="POB34_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HDI5921677.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIKSDEE"
     gene            complement(554..760)
                     /locus_tag="POB34_004863"
     CDS             complement(554..760)
                     /locus_tag="POB34_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921678.1"
                     /translation="MNKEQSADDPSVDLIRVKNMLNSTISMSYPDVVIACIEHKVSLE
                     AFRAIEAALVKHDNNMKDYSLVVD"
     gene            1046..1456
                     /locus_tag="POB34_004864"
     CDS             1046..1456
                     /locus_tag="POB34_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HDI5921679.1"
                     /translation="MAQVAIFKEIFDQVRKDLNCELFYSELKRHNVSHYIYYLATDNI
                     HIVLENDNTVLIKGLKKVVNVKFSRNTHLIETSYDRLKSREITFQQYRENLAKAGVFR
                     WVTNIHEHKRYYYTFDNSLLFTESIQNTTQIFPR"
     gene            1747..2040
                     /gene="bor"
                     /locus_tag="POB34_004865"
     CDS             1747..2040
                     /gene="bor"
                     /locus_tag="POB34_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HDI5921680.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKPAAVAPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
BASE COUNT          674 a          450 c          374 g          566 t
ORIGIN      
        1 cctgtcggat caccggaaag gacccgcaaa atgataataa ttatcatctg catgtcacaa
       61 cgtgcatcta cgccatcaaa ccacgtcaaa taatcaatta tgacgcaggt atcgtattaa
      121 ttgatctgca tcaacttaac gtaaaaacaa cttcagacaa tacaaatcag cgacactgaa
      181 tacggggcaa cctcatgtca acgaagaaca gaacccgcag aacaacaacc cgcaacatcc
      241 gctttcctaa ccaaatgatt gaacaaatta acatcgctct tgagcaaaaa gggtccggga
      301 atttctcagc ctgggtcatt gaagcctgcc gtcggagact aacgtcagaa aagagagcat
      361 atacatcaat taaaagtgat gaagaatgaa catcccgcgt tcttccctcc gaacaggacg
      421 atattgtaaa ttcacttaat tacgagggca ttgcagtaat tgagttgcag ttttaccact
      481 ttcctgacag tgacagactg cgtgttggct ctgtcacagg ctaaatagtt tgaatgatta
      541 gcagttatgg tgatcagtca accaccaggg aataatcctt catattatta tcgtgcttca
      601 ccaacgctgc ctcaattgct ctgaatgctt ccagagacac cttatgttct atacatgcaa
      661 ttacaacatc agggtaactc atagaaatgg tgctattaag catatttttt acacgaatca
      721 gatccacgga gggatcatca gcagattgtt ctttattcat tttgtcgctc catgcgcttg
      781 ctcttcatct agcggttaaa atattacttc aaatctttct gtatgaagat ttgagcacgt
      841 tggccttaca tacatctgtc ggttgtattt ccctccagaa tgccagcagg accgcacttt
      901 gttacgcaac caatactatt aagtgaaaac attcctaata tttgacataa atcatcaaca
      961 aaacacaaag aggtcagacc agattgaaac gataaaaacg ataatgcaaa ctacgcgccc
     1021 tcgtatcaca tggaaggttt taccaatggc tcaggttgcc atttttaaag aaatattcga
     1081 tcaagtgcga aaagatttaa actgtgaatt gttttattct gaactaaaac gtcacaacgt
     1141 ctcacattat atttactatc tagccacaga taatattcac atcgtgttag aaaacgataa
     1201 caccgtgtta ataaaaggac ttaaaaaggt tgtaaatgtt aaattctcaa gaaacacgca
     1261 tcttatagaa acgtcctatg ataggttgaa atcaagagaa atcacatttc agcaatacag
     1321 ggaaaatctt gctaaagcag gagttttccg atgggttaca aatatccatg aacataaaag
     1381 atattactat acctttgata attcattact atttactgag agcattcaga acactacaca
     1441 aatctttcca cgctaaatca taacgtccgg tttcttccgt gtcagcaccg gggcgttggc
     1501 ataatgcaat acgtgtacgc gctaaaccct gtgtgcatcg tttttaatta ttcccggaca
     1561 ctcccgcaga gaagttcccc gtcagggctg tggacatagt taatccggga atacaatgac
     1621 gattcatcgc acctgacata cattaataaa tattaacaat atgaaatttc aactcattgt
     1681 ttagggtttg tttaattttc tacacatacg attctgcgaa cttcaaaaag catcgggaat
     1741 aacaccatga aaaaaatgct actcgctact gcgctggccc tgcttattac aggatgtgct
     1801 caacagacgt ttactgttca aaacaaaccg gcagcagtag caccaaagga aaccatcacc
     1861 catcatttct tcgtttctgg aattgggcag aagaaaactg tcgatgcagc caaaatttgt
     1921 ggcggcgcag aaaatgttgt taaaacagaa acccagcaaa cattcgtaaa tggattgctc
     1981 ggttttatta ctttaggcat ttatactccg ctggaagcgc gtgtgtattg ctcacaataa
     2041 ttgcatgagt tgcccatcga tatg
//
LOCUS       DAMZET010000341         2060 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.341, whole genome shotgun sequence.
ACCESSION   DAMZET010000341 DAMZET010000000
VERSION     DAMZET010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.341"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            47..481
                     /locus_tag="POB34_004866"
     CDS             47..481
                     /locus_tag="POB34_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI5921681.1"
                     /translation="MSDVSTNLYKSQLLDYYYQRRAESSINKGSRFLISKAVFGTSSL
                     VTKKGDGTYEIGELPKAFELAELTSQFCTINLVPTYSGGIITVRMDLDQSQLQEGKNY
                     PFNTLVVLDNENKPIAIICVQEDSLYVGKTYTAVMAINTTTA"
     gene            493..>2060
                     /locus_tag="POB34_004867"
     CDS             493..>2060
                     /locus_tag="POB34_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDI5921682.1"
                     /translation="MNDVTVVTSVTYPSSESLALVADVQYHEPYLSAALNRKFRGIVD
                     PGFYAGFLPKPGGGMNLLITSVDGDKTAGAASVDIGEFYQVTIQQRKDISLALSAGKK
                     YAIVLKGRYLLGEDTYQVNTASHIHAAEFVARTYTDSYQLGDGELLVCTVNIPASVSA
                     ITQEMIDTSERINRSIGIDISDSVTSTRSDVAASSLAVKKAYDLAKSKYTAQDASTTQ
                     KGLVQLSSATNSDSETMAATPKAVKSIKDLADTKAPIESPSLTGTPTAPTAAQGTNST
                     QIANTAFVKAAITALINGAPGTLDTLKEIAAAINNDPNFSTTINNALALKAPLASPAL
                     TGIPTAPTAAQGTNNTQIANTAFVKAAITALINGAPGTLDTLKEIAAAINNDPNFSTT
                     INNALALKAPLASPALTGVPTAPTAAQGTNNTQIATTAYVRAAISALVGSSPEALDTL
                     NELAAALGNDPNFATTMTNALAGKQPLDATLTALAALATGANKLPYFTGKDTVAQTDL
                     TSVGRDILAKTSVLA"
BASE COUNT          590 a          506 c          474 g          490 t
ORIGIN      
        1 ttacttatct tacaatgagg cttcacaaca ttgattaggg aaaatcatgt ctgacgtctc
       61 aacaaacctc tataagagtc agttgttgga ctattactat cagcggcgcg ctgaatcgtc
      121 cattaacaaa ggctctcgat ttttaatcag caaggccgtt ttcggtacca gttcgctggt
      181 tactaagaaa ggagatggca cttatgagat tggagaactg ccaaaggctt tcgaactggc
      241 agaactgacc agtcaatttt gcaccatcaa cctcgtccca acctactcag gcgggataat
      301 tactgtccga atggaccttg atcaaagcca gttgcaggaa gggaaaaact acccattcaa
      361 cactctggtt gttctggata acgagaacaa gccaatcgcc attatttgtg tccaggaaga
      421 ctcgctgtat gtgggcaaaa catataccgc agttatggcc ataaacacga ctacagcata
      481 aggatatgct tgatgaatga cgttacagtt gttacatcgg ttacttaccc atcatccgag
      541 tcgttggctc tggtggccga tgtgcaatac cacgaaccat atctgtcagc cgcgctaaac
      601 cgaaaattca gggggattgt tgacccggga ttttatgccg gtttcttacc taagccaggc
      661 ggtgggatga acctgttaat tacctcagtg gatggtgata aaaccgcagg cgcggcgtcg
      721 gtggatattg gtgaatttta ccaggtaact attcagcaac gtaaggatat ttctcttgca
      781 cttagtgcag gcaagaaata tgcaattgtg ctgaagggaa gatacctcct tggagaagat
      841 acctaccagg tgaataccgc gtcacatatt catgcggctg aatttgttgc cagaacctat
      901 accgattcat atcagttagg agatggggag ctgcttgttt gtacggtgaa tatccctgct
      961 agtgtatctg ccattaccca ggagatgatt gatacatccg agcgtatcaa ccgctcgatc
     1021 ggcattgata tttcagactc tgtaaccagt accagaagtg atgttgctgc aagttcgctg
     1081 gcagttaaaa aagcctacga tctggcgaaa agcaagtata cggcacagga tgcaagcaca
     1141 acgcaaaagg gattagttca gctcagtagc gcaactaaca gcgacagcga aacaatggcg
     1201 gctaccccta aagctgttaa gtctataaaa gatctggctg ataccaaagc gccaatagaa
     1261 agcccgagtc tgacaggaac gccaaccgcg ccgacggcag cgcaaggtac aaatagcacg
     1321 cagatcgcaa atacagcctt tgttaaggca gctataacgg cacttatcaa cggtgcacct
     1381 ggcacactgg atacgcttaa agaaatagct gctgcgatca ataacgaccc gaatttcagc
     1441 acaactatca acaatgctct ggctcttaaa gctcctttag caagtcctgc attaacggga
     1501 atacctactg cgcctaccgc tgcacagggt acgaataaca cgcagatcgc aaatacagcc
     1561 tttgttaagg cagctataac tgcacttatc aacggtgcgc ctggcacact ggatacgctt
     1621 aaagaaatag ctgctgcgat caataacgac ccgaatttca gcacaactat caacaatgcc
     1681 ttggctctca aagcgccttt ggcaagccct gcattaacgg gtgtccctac tgcgcctacc
     1741 gctgcacagg gtacgaataa cacgcagatt gctacgaccg cttatgtaag agctgccata
     1801 tccgcattgg ttggttcatc accagaagct cttgataccc tgaatgagct tgccgcagca
     1861 cttggcaatg acccgaactt tgcgacaaca atgacaaatg cgctggcagg caaacagcct
     1921 ctggatgcaa ctttaaccgc tctcgctgcc cttgcgactg gtgcaaacaa actgccttat
     1981 ttcactggta aggatacggt agctcagact gacttaacgt cagtcggtcg cgatattctg
     2041 gccaaaacaa gcgttcttgc
//
LOCUS       DAMZET010000342         2058 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.342, whole genome shotgun sequence.
ACCESSION   DAMZET010000342 DAMZET010000000
VERSION     DAMZET010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.342"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..637)
                     /locus_tag="POB34_004868"
     CDS             complement(<1..637)
                     /locus_tag="POB34_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HDI5921683.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRM"
     gene            complement(716..1396)
                     /gene="narI"
                     /locus_tag="POB34_004869"
     CDS             complement(716..1396)
                     /gene="narI"
                     /locus_tag="POB34_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDI5921684.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            complement(1393..>2058)
                     /gene="narW"
                     /locus_tag="POB34_004870"
     CDS             complement(1393..>2058)
                     /gene="narW"
                     /locus_tag="POB34_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDI5921685.1"
                     /translation="MEYPDELLWECKEDALALIRRDAPMLTDFTHNLLNAPLLDKQAE
                     WCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLVEYEKVGLQLDCRELPDYLPLYLE
                     YLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGSSLSSDSVTKQ
                     VNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDVAPQYVGISAG
                     GGK"
BASE COUNT          508 a          587 c          521 g          442 t
ORIGIN      
        1 acatgcgacc atcggtttcg tttttgcctg ggcggatctg gaacgggaag aaaccattgc
       61 agccaatctg tttactgata gcggtcagcg cattaaggtc aggcgaaaga gcgtcgatat
      121 ccacttccgg cttcaatggg atcatcactt tagaatgccc ggtcgtcgcg acctggatcg
      181 gcaaacccgg cagaatatcg tcttcagtaa gatgcaatgc gttgataatc gccgcgcgta
      241 cttcaccctc cagcggcagt tcaaagcccg gcgtaccttg ttccagcgaa atacgataat
      301 catcgttgtg cttttcgata gtcacgcgat gttttcctgc cagcgatgtt tgccagatcg
      361 tgcaatttcc taaacctaac accttcgcac gtacatagtg cgcagctact gtcgcgtgac
      421 cgcaaatcgg cacttcaacc gttggcgtaa agtagcggat gcgcacatcg ctgtcgtcgc
      481 tgtgcagcag aaaagcagtt tccgaatggc ctaactcgcg ggcgataagc tgcatttgcg
      541 cttcgctaag attatcggcg gggaaaacaa caccggcaga attgccgcga aacggttgtg
      601 aggtaaaagc atcgacgtgg tacacctgcg gtttcattaa cgttctcctg tgactggaga
      661 actatcatag cctgcaagtg gccggagagc gaagggctat ccggccaggg tgaaattatc
      721 gccgcgaacg cacaatttga tatcgacgag taaagtactc aaacggcgcg ctccacacat
      781 gcaccagtcg ggtgaacggg aagagcagga agatggtcat tcccaacacc agatgcaggc
      841 ggaagacgaa cgctacgccg ttgagcatct ctgacgatcc accacggaaa gtgacaatgc
      901 tttgcgccca acccaccagc ttcatcattt cgctaccatc aggatactgt gctgaaaacg
      961 gtatggtact tagccccaac agacactgga tcagcaaaat gctcatgatg atgatatccg
     1021 gcgtagtgga agtggcacgc acgcgctgat ttgtcagcct gcgccacagc agtcctgcgc
     1081 cgccaatcaa cgtcaacaca ccgcaaatac cgccgagcac catcgccatg agttgtttcg
     1141 ctgccactgg taaaaaccac gcgtacatcc agtgcggcgt taacatgccg aacaagtgcc
     1201 cgaagaaaat ccccaaaatg ccgatatgga acagattcga ccatatcacc atcccgcgtt
     1261 tatcgagcat ttgacttgag gaggcgcgcc aggtgtactg cccgtagtcg tagcgcagcc
     1321 agctgccgag gaaaaacacc gtcgcacaaa tgtacgggta gatatcgtaa aaaaagacgt
     1381 tcagatactg aatcatttcc cacctcccgc actgatgccg acatattgcg gcgcgacatc
     1441 ctggctaaag cgtcgctgat attgattaag cggcgagctg tcacacgccg tggcgttatc
     1501 ttcaataaac ttcacctgtt cctcttccca cactgcatca agcgcttggc gggtgtcatc
     1561 gcgctcttcg ctgtttactt gttttgtgac actgtcactt gaaagggagc ttcccgccag
     1621 ttgcagcaga gcatcaaaca acgcgtacca cggtgcctcg cgttgtttta agcgaccgcc
     1681 aagcagggcg aggatcggcg caacattgag caatccttct ttcgcctgat cgtcaggcag
     1741 tacgcttaaa tactccagat acagcggtag ataatcgggc agttcccgac aatccagctg
     1801 caggccgacc ttttcatact ccaccagcag gtccaccatt gcctggccgc gatcgcggga
     1861 ctcggcatga acatgttcga acagcagcag cgacgtggtg cgcccgcggt caaacacttc
     1921 gcaccattcg gcctgtttat ccagcagcgg cgcgttaagc aggttgtggg tgaaatccgt
     1981 aagcatcggc gcgtcgcggc ggatcaacgc cagcgcgtcc tccttgcatt cccacaacaa
     2041 ctcatccgga tactccat
//
LOCUS       DAMZET010000343         2052 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.343, whole genome shotgun sequence.
ACCESSION   DAMZET010000343 DAMZET010000000
VERSION     DAMZET010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.343"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..761
                     /gene="ldtE"
                     /locus_tag="POB34_004871"
     CDS             <1..761
                     /gene="ldtE"
                     /locus_tag="POB34_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HDI5921686.1"
                     /translation="TIPSQLLLPDAPRQGIIVNLAELRLYYYPPGENIVQVYPIGIGL
                     QGLETPVMETRVGQKIPNPTWTPTAGIRQRSLERGIKLPPVVPAGPNNPLGRYALRLA
                     HGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIKALFSSVRTGTPVKVINEPVKYSVE
                     PNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQFKDNKAVDQKLVDKALYRRAGYPV
                     SVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ"
     gene            complement(825..1061)
                     /gene="lpp"
                     /locus_tag="POB34_004872"
     CDS             complement(825..1061)
                     /gene="lpp"
                     /locus_tag="POB34_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HDI5921687.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(1372..>2052)
                     /locus_tag="POB34_004873"
     CDS             complement(1372..>2052)
                     /locus_tag="POB34_004873"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDI5921688.1"
                     /translation="GDLGVEIPVEEVIFAQKMMIEKCIRARKVVITATQMLDSMIKNP
                     RPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICERTDRVMNSRLEFN
                     NDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFPDATILALTTNEK
                     TAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVVMVSGALVPSGTT
                     NTASVHVL"
BASE COUNT          529 a          480 c          516 g          527 t
ORIGIN      
        1 taactattcc ttcacaactg ttattacctg atgcaccgcg tcaggggatt atcgttaacc
       61 ttgcagagct gcgcctttat tattatccgc cgggagaaaa tattgtgcag gtttatccaa
      121 taggtattgg attgcagggg ctggaaacgc cggtgatgga aacgcgtgtt gggcagaaaa
      181 tccctaaccc aacttggacg cctacggcag gcattcgtca acgttcgctg gagcgtggca
      241 ttaaattacc gccagtcgtt cctgccggac caaataaccc gctaggacgt tacgcactgc
      301 gcctcgcgca tggtaatggc gaatacctca ttcatggtac cagtgcgccg gacagcgtcg
      361 gtttgcgcgt cagttcaggg tgtattcgca tgaatgcacc ggatattaaa gccttgttct
      421 ccagcgtgcg gacgggaacg ccggtgaaag tgatcaacga accggtgaaa tattccgtgg
      481 aacctaacgg gatgcgttat gttgaagtac atcgaccact atcagcagaa gaacagcaaa
      541 acgttcagac aatgccatac acactgccag caggctttac gcaatttaaa gacaataagg
      601 ctgtagatca gaagttagtc gataaagcgt tgtatcgtcg ggcagggtat ccggtttcgg
      661 tgagcagtgg agcaactccc gcagccagca atgcgccttc agtagagtca gcgcagaatg
      721 gtgaaccaga gcaagggaat atgttacgcg cgacgcagta gcggtaaacg gcagacaaaa
      781 aaaatggcgc acaatgtgcg ccatttttca cttcacaggt actattactt gcggtattta
      841 gtagccatgt tgtccagacg ctggttagca cgagctgcgt catctttagc agcctgaacg
      901 tcggaacgca ttgcgttcac gtcgttgctc agctggtcaa ctttagcgtt cagagtctga
      961 acgtcagaag acagctgatc gattttagcg ttgctggagc aacctgccag cagagtagaa
     1021 cccaggatta ccgcgcccag taccagttta gtagctttca ttattaatac cctctagatt
     1081 gagttaatct ccatgtagcg ttacaagtat tacacaaagt tttttatgtt gagaatattt
     1141 ttttgatggg aaggcactta tttttgatcg ttcgctcaaa gaagcatcga acgctgggtt
     1201 ttagataaaa aaattgagag aaaacaggat gcttccatcg gattcatctt agataaagtg
     1261 agattatata gtgaaatccg atttgatttt ttattgcttc tggttatcga ttaaataaaa
     1321 aaagcgccca tcagggcgct tcgatataca aattaattca caaaagcaat attacaggac
     1381 gtgaacagat gcggtgttag tagtgccgct cggtaccagt gcaccagaaa ccataactac
     1441 gacgtcacct ttgtgtgcca gaccgctctg cagagccagt tctttaccca gacggtagaa
     1501 atcatcagta gaagtgatct ctttaaccag ctgcggcaca acgcctttgc tcagtaccaa
     1561 ctgatgagcc gttttttcgt tggtggtcag tgccaggatg gtggcatccg ggaagtattt
     1621 acgtactgcg cgagcagatt taccgccctg ggtagcaacc acgatcagcg gagcatccag
     1681 tttttcagca gtttcaacgg caccacggca taccgcttcg gtaatgcgca gtttacggtt
     1741 gtcattgttg aactcgagac ggctgttcat cacgcggtcg gtacgttcgc agatggtcgc
     1801 catgatagaa accgcttcca gcgggtattt accttttgcg gattcaccag acagcatcac
     1861 tgcgtcagta ccgtcgagga tggcgtttgc aacgtcaccg gcttctgcgc gagtcgggcg
     1921 tgggtttttg atcatggaat ccagcatctg ggtcgcagtg ataacgactt tacgtgcacg
     1981 gatacatttt tcgatcatca tcttctgggc gaagataact tcttctaccg ggatttctac
     2041 acccaggtcg cc
//
LOCUS       DAMZET010000344         2018 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.344, whole genome shotgun sequence.
ACCESSION   DAMZET010000344 DAMZET010000000
VERSION     DAMZET010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.344"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..480
                     /locus_tag="POB34_004874"
     CDS             <1..480
                     /locus_tag="POB34_004874"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDI5921689.1"
                     /translation="QQWVSQAGAQDCVLALENESNPHNLGGMMRSCAHFGVKGVVVQD
                     AALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTSSEQGKPLFKTSL
                     PAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGVLLGEWWRQNKA"
     gene            complement(528..1217)
                     /gene="ung"
                     /locus_tag="POB34_004875"
     CDS             complement(528..1217)
                     /gene="ung"
                     /locus_tag="POB34_004875"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDI5921690.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            1522..1905
                     /gene="grcA"
                     /locus_tag="POB34_004876"
     CDS             1522..1905
                     /gene="grcA"
                     /locus_tag="POB34_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HDI5921691.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
BASE COUNT          538 a          483 c          523 g          474 t
ORIGIN      
        1 cagcagtggg taagccaggc aggcgcgcag gattgtgtac tggcgctgga aaacgaatct
       61 aacccgcata acctgggtgg catgatgcgc agctgcgcgc acttcggcgt gaaaggcgtt
      121 gtggtgcagg atgcggcact gctggagtcg ggggcggcta tccgtaccgc agaaggcggc
      181 gcagagcacg ttcagccgat tactggcgac aacattgtta acgtgttgga tgatttccgt
      241 caggcaggtt acaccgtagt gaccacttcc agcgagcagg ggaaaccgct gttcaaaacc
      301 agtctgccag cgaaaatggt actggtgctg ggtcaggaat atgaagggtt accggatgcc
      361 gcacgcgatc cgaacgatct gcgcgtgaag attgacggta cgggcaacgt tgccgggctg
      421 aatatctctg ttgcaaccgg cgttttgctt ggtgaatggt ggcgtcagaa taaagcctga
      481 taaatcagcc gggtggcaac tctgccatcc ggcattttac cgcaaattta ctcactctct
      541 gccggtaata ctggcatcca gtcaatcggc gtctcgccac gttgttccag ccactgattt
      601 gccagcacaa aatggttgca gccaaagaat ccacgatgcg ccgaaagcgg cgacggatgc
      661 ggtgctttca gtacatgatg gcgttgctta tctataatcg cccctttctt ttgcgcatgt
      721 gatccccaca acaaaaacac cacgccttcg cggtgctggt taatcaggct gatgacttta
      781 tcagtaaacg tctcccaacc gaggctggcg tgggaatgcg cctgacctgc gcgaaccgtc
      841 aacacagtat tgagcagcag aacgccctga cgcgcccagc tttcgagata gccatgatta
      901 gggcgggtga agcccggaat agtattttcc agctctttat acatattcaa taatgacgga
      961 ggaatggcaa tgccgggacg aacggaaaat gccagaccat gcgcctgtcc cggtccgtga
     1021 taaggatcct ggccgagaat caccacttta acgtcaccca actcggtaaa gcggaacgcg
     1081 ttaaagacat ctttttgtgg tggatagata gtgacgccgg actgccgctc gctggcgacg
     1141 gtctgaaggg tattaagaaa ataaggttgt tgcttctctt cagccagcac gtcatgccag
     1201 gttaattcgt tagccatctc gctctcctgc gaatcttcaa tccgcctagc ttaactgctt
     1261 ctttctacag aacaaaatca tgctcatttt ttagacttaa acgagtgcaa aaaaatgtaa
     1321 attggtaaaa tttcacaccc gcataagttg atgtaaaaca ataaaatcca tttacgacca
     1381 caatcgatgt gtggttttta ttgatttaaa tcaaagaatc aagggtggtt gaggagtata
     1441 tatacactca agcaacaatg gttttaccaa ttggccgcga caggctgaac aaatcaaata
     1501 attttgccgg ggaggcatca catgattaca ggtatccaga ttactaaagc cgctaacgac
     1561 gatctgctga actctttctg gctgctggac agcgaaaaag gcgaagcacg ttgcatcgtt
     1621 gcaaaagcag gttatgcaga agatgaagtg gttgcagtaa gcaaactggg tgacattgaa
     1681 taccgtgaag ttccagtaga agtgaaacca gaagttcgcg ttgaaggtgg tcaacacctg
     1741 aacgttaacg ttctgcgtcg tgaaactctg gaagatgcag ttaagcatcc ggaaaaatat
     1801 ccgcagctga ccatccgtgt atccggttat gcagttcgct ttaactctct gactccggaa
     1861 cagcagcgcg acgttatcgc tcgtaccttt actgaaagcc tgtaaggctt aagtagcaag
     1921 ttactaagcg gaagagggcg acctcttccg ctttttttcg ttaatagaaa atgcgtaccg
     1981 cgcaatagac cagcaacagg gcaagcacga tatttaac
//
LOCUS       DAMZET010000345         2002 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.345, whole genome shotgun sequence.
ACCESSION   DAMZET010000345 DAMZET010000000
VERSION     DAMZET010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.345"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..82
                     /locus_tag="POB34_004877"
     CDS             <1..82
                     /locus_tag="POB34_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HDI5921692.1"
                     /translation="GPVDFNVFHGAVEELQAFVDGIKETP"
     gene            134..880
                     /locus_tag="POB34_004878"
     CDS             134..880
                     /locus_tag="POB34_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI5921693.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(854..1819)
                     /locus_tag="POB34_004879"
     CDS             complement(854..1819)
                     /locus_tag="POB34_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDI5921694.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(1816..>2002)
                     /locus_tag="POB34_004880"
     CDS             complement(1816..>2002)
                     /locus_tag="POB34_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDI5921695.1"
                     /translation="CANLGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDF
                     NRYATALAKYRQQREAV"
BASE COUNT          478 a          549 c          538 g          437 t
ORIGIN      
        1 tggtccggtg gattttaatg tatttcatgg cgcggtggag gagttgcagg cattcgttga
       61 tgggattaaa gaaacgcctt aaataagaag cagtgatata ctcccaccag actcctcggc
      121 aggaaaaaga caaatggaac aagcgcatac ccagctgatt gcccaactga acgagcgtat
      181 tttagcggcg gataacacgc cgctttatat taagtttgcc gaaacggtaa aaaatgccgt
      241 gcgcagcggg gtgctggagc atggcaatat tttgcccggt gagcgtgatt taagtcagtt
      301 aaccggcgtt tcgcgcatta cggtgcgcaa ggcgatgcag gcgctggaag aagagggtgt
      361 ggtgacgcgt tcgcgcggtt acggcacgca gatcaacaac atcttcgaat actcgttgaa
      421 agaagcgcga ggtttttctc agcaggtggt attgcgcggg aaaaagcccg atacgctatg
      481 ggttaacaag cgtgtcgtga aatgtcccga agaagtcgcg cagcagctgg cggtcgaagc
      541 aggaagtgat gtctttttgc ttaagcgtat tcgctatgtc gatgaagaag cggtatcgat
      601 tgaggaatcg tgggttccgg cgcatttaat tcatgatgtt gatgccatcg ggatttcact
      661 ttatgactat ttccgcagcc agcacattta cccacaacgt acgcgttccc gcgttagcgc
      721 ccggatgccg gatgccgagt ttcagtcaca tattcagtta gatagcaaaa taccggtgct
      781 ggtgatcaag caagtggcgc ttgaccaaca gcaacggcct attgagtaca gcattagcca
      841 ctgtcgcagc gatctatacg tttttgtgtg cgaggagtag ttcctcgcgc gttggcgcac
      901 aatcaccgcc ccgatgcccg acaacccacg acgccactgc attgcccagt aatacggcat
      961 ccgccagtgg cagaccagag gccagcccgg caagtacgcc acctgcatga ctgtcgcccg
     1021 ccccaatggt gtctacaact tgcgttggaa atgccggaat gcagccagaa gcgtcgttgc
     1081 tgaaatacca tgcaccctct ttatcgagac gaacgatcaa cggcgcggca aatttctcct
     1141 gccattgctt gccaagtgtt gttatctctg cggataaagc aaaacgttcg gcggcaatct
     1201 cagcctcttg acgattgagt gatactaaag gtcgacaggc catgatccgc gccagtaatg
     1261 cgtccgggat atcgccgata cgtgggccaa aatcgataaa cggcgtcacg tcttgcagct
     1321 tttccagcca ttccactaac aattcgccgc agggcgaggc cagttgataa ccggaaaaat
     1381 agagcaggct gccaggcgca acggttaatc gcgccagcca ctggcgattc cactgatttt
     1441 ccacaccgct gaatgacata aaagtgcgtt cgccatccgg ctcaaccagc gccagacacc
     1501 agccgttatc gccttcggcg ttatcgatca ggctgattaa cccctctttc gccatccggt
     1561 tgcgaataat ctccgcccac acgccctgac cgagcggcaa ggcattaccc gcttcgatgc
     1621 cgaggcgctt taacgccacg gcaatattca gtgcgcagcc gccaacgtta acgctctgct
     1681 gcttcagttc gatatcgcac ccacgccagg ggagggcata agcgtcggcg atcacatcaa
     1741 tgaccgccgc gccgacgacc atcaccggct gacgcgaggt taattcaggc agcagcgtgt
     1801 gtaatcgagc gccgctcata ccgcctcccg ttgttgacga tatttcgcca gcgctgtggc
     1861 atagcggttg aaatcaagct gatttaccgc atccagttcc gcctttaatg caggatcgat
     1921 agcgttaacg ccatgcaacg cgccgcaaat cgccgtcgcc atagcgccga tggtgtctgt
     1981 gtcgccgcca aggttagcgc ac
//
LOCUS       DAMZET010000346         1921 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.347, whole genome shotgun sequence.
ACCESSION   DAMZET010000346 DAMZET010000000
VERSION     DAMZET010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.347"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..416)
                     /locus_tag="POB34_004881"
     CDS             complement(<1..416)
                     /locus_tag="POB34_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HDI5921696.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFARTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLG"
     gene            complement(427..1146)
                     /locus_tag="POB34_004882"
     CDS             complement(427..1146)
                     /locus_tag="POB34_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDI5921697.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPMYLADEPEWASRAA
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELFTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(1674..>1921)
                     /gene="rclR"
                     /locus_tag="POB34_004883"
     CDS             complement(1674..>1921)
                     /gene="rclR"
                     /locus_tag="POB34_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HDI5921698.1"
                     /translation="HMSRASFAQLFRDVSGTTPLAVLTKLRLQIAAQMFSREMLPVVV
                     IAESVGYASESSFHKAFVREFGCTPGEYRERVRQLAP"
BASE COUNT          502 a          499 c          389 g          531 t
ORIGIN      
        1 cccagatcgg tttcaatcac ctgaatgcca gcatcctgca acacatgatt gacaccaatc
       61 tcttcggtca ccatcgattt agatttcacc accttccggg catttttgcg ttgggcaacc
      121 tgtaaaatgt agcgggtagc gtcttctttg gttcttgcaa aatagacgtg accgccgttt
      181 tgcgtcactt tttctgagag ctggtacaga taagcgtcga gattactcag aacatgatca
      241 cgtatctggg cggcccgatc gcgccactcc tcccagtgcc ccaattcatc gaccattttt
      301 tgccgatttg ccccaatacg ctgctgcgcg tttgccaccg ctttgcgcat gatcggatct
      361 tcaatttgct gacggatgcg tgtcttaaaa tctgtattac tggttttgat cgacatcttt
      421 atatcctcag cggctcatca acacttcagc aatatgcatc actttgactt tctgcccttc
      481 ccgttgtaat cgcccactga tgtttagcag gcaactcacg tcagcaccaa ttaaatactc
      541 agggcggact tccatcaggt gcgcaacctt ttctttcacc atctcgccgg atatttcggc
      601 cattttgacc gagaacgtgc cgccaaatcc gcagcaggta tcctgttcag caaaggtaaa
      661 cagctccagt ccacgcacat ttttcagcag cgtaagtggc tcgtccttca ctcccagctt
      721 acgggccagg ctacaagatg ggtgatacac cgctctccct tgcaaactgg cacctacatc
      781 gactacccct aatttattaa caataaaaga ggtgagatcc tgcatacgcg cggcaacctt
      841 tgcggcacgt gatgcccatt caggttcatc cgccagatac atcgggtaac tttttacggc
      901 ataggtgcag gagccagccg gtgaaataat gggatcatcg ttatcctcca gcgcggcgat
      961 cagatttttc atccctggaa tcgcttcttt gatataaccg ctattgatcg caggctgacc
     1021 gcagcatccc tgtttctccg ggaaatttac gcgacagccg agtttttcca gtagcagcac
     1081 ggagtctcgt gccattcttg atttcagggc gtcaccaata caggtgacaa agaaattgac
     1141 attcaaaact aatactccat tacttcatgc ccatttatgc ccgatgtttc ccccccaccg
     1201 ggggatcaat catcaataaa tatggcgttc ttttttaatt ggctagcaac ataccacccc
     1261 tcatttgtat gacaaatgat aatctttcat ccaaacgtca aatgcaataa ctaattttat
     1321 ttctttcggc atcgctcaca ttttattggg aaatcgacga tcatttttgg cataagaaat
     1381 ccattttaaa atggctacat cccttatacc tattcagagc tacttattca ggggttattg
     1441 agatatacac tgatggtgta caaaaaaatc accgacaata atcacgtatc aagtaatagt
     1501 cgggatttta tttaattgaa gcaatttagt ttgcattttt atttaaaaac tgagctagag
     1561 tccatcagaa aaaaatatat aaagcccaaa ccaccgttgg aaatagaaat agttgaaagg
     1621 ctcacaactc ccggaatgcg gattatcttt gccctgattt ctggcggttt tattcagggt
     1681 gcaagctgtc tgaccctttc ccgatattct cccggagtac aaccaaactc gcggacaaac
     1741 gccttgtgaa aagatgattc actggcatag cctactgact cagcgatcac cacaacaggg
     1801 agcatttccc gggaaaacat ctgggccgct atttgtagac gcaactttgt taatacagcc
     1861 agcggcgtgg ttccggaaac atcacggaaa agctgggcaa aacttgcccg ggacatgtgg
     1921 g
//
LOCUS       DAMZET010000347         1905 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.348, whole genome shotgun sequence.
ACCESSION   DAMZET010000347 DAMZET010000000
VERSION     DAMZET010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.348"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>1905
                     /locus_tag="POB34_004884"
     rRNA            <1..>1905
                     /locus_tag="POB34_004884"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          487 a          425 c          603 g          390 t
ORIGIN      
        1 tgtttcggca agggggtcat cccgacttac caacccgatg caaactgcga ataccggaga
       61 atgttatcac gggagacaca cggcgggtgc taacgtccgt cgtgaagagg gaaacaaccc
      121 agaccgccag ctaaggtccc aaagtcatgg ttaagtggga aacgatgtgg gaaggcccag
      181 acagccagga tgttggctta gaagcagcca tcatttaaag aaagcgtaat agctcactgg
      241 tcgagtcggc ctgcgcggaa gatgtaacgg ggctaaacca tgcaccgaag ctgcggcagc
      301 gacgcttatg cgttgttggg taggggagcg ttctgtaagc ctgtgaaggt gtgctgtgag
      361 gcatgctgga ggtatcagaa gtgcgaatgc tgacataagt aacgataaag cgggtgaaaa
      421 gcccgctcgc cggaagacca agggttcctg tccaacgtta atcggggcag ggtgagtcga
      481 cccctaaggc gaggccgaaa ggcgtagtcg atgggaaaca ggttaatatt cctgtacttg
      541 gtgttactgc gaagggggga cggagaaggc tatgttggcc gggcgacggt tgtcccggtt
      601 taagcgtgta ggctggtttt ccaggcaaat ccggaaaatc aaggctgagg cgtgatgacg
      661 aggcactacg gtgctgaagc aacaaatgcc ctgcttccag gaaaagcctc taagcatcag
      721 gtaacatcaa atcgtacccc aaaccgacac aggtggtcag gtagagaata ccaaggcgct
      781 tgagagaact cgggtgaagg aactaggcaa aatggtgccg taacttcggg agaaggcacg
      841 ctgatatgta ggtgaagtcc ctcgcggatg gagctgaaat cagtcgaaga taccagctgg
      901 ctgcaactgt ttattaaaaa cacagcactg tgcaaacacg aaagtggacg tatacggtgt
      961 gacgcctgcc cggtgccgga aggttaattg atggggtcag cgcaagcgaa gctcttgatc
     1021 gaagccccgg taaacggcgg ccgtaactat aacggtccta aggtagcgaa attccttgtc
     1081 gggtaagttc cgacctgcac gaatggcgta atgatggcca ggctgtctcc acccgagact
     1141 cagtgaaatt gaactcgctg tgaagatgca gtgtacccgc ggcaagacgg aaagaccccg
     1201 tgaaccttta ctatagcttg acactgaaca ttgagccttg atgtgtagga taggtgggag
     1261 gcttagaagt gtggacgcca gtctgcatgg agccgacctt gaaataccac cctttaatgt
     1321 ttgatgttct aacgttgacc cgtaatccgg gttgcggaca gtgtctggtg ggtagtttga
     1381 ctggggcggt ctcctcctaa agagtaacgg aggagcacga aggttggcta atcctggtcg
     1441 gacatcagga ggttagtgca atggcataag ccagcttgac tgcgagcgtg acggcgcgag
     1501 caggtgcgaa agcaggtcat agtgatccgg tggttctgaa tggaagggcc atcgctcaac
     1561 ggataaaagg tactccgggg ataacaggct gataccgccc aagagttcat atcgacggcg
     1621 gtgtttggca cctcgatgtc ggctcatcac atcctggggc tgaagtaggt cccaagggta
     1681 tggctgttcg ccatttaaag tggtacgcga gctgggttta gaacgtcgtg agacagttcg
     1741 gtccctatct gccgtgggcg ctggagaact gaggggggct gctcctagta cgagaggacc
     1801 ggagtggacg catcactggt gttcgggttg tcatgccaat ggcactgccc ggtagctaaa
     1861 tgcggaagag ataagtgctg aaagcatcta agcacgaaac ttgcc
//
LOCUS       DAMZET010000348         1882 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.349, whole genome shotgun sequence.
ACCESSION   DAMZET010000348 DAMZET010000000
VERSION     DAMZET010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.349"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..377
                     /locus_tag="POB34_004885"
     CDS             <1..377
                     /locus_tag="POB34_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HDI5921699.1"
                     /translation="LGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQV
                     HYWWVGLGNVIGSTILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALM
                     LIIGWEKRFFRRAAPQTAKEIA"
     gene            374..607
                     /gene="yedF"
                     /locus_tag="POB34_004886"
     CDS             374..607
                     /gene="yedF"
                     /locus_tag="POB34_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDI5921700.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            716..1387
                     /locus_tag="POB34_004887"
     CDS             716..1387
                     /locus_tag="POB34_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HDI5921701.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQDIGGKEASEIAASGCVPANQFSWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(1372..>1882)
                     /locus_tag="POB34_004888"
     CDS             complement(1372..>1882)
                     /locus_tag="POB34_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI5921702.1"
                     /translation="SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHA
                     MIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGG
                     QVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVL
                     ACGGCQQTG"
BASE COUNT          413 a          479 c          507 g          483 t
ORIGIN      
        1 agttaggcgt tgaacccaaa atcatgtggg cgggaccaaa cgcggtaatt ggtggtttac
       61 tgtttggttt tggcatcgtg ctggctggcg gctgcgaaac cggctggatg taccgcgcgg
      121 tagaaggcca ggtgcactac tggtgggtcg gtctgggcaa tgtgatcggc tcaacgattc
      181 tggcgtatta ctgggatgat ttcgctccgg cgctggccac cgactgggac aaaatcaacc
      241 tgctgaaaac ctttggtccg atgggtggcc tgctggtgac atatttgctg ttgtttgctg
      301 cgctgatgtt gattatcggc tgggaaaaac gcttcttccg ccgtgcggca ccgcagactg
      361 ctaaggagat cgcatgaaaa atatcgttcc tgattaccgc cttgatatgg tgggtgaacc
      421 ctgcccttat ccagcagtcg caacccttga ggcgatgccg cagttaaaaa aaggggaaat
      481 cctggaagtg gtgagcgact gtccgcagtc gatcaataat attccactgg atgcgcgtaa
      541 tcacggctat acggtgctgg atattcagca agacgggccg accattcgtt atttaattca
      601 gaagtaatcc ctcattcccg ctgtgaccca cagcgggaac atcgttacct ttgtatcaca
      661 tagattttac ccaccccctc tcctattctt ttcagtatga tctcaggagg tttctatgtg
      721 tggacgcttt gcccaatccc aaacgcgtga agattacctt gcgcttctcg cggaagatat
      781 tgaacgcgat attccctacg atcccgaacc cattggcaga tacaacgtcg cgccgggaac
      841 caaagttctt ctgctcagtg aacgtgatga acaccttcat ctggatccgg ttttctgggg
      901 atatgctccc ggatggtggg ataaaccgcc gctgattaac gcccgcgtag aaactgcggc
      961 caccagtcgt atgtttaaac cgctctggca acatggtcgg gcaatctgtt ttgccgatgg
     1021 ctggtttgag tggaaaaaag aaggcgacaa aaaacagcct tattttatct atcgcgctga
     1081 tgggcaacct atttttatgg ccgcgatagg tagcacacca tttgagcgtg gtgacgaagc
     1141 cgaaggattt ttaattgtca ctgctgcagc agaccagggt ctggtagaca ttcatgaccg
     1201 ccgcccactg gtactgtcgc cagaagctgc gcgggaatgg atgcggcaag acattggcgg
     1261 taaagaagcc tcagaaatcg cggccagtgg ctgtgtgcca gcaaaccagt tctcctggca
     1321 cccggtatcg cgcgcggtag gtaatgtcaa aaaccagggc gcggagttaa ttcaacctgt
     1381 ttgttgacat cctccacagg caagaacggc gtgccccgcc gctaaaatgt tacgagcgcc
     1441 gtttacatcg gcgttcgctg tatatccaca tacctggcat ctgaatttac tttgtgacag
     1501 gcggttttct ttcgctgtat gaccacagca cgcgcaacgc tggcttgtgt acgctggcgg
     1561 aacagcaagc acctgaccgc cacgccagag ctgcttgtac gcaagctggc ggcgcatttc
     1621 ataccagccc tgatccagta tcgaacggtt taaacctgat tttgcccgga cattgcgccc
     1681 cggctggctg accgtacccg ctgctgactt tgacatgttg ctgaccttca aatcctcaat
     1741 gactatcatt gcgtggtttt tgctgacggt cgttgtgact ttgtgaaggt agtccctgcg
     1801 gatatttgcg atacaggaat gcagtcgctg tattttgcgt ttctgcttct gccagttgtt
     1861 gctgaatttg accttgcggc tt
//
LOCUS       DAMZET010000349         1863 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.350, whole genome shotgun sequence.
ACCESSION   DAMZET010000349 DAMZET010000000
VERSION     DAMZET010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.350"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..995
                     /locus_tag="POB34_004889"
     CDS             <1..995
                     /locus_tag="POB34_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI5921703.1"
                     /translation="SLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCRQ
                     LLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFPG
                     CHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLFA
                     GQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFPD
                     SEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVSA
                     AGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            1220..>1863
                     /gene="opgD"
                     /locus_tag="POB34_004890"
                     /gene_synonym="mdoD"
     CDS             1220..>1863
                     /gene="opgD"
                     /locus_tag="POB34_004890"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HDI5921704.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFT"
BASE COUNT          453 a          471 c          478 g          461 t
ORIGIN      
        1 cttctctggc gcgtaatccc ttccgcgttt ttacctcctt actccgcctt gagcttatcg
       61 agaacgaaat tttgcgccag aaagcggcgg agattctgcg tcagcgcgat atcttcaccc
      121 cacgttgtcg acaactgtta gaggaatatg agcagcaggg cggttttaac gaaacacagg
      181 cacaggagtt tgtgcaggaa gccctggaaa cgtttcgctg gcaccagtca gcaacggtag
      241 atgaagaaac ctatcgcgcc ttgcacaacg aacatcggtt gattgctgat gtggtctgtt
      301 ttcctggatg ccatatcaac cacctgacac cacgtacgct ggatattgac cgggtgcagt
      361 cgatgatgcc tgaatgcgga attgaaccca aaattctcat cgaggggccg ccgcgccgcg
      421 aggtaccgat tttactacgc cagaccagct ttaaagcact ggaagagacg gtgttgtttg
      481 cggggcagaa acagggcacg cataccgcgc gctttggtga aattgagcag cgtggcgtgg
      541 cattaacgcc gaaagggcga caactgtatg atgatcttct gcgtaacgct ggaaccgggc
      601 aggataatct cactcaccaa atgcatttac aggaaacctt ccgcactttt cctgacagtg
      661 agtttttaat gcgtcagcaa gggctggcat ggttccggta ccgtctgacg ccttcaggtg
      721 aggcgcatcg tcaggcgatt catcccggag acgatccaca gcccttaatt gaacgtggtt
      781 gggtcgcggc gcaacccatt acctatgaag atttcttgcc cgttagcgcg gcggggatct
      841 tccagtcaaa tctgggtaat gaaacgcaga cacgcagtca cggtaatgcc agtcgcgaag
      901 catttgagca ggcgttgggt tgtccggttt tggatgagtt ccagctttac caggaagcgg
      961 aagaacgcag taaacgtcgc tgtggtttgc tttaaattct gaccatccgc ctttgcaaaa
     1021 atttgcctga ttttacaaac gaatcaggct catcccatcg acataaaaaa aatgccgatt
     1081 tatgcatatt ctctcagttc aacaattgga ttattaataa atattgtcta gagtgagcgg
     1141 tcataaataa gcactttctt gccgctgaaa acgaccagcg cgggaccatt cacaacacca
     1201 gaaggactca ctttcaggta tggatcgtag acgatttatt aaaggttcaa tggctatggc
     1261 cgccgtgtgc ggtaccagcg gcattgcttc tcttttttct caggcggcat tcgcggcaga
     1321 ttctgatatt gccgacgggc aaacccagcg ttttgacttc tccattctac agtcaatggc
     1381 gcacgactta gcgcaaacag cgtggcgtgg tgcgcctcgt ccgttacctg acacgctggc
     1441 gacaatgacg ccgcaggctt ataacagtat tcaatacgac gccgaaaaat cgctctggca
     1501 taacgttgag aaccgtcaac tggacgctca gttcttccat atgggaatgg gattccgtcg
     1561 ccgcgttcgt atgttttctg tagatccagc aacacatctg gcgcgtgaaa ttcactttcg
     1621 cccggagttg ttcaaataca acgatgcggg tgttgataca aaacaattag aagggcaaag
     1681 cgatctcggc tttgccggtt ttcgcgtgtt taaagccccc gaactggcgc gccgtgatgt
     1741 agtatcattt ctcggcgcga gttatttccg cgccgttgat gatacatatc aatacggttt
     1801 gtcggcccgc ggcctggcga tcgacactta cactgacagt aaagaagagt tccccgactt
     1861 tac
//
LOCUS       DAMZET010000350         1843 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.351, whole genome shotgun sequence.
ACCESSION   DAMZET010000350 DAMZET010000000
VERSION     DAMZET010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.351"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>1843
                     /locus_tag="POB34_004891"
     CDS             <1..>1843
                     /locus_tag="POB34_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDI5921705.1"
                     /translation="STADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVDM
                     SRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINTD
                     ASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVYQ
                     RCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARLD
                     ITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVSE
                     LITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCRELA
                     GVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSYG
                     MFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEYS
                     PAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPIA
                     VRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADRG
                     VDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSG"
BASE COUNT          410 a          457 c          543 g          433 t
ORIGIN      
        1 gttcaaccgc agatgtggcg ctgatcgccc gtcttgccgc gcaggaacgc tattcatcgc
       61 tgatctttac cccccgcggc ggcggcaccg gcactaacgg tcaggcgctc aaccagggga
      121 ttattgttga tatgtcccgc catatgaacc gcatcatcga aattaaccct gaagagggct
      181 gggtgcgcgt tgaggccggg gtgataaaag atcaactcaa tcagtacctg aaaccgttcg
      241 gctacttttt tgcgccggaa ctttcgacca gcaaccgggc aacgctcggt gggatgatca
      301 ataccgatgc atccggtcag ggatcgctgg tctatggcaa aacgtcagat cacgtacttg
      361 gcgtacgcgc ggtgttgttg gggggcgata ttctcgatac gcaaccttta cccgtcgaat
      421 tggcggaaac gctgggtaaa tccaatacca caatcgggcg aatttataac acggtttatc
      481 aacgttgccg tcagcaacgc cagttaatta tcgacaactt ccccaaactt aaccgctttc
      541 ttaccggtta cgatctgcgt catgtcttta acgatgagat gaccgagttc gacctgacgc
      601 gcattctgac gggttcagaa gggacgctgg cctttattac cgaagcgcgg ctggatatta
      661 cgcccttgcc taaagtgcgc cgtctggtga acgtcaaata tgactctttt gactccgcgc
      721 tgcgtaacgc gccgtttatg gttgaggcgc gggcgctttc ggtagagacg gtggactcaa
      781 aagtgctgaa tctggcgcgg gaagatattg tctggcattc cgtcagcgag ttgattaccg
      841 atgtgcctga caaagagatg ctcgggctga acattgtgga gtttgctggt gatgatgagg
      901 cgctgattga cgagcgagtt aatgctctct gtgtgcggct tgatgagctg attgccagcc
      961 aacaggcagg tgtgattggc tggcaggtgt gccgcgagct ggcgggtgtt gaacgtatct
     1021 atgcgatgcg taaaaaagcc gttggtctgc ttggcaatgc caaaggtgcc gctaagccaa
     1081 ttccgtttgc tgaggatacc tgcgtaccgc cggaacatct ggccgattat attgctgaat
     1141 ttcgcgcgct gctcgacagc cacggcttaa gctacggtat gttcggtcac gtcgacgcag
     1201 gtgtcttgca cgtccgtcca gcgctggata tgtgcgatcc tcaacaagag attttgatga
     1261 agcaaatctc tgatgacgta gtggcgctga ctgcgaaata cggtggtttg ttgtggggcg
     1321 agcacggcaa aggttttcgc gctgaataca gcccggcgtt tttcggtgag gaactttttg
     1381 cagaactgcg caaagtgaaa gcggcatttg acccgcataa ccgactcaac ccagggaaga
     1441 tttgcccgcc agaaggtctc gatgcgccga tgatgaaagt ggacgcggtg aagcgcggta
     1501 cattcgatcg gcagatcccc attgcggtac gccagcagtg gcgcggtgcg atggagtgta
     1561 acggcaacgg tttatgcttc aactttgatg cccgtagtcc gatgtgtccg tcgatgaaga
     1621 tcacccagaa ccggattcat tcaccgaaag ggcgcgcaac gctggtgcgt gaatggctgc
     1681 gtttgttggc ggatcgcggc gttgatccac tcaaactgga acaagaactg cctgaatccg
     1741 gcgtcagttt gcggacgtta attgcccgca cgcgcaatag ctggcatgcg aataaaggcg
     1801 aatatgactt ctcacacgaa gtcaaagagg cgatgtcggg ctg
//
LOCUS       DAMZET010000351         1827 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.352, whole genome shotgun sequence.
ACCESSION   DAMZET010000351 DAMZET010000000
VERSION     DAMZET010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.352"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            27..554
                     /locus_tag="POB34_004892"
     CDS             27..554
                     /locus_tag="POB34_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDI5921706.1"
                     /translation="MKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKPPT
                     PDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCTPQ
                     DDSQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDLGE
                     ILAKMKQRTSRGIRK"
     gene            551..1120
                     /locus_tag="POB34_004893"
     CDS             551..1120
                     /locus_tag="POB34_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921707.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKNESYECLIKIQHELIRSEEK"
     gene            1346..1579
                     /locus_tag="POB34_004894"
                     /pseudo
     CDS             1346..1579
                     /locus_tag="POB34_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          618 a          315 c          362 g          532 t
ORIGIN      
        1 ggtatatcac tcaggatccg attgggctga aggggggatg gaatttttat cagtatccgc
       61 tgaatccggt tcagtatata gattcaatgg gactggcatc aaaatatgga cacttaaata
      121 atggcggata tggagcgaga cccaacaaac cgcctacgcc cgatccaagt aaattgccgg
      181 acatagcgaa acaattaaga ctgccatatc ctattgacca ggccagtagt gcgcctaatg
      241 ttttcaaaac attcttcaga gcattaagcc cttacgacta cacactgtat tgcaggaagt
      301 gggtaaaacc aaatctgact tgtacgccac aggatgattc ccagtatcca gggatggata
      361 caaagacagc aagtgattac ctgccacaga caaattggcc aacaactcaa ttaccaccag
      421 gatatacttg tgcagaaccc tatttattcc cagacattaa taaacccgat gggccagcaa
      481 cagcagggat agatgatttg ggtgaaattt tagctaaaat gaaacagaga acatcgagag
      541 gaataagaaa atgaaaagag ttttgttctt tttgctgatg atatttgtta gttttggtgt
      601 tatagctgat tgcgaaatac aagctaaaga tcatgattgt tttactattt tcgctaaggg
      661 tactattttt tccgcgtttc ctgtcttaaa taataaagcc atgtggagat ggtatcagaa
      721 tgaggacatc ggtgagtatt attggcaaac agaactgggt acatgtaaaa ataataaatt
      781 cacaccaagc ggggcgagac tcttaattcg cgtcgggtca ctacgcctta atgaaaatca
      841 tgccatcaaa ggaacgttgc aggagttgat aaatacagca gaaaaaacgg cgtttctcgg
      901 cgatcggttt agaagttata taagagcggg tatctatcaa aaaaagagca gtgatccagt
      961 tcagttgttg gcagtccttg ataattcaat catggtaaaa tattttaaag atgagaaacc
     1021 aacttatgct cgaatgacag cacatctccc gaacaaaaat gagtcttacg aatgtttaat
     1081 aaagatacag catgaactaa ttcgtagtga agagaaataa aatctcttcc tgagaaaaca
     1141 aattaatcat tgtgaaaaat tatataactc atggaatagg tgtttatttt gcgttccatt
     1201 tgcagggaaa gatcacgtaa cgctactttt ttgtactaaa taattcgaat tttaagttca
     1261 acaattgaga tactacttat tgtctaaagc tgtttttcat agcttataca tgatcaaata
     1321 ctccttacat aaataaggtg aacaaatgga acttaaaaaa ttgatggaac atatttctat
     1381 tacccccgat tacagacaag cctggaaagt ggtgcataaa ttgtcagata ttctactgtt
     1441 gactatttgt gccgttattt ctggtgcaga aggttgggaa gatatagagg atttcgggga
     1501 aacacatctc gattttttga agcaatatgg tgattttgaa aatggtattc ctgttcacga
     1561 taccattgcc agagttgtat cccagggaaa gatcacgtaa cgctactttt ttgtactaaa
     1621 taattcgcat tttatgttta aaaattgaga tattccttat tacctaaagc tgtttttcat
     1681 agcttataca tgatcaaata ctccttacat aaataaggtg aacaaatgga acttaaaaaa
     1741 ttgatggaac atatttctat tattcccgat tacagataag cctggaaagt agagcataaa
     1801 ttgtcaggca tcctactatt gactatt
//
LOCUS       DAMZET010000352         1820 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.353, whole genome shotgun sequence.
ACCESSION   DAMZET010000352 DAMZET010000000
VERSION     DAMZET010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.353"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            55..1311
                     /gene="lolE"
                     /locus_tag="POB34_004895"
     CDS             55..1311
                     /gene="lolE"
                     /locus_tag="POB34_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HDI5921708.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLRPVKAVH"
     gene            1326..>1820
                     /locus_tag="POB34_004896"
     CDS             1326..>1820
                     /locus_tag="POB34_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDI5921709.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDAL"
BASE COUNT          403 a          399 c          543 g          475 t
ORIGIN      
        1 ggaaatgcgt gatggacgtc tgacggcgga actgagcctg atgggggcgg agtaatggcg
       61 atgcctttat cgttattgat tggcctgcgt tttagtcgcg gacggcggcg cggcggcatg
      121 gtgtcgctga tctccgttat ttctaccatt ggcattgccc tcggcgtggc ggtattgatc
      181 gtcggcttaa gcgcgatgaa cggctttgaa cgcgaactga ataaccgtat tctggcggtg
      241 gtgccgcatg gtgaaattga ggcggtaaat cagccatgga ctaactggca ggaagcactg
      301 gataacgtgc aaaaagtgcc gggtattgcc gctgctgcgc cgtatatcaa tttcaccgga
      361 ctggtagaaa gtggtgccaa tctgcgcgca attcaggtga agggcgttaa cccgcaacag
      421 gaacagcgtc tgagcgcatt accctcgttt gttcaggggg atgcctggcg caattttaaa
      481 gcgggcgaac agcaaattat catcggcaaa ggcgtggcgg atgcgctgaa agtgaagcag
      541 ggcgattggg tgtcgatcat gatccccaac tcgaatcccg agcataaact gatgcagcca
      601 aaacgtgtgc gtttgcacat tgccggtatt ttgcagttga gtggtcaact cgatcacagt
      661 tttgccatga tcccgctggc ggatgcccaa caatatcttg atatgggttc cagcgtgtca
      721 ggtattgccc ttaaaatgac ggatgttttc aacgccaata agctggtacg cgatgcgggt
      781 gaagtgacca acagctatgt ttatattaaa agttggattg gtacttacgg ctatatgtat
      841 cgcgatatcc agatgatccg cgccattatg tatctggcga tggtactggt gattggcgtg
      901 gcctgtttca acatcgtctc caccttagtg atggcggtga aagacaagag tggcgatatc
      961 gcagtattaa gaacgctggg ggcgaaagat ggtttaattc gcgccatctt tgtctggtat
     1021 ggattgctgg cagggctgtt cggcagcctg tgtggggtga ttatcggtgt ggtggtttca
     1081 ctgcaactta ccccgattat tgagtggatt gaaaagttga tcggtcatca gttcctctcc
     1141 agcgatatct attttattga tttcctgcca tcggaattgc actggctgga cgtcttctac
     1201 gtactggtca cagcattgtt gctgagtctt ttggcaagtt ggtatccggc gcggcgcgcc
     1261 agtaatattg accctgcgcg agtccttagg ccagtaaagg cagtacatta aaacaaggag
     1321 cggcaatgta ttacgggttt gatattggtg gaacaaaaat tgcgcttggc gtgtttgata
     1381 gcggtcggca gttgcagtgg gaaaagcggg tgccgacacc gcgtgacagc tatgacgcat
     1441 ttttagatgc agtgtgtgag ctggtagctg aagctgacca acgttttggc tgtaaaggtt
     1501 ctgtcggcat cggtattccg ggaatgccgg aaacagaaga tggtacgctg tatgccgcca
     1561 atgtccccgc tgccagcggt aaaccgctgc gtgccgacct gagcgcacgt cttgatcgcg
     1621 atgtacgcct tgataacgat gccaactgtt ttgccctttc agaagcctgg gatgatgaat
     1681 ttactcaata tccactggtg atggggttga ttctcggcac cggcgttggc ggcgggctga
     1741 ttttcaacgg caaaccgatt accggtaaaa gctacattac cggcgagttt ggccatatgc
     1801 gtctgccggt tgatgcgtta
//
LOCUS       DAMZET010000353         1756 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.354, whole genome shotgun sequence.
ACCESSION   DAMZET010000353 DAMZET010000000
VERSION     DAMZET010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.354"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..454)
                     /locus_tag="POB34_004897"
     CDS             complement(<1..454)
                     /locus_tag="POB34_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDI5921710.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTS"
     gene            complement(454..1470)
                     /gene="cysP"
                     /locus_tag="POB34_004898"
     CDS             complement(454..1470)
                     /gene="cysP"
                     /locus_tag="POB34_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HDI5921711.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(1641..>1756)
                     /locus_tag="POB34_004899"
     CDS             complement(1641..>1756)
                     /locus_tag="POB34_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI5921712.1"
                     /translation="VGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
BASE COUNT          412 a          473 c          461 g          410 t
ORIGIN      
        1 tgctggtaaa ggccatcgcc accgcaatcc ccagccatgt ataggtgact ttgatatcaa
       61 acttcgccag ccattcaccg taaaaaccgt ttacggaaaa gagcgaggcc agcgttaaac
      121 cggcgacagc cgttggcagc gcaaagggta aatccatcag cgcatcaagc agcgtgcggc
      181 ctgggaagcg atagcgggtt aggatccacg ccatcagcag accgaaaacg ccgttaaaaa
      241 tcgatgccac aaacgccgac agcagcgtta ctttgtaggc cgcgaccacc tgcgggttgg
      301 tgatcacctc ccagtactgc gcccagctca tctgggccag ttgcatcacc agcgcggaga
      361 gcggcagcag caaaatcagg cacacaaaca gcagactggt gccgaggctt aaggtaaagc
      421 ccggcagcac gcgtctggag gagacagcaa acatcagtta cgccccgccg ctaacagctt
      481 gtctaactcg ccgccgctgg tgaagtgggt tttcatcact tccggccagg agccaaattt
      541 gtcttccacg cggaacagct cggtctgcgg gaatttgtct ttcagtttgt ccatcacctc
      601 cgggttattc acgcggtagt aatagtcggt gatgatggtt tgcgcctgcg ggctatagag
      661 ccagttcaga taggctttgg cggctttttc cgtaccgttg gcctgcacgt ttttatcaac
      721 ccacgccacc gggaattccg ccagaatgtt ggttttcgga atcaccactt caaagccctg
      781 cgcttcatac tgtttacgga tgttgttcac ttccgattcg aagctaatca gcacatcgcc
      841 caggccacgc tcggcaaaag tggtggttgc gccacgaccg ccggtatcga acacttcaac
      901 gtttttcagg aactgggtca taaactgttc ggttttggct ttatcgccac cgtcagcttt
      961 atccgctgcg ccccatgccg ccagataggt ataacgcgcg ttacccgacg ttttcgggtt
     1021 cgggaaaatc agcttcacgt cggagcgcac caggtcgttc caatcgtgga tattcttcgg
     1081 gttaccctta cgcaccagga agcccatggt ggagtagaac ggcgagctat tattcggcag
     1141 gcgcgactgc cagtcggccg ggatcagctt gcctttatcg tgcaggattt gtacgtcggt
     1201 cacctggtta taagtgacaa cgtcggcttt taagccctgt aaaatcgcca gcgcctgttt
     1261 tgatgacccg gcatgagatt gttttatcgt cagtttgtcg ccgccgttat cttttgccca
     1321 ttgttgctca aacggcggat tcagggcggc aaacagctcg cgggagacgt cataagaact
     1381 gttcagcagt tccgttgcct gtacatggcc cgccagcagc agagaagcga ccagcgcgag
     1441 tgagttcttt ttcagtaagt taacggccat tgcgcaccct tataaattta atgactttct
     1501 aatagccatc atatttataa cggacgtgaa aggagtaacg gttttatata ccgtttggtg
     1561 atttggaagt tgaaaaggga ataagaaccg ggtttaaaac gaaatcctcc ccacaaatgc
     1621 agggaggaaa cagaggtgaa tcagataccg acgctaaccg tctccggcag tgtgctgccg
     1681 ccatcaatca cattctgtgt accggttaaa tagctggatt catccgatgc gaggaaggcc
     1741 gccagttcgc cgactt
//
LOCUS       DAMZET010000354         1742 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.355, whole genome shotgun sequence.
ACCESSION   DAMZET010000354 DAMZET010000000
VERSION     DAMZET010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.355"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            14..847
                     /locus_tag="POB34_004900"
                     /pseudo
     CDS             14..847
                     /locus_tag="POB34_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
     gene            950..1246
                     /locus_tag="POB34_004901"
                     /pseudo
     CDS             950..1246
                     /locus_tag="POB34_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            1246..>1742
                     /locus_tag="POB34_004902"
     CDS             1246..>1742
                     /locus_tag="POB34_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDI5921713.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSSY
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNNLMQVASEHIAPLQDAADLEIATEEE
                     ISLLEA"
BASE COUNT          474 a          405 c          471 g          392 t
ORIGIN      
        1 aaaggaggca gcagtgccgc aacgaaacga caggagccgc agcacaccga cgaccagtcc
       61 gaagaataat tcctggttca ggatgcaggc aggtcaccag agtgacgcgg atatttatat
      121 ttatgacgag attggtttct ggggtgttac ggcgaagcag tttatcagtg atctgaatgc
      181 actgggcgat atcacccaca ttaatctcca tattaattca ccgggtggcg atgtctttga
      241 aggcatcgcc atttttaatg cactgaaaac acatggtgcg tccattaccg tttatgtcga
      301 cggtgtggcg gcgtcaatgg cgtcggtcat tgcgatggtg ggaaacccgg tcattatgcc
      361 ggaaaactcc ttcatgatga ttcataaacc atttggattt acgggcggtg atgcggagga
      421 catgcgcacc tatgccgacc tgctcgataa agttgaggcg gttctgttac ccgcttatgc
      481 acagaaaacc gggaaaacca ccgatgaaat tgctgccatg ctggcggatg agacctggat
      541 gtccggtgcc gaatgtctgg cacagggatt tgctgatcag gtgacaccag ccgttaaggc
      601 aatggcatgt attcagtcaa aacgtacaga ggaatttaaa aagatgccgg aatccattcg
      661 aaacatgatt actccgccac gcaacagtgc tccacgcgta caggatgatg aacctgcagc
      721 ctcccggacg ccagtgcagg cagcagcacc cgtggtggat gaaaacagta tccgtgcgca
      781 ggtactggca gagcaaaaag cgcgtgcaaa cggtattaat gatctgtttg ccatgtttgg
      841 cgggcgttag ccgcgctgac agacaatacg cagggggcag caggtcttga gttatacgag
      901 gtgtataaca acggatatcc aacagcgtat ggaaatatca ttcacctgaa accatactca
      961 tagtgtcaca ggcgcatcag caatcagcca gtggtcacaa aatgggtcag tacataaggt
     1021 agtgtctgcg gccagtgtga atacaagtgc tgcaggagcg cacactcata gtgtcagcgg
     1081 cacagctgca tctgcaggtg ctcacgcaca tactgtcggt attggtgctc atacgcactc
     1141 tgttgcgatt ggctcacatg gacacaccat caccgttaac gctgctggta acgcggaaaa
     1201 caccgtcaaa aacatcgcat ttaactacat tgtgaggctt gcataatggc attcagaatg
     1261 agtgaacaac cacggaccat aaaaatttat aatctgctgg ccggaactaa tgaatttatt
     1321 ggtgaaggtg acgcatatat tccgcctcat acaggtctgc cagcaaacag ttcctatatt
     1381 gcaccgccag atattccggc tggcttcgtg gctgttttca acagtgatga ggcatcgtgg
     1441 catctcgttg aagatcatcg gggtaaaacc gtctatgacg tggcttccgg cgacgcgtta
     1501 tttatttctg aacttggccc attaccggaa aatgtcacct ggttatcccc ggaaggggag
     1561 tttcagaagt ggaacggcac agcctgggtg aaggatacgg aagcagaaaa actgttccgg
     1621 atccgggagg cggaagaaac aaaaaacaac ctgatgcagg tagccagtga gcatattgcg
     1681 ccgcttcagg atgctgcaga tctggaaatt gcaacggagg aagaaatctc gttgctggaa
     1741 gc
//
LOCUS       DAMZET010000355         1739 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.356, whole genome shotgun sequence.
ACCESSION   DAMZET010000355 DAMZET010000000
VERSION     DAMZET010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.356"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..805
                     /gene="xylF"
                     /locus_tag="POB34_004903"
     CDS             <1..805
                     /gene="xylF"
                     /locus_tag="POB34_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI5921714.1"
                     /translation="NGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEAKQEGI
                     KVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNNAKLFR
                     AGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVASNDATA
                     GGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAEIAVEL
                     GNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            883..>1739
                     /locus_tag="POB34_004904"
     CDS             883..>1739
                     /locus_tag="POB34_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI5921715.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKR"
BASE COUNT          512 a          369 c          428 g          430 t
ORIGIN      
        1 aaatggcaat gaagaaacac aaatgtcgca gattgaaaac atgattaacc ggggtgtcga
       61 tgttcttgtc attattccgt ataacggtca ggtattaagt aacgttgtaa aagaagccaa
      121 acaagaaggc attaaagtac tagcttacga ccgtatgatt aacgatgcgg atatcgattt
      181 ttatatttct ttcgataacg aaaaagtcgg tgaactgcag gcaaaagccc tggtcgatat
      241 tgttccgcaa ggtaattact tcctgatggg cggctcgccg gtagataaca acgccaagct
      301 gttccgcgcc ggacaaatga aagtgttaaa accttacgtt gattccggaa aaattaaagt
      361 cgttggtgac caatgggttg atggctggtt accggaaaac gcattgaaaa ttatggaaaa
      421 cgcgctaacc gccaataata acaaaattga tgctgtagtt gcctcaaacg atgccaccgc
      481 aggtggggca attcaggcat taagcgcgca aggtttatca gggaaagtag caatttccgg
      541 ccaggatgcg gatctcgcag gtattaaacg tattgctgcc ggtacgcaaa ctatgacggt
      601 gtataaacct attacattgc tggcaaatac tgccgcagaa attgccgttg agttgggcaa
      661 tggtcaggaa ccaaaagcag ataccacact gaataatggc ctgaaagatg tgccctcccg
      721 cctcctgaca ccgatcgatg tgaataaaaa caacatcaaa gacacggtag ttaaagacgg
      781 attccacaaa gagagcgagc tgtaagcgtc acgccccggc gcggagcggg ggcgtgattt
      841 ctctccatgc cgcgtgaatg aattggctta ggtggagtcg ttatgcctta tctacttgaa
      901 atgaagaaca ttaccaaaac cttcggcagt gtgaaggcga ttgataacgt ctgcttgcgg
      961 ttgaatgctg gcgaaatcgt ctcactttgt ggggaaaatg ggtctggtaa atcaacgctg
     1021 atgaaagtgc tgtgtggtat ttatccccat ggctcctacg aaggcgaaat tatttttgcg
     1081 ggagaagaga ttcaggcgag tcacatccgc gataccgaac gcaaaggtat cgccatcatt
     1141 catcaggaat tggccctggt gaaagaattg accgtgctgg aaaatatctt cctgggtaac
     1201 gaaataaccc acaatggcat tatggattat gacctgatga cgctacgctg tcagaagctg
     1261 ctcgcacagg tcagtttatc catttcacct gatacccgcg ttggcgattt agggcttggg
     1321 caacaacaac tggttgaaat tgccaaggca cttaataaac aggtgcgctt gttaattctc
     1381 gatgaaccga cagcctcatt aactgagcag gaaacgtcgg ttttactgga tattattcgc
     1441 gatctacaac agcacggtat cgcctgtatt tatatttcgc acaaactcaa cgaagtcaaa
     1501 gcgatttccg atacgatttg cgttattcgc gacggacagc acattggtac gcgtgatgct
     1561 gccggaatga gtgaagacga tattatcacc atgatggtcg ggcgagagtt aaccgcgctt
     1621 taccctaatg aaccacatac caccggagat gaaatattac gtattgaaca tctgacggca
     1681 tggcatccgg ttaatcgtca tattaaaaga gttaatgatg tctcgttttc cctgaaacg
//
LOCUS       DAMZET010000356         1737 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.357, whole genome shotgun sequence.
ACCESSION   DAMZET010000356 DAMZET010000000
VERSION     DAMZET010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.357"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(143..376)
                     /gene="ydfK"
                     /locus_tag="POB34_004905"
     CDS             complement(143..376)
                     /gene="ydfK"
                     /locus_tag="POB34_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HDI5921716.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVLKDNQNLNGKM"
     gene            complement(445..558)
                     /locus_tag="POB34_004906"
     CDS             complement(445..558)
                     /locus_tag="POB34_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921717.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
     gene            1160..1737
                     /gene="ydfJ"
                     /locus_tag="POB34_004907"
                     /pseudo
     CDS             1160..1737
                     /gene="ydfJ"
                     /locus_tag="POB34_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
BASE COUNT          482 a          284 c          415 g          556 t
ORIGIN      
        1 tgtttttcga gttccgggcg gactcaagga agaagaatag tgttgcgtgt tattttaacc
       61 agatttcaag ttgtttggtc gtggaaaagt ggagcaaaat gttgttaaag tggaaaaatg
      121 ataaaaaagt aagtttatta tattacattt taccatttaa attttggttg tctttaagaa
      181 ctgatatcgc tgtttgtaat aattctttgt tatccagcca tgattttttc tttatgtttc
      241 cttcaatgta atcaagcaat gttctggtat tgataggtct tccctgtttt gctacttcca
      301 ctacagcatc ccctaggata attcttactt caggaagctg cgcagggaac cactttaggg
      361 tgtcttttga tttcatgaag atattcctta aaatattatt gattttcatt gcgatattgt
      421 atgtctgatt caggatatgt tggcttatac atcggttttg tctgggttat tggatatgcc
      481 aatccctaat tttattaggg catgactaaa aatgctgaat atgataagga gagaagtgat
      541 tatcagtatg ctgttcatat agcctcgaat tagtaatgtg ttatatatga tatggttgac
      601 aatttttatc ttgggtgttc ttaaagttcg tagataaaca ttgtcgtttc aggtatacag
      661 gaatgctaac aggtggcagc aaaaatcagg cggtttatgg cgcaagctga agcggcaact
      721 gcaaactatc ttatgcagag actctacacg gattgggttt aaaagtatac atagataaca
      781 atttttatct gaagaagaaa aatatcaagg tgatatagcc tatatgcctt tgatgcggag
      841 gaatgaatgt gatgggagtg atgtatctga atagttgaaa aaccgcagac acgccttatg
      901 caagaacgtg ctgcggttgg ctggtaaatt tttcgatagt gcgagtattg aatgatttcc
      961 agccgttatt gattttacgc gtaaatccat gaaaaactac ttatctgttg ggagtctttt
     1021 tggggcatat atgggacaga aataggcccc agatagacac tgagatcgaa cttaggatgc
     1081 tttttaaaaa atgcaactat ctgaaaaaac ctagaaaacg ccaaggaaac cacaggatgg
     1141 gaaaaaacac ctgtgaatta tggatttcca gttatattcg ctcggcgcag cgttagtgtt
     1201 tcatgaaata ttttttcctg aatcatcaac ggcaatggcg ttaattttgg caatgggaac
     1261 ctacggtgca ggttatgtgg cgcgtattgt cggagcattt attttcggca aaatgggcga
     1321 cagaataggg cgtaaaaaag tgctctttat taccatcacc atgatgggga tctgtaccac
     1381 cttaattggt gtgttaccga cctatgcaca gattggtgtt tttgccccca tcttgctggt
     1441 gacgctgcgt attattcagg ggttgggtgc aggtgcggaa atttccggtg ccggtacgat
     1501 gctggcggaa tatgcaccaa aaggtaagcg cggaattatc tcctcatttg tagctatggg
     1561 aactaactgc ggaaccttga gcgcaacggc aatctgggcc tttatgttct tcattctcag
     1621 taaagaggaa ctgctggcgt ggggatggcg tataccgttc ctggcgagcg ttgtcgtgat
     1681 ggtctttgct atctggttac gtatgaatct gaaagaaagc ccggtctttg agaaggt
//
LOCUS       DAMZET010000357         1683 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.358, whole genome shotgun sequence.
ACCESSION   DAMZET010000357 DAMZET010000000
VERSION     DAMZET010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.358"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(122..1372)
                     /gene="icd"
                     /locus_tag="POB34_004908"
     CDS             complement(122..1372)
                     /gene="icd"
                     /locus_tag="POB34_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDI5921718.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1544..>1683
                     /locus_tag="POB34_004909"
     CDS             1544..>1683
                     /locus_tag="POB34_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="catalyzes the formation of pseudouridine from
                     uracil-2457 in 23S ribosomal RNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridylate synthase"
                     /protein_id="HDI5921719.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKP"
BASE COUNT          412 a          429 c          375 g          467 t
ORIGIN      
        1 cccatatggg ctcaagtaaa gggttttata taaatatgct cgatatgcta ttaataatac
       61 ctacataaat aaaacaacgg gcgtgttata cgcccgttgc aatattaaac acatgtagtg
      121 attacatgtt cttgatgatc gcatcaccaa attctgaaca tttcagcagt ttagcgcctt
      181 ccatcagacg ttcgaagtca taggttacgg tcttggcatt gattgcgcct tccatacctt
      241 taacaatcag gtctgcggct tcggtccaac ccatgtggcg taacatcatc tcagcagaga
      301 gaataataga gcctgggttt actttgtcct gaccggcata tttcggcgca gtaccgtggg
      361 tggcttcaaa cagggcgcat tcgtcaccga tgtttgcacc tggggcgata ccgataccgc
      421 caacctgcgc tgccagggcg tcagaaatgt agtcaccgtt caggttcata caggcgataa
      481 catcatattc agccggacgc agcaggatct gttgcaggaa tgcatcagca atcacgtctt
      541 taatgacgat ctctttgccg gtgttcgggt ttttaacttt cagccacggg ccgccgtcga
      601 tcagttcacc gccaaactct tcacgcgcca gctggtagcc ccagtcttta aacgctcctt
      661 cggtgaactt catgatgttg cctttgtgca ccagagtcac agagtcacga tcgttagcaa
      721 ttgcgtattc gatcgctgca cgaaccagac gtttggtgcc ttcttccgaa cacggcttaa
      781 taccgatacc acaatgttcc gggaagcgaa ttttcttcac ccccatctct tcacgcagga
      841 atttaatcac tttctcggcg tcggcagagt ctgctttcca ttcgataccc gcataaatgt
      901 cttccgagtt ttcacggaag ataaccatat cggtcagttc agggtgttta accgggcttg
      961 gagtgccctg atagtaacgt accggacgca ggcagatgta gagatccagt tcctggcgca
     1021 gggcaacgtt cagagagcga ataccgccac caacaggagt ggtcagcgga cctttaatgg
     1081 caacgcgata ttcacgaatc agatcaaggg tttcagcagg cagccagaca tcctgaccat
     1141 aaacctgtgt ggatttttca ccggtgtaaa tttccatcca ggagatttta cgctcgcctt
     1201 tataggcttt ctcgactgca gcgtcgacca ctttcagcat ggctggggtt acatctacac
     1261 cgattccatc accttcaatg taagggataa tcggattttc aggaacgttg agtttgccgt
     1321 tttgcagggt gatcttcttg ccttgtgccg gaacaactac tttactttcc attcacctct
     1381 ccttcgagcg ctactggttt gctcgtctgc caccacgttg catatgcgtt tgcgtcctgc
     1441 gatacggatg ctttagagca attttttgtt aatgatttgt aattggcttg tcaatactac
     1501 cctattgttt gccgtcatga aagatgcgcg ttattaggct ataatgcggc aattcataat
     1561 ctctgaaaat accatgcaaa aaacttcttt tagaaatcac caggttaagc gattcagctc
     1621 gcaacgttct accaggcgta aacctgaaaa ccagcccacg cgtgtgatcc tgttcaataa
     1681 acc
//
LOCUS       DAMZET010000358         1675 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.359, whole genome shotgun sequence.
ACCESSION   DAMZET010000358 DAMZET010000000
VERSION     DAMZET010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.359"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..910)
                     /gene="kdpA"
                     /locus_tag="POB34_004910"
     CDS             complement(<1..910)
                     /gene="kdpA"
                     /locus_tag="POB34_004910"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit A"
                     /protein_id="HDI5921720.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEV"
     gene            complement(910..999)
                     /gene="kdpF"
                     /locus_tag="POB34_004911"
     CDS             complement(910..999)
                     /gene="kdpF"
                     /locus_tag="POB34_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDI5921721.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            1312..1518
                     /locus_tag="POB34_004912"
     CDS             1312..1518
                     /locus_tag="POB34_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HDI5921722.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
BASE COUNT          495 a          409 c          399 g          372 t
ORIGIN      
        1 gaacttctgc ccacatcacc acgcctacgc agatgacaaa aatcactgac atcgcccaca
       61 gcaacatgcg cccctggcgg cgatcgcccg tcacttcacc aaaggcaaag cacagcgccg
      121 ttgggatcaa gaagatcgcc agcatctgca cgaagttggt cagtgcggtt gggttttcaa
      181 acggatgcga cgagttggca ttaaagaagc caccgccgtt agtaccgagc atcttgatcg
      241 cttcctgaga agctacaggc cccatgggta acagctgttg cgctccttca acggtattca
      301 cagcctgata aggctgaaag ttttgcagcg caccttgttg aataaaaaac agtgcgatca
      361 acaacgccac agggacgagc acccataacg tgatgcgtag cagatcgacc caggcattcc
      421 cgagtgtgct catgctctgg cgggtaaacg cacggatgag ggcaaaaatc accgcaatcc
      481 cgctggcggc agaaagaaag ttttgcaccg ttaagccagc catctggctg aaatagctca
      541 acgtggtttc accgctgtaa gattgccagt tggtattcgt gacaaagctg acggcggtat
      601 tcagcgccag atcccacgac agccccggca actgctgtgg attaagcggc agatagtgct
      661 gaccgagcaa cataaaaaac agcaccgcca gccccagcat gttcaggccg agaatggcac
      721 agagatattg cttccagttc atctcacggt cagagacgcc aagtgcgcga aaaagtacgc
      781 gctcaacgcc cgttgtaccg ggaagaggaa tgtcattaat cagccgcgcc agcccgctgc
      841 ctaaaggacg cgccagcacc attaacacca gtaaaaacgt ggcgatcagt aagaaccctt
      901 gcgcagccat cagaacgcct ccgcattgat cagggcataa accagataac ccagtaataa
      961 aaacaccagc aatacgccgg ttatcacgcc tgcactcaca gtgcacctcc agtggcctaa
     1021 aagtgatacc ggaagggtag aattctggct gcaaagattt cgcaaaaatc tgcgggcggg
     1081 gtgtaaaaaa agtataaaaa tggcaaaagc catgatttaa ctaatgtttg gtattaattt
     1141 aacttttgtg taacttaatt acaggatgaa tgtaaataaa ccatcaataa gcaaaaataa
     1201 gtggtcggat gagtagtaaa attacacaaa aggcggtact attttcatca gataaacaaa
     1261 ttcatttttc cggtgccatt caccgggtag atacaaaagg gggagaaaag tatggaactc
     1321 tacagagaat atcctgcatg gcttatcttt ttacgccgta cttatgcggt tgcagcgggc
     1381 gttctggcgc tgcctttcat gcttttctgg aaagaccgcg cccgctttta cagctacctg
     1441 catcgcgtct ggtcgaaaac cagcgataaa ccggtgtgga tggatcaggc cgaaaaggca
     1501 actggtgatt tttattgatt gctgagtttt gaatacattg aaaaccgcca gcggaaacgc
     1561 tggcggtttt ttgtatcagg aagatgttca gaggaatatt tagacatcgt tataccaatc
     1621 gaaacatata ttcatgaaat atatataaat attttcctaa ttgttcttat ctgac
//
LOCUS       DAMZET010000359         1665 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.360, whole genome shotgun sequence.
ACCESSION   DAMZET010000359 DAMZET010000000
VERSION     DAMZET010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.360"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..181
                     /locus_tag="POB34_004913"
     CDS             <1..181
                     /locus_tag="POB34_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001700483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDI5921723.1"
                     /translation="FSSSGEEIEPPDSVTFHIWTAYSPFTTWVQIVKDWMKTKGDNRI
                     PSRQGAAGKSRPDGG"
     gene            123..1058
                     /locus_tag="POB34_004914"
                     /pseudo
     CDS             123..1058
                     /locus_tag="POB34_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
     gene            1125..>1665
                     /locus_tag="POB34_004915"
     CDS             1125..>1665
                     /locus_tag="POB34_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HDI5921724.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYIRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEG"
BASE COUNT          436 a          391 c          465 g          373 t
ORIGIN      
        1 gttttcgtca tccggtgaag agattgaacc gcctgacagt gtgacctttc acatctggac
       61 ggcgtacagc ccgttcacca cctgggtgca gattgtcaaa gactggatga agacgaaagg
      121 ggataaccga atcccatctc ggcaaggagc tgctggaaaa agtcgacctg acggaggata
      181 acgccagcag actggaggag ttttcgaaag agtggaaaga cgccaacgat aaatggaatg
      241 ccatgtgggg cgtcaaaatt gagcagaccg aagacggtaa gcattatgtc gcggggcttg
      301 gcctcagcat ggaggataca gaggaaggca aactgagcca gtttctggtt gctgctaacc
      361 gtatcgcgtt tattgacccg gcaaacggga atgaaacgcc gatgtttgtg gcgcagggca
      421 accagatatt catgaacgac gtgttcctga aacgcctgac ggcccccacc attaccagcg
      481 gtggcagtcc tccggtattt tccctgacat cagacggaaa gctgaccgct aaaaatgcgg
      541 atatcagtgg cagtgtgaat gcgaactccg ggacgctcaa caacgtcacg attaatgaga
      601 actgtcagat taaggggaaa ctgtcagcca atcagattga aggcgatatt gtcaaaacgg
      661 tcagcaagtc tttcccccgc acgaacagtt atgccagtgg caccatcacg gtaagaatca
      721 gtgatgatca gaaatttgac cggcaggtca tgataccgcc agtgttattc cgcggtggta
      781 agcatgagaa tttcaacagt aataaccaac agtcatactg gtattcaacc tgccggttaa
      841 gagtgaccct caatggtcag gagattttta atcagtccac gacggatgct cagggcgtat
      901 tttcctccgt tatagatatg cctgccggac aggggacgct gacactgaca ttcaccgtat
      961 cttcatcagg agcgaataac tggacaccaa caaccagtat cagcgatctg ctggttgtgg
     1021 tgatgaaaaa atccacagca ggtatcagta tcagctgaat tttataaccc agaacgggcg
     1081 tcagaaatga cgcctttttt attgcagaaa agcgagaggt aattatgcgt aaactttatg
     1141 ccgccatttt gtccgcagcc atttgtctgg ccgtatccgg tgcgcctgca tgggcgtctg
     1201 aacatcagtc cacgctgagc gcggggtatc ttcatgcccg gacgaacgtt cccggcagtg
     1261 atgatctgaa cgggattaac gtgaaatacc gttatgagtt tacggacaca ctggggatgg
     1321 tgacgtcatt cagctatgca ggagacaaga atcgtcagct gacccgttac agcgataccc
     1381 gctggcatga agattccgtt cgtaaccgct ggttcagcgt aatggcgggg ccgtctgtgc
     1441 gcgtgaatga atggttcagc gcgtatgcga tggcgggtgt ggcttacagc cgtgtgtcga
     1501 ctttttccgg ggattatatc cgcgtaactg acaataaggg gaaaacgcac gatgtgctga
     1561 ccggaagtga tgacggtcgc cacagcaaca cgtctctggc gtggggggct ggcgtgcagt
     1621 ttaacccgac cgaatccgtg gccattgata ttgcttatga aggct
//
LOCUS       DAMZET010000360         1660 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.361, whole genome shotgun sequence.
ACCESSION   DAMZET010000360 DAMZET010000000
VERSION     DAMZET010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.361"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..341)
                     /locus_tag="POB34_004916"
     CDS             complement(<1..341)
                     /locus_tag="POB34_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HDI5921725.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKS"
     gene            496..1494
                     /locus_tag="POB34_004917"
     CDS             496..1494
                     /locus_tag="POB34_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI5921726.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
BASE COUNT          430 a          388 c          408 g          434 t
ORIGIN      
        1 ttcgatttaa aacgtagcgc caggcggatt tcactgacgc cgcgcgttgg cgtcggccag
       61 gcgggaggat cgccatataa ctgcgccagt ccttcatcat atgtgaagcg cggaatgcca
      121 aagtcacgct gaggaatgcg ttttacgccc agcaggcgct cttcacgcac cgcgcttaac
      181 agcggcaagc gtgctccggc gtgaatattg agcaactgat tgaccatcgc agtgataaac
      241 gctgttttgc cgctgcggct tagtccggtt acggcgaggc gcagatgtct gtcgacaccc
      301 cgattcacca gcgcattaag ttcattttta agtcgcttca ttgctgtcct gtgctgctct
      361 gaagaccaga aaaaaatcat tcgtaaggat atgaatgtgg gggccaggcc attgattatc
      421 aactgcccgt gttgtcatcg acgggcgaac tccataagat gtgcagcatt cacgttcgga
      481 cggagttcct gacctatgtc ccctactatt tatgatattg ccagggttgc aggcgtatca
      541 aaatccaccg tatcacgcgt gctgaataag caaactaata tctccccgga agcgcgcgaa
      601 aaagtgttac gggccattga agaattacag tatcagccaa acaagctggc ccgcgcgctg
      661 acctcttcgg gttttgatgc cattatggtg atttctaccc gttcgaccaa aactacggcg
      721 ggtaatccgt ttttctctga agttttgcat gccatcaccg ccaaagctga agaggaaggt
      781 ttcgacgtga tattgcagac gtcgcacaat ccggcagaag acttacaaaa atgtgaaagc
      841 aaaattaagc agaaaatgat taaaggcatt attatgctaa gttcgccagc ggatgagtca
      901 ttttttgccc aacttgataa atacgatatt cctgtcgtgg tgattggcaa agttgaaggt
      961 caatatgccc atgtttattc tgtcgatacc gataattttg gcgacagcat tgcgttgacc
     1021 gatgcattaa ttgaaagtgg gcatcaaaat attgcctgcc tgcatgctcc gcttgatgtc
     1081 catgtttcag tggatcgggt aaatggttat aagcagagcc tggctgcgca taatattgca
     1141 gtgcgtgatg aatggattgt tgacggcggt tatacccatg aaacagcact gcaagccgcg
     1201 cgggaattat taagccagtc accgttgcct gaggcagtgt ttgctactga cagcctgaaa
     1261 ttaatgagta tttatcgtgc ggcagcagag aaaaatattg ctattccgca gcagttagcg
     1321 gtggtgggtt atagtaatga aacgctgtcg tttattttaa cgcctgcgcc aggcggtatt
     1381 gatatcccaa cacaggagtt aggtcaacaa agctgtgagt tattattccg cttaatttct
     1441 ggaaaaccgt caccacagaa tattaccgtt gccacgcata tgacgctgaa ataaatacca
     1501 gctaccttgc ctgaatgccg gatgcgatgc tagcgcatct tatccggcct acatttatag
     1561 ttcggataaa gtactcgtgc cgcatccggc aatttagccc attatcagaa cgagtaattt
     1621 acgccgacac ctgcataatg gaatttatcg ctgtcgcctt
//
LOCUS       DAMZET010000361         1612 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.362, whole genome shotgun sequence.
ACCESSION   DAMZET010000361 DAMZET010000000
VERSION     DAMZET010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.362"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(5..>1612)
                     /gene="mdoH"
                     /locus_tag="POB34_004918"
     CDS             complement(5..>1612)
                     /gene="mdoH"
                     /locus_tag="POB34_004918"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HDI5921727.1"
                     /translation="FYRRRRRRVKRKSGNIDDFCRRWGSQYSYMVVLDADSVMTGDCL
                     CGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFATRVYGPLFTAGLHFWQLGESHY
                     WGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDFVEAALMRRAGWGVWIAYDLPGS
                     YEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHPVHRAVFLTGVMSYLSAPLWFMF
                     LALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPELAIALFASTMVLLFLPKLLSILL
                     IWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLFHTVFVVSAFLGWEVVWNSPQRD
                     DDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLFWLAPIVFSLILSPFVSVISSRA
                     TVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQRSLDDGFMHAVFNPSFNALATA
                     MATARHRASKVLEIARDRHVEQALNETPEKLNRDRRLVLLSDPVTMARLHFRVWNSPE
                     RYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT          423 a          475 c          428 g          286 t
ORIGIN      
        1 gtttttattg cgaagccgca tccggtttac gcaatgccag tggattgagc tttatccctt
       61 cgtaataact cacccatgaa gaatatctct ccggggaatt ccagacgcgg aaatgcagac
      121 gggccatcgt caccggatcg cttagcagca ccaggcgacg atcgcgattc agcttctctg
      181 gcgtctcgtt cagcgcctgt tcaacgtggc ggtcacgggc gatttccagc accttactgg
      241 cgcggtgacg cgcggtcgcc attgcggttg ccagagcgtt aaatgacggg ttaaacactg
      301 cgtgcataaa gccatcatca agggagcgtt gacgattcat ctcaaggaac cgatcggtat
      361 caaccagcac ctgcggcggc gaatactctt ccgggatcag gaacagtttc cagcgtttgg
      421 tgcgcagacc aacggtggca cggctggaaa tcaccgaaac aaacggtgac aggatcaacg
      481 agaagacaat cggtgccagc cagaacagga aacgcagatc caaccacgcc ataccaacag
      541 cccacactaa ccctaacagc agctgtgagc cgtggcgttt gaacgcttca ccccaggaag
      601 tggagtcatc atcacgctgc ggtgaattcc acaccacttc ccagccaagg aacgcgctga
      661 cgacgaagac cgtatggaac agcatgcgta ccggagccag cagcacggaa aacagcactt
      721 ccagcagcaa cgataatgta acgcgccaga agccgccgta ttctttcgtt cctttgcacc
      781 agataagcaa aatgctcaat aacttcggca ggaacaacag caccatggtc gaagcaaaaa
      841 gtgcaatcgc cagctcagga cgccactgcg gccacaccgg gaacaactgc cgtggttgca
      901 ggaagtattg cggttcggtc aacgcatgta ctacctgcaa tgcagtagag agcgcgagga
      961 acataaacca cagcggagcg gagagataag acatcacgcc cgtcaggaat accgcacggt
     1021 gaaccgggtg catacccttc accaggaaca gacggaagtt catcaggtta ccgtggcacc
     1081 agcggcggtc acgttttagc tcatcaagca agttaggcgg caattcttca taagaacccg
     1141 ggagatcgta agcaatccag accccccaac ctgcacggcg catcaacgcc gcttccacga
     1201 agtcatgtga caggattgaa ccggcaaagg aaccttcgcc cggcagcgga gccagtgcgc
     1261 agtgctcgat aaacggtttc acgcggataa tcgcgttatg tccccagtag tgcgactcgc
     1321 caagttgcca gaagtgcaaa ccggctgtaa acagtggccc atacacgcgg gtcgcgaact
     1381 gctgacagcg cgcatacagc gtatccatac cggacgcttt cggcgacgac tgaatgatcc
     1441 cggcgttcgg gttggcttcc atcaggcgca ccagcccgca caaacaatca ccggtcatta
     1501 ccgagtcagc atccagcacc accatgtagc tgtactggct gccccagcga cggcagaagt
     1561 catcgatatt accgctttta cgcttcacgc ggcgacggcg gcggcgatag aa
//
LOCUS       DAMZET010000362         1597 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.363, whole genome shotgun sequence.
ACCESSION   DAMZET010000362 DAMZET010000000
VERSION     DAMZET010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.363"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>1597
                     /gene="fdnG"
                     /locus_tag="POB34_004919"
     CDS             <1..>1597
                     /gene="fdnG"
                     /locus_tag="POB34_004919"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_except=(pos:237..239,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDI5921728.1"
                     /translation="SDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLST
                     GMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWVD
                     IKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIRS
                     GTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYDK
                     SSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCEV
                     LASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSNI
                     QGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSLM
                     KSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDKN
                     KVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDGS
                     IANSGRWLQWHWKGQDAPGEARN"
BASE COUNT          383 a          416 c          441 g          357 t
ORIGIN      
        1 gttctgacaa atggcagcgc attagctggg aagaagcatt ctcccgtatt gcgaagctga
       61 tgaaagctga ccgtgacgct aactttattg aaaagaacga gcagggcgta acggtaaacc
      121 gttggctttc taccggtatg ctgtgtgcct ccggtgccag caacgaaacc gggatgctga
      181 cccagaaatt tgcccgctcc ctcgggatgc tggcggtaga caaccaggcg cgcgtctgac
      241 acggaccaac ggtagcaagt cttgctccaa catttggtcg cggtgcgatg accaaccact
      301 gggtggatat caaaaacgct aacgtcgtga tggtgatggg cggtaacgct gctgaagcgc
      361 atcccgtcgg tttccgctgg gcgatggaag cgaaaaacaa caacgatgca accttgatcg
      421 ttgtcgatcc ccgttttacg cgtaccgctt ctgtggcgga tatttacgcg cctattcgtt
      481 ccggtacgga cattacgttc ctgtctggcg ttttgcgcta cctgatcgaa aacaacaaaa
      541 tcaacgccga atacgttaag cattacacca acgccagcct gctggtgcgt gatgattttg
      601 ctttcgaaga cggtctgttc agcggctacg acgctgaaaa acgtcaatac gataaatcgt
      661 cctggaacta tcagttcgat gaaaacggct atgcgaaacg cgatgaaaca ctgactcatc
      721 cgcgctgtgt gtggaacctg ctgaaagagc acgtttcccg ctacacgccg gacgtcgttg
      781 aaaacatctg cggtacgcca aaagccgact tcctgaaagt gtgtgaagtg ctggcctcca
      841 ccagcgcacc ggatcgcaca accaccttcc tgtacgcgct gggctggacg cagcacactg
      901 tgggtgcgca gaacatccgt actatggcga tgatccagtt gctgctcggt aacatgggta
      961 tggccggtgg cggcgtgaac gcattgcgtg gtcactccaa cattcagggc ttgactgact
     1021 taggcctgct ctctaccagc ctgccaggtt atctgacgct gccgtcagaa aaacaggttg
     1081 atttgcagtc gtatctggaa gcgaacacgc cgaaagcgac gctggctgat caggtgaact
     1141 actggagcaa ctatccgaag ttcttcgtta gcctgatgaa atctttctat ggcgatgccg
     1201 cgcagaaaga gaacaactgg ggctatgact ggctgccgaa gtgggaccag acctacgacg
     1261 tcatcaagta tttcaacatg atggatgaag gcaaagtcac cggttatttc tgccagggct
     1321 ttaacccggt tgcgtccttc ccggacaaaa acaaagtggt gagctgcctg agcaagctga
     1381 agtacatggt ggttatcgat ccgctggtga ctgaaacctc taccttctgg cagaaccacg
     1441 gtgagtcgaa cgatgtcgat ccggcgtcta ttcagactga agtattccgt ctgccttcga
     1501 cctgctttgc tgaagaagat ggttctatcg ctaactccgg tcgctggttg cagtggcact
     1561 ggaaaggtca ggacgcgccg ggcgaagcgc gtaacga
//
LOCUS       DAMZET010000363         1588 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.364, whole genome shotgun sequence.
ACCESSION   DAMZET010000363 DAMZET010000000
VERSION     DAMZET010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.364"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(1..753)
                     /locus_tag="POB34_004920"
                     /pseudo
     CDS             complement(1..753)
                     /locus_tag="POB34_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            complement(784..1110)
                     /locus_tag="POB34_004921"
     CDS             complement(784..1110)
                     /locus_tag="POB34_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921729.1"
                     /translation="MKYIIFLFRAIWLALSLLILFFSMHRLSLLDSTRDVSELISLMS
                     YGMMVICFPTGIVFFIALIFIGTVSDIIGVRIDSKYIMAIIIWLYFLSGGYIQWFVLS
                     KRIINK"
     gene            complement(1110..1529)
                     /locus_tag="POB34_004922"
     CDS             complement(1110..1529)
                     /locus_tag="POB34_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921730.1"
                     /translation="MGGTGERSGPDIWGNPFYHQYLCVPDGKGDYTCGGQDQRGESKG
                     DGLWGPGKASNDTKEAAGRCDLVETDNSCVENCLKGKFKEVRPRYSVLPDIFTPINLG
                     LFKNCQDWSNDSLETCKMKCSGNNIGRLIRFVFTGVM"
BASE COUNT          386 a          421 c          352 g          429 t
ORIGIN      
        1 gtggattttt cgcgccggcg tgtacaccgg gtccatctgg ttttgcatct gctccaccgt
       61 caggccgcac gatgccagcc agcggcggct ttcctcactc acccggtcag ccaggatttc
      121 actttccagc cggtcgagca tctgcaccag caccggcggg aacaccacac tgccaccgtc
      181 ttcgccgccg gactgctgaa gggtatccgc caggctgcgg cgctgcgttt tcgcctgctc
      241 accggtggcg gtttcaaccc tgatgagtgg cgtgaagctc cccggctgat aaatcgtctg
      301 gatgcgggtt ctgtcgttct gtattgtggt caggcggtcg ccgtcccagc cgtaccaggt
      361 cacttgcggt ttccgtgaca gcgacatcca gcccgtcagg tcccgttcac gtcgccacac
      421 ccgttttgcc accctgcggc ccagcgggtc gtaaagatag cggctttcga ccagcggctc
      481 tgcatattgt gtccgcgtgt agtgcaccag ccggtgctga ctgtcgtaat ggtaccggtg
      541 ggtgcgctca tcatccgtgc ggataacccc ttccgggatg aggtcggttt tctccgtcag
      601 cctgccgtga cggtcatacc ggtaaagata gtgcgcgtca cgggcgatac ggttatccgg
      661 ccacatgctg agggcgctgt ccgggtgcag ctccgggtcg ggcaggcggt tacctgccgg
      721 gtctgtggca tacgggatat caatgcccag gttacatata ataataagag caattaactt
      781 aatttatttg tttataatgc gcttacttaa tacaaaccat tgaatatacc ctcctgacag
      841 aaaataaagc catattatta tcgccataat atacttacta tcaatcctta cgccaataat
      901 gtctgataca gtccctataa atattagagc aataaagaat actatgcctg ttggaaaaca
      961 tattaccatc attccataag acatgagact aattaactcg ctcacatcac gagtggagtc
     1021 taatagtgaa agtctatgca tggaaaagaa tagtattaaa agcgataaag ccagccatat
     1081 agctctgaat aaaaaaataa tatatttcat tacatcactc cggtgaatac aaatctaata
     1141 agacgtccaa tgttatttcc ggagcacttc atcttacatg tttctaaaga atcattagac
     1201 cagtcttggc agtttttaaa tagccctaaa tttataggtg tgaatatatc aggcaataca
     1261 gaataacgcg gccttacctc tttaaacttc ccttttaaac agttctccac acaactatta
     1321 tcggtttcaa cgaggtcaca acggccagca gcttcttttg tatcattact tgctttacct
     1381 ggcccccata gaccatctcc ttttgattct ccccgttggt cttggccacc acaagtatag
     1441 tcccctttac catctgggac acaaagatat tgatgataga acggattccc ccatatatct
     1501 ggaccgcttc tttcaccagt tccgcccatt gaatgcagtg gcttaatgca ttttagtgtt
     1561 tctaatccta atggatctat atttgaga
//
LOCUS       DAMZET010000364         1562 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.366, whole genome shotgun sequence.
ACCESSION   DAMZET010000364 DAMZET010000000
VERSION     DAMZET010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.366"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..696
                     /locus_tag="POB34_004923"
     CDS             <1..696
                     /locus_tag="POB34_004923"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HDI5921731.1"
                     /translation="AFLACPEESVDYAVMERTADAVVVPMDAGWSDVGSWSSLWEISA
                     HTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTKDAVLIADRNAVQDVK
                     KVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITVKPGEGLSVQMHHHRA
                     EHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKIPLDLIEVRSGSYLEE
                     DDVVRFADRYGRV"
     gene            889..>1562
                     /gene="cpsG"
                     /locus_tag="POB34_004924"
                     /gene_synonym="manB"
     CDS             889..>1562
                     /gene="cpsG"
                     /locus_tag="POB34_004924"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HDI5921732.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPE"
BASE COUNT          382 a          389 c          460 g          331 t
ORIGIN      
        1 gcgtttctgg cctgcccgga agagtcggtg gattacgcgg tcatggaacg tacggcagat
       61 gccgttgtgg tgccgatgga tgcgggctgg agcgatgtcg gttcctggtc ttcattatgg
      121 gagatcagcg cccacaccgc cgagggcaac gtttgccacg gtgatgtgat taatcacaaa
      181 actgaaaaca gctatgtgta cgccgaatct ggcctggtca ccaccgtcgg ggtgaaagat
      241 ctggtagtgg tgcagaccaa agatgcggtg ctgattgccg accgtaacgc ggtgcaggat
      301 gtgaaaaaag tggtcgagca gatcaaagcc gatggtcgcc atgagcatcg ggtgcatcgc
      361 gaagtgtatc gtccgtgggg caaatatgac tctatcgacg cgggcgaccg ctaccaggtg
      421 aaacgcatca ccgtgaaacc gggcgagggc ttgtcggtac agatgcacca tcaccgcgcg
      481 gaacactggg tagtggtcgc gggaacggca aaagtcacca ttgatggtga tatcaaactg
      541 cttggtgaaa acgagtccat ttatattccg ctgggggcga cgcactgcct ggaaaacccg
      601 gggaaaattc cgctcgattt aattgaagtg cgctccggct cttatctcga agaggatgat
      661 gtggtgcgct tcgcggatcg ctacggacgg gtgtaaacgt cgcatcagga gctgtttgtc
      721 ggatgcggcg tgaacgcctt atccgaccta cggttcgggt ttgtagtcct gataagacgc
      781 ggcagcgtcg catcaggcaa tgaatgcgaa accgagatgt aaataacgac aaaaacaaaa
      841 ttggccgttt cggtcagggc caactattgc ctgaaaaagg gtaacgacat gaaaaaatta
      901 acctgcttta aagcctatga tattcgcggg aaattaggcg aagaactgaa tgaagatatc
      961 gcctggcgca ttggtcgcgc ctatggcgaa tttctcaaac cgaaaaccat tgtgttaggc
     1021 ggtgatgtcc gtctcaccag cgaaacctta aaactggcgc tggcaaaagg tttacaggat
     1081 gcgggcgttg acgtgctgga tattggtatg tccggcaccg aagagatcta tttcgccacg
     1141 ttccatctcg gcgtggatgg cggcattgaa gttaccgcca gccataatcc gatggattac
     1201 aacggcatga agctggtgcg cgagggggct cgcccgatca gcggtgatac cggactgcgc
     1261 gatgtccagc gcctggctga agccaacgac tttcctcccg tcgatgaaac caaacgcggt
     1321 cgctatcagc aaatcaacct gcgtgacgct tacgttgatc acctgttcgg ttatatcaat
     1381 gtcaaaaatc tcacgccgct caagctggtg atcaactccg ggaatggcgc agcgggtccg
     1441 gtggtggacg ccattgaagc ccgctttaaa gccctcggcg cgcccgtgga attaatcaaa
     1501 gtacacaaca caccggacgg caatttcccc aacggtattc ctaacccgct gctaccggaa
     1561 tg
//
LOCUS       DAMZET010000365         1483 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.368, whole genome shotgun sequence.
ACCESSION   DAMZET010000365 DAMZET010000000
VERSION     DAMZET010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.368"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            200..562
                     /gene="queD"
                     /locus_tag="POB34_004925"
     CDS             200..562
                     /gene="queD"
                     /locus_tag="POB34_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDI5921733.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            640..>1483
                     /locus_tag="POB34_004926"
     CDS             640..>1483
                     /locus_tag="POB34_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDI5921734.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTVPLERCITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLH"
BASE COUNT          361 a          354 c          390 g          378 t
ORIGIN      
        1 aataaccaaa tcgactaacc tgttttagca atagtcttta acaacaaaat agattaacca
       61 acctaatgaa aaacaaatga atttagccaa tcattaagat aaatcagcga ttttgcgcaa
      121 caaagtcgtt ttagataatg cgaaaaaaca gcctttccgg tactctacgg cggttttatc
      181 gtcctgtaga gaaattatga tgtccaccac gttatttaaa gatttcacct tcgaagccgc
      241 tcaccgctta ccacacgtcc cggaagggca taaatgtggt cgcctgcacg ggcattcctt
      301 tatggtgcga ctggaaatta ccggggaagt cgatccgcat acgggctgga ttatcgattt
      361 cgctgaactg aaagcagcgt ttaagccaac ttatgagcgc ctcgatcacc attatctcaa
      421 tgatattcca ggcctggaaa acccgaccag cgaagtttta gcaaaatgga tttgggatca
      481 ggttaaaccc gttgtgccgc tgttaagtgc ggtgatggta aaagaaacct gcaccgctgg
      541 ttgtatctat cgcggcgaat gataagagtg tgtcggcggt caatttccct taagtaacgc
      601 tatgttaggg tgttgtgttc tggatatctg gggcatgaca tggaagacga ctgcgacatt
      661 attattattg gtgccggtat tgcaggcacc gcttgcgcgt tacgctgcgc gcgagcgggt
      721 ttatccgttt tgttactgga acgcgctgaa atccccggca gcaaaaatct ttccggcggg
      781 cggttatata cccatgcact cgcggaactc ctccctcagt ttcatctgac cgtgcctctt
      841 gaacgatgca tcactcacga aagcctttcc ctgttaacgc cggatggcgc aacgacgttt
      901 tccagcttac agcccggcgg tgaatcctgg agtgtattac gtgcacggtt cgatccgtgg
      961 ctggttgccg aagccgaaaa agaaggtgtc gaatgcatcc ctggtgcgac ggtggatgca
     1021 ctgtatgaag aaaacggcag ggtgtgtggt gtcatttgtg gtgacgatat tctccgcgcc
     1081 cgttatgtgg tgctggcaga aggtgccaac agcgtcctgg ctgagcgtca cgggttagtg
     1141 actcgtcctg ctggcgaagc gatggcgttg gggatcaaag aagtgctgtc gctggaaaca
     1201 tccgctattg aagaacgttt tcatctggag aataacgaag gcgcagcgtt gctgttcagc
     1261 ggcgggatct gtgatgactt acccggcggc gcatttcttt atactaatca acaaacgctc
     1321 tcgttaggga ttgtttgccc gctctcttcc cttacgcaaa gccgtgttcc ggcaagcgag
     1381 ctgctgactc gctttaaagc gcatccggca gtgcgcccgc ttatcaaaaa cacggaatca
     1441 ctggagtatg gtgcgcatct ggtgccagaa ggtggcttgc aca
//
LOCUS       DAMZET010000366         1451 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.369, whole genome shotgun sequence.
ACCESSION   DAMZET010000366 DAMZET010000000
VERSION     DAMZET010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.369"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..806
                     /gene="fusA"
                     /locus_tag="POB34_004927"
     CDS             <1..806
                     /gene="fusA"
                     /locus_tag="POB34_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDI5921735.1"
                     /translation="LAKEDPSFRVWTDEESNQTIIAGMGELHLDIIVDRMKREFNVEA
                     NVGKPQVAYRETIRQKVTDVEGKHAKQSGGRGQYGHVVIDMYPLEPGSNPKGYEFIND
                     IKGGVIPGEYIPAVDKGIQEQLKAGPLAGYPVVDMGIRLHFGSYHDVDSSELAFKLAA
                     SIAFKEGFKKAKPVLLEPIMKVEVETPEENTGDVIGDLSRRRGMLKGQESEVTGVKIH
                     AEVPLSEMFGYATQLRSLTKGRASYTMEFLKYDEAPSNVAQAVIEARGK"
     gene            877..>1451
                     /locus_tag="POB34_004928"
     CDS             877..>1451
                     /locus_tag="POB34_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDI5921736.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILEL"
BASE COUNT          381 a          357 c          384 g          329 t
ORIGIN      
        1 gtctggctaa agaagacccg tctttccgtg tatggactga cgaagaatct aaccagacca
       61 tcatcgcggg tatgggcgaa ctgcacctcg acatcatcgt tgaccgtatg aagcgtgaat
      121 tcaacgttga agcgaacgta ggtaaaccgc aggttgctta ccgtgaaact atccgccaga
      181 aagttaccga tgttgaaggt aaacacgcga aacagtctgg tggtcgtggt cagtatggtc
      241 atgttgttat cgacatgtac ccgctggagc cgggttcaaa cccgaaaggc tacgagttca
      301 tcaacgacat taaaggtggt gtaatccctg gcgaatacat cccggccgtt gataaaggta
      361 tccaggaaca gctgaaagca ggtccgctgg caggctaccc ggtagtagac atgggtattc
      421 gtctgcactt cggttcttac catgacgttg actcctctga actggcgttt aaactggctg
      481 cttctatcgc ctttaaagaa ggctttaaga aagcgaaacc agttctgctt gagccgatca
      541 tgaaggttga agtagaaact ccggaagaga acaccggtga cgttatcggt gacttgagcc
      601 gtcgtcgtgg tatgctcaaa ggtcaggaat ctgaagttac tggcgttaag atccacgctg
      661 aagtaccgct gtctgaaatg ttcggatacg caactcagct gcgttctctg accaaaggtc
      721 gtgcatcata cactatggaa ttcctgaagt atgatgaagc gccgagtaac gttgctcagg
      781 ccgtaattga agcccgtggt aaataagcct aagggttaat accaaagtcc cgtgctctct
      841 cctgaagggg agagcactat agtaaggaat atagccgtgt ctaaagaaaa atttgaacgt
      901 acaaaaccgc acgttaacgt tggtactatc ggccacgttg accacggtaa aactactctg
      961 accgctgcaa tcaccaccgt actggctaaa acctacggcg gtgctgctcg tgcattcgac
     1021 cagatcgata acgcgccgga agaaaaagct cgtggtatca ccatcaacac ttctcacgtt
     1081 gaatacgaca ccccgacccg tcactacgca cacgtagact gcccggggca cgccgactat
     1141 gttaaaaaca tgatcaccgg tgctgctcag atggacggcg cgatcctggt agttgctgcg
     1201 actgacggcc cgatgccgca gactcgtgag cacatcctgc tgggtcgtca ggtaggcgtt
     1261 ccgtacatca tcgtgttcct gaacaaatgc gacatggttg atgacgaaga gctgctggaa
     1321 ctggttgaaa tggaagttcg tgaacttctg tctcagtacg acttcccggg cgacgacact
     1381 ccgatcgttc gtggttctgc tctgaaagcg ctggaaggcg acgcagagtg ggaagcgaaa
     1441 atcctggaac t
//
LOCUS       DAMZET010000367         1425 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.370, whole genome shotgun sequence.
ACCESSION   DAMZET010000367 DAMZET010000000
VERSION     DAMZET010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.370"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1146
                     /locus_tag="POB34_004929"
     CDS             <1..1146
                     /locus_tag="POB34_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI5921737.1"
                     /translation="AELEQAKIAIEQARRVGDLARMSELQYGKIPELEKQLEAATQLE
                     GKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQELHHRVIGQNEAVD
                     AVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFDSDEAMVRIDMSE
                     FMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKAHPDVFNILLQVL
                     DDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKELVLGVVSHNFRPE
                     FINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDEALKLLSENGYDP
                     VYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAVQ"
BASE COUNT          356 a          323 c          411 g          335 t
ORIGIN      
        1 gcggaactgg aacaggcgaa aatcgctatt gaacaggctc gccgtgtggg ggacctggcg
       61 cggatgtctg aactgcaata cggcaaaatc ccggaactgg aaaagcaact ggaagccgca
      121 acgcagctcg aaggcaaaac tatgcgtctg ttgcgtaata aagtgaccga cgccgaaatt
      181 gctgaagtgc tggcgcgttg gacggggatt ccggtttctc gcatgatgga aagcgagcgc
      241 gaaaaactgc tgcgtatgga gcaagaactg caccatcgcg tcattggtca gaacgaagcg
      301 gttgatgcgg tatctaacgc tattcgccgt agccgtgcgg ggttggcgga tccaaatcgc
      361 ccgattggtt cattcctgtt cctcggccca acgggtgtgg ggaaaacaga gctttgtaag
      421 gcgctggcga actttatgtt tgatagcgac gaggcgatgg tccgtataga tatgtccgag
      481 tttatggaga aacactcggt gtctcgtttg gttggtgcgc ctccgggata tgtcggttat
      541 gaagaaggtg gctacctgac cgaagcggtg cgtcgtcgtc cgtattccgt catcctgctg
      601 gatgaagtgg aaaaagcgca tccggatgtc ttcaacattc tgttgcaggt actggatgat
      661 gggcgtctga ctgacgggca ggggagaacg gtcgacttcc gtaatacggt cgtcattatg
      721 acctctaacc tcggttccga tctgattcag gaacgcttcg gtgaactgga ttatgcgcac
      781 atgaaagagc tggtgctcgg tgtggttagc cataacttcc gtccggaatt cattaaccgt
      841 atcgatgaag tggtggtctt ccatccgctg ggtgaacagc acattgcgtc gattgcgcag
      901 attcagttga aacgtctgta taaacgtctg gaagaacgtg gttatgaaat ccacatttct
      961 gacgaggcgc tgaaactgct gagcgagaac ggttacgatc cggtctatgg tgcacgtcct
     1021 ctgaaacgtg caattcagca gcagatcgaa aacccgctgg cacagcaaat actgtctggt
     1081 gaattggttc cgggtaaagt gattcgcctg gaagttaatg aagaccggat tgtcgccgtc
     1141 cagtaaatga taaaacgagc ccttcggggc tcgtttttgt ctataagtta gacggaaaag
     1201 actatattta agatgttttg cctgaaaaat gagcgaacga taaagttttt atctttttcg
     1261 cttgtcaggc cggaataact ccctataatg cgccaccact gacacggaac aacggcaaac
     1321 acgccgccgg ggcagcgggg ttctcctgag aatctcaaca gagaaaagca aaaaaatgct
     1381 tgactctgta gcgggaaagc gtattatgca caccccgcgc cgctg
//
LOCUS       DAMZET010000368         1404 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.371, whole genome shotgun sequence.
ACCESSION   DAMZET010000368 DAMZET010000000
VERSION     DAMZET010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.371"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1331
                     /gene="pqiB"
                     /locus_tag="POB34_004930"
     CDS             <1..1331
                     /gene="pqiB"
                     /locus_tag="POB34_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HDI5921738.1"
                     /translation="WVVKPQIGRKGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSP
                     PLAPPDAKGIRVILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINA
                     PYDRLVTNNVRFWKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAP
                     KTAFVLYDDQKSIQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFA
                     PNMRQTFNDDYRIPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTG
                     ALYVDLDFYPNTPAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIE
                     QATSTLYESQRTMKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSA
                     AYNKMVADMQRLDQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
BASE COUNT          375 a          312 c          392 g          325 t
ORIGIN      
        1 tttgggtggt gaaaccgcag attggtcgca aagggattag cggcctggga acgctgctgt
       61 ctggagttta tatcgaactg cagccaggcg cgaaaggcag caaaatggat aaatacgatt
      121 tgctggactc gccaccgttg gccccgcctg atgcgaaagg tatccgtgtg attctcgata
      181 gcaaaaaagc cgggcagctc tcgccaggag atccggtgct gttccgtggc tatcgggtag
      241 gttcggttga aaccagcacc ttcgatacac aaaaacgcaa tatcagctat caactgttca
      301 tcaatgcacc ttatgaccga ctggtgacca acaatgttcg cttctggaaa gatagtggca
      361 ttgcggttga tctgacgtca gcagggatgc gtgtggagat gggctcattg acaacgctgc
      421 tgagtggcgg tgtcagcttt gatgtgccgg aaggtctgga tttagggcag ccagtggcac
      481 cgaaaacagc tttcgttttg tatgatgatc agaagagcat tcaggattcg ttgtacaccg
      541 atcacattga ttatctgatg ttctttaaag attcggtacg cggtctgcaa ccgggagctc
      601 cggtagagtt ccggggtatt cgcctgggta ccgtaagcaa agtgccattc tttgcgccga
      661 atatgcgtca gacatttaac gatgattacc gtattccggt actgattcgt atcgagccag
      721 agcggctgaa aatgcagctt ggcgaaaatg cggatgttgt tgagcacctt ggcgaattgt
      781 tgaaacgtgg tttacgcgga tcgctgaaaa ccggaaacct ggtcactggt gcactgtatg
      841 ttgatctcga tttctatcca aatacgcctg caataaccgg tattcgtgaa tttaatggtt
      901 atcagattat cccgaccgtt agcggcggcc tggcgcaaat ccagcaacga ctgatggaag
      961 cgttggataa gatcaacaaa ctgccattga atccgatgat tgaacaggca accagtacgc
     1021 tttatgaaag tcagcgcaca atgaaaaacc tgcaaacgac gctggatagc atgaacaaga
     1081 tcctcgctag ccagtcgatg cagcagttgc cgacggatat gcagtcaacg ttgcgtgaat
     1141 tgaatcgcag catgcagggc ttccagcctg gctccgcagc ctacaacaag atggtggcgg
     1201 atatgcagcg ccttgatcag gtgttgcgag aactgcaacc ggtgctgaaa acgctcaatg
     1261 agaagagtaa cgcgctggta tttgaagcga aggacaaaaa agatccagag ccgaagaggg
     1321 cgaaacaatg aaaaagtggc tagtgacgat tgcagcactg tggctggccg gatgcagctc
     1381 cggcgaaatt aataaaaact atta
//
LOCUS       DAMZET010000369         1385 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.372, whole genome shotgun sequence.
ACCESSION   DAMZET010000369 DAMZET010000000
VERSION     DAMZET010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.372"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..82)
                     /locus_tag="POB34_004931"
     CDS             complement(<1..82)
                     /locus_tag="POB34_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotein"
                     /protein_id="HDI5921739.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDEL"
     gene            298..>1385
                     /gene="argS"
                     /locus_tag="POB34_004932"
     CDS             298..>1385
                     /gene="argS"
                     /locus_tag="POB34_004932"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDI5921740.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLADHVQQALAS
                     DRLDVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVP"
BASE COUNT          359 a          351 c          357 g          318 t
ORIGIN      
        1 aaagttcatc taatgcgtgg gtaaatcgcg gtaaatccga tgcaatatcc tgcagctcat
       61 cgatagattg ccagttcgcc ataatcactc ttcgtctttc agtaaaagcg ttaatttacc
      121 ctgttgccct gtgtcaacca accgctgatt tcacgccgct tctgatgcaa tagtgaaaac
      181 ggcaatacgc cacgcgcacg ttgctgacga aaacagccat ttgcagtata ctcccgccct
      241 aatttcttta actggtgcgg gcaatttttg ctcgcttcat caatgtaagg tattccggtg
      301 aatattcagg ctcttctctc agaaaaagtc cgtcaggcca tgattgcggc aggcgcgcct
      361 gcggattgcg aaccgcaggt tcgtcagtca gcaaaagttc agttcggcga ctatcaggct
      421 aacggcatga tggcagttgc taaaaaactg ggtatggcac cgcgacaatt agcagagcag
      481 gtgctgactc atctggatct taacggtatc gccagcaaag ttgagatcgc cggtccaggc
      541 tttatcaaca ttttccttga tccggcattc ctggctgatc atgttcagca ggcgctggcg
      601 tccgatcgtc tcgatgttgc tacgccagaa aaacagacca ttgtggttga ctactctgcg
      661 ccaaacgtgg cgaaagagat gcacgtcggt cacctgcgct ctaccattat tggtgacgca
      721 gcagtgcgta ctctggagtt cctcggtcac aaagtgattc gcgcaaacca cgtcggcgac
      781 tggggcactc agttcggtat gctgattgca tggctggaaa agcagcagca ggaaaacgcc
      841 ggtgaaatgg agctggctga ccttgaaggt ttctaccgcg atgcgaaaaa gcattacgac
      901 gaagatgaag agtttgccga gcgtgcacgt aactacgtgg taaaactgca aagcggtgac
      961 gaatatttcc gcgagatgtg gcgcaaactg gtcgacatca ccatgacgca gaaccagatc
     1021 acctacgatc gtctcaacgt gacgctgacc cgtgatgacg tgatgggcga aagcctctac
     1081 aacccaatgc tgccaggaat tgtggcggat ctcaaagcca aaggtctggc agtagaaagc
     1141 gaaggggcga ccgtcgtatt ccttgatgag tttaaaaaca aggaaggcga accgatgggc
     1201 gtgatcattc agaaaaaaga tggcggctat ctctacacca ccactgatat cgcctgtgcg
     1261 aaatatcgtt atgaaacact gcatgccgat cgcgtgctgt attacatcga ctcccgtcag
     1321 catcaacacc tgatgcaggc atgggcgatc gtccgtaaag caggctatgt accggaatcc
     1381 gtacc
//
LOCUS       DAMZET010000370         1385 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.373, whole genome shotgun sequence.
ACCESSION   DAMZET010000370 DAMZET010000000
VERSION     DAMZET010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.373"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..96)
                     /gene="pspB"
                     /locus_tag="POB34_004933"
     CDS             complement(<1..96)
                     /gene="pspB"
                     /locus_tag="POB34_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="DNA-binding transcriptional regulator; acts
                     together with PspC to induce psp operon during infection
                     with phage, exposure to ethanol or osmotic shock; forms a
                     complex with PspA and C; PspC is required for PspAB
                     binding; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HDI5921741.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGR"
     gene            complement(150..818)
                     /gene="pspA"
                     /locus_tag="POB34_004934"
     CDS             complement(150..818)
                     /gene="pspA"
                     /locus_tag="POB34_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HDI5921742.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKDREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            985..>1385
                     /locus_tag="POB34_004935"
     CDS             985..>1385
                     /locus_tag="POB34_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDI5921743.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGG"
BASE COUNT          320 a          349 c          330 g          386 t
ORIGIN      
        1 gcgaccagaa cgattgctgt aatgtagcca taaccagatc ggtaaaacaa acagcacaaa
       61 aatggttaac ggaatagcca gaaatagcgc gctcataagt actccttaga cgatgagcgg
      121 cggcgcgttc ggacgccgcc tggatgttat tattgattgt cttgcttcat tttggctttt
      181 aattgtgcca gttgctcgct tattgcatca tcggctttca gttcggcaaa ctgatcgtcc
      241 agcgattttt gtttaccgaa gctgtggctt tctgcttccg cttccatctg gtcaatacga
      301 cgttcgaaag attcgaaacg agccattgct tcatccagtt tgccactgtc cagctgacga
      361 cgcacatcgc gcgacgagtt tgccgcctga tggcgtaaca tcaatgcctg ctggcgagcg
      421 cgtgtttcgc tcaatttgtt ttccagctcg ccgatctctt tcttcatgcg tgccagcgta
      481 tcgtccacca gcgtcacttc atgttccagt gacttaatca gatcggtcag tttctgtttt
      541 tcaattaacg cagcacgcgc caggtcttct ctatctttca gcagtgccag ttcggctttt
      601 tcctgccatt caacttcacg agccgacgct tgttcaatgc ggcgagtaag ctgtttcttt
      661 tccgccagcg cacgcgccga agtagaacgc acttcaacca gcgtgtcttc catttcctgg
      721 atcatcagac gcaccagttt ctgcggatct tccgctttct ctaacagagc gttgatgttg
      781 gcattcacga tgtcggcaaa gcgagaaaaa atacccataa tgttgtcctc ttgatttctg
      841 cattcaggat tgtcctgctg aactgttagt acaatttgcg tgccaatttt ttatcttttt
      901 gatttataaa gatctgattg aagaatcaac agcaacatgc caggatgaat tagcgaatta
      961 cactaacaag tggcgaattt catcatggca gaatacaaag ataatttact cggtgaggcg
     1021 aacagctttc tcgaagtgct ggaacaggtt tcgcatctcg caccgctgga caaaccggtg
     1081 ctcatcatcg gcgaacgcgg caccggtaaa gagctgatcg ccagccgcct gcattatctc
     1141 tcctcccgtt ggcaagggcc gtttatttcc cttaactgcg cggcgttaaa tgaaaatctg
     1201 ctggattccg aactgtttgg tcacgaagcg ggggcgttta ccggtgcgca aaaacgtcat
     1261 ccaggaagat ttgaacgtgc cgacggcggt acgctatttc ttgatgaact cgctacagca
     1321 ccgatgatgg tgcaggaaaa attattgcgc gtgattgagt acggtgaact ggagcgcgtt
     1381 ggcgg
//
LOCUS       DAMZET010000371         1345 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.374, whole genome shotgun sequence.
ACCESSION   DAMZET010000371 DAMZET010000000
VERSION     DAMZET010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.374"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>1345
                     /gene="hrpA"
                     /locus_tag="POB34_004936"
     CDS             <1..>1345
                     /gene="hrpA"
                     /locus_tag="POB34_004936"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HDI5921744.1"
                     /translation="IIEVSGRTYPVEVRYRPIVEEADDTERDQLQAIFDAVDELSQES
                     PGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNRVFQSHSGRRIVLA
                     TNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQASANQRKGRCGRVSE
                     GICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAAFPFVEAPDKRNIQ
                     DGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLEAQKHGCVREAMII
                     TSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLGEQQKALSSNAFRR
                     LCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHIALLTGLLSHIGMK
                     DADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGRIAARIDPEWVE"
BASE COUNT          300 a          365 c          380 g          300 t
ORIGIN      
        1 gattatcgaa gtctccggtc ggacctatcc ggtggaagtg cgctatcgac cgattgttga
       61 agaagccgac gacaccgagc gcgatcagtt gcaggcgatt tttgacgccg tagacgaact
      121 gagtcaggaa agccctggcg acattctgat ctttatgagt ggtgagcggg aaattcgcga
      181 taccgccgat gcgctgaaca agctaaatct gcgtcatacc gaaatcttgc cgctttatgc
      241 gcggctttcg aacagcgaac agaaccgcgt gttccagtcg cacagcggac ggcgcattgt
      301 gctggcgacc aacgtcgcgg aaacctcgct gaccgtaccg gggattaaat acgttatcga
      361 ccccggtaca gcacgtatca gccgctacag ctatcgcacc aaagtgcagc gtttgccgat
      421 tgaacccatt tcgcaggcgt ctgccaatca gcgtaaaggc cgctgtgggc gtgtgtccga
      481 agggatctgt attcgtctct attccgaaga cgatttcctt tcgcgcccgg agtttaccga
      541 tccggagatt ctgcgtacca acctggcctc ggttattttg cagatgaccg cgctggggct
      601 gggcgatatc gctgcgttcc cgtttgtcga agcaccggat aaacgcaata tccaggatgg
      661 cgtgcgtctg ctcgaagaac tgggcgcgat caccacggat gaacaggcca gcgcctataa
      721 actgacgccg ctcggtcgcc agctctcgca gttgcctgtc gatccacgtc tggcgcgtat
      781 ggtgctggaa gcgcaaaaac atggctgcgt gcgtgaggcg atgattatca cgtccgcgct
      841 ttccattcag gatccgcgcg agcgtccgat ggataaacag caggcatcgg acgaaaaaca
      901 tcgtcgcttc cacgacaaag agtctgactt cctcgcgttt gtgaatctgt ggaattatct
      961 tggcgagcag caaaaggcgc tttcttccaa cgccttccgt cgcctgtgtc gtaccgatta
     1021 tctcaactat ctgcgcgtgc gcgaatggca ggatatctac acccagttgc gccaggtggt
     1081 gaaagaactt ggcattccgg ttaacagcga accggcggag tatcgcgaaa ttcacattgc
     1141 gttgctgacg ggtttacttt cccatatcgg catgaaagat gccgataaac aagaatatac
     1201 cggcgcacgt aacgcgcgtt tctccatctt ccccggttct ggtttattca aaaaaccgcc
     1261 gaaatgggta atggtggcgg aactggtaga aaccagccgc ctgtgggggc gcattgctgc
     1321 gcgtatcgac ccggaatggg tggag
//
LOCUS       DAMZET010000372         1320 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.375, whole genome shotgun sequence.
ACCESSION   DAMZET010000372 DAMZET010000000
VERSION     DAMZET010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.375"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..947)
                     /gene="nrfB"
                     /locus_tag="POB34_004937"
     CDS             complement(<1..947)
                     /gene="nrfB"
                     /locus_tag="POB34_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HDI5921745.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREY"
     gene            complement(1216..>1320)
                     /locus_tag="POB34_004938"
     CDS             complement(1216..>1320)
                     /locus_tag="POB34_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HDI5921746.1"
                     /translation="TNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGFS"
BASE COUNT          340 a          319 c          335 g          326 t
ORIGIN      
        1 aaatattcgc gcacgcagat catgtttgac gtccgtgcgt gctgtaaaaa tttgcgctgc
       61 tcgcgttctt tggcttcgtc caccaccgga aaacggacaa aaatttccgt catacctttc
      121 tctttcaggc ggaaaccaat gtcgtaatct tcagtcagac tctgcacgtc gaaagcaata
      181 ccgtcaccgt cagctaacag agcggtcacg gcgcggcggc tgaaacaggt gccgacgcct
      241 gcgctgggca cttgtccggc gagggcttca cgcaccggaa catctttgcc atgcagctct
      301 gaaaactcat caatgtaagt catgctggtg aagtgcgtcc attcgcgttc gaacggatac
      361 accgggatct gaatcagatc tttacgctcg accagatagt tgaacagacg caattccatc
      421 ggtgaaatca catcttcggc gtcatgcaga ataaaaccag caaaagcgaa attggcgcta
      481 cgttcaaact gggtgatggc gtccagcacg ttgttcagac agtcggcttt gctggtggga
      541 ccaggacgcg cgcagactac cttatgcacg ttcggaaagc gagcgcacac ttcgtcaaca
      601 tcacgttgag tatcggggtc gttggggtag gtgccaacaa agatatgata gttttcgtag
      661 tcgagcgtgg tcgccgccag ctcggccata ttgccgatga cgcccgtttc attccacgcc
      721 ggaaccataa tcgctaacgg tttttcatct ggtttataca gttcgcggta actcattcgc
      781 gggtagcggc gataaacact caacttgcgt ttaatgcggc gtacccagta gacgacatca
      841 ataaaaaaat cgtccagccc gctgatgaac atgatgaccg ctaacgttat cgcgattact
      901 tttaagccgt agagccaggt tgcaaaaaca tcaagaagcc agtccacaca aaaaccttac
      961 attaacgctg gttatgttta gggtggcgta tattaaggtt ttttatgaat tgtgacagct
     1021 ttttaccatt aataggtatg actattgcgg cacgttattt ttacactggt tataaaagtt
     1081 gccgtttgcg tagaatgtag acaaaagcat ggtgtcaagg aggggaggtt aacgtcatag
     1141 tgaaaaggag cggtggcacc gagcaggtca taatgtagtg gaagtgcctg gaggaggtga
     1201 gcaggcaggg caagattacg aaaacccgct ccccgcaagg actgacgcga gatagtttct
     1261 gtccatggct gcttttcgca tcttacgtct taaccctgcc ttgaatacct tatcattcgt
//
LOCUS       DAMZET010000373         1311 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.376, whole genome shotgun sequence.
ACCESSION   DAMZET010000373 DAMZET010000000
VERSION     DAMZET010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.376"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>1311
                     /gene="cheA"
                     /locus_tag="POB34_004939"
     CDS             <1..>1311
                     /gene="cheA"
                     /locus_tag="POB34_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HDI5921747.1"
                     /translation="LEAKGETPSAVTRLSVVAKSEPQDEQNRSQSPRRIILSRLKAGE
                     VDLLEEELGHLTTLTDVVKGADSFSAILPGDIAEDDITAVLCFVIEADQITFETVEVS
                     PKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLINLVGELVITQSM
                     LAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFSRYPRLVRDLAG
                     KLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKRLAAGKNSVGNL
                     ILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEVAMLIFAPGFST
                     AEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLTLAILDGMSVRV
                     ADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVEL"
BASE COUNT          333 a          333 c          372 g          273 t
ORIGIN      
        1 attagaagcg aaaggcgaaa cgccatccgc agtgacccga ttaagtgtgg ttgccaaaag
       61 tgaaccgcaa gatgagcaga atcgcagtca gtcgccgcga cgaattatcc tttcgcgcct
      121 gaaggccggc gaagtcgacc tgctggaaga agagctggga catctgacaa cgttaactga
      181 tgtggtgaaa ggggcggatt ctttctcggc aatattaccg ggcgacattg ccgaagatga
      241 catcacagcg gtactttgtt ttgtgattga agccgatcag attacctttg aaacagtaga
      301 agtctcgcca aaaatatcca ccccaccagt gcttaaactg gcagccgaac aagcgccaac
      361 cggtcgcgtg gagcgggaaa aaacgacacg tagcagtgaa tccaccagca tccgtgtagc
      421 ggtagaaaag gttgatcaat taattaacct cgtcggcgag ctggttatca cccagtccat
      481 gcttgcccag cgttccagcg aactggaccc ggttaatcat ggtgatttga ttaccagcat
      541 ggggcagtta caacgtaacg ctcgtgattt gcaggaatca gtgatgtcga ttcgcatgat
      601 gccgatggaa tatgtcttta gccgttatcc gcggctggtg cgtgatctgg cgggaaaact
      661 cggcaagcag gtagaactga cgctggtggg cagttccact gaactcgaca agagcctgat
      721 tgaacgcatt atcgacccgc tgacccacct ggtacgcaat agcctcgatc acggtattga
      781 actgccagaa aaacggctcg ccgcaggtaa aaacagcgta ggaaatttaa ttctttctgc
      841 cgaacatcag ggcggcaaca tttgcattga agtgaccgac gatggggcgg ggctaaaccg
      901 tgaacgaatt ctggcaaaag cggcctcgca agggctgacc gtcagcgaaa acatgagcga
      961 cgacgaagtc gcgatgctga tatttgcacc aggcttctcc acggcagagc aggtcaccga
     1021 cgtctccggg cgcggcgtcg gcatggacgt cgttaaacgt aatatccagg agatgggcgg
     1081 tcatgtcgaa atccagtcga agcaggggac tggcactacg atccgcattt tactgccgct
     1141 gacgctggcc atcctcgacg gcatgtccgt acgcgttgcg gatgaagttt tcattctgcc
     1201 gctgaatgct gttatggaat cactgcaacc ccgtgaagcc gatctccatc cactggccgg
     1261 cggcgagcgg gtgctggaag tgcggggtga atatctgccc atcgtcgaac t
//
LOCUS       DAMZET010000374         1297 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.377, whole genome shotgun sequence.
ACCESSION   DAMZET010000374 DAMZET010000000
VERSION     DAMZET010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.377"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            474..>1297
                     /locus_tag="POB34_004940"
     CDS             474..>1297
                     /locus_tag="POB34_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDI5921748.1"
                     /translation="MISRENNKNNLFRVKSKNGVLRCLKAGMDINMCNSKGQTALFTC
                     NVPEAIQAMIDADIDIHHLDNDGNNALFYAQNVETVELLVSNGINVNHRNKSGTLAIQ
                     HIDVSPGLVKYLIKAGLDIHTKDSYGNSFLFIPFEGYVYDALIEAGCDINHKNISGQT
                     AFDHWQSENNSIVGKYTHWESKNDNYIRFLIRNIHLKDSSKIVFTNITFKSIELLSLL
                     IKQKNEFEISDKCIITPTNADAKKLILQLKKLTDISHVWFYISSTYLLTHYAGRLL"
BASE COUNT          483 a          235 c          193 g          386 t
ORIGIN      
        1 agcatcaccc cttcctggct gttgaaaaag actacgctgc ttaaatttta aatgagcatc
       61 ctcaagccga ataacttaaa ggatgctctt caaattttaa taagaattat caacagcaaa
      121 aaacctcata aaaaacacta ctcatttaat ttccaaaatt aagatgaaca ttaataacca
      181 ccattatatt acatgatctt ttccatatcc tatagcatta ctctctatag tattaatatc
      241 tgagcctcta attatctcct ttatacagat tagttccaat atggataaat attcttgtga
      301 aaatggttaa tccacatcac ataggataac cttaacgtcc ctatgttcca ttaagttaag
      361 aataacctta agcaaaccaa atcagatacc ccctaataaa acaaaaataa atttgaatac
      421 cgtaaaatct ggtgtcatta atatcttgaa aacaacaaaa aaggagataa acaatgatat
      481 caagagaaaa taataaaaat aacctattcc gtgtaaagtc taaaaatggt gtccttcgtt
      541 gccttaaggc tggcatggat ataaatatgt gcaatagtaa aggtcaaact gctcttttta
      601 cctgtaatgt acctgaagct atacaggcaa tgattgacgc tgacattgac attcaccact
      661 tggataatga cggtaataat gcattattct atgctcaaaa tgttgaaaca gtggaacttc
      721 ttgtcagcaa tggtattaat gttaaccacc ggaataagtc aggcaccctt gcgattcaac
      781 atattgatgt ttcaccaggg ttagtaaaat atcttatcaa agcaggacta gatatccata
      841 ccaaagatag ctatggcaat tcctttcttt tcattccatt tgaaggatat gtgtacgatg
      901 cactaattga ggctggttgc gacattaatc ataaaaatat aagtggacag acagcattcg
      961 atcactggca gtcagaaaat aatagcatag ttggtaaata tacgcactgg gaatctaaaa
     1021 acgataacta cattcgtttt cttatccgta atattcatct taaagacagt tcaaaaattg
     1081 tattcacgaa cattacattt aaatcaattg agctgttgtc gttacttata aagcaaaaaa
     1141 atgaatttga gataagtgat aagtgcatta tcacaccaac aaatgccgac gctaaaaaat
     1201 taattttaca actaaagaag ctgacagaca ttagtcatgt ttggttttac ataagcagta
     1261 catatctact gacgcactat gcaggaagac tgctaat
//
LOCUS       DAMZET010000375         1289 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.378, whole genome shotgun sequence.
ACCESSION   DAMZET010000375 DAMZET010000000
VERSION     DAMZET010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.378"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            71..>1289
                     /locus_tag="POB34_004941"
     CDS             join(71..395,395..>1289)
                     /locus_tag="POB34_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HDI5921749.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRAKKLDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDV
                     LLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFN
                     SETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRS
                     PESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQRACNGLSDNRCL"
BASE COUNT          326 a          307 c          380 g          276 t
ORIGIN      
        1 cagacaacca gtatcactta aataagtgat agtcttaata ctagttttta gactagtcat
       61 tggagaacag atgattgatg tcttagggcc ggagaaacgc agacggcgta ccacacagga
      121 aaagatcgca attgttcagc agagctttga accggggatg acggtctccc tcgttgcccg
      181 gcaacatggt gtagcagcca gccagttatt tctctggcgt aagcaatacc aggaaggaag
      241 tcttactgct gtcgccgccg gagaacaggt tgttcctgcc tctgaacttg ctgccgccat
      301 gaagcagatt aaagaactcc agcgcctgct cggcaagaaa acgatggaaa atgaactcct
      361 caaagaagcc gttgaatatg gacgggcaaa aaagtggata gcgcacgcgc ccttattgcc
      421 cggggatggg gagtaagctt agtcagccgt tgtctccggg tgtcgcgtgc gcagttgcac
      481 gtcattctca gacgaaccga tgactggatg gatggccgcc gcagtcgtca cactgatgat
      541 acggatgtgc ttctccgtat acaccatgtt atcggagagc tgcccacgta tggttatcgt
      601 cgggtatggg cgctgcttcg cagacaggca gaacttgatg gtatgcctgc gatcaatgcc
      661 aaacgtgttt accggatcat gcgccagaat gcgctgttgc ttgagcgaaa acctgctgta
      721 ccgccatcga aacgggcaca tacaggcaga gtggccgtga aagaaagcaa tcagcgatgg
      781 tgctctgacg ggttcgagtt ctgctgtgat aacggagaga gactgcgtgt cacgttcgcg
      841 ctggactgct gtgatcgtga ggcactgcac tgggcggtca ctaccggcgg cttcaacagt
      901 gaaacagtac aggacgtcat gctgggagcg gtggaacgcc gcttcggcaa cgatcttccg
      961 tcgtctccag tggagtggct gacggataat ggttcatgct accgggctaa tgaaacacgc
     1021 cagttcgccc ggatgttggg acttgaaccg aagaacacgg cggtgcggag tccggagagt
     1081 aacggaatag cagagagctt cgtgaaaacg ataaagcgtg actacatcag tatcatgccc
     1141 aaaccagacg ggttaacggc agcaaagaac cttgcagagg cgttcgagca ttataacgaa
     1201 tggcatccgc atagtgcgct gggttatcgc tcgccacggg aatatctgcg gcagcgggct
     1261 tgtaatgggt taagtgataa cagatgtct
//
LOCUS       DAMZET010000376         1280 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.379, whole genome shotgun sequence.
ACCESSION   DAMZET010000376 DAMZET010000000
VERSION     DAMZET010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.379"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..391)
                     /locus_tag="POB34_004942"
     CDS             complement(<1..391)
                     /locus_tag="POB34_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
                     /protein_id="HDI5921750.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNY"
     gene            complement(536..1039)
                     /gene="cheW"
                     /locus_tag="POB34_004943"
     CDS             complement(536..1039)
                     /gene="cheW"
                     /locus_tag="POB34_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDI5921751.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(1060..>1280)
                     /locus_tag="POB34_004944"
     CDS             complement(1060..>1280)
                     /locus_tag="POB34_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDI5921752.1"
                     /translation="VILQSGGRRYALLVDQLISQHQVVVKNLESNYRKVPGISAATIL
                     GDGSVALIVDVSALQAINREQRMANTAA"
BASE COUNT          327 a          335 c          289 g          329 t
ORIGIN      
        1 aatagttttt atatttttca tcaatattac gactggtagc gaccatttca ggtaacggtg
       61 ccatgctttt gaatttttta taatgcgtcg ctgcctgcgc caatgttttc ctggcgctat
      121 cgagcaattc aactttggcg ttactttgtt gattggagga atccatcatc atccgcaccg
      181 ctgaacgact caggttaatg cgcgtttgca gcattaaatc ccaggttgac gtcagctcgc
      241 cctgctgttc ccgcaattga ttggaaacca caaagctctt ctggctatgg tgaagggaag
      301 aaaaaaacag actgccggaa ataagctgta acagtgcgaa tacccccagc accattacca
      361 acagcgtgac tacgcggata cggttaatca taaggcacct tcctgaaaac aagttgatct
      421 cgttatcggc aagaaggggg gaaactttat tgctgatgcc acccgccgcg aaattgaaat
      481 aaaaaacccg atgcgcagat catcgggttc atttcaattg aggaaatcgg gagaattacg
      541 ccacttctga cgccgcgcta tctaacagcg ccatctcttc gctgttcagc agtttttcga
      601 tgttcaccag aatcaacata cggtcgccca gtgcgcccag tccagtgaga tattctgttg
      661 aaagcgtcac ggcaaactct ggtgccggac gaatttgctc cgccgtcaat gaaagcacgt
      721 ctgaaacgcc gtcaaccacg atgccgacca cccgctgtcc gagattcagg acgataacta
      781 ccgtgttgtc gttatagtcc acatccacct ggctgaactt aattcgtaag tcaacaatcg
      841 gcacaataac gccgcgcaga ttcgtgacgc ctttgataaa cgctggcgtg ttcgcaatcc
      901 gtgttacctg atcgtagcca cggatctcct gcactttcag gatatcaata ccgtactctt
      961 catcaccaag ggtaaatacc agaaattcct ggcctgacgg ctcgctggcc agctttgtta
     1021 cattcgtcat accggtcata ttgttacctt tttactcatt caggcggcgg tgttcgccat
     1081 acgttgttcg cggtttatcg cctgcaaggc ggagacatca acaatcagtg ccacgctgcc
     1141 gtcgccaaga atggtcgcag cagaaatgcc ggggactttg cgatagttac tttcaaggtt
     1201 tttaaccaca acctggtgtt gactaattaa ttgatccacc agcaaggcgt agcggcgacc
     1261 gccactttgt aagatcacaa
//
LOCUS       DAMZET010000377         1270 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.380, whole genome shotgun sequence.
ACCESSION   DAMZET010000377 DAMZET010000000
VERSION     DAMZET010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.380"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            23..154
                     /locus_tag="POB34_004945"
     ncRNA           23..154
                     /ncRNA_class="other"
                     /locus_tag="POB34_004945"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(338..1015)
                     /gene="narI"
                     /locus_tag="POB34_004946"
     CDS             complement(338..1015)
                     /gene="narI"
                     /locus_tag="POB34_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDI5921753.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(1015..>1270)
                     /locus_tag="POB34_004947"
     CDS             complement(1015..>1270)
                     /locus_tag="POB34_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="delta subunit of nitrate reductase 1; chaperone for
                     the insertion of the molybdenum cofactor and assembly of
                     nitrate reductase 1; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDI5921754.1"
                     /translation="RYAVLFDLLLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEE
                     EQVKFFADKGCGDSAITAHQRRFAGAVAPQYLNITTGGQH"
BASE COUNT          331 a          382 c          318 g          239 t
ORIGIN      
        1 tcaagcgaat ccgcaattaa atattctgcc catgcgggga aggatgagaa gcttcgacta
       61 aggttcgact tgagcgccag cgagagagcg ttgccgcagg caacgacccg aagggcgaag
      121 cgcgcagcgc tgagtaatcc ttcccccacc accatcgctt ccttaaataa tcaaaacctg
      181 ttactccgag ttaattttgc ccgatggcac cacgtttacc gggcctacaa tatcctcccc
      241 tgtaggtcgg ataagcgaag cgcatccgac aactcacccc gcaccgtgcc cataaaaaaa
      301 ccccgccgaa gcagggtcta tgtgaactaa aattcgctta gtgacgagcg cgcaccagct
      361 ggtacttacg tgtcagatac tccaccggta cgctccagat gtgtaccaga cgcgagaacg
      421 ggaacagcag gaataacgtc atccccagca ccaggtgcag acggaagata aacgccacac
      481 catcgaggtg ttgagaagcg ccaccgtgga aggtcaccac cgactgcgcc cagccaacca
      541 gtttcatcat ctcgctaccg tccatatgct gagcggagaa cggaatggtc aacaggccca
      601 gcgcgcactg gataacgagc agcgacagga tcaggatatc cgctccggta gtggttgcac
      661 gcacgcgtgg gctgaacaga cgacgcttca gcaacagcac gccgccaatc agacacagca
      721 cgccgctggc accaccagca aacatcgcca ttttctgttt cacttcaatc ggcagccagg
      781 cttcatacat ccagtgcggc gtcagcatac cgaagaagtg accaacaaaa atccccagaa
      841 taccgatatg gaacaggttc gacgccaggt tcatcccttt gcgatccagc atctggctgg
      901 acgccgcgcg ccaggtgtac tgcccgtagt cataacgcag ccagctacca atcaggaaga
      961 ccgccccggc tatgtacggg tagatatcaa agaagaacat attcaggaat tgcattagtg
     1021 ctgtcctccg gtggtgatat tcagatattg cggcgcgacg gcaccggcaa agcgacgctg
     1081 atgagcagtg attgctgaat caccgcagcc tttgtcagca aagaatttaa cctgctcttc
     1141 ttcccagaca gcatccagcg cctgcggcgt atcatcgcgc gcttcgtcgg caattttttc
     1201 cgccactttg tcgctgtcga tagcggtatt cgccagtttc agcagcagat caaacagcac
     1261 ggcataacgg
//
LOCUS       DAMZET010000378         1269 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.381, whole genome shotgun sequence.
ACCESSION   DAMZET010000378 DAMZET010000000
VERSION     DAMZET010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.381"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(602..808)
                     /locus_tag="POB34_004948"
     CDS             complement(602..808)
                     /locus_tag="POB34_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921755.1"
                     /translation="MNKEQSADELSLDLIRVKNMLNSTISMSYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNSKDYSLVVD"
     gene            1109..>1269
                     /locus_tag="POB34_004949"
     CDS             1109..>1269
                     /locus_tag="POB34_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HDI5921756.1"
                     /translation="MAQAAIFKEIFDQVRKNLNCELFYSELKRHNISLYIYYLATDNI
                     HIVLENDNIV"
BASE COUNT          417 a          275 c          233 g          344 t
ORIGIN      
        1 agtaataagc atcaccgatc ccgctcacat ttgacataag caactccatc tggcaggtga
       61 aaatcatgtt tatttatata tttcaattaa ttgtaaactg gtctaatgac agggagaaaa
      121 aaatattgta caggtaaaaa gagaaataac ttttaattat caataaatta ccaaacatgc
      181 tgccgccgcc atggaaatgc aaaaactagc ctttttccgc gacgctcccg ccccgtggca
      241 ggggccccca ccgggaggac ccgacagcct gacagccgtg acgagcatct gatacagcgc
      301 tttacataat ggcataggaa taattcagaa ggacatcaca gcatgcccac acaaattagt
      361 gtgagtgtcc tgtttcttcc tacgcacagg actgacgagc atgaggggaa aatatgcgaa
      421 ccataaatgc ctcatcttca gcaatgcagc cggcatccga acaggacgat attgtaaatt
      481 cacctaatta cgaggacatt gcagtagttg aattgcagct ctgtattagc ctgacagtga
      541 cagaatgcga tattgactct gtcacaggtg aaatagtttg aatgattagc agttatggtg
      601 ctcagtcaac caccagggaa taatccttcg aattcttatc gtgcttcacc aacgctgcct
      661 caattgccct gaatgcttcc agagacacct gatgttctat acatgcaatt acaacatccg
      721 ggtaactcat agaaatggtg ctattaagca tattttttac acgaatcaga tccaacgaga
      781 gttcatcagc agattgttct ttattcattt tgtcgctcca tgcgtttgct cgtcatctag
      841 cggttaaaat attacttcaa atcttactgc ataaaagagg aaaagtatga aagtttgagt
      901 acatcgacct tacatacatc tgtcggttgc atatccctcc tggatgccag caaggctcaa
      961 ttttgttacg caatcaacac tattcattaa aaacaggctt aatatttgac ataaatcatc
     1021 aacaaaacac aaagaggtca gaccagattg aaacaataaa cacgataatg aaaactacgc
     1081 gccatcgtat cacatggaag gtttaccaat ggctcaggct gccattttta aagaaatatt
     1141 cgatcaagtg cgtaaaaatt taaactgtga attattttat tctgagctaa aacgtcacaa
     1201 tatctcactt tatatttact atttagccac agataatatt cacattgtgt tagaaaacga
     1261 caacatagt
//
LOCUS       DAMZET010000379         1262 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.382, whole genome shotgun sequence.
ACCESSION   DAMZET010000379 DAMZET010000000
VERSION     DAMZET010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.382"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..108
                     /locus_tag="POB34_004950"
     CDS             <1..108
                     /locus_tag="POB34_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDI5921757.1"
                     /translation="LSELVCACHARKGNVSCPLIASLQGEAGLARSAMP"
     gene            complement(345..1193)
                     /locus_tag="POB34_004951"
     CDS             complement(345..1193)
                     /locus_tag="POB34_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDI5921758.1"
                     /translation="MNNDELATRRAQAIAEDRCFSKGRLRDEFRMKPAPGAEPVKWYK
                     NTYGGRFAVYRIADCVPMREKRPLTSKQQLAGQRLSVLSRLNSTSGRMARQAYDWLSL
                     APLFLDTETTGLDNTAEALEIGLTDAAGQVVFETRLKPTVAIGAQAAAVHGISEQALC
                     GAPSWTDVARQLRHAIGDRPVIIFNARFDIRILKQTAAAHSDPADWLEELTVYCAMEL
                     AAGYYGATNRYGTISLACAASQAGLTWEGQAHSAIADARMTAGVVNAIAAYHLELLQE
                     QARLKI"
BASE COUNT          261 a          372 c          314 g          315 t
ORIGIN      
        1 ctgtctgaac tcgtgtgcgc ctgccatgca cgaaagggga atgtttcctg cccgttgatc
       61 gcgtcactac agggcgaagc aggcctggca aggtcagcta tgccttagcg tgctttattt
      121 tccgttttct gaggtgcccc cagccagata atccttttca agtttttcag ccgtgtagca
      181 cctgccgcct aaaaaaagcc gcatggcctc cctggttttg tctctcacat atcatccctc
      241 attaatgcta atgtacacat acattagcat taacatgctg acagcaacaa gcggcggatt
      301 tacgtctgga taacgtaaat gattgcggta tacaggccag gcggtcagat tttcagccgt
      361 gcctgttcct gcagcagttc cagatgatat gcagcaatag cgtttaccac ccctgccgtc
      421 atccgtgcgt cggcgatcgc tgagtgtgcc tgcccttccc aggtcagtcc ggcctggctg
      481 gcagcacagg ccagtgaaat cgtgccatag cggttggtgg ccccataata tcctgcagcc
      541 agctccatcg cacaatatac cgtcagttct tccagccagt cagccggatc gctatgtgcg
      601 gcagcagtct gtttcagaat gcggatgtcg aaccgggcat taaagataat tactggtcgg
      661 tccccgattg catgacgcag ttgccgcgcc acatctgtcc atgatggtgc gccgcagaga
      721 gcctgctcgc tgataccatg cacagcggcg gcctgcgccc cgatggcgac cgtgggcttc
      781 agcctggttt caaataccac ctgaccggcc gcatcggtca ggccgatttc cagggcttcc
      841 gcggtattat ccagtcctgt cgtttcagta tccagaaaga gcggtgccag actcagccag
      901 tcatatgcct gccgtgccat ccggccactg gtgctgttaa gtctggataa aacggaaagt
      961 cgctggccag ccagctgttg tttactcgtc agagggcgtt tctcacgcat cggaacacag
     1021 tctgcaattc ggtatacagc gaacctgcca ccataagtgt ttttatacca cttaaccggc
     1081 tcagcaccgg gggctggttt cattctgaat tcatcgcgca aacgtccttt cgagaaacac
     1141 ctgtcttcgg caatcgcctg cgcacgtctg gtggccagct catcattatt catggttatt
     1201 gcatctcctt tccttatatc cggacagccc ctctatggcc acttctgtgc agtatgcaac
     1261 ac
//
LOCUS       DAMZET010000380         1235 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.383, whole genome shotgun sequence.
ACCESSION   DAMZET010000380 DAMZET010000000
VERSION     DAMZET010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.383"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(24..599)
                     /locus_tag="POB34_004952"
     CDS             complement(24..599)
                     /locus_tag="POB34_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HDI5921759.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(616..876)
                     /locus_tag="POB34_004953"
     CDS             complement(616..876)
                     /locus_tag="POB34_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDI5921760.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQTAERLISACPAGLFSLTPE
                     GDLRIDYRSCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(867..>1235)
                     /locus_tag="POB34_004954"
     CDS             complement(867..>1235)
                     /locus_tag="POB34_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDI5921761.1"
                     /translation="CVNTGISVRGMDMALTGAQAAAQTLISACQHREPQNLFPLYHHN
                     VERSLLWDVLQRYQHVPALLQRPGWYRTWPALMQDISRDLWDQGDKPVPPLRQLFWHH
                     LRRHGLWHLAGDVIRSLRCL"
BASE COUNT          324 a          330 c          299 g          282 t
ORIGIN      
        1 ttcaattggt tacgattatc ccgtcaaagt aattttttcg ctaacgtcca gaccccggta
       61 ttcgtggtgg aaagcgccac gacacccgcg ttaatcgcat tacgcgcatc ttcttcatcg
      121 cacaccagcc caccggcaat cagcggttgg cggattttct ctgtcaccca gcccagcact
      181 ttgggcatac agcctggcag gatctcaata caatccggat tcgattgcgc aacttgctta
      241 tcaatgttgt gaaacgaaat tgaatcaaca ataaacaggc gatgaataca gaaaaaacct
      301 tccgctcttg ccgctttcaa cattgaggct ttggtactga taatgccgtc cgcctcggtc
      361 accagtttca aaaactgaat aacgacttct ttatttgacg cgccttccag caaatcaaca
      421 tgaataaaag catatttccc ggcgttctta atctttttaa cgatattact gatggtgcag
      481 atattgccgt acaacacgga aataaattgg cattcagaat cgattgccag ttgcaggctg
      541 gcattatctt taacggcagc aatcaccgga ttctggcgga gcaggtgtaa gaggggcata
      601 aattatcctt attatttatc caaagcggta ggtgatgccg aatccggaag gcggatagcg
      661 ccactgttgt agtgttgatt cgtcgcacag caaacggcag gtgccacact ccaggcaact
      721 gcgatagtca atacgtaagt caccttccgg tgtgagcgaa aaaagacctg ccggacaggc
      781 gctaatcaac cgttctgccg tctggcgctc aacggagtca gccggaacaa tgtgcggcgc
      841 atcagcaacg cgccagagat tacgggctac agacatcgca gactcctgat aacatcgccc
      901 gccagatgcc acaggccgtg acgacgtaaa tgatgccaga ataactggcg cagcggtgga
      961 acaggtttat caccctgatc ccataaatcg cgggaaatat cctgcattaa cgcaggccac
     1021 gtacggtacc atcccgggcg ttgcaaaagc gccggaacat gctgataacg ctgtagaaca
     1081 tcccacagca ggctgcgctc tacgttgtga tgataaagcg gaaacagatt ttgcggctcg
     1141 cggtgctggc aggcgcttat cagcgtttgt gccgccgcct gcgcgccagt cagcgccata
     1201 tccatgccgc gcacggaaat tccggtattg acgca
//
LOCUS       DAMZET010000381         1230 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.384, whole genome shotgun sequence.
ACCESSION   DAMZET010000381 DAMZET010000000
VERSION     DAMZET010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.384"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..967
                     /gene="wzxC"
                     /locus_tag="POB34_004955"
     CDS             <1..967
                     /gene="wzxC"
                     /locus_tag="POB34_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HDI5921762.1"
                     /translation="AILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLVSVAPNLRFGAW
                     LTADSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFA
                     KIQDDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLC
                     IVGLLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLL
                     VQIINTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYVLGIVLKGQLALGM
                     LLAVQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
BASE COUNT          227 a          306 c          373 g          324 t
ORIGIN      
        1 cgcgatcctc ggttatctgg tcaatagtgc ggtaagaacg ctgctgtttg gctactttgg
       61 ccgcaaaatt tatcgccccg gtctgcactt ctcgctggtg tcggtggcac cgaacttacg
      121 ctttggtgcc tggctgacgg cggacagcat catcaactat ctcaacacca acctttcaac
      181 gctggtgctg gcgcgtattc tcggcgcggg cgtggccggg ggatacaacc tggcgtacaa
      241 cgtggccgtt gtgccgccga tgaagctgaa cccgatcatc acccgtgtgt tgtttccggc
      301 attcgccaaa attcaggacg acaccgaaaa gctgcgcgtc aacttctaca agctgctgtc
      361 ggtggtgggg attattaact ttccagcgct gctggggctg atggtggtgt cgaataactt
      421 cgtaccgctg gtattcggcg aaaagtggaa cagcattatt ccggtgctgc aattgctgtg
      481 tattgtgggt ctgctgcgct ccgtgggtaa cccgattggt tcgctgctga tggcgaaagc
      541 gcgggtcgac atcagcttta aattcaacgt attcaaaaca tttctgttta ttccggcgat
      601 tgttattggt gggcagatgg cgggcgcaat cggcgtcacg ctcggtttcc tgctggtgca
      661 gattatcaac accattctga gttacttcgt gatgattaaa ccggttcttg gttccagtta
      721 tcgccagtac atcctgagtt tgtggctgcc gttttatctc tcgctgccga cgctggtggt
      781 cagttatgtg ctgggcattg tgctgaaagg gcaactggcg ctggggatgc tgctggcggt
      841 acaaatagcc gcgggcgtgc tggcgtttgt ggtgatgatt gtgctgtcgc gccatccgct
      901 ggtggtggaa gtgaagcgtc agttttgtcg cagcgaaaaa atgaaaatgc ttttacgggc
      961 ggggtgagtt gttgttttcg gttccgacaa cggttgccgg gtgcagcgta aaacgcctta
     1021 tccatcctac ggttctgtgc tcgggacctg taggcctgat aagacgcgtc agcgtcgcat
     1081 caggcactga atgccggatg cggcgtgaac gccttatccg gcctacggtt cagggcttgg
     1141 atcttgtagg cccgataaga cgcgccagcg tcgcatcagg cactgaatgc cggatgcggc
     1201 gtaaaacgcc ttatccggcc tacggttctg
//
LOCUS       DAMZET010000382         1209 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.385, whole genome shotgun sequence.
ACCESSION   DAMZET010000382 DAMZET010000000
VERSION     DAMZET010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.385"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(273..>1209)
                     /locus_tag="POB34_004956"
     CDS             complement(273..>1209)
                     /locus_tag="POB34_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921763.1"
                     /translation="SLRMDFFGYNALIIQRSDNTCELTINEPLKNQEISTGNINVNFP
                     LKDIYNEIRRLNVVFSCGTGGIVDLSSLDLRNIDLELYDFTDKHMANAILNPFKLDDT
                     DFTNANMFQVNFVSSKQNTTISWDYLLKITPVLTSISDMYSEEKIKLVESCLNELGDI
                     TEEQLKIMRFAIIESIPRATLTDQLENELTKEIYKNSSKINNYLNRIKLPEMKGFSSE
                     KIDYYIDIIIKDYESVKENAYLIDPKINYNTDLNIEDSSSEEFLSDNTLEKDENSPDN
                     CFEVVKYNTYEAYNSENLYFTREEYTYDYDLLNAI"
BASE COUNT          352 a          193 c          211 g          453 t
ORIGIN      
        1 tgtacgtaat aaaccgttcc ggcgggtttc agattgttga gtgcgcttta ttcatgccgg
       61 atgcggcgtg aacgccttat cctgcctaca aaagcacgca aattcaatat attgcagaga
      121 tggcgtaggc ctgataagcg tagcgcatca ggcatttttg cggttgccat cagtttcaaa
      181 cccgcgtcag tgagtttttg cttttggtgc acagggtaag atgaatgaat atctacggta
      241 ttaattatag attttgtaag aagttattaa gtctatattg catttaaaag gtcgtaatca
      301 tacgtatatt cctctctggt aaaatataga ttctctgagt tatatgcttc atatgtgtta
      361 tattttacaa cctcaaagca attgtcagga gaattttcgt ctttctcgag tgtattatct
      421 gaaaggaatt cctctgagct agaatcctct atgtttaaat ctgtattgta attaattttt
      481 ggatcgataa gataagcatt ttctttgacg ctttcataat ctttaatgat tatatcgatg
      541 taataatcta ttttttctga tgagaatcct ttcatctctg gtaatttaat tctgttcaag
      601 taattgttaa ttttcgatga gtttttatat atttcttttg ttaattcatt ttctaattga
      661 tctgttagag ttgctcttgg tatagattct ataattgcaa atctcatgat ttttaattgt
      721 tcttcggtaa tatctccaag ctcatttaaa caactttcga caagcttaat tttttcttca
      781 gaatacatat cgctaatgct tgttagaaca ggcgttattt ttagtaaata atcccaggag
      841 attgtggtgt tttgttttga actaacgaaa tttacctgaa acatgttggc attagtaaaa
      901 tctgtgtcat ccaatttaaa aggatttaaa attgcattgg ccatatgttt atctgtaaaa
      961 tcataaagtt ctaaatcgat attccgtaag tccagagagg atagatcaac tatacctcca
     1021 gtcccacaac taaaaactac gttcaacctc ctgatttcat tgtaaatgtc tttcaatggg
     1081 aaattaacat tgatattgcc tgtggatatt tcctggtttt ttaacggttc attaattgta
     1141 agttcacagg tattgtctga tcgttgaata attagggcgt tataaccaaa aaaatccata
     1201 cgaagagac
//
LOCUS       DAMZET010000383         1174 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.386, whole genome shotgun sequence.
ACCESSION   DAMZET010000383 DAMZET010000000
VERSION     DAMZET010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.386"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..109
                     /locus_tag="POB34_004957"
     CDS             <1..109
                     /locus_tag="POB34_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDI5921764.1"
                     /translation="RLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            102..1097
                     /gene="fliG"
                     /locus_tag="POB34_004958"
     CDS             102..1097
                     /gene="fliG"
                     /locus_tag="POB34_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDI5921765.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            1090..>1174
                     /gene="fliH"
                     /locus_tag="POB34_004959"
     CDS             1090..>1174
                     /gene="fliH"
                     /locus_tag="POB34_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310706.2"
                     /note="binds to and inhibits the function of flagella
                     specific ATPase FliI; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDI5921766.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEP"
BASE COUNT          279 a          311 c          348 g          236 t
ORIGIN      
        1 acgtctgggg gcagaagtca tgagccagcg tatccgtgaa atgtctgata acgatccgcg
       61 cgtggtggcg ctggtcattc gccagtggat aaataacgat catgagtaac ctgacaggca
      121 ccgataaaag cgtcatcctg ctgatgacca ttggcgaaga ccgggcggca gaggtgttca
      181 agcacctctc ccagcgcgaa gtgcaaaccc tgagcgctgc aatggcgaac gtcacgcaga
      241 tctccaacaa gcagctaacc gatgtgctgg cggagtttga gcaagaagct gaacagtttg
      301 ccgcactgaa tatcaacgcc aacgattatc tgcgttcggt attggtcaaa gctctgggtg
      361 aggaacgtgc cgccagcctg ctggaagata ttctcgaaac tcgcgatacc gccagcggta
      421 ttgaaacgct caactttatg gagccgcaga gtgccgccga tctgattcgc gatgagcatc
      481 cgcaaattat cgccaccatt ctggtgcatc tgaagcgcgc ccaggccgcc gatattctgg
      541 cgttgttcga tgaacgtctg cgccacgacg tgatgttgcg tatcgccacc tttggcggcg
      601 tgcagccagc cgcgctggcg gaactgaccg aagtactgaa tggcttgctc gacggtcaga
      661 atctcaagcg cagcaaaatg ggcggcgtga gaacggcagc cgaaattatc aacctgatga
      721 aaactcagca ggaagaagcc gttattaccg ccgtgcgcga attcgacggc gagctggcgc
      781 aaaaaattat cgacgagatg ttcctgttcg aaaatctggt ggatgtcgac gatcgcagca
      841 ttcagcgtct gttgcaggaa gtggattccg aatcgctgtt gatcgcgctg aaaggggccg
      901 agcagccact gcgcgagaaa ttcctgcgca atatgtcgca gcgtgccgcc gatattctgc
      961 gcgacgatct cgccaaccgt ggtccggtgc gtctgtcgca ggtggaaaac gaacagaaag
     1021 cgattctgct gattgtgcgc cgccttgccg aaactggcga gatggtaatt ggcagcggcg
     1081 aggataccta tgtctgataa tctgccgtgg aaaacctgga cgccggacga tctcgcgcca
     1141 ccacaggcag agtttgtgcc catggtcgag ccgg
//
LOCUS       DAMZET010000384         1173 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.387, whole genome shotgun sequence.
ACCESSION   DAMZET010000384 DAMZET010000000
VERSION     DAMZET010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.387"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..361
                     /locus_tag="POB34_004960"
     CDS             <1..361
                     /locus_tag="POB34_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDI5921767.1"
                     /translation="SLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVADWRQSP
                     LFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLSARFNS
                     AMDIPAQGLCRIPEKRS"
     gene            371..>1173
                     /locus_tag="POB34_004961"
     CDS             371..>1173
                     /locus_tag="POB34_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI5921768.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKVAPDKA
                     TFARVTAPLWQYLDALHPYLWRKGKDFPPSPARMDALLKAGTLRLSLTF"
BASE COUNT          257 a          334 c          333 g          249 t
ORIGIN      
        1 atcgcttgtc agcgcgcgca ttgcgcaaat gtgcctgtgt gagttttgcg tggatatcac
       61 cagtatgaaa gtcgccgagc gtaccggcag caccgataaa ctgctggcag tggctgactg
      121 gcggcaaagc ccgctcttca gcgatgaaga acggctggcg ctggagtacg ccgaagccgc
      181 aagcgtaacg ccgccaacgg tcgatgatgc cctgcgcacc cgactggctg cgcattttga
      241 cgctcaggcg ctcaccgaac tgacggcatt gatcggcctg caaaacctgt cggctcgttt
      301 taattctgcc atggacattc ccgctcaggg gctgtgccgt attccggaaa aacgttctta
      361 aggagagaag atgcgccatt gtgggtggtt gctgggattg ttatcgctgt tttctctggc
      421 aacacatgcc agtgactggc aggaaattaa aaatgaggcc aaagggcaaa ccgtctggtt
      481 taacgcctgg ggcggcgata ccgcaattaa ccgctatctc gactgggtta gcggcgagat
      541 gaaaacccat tacgctataa acctgaagat tgttcgtctg gcggatgccg cagacgcggt
      601 gaagcgcatt cagaccgaag ctgctgccgg acgtaaaacg ggcggctcgg tggatctgct
      661 ctgggtgaac ggcgaaaact tccgcacctt aaaagaggcc aatttattac aaacgggctg
      721 ggcggagact ctgcccaact ggcgctatgt cgacacacag ctgccggtgc gggaagattt
      781 ttcagtgcct acagaagggg ctgaatcgcc ctggggcggc gcacaactga cgtttatcgc
      841 ccgccgcgat gttacgccac agccaccaca aacgccgcaa gccttactgg agtttgctaa
      901 agccaatccc ggcacggtta cctatccgcg cccaccggac tttaccggca cggcgtttct
      961 tgaacagttg ctgattatgc tgacgcccga tcccgccgcg ttaaaagttg ccccggataa
     1021 agccactttc gcccgcgtca ctgcgccctt gtggcaatat cttgacgcgc tacatcccta
     1081 tttatggcgc aaaggaaagg atttcccgcc ttcgcccgcg cggatggatg ctctgctgaa
     1141 agccggcaca ttgcgcctgt cgctgacctt taa
//
LOCUS       DAMZET010000385         1160 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.388, whole genome shotgun sequence.
ACCESSION   DAMZET010000385 DAMZET010000000
VERSION     DAMZET010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.388"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..341
                     /locus_tag="POB34_004962"
     CDS             <1..341
                     /locus_tag="POB34_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HDI5921769.1"
                     /translation="RQASEADLQPGTIEYERHRLTRAQADAQELKNARDSAEVVETAF
                     CTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMNKAAALDELIPG
                     LLSEYIEQSG"
     gene            316..>1160
                     /locus_tag="POB34_004963"
     CDS             316..>1160
                     /locus_tag="POB34_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDI5921770.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGKEQYLKFGDKETPFGLKWMPDDPSSVFYLCEH
                     NACVIR"
BASE COUNT          271 a          296 c          333 g          260 t
ORIGIN      
        1 tgcggcaggc cagcgaggca gatctccagc cagggactat tgagtacgaa cgccatcgac
       61 ttacgcgtgc gcaggccgac gcacaggaac tgaagaatgc cagagactcc gctgaagtgg
      121 tggaaaccgc attctgtact ttcgtgttgt cgcggatcgc aggtgaaatt gccagtattc
      181 tcgacgggat ccccctgtcg gtgcagcggc gttttccgga actggaaaac cgacatgttg
      241 atttcctgaa acgggatatc atcaaagcca tgaacaaagc agccgcgctg gatgaactga
      301 taccggggtt gctgagtgaa tatatcgaac agtcaggtta acaggctgcg gcattttgtc
      361 cgcgccgggc ttcgctcact gttcaggccg gagccacaga ccgccgttga atgggcggat
      421 gccaattact atctcccgaa agaatccgca taccaggaag ggcgctggga aacactgccc
      481 tttcagcggg ccatcatgaa tgcgatgggc agcgactaca tccgcgaggt gaatgtggtg
      541 aagtctgccc gtgttggtta ttccaaaatg ctgttgggtg tttatgccta cttcatagag
      601 cataagcagc gcaacacact tatctggttg ccgacggatg gtgatgccga gaactttatg
      661 aaaacccacg ttgagccgac catccgtgat attccttcgc tgctggcgct ggccccgtgg
      721 tatggcaaaa agcaccggga taacacgctc accatgaagc gtttcaccaa tgggcgtggc
      781 ttctggtgcc tgggcggtaa agcggcaaaa aactaccgtg aaaagtcagt ggatgtggcg
      841 ggttatgatg aacttgctgc ctttgatgag gatattgaac aggaaggctc tccgacgttc
      901 ctgggcgata agcgtattga aggctctgtc tggccaaaat ccatccgtgg ctccacgccc
      961 aaagtgagag gcacctgcca gattgagcgt gcagccagtg aatccccgca ttttatgcgt
     1021 tttcatgttg cctgcccgca ctgcgggaag gagcagtacc ttaaatttgg cgacaaagag
     1081 acgccgtttg gcctcaaatg gatgccggat gatccctcca gcgtgtttta tctctgtgag
     1141 cataatgcct gcgtcatccg
//
LOCUS       DAMZET010000386         1152 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.389, whole genome shotgun sequence.
ACCESSION   DAMZET010000386 DAMZET010000000
VERSION     DAMZET010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.389"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            56..850
                     /locus_tag="POB34_004964"
     CDS             56..850
                     /locus_tag="POB34_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5921771.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            872..>1152
                     /locus_tag="POB34_004965"
     CDS             872..>1152
                     /locus_tag="POB34_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDI5921772.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPL"
BASE COUNT          232 a          281 c          340 g          299 t
ORIGIN      
        1 ccgattgttc tcatcgcact gtacctggcg ttcgtgaaac gtctgggagc gttcgatgca
       61 ctctgaacgc gcaccgtttt tcctcaaact ggcggcctgg ggcggcgtgg ttttcctaca
      121 ttttcccatc ctgattatcg ccgcttatgc gtttaacact gaagatgcgg cgtttagttt
      181 tccaccgcag ggcctgacgc tgcgctggtt tagcgtggca gcacagcgta gtgatattct
      241 tgatgccgtg acactgtcac ttaaagtggc ggcgctggcg acattaatgg cgctggtgtt
      301 agggacgctg gcagctgccg cgctgtggcg acgagacttt ttcggcaaaa acgccatttc
      361 gctgttactg ctgctgccca ttgcgctgcc gggcattgtc actggtctgg cgctattaac
      421 cgcttttaaa accatcaatc tggagccggg atttttcacc atcgtggtcg gtcatgcgac
      481 tttttgcgta gtggtggtgt ttaacaatgt catcgcccgt tttcgccgca cctcgtggag
      541 tctggttgag gcgtcaatgg atcttggggc caatggctgg caaaccttcc gctacgtagt
      601 gttgccgaat ctcagttcgg cgctactggc aggaggaatg ctggcgtttg ccttgtcgtt
      661 cgatgaaatc atcgttacga cttttacggc aggtcatgaa cgaacgttac cgttgtggtt
      721 gctcaatcag cttgggcgac cgcgtgatgt accggtaact aacgtggtgg cactgctggt
      781 tatgttggta acaaccttgc cgatcctggg ggcctggtgg ctaacccgcg aaggcgacaa
      841 tggtcaataa ccactgatac aggaatatgc tatgcaacat aagttactga ttaacggaga
      901 actggttagc ggcgaagggg aaaaacagcc tgtctataat ccggcaacgg gggacgtttt
      961 actggagatt gccgaggcat ccgcagagca ggtcgatgct gctgtgcgcg cggcagatgc
     1021 agcatttgcc gaatgggggc aaaccacgcc gaaagtgcgt gcggaatgtc tgctgaaact
     1081 ggctgatgtt atcgaagaaa atggtcaggt ttttgccgaa ctggagtccc gtaattgtgg
     1141 caaaccgctg ca
//
LOCUS       DAMZET010000387         1137 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.390, whole genome shotgun sequence.
ACCESSION   DAMZET010000387 DAMZET010000000
VERSION     DAMZET010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.390"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            101..>1137
                     /locus_tag="POB34_004966"
     CDS             101..>1137
                     /locus_tag="POB34_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HDI5921773.1"
                     /translation="MSGKPAARQGDATMKGGPIVQGSAGVRIGAPTGVACSVCPGGFT
                     SGNPVNPLLGAKVQPGETDFALPGPLPFVLSRTYSSYRTKTPAPVGIFGPGWKAPFDI
                     RLQIRDNELILNDNGGRSIHFEHLFPGEDGFSRSELFWLVRGGVAKLNESHRLAPLWQ
                     ALPEELRLSPHIYLATNSPQGPWWVLGWSERVPGGNEVLPAPLPPYRVLTGLVDRFGR
                     TLTFRREAACEFTGEITGVTDSAGRQFRLVLTTQAQRAENARQQAIAAGAKGPDIPDS
                     LPDYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYDWTPRGELAAVYDRRGTQMR
                     HFTYDDKYRGR"
BASE COUNT          236 a          331 c          353 g          217 t
ORIGIN      
        1 atgtaaaaag agcgtatttc gtttcataca gtaacgttat ttatcatttt gtccggtgcc
       61 gccagcatca ggccagatga accataaaag gatatcagcg atgagcggaa aaccagcggc
      121 acgtcagggc gatgccacaa tgaaaggtgg cccgatagtc caggggtcgg caggcgtgag
      181 aattggtgcg ccaaccggcg tggcctgctc agtgtgcccc ggcggattca cttccggcaa
      241 cccggtgaat ccgctgctcg gtgcaaaagt acagcccggc gaaaccgatt tcgcgctgcc
      301 cggcccgtta ccgttcgtgc tctcccgcac ctacagcagc taccggacga aaacccctgc
      361 gcccgtcggt atcttcggtc ctggctggaa agcacccttt gatatccgct tacagatacg
      421 cgataacgaa ctgatactca atgataacgg tggcagaagc attcattttg agcacctgtt
      481 tcccggtgag gatggtttca gccgcagcga attattctgg ctggtacgcg gcggcgtggc
      541 gaaactgaat gaaagtcacc gtcttgcccc actctggcag gctctgccgg aagaactccg
      601 cctgagtccg catatttatc tggcgacgaa cagtccgcag gggccgtggt gggtactcgg
      661 ctggagtgag cgggtgccgg gggggaatga ggtgctgcct gctccgctgc cgccgtaccg
      721 ggtgctgacg ggcctggtgg accgcttcgg gcgtaccctg accttccggc gtgaggccgc
      781 atgcgaattt accggggaaa tcaccggcgt gaccgatagc gccggccgtc agttccgtct
      841 ggtgctgacc acgcaggcac aacgggcaga aaacgcccgg cagcaggcca tcgccgcagg
      901 agcgaaagga ccggatatcc cggattcact gccggactat acggagtatg gccgggataa
      961 cggtatccgc ctgtcagcgg tgtggctcac gcacgacccg gaatacccgg agaatttacc
     1021 tgccgcgcca ctggtgcgct acgactggac gccacgcggt gaactggcgg cggtgtatga
     1081 ccgccgcggt acgcagatgc gccactttac ttacgatgat aaataccggg gccggat
//
LOCUS       DAMZET010000388         1137 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.391, whole genome shotgun sequence.
ACCESSION   DAMZET010000388 DAMZET010000000
VERSION     DAMZET010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.391"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..347)
                     /gene="nemR"
                     /locus_tag="POB34_004967"
     CDS             complement(<1..347)
                     /gene="nemR"
                     /locus_tag="POB34_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HDI5921774.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEV"
     gene            450..689
                     /locus_tag="POB34_004968"
     CDS             450..689
                     /locus_tag="POB34_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HDI5921775.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            738..>1137
                     /locus_tag="POB34_004969"
     CDS             738..>1137
                     /locus_tag="POB34_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDI5921776.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSTEQSLINLATDHLDLLLIHRPDPL"
BASE COUNT          273 a          266 c          306 g          292 t
ORIGIN      
        1 cacacttcgg cagagagttt tactgtcagg caaccactga tggttccatg ttggcaaaac
       61 tggttcagtg tttgctggta ataagccagt atgcggtcgc ggtagttacc ttcgccggat
      121 tgcagcaact cagtcagtcg ctggtgatat gcggcgtaat gacgctcaag catggcaacg
      181 ccaaacgctt ctttagagcg aaagtagtga tagaaggacc ctttcggcac ttcagcggtt
      241 tttagtaatt cgcttagccc catcccggtg aatccacgtt gcaggcaaag ttgctcgccc
      301 gtcgccagga gatgttcgcg agtatcatgt tcggtgtgtt tgttcatgcg ttggagtgta
      361 gtagaccagt cggtctagtg caagcaaagt tgctactttg gacattaaca gcttcaataa
      421 tggtaataat cttatctgag gagggcgaag tggcggagca attagagttc tttcccgtcc
      481 agagcccgtg ccggggaatt tgccagtctg acgaacgcgg gttttgtcgc ggctgttttc
      541 gtagtcgtga tgagcgtttt aactggaata aaatgagcga tggcgaaaaa caagaggtgc
      601 tgcgcctttg tcggcagcga ttaatgcgca aattacgcgc aaataagcca gcgtcatcag
      661 acgaaccgga gcaaccatca ctcttttgaa cctaatcttg cgtatactcc tctcatatct
      721 gataagagga agcgattatg gttcagcgta ttactattgc gccgcaaggc ccggagtttt
      781 cccgttttgt gatgggctac tggcgattga tggactggaa tatgtccgcc cgccagctgg
      841 tcagttttat tgaagagcat ctggatctcg gcgtgaccac cgtggaccat gctgatattt
      901 atggtggcta tcagtgcgaa gcggcgtttg gcgaggcact gaaactggca cctcacctgc
      961 gtgaacggat ggaaatcgtc agtaaatgcg gtatcgcgac gaccgcgcgt gaagaaaacg
     1021 tcattggtca ttacatcact gaccgcgatc acatcattaa gagcaccgaa cagtcgctaa
     1081 ttaatctcgc gaccgatcat ctggatctgc tgttaatcca ccgaccagac ccgttaa
//
LOCUS       DAMZET010000389         1107 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.393, whole genome shotgun sequence.
ACCESSION   DAMZET010000389 DAMZET010000000
VERSION     DAMZET010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.393"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..347
                     /locus_tag="POB34_004970"
     CDS             <1..347
                     /locus_tag="POB34_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDI5921777.1"
                     /translation="TVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNL
                     KASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVM
                     MKFHQWFGSWFS"
     gene            complement(394..>1107)
                     /gene="deoR"
                     /locus_tag="POB34_004971"
     CDS             complement(394..>1107)
                     /gene="deoR"
                     /locus_tag="POB34_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HDI5921778.1"
                     /translation="LKRSDKLHLKDAAALLGVSEMTIRRDLNNHSAPVVLLGGYIVLE
                     PRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGTTTPWIIEAIDNEIPF
                     TAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQTLNNFCPDIAFYSAAG
                     VHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPARMGDLKRFDIVVSDC
                     CPEDEYVKYAQTQRIKLMY"
BASE COUNT          283 a          273 c          287 g          264 t
ORIGIN      
        1 aaaccgtgac gccaattaaa cctgatgcca cctttgtgac tcagcgcgtc tggtttggtg
       61 ataagagcga agtgaatctt ggggcaggcg aagcgggctc agtgaccata ccgcgtgggc
      121 agctgaaaaa cctgaaggcg agctacacgt taacggaacc gcagcttacc gcaccgctga
      181 aaaaaggtca ggttgtcggg accattgatt tccagcttaa cggtaaatcc attgagcagc
      241 gtccgctgat cgtgatggaa aatgtggaag agggcggatt ctttggtcgg atgtgggatt
      301 tcgtgatgat gaaattccat cagtggttcg gcagctggtt ctcttaatct tctgataacc
      361 ggatggcgcg aaacgtcatc cggttatacg tcattaatac atcaacttaa tgcgctgcgt
      421 ctgcgcgtac ttcacatact catcttccgg gcaacaatca ctcaccacaa tatcaaagcg
      481 tttcaggtca cccatgcgcg ccggacgcac cttgccaaat ttactgtggt cgacaaccag
      541 cacatgcttt tgcgccatcg acatggccca gtgttttacc ggcaactctt caagattaaa
      601 acaggtagcg cctttactga catgcacgcc cgccgcagaa taaaaagcga tatccgggca
      661 aaaattattc agcgtttgct gaaaatcgat gggtttgaaa atggcgttgc tggcgtgaaa
      721 ttcaccaccg cacagaaatg cgcggcaatg gggtttctct ttcagcgcca gaaaggtatt
      781 tagcgaataa caaacggcgg taaaagggat ttcattatca atcgcttcaa taatccacgg
      841 cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt tctaccagtg tcgcagccag
      901 ttttgccgcc cggcgttttt cttccaccag gcgggatttt tgatcgctta acaggtaatg
      961 gctggcactg cgcggttcca gaacaatata gccgccgagc aaaacgacgg gcgcactgtg
     1021 gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca agcagggcgg cggcgtcttt
     1081 aagatgtaac ttatcgctgc gttttaa
//
LOCUS       DAMZET010000390         1088 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.394, whole genome shotgun sequence.
ACCESSION   DAMZET010000390 DAMZET010000000
VERSION     DAMZET010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.394"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..954
                     /locus_tag="POB34_004972"
     CDS             <1..954
                     /locus_tag="POB34_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HDI5921779.1"
                     /translation="EVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKL
                     LQESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVL
                     DGKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVM
                     LSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTI
                     AALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATER
                     ELQYTSEVLGLKYDKPQAADSTPKPQLKPKPVQKEVADIAL"
BASE COUNT          258 a          261 c          332 g          237 t
ORIGIN      
        1 gaagtccgcg ccctaaagcg taatgacgat aacacctgga ccgttaccgt tgccgatctg
       61 aaaaatggca ctgcacagaa cattcgtgcc aaatttgtct ttatcggcgc gggcggtgcg
      121 gcgctgaagc tgttacagga atcggggatt ccggaagcga aagactacgc cggtttcccg
      181 gtgggcggac agttccttgt ttcggaaaac ccggacgtgg ttaatcacca tctggcgaag
      241 gtttacggta aagcatccgt tggcgcacca ccgatgtcgg ttccgcatat cgatacccgc
      301 gttctggacg gtaaacgcgt agtgctgttt gggccatttg ccaccttctc aaccaaattc
      361 ctcaaaaacg gttcattgtg ggatctgatg agttccacca ccacctctaa cgtgatgccg
      421 atgatgcacg tcgggctgga taatttcgat ctggtgaaat atctggtgag tcaggtgatg
      481 ttgagtgaag aggatcgttt tgaagcgttg aaagagtact atccgcaggc gaaaaaagag
      541 gactggcgtt tgtggcaagc ggggcagcgc gtgcagatta tcaagcgtga tgccgagaaa
      601 ggtggcgtac tgcgtctggg tactgaagtc gtcagtgacc agcaagggac cattgccgcg
      661 ctcctggggg catcgccagg ggcgtcaacc gccgcgccga ttatgttgga tctgctggaa
      721 aaagtatttg gcgatcgcgt ttccagcccg caatggcagg ctacgttgaa agcgatcgtt
      781 ccgtcttatg gacgcaagct gaacggtgat gtagcggcaa cagaacgcga gttgcagtac
      841 accagcgaag tgctggggct gaaatacgac aagccgcagg cagcagatag tacgccgaaa
      901 ccgcagttga aaccgaaacc cgttcaaaaa gaagtggcgg atattgcgtt gtaatgatgt
      961 gccacatccg gcatggtatg ccggggatgt ggcgtatgct gataagacgc gccagcgtct
     1021 tatcaggcaa ccggctcggg cgttagatgt cggatgcggc gtaaccgcct tatccaatct
     1081 acggggtg
//
LOCUS       DAMZET010000391         1082 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.395, whole genome shotgun sequence.
ACCESSION   DAMZET010000391 DAMZET010000000
VERSION     DAMZET010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.395"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..112
                     /locus_tag="POB34_004973"
     CDS             <1..112
                     /locus_tag="POB34_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDI5921780.1"
                     /translation="EAKVNNNLSLWGNVGVQLGDKGYSDTQGMLGVKYSW"
     gene            321..587
                     /gene="hipB"
                     /locus_tag="POB34_004974"
     CDS             321..587
                     /gene="hipB"
                     /locus_tag="POB34_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HDI5921781.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            587..>1082
                     /gene="hipA"
                     /locus_tag="POB34_004975"
     CDS             587..>1082
                     /gene="hipA"
                     /locus_tag="POB34_004975"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HDI5921782.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGN"
BASE COUNT          325 a          236 c          262 g          259 t
ORIGIN      
        1 tgaggcgaaa gtaaataaca accttagcct gtgggggaat gtcggtgtgc aactaggtga
       61 taaaggctat agcgatactc agggcatgct gggagtgaaa tatagctggt aaaccgtata
      121 agccgcatgt cgagatggca tgcggcttaa tattgccgac ttcaaaggtt ttaaagcctc
      181 ccttttatcc gtgatcgcgg atatcgcagc gtttatcccg tacagcggat aagatgtgtt
      241 tccagattga ctttatcccc actaaaggat aaaactgata atatcccctt aagcggataa
      301 atttgctgtg gacgtatgac atgatgagct ttcagaagat ctatagccca acgcaattag
      361 cgaatgcaat gaaactggtt cgccagcaaa atggctggac gcagagcgag ctggcgaaaa
      421 aaattggcat taagcaggcg acaatttcca atttcgaaaa caaccctgac aataccacgc
      481 tcacgacatt ttttaagatt ttacagtcgc ttgaactctc aatgacacta tgcgacacga
      541 aaaatgcctc gccagaatca acagaacagc aagatctgga gtggtaatgc ctaaacttgt
      601 cacttggatg aacaaccagc gggtaggcga gttaacgaag ttagccaacg gcgcgcacac
      661 ctttaagtat gcaccggagt ggttagccag ccgttatgcc agaccgttgt cactttcgct
      721 gccattgcag agggggaata tcacctcaga tgccgtattt aacttcttcg ataacctgtt
      781 acccgatagc ccgattgtac gtgaccggat cgttaaacgt tatcatgcta aatccagaca
      841 accgtttgat ttattgtcag aaatagggcg agacagcgtt ggtgccgtaa cgttattacc
      901 cgaaaatgaa accataacgc gcccgataat ggcatgggaa aagcttactg aagccaggct
      961 tgaagaagta ttaacggctt ataaagcaga tatccctctg ggcatgatta gagaagaaaa
     1021 tgactttcgc atctcggttg ctggcgcgca ggagaagaca gcgctgctta gaataggcaa
     1081 tg
//
LOCUS       DAMZET010000392         1041 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.396, whole genome shotgun sequence.
ACCESSION   DAMZET010000392 DAMZET010000000
VERSION     DAMZET010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.396"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            110..262
                     /gene="hokE"
                     /locus_tag="POB34_004976"
     CDS             110..262
                     /gene="hokE"
                     /locus_tag="POB34_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HDI5921783.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(384..1004)
                     /gene="entD"
                     /locus_tag="POB34_004977"
     CDS             complement(384..1004)
                     /gene="entD"
                     /locus_tag="POB34_004977"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HDI5921784.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
BASE COUNT          259 a          247 c          257 g          278 t
ORIGIN      
        1 gtctggaaag atgaagcccc aggagatatt tctatcaacc ctggggctgc cactccaaac
       61 ccgaacaatt tggatggtag tcccttcttc gcatggaggc aatataaaca tgctgacgaa
      121 atatgccctt gcggcagtca tagtgctgtg tttaacggtg ctgggattta cgcttctggt
      181 cggagattcg ctgtgtgagt tcaccgtaaa ggaacgtaat attgagttta aggctgttct
      241 cgcttacgaa ccgaagaagt agccgttgtg cggggagcaa tccccgcata tccggttgtc
      301 aggtcaggat ggtaaggcac ctgctttaca ctttcgcccg tggtcagtga tggctgcggg
      361 cgaatcgtac cagatgttgt caattaatcg tgttggcaca gcgttatgac tatcttttct
      421 tttatctgcc agtgcacagc aaacatctca ttctcacgat gaatgatgac ctgctgttta
      481 ttccagctaa ttatctgata gtccagaaaa cctgcatcag tttggatctc acttgcctta
      541 aatgcgctct ctttggcgga aaatgccagt gtcagcgcca gagaaaaggc taaaccgcag
      601 tctgcgagtc gctcgtgttc cgctggtgta ataatgttgt ctgtcaattc tcttgcggtt
      661 tgtacagaaa aaatttcttc tatatcaatg ccaatcggtt gacgagatac cacggctaat
      721 gccgtagtcc cacagtggct aatactgccg tatacctccg caggccagac aggttggcgt
      781 agctcgccga ttgcgggcac acatttatag ccatattccc gcaaagcata aacagcagcg
      841 atccgtccgg ctaaatgctc tgttttacgt ttacgtccag cgtgttgcag ttgtgcgtag
      901 tgcggcagcc agagtaaatc ctgctcacaa aaattcgccg gatcgaactc aacaaaatgc
      961 agcgtatgtc cggcaaaggg gagggaggta tgcgtagttt tcatatcgac catattcgag
     1021 actgatgaca aacgcaaaac t
//
LOCUS       DAMZET010000393         1025 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.397, whole genome shotgun sequence.
ACCESSION   DAMZET010000393 DAMZET010000000
VERSION     DAMZET010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.397"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(165..>1025)
                     /gene="gsiD"
                     /locus_tag="POB34_004978"
     CDS             complement(165..>1025)
                     /gene="gsiD"
                     /locus_tag="POB34_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HDI5921785.1"
                     /translation="VKPDQVRTPWHEFWRRFRRQHMAMTAALFVILLIVVAIFARWIA
                     PYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQISLAAGVFAVFIGAAIG
                     TLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVLGSGIANVIIAVAIFSIP
                     AFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGTVSSIVVFFTMRIGTSII
                     SAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPALAIFLTVLAFNLLGDGL
                     RDALDPKIKG"
BASE COUNT          310 a          286 c          237 g          192 t
ORIGIN      
        1 acaaaataac tatctgaacg ctgtgccgct gcaaaaatag tcccttgctg cggcttttta
       61 accaaattac ccgattctac aaaatcagca gaaagttaca acacttgctt agggcaataa
      121 ttgcctgtga ggtgattacc ctttcaagca atattcaaac gtaactatcc tttaattttc
      181 ggatccagcg catcgcgtaa accatcgccc aacaaattga acgccagtac ggtcagaaaa
      241 atagccaggg ccggaaaaac agcgacatgc ggcgcgataa ccatatccgc tcgtgcctca
      301 ttgagcattg ctccccactc tggtgtcggc ggctgcgcac cgaggccgag aaatgacagg
      361 ctggcggcag agataatcga ggtgccaatg cgcatggtga aaaacaccac gatagacgag
      421 acggtcccag gcaagatatg acgcaataaa atggtcatat cgctggcacc aatactgcgt
      481 gctgactcaa taaaggtttg ctgtttcaac accagcgtgt tgccgcgcac caggcgggca
      541 aacgcgggga tggaaaaaat ggcgactgcg ataatcacgt tagcaatgcc gcttcccaac
      601 accgcaacaa cagcgatcgc cagtaaaata cccgggaagg caaacagcac atcgcaaatg
      661 cgcatgatca gccgatccca ccagccttca taatatccag ccagcaagcc cagcaacgtc
      721 cctatcgccg caccgataaa cacggcaaac acgcccgccg ccagcgagat ttgcgcacca
      781 accaggacac ggctgaaaat gtcgcgcccc agtgaatcga cgccaaacca gtgctgcaaa
      841 gaaggtccgt tattcagatt gtcataatca aaataatttt cggcgtcata gggagcgatc
      901 cagcgtgcaa aaatggccac cacaatcaat aaaataacga ataatgcggc ggtcatcgcc
      961 atatgctgac ggcgaaatcg tcgccagaat tcatgccacg gtgtacgcac ctggtcaggt
     1021 ttgac
//
LOCUS       DAMZET010000394         1024 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.398, whole genome shotgun sequence.
ACCESSION   DAMZET010000394 DAMZET010000000
VERSION     DAMZET010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.398"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..447
                     /locus_tag="POB34_004979"
     CDS             <1..447
                     /locus_tag="POB34_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI5921786.1"
                     /translation="TLAILYYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITL
                     EQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGY
                     ENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            complement(490..>1024)
                     /locus_tag="POB34_004980"
     CDS             complement(490..>1024)
                     /locus_tag="POB34_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="may play a role in electron transport between the
                     anaerobic fatty acid oxidation pathway and the respiratory
                     chain; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDI5921787.1"
                     /translation="STCEVYLDNVEVEESDMVGEEGMGFLNVMYNFEMERLINAARST
                     GFAECAFEDAARYANQRIAFGKPIGHNQMIQEKLALMAIKIDNMRNMVLKVAWQADQH
                     QSLRTSAALAKLYCARTAMEVIDDAIQIMGGLGYTDEARVSRFWRDVRCERIGGGTDE
                     IMIYVAGRQILKDYQNK"
BASE COUNT          261 a          246 c          226 g          291 t
ORIGIN      
        1 acgttagcca ttctttacta cgaaaatttt aaaaatgctt atcgttcgga gcaggggtat
       61 attaaaaaag atgttctgat aaaagatatt cttttctatc tgaataataa ttatcgcgaa
      121 aaaatcactt tagaacagtt atcgaaaaaa tttcgtgcca gcgtcagtta tatttgccat
      181 gaatttacca aagagtatcg tatttcccct attaactatg ttattcaacg gcgtatgacg
      241 gaagcaaaat ggtcactcac taatactgaa ttatcacagg cagagatctc ctggcgtgtg
      301 ggttatgaaa atgtcgatca ctttgccaaa ctgtttttgc gccatgtcgg ttgttcgccc
      361 agcgattacc gtagacaatt taaaaactgt tttgcggaac aagaaatcct atctgaattt
      421 cctcaaccgg taagtcttgt tggataaatc tggcggcgca gtttgttaag aagctgcgcc
      481 gcctgcagat tatttgttct gatagtcttt caggatctgc cgacctgcta cgtaaatcat
      541 aatttcgtct gtaccgccgc cgatacgttc acaacggaca tcacgccaga agcgggagac
      601 gcgcgcttca tcggtatagc ccagaccgcc catgatttga atcgcatcat caatgacttc
      661 cattgcggta cgtgcgcaat acagttttgc cagcgccgcg ctggtgcgca gtgactgatg
      721 ctgatcggct tgccatgcca ctttcagcac catgttgcgc atgttgtcga tcttaatcgc
      781 catcagcgcc agtttttcct ggatcatctg gttatgacca atgggcttac caaaagcgat
      841 acgttggttg gcatagcggg cggcatcttc aaaggcgcat tcggcaaagc cggtgctgcg
      901 cgcggcgttg atcaggcgct ccatctcaaa gttgtacatc acattgagga aacccattcc
      961 ttcttcgccc accatgtcgc tctcttcaac ctccacgttg tcgagataga cttcgcaggt
     1021 gctg
//
LOCUS       DAMZET010000395         1015 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.399, whole genome shotgun sequence.
ACCESSION   DAMZET010000395 DAMZET010000000
VERSION     DAMZET010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.399"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(48..155)
                     /locus_tag="POB34_004981"
     CDS             complement(48..155)
                     /locus_tag="POB34_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDI5921788.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(531..638)
                     /locus_tag="POB34_004982"
     CDS             complement(531..638)
                     /locus_tag="POB34_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDI5921789.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT          213 a          307 c          238 g          257 t
ORIGIN      
        1 tccgggcgca aattgccatt acggcagcct gacgcccgca tgacacgtta cttccgcttg
       61 ttcagccagt tcacgatcat actggcaaga atgccagcaa tgaccggagc cgctaaatca
      121 tgccagaagg ccatgcccag ttctgcgagc gtcatatagc cgcctgtgtt gtaatgacaa
      181 cgtttcgcgg ctattcttga gttgttcagg ttcaagaatg gcccccgtga tgttgtcagg
      241 tgcatacctg caacgtgcgg gggttttctc tctccagcaa ccaatgccac cagggataaa
      301 gcccccgcaa cattgcgcct caccggaacc cttccggctt gacgctgatt ctacgactgt
      361 ttttctgttc cggaatccat cacttactca ctctgtgctg cgcattccgc agaatattat
      421 ccgcttgcaa ccgttgcatt tttcttacga aaaaggccgc agagtttcac ccctgcggcc
      481 tgatccgggc gcaaattgcc attacggcag cctgacgccc gcatggcacg ttacttccgc
      541 ttgttcagcc agttcacgat catactggca agaataccag caatgaccgg agccgctaaa
      601 tcatgccaga aggccatgcc cagttctgcg agcgtcatat agccgcctgt attgtaatga
      661 caacgtttcg cggctattct tgtattgttt ggatgcaaga atggcccccg tggtgttgtc
      721 aggtgcatac ctgcaacgtg cgggggtttt ctctctccag caaccaatgc caccagggat
      781 taagcccccg caacattgcg cctcaccgga atccttccgg cttgacgctg attctacgac
      841 tgtttttctg ttccggaatc cgtctcatct tcactctgtg ctgcgcattc cgcagaatat
      901 tatccgcttg caaccgttgc atttttctta cgaaaaaggc cgcagagttt cactcctgcg
      961 gcctggtccg ggcgcaaatt gccattacgg cagcctgacg cccacatgac acgct
//
LOCUS       DAMZET010000396         1014 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.400, whole genome shotgun sequence.
ACCESSION   DAMZET010000396 DAMZET010000000
VERSION     DAMZET010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.400"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..969
                     /gene="cusB"
                     /locus_tag="POB34_004983"
     CDS             <1..969
                     /gene="cusB"
                     /locus_tag="POB34_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HDI5921790.1"
                     /translation="PTQTQNLGVKTATVTRGPLTFAQSFPANVSYNEYQYAIVQARAA
                     GFIDKVYPLTVGDKVQKGTPLLDLTIPDWVEAQSEYLLLRETGGTATQTEGILERLRL
                     AGMPEADIRRLIATQKIQTRFTLKAPIDGVITAFDLRAGMNIAKDNVVAKIQGMDPVW
                     VTAAIPESIAWLVKDASQFTLTVPARPDKTLTIRKWTLLPGVDAATRTLQLRLEVDNA
                     DEALKPGMNAWLQLNTASEPMLLIPSQALIDTGSEQRVITVDADGRFVPKRVAVFQAS
                     QGVTALRSGLAEGEKVVSSGLFLIDSEANISGALERMRSESATHAH"
BASE COUNT          224 a          283 c          299 g          208 t
ORIGIN      
        1 ccgactcaga cgcagaatct gggggtgaaa acggctaccg tcacgcgcgg accgctgact
       61 tttgcccaga gtttcccggc gaatgtcagt tacaacgagt atcagtatgc cattgtgcag
      121 gctcgcgctg ccgggtttat cgacaaggtg tatccgctta ccgtgggcga taaagtacaa
      181 aagggcacac cgcttctcga cctgaccatt cctgactggg tggaagcgca gagtgagtat
      241 ttactgctgc gcgaaaccgg cggtacggcg acccagactg aaggcattct tgagcggctg
      301 cgactggcgg gaatgccgga ggcggatatt cgccgcctga tcgccacgca aaaaatccag
      361 actcgcttta cgctcaaagc gcccattgat ggcgtgatca ccgcgtttga tctgcgcgcg
      421 ggaatgaata tcgccaaaga taacgtggtc gcgaaaattc agggtatgga cccggtgtgg
      481 gtcactgctg cgatcccgga gtccatcgcc tggctggtga aagatgcctc gcagtttacc
      541 ctcaccgttc cggcgcgacc ggataaaaca ctcaccatcc gcaaatggac gctgctacct
      601 ggcgtggatg ccgcgacccg cacgctgcag ctgcgtctgg aagtcgacaa cgccgacgag
      661 gcgctaaaac cgggcatgaa cgcctggttg caactcaaca ccgccagcga accgatgctg
      721 ctcattccgt cacaagcgct gattgatacc ggcagcgaac agcgggtgat taccgttgat
      781 gccgacgggc gctttgtacc gaaacgcgtt gctgttttcc aggcatcgca aggcgtcacc
      841 gcattacgct ctggtctggc ggaaggtgaa aaggtggttt ccagcggcct gttcctgatt
      901 gattccgaag ccaatatttc tggcgcactg gagcggatgc gctctgaaag tgctacccat
      961 gcgcattgag ggaataacca atgattgaat ggattattcg tcgctcggtg gcga
//
LOCUS       DAMZET010000397          968 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.402, whole genome shotgun sequence.
ACCESSION   DAMZET010000397 DAMZET010000000
VERSION     DAMZET010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.402"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>968
                     /gene="fliF"
                     /locus_tag="POB34_004984"
     CDS             <1..>968
                     /gene="fliF"
                     /locus_tag="POB34_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDI5921791.1"
                     /translation="GRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLTQSNTS
                     GRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTEEQYRP
                     NGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNNRQQQA
                     STTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGKPLPLS
                     NEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQLLAAG
                     RWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVE"
BASE COUNT          241 a          255 c          286 g          186 t
ORIGIN      
        1 ccggccgcgc actcgatgaa gggcaaatta gcgccattgt gcatctggtt tccagcgccg
       61 ttgctggtct gccgccggga aacgtcacgc tggtggatca gggcggacat ctgttaaccc
      121 agtccaatac cagcgggcgc gatcttaatg atgctcagtt gaaatatgcc agcgatgtcg
      181 aaggccgtat tcagcggcgt attgaagcaa tcctgtcgcc tattgttggt aacggtaata
      241 ttcacgccca ggtcacggcg cagctggact tcgccagtaa agaacaaacg gaagaacagt
      301 atcgccctaa cggtgatgaa tctcatgcgg cgcttcgttc acgccagctt aatgagagcg
      361 agcaaagcgg ttccggttat ccgggcggcg taccgggggc gttgtcgaat caaccggcac
      421 ctgcgaataa cgcgccaatc agcacgcctc cggcaaatca aaataaccgc cagcagcagg
      481 cgagtaccac cagcaatagc gggccgcgta gcacacagcg gaatgaaacc agtaactacg
      541 aagtcgatcg caccattcgt cataccaaaa tgaacgtggg cgatgtgcaa cgtctgtcag
      601 tcgcggtagt ggtgaattac aaaaccttgc cagacggtaa accattgcct ctcagcaacg
      661 aacagatgaa gcaaattgaa gatctgaccc gcgaggcgat gggcttttct gaaaaacgcg
      721 gtgactcgct caatgtcgtt aactcgccgt tcaatagcag tgacgaaagc ggcggagaac
      781 tgccgttctg gcaacagcaa gcgtttatcg atcagttgct ggctgccggt cgctggttgc
      841 tggtactgct ggtggcgtgg ctgctgtggc ggaaagcggt acgcccgcag ttaacacgtc
      901 gcgccgaggc gatgaaagct gtacagcaac aggcgcaggc ccgtgaggaa gtggaagatg
      961 cggtggaa
//
LOCUS       DAMZET010000398          966 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.403, whole genome shotgun sequence.
ACCESSION   DAMZET010000398 DAMZET010000000
VERSION     DAMZET010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.403"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            254..>966
                     /gene="ahr"
                     /locus_tag="POB34_004985"
     CDS             254..>966
                     /gene="ahr"
                     /locus_tag="POB34_004985"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HDI5921792.1"
                     /translation="MSMIKSYAAKEAGGKLEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINT"
BASE COUNT          226 a          229 c          287 g          224 t
ORIGIN      
        1 cacgtatttc tacggttgat tttgaatcaa ctgcgtctac cgatttcgcc acttcggcac
       61 tgaagaggta tgcggaaaac gttgtggatt atacctgtcg cacgccacca tgcaagcgcc
      121 agcttgctcg cacctcgcta agtgctgaaa ttttcagcat tgcatacagc gatgtgtaac
      181 ctttgtcaca ctccaggcac cccgccctac catgctctac acttcccaga caacaccaga
      241 gaaggacaaa aatatgtcga tgataaaaag ctatgccgca aaagaggctg gcggtaaact
      301 ggaagtttat gagtacgatc ccggtgaact gaaaccacaa gatgtcgaag tgcaggtgga
      361 ttactgcggg atctgccatt ccgatctgtc gatgatcgac aacgaatggg gattttcaca
      421 gtatccgctg gttgccgggc atgaggtgat tgggcgcgtg gtggcgctcg ggagcgccgc
      481 gcaggataaa ggtttgcagg tcggtcagcg tgtcgggatt ggctggacgg cgcgtagctg
      541 tggtcactgc gacgcctgta ttagcggtaa tcagatcaac tgtgagcaag gtgcggtgcc
      601 gacgattatg aatcgtggcg gctttgctga gaagttgcgt gcggactggc aatgggtgat
      661 tccactgcca gaaaatatcg acattgaatc tgccgggcca ctgttgtgcg gcggtatcac
      721 ggtctttaaa ccactgttga tgcaccatat cactgccacc agccgcgttg gggtaattgg
      781 tattggcggg ctggggcata tcgctataaa acttctgcac gcaatgggat gcgaggtgac
      841 ggcctttagt tcgaatccgg cgaaagagca ggaggtgctg gcgatgggtg ccgataaagt
      901 ggtgaatagc cgcgatccgc aggcactgaa ggctctggcg gggcagtttg atctcattat
      961 caacac
//
LOCUS       DAMZET010000399          954 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.404, whole genome shotgun sequence.
ACCESSION   DAMZET010000399 DAMZET010000000
VERSION     DAMZET010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.404"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            419..880
                     /locus_tag="POB34_004986"
     CDS             419..880
                     /locus_tag="POB34_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921793.1"
                     /translation="MATTLTSECNLLAVPFSAATDFTDLAEYCDRVATTLIECRDLAL
                     KTALCGRLNTCLALLRPTLNEPIPPHLIDGFITNDLPNVSPGFEPDAGSLCDYCLALT
                     QILNGHALLPETETTLTGLLCELVWYFAADLNAPRWIHTADGIQVIDGRPA"
     gene            877..>954
                     /locus_tag="POB34_004987"
     CDS             877..>954
                     /locus_tag="POB34_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5921794.1"
                     /translation="MTQPDWHPADIIAGLRKKGTSLAEVS"
BASE COUNT          210 a          252 c          237 g          255 t
ORIGIN      
        1 tcattttttt gactgatatc aacgggacga attaactgac aggatattta ctggaaagtg
       61 tctgcatatt gctcaatata tattttatat aaatataatt gtatttaata tttatcacgt
      121 gattgtattt tcctgtgcgt ctgcttccgc taaacctctt gccgcaaatt accgatcatt
      181 gtattgttgc gccgcgtctg caaaatcagg cgtcgggatt ggcatcctgt aatacttcac
      241 tgacaacaca atattttgtg ttgcgtatgg tcacatctca atggtgggat cgggcggggg
      301 cgtcgtaaga cgcgccggtg tcagtgaggc cggtaatgcc aactccgtcc ggtttcacca
      361 cccgtgagat tagcctctcc agtggctgga atatttatca ctcactggag acggccttat
      421 ggcgactact ctcacctctg aatgcaattt acttgctgtt ccctttagtg ccgctaccga
      481 tttcaccgat ctggcggagt actgcgatcg cgttgccaca acgctcatcg agtgccgcga
      541 tctggcgctg aaaacggcgc tctgtggcag gcttaatacc tgtcttgcac tgttgcggcc
      601 cacgctgaac gagccgatcc caccacatct gattgacggg tttattacga atgatcttcc
      661 caatgtttca cccggttttg agcctgatgc aggatcactc tgcgattact gcctggcgct
      721 gacgcagata ctgaacggcc acgcgctgct gccggaaacc gaaaccacgc tgaccgggct
      781 gctgtgcgaa ctggtctggt attttgccgc cgatctgaac gccccacgct ggatacatac
      841 tgctgacggc atacaggtta tcgacgggag gccagcatga cgcaacccga ctggcacccc
      901 gccgatatta tcgcgggcct gagaaaaaag ggaacatcgc tggctgaggt ttcc
//
LOCUS       DAMZET010000400          908 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.405, whole genome shotgun sequence.
ACCESSION   DAMZET010000400 DAMZET010000000
VERSION     DAMZET010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.405"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..901
                     /gene="yeaW"
                     /locus_tag="POB34_004988"
     CDS             <1..901
                     /gene="yeaW"
                     /locus_tag="POB34_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HDI5921795.1"
                     /translation="RGRDKVLRAFYNVCPHRGHQLLSGEGKAKNVITCPYHAWAFKLD
                     GNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPNATSVEDQLPGLGAKVLE
                     ACPEVQDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPGFSDSVQVDRYWHTMHGN
                     WTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPIKGMMTVIYEFPVDSETT
                     LQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQKGLKSRGYRGQGRIMADS
                     SGSGISEHGIAHFHNLLAQVFKD"
BASE COUNT          225 a          229 c          243 g          211 t
ORIGIN      
        1 acgcggtcgt gataaggttt tgcgcgcgtt ctataacgtg tgtccgcacc gtggtcatca
       61 gttgttgagc ggtgaaggaa aagcaaaaaa tgtgattacc tgcccgtatc acgcatgggc
      121 attcaaactc gatggcaatc tggcccatgc acgtaactgc gaaaacgtcg ccaatttcga
      181 tagcgacaaa gcgcaactgg ttccggtgcg tctggaagaa tatgccggat tcgtcttcat
      241 caacatggac cccaacgcca ccagcgtaga agatcagtta cccggtctgg gcgcgaaagt
      301 gctggaagcc tgcccggaag tccaagatct gaaactggcg gcccgcttta ccacccgcac
      361 gcctgccaac tggaagaaca ttgtcgataa ctatctcgag tgctatcact gtggtccggc
      421 gcatccaggt ttctccgact ccgtacaggt tgatcgttac tggcacacca tgcacggtaa
      481 ctggacgctg caatacggtt tcgccaaacc gtccgaacag tcgtttaaat ttgaagaggg
      541 tacggatgcg gcattccacg gtttctggct gtggccgtgc acgatgctga acgtcacccc
      601 gatcaaaggg atgatgacgg tcatttatga attcccggtg gattctgaaa ctaccctgca
      661 aaactacgat atttacttca ccaatgaaga gttaaccgac gagcaaaaat cgctgattga
      721 gtggtatcgc gatgtgttcc gtccggaaga tttacgtctg gttgaaagcg tacagaaagg
      781 gctgaaatcg cgtggctatc gtggtcaggg gcgcatcatg gccgacagta gcggtagtgg
      841 catttccgaa catggtatcg cccatttcca taatctgctg gcgcaggtgt ttaaggacta
      901 atgacatc
//
LOCUS       DAMZET010000401          906 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.406, whole genome shotgun sequence.
ACCESSION   DAMZET010000401 DAMZET010000000
VERSION     DAMZET010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.406"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>906
                     /locus_tag="POB34_004989"
     CDS             <1..>906
                     /locus_tag="POB34_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDI5921796.1"
                     /translation="SNTQVRSFTYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLN
                     PAGLSYTYQYEKDRITITDSLDRREVLHTQGEAGLKRVVKKEHADGSVTQSQFDAVGR
                     LKAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQLTSATGPDGLEMRRK
                     YDEYGRLIQETAPDGDITRYRYDNPHSDLPCATDDATGSRKTMTWSRYGQLLTFTDCS
                     GYVTRYDHDRFGQVTAVHREEGLSQYHAYDSRGQLTAVKDTQGHETRYEYNAAGDLTT
                     VIAPDGSRNGTQYDAWGKAVRTTQG"
BASE COUNT          229 a          261 c          277 g          139 t
ORIGIN      
        1 gcagcaatac acaggtgcgc agctttactt acgatgataa ataccggggc cggatggtgg
       61 cgcaccgtca cacgggccga ccggaaatcc gttaccgtta cgacagcgac gggcgggtga
      121 cagaacagct aaacccggca ggcttaagct acacgtatca gtatgagaaa gaccgcatca
      181 ccatcaccga cagcctggac cgccgtgaag tgctgcacac gcagggcgaa gccgggctga
      241 agcgggtggt gaaaaaggaa cacgcggacg gcagcgtcac gcagagtcag tttgatgcgg
      301 tgggcaggct taaggcacag acggatgccg caggcaggac aacagagtac agcccggatg
      361 tggtgacggg actcatcacg cgcatcacca cgccggatgg cagggcatcg gcgttttact
      421 ataaccacca cagccagtta acgtcagcca ccgggcctga cgggctggaa atgcgccgca
      481 aatatgatga gtacggacgt ctgattcagg aaaccgcccc tgacggcgat atcacccgct
      541 accgttatga taatccacac agtgacctcc cctgcgcaac ggacgatgcc accggcagcc
      601 ggaaaaccat gacgtggagc cgttacggtc agttgctgac cttcactgac tgttccggtt
      661 atgtaacccg ctatgaccat gaccgttttg gtcaggtgac ggcggtgcac cgcgaggaag
      721 gcctgagcca gtaccacgca tacgacagcc gtggacagtt aactgcggtg aaagacacgc
      781 agggccatga aacgcggtat gaatataacg ccgccggtga cctgaccacc gtcattgccc
      841 cggacggcag cagaaacggg acacagtacg atgcgtgggg aaaggccgtc cgtaccacgc
      901 agggcg
//
LOCUS       DAMZET010000402          887 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.407, whole genome shotgun sequence.
ACCESSION   DAMZET010000402 DAMZET010000000
VERSION     DAMZET010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.407"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..386)
                     /gene="nudJ"
                     /locus_tag="POB34_004990"
     CDS             complement(<1..386)
                     /gene="nudJ"
                     /locus_tag="POB34_004990"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HDI5921797.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIR"
     gene            complement(396..>887)
                     /gene="rluE"
                     /locus_tag="POB34_004991"
     CDS             complement(396..>887)
                     /gene="rluE"
                     /locus_tag="POB34_004991"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HDI5921798.1"
                     /translation="TDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARL
                     TQPGKRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPP
                     IRERKSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWRE
                     VTD"
BASE COUNT          198 a          243 c          224 g          222 t
ORIGIN      
        1 caacgaatac tttccgccac cagcggcgaa cgaagatttg acgcctgtaa aatttcttcg
       61 gcgctgaccc aacggcagca gtcgatatcg ctgtcatgag gctgggtcgg gcatatttgc
      121 tcaagctcaa tggcaaagag gaaacgcaaa aacggcgttt tatctggcgc aatccactga
      181 tgcatacgaa taaagtgttg cggctgcgcg ctgatgccgg tttcttccca cagttcacgg
      241 gcggcggctt ccactaaggt ttcatcggct tccagatgcc cggcaggttg gttccataac
      301 gctttaccat taatcgtctc ttcaacgact aaaaatttgc cttctgcgtg caccacgcaa
      361 gcaacggtaa cgtgcggttt aaacattttg catccttaat ctgtcacttc tcgccattcg
      421 ccattggcaa gattatccaa agagtaatct cccatcgcat agcgaatcag tcgcagcgtg
      481 gggaaaccaa catgggcggt catgcggcgc acctggcgat tacgtccttc atataaggtg
      541 atcttcagcc agctggtggg aatacttttg cgttcacgaa ttggtggatt ccgcggccat
      601 aaccacgcgg gttcgtcaac cagctccgcg cctgcgggca gggtagggcc atcatttaag
      661 gttacgccat tgcgcaaggc ttcaagtgcg tcttgtgtgg gaataccttc cacctgcaca
      721 taatagattt ttccggtgcg tttacccggc tgggttaaac gcgcctgcaa cgcgccgtta
      781 ttggtcagca ccagcaaccc ttcgctatcg cggtcaaggc gacctgctgc ataaacgccc
      841 tgaaccggga tgaattcttt taatgttttg cgtccggctt catcggt
//
LOCUS       DAMZET010000403          876 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.408, whole genome shotgun sequence.
ACCESSION   DAMZET010000403 DAMZET010000000
VERSION     DAMZET010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.408"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..214)
                     /locus_tag="POB34_004992"
     CDS             complement(<1..214)
                     /locus_tag="POB34_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HDI5921799.1"
                     /translation="MNRVTAIISALVICIIVYLSWAVNHYRDNAITYKAQRDKNAREL
                     KLANAAITDMQMRQRDVAALDAKYTKE"
     gene            complement(211..708)
                     /gene="rrrD"
                     /locus_tag="POB34_004993"
     CDS             complement(211..708)
                     /gene="rrrD"
                     /locus_tag="POB34_004993"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HDI5921800.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYVPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            complement(708..>876)
                     /gene="essD"
                     /locus_tag="POB34_004994"
     CDS             complement(708..>876)
                     /gene="essD"
                     /locus_tag="POB34_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HDI5921801.1"
                     /translation="AGSAGYWFLQLLDKVTPSQWAAIGVLGSLVFGLLTYLTNLYFKI
                     KEDKRKAARGE"
BASE COUNT          207 a          222 c          200 g          247 t
ORIGIN      
        1 actccttcgt gtattttgca tcgagcgcag caacatcacg ctgacgcatc tgcatgtcag
       61 taattgccgc gttcgccagc ttcagttctc tggcattttt gtcgcgctgg gctttgtagg
      121 taatggcgtt atcacggtaa tgattaacag cccatgacag gtagacgatg atgcagataa
      181 ccagagcgga gataatcgcg gttactctgt tcattgctga ccccacaaac agatttcacg
      241 ctcaatctca cgacgagtca tgagaccttt ccattgctta ccgccagcat atgtccagcg
      301 acgtagctga tcacatgcgc ctttgatatc gccctggttt attttgcgaa gaagcgtcga
      361 tgttctgaaa ttgccagcac ccacgttgta aacgaatgag taaagagcgc cgcgcgttgt
      421 ttccggtata tcgactttga tgtacgggtt aatttgtctg gcgacagtgg caaggtcttt
      481 attcaagagt gctttgcatt ctgctttggt atacgtttta ccgagcatga tgtcttttcc
      541 ggtgtgtccg tgacatacag tccatacgcc aacgatatct ttgtatggta cgtagctgac
      601 accttccaga ccatcgttac cacttgggcc agtaattaac actgatgcta tagcaattgc
      661 tccgccacca atagcagcag caacggcttt tcgtaatgat ggaggcatta ttcacctctc
      721 gcagccttgc gcttatcttc tttaatcttg aaataaaggt ttgtcaggta cgtcagcagg
      781 ccaaatacca ggctacccag cacacctatt gctgcccact gtgagggcgt gactttatcg
      841 agcagctgta aaaaccagta accggcacta cctgct
//
LOCUS       DAMZET010000404          866 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.409, whole genome shotgun sequence.
ACCESSION   DAMZET010000404 DAMZET010000000
VERSION     DAMZET010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.409"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>866
                     /locus_tag="POB34_004995"
     rRNA            <1..>866
                     /locus_tag="POB34_004995"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          220 a          195 c          285 g          166 t
ORIGIN      
        1 gagtaatgtc tgggaaactg cctgatggag ggggataact actggaaacg gtagctaata
       61 ccgcataacg tcgcaagacc aaagaggggg accttcgggc ctcttgccat cggatgtgcc
      121 cagatgggat tagctagtag gtggggtaac ggctcaccta ggcgacgatc cctagctggt
      181 ctgagaggat gaccagccac actggaactg agacacggtc cagactccta cgggaggcag
      241 cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg tgtatgaaga
      301 aggccttcgg gttgtaaagt actttcagcg gggaggaagg gagtaaagtt aatacctttg
      361 ctcattgacg ttacccgcag aagaagcacc ggctaactcc gtgccagcag ccgcggtaat
      421 acggagggtg caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag gcggtttgtt
      481 aagtcagatg tgaaatcccc gggctcaacc tgggaactgc atctgatact ggcaagcttg
      541 agtctcgtag aggggggtag aattccaggt gtagcggtga aatgcgtaga gatctggagg
      601 aataccggtg gcgaaggcgg ccccctggac gaagactgac gctcaggtgc gaaagcgtgg
      661 ggagcaaaca ggattagata ccctggtagt ccacgccgta aacgatgtcg acttggaggt
      721 tgtgcccttg aggcgtggct tccggagcta acgcgttaag tcgaccgcct ggggagtacg
      781 gccgcaaggt taaaactcaa atgaattgac gggggcccgc acaagcggtg gagcatgtgg
      841 tttaattcga tgcaacgcga agaacc
//
LOCUS       DAMZET010000405          859 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.410, whole genome shotgun sequence.
ACCESSION   DAMZET010000405 DAMZET010000000
VERSION     DAMZET010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.410"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..283
                     /locus_tag="POB34_004996"
     CDS             <1..283
                     /locus_tag="POB34_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HDI5921802.1"
                     /translation="DRLWPRGIKKTDLALDEWDKEITPSTELRKAFHGEVVDFATFRE
                     QYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQNHALVLADWLRSL"
     gene            complement(305..559)
                     /gene="yoaF"
                     /locus_tag="POB34_004997"
     CDS             complement(305..559)
                     /gene="yoaF"
                     /locus_tag="POB34_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HDI5921803.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            742..>859
                     /locus_tag="POB34_004998"
     CDS             742..>859
                     /locus_tag="POB34_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDI5921804.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMEST"
BASE COUNT          222 a          224 c          195 g          218 t
ORIGIN      
        1 cgaccgcctc tggccgcgcg gtatcaaaaa aaccgattta gcccttgatg agtgggataa
       61 agaaatcacg ccgtcaacgg aactgcgcaa agcctttcac ggcgaagtcg tcgattttgc
      121 aacctttcgc gagcaatatc ttgcagaact ggcacaacac gagcaagaag gaaagcggct
      181 ggcggacatc gccaaaaaac agccgctgac cctgctctac gcagcaaaaa acaccacgca
      241 gaaccatgcg ctggtgctgg ccgactggct acgtagcttg tgattttagt acagcatccg
      301 gcggttattt ttcaccagcc ggatggtcac gccgccacaa tgcccattca tcaatcgttt
      361 caccgcccgg taatttgcaa ttgttgctga ccccttgcgc tgtctgcact ggaatgagcg
      421 tcccgccctt ctgctggcaa tagaccgacg ccggatttgc cataccaatc tgcggcggtt
      481 taggtgcttc tggctgagaa ggggttgaac aaccagccag gaccagcaag caaggcagaa
      541 caaaactgat aattttcatt tattgatctc acatatttat ccaagattag agtatcgcgg
      601 tatcgttttg ttttgcagca ctatttttat tacattcact caaaacatat tacgtcttgt
      661 ttcatctttg ttgatgatgt tttatcatgc ctgcaaagat taaataatca gcatttaccc
      721 gccgtatcct ggagttgttc cgtgtcagat cagattatcg cccgcgtctc gcaatccctt
      781 gccaaagaac agtcactgga aagtctggtc cgacaacttc tggagatgct ggaaatggtc
      841 actgatatgg aatcaacct
//
LOCUS       DAMZET010000406          848 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.411, whole genome shotgun sequence.
ACCESSION   DAMZET010000406 DAMZET010000000
VERSION     DAMZET010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.411"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            115..462
                     /locus_tag="POB34_004999"
     CDS             115..462
                     /locus_tag="POB34_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921805.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGVLSGLCMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(482..>848)
                     /locus_tag="POB34_005000"
     CDS             complement(482..>848)
                     /locus_tag="POB34_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921806.1"
                     /translation="VELTDAGRAVAQPLLLAEQDRLRAEQRAAEVVVLPLVPAAGLPA
                     DGTSATDLAVQLNGITYQACRGDFVVRLDGSTCLQLWNKEGRVVRREGDPLEVAQWLQ
                     ACHDAGMEVRVQINESAAP"
BASE COUNT          168 a          239 c          244 g          197 t
ORIGIN      
        1 gtattgcata ctgcacagaa gtggccatag aggggctgtc cggatatcac agcatgataa
       61 taatcaataa caataagctg tgtcacgttt acatcatgga acggaggtgc tcctgtgacc
      121 tttgagcaga aaaaagcccg ggccattgcc ctgatggaca gcaaaaagat gtggcggagc
      181 aattatgccc ctcctcttct gcgtattctc tggcggctgg gtatccggct tccgccatta
      241 ccctttatgc cgttctggca ggtcacggtg ctgacggggg gcttatgggg catttcctgg
      301 ggatgtgcaa tgtggtttat ctactggggg ccgtcaggaa tggtggcagg tgaggccatt
      361 attataagta tcacgggggg ggttttgtcc ggactgtgca tggcctcttt ccactggtgg
      421 cgcaggaaag ttaaccggct gccaccatgg gatgatgtgt aaaccgtgca caggcgtgtt
      481 cttacggagc ggcactttca tttatctgta cgcgaacctc cattcctgca tcgtgacagg
      541 cctgcagcca ctgcgccact tccagcggat cgccctcccg gcgtaccacc ctgccttctt
      601 tattccataa ctgcagacag gtgctgccgt ctagacgcac cacaaaatcc ccacggcagg
      661 cctgatacgt tatgccgttc agttgcaccg ccagatcagt ggcagaagtg ccatctgcgg
      721 gcagacctgc tgccgggacc agcggcagta cgacgacttc tgccgcacgc tgttctgccc
      781 ggaggcggtc ctgttctgcc agcagcagag gctgtgccac ggcccggccg gcgtccgtca
      841 gctctact
//
LOCUS       DAMZET010000407          782 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.413, whole genome shotgun sequence.
ACCESSION   DAMZET010000407 DAMZET010000000
VERSION     DAMZET010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.413"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
BASE COUNT          221 a          190 c          148 g          223 t
ORIGIN      
        1 gcgctcctaa aattcaattc acaacgcaga cctacaacat agctaaaaac actcgtaatt
       61 taaggctcgg ggtccatgca tattgctcat ggacttacct gaatggctca ccctttggtg
      121 gttttcaaca ggtatattcc gaccaaaaca acgtttggta tgtgagtaat tatgcttggg
      181 gaaattatga gtctggtggg accatatcag tcacatgcct caatcttcct ggtgctggag
      241 cttaattaaa ggggcaacag taggcatcga catgttcctc atcaacacca tcaccaatac
      301 cgtacatgcg tgttccagtc ataatgtatc cagtcggaca ttgaatcggc ttgtaataga
      361 tcgaaccacc actcttaccg ccgataaagt gattcattgc tacggatgat ttgtaccaat
      421 tacatgctga gtaattaatt ttattacccc ctgaccaccg accggattgg cacgtaggcc
      481 agcagcgcca gcaatttaat ctcagttctg acacgcgacg acataactac attttccggt
      541 taaatcagct ctaaatgtcc ctttatggaa attgtattca tcataaaaac cacatttaac
      601 aattcgattt gcatcataag ttgcaactga tcctgcaaag taattcggct tccaaccttg
      661 agggttttct gcattgctca cactccatga gcactgactg gcagaaaatc ctgttggcag
      721 cggtatttgc tgaccatttg ctatacgacc agttatcacc gtactaccag aattgctttt
      781 ac
//
LOCUS       DAMZET010000408          757 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.414, whole genome shotgun sequence.
ACCESSION   DAMZET010000408 DAMZET010000000
VERSION     DAMZET010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.414"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..486
                     /gene="mlc"
                     /locus_tag="POB34_005001"
                     /gene_synonym="dgsA"
     CDS             <1..486
                     /gene="mlc"
                     /locus_tag="POB34_005001"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HDI5921807.1"
                     /translation="GHTQVDPYGKRCYCGNHGCLETIASVDSILELAQLRLNQSMSSM
                     LHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFNPQKILIGSPLSKA
                     ADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKDAMYNGSLLIRLLQ
                     G"
     gene            611..>757
                     /locus_tag="POB34_005002"
     CDS             611..>757
                     /locus_tag="POB34_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI5921808.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPE"
BASE COUNT          191 a          173 c          196 g          197 t
ORIGIN      
        1 ggccacacac aggtcgaccc gtatgggaaa cgttgttatt gcgggaatca cggctgcctc
       61 gaaaccatcg ccagtgtgga cagtatactt gagctggcac agctgcgtct caatcaatcc
      121 atgagctcga tgttacatgg gcaaccgtta accgtggact cattgtgtca ggcggcattg
      181 cgcggcgatc tgctggcaaa agacatcatt accggggtgg gcgcgcatgt ggggcgcatt
      241 cttgccatca tggtgaattt atttaaccca caaaaaatac tgattggctc accgttaagt
      301 aaagcggcag atatcctctt cccggtcatc tcagacagca tccgtcagca ggcccttcct
      361 gcgtatagtc agcacattag cgttgagagt actcagtttt ctaaccaggg cacgatggca
      421 ggcgctgcgc tggtaaaaga cgcgatgtat aacggttctt tgttgattcg tctgttgcag
      481 ggttaacatt ttttaactgt tctaccaaaa tttgcgctat ctcaatttgg gccaggaaag
      541 cataacttag actttcaagg ttaattattt tcatggttta tatttgtgaa gcataacggt
      601 ggagttagtg atgctgaagc gtttctttat taccggtaca gacacttctg tagggaaaac
      661 ggtggtttcc cgcgcattgc tacaagcgtt agcctcccag ggaaaaacgg ttgcgggata
      721 taaacccgta gcgaagggga gcaaagagac acccgaa
//
LOCUS       DAMZET010000409          756 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.415, whole genome shotgun sequence.
ACCESSION   DAMZET010000409 DAMZET010000000
VERSION     DAMZET010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.415"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..450
                     /gene="pspF"
                     /locus_tag="POB34_005003"
     CDS             <1..450
                     /gene="pspF"
                     /locus_tag="POB34_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707212.3"
                     /note="transcriptional activator for pspABCE which are
                     induced in response to phage proteins, membrane altering
                     stresses, and impaired proton expor; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HDI5921809.1"
                     /translation="PPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTEHARETLLNY
                     RWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLPLD
                     LREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(581..>756)
                     /locus_tag="POB34_005004"
     CDS             complement(581..>756)
                     /locus_tag="POB34_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDI5921810.1"
                     /translation="EPSAAIAQKIQQRALAQGLLLLTCGAYGNVIRFLYPLTIPDAQF
                     DAAMKILQDALRD"
BASE COUNT          192 a          194 c          188 g          182 t
ORIGIN      
        1 ccgccactac gcgaacgtga aagcgacatt atgttaatgg cggaacactt tgccatccag
       61 atgtgtcggg aaatcaagct gcctctgttc ccgggtttta ccgagcacgc cagagaaaca
      121 ttgctgaatt atcgctggcc gggaaatatt cgtgaattga aaaacgtggt ggaacgttca
      181 gtgtatcgcc acggcaccag cgattatccg cttgatgaca tcattattga tccctttaaa
      241 cggcgtccac ctgaagaagc catcgccgtt tcagaaaaca cttcgcttcc aacactgccg
      301 ctggatttac gtgagtttca gatgcagcag gaaaaagagt tgctgcaact cagtttgcaa
      361 caagggaaat ataaccagaa gcgcgcagct gaattgctgg gcttaaccta tcatcagttc
      421 cgcgcgttgt tgaaaaagca ccagatttag cgaagttggg caatacaact tgccggatgc
      481 ggcgtgaacg ccttatccgg catacataat caatacgtcg tttgcaggtc tgataagcgc
      541 agcatatcag gcattattgc gttgcccctg cttctgcgta ttaatctctc agcgcatcct
      601 gcaaaatttt cattgccgca tcgaattgcg catccgggat ggtcagcgga tacaggaaac
      661 gaatcacgtt gccgtatgcg ccacaggtca gtagaagcag tccctgcgcc agcgcgcgtt
      721 gctggatttt ctgtgcaatc gccgctgacg gctcgc
//
LOCUS       DAMZET010000410          733 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.416, whole genome shotgun sequence.
ACCESSION   DAMZET010000410 DAMZET010000000
VERSION     DAMZET010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.416"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>733
                     /gene="rarA"
                     /locus_tag="POB34_005005"
                     /gene_synonym="mgsA"
     CDS             <1..>733
                     /gene="rarA"
                     /locus_tag="POB34_005005"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HDI5921811.1"
                     /translation="GATTENPSFELNSALLSRARVYLLKSLSTEDIEQVLTQAMEDKT
                     RGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMMADMAEVDDSGKRVLKPELLTEI
                     AGERSARFDNKGDRFYDLISALHKSVRGSAPDAALYWYARIITAGGDPLYVARRCLAI
                     ASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIAQAIVYLACAPKSNAVYTAFKAA
                     LADARERPDYDVPVHLRNAPTKLMK"
BASE COUNT          165 a          184 c          215 g          169 t
ORIGIN      
        1 ttggcgcaac cactgaaaac ccgtcgtttg agcttaattc ggcactgctt tcccgtgccc
       61 gtgtctatct attgaaatcc ctgagtacag aggatattga gcaagtacta actcaggcga
      121 tggaagacaa aacccgtggc tatggtggtc aggatattgt tctgccagat gaaacacgac
      181 gcgccattgc tgaactggtg aatggcgacg cgcgccgggc gttaaatacg ctggaaatga
      241 tggcggatat ggccgaagtc gatgatagcg gtaagcgggt cctgaagcct gaattactga
      301 ccgaaatcgc aggtgaacgt agcgcccgct ttgataacaa aggcgatcgc ttttacgatc
      361 tgatttccgc actgcataag tcggtacgtg gtagcgcacc cgatgcggcg ctctactggt
      421 atgcgcgaat tattaccgcc ggtggcgatc cgttatatgt cgcgcgtcgt tgcctggcaa
      481 ttgcttccga agatgtcggc aatgccgacc cacgggcgat gcaggtggca attgcggcct
      541 gggattgctt tactcgcgtt ggcccggcgg aaggtgaacg cgccattgct caggcgattg
      601 tttacctagc ctgcgcgcca aaaagcaacg ctgtctacac tgcgtttaaa gccgcgctgg
      661 cggatgctcg cgaacgtccg gattatgacg tgccggttca tttgcgtaat gcgccgacga
      721 aattaatgaa gga
//
LOCUS       DAMZET010000411          719 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.417, whole genome shotgun sequence.
ACCESSION   DAMZET010000411 DAMZET010000000
VERSION     DAMZET010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.417"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            32..>719
                     /gene="manC"
                     /locus_tag="POB34_005006"
     CDS             32..>719
                     /gene="manC"
                     /locus_tag="POB34_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase ManC"
                     /protein_id="HDI5921812.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACE"
BASE COUNT          164 a          187 c          206 g          162 t
ORIGIN      
        1 gtcaatttat aaatgatatt cggggataat tatggcgcag tcgaaactct atccagttgt
       61 gatggcaggt ggctccggta gccgcttatg gccgctttcc cgcgtacttt atcccaagca
      121 gtttttatgc ctgaaaggcg atctcaccat gctgcaaacc accatctgcc gcctgaacgg
      181 cgtggagtgc gaaagcccgg tggtgatttg caatgagcag caccgcttta ttgtcgcgga
      241 acagctgcgc caactgaaca aactcaccga gaacattatt ctggaacctg cagggcgtaa
      301 cactgcacct gccattgcgc tggcggcgct ggcggcaaaa cgtcatagcc cggagagcga
      361 cccgttaatg ctggtattgg cggcggatca tgtgattgcc gatgaagacg cgttccgtgc
      421 cgccgtgcgt aatgccatgc cgtatgccga agcgggcaag ctggtgacct tcggcattgt
      481 gccggatcta cctgaaaccg gatatggcta tattcgtcgc ggtgaagtat ctgcgggtga
      541 gcaggatacg gtggcctttg aagtggcgca gtttgtcgaa aaaccgaatc tggaaaccgc
      601 ccaggcctat gtggcaagcg gcgaatatta ctggaacagc ggcatgttcc tgttccgcgc
      661 cggacgctat ctcgaagaac tgaaaaaata tcgcccggat attctcgacg cctgtgaac
//
LOCUS       DAMZET010000412          691 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.418, whole genome shotgun sequence.
ACCESSION   DAMZET010000412 DAMZET010000000
VERSION     DAMZET010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.418"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(162..691)
                     /locus_tag="POB34_005007"
                     /pseudo
     CDS             complement(162..691)
                     /locus_tag="POB34_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          134 a          206 c          180 g          171 t
ORIGIN      
        1 gctagtcccg gattattcaa tattagagag tcaaatattc tttgtgatgc tgtatcgttc
       61 caccattttg tcaatgcttt tgcagcatta tcatctgctt gctgctgtgc agggcttttg
      121 ggccatggaa ttggcaaggg gaatggtcta ggaaaaactt ctaaccccag aggatctgta
      181 ttcgtaactg gattcaacgg atactgataa aaattccatc cccccttcag cccaatcgga
      241 tcctgagtga tatacctccc ccgcagcggg tcataatagc ggtggcggtt ataatacagg
      301 ccggactcct catcatactg ctgccccggc aggcggatga gctgctgcag ctggtgcggg
      361 ttctcttcat tcagctggtt accccattca tcatattctg cgcaccacgc tgttgcccct
      421 tccgtgctga tgagcgccag cggcaggccg cgatggtcgc agtggtacag gtggattttt
      481 cgcgccggcg tgtacaccgg gtccatctgg ttttgcatct gctccacggt caggccgcac
      541 gatgccagcc agcggcggct ttcctcactc acccggtcag ccaggatttc actttccagc
      601 cggtcgagca tctgcaccag caccggcggg aacaccacac tgccaccgtc ttcgccgccg
      661 gactgctgaa gcgcatccgc caggctgcgg c
//
LOCUS       DAMZET010000413          691 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.419, whole genome shotgun sequence.
ACCESSION   DAMZET010000413 DAMZET010000000
VERSION     DAMZET010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.419"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..606
                     /locus_tag="POB34_005008"
     CDS             <1..606
                     /locus_tag="POB34_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HDI5921813.1"
                     /translation="LGNTELSFAAKVWGSSDRQNVFEVGTSAAYLFYAQKTSAGQLFD
                     VNGAINCTTLNQSSDRDLKDDILVISDATKAIRKMNGYTYTLRENGMPYAGVIAQEVM
                     EAIPEAVGSFTHYGEELQGPTVDGNELREETRYLNVDYAAVTGLLVQVARETDGRVTA
                     LEEENITLRKNLATAGTRISTLENQVSELVALVGQLTGSEH"
BASE COUNT          202 a          144 c          186 g          159 t
ORIGIN      
        1 ttaggtaata cagaactatc atttgccgca aaagtatggg gatcaagtga tcgacaaaac
       61 gtttttgagg ttggaacgtc tgctgcgtat ctgttttatg cgcaaaaaac gtcagcaggc
      121 cagttgtttg atgtaaatgg cgctattaat tgcacaacgc tgaatcagtc atcagaccgc
      181 gaccttaaag acgatattct cgttatcagc gacgcgacga aagcaatccg taaaatgaac
      241 ggatacacct acacgctcag ggaaaacggg atgccttatg ctggcgttat tgcacaggaa
      301 gtaatggagg cgataccaga agctgtggga tcgtttactc attacggtga agagttgcag
      361 ggaccaacgg ttgacggcaa tgaattacgt gaagaaacac gttatctgaa tgttgactac
      421 gccgccgtga cggggttact tgttcaggtc gcccgtgaaa cagatggtcg cgttaccgcg
      481 ctggaagagg aaaacataac gctacgtaaa aatctggcaa cagcaggcac ccggatcagc
      541 actctggaaa atcaggtaag cgaactggtt gcacttgtcg ggcagttaac aggaagcgaa
      601 cattgatatc cttcaagccc tgaaggaggc tgttcctggt acgttcagac tgttgttgag
      661 ctggaaatcg caacggagga agaaacttcg t
//
LOCUS       DAMZET010000414          685 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.420, whole genome shotgun sequence.
ACCESSION   DAMZET010000414 DAMZET010000000
VERSION     DAMZET010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.420"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..348)
                     /locus_tag="POB34_005009"
     CDS             complement(<1..348)
                     /locus_tag="POB34_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HDI5921814.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASF"
     gene            complement(353..>685)
                     /locus_tag="POB34_005010"
     CDS             complement(353..>685)
                     /locus_tag="POB34_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDI5921815.1"
                     /translation="FPYASGEKGYSNWELSADRANASRRELMVGGLDSGKVLRVVGMA
                     ATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHENAESQNEPVSALEKPEVAPQVSVP
                     TMPSAEPR"
BASE COUNT          139 a          190 c          172 g          184 t
ORIGIN      
        1 gaagctggcg gcatccggct cttgcgactg tttataagcg tcgagctgtt cttgcatgat
       61 gtccttcgtt tccaaaaaca gattgataat gtcggtgttg agttgcatct cacctcgtct
      121 ggcttcatcg agcaggtttt ccatcagatg cgtggtttcc tgcaaaacgc tgaagccaaa
      181 agttcctgcc cctcctttga tcgagtgggc agcccgaaag atggcattca attgttcggc
      241 atctggcgct tccggctgca aaaccagcag atgctgctcc atgtcagcca acagttcgtc
      301 cgcttcatca aaaaatgtct gataaaaatc gcttatatcc atgctcacgc tgtcacctcg
      361 gttcggctga tggcattgtg ggaacactga cctgtggtgc aacctcaggt ttttccaggg
      421 cgcttactgg ctcattctgg ctttcggcgt tttcatgcaa aatggcctgt tcggcttgtt
      481 tgttcagtac cagcaggctg atgcgacggt tgacggcatc atcaggtccg cgatcgctta
      541 agcgcatcgt tgccgccatg ccgacaacac gtaacacttt gccgctatcc aaccctccga
      601 ccatcagttc gcggcgggat gcattggccc gatcggcaga aagctcccag ttgctatatc
      661 ctttctcacc gctggcgtag gggaa
//
LOCUS       DAMZET010000415          678 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.421, whole genome shotgun sequence.
ACCESSION   DAMZET010000415 DAMZET010000000
VERSION     DAMZET010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.421"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..>678)
                     /locus_tag="POB34_005011"
     CDS             complement(<1..>678)
                     /locus_tag="POB34_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDI5921816.1"
                     /translation="VTNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVE
                     EIMARLAKEVRQAFSGVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPR
                     FNSRIKRQSEQWQLVLLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDL
                     TADVADPVELLMKVQLGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTH
                     QVKKCVQ"
BASE COUNT          159 a          183 c          174 g          162 t
ORIGIN      
        1 ctctggacac actttttcac ctgatgcgtc agtaatgatg atgaaccccc ttcgtaaaaa
       61 tcgctcacga ggataatgac gcttttcgct ggttgttcaa taagttgccg accatactcc
      121 acggcactgg cgatattggt cccgccgccc aactgtactt tcattaataa ctctaccgga
      181 tcggcaacgt ctgccgtgag atcaacgacg cttgtgtcaa acgccaccag atgggtacga
      241 atgccgggta actgccacaa acaggccgcc atcaccgcag agtggatcac cgagtccacc
      301 atcgatccgc tttgatcaac cagtaagacc agttgccatt gttcgctttg gcgtttaatg
      361 cggctgttaa agcgggggga ttcgatatac aacttgccgt gttgcgggtg ccagtgttgc
      421 aggttggcgc gcagagtact tttgaaatca aagtttcgcg ccagtggaat taatgagcgg
      481 cgacggcgat cgcggacacc agaaaaagcc tgacgaactt cctttgccag tcgcgccata
      541 atttcttcaa caacctggcg cactatctgg cgggcggcag ccagtacttc gggattcatc
      601 agatgtttgg tgtgcaaaac ggcgcgtagc aggctttccg aaggctgcat acgttccagc
      661 acgtcgagat ttgtcacc
//
LOCUS       DAMZET010000416          651 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.422, whole genome shotgun sequence.
ACCESSION   DAMZET010000416 DAMZET010000000
VERSION     DAMZET010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.422"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(1..651)
                     /locus_tag="POB34_005012"
                     /pseudo
     CDS             complement(1..651)
                     /locus_tag="POB34_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
BASE COUNT          167 a          172 c          147 g          165 t
ORIGIN      
        1 gccatgaaat acattaaaat ccaccggacc attgatgcca tctacctgtc cacggtcgga
       61 atgctgccag aagcgccagg ccatgccgtc attgtccgga cgacgttgat aatagtgagc
      121 cacccaccac ggatattcat tgaaatagcc cgccagattg gtgtgataaa aaacggctcc
      181 tgagtaaata atcggctttt ttcccgtact tttttcgacc atttttagcc actgacttac
      241 tcgtttgcgt aattcttttg ccgataattt tccccgttct tctacgtcca gtaccgcagg
      301 gaaatcgcct tgtgagaaat ccaccgtttg cagaaataat cgtgcctgaa ccggcgctgc
      361 taccgacggg gagaaataat gatacgcccc gcgcagcagg ccattttcgc ggcttagttg
      421 ccagttacgc gaaaaatacg ggtccaccag cttttcgcct tccgtcgcct taataaataa
      481 aagcaaactg taagcggata ccgttgtcgc gcatttttgc cacccgctgc cagtcgatcc
      541 gctcctgcca gcgagaaaca tctattccgt gaatggtgta actggcagga atgcgaatag
      601 cgaaagattt aaccgggcga taaccgtaga aatggatgta gtctgtaatc t
//
LOCUS       DAMZET010000417          615 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.423, whole genome shotgun sequence.
ACCESSION   DAMZET010000417 DAMZET010000000
VERSION     DAMZET010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.423"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..292)
                     /locus_tag="POB34_005013"
     CDS             complement(<1..292)
                     /locus_tag="POB34_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDI5921817.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIR"
     gene            complement(322..>615)
                     /gene="kduI"
                     /locus_tag="POB34_005014"
     CDS             complement(322..>615)
                     /gene="kduI"
                     /locus_tag="POB34_005014"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDI5921818.1"
                     /translation="TELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFHMMGQPQE
                     TRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKDLR"
BASE COUNT          162 a          181 c          134 g          138 t
ORIGIN      
        1 ggcgaatcaa tccggcgtta ttcaccagga tatcaatatg accaaactcc gctaccgcgc
       61 gatccagcag tgctggaata ccatcaatct ttcgcagatc ggcggtcagg cttaaaaaac
      121 gacgccccag cgctgtgacc tgcttgatgg tttcagtcgg ttcaacgatg ttaatgccaa
      181 caatgtcaca gcccgcttgc gccagcccca acgccatccc ctggcccagt ccagtatcac
      241 aaccagtgac gaccgcaact ttaccttcga gagaaaatgc acttaaaatc ataacaatac
      301 cttattcgtt tatgccgaca actagcgcaa atctttaacg gccacatggt ccatatcatc
      361 aaagacctgg ttttcaccga ccatgcccca gataaaggta taagctttgg ttccgacgcc
      421 ggaatgaatc gaccagctcg gggagatcac cgcctgctcg ttatgcatca caatgtgacg
      481 cgtttcttgc ggctgcccca tcatgtggaa aacgcaggcg tcatcatcca tattgaaata
      541 gaaataaact tccatccggc gctcgtgggt gtgacacggc atggtgttcc acaagttacc
      601 cggagccagc tccgt
//
LOCUS       DAMZET010000418          610 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.424, whole genome shotgun sequence.
ACCESSION   DAMZET010000418 DAMZET010000000
VERSION     DAMZET010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.424"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..>610)
                     /locus_tag="POB34_005015"
     CDS             complement(<1..>610)
                     /locus_tag="POB34_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDI5921819.1"
                     /translation="AGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMA
                     AWKLAPALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTG
                     HPKVRMVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTF
                     GYYNAGQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPD"
BASE COUNT          145 a          194 c          139 g          132 t
ORIGIN      
        1 tcatccggcg caccagattt taacgttgcc accgcagcac ccagtttttc caccagcgta
       61 tcgtaaatgc ctttttgcgc gtagatccga caagccgcag tacaatcctg tccagcattg
      121 taatagccaa atgtacgtac accttcgacc actgcttcaa tatccgcatc atcaaaaaca
      181 atcactggcg ctttgccacc aagttccata tgagtacgct taatggacga cgcggtatgg
      241 ctgatgatgt gctcgccggt ggcgatagag cccgtcagcg acaccatccg cactttggga
      301 tgaccggtca gcggatcacc caccgttttg cctctgccaa acagtacgtt aatcacgcct
      361 gccgggaaga tatctttcgc cagctctgcc aacttcaacg cggtcagcgg ggtaatttct
      421 gatggtttaa gcactacgca gttccctgcc gccagcgccg gagccagttt ccacgctgcc
      481 atcatcagcg gataattcca cggtgcgata gaagccacga cccccaacgg atcgcgacgg
      541 atcatcgaag tatgaccttc aagatattca cctgccgcca gaccattcag acagcgcgcc
      601 gcacccgcga
//
LOCUS       DAMZET010000419          605 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.425, whole genome shotgun sequence.
ACCESSION   DAMZET010000419 DAMZET010000000
VERSION     DAMZET010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.425"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..>605)
                     /gene="yeeE"
                     /locus_tag="POB34_005016"
     CDS             complement(<1..>605)
                     /gene="yeeE"
                     /locus_tag="POB34_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HDI5921820.1"
                     /translation="RGRFCLTGGFRDMYLAKNNRMFYALLIAISVQSVGVFALIQAGL
                     LTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRAGEGLIGSWIALFTYMVMSAV
                     MRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVLLVITLWVVMKELKKPKLKVA
                     TLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEAT"
BASE COUNT          182 a          176 c          129 g          118 t
ORIGIN      
        1 ggttgcttca ctcaggggcc aggctaaaag cgcaatcaaa ccgatgagta cagcagtgac
       61 aaagggatgc cagcgttttt caaacagaat atgagcgatc ccggttcggc gcggcggtaa
      121 ggtcgcgact ttgagttttg gcttcttcaa ctctttcatc accacccaga gcgttatcac
      181 cagcaaaacg gcaaccaacg gccacacaga caaattaaaa gtctcagcaa tagagttatg
      241 ttcagtactg tagtgctgca aggtttgatt taaaccactg gcatgtggag aacgcatcac
      301 cgcactcatc accatataag tgaaaagcgc gatccaactg ccgatcaatc cctcacccgc
      361 gcgataccag gtcccggtgg cacatccacc cgccagaaca attcccagcc caaagatata
      421 cccacctata acagtaccaa gccacgggaa cgccccggct tcgtaagtca gtaggcccgc
      481 ctgaattaac gcaaaaaccc ccacactttg taccgaaatt gcaatcagca aggcgtaaaa
      541 catgcgatta tttttcgcga gatacatatc gcgaaaacca cctgtcaggc aaaaacgccc
      601 acgct
//
LOCUS       DAMZET010000420          590 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.426, whole genome shotgun sequence.
ACCESSION   DAMZET010000420 DAMZET010000000
VERSION     DAMZET010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.426"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>590
                     /locus_tag="POB34_005017"
     CDS             <1..>590
                     /locus_tag="POB34_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921821.1"
                     /translation="ILNGWNSPQAQLAEQALRSGHIEALINIWENDNFSRYRPEKSVW
                     NLYLLAQLPREMALTFWLRIIEKKHLFAGEDYFLSILGLDALPGLLLAFSHRPKETFP
                     LILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPLVFTKPSDNSEAA
                     LLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLL"
BASE COUNT          140 a          146 c          150 g          154 t
ORIGIN      
        1 gatcctgaat ggctggaact ccccgcaagc acaattagca gaacaggcgc tccgcagcgg
       61 tcacatagaa gcgttaatta acatatggga aaatgacaac ttctcacgtt atcgtccgga
      121 aaagagtgtc tggaacctgt atttattggc acagttgccg cgtgagatgg ctttgacctt
      181 ctggctgcgt atcattgaga aaaagcatct gttcgcgggt gaggactatt ttctcagtat
      241 cctcggattg gatgcgctac caggtctgct gttggctttt tcacatcgtc caaaagaaac
      301 atttccgtta attttaaatt ttggcgcaac agaactggcg ctgcctgttg cccgcgtctg
      361 gcgacgtttc gcggcgcagc gtgatctggc tcgccagtgg attttacaat ggccggaaca
      421 tacggctagt gcacttatcc ctcttgtctt taccaaaccc agcgataata gcgaagccgc
      481 attacttgcc ctgcgtttac tgtacgaaca gggacatggc gaattgctac aaaccgtggc
      541 aaaccgctgg cagcgtacag atgtatggtc tgccctggag cagttgctta
//
LOCUS       DAMZET010000421          564 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.428, whole genome shotgun sequence.
ACCESSION   DAMZET010000421 DAMZET010000000
VERSION     DAMZET010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.428"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..515
                     /locus_tag="POB34_005018"
     CDS             <1..515
                     /locus_tag="POB34_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HDI5921822.1"
                     /translation="EPEPEPEPIPEPPKEAPAVIEKPKPKPKPKPKPVKKVQEQPKRD
                     VKPVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPRALSRNQPQYPARAQALR
                     IEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPGKPGSGIVVNIL
                     FKINGTTEIQ"
BASE COUNT          171 a          148 c          150 g           95 t
ORIGIN      
        1 tagagccaga accggaacct gagccgatcc ccgaaccacc aaaagaagca ccggcggtca
       61 ttgaaaagcc gaagccgaaa cctaagccaa aaccgaagcc ggtgaaaaag gtacaggagc
      121 agccaaaacg cgatgtcaaa cccgtagagt cgcgtccggc atcaccgttt gaaaatacgg
      181 cacccgcacg cctgacatca agtacggcaa cggctgcaac cagcaagccg gttaccagtg
      241 tggcttcagg accacgcgca ttaagccgta atcagccgca gtatccggca cgagcacagg
      301 cattgcgcat tgaagggcag gttaaagtta aatttgacgt cacgccggat ggtcgcgtgg
      361 ataacgtaca aatcctctca gccaagcctg cgaacatgtt tgagcgtgag gtgaaaaatg
      421 cgatgcgcag atggcgttat gagccgggta agccaggcag tgggattgtg gtgaatatcc
      481 tgtttaaaat taacggcacc accgaaattc agtaagcaga aagtcaaaag cctccgaccg
      541 gaggcttttg actattactc aaca
//
LOCUS       DAMZET010000422          560 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.429, whole genome shotgun sequence.
ACCESSION   DAMZET010000422 DAMZET010000000
VERSION     DAMZET010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.429"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..459
                     /locus_tag="POB34_005019"
     CDS             <1..459
                     /locus_tag="POB34_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HDI5921823.1"
                     /translation="YSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYR
                     GLVKIMPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQL
                     FYCLQRGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            469..>560
                     /gene="sufC"
                     /locus_tag="POB34_005020"
     CDS             469..>560
                     /gene="sufC"
                     /locus_tag="POB34_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="part of SUF system involved in inserting
                     iron-sulfur clusters into proteins; in Escherichia coli
                     this protein forms a complex with SufBD; the SufBCD
                     complex stimulates the cysteine desulfurase SufS in
                     conjunction with SufE; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDI5921824.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHA"
BASE COUNT          160 a          141 c          136 g          123 t
ORIGIN      
        1 tactcagtgg cgctgaccag cggtcatcag caagcggata ccggcaccaa gatgatccac
       61 atcggtaaaa acaccaaatc gaccattatc tcgaaaggga tctctgccgg acatagtcag
      121 aacagttatc gcggcttagt gaaaatcatg ccgacggcaa ccaatgcgcg caatttcact
      181 cagtgcgact caatgctgat tggcgctaat tgtggggcgc ataccttccc gtatgtcgaa
      241 tgtcgcaata acagcgcaca actggagcac gaggcaacga catcacgtat tggtgaagat
      301 caactgtttt actgcctgca acgcgggatc agcgaagaag acgccatctc gatgattgtt
      361 aacggtttct gcaaagacgt gttctcagaa ctgccgctgg aatttgccgt tgaagcacaa
      421 aaactcctcg ccatcagtct tgaacacagc gtcggataag gaataaacat gttaagtatt
      481 aaagatttac acgtcagcgt ggaagataaa gctatcctgc gtggattaag cctcgacgtt
      541 cgtcccggcg aagttcacgc
//
LOCUS       DAMZET010000423          552 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.430, whole genome shotgun sequence.
ACCESSION   DAMZET010000423 DAMZET010000000
VERSION     DAMZET010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.430"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..262
                     /locus_tag="POB34_005021"
     CDS             <1..262
                     /locus_tag="POB34_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921825.1"
                     /translation="FLAANTFPKSRETRAPLVEELYRFRDRLPEKLRYLADAPQQDPE
                     GNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYVDGKWVEGKK"
BASE COUNT          160 a          113 c          123 g          156 t
ORIGIN      
        1 gttcctagct gccaacactt tcccgaaatc gcgtgaaacg cgtgcgccac tggtggaaga
       61 gctttatcgc ttccgcgacc gtctgccgga aaaactgcgt tatctggccg atgcgccaca
      121 gcaggatccg gaaggtaata agaccatggt tcgctttagc cgtaaaacca aacagcaata
      181 tgtctcttcg gaaaaagacg gaaaggcgac tggctggtca gcattttatg ttgatggcaa
      241 atgggttgaa ggaaaaaaat aacctttaat tctgtcaggt ttttataaac aaagggtcgc
      301 gaaagcggcc cttttttatt gcatattatt ttttcttcac acctatacac taaggctata
      361 aatgatatag tggttatagt tagcaccttt tttattatta aatcgtatta gtcacccgcc
      421 aggtgtgacg aaaaaacgat gttctgatgg cgtctaagtg gatggtttaa catgaaatta
      481 caacaacttc gctatattgt tgaggtggtc aatcataacc tgaatgtctc atcaacagcg
      541 gaaggacttt ac
//
LOCUS       DAMZET010000424          539 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.431, whole genome shotgun sequence.
ACCESSION   DAMZET010000424 DAMZET010000000
VERSION     DAMZET010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.431"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>539
                     /locus_tag="POB34_005022"
     rRNA            <1..>539
                     /locus_tag="POB34_005022"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          151 a          110 c          171 g          107 t
ORIGIN      
        1 tgtgtgttag tggaagcgtc tggaaaggcg cgcgatacag ggtgacagcc ccgtacacaa
       61 aaatgcacat gctgtgagct cgatgagtag ggcgggacac gtggtatcct gtctgaatat
      121 ggggggacca tcctccaagg ctaaatactc ctgactgacc gatagtgaac cagtaccgtg
      181 agggaaaggc gaaaagaacc ccggcgaggg gagtgaaaaa gaacctgaaa ccgtgtacgt
      241 acaagcagtg ggagcacgct taggcgtgtg actgcgtacc ttttgtataa tgggtcagcg
      301 acttatattc tgtagcaagg ttaaccgaat aggggagccg aagggaaacc gagtcttaac
      361 tgggcgttaa gttgcagggt atagacccga aacccggtga tctagccatg ggcaggttga
      421 aggttgggta acactaactg gaggaccgaa ccgactaatg ttgaaaaatt agcggatgac
      481 ttgtggctgg gggtgaaagg ccaatcaaac cgggagatag ctggttctcc ccgaaagct
//
LOCUS       DAMZET010000425          533 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.432, whole genome shotgun sequence.
ACCESSION   DAMZET010000425 DAMZET010000000
VERSION     DAMZET010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.432"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>533
                     /locus_tag="POB34_005023"
     rRNA            <1..>533
                     /locus_tag="POB34_005023"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          131 a          126 c          162 g          114 t
ORIGIN      
        1 gaactttcca gagatggatt ggtgccttcg ggaactgtga gacaggtgct gcatggctgt
       61 cgtcagctcg tgttgtgaaa tgttgggtta agtcccgcaa cgagcgcaac ccttatcctt
      121 tgttgccagc ggtccggccg ggaactcaaa ggagactgcc agtgataaac tggaggaagg
      181 tggggatgac gtcaagtcat catggccctt acgaccaggg ctacacacgt gctacaatgg
      241 cgcatacaaa gagaagcgac ctcgcgagag caagcggacc tcataaagtg cgtcgtagtc
      301 cggattggag tctgcaactc gactccatga agtcggaatc gctagtaatc gtggatcaga
      361 atgccacggt gaatacgttc ccgggccttg tacacaccgc ccgtcacacc atgggagtgg
      421 gttgcaaaag aagtaggtag cttaaccttc gggagggcgc ttaccacttt gtgattcatg
      481 actggggtga agtcgtaaca aggtaaccgt aggggaacct gcggttggat cac
//
LOCUS       DAMZET010000426          526 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.433, whole genome shotgun sequence.
ACCESSION   DAMZET010000426 DAMZET010000000
VERSION     DAMZET010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.433"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..245)
                     /locus_tag="POB34_005024"
     CDS             complement(<1..245)
                     /locus_tag="POB34_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="negative modulator of the initiation of chromosome
                     replication; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation regulator SeqA"
                     /protein_id="HDI5921826.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYA"
     gene            430..>526
                     /locus_tag="POB34_005025"
     CDS             430..>526
                     /locus_tag="POB34_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDI5921827.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNL"
BASE COUNT          145 a          133 c          116 g          132 t
ORIGIN      
        1 gcgtattcgt ccgaaagcag aagttcacgc attgcgcgaa ccttgtcttt aatcgttttg
       61 accggcttcg cttcgacgat agcaggtgac gcaacgcgaa cctctttcgt caccggagca
      121 gcaggctgtg atgcggcgga aaatttcaac atacgccgta aaatgtcgga tgcgctctcg
      181 ccgatatgct tagtgtggct ggcaatatag ctgtagagtt catcatcaac ttcaatcgtt
      241 ttcatcttaa tccagtgcag tgtcttagct gaatacaagt cgccaagagt atatgaataa
      301 atcccggcag cggatagcgt caagcctggc ttgcagaaaa actcggaata aaccttattt
      361 gttgcagccc tgtggcacaa tggtcaacat aataccctaa cccgacaacg cgaaaaaaag
      421 aactttgcca tgaaattgaa tatccgcgcg caaactgcac aaaaccagca caataattct
      481 cccatcgttc ttgtccatgg tctgtttggt agcctcgaca accttg
//
LOCUS       DAMZET010000427          505 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.434, whole genome shotgun sequence.
ACCESSION   DAMZET010000427 DAMZET010000000
VERSION     DAMZET010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.434"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..133)
                     /locus_tag="POB34_005026"
     CDS             complement(<1..133)
                     /locus_tag="POB34_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Modulates the activities of several enzymes which
                     are inactive in their acetylated form; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDI5921828.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     "
     gene            complement(149..>505)
                     /locus_tag="POB34_005027"
     CDS             complement(149..>505)
                     /locus_tag="POB34_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDI5921829.1"
                     /translation="ENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQGDEQARAHVERY
                     LDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPVARVPRIERARH
                     GDAGGMRGAAFLHLTD"
BASE COUNT          136 a          139 c          118 g          112 t
ORIGIN      
        1 cgagtactct tggtttttcc attgcttccg gcaccacttt atctctgaaa aaaatacgct
       61 gacgcaaacg ctcgcgcagg cggcgtttat ttttacgaaa acgacttaac cgatgacccc
      121 gacgcgacag catagcaacc tctgttgttt aatcggttag atgtaggaag gccgcaccac
      181 gcattcctcc cgcatcaccg tggcgcgcgc gttcaatgcg cggaacacga gctacaggta
      241 agagatgacg aggcagcctg tccgccagtt gcgttgtgat tgccgggaaa ttcgataagc
      301 caccaccaat gacgaccagg tcagggtcaa caatggtcag gatatttccc agacaaaccg
      361 ctaataaatc cagataacgc tcaacgtgcg cccttgcctg ctcatcgcct tgatcataaa
      421 gcgcaataat ttcgggagcc tgcaacggtt gatgataata gtgttgatac agccacgcaa
      481 aaccgcgacc agacagataa ttttc
//
LOCUS       DAMZET010000428          501 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.435, whole genome shotgun sequence.
ACCESSION   DAMZET010000428 DAMZET010000000
VERSION     DAMZET010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.435"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>501
                     /locus_tag="POB34_005028"
     CDS             <1..>501
                     /locus_tag="POB34_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001475310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDI5921830.1"
                     /translation="DGRISGYSAGIYATWYQNDANKTGAYVDSWALYNWFDNSVSSDN
                     RSADDYDSRGVTASVEGGYTFEAGTFSGSEGTLNTWYVQPQVQITWMGVKDSDHTRKD
                     GTRIETEGDGNVQTRLGVKTYLNSHHQRDDGKQREFQPYIEANWINNSKVYAVKMNGQ
                     TVSRDG"
BASE COUNT          136 a          114 c          150 g          101 t
ORIGIN      
        1 cggatgggcg catcagcggg tacagcgctg ggatttacgc gacctggtat cagaacgatg
       61 cgaataagac cggcgcttat gttgacagct gggcgctgta taactggttt gataacagcg
      121 tcagttccga taaccgttct gctgacgact atgattctcg cggtgtgacg gcctctgttg
      181 agggtgggta tacctttgaa gcgggaacat ttagcggcag cgaagggacg ctgaatacct
      241 ggtacgtcca gccacaggtg caaatcacct ggatgggtgt gaaagattct gaccataccc
      301 ggaaagacgg aacacgcatt gaaacggaag gcgacggaaa cgtgcaaacg cgacttgggg
      361 tgaaaaccta cctgaacagc catcaccagc gtgacgatgg taaacagcgt gagttccagc
      421 cttacattga agcgaactgg atcaacaata gcaaagtcta cgccgtgaag atgaatggtc
      481 aaaccgtaag ccgtgatggt g
//
LOCUS       DAMZET010000429          489 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.436, whole genome shotgun sequence.
ACCESSION   DAMZET010000429 DAMZET010000000
VERSION     DAMZET010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.436"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
BASE COUNT          140 a          111 c          105 g          133 t
ORIGIN      
        1 aatcatcatc agcgtcgata tggacgaata taaaaacatt tacgttatat cccaaaaaca
       61 cacaggtctt agggaggttt aaactgtgta ttaacaccta tcgtattgac gggcgtgaaa
      121 tggcagaaac agaagtcgtt cctattgaca tgcctgactc taacggcgag atgacttggc
      181 aagccaaaaa ctacacacag tattcgtcct actttatgaa gattacttgc ttgaaatagc
      241 ttattggcaa atggcatata cagttattga accattctga tcttcggttg tatcgcacgc
      301 agccgaccat gagttattgg ctgatgggaa tgacctggta agccatatgt aaccagttcg
      361 tgaggtgcat attccgccgc cacctgtaac acgttttcca gttggacaag tagctgttgc
      421 tccaggaaag cgccaacctg tggcctgtgc tgtagcaatt tgaagctcac cagatcctac
      481 ctttctcca
//
LOCUS       DAMZET010000430          466 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.437, whole genome shotgun sequence.
ACCESSION   DAMZET010000430 DAMZET010000000
VERSION     DAMZET010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.437"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..76)
                     /locus_tag="POB34_005029"
     CDS             complement(<1..76)
                     /locus_tag="POB34_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HDI5921831.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHK"
     gene            complement(180..>466)
                     /locus_tag="POB34_005030"
     CDS             complement(180..>466)
                     /locus_tag="POB34_005030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921832.1"
                     /translation="SILILEIALIKQRMLANIETSNPYHAQYILRRRDDYLVLFDRDA
                     LSCRFFYDAFPGMRLRHQVTGDTDDDRLAHSPADRMYTGKDHQKYTFCTK"
BASE COUNT          127 a           89 c          110 g          140 t
ORIGIN      
        1 atttatgttc cactttccag gcttgtctgt aatcggggat aatagaaata tgttccatca
       61 attttttaag ttccattttg ttctccttaa ttatgtaagg agtatttgat catgtataag
      121 caataaaaaa cagcttcagg taatgaggaa tatctcaatt tttaaacata aaatgtgaat
      181 tatttagtac aaaaagtgta cttctgatga tctttccctg tatacatccg gtctgcgggg
      241 ctgtgtgcca ggcggtcatc gtcggtatcg ccggtcacct ggtggcgcag acgcattccc
      301 ggaaaggcgt cataaaagaa gcggcagctc agggcatcac ggtcgaacag caccaggtaa
      361 tcatcgcggc ggcgcaggat gtactgggcg tgatacgggt tactggtttc gatattggct
      421 aacatccgct gttttattaa tgcgatttct aaaatcagga tggaaa
//
LOCUS       DAMZET010000431          456 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.438, whole genome shotgun sequence.
ACCESSION   DAMZET010000431 DAMZET010000000
VERSION     DAMZET010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.438"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..>456)
                     /locus_tag="POB34_005031"
     CDS             complement(<1..>456)
                     /locus_tag="POB34_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HDI5921833.1"
                     /translation="AALWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVL
                     PAPLPPYRVLTGLVDRFGRTQTFHREAAGEFSGEITGVTDGAGRHFRLVLTTQAQRAE
                     EARQQAISGGTEPSAFPDTLPGYTEYGRDNGIRLSAVWLTHDPEYPENLP"
BASE COUNT           75 a          158 c          144 g           79 t
ORIGIN      
        1 ggtaaattct ccgggtattc cgggtcgtgc gtcagccaca cggcagacag acggatgccg
       61 ttgtcccggc catattcggt gtaacccggc agggtatcag gaaaagcgga cggttccgtc
      121 ccgccggaaa tggcctgctg ccgggcttct tctgcccgct gcgcctgcgt ggtcagtacc
      181 agccggaagt gacgcccggc accatccgtc acgccggtga tttcgccgct gaattcaccg
      241 gcggcttcgc ggtggaacgt ctgtgtgcgc ccgaagcggt ccaccagccc ggtcagtacc
      301 cggtacggcg gcagcggcgc aggcagcacc tcatccgctt ccggcacccg ctcacaccag
      361 ccgagcagcc accacggccc ctgcggactg tttgtcgcca gataacgatg cggacttaag
      421 cggagttctt ccggcagcgc ctgccagagt gcggcc
//
LOCUS       DAMZET010000432          453 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.439, whole genome shotgun sequence.
ACCESSION   DAMZET010000432 DAMZET010000000
VERSION     DAMZET010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.439"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            91..339
                     /locus_tag="POB34_005032"
     CDS             91..339
                     /locus_tag="POB34_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5921834.1"
                     /translation="MFIYASGGNGGSAGGACANTSRLQGYVGGTLISVNASNNPAYGK
                     TAFISFAVPAGTSYQITSYPTENTSCGAGVFSVFGYQT"
     gene            complement(336..>453)
                     /locus_tag="POB34_005033"
     CDS             complement(336..>453)
                     /locus_tag="POB34_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin"
                     /protein_id="HDI5921835.1"
                     /translation="GNAEDAHYCQVVESPAGSRKWYKYEHKTGCIASCVTLN"
BASE COUNT          115 a          100 c          108 g          130 t
ORIGIN      
        1 tggaagactt ctggttcgct caatggttct tacacaaact taggttcaca tagaggttca
       61 ttctcagggc ggaattcagg gggcagtaca ttgtttattt atgcatctgg aggtaatgga
      121 ggatctgccg gaggtgcatg tgcaaataca tcccgactgc agggatatgt tggtgggacg
      181 ctaattagcg tgaatgccag caataaccct gcatatggaa aaacagcctt tatcagcttt
      241 gctgtacctg caggtacttc ctatcagata acatcctatc caacagaaaa tacatcatgt
      301 ggtgccgggg tattttcagt atttggatat caaacttaat tgagcgttac acacgacgca
      361 atacaacctg tcttatgctc gtatttgtac catttgcgtg aacctgctgg gctctcaact
      421 acctggcagt agtgggcatc ctctgcgttg ccc
//
LOCUS       DAMZET010000433          433 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.440, whole genome shotgun sequence.
ACCESSION   DAMZET010000433 DAMZET010000000
VERSION     DAMZET010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.440"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..398)
                     /locus_tag="POB34_005034"
     CDS             complement(<1..398)
                     /locus_tag="POB34_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI5921836.1"
                     /translation="MSRIFAYCRISTLDQTTENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMQVISAVAEFERDLLLERT"
BASE COUNT          108 a           99 c           90 g          136 t
ORIGIN      
        1 tgagtgcgtt caagtaaaag gtctcgttca aattcagcga ctgctgaaat tacttgcatc
       61 atcatttttc ctgttggact ggtcaggtca atgcccccca atgctaagca atgcactctg
      121 atacctgttt cggtcagttg ttccactgtt ttcctgatat ccattgcatt acaaccaagg
      181 cgatccagtt ttgtcacaat caattgatca ccacatttca ggcgagcaag caaccggtta
      241 aaaccaggac gctcactggt tgctgctgag ccgctaatgt gttcttcgat tatttgctga
      301 ggtttgattt taaaacctgc actttcgatt tcccggcgtt gattttcggt ggtctgatcc
      361 agcgttgata tccgacagta agcaaaaatt cgagacatag tgagactcta tacgaaattg
      421 gtgttcatat cat
//
LOCUS       DAMZET010000434          428 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.441, whole genome shotgun sequence.
ACCESSION   DAMZET010000434 DAMZET010000000
VERSION     DAMZET010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.441"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>428
                     /gene="hipA"
                     /locus_tag="POB34_005035"
     CDS             <1..>428
                     /gene="hipA"
                     /locus_tag="POB34_005035"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HDI5921837.1"
                     /translation="KLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIK
                     AGRVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFL
                     MGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAG"
BASE COUNT          103 a           89 c          122 g          114 t
ORIGIN      
        1 ttaaattacc gattggcgaa atcaggcagc ccaatgcgac gctcgatctc agccaaagcg
       61 ttgataatga gtattactgt ctgctgctgg cgaaagaact tgggttgaat gttccggacg
      121 cagaaatcat taaagcggga agggtgcgcg cgttagcggt cgaacgtttt gacaggcgtt
      181 ggaatactga gcgaacggtt ttacttcgct tgccacagga ggatatgtgt cagacattcg
      241 gtttaccttc atctgtgaaa tatgaatcag atggaggccc aggcatcgcg cggatcatgg
      301 cgtttttgat ggggtccagc gaggcgcttc gcgatcgtta tgattttatg aaattccagg
      361 tcttccagtg gttgattggc gcaacggatg gccatgcaaa aaacttctcc gtatttattc
      421 aggctggc
//
LOCUS       DAMZET010000435          427 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.442, whole genome shotgun sequence.
ACCESSION   DAMZET010000435 DAMZET010000000
VERSION     DAMZET010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.442"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>427
                     /gene="tuf"
                     /locus_tag="POB34_005036"
     CDS             <1..>427
                     /gene="tuf"
                     /locus_tag="POB34_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDI5921838.1"
                     /translation="IDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKE
                     TQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESE
                     VYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGV"
BASE COUNT          105 a          103 c          116 g          103 t
ORIGIN      
        1 attgacaagc cgttcctgct gccgatcgaa gacgtattct ccatctccgg tcgtggtacc
       61 gttgttaccg gtcgtgtaga acgcggtatc atcaaagttg gtgaagaagt tgaaatcgtt
      121 ggtatcaaag agactcagaa gtctacctgt actggcgttg aaatgttccg caaactgctg
      181 gacgaaggcc gtgctggtga gaacgtaggt gttctgctgc gtggtatcaa acgtgaagaa
      241 atcgaacgtg gtcaggtact ggctaagccg ggcaccatca agccgcacac caagttcgaa
      301 tctgaagtgt acattctgtc caaagatgaa ggcggccgtc atactccgtt cttcaaaggc
      361 taccgtccgc agttctactt ccgtactact gacgtgactg gtaccatcga actgccggaa
      421 ggcgtag
//
LOCUS       DAMZET010000436          421 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.443, whole genome shotgun sequence.
ACCESSION   DAMZET010000436 DAMZET010000000
VERSION     DAMZET010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.443"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
BASE COUNT          112 a           84 c           71 g          154 t
ORIGIN      
        1 cgaagtttga tagctgtttc agccagaatt ttaaaccagc ttcagagcac cctgctcgat
       61 gtaacacatc gctatatttt agcggtgttg gctttccttt atagaggtaa gcaagtgtct
      121 cgtagatgcg ttttcctgtg tcattacact gaattctgtc tactttgctt tttattaaat
      181 actgtatcaa tagtcctcct tactttttaa aagggaatat caaagtcatc gtatatattt
      241 gggtaatgct tagaaaaata tgcctcaata ccatttggtc gttttagccc gtatctataa
      301 gctagtgaat ttgcaaactc aataaatgat gctcctcctg ttcttgtgtg tttctcctgg
      361 atgataagag aggcaaaatt ctctaacaca tcctcctcat tatcattagc tactttttca
      421 t
//
LOCUS       DAMZET010000437          392 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.444, whole genome shotgun sequence.
ACCESSION   DAMZET010000437 DAMZET010000000
VERSION     DAMZET010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.444"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            151..226
                     /locus_tag="POB34_005037"
     tRNA            151..226
                     /locus_tag="POB34_005037"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:185..187,aa:Glu,seq:ttc)
BASE COUNT          110 a           86 c           94 g          102 t
ORIGIN      
        1 agtgctcaca cagattgtct gatgaaaagt gaatagcaag gcgtttacgc gttgggagtg
       61 aggctgaaga gaataaggcc gttcgctttc tattaatgaa agctcaccct acacgaaaat
      121 atcacgcaac gcgtgataag caattttcgt gtccccttcg tctagaggcc caggacaccg
      181 ccctttcacg gcggtaacag gggttcgaat cccctagggg acgccacttg ctggtttgtg
      241 agtgaaagtc gccgacctta atatctcaaa actcatcttc gggtgatgtt tgagatattt
      301 gctctttaaa aatctggatc aagctgaaaa ttgaaacact gaacaacgag agttgttcgt
      361 gagtctctca aattttcgca acacgatgat ga
//
LOCUS       DAMZET010000438          364 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.445, whole genome shotgun sequence.
ACCESSION   DAMZET010000438 DAMZET010000000
VERSION     DAMZET010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.445"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>364
                     /locus_tag="POB34_005038"
     CDS             <1..>364
                     /locus_tag="POB34_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HDI5921839.1"
                     /translation="TSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLF
                     YAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIWMTDGWPLYESRLKGKLHVIS
                     KRYTQRIERHNLNLRQHLA"
BASE COUNT           82 a           92 c          113 g           77 t
ORIGIN      
        1 ttacgtcact taaaaaactc aggccgcagt cggtaacctc gcgcatacag ccgggcagtg
       61 acgtcatcgt ctgcgcggaa atggacgaac agtggggcta tgtcggggct aaatcgcgcc
      121 agcgctggct gttttacgcg tatgacagtc tccggaagac ggttgttgcg cacgtattcg
      181 gtgaacgcac tatggcgacg ctggggcgtc ttatgagcct gctgtcaccc tttgacgtgg
      241 tgatatggat gacggatggc tggccgctgt atgaatcccg cctgaaggga aagctgcacg
      301 taatcagcaa gcgatatacg cagcgaattg agcggcataa cctgaatctg aggcagcacc
      361 tggc
//
LOCUS       DAMZET010000439          359 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.446, whole genome shotgun sequence.
ACCESSION   DAMZET010000439 DAMZET010000000
VERSION     DAMZET010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.446"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(189..296)
                     /locus_tag="POB34_005039"
     CDS             complement(189..296)
                     /locus_tag="POB34_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic polypeptide LdrB"
                     /protein_id="HDI5921840.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT           77 a           93 c           92 g           97 t
ORIGIN      
        1 aagagtgcat tccggttttt caacagctgt tacagtcgtt tcatgagtgc tctggatgat
       61 gcttccagct cgggttgcca atatttactt gtggaagtga taaagacaaa aatggccgca
      121 ggctgttacc cctgcggccg gtttcgggcg catattgcca tcacggcagc ctgacgcccg
      181 ttttcacctt acttccggtt acgccaccag ccgacaatcg ctgcggtaat aattcccgcc
      241 aggatcggtg ccgccaggtc gtgccagaaa gtcatggcaa actgcgcgag cgtcatatag
      301 ccgccttgtt gtaatgacaa cattttgcgg ctattcttga agtgtctggt ttcaagatt
//
LOCUS       DAMZET010000440          334 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.447, whole genome shotgun sequence.
ACCESSION   DAMZET010000440 DAMZET010000000
VERSION     DAMZET010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.447"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>334
                     /locus_tag="POB34_005040"
     CDS             <1..>334
                     /locus_tag="POB34_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI5921841.1"
                     /translation="LYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPI
                     PEGMWGYDATAMQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLN
                     KLGINVKLE"
BASE COUNT           99 a           86 c           87 g           62 t
ORIGIN      
        1 ctgtatctga ataacagcaa agcgccactt aatcaggcgg atctgcgtcg ggccatttcc
       61 tggtctaccg attatcaggg catggttaac ggcattctga gtggtaacgg aaaacaaatg
      121 cgcggcccga ttccggaagg catgtggggc tacgatgcga cggcaatgca atacaaccat
      181 gacgaaacga aagccaaagc cgaatgggat aaagtgacga gcaaacccac cagcctgacg
      241 tttctctact ccgataacga tccgaactgg gaaccgatcg ctctggcgac acaatccagt
      301 ctcaacaagc tgggcatcaa tgtgaagctg gaaa
//
LOCUS       DAMZET010000441          334 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.448, whole genome shotgun sequence.
ACCESSION   DAMZET010000441 DAMZET010000000
VERSION     DAMZET010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.448"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>334
                     /gene="comR"
                     /locus_tag="POB34_005041"
     CDS             <1..>334
                     /gene="comR"
                     /locus_tag="POB34_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HDI5921842.1"
                     /translation="CEEKSVESALADYFAAIANCFTSKDTPAGCFMINNCTTLSPDSG
                     DIANTLKSRHAMQERTLQQFLCQRQARGEIPTHCDVTHLAEFLNCIIQGMSISAREGA
                     SLEKLMQIA"
BASE COUNT           89 a           90 c           82 g           73 t
ORIGIN      
        1 tgtgaagaga aaagcgtgga gtctgcgctg gctgactatt ttgctgccat cgccaactgc
       61 tttaccagca aagacacccc ggcaggctgc ttcatgatca acaactgcac caccctctcc
      121 ccagattcag gagatatcgc caatacgttg aaatcacgcc atgcgatgca agagcgcact
      181 ttgcagcagt ttttatgtca acgacaagcg cgcggggaaa tcccgaccca ctgtgacgtg
      241 acacatctgg cagaattcct taattgtatt attcagggga tgtcgatcag cgcacgcgaa
      301 ggtgcatcgc tggaaaaact aatgcagatt gccg
//
LOCUS       DAMZET010000442          330 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.449, whole genome shotgun sequence.
ACCESSION   DAMZET010000442 DAMZET010000000
VERSION     DAMZET010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.449"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..>330)
                     /gene="potB"
                     /locus_tag="POB34_005042"
     CDS             complement(<1..>330)
                     /gene="potB"
                     /locus_tag="POB34_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="can transport spermidine and putrescine but
                     affinity is higher for spermidine; forms a complex of
                     PotA2BCD; PotB is a membrane component; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HDI5921843.1"
                     /translation="PNLMIIGTSFLTRDDASFVKMVFTLDNYTRLLDPLYFEVLLHSL
                     NMALIATLACLVLGYPFAWFLAKLPHKVRPLLLFLLIVPFWTNSLIRIYGLKIFLSTK
                     GYLNEFLL"
BASE COUNT           95 a           75 c           99 g           61 t
ORIGIN      
        1 agcaaaaact cgttgagata gcctttggtg ctgaggaaaa ttttcagccc gtagatgcga
       61 atcagtgagt tggtccagaa gggaacaatc agcagaaaca gcagcagcgg acgcaccttg
      121 tgtggtagct tcgccagaaa ccaggcaaac gggtagccca gcaccaggca ggcgagggtg
      181 gcgatcagcg ccatattcag cgagtgcaat agcacttcaa aatagagcgg atcgagcaga
      241 cgcgtgtagt tatccagcgt aaagaccatt ttgacgaaac tggcgtcgtc gcgggtcaaa
      301 aagctggtgc caatgatcat caggttgggc
//
LOCUS       DAMZET010000443          329 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.450, whole genome shotgun sequence.
ACCESSION   DAMZET010000443 DAMZET010000000
VERSION     DAMZET010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.450"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>329
                     /locus_tag="POB34_005043"
     CDS             <1..>329
                     /locus_tag="POB34_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDI5921844.1"
                     /translation="VKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTG
                     GFNSETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKNTA
                     MRSPESNG"
BASE COUNT           77 a           75 c          113 g           64 t
ORIGIN      
        1 gtgaaagaaa gtaatcagcg atggtgctct gacgggttcg agttccgctg tgataacgga
       61 gaaaaactgc gagtcacgtt cgcgctggac tgctgtgacc gtgaggcact gcactgggcg
      121 gttactacgg gcggcttcaa cagtgaaaca gtacaggacg tcatgctggg agcggtggaa
      181 cgccgcttcg gcaacgagct tccggcgtct ccagtagagt ggctgacgga taatggttca
      241 tgctaccggg ctaatgaaac acggcagttt gcccggatgt tggggcttga accgaagaac
      301 acggcgatgc ggagtccgga gagtaacgg
//
LOCUS       DAMZET010000444          326 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.451, whole genome shotgun sequence.
ACCESSION   DAMZET010000444 DAMZET010000000
VERSION     DAMZET010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.451"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..>326)
                     /locus_tag="POB34_005044"
     CDS             complement(<1..>326)
                     /locus_tag="POB34_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HDI5921845.1"
                     /translation="DLLPLLERALAGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYR
                     LECYTPAPKRQYGYFVLPLLHRGQLVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQ
                     KGLRQA"
BASE COUNT           83 a           86 c           81 g           76 t
ORIGIN      
        1 tcgcctgacg taaccctttt tgcagcattg tcgttggttt aatgccttcc tgtaaccaca
       61 gagagataac ttcaaggatg cctgtctggc gatgcatttt ggcatccatt cgcccaacta
      121 attgcccacg atgtaataat ggcagaacaa aatagccata ctggcgtttc ggcgctgggg
      181 tatagcactc cagccggtag ctaaaatcaa aaagctgctc tgcgcgtttg cgatcccaga
      241 caacaggatc aaaaggcgaa agtaccgcgc tgtgcgttgc agtgagcttt ccggccagcg
      301 cacgctcgag tagcggcagc aaatca
//
LOCUS       DAMZET010000445          304 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.452, whole genome shotgun sequence.
ACCESSION   DAMZET010000445 DAMZET010000000
VERSION     DAMZET010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.452"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..150
                     /locus_tag="POB34_005045"
     CDS             <1..150
                     /locus_tag="POB34_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hin recombinase"
                     /protein_id="HDI5921846.1"
                     /translation="RPPVLNEEQKQVVFERIKSGVSISAIAREFKTSRQTILRAKAKL
                     QTPDI"
     gene            complement(248..>304)
                     /locus_tag="POB34_005046"
     CDS             complement(248..>304)
                     /locus_tag="POB34_005046"
                     /inference="COORDINATES: protein motif:HMM:NF014467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDI5921847.1"
                     /translation="LNRVDTSTAQDIEWPALP"
BASE COUNT           98 a           67 c           60 g           79 t
ORIGIN      
        1 cgaccacctg tgttaaatga agaacagaaa caggtggtat tcgaacgaat taagtcaggt
       61 gtaagtataa gtgccattgc ccgggaattc aaaacctcgc ggcaaaccat tttaagagcc
      121 aaagcaaaac ttcagacacc tgacatataa aaaataatct cggtgtgaga tgctttacgt
      181 cttccaagcc cccttccttg ccgtaaatgg aaagatacat ctaattatag aatttatatg
      241 ttttacccta cggcagtgct ggccattcaa tatcctgtgc agttgacgta tcaacacggt
      301 tcag
//
LOCUS       DAMZET010000446          288 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.453, whole genome shotgun sequence.
ACCESSION   DAMZET010000446 DAMZET010000000
VERSION     DAMZET010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.453"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(109..>288)
                     /locus_tag="POB34_005047"
     CDS             complement(109..>288)
                     /locus_tag="POB34_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="catalyzes the dehydration of
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic acid
                     to form O-succinylbenzoate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDI5921848.1"
                     /translation="SSSIESSLGLTQLARIAAWLTPDTIPGLDTLDLMQAQQVRRWPG
                     STLPVVEVDALERLL"
BASE COUNT           67 a           93 c           76 g           52 t
ORIGIN      
        1 cgacgcgagc acaaagctcg cgccagttaa gttgctcgtc attaagacgt aaggcgatgg
       61 cttctccccg cacttgccgc cagtgacgcc acggccagtc agagaagatc ataacaaccg
      121 ctccagtgca tcaacttcca cgacaggcag cgtgctaccc ggccagcgac gtacctgctg
      181 cgcctgcatc agatccagcg tgtccagccc tggaatggtg tccggcgtta accaggcggc
      241 aatccgcgcc agttgcgtta agcctaagct cgattcaatg gaagaact
//
LOCUS       DAMZET010000447          275 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.454, whole genome shotgun sequence.
ACCESSION   DAMZET010000447 DAMZET010000000
VERSION     DAMZET010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.454"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>275
                     /locus_tag="POB34_005048"
     CDS             <1..>275
                     /locus_tag="POB34_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDI5921849.1"
                     /translation="GNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDI
                     VWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYL"
BASE COUNT           52 a           81 c           80 g           62 t
ORIGIN      
        1 ggtaactatg agttcccaca accgctgcac gatgcgctgg ataaattcca ggccgatacc
       61 ggtatcgaca tcgacatgca catcgacgct gccagcggtg gcttcctggc accgttcgtc
      121 gccccggata tcgtctggga cttccgcctg ccgcgtgtga aatcgatcag tgcttcaggc
      181 cataaattcg gtctggctcc gctgggctgc ggctgggtta tctggcgtga cgaagaagcg
      241 ctgccgcagg aactggtgtt caacgttgac tacct
//
LOCUS       DAMZET010000448          273 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.455, whole genome shotgun sequence.
ACCESSION   DAMZET010000448 DAMZET010000000
VERSION     DAMZET010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.455"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>273
                     /locus_tag="POB34_005049"
     CDS             <1..>273
                     /locus_tag="POB34_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDI5921850.1"
                     /translation="KAGLHIGIGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDV
                     GWPGSSPMTWASQIALVAIPIAILVNVAMLLTRMTRVVNVDIWNIW"
BASE COUNT           52 a           55 c           87 g           79 t
ORIGIN      
        1 taaagcgggt ctgcatatcg ggattggctt tgttggcatt ggccttgtga ttggcttaat
       61 gctggattcc attggtccgg cggcgaaagc gatggcggaa aatttcgacc tgaatctgca
      121 tgtggtcgat gtcggctggc cgggctcttc accaatgacc tgggcttcgc aaattgcgct
      181 ggtggcgatt ccgattgcga tattggttaa cgtggcgatg ttactgaccc gtatgacgcg
      241 ggtggtaaat gttgatatct ggaatatctg gca
//
LOCUS       DAMZET010000449          259 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.456, whole genome shotgun sequence.
ACCESSION   DAMZET010000449 DAMZET010000000
VERSION     DAMZET010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.456"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
BASE COUNT           93 a           36 c           40 g           90 t
ORIGIN      
        1 gtcaataagt tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat
       61 gtgtctgcag gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag
      121 ataaagattt agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggaacttt
      181 gtgaacaccg tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt
      241 atatgattta cttatattt
//
LOCUS       DAMZET010000450          243 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.457, whole genome shotgun sequence.
ACCESSION   DAMZET010000450 DAMZET010000000
VERSION     DAMZET010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.457"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            2..>243
                     /locus_tag="POB34_005050"
     rRNA            2..>243
                     /locus_tag="POB34_005050"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           75 a           53 c           67 g           48 t
ORIGIN      
        1 aggttaagcg actaagcgta cacggtggat gccctggcag tcagaggcga tgaaggacgt
       61 gctaatctgc gataagcgtc ggtaaggtga tatgaaccgt tataaccggc gatttccgaa
      121 tggggaaacc cagtgtgatt cgtcacacta tcattaactg aatccatagg ttaatgaggc
      181 gaaccggggg aactgaaaca tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc
      241 cag
//
LOCUS       DAMZET010000451          233 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.denovo.458, whole genome shotgun sequence.
ACCESSION   DAMZET010000451 DAMZET010000000
VERSION     DAMZET010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.denovo.458"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..>233)
                     /locus_tag="POB34_005051"
     CDS             complement(<1..>233)
                     /locus_tag="POB34_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HDI5921851.1"
                     /translation="LSAFQLPQSSLLTLTQYAFLATALACFAQAFCGHRRAIMEGPGG
                     LWWGTILTITLGEASRGTPINDIATSLAVGIVLS"
BASE COUNT           62 a           65 c           69 g           37 t
ORIGIN      
        1 gagagcacaa tccccaccgc caggctggta gcgatatcgt tgatcggtgt cccgcgcgat
       61 gcttcgccaa gggtgatagt aaggatggtt ccccaccaca ggccacccgg cccttccata
      121 atagcgcgac gatgaccgca gaacgcctga gcgaagcagg ccagtgcggt agcaagaaaa
      181 gcatattgcg tgagcgtaag caggctactt tgcggcaact gaaaagcaga aag
//
LOCUS       DAMZET010000452         1971 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.guided.346, whole genome shotgun sequence.
ACCESSION   DAMZET010000452 DAMZET010000000
VERSION     DAMZET010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.guided.346"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..598)
                     /gene="tetR(A)"
                     /locus_tag="POB34_005052"
     CDS             complement(<1..598)
                     /gene="tetR(A)"
                     /locus_tag="POB34_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HDI5921852.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLA"
     gene            704..1903
                     /gene="tet(A)"
                     /locus_tag="POB34_005053"
     CDS             704..1903
                     /gene="tet(A)"
                     /locus_tag="POB34_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HDI5921853.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
BASE COUNT          314 a          631 c          613 g          413 t
ORIGIN      
        1 tcgccaatcc atcgacaatc accgcgagtc cctgctcgaa cgctgcgtcc ggaccggctt
       61 cgtcgaaggc gtctatcgcg gcccgcaaca gcggcgagag cggagcctgt tcaacggtgc
      121 cgccgcgctc gccggcatcg ctgtcgccgg cctgctcctc aagcacggcc ccaacagtga
      181 agtagctgat tgtcatcagc gcattgacgg cgtccccggc cgaaaaaccc gcctcgcaga
      241 ggaagcgaag ctgcgcgtcg gccgtttcca tctgcggtgc gcccggtcgc gtgccggcat
      301 ggatgcgcgc gccatcgcgg taggcgagca gcgcctgcct gaagctgcgg gcattcccga
      361 tcagaaatga gcgccagtcg tcgtcggctc tcggcaccga atgcgtatga ttctccgcca
      421 gcatggcttc ggccagtgcg tcgagcagcg cccgcttgtt cctgaagtgc cagtaaagcg
      481 ccggctgctg aacccccaac cgttccgcca gtttgcgtgt cgtcagaccg tctacgccga
      541 cctcgttcaa caggtccagg gcggcacgga tcactgtatt cggctgcaac tttgtcatgc
      601 ttgacacttt atcactgata aacataatat gtccaccaac ttatcagtga taaagaatcc
      661 gcgcgttcaa tcggaccagc ggaggctggt ccggaggcca gacgtgaaac ccaacagacc
      721 cctgatcgta attctgagca ctgtcgcgct cgacgctgtc ggcatcggcc tgattatgcc
      781 ggtgctgccg ggcctcctgc gcgatctggt tcactcgaac gacgtcaccg cccactatgg
      841 cattctgctg gcgctgtatg cgttgatgca atttgcctgc gcacctgtgc tgggcgcgct
      901 gtcggatcgt ttcgggcggc ggccggtctt gctcgtctcg ctggccggcg ctgctgtcga
      961 ctacgccatc atggcgacgg cgcctttcct ttgggttctc tatatcgggc ggatcgtggc
     1021 cggcatcacc ggggcgactg gggcggtagc cggcgcttat attgccgata tcactgatgg
     1081 cgatgagcgc gcgcggcact tcggcttcat gagcgcctgt ttcgggttcg ggatggtcgc
     1141 gggacctgtg ctcggtgggc tgatgggcgg tttctccccc cacgctccgt tcttcgccgc
     1201 ggcagccttg aacggcctca atttcctgac gggctgtttc cttttgccgg agtcgcacaa
     1261 aggcgaacgc cggccgttac gccgggaggc tctcaacccg ctcgcttcgt tccggtgggc
     1321 ccggggcatg accgtcgtcg ccgccctgat ggcggtcttc ttcatcatgc aacttgtcgg
     1381 acaggtgccg gccgcgcttt gggtcatttt cggcgaggat cgctttcact gggacgcgac
     1441 cacgatcggc atttcgcttg ccgcatttgg cattctgcat tcactcgccc aggcaatgat
     1501 caccggccct gtagccgccc ggctcggcga aaggcgggca ctcatgctcg gaatgattgc
     1561 cgacggcaca ggctacatcc tgcttgcctt cgcgacacgg ggatggatgg cgttcccgat
     1621 catggtcctg cttgcttcgg gtggcatcgg aatgccggcg ctgcaagcaa tgttgtccag
     1681 gcaggtggat gaggaacgtc aggggcagct gcaaggctca ctggcggcgc tcaccagcct
     1741 gacctcgatc gtcggacccc tcctcttcac ggcgatctat gcggcttcta taacaacgtg
     1801 gaacgggtgg gcatggattg caggcgctgc cctctacttg ctctgcctgc cggcgctgcg
     1861 tcgcgggctt tggagcggcg cagggcaacg agccgatcgc tgatcgtgga aacgataggc
     1921 ctatgccatg cgggtcaagg cgacttccgg caagctatac gcgccctagg a
//
LOCUS       DAMZET010000453         1566 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.guided.365, whole genome shotgun sequence.
ACCESSION   DAMZET010000453 DAMZET010000000
VERSION     DAMZET010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.guided.365"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            89..1222
                     /locus_tag="POB34_005054"
     CDS             89..1222
                     /locus_tag="POB34_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDI5921854.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            1311..>1566
                     /gene="blc"
                     /locus_tag="POB34_005055"
     CDS             1311..>1566
                     /gene="blc"
                     /locus_tag="POB34_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDI5921855.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREM"
BASE COUNT          411 a          401 c          380 g          374 t
ORIGIN      
        1 cgctgattgg tatcgttaca atctaacgta tcgccaatgt aaatccggcc cgcctatggc
       61 gggccgtttt gtatggaaac cagaccttat gttcaaaacg acgctctgcg ccttattaat
      121 taccgcctct tgctccacat ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac
      181 aattaccccg cttatagagc aacaaaagat ccccggtatg gcggtggcgg taatttatca
      241 gggtaaacct tattacttta cctggggcta tgcggacatc gccaaaaagc agcccgtcac
      301 acagcaaacg ttgtttgagt taggttcggt cagcaaaaca tttacgggcg tgcttggtgg
      361 cgacgctatt gctcgagggg aaatcaagtt aagcgatccc gcaacaaaat actggcctga
      421 acttaccgct aaacagtgga atgggatcac actattacat cttgcgacct acaccgctgg
      481 cggcctgcca ttgcaggtgc cggatgaagt gaaatcctca agcgacttgc tgcgcttcta
      541 tcaaaactgg cagcctgcat gggctccagg aacacaacgt ctgtatgcca actccagtat
      601 cggtttgttc ggcgcactgg ctgtgaagcc gtctggtttg agttttgagc aggcgatgca
      661 aactcgtgtc ttccagccac tcaaactcaa ccatacgtgg attaatgtac ctcccccaga
      721 agaaaagaat tacgcctggg gatatcgcga aggtaaggca gtgcatgttt cgccaggggc
      781 gttagatgct gaagcttatg gtgtgaagtc gaccattgaa gatatggccc gctgggtacg
      841 aagcaatatg aatccccgtg atatcaacga caaaacactt cagcaaggga tacaactggc
      901 acaatctcgc tactggcaaa ccggcgatat gtatcagggc ctgggctggg aaatgctgga
      961 ctggccggta aatcctgaca gcatcattaa cggcagtggc aataaaattg cactggcagc
     1021 acaccctgta aaagcgatta cgcccccaac tcctgcagta cgcgcatcat gggtacataa
     1081 aacaggggca accggcggat ttggtagcta tgtcgcgttt attccagaaa aagagctggg
     1141 tatagtgatg ctggctaaca aaaactatcc caatccagcg agagtcgccg ccgcctggca
     1201 gattctcaac gctctacagt aaaatttcat cgggtccgaa ttttcggacc ttttctgcgc
     1261 ttttccttgc tgtcatctac acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc
     1321 tccctctcgt tgccgcagcg acagctgcat ttctggtcgt tgcctgcagt tctcctacgc
     1381 cgccgcgtgg cgtaacagta gtgaataatt tcgacgcaaa acgttatctc ggtacctggt
     1441 atgagattgc ccgttttgat caccgttttg aacgtggact ggaaaaagtc accgcaacat
     1501 acagtctgcg tgatgacggc ggcctgaatg tcatcaataa aggctataac cctgacagag
     1561 aaatgt
//
LOCUS       DAMZET010000454         1505 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.guided.367, whole genome shotgun sequence.
ACCESSION   DAMZET010000454 DAMZET010000000
VERSION     DAMZET010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.guided.367"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..181
                     /locus_tag="POB34_005056"
     CDS             <1..181
                     /locus_tag="POB34_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
                     /protein_id="HDI5921856.1"
                     /translation="GVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLL
                     ARSPSWKSLRRPTPW"
     gene            175..1014
                     /gene="sul1"
                     /locus_tag="POB34_005057"
     CDS             175..1014
                     /gene="sul1"
                     /locus_tag="POB34_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HDI5921857.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            1142..>1505
                     /locus_tag="POB34_005058"
     CDS             1142..>1505
                     /locus_tag="POB34_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5921858.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAEDPEFWPDALKGEAAYLHKLAVRRTHA
                     GRGVSSALIEACRHAARTQ"
BASE COUNT          259 a          461 c          484 g          301 t
ORIGIN      
        1 cggtgttgct tatgcagtct ggtcgggact cggcgtcgtc ataattacag ccattgcctg
       61 gttgcttcat gggcaaaagc ttgatgcgtg gggctttgta ggtatggggc tcataattgc
      121 tgcctttttg ctcgcccgat ccccatcgtg gaagtcgctg cggaggccga cgccatggtg
      181 acggtgttcg gcattctgaa tctcaccgag gactccttct tcgatgagag ccggcggcta
      241 gaccccgccg gcgctgtcac cgcggcgatc gaaatgctgc gagtcggatc agacgtcgtg
      301 gatgtcggac cggccgccag ccatccggac gcgaggcctg tatcgccggc cgatgagatc
      361 agacgtattg cgccgctctt agacgccctg tccgatcaga tgcaccgtgt ttcaatcgac
      421 agcttccaac cggaaaccca gcgctatgcg ctcaagcgcg gcgtgggcta cctgaacgat
      481 atccaaggat ttcctgaccc tgcgctctat cccgatattg ctgaggcgga ctgcaggctg
      541 gtggttatgc actcagcgca gcgggatggc atcgccaccc gcaccggtca ccttcgaccc
      601 gaagacgcgc tcgacgagat tgtgcggttc ttcgaggcgc gggtttccgc cttgcgacgg
      661 agcggggtcg ctgccgaccg gctcatcctc gatccgggga tgggattttt cttgagcccc
      721 gcaccggaaa catcgctgca cgtgctgtcg aaccttcaaa agctgaagtc ggcgttgggg
      781 cttccgctat tggtctcggt gtcgcggaaa tccttcttgg gcgccaccgt tggccttcct
      841 gtaaaggatc tgggtccagc gagccttgcg gcggaacttc acgcgatcgg caatggcgct
      901 gactacgtcc gcacccacgc gcctggagat ctgcgaagcg caatcacctt ctcggaaacc
      961 ctcgcgaaat ttcgcagtcg cgacgccaga gaccgagggt tagatcatgc ctagcattca
     1021 ccttccggcc gcccgctagc ggaccctggt caggttccgc gaaggtgggc gcagacatgc
     1081 tgggctcgtc aggatcaaac tgcactatga ggcggcggtt cataccgcgc caggggagcg
     1141 aatggacagc gaggagcctc cgaacgttcg ggtcgcctgc tcgggtgata tcgacgaggt
     1201 tgtgcggctg atgcacgacg ctgcggcgtg gatgtccgcc aagggaacgc ccgcctggga
     1261 cgtcgcgcgg atcgaccgga cattcgcgga gaccttcgtc ctgagatccg agctcctagt
     1321 cgcgagttgc agcgacggca tcgtcggctg ttgcaccttg tcggccgagg atcccgagtt
     1381 ctggcccgac gccctcaagg gggaggccgc atatctgcac aagctcgcgg tgcgacggac
     1441 acatgcgggc cggggtgtca gctccgcgct gatcgaggct tgccgccatg ccgcgcgaac
     1501 gcagg
//
LOCUS       DAMZET010000455         1130 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.guided.392, whole genome shotgun sequence.
ACCESSION   DAMZET010000455 DAMZET010000000
VERSION     DAMZET010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.guided.392"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..136)
                     /locus_tag="POB34_005059"
     CDS             complement(<1..136)
                     /locus_tag="POB34_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDI5921859.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     I"
     gene            285..1076
                     /gene="aadA1"
                     /locus_tag="POB34_005060"
     CDS             285..1076
                     /gene="aadA1"
                     /locus_tag="POB34_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HDI5921860.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          274 a          278 c          322 g          256 t
ORIGIN      
        1 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
       61 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
      121 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
      181 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
      241 atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgagg gaagcggtga
      301 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
      361 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
      421 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
      481 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
      541 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
      601 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
      661 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
      721 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
      781 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
      841 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
      901 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
      961 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     1021 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc
     1081 taacaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc
//
LOCUS       DAMZET010000456          987 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.guided.401, whole genome shotgun sequence.
ACCESSION   DAMZET010000456 DAMZET010000000
VERSION     DAMZET010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.guided.401"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            40..939
                     /gene="aac(3)-VIa"
                     /locus_tag="POB34_005061"
     CDS             40..939
                     /gene="aac(3)-VIa"
                     /locus_tag="POB34_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(3)-VIa"
                     /protein_id="HDI5921861.1"
                     /translation="MTDPRKNGDLHEPATAPATPWSKSELVRQLRDLGVRSGDMVMPH
                     VSLRAVGPLADGPQTLVDALIEAVGPTGNILAFVSWRDSPYEQTLGHDAPPAAIAQSW
                     PAFDPDHAPTYPGFGAINEFIRTYPGCRRSAHPDASMAAIGPDAAWLVAPHEMGAAYG
                     PRSPIARFLAHAGKILSIGAGPDAVTALHYAEAVARIEGKRRVTYSMPLLREGKRVWV
                     TTSDWDSNGILDEYAAPDGPDAVERIARDYLARTRVAQGPVGGAQSRLIDAADIVSFG
                     IEWLEARHAAPAAAALKPKQRRD"
BASE COUNT          172 a          346 c          302 g          167 t
ORIGIN      
        1 tgcataataa gccctacaca aattgggaga tatatcatga tgactgatcc ccgcaaaaac
       61 ggcgatttgc acgaacccgc gacggcaccc gcgacgccct ggtccaaaag cgagctggtc
      121 cggcaattgc gcgacctcgg cgtgcgctca ggcgatatgg tgatgccgca tgtgtcgttg
      181 cgcgccgtcg ggccgctggc ggacggaccg cagacacttg tcgatgcgct gatcgaggcc
      241 gtcggcccca ccgggaatat tctcgccttc gtctcgtggc gcgattcgcc ctatgaacag
      301 acgctgggtc atgatgcgcc gcccgccgcc atcgcccaaa gctggcctgc gttcgacccc
      361 gaccatgcgc ccacctaccc cggctttggc gcgatcaacg aatttatccg aacctatccg
      421 gggtgtcggc gcagcgccca tcccgacgca tcgatggcgg cgatcgggcc cgatgcggcg
      481 tggctggtgg cgccgcacga gatgggcgcc gcttatggcc cccgctcgcc gatcgcgcgt
      541 tttctcgccc acgcaggaaa aatcctgtcg atcggcgccg ggcccgatgc agtcaccgcg
      601 ctccattatg ccgaagcggt ggcgcggatc gagggcaagc gccgcgtcac ttattcgatg
      661 cccttactgc gcgaaggcaa gcgcgtctgg gtcaccacgt ccgactggga ttcgaacggc
      721 atcctcgacg aatatgccgc gcccgacggc cccgacgcgg tcgaacggat cgcccgcgac
      781 tatctcgccc gcaccagggt tgcgcaaggc ccggtcggcg gcgcgcaatc ccggctgatc
      841 gacgcggccg atatcgtttc cttcggcatc gaatggctcg aggcgcgcca cgccgcgcca
      901 gcggcggcag cgctgaagcc gaaacaacgc cgcgactgag tcgtcgatta ttccggacat
      961 atattcctcc ctctcgccca gccgcat
//
LOCUS       DAMZET010000457          833 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.guided.412, whole genome shotgun sequence.
ACCESSION   DAMZET010000457 DAMZET010000000
VERSION     DAMZET010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.guided.412"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            37..384
                     /locus_tag="POB34_005062"
     CDS             37..384
                     /locus_tag="POB34_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HDI5921862.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            378..>833
                     /locus_tag="POB34_005063"
     CDS             378..>833
                     /locus_tag="POB34_005063"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDI5921863.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRF"
BASE COUNT          161 a          235 c          231 g          206 t
ORIGIN      
        1 tgcataataa gccctacaca aattgggaga tatatcatga aaggctggct ttttcttgtt
       61 atcgcaatag ttggcgaagt aatcgcaaca tccgcattaa aatctagcga gggctttact
      121 aagcttgccc cttccgccgt tgtcataatc ggttatggca tcgcatttta ttttctttct
      181 ctggttctga aatccatccc tgtcggtgtt gcttatgcag tctggtcggg actcggcgtc
      241 gtcataatta cagccattgc ctggttgctt catgggcaaa agcttgatgc gtggggcttt
      301 gtaggtatgg ggctcataat tgctgccttt ttgctcgccc gatccccatc gtggaagtcg
      361 ctgcggaggc cgacgccatg gtgacggtgt tcggcattct gaatctcacc gaggactcct
      421 tcttcgatga gagccggcgg ctagaccccg ccggcgctgt caccgcggcg atcgaaatgc
      481 tgcgagtcgg atcagacgtc gtggatgtcg gaccggccgc cagccatccg gacgcgaggc
      541 ctgtatcgcc ggccgatgag atcagacgta ttgcgccgct cttagacgcc ctgtccgatc
      601 agatgcaccg tgtttcaatc gacagcttcc aaccggaaac ccagcgctat gcgctcaagc
      661 gcggcgtggg ctacctgaac gatatccaag gatttcctga ccctgcgctc tatcccgata
      721 ttgctgaggc ggactgcagg ctggtggtta tgcactcagc gcagcgggat ggcatcgcca
      781 cccgcaccgg tcaccttcga cccgaagacg cgctcgacga gattgtgcgg ttc
//
LOCUS       DAMZET010000458          578 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2369
            SAMN32919495-rid16881083.guided.427, whole genome shotgun sequence.
ACCESSION   DAMZET010000458 DAMZET010000000
VERSION     DAMZET010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919495
            Sequence Read Archive: SRR23232116
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591610.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591610.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:32:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 53 of 148
            Pseudo Genes (incomplete)         :: 74 of 148
            Pseudo Genes (internal stop)      :: 41 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919495-rid16881083.guided.427"
                     /strain="UMNRS2369"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            137..>578
                     /gene="gpt"
                     /locus_tag="POB34_005064"
     CDS             137..>578
                     /gene="gpt"
                     /locus_tag="POB34_005064"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDI5921864.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDNYVVDIPQDTWIEQPWDMGVVFVP"
BASE COUNT          142 a          137 c          153 g          146 t
ORIGIN      
        1 gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga
       61 tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca
      121 ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca
      181 tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt
      241 aagccgtggc ggtctggtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt
      301 cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa
      361 acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg
      421 tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tctttgcaaa
      481 accggctggt cgtccgctgg ttgataatta tgttgttgat atcccgcaag atacctggat
      541 cgaacagccg tgggatatgg gcgtcgtatt cgtcccgc
//
